LOCUS       DAZFRG010000001        65772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFRG010000001 DAZFRG010000000
VERSION     DAZFRG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.001"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..454)
                     /gene="folK"
                     /locus_tag="ACHKUC_000001"
     CDS             complement(<1..454)
                     /gene="folK"
                     /locus_tag="ACHKUC_000001"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO6616335.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAK"
     gene            complement(451..1848)
                     /gene="pcnB"
                     /locus_tag="ACHKUC_000002"
     CDS             complement(451..1848)
                     /gene="pcnB"
                     /locus_tag="ACHKUC_000002"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO6616336.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1908..2804)
                     /gene="gluQRS"
                     /locus_tag="ACHKUC_000003"
     CDS             complement(1908..2804)
                     /gene="gluQRS"
                     /locus_tag="ACHKUC_000003"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO6616337.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2871..3326)
                     /gene="dksA"
                     /locus_tag="ACHKUC_000004"
     CDS             complement(2871..3326)
                     /gene="dksA"
                     /locus_tag="ACHKUC_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO6616338.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3504..4208)
                     /gene="sfsA"
                     /locus_tag="ACHKUC_000005"
     CDS             complement(3504..4208)
                     /gene="sfsA"
                     /locus_tag="ACHKUC_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO6616339.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4223..4753)
                     /gene="thpR"
                     /locus_tag="ACHKUC_000006"
     CDS             complement(4223..4753)
                     /gene="thpR"
                     /locus_tag="ACHKUC_000006"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO6616340.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIRWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4827..7256
                     /gene="hrpB"
                     /locus_tag="ACHKUC_000007"
     CDS             4827..7256
                     /gene="hrpB"
                     /locus_tag="ACHKUC_000007"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO6616341.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCGDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLARAYADRIARRRGQDE
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7350..9878
                     /gene="mrcB"
                     /locus_tag="ACHKUC_000008"
     CDS             7350..9878
                     /gene="mrcB"
                     /locus_tag="ACHKUC_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO6616342.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVMIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQLLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10098..12356
                     /gene="fhuA"
                     /locus_tag="ACHKUC_000009"
     CDS             10098..12356
                     /gene="fhuA"
                     /locus_tag="ACHKUC_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO6616343.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDQEALKASPCLSIPQSQWGHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSE
                     RDDKEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12407..13204
                     /gene="fhuC"
                     /locus_tag="ACHKUC_000010"
     CDS             12407..13204
                     /gene="fhuC"
                     /locus_tag="ACHKUC_000010"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO6616344.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13204..14094
                     /gene="fhuD"
                     /locus_tag="ACHKUC_000011"
     CDS             13204..14094
                     /gene="fhuD"
                     /locus_tag="ACHKUC_000011"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO6616345.1"
                     /translation="MSGLPLISRRRLLTAMALSPFLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLTHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPYVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14091..16073
                     /gene="fhuB"
                     /locus_tag="ACHKUC_000012"
     CDS             14091..16073
                     /gene="fhuB"
                     /locus_tag="ACHKUC_000012"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO6616346.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGTRRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16108..17388)
                     /gene="hemL"
                     /locus_tag="ACHKUC_000013"
     CDS             complement(16108..17388)
                     /gene="hemL"
                     /locus_tag="ACHKUC_000013"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO6616347.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17554..17601
                     /gene="yadX"
                     /locus_tag="ACHKUC_000014"
     CDS             17554..17601
                     /gene="yadX"
                     /locus_tag="ACHKUC_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO6616348.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17613..19034
                     /gene="clcA"
                     /locus_tag="ACHKUC_000015"
     CDS             17613..19034
                     /gene="clcA"
                     /locus_tag="ACHKUC_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO6616349.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHCVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19058..19123
                     /gene="yadW"
                     /locus_tag="ACHKUC_000016"
     CDS             19058..19123
                     /gene="yadW"
                     /locus_tag="ACHKUC_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO6616350.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19116..19460
                     /gene="erpA"
                     /locus_tag="ACHKUC_000017"
     CDS             19116..19460
                     /gene="erpA"
                     /locus_tag="ACHKUC_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO6616351.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19507..20130)
                     /gene="yadS"
                     /locus_tag="ACHKUC_000018"
     CDS             complement(19507..20130)
                     /gene="yadS"
                     /locus_tag="ACHKUC_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO6616352.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20168..20968)
                     /gene="btuF"
                     /locus_tag="ACHKUC_000019"
     CDS             complement(20168..20968)
                     /gene="btuF"
                     /locus_tag="ACHKUC_000019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO6616353.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIGQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20961..21659)
                     /gene="mtnN"
                     /locus_tag="ACHKUC_000020"
     CDS             complement(20961..21659)
                     /gene="mtnN"
                     /locus_tag="ACHKUC_000020"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO6616354.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21743..23260
                     /gene="dgt"
                     /locus_tag="ACHKUC_000021"
     CDS             21743..23260
                     /gene="dgt"
                     /locus_tag="ACHKUC_000021"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO6616355.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVTAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23390..24814
                     /gene="degP"
                     /locus_tag="ACHKUC_000022"
     CDS             23390..24814
                     /gene="degP"
                     /locus_tag="ACHKUC_000022"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO6616356.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTSQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24969..26126
                     /gene="cdaR"
                     /locus_tag="ACHKUC_000023"
     CDS             24969..26126
                     /gene="cdaR"
                     /locus_tag="ACHKUC_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO6616357.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26180..26566)
                     /gene="yaeH"
                     /locus_tag="ACHKUC_000024"
     CDS             complement(26180..26566)
                     /gene="yaeH"
                     /locus_tag="ACHKUC_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO6616358.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26728..27540)
                     /gene="yaeI"
                     /locus_tag="ACHKUC_000025"
     CDS             complement(26728..27540)
                     /gene="yaeI"
                     /locus_tag="ACHKUC_000025"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO6616359.1"
                     /translation="MISRRRFLQATAATIATGSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASKANLPRLVLAHNPDSKEVMRDDPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27594..28418)
                     /gene="dapD"
                     /locus_tag="ACHKUC_000026"
     CDS             complement(27594..28418)
                     /gene="dapD"
                     /locus_tag="ACHKUC_000026"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO6616360.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28449..31121)
                     /gene="glnD"
                     /locus_tag="ACHKUC_000027"
     CDS             complement(28449..31121)
                     /gene="glnD"
                     /locus_tag="ACHKUC_000027"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO6616361.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31183..31977)
                     /gene="map"
                     /locus_tag="ACHKUC_000028"
     CDS             complement(31183..31977)
                     /gene="map"
                     /locus_tag="ACHKUC_000028"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO6616362.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32345..33070
                     /gene="rpsB"
                     /locus_tag="ACHKUC_000029"
     CDS             32345..33070
                     /gene="rpsB"
                     /locus_tag="ACHKUC_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO6616363.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33205..34056
                     /gene="tsf"
                     /locus_tag="ACHKUC_000030"
     CDS             33205..34056
                     /gene="tsf"
                     /locus_tag="ACHKUC_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO6616364.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34203..34928
                     /gene="pyrH"
                     /locus_tag="ACHKUC_000031"
     CDS             34203..34928
                     /gene="pyrH"
                     /locus_tag="ACHKUC_000031"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO6616365.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35078..35635
                     /gene="frr"
                     /locus_tag="ACHKUC_000032"
     CDS             35078..35635
                     /gene="frr"
                     /locus_tag="ACHKUC_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO6616366.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35727..36923
                     /gene="ispC"
                     /locus_tag="ACHKUC_000033"
     CDS             35727..36923
                     /gene="ispC"
                     /locus_tag="ACHKUC_000033"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO6616367.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVARMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37112..37870
                     /gene="ispU"
                     /locus_tag="ACHKUC_000034"
                     /gene_synonym="uppS"
     CDS             37112..37870
                     /gene="ispU"
                     /locus_tag="ACHKUC_000034"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO6616368.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37883..38740
                     /gene="cdsA"
                     /locus_tag="ACHKUC_000035"
     CDS             37883..38740
                     /gene="cdsA"
                     /locus_tag="ACHKUC_000035"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6616369.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38752..40104
                     /gene="rseP"
                     /locus_tag="ACHKUC_000036"
     CDS             38752..40104
                     /gene="rseP"
                     /locus_tag="ACHKUC_000036"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO6616370.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40134..42566
                     /gene="bamA"
                     /locus_tag="ACHKUC_000037"
     CDS             40134..42566
                     /gene="bamA"
                     /locus_tag="ACHKUC_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO6616371.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42688..43173
                     /gene="skp"
                     /locus_tag="ACHKUC_000038"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42688..43173
                     /gene="skp"
                     /locus_tag="ACHKUC_000038"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO6616372.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43177..44202
                     /gene="lpxD"
                     /locus_tag="ACHKUC_000039"
     CDS             43177..44202
                     /gene="lpxD"
                     /locus_tag="ACHKUC_000039"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6616373.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDCVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44307..44762
                     /gene="fabZ"
                     /locus_tag="ACHKUC_000040"
     CDS             44307..44762
                     /gene="fabZ"
                     /locus_tag="ACHKUC_000040"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO6616374.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44766..45554
                     /gene="lpxA"
                     /locus_tag="ACHKUC_000041"
     CDS             44766..45554
                     /gene="lpxA"
                     /locus_tag="ACHKUC_000041"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO6616375.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45554..46702
                     /gene="lpxB"
                     /locus_tag="ACHKUC_000042"
     CDS             45554..46702
                     /gene="lpxB"
                     /locus_tag="ACHKUC_000042"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO6616376.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46699..47295
                     /gene="rnhB"
                     /locus_tag="ACHKUC_000043"
     CDS             46699..47295
                     /gene="rnhB"
                     /locus_tag="ACHKUC_000043"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO6616377.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47332..50814
                     /gene="dnaE"
                     /locus_tag="ACHKUC_000044"
     CDS             47332..50814
                     /gene="dnaE"
                     /locus_tag="ACHKUC_000044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO6616378.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50827..51786
                     /gene="accA"
                     /locus_tag="ACHKUC_000045"
     CDS             50827..51786
                     /gene="accA"
                     /locus_tag="ACHKUC_000045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO6616379.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51884..54025
                     /gene="ldcC"
                     /locus_tag="ACHKUC_000046"
     CDS             51884..54025
                     /gene="ldcC"
                     /locus_tag="ACHKUC_000046"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO6616380.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54082..54471
                     /gene="yaeR"
                     /locus_tag="ACHKUC_000047"
     CDS             54082..54471
                     /gene="yaeR"
                     /locus_tag="ACHKUC_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6616381.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54536..55849
                     /gene="tilS"
                     /locus_tag="ACHKUC_000048"
     CDS             54536..55849
                     /gene="tilS"
                     /locus_tag="ACHKUC_000048"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO6616382.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTQLIRPLLARTR
                     GELVQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETILPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55883..56143)
                     /gene="rof"
                     /locus_tag="ACHKUC_000049"
     CDS             complement(55883..56143)
                     /gene="rof"
                     /locus_tag="ACHKUC_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO6616383.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLALELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56130..56330)
                     /gene="yaeP"
                     /locus_tag="ACHKUC_000050"
     CDS             complement(56130..56330)
                     /gene="yaeP"
                     /locus_tag="ACHKUC_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO6616384.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56496..57041
                     /gene="yaeQ"
                     /locus_tag="ACHKUC_000051"
     CDS             56496..57041
                     /gene="yaeQ"
                     /locus_tag="ACHKUC_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO6616385.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57038..57460
                     /gene="arfB"
                     /locus_tag="ACHKUC_000052"
     CDS             57038..57460
                     /gene="arfB"
                     /locus_tag="ACHKUC_000052"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO6616386.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57474..58184
                     /gene="nlpE"
                     /locus_tag="ACHKUC_000053"
                     /gene_synonym="cutF"
     CDS             57474..58184
                     /gene="nlpE"
                     /locus_tag="ACHKUC_000053"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO6616387.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(58340..59164)
                     /gene="yaeF"
                     /locus_tag="ACHKUC_000054"
     CDS             complement(58340..59164)
                     /gene="yaeF"
                     /locus_tag="ACHKUC_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO6616388.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59217..60935)
                     /gene="proS"
                     /locus_tag="ACHKUC_000055"
     CDS             complement(59217..60935)
                     /gene="proS"
                     /locus_tag="ACHKUC_000055"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO6616389.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGNASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(61046..61753)
                     /gene="tsaA"
                     /locus_tag="ACHKUC_000056"
     CDS             complement(61046..61753)
                     /gene="tsaA"
                     /locus_tag="ACHKUC_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO6616390.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61750..62154)
                     /gene="rcsF"
                     /locus_tag="ACHKUC_000057"
     CDS             complement(61750..62154)
                     /gene="rcsF"
                     /locus_tag="ACHKUC_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO6616391.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62272..63087)
                     /gene="metQ"
                     /locus_tag="ACHKUC_000058"
     CDS             complement(62272..63087)
                     /gene="metQ"
                     /locus_tag="ACHKUC_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO6616392.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63127..63780)
                     /gene="metI"
                     /locus_tag="ACHKUC_000059"
     CDS             complement(63127..63780)
                     /gene="metI"
                     /locus_tag="ACHKUC_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO6616393.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63773..64804)
                     /gene="metN"
                     /locus_tag="ACHKUC_000060"
     CDS             complement(63773..64804)
                     /gene="metN"
                     /locus_tag="ACHKUC_000060"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO6616394.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64992..65564
                     /gene="gmhB"
                     /locus_tag="ACHKUC_000061"
     CDS             64992..65564
                     /gene="gmhB"
                     /locus_tag="ACHKUC_000061"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO6616395.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRNVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15500 a        16353 c        18117 g        15802 t
ORIGIN      
        1 cttttgcctg tagagcttca actaatgata agccatcagg aaataccaat tccggcgcaa
       61 tttcaaacag tggccacagc ataaagccac gatttttcat atcgtagtgc ggtacggtca
      121 gacgttcggt atttattact tcattaccaa acagcatgat gtcgagatcc agcgtgcgcg
      181 gtccccagcg ttcagcttta cggacgcgac cttgctgcaa ttcgatacgt tgtgtgtgat
      241 tgagtagctc ttcaggggca agagtggttt ccagcgcgac ggcggcattt aagtaatcgg
      301 gttgatcttg cggccccagc ggtggggtgc ggtaaaacga agaaacagca agaatgcggc
      361 tttctggaat atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca
      421 gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg
      481 tttgcgtgga cgacgagtac gacgacgcgg tgacggttct tcatccagtt cgttaagcat
      541 ccctttttgg tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgttg
      601 cagttcagcg ttacgctcaa cttcggctcg taaggccaac aggtcgtatg ccgcacggaa
      661 cttaggatgc tccagcagtt tccatgcgcg ttttccctga cgacgggaca tacgcaactg
      721 caactgccag atatcgcggg ttagcgtggt cagccgtttc gggatcgcca gtgaacggca
      781 ggcttcgtcc agcacgtcgt tcatcgccag tgcgaaagcg tcgtgatagg tcaggccgct
      841 ttcctgggcg atcttctgtg ccgtctccag caatgggtac cagaacatgg cggcaaacag
      901 gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagtacctg
      961 ctcaataatg cgttccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg
     1021 gaacagcggc tggaacagat gatattcaca cagcagctta taggtttcgt aaccgtagcc
     1081 cgcttgcaac agtttcagcg attcttcaaa cagacgtgcc ggtgggatat cgttcagtaa
     1141 ggttgccagg cgtgggatcg gctcggcggt ttccgggctg atgcgcatgc ccaatttggc
     1201 ggcaaaacga accgcgcgaa gcatccgtac cggatcttca cggtagcgcg tttccgggtt
     1261 accaatcaga cggataacgc cgtctttcag atccttcatg ccgccaacgt aatcacggac
     1321 ggtaaaatct gccacgctgt aatacaggct gttgatagtg aaatcgcggc gctgggcgtc
     1381 ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacata ccgttttgcc cgcgttggga
     1441 ggtcgtgcgg tcgctgacgt taccttcgtg atgtccacgg aaggtcgcaa cttcgataat
     1501 ctccgggcca aacatcacat gagccagacg gaaacggcga cccaccaggc ggcagttacg
     1561 gaacagtttg cgcacctgct ctggcgtggc gttggtggtc acgtcaaaat ctttcggctt
     1621 tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt
     1681 attgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg
     1741 ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc
     1801 ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg
     1861 tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca
     1921 tttgagaatg ttgaatttac aattgccgac tcaggcacgg cggtcagcgt ccagtttttg
     1981 acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgtttc tacctgctga
     2041 cccagaaaat gaagtgccgc gattaacacc gggcgcggat cgcctttcgg caacgcaggc
     2101 gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg taaatggatg
     2161 taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccgcac cgtcggttcg
     2221 atcaaatctg ccccgcggac aatttcgctt acgccctgga agtgatcatc caccaccacc
     2281 gccaggttat aggcaaacaa accatcacgg cgatgaataa taaaatcttc ccgtgccagt
     2341 ttttcgtcgg cgtgaataat gcctcgcagc aggtcagtaa actgggtgac cggatgctgc
     2401 tggcggatac gtactgcggc gttgtctggt ccatgatgca aatcccggca atgaccgtcg
     2461 taaataccgc caatgctttg aatacgcgca cgcgtgcagg tgcagtaata acttagtcct
     2521 tgctcatgta accaggcgag tgcttcgcga taggcgtggt gacgttgcga ttgccagaga
     2581 acgtcgccgt cccagtacag gccgtaatgt tccagctggc gcaggatagt ttctgcggca
     2641 ccgggaactt cacgaggcgg gtcgatatct tcaatgcgta ccagccagcg accttgccgg
     2701 gcgcgagcct gcaaatagct gccaagcgca gcaatcagag aaccaaaatg aagctcgccg
     2761 gaaggagagg gggcgaagcg gccaatatag tgtgtgtctg tcatctcttt gaacaaaaaa
     2821 taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc
     2881 atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg
     2941 cgcgcttcca gacggcgaat accgatttca acaccgcagg attcgcagta gccgaaatct
     3001 tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagtttacg ctcgcgatcg
     3061 cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg
     3121 aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga
     3181 ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct
     3241 tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat
     3301 gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca
     3361 tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc
     3421 taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt
     3481 aaattaacca gttacttact ctactacaat gtaaccggta gtgatttttt aagagccatg
     3541 ccttcggcag aaagttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct
     3601 gacaatagtt gcgcgtattt ctcatcaatg tggcgcgcgg gtgaaaaccg tgtaatggct
     3661 gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac
     3721 tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc
     3781 tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac
     3841 ataaagtcaa tacggctgcg ttcggcgccg tattttactt cgcttttcag cgagctatag
     3901 cctgccagct ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa
     3961 agcgtattga cgcaaataat tgcgccgctc tggctttgag ttaattccca ggtgtgtggg
     4021 tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa
     4081 ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc
     4141 acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagaa
     4201 aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc
     4261 gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gtatacgacc
     4321 agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat
     4381 gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gagcggagca
     4441 tattcgccag ctggattaag ccgcgaggcg gctgtcgcat ccctaaccac accacacgcg
     4501 aacgcagcca ttgaccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc
     4561 cggctaaaag agaaagcgcc ttctctttct ctgcgctcac ttcgcctaaa aatgccagag
     4621 tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc
     4681 gccagcggat aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagccgtt
     4741 gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga
     4801 agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttacctg
     4861 agttacttgc tgccctcgat ggtgcatcgc aggtgttatt aagtgcgccg accggggccg
     4921 ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta
     4981 tcctgctgga gccgcgtcgt ctggcggcgc gtaacgttgc gcaacgactg gcggagctgc
     5041 ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc
     5101 cgaatacccg cctggaagtg gttaccgaag gcgtgctgac gcggatgatc cagcgtgacc
     5161 cggaactgag tggtgttgga ttggtgatcc ttgatgaatt tcatgagcgc agcttgcagg
     5221 cggatttggc attggcgttg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac
     5281 tgctgattat gtcggctacg ctggacaacg accgcttgca gcaaatgctg ccagaagcgc
     5341 ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccgttatttg ccgctgcccg
     5401 ctcatcagcg ttttgacgaa gccgtcgcgg ttgctaccgc cgaaatgctg cgtcaggaaa
     5461 gtggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac
     5521 tgacttcacg catcggcagt gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga
     5581 acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga
     5641 ccaatattgc tgaaacaagt ttaaccatcg aaggtattcg tctggtggtg gattgtgccc
     5701 aggaaagggt ggcgcgtttt gatccgcgta cagggcttac gcggttgatt actcaacgcg
     5761 ttagtcaggc gtcgatgacg caacgtgccg ggcgtgccgg gcgtctggag ccgggtatct
     5821 gcctgcattt aatcgccaaa gaacaagcag aacgcgctgc cgcgcaaagt gaaccggaga
     5881 tcttacaaag cgatctttcc ggtttgctga tggaattact gcaatgggga tgcggcgatc
     5941 cggcgcagat gagctggctg gatcaaccgc caacggtgaa tctactggcc gcgaaacgcc
     6001 tgttacagat gctgggggcg ctggatggcg aacgcctaag cgcgcaaggg caaaaaatgg
     6061 cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg
     6121 aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata
     6181 gtgacctggg cgtggcgttt tcgcgcaatc aacccgcctg gcagcaacgt agtcagcaac
     6241 tgttaaaacg cttaaacgta cgcggcggtg aggcagacag ttcgcttatc gcgccgctac
     6301 ttgccagagc gtatgccgat cgcattgctc gtcgccgtgg gcaagatgaa cgctatcaac
     6361 tggcgaacgg catgggggcg atgctcgatg ccgacgatgc gctaagccgc cacgaatggt
     6421 tgatcgcacc gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgc
     6481 tgccggtcga tattgatgag ttagtacaac gctgcccgca gctggtacag cagtctgaca
     6541 ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaattggtc
     6601 agttgatggt aaaagtgcaa ccgctggcga aaccctcgga agacgagttg catcaggcga
     6661 tgcttaacgg cattcgtgat aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac
     6721 agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg
     6781 ttgatgatga aagtttgttg gcgacgctgg aaacgtggct actgccgcat atgacaggcg
     6841 tacattcgct acgcggcctg aaatcactcg atatttatca ggcactgcgt ggattacttg
     6901 attgggtaat gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg
     6961 gaagccggat cgccattcgt taccatgaag ataacccgcc cgcgctggcg gtgagaatgc
     7021 aggagatgtt tggtgaggcc actaatccga ctatcgccca ggggcgcgta ccgttggtgc
     7081 tggagttgct ttcccctgcc caaaggccgc tgcaaatcac gcgtgatttg agcgccttct
     7141 ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct
     7201 ggccggacga cccggcaaac accgcaccga cgcgacgaac gaaaaagtat tcgtgaagac
     7261 caaagcaaat ttgagagata tcttcttctg tcttgcaaca gaagaacaga aaatcgggct
     7321 tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag ccaattggac
     7381 gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg
     7441 acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg cgcaaaggta
     7501 agggcaaagg gcgtaagcct cgtggcaaac gcggctggct atggctgctg ctaaaactgg
     7561 ctatcgtttt tgccgtgatg atcgccattt acggcgttta tctcgatcaa aaaattcgca
     7621 gccgtattga tggcaaggtc tggcaattgc ccgcggcagt ttatggtcga atggtcaacc
     7681 ttgagccaga catgaccatc agcaagaacg agatggtgaa gctgctggag gcgacccagt
     7741 atcgccaggt ctcgaaaatg acccgtcctg gcgagtttac cgtacaggcc aacagcattg
     7801 agatgatccg ccgtccgttt gatttcccgg acagcaaaga agggcaggtg cgcgcgcgtc
     7861 tgacctttga tggcgatcat ctggcgacga tcgtcaatat ggagaacaac cgtcagttcg
     7921 gtttcttccg tcttgatccg cgcctgatca ccatgatttc ttcgccaaac ggtgagcagc
     7981 gtctgtttgt gccgcgcagt ggtttcccgg atttgctggt ggatactttg ttggcgacag
     8041 aagaccgtca tttctacgag catgatggga tcagtctcta ctcaatcgga cgtgcggtgc
     8101 tggcaaacct gactgccgga cgcacggtgc agggggcgag tacgctgacg caacagttgg
     8161 ttaaaaacct gttcctctcc agcgagcgtt cttactggcg taaagcgaac gaagcctaca
     8221 tggcgctgat catggacgcg cgttacagca aagaccgtat tcttgagctg tatatgaacg
     8281 aggtgtatct cggtcagagc ggtgacaacg aaatccgcgg cttcccgctg gcgagcttgt
     8341 attactttgg tcgcccggta gaagagctaa gcctcgatca gcaggcgctg ttagtcggta
     8401 tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc gaaactggcg ctggagcgac
     8461 gtaatctggt actacgtttg ctgcaacagc aacaaattat tgatcaagaa ctctacgaca
     8521 tgctgagtgc gcgtccgctg ggtgttcagc cgcgcggtgg ggtgatctcc cctcagccag
     8581 cctttatgca gctggtgcgt caggagctgc aggcgaaatt aggcgataag gtaaaagatc
     8641 tctccggcgt gaagatcttc actacctttg actcggtggc ccaggacgcg gcagaaaaag
     8701 ccgctgtgga aggcattccg gcactgaaga aacagcgtaa gttgagcgat cttgaaaccg
     8761 cgattgtggt tgttgaccgc tttagcggtg aagttcgtgc gatggtcggt ggctctgagc
     8821 cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt tcccttgcaa
     8881 aaccagcgac ttatctaacg gccttaagcc agccgaaaat ctatcgtttg aatacgtgga
     8941 ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca ggtctggtca ccgcagaacg
     9001 atgatcgtcg ttatagcgaa agtggcaggg tgatgctggt agatgcgttg acccgttcga
     9061 tgaacgtgcc gacggtgaat ctgggcatgg cgttggggct gcctgcggtt acggagacct
     9121 ggattaaact gggtgtgccg aaagatcaac tgaatccagt tccagcaatg ttgttggggg
     9181 cgttgaactt aacgccaatc gaagtggcgc aggccttcca gactatcgcc agcggtggta
     9241 atcgtgctcc gctttccgcg ctacgttcag tcatcgcaga agacggcaag gtgttgtatc
     9301 agagcttccc gcaggcggaa cgcgctgttc cggcgcaggc ggcgtatctg acactatgga
     9361 ccatgcagca ggttgtacaa cgcggtacgg gacgtcagct tggggcgaaa tacccgaacc
     9421 tgcatctggc agggaaaaca gggactacca acaataacgt agatacctgg tttgcgggca
     9481 ttgatgggag cacggtgacc atcacctggg tcggccgtga taacaaccag ccgaccaaac
     9541 tgtatggtgc cagcggggca atgtcgattt atcagcgtta tctggctaac cagacgccaa
     9601 cgccgctgaa tcttgttccg ccagaagata ttgcagatat gggcgtggac tacgacggca
     9661 actttgtttg cagcggtggc atgcgtgtct tgccggtctg gaccagcgat ccgcaattgc
     9721 tgtgccagca gagtgagatg cagcagcaac cgtcaggcaa tccgtttgat cagtcttctc
     9781 agccgcagca acagccgcaa cagcaacctg ctcagcaaga gcagaaagac agcgacggtg
     9841 tagccggttg gattaaagat atgtttggga gtaattaacg tctaagcgtg agataccgga
     9901 tggcgagctg ccatccggta aaataacatc ccctccaaag tattaaccct ttcttttcat
     9961 ctgattgttt attgaccctt caggaatgcg cagattgcgt accgcttgcg aacccgccag
    10021 cgtttcgaat attatctcat ctttataata atcattctcg tttacgttat cattcacttt
    10081 acaccagaga aataccaatg gcaccttcca aaactgctca gccaaaacac tcactgcgta
    10141 aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca caggcagcgg
    10201 ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa gaaagcgcat
    10261 gggggccggc tgcaactatt gcggcgcgac agtcagctac cggcactaaa accgatacgc
    10321 cgattcaaaa agtacctcaa tctatttctg tcgtcaccgc tgaagagatg gcgctgcatc
    10381 agcctaagtc ggtgaaagaa gcgcttagct atacccctgg cgttgctgtg ggaacccgtg
    10441 gcgcatctaa tacttatgat tacctgatca tccgcggatt tgcggcagac ggccaaagcc
    10501 agaataacta tctgaatggc ctgaagttgc agggcaactt ctataacgat gcggtcatcg
    10561 acccgtatat gctggaacgt gctgaaatta tgcgtggccc ggtttccgtg ctttacggta
    10621 aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg tccgaccact gaaccgttga
    10681 aagaagttca gtttaaggcc gggactgaca gcctgttcca gaccggtttt gacttcagcg
    10741 atgcactgga tgatgacggc gtttattctt atcgcctgac cggtcttgcg cgttctgcca
    10801 atgcccagca gaaagggtca gaagagcagc gttatgctat tgcaccggcg ttcacctggc
    10861 gtccggacga taaaaccaat ttcaccttcc tttcttactt ccagaacgag ccggaaactg
    10921 gttattacgg ctggctgccg aaagagggga ccgttgagcc gctgccaaac ggtaagcgtc
    10981 tgccgacaga ctttaacgaa ggggcgaaga acaacaccta ttctcgtaac gagaagatga
    11041 ttggttatag cttcgaccac gaatttaacg acacctttac tgtgcgtcag aacctgcgct
    11101 ttgcgcaaaa taaagtttcg caaaagagcg tatatggcta cggtatgtgt tcagatccgc
    11161 tgtataccaa agaccaggag gcactcaagg cgagtccatg tttaagtatc ccgcagtctc
    11221 aatggggcca tacactgact cgtcagtacg ttatcgataa tgagaagtta gaaaacttct
    11281 ctgttgatac tcagttgcaa agcaaattcg caaccggttc tgtggatcac actctgctga
    11341 ctggcgttga ctttatgcgt atgcgtaatg acatcgactc ctggttcgga tatgccggtt
    11401 cagttgcacc atctgatatc tataatttag accgtagtga ctttgatttt ggcgctcacc
    11461 cgaatccgtc tggtccatac cgcgttctgc tgaaacagaa acaaaccggc ctgtatgttc
    11521 aggatcaggc gcagtgggat aaagtgctgg ttacgcttgg cggtcgctat gactgggccg
    11581 atcagtcatc tttcaaccgt gattacggta ataaatctga acgtgacgac aaagagttca
    11641 cctggcgtgg tggtgtaaac tacctgttcg acaacggcgt aacgccttac ttcagctaca
    11701 gtgaatcatt tgaacccgct tcacagactg atgcgaacgg tgatctgttt gcaccttcga
    11761 aaggcaaaca gtatgaagtc ggcgtgaaat atgtgccaga agatcgccca attgtggtga
    11821 ctggcgcact gtatcagctt accaaaacta ataacctgat ggcggatccg aacggttcac
    11881 tgttctcggt tgaaggcggt gagattcgtg cgcgtggcgt agagctggaa gcgaaagcgg
    11941 cactgtcggc gagtgttaac gtagtcggtt cttatactta caccgatgcg gaatacacta
    12001 ccgatactac ctacaaaggc aatacgcctg cacaggtgcc aaaacacatg gcttcgctgt
    12061 gggctgacta taccttcttt gacggtccgc tttcaggtct gacgctgggc accggtggtc
    12121 gttatactgg ctccagctat ggtgatccgg ctaactcctt taaagtggga agttatacgg
    12181 tcgtggatgc gttagtgcgt tatgatctgg cgcgagtcgg catggcgggc tccaacgtgg
    12241 cgctgcatgt caacaacctg tttgatcgtg aatacgtcgc cagctgcttt aacacttatg
    12301 gttgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc
    12361 tcttttgggg cacggatttc cgtgcccgtt tcacaagttg gctgttatgc aggaatacac
    12421 gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac
    12481 gcttttgcat ccgttgtcgt taacctttcc tgccgggaaa gtgactggtc tgattggtca
    12541 caacggttct ggtaaatcca ctctgctcaa aatgcttggt cgtcaccagc caccgtcgga
    12601 aggggagatt cttcttgatg cccaaccgct ggaaagctgg agcagcaaag cgtttgcccg
    12661 caaagtggct tatttaccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact
    12721 ggtggcgatt ggtcgttacc cgtggcatgg cgcactaggg cgctttggtg cggcagatcg
    12781 cgaaaaggtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt
    12841 cgatagcctt tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga
    12901 tagccgctgt ctgttgctcg acgaaccgac ttcggcgctg gatatcgccc accaggttga
    12961 tgtactggcg ctggtgcatc gtttaagtca ggagcgtggc ctgacggtca ttgccgtgct
    13021 gcacgatatc aatatggcgg cgcgctactg tgattatctg gtcgccctgc gcggcggtga
    13081 aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta
    13141 tggcatcccg atgggtattc tgccgcatcc ggcgggtgct gcacctgtga gttttgttta
    13201 ttgatgagcg gcttacctct tatttcgcgc cgccgactgt taacggcgat ggcgctttct
    13261 ccgttcttat ggcagatgaa taccgcccac gcggcagtta ttgatcccaa tcgtattgtg
    13321 gcactggagt ggttgccggt ggagttactg ctggcgctcg gcatcgtgcc ttacggcgtg
    13381 gcggacacca tcaactatcg cctgtgggtc agcgaaccgc cattgccgga ctcagtgatt
    13441 gacgttggtt tgcgtacaga acccaacctt gaactgctga ccgaaatgaa accgtcgttt
    13501 atggtctggt ctgcaggata tggcccttca ccagaaatgc tggcacgtat tgcgccgggg
    13561 cgcggattta acttcagcga cggcaaacag ccactggcga tggcgcgtaa atcactgacg
    13621 gaaatggcag atttacttaa cctgcaaagc gcagcggaaa cgcatttaac gcactatgaa
    13681 gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttattgctg
    13741 acgacgctta tcgatccgcg ccatatgttg gtcttcggtc caaacagctt gttccaggaa
    13801 attctcgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt
    13861 accgccgtca gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat
    13921 cacgacaaca gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg
    13981 tatgttcgcg ccggacgctt tcagcgcgtt ccggcggtct ggttctatgg tgcgacgctt
    14041 tctgcaatgc actttgtgcg tattctggat aacgccatcg gaggtaaagc gtgagtaaac
    14101 gaattgcgct ttttccggtg ttattgctgg cgctgctggt ggttgctgct gcggcgttga
    14161 cctggatgaa cttctcgcag gcgctgccgc gcagccagtg ggcgcaggcc gcctggtcgc
    14221 cggatattga cgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga
    14281 tttcgctgct ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag caagtgctgc
    14341 gtaacccgct ggcggaaccg acgacattgg gtgttgcaac aggcgcgcaa ctggggatta
    14401 ccgtcaccac gctctgggca atccccggcg cgatggcgag ccagtttgct gcgctggcag
    14461 gggcttgtgt tgttggctta atcgtctttg gcgtcgcgtg ggggaaacgt ctttcgccgg
    14521 taacgctgat cctcgcgggg ctggtagtga gcctttattg cggcgcaatc aatcagttac
    14581 tggttatctt ccatcatgac caactgcaaa gcatgttcct gtggagcacc ggaacgctga
    14641 cgcaaaccga ctggggcggc gttgaacgtt tatggccgca gctgctgggc ggcgtgatgc
    14701 tgacgttatt gctacttcgc ccgttaactc tgatggggct tgatgatggc gtggcgcgca
    14761 atctcgggct ggccttgtcg ctcgctcgtc tggcggcgtt gtcgctggcg attgtcatca
    14821 gtgcgctgct ggtgaacgcg gtggggatta tcggctttat cgggttgttc gcgccactgc
    14881 tggcgaaaat gctggggacg cggcgtttgt tgccacggtt gatgctggca tcgctgattg
    14941 gtgcgttaat tctgtggctt tctgatcaaa tcatcctctg gctgactcgc gtgtggatgg
    15001 aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggttgctgc
    15061 cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcgactgaac
    15121 gccaacacgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg
    15181 tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg
    15241 aggatttgat gccctggcgc tggccgcgaa ttatggctgc gctgtttgcg ggcgtcatgc
    15301 tggcggtggc gggctgtatt attcagcggc tgaccggaaa cccgatggca agcccggaag
    15361 tgctggggat tagctccggc gcggcgtttg gcgtggtgtt aatgctgttt ctggtgccgg
    15421 gtaatgcctt tggctggctg ttacccgcag gcagtctcgg ggcggcggtg acgctgttga
    15481 tcattatgat cgccgccgga cgtggtggat tttccccaca ccgcatgtta ctggcgggaa
    15541 tggcgttaag taccgcgttc accatgcttt tgatgatgtt gcaggcaagt ggtgacccgc
    15601 gaatggcgca agttctgacc tggatttccg gttcgaccta caacgcgacc gatgcgcagg
    15661 tctggcgtac cggaattgtg atggtgattt tgctggcgat taccccgctg tgccgccgct
    15721 ggctgaccat tttaccgctg ggtggtgata ccgcacgagc cgtggggatg gcgctgacgc
    15781 cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg acaatgacca
    15841 ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggctttc
    15901 gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtgttcg
    15961 ctgactggtg tgggcggatg gtattgtttc cattccagat cccggcgggg ctgctgtcga
    16021 cctttatcgg cgcgccatat tttatctatt tgttgagaaa gcagagccgt taatagaatg
    16081 cctcgcccgg tgggcgaggc aaaatcatta caacttcgca aacacccgac gtgcagcatc
    16141 gatggtgtta ttgatatctt ccatgctatg cgccacggac ataaagcccg cttcaaacgc
    16201 tgacggtgcc aggtaaacac cttcgtccag catcatatgg aagaaacgct taaagcgttc
    16261 cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg taaagaaaat
    16321 accgaacatg ccgccaacgt ggtttaccac cagcggaatt ccggcttctt ctgccgcttc
    16381 ccgcagacct tctgccagac gtgttgtcag ctcatccaga gtctcgtgaa cgcccggctg
    16441 cgcgacttca ttcagacagg cgaaaccggc tgccatcgca atcgggttac cggaaagcgt
    16501 acccgcctga tagaccggac cggtcggggc cagcgcatcc attacatcac gacgaccacc
    16561 gaatgcgcct accggcattc caccgccgat gattttgccg aggcaggtga gatccggttc
    16621 cacaccgtaa taatcctgtg cgccagctag cgctacgcgg aaaccggtca tcacttcatc
    16681 gatgatcagc aacgcgccaa attcgtcaca cagcgcacgc agacctggca ggaactctgg
    16741 cagcggtgga acgcagttca tattgcctgc caccggctcg acgataatac aggcaatctc
    16801 ttgcgggtat tgctcaaacg cggcgcgtac agaggccaga tcgttataag tacaggttaa
    16861 ggtatgtttg gcgaaatctg ccggaacgcc cggcgaattt ggctggccta acgtgagtgc
    16921 gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt
    16981 aataattttg tcgcgaccgg taaaaccacg ggccaggcgg atggcgctca tcgtcgcttc
    17041 agtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg tcaccagttg
    17101 cgccattttc acttccattt cggttggtgc accaaagctt aaaccacgct cggcggcttc
    17161 aatcacggca ttgcggatcg ccggatggtt atggcccagc accatcggcc cccaggaacc
    17221 gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt ccgctttttc
    17281 gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt tcacaccgcc
    17341 agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg agggttcctg
    17401 attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt aaatactcgt
    17461 caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa aagttttgtg
    17521 catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca
    17581 tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc tctttagaaa
    17641 caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt gagcgcgata
    17701 aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc gggctggctg
    17761 cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg gcactggtac
    17821 atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg gcggtgctgg
    17881 cgatgttcgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt tcggggatcc
    17941 cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt gtgttgccgg
    18001 tgaaattctt tggcgggctg ggaacactcg gcggaggcat ggtgttgggg cgcgaagggc
    18061 caaccgtgca gataggcggc aatatcgggc ggatggtact cgatgttttc cgcctgaaag
    18121 gtgacgaagc tcgtcatacg ctgttggcaa ccggtgctgc tgcggggctg gctgcggcct
    18181 ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg cagtttcgct
    18241 atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc attatgtacc
    18301 ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgat gcgccgctta
    18361 atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc cctattttta
    18421 ataaatgggt gctggggatg caggatttgc tgcactgtgt gcacggtggc aatattacca
    18481 aatgggtact gatgggtggt gcgattggcg gtctgtgtgg attgctgggg tttgtggcac
    18541 cagcaacttc gggcggcggt tttaacctga ttcctatcgc taccgcgggg aatttcagca
    18601 tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac caccttactc tgcttctctt
    18661 ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg ctgggaaccg
    18721 ctttcggaat ggttgccgtt gagttgtttc cgcaatatca ccttgaggcg gggacgtttg
    18781 ctattgccgg aatgggggcg ttactggcgg catctattcg cgcgccgtta acggggatca
    18841 ttctggttct ggaaatgacc gataactacc agctcatttt gccaatgatt attaccggtc
    18901 ttggcgcaac actattagca caatttaccg gtgggaaacc gctatactcg gcgattcttg
    18961 cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc gcatcagcca
    19021 gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta ttgggttaga
    19081 atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta gcactgccgc
    19141 tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac gaagataacc
    19201 cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc cagtatggtt
    19261 tcacctttga tgatcaggtg aatgaaggcg atatgaccat cgaaaaacag ggcgttggcc
    19321 tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac tataccgaag
    19381 gtctggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagtacc tgcggttgcg
    19441 gttcttcctt tagtatctaa tctgctgttc atgtgccgga taaactatcc ggcacatctc
    19501 ctctcttcaa cgaccattct catccagcgc aaacgtcggt agcttaagat gccaacgaat
    19561 agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg tttccagtgg
    19621 tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac aggcagtggc
    19681 gtagatttct gtacgtaaaa tcatggggat ttcgcgggcc agtacatcac gaatgatccc
    19741 gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg cttccgcatt
    19801 aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt ccagcaccgg
    19861 caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca gcatgctggt
    19921 gaccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc cgtgatccag
    19981 cgccatgtcg cgaattgttc cgccgcctac tgcggtaacc acgcccaata ccagaacgcc
    20041 aaaagggtcc atacgcaatt ttccggctaa caaaacgcca gagatagcaa ataccgctgt
    20101 gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca cctgaaaagg
    20161 tcaggcacta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg aggataatac
    20221 gtgggcttgc acgttcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20281 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct
    20341 gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccagggaacc cggctgtctt
    20401 taaagatgtt ttctccgcca caaacttcga gtacctggtt ctgaatcgac tcttttccac
    20461 tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20521 attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt tcggccttat
    20581 ccggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20641 tcgcatcgac ccacatcact tttattccca gcgaagccag ctggtcaacc tgccgctcgg
    20701 cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg cgttccagat
    20761 tcatcccctg ccaggtggaa acctgcccaa tcttttgcgc ttgtggagga tagtcggaat
    20821 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    20881 aaagcgtgat gacgcgcggc gcggcattga gccacagcgg cgcgagaaaa gacagggcga
    20941 ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    21001 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21061 tcggccacgt cggagatggc gcgtactacg acaaacggga cgttgaaatt gtggcagaca
    21121 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21181 ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca
    21241 gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21301 ttgaagcctg ccgggcagcc tggtaactga ccgtattcat aaccaaatgc cgtgacatcc
    21361 gcgtcgtgat aacgcgcttc gtccgagaca acgatatcgc ccactttcag cgttggtgcc
    21421 aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc caacagcaaa
    21481 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21541 ccattcagtt ggccggtata gatttcgcaa ccgcccagac tgatagtttg acggttttcg
    21601 attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21661 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21721 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21781 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagattctgc ggatcttcga
    21841 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca
    21901 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    21961 ccagcaggtg gggcgctaca tcgctaaaga aattttaagc cgtttgaaag agcttaaatt
    22021 actggaggcg tatggtctcg atgaactgac cgggcccttt gaaagcattg ttgagatgtc
    22081 atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cggcgataaa
    22141 tgactggttt cgccagcgtt tgtacccgga agatgccgaa agccagcctc tgactgacga
    22201 tcgctgcagc gtgacggcac tacgtttacg ggacggggaa gaaccgctta acgcgctgcg
    22261 gcgcaagatt cgtcaggact tatgtcattt tgaaggtaac gcacagggta ttcgtttggt
    22321 gcatacattg atgcggatga atctcacctg ggcacaggtt ggcggtattt taaaatatac
    22381 ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22441 gggctattat ctttccgaag aagcctatat tgcccggttg cgtaaagaac ttaatttggc
    22501 gctttacagt cgttttccgt taacgtggat tatggaagct gccgacgaca tctcctattg
    22561 tgtggcagac cttgaagatg cggtagagaa aaggatattt accgttgagc agctttatca
    22621 tcatttgcac gaggcgtggg gccagcatga gaaaggttcg ctcttttcgc tggtggttga
    22681 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22741 tatgtattta cgggtaaaca ccctaaataa actggtaccc tatgcggcac aacgatttat
    22801 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    22861 cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca
    22921 tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    22981 ttatcgtcct ttattaaacc tgccgttatc agactttact gaactggtag aaaaagaacg
    23041 ggtgaaacgt ttccctattg aaacgcgctt attccacaaa ctctcgacgc gccatcggct
    23101 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23161 atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23221 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga
    23281 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat
    23341 ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac
    23401 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23461 aacggcggct gagacttctt cagcaacgac atcccagcag atgccaagcc ttgcaccgat
    23521 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23581 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23641 ggagggttct ccgttccaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg
    23701 cggccagcaa cagaaattca tggcgctggg ttccggcgtt atcattgatg ccgataaagg
    23761 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccaactgag
    23821 cgatggtcgt aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct
    23881 gatccagatc cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcgct
    23941 gcgcgtgggt gattacaccg tagcaattgg taacccgttc ggcctgggcg agacagtaac
    24001 ttccgggatt gtctctgcgc tgggacgtag cggcctgaac gctgaaaact acgaaaactt
    24061 catccagact gatgcagcga tcaaccgggg taactcaggt ggtgcgttgg ttaacctgaa
    24121 cggcgaactg attggtatca acaccgcgat tctcgcaccg gacggcggaa acatcggtat
    24181 cggttttgct atcccgagca acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24241 ccaggtgaaa cgtggtgagt tgggtattat gggcactgag ctgaactccg atctggcgaa
    24301 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc cgaattcttc
    24361 cgccgcgaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccaat
    24421 cagcagcttt gccgcactgc gtgctcaggt gggcactatg ccggtaggta gcaaactgac
    24481 cctgggctta ctgcgcgacg ggaagcaggt taacgtgaac ctggaacttc agcagagcag
    24541 ccagaatcag gttgattcca gcaccatctt caacggcatt gaaggcgctg agatgagtaa
    24601 caaaggcaaa gatcagggcg tggtggtgaa caacgtgaaa acgggcactc cggctgcgca
    24661 gatcggcctg aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat
    24721 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24781 cggcgacagc accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaaaacg
    24841 ggaaggggtt ctccttacaa tctgtgaact ttaccacaac tccatacatc ttcatcatcc
    24901 tttgggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataatg
    24961 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta
    25021 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca
    25081 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggagc attgctggta ctttcacaag
    25141 gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg cggcagggga
    25201 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25261 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25321 agtcgcggtt gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga
    25381 acctcattca ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga
    25441 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg
    25501 tggacagcgc aatggcggag ttacagcaac tgcaaaatgc gctgactacg cccgagcgta
    25561 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25621 cttttgggcg ctgggacgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    25681 tgaaagagta tggccagctg cgttttcgcg tttcgctggg caactatttt accggtcctg
    25741 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    25801 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    25861 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    25921 acaataacgg cttgctgcgg cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc
    25981 tggcaacgtc aaaggcgttg tttattcatc gtaatactct ggaatatcgg cttaatcgta
    26041 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    26101 cgttacaact ggatgaagag cggtaggtcg attacaaaat tacagccggg tgagacttta
    26161 cccggcccac agaatggcat tatttacgcg tcagtttttc cagatcggct tcaatctcgc
    26221 tgatcttatt ggttactact gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa
    26281 gatcaacttc gctgcggtca cgctggcaga tttgatcaag ctcatcaatg atataccgta
    26341 gattcgggct aatttcctgg acttctttat aaccctgacc cacaccatca gcgacgaccg
    26401 ttttacgctg acgcgggtat ttaaacttca cgctcttggc gaaaaactcg cctttgtctt
    26461 tctggaaata gattttcaga atatcgttgt tggcttcctg ccggaggctg taacgatcaa
    26521 tttcttcagg attggtaata cccagacttt tcagattgtc gtacatagcg ttacctcaaa
    26581 atgagtcagt aaattgtgct tattttagca tttggccagg cccgcccggc tcgactgtga
    26641 tcgcagaaag cctttttctt ccagacttct atcatggcgt aaaaatcaaa aaattacctg
    26701 ctttattgtg gtgataaaat tcacgttcta caccagttcc agcatcgtta cttccgggcg
    26761 gcagttcaga cgcaaaccat acaaactgcc cacgccacgg gttgtgtaga tgtgtcgttc
    26821 gccaaaggca tttaatccgg cgacgtaacg tttatcttcg acaggggcaa aaggttcacc
    26881 aaccaacggt acgcgcagtt gcccgccgtg agtatggccg cacagcatca gatcccaggg
    26941 atcatcacgc atgacttctt tgctgtcagg attatgcgcc agcaccagtc ttggcagatt
    27001 ggctttgctg gcgggaggcg gtttgcattg tccggcccat aagtcaccgg tgcctaccag
    27061 ttcgaattgc ctgttcggcg tggcgatcac cgtagcctgg ttaaacaaca ccgtgatgcc
    27121 cactgatttc aacgtctcgc caattaagtg atttttttct gtaccaacag ggcgatcgtg
    27181 gttgccgaag caggcaaacg tcggcgcaca ttcggcaagg ggggagagta cgtcactaaa
    27241 cgccgaaaaa ttcagcggca tatcaaataa tacgtaatcg ccgcccagta atatcaaatc
    27301 gggcttttgt tctatgccaa gagcaatcgc gtcagaaatc aggcttaaag gaacaaaacg
    27361 agagtaatgg agatcggcca gaaaaagaat tttgaatggt gctgcgttgt ctttaaaaaa
    27421 ggcgaggcgg tgacggatta attcaaacca gcctggctcc cagtaatgca tataaccgaa
    27481 acctgagccc gtggctatcg tcgcggcagt agcctgcaaa aatcggcggc gtgaaatcat
    27541 cgctcatccc tgcaaaaaaa tcgggcagcg tcgtgctgcc cgtgtacata cttttagtcg
    27601 atggtacgca gcagttcgtt aatgccgact ttgccgcgag ttttcgcgtc aactttctta
    27661 acgataaccg cacagtaaag actgtatttg ccatctttcg acggcagatt gcctgaaaca
    27721 accacagatc ccgccggaac gcgaccgtag tggatttcgc cggtttcacg gtcgtaaata
    27781 cgggtgctct gaccaatgta tacgcccata gagatgacgg aaccttcttc gacaatcacc
    27841 ccttcaacca cttcagagcg tgcgccgatg aagcagttat cttcaataat ggtcgggtta
    27901 gcctgcagcg gctccagcac gccgccgata cccacgccac cggaaaggtg gacgttttta
    27961 ccaatctgcg cacaggaacc gacggtcgcc caggtatcaa ccatggtgcc ttcatcaaca
    28021 tatgcgccga tgttgacgta agacggcatc agcacggtgt tacgggcaat gaacgcgccc
    28081 tgacgcactg ccgctggtgg cacgacgcgg aagccttctt tctggaaacg tgcttcatcg
    28141 tagttagcga atttcatcgg cactttgtcg aagtagcggc tttctgcccc ttcgatcacc
    28201 tgattatcat taatacggaa agagagcagc accgcttttt tcaaccactg atgcgtcacc
    28261 cactgaccgt caattttttc cgctacacgc agtgcgccgg aatccagcag ggcgatcacc
    28321 tgatttaccg cttcgcgggt aacggtgtct gcattggctg gcgtgatctc ggcacggcgt
    28381 tcaaaagcgg tttcaataat gttctgtaac tgctgcattg ttaaactctt ttcatatcag
    28441 taaacacatc accctttatc gtttggattg agggcctctg tcaaccgctg atgcacttcc
    28501 tgctgcaact cgttattaag cgcacgccgg tcagcggtgg caattatgaa taaatcttct
    28561 actcgctcgc caatggttgt aattcgggcg ccatgaagcg aaattcccag atcggcaaaa
    28621 attttcccga ctcgcgccag cagtccaggt tggtcgaggg cgatcagttc gaggaacgat
    28681 ttgcggtcgg tatgggtcgg caaaaacgtc acttcggttt caacagtaaa atggcgcaat
    28741 ttggcaggtt ggcgacgcgg ctgcggtggc tgccagctac tttgtgtcag cacctgctcc
    28801 agaccaaacc gaataacctc atgacgatcg gcggacagcg ggctgccatc gggttccagt
    28861 acgatgaagg tatccatcgc cataccgtca cgagtggtga aaatttgtgc gtcgtgaaca
    28921 cttaaattgc ggcggtctaa ttcggcacag acggcggcaa acagataggg gcggtccggg
    28981 ctccagataa aaatctcggt gcctccacgc gtagcctgcg ggctaagcag taccagcggt
    29041 ttgcttaaat catgctgtaa taaatggcgt gcgtgccagg ccagttgatt tgggctatgg
    29101 cggacaaaat agttagcgcg acagcgtgac caaatttggt gcagcgcctc ttcgtcgatg
    29161 ttatccatgc gcagtagtgc cagcgcctgg agttggtgat ggcgaacccg ttcgcgcata
    29221 tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg tggcaaaata gagttcgcgc
    29281 aacagacttt gcttccagct attccacagt gtttcgttgg tggcgcaaat gtcagccact
    29341 gtcaggcata ccagatagcg cagacgattt tccgtttgaa cttcttcggc aaactgcttg
    29401 atgacttccg ggtcctgaat atcgcggcgc tgagctgtca ccgacatcag caagtgctgg
    29461 cgaaccagcc aggcgaccag ctgtgtttcg cgggagttca gcccgtggag ttcggcaaaa
    29521 tgcactacat cctgagcacc gagaatggag tggtcgccgc cgcgtccttt ggcgatatcg
    29581 tgaaacagcg cggcgatgaa aatcagctca gttgacggca ggcgcggcca gacgtccaca
    29641 cacaacggat ggcgctggcg cgtttcttca ctggcaaaac tctccagttt cagcatcacg
    29701 cggatagtat gttcatccac cgtgtaggca tggaacaggt caaactgcat ctgcccgacg
    29761 atatgcgacc attgcggcat atacgcgccg agcacgctat ggcgatgcat tggcaatagc
    29821 ccgcgccgca ccgctccggg gtgacgcaga atgctcaaaa acagttttcg cgcttccgga
    29881 atattacaca gaggttgttg caggtggcga cgggcgtggc gtaactggcg tagtgtggtg
    29941 gagtaaatgc cggtgatcgc actgttgcgc accatggtgt agaacatacg caagatggct
    30001 tccggctggc gcataaatag tgtttcatca cgcaggtcga tcagcgttcc gcgtagctga
    30061 aactcatcgt cgattggacg tggtttttcg tcggcgggaa gggcgaggat ggcttcatcg
    30121 aacagttgca gcagcatctg gttgagttca ctgacgcggc gtgtaacgcg gaagtaatcc
    30181 ttcatcatcc gctcgaccgg ttcgttacct tcaccactat aattcaaacg ctgggcgacg
    30241 ctaagctggc gatcgaataa caggcgatta tcgtaacgac tgacgaccag atgcagggca
    30301 aagcgaatgc gccacaatat atgcaggcat tcgtttaatt ccgctctctc cgcagaggtt
    30361 aagaagccaa accctaccat ttcatccagc gatgttgcgc caaaatgacg gcgggccacc
    30421 cattgcagag tgtggatatc gcgcaagccg ccggggctac ttttgatgtc tggttcaagg
    30481 ttgtagctgg tgccgtggta acgctgatgg cgctggttct gttcttcaac tttcgccgcg
    30541 tagaacttgt cggaaggcca gaatccttcg ctaaaaatat gtttttgcag ttcgaggaac
    30601 agcgcaacat cgccaattaa taagcgggat tcgattaaat tggtggcgac ggttaaatcc
    30661 gataaccctt ccagcatgca ctcttcaagc gtgcgcacgc tatgacctac ttccagcttt
    30721 acatcccaga gcagcgttaa cagctcgccc actttttgcg cctgatcgtc cgggagcttt
    30781 ttacggctta aaatcagtaa atcgatgtca gaaagtggat gcagctcgcc acgaccgtag
    30841 ccaccgacgg cgaccaatgc caggtcggca atctggctga atcccgcttc aatccataat
    30901 cgttgaagaa gctggtcgat aaactcggta cgcgcctcaa tcaactgttc tgcggagata
    30961 ccattgtcaa aggcatcacc cagccaacgc tggaaagtat cgatatgggc ttttatccca
    31021 ccgacggtta attcatcacg gggccaggcg catggatttt gcggttgacc gtgcagggtg
    31081 gggagagcgg tgtttgcgta ctgttctgga agggtattca ttgtgcgcca cccataagat
    31141 taaattattg cattaaaaaa gccggcattc gccggcttca ttttattcgt cgtgcgagat
    31201 tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt tcgcagccgt tatcagtcac
    31261 cacaatagta tgctcatatt gtgcagacaa gctgcgatct ttggttttta ccgtccagcc
    31321 atctttcatg gtgcggatct cttttttacc cgcgttgacc attggctcga tggtgaacgt
    31381 catccccggt ttcagcacga cgttggtttc acgggagtca tagtgcagca cctgcggttc
    31441 ttcgtggaag ccgcgaccaa taccgtgtcc acaatattca cgaacgacgg agaagccttc
    31501 tgcttcgaca aatttctgaa ttgccgcacc gatttcgcgc agattaatgc caggttttac
    31561 catgcgtagc gccaggtaca ggctttcttg cgtgatgcgg cacagacgtt cgcccatgat
    31621 ggtcggctta ccgacgataa acattttcga ggtatcgccg tggaaaccat ctttgattac
    31681 ggtgacatca atgttaacga tatcgccatc tttcagcagc ttggcgtcgt ccgggatacc
    31741 gtggcacacc acttcattaa tagagatgca aacggatttc ggatagccgt gatagccgag
    31801 gcaggcagaa accgcgtgtt gttcattaac aatgtaatca ttacagatgc gatccagctc
    31861 gccggtgctg acgcccggtt taacatacgg ttcgatcatc tccagcactt cggcagccag
    31921 tcggccagcg acgcgcattt tttcgatatc ttctggggtc ttgattgaga tagccattaa
    31981 ttctgtccat cagcgtcggt gataccgaca atatatatgt aagtgccgtc aatggtatca
    32041 cacccgggca aattgagaat cattctgaat ttcgccaaac gtgccactga aggttttcta
    32101 taatagaaaa ttcaacgtct gatgctgtac acagcgccaa caattattgg tgtccacgac
    32161 gtatttgtgg tataaagcgc gccggacttc cgatccattt cgtatacaca gactggacgg
    32221 aagcgacaat ctcactttgt gtaacaacac acacgtatcg gcacatattc cggggtgccc
    32281 tttggggtcg gtaatatggg atacgtggag gcataacccc aacttttata tagaggtttt
    32341 aatcatggca actgtttcca tgcgcgacat gctcaaggct ggtgttcact tcggtcacca
    32401 gacccgttac tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta acaaagttca
    32461 catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg aactgaacaa
    32521 gattgcttct cgcaaaggta aaatcctttt cgttggtact aaacgcgctg caagcgaagc
    32581 ggtgaaagac gctgctctga gctgcgacca gttcttcgtg aaccatcgct ggctgggcgg
    32641 tatgctgact aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag acctggaaac
    32701 tcagtctcag gacggtactt tcgacaagct gaccaagaaa gaagcgctga tgcgcactcg
    32761 tgagctggag aaactggaaa acagcctggg cggtatcaaa gacatgggcg gtctgccgga
    32821 cgctctgttt gtaatcgatg ctgaccacga acacattgct atcaaagagg caaacaacct
    32881 gggtattccg gtatttgcta tcgttgatac caactctgat ccggacggtg ttgacttcgt
    32941 tatcccgggt aacgacgacg caatccgtgc tgtgaccctg tacctgggcg ctgttgctgc
    33001 aactgtacgt gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa gcttcgtaga
    33061 agctgagtaa taaggcacgc tcgtacgagc ccttatagac caggtagtac acgtttggtt
    33121 agggggcctg catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca
    33181 gatcacatct ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg
    33241 cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc
    33301 gacatcgagc tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa
    33361 gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc
    33421 attctggaag ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc
    33481 gcagacaaag ttctggacgc agctgttgct ggcaaaatca ctgacgttga agtactgaaa
    33541 gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt
    33601 cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc
    33661 ggtgttctgg ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac
    33721 gttgctgcaa gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa
    33781 aaagaatacc aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca
    33841 gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag
    33901 ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa
    33961 gtgactggct tcatccgctt cgaagtgggt gaaggcatcg agaaagttga gactgacttt
    34021 gcagcagaag ttgctgcgat gtccaagcag tcttaattat cgaaaaggag ccgcctgagg
    34081 gcggcttctt tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa
    34141 agcgacgtac aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa
    34201 acatggctac caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag
    34261 ctctgcaggg cactgaaggc ttcggtattg atgcaagcat actggatcgc atggctcagg
    34321 aaatcaaaga actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc
    34381 tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg
    34441 ggatgctggc gaccgtaatg aacggcctgg caatgcgtga tgcactgcac cgcgcctatg
    34501 tgaacgctcg tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg
    34561 cagaagctat cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta
    34621 acccgttctt taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg
    34681 tggtgctgaa agcaaccaaa gttgacggcg tgtttactgc tgatccggcg aaagatccaa
    34741 ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca
    34801 tggacctggc ggccttcacg cttgctcgtg accataaatt accgattcgt gttttcaaca
    34861 tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca
    34921 cggaataatt cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggat
    34981 cttacccgta atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt
    35041 ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga
    35101 tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg
    35161 cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc
    35221 gacgccgctg cgtcagctgg caagcgtaac ggtagaggat tcccgtactc tgaaaatcaa
    35281 cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg
    35341 cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga
    35401 acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt
    35461 acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat
    35521 cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat
    35581 caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct
    35641 tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt
    35701 tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct
    35761 cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg
    35821 cgctggtggc aggcaaaaat gtcgctcgca tggtagaaca gtgcctggaa ttctctcccc
    35881 gctatgctgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac
    35941 agggtagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg
    36001 aggatgttga tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg
    36061 ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg
    36121 gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg
    36181 aacataacgc catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg
    36241 accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg
    36301 agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact
    36361 ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat
    36421 acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc
    36481 cgcagtcagt gattcactca atggtgcgct accaggacgg cagtgttctg gcgcagctgg
    36541 gtgaaccgga tatgcgtacg ccaattgctc acaccatggc atggccgaat cgcgtgaact
    36601 ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt
    36661 atgatcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc caggcagcga
    36721 cgacagcatt gaatgccgca aacgaaatca ccgtggctgc ttttcttgcg caacaaatcc
    36781 gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat atgcgcgaac
    36841 cacaatgtgt ggatgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag
    36901 aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag
    36961 cgtagggctt cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt
    37021 caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc
    37081 gtaaaccctg tcagggaata agaaacgcgt gatgttgtct gctactcaac cacttagcga
    37141 aaaattgcca gcgcatggct gtcgtcatgt tgcgatcatt atggacggca atggtcgctg
    37201 ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg
    37261 ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag
    37321 tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg
    37381 ggcgctagat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg
    37441 cgataccagt cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac
    37501 agccgggaat accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat
    37561 agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca
    37621 gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt
    37681 agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta
    37741 tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg
    37801 ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga
    37861 aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat
    37921 acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca ttgtaacgct
    37981 ggtggtctgt atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc
    38041 gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt
    38101 gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgct
    38161 gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg
    38221 gcgtaactct aaaacattgc gcattatttt tggcgtgcta accattgttc ccttcttctg
    38281 gggcatgctg gcgttacggg cctggcacta cgacgagaat cattacagtg gcgcaatatg
    38341 gctgctctat gtcatgatcc tggtatgggg cgctgactct ggcgcatata tgtttggcaa
    38401 attgtttggt aaacataaac tagcaccgaa ggtttctccg ggtaaaacct ggcaaggctt
    38461 tatcggtgga ctagtgaccg cagcggtaat ctcatggggt tatggcatgt gggtgaatct
    38521 cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct
    38581 cggcgatctg accgaaagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt
    38641 aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt
    38701 ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt
    38761 tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa
    38821 tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg
    38881 tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatcgccctg
    38941 atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt tgttccggaa
    39001 ctccgccacc atgccttcaa taataaatct gtcggccaac gagcggcgat tattgccgca
    39061 ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt
    39121 gtgcctggcg tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca
    39181 caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat
    39241 gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacggtagcg
    39301 ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag
    39361 cctgataaag aagatccggt atcttcgttg gggattcgtc ctcgtgggcc gcaaattgaa
    39421 cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc
    39481 gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt
    39541 gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg
    39601 tctttgacat taatcccgga gagtaaaccg ggtaagggta aagcgattgg ttttgtcggt
    39661 attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg
    39721 ttcaacgcta tcgtcgaagc cacggacaaa acgtggcagt tgatgaagct gacggtcagt
    39781 atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct
    39841 atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt
    39901 gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg
    39961 gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt
    40021 caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt
    40081 ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga
    40141 tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag
    40201 ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc
    40261 tcctcagtat gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca
    40321 ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata
    40381 cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca
    40441 aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg
    40501 aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca
    40561 gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg cgcaaccgtg
    40621 ttgacctaaa actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg
    40681 ttggtaacca tgctttcacc accgatgaac tgatctctca tttccaactg cgtgacgaag
    40741 tgccgtggtg gaacgtggta ggcgatcgta aataccagaa acagaaactt gcgggcgacc
    40801 ttgaaaccct gcgcagttac tatctggatc gcggttatgc ccgtttcaac atcgattcta
    40861 cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac atcaccgaag
    40921 gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg
    40981 aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca
    41041 aaatggaaga tgacattaaa aagcttcttg gtcgctatgg ttatgcctat ccgcgcgtac
    41101 agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgtgtg aacgttgatg
    41161 cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg
    41221 ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gctggggagc gatctggtcg
    41281 atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc
    41341 aacgcgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa gaacgtaaca
    41401 ccggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg
    41461 gtgtgcaaca ggataactgg ttaggtacgg gttatgctgt tggtatcaac gggaccaaaa
    41521 acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc gtagatggtg
    41581 taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg
    41641 actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata
    41701 actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg
    41761 ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata
    41821 acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc
    41881 gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattccgg
    41941 gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgattgatg
    42001 acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat ggtttaggcg
    42061 gcaaagagat gccgttctat gagaacttct atgccggtgg ttccagcacc gtgcgtggct
    42121 tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg
    42181 atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagatctg tgtaaatcgg
    42241 atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc
    42301 cgtttattag cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta
    42361 ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc
    42421 caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt
    42481 tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc
    42541 agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag
    42601 cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg
    42661 tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc aggtctcggt
    42721 ttagcactgg caacatctgc tcaggcggct gacaaaattg caatcgtcaa catgggcagc
    42781 ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa
    42841 ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg
    42901 cagtccatga aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc
    42961 cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa
    43021 gaacgcggca aactggttac tcgcatccag actgctgtga aatccgttgc caacagccag
    43081 gatatcgatc tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac
    43141 atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat
    43201 ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt
    43261 gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt
    43321 gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc
    43381 gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa
    43441 attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg
    43501 acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga atccggcgtt
    43561 gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc
    43621 ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag
    43681 aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat
    43741 cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga ttgcgtggag
    43801 atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc
    43861 gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggca
    43921 gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc
    43981 ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg
    44041 ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa
    44101 cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag
    44161 cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg
    44221 ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt
    44281 tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag
    44341 agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt
    44401 ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc
    44461 agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac
    44521 aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact
    44581 atttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga
    44641 tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc
    44701 tggtcgacgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct
    44761 gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt
    44821 cgattggcgc gaacgctcac attggtcctt tttgtatcgt tggaccccat gtcgaaattg
    44881 gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg
    44941 ataatgagat ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg
    45001 gcgaaccgac ccgcgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc
    45061 atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga
    45121 tcaatgcgca cattgcgcac gattgtacgg taggtaaccg ctgcatcctc gccaacaacg
    45181 caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag
    45241 tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc ggtgtggcgc
    45301 aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata
    45361 tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt
    45421 ataagctgat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac
    45481 tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg
    45541 gtctgattcg ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac
    45601 ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc
    45661 ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cctggtacga
    45721 aatggaagaa ctggcagtga tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact
    45781 acatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg
    45841 tattgatgcg cctgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa
    45901 aaccattcat tacgtcagtc cgtccgtctg ggcgtggcga cagaaacgcg ttttcaaaat
    45961 aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa
    46021 atacaacgta ccgtgccgct ttatcggtca taccatggct gatgccatgc cattagatcc
    46081 agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact gtctggcatt
    46141 gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttcc tgaaaacggc
    46201 ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg tgaatgccaa
    46261 acgccgcgag cagtttgaac gcatcaaagc tgcagtcgcg ccagacctgt cggttcattt
    46321 gctggatgga atgggccgtg aggcgatggt cgccagtgat gcggcgctac tggcgtcggg
    46381 tacggcagca ctggagtgta tgctggctaa atgcccgatg gtagtgggat atcgcatgaa
    46441 gcctttcacc ttctggctgg cgaagcgact ggtgaaaact gattatgtct cgctgccaaa
    46501 tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg agccgcaaaa
    46561 attggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccatg cgatgcacga
    46621 caccttccgc gagctgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc
    46681 cgttctggag ttagcacaat gatcgaattt gtctatccgc acacgcagct ggttgcgggt
    46741 gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt
    46801 gacccggcgc gcccaattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt
    46861 ctggcgctct gtgaagagat caaagagaaa gcgttgagct ggagtctggg ccgtgcggaa
    46921 cctcacgaaa tcgacgaact gaatatttta catgcgacta tgctggcgat gcagcgtgcc
    46981 gtcgctggac tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa
    47041 ttaccgatgc cttcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc
    47101 gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc
    47161 ccgcaatatg gttttgccca acacaaaggg tatccaaccg cttttcatct ggaaaaactg
    47221 gctgaacacg gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg
    47281 ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa
    47341 ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa
    47401 accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat
    47461 ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag
    47521 cccatcgtcg gggcggattt taacgtccag tgcgacctgc tgggtgatga gttaacccac
    47581 ctgacggtgc tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa
    47641 gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa
    47701 ttaaatgaag ggttgatcct tctttccggc gggcgcatgg gcgacgttgg acgcagtctt
    47761 ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg
    47821 gatcgctatt ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac
    47881 gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa cgatgtgcgc
    47941 tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt
    48001 accctcgacg atcctaaacg ccctcgtaac tattcgccgc agcaatatat gcgtagcgaa
    48061 gaggagatgt gtgagctgtt tgccgacatc cccgaagccc ttgccaacac cgttgagatt
    48121 gccaaacgtt gtaacgtaac cgtgcgtctg ggtgaatact tcctgccgca attcccgacc
    48181 ggggacatga gtaccgaaga ttatctggtc aagcgtgcaa aagaaggcct ggaagagcgt
    48241 ctggcatttt tattccctga cgaggaagaa cgtcttaagc gtcgtccgga gtatgacgaa
    48301 cgtttggata ctgaacttca ggttatcaac cagatgggct tcccgggtta cttcctcatc
    48361 gttatggaat ttatccagtg gtcgaaagat aacggtgtac cggtagggcc aggccgtggt
    48421 tctggtgcag ggtcactggt ggcctacgcg ttaaaaatca ccgatctcga tccgctggaa
    48481 tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctctatgcc tgacttcgac
    48541 gttgacttct gtatggagaa acgtgaccag gttatcgaac atgtggcgga catgtacggt
    48601 cgcgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc ggtgatccgc
    48661 gacgtaggcc gtgtattggg gcatccgtac ggatttgtcg atcgtatctc gaaactgatc
    48721 ccgcccgatc cgggtatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa
    48781 atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg
    48841 gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattact
    48901 gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa
    48961 agcgacgttg aatacgccgg gctggtgaag ttcgacttcc tcggtttgcg tacgctcacc
    49021 atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg
    49081 ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct gcaacgctcg
    49141 gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg
    49201 caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg
    49261 caatcaggga tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatctcctat
    49321 ccggacgtgc agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc
    49381 atcctgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta taccctcggt
    49441 ggcgcggata tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag
    49501 cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa
    49561 atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca ctcggcggcc
    49621 tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg
    49681 gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag
    49741 tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc
    49801 cacgtcaacg acgacggcga aatcgtgtat ggtattggcg cgatcaaagg ggtaggtgaa
    49861 ggcccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt
    49921 gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc
    49981 atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggc
    50041 gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg
    50101 ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg
    50161 tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggtctgta cctgacggga
    50221 caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa
    50281 gacatgcacc cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg
    50341 cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc
    50401 gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa
    50461 aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt
    50521 aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg
    50581 cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag
    50641 tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat
    50701 gcacgcgcgc gattgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta
    50761 aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg
    50821 aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa
    50881 atcgattctc tgaccgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa
    50941 gaagtgcatc gtttgcgtga aaaaagcgta gaactgacgc gtaaaatctt cgccgatctc
    51001 ggtgcatggc agattgcgca actggcacgc catccacagc gtccttatac cctggattac
    51061 gttcgcctgg catttgatga atttgacgaa ctggctggag accgcgcgta tgcagacgat
    51121 aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat cattggtcat
    51181 caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa
    51241 ggttaccgca aagcactgcg tctgatgcaa atggctgaac gttttaagat gcctatcatc
    51301 acctttatcg acaccccggg ggcttatccg ggcgtgggcg cagaagaacg tggtcagtct
    51361 gaagccattg cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg
    51421 gttatcggtg aaggtggttc tggtggtgcg ctggcgattg gcgtgggcga taaagtgaat
    51481 atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg
    51541 aagagcgctg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg
    51601 aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggtgc tcaccgtaac
    51661 ccggaagcga tggcggcgtc tctgaaagcg cagctgctgg cggatctggc cgatctcgac
    51721 gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggctac
    51781 gcgtaatcgg caaatgttct gaaaagggtc acttcggtgg ccctttttta tcgccacggt
    51841 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    51901 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    51961 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaagtttat
    52021 cgaacataac ccacgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52081 atgtagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52141 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52201 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52261 taacattacg ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac
    52321 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct
    52381 gttttatgat tttttcggcg ggaataccct caaggcggac gtctctattt cggtcaccga
    52441 gcttggttcg ttgctcgacc acaccgggcc acacctggaa gcggaagagt acatcgcgcg
    52501 gacgtttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    52561 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca attgtcataa
    52621 atcgctggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    52681 taatgcgttg gggattctgg gtgggatccc gcgccgtgaa tttactcgcg acagcatcga
    52741 agagaaagtc gctgccacca cgcaggcaca atggccggtt catgcggtga tcaccaactc
    52801 cacctatgat ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    52861 gattcacttc gattctgcct gggtgccgta cacccatttt catccaatct accagggtaa
    52921 aagtggtatg agcggcgagc gtgttgcagg aaaagtgatc ttcgaaacgc agtcgaccca
    52981 caaaatgctg gcggcgttat cgcaggcgtc gctgatccac attaaaggtg agtatgacga
    53041 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    53101 tgcttctgtt gagacggcgg cggcgatgtt gcgtggtaat ccgggcaaac ggctgattaa
    53161 ccgttcagta gaacgagctc tgcattttcg caaagaggtc cagcggctgc gggaagagtc
    53221 tgacggctgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgctggcc
    53281 cgttgcgcct ggcgaacagt ggcacggctt tagcgatgcg gatgccaatc acatgtttct
    53341 cgatccggtt aaagtcacta ttttgacacc ggggatggac gagcagggca atatgagcga
    53401 ggaggggatc ccggcggcgc tggtggcaaa attcctcgac gaacgtggga tcgtagtaga
    53461 gaaaaccggc ccttataacc tgctgtttct ctttagtatt ggcatcgata aaaccaaagc
    53521 aatgggatta ttgcgtgggt tgacggaatt taagcgctct tacgatctca acctgcggat
    53581 caaaaatatg ctgcccgatc tctatgcaga agatcccgat ttctaccgca atatgcgtat
    53641 tcaggatctg gcgcaaggga tccataagct gattcgtaaa cacgatcttc ccggtttgat
    53701 gttgcgggca tttgatacct tgccggagat gatcatgacg ccacatcagg catggcagag
    53761 acagattaaa ggcgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    53821 aaatatgatc ctgccttatc cacccggcgt accgctgctg atgccgggag aaatgcttac
    53881 cgaagagagc cgcacggtac tcgattttct actgatgctt tgttccgtag ggcaacatta
    53941 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggtg tttaccgcgt
    54001 acgagtccta aaaatggcag gataacttgc cagagcggct tccgggcgag taacgtgctg
    54061 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    54121 gcgacggatt atgcgatgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    54181 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatggacaa
    54241 tatgtgattg agcttttctc atttccgttc ccgccggagc gccccagccg accggaagct
    54301 tgcggcctgc gtcatctggc ttttagcgtt gatgatatcg atgcggcagt ggcgcacctt
    54361 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc cctacacgca aaaacgcttc
    54421 acgttcttta acgatccgga cgggctgccg ttggaactgt atgagcagta aggcttgtca
    54481 tcgccgcatt tgcccggtaa cgtgcccggc attgctactg taaaatcgca ccatcatgac
    54541 actcacgctc aatagacaac ttctctcctc acgccagatt ctggtggcct ttagcggcgg
    54601 ccttgattct actgtcttac tgcatcaact ggtgcagtgg cggacggaaa atccgggcgt
    54661 cactctgcgc gctatccatg tgcatcacgg tttaagcgcc aatgctgatg cctgggtgaa
    54721 acattgtgaa aacatctgcc aacagtggca ggtgccgctg gtggtcgaac gtgtgcagct
    54781 tgcgcaagag gggctaggta ttgaggccca ggcgcggcag gcacgttatc aggcatttgc
    54841 ccgcaccttg ttgcccggtg aagtgctggt tacagcgcaa catctcgacg atcaatgtga
    54901 aacctttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggcgga
    54961 agtttcggag tttgccggaa cacagcttat ccgcccgttg cttgcccgca cgcgggggga
    55021 actggtgcag tgggcgttgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    55081 tgatagctac gatcgtaact ttctgcgcct gcgcgtagtg ccgttattgc agcagcgttg
    55141 gccgcatttt gccgaagcaa cagcccgcag cgccgcactt tgcgctgaac aagagagcct
    55201 gttagatgaa ctgctggcgg atgatttagc gcactgtcaa actccgcagg ggacgctgca
    55261 gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg gcgattatcc gccgctggct
    55321 ggcagggcag aatgcaccga tgccttcccg cgacgcgttg gtgaggatct ggcaggaagt
    55381 ggcgctggcg cgagaagatg cttccccctg tttacgtttg ggtgcgtttg aaatccgccg
    55441 ctatcagtcg caactgtggt ggattaaatc cgtcactggg caaagcgaaa ccattctgcc
    55501 gtggcagacg tggctacaac cgctggaatt accggcgggg ctgggaagtg tacagcttac
    55561 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    55621 gccaggattg ctgcatattg tcgggcgcaa cggcggacgt aagctgaaga aaatctggca
    55681 agagttaggc gttccgccgt ggctacgtga caccacgcca ctgctgtttt atggcgaaac
    55741 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgtag gtgagaatgg
    55801 cgtcagtttt gtctggcaga aaacgcttag tcaagtgaaa gccggataag acgcatcaaa
    55861 cgtcgcatcc ggcgaaagtc aatcaggact cgctcaccac caccgtaccg atttccgggt
    55921 ggctaaagct ggtaatttta tccagacgca gctcgcgggt tgcaccagcg gcctcgacga
    55981 ccagatactc cacatttttg cgggagacta aatcgctggc cttcgcctgc aatttttcgc
    56041 catctttcag ctcaagtgcc agcattaaat gatgctggca ggcgagctca agattatcgt
    56101 aatcatcaca attgattggt tgatacgtat cattcattga cataatcgct caccagtaag
    56161 tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc gtcggcagcc
    56221 atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc taagtctccg
    56281 ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac atgccctcct
    56341 gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa actctgtttc
    56401 gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc acgtattccg
    56461 ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg acaatttata
    56521 aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc
    56581 tggcgcgcca tccttcagaa acccaggagc gcatgatgct gcgcctgctg gcgtggctaa
    56641 aatatgccga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat gagccggaag
    56701 cgtggctgcg taacgatcat ttgggtattg atttgtggat tgagctgggg ctgccggatg
    56761 agcgacggat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt gcctataata
    56821 gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgcgcag tttgccaatc
    56881 tttccatctg gtatctggac gatgaacaac tggcgaaagt aagcgccttt gccgatcgta
    56941 ccatgacgct gcaggcaacg attcaggatg gcgtgatctg gttatcggat gataagaata
    57001 atctggaagt gaacttaacc gcctggcaac aaccttcatg attgtgattt cccgacatgt
    57061 tgctatcccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg cgggcgggca
    57121 gcatgttaat aagacctcaa cggctattca tctgcgtttt gacattcggg cgtccagcct
    57181 gccagagtat tacaaagaac gtctgctcgc cgccagccat cacttgatca gcagtgatgg
    57241 ggtgattgtc attaaggcac aggaataccg caatcaggag ctcaaccgcg aagcggcgct
    57301 ggcccggctg gtggcagtga ttaaagattt aacaacagaa caaaaagccc gacgacccac
    57361 gcggcccacc cgcgcatcaa aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt
    57421 gaaggcgatg cgcggcaaag tgcgcagcgg tcgggaataa aaagaaggaa tggatggtga
    57481 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
    57541 atcgggccga agtcgatacg ctttctccgg cacaggctgc cgaactgaaa ccgatgccgc
    57601 aaagttggcg cggcgtgctg ccgtgtgctg attgcgaagg aatcgaaacc tctctgttcc
    57661 tcgaaaaaga cggaacctgg gtgatgaatg agcgttatct gggggctcgt gaagaacctt
    57721 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgata
    57781 gcaaaggtga aaagtcatat tatcgcgcga aaggagatgc gctggagatg ctcgatcgtg
    57841 aaggtaatcc gattgaatcg cagttcaact atacgctgga accggcacaa tccagcttac
    57901 ccatgacacc gatgaccctg cggggcatgt atttttatat ggctgatgcg gcgaccttca
    57961 ctgattgcgc gaccggaaaa cgtttcatgg tggcgaataa cgcagagctg gagcgtggct
    58021 acctggctgc gcgcggtaac agtgaaaaac cggtattact gtcagtagaa ggtcacttta
    58081 cgcttgaggc taatccggat accggtgcgc cgaccaaagt attagcgccc gatacggcag
    58141 gtaaatttta cccaaaccag gattgcagta gtttggggct gtaacccgtc ttgagacaga
    58201 aacaaatgaa atattgcctc atgcgctgcg cttatcagtc ctaaagcata caatgcaata
    58261 ttttgaatta gcacaatttt gtaggccgga taaggcgttc acgccgcatc cggcattttg
    58321 cgcacgaggc tggcttttat cactgagtca gcccaacaaa gcggcctgcc ttgatataaa
    58381 tcccaggctt catgtgccca acatactgta actgcgtttc tggcttaaac gcagagatat
    58441 cgccagtacg catatgcatc agatcggcag ggctaatcca gcctgattgc gccagcgtaa
    58501 gcggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac cacgactttt
    58561 tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg cactgttgac
    58621 gaaaatcctc ggagaacgga tttagtgagc acatctggcg agtcaccata aatggaataa
    58681 attcgacaat gccgcgatag ttgtaaccgc tatctttaat tttgttggcg aacgcggtga
    58741 tttctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc ttatcactat
    58801 gctttatggc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct tcggcaacgt
    58861 tattatctcc caaatagatt gcaacgtgac tcacagagga agtgctgaat acgcggatac
    58921 caaatgaggt taccccaagg cttgaggaga aaagcaaatc gccgggtttg agatctggtt
    58981 ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat ttaacagccc
    59041 atgttttcgc ctcggcattt accactgtgg ctgatgagtc tggctggctg atatcaacgg
    59101 tacaggccga aagtaaaaga aagcagggga gaagcaggcg gcagtacgcc tttggtttat
    59161 ccattttata caatccatgt aaaaaagggc cccgaaattc aggacccttt ctggcatcag
    59221 cctttaatct gtttcaccag ataatcgacg atgtcaccag tcttaattaa ctgtttctcg
    59281 ccgttacgac gatatttata ttcgatatca tcgttgtcga ggttacggtc gcccagcaca
    59341 atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg
    59401 cggtcatcca gcagcacttc gataccttgt gcgcgcagtt cgctgtacag tttctcagca
    59461 agttcctgta cgcggaagga tttgtgcatg ttcattggca gaatcgccac ctggaacggc
    59521 gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat cgccgcagct
    59581 accacacgcg ttacgccgat accgtagcaa cccatcgtca ggatttggtt acggccatct
    59641 tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg gaagatgtga
    59701 ccgacttcga taccacgttt gatcagcagc gtaccctgac catccgggct tggatcgcca
    59761 gccaccacgt tgcggatatc agcaatttcc ggggtagcga catcgcgatc ccagttgata
    59821 ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat cgccgcaacg
    59881 gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct
    59941 ttaaccacgg cacgaatttc ttcttcggtc gcaaaagtca gcgggctggc aacctgcggc
    60001 agtttttctg ctttaacttc gttcagctcg tggtcaccgc gcaccagcag cgcaaccagc
    60061 gggaagctac tgccttcaac ggctttaacc agcagagtct taaccgtttt ctcaatcggc
    60121 agattgaact gttcaaccag ttccgcgatg gtcttcgcgt tcggcgtatc aaccagcgtc
    60181 atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagttcaatg
    60241 ttcgctgcat agtcagaagt atcggagaag accacatcgt cttcaccgct ctgcgccagc
    60301 acctgaaatt cgtgagaggc gttaccgccg atagaaccgg tatcggcctg tacggcgcgg
    60361 aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt
    60421 tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg
    60481 gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag
    60541 aagttcagcg gcagctgttt gtaagagctc agctcgttac gaatcaggtc tgtaatcact
    60601 tcttcatgag ttgggccgag tacgaacgga cggtcaccac ggtcagcaat acgcagcaat
    60661 tccgggccat actgttccca acgaccactc tcttgccaca gttcagatgg ctgaaccacc
    60721 ggcattaaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact
    60781 tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg
    60841 atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc
    60901 ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt
    60961 agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca gtgcaaaaga
    61021 aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact tcaaaacctg
    61081 cgtcggtgac gcgccagcga acgttaaaat catgcagcca gacggcatag gttttgcccg
    61141 tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg cggataaaca
    61201 gcgttaactg cggataacgc ttcttcagcg tcaaaagctg cttttcgacc tctgcggtaa
    61261 aacacaccgc catttctgcc gctggcgcac tttgcgcata gctggcactg gcatcgggaa
    61321 gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca tcgaccagat
    61381 ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct ttcagctcta
    61441 ccagcgacat accaattggg ttagggcgaa aagtggagcg cgtagcgaaa acacccattc
    61501 tggcattacc gccaagacga ggcggacgca cagtcggacg ccagccgcct tccatcgttt
    61561 gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga acggcgtcgg
    61621 cctggttgta gggagcaatg agatgtagtt cgccgttggc gctttttacc agacctggct
    61681 ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt tgctcgaact
    61741 ggaaactgct catttcgccg tgatgttaag cgcagaaccg atacatacag cctgacgata
    61801 gcagcctggc gtaccgctgg tgacttcgca gctatgcagt aataccgcat tggctttcat
    61861 tttcgaggcg ttgatttgca tccgcttacg tgcggttgga atgctcggcg gagagtcctg
    61921 attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg gtttgccgac
    61981 taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt tcggttttgc
    62041 aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg atctgcttaa
    62101 catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac gcatagtatt
    62161 tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggcg cttgtaaaaa tgacaagacg
    62221 ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaat attaccagcc
    62281 tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcgt cagactgata
    62341 agcctgaacg aatttcttca cgttttcggc gtctttgttg tcttcacgcg ttacgatcag
    62401 gtttacgtac ggggactctt tatcttcgac aaagataccg tctttcgctg gagtcaggcc
    62461 aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt cgtccagaga
    62521 gcgcggcagc tgcggtgctt ccagttcaac aattttcaga ttttttgggt tctcaacaac
    62581 atcaagaacg gtcggcagca ggccaacgcc atctttcagt ttgatcaagc ccactttttg
    62641 cagcagcagc agtgaacgac caaggttagt tgggtcgttt ggcacggcaa cctgcgaacc
    62701 atcctgcagt tcatccagtg atttgatttt cttggagtaa cctgcaatcg gataaacaaa
    62761 agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat caaggtacgg
    62821 tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt ttggcagaac
    62881 atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg caactttctg
    62941 cgcgacttct gcaacctgct gttcggcacc aacaatcacg ccgactttaa tgtggtttgg
    63001 atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg ctcccactgc
    63061 cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt tatgttgtgt
    63121 tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact gaattaaata
    63181 aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttat agccgatgta
    63241 gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac ccatcgcgga
    63301 ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg gcaacgcttc
    63361 cggtaacagc actttacgaa cgatctgcat tggcgtggca cccattgcgc gggaggcttc
    63421 aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa taaacggtgc
    63481 tgcaccaacg gttaacggaa caatcgctgc ctgcaatcca atcgatgtac cgacaataac
    63541 gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac ggaaaatgtt
    63601 cacaatcgca gaaatggtac gatacaactt cgcgttagca ataatttgcc ccggacgcgt
    63661 gacataaagc agaacgccaa ccggcagacc aatcacaaag ccaaaaaaac cggatacgaa
    63721 ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg gctcagacat
    63781 aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg gcttgcgtat
    63841 cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa cttaacgcca ccggcgtaat
    63901 ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt tcagaaagca
    63961 gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg cagtcagtga
    64021 atggttccgc ttgcagacgc tcctgataat cttccgggat atccagatgc agggtcgact
    64081 gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt accgtgtcct
    64141 gctcgatcag ttcgccatta ctgataaccg ccacgcaatc acaaatgcgc ttcacaacgt
    64201 ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttgatg tctttaagca
    64261 gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca tcacacagca
    64321 atactttagg attgctggct aacgcacggg caatcgccac acgctgtttc tgcccaccgg
    64381 aaaggttcga cggatagcta tcatgcttat caccaagacc aaccaatgac agcaattccg
    64441 tcacgcgacg tttgatttcg tctttcggcg tgttgtccag ctccagcggc agagccacgt
    64501 tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata ccaatctggc
    64561 ggcgagcttt agtcaactca gattctgaca gcgtggtcag ctcctggcca tcaaccagca
    64621 cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta ctcttacccg
    64681 cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg ctgacgttgt
    64741 tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc gaaagtttta
    64801 tcattgatta tttattatcg tcattaagtt agtcgtggca tttcgaatgc ctggaacggg
    64861 catcgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat caggtttatg
    64921 cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcacatgc cttattaagg
    64981 agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat ggcaccatta
    65041 atgttgatca cggctatgtc catgagatcg acaactttga atttatcgac ggtgttattg
    65101 acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc aaccagtctg
    65161 gcattgctcg cggtaaattt actgaagcac agtttgaaac gctgaccgag tggatggact
    65221 ggtcgctggc ggaccgcaat gtcgatctgg atggcatcta ttattgcccg catcatccgc
    65281 agggtagtgt tgaagagttt cgccaggtct gcgactgccg caaaccacat ccggggatgt
    65341 ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg gtgggcgata
    65401 aattagaaga tatgcaggca gcggctgcgg cgagcgtggg aacaaaagtg ctggtgcgta
    65461 cgggtaaacc tattacgcct gaagcagaaa acgcggcgga ttgggtgtta aatagtctgg
    65521 cagaccttcc gcaagcgata aaaaagcagc aaaaaccggc gtgatgatta aaagatgagc
    65581 ggttgaaata aaaatgcatt tttccgcttg tcttcctgaa acgactccct ataatgcgcc
    65641 tccatcgaca cggcggatgt gaatcacttt acacaaacag ccggttcggt tgaagagaaa
    65701 aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc acctcgcgac
    65761 agtgagctga aa
//
LOCUS       DAZFRG010000002        48925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFRG010000002 DAZFRG010000000
VERSION     DAZFRG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.002"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            194..1411
                     /gene="alaA"
                     /locus_tag="ACHKUC_000062"
     CDS             194..1411
                     /gene="alaA"
                     /locus_tag="ACHKUC_000062"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO6616396.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1495..2094
                     /gene="yfbR"
                     /locus_tag="ACHKUC_000063"
     CDS             1495..2094
                     /gene="yfbR"
                     /locus_tag="ACHKUC_000063"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6616397.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2153..3985)
                     /locus_tag="ACHKUC_000064"
     CDS             complement(2153..3985)
                     /locus_tag="ACHKUC_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6616398.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4072..4722)
                     /gene="hxpA"
                     /locus_tag="ACHKUC_000065"
     CDS             complement(4072..4722)
                     /gene="hxpA"
                     /locus_tag="ACHKUC_000065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO6616399.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4733..5227)
                     /locus_tag="ACHKUC_000066"
     CDS             complement(4733..5227)
                     /locus_tag="ACHKUC_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6616400.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5310..5765)
                     /gene="yfbV"
                     /locus_tag="ACHKUC_000067"
     CDS             complement(5310..5765)
                     /gene="yfbV"
                     /locus_tag="ACHKUC_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO6616401.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYDVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6103..7305
                     /gene="ackA"
                     /locus_tag="ACHKUC_000068"
     CDS             6103..7305
                     /gene="ackA"
                     /locus_tag="ACHKUC_000068"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO6616402.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7380..9524
                     /gene="pta"
                     /locus_tag="ACHKUC_000069"
     CDS             7380..9524
                     /gene="pta"
                     /locus_tag="ACHKUC_000069"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6616403.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9714..11234
                     /gene="yfcC"
                     /locus_tag="ACHKUC_000070"
     CDS             9714..11234
                     /gene="yfcC"
                     /locus_tag="ACHKUC_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO6616404.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11267..11809)
                     /gene="yfcD"
                     /locus_tag="ACHKUC_000071"
     CDS             complement(11267..11809)
                     /gene="yfcD"
                     /locus_tag="ACHKUC_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO6616405.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11867..12418)
                     /gene="yfcE"
                     /locus_tag="ACHKUC_000072"
     CDS             complement(11867..12418)
                     /gene="yfcE"
                     /locus_tag="ACHKUC_000072"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO6616406.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12474..13118)
                     /gene="yfcF"
                     /locus_tag="ACHKUC_000073"
     CDS             complement(12474..13118)
                     /gene="yfcF"
                     /locus_tag="ACHKUC_000073"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO6616407.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13254..13901
                     /gene="yfcG"
                     /locus_tag="ACHKUC_000074"
     CDS             13254..13901
                     /gene="yfcG"
                     /locus_tag="ACHKUC_000074"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO6616408.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            13958..14320
                     /gene="folX"
                     /locus_tag="ACHKUC_000075"
     CDS             13958..14320
                     /gene="folX"
                     /locus_tag="ACHKUC_000075"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO6616409.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14341..15234
                     /locus_tag="ACHKUC_000076"
     CDS             14341..15234
                     /locus_tag="ACHKUC_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO6616410.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDKELRG
                     PFNMVSPYPVRNEQFAHTLGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15282..16172)
                     /gene="rpnB"
                     /locus_tag="ACHKUC_000077"
     CDS             complement(15282..16172)
                     /gene="rpnB"
                     /locus_tag="ACHKUC_000077"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO6616411.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDARRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMMEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16368..17141)
                     /gene="hisP"
                     /locus_tag="ACHKUC_000078"
     CDS             complement(16368..17141)
                     /gene="hisP"
                     /locus_tag="ACHKUC_000078"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO6616412.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17149..17865)
                     /locus_tag="ACHKUC_000079"
     CDS             complement(17149..17865)
                     /locus_tag="ACHKUC_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6616413.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17862..18548)
                     /gene="hisQ"
                     /locus_tag="ACHKUC_000080"
     CDS             complement(17862..18548)
                     /gene="hisQ"
                     /locus_tag="ACHKUC_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO6616414.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18638..19420)
                     /gene="hisJ"
                     /locus_tag="ACHKUC_000081"
     CDS             complement(18638..19420)
                     /gene="hisJ"
                     /locus_tag="ACHKUC_000081"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO6616415.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19641..20423)
                     /gene="argT"
                     /locus_tag="ACHKUC_000082"
     CDS             complement(19641..20423)
                     /gene="argT"
                     /locus_tag="ACHKUC_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO6616416.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALNELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20689..21258)
                     /gene="ubiX"
                     /locus_tag="ACHKUC_000083"
     CDS             complement(20689..21258)
                     /gene="ubiX"
                     /locus_tag="ACHKUC_000083"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO6616417.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(21353..22870)
                     /gene="purF"
                     /locus_tag="ACHKUC_000084"
     CDS             complement(21353..22870)
                     /gene="purF"
                     /locus_tag="ACHKUC_000084"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO6616418.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22907..23395)
                     /gene="cvpA"
                     /locus_tag="ACHKUC_000085"
     CDS             complement(22907..23395)
                     /gene="cvpA"
                     /locus_tag="ACHKUC_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO6616419.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23927..24589)
                     /gene="dedD"
                     /locus_tag="ACHKUC_000086"
     CDS             complement(23927..24589)
                     /gene="dedD"
                     /locus_tag="ACHKUC_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO6616420.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24579..25847)
                     /gene="folC"
                     /locus_tag="ACHKUC_000087"
     CDS             complement(24579..25847)
                     /gene="folC"
                     /locus_tag="ACHKUC_000087"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO6616421.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEMIDARRSGGK"
     gene            complement(25917..26831)
                     /gene="accD"
                     /locus_tag="ACHKUC_000088"
     CDS             complement(25917..26831)
                     /gene="accD"
                     /locus_tag="ACHKUC_000088"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO6616422.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26987..27646)
                     /locus_tag="ACHKUC_000089"
     CDS             complement(26987..27646)
                     /locus_tag="ACHKUC_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6616423.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27729..28541)
                     /gene="truA"
                     /locus_tag="ACHKUC_000090"
     CDS             complement(27729..28541)
                     /gene="truA"
                     /locus_tag="ACHKUC_000090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO6616424.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28541..29554)
                     /locus_tag="ACHKUC_000091"
     CDS             complement(28541..29554)
                     /locus_tag="ACHKUC_000091"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6616425.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFILTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGSPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29620..30756)
                     /gene="pdxB"
                     /locus_tag="ACHKUC_000092"
     CDS             complement(29620..30756)
                     /gene="pdxB"
                     /locus_tag="ACHKUC_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO6616426.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LNELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30855..31850
                     /gene="flk"
                     /locus_tag="ACHKUC_000093"
     CDS             30855..31850
                     /gene="flk"
                     /locus_tag="ACHKUC_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO6616427.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31847..33025)
                     /locus_tag="ACHKUC_000094"
     CDS             complement(31847..33025)
                     /locus_tag="ACHKUC_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6616428.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATMFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33290..34510)
                     /gene="fabB"
                     /locus_tag="ACHKUC_000095"
     CDS             complement(33290..34510)
                     /gene="fabB"
                     /locus_tag="ACHKUC_000095"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO6616429.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34669..36675
                     /gene="mnmC"
                     /locus_tag="ACHKUC_000096"
     CDS             34669..36675
                     /gene="mnmC"
                     /locus_tag="ACHKUC_000096"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO6616430.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSKLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIHYQHQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36796..37074)
                     /locus_tag="ACHKUC_000097"
     CDS             complement(36796..37074)
                     /locus_tag="ACHKUC_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO6616431.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(37108..37656)
                     /gene="epmC"
                     /locus_tag="ACHKUC_000098"
     CDS             complement(37108..37656)
                     /gene="epmC"
                     /locus_tag="ACHKUC_000098"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO6616432.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37656..38465)
                     /locus_tag="ACHKUC_000099"
     CDS             complement(37656..38465)
                     /locus_tag="ACHKUC_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6616433.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPVLVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38465..39289)
                     /gene="mepA"
                     /locus_tag="ACHKUC_000100"
     CDS             complement(38465..39289)
                     /gene="mepA"
                     /locus_tag="ACHKUC_000100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO6616434.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39293..40378)
                     /gene="aroC"
                     /locus_tag="ACHKUC_000101"
     CDS             complement(39293..40378)
                     /gene="aroC"
                     /locus_tag="ACHKUC_000101"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO6616435.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRRESDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40413..41345)
                     /gene="prmB"
                     /locus_tag="ACHKUC_000102"
     CDS             complement(40413..41345)
                     /gene="prmB"
                     /locus_tag="ACHKUC_000102"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6616436.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAVREHFAIYKD"
     gene            41511..42062
                     /gene="smrB"
                     /locus_tag="ACHKUC_000103"
     CDS             41511..42062
                     /gene="smrB"
                     /locus_tag="ACHKUC_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO6616437.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            complement(42382..43239)
                     /locus_tag="ACHKUC_000104"
     CDS             complement(42382..43239)
                     /locus_tag="ACHKUC_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO6616438.1"
                     /translation="MKITNLLFRLLILMVFMLGSRAGMGQTFRNVYIYYATFGWNSQV
                     ELYLDVAEVSSVYYGTYYQSNYVMTLGNLPNRTWTGTGSSPAPTITVKDGTTVNNSFC
                     PGMEALEAKDSRWECLKLPLDITVDGDVGGCPWLVSIRATSQVVVGSVDKDIYYGPNA
                     TVSVCPTVSVTPYDVSWDENYINKTKTLSLQSTGGVIEKTLSTYLMKDGKLCDGSQMN
                     EEGAYCRWVAQMITFSSSGCDNANVTVTPNRHPITDKQLHDMVVHVDTSSRQPIDSTC
                     RFQYTLNEL"
     gene            complement(43241..43768)
                     /locus_tag="ACHKUC_000105"
     CDS             complement(43241..43768)
                     /locus_tag="ACHKUC_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6616439.1"
                     /translation="MNLKRISHCAAALATVLLTPHVQAADNLHFYGNLLSKSCTLVVQ
                     GESLADVHFPTVSRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDASSGAQGVGIGMEKTDGTPVPINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGEFTASLTATFEYI"
     gene            complement(43765..44244)
                     /locus_tag="ACHKUC_000106"
     CDS             complement(43765..44244)
                     /locus_tag="ACHKUC_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6616440.1"
                     /translation="MKRLAWCLIYGFAGLVQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSDGTFSAYATLQVDYQ"
     gene            complement(44241..44744)
                     /locus_tag="ACHKUC_000107"
     CDS             complement(44241..44744)
                     /locus_tag="ACHKUC_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6616441.1"
                     /translation="MKAFFLTGSAALLLACGVTVFPAMAGGKIVNLTLRVLVDGPPPC
                     SVKGSVVEFGNVIIKSIDGANYRQPVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAVDHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            complement(44761..45513)
                     /locus_tag="ACHKUC_000108"
     CDS             complement(44761..45513)
                     /locus_tag="ACHKUC_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6616442.1"
                     /translation="MSYKLSCPMLVSTALMALLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HSLPSDRESLFYYNVREIPPKTGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(45533..48181)
                     /locus_tag="ACHKUC_000109"
     CDS             complement(45533..48181)
                     /locus_tag="ACHKUC_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6616443.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKHWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNAGEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            complement(48262..48828)
                     /locus_tag="ACHKUC_000110"
     CDS             complement(48262..48828)
                     /locus_tag="ACHKUC_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6616444.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT        11829 a        13287 c        12281 g        11528 t
ORIGIN      
        1 acataacatt acttattaag catatgcacc tcattatttt gccattatta agttattaac
       61 agcacaatcg agccttcccc tctggcaaaa tcttattctg cagaccttca aaacaccgtc
      121 ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat aacagcaaaa
      181 aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg tctgttatga
      241 catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta acaaggtact
      301 gaaactgaac atcggcaacc ctgccccgtt cggttttgac gcgccagatg aaattctcgt
      361 tgacgtgata cgcaacctgc ctaccgctca agggtattgc gattccaaag gtctttactc
      421 cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg ttaccgtgga
      481 agatatttac atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc aggcattgct
      541 gaacagcggg gacgaaatgt tggttcctgc acctgattac ccactatgga ccgcagcggt
      601 ttcgctctcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg actggttccc
      661 ggacctcgat gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg ttattatcaa
      721 cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga ttgtggagat
      781 tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa ttctctacga
      841 cgacgctgag catcactcca ttgcgccgct ggcacctgac ctgctgacca ttacctttaa
      901 cggactgtcg aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg tgttgaacgg
      961 gccgaaaaaa cacgccaaag gttacatcga aggtctggaa atgctggctt caatgcgcct
     1021 gtgtgctaac gttcctgcgc aacacgccat tcagaccgca ctgggtggtt atcagagcat
     1081 cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg cgtgggaatt
     1141 gatcaacgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt atatgttccc
     1201 gaaaatcgac gccaaacgct ttaacattca cgacgatcag aaaatggtgc tggatttcct
     1261 gttgcaggaa aaagtcctgt tggtgcaagg gacggcattc aactggccgt ggccggatca
     1321 cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga gcaagttcgc
     1381 gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga gattgcatcc
     1441 ggcagtgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt cactatgaaa
     1501 cagagccatt tttttgccca tctctcccgc ctgaaactca ttaaccgctg gccgctaatg
     1561 cgcaacgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat ggtcgcccat
     1621 gcgctggcag ctatcaaaaa tcgtaaattt ggcggtaatg tcaacgccga acgtatcgct
     1681 ttactggcga tgtaccacga tgcttcagaa gtgctcaccg gagatctccc tactccggtg
     1741 aaatacttca attcgcaaat cgctcaggaa tacaaggcta ttgaaaaaat cgctcagcaa
     1801 aaactggtcg atatggttcc ggaagagctg caggatatct ttgcgccgtt aattgacgag
     1861 catgcatata gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact gtgtgcatat
     1921 ctgaaatgtc tggaagagct cgcggccgga aataatgaat tcttgctggc aaaaacgcga
     1981 ctggaagcga cgcttgaagc gcgccgcagc caggagatgg actacttcat ggaagtattt
     2041 gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct gtaagcagcc
     2101 ggagtctgcg tcgcatcagg caataagcac cggatgcgac atcaggctct tgtcaaaacg
     2161 gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg aacggtaccc
     2221 ccaacttaac aaagtcactg aagctgtaat tccccggacc taaaaccagt gtgttaaccg
     2281 gtgaagaaac cggcgtcata aaggcggcgg atgctgccat cgccacgacc atcgcaaatg
     2341 gataaggcga cacacccatc gttttggcag cagccagcgc aatcggagcc atcaacaccg
     2401 ccgtcgcggt attagaaata aatagcccaa taaccgccga caagacaaac aaacaaccca
     2461 gcatcatatg tggcccataa ccgccgccaa tgtccatcag ccctttcacc gccagcgcga
     2521 cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc aaaataatgc
     2581 tcggccagtg aatggattta taggcacttt cggcatcgat acagcggaat ttccccatca
     2641 gcaggcaggc gatgatagcg gcaactggat taggaatttc atcggtcagc attaacgcca
     2701 ccattagcac cagacagaaa atcgcatggg gtgcctggct gtgcgcgggc gatgcttcac
     2761 tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgtttggcc agcataccga
     2821 tcagtttcca gttacccaca accaggatga tatcgcccag cagcagaggc tcatccgcca
     2881 gcgaaccttc cagcgccacg ccattgcgct ttagccccac tacattcaat ccgtagcggg
     2941 tacgaaaacc aatttcgcgc accgatttac caataagttc tgactcagga attaatgaaa
     3001 tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc agtaccatcg
     3061 gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca gacatatcaa
     3121 taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg ataacgcgac
     3181 gaaaacgccg ccagcgttca acaccgatga cgttagcacc ataacgttca cgtaatttga
     3241 gatcgtccag tcgttgacca atcattggcg atccggggcg aatagccaga cgtcgcgcac
     3301 gcccggtcag tcgatattca cggataagat cgcgaaaggt tcggcgcgtc cagccttcgc
     3361 gttgtggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata tacaaaatac
     3421 ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag ccgtgatagc
     3481 cttcacgcag caattcactg ttgactacca ggttcggcgg tgtcgccacc agcgtcatca
     3541 tgccgcttat gagcccggca aaacttaacg gcatcatcag acgcgacggc gacgtttgca
     3601 tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgccg gttgaactca
     3661 taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg atttcactgt
     3721 tgcccgccac tttgaccagc catgtcccca ttacagtggc aacaccggta cggaccaaac
     3781 catcgccaat aataaacaag gcggcaatca ggacaacgtt aggatcggaa aagccggaaa
     3841 atacttcggg gaccgtcagc gttccgctta atgcaaacgc gacaataaca aacaaagcga
     3901 ccgcatccat acgcactctg cccgtcgcaa acaagacgat ggcaacggcc agtaatgaaa
     3961 gaacccaaat caattcaccg ttcacaactt atccttgtta attgaggggg ataacttgat
     4021 tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt ttcactgaat
     4081 aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt ggaggactaa
     4141 atcgacttca ttcaggcgcg gggtatctgc cggggcatta accgcaataa catggcaacc
     4201 cgccgccaga cctgaaagca cgccagcggg agcatcttcc accacaacac actcctgcgg
     4261 cgcaagcccc agcagctgcg cgcctaacag atacgcatca ggttctggtt ttccgcgctt
     4321 cacccgctca gcggttacaa acacctctgg cgcgggaagc cccgctattt tatgacgcgc
     4381 tcgcgctacc ggcatggagc cagaagtcac aatagcccac ggaatacctg ctttattcaa
     4441 atgattgagt aaggcgattg cccccggaag cgcggtaata ccttcggttt ccgtggcctc
     4501 gatgtgctcc agacgcgtaa actcggcggc aatgtctgcc tcggatttgc ccgccataaa
     4561 atggcgcaga gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcga
     4621 taacccatga cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac
     4681 cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcgt
     4741 taataatttg attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaga
     4801 cattaagcat gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccgtgagttc
     4861 cagcgtcaaa atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt
     4921 aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt
     4981 gctggtcctg caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga
     5041 tgatagtgcg gcaggtttct tctttcagct cgccaaactc gcgatccagt tcacgcatct
     5101 gtaatccata accacgctca atgattgttt gcaggcgacg gtaacgttca gcatttgccg
     5161 gatcgagcat agtcatcatc ttgtactggt tagacaaaat aagacgttgc gcgttggtca
     5221 tttccattat tgactcctgt atcactctac tacggtgaaa aaaaagaagg ctgagtatgc
     5281 cttcttttat atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt
     5341 tgtttgaagg cacgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg
     5401 ggtgccaaca cctgtccaga ctcctgcaat ttaccgcgaa cgtcataaaa ccagttgagg
     5461 attgcaggag gtaatggcgt tacagaacgc ttgcccagcc accacaatcc ctgcatgggt
     5521 aaacttaagg cgaacagcgc agtggcaact gccggcccaa gctgaccgcc cagagcaatc
     5581 tgccagcaga gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc
     5641 ttgataacgc gattttcgac aaagactggg gcaaggcgtt tttccagcgg ccacgtcttt
     5701 gagtaatgct gtccccggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt
     5761 gacatgcttc acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc
     5821 agcgggacaa cgttcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag
     5881 cctgaaggcc taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt
     5941 ggctgaaaat tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa
     6001 tttgcagtgc atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct
     6061 ccctgacgtt tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta
     6121 ctggttctga actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt
     6181 gaagagtacc tttctggttt agccgaatgt ttccacctgc ccgaagcacg tatcaaatgg
     6241 aaaatggacg gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg
     6301 ctcaacttta tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact
     6361 gctatcggtc accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat
     6421 gagtctgtta ttcagggtat caaagatgca gcttcttttg caccgctgca caacccggct
     6481 cacctgatcg gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt
     6541 gctgtatttg acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg
     6601 ccgtacaacc tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac
     6661 ttctatgtaa cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc
     6721 atcacctgcc acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt
     6781 gacacctcta tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat
     6841 atcgatccgg cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc
     6901 aacaaactgc tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc
     6961 cgctatgttg aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac
     7021 tgccaccgcc tggccaaata catcggcgcc tacactgcgc tgatggatgg tcgtctggac
     7081 gctgttgtat tcaccggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg
     7141 ggcaaactgg gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc
     7201 ggcaaatctg gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac
     7261 gaagaactgg ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc
     7321 tcagctggcg gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg
     7381 tgtcccgtat tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc
     7441 ttggcgtgat ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg
     7501 ctcagccgcg taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact
     7561 cttccaccac gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca
     7621 gcaatcagaa agatgtgctg atggaagaga tcatcgcgaa ctaccacgct aacaccaaag
     7681 acgctgaagt cgttctggtg gaaggtctgg tcccgacacg taagcaccag tttgcccagt
     7741 ctctgaacta cgaaatcgcc aaaacgctga acgcagaaat cgtcttcgtt atgtctcagg
     7801 gaactgatac tccggaacag ttgaaagagc gtatcgaact gactcgcaac agcttcggcg
     7861 gtgcaaaaaa caccaatatt accggcgtta tcgttaacaa actgaacgct ccggttgatg
     7921 agcagggtcg tacccgtccg gatctgtccg agatttttga cgactccacc aaagcaaaag
     7981 tgaacaacgt tgatccggcg aagctgcaag aatccagccc gctgccagtt ctcggcgctg
     8041 tgccgtggag ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg
     8101 cgaccatcat caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg
     8161 cacgcagcat tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg
     8221 cagaccgccc tgacgtgctg gttgccgctt gcctggctgc catgaacggc gtagaaatcg
     8281 gtgccctgct gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac
     8341 gtgctttcgc taccggcctg ccggtattta tggtgaacac caacacctgg cagacttctc
     8401 ttagcctgca gagcttcaac ctggaagttc cggttgacga tcatgagcgt atcgaaaaag
     8461 ttcaggaata cgttgctaac tacatcaacg ctgactggat cgattctctg actgccactt
     8521 ctgagcgcag ccgtcgtctg tctccgccag cgttccgtta tcagctgact gaacttgcgc
     8581 gcaaagcggg caaacgtatc gttctgccgg aaggtgacga accgcgtacc gttaaagcag
     8641 ccgctatctg tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccagcagaga
     8701 tcaaccgtgt tgcagcctct cagggtgtag aactgggtgc aggcattgaa atcgttgatc
     8761 cagaagtggt tcgcgaaaac tatgttggtc gtctggtcga actgcgtaag aacaaaggca
     8821 tgaccgaaac cgttgcccgc gaacagctgg aagacaacgt ggttctcggt acgctgatgc
     8881 tggaacaaga tgaagttgat ggtctggttt ccggtgctgt tcacaccacc gcaaacacca
     8941 tccgtccgcc gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt
     9001 tcttcatgct gttgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc
     9061 cgaccgcaga acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg
     9121 gtatcgaacc gcgcgttgct atgctctcct actccaccgg tacttctggt gctggtagcg
     9181 acgtagaaaa agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gatctgatga
     9241 tcgacggtcc gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcac
     9301 cgaactctcc ggttgcaggt cgcgctaccg tgttcatctt cccggatctg aacaccggta
     9361 acaccaccta caaagcggta cagcgttctg ctgacctgat ctctatcgga ccgatgctgc
     9421 agggtatgcg caagccggtt aacgacctgt cccgtggcgc actggttgat gatatcgtct
     9481 acaccatcgc gctgactgcg attcagtctg cacagcagca gtaatctcgt catcatccgc
     9541 agctttgcgc tgcggatatc tgaaccggaa atagtcacta tttccggttt tttattctat
     9601 taatttgcat taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc
     9661 catctcactt tgcatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg
     9721 caatcactga atctaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct
     9781 tttttgttgc cattttaacc agccttgcca cctgggtagt tccggtgggc atgtttgaca
     9841 gtcaggaagt gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac
     9901 actcatttcg cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt
     9961 tcacgacggg cgatgaacgc ccgggtctga tgaacttccc gtttgaaggg ttaacctcag
    10021 gatcgaaata cgggacagcc gttggcatca tcatgtttat gctggtgatt ggcggcgcgt
    10081 ttggcattgt gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata
    10141 cccgcggaaa tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg
    10201 caatatttgg tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg
    10261 tccggctggg ctatgacagt attaccaccg tccttgtgac ctatattgcc acgcaaatcg
    10321 gttttgccag ttcgtggatg aacccgtttt gtgtggttgt tgctcagggt attgccggcg
    10381 ttccggtgct ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actctgattg
    10441 gcctgatctt taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg
    10501 tgcatgagtc cgaccgcttc tttcgtgaaa agcaggctga tgttgaacaa cgtccgttta
    10561 cctttggtga ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gttatttggg
    10621 gcgtgatcgt taatgcctgg tttattcctg aaattgccag ccagttcttc accatgggtc
    10681 tggtgattgg catcatcggc gttgttttcc gccttaacgg catgacggtt aataccatgg
    10741 cttcatcctt taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg
    10801 ccaaagggat tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata
    10861 ccatcctcaa cagcattgcc aatgccatta gcggtctgga taacgcagtc gcggcctggt
    10921 ttatgttgct cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg
    10981 cgttaaccat gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta
    11041 ccgtgctggc gttccagttt ggtgatggct tcagccacat catttaccca acctcagctt
    11101 cgttaatggc gacgctcggt gtttgccggg tggacttccg taactggctg aaggtgggtg
    11161 cgaccctgct tggactgctg tttattatgt ccagcgtcgt ggtgatcggc gctcagttga
    11221 tgggctacca ctaaaaatgt taagagccgc gattgcggct cttttttcat tctgccgttt
    11281 cagtctctac ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg
    11341 agtctggagt gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt
    11401 cactgacttc atcttcctgt agagcgaagg gaccgtgaga gacgcagctg aacaacgcgc
    11461 cccagacacg gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac
    11521 cggcaatgcc caactcttct tccgcttcgc gacgcgcgga ttccagcagc tgctcatcgg
    11581 cctggactac accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg
    11641 tacgacgctg gaccagaatt ttgcccatgc catcatgcac gacgatataa gttgcacgat
    11701 gacgcagaca ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt
    11761 cattgacaat atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac
    11821 cttctttttc tggcgctctt acggcgcatt ttgagttgtg ggtaaattac ggattaatcg
    11881 cgacctgcgc aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca
    11941 gcatgccata actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt
    12001 ggaaaatctc cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat
    12061 cgttctggtt taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca
    12121 gacgttgttt ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca
    12181 tttgatccac ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat
    12241 taagccgttc agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccat
    12301 gattcaacac gtcgccaagg atcaccagcc actgggcacc gctttggaca aacaactcca
    12361 gaacacgttc cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca
    12421 ctcactcctc gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca
    12481 gattgcttcg ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc
    12541 gcataatcca ccaggcgttc cggcacctca tcgccatgta gtaccaggcg gttaatcatt
    12601 agcgccagat cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct
    12661 aacaaatgtt ctgccatcgc gaacagtttc tctgcactgg cttttccctc ggccgttagt
    12721 ggcgcttttt tcgcccccgc aaagacaaca tccgtcgggc gttcttcgcg gatgggcatc
    12781 agatcgctgc gcagccaggc ctgaatctgt cgtgctcgcg cacgattttc taaatcaagc
    12841 ggataaatac gttcccaggt cggtggcgca aatcgatctt ccagatactc cgcaatggca
    12901 gaagattcac tcaactcaaa atcatcgatt tgtaataacg gcacacggcg tgtctgaccg
    12961 taaccttgcc acgtcggctg caaatgttca ccgctgtcga ggtcgatggt cttgatatga
    13021 aacgacaggc ctttctcctg caacgccacc caggcggata acacataagg ggagaaaaaa
    13081 tgggcatctg accaaagcgt gatagcgggt ttactcatta tgtcctcggc aataaagggg
    13141 gcttgctatc aaactataga gcctttttgt cactgtcacg tgacgaatac tcacgcaagt
    13201 cgccgcgctt tctatacttg tggtgttcag atgattagac aaccggagct gcaatgatcg
    13261 atctctattt cgccccgaca cccaatggtc acaaaattac gctgtttctc gaagaagcag
    13321 ggctggatta tcgcttaata aaggtagacc tggggaaagg aggtcagttt cgcccggaat
    13381 ttttgcgcat ttcgcctaac aacaaaattc cggcaattgt tgatcattct ccagccgatg
    13441 gcggcgaacc gctaagcctc tttgagtctg gtgccatttt gttgtatctg gctgagaaaa
    13501 cgggactctt tttgagtcat gaaacgcgtg aacgcgccgt cacattacag tggttattct
    13561 ggcaggtagg cggactgggg ccgatgcttg ggcaaaatca tcattttaat cacgcagccc
    13621 cccaaaccat tccttacgct attgaacgtt atcaggttga aactcagcgt ctgtaccatg
    13681 tactgaacaa gcggctggaa aactcgccct ggctgggtgg cgagaactac agcattgcgg
    13741 atattgcctg ctggccgtgg gttaatgcct ggactcgcca gcgaattgac ctcgcaatgt
    13801 atccggcagt caagaactgg catgagcgga tccgttcgcg ccctgccacc gggcaggcac
    13861 tgctaaaagc acaacacggt gatgagcgtt cggatagtta acagaaacag gttctcgtgt
    13921 attatttcat tctaagtaaa acaacggaga acctgcaatg gcacaacctg ccgctattat
    13981 tcgtataaag aaccttcgtt tgcgtacgtt tatcggaatt aaggaagaag aaattaacaa
    14041 ccgtcaggat attgttatca atgtgactat ccactacccc gccgataaag cgcgcactag
    14101 cgaagatatc aacgatgcgc tgaattatcg caccgtaacg aaaaacatta ttcagcatgt
    14161 agagaataac cgtttctctt tgctggaaaa attaactcag gatgtgctcg atatcgcacg
    14221 tgaacatcac tgggtgacgt atgctgaagt ggagatcgat aaactgcacg cgctgcgcta
    14281 cgccgattcg gtatccatga ccttaagctg gcagcgttaa ccgccatatc gggaggctgc
    14341 atgaacatag tgatcaccgg agggacggga ttaattggtc gccatttgat tccacgtttg
    14401 ctggacctgg gccatcaaat cacggtagtg acgcgtaacc cgcagaaagc cagttccgtt
    14461 ctcggccctc gggtgacact atggcaaggg cttgccgaac aaagcaacct caacggcgtt
    14521 gatgcggtaa tcaacctggc cggagaaccg attgctgata aacgctggac tcacgagcaa
    14581 aaagagcgtc tctgccaaag ccgctggaat atcacgcaaa aactggtcga tttgattaat
    14641 gccagcgaca cgccgccgtc ggtactcatt tccggctcgg caacgggcta ttatggcgac
    14701 ttaggtgaag tggtggttac cgaagaggaa ccgccgcata acgaatttac ccataaactc
    14761 tgcgcccgct gggaagagat tgcctgtcgg gcgcaaagtg acaaaacgcg agtgtgcctg
    14821 ctgcgtaccg gcgtagtgct ggcaccagac ggtggtatcc tcggtaaaat gctgccgcca
    14881 tttcgttttg gcctgggcgg gccgattggc tctggtcggc agtatctggc ctggattcat
    14941 attgatgata tggtcaacgg tattctctgg ctgctggata aagagctgcg cgggccattt
    15001 aatatggttt cgccctaccc ggtgcgcaat gaacaatttg cccatacact gggtcatgcg
    15061 ctgcatcgcc cggccatttt gcgcgtccct gcgaccgcca ttcggttgtt aatgggcgaa
    15121 tcttcagttc tggtattagg cggtcaacgc gcgctgccaa aaaagctgga agaagcgggt
    15181 tttgcgtttc gctggtacga tttagaagag gcgctggcgg atgtcgttcg ctgatgtggt
    15241 ttactgcaaa catccgccag ttaactcccg gtgttacagg gttagtggct ttgcgcgata
    15301 agatcgtctg gtgaaagtcg ggtcagcgtc aggatgactt cgtggtcaaa tcctttttcc
    15361 atcatctcct gagcaatgcg cagggcttct tcgtgtttgc cctgcattgc gccttcttca
    15421 cgtaatctgt cagcaatggt catcagtttc tccttttctt gtggtgcgcg ttccgctatc
    15481 tcaccaataa atgcacggaa acgccgggca tcccccgttt gtaatacgta attaaacagg
    15541 gcttttagct gtctgtcatt agtgttccct gtaactagca gcgaaacaat ttggtcgact
    15601 aatcccaaca gatcgcgctg acgaatatgt ttctgaatta actccaacag cgccattttg
    15661 cgatgttgca taatctcgtc atccggcacc acggtaatat ccaccagcgg aaaagccgat
    15721 gaataaattt tgcgggcaat cgcaggctcg gcaaattcat caagccagca gagtgaatat
    15781 ggataagggc ttctgcaacc atgataaaac agcatcggga tcactaatgg aagctcttta
    15841 taacccgcat caagatggtt ttgcatcgcc gcaatggaat agcgcatcat gcgaaaagcc
    15901 attaattcct ctggcttact ttggtgctct atcaccacat aaatataacc cgctccctcc
    15961 tgcgttttca cagaccacaa gaggtcggaa taatattgcc gcaggtcttc atcaataaaa
    16021 ctgtttggtt ccagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg cgcgggaaga
    16081 tgaatatcaa taaaatcccg cgcggtgtct ggatggcgta aaaaagattt aaataccgca
    16141 tcatgcggcg tggaagttgt cgatattgtc atggcgttcc gtcacccctc aaatttaaga
    16201 tgacgcgaca ataaccacga tttacaccca atgcagcagg cagtttttat ctttggacag
    16261 cgaattcaga actattttta gcggactgca acttttacat ttcagcggaa gcggactcgc
    16321 aaattcccca tgaaatactc cggcagcgtt aaccaccgga gaggaaatta tttcagcgaa
    16381 cctttaagga actgttgcag acgagggctt tgcgggttac cgaataactg ctccggcgcc
    16441 ccttcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac atgacgagca
    16501 aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag ttgctgcata
    16561 atacgcaaca cttcgcctac cagttcagga tcgagcgctg aagtaggttc atcaaacagt
    16621 aaaacttccg gttccatcgc tagcgcccgc gcgatagaaa cacgctgttg ctgaccaccg
    16681 gacaggtgta ccgggtattt cccctgcgca cgttcgtcta tcccgacttt tgccagatat
    16741 ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat cggcgcttcc
    16801 atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa taccatcgtc
    16861 aggcgtgtgc gcaataagcg cagctgattt ttatcggcga ctttgagttg accgtcttta
    16921 tcgcgcacca gattgatcgt ctggccattg accacgatcg atccttcgct cggtttttcg
    16981 aggaagttaa tgcagcgcag aaaggtactt ttacccgatc ccgacgatcc gatgatgctt
    17041 atgacatctc cggcattcgc ttgcaatgag acccctttca gcacttcgtg ttcgccgtag
    17101 cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc agtgcgttga
    17161 agaaggtttc acatgctgca accagcgttt ttcagctcta cgaaagaggc tgatcaggac
    17221 ataagagatg attaaataga gcaccgcggc aatgccgaaa gcggtaaaag gttgatatgt
    17281 ggcggcgtta atatcgcggg caattttcag cagatccggc accgtggcag taaatgccag
    17341 cgcagtagag tgcagcatca ggatcacttc gttgctgtac gccggtaacg caatacgcag
    17401 cgcagaaggc aaaataatgc agcgatacat tttaaaggtc gagaagccat aggctctggc
    17461 ggcttcaatt tccccatgcg gcactgaacg gattgccccg gcaaaaatct cggtagtgta
    17521 agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc agaccactgc gaaaaaaggc
    17581 gttaaggaac tcggttccct taacaatctc cagcgtgtac atgccggaat agaacaccag
    17641 caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga ttggaaactg
    17701 gatgtattta ttacttgaga cgcgaccaat cgccagaaac agcgccagaa ctccgcctat
    17761 cactaccgac aaaatcagca gccacagggt gatcgccacg ccagtaaagc gataaccgtc
    17821 agtccacagc agtggtttcc agtattcatg taagatttcg atcacaggtc agccctcttc
    17881 acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac ggtggtgaaa
    17941 acaagataaa tcacgccaca gacgatagcg aaatagaacg gttcccaggt acttttgcct
    18001 gccagttgcg tggctttgac cacatcttcc aggccgagta acgaaaccag tgcggtagat
    18061 ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat catcgccgga
    18121 aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc cgcctctata
    18181 tgtcctttcg gcactgccat aaacgcgcca cgaaacgttt cggtaaaata agcaccatag
    18241 ataaaaccga gagtgataat acctgcgacc atcggatcaa tatcaatctg cccgacgccc
    18301 atcgcctccg tcaccgtgtt tagcgcaatc tgcaaaccgt agaaaatcag cagcatcaat
    18361 actaaatccg gcacgcctcg aatcagcgtg gtgtaccctt cgaaaataag gcccgaaagc
    18421 cgattttgtg agagcttacc gccagcgcca attaaaccga tgattacagc gagcactaca
    18481 gagctgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc tgaaaaccca
    18541 tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt
    18601 tacccgaagg gagcgggctt tctttcaccg atggcgatta accaccataa acatcaaaat
    18661 cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt
    18721 tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg gtccctacgc
    18781 caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta ccgacgggtt
    18841 gtttgaggaa accttcgctg gctgcgacct catcctggaa cgcggcatca atacgtccgg
    18901 cagtcaggtc agaataaata ttgtcctgcc cttgataaga aacgatttca atgccttttg
    18961 gtgcccagtg ttcgttaccg aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt
    19021 tgcctttcag cgattcgact gtcggctgaa tgtcggaatt tttcgccacc accagacgag
    19081 aatcggcagc gtacagtttg tcggtgaagg ctatttcttg ctgacgtttt tccgtaatgg
    19141 aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt aacgcatcga
    19201 gtgggttttc gacaaacgta cattgcgtat tgatgcgttt gcacaactcc ttcgccagat
    19261 cgatatcgaa gccaaccagt tcaccttgtg aattttttga ttcaaatggc gcataggtcg
    19321 gatcggtacc gatgcggatg ttttgcggaa tcgcagcaaa tgccgcagtt gcgctggaga
    19381 aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca actgacagtc
    19441 tttttatcgg gaatatttac aggtttgtta cacttatcgt gccataaaat tgaccatcaa
    19501 ggcaaaatat cccgccccgc cagacgtatt atttttcatt aaaaatgctt aagtatgcat
    19561 ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca ttccatcagg
    19621 tacagcttcc ctgcgacgta tcagtcaccg tagacattaa agtcgaaata ctttttcgcc
    19681 atcttgtcgt aggtgccgtc ctgacgcagc tcgttaagcg ccttattgaa ggccgccgtc
    19741 agttcagcat catctttacg cagccctaca ccggtgccgt caccgaagta ttttttgtct
    19801 ttcactgatg ggcctgcaaa ggcgaaatct ttaccggcgg gttgcttaag gaaaccttcg
    19861 ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag atcggaatag
    19921 actaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca ggtctcgtta
    19981 gcgtatgctt cctgggttga tccctgcagc acaccaacat gtttaccttt cagtgaatcc
    20041 agcgttggct gaatcggtga acctttggcg gcaatcaaac gagaatctgc ggcgtacagc
    20101 ttgtcagaga aggctatctc ctgctgacgt ttatcggtaa tggagagcga cgaaataatg
    20161 gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcgct ggcaacccag
    20221 gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc aaagccaaca
    20281 aagtcacctt tggcatcttt cgatgagaac ggtgcgtagg tggtatcggt tccgatacgt
    20341 accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac taacaaagac
    20401 agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt tttatgttgt
    20461 gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct tgacgacagc
    20521 agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtccta aagttgaaga
    20581 tagcaggtat agcgattgaa tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag
    20641 gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcgaagtt atgcgccctg
    20701 ccagcgggca aagagatctt caggaagggt taccgcaaac tggtcaagaa cacgattaac
    20761 cgtctgattt atcacatcat caagggattg tgggcgatga taaaacgccg gaacgggagg
    20821 cataatcacc gcaccgattt ctgccgcctg agtcattaaa cgcagatggc ctaagtgcag
    20881 tggcgtttca cgcacgcaga gcaccaacgg gcgacgttct ttcagcacca catctgccgc
    20941 acgggtcagt aagccatcag tatagctatg gacaatgccg gacagggttt tgattgaaca
    21001 gggtaaaatc accatcccca acgtctggaa agaaccggaa gaaatgctgg cagcaatatc
    21061 gcgcgcatcg tgtgtcacat cagctaacgc ctgcacttcg cgcagggaaa aatccgtttc
    21121 gagggataag gtctggcgcg ccgcctggct cattaccaga tgcgtttcga tatctgtgac
    21181 atcgcgcaga acctgtaata aacgcacgcc ataaatcgcg ccgctggcac cgctgatgcc
    21241 tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac tttgcaggat
    21301 ttcggcggga gttgcaagtc agggtgccag accggcaccc tgagtgaagg catcatcctt
    21361 cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg gcgtcgtcat
    21421 tacgtaaagt atcgaggaaa tcgaggtagc cctgatcaac gtctttggtg acgtagacgc
    21481 cgttgaacac cgagcattca aactgctgga tatccgggtt ttcagcgcga acggcttcga
    21541 tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat
    21601 ctacttcgcg accgtgagcg atcagttccg tggcgctcgg catatcgata ccgtaaacgt
    21661 tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct ccggcttcgc
    21721 gtgccatctc gataatctgc tcagaagtgg tgccacggac gatagagtcg tcgaccagca
    21781 ggacgttttt atcgcggaac tcggcgcggt tggcgttcag tttacggcgc acggacttac
    21841 gacgcagctg ctgacccggc atgatgaagg tgcggccaac ataacggttt ttaacgaagc
    21901 cctggcggta cggtttgccc agaatacgcg caatttccag cgcgatatca cacgaggttt
    21961 ccggaatagg gatcaccacg tcgatatcca gatcttccca ttcgcgggca attttttcgc
    22021 ccagtttggt gcccatattc acgcgcgcgc tgtaaacgga aattttgtcg atgaacgagt
    22081 ccgggcgggc aaagtataca tactcaaaca ggcacggatt gctgaccgga ttgtcagcac
    22141 actgacgggt aaataactgc ccttcttcag tgatgtaaat cgcttcgccc ggcgcgacgt
    22201 cacgcaggaa atcaaagccc agcgtatcga gcgcaacgct ttcggaagcg accatatatt
    22261 ctgtgcggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc ccgttaggat
    22321 cgcggaaagc aaccataccg tggccgatga tcatcgccac acaggcatac gcaccgcgga
    22381 ttaagcggtt tgtagcagcg atggcggcga aaatgttgtc ggcttccagc gggtagtggc
    22441 ggaagttgtc cagctcgctg gcgaagatat taagcagaat ttccgagtcg gaagtggtgt
    22501 tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat
    22561 tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc tgcgcttcag
    22621 aggcgctgga gctgccagcc gtggggtaac gcacatgacc aatgcccata ttgccctgca
    22681 aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct ttccgcaaac
    22741 ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg cgatgctgaa
    22801 gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg ataccgacaa
    22861 taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac gctctgggca
    22921 agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa ctgaactgcg
    22981 ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa
    23041 agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag acgcccagca
    23101 cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg aagttcacga
    23161 tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaatccca tttcgaacca
    23221 gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga ctggcaacaa
    23281 agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg cggatcaggc
    23341 taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag accatatgtg
    23401 tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg
    23461 tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa atattcaacg
    23521 ccatcgactt tttatgcctt tgcggcatcg ggcaatgcgt gtcggatgcc gcatgaacgc
    23581 cttatccgac ctacagttct gctccagcac aggcctgata agacgcgctg gcgtcacatc
    23641 aggcaacggc tgtcggatgc agcgtgaacg ccttatccga cctactgttc tactcctgcg
    23701 taggcctgat aagacgcgct agcgtcgcat caggcaacgg ctgtcggatg cagcgtgaac
    23761 gccttatccg acctactgtt ctacccctgc gtaggcctga taagacacgc cagtgtcgca
    23821 tcaggcaacg actgtcggat gcggcgtgaa cgccttatcc gacctacggt tctacccctg
    23881 cacaggcctg ataagatgcg ccagcgtcgc atcaggcaag accgtattaa ttcggtgtat
    23941 agcccattac cacgccactt aagccagaaa gctgcttgag ctcacccagc gatcctttca
    24001 gcttctcttt ggaagcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg
    24061 tggatggcga cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca
    24121 ctttatcggc atttttcagc gcgcccagtt gcacaacata agctttaccc gttggcgctg
    24181 ctttttcttc cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg
    24241 gcttaagcgg ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa
    24301 actcggtgtt attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg
    24361 cccgtacctc ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg
    24421 gcatcatatc aggctcatcg cgatcgcccg cttttggcac cagcgggata gccgcgaact
    24481 catcctgata atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg
    24541 ctaccagcac gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc
    24601 gcgtcaatca tttccatgac atgtgcgacc gtgtggaacg aaccacacac cagcacggtg
    24661 tcttccgctt tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat
    24721 ttgccgttac ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt
    24781 ggcgcacaat accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct
    24841 ttatcatgta gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata
    24901 cgcccggtga gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc
    24961 gactcgctca caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg
    25021 gcattttcac tgacctccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc
    25081 ggttgcggga caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgtc
    25141 cagtcatgat cggtgacgga atagttccac tcaacgcccc gacgttgtag cagtgcacct
    25201 ttttcctgcg ccacatcagc aatggtagaa ggcatctccg gctcaccgac aattgccggt
    25261 ttttcgctgc gaaagatacc tgctttctcg cgaccaatac tttcacgatc tggccccagc
    25321 cagtcggtat gatccagcgc aatactggtt actaccgcga catcggcatc gacaatattg
    25381 gttgcgtcca gacgaccgcc cagccctact tccagaatca ctacgtcaag ttgtgcctgc
    25441 ttgaacagcc acaacgccga cagcgtaccg tattcgaaat aggtcaggga aatatcaccg
    25501 cgtgccgatt caatctccgc aaaagaagcg gtgtgggccg attccggcaa ttcctgaccc
    25561 tgcacacgta cgcgctcggt ataacgcacc agatgcggcg aactgtagac gcccactttg
    25621 taccctgccg ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta
    25681 cccgcaacgg taaacacaaa tggcgctggt ttcagaacgc caagacgcgc cgcgacctgg
    25741 ctcacgcgct caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc
    25801 cacgaagcca gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat
    25861 tcgttacggt gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag
    25921 gcctcaggtt cctgatccgg taccggtggc accactacgc cttcacgcgg cgcttcagga
    25981 ttcggcgctg gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc
    26041 ggacgacgga cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat
    26101 cctggcggca gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccaatt
    26161 aacgctttcg gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg
    26221 cccatggtcg ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc
    26281 agtgccgcag aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg
    26341 ccaccagagg cggagaagca gatcagtggg cagttatctt ccagcgcctg ctcaacggca
    26401 cgcacgaaac gcgcacctac cacagatccc atggaaccgc ccataaaggc gaactcgaat
    26461 gccgcagcga caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt
    26521 tcgccggttt ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac
    26581 ttcagcacat ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac
    26641 aggctatgca ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc
    26701 tcaagattac gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc
    26761 cacacccctt caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca
    26821 atccagctca ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg
    26881 gacgcataat gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca
    26941 gcccgaaact ttggataaaa aagtggtcga accgcagagt tactttttat tttgcggcgc
    27001 gtgccgctgc gcgtttgtgg cgaataaatt cgaaaacacc tggcaatatt gaaacaaaaa
    27061 taatgccaac aatcatcagt ttaaggttgc tctgaatgaa agggagcgtg ccgaagaaat
    27121 agcctgcgta ggtaaaaagc agtacccaca acagtgcgcc aatcacgtta taagcggcga
    27181 aatgacggta cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg
    27241 gcacaaaacg ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt
    27301 tgtcgagata actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga
    27361 acaaccgccc gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca
    27421 ccaccatcat atggacgttg agatcgttgg tctccagtga tgccaacgct ccggcaacaa
    27481 acagcaatga atcacccggt aaaaacggcg ttaccaccag accggtttca cagaacaaaa
    27541 ttaagaacaa aatggcataa acccagacgc cgtactccgc gaccagctct gccagatgca
    27601 cgtcaatgtg caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct
    27661 tgtactcgtc atctatcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat
    27721 tattctcgtt agtccgccag aaatagcggg cccattggcg gttttggaag atcatatcgg
    27781 tcagggtaat ccaccgcgac cagatatagc ccttccgctt ttgccgttgc tgccgccagc
    27841 gttctgtcct ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg
    27901 acttccatca ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg
    27961 atatctacca ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac
    28021 ggggtccggg actgacactg caccgcacgg aacgaggtga aatcattctc gcccagcaaa
    28081 cattgcgcag cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct
    28141 ttactcaata ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc
    28201 gtggcgctaa atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg
    28261 tcaccaggta aattcgcatt tacgcctaac gtccacgcag cgtctttgcg ctgcgcggtc
    28321 gtttcgaaat gcaccacctg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag
    28381 aagacggtga tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca
    28441 ctgcggactt cattctgccg ttgccagccg taatacctac tgccgtcgta ctcaatgccc
    28501 agcgcaattt tataaactgg cggttgttgc tggtcggaca ttagtacaga tactcctgca
    28561 ccagtttctc ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca
    28621 ccgaccagaa ctggacttgc tccggcatac cgtagtcatt acgtacgcag ccaacagaaa
    28681 gatgcggact accagaagca tctcctacct gagtcgggaa ttcgttctct tcagagagca
    28741 caatatcttc gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg
    28801 cttcaaagtt gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg
    28861 aaatcatcag cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt
    28921 cacgcacgct accttcgcta tccggcagta acggcagcat gttgaacgcc agctgacgcc
    28981 cgaagaaatc ttcttcgtca atcggaatgc cattgagtaa tttagcactc tgccccgcta
    29041 atgcatcgac cgctttttta ccctgggcgg aggctgaaat caggctggta acgctgatac
    29101 gtgataaacc gccctgatca attagcggtt tcagtgccgc cagcagctgg ctggtcagac
    29161 tgtctggtac ggcgatgaca ttccggttgc ggtaatcggt cagtataaac gggtttactt
    29221 ccggcaccac cagcggtacg tcgggttcga gagcaaacaa tccgctgctg tcgatcacca
    29281 gacaacctga gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa
    29341 aaaatgccag ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgtct
    29401 taccaccaaa gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc
    29461 caaccgggaa ctgacgttca gccagcgttt caagcagggc ttcgcccaca gcgccagttg
    29521 cgcccaggac ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag
    29581 ctccctgcca atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac
    29641 ggcgttaaaa cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata
    29701 cagagatgac cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg
    29761 tatcccggcg actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac
    29821 cagccttttc agcgttggct gatcgagcgg gccatgcagc gtaatgcgac caaactctgg
    29881 cgcgggcagt aatgtatcca gtgcaacgtg ctgttcatgc ccaataaact tgctataagc
    29941 ttcaaacact tgcgtagtac cgcgtgcttt accttccagg gtatagcctg cgatatgcgg
    30001 cgtgccgata tccacttttt tcagcaattc cacgttaagt tccggttcgc cttcccagac
    30061 atccagcact acgcttaact tctggccttc gttcaggcag gtcagcaggg cggtattatc
    30121 gacgactgcg ccacggcagg cgttaatcag aatcgctccc ggtttcaggc tgcggatcag
    30181 tttttcatcc gccagatgca gcgttttgta cggaccatct ttaaagagtg gcgtatggaa
    30241 agtcagaata tccgcatgct ggactaactc attcagcgag cggaaatccc cctcatcccc
    30301 acggtcggcg cgaggcggat cgcaaagcaa ggttgtaatc cctaacgctt ccagtcgcgc
    30361 ctgtaaacga cgaccaacgt taccaacgcc aacgatcccc acggtacgct cgtgcagtga
    30421 aaatccatcg cgttcggcaa gcatcagcag ggaggagaaa acatattcca ctaccgcaat
    30481 cgcattacag ccaggtgcag cggaaaaacc aattcccgcc tgctttaacc atgcttcatc
    30541 gacatggtcg gtccccgcgg tggcagtgcc aacaaattta atgggttttc ctgccagcaa
    30601 agattcattc actttcgtga ccgaacgcac cataagcgca tccgcgtctg ccagttgagc
    30661 gacgggaatt ggacgcccgg gaaccgcggt cacctcaccc aaacggctaa ataagtcgcg
    30721 ggcataaggc atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga
    30781 gagttaacca gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg
    30841 tataaggata cgtaatgata caacctattt ccggccctcc tcctgggcaa ccaccaggtc
    30901 agggagataa cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt
    30961 cgctggaaag cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt
    31021 gggcgggtat caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact
    31081 tccctgccgc tgagcataat ctggcgcaac gtctgttggc cgcgcagaaa agccattctg
    31141 cccgccagct tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg
    31201 tcacggatta tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc
    31261 aattaaaaac cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc
    31321 agcgcgaggc gaccgaccgt cctttattac cggcggagca caatgcgctc aaacagctgg
    31381 tgaccaaact tgcggcagca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg
    31441 aactttccgg ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga
    31501 cctggctaca ggcgcgtcag acgctaagcc agcaaaatac gccgacgctt gaatcactac
    31561 agatggcgct gaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc
    31621 agcaaaaata tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc
    31681 ttaaccagct ttatcaacgg cgggttaaag ggattgagcc gcgtgatatg caaccgctgc
    31741 ttaatccttt tccaccgatg atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt
    31801 ggatactgtt agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa
    31861 acgacagtat cgtgacaata attcccagca ccgcagagat cgccccggca agaaataccg
    31921 aagagtaacc aaacatggtc gccagcattc ccgcaagcgg cccggagacg ccgagggcga
    31981 tatcctgaaa cgcggcgtaa ccgcccagtg cggtgccgcg aacgtgtgag gggacgcgtt
    32041 taaccacctc cacgcccagc gcaggaaaga taagcgaaca tccggctcca gttaacgccg
    32101 cgcccgctaa tgcgacccac gcccctgggg cttgccagag cagcaacaag cccaccgttt
    32161 ctacaagcag agagacgatc gccactttca cgccgccaaa acggtccggc atccagccaa
    32221 acatgacgcg catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg
    32281 cccatccttt gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc
    32341 cttgtagtgc cagacctaac cctggtttcc agataagccc gacaacgctc cacagcgatg
    32401 gacgttcccc cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta
    32461 agggtaatgc cattgtggtg agcgccagtg cggcaaaacc gtaatggcta tgaatcaaca
    32521 ggccaagcgg cgcaccaaca gcgagggcac cgtaaatagc cattccgttc catgacatca
    32581 ctttgccgga atgttttggc ccaacgatgc ctaatcccca ggtcagagcg cccgtcagta
    32641 actggctttc accaaagcca agaatcaaac gcccgacgac caacagagca aatttgaacg
    32701 gtgcggggac gggcaaaatc gccgccagca gcaacgcgcc ccccgccaga ccacaagcta
    32761 acatcccctg aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac
    32821 cacgcgtcag caccgtagcc agaaactgaa tcccgacggc aatgccgacc atagtgttgc
    32881 catagcccag ttcatgatga acaaacagcg ggataaccgg caacggtaaa cctacggtca
    32941 tgtaggtgag aaaaaccgca aaagcgatgc ggaagagcga aaaattggca gaagatcgtg
    33001 tttcggtttg gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta
    33061 tccccgtctc atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg
    33121 tcttttctgc aggaaatcga caaagcggga agtttgcctg gaactggcgg cgattgtcaa
    33181 tgatgtgaaa aagggaacca tcgggttccc ttttgcgtta gtgccggagg cagcattggc
    33241 gcgtaacgtc ggatgcggcg ctggcgcgtc ttatccgacc tactccgaat taatctttca
    33301 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataactg
    33361 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca
    33421 gctcttcaat gttgatgctc ggggcgataa aaccgtgttc cagcatcagc agagagtaga
    33481 tagcttcctg tacgccagca gcgcccagtg agtgaccggt cattgctttg gttgcagaaa
    33541 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagttctttc acgtcgccaa
    33601 ccggagtcga agtaccatga gagttcaggt agtcgattgg ggtatcaaca ccgtgcatcg
    33661 ccatcttcat gcagcgcact gcaccttcgc cagacggagc aaccatgtct gcgccgtcag
    33721 aggttgcgcc gtagccaacg atttcagcat agatgtgagc accacgcgcc agcgcgtgtt
    33781 ccagctcttc aaccactacc ataccgccac cgccagcgat aacgaaaccg tcacggtgag
    33841 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    33901 tcgcgtcgaa ttcgcaagcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    33961 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    34021 cacacgcgga gctgatggag tagttaacgc cgtgaatttt aaacggcgtg gcgaggcagg
    34081 cagaaacgcc ggatgccatc gctttggtga ccacatacgg gccaaccgct ttcaggccgc
    34141 gcgggccgcg cattgcgtca gcgccgaaca cctgaaaacg cggggaaccg ccgccggaac
    34201 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggggagagg ccagcatctg
    34261 caattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    34321 ctttgcggtc aatgaggcca gtggtatcca gttttacgtt gccccagacg tggctacgca
    34381 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    34441 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    34501 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    34561 gtacacttgt acgccgaaca agtccgatca gccaattaat agagaaattt gcgcagcctg
    34621 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    34681 gccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    34741 gacgatgtct atttttccaa cgataacgga ctggaagaga cgcgttatgt ttttctcggg
    34801 ggtaaccatt tagaggcacg ttttcctgaa catccacatc ctctgtttgt ggtagcagag
    34861 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc
    34921 gaagcacatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    34981 ctcacccgtg cggatttagc gctggcgcat caacactggc cggagctggc cccgtgggcg
    35041 gaacagcttc aggcgcaatg gcctttgccc ttgcccggtt gccatcgttt attgctcgat
    35101 gaaggccgcg tgacgctgga tttatggttt ggcgatatta atgaactgac cagcaaactg
    35161 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagcgaaa
    35221 aacccggata tgtggacaca aaatctgttt aacgccatgg cgcgactggc acgtccgggc
    35281 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggaagccgga
    35341 ttcactatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg
    35401 gaacagacat tacccctccc ctgctccacg ccgtggttta accgtacggg cagcaacaaa
    35461 caagaagcgg caattattgg aggcggtatt gccagcgcgt tgttgtcgct ggcgctattg
    35521 cgtcgcggct ggcaggtaac gctttattgc gcggatgagg ctcccgcact gggtgcttcc
    35581 ggcaatcgcc agggggcgct gtatccgctt ttaagtaaac acgatgaagc gctaaaccgt
    35641 tttttctcta atgcgtttac ttttgctcgt cggttttacg atttattgcc cgttaaattt
    35701 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    35761 gcacagatgt tgtcgatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    35821 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggta ttacttatcc gcaaggtggc
    35881 tggctgtgtc cggcagaact gacccgtaat gtgctgaaac tggcgcaaca gcagggtttg
    35941 cagattcatt atcaacatca gttacaggat ttatcccgta aggatgactg ttggttgttg
    36001 aattttgcag gagatcagca agcaacacac agtgtagtgg tactggcgaa cgggcatcaa
    36061 atcagccgtt tcagccaaac gtcatctctg ccagtatatt cggttgccgg gcaggtcagc
    36121 catattccga caacgccgga gctggcgaaa ctgaagcaag tgctgtgcta tgacggttat
    36181 ctcacgccgc aaaatccggc gaatcagcat cattgtattg gtgccagtta tcaccgtggt
    36241 agcgaagaga cggcgtacag tgatgaagat cagcagcaga atcgccagcg gttgattgat
    36301 tgtttcccgc aggtacagtg ggcaaaagag gttgatgtca gtggtaaaga ggcgcgctgc
    36361 ggtgtgcgtt gtgctactcg cgatcatctg ccaatggtag gcaacgtgcc tgattatgac
    36421 gctacactcg tggaatatgc gtcgctggcg gagaagaaag atgaagcggt aagcgcgccg
    36481 gtttatgacg atctctttat gcttgctgct ttgggctcgc gcggtttgtg ttctgccccg
    36541 ctgtgtgccg agattctggc agcacagatg agtgaagaac cgattccgat ggatgccagt
    36601 acgctggcgg cgttaaatcc gaataggtta tgggtgcgga agttgttgaa aggtaaagcg
    36661 gttaaggcgg ggtaatctgc tttggcattg tttgtcggat gcggcgtaaa cgccttatcc
    36721 gacctacgcg ttacctgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    36781 tccagacgtc acaacttact gctgtgaggc ctgctgaaac aggtgttccc acatatcggt
    36841 taccagcgcc tggtcacgcg gtgacaattc acccgcgcca atggcttttt ccagactctg
    36901 gctaacggtt gtatgtaccg cctgagcgga gtggtcgtca ccactttcca gttctgcgat
    36961 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    37021 gtcaaccata ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    37081 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    37141 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccggacg
    37201 ttcgggaatg ccgtttgcca gataatccat cacctgcgca tgtacccgcc gttgaaacac
    37261 cacgcggtcc ggttcgaaat ccccttccag attgtcgcag ctaacattaa acggatatcc
    37321 cgctgccacg cagaacaacc agtccagcgc ctgcggcttc acttcaacat cttcaaactg
    37381 gctttgtgtc tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    37441 acggcgcgct ttcccggcaa tacaccagtg cgaaatctcg tggatggcgc tggcataaaa
    37501 gccatgagcg aagacgattc tgttatacgg tacttccgca tcagcaggaa gatagatcgg
    37561 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    37621 ctcaatcaac tgctcatagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    37681 atctcctgtc catggctatc gtaaagtaat ttggcactca tcaccgccga gacgataacg
    37741 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca gtcttgaacc catgcgcgcg
    37801 ccgaggaact gccccaccag catcacaaag cccgtcgccc aaatcacttt gccgccgaga
    37861 ataaacagca gcagaccgcc aatgtttgac gttgcgttaa gtaatttggc gtgagcagtg
    37921 gctttggcga ggttgaatcc gcacagcgta acgaaggcca gcgcgtaaaa cgatccggca
    37981 gccgggccaa aaaatccatc gtaaaaaccg acgcagcccc ccgccaccag cgcaaatggc
    38041 agtccataca tccgacgctg gcgatcttct tcccccagct ttggcatcag caagaaatag
    38101 agaccgatac aaatgaccag gacaggcaaa atctgccgta agacatcggc ctgaacatac
    38161 tgcaccagta gcgcgccgct cattgagccg acaaaggtca tggcgatatt gagtttttga
    38221 tcgcttaaac taaccacttt acggcgaata aagtagatag tggcggaaat agagccaccg
    38281 caggcttgca gtttattggt tgccagcgcg ttagcgggag acatccccgc cgccatcaat
    38341 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaaccc cgccagcatg
    38401 gcgacaaaaa agagaactcc cagcagcagc ggggaaacca taaacaggct attaaacgtt
    38461 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    38521 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    38581 ccccgcaacc atcgcctggt ggcggtaaag gttgatcctc acactccaga ctatcggcag
    38641 gacaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca
    38701 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    38761 aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    38821 tccacagcgc ggggacaacg tgtttaccgt cgcgggatac taagtccagt gcttgcgggc
    38881 gcaagagctg cgcggaggtc cagcgtgttt ttggcagttg caggaagata tcgacatcca
    38941 gtccggtctg gtggctggcg tgaccgccgt tgaaacgacc accagcaggc atccccatat
    39001 cgccaatcag caccgtaccc atgcccagat tgctcacctg gcgactcaga cgctggataa
    39061 acatcaccag atccggatgt ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    39121 gttcggactg tatcggcaac gtgtcagcac cgacgataca gccattagaa aaactgccta
    39181 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    39241 tggcgctact ggcaagcagg gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    39301 gtggaatatc agtcttcaca tcagcatttt gcgcccgttg ccgtaacagg tgatccatta
    39361 aaacgatcgc cagcatcgct tccgcgatcg gcaccgcgcg gatcccaaca caaggatcgt
    39421 gacgaccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatggtg cgccccggca
    39481 cggtaatgct ggaggttggc ttcagcgcca tatgggcaat gatttgctgc ccgctgctga
    39541 taccgccaag aatgccgccc gcatggttgc tctggaaacc gtctttggtg atttcgtcgc
    39601 ggttctggct gccacgcagc gctaccacgt caaaaccatc accaatttcc acacctttca
    39661 ccgcgttaat gctcattagc gcatgggcga tgtcggcatc caggcgatca aagaccggct
    39721 cgccaagtcc ggcgggtacg ccactggcaa cgacggtgac tttcgcgccg atggagtcgc
    39781 cctctttttt cagcgcgcgc atcagttcat ctaaggcgtc gattttgtcc gggtccgggc
    39841 agaaaaacgg attttgctcg acctgagacc agtctttgat ttccagtgga atgtcgccca
    39901 tctgggtcag gcaaccgcga atctcaatgc caaatttctc ggcgagatat tttttggcaa
    39961 tcgcccctgc cgccacgcgc atagcggttt cacgggcgga agaacgtcca ccgccgcgat
    40021 aatcgcgcag gccgtatttt tgttcatagg tgtaatcggc atgacctgga cggaaaacgt
    40081 ccttaatcgc actataatcc tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc
    40141 tggtgccagt agtaacacct tcaaaaacac cggagagaat tttgacctga tccgactcgc
    40201 ggcgttgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt
    40261 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    40321 cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    40381 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatgttca
    40441 cggaccgcca gcagctgctc tttggtgagc ataaacacac catcgccgcc gttatcaaac
    40501 tccagccagg tgaacggaac atccggatat tgttccataa gatgtaccat gctgttgccg
    40561 acttcacaaa tcagcacgcc atcatcagcg aggtaatctg ccgcgttacc gagaatgcga
    40621 cgcgtcagtt tcaggccgtc ggtgccagat gccagaccca gttccggctc gtggcggtat
    40681 tcgtttggca ggtcggacat atcttctgca tcgacgtacg gcgggttggt gacgatcagg
    40741 tcgtactgca ctttcggcaa gtcacggaac aagtcggaac gaatcggaat gacgttgtgg
    40801 atcaaaccgt gttcttcgat gttctgttca gcaaccgcca gcgcgtctgg agagatgtcc
    40861 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    40921 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    40981 ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca
    41041 tggccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    41101 cggatcacgc gctcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcatg
    41161 tcttccggaa tatccagcgg caggtagagc gaaggcaaca ccagttgcac ggcttcatcc
    41221 cacgggttat cggtgccgtg gccgtaccag atattcgccg cgctgaagcg gctcaccgac
    41281 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    41341 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    41401 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    41461 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagttaa atgaaaaaga
    41521 aaacaacact tagcgaggag gaccaggcgc tgtttcgcca gttgatggcg gggactcgca
    41581 agattaagca ggacacgatt gtccatcgac cacaacgcaa gaaaatcagc gaagtaccgg
    41641 tcaagcggtt gattcaggag caggccgatg ccagccatta tttctctgac gagtttcaac
    41701 cattattaaa tactgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgaag
    41761 cgaagaaact gcggcgcggt gattattccc cggagttgtt tcttgatttg cacggtctga
    41821 cgcagctaca agccaaacag gaactggggg cgttgattgc cgcctgccgc cgtgaacatg
    41881 tgttttgcgc ctgtgtgatg catggtcatg gtaagcatat tttaaagcag caaacgccac
    41941 tgtggctggc gcaacatccc catgtaatgg catttcacca ggcaccaaaa gagtatggtg
    42001 gtgatgcggc attgctggtg ttgatagaag tagatgagtg gctgccgccg gaattgccct
    42061 gaatatttaa aggtggacga gtctgtccat ttagccttga atactggcgt ctcaaccctg
    42121 gcgataatga acgtaataat cgggcattaa ttgaaaaacg taagcagctt tccgacagcc
    42181 ttctgtgggc taatggcatt gccccttcag aggggcttga ggatgacgac ctgctgcaag
    42241 cgcttatcga cgaattactc accctcgtcg acggttatct ggcacctttc gctcccgtga
    42301 tggcgaatat tctaaaaaac tggatgaccg catgacataa cggacaacgg ggcctgatgg
    42361 ccccgttgtg tggtgtatac gctacagctc gttgagcgtg tactggaagc ggcaggtgga
    42421 atcaatgggc tggcggctgc tggtatcaac atgcaccacc atgtcgtgca gttgtttgtc
    42481 agtaatggga tgtctgtttg gcgtcaccgt aacattagca ttatcgcagc ctgacgatga
    42541 aaacgtgatc atttgcgcga cccagcgaca gtaagcgccc tcttcattca tttgcgagcc
    42601 atcacatagt ttgccgtctt tcatcagata ggttgaaagc gttttttcaa ttactccgcc
    42661 cgtcgattgc aaagacagtg ttttcgtttt attgatatag ttttcatccc atgaaacatc
    42721 atacggcgta actgacacgg ttgggcacac ggacacggtt gcatttggtc cataatagat
    42781 atctttatca acagaaccga ccacaacctg gctggtggct ctgatactca ctaaccacgg
    42841 gcagcctccc acatcaccat cgacggtaat atccagaggc agcttgagac attcccagcg
    42901 gctgtctttg gcttccagtg cctccatgcc cggacaaaat gaattattta cagttgtccc
    42961 atcttttacc gtaatcgtcg gcgcaggaga actgcccgtt cctgtccatg tgcgattggg
    43021 taagttgcca agcgtcataa cataattact ttgataatag gtaccgtagt aaacactcga
    43081 aacctcagca acatcaagat acagttcgac ctgactattc cagccaaacg tagcatagta
    43141 gatatacaca ttacgaaatg tctgccccat cccggcccga ctaccgagca taaaaaccat
    43201 caggatcaaa agacgaaaca gcaagttagt tattttcatc ttaaatgtac tcaaacgttg
    43261 cggttaaaga agcggtaaat tcacccatcg tcacttcccg gccgcttttc gcctggagcc
    43321 aggcattaaa attcagattg ttattgccgt tactcagcac aaaggtcgcg ccgctggtat
    43381 tgttaatcgg caccggtgtt ccatcggttt tttccatgcc aatccccacg ccctgcgccc
    43441 ctgaggacgc atccagcgcc agaaagccgg gctgttcact gtcttctgta ccggtcagcg
    43501 tcacccgaac ctcatactgg gctggccctt tacaatcttt caactgaaac actaccggca
    43561 cccgcgccga ctgcccggtc accatcaggt ctttgcggct aacggtgggg aaatgcacgt
    43621 ccgccagcga ctcgccctga acgactaacg tgcaggactt gctgagtaaa ttgccgtaga
    43681 aatgcaggtt gtcagccgcc tgaacatggg gcgtcagcag caccgtcgcc agtgcagccg
    43741 cgcagtgaga tatccgtttt aggttcattg gtaatccacc tgtaatgttg cgtaggcaga
    43801 aaaggttccg tcgctgagcg ccgcatcatt ggctttcacc ggcacagctt gcagcgttgg
    43861 cggcgttgac gggtcaattt tcaacggtgt tcccagctta aacggctggc cgttttgctg
    43921 tagctcaatc cccaggccgc taacgtccgt ttcaatggcg gaatcatcat aaggggtttg
    43981 gctgcccgtc caggtcaggc tcatctccca ggcgtcgtcg cgcacatcgg ggtcacatgt
    44041 gatggtgtaa ggcacatcct gacggaaatt atcgccgtta atgttgtcga taatgacgtt
    44101 gcgaaactca acttcgatcg tcttaccatc gctgatagta caggcgggcg gcgaaaccag
    44161 cgtgccgtga aaagtaacgt cgttaatcgc ggcctgcacc aggccagcaa aaccgtaaat
    44221 taagcaccat gcgagtcgtt tcattggtag tccaccacca tggtcatggt cgcgttaaac
    44281 tccgatgcca tcagttccac accgctctgc ttcaccggaa cggcttcgaa gtctggctga
    44341 gtattgatgt tgaacggcag ccagctactg ttacctactg ccaccagcga atgatcgaca
    44401 gcattctgaa tacgaatgcc aaaaccgctg attccagtac tcagcaccgt ttcgccgttg
    44461 atggtcgtgg tggtggcctg aagctgcatt tgcaggtcgt cgctgacact gtttgagcag
    44521 ttcagggtgt agcccactgg ctgacggtag ttggcaccat caatgctttt aataatcaca
    44581 ttgccgaact ccaccacgct tccctttacc gaacagggcg gcggtccatc caccagcacg
    44641 cgcaacgtca aattgacgat tttgccgccc gccattgccg ggaaaacggt cacgccacag
    44701 gccagcaata gcgcggcaga tccggtcaga aagaacgctt tcatgttgct tctcccgtta
    44761 tcaacctttg ctgctttgat cttcatccac cttgcaggtg ttcccttcac aacggaagat
    44821 cagcggcatg cgcgcgccgt agtcgttgat ataggtcagc accggggcgc tcgccatttt
    44881 tgcctttaac ggttgtgacg ttttcggtgg catcaccagc ggtttaaacc cggcggcggt
    44941 cagccctttc ttctgtgtgc tggcatcgct gatgatgacg tggtacggcg tcgggttatt
    45001 gacggtatag tcctggccgc tgcgcgtgag tgtgacctta aactgccacg gttttctcat
    45061 gtcaatcttc tccagcgcct ttggtcgcca gaacagcttg atgcgcgttt gcagcgcaat
    45121 ttgcagggtg ttggctttcc cggtctttgg cgggatctca cgcacgttat agtagaacag
    45181 gctttcgcgg tccgacggca gcgagtgaat gtccggcagc gcctgaatct tcacctggcc
    45241 gttcatcatg gcgtcaatac gctgcaccgg cggcagtacc gccagcggcg aggtgatttt
    45301 gttgcccttc tcgtcttcca gccagctttg cgccaggtaa ggcagctttt cgttgttgtt
    45361 acgcaacgtc acgctgatcg atttatcgct ttcgttaaac accagacggg tgcgatccgg
    45421 ggtgaccgat gcatgcgccg tcaggctggc ggtggtaagc agtgccatca gcgctgtgct
    45481 gacgagcatt ggacaggata atttataaga cattgatcgt tccttaatca gattattgag
    45541 ttgctgaaat tgacgtcggc gcttccgttg gtgtgacttg ccgcgtctgg tcctgaataa
    45601 caggcttaat ttctggcgct gaagaatcag gggctgccgt tcccttttgt tcacacggca
    45661 acaacaggcc gctgaacaaa tcggcaggta gcggcttcgg caatacgatc tcgcattgtg
    45721 cgctgccttc ccagaacacc atcatatgct cgcccgcatt gacccccgcc agatagacat
    45781 tgccttcatc atccacaatg ccaacctgct gctgctcgtc gtttttcact tccgcgccaa
    45841 acggcgggta gctaccgtca cgcaaccgca gtaccgccat cgctttttgc ccgctgatca
    45901 ctttgaattt acgatagccg attgcgcctt ccgtcagagt cgcctgcacc accgactggg
    45961 tggcttccgc atcttccggc aagttgttta aatcgatata cgcctgattg cgatagtagt
    46021 tgttaatatc ggcgaccacc gccttgccaa acatattggt gtacaccggc gcgccgttgc
    46081 tttcgaccgg cacgttggca atcccgtcgg cgtcaatcag caggcgcgtg ccgcccatgt
    46141 tctgggtacg gtgcagcgcc ccaccatgcg cggtcagcgt tgccccgccc tgtaaggcga
    46201 tccccgccga acggtattcc ccttcatggt agttggcgct gagatcaacc ttcgccagcg
    46261 aaccgtcgtg gctcagataa ccatccacgc tgccgtgctg ctcgctggtg ccaacgttaa
    46321 cctggtagtg cgttgcgtca tcgacgcgct taaagtaacc gacctggctg ctgtccgacc
    46381 cgctgccgta ggaaccgttg taggtcacgg tgctgctgtc gctccaggga atgctcatac
    46441 tgaggtacat ccccttatcc gcattatcgt catattcgta gcgatagccg gtcaccgaga
    46501 tgctcatgtt gcgaatgctc cccatattaa aatagtggga aaacatcaag ttatagtttg
    46561 tctgttccgg gcgatcccag taagtacgat gggagtagtt gagatagatc gagacacccg
    46621 cagcggcaaa gttctggtta taggtgatcg tatacatctc tttgtcgtta ccggtccgcg
    46681 ccatgtccga ctggttcgcg tccagatact cgctcatggt catgaagttc ttttcagaaa
    46741 aacggtagcc tgcaaaggtg acgcggctgt tgagttcgtc aaagtctttg gcatagctca
    46801 cgcgaaagga gttgccgtcc agttttcctt tgccgtatgc gctgtcatga tccaggttga
    46861 cgtgcgagtg agtcacatcg aacgccagcg cgccaaactg tgcgaggtcg cgccccaccc
    46921 ccatcgccgc cgactgatag tgtttatctg ccagcgcacc accgtagagc gaccagccat
    46981 ctgccacccc ccatgacgct tctccgccgg agaaaaagcc gccttcggtc ttgtggttcc
    47041 agtcttccgg acgccccatc atcactttgt aacgcacctg cccctggcgc gtgaggaatg
    47101 gcatagatgc ggtcgtaacg tcatattcct gcacctgacc attctgttct tcaacgcgga
    47161 cgtgcagtgt gccggagacg gagtcgccga tatcctgaat gcggaatggc ccggctggaa
    47221 cctgggtttc gtaaagtacc cggcccatct ggctaatggt gacttttgca ctgctgtgcg
    47281 ccacgccgga aacatccggt gcatagccgc gcaggttggg cggcagcatt tggtcatcgg
    47341 tgctgacgct gctgccgata tagttaaagc cgtcgaaaat atcggaattg agataatctt
    47401 cccccagcga cagcttcgct tttaaagagg gcaaggctcg ccaggcataa taacgactcc
    47461 agtcccagtg tttactcgtt gtgctgttac tgctgtcatc gtcgtcatcg ttgctgcggg
    47521 tgtgctgata atcactttgc cagtctgcac ggaaacgcca tgcccccagg ttcgccccaa
    47581 cggtaccgtt gccgctgata tcatgtgagt cctcgccccc ctgctcctgg tggcgagttt
    47641 gcgcattgag gctgtagtcg gcaatcaggc caggaatacc gtcgtcccag cgtgagggag
    47701 ggtcccagtc gctgctggta tattcgaggt aagcctgtgg cactgtcagc agcaacgccg
    47761 actggcttaa atcattctcg acttccatcc cgtctaattg gcctggttta aggcattcat
    47821 cgttgtgcgt ccactgcagg ctttttgcta tcccttcttt cagccccagc gccgccacta
    47881 actcaggtgt caggcaggca tagttttttg ttggatcgtt ttcagagacg taccagttaa
    47941 tgtcgtattc atcagaaagg ggttgtttat ttataaatac acgtaaatta tatttacccg
    48001 cgtcgacata cccttttttt gaaaacttac cgagatcaat tttcgtatcg ccctttaact
    48061 caaggaaacg ggggtcaaat tgaattgaat catcagccca tgcattgact gacccaccag
    48121 agattgccag cgcaatatag caggcgattc cccgcagccg aaaatttgag tgattaggca
    48181 tacacatatc cgtttgtgaa acagtcgtaa atctgttgcg tgttgaataa ccccacagca
    48241 gaacgctgtg gggttgtcac atcagagata ggtaatctgg aatgtggtca gggtttcgaa
    48301 ctggccgaga tccggcgcat cggtttcacc caccagccag gctttaaagt tcagcgtttg
    48361 cttgtcttta cccttgctgg ttgaagtatc catcacgatt ggctgaacaa tcttttcgcc
    48421 cactttataa cctttgccat gggtatcgcc gattaccagg ctcacgtggt tcatggcaac
    48481 accgctgtca acattgaata acgcatagtt atctgcgttg gctggccccg cgtagttagt
    48541 gccggtgaac gtggtctcca tgtttgtttc agtggtaaac acgcaatcct gtaaacggat
    48601 ctggaaatct ttcggtgtgg tagtgccacc gccggtcagt ttagccgcac cgatgtcacc
    48661 catatcgact tccagtttgt gatcgtctgg aacgatagag cacggggagt cttcaatggt
    48721 gccgtagaag cgcgcggtac cgttgttgcc tgctgcaaaa gcagaactgg aaacaactgc
    48781 gcccatgacc atcgccgcag caatggctgt tttggcaaac ttactcatta tttgtcctta
    48841 aatcattaag agttataata taagcacctg acataaaatt aatatatgtc atgtccaata
    48901 cttatagtta gccattgaac atata
//
LOCUS       DAZFRG010000003        48146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFRG010000003 DAZFRG010000000
VERSION     DAZFRG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.003"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            252..2645
                     /gene="malP"
                     /locus_tag="ACHKUC_000111"
     CDS             252..2645
                     /gene="malP"
                     /locus_tag="ACHKUC_000111"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO6616445.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2655..4739
                     /gene="malQ"
                     /locus_tag="ACHKUC_000112"
     CDS             2655..4739
                     /gene="malQ"
                     /locus_tag="ACHKUC_000112"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO6616446.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWSEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4850..6166)
                     /gene="gntT"
                     /locus_tag="ACHKUC_000113"
     CDS             complement(4850..6166)
                     /gene="gntT"
                     /locus_tag="ACHKUC_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6616447.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6530..7105)
                     /gene="nfuA"
                     /locus_tag="ACHKUC_000114"
     CDS             complement(6530..7105)
                     /gene="nfuA"
                     /locus_tag="ACHKUC_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO6616448.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7164..7847)
                     /gene="gntX"
                     /locus_tag="ACHKUC_000115"
     CDS             complement(7164..7847)
                     /gene="gntX"
                     /locus_tag="ACHKUC_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO6616449.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7885..8655
                     /gene="bioH"
                     /locus_tag="ACHKUC_000116"
     CDS             7885..8655
                     /gene="bioH"
                     /locus_tag="ACHKUC_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO6616450.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFHHLLVALKQRV"
     gene            complement(8684..8803)
                     /locus_tag="ACHKUC_000117"
                     /pseudo
     CDS             complement(8684..8803)
                     /locus_tag="ACHKUC_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8875..9768)
                     /gene="rpnA"
                     /locus_tag="ACHKUC_000118"
     CDS             complement(8875..9768)
                     /gene="rpnA"
                     /locus_tag="ACHKUC_000118"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO6616451.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKENLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            complement(9971..10207)
                     /gene="feoC"
                     /locus_tag="ACHKUC_000119"
     CDS             complement(9971..10207)
                     /gene="feoC"
                     /locus_tag="ACHKUC_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO6616452.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            complement(10207..12528)
                     /gene="feoB"
                     /locus_tag="ACHKUC_000120"
     CDS             complement(10207..12528)
                     /gene="feoB"
                     /locus_tag="ACHKUC_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO6616453.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCISAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTSDCH"
     gene            complement(12545..12772)
                     /gene="feoA"
                     /locus_tag="ACHKUC_000121"
     CDS             complement(12545..12772)
                     /gene="feoA"
                     /locus_tag="ACHKUC_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO6616454.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(13228..15549)
                     /gene="yhgF"
                     /locus_tag="ACHKUC_000122"
     CDS             complement(13228..15549)
                     /gene="yhgF"
                     /locus_tag="ACHKUC_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO6616455.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWGDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15646..16122)
                     /gene="greB"
                     /locus_tag="ACHKUC_000123"
     CDS             complement(15646..16122)
                     /gene="greB"
                     /locus_tag="ACHKUC_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO6616456.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16350..17069
                     /gene="ompR"
                     /locus_tag="ACHKUC_000124"
     CDS             16350..17069
                     /gene="ompR"
                     /locus_tag="ACHKUC_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO6616457.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            17066..18418
                     /gene="envZ"
                     /locus_tag="ACHKUC_000125"
     CDS             17066..18418
                     /gene="envZ"
                     /locus_tag="ACHKUC_000125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO6616458.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(18495..20117)
                     /gene="pckA"
                     /locus_tag="ACHKUC_000126"
     CDS             complement(18495..20117)
                     /gene="pckA"
                     /locus_tag="ACHKUC_000126"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO6616459.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20496..22220
                     /gene="yhgE"
                     /locus_tag="ACHKUC_000127"
     CDS             20496..22220
                     /gene="yhgE"
                     /locus_tag="ACHKUC_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO6616460.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PVTVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLATALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWMPVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRQITKEKLLTAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(22283..23161)
                     /gene="hslO"
                     /locus_tag="ACHKUC_000128"
     CDS             complement(22283..23161)
                     /gene="hslO"
                     /locus_tag="ACHKUC_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO6616461.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23186..23587)
                     /gene="hslR"
                     /locus_tag="ACHKUC_000129"
     CDS             complement(23186..23587)
                     /gene="hslR"
                     /locus_tag="ACHKUC_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO6616462.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(23598..24266)
                     /gene="yrfG"
                     /locus_tag="ACHKUC_000130"
     CDS             complement(23598..24266)
                     /gene="yrfG"
                     /locus_tag="ACHKUC_000130"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO6616463.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24331..26466)
                     /gene="igaA"
                     /locus_tag="ACHKUC_000131"
     CDS             complement(24331..26466)
                     /gene="igaA"
                     /locus_tag="ACHKUC_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO6616464.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFVDAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            26786..27346
                     /gene="nudE"
                     /locus_tag="ACHKUC_000132"
     CDS             26786..27346
                     /gene="nudE"
                     /locus_tag="ACHKUC_000132"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO6616465.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(27513..30065)
                     /gene="mrcA"
                     /locus_tag="ACHKUC_000133"
     CDS             complement(27513..30065)
                     /gene="mrcA"
                     /locus_tag="ACHKUC_000133"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO6616466.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQAVRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            30185..30964
                     /gene="hofM"
                     /locus_tag="ACHKUC_000134"
     CDS             30185..30964
                     /gene="hofM"
                     /locus_tag="ACHKUC_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO6616467.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            30964..31503
                     /gene="hofN"
                     /locus_tag="ACHKUC_000135"
     CDS             30964..31503
                     /gene="hofN"
                     /locus_tag="ACHKUC_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO6616468.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAIGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            31487..31927
                     /gene="hofO"
                     /locus_tag="ACHKUC_000136"
     CDS             31487..31927
                     /gene="hofO"
                     /locus_tag="ACHKUC_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO6616469.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            31917..32321
                     /gene="hofP"
                     /locus_tag="ACHKUC_000137"
     CDS             31917..32321
                     /gene="hofP"
                     /locus_tag="ACHKUC_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO6616470.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            32233..33471
                     /gene="hofQ"
                     /locus_tag="ACHKUC_000138"
     CDS             32233..33471
                     /gene="hofQ"
                     /locus_tag="ACHKUC_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO6616471.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            33872..34393
                     /gene="aroK"
                     /locus_tag="ACHKUC_000139"
     CDS             33872..34393
                     /gene="aroK"
                     /locus_tag="ACHKUC_000139"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO6616472.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            34450..35538
                     /gene="aroB"
                     /locus_tag="ACHKUC_000140"
     CDS             34450..35538
                     /gene="aroB"
                     /locus_tag="ACHKUC_000140"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO6616473.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLNTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            35630..36916
                     /gene="damX"
                     /locus_tag="ACHKUC_000141"
     CDS             35630..36916
                     /gene="damX"
                     /locus_tag="ACHKUC_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO6616474.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEELDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQIPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            37023..37859
                     /gene="dam"
                     /locus_tag="ACHKUC_000142"
     CDS             37023..37859
                     /gene="dam"
                     /locus_tag="ACHKUC_000142"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6616475.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            37877..38554
                     /gene="rpe"
                     /locus_tag="ACHKUC_000143"
     CDS             37877..38554
                     /gene="rpe"
                     /locus_tag="ACHKUC_000143"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO6616476.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            38547..39305
                     /gene="gph"
                     /locus_tag="ACHKUC_000144"
     CDS             38547..39305
                     /gene="gph"
                     /locus_tag="ACHKUC_000144"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO6616477.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSLNDLLPALGLPHSENQESKND"
     gene            39298..40302
                     /gene="trpS"
                     /locus_tag="ACHKUC_000145"
     CDS             39298..40302
                     /gene="trpS"
                     /locus_tag="ACHKUC_000145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO6616478.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            40462..41367
                     /gene="yhfZ"
                     /locus_tag="ACHKUC_000146"
     CDS             40462..41367
                     /gene="yhfZ"
                     /locus_tag="ACHKUC_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO6616479.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            41384..41746
                     /gene="yhfY"
                     /locus_tag="ACHKUC_000147"
     CDS             41384..41746
                     /gene="yhfY"
                     /locus_tag="ACHKUC_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6616480.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            41830..42993
                     /gene="yhfX"
                     /locus_tag="ACHKUC_000148"
     CDS             41830..42993
                     /gene="yhfX"
                     /locus_tag="ACHKUC_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO6616481.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVVDAVAQGTDVITVFTLDKAREVSAAAVEAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            42994..44220
                     /gene="yhfW"
                     /locus_tag="ACHKUC_000149"
     CDS             42994..44220
                     /gene="yhfW"
                     /locus_tag="ACHKUC_000149"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6616482.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLPDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDDKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            44217..45095
                     /gene="php"
                     /locus_tag="ACHKUC_000150"
     CDS             44217..45095
                     /gene="php"
                     /locus_tag="ACHKUC_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO6616483.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSAQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            45106..45459
                     /gene="yhfU"
                     /locus_tag="ACHKUC_000151"
     CDS             45106..45459
                     /gene="yhfU"
                     /locus_tag="ACHKUC_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO6616484.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            45471..46775
                     /gene="yhfT"
                     /locus_tag="ACHKUC_000152"
     CDS             45471..46775
                     /gene="yhfT"
                     /locus_tag="ACHKUC_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO6616485.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            46787..47872
                     /gene="yhfS"
                     /locus_tag="ACHKUC_000153"
     CDS             46787..47872
                     /gene="yhfS"
                     /locus_tag="ACHKUC_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6616486.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(47980..>48146)
                     /gene="yhfL"
                     /locus_tag="ACHKUC_000154"
     CDS             complement(47980..>48146)
                     /gene="yhfL"
                     /locus_tag="ACHKUC_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO6616487.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
BASE COUNT        11329 a        12083 c        13234 g        11500 t
ORIGIN      
        1 gttaccttcc gctttttgaa aatacgcaac ggccattttt tgcacttaga tacagatttt
       61 ctgcgcggta tggcattgat ttgctgctaa tcctgtggtt tgcactagct ttaagtggtc
      121 gagatcacat ttccttgctc atccccgcaa ctcctccctg cctaatcccc cgcaggatga
      181 ggaaggtcaa catcgagcct ggcaaactag cgataacgtt gtgttgaaaa tctaagaaaa
      241 gtggaactcc tatgtcacaa cctattttta acgataagca atttcaggaa gcgctttcac
      301 gtcagtggca gcgttatggc ttaaattctg cggctgaaat gactcctcgc cagtggtggc
      361 tggcagtgag cgaagcactg gccgaaatgc tgcgtgctca gccattcgcc aagccagtgg
      421 cgaatcagcg acatgttaac tacatctcaa tggagttttt aattggtcgc ctgacgggca
      481 acaacctgtt gaatctcggc tggtatcagg atgtacagga ttcgttgaag gcttatgaca
      541 tcaacctgac tgacctgttg gaagaagaga tcgacccggc gctggggaac ggtggtctgg
      601 gacgtctggc ggcgtgcttc ctcgactcaa tggcaactgt cggtcagtct gcgacgggtt
      661 acggtctgaa ctatcaatat ggtttgttcc gccagtcttt tgtcgatggc aaacaggttg
      721 aagcgccgga tgactggcat cgcggtaatt acccgtggtt ccgccacaac gaagcactgg
      781 atgtgcaggt agggattggc ggtaaagtga cgaaagacgg acgctgggag ccggagttta
      841 ccattaccgg tcaagcgtgg gatctccccg ttgtcggcta tcgtaatggc gtggcgcagc
      901 cgctgcgtct gtggcaggcg acgcatgcgc atccgtttga tctgactaaa tttaacgatg
      961 gtgatttcct gcgtgccgaa cagcagggca tcaacgcgga aaaactgacc aaagttctct
     1021 atccaaacga taaccatact gccggtaaaa agctgcgcct gatgcagcaa tacttccagt
     1081 gtgcctgttc ggtagcggat attttgcgtc gccatcatct ggcggggcgt aaactgcacg
     1141 aactggcgga ttatgaagtt attcagctga acgataccca cccaactatc gcgattccag
     1201 aactgctgcg cgtgctgatc gatgagcacc agatgagctg ggatgacgcc tgggccatca
     1261 ccagtaaaac ttttgcttac accaaccata ccctgatgcc agaagcgctg gaacgctggg
     1321 atgtgaaact ggtgaaaggc ttactgccgc gccacatgca gattattaac gaaattaata
     1381 cacgctttaa aacgctggtg gaaaaaacct ggccgggcga tgaaaaagtg tgggccaaac
     1441 tggcggtggt gcacgacaaa caagtgcata tggcgaacct gtgtgtggtt ggcggtttcg
     1501 ctgtgaacgg tgttgcggcg ctgcactcgg atctggtggt gaaagatctg ttcccggaat
     1561 atcaccagct atggccgaac aaattccata atgtcactaa cggtattacc ccacgtcgct
     1621 ggatcaaaca gtgcaacccg gcactggcgg ctctgttgga taaatcactg aaaaaagagt
     1681 gggctaacga tctcgatcaa ctgatcaatc tggaaaaatt cgctgatgat gcgaaattcc
     1741 gtcagcaata tcgcgagatc aagcaggcga ataaagtccg tctggcagag tttgtgaaag
     1801 ttcgtaccgg tattgagatc aatccacagg cgattttcga tattcagatc aaacgtctgc
     1861 atgagtacaa acgccagcat ctgaatctgc tgcatattct ggcgttgtac aaagaaattc
     1921 gtgaaaaccc gcagtctgac cgcgtaccgc gcgtcttcct cttcggcgcg aaagcagcac
     1981 cgggctacta cctggcgaaa aatattatct ttgcgatcaa caaagtggct gacgtgatca
     2041 acaacgatcc gctggttggc gacaagttga aggtggtgtt cttgccggat tattgtgttt
     2101 cggcggcgga aaaactgatc ccggcggcgg atatctccga acaaatttcg actgcaggta
     2161 aagaagcttc cggtaccggc aatatgaaac tggcgctcaa tggtgcgctt actgtcggta
     2221 cgctggatgg tgcgaacgtt gaaatcgctg agaaagtcgg tgaagaaaat atctttattt
     2281 ttggtcatac cgtggaacaa gtgaaggcaa ttctggccaa aggctacgac ccggtgaaat
     2341 ggcggaagaa agataaggtg ctggacgcag tattgaaaga gctggaaagc ggtaaataca
     2401 gcgacggcga taagcatgcc ttcgaccaga tgctgcacag tatcggcaaa cagggcggcg
     2461 atccgtatct ggtgatggca gatttcgccg cttatgtaga ggcacaaaag caggtggatg
     2521 tgctgtaccg cgaccaggag gcctggactc gcgcggcgat cctcaatacc gcccgctgcg
     2581 gtatgtttag ctcggatcgc tctattcgcg attatcaggc tcgtatctgg caggcaaaac
     2641 gctaaggaag ctcgatggaa agcaaacgtc tggataatgc cgcgctggcg gcggggatca
     2701 gccccaatta catcaatgcc cacggtaaac cgcagtcgat tagcgccgaa accaaacggc
     2761 gtttgcttga cgcgatgcat caacgtaccg ccacgaaagt ggcggtaacg ccagtcccga
     2821 atgtcatggt ttataccagc ggcaaaaaaa tgccgatggt ggtggagggc agcggcgaat
     2881 atagctggct gctgaccacc gaagaaggaa agcagtacaa aggccatgtg acggggggca
     2941 aagcgttcaa tctaccgacg aagctgccgg aaggttatca cacgctgacg cttacccagg
     3001 acgaccagcg cgcgcattgc cgggtgattg tcgccccgaa acgctgttac gaaccgcagg
     3061 cgttgctgaa taaacaaaag ctgtggggtg cctgcgttca gctttatacg ctgcgatcgg
     3121 aaaaaaactg gggtattggg gattttggcg atctcaaagc gatgctggtg gatgtggcaa
     3181 aacgtggcgg gtcgtttatt ggtctgaacc cgattcatgc gctctatctg gcaaatccgg
     3241 aaagtgccag cccgtacagc ccgtcttctc gccgttggct gaatgtgatt tatatcgacg
     3301 ttaacgcagt tgaagatttc catcttagcg aagaggctca ggcctggtgg cagttgccga
     3361 ccacgcaaca gacgctgcaa caggcgcggg atgccgactg ggttgattac tccacggtta
     3421 ccgccctaaa aatgacagca ttacgaatgg cgtggaaagg tttcgcgcaa cgtgatgatg
     3481 agcagatgac cgcgtttcgc cagtttgttg cagagcaggg cgacagcctg ttctggcagg
     3541 cagcctttga tgcgctacat gcccagcaag tgaaagagga cgaaatgcgc tggggctggc
     3601 cagcatggtc agagatgtat cagaacgtgg attcaccaga agtgcgccag ttctgcgaag
     3661 aacatcgtga tgacgtcgat ttttatctct ggttgcagtg gctggcttac agccagtttg
     3721 ccgcctgctg ggagataagc cagggctatg aaatgccgat tggcttgtat cgtgatctgg
     3781 ccgttggtgt agcagaaggt ggggcagaaa cctggtgcga ccgtgaactg tattgcctga
     3841 aagcgtcggt tggcgcgccg ccggatatcc tcggcccgtt ggggcagaac tggggattgc
     3901 cgccaatgga cccgcatatc atcaccgcgc gtgcctatga accgtttatc gagctgttgc
     3961 gtgccaatat gcaaaactgc ggcgcattac gaattgatca cgtgatgtcg atgctgcgtt
     4021 tgtggtggat accgtatggc gagacggcag atcagggcgc gtatgttcac tatccggtgg
     4081 atgatctgct ctcgattctg gcactcgaaa gtaaacgtca tcgctgtatg gtgattggtg
     4141 aagatctcgg taccgtaccg gtagagattg tcggtaagct gcgcagcagc ggtgtgtact
     4201 cctacaaagt gctctatttc gaaaacgacc acgagaagac attccgcgca ccgaaagcgt
     4261 acccagagca gtcgatggcg gttgcggcga cacacgacct gcctacgcta cgcggctact
     4321 gggagagtgg ggatttaacg ctgggcaaaa cgctggggct gtatccggat gaagtggtac
     4381 tgcgcggtct gtatcaggat cgcgaactgg cgaagcaagg gctgctggat gctctgcata
     4441 aatatggttg cctgccaaaa cgtgccgggc ataaggcatc gttgatgtcg atgacaccga
     4501 cgctgaatcg tggcttacag cgctacatcg ccgatagcaa cagcgccctg ttagggctcc
     4561 agccggaaga ctggctggat atggcagatc cggtaaatat tcccggcacc agttaccagt
     4621 ataaaaactg gcgacgtaaa ctttccgcaa cgcttgagtc gatgtttgcc gatgatggtg
     4681 tgaacaaatt gctgaaggat ttggacagac ggcgcagagc ggcagcgaag aagaagtaga
     4741 gtgcggttga tgccggatgc gacgcttgac gcgtcttatc cggcctacta attaatgccg
     4801 aatcgtaggc cggataaggc gttcacgccg catccggcca tcagtattat caaatcacca
     4861 tattcagcag cagacagcct accagaccgc acaccgagat aatggtttcc agcatcgacc
     4921 aggatttgat ggtctcgccg atagtcaggt taaagtactc tttgaacagc cagaagcccg
     4981 gatcgttcac gtgagagaaa atcacactac cggaaccaac cgcaataacc atcagctccg
     5041 ggctaacacc cgtcgttgca atcagcggtg ccgcgatacc acccgcagtg attgccgcga
     5101 cggttgcgga acccagcgcg atacgcagta cggcagcaat cgaccaggcc atcagcagcg
     5161 gagaaatgtt ggtttcgtgc atcatggaag caatgtattt gtccacgccg ctgtctacca
     5221 gcacctgctt gaacgcaccg ccaccaccga taatcaacag catcatcgca atgattttga
     5281 tggaagaaac cagcgtgtcg ttaatctgat ccattgaacg accacggttc agaccaaagg
     5341 tgaacatcgc gatcagcacg gcaatcagcg tagccattac cgggtcaccg aggaactccg
     5401 ctaccggcag gaaagcgtga cctttcggca ggatcatttc ggcaatcgca cgcatcgcca
     5461 tcagaaccac cggcaccaga gaggtccaga cgctgacgcc aaagctcggc atctcttctt
     5521 cgctgaaggt tttcgcgctg tacagacctt ccgggatcgg cttatcgata cctttcaaca
     5581 cgcgagcgta aaccggaccg gcgagaatca cggtcgggat tgccagaata gtaccgtaca
     5641 gcagagtttt acccatatcg gcattgaaaa tggtggcaat cgcagtcgga cccggatgcg
     5701 gtggcaggaa gccgtgggtc acagacagtg cagccgccat tggtacacca acatacagca
     5761 gtggaatatt cgcggaagcc gcgatggtaa acaccagcgg cagcatcagc acgaaaccca
     5821 cttcatagaa cagggcaaaa ccaacggtaa aaccggtcag taccaccgcc cactggatgt
     5881 gttttttacc aaatttggca atcagcgtgg tagcgatacg ttgtgcacca ccgcagtctg
     5941 ccagcatttt gcccagcatc gcaccaaaac ccatgatcag ggcaaggcta ccgagcgttc
     6001 cgccgacacc ggctttgatg gagccaataa ctttatccag cggcatccct tgcattaatc
     6061 caacagcaag cgccacgagg acgagagcga tgaagccgtt cattttgaag cggatcatca
     6121 ggagtaataa caagattaca ccgatagcaa caatgactaa tggcatgatt tacctggcct
     6181 ttaatttgtt atgggtaacg tcaggtttct gacggaaaat cctggttgtc ccaactggga
     6241 acagagatgt tacggcacca cggctgatga cccctaaaac gagttagtgt agtcagatgt
     6301 tatgagtgtg tttggtgccc atgggaatga tacgggtaac attacgtgct tgagaatcac
     6361 cagagcgggg taaatttaaa ttatgagagg ttggtcatat tatcgtgggg aaacgaagag
     6421 attggatttg ctgaggtaat tctgcgctaa tgtcagactc tcgtgcaacg tacaggcccg
     6481 ataagacgct ttagcgtcac atcgggcaat cgacaaaaga ggagagaact tagtagtaag
     6541 agtgctcgcc gcgttggtgt tcggtgagat cgcgtacacc tttcagctcc gggaattcgt
     6601 tcagcaactg cttttcgatc ccttctttca gggtgacgtc gaccatggaa caaccgttac
     6661 agccaccgcc aaattgcaaa atggcgtaac cgtcttcggt gatttccatc agcgaaacgc
     6721 gaccaccgtg accagcaagc tgtgggttga tctgtgattg cagcatatac tccacgcgct
     6781 ccatcagcgg cgcatcgtca gccactttac gcattttggc gttaggcgct ttcagcgtca
     6841 gctgcgaacc caactggtcg gtaacaaaat cgatctctgc atcttccagg taaggagcgc
     6901 ttaactcatc aacatacgcg gtcagcaggt caaatttcag ggcagtgtcg gtggcttcca
     6961 cagcgtccgg cggacaataa gaaacgccac attcagcgtt aggcgtgcca gggttaatca
     7021 caaatacgcg gatttgtgtc ccttcttcct ggtttgccag cagtttggca aagtgcgctt
     7081 gtgcagcatc ggaaatacgg atcatagtaa tggcctaata gttgactatt ttagttggtt
     7141 ataatacgcc catcatcgag gctctacaag gttcgacaaa ggcaccagac ctggacagtc
     7201 gccgcaccat tgcgtaaaag caactgcgca atctctgcga cggtacttcc ggtggtaacg
     7261 acatcatcca caatcaccat atggcgacct tgcacaggca attcaaggcg aaaggcattt
     7321 ttcaggttgt gtttgcgtag ccgggcgtta agaaaatgct gggtcgcggt ggcccgtgtt
     7381 cgtgtgacgg cttcgctatc ccattggcag tgtagccagc gtgataacgg ctgacacagc
     7441 aaatcgctct gattaaatcc ccgacgccag tgacggcgct gccataacgg aacgctgatg
     7501 atgcgatcag gcagttgcaa gccggtggtg cgacgagcgt gtaagacttc caatagtaac
     7561 agacgtgaca gggcgctggc gatttcactg cgccgggaaa acttaagctg gtgaatgagc
     7621 ggacttaatg gcggcgcata gtcggcgacc gtgaccagtc tttgccaggg cagcggtttt
     7681 tgcaggcagc gaccgcaggg aagatgagag tgtgtggcgg gtaatccaca ttgtgggcat
     7741 aacgttttat ctgtacgggc ggcgcgagag cagaccgaac aaatccccca atgacctaac
     7801 gccagtggca ttcggcatag ccagcataat cccggtactg ttagcatatg ttcatccttg
     7861 taagtaaaaa gagaacaata gcggatgaat aacatctggt ggcagaccaa aggtcagggg
     7921 aatgttcatc ttgtgctgct gcacggatgg ggactgaatg ccgaagtgtg gcgttgcatt
     7981 gacgaggaac ttagctcgca ttttacgctg caccttgttg acctgcccgg cttcgggcgt
     8041 agccggggat ttggtgcgct atcacttgct gatatggccg aagtcgtgct gcgacaggca
     8101 ccagataaag ccatttggtt aggctggagt ctgggcgggc tggtggcaag ccagattgcg
     8161 ttaacccatc ccgagcgtgt tcaggcgctg gtcaccgtgg cgtcgtcgcc ttgttttagt
     8221 gctcgtgacg agtggccggg gataaaaccg gacgtgctgg cgggatttca gcagcagctg
     8281 agtgatgatt ttcagcgtac agtggagcgg ttcctggcgt tacaaaccat gggtactgaa
     8341 acggcgcgcc aggatgcgcg ggcgttgaag aaaaccgtac tggcgttacc gatgccggag
     8401 gtggacgtgc tcaatggtgg gctggaaatc ctcaaaacgg tcgatctccg tctgccgctg
     8461 caaaacgtgt ccatgccgtt tttgcgattg tatggctatc ttgatggtct ggtgccgcgc
     8521 aaagtagtgc cgatgctgga taaactctgg ccgcacagcg agtcgtacat tttcgccaaa
     8581 gcggcccatg cgccgtttat ttcgcatccg gtcgagtttc atcacctgct ggtggcgttg
     8641 aagcagaggg tgtagacagc ttttgaaatg gcgagacaga actttactga ctcgccgcag
     8701 ccaactcttc ttctgacacc ccggtaaaac gcatgatgtc cgcaagaggg actccggatt
     8761 acagcattct tttggcgata tccagggctt ccaggcgtcg acccaataaa taaccaatca
     8821 ggtaaataat aagatattct ctttctgcat ctgtcattaa aaccatcctt tacgttattg
     8881 atgatttatt tcagcaaggt cttcaaccgt caggctggta aatcgcagaa cgtcctcaag
     8941 agaaactccg gcgtccagca tggttttggc aatatttagg gcgttggtac gtataccttt
     9001 ctgaaaccct tcctgccgca atctttccgc aatagtcatt aagttctcct tgtgtttcgg
     9061 tgaatgttcg gcaacgccgt cgataaaatc gttaaaacgt accgcatcgc cggtttgcaa
     9121 gatgtaatta aacagccctt tgatttgtct gtcattagcg tatccactac ttaataagca
     9181 ggccatttgc tctaccagcc ccatcaggtc gcgttgacga atatgttttt gaattaactc
     9241 cagcagtgcc atacgtcggt gctgcatgat ttcatcatca ggcatgacgg tgacatcaat
     9301 cagcggaaat gcagaggcat aaagctgcct tgccaggttg ggatcggcga aacaatcaag
     9361 ccaacacagc gaatagggat aggggctttc aataccgtgg taaaacaaca atggcaccac
     9421 catcggcaag gttttgtatc cggcatccag gtgattttgc attgcggcaa tggcgtagcg
     9481 catcatgcga aatgcgatca gtttgtttga ggtgctttga tgttcaatca gacaatagat
     9541 gtatcctggt ccttgttccg ttttcaccga ccacagcacg tcggaatagc tttcacgcag
     9601 atcgtcatca ataaagctgc tcgactccag cttgagggtt ttcatatcac agagcgcgtg
     9661 aatgggtgcc ggtaaatgaa acgcaagaaa atcgcgtgcc gtctccggtt ggcgtaaaaa
     9721 gagtttgaac agcgcatcgt gtggggtgga actctgcttt ttgctcatgg cgttccttca
     9781 cctgttgttt gaatgccgcg actctacgcg ccactccaca cctgtgcatt agcgagtttt
     9841 catccctgcg agacgctttc agaggaattt acgtcgcgct gcaagggttg catttttatg
     9901 gaaagccgca cgcaaaaaag cccggatgca aacgcatacc gggcttttca aaacaggtga
     9961 tggagtatgg ttaacgcagc gcccaccact cgcgcaggca ggctttgcct tccgggcagc
    10021 ttttacaatt gccagagagg cagccgtcag gttcttcctg aatccgcacg gctttgccca
    10081 tgctttccag ctgttgcagc atggcgttaa tcattggctg cggggtgttc aatgtctggc
    10141 ttatctgggc cgcttccata cggccccgta acgccagcaa atcacgcacc tgaataagtg
    10201 aagccattaa tggcaatcac tggtggtgct ggctgcgcag caactgctta ccgacttgcg
    10261 cgttgccagc agttcgatat ccacccggct acgcgcgcga cgcagcaaac cgataaccac
    10321 gatgttaaac aggataaccg ccagaatgca caccaggctg taagttgggt gctggctgta
    10381 gctggcgacc tgatagaaca atgttgccag tgagtaagcg atattcagcc cccacaggat
    10441 ggagaagccc atccagccac ggcttgattc acgggcgata gcccccatca ccgagatgca
    10501 cggtacatac agcaggacga aaatcaggta gctgtatgcg gcagctgcgc taccgaattt
    10561 ctgatccatc acgcccatcg ccccggtccc catttcgccg tcgcctttgc tcgcttcaat
    10621 gggattcatc agtacgctaa ggctgaaggt gtctttcagg ctctgccagg tttcatctac
    10681 cgcgctgaac agctcttcac cgaggttaaa ttctgccgga ttgaactctt cgtcctgaat
    10741 attttctgcg gtgtagaggg tgttgagcgt gcccaccacc acttctttcg ccattgcgcc
    10801 tgtaaacagg ccaaccgttg cctgccagtt atcttcatgc acgccaatcg gcttgaagac
    10861 tggggtgatc acccggctga cggaagccag cgccgagtcg ttgatgttat cgacgatttt
    10921 tccgctcagc gagaagctgt tgaaggcgct caggaaaatg ctgacgatga taatcacttt
    10981 accagcacgc agaacgaagc ctttcagacg ctgccaggtc tggataatca ggcttttaac
    11041 gtgtggtaca tggtagaccg gcagctccat gacaaacggc gtcgcttcac cgcgcatgat
    11101 ggtgtacttg agcatcaggc cagtcagcac cgccattacg atacccagca tatacagcga
    11161 gaagaccgcc agcgcaccgt tctgcccgaa gaaggcagcc gcgaatactg cgaagatagc
    11221 cagacgcgcg ccgcaggaca taaacggtgc catcatgatg gtcatcagac gttcacgcgg
    11281 tgcatcaagc gtacgtgcgc ccattaccga cggtacgtta caaccgaaac caacgatcag
    11341 cggtacaaag gatttccccg gcaagcccag cgcctgcatc agacggtcca tcacaaacgc
    11401 cgcacgcgcc atatagccgg agtcctcaag gaaggagagg aacaggtaca tcatgccgat
    11461 ctgcggcacc agtggcagca cggtgttaat accaccgccc aggccttggg cgaggaagat
    11521 agtcagccag tccgggaagt ggagcgtgta gccaatccac tgaataccat gcacaaagag
    11581 tgctacggag ccgacgtcaa acagcggctg taacgccccg ccgatgttga tagccagcaa
    11641 gaacatcagg tacatcacaa agaggaaaat cggcagaccg aggaaacggt tgagcacgat
    11701 tttatctacc gcagtggtga aacggctggg ttctgccgtc agggtgttgc ttaccacatc
    11761 acagatggca gaaatgcact ggtaacgcgc atcggcaatg tgcagcgccg gatcgtccat
    11821 ctcattacgc agacgggcga gggcggcatc cagatgctgc gacgcttcac cggcgtaggc
    11881 gcggctgtag atatcgcctt ccagcatttg caggcccagc cagcgacgtt gtttcagcgg
    11941 gatgtcggaa ggcatcactt ttgccagtga atctgcttcg ttgagcagcg gctgagcgta
    12001 atgcaccagt tccacatttt cgttagcttt atagcgatca atcgccagct tgagcgcttc
    12061 aataccgcga ccacgggttg aaaccagcgg gatcaccggg cagccaagac gcgccgacag
    12121 agcatcaatt tcaatgcgaa tattttgctt ctcggcaatg tcgagcatgt tcagcgccac
    12181 gatgcatgga atgccgagtt ccaacagttg cagcgtcagg tacaggttac gctcaaggtt
    12241 agacgcatcc accacgttaa tcagcaggtc tgcgtcgcca ctcaaaatgt agtgacaggc
    12301 gatttgctca tcgagcgagg tctgcgatga gatggtggtc agagaatagg tgccgggcag
    12361 gtccaccagc gtgacctgat gatcggtggt ggagaattgc ccttctttac gttcgacggt
    12421 aacgccagcc cagttaccta cacgctgacg tgcgccagtg agctggttaa ataacgttgt
    12481 cttgccagaa tttggattac caattaagcc aatggttaat tttttcattg ttgttatcac
    12541 cgtattaaca ggaaaccgct tccacttcta ataaggccag atcttttttg cgtaatacca
    12601 ggctcacacg acgggtttcg atatgaatgg ggtcgccgag tggagcgacg cgcaccacat
    12661 taaaagagga gccaggtaac atgccaagag aaagcagttt ttggcgatat gccgggctga
    12721 tttcacggga aaagccagta attttccacg cagtatctgg agtgtattgc ataggtgcct
    12781 acttgtttct cattaactgg ataactacct catggggcgt ttgacagcga catgagacca
    12841 gatgatttgc caacgaagga gagattaaat aatcaacagt acaatgataa tgagaatggt
    12901 ttctatcagc aatacttaaa atgtgtgtga atgttggttt gaataataat ctgactcaat
    12961 gttgatatgg ctcaaggtta tatcgcagcg aaaatataat tcgcatgacg aattattagg
    13021 gctggttaat gtttaattaa ggtctcagtt gttaatgaag tgataaatat tagggagtat
    13081 attgtggggt tggctgtgga ataaaaatgc cggagatata ctccggcatt tttatgtcag
    13141 gcaaggcgtg tattgccaga tgcggcgtga acgccttatc tggcctacga tttcgtgaaa
    13201 atttgaaccg taggccaatc agggggttta acgcttttta cccattgctg ccgccagcgc
    13261 gtccatcatc gcgctattac cggcaggctg cgcttcacgc ccacgcggtt tggctgccgg
    13321 gcggttgttt tgcgggcgat cgttaccgcc accgcgacgg gcgttggttt cgccaggctg
    13381 ctcgtccaga cgcatggtca gggcgatacg tttacgctga agatccactt ccagcacttt
    13441 caccttcaca atgtcgcccg ctttcaccac ggtatgcgga tcttccacaa acttgttcga
    13501 caatgaagag atgtgaacca ggccgtcctg atgcacgcca atatcgacaa acgcgccaaa
    13561 gttggtgacg ttggtcaccg caccttcgag gatcataccc ggttgcaggt cgttcattgt
    13621 ctcgacgcca tcggcaaact gagcggtttt aaattccgga cgcggatcgc gacccggttt
    13681 ttccagctct ttgatgatgt cggttaccgt cggcacaccg aatttctcat cagtaaagtc
    13741 agacgctttc aggttacgca gttcgctgct gttacccatc agatctttca gtgcctgctg
    13801 tgttgctgcc agaatgcgtt ccaccaccgg ataggcttcc gggtgaacgg tagaagcgtc
    13861 cagcgggtta tcaccgtggt taatgcgcaa gaagcccgcg cactgctcga aggctttcgg
    13921 ccccagacgg ctcactttta acagttgctg acggttctgg aactggccgt tctcatcgcg
    13981 ccaggccacg atgttttgcg ccatcatgcg cgtcaggccc gccacacggg ttaatagagg
    14041 aacagaagcg gtgttgagat cgacgccaac ggcgtttacg cagtcttcta ctactgcatc
    14101 cagtttgcgt gccagctgcg tctggctgac gtcatgctga tactgaccta cgccgataga
    14161 tttcggatcg attttcacca gctccgccag cggatcctgc aaacgacggg cgatagacac
    14221 cgcgccacgc agcgaaacgt cgagatccgg gaattcctgt gctgccagct cggaagccga
    14281 gtaaaccgac gcgccagctt cgctgacgat cactttctgc gcggtcactt tcgggaactg
    14341 cttctgcacg tcgagataga aacgttcggt ttcgcgggaa gctgtaccgt tgccaatcgc
    14401 caccagttca acgttatgct tttcacacag cgcagcaacg gtcatcgctg cttttgcggc
    14461 ctgcccggta tgcggataaa tggtatcggt cgccaccagt ttgccagttg catcgactac
    14521 cgccactttt accccggtac gcagacccgg atcgaggccc atcgttgcac gcagtccggc
    14581 aggggccgcc atcagcagat cgtgcaggtt acgggcaaag acgttgattg cttcatcttc
    14641 cgcgcgttcg cgcacggtgc ccatcagttc agtttccaga tgcatcaaca ccttgatgcg
    14701 ccacgtccag ctcaccacac ctttgcgcca gctatccgcc ggggcattgt tcaggcgcag
    14761 gccaaggtga tccatgatga tttgctcgca atagctctct ttcggcggct cttcgaactg
    14821 cggatcggca ttcagcgaaa gctggagtat gccttcgtta cgaccacgga acatcgccag
    14881 cgcgcggtga gaaggcaccg tggacagcgg ttcgtgatga tcgaaatagt cgcggaattt
    14941 cgccccttcc tcttctttac cattcaccac ggttgagacc aaatgcgcgt tcttccacag
    15001 ataatcacgc actttcgcca gcagcgcggc atcttcggca aaccgttcca tcaggatata
    15061 gcgcgcgccg tccagcgcgg ctttggtatc tgccacgcct ttatcggcat caatatattg
    15121 tgcagcggcg acttctggcg tgtgtgacgg atcgccccac agcagatcgg ccaacggctc
    15181 aagccctgct tcaatggcga tttgcccacg ggtgcggcgt ttaggtttgt agggcaggta
    15241 gaggtcttcg agttcggttt tgcttagggt ggcgttgatg gcattcgcca gatcatcggt
    15301 gagtttgcct tgctcggaaa tagacttgag gatcgcctga cgtctctctt ccagctcgcg
    15361 cagatagctc agacgcgttt ccagattacg cagctgcgtg tcatccagac cgccggtgat
    15421 ttccttacga taacgtgcga taaacggcac ggtattccct tcgtcaagca ggcgaacggc
    15481 agcgtcaacc tgttccgggc gcgcctgaat ttcacccgca ataatgcggc agaacgaatc
    15541 attcatcatg gtttgagttc atcttttcgg atcaaaaatc aggggatagt tatacggact
    15601 ggctggcaaa aatgccagcc atcggcagga ggttaagact cttccttacg gtttcacgta
    15661 ctcgatagca ttaacatacc agctcgcttc cccggcaggg gtattcacca ccgccagatc
    15721 gccgacttct tttttcagca atgcgcgggc catcggggaa tcgatagaga tgtaatcttt
    15781 acggccaaaa atttcatcgt agccgacaat acggaaacgg tgagtcacac catcgtcgtt
    15841 ttcaatctcc acccacgcac caaaaaagac tttgccttcc tgctgcgggg agtaatcgac
    15901 gattttgaga ttttccatgc atttagtgag atagcgcacg cgacggtcga tttcacgcag
    15961 gcgcttttta ttatactgat agtcagcatt ttcgctgcgg tcgcccagac ttgcggccca
    16021 ggtcaccttt tttgtgacct ccgggcgttc ttcacgccag agataattaa gctcttgttt
    16081 gagtttttca tacccttccc gggtaaccag gggcgttttc atctcgttga ttccctttgt
    16141 ctgtttgata atgcgcacat tgggtataac gtgatcatat caacagaatc aataatgttt
    16201 cgccgaataa attgtatact taagctgctg tttaatatgc tttgtaacaa tttaggctga
    16261 aattcatacc agatttagct ggtgacgaac gtgagctttt ttaagaatac acgcttacaa
    16321 attgttgcga acctttggga gtacaaacaa tgcaagagaa ctacaagatt ctggtggttg
    16381 atgacgacat gcgcctgcgc gcgctgctgg aacgttatct caccgaacaa ggcttccagg
    16441 ttcgaagcgt cgctaatgca gaacagatgg atcgcctgct gactcgtgaa tctttccatc
    16501 ttatggtact ggatttgatg ttacctggtg aagatggctt gtcgatttgc cgacgtcttc
    16561 gtagccagag caacccgatg ccgatcatta tggtgacggc gaaaggggaa gaagtggacc
    16621 gtatcgttgg cctggagatt ggcgctgacg actacattcc aaaaccgttt aacccgcgtg
    16681 aattgctggc ccgcattcgt gcggtgctgc gtcgtcaggc caacgaactg ccgggcgcgc
    16741 cgtcacagga agaggcggta attgctttcg gtaagttcaa acttaacctc ggtacgcgcg
    16801 aaatgttccg cgaagacgag ccgatgccgc tcaccagtgg tgagtttgcg gtacttaagg
    16861 cgctggtcag ccatccgcgt gagccgctct cccgcgataa gctgatgaac cttgcccgtg
    16921 gtcgtgaata ttccgcaatg gaacgctcca tcgacgtgca gatttcgcgt ctgcgccgca
    16981 tggtggaaga agatccggcg catccgcgtt acattcagac cgtctggggt ctgggctacg
    17041 tctttgtacc ggacggttct aaagcatgag gcgattgcgc ttctcgccac gaagttcatt
    17101 tgcccgtacg ttattgctca tcgtcacctt gctgttcgtc agcctggtga cgacttatct
    17161 ggtggtgctg aacttcgcga ttttgccgag cctccagcag tttaataaag tcctcgcgta
    17221 cgaagtgcgt atgttgatga ccgacaaact gcaactggag gacggcacgc agttggttgt
    17281 gcctcccgct ttccgtcggg agatctaccg tgagctgggg atctctctct attccaacga
    17341 agctgccgaa gaggcaggtc tgcgttgggc acaacactat gaattcttaa gccatcagat
    17401 ggcgcagcaa ctgggcggcc cgacggaagt gcgcgttgag gtcaacaaaa gttcgcctgt
    17461 tgtctggctg aaaacctggc tgtcgcccaa tatctgggtg cgcgtgccgc tgaccgaaat
    17521 tcatcagggt gatttttctc cgctgttccg ctatacgctg gcgattatgc tattggcgat
    17581 aggcggggcg tggctgttta tccgtatcca gaaccgaccg ttggtcgatc tcgaacatgc
    17641 tgctctgcag gtcggtaaag ggattattcc gccgccgctg cgtgaatatg gcgcttcgga
    17701 ggtgcgttcc gttacccgcg cctttaacca tatggcggct ggtgttaagc aactggcgga
    17761 tgatcgcacg ctgctgatgg cgggggtaag tcatgatctg cgtacgccgc tgacgcgcat
    17821 tcgcctggcg accgagatga tgagcgagca ggatggctac ctggccgaat cgatcaataa
    17881 agatatcgaa gagtgcaacg ccatcattga gcagtttatc gactacctgc gcaccgggca
    17941 ggagatgccg atggaaatgg cggatctcaa cgcagtgctc ggtgaggtga ttgctgccga
    18001 aagtggctat gagcgggaaa ttgaaaccgc gctttacccc ggcagcattg aggtgaaaat
    18061 gcacccgctg tcgatcaaac gtgcggtggc gaatatggtg gtcaacgccg cccgttacgg
    18121 caatggctgg atcaaagtca gcagcggcac ggagccgaat cgcgcctggt tccaggtaga
    18181 agatgacggt ccgggtattg cgccggaaca acgtaagcac ctgttccagc cgtttgttcg
    18241 tggcgacagt gcgcgcacta ttagcggcac gggattaggg ctggcgattg tgcagcgtat
    18301 cgtggataac cataacggga tgctggagct tggcaccagc gaacggggcg ggctttccat
    18361 tcgcgcctgg ctgccagtgc cggtaacgcg ggcgcagggt gcgacaaaag aagggtaaat
    18421 aaaacgggag gcgaaggtgc ctcccgtttt gctttctata agatactgga tagatattct
    18481 ccagcttcaa atcattacag cttcggacca gccgctacca gcgcggcacc cgctggggtg
    18541 tcggtgtatt tatcgaagtt gtcgataaac agttttgcca gcgtttcggc tttttcctgc
    18601 cactgttccg gagaagcgta ggtgttacgc ggatcgagaa tcttcgtgtc tacgcccggc
    18661 agttcggttg ggatcgccag gttaaacatc ggcagagtga aggtttctgc gttatccagc
    18721 gaaccgttga ggatggcgtc gataatggcg cgggtatctt taatggagat acgtttgcca
    18781 gtgccgttcc agccggtgtt aaccagatac gcctgcgcgc ctgccgcctg catacgtttc
    18841 accagcactt ctgcgtactg agtcgggtgc agcgacagga atgccgcgcc gaagcaggcg
    18901 gagaaagttg gcgtcggttc ggtgatgccg cgctctgtac cagccagttt ggcggtgaag
    18961 ccagagagga agtggtactg ggtttgatcg gcagtcagac gagaaaccgg cggcagcaca
    19021 ccaaaggcgt ctgcggtcag gaagataact ttagttgcgt ggcccgcttt ggacaccggc
    19081 ttaacgatgt tttcgatgtg atagatcgga taagaaacgc gggtgttctc ggtttttgaa
    19141 ccatcatcaa agtcgatagt gccatcttca cgcacggtga cgttttccag cagcgcatca
    19201 cgacggatag cgttgtagat ttcaggttcc gcttctttcg acagcttgat agtttttgcg
    19261 tagcagccgc cttcaaagtt aaacacgccg tcatcgtccc agccgtgctc gtcatcgcca
    19321 atcaggcgac gtttcgggtc ggtggaaagg gtggttttac cggtgccgga aaggccgaag
    19381 aacaccgcaa catcgccttt ctcgccaacg ttggcggagc agtgcataga agcgatacct
    19441 ttcagcggca gcaggtagtt catcattgag aacatccctt tcttcatttc gccgccgtac
    19501 caggtgccgc caatcagctg catgcgctcg gtcaggttaa acgccacgaa gttttcggag
    19561 ttcagaccct gttctttcca ctgcgggtta gtgcacttcg cgccgttcat aacgataaag
    19621 tctggtttga aacctgccag ctcttcatcg ctcgggcgaa taaacatgtt tttgacaaaa
    19681 tgcgcctgcc aggccacttc ggtgatgaaa cggacggaaa gacgagtatc cgggttcgca
    19741 ccacagaagg cgtcgacaac gaacagacgt ttgccggaaa gttgtttggt caccaggcct
    19801 ttcagatgct gccaggtttc cggagagaga ggtttgttgt cgttcttacc tttgcctttg
    19861 tctgcccacc agaaagtatc gcgagtggta tcgtcacgaa cgatatactt atcttttggt
    19921 gaacgaccgg tgaagatccc ggtatcgacg gcaacggcac ccagattagt taacaccccg
    19981 cgctcataac cggtcaggct cgggtccagc tcttcctgat acagcagatc gtagcttggg
    20041 ttgtaaacga tatcatgtac gtcactgata ccataagcct cgagttcttg cggggtcaaa
    20101 ccattgttaa cgcgcatttc actgctcctt agccaatatg tattgcctga atagtaaagt
    20161 ctttttgggg gtgttaaccg cgacaaggct catagattta cgtatctgga gaaattcata
    20221 tttgacaaaa aactccgtga ttcctgtcac gaaacggttg ctattatcgc agaaatagca
    20281 ttttaggtgt ggaaaatgtt ctcaaaaggt taaattttgg tttatatggc gggaatattg
    20341 tgagtggaat cgcattcatg aatgaaattg atgcaaattc aatcggtaaa gtatcgattc
    20401 accacgctaa ctttttttga ttattttctg aaaaaagcaa aatactactg cccggatgcg
    20461 ataattaacc caccttttga ttctggataa ccaacatgga taacgttgaa ctttcaccgg
    20521 caacacgctg ggggatgatt gctaccggat tacttcaggg gctggtttgc tacctgctga
    20581 tcgcctggct tgccggaaaa aatctcagtt ggattgttta tggcgtgcct gtgaccgtcg
    20641 cgttttcatc cgttttactc ttctccgtga tctcctttaa acagaagcgc ctttggggat
    20701 ggctggcgct ggtgtttatt gccacgctgg gaatgagcgg ctggctgaag tggcaaactg
    20761 atggcatgac cccctggaga gctgaaaagg cgctttggga ttttggctgc tatctgctgc
    20821 tgatggcaat gttgttgcta ccgtggatac aacaaagcct tcgcatccgc aatgacagta
    20881 gccgctatcg ctacttttac cagtctgtat ggcataacgt acttatatta ctggtgattt
    20941 ttctcgccaa tggcctgacg tggctggtgc ttttactgtg gagtgagttg tttaaacttg
    21001 ttggcatcac gtttttcaaa acgctctttt ttgcaaccga ctggtttatt tatctcacgt
    21061 taggtctggc taccgcgctg gcggtgatcc tcgcgcgaac acagtcacgt ttaatcgact
    21121 ctattcaaaa gttgttcacg ttaatcgcca cggggttgct gccgttagta tcattgctaa
    21181 ccctgatgtt tattatcacc ctgccgttta cgggcctgag cgcgatttct cgccacatct
    21241 ccgccgctgg gttgctgtta acgctggcct ttttgcaatt gatcttaatg gctattgtcc
    21301 gcgatccgca aaaagcgtca cttccctgga cggggccgtt gcgttgcctg attaaaaccg
    21361 ctttgctggt tgctccgctg tatgtgttcg tcgccgcctg ggcgttatgg atgccggtcg
    21421 ctcagtacgg ctggactgtc gaccgcttgc agggcgcgct ggcggtgctg gtgttactgg
    21481 tatggtcgct ggggtatttt gtcagtatcg tctggcgtaa agggcaaaac ccccttgttc
    21541 ttcagggcaa agtgaacctt gcggtttcgt tattggtgtt ggtaatactg gtgcttctta
    21601 attctccggt gctggacagt atgcgcatta gcgtgaacag tcatatggcg cgttatcaga
    21661 gcggcaaaaa cacgccagac caggtaagtc tctatatgct cgaacagagc ggtcgctatg
    21721 gacgtgcggc gcttgagtcg ctgaaaagcg atgccgagta tatgaaagac ccgaaacgcg
    21781 cgcgggatct gctgatggcg ttagatggag agcaacatct tcaggaacag gtatcggaaa
    21841 aagtattagc cgataatgtg ttaattgccc caggttctgg taaaccggat gcgacattct
    21901 ggtcggcctt aatccaggat cgctataacg tgatgacctg tattgaaaaa gacgcctgcg
    21961 tactggtcga gcaagatctg aatagtgatg gtcaggcgga gcggatcctg tttgctttta
    22021 atgatgacag agtcattgtc tatggctttg actcagccag aaaagaatgg gacgcgcttg
    22081 atatgagttt acttccgcgc caaataacga aagaaaaatt actcacagct gcgaaggatg
    22141 gaaaactggg gacgaggcct aaagcgtggc gcgatcttgt agtggatggt gaaaggctgg
    22201 atgtgaatct gaatgagtga caaagcgcat caggtgtatt agtcgtaaaa tccggcagag
    22261 tcctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    22321 ggatctcggc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    22381 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    22441 gcgcatcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    22501 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    22561 gcagttcttc ggttttgatg gtttcggtta gcgttgccag gtggtcaaag tcgtcctgct
    22621 gggcattttg cgcaggcatt acctgcaaca acattccgcc tgcagccggt ttgccgtcta
    22681 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    22741 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    22801 cgctcggggt gatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    22861 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ctgctggttg ttgttaccgt
    22921 taataaccgc tagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    22981 acttcaacgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    23041 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    23101 gttcgccgcg aacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    23161 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    23221 cgcggcgctc ttttttgtcc gggcgtcggt ccggatgcgg catggttaag gcattaagtt
    23281 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    23341 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    23401 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg cttggcttgc
    23461 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    23521 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    23581 ctttcatggc atctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    23641 ggatggcgtt gatactgttt ctcggcaatc ccggagtcag gattagtcac gccgaggcag
    23701 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    23761 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    23821 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    23881 tgctcaagtt ttaccgccag gttgtgcgga tgagcattgg tgagcaaaat tcgctgctta
    23941 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcgcgcggt
    24001 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    24061 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    24121 tcctgtggcg taactccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    24181 tagttatcga aggcgaggtc aagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    24241 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    24301 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    24361 cagctgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtacgatg
    24421 gcgctggtaa cgacgccagg cctgtacgcc attataaatg gcgctaccca gcatcgtcag
    24481 tagcagcagc gtggtgctga gatagcgcca caggccggaa cgatccggga tcggatgaag
    24541 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cttcggcgtt
    24601 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    24661 cggatagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    24721 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    24781 ttcatgcgtg ataaacggcg cggtagaggt ggccaccagg ttatccagcg attccgcact
    24841 caccgggcgt aataacacat tcacgccatc gagtttcccg gcgttggcgc gttttaccag
    24901 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    24961 atctttggca gaacacagat ctgctgtctt cagaacaatg tcgccaaaat catcaagcaa
    25021 taccataccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag attcatcttc
    25081 cggtttaggg tgcagctggc gattaactgc ttcggtcaat gccgtcgctt tgttgaccag
    25141 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    25201 cggtaaaaaa ggtgagttgg ttttcgcgct ccaggtacct gaagtgcgaa tattacacat
    25261 tcccgtaccg ctaatacgca atgtatcgcc aacccgcacg ccagcgtcag ccagttgttt
    25321 cacgctggtg gcttcaatgg tctgcgcgcc tttcatccac gagagggtga atttcagcgg
    25381 catatccagc gggatccaga ataacagcat aaacagcacc aatagcgagc ccgcagcaat
    25441 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    25501 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcgatatcgg tttgttgacc
    25561 aagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    25621 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    25681 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    25741 cagacctgcg cccagcagca gtaacgcgcc gcctgccagc cacggaacaa agacatccgg
    25801 ggtaatcagg cagaaaaaga acatcatgaa ggaggcgaca atcagcaacg cttcgcgcag
    25861 cccgcgcgga cgactcagcg catactcttc gtgcgtttct ttgcgaatat tgagcagctc
    25921 tatttgctcg ctctcttccc cgcgaatcga cgcctgcgtt gacggaacag gttgcaaggc
    25981 atagccgcga gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    26041 cggcagcgaa tcggtatgaa tcagttcaac ggtattctca tcgttgatgt actgttccca
    26101 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    26161 ggtagagatg ccataacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    26221 attcagcgcc agagagatcg gtgccgcgct ggctcccgtt gggccaggca cctgtaatac
    26281 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcagatg tgagtttacg
    26341 cgtttgcgca tccacgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    26401 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    26461 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cggacacaat
    26521 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    26581 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg gaatatcagc aaattcctgg
    26641 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    26701 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    26761 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    26821 tgttgaaact gtagcccgtt cccgactgtt taccgtcgaa agcgtggatc tggagttcag
    26881 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    26941 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    27001 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct ttgaagccgc
    27061 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    27121 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtag cgcaagatct
    27181 ctacccggaa tcactggaag gcgatgagcc agagccgtta ccacaggtgc gctggccgct
    27241 ggcgcatatg atggatttgc tggaagcccc tgacttcaac gaagcgcgta atgtcagcgc
    27301 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    27361 cctgatgcat aatgcttatc aggcttacga agttcctgca atttattgaa ttttcaattt
    27421 tttgttggcc ggataacgcg ttcacccgca tccggtataa acaaagtgca ctttgtcagc
    27481 aaacttaaaa aggcgctgaa gcgccttttt aatcagaaca attcctgtgc ctcgccatta
    27541 tcgataatgg tcgttcccac ctcgtgcacc gcctgttgtg tcggctgcgt accttcgatg
    27601 aaatactctt cgcggctgtt gccaccatta gctaattgcc cggtgctgcg atcgatattc
    27661 accgtcacaa tacccggtgg cggcgtcagc ggctgctccg gcacaccttc aagaacggct
    27721 ttcatataag cgtcccatgc aggctgggca ctcttggcac cgccttcgta acctgagatc
    27781 tgatctttaa tcgctccgga agccgttgta tgaccgagat tacgacggtg atcatcaaag
    27841 ccaatccaga ccgaggtcac aacgcccgga ccgtaacccg agaaccacgc atctttcgaa
    27901 ctgttagtgg tacccgtttt tccgcctatg tcatgccgtt gtaaatcacg acctgctcgc
    27961 cagccagtac cctgccagcc tggttcacca aaaatattgg tgttcaaggc actcttaatc
    28021 aggaatgcca gcggtgtgtt gatgacgtgc ggtgcgtact cctgcgcgcc agtcttcgcc
    28081 actaacgcct gatttgcctg ctccagctgc ggcattggta cagaaacatt ctgctgctcg
    28141 cgggagatag cgacatcttc aacatcgtta ttttccagca cgttcgattt ctgcgtatca
    28201 ccgtaaatca ccggaatatc gcattccggg caggctactt tcggtttcgc ttcgaaaatc
    28261 acgccgccct gatcgttttc aattttgctg ataaaccacg ggtccaccag gaagccgccg
    28321 ttcgccatga ccgcgtagcc gcgcgccacc tgcattgggg tgaaggacgc tgaacccagc
    28381 gccagcgatt cggtgtggac aatgttttgt gccgggaagc cgaagcgttg cagatattct
    28441 gcagcgtagt cgacgcccat cgcccgcatt gcgcgtacca tcaccacgtt tttcgactga
    28501 cccagcccct gacgtaagcg aattggaccg gcatactgcg gtggtgagtt cttcggctgc
    28561 cagtcagaac cggcacctgc atcccagcgg gaaattggca catcgttcaa catacttgcc
    28621 agcgtcagac ctttatccat cgccgcggtg tagaggaacg gtttgatgtt ggaacccacc
    28681 tgacgcactg cctgggtggc gcggttaaac ttgctctgat tgaaatcaaa gccaccgacc
    28741 agcgccataa cggcaccgtt ttgcggattg atcgacacca gcgccgagtt cacttccggc
    28801 acttgcgcca gccaccatgc atcgccaact tgacgaaccc agatttgctg acccgtttgc
    28861 agaacatcgg tcactttacg tggcgtcggt ccctgctgag tatccgaacg gtaaggacgc
    28921 gcccagcgaa caccttccat actcaatacg acggtcgacc cgtctgccag catcgctgtt
    28981 gcttcctgag gattggcgct ggtgactgcg gcaggcagca gcggaccata ggtcggcagc
    29041 gccttcagcg tatcggtaat tttgttgttg tcccacgccg attcgcccac tttccacagc
    29101 acattcgccg ggccgcgata gccgtggcgc atgtcgtagt ccagcacgtt attacgtacc
    29161 gcctgctgtg cggcctgctg cactttgcgg gtgatggtgg tgtaaatgcg ataaccgtct
    29221 tcataggcac tttcgccata acggttatac atctcctggc gcaccatttc gctcaggtat
    29281 ggcgcagaga aagcaatctc cggcgcgtga tagttagcgt taatcgcctc agtgcgtgtc
    29341 tgatcgaact gttgttgggt gatataccct tcatccagca tccgcgacag cacgacgtta
    29401 cgccgcgcga cggcacgatc cattgagtag agcgggttga aggtggaagg cgctttcggc
    29461 agcccggcta tcaccgccat ttcgttcaac gtcagttggt cgacagtttt accgaaatag
    29521 acttgtgccg cagcaccgac accataggcg cggtaaccga ggtaaatttt gttcagataa
    29581 agctcgagga tctcgtcttt cgtcagcaac tgttcaatgc gaatcgcgag gaagacttcc
    29641 ttaatcttgc gcatcagcgt gcgttctgga ctgaggaaga agtttctcgc cagctgctgg
    29701 gtaatggtac tcgccccttg tgacgcgtga ccggagaaca gcgccacgct tgccgcacgg
    29761 aagatcccca ccgggtcaac gccgtgatgc tcgtagaagc ggctgtcttc cgtcgcgata
    29821 aaggctttca ccatctccgg cgggatttga tccaacgtaa ccggaatacg acgtttctca
    29881 ccgtattgag caatcagctc gccatcggcg ctgtaaatct gcatcggaat ttgcaggcga
    29941 acatctttta atgtcgccac atccggcagt tgtggctcga tgtagcggta taggccataa
    30001 atcgagcctg ctcccagcag aatgcaacag actgcaagga tcaaaaaata ctttacgaac
    30061 ttcactggaa atttcccatt tagtttcatt tgggcagttt ataaacaaac gcgcggtagt
    30121 ataaaggcaa gccagacgca ttgatatacc cgtcagagtg acgggtgata aggagatcat
    30181 cacaatggca tttaagaact ggcaaattgg tttgcattta caacagcaag aagcggtagc
    30241 ggttgctatc gtgcggagcg caaaagaatg cttattgcac cgctggtggc ggttgccact
    30301 ggagaacgac attatcaaag atgggcggat tgttgatgtg cagcggctgg ctaacacgtt
    30361 gttaccgtgg agtcgtgaac tgccgcagcg tcatcacatt atgttggcgt ttcccgccag
    30421 tcgcacatta cagcggtcat ttccacgccc gtcgatgtcc ctcggtgagc gggagcaaat
    30481 ggcctggctg tccgggacga tggcccgtga actggatatg gatccggact ccctgcgctt
    30541 cgattacagc gaagactcac tcagtcccgc ctataacgtg actgccgcgc aaagcaaaga
    30601 gctggcaacg ctgcttactc tggcagaaag gttgcgtgtt catgttagcg cgatcactcc
    30661 ggatgccagt gcattacagc gattcctgcc ttttttacct tctcatcagc aatgtctggc
    30721 ctggcgtgat aacgaacagt ggctgtgggc gacgcgctat agctgggggc gcaaactggc
    30781 ggtggggatg actagcgcga aggagctggc ggcagcgtta tctgttgatc ccgaaagcgt
    30841 cgcgctatgt ggcgaaggcg gatttgaccc ctgggaggcg gtttctgttc gtcagccgcc
    30901 gctaccgcca ccaggtggag actttgccat cgcgctgggg ctggcgcttg ggaaggtgta
    30961 ctgatgaacc cgccaattaa ttttttgccc tggcgacagc aacgccggac tgcttttctt
    31021 cgtttctggt tgctgatgtt cgttgcgcct ctgctgctgg ccatcgggat aacgctaata
    31081 ctgcgtctga caagcaatgc cgaagcacgc gtaaacgccg ttttgcttca ggcggaacaa
    31141 caactcgccc gcagcttaca gataacgaaa ccacgtttgc tggaacgcca gcagttacgc
    31201 gaacagcgtt tgcaacggca gcgccaacga caatttaccc gcgactggca atctgctctg
    31261 gaagcactgg cggcgctttt acccgaacat gcctggctga caacgataag ctggcagcag
    31321 ggaacgctgg agatcaaggg gctgacaaca agcattgccg cgttaaacgc gctggaaacg
    31381 tcactccgcc aggatgcttc ttttcatctc aatcagcggg gggccacgca gcaggatgcg
    31441 cagggacgct ggcaatttga gtatcagtta acaaggaagg ttagcgatga acatgttctt
    31501 tgactggtgg ttcgccacat cactccgcct ccgccagttt tgctgggcag tctggttgct
    31561 ggtgttagtt acgctcattt ttctgtcatt gacacaccat gaagagagcg acgcattaat
    31621 tcgactacgg gcaaatcatc accagcagtg ggctgcactg tatcgcctgg tagacaccac
    31681 tcccttcagc gaggaaaaaa cgctgccctt ttcgccactg gattttcagt tatccggcgc
    31741 gcaactggtt tactggcatc catccgcgca gggaggtgag ttggcgttga aaacgctgtg
    31801 ggaagcagtg ccgtcggcat ttacacggct ggcagagcgc aacgtcagcg tgagccgttt
    31861 tttgttaagc gtggaaggtg atgacctttt gttcacgcta caactggaga cgccgcatga
    31921 gggttaaacg ctggttgtta gcaggcattg cattgtgcct tttaaccggt atgcgtgacc
    31981 cttttaaacc gccggaagat ctatgccgga ttagcgaact tagccagtgg cgctatcagg
    32041 ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa agatgggcaa aaaaaatggc
    32101 gacgggtgca gcaaaacgat gtgctggaaa acggctggac gatcttacag ctgacgccag
    32161 acgcactaac gctgggaacc gggacaaact gcgaaccgcc acactggttg tggcaacggc
    32221 aaggagatac aaatgaagca atggatagcc gcactactgt tgatgcttat acccggcgta
    32281 caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt agctcaggtg
    32341 ttgcaggcgc tggctgaaca ggagaagttg aacctggtgg tttcgccaga cgtcagcggt
    32401 acggtgtcgt tacatttaac tgacgttccc tggaagcagg cactacaaac tgtagtgaaa
    32461 agcgccggat tgataacgcg ccaggagggc aacattctct cggtgcattc cgttgcctgg
    32521 cagaatgaca atatcgcccg ccaggaggcg gagcagacgc gggcgcaggc aaatctgccg
    32581 ctggaaaatc gcaatattac tctgcaatac gccgacgccg gagagctggc gaaagcgggg
    32641 gagaagctac tgagtgccaa agggagtatg accgtcgata aacgcaccaa tcgccttttg
    32701 ctgcgagata acaaaacggc gttaagcacg cttgaacagt gggtatcgca aatggatctg
    32761 ccggtcgggc aggttgagct gtcggcgcat attgtcacca ttaatgaaaa aagtttgcgt
    32821 gagttaggtg tgaaatggac gttggccgat gcgcaacaag gtggtggcgt tgggcaagtc
    32881 accacgcttg gcagcgacct ctccgtagcg acggcgacaa cgcatatcgg ttttaacatt
    32941 ggacgcatca acggacgttt actggatctt gagctttccg cgctcgaaca aaaacagcag
    33001 ctggatatta tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc cagcattaaa
    33061 caggggagcg aaattccata tcaggtttcc agcggggaaa gtggcgcgac gtcggtggaa
    33121 tttaaagagg ccgtcctggg gatggaagtc acgcccacgg tgttacaaaa aggtcgcatc
    33181 cggctgaaat tacacatcag ccagaacgtt ccggggcagg tgctacagca ggcagatggc
    33241 gaagtactgg cgattgataa gcaggagatc gaaacgcagg tcgaggtcaa aagcggagaa
    33301 acgttggcgc tgggcggcat ttttacccgt aaaaataaat cgggtcagga tagcgtaccg
    33361 ttgcttggcg acattccctg gttcgggcaa ttatttcgtc atgacggaaa agaagatgaa
    33421 cgacgcgagt tagtggtgtt tatcacgcca cgactggttt ccagtgagta aacagtcgta
    33481 aaagcggtaa tgtttttacg ctgaacgtgt ttcatctatt tgacgcgcgc aggtatttag
    33541 catacaagga gtaccgattt gagagttggt gctcttcgct gcctgcgttc catgatgatg
    33601 atttatcatt caggcggcat tttgctgtct tttttacgct aatcttaccc ggtgatttat
    33661 cgccagagcg gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca gagggatggg
    33721 tgatttattc agttgccaaa cccgctggag tattgagata attttcagtc tgactctcgc
    33781 aatatcttat gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag cgggtttatc
    33841 attaacgaat agtcttagta gtaccgaaaa aatggcagag aaacgcaata tctttctggt
    33901 tgggcctatg ggtgccggaa aaagcactat tgggcgccag ttagctcaac aactcaatat
    33961 ggaattttac gattccgatc aagagattga gaaacgaacc ggagctgatg tgggctgggt
    34021 tttcgattta gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca tcaatgagtt
    34081 gaccgagaaa cagggtattg tgctggctac tggcggcggc tctgtgaaat cccgtgaaac
    34141 gcgtaaccgt ctttccgctc gtggcgttgt cgtttatctt gaaacgacca tcgaaaagca
    34201 acttgcacgc acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa caccgccgcg
    34261 tgaagttctg gaagcgttgg ccaatgaacg caatccgctg tatgaagaga ttgccgacgt
    34321 gaccattcgt actgatgatc aaagcgctaa agtggttgca aaccagatta ttcacatgct
    34381 ggaaagcaac taattctggc tttatataca ctcgtctgcg ggtacagtaa ttaaggtgga
    34441 tgtcgcgtta tggagaggat tgtcgttact ctcggggaac gtagttaccc aattaccatc
    34501 gcatctggtt tgtttaatga accagcttca ttcttaccgc tgaaatcggg cgagcaggtc
    34561 atgttggtca ccaacgaaac cctggctcct ctgtatctcg ataaggtccg cggcgtactt
    34621 gaacaggcgg gtgttaacgt cgatagcgtt atcctccctg acggcgagca gtataaaagc
    34681 ctggctgtac tcaataccgt ctttacggca ttgttacaaa agccgcatgg tcgcgatact
    34741 actctggtgg cgcttggcgg cggcgtagtg ggtgatctga ccggcttcgc ggcggcgagt
    34801 tatcagcgcg gtgtacgttt cattcaagtc ccgacgacgt tactgtctca ggtcgactcc
    34861 tccgttggcg gcaaaactgc ggtcaaccat cccctcggta aaaacatgat tggcgcgttc
    34921 taccagcccg cttcagtggt ggtggatctc gactgtctga aaacgcttcc cccgcgtgag
    34981 ttagcgtcgg ggctggcaga agtcatcaaa tacggcatta ttcttgacgg tgcgtttttt
    35041 aactggctgg aagagaatct ggatgcgttg ttgcgtctgg acggtccggc aatggcgtac
    35101 tgtattcgcc gttgttgtga gctgaaagca gaagttgtcg ccgccgacga gcgcgaaacc
    35161 gggttacgtg ctttactgaa tctgggacac acctttggtc atgccattga agctgaaatg
    35221 gggtatggca attggttaca tggtgaagcg gtcgctgcgg gtatggtgat ggcggcgcgg
    35281 acgtcggaac gtctcgggca gtttagttct gccgaaacgc agcgtattat aaccctgctc
    35341 acacgggctg ggttaccggt caatgggccg cgcgaaatgt ccgcacaggc gtatttaccg
    35401 catatgctgc gtgacaagaa agtccttgcg ggagagatgc gcttaattct tccgttggca
    35461 attggtaaga gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa cgccattgcc
    35521 gattgtcaat cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg tctattagct
    35581 tcaggttaat tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa tggatgaatt
    35641 caaaccagaa gacgagctga aacccgatcc cagcgatcgt cgtactggtc gttctcgtca
    35701 atcttctgaa cgttctgagc gtactgaacg tggcgaaccg cagatcaatt ttgatgatat
    35761 tgaacttgat gacactgacg atcgccgtcc gactcgtgcg caaaaagcgc gtaatgagga
    35821 accggaaatc gaagaagaac ttgacgaatc cgaagatgaa accgtggatg aagagcgcgt
    35881 agagcgtcgt ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc gtcagtatat
    35941 gatgatgggt gtcggcattc tggttctact gctgttgatc atcggtatcg gttctgcgct
    36001 aaaagccccc tcgacctctt ccagcgatca aaccgcgtct ggcgagaaga gtattgatct
    36061 tgcaggcaat gcgaccgatc aggccaatgg cgtgcagcca gcgccgggaa ccacgtctgc
    36121 ggaaaatact cagcaggatg tttctctgcc gccgatctct tctacgccga ctcaagggca
    36181 aatcccggcg gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc tgaacaatgc
    36241 gctgacccag ccacaaaatc agcaacagct gaacaatgtg gcggtcaatt ctacattgcc
    36301 gaccgaaccc gcaacggttg cgcctgttcg caatggcaat gcatcgcgtg acacggcgaa
    36361 aacgcaaacc gctgaacgtc cggccactac gcgcccggct cgtcagcagg cggtgattga
    36421 accgaaaaaa ccgcaagcaa ccgtgaaaac ggaaccgaag ccagtcgcac agacgccgaa
    36481 gcgaactgaa ccagctgccc ctgtggcgag cacaaaggca ccggctgcga cttctacgcc
    36541 agcaccaaaa gagacggcga ctacggctcc agtacagacg gcatccccgg cgcaaaccac
    36601 ggcaacacca gccgctggag ggaagaccgc aggtaatgtt ggttcgttga aatcggcacc
    36661 gtccagccat tacactctgc agctgagcag ttcctctaac tacgacaacc tgaacggttg
    36721 ggcgaagaaa gagaatctga aaaactacgt tgtctatgaa acgacgcgta atggtcagcc
    36781 gtggtatgtc ctggtttctg gcgtgtatgc ttcgaaagaa gaggcgaaaa aagcggtatc
    36841 tacattgcca gcagatgtcc aggccaaaaa cccgtgggcg aaaccgctgc gtcaggtaca
    36901 ggccgatctg aagtaatcaa ggttatctcc cgcaatggtt tatcattgcg ggcgttgcct
    36961 gaagcgctgg atgctgtcgg agctttctcc acagccggag aaggtgtaat tagttagtca
    37021 gcatgaagaa aaatcgcgct tttttgaagt gggcaggggg caagtatccc ctgcttgatg
    37081 atattaaacg gcatttgccc aagggcgaat gtctggttga gccttttgta ggtgccgggt
    37141 cggtgtttct caacaccgac ttttctcgtt atatccttgc cgatatcaat agcgacctga
    37201 tcagtctcta taacattgtg aagatgcgta ctgatgagta cgtacaggcc gcacgcgagc
    37261 tgtttgttcc cgaaacaaat tgcgccgagg tttactatca gttccgcgaa gagttcaaca
    37321 aaagccagga tccgttccgt cgggcggtac tgtttttata tttgaaccgc tacggttaca
    37381 acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt gccgtttggc cgctacaaaa
    37441 aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag aatgcctttt
    37501 tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga tgatgcatct gtcgtctatt
    37561 gcgatccgcc ttatgcgccg ctgtctgcga ctgccaactt tacggcgtat cacacaaaca
    37621 gttttacgct tgaacaacaa gcgcatctgg cggagatcgc tgaaggtctg gttgatcgcc
    37681 atattccggt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg tatcagcgcg
    37741 caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc acacgtaaaa
    37801 aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg aaaaaataat
    37861 tctcaaggag aagcggatga aacagtattt gattgccccc tcaattctgt cggctgattt
    37921 tgcccgcctg ggtgaagata ccgcaaaagc cctggcagct ggcgctgatg tcgtgcattt
    37981 tgacgtcatg gataaccact atgttcccaa tctgacgatt gggccaatgg tgctgaaatc
    38041 cttgcgtaac tacggcatta ccgcccctat cgacgtacat ctgatggtga aacccgtcga
    38101 tcgcattgtg cctgatttcg ctgccgctgg tgccagcatc attacctttc atccagaagc
    38161 ctccgagcat gttgaccgca cgctgcaact gattaaagaa aatggctgta aagcgggttt
    38221 ggtatttaac ccggcgacac ctctgagtta tctggattac gtgatggata agctggatgt
    38281 gatcctgctg atgtccgtca atcctggttt cggcggtcag tctttcattc ctcaaacact
    38341 ggataaactg cgcgaagtac gtcgtcgtat cgacgagtct ggctttgaca ttcgactgga
    38401 agtggacggt ggcgtgaagg tgaacaacat tggcgaaatc gctgcggcgg gcgcggatat
    38461 gtttgttgct ggttcggcaa tcttcgacca gccagactac aaaaaagtca ttgatgaaat
    38521 gcgcagtgaa ctggcaaagg taagtcatga ataagtttga agatattcgc ggcgtcgctt
    38581 ttgatctcga cggtacgctg gttgacagtg cccccggtct tgctgctgcg gtagatatgg
    38641 cgctgtatgc gctggagttg cctgtcgcag gtgaagaacg cgttattacc tggattggta
    38701 acggcgcaga tattctgatg gagcgcgcat tgacctgggc gcgtcaggaa cgtgcgactc
    38761 tgcgtaaaac aatgggtaaa ccgcccgttg atgacgacat tccggcagaa gaacaggtac
    38821 gtattctgcg taaactgttc gatcgctact atggcgaggt tgccgaagag gggacgtttt
    38881 tgttcccgca cgttgccgat acgttgggcg cgttgcaggc taaaggcctg ccgctaggcc
    38941 tggtcaccaa caaaccgacg ccgttcgtcg cgccgctgct cgaagcctta gatatcgcca
    39001 aatacttcag cgtggttatc ggcggcgatg atgtgcaaaa caaaaaaccg catccggacc
    39061 cgctgttact ggtggctgaa cggatgggca ttgctccaca acagatgctg tttgttggcg
    39121 actcacgcaa tgatattcag gcagcaaaag cggcaggttg cccatcagtt ggcttaacct
    39181 acggatataa ctacggcgag gctatcgatc tcagccagcc tgatgtaatt tatcagtctc
    39241 taaatgacct tctgcccgca ttagggcttc cgcatagcga aaatcaggaa tcgaaaaatg
    39301 actaagccca tcgtttttag tggcgcacag ccctcaggtg aattgaccat tggtaactac
    39361 atgggtgcgc tgcgtcagtg ggtaaacatg caggatgact accattgcat ttactgtatc
    39421 gttgaccaac acgcgatcac cgtgcgccag gatgcacaga agctgcgtaa agcgacgctg
    39481 gatacgctgg cgttgtatct ggcttgtggt atcgatcctg agaaaagcac catttttgtt
    39541 cagtcccacg tgccggaaca tgcgcagtta ggctgggcat tgaactgcta tacctacttc
    39601 ggcgaactga gccgcatgac acagtttaaa gataaatctg cgcgttatgc cgagaacatc
    39661 aacgctggtc tgtttgacta tccggtgctg atggcagcgg acatcctgct gtatcaaact
    39721 aatctggtgc cggtgggtga agaccagaaa cagcacctgg aactgagccg cgatatcgcc
    39781 cagcgtttca acgcgctgta cggcgatatc tttaaggtgc cagagccgtt tattccgaaa
    39841 tctggcgcgc gcgtaatgtc gctgctggag ccgaccaaga agatgtccaa gtctgacgat
    39901 aaccgcaata acgttatcgg cctgctggaa gatccgaaat cggtagtgaa gaaaatcaaa
    39961 cgcgcggtca ctgactccga cgagccgccg gtagttcgct acgatgtgca gaacaaagcg
    40021 ggcgtttcca acctgctgga tatcctttct gcggtaacgg gccagagcat cccggaactg
    40081 gaaaaacagt tcgaaggcaa gatgtatggt catctgaaag gtgaagtggc tgatgccgtt
    40141 tccggtatgc tgactgaatt gcaggaacgt taccaccgtt tccgcaacga tgaagccttc
    40201 ctgcaacagg tgatgaaaga cggcgcagaa aaagccagcg cacacgcttc ccgtacgcta
    40261 aaagcggtgt acgaagcgat tggttttgtg gcgaagccgt aatttaacat taatgccaaa
    40321 aaccggggaa acccggtttt tttatcctca tttgcaataa tccgaaaaaa tgtgaagcgc
    40381 ctcgccgttt ccacatctcg ctgttgcgcc ttatcttcaa attcctgaag atataaacat
    40441 tcattttatt gaaaatatat tatgcgtcga acgtttatca aaaaagaagg cgtcgttatc
    40501 acgacactgg cccgttattt gttgggtgaa aagtgcggta atcgactgaa aaccatagat
    40561 gagctggcaa ctgaatgccg ttcatcggtt ggcctgacgc aggccgcgtt gaaaacgctg
    40621 gaatcaagcg gagcgatacg gattgaacgc cgtgggcgca atggcagtta tctggtcgag
    40681 atggataaca aagcattgct gactcatgtg gatatcaaca acgtggtatg tgcgatgccc
    40741 ttgccctata cccgtttgta cgaaggcctg gcgagcggat tgaaagccca gtttgatggc
    40801 attccttttt actatgcgca tatgcgcggc gcggatattc gcgtggagtg tttacttaac
    40861 ggcgtgtatg acatggcggt ggtttcgcga ctggcggcgg aaagttatct cacgcaaaaa
    40921 ggattatgcc tcgcgctgga gttgggaccg catacctacg ttggcgagca ccagttgatt
    40981 tgccgtaaag gcgagtccgc aaacgtgaag cgcgtggggc tggataaccg ttcggcagat
    41041 cagaaaatca tgaccgatgt cttttttggc gatagtgatg tggaacgagt cgatctctct
    41101 tatcacgaga gtttacaacg cattgttaaa ggcgatgttg acgcggttat ctggaacgta
    41161 gtggcggaaa acgaactgac tatgctggga ttagaagcga cgccgctcac agacgatccg
    41221 cgatttttac aggccaccga agccgtgatc ctgacgcgtg ccgatgatta cccaatgcaa
    41281 caactgctgc gtgcagttgt tgataaacat gccctgcttg ctcatcaaca gcgagtagtc
    41341 agtggggaac aggaacccag ttattaaacg aaaggcatct gatatggaaa ccagactcaa
    41401 cctgctttgc gatgcaggcg ttattgataa ggacatctgc aaaggcatga tgcaggtcgt
    41461 caatgtactg gaaacagagt gccatctacc ggtgcgcagt gagcaaggaa cgatggcgat
    41521 gacacatatg gcgagtgcac tgatgcgcag tcgccgtggt gaagaaatag agccgctgga
    41581 tgacgaattg ctggcggaac tggcgcaatc cagccactgg caagccgttg tgcaattgca
    41641 tcaggtattg ttgaaggaat ttgcactgga agttaacccg tgtgaagaag gctatttgct
    41701 ggccaacctt tatggattat ggatggctgc taacgaagag gtttgaagct cccgataaat
    41761 ccacgtcgta caacctaaat attcaaaaaa atgaatattt aattcaagga acagaaacga
    41821 ggcaacaaga tgtttgtaga agcattgaaa cgccagaatc cggcgctgat ttccgccgca
    41881 ctaagtttgt ggcagcaggg caagatcgcc ccggacagtt gggtgatcga cgtggatcaa
    41941 gttctggaaa acggcaagcg gctgattgag acggcgcggc tttacggcat tgaactgtat
    42001 ctgatgacca aacaattcgg tcgtaatccg tggctggcag aaaaactgct ggctctgggc
    42061 tacagcggca ttgtggcggt ggattacaaa gaggcgcgag tcatgcgccg cgccggtttg
    42121 ccggtagcgc atcaggggca tttggtacaa atcccttgtc atcaggtggt tgacgccgtt
    42181 gcacagggaa ccgacgtcat caccgtgttt actctcgaca aagcgcggga agtttctgcg
    42241 gcagcggtgg aggccggacg tgtgcagtct gtgctgctca aagtttatag cgacgatgat
    42301 tttctttatc cggggcagga gagcggtttt gtacagcatt cgctgcacga ggtggttgct
    42361 gaaatccaga atctgccggg gctacatttg gcgggactta cccatttccc ttgcctgctt
    42421 tgggatgagg ctgccgggaa agttttgccg acaccgaatc ttcacacgct ggtacaagca
    42481 cgggatcaac tggcgaaatc tggtattgcg attgagcaac tgaacgcacc ttcagcgacc
    42541 agctgcacgt cgctgccatt gctggcggaa tacggtgtga ctcataccga acccggtcat
    42601 gcgctgacgg gcactattcc ggcaaatcaa cagggcgatc agcctgaacg tatcgcgatg
    42661 ctctggttaa gtgaaatctc ccatcatttc cgtggcgaca gctactgcta cggcggcggt
    42721 tactaccgtc gtggtcacgc gcaacatgcg ctggtgttta cgccagaaaa tcaaagaatt
    42781 actgaaacat atctcaacgc cgtggatgac agcagtatcg actacaccct gccgctggca
    42841 ggcgagcatc cggtaagtag tgcagtggtg ctctgttttc gcacgcagat ttttatcacc
    42901 cgtagcgatg tggtgctggt gtccggtatt caccatggcg aaccagaaat agttggtcgt
    42961 tatgacagcc tcggcaaccc tctggaggcg taaatggcgc gatttgtggt gttagtgatt
    43021 gatagctttg gcgtaggggc aatgaaagat gtcacgctgg tgcgtccgca ggatgccggg
    43081 gcgaatacct gtggtcacat cctgagccag ttgccgcatt tgcagctacc agcgctggag
    43141 aagctggggc taatcaacgc attgggttat gcaccaggtg atatgcaacc gtcagattcc
    43201 gcaacctggg gggtggcaga gctgcaacat gaaggtggcg atacctttat ggggcatcag
    43261 gaaattttag gcacgcgccc gttaccgccg ctgcggatgc cctttcgcga tgtgattgac
    43321 cgtgttgagc aggcattagt ttccgctggc tggcaggtgg agcgccgtgg cgatgatctg
    43381 caatttctgt gggtcaatca ggcggttgcg attggcgata atctcgaggc ggatttaggc
    43441 caggtctata acatcaccgc caatctctct gtgatctctt ttgacgacgc aatcaaaatt
    43501 ggtcgtattg tgcgtgagca ggtacaggtc ggtcgggtca ttacatttgg cggcctgtta
    43561 cccgacagtc aacgcattct cgatgccgca gaaagcaaag aagggcgctt tattggtatc
    43621 aatgcgccgc gttcaggcgc ttatgacaac ggcttccagg tcgtgcatat gggctacggt
    43681 gtcgatgata aagtgcaggt gccacaaaaa ctgtatgaag tgggcgtacc aaccgtgctg
    43741 gtggggaaag tggcggatat tgccagtaat ccttatggcg tgagctggca aaacctggtg
    43801 gatagccagc ggattatgga tatcaccctc gacgaattta acacgcatcc gacggcgttt
    43861 atttgcacca atatccagga aaccgacctc gctggtcacg cggaagacgt cgcacgttat
    43921 gccgaacgtt tgcaggtcgt tgaccgtaac cttgcccggc ttgttgaggc gatgcagcca
    43981 gatgattgcc tggtcgtgat ggcggatcac ggtaacgatc cgaccattgg tcacagccac
    44041 catacccgtg aagtggtgcc agtgctggtt tatcagcaag ggctggtcca cacgcaactc
    44101 ggtgtgcgca ccacgctttc tgatgtgggg gctaccgtgt gtgaattttt ccgtgcgcca
    44161 ccgccacaaa atggtcgctc ttttctttcc tccctccggt ttgcaggaga caccctatga
    44221 gttttgatcc gacgggctac accctcgccc atgagcatct gcatattgat ctctccggct
    44281 ttaaaaacaa cgtggactgc cgccttgatc agtatgcgtt catttgccag gagatgaacg
    44341 acctgatggc ccagggcgtg cgtaatgtga ttgagatgac caaccgttac atggggcgca
    44401 atgcgcaatt tatgctcgat gtaatgcgcg aaaccgggat caacgtggtg gcctgtaccg
    44461 gttattacca ggacgcgttt ttcccggaac atgtggcgac ccgcagcgcg caggaactgg
    44521 cgcaagagat ggtcgatgaa attgagcagg gtatcgatgg cactgacctg aaagccggga
    44581 tcatcgcgga gattggctcc agcgaaggga agattacgcc gctggaagag aaggtgttta
    44641 ttgccgccgc gctggcacat aaccagaccg gacgcccgat ctccacgcat acgtcgttca
    44701 gcacgatggg gctggagcaa ctggctctgt tgcaagccca cggagttgat ctttcgcgcg
    44761 tcaccgtcgg ccactgcgat ctgaaagaca atctcgacaa cattttgaag atgatcgatc
    44821 tcggcgcgta tgtgcagttc gacaccatcg gcaagaacag ttactacccg gacgaaaagc
    44881 gcattgcgat gctccatgcg ctacgtgacc gtgggttgct gaaccgcgtc atgctgtcga
    44941 tggatattac gcggcgctct catttaaaag ccaacggtgg ttatggctat gactttttat
    45001 tgaccacctt tattccgcaa ttgcgccagt caggattcag tcaggccgat gtggatgtga
    45061 tgttacgtga aaatccctct caatttttcc aataaggaca gactcatgaa aaagattggc
    45121 gttgcaggct tacagcgtga gcagattaaa aaaaccattg aagcgacggc tcctggctgt
    45181 tttgaagttt tcattcacaa cgacatggaa gcggcaatga aagtgaaatc cggacaactg
    45241 gattactaca tcggcgcgtg taataccggt gcgggcgcgg cattgtcgat tgccatcgcg
    45301 gtgattggct ataacaaaag ttgcaccatt gccaagccgg gcattaaagc gaaagacgaa
    45361 catatcgcca aaatgatcgc tgaaggaaaa gtggcgtttg gcctttccgt tgagcacgtc
    45421 gaacacgcga ttccgatgct gattaaccat ctgaaataaa aggcacgact atggatctgt
    45481 acattcagat tatcgtggtg gcgtgcctga cgggtatgac atcgcttctg gcgcatcgct
    45541 cggcagccgt ttttcatgac ggtatccgcc cgatcctgcc gcaactgatt gaaggctata
    45601 tgaaccgtcg cgaggcgggg agtatcgctt ttggtctgag cattggtttt gtggcctcgg
    45661 tggggatctc ttttaccctg aaaaccgggc tgctcaacgc atggttactc tttcttccta
    45721 ccgatatcct cggcgtactg gcgataaaca gcctgatggc gtttggtctt ggtgctatct
    45781 ggggaatatt gatcctcacc tgtctgttgc cagtaaacca gctgctgacc gcgctaccgg
    45841 tggatgtatt aggtagcctg ggggaattaa gctcgccggt ggtttcagct tttgcactgt
    45901 tcccgttggt ggcgattttc taccagtttg gctggaagca aagtctgatc gccgccgtgg
    45961 tggtactgat gactcgtgtg gtggtagtgc gctatttccc acatcttaac cctgaatcca
    46021 tcgaaatctt tattggcatg gtgatgctgc tggggatcgc aataactcat gacctgcgcc
    46081 atcgtgatga aaatgatatc gatgccagcg gtatgtcggt gtttgaagag cgtacatcac
    46141 ggattatcaa aaacttaccg tatatcgcca tcgtgggtgc attgattgct gccgttgcca
    46201 gcatgaagat tttcgccggt agtgaagtgt cgatcttcac actggagaaa gcgtactccg
    46261 caggcgtaac gccggaacaa tcgcaaacac taatcaacca ggcggcactg gcagaattta
    46321 tgcgcgggct gggttttgtg ccgttgattg ccaccaccgc gttagccacc ggtgtgtatg
    46381 cagttgcggg ctttaccttt gtttatgcgg tgggctatct ctcgccgaat ccgatggttg
    46441 cagcggtatt aggcgcagtg gttatttcgg cggaagtctt gctgcttcgt tcgatcggca
    46501 aatggttggg gcgctacccg tcggtacgta atgcgtcgga taacatccgt aacgccatga
    46561 atatgctgat ggaagtggcg ttgctggtcg gttcgatctt tgcagcaatc aaaatggcgg
    46621 gctataccgg attctctatc gcggttgcca tttacttcct caacgaatcc ctgggccgtc
    46681 cggtacagaa aatggcggca ccggtcgtag cagtaatgat caccggcatc ctgctgaatg
    46741 ttctttactg gcttggcctg ttcgttccgg cttaaggaag gcacctatga agacgtttcc
    46801 tctgcaaagc ctgacgctta ttgaggcgca gcaaaagcag tttgcgctgg tggacacgat
    46861 ttgccgtcat tttcctggca gcgagtttct tacctgcggt gatttgggct taacgccggg
    46921 gctaaatcag ccccgcatta cccagcgtgt ggaacaggtg ctggctgacg cgtttcacgc
    46981 acaggccgcg gcgctggtac agggcgcggg tactggcgcg atccgtgcgg cactggcggc
    47041 tttgctcaaa ccggggcaac gtcttctggt gcatgacgcg ccggtttacc caacgacacg
    47101 ggttattatt gagcagatgg gactaacgct cattacggct gatttcaatg atctgtcggc
    47161 actgaagcag gttgttgacg agcaacaacc tgatgcagcg ctggtgcagc atacgcgcca
    47221 gcagccgcag gaccgctatg tgctggcaga cgtgctggca acgttgcgcg cggcaggtgt
    47281 tccagcgtta accgatgaca actatgcggt gatgaaggta gcgcgaatcg gctgcgaatg
    47341 cggggcgaat gtgtcgacat tttcctgctt caagctattt gggccagagg gcgttggtgc
    47401 ggtggtaggc gatgccgatg ttattagccg gattcgcgcc acgctctatt ccggcggcag
    47461 tcagattcag ggggcgcagg cattagaggt gttgcgtggt ctggtgctcg cgccagtgat
    47521 gcacgcggtg caggcggggg tatctgaacg gttgctggct ttgcttaacg gcggcgcggt
    47581 ggcggaagtg aaaagcgccg tcattgccaa tgcacagtcg aaggtattga ttgtggagtt
    47641 tcatcagccg attgccgcca gagtgctgga agaggcgcag aaactcggtg ccttgcctta
    47701 cccggttggt gcagagtcga aatatgaaat cccgccgctc ttttatcgac tttccggaac
    47761 gtttcgccag gcgaatccgc agttagaaca ttgcgcgatt cgcattaacc caaatcgcgg
    47821 cggtgaagag acgatattgc ggatcttgcg tgaaagtatt gccagcattt aatcgttgta
    47881 aacgatattt ttgaaaagaa tgcagaaaca aatacgccct cggataactc cgagggcgtc
    47941 aggcaattaa accggcgtta gccgatttct gtcaatagct tactgtgcag tcggaccgca
    48001 accgccaatg attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt
    48061 cttatcacgg ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcgcctac
    48121 cagtgcaact ttaataaatt tgttca
//
LOCUS       DAZFRG010000004        45442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFRG010000004 DAZFRG010000000
VERSION     DAZFRG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.004"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..9646
                     /gene="clbB"
                     /locus_tag="ACHKUC_000155"
     CDS             26..9646
                     /gene="clbB"
                     /locus_tag="ACHKUC_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin hybrid non-ribosomal peptide
                     synthetase/type I polyketide synthase ClbB"
                     /protein_id="HFO6616488.1"
                     /translation="MDNTSGDFPCNKMDTRKQLPLTPSQQGFLFHSLKDKKRSNYHEH
                     FTCIFSQHVDSAHFKWALETLFRKHECFRTDYNWEIDERPCQVVKTDVLPDIYVLDCE
                     QEEIRFLLANDDIIIPVPQDDGIDAIIPQLLQADLKYPFSLKTIPVRAYLIQSTKESA
                     FILSYHHIVMDGWSLSLFIKQLLQLYGAAVVSGVRDDSAIIPSSLKPLVDTLSARRHT
                     FQHDYWAAYLREGTPTCIVPLSQYHTDTEAENNSYVNQTNHVEINLSPDVCQKIQTLC
                     SDYRITPAVIFYVAWGILLQRWCYADDVLFGATISGRNIPIDGIEETLGLFINTLPLR
                     LRDDGATLLQHLQRMHQTLIAHYSNEHDALASIQRLVHKEGHAGDLFNTLVVLENYPV
                     DMTLLSCASPVAIRHLSVHEQTHYPLTLTITQQKGFRFSIAYALNYLTNNMAQALLMH
                     LSYLLEQLVDNPQRPIAALVNLSPCQQAQVLQPYLERMACRDWDSQSNVIEQFHQVAA
                     TSPAQVAVVDELCALTYSELAAQAEQLAAYLVQQGVMVGDTVGIISERRVNTVVAIIA
                     IMLIGAAYVPISPDYPVGRMQEIIDDSGLALLLVHGKPLDALNVAQSDLCAFPVAPSV
                     VFPVITPDSRAYVIYSSGSTGKPKGIAVAHRGLLRLIQGDSPLKVESGETTLLTCPFE
                     FDVSVFEMWSTLLNHGKLVLLSKQALLDINHIRRTIADEQVARAWFTSSLFNSYVAEG
                     ADFFGMLQHITVGGEAVSAWHVNDVMQKYPHLVVTNGYGPTENTIFTTAYRFNGLQPA
                     RVPIGYAVPGTSLYITDLHGHLLPIGATGELVAGGVGVAIGYQNNPALSATVFVPDPF
                     IPGGMMYKTGDYARLLDDGCVDCFGRKDGQIKINGQRIETGEIEQRLLECSGIIEAVV
                     VPYRVRETLHIAAVVCVNDSYDEVEVRGQLADRLPPFAIPESLVVVTEIAKSHSGKAD
                     LAQLRYLLPATQCNAVSTTISEVHSDMEHALHAIWQRVLDRQDIDSNASFFALGGTSL
                     DTIRVKGDIKRQLGLEIDITDLFKYPTLTALAHFLDTAVSPEDAIPTRAVVYSDMPVA
                     IVGMAGRFPGAANIAALWTLVVGGESGLTLFSDEELRAHGVTPDTLKQANYIKTKGIV
                     DDHEWFDADFFGYTPNEAECMDPQIRLLHQCCWQTLEHAGCDPATFTGAIGIYAGLLT
                     SPHWLNAVMQDTTDSTALYKASILNIHSVTALIAHALNLTGPAVTLDTACSTSAVAIH
                     QACIALRNRDCDAALAGGVSIEMPAYRGYEYHEGMINARDGVCRPFDSQASGTVTGDG
                     LGMLLLKRLDDALADRDCIYGVIKGSAVNNDGNNKIGYTAPSVIGQSTVIRTSLRRAG
                     FDSDSIGLVEAHGTGTVLGDPIELRALNEVFGPTPVPFCVVSALKSNIGHLNSAAGVA
                     GVIKTTLALHHQVLPPTAHFRQLNPAIDLSRSALYVNQQVQPWPSTRPRRALVSSFGI
                     GGTNASIALEAHQHEDDPSATGVRDSYLLLFSAKTPAALELRVASTLEYVKHGVGVRL
                     PDVAYTLQTGRTAFDHRRAYLVSRGSKIDLSCATILQAEIFNGQRTTAEICFMFPGQG
                     SQYHGMASALYAHQPMFRQHMDRCFAAFQRYSTVDLKALLFDDEDTRDIDQTQFTQPA
                     LFCVEYSLARTLIDLGITPDSMIGHSLGEYVAACIAGVFTLEDALHVIEARGRLMQSM
                     RPGSMMAVYLSREQLTPWLAAERGIELAANNSAHFCVVAGEQAAISRLSTRLVEGGIQ
                     HRRLKTSHAFHSAMMTPMLHDFAQLLGQIPMHAPHKRFISNVSGTWITEEQATSPDYW
                     VQQVRNAVLFSEGAAQLLVQPTLFIECGPGNTLSTFIQGHNQYSDQPTLLTLRKANAA
                     IDDEHMLHRTLAALWVRGENIDWRRFNQTALGKHIPLPDYPFEQTYYYRYGAALSGYR
                     QYPNPLRRPQDEWLQRVLWRMHDTSLREAFYAPGELIIIISADGDKLQQTLMSSGVDS
                     ITMPLPISSEDDVWDNDRILTHFHDICALLAHKTYRQLHCLYAPGAEAGSSLTQSLSG
                     LYRVARWCMHSTTPLASLTVLTHGAFRVQEEDNPEPTLAALSGAVNVFAQELHPTEVR
                     LIDIDAQSSDENLNLLTQRLAPKQETVMALRQGMLYLRRFIPTRLLAHLPPQTGCIPG
                     NVLWIIGGEKGIGRMIGEALAQREGVRVVLSSRTGYHHEAVQQDALDVIHCDVTQAEA
                     VRACLATLLERYGRLDGVIFAADATTTLTLHQLSESALRDTLTVKERGTANVLHALAQ
                     RNLLDERLLLLFCNSLAAVNAEIGQTGYATASAYLDALAQQLRTRYKVNALSIGLDAL
                     REQGMLLDAINGSEYDVLRGLRPLMTGTLLQAYKQQGADTSYYARLSPESDWLLDEHR
                     ISGIATLPGTGYLALAYEALRHYFVQDQICIDELVFLAPLTVMDNCSVDVFVDISPNG
                     QGVSVEVKSMTERFSGTLTTHARGRATRLMVDDNVVCDLTGLMREMHTITPPTKELSS
                     THFHYGPRWHSVQQLYGNTAQTQVFATLALPTVAANDTIALHPALLDIASSVVEQLPG
                     FHTDSVPFLYQDLRLYRPLPNTLHVALTVNRHDEEGDSYAFTLYDMAGEMVARCAAMV
                     KRKVQLHIQDVDDDTRLRVPSADNYQLRLAAEGEGAGKLALCPTPRLALGDSQVEIEV
                     LATGLNFKDVLFTTGLLRQQPGEAPLQLGLECAGRITRVGKNVTEFAPGEDVMAVLNG
                     GFVQYARVESDCVVRKPAHCRIEQAAALPIAYLTAYYALVVRANLQPGERVLIHSAAG
                     GVGLAALHIAKRCGAQIFATAGSEQKRDYLLSLGVHAVADSHDEQFAATLLTASDGQG
                     MDVILNSLTGRLLDASLALLAPLGRFLELGSKDIVEDKALPMRFFAQGGTFIPINFHA
                     AHGAFSRYLQQIVAWIDDNTLPLLPCKSVPLPEVARAFATLTTPQHIGKVVVTHRTAA
                     GMDRLNAMIAERRLGGYALSMSNAEVMRQLWPILNTRSPWAQLLLSPRAIDRLARGNR
                     VDRGVPSAANDTITQQTVKKRPRPEIGVPYSPATREVERVLCQILEEYLGLDRVGIDD
                     NYAELGATSLDMVQLSGQMARHYPQVSVVSLYNHATVRQLATFCQPPEGESNAPSPQP
                     AVQTNTRANQIAKRALQIAKNTARSHTSLH"
     gene            9687..12287
                     /gene="clbC"
                     /locus_tag="ACHKUC_000156"
     CDS             9687..12287
                     /gene="clbC"
                     /locus_tag="ACHKUC_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbC"
                     /protein_id="HFO6616489.1"
                     /translation="MEYASEMNGMEIAIIGMAVRFPQSRTLHEFWHNIVQGKECVTFF
                     SEEELLAEGVEQSTLDNPAYVRAKPYIEGICDFDAAFFGYSHKEAQTLDPKSRVLHEV
                     AYHALEDAGYAQRTSDLITGVFVGASEDVDWLRRSLSQIGGDALNRFESGIYGHKDLL
                     AHLIAYSLNLNGPVYSLYTSCSTSLSATHIACRSLLFGECDLALAGGITIDLPQKSGY
                     FCQQGMIHSTDGHCRPFDSQASGTLFGDGAGVVVLRRLEDALAAGDRIYAVIRGSAVN
                     NDGKQKIGFVAPGHEGQKAVICAACHLAEVSPESIGYVETHGTGTRIGDPIEFAALTE
                     AFDTSHRQYCALGAVKANIGHTHAAAGVAGLIKTALVLHHRTIPPLANYQMPNSKLDL
                     AHSPFYIPIQPQEWPASRMPPRAGVSSFGIGGTNVHMILEGLNPAVRDDHDQVRAPVF
                     IPLSAPSFEQLDELTQQLTPLLATLDASTLAYTQQVARPVFDCRRVIQVENDGTQAML
                     ASLDNLMPDAPWGLHCPDLRTTNDCTYAQWLAHSAHYQREATALTALLDGMNIPPAYC
                     HAETWAAQANSSLLIRGCQTIAALKTWMNLLPTLTLLSGAGTGLLPAAAASGMIATQD
                     VLHLLWEMEQKALHLWLPERHEPIPGYVLAWQGNPITDAQRNDRGFWSEALLADTREL
                     GEGVHSINWVRLPPEIREDVDVLRYVAQLWCAGINVDWAVWYGTPLPQRGSASAYPFA
                     HNHYPLPGRVMGSVETQPEAGPETHHPYQARPVLSVPFVAAHSRGMQYITGLMELLLE
                     ISPVGVDDDFFELGGHSLLVTQLTSRLERDFNVHIDLLTLMENPNPRNIYAHIAAQLG
                     GEDNLEIACQ"
     gene            12297..13166
                     /gene="clbD"
                     /locus_tag="ACHKUC_000157"
     CDS             12297..13166
                     /gene="clbD"
                     /locus_tag="ACHKUC_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis dehydrogenase ClbD"
                     /protein_id="HFO6616490.1"
                     /translation="MMNVAVIGAGVMGTGVAHNMAQYGISTNVVDISQSQLDKCRQMI
                     EANLRLYNFHPQHKKKTHSTAEIMENIRFTTELDDIVECDLVIENITEDIEKKNALYT
                     RMNTICGASTVFGVNTSAISITALSKLMRHPENVVGVHFMNPVPLMHTVELIRGVHTA
                     ERTLNIFHHLFAQLNKTGIVVNDSPGFVTNRAMMIFVNEAIFMVQEQIARAEDIDTLF
                     KTCFGHKMGPLQTADLIGLDTILQSLQVLYESFNDDKYRPSFLLKKMVDAGYLGVKSG
                     QGFYRYQQTYAEQ"
     gene            13196..13444
                     /gene="clbE"
                     /locus_tag="ACHKUC_000158"
     CDS             13196..13444
                     /gene="clbE"
                     /locus_tag="ACHKUC_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis aminomalonyl-acyl
                     carrier protein ClbE"
                     /protein_id="HFO6616491.1"
                     /translation="MKKQDMKAAIREFLSRSLRGHTLNDDDDIFSLGLVHSLFTVQII
                     LFIEKNFQVELEVSELKTEQIATVNKIVELIQRQTGLE"
     gene            13448..14578
                     /gene="clbF"
                     /locus_tag="ACHKUC_000159"
     CDS             13448..14578
                     /gene="clbF"
                     /locus_tag="ACHKUC_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis dehydrogenase ClbF"
                     /protein_id="HFO6616492.1"
                     /translation="MCTENYELAQQEAVLFAKQHLALAAQNIERQQFIVPDIISCVAQ
                     AGYLGASIPQKYGGRGYDSYQLCALHEVMAGVHGSLENLITVTGMVSTLLQRVGSAAQ
                     KAHYLPKLATGELIGAIALTEPNIGSDLVNVETELQQDGDGWRLNGKKKWITLGQIAD
                     FFIVLIHCGNQLATVLIDRNTDGFTITPLNDMLGLRGNMLAELHFNDCRLKEDALLGP
                     LTPGVPLAVNFALNEGRFTTACGSLGLCRAAVDVAARYIRQRKQFKRRLFSHGIVQHL
                     FATMLTQTRSAQLMCFSAAEYRETLHPAMINQILMAKYVASKAAVDVAGKAVQLLGAN
                     GCHADYAVERYYRDAKIMEIIEGTSQIHEIQIAMNYMMGSEA"
     gene            14575..15843
                     /gene="clbG"
                     /locus_tag="ACHKUC_000160"
     CDS             14575..15843
                     /gene="clbG"
                     /locus_tag="ACHKUC_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis acyltransferase ClbG"
                     /protein_id="HFO6616493.1"
                     /translation="MTKDVALMFPGSGSQYVGMARWLYERYPQVRTLFDEASQITERD
                     MAALCLSGTLVQLAEPTAMALAIYTTSVAHFVAWQQFLAQTGVHVNLRYMLGHSLGEY
                     AALTCSGALSFSQALALVAMRSRLASEIAREMDASTTIIKQGNQALVAAACEVAERET
                     RQQVGIACFNSPQQFMLSGQNSAIIAAEQYLLDHDRQVEVVPLIGGVPYHSPLLKPCG
                     QQLRKALDRCEWRRPCCPVISNVNAQPYPDTVTPVQWLEQQLSQPVQWQRSLTYLTGH
                     LSPIAIEIGPQSVLKNLLLENRYPAPVYAFDNRHDRAQLALVLGDNMAVKTDPEAVRR
                     QRITLLTNALTATRHHRAADVAASAQLKELLSRFFERIQQIEQRGTSSEEDIAFLHEL
                     LEQGFQLKGSSQAEIDACHARLASDNGGQA"
     gene            15891..20687
                     /gene="clbH"
                     /locus_tag="ACHKUC_000161"
     CDS             15891..20687
                     /gene="clbH"
                     /locus_tag="ACHKUC_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbH"
                     /protein_id="HFO6616494.1"
                     /translation="MEQQGIMRQLPTDDQTIVDYLYRIAGEYGEKAAVLMGDAALSYH
                     DLNARSNQLAHYLRGLGIGEDRVVAIRLPRGMAMLIAIFAIVKAGGAYLPLAYNAPRS
                     RIENILSNSGAVCLIGTDDGDRWPIPRVEIDSAAVSAMPTTDLRYRPHARQLAYIIYT
                     SGSTGVPKGVATEHAALLNRIVWMQNAYPISSQDVLFQKTVYTFDVSVWEMFWWAMYG
                     ASVVLLPSGLESDPRTLARLIQRHRVSVVHFVPSMLNLFVEYLEMKQDPRLTASLRLV
                     FSSGEKLTVHSVARFYQSVAQGDLINLYGPTEAAIDVSHHRCLRGYDYDDIPIGQAID
                     GCRLYVLDDHGNPVADGEEGELYLAGIGLARGYLNNVALTDRCFTIHPTLRHLGKPER
                     LYKTGDLVWRDGESQQIHYIGRNDFQIKIRGLRVELGEIEAHAMRFPGVQQAVVVADQ
                     DDPDNQLIYAFVVSSVPLNLAALMDALSKNLPAYMLPNRLLAMSELPLSDNGKCCRKT
                     LLDLARAYSASRVDLRETPAVRYLPLSSAQSSMWFMQQLAPHTALYNNPTALLLEGEL
                     DRTRMDGAIRQLMSRHTLLRAMAETHNGQPVLAVPQCVSSQALLTIVPLPSVSDDNAL
                     QAMINQRAAHPMPLTSGTPLCRFELLTLDDDRSVLLIHLHHIISDGWSKGVLLRELQA
                     AYNGESLTPEPLLEYADYMEYQEEWRQSDAYQDAMRYWQNTLAGTLPILDIPTDQPRQ
                     KVARYQGAFVAFALSANTCERVLAAARAQRVSLYNYLLTAFVLLLHRNARQQEYIVGM
                     PIAARLTKEQEHMIAPLVNVLPLRLPLDEAASFSELVQTIRGILFAAFRHQRLEFTDI
                     VRAVNVDRSAGHFPIYQCMFQLDNMPLASPTLNGVNVTPLLLDTSASQVDISLSMQHI
                     DGRITGTFEYDAGLYSADRIQHLVAQWRELLDEASSQPTQLVRDLIRFTPREHAWLAR
                     HNATEVALPPVDNLLALVLPHCQQRPTQVALRHADDAMTYGELQQATMQMCTWLRAQG
                     VKRGESVALQLPFCFELIIAQLAILSLGASYVPLDGNAPAARNALILAQATPCMLLVA
                     QPLESPHGLTIPWVLVPDWRSLLTEIPNLPVSVAPDALDCDAVVIFTSGTTGQPKGVR
                     LSQRNLVNLTASFISSYQVTHQDVLLPITSVASASFVGEVLPLLAAGGTLVLAQKAQS
                     LDSDALIALLASQRVTILSTTPSLSASLSVLAQSMGSLRLFLCGGEALEYEQIAPLLP
                     HMAVVNGYGLTESGICSTYFPVAKRREQETGALPIGRPIQNTQAYVVDAYNRLVPPGA
                     CGELCFSGLGISPGYLDARQDPERFVELPEYPGVRVLKTGDRARWATDGMLFYLGRQD
                     RQVQIRGYRVELGDIESLLKQHPDIADAWVDVRRNAAATPLLVAFYCSVNGVALDAQQ
                     LRVWLSLRLPLHMLPLLYVPLSAMPLGVNGKIDPQCLPLVDLRQLEGPGEYVPPATEL
                     EQRLAEIWQQLLGLERVGTTTNFFDLGGHSLLLVQMQQYIGQQCGQHVALVDLLRFTT
                     IKRLAEFLLAPDAAQGTTGDQTQLRAAKQRLAFGHTRWAATTDSHH"
     gene            20737..23769
                     /gene="clbI"
                     /locus_tag="ACHKUC_000162"
     CDS             20737..23769
                     /gene="clbI"
                     /locus_tag="ACHKUC_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbI"
                     /protein_id="HFO6616495.1"
                     /translation="MAENDFGIAIIGMAGRFPQADTVQAFWENLLASRECISFYSDEE
                     LLAMGISPEFVQHPDYVKAKGEVADIDKFDAAFFGIAPREAELMDPQHRVLLETAWAA
                     FEDAGYVAADYPGDVGIFAGKSMDSYLMLNLMPHFKRVFSSGSLQAAIGNDKDSITTT
                     IAYHLNLRGPAITVQTSSSTSLVAVCVACQSLLTWQCDMAIAGGVTLGPPAKTGYLSQ
                     EGGITAADGHCRAFSDNSSGFVPGTGAGLVVLKRVDEALRDGDNIYAVIKGFAVNNDG
                     SEKISYTAPSVDAQARAIAQAQRLAGLTPQDITYVEAHGTGTRLGDPVEFSALSQAFA
                     GASQKQYCALGSVKTNIGHLDTAAGVAGLIKTALAVQQGIIPATLHFERPNAQIDLTN
                     SPFYINTTCQPWQPESGIRRAGVTSLGMGGTNAHVVLEQAPAVDLQARAPVPAYSILP
                     FSAKTDSALSSGLARFADFLQHESLPDRRDLAWTLSQGRKAFAHRAALVTRDLHAAGT
                     LLQQAATAPFARGVAQTQLGLGLLFSGQGSQYQRMGHQLYQVWPAYADAFDRCATLLE
                     REYQLDIRHELFRAEVSLAQGERLAQTCLTQPLLFSVEYALAQLWLSWGITPTVMIGH
                     SLGEWVAATLAGVFSLEDALRLVARRAELMHQAPSGAMLMVALPEAQIRALITAPLAI
                     AAVNAPDYSVIAGPTSEILAVSQRLTEQNIINKRLHTSHAFHSSMMQDAAQALRQAFE
                     NVRLNPPTLTIISTVTGAHVSADTLTTPDYWIEQMLMPVQFSAALQEAQATFDVDFLE
                     IGPGATLTQLTNGHALGDRLAFSSLPAGARSSDEHKHILDTVAALWVRGHNIDLSAFA
                     GEQPRRVSLPTYAFDKIRYWVDSPEEQRSAVTPVADAGSVIPSEPSVRRQPRPAFSVP
                     YAAPESKTQRGLVAICEALLGIDGLGIDDNFFEAGGHSLMLGMLLAQVQERFAVTLSF
                     FDVMEDASVRALAQLVEQEQQDDGGSALAVLVNDMINE"
     gene            23813..31135
                     /gene="clbJ"
                     /locus_tag="ACHKUC_000163"
     CDS             23813..31135
                     /gene="clbJ"
                     /locus_tag="ACHKUC_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbJ"
                     /protein_id="HFO6616496.1"
                     /translation="MTIHHAALARMLPAEKKEKLLRQLAQSGVSPSRIPIIKADPAQA
                     IPLSFNQERLWFLQKYDSTATNYNLYVVYRLHGVVDMPMLTEALRHVQARHAILRTRI
                     IVRNDRPCQVIDDASSLVLDTVTLAAQAPTSALDAVIQQVINTRFDLARGPLWGVTQI
                     IQPDQGCHLVFCAHHIIIDGISLRLLFDELQQQYARLHAGNETSLPPPPLQYADYAFW
                     QREWFQDTLLANELAYWRARLQDAPLLSTFPSLHPRPAQPSTHGSRFSITLDETLSLA
                     LKHVARTQETTPFVLMLTAFQLVLMRYAQQQRLVIGMPVSGRIRPELQSSIGYYASTA
                     VIYTDFNGVEVGREALQRVKASVKETQGRQQLPFENLVNMLDLPRSLSHSPLFQILYI
                     YHNHVTPRAFTLAGAYWEQVTYHNQTVKYDMTVEVFQNDATFDVSFEYDLGLYDADVV
                     KQIAEALRQHCLSLTSSLETPIGAIPLHAPETATPRRDPLNATNVPWLGPQDVLRIIE
                     QRCVQHPKQLAIQQHDGTLTYAELWARVQFIAMRFRAHGIQPGDRIGVLLPRHRDVIA
                     TMLATWFVGACYVPFDIHQPAARLQRLMQRARLVCLVVRQPGEWGEIVQLSLPELMQD
                     MSNAIRYSTPCALLPDMQAYLLFTSGSTGEPKGVCVVHRGLLNLLLDMQRTFAVGSQD
                     RLLSVTTPTFDISFLEYLLPLISGASLYLTEAERAADSFRMIPLIADYRPTLMQATPS
                     FWHGLLMAGWRGDPELCVLAGGEALPTKVAEELLRCCGSLWNLYGPTETTIWSLKSQI
                     TQAENITLGAPIANTRIYILDNEGHPVPQGVDGELYIAGDGVAQGYDGQPELNAQFFL
                     SEPGVPGGRMFRTGDLVRSDAQGQLFFVGRKDSQIKLRGYRIELGEIERTLARHPHVD
                     AAVVACIERAPLHKALAAFIITSEPPSLFEQLKNELRQQLPDYMVPTLWQRVADFPNT
                     DNGKIDRKRLAENFVADSSLVSPQTQALSDTEQMLLALWMRYLPIKNVDPECDFFRLG
                     GHSLLAVTLVAEINRTFHCALTLKDIFHYSTLRALSARIAQQSITDAAASQDDWVIVH
                     DPEHRHQPFPLTDVQRAYWLGRQTGATSIATHIYHEFDVEHFNVTRFTHAVNALIARH
                     EMLRARVLPDGTQQILAQVPAYQLEQRDLSALSPNARNDALMAIRDRLSHHVHPADRW
                     PLFDFSYSACTAQHGRLHFSLDLLIADALSMRTLQQELMMLYREPHVSLPLLPFSFRD
                     YVQALLVEQASEAYARDQAYWQRALPQLYGPPTLPVQGDLAQLSAIRFVRRRHRLSAH
                     NWGVLSALAQRTRITKTALLLTVFSQVLARWSLSPTFTLNLTLFNRPQGYPNAEAVIG
                     DFTAVSLLNVCYDSQHSYAHNAQRIQVQLWEDLEHRRFSGIRASEALIHSGRFHAPMP
                     VVFTSMLDIDGETTAQDPRDTTRFTLCPDANITQTPQVWLDHQVIELAGELHFNWDAV
                     EQLFDTTLLDQMFGAYCHALQALVAMPQSWWGVNSSLALPTVSAPVTQAPAPTALLHH
                     GLLRQAALTPQETALITPIRELTYRQLSTAADHVARALLALGVQHGDRVAVVMEKGWQ
                     QIAAVHGILRLGAVYLPVDPVLPPQRRQLLLTVGEVRVQVTQPGLTQLEPSLPVLIID
                     DGMLDTPAAPLPEVAGDVTDLAYIIFTSGSTGTPKGVMIDHRAAMNTLEDINERFGLN
                     AQDRVFGLSSLSFDLSVYDAFAPFMVGAALVLPEAGREKDPRHWQTVMAHGHVSVWNA
                     VPALMQMLCEYHSGDRMSYPTLRLALLSGDWIPLTLPEQMRERLNETMDIISLGGATE
                     CAIWSVYYPIGEVESTWTSIPYGRGLRNQPVYVLNAQLEECPVGVEGEICIGGMGLAQ
                     GYLNDAEKTAASFVWREASGERIYRTGDRGRYFADGQVAFLGRNDTQVKVNGYRIELG
                     EIERCIARHPDVEQSVVVAVGNSQHRRLVAFAKLHDRHQAQALQAKEAEAAALAQGII
                     VNPAQRLAFKLKEPHIRALDGLGIALTAPADSTRYIKRRSYRHFSAQKTTLAQLGQLL
                     SGLGQMRLPGLPFAKYAYASAGGLYPVQTYVYLHPDKIEEGVSGIYYFDPRQSCLMPV
                     APEVELNSGFHAGPNQSIADRAAFTLFMVADMAVISPFYGQEAAWHFSVMEAGTLCHL
                     LEEDAPRYGLGLCQLGMADFSAVASHFQLSPHHRYVHCTVGGAIGQEAASAAALLRDF
                     STYEKPKETAAPLDMQSYKDAMLRGLRQQLPDYMVPSDLMLATDFPLTANGKLDRQKL
                     QLQGEQIAHQRDGVGPIQVDSALQQRLVALWQEVLGVSHVSAEDDFFSLGGSSIELVR
                     IQQALEAIIGQEIPIVDLFRLPTIADVARYLDEQLHNLPAAHDIVLAQAEVSQVSAAR
                     ENLALRRKRAQQGEKGDE"
     gene            31128..32591
                     /gene="clbL"
                     /locus_tag="ACHKUC_000164"
     CDS             31128..32591
                     /gene="clbL"
                     /locus_tag="ACHKUC_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis amidase ClbL"
                     /protein_id="HFO6616497.1"
                     /translation="MSEQSYRSAGTLLAQLASGETTSVALVNHYFSRMAQFNKPLNAV
                     VQQHYALALEAAARADRERLEGRARGVLHGLPCTVKESFDVQGWLTTSGAHYLKDNRA
                     TQDAPSIARLRAAGAILMGKTNVPMMTADWQTYNDLYGTTHNLWDRQRSPGGSSGGAA
                     VAVAADFTPVEFGSDLFGSLRIPAHYTGVYAHRCSLGLMSVRGHVPGGGPQATDEPDL
                     STAGPMARSAADLRLMMRALSTFWVEPPRIPDFSRYQAKANYRVCTWFSAPHHEIDQQ
                     IAQRFQSFIDKLRAQPGVEVDDAMPADIDPDALFDIAVKLSGRLVSTALNGRQRLTAG
                     LAALGFRLVGKLADVPEGITSYYQGMLKDSGEQRNTDKLRHEYSRVIETLFARYDVLL
                     TPVSPVLAFAHMQQPVRKRKLIVNGEPQDYNEHLFWNMLATVFGLPATVYPLAKTMDE
                     LPCGIQIISGHFHDDVTINFAEFCESISGGFTVPEGY"
     gene            32653..34092
                     /gene="clbM"
                     /locus_tag="ACHKUC_000165"
     CDS             32653..34092
                     /gene="clbM"
                     /locus_tag="ACHKUC_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precolibactin export MATE transporter ClbM"
                     /protein_id="HFO6616498.1"
                     /translation="MTVNKLEPDNGTPNDELFTVAGMFDGSLYKLLLRMALPMFVGML
                     TQVTYAIADIFWLSHIDVTNSGIIAGVGLVFPVGMGLFAIANGIQIGMGSLLSRAIGM
                     QRLDRAQRILSVGIIIALFFAIVITVLGYVYAQPLLRSLGATKSIIGYATEFYYYSLL
                     TVFSIMLIGVMMGLFQGAGKIMVIMKASLLGALVNIMLDPIMIFVFDFGVKGVALASF
                     LAQLSMVAYFIYTLMGLHIGLSIRIALRPFSWKIYREFLSVGMAQMLMQLIIAVGIVI
                     YNFFIVRLDVNAMAAFTLTGRIDYFIITPMLAIATALLTVVGQNWGHGNVTRTLNAYW
                     AAVALAFSIVLVLAVMHIVLAPWMYPLFTRVVAVSDYAVLQTRIMALALPFVAISLLA
                     SEYYQAIGKPWYSVLLTLMRHVFISVPVVYLLAIVLEMRITGVYFGAMSGTFVAALLA
                     WRLLRLSPRLLRWNQEAVRSQHLDMEVAP"
     gene            34089..38456
                     /gene="clbN"
                     /locus_tag="ACHKUC_000166"
     CDS             34089..38456
                     /gene="clbN"
                     /locus_tag="ACHKUC_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin non-ribosomal peptide synthetase
                     ClbN"
                     /protein_id="HFO6616499.1"
                     /translation="MMSGNPLSWPQEQCHIIDQLYPYSAVNIIGGVVTIEGIVDLPRL
                     HAAIQSAIRQFDALRMWFVMGEESEVVSQVQPYHWRDIRHLTFSPDYDKENLRPAAIE
                     TFVDEWFRQPFTLLAHDLFEFVTFTCGEQYSGYLFKAHHGIADGWSMALLSNHVKRAY
                     EQQDVPDDASPAYSAFLAQQQSYQASTRFAVDRGWWRDYIDEYRDCFPDSSPIVTTEG
                     ISCSTWLEPAMINRLYRLCNRYGCTLNTLFIALFALYRARVWGEEKGVIGVPLANRHT
                     REARRCFGMFTNQLPLAYRLVRTERFCERVAFFQRELKRGFKHSKYPITLFNQDLAEQ
                     GGGKLRAFDYCVNYYNFTYERHIAGAAQRVESYYSGEQSYKLQIVLQTVNNHKESLRL
                     SLEALRSAFTPHQLTAMKNGLLDLVTALDRQPDARLGDLEVYPAPHVALACGSLKPSF
                     TSRFAAQVVEHGDRTALIDNEQSLTYRQLDDAVERVARYLRQQGIGRGQVVGIIAEHS
                     AQTVMVIYGILRCGAAFLPLNPALPTTRLYAMCRKAQVAHILYDPAMHELTQALAFPA
                     SSLLQALATSALAREPWPAIEPQDLAYVLFTSGSTGEPKGVQVSHGNLANYLHFAAER
                     YFTAQDRAALYSSLSFDLTITTLFAPLCVGASISVCRHAESETLLRMAVVDQPNTVIK
                     LTPAHLRLLCAAGISSEQIRTLVVGGEDFKRDLARKAAALFPQAVIYNEYGPTEATVG
                     CMIYRYTGQETLPSLPIGMAIDGCQVAICSPWGCPVPEGETGELVIYGASVTQGYIDA
                     PQQTAAAYLKDTNGVMIGYRSGDIGYAIAPNTLVYQGRKDDQVKINGYRIELCEIEQA
                     LLSAPQVESAAVAVIDDVQGQHSGLLACVTPSSVDVATVMQHLRQQLPTYMQPKQCCA
                     IAQLPLSHNGKVDVRQMVATVRNTVPASGSERLGDAAIRHSVRVCVEGALEQTEFDDN
                     ENLYVLGLDSIKSIQIAAQLRHHGWTMSAVQVMECGTVNAICEFLASHTTVSQLAQYA
                     HNTRIDLPALRWFTQLALPVPNVYNHVIVLKVLPGCPLEQLHNRLHTLIQQQPALHSA
                     LDAEGRLLVCDPNVCYPNEVLTEYSTAQWTLAEVIAQCNSMLDVTNGRVFTAALLHAP
                     QPASSTLVLCAHHLCVDMHSWYLILSTLDAVSTVNGTSNSGLHRWNDYLASKTVDSAT
                     HESWRTVCQTLPLHFPPVSLPDDSLPRTRAWREDFRHPCVRRLFESSGNTAYSAETYV
                     LTALALVLRYYSEEPWCRIEMEGMGRGCWPDEPDVADTVGWFTLFYPWAIPLHGDMAT
                     LLSAIASDLAKRTHGGGDYGLLQMRHAPEDSLAQGIRMNYIGVQAQPSLRYFHIDHFN
                     SDIYTAPENALGCVLEFNIARSAADGLSFHCRFDPTRIALNDVQLLLARYKNSLTDLD
                     AWLCQHSATLTGAPTLWTL"
     gene            38487..40946
                     /gene="clbO"
                     /locus_tag="ACHKUC_000167"
     CDS             38487..40946
                     /gene="clbO"
                     /locus_tag="ACHKUC_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin polyketide synthase ClbO"
                     /protein_id="HFO6616500.1"
                     /translation="MAKDDFTCGSLDIAIIGMSGRFSGAESVPEWWDKLLAGEEFTQP
                     TCTEDDNGNPWIRLRNIITAPYDFDAAFFNIPPGEALLMDPQQRIFLECCYNALEHAG
                     YIPTQLKRVGVYGATYANNYFIDRVYPYLKMSGDHHYLQAQIGNEKDYLCAQVAYKLG
                     FTGPAVSVQTACSSSLVAAYLACEGLLTFQADVALAGGVTLGFLQAHGYSPQGDKLVS
                     QDGHCAPFSAEATGTVYSSGAGVVVLKRLEDALRDQDRVYAVIKGGAVNNDGGRRLGF
                     VAPSVEGQVEAINTALAAAEVVPTDIALIETHGTGTPLGDEIELEALHRVFAPACAPH
                     SIQLGAVKANLGHLGVASGIVSLMKTALTLYTGLVPPQINLVNKHKKLLQPASPFYLS
                     DVVTSVPQTKRIHATVSSFGLGGTNAHLVLQNWCETPAQAVQENERRLFFFSAKTPLA
                     LRQQLDAHYHALATYAEADKDRIAYTLAQRRAHFPYRCALAADSVVALRASLAKLRDA
                     DMSFTPINMETTLVFLYPDRDDKLESALTHLLACQPNLRQRHQRLSQDVAQICEPADW
                     TPALRQFIQQVSLSEWLIEQSISPVQHIGYLTGAAAAQYVARIISLENAVQQVIVAET
                     TPEQTLAGNSELSEILANLAVTEGTLMLEIGRAGTFSILYHQHAQWVGQTVFSPMLNT
                     DTPEDILPLLGTLWQRGVTICLPEMPAVQTIGLPGYSFDRVRYEIQSSDARENAMLPV
                     SYLSVSDFVEKTWRSLLCIDHYDEHAVIFEYGATSMHVISFVDSCNHIYKIGLTAADI
                     YARPAIREHSEFISECVDGIL"
     gene            40959..42473
                     /gene="clbP"
                     /locus_tag="ACHKUC_000168"
     CDS             40959..42473
                     /gene="clbP"
                     /locus_tag="ACHKUC_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precolibactin peptidase ClbP"
                     /protein_id="HFO6616501.1"
                     /translation="MTIMEHVSIKTLYHLLCCMLLFISAMCALAQEHEPIGAQDERLS
                     TLIHQRMQEAKVPALSVSVTIKGVRQRFVYGVADVASQKANTLDTVYELGSMSKAFTG
                     LVVQILIQEGRLRQGDDIITYLPEMRLNYQGKPASLTVADFLYHTSGLPFSTLARLEN
                     PMPGSAVAQQLRNENLLFAPGAKFSYASANYDVLGAVIENVTGKTFTEVIAERLTQPL
                     GMSATVAVKGDEIIVNKASGYKLGFGKPVLFHAPLARNHVPAAYIHSTLPDMEIWIDA
                     WLHRKALPATLREAMSNSWRGNSDVPLAADNRILYASGWFIDQNQGPYISHGGQNPNF
                     SSCIALRPDQQIGIVALANMNSNLILQLCADIDNYLRIGKYADGAGDAITATDTLFVY
                     LTLLLCFWGAVVVVRGAFRVYRATAHGPGKQQRLRLRVRDYIIALAVPGLVAAMLYVA
                     PGILSPGLDWRFILVWGPSSVLAIPFGIILLAFVLTLNHQIKRILLHNKEWDDE"
     gene            42466..43188
                     /gene="clbQ"
                     /locus_tag="ACHKUC_000169"
     CDS             42466..43188
                     /gene="clbQ"
                     /locus_tag="ACHKUC_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HFO6616502.1"
                     /translation="MSNISLYCLPYSGGSAAMYYKWRSVLSDNITLRPLEPAGRGTRI
                     RQPLCLTMVDAVADLYQQFVKHYTGGDYAIFGHSLGGIMAFELVHYILDHGHDMPCAL
                     FFSGCRPPDRASHEVILHTLPDQAFMEEIVKLGGTPVDVFRNKELMTIFTPIIKNDYR
                     LYEQYVFQAKARTLTCPIVLFHGDADNLVMQDELLAWEKFTTRKTRTIIFPAADHFFV
                     DKHFEQVVGYVNQTIESLEIVG"
     gene            43223..43735
                     /gene="clbS"
                     /locus_tag="ACHKUC_000170"
     CDS             43223..43735
                     /gene="clbS"
                     /locus_tag="ACHKUC_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HFO6616503.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDELYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(44015..45286)
                     /locus_tag="ACHKUC_000171"
     CDS             complement(44015..45286)
                     /locus_tag="ACHKUC_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6616504.1"
                     /translation="MSLNDAKIRSLKPTDKPFKVSDSHGLYLLVKPGGSRLWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGIRKMLALNINPAQQRAAERGSRMQEKMFKSVA
                     LEWHSSKKKWSQNTADRVLARLNRHVFPTIGHLPVTELKSRHFIELLKGIEEKGLLEV
                     ASRSRQHLSNIMRYAVHQGLIEINPAANLDGVTASPARRHYPTLPLERLPELLERIDS
                     YHQGRELTRLAVLLTLHVFIRSSELRYARWTEINFRNRIWTIPATREAIAGVRYSSRG
                     AKMRTPHIVPLSEQVISILKRIKEISGGYELVFPGYHDPYKPMSENTINKALRQMGYN
                     TKQDICSHGFRAMACSALMESGLWSQDAVERQMSHQERNTVRLAYIHKAEHMEARMDM
                     MQWWSDYLDMCSEIWVPPYIWSQQNINLAVT"
BASE COUNT        10151 a        11559 c        12812 g        10920 t
ORIGIN      
        1 taaatccgat aataaggtga tggttatgga taatacctct ggagattttc catgtaataa
       61 gatggacacg cgtaagcagt taccgctaac accaagtcaa caggggtttt tattccattc
      121 cttaaaggat aagaaaagga gtaactacca tgagcatttt acatgcattt tttctcagca
      181 tgtagatagc gcccacttca agtgggcgct ggaaacgtta tttcgaaagc atgagtgttt
      241 tcgcactgat tataactggg agattgatga gcgcccttgt caggtggtga agaccgatgt
      301 gttgccggat atatatgtgt tagactgtga gcaagaggaa atacgttttc tactagctaa
      361 tgatgacatt atcattcctg tcccgcagga tgacggtatt gatgctataa ttcctcaact
      421 gctacaggct gatttaaaat acccattttc cttgaaaacg atcccagtcc gggcctacct
      481 tattcagtca acgaaagaaa gtgcttttat actatcatac catcatattg tgatggatgg
      541 ctggagctta tcccttttca ttaaacagtt gctccaactc tatggagcgg ctgtggtcag
      601 tggtgtgagg gatgatagcg ccattatccc ctcatctctg aaaccccttg tagacacact
      661 gtcggcccga cgtcacacct ttcagcacga ctattgggct gcatatcttc gggagggaac
      721 accaacttgt atcgtgccgc tgtcacaata tcacacagat actgaagccg agaacaattc
      781 ttacgttaat caaacaaatc atgtggagat caatttgtct ccggatgtgt gtcagaaaat
      841 acagacgcta tgcagcgatt atcgtatcac ccccgcagta atcttctatg tggcctgggg
      901 catcctgcta caacgttggt gctatgctga cgatgtgtta ttcggcgcga caatatcagg
      961 gcgaaatata ccaattgatg gtatagaaga aacactaggg ctatttatta acacgttgcc
     1021 gctgcgtctg cgtgatgatg gggcgacact gttgcaacac ttacaacgta tgcaccaaac
     1081 actaatagct cactacagca atgaacatga tgccttagcc agcatacaac ggttggtaca
     1141 taaagaaggt catgctgggg atctttttaa taccttagtg gtgttggaga attatcccgt
     1201 tgatatgaca ttattgtcat gcgcgtcgcc cgtggcaatt cgccatctca gtgtacatga
     1261 acaaacgcac tatccgctga ccttgaccat cactcaacag aagggattcc gtttcagtat
     1321 tgcatatgcc cttaactacc tgaccaacaa catggcgcaa gcgttgctga tgcacctgag
     1381 ttatctgctt gaacaactgg tggacaatcc gcagcgcccc attgctgcgt tggtaaactt
     1441 gtccccatgc cagcaggcgc aggtacttca accctatctg gaacgcatgg catgccggga
     1501 ttgggatagt caatccaacg tcatcgaaca atttcatcaa gttgctgcga cttcgccagc
     1561 acaggtcgca gtggttgatg aattgtgcgc gttgacctat tcggagttgg cagcacaagc
     1621 tgagcagctc gcggcttatt tggtacagca gggtgttatg gttggcgata ctgtaggcat
     1681 aattagcgaa cgccgggtaa acaccgtggt tgccatcatc gccatcatgt tgatcggggc
     1741 tgcttatgtg cccatcagcc ccgactaccc agtgggtagg atgcaggaaa ttattgatga
     1801 cagcggcttg gcgctgttgc tggtacatgg caaaccgcta gatgcattaa acgttgcgca
     1861 gagtgacctc tgtgcatttc ccgtcgcgcc ctcggtggta tttccggtta tcacaccaga
     1921 ttctcgcgct tatgtgattt attcatcggg gtcgacgggt aagccaaaag gtatcgcggt
     1981 ggcgcaccgc ggtttattgc gcctgataca aggcgacagc ccgctgaagg tggagagcgg
     2041 tgagacaacg ctgctgacct gcccatttga gtttgatgtt tcggtgtttg agatgtggtc
     2101 taccttgctc aaccacggca aactagtatt actcagcaaa caggcattac ttgatatcaa
     2161 tcacattcgc cgcacgatcg ctgatgaaca ggtggcgcgc gcctggttta cctcatcctt
     2221 gtttaactcc tatgtggcag aaggtgccga tttcttcggt atgttacaac acattacggt
     2281 gggcggcgaa gcagtatctg cgtggcatgt caacgacgtg atgcaaaaat acccgcatct
     2341 ggtggtgaca aacggttatg ggccgacgga aaacactatt tttaccaccg catatcgttt
     2401 caacgggttg caacccgccc gagtcccgat aggatacgcg gtaccgggca cctcgctcta
     2461 cattaccgat ctccatgggc atttgttgcc tatcggtgcc accggtgaac tagtggcggg
     2521 tggagtgggg gtcgccatcg gctatcagaa caacccggcg ctaagtgcga cggtttttgt
     2581 ccctgatcct tttattcccg gcggtatgat gtacaaaact ggcgattatg cccggttgct
     2641 ggatgatggc tgtgttgact gttttgggcg taaagacggt caaattaaga tcaatggaca
     2701 acgaattgaa accggagaga tcgagcagcg cctgctggag tgctccggca ttatcgaggc
     2761 ggtagtggtt ccttaccgcg tacgtgaaac gctgcatatt gcggcagtgg tctgcgtcaa
     2821 tgatagctat gatgaggtgg aagttcgagg gcaattggct gacagattgc cgccattcgc
     2881 tattccggaa tccttggtgg tggtgacgga gattgcaaaa agccatagtg gcaaggccga
     2941 cttggcgcag ttgcggtatc tcctgcccgc aactcagtgc aacgccgtgt ccaccacgat
     3001 atcagaggtg catagtgaca tggaacatgc gctgcatgct atctggcaac gcgtacttga
     3061 tagacaagac attgatagca atgcctcctt tttcgccctc ggtggcacct cattggatac
     3121 catcagggtt aaaggggata ttaagcggca acttggcttg gagattgata ttaccgatct
     3181 ctttaagtac ccaacgctca cggcgttagc acattttctc gatactgccg tatcgccgga
     3241 ggatgcaatt ccaacgcgtg ctgttgtcta cagcgacatg ccggtggcga ttgtcggtat
     3301 ggcgggacgt ttccccggtg cggcgaatat tgcagcgttg tggacgctgg ttgtaggagg
     3361 ggaatcggga ttaacactgt tcagtgatga agagttgcgc gcgcatggtg tgacacctga
     3421 cacgcttaaa caagcgaatt atataaaaac caaagggatt gttgatgatc acgaatggtt
     3481 tgatgcggat ttctttggtt atactcccaa cgaggcggaa tgtatggatc cgcaaattcg
     3541 cttattgcat cagtgctgct ggcaaactct ggaacacgct gggtgcgatc ctgccacctt
     3601 tactggtgcg attggcattt atgccggact gctgacatcc ccccactggc ttaatgcggt
     3661 aatgcaagac actaccgact ctaccgccct gtacaaggcc agtatcctga atatccattc
     3721 cgtcacagca ttgattgccc atgcgcttaa cctcaccggc cctgccgtga cgcttgacac
     3781 cgcctgctcc acttcggcag tggctatcca ccaggcctgc atcgcactac gtaaccggga
     3841 ttgcgatgcg gcattggcgg gcggcgtttc tatcgagatg cctgcgtacc ggggctatga
     3901 atatcatgaa ggcatgatca atgcgcgaga tggtgtctgt cgtcctttcg atagccaagc
     3961 ctctggcaca gtcaccggtg atggcttagg aatgctgctg ctaaaacgtc tggacgacgc
     4021 gctggcggat cgggactgca tctatggtgt gatcaaaggt tcggcggtca ataatgatgg
     4081 caataacaaa atcggctata ccgcgccgag tgtgatcggg caatcaacgg taatccgcac
     4141 cagtttgcgc cgtgccggtt ttgatagcga cagtatcgga ttggtggaag cgcacggcac
     4201 cggtacggtg ctgggtgatc ctattgagtt acgtgcgctt aatgaggtgt ttggcccaac
     4261 acctgttccg ttttgtgtgg tttcagcgct gaaaagtaat attggccacc tcaactccgc
     4321 tgcgggagtg gcgggcgtca tcaaaacaac gctcgccctg catcatcaag tgttgccacc
     4381 aacagcgcac tttcgccaac tcaatcctgc tatagatcta tcgcgttctg cattgtacgt
     4441 taaccagcaa gtccaaccgt ggccgtcgac gcgtcctcgt cgtgcactgg tgagctcctt
     4501 cggcatcggc ggcaccaatg ccagcatcgc actagaagca catcaacatg aggacgaccc
     4561 ttcagcgacg ggggtacgcg acagctatct gttgttgttc tccgctaaaa cacccgctgc
     4621 gttagagttg cgcgtggcct ccacactgga atatgtcaag catggagtag gggtgcgcct
     4681 gccggatgtg gcttatacat tgcaaactgg acgcacagcc tttgaccatc ggcgggctta
     4741 tttggtgagt cgtgggtcga aaatcgatct ctcctgtgcc acgatattgc aagcggaaat
     4801 cttcaatggt cagcgcacga cagcggagat ctgcttcatg tttcctggtc aaggtagcca
     4861 atatcacggc atggccagcg cgctctatgc tcatcaaccc atgtttcgcc agcacatgga
     4921 tcgctgcttt gctgcattcc aacgctattc gacggtcgat ctcaaggcgt tgttgtttga
     4981 cgatgaggat acgcgggata ttgatcaaac gcagttcaca caaccggcgt tgttctgtgt
     5041 cgaatacagc ctagcgcgca ccttgattga tctggggatt acgccggaca gtatgatcgg
     5101 gcacagtctg ggcgagtatg ttgcggcctg tattgctggc gtatttactc ttgaggatgc
     5161 gctgcacgtc attgaggcgc gcggacgttt gatgcagtcc atgcgtcccg gtagcatgat
     5221 ggcggtctac cttagtcgcg aacagttgac cccatggcta gctgcagaac ggggtattga
     5281 actggcagct aataatagcg cgcatttttg cgtagtcgcg ggcgagcagg cggccatttc
     5341 ccgtttgagc acacgcttag tcgagggcgg gatacagcac aggcgcctga aaacctctca
     5401 cgcattccac tcggccatga tgacgccgat gctgcacgat tttgcacagt tgctggggca
     5461 aatcccgatg cacgcgccgc acaagcgctt tatatctaat gtaagcggta catggattac
     5521 tgaggagcaa gctacctcgc cggattactg ggtgcagcaa gtgcgcaacg cggtgctgtt
     5581 tagcgaaggt gcggcgcaac tgttggtaca acccacgctg tttatcgaat gtgggccggg
     5641 taatacgctc tctaccttta ttcaaggaca taaccaatac agcgatcagc cgaccctgtt
     5701 gacgctacgc aaagccaacg cggcgatcga tgatgagcac atgttgcatc gtacgctggc
     5761 ggcgctgtgg gtcagggggg agaatattga ttggagacgc tttaaccaga cggcactcgg
     5821 caagcacatt ccattgccgg attacccctt cgaacagact tattactatc gctatggtgc
     5881 tgcactttcc ggttatcgcc agtatccaaa tcctctgcgc cgtccgcaag atgagtggct
     5941 ccagcgtgtg ctgtggcgga tgcacgacac atccttgcgg gaggcgttct atgcgccggg
     6001 cgaattgatc atcattatta gtgctgacgg cgacaagtta cagcagacgc tgatgagtag
     6061 tggtgtcgac agcatcacaa tgccgctgcc gatatcgtca gaggacgacg tgtgggataa
     6121 tgaccgtatt ctgacgcatt tccacgacat ctgcgcactt ttagcacaca aaacctaccg
     6181 acagttacat tgcttgtatg ctcccggtgc ggaggcagga tcatcgttga cacagtcgct
     6241 ctcgggactt tatcgtgttg ctcgctggtg tatgcacagc accacgccgc tggcgtcctt
     6301 aacagtattg acccatggtg cgtttcgcgt acaggaagag gataatcccg aaccgacgct
     6361 ggcagcgttg tcgggcgcag taaacgtgtt tgcccaagag ctgcacccga ccgaagtacg
     6421 attgatcgat atcgatgcgc agagcagtga tgagaatctg aacttgctaa cccagcgtct
     6481 ggccccgaaa caagaaacgg taatggcgct caggcagggg atgctttacc tgcgacgctt
     6541 tatcccgaca cggctgttgg cgcacttacc ccctcaaaca gggtgtatac cgggcaacgt
     6601 actgtggatt atcggcggtg aaaaggggat tggccgcatg atcggcgaag cgcttgctca
     6661 gcgtgaggga gtccgtgtgg tactgagcag ccgcacaggt tatcaccatg aagcggtgca
     6721 gcaggacgca ttagacgtta tccactgcga cgtgacgcag gcggaggcgg ttagagcttg
     6781 tctggcaact ctgctcgaac gctatggacg gttggatggc gtgatttttg ctgccgacgc
     6841 taccaccaca ttgacactgc atcaattgag cgaatctgcg ctacgcgaca cgctaacggt
     6901 gaaagaacgg ggtactgcta atgtgctgca tgcgttagcg caacggaatc tgctggatga
     6961 gcgtctgcta ctgctgttct gtaactcgtt ggctgccgtg aatgcggaga ttggccagac
     7021 aggctatgct accgccagcg cctatttgga tgcactggca cagcaactgc gtacccgcta
     7081 caaggtgaat gcgctcagta tcgggttgga tgcattgcgt gagcagggca tgttgttgga
     7141 tgctataaac ggcagtgaat acgatgtctt gcgtggactg cgcccattga tgacgggaac
     7201 gttgctacag gcttataaac aacaaggggc tgacaccagc tactacgcgc gattatcccc
     7261 cgagtccgat tggctgctgg atgagcaccg gatatccggt atcgccacgc tgccgggaac
     7321 cggctatctg gcgctggcgt atgaagctct gcgccattac tttgtgcaag accaaatctg
     7381 cattgatgaa ttggtctttt tggcaccgtt gactgtgatg gacaactgca gtgttgacgt
     7441 ttttgttgac atttcaccta acgggcaagg agttagtgtc gaggtgaaat caatgacgga
     7501 gcgctttagt ggcacgttaa caacccatgc cagaggcagg gcgacgcgtc tgatggtaga
     7561 cgataatgtt gtgtgcgatc tcacggggct gatgcgcgag atgcacacta tcactcctcc
     7621 aacaaaggaa ttatcgagca cgcacttcca ctatgggccg cgctggcaca gcgtacaaca
     7681 actgtatggc aataccgccc agactcaggt tttcgcaacg ctggccctgc ccaccgttgc
     7741 cgctaatgat acgatcgcac tgcaccctgc gctgttggac atcgccagca gtgttgtcga
     7801 acaactgcct ggttttcata ctgattcggt acctttcctt tatcaggatt tacgcctgta
     7861 ccgcccgttg ccgaacacct tacatgtggc gctgactgtc aatcggcacg atgaggaggg
     7921 tgacagctac gctttcacgc tctacgacat ggcaggcgag atggttgccc gctgtgcggc
     7981 aatggtgaag cgcaaggtac agctccacat acaagatgtc gatgacgaca cgcgactgcg
     8041 cgtgcccagt gccgataact accaactgcg gctggccgct gagggggagg gggcaggaaa
     8101 gctagcgttg tgccctacgc cgcgcttagc gctgggggat tcacaagtag agattgaggt
     8161 actggccacc ggactgaact ttaaggatgt gctgttcacc acgggattgc tccggcagca
     8221 gccgggtgag gctccgctgc aattgggact ggagtgcgcc ggacgcatta ctcgcgtggg
     8281 taaaaatgtc actgaatttg ccccgggaga ggatgttatg gcggtgctaa acggtggttt
     8341 tgtccagtac gcacgggtag aaagcgattg cgtagtgaga aaaccagccc attgtcgcat
     8401 cgaacaggcg gctgcgctgc ctatcgcata cctcaccgcc tattacgcac tggtggtgcg
     8461 cgctaatttg caacccggag aacgagtatt gatccacagt gcggcggggg gcgttggctt
     8521 ggctgcgcta catattgcca aacgctgcgg agcacagatt ttcgccacag caggtagcga
     8581 gcagaaacgc gattacttgc tttcgctagg cgtacatgcc gtagctgatt cacacgacga
     8641 acagttcgct gccactctgc tgaccgcatc ggacggacag gggatggatg tgatccttaa
     8701 ctccctcaca ggccgtctgc ttgacgctag cctcgcgctg ctggcaccgc tgggccgttt
     8761 tcttgagctg ggcagcaagg acattgtgga agacaaagcg ctaccgatgc gtttcttcgc
     8821 ccaaggcggc acctttattc cgattaattt tcacgcggcg catggtgcgt ttagtcgcta
     8881 cctgcaacag attgtcgctt ggatagatga taacacgcta ccgctccttc catgcaaatc
     8941 cgtaccattg cccgaggttg cacgcgcctt cgccaccctg accacgccgc agcatattgg
     9001 caaggtggtg gtaacgcatc gcactgcggc aggcatggac cggctgaacg ccatgatagc
     9061 agaaaggcgc ctcggcggct atgcgctcag catgagcaat gccgaggtga tgcgccaatt
     9121 gtggccgata ctgaacactc gcagtccgtg ggcgcaactg ctgctctcac ctcgggcgat
     9181 cgatagatta gcgcggggca accgggtgga tcgcggtgta ccgtctgccg ctaacgatac
     9241 gattactcag cagacagtga aaaagcggcc ccgtccggaa attggcgtgc cttacagccc
     9301 cgcgacacgt gaagtggaac gcgtgctctg ccaaatcctg gaagagtatc tgggtctgga
     9361 tagagtaggc attgacgaca actatgccga attaggggca acctcactcg atatggtgca
     9421 actgagtggg caaatggcgc gtcactatcc gcaagtgagc gtggtgtcgc tgtacaacca
     9481 cgccaccgtg cgccagctgg cgacgttttg ccagcccccg gagggcgagt caaatgcgcc
     9541 atcaccacag cctgcagtac agacgaatac gcgcgcgaat cagatagcaa aacgtgcttt
     9601 gcaaattgcg aaaaatactg cgcgcagtca cacgtctttg cattaatccg ggcgcctcct
     9661 cgccctgaag aaaggtaatg aagtttatgg aatacgcaag cgaaatgaac ggcatggaaa
     9721 tcgccattat tggtatggcg gtccgtttcc cgcagtcccg gacgttacac gaattctggc
     9781 ataacatcgt tcagggtaaa gagtgcgtca ccttctttag cgaggaggag ttgctggccg
     9841 aaggcgtcga acagagtact ctggacaacc cggcctatgt acgggccaag ccctatatcg
     9901 agggcatatg cgactttgat gctgcatttt tcggttacag tcacaaagag gcgcagactc
     9961 tggatccgaa atcccgcgta ttacatgaag ttgcctacca tgcgctggaa gatgcaggct
    10021 atgcccaacg taccagcgat ctgatcaccg gggtattcgt gggggcgtca gaagatgtgg
    10081 attggctacg gcgttcgctg tcacagattg gcggcgatgc gctgaatcgt tttgagtctg
    10141 gcatctatgg tcataaggat ctgctcgcac atctcattgc ctacagtctc aatctcaacg
    10201 gtccggtgta tagtctctac accagttgtt cgacttctct gagtgcaacg catatcgctt
    10261 gccgcagctt gttgtttggc gaatgtgatc tggcgttggc gggtggaatt actatcgatt
    10321 taccgcagaa gtcaggctac ttctgtcaac agggcatgat ccactccacc gatggccact
    10381 gccgtccttt tgacagtcag gcttctggca ccctgtttgg cgatggcgcg ggtgtggttg
    10441 tgcttcggcg tttggaagat gctctggcag cgggcgatcg catctatgcg gtgatccggg
    10501 gtagcgcggt caacaatgac ggtaaacaaa aaatcggttt tgtcgcgcct ggtcatgagg
    10561 gacagaaggc ggtcatttgt gcggcctgtc atctggcaga agtaagccca gaaagcatcg
    10621 gctacgttga aacccacggt accggcaccc gtattggcga tcctatcgag ttcgctgcgt
    10681 tgacggaggc gttcgatact tcacaccgcc aatactgtgc actaggggct gtaaaggcga
    10741 atattggcca cacccacgcg gcggcgggcg tggctgggtt aatcaagacg gcgctggtac
    10801 ttcatcaccg gaccattccg ccgctcgcca actatcaaat gcctaactcg aagctggatc
    10861 tggcgcattc accgttttat atcccgatac agccgcagga gtggcccgca tcgcggatgc
    10921 cgccccgcgc tggcgtcagt tcttttggca ttggcggtac taatgtgcat atgattttgg
    10981 aagggctgaa tcctgcggtg cgcgatgacc atgaccaagt gcgagcaccg gtgtttatcc
    11041 ctctctccgc gccgtctttc gagcagttgg atgagctgac gcaacagctt accccgttgc
    11101 tggctaccct tgatgcgtca acgctagcct acacacaaca agtggcgcgc cccgtgtttg
    11161 attgccgccg agtgatacaa gtggaaaacg atggtacgca agcgatgctg gcatcgctgg
    11221 ataacctaat gcccgacgct ccttggggcc tacactgtcc agatctacgt actacgaacg
    11281 attgtactta cgcacagtgg ctggcgcatt cggcacatta tcaacgcgaa gcgactgctc
    11341 tgacggcatt actcgacggc atgaatattc cacccgctta ttgccacgct gaaacgtggg
    11401 cggcacaagc gaacagcagc ctgctaatca gaggctgcca gactatcgcc gcgctaaaaa
    11461 cctggatgaa cctgctaccg acattaacac tgctgtcggg tgctggaaca ggccttttgc
    11521 ctgccgctgc agccagtggc atgattgcga cgcaagacgt gttgcatttg ctgtgggaaa
    11581 tggagcaaaa ggcgcttcat ctctggcttc ctgagcgcca tgaacctatc cccggctacg
    11641 tgctggcttg gcaaggaaat cccatcaccg atgcacagcg taacgacaga gggttttgga
    11701 gtgaagcgct gttggcagat acccgagaac tgggggaggg cgttcacagt atcaactggg
    11761 ttaggctgcc gccggaaata agagaagacg ttgatgtatt gcgctacgtg gcgcaactgt
    11821 ggtgtgcagg tatcaatgtg gattgggccg tgtggtacgg cactccgctg ccgcaacgcg
    11881 ggagcgcatc agcatatccc ttcgcacaca accactaccc attgcctgga cgggtaatgg
    11941 gtagtgtgga gacccaaccc gaagcaggac ccgaaacgca ccacccttat caggcacgtc
    12001 ccgtgttaag cgtacctttc gtagccgcac actcgcgggg tatgcagtat atcacaggtc
    12061 tgatggaact gttgctggaa atatcgccgg tcggggtgga cgacgacttt tttgaattag
    12121 gcgggcattc gctgctggtg acgcaattga cctcccgttt agaacgcgat ttcaacgtac
    12181 atatcgatct tttgaccctg atggaaaacc ccaatccgcg caatatctac gcgcatatcg
    12241 cggcgcaact ggggggcgaa gacaacctcg aaatagcctg tcagtaaagg agctcaatga
    12301 tgaacgtggc ggtaataggt gcaggagtaa tgggaactgg cgtcgctcat aacatggcgc
    12361 aatacggcat atcaactaac gttgtggata tttctcaatc tcagttggat aaatgccgac
    12421 agatgatcga agcgaactta cgcttatata attttcaccc gcagcataaa aaaaagacgc
    12481 atagcacagc ggagataatg gaaaatatcc gtttcacaac tgagttggac gacattgtag
    12541 aatgtgattt ggtgattgag aatattactg aggatataga gaaaaaaaat gcactataca
    12601 cacggatgaa tacgatctgc ggcgcgtcga cagtgtttgg tgttaataca tccgccatat
    12661 ctatcactgc gttgtcaaaa ttaatgcgac atccggagaa tgtagtcggc gtccatttta
    12721 tgaacccggt accgctgatg cacaccgtcg aattaatccg tggtgtacat actgcagagc
    12781 gtacactgaa catatttcac cacttatttg ctcagttgaa taaaaccggc atcgtggtca
    12841 atgattctcc gggctttgtc actaatcgtg ccatgatgat ttttgttaac gaagccatat
    12901 ttatggtgca ggagcagatc gcccgtgctg aggatatcga tacgttgttt aaaacctgct
    12961 tcgggcacaa gatggggcca ctacaaactg ccgatttgat tggcttggat acgattttgc
    13021 aatcgttgca ggtgctatat gaaagtttta atgatgataa gtatcgcccc agctttttat
    13081 taaagaaaat ggtcgatgca gggtatttgg gcgtgaaatc agggcagggg ttttatcgct
    13141 atcaacagac gtacgccgag cagtgattct gggttaacca gatagggata tgaatatgaa
    13201 aaagcaagat atgaaagccg ccattcggga atttctttca cgctcattac gtgggcatac
    13261 gttgaacgat gatgacgata ttttttctct cgggcttgtc cattcgttat ttactgtgca
    13321 aatcatactg tttatagaaa aaaattttca ggttgagctg gaagtgagtg agttgaaaac
    13381 agaacagatt gctaccgtca ataaaatagt ggagctcatt cagcgacaaa caggcctgga
    13441 gtaaactatg tgcactgaaa attatgagct ggctcagcaa gaggcagtcc tgtttgccaa
    13501 acaacatctc gccctggctg cacagaatat tgaacgtcag cagtttattg tgccagacat
    13561 tatttcttgc gtggcgcagg ccgggtactt aggtgcgtca atcccccaaa aatatggcgg
    13621 acgaggttac gattcttatc aactgtgcgc tttgcatgaa gtgatggctg gggttcacgg
    13681 ttcgttagaa aatctcataa cagtgactgg catggtcagc acgctgctgc aacgcgtggg
    13741 cagcgcagcg caaaaagccc attatttgcc aaagctggca acgggggaat tgatcggcgc
    13801 gattgccctc acagagccga atatcggcag tgacttagtg aacgtggaaa cagaattaca
    13861 gcaggatggc gacggttggc ggctgaacgg gaaaaagaaa tggatcacgc tagggcagat
    13921 tgctgatttt tttattgttt taatccactg tggtaatcaa ttagcgacag tgcttatcga
    13981 tcgcaatacc gacggcttta ctatcactcc actcaacgac atgttggggt tacgcggcaa
    14041 tatgttggca gagctgcatt ttaatgactg ccgcctgaag gaggatgcat tgctcggtcc
    14101 gctcacccct ggggtaccgc tggcggtgaa ttttgcgctc aatgaagggc ggttcaccac
    14161 cgcttgtggc agcctaggat tatgtcgggc cgctgtggat gtggcagcgc gctacatccg
    14221 ccagcgtaaa cagttcaagc ggcggttatt ttctcatggc attgttcagc acttgtttgc
    14281 cacaatgctg acccaaacgc gcagtgcgca actaatgtgc ttcagcgcgg cggaataccg
    14341 cgaaacgctg cacccggcca tgataaacca aatcctgatg gcgaagtatg tcgcttccaa
    14401 agctgcggtg gacgtggccg ggaaggcggt gcaactgctc ggtgcgaatg gttgccatgc
    14461 cgattatgcc gtcgaacgtt actaccgcga cgccaaaatc atggaaatca tcgaagggac
    14521 atcgcaaatt catgaaatcc aaattgcgat gaattacatg atggggagtg aagcatgacg
    14581 aaggatgtcg cactgatgtt ccctggctcc ggttcgcaat atgtaggcat ggcacggtgg
    14641 ctgtatgagc gttatccgca ggtgcgtact ctgtttgatg aagctagcca gatcacggaa
    14701 cgggacatgg cagcgctgtg cctgtcgggt actttggtac aacttgctga gccgacggca
    14761 atggcgctgg ctatctatac caccagcgtg gcccactttg tcgcgtggca gcagtttttg
    14821 gcgcaaaccg gtgtccatgt caacctacgt tatatgttgg gtcatagttt gggcgaatat
    14881 gctgcgctga cctgtagtgg tgcgctgagc ttttcccaag cactggcgct ggtggcgatg
    14941 cgtagccgtt tagccagtga gatcgcccgc gaaatggacg cgagtaccac tatcatcaag
    15001 caagggaatc aggcactggt ggcagcggcg tgcgaggtgg cggagcgtga aacccgacag
    15061 caggtcggta ttgcctgctt caattcgccg caacagttta tgttatccgg acaaaactcg
    15121 gccattatcg ccgccgagca atacctgctg gatcatgatc gccaagtcga agtagtgccg
    15181 ctgattggtg gtgttcctta ccacagccca ctattgaaac cttgcggaca gcaattgcgc
    15241 aaggcgctgg atcgctgcga atggcgacgt ccgtgctgcc cagtgatctc caatgtcaac
    15301 gctcaaccct atcccgatac ggtcacgcca gtccagtggc tagaacaaca gctctctcag
    15361 ccagtgcagt ggcagcgctc tctcacctac ctgacaggac acttgtcacc gatcgcgata
    15421 gagatcggtc cgcaaagtgt gctgaaaaac ctgctgctgg agaatcgcta tccagcacca
    15481 gtttacgcat ttgacaatcg tcatgatcgt gcgcaactag cattggtgct gggggataac
    15541 atggcggtga agaccgatcc ggaagccgtg cgccgccaac gcatcacgct gctcactaac
    15601 gccctgacag ccacacgcca tcaccgcgcc gccgatgtgg ctgccagtgc gcaattgaaa
    15661 gaattgctca gtcgtttttt tgagcgcatc cagcagattg agcagcgcgg cacatccagc
    15721 gaagaggaca ttgctttttt gcatgagctt cttgaacagg gatttcaact caaaggcagt
    15781 agtcaggcag aaatcgacgc ctgccacgcg cggttggctt ccgacaacgg cggacaggcg
    15841 taacccgtca cgccatttta cgtacttatc tcgctttgaa ggccagagtt atggaacagc
    15901 aagggattat gagacagttg cctaccgacg accaaacgat agtcgactat ttgtatcgta
    15961 tcgccggaga atatggggaa aaagccgctg tattgatggg ggacgcggcg ctgagctatc
    16021 acgatcttaa tgcacgctct aaccaactgg cgcattatct gcgtgggctg gggatcggcg
    16081 aggatcgtgt ggtagctatc cgcctgccgc gcggcatggc aatgctgatt gccattttcg
    16141 ctattgtaaa agctggtggt gcctatttac cgctggcgta caatgcaccg cgcagccgca
    16201 ttgaaaatat actgagcaac agcggcgctg tttgtctgat cggtactgac gatggtgatc
    16261 gctggccgat tcctcgcgtc gaaatcgaca gcgcggcggt cagtgccatg ccaacgacgg
    16321 atctgcgtta ccgaccacat gcgcggcaac tggcgtatat tatttacacc tccggttcca
    16381 ccggtgtccc taaaggggtg gcgacggaac atgcagcgct gctaaaccga attgtctgga
    16441 tgcagaacgc ttatcctatc agttctcagg acgtgctgtt tcagaagacg gtgtacacct
    16501 ttgacgtctc tgtctgggag atgttctggt gggcgatgta cggcgcatct gtagtgctgt
    16561 taccgtccgg actggagagc gatccgcgaa ccttggctcg actgattcag cgtcaccgcg
    16621 tgtcggttgt gcactttgtc ccttcgatgc tgaacctgtt tgtcgagtat ctggagatga
    16681 aacaggatcc tcgtttgacc gcctcattgc gattagtgtt ttccagcggt gagaaactca
    16741 cggtccacag tgtggctcgc ttttatcaat cggtggcgca gggtgatctt ataaatctct
    16801 atggcccgac cgaagcagca atcgatgtca gtcatcaccg ctgcctgcgc gggtacgact
    16861 acgacgatat ccctatcggt caagcgattg acggttgccg actctatgtg ctggatgacc
    16921 atggtaatcc ggtagcagac ggcgaagagg gcgaactgta tctcgctggt atcgggctgg
    16981 cacgtggcta tctcaacaac gtggcgttaa ctgatcgctg ttttactata catccaactt
    17041 tgcgccattt agggaaaccg gagcggctgt ataaaactgg agatctggtg tggcgcgacg
    17101 gggaaagcca acaaattcat tacattggcc gtaatgattt tcagattaaa attagagggt
    17161 tgcgcgttga attgggagaa atcgaagccc atgcgatgcg tttcccgggg gtacagcagg
    17221 cagtcgtggt ggcggatcag gatgatcccg acaatcagtt gatttacgct tttgtcgtca
    17281 gtagcgtgcc gctcaatttg gcggccttaa tggacgcact gtccaaaaac ttgcctgcct
    17341 acatgctgcc gaaccgtttg ttggcaatgt cagagttacc actctccgac aatggcaagt
    17401 gctgtcgtaa aacgttgctc gacttggcgc gggcgtactc agccagtcgc gtcgatttac
    17461 gtgaaactcc cgccgtgcgc tacctaccgt tgtcatcggc tcaatcgtcg atgtggttta
    17521 tgcaacaatt ggcgccgcat actgcactat acaataaccc caccgccttg ctgcttgaag
    17581 gagaactgga tcgcacgcgg atggacggcg cgattcgtca attgatgagt cggcatactc
    17641 tgttacgtgc tatggcggaa acccacaatg gacaaccagt attggcggtg cctcagtgcg
    17701 tatcgtcgca ggcgttgctg accatagtgc cactcccctc ggtgagtgat gataacgcgc
    17761 tacaggcgat gatcaaccag cgtgcggcac accccatgcc cttaacgtca ggcacaccgc
    17821 tgtgccggtt tgaactgttg acgctcgatg acgatcgtag cgtattgttg attcatctgc
    17881 accacatcat cagtgatggc tggtcgaaag gcgttttgtt acgcgaattg caggccgcat
    17941 ataacggtga atcgctgacg ccagaaccgt tgttggagta tgccgactac atggagtacc
    18001 aagaagagtg gcgtcagagt gacgcctatc aggacgcgat gcgttactgg caaaataccc
    18061 tggcggggac gttgccgatt cttgatatcc ccaccgatca accgcgtcag aaggtggcac
    18121 gttaccaagg cgcgtttgtt gccttcgcat tgtctgccaa cacatgcgag cgagtgttgg
    18181 cggcggcgcg tgcgcagcgc gtgtcgttgt ataactatct gctgaccgcc ttcgtcctcc
    18241 tgctgcatcg taatgcgcgt caacaggaat acatcgtcgg tatgccgatt gctgcgcggc
    18301 tgacgaaaga acaggagcat atgatcgcgc cgctggtcaa cgtactaccg ctgcgcttac
    18361 ctcttgacga agcggcatcg ttttccgagt tagtacagac gatcagaggt attctgtttg
    18421 ccgctttcag gcaccagcgt ctcgaattta ccgacatagt gcgcgcagtg aatgtggatc
    18481 gcagcgccgg acatttccca atatatcagt gcatgttcca actcgacaat atgcctctgg
    18541 cgagcccgac actaaacggt gtcaacgtta caccattatt gttggatacc agtgcatctc
    18601 aggtggatat ttcgctaagt atgcagcata tcgatgggcg catcaccggc actttcgaat
    18661 acgacgctgg gttatacagt gcggatcgta ttcagcacct ggtggcgcag tggagagaac
    18721 tgctagatga ggccagcagc caacccacgc agttagtccg cgatttgatt cgtttcaccc
    18781 ctcgagagca cgcttggctg gcgcggcaca acgccactga agttgctctg ccgcccgtag
    18841 ataacctgct ggcgctagtg ttaccgcact gccagcagcg ccctacacag gtggctttgc
    18901 gccacgctga cgacgccatg acctatggcg aattgcaaca ggccacgatg cagatgtgta
    18961 cttggctgcg tgcgcaaggc gtaaaacgcg gagaatctgt cgcactgcaa ctgccttttt
    19021 gtttcgaatt gattatcgca caattggcga tcctctcttt gggggcgagc tatgtgccgc
    19081 tggatggtaa cgcgccagcg gcccgcaatg ccctgatctt ggcgcaggca acgccgtgca
    19141 tgctgctggt ggcgcaaccg ctggaatccc ctcatgggct gacgatacct tgggtgctag
    19201 tgcctgactg gcgtagcctg ctgacagaaa taccgaacct gccagttagt gttgcgccag
    19261 atgctttaga ttgcgatgcg gtggtgatct ttacctccgg tactaccggg cagcccaaag
    19321 gcgtccgact cagccaacgt aatctggtca acttaaccgc atcctttata tccagctatc
    19381 aagtgacgca tcaggatgtt ctgctaccca ttacctcagt cgcgtctgct agctttgtcg
    19441 gggaggtact gccgctgctc gccgctggcg gtaccttagt attggcgcaa aaagcgcaga
    19501 gtttggatag tgatgcgctc attgcgctgc tggcatctca gcgggtgact atcctgagca
    19561 ccaccccatc gctttctgcc agcctctctg tgctggctca gtcgatggga tcgctgcgct
    19621 tgtttctgtg cggtggtgaa gcgttagagt atgagcagat agcgccgctt ctgccgcaca
    19681 tggcagtggt taacggttat ggcctgaccg aaagcggtat ctgctcgacc tactttcctg
    19741 tcgcaaagcg tagggagcaa gaaacgggag cattgcccat tggccgcccg attcagaaca
    19801 cccaagctta tgtggtggat gcatataatc gtttggtacc gccaggtgcc tgtggagaac
    19861 tctgtttctc tggtctgggc atttcgcccg gctaccttga tgcacgacag gatcccgagc
    19921 gctttgtcga gttgccggaa taccctggcg ttcgggtgct gaaaactggc gatcgtgctc
    19981 gttgggcaac cgacgggatg ttgttctatc tcggcagaca agatcgccaa gtgcagatcc
    20041 gtggataccg agttgaactg ggcgacatcg aaagcctact gaaacagcat ccggatattg
    20101 ctgatgcgtg ggtcgatgtg cgacgcaatg cggcggcaac gccgctacta gtggccttct
    20161 attgcagcgt caacggcgtt gcgctggatg ctcagcaatt gcgcgtatgg ctcagcctgc
    20221 ggcttccgtt gcatatgctg ccgttgctct acgtgccgct gagcgccatg ccgctaggtg
    20281 taaacggcaa aatcgatccc cagtgcctgc cgctggtcga tctgcggcag ttggaagggc
    20341 cgggcgagta tgtccctccg gcaaccgaac tggaacagcg tctggcggag atctggcagc
    20401 agttgcttgg cctggagcgt gtcggcacca ccacaaattt cttcgatctc ggtggacact
    20461 cacttctgct agtacagatg cagcaataca tcgggcagca gtgcggtcag cacgtggcgt
    20521 tggttgacct gctgcgcttc actaccatca aacgcttggc ggaatttctg ctggccccgg
    20581 acgcagcgca agggaccaca ggagatcaga cacaactgcg tgcggcgaag cagcgtttgg
    20641 cctttggtca cacgcgctgg gcagccacaa ccgacagtca tcactgattc cactgaatga
    20701 aacgcgtgat aaacctgaat atagggggaa agcaacatgg cagagaatga ttttggtata
    20761 gctatcattg ggatggcggg gcgtttccct caagccgata cggtacaggc gttttgggaa
    20821 aatcttctgg caagccgaga gtgcatttcc ttttacagcg atgaggaact gctggcgatg
    20881 ggaatcagtc ctgaatttgt tcagcaccca gattacgtca aggccaaagg ggaggtagct
    20941 gacatcgata aatttgatgc tgctttcttc ggtatcgcac cccgagaggc ggagctgatg
    21001 gatcctcagc accgtgtcct gttggaaacc gcgtgggcag ccttcgaaga tgcgggctat
    21061 gtggctgccg attatccggg ggatgtgggt atttttgctg gcaaaagcat ggattcctat
    21121 ttgatgctga acttgatgcc gcactttaaa cgcgttttct cttctggcag tttgcaggcg
    21181 gccattggta acgacaaaga cagtatcact actaccatcg cctatcacct caacctgcgc
    21241 ggcccggcga tcacggtgca gacttcctca tcgacatcct tggtggcggt gtgcgtggca
    21301 tgccaaagtc tgttgacttg gcagtgtgac atggcgattg ctggcggagt aacgctgggg
    21361 ccaccagcaa aaaccggcta cttgtcgcag gaaggtggga tcaccgccgc cgatggccac
    21421 tgtcgggcgt tttccgataa cagcagcgga tttgtgcccg gcaccggcgc ggggctggtg
    21481 gtgttaaagc gtgttgatga agcgctgcgt gatggcgata acatctatgc ggtcatcaaa
    21541 ggatttgccg tcaacaacga cggttcggag aagatcagtt ataccgcgcc gagcgtggat
    21601 gcccaagcgc gcgccattgc tcaggcacag cggttggcag gtttgacacc gcaggatatc
    21661 acttatgtgg aagcccacgg taccggtacg cgtttgggcg atccggttga gttttctgcg
    21721 ctaagccaag cctttgcggg cgcgtcacaa aagcagtact gtgcgctggg gtcggtaaaa
    21781 accaatatcg gccatttgga tacggcagcg ggcgtcgcag ggttgataaa aaccgcgctg
    21841 gcagtgcagc agggaataat tcccgcaaca ttgcactttg aacggcccaa tgcacagatc
    21901 gatctgacca atagtccgtt ttacatcaac actacctgtc aaccgtggca accggaaagc
    21961 ggcatccgtc gcgcgggcgt cacctcgctt ggcatgggcg gcaccaatgc ccatgttgtg
    22021 ctggaacaag cgccagccgt tgacttgcaa gcgcgagcgc ctgtacctgc ctacagtatt
    22081 ttgccgtttt ctgccaagac cgacagtgcc ttatcctcgg ggctggcgcg ttttgccgat
    22141 tttctgcaac atgaatcgct gccggatagg cgggatctgg cgtggacact ctcacaggga
    22201 cgtaaagcct tcgcacatcg cgccgcgctc gtaaccagag atctacatgc tgccgggacg
    22261 ctgttgcagc aggccgcgac agcgccgttt gctcgtggtg tagcgcagac acagctcggg
    22321 ctggggctgt tgttttccgg gcagggtagc caatatcagc gcatgggcca tcagctctat
    22381 caggtttggc cggcctacgc cgatgccttc gaccgttgcg cgacgttact cgagcgtgaa
    22441 taccaactgg atatccgcca tgagttgttc agagcagaag tctcgttagc ccaaggcgaa
    22501 cgccttgcgc aaacctgcct cacacaaccg ctgctgttca gcgtcgagta tgccttagcc
    22561 caattgtggc tcagttgggg aatcacgcca acggtaatga ttggtcattc gctgggcgaa
    22621 tgggtggcag cgacgttggc aggcgtgttc tctttagagg atgcgttgcg cttggtggcg
    22681 cgtcgagcag agctgatgca tcaggcacca agcggtgcca tgttgatggt ggcgctgccc
    22741 gaagcacaga ttcgcgcact gattaccgcc ccgctggcga ttgccgctgt taacgcccct
    22801 gactattcgg ttatcgccgg gcctacgtcg gagatcctcg ccgtcagcca gcgtctgacg
    22861 gagcagaaca tcatcaacaa acgattgcat acctctcatg ccttccactc tagcatgatg
    22921 caagatgcgg cgcaggcgct acgtcaggca tttgagaatg tacgactgaa cccgccgacg
    22981 ctcaccatca tctctaccgt aaccggcgcg cacgtcagtg ccgacactct caccacaccg
    23041 gactactgga ttgaacagat gctgatgcct gtgcagttct ccgctgcact gcaagaggcg
    23101 caagctacat ttgatgtcga ttttctggaa atcgggccag gtgccaccct gacccagctg
    23161 accaacggtc atgcattagg tgatcgtctc gcgttcagct cgctgccagc aggtgcccgc
    23221 agcagcgatg agcacaaaca tatcttagat accgtggcag cgctttgggt gcgagggcat
    23281 aacatcgatt tgtctgcgtt tgcaggtgag caaccgcgcc gtgtctctct accgacctac
    23341 gcctttgaca aaatccgtta ctgggtcgac agtccagaag aacagaggag cgccgtaacg
    23401 ccggtggcgg acgcgggaag tgtcatccct agcgaaccgt cggtgcgccg tcagccgcgt
    23461 ccggcgtttt cggtgcctta cgcggctcca gagagcaaaa cgcagcgcgg cttagtggcg
    23521 atttgtgaag cattgctggg aattgacggc ttgggcattg acgacaactt cttcgaagct
    23581 ggcggacatt cattgatgct gggcatgttg ctggcgcagg tacaggaacg gttcgccgtc
    23641 actctttcct ttttcgacgt gatggaggat gccagcgtgc gcgcgttagc gcagttggtc
    23701 gagcaggagc agcaggatga tggagggtcc gcacttgccg tgctagttaa cgacatgatt
    23761 aatgagtgac gttggcaaaa gtatttaaca cagcgaaaag gaaaggacgg tcatgacgat
    23821 acatcatgcc gcattggcgc gaatgttacc ggcggaaaaa aaggaaaagc tattacgaca
    23881 gttggcgcaa tcaggcgtgt cgcccagccg cattcccatc atcaaagcgg atccggcgca
    23941 ggcaatcccg ctgtcgttta atcaggagcg gttgtggttt ttgcaaaaat acgacagtac
    24001 cgccactaac tacaaccttt atgtggtgta tcgtttgcat ggcgtggtgg atatgccgat
    24061 gctcacggag gcattgcgcc atgtgcaagc acgccacgcc atcttgcgta cccgtatcat
    24121 agtacgtaac gatcggcctt gccaggtgat agatgatgcg tcttcgttag ttctcgatac
    24181 ggtcacgctg gcagcgcagg ccccaacttc agcgctagat gcggtaattc agcaggtgat
    24241 aaacacccgg ttcgatctgg cacgcggtcc tctgtggggg gtaactcaga ttattcaacc
    24301 cgatcaaggt tgtcatttgg tgttttgcgc gcaccatatc atcatcgacg gtatctcgtt
    24361 acggctattg ttcgatgagt tacagcagca gtatgctcga ttgcatgctg gtaatgaaac
    24421 gtcgctgcct ccgccgccgc tgcaatatgc cgattatgcc ttctggcagc gcgaatggtt
    24481 ccaggatacc ttgctggcga acgaacttgc ctactggcgt gcgcgtttgc aagacgcgcc
    24541 tctgctgtcg acatttccat cgctgcaccc gcgcccggca caaccctcta cacatggttc
    24601 ccggttcagc atcaccctgg atgaaacatt aagcctcgcg ttgaaacacg tagcccgcac
    24661 gcaggaaaca acgccgttcg tgctgatgct gactgccttc caactggtgc tgatgcgtta
    24721 cgctcagcaa cagcgattgg tgataggcat gccagtatcg gggcgcattc gcccggaatt
    24781 gcagagtagc attggttatt atgccagcac tgcggttatc tacaccgatt ttaatggggt
    24841 tgaggtaggg cgcgaggcgc tccagcgtgt gaaggccagc gtgaaagaga cgcaggggcg
    24901 ccagcaactg ccgtttgaaa acttggtgaa tatgctggat ctccctcgca gcctttccca
    24961 ttcaccgttg ttccagatcc tctatatcta ccataaccac gtgaccccac gcgcttttac
    25021 cttggctggt gcgtattggg agcaggtgac gtatcacaat cagaccgtca aatacgacat
    25081 gacagtcgag gtgttccaaa acgacgccac gttcgacgtc tcctttgagt acgatttggg
    25141 gttatacgat gctgatgtgg tgaagcagat tgccgaggcg ctgcgtcagc actgcttatc
    25201 gttgacatca tcactggaga ccccgatagg ggcgatccct ctgcatgcac cggagaccgc
    25261 aacgccgcgg cgtgatccgc tcaacgccac taacgtcccg tggctcgggc cgcaggatgt
    25321 gctgcgtatc attgaacagc gctgtgtgca gcacccaaag caactggcaa tacagcagca
    25381 tgacggcaca ctgacctacg ctgagctctg ggcgcgtgtg cagttcatcg cgatgcgttt
    25441 tcgagcgcat ggcatacaac cgggcgatcg tattggcgtg ctgttgccac gtcacaggga
    25501 tgtgattgca accatgttgg cgacgtggtt tgtgggggcg tgctacgtgc cgttcgatat
    25561 tcatcagcct gccgcgcgtt tgcaacgcct tatgcaacgc gcgcgtttgg tctgtctggt
    25621 ggtccgtcag ccgggagagt ggggtgaaat tgtacaactg tcgttgccgg aattgatgca
    25681 ggacatgtcg aatgcgatcc ggtattctac accttgcgcg ctgttgccgg atatgcaggc
    25741 ctacctgttg tttacctccg gcagtaccgg tgaacctaaa ggcgtgtgcg ttgttcaccg
    25801 cgggttgctg aacttgttgt tggatatgca gcgtaccttt gcggttggct cgcaagaccg
    25861 gctgctctcg gtgacgacgc caacatttga tatctcattc ttggagtatc tgttgccgct
    25921 gatctccggt gcgagtctct atctgacaga ggcggaacgc gccgcagaca gcttccgtat
    25981 gattccgctg attgccgact atcgaccaac gctgatgcag gcgacgccct cgttctggca
    26041 cgggctgttg atggcgggtt ggcgtggcga cccggaacta tgtgtgctgg caggcggtga
    26101 agcgctgcca acgaaagtgg cggaagaact gttgcgctgt tgtggttcat tgtggaacct
    26161 ctacggtccc accgaaacaa ccatttggtc gctgaaatcg cagataaccc aagcggaaaa
    26221 catcaccctc ggcgctccca ttgccaatac ccgtatatac attctggaca atgagggcca
    26281 tccagtgccg caaggcgttg acggcgagct ttacatcgcc ggggatggtg tggcgcaagg
    26341 gtatgatggg cagcctgagt tgaacgcaca gttcttcttg tcagaaccag gggtccccgg
    26401 tggcaggatg ttccgcactg gcgatctggt caggagtgat gcgcaggggc agttgttttt
    26461 tgttgggcgc aaagatagcc agatcaaact gcgcggttat cgtattgagt taggcgaaat
    26521 tgaacggacg ttggcacggc atccgcatgt ggacgccgcg gtggtggcct gtattgaaag
    26581 agcaccgtta cacaaagcac tggcagcatt catcatcacc agtgagcctc cctcgctatt
    26641 cgagcaactg aaaaacgagc ttcggcagca actgccagac tatatggtgc caacgctgtg
    26701 gcagagggtg gccgacttcc cgaacactga caacggtaaa atcgatcgta agcggttagc
    26761 ggaaaatttc gttgccgata gctcccttgt gtcgccgcag acgcaagcgc tgagcgacac
    26821 ggaacagatg ttgctggcgc tgtggatgcg ctatttgccg ataaaaaacg tcgatcctga
    26881 gtgcgatttc tttcgcttgg ggggacattc gctgttggcg gtaacgttgg tagcagagat
    26941 caaccgcact tttcactgcg ccttgaccct gaaggacatt ttccactact ccacactacg
    27001 ggcactgagt gcgcgtattg cacagcaatc tatcacggac gctgccgcgt ctcaggatga
    27061 ctgggtgata gtgcacgacc ctgagcatcg tcatcaaccg ttcccattga cggatgttca
    27121 gcgcgcctac tggcttggac gacagacggg tgctacctcg atcgcgaccc acatctacca
    27181 tgaatttgac gtagaacact ttaatgttac gcgttttacc catgcggtga atgcgctgat
    27241 cgctcgccat gaaatgctac gtgcgcgggt actccccgac ggtactcagc agattctggc
    27301 gcaagtgccg gcgtatcagt tagagcagcg cgatctgagt gctttgtccc ctaacgcacg
    27361 aaacgatgcc ttgatggcga tccgcgatcg gctgtcgcat catgtgcatc ccgcagatcg
    27421 ttggccgctg tttgatttca gttattcggc ttgcacggcg caacatggcc gcttgcattt
    27481 cagtctcgat ctgctgattg ccgatgctct gagtatgcgc acgctacagc aggagttgat
    27541 gatgctgtac cgtgagcccc atgtgtcact gccgttgcta ccgttctctt ttcgtgacta
    27601 cgtgcaggcg ctgttggtag agcaggcgag tgaagcctat gcacgcgatc aggcctattg
    27661 gcaacgggcg ctgccgcagc tgtatggccc accaacgctg cccgtacagg gcgatttggc
    27721 gcaactgtct gcgatcaggt tcgtacgtcg ccgtcatcgg ctgtcagccc acaactgggg
    27781 agtgctgagc gcgctggccc aacgcacacg tatcaccaag acggcattgt tgttgacagt
    27841 ctttagccaa gtgctggcac gttggagcct tagcccgacg tttacgctca atctgacgtt
    27901 gttcaaccgc ccgcagggtt accccaacgc agaggcagtt attggtgatt ttaccgctgt
    27961 cagcttgctg aatgtttgtt acgacagcca gcactcttat gcccacaacg ctcagcgtat
    28021 tcaggtgcaa ctgtgggaag atctcgaaca tcgtcgtttc agtgggatcc gcgccagcga
    28081 ggcgctgatc catagcggtc gtttccatgc gccgatgccg gtggtattca ctagtatgtt
    28141 ggatatcgac ggggagacga ctgcgcaaga ccctcgggac acaacccgtt ttactctgtg
    28201 tccggacgcc aatattaccc aaacaccgca ggtgtggctc gatcaccagg tgatcgagtt
    28261 ggctggggag ttgcatttca actgggacgc ggtcgagcaa ctgtttgata ccacgctgct
    28321 ggatcagatg tttggtgctt attgtcatgc gctgcaggcg ctggttgcca tgccgcaaag
    28381 ttggtggggg gtaaatagtt ctctggcgct gcccaccgtt agtgcaccgg tcacgcaggc
    28441 tcctgcacct acggccttgt tgcaccatgg attactgcgt caggcagcac tgacgccaca
    28501 ggaaactgcg ctgatcactc ctatccgtga attgacctat cgccaactgt cgacggcggc
    28561 ggatcatgtg gcccgcgccc tgttagcgct gggcgtgcag catggcgacc gcgtggcggt
    28621 ggtgatggaa aaaggctggc agcagattgc cgccgtacac ggcattttac gactgggtgc
    28681 ggtctatctg ccagtggatc cggtgctacc gccacagcgt cgccagcttt tgctgacggt
    28741 gggcgaggtg cgggtacaag taacgcagcc gggtctcacg caattggagc cgtcgctgcc
    28801 cgtgctgatc atcgacgacg gaatgctgga cacgcctgct gcgccgttgc ctgaagtggc
    28861 tggggatgtc acggatctgg cctatatcat tttcacttcc ggctccaccg gtaccccgaa
    28921 aggagtgatg atcgaccacc gtgcggccat gaacacgctg gaagacatca acgaacgctt
    28981 tggcctcaat gcgcaggata gggtgttcgg gctgtcatca ttgagctttg acctgtcggt
    29041 ttacgatgcc tttgcgcctt ttatggtggg tgcagcgctg gtactgccgg aagcaggacg
    29101 ggaaaaagat ccgcgtcatt ggcagacagt tatggcacac ggtcatgtaa gcgtctggaa
    29161 tgcagtgccc gcactgatgc agatgctgtg cgaataccac agcggcgatc ggatgagtta
    29221 tccgacgttg cgtctggcac tgttgagcgg cgactggatc ccgctaacgt taccggagca
    29281 gatgcgcgag cggctcaatg aaacgatgga catcatcagt ctgggtggag cgaccgagtg
    29341 cgccatctgg tcggtctact acccgatagg tgaggtggaa tcgacgtgga ccagtattcc
    29401 ctacggtcgg ggcctgcgca accagccagt atacgtgcta aatgcgcaac tggaggaatg
    29461 tccggtcggg gtggaaggag agatttgcat tggcgggatg gggctggcac aaggctacct
    29521 gaacgacgca gagaaaacgg cggcgagctt tgtctggcgc gaagcgagtg gtgagcgaat
    29581 ttaccgcact ggggatcgcg ggcgctactt tgctgacggg caagtcgcct ttttggggcg
    29641 caacgatacc caagtgaagg tgaatggtta ccgtatcgaa ctgggggaaa tcgagcgctg
    29701 cattgcgcga catcccgatg tggagcagtc agtggtggtg gcagtgggta attctcaaca
    29761 tcgtcggctg gtcgcttttg ccaaactgca cgatcgccac caggcgcagg cattgcaagc
    29821 taaggaagcg gaggcggcgg cactggcgca gggtattatt gtgaatccgg cacagcgtct
    29881 agcgttcaaa ctcaaggagc cacatattcg cgcgctggat ggtctgggca ttgcactgac
    29941 ggcaccggcg gatagcacac gttacatcaa acgccgcagc tatcgtcatt tcagcgcgca
    30001 aaaaaccacg ctggcacagt tggggcaatt gctgtcgggc ttggggcaga tgcgtctacc
    30061 cggtctacct tttgccaagt atgcctatgc gtccgccggg gggctatacc cggtgcaaac
    30121 ctacgtgtac ctgcatccag acaagatcga agagggagta tccggtattt actacttcga
    30181 cccgcgacag agctgtctta tgccggtagc accagaagtc gagctgaaca gtggttttca
    30241 tgccggacct aatcagtcta ttgccgatcg ggcggcattc acgctgttta tggtggctga
    30301 tatggcggtg atctcgccat tctatgggca agaggcagct tggcacttct cggtgatgga
    30361 agcaggtact ctctgccatt tactggaaga agatgcgccg cgctacggat tggggctgtg
    30421 ccaacttggg atggcagact tttccgctgt ggcatcgcat tttcaattgt cgccacatca
    30481 tcgctatgtc cattgcaccg tggggggcgc gatagggcaa gaggcggcaa gtgctgcagc
    30541 attgctgcgc gatttctcca cctatgagaa accgaaggaa accgctgcgc cgctggacat
    30601 gcagagctac aaagatgcca tgctgcgcgg cctgcgtcag caactgcctg actatatggt
    30661 gccgagtgat ctgatgttag cgaccgattt tccgttaacc gctaacggca agctagatcg
    30721 gcaaaaatta cagctgcagg gcgaacaaat tgcccaccag cgtgacggcg tgggtccaat
    30781 ccaggtggac agtgcgttac aacagcggct ggtggcgctc tggcaggagg tactcggcgt
    30841 gagccacgtg tcggccgaag acgatttctt ctcgctgggg ggcagttcta tagaattggt
    30901 gcgtattcag caggcacttg aggcgattat cgggcaggag attcccattg tcgatctgtt
    30961 ccgtctgcca accatcgcgg atgtggcgcg ttaccttgat gagcaactgc acaatctacc
    31021 cgccgcccac gatatcgtat tagcgcaggc tgaggtttcg caggtcagcg ctgcgcgcga
    31081 gaatctggcg ttgcggcgta aacgtgccca acagggagaa aagggcgatg agtgagcaga
    31141 gctatcgtag cgcagggaca ctgttggcac agttggcgtc cggagaaacc acctcagtgg
    31201 cgttggtgaa tcactatttt tcacgtatgg cgcagtttaa caagccgttg aatgcggtgg
    31261 tgcagcagca ctatgcgttg gcgttggagg ccgccgcgcg cgccgatcgt gagcgactgg
    31321 aggggcgcgc aaggggcgtg ttgcacggat taccttgtac tgttaaggag tcgtttgacg
    31381 tgcaagggtg gttaaccacg tccggtgccc attatctgaa agataaccgg gcgacgcagg
    31441 atgctccttc catcgcacgc ttacgcgctg ccggggcgat cctgatggga aaaaccaatg
    31501 tgccgatgat gactgccgat tggcaaacct ataacgatct gtatggcacc acccataacc
    31561 tgtgggatag gcaacgttca cccggcggat cgtccggagg agcggcggtg gcggtggcgg
    31621 cggatttcac ccccgtggaa tttggcagcg atttgtttgg ctcactgcgc attcccgcac
    31681 actacacagg tgtctatgcc catcgttgta gcttggggtt gatgtcggtc agaggacatg
    31741 tgcccggtgg tggtccgcaa gcgactgatg agccggatct ctcgacggcg ggccccatgg
    31801 cgcgcagcgc ggcggatctg cgtctgatga tgcgcgcatt gagcacattc tgggtggaac
    31861 cgccgcgtat tcccgatttt agccgttatc aggccaaagc aaactaccgc gtgtgcacgt
    31921 ggtttagtgc gccccaccat gagatagatc aacagatcgc acagcgtttt caatcgttta
    31981 tcgacaagtt acgcgcacag cctggcgtgg aggttgatga tgctatgccc gccgatatcg
    32041 atcccgacgc actgtttgat atagcagtca aactcagtgg tcggctggta agtacggcgc
    32101 tcaatggacg ccagcgtctt acagccgggc tggctgcgct gggcttcagg cttgtgggca
    32161 agctggcgga tgtgcctgag gggataacga gctattacca gggcatgctg aaagacagtg
    32221 gcgaacagcg gaatacagac aaactccgcc acgaatacag ccgggtgata gaaacccttt
    32281 tcgcgcgtta tgacgtgttg ttgacacctg tcagcccggt gctggcgttt gcgcacatgc
    32341 agcagccggt gcgtaagcgc aagcttatcg tcaatggcga accgcaagat tacaacgaac
    32401 atttgttctg gaacatgtta gcaacggttt ttggtttgcc tgccaccgtt tacccattgg
    32461 cgaaaacgat ggatgagctt ccgtgcggca tacagatcat ttccgggcat tttcacgatg
    32521 atgtgacgat taactttgct gagttctgcg aaagcatcag tggcggattc acggtaccgg
    32581 aagggtacta gaggcggata tcggtgacag cgccaataaa ggtgcggcgc atttaatgaa
    32641 taaggagcag gtatgacggt gaataagctt gagccagaca acggcacgcc gaacgatgag
    32701 ctgttcacgg tggcgggtat gtttgacggc tcgctgtata aattactcct gcgcatggcg
    32761 ttgccgatgt ttgtcggtat gttaacgcag gtgacctatg ccatcgccga tattttttgg
    32821 ctgagccaca ttgatgtcac caacagcggc atcattgccg gtgtggggct ggttttcccc
    32881 gttggaatgg ggttatttgc catcgccaac ggcattcaaa tcggtatggg atctttgctg
    32941 tcccgcgcca tcggcatgca gcggttggat cgtgcacagc ggattttgtc ggtcggtatc
    33001 atcatcgcgc tattctttgc gatcgtgatc accgtacttg gctatgttta tgcccagccg
    33061 ctattgcgct cactgggggc cacgaagagc atcatcggct acgcaacgga gttctattat
    33121 tattcgctgc tgaccgtgtt cagcatcatg ttgattggcg tcatgatggg gctgtttcaa
    33181 ggcgcgggta agatcatggt catcatgaag gcctcattgt tgggggcgtt ggtaaatatt
    33241 atgctcgatc caatcatgat ctttgtattc gattttggtg tgaaaggggt ggcattggcg
    33301 tcgtttctgg cgcaactgag tatggtcgcc tatttcatct acacattgat gggattacat
    33361 atcgggttga gtatacgcat cgcgttacgc ccattctcct ggaaaatcta ccgagagttt
    33421 ctctccgtcg gcatggcgca gatgctgatg caactgatca ttgcggtcgg catagtgatt
    33481 tataattttt ttatcgtcag gctggacgta aacgcgatgg cagcgtttac gctcactggt
    33541 cgcatcgact atttcatcat cacgccgatg ctggctatcg cgaccgcgtt gctgactgtg
    33601 gtggggcaaa actggggaca cggaaatgtc acccgcacgc tgaatgccta ttgggccgcc
    33661 gtcgcgctgg ccttctctat tgtgctggtg ttagccgtta tgcatattgt actggcaccc
    33721 tggatgtatc ccctgtttac tcgtgttgtt gccgtgagtg actatgcggt actgcaaacc
    33781 cgtatcatgg cactggcact accctttgtc gctatcagcc tgttagcaag tgaatattat
    33841 caggccattg gtaagccttg gtattcggtg ttgttgacgc tgatgcgtca cgtgtttata
    33901 tctgtccctg tcgtatacct gctggcgatt gtgttagaga tgcgtattac cggcgtctat
    33961 tttggggcga tgagcggcac ttttgtggct gcgttgttgg cgtggcgtct gctgcgtctt
    34021 tcacctcgtc tgctgcgatg gaatcaggag gcggtgcgct cccaacactt ggatatggag
    34081 gtggcaccat gatgtcgggc aatccgttgt cgtggccaca ggaacagtgc cacatcattg
    34141 atcaactgta tccttacagt gccgtaaaca tcattggtgg tgtggtcacc attgagggaa
    34201 ttgtcgatct gccccgacta cacgcggcca tccagtccgc cattcggcaa tttgatgcgt
    34261 tgcgtatgtg gtttgtgatg ggagaggaga gtgaagtggt cagccaagtg cagccttatc
    34321 actggcgcga tatccgccat ttgacttttt ccccagatta tgacaaggag aacctacgcc
    34381 cagcggcgat cgaaacattt gttgatgagt ggttccggca gccctttacc ctgctggcgc
    34441 acgatttgtt cgagtttgtc acctttacgt gcggtgaaca atacagcggt tatttattta
    34501 aagcccatca cggcattgct gatggttggt caatggcgtt gttgtcaaac catgtcaagc
    34561 gagcatacga acagcaggac gtaccagatg atgcgtcacc agcctatagc gcatttctgg
    34621 cgcagcagca atcttatcag gcgtcaacgc gctttgccgt tgatcgtggc tggtggcgtg
    34681 actatatcga cgaataccgc gactgctttc cagacagttc ccccatcgtt actactgagg
    34741 gcatcagttg ttccacttgg cttgagccag ccatgattaa ccgtctctac aggttgtgta
    34801 atcgttatgg ctgtacgctt aatacgctgt ttattgcact cttcgctctc tatcgcgcca
    34861 gagtatgggg ggaggaaaaa ggcgtaatag gcgtgccgtt agccaatcgg catactcgcg
    34921 aggcgcggcg ttgctttggc atgtttacta atcaattgcc gctggcctat cgactggtgc
    34981 gcacggaacg attctgtgaa cgggtggcct ttttccagcg cgaactgaaa cgcggtttta
    35041 aacacagcaa atatcccatt accttattca accaggattt ggcggaacag gggggaggga
    35101 agttgcgggc gttcgattat tgcgttaatt attataactt tacctatgaa cgccacattg
    35161 caggtgccgc gcaacgcgtg gagagttact acagcggcga gcagtcctat aagctacaga
    35221 ttgttttgca aactgttaat aatcataaag aaagccttag gttgagtctg gaggcgctgc
    35281 gtagcgcttt cactccgcac caactgacag cgatgaaaaa tgggctgttg gatttagtca
    35341 ctgcattgga tcggcaacct gacgctcgtc taggcgatct cgaagtctat cctgcgccgc
    35401 acgtcgcatt ggcatgtggc tcactcaaac cctcatttac ttcccgattt gcagcgcaag
    35461 ttgtggagca tggtgatcgc acagcactaa ttgataatga acagtctttg acttatcggc
    35521 agttggatga cgctgttgag cgggttgcgc gttatctgcg ccaacagggg atcggacgcg
    35581 gtcaagtggt tgggattatc gctgagcata gcgctcaaac ggtgatggtt atttatggta
    35641 ttttgcgctg cggcgcggca tttctgccgc taaatcccgc gttgccaacg acccgccttt
    35701 acgccatgtg tcgtaaagcg caggtagcgc atatccttta cgatccggcg atgcatgaat
    35761 tgacgcaggc gctggcgttt cccgcgtcca gcttgctcca ggccttagcc acttcggcgc
    35821 ttgctagaga accttggcca gctattgaac cgcaggatct ggcatatgtg ctgtttacct
    35881 ccggatcgac cggcgagcct aagggcgtgc aagtctctca tggcaatctg gctaactatc
    35941 tccatttcgc cgccgaacgt tactttacag cgcaggaccg ggctgcgctc tactcttcgc
    36001 tgtccttcga tttgaccatc actacgctgt tcgccccctt atgtgtgggc gctagcatca
    36061 gcgtttgtcg gcacgccgag agcgaaacat tgctgcgcat ggccgtggtt gatcagccca
    36121 acacagtgat caaactgacg cctgcacacc tgcggttgtt gtgtgcggct ggcataagca
    36181 gtgagcagat tcgtaccttg gtggttggcg gggaggattt caagcgggat ctggcgcgta
    36241 aggcggctgc tctctttcct caggccgtga tctataacga atatgggccg acagaggcca
    36301 ccgttggttg catgatctat cgctacaccg ggcaagaaac gctgccttca ttgcccatcg
    36361 gtatggcgat cgacggttgc caggtagcaa tttgctcccc ttggggctgt ccggtaccgg
    36421 aaggcgaaac tggtgaattg gtgatttatg gcgcgtcagt gacgcagggc tatatcgatg
    36481 cgccgcaaca aacggccgcc gcctacctta aagataccaa tggggtgatg attggctatc
    36541 gcagcggtga tattggctac gccatcgctc ctaatacgct ggtttatcag ggacgtaaag
    36601 acgatcaggt caaaattaat ggctatcgta ttgagctgtg cgaaatcgaa caagcgttgt
    36661 tgagcgcacc tcaggtagaa agtgcggcgg tggcggtgat tgatgatgtg caggggcagc
    36721 acagcgggtt gctagcctgc gtgacaccgt catctgttga tgtagctacc gtaatgcaac
    36781 atctgcgtca gcaattaccc acctacatgc aacccaagca gtgctgtgct atcgcccaac
    36841 tgccgctgtc gcacaatggc aaggtggacg tgcgtcagat ggtcgcaacg gtccgaaaca
    36901 ccgtgcctgc atcgggtagc gagcggctgg gagatgcggc gataaggcat tcagttcggg
    36961 tatgtgtgga aggggcgctg gagcaaactg agtttgatga taacgaaaat ctttatgtct
    37021 tggggttgga ctccatcaag agtatccaga ttgcagcgca gctcaggcac cacggctgga
    37081 cgatgtcagc ggtgcaggtg atggagtgtg gaacagtgaa cgccatctgt gagtttctcg
    37141 ctagtcatac aacggtgtca cagctcgcgc agtatgctca taacacccgt atcgatctac
    37201 ccgcattgcg ttggtttacg cagttggcgt tgccagtgcc caatgtttat aaccatgtca
    37261 ttgtgctgaa agtgttgccg ggctgcccgc tcgagcagtt acacaacagg ctgcatacat
    37321 taatccagca gcagccagct ttgcacagtg cgctggacgc tgaggggcgg ttgctagtat
    37381 gcgaccctaa tgtgtgttat cccaacgagg tgctcacgga atattcgacg gcgcagtgga
    37441 cgttggcaga ggtgatcgct cagtgcaata gcatgcttga cgtgacaaat ggtcgagtct
    37501 ttacggcagc tctgttacac gccccgcagc ctgcgtctag cacgctggta ctctgtgccc
    37561 atcacttgtg cgtcgatatg cactcttggt atttaatcct cagcacgctg gacgccgtca
    37621 gcactgtcaa tggcaccagc aacagcggac tgcatcgctg gaatgactat ctggcgagca
    37681 aaacggtaga ttccgccaca cacgaaagct ggcgcacagt atgtcaaacg ctaccgctgc
    37741 atttcccacc cgtgtcattg ccagatgact cattacctcg tacgcgcgct tggcgtgaag
    37801 actttcgcca tccttgcgtc aggaggttgt ttgaatccag tggtaatacc gcgtacagcg
    37861 ccgaaaccta tgtgctaacg gcattggcgc tggtgctgcg ttactacagc gaagagcctt
    37921 ggtgtcgcat cgagatggaa ggcatgggac gcggctgctg gcccgatgag cctgacgttg
    37981 cggatacggt cgggtggttt actctatttt atccttgggc gatccctctg catggcgata
    38041 tggcgacact gttatctgcg atcgcttccg atttggcaaa acgcacgcat ggtgggggag
    38101 attacggtct gctgcaaatg cggcatgcac cggaagactc tcttgcgcaa gggatccgaa
    38161 tgaattacat cggtgtgcag gcgcaacctt cactccgcta ttttcatatt gatcacttta
    38221 atagtgatat ctataccgcg cctgaaaacg cgctcggctg cgtgctggag tttaatattg
    38281 cgcgctcggc agctgatggt ctctccttcc actgccgttt cgatcccacg cgcattgcgc
    38341 tgaatgatgt gcaactcctg ctggcgcgct acaagaacag cctcactgat ctggatgcct
    38401 ggctgtgcca acacagcgcc acgctgacgg gcgcgccgac tttgtggaca ctataaacac
    38461 gatgccaacc cttaaggaat acaacaatgg caaaggatga ttttacctgt ggctcactgg
    38521 atattgccat tattggcatg agcgggcgtt tttccggtgc ggaatcggtg cccgagtggt
    38581 gggataagtt gctggcgggt gaggagttca ctcaaccgac atgcacagaa gacgataatg
    38641 ggaacccttg gattaggctg cgcaatataa tcaccgctcc ctatgacttc gatgctgcat
    38701 ttttcaatat tccgcctggc gaagccctgt tgatggatcc gcaacaacga atatttcttg
    38761 aatgctgcta caacgctctt gagcatgcgg gctatattcc cacgcaactt aagcgagtcg
    38821 gcgtctatgg ggcgacctac gccaataact attttatcga tcgcgtctat ccatacctga
    38881 agatgagcgg cgatcaccat tatcttcagg cgcagattgg taacgaaaag gattacttgt
    38941 gtgctcaggt tgcctacaag ttggggttta ccggacccgc tgtgagtgta cagaccgcct
    39001 gttccagttc gttggtggca gcatatttag catgcgaggg gttattgact tttcaagccg
    39061 atgttgcgtt ggcaggtggg gtcaccttgg gttttttaca ggcgcacggc tacagtccgc
    39121 aaggtgataa gctggtatcg caagacggac actgcgcccc gtttagcgct gaggcgaccg
    39181 gcacggtata cagcagcggt gcgggagttg tggtgctaaa acgtcttgag gatgcactac
    39241 gcgaccaaga cagagtgtat gcggtgatta aaggaggtgc ggtaaataac gacggcgggc
    39301 gtcggctggg gtttgtcgcc cccagcgtag aaggacaggt agaagcgatc aatacggcgc
    39361 ttgccgccgc agaagtcgta ccgactgata tcgcactgat cgaaacgcac ggtaccggta
    39421 ccccgttggg tgatgagatt gagcttgaag ctctgcaccg ggttttcgca ccggcatgtg
    39481 cgccgcacag cattcaattg ggtgcggtaa aagccaactt gggtcacctg ggagtcgctt
    39541 ccggtatcgt cagcttaatg aaaaccgcac tgacgctata tactggttta gttcccccgc
    39601 agatcaacct agtaaataaa cataaaaaac tgttgcagcc agcctcgcca ttttatctca
    39661 gtgatgtagt gacttctgtc ccacaaacga aaaggatcca tgccaccgtt agttcgtttg
    39721 ggctgggagg gaccaatgct catttggttc tgcaaaactg gtgcgagacg cctgcgcaag
    39781 cggtgcaaga aaatgagcgc cgcctgtttt tcttcagcgc caaaacgcca ttagccctta
    39841 gacaacagtt ggatgctcat tatcacgctc tggcgaccta tgcggaagca gataaagatc
    39901 gtatcgccta tactctcgcg caacgccggg cacatttccc gtatcgctgt gcgctggcgg
    39961 cagacagtgt ggtagcgttg cgtgcaagtt tggcgaaact gcgggatgct gacatgtctt
    40021 ttacccctat caatatggaa actaccttgg tgttcctata tccggaccgg gacgataagc
    40081 tggagagtgc cctgacgcat ttgctggctt gtcaacctaa cttgcgccag cggcaccagc
    40141 gcctgtctca ggatgttgca caaatctgcg aaccagccga ttggacgcca gcattgcgtc
    40201 agtttatcca gcaggtgagt cttagtgaat ggctgatcga acaaagtatc tcgcctgtgc
    40261 agcacatcgg ttacctgacg ggggctgcag cagcgcagta tgttgcgcgc atcatctcac
    40321 tggaaaacgc tgttcagcag gtgattgtgg ctgaaacgac accggagcag acgctggccg
    40381 gcaacagtga actgagcgaa atattggcta atctggcggt gacggaggga acgctgatgc
    40441 tggaaatcgg tcgcgcgggg acgttttcca tattgtatca ccagcatgcg cagtgggttg
    40501 ggcaaacagt attttccccc atgctgaata cggatacgcc tgaggacatc ctgccgctgt
    40561 tgggcactct ttggcaacga ggagtgacta tttgtttacc ggaaatgcca gccgtgcaga
    40621 ctataggctt acccggttat tcgtttgatc gcgtccggta tgaaattcag tccagtgatg
    40681 cgagagagaa cgctatgtta ccagtgagtt atttgtcggt cagcgacttt gtggagaaaa
    40741 cctggcgttc attgttatgc atcgatcatt atgacgaaca cgcggttatt ttcgaatacg
    40801 gagcgacctc gatgcacgtt atctctttcg tcgacagctg taaccacatt tataaaatcg
    40861 gactgactgc cgccgatatt tatgccagac ccgctatccg tgagcacagt gaatttatct
    40921 ccgaatgtgt tgatggtatt ttatgaggtg ttcacaggat gacaataatg gaacacgtta
    40981 gcattaaaac attatatcat ctcctgtgct gtatgctgct ctttatttcc gctatgtgcg
    41041 ctttggcgca agaacatgag cctatcgggg cgcaagatga gcgcctgtcg acattaattc
    41101 accaacggat gcaggaggcc aaggtcccag ccctttccgt aagtgtgacc attaaggggg
    41161 tacgtcagcg atttgtctac ggtgttgccg atgtggctag tcagaaagcg aatactctag
    41221 acacagttta cgagctggga tcgatgagta aggcgtttac cggacttgtg gtgcaaatac
    41281 tgattcagga aggcagactc cggcaagggg atgatatcat tacctatctg ccggaaatgc
    41341 gcttgaatta tcagggaaaa cctgcttccc tgaccgtggc tgatttcctt tatcatacat
    41401 caggattgcc tttttcaaca ctggctcggc tggaaaaccc tatgcctggg agcgctgtgg
    41461 cacagcaact gcgcaacgag aatctgctgt ttgcgccggg tgcgaagttt agctatgcct
    41521 ccgccaatta tgatgtgttg ggcgcggtga ttgaaaatgt gacgggaaaa acctttacag
    41581 aggtcattgc ggaacgactc acgcagccgc tgggcatgtc ggcgactgtg gcagttaagg
    41641 gggatgagat tattgtcaac aaggcaagcg gctataaact gggattcggc aaacccgttc
    41701 tgtttcatgc gcctctggcc cggaaccatg ttcctgccgc ctatatccat agcactctgc
    41761 ctgatatgga aatatggata gacgcctggt tgcacagaaa ggctttgccg gcaacgctgc
    41821 gtgaggcgat gagtaacagt tggcgtggta atagtgatgt tccgcttgcc gcagacaatc
    41881 gtatcctcta tgccagcggt tggtttatcg accagaatca aggcccttac atcagtcacg
    41941 gtgggcagaa tccaaacttt tcttcttgca ttgcgttgcg accggatcag cagattggca
    42001 ttgttgcgct ggcaaatatg aattcgaatc tgatactaca gctttgcgcg gatatcgata
    42061 attatctgcg cattggcaaa tatgctgacg gcgctggtga tgcaattaca gccaccgata
    42121 cccttttcgt ctacctcacg ttgttgctgt gtttttgggg ggcggtggtt gtagtgcgcg
    42181 gtgctttccg tgtttatcgc gcaacggcgc atggccctgg aaaacagcag aggttacgtt
    42241 tacgcgtacg tgactatatc atcgccttgg cggttcctgg gctcgtggcc gccatgctct
    42301 atgtcgcacc gggtatacta tctccaggac ttgactggcg ttttatcttg gtatggggtc
    42361 catcgagcgt gttggcgata ccgttcggaa ttatcctgtt agctttcgtt ctgacattaa
    42421 atcatcaaat taaacgaatt ctattacaca acaaggagtg ggacgatgag taatatcagt
    42481 ttgtattgtt tgccatattc aggtggttct gccgccatgt attataaatg gcgtagcgtg
    42541 ctgtcggaca atattacttt gcggccttta gaacctgcgg ggaggggaac tagaatacgc
    42601 cagccgctgt gtcttacgat ggtggatgcc gtcgctgacc tttatcaaca atttgtgaaa
    42661 cactacacag gtggagacta cgccattttt gggcatagtc tcggagggat catggccttc
    42721 gaactggtgc attatattct cgatcatgga catgacatgc catgcgcgct gtttttttcc
    42781 ggctgtcgcc cacccgatcg ggcctctcat gaagtaatac tgcatacctt gcccgatcag
    42841 gcgtttatgg aagagatcgt caagctgggc ggaactccgg ttgatgtctt tcgtaataaa
    42901 gagttaatga caattttcac ccccatcatt aaaaacgatt atcggctcta tgagcagtat
    42961 gtatttcagg ccaaggcgcg cacattaacc tgtccgatcg tgctatttca tggcgatgct
    43021 gacaatctgg taatgcagga tgaattactt gcatgggaaa aattcaccac acgaaagacg
    43081 cggactatta tatttcctgc tgccgatcat ttttttgtcg ataagcattt tgaacaggtg
    43141 gtaggttatg tgaaccagac gattgaatca ctcgaaatag tagggtagaa atagttgtgt
    43201 aactatacaa ggagcaatag atatggctgt tccatcatca aaagaagagt taattaaagc
    43261 tattaatagt aatttttctt tattaaataa gaagctagaa tctattacgc cccaactcgc
    43321 ctttgaacct ctattggaag ggcacgcgaa ggggactacg attagcgtag cgaatctggt
    43381 ttcctatctg attggctggg gagagctggt gttacactgg catgaccaag aggcaaaagg
    43441 aaaaactatt atttttcctg aggaaggatt taaatggaat gaattggggc gtttagcaca
    43501 gaaattctac cgtgactatg aggatattac agagtacgaa gttttattgg cacggttaaa
    43561 ggaaaataag cagcaactcg tggctttgat tgaacgattc agtaacgacg agctttacgg
    43621 taaaccttgg tataataaat ggacccgagg tcgtatgatt caatttaata ccgcctcgcc
    43681 ttataaaaat gcttcgggga ggttaaataa actgcagaaa tgtcttgcag aatagcgggg
    43741 tgcttaccgt ttgcctcccc actgatgatt tttccgggca gcgatgagtg tggactggcg
    43801 gcactatcac ccgctgctcc ggaacatatt ctggttaatg atacggataa aatgtgtaag
    43861 acaataagtc tacttaacat aagcacatgc gaacgcatgt gtattaagaa ccatgatgtc
    43921 ctggacgact tagaagatga aaaacggcct gtggatgaaa agccgtatca gcgtttgctg
    43981 atacggcctg gttctttaca agattattca taagtcaggt tactgcgaga ttaatgtttt
    44041 gctgactcca gatgtacggg ggcacccaga tttcactgca catatccaga taatccgacc
    44101 accactgcat catgtccatg cgggcttcca tatgttcggc cttatgaatg taagccagac
    44161 gcacagtgtt gcgctcttgg tgactcatct gacgctccac tgcatcttgc gaccataaac
    44221 cggattccat cagcgcgcta cacgccattg cccggaaacc atgactgcag atatcttgtt
    44281 tcgtgttgta tcccatctgg cgaagtgcct tattgatagt attttcactc atcggcttgt
    44341 aggggtcatg atagccgggg aacaccagtt catacccgcc agatatttcc ttaatccttt
    44401 tcagaataga gataacctgc tcagaaaggg gcacaatatg cggtgtacgc attttggcac
    44461 ctcgactgga ataacgtacg cctgcaatcg cttcgcgtgt ggcaggtatc gtccagatcc
    44521 gatttctgaa gttaatctct gtccaacgag catagcgcag ttcacttgag cgaatgaaca
    44581 catgcaacgt cagcaggact gcaagcctgg tgagttcccg tccttggtga taactgtcaa
    44641 tccgttcaag cagctcagga aggcgctcca gtggcagggt gggataatgt cgtctggcgg
    44701 gagaagccgt cacgccgtca agatttgcag ctgggttgat ttcgattaac ccctgatgaa
    44761 cagcataacg cataatgttg ctgaggtgtt gccgcgagcg ggacgcaact tcaagcaagc
    44821 ccttttcctc aatgcctttc agtaattcaa tgaaatgacg tgatttaagc tcggtaactg
    44881 gtagatgccc gatggtcgga aagacatgtc tgttcagacg ggcaagcacg cgatctgcgg
    44941 tattctgcga ccattttttc ttgctactgt gccactccag tgccacggat ttaaacattt
    45001 tctcctgcat gcgcgagccg cgttcagcgg cacgttgctg cgccgggttg atgttcagcg
    45061 ccagcatctt acggatgcct tcgcgctgct ggcgggcgtc agacagggag acggcagggt
    45121 aggcacccag tgcaatacgg gattctttgc cattaatacg gtatttaaga taccagagac
    45181 gggaaccgcc tggtttgacc agcagataca gaccgtggga atcggagact ttaaagggtt
    45241 tatcagtggg cttgagacta cggatttttg cgtcgttaag ggacatatgg gggtcactcc
    45301 attatcgaac taacctgacc ccagatttga ccaccaattt ttcccgatgc agagggtaaa
    45361 atcaaaatgc accgggaaga gttttcacgc taacctgttg aataaaaata acataaggat
    45421 tcgcaaggat gcatgaaagc ag
//
LOCUS       DAZFRG010000005        44912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFRG010000005 DAZFRG010000000
VERSION     DAZFRG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.005"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(329..871)
                     /gene="ycfP"
                     /locus_tag="ACHKUC_000172"
     CDS             complement(329..871)
                     /gene="ycfP"
                     /locus_tag="ACHKUC_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO6616505.1"
                     /translation="MIIYLHGFDSNCPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(894..1919)
                     /gene="nagZ"
                     /locus_tag="ACHKUC_000173"
     CDS             complement(894..1919)
                     /gene="nagZ"
                     /locus_tag="ACHKUC_000173"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO6616506.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1930..2754)
                     /gene="thiK"
                     /locus_tag="ACHKUC_000174"
     CDS             complement(1930..2754)
                     /gene="thiK"
                     /locus_tag="ACHKUC_000174"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO6616507.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENIDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2735..3376)
                     /gene="lpoB"
                     /locus_tag="ACHKUC_000175"
     CDS             complement(2735..3376)
                     /gene="lpoB"
                     /locus_tag="ACHKUC_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO6616508.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3390..3767)
                     /locus_tag="ACHKUC_000176"
     CDS             complement(3390..3767)
                     /locus_tag="ACHKUC_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO6616509.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3770..4129)
                     /gene="hinT"
                     /locus_tag="ACHKUC_000177"
     CDS             complement(3770..4129)
                     /gene="hinT"
                     /locus_tag="ACHKUC_000177"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO6616510.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLTHKGL"
     gene            4464..6653
                     /gene="fhuE"
                     /locus_tag="ACHKUC_000178"
     CDS             4464..6653
                     /gene="fhuE"
                     /locus_tag="ACHKUC_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO6616511.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQNDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTFKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6713..8146)
                     /gene="ptsG"
                     /locus_tag="ACHKUC_000179"
     CDS             complement(6713..8146)
                     /gene="ptsG"
                     /locus_tag="ACHKUC_000179"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO6616512.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8441..9238)
                     /locus_tag="ACHKUC_000180"
     CDS             complement(8441..9238)
                     /locus_tag="ACHKUC_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6616513.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9249..10253)
                     /gene="holB"
                     /locus_tag="ACHKUC_000181"
     CDS             complement(9249..10253)
                     /gene="holB"
                     /locus_tag="ACHKUC_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO6616514.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVIWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10250..10891)
                     /gene="tmk"
                     /locus_tag="ACHKUC_000182"
     CDS             complement(10250..10891)
                     /gene="tmk"
                     /locus_tag="ACHKUC_000182"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6616515.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(10881..11903)
                     /gene="yceG"
                     /locus_tag="ACHKUC_000183"
     CDS             complement(10881..11903)
                     /gene="yceG"
                     /locus_tag="ACHKUC_000183"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO6616516.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11906..12715)
                     /gene="pabC"
                     /locus_tag="ACHKUC_000184"
     CDS             complement(11906..12715)
                     /gene="pabC"
                     /locus_tag="ACHKUC_000184"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO6616517.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSITAYPAHYDRLRNEGMMLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12835..14076)
                     /gene="fabF"
                     /locus_tag="ACHKUC_000185"
     CDS             complement(12835..14076)
                     /gene="fabF"
                     /locus_tag="ACHKUC_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO6616518.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14164..14400)
                     /gene="acpP"
                     /locus_tag="ACHKUC_000186"
     CDS             complement(14164..14400)
                     /gene="acpP"
                     /locus_tag="ACHKUC_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6616519.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14611..15345)
                     /gene="fabG"
                     /locus_tag="ACHKUC_000187"
     CDS             complement(14611..15345)
                     /gene="fabG"
                     /locus_tag="ACHKUC_000187"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO6616520.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15358..16287)
                     /gene="fabD"
                     /locus_tag="ACHKUC_000188"
     CDS             complement(15358..16287)
                     /gene="fabD"
                     /locus_tag="ACHKUC_000188"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6616521.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16303..17256)
                     /gene="fabH"
                     /locus_tag="ACHKUC_000189"
     CDS             complement(16303..17256)
                     /gene="fabH"
                     /locus_tag="ACHKUC_000189"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO6616522.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLATSEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17324..18394)
                     /gene="plsX"
                     /locus_tag="ACHKUC_000190"
     CDS             complement(17324..18394)
                     /gene="plsX"
                     /locus_tag="ACHKUC_000190"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO6616523.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVISSDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18475..18648)
                     /gene="rpmF"
                     /locus_tag="ACHKUC_000191"
     CDS             complement(18475..18648)
                     /gene="rpmF"
                     /locus_tag="ACHKUC_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO6616524.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18700..19221)
                     /gene="yceD"
                     /locus_tag="ACHKUC_000192"
     CDS             complement(18700..19221)
                     /gene="yceD"
                     /locus_tag="ACHKUC_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO6616525.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19420..20004
                     /gene="yceF"
                     /locus_tag="ACHKUC_000193"
     CDS             19420..20004
                     /gene="yceF"
                     /locus_tag="ACHKUC_000193"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO6616526.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20116..21075)
                     /gene="rluC"
                     /locus_tag="ACHKUC_000194"
     CDS             complement(20116..21075)
                     /gene="rluC"
                     /locus_tag="ACHKUC_000194"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO6616527.1"
                     /translation="MKTETPSVKIVAITADDAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21648..24833
                     /gene="rne"
                     /locus_tag="ACHKUC_000195"
     CDS             21648..24833
                     /gene="rne"
                     /locus_tag="ACHKUC_000195"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO6616528.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSTPVVEEVAEVVEAPVQVTEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25028..25981)
                     /gene="flgL"
                     /locus_tag="ACHKUC_000196"
     CDS             complement(25028..25981)
                     /gene="flgL"
                     /locus_tag="ACHKUC_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6616529.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVDGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25993..27636)
                     /gene="flgK"
                     /locus_tag="ACHKUC_000197"
     CDS             complement(25993..27636)
                     /gene="flgK"
                     /locus_tag="ACHKUC_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6616530.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSTADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNNKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27702..28643)
                     /gene="flgJ"
                     /locus_tag="ACHKUC_000198"
     CDS             complement(27702..28643)
                     /gene="flgJ"
                     /locus_tag="ACHKUC_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO6616531.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28643..29740)
                     /gene="flgI"
                     /locus_tag="ACHKUC_000199"
     CDS             complement(28643..29740)
                     /gene="flgI"
                     /locus_tag="ACHKUC_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6616532.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29752..30450)
                     /gene="flgH"
                     /locus_tag="ACHKUC_000200"
     CDS             complement(29752..30450)
                     /gene="flgH"
                     /locus_tag="ACHKUC_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6616533.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPAPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30503..31285)
                     /gene="flgG"
                     /locus_tag="ACHKUC_000201"
     CDS             complement(30503..31285)
                     /gene="flgG"
                     /locus_tag="ACHKUC_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6616534.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31457..32212)
                     /gene="flgF"
                     /locus_tag="ACHKUC_000202"
     CDS             complement(31457..32212)
                     /gene="flgF"
                     /locus_tag="ACHKUC_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO6616535.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDDIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32232..33437)
                     /gene="flgE"
                     /locus_tag="ACHKUC_000203"
     CDS             complement(32232..33437)
                     /gene="flgE"
                     /locus_tag="ACHKUC_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6616536.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSNPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33462..34157)
                     /gene="flgD"
                     /locus_tag="ACHKUC_000204"
     CDS             complement(33462..34157)
                     /gene="flgD"
                     /locus_tag="ACHKUC_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO6616537.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGIGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTATNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34169..34573)
                     /gene="flgC"
                     /locus_tag="ACHKUC_000205"
     CDS             complement(34169..34573)
                     /gene="flgC"
                     /locus_tag="ACHKUC_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6616538.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34577..34993)
                     /gene="flgB"
                     /locus_tag="ACHKUC_000206"
     CDS             complement(34577..34993)
                     /gene="flgB"
                     /locus_tag="ACHKUC_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO6616539.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35148..35807
                     /gene="flgA"
                     /locus_tag="ACHKUC_000207"
     CDS             35148..35807
                     /gene="flgA"
                     /locus_tag="ACHKUC_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6616540.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RIKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35883..36176
                     /gene="flgM"
                     /locus_tag="ACHKUC_000208"
     CDS             35883..36176
                     /gene="flgM"
                     /locus_tag="ACHKUC_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6616541.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36181..36597
                     /gene="flgN"
                     /locus_tag="ACHKUC_000209"
     CDS             36181..36597
                     /gene="flgN"
                     /locus_tag="ACHKUC_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO6616542.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITGKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36637..38172)
                     /gene="murJ"
                     /locus_tag="ACHKUC_000210"
     CDS             complement(36637..38172)
                     /gene="murJ"
                     /locus_tag="ACHKUC_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO6616543.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38282..39205)
                     /locus_tag="ACHKUC_000211"
     CDS             complement(38282..39205)
                     /locus_tag="ACHKUC_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6616544.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFLAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39207..39854)
                     /locus_tag="ACHKUC_000212"
     CDS             complement(39207..39854)
                     /locus_tag="ACHKUC_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO6616545.1"
                     /translation="MKYQLTAMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLASREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMLNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39865..40449)
                     /gene="rimJ"
                     /locus_tag="ACHKUC_000213"
     CDS             complement(39865..40449)
                     /gene="rimJ"
                     /locus_tag="ACHKUC_000213"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6616546.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMISEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40685..41893
                     /gene="mdtH"
                     /locus_tag="ACHKUC_000214"
     CDS             40685..41893
                     /gene="mdtH"
                     /locus_tag="ACHKUC_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO6616547.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKCFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41957..42604
                     /gene="grxB"
                     /locus_tag="ACHKUC_000215"
     CDS             41957..42604
                     /gene="grxB"
                     /locus_tag="ACHKUC_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO6616548.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42738..43298
                     /locus_tag="ACHKUC_000216"
     CDS             42738..43298
                     /locus_tag="ACHKUC_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6616549.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43404..44450
                     /gene="pyrC"
                     /locus_tag="ACHKUC_000217"
     CDS             43404..44450
                     /gene="pyrC"
                     /locus_tag="ACHKUC_000217"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO6616550.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44524..44769
                     /gene="dinI"
                     /locus_tag="ACHKUC_000218"
     CDS             44524..44769
                     /gene="dinI"
                     /locus_tag="ACHKUC_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO6616551.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10882 a        12140 c        11146 g        10744 t
ORIGIN      
        1 ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca ttggtcatac
       61 caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac cgggtgagat
      121 caaaaaacag agtttgttgt ttattatttg agcgaacttg tgagagtgat atagagtgag
      181 gggtgtatgg agggcattcg gtaggcctga taaaaggcgc atcaggcatt gatgctcgac
      241 tgtcggatgc ggcgtgaacg ccttatccaa accacagaat ccggagcgca tcaggtaccg
      301 gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct ttaatgcgct
      361 gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
      421 aataatgatg caactcttca gatgtccgct ggctgttaag cgcttcatca ttacgcgaca
      481 aaatcaccag gcaacgatcg cgattcttct cacggaagtt ggtcacacac ttagtggcaa
      541 tatcggcata ctcttccggg cgatcaatct tcccttccat gttctcataa gggaacaaat
      601 tggggttgaa gatcacctgg cggatatcac agagaaaacc aatccgctca gcccaataac
      661 cgcccagacc aacaccacaa atgagcgggc gctcgtcgac gttcagttgc aacattttgt
      721 ccacttcttt gagcaaatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatta
      781 gccgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggacagt
      841 tagagtcaaa accgtgcaaa tagataatca tcgcttcctc acataagcca gggttaatga
      901 cctgccttct cttcctgcca gcgttcatgt aactgattca gacgggtgct gatcgctttc
      961 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
     1021 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
     1081 ttgcagacca ggatcatatc gcaacctgca tccagtgatg cctgtccacg ctcggcataa
     1141 ctgcccataa tcgctgcgcc ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa
     1201 cccaattcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc cggacgcgga
     1261 tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
     1321 aaggaactga agaccgacat atctttagcg cgaatctccg cttgcggacg tgggtcgcac
     1381 ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgac ctgggaagtg tttcccggta
     1441 gttttcattc ccgcttcatg cataccatca ataaaacggc tggcaattgc cagggctttt
     1501 tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc gacgtccagc
     1561 actggcgcaa agctgatatc aatatccata gcaatcattt cactggccat caaccaacct
     1621 gcctcttgcg ccagttttcc gccttcttcc attcccaaca gcgcagcgaa tgattgtgcc
     1681 gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc ctgatctacc
     1741 gccaccacca gatgattgcg cgatgctgcg cggatctggc gcaccagttc acgtaactgg
     1801 gcaggatcat gatagtttcg cgtaaagaga atcagccctc ccaccagcgg atgcgccagt
     1861 atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac tggacccaca
     1921 ctgctctcct tattgtttta ttaatagctg ccgccacgtg tcatcggcca gcctgataaa
     1981 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag
     2041 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
     2101 ggcatagtca ttgaccaatt gccgatgctg gtcaatattt tccacccaca ccgccgccag
     2161 ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc
     2221 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg
     2281 tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccactg tccgccgcgc
     2341 cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc gccagccaaa
     2401 acgcggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
     2461 cgtttttacc tcgccgggca gatagtcgac caccatccag tcacggagat ataaatgcgg
     2521 cttcggtgca atgcatgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga
     2581 ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
     2641 gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
     2701 aaagcgcgac agcaattcgt cgcgcgtgag gggattattg ctgcgaaacg gcacctttac
     2761 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt
     2821 taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccaacattg cgggcaatgc
     2881 ctatggcttt actacgggtg cctaaactgt cctgcggcga taaacctaat tgttgcttag
     2941 ccattgacag ctgctgggcg gaaaccaggg taaatttacc gttattagcc agcgcatttc
     3001 gcagcgtttc ggtcgcttct gcggcattca acgaaccgtt agtacggttg ttaacgctat
     3061 caaccagcag gacgctaccc gcagtcacgc cgtcagcccc aagcatctta ctgaccatcg
     3121 gctgcattgc gccattccag tcataatgac gaatatgcgg cgcaggcggt gcagtttgat
     3181 cttcgtgctc aattgggcct ggctgctgcg gtatcgtcgg caccgatggc actgtgggga
     3241 caggctgttg tggctcagca ggttgttccg gcgctggttt cacttcttct accggtgcag
     3301 gttcacgttg ccccacacac ccggcaagaa acatcgccag cgcggtaatc aaggcgtagc
     3361 gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc
     3421 gcccccagaa aattggcgct gccgtacagc gtgaccgccg aatgtgcggg aatggtaacg
     3481 ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga
     3541 taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga
     3601 atatcagatg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac
     3661 tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc
     3721 agcactaaca acgccagaga catcagccca aagcatcctt ttctcatcgt tacagacctt
     3781 tatgcgtcag cattggtccc agcggacggc cacccaacaa atgcatatgg atgtggtaaa
     3841 cctcttgtcc gccatggcgg ttggtgttca tgatcagacg atagccatct tcggcaatac
     3901 cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct
     3961 cagctgacac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg
     4021 gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg
     4081 aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg acattttcct
     4141 ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat
     4201 ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt
     4261 tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt
     4321 ctctgtttgg tttacactcc aaatatgaat gcgtatattt ctcatttgca tttacacaca
     4381 aaaattattc gcacactaaa tataaccgct catcaccctg cttcggcatg agcctgacgc
     4441 caacataaac caagagattt caaatgcttt cgacacaatt taacagggat aatcaacatc
     4501 aagccatcat caaaccgtca ctccttgccg gttgcatagc actggcacta ttaccttccg
     4561 ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt tgagggttca gccacagccc
     4621 cagatgatgg cgaaaatgat tacaacgtaa cgtctacctc tgcgggtaca aaaatgcaga
     4681 tgactcaacg tgatattcct cagtcggtca ctattgttag ccagcagcgg atggaagatc
     4741 agcagttaca aacgctgggc gaagtgatgg aaaacacgct ggggatcagc aaaagtcagg
     4801 cggattccga tcgtgctctt tattattccc gcggattcca gatcgataac tatatggttg
     4861 atggtatccc cacctatttt gaatcgcgct ggaatctggg cgacgcactt tctgatatgg
     4921 cactgtttga acgcgtagaa gtagtgcgtg gcgcgacagg cctcatgacc gggacgggta
     4981 atccatctgc ggcaattaat atggttcgaa aacacgcgac cagtcgtgaa tttaaaggcg
     5041 atgtctcggc ggaatacggt agctggaaca aagaacggta tgtggcggat ttacaaagcc
     5101 cactcaccga agacggtaaa atccgcgcac gaattgtcgg cggctaccag aataacgact
     5161 catggctgga ccgctacaac agtgaaaaga ccttcttctc cggcattgtc gatgctgatt
     5221 taggcgatct tacgatgctt tcagccggtt acgaatatca gcgtattgat gttaatagcc
     5281 caacctgggg tggtttaccg cgctggaata ctgatggcag cagcaacagt tacgatcgcg
     5341 cacgcagtac cgcacctgac tgggcgtaca acgataaaga gatcaacaag gtctttatga
     5401 ccctgaagca gcggtttgct gatacctggc aagcgacgct gaatgccacc cactctgaag
     5461 tcgaatttga cagcaaaatg atgtatgtcg atgcctacgt aaacaaagcg gatggtatgc
     5521 tggttgggcc atacagcaat tatggacctg gctttgatta tgtcggcggc accggttgga
     5581 acagcggcaa acgtaaagtt gatgcgctgg atctgttcgc tgacggtagt tatgaattgt
     5641 ttggtcgtca gcacaattta atgtttggtg gcagttacag caaacaaaac aatcgttact
     5701 tcagttcatg ggccaacatt ttcccggatg aaattggcag tttctacaac tttaatggca
     5761 atttcccaca aaccgactgg tcaccacaga gcctggcgca gaacgatacc acacatatga
     5821 aatcgttata tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg
     5881 cacgttatac caactggcga gttgatacgc tgacttacag catggagaaa aaccacacca
     5941 cgccttatgc tggactggtg tttgacatca atgacaactg gtcgacctac gccagctata
     6001 cctctatttt ccagccgcaa aatgatcgtg acagttcagg caaatatctg actccaatca
     6061 ccggtaacaa ctacgagcta ggtctgaaat cggactggat gaatagccgt ctgaccacca
     6121 cgttagccat cttccgtatt gagcaggata atgtcgctca gtccaccggt acacctatcc
     6181 ccggcagcaa cggcgaaacc gcctataaag cggtggatgg gacagtcagt aaaggtgtgg
     6241 aatttgaact caacggcgca attaccgaca actggcagtt gacgtttggc gcaacgcgct
     6301 atattgccga ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc accacgttta
     6361 aaatgttcac cagctatcgg ttgcctgtca tgccagagtt gacggtcggc ggtggtgtta
     6421 actggcaaaa tcgcgtgtat accgacaccg tgacgccgta tggcaccttc cgcgccgagc
     6481 aaggtagcta cgcgctggtg gatctcttca cccgctacca ggtgacgaaa aacttctcgt
     6541 tacaggggaa tgtcaataac ctgttcgaca aaacctacga taccaacgtg gaaggttcta
     6601 tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac gtatcaattc tgaataacac
     6661 ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca acgtcttacg gattagtggt
     6721 tacggatgta ctcatccatc tcggttttca ggttatcgga tttagtaccg aaaatcgcct
     6781 gaacaccaga accagcaacc actacgcccg ctgcgcccag tttcttcagg ccagcctgat
     6841 ccactttaga cacatcagca acgctgacgc gcagacgggt aatacatgcg tcgaggttag
     6901 taatgttttc tttaccacca aatgcagcaa ccagagccgg tgccatttcg ctggtacctg
     6961 tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt tttcagatcc agtgctttaa
     7021 tcagcacgcg gaagatggtg tagtaaacaa tggcataacc gataccgacg atcgggaaca
     7081 gccacagttt gctgctgtta ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg
     7141 acgtaccgtc acgcatcccc agaagaatac agattgggaa tgccaggcct gccagaatcg
     7201 cgtggatgat gtacaggatc ggcgcaacga acatgaagga gaactcgatc ggctcggtga
     7261 taccggtcag gaacgaggtc agcgccgcgg agatcataat accgcccact ttcgcgcggt
     7321 tttctggttt agcagagtgc cagatagcaa ttgcggcagc tggcagaccg tacattttga
     7381 acaggaagcc accagacagt ttacccgcag tcgggtcacc cgccatataa cgcggaatgt
     7441 cgccgtggaa aacctgacct gctgcgttgg tgtattcacc aatctgcatc tggaaaggta
     7501 cgttccagat gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc
     7561 caaacgcaac taccgggttc tggtaagcag cccactgaga gaaggtctgg attgcagaac
     7621 caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc aggcctgaaa
     7681 tgatcggcac aaagcgttta ccggcaaaga agccaagata ctcaggcagc ttaatacggt
     7741 agaaacggtt aaacatgtac gctgcgatcg caccggagat aatccccccg agtacgccag
     7801 tatccgccag gtgtttagag gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa
     7861 ccacggccat ggttttaacc atgatgccat aggcaacaac tgcggccagc gcggatacgc
     7921 catcgttatt ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa
     7981 agacggaacc gcctgcttct gccataacat gcgatacaac ggcgggcagc cagctgaaat
     8041 tcgcggaacc gacgcccagc agaatacctg cgataggcag tacggatacc ggcagcatca
     8101 gcgatttacc gaccttttgc aggttagcaa atgcattctt aaacataatt gagagtgctc
     8161 ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa ggggggagtg cctcgccgtg
     8221 tacagggcat ctaagcgccc tttatttatt acacagagta aaataattca gtgccgattt
     8281 gtttgacggc tatcacgttt caacttcaga tcattggaga agttgcacat tttgacaaaa
     8341 catatgtcac aatatttcaa acgtggttat tcaccgcccg attttcggcg gtgaacttta
     8401 ctcgttttag ccattaatta cgagctttaa aaaaactcat tcaacggatg gattgaaggc
     8461 gggaagcgtc gatgtgaaac agacgggcga agttatcggt ggttacctgc gccagttctt
     8521 caacggcaac acctttcaac acagccatgt attctgcaac gtcacgaacc atcgcaggtt
     8581 gattctcttt tcctcgatgc ggtactggcg caaggtaagg tgagtcagtt tccaccagta
     8641 accgatccag ggggacataa cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca
     8701 caatgccgga aaaggagatg taaaatccga gatccagtaa tttgcccgcc gtttctctgt
     8761 cctctgtaaa acagtgtagt acgccaccgc aatccgtcac tttttcttcg cgcagaattg
     8821 ccagcgtatc ggcgcgggcg tcacgggtgt ggacgataac cggcttgttc agttcacgac
     8881 caatctggat atgatggatg aaggactctt gctgacgtac tttagtttcc ggcgtgtaat
     8941 aataatccag cccggtttca cccagcgcta caacgccctc ttctgccgcc agacgacgta
     9001 aatcttctac atcgtaggga tcattctggt tgagtggatg cacgccacaa gaaaatacga
     9061 cgttgtcgcg ttcgcctacc agatcccgca tgtgtaaata acccggtaat gttgtggcga
     9121 ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct
     9181 tatgcaaaga ttcataatcc agaccatcga gatggcagtg tgagtcgact aaaaacatga
     9241 tgtctctctt aaagatgagg aaccggtagc acaacgcccg gttgcaggta atgctcaata
     9301 cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc ctgtgacaga cattaactgt
     9361 tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc gcgagggaga aagatggttt
     9421 gccagttcga cgaccaggcc cggcacatca acattggtca cctgcgcagc gccatgatgg
     9481 cgttttagcg catccatcag caacgttgcc agccagtgta aacgtgccgg agcttgttca
     9541 tgattaaggg ccgctagcag cgaataccag tcgcccgatt gcacgctata tgccagcgcc
     9601 tgacacaatg tttcacgagc ctgccagtta tctccctgaa acaacgccag tgccgcccca
     9661 ggcgaaccgg cgcttaagcg caatgcggca agtagtgcat cctgtgacat tgtcacttcg
     9721 cgtgaaagcc agatcacggc gtactgttcc ggcggcggcg caaggtaatg taaccgacaa
     9781 cgactacgta atgttgccag taaacgttct ggctcgcggg tagcaaggaa aaaccacgtt
     9841 tctgctggtg gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc ggttagtaag
     9901 gcagcatcgg ttacccagac aactttcgca ccacctaagc gtgcgtgctc attcagcttt
     9961 tcggtgacct cacgtaccgc atcaatgccc agcgcatttt tccctttttc gggagccagg
    10021 gtgtagtaat cgggatgtgt gccagcctgc atcaactgac atccacgaca gtgaccgcaa
    10081 cttttgtggc cctgcggttg ttggcagagt aaataacggc tcagggcgta gattaaagca
    10141 tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct tccggcctga
    10201 tagctggcta ccagtttttc gaaatcaggt cgtaaccatg gataccatct catgcgtcca
    10261 actccttcac ccagttggtc acggtagtgc ggattgcatc catcacggcc tccagcggct
    10321 gggtggcatc aatggtatga atgcttttat cttgtgctgc cagttccaga tagcgggcgc
    10381 gggtgcgatt aaagaaatcg aatgactctt gctcaatacg atccagctca ccacgggcgc
    10441 gagcacgttt taagccgacc tctggggtga catcaagata aagcgttaag tcagggcgaa
    10501 aatccccgag aacagcatca cgcagtgttg ccagcatatg ttggtcaata cctcgtcctc
    10561 caccctgata ggcttgagtg gagagatcgt ggcgatcgcc aatcacccag gtgccgttag
    10621 ccagcgcagg tttgatgacc gtttctacca gttgaacgcg cgcggcataa aacatcagaa
    10681 cttcggcttt atcggtaatg acttcatcgc ctaccgattt gatatccagc accaggcttc
    10741 ttaacttttc ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg tcgcggatac
    10801 ccagttgctc aagcgtctca accaccacgt tacgcgcggt agttttgcct gcgccttcca
    10861 gcccctcaat gacgatatac ttactgcgca tttttttcct taagcacttt cagataatcc
    10921 tgcacagact tgttatgact ggcaagattg gtattaaacg tgtgaccacc tttaccatcg
    10981 gccacaaaat agagatacgg cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc
    11041 cccggcgtcg ctatcgcacc tggcggcaga ccggtaatgg tataggtgtt atacgctgtc
    11101 ggcgtttcca ggtctgcacg agaaagtttg ccattataac gctctcccat cccgtaaatc
    11161 acggtcgggt cggtctgcag gcgcatacca atgcgtaaac ggttgataaa tactgaggca
    11221 acctgatcgc gttcactggc aacggcggtt tctttttcga taattgatgc catcgtcacc
    11281 aactggtttt tatctttata aggcagaccg tccgcacgcc cttcccaggc gctatcgacc
    11341 gctttcacca ttttcttgtg cgctcgcttg agtaacgcga catcggtggt attggcggta
    11401 tacatccagg tgtctggcca gaaccaacct tcaatccact ccgggttttc cagttcaagt
    11461 gcctgcgcta cggtggcgta cttatcgtcg ctcagcgtgt gcttgatata cggagcctca
    11521 cgcaattgct tgaggtagtc gctcagacgc atcccttcta ccagtcgcag cgggaactgt
    11581 gcttctttac cgctttccaa caatttcagc atctcgcgca cggtcatctg cggtgtaaag
    11641 cggtaagtcc cggctttaaa gtgagaaaga tccggttcga tacgcagcaa ccattgaaaa
    11701 acccgtgggc gattgatgat cttatcggca taaagctgtt cacctagcgc cagacgtccg
    11761 gtccctggct tcagggtaaa tatcgtctct tctttgataa gcaatttgct gtcggcaaga
    11821 tggcgaacct tccagacgcc cacaccagcg gcgataccca gtaccaccag caataacaag
    11881 attatcaata acactttttt catgactaat tcgggcgctc acaaagtggg gctaaatatt
    11941 catataacgt tgctgacgaa aaggagacat cgccacaggc acgtacgggc atcactggca
    12001 ttaacgcatt acaaataacc atctcatctg cctgcaacgc ctcttccaaa gaggcttgca
    12061 cttcgacaag ctgataagag gattgtgcca gcaaacggat acagaattgt cgcataatgc
    12121 cgttaacacc tgcctgatcc agtcgcggcg tataaactac gttacccttc cgccagaaca
    12181 aattagccgc acagcattcc gtaacccacc cttcgctgtc aaggaccagc gcctcatcag
    12241 cgtttgtctg ctcaagatga gagcggatca atacttgctc aagccgatta agatgtttaa
    12301 tacctgcaag atgaggattg cgccccagcc gcaccgggct tagcgccaac atcatccctt
    12361 cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaat ggagagaatc cgcgttgccg
    12421 gtccactgtt caatgtgctg taccctcgcc cgccactacc gcgactgatc acgactttca
    12481 gcacaccatt ctgctgttct gctgccagcg ttttcatctc ttgttcaagc tgaggccaga
    12541 agtcacagga aatcatcaac cgctgacaag catcctgtag tcgctggata tgcgccgata
    12601 acaaactgac tttaccgtcg ataactctgg cggtggtaaa acaaccatca ccaaactgcg
    12661 ttgcccgatc gcttactgcc agcgattcct gcttatgacc gttaattaag aacataccgg
    12721 ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag tcggaataaa
    12781 agctaagaaa aaaggcccgc aagcggacct tttataaggg tggaaaatga caacttagat
    12841 ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac acagagtgta
    12901 ttccattccg ctaacctgac gcgcttcgtg cggtacgaaa tccagatcgc aaccttcgtc
    12961 cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca ggatggagta
    13021 gatagattct actgcacccg ccgcacctaa caggtgaccg gtcatagatt tcgtggagct
    13081 taccaacacg cggcttgcag cttcaccgaa gatggttttc accgcctgcg cttcagcttt
    13141 atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac ttgcttcaat
    13201 gcctgcatca cgcagagcat ttgccatcgc cagagctgcg cctgcgccat tttctggcgg
    13261 tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag cgtaaatttt
    13321 cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg caccatcgcc
    13381 cagtacgaaa ccatcacgct ctttatccca cgggcggctc gccgcttgcg ggttatcatt
    13441 gcgggtagac aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg tactggcttt
    13501 ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac gcgcagcatg
    13561 gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg ggccacgcag
    13621 gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg gaacgaagaa
    13681 tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt cgatcagtcc
    13741 gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct cttccgttat
    13801 ttcaaggcca gaatcctgca tggcctgaac gccagcgaca attccatatt gaatgaaggc
    13861 atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat cctttactaa
    13921 gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc tgatgccact
    13981 ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag gagacaacat
    14041 gcccagtccg gtcacaacta cacgacgctt agacacgttt gtcctccagg gagggaaaaa
    14101 atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc tggagatgtt
    14161 cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg tgattttctc
    14221 agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta ccagctcaac
    14281 ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg taacttcttc
    14341 ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga tagtgctcat
    14401 actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt
    14461 gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat tttgaggcct
    14521 taccccccaa taacgcaaat cattttgcac taattattac tcataaccac gcagactgcg
    14581 cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc cgttcacatg
    14641 caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc
    14701 gatttcctgt gcgccgccga ggcgacccgc aggaacctgc gccaggatac ccgcacgctg
    14761 gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa caacgtttac
    14821 agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc
    14881 tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca cagaaccgat
    14941 agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt ttgacagacg
    15001 gaaaacagat gaaaggttgg tttcgataat atcgttccac tcttcatctt tcattcgcat
    15061 taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt caccaaattc
    15121 tgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat tcaacatcag
    15181 acctttgccg ttggcaccta aataatcact gatcgcctga gcgccatttt cactggtcgc
    15241 agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc ggccaattcc
    15301 gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgattt tcctctttta
    15361 aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc
    15421 gacaatgcgt ttcgtcaggc cagtaagcac tttgcccggg ccgacttcat agagatgttc
    15481 tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt tatacaactg
    15541 acgtaccagt gcgtcacgga tggcatcacc attggtttcg catttcacat caacgttatt
    15601 cacaacagga actgttggtg cgttaaaggt gattttcgct aattctactg ccagtttgtc
    15661 ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg gcagagcgcg
    15721 ttttgcgccc gcagctttac aggcagcgcc agcacgctca accgcttctt tatgaccggc
    15781 aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac cttctgcagc
    15841 ttcttcacac gcttttccaa tagacgcatc atccagaccg atgattgccg ccatagcgcc
    15901 cgttccttcc ggtacggctt cttgcatgaa cttgccgcgc atctcaacca gacgcaccgc
    15961 atcagcgaaa tcaatcacac cagcgcaaac cagcgcggag tattccccca ggctgtgacc
    16021 ggccatcatt gccggtgctt taccgccctg ctgctgccat acgcgataca gcgcaacaga
    16081 tgcagtcaac agcgctggct gagtttgcca ggttttattc agttcttcag ctggcccctg
    16141 ctgggtcagc gcccacagat cgtagcccag cgccgcagaa gcttctgcaa acgtttcttc
    16201 gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag aaccctgtcc
    16261 agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc
    16321 ggagccccag gtgaatccac cgccaaaggc ttcaagcaga accaactgtc ccggcttaat
    16381 gcgcccgtca cgtacagctt catccagcgc gcacgggaca gaggccgcag aggtattacc
    16441 gtggcgatcc agcgtcacca cgacattgtc catcgacatg ccgagttttt tcgccgttgc
    16501 actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag aacggtcaag
    16561 attattcgcc gccagcgtct catcaacgat gtgcgccagt tccgttaccg caaccttgaa
    16621 gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacac ggtcagcatt
    16681 aggcagcgtc agcaattcac catagctacc atcggcatgc agatgggtgg agatgattcc
    16741 cggctcttca gaggtagcca gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt
    16801 cccacgatct gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga ccagagcata
    16861 cttcaccgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg tgaaacctgc
    16921 gcaggctgct gcaacgtcaa atgccgggca acctttaatg cccaacatgc tttgaatctg
    16981 acaagctgcg ctcgggaaag cgtgcgtcgc agaagtcgtt gccacaacga tcaagccaat
    17041 ctggtctttc tcaatacccg ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt
    17101 tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat
    17161 ccactcgtca gaggtatcca ccattttttc caaatcggcg tttgtccgca cttgttcggg
    17221 cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac ttttcggtta
    17281 tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc
    17341 ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg cgagcggcaa
    17401 ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag
    17461 ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct aacagacagg
    17521 cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc
    17581 aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga gatttcagca
    17641 gagaaaggaa catcctgaca acaccttcca tcgtctttaa tgtgacattt cctgtaaagc
    17701 cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca agatagccga
    17761 tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata ctgtcgagac
    17821 cctttacttc ttcttcacca atattgagca acgccacgcg aggattagga atttccacca
    17881 cctcttcagc cagaactgag cccataatgg caaattgcac caacattgta ctgtcacaat
    17941 cgacgttggc ccctaaatcg aggaccaccg ttttgccctt ttgctgatgc ggtaataccg
    18001 tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt gccagcccca
    18061 tcagcgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct ttcaccagct
    18121 ccagggccac gcgcattgaa ctcccacgac tggcgcggat agcttgcgaa ggccgggcat
    18181 cactggagat aactgactgc gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag
    18241 ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga gtgagttgcg
    18301 aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaagggcca aaatcccctc
    18361 ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct
    18421 ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc gtgattactt
    18481 agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt
    18541 ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt catgggaacg
    18601 acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg accttactcc
    18661 tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct ttaagctggc
    18721 taatacggca aatgggtttg gcttttgcgc ttcttcaggc agttcaccaa agaccatgtc
    18781 cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca aggcgaggat
    18841 gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa cctcaatcgg
    18901 ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac taaaacaata
    18961 cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga gcgttaccgt
    19021 caccttcgca tcgccattta acactgcgag acgttggtta tcgatagcga acgacatgga
    19081 gcattccaca tcactgtcca cactgactac ggattcggcg acgcgctcaa cctgatcagg
    19141 agtatagata ccctggtaat caaggcgttt ctgagccgta cgaaccggat cgagagtcag
    19201 gggtaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca tagagtcaaa
    19261 gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg ggttactgct
    19321 ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc atagtttaaa
    19381 atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact tattttagcc
    19441 tccacctcgc cctggcgtcg cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca
    19501 gcacccgagg tcgacgaaac tccacgcagc gacgaatcac cgcgacagtt ggtgcttcga
    19561 ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt aattattggt
    19621 tcagaccagg tatgtgttct tgatggcgaa atcaccggta aaccgttaac ggaagaaaat
    19681 gcccgtctgc aattacgcaa agccagcggc aatatcgtca ccttctatac tggactggcg
    19741 ttgtttaatt cggcgaatgg gcatctacaa acagaagtgg agccttttga cgtccatttc
    19801 cgtcacctga gcgaggcgga gattgataat tacgtgcgta aagagcatcc cctgcactgc
    19861 gcgggtagct ttaagagtga aggatttggc attacgctgt ttgagcgttt agaggggcgt
    19921 gatcctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa
    19981 gggaaaaacc cgctgatggg atagtttttt gcgccggatg cggttacttc gcatccggca
    20041 atcggtgctg gcggcctgat gcggcgtaaa cgccttatca ggccaaccgc ccattatcag
    20101 gtttatatgc ttgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc
    20161 atccatcggc gcttcaatac gcatcacctc accggtcccc ggatgagtaa acttcaacgc
    20221 tgcggcgtgc aggaacaggc gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa
    20281 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatatt gtgtatgcac
    20341 acggatctga tgagtacgcc ctgttaccgg gctacaacgc accagggtgg caaatgcata
    20401 gcgctcttcc actttaaagc gtgtttccga cggtttgcct tcctgactca cacgcacgat
    20461 acgttcgccg ctttgcagaa tatttttcag taacggcgct tggacgctct tcacatgcga
    20521 ctgccactga ccgcgcacca gcgccaggta atctttttgc atcccttttt cacgtaattg
    20581 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac ctgaggtgtc
    20641 ccggtcaaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    20701 ttcaataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    20761 cagcaccagg atatgatcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    20821 cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg ggatacgcac
    20881 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    20941 gcctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    21001 aaagttgtcg atacgttgcc ccgcgtcgtc agcggtgata gcaacaattt ttacggatgg
    21061 agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    21121 tctatgctta tatttagttt tacaccttat tacttcacta ggtgatcact ttattgatgg
    21181 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    21241 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    21301 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    21361 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    21421 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    21481 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    21541 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    21601 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    21661 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    21721 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    21781 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctgaacgtc
    21841 acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac tacagtgctc
    21901 atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg
    21961 ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt ctggcgggta
    22021 gctatctggt tctgatgccg aacaacccgc gcgcgggcgg tatttctcgt cgtatcgaag
    22081 gcgacgaccg taccgaatta aaagaagcac tggcaagcct tgaactgccg gaaggcatgg
    22141 ggcttatcgt gcgcaccgct ggcgtcggca aatctgctga ggcgctgcaa tgggatttaa
    22201 gcttccgtct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc ccggctccgt
    22261 tcctgattca tcaggagagc aacgtaatcg ttcgcgcatt ccgcgattac ttacgccagg
    22321 acatcggcga aatccttatc gataacccga aagtgctcga actggcacgt cagcatatcg
    22381 ctgcattagg ccgcccggat ttcagcagca aaatcaaact gtacaccggc gagatcccgc
    22441 tgttcagcca ctaccagatc gagtcgcaga tcgagtccgc cttccagcgt gaagttcgtc
    22501 tgccatccgg tggttcgatt gttatcgaca gcaccgaagc gttaacggcc atcgacatca
    22561 actccgcacg cgcgacccgc ggcggcgata tcgaagaaac cgcgtttaac actaacctcg
    22621 aagctgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta
    22681 tcgacttcat cgacatgacg cctgtacgcc accagcgtgc ggtagaaaac cgtctgcgtg
    22741 aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc tttggcctgt
    22801 tggaaatgtc ccgtcagcgc ctgagcccat cactgggtga atccagccat cacgtctgcc
    22861 cgcgctgctc cggtaccggt accgttcgtg acaacgaatc gctgtcgctc tctattctgc
    22921 gtctgatcga agaagaagcg ctgaaagaga acacccagga agttcacgcc attgttcctg
    22981 tgccaattgc ttcttacctg ctgaatgaaa aacgttctgc ggtaaatgcg attgaaactc
    23041 gtcaggacgg cgtacgctgt gtgattgtgc caaacgatca gatggaaacc ccgcactacc
    23101 atgtgctgcg cgtgcgcaaa ggggaagaaa ccccaacctt aagctacatg ctgccgaagc
    23161 tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt aagcgtccgg
    23221 aacaacctgc gctggcaacc tttgccatgc cggatgtgcc gccagcacca acgccagctg
    23281 aacctgccgc gcctgtcgta gccccagcac ctaaatctgc accggcaaca ccagccgctc
    23341 ctgcccaacc tgggctgttg agccgcttct tcggcgcact gaaagcgctg ttcagcggtg
    23401 gtgaagaaac caaaccgtcc gagcaaccaa caccgaaagc agaagcgaaa ccggaacgtc
    23461 aacaggatcg tcgcaagcct cgtcagaaca accgccgtga ccgtaatgag cgccgcgacc
    23521 ctcgtagtga acgtactgaa ggcagcgata atcgcgaaga aaaccgtcgt aatcgtcgcc
    23581 aggcacagca gcagactgcc gagacgcgtg agagccgtca gcaggcagag gtaacggaaa
    23641 aagcgcgtac caccgacgag cagcaaacgc cgcgtcgtga acgtagccgc cgccgtaacg
    23701 atgataaacg tcaggcgcaa caagaagtga aggcgctgaa tgttgaagag caatctgttc
    23761 aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc
    23821 tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg
    23881 ttgaagaaac tgtcgcagcc gaaccaattg ttcaggaagc ggcagcccca cgcacagaat
    23941 tggtgaaagt tccgctgcca gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca
    24001 atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc
    24061 tgcgcgtaag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca acccagtcgc
    24121 caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc
    24181 gctatccaat tgtacgtccg caagatgtac aggttgaaga gcagcgcgaa caggaagaag
    24241 tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgccgaa cctgttgtta
    24301 gcacgccagt tgttgaagaa gtggctgaag tcgtagaagc ccccgttcag gttaccgaac
    24361 cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat tgctgccgcg gtaactgaac
    24421 agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagttgca gaacacgcag
    24481 aaccggtggt tgaaccgcag gaagagacgg cagatattga agaagttgcc gaaactgctg
    24541 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    24601 tcacagcaga agttgaaacg gtagctgcgg ttgaacctga ggtcaccgtt gagcataacc
    24661 acgctaccgc gccgatgacg cgcgctccgg caccggaata tgttccggaa gcaccgcgtc
    24721 acagtgactg gcagcgccct actttcgcct tcgaaggtaa aggtgccgca ggtggtcata
    24781 cggcaacgca tcatgcatct gccgctcctg cgcgtccgca acctgttgag taataattag
    24841 ctcaagtaat caagccctgg taactgccag ggctttttta tttcatcttt gagtcaacat
    24901 attaaaattc atcaaatggc atccttgcta accaacaata caaaataggc aaaaaaatgc
    24961 ccgccccggc gatcccggag cgggcagaca aaacataccc agtttcatga tatgttttaa
    25021 agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat
    25081 acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca
    25141 catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaatgaat
    25201 ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaacagtc agcacattgt
    25261 tcagcgagtt tttcagtccg cggttggttt tatccagtgc cgcagcggcg gtttctttat
    25321 ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcgctatcc agcatggcaa
    25381 aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttgctg gtaatactgt
    25441 cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg acctgttgct
    25501 taatactttc tgttccgccg acataatcac cgtcaacctc actaaacggc gcagtctctg
    25561 ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc agattcagca
    25621 actggtcacg aagcccctga atatccgtag ccagcgaggc ccggtcatcg tcactcaagg
    25681 tgccattgct ggcgtaaaca attttttcct gggcattctg gatagcagtg gtgacctggc
    25741 taagtacact ctcttccagt gacacttttt gagtggcgaa agtacgcgcc agcgtgtact
    25801 ggctgttttg cgcctgtgcc tgggagagaa caacagcttg tgatgcagca atgggatcat
    25861 ctgaagggtt aacgactcgc ttacccgtcg acatctgttc gccgtacttc atccattctg
    25921 cctgagaatt ggtgatgcca cgcatgtttt gctggtacat catctgggta ctgaaacgca
    25981 ttttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag
    26041 aacctgcgca ttcgcgaggt aatactgctg aaaacgttgc agatttccgt actcctcatc
    26101 gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca cattaccttg
    26161 cgtggcgcta ctggttttca acgtcgcggt tttattcccg atatcactca ctaatgaggc
    26221 ataagcgtcg ttaaaggatt tcgcaccgcc caccgtttta ttgttgcttt gcagatccag
    26281 cagggcctga ccgttgcggt tgtcgctatc acccgcatct tctgcgctcg ccatggcgat
    26341 tttcgcttcg tcggtgatta atacatccat gttgacgatg gcgtcactta ccggtttcag
    26401 tgtgaagctg tcgttaacgg caggcgttcc tgtaaacgtc aactccagac catcaaatgc
    26461 cactttaccg ttggcatccg gcgtcaccgt aaaagtggta ttactggcaa ggcgggtgac
    26521 ctgccactga ttattatcga acgagatttt gtaatctgtc gccagtaccg cggaggcatc
    26581 agttaccgtg gcaccgatcg caacgtcacc gttgtttttc gtgttttgca gtaccgcggg
    26641 cttaccgata gcaaagaaat cttcaccggc atcgccgtta gcatcaaatc cggctttgtg
    26701 ttgggtgttg aaagcctcgg caaatgccag cgccagttgt ccaagcgtat tacgcgtctg
    26761 gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat tcagtaattt
    26821 ctccgggatc tcaatattgc cagccgtccc atcaatataa gcgacagtcg tacgcgaagg
    26881 gtcggcggtg gaaggaactg ccgccagctg ccgcgccgta cttccctgaa ccagtgagta
    26941 gccattggcc atcgtgatgt tataagtgcc accatcctga acgctaactt ctacgccaac
    27001 aatctggttt aattcactca ccagttgatc gcgttgatcc agcagattgt taggtgacgc
    27061 ccctgccccc acgcctgtca gacgcgagat ttgatcgttc aggctggcga tttgtttagc
    27121 gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc
    27181 gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag
    27241 cgcctggcgc gctgccgggt cttccgcgtt acttaccagc gtttgcaggc tggtgaagaa
    27301 atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga
    27361 catctgctca tagcgggcag tcaggccgct actttgcgtc tgcgccgcac gtaactggtt
    27421 ggtaataaat gcatcatact cacgctgcac accagaaacg tagacgccat tgccaaccca
    27481 gccgccagcg cccagcgtgc tattggcctg cgccataata gtggtttggc gggtatatcc
    27541 ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt
    27601 cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat
    27661 tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg
    27721 ttcatactgt aggttttgct cactttgtcg cttatcgatt tcatctgctg aatcatgttg
    27781 gtgagtttgc gggcatagtg aggatcggtg gcatagcccg cgtcctgtag ggcctgcgcc
    27841 ccctgctccg cactcgcggc ggtcgtcacg gcagcgtagc gcggattacg tgttaacagc
    27901 ccaacgtaat ccgacaatgc ttccagatac gagctgtaga cccgaaattt cgcttttact
    27961 ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtgactgg ccctttccag
    28021 ttgccagagg ctttgacgcc aaacaggtta taactcggct cgccgttttc acggcggatt
    28081 tgccgttgtc cccagccaga ttccagcgcc gcctgagcga ggatcaaatg atgtggcaca
    28141 ccgctttgct ggcttgccag ttgggcgggc aacgagagtt gcgcgaggaa tgctttactg
    28201 tcacccggca gcgaatcatc gtagttacgt ggtacggcct tttgcaccag ctgcgaaagc
    28261 gtctgatttt gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg
    28321 gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc
    28381 agacctttgc ccgccgtcat ctgttgggca atctgctggt catacatact ggtatacagg
    28441 cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac
    28501 atcatctgca cgaacatccc ttccacctgg cgggccaccg gacggatatt tgccgctgga
    28561 tcttcgcccg ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc
    28621 agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac
    28681 tttgcattga ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg
    28741 cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct
    28801 ggcgtaaatc gatctgcgtt tgtggagtaa ccacagtctg tccaccacca aacggtgtat
    28861 ctggctggct gacattggcc tgacgattga ccgtaaccga gagattcccc tgcgctactg
    28921 cgcagctgtc cagggtcact tcgcgattca tcaccaccga gccagtgcgc gagttaatca
    28981 ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatcg gcaaggaaac
    29041 ggacctggga actgttgcca cttggtacgc gcacctgaat agtccgcgca tctaacgcgg
    29101 tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga
    29161 aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt
    29221 cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggttc acctgaacac
    29281 tgctaccgcc agcggaggct ccagcgccac caaccagaat attgccctgc gccagcgcat
    29341 acacctgact gtcaacgccc ttaagcggtg tcatcaacaa cgtaccgcca cgcaagcttt
    29401 tggcattacc catggaagaa accaccacat cgatggtttg cccctggcgt ccaaacggcg
    29461 gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg
    29521 gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct
    29581 gggttgtctg gtcaccggtt ccatccagcc ccaccaccag gccatagcca atcagtgagt
    29641 tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtga
    29701 taaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg
    29761 cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc
    29821 attgcctacg tattcaatgc gcgcatccgc cacctgagta gatggtacgg tattgctgcc
    29881 gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac cctgattaat
    29941 ggcgatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt
    30001 caccgtcaac gtaccgctaa aggtattgct ggcattggcc ccgccttttc cgttgaacgt
    30061 gttaccaccg gaggcttcga catcggcacg agcgttacca aacaacccct gcaaatagcg
    30121 cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct
    30181 tttgctggcg ctgacgttct cctgcaacac gatagtcagc gtatcgccaa tattgcgtgg
    30241 tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat
    30301 agacccgttg gcgacgggcg tcggaccggg agccggttgt gcactggtcg ccccttgtac
    30361 cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat
    30421 ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg
    30481 tgaacctgcc accggttaag ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg
    30541 tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt
    30601 cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt
    30661 tcaggcccgg cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt
    30721 cgccaatgct ctccagtccg gtgtcattca taaaggtggt gagattgagc tgcccaacct
    30781 gaaccggagc tgcctggcct tgttgggtta cgctgaccac accatcacga ccgatggtga
    30841 tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccggccgtca
    30901 ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct gatgagccat
    30961 ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacgtcttta ctgttattgg
    31021 tttgcgacag gttgccctgg ctgtgtaaac gttccgtggc gaccgggcgt acccccgtgc
    31081 cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa
    31141 tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga
    31201 cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt
    31261 tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattagtga caaagtgcat
    31321 atgtcttctt gccggatgcg gcgtaaacgc cttatccggc ctacaagtgc tcgcaaaatc
    31381 gataaattgc gattgccccc atagacccga taaacttgcg catagggcaa cttatacttg
    31441 tcatgtatcc tttcaattaa ctcatcgaca gcagttggtt ggcacggcct gcgttatcat
    31501 cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca atcatgtcgc
    31561 tcattgccgc aacggcattg acgttactgc cttccagaac acccgacatc acacgcaagg
    31621 ttggatctgc ctgcagtacc ggcccacgcg tagcctggct ttctgcgctt aaacgaaaaa
    31681 tgtcatcgtc accgcgctgg acttcgctgc ccgtggcttt caccagcttc agacgcccca
    31741 ctggcgcaac cgtatttgcc ggatcgcccg gattcagcgc cgagattgtg ccatcggcag
    31801 caatagtgat ttccgcccct tccggcacag caattggccc cgcctcgcct accaccggat
    31861 gtccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta cgcgtatacc
    31921 cttcactacc gtcagcggtc tgcacgacca gccaaccatc ctgctgcaac gcaacgtcca
    31981 gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg
    32041 ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg cactgcgcgc aaggcgttta
    32101 actgcgcgcg aaaaccgggc gttgaggcat tggccagatt actggcggtt accgcctgtt
    32161 gattcagcgt ctggctggct gctcccatcg cggtataaat tgcgtgatcc attgagctat
    32221 cccgtcagcg attagcgtaa gttaaccagc gtattgagga tctggtcctg ggttttgatg
    32281 gtctgggcgt tagactgata gttacgctgg gcaacgatca tattgaccag ttctttactg
    32341 agatcgacgt tggacgcttc cagcgcaccg ttggtcagtg tgccaaagtt gcccgtcccg
    32401 gctgtcccca acagcgccac gccagaagat tgcgtagcgg accagacgtt gtcgccttcg
    32461 gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgggtt
    32521 tgttcgttgg aatagttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga
    32581 tcgcccggtt tgtagccgtt ctgggtggtt gccacaatgt tgttagcgcc ggtattttgc
    32641 tgcatggagt tgaggaagct caggctaaac gtggcgggat ctgcgccgtt aattgcgcca
    32701 gtggaaatat tcgctgttgg atttgaggtc agaacgccat tggcattaaa caccagtgtt
    32761 gtcgctgtct tcgcaatgct gtttggatca ctactatcct gggtgtagac ctgccagtta
    32821 ttatccccgg tcttcacaaa gtacacgctc atatcatgag cattaccctg actatcgaaa
    32881 acagtcaccg aacctttttt gttatagcta tccgcattgc tggcatcaaa tgcgttaaca
    32941 gagggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt ggtagttttc
    33001 gctgccatca gggtattcgg aatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    33061 ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac cttgcatatt caccaggtta
    33121 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc attgctgtct
    33181 accagacgga aaaaaccgtt ctggctaata gcaacgtcca gaccacgccc ggtgttggtg
    33241 gtcgtgccat cggtaaagtc ctgagtgata ccagcaactt ttacccccag tcccactttc
    33301 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag
    33361 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    33421 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    33481 tcgtcgaggg tagtggtgcc gtaagtgccg agatccagcg tattaccgtt gttgccgcgg
    33541 atcaccccct gcaccagagc aaactgcagc ggttgggcaa ccagttgtgt accaccgtta
    33601 ctggcgctaa tcgctacatt gtaagaacca ttcgtcgcag tgctgccgtc agtcaacgta
    33661 ccgtcccagg tgaaactgtg gactccggca gtcagttcac cgatatcaat ggtacggacg
    33721 accgcgccgt ttttatcggt gatggtggcc gtcactttgt ctgctgcctg ttgcaactca
    33781 acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccgattcc cgcaagaaca
    33841 gtggtaccgg ggatcatcac gccgtgacca atcaggttac tggcctgtaa cgactggctg
    33901 ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat cccactgacc
    33961 gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    34021 tggtttttca gctgcgccac caacaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    34081 ctgcccgtga gcgaactact gctggtggta ctgacgccgg tattcgtcgg atcggtggtg
    34141 gttaccgcaa tagacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca
    34201 tgcttttcac cgtgttgagc acttcaacat tggcctgata actgcgtgac gccgacatgg
    34261 tgttaaccat ctctccgacg acatcaacgt ttggcatttt cacgtagccc tttgcatccg
    34321 ccagcggatt acccggttca taaaccagtt tgtccggagc ctgactttct ataacatcgg
    34381 caacctttac gccgcctgtc gcagcacctg gcgcagcgtt aacctggaat accacctgtt
    34441 ttgcccgata tggctgtcca tcgggaccgg tcacgctatc agcattcgcc agattactgg
    34501 ccgccacgtt caggcgctgg gactgggcag ttaacgccga cccggcgata tcaaaaatat
    34561 tcagcagtgc catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatctgcc
    34621 cgctcaacgc gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt
    34681 cgcgatccat atcgacagta ttcccgtcaa gcgaaggctg gtccggaata cggtattgca
    34741 gttctgcgga aggaggcgtc agcgcctgcg ccggaatgtg tttcgttgag gtcatcgtca
    34801 gagcaaccac actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa
    34861 aatcgatatc gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca
    34921 cttcctgacg ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga
    34981 gcttatcgag catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt
    35041 acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc
    35101 attgcggaca ggtacaattc acgttgtaga aatggctggg ggcgaaaatg ctgacaataa
    35161 aacgtagcgt ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg agcaatctca
    35221 catcgcaatt gcacaccttt tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg
    35281 tttctattcg tacagcaccc aatctgctac cgccgtgcga tcagccattg ctttcgatga
    35341 gcaataattc ccgtctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac
    35401 gatatttaca ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcccattg
    35461 tgcgtggagg aaagctggaa gctggcaatg tcaaactgaa acgcggacgg ctggatacct
    35521 tgccaccgcg tacggtgctg gatatcaatc aacttgttga tgccgttagc ctgcgcgatc
    35581 tatcacccga tcaacctatc cagttaaccc agtttcgcca ggcatggcgg ataaaagcgg
    35641 ggcaacgcgt caacgttatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg
    35701 cgctgaacaa tgcagccgtc gcacaaaatg cgcgggtgcg catggtatcg ggacaagtgg
    35761 tcagcggcgt tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt
    35821 acccgtccct tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa
    35881 ccatgagtat tgatcgcact tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa
    35941 ccactgacgc gccggtaaca aacacccggg cggcaaaaac aacagcctcc accagcacca
    36001 gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc
    36061 ttgaacgtgt cgaagcgtta aagctggcga ttcgtaacgg tgaactaaaa atggacaccg
    36121 gcaaaattgc cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aattgatcgt
    36181 atgacgcgtc ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg
    36241 gtaatggatc aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa
    36301 tggattacag aacaaaaaag ctctctgctg gcgacgctgg attacctcga acagttacgc
    36361 aggaaagaac ccaatacagc aaatagcgtt gatattagtc aacgctggca ggaaattact
    36421 ggcaaaacgc agcaactacg ccaactgaat caacataacg gctggttact ggaaggacag
    36481 attgaacgca atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctgtat
    36541 ggggcgaacg gtcagacctc aacaacccat cgcggcagta aaaagatttc gatctgaaga
    36601 ttaactccgg cctgcactgc aggccggaat gtatcgttac accgtccggc gggcaaattc
    36661 tttaactttg aagcccagta ctgccagtgc agcgaagtac gcggcaatcc ccgccagcac
    36721 gaccgccatt aaacgcagta aacgccaggg catagtaccc aatgaccact ccggcatgat
    36781 atgtaacata cctaaaagca cgccagacat caccagtacc gccaccacca gacgcaacag
    36841 aaacgccatc cagccgggtt gcggggtaaa gattttctgc ttacgcaact gccagtaaag
    36901 cagcgaagca ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa
    36961 cggaccaata aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt
    37021 cactggcgtt ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat
    37081 caggccaatc aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa
    37141 ggcggtaaat ttaccgtact ggaacagcga aacggttaac ggaccggaaa gaatgcccaa
    37201 cgcaaccgca cttggcagcg ccaacaggaa acaaagacgc agcccccagt ccatcaaacg
    37261 gttgtattca tcatgattgc cactggcaaa acttttcgac agcgacggta gcaaaatggt
    37321 gccaagcgcc acgcccagca caccggacgg aaactccatt aagcggtcgg cgtaatacat
    37381 ccacgacacc gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat
    37441 ctggctcaca gagacgccaa ggattgccgg tcccatctgt ttcaccacac gcattgctcc
    37501 ggcatcgtgg aagttaatgc gtggcaaaac cagcatgccg atcttcttca gatgaggtag
    37561 ctgatacacc agctgcagga tgccgccgac cgtaacagcc caggccagcg ccagcaccgg
    37621 tggattaaag tacggcgcgg caaacaacgc aaaaccaatc atgctgatat tgagcaatgt
    37681 cggggcaaac gccggaattg agaagcggtt ccaggtattc agaatcgctc ccaccagcga
    37741 cgccagggag atcagcaaga tatagggaaa ggttatcttt agtagctggc tggtcagggc
    37801 aaatttgtcg gcagtgtcag cgaagccagg cgcggtcacc atgatcaccc acggtgcagc
    37861 gagcatgcca gcgaccgtca caaccgccag cgcaagtgtc agcaggccag aaacataaga
    37921 gacaaagacc cgcgtggcgt cttcaccctg cttgctttta tattctgcca gaatcggtac
    37981 aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt aacaagttag gaagtttaaa
    38041 agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc
    38101 tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt
    38161 taataaattc atcggtgttc taatccagac ccacgggcaa aacgcctgca aggcaggcgc
    38221 tgaaagaggc gaatagtcta cctggattat ggtgaattgc taccgccaga tgttacaggg
    38281 tttattcact catcgcatcg cgccagatct tgtcaacgat acgttgcgcc agcacggcct
    38341 gttcgccggc ggtttgcgga actgtctgat tttgcacgca ttcaatgaag tgccgcgcac
    38401 agccgacaaa tccacgttgc tcgagcgtac tctgccaacc aggaatcggt ttatgcacta
    38461 cgccctgccc gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccat
    38521 cagtcacggc ctgcacggtt tcacgctgac ttccggcccg gcgatgcata caggtggtga
    38581 tctgcaaagg accagccaaa aaatggtgct cggcaaacag catttcgccg gactcattgg
    38641 tcagcaacgt accgctttcg agagttgcat tgccgccaga caaccacagc gcggtatcca
    38701 ccacatgcag ataatcatcc agcaatgtga aataaagatc gtgtggcccg acgctattgg
    38761 tacgatgttt atccattctt agcgaggacg cggtggcgag ttgcgttttt aactcaccgt
    38821 agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc cgcgccgcca
    38881 gttccaccag ccgttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat
    38941 gtacccccgc attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggaatgca
    39001 caaaaaccgc atcgcaactg gcggcaaggc tggataacga atcagcataa ggaatgcgcc
    39061 agctgtcaca aattggcaag gctttggcgc gcgtaggcga ccacgcacct tgtaacgtcc
    39121 agtcagacgc tgccgccagc accggtaacc acgctttttg cgcgatgccg cctaacccca
    39181 ctacgccgat acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga
    39241 cgctgtttca gttctgccac ttcgatttcc agggcttcga cacgggcctg taaatcaccg
    39301 tcaaccgcgt tcaacatatc cgttaccgcc ggcggattct caacctcacc actgaaaaga
    39361 tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca
    39421 tcttcgcgac tcgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca
    39481 tacattcgcg cggcgcggct gcgcagttcc cccggcgtct gggcaccacg caataacaac
    39541 gtggtgatta acgccacttc tgcggcgctc agtttcagat cgccaaattc tgaattacaa
    39601 aaacgttgct catatttggt gacccgatta ccaaaaccgc tcaccgtgcg tagataatga
    39661 cgtttgacca gattatccag ctgttcctgc acttcagatt cactcagatt cataaccggt
    39721 tcacggttcg ttttctgatt acaggccgtg actacaccat ttacggagag cgggtattgc
    39781 tccggcgtgg tcacctgttt ttccagcaag caaccaatca cgcgagcttc catggcagta
    39841 agttgatatt tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc
    39901 cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc
    39961 tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt
    40021 agccataatg cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc
    40081 ttcaaacatg agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc
    40141 atgaaaagag ccgcgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc
    40201 cgggtcgaat aagccaaagt aaaaagctga accttgttta tgaaattcgc taatcatccc
    40261 tagcctggcc tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg
    40321 cttgaggaaa tggcgattct ctgcgtaata atccgccaga cgccaggcat cacgatcatg
    40381 caccagacgc acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata
    40441 gccaaacatt ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta
    40501 tacctgcgca accagaccct gtgaaaagtc cagaatgcca ttttctgcat tattcacata
    40561 tttgataaaa actctctatc aaagcgacgt tttgataaaa aaatattgtc cccgaagtga
    40621 tgggaaaagt cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg
    40681 ggaaatgtcc cgcgtgtcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa
    40741 tatgctggtc gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga
    40801 tcaaatgggc tgggccgccg tcatggtcgg tattgccctc ggtctacgcc agtttattca
    40861 gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat
    40921 tgtcaccggt atgctgatgc gtgccgccgg attcgccaca atgggtatcg cccacgaacc
    40981 gtggctactg tggttttcat gcctgctttc gggactcggc ggcacgttgt ttgatcctcc
    41041 gcgttcggcg ctggtggtga aactaatccg tccacaacag cgtggtcgtt ttttctcgct
    41101 gttgatgatg caggacagtg ccggtgcggt cattggcgca ttgttgggga gctggctgtt
    41161 gcaatacgac tttcgcctgg tctgcgccac aggggcagtt ttgtttgtgc tgtgtgcagc
    41221 gttcaatgcg tggttgttac cggcatggaa actctccacc gtacgtacgc ccgttcgcga
    41281 aggcatgacc cgcgtgatgc gtgacaagtg ttttgtcacc tatgtcctga cgctggcggg
    41341 ttactacatg ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc
    41401 gggcgcgccc tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt
    41461 gctctaccct atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttaatggc
    41521 tgggctgttg ataatgtcat taagcatgat gccagtaggc atggtcagcg gcctgcaaca
    41581 acttttcacc ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga
    41641 aaccttaagt gcttcgctgg cagacgcaag agctcgcggc agctatatgg ggtttagccg
    41701 tctgggtctg gcgattggcg gcgctattgg ttatatcggt ggcggctggc tgtttgacct
    41761 gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg ctgggcatta ttggcatctt
    41821 cactttcctt gcgctgggtt ggcagtttag ccagaaacgc gccgcgcgtc gtttgcttga
    41881 acgcgacgcc tgatttgata ctcccccctg actcctccat actgaaagag aactgacagc
    41941 caaatggagg agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc
    42001 ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga
    42061 cgcagaaaca cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga
    42121 cagccgctat atgccagaaa gcatggacat cgttcactat gtcgataaac tcgacggcaa
    42181 accgttactg accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg
    42241 ctacgccaac aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac
    42301 tcctgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta attttgccga
    42361 cctgctggcc cactctgacg gtctgattaa gaatatcagc gatgatttac gtgcgctgga
    42421 caaactgatc gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct
    42481 atttccgctc ctgcgtaatc tgacgctagt agccggaatc aactggccaa gccgcgttgc
    42541 tgattaccgc gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat
    42601 ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc
    42661 tgctggcgcg tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt
    42721 tcatgtcgag gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc
    42781 tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt
    42841 cgcttgcgaa acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc
    42901 atattgttct gacaaacctg accagccaaa ttggtcgcga agagccgaat aaggttaccc
    42961 taaccggaga cgccaatctg gacatgaact ccctgttcgg tagccagaaa gcgaccatga
    43021 aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag
    43081 agatggaagt ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt
    43141 tgctccccta tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc
    43201 tgcgcgaaga tggcagccag ggcgaagcaa tggcgaagaa actggcgaaa ggcattgaag
    43261 tgaagccagg cgaaattgtc attccgttta cggattaatc acgagggcgc attcgcgccc
    43321 tttatttttc gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc
    43381 cttcagccgg agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc
    43441 cagacgactg gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata
    43501 ccagcgaaat ttatggacgg gctatcgtaa tgcccaatct ggctccgccc gtgaccaccg
    43561 ttgaggctgc cgtggcgtat cgccagcgca ttcttgacgc cgtacctgcc gggcacgatt
    43621 tcaccccgct gatgacctgt tatttaacag attcgctgga ccctaatgag ctggagcgcg
    43681 gatttaacga aggcgtgttc accgctgcaa aactttatcc ggcaaacgca accactaact
    43741 ccagccacgg cgttacgtca gttgacgcaa tcatgccggt acttgagcgc atggaaaaaa
    43801 tcggtatgcc gctactggtg catggtgaag taacacatgc agatatcgac atttttgatc
    43861 gtgaagcgcg ctttatagaa agcgtgatgg aaccgctgcg ccagcgcctg accgcgctga
    43921 aagtcgtttt tgagcatatc accaccaaag acgctgccga ctatgtccgt gacggaaatg
    43981 aacggctggc tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg
    44041 ttggtggcgt gcgtccgcac ctgtattgtc tacccatcct caaacgcaat atccaccaac
    44101 aggcattgcg tgaactggtc gccagcggtt ttaatcgtgt attcctcgga acggattctg
    44161 cgccacatgc acgtcatcgc aaagagagca gctgcggctg cgcgggctgt ttcaacgccc
    44221 caaccgcgct gggcagttac gctaccgtct ttgaagagat gaatgctttg cagcactttg
    44281 aagcattctg ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca
    44341 tcgaactggt acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacacgc
    44401 tggtgccatt cctcgccgga gaaacggtac gctggtccgt taaacaataa aaatcgtagc
    44461 ttcctgttgt cattaggtta ttttacctgt ataaataacc agtatattca acagggggct
    44521 attatgcgaa ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac
    44581 gccctggctg gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac
    44641 gtatcggtac gttatgccgc agcaaataat ttatcggtta ttggcgcgac aaaagaagat
    44701 aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc
    44761 agcgaataat atgcagtgat ttttgttttt gccgggtcgc cccggctttt ttattttact
    44821 tgcgaattgt gagtacaaaa tttctacact tcctcaaaat aattgcacaa tatgctaaaa
    44881 aaatatgaat gacatgctgt tttattgttc at
//
LOCUS       DAZFRG010000006        43527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFRG010000006 DAZFRG010000000
VERSION     DAZFRG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.006"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            200..1771
                     /gene="garD"
                     /locus_tag="ACHKUC_000219"
     CDS             200..1771
                     /gene="garD"
                     /locus_tag="ACHKUC_000219"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO6616552.1"
                     /translation="MANIKIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTNDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1920..2255
                     /gene="prlF"
                     /locus_tag="ACHKUC_000220"
     CDS             1920..2255
                     /gene="prlF"
                     /locus_tag="ACHKUC_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO6616553.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2255..2719
                     /gene="yhaV"
                     /locus_tag="ACHKUC_000221"
     CDS             2255..2719
                     /gene="yhaV"
                     /locus_tag="ACHKUC_000221"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO6616554.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2774..3583)
                     /gene="agaR"
                     /locus_tag="ACHKUC_000222"
     CDS             complement(2774..3583)
                     /gene="agaR"
                     /locus_tag="ACHKUC_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO6616555.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3832..5112
                     /gene="kbaZ"
                     /locus_tag="ACHKUC_000223"
     CDS             3832..5112
                     /gene="kbaZ"
                     /locus_tag="ACHKUC_000223"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO6616556.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKAAALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5135..5608
                     /gene="agaV"
                     /locus_tag="ACHKUC_000224"
     CDS             5135..5608
                     /gene="agaV"
                     /locus_tag="ACHKUC_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO6616557.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5619..6398
                     /gene="agaW"
                     /locus_tag="ACHKUC_000225"
     CDS             5619..6398
                     /gene="agaW"
                     /locus_tag="ACHKUC_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO6616558.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6388..7266
                     /gene="agaE"
                     /locus_tag="ACHKUC_000226"
     CDS             6388..7266
                     /gene="agaE"
                     /locus_tag="ACHKUC_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO6616559.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AITVVLGIVGKFCHFL"
     gene            7284..7718
                     /gene="agaF"
                     /locus_tag="ACHKUC_000227"
     CDS             7284..7718
                     /gene="agaF"
                     /locus_tag="ACHKUC_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO6616560.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7715..8848
                     /gene="nagA"
                     /locus_tag="ACHKUC_000228"
     CDS             7715..8848
                     /gene="nagA"
                     /locus_tag="ACHKUC_000228"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6616561.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9198..10352
                     /gene="agaS"
                     /locus_tag="ACHKUC_000229"
     CDS             9198..10352
                     /gene="agaS"
                     /locus_tag="ACHKUC_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO6616562.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNSF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQSAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10365..11225
                     /gene="kbaY"
                     /locus_tag="ACHKUC_000230"
     CDS             10365..11225
                     /gene="kbaY"
                     /locus_tag="ACHKUC_000230"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO6616563.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSMTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11392..11868
                     /gene="agaB"
                     /locus_tag="ACHKUC_000231"
     CDS             11392..11868
                     /gene="agaB"
                     /locus_tag="ACHKUC_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO6616564.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11907..12710
                     /gene="agaC"
                     /locus_tag="ACHKUC_000232"
     CDS             11907..12710
                     /gene="agaC"
                     /locus_tag="ACHKUC_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO6616565.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12700..13491
                     /gene="agaD"
                     /locus_tag="ACHKUC_000233"
     CDS             12700..13491
                     /gene="agaD"
                     /locus_tag="ACHKUC_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO6616566.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13492..14259
                     /gene="agaI"
                     /locus_tag="ACHKUC_000234"
     CDS             13492..14259
                     /gene="agaI"
                     /locus_tag="ACHKUC_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO6616567.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQIENYTALSERASEYLLAVIR
                     RKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(14309..15172)
                     /gene="rsmI"
                     /locus_tag="ACHKUC_000235"
     CDS             complement(14309..15172)
                     /gene="rsmI"
                     /locus_tag="ACHKUC_000235"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO6616568.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15237..17273
                     /gene="lpoA"
                     /locus_tag="ACHKUC_000236"
     CDS             15237..17273
                     /gene="lpoA"
                     /locus_tag="ACHKUC_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO6616569.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQSQPQTVDDVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGPVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGSRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17231..17626
                     /gene="yraN"
                     /locus_tag="ACHKUC_000237"
     CDS             17231..17626
                     /gene="yraN"
                     /locus_tag="ACHKUC_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO6616570.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17646..18236
                     /gene="diaA"
                     /locus_tag="ACHKUC_000238"
     CDS             17646..18236
                     /gene="diaA"
                     /locus_tag="ACHKUC_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO6616571.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18246..18821
                     /gene="dolP"
                     /locus_tag="ACHKUC_000239"
     CDS             18246..18821
                     /gene="dolP"
                     /locus_tag="ACHKUC_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6616572.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18934..19974)
                     /gene="yraQ"
                     /locus_tag="ACHKUC_000240"
     CDS             complement(18934..19974)
                     /gene="yraQ"
                     /locus_tag="ACHKUC_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6616573.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMVYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(20047..20682)
                     /gene="yraR"
                     /locus_tag="ACHKUC_000241"
     CDS             complement(20047..20682)
                     /gene="yraR"
                     /locus_tag="ACHKUC_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6616574.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20810..21328
                     /gene="yhbO"
                     /locus_tag="ACHKUC_000242"
     CDS             20810..21328
                     /gene="yhbO"
                     /locus_tag="ACHKUC_000242"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO6616575.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21308..21751)
                     /gene="yhbP"
                     /locus_tag="ACHKUC_000243"
     CDS             complement(21308..21751)
                     /gene="yhbP"
                     /locus_tag="ACHKUC_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO6616576.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21802..22104
                     /gene="yhbQ"
                     /locus_tag="ACHKUC_000244"
     CDS             21802..22104
                     /gene="yhbQ"
                     /locus_tag="ACHKUC_000244"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO6616577.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAECAVFAELLNSLQTPEIKSD"
     gene            complement(22091..22594)
                     /gene="yhbS"
                     /locus_tag="ACHKUC_000245"
     CDS             complement(22091..22594)
                     /gene="yhbS"
                     /locus_tag="ACHKUC_000245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6616578.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22588..23112)
                     /gene="ubiT"
                     /locus_tag="ACHKUC_000246"
     CDS             complement(22588..23112)
                     /gene="ubiT"
                     /locus_tag="ACHKUC_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO6616579.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23321..24316
                     /gene="ubiU"
                     /locus_tag="ACHKUC_000247"
     CDS             23321..24316
                     /gene="ubiU"
                     /locus_tag="ACHKUC_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO6616580.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHHRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24325..25203
                     /gene="ubiV"
                     /locus_tag="ACHKUC_000248"
     CDS             24325..25203
                     /gene="ubiV"
                     /locus_tag="ACHKUC_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO6616581.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25284..26291
                     /gene="yhbW"
                     /locus_tag="ACHKUC_000249"
     CDS             25284..26291
                     /gene="yhbW"
                     /locus_tag="ACHKUC_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO6616582.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKDELL
                     G"
     gene            complement(26409..27653)
                     /gene="mtr"
                     /locus_tag="ACHKUC_000250"
     CDS             complement(26409..27653)
                     /gene="mtr"
                     /locus_tag="ACHKUC_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO6616583.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(27807..29696)
                     /gene="deaD"
                     /locus_tag="ACHKUC_000251"
     CDS             complement(27807..29696)
                     /gene="deaD"
                     /locus_tag="ACHKUC_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO6616584.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(29689..29769)
                     /gene="yrbN"
                     /locus_tag="ACHKUC_000252"
     CDS             complement(29689..29769)
                     /gene="yrbN"
                     /locus_tag="ACHKUC_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO6616585.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(29876..30760)
                     /gene="nlpI"
                     /locus_tag="ACHKUC_000253"
     CDS             complement(29876..30760)
                     /gene="nlpI"
                     /locus_tag="ACHKUC_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO6616586.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(30869..33004)
                     /gene="pnp"
                     /locus_tag="ACHKUC_000254"
     CDS             complement(30869..33004)
                     /gene="pnp"
                     /locus_tag="ACHKUC_000254"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO6616587.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATGGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(33251..33520)
                     /gene="rpsO"
                     /locus_tag="ACHKUC_000255"
     CDS             complement(33251..33520)
                     /gene="rpsO"
                     /locus_tag="ACHKUC_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO6616588.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(33669..34613)
                     /gene="truB"
                     /locus_tag="ACHKUC_000256"
     CDS             complement(33669..34613)
                     /gene="truB"
                     /locus_tag="ACHKUC_000256"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO6616589.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(34613..35014)
                     /gene="rbfA"
                     /locus_tag="ACHKUC_000257"
     CDS             complement(34613..35014)
                     /gene="rbfA"
                     /locus_tag="ACHKUC_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO6616590.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(35178..37871)
                     /gene="infB"
                     /locus_tag="ACHKUC_000258"
     CDS             complement(35178..37871)
                     /gene="infB"
                     /locus_tag="ACHKUC_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO6616591.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRVAEEARRMAE
                     ENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHA
                     ESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAV
                     KGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGA
                     AAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFL
                     DTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKID
                     KPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKA
                     VRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEV
                     LEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLE
                     NMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDA
                     TLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELK
                     QQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFK
                     DDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(37896..39383)
                     /gene="nusA"
                     /locus_tag="ACHKUC_000259"
     CDS             complement(37896..39383)
                     /gene="nusA"
                     /locus_tag="ACHKUC_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO6616592.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(39411..39863)
                     /gene="rimP"
                     /locus_tag="ACHKUC_000260"
     CDS             complement(39411..39863)
                     /gene="rimP"
                     /locus_tag="ACHKUC_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO6616593.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(40070..40146)
                     /locus_tag="ACHKUC_000261"
     tRNA            complement(40070..40146)
                     /locus_tag="ACHKUC_000261"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40110..40112),aa:Met,seq:cat)
     gene            40494..41837
                     /gene="argG"
                     /locus_tag="ACHKUC_000262"
     CDS             40494..41837
                     /gene="argG"
                     /locus_tag="ACHKUC_000262"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO6616594.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(41845..43470)
                     /gene="yhbX"
                     /locus_tag="ACHKUC_000263"
     CDS             complement(41845..43470)
                     /gene="yhbX"
                     /locus_tag="ACHKUC_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6616595.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLIAVPFVLLIAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
BASE COUNT        10540 a        11613 c        11004 g        10370 t
ORIGIN      
        1 tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggaggtact
       61 gcaatccctg aaatgattga cattgatcac atttctgcat ttaaactcct gacattctta
      121 tttcacccaa tgaagttatt tatttttaaa tgagaccagg tcctcatttt aatagtccct
      181 ggctggagaa tattgcacaa tggccaacat caaaatcaga caagaaacgc caactgcgtt
      241 ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata atggcctgaa
      301 agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc aggggcataa
      361 agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg aggtgattgg
      421 ttacgccgtg cgcgcaatcc cacgcggaag ctggatcgac gaatcaatgg ttgtactacc
      481 ggaagcgccg ccgttacata cgctgccact ggcaaccaaa gtcccggaac ccttaccgcc
      541 gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa
      601 cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact acgtagtaaa
      661 aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg tggggctgaa
      721 tcatttgtac ggttgtggcg tggcgattaa cgcacccgcg gcagttgtac ctattcgtac
      781 cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga ttggtctggg
      841 ttgtgaaaag ttgcagcctg aacgcctact gactggaacg aatgatgtgc aagctattcc
      901 agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt ttcagtccat
      961 ggtcgaggat attttgcagg tcgccgaatg ccatctacaa aaactgaatc aacgtcagcg
     1021 agaaacctgt ccggcttcag aactggttgt cggcatgcag tgcggtggca gcgatgcgtt
     1081 ttctggcgta acggcaaacc cggcggttgg gtatgcgtct gatctattgg tgcgctgcgg
     1141 cgcaacggtg atgttttcag aagtcacaga agtgcgtgac gcgatacatt tgctgacacc
     1201 acgcgctgtg aacgaagagg tcggcaaacg gctgctggag gagatggagt ggtacgataa
     1261 ctatctcaat atgggaaaaa ccgatcgcag cgccaaccct tcgccgggga acaagaaagg
     1321 cggtctggca aatgtggtgg agaaggcact cggctccatt gctaaatcgg gtaaaagtgc
     1381 aattgttgaa gtgctgtcgc ccggccaacg cccgactaaa cgcggattaa tttacgccgc
     1441 gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca
     1501 agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg tcattaaaat
     1561 ggcgacccgc accgagctgg cgaaccgctg gtttgattta atggatatta acgcgggcac
     1621 tatcgctacc ggcgaagaaa ccattgaaga agtgggctgg aagttgtttc actttattct
     1681 cgacgtcgcc agcgggaaga agaaaacttt ctcggatcaa tggggactgc ataaccagct
     1741 ggcggtgttt aacccggcac cggtaacctg atttcttttg gaacgtccct cgcaaaacat
     1801 ggccttagtg ccatgttttt attgtttaaa gtccacacat ccattaataa tgcatttgca
     1861 ttaccttagt tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtaaataa
     1921 tgcccgctaa tgctcgctct aacgctgtac tgaccactga atcaaaggtc actatacgcg
     1981 gacaaacaac tatccccgcg ccagtgcgtg aggccttaaa actgaagcct ggcctggaca
     2041 gcattcatta cgaaattctg cctggtgggc aagtatttat gtgccgactg ggagatgaac
     2101 aggaggatca tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaacc
     2161 cgcaaaaaac tcgtccattc gacattcaac aaggaaagaa acttgtcgct ggcatggacg
     2221 tcaacattga cgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg
     2281 ttgggcgcta tatgctcatc cctgttttca ggaaacctac gatgctttag ttgccgaagt
     2341 cgaggcatta aagggaaaag atcctgaaaa ttatcagaga aaagctgcca caaagttatt
     2401 ggcggtggtc cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt
     2461 ccgtcatggc aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg
     2521 cgctggtcgc tatcgtctct tctttcgtta cagtgaaaaa gagaaagtca tcattctggg
     2581 atggatgaac gatgaaaaca cgctgcgcac ctacggtaaa aaaacagatg cctataccgt
     2641 attcagtaaa atgttaaaaa gaggacatcc tcctgccgac tgggaaagcc tcacccaaga
     2701 gacagaagaa aaccattgat ggtgttcaca atgcccgccg aacgcattgg tgttgaccag
     2761 cgggcatcct ttctcattcc ccgaccagaa tcacttcaac cccagccttt ctcagtcctt
     2821 ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa
     2881 tgatcttatg taaactagaa cgattgaact tactggaatc ggtgaccacg atgatccgtt
     2941 ccgcaacttc gcacatccga cggtttaaac gggcttcatc ttcattatgc gtgctgacgc
     3001 cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca
     3061 gcgattgctc agcctgatca ccgtaaaaag attgcgactg acggcgcaaa tgccccccgg
     3121 tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg
     3181 tcatcgcaat tacgtcagtg tgcttgcgca tcagacgagc aatctcaaaa gtggtggtcc
     3241 cggagtcgag gatcacccga tgaccaggct gaatcaactc aacggcagct ttcgcaacgc
     3301 tgcgtttcat cgcggtgttc agcgcgcttt tatcttccac tgatggctcg actgacggcg
     3361 ttgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca
     3421 gaaacgccag atcgttgcgg atcgtcacag tagatacacc atacaacgcc gatagatcat
     3481 taacttgcac acttccttgc tggcgcaggc gttgaatgat ctgctctcgt cgctcactgg
     3541 tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa
     3601 aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca
     3661 ccttacccat tggcgcaagc tactctcgtt tcagtggctt tcattatgtt tcttttgtga
     3721 atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa
     3781 ctgaaacaaa acgaaagatt aatgtcgcag taatctgaac tggagaggaa agtgaaacat
     3841 ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgggattta tgccgtttgt
     3901 tccgcacatc cgctggtgct ggaagctgca atccgctacg ccagtgcaaa ccaaacgccg
     3961 ttactgattg aagcaacctc caatcaggtg gaccagttcg gcggttatac cggaatgacg
     4021 cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat
     4081 gcgctgattc tgggtggcga ccatctgggg ccaaaccgct ggcaaaacct gcctgccgct
     4141 caggcaatgg ccaatgccga tgatttgatt aaaagctacg tggcggcagg attcaaaaaa
     4201 attcaccttg attgcagcat gtcctgtcag gacgatccga ttcccttaac tgatgacatc
     4261 gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg tgaacacttt
     4321 ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat
     4381 gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc
     4441 catcgtcacg cctttgaaaa gcaaggtctg aatgccatct ggccacgcat cattgccctg
     4501 gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca gccagccaaa
     4561 gccgctgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct
     4621 accgattatc aaacaccgca atcgctgcgc cagctggtga ttgaccactt tgccattctg
     4681 aaagttggcc cggcgctgac ctttgctctg cgtgaagctc tgttctctct ggcggcgatt
     4741 gaagaagaac tggtgccagc aaaagcctgt tctggtctgc gtcaggtgct ggaaaacgtg
     4801 atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt
     4861 ctggcgcgtg gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt
     4921 gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctgccgctg
     4981 atcagtcagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcagccaacg
     5041 ccacgggaac tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt
     5101 gaaggccaat aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagtcggat
     5161 tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa
     5221 tctggtgctg gtagccaacg atgaggttgc cgaagatctg gtacaacaaa acctgatgga
     5281 aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag tcatcgacaa
     5341 cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac cagccgattt
     5401 cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta
     5461 cgccaatggc aagcaacaaa tcgccaaaac ggtttctgtg gatgcgggtg atatcgcagc
     5521 atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc
     5581 tgctgtagac ctctttaaat tactttgagg gattcatcat ggaaatcagt ctgttgcagg
     5641 catttgcgtt gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcttaaccc
     5701 atatgcaccg cccggtggtt ctcggcccgt tggtcgggct ggtacttggc gatctgcata
     5761 ccggtatttt aaccggcggt acgctggaac tggtgtggat ggggctggcc cctctggcgg
     5821 gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta
     5881 ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga
     5941 tggggattac cttcctgttc tcggtgatgt ctggcgtaat gtcccgctgc gaccggatgg
     6001 cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcattg ctggcactcg
     6061 gcactttcta ttttctctgc gctttcctac ctatctactt cggcgcagaa catgccaaaa
     6121 ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca
     6181 tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct
     6241 acttcatcct gggctttgtt gccgcagcct ggctcaagtt accggtgttg gctattgctg
     6301 ccgctgcgct ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac
     6361 ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc
     6421 agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcagagc
     6481 attggcgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgttgtta
     6541 caggcttctt tcaactacga acgtatgcag gcttccggct ggctgtacgg cctgctgcca
     6601 gcactgaaaa agatccacac taataaacgc gacctggcgc gcgctatgaa ggggcatatg
     6661 ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg
     6721 gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg
     6781 ctcggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg tggcgggata
     6841 ggtgcaagcc tcgcgctaca aggctctatt cttggcgctg tcgtctttat tgtgctgttc
     6901 aacgtggtgc acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg
     6961 gcggcgattc cactcattaa agcaaatacc aaaaaagtcg gccatgcggc atctatcgtt
     7021 gggatgacgg taatcggcgc gctggtggca acctatgttc gtttaagcac cacgctggaa
     7081 atcaccgcgg gcgacgcagt ggttaagtta caggctgatg ttatcgacaa actgatgcca
     7141 gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg cggctggagt
     7201 ccgctgcgcc tgattgcgat taccgtggtt ctcggcatcg tcggtaaatt ctgccatttc
     7261 ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat ggcggatttg
     7321 ccagcggcat ggaaaaagca atgaagcaaa ttctcggtga gcaatcgcag tttatcgcca
     7381 tcgattttcc ggaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg
     7441 cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt
     7501 ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatc accggcacca
     7561 atttgcaact gctactggaa atggtgctgg agcgcgaagg gttaagcggt gaagagttcc
     7621 gtgtgcaggc gctggagtgt ggacaccgtg ggctgaccag tctggtggac gagttaggcc
     7681 gctgccatga agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag
     7741 gctgctgact gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtaat
     7801 cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgtcc
     7861 cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc
     7921 ggatgtgctc gacaagctgg caatgcacaa agcacgcgaa ggtgtcggca gttggttacc
     7981 gaccaccgta accgcgccgc ttaataccat tcatgcggcg ctggaacgta ttgctcaacg
     8041 ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt
     8101 cacgccgcag aataaaggcg cgcatccacc ggagctgttc cgcgagctgg aaattgccga
     8161 gctggatcaa ttgattgccg tttctcagca taccttgcgc gtggtggcgc tggcaccgga
     8221 aaaagaaggg gcattgcagg ccatccgcca tcttaaacag caaaacgtac gggtgatgct
     8281 ggggcatagc gcggcgacct ggcaacaaac ccgtgccgcg tttgatgctg gtgccgacgg
     8341 cctggtgcat tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg
     8401 cgcgggatta acagacaagc gcgcctggct ggaactgata gccgatggtc atcatgtaca
     8461 tccggcggca atgtcgctct gttgttgctg tgcgaaagag aggatcgtac tgatcaccga
     8521 cgcgatgcag gcagccggga tgccggatgg tcactatacg ttatgtggtg aagaagtgca
     8581 gatgcgcggt ggcgttgtcc gtaccgcgtc cggtgggctg gcgggcagta cgctgtctgt
     8641 cgatgcggca gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg aagccatcca
     8701 tatggcgtcg ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg gatcgcttaa
     8761 accgggcaaa cgcgccagcg tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg
     8821 gattcagggt caattagctt cgttttgatg gtttgctcct ttattaggcc ttcacttccc
     8881 ccgtaaggcc tttctttttc tttcgttttg atctgtgcag cggtgtcgga tgcgacgcta
     8941 atgcgtctta tccgacctac ggttggtgag cgtaaggccg gataaagtat ttacgccgca
     9001 tccagcaatc ccttttactt cccttacctt ttccttcaac gatcacagat ttcgttttat
     9061 ttcctttttc tccattgaac tttcagtttc ttttctatag attttaacca acgaaagaaa
     9121 tcaccaagtg aaatgaaacg aaaggcaagc gaaagcgaca acgccgacgt caagttcatc
     9181 agactaagga ttgagttatg ccagaaaatt acacccctgc tgctgccgca accggtacat
     9241 ggactgaaga agagatccgc catcagcctc gcgcatggat ccgttcactc accaacattg
     9301 acgcgctacg ttccgcgctc aatagcttcc ttgaaccgtt actgcgcaaa gagaatctgc
     9361 aaatcatcct gaccggagcc ggaacatcgg catttatcgg tgacatcatc gcgccgtggc
     9421 tcgccagcca taccggtaaa aacttcagcg ccgtaccgac caccgatctg gtcaccaatc
     9481 caatggacta cctgaaccca gctcatccac tactgttgat ctccttcggt cgatccggca
     9541 acagcccgga aagcgtcgcc gccgtggaac tggcaaatca atttgtacca gaatgctatc
     9601 acctgccgat cacctgcaac gaagcgggcg ctctttacca aaacgcgatc aacagcgaca
     9661 acgcgtttgc cctgctgatg cccgcagaaa cgcacgatcg cggcttcgcg atgaccagca
     9721 gcattaccac catgatggcc agctgcctcg cggttttcgc acctgagatg atcaacagcc
     9781 aaaccttccg cgacgtggcg gatcgttgcc aggcgatcct gacctcactg ggcgatttca
     9841 gcgaaggtgt gtttggttac gcaccgtgga aacggatcgt ttatctcggc agcggtggct
     9901 tacagagcgc agcacgcgag tcggcgctga aagtgctgga actgacagcg ggtaaactgg
     9961 cggccttcta tgattccccg accggattcc gtcatggacc gaaatcgctg gtcgataacg
    10021 aaacgctggt ggtggtgttt gtctccagcc acccttacac ccgtcagtat gatcttgatc
    10081 tgctggcaga actccgccgc gataaccagg caatgcgcgt aatcgccatc gccgcggaaa
    10141 gcaacgacgt tattaccgcc ggtccacata tcatcctgcc gccgtcccgt cactttatcg
    10201 acgttgagca ggcattttgc ttcctgatgt acgcccagac atttgcactg atgcagtcgc
    10261 tgcacatggg taatacgccg gataccccat cagccagtgg caccgttaac cgcgtggtgc
    10321 aaggcgtaat cattcatccg tggcaggcat aagaggatcg cattatgagc attatctcca
    10381 ccaaatatct gttacaggac gcccaggcca atggctacgc ggtgcctgcc tttaacattc
    10441 ataacgccga gacgatccag gcgatcctcg aagtgtgcag tgaaatgcga tcgccggtga
    10501 tcctcgccgg gacgccgggg acctttaagc atatcgcgct ggaagagatc tacgccctgt
    10561 gtagcgccta ttccatgacc tataacatgc cactggcact gcatcttgat caccacgaat
    10621 cgctggatga tattcgccgt aaagtccacg caggcgtgcg cagcgcgatg atcgacggca
    10681 gtcatttccc gtttgccgag aacgtgaagc tggtgaaatc ggttgtcgac ttctgccact
    10741 cccaggattg cagcgtggaa gcagaactgg gccgcctcgg cggtgttgaa gatgacatga
    10801 gcgttgacgc cgaaagtgca ttcctgaccg atccacaaga agctaaacgc tttgtcgaac
    10861 tgactggcgt cgacagcctg gcggtagcga ttggtacggc gcacggctta tacagcaaaa
    10921 cgccgaagat tgatttccag cggctggcgg aaattcgtga agtggtcgat gttcctctgg
    10981 tgctgcatgg tgccagcgat gtcccggatg aatttgtccg tcgcactatt gaacttggcg
    11041 taacaaaagt gaacgttgcc acagaattaa aaatagcctt cgctggcgcg gttaaagcct
    11101 ggtttgcgga aaatccgcag ggtaatgatc ctcgttatta tatgcgcgtc ggaatggatg
    11161 cgatgaaaga agttgtcaga aataaaatta atgtctgtgg ttcagcgaat cgaatttcag
    11221 cataatcatt agttttttat gatttatccc aatgtacttc ccgattaata tcggggagtg
    11281 ccttaatggt aaaggagata actaaacctt aataaataaa tcagtacaat atcgcaacaa
    11341 taatatattt aaaaagatta tattattcaa ctttatggtg aggattacac aatgagcagt
    11401 ccaaatattc tcttaacccg tattgataac cgtctggttc acggtcaggt tggcgtgacc
    11461 tggacatcca ccatcggtgc gaatctgtta gtagtcgtgg atgatgttgt cgctaacgat
    11521 gatattcaac agaaattaat gggtattacc gcagaaacct acggctttgg cattcgtttc
    11581 tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccacatca gaagatcttc
    11641 ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggtat tgacctgaaa
    11701 gatgtcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag cagtaaagtt
    11761 tatgtcgatg accaggatct cacggactta cgttttatta aacaacgtgg cgtgaatgtt
    11821 ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat ctaaaatcgc
    11881 cttaatattg atttgaggta ataaaaatgc atgaaataac cctactccag ggattatccc
    11941 tggcggcgtt agttttcttt ctggggattg atttttggct ggaagcctta tttttattcc
    12001 gcccgataat cgtttgtacc cttacaggtg ctattctcgg tgatattcag actggcttaa
    12061 ttaccggtgg cctgacagag ttggctttcg ccggattaac ccctgcaggt ggtgttcagc
    12121 cgcccaaccc tattatggcg ggtctgatga ccactgtcat tgcatggtct acgggcgttg
    12181 atgccaaaac ggcaattggt cttggcctgc cgtttagttt gttaatgcag tacgtcattc
    12241 tgttcttcta ttccgctttc tcattattta tgaccaaagc cgataaatgc gcaaaagagg
    12301 cggatacggc agcgttttcc cgacttaact ggacaacgat gctcatcgtc gcttcagcgt
    12361 atgcggtgat tgctttcctc tgtacttacc tggcacaagg ggcgatgcag gcgctggtga
    12421 aagcgatgcc cgcctggctg acccacggct ttgaagtggc tggcggtatt ctgcctgctg
    12481 ttggttttgg cttgctgctg cgcgtgatgt tcaaagcaca atatatccct tacctgatcg
    12541 ccggtttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt gccgtactgg
    12601 gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa gcgcaaccgc
    12661 agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga aatcagtaaa
    12721 aaagatatca cccgtctggg ctttcgttcg tcactgctac aagcaagctt taactacgaa
    12781 aggatgcagg cgggcggttt tacctgggcg atgttgccga tcctgaaaaa gatttataag
    12841 gacgacaaac cgggcctaag cgcggcgatg aaagataacc tcgaatttat taatacccac
    12901 cctaatctgg tcggattcct gatggggtta ttaatttcga tggaagaaaa aggagaaaac
    12961 cgcgacacca ttaaaggcct caaagtggcg ctgtttggcc caatcgccgg gattggcgat
    13021 gcgattttct ggtttacctt gttgccaatt atggcgggga tttgctcatc gtttgccagt
    13081 caggggaacc tgttgggacc gattttgttt ttcgccgttt acctgcttat ctttttcctg
    13141 cgcgtcggct ggacccacgt cggttattca gtcggcgtga aggctatcga taaagtgcga
    13201 gagaactcgc agatgattgc ccgttcggca accatcctcg ggatcacggt aatcggcggg
    13261 ctgatcgctt cgtatgtgca tattaacgtg gtgacgtcgt ttgccatcga cagtacccac
    13321 agcgtcgcac tgcaacagga tttcttcgat aaagtcttcc cgaatatttt accgatggcc
    13381 tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt gctgttaatc
    13441 ggcgtgacgt ttgttctttc tattgtttgt tctgcattcg gcattttgta aatggaacga
    13501 ggcaccgcgt ctggcggtgc ctctttactt aaggaatttc atcctgtgca aacccttcaa
    13561 caaattgaaa actatacggc gttaagtgaa cgtgccagcg aatatttatt ggccgtgatc
    13621 cgtcgcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt actgacgtat
    13681 cattatctgg tagaaaaaat ccaccagcag caggttgatg tcagccagct caccttcgtg
    13741 aagctcgacg aatgggtgga tctgccatta acgatgcctg gtacctgcga aactttcctt
    13801 caacagcata tcgtgcagcc gctggggcta cgtgaagacc agctcatcag ctttcactcc
    13861 gaagagataa atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg caaaggcggt
    13921 ctggacttat gcgttctcgg attggggaaa aacggtcatc ttgggctgaa cgaaccggga
    13981 gaaagcctgc aaccggcctg ccatatcagt caacttgatg ccagaacaca gcaacatgag
    14041 atgttaaaaa ccgcgggccg tcccgtgact cgtgggatca ccttaggcct gaaggatatt
    14101 ctcaataccc gcgaagtttt gctactggtg actggcgaag gaaagcagga tgcgacagaa
    14161 cgttttctca cggctaaagt ctctaccgct atcccggctt cgtttttatg gctgcataac
    14221 catttcacat gcgtgattga tgaaatgtgt cgtcgttaac tcgcctggta aaggcgttgt
    14281 agcgctattc ctgctgcaac gcctgtaatc actctccctg ctgctccagt gcatacttat
    14341 acagcgcatt tttcttcacg ccgtgaattt ctgctgccag cgccgccgct tttttcagtg
    14401 gcagttctgc ctgcaacagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct
    14461 gtgctttatg gccttcgaca atcagtacca tttcgccttt gcgacggttt tcatcttctt
    14521 tgacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca
    14581 gctcacgcgc cagaacgacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc
    14641 tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg
    14701 cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttca taacaaaaac
    14761 gatcggatgg taaacccgcg gcgcttaacg cagtgatagc agcacacggc ccgggtagcg
    14821 gcaccacgcg aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa
    14881 ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc agcttcgcca
    14941 gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa
    15001 tcccaaaatg ttgcagcaat aaaccggtgt gacgcgtatc ctcggcggca atcagatcaa
    15061 cggcctgtaa tacctctaat gcacgctggg tgatatcagc cagattgccg attggcgtcg
    15121 gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt
    15181 ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga tacattatgg
    15241 taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc
    15301 tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca
    15361 cggcgcaggc tgattctgcc ttttacctgc aacagatgca gcaaagttct gatgatacca
    15421 ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa accggacagg
    15481 cggtggagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa
    15541 cactgctggc ggcagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc
    15601 tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcccgttac tggcaggcaa
    15661 aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg
    15721 aaccgctgct cggtgcgaaa gaaaagcagc agaatattga cgccacatgg caggcgctct
    15781 cctccatgac tcaggagcag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc
    15841 aaggctggct ggatttgcag cgcgtctggt ttgataaccg taacgatcct gacatgatga
    15901 aagctgggat cgccgactgg cagaaacgtt atccgaacaa cccgggcgcg aaaatgctgc
    15961 caacgcagtt ggttaacgta aaagcgttta aaccagcatc gaccaacaaa atcgccctgc
    16021 tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag
    16081 cggcgaaaaa tatcggcact cagccagtgg cggcccaggt agctgccgca cctgccgcag
    16141 acgtagcaga acaatctcag ccgcaaaccg tggatgacgt tgccagcccg gcacaagcct
    16201 cggttagcga tctgaccggt gaacagcctg cggcccagcc ggtgcctgta agcgccccgg
    16261 cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg aaaatctacg
    16321 atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta
    16381 ttgtggtcgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc aacactccgc
    16441 tgaacgtgct ggcactgaac cagccggaga acatcgaaaa ccgcgtcaat atttgttact
    16501 tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta
    16561 aacaagcgcc gctggtgctg atcccacgca gttcattggg cgatcgcgta gccaatgcgt
    16621 ttgcgcaaga gtggcagaaa ctgggcggcg gccccgttct gcaacaaaaa tttggttcca
    16681 ccagcgaatt acgcgcgggt gttaacggcg gttcgggtat cgctttaacg ggtagcccga
    16741 ttactcccag agaaacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca
    16801 cgccaaccga tgatcagttc accaataatg gcagtcgtgt cgatgcggtg tacattgtgg
    16861 caacgccggg tgaaatcgct tttatcaaac caatgatcgc catgcgtaac ggtagccaga
    16921 gcggtgcaac gctgtacgcc agctcccgca gtgcgcaagg gaccgctggc ccggatttcc
    16981 gcctggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctgc
    17041 cgttaatgca gcaggcgctc agcgcggtga acaacgatta ttcgctggct cgcatgtatg
    17101 cgatgggcgt cgatgcctgg tcgttggcaa atcatttctc acaaatgcgc caggttcagg
    17161 gttttgaaat caacggtaat accggaagcc tgacggcaaa cccggattgc gtgattaaca
    17221 ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa
    17281 acagaccggc gatgcgtggg aagcacaagc gcgtcgctgg ctggaaggca aaggactacg
    17341 gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg
    17401 ccggaccacc atttttatcg aggtgcgtta ccgccgctct gcgctttatg gcggcgcggc
    17461 agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc
    17521 gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg
    17581 gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga
    17641 ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg
    17701 cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc
    17761 tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt
    17821 ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac
    17881 taaatactga taatgttgtc ttaacggcaa ttgccaacga tcgcttacat gatgaagtgt
    17941 atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc
    18001 gtggcaacag ccgcgatatt gtaaaagcgg tagaagctgc cgttacgcgt gatatgacca
    18061 ttgtggcatt aaccggctat gacggcggtg aacttgcagg tttgttaggg ccacaggatg
    18121 tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg
    18181 taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat gtttaaggag
    18241 aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt
    18301 gtgttgccgc tgccgtagtg ggtactgctg ctgtgggtac caaagccgca actgacccac
    18361 gcagtgtcgg cacccaggta gacgatggta ccctggaagt gcgcgtgaac agcgcattgt
    18421 cgaaagacga acaaattaag aaagaagcgc gcattaatgt aacggcctat cagggcaaag
    18481 tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa cagattgcta
    18541 tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc
    18601 tgggcgaagc gtctaacgat acgtggatca ccaccaaagt acgttcgcag ctgttaacca
    18661 gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga
    18721 tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtaagcg
    18781 gcgtgaagcg ggtaactacg gcgtttacgt ttattaaata gcagcccttg taatgcctga
    18841 tgcgacgctt gcagcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc
    18901 gtttacgccg catccgacat gttttccctc aaatcaaacc aacagcgcca gcccgccgac
    18961 aatcacacca gacactgcca ccatcgcccc cgtcagccat aaggctttcg ccgggaacgc
    19021 tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca acaatgccag
    19081 cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc
    19141 cgtgggaatg acaaacaagc atcctgccac cgccatcgcc accacccaca tcaggctgtt
    19201 atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca acaccagtac
    19261 tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc tgccccagcg
    19321 gctaaaaaaa ccgccttgtg attccggtat gtcaatttcg accggtgcct gcgtttgcgg
    19381 tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagccccgc
    19441 caccagacga atcgccgcaa aaccccagcc gaggacgaac cccataaaca ccagcgtcgc
    19501 cgggtttaac aacggattcc ccatccagaa tgccagcgca ccgcccatcg acacctgttg
    19561 gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatac ccggcagcga
    19621 aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcagcaacca
    19681 gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca ccgccgcttt
    19741 ccataccgcg aggaaataga ccatcgcgta atccaacgcc gcctgccacg gattagcatc
    19801 cgcctgcgca aggatagatt taccgatact gtgagtttcg gcggcagtaa aggctttacc
    19861 gtagtaaggt tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc
    19921 gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt
    19981 ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg
    20041 gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca caccttcatg
    20101 ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt
    20161 ccagttaccg ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt
    20221 gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc
    20281 gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt
    20341 agcacccata gcactgacca ccagcatatg ctgcgcaccg agccgccgcc cggttaatgc
    20401 ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttactcc ccgcttctcg
    20461 ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct gcgccagcgc
    20521 gtcggtcagt tgcggatcat ggggattgaa cactcctggc atatctccca acggacgtcg
    20581 cgtcggcgcg gctatggcgt taactttcgg ttcgttaatc aacatccgca gcaggtgacc
    20641 gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc ctttacagaa
    20701 ttgtctgcct tgcgctccac ggctcatgca ccaggcttaa tagatcatga ggtaattatc
    20761 cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa
    20821 aatcgccgtt ttaatcactg atgaatttga ggattcagaa tttacttcac ccgcagacga
    20881 gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta aaacggtgaa
    20941 aggcaaaaaa ggagaagcca gcgtggccat cgataaatcc atcgatgaag tgacgcccgc
    21001 agagttcgat gccctgctgc taccgggcgg tcattcacca gattatctgc gtggtgacaa
    21061 ccgttttgtc accttcacac gcgattttgt gaacagcggc aaacctgttt tcgccatctg
    21121 tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgactgccgt
    21181 taaaccaatc attattgatg ttaaaaacgc gggcgcggaa ttttacgatc aggaagtggt
    21241 ggtcgataaa gatcagttgg ttaccagccg gacaccggac gatctgcccg cgtttaaccg
    21301 cgaggcgtta cgcctgctcg gtgcctgagt tacgtagcca aatcattttt ttaccaaagc
    21361 ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccatacc ggggccgaca
    21421 gcattctggc aaccggaaag cgacgattgt acgccttgcg tgcgaggtcg ctttcctcgc
    21481 cttccagtct gcggatctca cctttaaact gcacgccgcg aattaacgct accgttttcg
    21541 gctgaccgtt taccgttccg gcaattgccg cctgcggccc gctcatctgc gcatgacgcg
    21601 ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga taaaaggcat
    21661 tggcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca
    21721 gccaacggct gatggcggtg agtgtttcca ttgctgttct cttttatact gtgggcctga
    21781 ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac
    21841 aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc
    21901 ggcaaagggg cgaaagcact gcgcgggaag ggagaactaa cgctggcgtt ttccgcacca
    21961 gtgggcgatc gttcgctggc gttacgggca gaatatcgcg ttaagcaact gaccaaacgg
    22021 caaaaagagc gtctggtagc agaatgtgca gtgtttgcag aactgttgaa cagcctgcaa
    22081 accccggaga ttaaaagcga ttgaagtgct cgtgatactc aaccagacca gttacaccgt
    22141 tcagcgcgtc atccgctaaa cgatgtacct ggaaggcact ttcagtgccc ggccagcggc
    22201 aacgcagatc gtgatgtgcc gccagttcaa agccgaaacg gctgtacagc gccggatcgc
    22261 ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca
    22321 gttggcgtgc cagcccctgt ccacggtatt tttcatctac cgccaacggt gccatgccga
    22381 cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct
    22441 gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt
    22501 gaaccagctt cgcttccgca tcactttcga atgaacgacg cagcagggca tcaatacccg
    22561 gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt
    22621 ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg
    22681 taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacagccc
    22741 cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg
    22801 tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg
    22861 cgcgttctgg ctaacgacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat
    22921 atcacgcaca tgaatactta accagcgacc ttcaagaaac tccagctcgc catcatccag
    22981 cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg
    23041 cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag
    23101 tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa
    23161 aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa
    23221 ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta
    23281 acatttttgc gttttgataa cgcaaccttc aggaaaaatt atggagctgc tctgccctgc
    23341 cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg
    23401 gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt
    23461 gcaggaagcg gtgagttttg tccatcaaca tcaccgcaaa cttcacatcg cgattaacac
    23521 ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg cggcgcagct
    23581 gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta
    23641 tccgcatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag aggcgattaa
    23701 cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt tgtcgattca
    23761 tcaggtgaaa caactggcac gggtcacacc tgtaccactg gaagtctttg ctttcggcag
    23821 cctgtgcatt atgtcggaag gtcgttgcta tctgtcgtcg tatttgacgg gtgaatcacc
    23881 caacaccgtg ggtgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg
    23941 gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg
    24001 ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga
    24061 agaaccaacc agtctcaata cccttgagct gctgccggag ttaatggcgg cgaatatcgc
    24121 ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg tggcgaaagt
    24181 ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtgc cgcaaagcgc
    24241 gtggatggag acgctcgggt cgatgtccga aggcacgcaa actacccttg gcgcgtatca
    24301 ccgtaaatgg cagtgagaaa agcaatgaaa tattccttag ggccggtgct atggtactgg
    24361 ccaaaagaga cgctggaaga attttatcag caggccgccg ccagcagcgc agacgtgatt
    24421 tatcttggtg aagcggtctg tagcaaacgc cgggcaacca aagttggcga ctggctggag
    24481 atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg
    24541 caggcatcct ccgaactggg cgaactgaaa cgctatgttg agaacggtga gtttctgatt
    24601 gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc
    24661 gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg
    24721 atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag
    24781 tgcgatgagt tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta
    24841 cctctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag
    24901 tgtgaaacct gctgcattaa atatccgaac gggcgcaacg tactgtcgca ggaaaaccaa
    24961 caagtgtttg tactcaacgg cattcagacc atgagcggct acgtttacaa cctcggtaac
    25021 gagctggcat ccatgcaggg gctggtcgat gtggtccgcc tgtcaccgca gggtaccgac
    25081 actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgcgcc actcccgttg
    25141 acggcaaaca gcgactgtaa cggctactgg cggcggctgg ccgggctgga gctgcaagcg
    25201 taagtatata gctcactttg ttaacaactt taactactct ttaatgcagt attaaagact
    25261 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc
    25321 tggcccccat ccccgaaggt tcttcagcgc gggaagcgtt ctcccactct ctcgacctcg
    25381 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    25441 ccggcattgc cagtgctgca acgtcggtgt taattggcta tctggcggcg aataccacca
    25501 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccattg gtgatcgccg
    25561 aacagttcgg cacgctcaat acgctctatc cggggcgaat cgatttgggg ctgggtcgtg
    25621 caccgggtag cgatcagcga accatgatgg cgctgcgtcg tcatatgagc ggcgatattg
    25681 ataatttccc ccgcgatgtc gcggaactgg tggactggtt tgacgcccgc gatcccaatc
    25741 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    25801 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt gcctcacact
    25861 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    25921 cacggttgga gaaaccgtat gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    25981 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    26041 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    26101 agtatggtgt gcagcaggcg ctgagtatgt cgctggtggg cgataaagcg aaagtgcgtc
    26161 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtt aacgggcaga
    26221 ttttcgacca ccaggcacgc ctgcattcat ttgagctggc gatggatgtg aaggacgagt
    26281 tgttggggta gtgtgtttta acgtaggaag ccttatccga gatggcaacg ctgtcctacg
    26341 taggcctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    26401 accgctgatt actgatacac cggcagcaca ttaaagctcg ataagatatg gaccagtgcg
    26461 ttgccaacgc caaataccag aatcagcata atcatcggct tgccgcccca gacacggaat
    26521 ttcgggctac caaagcgttt acgagatgcg cgggccaaca gcgccggaac aattgccgcc
    26581 cagatggtag ccgctaagcc agcataacca atggcgtaca ggaatccgtt aggaaacagc
    26641 aggccaccca caacaggcgg ggcaaaggtc agcaacgctg ttttcaaacg gcccatagcc
    26701 gagtcgtcaa aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    26761 aacgaactcg ctaccgcaaa gtttgagaac acgaccagta gaagatccag actacggctg
    26821 ttcagtacgc cgcttaacgc ctgtaccagc acatcaatat taccgccctt ctcggcgatg
    26881 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    26941 gccagcgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    27001 taatacttca tcaggctcgg tacgttaccg tgataaccaa acgatgccag acagaacggc
    27061 agcgtcatca acagatacgg tgcataagac gcattgcttt cggcgacgtt gaacaatgtc
    27121 gcaggctgca catgccccag caggctaccg aaggtgagga agaaggtaat gactttcgcc
    27181 cccagcacaa tcgctgtcat gcgactgacg gctttggtgc tcaaccacac cacaaacgcc
    27241 accaacagtg caaaaccaaa acccgctgcc cgtgccggga cgtttagcga catctctgcg
    27301 aaggtgtgat gcagaatcga accactggca gaaatatagg cataggtcag gatgtagagc
    27361 acaaaggcaa tagaaatgcc gttgaccacg ttccagcctt tgccgagcag gtctttggtg
    27421 atggtgtcaa agctcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    27481 gaatgcagca tacagaacca ggtgaaaatc agcgccgcca ttgaccagaa gaaccacgcc
    27541 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    27601 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    27661 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacatga
    27721 gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat attattttac
    27781 agactgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    27841 gagtcatcac gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg acgcccatca
    27901 ccacgacccc cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    27961 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    28021 aactgcatgt tcatcggctt gttgagaatg cgagtacgcg taaagtgttg cagcacttca
    28081 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttaccg
    28141 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg acgaacttca
    28201 acaccatcat cgcggcccac ttcaatacgg tacaactgca tatcgccaac atcacgacgt
    28261 tcgcgacgcg gacgatcttc acggtcgcca cgcgggccac ggtcgttacg atcgcgcgga
    28321 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca
    28381 atcagagtac gttcaccctg tgccattttc agcagtgcgg cggccagagt ttcgagatcc
    28441 agctcttcac cttctgccgt cggctgaatt ttgctcagca gtgcacggta ttgatccaga
    28501 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg acgtttgccc
    28561 agcagttctg cgttcggcag ttcaacttcc ggaatggtca gcttcatggt gcgttcaatg
    28621 ttacgcagca gacgacgctc acggttctca acgaacagca gcgcacggcc agcacgaccc
    28681 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    28741 ttaaccacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc ggtcgcaatc
    28801 aggatgtcca gacgaccatc tttcagtcgt tccagtgtct gctcacgcag cgcctggttc
    28861 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc tgccacttcc
    28921 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    28981 aaacgtacca gtgcttcgtt tttacgcata ccccagacag tccagtagct ctggctgatg
    29041 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    29101 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    29161 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    29221 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    29281 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    29341 aattgcacgt cataacgttg gccgccgtac agggcaacca cgtttacgcc gcgcatgtgt
    29401 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    29461 accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg tagagagaat
    29521 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    29581 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    29641 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    29701 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    29761 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    29821 tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    29881 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    29941 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    30001 tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    30061 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    30121 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    30181 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    30241 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    30301 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    30361 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    30421 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat
    30481 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    30541 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    30601 gactggcaag gatctgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    30661 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    30721 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    30781 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    30841 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    30901 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    30961 ggcgatcaac ttccagcact ttcaccggta cttcctgacc catctgcagg taatcggtca
    31021 ctttctcaac gcgtttatca gcgatttgag agatgtggac cagaccttct ttaccgccac
    31081 caatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccattg tagacgcggc
    31141 ccacttcgat ttctgcagtg atctcttcga tacgacgaat cgcgtgtttc gctttctcgc
    31201 cgccggtcgc tgcgatcttc acagtaccgt catcttcaat ttcgatggtg gtgccggttt
    31261 cttcggtcag agcacggatt acagaaccgc ctttaccgat aacatctttg atcttgtccg
    31321 ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt
    31381 tgatcgcctg ttccattacc cccaggatat gcagacgcgc acctttagcc tggttcagtg
    31441 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagata
    31501 taccgtcacg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    31561 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    31621 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    31681 aagccataga agaggaaccg ttggattcgg taatttcaga cacaacacgt acggtgtacg
    31741 ggaatttgtc catatccggc attactgcca gcacgccgcg cttcgccaga cgaccgtgac
    31801 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    31861 agttgtagtg aaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    31921 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    31981 gcgcagaacc gtgagtacgc ggcagcacac cagtacgcac atccagacca cggatcatat
    32041 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacatttt
    32101 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    32161 gcagtgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    32221 cggtgatgcg gtaagcatcg ctcagacgag cttcagccag cgcagcaacg cgcgcgttca
    32281 gcgcttcgtt taccggctcc ggctgccagt cccaacgcgg tttaccggct tctttcacca
    32341 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    32401 gcatctgatc ttcgctcagc agttcagctt cagattcaac catcagcacg gcagcttctg
    32461 taccggcaac aaccaagtcc agtttgctct ctttcagttc atcctgagtc gggttcagta
    32521 cgtactggtc attgatgtaa cctacacggg cagcaccaat cgggccattg aacggaatac
    32581 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg
    32641 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    32701 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc
    32761 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    32821 taacggtcag cgggaagaag tcctgacctg gtttggcttt tttctgacca acaacggtaa
    32881 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    32941 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    33001 gcaatgtaat atcctttctc tttcttggac agtaccttac ggtactggtg ttaatacccg
    33061 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    33121 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    33181 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    33241 ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt
    33301 ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac
    33361 gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg
    33421 cagtcagcag tgctacctga acttcggtag aaccggtgtc gtttgcgtca cgaccaaact
    33481 cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat
    33541 agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta
    33601 aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacctgtt atcgcaagac
    33661 ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg
    33721 tcaatttcgc ccataccgat aaacttgccg ttctcaccct ctgtaacgcg aaccagtcct
    33781 tccagcggcg caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt
    33841 aacggaagat tcaccaccgg gtagtccgaa gctggactgt ccattggcat cagtaatgga
    33901 tcaagtaact ccgcagccgg aatatcctgc tgttcggctt gctcaaccag ttcacgcaga
    33961 tgttccaggg tcaccatccg ttcaaccgga tatttactta ccgccagacg acgcaagtaa
    34021 ataacatgcg cgccacaacc gagtttttcg ccgagatcgt caatgatagt gcggatataa
    34081 gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcatggcg aataaacagc
    34141 aattcataaa cggtaatcgg gcgcgcttca cgtggaactt caatgccctg acgcgcgtat
    34201 tcgtacagtt ttttgccctg atatttcagt gctgaataca tcgaagggat ctgttcgata
    34261 tcgccacgga aggtatccag cgccgccgcc agttgctctg cactaaaggt taccggacgt
    34321 tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac gctgcccaag acgcgcaatg
    34381 acccgatagc gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg
    34441 caaattggca acatgccggt cgccagcggg tccagcgcac cggtatgccc ggcacggttg
    34501 gcgttataaa tccgtttcac tttttgcagc gcatcgttgc tggacatccc ctgcggttta
    34561 tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct
    34621 ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca
    34681 ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca
    34741 cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcctgca
    34801 acgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac gtcacatata
    34861 ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca
    34921 gacgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg
    34981 ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg
    35041 ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa
    35101 gacgcgccag cgtcgcatca ggcaatccat gcaggatgcg acataaacgc cttatcccgc
    35161 atggaaccct aaaaacctta agcgatagta cgttggatct cgatgatttc gaatacttcg
    35221 atcacatcgc cagtgcggac atcgttgtag ttcttaacgc cgataccaca ttccataccg
    35281 ttacggactt cgttaacgtc atctttgaag cgtcgcaggg actccagctc gccttcgtag
    35341 ataaccacgt tgtcgcgcag aacgcggatc gggttgtgac gtttaaccac accttcggta
    35401 accatacaac ctgcgatagc accaaatttc ggtgatttga acacgtcacg aacttccgcc
    35461 agaccgataa tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact
    35521 tcgtcgatca ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc
    35581 actttacgtg cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc
    35641 gcagccaggg tagcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact
    35701 ttaacttcgt cagtagacag tttcagcaag gagtcggaaa ttgcttccac agaaccctgt
    35761 acgtctgcct tcaggacgat attcacttcg tgaacttcgc cttcggtcat gttggcgaac
    35821 atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga
    35881 cgatagagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc
    35941 gcagccggta cgccggacag gccaaggatt tccaccggaa tggacggacc cgcttccagc
    36001 acttcctgcc ccagttcgtt acgcattgca cgaacacgac cgtattcgaa gccacacaga
    36061 acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga
    36121 cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc
    36181 agctccagaa cttccgcctg cagcaggata gcatccagca gttcatcaat accggtaccc
    36241 gcttttgcag atacgtgtac gaactggctt tcgccgcccc actcttctgg caggatgccg
    36301 tactgggaga gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gattttgttc
    36361 acagcaacca ccaccggaac acccgccgct ttcgcgtgct ggatagcttc gatggtctgc
    36421 ggcattacac cgtcgtctgc agcaacaacc aggactacga tgtccgttgc ctgcgcacca
    36481 cgtgcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg
    36541 ttttcagttt caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag
    36601 gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc
    36661 atgatggtca caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt
    36721 accgcctctt ccagctcgtt ttcacgacgc aggataactt tgtggcccat ctcttcagca
    36781 accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc
    36841 atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg
    36901 gtgatggttt cgccgatcac aacgtcacgg ttaacggcct gagctggctt ctggaagcct
    36961 tgctgcagcg aagaaccttt acgttttccg cccttaccgc cacgtactgc tgcgcgagct
    37021 tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc
    37081 gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg
    37141 cgagcatgtt gagaagtagt gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta
    37201 tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct
    37261 tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc ggctttacgc
    37321 ttgagctctg cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata
    37381 tcgtcttgtt gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca
    37441 gcttcacgct tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta
    37501 gccgattctt ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc
    37561 gctgcaaggc gctcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact
    37621 tcgatttgta ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt
    37681 ttacgttgca gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa
    37741 gtctgtttct cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca
    37801 aattgctgta ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt
    37861 acatctgtca tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa
    37921 atattacggg cagccataat cagtgctccg gctttttcgt cggtcaaccc ttcgatatca
    37981 gccagatcat caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc
    38041 agtttgaatg ccaaatcacg atctacccct tcaaggttca gcagatcgtc agccggtttg
    38101 ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc
    38161 tcgcgcagtg cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc
    38221 ggcacatagg ccagttcttc cagcgtcgag aagccttctt ctaccagaac agtcgcgaag
    38281 tcttcatcga tatcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga
    38341 tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt cccaaccgct cagctgcgaa
    38401 gccagacgca cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg
    38461 gcgatatcca tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc
    38521 attgcgttaa tcacaaactg cgccgggtta tcatcccaca ggacaatatc gatacgctcg
    38581 ccgcccagtt cagtagaaac cgcctgaaca cgcgcgccac gcatacctac gcaagcacct
    38641 accggatcga tacgtttatc gttggttttc accgcaattt tcgcacgaga tcccgggtcg
    38701 cgagccgctg ctttaatttc aatcacttct tcgccgattt ccggcacttc aatacggaac
    38761 agttcgatca gcatttccgg cttggagcgg gtgacgaaca gctgcgcgcc acgcgcttcc
    38821 gggcgaacgg aatagagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc
    38881 agcatatctt cgcgcaggat cacggcttca gcgttgttgc ccagatccag agagatgttg
    38941 tcgcggttta cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga
    39001 tcaaccacca tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc
    39061 tgggtagtga tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccagg
    39121 ttcaggcttt catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg
    39181 acttcatcaa caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc
    39241 tgtacgcgaa cgtcgatttc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt
    39301 tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact
    39361 acagccaaaa tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg
    39421 ggaaccaggt tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg
    39481 gtaactgtga tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt
    39541 tgtaccgcca tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca
    39601 gccgtgaaca gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg
    39661 atggggtctt caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca
    39721 acattgatgc catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata
    39781 aattcgatgc caaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt
    39841 aatttttgct ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc
    39901 agttattctg tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac
    39961 cctataaccg caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac
    40021 gaaggccgaa gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg
    40081 gccggatttg aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac
    40141 cccgcgcctg aaacgtggca aattctactc gttttgggta aaaaatgcaa atactgctgg
    40201 gatttggtgt accgagacgg gacgtaaaat ctgcaggcat tatagtgatc cacgccacat
    40261 tttgtcaacg tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa
    40321 tacttttacc ttctcctgct ttcccttaag cgcattattt tacaaaaaac acactaaact
    40381 cttcctgtct ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca
    40441 gttagagcag aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga
    40501 cgattctcaa gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg
    40561 acaccagtgc cgcactgctg tggatgcgac aaaagggagc tgttccttat gcatatactg
    40621 caaacctggg ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat
    40681 acggcgcgga gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg
    40741 ccgctattca gtgtggcgca tttcataaca ccactggtgg actaacctat ttcaacacga
    40801 cgccgctggg ccgcgccgtg accggcacca tgctggttgc tgctatgaaa gaagatggcg
    40861 tgaatatctg gggtgacggc agcacctata aaggaaacga tatcgaacgt ttctaccgtt
    40921 acggtctgct gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta
    40981 ttgatgaact gggtggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact
    41041 acaaaatgtc tgtcgaaaaa gcttactcca cggactccaa catgcttggt gcaacgcatg
    41101 aagcgaagga tctggaatac ctcaactcca gcgtcaaaat cgtcaaccca attatgggcg
    41161 tgaagttttg ggatgagagc gtgaaaatcc cggcagaaga agtcacagta cgctttgagc
    41221 aaggtcatcc ggtggcgctg aacggtaaaa cctttagcga cgacgtagaa atgatgctgg
    41281 aagctaaccg catcggcggt cgtcacggcc tgggcatgag cgaccagatt gaaaaccgta
    41341 tcatcgaagc aaaaagccgc ggcatttatg aagcccctgg gatggcactg ctgcacattg
    41401 cgtatgaacg tttgttgacc ggtattcaca acgaagacac cattgagcag tatcacgcgc
    41461 atggtcgtca gttgggccgc ctgctgtacc agggacgttg gtttgattcc caggcgctga
    41521 tgctgcgtga ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttactctgg
    41581 agctgcgccg cgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca
    41641 agccagagcg tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtatcg
    41701 gtcaactgac catgcgtaac ctggatatca ccgatacccg cgagaaactt ttcggttatg
    41761 ccaaaactgg cctgctctcc tcctctgccg cttcaggggt gccgcaggtg gagaatctgg
    41821 aaaataaagg ccagtaattc gacatcaacc ctgcccttca ggggtggggt taccttgatt
    41881 atcctctgta aactcctttc tgagcatcac ataatcgaaa acatcatggt ttgcatccac
    41941 aacccgtgag tctcctttat agtgtgcaat cacttcctca agcgtttgcg gctgttccgg
    42001 ctttgttacc cccatccacg cattaataag gtaattattg taagctgtcg aaaagatgtt
    42061 gttttccgtt gtgcgatcca cgccatcccc tagaacgggg ctataccaga taaacatcgg
    42121 gacatgatac gcctgctggc tagcctccct gcctccatga aaatagacgt tcttcttcgt
    42181 agggtcgcgt tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa
    42241 taattcaaaa acctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc
    42301 atcctgatcg tcctgcggtt gaaacacagc gctggattgc ggataggcgc tacaagccgg
    42361 ttcatggctt ccatttaaat gaagaacaat cagtttcttt tgctgcgtct tttgctgtaa
    42421 cgcttgcgat aaatgcggca acaacaattc atcaaatcct ctgacataga ctgtttccat
    42481 ggcgcgcatg gcgatactgg taactgctgt accattctgc cgaaaagcgg attgcgagct
    42541 tagccagaaa gtctgaaatc ctgcttgatt agccatatta ataatgttgt ccgggtaatt
    42601 atgaatgtca tgactcaaaa cagaatcagc agttaaagaa aggggaaccg acagcgcagt
    42661 gtaaggtgca ccgcttattg cttgattaaa cagtttgatc tgctttcttt gtgcttcgac
    42721 ttgcggtgtc gtagagcgtg tatatccata caaagacata ttgtcgacac gtacagactc
    42781 ccccacaatc aacacgtagg tatcaatacc tgtatccctg actgatagtt gaaaatacgg
    42841 caccgtgttt gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaataatt
    42901 gagattgaaa aacggcgtat aggtagcaaa tcgcgacgct agtatatatg gactgaacgc
    42961 ctctttattt tttgcatctt tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat
    43021 gacaatcagc aataatattc ctgtcacttt ttttgtcggc aaggaaacat catattttat
    43081 tattactgca aaaaaaagaa gggataaaaa ggcaaaagca catagataag ggatatacat
    43141 tcccagcatt ttgacaactt catccggatc actctgcagc acactaatcg caaatccatc
    43201 attgaaagtt gtaccaaaga tacaccatga atagaggcta atactcatat ctgccgcaat
    43261 cagtaacaca aatggtacgg caataagcag gcgaagcgga agggatgata tacgtttaac
    43321 aaccaggatg gtcagaacga agaacagcaa tcgctgaacc atatgcgctc cggaagaagc
    43381 gactaagttc gtaataccaa aaacaataac acaaagatac aacaaccaat gagatttaaa
    43441 acttttaaca aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa
    43501 caattatcaa caatgttcaa ttttata
//
LOCUS       DAZFRG010000007        42774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFRG010000007 DAZFRG010000000
VERSION     DAZFRG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.007"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..390)
                     /gene="yeiL"
                     /locus_tag="ACHKUC_000264"
     CDS             complement(<1..390)
                     /gene="yeiL"
                     /locus_tag="ACHKUC_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO6616596.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCV"
     gene            520..1461
                     /gene="rihB"
                     /locus_tag="ACHKUC_000265"
     CDS             520..1461
                     /gene="rihB"
                     /locus_tag="ACHKUC_000265"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO6616597.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1561..1626
                     /locus_tag="ACHKUC_000266"
                     /pseudo
     CDS             1561..1626
                     /locus_tag="ACHKUC_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1729..2817)
                     /locus_tag="ACHKUC_000267"
     CDS             complement(1729..2817)
                     /locus_tag="ACHKUC_000267"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6616598.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPTHTTVDSFGADDGFMAGLVYSFLEGYSFRDSAHFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(2821..3678)
                     /gene="nfo"
                     /locus_tag="ACHKUC_000268"
     CDS             complement(2821..3678)
                     /gene="nfo"
                     /locus_tag="ACHKUC_000268"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO6616599.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFAGIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(3752..4801)
                     /gene="yeiH"
                     /locus_tag="ACHKUC_000269"
     CDS             complement(3752..4801)
                     /gene="yeiH"
                     /locus_tag="ACHKUC_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO6616600.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            4900..5781
                     /gene="yieE"
                     /locus_tag="ACHKUC_000270"
     CDS             4900..5781
                     /gene="yieE"
                     /locus_tag="ACHKUC_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO6616601.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            5986..7455
                     /gene="lysP"
                     /locus_tag="ACHKUC_000271"
     CDS             5986..7455
                     /gene="lysP"
                     /locus_tag="ACHKUC_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO6616602.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            7747..9738
                     /gene="cirA"
                     /locus_tag="ACHKUC_000272"
     CDS             7747..9738
                     /gene="cirA"
                     /locus_tag="ACHKUC_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO6616603.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(9770..10606)
                     /gene="yeiG"
                     /locus_tag="ACHKUC_000273"
     CDS             complement(9770..10606)
                     /gene="yeiG"
                     /locus_tag="ACHKUC_000273"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6616604.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            10864..11532
                     /gene="folE"
                     /locus_tag="ACHKUC_000274"
     CDS             10864..11532
                     /gene="folE"
                     /locus_tag="ACHKUC_000274"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO6616605.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11549..12706
                     /gene="yeiB"
                     /locus_tag="ACHKUC_000275"
     CDS             11549..12706
                     /gene="yeiB"
                     /locus_tag="ACHKUC_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO6616606.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKHWKLHSGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSFRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            12848..13888
                     /gene="galS"
                     /locus_tag="ACHKUC_000276"
     CDS             12848..13888
                     /gene="galS"
                     /locus_tag="ACHKUC_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO6616607.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14168..15166
                     /gene="mglB"
                     /locus_tag="ACHKUC_000277"
     CDS             14168..15166
                     /gene="mglB"
                     /locus_tag="ACHKUC_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO6616608.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15227..16747
                     /gene="mglA"
                     /locus_tag="ACHKUC_000278"
     CDS             15227..16747
                     /gene="mglA"
                     /locus_tag="ACHKUC_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO6616609.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            16763..17773
                     /gene="mglC"
                     /locus_tag="ACHKUC_000279"
     CDS             16763..17773
                     /gene="mglC"
                     /locus_tag="ACHKUC_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO6616610.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18016..19251)
                     /gene="preA"
                     /locus_tag="ACHKUC_000280"
     CDS             complement(18016..19251)
                     /gene="preA"
                     /locus_tag="ACHKUC_000280"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO6616611.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(19245..20483)
                     /gene="preT"
                     /locus_tag="ACHKUC_000281"
     CDS             complement(19245..20483)
                     /gene="preT"
                     /locus_tag="ACHKUC_000281"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO6616612.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            complement(20677..20916)
                     /gene="yeiS"
                     /locus_tag="ACHKUC_000282"
     CDS             complement(20677..20916)
                     /gene="yeiS"
                     /locus_tag="ACHKUC_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO6616613.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(20919..21638)
                     /gene="sanA"
                     /locus_tag="ACHKUC_000283"
     CDS             complement(20919..21638)
                     /gene="sanA"
                     /locus_tag="ACHKUC_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO6616614.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(21788..22672)
                     /gene="cdd"
                     /locus_tag="ACHKUC_000284"
     CDS             complement(21788..22672)
                     /gene="cdd"
                     /locus_tag="ACHKUC_000284"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO6616615.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(22802..23497)
                     /gene="yohK"
                     /locus_tag="ACHKUC_000285"
     CDS             complement(22802..23497)
                     /gene="yohK"
                     /locus_tag="ACHKUC_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO6616616.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHALLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(23494..23892)
                     /gene="yohJ"
                     /locus_tag="ACHKUC_000286"
     CDS             complement(23494..23892)
                     /gene="yohJ"
                     /locus_tag="ACHKUC_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6616617.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            24131..25081
                     /gene="dusC"
                     /locus_tag="ACHKUC_000287"
     CDS             24131..25081
                     /gene="dusC"
                     /locus_tag="ACHKUC_000287"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO6616618.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(25469..25552)
                     /gene="yohP"
                     /locus_tag="ACHKUC_000288"
     CDS             complement(25469..25552)
                     /gene="yohP"
                     /locus_tag="ACHKUC_000288"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO6616619.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            25776..27212
                     /gene="mdtQ"
                     /locus_tag="ACHKUC_000289"
                     /pseudo
     CDS             25776..27212
                     /gene="mdtQ"
                     /locus_tag="ACHKUC_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            27265..28026
                     /gene="yohF"
                     /locus_tag="ACHKUC_000290"
     CDS             27265..28026
                     /gene="yohF"
                     /locus_tag="ACHKUC_000290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6616620.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     RKVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(28146..28724)
                     /gene="yohD"
                     /locus_tag="ACHKUC_000291"
     CDS             complement(28146..28724)
                     /gene="yohD"
                     /locus_tag="ACHKUC_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6616621.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            28894..29481
                     /gene="yohC"
                     /locus_tag="ACHKUC_000292"
     CDS             28894..29481
                     /gene="yohC"
                     /locus_tag="ACHKUC_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO6616622.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            29655..30587
                     /gene="pbpG"
                     /locus_tag="ACHKUC_000293"
     CDS             29655..30587
                     /gene="pbpG"
                     /locus_tag="ACHKUC_000293"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO6616623.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTVAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(30626..32341)
                     /gene="dld"
                     /locus_tag="ACHKUC_000294"
     CDS             complement(30626..32341)
                     /gene="dld"
                     /locus_tag="ACHKUC_000294"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6616624.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     VLKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVK"
     gene            32537..34834
                     /gene="bglX"
                     /locus_tag="ACHKUC_000295"
     CDS             32537..34834
                     /gene="bglX"
                     /locus_tag="ACHKUC_000295"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO6616625.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            35024..35941
                     /gene="osmF"
                     /locus_tag="ACHKUC_000296"
     CDS             35024..35941
                     /gene="osmF"
                     /locus_tag="ACHKUC_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO6616626.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQIAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            35948..37105
                     /gene="yehY"
                     /locus_tag="ACHKUC_000297"
     CDS             35948..37105
                     /gene="yehY"
                     /locus_tag="ACHKUC_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO6616627.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGTQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            37098..38024
                     /gene="yehX"
                     /locus_tag="ACHKUC_000298"
     CDS             37098..38024
                     /gene="yehX"
                     /locus_tag="ACHKUC_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO6616628.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            38029..38760
                     /gene="yehW"
                     /locus_tag="ACHKUC_000299"
     CDS             38029..38760
                     /gene="yehW"
                     /locus_tag="ACHKUC_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO6616629.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRVR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(38741..38848)
                     /gene="yohO"
                     /locus_tag="ACHKUC_000300"
     CDS             complement(38741..38848)
                     /gene="yohO"
                     /locus_tag="ACHKUC_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO6616630.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(38908..39639)
                     /gene="mlrA"
                     /locus_tag="ACHKUC_000301"
     CDS             complement(38908..39639)
                     /gene="mlrA"
                     /locus_tag="ACHKUC_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO6616631.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            39861..41546
                     /gene="btsS"
                     /locus_tag="ACHKUC_000302"
     CDS             39861..41546
                     /gene="btsS"
                     /locus_tag="ACHKUC_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO6616632.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGGDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            41543..42262
                     /gene="btsR"
                     /locus_tag="ACHKUC_000303"
                     /gene_synonym="yehT"
     CDS             41543..42262
                     /gene="btsR"
                     /locus_tag="ACHKUC_000303"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO6616633.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            42309..>42774
                     /gene="yehS"
                     /locus_tag="ACHKUC_000304"
     CDS             42309..>42774
                     /gene="yehS"
                     /locus_tag="ACHKUC_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO6616634.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKK"
BASE COUNT        10372 a        10585 c        11356 g        10461 t
ORIGIN      
        1 gacgcagagt tttcgtaaaa atagcgtgtc gtttaataac aacgggcggt aatgcttcat
       61 agggagcgca aggcaccaac actcttcaat agcctgcact gcacgtggtt catggtcttt
      121 gtcgattaac tcaatctcgc caataaaaca gggcgcggca aagaaatcga tcagcgacac
      181 gcgaccatta gcaagcgtga cataaagcct ggcgcggcct cgcgtcaggt aaaacagcca
      241 ggacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa acagccgcgt
      301 atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca tcaattgctg
      361 cttaagatca ttattgtgga tttctttcac gatgtttatc cgctatgaga tttctcatat
      421 ttagcatacg tacgctgttg ttaagattca atcatcggcc aggtcgaata ttctttgctg
      481 tagaaacaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag aaaaattatt
      541 ctggattgtg atccgggtca tgatgatgct attgctataa tgatggcggc gaaacatccg
      601 gcgattgatt tattaggtat caccattgta gcgggtaatc aaaccctcga taaaacatta
      661 attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta tgcggggatg
      721 ccgcagccca ttatgcgtca acaaattgtt gccgataata ttcacggtga aaccggactg
      781 gatggcccgg tattcgagcc gctgacccgc caggcagaaa acactcatgc ggtgaaatat
      841 atcatcgata cccttatggc aagcgatggc gatatcactc tggtgccggt tggtccgttg
      901 tcaaacatcg cggtggcaat gcgtatgcaa cccgcgatcc tgccgaaaat ccgtgaaatt
      961 gttctgatgg gcggtgctta tggtactggc aacttcaccc catctgctga gttcaacatc
     1021 tttgctgacc cggaggccgc acgtgtggtg ttcacctccg gcgttccatt agtgatgatg
     1081 ggcctcgatc ttaccaacca gaccgtttgc accccggatg tgattgctcg gatggaaagg
     1141 gctggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag
     1201 ttcgaaaact acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatttg
     1261 attaaccctg atggcattaa aacccaggaa atgtacgtcg aagtggacgt taacagtggc
     1321 ccttgctatg ggcgtaccgt ctgcgacgag ctgggtgttc ttggcaagcc cgccaatacc
     1381 aaagtcggca tcactattga taccgactgg ttctggggat tagtcgaaga gtgcgtacgc
     1441 ggctacataa aaacccatta agtgttgata acaagccggg tcagtatcgc aagatgcagg
     1501 cccggctatt tatgatgggc agagagccgt cgcccaaaat gacatgaaga gataataact
     1561 atggatgtca tgagaagtgt tctgggaatg gtggtattac tggctattgc gtttttgctg
     1621 tcagtaacct gatgagcgct actattgcag gattctttat tggtttagcg tagtgcgcaa
     1681 tttatgagcc ggataagatg cggtcaagcg tcgaatccgg caacattgtt acaccattgg
     1741 cactaatgaa agcgcgttat cggcagacag ggtagggttg tttaagctgc cgctggcgcg
     1801 cgaaattgcc gcgcaggcta tcgcaaaatg ggcgctgtcg cggaaactgt atccttcaag
     1861 aaagctatat accaggcccg ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt
     1921 atgcgttggc gcggtcagca aaaattgttc tccatcctta tcgctgcaaa atacggattc
     1981 atctttcaga taaacaaaaa tgcgtttaac accttgttga tgcagcgaat ttacagctct
     2041 gatgcgatcg ttgtcgtctt taatcggttg cccccacaaa atttccagtt cattttgtgt
     2101 cggtttcaga gtgtggatcc ggctgtacca gttttttact ttgttcgctt taaattcgga
     2161 aacggtatcg acaaacatcg gaatttcatc agcaatggta aagacccact ccagggcttc
     2221 aggtgtgaga ttacaatcag ccagcaccac accagcatgg cgaatcaagt cccgtgaagt
     2281 attgagcagt tgaggcgtta gctgttgcag aatatgtgtg tcattgattg ccaggatagt
     2341 ttcttcctgc ttattagcga tagcgagata agttgcagtg ctgtgtccat gcagacgaat
     2401 gcagtttgaa acgttcacgc ccgcgcggcg cgtttcttcc agtaatgtct cgccgtaaaa
     2461 atcgttgcca atagctgaaa ttaaatgcac atcccgacca agcagagcca ggttatgcgc
     2521 gatattacgt ccgacgccgc ctgcggaaca gtgaacagaa cctggatgag aggctgcttg
     2581 cgggtaacgg atatccgcca ttccgcgaat atccatattg attgcaccca caaccacaca
     2641 gtaatcctgc tcggtaagga tatatccttt gcctttaatc aaacccttgc gcatcaaatc
     2701 cataatatga gcggcaacac gtgagcggct gatttgcaga atatcggcga tttcattctg
     2761 ttgaatcagc gggttacgcc gtaaaatttt aagaatctgc ttttccctgt cgttcatcga
     2821 tcaggccacc gctttttcag tttgttgcgc tttcagccag gcgatctctt ctgcccagat
     2881 atccgggttg atggtttcaa ggatcagcgg aatgccgtcg aaacggtcgt cttgcatgat
     2941 ccagcggaac gcatcatgac cgatattgcc ttcgccgagg ctatgatgac ggtcaacgcg
     3001 gctgccaaag gtgcttttcg catcgttgag gtgcatcccg cgcagatact taaagccaac
     3061 aataccggca aaatcagcga acgttttctc gcattcggca ggagtacgca aatcataccc
     3121 ggcagcgaaa gcatggcagg tatcaatgca gacgccgacg cgggatttat cttccacgcc
     3181 gtcgataatc gctgcgagat gttcgaattt aaaccctaag ttactgccct gaccggcggt
     3241 gttttcaatc accgcggtga caccttgagt tttatccagc gcaatgttga tggattcggc
     3301 aatacgcgca aggcaatcct cttctgaaat ctgcatcaga tggctgccag ggtggaagtt
     3361 gagcaaagaa agtcccagtt gttcgcaacg ctgcatttca tctataaagg catcgcgcga
     3421 tttttccaga gcttcagcaa ccggatgtcc gaggttaatc agataactgt cgtggggaag
     3481 aatttgcgcc gatgtgtagt gatatttttc acaggcggct ttgaattcat cgatggtttg
     3541 cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg gtgaacaagg caaacgcggt
     3601 tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga ccgccagcag cactaacgtg
     3661 cgctccaatg tatttcatgc gaggactcct gttaaacccg ctggaggaaa acggtaatga
     3721 tagcgggtta acaggagcaa gatgtagtgg tttatgcgat gacgctttga atcacatagt
     3781 taatcgcacc accaccaaca atcagccagg caaacagtac cagtgccatc agcagaggtt
     3841 tcgccccggc ttttttcagc gcgctgacgt gagtggtcag acccagcgcc gccatcgcca
     3901 ttgccagcag gaaggtatcc agcgttacca gcatgttcac cacgctctgc ggtaacaggt
     3961 ggaacgagtt aaagatggca acgacgatga acaagatggc aaaccacgga atagtgattt
     4021 tgcttttctc gccgctgttc gccccagaca gctgtttaac acgcgccgcc agcaggatga
     4081 ggaacggagc cagcatcatc acgcgcagca ttttggaaat aactgctgcg ttttctgcat
     4141 ccgggctgat ggcatgacct gccgccacca cctgcgccac ttcgtgcaca gtagaaccaa
     4201 tgtagatacc gaaagtttcc ggactaaacc attgagacat cagcggatat atcgccgggt
     4261 agaggaaaat cgcgacggtc ccgaagataa caacggttgc aacagccacg gttactttac
     4321 tggcttctgc ttttactacc ggctcagtcg ccagtaccgc ggcagcacca cagatactgc
     4381 taccggcacc gatcaaccag ctggtgtgct tatccagacc aaacactttc tgccccagga
     4441 agcaagccag caggaaggta ctggacagcg tcaacacgtc aatgatgatc ccactgatac
     4501 cgacatcggc aatttgcgag aacgtcagac ggaagccata aagaatgata cccagacgta
     4561 ataaatattg cttggcaaac agcacaccac cgtcacagct tttccagatg tgcgggtaaa
     4621 tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt gagggcacta aacccggcac
     4681 ccgcaacagc gggaatggaa ccaccccaca gggcgacccc ggtgataact gcactcaggg
     4741 ctaaccccgg aataaaatgc cacagtgtac gatgttgttt ctgtaaggtg atattcgtca
     4801 taaccctctc ctttaaccgg gcataaggtt acgactaact ggtttaaaaa taaaattgat
     4861 tatatattta taattaatct ttataagtgg taagcgacta tgcacatcac cctccggcag
     4921 ttggaagttt ttgcagaagt attgaaaagt ggatcaacca cccaggcgtc ggtgatgctg
     4981 gcgttgtcgc aatcagcagt gagcgcagcc ttgaccgacc tggaagggca gcttggcgtt
     5041 caactgtttg atcgcgtggg gaaaagactg gttgttaatg aacacgggcg gctgctctat
     5101 ccgcgtgcgt tggcactgct tgaacaggcg gttgaaatcg aacaactgtt tcgcgaagac
     5161 aacggcgcga ttcgtatcta tgccagtagt accatcggta actacattct gcctgcagtt
     5221 atcgcccgtt atcgccatga ttatccgcag ttgccgattg aacttagcgt tgggaatagc
     5281 caggacgtga tgcaagcggt gctggatttc cgcgttgata ttggctttat tgaaggaccg
     5341 tgccacagca ctgaaatcat ttctgaaccg tggctggaag acgagctggt ggttttcgcc
     5401 gcgccgactt cgccgttggc tcgtggtccg gtcaccttag aacagctggc cgctgcgccg
     5461 tggatcctgc gtgaacgcgg ttccggcacg cgggagattg tcgattatct gttgctgtca
     5521 catttaccga agtttgagat ggcgatggaa ttaggtaact ccgaggcaat caaacatgcg
     5581 gtgcgtcatg ggttgggaat tagttgcctg tcgcgacggg tgattgaaga tcaattgcag
     5641 gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc tgatgcgtac gttgtggcgt
     5701 atacatcatc ggcaaaaaca cctttccaac gcgctacggc gctttctgga ctattgcgat
     5761 cccgcgaatg taccgcgtta aggtgctgta caagaacatg ctggtgctgt gtcgatttcg
     5821 tgacgcagcg ccctcagcat gcattcgcca gaaaagagat tggctgcttt acttataatc
     5881 cctgggcgat catgaaggtg tcttataacc gtgtatttct gccggaagga ttgccaatcg
     5941 tctgctacaa tcgcgcctca tttttaagat ggatagcatt tttgtatggg ttccgaaact
     6001 aaaactacag aagcgccggg cttacgccgt gaattgaagg cgcgtcacct gacgatgatt
     6061 gccattggcg gttccatcgg tacaggtctt tttgttgcct ctggcgcaac gatttctcag
     6121 gcaggtccgg gcggggcatt gctctcgtat atgctgattg gcctgatggt ttacttcctg
     6181 atgaccagtc tcggtgaact ggctgcatat atgccggttt ccggttcgtt tgccacttac
     6241 ggtcagaact atgttgaaga aggctttggc ttcgcgctgg gctggaacta ctggtacaac
     6301 tgggcggtaa ctattgccgt tgacctggtt gcagcacagc tggtcatgag ctggtggttc
     6361 ccggatacac cgggctggat ctggagtgcg ttgttcctcg gcgttatctt cctgttgaac
     6421 tacatctctg ttcgcggctt tggtgaagcg gaatactggt tctcactgat caaagtcacg
     6481 acagttattg tctttatcat cgttggcgtg ctgatgatta tcggtatctt caaaggcgcg
     6541 cagcctgcag gctggagcaa ctggacaatc ggcgaagcgc cgtttgctgg tggttttgcg
     6601 gcgatgatcg gcgtagctat gattgtcggc ttctctttcc agggaaccga gctgatcggt
     6661 attgctgcag gcgagtccga agatccggcg aaaaacattc cacgcgcggt acgtcaggta
     6721 ttctggcgaa tcctgttgtt ctatgtgttc gcgatcctga ttatcagcct gattattccg
     6781 tataccgatc cgagcctgct gcgtaacgat gttaaagaca tcagcgtcag tccgttcacc
     6841 ctggtgttcc agcacgcggg tctgctctct gcggcggcgg tgatgaacgc agttattctg
     6901 acggcggtgc tgtcagcagg taactccggt atgtatgcgt ctactcgtat gctgtatacc
     6961 ctggcgtgtg acggtaaagc gccgcgcatt ttcgctaaac tgtcgcgtgg tggcgtgccg
     7021 cgtaatgcgc tgtatgcgac gacggtgatt gccggtttgt gcttcctgac ctccatgttt
     7081 ggcaaccaga cggtatacct gtggctgctg aacacctccg ggatgacggg ttttatcgcc
     7141 tggctgggga ttgccattag ccactatcgc ttccgtcgcg gttacgtatt gcagggacac
     7201 gacattaacg atctgccgta ccgttcaggt ttcttcccac tggggccgat cttcgcattc
     7261 attctgtgtc tgattatcac tttgggccag aactacgaag cgttcctgaa agataccatt
     7321 gactggggcg gcgtagcggc aacgtatatt ggtatcccgc tgttcctgat tatttggttc
     7381 ggctacaagc tgattaaagg aactcacttc gtacgctaca gcgaaatgaa gttcccgcag
     7441 aacgataaga aataagtttc ctcgcttcct tgctaagccc tctcaaccga gagggctttt
     7501 tcaattccat ttccctgaca aatcatgcgg atataaaatt taacatttgg attgataatt
     7561 gttatcgttt gcattatcgt tacgccgcaa tcaaaaaagg ctgacaaatc agaggctgtt
     7621 ccggctttct gggatgatca cctgcataca aataagtcca ccgcgatgct gccgtacgca
     7681 aggggacgta aagaagatgt gagcgatacc cattttattt tcgtagttac ctcatggaga
     7741 tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc tgtgtttgtc cgctatttct
     7801 tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct
     7861 tccgttgaac aaaaccttaa agatgctccc gccagtatca gcgtcattac ccaggaagac
     7921 ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa
     7981 ctgacgaacg aaggggataa ccgtaagggc gtaagtattc gtggtctgga cagcagctac
     8041 accctgattc ttgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat
     8101 gatttcgatc tgaactggat cccggtcgat tccatcgagc gtattgaagt ggtccgtggc
     8161 ccgatgtcgt cgctgtacgg ttccgatgcg ctcggcggtg tagtgaatat catcaccaaa
     8221 aaaatcggtc agaaatggtc gggcaccgtt accgtcgata ctaccgttca ggaacatcgc
     8281 gatcgcggtg atacctataa cggtcaattc tttaccagcg gaccattaat tgacggcgtg
     8341 ctgggaatga aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca
     8401 acgaccaccg ataccggaga aacgccgcgt attgaaggat tctccagccg cgacggcaat
     8461 gtcgaatttg cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac
     8521 cgtcaggatc gtgattccga ctcgctggac aaaaaccgcc tggaacgcca gaactactcc
     8581 gtcagccata atgggcgttg ggattacggc accagcgaac tgaaatacta cggtgagaaa
     8641 gtcgagaaca aaaaccctgg caacagcagc ccgataactt ccgaaagcaa tacggtcgac
     8701 ggcaaataca cgttgccgct gacggcgatt aatcagtttc tcacggttgg cggtgaatgg
     8761 cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg gaaccagctc caaaacgtct
     8821 gccagccagt acgcgctgtt tgtggaagat gaatggcgga tcttcgagcc gctggcgctg
     8881 acgaccggcg tgcgtatgga cgatcacgaa acctacggtg aacactggag tccgcgtgcc
     8941 tacctggttt ataacgccac cgacaccgta acggtgaaag ggggctgggc gacggcattt
     9001 aaagcccctt ctctgttgca acttagccct gactggacga gcaattcctg ccgtggcgca
     9061 tgtaagattg tgggtagccc ggatctgaaa ccagaaacca gcgaaagttg ggagctgggg
     9121 ctttactaca tgggtgaaga aggctggctg gaaggggttg aatccagcgt taccgttttc
     9181 cgtaacgatg tgaaagatcg tatcagcatt agccgtacgt ctgacgtcaa tgctgcaccg
     9241 ggctaccaaa actttgtcgg ttttgagacg ggcgctaacg gacggcgcat accggtattt
     9301 agctactaca acgttaacaa agctcgtatt cagggcgtgg aaaccgaact gaaaattccg
     9361 ttcaacgatg aatggaaact gtcgatcaac tacacctaca acgatggtcg tgatgtcagc
     9421 aacggcgaaa acaaaccgct atccgatctg ccgttccata ctgctaacgg tacgctggac
     9481 tggaaaccgc tggcgctgga agactggtca ttctatgttt ctggtcacta taccgggcag
     9541 aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt ataccatctg gaataccggc
     9601 gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag gcgtgctgaa ccttggcgac
     9661 aaggatctca gtcgtgacga ctacagctat aacgaagacg gacgtcgtta ctttatggca
     9721 gtggattatc gcttctgatg agaagatgcc cggcgaaccg ggcggacttt cacttcagta
     9781 aatactgcgc atggaagcgc aggtgatcct ctataaaaga ggcgatgaag tagtaactgt
     9841 gatcatatcc cggctgaata cgcagcgtca tcggccacgc tttctggcgc gcggcttctg
     9901 ccagtactgc aggttgcagt tggtcagcaa gaaactgatc attatcaccc tgatcaataa
     9961 gcgtcgggat tgcatcctgc gcgttactgg catacatcaa cgcgcaactg tcccattcca
    10021 gccatgcatt tttgtcctca cctaaataag tgctaaacgc tttgattccc cacgggacgc
    10081 tgcacggatt cacaattggc gcaaaggcgg aaacgctggt gtatttaccc ggatttttca
    10141 gcgccataat cagcgcaccg tgaccaccca tggaatgacc gctaatagcg cagcggtcgc
    10201 tgacattaaa ttgcgactga accagcgccg gtaattcatc gcgcagataa tcatacatcc
    10261 ggtaatgcgt cgcccacggt ggttgcgtgg cgttaagata aaaccccgcg ccctggccta
    10321 aatcgtagcc atcgtcgttg gcaacctgtt cgccgcgcgg gctggtgtct ggcatcacca
    10381 gtacaatccc cagttccgcc gccacgcgct gggcacccgc cttggtggtg aagttctcgt
    10441 cattgcaggt taatccggaa agccagtaca gcactggtgg cggggtgtga tcacgaggtg
    10501 gtgggagaaa gatactgaac gtcatcgggc agtttaaggt actggagtcg tgtcgccagc
    10561 gttgctgcca gccttcaaaa cagcggtgct cttcgagcat ttccatgcgt ggctccttgt
    10621 tgtgttgttt gcaatttctt catcataccg tttattcaat gtgctgtgag taactttcac
    10681 ttccgtattt gcataacgat gttttaacat ctgctgatga aaggcagcgg caattacaat
    10741 aattatcgct gtgaatactg gattatgtgc gccgcctcac gcacaataat caggctgtaa
    10801 atcagcttaa taactttgcc cccacgcagg gcggaggcgt cacacctgca ggagaaatca
    10861 taaatgccat cactcagtaa agaagcggcc ctggttcatg aagcgttagt tgcgcgagga
    10921 ctggaaacac cgctgcgccc gcccgtgcat gaaatggata acgaaacgcg caaaagcctt
    10981 attgctggtc atatgaccga aatcatgcag ctgctgaatc tcgacctggc tgatgacagt
    11041 ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg atgaaatttt ctccggtctg
    11101 gattacgcca atttcccgaa aatcaccctc attgaaaaca aaatgaaggt cgatgaaatg
    11161 gtcaccgtgc gcgatatcac tctgaccagc acctgtgaac accattttgt taccatcgat
    11221 ggcaaagcga cggtggccta tatcccgaaa gattcggtca tcggtctgtc aaaaattaac
    11281 cgtattgtgc agttctttgc ccagcgtccg caggtacaag aacgtctgac gcagcaaatt
    11341 ctcattgcgc tacaaacgct gctgggcacc aataacgtgg ctgtctctat cgacgcggtg
    11401 cattactgcg tgaaggcgcg tggcatccgc gatgcaacca gtgccacgac aacgacctct
    11461 cttggtggat tgttcaaatc cagtcagaat acgcgccacg agtttctgcg cgctgtgcgt
    11521 catcacaact gataaaaagg caggaaccgt ggagcgcaac gtcacgctcg attttgttcg
    11581 cggcgtcgcc attctgggga tcctgctatt aaacatcagc gcctttgggt taccaaaggc
    11641 ggcttatctc aatcccgcct ggtacggcgc tattacgcca caggatgcat ggacctgggc
    11701 atttctcgat ctcatcggcc aggtgaaatt cctcacgctt tttgcgctgt tgtttggtgc
    11761 gggcctgcaa atgttgctgc cccgtggcag acgatggatc cagtcgcggt taacgctgtt
    11821 agtcttgctg ggctttattc acggtttatt gttctgggac ggcgatattc tgctggctta
    11881 cgggctggtg ggcttaatct gctggcggct ggtgcgcgat gcgccatcgg taaaaagcct
    11941 gtttaatacc ggcgtcatgc tttatctggt ggggcttggc gttttgctgt tattggggtt
    12001 gatttccgat agccagacca gccgcgcctg gacacctgat gcatcggcta ttttgtatga
    12061 aaagcactgg aaacttcaca gtggtgttga cgcgatcagt aatcgtgccg atggtgttgg
    12121 caacagttta ctggcactgg gcgcacagta tggctggcaa ctggcaggga tgatgctcat
    12181 tggtgccgca ttgatgcgca gtggctggct gaaagggcag ttcagctttc gtcactatcg
    12241 tcgtactggt tttgtgctgg tggcgattgg agtgatcatt aaccttcctg ccatcgccct
    12301 gcaatggcga ctggactggg cgtatcgttg gtgcgccttc ttacttcaga tgccgcggga
    12361 actgagtgcg ccgtttcagg cgattggcta tgcgtcgctg ttttatggct tctggccgca
    12421 attaagccgc tttaagctgg tgctggcaat cgcctgtgtc ggacggatgg cactgaccaa
    12481 ctatctattg caaacgctga tttgtactac gcttttttac cacctcggtt tgtttatgca
    12541 gtttgaccgt ctggagctgc tggcgtttgt tattccggta tggttggcga atatcttctt
    12601 ctctgttatc tggctgcgtt acttccgcca ggggccggtg gaatggctct ggcgtcagtt
    12661 aactttgcgt gctgccggac cgacaatatc taaaacatca agataacgat ctggatcaca
    12721 ttcgttaaca aaacggctgt aaccgtttcc attgctgtga ctcgattcac gaagtcctgt
    12781 attcagtgct gacaaaatag ccgccagcga gcagtcattt actgcaatct cataacaggt
    12841 agtgaatatg atcaccattc gtgatgtagc gcgtcaggct ggcgtctctg tggcaacggt
    12901 ttcccgggtg ctcaataaca gcacgctggt cagtgccgat acgcgtgaag cagtaatgaa
    12961 agccgtgagt gagctggatt atcggccaaa cgccaatgcc caggcgctgg caactcaggt
    13021 tagcgatacc attggcgtgg tggtgatgga cgtttctgat gcgtttttcg gcgcgctggt
    13081 aaaagcggtg gatctggtcg cccagcagca tcagaaatac gtgctaatcg gcaatagcta
    13141 tcatgaagcg gaaaaagagc gtcacgccat tgaggtgtta attcgccagc gttgtaatgc
    13201 gttgattgtt cactcaaaag cattgagtga cgatgaactg gcacaattta tggataacat
    13261 tcccggtatg gtgttaatca accgcgttgt gccggggtac gcccatcgtt gcgtttgcct
    13321 ggataatctc agcggtgccc gaatggcgac gcgcatgttg ctgaataacg gtcatcaacg
    13381 tattggttat ctttcttcca gccacggcat tgaagatgac gccatgcgta aagcaggctg
    13441 gatgagtgcg ttgaaagagc aggatattat tccgccggaa agctggattg gcactggcac
    13501 gccggacatg ccgggcggtg aggcggcgat ggttgaactg ctggggcgca acctacaact
    13561 aaccgctgta tttgcttata acgacaatat ggccgctggc gcactgacag cattaaaaga
    13621 taatggcatt gcgattccgt tacatctctc aatcattggt ttcgatgata ttcccatcgc
    13681 ccgttacacc gacccgcaat taacgacggt gcgttatccc attgcttcaa tggctaaatt
    13741 agctaccgaa ctggccttgc agggggcagc agacaatatt gatcctcgtg ccagccactg
    13801 ttttatgccg acgttagtgc gtcgccattc tgtcgcaaca cgccagaatg cggcggcgat
    13861 cactaactca acaaatcagg cgatgtaacc gctttcaatc tgtgagtgat ttcacagtat
    13921 cttaacaatg tgatggctat gattgcaccg ttttaacgtt gtaaccagta tgtaacagtg
    13981 aataatcact ttcaccgagg taatagcgtc ataacaacaa ttaaagccgt tttctggagc
    14041 gttaccgggc atggaagaac gaattttaaa aagtgagctt cggcgttcag taacacttca
    14101 ttaactctac tgccccgccg agcatttatc tcaagcacta ccctgcataa gaaaaaccgg
    14161 agataccatg aataagaagg tgttaaccct gtctgctgtg atggccagca tgttattcgg
    14221 tgccgctgca cacgctgctg atactcgcat tggtgtaaca atctataagt acgacgataa
    14281 ctttatgtct gtagtgcgca aggctattga gcaagatgcg aaagccgcgc cagatgttca
    14341 gctgctgatg aatgattctc agaatgacca gtccaagcag aacgatcaga tcgacgtatt
    14401 gctggcgaaa ggggtgaagg cactggcaat caacctggtt gacccggcag ctgcgggtac
    14461 ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt ttcttcaaca aagaaccgtc
    14521 tcgtaaggcg ctggatagct acgacaaagc ctactacgtt ggcactgact ccaaagagtc
    14581 cggcattatt cagggcgatt tgattgctaa acactgggcg gcgaatcagg gttgggatct
    14641 gaacaaagac ggtcagattc aattcgtact gctgaaaggt gagccggggc atccggatgc
    14701 agaagcacgt accacttacg tgattaaaga attgaacgac aaaggcatta aaactgaaca
    14761 gttacagtta gataccgcta tgtgggatac cgctcaggcg aaagataaga tggacgcctg
    14821 gctgtctggc ccgaacgcca acaaaatcga ggtggttatc gccaacaacg acgcgatggc
    14881 aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc agcattccgg tgtttggcgt
    14941 cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt gcactggcgg gcaccgtact
    15001 gaacgatgct aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg
    15061 caaaggtgcg gctgatggca ccaactggaa aatcgacaac aaagtggtcc gcgtacctta
    15121 tgttggcgta gataaagaca acctggctga attcagcaag aaataagact gattgggcgc
    15181 agtcgtttac tgcgccctta ttgtatggcc aacaaggtaa aattatatgg tcagctcaac
    15241 gactccgtct tccggggaat acttgttgga aatgagcggt atcaacaagt cttttcctgg
    15301 tgttaaggca cttgataacg ttaatttaaa agtccgacca cattctatcc atgcattaat
    15361 gggggaaaac ggtgcaggaa aatcgacatt attaaaatgc ctgtttggta tttatcaaaa
    15421 agactccggc accattttat tccagggtaa agagatcgat ttccattccg ccaaagaagc
    15481 actggaaaat ggtatttcga tggtacacca ggagttaaac ctggtattac aacgttcggt
    15541 gatggacaac atgtggctgg ggcgatatcc caccaaaggc atgtttgtcg atcaggacaa
    15601 aatgtaccgc gaaaccaaag cgatttttga tgaactggat attgatatcg atccgcgtgc
    15661 acgcgtcggc acattatccg tttcgcaaat gcagatgatc gaaattgcca aagcgttttc
    15721 ctataacgcg aaaattgtga ttatggatga accgacttct tcgttaaccg aaaaagaggt
    15781 caatcatctg ttcactatta ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat
    15841 ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg
    15901 tcagtggatc gctaccgaac cgctggcagg gctgacgatg gacaagatca tcgccatgat
    15961 ggttgggcgt tctcttaacc agcgtttccc tgacaaagaa aacaagccgg gcgaagtgat
    16021 tctggaagtg cgtaacctga cgtcgctgcg ccagccgtcg attcgcgatg tctcgtttga
    16081 tctgcataaa ggggagattc tcggtatagc cggtctggtg ggagcgaaac gtaccgatat
    16141 tgttgagacg ttatttggta ttcgcgagaa atcggctggc accattacgt tgcacggcaa
    16201 aaagatcaat aaccataatg ccaacgaagc cataaaccac ggatttgcac tggtaactga
    16261 agagcgccgc tcaacgggaa tttatgccta tctggatatt ggctttaact cgttaatttc
    16321 caatatccgc aactataaaa ataaagttgg tttactggat aactcgcgga tgaaaagcga
    16381 tacccagtgg gtcattgatt cgatgcgggt aaaaacgcca ggtcatcgga cgcaaatagg
    16441 ttcgctctcc ggtggtaatc agcaaaaagt gattattggt cgctggctgt taacgcaacc
    16501 agaaatatta atgctcgatg aaccgacgcg cggtattgat gttggggcta agtttgagat
    16561 ttatcagtta attgccgaac tggcgaagaa aggcaagggg attattatta tctcctccga
    16621 aatgccagag ttgttaggga taacagaccg tattctggtc atgagcaatg gtctcgtttc
    16681 cggtattgtc gatacaaaaa caacaacgca aagcgaaatt ctgcgtcttg cgtctttgca
    16741 cctttaagat taggggcttc ccatgagtgc gttaaataag aaaagttttc ttacttacct
    16801 gaaagagggc ggtatttacg tcgttctttt agttttgctg gcgattatta ttttccagga
    16861 cccaacattt ttaagtctgt tgaacttaag taatattctc acccagtcat cggtgcgtat
    16921 tattatcgcg cttggtgtgg cagggttaat tgtcacccaa gggactgacc tttccgcagg
    16981 tcgccaggtg gggctggcgg cagtagtagc agcgacgtta ttgcagtcca tggataacgc
    17041 caacaaagtg ttcccggaaa tggcgacgat gccgattgcg ctggttattc tgattgtctg
    17101 tgccattggt gcggtgatcg gtttgatcaa cggcttgatt atcgcttatc tcaacgtgac
    17161 gccgttcatt accacgctcg gtacgatgat tatcgtctac ggcattaact cgctctatta
    17221 cgactttgtc ggggcgtcgc caatttccgg ttttgacagt ggtttctcca cttttgccca
    17281 aggctttatt gcgctgggga gtttccgtct ctcttacatc accttctatg cgttgattgc
    17341 ggtggtgttt gtctgggtgt tatggaacaa aacccgcttc ggtaagaaca tttttgccat
    17401 tggcggtaac ccggaagcgg cgaaagtatc tggtgtcaac gtcggcctga acctgctgat
    17461 gatttacgcg ttgtctggtg tgttctatgc cttcggcggg atgttagaag ccggacgtat
    17521 cggctcagcc accaacaacc tcggctttat gtatgagctg gatgctatcg cggcgtgcgt
    17581 ggtaggcggc gtatcgttca gcggcggtgt ggggacggta attggtgtgg tgaccggggt
    17641 aattattttt accgtcatta actatggcct gacgtatatc ggcgtaaacc cgtactggca
    17701 gtacatcatc aaaggggcga ttattatctt cgccgtggcg ctggattcac tgaaatacgc
    17761 gcgtaagaag tgatgtgaat gtagttcgca tccggtcaga aatgcacata acatcatgag
    17821 ttgtatggtt ttgtatgccg gataaggcgt ttacgccgca tccggcgaca ggaaccaaat
    17881 gccggatgca acgctggcgc gttttatccg gcctacaatg agcacaacat actgatttat
    17941 attgttttgt atgccgggta aggcgttcac gccgcatccg gcgacaggaa ctaaatgccg
    18001 gatgcgtcgc tggttttaca acgttaccgg gtgttctttc tcgcctttct taaacttcac
    18061 ttccccaaga tcaatacaac ctaccgggca gacatgacca caaagcagac aacccacgca
    18121 tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc cattccatcg cctggtgacc
    18181 gccgtcgtaa caggaaatat aacagcgtcc acagccaaca catttatcaa gattgatatg
    18241 gggatagaca atataactgc ggtctaaatc ttccgccggg acaatattgt gattcgccag
    18301 acctaccatc tcctgcaacg aatcaaaccc ttgatcggcg agataatgcg acaacccgct
    18361 tgccatatct tccactatcc gatacccgta ctgcataatg ccggtggtca cctgcaacgt
    18421 cgctgcaccg agcaataaaa actccgccgc atcttcccag gtttcaatgc cgccgatacc
    18481 gctgatcggg aaatcgcgca gttctggatg ggtgcgcatt tgctgaataa agcgtagggc
    18541 gatcggtttt accgctttac cggaatatcc ggaaatactc gatttaccgt taacgatcgg
    18601 cataccaatt ttctgattaa gatcgatatt ggtgatggat ttaacggtgt taatcgccgc
    18661 aatgccatct gcgccgccgc gcttcgccgc cagcgccact tcgcacatat caccaatatt
    18721 cggcgtcatc ttcgccagca ttggcagcgt ggaaccacgt tttactgccc gacaatattt
    18781 ttctaccagc tccgggcttt gcccgacatc gctacccatc gcatgagaag tcatttgcgg
    18841 acaggagaag ttacactcga tcatatccgc gccagcttct tgcaccagac gcgccagctc
    18901 ttcccattgc tgctcatttt cgcccatgat tgaagcgatc aatactttgt ccggataatc
    18961 ttctttcagc cgacgcaggg cggccagatt ctcttccaac ggatgttcag caatctgctc
    19021 catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa aacgcggcga
    19081 gacttcgttg gcgataaaaa aaccgatcgt tttaaacacc acgccgcccc aacctgtgtc
    19141 gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaaa ggcagaacgg
    19201 gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct
    19261 ccctctaaat agtgatgaat cgccccggcg gcttctttcc cggttttcac tgcatagacc
    19321 acggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcgggtc gcgggtctgg
    19381 taatgttgcg tttcgatggt gttacgctgc ggctctaact ccgcaaaggc atccagtctg
    19441 gcatgctgac cgacggcgag aataatttta tctgccgcca tcgttagttc acccggtaaa
    19501 cgaacatgct taaaggtgac tttattgcct tccacggcta ctggtgtaaa tccatcgatg
    19561 atcgaaacgc ccagctctct ggcgctggta aactcttttt cgcttgccgg aaactcatct
    19621 aactcttcac gcgctacgca ggttaccgcc tgacagccga gaactttcag cgtgctggct
    19681 acgtccatcg cgacatcacc gccgccgata attaatgcgc tttgtggaat gctgatatcg
    19741 ccttgcgcct gtcgtgcacg ttgcaggaag tcgacggcaa tctcaacgtc gctatgctca
    19801 aacagcgata acccggaacc gctcgataac cctacggtga ccagtaccgc gcggttttcc
    19861 gctttcagct gctccagggt aagtgtttta ccgatttcgt tattgcactt aatggtcacg
    19921 cccatttttt ctatacgggc gatttctgca tccagcaccg attgcggtaa ccggaattgc
    19981 ggaatgccgt tacgcaacca gccaccgggt tgtgcttctt tctcatagat cgtgacgtca
    20041 taaccctggt ttgtcagcgt cacactggcc tgcaatccgg caggacctgc gccaataatg
    20101 gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccagt ttgttgttca
    20161 aaatcagtaa caaaacgctg taaacgaccg atatcaatgg gagcatcaac accggcacgt
    20221 gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac ggcaccgagg
    20281 gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta gattgagcga
    20341 ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc gtcatgacat
    20401 aataaacagc gagaggcttc tttaatcgcc agtaaaggcg taaaagccgg agtgagttca
    20461 tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat taaggcgaac
    20521 gctaaataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt taaactgtga
    20581 aaggattcac aaatagctgc ggttcagaat gttatatccg cagcttattg agtgaagtaa
    20641 taattattct caataattaa cagaaaaggg aaaatattaa cgatagaaaa taagataaac
    20701 aatcatgccg atagataaaa ttccgaagcc gatatatgcc accatataag cgaagaagag
    20761 tccgggattt ttcgctcgcc agacatacac cggttctggt gcatttttaa cccgtttatc
    20821 aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa caaaatatcg gaataaggaa
    20881 aaaaacggca acgacaaaaa actgctgtac atccataact actttccttg tttcttttgt
    20941 aattcaagta actgttcggg tgtgacggcg ggatacccct gcgcatcttc cggcacctgg
    21001 tgcatagccg gaatagggac cagcggccct aaaaaacgcg gttcacgttt aaaaatataa
    21061 aggtcagcca gcgcaccgaa acgggcggca aattcgcgaa tacgtactga cagcatatct
    21121 ttcggtgacg gtacggcata acactgagcc tgaatcccca tatgcagcgc aataaataat
    21181 gcccgctcac agtggaaacg ttgggtgata ataatgaaat cattagtatc gaaaactttg
    21241 cgtgtacgca cgatggaatc cagcgtacga aagcctgcgt aatcgagaac aatatctgat
    21301 gggtcgacac cagctgcgat taaatctttg cgcatggtca tcggctcatt ataactttgc
    21361 aatgcgttat cgccgctcag taatagataa tttaccttac cgctgttata ggcattaatc
    21421 gctccttgaa tgcggtagcg ataatactga ttaattacgc cagtccgata atattttgct
    21481 gtgccgagca ccacaccgac ctggcggtag gggagatcct gcaattcgtc gtagatataa
    21541 ggcgcggttt tccagctcat ccagcgatcg aggccgagca cagtcaacag cagcaagccg
    21601 atcaggacta acaggctgag gaacacgcgc tttaacatgc aggagactcg atctaaggaa
    21661 aatttccatc aggctacttt acccgctggt tttgcgcaag aaacggcaaa ctgtttgccg
    21721 ggattttcga caccaggaaa gggctggcaa gccggacaag gcgttcacgc cgcatccggc
    21781 accaggctta agcgagaagc acgcggtcga tattgtgaca gccgagagct ttcagcgttg
    21841 cggaggtggc atcccactga atcaacggcg catcggcttt ttctgccaga accgcgcgct
    21901 ggatatccgg gtaatcataa cccttgagat tcaacagaat taacgccccc tgcaacggcg
    21961 gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc gctgaaaata cgaccgtctt
    22021 tacattccag cgcgacaccg cttggcgact tactgtaagg catgtgcgaa cggtttgccg
    22081 ccgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc atagccgtga tcctgctcgt
    22141 ccatcagcag cgttttaatc tccagatctt tcggcccaaa ggcatccggt agatagtcac
    22201 gcagcgcgtg tgcctcgcgt cccggaagat gaatacgcag atccagaccg ctgttcagtt
    22261 cattcataaa ctgacggcag tgaccacaag gcgtgtagtt aacggtgatg gctgcaagcg
    22321 ctttttcacc actcagccag gcgtggctga tcgcgctttg ttcggcatga acggtttgct
    22381 gcattgtcgc accaataaat tccatattgg caccgaaata ccaggttccg ctcacaccgc
    22441 gcgcaattgc gccaacatta aaattcgaca atggcgtacg cgcacaggct gccgccagcg
    22501 gaagtagtgc gaatgccagc gcgtcttcgt ccagccccgt tgcgctcttc agcgatgaga
    22561 cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc cagaataggt tccagtgcag
    22621 attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa acgtggatgc atggcgttgc
    22681 ctcataatca tgtaatggga taacagttta cgaacccgtt atggctttat atgtgatctg
    22741 aatctcatta attatgcaac gcgtgaaact tacatcaaaa atgcgcgacg catcgcaaat
    22801 tttagcccat tactgccaga ataatcggga aaaggaacgg tgcgatcagc gaagtaataa
    22861 tcccgcataa caccagcgcc agcgaactaa atgcaccttc ctgataatcc agctcggcgc
    22921 agcgcgccgt accgagggcg tgcgaggctg tgcccattgc cagaccgcgc gcagctttgg
    22981 tacgaatacg catcgcatta agcaatgcat ggccaaacac agcgccgagg atgccgacga
    23041 aaatcacgca aactgcgctg attgccggaa taccgccaat actgccgcca actgccattg
    23101 caataggcgt ggtgactgat ttcggcagga ttgacgcggc gatttccggt gaagcgccca
    23161 tcaacaatgc cacagaagtc cccgtcacca tcgcaaccac gctgccgata aaacagatgg
    23221 tgatgatcga tttccagcgc gcgcggatct ggtgtagctg ctcatataaa ggataggcca
    23281 gtgcgacgac cgccggttgc agcagatcgt ttagcacttc gctaccttta aagtagctgt
    23341 cgtaagagat gccagtcagc attaaaaaag gaatgatcac caccattgct accagcaacg
    23401 ggttaagcag gggaaactta taccgtgccg ccagtttgcg ggcggcaaaa aagacaatca
    23461 acgtcagcgg taatgaccac cagatattcg ccatcattct tctgatcctt tctgacctac
    23521 gactttacgt tcaccgtgca ccagttgtga actccagctc accaccagaa aaaccaccag
    23581 cgtactgact gcacaggaaa ccactaccgg accaaactgt gcgcggagca aatcaaaata
    23641 ttgcatgacg cctacgccaa tcggcacaaa caatagcgcc atatagcgaa tcagtacgta
    23701 gcaccccgga ttgacccatt tcgccggaag aatttgcaag gccagcagga caaacaggat
    23761 cagcatcccg atgatgctgc ccggaatggt taccggtagc agggaagcaa tgaaaatgcc
    23821 tgcatacagg caggcataaa tcaggacaaa agcacgtaaa tattgccaga taatgttcag
    23881 tgtcttactc ataatcaaca ccccagatga gatggattca tcatacaatt aattcgtaaa
    23941 acgtgctacc gatcacatta agaaatcagc gcttattttt tcgctccccg gtattacgca
    24001 atgcgcagcg aactcaggca tacatcaagc atagagaggg attgcagttt gcagcaatcc
    24061 ctctttgcag acctttaatc cgttgaccgg aggcgctaac atagcgcctc atttttttgc
    24121 gggtgatgat atgcgtgtgt tactggctcc gatggagggt gtacttgact ctctggtgcg
    24181 ggaattactg accgaagtta acgactacga tctgtgcatc accgagtttg tccgcgtggt
    24241 ggatcaactg ctgccagtaa aagtctttca tcgcatttgc cctgagctac aaaatgccag
    24301 ccggacacca tccggtacgc tggttcgcgt gcagttgtta gggcagttcc cgcagtggct
    24361 ggcagagaac gccgcccgtg cggtggagtt aggttcctgg ggcgtggatc ttaactgcgg
    24421 ctgcccgtcg aaaacggtta acggtagcgg tggtggggca acgttactga aagatcctga
    24481 actcatctac cagggtgcaa aagcgatgcg cgaagctgta ccggcgcatt tgcctgtcag
    24541 cgtaaaagtg cgtctgggct gggacagcgg tgagaagaaa tttgaaatcg ccgatgcggt
    24601 tcaacaggct ggtgctacgg agctggtagt acatgggcgg acaaaagagc agggttaccg
    24661 cgcggaacat attgactggc aggcgattgg cgagattcgc cagcggctta atattccggt
    24721 gattgccaac ggtgaaatct gggactggca gagtgcgcaa caatgcatgg cgatcagcgg
    24781 ctgcgacgca gtgatgattg gtcgcggggc gctcaatatt cccaacctga gccgtgtggt
    24841 gaaatataac gaaccgcgaa tgccgtggcc ggaggtggtt gctttgctgc aaaaatatac
    24901 ccgtctggaa aagcagggcg ataccgggtt atatcacgtt gcgcggatta aacagtggtt
    24961 gagttatttg cgtaaagaat acgatgaagc aacggaatta tttcagcatg ttcgggtgtt
    25021 gaataattcc cctgatattg caagggctat tcaggccatt gatatcgata agctgcgata
    25081 atagtggaat cgcagcttat ctgagacaat gaatcgctta ttcagcttca tcatcttgtt
    25141 gcgctccttt catgagctaa gccacatatt gccactggcg caaggagcgc gcagggggag
    25201 gacaatcgcc tcctccccct gcacccccgg gctctggcga acaaaatcgc cgctgcgcgg
    25261 taccttcagc ttatcccctt cggctaccgg gtcgggcgcg aggtaacgtc cttgtaagac
    25321 gcgccctcag cccacatcca tgtgggctgc cccggccttc ggggaacgct tcagcgattt
    25381 tgacgccacc aacaccggag ctgtttgcaa atcaagtaca caaaatatct agttcctgac
    25441 ctgacaactt atatgccata tctgattttt aaaatatcat cttaaatacg ccagtcacca
    25501 ccagtagccc aatcaggaaa ataatcaata cagcccagag tataattttc attggcgttc
    25561 ctttatttgt gagtgctgaa taagtatagg aaaaaacgaa gccctttgcc gctattcata
    25621 aacgattaag ataaatcggg agatatcatt cgcttagtgt atatttttct ggatatgtcg
    25681 ttttattgcc actgatgtat tgccacgcga aactaaaaaa tatatcgcaa cacacggtaa
    25741 tctccgcgca ccttttctta cctgattatt cccccatgaa tcgtgattcc ttttatcccg
    25801 ccatcgcctg ttttccgctg ttactgatgc tggccgggtg tgcgcctatg catgaaaccc
    25861 gccaggcgtt aagccagcac acgctcgctg tgcaagttga caccgcatta cccacggcac
    25921 tgaaaaatgg ttgaccagac agccaatggt ggctggagta tcacgatgat caactcactt
    25981 cattaattaa caatgcacta cagaacgcac cgtatatgca ggtggctgaa cagcgcattc
    26041 agctggctga agcccaggcg aaggcagttg ccactcagga tggtccgcag ctcgactttt
    26101 ctgcggatat ggagcggcaa aaaatgtcgg cagaagggtt aatggggccg tttgctttga
    26161 acgatccggc cgcaggtacg accggtccgt ggtacaccaa cggtactttt ggcttaacgg
    26221 cgggctggca tctcgatatc tggggaaaga atcgggcgga ggttactgct cgactgggta
    26281 cggttaaagc acgggcggcg gaacgcgagc aaacccgcca attgctggct ggcagcgtag
    26341 cccgcctgta ctgggagtgg caaacccagg cggcgttaaa cacggtcttg cagcaaatcg
    26401 aaaaagagca gaacaccatt atcgcgaccg atcgccagct atatcagaac gggattactt
    26461 cttcagttga aggtgtggaa accgatatta atgccagcaa aacccggcag cagctcaacg
    26521 atgtcgcggg taaaatgaaa attattgagg cacggttaaa tgcactcaca aatcaccaga
    26581 caaagtcact gaagcttaaa ccggtcgcgt tgccgaaagt ggcaagccag cttcctgatg
    26641 aactggggta ctccttactg gcgcggcggg cagatttgca ggcggcgcac tggtacgttg
    26701 agtcatcgtt aagcactatt gatgcggcaa aagcggcatt ttatcctgac atcaacctga
    26761 tggcctttct gcaacaggat gcgttgcact taagcgatct gttccgtcat tccgcgcagc
    26821 aaatgggcgt tacggcaggc ctgacgctac ccattttcga tagtggtcgt cttaacgcca
    26881 acctcgatat cgctaaagcc gaaagcaact tgtctatcgc cagctacaac aaagcggtgg
    26941 ttgaagcggt gaatgacgtg gcgcgggcag ccagtcaggt tcagacactg gcggagaaaa
    27001 accagcatca ggcgcaaatt gagcgcgatg ccttgcgtgt agtaggtctt gcgcaggccc
    27061 gctttaacgc gggcctcatt gctggttccc gggtcagcga agccagaatc ccggcgctgc
    27121 gtgagcgggc caatggcctg ttattgcaag ggcagtggct ggatgcctcc attcaactca
    27181 ccggtgcgct gggcgggggg tacaaacgct gatgatatgc gccttctata cttaacattt
    27241 attcagcgtt aagtggagaa ctcgatggca caggtcgcga taattaccgc ctccgattcg
    27301 gggatcggca aagagtgcgc gttattactg gcgcagcagg ggtttgatat tggcattacc
    27361 tggcactcag atgaagaagg ggcaaaagat accgcgcgta aggtggttag ccacggcgta
    27421 cgtgcggaga tcgtgcaact ggatctcggc aatctaccag aaggtgcgca ggcgctggag
    27481 aaacttattc aacggctggg gcgtattgat gtgctggtga ataatgcggg cgcaatgacc
    27541 aaagcgccgt ttcttgatat ggctttcgat gagtggcgca agatttttac cgttgatgtc
    27601 gatggtgcat ttttatgctc gcaaattgct gcccgccaga tggtgaagca agggcagggc
    27661 ggtcgcatca tcaacattac atcggtacat gaacatacgc cgctgccgga tgccagcgcc
    27721 tacacagccg ctaaacatgc gctcggcggg ttaaccaaag caatggcgct ggagttggtc
    27781 aggcataaaa ttttggtgaa cgcagtcgca cctggggcga tcgccacgcc aatgaacggc
    27841 atggatggcg gcgacgtgaa gcctgacgcg gagccttcga ttcccttgcg acgttttggc
    27901 acaacgcatg agattgccag cctggtggta tggctttgtt cggagggcgc aaattacacc
    27961 accgggcagt cgttgatagt ggatggcggc tttatgttgg cgaatccgca gttcaaccca
    28021 gaatagcgca gtgaggtgcg tggttatttc attgggttag gccagaaaag acacgccagc
    28081 gtcgcatcag gcatggtgca ccggatgtcg gatgcggcgc gaacacctta tccagggcaa
    28141 tagttttacg cctgatgatc cggctttttc ttcccgcgcc gtttcagcca ccagcgcacg
    28201 cctaccacca gaaccacgac cagaatcaac cagacccagt gcttcaaatg ctggtcgaga
    28261 ttgtgcaacc acggcgcaat cacctgccca ccagcgtaac cgatagtggt aaaaatcagc
    28321 gcccaggcaa atgcgccgag aatattcagc ggcagaaaga ttttcggcgg cagctggctg
    28381 gcaccaatca gcgtcgggcc aatcacccga aagccataca taaaacgcgt accaatgaca
    28441 aacagatacg gatggcgttg gataagtttc tgcgcccgct caattttatc ctgatgtttc
    28501 gagaaacggc gtaacagctt gccgccaaag cgccgcccgc acagataaag cacctggtcg
    28561 ccaatcatgc cgccaagcgc cacagaaagt accaccagcg ggaactttaa tagcccctga
    28621 tgtgccgcaa cgcctcccag caaagtcacg gtttcacctt ccgccaggct accgatcacc
    28681 agcgcggcat aaccatattg tgagatgagt gtattgagat ccataggaca gtctgtctcc
    28741 ctttgcttgc ctgtaagcat acaccctgtg aatcattcac gctgaaaaag gacgtacatc
    28801 agacggtgca aattacatga gcgaacaaaa aataatcaca agctgactta tacttgaact
    28861 gtcaggttca tgaactcaca acaagggggc ggtatgagcc atgtctgggg actgttttcc
    28921 catcccgatc gtgaaatgca ggtgattaat cgcgaaaacg aaacgatttc tcatcactac
    28981 acccaccacg ttttgctgat ggcggcgatt ccggtgattt gcgccttcat tggcactaca
    29041 cagattggct ggaattttgg cgatggcact atcctgaagt tatcctggtt taccggactg
    29101 gcgctggctg tcttgtttta cggcgttatg ctggctgggg ttgcggtgat ggggcgggtc
    29161 atctggtgga tggcgcgtaa ttatccgcag cgtccgtcac ttgcgcactg catggtcttc
    29221 gcgggctacg ttgcaactcc gctgttttta agcggtctgg tggcgcttta cccgctggtc
    29281 tggttgtgtg cgctggtcgg cacggtggcg ctgttttaca ccggctacct gctgtatctc
    29341 ggtattccga gcttcctgaa tatcaacaaa gaggaaggac ttagcttctc cagttcgaca
    29401 ctggcgatag gcgtgctggt gctggaagtg ctgctcgccc ttaccgttat actatggggc
    29461 tacggttatc gtttgttcta gaaagttctt ttgcgctgca ttgctggcgt aaatgtcagc
    29521 aatgcagcat ttcctcacga ttcttctttg acgatcgcac tttttgctcg ttatgatggc
    29581 gtcgctagcc tcagtaaatc cttactccgg cggtgcgcaa cccgtgcgcg tgaaccacta
    29641 tctgaatgct catcatgccg aaatttcgag tttctttatt tagcctggcc ctgatgctgg
    29701 ctgtgccttt tgcaccgcag gccgttgcga aaacggtagc cgctaccacc gcgtcacaac
    29761 cggaaattgc ctccggtagc gcgatgattg ttgatctgaa taccaacaaa gtgatctatt
    29821 cgaaccaccc ggatctggtg cgtccgattg cgtctatcag caaattaatg accgcgatgg
    29881 ttgtgctgga tgcacgactg ccgctggatg aaaaactaaa agtggatatc agccagacgc
    29941 cggagatgaa aggggtctat tcgcgtgtca gactgaacag cgaaatcagc cgtaaagata
    30001 tgctgttgct ggcgctgatg tcttcagaaa accgcgcggc ggcaagtctt gcgcaccatt
    30061 atcccggtgg ttacaaagcc tttattaagg cgatgaacgc gaaagcgaaa tcgctcggaa
    30121 tgaacaacac gcgctttgtt gaaccaaccg gattgtcggt gcataacgtt tcaaccgccc
    30181 gtgacttaac caaactactc attgccagca aacaatatcc gttgatcggg cagttaagta
    30241 ctacccggga agatatggca accttctcta acccgacgta tacgctgccg ttccgcaata
    30301 ctaaccatct ggtgtatcgt gataactgga atattcagtt aaccaaaacc ggttttacca
    30361 atgcggcggg ccattgtctg gtgatgcgca cggttatcaa taataaaccg gtggcgctgg
    30421 tagtgatgga cgcgtttggc aaatataccc attttgccga tgccagccgc ctgcgtacct
    30481 ggattgaaac cggtaaagtg atgcctgttc cggcagcagc gttgagctat aaaaaacaaa
    30541 aagccgccca aatggcggcg gcggggcaga cggcacagaa cgattaaatg aagtacggat
    30601 ggcgatactc tgccatccgt aatttttatt tcacttcctg ccagtttttc cgcttactgg
    30661 ttttaccaat ccccggattc atactgttgg tcggatcgtt ctcgcgataa aacttctgca
    30721 acgtttccgg tgctttatac aaatgaccga cgttatgctc ggcagggtat tgcgcgccgc
    30781 gctgctgtag cagttccagc atctgttctt tcaacacatg cacatccacg ccttttttca
    30841 cgatgtaatc ctgatggaag acatagcaca taaaatggcc ataatagagc ttgtgcacca
    30901 gctggctgtc gatctccggc ggtaaatgct cataccattc ggtgtcgtta cgccgcagag
    30961 cgatatccag cgccagaatg tcttcgactt catcggaatg cacagcctga taacgaattg
    31021 ctgcgcccgc agcggcgaaa cggtgtaaaa acgctttgct gccttcctcc ggcgtacaga
    31081 caaagaaatc gccctcggcc tgtttgaaat aatccactag ccacgatttg gcttcaccga
    31141 cgccatcgcc cgccattttt aacagcagat gatgctcgta tttatcgcgc cagtttttca
    31201 tgcgcggcgg taaatggctg gggaacaggt gaccgaattt ttgcattgca cggtcggtaa
    31261 aatgcggacg gaagaatttc actttctcca gcatcgcatc ggtgcgtccc ttgagattaa
    31321 agaagaacgg catcttgtcg gtgcctaact tatcaatcat caggaaggtg tctttgccgt
    31381 atttttccgc aatatcgtag atatcccggt gcatatattc cccggcaacc ggcagatttt
    31441 cgaaattagc cagaatatga cggcggattt cggtcagcac ttccggctgg ttggtgccga
    31501 tataaaacac ctgctgattt ttttccgctt cgaaggtatc aagacgtacc gcaaagacgg
    31561 ccagcttccc tgcgcaacca gaagattcaa ataaccgatc cggatcagcg ttataacgtg
    31621 cgggcgtgtc ggcttcaata tcacgaacgc ggtggacata gtcataatcg tgggcgtgac
    31681 gaccatcgtg gcgcacatcg tcatctttga tgcgatcgtc atccagcttg ctaaggatct
    31741 gctccggcgt ttcgcccaga tcaatcccca gatggttcac cagcgtcagt ttgccgtctt
    31801 catttatacg cgcgaataac gacatttcgg tatacgccgg gccacgttgc accagcgagc
    31861 cgccggagtt gttacaaata ccgccgatga ccgatgcgcc tatacacgat gatccaatca
    31921 ctgagtgcgg ttcgcgtccc agcggtttga gggctttttc cagtgaatag agcgtggtgc
    31981 ccggataggc cagcacctgt tcgcccttac cgagtacgtg cagcttgtcg agacgcaggg
    32041 tgctgatgat cacgatatcg cgatcataat cgttaccgtt tggcgtcgat ccttcggtca
    32101 ggcctgtatt ggcagcctgc atcagaataa ttttgtcagc ggtgacgcag gctttcagca
    32161 cccgccacaa ttccagtagt gagccaggga aaacgacagc aagcgcgtcg ccctgaccag
    32221 aacggaagcc cttgcgatag cgggccgttt ttgcgggatc ggtgagcagg tgtgaatgac
    32281 cgaccagacg ggcaagttca ttcaaaaagg ctttattatc agttgttgtc atggaagaca
    32341 ttttccactc cttgtggtgg cgaaaaaata tcacaaacta gaatagcgcc ttgtatgaca
    32401 attcagcgag tgaatatttt gaaaattcag acaataaact gagcattctt tttctctctc
    32461 tatcatgctt atggcaaact acggtttttg caccattttc gctgccacgt cgggcaacaa
    32521 aggaagaaaa atccatatga aatggctatg ttcagtagga atcgcggtga gtctggccct
    32581 gcagccagca ctggcggatg atttattcgg caaccatcca ttaacgcccg aagcgcggga
    32641 tgcgttcgtc accgaactgc ttaagaaaat gacagttgat gagaaaattg gtcagctgcg
    32701 cttaatcagc gtcggcccgg ataacccgaa agaggcgatc cgcgagatga tcaaagacgg
    32761 tcaggttggg gcgattttca acaccgtaac ccgtcaggat atccgcgcca tgcaggatca
    32821 ggtgatggaa ttaagccgcc tgaaaattcc tcttttcttt gcttacgacg tgctgcacgg
    32881 tcagcgcacg gtgttcccga ttagtctcgg tctggcttcg tcttttaacc tcgatgcggt
    32941 gaaaacagtc gggcgtgtct ctgcttatga agcggcagat gatggcctga atatgacctg
    33001 ggcaccgatg gtcgatgtct cgcgcgatcc gcgctgggga cgtgcttccg aaggttttgg
    33061 cgaagatacg tatctcacct caataatggg caaaaccatg gtggaagcga tgcagggtaa
    33121 aagcccggca gatcgctact cggtgatgac cagcgtcaaa cactttgccg catacggcgc
    33181 ggtagaaggc ggtaaagagt acaacaccgt cgatatgagt ccgcagcgcc tgtttaacga
    33241 ctatatgccg ccgtacaaag cggggctgga tgcaggcagc ggtgcggtga tggtggcgct
    33301 gaactcgctg aacggcacgc cagccacctc cgactcctgg ctgctgaaag atgttctgcg
    33361 cgaccagtgg ggctttaaag gcatcaccgt ttccgatcac ggcgcaatca aagagctgat
    33421 taaacatggc acggcggcag atccagagga tgcggtacgc gtggcgctga agtccggaat
    33481 caacatgagc atgagcgacg agtattactc gaagtatctg cctgggttga tcaaatccgg
    33541 caaagtgacg atggaagagc tggacgatgc tgcccgccat gtactgaacg ttaaatatga
    33601 tatgggattg tttaacgatc catacagcca tttggggccg aaagagtctg acccggtgga
    33661 taccaatgcc gaaagccgcc tgcaccgcaa agaagcgcgt gaagtggcgc gcgaaagcct
    33721 ggtgttgctg aaaaatcgtc tcgaaacgct gccgctgaaa aaatctgcaa ccattgcggt
    33781 ggttggcccg ctggcagaca gcaagcgtga cgtgatggga agctggtcgg cggcaggtgt
    33841 cgccgatcaa tctgttactg tgctaacagg gattaaaaac gccgtcggtg aaaacggtaa
    33901 agtgctgtat gccaaagggg cgaacgttac cagtgacaaa ggcattatcg atttcctgaa
    33961 tcagtatgaa gaggcggtca aagttgatcc gcgttcgccg caagagatga ttgatgaagc
    34021 agtgcaaacc gcgaaacaat ctgatgtggt ggtggctgtg gtcggtgaag ctcaggggat
    34081 ggcgcacgaa gcttccagtc gtaccgatat cactattccg caaagccaac gtgacttgat
    34141 tgcggcgctg aaagccaccg gtaaaccgct ggtgctggtg ctgatgaacg ggcgtccgct
    34201 ggcgctggtg aaagaagatc agcaggctga tgcgattctg gaaacctggt ttgcggggac
    34261 tgaaggcggt aatgcaattg ccgatgtatt gtttggcgat tataacccgt ccggcaagct
    34321 gccgatgtcc ttcccgcgtt ctgtcgggca gatcccggtg tactacagcc atctgaatac
    34381 cgggcgtccg tataatgccg acaagccgaa caaatacact tcgcgttatt ttgatgaagc
    34441 taacggggcg ctttatccgt tcggctatgg tctgagctat accactttca ccgtctctga
    34501 tgtgaaactt tctgcgccga ccatgaagcg tgacggcaaa gtgacggcca gcgtgcaggt
    34561 gacgaacacc ggtaagcgcg aaggggcgac ggtagtgcag atgtacctgc aggatgtgac
    34621 ggcttccatg agtcgtccgg tgaagcagct taaaggcttt gagaaaatca ccctgaaacc
    34681 gggcgaaact cagaccgtca gcttcccaat cgatattgag gcgctgaagt tctggaatca
    34741 acagatgaaa tatgatgccg agcctggcaa gttcaatgtc ttcatcggca ctgattccgc
    34801 acgcgttaag aaaggcgagt ttgagttgtt gtaattttcg atgtttgtat gccggataag
    34861 gcgtttacgc cgcatccggc aagaaacgcc gggcaaaata tgccttttaa gtatttatct
    34921 gattctgcaa ctgagtccac ccagtcaggc taactccgcc attatcaact atgcttttct
    34981 cttaattcgc tgaaaacaac agtaaaagaa tgaggaaagc atcatgccac tctcaaaggt
    35041 ctgggcaggt tcactggttt tgttggcagc cgtgagcctg ccgctacagg cggcttcccc
    35101 cgttaaggtt ggttcaaaaa tcgatacgga aggcgcgctg ctcggcaata tcattttgca
    35161 ggtgctggaa agtcacggcg ttcctacggt aaataaagtg cagctgggaa cgactcccgt
    35221 agtgcgcggg gcgatcactt ctggcgagct ggatatctat ccggaatata ctggcaatgg
    35281 cgcgtttttc tttaaagatg aaaacgatac ggcatggaaa aacgcgcagc aaggctatga
    35341 gaaagtcaaa aaactcgatg cagagcaaaa caagttaatc tggctgacgc ccgcgcctgc
    35401 aaataacacc tggaccatcg ccgtgcgtca ggatgtggca gagaaaaaca aactcacttc
    35461 gcttgccgag ctgagtcgtt atctgaaaga gggtggtaac ttcaaactgg cagcctcggc
    35521 agagtttatt gaacgcgccg atgccttacc cgcgtttgaa aaagcctatg gctttaagct
    35581 cggtcaggat cagttgctgt cactggccgg tggtgacacg gcggtgacga tcaaagccgc
    35641 tgcccagcaa acttctggtg ttaatgccgc aatggcttac ggcaccgacg gcccggttgc
    35701 ggcgctgggg ctgcaaacct taagcgatcc gcaaggcgtg caacctatct acgcgcctgc
    35761 gccagtggtg cgtgagtcgg tgctgaaaga gtatccgcaa atagcacagt ggctacagcc
    35821 agtcttcgcc agcctcgatg aaaaaacatt gcagcagctg aatgccagca ttgcagtgga
    35881 aggactggat gccaaaaaag tggcagccga ctacctgaaa caaaaagggt ggacgaagta
    35941 atttcccgtg acttatttcc gtattaatcc tgttctggcg ctgctgctgt tgctgacggc
    36001 aatcgcagcg gcgctgccgt ttatcagtta cgcacctaat cgtttagttt cgggtgaggg
    36061 gcgtcatctc tggcagctgt ggccgcaaac tctctggatg ctggtgggcg ttggttgcgc
    36121 ctggctgaca gcctgtttta ttcccgctaa aaaaggcagc atttttgcac tcattctggc
    36181 gcaattcgtc ttcgtattgc tggtgtgggg agctggaaag gcggcgacac aactggcgca
    36241 aaatggcagt gcgctggcgc gtaccagcct cggcagtggt ttctggctgg ctgcggcgct
    36301 ggcactgctg gcctgtagcg atgccatccg ccgaatctcc acgcatccgc tgtggcgctg
    36361 gttgttgcat atgcagattg ccattgttcc gctgtggttg ctgtactccg gcacgcttaa
    36421 cgatctctcg ttaatgaaag aatacgccaa ccgtcaggat gtgtttgacg acgcgctggc
    36481 acaacatctg acgttgctgt ttggtgcggt gctgcctgcg ttagtgattg gtgtgccgtt
    36541 gggcatctgg tgctactttt ccaccgcgcg gcaaggggcg attttttctc tgctgaatgt
    36601 cattcagacc gtgccttcgg tagcgctctt tggcctgttg attgcgccgc ttgccgcgct
    36661 ggttacggcc tttccgtggc tgggaacgct cggcatagca ggaaccggaa tgacacccgc
    36721 tctgattgca ctggtgctct atgccctgtt accgctggtg cgcggcgtag tagtcggctt
    36781 gaaccagatc ccgcgcgatg tgctggagag cgccagagcg atggggatga gcgggacgca
    36841 gcgtttcctg catgttcagt taccgctggc gttaccggta tttctgcgca gcctgcgggt
    36901 ggtgatggtg caaaccgttg gcatggcggt gattgcggcg ttaatcggcg caggcggttt
    36961 tggtgcgctg gtttttcagg ggctgttaag cagtgccatt gatttagtgt tgctgggggt
    37021 gatcccggta attgttctgg cggtgcttac cgacgcgctg ttcgatttgc ttatcgcact
    37081 gctgaaggtg aaacgtaatg attgaattta gccatgtcag caaactgttc ggcgcacaaa
    37141 aagccgttaa cgatctcaat ctcaattttc aggaagggag tttttcggta ctgattggca
    37201 cgtcgggttc cggtaaatcc actaccctga aaatgattaa ccgcctggtg gagcatgaca
    37261 gcggggtgat ccgctttgcc ggagaagaaa ttcgctcgct gccagtgctg gagttgcgcc
    37321 gccggatggg ctatgccatt caatctattg gcctgtttcc ccactggagc gtggcgcaaa
    37381 atattgccac cgtgccgcaa ttacaaaaat ggtcacgggc gcggatcgac gatcgtatcg
    37441 acgaattaat ggcgctactg gggctggagc caaatttgcg tgagcgttat ccgcatcagc
    37501 tttccggtgg tcagcagcaa cgtgtgggag tggcgcgcgc gctggctgcc gatccgcaag
    37561 tcttgctgat ggatgaacca ttcggcgcgc tggacccggt aacgcgtggc gcgttgcaac
    37621 aagagatgac gcgcattcac cgtttgcagg ggcgcactat tgtgctggtc actcatgata
    37681 ttgatgaggc gctacggctg gcagaacatc ttgtattgat ggatcacggt gaagtggtgc
    37741 agcagggtaa tccgctgacg atgctgactc gtccggcgaa tgattttgtc cgccagtttt
    37801 ttggtcgtag tgagctgggt gtacgcctgc tttcgttacg tagcgtggca gattacgtgc
    37861 gccgtgaaga acgggctgaa ggtgaggcac tggaagaaag gatgacgcta cgcgatgcgc
    37921 tttctctgtt tgtcgcgcgg ggatgcgagg tgctgccggt ggtgaacacg cagggcgagc
    37981 cttgcggcac gctgcatttt caggatctgc tggaggaggc gtaatcgtat gaagatgttg
    38041 cgcgatccgc tgttctggct gattgctctg tttgtggcgc tgattttctg gctgccttac
    38101 agccagccgc tgtttgctgc cttgttccca caactgccac gacccgttta tcagcaggaa
    38161 agctttgcag ctctggcact ggctcatttc tggctggtgg gaatttcgag tttgtttgcg
    38221 gtgatcattg gtactggtgc cggaattgct gtcactcgcc cgtggggtgc ggaatttcgc
    38281 ccactggtgg aaactattgc cgccgttggg cagacttttc cgcccgtcgc ggtgctggcg
    38341 attgccgttc cggtgatcgg ctttggtctg aaaccagcga ttatcgcctt gatcctttac
    38401 ggcgtgctgc ccgtcctgca ggcgacactt gccgggctgg gagcgattga tgccagcgtg
    38461 acagaagttg cgaaaggtat gggaatgagt cgtggtcagc gagtgcgtaa ggtcgagcta
    38521 ccgctggcag ctccggtgat tctggcgggt gtacgaactt cggtgattat caacattggc
    38581 acggcgacga tcgcctcaac ggtaggggcc aacacgctgg gtacgccgat catcatcggg
    38641 cttagcggat ttaataccgc gtatgtgatc cagggagcgt tactggtggc actggcggcg
    38701 atcatcgcag accgcctgtt tgaaaggctg gtgcaggcgt tcagccagca cgcaaaataa
    38761 aggtataacc tgcgagcatg acgccaccaa ttccgcctaa cgccataaac aggaacaggg
    38821 cgatgacccc aattttagct atgcgcataa tgcactcctt atgttaacga aaggattgta
    38881 cagtaaagcg tatttgttaa cgaatcatta aatgccgagt gggaaaatat catggccttg
    38941 ttcctgccaa ctggtgagtt gctgctgttg ggcggaggtt cgattatcac cgcaccatac
    39001 cagcaatgta cggccttcga acagttcagg gcgtagttga ttgagcgagt gggcgaggac
    39061 atcaatgcgc catccttgtt gactggcaat ccagccctcc agccacagac gagtggtatc
    39121 ctgaatattc cagccaacca ccagtgcatc tttaccctgt tttttgcgcg ccgaagccag
    39181 gcaaatagag atgtagttga tcagtacgcc gtcgaggatc gccagcagcg cctggagagt
    39241 cggttgttgg cactgaagcc gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg
    39301 ggcggggtaa tcctgaccgc gctctttgat ccacgttcgc aggctatgca gattgccgct
    39361 ttgcaggtaa gtcagtaatg tttcttgctg atcgcgccag ccgttctgca catcaacatt
    39421 ttcattactg agcagcattt ttactttgct gacctgcacg ccgttgtcga tccagcgttt
    39481 gatctcgcgg atccggtcaa tatcggcatc gttgaacagc cgatgaccgc cgtctgtccg
    39541 ttgcggtttc agcaatccgt aacgcctctg ccacgcgcgt aacgtgacag ggttaatatc
    39601 acaaagcaac gccacttcac caattgtgta aagcgccatc gtctcaccct tgctcgcgag
    39661 gtcccggttt aactttagac gcagttttgc gaaccaggta gttttgcccg ttttttgagc
    39721 atctataggg tgattttatt tttgccaggc gattttgagt gatcgtactc acgaattctc
    39781 atttttctgc aagagttcaa agaaagttaa acgcaagcaa tgtatgttac gcgttttaaa
    39841 gggaagtgtg gtttgcgggt atgtacgatt ttaatctggt gttgctgctg cttcagcaga
    39901 tgtgcgtttt tttagtcatt gcgtggttaa tgagtaaaac gccattattc ataccgttaa
    39961 tgcaggtcac ggttcgtctg ccgcataaat ttctctgcta catcgtcttt tccatcttct
    40021 gcatcatggg cacctggttt gggttgcaca ttgacgattc tattgccaat acccgtgcga
    40081 taggcgcggt aatgggcggc ttactcggcg gtccggtcgt cggtgggctg gttggtctga
    40141 ccggcgggtt acatcgatat tcgatggggg gcatgaccgc gctaagttgc atgatctcga
    40201 ccatcgttga aggattgctc ggcggcctgg tacacagcat ccttatccgt cgcgggcgca
    40261 ctgataaagt ctttaacccc attaccgccg gtgccgtcac gttcgtcgct gaaatggtgc
    40321 aaatgttgat catcctggcg atcgcccgac cttatgaaga tgcggtgcgt ctggtgagta
    40381 atattgctgc gccaatgatg gtcaccaata ccgtcggcgc ggcgttgttt atgcgtatat
    40441 tgctcgataa acgcgcgatg tttgaaaaat acacttcggc tttttctgcc actgcgctga
    40501 aagtggcggc ctcgacggaa ggcattttgc gccaggggtt taacgaagtg aacagcatga
    40561 aagtggcaca ggtgctgtat caggagctgg atattggtgc agtcgcgatt accgatcgag
    40621 agaaattgct ggcctttacc ggaattggtg gcgaccacca tttacccggc aaaccgattt
    40681 cttcgactta taccttaaaa gcgattgaaa ccggtgaagt ggtctacgct gatggcaacg
    40741 aagtacctta tcgttgctct ttgcatccgc aatgcaaact ggggtcgacg ctggtcattc
    40801 cgttgcgtgg tgaaaatcag cgagtgatgg gtaccatcaa attgtatgag gccaaaaacc
    40861 gtttattcag ttcaatcaac cgcacgctgg gcgaggggat tgcgcaactg ctttcggcgc
    40921 agatcctcgc cgggcaatat gagcggcaaa aagcgatgct cacccagtca gagatcaaac
    40981 tgcttcacgc ccaggtgaat ccccattttt tgtttaatgc gcttaacacc attaaagcgg
    41041 tgatccgccg cgacagcgaa caggccagcc agctggtgca gtatctttct acttttttcc
    41101 gcaaaaactt aaagcggcct tcggagtttg ttactctcgc cgacgaaatt gaacatgtga
    41161 acgcttatct gcaaattgaa aaggcgcgct tccagtcgcg gttgcaggtc aacattgcta
    41221 ttccgcaaga attatcccag cagcaattgc ccgcgtttac cctgcaaccg atagtggaaa
    41281 acgccattaa acatgggaca tcacaactgc tggatacagg gcgagtggca atcagcgccc
    41341 gacgtgaggg gcaacatttg atgctggaga tcgaagacaa tgccggtttg tatcaaccgg
    41401 taaccaatgc cagtgggctg gggatgaatc tggtggataa gcgtttacgt gaacggtttg
    41461 gcgatgacta tgggataagc gtcgcctgtg agcctgatag ttacacccga ataacgttac
    41521 gactaccatg gagggacgag gcatgattaa agtcttaatt gtcgatgatg aaccgcttgc
    41581 acgggagaac ctgcgcgtat ttttgcagga gcagagcgat attgaaatcg ttggagagtg
    41641 ttcaaacgcc gtagaaggga tcggcgcggt gcataaactg cgcccggatg tgctgtttct
    41701 cgatatccag atgccgcgca tcagtggtct ggaaatggtg gggatgctcg acccggaaca
    41761 tcgcccgtat attgtttttc tcaccgcgtt tgacgaatac gccattaaag cctttgaaga
    41821 acatgccttt gattatctgc tgaagccaat tgatgaagcg cgactggaga aaacgctggc
    41881 gcgattgcgt caggagcgca gcaagcagga tgtttcgttg ttaccggaaa atcaacaggc
    41941 gctgaaattt atcccttgta cggggcatag tcggatttat ttgctgcaaa tgaaagatgt
    42001 ggcatttgtc agcagtcgga tgagcggtgt ctacgttacc agtcacgaag ggaaagaggg
    42061 ctttaccgaa ctgacattac gtaccctgga aagtcgtaca ccactactgc gctgccatcg
    42121 tcagtatctg gttaacctcg cgcatttaca ggagatccgt ctggaagata acggccaggc
    42181 cgagttgatt ttgcgtaatg gcttaaccgt gccggtcagc cgccgttatc tgaaaagctt
    42241 aaaagaggcg attggcctgt aaaagactgc taaaatggct ttttgcctca tcaacacctg
    42301 aaggcctcat gctaagtaac gatattctgc gcagcgtgcg ctacattttg aaagccaata
    42361 ataatgacct ggtgcgtatt ctggcgctgg gtaatgtcga agccaccgcg gaacagatcg
    42421 ccgtctggct acgtaaagaa gacgaagagg gttttcagcg ttgtccggac attgttttgt
    42481 cttcattcct caatggcctg atttatgaaa aacgcggcaa ggatgagtct gctccggcac
    42541 tggagccgga acgtcgcatt aataacaaca tcgtgctgaa aaaattacgc atcgcgtttt
    42601 cgcttaaaac cgatgacatt ctggcaatcc ttaccgaaca gcagttccgc gtttcgatgc
    42661 cggaaattac ggcgatgatg cgtgcaccgg atcataaaaa cttccgcgaa tgcggcgatc
    42721 aatttttacg ttattttctg cgtggactgg cagcgcgcca gcatgtgaag aaaa
//
LOCUS       DAZFRG010000008        41937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFRG010000008 DAZFRG010000000
VERSION     DAZFRG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.008"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..394)
                     /locus_tag="ACHKUC_000305"
     CDS             complement(<1..394)
                     /locus_tag="ACHKUC_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616635.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEVLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASVSTKGI"
     gene            complement(517..969)
                     /gene="tabA"
                     /locus_tag="ACHKUC_000306"
     CDS             complement(517..969)
                     /gene="tabA"
                     /locus_tag="ACHKUC_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO6616636.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKTLCAVGAPARVRKAVVKMLMA"
     gene            complement(1114..1707)
                     /gene="bdcR"
                     /locus_tag="ACHKUC_000307"
     CDS             complement(1114..1707)
                     /gene="bdcR"
                     /locus_tag="ACHKUC_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6616637.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            1778..2491
                     /gene="bdcA"
                     /locus_tag="ACHKUC_000308"
     CDS             1778..2491
                     /gene="bdcA"
                     /locus_tag="ACHKUC_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6616638.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2622..3017
                     /gene="yjgH"
                     /locus_tag="ACHKUC_000309"
     CDS             2622..3017
                     /gene="yjgH"
                     /locus_tag="ACHKUC_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6616639.1"
                     /translation="MVERTAVFLAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3299..3433
                     /gene="pyrL"
                     /locus_tag="ACHKUC_000310"
     CDS             3299..3433
                     /gene="pyrL"
                     /locus_tag="ACHKUC_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO6616640.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3437..4372
                     /gene="pyrB"
                     /locus_tag="ACHKUC_000311"
     CDS             3437..4372
                     /gene="pyrB"
                     /locus_tag="ACHKUC_000311"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO6616641.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4385..4846
                     /gene="pyrI"
                     /locus_tag="ACHKUC_000312"
     CDS             4385..4846
                     /gene="pyrI"
                     /locus_tag="ACHKUC_000312"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO6616642.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4919..5305
                     /gene="ridA"
                     /locus_tag="ACHKUC_000313"
     CDS             4919..5305
                     /gene="ridA"
                     /locus_tag="ACHKUC_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO6616643.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5511..8207)
                     /gene="mgtA"
                     /locus_tag="ACHKUC_000314"
     CDS             complement(5511..8207)
                     /gene="mgtA"
                     /locus_tag="ACHKUC_000314"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO6616644.1"
                     /translation="MFKEIFTRLTRHLPSRLVHRDPLPGVQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLHQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVATKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8348..8401)
                     /gene="mgtL"
                     /locus_tag="ACHKUC_000315"
     CDS             complement(8348..8401)
                     /gene="mgtL"
                     /locus_tag="ACHKUC_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO6616645.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8583..9533
                     /gene="treR"
                     /locus_tag="ACHKUC_000316"
                     /pseudo
     CDS             8583..9533
                     /gene="treR"
                     /locus_tag="ACHKUC_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            9652..11073
                     /gene="treB"
                     /locus_tag="ACHKUC_000317"
     CDS             9652..11073
                     /gene="treB"
                     /locus_tag="ACHKUC_000317"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO6616646.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11123..12778
                     /gene="treC"
                     /locus_tag="ACHKUC_000318"
     CDS             11123..12778
                     /gene="treC"
                     /locus_tag="ACHKUC_000318"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO6616647.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGKMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13172..15310
                     /gene="nrdD"
                     /locus_tag="ACHKUC_000319"
     CDS             13172..15310
                     /gene="nrdD"
                     /locus_tag="ACHKUC_000319"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO6616648.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15468..15932
                     /gene="nrdG"
                     /locus_tag="ACHKUC_000320"
     CDS             15468..15932
                     /gene="nrdG"
                     /locus_tag="ACHKUC_000320"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO6616649.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15977..16363)
                     /gene="cybC"
                     /locus_tag="ACHKUC_000321"
     CDS             complement(15977..16363)
                     /gene="cybC"
                     /locus_tag="ACHKUC_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6616650.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16419..17771)
                     /gene="pmbA"
                     /locus_tag="ACHKUC_000322"
     CDS             complement(16419..17771)
                     /gene="pmbA"
                     /locus_tag="ACHKUC_000322"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO6616651.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17865..18416
                     /gene="yjgA"
                     /locus_tag="ACHKUC_000323"
     CDS             17865..18416
                     /gene="yjgA"
                     /locus_tag="ACHKUC_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO6616652.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRKLAENEG"
     gene            complement(18572..19945)
                     /gene="mpl"
                     /locus_tag="ACHKUC_000324"
     CDS             complement(18572..19945)
                     /gene="mpl"
                     /locus_tag="ACHKUC_000324"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO6616653.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPVDAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20121..21119
                     /gene="fbp"
                     /locus_tag="ACHKUC_000325"
     CDS             20121..21119
                     /gene="fbp"
                     /locus_tag="ACHKUC_000325"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO6616654.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21152..22147)
                     /gene="yjfF"
                     /locus_tag="ACHKUC_000326"
     CDS             complement(21152..22147)
                     /gene="yjfF"
                     /locus_tag="ACHKUC_000326"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO6616655.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22134..23156)
                     /gene="ytfT"
                     /locus_tag="ACHKUC_000327"
     CDS             complement(22134..23156)
                     /gene="ytfT"
                     /locus_tag="ACHKUC_000327"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO6616656.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAELLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23170..24672)
                     /gene="ytfR"
                     /locus_tag="ACHKUC_000328"
     CDS             complement(23170..24672)
                     /gene="ytfR"
                     /locus_tag="ACHKUC_000328"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO6616657.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMRGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(24812..25768)
                     /gene="ytfQ"
                     /locus_tag="ACHKUC_000329"
     CDS             complement(24812..25768)
                     /gene="ytfQ"
                     /locus_tag="ACHKUC_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO6616658.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26078..26608
                     /gene="ppa"
                     /locus_tag="ACHKUC_000330"
     CDS             26078..26608
                     /gene="ppa"
                     /locus_tag="ACHKUC_000330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO6616659.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26988..27329)
                     /gene="ytfP"
                     /locus_tag="ACHKUC_000331"
     CDS             complement(26988..27329)
                     /gene="ytfP"
                     /locus_tag="ACHKUC_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO6616660.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27332..31111)
                     /gene="tamB"
                     /locus_tag="ACHKUC_000332"
     CDS             complement(27332..31111)
                     /gene="tamB"
                     /locus_tag="ACHKUC_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO6616661.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLDCLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGSVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31108..32841)
                     /gene="tamA"
                     /locus_tag="ACHKUC_000333"
     CDS             complement(31108..32841)
                     /gene="tamA"
                     /locus_tag="ACHKUC_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO6616662.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33047..33685
                     /gene="msrA"
                     /locus_tag="ACHKUC_000334"
     CDS             33047..33685
                     /gene="msrA"
                     /locus_tag="ACHKUC_000334"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO6616663.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34008..35351
                     /gene="paeA"
                     /locus_tag="ACHKUC_000335"
     CDS             34008..35351
                     /gene="paeA"
                     /locus_tag="ACHKUC_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO6616664.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35413..35619)
                     /gene="ytfK"
                     /locus_tag="ACHKUC_000336"
     CDS             complement(35413..35619)
                     /gene="ytfK"
                     /locus_tag="ACHKUC_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO6616665.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35944..36501
                     /gene="ytfJ"
                     /locus_tag="ACHKUC_000337"
     CDS             35944..36501
                     /gene="ytfJ"
                     /locus_tag="ACHKUC_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6616666.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(36491..37231)
                     /gene="cysQ"
                     /locus_tag="ACHKUC_000338"
     CDS             complement(36491..37231)
                     /gene="cysQ"
                     /locus_tag="ACHKUC_000338"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO6616667.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37421..39364
                     /gene="cpdB"
                     /locus_tag="ACHKUC_000339"
                     /pseudo
     CDS             37421..39364
                     /gene="cpdB"
                     /locus_tag="ACHKUC_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(39487..39867)
                     /gene="ytfH"
                     /locus_tag="ACHKUC_000340"
     CDS             complement(39487..39867)
                     /gene="ytfH"
                     /locus_tag="ACHKUC_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO6616668.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            39956..40816
                     /gene="qorB"
                     /locus_tag="ACHKUC_000341"
     CDS             39956..40816
                     /gene="qorB"
                     /locus_tag="ACHKUC_000341"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6616669.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            40924..41889
                     /gene="ytfF"
                     /locus_tag="ACHKUC_000342"
     CDS             40924..41889
                     /gene="ytfF"
                     /locus_tag="ACHKUC_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6616670.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
BASE COUNT        10126 a        11216 c        10543 g        10052 t
ORIGIN      
        1 gaatcccttt agtagaaact gaggcaagtg taatttctat cttcacctta gataaatccg
       61 ctcgctcccc ttcaggatta ttcatgtaaa acttcatttt ttcattgcca tcagtatctt
      121 tagaataaga tgaggactca atatgagcca tcatatttct acccgcttcg ttggagcgtt
      181 tgctaaaatc cacataaccc aaggtaaata tctttgccaa aaactccaaa aatgctctag
      241 gagaaagaat attgcttctg ctattctgcc ccccggcaat tcgggcaaaa tcattcattc
      301 ccacatacca ggtatctaag acttcatttt tttgtttaaa attgtcggct aatgtaatgt
      361 gttgagaata attcaaatct gatattttgc tcatgacgaa ctccttgagt taataagaac
      421 gtctaagcat tacaaatttt actcacaaaa aaatatcaaa aaacgctctt ttcacaaact
      481 cgccgtaaac ctggagttat ttacggcgaa aagtcatcac gccatcagca tcttcactac
      541 tgctttgcga acccgggctg gcgcgcccac tgcgcacagc gttttatgca cttcccccgg
      601 ataaaacaca acaaaatcac cttcattaag gataactgtt ttctcatcaa cgccttccgg
      661 caaaaatgcg atgtctttat cagctaacca gtcggtatcc ggggtgcctg caggttgcgt
      721 gctgaaggtc atgccttcct gaccttttaa cacaatctga atgtcgagat agcgggcatg
      781 gtactccgca cggcgagctt cgtgcggctc ggtcatatct tccgagataa gataaaacag
      841 acgattgcct tcgatatcgt gcttgccctt tggcgtttct gccgtaacgt gtgctttgat
      901 atgctcaatc gcctggcgta actcctgggg tagccacggc tgaagattat gaatatttcc
      961 gatgatcatc ttttccactc cttaattaaa acaacgtttc atttctatcg ttatacgaaa
     1021 agatgatcaa ttccgctagc cttaacgcgt gaattattgt cttatcacaa cttatcggca
     1081 ggcgcgttcc cgcctttcgg gtcaaggccc gcatcactcc ttgagaatag tttttatcgc
     1141 ttcccccgcc agtgcagctg ttgcacagag ttgttcgatg gagtgcccct cccgtgcctt
     1201 cgcagaaagc cctgacatca cggtactcat aaaatcagtc acacattgtg cgctttgtgg
     1261 atgccgcctg gcgatatagt cataaatggc cgtttctgcg gcgtgataat attgaacggc
     1321 aatatcacgc gcttgtggat catgactatg aatgccttca agaaccatac agccagcgca
     1381 gccgccgttt tggctatatc tgcgcgccgc ttcttttaat acctcagcca ggcactcccc
     1441 taccggacga tcatcacgaa gaatatcggc aagcggaata gcttccgtac cgacatattc
     1501 attgagtaca cggctaaata acccagcctt attgccaaaa gccgcgtaga ggctcggggg
     1561 gttaatacca agataatcag taacttcagc aacactgaca gcatcgaaac ctttttggtg
     1621 aaaaagcact tttgccgcag agactgcctg ctcaggagcg aaacgtctgg gacgacctgg
     1681 aacgcgagat tgttttttag tgaccattac aaaacttatt gacagaaagt taaaacagtt
     1741 ttgtaatgca tgttacataa taaatcaagg agtccttatg ggcgctttta caggtaagac
     1801 agttctcatc ctcggtggta gccgtggaat cggtgccgct atcgtacgtc gtttcgtcac
     1861 cgatggggcc aatgtacgat tcacctatgc ggggtcgaaa gatgccgctg aacgcctggc
     1921 acaagagact ggagcgacag cagtattcac agatagtgct gacagagacg ctgtcattga
     1981 tgtcgttcgt aagagcggcg cattggatat cctggtggta aatgcaggta ttggcgtctt
     2041 tggcgatgcc ctggaattga atgccgacga tattgatcgc cttttcaaaa tcaatattca
     2101 tgctccttat catgcctctg ttgaagccgc ccggcagatg cccgaaggcg ggcgcatctt
     2161 aatcatcggc tccgtgaatg gcgatcgtat gcctgttgca ggcatggctg cttatgccgc
     2221 cagtaaatct gccctgcaag gcatggcgcg cgggctggcc cgtgatttcg gaccgcgtgg
     2281 gatcaccatc aacgtcgtcc agccagggcc aattgatacc gacgctaatc ccgccaacgg
     2341 gccaatgcgc gatatgttgc atagttttat ggctatcaaa agacatgggc aaccggaaga
     2401 ggtcgctggt atggtcgcat ggttagcagg gccagaagcc agttttgtta ccggcgcgat
     2461 gcataccatt gatggcgcgt ttggcgcata accgactacg ctcaattaag cccagccatt
     2521 tcccatgatg tctgggtttt gtttactcac gtcgtccgct aaaagcggct cctggtaaat
     2581 ataaatcttc tgttcatgtc gtaaatcttg aggataacca aatggtagaa agaaccgctg
     2641 ttttccttgc tggccgacat tcactgtatg ctgaacatcg ttattctgcg gctattcgtt
     2701 ccggcgattt gctgtttgtt tccgggcaag taggaagtcg agaggacgga acaccagaac
     2761 ccgattttca gcaacaagtc agactggcat ttgataattt gcatgcgacc ctggcagctg
     2821 cgggatgcac ttttgacgat atcattgatg ttacgagctt tcataccgat ccagaaaaac
     2881 aatttgaaga catcatgacg gtgaaaaatg aaatatttag cgcgccacct tatccaacct
     2941 ggacggcggt gggcgttaca tggctggcag gctttgattt tgaaattaaa gtgatagcgc
     3001 gcatccctga gctgtaagca atagtgttag ccgttcgttt tcacactccg ccctataagt
     3061 cggatgaatg gaataaaatg catatctgat tgcgtgaaag tgaaaaagaa aaaagcagga
     3121 aatgtctgca attattgata ccgaacgaca gttccccctc cagaatcaca gtaaataaaa
     3181 atgcatatat tttgacttta aattcaaata aaccgtttgc gctgacaaaa tattgcatca
     3241 aatgcttgca ccgcttctga cgatgagtat aatgccggac aatttgccgg gaggatgtat
     3301 ggttcagtgt gttcgacatt ttgtcttacc gcgtctgaaa aaagacgctg gcctgccgtt
     3361 tttcttcccg ttgatcaccc attcccagcc cctcaatcga ggggcttttt tttgcccagg
     3421 cgtcaggaga taaaagatgg ctaatccgct atatcagaaa catatcattt ccataaacga
     3481 ccttagtcgc gatgacctta atctggtgct ggcaacagcg gcgaaactga aagcaaaccc
     3541 gcaaccagag ctgttgaagc acaaagtcat tgccagctgt ttcttcgaag cctctacccg
     3601 tacccgcctc tctttcgaaa cttccatgca ccgcctgggt gccagcgtgg tgggcttctc
     3661 cgacagcgcc aatacatcac tgggtaaaaa gggcgaaacg ctggccgata ccatttcggt
     3721 tatcagcact tacgtcgatg cgatagtgat gcgtcatccg caggaaggtg cggcgcgcct
     3781 ggccaccgag ttttccggca atgtaccggt actgaatgcc ggtgatggct ccaaccaaca
     3841 tccgacgcaa accttgctgg acttattcac tattcaggaa acccaggggc gtctggacaa
     3901 tctccacgtc gcaatggttg gtgacctgaa atatggccgc accgttcact ccctgactca
     3961 ggcgttagcg aagttcgacg gcaaccgttt ttacttcatc gcgccggacg cgctggcaat
     4021 gccgcaatac attctggata tgctcgatga aaaagggatc gcatggagtc tgcacagctc
     4081 tattgaagaa gtgatggcgg aagttgacat cctgtacatg acccgcgtgc aaaaagagcg
     4141 tctggacccg tccgagtacg ccaacgtgaa agcgcagttt gttcttcgcg ccagcgatct
     4201 ccacaacgcc aaagccaata tgaaagtact gcatccgctg ccgcgtgttg atgagattgc
     4261 gacggatgtt gataaaacgc cacacgcctg gtacttccag caggcaggca acgggatttt
     4321 cgctcgccag gcgttactgg cactggttct gaatcgcgat ctggtactgt aaggggaaat
     4381 agagatgaca cacgataata aattgcaggt tgaagctatt aaacgcggca cggtaattga
     4441 ccatatcccc gcccagatcg gttttaagct gttgagtctg ttcaagctga ccgaaacgga
     4501 tcagcgcatc accatcggtc tgaacttgcc ttctggcgag atgggccgca aagatctgat
     4561 caaaatcgaa aatacctttt tgagtgaaga ccaggtagat caactggcat tgtatgcgcc
     4621 gcaagccacg gttaaccgta tcgacaacta tgaagtggtg ggtaaatcgc gcccaagtct
     4681 gccggagcgc atcgacaatg tgctggtctg cccgaacagc aactgtatca gccatgccga
     4741 accggtttct tccagctttg ccgtgcgaaa acgcgccaat gacatcgcgc tcaaatgcaa
     4801 atactgtgaa aaagagtttt cccataatgt ggtgctggcc aattaattgc ggttggtaat
     4861 aaaagtctgg ctccctataa tgagccagac tttttaccgc tgtaataaag gagaaatcat
     4921 gagcaaaact atcgcgacgg aaaatgcacc ggcagctatc ggcccttacg tacagggcgt
     4981 tgatctgggc aatatgatca tcacctccgg tcagatcccg gtaaatccga aaacgggcga
     5041 agtaccggca gacgtcgctg cacaggcacg tcagtcgctg gataacgtaa aagcgatcgt
     5101 cgaagccgct ggtctgaaag tgggcgacat cgttaaaact accgtgtttg taaaagatct
     5161 gaacgacttc gcaaccgtaa acgccactta cgaagccttc ttcaccgaac acaacgccac
     5221 cttcccggca cgttcttgcg ttgaagttgc ccgtctgccg aaagacgtga agattgagat
     5281 cgaagcgatc gctgttcgtc gctaatcttg atggaaatcc gggctatcat gcccggatta
     5341 agtctgatga caaacgcaaa atcgcctgat gcgctacgct tatcaggcct acgtgattcc
     5401 tgcaatttat tgaatttgtt ggccggataa ggcatttacg ccgcatccgg catgaacaaa
     5461 actcactttg tctgcaatct gaatccgggt caccatgccc agttgattct ttattgccag
     5521 ccgtaacgac ggctatagaa ccctttcacc aactgggtta atgtcatata ccctgccaga
     5581 atcgcaacca gccacgggaa atagcttaac ggcagcgcct gcagctgcag atagctggcc
     5641 agcggtgaaa acggcaatgc gataccgaca atcatcacga tcacggtcat gatcattaac
     5701 ggccacgatg cacagctctg aataaacggc acacggcggg tgcggatcat atgcacaatc
     5761 agcgtttgcg acagtaagcc caccacaaac catcccgact ggaagagcgt ttgcgtttcc
     5821 ggcgtgttgg catggaatac ccaccacatc aggcaaaacg tcagaatatc gaagatcgag
     5881 ctgatcggtc cgaagaagat cataaagcgc cccagatccg ccggattcca acgctgcggc
     5941 ttttgaattt gctcgtcgtc gacgttatca aacgggatcg ccacctgtga cacatcgtac
     6001 agcaggttct gaataagcaa gtgtaacggc aacatcggca ggaagggcaa gaaagcactc
     6061 gctaccagca cgctgaacac attaccgaag ttagagctcg ccgtcatttt gatgtatttc
     6121 agcatgttgg cgaaagtgcg tcgtccctca ataaccccct cttccagcac catcaggctt
     6181 ttttccagca ggatgatatc agccgcttca cgggcaatat ctaccgcgcc gtccacagaa
     6241 atgccgatat ccgcagcgcg taaggcgggc gcatcattaa taccatcgcc cataaagcca
     6301 accacatgcc cttcgcgctt cagtaaggtc acaatgcgtt ctttatgcat cggcgtcagg
     6361 cgggcaaaca gcgtggtgcg ctgtgcaaga tttgccagtt cgtcgtcaga tagcgtttca
     6421 atatcactac caatgaccac ctctcccgcg tccagcccca cttcatggca cactttcgtt
     6481 gcaactaact cactgtcgcc agtaaggatt tttacggtaa ttccactcgc ttttaatgcc
     6541 ttcagtgccg gagcggttgt ctcttttggc ggatcaagaa aagcaatata tccttcgagg
     6601 atcaggtcgg attcatccgc ccgctggtaa tctccttcac gcgctggcag gtatttcgtc
     6661 gccaccgcaa ccacgcgcag cccctgacga ttcagcgtat cagtaacccg cttaatctta
     6721 cgcagcatga tgtcatcgag cggcacaatc tcgccattgt gacgcacctg cgaacacaca
     6781 ttgaggattt cctgcaatgc acctttgcaa accagctgat ggtgctcggt attttctgcc
     6841 actaccaccg acatccggcg acgctcgaaa tcaaacggaa tctcatcgat tttttgccaa
     6901 cgactggcca gcgagcgcgc tgactcttca tccgtacctt cgagcaccgc tgtatcgagc
     6961 aggtttttaa gtccggtctg ataatgactg ttcaaccacg cgctatgcag cacgcgttcg
     7021 ctggttttac cggagatatc ggtatgattc tccagcacaa ttttatcctg tgtcagggtg
     7081 ccggttttat cagtgcacag aatatccatt gcgccaaagt tctgaatagc atccagatgt
     7141 ttgacgatca ctttctgttt cgacagcttt actgcgccgc gcgccagcgt cgaggtaaca
     7201 atcatcggca acatttccgg cgttaggcct accgcgaccg aaagcgcaaa cagcgccgct
     7261 tcccaccagt cgcctttggt gtagccattg attaacagca ccaccggtgc catcaccagc
     7321 ataaagcgaa tcagcagcat actgacgcgg ctgatccctt gctgaaaggc attcggctcg
     7381 ctttcctgct cactaacacg ccccgccagt tgaccaaacc aggtattggc acctgtagca
     7441 atcaccattg cttgtgccgt gccgctcacc acggtggtgc ccataaaaca cagcgtgtcg
     7501 cactccagcg gattgctgtg ctccggctgg cgagtggtag cggctttttc cacgggcaga
     7561 gactcaccgg ttaacgacgc ctgagcgacg aacagatccc gcgcctgcaa gatacgtaaa
     7621 tctgccggga tcatatctcc cgccgccagt tttataatat cgccgggcac cagctggtcg
     7681 atcgggatct ccagccagcc attttcgcct ttgtcgttaa ttacgcgcag caccgtcgcg
     7741 gtattgctga ccatcgcttt cagggcatct gccgctttag tggaacgtgc ttcctgaata
     7801 aagttcagca acgttgaaat agcgaccatt agcgcgataa cgcccgcggc aaataaatct
     7861 tccgtggcgt aagaaatagc gccgagaatg gtgagtaaaa tattaaaggg gttgcgatag
     7921 cagacccata aatgtaccca ccacggcgac ggttgttgtg cgggtaattt attttcacca
     7981 tgttgttcgc gggcagattc cacttccgcc tgatgtaacc cttccggatg ggtgtcgaac
     8041 gttttccaca attcttcttc gggcatcacc gccattttca ggcaatgcgc acttaaggac
     8101 ggcgggacca ccgtattcac tgtctgctgc acgccaggca atggatcacg atgaaccaga
     8161 cgggaaggta aatggcgagt gagccgggta aaaatttctt taaacataag gagtccctcc
     8221 gcactgtctg aaacagtaca gaaatgtctt caggcgtggc gaagccttac ctgaaggtaa
     8281 taaagctgag cagggacgtt aaaacgtcac ggccttacgc atccggtaac gccgtgaaaa
     8341 gacgggctta ccggaaaagt tttaatctcc gtcgagggag aggcgtggga tcaggttcca
     8401 tataacctcc ggtaagtgaa aaaagcgccg caaataataa ggagaatcca ataatattcg
     8461 tagcatttat ggcgatataa tacctgcagg caactaaacc aacgataaac cagactttac
     8521 ccattgctga atgcacgggt aaagttagcc tcaaataatt aaacaacacg ttacgggaca
     8581 acaggatgca aaatcggctg accatcaaag acatcgcgcg cttaagcggc gtggggaaat
     8641 ctacggtttc ccgggtgctg aataacgaaa gcggcgtgag ccagcgcacg cgcgagcgtg
     8701 ttgaagcagt gatgaatcag catggatttt ccccttcccg ttctgcacgc gctatgcgtg
     8761 ggcaaagcga taaagtggtc gccatcattg tcacccgtct ggattcgttg tcagaaaatc
     8821 tcgccgttca aaccatgctg ccagcgttct atgaacaagg ttacgatcca atcatgatgg
     8881 aaagtcagtt ttccccgcaa ttagttgccg aacatttggg ggtgctgaaa cggcgtaata
     8941 tcgacggcgt agtgctgttc ggttttaccg gcataacaga agaaatgtta gcccactggc
     9001 agtcatcgct ggttctgctg gcgcgtgacg caaaaggctt tgcttcggtc tgttatgacg
     9061 acgaaggggc aatcaagatc ctgatgcaac ggctgtatga ccaggggcat cgcaatatca
     9121 gttatctcgg cgtgccgcat agtgacgtga cgaccgggaa gcgacgtcac gaagcctacc
     9181 tggcgttctg caaagcgcat aaactgcatc ccgttgccgc cctgccaggc cttgctatga
     9241 agcaaggcta tgagaacgta gcaaaagtga ttacgcctga aactaccgcc ttactgtgcg
     9301 caaccgacac gctggcactt ggcgcaagta aatacctgca agagcaacgc atcgacacct
     9361 tgcaactggc gagcgtcggt aatacgccat taatgaaatt ccttcatccg gagatcgtca
     9421 ccgtagatcc tggttacgcc gaagctggac gccaggcggc ctgccagttg atcgcgcagg
     9481 taaccggacg cagcgaaccg caacaaatca tcatccccgc caccctgtcc tgatcgtttc
     9541 ctgaacgata aattgtgatc ttcgctgcgt ttcgggaacg ttcccgtttt taaatttttc
     9601 cgcgcaatat attctgcagc caaccaaaaa tgtcatctgc catggggctt tatgatgagc
     9661 aaaataaacc aaacggatat cgatcggttg attgaactgg tcggcgggcg cggcaatatt
     9721 gcgacggtga gccactgtat tactcgcctg cgctttgtcc tcaaccaacc ggccaatgcc
     9781 agaccgaaag aaattgagca actccccatg gtgaaaggct gtttcaccaa tgccgggcaa
     9841 tttcaggtgg tgattggcac caacgtgggt gattactatc aagcactgat agcgtcaacc
     9901 ggacaggcgc aggttgataa agagcaggta aaaaaagccg cccggcagaa tatgaaatgg
     9961 catgagcagt tgatctctca tttcgcggag atcttcttcc cgttgctgcc cgcgttgatt
    10021 agcggcggtt tgatcctcgg ttttcgcaat gtgatcggcg atttgcccat gagcaacggt
    10081 caaacgctgg cgcaaatgta cccttccctg caaacgatct acgattttct gtggttgatc
    10141 ggtgaagcga tcttcttcta cctgccggtc gggatttgct ggtcggcggt gaaaaaaatg
    10201 ggcggcacgc cgatccttgg tatcgtactt ggcgtgacac tggtttcccc ccagctgatg
    10261 aacgcttatc tgctcgggca gcagctgccg gaagtgtggg actttggcat gttcagcatc
    10321 gccaaagtgg gctatcaggc gcaggtgatc ccggcactgt tagccgggct ggcgctgggc
    10381 gttattgaaa ctcgccttaa acgcatcgtg ccagattacc tctatctggt ggtggtgccc
    10441 gtctgttcgc tgatcctcgc ggtgttcctc gcccatgcgc tgattggtcc gtttggtcgc
    10501 atgattggcg atggcgttgc ctttgcggta cgtcacctga tgaccggcag ctttgctccg
    10561 attggcgcgg cattgtttgg cttcctgtac gccccgctgg tgatcaccgg ggtacaccag
    10621 accacgcttg ctattgattt gcagatgatt caaagcatgg gtggtacgcc agtgtggccg
    10681 ctgattgcgc tgtcgaatat cgctcagggc tccgccgtga taggcattat catttccagc
    10741 cgcaagcaca atgaacgcga gatctccgtt cctgccgcta tctccgcctg gcttggggtc
    10801 actgagcctg caatgtacgg catcaacctg aaatatcgct tcccgatgct gtgcgcgatg
    10861 attggttctg gtctggcagg attgctatgt ggcctgaacg gcgttatggc gaatggcatc
    10921 ggcgtaggcg gcctgccggg aattctctcg attcaaccga gctactggca ggtgtttgcg
    10981 ctggcaatgg ctatcgccat catcatcccg attgtactca cctcgtttat ctatcagcgg
    11041 aaataccgcc tgggcacgct ggacattgtt taattttctt cggggcgcaa ttgcgccccc
    11101 tcgcatttgc aggaataacg taatgactaa tcttccccac tggtggcaaa acggcgttat
    11161 ctaccagatt tatccaaaga gttttcagga caccacgggt agcggcaccg gcgatttacg
    11221 tggcgttatc caacgcctgg actatctgca taaactgggc gttgatgcca tctggctaac
    11281 ccccttttat gtctctccgc aggtcgataa cggttacgat gtagcgaact atacggcgat
    11341 tgatcccacc tacggcacgc tggacgattt tgacgaactg gtgacgcagg caaaatcgcg
    11401 tgggattcgt atcattctcg atatggtgtt taaccatacc tctacccaac atgcctggtt
    11461 tcgcgaggcg ctgaacaaag aaagccctta ccgccagttt tatatctggc gcgatggcga
    11521 accagaaacg ccaccgaaca actggcgttc aaaatttggc ggtagtgcgt ggcgctggca
    11581 tgcggaaagc gaacagtact atttgcatct ctttgcacca gaacaggcgg atctcaactg
    11641 ggagaatcca gcggtacgcg cagagctgaa aaaagtctgt gagttctggg ccgatcgtgg
    11701 ggtcgacggg ttgcgcctgg atgtggtgaa tctgatctcc aaagacccgc gtttccctga
    11761 agacctggac ggcgacgggc gtcgcttcta caccgacggg ccacgagcac acgagttttt
    11821 gcacgagatg aaccgcgatg tgtttacgcc acgcgggtta atgaccgtag gtgaaatgtc
    11881 ctccaccagc cttgagcatt gccagcgata cgcggcactg acaggcagtg aattatcgat
    11941 gacctttaat tttcatcacc tgaaggtcga ttatcccggt ggtgaaaaat ggacgctggc
    12001 taaacctgac tttgtggcgt tgaaaacatt gttccgccac tggcaacaag gaatgcacaa
    12061 cgtagcatgg aatgccttgt tctggtgtaa ccacgatcag ccgcgcattg tttctcgctt
    12121 tggtgatgaa ggtgaatacc gcgtgcctgc ggcaaaaatg ctggcgatgg tgctgcatgg
    12181 catgcaggga acgccgtata tctaccaggg cgaagagatt ggcatgacca acccacattt
    12241 cacgcgcatt actgactatc gcgacgtgga gagcctcaat atgtttgccg agctgcgcaa
    12301 cgatgggcgt gatgccgacg agttattggc aatcctcgcc agtaaatccc gtgacaacag
    12361 tcgcacgccc atgcaatgga gcaacggcga taatgccggg tttacggctg gcgaaccgtg
    12421 gattggcctg ggcgataact atcaacaaat caacgtagaa gccgcgctgg ccgatgattc
    12481 ctcggtgttt tacacctacc aaaagttaat cgcactgcgt aagcaagaag ccatcctgac
    12541 atggggcaat taccaggatc tgctgccaaa cagccctgta ttgtggtgct atcgccgtga
    12601 atggaagggg caaaccttgc tggtcattgc caaccttagc cgtgagatcc aaccctggca
    12661 gccagggaaa atgcgcggca actggcaact tgtgatgcat aactacgaag aagcctcacc
    12721 acaaccctgt gccatgactt tacggccttt tgaggctgtc tggtggttac agaagtaaat
    12781 cttccctaag ccccggtaat gccggtcatt ccggggtttt gtctgtcagt atgttgtttt
    12841 tgttgatttt tcaaccagaa aattcattaa aaaatttaca tatcgctgta gcgcccgtca
    12901 tccgtacgct ctgcttttta ctttgagcta catcaaaaaa agttaaaaca tccttgatgc
    12961 aaagcactat atatagactt taaaatgcgt cccaacccaa tatgttgtat taatcgacta
    13021 taattgctac tacagctccc cacgaaaaag gtgcggcgtt gtggataagc ggatggcgat
    13081 tgcggaaagc accggaaaac gaaacgaaaa aaccggaaaa cgcctttccc aatttctgtg
    13141 gataacctgt tcttaaaaat atggagcgat catgacaccg catgtgatga aacgagacgg
    13201 ctgcaaagtg ccgtttaaat cagagcgcat caaagaagcg attctgcgtg cagctaaagc
    13261 agcggaagtc gatgatgccg attattgcgc cactgttgcc gcggttgtca gcgagcagat
    13321 gcagggccgc aaccaggtgg atatcaacga gatccagacc gcagttgaaa accagctgat
    13381 gtcgggtcca tacaaacaac tggctcgtgc ttacatcgag taccgtcacg atcgcgacat
    13441 tgaacgtgaa aaacgcggtc gcctgaacca ggagatccgt ggtctggtcg agcagaccaa
    13501 cgcctcgtta ctcaacgaaa acgccaacaa agacagcaag gtgattccaa cccagcgcga
    13561 cctgctggcc gggatcgtgg ctaaacacta tgcacgccag cacctgctgc cgcgtgacgt
    13621 ggtgcaggca catgagcgtg gcgatattca ctatcacgat ctcgattact cgccgttctt
    13681 cccgatgttc aactgcatgt tgatcgacct gaaaggcatg ctgacccagg gctttaaaat
    13741 ggggaacgcc gagattgaac cgccgaagtc gatctctacg gcaaccgcgg taactgcgca
    13801 gattattgct caggtcgcca gccatattta tggcggcacc accattaacc gtatcgatga
    13861 agtgctggcg ccgtttgtta ccgccagcta caacaaacat cgcaagaccg cagaagagtg
    13921 gaacatcccg gacgccgaag gctacgctaa ctctcgtacc atcaaagagt gctacgatgc
    13981 cttccagtcg ctggagtacg aagtaaacac cctgcacacc gccaacggtc agacgccgtt
    14041 tgtaaccttt ggttttggtc tgggcaccag ctgggaatcg cgcctgattc aggaatcgat
    14101 cctgcgtaac cgtatcgcag gcctcggtaa aaaccgtaaa actgcggtgt tcccgaaact
    14161 ggtgtttgcg attcgcgatg gcctgaacca taaaaaaggc gatccgaact acgacatcaa
    14221 acagctggcg ctggagtgcg caagcaagcg catgtatccg gatatcctga actacgatca
    14281 ggtagtgaaa gtcaccggtt cgtttaaaac cccgatgggc tgccgcagct tcctcggcgt
    14341 gtgggaaaat gaaaacggcg agcagatcca cgatggtcgt aacaacctcg gcgtgatcag
    14401 cctgaacctg ccgcgtattg ctctggaagc aaaaggcgat gaagccacct tctggaagct
    14461 gctggatgaa cgtctggtgc tggcacgtaa ggcactgatg acccgtatcg ctcgtctcga
    14521 aggcgtgaaa gcgcgcgtcg ccccgatcct ctatatggaa ggtgcttgtg gcgtacgtct
    14581 gaatgctgac gatgatgttt ctgaaatctt caaaaacggt cgtgcgtcta tttcgctggg
    14641 ttacatcggc atccacgaaa ccattaacgc gctgttcggc ggcgagcatg tttacgacaa
    14701 cgagcagctt cgcgcgaaag gtatcgccat tgttgaacgt ctgcgtcagg cagtggatca
    14761 gtggaaagaa gaaacgggtt atggtttcag tctctacagc acgccgagtg aaaacctgtg
    14821 cgaccgcttc tgccgtctcg atactgctga gtttggcgtg gtgccgggcg tgaccgataa
    14881 aggttactac accaacagtt tccaccttga tgtggagaag aaggtgaacc cgtacgacaa
    14941 gatcgacttt gaagcgcctt acccgccgct ggcgaacggt ggtttcattt gctacggcga
    15001 gtatccaaac attcagcaca acctgaaagc gctggaagat gtctgggatt acagctatca
    15061 gcatgtaccg tattacggca ccaatacacc gattgatgag tgctacgagt gtggctttac
    15121 cggtgagttc gagtgcacca gcaaaggctt cacttgcccg aaatgtggta accatgacgc
    15181 ctcccgtgtg tcggtaactc gccgcgtgtg cggatattta ggtagcccgg atgcacgtcc
    15241 gtttaacgct ggtaagcagg aagaagttaa gcgccgcgtt aaacatttgg ggaatgggca
    15301 gataggttaa tcttcactct gcgctaagca aatgccggat gcggcgtaaa cgccttatcc
    15361 ggcctacatt tggcaactgc tccaggcctg ataagacgcg acagcgtcgc atcaggcatt
    15421 gattaccgga tgcagcctac acgcggactg ataagccaag cgaaataatg aattatcatc
    15481 agtactatcc tgtcgacatc gtcaacggcc ccggcactcg ctgcaccctg tttgtctccg
    15541 ggtgtgttca tgaatgcccc ggttgctata acaaaagcac atggcgggta aattctggtc
    15601 agccatttac caaagcaatg gaagacaaga tcattaaaga tctgaatgac actcgtatca
    15661 aacgccaggg gatttccctc tccggcggcg atccgctgca tccgcaaaac gtgccggata
    15721 ttctgaaact ggtaaaacgc atccgcgccg agtgtccggg taaagacatc tgggtgtgga
    15781 caggctataa actcgacgaa ctcaacgctg cgcaaatgca ggttgttgat ttgattaacg
    15841 tgctggtcga cggcaaattt gtgcaggatt taaaagaccc ttccctgatc tggcgcggca
    15901 gcagcaatca ggtggtgcat catttgcgat gataagttcc ctgttacctg agtgcaggca
    15961 acagggaaat gaggaattaa cgatacttct ggtgataggc gttgcgggtc gttttcagtt
    16021 gctctgcagc agcctgcgct tcttttactt taccttcatt tgccagcttc agcgcgtcgt
    16081 caatctgacc gaccagaatg tcgaaaccgt ggcggaaatc tttcatttcc gggctgtccg
    16141 gtgatttatc ttcgagcttc ggcggcgttg ctttttgcgc atccagcgct gcggcgcgca
    16201 tcttcgttaa cgcgtctttg acttgcgccg cgttatccgc tttttcgatc acttttaaat
    16261 tgtcgttgag ggtttccata ttgtcttcaa gatcagcggc aaacgacgca gaactgaata
    16321 ccaacgagga gactgcaaga atagctaaca ggcttttacg cattgctcac ttcctttttt
    16381 attatgaaga tttggcgcgg caggtcgcgc cagttttttt actgcccggc gattttcatc
    16441 tccggcaaca gcactgaacc acactgtata ttactgcgtg tttcaatatc gttaccgacg
    16501 gtgacaatat tgcgccacat atcttttaaa ttacctgcga tggtgatttc actaacggga
    16561 tactgaattt cgccattctc tacccagaag cccgcagcac cacgggaata atcaccggta
    16621 atggcactca cgccctggcc catcagctcc gtcaccacca gcccggtgcc catctctttg
    16681 agcatttgtt cgaagcttag cccttgtccg gcaatccgcc agttgtgaat accgcccgca
    16741 tgtccggtgc ttttcagccc cagttttcgc gccgaatagc tggtcagcag ccactgagtc
    16801 aggatgccat ctttaataat atcgcgacgc tcggtgcgca caccttcgct gtcgaatggc
    16861 gtcgacgcca gccctttcag cagatgcgga tgctcttcaa tggtcagcca gtccggcaga
    16921 atttgtttac ccagcgaatc cagcaggaag gtagatttac gataaaccga tccacccgct
    16981 atcgccccca ccagatgccc aaaaagccca gttgccactt cattggcaaa aatgactggc
    17041 gctttcatgg tggagagttt acgcggtgac aggcgcgata aagtacggcg agcacagtcg
    17101 gccccaaccc actctggcgt ttgcagatcg ctcatcgcgc gcccaatggt gtaggcgtaa
    17161 tcacgctcca tatcgccatt ttcttcggca attacacagc tggagagcga atgacgcgtt
    17221 gagcagtaac cctgcaacat gccgtggctg ttgccaaaaa ctttgacacc gtagtggctg
    17281 ttaaagctgc caccttcggt attggtgata cgtttatccg cctgtaatgc tgcctgttct
    17341 gcgcgggccg ccagttcaat ggcttcatcc ggggaaactt ccgcagggtg gaacaagtcg
    17401 agatctggtg cgtcaaaggc cagcagctct ttgtctgcca ggccggcaca gggatctggc
    17461 gaggtataac gggcaatatc cagcgccgcc tgtacagtac gggcaatggc ctgcgggctt
    17521 aaatcggtgg atgatgcgct acctttgcgg ttctggtgat aaacagtgat ccccagcgcg
    17581 ccatcgctat tgaattcaac attctccact tcaccataac gcgtgcttac gctaatgccg
    17641 gtggtcttgc tgacggcaac ttccgcaccg tccgatttgc ctgaggccaa ctccagcgca
    17701 gtggaaactg cttcttccag aatcttgcgc tgcgcttcaa cttgagagat tactttcatt
    17761 gcaagtgcca taatgtagag agtttctctg aagtctaaca gagaaccgtt tttcagtgcg
    17821 catcttaact ggtaacatta gcctcttttt ttaaggagcc tgagatgact aagcagcccg
    17881 aagactggct cgacgacgtt cccggtgatg acatcgaaga cgaagacgat gaaattatct
    17941 gggtcagtaa aagtgaaatt aaacgtgatg ccgaggagct aaaacgcctt ggcgcggaaa
    18001 ttgttgatct ggggaaaaac gcgctggata agatcccgtt agatgcggat ctgcgcgcgg
    18061 ctattgagct cgcccagcgt attaagatgg aaggtcgccg ccgccagttg cagcttatcg
    18121 gtaagatgct gcgccagcgc gacgtagagc ctattcgtca ggcgctggat aagctaaaaa
    18181 accgtcacaa ccagcaggtg gtgctgttcc ataaactgga aaatctgcgc gatcgtctga
    18241 tcgaccaggg tgatgacgcc atcgctgaag tattgaatct gtggcccgat gccgatcgtc
    18301 agcaactgcg tactctgatc cgcaacgcga agaaagagaa agaagggaat aagccgccga
    18361 aatccgcacg ccagattttc cagtatctgc gcaaactggc ggagaatgaa gggtaatttt
    18421 cgttgttgat gtgagtatgg tttgccggat gcggtgcacc tgtaggcctg ataagacgca
    18481 tcaagcgtcg catcaggcac tatgctcaaa tgccggatgc ggcgtgaacg ccttatccgg
    18541 cctacgcaca ctatatcagg ctgaggccga attactgcgc agcttccgcc ttcttcgcca
    18601 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    18661 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    18721 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18781 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18841 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18901 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18961 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    19021 taaacgaacc cagcgcgtta gcggcatcta ccggcgctac accaacatgg cgagccgctg
    19081 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    19141 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    19201 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    19261 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    19321 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    19381 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    19441 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    19501 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    19561 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    19621 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    19681 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    19741 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19801 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19861 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19921 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19981 acgcatatgt cgggcaagtg agaaatgcat ttcaggataa tccgcaattt gctggcgcga
    20041 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    20101 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    20161 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    20221 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    20281 ctgagaacgt gcagggcgag gttcagcaga aactcgactt gttcgctaat gaaaaactga
    20341 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    20401 ttgtcgtctt tgaaggctgt gaacacgcaa aatacgtggt gctgatggac cccctggatg
    20461 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    20521 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    20581 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    20641 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    20701 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    20761 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaaccgcc
    20821 cgtatacctc acgttatatc ggttcactgg tcgcggattt ccaccgtaac ctgctgaaag
    20881 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20941 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    21001 aagagcgtat tctggatatc atcccggaaa ctctgcacca gcgccgttcg ttctttgtcg
    21061 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    21121 ctcctgcgcc tctccacacg gagaggcgtt tttatcgctg cgcaatgttg actcttgtca
    21181 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    21241 taaacaacaa aataccgatg gcgattttcg tccaccatga gctcagcgtg ccatcaaagt
    21301 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    21361 caacgccacc gctcagaagc gtaccgccaa ttaccactga ggcgatagca tccagttcca
    21421 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    21481 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    21541 ccatcaggtt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    21601 tacgatgcgc gaggaaaata ccgataacca ccaccgccaa catcagcagt cccatcgcac
    21661 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    21721 ggtttatcgg aatcgactct tccgaaacga gatagctgac accgcgcaaa aagaacatcc
    21781 ccgccagggt aatgataaat gccgggatct tcagggcgtc gatcaaaagc cccataaatg
    21841 cgccgaaggc acagcccatc accagcacta gcggaaacgc cagtagcggc gagaggccga
    21901 aatcgccaat cacttttgcc agaaacacgc cagtaaaggc gatcaccgaa ccgacggaga
    21961 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    22021 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccggga aactgggtca
    22081 ggcagtaaag ataacccaac acaaagacgc cgatggtgat catcagtggc aaattacgtt
    22141 ttatcacggc tacgtactcc tttaatcaga ctgataaagc gttgcgactg gacaatcagc
    22201 acgcaaagaa ccaccaccgc ttttacaacc tggttcatct ctggcggaaa gcccgaaagc
    22261 aaaattccgg tgttcatccc ctgaataatc agcgccccca ccaccgaaag cagtaggtta
    22321 aaacgtccgc ccatcagcga tccgccgcca ataaccaccg cgagaatggc gtccagctcc
    22381 agccataacc cggcgttatt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    22441 gcaatcgccg cacacagccc gctcaacacg taggtaagca tgacgatgat ccgcgtgttt
    22501 accccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    22561 gccgttttgc gggtcaacag ccagaacagg ataagcgtca gcaccgcgat aatgaccggc
    22621 gttggcagga acaacagcga tccactgcca aaccatgaga gatccggcga gttaaacgtg
    22681 acgatctgtc cggaggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    22741 gtggcaacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgcc
    22801 aggatgccag tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22861 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacggagag atcaatccca
    22921 cctgttgcga tcaccagcgt cattccgata gccaacagcg caacgggggc cgcacggtta
    22981 agaatgtcta tggggctacc gaataaacgc ccatcctgga gcaccacctg ccagaaatgc
    23041 ggggccacca ggctatcgac cagcagcacc agcaacagtt ccgccagctg cggcattccc
    23101 gttggccagc gaaagcgcct tttcggcggc gtagtgtccg ggagagattg aggcatcaca
    23161 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct ctgccagcgg
    23221 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    23281 ttcttccagt tcggaggaga tcaccagcag cgccagacca tcggcgcata gcgtttcaat
    23341 caggcggatg atctcggcgt gggcaccaac atcaatgccg cgagttggct catcaaggat
    23401 cagaaattgc ggtcgggtca gtagccaacg tgaaagcaac actttttgct gattgccgcc
    23461 agagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    23521 gcgttcggca atctcttgct gttctttgcg ggaaatggga cgtagccagc cgcgctgggc
    23581 ctggagagcg aggatgatat tttcccgcac cgaggcggca gcgatgatgc catcggtttt
    23641 cctgtcttcc gggcagaagc caatgcccag taccgaagcc tgatgtggcg atcgcaggtt
    23701 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    23761 ttcggcggtt tcggtacgtc ctgatcccag caatccagcc agaccgacga tctcgccggg
    23821 gcgtacttcg agatcaaacg gtgcgatcgt tccttttttg ccgtaatttt tgaacgcggc
    23881 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcgcgtggg tatccagctc
    23941 gcgcccccgc atcattttta ccagttcgat ctgcggtagc tcgcacgttt cccgacagcc
    24001 tacgaaactg ccgttgcgta agacggtgat ccgatcgctg acctgataga cctgatcgag
    24061 aaagtgagtg acaaaaatca ggctgacgcc gcgatcgcgc aactgacgca tcaggtcaaa
    24121 cagtaactcc acttcctggg tgtcgagact ggcggtgggt tcatcgagga tcagcacttt
    24181 ggcagagaga tcgatagccc ggcaaatagc gacgatttgc tgcatcgcga ctgaaaagcg
    24241 gttgagcggt tcgcgcacgt cgagggagaa accgtaagat gccatcagtt cggtggcgcg
    24301 cttttccatc tctttgcggc gtagaaggcc gaagcgtttg ggttcgcggc ctataaatag
    24361 attatcagcg accgacatat tgggtagcag gtttacttcc tgatagacgg tgccgatacc
    24421 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    24481 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcctgcccc
    24541 gttttcaccg agcagcgcca tgatttcgcc gcggcgcagg ctgaaatcaa cgttgtctaa
    24601 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    24661 gttagtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    24721 gtaagcgtcg catcaggctt cagcgcacca ccgccggatg cggcgcgagc gccttatccg
    24781 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24841 actcttcttt tgcggtatca ggcagataaa gggtggattt ggtcagcgtc agcttttcag
    24901 gcatggtgcc gtcttttttg tatttctcca gcgcgtcgaa ggcgggacct gccatattcg
    24961 gcgtcagttc aacactggcg ttcgcttcgc catcaatcat cgctttgtag atatccggta
    25021 cgccgtcgat agaacctgtc agaatatctt tgcctggttt caggcccgct tctttaattg
    25081 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    25141 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    25201 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    25261 ctttcttacg gtcaatggcg acgctggccc caacagtgcc ctgcagctcc accacgttgc
    25321 atggtttgcc attcacttct tttaccagcc agtcaccaat caacttgcct tcgagaatgt
    25381 tgtcggcagt gacggtggtc atatagagag atttgtcttt cacatcaata gaacgatcga
    25441 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caaccagtcg
    25501 caaccaccgg agcgataaag atcgcatcca ccccttgcgc gacgaaggaa cgtaccgctt
    25561 taatctggtt ttcctgcttt tgctgaccat cggcaatttt cagcgtaatt ccgcgttttt
    25621 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccagattcc gatccgacct
    25681 gcgaaaatcc tacggttaat ggagcggcca acgccataga cgacatggct gccgagactg
    25741 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25801 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25861 acacttcaaa gaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25921 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaattggcga
    25981 aaaaggaaaa tgaagacgaa aacaagcgaa gacattcggc gcgagttggc tataatactc
    26041 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    26101 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    26161 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    26221 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    26281 cccggttgac gtactggtcc cgactccgta cccgctgcag ccgggttctg tgatccgttg
    26341 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactgattgc
    26401 tgttccgcac accaagctga gcaaagaata cgatcacatt aaagacgtta acgatctgcc
    26461 agagctgctg aaagcgcaga tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    26521 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    26581 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    26641 cttcggttag taagggtttt tttatgcccg cgataaataa actctctatt ccaccatcat
    26701 tattctcagc ggttgcaagg cttgaacggt aagaacaagc aaacccgacc accattttgc
    26761 tgttcatagc cacttgctgg aagttagccg acctcactca tactcaccgt tcaaagcgtg
    26821 cccccgtttt acccctgcac atttgtgaac gccagcagag ttttttatgc gattgcagat
    26881 atggaaagcg gctcggagag aaaagcaaaa gatggaaaag ttacaaaaaa aaatcgcgag
    26941 atacatcgca taaaacaacg ccacccgaag gtggcgtgct tttattatta cttatccctg
    27001 tctaaccagt cgccgctttc aattagcttt aatccatcga cgggtcgttg ataaacgtac
    27061 atccatgcac tcccgtacgg cgtctgaatc aactggcgcg cgtattcacc gcccctggtg
    27121 cgcaaggcat caagttcggc cagcgtggcg ttgtcaatac gataaacttc accgtgtact
    27181 gttccgttcc ccggaactgc gcctggatag tggcccaggc tatacaactg gtagttatcg
    27241 atactgaaat cgcccagtaa ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg
    27301 cgtaaactgc cgtagacaaa tattcgcatt gctaaaactc gaactgatag agcaaatcca
    27361 gtgcctggtc tacaccagac acggcttcca gatatagctt aggcatcagg cgataacgta
    27421 acgtgagtgt tgctatagag tcaaatatac ccacgccgta tttcacttgc agacctggca
    27481 atacatagcc gctgaccact acctgggagg agtcgcctac tccctgggtg tcgagcgcta
    27541 aattgcttac gccaaacgtc tcgccgattt tacccacaat ctggccactt tgcgcaaccc
    27601 ccagaccaat cagcatcgag gtcattgccg cactgtcgct ctgatcgctc tccagccctt
    27661 gtccacgtag caaataagac aatgcagctt gttgcgacat cgccgggtca gagaagatct
    27721 ccgctttcgg ttcgtccgcc agaccagtga cgcgaactcc ggcgattacg tcgtcttctg
    27781 tagcatccgg gttacgaata gcttcaatat taagatacgg ttgatccggc ggaccagaga
    27841 acagtaactc acctttacgc acaatcagat cctgaccata ggcatggaag cgcccttcag
    27901 ggatgttgat ctgcccgttc aggcccagcc cttgtttgtc ctgaacgacg ttaagatcgc
    27961 ccgtcagccg cgctttcagg ccaaaggcgt caatgcgcac attgttgcca acgtggacaa
    28021 tcaggttact gttaatcgga atcgacgccg ttttcggctc ttccggttgc aggttatcgt
    28081 taagcatcac cacatcgctg gagacgccta ctgcgctttc cggcagatcg tgcaccacga
    28141 tgcgcgccca cggaacatcc acgcgaccat cgagggtaaa caggtttggt gtagcctcga
    28201 agacaacatc tggcgataca tccattcgta ccatcggcgg cacggtgatc cgcactttac
    28261 tgcctttcgc cgttacccgc gcccgccagt tttcaatttg gctccagtcg gcatcaccgt
    28321 tcaggtagat ttcaccctgc tgggtccgta ctgtaccggc aagcgtcgag cgcataccat
    28381 taaagttgac cgcaagctgg ctcggttgca tatcaaacgg cataaagttg ccgtcgatat
    28441 ccacacccgt aacctgaagc tgaccaaaca actgcgggct ttgcacatca ccacccagac
    28501 gcaagttggc actcaccatc cccgctgctt tttccccacg ggtaaagatg gggtttatca
    28561 tcgcaaggtt gaagttacgg atattgacgt tgccaccaag attacggcgg ccttgcggat
    28621 cggtcacctg cacctgtcca tcaaactggc cgttattggt caggcggatg gtccagccca
    28681 attcggcacg gttgttacgc aattccgccg tcagattcag tgtctgaaac gccacgggca
    28741 gcgccgcatc gttgacggtt tgcgttacct gcacgttacg ccccgaaagg gtgatactgc
    28801 cctgcggcag cccctctttg gtggtgtccc aggcaacgtc cgctttaccc gtgaagatac
    28861 cgctggcctg agtggtttct ggcataaatg gtttcagcat ggccaggtcg aagcggttga
    28921 gattcaccac cgcacgccct tcggcacccg catcgatagt ttgcggcacg cacagttccg
    28981 cattcgggtt aagccagcag tgtggcccga tgctgatttt ttgctcctta ttgcggtaat
    29041 ccagcgcaat atcgcgggtc agcgaccacg ggccgaccgg cgtctggaag cgggtattgc
    29101 taagagttcc cttccagcgt tcttctttgc gatcaaaact tcctgccaga ttaagctgcc
    29161 cggagacagg ctcgccctga atccgcaact gtaactcgtg ctgtttttcg ctgcctttgg
    29221 cattcagggt gacgaggttg atatttacat ccggctgcga aatttgctca acgcgtacgt
    29281 cgagtttccc ggcgatctga tcggtggatt taatgtcgcc ttccacgcga acctgcgcca
    29341 cggaaagttc ctgccagcgc aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt
    29401 ccaccgtgcc gcgtactttc accagccctt tcgctgtacc gccaagcccc ggcagcgcgt
    29461 tatccagccc cggcgcgttg atggtggcat caagattgag atcttttacc cccagctcgc
    29521 ctttcacttc ggcactgttt ggccccagtt ccagatgaag ccctgggatc atccactgca
    29581 tataactgtt gcctttcagc gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg
    29641 tcagcttcag ttctggcacc tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt
    29701 taatcaagcc attgagtttc gacggccact ctgggatctc tttggcggtg ttaatgccgt
    29761 taagcgttag ctcaccgcgc caactaatgg cctgctgcca gtcgagcaac gccttgagtt
    29821 cagttttccc ttccagcgcc gcgacggtga gtttgtcgag attgacctgc tgttcattac
    29881 ctttggcatc aagggtaatg gtggctggcg ggatctccag tcccttcact gccgtacgca
    29941 tagagagcgt gtaatcggtc attttgccgg taagtttcag tttcagatca tccgcctgat
    30001 actgcttctc accagtgaac ggccagtaaa tctgtttgct gttcacttcc acgttgagcg
    30061 gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa
    30121 gattaacgcc aatctccagc tgttcgcgca gcgcgccgcc cactttcagc ttcacttttt
    30181 cacctttcaa cggctccacg ttcagtgtgc tgttgagggt gatatccacc ggccagttgt
    30241 ctgacagctg cgccgtgccg ctggcgttga cgctcccctg gttggaatcg atatccaggg
    30301 cgtccagttt agtattgccg tcaatgctgc tcactttcag cagcatggtg cgcacggtga
    30361 tgtccgtgtc gcccgtcacg cgcaactgct cgcccttaaa ctcttcaatg ttcaggttaa
    30421 gcggcaaatg cacgtcggtc atttccggca ataccgggcg agaaaagaga tctttcagcg
    30481 tttcgccgag cggcttttcc tccggctgcg gattttcaat tttcggttcg accacttctt
    30541 cctgcgccac ttccgccact ttcggcagag caatcagcag gcctttcagc gacgtcggtt
    30601 tcagggtcag ggttttctcc tgccagttca gaccggaggt gaagtccatc accgataccg
    30661 tggtgtcatc aatcttgatg ttgacgttat ccagcgccac ccgtgtcagg gtgatgggat
    30721 acggcgtgga gagatccagc ggaccactat cttcctcttc ttcaacctgt tcagaagggg
    30781 gcattttttt actgtcgatg ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa
    30841 cgctactgtt ccaaagacaa tccagcccga ccgccagatg cagattgccc gcttttaccg
    30901 ccacgcctgg ctgctcataa cgaacgtcag acaaggtgag atcgcgccag ccgccggtga
    30961 ccttgccaat atccagtcct ggcacccagc gatccgccgc tttaaatacc agatgcaggc
    31021 cgctggtggt gcccaccaga aacgccaccg atcccagcaa cagtaagata acgataacca
    31081 cgccgaggct gatttttttc cataaactca taattctggc cccagaccga tgtaaaactg
    31141 taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac
    31201 cggcgattcc cagcgtacgc cgaccccggt accggtttta aagtcgctgc ggcgaatatc
    31261 gcttaccgct tcgccactat cgacaaacac cgcgccccac cattttccgg tcacgttgta
    31321 ctggtattcc agcgatccgg ttatcaactt cgaggcccct ttcaggtcac cgttggcgta
    31381 tttcggggcg atagatttgt atttgtagcc gcgaatactg cgatcgcccc cggcgaagaa
    31441 acgcagatcc ggcggtactt tgtcgaaatc accggtttca atccagccca gcgtgccgcg
    31501 tgtaacaaaa cgatggcgat cgtacagtgt gcggatccag acgttttgtg cctggaaaac
    31561 ggagaaatcg acatctgagc cccaggccgt gttggagtag tcgatagagt agcgttgcga
    31621 gtcgccccag gttggcatca agccaccacg agaacgcgtg cggctaatca tcaccccagg
    31681 ataaaacagc atcgtggtgt tggtaatttc accctgagta aagtggtcga gactccagcg
    31741 caggttaatg gcacgctgcc agccgctgga gagatcccag tagcgagaag ccaccagcgt
    31801 agtggagtca gattcggtat cgttcaggtc agtgcgctta aaaccgccct gcaccaaata
    31861 atattgttcc agtggattct tcagcagtgg cattttatag ctgaagtcga gggtctgttc
    31921 cggcgcggaa atactggtac tggtggtcag actgtgacca taagagttca tccacggctt
    31981 tttccacgtc gctttcacgc gcggtcccac gtccgtagag taaccgaccc cggtttcgat
    32041 ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc
    32101 tttatcaaat tgtggagcca ccaccaccga gttaaaccag ccggtagcag aaagtcggcg
    32161 gttcagttcc gccagatctt tcgattcgta ctcatcgccc tctttaaacg gcaccagatt
    32221 ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg
    32281 ttcgccactg ttataatcaa tatcccagaa ggctttatgc aggccgagcg caatgcccag
    32341 ctgcgctttg gtaaattcgc tatcgaaata acctttacgc aacgcaatgc tggttaagga
    32401 ctttttgaaa ttttcataat cgccctggtt cagtaccgta ccaatagccg ggcgagtatc
    32461 gagcaatttc aaatagtctt tatcggtccg cgcgccgccg cgcaatacca catcggtgcc
    32521 gccaattaac accggcacgc ctggcgtgac tttggcgatc aatacctgcc gccctttctt
    32581 tggcggtgga cggagatcaa attcaatggt cggctggtaa taacccagcg ctttcagacc
    32641 ttcgcggatg gcatcatcga cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact
    32701 ttcaatcgta gaaagctgcg cgcgaacgtt cttttccagc tgtcccgata acccctcgac
    32761 ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag cagagtaagc ttacacagca
    32821 taactgtcgg atatagcgca cattttctcc tgaatatcct tttttcctgc ccctggaacg
    32881 ccgttaaacg gcttaacaaa aatccagtaa tatggattaa aaagcagact aaaccccaaa
    32941 tatttcttat gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct
    33001 tgttacaacc ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga
    33061 taaaaagcat ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt
    33121 agccacgctg catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat
    33181 tgccattttt gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg
    33241 cgtttacagc accgccgcag gctataccgg aggctatacg ccaaatccga cttatcggga
    33301 agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc attgtttacg atccttccgt
    33361 catcagctat gagcagttgc tacaggtatt ttgggagaat cacgatcccg cccagggaat
    33421 gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgaccccaga
    33481 acaggatgcc gcagctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga
    33541 tgatgatcgt cacatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga
    33601 tgaccaccag caatatctgc ataaaaaccc gtatggttac tgtggaattg gcggaattgg
    33661 cgtctgtctg ccaccggaag catagcgtta cgggtacaaa tgtagattgt tgataaagtg
    33721 cgctttattt atgccagatg cggcgtaaac gccttaccag gcctacaaaa tcgtccaaat
    33781 tcaatatatt gcaaggactg cgtaggcctg attggcatag cgcatcaggc agtattgcat
    33841 ttatcagctg tctgaatttt aaccctctgg cgactttaca gtaccttacg ctatactagc
    33901 cactgaaaat gccggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt
    33961 aatcaacttc cccttccgag gatctggcct gaaaggtcgg ataagatatg ttaaacagta
    34021 ttttagtcat actctgcttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc
    34081 ttgccgcctc acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc
    34141 aacgcgttct gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc
    34201 tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc
    34261 acagcctgtt ctcccgctat atgtcggcag aactctctga gcaactgagc tttattctct
    34321 ctttctcgtt agtgactggc atgtttatcc tgtttgcgga tttaactccg aaacgcatcg
    34381 gtatgattgc gccagaagcg gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt
    34441 acgtttgcac cccgctggtg tggttcttca acggcctggc gaacatgatc ttccgtattt
    34501 tcaaactgcc aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg
    34561 aagccggtgc gctggcgggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct
    34621 ttgagctgga atcccgtacc gttccgtctt caatgacacc gcgtgaaaac gtgatttggt
    34681 ttgatctcca cgaagatgag caaagtctga agaataaggt ggcggaacat ccgcactcta
    34741 agttcctcgt ctgtaatgaa gatattgacc acatcatcgg ttatgtcgat tctaaagacc
    34801 tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca
    34861 acacgctgat tgtaccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg
    34921 caggtgaaga cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg atcatcaccc
    34981 tcaacgacgt gatgaccacg ctgatgggcg atctggtcgg tcaggggctg gaagagcaga
    35041 ttgtcgcccg tgatgagaac tcatggctga ttgacggcgg caccccgatt gacgacgtca
    35101 tgcgcgtgct ggatattgac gagttcccgc agtcgggcaa ctacgaaacc atcggcggct
    35161 ttatgatgtt tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct
    35221 acaaatttga agtggtggat atcgataact accgtatcga ccaactgctg gtgacccgga
    35281 tcgacagcaa ggccaccgcc ctttcgccaa aactgcctga cgctaaagat aaagaagaaa
    35341 gcgtcgcgta acccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    35401 atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga
    35461 catcactgac aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa
    35521 ttcaaaagcg ccaacgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt
    35581 cgccacctga agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt
    35641 acgcctgtgc ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt
    35701 gactatagac tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac
    35761 aattattcag aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca
    35821 tatgatttgt gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc
    35881 acatatgatt aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac
    35941 accatgaccc tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc
    36001 gcacatcagt tcgaaaccgg tcagcgagtg ccgccgattg gcattaccga tcggggcgag
    36061 ttggtgcttg ataaagatca gtttagctac aaaacctgga acagcgcgca gttagtggga
    36121 aaagtgcgag tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg
    36181 ctgattgaag cgattaaatc agcgaagtta ccgcacgatc gttaccagac caccaccatt
    36241 gttaacaccg acgacgcaat tccgggttcc ggcatgtttg tgcgcagcag tctggagagt
    36301 aataaaaagc tttatccctg gtcgcagttt attgttgata gcaatggcgt cgcacgcggt
    36361 gcctggcagc tggatgaaaa aagttccgct gtagtggtgc tggataaaga cggtcgcgtg
    36421 caatgggcca aagacggggc gcttactcag gaagaggtgc agcaagtgat ggacctgctg
    36481 cataaattaa ttaataaata gacactctga accccggatt caggaacgac tcgcgcggag
    36541 tgtaatccag cggtttaccc tgccagtcgt gaacgtgcgc tccggcagct gcagcgacag
    36601 catgtccagc ggcggtgtcc caaatattcg ttggtccgaa gcgcgggtac agctgcgcct
    36661 gtccttccgc caccaggcag aatttcagcg aagagccgat ggacgtggtc tgatgttcgc
    36721 caagctgttg cagatactct ttcagctccg catctgcatg ggaacggctg ataaccacca
    36781 gcggcgggcg cgcatcgcgg acctgaatct gcttgcgcac accgcactct tctttccacg
    36841 ctttgccttc tgccgcgctg tacattacgt tcattaccgg cgcatacacc acgcctaaaa
    36901 tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa ttcgccatta cgtttaataa
    36961 actctttagt accatccagt gggtctacca gccagtaacg ctgccagtgc tgacggactt
    37021 cccaaccggg aggatcttct tcagaaagga ccggaatatc cggtgtcagc gtacgtaaac
    37081 cgtccatgat aacggtgtgt gcggcaatat ccgctgccgt taccggagaa ttgtccgctt
    37141 tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg cataatggca tcgcctgcat
    37201 tccgtgcaag ctggcatact tgatctaaca tttctccacc tcgtctctgt gagcggtgtt
    37261 aacttattgt tttacttata ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc
    37321 attttcaaag cgtaaaattg tggcattctt cactgttcta taagtaagac gtttattctc
    37381 ccttttcttt cgtattcccg atgataaaag gatgtccctg atgattaagt ttagcgcaac
    37441 gctcctggcc acgctgattg ccgccagtgt gaatgcagcg acggtcgatc tgcgtatcat
    37501 ggaaaccact gatctgcata gcaacatgat ggatttcgat tattacaaag acaccgccac
    37561 ggaaaaattc ggactggtac gtacggcaag cctgattaac gatgcccgca atgaagtgaa
    37621 aaacagcgta ctggtcgata acggcgattt gattcagggg agtccactgg ccgattacat
    37681 gtcggcgaaa tgattaaaag caggtgatgt tcatccggtt tataaggcgc tgaatacgct
    37741 ggattacacg gtcggtacac tcggcaatca tgaatttaac tacggtctgg attacctgaa
    37801 aaatgcgctg gcgggagcga aattccctta tgtaaatgcc aacgtcattg acgccagaac
    37861 caaacagcca atgtttacac cgtatttaat taaagatacc gaagtggtcg ataaagacgg
    37921 aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg ccgccgcaaa tcatgggctg
    37981 ggataaagct aatttatccg gaaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa
    38041 atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcgtt gtcgttctgg cgcattccgg
    38101 gctatctgcc gatccgtata aagtgatggc ggaaaactca gtttattacc tcagtgaaat
    38161 tcctggcgtt aacgccatta tgtttggcca tgctcacgcc gttttcccag gtaaagattt
    38221 tgctgatatc gaaggggctg atatcgcgaa aggcacgctg aatggtgttc cggcggtaat
    38281 gccgggcatg tggggcgatc atcttggggt ggtcgactta caactcagta ataacagcgg
    38341 taaatggcag gtgacgcagg cgaaagcgga agcacggccg atttacgaca tcgccaataa
    38401 aaaatccctc gcggcggaag acagcaagct ggtagaaaca ctcaaagccg atcacgatgc
    38461 cacacgccag ttcgtcagca agccaatcgg taaatccgcc gacaatatgt atagctatct
    38521 ggcgctggtg caggacgatc cgaccgtgca ggtggtgaac aacgcgcaaa aagcgtatgt
    38581 cgagcattac attcagggcg atccggatct ggcaaaactg ccggtgcttt cagctgctgc
    38641 accgtttaaa gttggtggtc gcaaaaatga tccggcaagc tatgtggagg tggaaaaagg
    38701 ccagttgacc ttccgtaatg ccgccgatct ttatctctac cccaatacgc tgattgtggt
    38761 gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc tccgccggac agtttaacca
    38821 gattgatcct gacaacacga aaccacagtc actcatcaac tgggatggtt tccgcaccta
    38881 taactttgat gtgattgatg gtgtgaatta tcagattgat gttacccagc ctgcccgtta
    38941 tgacggcgag tgccagatgg ttaatgccaa tgcggaaagg attaagaacc tgacctttaa
    39001 tggcaagccg attgatccga acgccatgtt cctggttgcc accaataact atcgcgctta
    39061 cggcggcaaa tttgccggta cgggcgacag ccatatcgct tttgcttcac cggatgagaa
    39121 ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg aaattcaccc
    39181 ggcggcagat aacaactggc gtttagcacc gatagctggc gataagaaac tggatatccg
    39241 tttcgaaacc tctccatcag ataaagccgc ggcgtttatt aaagagaaag ggcaatatcc
    39301 gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa
    39361 gtaatacact tctttttcgg cctataaatc atcaaccgca tccggcactt ttgtcggatg
    39421 cgatgctggc gcatcttatc cgccctacaa gtcatgaacc gtaggccgga taagcgcagc
    39481 cggcagttat gccgcacgtt catcccgcac cgccaacacc tcgggcaaat tcaactcaat
    39541 ccagtccgcc agtgcagcaa ccttttcgct cacctgctcg cccagcggcg tgaggctata
    39601 ttccacatgc ggcggcacca ccggatacgc gatacggtta agaaaaccat cctgttctaa
    39661 cgcctgtaac gactgcgcaa gcatcttttc gctcaccccg ccgattttgc gccgcaggtc
    39721 gctaaagcga tgagtacctt cgcgtagcgc caccagaatc aacacccccc aacggctggt
    39781 gacgtgtttc aacacctcgc gcgacgggca ctgttccgca aagagattac cctctttcag
    39841 ttgttgcgac aggctaacct gactcattcc atacttacct ttttgtgtgt acttactaaa
    39901 agtaagttta ggtgttagcg tatttaaaca caagacaaaa cgatggagac ttcccatgat
    39961 cgctattact ggtgccactg gccaacttgg tcactatgtt attgaatcct tgatgaaaac
    40021 ggttcctgcc agccaaatag tggctatcgt tcgtaatccg gcaaaagccc aggccctggc
    40081 agcacaaggc attaccgtgc gtcaggctga ctacggcgat gaagccgcac tgacatctgc
    40141 acttcaggga gtggaaaaac tactgctgat ctcttccagc gaagtgggtc aacgtgcccc
    40201 gcagcatcgt aatgttatta atgccgcaaa ggcggctggc gtgaaattta tcgcttatac
    40261 cagcctgcta catgcagata cctccccgct cggcctcgcc gatgagcaca tcgagacgga
    40321 gaaaatgctg gctgattctg gcatcgttta caccctgctg cgcaacggct ggtacagcga
    40381 aaactacctc gccagcgccc cggcagcact agaacacggc gtatttatcg gtgcggcggg
    40441 cgatggcaaa atcgcctcag caacgcgggc agattatgcg gcagctgcgg cacgcgtgat
    40501 tagcgaagcc ggtcacgaag gcaaggttta cgaactggcg ggcgatagtg cctggacgtt
    40561 gacacagtta gcggcagagc tgaccaaaca gagcggcaaa caggttacct atcaaaatct
    40621 gagcgaagcc gatttcgccg cggcactgaa aagcgtcgga ctgcccgacg gactggcgga
    40681 tatgctggcg gattctgacg ttggcgcatc gaaaggcggt ctgtttgatg acagcaaaac
    40741 acttagcaaa ttgattggcc acccaacgac aacgttagcc gaaagcgtaa gccatctttt
    40801 taatgttaat aactagttaa ttaaagtggc atcctcccgc atcctctctg ataatgacgg
    40861 gatgccggga gcaatcatgt ctgcttcctg aactttcttc tgacagatca atggatgcca
    40921 gtaatgatta gcggcgtgct gtacgccctg ttagcagggt tgatgtgggg gcttattttt
    40981 gtcgggccgt tgatcgtgcc ggaatacccg gcgatgttgc agtcgatggg gcgttatctg
    41041 gcgttagggt taattgcgct gcccattgcc tggctgggac gcgtgcgtct gcgtcagttg
    41101 gcgcgtcgcg actggcttac cgccttgatg ctcactatga tgggcaacct catctattac
    41161 ttctgccttg ccagtgccat tcaacgtact ggcgcgcctg tttccacgat gattatcggc
    41221 accctgccgg tggtcattcc cgtcttcgcc aatctgcttt atagccagcg cgacggcaaa
    41281 ctcgcgtggg gaaaactcgc cccggcactg atttgtattg gcatcggcct ggcgtgtgtg
    41341 aatattgctg agttaaacca cggactcccc aattttgact gggcacgtta tacctctggc
    41401 atcgtgctgg cgttagtttc cgtggtctgc tgggcgtggt atgccctgcg caacgcccgc
    41461 tggctgcggg aaaatcccga caaacatcca atgatgtggg cgacggcgca ggcactggtc
    41521 acgctgccgg tttctctcat cggctatctc gtcgcttgtt actggctgaa tacgcaaacg
    41581 ccggacttct ccctaccctt tggcccccgt ccgctggtgt ttattagtct gatgattgcg
    41641 atagccgtgc tttgctcatg ggttggcgca ctctgctgga acgtcgccag ccagcgatta
    41701 ccgacagtga ttctcgggcc gctgattgtt ttcgaaacgc tggcaggttt gctgtacacc
    41761 tttttactcc gccagcaaat gccgccgcta atgacgctga gcggtatcgc gctgttagtg
    41821 attggcgtgg tcatcgcggt cagagcaaaa ccagaaaaac ctttaactga atctgtctca
    41881 gaaagttgac acgctggcag tgagttaaat aagtctctgc tacgtaaggg ttatagc
//
LOCUS       DAZFRG010000009        41314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFRG010000009 DAZFRG010000000
VERSION     DAZFRG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.009"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(13..188)
                     /locus_tag="ACHKUC_000343"
                     /pseudo
     CDS             complement(13..188)
                     /locus_tag="ACHKUC_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(399..1244)
                     /gene="araC"
                     /locus_tag="ACHKUC_000344"
     CDS             complement(399..1244)
                     /gene="araC"
                     /locus_tag="ACHKUC_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO6616671.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHSEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            1583..3283
                     /gene="araB"
                     /locus_tag="ACHKUC_000345"
     CDS             1583..3283
                     /gene="araB"
                     /locus_tag="ACHKUC_000345"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO6616672.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQHAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKDQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEKHYLPTSAPAQAAQAVPTL
                     "
     gene            3294..4796
                     /gene="araA"
                     /locus_tag="ACHKUC_000346"
     CDS             3294..4796
                     /gene="araA"
                     /locus_tag="ACHKUC_000346"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO6616673.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            5081..5776
                     /gene="araD"
                     /locus_tag="ACHKUC_000347"
     CDS             5081..5776
                     /gene="araD"
                     /locus_tag="ACHKUC_000347"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6616674.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            5851..8202
                     /gene="polB"
                     /locus_tag="ACHKUC_000348"
     CDS             5851..8202
                     /gene="polB"
                     /locus_tag="ACHKUC_000348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO6616675.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDSGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFCLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGLKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            8367..11273
                     /gene="rapA"
                     /locus_tag="ACHKUC_000349"
     CDS             8367..11273
                     /gene="rapA"
                     /locus_tag="ACHKUC_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO6616676.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            11285..11944
                     /gene="rluA"
                     /locus_tag="ACHKUC_000350"
     CDS             11285..11944
                     /gene="rluA"
                     /locus_tag="ACHKUC_000350"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO6616677.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(12062..12877)
                     /gene="djlA"
                     /locus_tag="ACHKUC_000351"
     CDS             complement(12062..12877)
                     /gene="djlA"
                     /locus_tag="ACHKUC_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO6616678.1"
                     /translation="MQYWGKIIGVTVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            13132..15486
                     /gene="lptD"
                     /locus_tag="ACHKUC_000352"
     CDS             13132..15486
                     /gene="lptD"
                     /locus_tag="ACHKUC_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO6616679.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVAASNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15539..16825
                     /gene="surA"
                     /locus_tag="ACHKUC_000353"
     CDS             15539..16825
                     /gene="surA"
                     /locus_tag="ACHKUC_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO6616680.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            16825..17814
                     /gene="pdxA"
                     /locus_tag="ACHKUC_000354"
     CDS             16825..17814
                     /gene="pdxA"
                     /locus_tag="ACHKUC_000354"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO6616681.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            17811..18632
                     /gene="rsmA"
                     /locus_tag="ACHKUC_000355"
     CDS             17811..18632
                     /gene="rsmA"
                     /locus_tag="ACHKUC_000355"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO6616682.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            18635..19012
                     /gene="apaG"
                     /locus_tag="ACHKUC_000356"
     CDS             18635..19012
                     /gene="apaG"
                     /locus_tag="ACHKUC_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO6616683.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            19019..19867
                     /gene="apaH"
                     /locus_tag="ACHKUC_000357"
     CDS             19019..19867
                     /gene="apaH"
                     /locus_tag="ACHKUC_000357"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO6616684.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(19864..20178)
                     /locus_tag="ACHKUC_000358"
     CDS             complement(19864..20178)
                     /locus_tag="ACHKUC_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO6616685.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(20181..20414)
                     /locus_tag="ACHKUC_000359"
     CDS             complement(20181..20414)
                     /locus_tag="ACHKUC_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO6616686.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENTEAIAALNQLADETGCFSDEYRSF"
     gene            complement(20500..20979)
                     /gene="folA"
                     /locus_tag="ACHKUC_000360"
     CDS             complement(20500..20979)
                     /gene="folA"
                     /locus_tag="ACHKUC_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO6616687.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(21171..23033)
                     /gene="kefC"
                     /locus_tag="ACHKUC_000361"
     CDS             complement(21171..23033)
                     /gene="kefC"
                     /locus_tag="ACHKUC_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO6616688.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASNASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(23026..23556)
                     /gene="kefF"
                     /locus_tag="ACHKUC_000362"
     CDS             complement(23026..23556)
                     /gene="kefF"
                     /locus_tag="ACHKUC_000362"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO6616689.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGTHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(23664..24995)
                     /gene="yaaU"
                     /locus_tag="ACHKUC_000363"
     CDS             complement(23664..24995)
                     /gene="yaaU"
                     /locus_tag="ACHKUC_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6616690.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(25054..25341)
                     /gene="fixX"
                     /locus_tag="ACHKUC_000364"
     CDS             complement(25054..25341)
                     /gene="fixX"
                     /locus_tag="ACHKUC_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO6616691.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(25338..26624)
                     /gene="fixC"
                     /locus_tag="ACHKUC_000365"
     CDS             complement(25338..26624)
                     /gene="fixC"
                     /locus_tag="ACHKUC_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6616692.1"
                     /translation="MFEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(26675..27616)
                     /gene="fixB"
                     /locus_tag="ACHKUC_000366"
     CDS             complement(26675..27616)
                     /gene="fixB"
                     /locus_tag="ACHKUC_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6616693.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(27631..28401)
                     /gene="fixA"
                     /locus_tag="ACHKUC_000367"
     CDS             complement(27631..28401)
                     /gene="fixA"
                     /locus_tag="ACHKUC_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO6616694.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAVEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            28874..30388
                     /gene="caiT"
                     /locus_tag="ACHKUC_000368"
     CDS             28874..30388
                     /gene="caiT"
                     /locus_tag="ACHKUC_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO6616695.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            30419..31561
                     /gene="caiA"
                     /locus_tag="ACHKUC_000369"
     CDS             30419..31561
                     /gene="caiA"
                     /locus_tag="ACHKUC_000369"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO6616696.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            31679..32896
                     /gene="caiB"
                     /locus_tag="ACHKUC_000370"
     CDS             31679..32896
                     /gene="caiB"
                     /locus_tag="ACHKUC_000370"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO6616697.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            32969..34522
                     /gene="caiC"
                     /locus_tag="ACHKUC_000371"
     CDS             32969..34522
                     /gene="caiC"
                     /locus_tag="ACHKUC_000371"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO6616698.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPLSANDQQHRLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            34631..35416
                     /gene="caiD"
                     /locus_tag="ACHKUC_000372"
     CDS             34631..35416
                     /gene="caiD"
                     /locus_tag="ACHKUC_000372"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO6616699.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            35422..36012
                     /gene="caiE"
                     /locus_tag="ACHKUC_000373"
     CDS             35422..36012
                     /gene="caiE"
                     /locus_tag="ACHKUC_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO6616700.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHAALHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(36131..36526)
                     /gene="caiF"
                     /locus_tag="ACHKUC_000374"
     CDS             complement(36131..36526)
                     /gene="caiF"
                     /locus_tag="ACHKUC_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO6616701.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(36787..40005)
                     /gene="carB"
                     /locus_tag="ACHKUC_000375"
     CDS             complement(36787..40005)
                     /gene="carB"
                     /locus_tag="ACHKUC_000375"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO6616702.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLCQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECESNPSDRDKIMVLGG
                     GPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVE
                     RLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTA
                     VSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAY
                     TLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKAT
                     GVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTG
                     EVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDA
                     THGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRR
                     SALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(40023..41171)
                     /gene="carA"
                     /locus_tag="ACHKUC_000376"
     CDS             complement(40023..41171)
                     /gene="carA"
                     /locus_tag="ACHKUC_000376"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO6616703.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKCNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTIKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         9888 a        11195 c        10802 g         9429 t
ORIGIN      
        1 cgcctgatgc cgctacactt ttgccagacc atgttctcct ctgagggagt aactggcgtt
       61 tttcatagaa tgccgtactt ttttcaacaa ctgccacatc gaacggcatt tatggtggcg
      121 tgaagtgcct gccataaccg ctcaatgtgg tgttcctacg atgaacaaac cgcttgatat
      181 attctcctga tgcaaccgga caatatgttc atgatgggta tcccgataca taagccttcg
      241 ttttattttt acaaaaagaa atcgttgtcg atattttatc aagaaaaaaa taattcaggt
      301 aaaacgattc aactaaaaat aactaaaatt aatcccccgt gaaatatata aataacgtca
      361 gatgaagaaa gcaagggggt tccccccctt ctttcttatt attcacaacc tgccctaaac
      421 tcgctcggac tcgccccggt gcatttttta aatactcgcg agaaatagag ttgatcatca
      481 aaaccgacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
      541 gcctgactga tgcgctggtc ctcgcgccag cttaatacgc taatccctaa ctgctggcgg
      601 aacaaatgcg acagacgcga cggcgacagg cagacatgct gtgcgacgct ggcgatatca
      661 aaattactgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
      721 atcggcggat gaagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
      781 agatttatcg ccagcaattc cgaatagcgc ccttcccctt gtccggcatt aatgatttgc
      841 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa accggtattg
      901 gcaaatatcg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
      961 cactggtgat accattcgtg agcctccgaa tgacgaccgt agtgatgaat ctctccaggc
     1021 gggaacagca aaatatcacc cggtcggcag acaaattctc gtccctgatt tttcaccacc
     1081 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     1141 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcgtta
     1201 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     1261 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     1321 tactggctct tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa
     1381 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     1441 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     1501 agattagcgg atccagcctg acgctttttt tcgcaactct ctactgtttc tccatacccg
     1561 tttttttgga tggagtgaaa cgatggcgat tgcaattggc ctcgattttg gcagtgattc
     1621 tgtgcgagct ttggcggtgg actgcgccac cggtgaagag atcgccacca gcgtagagtg
     1681 gtatccccgt tggcagaacg ggcaattttg tgatgccccg aataaccagt tccgtcatca
     1741 tccgcgtgac tacattgagt caatggaagc ggcgctgaaa actgtgcttg cagagcttag
     1801 cgtcgaacag cacgcagctg tggtcgggat tggcgttgac acaaccggct cgacgcccgc
     1861 accgattgac gccgacggta acgtgctggc gctgcgcccg gagtttgccg aaaacccgaa
     1921 cgcgatgttc gtattgtgga aagaccacac cgcggttgaa gaagcggaag agataacccg
     1981 cttatgtcac acgccgggca acgttgacta ctcccgctat attggcggta tttattccag
     2041 cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg tggcgcaatc
     2101 tgccgcatcg tggattgagc tgtgcgactg ggtaccagct ctgctttccg gtaccacccg
     2161 cccgcaggat attcgtcgcg gacgttgcag cgccggacat aagtcgctat ggcatgaaag
     2221 ctggggcggc ctgccgccag ccagtttctt tgacgaactg gacccgatcc tcaatcgcca
     2281 tttgccttcc ccgctgttca ctgatacctg gactgccgat attccggtgg gcacgttatg
     2341 tccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg gcggcgcgtt
     2401 tgactgccat atgggcgcag ttggtgcagg cgcacagcct aacgcactgg taaaagttat
     2461 cggtacttcg acctgcgaca ttctgattgc cgacaaacag agcgttggcg agcgggcagt
     2521 gaaaggtatt tgcggtcagg ttgatggcag cgtggtgcct ggatttatcg gtctggaagc
     2581 aggccaatcg gcgtttggcg atatctacgc ctggttcggt cgcgtactcg gctggccgct
     2641 ggaacagctt gccgcccagc atccggaact gaaagatcaa atcaacgcca gccagaaaca
     2701 actgcttccg gcgctgaccg aagcatgggc taaaaacccg tctctggatc acctgccggt
     2761 agtactcgac tggtttaacg gtcgtcgcac gccaaacgct aaccaacgcc tgaaaggggt
     2821 gattaccgat ctgaacctcg ctaccgacgc tccgctactg ttcggcggtt taattgccgc
     2881 caccgccttt ggcgcacgcg caattatgga gtgcttcacc gatcagggga tcgccgtcaa
     2941 taacgtgatg gcactgggcg gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg
     3001 cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc
     3061 ggcgattttc gctgccgtcg ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa
     3121 aatggccagt gcggtagaga aaaccctgca accgtgcagc gaacaggcac aacgctttga
     3181 acagctttat cgccgctatc agcaatgggc gatgagcgcc gaaaaacact atcttccaac
     3241 ttccgccccg gcacaggctg cccaggccgt tccgactcta taaggacacg acaatgacga
     3301 tttttgataa ttatgaagtg tggtttgtaa ttggcagcca acatctgtat ggcccggaaa
     3361 ccctgcgtca ggtcacccaa catgccgagc acgtcgttaa tgcgctgaat acggaagcga
     3421 aactgccctg caaactggtg ctgaaaccgc tgggcaccac gccggatgaa atcaccgcta
     3481 tttgccgtga cgcgaattac gacgatcgtt gcgctggcct ggtagtgtgg ctgcacactt
     3541 tctccccggc caaaatgtgg atcaacggcc tgactatgct caacaaaccg ttgctgcaat
     3601 tccacaccca gtttaacgcg gcgctgccgt gggacagtat cgatatggac tttatgaacc
     3661 tgaaccagac tgcacatggc ggtcgtgagt tcggcttcat cggcgcgcgt atgcgtcagc
     3721 aacatgccgt ggttaccggt cactggcagg ataaacaagc acatgagcgt attggctcct
     3781 ggatgcgtca ggcggtctct aaacaggata cccgtcatct gaaagtctgc cgttttggcg
     3841 ataacatgcg tgaagtggcg gtcaccgatg gtgataaagt tgccgcacag atcaagttcg
     3901 gtttctccgt caatacctgg gcggttggcg atctggtgca ggtggtgaac tccatcagcg
     3961 acggcgatgt taacgcgctg gtcgatgagt acgaaagctg ctacaccatg acgcctgcca
     4021 cacaaatcca cggcgaaaaa cgccagaacg tgctggaagc ggcgcgtatt gagctgggga
     4081 tgaagcgctt cctggaacaa ggtggcttcc acgcgttcac caccaccttt gaagatttgc
     4141 acggtctgaa acagcttcct ggtctggccg tacagcgtct gatgcagcag ggttacggct
     4201 ttgcgggcga aggcgactgg aaaactgccg ccctgcttcg catcatgaag gtgatgtcaa
     4261 ccggtctgca gggcggcacc tcctttatgg aggactacac ctatcacttc gagaaaggca
     4321 atgacctggt gctcggctcc catatgctgg aagtctgccc gtcgatcgcc gcagaagaga
     4381 aaccgatcct cgacgttcag catctcggta ttggtggtaa ggacgatcct gcccgcctga
     4441 ttttcaatac ccaaaccggc ccagcgattg tcgccagctt gattgatctc ggcgatcgtt
     4501 accgtctact ggttaactgc atcgacacgg tgaaaacacc gcactccctg ccgaaactgc
     4561 cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga
     4621 tcctcgctgg tggcgcgcac cataccgttt tcagccatgc gctgaacctc aacgatatgc
     4681 gccagttcgc cgagatgcac gacattgaaa tcacggtgat tgataacgac acccgcctgc
     4741 cagcgtttaa agacgcgctg cgctggaacg aagtgtatta cggatttcgt cgctaagtag
     4801 ccgaatcagg tatgtaacgc ctgatgcgac gctgacgcgt cttatcaggc ctacacgctg
     4861 cgattttgta ggccggataa gcaaagcgca tccggcattc accgcctgat gcggcgctgg
     4921 cgcgtcttat caggcctacg cgttccggat ttgtaggccg gataagcaaa gcgcatccgg
     4981 cattcaccgc ctgatgcgac gccgacgcgt cttatcaggc ctacacgctg cgattttgta
     5041 ggccggataa gcaaagcgca tccggcacga aggagtcaac atgttagaag atctcaaacg
     5101 ccaggtatta gaagccaacc tggcgctgcc aaaacacaac ctggtcacgc tcacctgggg
     5161 caatgtcagc gccgttgatc gcgggcgcgg cgtcctggtg atcaaacctt ccggcgtcga
     5221 ctacagcatc atgaccgccg acgatatggt cgtggttagc atcgaaaccg gtgaagtggt
     5281 tgaaggaacg aaaaagccct cctccgacac gccaactcac cggctgctct atcaggcatt
     5341 cccgtccatt ggcggcattg tgcacacaca ctcgcgccac gccaccatct gggcgcaggc
     5401 gggtcagtcg attccagcaa ccggtaccac ccacgccgac tatttctacg gcaccattcc
     5461 ctgcacccgc aaaatgaccg acgcagaaat caacggtgaa tatgagtggg aaaccggtaa
     5521 cgtcatcgta gaaaccttcg aaaaacaggg tatcgatgcg gcgcaaatgc ccggcgtcct
     5581 ggtccattct cacggcccat ttgcatgggg aaaaaatgcc gaagatgcag tgcataacgc
     5641 catcgtgctg gaagaggtcg cttatatggg gatattctgc cgtcagttag cgccgcagtt
     5701 accggatatg cagcaaacgc tgctggataa acactatctg cgtaagcatg gtgcgaaggc
     5761 atattacggg cagtaatgac tgtataaaac cacagccaat caaacgaaac caggctataa
     5821 tcaagcctgg ttttttattg gattttcagc gtggcgcagg caggttttat cttaacccga
     5881 cactggcggg acaccccaca aggtacagaa gtctccttct ggctggcgac ggacagcggg
     5941 ccgttgcagg ttacgcttgc accgcaagag tccgtggcgt ttattcccgc cgatcaggtt
     6001 ccccgcgccc agcatatttt gcagggtgag caaggctttt gcctgacgcc gctggcgtta
     6061 aaggattttc atcgccagcc ggtgtatggc ctttactgtc gcgcccatcg ccagttgatg
     6121 aattacgaaa agcgcctgcg tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg
     6181 ccagaacgct atctgatgga gcggtttatc acctcgccag tgtgggtcga gggtgatatg
     6241 cgcaacggcg ctatcgttaa tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc
     6301 aagtgggttt ctattgatat tgaaaccacc cgccacggtg agctgtactg catcggcctg
     6361 gaaggttgcg ggcagcgtat cgtttatatg ctggggccag agaatggcga cgcctccgcg
     6421 ctcgattttg aactggaata cgtcgccagc cgcccgctgc tactggaaaa acttaacgcc
     6481 tggtttgcga ctcatgatcc tgatgtgatc atcggttgga acgtggtgca gttcgatctg
     6541 cgaatgctgc aaaaacatgc cgagcgttac cgtattccgc tgcgtctggg gcgtgataac
     6601 agcgagctgg agtggcgcga gcacggcttg aaaaacggcg tcttttttgc tcaggccaaa
     6661 gggcgactga ttatcgacgg tatcgaggcg ctgaaatccg cgttctggaa tttctcttca
     6721 ttctcgctgg aaaccgtcgc tcaggagtta ttaggcgaag gaaaatctat cgataacccg
     6781 tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataagcctgc gctggcaact
     6841 tataacctga aagattgcga gctggtgacg cagatcttcc acaaaactga aatcatgccg
     6901 tttttgctcg aacgggcgac ggtgaacggc ctgccggtag atcgacacgg cggttcggtg
     6961 gcagcgtttg gtcatctcta ttttccccgt atgcatcgcg ctggttatgt cgcgcctaat
     7021 ctcggcgaag tgccgccgca cgccagccct ggcggctacg tgatggattc gcgacctggg
     7081 ctttatgatt cggtgctggt gctggactac aaaagcctgt acccgtcgat catccgcacc
     7141 tttctgattg atcccgtcgg gctggtggaa ggcatggcgc agcctgatcc agagcacagt
     7201 accgaaggtt ttctcgatgc ctggttctca cgggagaaac attgcctgcc ggagattgtg
     7261 actaacatct ggcacggacg cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag
     7321 gcgctgaaga tcattatgaa tgccttttat ggtgtgctcg gcaccaccgc ctgccgtttc
     7381 ttcgatccac gcctggcatc gtcgatcacc atgcgtggcc atcagatcat gcggcaaacc
     7441 aaagcgttga ttgaagcgca gggctacgac gtgatctacg gcgataccga ctcaacgttt
     7501 gtctggctga aaggtgcaca ttcagaagaa gaagcggcga aaatcggtcg tgcactggtg
     7561 cagcacgtta acgcctggtg ggcagaaacg ctgcaaaaac aacggctgac cagcgcactg
     7621 gaactggagt atgaaaccca tttctgccgt tttctgatgc caaccattcg cggagccgat
     7681 accggtagta aaaagcgtta tgccgggctg attcaggagg gcgataaaca gcggatggtg
     7741 tttaaagggc tggaaaccgt gcgcaccgac tggacgccgc tggcccagca gtttcagcag
     7801 gagctttacc tgcgtatctt ccgtaacgag ccatatcagg aatacgtccg cgaaaccatc
     7861 gacaaactga tggcgggtga actggatgcg cggctggttt atcgtaaacg ccttcgccgt
     7921 ccgctgagcg agtatcagcg taatgtgccg cctcatgtac gcgccgctcg ccttgccgat
     7981 gaagaaaacc ataagcgcgg tcgccccttg caatatcaga accgcggcac cattaagtac
     8041 gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccact ggattacgaa
     8101 cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt tattgaggat
     8161 aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa aagagttcgc
     8221 cagataccat tttgatgcgt gacgaatgct ttgccatcca gtaccatagc gccctttcca
     8281 ttcctggacc tgaataacac cactacctca taagcacggt agcgggtggt tattgcctgc
     8341 aattaaagat atagagccga acacatatgc cttttacact tggtcaacgc tggatcagcg
     8401 atacagaaag cgaattggga cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt
     8461 tacttttccc atctactggt gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc
     8521 gcgtgatgtt caaccctggt gataccatta ccagccatga cggctggcag atgcaagtcg
     8581 aagaagtaaa agaagaaaat ggcttgctga cctatatcgg tactcgcctt gatactgaag
     8641 agtccggcgt ggccctgcgt gaagttttcc ttgatagcaa actggtgttc agcaaaccgc
     8701 aggaccgtct gtttgccggg cagattgacc gtatggaccg ctttgcgctg cgttatcgcg
     8761 cgcgtaagta ttccagcgaa cagttccgta tgccgtacag cggcctgcgc ggtcagcgta
     8821 ccagcctgat cccgcaccag ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc
     8881 gcgtcctgtt ggctgacgaa gtgggtttag ggaaaaccat tgaagccggg atgatcctgc
     8941 atcagcaact gctctctggc gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac
     9001 agcatcagtg gctggtagaa atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg
     9061 atgagcgtta tgccgaagct cagcacgatg cttacaaccc gttcgatacc gagcagctgg
     9121 tgatttgctc gctggatttt gcccgtcgta gcaaacagcg tctggaacat ctctgtgaag
     9181 ccgagtggga tctactggtg gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc
     9241 cgagccgtga atatcaggcc attgaacaac tggcagagca cgtgccgggc gttctgctgc
     9301 tgaccgcaac cccggaacag ctggggatgg aaagccactt cgcccgtctg cgtctgctgg
     9361 acccgaaccg tttccacgac tttgcgcaat tcgttgaaga gcagaaaaat tatcgtccgg
     9421 ttgcggacgc cgttgccatg ctgctggcag gtaacaaact gagcaatgac gaactgaaca
     9481 tgctcggtga gatgatcggc gagcaggata tcgagccgct gttgcaagca gcaaacagcg
     9541 acagcgaaga tgcccagagc gcccgtcagg aactggtttc gatgctgatg gatcgccacg
     9601 gtaccagccg cgtgctgttc cgtaacaccc gtaacggtgt gaaaggcttc ccgaaacgcg
     9661 agctgcacac tattaagctg ccactgccga cgcagtatca gaccgctatt aaagtctccg
     9721 gcattatggg cgcacgtaaa agtgcggaag atcgcgctcg cgatatgctc tatccggagc
     9781 gtatttatca ggaatttgaa ggcgataacg ccacctggtg gaacttcgat ccgcgcgttg
     9841 agtggctgat gggctacctg accagccatc gctctcagaa agtgctggtg atctgcgcca
     9901 aagctgccac tgcgctgcaa ctggagcaag tactgcgcga acgtgaaggt attcgcgccg
     9961 cggtgttcca cgaaggcatg tcgataatcg aacgtgaccg cgctgccgcc tggtttgctg
    10021 aagaagacac cggtgcgcag gtactgctgt gctcggaaat cggttctgaa ggacgtaact
    10081 tccagttcgc cagccacatg gtgatgtttg acctgccatt caacccggat ctgctggagc
    10141 agcgtattgg gcgtctggat cgtatcggtc aggcgcacga tattcagatc catgtgcctt
    10201 atctggaaaa aacggctcag tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat
    10261 tcgagcacac ctgcccgacc ggacgcacta tctacgatag cgtgtacaac gatttgatta
    10321 actatctggc ctcgccggat gagaccgaag gctttgacga tctgatcaaa aactgccgcg
    10381 agcaacatga agcgctgaaa gcacagctgg aacaaggtcg tgaccgcctg ctggaaatcc
    10441 attccaacgg tggcgaaaaa gcccaggcac tggcagaaag cattgaagag caggatgacg
    10501 ataccaacct gatcgccttc gccatgaacc tgttcgatat tatcggtatc aatcaggacg
    10561 atcgcggcga caacatgatc gtgctgacgc cgtccgatca tatgctggtg ccggacttcc
    10621 ctggcctgtc ggaagatggc atcaccatca ccttcgaccg tgaagtggcg ctggcgcgtg
    10681 aagatgcaca gtttattacc tgggagcatc cgctgatccg caacggtctg gatctgatcc
    10741 tttctggcga taccggtagc agcacgattt cactgttgaa aaacaaagcg ttgccggtag
    10801 gtacgctgtt ggtggaactg atttatgtgg ttgaagccca ggctccgaag cagttgcagc
    10861 tcaaccgctt cctgccaccg acgccggtac gtatgctgct ggataaaaac ggcaacaacc
    10921 tggcggcgca ggtagagttt gaaaccttta accgccagct taacgcggtt aaccgtcaca
    10981 ccggcagcaa gctggttaac gcggtgcagc aggatgttca cgctatcctt cagctggggg
    11041 aagcgcagat cgagaaatct gcccgtgcac tgattgatgc agcgcgtaac gaagccgacg
    11101 aaaaactgtc tgccgagctg tctcgtctgg aagcgctgcg tgcagttaac ccgaacattc
    11161 gtgacgacga actgaccgcc attgagagca accgtcagca ggtaatggaa agcctggatc
    11221 aggcaggctg gcgtctggat gccctgcgtt tgatcgttgt aacgcatcag taacggagcc
    11281 gaaaatgggg atggaaaact acaatccacc gcaggaaccc tggttggtta tcctgtatca
    11341 ggatgaccat attatggtgg tcaacaagcc gagcggtttg ttgtctgtgc cgggtcgtct
    11401 ggaagagcac aaagacagcg tgatgacgcg cattcagcgt gattatccgc aggcagaatc
    11461 ggtgcatcgt ctggatatgg ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc
    11521 ggagcgggag ttaaaacgcc agttccgcga gcgcgagccg aaaaaacagt atgtggcccg
    11581 cgtctggggg catccatccc ctgcggaagg tctggtggat ctgccgctga tttgcgactg
    11641 gccaaaccgc ccgaaacaga aagtctgtta cgaaacaggt aaacctgcgc agacggagta
    11701 tgaagtggtg gaatatgcgg cggataacac ggcaagagtg gtgttaaaac cgattaccgg
    11761 gcgttcgcat cagctgcgtg tgcatatgct ggcgctgggt catccgattc tcggcgatcg
    11821 tttttatgca tcaccagaag cgagagcaat ggcaccgcgt ttgttgctac atgcagagat
    11881 gctgacgatt acccatccgg cgtatggtaa tagtatgaca tttaaagcac cagcggattt
    11941 ttaatagtca cgctggcgaa gttcggtagg ccggagaagg cgttcacgcc gcatccggca
    12001 attttgcgcc gattgcctga tgcgacgcgg gccgcgtctt atcaggccta cacgttgaaa
    12061 cttatttaaa ccctttctgc tgctttatca gctcataggc ctgctgaatt tcctgcgctt
    12121 tctgcttcgc catctccatc atctctggcg gcaaaccttt cgccaccaac ttatcgggat
    12181 ggtgttcact catcagctta cggtaggcac gtttgatggt ggtcgcatca tccgtcggct
    12241 tcacgcccag cacattacag gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt
    12301 taccaccgcc agattgctgc tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc
    12361 gcaaaaactg gtcaaactga gcgcgggaga tccctaattc ttcagcaatg acatacagca
    12421 ccgcccgttc attcgggtgc agtgaaccat cagcaaacgc cgcctgaatc tggatctcca
    12481 gaaacatacg aattaagtca aaacgaccaa agcagacact gcgaaactgg cgcatctttt
    12541 cgcgcagcgg gtaattgtct gattttccca cccggaacgc attttgcgcc gcagtacggg
    12601 aagcgccatg aaggttcatt cggtccatca actggctggc gatatgaata tcagcttccg
    12661 tgacgcgacc tttggatttg gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca
    12721 gcgcctgacg ctcacgctgg ttggcgaacc acgccatttt acggctacgg gctttatcaa
    12781 acatatggcc aattaacagg cccaatacta cgccccaaaa gccgccgccc attattaagg
    12841 ccacggtcac gccaatgatt tttccccaat actgcatata ttccccaaat cgacacacgg
    12901 atatcagggc tatctcccac aatataaagg cgcttttacc catttctggc tgcggtctat
    12961 tgtgagacat cgcctataat ttgcattatc atacctgtca ttcaatgccg tgcctaacac
    13021 cacagacgct attcggacag gattaacact agcgtagaga tgacgagtac gttagtctct
    13081 gaccgtttgt cacgcgcaac gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa
    13141 cgtatcccca ctctcctggc caccatgatt gccaccgccc tttatagtca acagggactg
    13201 gcagccgacc tcgcctcaca gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta
    13261 cagggcgata ccaatgactt acccgtgact atcaatgctg accacgcgaa aggggactac
    13321 ccggatgacg ccgtgtttac tggcagcgtg gatatcatgc agggtaacag ccgtctgcag
    13381 gccgacgaag tgcagctcca tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc
    13441 gttgatgcgc tcggtaatgt ccattacgac gataaccagg tgatcctcaa agggccgaaa
    13501 ggctgggcga atctgaacac caaagatacc aacgtctggg agggtgatta ccagatggtg
    13561 ggtcgccagg gccgcggtaa agcggacctg atgaaacaac gtggcgaaaa ccgctatacc
    13621 attctggata acggtagctt tacctcctgt ctgccgggtt ctgacacctg gagcgtggta
    13681 ggtagcgaaa ttattcatga ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt
    13741 aaggtgggtc cggtaccgat cttttatagc ccctatttgc agttgccggt gggtgacaaa
    13801 cgtcgctctg gtttcttgat cccgaacgcc aagtacacca ccaccaacta ctttgagttc
    13861 tacctgccat attactggaa catcgcgcca aatatggatg ccaccatcac gccgcattat
    13921 atgcatcgtc gtggcaatat catgtgggag aacgaattcc gctacctctc ccaggcgggc
    13981 gctggcttga tggaactgga ctatctgcct tcagataaag tctataaaga tgaacacccg
    14041 aacgatgaca gttcacgtcg ttggttattc tactggaacc actccggggt catggatcag
    14101 gtgtggcgtt tcaacgtcga ctacaccaag gtcagcgatc ctagctactt caatgatttc
    14161 gataacaagt acggttccag taccgacggc tacgcaacgc aaaaattcag cgttggctat
    14221 gcggtgcaaa acttcaatgc caccgtttca accaagcagt tccaggtctt cagcgagcag
    14281 aacaccagta gctactcggc agaaccgcag ttagacgtta actactatca aaacgatgtc
    14341 ggtcctttcg atacgcgaat ttacggccag gcggtgcact ttgttaacac cagagatgac
    14401 atgccagagg cgactcgtgt tcacctggaa ccgaccatca atctgccgct ctctaataac
    14461 tggggcagca tcaatactga agcgaagttg ctggcaaccc attatcagca aaccaatctt
    14521 gactggtata actccagaaa cacgaccagg ctggcagagt ccgctaaccg tgtaatgccg
    14581 cagttcaagg tagacggtag aatggtcttc gagcgtgata tggaaatgct ggcgccgggc
    14641 tatacccaga cactggaacc acgcgcgcag tatctgtatg tgccatatcg cgatcagagc
    14701 aaaatctata actacgactc gtcgctgctg caatctgact actccggcct gttccgggac
    14761 cggacttacg gcggtcttga ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca
    14821 tctcgcatat atgatgatgc tgccgttgaa cgttttaata tttccgttgg tcaaatctac
    14881 tatttcacgg agtctcgcac tggcgatgac aacataacat gggagaatga cgacaaaacg
    14941 ggctcactgg tatgggcagg cgatacttac tggcgcatct ctgatcgttg gggattgcgt
    15001 ggcgggattc agtacgatac acgtctggat aacgtagcgg ccagtaactc cagcattgaa
    15061 taccgtcggg atgaagaccg tctggtacag ctgaattacc gttacgccag cccggaatat
    15121 attcaggcaa cgctgcctaa gtactattcc actgctgagc aatataagaa tggtatttcg
    15181 caggtaggtg ccgtcgccag ctggccaatt gccgatcgtt ggtccattgt tggggcctac
    15241 tactacgaca ccaatgcgaa caagcaagcc gactctatgt taggtgtgca atacagctcc
    15301 tgctgctatg cgattcgcgt cggttacgag cggaagctga acggttggga taacgataaa
    15361 caacatgcgg tatatgacaa cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc
    15421 aactacggtc tgggtacgca agagatgctg cgttcgaaca ttctgccgta tcaaaacact
    15481 ttgtgatttg ttgatttacc acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat
    15541 gaagaactgg aaaacgctgc ttctcggtat cgccatgatc gcgaatacca gtttcgctgc
    15601 cccccaggta gtcgataaag tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga
    15661 cgttgatgga ttaatgcagt cggtaaaact gaacgctgct caggcaaggc agcaacttcc
    15721 tgatgacgcg acgctgcgcc accaaatcat ggaacgtttg atcatggatc aaatcatcct
    15781 gcagatgggg cagaaaatgg gagtgaaaat ctccgatgag cagctggatc aggcgattgc
    15841 taacatcgcg aaacagaaca acatgacgct ggatcagatg cgcagccgtc tggcttacga
    15901 tggtctgaac tacaacacct atcgtaacca gatccgcaaa gagatgatta tctctgaagt
    15961 gcgtaacaac gaggtgcgtc gtcgcatcac catcctgccg caggaagtcg aatccctggc
    16021 gcagcaagtg ggtaaccaaa acgacgccag cactgagctg aacctgagcc acatcctgat
    16081 cccactgccg gaaaacccga cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc
    16141 gattgtcgat caggcgcgta acggcgctga tttcggtaag ctggcgattg ctcattctgc
    16201 cgaccagcag gcgctgaacg gcggacagat gggctggggc cgtattcagg agttgccggg
    16261 gatcttcgcc caggcattaa gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc
    16321 cggcgttggc ttccatattc tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc
    16381 ggtgaccgaa gttcatgctc gccatattct gctgaaaccg tcgccgatca tgactgacga
    16441 acaggcccgc gtgaaactgg aacagattgc tgccgatatc aagagtggta aaacgacttt
    16501 tgctgccgca gcgaaagagt tctctcagga tccaggctct gctaaccagg gcggtgatct
    16561 cggctgggct acaccagata ttttcgatcc ggccttccgt gacgccctga cccgcctgaa
    16621 caaaggtcaa atgagtgcac cggttcactc ttcattcggc tggcatttaa tcgaactgct
    16681 ggatacccgt aatgtcgata aaaccgacgc ggcacagaaa gatcgtgcat accgcatgct
    16741 gatgaaccgt aagttctcgg aagaagcagc aagctggatg caggaacaac gtgccagcgc
    16801 ctacgttaaa atcctgagca actaatggtt aaaacccaac gtgttgtgat cactcccggc
    16861 gagcccgccg ggattggccc ggacttaatt gtccagcttg cacagcgtga gtggccagtc
    16921 gaactggttg tttgtgccga tgccactctc cttaccgacc gggcagcgat gctcggtttg
    16981 ccgctcaccc tccgtcctta ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca
    17041 ttaacgctac ttcctgtcgc gctacgtgaa tctgtcactg cggggcagtt agcggttgaa
    17101 aatgggcatt atgtggtgga aacgctggcg cgagcgtgtg atggctgtct gaacggtgaa
    17161 tttgctgcgc tgatcacagg ccccgtgcat aaaggcgtca ttaacgacgc aggcattccg
    17221 tttaccggtc ataccgagtt tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg
    17281 ctggcaaccg aagaacttcg cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc
    17341 gcagatgcta tcacccctgc gcttttgcac gaagtgattg ctattttgca tcacgatttg
    17401 cgcaccaaat ttggtattgc cgaaccgcgc attctggtct gcgggctgaa tccacacgcg
    17461 ggcgaaggcg gtcatatggg tacggaagag atagacacca ttattccggt gctcgacaag
    17521 ctgcgggcgc aggggatgaa actcaacggg ccgctgcctg ccgataccct gtttcagccg
    17581 aaatatcttg ataacgccga cgcggtgctg gcgatgtacc acgatcaggg tcttcccgtg
    17641 ctaaaatacc agggcttcgg gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc
    17701 acatcagtgg accacggtac cgcgcttgaa ctggcgggac gtggtaaagc cgatgtcggc
    17761 agttttatta cggcgcttaa tctcgccatc aaaatgattg ttaacaccca atgaataatc
    17821 gagtccacca gggccactta gcccgtaaac gcttcgggca aaactttctc aacgatcagt
    17881 tcgtgatcga cagcattgtt tctgccatta acccgcagaa gggccaggcg atggtcgaaa
    17941 tcggtcccgg tctggcggca ttgaccgaac cggtcggcga acgtctggac cagctgacgg
    18001 tcatcgaact tgaccgcgat ctggcggcac gtctgcaaac gcatccattc ttaggcccga
    18061 aactgacgat ttatcagcag gatgcgatga cctttaactt tggcgaactg gccgcgaaaa
    18121 tgggccagcc gctgcgtgtt ttcggcaacc tgccttataa catctccacg ccgttgatgt
    18181 tccatctgtt tagctatact gatgccattg ccgacatgca ctttatgttg caaaaagaag
    18241 tggtgaatcg tctggttgca ggaccgaaca gcaaggcgta tggtcgatta agcgtcatgg
    18301 cgcaatacta ttgcaacgtg atcccggtgc tggaagtacc gccgtcagcc tttacaccac
    18361 cacccaaagt ggattccgcc gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg
    18421 ttaaagatgt tcgcgtgttg agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa
    18481 ccattcgtaa cagcctcggc aacctgttta gcgtcgaggt gttaacggga atggggatcg
    18541 acccggcgat gcgagcggaa aatatctctg tcgcgcaata ttgccagatg gcgaactatc
    18601 tggcggagaa cgcgcctttg caggagagtt aacgatgatc aattcgcccc gagtgtgtat
    18661 tcaggttcaa agcgtctaca ttgaggcaca atcttcacct gataatgaac gttacgtttt
    18721 tgcttatacc gtaaccatac gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta
    18781 ctggctgatc accaatggca atggccgtga aaccgaagtc cagggcgaag gagtggttgg
    18841 cgtccagcca cttatcgcgc ctggcgaaga gtaccagtac accagcggtg caatcattga
    18901 aaccccgctg ggcaccatgc agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt
    18961 cagcatcgac attcccgtat tccgactcgc cgttcccaca ctcattcatt aaaagaatat
    19021 ggcgacatac cttattggcg acgttcatgg ttgttacgat gaactgatcg cattgctgca
    19081 taaagtagaa tttacccctg ggaaagatac cctctggctg acgggcgatc tggtcgcgcg
    19141 cggcccgggt tcgctggatg ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct
    19201 ggtgctgggt aatcacgatc tgcatctgct ggcggtattt gccgggatca gccgcaataa
    19261 accgaaagat cgcctgacac cgctgctgga agcgccggat gccgacgagc tgcttaactg
    19321 gctgcgtcgc cagcctttgc tgcaaatcga cgaagagaaa aagttggtga tggcccacgc
    19381 cgggatcacg ccgcagtggg atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc
    19441 ggtgctgtcg agtgactcct atcccttctt tcttgatgcc atgtacggcg atatgccaaa
    19501 taactggtca ccggaattgc gggggctggg aagactgcgt tttatcacca acgcctttac
    19561 ccgtatgcgt ttttgcttcc cgaacggtca actggatatg tacagcaaag aatcgccgga
    19621 agaggcccct gccccactga aaccgtggtt tgcgattcct ggccccgtcg ctgaagagta
    19681 caacatcgcc tttggtcact gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta
    19741 cgcgctggat accggctgct gctggggtgg tacattaacc tgcctgcgct gggaagataa
    19801 acagtatttt gtccagccgt cgaaccggca taaggatttg agtgagggag aggcggtagc
    19861 gtcttaaatc ccgtcgagca taaaatctaa tgctccttta atcaacgttc ggtgcgcgct
    19921 ggcatcgcaa aatttcgttc ccagcgcgtt aactggaaca gttgcagtct catgcgtcat
    19981 gagcacatat tctttgccgt ctaccagtaa aaggattggg ttaagccgtt ctggtgggcg
    20041 aatacggcta aatcgttcaa taggtgttaa tggaataacg atacggcgat taatctcacc
    20101 aataatgtcg ctggtaacat caataacaaa gggaaacgcg gcgttcctgc cgcgactgcg
    20161 gtataccgta aattgcatcg ctagaagctc cggtactcat cagagaaaca tccggtttca
    20221 tcagccaatt gatttaacgc agcgatagct tctgtgttct cttcacgcca acgtgttgct
    20281 gcatgttttt taagttcagc attgagcgca accgtaaggg tggcgctcat atttaagcct
    20341 gcatcacgag cgcgattgac taactcacgg tcgacggtga cggtcacact ttgtgtggta
    20401 cgttttgcag tcatgatctc gtgctcctgt acaatttata cgtctagtat acacggatat
    20461 ctgcacgtat cagtgatgcc ggaattctaa tatacgaaat taccgccgct ccagaatctc
    20521 aaagcaatag ctatgagagt tctgcgcatc agcgtcgtgg aattcgctga ataccgattc
    20581 ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttccg cgtcgatatg
    20641 cgtcagatac agtttttgcg ctttcggcag gaactgctca taaacgcgac cgccgccaat
    20701 caccatgatt tctggtacgt caccacacgc cgcaatggct tcatccaccg acttcaccca
    20761 cgttacgcga tcgtccgtac ccggttgact gctgagaata atatttttgc gtcctggcaa
    20821 cggacgaccg atggattccc aggtatggcg gcccataatc acgggtttat ttaaagtgtt
    20881 gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt ccatgccgat
    20941 aacgcgatct accgctaacg ccgcaatcag actgatcatt gagatttccc gataaaaaaa
    21001 attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga tgatgttgag
    21061 gaaaatttta tattctgctg gcgagtccac gctctctccc tggactcgcc gcattacaat
    21121 gaaacaaaaa caaacagtta gctgtaaagc gtgatttacg tcactcttta ttaggatgag
    21181 ggcttcgttt ccggttcatc cgccatattg ccagtatgtt tcccttcttc ggttccctgc
    21241 cagccatgtc gttgaattaa tgacagatgt tcacggtctt cggtaatgat ctcacttaac
    21301 atcgcgctgg tgcgtttata gaccgccgca cgggctttgg tgtcgttctc aaccattgcc
    21361 atctcttcca ccatctgaat attaaagcgg cggaacacat cggcgcgttc tcgcgcttca
    21421 tacggcccca gacctaaact ttccagtgcc agacgcccgg ttttcagcgc accttcgaag
    21481 gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg gtcgacatcg
    21541 cgggcgcggg caataatctg caaatgtggg aaatgttctt tcaccatctc cgtcagttgc
    21601 aggttggttt gcggatcgtc gatggcgtta atcagcactt ccgctttcgc cgctccggca
    21661 gattccagta aatccatccg cgtggcatcg ccataaaaca ctttcatacc gaatttacgc
    21721 agcgtttcga tatggtccgg gtcgtgatcg agcaccacca ttttcacccc gctggagagc
    21781 agtaaacgtc cggttatctg cccaaaacgc ccgaatccgg caatgattac ccgcggctgt
    21841 tcttcgtcga tctcatcggc ttcacgcgcc tcttcagtag aagattgctc aaggcgattg
    21901 aggatcacca gcagaatcgg cgttgccgcc atcgacagtg ccaccgccag ggtcagcgat
    21961 ttcgcccact ctggctccag cacattcgcc atctgcgccg caccaaatac tacaaaggca
    22021 aactcactgc cctgtcctaa cagcaccgca aaccagcgac gctgtttatt tggcacctgc
    22081 aacggtcggg caatcagcca cagcatggcg attttgatga tgaggaagcc gagcagcaaa
    22141 ataacaatgc gcaacggatt ttcaagcagc gtgccaaagt ctatcgacat gccaacgccg
    22201 atgaaaaaca gccccagcag caaaccttta aatggttcga tatcgctttc cagcgcatga
    22261 cggtattccg agcttgccag cagtacgccc gccagaaacg cgcccatcgc catcgacaag
    22321 ccgacctctt ccagcagcaa accaaagcca aacacgagga ataacgccac ggcactaaac
    22381 acttcccgca agccagagcg ggctacaaaa cgcagcgccg gacgcgtgac atagcgcccc
    22441 agcaatacca ccagcaccag cgcacccgcc acttttaacg ccgagagagc aaatgcgccc
    22501 atcgtcgtcg aggcattgct cgccgccagt agcgggatca tcgccaccag cgggatcgcc
    22561 gcgatatcct ggaacagcag caccgcaaag gcactgcggc ccatttgcgt caccatcaga
    22621 ttgcgttcat tcatcgcctg catggcaatt gccgtagagg agagcgccag cgtcatgccg
    22681 atcaattccg ctacctgcca gcgcagccca agtaacatgc agaacagccc cagcaggccg
    22741 ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag cttccacagc
    22801 ctttgtggat cgagttcgag gccgataata aacagcatca gcaccacgcc aatctcggca
    22861 aagtgcagaa tagattcggc atcggtcacc agtcgcagcc cccacgggcc aataatgcag
    22921 cctgctatca ggtagccaag taccgatccc agaccgaggc gtactgcaat gggtacaatc
    22981 agcgccgccg aaccgagata aatcagcgcc tgaatcaagg tatgactatc catgatgtgc
    23041 ctcctgccat tccagcagac gttgcttata gtgacgcgcc tgcccttcga gggtttcgtc
    23101 gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc cgcagtagat
    23161 tgccgtcgcc tgtagcggct gcgacagcac atcaaacccc ggatgcgtgc caatctcaaa
    23221 atggctttcc ccaccgccgg tcgtcaccgc ccacagtaaa tgtttgccat gcagcgccgt
    23281 gccgccgtga ccgtaagccc agccgtgcga gaagacttta tcgatccaaa gtttgaggag
    23341 cggaggaatg ctgtaccact gcatcgggtg ctgccagacg atcagatcgg cacgagacag
    23401 cgcctcctgt tcggcggcaa tgtcgatatt gaagtcagga tagagttgat aaagagagcg
    23461 aatttcgacg ccttccagcg tccttgcctg ttcaagcatc cgtttattcg catgggaatg
    23521 atgcggatat ggatgcgcat aaattataag aatcataggt agcccgcgtg attacgcctt
    23581 tgttttccca caaagtgtag tcatatattc acgcagctta tagtcaataa tattgattca
    23641 gaaaagagag aaatctgaac aaattacccc attctctgcc cgcggatcgt catattgctg
    23701 gtctgcgcca gtgacatccc tcgagtctcc ggggcaaacg ctacggaaat caacaagcca
    23761 aatagcgaga tacccgcccc cattagcatc gtgttactga taccgtaatt attgataaag
    23821 atcggcagtg cccaggtcga tacaatagtg ccaatacggc ttaaggacat aatcacgccc
    23881 acggcagagg cgcggatatc cgtcgggaag agttcattag gataaagcca ctgcaaatta
    23941 cccggcccac cggagaaaaa ggcatacacc gcaaacgcca ttaccaccag ccagatcccc
    24001 atatccggga tcagccccaa aaccgccagc gccagcgtca tcatggcaaa gctgccaatc
    24061 aacaatggac gccgtccggc agtgtttagc cacagcatcg gcggaataca gccgagcata
    24121 aagaacaggc taatcaccac gttccccagc gccgcgtttt tgccaacccc caatcccaac
    24181 aaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca ttgggatcac ctggcaggtc
    24241 cagatggcgg caacaaacag aacaaaagga aaatggcggc gattaaacag atcgcgaaaa
    24301 cgggtttgct gcggctgctc ttcctcgaaa gccaccggtt cgccaaacag ttttatcatc
    24361 atctcttcgc actcttttac tcgccctttg cgtaacaacc agcggggaga ttcaggcagt
    24421 tcgaatcgac caatcaaaat caacaaacag ggaatcgctg cgctacccag catccagcgc
    24481 cagccgcctt ccacatcata aagccaatag ccgaccaaat cggcgcaggt cgcgccgaca
    24541 taccacatcg cggcaataaa gctgatggaa aatgcccgct gacgggtact ggagaactcg
    24601 gtgatcattg aggtggcgat gggataatcc gcaccgataa caatgccgat aagtacccgc
    24661 atcaccaaca gttcgacggg ggatgagaca aacatcgtcg ccaccgatat cacgccgatg
    24721 gcgatgatat caatgaggaa cattttgcgc cgtccgactt tatcggagat atagccaaac
    24781 agcgatgtgc caacgaacag cccggcgagc gttcccgcgc ccagcaagcc aatccagtca
    24841 gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca cgccaatcat taccagtaca
    24901 taaccatcca gaaacggacc accgcttccc cacagcaaaa tgcggcggtg aatagaggag
    24961 aatttaagat catcaaagtt tctggacggt tgcatgggtt attcctgttt ttttgcaatg
    25021 agagtcagta cggcattacg tatcaaacca acatcagccg taacggaact ccacaccaaa
    25081 ggtgccgcgc gggtattccc actgttccag cgccgacccc agccccagaa tgcgacaggt
    25141 gccgcactcc agacatccgg cgtaatcgaa gcgcacactg ccgtcatcct gcttcttgta
    25201 cagacctgcg gggcacgctt tcaccagcag ctccagggcc tgtttatcag catcggcctt
    25261 cacaacgatg tgcggatgct cttcatcgac attgaattta ttgacgccca gtttgacgtc
    25321 cacattgacg ggagaagtca taaaacggtc actcctttca tgccatcctt gatcagattg
    25381 atgaagccca ctttcttgcc gtggcggagg attttcttgc gcatcagttc cggcgcactg
    25441 ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca gttccgggta gccgctaaac
    25501 atgcgtgggt tatcaagaaa cgccggtagt ttctggtaca tgcgcatatc gcgcagcggg
    25561 ccactctcaa gatgctgacg atattccgcg agtttttgct tactgaaatc gtcgcttttc
    25621 atcgctgaaa gcacagtttt tgctgctgct tccccggcgg caatcgccag atccataccg
    25681 cgaatggtaa aaccgaggtt catacacatt ccggcggcat caccagcaat caatacgccg
    25741 tcaccgacca gctccggcag catgttgatg cccgcttccg gcactacgtg agcggaatat
    25801 tccaccagct tgccgcccgc gatcagcggt gcaacggccg gatgttgttt gaaatcttcc
    25861 agcatttgcg gcaccgattt ttttgcgtca tgcagatgat gcaaaccaca aaccagcccc
    25921 agcgacaggg tattttcatt ggtataaagg aagccacctc ccatcaggcc atcggtgggt
    25981 gatcccgcaa acaggcaagc cgcgccctga ttaccctgca actgaaaacg gtcttcaata
    26041 accgacttcg gtaactcgat cagttccttc acgccaactg ccacatccgt cggtttaacg
    26101 cgttttgcca tccccagctt ttcggcaagg atggaattca ccccatcagc aaggatcacc
    26161 gttttcgctt caatcacatc gccatcggct tctacaccga cgactttgcc atcgcgctgt
    26221 acgaggttat cgacgcggat cccggtaatt aactgcgcgc ccgcttcttc ggcctgctcc
    26281 atcagccagg catcaaattt actgcgtaaa acggagtaag aacgctggga tggcgaggtt
    26341 tcgtcgccat tgcagtagtc catagtcatc gctgactttt ccgtcataaa cgcaagtttt
    26401 tcatgggtga tcaggcgttc tacgggggcg gagtcagcga aaccaggaat aatgtgttcc
    26461 agactgtggg catagagacg cccgccggtg acgttcttgg cacctgcgga attgcctcgc
    26521 tcgataacta acacttgcgc accttcgcgg gcgagcacca gtgcggcaac cgaaccggca
    26581 agccctgcac cgacgatgat ggcgtcaaag atatcttcga acataagagc tccctgtcag
    26641 cggcagggcc aaaatgcagc cctgccagag tggatcaacg cgctaaagct gcggtcagtg
    26701 ccggaaggat cttcacggcg tcgccaacaa tgccgtaatc cgcgtactgg aagatcggcg
    26761 catttttatc tttgttgatg gcgaaaatgg tttgcgacgc attagcgcca accatgtgct
    26821 ggatctgccc ggagatcccc accgccaggt acagttcagg tttcagcatc aggttggaga
    26881 taccgacata gcgttcgtgc tccatccatt tttcgttttc cgccaccgga cgagaacagg
    26941 ccaactccgc acctatcgcc ttgcaaagct gttctgccag cgcaatgttc tctttgctgc
    27001 caataccgcg accaacgctg accaccaggc gggctttgtc gagatcgacg ctgttgctct
    27061 gacgcgcctg ggttgccgtg cgggtaatcg ccacagccgg agcctgccac tccacggtgt
    27121 gcgtttcgcc agtgcgtgac gcgtctggct gagccacatc gaacgtgccg ctgctgatgg
    27181 tcagaaccgc atacggcgtg gcaatgcgtt cttcgccaat cgccagaccg ccgtaaacca
    27241 tgtgtttcac tgtcgcttta ccgtcctgca cgctaacagc gctggcatcg ttagacaccg
    27301 ccgcgttaag gcggtaaccc agtttcgccg ccagtaattt gccgcgacgg gtgtttggca
    27361 acagcaccag accgtctgcg ccgtgctggc gaatagtgtc agccatgaca ccggcgtaat
    27421 cttcgatcat ccgctcgtcc ggtttgccgc ttaatttcca gacatgatta gcgccgagct
    27481 ggattgcctg tgcgccgtcg gcatcattga ggacgaacgt gttgatttga ttagctaaag
    27541 cctgcgcacc gttcatcagt tccggcagac gagaaggggt atcgctgaat acccagactt
    27601 gagaaaacgt gttcatagca tcccctgtaa ttaaatgact ttgcgaagat tttcagcaaa
    27661 tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg cgctgacgct cgcgctgttt
    27721 cggcgcggca acctgttgtt ctgaccaggc tgcctctgcg ttaaaaccga tatccgccgc
    27781 cgaccatacc tggactggct ttttcgccgc gccgagaatg gctttcatcg aaggaatttg
    27841 tggggagttg atatcagtgg aaacagcaac aaccgcaggc agcggaatgc ttaaggtttc
    27901 tgtttcatct tccagttcgc gctcaacggt gagggtatct gccgtcaggg agataatttt
    27961 gctgacgccg ttaactgccg gaatattaag gatttcgcct actagcaaac caacctgctg
    28021 ggcataaagg tcggaagaac catcgccaca gaggatcaaa tcaaagcctg ctttctgagc
    28081 ggcagcagcc agtacgctcg ccgtttgttg cggcagtgcc tgctcgaact ggtcatcaat
    28141 caccacaatc agttcatccg gaccgcgcga tagcacatct ttacgccctt tggcgttggt
    28201 cagggcttta ccgcccacac ttaaggccgt cacctgcgcc tctactgcct gttgctttag
    28261 ctggcaagcc gcttcaatag cgttgagatc gtattggctt attttggcat cggctttgct
    28321 gaagtctaac gaaccatcag cattattgac cgcaatatcc tgttcatcag gcacgcactt
    28381 atagcaagta ataatcttca ttgcatctcc agaaatcatg aaggaaacgt cgttcatatc
    28441 ggtggtgtta attacagata tcacgccctt taatttggaa ataaaacttt aatcaccaat
    28501 attgaaaatg ccacacgcag ataaatccaa ctttcaatat tgttaagctc ctcaccaata
    28561 ttgaaaacat ggcgtagaaa aaagaaattt tcaatattgt tttatggatc accaatattg
    28621 aaagtcagat gctatttttt tactcacaac agaacataac aaactgatta ataatcaaaa
    28681 ttaacgaaaa aatgccctct attcacccga aacaaaaatg tgataacaat cacagaatgc
    28741 agcttattga atacccatta tgagttagcc attaacgcgt ccacgaggtt aataataatt
    28801 atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt cgttttcaca
    28861 gtggaattaa ctcatgaaga atgaaaagag aaaaacggga atagaaccga aggttttctt
    28921 tccgccgtta ataatcgtcg gcatactttg ttggcttaca gtcagagatc tggatgcggc
    28981 gaatgtcgtt attaatgctg tattcagtta cgtcaccaat gtatggggat gggcatttga
    29041 atggtatatg gtggtgatgc ttttcggctg gttctggctg gtgtttggcc cgtatgccaa
    29101 aaagcgttta ggtaacgaac caccagaatt tagtaccgcc agttggatct ttatgatgtt
    29161 cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg attgagatct actactacat
    29221 ctccaccccg ccgtttggct tagaaccgaa ctcaacaggc gcgaaagagt tggggctggc
    29281 ttacagcttg ttccactggg gacctctgcc gtgggccact tacagcttcc tttcagtcgc
    29341 cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt cgccccagct ccaccctggt
    29401 gccgctggta ggtgaaaaac acgccaaagg gttgttcggc actatcgtcg acaacttcta
    29461 tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc cttgccacgc cgctggtgac
    29521 cgagtgtatg caatggttgt ttggcattcc gcataccctg caactggacg ctatcatcat
    29581 tacctgctgg attatcctca acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg
    29641 tatcgccagt gacgtgcgta gttacctgag cttcctgatg ctgggttggg tgttcatcgt
    29701 cagcggtgcc agcttcatca tgaactactt caccgattcg gtggggatgt tactgatgta
    29761 tctgccgcgc atgttgttct ataccgatcc catcgctaaa ggcggctttc cgcagggctg
    29821 gaccgtgttc tactgggcat ggtgggtgat ttacgctatc cagatgagca tcttcctcgc
    29881 ccgcatctcc cgtggtcgta ccgtgcgtga actgtgcttc ggcatggtgc tggggctgac
    29941 agcgtcaacc tggatcctgt ggactgtact cggtagtaac actctgctgt tgatagataa
    30001 aaacatcatc aacattccaa atctgatcga acagtacggt gtggcgcgcg ccatcatcga
    30061 aacctgggcc gctctgccac tcagcaccgc caccatgtgg ggcttcttca tcctctgctt
    30121 tattgccacc gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt ccacttgccg
    30181 cgaagtgcgt gacggcgaag aacccccgct gctagtgcgc attggttggt cagttctggt
    30241 tggcattatc ggcattgttc tgctggcgct cggcggcctg aaaccgattc aaaccgccat
    30301 tatcgccgga ggatgcccgc tgttcttcgt caacatcatg gtgacgctct cctttattaa
    30361 agacgcgaaa cagaactgga aagattaatt aaccacccaa atatcaagag gttgaaagat
    30421 ggattttaat ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg aactgatggc
    30481 cagcgaaaac tgggaggcct attttgccga gtgcgaccgt gacagcgtct acccggaacg
    30541 ttttgtcaaa gccctggcgg atatgggtat cgacagtctg ctgatcccgg aagaacacgg
    30601 tggtctggac gcggggtttg ttactctcgc cgccgtgtgg atggaactgg gtcgtctggg
    30661 agcgccaact tacgtgctgt atcagttacc gggcgggttt aacaccttcc tgcgtgaagg
    30721 tacgcaagag cagatcgaca agatcatggc tttccgcggc accggtaagc agatgtggaa
    30781 ctcagcgatt accgaaccgg gcgcaggctc cgacgtgggt agcctgaaaa cgacttatac
    30841 ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt tttattacca gtagcgccta
    30901 caccccgtac atcgtggtga tggcgcgcga cggggcttct ccggacaaac ctgtctacac
    30961 cgaatggttt gttgatatga gcaaaccggg catcaaagtg accaaacttg aaaagctcgg
    31021 tctgcgtatg gatagctgct gtgaaatcac ttttgacgac gtggaactgg acgagaaaga
    31081 catgttcggt cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg accatgaacg
    31141 tttcctggta gccctgacca actacggtac ggcgatgtgc gcctttgaag atgcggcgcg
    31201 ctacgccaac cagcgcgtgc agtttggcga ggctattggt cgtttccagt tgattcagga
    31261 aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc
    31321 gtggaaagca gacaacggca ccatcacctc tggcgatgca gcgatgtgca aatacttctg
    31381 cgccaatgcg gcatttgaag ttgtggatag cgcaatgcag gtgctgggcg gtgtcgggat
    31441 tgcgggcaac caccgcatca gccgcttctg gcgtgacctg cgtgtagacc gcgtctccgg
    31501 gggatctgac gaaatgcaga tcctgacgct gggtcgcgcg gtgctgaagc aataccgcta
    31561 agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc aggccgacgg taggccggat
    31621 aaggcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat
    31681 ggatcatcta cccatgccga aattcggacc gctggccgga ttgcgcgttg tcttctccgg
    31741 tatcgaaatc gccggaccgt ttgccggaca aatgttcgca gaatggggcg cggaagttat
    31801 ctggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct
    31861 ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag ggcgcgaagc
    31921 gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt
    31981 tgcccgtcgt ggcattaccg atgaagtcct gtggcagcac aacccaaaac tggttatcgc
    32041 tcatctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa
    32101 cactatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat
    32161 gcctgccttc ccgtataccg ccgattactt ttctggcctg actgccacca cggcggcgct
    32221 ggcagcactg cataaagtgc gtgaaaccgg caaaggcgaa agcatcgaca tcgccatgta
    32281 tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat
    32341 gtgcccgcgc atgaccaaag gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg
    32401 cgccgacggc tacatcgtga tggaactggt gggcattact caaattgcag agtgctttaa
    32461 agatattggc ctcgcacacc tgctcggtac gccggaaatc ccggaaggca ctcagcttat
    32521 ccaccgtatc gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc
    32581 ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa
    32641 agtactgacc gtgccggaac tggaaagcaa tccacagtat gtggctcgcg aatcaatcac
    32701 tcagtggcaa acgatggatg gtcgcacctg caaagggccg aatatcatgc cgaaattcaa
    32761 aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat
    32821 tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc
    32881 caaagttgag gactaaacgc gtggcgtcag tcaacttgcc tgactagcgc ccgcccaata
    32941 acaaacgaac aaaatggata gaggtgcaat ggatatcatt ggcggacaac atctacgtca
    33001 aatgtgggac gatctggcgg acgtttacgg tcataaaacg gcgctgattt gtgaatccag
    33061 cagcggagtc gttaaccggt atagttatct tgagttaaat caggagatta accgcacggc
    33121 aaacctgttt tatacgctgg ggattcgcaa aggcgacaag gttgctctac atctcgacaa
    33181 ctgcccggaa tttatctttt gctggttcgg gttggcaaaa attggcgcga ttatggtgcc
    33241 gattaacgcc cgcctgttgc gcgaagaaag cacgtggatc ctgcaaaata gccaggcgtg
    33301 cctgctggtg accagtgcgc aattttatcc tatgtatcaa cagattcagc aggaagatgc
    33361 ctctcagttg cggcacattt gcctgacaga tatggtactt cccgctgatg atggcgtgag
    33421 ttcgtttact caactgaaaa atcaacaacc tgccaccttg tgctatgcac cgccgctatc
    33481 gactgacgat acggcggaaa ttctcttcac ctccggcacc acctcccgac cgaaaggggt
    33541 ggtgataact cattacaacc tgcgcttcgc tggatattac tccgcctggc agtgtgcact
    33601 gcgtgacgat gacgtctacc tgacggtaat gcctgcgttt catatcgatt gccagtgtac
    33661 tgcggcgatg gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga aatacagcgc
    33721 ccgcgccttc tggggacagg tgcagaagta ccgcgccacc attaccgaat gtattccgat
    33781 gatgattcgt acgttgatgg tgcagccgct ttcagcgaac gatcagcaac accgcctgcg
    33841 ggaagtgatg ttttatctca acctgtcgga gcaggaaaaa gacgcgtttt gtgaacgctt
    33901 cagcgttcgc ttgctgacgt cttatgggat gacggaaacc attgtgggca ttatcggcga
    33961 tcgccctggc gataaacggc gctggccgtc gattggtcgg gcgggctttt gctacgaagc
    34021 ggagatccgc gacgatcaca atcgtcccct cccggctggt gagctcggtg aaatctgtat
    34081 taaaggcgta ccagggaaaa ccatcttcaa agagtatttt ctcaacccga aagccactgc
    34141 gaaagtgctg gaagccgatg gctggctaca taccggcgat accggatacc gcgacgaaga
    34201 gggctttttt tatttcgtcg atcgccgctg caatatgatc aaacgcggtg gcgagaatgt
    34261 ctcctgcgtg gagctcgaaa atatcatcgc cacccatcca aaaattcagg atatcgtggt
    34321 tgtgggtatt aaagattcga ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga
    34381 aggtgaaaca ttgagcgaag aggaattctt ctgcttctgt gaacagaata tggcgaaatt
    34441 taaagtgccc tcttatctgg agatcagaaa agatctgcca cgtaattgct cggggaaaat
    34501 aattagaaag aatctgaaat aaatgaaacg gcagggaact accctgtcag ctaataatca
    34561 cgctctaaaa caatatgcat tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg
    34621 gagaaaagga atgagtgaat cattacatct gacccgcaat ggatcaattc tggaaattac
    34681 ccttgatcgt ccaaaagcga atgctattga tgcaaaaacc agctttgaaa tgggcgaagt
    34741 atttctaaat ttccgtgacg atccgcaatt acgtgtcgcc attattaccg gtgccggaga
    34801 gaaattcttt tccgcgggct gggatttaaa agcggcagca gaaggcgaag caccggatgc
    34861 tgactttggc ccgggaggtt ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt
    34921 catcgccgct gtgaacggct atgcctttgg cggcggcttt gaactggcgc tggcggcaga
    34981 ttttattgtt tgtgccgata acgccagctt cgccctaccg gaagccaaac tgggcatcgt
    35041 tcctgacagc ggcggcgtgc tgcgtctgcc gaagatcctg ccgcctgcca tcgtcaatga
    35101 aatggtgatg accggcagac gaatgggcgc agaagaggcg ctgcgttggg gggtagtcaa
    35161 ccgcgtggtt agccaggcgg aactgatgga taacgcccgc gaactggcgc agcagctggt
    35221 taacagcgct ccgctggcga ttgcggcgct gaaagagatc taccgcacca ccagtgaaat
    35281 gccggtagaa gaagcgtatc gctatattcg cagcggcgtg ttgaaacact atccgtcggt
    35341 tctgcattcg gaggatgcca ttgaagggcc gctggcgttt gccgagaagc gcgatccagt
    35401 gtggaaagga cgttaacaac cgtgagctat tacgcctttg aggggttaat tccggttgtt
    35461 cacccgacgg cgtttgtcca tcccagtgca gtattgattg gcgatgtgat tgtgggagcc
    35521 ggtgtctaca tcggcccact cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa
    35581 gcgggagcca atattcagga tggctgcatt atgcatggct actgcgacac tgacaccatc
    35641 gttggggaaa acggtcatat cgggcacgga gcgatcctgc atggttgtgt gattggtcgc
    35701 gatgcactgg tcgggatgaa cagcgtgatt atggatggcg cggtcattgg cgaagagagc
    35761 attgttgccg ctatgagctt tgtcaaagcg ggctttcgcg gcgagaaacg ccagttgttg
    35821 atgggtacgc cagcccgcgc tgtacgcagc gtgagtgacg acgagttaca ctggaaacgg
    35881 ttgaatacca aagagtatca ggatcttgtc gggcgctgtc atgcagcgtt acatgaaacg
    35941 cagccactga ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg
    36001 ccgaaacggt aacatcgtcg gatgcggcat aaacgcctta tccgacctgg ctgtaaagca
    36061 accgtaggcc ggataagatg cgccagcatc gcatccagcg accaggtcgc acggtatcaa
    36121 cgaaatgaca ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt
    36181 taagttccgt gagcggaacg gcaggaatac gcggcgtctt tcttacgccg actaatttat
    36241 cccggctgtt attgcgcagg cgcgcgtaaa tttgctcatc gatatcaacc acttttacca
    36301 gtcgctggca ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc
    36361 ttatttctgt gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa
    36421 tatcgaaatg aatagagatc tctcttgcta tcacccaccg gttttcagcc atcatccatt
    36481 cggcgattaa caaatagagt ggtttttcaa cacatccttc acacatacga actccagttt
    36541 cacaaattaa aagactggct atgactcgtt ttgcagcaat taaacaaggc catattaaaa
    36601 ttgtggatat atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt
    36661 atgtcatcaa aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaacggtcct
    36721 catcagagaa ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccatgac
    36781 acgctattat ttgatctgcg cgtgcatttc ctgcaccgaa attacttttt cggtcgcatc
    36841 ggcattcagc gccatcgcgg tggcaaagcc gccgttcagg gtggtgtcgt aatgcacttt
    36901 atattgcagc gcactgcgac ggatcacgcg ggagtcttca atcgcacgac gacctgaggt
    36961 ggtgttgatg atgtaggtat attcgccatt cttaatacgg tcctgaatgt gcggacggcc
    37021 ttcatgcacc ttgttcacca gacgcggatt gatacccgct tcgcccagca caatcgccgt
    37081 gccgtgagtc gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac
    37141 gcgttcttta tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga
    37201 gttgctgccc agctgcgctt tggcaaacgc ttcagcgaag gtgcggccca cgcccatgac
    37261 ttccccggta gagcgcattt ctggccctaa cagcgggtca acgcccggga atttattgaa
    37321 cggcagcacc acttctttca ccgagtagta cggcgggata acttctttgg ttacgccctg
    37381 ctcagccagc gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt
    37441 ggctttggag acaaacggaa cggtacgcgc cgcacgcggg ttaacttcaa tcaggtagac
    37501 ttcgttgttt ttcaccgcaa actgcacgtt catcaggccg cgcacctgca attcgaaggc
    37561 cagtttctgc acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtaggctgg
    37621 cagcgaacat gcggagtcac cggagtgcac gcccgcctgc tcgatgtgct ccatgatgcc
    37681 gccaatcagc accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc
    37741 aaggaagtgg tccagcaaca ctggcgcatc gttagatacg ctgaccgctg tctggaagta
    37801 gcgacgcagg tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga
    37861 cggacgcacc accagcgggt agccaatctc tttcgctttc tcaaccgcca tttcgatggt
    37921 ggtaacggtg gcgttcgccg gttgtttcag tttcagacgc tcaaccgcat gctggaagcg
    37981 ttcacggtct tccgcacggt cgatggcatc cgggctggta ccgataaccg gtacgccagc
    38041 agcttccagc gcgcgcgcca gtttcagcgg ggtctgaccg ccgtactgga cgataacgcc
    38101 tttcggcttc tcaatacgca caatttccag cacgtcttcc agagttaccg gctcgaagta
    38161 gaggcggtcg gaagtgtcgt aatcagtgga gacggtttcc gggttacagt taaccataat
    38221 ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt acacagcagt agtcgaattc
    38281 gataccctga ccgatacggt tcgggccgcc gccgaggacc atgattttgt cacggtcaga
    38341 tgggttggat tcgcactctt cttcataagt ggagtacatg taagcggtgt cggtggcgaa
    38401 ctctgccgca caagtatcca cgcgcttgta aaccgggtgc agatcatact ggtcgcgcaa
    38461 cttgcggatt tccgcttcgc gcacgcccgc cagtttagcc agacgcgcat cggcaaagcc
    38521 tttgcgtttc aactggcaca ggaattcagc gtgcaggcca gtgatgccca cttctgcgac
    38581 tttctcttcc agacgcacca gctcttcaat ctgtaccagg aaccagcggt caatgttggt
    38641 caaattaaat acaccgtcta cagacaggcc cgcgcggaag gcatcagcga tataccagat
    38701 acgctctgcg ccagcatctt tcagttcgcg gcggatttta gtcagcgctt ccgggtcgtc
    38761 gagactcact ttcggatcga agccagttgc gcccacttcc aggccgcgca gcgctttttg
    38821 tagggattcc tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt tcatctgagt
    38881 ggtcagacgg tcgttagcac cggcgaattt ctcgaagttg aagcgaggaa ttttagtaac
    38941 cacatagtcg atggacggct cgaaggaggc cggagtacgc ccaccggtga tgtcgttcat
    39001 cagctcgtcg agggtgtaac ccaccgccag tttcgccgcc actttggcaa tcgggaaacc
    39061 agtggctttc gacgccagcg cagaggagcg agacacgcgt gggttcattt cgataacaat
    39121 caggcgaccg tttttcgggt tcaccgcaaa ctggacgttg gaaccaccgg tttcaacgcc
    39181 gatttcacgc agtaccgcca tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag
    39241 cgtttgggct ggcgcgacag tgatggagtc accggtgtgg atgcccatcg catcaaagtt
    39301 ttcgatagag cagacgatga tgcagttgtc gtttttatca cgcaccactt ccatctcgta
    39361 ctctttccag ccgatcagcg actcatcaat cagcagctct ttagtcggag aaagatccag
    39421 accgcgggcg caaatttctt caaattcttc acggttataa gcgataccgc caccgctacc
    39481 gcccatggta aaggatgggc gaataatgca cgggaagcca acttcagcgg caaccgccag
    39541 cgcttcttcc attgaatgag cgataccgga acgcgcagtt tccagaccga ttttcttcat
    39601 cgctacgtcg aaacggcggc ggtcttcggc tttatcaatc gcatcggcag tggcaccaat
    39661 catggtgacg ccgaactctt ccagcacgcc ctgacgctca agctccagcg cacagttcag
    39721 cgccgtctgc ccccccatgg ttggcagcac cgcgtccggg cgctcttttt caataatctt
    39781 gcgtaccact tcccagtgaa tcggctcgat gtaggttgca tcagccattt ccgggtcggt
    39841 catgatagtc gccgggttgg agttcacgag aatgacgcgg taaccctctt cacgcagggc
    39901 tttacacgct tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc
    39961 cgcacccaga atcaggatac tttttatatc tgtacgtttt ggcatggctc ttttactcct
    40021 gattacttag cggttttacg gtactgctca attaacgcga taaagtggtc aaacaacggc
    40081 gcggcgtcgt gtggaccagg gctggcttcc gggtgtccct ggaagctgaa cgccggttta
    40141 tcggtacgat gaatgccctg taacgtaccg tcgaacaggg atttatgtgt gacacgcagg
    40201 tttgcaggta atgttgcttc atccaccgca aaaccgtggt tctgggcggt gatcatcacc
    40261 acatttttct caacatcttt aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg
    40321 atggtcttcg caccgctcgc cagcgccagc agctgatgac cgaggcagat gccgaatacc
    40381 ggaatatcag tttcgaggaa tttctggata gcggtaatgg cgtaatcgca cggggccggg
    40441 tcgccaggac cgttggagag gaagatgccg tctggattca ttttcagcac atcttccgca
    40501 gaagtttgcg ccggaacgat ggtcaggcga cagcctctat ccaccagcat acgcaggatg
    40561 ttgcacttgg caccgaaatc gtaggccacg acgtggtacg gcagctcgtc ttcttttttc
    40621 gcttctggca ggccgccggt cagtgtccag ctcccttgtg tccagctata gggttctgcg
    40681 gtggttactt cttttgccag atccatgccg ttcagacctg ggaacgcgcg ggctttttct
    40741 aacgccagcg ccgcatccgg gttgtcaccc gcaataatgc agccattctg agcacctttc
    40801 tcacgcagta aacgcgtcag cttacgggta tcgatatcgg caatcgccac gatgttatgg
    40861 cgcttcaggt aagaagagag gtcttcggta ttacggaagt tgctggcaat cagcggcagg
    40921 tcgcgaatca ccagaccttg ggcatgaacc tgagaagatt cttcatcggc gtcattagtg
    40981 ccgacattgc caatatgggg ataagtaaga gtaacgattt gacgagaata ggaaggatca
    41041 gtgaggattt cttgataacc ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa
    41101 cctgttgccc ctatggcccg accgtgaaac tgggttccgt cttccagaac caatagcgct
    41161 gacttaatca aaacaccctc cagagaatat tcactcactt tatttgcata ttaattcaca
    41221 atgatgacgt aaatcaatgc aaatctgctt accgacgaat ttctggcaaa cggcggcatt
    41281 ctggagatat gggagctaaa agtcaactta atgg
//
LOCUS       DAZFRG010000010        40976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFRG010000010 DAZFRG010000000
VERSION     DAZFRG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.010"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..511
                     /gene="yccU"
                     /locus_tag="ACHKUC_000377"
     CDS             98..511
                     /gene="yccU"
                     /locus_tag="ACHKUC_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO6616704.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(556..873)
                     /gene="hspQ"
                     /locus_tag="ACHKUC_000378"
     CDS             complement(556..873)
                     /gene="hspQ"
                     /locus_tag="ACHKUC_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO6616705.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(931..2121)
                     /gene="rlmI"
                     /locus_tag="ACHKUC_000379"
     CDS             complement(931..2121)
                     /gene="rlmI"
                     /locus_tag="ACHKUC_000379"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO6616706.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2216..2494
                     /gene="yccX"
                     /locus_tag="ACHKUC_000380"
     CDS             2216..2494
                     /gene="yccX"
                     /locus_tag="ACHKUC_000380"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO6616707.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2491..2820)
                     /gene="tusE"
                     /locus_tag="ACHKUC_000381"
     CDS             complement(2491..2820)
                     /gene="tusE"
                     /locus_tag="ACHKUC_000381"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO6616708.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2911..3570)
                     /gene="yccA"
                     /locus_tag="ACHKUC_000382"
     CDS             complement(2911..3570)
                     /gene="yccA"
                     /locus_tag="ACHKUC_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO6616709.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFMLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3777..3864)
                     /locus_tag="ACHKUC_000383"
     tRNA            complement(3777..3864)
                     /locus_tag="ACHKUC_000383"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3828..3830),aa:Ser,seq:tga)
     gene            4291..5409
                     /gene="hyaA"
                     /locus_tag="ACHKUC_000384"
     CDS             4291..5409
                     /gene="hyaA"
                     /locus_tag="ACHKUC_000384"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO6616710.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGELGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     IMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            5406..7199
                     /gene="hyaB"
                     /locus_tag="ACHKUC_000385"
     CDS             5406..7199
                     /gene="hyaB"
                     /locus_tag="ACHKUC_000385"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO6616711.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFSEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            7218..7925
                     /gene="hyaC"
                     /locus_tag="ACHKUC_000386"
     CDS             7218..7925
                     /gene="hyaC"
                     /locus_tag="ACHKUC_000386"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO6616712.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYGIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            7922..8509
                     /gene="hyaD"
                     /locus_tag="ACHKUC_000387"
     CDS             7922..8509
                     /gene="hyaD"
                     /locus_tag="ACHKUC_000387"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO6616713.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAYIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
                     QPTEELRCLNYDCLSMENYEGVRLRQYRMRLEKQG"
     gene            8506..8904
                     /gene="hyaE"
                     /locus_tag="ACHKUC_000388"
     CDS             8506..8904
                     /gene="hyaE"
                     /locus_tag="ACHKUC_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO6616714.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            8901..9758
                     /gene="hyaF"
                     /locus_tag="ACHKUC_000389"
     CDS             8901..9758
                     /gene="hyaF"
                     /locus_tag="ACHKUC_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO6616715.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            9892..11436
                     /gene="appC"
                     /locus_tag="ACHKUC_000390"
     CDS             9892..11436
                     /gene="appC"
                     /locus_tag="ACHKUC_000390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO6616716.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            11448..12584
                     /gene="appB"
                     /locus_tag="ACHKUC_000391"
     CDS             11448..12584
                     /gene="appB"
                     /locus_tag="ACHKUC_000391"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6616717.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            12597..12689
                     /gene="cbdX"
                     /locus_tag="ACHKUC_000392"
     CDS             12597..12689
                     /gene="cbdX"
                     /locus_tag="ACHKUC_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO6616718.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            12769..14079
                     /gene="appA"
                     /locus_tag="ACHKUC_000393"
     CDS             12769..14079
                     /gene="appA"
                     /locus_tag="ACHKUC_000393"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO6616719.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIANEARIPACALHQDK"
     gene            complement(14067..14279)
                     /gene="cspH"
                     /locus_tag="ACHKUC_000394"
     CDS             complement(14067..14279)
                     /gene="cspH"
                     /locus_tag="ACHKUC_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO6616720.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            14565..14777
                     /gene="cspG"
                     /locus_tag="ACHKUC_000395"
     CDS             14565..14777
                     /gene="cspG"
                     /locus_tag="ACHKUC_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO6616721.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            14788..14976
                     /gene="ymcF"
                     /locus_tag="ACHKUC_000396"
     CDS             14788..14976
                     /gene="ymcF"
                     /locus_tag="ACHKUC_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6616722.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            14951..15181
                     /gene="ymcE"
                     /locus_tag="ACHKUC_000397"
     CDS             14951..15181
                     /gene="ymcE"
                     /locus_tag="ACHKUC_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO6616723.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVRIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            15171..15344
                     /gene="gnsA"
                     /locus_tag="ACHKUC_000398"
     CDS             15171..15344
                     /gene="gnsA"
                     /locus_tag="ACHKUC_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO6616724.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(15393..16466)
                     /gene="yccM"
                     /locus_tag="ACHKUC_000399"
     CDS             complement(15393..16466)
                     /gene="yccM"
                     /locus_tag="ACHKUC_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6616725.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHARCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAIYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(16538..19281)
                     /gene="torS"
                     /locus_tag="ACHKUC_000400"
                     /pseudo
     CDS             complement(16538..19281)
                     /gene="torS"
                     /locus_tag="ACHKUC_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            19364..20392
                     /gene="torT"
                     /locus_tag="ACHKUC_000401"
     CDS             19364..20392
                     /gene="torT"
                     /locus_tag="ACHKUC_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO6616726.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(20365..21057)
                     /gene="torR"
                     /locus_tag="ACHKUC_000402"
     CDS             complement(20365..21057)
                     /gene="torR"
                     /locus_tag="ACHKUC_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO6616727.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            21187..22359
                     /gene="torC"
                     /locus_tag="ACHKUC_000403"
     CDS             21187..22359
                     /gene="torC"
                     /locus_tag="ACHKUC_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO6616728.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            22359..24905
                     /gene="torA"
                     /locus_tag="ACHKUC_000404"
     CDS             22359..24905
                     /gene="torA"
                     /locus_tag="ACHKUC_000404"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO6616729.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDISQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYGENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            24902..25501
                     /gene="torD"
                     /locus_tag="ACHKUC_000405"
     CDS             24902..25501
                     /gene="torD"
                     /locus_tag="ACHKUC_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO6616730.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTATVNGLENSIATLTVRDDARLELAADFCGLFLMTDNQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LRQWLPEFVARCHQYDRFGFYAALSQLLQVLVECDNQKG"
     gene            complement(25858..26163)
                     /gene="cbpM"
                     /locus_tag="ACHKUC_000406"
     CDS             complement(25858..26163)
                     /gene="cbpM"
                     /locus_tag="ACHKUC_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO6616731.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(26163..27083)
                     /gene="cbpA"
                     /locus_tag="ACHKUC_000407"
     CDS             complement(26163..27083)
                     /gene="cbpA"
                     /locus_tag="ACHKUC_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO6616732.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            27236..27337
                     /locus_tag="ACHKUC_000408"
                     /pseudo
     CDS             27236..27337
                     /locus_tag="ACHKUC_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27618..28859
                     /gene="agp"
                     /locus_tag="ACHKUC_000409"
     CDS             27618..28859
                     /gene="agp"
                     /locus_tag="ACHKUC_000409"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO6616733.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(28897..29124)
                     /gene="yccJ"
                     /locus_tag="ACHKUC_000410"
     CDS             complement(28897..29124)
                     /gene="yccJ"
                     /locus_tag="ACHKUC_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO6616734.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(29145..29741)
                     /gene="wrbA"
                     /locus_tag="ACHKUC_000411"
     CDS             complement(29145..29741)
                     /gene="wrbA"
                     /locus_tag="ACHKUC_000411"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6616735.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            30114..30287
                     /gene="ymdF"
                     /locus_tag="ACHKUC_000412"
     CDS             30114..30287
                     /gene="ymdF"
                     /locus_tag="ACHKUC_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6616736.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(30370..31698)
                     /gene="rutG"
                     /locus_tag="ACHKUC_000413"
     CDS             complement(30370..31698)
                     /gene="rutG"
                     /locus_tag="ACHKUC_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO6616737.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(31719..32213)
                     /gene="rutF"
                     /locus_tag="ACHKUC_000414"
     CDS             complement(31719..32213)
                     /gene="rutF"
                     /locus_tag="ACHKUC_000414"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO6616738.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQESLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(32224..32814)
                     /gene="rutE"
                     /locus_tag="ACHKUC_000415"
     CDS             complement(32224..32814)
                     /gene="rutE"
                     /locus_tag="ACHKUC_000415"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO6616739.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(32824..33624)
                     /gene="rutD"
                     /locus_tag="ACHKUC_000416"
     CDS             complement(32824..33624)
                     /gene="rutD"
                     /locus_tag="ACHKUC_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO6616740.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(33632..34018)
                     /gene="rutC"
                     /locus_tag="ACHKUC_000417"
     CDS             complement(33632..34018)
                     /gene="rutC"
                     /locus_tag="ACHKUC_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO6616741.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(34030..34722)
                     /gene="rutB"
                     /locus_tag="ACHKUC_000418"
     CDS             complement(34030..34722)
                     /gene="rutB"
                     /locus_tag="ACHKUC_000418"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO6616742.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(34722..35813)
                     /gene="rutA"
                     /locus_tag="ACHKUC_000419"
     CDS             complement(34722..35813)
                     /gene="rutA"
                     /locus_tag="ACHKUC_000419"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO6616743.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDNAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            36101..36739
                     /gene="rutR"
                     /locus_tag="ACHKUC_000420"
     CDS             36101..36739
                     /gene="rutR"
                     /locus_tag="ACHKUC_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO6616744.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(36779..40741)
                     /gene="putA"
                     /locus_tag="ACHKUC_000421"
     CDS             complement(36779..40741)
                     /gene="putA"
                     /locus_tag="ACHKUC_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6616745.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDERVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAQVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            40796..>40976
                     /locus_tag="ACHKUC_000422"
     CDS             40796..>40976
                     /locus_tag="ACHKUC_000422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616746.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECN"
BASE COUNT         9740 a        10712 c        10961 g         9563 t
ORIGIN      
        1 gacatatttc ccgcttttgc tctcattcat tcgtattagc tgcatggttg gcatgtcggc
       61 tttgtcgtac actttgttaa acagtctgga ggaaataatg aaagaaaccg atattgcagg
      121 cattttaacg tccactcaca ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc
      181 cagctatcgc gtgatgaaat atctactcga ccaggggtat cacgtcattc cggtttcgcc
      241 aaaagttgct ggcaaaacac tgctgggcca gcaggggtat ggcacgctgg cagaagttcc
      301 ggaaaaagtc gatatggtcg atgttttccg gaattcagaa gcggcgtggg gcgtagcgca
      361 ggaagcgatc gccatcggcg cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca
      421 agcggcagta ctggctcggg atgccggatt aaatgtagtg atggatcgct gcccggctat
      481 tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg
      541 ggatttgttc agggattagt tacgcagacg cggggcctgg agttgtttgc ggatggtctg
      601 cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg
      661 ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca ccacgtgata
      721 ccacggagcg gcgcgaagct cgtcattcac cgccagctca tcaggcgacg gttcagagag
      781 cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac
      841 ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt
      901 tgatataagg gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag
      961 atatagccct tccgggtagg tagcgatcac cggatgatcg gctgcctgac ggaactgctc
     1021 tataaattgt acatcacgac cggcatcaat tgcggcatcc gcgatgattt tctgaaataa
     1081 atcgctggtc atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcaa
     1141 ctgaatcgcc agcatgttga tgtctttata accacgacac gcgcccatca actggctttt
     1201 attctcaaca aacttcggcg ggtccatcac gataacatca aatttttcac cgcgatcgcg
     1261 ataagtacgc agcaatttaa agacatcatc acggacaaac tctgccttgc tcagatccag
     1321 tttgttcagc tcaacgttct gccgtgcaat atccagcgct tcctgggagg tatcaacgct
     1381 gacaacctgg ctgcaaccgc ccatcaatgc cgatacggcg aaaccaccgg tataggagaa
     1441 acagttaagt acgcgtttat tttcgacgta gcggcgggta gccaggcggc tgtcacgctg
     1501 gtccaggtag tagcccgttt tgtgtccgtg ctgaatatcc accagcagtt tcatgccgtg
     1561 ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg atggggccct gagtcagctc
     1621 catcccttct tttttacgta ccgcgacgtc gctgcgatcg taaatagcac attccgggta
     1681 cagcgtttgc agggcgctaa ttaatgccgc gcgctgatac tctgcgccag cactcagcag
     1741 ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc
     1801 tccggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagccagt cacgccattt
     1861 ttgtgcttgc tgcaaacggc gggtaaaaaa agcaatgtcg atagactctg acgggtcaaa
     1921 cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt
     1981 tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt ccatgcgggc
     2041 aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc
     2101 taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt
     2161 gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag cagcaatgtc
     2221 gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgttggat ttcgctatac
     2281 cacgcagtac gaagcgaaaa aactgggatt agccgggtac gccaaaaatc ttgacgatgg
     2341 cagcgtggaa gtggttgcct gcggtgaaga agggcaggtg gaaaaattaa tgcagtggtt
     2401 aaagagtggc ggcccgcgtt ctgcgcgtgt ggaacgggtg ctcagcgagc cgcatcatcc
     2461 ctcgggggaa ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc
     2521 agcaattttg gtggcttgct tagccggacc tttcggaaac agtcggtata aatagcggct
     2581 gtttcctttt tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg
     2641 agaagtattg aattccagat agaaatcacg cacaaaacgc accacttccc agtgttctgg
     2701 cgacagcgta atcccttcgt tctctgcaat caccactgcc agcggctcac tccactggct
     2761 gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat
     2821 aattattact accttaatca gactggcggc agtgtaacaa aaaacaaagc cccgcataaa
     2881 gcgaggctcg gtaagtaacc ttgggcgaga ttaatcgcgg ctggcgaagc ccagaatgct
     2941 cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaaccgtgg cacgaatata
     3001 gttcgtttca ccgccatgaa tgatgttgct ggtttcaaac aaaatagcgc cagaggagat
     3061 cagaatgaag accgcgctga tcgccagatg cagagcaggc agttgcagga agatattagc
     3121 aaccatgcca atcagcacca ccacaatacc cgccatcagc ataccgccaa ggaacgacat
     3181 atctttgcgg gtggtcagca catacgcaga gcagcagaag aacaccaacg ccgttccgcc
     3241 cagtgccata gcgattacgt cacccattcc ggcagacagg taggtgttca gaattggtcc
     3301 gaggatatat cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt
     3361 cgccgtttta taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc
     3421 tggagatggc agcatcagca ccgtactggc agttgcggta atcgccgaaa aggccagcgt
     3481 caggctcagc ataaaatagg tattacgcag caccttatgg gtgctaagca gtgatgtacg
     3541 gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa
     3601 ttaagcagca taatgataat gcgtaagggc acccagaagt tttacccatc tttacgcatt
     3661 tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata acgccctgag
     3721 aatttcgaca ggcaaaagaa aaaggggtta gcagttagct aaccccttat cttatttggc
     3781 ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca agaccggtgc
     3841 cttcaaccgc tcggccacac ttccggaatg acgcgcacta taaacatccc gatgcggcgt
     3901 gtaaacccct aatttgtttg tttgcctgaa aaacagtcaa aagtgcattg atagcgtgaa
     3961 ataacagcag attgatcatt tcatcaccat gaattccttc tcttttactc gtttagcaac
     4021 cggctaaaca tccccactgc ccggcaaaaa gaaaaatagg tccattttta tcgctaaaag
     4081 ataaatccac acagtttgta ttgttttgtg caaaagtttc actacgcttt attaacaata
     4141 ctttctggcg acgtgcgcca gtgcagaagg atgagctttc gttttcagca tctcacgtga
     4201 agcgatggtt tgccttgcta cagggacgtc gcttgccgac cataagcgcc cggtgtcctg
     4261 ccggtgtcgc aaggagaaga gacgtgcgat atgaataacg aggaaacatt ttaccaggcc
     4321 atgcggcgtc agggcgttac ccggcgcagc tttctcaaat attgtagtct ggctgccacg
     4381 tcgctgggat taggcgcggg aatggcacca aagattgcct gggcgctgga gaacaaaccg
     4441 cgcattccgg tggtatggat ccacggtctg gaatgcacct gctgtaccga atcttttatc
     4501 cgctccgctc acccgctggc aaaggacgtc atcctttccc tgatttccct cgattacgac
     4561 gatactttga tggctgccgc cggaacccag gcggaagaag tctttgaaga catcatcacg
     4621 caatacaatg gcaaatatat cctcgccgtt gaaggtaatc cgccgctggg cgagctgggg
     4681 atgttctgta tcagcagcgg tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga
     4741 gccagtgcga ttatcgcctg gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga
     4801 cccaatccga cgcaggcaac gcctatcgac aaagtcatca ccgacaaacc tattatcaaa
     4861 gtacctggct gcccaccgat cccggatgtg atgagcgcca tcattactta catggtgacc
     4921 tttgatcgct tgccagatgt cgacataatg ggccgtccgt tgatgttcta tggtcagcga
     4981 atccacgata aatgctatcg ccgcgctcac ttcgacgccg gagagttcgt gcagagttgg
     5041 gatgatgacg ctgcccgcaa aggttactgc ctgtacaaaa tgggctgcaa agggcctacc
     5101 acctataacg cctgttcctc cacacgctgg aacgatggcg tttccttccc gatccagtca
     5161 ggtcacggct gcctgggttg tgcggaaaat ggtttctggg atcgcggttc gttctacagc
     5221 cgcgtggtcg atattccaca aatgggtacc cattccaccg ccgacaccgt cggtttaacc
     5281 gcgcttggcg tggtggcagc ggctgttggt gtgcacgcag tcgccagcgc cgttgaccag
     5341 cgcagacgtc ataaccagca acctacagaa accgaacatc agccaggcaa tgaggataaa
     5401 caggcatgag cactcagtac gaaactcagg gatacaccat caacaatgcc ggacgccgcc
     5461 tggtggtcga cccgattacg cgcattgaag gccacatgcg ctgcgaagtg aatattaacg
     5521 atcagaatgt gatcaccaat gccgtctcct gcggcaccat gtttcgcggg ctggagatca
     5581 tcctgcaagg gcgtgacccg cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct
     5641 gtactggcgt acacgccctg gcttcggttt acgccatcga agatgccatc ggtattaaag
     5701 tgccggataa cgccaatatc atccgcaaca ttatgctggc aacgctctgg tgccacgatc
     5761 atctggtgca cttctatcag cttgccggga tggactggat cgatgtgtta gatgcgctga
     5821 aagccgaccc gcggaaaacc tccgaactgg cgcaaagcct ctcctcatgg ccgaaatcat
     5881 cccctggcta tttcttcgac gtacaaaacc gcctgaagaa attcgttgaa ggcgggcagt
     5941 tggggatctt ccgcaatggc tactgggggc acccgcagta caaactgccg ccagaagcca
     6001 acctgatggg ctttgcccac tatctcgaag ctctcgattt ccagcgtgaa attgtcaaaa
     6061 tccacgcggt ctttggtggt aaaaacccgc atccaaactg gattgtcggc gggatgcctt
     6121 gcgccattaa cattgacgaa agcggcgcag tcggggcggt caatatggaa cgcctgaacc
     6181 tggtgcagtc gatcattacc cgtacggcgg atttcattaa caacgtgatg atccccgacg
     6241 ccttagccat cggccagttc aacaaaccgt ggagcgaaat cggcacgggt ctttctgata
     6301 aatgtgtcct cagctacggc gcattcccgg atattgccaa cgactttagt gagaaaagtc
     6361 tgctgatgcc tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg ccagtggatt
     6421 tggttgatcc gcagcaggtg caggagtttg tcgaccacgc ctggtatcgt tatcccaacg
     6481 atcaggtggg gcgtcatccg ttcgatggca ttaccgaccc gtggtacaac cccggcgatg
     6541 tcaaaggcag cgataccaac attcagcagc tgaatgaaca ggaacgctac tcgtggatca
     6601 aagcgccacg ctggcgcggt aacgcgatgg aagtggggcc gctggcacgc acgttaatcg
     6661 cttatcacaa aggcgatgct gcgaccgttg agtcggtcga tcgtatgatg tcggcgttga
     6721 aactgccgct ttccggcatc cagtcaacgt tgggccgcat tttgtgtcgc gcgcacgaag
     6781 cgcagtgggc cgcaggtaag ttgcagtatt tcttcgataa actgatgacc aacctgaaaa
     6841 acggcaatct tgccaccgct tccacggaaa aatgggaacc agcaacctgg ccgacagagt
     6901 gccgtggtgt cggttttacc gaagcgccgc gcggggcgtt aggccactgg gctgccattc
     6961 gcgatggcaa gattgatctc taccagtgcg tggtgccgac cacctggaac gccagcccgc
     7021 gcgatcccaa agggcagatt ggcgcttatg aagcggcgct gatgaacacc aaaatggcga
     7081 tccccgagca accgctggag atcctgcgta ctctgcacag ctttgacccg tgcctcgcct
     7141 gttcaacaca cgtgctgggc gacgacggta gcgagcttat ctccgtgcag gtgcgttaac
     7201 agcgaaggag aatcatcatg caacagaaaa gcgacaacgt tgtcagtcac tatgtctttg
     7261 aagcacccgt gcggatatgg cactggctga cagtgttatg catggcggtg ttgatggtca
     7321 ccggttactt tatcggcaag ccgctacctt ccgtcagcgg cgaggcgacg tatctgttct
     7381 atatgggcta catcaggtta attcacttca gcgccgggat gatttttacc gtggtcttgc
     7441 tgatgcggat ctactgggct tttgttggca atcgttactc ccgcgagctg tttatcgtgc
     7501 cggtatggcg taaaagctgg tggcagggcg tgtggtatgg aatccgctgg tatctgtttc
     7561 tggcaaaacg tccgagtgcc gatataggcc ataaccccat cgcccaggcg gcgatgttcg
     7621 gctatttcct gatgtcagtt tttatgatca tcaccggctt tgcgctgtac agcgaacaca
     7681 gccagtacgc tatttttgcg ccgttccgtt atgtggtgga atttttctac tggacgggtg
     7741 gcaactcaat ggacattcac agctggcatc ggctggggat gtggctgatt ggcgcgtttg
     7801 tgatcggtca tgtctacatg gcgctgcgtg aagacatcat gtccgacgac acggtgatct
     7861 ccactatggt caacggctac cgtagccaca aatttggcaa aataagtaac aaggagcgtt
     7921 catgagcgag caacgcgtgg tggtaatggg gctgggtaac ctgctgtggg ccgatgaagg
     7981 cttcggcgtg cgggtggcgg aacggctgta tgcccattac cactggcccg agtatgtgga
     8041 gattgtcgat ggcggcaccc aaggactgaa cttgctgggg tatgtcgaaa gcgccagtca
     8101 tctgttgatt ctcgatgcca ttgattacgg gctggaaccc ggaacgctac gaacgtatgc
     8161 cggagaacgc attccagctt atctcagcgc gaagaaaatg agcctgcacc agaacagttt
     8221 ctccgaagtg ctggcgctgg cagatatccg cggacatctg ccagcatata ttgccctcgt
     8281 cggtctgcaa cccgcgatgc tcgacgacta cggcggcagc ctgagcgaac tggcgcggga
     8341 gcaactgccc gctgcggaac aggcggcgct ggtacagctg gcggcctggg gaattgtacc
     8401 gcaaccgact gaggagttgc gctgtctcaa ttatgactgt ctgtcgatgg aaaattacga
     8461 aggcgttcgc ttgcgtcagt acaggatgag gctggagaaa cagggatgag caacgacaca
     8521 ccatttaatg cgttgtggca acgaatgctg gcacgtggct ggacgccggt cagtgaatgc
     8581 cgtcttgacg actggcttac gcaagcgcca gacggcgtgg tgttattaag tagtgacccg
     8641 aaacgcacgc cagaagtcag cgataacccg gtaatgattg gcgaattact gcgcgagttt
     8701 cccgactata catggcaggt agcgattgct gatcttgagc agagcgaagc catcggcgat
     8761 cgctttggcg tctttcgctt tcccgccaca ttagtgttta ccggcggaaa ctatcgcggc
     8821 gtgctgaatg gtattcaccc gtgggcggaa ctgataaacc tgatgcgcgg gcttgtcgaa
     8881 ccgcaacagg agcgtgcttc atgagcgaaa cttttttcca tctgctgggg ccaggaacgc
     8941 aaccgaacga tgacagtttc agcatgaatc cactgccgat cacctgtcgg gtgaatggtg
     9001 aaccgagtat ggcggccctg gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa
     9061 acgagttaca acatcaacta agcgaacgcc aaccgccgtt gggcgaggtg ctggcagtcg
     9121 atctgttaaa tctcaacgcc gacgatcgtc actttatcaa tacgcttctc ggggaagggg
     9181 aagtgtcagt gcgcattcag caggctgacg acagtgaaag tgaaattcag gaggcgatct
     9241 tctgcggatt atggcgggtg cgcagacgtc acggcgaaca gttgctggaa gacaaactgg
     9301 aggctggctg cgcaccgctg gcgttgtggc aggcggcaac gcaaaacgtc ttgccaacag
     9361 actcgttgct accgccgccc attgatggcc tgatgaatgg cctaccgttg gcccatgaat
     9421 tactggcaca tgtacgtaac cccgacgcgc agccgcacag cattaatctg acgcaattac
     9481 ccatcagcga agctgaccgg ctttttctct cacgtctctg tgggccggga aatattcaga
     9541 ttcgtaccat tggctatggc gagagctata tcaacgccac ggggttacgc catgtctggc
     9601 atttacgctg tacggacacc ttaaaaggcc cgttactgga aagttatgaa atctgcccaa
     9661 taccggaagt ggtgctggca gcgccagaag atttggtcga ctctgcgcag cggcttagcg
     9721 aggtatgcca gtggctggcg gaaggtgcac cgacgtaaaa agacggtaag tatcgctttc
     9781 aggcttatga atatcgcaat cggcgaatac ctctggtcgt agagtttcag gataaagagg
     9841 gagatctacc attaacgggt tatttttctc tcttcgccta caggagtgcg catgtgggat
     9901 gtcattgatt tatcgcgctg gcagtttgct ctgaccgcgc tgtatcactt tttatttgta
     9961 ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta cgtggtcacc
    10021 ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg tatcaacttt
    10081 gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa ctggtcattc
    10141 tattccaact atgtgggcga tatttttggc gcaccgctgg cgatggaagc attaatggcc
    10201 ttcttcctcg aatccacctt tgtcgggctg ttcttcttcg gctggcagcg gctgaataaa
    10261 taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc tgcgttgtgg
    10321 atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga tatcgacacc
    10381 ctgcgtatgg agatgaccag tttcagcgag ctggtcttta acccggtcag ccaggtgaaa
    10441 tttgtgcaca ccgtaatggc gggatacgtg accggggcta tgtttattat ggcgatcagc
    10501 gcctggtatt tactgcgcgg acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt
    10561 tccgtcttcg gtactctggc gattatcggt acgctgcaac tcggagacag ttctgcgtat
    10621 gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg gcaaacggaa
    10681 cctgcacctg caccgttcca tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc
    10741 tttgctatca aaattcccgc gctgctagga atcctcgcca ctcattcatt agataaaccc
    10801 gtgccgggtc tgaagaattt gatggctgaa acctacccac gcttgcaacg cggacgtatg
    10861 gcgtggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt gttgcaggca
    10921 ttccgggagc tggaaggcga cctgggttac ggcatgttgc tctcccgcta tgcgccggat
    10981 atgaatcatg tcacagccgc acagtaccag gcggcgatgc gtggcgcgat acctcaggtt
    11041 gcgccggtat tctggagttt ccgcatcatg gtgggctgtg gttccctgct gctactggtg
    11101 atgctgattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg
    11161 aaaatggcgc tctggagttt gccgctgccg tggattgcga ttgaagccgg atggtttatg
    11221 accgagtttg gtcgtcagcc gtgggcgata caagacatct taccgacata ctccgcgcac
    11281 tccgccttaa ccacaggaca actggctttc tcactgatca tgatcgtagg gctttacacc
    11341 ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg gccgagcgcg
    11401 atgcagagtg aacaaccgac gcagcaacag gggtaaagga gaaaatcatg tttgattatg
    11461 aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtggtc tttatgatct
    11521 ccgacggatt tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt aatgatgatg
    11581 aacgtcggat agtgataaac agcgttggcg cgcactggga aggcaatcag gtctggttga
    11641 tcctcgcagg tggggcatta tttgccgcct ggcccagagt gtatgcagcg gcgttttccg
    11701 gcttttatgt ggcgatgatt ctggtgctgt gctcgctgtt cttccgcccg ctggcctttg
    11761 attatcgcgg aaaaatcgcc gatgcacgct ggcgtaaaat gtgggatgcc ggactggtca
    11821 tcggcagtct ggtgccgccg gtagtcttcg gtattgcatt cggcaacttg ctgctcggcg
    11881 tgccgtttgc tttcacaccg caattacgcg tggaatatct cggcagcttc tggcaactgc
    11941 tgacgccatt ccctttattg tgtggattgc tcagtcttgg gatggtgatt ttgcaaggtg
    12001 gcgtctggtt acaactgaaa actgttggtg tgattcatct gcgttcacag ctggcgacca
    12061 aacgcgccgc actactggtg atgctgtgct ttttgctggc gggttactgg ctgtgggttg
    12121 gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat ccgctaatga
    12181 aactggtggc agtgctacct ggtgcctgga tgaataattt cgtcgaatcg cccgttttgt
    12241 ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg atttatcgtg
    12301 gtcgcccggg ttggggattt ttgatggcgt cattgatgca atttggcgtg attttcacgg
    12361 caggcatcac gctgttcccc tttgtcatgc cgtcaagcgt gagtccgatc tccagcctga
    12421 cgttgtggga cagcacttcc agtcagctga cgctgagcat tatgttggta atcgtgctga
    12481 tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg tgggggcgca
    12541 tgacgacaga aactctccgc cgtaacgaaa acgagctgta ctaaggagca ggaacaatgt
    12601 ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc cttgtgttgg
    12661 tatggctgga cccgcgtctg aaaagttaac gaacgtaagc ctgatgtggc gcattagcat
    12721 ctcatcaggc aatcaataat gtcagatatg aaaagcggaa acatatcgat gaaagcgatc
    12781 ttaatcccat ttttatctct tctgattccg ttaaccccgc aatctgcatt cgctcagagt
    12841 gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt gcgtgcacca
    12901 accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac ctggccggta
    12961 aaactgggtt ggctgacacc gcgcggtggt gagctgatcg cctatctcgg acattaccaa
    13021 cgccagcgtc tggtggctga cggattgctg gcgaaaaagg gctgcccgca gcctggtcag
    13081 gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc cttcgccgcc
    13141 gggctggcac ctgactgtgc aataaccgta catacccagg cagatacctc cagtcccgat
    13201 ccgttattta atcccctaaa aactggcgtt tgccaactgg ataatgcgaa cgtgactgac
    13261 gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg gcaaacggcg
    13321 tttcgcgaac tggaacgagt gcttaatttt ccgcaatcaa acttgtgcct taaccgtgag
    13381 aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa ggtgagcgcc
    13441 gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga gatatttctc
    13501 ctgcaacagg cacaaggaat gccggagccg gggtggggaa ggatcactga ttcacaccag
    13561 tggaacacct tgctaagttt gcataacgcg caattttatt tactacaacg cacgccagag
    13621 gttgcccgca gtcgcgccac cccgttattg gatttgatca tggcagcgtt gacgccccat
    13681 ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtgctgtt tattgccgga
    13741 cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac gcttccaggt
    13801 cagccggata acacaccgcc aggtggtgaa ctggtgtttg aacgctggcg tcggctaagc
    13861 gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca gatgcgtgat
    13921 aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct ggcaggatgt
    13981 gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat cgcgaatgaa
    14041 gcacgcatac cagcgtgcgc tctccatcaa gacaaataaa catttgccgc tgttggtcca
    14101 cgcaggccat ttacgcggca aaattccacg cgtaatcctg gtataagcac ttctgcgtcg
    14161 cggggagtga atgcggaaat atggacctgg acttctttac gaccgtcgga ggggataatg
    14221 aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt acgggacaaa
    14281 caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat aaagccagcg
    14341 atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta tcatgcgtta
    14401 atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa gcagttctca
    14461 ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta gtttctgtat
    14521 taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct aataaaatga
    14581 ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact cctgatgatg
    14641 gcagcaaaga cgttttcgtc catttcactg ccatccagag caatgaattc cgcacgctga
    14701 acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggtccc gcggcagcga
    14761 acgttgttac gctctaaggt tgccgttatt actcaacatc tccatttccg ctgtccatgt
    14821 tgccatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa ccctttgggt
    14881 gcaaagtgca ttttttgtaa atcgacgatg atcacctttg ataacgtcgc gctgcaaata
    14941 cgcactgacc atgcgccgct ggatttcaca aaataatatc aggctccctc gtggagcctt
    15001 ttttatatct gccttatttt tcttcaatgc tgtacgtata gtaagcgata acctgttgat
    15061 tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg taccgggaac
    15121 caacacctta ctggcgtgtt gctgtctttt aagaccagaa gaggttaaca gtgaatattg
    15181 aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa aaaatcgccg
    15241 agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca cgagaaaaaa
    15301 tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt gaacatcacc
    15361 ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcattgg cgattgcgga
    15421 attaagcgtt ggtacaattc ctccggcacc gggctttgcc atactcccgc atagattgcg
    15481 taaccaatcg ccgcaaacat aatccccata accagtagcg tcattaacca tccagacaac
    15541 gcaaaggctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc
    15601 ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac ctgaattaat
    15661 ttatcgaccg ggatccgtga tgggcaactt tttgcgcatt tgccacaatc gatacaactt
    15721 tcggcattgc gacgaatctt aaacggcgat aatagcgaaa ccacgcccat cagcgcgcca
    15781 tatgggcaaa gataacgaca cctggcatgg cgaataaaca ggctggcaat cagcaaaacg
    15841 atcatgctga ttaatgtcgc ggtccccata tgacgaaaga aatcgagcat tttaacgtcc
    15901 atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcggg catcaataac
    15961 gcgatataga gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg aatatccagc
    16021 cagcggggaa ggacaaattg ccgaccaaac agtttgttac cgagatcgcc gattaattca
    16081 gaaagcgtac caaccgggca taaccatgaa caaaaggcct ttttgagtaa tagactgatg
    16141 acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa ctgacctgtt
    16201 gcaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc
    16261 ggtctggcga caaatgtcgt actacctgcc gtttcgtaat agcgcaccca ataccagaac
    16321 gtgatggcaa tataaatatt aattgccaac agtaataatt gcgtcgcttt acgccaggtc
    16381 gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc
    16441 cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc atacaaaatg
    16501 ccactattct agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct
    16561 tattaaccat gcttccagag cggcaacact gcgtgtaatt tcttcgtgtg gaaggggggc
    16621 agataatggc tgctgctcca gttgtgcgca ttgctggctg gcgctacgca ttcccagact
    16681 cgagcaactg ctttttagct gatgtgcggc acgctttatt ttctcgttat cctgactggc
    16741 gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt tgtttaaata atatcagcca
    16801 ttcgtggatc ttctccgccc ccattaaatg agcatcttca ttgagttgcg atacatccag
    16861 cggttgatcg ttattgactt gcagttggag atagtgcgcc agtaattgac cgagcacttc
    16921 acgcggcacc ggtttaggga taatcccgcg aaatagcgaa ctggtacgct gtcgcagtgt
    16981 ttcgtcaatg acatgggcgc taaagccaat caaaaccagc gacggatatt gctgtgccag
    17041 ttgtcgggca agcgtaatgc cgtcgatatc cggcagatca aaatccacca gtgcggcagc
    17101 aaacggttca ctattttgca gtgtttctaa agcctgcacg gcattgccaa cagcaacaac
    17161 ctgcgcacca ctgatgttca gcatctcaac ggtaattcgc tgggttagcg ggttatcttc
    17221 aattaacagc aaacgtaaac cgtccagacg caccgcctga ttgactgttt ttggcacggg
    17281 tgcagtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaacct ccggcgtgct
    17341 ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatag tcagccccag
    17401 cccggtgccg ccgcgtttgc cgctaacctg aacaaatggc tggaagattt ctgccagttt
    17461 cgcgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgtt cgccatcagt
    17521 gcgactacgc aggataatgt gcccttcgtc ggtgaaacgc agggcgttgc tcaacaggtt
    17581 ggtgatgacc tgacgaatac gccgcggatc gcccattaac gcagtcggta catcgtcggc
    17641 aatttctgtt gccaggcgaa tcgggcgacc tttcaccctt ccgctcatta attgcagggt
    17701 actttccagc agcgggcgcg gttcaaaggg ttcatcgctg accgaaacat tcttgccacc
    17761 tgcttcgata gccgaataat cgagaatatc gttgaggatg gtcagcaacg attcaccaga
    17821 gtcggtaatc gcccgcaaat catcgcgctg ggcgttaagt gcggggttat ctgccagcag
    17881 ttgcgcggtg ccgagaatac cgtacagcgg tgtgcggatc tcatggctca tcgccgccag
    17941 aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc ggtgttctat
    18001 caccagttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct gttcgcggtg
    18061 gcgattcagt gcatgaacac tgctgcgaaa cgcatccatc agccgcccga tggtatccag
    18121 ttcccgtacg ccagcggttt ccgggaaagg ggagtcgata tcaccatcca gcaagcgttg
    18181 cagcgcctgc gtttgttctg caagagggcg cgtgactgag cgataaacca cgcgccagag
    18241 gatcagaatc agtgcgcaaa gagaaactaa tcccagcaat aacaggctgt attgcccgcg
    18301 tgcactggct ttttccagat gtgccagccc gtgctgatta cgcagctcaa tggtgtcgac
    18361 cagctgactg acttcgctac taaactgcgc gaattgggcg atgttatttt gggcgagagt
    18421 ttgcaggtga ttgctgattt cactgtcctg ctgatacagc gccagcaaat cgctatattg
    18481 gctaacggta gttaacgttg ttgctacctg agtacgaaca cccggatctt caatgcgtat
    18541 ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg ttggcgcatt
    18601 tttctggatc tgttccagcc ccagattcat caccatttgc tgcacccgta gggcgctaag
    18661 gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc gatcgagtgc
    18721 actttctgcg gcctgacgct gatgttgttc gatcaaatcg taaatcccgg cctgggtcgc
    18781 tccggcggag gttgccgcat tattcgcctg accttgcgcc aggcgtgcga tctcatcggc
    18841 ggcggcgact atctgctgac tgagttgctg ttgttgctgg cgcagttgca aacgctgtcc
    18901 caccagttcc ccttgctgac gtaacgaacg ggagatctcc tgctcctgtt gttcaatagc
    18961 ggtggtgtca aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct tcaggctttg
    19021 tgcggtgagc attcgcccct gcgcctgcca catcttttcg ttatcggcac tggtcaggtt
    19081 ctgcgcggcg aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca tattcatggt
    19141 aggaatcaat gcctgagtgt tgtctttttc caccaggctg ataaagcgca ggttgtacca
    19201 tcccaccagg gtactggtca gggttaacag cgccatcagg gcaaagccca tccagagtct
    19261 tcgggtcagg gttaaattca cggtcggtgc actttaggtg aaaaaggttg agtcgcaaag
    19321 cagaatgcat ctagcataaa gccttattat tgatgaggct atcatgcgcg tactgctatt
    19381 tttacttctt tcccttttca tgttatcggc attttcggct gataacctgt tgcgctggca
    19441 tgatgcgcag cattacacgg tgcaagcctc tacgccgctt aaagccaaac gcgcatggaa
    19501 actgtgcgcg ctttatccca gcctgaaaga ttcatactgg ttatcgttga actatggtat
    19561 gcaggaggct gctcgccgct acggtgtgga tttaaaagtg ctggaggcag gcggctacag
    19621 ccagttggct acccagcaag cacaaatcga ccaatgtaaa cagtggggcg cagaggccat
    19681 tttgctcggt agtagcacga cgtcatttcc cgatctgcaa aagcaggtag caaatctgcc
    19741 ggtgatcgaa ctggtgaatg ctattgatgc tccccaggtg aaaagccgcg ttggtgtgcc
    19801 ttggtttcag atgggctatc aaccagggcg atatctggtg caatgggcgc acggtaaacc
    19861 actgaacgtg ctgttgatgc ccggccccga taacgccggg ggcagtaagg agatggtaga
    19921 gggttttcgc gcagccattg ccggaagccc ggtacgtatt gttgatattg cgctcggtga
    19981 taacgatatt gaaatccagc gtaacctgtt gcaggagatg ctggaacgcc atccagaaat
    20041 cgacgtcgtt gccggaacgg ccattgcggc agaggcggca atgggggaag ggcgcaacct
    20101 gaaaacgccg cttaccgtgg tgtcgtttta tctttcacat caggtgtatc gcgggttgaa
    20161 gcggggaaga gtgattatgg cagccagcga tcaaatggtc tggcaggggg aactggcggt
    20221 tgagcaggct atcaggcaat tacaggggca atcggtgtct gataatgtca gcccaccgat
    20281 tttagtcatg acgccgaaaa atgccgaccg cgaacatatc cgtcgctcgc tgtcaccagg
    20341 gggatttcgt ccggtctatt tttatcagca cacatcagcg gctaagaaat aaccttcgcc
    20401 atgttgtgtc accagtaaat ctgcactgag tttatggcgt aaacgacgaa ttaacacatc
    20461 gacggtgcgc aggtcagggt tttccacccg acgcgcagag agcatacgca gcagacgttc
    20521 acggctgaga atttcgccgg gattggtcac aaacgccacc agcatttcat actctgcgcg
    20581 ggtcagttta atcggctctc catcccgctc cagcgtatgg cgcgacacat tcaggcaata
    20641 accggcaaag cgatagcagt tgtcctgagt gtgcggttgc gcctgtcgcg cgaggtcgat
    20701 tcgccagagc aaatttttca ctcgtaccac cagttcgcgc agctccagcg gtttggtgac
    20761 gtaatcgtct gcgcccattt ccagcccaac aatacggtca atccgatcgc tgcgtccggt
    20821 aaccagaata atccccaccg ttgagcgttc tcgcagggcg cgggttaaca tcaggccatt
    20881 ttcatcgggc aaattgatat ccagcagaat caaatccacc ggctgattct gcataatttc
    20941 ccgcagcccg gcaccgctcg ccgtaacgga aacggtatac ccctcctgag tgaagtaaga
    21001 ttgtaatcgc gcctgggtaa ccggctcatc ttcaacaata acaatgtgat gtggcatcag
    21061 agggttttac tcattctgtt catatctgtt catattctgc cgtaagccgt tcatcctgac
    21121 cagtgccgct gttcatattt gctcattaag atcgcttcac taaaccataa ttttacaggg
    21181 gctattatgc ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg
    21241 gcgctggtcg ctactgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg
    21301 atcaaagtca ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat
    21361 gaagaatata aacagtcggt gcatttccag aacgcctccg gcgtgcgagc tgaatgccat
    21421 gactgtcata tcccgccgga tattccaggc atggtgaagc gcaaactgga agcgagcaac
    21481 gacatttacc agacctttat tgcccactcc attgatacac ctgagaaatt tgaagccaaa
    21541 cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgc
    21601 cgctcctgcc ataactacga tgcgatggat catgcgaagc agcatcctga agcggcgcgt
    21661 cagatgaagg tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc
    21721 caccagttac cggatatgag tagtggcttc cgtaagcagt tcgatgagct gcgcgccagt
    21781 gctaatgata gtggtgacac gctgtactcc attgatatta agccgattta tgcggcgaaa
    21841 ggcgataaag aagcgtctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt
    21901 gacggcgact ggctgcaaat tgaaatcact ggctggacgg aaagtgcagg acgtcagcgt
    21961 gtactcaccc aattcccagg taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag
    22021 cagcaggtga aaacactgga gaaaaccacc gttgccgaca ccaataccga gtggagcaag
    22081 ttgcaggcca ctgcgtggat gaagaaaggc gacatggtga acgatatcaa accgatctgg
    22141 gcttatgcgg attcgctgta caacggcacc tgtaaccagt gccacggcgc accggaaata
    22201 tcccactttg acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagc
    22261 ctcgataaac gtgaagaacg caccttgttg aaatatctgc aaatgaatgc gtctgacacc
    22321 gcaggtaaag ctcacggcga taagaaggaa gaaaaataat gaacaataac gatctctttc
    22381 aggcatcacg tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg
    22441 ggccgtcatt gttaacgccg cgccgtgcga ctgcggcgca agcggcgact gaggctgtca
    22501 tctcgaaaga gggcattctt accgggtcgc actggggggc tatccgcgcg acggtgaagg
    22561 atggtcgctt tgtggcagca aaaccgttcg aactggataa atatccgtcg aaaatgatcg
    22621 ccggattgcc ggatcatgta cacaacgcgg cgcgtattcg ttacccgatg gtacgcgtgg
    22681 actggctgcg taagcgccat ctgagcgaca tctcccagcg cggtgataac cgttttgtgc
    22741 gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga actggaacgc gtacagaaaa
    22801 ctcacgggcc gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata
    22861 acgcttcggg gatgctggcg aaagctattg ccttgcatgg taatagcgtt ggtacgggcg
    22921 gagattactc taccggtgct gcgcaggtga tcttgccgcg cgtggttggc tcaatggaag
    22981 tgtatgaaca gcaaacctct tggccgctgg tattgcagaa cagcaaaacc attgtgctgt
    23041 ggggctccga tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt
    23101 atgaatatta cgagcagcta aaagcgaaag tcgccgccgg tgaaattgag gtcatcagca
    23161 tcgatcctgt tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg
    23221 cggttaaccc gcaaactgac gtgccgctgc aactggcgct ggcgtatacg ctgtacggtg
    23281 aaaacctgta cgacaaaaac ttcctcgcta actactgtgt aggttttgag cagttcctgc
    23341 cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct gaaaaactga
    23401 ccggcattga tgccgaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc
    23461 agattattgc tggttggtgc gtacagcgta tgcagcacgg tgaacagtgg gcgtggatga
    23521 ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc gggtggtggc ttcggttttg
    23581 gctggcacta taacggcgca ggcacgccgg ggcgtaaagg cgttattctg agtggtttct
    23641 ccggttctac gtcgattccg cctgttcacg acaacagtga ctacaaaggc tacagcagca
    23701 ccattccgat tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg atcaactgga
    23761 acggtaaatc ggtaaaactg ccgccgctga aaatgtgtat ttttgccgga actaacccgt
    23821 tccatcgcca tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg gaaacggtta
    23881 tcgccataga taaccagtgg acctcaacct gccgctttgc cgatatcgtg ctgcctgcga
    23941 ccacgcagtt tgagcgtaac gatctcgacc agtacggcaa ccactccaat cgtggcatca
    24001 tcgccatgaa acaggtcgtg ccgccgcagt tcgaggcgcg caacgacttt gatattttcc
    24061 gcgagctgtg ccgccgcttt aatcgcgaag aagcctttac cgaagggctg gacgagatgg
    24121 gctggctgaa acgcatctgg caggaaggtg tacaacaagg caaaggacgc ggcgttcatc
    24181 tgccagcgtt tgatgacttc tggaataaca aagagtacgt cgagtttgac catccgcaga
    24241 tgtttgttcg ccaccaggca ttccgcgaag atccggatct cgaaccgctg ggcacgccga
    24301 gtggcctgat tgagatctac tcaaaaacca tcgccgacat gaactacgac gattgtcagg
    24361 ggcatccgat gtggtttgag aaaatcgaac gctcccacgg tgggcctggc tcgcagaagt
    24421 atccgttgca tctgcaatct gtgcatccgg atttccgact tcactcgcag ttatgtgagt
    24481 cggaaactct gcgtcagcaa tataccgtag cgggtaaaga gccagtattt attagcccgc
    24541 aggatgccag cgcgcgcggt attcgtcacg gtgatgtggt acgcgtcttt aacgttcgtg
    24601 gtcaggtgtt ggctggagca gtggtttctg accgttatgc acccggcgtg gcgcgaattc
    24661 acgaaggggc atggtacgat ccggacaaag gcggcgagcc aggggcgctg tgcaaatacg
    24721 gtaaccccaa cgtgttgacc atcgacatcg gtacttcgca gctggcgcag gcgaccagtg
    24781 cgcacactac gctggtggaa attgagaagt acaacggaac agtggagcag gtgacggcgt
    24841 ttaacggccc cgtggagatg gtggcgcagt gcgaatatgt tcccgcgtcg caggtgaaat
    24901 catgaccacg ctgacagcac aacagattgc ctgtgtttac gcctggctgg cgcagttgtt
    24961 ctcccgtgaa ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga tggctgaatg
    25021 gttttcgttg ctgaaaagcg aacctccgct cactgcgacg gtgaatgggc tggaaaacag
    25081 tattgccact ctgacagtac gtgacgatgc ccgtctggaa ctggccgctg acttttgcgg
    25141 cctgtttctg atgaccgaca atcaagcggc gctgccgtat gcatcggcct acaaacagga
    25201 cgagcaagag attaaacgct tgttagttga ggcggggatg gaaaccagcg gcaatttcaa
    25261 cgaaccggca gatcatctgg cgatctatct cgagttgttg agtcatctgc atttttcgct
    25321 gggagagggg accgttcctg cgcgaagaat cgacggtttg cggcaaaaaa cactgacggc
    25381 gctgcggcaa tggttaccgg aatttgttgc gcgttgccat cagtatgacc gctttggttt
    25441 ttacgcagca ctaagccagt tgttgcaggt gttggtggag tgcgacaacc aaaagggata
    25501 atgccgattt gtaggcctga taagacgcgt taagcgtcgc atcaggcatt atggcgcagt
    25561 tgccggatgc ggcgagaacg ccttatccgg cctacgggaa cagtgctttt tgtgggcctg
    25621 ataagatgcg ttaagcgtcg tatcaggcat tagtgcgcag ttgccggatg cggcgagaac
    25681 gccttatccg gcctacggga acagtgcttt ttgtgggcct gataagatgc gttaagcgtc
    25741 gcatcaggca ttatggcgca gttgccggat gcggcatgaa cgccttatcc ggcctacggg
    25801 aacagtgctc tttgtaggcc tgataagacg cgttaagcgt cgcatcaggc aacaaactca
    25861 cgggtgagcc acaaaccggg aaagccgctg gcgcagcagg cggttttcct gcttcaggtg
    25921 cgcaatatca tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc
    25981 atgacgcagg cgtaccgcgc gttgcaccac aatggcggca tggtcgtcaa atacccaggt
    26041 tgtctcctgg atctcactcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc
    26101 ttcagagatg ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc
    26161 cattatgctt tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc
    26221 tgccacagcg cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca
    26281 tacagatcgc cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc
    26341 ccggcctggc tgccaggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg
    26401 actttagtac ccagcgccgc ttcccacggg ctaaccggca ccactatttc cagatcctgg
    26461 ccgacaatat caaacagcgg atgtggcgca atgtgaatca ccagccacag gtcgccattt
    26521 ggaccgccgt tttcgcccgg cgtcccctgg cctttcaggc ggatgcgttg accattgccg
    26581 acgcccgccg ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag
    26641 gcgttataaa ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtttcttcg
    26701 aggaaaaccg ccacttcgat ttcaatatcg tggccgcgtg tggcggggcg ttgacggctc
    26761 tggcgggcat gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcgttaaaa
    26821 ctctgaccgt cgccatgatg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac
    26881 atctgatcat actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcggcgacc
    26941 tctttgaagc gggcttcggc atccggttct ttgctgacat caggatggta tttgcgggcc
    27001 agtcgacgat aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg
    27061 gcgtaataat cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa
    27121 cccctgtaag gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca
    27181 gaatatgtta ttgaaatcat cgcggagggg aggttgcctc aaggtgggag gaacatattt
    27241 tatttacctc actcctgaac ttttggctca tgcttacaag ttaaacaata aggaaaggaa
    27301 tgcacaactc gactatttaa atcgttatgc aatttaatga taaagacatg tgctaaaaac
    27361 cattgctatt agtctcacat tcttttttta ttggtaatgg taacgccgct gattttcttt
    27421 atagctactg atgtaatcat tagcggcgta aacgaggccg gggctaatca ggcatagttt
    27481 gcgtcaaaac ttgcctgttt ttaaagggga atatagaaaa acaggcgttc aacgagccat
    27541 tttgcgaacc tgttcccgga aaaatgacat atttctgtca cactctttaa ttattgataa
    27601 caaaagaggt gtgaggaatg aacaaaacgc taatcgccgc aactgtggca gggatagttt
    27661 tactcgcttc aaatgctcag gcacaaaccg taccggaagg ctatcagcta cagcaagtcc
    27721 ttatgatgag ccgtcataac ttacgtgcgc cgctggcgaa caatggcagt gtgctggagc
    27781 agtcgacgcc gaataaatgg cctgaatggg acgtccccgg tgggcagctc accactaaag
    27841 ggggcgtgct cgaagtgtat atgggccatt atatgcgtga atggctggca gaacagggga
    27901 tggtgaaatc aggggaatgc ccgccaccgg acaccgttta tgcctatgcc aatagtctgc
    27961 aacgcaccgt cgcgaccgcg cagttcttta ttactggcgc attccctggg tgtgatattc
    28021 ctgttcatca ccaggaaaaa atgggcacta tggaccccac ctttaaccca gtgatcaccg
    28081 atgattccgc cgcattcagt gaacaggcgg tggcggcaat ggagaaagag ctcagcaaac
    28141 tccagcttac cgacagctac aagctactgg aacaaatcgt taactataaa gattctcctg
    28201 cgtgtaaaga gaagcaacaa tgttcgcttg tagatggcaa aaataccttt agcgcgaagt
    28261 atcaacaaga accgggcgtt tccgggccgc tgaaagtcgg caactcgctg gtagatgcgt
    28321 ttactttgca atactacgaa ggttttccga tggatcaggt ggcctgggga gaaatcaaat
    28381 ctgaccagca gtggaaggtg ttgtcgaagc tgaaaaacgg ttaccaggac agcctgttta
    28441 cctcaccgga agtggcgcgc aatgttgcga aaccgctggt cagttatatc gacaaagccc
    28501 tggtcaccga tcgcaccagt gcaccgaaaa tcacggtatt agttgggcac gactccaaca
    28561 ttgcctctct gttaacggcg ctggatttca aaccgtatca gttgcatgac cagaacgaac
    28621 gcacgccgat tggcggcaaa atcgttttcc agcgttggca tgacagcaaa gccaatcgcg
    28681 acctgatgaa aattgaatat gtgtaccaga gtgcggaaca gttacgtaat gccgatgcgt
    28741 taaccctgca ggcacctgcg cagcgcgtga cgctggaatt aagcggttgc ccgatagatg
    28801 ccaacggttt ttgcccaatg gataagtttg atagcgtatt gaatgaagcg gtgaaataac
    28861 agaaaactcc cccgcgtgaa gcgggggagt cgctggttaa acgtttttac gttcgatggt
    28921 ctgttcgccc caaaaaagcg aatctttatc ggtcttagca aaggctttga ctaacacttc
    28981 atcgctacct tcttcccaaa tcttttccgc cattttttcg tcgtacccgg cgacttcgaa
    29041 aatggcctcg gctatttccg gcgacgtatt gcgcagagat gcccattcac cgacgtgatg
    29101 agctttcgct tcttgagttg gcatgcgtat cctcctgttg aagattagcc gttaagttta
    29161 actgccagac ctgcgacata ttccccttga taacgagcaa tagacagttc ttcctggctg
    29221 ggctggcgtg aaccgtcacc gcctgcgatg gtggttgcgc cgtacggcgt accgccgcga
    29281 acctgtgaaa cgtcaaataa ttcctgcgct gcgtagccaa tggggacaat caccatgccg
    29341 tgatgcgcaa gggtcgtcca ggtggatgtg atggtttgtt cctgaccgcc gccagtaccg
    29401 gtggaactaa agacgctcgc cagttttccg tatagtgcgc cggaagccca caggccgccc
    29461 gtctggtcga ggaaggtacg catttgaccg gacatgttac caaagcgggt aggtgtacca
    29521 aaaataatgg cgtcgtaatc ggccagttct tgcggggttg caaccggtgc agtttgcgtt
    29581 ttaccgcctg ctttttcaaa taattgcggc ggcatggttt ccggtacacg cttaacgaca
    29641 acttctgcgc catccacttt gcttgcaccc tcagcaactg cgcgtgccat cgtttcaata
    29701 tgtccgtaca tggaataata aagcaccaga actttagcca tttctaacca ctcctcgtgt
    29761 tatctctatt ccgtagcgat tcgctaccac ttatttaaag ataagacgtc cttttcagag
    29821 tgcaaatttc acaaccactt atttgattta taacaacttt cacaagcacg taattttgtc
    29881 gcaaaatgac acatttttat ctcatcgcgt ttttttaatc ataagagcgg cttatggata
    29941 attattggag atgatatcta ttctcgctaa gaagctgttg caggatatta ccaaacgcgg
    30001 gtctgcccgc gtcagttcac taagcttagt cccacgtagc gaaaatatgg cagccgccat
    30061 acgccgcgtt aattctatgc aatatgatgt ctatacccag acggaggtca gtaatggcaa
    30121 accatcgagg cggttccggc aattttgcag aagaccgcga aagagcatca gaagcaggta
    30181 aaaaaggtgg acagcacagc gggggtaatt tcaaaaatga cccgcagcgc gcatcagaag
    30241 caggtaaaaa aggtggcaag agcagtcacg gtaaaagcga caactagccg ggctaatcaa
    30301 tgacgcatgc atttttgtct gtagctcgtc aaaacgccat caccgccggt taccccggcg
    30361 gttttctgtt tatggctcct gatgaacaac ttctggcggt ggaacgtcaa ccaaccttct
    30421 gcttaacaac gcattgagta aaatcgcgcc aaaggtggct gtaccgatcc ctcccaacgt
    30481 aaaaccgccc agtgtgagag caaaatcacc cgcacccagc actaaggtta ctgcgaccat
    30541 aatcaaatta ccgttctggc ttaaatccac actgttttgt acccagatcc gtgcgcctgc
    30601 gacggcaatc agcccgaaca caacaattga tgcaccacca atcaccgcag ccggaatagt
    30661 atggatcagc gcgccaaatt tcggcgaaaa gcccaacagc atggcgatga cggcagcggc
    30721 aacaaacacc agcgtcgagt agacttttgt cacggccatc acaccgatat tctcggcgta
    30781 ggtggttacg ccgctaccgc cgacagagcc agaaagcatc gtcgccagcc catcgcctac
    30841 gaatgcccgc cccatatacg ggtccatatt gcgtccggtc atcccggcga ccgccttgag
    30901 atgacctaag ttttccgcca ccagaatcac cgccacgggc gcaatcagca tcattgcctg
    30961 accattaaaa gcaggagtgg aaaaatgtgg cagaccgaac caggcagcat ggctgacgag
    31021 agtaaaatcg acggcttttc ccagccctaa aacgttggtc atcacgccat acagcagaca
    31081 ggcgacaatt aatcctacga gaatcaataa tcgctggatc atgccacggg taaatacggc
    31141 caccagccca atacatagca ccgtcattac cgccatccag ctatcaaagg ccgaagccga
    31201 tacatttttc actgcgatag gcgctaagtt caggccaatc gccatcacta ccgcacccgt
    31261 cacaaccggc ggcattagtc gttcaatcca gcgcgtaccg attttcatca ccaccaggcc
    31321 aatgacggta taaaccagcc cacaggcgat aatcccgccc agcgcgatgc tgatattcgg
    31381 attaattccc tgaccattaa agcccgtcgc ggcaatcacc acgccgacaa aagccgcgct
    31441 ggaaccgaga taactgggca cgcgcccgcc ggtaataaag aaaaacagta acgtgccgat
    31501 ccccgacatt aaaatggaaa gattgggatc cagccccatc agaattggca ttaacaccgt
    31561 cgcgccaaac atcgccaccg cgtgttgaac gcccattact gccgtttgag caaacggcaa
    31621 tcgttcatcc ggcgcgacca cgccgctctc tgtagaggtc gattttaact gccagtgagg
    31681 aaaaccgaac attgccatca gctgtctcct taaggaggtt aacaagcagg gcgcatcagc
    31741 gcgtgataac tgcgatcgaa ccacaccagc ccgtagggtg tggcgtgacg atgaatcgct
    31801 tcgatggcgc aaaacaaaat gtcgtgggtg ccgacgctca ccacctggct gatacggcag
    31861 tcaaacgaaa ccagtgcctc ttccagttgc gggcatccgg tcacaccagt ctgccagcgg
    31921 gcggcggcaa agcggtgttc catgggcgtt ttgccgccaa aaaggtttga aagcgactcc
    31981 tgcccggcgc taagtgtatt tacacacagc gttcgatttt cattgaatac cggccagacg
    32041 gacgccccac gattcaggca caccagtaat gtgggcggcg tgtcggtcac gctgcagacg
    32101 gcgctggcgg tgaacccggc gcgcccggct ggaccgtccg tagtgatgat attgaccgcc
    32161 gcacccatac aggacatcgc atcgcgaaag gtttgttgat cgacaatgtt cattgtttgc
    32221 tccttacaac agcccgcagg cttcttcaaa ggacagacgt ggcaggcgcg caaaaagttt
    32281 gctggaatcg ccatagccga tattaatcag cagattgctc ttcagcgtgc tgcccgcaaa
    32341 aaaggcatcg tcaacgtatt gacggtcaaa gcccgacatc gggccggtat ccagccccag
    32401 cgcacggcag gcgacgatca gataggccgc ctgcatggaa ctgttgcgaa acgctgtttc
    32461 ttcggcaagt tgtggactgg aggtaaacca actgcgggca tcgccgtgag gaaacagctg
    32521 tggtaaccgt tcataaaatt cactgtccca ggcgacgata gcggtgacgg gcgcggtcag
    32581 ggttttttgc agattgccgc tggaaagcgc cgggcgcaga cgttcttttc cttctgcagt
    32641 gcggataaac acgatccgtg ccggagaaca gttagcagat gtcggccccc atttcatcag
    32701 ggcataaatc tcccgtaacg tctcatcgct gacggggggc tcccgccagc cgttgtgagt
    32761 gcgggcatcg gtgaacaggg tgctaagcgc acctgggcta acggcttcgt tcatagcaat
    32821 tccttacaga gcggcttcac ggtgatgtaa caggctggca agcccgttga gtaacagagc
    32881 attaaacgtt tcgggatcgg tcacgttgca agcgtgtccg ccatagcgca tcaccatttt
    32941 ctggctatcg ggcagggcgg catgaagttc actggaacat gctgttggca ccagcagatc
    33001 atcactggcg cagatgattt gcaccgggca gcggatacga tccgcatggt gactaaagtc
    33061 agcgcgtttg agggcgttaa gtcgacgcag taaattattt ttgccttgaa aatgcgccag
    33121 tgccagcgcg tcttctgcct caaggcgagg tgcgcgggcc gccatccagt cggcgggata
    33181 gaggaacaac ggctgcgctt ccacccatgc ctgcgcgccg ccgctatgta gtaactgttc
    33241 gcgaacctga aaacagcggc gcgtatgggc gtttattcgt agccagccgt taacgctgac
    33301 cagcacagtt accgacgcgg gataatccag cgccagctgc attcccacca gcgcaccgag
    33361 cgcatggccg accaccgcgt aacgctcaat ccctgcggct accagcgcct gatgcagttc
    33421 cgctgccatc tgggcgatac tgtaatcttc tgccagcgtg tcgggattat tgccggtgcc
    33481 gcgctggtcg taacagacta cctgatactc ctgatccagc accgccagtt gcggtaacca
    33541 gtaactgccg ctaccgccaa gacccgaaat caacaccact acgggcgcat cagcataagg
    33601 gggaggtgag agtgaaagtt tcatcgcggc ctcacttggc gatatgcgca attgtggcga
    33661 tttccaccag cgcgtcaggt tttaccagtc cgcactgaat gcagaatcgc gccggtttgt
    33721 cacccgggaa aaactcggcg tagatttcgt taatcgcggc gtaatttttc cagtcggtaa
    33781 taaagatgct gttgaaggtc acatccgcca tcgtgccacc cgccgtctcg atgaccttgc
    33841 ggatggtttc cagaacgtgg cgggtttgcg cctttgggtc atcggcaaac agcacgttgt
    33901 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
    33961 gaacgaaggg ggccagcggt gcgctgctgc cagcaggaat aattacggat tttggcatcg
    34021 ttaaactcct taagcgatac gagcaaagga cgtggaagaa agcgcgtcgc agaacgtttc
    34081 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
    34141 ttccggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
    34201 accgtcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
    34261 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
    34321 cggcttcggc agcacaatat cgccaggctg cggcatcagt tcatccacca gttgataatc
    34381 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgattacgca tggttttcag
    34441 ggcgttcgat ttatgaaaat ttggtgagcc ggggccgcca gcctcgacat actgttcatc
    34501 ccagccattt tgaaaccaga tgatcagcat ccctgccgtt cgcgctgcgg tcacggcggt
    34561 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
    34621 gccgcctggc gtggcatagg cattttgcat atccaccacg atcagcgcag tttgctgcgg
    34681 atcgaaggta atggcttccg gtcgtgcggt taaggtcgtc atcatgccac ctcctgagtc
    34741 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaagttttcg
    34801 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
    34861 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaacgtccc catattgatg
    34921 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtctgtacc ggagcgggta
    34981 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
    35041 tgttcccatt tggcgcgagc ggcattgtcg gtttcgtcgg caatcaccat aaacaacaca
    35101 taagagccaa cgtcgcgtcc ggtttgctct gcggcctgtt tcatccgcgc agcggtcggg
    35161 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttaaaatc ggcataccgg
    35221 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
    35281 ggttgcggac tgacgcgaca atcatccata gtgaaaaaat cgcctttaaa atcgcttttc
    35341 cccgagcccc acaggtcgcg cagcacctga acatattcgg tgagatagtc gtaacgacgg
    35401 gagaaatagt catcgccagg ccagatcccc atctgctcat actcgggctt ctgccagcca
    35461 gtcacgaggt tgacgccaaa acgcccgcca gagatggagt cgatggttgc ggccatacgg
    35521 gcgacgattg ccggaggtaa cgttaaggtg gcagctgtcg cgtatatctg aatgcgtgag
    35581 gtgacggctg ccagccccgc catcaaggtg aacgactcaa ggttgtgatc ccagaactca
    35641 gttttgccgc caaagccacg cagtttgatc atcgacaggg cgaaatcgaa atggtagtgc
    35701 tccgcttttt gcacaatggc tttattcagt tcaaatgtcg gcatgtactg cggcgcgtgg
    35761 gtcgaaatga gccagccgtt gttgccaata ggtacgaata cgccaatttt catcatcaac
    35821 ctctcttcgt ctcgtaaagt gaaagtcaga cggggcgctg catcctgcat atccttttca
    35881 gccgcgtaat ggcttgtttg caaagcggat gccagttttt aaaaagtgaa tgttattaat
    35941 gtgttaatgt taagttgtct aaaatgccta aaaaaaagtg gactaaacgg tcaaaacagt
    36001 tgcacataaa acatgcattc gtgcgcgatg agagtgcaga aggtcgaggc cgggcggggg
    36061 ttttgctatc ctgttgccaa tcaacaagag gggagagcgc atgacgcaag gcgcagtgaa
    36121 aacaacgggt aagcgttcgc gcgcagtaag cgcgaagaaa aaagcgattc ttagcgcagc
    36181 actggacact ttttcacaat tcggttttca cggcacaagg ctggagcaga tcgcggagtt
    36241 ggcgggtgtt tcaaaaacca atctgctgta ttactttccg tcaaaagagg cgctgtatat
    36301 agccgtactg cggcagattc tcgatatctg gctggcaccg ttaaaagcgt ttcgagaaga
    36361 tttcgccccg ctggcggcga tcaaagaata catccgtctg aagctggaag tttcccgcga
    36421 ttatccgcag gcttcgcgcc tgttctgtat ggagatgctg gcaggtgcac cgctgttaat
    36481 ggatgaactg acgggcgatt tgaaggcatt aattgatgag aaatcggcgc tcattgccgg
    36541 ttgggttaaa agcggcaaac tcgcgccgat tgacccgcag catttgattt ttatgatttg
    36601 ggcttccact caacattacg ccgattttgc ccctcaggtg gaggcggtga caggcgcgac
    36661 gttgcgcgat gaggtgtttt tcaatcaaac ggttgaaaac gtgcagcgga ttattattga
    36721 ggggattcga ccacgttaaa gatgccggag gaggttgtaa catcctccgg ctacctgttt
    36781 aaccgatcgt cattaaactg gcgttaccgc cagcagcagc ggtattcaca ctcagcgaac
    36841 gctcgatata cagccgttcc agaaggatat tgctttcgcc acgggcaaaa ccctgcaccg
    36901 aaacaattgc accatcccgt gcggcaacgg cttcacacaa tgcgcgaagc tgatccgaat
    36961 caccgtggaa gatcaccgca tcaaatggtt gagcggttat attttccgct ttcgccagtt
    37021 gaatacgttc gctgactgcc gatggcaatg ccttcactaa ctgacgatgc agcgcatcat
    37081 ccggccacag cacctggcta cccaccgcca gcacggcggc gagctgagtc agcgcatcct
    37141 gttcatcatc cgcaatacac aacacgcgct cacgcggcag cagcgtccag gtgttgcgtt
    37201 cacccgtcgg ccctggcaac aatcgttgtg ttcctgcctg cgccaactcg ccatattgcg
    37261 tacataacgc ctgcaattct ggacggtttg ccgcccattc ccgcagtgca tttagcggct
    37321 gagtcaatgc ggctttcaac tgcgcatcga ccggatactc cgcatcctga cgcgcgagcg
    37381 tcaccgccag cgcactttcc gggcgattcg ccagcagacg gtagagatag agcggaccgc
    37441 ctgctttcgg cccggtaccg gacaaccctt cgccgccgaa cggttgcacc ccaaccaccg
    37501 cgcccaccat attacggtta acgtacagat taccgacctg ggccgagcca gtgacctggg
    37561 caatggtttc atcaatacgc gtatgaacgc caagcgtcag accatagccg gaagcgttaa
    37621 tctgctcgat cagctctggg agctggttac ggttgtaacg caccacatgc agcaccggac
    37681 caaagacctc tttttgtaat tcggcaaagt catccagttc gatcagcgtc ggagcgacaa
    37741 aggtgccgct ttgccattca cgggcatctt cgctgttttc ccgcaccgcc tggaacacct
    37801 ggcggccttt actacgcatg gtctgaatat gacgctcgat attggctttc gcttcgctat
    37861 caatcaccgg accgatatcg gtggtcaggc gacccggatt acccatccgg cattcggcca
    37921 ttgcgccgcg cagcattttc agcgtgtggt cggcaatctc atcttgcagg cacagcacgc
    37981 gcagtgccga acaacgctga cctgcactgt cgaacgccga ggccagcaca tctattacga
    38041 cttgttcggt cagtgctgaa gaatcgacaa tcatcgcgtt catgccgccg gtttccgcaa
    38101 tgagcggaat tgggcgaccc tgagcgtcca ggcggctggc gatattgcgt tgcagtaacg
    38161 tagcgacttc ggttgaaccg gtaaacatca ccccgcgcac gcgttcatca cccgtcagtt
    38221 gcgcgcccac ggtttcaccc cgacctggca gcaattgcac cacgcctggc ggtacacccg
    38281 cttccagcaa aatggcgatc ccttgcgcgg caatcagcgg cgtttgttct gccggttttg
    38341 ccagcacgct gttacctgcc gccagtgcgg cagcgatctg cccggtgaaa atagccagcg
    38401 ggaagttcca cggactgata cataccacag gccctaatgg acggtgggtt tcgttagcga
    38461 aatcatcccg cacctgtccg gcatagtagt ggagaaaatc gaccgcttcg cgcacttcgg
    38521 caatggcgtt actgaaggtt tttccggctt cacgcaccag aataccaatc agttgctgca
    38581 tctggctttc cattagcacg gcagcgcgat gcaaaatcgc cgcgcgttca gccggaggag
    38641 tggcaaacca gattggcgcg ttattaaccg cactttccag cgcctgttct acttcacgcg
    38701 gcgtggcttc acgcacatag cccacaatat ctttcggttc cgcagggtta ataacgggcg
    38761 acatctcacc agccgcgacc gattgttcca gcattggcaa ggcctgccat ttttgcagtg
    38821 cactattgag cagggcagag gagagcgagg ccagacggtg ttcgttagcg agatccagcc
    38881 ctgccgagtt gtcgcgcccg tgaccgtaaa gatcgcgcgg caggggaatt ttcggatgcg
    38941 gtaatccagt ttgcccttcc tgttgcgcca gtttttctac agcagtgact ggatcggcga
    39001 ccagttcatc cagtggcaaa gaggtgtcgg caatacggtt aacaaacgag gtgttagcac
    39061 cgttttccag caggcggcgc accagatatg ccagcagcgt ttcatgtgta ccgaccggag
    39121 cataaatacg acacggacgg ttaagtttgc cgtcggcaac tttcccggtg acctgctcat
    39181 acagtggctc gcccatacca tgcaggcact ggaactcgta ctgacccggg tagtagtttt
    39241 gacccgccag ttgataaatt gccgccagcg tatgggcgtt gtgcgtcgcg aactgcgggt
    39301 agattagatt tggcaccgcc agcagttttt tcgcacaggc aagataagaa acgtcggtat
    39361 acaccttgcg ggtataaacc ggataacctt caaggccgtc catctgtgca cgcttgattt
    39421 cactatccca gtacgcgcct ttcaccaggc gaatcatcag acggcgacgg ctgcgggtgg
    39481 cgagatcaat caggtaatcg atcaccaacg ggcagcgttt ttgataagcc tgaataacaa
    39541 aaccgatgcc gttccagcct gccagttcag gctcgaaaca gagtttttcc agcagatcga
    39601 gggagatctc cagacgatcg gcctcttcgg catcgatgtt aataccaata tcgtactgac
    39661 gcgccagcag ggtgagtgat ttcagacgcg ggtaaagctc ttccattacc cggtcatact
    39721 gggcgcggct ataacgcgga tgtagcgccg acagcttgat tgaaatgccc ggaccttcat
    39781 agatgccacg accgttagac gctttaccaa tggcgtgaat cgcctgctga taggaaacca
    39841 tatacgcttg tgcatctgcg gcagtcagtg cggcttcgcc cagcatatcg taagagtaac
    39901 ggaaaccttt ctcttccagt ttgcgggcat tggctaacgc ttccgcgatg gtttcgcccg
    39961 tgacgaactg ctcgcccatc aggcgcatcg ccatatccac acctttgcgg atcagcggtt
    40021 caccgctctt accgataatg cggttcagcg agcgggagag gctggcttcg ttatgggtgg
    40081 aaaccagttt gccggtaaac agcagccccc aggtagcggc attaacaaac agggacgggc
    40141 tacgaccaat gtgtgactgc cagttaccgt tactgatttt gtcgcgaatt aacgcgtcac
    40201 gggtggcttt gtcgggaata cgcaacaacg cttccgccag acacatcagc gccacgcctt
    40261 cctgcgatga caacgaaaac tcctgcaata acccctgaac catacctgcg cgaccactgg
    40321 cgtttttttg attacgcagt ttatcggcca gttgatacgc cagtttgtgc gcctgttcag
    40381 ctactggctg cggcaggcga gcttgttcca gcagcataga aaccgcttcg gtttccgggc
    40441 ggcgataggc ggcggtgatg gcggcgcggg aaaccgactg gggcaatatt tgctcggcaa
    40501 agtcgaggaa aggctggtgt ggttcctctg ccggagtcgg tgcttcatcg ctctcattgg
    40561 ccgcgccaga aagcagcgct ggtagctccg ggagagtatc gctgttttcc agttgttcaa
    40621 gataagaaaa aatcgcctgt ttaattaacc agtgtggtgt gcgatcgata cgtgtcgcgg
    40681 cagacttaat acgctcacgc gtcgcgtcgt ccagcttaac ccccatggtg gtggttccca
    40741 tgccattact cctgttgttc tgaaaggtgc aacttaacgt tatcgtgaaa tatccatgat
    40801 gttgcaactt tgtgcaaccg tgttaaatgt gacctgcgta gcaagcttaa aaatgaatga
    40861 aatgttaata aacgaaatcg atatgacagg gattaaaaaa ataactcaga ctttttctct
    40921 gcggcagtta acatttttga aaggtgcaac cgcaaaaaat gtgagagagt gcaacc
//
LOCUS       DAZFRG010000011        40348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFRG010000011 DAZFRG010000000
VERSION     DAZFRG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.011"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..1253
                     /locus_tag="ACHKUC_000423"
     CDS             84..1253
                     /locus_tag="ACHKUC_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFO6616747.1"
                     /translation="MSVALIRDGVIRPSENDSHMLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVALF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            1250..1720
                     /locus_tag="ACHKUC_000424"
     CDS             1250..1720
                     /locus_tag="ACHKUC_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6616748.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            1734..3014
                     /locus_tag="ACHKUC_000425"
     CDS             1734..3014
                     /locus_tag="ACHKUC_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6616749.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATILETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIDVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(3103..5049)
                     /gene="nagE"
                     /locus_tag="ACHKUC_000426"
     CDS             complement(3103..5049)
                     /gene="nagE"
                     /locus_tag="ACHKUC_000426"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO6616750.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            5382..6182
                     /gene="nagB"
                     /locus_tag="ACHKUC_000427"
     CDS             5382..6182
                     /gene="nagB"
                     /locus_tag="ACHKUC_000427"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6616751.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLSKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            6242..7390
                     /gene="nagA"
                     /locus_tag="ACHKUC_000428"
     CDS             6242..7390
                     /gene="nagA"
                     /locus_tag="ACHKUC_000428"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6616752.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            7399..8619
                     /gene="nagC"
                     /locus_tag="ACHKUC_000429"
                     /gene_synonym="nagR"
     CDS             7399..8619
                     /gene="nagC"
                     /locus_tag="ACHKUC_000429"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO6616753.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            8667..9419
                     /gene="nagD"
                     /locus_tag="ACHKUC_000430"
     CDS             8667..9419
                     /gene="nagD"
                     /locus_tag="ACHKUC_000430"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO6616754.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            9675..11339
                     /gene="asnB"
                     /locus_tag="ACHKUC_000431"
     CDS             9675..11339
                     /gene="asnB"
                     /locus_tag="ACHKUC_000431"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO6616755.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            11720..11796
                     /locus_tag="ACHKUC_000432"
     tRNA            11720..11796
                     /locus_tag="ACHKUC_000432"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11754..11756,aa:Met,seq:cat)
     gene            11807..11891
                     /locus_tag="ACHKUC_000433"
     tRNA            11807..11891
                     /locus_tag="ACHKUC_000433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11841..11843,aa:Leu,seq:tag)
     gene            11915..11989
                     /locus_tag="ACHKUC_000434"
     tRNA            11915..11989
                     /locus_tag="ACHKUC_000434"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11947..11949,aa:Gln,seq:ttg)
     gene            12024..12098
                     /locus_tag="ACHKUC_000435"
     tRNA            12024..12098
                     /locus_tag="ACHKUC_000435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12056..12058,aa:Gln,seq:ttg)
     gene            12114..12190
                     /locus_tag="ACHKUC_000436"
     tRNA            12114..12190
                     /locus_tag="ACHKUC_000436"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12148..12150,aa:Met,seq:cat)
     gene            12239..12313
                     /locus_tag="ACHKUC_000437"
     tRNA            12239..12313
                     /locus_tag="ACHKUC_000437"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12271..12273,aa:Gln,seq:ctg)
     gene            12351..12425
                     /locus_tag="ACHKUC_000438"
     tRNA            12351..12425
                     /locus_tag="ACHKUC_000438"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12383..12385,aa:Gln,seq:ctg)
     gene            complement(12580..13755)
                     /gene="ubiF"
                     /locus_tag="ACHKUC_000439"
     CDS             complement(12580..13755)
                     /gene="ubiF"
                     /locus_tag="ACHKUC_000439"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO6616756.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMAENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            13901..15325
                     /gene="miaB"
                     /locus_tag="ACHKUC_000440"
     CDS             13901..15325
                     /gene="miaB"
                     /locus_tag="ACHKUC_000440"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO6616757.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            15478..16518
                     /gene="ybeZ"
                     /locus_tag="ACHKUC_000441"
     CDS             15478..16518
                     /gene="ybeZ"
                     /locus_tag="ACHKUC_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO6616758.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            16515..16982
                     /gene="ybeY"
                     /locus_tag="ACHKUC_000442"
     CDS             16515..16982
                     /gene="ybeY"
                     /locus_tag="ACHKUC_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO6616759.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            17072..17950
                     /gene="corC"
                     /locus_tag="ACHKUC_000443"
     CDS             17072..17950
                     /gene="corC"
                     /locus_tag="ACHKUC_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO6616760.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17975..19513
                     /gene="lnt"
                     /locus_tag="ACHKUC_000444"
     CDS             17975..19513
                     /gene="lnt"
                     /locus_tag="ACHKUC_000444"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO6616761.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(19640..21517)
                     /locus_tag="ACHKUC_000445"
     CDS             complement(19640..21517)
                     /locus_tag="ACHKUC_000445"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO6616762.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYTLRESEQIVISIGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVGQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNNLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            21971..22879
                     /gene="gltI"
                     /locus_tag="ACHKUC_000446"
     CDS             21971..22879
                     /gene="gltI"
                     /locus_tag="ACHKUC_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO6616763.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMSFELSDEMKALFKEPNDKALN"
     gene            23049..23789
                     /gene="gltJ"
                     /locus_tag="ACHKUC_000447"
     CDS             23049..23789
                     /gene="gltJ"
                     /locus_tag="ACHKUC_000447"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO6616764.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            23789..24463
                     /gene="gltK"
                     /locus_tag="ACHKUC_000448"
     CDS             23789..24463
                     /gene="gltK"
                     /locus_tag="ACHKUC_000448"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO6616765.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            24463..25188
                     /gene="gltL"
                     /locus_tag="ACHKUC_000449"
     CDS             24463..25188
                     /gene="gltL"
                     /locus_tag="ACHKUC_000449"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO6616766.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            25306..26241
                     /gene="rihA"
                     /locus_tag="ACHKUC_000450"
     CDS             25306..26241
                     /gene="rihA"
                     /locus_tag="ACHKUC_000450"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO6616767.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(26286..26768)
                     /gene="ybeL"
                     /locus_tag="ACHKUC_000451"
     CDS             complement(26286..26768)
                     /gene="ybeL"
                     /locus_tag="ACHKUC_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO6616768.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            27003..29585
                     /gene="leuS"
                     /locus_tag="ACHKUC_000452"
     CDS             27003..29585
                     /gene="leuS"
                     /locus_tag="ACHKUC_000452"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO6616769.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPIADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            29600..30181
                     /gene="lptE"
                     /locus_tag="ACHKUC_000453"
     CDS             29600..30181
                     /gene="lptE"
                     /locus_tag="ACHKUC_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO6616770.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTLATPARVSTTLGN"
     gene            30181..31212
                     /gene="holA"
                     /locus_tag="ACHKUC_000454"
     CDS             30181..31212
                     /gene="holA"
                     /locus_tag="ACHKUC_000454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO6616771.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            31214..31855
                     /gene="nadD"
                     /locus_tag="ACHKUC_000455"
     CDS             31214..31855
                     /gene="nadD"
                     /locus_tag="ACHKUC_000455"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO6616772.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSAQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            31879..32490
                     /gene="cobC"
                     /locus_tag="ACHKUC_000456"
     CDS             31879..32490
                     /gene="cobC"
                     /locus_tag="ACHKUC_000456"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO6616773.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            32750..33067
                     /gene="rsfS"
                     /locus_tag="ACHKUC_000457"
     CDS             32750..33067
                     /gene="rsfS"
                     /locus_tag="ACHKUC_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO6616774.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            33071..33538
                     /gene="rlmH"
                     /locus_tag="ACHKUC_000458"
     CDS             33071..33538
                     /gene="rlmH"
                     /locus_tag="ACHKUC_000458"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO6616775.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            33569..35470
                     /gene="mrdA"
                     /locus_tag="ACHKUC_000459"
     CDS             33569..35470
                     /gene="mrdA"
                     /locus_tag="ACHKUC_000459"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO6616776.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            35473..36585
                     /gene="mrdB"
                     /locus_tag="ACHKUC_000460"
                     /gene_synonym="rodA"
     CDS             35473..36585
                     /gene="mrdB"
                     /locus_tag="ACHKUC_000460"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO6616777.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            36596..37684
                     /gene="rlpA"
                     /locus_tag="ACHKUC_000461"
     CDS             36596..37684
                     /gene="rlpA"
                     /locus_tag="ACHKUC_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO6616778.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVIPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            37824..39035
                     /gene="dacA"
                     /locus_tag="ACHKUC_000462"
     CDS             37824..39035
                     /gene="dacA"
                     /locus_tag="ACHKUC_000462"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO6616779.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            39145..39408
                     /gene="ybeD"
                     /locus_tag="ACHKUC_000463"
     CDS             39145..39408
                     /gene="ybeD"
                     /locus_tag="ACHKUC_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO6616780.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            39509..40150
                     /gene="lipB"
                     /locus_tag="ACHKUC_000464"
     CDS             39509..40150
                     /gene="lipB"
                     /locus_tag="ACHKUC_000464"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO6616781.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         9804 a        10528 c        10392 g         9624 t
ORIGIN      
        1 aaaaaagttt cgacattgat cgaaacgcat gaatcgcact cgcattaata aatctattac
       61 caactttaag atggagtcta actatgtctg tagcccttat tcgtgacggc gtcatccgtc
      121 catccgaaaa cgattcgcat atgcttaccg ccatgctgcc ctcgtcctgc ccgcaaaacc
      181 acgcggcgaa tattctgcct ttgccggatg gcgcgttaat gtgcgtctgg tttgcgggaa
      241 cgcaggaagg tattgccgat atttctgtct ggggatcgcg attgcccgct ggcggaatgc
      301 agtggagcga tgcggtcaaa ctttcccacg atgacacgcg ttcagagcaa aacccggtgc
      361 tgtttctcgc gccggataac gtgctgtggc tcctatggac cgcacaaata tccggtaatc
      421 aggacaccgc catcgtccgc taccgtaagt cagacgacct cggacaaacc tggggcgaga
      481 ttgccaccct gcttgataaa cccggtacgt tcattcgaca gccaatcacg gtgcttgata
      541 acggcaactg gctgctgccc gtcttttatt gccgtaccca acccggcgaa aaatgggtcg
      601 gcaatgatga tatcagcgca gtgaaaattt ccgctgacgg tggtcactcc tggcgcgatg
      661 tcgaggtgcc gcaaagtctt ggctgtgttc atatgaatat cacaatgctg cacaacggta
      721 cgttggttgc tctctttcgc agccgttggg cagacaacat ctatatcagc cattccgcgg
      781 acaacggcga gagctggtca gtaccgcaag caaccgaatt gcccaacaac aattcgtcta
      841 ttcaggtaac gacacttgcc agcggcgaac tggcgctggt gtacaacgcg atgagtgcgg
      901 caggcgcagt agaacgtcgc gcgtcgcttt atgacgaaat cgacgatggc gacgacagcc
      961 gcaaagaacc gacagccgtc ggacgctccg ctttctgggg ggccccacgc gcgccgatga
     1021 cggtagcgat ttctgccgat ggtgggaaaa gctggccgtg gcggcgcaat ctcgatgaag
     1081 gcgacggcta ctgcatgacc aacaattccc tggaaaagct aaatcgtgag ttctcctacc
     1141 ccagcatcaa acaaagcccg gacggtacgc tacatatcgc ttacacctgg tggcgtcagg
     1201 ccatcaagta cgtacgcatc tcccccgagt gggttaaggg gcaggcatca tgattatcgg
     1261 taatctcaac catttgtcgc tggcggggct tcctgcctgg gtgagaaata ttctgctgcg
     1321 cccggagtgt tcgttatcgg cgttatccac cagagaggac ggtcgctggc aacctgaagg
     1381 atgtcggtgg ttctgcacgc tgggaacttc agacacgcaa cccgccgaac tccgccatac
     1441 ggagtatcac catttatggg ccgatataca ggtggtgatt actggttgtg agggcattaa
     1501 tgcgggaacg cggcccatcg ctcgcgaaaa cgatgaagaa cgtaagcccg atctgtttat
     1561 cgccccatcc cctgaaaaca gtgtcgccat caccctacac gccggtgatt tcgccgtctt
     1621 tatgcctgag gaacctcacc aggcgctgtg cgcaattggc acggctactt caatacgcaa
     1681 agcggtcttt aaagtaccgc gcgacatgct ggaagcctaa aaggagagaa aaaatgacaa
     1741 tggaaatgat cctggcatta ggaatactgg tcctgatgat cgtgctgatc atgtcggaca
     1801 aaatgccttt tggcgcaccg ccactgcttg cgtgtttatt gctggttgta tcgggacttt
     1861 cgaccgttca acaggctttt gccggattcg tcaatccaag cgtggtgatg attgcagggt
     1921 ttatggtggt catggcggcg ctacaaaaaa cccggcttat cagcaacgtt aaatctgcga
     1981 tgataagcct ggtcaataaa ggcagttacc gcagctatgg gctgttgtta gtcattgtta
     2041 tgctgggtgc cagtcttgcc ggcaccggag cgaccggtta ttacgtgcta attctgtcgc
     2101 tggtatccac cattccgtac agtaaaaagc taccaacatc gaaattaatg atgcccctgg
     2161 gatttgccac caaccacccg cttcttccta tcaatctcgc gcttctcttt ggcgttacgg
     2221 cgaccatact cgaaaccgcg gggtttcatc aggagatttc gatggggcgc tttgcgctgg
     2281 tgaatctcat catgtcagcg gctttcctcg catggagtct tattgcctac cgttttcttc
     2341 cggatcatcc cattgcggat gcttccgagg atgcgctggc ggcacgcgag gaaacgttta
     2401 acgcccttcc tgcctggaaa gaatattgca ctattgccgc attcgctgtc agcgttatcg
     2461 gcatgatgct aatgaacatt ctgggaaata tcgccttcgt tattccgggt cttgcgggtg
     2521 cgtttgttct gatgatcgat gtactggact tcaaagaagt tcgcgatcat atgggggctc
     2581 cggtcatttt aatgatggcg ggggtgatcg atgtagctga tgcactggcg ggcaccggct
     2641 ttacggcgat ggttggcgat gccgttgcag gtgtgctcgg ttctggcgtc tcaccgtttg
     2701 tatttattgt cgcctttgct ttgcttacca gtacctgcgc caccttcacg ggttcgaata
     2761 tggggtcagt atatattttt gcccccatcg ccattgccgc ctgtactagc cttgggctta
     2821 acccgaccgc tgccgcgatt gcggtggtga tttctggctg gaatggcggc tatatgccta
     2881 ttgacggaat gccagccatg atcctcggaa tggggaaata caaacttccc gaattctggg
     2941 tgttctctgt accaatgtat ctcatccgca ttctggcgct ttgtgcaggc gctgttttta
     3001 tcttcccaat gtaatctcag acaaaaaaag cggcagagaa gatccctgcc gctttttatt
     3061 gttggatgcg acgctgccgc gtcgcatcag acataaagca gattactttt tgatttcata
     3121 cagcggtgtt tgacccgcca caacatggcc ctgagcttta atgataaggc cgctgaaatc
     3181 gtcgatattg ctgcaaacca ccgggctaat catcgagcga gcgttagcgt tcaggtaatc
     3241 cagatccatt tccagaatcg gttgccctgc gcttacctgc gcgccctctt ccaccagacg
     3301 tttaaagcct ttaccttcca gcgctacggt gtcgataccc atatggacga cgatctccgc
     3361 gcctttttcg gtttccagac agaatgcgtg gttggtgttg aagattttca cgattgttcc
     3421 tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc
     3481 tttgctggcg aatgcttcgt caggaacctg atccagtgcc acaacatccc cggtaatcgg
     3541 cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggagc
     3601 ggcagttgcc ggagttgctt cagctgacgc agcggctacc ggaccacggg caacgacttt
     3661 cttcatcgca tcgccgatgg attctgcttt cgcgccaaca atcacctgaa tagtctgttt
     3721 gttcagtttc actaccccag aagcacccag acgtttacac atcgcatcgt tgacgcgagc
     3781 tgagtcaacc acggtaaggc gcagacgagt aatacaggcg tcaatggctt tcaggttgtc
     3841 agtaccgcca accgcagcaa tatagttggt tgccagttga ttgagacctt cttcagtatt
     3901 gctgttagcc tcttcagtaa cgatctcgtc ttctttatct tcacgacccg gcgttttcag
     3961 gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga agacaacccc
     4021 catcaccagc agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta
     4081 gtcgatagcc cccgcagaga aagagaagcc ggcatggata cccagcagcg ttgccacaaa
     4141 caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggtgcaa ggaacatgaa
     4201 caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag
     4261 catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc
     4321 acccggcaga ccgaacatca taatcgggaa gaagccggac atgaacatcc ccgcggtgcc
     4381 gtcaccggca tagaagcggt tgatatcacc gtggaaaacc gttcccgccg cgttggtgaa
     4441 ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag
     4501 cagacggttg atgaaaccaa agataccgga acccagcgcg cccgcagaaa cgatccactc
     4561 gccgcctgca tggatagcgt gctgtaccgg tggccagacg taaccaaaaa tggccgccag
     4621 caccagacag aagaagccgg tggcgatcgg cacaaagcgt ttgccgccga agaagctcag
     4681 gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt
     4741 aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca tcgctttggt
     4801 taacacaaag taacctaccg cacccgccag tgccgccgca cctgcgctgt ctttcgacca
     4861 gctggatgcc acgccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc
     4921 ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagcgccgc
     4981 caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa
     5041 aatattcatc ttattccccc tacgagaatc ctatttggct cgtttaaagc cgtattttta
     5101 ttttgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag
     5161 agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt gtcttttgtg agttttgtca
     5221 ccaaatatcg ttatcatcac tcccttttac tggctaaacc agaaaactta ttttatcatt
     5281 caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct taaagatgcc
     5341 taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctga
     5401 ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt
     5461 tcaaaccgac tgccgatcgt ccgtttgtac tgggcctgcc gactggcggc acgccgatga
     5521 ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg
     5581 tcaccttcaa catggacgaa tatgtcggtc tgtcgaaaga gcatccggaa agctactaca
     5641 gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaat atcaaccttc
     5701 tcaacggcaa cgccccggat atcgacgccg aatgccgcca gtatgaagaa aaaatccgtt
     5761 cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat attgcattta
     5821 acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc
     5881 gcgtcgcgaa ctctcgtttc tttgataacg atgttaatca ggtgccaaaa tatgccctga
     5941 ctgtcggtgt tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagtc
     6001 agaaagcact ggcactgcag gccgccgttg aaggttgcgt gaaccacatg tggaccatca
     6061 gctgtctgca actgcatcca aaagcgatca tggtgtgcga tgaaccttcc accatggagc
     6121 tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt
     6181 aattattatc cctgccctga caccttgctc agggcaatat tttttaaaat cgggggtcag
     6241 aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc
     6301 ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga
     6361 gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt
     6421 aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct
     6481 ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat
     6541 caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaaa
     6601 acatccgaac caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa
     6661 aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg
     6721 tgagaacgcc gacgtgatta ccaaagtgac tctggcaccg gaaatggttc cagcggaagt
     6781 catcagcaaa ctggcaaatg ccgggattgt ggtttctgct ggtcactcca acgcgacgtt
     6841 gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc
     6901 gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga
     6961 catttattgc ggtattattg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc
     7021 taagcgtctg aaaggcgaca aattgtgtct ggttaccgat gccaccgcgc cagcaggtgc
     7081 caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaatg gcctttgtgt
     7141 ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct
     7201 ggtcgaacat tgtggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc
     7261 acgtgcgatt ggcgttgaga aacgtctcgg cacgctcgcc gcaggtaaag tagccaacct
     7321 gactgcattc acacctgatt ttaaaatcac caggaccatc gttaacggta acgaggtcgt
     7381 aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc
     7441 gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg
     7501 cggattcaga ttgccgagca aagtcagctt gcccccgcca gcgtaaccaa aattacgcgt
     7561 cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc
     7621 cgcgctatct ccatcgtcac cgaaacccgc aatttccacg caatcggcgt acggcttggt
     7681 cgtcacgacg ccaccatcac cttgtttgat ctcagcagca aagtgctggc agaagaacat
     7741 tacccgctgc cggaacgtac ccagcagacg ctggaacatg ccctgctgaa tgccattgct
     7801 cagtttattg atagctacca gcgcaagcta cgcgagctga tcgcgatttc ggtgatcctg
     7861 ccagggcttg tagacccgga cagcggcaaa attcattaca tgccgcatat tcaggtagaa
     7921 aactgggggc tggttgaagc actggaagag cgttttaaag tgacctgttt tgtcggccac
     7981 gatatccgca gtctggcgct ggcagagcac tacttcggtg caagtcagga ttgcgaagac
     8041 tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa cgggcgaatt
     8101 tttatcgggc gcaacggcaa cgtcggtgaa attggccata ttcaggtcga accgctgggt
     8161 gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgccgccaa tgctgccatt
     8221 gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgtgtgcc actggacgac
     8281 tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagcctggc ctcggaagtg
     8341 attgaatatg tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa cttatttaat
     8401 ccgcaaaaaa ttgttattgc tggagaaatc accgaagccg ataaagtgct gctccctgct
     8461 attgaaagct gcattaatac ccaggcgctg aaggcgtttc gtactaatct accggtggta
     8521 cgttctgagt tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg
     8581 ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt
     8641 attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgacg
     8701 gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta
     8761 tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc gggcaagatc
     8821 tggcgaatcg ctttgccact gccggtgtcg atgtaccgga tagcgtgttt tatacctccg
     8881 cgatggcgac cgccgatttt ctgcgtcgcc aggaaggcaa gaaagcgtat gtggtgggcg
     8941 aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg
     9001 attttgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat aaagcagcct
     9061 atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac gggcgcggtt
     9121 tttatcccgc ctgtggcgcg ttgtgtgcag ggatcgagaa aatctccggg cgcaaaccgt
     9181 tctatgttgg aaagccgagt ccgtggatca tccgcgcggc attaaacaaa atgcaggcgc
     9241 attcggaaga aacggtgatt gtcggcgata acttgcgtac cgatattctg gcgggcttcc
     9301 aggccggtct ggagacgatt ctggtgcttt ctggtgtttc gtcgctcgac gatatcgaca
     9361 gtatgccgtt ccgcccaagt tggatttacc catcggtcgc tgaaatcgac gttatctgaa
     9421 tagcaaccac gcctcagggc gtggtttctt atttttaccc atcattaaaa tcaccctatt
     9481 caataaaacc cgtcaaaaat tatcgatcca tcaataaata cgccatcaca ccctatcctt
     9541 tttcacaaat cagcaatcgc cattacgttt tttatttttc caccgccaaa tcgcttgcgc
     9601 accgtgctgc tttgcggcat tttttaaaca agcaaacaca acaagcaaca aataccaggt
     9661 taacggagaa ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca gacgcagttg
     9721 agctgcgtaa gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt
     9781 ccggcattta tgccagcgat aacgccattc tcgcccacga acgtctgtca attgttgacg
     9841 ttaacgcggg ggcgcaacct ctctacaacc aacaaaaaac tcatgtgctg gcagtaaacg
     9901 gtgaaatcta caaccaccag gcattgcgcg ctgaatatgg cgatcgttac cagttccaga
     9961 ccgggtctga ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg
    10021 acgacttgca gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcatatctga
    10081 ttggtcgcga ccatctgggg atcatcccac tgtatatggg ctatgacgaa cacggtcagc
    10141 tgtatgtggc ctcagaaatg aaagccctcg tgccagtttg ccgcacgatt aaagagtttc
    10201 cggcggggag ctatttgtgg agtcaggacg gcgaaatccg ttcttattat catcgcgact
    10261 ggttcgacta cgatgcggtg aaagataacg tgaccgacaa aaacgagctg cgtcaggcac
    10321 tggaagattc cgttaaaagc catctgatgt ctgatgtgcc ttacggtgtg ctgctttctg
    10381 gtggtctgga ttcctcaatt atttccgcta tcaccaagaa atacgccgcc cgtcgcgtgg
    10441 aagatcagga acgctctgaa gcctggtggc cgcagttgca ctcctttgct gtaggtctgc
    10501 cgggttcacc ggatctgaaa gcagcccagg aagtggcaaa ccatctgggc acggtgcatc
    10561 acgaaattca cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca
    10621 tcgaaactta tgatgtgacc actattcgcg cttcaacacc gatgtattta atgtcgcgta
    10681 agatcaaggc gatgggcatt aaaatggtgc tgtccggtga aggttctgac gaagtgtttg
    10741 gcggttatct atacttccac aaagcgccga acgctaaaga gttgcatgaa gagacggtgc
    10801 gtaaactgct ggccctgcat atgtatgact gcgcgcgtgc caacaaagcg atgtcagcct
    10861 ggggcgtgga agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca
    10921 ttaacccaca ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat
    10981 gttttgaagc gtatctgcct gcaagcgtgg cctggcggca aaaagagcag ttctccgatg
    11041 gcgtcggtta cagttggatc gacaccctga aagaagtggc agcgcagcag gtttctgatc
    11101 aacagctgga aactgcccgc ttccgcttcc cgtacaacac gccgacctca aaagaagcgt
    11161 atctgtaccg ggagatcttt gaagaattgt tcccgcttcc gagcgccgct gagtgcgtgc
    11221 cgggcggtcc ttccgtcgct tgttcttccg ctaaagcgat tgagtgggat gaagcgttca
    11281 agaaaatgga cgatccgtct ggtcgcgcgg ttggcgttca ccagtcggca tataaataag
    11341 acgtgtaata ttgccccgga gaaatccggg gcaatttcag ttactggggc gcttgttgat
    11401 tctgatatgc agtaaggaaa tgttccgggt ttgccacatc gctgtttgac aactcattac
    11461 tgtaggccag tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc
    11521 ctgtatgaca aaagaatggc tgtagggtgg ggaaaaaaca caaagtaaac aataattgac
    11581 gaatatagcg ccatgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt
    11641 gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag
    11701 gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc actcataatg
    11761 atggggtcac aggttcgaat cccgtcgtag ccaccatctt ttttttgcgg gagtggcgaa
    11821 attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc
    11881 ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc ggtaaggcac
    11941 cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat attcttcgaa
    12001 taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc ggtttttgat
    12061 accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa tctggctacg
    12121 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    12181 gtagccacca aattctgaat gtgtcgaata tgttcggcaa attcaaaaac caatttgttg
    12241 gggtatcgcc aagcggtaag gcaccggatt ctgattccgg cattccgagg ttcgaatcct
    12301 cgtaccccag ccaatttatt caagacgctt accttgtaag tgcacccagt tggggtatcg
    12361 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    12421 agccacatta aaaaagctcg cttcggcgag ctttttgctt ttctgcgtat attcaatgcc
    12481 gaatgcgatg ttgactcgtc ttatccttca aagtcggatg cgacgctgcc cgcgtcttat
    12541 ccgacctacg gttggcatgc atccagcaat gttgtgaggc tacaacccta acgcatattt
    12601 cagcgcctga cgtttcaaca cgccagcacg ctccgccgcc atcaaaccaa gattacgcac
    12661 aaaacgcagc ggtggcagat tattgctgaa tccggcataa aacagatcca taccactttg
    12721 catcatgaag ttatccgcca tacgccgcat ctggtaacgc ttgagcacag gataactggc
    12781 ccacgcttcg ccgtagctgc gggcgttcac cagaacgtca atcagggcat cgacatcacg
    12841 ataaccaaga ttcactccct gccccgccag cggatggatg gtatgcgcgg catcgcccac
    12901 cagagcaagc cctggttgca catactgcaa cgcatggcga cgtatcagcg gaaacgcacc
    12961 agcggcaagc ggtgttacgt aacccagacg cgacgggaaa tgcttcgcga tttccgcctg
    13021 tagctgcgcc atattcatat tctgcaactg gcgaatgcgc gccggagagt cataccacac
    13081 cagcgatgcc cagttatcaa acaacggcag aaacgcacgc ggtccgtccg gggtaaattg
    13141 ctgccaggtg ctgtcgccgg gatcgttctc acactgtacg ctaattaaca tacacgactg
    13201 cgcatactgc caggcatgaa cgccaattcc cgccatctgt cgcacctgcg aatttgcgcc
    13261 gtcggcacca atcaccagct tcgcgaggat cgtttcaccg cccttcagtt ccagctcctg
    13321 aagatcatta tggcgatgca gtgcaatcag cgaggttggc acacgtaacg ttactttcgg
    13381 atgcgcttcc agcgcctgcc acaacgcctg ttgcaggaca gtgttttccg ccatgtagcc
    13441 aagcaacggt agcttaagtt cagcggcgtc aaacacgaca tgcgccgttt cccactccca
    13501 cgtttccagt ctgcggtaag gatggcaacg catagcctgt actgcatccc agacccctaa
    13561 ccctttaagc aatgataccg aagccgcgct gatcgccgag atccgcacgt ccggctggct
    13621 gtccgcaaca aacggcgcag gctgggcatg ttcaataacc gttaccgtaa acccgtgctg
    13681 tgccagtccc agcgccagcg cgccgccgac cattcctccg ccgacaatgg caatttccgt
    13741 tggttgattt gtcatggtca atcatcctgt tagcaatatg ctttaagttt acaggatttt
    13801 tacccccaag ggctgataac gctcatactg gtcacaacgg cagcaaagca ttacactatg
    13861 cgccctgcat tcctggctac tatttcgcaa gagcaagtcg atgaccaaaa aactccatat
    13921 taaaacctgg ggctgtcaga tgaacgagta cgattcatcg aagatggccg atctgctgga
    13981 tgccacccac ggctatcaac tgaccgacgt ggcggaagaa gcggatgtgc tgctgctgaa
    14041 cacctgctca atccgcgaga aggctcagga aaaagtcttc catcagttgg gtcgctggaa
    14101 actgttaaaa gagaagaatc cagacctgat tatcggcgtc ggcggctgcg tggcatcgca
    14161 agaaggcgag cacattcgcc agcgcgccca ctatgtcgat attatttttg ggccgcaaac
    14221 gctgcaccgc ctgccggaga tgatcaactc cgtgcgcggc gaccgcagcc cggttgtaga
    14281 tatcagcttc ccggaaatcg agaagtttga ccgtctgcca gaaccgcgcg ccgaagggcc
    14341 gaccgcgttt gtctccatca tggaaggctg caataaatat tgcacctact gcgtggtgcc
    14401 ttacacccgt ggtgaagagg taagccgtcc gtccgacgat attctgtttg agatcgccca
    14461 gcttgcggct cagggcgtgc gtgaagtcaa cctgctcggt cagaatgtga atgcctggcg
    14521 tggtgagaac tacgacggca ccaccggaac gtttgccgat ctgctgcgtc tggttgccgc
    14581 catcgacggg atcgatcgta ttcgctttac caccagccat ccgatcgaat tcaccgacga
    14641 tatcatcgaa gtgtatcgcg acacgccgga gctggtgagc ttcctgcacc tgccggtaca
    14701 gagcggttcc gatcgcattc tgaacctgat gggacgtacc catacggcgc tggagtacaa
    14761 agcgatcatc cgtaaactgc gtgcggcgcg cccggatatt cagatcagct ctgacttcat
    14821 cgtcggcttc cctggcgaaa ccaccgaaga cttcgagaaa acgatgaagc tgattgccga
    14881 cgtcaatttc gacatgagct acagctttat cttctctgca cgtccgggta caccagccgc
    14941 cgatatggtt gatgatgttc cggaagaaga gaagaagcag cgtctgtata ttctgcaaga
    15001 gcgcattaat cagcaagcga tggcatggag ccgccggatg ctcggcacca cccagcgcat
    15061 tctggtagaa gggacttcac gtaagagcat catggagctt tccggtcgca ctgaaaataa
    15121 ccgcgtggtt aacttcgagg gcacgccgga tatgatcggt aaattcgtcg atgtagaaat
    15181 taccgacgtc tacccgaact ctctgcgcgg taaagtggtg cgaactgaag atgaaatggg
    15241 tctgcgcgtg gcagaaacac cggaatcggt gattgcccgt acccgcaaag aaaacgacct
    15301 tggcgtgggt tattatcagc cgtaattctc aggcccgccg ttccggtggg ccttgttttt
    15361 tctctcttta tccccatctt tatcgcaatg cttgcgcctt tttcctgtgg cgtgaataat
    15421 ttccttatag gccggtgaat gttcagcttc cggcggcata cagataagag gaacggtttg
    15481 aacatagaca ctcgcgaaat caccctggag ccagcagaca atgcgcgtct gttgagcctg
    15541 tgcggcccgt ttgatgacaa catcaaacaa ctcgaacgcc gtctcggcat cgaaatcaat
    15601 cgccgcgata accactttaa actgaccggc cgtccgattt gcgtcaccgc tgcggcagac
    15661 attctgcgta gcctgtatgt cgatactgcc ccgatgcgcg gtcagattca ggatatcgaa
    15721 ccggaacaga tccaccttgc gattaaagaa gcgcgggtac tggagcaaag cgcggagagc
    15781 gtgccggagt acggcaaagc ggtcaatatc aagaccaaac gcggcgtaat taagccgcgc
    15841 acgccaaacc aggcgcagta catcgccaat attctcgacc atgacattac cttcggcgtt
    15901 ggcccggcgg gtacgggtaa aacctacctg gcagtggctg cggcagttga tgccctggag
    15961 cgtcaggaaa ttcgccgtat tctgctgact cgtccggcgg tcgaagccgg tgagaaactg
    16021 ggcttcctgc ctggcgattt aagccagaaa gtagacccgt atctgcgccc gctgtacgac
    16081 gcgctgtttg aaatgctggg ctttgagaaa gtcgagaaac tgattgagcg caacgttatt
    16141 gaagtcgcac cgctggccta tatgcgtggt cgtacgctga acgacgcgtt tatcattctt
    16201 gatgaaagcc agaacaccac catcgaacag atgaagatgt tcctgacccg tatcggtttt
    16261 aactcaaaag cggttatcac cggcgacgtc acacagatcg acctgccgcg taataccaag
    16321 tctggtttgc gtcatgccat cgaagtgctg gctgatgtcg aagagatcag ctttaacttc
    16381 ttccacagcg aagacgtggt tcgtcacccg gtggtggcgc gtatcgttaa cgcctatgaa
    16441 gcctgggaag aagccgaaca aaaacgtaaa gcggcgctgg cagcagaacg caagcgcgaa
    16501 gaacaggaac aaaaatgagt caggtgatcc tcgatttaca actggcatgt gaagataatt
    16561 ccgggttacc ggaagagagc cagtttcaga catggctgaa tgcggtgatc ccgcagtttc
    16621 aggaagaatc ggaagtgact attcgcgtgg tcgataccgc cgaaagccac agtctgaatc
    16681 tgacctatcg cggtaaggat aagccgacca acgtgctctc cttcccgttt gaagtaccgc
    16741 ctggcatgga aatgtcgcta ctgggcgatc tggttatctg ccgtcaggtg gttgagaagg
    16801 aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg gtgcacggca
    16861 gtctgcattt gttaggttac gatcacatcg aagatgacga agcagaagaa atggaagcca
    16921 tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc gagaaagaat
    16981 aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg acggcgtttt
    17041 gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac acagtagtga
    17101 cacgataagc aacaagaagg gatttttctc cctgttactc agccaacttt tccacggtga
    17161 accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga acgaccttat
    17221 cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc aacgcgttcg
    17281 cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga cgctggacga
    17341 atgccttgat gtcatcattg agtccgccca ctcacgtttc ccggtgatta gcgaagacaa
    17401 agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc gcagcgatgc
    17461 tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg aaagtaagcg
    17521 cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga tcgtcattga
    17581 cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac tgattgttgg
    17641 tgagattgaa gacgagtatg acgaagaaga tgatatcgac ttccgtcagc tgagtcgtca
    17701 tacctggacc gtgcgcgcac tggcttctat tgaagacttc aacgaagcgt tcggcaccca
    17761 ctttagcgat gaagaggttg acactatcgg tggtctggtg atgcaggcat ttgggcatct
    17821 tccggcgcgt ggcgaaacca tcgacatcga cggttaccag ttcaaagtgg cgatggccga
    17881 cagtcggcgt attattcagg ttcatgtcaa aatcccggat gactcacccc agccgaagct
    17941 ggatgaataa aaccgaaact ggatagataa ctacatggct tttgcctcat taattgaacg
    18001 ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc tggccttctc
    18061 tccttacgac gtctggcctg cggcgattat ttcgctgatg gggcttcagg cgttgacctt
    18121 taaccgccgt ccactccagt ctgccgctat tggcttttgc tggggatttg gcctctttgg
    18181 cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcggaatgc ctggcccggt
    18241 taacatcttc ctggtggtac tgctggcggc gtatttgtcg ctgtataccg gactgtttgc
    18301 tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg cgcgtggcga ttgccgcccc
    18361 tgctctctgg caagtgaccg agtttctgcg cggttgggta ctaacaggct tcccgtggtt
    18421 acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccga taatgggcgt
    18481 ggaagccatt aacttcctgc tgatgatggt tagtggcctg ctggcactgg cgttggtcaa
    18541 acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gcccttccct tcccgctgcg
    18601 ttacatccag tggtttaccc cgcaaccgga aaaaactatt caggtttcga tggttcaggg
    18661 cgatattccg caatcgctga aatgggacgg agaccagcta cttaatacgc tgaagattta
    18721 ctacaacgca acggcaccgc tgatgggcaa atcatcgttg attatctggc cggagtcggc
    18781 gataaccgat ctggaaatta atcagcaacc gttcctcaaa gctctggacg gtgagttgcg
    18841 cgataaaggt agctcgctgg tcaccgggat tgtcgacgcg cgtctcaata aacagaaccg
    18901 ctacgatacc tacaacacca tcatcacgct gggtaaaggt gcgccgtaca gctacgaatc
    18961 agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc cactggagtc
    19021 gatactgcgt ccgttagcac cgttctttga tttgccgatg tcgtcattca gccgcggacc
    19081 atatattcag ccaccgctgt cgttgaatgg cattcaactt actgcggcca tttgctacga
    19141 aatcattctc ggcgagcaag tgcgcgataa cttccgcccg gataccgact atctgctgac
    19201 tatctccaac gatgcgtggt ttggtaagtc tattggtcca tggcagcact tccagatggc
    19261 gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca acggcattac
    19321 ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttca cccgcgaggt
    19381 gttgaccact aacgtgacac caaccaccgg actcacaccg tacgcacgaa ccggcaactg
    19441 gccgctgtgg gtgctgacgg cgctgtttgg ttttgccgct gtactgatga gtctgcgtgc
    19501 tcgcaaacgt taatgtgcct gatgcgcgac gcttatcagg cctacgcgat ttttacaata
    19561 tgttgaattt tcgcgatttt gtaggataaa gcgtactttt tcaacacgct ctggggcatg
    19621 attatcatgc ccttttttct tacggttcag ggatacaagc cacatcgcca tattggggat
    19681 gccgaataat tgcctgcgga tcaagcgagc gcgtcctgtc catttggatt aacctgttct
    19741 tcgccataac aaatatcttt ctaccatccg ctgaaaaacg aagattatta acgtagctgg
    19801 cgggataaac gccaataatc tgcccggcat taatattgag caggatgaca taaccttgca
    19861 ggccatatga caccaacgct aaatactgac cgtccgggct aataccctca acggaataaa
    19921 cgctcctgct acaccattgc gtcataatgc tggcttgttg atcctcagct cttatacgtg
    19981 aaatcccctg gctatcggca aaaataatct caccattttt tggtcggaat cccatcgcag
    20041 aaacgacacc acgcgcatca cctgcgggaa agtcttcttt ctgatgcgtg atggtattga
    20101 tccgcacgat gttacccctg cctgctcccg gaaccagtaa tgttttttca tccggggaaa
    20161 gtgccataac ccaggcgcct ggtttaccgc tcatccccgt cccgtcgtat ttctccagcg
    20221 ggatatccgc cacatgctga taagccacca gatcaatcgc tgacacacta ttgctggtgc
    20281 gattcatcac ccaggctttt gtgccatcac gcgagaggat aaggctgtca ggatgggcgc
    20341 cggtggtaat cgactgaata atctcccccg tcgccacgtt aatgaccgat aacgcattcg
    20401 cttcaaatga agtcgcataa atcaactttt catccaggct taatgccagc gatctgacgc
    20461 cctgatcttt acagccacca caatgtttga ctgacttttc tttcgctatg ggcgctgtaa
    20521 aggtatgcag gattttcttt tgtccgaggt cgaaaacaac aatgctgttc gtgttttcaa
    20581 ttgcggcata caacgtatta ccatcccgac tgatcgccat atcatggata tcggcaatct
    20641 cagcgagtaa atcaccactg gcctgctcac gacttaccgg agcggataat gtttccctga
    20701 gggcgttgcg ctcctccact aactgctgct ttttttgtcg tcgctcttgt tcaatctcct
    20761 gcggataaaa cgcttcacgc aggctttgcc cgacctgtaa aactgattca tcaatctgct
    20821 gctgcacaaa ccagatagtc ccggtcagaa gaagagtgac cgcaacaata atcccgcctt
    20881 ccgtaacgag ccgattttgc ccaactaaac gcgcactcag ccaacccaac gcgccccccg
    20941 caatcaggcc gccaatgtgg caagcgttat ctattcctgt ttgccgggta ccatacaaca
    21001 gtgtcagcgc caccataccc aacagaggaa atactcgccg ctggttttta tggtgtgtgc
    21061 ccgcacctga agcaagctgt gttgctatcg cagcgcccgc tattcccata attgccccgg
    21121 atgccccaat gctgatgact atctgctcac tttcgcgtaa cgtataacag gcgctgatca
    21181 gcccaccaaa gatgccggag aataaccaga taatcaggag tcgccatttc cccagtatcc
    21241 gttccgccac tgtccccacc accaggagtg caaacatatt catcagcaaa tgggaaaaac
    21301 tactgtgcag aaaaacactg ctcaataagc gccaggattc atccgacaac gtgagagaag
    21361 caacgttagc gccgagatgg atgagtgcgg attgatcgac ttcttcgtaa ctcatccatt
    21421 gtgcgaggta acaaaatatc ccaacacaga gcgcaaaaag gatcagggtt attgcgggta
    21481 attgaacatt taacggctta accgaagatg cagacataag gtgattcttc cttgaaaaga
    21541 ctagtaaaaa gcattttccc gagtgtagtt atcatcccgg caaacgtccc acaccgaaaa
    21601 gttaacactg tccgaaatat gactttttca aactgaaatt acaatcgtaa ttaattcacc
    21661 cctaagtatt ttttggcatg taagttgctt tatttactgt gtaagcaatg aaatcagccg
    21721 caacgcaacc gggttgcacc accacaaatc agcggtagca ccaaaaaggt gcggtttaag
    21781 aattttgctt cggaatggtg cggcgcacct cgcaaaaata aacaataaaa caacaatatc
    21841 tttacattta tccaaactaa atgttaccta acaaacacaa cactgcacaa taaagtcgca
    21901 gatgataaca acacaaacac tcacaacggg tatccatgcg tttttaacgc agaagataaa
    21961 ggagttggat atgcaattac gtaaacctgc cacagcaatc ctggccctgg cgctttccgc
    22021 aggactggca caggcagatg acgccgcccc ggcagcaggc agcacgctgg acaaaatcgc
    22081 caaaaacggc gtgattgtcg tcggtcaccg tgaatcttca gtacctttct cttattacga
    22141 caatcagcaa aaagtggtgg gctactcgca ggattactcc aacgccattg ttgaagcagt
    22201 gaaaaagaaa ctcaacaaac cagacttaca ggtgaaattg attccgatca cctcacaaaa
    22261 ccgtattcca ctgctgcaaa acggcacttt cgattttgaa tgcggttcta ccaccaacaa
    22321 tgccgaacgc caaaaacagg cggctttctc tgacactatt ttcgtggtcg gtacgcgcct
    22381 gctgaccaaa aagggcggcg atatcaaaga ttttgccgac ctgaaaggca aagccgtagt
    22441 cgtcacttcc ggcaccacct ctgaagtttt gctcaacaaa ctgaatgaag agcaaaaaat
    22501 gaatatgcgc atcatcagcg ccaaagatca cggtgactct ttccgtaccc tggaaagcgg
    22561 tcgtgccgtt gcctttatga tggatgatgc tctgctggcc ggtgaacgtg cgaaagcgaa
    22621 gaaaccagac aactgggaaa tcgtcggcaa gccgcagtct caggaagcct atggttgcat
    22681 gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat gataccatcg ctcaggtgca
    22741 gacctccggt gaagcggaaa aatggtttga taagtggttc aaaaatccaa ttccgccgaa
    22801 aaacctgaac atgagtttcg aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa
    22861 tgacaaggca ctgaactaat tacaagaacc aggggcggaa attccagccc tctcgattgt
    22921 tacgtagcac ggacagacta tacgcctggt ggtcgttccc catcgggcct gaaaaccgca
    22981 atacgctggg taacaatctt cgagggtagc agttaacgct gctaccctct tttttctgga
    23041 gtagatttat gtctatagac tggaactggg gtattttttt acaacaagcc ccgttcggca
    23101 acaccaccta tcttggttgg atctggagtg gctttcaggt gacgatcgct ttgtcgatct
    23161 gcgcctggat tatcgctttc ctcgtcggtt catttttcgg cattttacgt accgttccaa
    23221 accgttttct ctccggtctg ggtacgttgt atgtcgaact gttccgcaac gtgccgttga
    23281 ttgtgcaatt ctttacctgg tacctggtga tcccggagct gctgccagag aaaatcggca
    23341 tgtggtttaa ggcggaactg gaccctaata ttcaattttt cctttcatcc atgctctgcc
    23401 tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg cgccgctatt cagtcgctgc
    23461 cgcgtgggca aaaaaatgcc gcgctggcga tgggtctgac gctaccgcag gcttaccgct
    23521 acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc gatgacctca gagatgatga
    23581 acctggtgaa aaactccgcc atcgcctcga ctatcggtct ggtggatatg gcggcgcagg
    23641 cgggtaaatt gctcgattac tcggcccacg cctgggagtc gtttaccgcc atcacgctgg
    23701 cgtatgtatt gattaacgct tttatcatgc tggtgatgac gctggttgaa cgtaaagttc
    23761 gtctgcccgg caacatgggg ggcaaataat gtacgagttt gactggagtt ccattgttcc
    23821 ttccctgcca tatctgctcg acgggctggt gatcacgctg aaaatcaccg tcacggcagt
    23881 ggtgattggt attttgtggg gcaccatgct ggcggtgatg cgcttgtcca gctttgcgcc
    23941 cgtagcgtgg tttgccaaag cctacgttaa cgtattccgc tcgattcctt tagttatggt
    24001 tttgctgtgg ttttacctga tcgtaccggg ttttctgcaa aacgtgctgg gattatcacc
    24061 caaaaatgat atccgtttaa tttcggcgat ggtggcgttt tcgatgtttg aagcggccta
    24121 ctattcagag attatccgtg ctggtattca aagtatttct cgtggtcagt cgagcgccgc
    24181 gctggcgttg ggaatgactc actggcagtc gatgaaactg attattctgc cgcaggcgtt
    24241 ccgcgcgatg gtgccgctgt tgctcactca gggcatcgta ctgtttcagg atacctcact
    24301 ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc tcaaccattg gtgagcgtga
    24361 tggtacgcag gttgagatga tcctgtttgc cggatttgtt tatttcgtta ttagccttag
    24421 cgcgtcgttg ttggtcagct acttgaaaag aaggacagca taatgattac cctgaaaaat
    24481 gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact gctcaaccga agtgaaaaaa
    24541 ggcgaagttg tggtggtttg cggtccgtct ggttccggca aatcaacgct gattaaaacc
    24601 gtcaacggcc tcgaaccggt gcagcaaggt gaaatcaccg tcgatggcat cgtggttaac
    24661 gacaagaaaa ccgatctggc gaaactgcgt tcccgcgtcg ggatggtgtt tcagcatttc
    24721 gagctgttcc cccatctgtc gattatcgaa aacctgaccc tggcgcaggt gaaagtgctt
    24781 aaacgcaata aagcgccggc tcgtgaaaaa gcattgaaat tgctggagcg cgtcgggctt
    24841 tccgcacatg ccaataagtt tccggcgcag ctttccggcg gccagcagca gcgtgtggcg
    24901 atcgctcgcg cattgtgcat ggatcctatt gccatgctgt tcgacgaacc aacgtcggcg
    24961 ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg tcgaactggc gaacgaagga
    25021 atgacgatga tggtggtgac ccacgaaatg ggctttgccc gtaaagtggc gaatcgggtg
    25081 atctttatgg acgagggtaa aattgtcgaa gactcgccga aagacgcttt cttcgatgat
    25141 ccgaaatcgg accgcgcaaa agacttcctc gcaaaaatcc tgcattaatc actctggcgc
    25201 gcatactcaa ttgcgcgcca tctcacgctt tctcgtttcg tccctcgtat ctgttacatc
    25261 ccggcgttac ccttgtcgca aagaaacaca caacaaggag caacaatggc actgccaatt
    25321 ctgttagatt gcgacccagg tcatgacgac gctatcgcaa tcgttctcgc cctcgcctca
    25381 ccagagcttg atgtcaaagc aattacgtct tccgccggaa accagacacc agaaaaaacc
    25441 ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca ccgatattcc ggtagcaagc
    25501 ggtgcggtaa aaccgttaat gcgtaatttg attattgcgg acaacgtgca cggcgaaagc
    25561 ggtctcgacg gcccggcatt accggaaccg acgttcgcac cgcaaaactg cacggcagtg
    25621 gagctgatgg cgaaaacgct gcgtgaaagt gaggaacctg tcaccattgt ttctaccgga
    25681 ccgcaaacta acgttgcctt gctgctcaat agccacccgg aactgcatag caaaattgcc
    25741 cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact ggacgcctgc ggctgaattt
    25801 aatatttacg ttgacccgga agcggcagaa attgtcttcc agtcaggaat tccggtggtg
    25861 atggccggtc tggatgttac tcacaaagca caaatccacg ttgaagacac tgagcgtttc
    25921 cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac tgctggattt cttcctcgaa
    25981 tatcacaaag acgaaaaatg gggctttgtc ggcgcaccac tgcatgaccc atgcaccatc
    26041 gcctggctgt tgaaaccgga gttgtttacc actgttgagc gctgggttgg cgtggaaaca
    26101 caagggaaat atacccaggg tatgacggtt gttgattatt attatctgac tggcaataaa
    26161 ccgaatgcca ccgtaatggt cgatgttgat cgtcagggct ttgttgattt actggctgat
    26221 cgtctgaaat tttacgctta atcactcgcc ggatgacatc acgccatccg gcaccactca
    26281 tttcattacg gctcgaacgg gcgtctctgg aactgatcat gaccacattt cgggcatagc
    26341 gtcagcactt ccggtgtata gatcgggaga tggaagtgac atttctcgca gaccagattt
    26401 cccagcccta ccacttctcc gctgtgataa accccgtgat gattgaggtc ctggaaaact
    26461 tcgcgccatt caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct
    26521 ttaatcaccc gcataaagac gctgtcagac tcttctttca ggctctcttc atagctcatg
    26581 gcgaactctt ccaggtcacg tctgacagct cgcgtcagct catcgacctc ggttcgcgtt
    26641 aactcccctg tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt
    26701 tcgccattgc gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc
    26761 ttgttcatcg tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc
    26821 cttgctttgc taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca
    26881 tttcaacgtc gagtgcgcga aaggctgttt tggcgcgggc gtttgggcta tgctatgcgg
    26941 atctgaaaaa ccacatcaac gctacatttg tagcagtatt gaaaacagga ccactggctg
    27001 ccatgcaaga gcaataccgc ccggaagaga tagaatccaa agtacagctt cactgggatg
    27061 agaagcgcac atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta
    27121 tgcttcccta tccttctggt cgactacaca tgggccacgt tcgcaactac accatcggtg
    27181 acgtgatcgc ccgctaccag cgcatgctgg gcaaaaacgt cctgcagcca atcggctggg
    27241 acgcatttgg tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgcg ccagcaccgt
    27301 ggacgtacga caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg
    27361 actggagccg cgagctggcg acctgtacgc cggaatacta ccgttgggaa cagaaattct
    27421 tcaccgaact gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc
    27481 cgaacgacca gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg
    27541 ataccaaagt tgagcgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg
    27601 acgagctgct caacgatctg gataaactgg atcactggcc agacaccgtt aaaacaatgc
    27661 agcgtaactg gatcggtcgt tccgaaggcg tggaaatcac cttcaacgtt aacgactatg
    27721 acaacacgct gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg
    27781 cggtggctgc gggtcatccg ctggcacaga aagcggcgga aaataatcct gaactggccg
    27841 cctttattga cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga
    27901 aaaaaggcgt cgatactggc tttaaagcgg ttcacccatt aacgggcgaa gaaattcccg
    27961 tttgggcagc aaacttcgta ttgatggaat acggcacggg cgcagttatg gcagttccgg
    28021 gtcacgacca gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccggtta
    28081 tcctggcagc tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg
    28141 tgctgttcaa ctctggcgag tttaatggtc ttgaccatga agcggccttc aacgccatcg
    28201 ccgataaact gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact
    28261 ggggtgtttc tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg
    28321 gtaccgtaat gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga
    28381 tggacggcat taccagcccg attaaagcag atccggaatg ggcaaaaact accgttaacg
    28441 gtatgccagc actgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact
    28501 atgcgcgcta cacttgcccg gagtacaaag aaggtatgct ggattccaaa gcggctaact
    28561 actggctgcc ggtggatatt tacattggtg gtatcgaaca cgccattatg cacctgctct
    28621 acttccgctt cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag
    28681 cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggtgaaa
    28741 acggcgaacg taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc
    28801 gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa
    28861 tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg
    28921 acaccgttcg tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg
    28981 aatccggcgt ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtgtacgagc
    29041 acacagcaaa aggtgatgtt gcggcactga acgtcgatgc gctgactgaa gatcagaaag
    29101 cgctgcgtcg cgatgtgcat aaaactatcg ctaaagtaac cgatgatatc ggccgtcgtc
    29161 agaccttcaa caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagccc
    29221 cgaccgatgg cgagcaggat cgcgccctga tgcaggaagc gctgctggcg gtggtccgta
    29281 tgcttaaccc gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag
    29341 gcgatatcga caacgcgccg tggccgattg ctgacgaaaa agcgatggtg gaagactcca
    29401 cgctggtcgt ggtgcaggtt aacggtaaag tccgtgcaaa aatcaccgtt ccggtggacg
    29461 caacggaaga acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg
    29521 atggcgttac tgtacgtaaa gtgatttacg tacccggtaa actcctcaat ctggtcgttg
    29581 gctaagcgcg ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt
    29641 aatcaccgcc gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa
    29701 ggtcatgatc ctggactcag gcgatccgaa cgggccatta agccgtgcgg tgcgtaacca
    29761 gttacgtctg aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc
    29821 cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca
    29881 aacagcagag tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga
    29941 tatctacccg attagcgcca aagtcttccg ttcgttcttc gataacccgc aaatggcgtt
    30001 agcgaaagat aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca
    30061 gctgattcgt aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac
    30121 gtcgaccaca acggatactc tggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg
    30181 atgattcggt tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg
    30241 tatcttttac ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag
    30301 gtcgctgcgg cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac
    30361 tggaatgcga tcttttcgtt atgccaggct atgagtctgt ttgccagtag acaaacgcta
    30421 ttgctgttgt taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc
    30481 accggacttc tccatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg
    30541 caagaaaatg ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag
    30601 acaccggagc aggctcagct tccccgctgg gttgctgcgc gcgcaaaaca gctcaactta
    30661 gaactggatg acgctgcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg
    30721 ctggcccagg cactggagcg tttatcgcta ctctggccgg acggcaaact gacattaccg
    30781 cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct
    30841 ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc agcagctgcg tctggaaggc
    30901 agcgaaccgg ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg
    30961 aaacgccagt ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag
    31021 aaccgcaggg gcatgatgag cgaggcgtta aatcgcttaa gccagccgca gttacgtcag
    31081 gccgtgcaac tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg
    31141 tgggcagagc tggaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt
    31201 atcgacggtt gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca
    31261 ctatggtcat cttaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac
    31321 aatcatccct aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgcgcagcg
    31381 taaacacatg cttgaactgg cgatagctga caagccactg tttacccttg atgaacgcga
    31441 gctaaagcgc aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggagca
    31501 aggaccggac gtgccgctgg cgtttattat tggtcaggat tcattgctga cctttccgac
    31561 ctggtacgaa tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtcctgg
    31621 ttacccactt gaaatggcgc aaccgcaata ccagcaatgg ctggaggatc atttgacaca
    31681 taacccggaa gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg
    31741 gtttaacatc tcggcgacca tcatccgcga acgtttgcaa aatggtgaat cgtgtgagga
    31801 tttattaccg gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct
    31861 gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat
    31921 gtcgatggtc tgtacagcgg tcatgcgccc acacccctga ccgcgcgcgg tattgagcaa
    31981 gcgcaaaatc tgcatacgct gctacatgat gtttcctttg acctggtttt atgcagtgaa
    32041 ctggaacggg cacagcatac cgcgcgactg gttcttagtg accgccagca ccccgttcat
    32101 atcatccctg aactcaatga aatgtttttt ggcgactggg agatgcgaca tcatcgtgac
    32161 ctcatgcaag aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc
    32221 cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg tggaacgatt tatcgcaagg
    32281 cttagtgaat atcagcacta tcagaatatt ttagtcgtca gccatcaggg cgtactgagt
    32341 ctgttaattg ctcgtttaat tggcatgcct gccgagtcta tgtggcattt ccgcgttgac
    32401 caggggtgtt ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat
    32461 agccgtgcca tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa
    32521 cgaccattga cagccccggg caggctgata ttctccgcag ccagactttt tccgccagac
    32581 acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat
    32641 gatagcccac tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag
    32701 gtatactgac agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa
    32761 agcactccag gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc
    32821 cttagacgtt cagggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc
    32881 cagccgccat gttatgtcca ttgctgatca cgttgtgcag gagtctcgcg cagcgggcct
    32941 gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga
    33001 tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg
    33061 gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca
    33121 aaccggtttt accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga
    33181 aattccggcc ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg
    33241 tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa
    33301 gccctgggat acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga
    33361 cgtcagtcta ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga
    33421 gcagagctgg tcgctgtcgg cacttaccct tccccatccg ctggttcgcg tgctggtcgc
    33481 agagagtctg taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata
    33541 agtgagcttt gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac
    33601 ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct
    33661 gaccggcgtg cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca
    33721 gacccgctct aatgaaaacc gcattaagct ggtgcctatc gcacccagcc gcggcattat
    33781 ctacgaccgt aacggtatcc ctctggctct caaccgcact atctaccaga tagaaatgat
    33841 gccggagaaa gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct
    33901 gaccgatgat gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc
    33961 tattccggtg aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaaccagta
    34021 ccgttttccg ggtgttgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc
    34081 gttgacccac gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa
    34141 taatgacggc aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga
    34201 acgttactat gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa
    34261 ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta
    34321 cctgacgctg gatctcaaac tccagcaata tattgaaacg ttgctggcgg gtagccgcgc
    34381 agctgtggta gtcaccgatc cgcgtacagg tggggtgttg gcgctggttt ccacgcctag
    34441 ttatgaccca aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgctgaa
    34501 cgacccgaat acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac
    34561 agttaaaccc tatgttgcgg tttcggcatt gagtgccggg gtgatcacgc gcaatacgac
    34621 gctgtttgac cccggctggt ggcaactgcc agggtcggaa aaacgttatc gtgactggaa
    34681 aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt
    34741 cttctatcag gtggcctacg atatggggat cgatcgcctc tccgaatgga tggggaaatt
    34801 cggttatggt cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac
    34861 ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt
    34921 tggtatcggt cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat
    34981 cctgattaat gacggtatcg tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg
    35041 caaacaggtg ccatgggtac aaccgcatga accgcccgtc ggcgatattc attccggtta
    35101 ctgggagctg gcaaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca
    35161 taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa tctggtacag ctcaggtctt
    35221 cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca
    35281 caaactgatg accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct
    35341 ggagaacggc ggcgcgggtc cggcggttgg tacgctgatg cgccagatcc tcgaccacat
    35401 tatgctgggt gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga
    35461 ggaccattaa tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc
    35521 gatcccacaa tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg
    35581 agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa tcggccagat tgcgatgggt
    35641 ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc
    35701 tatctctata tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct
    35761 aaaggcgctc aacgctggct ggacctcggt attgttcgtt ttcaaccgtc ggaaattgcc
    35821 aagatagccg taccgttgat ggttgcacgc tttatcaacc gcgacgtttg cccaccttca
    35881 ttgaagaata cagctatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca
    35941 cagcctgact tgggaacatc aatcctcgtt gcgctttccg gcctgtttgt actgttcctc
    36001 tctggcctta gctggcgtct gattggcgtc gcagtagtgc tggtagcggc gttcattccg
    36061 attctgtggt tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac
    36121 ccggaatcag acccactcgg cgcaggctat cacattattc agtctaaaat tgctattggc
    36181 tccggcggat tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc
    36241 cccgaacgcc ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc
    36301 attttgattc tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc
    36361 agagcgcaaa ccacctttgg tcgcgtcatg gctggcggct taatgctgat attattcgtt
    36421 tatgtcttcg taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc
    36481 ccactggtca gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta
    36541 atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg
    36601 taagcagtgg ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga
    36661 tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga
    36721 aattagcggg gcagacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca
    36781 gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact
    36841 ggcggcaatc tatgatgccg aacccggcag taacctgacg gcctctggcg aagctttcga
    36901 tccgacacag ttgacggcgg cccatccaac gcttccaatc cccagctacg ccagaatcac
    36961 taacctggct aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttatggcaa
    37021 cgaccgcgtg atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac
    37081 caaagttcgt atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat
    37141 ggcttgtacc acagtcgcca aacagactta cgccctgcca gcacctcccg atttaagcgg
    37201 tggcgcggga acaagttcag tgcctgtccc gcagggtgac attcttccgg tcagtaattc
    37261 gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc agcagcggtt tcctcggcgc
    37321 accaacgacc ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc
    37381 cgttgttaca gctccgtcga caacgcctgc aacctcacct gcaatggtga taccgcaagc
    37441 cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc ggggccgtta gcgatcaggc
    37501 tcgtgcgcaa cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac
    37561 tcaaaatggc gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag
    37621 taccttgcag caacgtttgc aaaccgaagc ccaattacag tcatttatta ccaccgcgca
    37681 gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc
    37741 ggaaagtcag atgcctgccg gtagtgcatt tgctatagta gggcactttt tttaattcca
    37801 tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc
    37861 tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga
    37921 atatcaaaac catgatcccg ggtgtaccgc agatcgacgc ggagtcctac atcctgattg
    37981 actacaactc cggcaaagta ctcgccgaac agaacgctga tgtccgccgc gatcctgcca
    38041 gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta
    38101 aagaaactga tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta
    38161 aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc
    38221 gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg
    38281 gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgccctg ggcctgaaaa
    38341 atacccactt ccagacggta cacggtctgg atgctgatgg tcagtacagc tccgcgcgag
    38401 atatggcgct gatcggccag gcgttgatcc gtgacgtacc gaatgaatac tcgatctata
    38461 aagaaaaaga atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg
    38521 ataacagcct gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc
    38581 ttgttgcttc tgcgactgaa ggccagatgc gcttgatctc tgcggtgatg ggcggacgta
    38641 cttttaaagg ccgtgaagcc gaaagtaaaa aattgctaac ctggggcttc cgtttcttcg
    38701 aaaccgttaa cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg
    38761 attctgatcg cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccgcgtggtc
    38821 gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc
    38881 aaaagaatca ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgaacaac
    38941 gcccgctggt tgtgctgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt
    39001 acattaaatt aatgttccat cactggtttg gttaaaaatt gagcacttga aagtgtaatt
    39061 tccgtcccca tatactaagc atcagtaaaa aactcccgcc ttctggcggg agttgctatt
    39121 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    39181 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    39241 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    39301 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    39361 ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    39421 ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    39481 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
    39541 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
    39601 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaagggca
    39661 ggcaggaaag gcggagcata ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    39721 cggtgggcag gtgacttatc acgggccggg gcaacaggtg atgtatgtgt tgcttaacct
    39781 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    39841 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
    39901 cggggaaaag aaaatttgct cactgggttt acgtattcga cgcggttgtt cattccacgg
    39961 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    40021 tgcaggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    40081 tgcaccacgt ttactggaaa atattttagc gctactaaac aatcctgact tcgaatatat
    40141 taccgcttaa ttccatacat caatggccca atttacactg ggtcattact cataatacct
    40201 gctaatgctt tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcga
    40261 tgctacgcct tgctccgtag ctcacccaat tggttaactc attgaaataa ttaccatggt
    40321 gagcaaaagc gttccgtccc aattagac
//
LOCUS       DAZFRG010000012        40158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFRG010000012 DAZFRG010000000
VERSION     DAZFRG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.012"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..372
                     /gene="yjbE"
                     /locus_tag="ACHKUC_000465"
     CDS             130..372
                     /gene="yjbE"
                     /locus_tag="ACHKUC_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO6616782.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            487..1125
                     /gene="yjbF"
                     /locus_tag="ACHKUC_000466"
     CDS             487..1125
                     /gene="yjbF"
                     /locus_tag="ACHKUC_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO6616783.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1122..1859
                     /gene="yjbG"
                     /locus_tag="ACHKUC_000467"
     CDS             1122..1859
                     /gene="yjbG"
                     /locus_tag="ACHKUC_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6616784.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVMLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1859..3955
                     /gene="yjbH"
                     /locus_tag="ACHKUC_000468"
     CDS             1859..3955
                     /gene="yjbH"
                     /locus_tag="ACHKUC_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6616785.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLVQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4002..4280)
                     /locus_tag="ACHKUC_000469"
     CDS             complement(4002..4280)
                     /locus_tag="ACHKUC_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616786.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4495..4905
                     /gene="psiE"
                     /locus_tag="ACHKUC_000470"
     CDS             4495..4905
                     /gene="psiE"
                     /locus_tag="ACHKUC_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO6616787.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4999..5889)
                     /gene="malG"
                     /locus_tag="ACHKUC_000471"
     CDS             complement(4999..5889)
                     /gene="malG"
                     /locus_tag="ACHKUC_000471"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO6616788.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5904..7448)
                     /gene="malF"
                     /locus_tag="ACHKUC_000472"
     CDS             complement(5904..7448)
                     /gene="malF"
                     /locus_tag="ACHKUC_000472"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO6616789.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7602..8792)
                     /gene="malE"
                     /locus_tag="ACHKUC_000473"
     CDS             complement(7602..8792)
                     /gene="malE"
                     /locus_tag="ACHKUC_000473"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO6616790.1"
                     /translation="MKIKTGARILALSALTTMMFSASTLAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9157..10272
                     /gene="malK"
                     /locus_tag="ACHKUC_000474"
     CDS             9157..10272
                     /gene="malK"
                     /locus_tag="ACHKUC_000474"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO6616791.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10344..11684
                     /gene="lamB"
                     /locus_tag="ACHKUC_000475"
     CDS             10344..11684
                     /gene="lamB"
                     /locus_tag="ACHKUC_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO6616792.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            12098..13018
                     /gene="malM"
                     /locus_tag="ACHKUC_000476"
     CDS             12098..13018
                     /gene="malM"
                     /locus_tag="ACHKUC_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO6616793.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13197..13694
                     /gene="ubiC"
                     /locus_tag="ACHKUC_000477"
     CDS             13197..13694
                     /gene="ubiC"
                     /locus_tag="ACHKUC_000477"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO6616794.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13707..14579
                     /gene="ubiA"
                     /locus_tag="ACHKUC_000478"
     CDS             13707..14579
                     /gene="ubiA"
                     /locus_tag="ACHKUC_000478"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO6616795.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14734..17157)
                     /gene="plsB"
                     /locus_tag="ACHKUC_000479"
     CDS             complement(14734..17157)
                     /gene="plsB"
                     /locus_tag="ACHKUC_000479"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO6616796.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17328..17696
                     /gene="dgkA"
                     /locus_tag="ACHKUC_000480"
     CDS             17328..17696
                     /gene="dgkA"
                     /locus_tag="ACHKUC_000480"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6616797.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17806..18414
                     /gene="lexA"
                     /locus_tag="ACHKUC_000481"
     CDS             17806..18414
                     /gene="lexA"
                     /locus_tag="ACHKUC_000481"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO6616798.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18487..19812
                     /gene="dinF"
                     /locus_tag="ACHKUC_000482"
     CDS             18487..19812
                     /gene="dinF"
                     /locus_tag="ACHKUC_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO6616799.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            19928..20137
                     /gene="yjbJ"
                     /locus_tag="ACHKUC_000483"
     CDS             19928..20137
                     /gene="yjbJ"
                     /locus_tag="ACHKUC_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO6616800.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20179..20694)
                     /gene="zur"
                     /locus_tag="ACHKUC_000484"
     CDS             complement(20179..20694)
                     /gene="zur"
                     /locus_tag="ACHKUC_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO6616801.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20850..21845
                     /gene="dusA"
                     /locus_tag="ACHKUC_000485"
     CDS             20850..21845
                     /gene="dusA"
                     /locus_tag="ACHKUC_000485"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO6616802.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21979..22221
                     /gene="pspG"
                     /locus_tag="ACHKUC_000486"
     CDS             21979..22221
                     /gene="pspG"
                     /locus_tag="ACHKUC_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO6616803.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(22387..23370)
                     /gene="qorA"
                     /locus_tag="ACHKUC_000487"
     CDS             complement(22387..23370)
                     /gene="qorA"
                     /locus_tag="ACHKUC_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO6616804.1"
                     /translation="MAIRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23453..24868
                     /gene="dnaB"
                     /locus_tag="ACHKUC_000488"
     CDS             23453..24868
                     /gene="dnaB"
                     /locus_tag="ACHKUC_000488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6616805.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24921..26000
                     /gene="alr"
                     /locus_tag="ACHKUC_000489"
     CDS             24921..26000
                     /gene="alr"
                     /locus_tag="ACHKUC_000489"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO6616806.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26253..27446
                     /gene="tyrB"
                     /locus_tag="ACHKUC_000490"
     CDS             26253..27446
                     /gene="tyrB"
                     /locus_tag="ACHKUC_000490"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO6616807.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSSAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            27628..30372
                     /locus_tag="ACHKUC_000491"
     CDS             27628..30372
                     /locus_tag="ACHKUC_000491"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6616808.1"
                     /translation="MENMTSPGTLLSGDNATWLEDYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAIIQLINAWRTQGHLRAKLDPLGLNPPADVPSLLPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNAPQADVQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKCLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPFKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLTLRQDMAAGT
                     QPIDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELSEFTDGAYKPVVTDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQRECEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            30405..31559
                     /gene="odhB"
                     /locus_tag="ACHKUC_000492"
     CDS             30405..31559
                     /gene="odhB"
                     /locus_tag="ACHKUC_000492"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6616809.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            31571..32989
                     /gene="lpdA"
                     /locus_tag="ACHKUC_000493"
     CDS             31571..32989
                     /gene="lpdA"
                     /locus_tag="ACHKUC_000493"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6616810.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            33011..34180
                     /gene="sucC"
                     /locus_tag="ACHKUC_000494"
     CDS             33011..34180
                     /gene="sucC"
                     /locus_tag="ACHKUC_000494"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6616811.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFATFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            34193..35065
                     /gene="sucD"
                     /locus_tag="ACHKUC_000495"
     CDS             34193..35065
                     /gene="sucD"
                     /locus_tag="ACHKUC_000495"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6616812.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHEMNKPVVGYIAGVTAPKGKRMGHVGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            35277..36776
                     /locus_tag="ACHKUC_000496"
     CDS             35277..36776
                     /locus_tag="ACHKUC_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFO6616813.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSAILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            36788..37861
                     /locus_tag="ACHKUC_000497"
     CDS             36788..37861
                     /locus_tag="ACHKUC_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO6616814.1"
                     /translation="MSLQITVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGHMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(37850..39208)
                     /locus_tag="ACHKUC_000498"
     CDS             complement(37850..39208)
                     /locus_tag="ACHKUC_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFO6616815.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(39201..>40158)
                     /locus_tag="ACHKUC_000499"
     CDS             complement(39201..>40158)
                     /locus_tag="ACHKUC_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6616816.1"
                     /translation="MVPLDNLYWSWAILLVITLIIYLLFLLFIKYWRMRSHAQQLLTL
                     ANETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMAT
                     EIAHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFAS
                     RHRVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNL
                     ISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGM
                     GLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
BASE COUNT         9487 a         9994 c        10816 g         9861 t
ORIGIN      
        1 attccaatta tcctaaaact ccatcactaa ttccccgcgc cgtaattcgc atgctttagt
       61 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
      121 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
      181 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag gatcggcagc aacgtcggtt
      241 tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
      301 ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac cacgaccacc
      361 agtacccagt aataaagtat gtatccccaa aataattcga gtcatcgcat ctgaagcttg
      421 aagtatgaag gggattaacc ataaccacac tccggtgtgg ttattctgcc cctctggaga
      481 agagtcgtga agcgaccagc actcattctt atctgcctgc tattacaggc ctgttcagcc
      541 acgactaaag agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag
      601 ctgacggacg atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc
      661 gggccgcagt tatttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact
      721 caggatcagg ccacccttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc
      781 gacaacctga ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt
      841 gttgatggtg caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac
      901 gccaccgcac gctcagtctt caaatgggat ggcaccgata ccgtcaaagt cggcagcgat
      961 gaaactccgg ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac
     1021 cgctactgga tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat
     1081 tatttcccgg tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg
     1141 tcgcattgat tttgagtgtg ggggcgtcat cggtcttcgc ggcgggaacc gtcaaggtat
     1201 tcagcaatgg cagcagtgag gccaaaacgc tgacgggtgc agagcattta atcgatctgg
     1261 taggccaacc acggctggca aacagttggt ggcccggtgc ggtgattagc gaagagctgg
     1321 caacggcggc agcattgcgt cagcagcagg cgttgctgac acggctggca gaactggctg
     1381 cagattccag cgccgacgat gccgccgcga ttaacgcctt acgccagcaa attcaggcgt
     1441 tgaaagtgac gggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac
     1501 gcggtaaccc gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg
     1561 tcatgttgtt cgggcttatc agccgtcctg gcaagcagcc attcactcca ggtcgcgacg
     1621 tggcgagcta tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg
     1681 tggtttaccc tgacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg
     1741 tagagccgat gcccggcagc attatttatg ttggcctcgc ggactccgtc tggagtgaga
     1801 cacctgatgc ccttaacgcc gacattcttc agactctgac gcagcggata ccccaataat
     1861 gaaaaaaaga catctgctta gcttactggc gctgggtatt agcacagctt gctacggcga
     1921 aacatatcct gcgcccattg gtccgtcgca atcggatttc ggtggcgtag gattattaca
     1981 aacgcccacc gcgcgcatgg cgcgggaagg ggagctgagc ctgaactatc gcgataatga
     2041 tcagtaccgt tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg
     2101 ctacaccgac gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac
     2161 gtataaagat aaagccttcg atctcaaact gcgtttgtgg gaagagagtt attggctgcc
     2221 gcaagtggcg gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct
     2281 tgttgccagc aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatatct
     2341 gggcaccagc ggtaatgtga aaaatccgct ctgttcagcc agtgataaat attgctatcg
     2401 cgataacagc tacaaacaag cgggatctat cgacggcagt cagatgttcc acggcccggc
     2461 ctcactgttt ggcggcgtgg aataccagac gccctggcaa ccgctgcgcc tgaaactgga
     2521 gtatgaaggt aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt
     2581 taacgtcggg gcgatttatc gcgttaccga ttgggccgac gttaacctta gctatgaacg
     2641 tggcaacacc tttatgtttg gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc
     2701 ctacaacgat aacgcccgac cgaaatacca gccgcaaccg caggatgcca ttttgcagca
     2761 ctcggtggtg gcgaatcagt taacgctgtt gaaatacaat gccggactgg ccgatccgca
     2821 gatccaggcg aaaggcgata cgctgtatgt caccggcgag caggtgaaat atcgtgattc
     2881 gcgcgaaggg atcatccgtg ctaatcggat cgtgatgaac gatctgcctg atgggatcaa
     2941 aacgatccgc attacggaaa atcgccttaa catgccgcag gtgacgacgg aaaccgatgt
     3001 cgccagcctg aaaaatcatc tcgccggaga gccgttgggc cacgaaacga agctggtgca
     3061 aaaacgcgtc gagccagtgg ttccgaagtc caccgagcag ggctggtata tcgacaaatc
     3121 acgctttgat ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt
     3181 ttacatgtat cagctgggcg tgatgggaac ggcggatttg tggctgacgg accatctgct
     3241 gaccaccggc agcctgtttg ctaaccttgc caacaactac gacaagttta actacaccaa
     3301 tccaccgcag gactcgcact taccgcgcgt gcgtacccat gtgcgcgagt atgtgcagaa
     3361 tgatgtctat gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta
     3421 cggtcaggtc tacggaggtt atctcgaaac catgtttggc ggcgtggggg cagaagtgtt
     3481 gtatcgcccg ctggatagca actgggcgtt tggtctggat gccaactacg ttaaacagcg
     3541 cgactggcgt agcgcaaaag atatgatgaa attcaccgat tacagcgtga aaaccggaca
     3601 tctgaccgcg tactggacgc catctttcgc ccaggacgtg ttagtgaaag cgagcgtcgg
     3661 gcagtatctg gctggggata aaggcggcac gctggagatc gccaaacgct ttgatagcgg
     3721 cgtggtagtg gggggctatg ccacgatcac gaatgtttcg aaagaggagt acggcgaagg
     3781 ggacttcacc aaaggcgtgt atgtctctgt accgctggat ctcttctcgt ctggcccgac
     3841 acgcagccgt gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg
     3901 gcgtaagttc cagttgtatg atatgaccag cgaccgtagc gtcaatttcc gctaagtcat
     3961 gggaaaggtg ccagttttcg cactcactgg ccccttcatt cttaatggcc tgtcgagcca
     4021 tcaggaacgc agttcacccc cggtttgcga tctggtgtgt aaaggcgaaa acattctgtc
     4081 ttcctgtccg tcgatatcgg ttgcaggtct ggttgcctga gcgactgggc catcattggg
     4141 ctggagacat tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa
     4201 gcaggaagaa cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc
     4261 tttatcaggt tacgtttcat ttgttccaga ggaacattgt cgatttttcg cgcattgctg
     4321 gtggctggga atcacctgaa tgggtgattt ttttgggctg tgtgttttgc cagatgcgac
     4381 attgatggga gatgcccact taattgagct gaacaaaaca tacacaaaaa atatagatct
     4441 ccgtcacatt tttgcgttat acaggaagct cgccactgtg aaggaggtac tgctatgacg
     4501 tcactctctc gtccgcgcgt ggagtttata tccaccattt tgcagaccgt actcaatctt
     4561 ggcctgttgt gcctcggcct gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg
     4621 gctgatgtgc tgttcgcgcc agaacaaacc agtaaatatg agctggtaga agggctggtg
     4681 gtttactttc tctatttcga atttatcgcg ctgattgtga agtactttca gtccggtttt
     4741 cacttcccgc tacgttactt tgtctatatt gggatcaccg ccattgtgcg cttgatcatc
     4801 gtcgatcata aatcgccact ggatgtgttg atctactcgg cagcgatcct gttactggtg
     4861 atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg agtaaaaaaa ggcgctccgt
     4921 ggagcgccga ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     4981 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     5041 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     5101 aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc tgcatcccca cagccagggt
     5161 gtagctgttt acgtcacgca gtaatagcga cgcgaccgga acttcagtaa tggcagcgat
     5221 aaacgacagg atgaacacaa ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     5281 gaaggcctgc cacggtgtcg caccatccag cgccgccgct tcttccagcg aactgtcgat
     5341 ggtttcgaaa taacctttga tggtccagac atgcagcgca atcccgccca gatacgcgaa
     5401 aattacgccg ccgtgagtat tcaggccaat gaatggaatg tactcaccca aacgatcaaa
     5461 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     5521 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     5581 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     5641 cagcagtacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cactaaaacc
     5701 taacgccagt ttccagtgat cccaggagat ttgctccggg atcagactac cggtcgcgaa
     5761 gttcccctga cgcagcgaga tggcgacgac catcagcagc gggaacataa tcgccgcgat
     5821 aaaaagtagc agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac
     5881 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     5941 atcgccagcg cgcccaccag caggaagatc agcgtggcaa ttgctgctgc cagaccgaag
     6001 tcctgacccc cgccgccttc aaaagcgata cggtaggtgt agttaacaag caggtcggta
     6061 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc
     6121 agcacgaagt tgttaaagtt aaaggcaaag ctggcgatca tcagcggtgt cagcggttta
     6181 atcagcagcg gcagcgtaat cttaaagaag ttctggaatg gacctgcgcc atccattgct
     6241 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     6301 tacggataac ccagccaggt gttgacgata atcagcatcg tgcgggcggt ggtcggatcg
     6361 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ttcaccgaaa
     6421 ctctggttaa acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     6481 agaataagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac
     6541 gccagaacca tgccgaccgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     6601 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtaaagac gcgggtaaag
     6661 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcccc ccagttgcca
     6721 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     6781 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggttgcgt gccagaaaac
     6841 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     6901 agtgcctgac gattctgggt aatcacgcgc agattcgcgc gctcgccttc aggctgcgtg
     6961 gcgctttctt tcaattgcag tttttgctcg ccgccaaatt tgaaagcgtc ggagaggtaa
     7021 tttttgccgg tttcgccgtc gctgagcgcc agttgccact catcgcccgc cggataaaga
     7081 ccaaaattat agattttgcc tgcttgccag gagcgatcta gcaacacttc ctgcgcacgt
     7141 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     7201 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     7261 taggctttac gattggcgaa aatatacagc cccgctgagc tcaatatcag cgtggtaatg
     7321 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     7381 agcagaccta acactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     7441 acatccatgg ggttcttcct cattccagga cggataaggc tttcatacct tatccgacaa
     7501 caactgcctg atgcgacgct gacgcgtctt atcaggtcta catacgtttc aatttcgtag
     7561 gccggataag gcgtttacgc cgcatccggc atttcacagc attacttggt gatacgagtc
     7621 tgtgcatctt tcagcgcagc atccacagtc tgacgaccgc tggcggcgtt gatcaccgca
     7681 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcataat ttcacctttc
     7741 tgagcgttat ccatggtggc ggcaatacgt ggatctttcg caagttcatc ctgataggac
     7801 ttcagcgcta ctgcacccag cggtttgtct ttattgaccg cttccagacc ttcgtcagtc
     7861 agcagatagt tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata
     7921 cctgcgctca gcacgccaac gaacggttta gatggttgac ctttgaaggt cggcagtacc
     7981 gttacaccat aattcacttt actggtgtcg atgttggacc atgcccacgg gccgttgata
     8041 gtcatcgctg tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca
     8101 ttcatgtgtt tgtttttaat cagatcaacc aggaaggtca gacccgcttt cgcgccagcg
     8161 ttatccacgc ctacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc
     8221 ccgtcagccg caattagcgg ccaggtgaag tatggttctt gcaggttgaa catcagcgcg
     8281 ctcttacctt tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc
     8341 gggttcggca gcagatcttt gttataaatc agcgataacg cttcgacagc aatcgggtaa
     8401 gcaatcagct tgccgttgta acgaacggca tcccaggtaa acggatacag cttgtcctgg
     8461 aacgctttgt ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg
     8521 tcgtgcgccc agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc
     8581 tcttccagtt tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc
     8641 ttaccgactt cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta
     8701 ccttcttcga ttttggcgag agtcgaggcg gaaaacatca tcgtcgttaa tgcggataat
     8761 gcgaggatgc gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct
     8821 cgtgaaaaca cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt
     8881 gcccctacgc cccaccgttg ctttgtgtga tctctgttac agaattggcg gtaatgtgga
     8941 gatgcgcaca taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac
     9001 ctcggtttag ttcacagaag ccgcgttctc atcctcccgc ctcctccccc ataaaaaagc
     9061 cagggggtgg aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt
     9121 gctgtcgatg acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat
     9181 gtaacgaaag cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa
     9241 ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg
     9301 attgccgggc ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat
     9361 gacactccgc cagcagaacg cggcgttggt atggtgtttc agtcttacgc gctctatccc
     9421 cacctgtcag tagcagaaaa catgtcattt ggcctgaaac tggcaggcgc aaaaaaagag
     9481 gtgattaacc aacgcgtcaa ccaggtagcg gaagtgctgc aactggcgca tttgctggat
     9541 cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg
     9601 gtggccgagc caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg
     9661 cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt
     9721 tacgtcaccc acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac
     9781 gctggtcgcg tggcgcaggt tgggaaaccg ctggagctgt atcactatcc ggcagaccgt
     9841 tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc tgccagtaaa agtgaccgcc
     9901 accgcaatcg atcaagtgca ggtggagctg ccgatgccga atcgtcagca agtctggcta
     9961 ccggtcgaaa gccgtgatgt ccaggtggga gccaatatgt cactgggtat tcgcccggag
    10021 catctactgc cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag
    10081 caactcggca acgaaacgca aatccatatc cagatccctt ccattcgtca aaacctggtg
    10141 taccgccaga acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg
    10201 ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag
    10261 ccgggcgttt aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga
    10321 aaagcaatga ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg
    10381 ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac
    10441 gttccggtat tggctggaca ggtagcggcg gcgaacaaca gtgtttccag actaccggtg
    10501 ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc
    10561 aggaagtgtg gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg
    10621 tcgcgcaaca gaatgactgg gaagctaccg atccggcctt ccgtgaagca aacgtgcagg
    10681 gtaaaaacct gatcgaatgg ctgccaggct ccaccatctg ggcaggtaag cgcttctacc
    10741 aacgtcatga cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg
    10801 gtctggaaaa catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg
    10861 aagcgggtgg ttcttcctct ttcgccagca acaatattta tgactatacc aacgaaaccg
    10921 cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat caacccgggc ggcacattag
    10981 aactgggtgt cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg
    11041 catcgaaaga cggctggtta ttcactgctg aacatactca gagtgtcctg aagggtttta
    11101 acaagtttgt tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc
    11161 agggttctgg cgtcgcgttt gataacgaaa aatttgccta caatatcaat aacaacggtc
    11221 acatgctgcg tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt
    11281 acgtgggtat gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg
    11341 tcggtattcg cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct
    11401 acgacaacgt cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg
    11461 cacaacaatg gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa
    11521 cctacgccaa gtgggatgag aaatggggtt atgactacac cggtagctct tcaaccaacc
    11581 catactacgg caaggctgtg tcggctgatt tcaacggcgg tagcttcggt cgtggcgaca
    11641 gcgacgagtg gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc
    11701 tgataaggtg cttgcgccgc atccggcaac caatgcctga tgcgacgctg ttgcgtctta
    11761 tcaggcctac aaccgctgcc gaaagtaggc cggataaggc gtttacgccg catccggcaa
    11821 ccaatgccgg atgcgacgct gttgcgtctt atcaggccta caactgctgc cgaaagtagg
    11881 ccggataagg cgtttacgcc gcatccggca accaatgcct gatgcgacgc tgttgcgtct
    11941 tatcaggcct acaactgctg ccgaaagtag gccggataag gcgtttacgc cgcatccggc
    12001 ataaaaacag ctttaagggg cgaaagcccc tctgattatc gggtttagcg cgctattgcc
    12061 tggctaccgc tgagctccag attttgaggt gaaaacaatg aaaatgaata aaagtctcat
    12121 cgctctctgt ttatcagcag ggttgctggc aagcgcacct ggaatcagcc ttgccgatgt
    12181 taactacgta ccgcaaaaca ccagcgacgc gccagccatt ccatctgccg cgctgcaaca
    12241 actcacctgg acaccggtcg atcaatctaa aacccagact actcaactgt cgaccggcgg
    12301 ccaacaactg aatgtccctg gcatcagtgg tccggttgct gcgtacagcg tcccggcaaa
    12361 cattggtgaa ctgacactga cgctgaccag cgaagtgaac aaacaaacca gcgtatttgc
    12421 gccgaatgtg ctgattcttg atcagaacat gaccccatca gccttcttcc ccagcagtta
    12481 tttcacctac caggaacccg gcgtgatgag tgcagatcgg ctggaaggcg ttatgcgcct
    12541 gacaccggcg ttggggcagc aaaaacttta tgttctggtc tttaccacgg aaaaagatct
    12601 ccagcagacg actcaactgc tcgacccggc taaagcctat gccaaaggcg tcggtaactc
    12661 gatcccggat attcccgatc cggttgctcg tcataccacc gatggcttac tgaaactgaa
    12721 agtgaaaacg aactccagct ccagcgtgtt ggtaggacca ctatttggtt cttccgctcc
    12781 agctccggtt acggtaggca acacggcggc accagctgtg gctgcacccg ctccggcacc
    12841 ggtgaagaaa agcgagccga tgctcaacga cacggaaagt tattttaata ccgcgatcaa
    12901 aaacgctgtc gcgaaaggtg atgttgataa ggcgttaaaa ctgcttgatg aagctgaacg
    12961 cctggggtcg acatctgccc gttccacctt tatcagcagt gtaaaaggca aggggtaatt
    13021 acgccccaca gtgctgattt tgcaacaact ggtgcgtctc ctggcgcacc tttttttctc
    13081 ctcctgtact attgttgcgt tattgttgcg cattagttca cttaatttgc tttacatctc
    13141 ccgtaaatat ttttctgcga tacaatgcct ttacgttatg taacggagag ttcggcatgt
    13201 cacaccccgc gttaacgcaa ctgcgtgcgc tgcgctattt tacagagatc cctgccctgg
    13261 agccgcaact gctcgactgg ctgttgctgg aagattccat gacaaaacgt tttgaacagc
    13321 agggaaaaac ggtgagcgtg acgatgatcc gcgaagggtt tgtcgagcag aatgaaatcc
    13381 ccgaagaact gccactgctg ccgaaagagt ctcgttactg gttacgtgaa attttgttat
    13441 gtgccgatgg tgaaccgtgg cttgccggtc gtaccgtcgt tcctgtgtca acgttgagcg
    13501 ggccggagct ggcgttacaa aaattaggta aaacgccgtt aggacgctat ctgttcacat
    13561 catcgacatt aacccgggac tttattgaga taggccgtga tgccggactg tgggggcgac
    13621 gttcccgcct gcgattaagc ggtaaaccgc tgttgctaac agaactgttt ttaccggcgt
    13681 caccgttgta ctaagaggaa aaaaatatgg agtggagtct gacgcagaat aagctgctgg
    13741 cgtttcatcg cttaatgcgt acggataagc caattggcgc gttactgctg ctctggccaa
    13801 cattatgggc gttgtgggtg gcgacaccgg gcgttcccca gctctggatc ctggcggtgt
    13861 ttgtcgcggg cgtctggctg atgcgcgctg ctggttgcgt ggtgaatgat tatgctgacc
    13921 gcaagtttga tggtcatgtt aagcgcacgg cgaaccgacc acttcccagc ggcgcggtaa
    13981 cggagaaaga ggcgcgcgcg ctgtttgtag tgctggtact gatttcattt ttactggtgc
    14041 tgacgctgaa tacgatgact attctgttgt cgattgccgc gctggcgctg gcgtgggtgt
    14101 acccgtttat gaagcggtat acccatctac cacaagtggt gctgggcgcg gcgtttggct
    14161 ggtcgattcc aatggctttt gccgctgtga gtgagtcggt gccattgagt tgctggttaa
    14221 tgttcctcgc caatattctc tgggcggtgg cttacgacac gcagtatgcg atggttgacc
    14281 gcgatgatga tgtgaagatt ggcattaaat ccaccgcaat cctgttcggc caatacgata
    14341 aattgattat tggtattttg cagattggcg ttctagcgct gatggcgatc atcggtgagt
    14401 taaatggctt aggctgggga tattactggt caattctggt agctggcgcg ctgtttgttt
    14461 atcaacaaaa actgattgcc aaccgcgagc gtgaagcctg ctttaaagca tttatgaata
    14521 ataactatgt tggtctggta ctgtttttag ggctggcaat gagttactgg catttctgat
    14581 gatgtaaaaa agccggatga tcatccggct ttcttctggg ttgcctgatg cgctgcgctt
    14641 ctcaggccta cacaacatat cgcaatttat tgaatttgca gatcatggta ggccgaataa
    14701 ggcgtttgcg ccgcatccgg caattctcac tgattaccct tcgccctgtg tcgcactctc
    14761 aatcgtcaaa cgcacgtctg atgtaatcaa ctccgccagc aactgatata ccttcatcgt
    14821 ttctgccggt tcggcgtcgc cgctatcgct gatataccct tcatcacgca gtgtcagcac
    14881 cagagaactg aacaccgcct tgtcgaagaa ctccggcgcg ttgataccgt gcagcacgga
    14941 gagacgttgc gcgacggtgc ggctctcttt ctccagtgta ccgcggttga tcgacgggtt
    15001 ggcactcaac agccagaagg tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc
    15061 cagcagctgt agtgtgcgag aatgcgccgg gttgatatgc aactcatcat cttgcagggt
    15121 aatcagcccc tgacgttgca tctcatttgc cagcgcatca ataacgtccg gtaactcgtc
    15181 gcgatcccag cgcaggaaca gctccgcttt cagcattggg taaagcacat tgacgtgctc
    15241 catcaatacg tcgcgggaga tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg
    15301 cagcaccaac atatgtgcaa tgttgttgcg atagtaggtc atcagcaccg cttgctcgcg
    15361 cggcagaatg atgatgtcgc cgatagtgtc tttctcaact tcaaacttgt tcatttgcag
    15421 cgcgtgatcg ataagctcgc tggcgctggc tgaaggaacg gtagagtccg tggagtaagg
    15481 cacgttgcgc atcagatcca ggtagcagtt gagttgctcg gttaactgct cgcgggtgag
    15541 tgagcgctga cgtgacgcca gaagcgcagt acagcacagg ttcatggcgt ttgccgcgcc
    15601 tgcgttgtta atgcgtacca tcagatcggc agcaatatta ttgaccgtcg gcgttaacca
    15661 ggccggacgc accgcttcga tgggatcgat agattcacgc cagtctggta cgtgctggtt
    15721 aaggtaggtc atcaacggca ttggttcacc gaagttgacg taaccctgac cgagattacg
    15781 cagcttgctt aaaccacgca gcatctgcgg caggctctct ttctctttcg tcgcaccgcg
    15841 cagttctttg gcgtaagtac ccacttccat gacgtgctca taaccgatat agatcggaat
    15901 cagcgtaatc ggacgagtgc cgccacgcag catcgcctga atggtcatcg acagcgtacc
    15961 ggttttcgga tccagcaaac gccccgtacg ggaacgaccg ccttccacga aatactcgac
    16021 ggaataacca cggctgaaca gttcgccgag atactcacgg aaaacggtgg aataaagttt
    16081 attgccttta aacgtacggc gaataaagaa cgcccccaga cggcggaaaa tcggcccggc
    16141 cggccagaag tttaggttga tcccggcggc gatatgcggc ggcaccagcc cttggtgata
    16201 cagcacgtaa gaaagcagca ggtagtccat gtgactgcgg tggcaaggca catacactag
    16261 ctcatggccg tcgtgggcca gctggcgaac gcgctcggcg ttatggacgt tgataccctg
    16321 gtaaagtcgg ttccaggtga agcccagaat gcggtcagtc aggcggatca tctcgtaaga
    16381 gaaattcgcc gcaatctctt ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga
    16441 gatttttttg ctgcgcgctt catcttctac cgctttcgca atggcgcggg aggcgagcag
    16501 cttattaaac agatcctgac gagcaggcag acgtggaccc acggcagcca ggcgttgacg
    16561 ggcaaagtgc atacgcgcca cgcgcgccag tttctgagcg atagttttat ccgtgccgtg
    16621 ttcatccgcc atacggcgca gcgaaactga cggcgagaaa cgcacaaaac tgtcgcgacc
    16681 gagccacagt acagcgaaaa atttctgtac accgttaagc atacgcagcg gcgggttcac
    16741 ttcgcctttt tcacgccccg gcgcgcgacc aaacatcacc gatactggca ccatctgcac
    16801 atccagattt gggttgctgc ggtgcaaatc gagatagtcg tggaacagct taatagactc
    16861 ttctttcggc gtgtaatagg tgaacacacg cggcccgccg tgaatgaaca catagcgcgg
    16921 cagtagcgtg ccatcgattt ccagcggctc taacgggtca ggcaggtcat gtgccagaca
    16981 ctgggcgcgc aacgtcagca agtcagcttt cgagttgtac ggtaaaacgt acataattgg
    17041 acgagaggta tccagcccca gttccggggc aggatctgcc ggaatagact tgctttttac
    17101 caggatgctt aatggtaaat tcagtaattt gtagtaaatt cgtggccagc cggacataaa
    17161 cgatgtaaag cctctggtta ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg
    17221 aaattctgtg gtatccgctc atgtttcgcg cggcgttacg caaacccgaa tcatcggatt
    17281 taacggtaca ctgatattga cgctcataat gtaaaaaggt tctttcaatg gccaataata
    17341 ccactggatt cacccgaatt atcaaagctg ctggctattc ctggaaaggt ttacgcgctg
    17401 catggatcaa cgaagcggca ttccgtcagg aaggcgtagc ggtattgtta gcggtggtta
    17461 tcgcctgctg gctggatgtg gacgcgatta cccgcgtgct gcttatcagc tccgtgatgc
    17521 tggtgatgat tgtggaaatt ctcaatagcg ccatcgaagc tgtggttgac cgaattggct
    17581 ctgaatacca tgagctttcc ggacgcgcta aagatatggg atccgccgca gtgctgattg
    17641 ccattatcgt cgccgtgatt acctggtgca tcctgttatg gtcgcatttt ggataaccct
    17701 tccagatttc gataaatctc tggtttattg tgcagtttat ggttccaaaa tcgccttttg
    17761 ctgtatatac tcacagcata actgtatata cacccagggg gcggaatgaa agcgttaacg
    17821 gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg
    17881 ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa
    17941 catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg
    18001 attcgtttat tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt
    18061 gaaccgcttc tggcgcaaca gcatatcgaa ggtcattatc aggtcgatcc ttccttgttc
    18121 aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt
    18181 atggatggcg atttgctggc agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt
    18241 gtcgcacgta ttgatgacga agttaccgtt aaacgcctga aaaaacaggg caataaagtc
    18301 gaactgttac cagaaaacag cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc
    18361 ttcaccattg aagggctggc ggttggggtt attcgcaacg gcgactggct gtaacatatc
    18421 tctgagaccg cgatgccgcc tggcgtcgcg gtttgttttt catctctctt catcaggctt
    18481 gtctgcatgg cattcctcac ttcatctgat aaagcactct ggcatctcgc cttacccatg
    18541 attttctcca atatcaccgt tccgttgctg ggactggtcg atacggcggt aattggtcat
    18601 cttgatagtc cggtttattt gggcggcgtg gcggttggtg caacggcgac cagctttctc
    18661 tttatgttgt tgctgttttt acgcatgagc accaccgggc tgactgcgca ggcttatggt
    18721 gccaaaaatc ctcaggcatt agcccgtgcg ctggtgcaac cgttgctgtt ggcgttgggg
    18781 gccggggcgt taattgcgct gctgcgtacg ccgattatcg atctggcgct gcatattgtt
    18841 ggtggtagtg aagcagtcct ggaacaggcg cgacgctttc ttgaaatccg ctggttaagc
    18901 gcaccggcgt cgctggcgaa tctggtatta cttggctggt tactcggcgt gcaatatgcc
    18961 cgtgcgccag taattttgtt agtggtcggc aatatcctca acattgtgct ggatgtctgg
    19021 ctggtgatgg ggctgcatat gaacgtgcag ggcgcggctc tggcgacggt tattgcggaa
    19081 tatgcaacat tgctgattgg tctgctaatg gtgcgtaaaa ttctcaaact acgcggaatc
    19141 tccggcgaaa tgctgaaaac agcctggcga ggaaacttcc gtcacttgct ggcgcttaac
    19201 cgcgatatca tgctgcgctc gctgttgctg caactctgtt tcggcgcgat cactgtactt
    19261 ggcgcgcgac tggggagtga cattatcgct gttaacgcag ttctgatgac gctactcacc
    19321 tttaccgcct atgcgctgga tggttttgcc tacgcggttg aagcacattc cggtcaggca
    19381 tacggtgcgc gcgacggtag tcaattgctg gatgtctggc gggcagcgtg ccgccagtcg
    19441 gggatcgtag cgttactgtt ttcggtggtt tatttgctgg caggggaaca catcattgcg
    19501 ttgctgacgt cgttaaccca gattcagcag ctggctgacc gctatcttat ctggcaggtg
    19561 attttgccgt tggtcggcgt ctggtgttat ctgctggacg gcatgtttat aggcgcaacg
    19621 cgtgccgccg aaatgcgtaa cagtatggcg gtggccgccg caggttttgc actgacgctc
    19681 cttacgctgc cgtggctggg gaatcatggt ttgtggctgg cgttaaccgt ctttctggcg
    19741 ttacgcgggc tttctctggc ggctatctgg cggcgtcact ggcgtaacga tacctggttt
    19801 gtcgcaacgt gacggttaaa aattctgaat aaataatcct aagccaaatt gctgactaca
    19861 cttaatctca cgttcagaag aaaagtgaac gtactctcat tcacaaccta acgatgaggt
    19921 cttgattatg aataaagatg aagccggcgg taactggaaa cagtttaaag gtaaagtgaa
    19981 agagcaatgg ggcaaactga ccgatgatga tatgacgatc attgaaggaa aacgtgatca
    20041 gttagtgggt aaaatccagg aacgctacgg ttatcagaaa gatcaggcag aaaaagaggt
    20101 tgatagttgg gaaaaacgcc atgattaccg ctggtaatta atccctcctg cccgacatgt
    20161 acaaggatgt acaccctttt agcgaggttt ctttttcacc tgaatagagt ggtcatgatg
    20221 gcactgttcg ggatgacgac acgcttctac ttctacacat gctgaacata atccatgtgc
    20281 ttcaataaca ttatgccgca gggcaaaacc catttttgcc gccagcgtat gcatgatgtc
    20341 ttccacgcct tcagcacatt cttctttcac cgcgccgcag cgatcgcaaa taaacatggc
    20401 tgaagtatgg gtgggctgat cgaacagatg acagagcaca taactgttgg tggattccac
    20461 cttatgcaca aaaccttgtt caagcagaaa ctccagcgcg cgataaaccg ttggcggctt
    20521 ggcttgcggt tcagcttcgc gcagcaaatc aagcagatca taagcgctga tagcgccatc
    20581 ctgcagactc atcaggcgca acacttcgag acgctgtggg gtcaggcgca cattacgctg
    20641 tgcgcagatt ttttcagcct gcgctaataa ctcctgcgtt gtggtctttt ccattgggca
    20701 cctcgacagt ggggttaaga atgcccttac tttaccatag ttctcgctta aacgccgaga
    20761 acagggtgtc atggctgtca cattgcctga tcgcatattc gttatgctat agtagcgccc
    20821 ccaatttacc agatgcttaa aaagtcgcca tgccagcaga atcacacact gtttatcccg
    20881 cttaccgttt ctcgattgcc ccgatgctcg actggacgga cagacattgc cgttatttct
    20941 tgcgtctgct ttcccgcaat acgttgctgt ataccgaaat ggtgaccaca ggggcgatta
    21001 ttcacggtaa aggtgattac ctggcgtaca gtgaagaaga acatccggta gcgttgcaac
    21061 tcgggggtag cgatccggcg gcgctggcac agtgtgcgaa gctggcagaa gcgcgtggat
    21121 acgatgagat caacctgaat gttggctgcc cgtctgaccg ggtgcagaac ggcatgtttg
    21181 gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg atgcgcgatg
    21241 tggtgtcgat tccggtgacg gtgaaaacgc gtattggcat cgatgatcag gacagctatg
    21301 aatttctctg cgatttcatc aacaccgttt ccggcaaagg tgagtgtgag atgttcatca
    21361 tccacgcacg taaagcctgg ctttctgggt taagtccgaa agaaaaccgt gagatcccgc
    21421 ctctcgatta tccgcgtgtg tatcaactga agcgtgactt tccgcatctg acgatgtcaa
    21481 ttaacggtgg tatcaagtcg ctggaagaag ctaaagcgca tttgcaacat atggatggcg
    21541 tgatggtcgg gcgcgaggcg tatcagaatc cggggattct ggcggcggta gaccgagaga
    21601 tttttggttc ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc atgtatccgt
    21661 acattgagcg tgaactcagc cagggtacgt atctcggcca tattacccgg catatgctgg
    21721 gcttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt gaaaatgccc
    21781 ataaagcggg tgcagacatt aatgtgctgg aacacgcgct caaactggtg gcggataagc
    21841 gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt cgcggggtgt
    21901 tttgctgtta aatcaacaga ttatttttgg catgattctt gtaatgccag caagagattt
    21961 catatttggg agagcatcat gctggaacta ctttttgtaa ttggcttttt tgtcatgctg
    22021 atggtcaccg gtgtttcgtt gctgggtatt atcgccgcgc tgattgtggc gacggccttt
    22081 atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggct gctgttggcg
    22141 gttgcggtgg tgtgggtgat cagggcgatt aaagcaccaa aagtgccgaa atatcagcgt
    22201 tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg ttttatcctt
    22261 agaacaaaat aggaattgat aatcaaatct gtcactattg cgcgtcaaac aaattcatcg
    22321 agctgtaccc tacatacagc cgaattataa aaagaaaggg cttcccaggt ggaagcccta
    22381 tttcttttat ggaatcaata ggctggaacc ttgcgtcgcc cggctttcca gaatctcatg
    22441 cgcacgctgt gcatccttaa gcggatattt ctgctgctcg gcgacatcga ccttaatcac
    22501 accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct cccgcgtggt
    22561 gatatagcct tgcagggaag ggcgtgttac atacaacgag cctttctgat tgagaatgcc
    22621 taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctcaccatta agccgcggcg
    22681 ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc actgaatcgt acaccacacg
    22741 cactttctta ccaccggtga tctctttcaa tcgctcgacc agattctctt cacgatagtt
    22801 aataacctgc cacgcgcccg cttttagcgc actctgcgct ttttgcgcgg ttcctacggt
    22861 gccgataagt ttcgcgccca gggcttttgc ccactggcag gcaatcaagc caacgccgcc
    22921 agccgctgcg tggaacagga actgctcatc gggtttaatt tcataggttt tgcgcagcag
    22981 ataataaacc gttaagcctt tcaggaagga tgccgcagct tgctcaaaag aaattgccgc
    23041 aggcagaatc gccgctttat ctgcattaat gttatgcaca gagctgtaag cgcctaatgc
    23101 cgattgtgca tagactacgc gatcgcctgc cttaatatgc tttacaccac tgccgacttt
    23161 actcacgatg cctgccgctt cggtgcctaa tccgctgggt agcgatggtg gcgggtaaag
    23221 gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt cgacctggat
    23281 ttcattctcc gccggatcgg caggagtgaa ctctacggct tgaagtactt ccgggccacc
    23341 gtgcttgtga aattcaattc gtattgccat gtgtcctcca gaacgtatcg tcagggtctg
    23401 cttcatatgg taaagtttcg acccattctt tatctcggta actccattca ctatggcagg
    23461 aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac aagttgccgg
    23521 gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg gtttaatgct
    23581 ggataacgaa cgctgggacg atgtagccga gcgtgtggtg gcagacgact tttacacccg
    23641 cccacaccgt catatcttta ctgaaatggc gcgtttgcag gaaagcggta gtcctatcga
    23701 tctgattacc cttgcggaat cgctggaacg ccaggggcaa cttgatagcg tcggtggttt
    23761 cgcttatctg gcggagctgt caaaaaatac gccaagtgcg gcgaacatca gtgcttatgc
    23821 tgacatcgtg cgtgaacgtg ccgttgttcg cgagatgatt tcggttgcga atgagattgc
    23881 cgaagccggt tttgatccgc aggggcgtac cagcgaagat ctgctggacc tggctgaatc
    23941 ccgcgtcttt aaaattgccg aaagtcgtgc aaacaaagac gaagggccga agaacatcgc
    24001 cgatgtgctc gacgccacgg tggcgcgtat tgagcagttg tttcagcagc cacacgatgg
    24061 cgttaccggg gtaaacaccg gttatgacga tctcaacaaa aaaaccgctg gcttgcagcc
    24121 gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat ttgcgatgaa
    24181 cctcgtcgaa aacgcggcga tgttgcagga taaaccagta cttatcttct cgctggagat
    24241 gccatcagaa cagattatga tgcgttcttt ggcgtcgctg tcgcgcgttg accagactaa
    24301 aatccgtacc gggcagctcg atgatgaaga ctgggcacgt atttccggca ccatgggtat
    24361 tttgctcgaa aaacgtaata tctatatcga tgattcctcc ggcctgacgc caacggaagt
    24421 ccgttcccgc gcgcgccgta ttgcccgtga acacggcggc atcgggctta tcatgatcga
    24481 ctacctgcaa ctgatgcgcg taccggcgct ttccgataac cgtacgctgg aaattgcaga
    24541 aatctcccgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg tggcgctgtc
    24601 ccagttgaac cgttctctgg aacaacgtgc cgacaaacgc ccggtcaact ccgacctgcg
    24661 tgaatctggc tctatcgagc aggatgcgga cttgatcatg tttatctatc gtgatgaggt
    24721 gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta aacaacgtaa
    24781 cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct tcgacaacta
    24841 tgcggggccg cagtacgacg acgaataata attattttat gaattaggta attaaagcaa
    24901 acacttatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc cgcgctctgc
    24961 gacacaacct gcaacgtctt cgtgaactgg cgcctgccag taaaatggtt gcggtggtga
    25021 aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc gatgctgacg
    25081 cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga atcaccaaac
    25141 ctgtactttt actcgaaggc ttttttgatg ccagagatct accgacgatt tctgcgcaac
    25201 attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag gctagcctgg
    25261 acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg ggcgtaaggc
    25321 cggaacaggc tgaggcgttt tatcatcgtc tgacccagtg caaaaacgtt cgtcagccgg
    25381 tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgcggcgca accgagaagc
    25441 aactcgctat ctttaatacc ttttgcgaag gcaaacctgg tcaacgttcc attgccgcgt
    25501 cgggtggcat tttactctgg ccacagtcgc attttgactg ggtgcgcccg ggcatcattc
    25561 tttacggcgt atctccgctg gaagatcgct ccaccggtgc cgattttggc tgtcagccag
    25621 tgatgtcgct aacctccagc ctgattgccg tgcgtgagca taaagccgga gagcctgtcg
    25681 gttatggtgg aacctgggta agcgaacgtg atactcgtct tggcgtagtc gcgatgggct
    25741 atggcgatgg ttatccgcgc gccgcgccgt ccggtacgcc agtgctggtg aacggtcgcg
    25801 aagtgccgat tgtcgggcgc gtggcgatgg atatgatctg cgtagactta ggtccacagg
    25861 cgcaggataa agccggggac ccggtcattt tatggggcga aggtttgccc gtagaacgta
    25921 tcgctgaaat gacgaaagta agtgcttacg aacttatcac gcgcctgacc tcaagagtcg
    25981 cgatgaaata cgtggattaa tcgttcttta atatttgatt gtctgtgccg gatgcggcgt
    26041 gaatgcctta tccggccaat aaaatcgtac caattcaata agttgatatt ctttcatgct
    26101 cttataaagg tcgtgcatct ggcggatgta cgtttgtcat gagtctcact ctgttgctaa
    26161 ttgccattcg cccctgaaca tcctctcgat cttcgccttc ttccggttta ttgtgtttta
    26221 accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac gcctacgctg
    26281 gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc gacaaagtga
    26341 atttaagtat cggtctgtac tacaacgaag acggaattat tccacaactg aaagccgtag
    26401 cggatgcgga agcgcgcctg aatgcgcagc ctcatggtgc ttcgctttat ttaccgatgg
    26461 aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcg gaccatccgg
    26521 tactgcaaca acagcgcgta gcaaccattc aaacccttgg cggctcaggg gcattgaaag
    26581 tgggcgcaga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc agcgatccta
    26641 cctgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtaagt acttacccct
    26701 ggtatgacga agcgactaac ggcgtgcgct ttaatgacct gttggcgacg ctgaaaacat
    26761 tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg ggtgccgatc
    26821 tcactaatga ccagtgggat tccgtgattg aaattctcaa agcccgcgag cttattccat
    26881 tcctcgatat tgcctatcaa ggatttggtg ccggtatgga agaggatgcc tacgccattc
    26941 gcgccattgc cagcgctgga ttacccgctc tggtgagcaa ttcgttctcg aaaattttct
    27001 ccctttacgg cgagcgtgtc ggcggacttt ctgttctgtg tgaagatgcc gaagccgcag
    27061 gccgcgtact ggggcaattg aaagcaaccg ttcgccgcaa ctactccagc ccgccgaatt
    27121 ttggtgcgca ggtggtggct gcggtgctga atgacgaggc attgaaagcc agctggctgg
    27181 cggaagtaga agagatgcga actcgcattc tggcaatgcg tcaggaactg gtgaaggtat
    27241 tgagcacaga gatgccagag cgcaatttcg attatctgct taatcagcgc ggcatgttca
    27301 gttataccgg tttaagttcc gctcaggttg accgactacg tgaagagttt ggggtctatc
    27361 tcatcgccag cggtcgcatg tgtgtcgccg ggcttaacgc gcaaaatgtg catcgcgtgg
    27421 caaaggcgtt tgctgcggtg atgtgatttt aatcaacagg cggatctctc cgcctgttcc
    27481 tgactccagc catcccctac tattctgtcc cttttttcgc ccacttttac atcatctatt
    27541 ttctataagt ttttgaaaat tagtgttttt aactgttggt acggattgtg cagtaatgcg
    27601 gctgtctatg accatcagga gaaaaacatg gagaacatga cctcgcccgg cacgctgctt
    27661 agtggagata acgcgacctg gcttgaagat tactaccaga cctggttgcg tacgccggaa
    27721 caactcccgg aagactggcg gcgttttttt ctttcacctg aactgactgt ccaaagcgta
    27781 tccggtgata acaacgtcag cggtgcgacc ttaaaaaaac aggctgcgat aatacaactg
    27841 atcaatgcct ggcgcacgca ggggcatctg cgcgcaaagc ttgatccact tggtttgaat
    27901 cccccagctg acgttccctc cctcctgcct ggtttttggg gattaagcga agaagacttg
    27961 ttgcaggagt ttagcgtcac ctttggcgca cacaccacgc aaatgccact taagcaactg
    28021 cttaacctgc tagagcaagc ctgggcgggt agccaggctt atgaacttgc tcatctggaa
    28081 aatcgtgaag aaatcaactg gttgttatca cggattgaga gcagcaatgc gccacaagca
    28141 gatgtacaaa cctgtattgc tcgttttgaa aaactgatgg cggcagagac gttagagcgt
    28201 tatctgcata cccgttatgt tgggcagaaa cggttttctc tggaaggggg cgaaagtgca
    28261 atcccggcgc tcgatacgct gacaaaatgc ctgcgtgcgc aaggtgtgga ggaaatggtc
    28321 attggcatgg cgcaccgggg aagacttaat gttctggtta atctgttgaa taaagacccg
    28381 gcacagcttt ttgctgagtt tgaaggcaag cagacgatcg gcagtggttc aggcgatgtg
    28441 aaataccaca tggggtattc cagcaatctg gaaacacctg ccggttcgct gcatgtggcg
    28501 ctggcgtata acccctcaca tctggagatc gtgaatccgg tggtgctggg ccaggtgcga
    28561 gccaggcagg aacgccgtgg tgaggatggt caggcaaaag tggtgggcgt acttattcat
    28621 ggggattccg cgttgggcgg cctcggcgtc aaccaaacca cgtttaactt atcgcagact
    28681 cagggctacg gcactggcgg tacgctacat ctggtgatta acaatcagat tggcttcacc
    28741 acttcccgct tgcaggatat gcgttcttca cggtactgca ccgatattgc caaaatggta
    28801 gcggccccaa ttatccacgt taatggcgat gacgttgacg ctgtttgcca ggtaatggaa
    28861 ctggcgtgcg aatggcgaga caccttccgg cgcgacatca tcatcgatat ttgctgcttc
    28921 cgtaagcacg gacacaacga aagcgatgag ccacgtctta cccagccaca aatgtatcag
    28981 gcagtggatg ctcaccctgg tacgctcgcc cgttatggtg aatctcttgc ccgccgcggc
    29041 ctgttaactc aggcgcaaca agatgagatg acggcacgct atcgcgactg gctggatagc
    29101 tgccagaaac gtgaaccgca gcctttcaaa cctgcaattc actcttttag cgctaactgg
    29161 tatgggttga ccaaccctca ctggagcgcc ccggtatcta cggcattgcc tcggcaaaag
    29221 ctggcagcgt atggtgaaat catttctaca ctgccacccg atgtggtcgc acatcctacc
    29281 attaaacggc aactgacgtt acgccaggat atggccgcag gtactcagcc aatagactgg
    29341 ggaatggctg aaatgctggc ctatgcatcg cttgtggatg cgggtgttgg tgtgcgtctt
    29401 tctggtgaag actcagggcg tggcacgttc agtcatcgcc atgcagtggt acatcatcaa
    29461 actgaagccc gtcgttatct gccgttacag catatccgcg ctggtcaggc atctttcgat
    29521 gtttatgatt cggtgcttaa tgaagaagcg ctcttagcct ttgaatatgg ctattcaacg
    29581 tctgcgcctc agcaactggt tatttgggaa gcacagtttg gtgactttgc caacggcgct
    29641 caggttgcta ttgaccagtt tatctcgtct ggcgaaacca agtgggatcg ctatagcggt
    29701 ttaaccattc ttctgccgca tggctatgac ggacaagggc cggagcactc ttctgcgcgt
    29761 cctgagcgct ggctgcaact gtgcgcagaa aacaacatgc aggtggtgat gcccagtgag
    29821 tcagcacaaa tgttccattt gttgcgcggt caggcattac gcccaatgcg caaaccgtta
    29881 gtgataatga tgagtaagcg tttacttcgg tttaaaggcg ctatgagcga gctttctgaa
    29941 ttcactgatg gcgcttataa accggtagtc accgatccgc aattgcatca gtcccagaaa
    30001 gttaagcgtg ttattttgtg tagcggccag gtctattacg acgtactgga agcccgtaag
    30061 cagcgtgagt gtgaagatga ggtagcgatt gttcgcctgg agcaactcta cccgttcccg
    30121 gtcgcagaac tgaatgatgt tctggcgagt tggccgaact gctgcgaatg gatttggttg
    30181 caggaagagc cagaaaacca gggcgcgtgg cggcagattc gtcatgagct ggcggcactg
    30241 aaaattaaca ctccttactg gcagtatgca ggccgcccgg cagcggcagc tccggcgaca
    30301 gggtatgggc gtgtacataa acaacaaatc gacgaatttc ttgctgctgc gtttgcagat
    30361 attcagccat gaaaggcgca caaaaataat aaggagaaca ttttatgatc gaaattactg
    30421 tccctgtatt accggaatct gtcacggaag gcacgctgac aacctggtgt aaacaagaag
    30481 gcgagcacgt aaaacgcgat gatgtgattg ccgagctgga aaccgataaa gtcattctgg
    30541 aaataccggc cccgcacgat ggcgtgttaa gcaatattat cgtcagcgaa ggtagcacgg
    30601 tcacgtccgc gcaactgttg gcgcatctta agccgcaagc tgtcatagaa gaaaccgtca
    30661 caccagtcac cgaaaccctg gcgatgcctt ccgcacggct ggaggcacag cgtagtggtg
    30721 tcgaacttgc tgacgttgcg gggagcggga gaaatggacg tatcctgaaa gaggatgtac
    30781 agcgcgttac tcctgcacca gtcattcagc ctgaacgggt ggcggagatc gcgccagcta
    30841 aacccttgac accgggtgct cgtcaggaac gtcgtgagcc gatgtcgcgc ttacgtcagc
    30901 ggattgctga gcgtctgctg gcttcccaac aaaataacgc cattcttacc acattcaacg
    30961 aagtaaacat gcagagcgtg atggacttac gcgcccgctg gaaagatcgt tttgccgaga
    31021 agcacggcgt gaagctgggc tttatgtcct tctttgtgaa ggccgttacc cgggcgctgg
    31081 agcgcttccc tgtagtgaat gccagcgttg atggcaacga gattatctgg cgtgattatt
    31141 gcgatatcgg cattgcagtg agcagcaacc gcggcctggt agtgcctgtg ttacgcaatg
    31201 cgcaatcact ctctttggtg gaaattgaac ggcaaattgc cgaatacgcc acacaggcac
    31261 gcaatggcaa attgccgctg gaggcgttac agggcggcac gttctctatc accaacggcg
    31321 gtacttttgg ttcgatgatg tcaacgccaa tcattaaccc gccgcagtcg gccattcttg
    31381 ggatgcatgc catcacgccg cgcccggtcg cagaaaacgg ccaggttgtt attcgtccca
    31441 tgatgtacct tgctctcagc tatgaccatc gcattatcga tggtcaggaa gccgtgcaga
    31501 cattggtggc aatacgtgag ctactggaat caccggaaca actgttgctg gatctttaag
    31561 gagggcgccg atgagtacaa tttttgatgt ggcagttatg gggggtgggc caggtggtta
    31621 cgtagcggca ctgcgggctg cgcagaatgg gctttccgta gtatgtattg atgatggtgt
    31681 taatgcacag ggcgaacctt cgccgggcgg tacgtgtctc aatgtcggct gtattccgtc
    31741 aaaatcgttg ctgcaatctt ccgaactgta cgcgcaagtg cagcatgagg cgagcattca
    31801 tggcgttaat gttgacggcg tatctttcaa tgcagctgcg atgattcaac gtaaagacgc
    31861 catagtaagc cgcctgacaa tgggcatcgg cttgctgttt aagaaaaata aagtgaagta
    31921 cctgtgcggc ctcgcaacgt tagaacatgc tcgggatgag atttggcaat tacgtgtaaa
    31981 cgatcaacaa atccgcgccc gtaatgtggt gattgcaaca ggttctcagc cgcgtcaact
    32041 gcctggcgtt accattgata atcagcaaat tctcgataat cgcggcgccc tagcgttaac
    32101 cgctgtgccg ccgcgtctcg gtgtgatcgg cgctggcgtg attggccttg aactggggtc
    32161 tgtgtggaat cgggtcggtt cagacgtcac gttgctggag atggcgccaa cgttcttgcc
    32221 tgcactggaa gctcgcctgt cgaacgaagt ccgtaaagca atgattgcca gtgggatgaa
    32281 aatgcaactg gcagtagaga ttgaggctat tgaacaacgg gatgacggtg tgcatgtgcg
    32341 ctggcggcag ggtgagaagc gagaagaaag ccgcttcgat aaattaattc ttgccattgg
    32401 acgtgtaccg cgactttctg gtgtcgatct cgtgcaactc ggtcttgaag ccgacaaccg
    32461 tggcggtatt gccgtcgata acttatgccg taccgggaaa gccgggctgt gggcaattgg
    32521 tgatgtcgtg cgagggccga tgctggcgca taaagcgatg gctgaaggtg tggtggttgc
    32581 ggatcaaatt gccggactgg cggtagagcc gattaatttt gcgctgatcc cttcggtgat
    32641 ttacacccaa ccggaagttg cgtgggtagg ggaaaacgag gcctcactga aagccgctgg
    32701 cagagtcttt aacaaaggaa attctctgtt tgccggtaac ggtcgcgcct tagcattagg
    32761 ccaggaaggt gggcgttgca cgttatacag cgataaacac actgaccgcg tacttggcgg
    32821 agcgattgtt ggcccgcagg catcggagct gatcaatgaa attgcgctgg cgatgacttt
    32881 tagcgcctct ggcgaggata ttgcctgtgc gatccatgcg catccgacct taagcgaagt
    32941 catccacgaa gcagcaatgg ccttgaataa taaagcatta cacggataac acataccact
    33001 gagggaatat atgaatttac atgagtatca ggccaaatcg ttactggctg gcatggggat
    33061 gccgtgtcca aaagagatag ccatccaaca gatttcgcaa ctggcagatg cctggcaaca
    33121 tattgcttgc ccgagtaaag gcgcggtact taaagcgcag gtacatgctg gtgggcgcgg
    33181 taaagctggc ggcgtaaaag tgcttaagca gctgccggaa gcacaggctt ttgtacagca
    33241 aatgttggga tcgcaactgg tgacttatca gaccgggcca gaagggcagt atgtcagcag
    33301 tattttgctg tgcgaaaaca tctatccggt acgccaggaa ctctattttg gcatggtggt
    33361 ggatcgtgaa agccagcggg tcacgtttat tgtcagcccg gaaggtggtg tggaaattga
    33421 aaaagtcgcc catgagacac cggagaaaat cagcagtgtc agcattgatc cactgacagg
    33481 cgtgcagcct tgccatatcc gcgaaatgtt tgctgttctg caactggaac acgggctatt
    33541 tgctaccttt agtcgcctgg ttaatcaggc gtggaaagcc tttaacgaac tggatttcgc
    33601 cctgctggaa attaaccctc tggtgttgcg ggaaacgggc gaatttatgt gtgcagacgc
    33661 gaaagtatcg ctggatgata acgcgctgta tcgtcacccg gaactccagg tgctacgtga
    33721 tgaaacccag gaagatccac gcgaaagcca ggcagcgaag cttgatctta attatgtctc
    33781 gctggacgga aatatcggct gcatggtcaa tggtgcaggg ctggctatgg caaccatgga
    33841 catcatcaag ctatacggtg aacaacccgc aaacttcctg gatgttggcg gtggcgcaac
    33901 ccaggagcgc gtcagtgaag cctttcggct gattgtttcc gacagcaaag tgaaagccat
    33961 tctggttaat atttttggtg ggattgtccg ttgcgacatg atcgccagag ccattattca
    34021 tgctctgaat gaggcccgta tcaccctgcc tgtcgtggta cgtctctccg gtaataacgc
    34081 tgccgaaggg cagcggttac tggcagaaag tggcctgacg gtggaagccg tcaattctct
    34141 ggatgatgcc gctaaacgca tcattgcgtt actgaattaa caggaggtta agatgagcgt
    34201 tttgatcaac aaacacacgc gggtactggt gcagggcatt accggcaaaa acggcacatt
    34261 tcatacagag caggctattg cgtatggcac gcagattgtg ggtggagtga cgccaggtaa
    34321 gggcgggcaa cggcatctgg atcgcccggt ttttgattca atgaaagaag cgatgcgaca
    34381 aactgaggct gatgccagcg tgatttatgt gcctgcaccg tttgtgctgg attctgttgt
    34441 tgaggctatt gaagctggcg tcaaattggt tgtggtgatc actgaaggtg taccgacact
    34501 ggatatggtg aaagttcgtc ggctgcttga ctgccacccg gatgtgcggc tgattgggcc
    34561 aaactgccca ggagttatca cgccggggga gtgcaaaatc gggattatgc ctggtgaaat
    34621 ccaccgccca gggcgtattg ggattgtttc ccgctccggt acattaacct acgaagctgt
    34681 agcgcaaacc acggcgctgg gacttgggca atccacctgt attggtattg gcggcgatcc
    34741 ggttccgggg actaacttta ttgatgcgct gcgtttattc gaaaatgatc cgcaaacaga
    34801 cgcggtaatt atgatcggtg aaattggtgg taatgcggaa gaacgggccg cggaatttat
    34861 tcgccatgaa atgaataaac cggtcgtagg ttatattgcc ggtgttactg caccaaaagg
    34921 aaaacgcatg gggcacgtcg gagccattat ttctggcggt agcggcagtg cagaggataa
    34981 attccgtgcg ttcgataaag caggcatggc atggacaaga aacccggcgt tgcttggcga
    35041 aacgttatgg aatgttatta agtaatccat aaatgaatat caagggagca tatcgctccc
    35101 tttttttatc tgtgtggtaa gagaatcacc ttttatttac tgtgcggaaa accgcgcaca
    35161 aaataaaaag aataaataat aaaatcatta aataacaaag cattattaaa aaattaaaga
    35221 tggcatagct cctgcaatag ccaggcaggt tttttatttt tcacaatgag gtgaatatgt
    35281 catcaatatc acatggcgca ccacaaaagc gtcgaattat ccctaatccc ggcctgtggt
    35341 tggcgatcat tgccggaatt attattactc ttctgcctct gggcgatacc ttgcccgtcg
    35401 ctggacaaaa tatgatcgcc attctggtgt ttgccattat tgtctggatc agcgaagcga
    35461 tggattacac cgccagtgca attgttattt ccgcactgat tatctttatg gttggttttg
    35521 ccccggatat gaatcacccg gacacgatcc ttggcaccgc gaaagcgctg aaaatgactc
    35581 tgtcgggttt ttctaattct gctctggcgc tggtggcagc agcaatgttt atcgcggcgg
    35641 caatgaccat taccgggctg gataaacgca ttgcgctgtt tactatgtcg aaaataggtg
    35701 ccagcagccg cagcattatt atcggtgcca ttgtggtgac catcgtcctg agcctggtcg
    35761 tacccagtgc cactgcccgt acggcttgcg tggtgccgat tatgatgggg gtcattgcgg
    35821 catttaaggt ggataaacat tcacgtttag cggcgtcaat gatgattgtt atcgcccagg
    35881 cgaccagtat ctggaacgtt ggtattcaaa cttcggcggc gcaaaacctg ctttctatcg
    35941 gttttatcaa taaaactttc ggcgccgggc attctgtcag ctggcttgac tggctgttag
    36001 cgggcgcgcc ctggagcctc accatgtcgg cgatcctcta tttcctcgca cgtaaattgt
    36061 tgccgccaga aacggaagct gttgaagggg ggagcgaggc cattaaaaaa gcacttgcag
    36121 agttagggcc aaccactggt aaagaaaaac gtttgatcgg tatttcgcta ttgttactgc
    36181 tcttttggtc tactggcgga aaattacaca gtattgatac cacctctgtc acccttgccg
    36241 gactggcgat tatgttgctg ccaggcatcg gtgtaatgag ctggaaagag gtcgaaaaac
    36301 gtgtgcagtg gggcacgttg cttatgtttg gtattggtat cagcctgggg tccacgctgc
    36361 ttgatacgca ggcagcttca tggatggcga actatgtggt gaaaggtttt ggtctcgatg
    36421 gcttgccatc attagccatc ttcgcgattc tcgccgcttt cttgataatt attcacctgg
    36481 ggtttgccag tgccactgcg ctaactgccg cattgctgcc catcctgatt agcctgttaa
    36541 gcagtctgcc gccagaactc ggcgttaacc cggtcggcat gaccattctg ctcgccttca
    36601 gtgtcagttt tggcttcatc ctgccgatta acgcgccaca aaatatggtg tgtatgggta
    36661 cggatacctt cacgccacgc cagtttaccc gtgtgggact gtacctgacg gtaatcggct
    36721 acctgttgtt gctactgttt gccgccacct ggtggaaaat tttaggtcta atgtgaggaa
    36781 accaacgatg tcattacaaa ttactgttga aaaagtgcgt tggttagctg ccgggctgct
    36841 ggaacttaat ggctgtgatg ccgatatcgc tcaggatgtg gcggaacata tgattgaagc
    36901 ggaacgttat ggctttgcca gccacggcgt cacgctgcta ccgaaatatc tggaaaatat
    36961 cgctcgtggc gatgtaaccg cgaatgcccg cccggaatgc ttaacaagtg aaggtaattt
    37021 acaacgtttt catgcccaca atggctttgg tcaacacgcc ggaaaagtgg cggtaaatgc
    37081 tgccatcgaa caagcaaaac accgcggttt ttgcctgtta acgttgtgcc atgcgcatca
    37141 tttagggcat atggggcatt acgggcaaat ggttgctgat caaggcctgg cgatgctggc
    37201 catgagtaac gtcaccgggc gtcctgcact ggtcgccccg tggggcggtg cggaaccgcg
    37261 tatgaccact aaccctttct gctttgcctg gccgttcagt gatgggcgtc cgccgattct
    37321 ggttgatttt gccaccagta gcatggcgct aaataaagca cgggtaatgt cgtccaccgg
    37381 gaagcaggcc gcgccgggac aattgattga tgcacagggg aatccaagca atgatcctgg
    37441 ggtgttattc agcaatcctc caggggcgtt actgccattt ggtgagcata aaggatttgg
    37501 cctggcactg atgattgaaa tgatggcggg gatcttatct ggtggtgaca cgatcgccga
    37561 agatcatcag acggatggat ctgcgcataa tcatcttttt gcgttgatta tcgatcctga
    37621 tcaattcacc gatctggcag gggagaaagg gcagttcttt gcggattatc tgttagctac
    37681 gcagcctcaa cctggcggca atccggtgat ttaccccggt atgccagaag ctgccaaccg
    37741 ggaacgcaat gcgaagatga ttacgatccc ggtatcattc tggtcctggg tagttgaaca
    37801 ttacgcccgc aaaggtatcg atttggggtt acatcctcag gaatcagcat tagctggatg
    37861 attctggtcg ataaacttgc ggttgaatct gatgtttgtt gagtttgtca tataaagtct
    37921 tgcgcggcag ctgtaatagc cgcgctgtca aactgacctg gccttccgtt tgtcgcagca
    37981 tatcttcgat taactttttc tcgaaagcat caatgcaggc ggtaagacca ctctcttctt
    38041 gctgagtcat tggcactggc tggtggtggg tcagtaaacc gagaacaaaa cgctctgcat
    38101 tgtgtttaag ttcacgtaca ttgccaggcc aactttgtga ctgcaaccag gccagtaaca
    38161 tgggagaaat atcaggcaga ggacgattat atttttgtgc cgcctgactg gcgaaccagt
    38221 aaaacagttc agggatatct tctcgtcgct ggcgtagagg tggaatattc aggctaacca
    38281 cattcagacg ataatagaga tcgagacgaa acagatgttc ttcgctgcga cgcagtaaat
    38341 cttccttggt cgctgcaata acacggcaat tcaccggaat taattgattg gcacctaatc
    38401 gttcaatggt tctttcctgc aatacacgta gcagttttac ctgcattcct gatggcattc
    38461 cctctatttc atcgagaaat aacgtaccac catttgcatg ttcaattttt ccaatacgct
    38521 ttttaaccgc accggtaaat gcgcccgcct catgaccaaa tatttcgctt tcaaacaacg
    38581 tttctggtaa gcctgcacag tttaaggcaa caaactgccc ctgacggcgg gtactccagt
    38641 gatgcagcat tctggcgacg acttctttac cacaacctgt ttcaccataa ataaggacat
    38701 cggcaccggt atctgcgaca ttaaggatca ttttacgcag ttgttgcatc tgcggactac
    38761 gaccaatcag caccgggcca ttttcctgtt gcagattcgc cagtaattgc tgattttcta
    38821 aaaccaatcg ccgtttttct accgctctgg caataatact caataattta tctgagctac
    38881 ttggtttctc aatgaaatcg aatgcaccat tacgcatggc atccaccgcc atttccacgt
    38941 caccgtgtcc ggtaattaaa ataaccggaa ttttgctatc catttcgttg atgtaactga
    39001 ggagctctag tcctgatatt ccaggtaacc tgacatcggt cagtataata atgttggcct
    39061 gactttgaat aaggggaata ctgtgttctg catcgcaaca ggaaataaca cgataaccag
    39121 ccagcgtgag cgtttgttca caggcaaaac gtacatcgtc actatcctca atataaacta
    39181 tagtaatttc actggattta ttattcatgt tcttctcccc attcaggcca ggttaatgtc
    39241 attactgcgc cgccttcagg atgattactg gcgtggagtg cgccgttcga atttcggaca
    39301 atttcgctga ctattgccag tcccaacccc attcctctac gtttagtggt aaaaaatggt
    39361 tcaaatatgc gatcgacaac ttcaggtgca aaaccgccac cgctgtcttt aatggtaata
    39421 ataactttgt tatttgcctg gcgaatggcg atatccagtc gtttgtaaga gctaccttcc
    39481 atagaatcta aggcgttgct aattaaatta ctgaatatct gttcaagacc gagctcatcg
    39541 caattgacaa ataacggcat ggatggggct tttattcgtc gctcaatgtt atttttctcc
    39601 atgctgtgat taagtaacgc cacggcgcta tacatcactt tgatgacatc ggcagaacct
    39661 ttaggtacgc gatggcgcga ggcaaatgct ttaagttcgg aaatgagctg cgtcatccgc
    39721 tcaatcactg aaataatatg tttcagattc tgttcaacct gcggatacat ctcttttttt
    39781 agcatagtac gcgcgttatc agtaagtgcg tgaatggcgg ctaacggttg attttgttca
    39841 tgggcaattt cggttgccat ctgtccaagc gccgccagtt tgctggactc tgccagttca
    39901 ctacgcgtaa tttgtaatac ttgttcagct tgactgcgct cttttatctc ctgtattaat
    39961 ttttgattga tcaattccag ggcagatgta cgctctttaa cctgtttttc taatgtttca
    40021 ttcgcaagtg ttaataattg ttgtgcatga gatcgcattc tccagtattt aataaataac
    40081 aaaaacagca ggtaaataat tagtgtaatg actaacaaaa tagcccatga ccagtacagg
    40141 ttatctaagg ggaccata
//
LOCUS       DAZFRG010000013        39763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFRG010000013 DAZFRG010000000
VERSION     DAZFRG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.013"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1705
                     /gene="adeD"
                     /locus_tag="ACHKUC_000500"
     CDS             <1..1705
                     /gene="adeD"
                     /locus_tag="ACHKUC_000500"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO6616817.1"
                     /translation="AVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVGAE
                     YADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHEIV
                     NVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQVT
                     GLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIENCHESYQ
                     LEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEGHI
                     DALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLSPGKQADIVLLSDVRKVTVQ
                     QVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQPVSASDFALQFTPGKRYRVIEV
                     IHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGALAATI
                     SHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTAQS
                     LAEQIDTLKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLEFT
                     E"
     gene            complement(1752..3143)
                     /gene="uhpT"
                     /locus_tag="ACHKUC_000501"
     CDS             complement(1752..3143)
                     /gene="uhpT"
                     /locus_tag="ACHKUC_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO6616818.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3281..4600)
                     /gene="uhpC"
                     /locus_tag="ACHKUC_000502"
     CDS             complement(3281..4600)
                     /gene="uhpC"
                     /locus_tag="ACHKUC_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6616819.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4610..6112)
                     /gene="uhpB"
                     /locus_tag="ACHKUC_000503"
     CDS             complement(4610..6112)
                     /gene="uhpB"
                     /locus_tag="ACHKUC_000503"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO6616820.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6112..6702)
                     /gene="uhpA"
                     /locus_tag="ACHKUC_000504"
     CDS             complement(6112..6702)
                     /gene="uhpA"
                     /locus_tag="ACHKUC_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO6616821.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6775..7065)
                     /gene="ilvN"
                     /locus_tag="ACHKUC_000505"
     CDS             complement(6775..7065)
                     /gene="ilvN"
                     /locus_tag="ACHKUC_000505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6616822.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7069..8757)
                     /gene="ilvB"
                     /locus_tag="ACHKUC_000506"
     CDS             complement(7069..8757)
                     /gene="ilvB"
                     /locus_tag="ACHKUC_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO6616823.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8863..8961)
                     /gene="ivbL"
                     /locus_tag="ACHKUC_000507"
     CDS             complement(8863..8961)
                     /gene="ivbL"
                     /locus_tag="ACHKUC_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO6616824.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            complement(9206..9280)
                     /gene="ysdE"
                     /locus_tag="ACHKUC_000508"
     CDS             complement(9206..9280)
                     /gene="ysdE"
                     /locus_tag="ACHKUC_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO6616825.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9362..10546
                     /gene="emrD"
                     /locus_tag="ACHKUC_000509"
     CDS             9362..10546
                     /gene="emrD"
                     /locus_tag="ACHKUC_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO6616826.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(10554..11051)
                     /gene="yidF"
                     /locus_tag="ACHKUC_000510"
     CDS             complement(10554..11051)
                     /gene="yidF"
                     /locus_tag="ACHKUC_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6616827.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11048..11410)
                     /gene="yidG"
                     /locus_tag="ACHKUC_000511"
     CDS             complement(11048..11410)
                     /gene="yidG"
                     /locus_tag="ACHKUC_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO6616828.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11400..11747)
                     /gene="yidH"
                     /locus_tag="ACHKUC_000512"
     CDS             complement(11400..11747)
                     /gene="yidH"
                     /locus_tag="ACHKUC_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO6616829.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(11807..13249)
                     /gene="yidJ"
                     /locus_tag="ACHKUC_000513"
     CDS             complement(11807..13249)
                     /gene="yidJ"
                     /locus_tag="ACHKUC_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6616830.1"
                     /translation="MVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCTPARAGLFT
                     GIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWD
                     ADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVGFLQQP
                     ARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKAEDDLANKPEHHRLWAQA
                     MPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHGEMMGAHK
                     LISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGEN
                     ILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPN
                     EMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLRPWRKDAQPRWMGAFRPR
                     PQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(13297..15012)
                     /gene="yidK"
                     /locus_tag="ACHKUC_000514"
     CDS             complement(13297..15012)
                     /gene="yidK"
                     /locus_tag="ACHKUC_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO6616831.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAIISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            15179..16045
                     /gene="yidL"
                     /locus_tag="ACHKUC_000515"
     CDS             15179..16045
                     /gene="yidL"
                     /locus_tag="ACHKUC_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6616832.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYAVGKKDPQRRQIEKLIATLHANLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(16135..17796)
                     /gene="yidE"
                     /locus_tag="ACHKUC_000516"
     CDS             complement(16135..17796)
                     /gene="yidE"
                     /locus_tag="ACHKUC_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO6616833.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(17993..18421)
                     /gene="ibpB"
                     /locus_tag="ACHKUC_000517"
     CDS             complement(17993..18421)
                     /gene="ibpB"
                     /locus_tag="ACHKUC_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO6616834.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(18533..18946)
                     /gene="ibpA"
                     /locus_tag="ACHKUC_000518"
     CDS             complement(18533..18946)
                     /gene="ibpA"
                     /locus_tag="ACHKUC_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO6616835.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(18940..19012)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            19252..19584
                     /gene="yidQ"
                     /locus_tag="ACHKUC_000519"
     CDS             19252..19584
                     /gene="yidQ"
                     /locus_tag="ACHKUC_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6616836.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQETNNVI
                     PPAKMPAN"
     gene            complement(19588..20802)
                     /gene="yidR"
                     /locus_tag="ACHKUC_000520"
     CDS             complement(19588..20802)
                     /gene="yidR"
                     /locus_tag="ACHKUC_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO6616837.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHTKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRIYPGLVNIPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            20903..22001
                     /gene="cbrA"
                     /locus_tag="ACHKUC_000521"
                     /pseudo
     CDS             20903..22001
                     /gene="cbrA"
                     /locus_tag="ACHKUC_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
     gene            complement(21962..23299)
                     /gene="dgoT"
                     /locus_tag="ACHKUC_000522"
     CDS             complement(21962..23299)
                     /gene="dgoT"
                     /locus_tag="ACHKUC_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6616838.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(23374..24522)
                     /gene="dgoD"
                     /locus_tag="ACHKUC_000523"
     CDS             complement(23374..24522)
                     /gene="dgoD"
                     /locus_tag="ACHKUC_000523"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO6616839.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(24519..25136)
                     /gene="dgoA"
                     /locus_tag="ACHKUC_000524"
     CDS             complement(24519..25136)
                     /gene="dgoA"
                     /locus_tag="ACHKUC_000524"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6616840.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(25120..25998)
                     /gene="dgoK"
                     /locus_tag="ACHKUC_000525"
     CDS             complement(25120..25998)
                     /gene="dgoK"
                     /locus_tag="ACHKUC_000525"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6616841.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTGWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAADAFAAGLEHGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVDHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(25995..26684)
                     /gene="dgoR"
                     /locus_tag="ACHKUC_000526"
     CDS             complement(25995..26684)
                     /gene="dgoR"
                     /locus_tag="ACHKUC_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO6616842.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            26963..27619
                     /locus_tag="ACHKUC_000527"
     CDS             26963..27619
                     /locus_tag="ACHKUC_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616843.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPHGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(27663..28475)
                     /gene="yidA"
                     /locus_tag="ACHKUC_000528"
     CDS             complement(27663..28475)
                     /gene="yidA"
                     /locus_tag="ACHKUC_000528"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO6616844.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(28590..28988)
                     /gene="yidB"
                     /locus_tag="ACHKUC_000529"
     CDS             complement(28590..28988)
                     /gene="yidB"
                     /locus_tag="ACHKUC_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO6616845.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            29106..29207
                     /locus_tag="ACHKUC_000530"
                     /pseudo
     CDS             29106..29207
                     /locus_tag="ACHKUC_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29228..31642)
                     /gene="gyrB"
                     /locus_tag="ACHKUC_000531"
     CDS             complement(29228..31642)
                     /gene="gyrB"
                     /locus_tag="ACHKUC_000531"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO6616846.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(31671..32744)
                     /gene="recF"
                     /locus_tag="ACHKUC_000532"
     CDS             complement(31671..32744)
                     /gene="recF"
                     /locus_tag="ACHKUC_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO6616847.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(32744..33844)
                     /gene="dnaN"
                     /locus_tag="ACHKUC_000533"
     CDS             complement(32744..33844)
                     /gene="dnaN"
                     /locus_tag="ACHKUC_000533"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO6616848.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(33849..35252)
                     /gene="dnaA"
                     /locus_tag="ACHKUC_000534"
     CDS             complement(33849..35252)
                     /gene="dnaA"
                     /locus_tag="ACHKUC_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO6616849.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            35549..35629
                     /locus_tag="ACHKUC_000535"
     CDS             35549..35629
                     /locus_tag="ACHKUC_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616850.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            35859..35999
                     /gene="rpmH"
                     /locus_tag="ACHKUC_000536"
     CDS             35859..35999
                     /gene="rpmH"
                     /locus_tag="ACHKUC_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO6616851.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            36016..36375
                     /gene="rnpA"
                     /locus_tag="ACHKUC_000537"
     CDS             36016..36375
                     /gene="rnpA"
                     /locus_tag="ACHKUC_000537"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO6616852.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            36339..36596
                     /gene="yidD"
                     /locus_tag="ACHKUC_000538"
     CDS             36339..36596
                     /gene="yidD"
                     /locus_tag="ACHKUC_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO6616853.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            36599..38245
                     /gene="yidC"
                     /locus_tag="ACHKUC_000539"
     CDS             36599..38245
                     /gene="yidC"
                     /locus_tag="ACHKUC_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO6616854.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            38351..39715
                     /gene="mnmE"
                     /locus_tag="ACHKUC_000540"
     CDS             38351..39715
                     /gene="mnmE"
                     /locus_tag="ACHKUC_000540"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO6616855.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNNVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         9610 a        10857 c        10197 g         9099 t
ORIGIN      
        1 agccgtttcc cgtggagacg ctgttgccga ttatattatt gataatgtct ctattctcga
       61 cctgatcaat ggcggagaaa tttccggccc aattgtgatt aaaggacgtt acattgccgg
      121 tgttggcgca gaatacgctg atgctccggc tttgcagcgg attgatgccc gcggcgcaac
      181 ggcggtgcca gggtttattg atgctcacct gcatattgaa tccagcatga tgacgccggt
      241 cacctttgaa accgctaccc tgccgcgcgg cctgacgacc gttatttgcg accctcatga
      301 aatcgtcaac gtgatgggcg aagccggatt cgcctggttt gcccgctgtg ccgaacaggc
      361 aaggcaaaac cagtacttac aggtcagctc ttgcgtaccc gctctggaag gctgcgatgt
      421 taacggtgcc agttttaccc ttgaacagat gctcgcctgg cgggaccatc cgcaggttac
      481 cggccttgca gaaatgatgg actaccctgg cgtaattagc gggcagaatg cgctgctcga
      541 taaactggac gcatttcgcc acctgacgct ggacggtcac tgcccgggtt tgggtggtaa
      601 agaacttaac gcctatattg ctgcgggtat tgaaaactgc cacgaaagtt atcagttgga
      661 agaaggacgc cggaaattgc aactcggcat gtcattgatg atccgtgaag ggtccgcagc
      721 ccgcaatctc aacgcgctgg caccgttgat caacgaattt aacagcccgc aatgcatgct
      781 ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa ggacatatcg atgccttaat
      841 tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg gcatatcgcg tcgccagctg
      901 gtcgacggcg cgccattttg gtctgaatca cctcggctta ctgtcacccg gcaagcaggc
      961 cgatatcgtc ctgttgagcg atgtgcgtaa ggtcacggtg cagcaggtac tggtgaaagg
     1021 cgagccgatc gatgcacaaa ccttacaggc ggaagagtcg gcgagactgg cacaatccgc
     1081 cccgccatat ggcaatacca ttgatcgcca gccagtttcc gccagcgact ttgccctgca
     1141 atttaccccc ggaaaacgct atcgcgttat tgaggtaatc cataacgaac tgatcacgca
     1201 ctcccgctcc agcgtctaca gcgaaaacgg ttttgatcgc gatgatgtgt gctttattgc
     1261 cgttcttgag cgttacggac aacgactggc tccggcctgt ggtttgctcg gcggctttgg
     1321 cctgaatgaa ggtgcgctgg cggcgacgat cagccatgac agccataata ttgtggtgat
     1381 cggtcgtagc gcagaagaga tggcgctggc ggtcaatcag gtgattcagg atggcggcgg
     1441 gctgtgcgtg gtccgtaacg gccaggtaca aagtcatcta ccgttgccca ttgccgggct
     1501 aatgagcacc gacacggcgc agtcactggc ggagcaaatt gacaccttga aagccgccgc
     1561 ccgtgaatgc ggtccgttac cggatgagcc gtttattcag atggcgtttc tttccctgcc
     1621 agtgatcccc gcgctgaaac taaccagtca ggggctgttt gatggcgaga agtttgcctt
     1681 cactacgctg gaattcacgg aataataaaa aaaagcccgg cgtcatgccg ggcaaaagtc
     1741 accagttacg tttatgccac tgtcaactgc tgaatttttt tctcgcggcg gattttgcgt
     1801 tcttccatca ccgccactat cgccatcagg cagatacaac caatagcggc gatatccagc
     1861 gcggcgaagg tgcctgccca gccggtaagg ccaaataccg gcgtcccatc ggcaatcatt
     1921 cccagaccta acttggcaaa gctgtcacca atcaggtaag cgaaggtgcc tttaatacca
     1981 tcggcagcgc caatcgcttt tttaggtaca aagccaacag cagccacacc aatcaacaat
     2041 tgcgggccaa agaccaggaa acccaacgca aagagagaag ccaggtagat atattggttg
     2101 ctggcgtgtt gatacacacc gagcgtggcg ataatcagcg ccagcgcgat acaggccacc
     2161 aggccacggc gaccgttcgc caggtcagag agccagcccc acagcagcgt accgaccagc
     2221 gcaccagctt caaacagcgt aaagccctga atcgccaccg ctttagagag tttcagttcc
     2281 tggaacgcat acacggttga ccactggtcg ataccaatac gcaccacata gaggaaaatg
     2341 ttggcaaagc acagcagcca gatcactttg tttttcagca catactcaac aaagatctgc
     2401 cacttggtca tatcggtaga ttctgtctct ttatcctctt cactgatctc ctcgccaaac
     2461 agctcttcag ctttgccgag gccataagat tccggggagt cgctgccgta acgcagacca
     2521 ataaaaccaa caatcagcgc gataatcgac gggaaaataa acatgccgat gacatgacca
     2581 tcgaacaggt aatttgcgcc gaacagcgcc acacctgctg cgcctgcacc accaaggttg
     2641 tgagaaatgt tccagaaacc gaggaatgtc ccgcgtttac gacgcggcgt ccatttggtg
     2701 atggtggagt aactgcacga accgccggta ctctggaaaa agccgcttaa ggcgtagaag
     2761 gcaatcatca ggaacaggct aaccgagccg ctgcccatac tggcgctgaa gcccagcata
     2821 caaatggcag acaggatcag catgaacggc aggaattgtt tggtgttttt gccgtcggcg
     2881 tagtaagaaa ccagcgtttt acccacgcca taagtgatgg agaagcccag gccgatcatc
     2941 cccagctgcg tcatgctcaa cccgtaggtc gagatcatgt cgttctgcgc gatgttaaag
     3001 ttcttgcgaa tcaggtacat cgtaaggtag ccgataaaga ccaccaggta ggattgcatg
     3061 aacggtttga accacatttt gcgccgcact tcgagcggaa ggtccagggt cggcttgcga
     3121 acctggttta agaaagccag catgggttac tcctgaaatg aatacctgcg tgaggcatgc
     3181 attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc caggcaaaac ctggaaaatt
     3241 tcttagtttt gccggatatg aagtgaaaag gtgagatgca tcacgcttct cgcggtgtct
     3301 gggcgttcaa aaagggcagt aatagcagtg cggaaatccc ggcggcgatg gcgataacca
     3361 caaaaaatcc gctccagtgc caggtatcga gtactttcgc cagcggccaa ccagcaagcg
     3421 acgcaccaag ataagcaaac aagccgacaa accccgtcgc cgcccctgcc gcctctttgt
     3481 gggaacactc tgccgccgcc atgccgatta acatctgcgg gccaaagaca aaaaaaccaa
     3541 tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc cacagcgagc
     3601 cgactgaaag caaaattccg gcggcgaaaa tcaaattcat cggcccgcgg ttgccgttaa
     3661 acagtttgtc cgaaccccaa cccgctacca gcgcaccgat aaatccgccc agttcaaaca
     3721 ttgtcactgc cgtattcgcc gtgaccagat cgacgcccag cgtctcggac atatacaaat
     3781 tgccccagtc gtttatcgcc gcccggacca catagaccag cacatagcaa aacgaaagca
     3841 gccagatata cggattcagc aacacatatt tggtgaggat ctctttacgc gtcagccctg
     3901 ccccttcttg ttgttgagca atctccagtg cgtcgtgccg ccattcacca accgccggta
     3961 aacctaacgc ctgcgggcga tcgcgcagcc gccagcagag aaaaatcccc acgactatcg
     4021 ccatacaacc cgcaatcatc atcccggcac gccagccgta atgcagcgcg ctcgctgcca
     4081 tcacaatggg aatgagtgcg ccgccgacgt tatgtgccgt gttccataat gcccaccaac
     4141 cgccgcgctc ggtacgagaa taccaggcgg ttaacagacg cgcacacacc ggtgaacccc
     4201 agccctggaa aaaggcgttc aacacccaaa gcacggcaaa cgcccatagc gacgtcgaga
     4261 agccaaacag aatgttgata atgcccgtgg caataagccc tatccccata aaataacggg
     4321 catttgagcg atcgctgaca atgccggaga caaacttcga cacgccgtag gtaatgtaaa
     4381 acagggtcgc taacaggcca atatcgctac ggctgagcac gccgttagca aggatttctg
     4441 gtacggcggc gttaaaactt ttacgcgtga agtaaaacag cgcgtaaccc agccagatgg
     4501 tcagcagaat atgccgacgc cagtagcgat agcgggcatc aatttcatgt ttatcagtca
     4561 ttaatggcgc atcggcaggc gctttcagaa acggcaacat catcgaacct tagacataac
     4621 gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca ggaaatggtc aatgtgccac
     4681 ccagcgccgt tacgcgctcg cgcattccgg taaggccaaa accgtgttgc ccggaatctg
     4741 gcggtaaacc gctgccatcg tcttcaatca ccagcattaa ccgctcatca tgctgccagc
     4801 cttgcagcgt gaccgcgctg gcatcggcat gtttcacaat gttgttcaac ccttcctggc
     4861 agacgcgaaa cagcgtcacg cgctggtttt cgcttaacgc tgattcatcg attcgccatt
     4921 cgagatggct gacaatgccg cgcccttcca gctccatttc ccgcatcagt gagcggatgg
     4981 cctgctccag ggtgagatca tccagctggc gcggacgtaa ccgccctaac aagcggcgca
     5041 ccgcgtcgta aacgcccaac gacaactgtt cgatgagctg cccgctctgt ttcacgctgg
     5101 cgttatccgc cgcgagccgc tggacaatgc ccgcttgagt tcgaatagca gtaatggtct
     5161 gaccgatatc atcgtgcagc tcacgtgcca catcacggcg cacgctctct tcggtttcca
     5221 gcaaccgttc agcgagatgc tgattgcgcg ccagttcctt ttgcagcgac tggttaagtt
     5281 cacgcaaccg ctggatgcca gcgcccagta acaaccctgt cagactttgc accagcagcg
     5341 agagcaataa atccaccgga tgatcgcgcc aggtttgact ggcgatcagc gcgatggcgt
     5401 tcatcaatgt cgcaatcagc gctccttgcc aaccatagtg ccaggccagc gcgataatcg
     5461 gcagcgccag acagaatggc gtaaagcgcg acagttcgtc cggcaatccc aactggagcc
     5521 agagactgat aacaaacagc agcaagtacc agaccagatg ccgcccacgc cagttgattg
     5581 gttgagaaac cagtgacggg ccgagcggca gccaggtgtt attggcgaga tagtgccaga
     5641 acaccagaca tatcggggcc agcgtcaggc cgccagttaa agtcagcagt agcgcattcc
     5701 acgactcttt gccgtgccaa agccagggca gcgactgcaa caacgccgcc gccgttaatg
     5761 ccgcaccctg taacagcaag gtgcgccagt cacgttgatg gcgatagcgc gagatcagcg
     5821 ccacgggcag taacgtcagt aaactaccga tcatcaataa ggagaaatgg gttaaaccga
     5881 ccgcctgcgt taaccagtaa atcagcagcc actccgcgcc cagcaacacc ggccagtatc
     5941 cgcgcgggca ttgcagcatt agccctagcc gcaggccaaa cggaaataac agtaccgcca
     6001 tatcagggcg ctcaaccagg tgcaggctga tactccacag gcaaaaccat gcggcagaga
     6061 agataaaaaa gcaggcaata acggtaatta agcgggagaa caacgtcttc atcaccagcc
     6121 atcaaacatg cggcgcgcca gttcaacgtc gttactgacg cccagttttt ccatcaggtt
     6181 ggcgcgatgg acatgtaccg ttttcggtga caagcccagt tcggcagcaa tctctttcac
     6241 cgccattcct tgcgccagtt tttccgccac ctggcgttcg cgtttggtta gcgggtcctg
     6301 gcgaccggat gccagtttaa tggcaatatc cggcgtcaga taacagccgc ccgtggcaac
     6361 cgtatgcacc gcagcaatga gttcatccgg gctacagcgt ttggagagaa agccgcgcgc
     6421 ccccgcgtta agcgcctgct caaccagcgc cggactgtca tgaacggaga gcataattgt
     6481 cgccatgcct ttcggcagct ggcttagaag ctccagaccg gagatatcag gcatggagat
     6541 atcgcaaata cacacctgca caccgcgccc cggcagcccc gccagcgcct cgcgccccga
     6601 accaaactcg gcaactacct gcaaatcagg ttccagcccc agcagctgcg caaagccgga
     6661 gcggacgata aggtgatcgt ctataagggc aacggtgatc atgatcttgt cctggcgggt
     6721 aataaaacgc gcttacctta acgataagcg cgatgttgtt caagccttga gcggttactg
     6781 aaaaaacacc gcaatcttgt taaacatggt cggatcggac tgattacgct gcactttcac
     6841 cacatcttcc agcttgtcga tttggcttat catctgctcc agacgctggt cgtcattgac
     6901 cagtagccag atgtggcttt tgtcgctgtc ctgaatcggc aggcagagaa tgccttcaac
     6961 gttaaaagcg cgacgggcaa aaaggccaca aacgtgggtc attacacccg gatggttgcg
     7021 aacggtgagt tccagaatca cgttatcatg agttgtgttt tgcatggctt attcccccac
     7081 catttcagta ttcgccgcac ctggcggcac catcgggtac actttttctt cggcatcaat
     7141 gcgcacatgg atcagcgccg ggccagggcg attgatgatt tcctgcaatg cagcctgcgg
     7201 atcggcttcg ttattcaaat cacaggtttc gaggccgaat ccggcggcaa tctgcataaa
     7261 gttgattttg cccggatagg tagcggcaaa aacgccctgc ttgtagaaca gactctgttg
     7321 ctgatgcacc agccccagcg cttcgttgtt catcaaaatg attttaacat ccagctgatt
     7381 ttcactggcg gtcgccatct cctgaatatt catcatcagg ctgccgtcgc cggagaagca
     7441 caacactttg cggtccgggt tcgccagcgc cgcgccaatc gccgcaggca ggccaaaacc
     7501 catcgtgccc agcccgccgg aggtcagcca ctggcgtggg cgattgagcg gataggcttg
     7561 cgcggtccac atctgatgct gacccacgtc ggtggtgata atcgcattgt catcgacaca
     7621 ggcggcaacg gcgttgatca ggccgtaatg gcttaatgga tcgcacgctt tcgggattgg
     7681 acacgggaat tcacgctgca aatccgccac caactggtgc cactctgcac gcggttgcgc
     7741 ttccaccagc gggatcaact gcgccaacac gtcatcaaca tccgcctgaa ttgccacatg
     7801 cggttgcttg attttgccca gctctgcgcg gtcgatatcg acatgaataa ttttggcatt
     7861 cggacagaac tgctcggttt tgccaatcgc ccggtcatca aaacgcgcac cgagaacaat
     7921 caacaaatcc gcctcctgca aaatatagtt ggtgctgcgc acgccgtgca tccccagcat
     7981 acccagcgac agcggatgcg cttttggcaa catacccagc gccattaaag tcatggtggt
     8041 aggcagttgc gctttctccg ccagttcacg cactcgcgca ggcgcgttga tcacaccacc
     8101 acccagataa agcaccgggc gtttggcggc gttaatcatc gccgccgcgt cacgaatact
     8161 ttcttcgcta aaggcgggtg cggcggcttt ttctgccata gcgggctgtg tttcaatctc
     8221 aaaaactgcc gtttgcacat ccttaggaat gtctatccac accgggccag ggcggcctga
     8281 ttgcgcaata cggaaggcat cgctcatgac ctgcgggagt tcttcgatat gtctgaccag
     8341 atagttgtgt ttggtgatgg ggatagagat gccgtaggtg tcgacttcct ggaaggcgtc
     8401 ggtgccgatc atcgaggcgg gaacctgacc agtgatgcaa atcagcggga tggagtccag
     8461 cctcgcatcg gcaatggcgg tcaccaggtt agtcgcaccc ggtccactac aggccataca
     8521 gaccgccggt ttaccgtcgg tgcgcgccat tccctgagcg ataaaccccg cgccctgttc
     8581 atggcgagcc agaatatggc ggatttgcgt gctttggctt aaggcatcgt aaacaggcag
     8641 gatagaaccg cccggaatac ccgtcacaat cttaatgccc tgctgttcca ggaaatgaac
     8701 gataaattct gcgccggtaa agcgcttacg cgtcgatgtt gtgcccgaac ttgccatgct
     8761 ccagtccttt tcttctgggc cgactttccg ggaggagctt aaacgaaaaa ccccgcccgg
     8821 tttgcgccgg cggggttttg gaatcgtgtg ttgttccagt ccctacggcg cattgccgac
     8881 gaccaccacc acacgcacga cgaccactgc ggcggatggc gcagttggta gtagttttgc
     8941 gttgaacatg gaagtagtca ttggggacct tgttggtttt gtgtttaaca atatttatac
     9001 aagcacagct ttacagggga gacaatggaa aatttttctt ccggataaaa atacgtggca
     9061 gatcacattt tgaactgaaa ctcaggcaat aaagcatcat cacctgaaag acggtcagat
     9121 tgttgacaga gtgcgcctta ttcatgtcga atacagcgta aacaccttac cctaccaacc
     9181 gcaccatcac tgtttccggc aacatttatt gccgcttttg tttacatatt ctgccgctaa
     9241 acaattcccc attcctggcg tatatctggc taacattcat caatgtgata gattcctctc
     9301 ccgcatttat gggaatgcgt agtgacttat tctaattatt tttataaaag catccgtgat
     9361 aatgaaaagg catagaaacg tcaatttgtt attgatgttg gtattactcg tggccgtcgg
     9421 tcagatggcg caaaccattt atattccagc tattgccgat atggcgcgcg atctcaatgt
     9481 ccgtgaaggg gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg gtgtctcaca
     9541 gctgttttat ggcccgattt ccgaccgcgt gggtcgccga ccggtgatcc tcgtcggaat
     9601 gtccattttt atgctggcaa cgctggtcgc ggtcacgacc tccagtttga tggtattgat
     9661 tgccgccagc gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg cgcgtacttt
     9721 gccgcgcgat ttatatgaac ggacacagtt gcgccacgct aacagcctgt taaacatggg
     9781 gattcttgtc agtccgctgc tcgcaccgct aatcggcggt ctgctggata cgatgtggaa
     9841 ctggcgtgcc tgttatctct ttttgttggt tctttgtgcc ggtgtgactt tcagtatggc
     9901 ccgctggatg ccggaaacgc gtccggtcga cgcaccgcgc acgcgcctgc ttaccagtta
     9961 taaaacgctt ttcggtaaca gcggttttaa ctgttatttg ctgatgctga ttggcggtct
    10021 ggccgggatt gccgcatttg aagcctgctc cggcgtgctg atgggcgcgg tgttagggct
    10081 gagcagtatg acggtcagta ttttgtttat tctgccgatt ccggcggcat tttttggcgc
    10141 atggtttgcc ggacgtccca ataagcgctt ctcaacgttg atgtggcagt cggttatctg
    10201 ttgcctgctg gctggcttgc tgatgtggat ccccgactgg tttggcgtga tgaatgtctg
    10261 gacgctgctc attcccgccg cgctgttctt tttcggtgcc gggatgctgt ttccactggc
    10321 taccagcggc gcgatggagc cgttcccctt cctggcgggc accgctggcg cgctggtcgg
    10381 cggtctgcaa aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt tgccgcaaac
    10441 cggtcagggc agcctgggat tgttgatgac cttaatggga ttgttgatcg tgctgtgctg
    10501 gctaccgctg gcgacgcgga tgtcgcatca ggggcagcct gtttaagctc acgtcaccgc
    10561 agcatcgtca tcagctccat gggagaacga tgctgcttta tcagatcacg catcacccgc
    10621 atatgcggcg cggagtaaga ataaaacgcc tgatagcccg cacaaagcac gttgtctggc
    10681 gtctcctccc gagcgtgcgg gcacaggcga tgccagtcgc acgcctggca gccacgacgc
    10741 ggttgcgggt tttcgctgcc acaccatttt tgtagggtgg cgtcaaaaag ctcaatcgaa
    10801 atacgactga tatctgcccg tacccaacac tcgaacaggg caattaaaaa tttcccccac
    10861 agctctgctg tcaccgaagc ggccgtcaga tcgccccggt catcaaattc aagatgggga
    10921 ataagctgta cggagcggat accccgttgt ttgaaattgt gataaaaatc ggcaggttgc
    10981 agagcatcct tgtactctac aaccagttta tgcctgtcct cttcagcatc aatgacctga
    11041 cttcctgtca tgcgactctc tcaataaata cgataagttg atgtatatgc gttacagcaa
    11101 acagtattgc gagagataac accgcgaggg agatcaaaaa tttgtcacgg actacgtgaa
    11161 attgggaaaa atcgctattc gtgacatcca ttaaattgcg attacgggtg tagtgccaga
    11221 ggatcagcgc cacaatggcg agaatgccaa tcgaaatcca gaataacatg cccgcctggt
    11281 gccagttgtg tttgatagcc aacgccatca acgcgccgta gcccagcatg gtacgaaacc
    11341 acgccagtga tgtacgctcc ggctgcagcc ctgggtcggc aatgcgtctg gctttgcggc
    11401 tatccggcat acaacaccag tcccataacg atcactgcca cgaccatcaa tattaaactg
    11461 atgattaata agctgttggt atagggcagg tcctctttca ggcgcatcgc tttttcattg
    11521 cgtaaccagc gcaagtagcc atacatcgct aacccgccgg aaaacagaca caacaacaac
    11581 gccagcagtt cgcggatgac gggcgtggcg aaatctggcg caagttgatc aagcccaacc
    11641 cccgccgcca gaaatcctag tgcagtacgg atccacgcca gaaaagtacg ctcattagcc
    11701 agcgagaagc ggtaatcggg cgcttctccg aggcgggaaa ttttcattgt tgctccttgt
    11761 aaactgttcc tcgccgaaaa attaggccag gaacagcaat aatgcatcag aacttctgtt
    11821 ttttctcttc caccttcacc ccttgtgtcg gtaaaccggt gtcatagtcg cgtaccactg
    11881 gagaataacc atcctgtggg cgtggacgaa acgcccccat ccagcgcggt tgtgcatctt
    11941 tgcgccacgg acgcaggttc cattgataac tgcggaacgg atcgcgaatt ttgtccatgt
    12001 aatccaataa ggcgtcatgc attttgctgc gaacgtcggc aaaatggata tcatcgatca
    12061 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    12121 tgagcaccag tttaaagtca tccgttaccc agcaacgcac cggaataaaa ccgccaaagc
    12181 tgtcatgctc aatctcgtag cgattaaatt ccaccaacac gccacgtggt tcttttacgg
    12241 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    12301 tcggcagtaa gtcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    12361 aacggatgat cagcggaatg cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    12421 gcttatgtgc gcccatcatt tcgccgtgat cggaggtata aataacccac gtattttcac
    12481 gttgctctgg cgttaaggca ttgatgaccc gtccgatttg gtcatcaaca aagtcattgc
    12541 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    12601 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctctgct ttctcaccca
    12661 gttcgtagta aaaatcagtg tatttctcta aatactccac cggacaggtg aacgggtgat
    12721 gcggctcatc atacgaaatc accatcagga aaggttcgtc ggcgcgcgcg ggctgttgca
    12781 gaaaacccac cgcccgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    12841 tcgcctgtaa atcctcaacg ctgtttaggc cattgcgcca caggctaatc tctttctccg
    12901 tcagttcact aagatagttc gcgccatcga accagtaatc agcgtcccac tccggcggac
    12961 actcgccagt gccgaaatag tcatgtccat cgagatgcca tttgccgatg tagcaggtgt
    13021 gatagcccgc atccttaaag tagcgcccca tggtggaaat gtttttgccc ggcgcgacgt
    13081 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcgcgtgcag
    13141 gtgtacaaac cggtgaacag gtgtaggcag aattaaagcg aataccttct gccgccagac
    13201 tatcaatatt ttgcgtattc agcggcttac cgctatagca accgaccata ttggtggcct
    13261 aggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct tcgcatcggg
    13321 agtaaaggtt acggctgggt cgtgacgatg ccgccagctg tcaaaaatca ggtagacaat
    13381 cactacggcg gcgatgaaat aactgattat cgctaaccag cgcgtaccgt agccaccgaa
    13441 ttcagccagc ccggcataga cgccaatcat cgcgaacagg ataccaattg acgcaatctt
    13501 gacgtttttc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga acggcgtggc
    13561 gcgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga acgtacaggc
    13621 cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga tgtagctgat
    13681 tatgccaatc cccatcgcca cttttgccgc cagcgccgga atgcgcggga agaaaaagcc
    13741 cataatgatg atggtaacca gcggcacgtt gtaaataccg ttgagctgtt tcatccagct
    13801 atacagcccc tgcggcgcgt tggcgatcca cggcgcgacc atcaccgaaa cgatggcgat
    13861 aaagaaacca aatttgcgcc cgacggtgac cagctgttgc ggctcggcat tctggttaat
    13921 gatgcgccgg taaataccca tactgaataa cgtactggcg ctattcagaa agccgttgaa
    13981 ggtactgatc accgcaccaa ataacaccgc accgaagaag cccaccagtg gcaccggcag
    14041 aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtaaatcct gatacagatg
    14101 aaacgcaatc aaccctggca gcaccagtac cagcgggtcg agcattttca gcaccgccgt
    14161 taacagcgcc cccttttgcc cttccgctag gctttttgac gccagcgtgc gttgcacgat
    14221 gccctgattt gtacaccagt aaaaggtgtt caccagaatc aaaccggtaa atgccgcgcc
    14281 aatcggcaag ggatcggtcg ggccgccgac tgagtttaat ttctcggcat gaacggtggt
    14341 gagttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaggc caaataccgg
    14401 caccatcaac ccgccaataa ccagcccaat accgttaatg gagtccgcca ctgccattgc
    14461 gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa taaccagtaa
    14521 ccagatagcc gcaccatcgg aaagttgtag cgactcaccc acatgaaaca ggctgttgag
    14581 cgccaacgcg ccggaataga gaacaatcgg cagaaagcag acgccggtgg caatgaggaa
    14641 gcagaagtcg atgataatac gcgtcgtttt atcataacgt tcctccagaa aatcggggat
    14701 ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga agatcagcgt
    14761 cactgcagag gtgacttccc agcccatcac cgacatgccg cttttgtagg cctgcccgga
    14821 aaggccgacc agttgttccg tggaaaggtt ggttagcatt aacgaagcgg caataaccgg
    14881 cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat ctgttttacg
    14941 gaccttccac caggtgatga ccgctaccag cagcgtaaaa ccgacaaaac tcaagatttg
    15001 taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg aatataggga
    15061 gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc gtatccctga
    15121 acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg atattaaaat
    15181 gaatggaaaa ttgcaaagtt cggatgtaaa aaacgaaact ccgtacaata ttcccttatt
    15241 gattaacgaa aatgtgatct ccagcggaat ttctctgatc tcgctctggc atacctacgc
    15301 cgacgagcat tatcgggtga tctggccgcg ggacaagaag aaaccgctta tcgccaactc
    15361 atgggttgcg gtgtataccg tacaaggatg tgggaaaatt cttttaaaga atgacgaaca
    15421 aataactctg catggcaact gtattatatt tttaaagcca atggatattc actcttatca
    15481 ctgtgaaggt ttggtctggg aacagtactg gatggaattt acccccacca gtatgatgga
    15541 tattcccgtc ggtcagcaaa gcgttattta taatggcgaa gtttataatc aggaactcac
    15601 cgaagttgct gagttaatta cttcaccaga agcaataaaa aataatctgg cagtcgcttt
    15661 tctgacgaaa attatttatc agtggatttg tcttatgtac gcagtcggta aaaaagatcc
    15721 acaacggcgg caaattgaaa aattaattgc cactttacat gccaatctac aacaacgctg
    15781 gagcgtagct gatatggctg ccacgatccc ctgtagcgag gcctggttgc gtcgtctgtt
    15841 tttacgctat accggcaaga cgccgaaaga atattacctc gatgcgcgtc tggatctggc
    15901 gctatcgcta ttaaagcaac aagggaactc ggttggcgaa gtcgctgata cgctcaactt
    15961 cttcgactcc tttcatttca gcaaagcctt taagcataaa tttggttatg cgccgtcagc
    16021 tgtgctgaag aatacggaca ggtaataacc agaatggcga gtgtgtaaat gactcgatga
    16081 tgttcagaaa taccaggcgg agtaccattt acggcgactc caccgggaaa agagttaacc
    16141 gatactccag aagagcaccg ccagtaattg gggtgtgata atgcgcagga acatcactaa
    16201 cggatagaca gtggcgtaag agagcgccgc cgcaccgctg gttggatgaa gattattagc
    16261 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    16321 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    16381 cagggcacca taaccaatcc agcttagccc ttcgccatcc accagtgtat ggataaaatc
    16441 gccgccggat ttcaggccaa ccaccgagag aaagagcaca atgcctaact cgcgtagcgc
    16501 caggttagcg cttggtggca taaaccagta cagcttgccg atactgccga tacgcccgag
    16561 gatcaacgcc ataatcagcg gaccgcccgc cagcccgagt ttcaacgccg ccgggaaccc
    16621 aggcacaaag acgggaatag aacctaacaa tacgccaagc ccgataccaa taaacactgg
    16681 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    16741 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgct
    16801 acttgcaact aattcaacac cagcacggtt caggcgcgag ataacaacgt catagcgttc
    16861 tttaaagtgc aggtcgcgaa tacgtttacc gagcacgttt tcattggtga ccaccacacg
    16921 cgcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    16981 cagttgcgca ttatgtaaat ccgcaggctg ccccaccaga tgcagcaaat cgcccagttg
    17041 aataacggtt tctggcgaag gcacttttag ggtttcttct cgtttcaatc gtgagcaaat
    17101 aactttatcg ccattgagaa tcggcacatc tttaatggct aaattatgca aattaggatt
    17161 ctcaactcgg atgttaatcg tcctgattag cgcaccgccg ttggttcgcg tggactcgtg
    17221 ctgctgggcc tcagtctcaa cattgacgcg gaaaataacc cgcagcatcc acatagtgaa
    17281 caaaatgccg caaatcccaa atggatacgc catcgcatag ctcatcccca tttgatcgac
    17341 catcgccatc ggtgtcccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    17401 attggtgacc gcgccggaaa aaatccccag cactaccggc agtggaatat caaacagttt
    17461 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    17521 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    17581 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    17641 catccccgcc tgagaaacaa aatggccgac gatgatccca ccgaacagca cgccgccaat
    17701 gcccaatcca acaccgcgaa acttgacgtt gccgataaac aacccgacga ccgccaccaa
    17761 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gaaataacat
    17821 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    17881 tcacgggaaa gaacgtgccg aaatatctta aacaggcgaa atttaacgca ttgctgaaga
    17941 aaaaagacaa aaaaaagccc caccagaatg gcggggcaaa gagaatagct agttagctat
    18001 ttaacgcggg acgttcgctg atagcgatac gctgcgctgc gatgggttca ggctcattac
    18061 gaattaaatc aatatgcagt aaaccgttta cgaaggttgc gccagagact tccatatttt
    18121 cagccagcgt aaagctcagg ctaaaaggct gattcataag cccttgatgc agccattttt
    18181 tctcttcttt tggctgctct ggcgtgcctt ttacgctcag gcgcgtacct tccagttgaa
    18241 tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag tggttatcgt
    18301 cgcttttctc aatgttgtac ggcgggaagc tctggctttc accggcgttt tgcagtgcgt
    18361 tggccagttt gtcaaaaccg atccattgac gcatcagtgg ggataaatcg aagttacgca
    18421 tagccatttc tccttctaag aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc
    18481 tccctgacgg cgagcatgga aatgtcaggc cgcgccaggc ggccttaggg aattagttga
    18541 tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg atatacagca
    18601 gaccattcac caggttagca ccacgaacat gaatgttctc agctaactgg aatttacgtt
    18661 caaagttgcg ttcagcgatg ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt
    18721 gagcaccttt caccaccagc agattatcct gggcggtaat ttccagttcg ctctcagcaa
    18781 aaccagccac agcgatagca atgcgataat ggttttcgtc taccagttca acgttatacg
    18841 gagggtagcc gccattactc tggctctggt tgttttctaa gtggttaaac aaacggtcaa
    18901 atccaatagc agaacggtaa agcggggata aatcaaagtt acgcataatc aatagctcct
    18961 gaaatcagcg agaatctaag accttccaca atggacaggt caggtagcca gaacacccat
    19021 caggcgcgtc ctcatcggct acgatgtaaa aatgggtctg gaaatgaact tttcaagcct
    19081 tatcagcgga cttttttttg cagtttatgg tctattgcat agactgaggg ggcagcacga
    19141 aacgttaaag tgcgatagcc gccacagtgc gacgtaatga tgatgttatt ttcagcaagg
    19201 gatcgctata actcatcagg ccaacaccca cgatcaacag atgaatttat tatgataaga
    19261 aatgtgttgt tagcgttcat gatatgcagc ggaatgacat tactcggagg atgctccagc
    19321 gtgatgtccc ataccggggg gaaagaagga acgtatcctg gcacccgcgc cagcgcaacc
    19381 atgattggcg acgatgaaac caactggggc acaaaatcgc tggcgatact cgacatgcca
    19441 tttaccgcag tactggacac aatattgcta ccgtgggatg tgttccgcaa agacagctca
    19501 gtgcgctcgc gcgttgaaaa aagcgaagcc aacgcgcaag aaaccaataa cgtcattccc
    19561 cccgccaaaa tgcctgcaaa ctgagagcta gcggtcagtt tcggtgatcc acaactgacc
    19621 gtcctccatc cacgccaacc attgaccatc cggcgagaaa accacggcgt ccgcagaagg
    19681 tggattggcg tggttttcgg ttaaatactc aacctcgccg ctttgcgcat gggcacaagc
    19741 aatccgatta tccagcacaa atcccagcca ttcgcctgat ggatgccagt taaatgcaga
    19801 ctgaatatcc gttttattat gggttaactg gcgtggttca ccgccctgtg gcgagataag
    19861 ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcaatttgtg ttccttgagg
    19921 attacaacgc acccagtggc gcggaatgtt aaccaggccc gggtaaatac gatggtgcgt
    19981 aaaggttaaa cgtcgctgca cgacaccacg cggtggcgca ggcagggttg tttccgttcc
    20041 gcttaacggc gcatcacctg ccactttcca gccagcttca tctttcggca actcgacaat
    20101 aaacagctcc ggcactttct cgccctttgg cgaaagtgtg tcgccaataa acgccagtgc
    20161 gtgatttcct acccatcctt cttcataagc acgattgatt tcatcactgc caggctgtgg
    20221 cgtgggcgtg gttttactta ccagcacgca ccagtggcta ccgctgtatt cacgcggatg
    20281 ctgcttttgt acgttgaccg ggccaaacgg tgcagcaaca ccgacgtttc gcaaatccag
    20341 cgccggatcg agttcatgca ttacatggtc gttgtaggta aagctcaccc tttcaccgtt
    20401 cgggctaaag acatgtacat gactgccgcc gcgcagcgct cctggagtat atggcgcggt
    20461 aatatccatt gcatcgagat tgctcacctt gccgctttca acaatcactc cgcgccgatg
    20521 atgaaagtcg taataccatg tttcatcagg attttccggg ccgtggatga agacatactt
    20581 ctctgacttc gtatgaacgg tcaccacgcc gacgtatgcg ccctgtgacg cgcgatagat
    20641 aacctcgacc tcgccggtat gaatattcac acgctcaatg gtctcgccgg taaacgacgc
    20701 cccagaaggc cgcacgtcaa acaccagcca ctggctgtcg ggcgtccagg tattggtatt
    20761 ggtgagcagg tgattacggg gagcaaaggt tatctgtttc atggcgcggt cctgatacaa
    20821 aacggttccc gccatcatac ttcacaagga cttcactttc agacaatagt cttttgccat
    20881 cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg gcgattattg gcctcggccc
    20941 ggcgggtcgg cgctggcgcg gcagttagca ggtaaaatgc aggtgatcgc gctggataaa
    21001 aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct ggcaccggac
    21061 gcgcagcgat cttttattcg cgatggactg acgcttcctg tcgatgtcat cgccaacccg
    21121 cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa ctaccagcga
    21181 agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct gatccccgcc
    21241 agcgttgagg tttaccacga cagcctgtgt cgaaaaatct ggcgtgagga tgataaatgg
    21301 catgtcattt ttcgcgcaga cggctgggag cagcatatta ccgcccgcta tctggtcggt
    21361 gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca gattcgtaaa
    21421 tatgtcgcca tccagcagtg gttcgcagag aaacatccgg tgccgttcta ctcctgcatc
    21481 tttgataatg cgataactga ctgttactca tggagtatca gcaaagacgg ttattttatc
    21541 tttggcggtg cctatccgat gaaagacggt cagacgcgtt tcacgacgct aaaagagaaa
    21601 atgagcgcct ttcagttcca gtttggtaag gcggtgaaaa gcgaaaaatg cacggtgctg
    21661 tttccctcac gctggcagga ttttgtctgc ggcaagacaa cgcctttctg attggcgaag
    21721 cggcgggatt tatcagcgcc agctcgctgg aggggattag ctatgcgctg gatagcgcag
    21781 agattctgcg ttcggtatta ctgaagcagc cagagaagct caataccgct tacaggcgcg
    21841 ccactcgcaa actgcgttta aaactcttcg gcaagatagt aaaaagccga tgcctgaccg
    21901 caccggcttt aagaaagtgg attatgcgca gtggagtggc gcatattcca cagttgaaag
    21961 attatccaac gcgcttcaca tcgcccacca gcaggatgta agagagcgcg ccaatcaacg
    22021 cgacggcgga gatataaacc agtgcaggcg cgaaaccgta accctgcgcc aggtatccca
    22081 ccaccagcgg aacggtgatg ccgcccagac caccggcaaa gttaaacacg ccgccggtta
    22141 aaccaatcag gcgcatcggt gccagagaag agaccagcga ccaggttatc gaagcaaaac
    22201 cgttgccgaa gaatgccagc gccatcaggc acataatcat catcggatcg ttggtgtagt
    22261 tcgcacccat aatgcaggtg gagatcagca agccgcagat aatcggcgtt ttacgcgcaa
    22321 agcccagtga aaagccctta cgtaccagca gatccgcgac ccagccagag agcaggacgc
    22381 cgacaaacgc cgcgaggaat ggcaccgtgg tcataaaacc tgctttcagc gccgtgattc
    22441 ctttttcctg ggttaaatag ttcgggaacc aggttaagaa aaaccacagt gtagaagcca
    22501 ccgcaaattg cccaagatag acaccgatca gtttacgatg gaacaccagt ttccagtctt
    22561 tggctgttaa cggctgacgc gcctctttct tcaccggcgc atcgccatcc accagaccgc
    22621 cgccatcacg aatgtaatcc agttcagctt tgctgatgcc tttggtcaga cgcggcggct
    22681 gataaacctt aaaccaaatc agcgaccaga taatgccgat accaccggtg acaatgaaca
    22741 cccagtgcca gctcaacatc tcctgaatcc agatcagcag cggagtcaga aacgccagac
    22801 cgacaaactg accagacgta taaaaaccaa cggcagaagc gcgttcatgt tccgggaacc
    22861 aactggttac catccggtta ttggtcggga acgcgggcgc ttcgaaaata ccggttatcg
    22921 cgcgcagacc aattaatgac attaagcccg tggcaaagcc ctggaataac gtcgccactg
    22981 accagccaaa tatcgcaata aaataagtca cgcgtgaacc tacgcgatct aaaaaccaac
    23041 caccggggat ctgacatagc gtataaagcc aggcgaaggc cgaaaataca tagcccattt
    23101 gcgctttagt aataccaaac tcttcctgaa tatgggcaga agccacggcc aggttagcgc
    23161 ggtcaacata acaaatgacc accgtaataa agatcatcac cagcgtcaga taacgccgac
    23221 gccccggctt tgctgcatta acgggaatat ccatagcgag ctttctccag attttgggca
    23281 tagcgaagcc gctcaccatg ccctgtaatt tacagagggt tatatttttg tattgctgtt
    23341 ttagtgcccg atgaggggct tacgtggcag gaattaccac tctgctacgc tgttatcttc
    23401 atgacgccag agcggattac gccagtccgg ggcattttta ctgaactcaa taactttagc
    23461 ttcgtcgatt tccacgccta agcccggttt cgttaatggt ttaaagaaac cgccgaccat
    23521 gctgaagtcc tctttgtttt tcacaaagtc gagtaactcc gcgcctttgt tgtaatgaat
    23581 gcccatactt tgttcctgaa ggacggcgtt ataggaaaca aagtcgatat gcaggcaagc
    23641 cgccagtgca atcggtccga gcggacagtg cggcgcaagg gtcacgtcat aggcttctgc
    23701 cattccggca attttgtagc attcggtaat accgcctgcg tgggagagat ccggttgcag
    23761 aatcgaaata ccgcctgcct ccagcacacg tttaaaatcg aagcgtgaga acatgcgctc
    23821 acccgccgcc agtggaatat gcgtttgtgc cgccagtttc gggtagtatt cggcctgttc
    23881 cgccagcacc ggctcttcaa taaacagcgg acgatacggt tccagctctt taatcagcac
    23941 tttcgccatc ggcgcgctga cgcgaccatg gaaatcaaga ccaaactcaa tctgattgcc
    24001 aaaagcttca cgaatttgtg ccacggtgtt gactgccgca tctaccgcgc gggagttatc
    24061 aattagcccc agttcttcac aaccgttcag tttgaaggta tcgaagccga tttcgcgtag
    24121 cgttttaatg ccgtcgataa catccgccgg acgatcgccg ccgacccaac tgtaggcttt
    24181 aattttgtcg cgaaccaggc cgcccatcag ttgccagacc ggcgcattca gcactttacc
    24241 tttgatatcc cataacgcct ggtcgatccc ggcgatggcg ctcatcagga ttggaccgcc
    24301 acgataaaat ccggcgcgat acatcacttg ccataagtcg ttgatgcgtg acgggtcctg
    24361 accaatcaaa tagtcactca gctcgtggac tgccgcttcc accgtgcgag cgcggccttc
    24421 aatcacgggc tcgccccaac cgaccacacc ttcatcggtt tcaattttca ggaacatcca
    24481 gcggggaggt aaccgatacg tggtaatttt ggtgattttc attgcactgc ctctcgatac
    24541 gccttaacaa atgctgctgc ctgctgcgca gtccgttcta cggactgccc ggcgcgatag
    24601 agatcactgc ctaagcccgc ccctgcacaa cctgcgtcta tccactgcgc caggttttct
    24661 ggcgtcacgc cgccaacggc aaagactgcg atgtccggtg gtaataccgc ttttaatgct
    24721 ttgatgtatt gcggaccaaa agccgatgac ggaaatattt tcagcgcctg cgcgcccgct
    24781 tcgagcgcgg taaaggcttc ggtcgccgtc gcacagccgg ggcagacggt catgccgtag
    24841 cccaccgcac ggcggatcac ttcactatgg atattgggcg taacgatgag ctgacagccc
    24901 attctggcga gcgcatcgac ctgttcaggt ttcagtaccg tacctgcgcc aatcaacgcc
    24961 ttatcgccgt acgcatcaac gatggcggga atgctctgct cccattgtgg agaattcagc
    25021 gggatttcaa ccgcatcgaa cccggcgtca atcaccgcgc caacatgtgc cagcgcctcg
    25081 tcgggcgtaa tgccgcgcaa aatggcgatc agcgggagtt tagtttgcca ctgcatgagc
    25141 gatgctcctt ataccagcct gaaatgccgt gtcgcctgcc accgccgtca cgtcgcaacc
    25201 catcgcctga aaggcttgct ggtagcgcgc ggtcagcgat gttccggcga caagggtgat
    25261 ggcgtgttga tggtccacat agtcacgcat actggcaacc tctgcgccaa tcaacaaacc
    25321 agagagaaat tcgctgacct gttcgcgggg aagtgttccc agcacatgcg aggcgcgaac
    25381 ctcaaaaagc tgtggcaata cggcgggcgc attaaggcca tgctcaagac cagccgcgaa
    25441 ggcatcggca gcgttttcct gcggcggcaa acctgcgcca atcaatgagt gatttaacag
    25501 taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg ttaatttgct ggctatcggc
    25561 ctgcacccat ttgcaatgcg taccgggcat gacataaaga gaggaaggag ccagagtgcg
    25621 cgcgccgatc aattgtgttt cttcgccgcg catcacattg tggttatcgt catgagagac
    25681 gcataatccg ggaataatcc agatattgtc gccaacagac gttaattgtt cgccaataga
    25741 cgaaaaacgg gctggaacgg ataaatacgg cgcaactttc cagccgacat tgctgccgac
    25801 cattcctgcc attaccactg gcgtattctc ttcacgccag ccggtcgtca cttctgctaa
    25861 caccgcagcc ggagattttc cgttcaggcg cgtgacgcct gcttctgatt gcctgctgtc
    25921 caggcagtgg tcgccctgat aaagccaggc gcgcagattg gtcgatcccc agtcaattgc
    25981 gatgtagcga gctgtcatgt gatttccttt aaccttcgtg tcgagctggc gatcatggta
    26041 agcgccgcct gctctgccgc atcgccgtcc tgatgcctta tcgcatcgaa cagcgcctta
    26101 tgttcctgga gcgtttgcgg catgttggcc tcatcgccca tccaggttcg ttcaaaaacc
    26161 gcccgctgca gcgaactgat cgcaatgctc agttgctgta acaccgggtt atgcaccgac
    26221 tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa acgcttcgcg gtcctgattg
    26281 ttggcaatca tctcgttcag cgccgattca atctgcgcca gatcgctgga ggtcgcgcgc
    26341 tctgctgccc aacgggcaat agccggctct accaggtttc gcacttcgct cattgcactg
    26401 ataagccgtg ggtcatagtc gttttccagc acccattgca gtacgtcagt gtcgaggtaa
    26461 ttccactggt tacgcggagc cacaaacgcc ccgcgataac gtttcatttc aatcagccgc
    26521 ttcgccatca gcgaacgaaa tacctcacgg atgatgttgc gcgaggttgc aaactcttca
    26581 cagagttctg cctcagccgg aagcggcgag cctggcacgt atttgccgtg aacgatctgt
    26641 ttacctagcg taataacgat gcgatcggtt ttattgagag tcatggagag tccttgtgct
    26701 ctgtatgttc ttctctactt taccccgatc gatgcataac gcggcaattt tgtagtacca
    26761 gcgtgatgac gttcgcgttt gttgtgcgtc taatgtagta caaagttata ttgttgtact
    26821 acaatttaga tcacaaaaag aacaatcaat aagaaaatgt tctacgtcgg gcaaaaatct
    26881 gaaaagggag atcaggcgct aaacaggagg gaaagaagag tatgctttca acggcttagc
    26941 actcttgttt aaaggattaa tcatgaaatt gaattttaaa ggatttttta aggctgccgg
    27001 tttgtgttct ttggcatttg cgttgaccgg atgtatcacc tggggattgg tttccaatac
    27061 tgcaagccgt ccacatgccg agcaatggcg gtcagacacg attaagggtt tatcgctggc
    27121 agaagatagt aacggtacga aaggctatgt ttttgttggt gaatcgttgg actatctgtt
    27181 gaccaccggc ggcgatgagg tagttaacat gctgaacgat ccggcaatcc atggtgaaag
    27241 aatcaccgtt tctgataacg cgaaatttat tttaagcagc agtaataaga atttctctgg
    27301 tgctattacg ctctattacg actggaacaa cgaagaagat aaggcactgg caacacaata
    27361 tggttttatt tgtgataccc ggcgttgtac atggatgctg gacggtttga aaggctcaat
    27421 ccaccagaaa aataaaaaag cggattactc aaacgtgatg gttttccatc aaccctttac
    27481 ggtgggattt tatgagtaca aagcaacaga tggcgtcccg cacggcctcg ttaatgcgct
    27541 gctgccagtc actcttacgc tggatatagt aacgtcacca ctccagttcc tgattttatg
    27601 cacgaccaga aattgttaat cgctgcgggc aaacgcattc atgccgtttg cccgttcact
    27661 gattaattca gcacatactt ctcaatagca aatgccacgc catcttcaag gttagatttg
    27721 gtgacaaagt tcgccacttc tttcaccgaa ggaatagcgt tatccatcgc cacaccgacg
    27781 cctgcatatt caatcattgc gatgtcgttt tcctgatcgc caatcgccat gatttcttcc
    27841 ggtttaatac ctaacacgtc ggccagtgat ttcacacccg tacctttgtt aacgcgttta
    27901 tcgaggattt cgaggaagta cggcgcactt ttcagcacgg tgtatttttc tttcacttcc
    27961 tgcggaatac gcgcaatggc ctggtcgagg atggcgggtt catcaatcat catcactttc
    28021 aggaactggg tattggggtc cattttctcc gcttcgcaga acaccagcgg aatggtggca
    28081 acgaaagatt catgcaccgt gtagtagctg atatctcggt tggcggtgta cagcgtggtg
    28141 cggtccaggg cgtggaaatg agaaccgact tcgcgagaga gtttttccag gaaacggtag
    28201 tcgtcatagc tgagagcggt ttgcgccacg gtgctaccat cagcggcctt ctgtaccagc
    28261 gcgccgttat aagtaatgca gtagtcgccc ggctgttcca tatgcagctc tttcaggtaa
    28321 ttgtgaacac ctgcataagg gcgacccgtc gttagcacga cattcacgcc acgggcgcga
    28381 gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt gatcgggcag cagaagggtg
    28441 ccatccatat cgatagcaat gagtttgata gccatgagtt ccccaggtag attggttcct
    28501 gacccatgct aacgcgattc cgctcaaaaa tcagtacaac acccgaggga aaagggggat
    28561 gcaacgcgca ttgcgtgctc cccttttgct tagcggaaaa gtttcccttt cagcagttcc
    28621 atgcctgcgg aaagcagatc gttattggct tgtggtgaca cttcaccttg cggtgagagc
    28681 gcatcaataa tcttcggcaa ttgttctgcc agtaaactgg aagctgtact ggtatccacg
    28741 ccaagttttt gcccgagatc ggacaccgca tttgtgccga gcgccgattc cacttgctcg
    28801 ccgctaactg attgattgct ctgttgatta ctcaaccagg ttgagagaat ggcccctaag
    28861 ccgccacttt gcagtttttc cagcagcacc tgaatgccgc cctgctcctc aacccaactt
    28921 aaaatcgcct gatatttccc cgcatcgcct ttcagaaagg cgccgacaac gtcatcaaaa
    28981 aggcccatga taatcacctg taaagcgtta cgtgttgact caaaaagtat agttctgtgg
    29041 gatgataatt gcggattgca ggaataaaaa gggcggagat gatctccgcc cttttcttat
    29101 agcttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaaacat gcaaattcaa
    29161 tatattgcag gagtctcgta ggcctgataa gcgtagcgca tcaggcacgc tcgcattgtt
    29221 aacgccatta aatatcgata ttcgccgctt tcagagcgtt ctcttcaata aacgcacggc
    29281 gcggttcaac ggcgtcgccc ataagcgtag tgaacaactg gtcggcagca atcgcatctt
    29341 taacggtaac gcgcagcata cgacggcttt ccgggtccat ggtggtttcc cacagctgtt
    29401 ccgggttcat ctcgcccaga cctttataac gctggatgga gaggccgcga cgggactctt
    29461 tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg ctgacgacgt tcgccacgtt
    29521 cgataaatgc atcttcttcc agcaagccac gcagtttctc acccagcgtg cagatacgac
    29581 gatattcgcc gccagtgata aattcgtgat ccagcggata gtcagtatcc acaccgtggg
    29641 tacgcacgcg aacaatcggc tcgaacaagt tttgctcagc attggtgtgg acatcaaact
    29701 tccactggct gccgtgctgt tctttatcgt tcagttcgct gaccagcgcg ttcacccagc
    29761 gggtaacggt ctgctcgtca gaaaggtcgg tttccgtcag cgtcggctga tagataagct
    29821 ctttcagcat tgctttcgga taacgacgct ccatgcggtt gatcattttc tgcgtcgcgt
    29881 tgtactcaga caccagtttc tctaacgctt cgccagccag cgccggtgca ctggcgttgg
    29941 tgtgcagcgt tgcgccatcc agcgcgatag agatctggta ctgatccatc gcttcgtcgt
    30001 ctttaatgta ctgttcctgc ttacctttct tcactttgta cagcggcggc tgagcgatgt
    30061 agacgtgacc acgttcaacg atttccggca tctgacgata gaagaaggtc aacagcagcg
    30121 tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat gatgatgctg tgataacgca
    30181 gtttgtccgg gttgtactcg tcacgaccga taccacaacc aagcgcggtg ataagcgtcg
    30241 ccacttcctg agaagagagc atcttatcga agcgcgcttt ctcgacgttg aggattttac
    30301 ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc ctgcttcgca gagccgcccg
    30361 cggagtcccc ttccacaagg tacagttcgg aaagcgccgg atcgcgttcc tggcagtctg
    30421 ccagtttgcc cggcaggcca gctaaatcca gcgcaccttt acggcgggtc atttcacgcg
    30481 cgcgacgcgc agcttcacgg gcacgggcag catcgataat tttgccgacc acgattttcg
    30541 cgtcggttgg gttttccagc aggtattccg ccagcagttc gttcatctgc tgttcaaccg
    30601 ccgatttcac ctcagaagaa accagtttgt ctttggtctg tgaggagaat ttcgggtccg
    30661 gcactttcac ggaaacgacc gcaatcaggc cttcacgcgc atcgtcaccg gtggcgctga
    30721 ctttggcttt tttgctgtag ccttctttgt ccatgtaggc gttcagagta cgggtcatcg
    30781 ccgcacggaa acctgccagg tgagtaccgc cgtcacgctg cggaatgttg ttggtaaagc
    30841 agtagatgtt ttcctggaag ccatcgttcc actgcaacgc cacttcgacg ccaataccgt
    30901 ctttttcggt ggagaagtag aagatattcg ggtggatcgg cgttttgttc ttgttcagat
    30961 attcaacgaa cgccttgatg ccgccttcat agtggaagtg gtcttctttg ccgtcgcgct
    31021 tgtcgcgcag acgaatggaa acgccggagt tgaggaacga caactcacgc agacgtttcg
    31081 ccagaatttc atattcgaac tcggtcacat tggtgaaggt ttcaaggctt ggccagaaac
    31141 gcaccatggt gccggttttt tcagtctcgc cggtaaccgc cagcggggcc tgcggtacac
    31201 cgtgttcgta gatctgacgg tgaattttac cctcgcgctg gataaccagc tccagttttt
    31261 gcgacagggc gtttactacc gaaacaccaa cgccgtgcag accgccggac actttatagg
    31321 agttatcgtc aaatttaccg cctgcgtgca gaacggtcat gatcacttcc gccgccgata
    31381 cgccctcttc cgggtgaata ccggtcggaa tgccgcgccc atcatcctgt acggagacag
    31441 agttatcggc gtgaatggtg acgataattt ctttacagtg acccgcgagc gcttcgtcga
    31501 tagcgttatc taccacctcg aataccatgt ggtgcagacc ggtgccgtca tccgtgtcgc
    31561 cgatatacat acccgggcgc ttacgcaccg catccagccc tttcaggact ttgatactgg
    31621 aggagtcata agaattcgac atcaacgttt ctcgctcatt tatacttggg ttaatccgtt
    31681 attttaccct tttccacggt aaacatcttc gaattttcgt ccgacatgtc tataacgtgt
    31741 tcagcactga tcgcgctgac aaagacctgt gattgcgtcg cttttaagcg gctggcaagc
    31801 agcccgcgac gctcatcatc aagctcagag gcaaaatcat ctatcaggta gagacaccgc
    31861 cgcccgcttt cacgggtgag gaactctcct tgcgccagac gtaaggcgca catcagcagc
    31921 ttaagctgcc cacgcgataa ggtatcttcc accggcgcac cgtcggcgcg aatgcgtaag
    31981 tccgctttat gcgggccatg cgcggtgtag gttagctggc gatcgcgttc aaaattacgt
    32041 tccagcacct cagcatattc tgtctctttc tcccagccgc gctggaaaga gaaagtcaga
    32101 gaaaactcag ggagaaattg cttacaggta tcggccatat cagccgcgat accggcgcta
    32161 tactccgcgc gccaggtgct gatttgctcc gccagcggga tcagttcttt atcccacggg
    32221 cgtagctgtt cgtaacgagt cacctggcgc agcgccgcat tgcgctgctt gagcaatcgc
    32281 ttgagattgc tccaggcggt gaaaaatccg ggttcgttgt gaaagcatcc ccagtcgagg
    32341 aatgctcttc tgtatttggg gccgccgttg agtaaagtaa acccttctgg cgttatcagc
    32401 tgcattggca tcaggtgcgc cagctccgcg actttatgcc cgtctgtacc gtcaatacgg
    32461 actttgctgt cgccttgttt gtctttggtt aaaccaatcg ccgtctcgcg ctcttcgccc
    32521 tgtaatcgcc cgtggagaac aaacgcctcc tgctcgtggc gaatgacgcg accaatctgc
    32581 aaactgcgaa acgcccgacc gtggccgagc gtatagatgg cttccagcac gctggttttg
    32641 ccactgccgt tggcacctac cagaaagtta aagccgggag ataaggcgag atccgcggtt
    32701 tcaatgttgc ggaaatcgcg gatcaacaag cgggtgaggg acattacagt ctcattggca
    32761 tgacaacata agccgcgctc tggctggccg catcttcaat ctgcacgctg gaaaccgaat
    32821 cggtcagcat catgcggacg ttttcgcatt tcagtgcgtt gagaacatcc agcacatagc
    32881 tgacgttgaa gccgatttcc atctccgcac cgctataggt aacgtcgagg atctcttccg
    32941 cttcttcctg ttctgggttg ttggcggtga ttttcagctg gttttcgctg acatacagac
    33001 gcacgccgcg gaatttctcg ttagagagaa ttgccgcacg agcaaacgcc tgcttgagca
    33061 gatcacagcc agcttccaga tgtttgtccg ggttcttcgg cagaacgcga cggtaatccg
    33121 ggaagcgacc atccaccagt ttcgaggtga aaataaagtc gccaacgtgg gcgcgaatgt
    33181 tgttgctgcc aatctgcacg cgcagcggat tgtcgccgcc gtcgagcata cgcatcagtt
    33241 caatcacgcc tttacgcggc acgatcaccg aatggcttgg caaagattga ccaattggca
    33301 ttgaacagac tgccagacgg tggccgtcgg tcgccacggt gcgcagttct tcaccttcgg
    33361 tttcaaacag cataccgttt aagtaatagc gaacgtcctg atgcgccata gaaaactggg
    33421 tcgcttcaat cagacgcttc atcgtcgcct gcggcagggt aaattcgact tcgctctgcc
    33481 agtcatcgag gttcgggaaa tctgccgctg gcagggtaga cagcgaaaaa cggctacgcc
    33541 cggagcgcac cagcatccgc tcaccttcca gctgcacggc aatttccgcg ccttcaggca
    33601 gaccacggca gatatcaaag aatttgcgcg ccggaacggt cgtcgcccct ggttcgtgcg
    33661 gctgaaccag cgcaacgcgt gccaccattt ccatctcgag atcagtaccg gtcagcgaca
    33721 acgtaccgtc agcaacctgt aacagcagat taccgagaat cggtagcgta ggacgaccac
    33781 ctaacggacc gctcacctgt tgtagcggtt ttaataaatg ctcacgttct acggtaaatt
    33841 tcataggttt acgatgacaa tgttctgatt aaattagaaa aatcttcttt gatatcgtgg
    33901 ctctcttcac gcaactgctc gatcttccgg caggcatgaa gcaccgtcgt gtggtcacga
    33961 ccaccaaagg catcgccaat ctccggaaga ctgtggttag tcagctcttt cgccagcgcc
    34021 atcgccatct ggcgcggacg ggccaccgag cgggatcgac gcttggaaag gagatccgcg
    34081 actttgatct tgtagtactc cgccaccgtc ttctgaatat tgtcgatggt gaccagtttt
    34141 tcctgcaatg ccagcaagtc acgcagcgcc tcacgcacga agtcgatggt gatcgcccgt
    34201 ccggtaaagt tggcgttggc aatgacgcgg ttcagcgcgc cttccaactc acgtacgtta
    34261 gatcgtagac gcttggcgat aaagaacgcc acttcgcccg gcaaacgaat gtcgttttcg
    34321 tcggcctttt tcatcaggat cgccacgcgg gtttccagct ctggcggttc gatcgccaca
    34381 gtcagtcccc agccgaagcg ggatttcaaa cgatcctcaa cgccgttgat ctctttcgga
    34441 tagcgatcgg aggtgagaat gatctgttga ttaccttcca gcagggcgtt gaaggtgtgg
    34501 aaaaactctt cctgagatcg ttctttatta gcaaaaaact gaatatcgtc gatcagcagt
    34561 gcatctacgg aacggtagta gcgtttaaac tcttcgatcg cgttgttttg cagggcttta
    34621 accatgtcct gaacaaagcg ctcggagtgc atataaacca ctttggcatt cggcttgcgc
    34681 gccataatgc cgttacccac cgcatgcagc aggtgagttt tacccaaacc cgtgccgcca
    34741 taaaggaaca acgggttata agcaccgcca gggttatctg ccacctggcg agccgccgcg
    34801 cgcgccagtt ggttagattt accttcaacg aagttatcaa acgtgtgttt gacgtttacg
    34861 ttagaacgat aggtcggttc tgccggagcc gggacgttat cccaacccga gcgcgtagaa
    34921 ggcgcagcac gttgcggctg cgtttgcgcc acctgtgcag gggccgcgac gttgctcgtc
    34981 actgccgctt gtggcgtttg cgtcaccggt ttggtgccga cttcaaaacg cagctgtggg
    35041 gcatccgctc cgcagaaact ggttagcagt ccattgatat tattaaggta cttgtcccgt
    35101 acccaatcga ggacaaaacg gtttggcgcg tacagggcca gcgtgttatc gctcagttcc
    35161 gcctgcaatg ggcgtatcca catactgaat tctgtggctg gtaactcatc ctgcaatcgg
    35221 gcaagacact gctgccaaag cgaaagtgac acggcggact ccactcgaac aaaagtcgat
    35281 aatgactaag gctgaaacat tcatgattgt tgacgtacgt cgaaaagacc ctgcatgagg
    35341 gtgacgcacg aaccgctgtc tgcggttata tgcccgatca agatcctgca aaacgatcgg
    35401 gaccgcggat catagcctaa actgcgcaag agatcttctg tttctcacag attttcccga
    35461 tttatccaca ggatgatcca gaactcgcta agtgtaaacg atcctgccgc gggccgggca
    35521 cgatttacgc cgcatattgg aaaatttaat gaccatagac aaaaattggc ttaatcgatc
    35581 taataaagat ccaggacgat ccttgcgctt tacccgtcag cccgtataat cctccacccg
    35641 gcgcgccatg ctggttttca ctggtgtgag gccgtacgtt ttccccgcga aaaggtgcgg
    35701 aaaagcgcgg taaataagga aagagaattg actccggagt gtacaattat tacaatccgg
    35761 cctctttaat cacccatggc ttcggtgtcc atcgtttcat ttttcggcgg atatccaata
    35821 aagccattga atttattcaa gtttaggtag aaatcgccat gaaacgcact tttcaaccgt
    35881 ctgtactgaa gcgcaaccgt tctcacggct tccgtgctcg tatggctact aaaaatggtc
    35941 gtcaggttct ggcacgtcgt cgtgctaaag gccgcgctcg tctgaccgtt tctaagtaat
    36001 aaagctaacc cctgagtggt taagctcgca tttcccaggg agttacgctt gttaactccc
    36061 agtcaattca cattcgtctt ccagcagcca caacgggctg gcacgccgca aattaccatt
    36121 ctcggccgcc tgaattcgct ggggcatccc cgtatcggtc ttacagtcgc caagaaaaac
    36181 gttcgacgcg cccatgaacg caatcggatt aaacgtctga cgcgtgaaag cttccgtctg
    36241 cgccaacatg aactcccggc tatggatttc gtggtggtgg cgaaaaaagg ggttgccgac
    36301 ctcgataacc gtgctctctc ggaagcgttg gaaaaattat ggcgccgcca ctgtcgcctg
    36361 gctcgcgggt cctgatagcc ctcattcggg tctatcaacg cctgattagt ccgctactcg
    36421 ggccgcattg tcgtttcact ccaacctgtt caagctacgg aattgaggca ttgcgcaggt
    36481 ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt attaaaatgc caccctttac
    36541 accctggtgg tgacgatccc gtcccgcccg gaccatttga taccagagaa cactaacgat
    36601 ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc gtgtctttca tgatctggca
    36661 agcctgggag caggataaaa acccgcaacc tcaggcccaa cagaccacgc agacaacgac
    36721 caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc agtggccagg ggaaactgat
    36781 ctcggttaag accgacgtgc ttgatctgac catcaacacc cgtggtggtg atgttgagca
    36841 agccctgctg cctgcttacc cgaaagagct gaactctacc cagccgttcc agctgctgga
    36901 aacttcaccg cagtttattt atcaggcaca gagcggtctg accggtcgtg atggcccgga
    36961 taacccggct aacggcccgc gtccgctgta taacgttgaa aaagacgctt atgtgctggc
    37021 tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc gacgcggcag gcaacacgtt
    37081 taccaaaacg tttgtcctga aacgtggtga ttacgctgtc aacgtcaact acaacgtgca
    37141 gaacgctggc gagaaaccgc tggaaatctc cacctttggt cagttgaagc aatccatcac
    37201 tctgccaccg catctcgata ccggaagcag caacttcgca ctgcacacct tccgtggtgc
    37261 ggcgtactcc acgcctgacg agaagtacga gaaatacaag ttcgatacca ttgccgataa
    37321 cgaaaacctg aacatctctt cgaaaggtgg ttgggtggca atgctgcaac agtatttcgc
    37381 gacggcgtgg atcccgcata acgacggtac caacaacttc tataccgcta atctgggtaa
    37441 cggcatcgcc gctatcggct ataaatctca gccggtactg gttcagcctg gtcagactgg
    37501 cgcgatgaac agcaccctgt gggttggccc ggaaattcag gacaaaatgg cagctgttgc
    37561 tccgcacctg gatctgaccg ttgattacgg ttggttgtgg ttcatctctc agccactgtt
    37621 caaactgctg aaatggatcc atagctttgt gggtaactgg ggcttctcca ttatcatcat
    37681 cacctttatc gttcgtggca tcatgtaccc gctgaccaaa gcgcagtaca cctccatggc
    37741 gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt gagcgtctgg gcgatgacaa
    37801 acagcgcatc agccaggaaa tgatggcgct gtacaaagct gagaaggtta acccgctggg
    37861 cggctgcttc ccgctgctga tccagatgcc aatcttcctg gcgttgtact acatgctgat
    37921 gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg atccacgacc tgtctgcaca
    37981 ggacccgtac tacatcctgc cgatcctgat gggcgtaacg atgttcttca ttcagaagat
    38041 gtcgccgacc actgtgaccg acccgatgca gcagaagatc atgaccttta tgccggtcat
    38101 cttcactgtg ttcttcctgt ggttcccgtc aggtctggtg ctgtactata tcgtcagcaa
    38161 cctggtaacc attattcagc agcagctgat ttaccgtggt ctggaaaaac gtggcctgca
    38221 tagccgcgag aagaaaaaat cctgattcgg tgagttttcg ctaaaataag ggcggtcagt
    38281 tgaccgcctt ttttcttttc gtagggcgga taagcaccgc acatccgcca cacaaagcaa
    38341 caggaacatc atgagcgata atgacactat cgtagcccag gccacgcctc cgggacgtgg
    38401 cggcgttggc atcctgcgca tctccggctt caaagcccgt gaagttgccg aaaccgtgct
    38461 gggtaaactg cctaagccgc gctacgccga ttatcttccg tttaaagacg ccgacggcag
    38521 cgtgctcgat caggggattg cgctatggtt ccctggcccg aactcgttca ccggcgaaga
    38581 tgtgctggaa ctgcaaggtc atggcggtcc ggtgatcctc gacctgctgt taaaacgcat
    38641 tctgaccatt cccggcctgc ggattgctcg cccgggtgag ttttccgaac gcgcgtttct
    38701 taacgataaa ctcgacttag cccaggccga ggcgattgcg gatcttatcg acgccagctc
    38761 ggaacaggcg gcccgttcgg cgctaaactc gctgcaaggc gcgttctccg cacgggtcaa
    38821 ccatctggtg gaagccctca cccacttgcg catttacgtc gaagcggcga ttgatttccc
    38881 cgatgaagag atcgatttcc tctccgacgg caaaattgaa gcccagctca ataacgtaat
    38941 tgccgatctc gacgcggtac gcgccgaagc tcgtcagggt agtttgttgc gcgaagggat
    39001 gaaagtggtg attgccggac gtcctaacgc cggtaaatcg agcctgctga acgcgctggc
    39061 gggccgtgaa gcagcaatcg taaccgatat cgccggaacc acgcgtgacg tgctgcgtga
    39121 gcatatccac attgacggaa tgccgttgca tatcatcgat accgccgggc tacgtgaagc
    39181 cagtgacgaa gttgaacgta ttggtatcga gcgcgcatgg caggaaattg aacaggcaga
    39241 ccgcgtgctg tttatggtcg atggcaccac gacagacgcc gttgatccgg cagagatctg
    39301 gccggaattc atcgcccgtc tgccagcgaa actgccgatc accgtggtgc gcaataaagc
    39361 cgatatcacc ggcgaaacgt tggggatgag cgaagtgaac ggtcatgcgt taattcgtct
    39421 ttcggcgcgg actggcgaag gcgtggatgt actgcgtaac catctcaaac agagcatggg
    39481 ctttgacacc aatatggaag gcggcttcct ggcgcgtcgt cgtcacctac aggcgctgga
    39541 acaggcggca gagcatttgc agcaaggtaa agcgcaactg ttaggtgcct gggcgggtga
    39601 actgctggcg gaagagttgc gcctggcgca gcagaactta agcgagatca ccggggaatt
    39661 tacttcagac gacctactgg ggcggatttt ctccagcttc tgtatcggta agtaaccgcg
    39721 cttgcgaagc cgcattctga ctgtcagatg cggcttcgct tca
//
LOCUS       DAZFRG010000014        38468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFRG010000014 DAZFRG010000000
VERSION     DAZFRG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.014"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            235..1890
                     /gene="aslA"
                     /locus_tag="ACHKUC_000541"
     CDS             235..1890
                     /gene="aslA"
                     /locus_tag="ACHKUC_000541"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO6616856.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYITQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPARSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1981..3216)
                     /gene="aslB"
                     /locus_tag="ACHKUC_000542"
     CDS             complement(1981..3216)
                     /gene="aslB"
                     /locus_tag="ACHKUC_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6616857.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALSLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3363..3439)
                     /locus_tag="ACHKUC_000543"
     tRNA            complement(3363..3439)
                     /locus_tag="ACHKUC_000543"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3403..3405),aa:Pro,seq:tgg)
     gene            complement(3482..3568)
                     /locus_tag="ACHKUC_000544"
     tRNA            complement(3482..3568)
                     /locus_tag="ACHKUC_000544"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3532..3534),aa:Leu,seq:cag)
     gene            complement(3589..3664)
                     /locus_tag="ACHKUC_000545"
     tRNA            complement(3589..3664)
                     /locus_tag="ACHKUC_000545"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3629..3631),aa:His,seq:gtg)
     gene            complement(3723..3799)
                     /locus_tag="ACHKUC_000546"
     tRNA            complement(3723..3799)
                     /locus_tag="ACHKUC_000546"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3763..3765),aa:Arg,seq:ccg)
     gene            complement(3902..5287)
                     /gene="yifK"
                     /locus_tag="ACHKUC_000547"
     CDS             complement(3902..5287)
                     /gene="yifK"
                     /locus_tag="ACHKUC_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6616858.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5478..6218)
                     /gene="wecG"
                     /locus_tag="ACHKUC_000548"
     CDS             complement(5478..6218)
                     /gene="wecG"
                     /locus_tag="ACHKUC_000548"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO6616859.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6221..7573)
                     /gene="wzyE"
                     /locus_tag="ACHKUC_000549"
     CDS             complement(6221..7573)
                     /gene="wzyE"
                     /locus_tag="ACHKUC_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO6616860.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7570..8649)
                     /gene="rffT"
                     /locus_tag="ACHKUC_000550"
                     /gene_synonym="wecF"
     CDS             complement(7570..8649)
                     /gene="rffT"
                     /locus_tag="ACHKUC_000550"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO6616861.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSGHAREFMVAGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8646..9896)
                     /gene="wzxE"
                     /locus_tag="ACHKUC_000551"
     CDS             complement(8646..9896)
                     /gene="wzxE"
                     /locus_tag="ACHKUC_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO6616862.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9898..11028)
                     /gene="rffA"
                     /locus_tag="ACHKUC_000552"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9898..11028)
                     /gene="rffA"
                     /locus_tag="ACHKUC_000552"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO6616863.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAEAGR
                     IELPSIPDDCVQNAHMFYIKLRDIDDRSALISFLKEAEIMAVFHYIPLHASPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11033..11707)
                     /gene="rffC"
                     /locus_tag="ACHKUC_000553"
                     /gene_synonym="wecD"
     CDS             complement(11033..11707)
                     /gene="rffC"
                     /locus_tag="ACHKUC_000553"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO6616864.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKITLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11685..12566)
                     /gene="rfbA"
                     /locus_tag="ACHKUC_000554"
     CDS             complement(11685..12566)
                     /gene="rfbA"
                     /locus_tag="ACHKUC_000554"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6616865.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12585..13652)
                     /gene="rffG"
                     /locus_tag="ACHKUC_000555"
     CDS             complement(12585..13652)
                     /gene="rffG"
                     /locus_tag="ACHKUC_000555"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6616866.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWCKQVQDGSYQGERLGLKG"
     gene            complement(13652..14914)
                     /gene="wecC"
                     /locus_tag="ACHKUC_000556"
     CDS             complement(13652..14914)
                     /gene="wecC"
                     /locus_tag="ACHKUC_000556"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO6616867.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRVSELKIACFGLAFKPNI
                     DDLRESPAMEIAELIARWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14911..16041)
                     /gene="wecB"
                     /locus_tag="ACHKUC_000557"
     CDS             complement(14911..16041)
                     /gene="wecB"
                     /locus_tag="ACHKUC_000557"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6616868.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16097..17143)
                     /gene="wzzE"
                     /locus_tag="ACHKUC_000558"
     CDS             complement(16097..17143)
                     /gene="wzzE"
                     /locus_tag="ACHKUC_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO6616869.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17155..18258)
                     /gene="wecA"
                     /locus_tag="ACHKUC_000559"
     CDS             complement(17155..18258)
                     /gene="wecA"
                     /locus_tag="ACHKUC_000559"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO6616870.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGAVDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILLFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18498..19757)
                     /gene="rho"
                     /locus_tag="ACHKUC_000560"
     CDS             complement(18498..19757)
                     /gene="rho"
                     /locus_tag="ACHKUC_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO6616871.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19842..19943)
                     /gene="rhoL"
                     /locus_tag="ACHKUC_000561"
     CDS             complement(19842..19943)
                     /gene="rhoL"
                     /locus_tag="ACHKUC_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO6616872.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20084..20413)
                     /gene="trxA"
                     /locus_tag="ACHKUC_000562"
     CDS             complement(20084..20413)
                     /gene="trxA"
                     /locus_tag="ACHKUC_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO6616873.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20544..21809
                     /gene="rhlB"
                     /locus_tag="ACHKUC_000563"
     CDS             20544..21809
                     /gene="rhlB"
                     /locus_tag="ACHKUC_000563"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO6616874.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21945..23429
                     /gene="gppA"
                     /locus_tag="ACHKUC_000564"
     CDS             21945..23429
                     /gene="gppA"
                     /locus_tag="ACHKUC_000564"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO6616875.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23476..25497)
                     /gene="rep"
                     /locus_tag="ACHKUC_000565"
     CDS             complement(23476..25497)
                     /gene="rep"
                     /locus_tag="ACHKUC_000565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO6616876.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMLKGQSHLANLKAMMAAKRGK"
     gene            25584..25865
                     /gene="ppiC"
                     /locus_tag="ACHKUC_000566"
     CDS             25584..25865
                     /gene="ppiC"
                     /locus_tag="ACHKUC_000566"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO6616877.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25911..27386)
                     /gene="ilvC"
                     /locus_tag="ACHKUC_000567"
     CDS             complement(25911..27386)
                     /gene="ilvC"
                     /locus_tag="ACHKUC_000567"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO6616878.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27536..28426
                     /gene="ilvY"
                     /locus_tag="ACHKUC_000568"
     CDS             27536..28426
                     /gene="ilvY"
                     /locus_tag="ACHKUC_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO6616879.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28423..29967)
                     /gene="ilvA"
                     /locus_tag="ACHKUC_000569"
     CDS             complement(28423..29967)
                     /gene="ilvA"
                     /locus_tag="ACHKUC_000569"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO6616880.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQLQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29970..31820)
                     /gene="ilvD"
                     /locus_tag="ACHKUC_000570"
     CDS             complement(29970..31820)
                     /gene="ilvD"
                     /locus_tag="ACHKUC_000570"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO6616881.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSAQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31885..32814)
                     /gene="ilvE"
                     /locus_tag="ACHKUC_000571"
     CDS             complement(31885..32814)
                     /gene="ilvE"
                     /locus_tag="ACHKUC_000571"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO6616882.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32834..33097)
                     /gene="ilvM"
                     /locus_tag="ACHKUC_000572"
     CDS             complement(32834..33097)
                     /gene="ilvM"
                     /locus_tag="ACHKUC_000572"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO6616883.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33094..34740)
                     /gene="ilvG"
                     /locus_tag="ACHKUC_000573"
     CDS             complement(33094..34740)
                     /gene="ilvG"
                     /locus_tag="ACHKUC_000573"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO6616884.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34743..34793)
                     /gene="ilvX"
                     /locus_tag="ACHKUC_000574"
     CDS             complement(34743..34793)
                     /gene="ilvX"
                     /locus_tag="ACHKUC_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO6616885.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34880..34978)
                     /gene="ilvL"
                     /locus_tag="ACHKUC_000575"
     CDS             complement(34880..34978)
                     /gene="ilvL"
                     /locus_tag="ACHKUC_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO6616886.1"
                     /translation="MTALLRVISLVVISMVVIIIPPCGAALGRGKA"
     gene            35331..36851
                     /gene="yifB"
                     /locus_tag="ACHKUC_000576"
     CDS             35331..36851
                     /gene="yifB"
                     /locus_tag="ACHKUC_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO6616887.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRHRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36876..37214)
                     /gene="maoP"
                     /locus_tag="ACHKUC_000577"
     CDS             complement(36876..37214)
                     /gene="maoP"
                     /locus_tag="ACHKUC_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO6616888.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37333..38172
                     /gene="hdfR"
                     /locus_tag="ACHKUC_000578"
     CDS             37333..38172
                     /gene="hdfR"
                     /locus_tag="ACHKUC_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO6616889.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38268..38343)
                     /locus_tag="ACHKUC_000579"
     tRNA            complement(38268..38343)
                     /locus_tag="ACHKUC_000579"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38308..38310),aa:Trp,seq:cca)
     gene            complement(38352..38428)
                     /locus_tag="ACHKUC_000580"
     tRNA            complement(38352..38428)
                     /locus_tag="ACHKUC_000580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38392..38394),aa:Asp,seq:gtc)
BASE COUNT         9432 a        10512 c         9669 g         8855 t
ORIGIN      
        1 aaactaaaaa cagcatcaat aatcaacgcg atataacatc accgccttac atatcacctg
       61 cgccagaggt aggattgaaa atgctctcct gatttttcaa ttcattttct ggataaataa
      121 ataatttatt tttatcacta ttatttatgc aatcatcctg tcagggagag ggatctcaat
      181 tatcaatgct taattacgtc atcattttga cgacatgaaa ccggagcatt ttgcatggaa
      241 ttttcgtttt cacccaaact tcttgttgtt gctgtcgccg cagctcttcc tctcatagcc
      301 agcgcagcag ataccccgtc aaccgccacc gcacgcaaag gctttgccgg atacgatcac
      361 ccaaaccagt atctggttaa accggcgacc actattgccg acaacatgat gccggtgatg
      421 cagcatcctg cacaagacaa agaaacccag cagaaactgg cggaacttga gaaaaaaacc
      481 ggtaagaaac cgaacgtggt tgttttcttg ctggatgatg tgggctggat ggacgttggc
      541 tttaacggcg gcggcgtggc ggtgggtaac cctacaccag atatcgacgc cgttgccagc
      601 caggggctga ttttaacttc ggcgtattct caaccgagtt cttccccaac ccgcgccacg
      661 attctcaccg gacaatactc catccaccac ggcattctga tgccgccaat gtacgggcaa
      721 ccgggcggtc tgcaagggtt aaccacgctg ccgcagttgc tgcacgatca gggctacatc
      781 acccaggcta tcgggaaatg gcatatgggg gaaaacaaag agtcgcagcc gcagaacgtt
      841 ggctttgatg atttccgcgg ctttaactcg gtgtccgata tgtataccga atggcgtgat
      901 gttcatgtca acccggaagt cgccctgagc ccggctcgtt ctgaatacat ccaaaaactg
      961 ccgttcagca aagatgacgt tcatgccgtg cgcggtggtg aacaggaagc cattgccgac
     1021 attacgccga aatatatgga agacctggat caacgctgga tggaatatgg cgttaagttc
     1081 ctcgacaaga tggcgaagag tgataagcct ttcttcctct attacggcac tcgcggctgt
     1141 cacttcgata actacccgaa cgccaaatat gcgggtagct ctccggcgcg cacctcttac
     1201 ggcgactgca tggtggagat gaacgatatc ttcgccaatc tgtataaagc actggagaaa
     1261 aacggtcagc ttgataacac gctgattgtg ttcacctctg ataacggtcc ggaagccgaa
     1321 gttccgccgc acggtcgcac gccgttccgc ggtgcgaaag gttctacatg ggaaggcggc
     1381 gttcgtgtgc caaccttcgt ttactggaaa ggcatgatcc aaccgcgtaa atctgacggc
     1441 attgtcgatc tggcggatct cttccctacg gcgctggatc tggcggggca tcctggggcg
     1501 aaagtagcga atttagtacc gaaaaccacc tttatcgatg gtgtggacca gacatctttc
     1561 ttcctgggaa caaatggtca gtctaaccgt aaggccgagc attacttcct caacggtaaa
     1621 ctttctgctg tgcgtatgga tgagttcaag tatcacgtcc tgattcagca accttacgct
     1681 tatacccaga gcggatatca gggtggattc accggcacag taatgcaaac ggcgggatcg
     1741 tcggtgttta acctgtacac cgatccgcag gaaagcgact ctatcggcgt gcgccatatc
     1801 ccaatgggtg taccgctaca gaccgaaatg catgcgtata tggagatcct gaaaaaatat
     1861 ccaccacgcg cgcagattaa atctgattaa gccggcgcta tcgctgagat ctacctttgc
     1921 cggatgcgat gctgacgcat ccgacaatca accgcaggcg gccacaaaat ggctactttt
     1981 ttacttattc accaccagca aatgcgcctg cataatgtcg ctggccggac gaccgtgcgc
     2041 cagcaaatca accattgctt taagatatgg cggtagatgg cggaaataac gctgataccc
     2101 ggcacacaaa taattcagcc cgggtttgcc gctggcatcg agcataaagc ggtgtttcgg
     2161 gcagcctccc cagcacgctt ttaacacgtt gcaactgcga cactgtgccg gtaactgctt
     2221 gaatttatct tcaccaaatg cctgctgttg cggggaatca atcatttctg caatcatctg
     2281 ctgaagcata ttccctagtc gatactgcgg ataaacatag tgatcgcagg cataaacgtc
     2341 accgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc aaaccgcgcc
     2401 cggtgcgccg acaaaattgg cgaacgccca ttcgatattc attacgaaaa tcttgccgac
     2461 gtcgcgctta atccagtggt cgaaaatcgc caccagaaac tcaccgaact cttgcgggca
     2521 caccgaccat tccgtcagtt cgccctgaat atcgccggga gcatgtagct taagtccagc
     2581 atgagcagcc gtttcatcag ccaggcgctc gaccaccgga ataaactgga tgaactcaac
     2641 acctgcatca cacagaaaat catagacttg caaaggttgt aacgcactgg tgcgattgac
     2701 gcagaccagc acgttatagt cgacatgatg tttttgcagg agcgacaggg cacgcatcac
     2761 cagcttatgc gttggtctgc cacctttggt cacgcgatat tgattgtgga tctcagccgg
     2821 accatccagc gataacccta caagaaaatg attttctgcc agaaatgcac accattcgtc
     2881 atcgagcagc acgccgttag tctggaagct attacttatc ttcctgccag caccatattt
     2941 cgcctgtagc gccacggcac ggcggtaaaa atccagcccg agtaacgttg gttcgccgcc
     3001 ctgccaggta aaagccactt cgttttgggt ttcgctggca gcgatatagt gacggacata
     3061 cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt tcgtggtaaa gggattgttt
     3121 ttcgagataa aaacagtagt cacagttcag attacaatca gaaccgctcg gtttcgccat
     3181 cacatgaaaa gcacgcgttg gaacctgttg ctgcatggtc gctccttgcc tgaagtcatc
     3241 cttcaataag ttaagggcga aatcataccg tggcgcaatg cgttattacg ggcgtttatt
     3301 gcgaagggaa agtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc ggggttcaaa
     3361 attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca gttgcgctac
     3421 caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt ctttaaagtc
     3481 gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta
     3541 gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg gggtggctaa
     3601 tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac tgagctatag
     3661 ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc gccgggacta
     3721 aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc agcgctctat
     3781 ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga cttcccaccc
     3841 ctctgtctag tcccttttta aataaattgc gcgtttggtt acggtttgtg cgttttgctg
     3901 cttattcctc cagtttatgc gctttaccgt ggcgattaag cccaaaaact ttataaatcg
     3961 ccgtcaccac cagcataaag atgatgccga caaacagcga catacgcgta tcttcattaa
     4021 agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg gcccaaggga
     4081 acaggattga gcggaacgga tggctggcaa ttgccgcttt gtgtgcacgg cgaaaacgca
     4141 gctggcttat caaaatcaca aaccatggca ccatacccgg aagcacgctg gcgctgtaga
     4201 cgtagacaaa cacacgctgt ggattgggaa tgatgtagtt caggcatgaa ccaattagca
     4261 gaatagcaat agatactgcc acacccgcaa ccggtacgcc gtgacgggaa actttcgcca
     4321 ttgccgccgg taactgacgg tttttcgcca gtgcatagag catacgtccg caactgtaca
     4381 tgccgctgtt acagccagag agcgcagccg tcagcaccac aaagttgata atgcccgccg
     4441 ctgcggtaat gccgattttg gcgaaagtca gtacgaacgg gctaccgttg ctgcctattt
     4501 cattccacgg gaagatagta acgataacga aaatcgcgcc aacgtagaaa atcaggattc
     4561 gccacagcac cttgcctacg gcactgcgca gcgtcacctg cggattcttc gcttcaccgg
     4621 cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac aatgcggtca
     4681 ggaacccttt ccagccacca gcaaagaaac cgccatgctc tgtgagattg ctaaaaccaa
     4741 tcgactgccc gccattgcca aagccaaaga aaatcacgcc caggccaatg acaatcatca
     4801 cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg tacaacctca
     4861 ccgcagccag attcgccaac gccaccagcg ccaccgcgat caatgcgggt atccactgcg
     4921 ccatctccgg gaaccagaac tggacataaa cgccaatggc ggtgatttct gagatcccca
     4981 ccgccatcca cataaaccag taagaccagg cggtgagata accaaagaac gggctcatat
     5041 aacgatgcgc gtaaacggcg aacgaaccgg taaccggttc gaggaacaac atttcgccca
     5101 ttgaacgcat gatgaaaaag acgaacagcc cggcgatgat ataggccaac aatacggatg
     5161 gcccggccca tttcagggta ctggcggccc ccataaacag gccgacgcca atggtgcccc
     5221 ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct ggtttgttat
     5281 ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt atcgttatgg
     5341 gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata aagcattaaa
     5401 aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac gaagtgacca ctccgtcgct
     5461 ttacaaagag aggaaaatca gaggttgccg gtgtaatgcc agcgtaaata acgcagcaaa
     5521 cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca ctccagcccc
     5581 agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg tgaaaacatc gtaggtcccg
     5641 ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat gaagatctcc
     5701 tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg aatgcgttca
     5761 aacagcgcct gacgctgctc gggtttaaaa taaccatcct gactgccaac gatattcaca
     5821 ttccactggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt accgcccaca
     5881 agaaataccg gtgtcccttc tttgcctgcg cgcgccatca gctcttccca gagatcggcc
     5941 ccagcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg tacaacgctg
     6001 atgccatccg catatttaaa ttcggcagcg ttaattaact ccctgacctc ggcgttatct
     6061 tcaatggtca gcattttttc ggcattaatg gcaaccagcg ttccctgctt aagctgcccg
     6121 tcagcgaaca gataatcgag ggcatgctgc atatcacgcc aaccaatcaa ctgtaagcca
     6181 cgcagcgtat aggtcggtgc cgtggtgttg ttattcattg ttatccttca acctgcgtcc
     6241 ggagcgatga ttttgttcgt ttatgaatga gtccggcgct ttcaaaaagc cagtacaaca
     6301 gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagaccacg cgtgagacaa
     6361 acgaatccag cccttcacgc gccagcacga tcatattgaa gatcgccccg aagcagaaac
     6421 tgtgcaatat cgcagcctta tagcgattag tctcgcggtt gcccagctca tacagccagt
     6481 cgaaccattt gataatcagt ccaaccacaa tcgccccgag cgggatgaac aacgcgccgc
     6541 ccataacaac cagtgagcct ataagcgtag gcgagatcgc cagtccggag tggttattca
     6601 gcacttccca ggtaaagtag ttggcggagt tcagcaccat actcgggcga cccggccaca
     6661 gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc tggaagtcga
     6721 tgttgtcgta gttctgcaac agcaacgcca gattctccca cggcgagaaa gtgtcgcgag
     6781 tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca tagcgtttta
     6841 gcgccagcca gaacatgccc acaatcccca gcacgcccgc cgccgccagc atccacaacg
     6901 aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg atgatattgg
     6961 cgcgagtgcc gccgacaatc atgtaagtca acaagccaaa ggcaaccgtg ctgacgagga
     7021 aaaacagcca cgctttgcta tcctggcgca gaaaataaac caccagcatc gccgggatga
     7081 aaaagtaaaa gaagcgtttt aatgccacgc cggagacttc gctggaaaag atctgactgt
     7141 aagaattaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg acgctcacca
     7201 gagcgatgcc catcaggatc acccacgtga gattggtctc cacgcggttc atggtaaaca
     7261 gcggacggcg cggtgcatca gcaacgcgtt tgcgtagacg ggttttgtag gtgacatagt
     7321 aaaccgcgta gaagcagcct gcagaaagca acgcctgcaa caagatttct ggcggcgcga
     7381 caccaacatc aaagcgaaat accagcacgc tggtcagcgg aaagccgaag aaaaaggtga
     7441 gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga aactcaaacc
     7501 aggtcagcgt ggcaataaac agcgtgcaga gcagccagac aacaaacagg ccactgaatt
     7561 gcagcagact catgcgacct ccccggcggc aatcgccaac gcccgctgcc agccttgtag
     7621 atagttaggg ctaaagaagg caatggtgtt tttatccacc gacgccaact ggcgctgcgc
     7681 ttcacgcaca atatcctcgt tgagatcatc ggtagtaaac agtactggca aatgctgttc
     7741 cgtcatatcc tgccagaacg gattttcccg gttaagcacg caaggaatgc ccgcctgaat
     7801 cagtaagcaa agcgtaccaa tgccctgctg gcgggcaaaa ataaagtaac cgagatcgca
     7861 ctggcgaagt agcgtcagat aggcgtcaaa ttccagtttt tcgctcagaa cttgtaggtt
     7921 ttcttcgctg aataactcca gtcccgcctg acgaacttcc tcgatgtacg cttcgttatt
     7981 aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct gatgaacggc
     8041 gcgcaaggca gcaatatgct cattgctgcg gtcaccggag ttccccacca gaatggtcat
     8101 ttttccttca cgttgccgat cgttcgccat cgtattaaga gaagggtcca tccgcgtcgg
     8161 gaagtacagc agttctcccc gcacctttgg atgcgttttg gcaaaaaagc tcaaatcacc
     8221 gcgggtggca aatacacagc cgactctctt ttgcgccagg cgacgtagcg ggtaaaaaag
     8281 cttatatctc aagccactgg aaagctcata caggtctgct ccccagatat gccagaaaaa
     8341 ctggctgggc ttaatgccac cactcagcag agccagccac agtttgggat tgaactgacc
     8401 gtggaaaaaa aatcgctgct gacggttagc ttttgctttc gcgatgaccg cttccgccag
     8461 cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac ttaagccgtc
     8521 gtccttgcca gcaaccataa actcgcgcgc gtgcccgctc gtcgcggcca gcgcgtcgtt
     8581 gaaaaaccgc agaacggttc ggttatggtg agggatatcc gatcccagta cgtgaatcag
     8641 tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga aaaatagacg
     8701 atataggttg ccatataggc ctgtgctgcg cccagtgcgc catgcgcagg gattagccag
     8761 tgggcaaata ccatcaataa agtgaactgg ctgatttccg ccagaatata aaaccgcagt
     8821 gacgctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa cacatcaccc
     8881 accaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa cagcagccag
     8941 atagcaaaat cacgcagcaa ccagacggta aaactcgccg ccgccactgc cggtaagacg
     9001 aatttcagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag ccgcgacaac
     9061 gtgggcagca aatatacgct gaacgatgcc gtaataaatt gcaggtaggc atcggaaata
     9121 ctgctcaccc cttgccagat ccccacttca tcccagctat actgcgccgc cagcagttta
     9181 cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa tcaacgccat gagcgtaaat
     9241 ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct taacgggatg
     9301 acaccacgtt tgatcaacat gataacagca ggaattacca ccagcgcggg aatcagcgcc
     9361 agacccagca acgccccttc ataaccgccc aaacgataac tgacgtagta cgcgagaacg
     9421 ccaatcaagc taccgacaat cagagataac gcattacctg cggcatcgcg aaagcctttc
     9481 atcaacgcca gtaacaggtt gccccaggca atccccattt gcaccagcgc cactaaacgc
     9541 accagcccct gatagtcggt attaccaaac aacccctggc tgattggcgc agctgccagc
     9601 acaaaaacca gcgccattag cgtagagaag ccaagtacca tcgctgatga agtgccgacc
     9661 acacggcgca gctgttgggg attatcatga tactgggcaa cgtatttggt tacgccgtta
     9721 aagatgcctg ccccggcaag cacgccgagc acggtaatca actggcggaa atttgccgcc
     9781 agcccaagcc ccgccggacc aaatgacact gccagcaact taccgaccag taacccggca
     9841 ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa cgacatatca
     9901 ggagaagtag ttcaacaagg tcgcaattac cgtacgctga ttgacgggcg acaggttgta
     9961 gaacaacggc aggcgcagca agcgctcgct ctctttggtg gtgtagcgat cttcgccgtg
    10021 gaactcacca aagcgttccc ccgcagggct tgcgtgcagc ggaatgtaat ggaacaccgc
    10081 catgatttcc gcttccttca ggaagctaat caacgcgctc cggtcatcaa tatcccgcag
    10141 cttgatgtag aacatatgcg cgttctgcac gcagtcatcc ggaatcgacg gcagctcgat
    10201 acgcccggct tccgccagag gtgctaacgc atcgtagtag ttttgccaca gcgccagacg
    10261 ttgctggttg atgcgttcgg ctgcttccag ttgcgcccac aggtacgcag cttgcagatc
    10321 ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct gaccacggaa
    10381 gaactggctg cggtttgtgc ctttttcacg gatgatctcg gctcgttcga tcaacgcttt
    10441 atcgttaatc agcgtcgcgc cgccttcacc acccgccgtg tagtttttgg tttcatggaa
    10501 gctaaagcag ccaatatgac caatggttcc cagcgcacgc cctttgtaag tggacatcac
    10561 gccctgagcg gcatcttcca ccacaaacag attatgcttt ttcgccaacg ccataatggt
    10621 gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg ttttgtcggt
    10681 gatcgccgct tcaatcaacg tttcgtcgat gttcatggtg tccgggcgaa catccacaaa
    10741 aacgattttt gcgccacgca gcacaaaggc attggcggtg gagacaaagg tgtagctcgg
    10801 catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct ccagcgaagc
    10861 ggtgcaggat ggcgtcagta acactttggc gctgccaaaa cgttgctcca gccactgctg
    10921 gcagcgacgg gtaaaaccgc catcgccgca cagtttgccg ctacccattg ccgactgcat
    10981 atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt gatcacctgt
    11041 ataaccagta cgcggtgctt tctacattcg caccactttg tatatatcgt ttaagcgcgg
    11101 cggtgttgcc catctgggtc gccacccgca aagttatttt accgcgagca tacgcccagt
    11161 ttagcgccgt ttgcataagc tcagcacctg caccgcgtcc agccagcagg ccaattcgcg
    11221 catctgtcgc attgagttcc cgtaaagaga cataaccgcg aatatcgccg gacgccgcac
    11281 gtaaaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc cactgtgcat
    11341 aaaagcgacc gctggcgtca ggcgcatacc acggcgcacg aaaacggctt tgcgcaaatg
    11401 cggcgctggc taactgacgt aatgcgggaa tatctgtctc ttgtgccact tctgcgccgc
    11461 tatcaccgac attgttcacg ggtagcgcca aatcaacttc accttctacc agggagaatc
    11521 ccagctgttg cagggcatcc agttcacccg tatttgatgc cgcaattttg gcctgcaccc
    11581 gtgaccacgg cgctaacgcg tctggcgtca ggagcggtgc ttcagacgta atgcgcacga
    11641 tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata ctggcgcgga
    11701 cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgccag cgagctggcg
    11761 gcgcgcttca cgccttcgtc atcaagccag ccgttacgcc aggcgatctc ttccaggcag
    11821 gcaatcttaa agccctggcg tttttctact gtctgcacaa aagtgctggc ctcaatcagg
    11881 ctgtcgtgag tgccggtatc cagccaggca aatccgcgcc cgagcagttc aacggtcagg
    11941 ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc acgctccgac
    12001 ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag cccggtcacc
    12061 gcccagtttg atttcggctg ttttggtttt tcttccagcg agatagcgcg gaaattgtcg
    12121 tcaaactcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa taccgtcgcc
    12181 ccttccgtgc gcgccgcaac atgacgcagc ttcggactga agccctgtcc gaagaagatg
    12241 ttatcgccca gcaccagaca agaaggttca ccgttgagga aggtttcacc gatgataaag
    12301 gcctgcgcca gaccgtccgg actgggctgt tcggcgtatt ccagctggat accgaactca
    12361 ctaccatcgc ccagcaggcg ctggaaataa cctttatctt ccggcgtagt gatgatgaga
    12421 atttcgcgga taccggccaa catcagcacc gacagcggat agtaaatcat tggcttatcg
    12481 taaatcggca acagttgctt cgatacgccg cgcgtaatcg gatgcaatcg ggtgccggaa
    12541 ccgcccgcca ggataatacc tttcatgttt gcctccgctg aaaattagcc tttcagacct
    12601 aaacgctcgc cctgatagct gccgtcctgc acctgcttac accagctttc attagccaga
    12661 taccactgca ccgttttacg cattccactt tcaaaggttt cctgcggcag ccagccaagt
    12721 tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg acggtcagcg
    12781 acaaaggtga tcaggtcacg atagtgcgcc acgccgtgcg gcttattagg agccagttct
    12841 tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt gtgaccacca
    12901 atattatagg tttcaccgac tttccctgtg gtcgccacgc aatacagcgc gcgggcgtga
    12961 tcttcaacat acagccagtc acggatttgc tgcccgttgc catataccgg cagcggttta
    13021 cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg gtaaggaccg
    13081 tagttattcg agcagttggt gataagcgtc ggcaaaccat aggtccgcag ccaggcgcgc
    13141 accagatggt cgctgctggc ttttgacgcg gaataagggc tgctcggcgc atacggcgtg
    13201 gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc ggtggagata
    13261 tgatgaaaac ggaacgctga ttttttatct tccgtcagcg cagtccagta agcccgcgcc
    13321 gcttcaagca gtgtataagt cccgacaatg ttggtttcaa taaacgctgc cgggccgtca
    13381 atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg ctgatactca
    13441 gtgaatacgc gcgccagttc tgcccgatcg cagatatcaa ctttctcaaa ggcaaagcgc
    13501 tcgctttgcg cgaccggtgc cagagacatc aggtttccgg cgtaggtcag cttatcgacc
    13561 accaccaccg cgtcgctcgt ttcgttgatg atataacgca ccagcgccga gccaataaac
    13621 ccggcaccac ctgttatcag aatctttctc atcagcgcca gactcctttg gcatcgacga
    13681 catactgctg atggacgttg tcgccattga taactttgaa ctgactatga tcgaccagca
    13741 tcaccagcac atctgccgtt gccagcgcct cgtcaagctg cgccagagta caaagcccgg
    13801 tcagtttctt cggcaactgg tggatgttag gctcaacaac cagcgtttcg ccgctgtgcc
    13861 accgggcgat cagttcagcg atttccattg ccggactttc gcgcaggtca tcaatattcg
    13921 gtttaaacgc cagaccaaag caggcgattt tcagttcact gacgcgttta tcggtagcag
    13981 ccagacaatc agccaccgcc gctttcacct gatcgataac ccagaacggt ttgtgatcgt
    14041 tcacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc acgataaacc
    14101 acggatcaac agcgatgcag tgaccgccca cgccagggcc tggctgaaga atattgacgc
    14161 gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga tcggcacaaa
    14221 tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt tcggtgagct
    14281 tacacatttc tgccgtccgc gagttagtga cgacacactc accctcgagg aaaattttgt
    14341 acagttcgct ggcacgggcc gaacaaaccg gcgtcatacc accaatcacg cgatcgtttt
    14401 taatcagctc gaccataacc tggcccggta atacgcgttc cgggcagtaa gcaatgttga
    14461 cgtccgccaa ctcgcccacc tgctgtggga aagtgagatc tggacgcatc tctgctaacc
    14521 attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc accaacgcgc
    14581 cttttttcaa cactggcgcg atggagcggg cagccgattc gacgtacgtc atatctggct
    14641 catgatcgcc tttaaacggc gtcggtacag caatcaacca ggcatccgct tcaactggcg
    14701 tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc gccaaatcag
    14761 gctcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg ttgatatcga
    14821 caccaatcac ctgtttttgc cttgaggcaa acgcagctgc tgtaggcaac ccgatgtaac
    14881 ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat tttttaacgc
    14941 ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc ggctcatagc
    15001 ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc gctgcttatc
    15061 cgtgccgacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag tggtatcgcg
    15121 catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc ctgagtcggt
    15181 caaaatcagc caggcgtggt tcatcagcca gacaaacggt aaatactcct ggggatcgat
    15241 cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga cgttcgggtt
    15301 gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcgatgt ctgccagcgc
    15361 atggcagatt tcttcaaagc cacgaccgaa gctctcacgc ctgtgaccgg tcaccagaat
    15421 catcttttta tcggggtcga taaacgggta atttgccgcc agttctgaac gcagcgtatc
    15481 gctgctcatc acctggtcac gcacccataa cagtgcatca atgactgtat taccagtaat
    15541 gaagattcgg ctatccgcaa cattttcacg cagcaagttt tgccgggaag tttcggttgg
    15601 agagaagtga tacatcgcca gatgcccggt caatgtacgg ttagcctctt ccggccacgg
    15661 cgaatagaga tcgcccgtgc gcagaccagc ctcaacgtga ccaacaggaa tacgctgata
    15721 aaacgccgcc aggctggttg ccagcgtcgt cgtagtatcg ccgtgaacca gcacgacatc
    15781 tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg ttatctctgt
    15841 aaggccctgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg aaaagagttt
    15901 cagcacctga tcgagcatct cccgatgctg cgcagtgacg caaactttag cctcaaaaaa
    15961 aggatctttt gccaacgcat gcaccaacgg tgccatcttg atggcttccg ggcgcgtacc
    16021 aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagcg cctttgcgct
    16081 caccgcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca gccccgatca
    16141 gtcccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta tcgcgtttta
    16201 ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc agggttggac
    16261 caacattcag ggtgtttaac atggcccgat tctgatcata gtcgagatca aaggccggac
    16321 cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc aggaacattt
    16381 ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata ttatgctgct
    16441 cagcaatttt cagcgcctgt tcaatactgt tcatccggcg atcgtagatg gctttcgcca
    16501 cctcttcctg acgcttcacc tgagctttca tctggatggt gcgtgccgcc catgcgcctt
    16561 tcagctcatc attcagatgg ctggctgcac gctggctggc aaaagcaaca tactgacgta
    16621 acaggttatt agcgtcaggc gcagtttcgg caatcagctt cacgctgtca ttgaccgcgc
    16681 gggtgaagtc accgggaata aacacgatgt tgttgatcat ttcatccagc aacgccgcat
    16741 ctgctttgct gttgcccacc atccgctgct tgtaatagtc ggtttgcagc cagaactctc
    16801 tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataggcttcg tccatgaccg
    16861 atggttggtc ggcagaagcc atgtttgaac ggacatccag gttacgcaaa aattgctgct
    16921 gtgagtaata tccccccagc atattcaccg ttggacgatc ggtaatcgcc gtcgagctcc
    16981 actcctgacg agcaaaaaaa gtatacgcca gcgcgattaa cgcaaacgcc agccccatgc
    17041 caataatcca cagcttccca gcccacaagg tacgaaacaa cccacgaata tccagttcat
    17101 tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc tcatttattt
    17161 ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc gcttaataaa
    17221 gcgagcaact ttccaggcac gcttaatgca atatccatag aggaagaatg ctagcaaaaa
    17281 gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca cgccaatgga
    17341 agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc tggcgggaag taaacccggc
    17401 acgcatgatc aaatggtgaa tatgctgacg gtcaggagag aatgggctca tgcctttacg
    17461 caggcgacgg tacataatcg ccaccatatc cattagcgga atggcgataa tccacaaagc
    17521 ggtaaccggg ctgatgggat gagttttgcc ctgggtcgtt tcgagcagga tccagatcac
    17581 ggtaaaacca atcagcgtac tgcccgcatc ccccataaag actttgtagc ggcgacccag
    17641 tataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa agcaccagat
    17701 tgcgagactg gtttgcccgt cgaacaacaa aatcataccg attgctgcaa acgagacgca
    17761 ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat taatggccgc
    17821 ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct cccaggagcc
    17881 aaagatataa cccaggctac tgagataaag tttgccgaac accatcataa caatgccaac
    17941 agcggcctgt atggtggcac ggatttttac gctgatatca aaacggtcat ccaccgcgcc
    18001 aatgaaaaca agcacaccag cacaagcgag atagagagat gcatgcggaa tatagtaatc
    18061 aacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa cgagaggtat
    18121 caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt tttttgccac
    18181 cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga gatcagtact
    18241 cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga agtataacca
    18301 cgaagacctt tattcagaag ggaaagtccg ttccgaaccc accagtcctg tgcaaaaatt
    18361 aaccacttga tatgcaaatt gcataacaca ccagcaaatt ccaggaattt tcctagcatc
    18421 gccagcgcgg cattaagatt acagatataa aagcaaaacg ccacgtaaac acgtggcgtt
    18481 tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc attggtcttg
    18541 gtcattgcca gtttattaat gaggaattcc attgcatcga tttcgcccat cgggtgaatg
    18601 attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag ctcttcttta
    18661 cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc gatcttacga
    18721 gagaggtgca gttccatatt gcctgtacct ttaaactctt cgtagataac ttcgtccatt
    18781 ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc ctcttccacg
    18841 ttacgcgccg caccgaagaa gcgtttcgga cgatgcaggg cgttggcgtc cacaccaccg
    18901 gtcaacactt tacctgacgc cggaacaacg gtgttgtaag cgcgcgccag acgagtgatg
    18961 gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttcgc cttctcgatc
    19021 accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga agcaacaact
    19081 tcacctttta ccagacgctg catctcggtt acttcttccg gacgttcgtc gatcagcaga
    19141 accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt ctgcagcagc
    19201 atggttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg accgataggt
    19261 gatgccagat ccagtacgcg agcggttaaa tcttccgtag aaccgttacc acgttccata
    19321 cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt gttgcgggcg
    19381 ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata gcgttcacct
    19441 tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt gaaacggcgg
    19501 atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct gtctgcggaa
    19561 cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa gatatcttcg
    19621 ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg catacgagcc
    19681 aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg cgtattcttt
    19741 aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct cgaacttaat
    19801 gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact catgtctttt
    19861 cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt gagtgacgag
    19921 ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa ttacaagatt
    19981 caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga ctcacgccgg
    20041 gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc aggttagcgt
    20101 cgaggaactc tttcaactga cctttagaca gtgcgcccac tttggttgcc gccacttcac
    20161 cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc gcagtgccag
    20221 ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg tcagcgattt
    20281 catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag aaatcaacga
    20341 ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc aggtgaataa
    20401 ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt cgttgttggt
    20461 gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg taaagcaata
    20521 gtaagctgat attctaccac actatgagca aaacacattt aacagaacag aagttttccg
    20581 acttcgccct gcatccgaag gttgtagaag cccttgaaaa aaaagggttt cataactgta
    20641 cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta gccgggcagg
    20701 cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat tatcttctct
    20761 ctcatcccgc gattgccgat cgcaaagtga atcaaccgcg tgccttaatt atggcaccga
    20821 cgcgtgaact tgccgtgcag atccacgccg acgcggaacc gctggcggaa gctactggcc
    20881 tgaagctggg tctggcttac ggtggtgatg gctacgacaa acagctgaaa gtgctggaaa
    20941 gcggcgttga cattctgatt ggcaccacgg gtcgtttaat tgactacgcc aagcagaacc
    21001 acattaacct cggtgccatt caggtggtgg tactggacga agccgatcgc atgtacgatc
    21061 tgggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca aaccagcgcc
    21121 tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg ttcgagcaga
    21181 tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac cgtattaaag
    21241 aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg ctgatcgaag
    21301 aagagtggcc agacagagcg attattttcg ccaacaccaa acaccgttgt gaagagatct
    21361 ggggccacct ggcggcagat ggtcatcgtg tcggtttatt gacaggcgat gtcgcgcaga
    21421 aaaaacgtct gcgtattctt gatgaattta cccgtgggga tctggatatt ctggttgcca
    21481 ccgacgttgc cgcgcgtggt ttgcacatcc ctgctgtgac acacgtcttt aactacgatt
    21541 tacccgatga ctgtgaagat tacgttcacc gtattggtcg aacaggtcgc gctggcgcaa
    21601 gtggtcattc catcagcctg gcatgtgaag agtatgcatt gaatttgcct gctattgaga
    21661 cctatattgg tcactcaatt ccggtaagta aatacaatcc ggacgcattg atgaccgatc
    21721 tgccaaaacc gctgcgcctc acgcgcccgc gcacaggtaa tggtccgcgt cgtactggcg
    21781 ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaaaatgt catactccaa atcattcaag
    21841 ctgtatcaag atggcaagag aatgaatccc gatgagttta ctgtcgtaag tgattcaggt
    21901 gaaagaatgc agccaacaca gagacagatt gaaggatgaa gagtatgggt tccacctcgt
    21961 cgctgtatgc agccattgat ctcggttcga atagttttca tatgctggtt gtgcgcgagg
    22021 tggctggaag catccagacg ctgacgcgaa taaaacgcaa agtgcgtctg gctgccggcc
    22081 tgaatagcga aaatgccctg tctaatgaag caatggagcg cggttggcaa tgtctgcgcc
    22141 tgtttgctga acgtctgcaa gatatcccac cctcgcaaat tcgcgttgtc gctacggcga
    22201 cattacgcct tgccgtcaat gcgggtgatt ttattgccaa agcgcaggaa atcctcggtt
    22261 gtccggtaca ggtgatcagc ggtgaagagg aagcgcgtct gatttatcag ggcgttgctc
    22321 acaccactgg cggtgccgat cagcgcctgg tagtggatat cggcggagcc agtactgaac
    22381 tggtaaccgg cacgggtgca caaaccacct ctttgttcag cctgtcgatg ggctgcgtta
    22441 cctggctgga acgctatttt gccgatcgta atctggggca ggaaaatttt gatgctgcag
    22501 aaaaagcggc acgcgaagtg ttacgtccgg ttgccgatga attacggtat cacggctgga
    22561 aagtgtgcgt tggcgcttcc ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg
    22621 ggatggatga acgcattacc ctggaaaagt tgcagcaact gaaacaacgc gccattcatt
    22681 gcggtcggct ggaagagcta gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc
    22741 cgagtggtct ggcgatcctg atagccattt ttaccgaact gaatattcag tgtatgaccc
    22801 tggcgggcgg tgcgctgcgt gaaggcctgg tctacggtat gttgcatctt accgtcgagc
    22861 aggatattcg cagccgtacg ctgcgtaata ttcagcgccg ctttatgatc gatattgatc
    22921 aggcacagcg cgtagccaaa gttgcggcta acttcttcga tcaggtggaa aatgaatggc
    22981 atcttgaagc aataagccgc gatttgctca tcagcgcctg tcagcttcat gaaatcggcc
    23041 tgagcgttga cttcaaacaa gcgccgcaac acgctgctta tctggtacgt aatctggatc
    23101 ttcccggttt tacccccgca cagaaaaaac tgctggcgac gctactgctc aaccagacta
    23161 atccggtcga tctctcatcg ctgcatcagc aaaatgccgt accaccgcgc gtcgcagaac
    23221 aactctgccg tttactccgc ctggctatca tttttgccag ccgtcgccgt gacgatctcg
    23281 tgccagagat gacattacag gctaaccatg aactgttgac cttgacgctt ccgcaaggtt
    23341 ggctaaccca acatccgctg ggtaaagaga ttattgatca ggaaagccag tggcagagct
    23401 atgtccactg gccgctggaa gtgcattaat gagtaagtgc cggatgcgat gctgacacat
    23461 cttttccggc cttgattatt tccctcgttt tgctgccatc atcgctttca gatttgccaa
    23521 atgactttgc cctttcagca tccgttcttc ggcgctgacc actttgcgct cctgttccca
    23581 aatcaaatca tcctgcggca gctccagcag gaagcggctc ggctccgggc gcaccagttc
    23641 gccgtactga cggcgttctt tacacagcgt aaaggtcaat tccttctggg cgcgggtaat
    23701 gccgacatag gccagccgcc gctcctcatc gatattatct tcatcgatac tgctctggtg
    23761 cggcagaaat ccttcttcca taccgaccat gtaaacataa ggaaactcca gtccttttga
    23821 cgcgtgcagg gtcatcagtt gcacctgatc cagctcttcc tcgctctcgc cgcgctccat
    23881 catgtcgcgc agagtaaagc gcgtcaccac ctgggtgagc gtcatcggct catccagttc
    23941 gctgccttcc agcatctccg tcatccagct aaacagttgg ttgacgttct tcatgcgcat
    24001 ttcggcagct ttcgggctgg gcgatgtttc gtacagccag gattcataat ccatgccgtg
    24061 gatcagatcg cgcaccgcgg caatcggctc ccgctccgcc agacgctgga tttctgccag
    24121 ccagtgagtg aagcgggtca atgcttcata accacgtccg ctaagcgtct gactcaggcc
    24181 catatcaaag ctggcggtaa acatgctttt attgcgcgtc atcgcccatt cacctagctt
    24241 tttcagcgtc gccgggccaa tctctcgctt cggcgtgtta acgatacgca gaaatgcgct
    24301 gtcatcgtcc gggttagtca gcacgcgcag ataagccagc aagtccttga tttcaggacg
    24361 agagaaaaac gacgtaccgc cagatatttt gtacgggatg cggttttgca tcaggaattt
    24421 ttcaaatacc cgcgactgat ggttgccgcg ataaagaatg gcgtaatctt tgtactgcgt
    24481 tttattgacg aagtgatggg cgatcagctc gccggtgacg cgctcggctt catgttcctc
    24541 gttattcgcg cttaaaactt ttagctctgt gccgtaaccc agttcggaga acagacgctt
    24601 ttcaaagacg tgcgggttat tggcgatcag gatgttcgcc gctttcaaaa tacgtccgga
    24661 agagcggtag ttctgctcaa gcttaatcac cttcagcgcc ggaaaatcct ggctcagcag
    24721 caccagattc tgcggacgtg cgccgcgcca ggagtagatc gactggtcgt catcgcccac
    24781 cacggtaaag cgcgcgcggc tgcccaccag cagtttcacc agttcatact ggctggtgtt
    24841 ggtgtcctga tactcatcca ccagcaggta gcgaattttg ttctgccagc gctcgcggac
    24901 ttcttcattg cgttgcagca gcaacgttgg caataaaatc agatcatcga agtcgagaac
    24961 gttacaggct ttcaggtgtg catcatacag cccataacaa tgggcaaaaa tacggtcccg
    25021 ctcgcccttt gcctctgccg ccgcctgcgc cggtgttttg agatcgttct tccagttgga
    25081 gatggtcgaa attagctgtt gcaggagaac tttgtcatct tcaatcagcc cctcggtcaa
    25141 ctctttaagc aaagcaagct gatcggtatc gtcaaacagc gaaaagttag ccttcatccc
    25201 cagcgccgca tactcgcgtt tgatgatatc cagccccagc gtgtggaagg tggaaatcat
    25261 cagcccacgc gcctctttgc gccccagcgt ctgcccgaca cgctctttca tctcgcgtgc
    25321 cgccttattg gtaaaggtca ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat
    25381 cagatgggcg attttattgg tgataacacg agttttaccg gaacccgcgc ccgccagcac
    25441 caggcagggg ccggtaacga attcgacagc ttgttgttgg ccggggttta gacgcatagg
    25501 aaaatcgctc aatgaaagtc gggaggggaa aataacagcg tggtagtata gccagcctca
    25561 tccttaccac tcaaggcacg atcatggcaa aaacagcagc agcactgcat atccttgtaa
    25621 aagaagagaa actggctctg gatcttctcg agcagatcaa gaacggtgcc gatttcggca
    25681 agctggcgaa gaaacactcc atttgcccgt caggcaaacg cggcggtgat ttgggtgaat
    25741 tccgccaggg tcaaatggtt ccggcgttcg ataaagtggt tttctcttgt ccggtactgg
    25801 agccaaccgg cccgctgcat acccagttcg gttaccacat cattaaggtg ctgtaccgta
    25861 actaacagca aggccttctc cgggagaagg ccttgaactc tgaatcgcta ttaacccgca
    25921 acagcaatac gtttcatatc cgtcatatag ccgcgcagtt tcttacctac ctgctcaatc
    25981 gcatggctgc gaatcgcttc atttacatca cgcagctgcg cgttatctac cgcaccttcc
    26041 ggaatagctt tacccaggtc gcccggttgc agctctgcca taaacggttt cagcagtggc
    26101 acacaagcgt aagagaacag atagttaccg tactcggcag tatcggagat aaccacgttc
    26161 atttcgtaca gacgcttacg ggcgatggtg ttggcaatca gcggcagttc gtgcagtgat
    26221 tcatagtaag cagattcttc gatgatgccg gaatcaacca tggtttcaaa cgccaactca
    26281 actcctgctt ttaccatcgc gatcatcagt acgcctttat cgaagtactc ctgttcgccg
    26341 attttgcctt catactgcgg cgcggtttcg aatgcggttt tgccagtctc ttcacgccag
    26401 gtcagcagtt tcttatcgtc gttggcccag tcagccatca tgccggagga gaattcgccg
    26461 gagatgatgt cgtccatatg tttctggaac agcggcgcca tgatctcttt caattgctca
    26521 gagagcgcgt aagcacgcag tttcgccggg ttagaaagac ggtccatcat cagggtgatg
    26581 ccgccctgtt tcagcgcttc ggtgatggtt tcccaaccga actgaatcag tttttctgcg
    26641 tatgccggat cggtgccttc ttccaccagc ttgtcgaagc acagcagaga accagcttgc
    26701 aacataccgc acaggatggt ttgctcgccc atcaggtcag atttcacttc cgcaacgaaa
    26761 gaggattcca gaacgcccgc acggtgaccg ccggttgcag ccgcccatgc tttagcgatc
    26821 gccatgcctt cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt cggtacgccg
    26881 aatccacgtt tgtactcttc acgtacttcg gtgccagggc atttcggcgc aaccattacg
    26941 acggtgatgt ctttacggat ctgctcaccc acttctacga tattgaaacc atgagagtag
    27001 cccagcgccg cgccgtcttt catcagcggc tgaacggaac gcactacgtc agagtgctgc
    27061 ttgtctggcg tcaggttaac caccagatcc gcctgcggga tcagttcttc gtaagtgcca
    27121 actttgaagc cgttttcggt cgctttacgc caggatgcgc gcttctcggc aatcgcttct
    27181 ttacgcagag cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcaga
    27241 ccctgtgcac cacagccgac gatgactact tttttaccct gaaggtagct cgcgccatcg
    27301 gcgaattcat cgcgccccat aaagcgacat ttgcccagct gtgccaactg ctggcgcaga
    27361 ttcagtgtat tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt
    27421 cggttcgcgt ttgcgagatt gaattcacta tatgacagga aatttattgc ggaaattgat
    27481 atattcacaa cgtcacattg caatttctgc aacgtcaaca ttaagggctg cgccattgga
    27541 tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc
    27601 gcgggcaatg cacgttagcc cgtccacgct ctctcggcag attcagcgcc tggaagacga
    27661 tctcggtcag ccgctgtttg tgcgcgataa ccgcacggta acgctaaccg aagcaggcga
    27721 agaactgcgc gttttcgccc agcaaacgct gttgcagtat caacagctgc gccacaccat
    27781 cgatcagcaa ggaccgtcgc tctctggcga attacatatc ttctgctcgg tgaccgctgc
    27841 ctacagccat ctgccgccga ttctcgatcg cttccgcgcg gaacacccgt cggtggagat
    27901 taaactcacg actggtgatg cggcagatgc gatggaaaag gtcgtcactg gcgaagcgga
    27961 tctggcgatt gccgggaaac cggagactct gcccggcgca gtggcatttt cgatgctgga
    28021 gaatctggca gtagtgctga ttgcccctgc gctaccctgc ccggtgcgta accaagtttc
    28081 ggcagataag ccggactggt ctacagtgcc gtttatcatg gccgatcagg gaccggtgcg
    28141 ccggcgcatt gaactgtggt ttcgacgcaa caaaatcagc aacccgatga tttacgctac
    28201 ggtcggcggg catgaagcga tggtgtcgat ggtagcgctc ggctgtggcg tagccttgtt
    28261 gccggaagtg gtgctggaaa acagcccgga accggtgcgt aaccgcgtga tgattttaga
    28321 gcgcagtgat gaaaaaacgc cgtttgaact gggcgtgtgt gtgcagaaaa agcggctaca
    28381 tgagccgctg attgaggcgt tctggaagat tttgccgaac cactaacccg ccaaaaagaa
    28441 cctgaacgcc gggttattgg tttcgtcgtg gcaatcgtag cccagttcat tcagccgggt
    28501 ttcaaaatcc ggttcatgat cgccaagctc gaacgccgcc agtacgcgcc cgtagtcggt
    28561 gccatggctg cgatagtgga acaaagaaat gttccagtgc gtacccagcg tgttgaggaa
    28621 gcgcaacagc gcgcccggcg attccgggaa ttcgaagctg tagaggcgtt cctgcaacgg
    28681 atgcgacgga cgcccgccga ccatatagcg cacatgcagc ttcgccattt cgtcgtcgga
    28741 gagatccact acgctgtagc cgccatcgtt gagcatctgc aaaatttctt tgcgctcttc
    28801 gagaccacgg ctcaggcgca caccaacaaa aatgcaggcg tttttggcat cggcaaaacg
    28861 gtagttgaac tcggtgaccg agcgcccgcc aagcagctgg cagaacttca ggaagctgcc
    28921 cttctcttcc gggatggtta ccgccagcaa cgcttcacgc tgttcgccca gttcgcaacg
    28981 ttccgagacg tagcgcaggc cgtgaaagtt cacgttagca ccagaaagaa tatgcgccag
    29041 ccgctcaccg cgaatgttgt gctgggcgat atattttttc attcccgcca gtgccagcgc
    29101 gccagagggt tccgccaccg cgcgcacatc ttcgaacagg tctttcatcg ccgcacatat
    29161 ggcatcgcta tcgacggtga tgatgtcgtc gagatactcc tggcataaac ggaaggtttc
    29221 gtcgccgatg cgtttcaccg caacaccttc ggcaaacagc cccacgcgtg gcagatcaac
    29281 cggatgacct gcatccagcg ccgctttcag gcaggcggag tcttccgctt ccacggcaat
    29341 cactttgatt tgcggcatca gttgtttgat cagtaccgcc acgcccgccg ccagaccgcc
    29401 gccgccgact ggcacaaata cgcggtcgag atgggcgtcc tgctggagca gttccagcgc
    29461 cagcgtgcct tgcccggcga tcaccatcgg atggtcgaac ggcggcaccc aggtgaagcc
    29521 ctgcagctgt gatagttcga tcgctttcgc ttttgcttca tcaaagttcg cgccgtgaag
    29581 aagcacttcg ccgccgaagc cgcgcaccgc gtcgactttg atatcggcgg tggcggttgg
    29641 catgacgatc agtgccttca cgcctaaccg tgcggaagaa aacgcgacgc cctgcgcgtg
    29701 gttacccgca gaagcggtga tcacgccgtg cgctttctgt tcttccgtca ggcccgccat
    29761 catggcatat gcgccgcgca gcttaaagct atgaactggc tgacggtctt cgcgtttcac
    29821 cagaatcacg ttatcgagac gcgacgagag tttttccatt ttttgtagcg gcgtaacctg
    29881 cgccgcttcg tagaccggcg cgcgcagcac cgctcttaaa tattcggcgc cttccggggc
    29941 gccggagaga ggttgtgagt ctgccatcat taacccccca gtttcgattt atcacgcacc
    30001 gcgcctttgt cggcgctggt tgccaggctg gcgtaggcac gcagcgcaaa ggaaacctga
    30061 cgttcacgat ttttcggtgt ccaggctttg ttgccacgag cttcctgcgc ttcacgacgc
    30121 gccgccagtt cggcatcgct tacctgtaac tgaatgccac ggttcggaat gtcgatagcg
    30181 atcaggtcac catcttcaat caggccaatg ctgccgccac ttgccgcttc cggtgagacg
    30241 tggccgatgg aaagaccaga ggtgccgcca gagaaacgac cgtcggtgat cagcgcacag
    30301 gctttaccga gacccattga tttcaggaaa ctggttgggt agagcatttc ctgcatcccc
    30361 ggaccgcctt tcgggccttc atagcgaatt actaccacat ctccggcgac aactttaccg
    30421 ccgagaatcg cttctaccgc gtcgtcctgg ctttcgtaga ctttcgccgg gccagtgaat
    30481 ttgaggatgc tgtcatcgac gcctgcggtt ttaacgatgc agccgttttc tgcgaaatta
    30541 ccgtagagca ccgccaggcc gccgtctttg ctgtaggcgt gttccagcga gcggatacag
    30601 ccattggagc gatcgtcgtc cagcgtatcc cagcggcaat cctgcgagaa tgcctgtgta
    30661 gtacgaatgc ccgccgggcc agcgcggaac atatttttta ccgcatcatc ctgggtcacg
    30721 atgatgtcgt attgttccag cgtttgcggc aacgtcaggc caagtacatt tttcacatca
    30781 cggttcagta accccgcacg atccagctcg ccgagaatac cgataacacc accagcacgg
    30841 tgaacatctt ccatatggta tttttgggtg ctcggcgcaa ctttacacag ctgcggtact
    30901 ttgcgggaga gcttatcgat atcactcatg gtgaagtcga tttccgcttc ctgcgccgcc
    30961 gccagtaggt gaagaacggt gttggtagag ccgcccatcg cgatatccag cgtcatggcg
    31021 ttttcaaacg ccgccttact ggcgatgtta cgcggcagtg cgctttcatc atcctgttcg
    31081 tagtaacgtt tggtcaattc aacaatgcgt ttaccagcat tgaggaacag ttgcttgcga
    31141 tcagcgtggg ttgccagcag cgagccgttg cccggctgcg acaggcccag cgcttcggtc
    31201 aggcagttca ttgagttagc agtaaacatc ccggagcagg aaccgcaggt tgggcaggcg
    31261 gatcgttcaa cctgagcgct ctgggagtca gaaactttcg ggtctgcgcc ctggatcatc
    31321 gcatcaacca gatcgagctt aatgatctga tcggacagtt tggttttccc ggcctccatc
    31381 gggccgccgg aaacaaagat caccggaata ttcaggcgca gggaagccat cagcatcccc
    31441 ggggtgattt tgtcgcagtt agagatgcag accatggcgt cggcgcagtg ggcgttgacc
    31501 atatattcaa cggaatcagc gatcagttcg cgagatggca gtgaataaag catgcccccg
    31561 tggcccatag cgatcccatc atccaccgca atggtgttga actctttggc aacgccgcca
    31621 gccgcttcaa tttgttcggc aaccagttta ccgagatcgc gtagatggac gtgacccggt
    31681 acaaattggg tgaacgagtt cacaaccgcg ataatcggct taccgaaatc ggcgtcggtc
    31741 attccggtgg cgcgccacag cgcacgagca cccgccatat tacgaccatg agtggtggtg
    31801 gcggaacggt acttaggcat actttattta ctcccagtgt ctgtctcgta aatgggacgg
    31861 tgcgtgccgt cccatttttt gtatttattg attaacttga tctaaccagc cccatttatc
    31921 ttcggtttcg ccagtgaaga ggccgaagaa ggcttgctga atgcgtttgg taaccgggcc
    31981 gcaacggcct tcaccaacct gaataccatc tacactgcgc actggcgtga tttctgccgc
    32041 cgtaccggac ataaagactt catccgccag gtacagggat tcgcgcgaca gcacctgttc
    32101 acgcacttca attcccagct ctttcgccag tttgatgatg gcatcacggg taatacccgg
    32161 cagcgcggag gaggtgaacg gtggagtgaa cagcacaccg tctttcactt caaacaggtt
    32221 ttctcctgcg ccttcagaga tataaccatt cacatccagc gcgatacctt cctgataacc
    32281 gtggcggcgc gcttcgctac ccaccaacag ggaagagagg tagttacctc cagctttcgc
    32341 tgcggttggg atggtgtttg gtgcagcacg gttccaggag gaaaccatcg catcgatccc
    32401 ctgctccagc gcttctgcgc ccagatacgc cccccacggg aaagcggcga taatcacatc
    32461 ggttgagtat cccgctggcg ggtttacgcc catgccaaca tcaccgacga aaatcagcgg
    32521 acggatatag gcgctggtga gattgttttt gcggatcacg tcgcgacaag cttccatcag
    32581 ctcatcaatg ctctgcgaaa ccgggaaacg atagattttg gcggagtcat gcagacgctg
    32641 catatgctca cgatggcgga atacaaccgg tcctttgtgc gagtcgtagc aacggatgcc
    32701 ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc acatgcacct tcgcgtcttc
    32761 ccagcgaacc atctccccat tgaaccaaat gtaatcagct ttcttcgtgg tcatttttat
    32821 attccttttg cgctcaggcg cggatttgtt gtgatgtggt tgtgctctgg cagatggcaa
    32881 cgtgtgcgac gtccaccagt ttatttaact gactaaacag taagtcgacc gaccgtgggc
    32941 tggcaacggt caattcgata tttatatttt gtgcatcgct ggcggcggcc atattcattg
    33001 agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac acgttctaag gtttccggat
    33061 tgaagcgagc cgatacattg acctgatgtt gcatcatgat aatttctcca acatttctga
    33121 attactggca cctggcggca ccagcggcca gacgttctca agttcgtcga ttgagacatg
    33181 aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt gccgcttcaa cctggtcttt
    33241 atgggtgatg tgttggccag ggatgccgaa ggcgctggct aacatgagga aatcggggtt
    33301 atcggtaagg gttgtttcgc tgtatcgttc ctggaaaaac agttgctgcc attgtcgaac
    33361 catccctaac cgttggttat cgagtaagac gattttcaac ggtaactgct tgcgttttac
    33421 ggtgcccagc tcttgcacat tcatcatgaa agagccgtca ccggagatac agacgacggt
    33481 atcgttcggt cgcgcgactt gtgcgccaac cgccgccggt aaaccaaaac ccatggtgcc
    33541 taagccgctg gaggtgatga aattttccgg gcgagtgtgg gcgatgtgct gcgcagccca
    33601 catctggtgc tgccccacat ctgtggtcac gacgcaatcc gcaggttttc gatccgacag
    33661 ttgttttaac aacaacggcg cgtagatagc gtcaccggga tggtcgtaac gccaggtatg
    33721 ttcatcacgc agctgcgcgc agtattgctg ccagtcattg atatttaacg gctgctgtaa
    33781 tgctggtaac agagcattta aatcaccctg taatgccaca tgtgcctgac gcagcttgtt
    33841 catttctgcc gggtcgatat ccatatggat aacgctggcg tgtggtgcga aggtgttcag
    33901 tttgccagtc acccggtcat caaaacgtgc acccacggcg atcagtagat cgcactcctg
    33961 cactgcgaag ttcgccgctt tggtgccgtg catccccagc atgcccagat agtacggata
    34021 atctgcttct actgcgccca gccctttcaa cgtacaggtg gcaggcattt ttgtggcagc
    34081 gagaaattca cgcaaagccg aaactgcctg cgccatgccc acgccaccgc caacgtacag
    34141 catcggtttt tgcgcttttg ccagcatctg acgcgcttgc tcgacttcgg catgtgggaa
    34201 agtcacttcg ttttcaacag tggtgaacca cggttccaga tcgccgctgg ctaactggat
    34261 atcttttgga atatcgacca aaaccggacc aggacgacca gagctggcga cgtcaaacgc
    34321 ttcggccatg atacgcggta actcttccag tgattgcacc aggaaactgt gcttggtgca
    34381 ggctaacgac aaccccagaa tatccacctc ctgaaatgcg tcagtgccga taaacggtgc
    34441 ggatacctga ccggtgatag caacaacggg gatggaatct aacagcgcgt ccgcaagccc
    34501 ggttatcagg ttggttgcac ctggaccaga cgtggcgatg caaacgccag ttttgccggt
    34561 agcacgggca taaccgatag ccgccattgc tgcaccttgc tcgtgtcggc acagcaagtg
    34621 ttccacgcca ccgtcataca atgcatcgta aaccggcata attgcgccac ccggataacc
    34681 gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc acccactgtg cgccattcat
    34741 agttagttcc ccgtcctgaa tcttgagaaa cagaattttg tgctgttatt cattgtctgc
    34801 tcctcagtta tgtttttaag gtcaaaaaaa acccccggac ctttcggtgc gggggtctta
    34861 gttcgttaag gcttgatctc taagcctttc ctcgtccaag tgcagccccg cacggtggga
    34921 taataatcac caccatgcta atcacgacca ggctaatcac tcgtagaagg gctgtcattt
    34981 tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag ttaccataga ttttgcgaat
    35041 agcacaagat attttttggc cgatttttat gatgtcttta ataatctcaa gagaaattgt
    35101 tgttttttaa aaagaaaata gtgagtgaaa aaaattcaat ttcagtatga atgttgttcc
    35161 cttcctcgta gttcatcctt tgcccgctgc atttttgcga gcatcattcc accgccagaa
    35221 caactcaact tcacttgcac aaataaacag gaatcagcca ctaaaattca cgaattagcg
    35281 ggctacagca gcgatatctt atgcacataa tggtgactca aggagggctt atgtcactgt
    35341 caattgttca tacccgcgca gccctgggag taaatgcgcc cccaatcact gttgaggtac
    35401 atatcagtaa aggtctacca ggcttaacga tggtgggctt accagaaaca acggtaaaag
    35461 aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat ccggcgaaaa
    35521 aaatcaccat caaccttgct ccagccgatc tgccaaaaga agggggacga tatgatttgc
    35581 ctatcgctat tgcgttactg gcggcctcag aacagcttac agccaataag ttagatgaat
    35641 atgaattagt cggagaactg gcgcttacag gcgctctgcg tggcgttccc ggcgcaatct
    35701 ccagtgcaac tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag
    35761 atgaagtggg gctaattaac ggtgaaggat gcctgatagc cgatcatctg caggctgtct
    35821 gcgcgtttct ggaaggtaag cacgctctcg aacacccgaa accaactaat gcagtatccc
    35881 gggcgctaca acatgatctc agtgatgttg tcggtcagga gcaaggaaag cgaggactgg
    35941 aaattaccgc cgctggcggg cacaaccttt tactgattgg accgccggga acaggtaaaa
    36001 caatgctcgc cagccgtatt aatggccttt tgccagattt aagcaatgaa gaggcactgg
    36061 agagcgctgc gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa tggcggcatc
    36121 gcccgttccg ctcacctcat cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa
    36181 ttccagggcc aggtgaaatt tcgctggcgc ataacggcgt gctttttctt gatgagctac
    36241 ctgaatttga acggcgtaca ctggatgcct tgcgagagcc gattgaatcc gggcagatcc
    36301 atctttcacg cacacgagca aaaataacct atccagcccg tttccagctt gtcgcggcaa
    36361 tgaatcccag ccctaccgga cattatcagg gaaaccataa ccgctgcaca ccagaacaga
    36421 cattacgtta tctcaaccgg ctctcggggc cctttctcga ccgcttcgat ctctcactgg
    36481 agatcccatt accgcccccc ggcattttga gtaaaacggt agtgccggga gaaaacagca
    36541 ccaccgttaa acaacgtgta atggccgcca gagagcgcca atttaagcgg cagaataaac
    36601 tgaacgcctg gctggataat ccggaaatac gccaattctg caagcttgaa agcgaagatg
    36661 cgcagtggct ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt
    36721 tattgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc
    36781 atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac
    36841 tactgacata aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct
    36901 tcagcacctt caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgcttg
    36961 atacgcgtca tatacttgga ccacacgcgt tctgcttctg tcactggttc acgttcgcca
    37021 cggcatactg ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc
    37081 aactcattga aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaagtca
    37141 ccatgacgag agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta
    37201 aagctttccg ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt
    37261 attagttacg cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt
    37321 gcggagagca ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt
    37381 cactttggtc gagcggctga atcgctctat ctgacccaat cagccgtgag ctttcgaatc
    37441 agacaactgg aaaatcaact gggtgtgaat cttttcaccc gccacagaaa caatatccgt
    37501 ttaaccgctg ccggagaaaa gcttcttcct tatgcagaaa cgctcatgag cacctggcag
    37561 gccgcccgca aggaggtggc gcatacctca cgacataatg agttttctat cggtgccagc
    37621 gcctcgctgt gggagtgtat gcttaatcag tggctgggac gtttgtatca aaatcaggat
    37681 gtccatacag gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag
    37741 ttacatgaac gccagcttga tcttcttatt acaactgaag cccccaaaat ggacgaattt
    37801 tgtagtcagt tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta
    37861 aagggagatc ttaactatct gcgactagag tgggggccag attttcaaca gcatgaggca
    37921 ggtttgatcg gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag
    37981 caacagattg cgatgcttaa tggttgcacc tggctacccg tcagttgggc acgtaaaaaa
    38041 ggcggcctgc ataccgttgt cgatagcaca acactttcgc ggccgcttta tgccatatgg
    38101 ctacaaaata gcgataaaaa tgcgttgatt cgcgatctat tgaaaattaa cgtgctggat
    38161 gaagtgtatt aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg
    38221 gttatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga
    38281 gagactcgaa ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc
    38341 cctaattagg gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
    38401 gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc ggtttataac
    38461 cggcttac
//
LOCUS       DAZFRG010000015        38192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFRG010000015 DAZFRG010000000
VERSION     DAZFRG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.015"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..519
                     /locus_tag="ACHKUC_000581"
     CDS             <1..519
                     /locus_tag="ACHKUC_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616890.1"
                     /translation="GLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAEIYSFGN
                     DTASANRVLHQHVPAFNTDHMIQYYINAKEWNNARELLIKEEMLGMHNLILLENICMQ
                     KTAQCMAHITFTLNKLTTQPAITKKDSVGNEQLYQIGKIYHKLGVKPEPKQQALIQSL
                     YDQASGSTSSTQ"
     gene            complement(579..1070)
                     /gene="yajQ"
                     /locus_tag="ACHKUC_000582"
     CDS             complement(579..1070)
                     /gene="yajQ"
                     /locus_tag="ACHKUC_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO6616891.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1238..2149
                     /gene="panE"
                     /locus_tag="ACHKUC_000583"
     CDS             1238..2149
                     /gene="panE"
                     /locus_tag="ACHKUC_000583"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO6616892.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTATWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2112..2702
                     /gene="yajL"
                     /locus_tag="ACHKUC_000584"
     CDS             2112..2702
                     /gene="yajL"
                     /locus_tag="ACHKUC_000584"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO6616893.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2756..4204)
                     /gene="thiI"
                     /locus_tag="ACHKUC_000585"
     CDS             complement(2756..4204)
                     /gene="thiI"
                     /locus_tag="ACHKUC_000585"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO6616894.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4410..4652
                     /gene="xseB"
                     /locus_tag="ACHKUC_000586"
     CDS             4410..4652
                     /gene="xseB"
                     /locus_tag="ACHKUC_000586"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO6616895.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4652..5551
                     /gene="ispA"
                     /locus_tag="ACHKUC_000587"
     CDS             4652..5551
                     /gene="ispA"
                     /locus_tag="ACHKUC_000587"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO6616896.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            5576..7438
                     /gene="dxs"
                     /locus_tag="ACHKUC_000588"
     CDS             5576..7438
                     /gene="dxs"
                     /locus_tag="ACHKUC_000588"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO6616897.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLQKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            7493..8467
                     /gene="yajO"
                     /locus_tag="ACHKUC_000589"
     CDS             7493..8467
                     /gene="yajO"
                     /locus_tag="ACHKUC_000589"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO6616898.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(8521..9039)
                     /gene="pgpA"
                     /locus_tag="ACHKUC_000590"
     CDS             complement(8521..9039)
                     /gene="pgpA"
                     /locus_tag="ACHKUC_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO6616899.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9017..9994)
                     /gene="thiL"
                     /locus_tag="ACHKUC_000591"
     CDS             complement(9017..9994)
                     /gene="thiL"
                     /locus_tag="ACHKUC_000591"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO6616900.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSNSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(10072..10491)
                     /gene="nusB"
                     /locus_tag="ACHKUC_000592"
     CDS             complement(10072..10491)
                     /gene="nusB"
                     /locus_tag="ACHKUC_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO6616901.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10511..10981)
                     /gene="ribE"
                     /locus_tag="ACHKUC_000593"
     CDS             complement(10511..10981)
                     /gene="ribE"
                     /locus_tag="ACHKUC_000593"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO6616902.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11070..12173)
                     /gene="ribD"
                     /locus_tag="ACHKUC_000594"
     CDS             complement(11070..12173)
                     /gene="ribD"
                     /locus_tag="ACHKUC_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO6616903.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12177..12626)
                     /gene="nrdR"
                     /locus_tag="ACHKUC_000595"
     CDS             complement(12177..12626)
                     /gene="nrdR"
                     /locus_tag="ACHKUC_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO6616904.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12777..13316
                     /gene="yajI"
                     /locus_tag="ACHKUC_000596"
     CDS             12777..13316
                     /gene="yajI"
                     /locus_tag="ACHKUC_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6616905.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQIKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            13615..14499
                     /gene="tsx"
                     /locus_tag="ACHKUC_000597"
     CDS             13615..14499
                     /gene="tsx"
                     /locus_tag="ACHKUC_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6616906.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14537..14884)
                     /gene="yajD"
                     /locus_tag="ACHKUC_000598"
     CDS             complement(14537..14884)
                     /gene="yajD"
                     /locus_tag="ACHKUC_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO6616907.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15014..15985)
                     /gene="secF"
                     /locus_tag="ACHKUC_000599"
     CDS             complement(15014..15985)
                     /gene="secF"
                     /locus_tag="ACHKUC_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO6616908.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(15996..17843)
                     /gene="secD"
                     /locus_tag="ACHKUC_000600"
     CDS             complement(15996..17843)
                     /gene="secD"
                     /locus_tag="ACHKUC_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO6616909.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(17871..18203)
                     /gene="yajC"
                     /locus_tag="ACHKUC_000601"
     CDS             complement(17871..18203)
                     /gene="yajC"
                     /locus_tag="ACHKUC_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO6616910.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18226..19353)
                     /gene="tgt"
                     /locus_tag="ACHKUC_000602"
     CDS             complement(18226..19353)
                     /gene="tgt"
                     /locus_tag="ACHKUC_000602"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO6616911.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19408..20478)
                     /gene="queA"
                     /locus_tag="ACHKUC_000603"
     CDS             complement(19408..20478)
                     /gene="queA"
                     /locus_tag="ACHKUC_000603"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO6616912.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20571..21152
                     /gene="acpH"
                     /locus_tag="ACHKUC_000604"
     CDS             20571..21152
                     /gene="acpH"
                     /locus_tag="ACHKUC_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO6616913.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSERWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(21157..22974)
                     /gene="malZ"
                     /locus_tag="ACHKUC_000605"
     CDS             complement(21157..22974)
                     /gene="malZ"
                     /locus_tag="ACHKUC_000605"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO6616914.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPVVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQEHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGVRNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23130..24503)
                     /gene="proY"
                     /locus_tag="ACHKUC_000606"
     CDS             complement(23130..24503)
                     /gene="proY"
                     /locus_tag="ACHKUC_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO6616915.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24579..25898)
                     /gene="brnQ"
                     /locus_tag="ACHKUC_000607"
     CDS             complement(24579..25898)
                     /gene="brnQ"
                     /locus_tag="ACHKUC_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO6616916.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKTSAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26305..27600)
                     /gene="phoR"
                     /locus_tag="ACHKUC_000608"
     CDS             complement(26305..27600)
                     /gene="phoR"
                     /locus_tag="ACHKUC_000608"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO6616917.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27658..28347)
                     /gene="phoB"
                     /locus_tag="ACHKUC_000609"
     CDS             complement(27658..28347)
                     /gene="phoB"
                     /locus_tag="ACHKUC_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO6616918.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28537..29739
                     /gene="sbcD"
                     /locus_tag="ACHKUC_000610"
     CDS             28537..29739
                     /gene="sbcD"
                     /locus_tag="ACHKUC_000610"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO6616919.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIITTGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            29736..32879
                     /gene="sbcC"
                     /locus_tag="ACHKUC_000611"
     CDS             29736..32879
                     /gene="sbcC"
                     /locus_tag="ACHKUC_000611"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO6616920.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     EKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDSLTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALASYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAEALALHQQHRPDGLDLSVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            33005..34189
                     /gene="araJ"
                     /locus_tag="ACHKUC_000612"
     CDS             33005..34189
                     /gene="araJ"
                     /locus_tag="ACHKUC_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO6616921.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(34458..35366)
                     /gene="mak"
                     /locus_tag="ACHKUC_000613"
     CDS             complement(34458..35366)
                     /gene="mak"
                     /locus_tag="ACHKUC_000613"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO6616922.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            35491..36402
                     /gene="rdgC"
                     /locus_tag="ACHKUC_000614"
     CDS             35491..36402
                     /gene="rdgC"
                     /locus_tag="ACHKUC_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO6616923.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36483..36563)
                     /locus_tag="ACHKUC_000615"
     CDS             complement(36483..36563)
                     /locus_tag="ACHKUC_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616924.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(36560..36898)
                     /locus_tag="ACHKUC_000616"
                     /pseudo
     CDS             complement(36560..36898)
                     /locus_tag="ACHKUC_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37046..37330)
                     /gene="ppnP"
                     /locus_tag="ACHKUC_000617"
     CDS             complement(37046..37330)
                     /gene="ppnP"
                     /locus_tag="ACHKUC_000617"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO6616925.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSKFHLQVAEPTSYLCRYL"
     gene            complement(37402..38079)
                     /gene="aroM"
                     /locus_tag="ACHKUC_000618"
     CDS             complement(37402..38079)
                     /gene="aroM"
                     /locus_tag="ACHKUC_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO6616926.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
BASE COUNT         9602 a        10271 c         9578 g         8741 t
ORIGIN      
        1 ggactaacca aatatgctca acatctcgca tctttcgctg aagaatttta tataaaagag
       61 caaaaatcac attctgaaag tgaaatgaga ggactatttt catcacttgc cgaaatatat
      121 agttttggta acgatactgc cagcgcaaac cgagtgttgc atcaacatgt ccctgccttc
      181 aatacagatc atatgattca atattatata aatgccaaag aatggaacaa tgccagggaa
      241 ttattgataa aagaggaaat gttgggtatg cataatctga tattgttgga gaacatttgt
      301 atgcagaaaa ctgcacaatg catggcacac attaccttta cgctcaacaa attgacaaca
      361 caacctgcaa taacaaaaaa agatagcgtg ggtaatgagc aactttatca gattggcaaa
      421 atctaccata agctgggagt taaaccagag ccaaagcagc aggctttaat tcagtcgctt
      481 tatgaccagg catcaggaag cacctcttca actcaataaa cacatggtca aaagggccac
      541 cacggtggcc ctgacaatca atagctatat ccagaaagtt aatcgcggaa gtttttgaac
      601 tggaacggct gacccagatc gccaccacgt accatcgcca tgacagcctg taaatcatca
      661 cgagatttac ccgttacgcg gatctcatcg ccctgaattt gcgcctgcac tttcagtttg
      721 ctgtctttga tcattttgac gattttcttc tgagtcgcgc tctcaatgcc ctgtttcagt
      781 ttcgcttcca caaaccaggt tttaccgcta tgaacgatat tttccggtac atccagtgaa
      841 ctgccttcaa tgccgcgctt cagcaacttg gcacgcagaa tatccagcaa ctgattgacc
      901 tggaagtcgg actcgctcaa cactttgatg gttttgctgg cgtcattcag ctcaaatgag
      961 gcttcaacgt tacggaagtc aaaacgggac tccacttcgc ggctcgcgtt atcgaccgcg
     1021 ttacgtgctt cctgaagatc aacttcagag acaatatcga aagatggcat cttttcctct
     1081 cccttcattt ttgatgcgaa gcataatacc tgcaaagtta aataaccaac agtcctgcac
     1141 caggacgtat cccttaattg tgcatagtcg caacagcatg cacaattaag ggatagcctt
     1201 attgaacacc cggagtggtt gcgggtgagg aggaacaatg aaaattaccg tattgggatg
     1261 cggtgcctta gggcaattat ggcttacagc actttgtaaa cagggtcacg acgttcaggg
     1321 ctggctgcgc gtaccgcaac cttattgtag tgtgaatctg gttgagacag atggttctat
     1381 atttaacgaa tcgctgaccg ccaacgatcc cgattttctc gccaccagcg atctgctcct
     1441 ggtgacgctg aaagcatggc aggtttccga tgccgtcaaa agcctcgcgt ccacactgcc
     1501 tgtaaccacg ccaatactgt taattcacaa cggcatgggc accatcgaag agttgcaaaa
     1561 cattcagcag ccattactga tgggcaccac cacccatgcc gcccgccgcg acggcaatgt
     1621 cattattcat gtggcaaacg gtatcacgca tattggtccg gcacggcaac aggacggcga
     1681 ttacagttat ctggcggata ttttgcaaac cgtattgcct gacgtcgcgt ggcataacaa
     1741 tattcgcgcc gagctgtggc gcaagctggc agtcaactgt gtgattaatc cactgaccgc
     1801 cacctggaat tgcccgaatg gtgaattacg tcatcatccg caagaaatta tgcagatatg
     1861 cgaagaagtc gcggcagtga tcgaacgcga agggcatcat acttcagcag aagatttgcg
     1921 tgattacgtg atgcaggtga ttgatgcaac agcggaaaat atctcgtcga tgttgcagga
     1981 tatccgcgcg ctgcgccaca ccgaaatcga ctatatcaat ggttttctct tacgccgcgc
     2041 ccgcgcgcat gggattgccg taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa
     2101 ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc ccctggtagt gaagagactg
     2161 aagccgtcac cactatcgat ctgctggttc gcggcggtat caaagtcacc actgccagcg
     2221 tcgccagcga tggtaacctg gcgattacct gctcgcgcgg cgtgaagctg ctggcggatg
     2281 cgccgctggt tgaagtggct gatggcgaat atgacgtgat tgtgctgcct ggtggcatca
     2341 aaggcgcaga gtgttttcgc gatagcaccc tgctggttga aaccgttaaa cagttccacc
     2401 gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc caccgtgctg gtcccgcacg
     2461 atatcttccc gattggcaat atgaccggct tcccgacgct gaaagacaaa atacccgccg
     2521 aacaatggca ggacaagcgc gtcgtctggg atgcacgggt aaaattgctg accagccagg
     2581 ggccaggtac agctatcgac tttggtctga aaattatcga cctgctggtt gggcgcgaga
     2641 aagcccatga agtggcatca caactggtga tggcggcagg gatttataat tattacgagt
     2701 agtgtcggat gcggtaaacg tcgcatctga ccagatgcga cgttacaacc acatattacg
     2761 ggcgatacac cttcacattg ttaaagccct gctcgcgcag atagagcgcc tgcaggcggc
     2821 tcatcacccc gcgctcacac cacaacagcc aggttttgct ctggtcgaga tcgccaaatt
     2881 tggtgctcag tttatagaac ggcagagaaa ccacatcaat cccttcgact ttcagtggct
     2941 tatcttcctg ttcatcgata gaacggatat cgaggatcac gtcgttcggg ccgaagccat
     3001 tgacggtttc cacttccacc acttcctgct cggtctgctg ggcgatttcg cggatatcaa
     3061 cgttattcgc ttcctccacc actttatcga gaatgctgaa gtcgaacttc tcttcttccg
     3121 cttcaatctt cgatttaacc gctttcaccg tcgggctttt ggaaatcacg ccgcaatatt
     3181 ccggcatcgt gcgggcaaag tcttcggtgc caatctggcg agccaggttg atgatgtgct
     3241 ctttgtcgta agagatcagc ggacgcagga tcagcgtatc ggagacgtta tcaatcaggc
     3301 gtaggttggt cagcgtctgg ctggacacct ggccgagcgc ttcaccggta accagcgcct
     3361 gtacgccgta acgttctgcc actttggacg cggcacgcac catcatacgt ttgaggataa
     3421 cgcccatctg accgtcgtcg attttctcga gaatttcccc gacgaccggt tcgaaattaa
     3481 tagcgacaaa acgcacgcgg tgggagctgc caaagcggtt ccacagataa tgcgccacct
     3541 gacgtacgcc aatttcatgc gccgcgccgc cgaggttaaa gaagcagtaa tgcacgcggc
     3601 agccgcgacg catcaacata taactggaaa caccggagtc aaaaccaccg gaaatgagcg
     3661 acaacacatc ttcctgggtg ccgatcggga aaccgccaat accttcgtag cggcctttaa
     3721 tcagcagcag acgatcgtct tccacttcca gatggacagt cacttccgga ttggtcagct
     3781 tcacgcgcgc ggattcaata tgctgattta aaccgccgcc gacgtaacgt tccacatcaa
     3841 tcgagctaaa atcatgtttg ccgcggcgct tcacgcgtac gcagaaggtt ttgccttcca
     3901 gctgatcgcg atattgaacc aacgctttct cgaaaatatc gtgcatgtct gtaaacggca
     3961 cgtcttcgac ttcgagaata tggtggatac ccggaatacg ggtcagggcg tcgcgaatag
     4021 ccagacgctg gttttcatct tttgcgcgaa cttcgatgtt atcccagtgg cggacgacag
     4081 cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg atttttataa
     4141 agcgcaagcg cacagattgg cttttgatgg tgatttccgg gaacaattta atgataaact
     4201 tcatggcggc aatggttcgt tggcaagcct taagcgactt gtatagggaa aaatacagca
     4261 gcccacacct gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc
     4321 acatcacctt accattgcgc gttatttgct atttgccctg agtccgttac catgacgggg
     4381 cgaaaaatat tgagagtcag acattcatta tgccgaagaa aaatgaggct cccgccagct
     4441 ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc
     4501 cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca gctggcacgt caggggcagg
     4561 ccaaattaca acaagccgaa cagcgcgtac aaattctgct gtctgacaat gaagacgcct
     4621 ctctaacccc ttttacaccg gacaatgagt aatggacttt ccgcagcaac tcgaagcctg
     4681 cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac
     4741 tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt
     4801 cctggtttat gccactggtc atatgttcgg cgttagcaca aacacactgg acgcacccgc
     4861 tgccgccgta gagtgtatcc acgcttactc attaattcat gatgatttac cggcgatgga
     4921 tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc
     4981 gattctcgct ggtgacgctt tacaaacgct ggcgttctcg attctaagcg atgccgatat
     5041 gccggaagtg tcagatcgcg acagaatttc gatgatttct gagctggcga gcgccagcgg
     5101 tattgccgga atgtgcggtg gtcaagcatt agatttagac gctgaaggca aacacgtacc
     5161 tctggacgcg cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt
     5221 tcgccttggt gcattaagcg ccggagataa agggcgtcgt gctctgccag tactcgacaa
     5281 gtacgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtagg
     5341 agatactgca acgttgggaa aacgccaggg tgccgatcag caacttggta aaagtaccta
     5401 ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc
     5461 ccgtcagtcg ctgaaacaac tgactgaaca gtcactcgat acctcggcac tggaagcgct
     5521 agcggactac atcatccagc gtaataaata aacaataagt attaataggc ccctgatgag
     5581 ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg agttacgact
     5641 gttgccgaaa gagagtttac agaaactctg cgacgaactg cgccgctatt tactcgacag
     5701 cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc
     5761 gctgcactat gtctacaata ccccgtttga ccagttaatc tgggatgtgg ggcatcaggc
     5821 ttatccgcat aaaattttga ccgggcgccg cgacaaaatc ggcaccatcc gtcagaaagg
     5881 tggcctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca
     5941 ttcatcaacc tccatcagtg ccggaattgg gatcgcagtt gctgccgaga aagaaggcaa
     6001 aaatcgccgc accgtctgtg tcattggcga tggcgcgatt actgctggca tggcgtttga
     6061 agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtggttctca acgacaatga
     6121 aatgtcgatt tccgaaaatg ttggcgcgct caacaaccat ctggcgcagc tgctttccgg
     6181 taagctttac tcttcactgc gcgaaggcgg aaaaaaagtt ttctctggcg tgccacccat
     6241 taaagagctg ctcaaacgta ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt
     6301 gtttgaagag ctgggcttta actacatcgg cccggtggac ggtcacgatg tgctggggct
     6361 tatcaccacg cttaagaaca tgcgcgacct gaaaggcccg cagttcctgc atatcatgac
     6421 caaaaaaggt cgtggttatg aaccggcaga aaaagaccca atcaccttcc acgccgtgcc
     6481 taaatttgat ccctccagcg gttgtctgcc gaaaagtagc ggcggtttac caagctattc
     6541 aaaaatcttt ggcgactggt tgtgcgaaac cgcagcgaaa gataacaagc tgatggcgat
     6601 tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat tcccggatcg
     6661 ctacttcgac gtggcaattg ccgagcaaca cgcagtgacc tttgctgcgg gtctggcgat
     6721 tggaggctac aaacccattg ttgcgatcta ctccaccttc ctgcaacgcg cctatgatca
     6781 ggtgctgcat gacgtggcga ttcaaaagct tccggtcctg ttcgccatcg accgtgcggg
     6841 cattgttggt gctgacggtc aaacccacca gggcgctttt gatctctctt acctgcgctg
     6901 cataccggaa atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta
     6961 taccggctat cactataacg atggcccgtc cgcggtgcgc tacccgcgcg gcaacgcagt
     7021 tggcgtggaa ctgacgccac tggaaaaact gccaattggc aaaggcattg tgaagcgtcg
     7081 tggtgagaaa ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc
     7141 tgaatcgctg aacgctacgc tggtcgatat gcgtttcgtg aaaccgcttg atgaagcgtt
     7201 aattctggaa atggccgcca gccatgaagc gctggttacc gtagaagaaa acgccattat
     7261 gggtggtgca ggcagcggcg tgaacgaagt gctgatggcc catcgtaaac cagtacccgt
     7321 gctgaacatt ggtctgccgg acttctttat tccacaagga actcaggaag aaatgcgcgc
     7381 cgaactcggc ctcgatgcca ccggtatgga agccaaaatc aaagcctggc tggcataatc
     7441 cctactctac tcctgctatg cttaagaaat tattcatggc aggagtggca gcatgcaata
     7501 caacccctta ggaaaaaccg accttcgtgt ttcccgactt tgcctcggct gtatgacctt
     7561 tggcgagcca gatcgcggta atcacgcatg gacactgccg gaagagagca gccgtcccat
     7621 cattaaacgt gcgctggaag gcggcataaa tttctttgac accgccaata gctattccga
     7681 tggcagcagc gaagagatcg tcggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt
     7741 ggtcgttgcg accaaagtgt tccatcgcgt tggtgattta ccggaaggat tatcccgtgc
     7801 acaaattttg cgctctatcg acgacagcct gctccgtctc ggcatggatt atgtcgatat
     7861 cctgcaaatt catcgctggg attacaacac gccgatcgaa gagacgctgg aagccctgaa
     7921 cgacgtggta aaagccggga aagcgcgtta tatcggcgcg tcatccatgc acgcttcgca
     7981 gtttgctcaa gcactggaac tacaaaaaca gcacggctgg gcgcagtttg tcagtatgca
     8041 ggatcactac aatctgattt atcgagaaga agagcgcgag atgctgccac tgtgttatca
     8101 ggagggcgtg gcggtgattc catggagccc gctggcgcgg ggccgtctga cgcgtccgtg
     8161 gggagaaact accgcacgac tggtgtctga tgaggtgggg aaaaatctct ataaagaaag
     8221 cgatgaaaat gacgcgcaga tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc
     8281 aacacgagca caagttgcgc tggcctggtt attgagtaaa ccgggcattg ccgcaccgat
     8341 tatcggaact tctcgggagg aacagcttga tgaactgcta aacgcggtgg atatcacttt
     8401 gaagccagaa cagattgccg aactggaaac gccgtataaa ccgcatcctg tcgtaggatt
     8461 taaataagag tccaggcctg atgagacgtg acaagcgtca catcaggcat cggtgcacaa
     8521 ctacgacaga atacccagtg gccagtgatg accgataaaa tacaggatgc cagcggagat
     8581 caccccggcg acaatatcat ctatcatgat tcccatgccg ccatgtacat tgcggtcaaa
     8641 ccagcggatc ggccacggtt tccacatatc gaggatacgg aaaatcacaa acccggctgc
     8701 gacccactgc cagtcattgg tcggcagcgc catgagcgtg atccacatac cgataaattc
     8761 gtcccagaca atgctgccat gatcgtgcac gcccatgtct ttcgccgttt gatgacaaag
     8821 atagacgccg atacagatcc ccagcattac caccagcgaa tagagctgcc agggcaaaaa
     8881 agtcatcaga taccagaagg gaatcgccgc cagcgacccc atcgtgccag gaacgatcgg
     8941 gcttaatcca cttccgaatc cgacagcaag cagatgccac ggattactca ttttcaggcg
     9001 acttttcgcg acatctttat ggcgtggcaa aatggtcata tcctttccag tctaacgtga
     9061 caggttcgcc gtcacgaata aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac
     9121 aggtaaacgg tacacccagg tgtccgagag ccacatccag cgcgccacgg ttcagttccg
     9181 gcaccgtgaa acacaactcg taatcttcac cgccagagag cgcccagcgc agcgcctgtt
     9241 ccggttcaac atggcgagaa agcgcatcag aaaacggcag caatgccagg tcaatacgtg
     9301 cgccgcagtc gctggctttc acgatatgcc cgagatcgga aatcagaccg tcagagagat
     9361 cgatagctga atttgccaga tcgcgcagtg cctgcccctg taaaatacgc ggcgacggac
     9421 ggagatgacg tttgatcaag tagtccgcat ctttagcatc ggcaacctgc aaacggtttt
     9481 gcaaaatcgc cagcccggca gcgctatcgc ccggtgtacc ggtcacatag atccagtcac
     9541 ccggtttcgc cccagagcgc gttaaggctc gtcccatcgg aacaaagccg tggataccca
     9601 acgtcattga taatggccca cgcgtggtat cgccgccaat gagttgcata tcgtaataat
     9661 tgagaagatc aaacaagctg ttgctgaagg actcaagcca cgcttcgtct acgtccggta
     9721 aggttaatgc caacgtcagc caggccggat cggcccccat cgctgccaga tcgcttaggt
     9781 tcaccgccag tgctttataa gccagatcag caggatcgat atcagggagg aagtggttgc
     9841 ccgccaccag cgtatcagtg ctgatcgcca gggtctgttt ctcggggata ttgagaagag
     9901 cgcaatcgtc gccgatgccc agttcgacat caagacgaga acttcttaca cggtcaaaat
     9961 aacgggcaat cagggagaac tcgccacatg ccatacgtta cgcctcagca gataaaaaag
    10021 aaaaggccgg cgatcatgga acggtcttcc gtgaatctac cggcctggat attacttttt
    10081 gttagggcga atcacaggtg ctgctttatc gagtacgccg ttgacgaact tatggctgtc
    10141 ttctgcgccg aaagatttcg ccagttcgat cgcttcgtta atggccactt tgtatggcac
    10201 atcgctacgt ttagacagct cgtacagcgc aatgcgcagt actgcttttt ctacctggcc
    10261 cagttcttcc agcagacggg acaggtacgg cttcatcagt ccgtcgaggt atgcggtgtt
    10321 agtcgccacc ccggccagca gctcacggaa gtacaggacg tcaacgtctt ttacatcctg
    10381 ttcagccagg aactggtatt caacatcagc gatgtcgttc tgggacaact gccaggagta
    10441 gagcgcctgg acggcacact cacgagcgcg gcgacgagca gcaggtttca cggatttccc
    10501 cttactaatt tcaggccttg atggctttca atacattaat catttcaagc gcggtcagtg
    10561 cagcttctgc acctttgttg ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc
    10621 tttcagtggt cagaacacca aaagcaaccg gaatttcgct gtcctgggca acatgcgcca
    10681 ggccgttgct tgcaccacca gcgacatatt caaagtgggc agtgccacca cgaataaccg
    10741 tacccagcgc aatcaccgcg tcgtatttac cggttttagc cagtgcaccc gccgccagcg
    10801 gcagctcata ggcacctggc acccaaacaa cggtaatgtt ttcatctttt acctgaccga
    10861 tacgtttcag tgcgtcaatt gcaccttcca gcaggctgtc attgataaag ttgttgaaac
    10921 gcgcgatggt gatggcgacg cgagcgtccg gggtagcaac gttagcttca ataatgttca
    10981 ttttcttcct tcgggttcga gtatggcccc gcaggggggc ggattttagc ataatatttc
    11041 gtgcgctgct tccctttcga gccgggagat catgcaccca ctaaatgcag gcaaacatcc
    11101 gggcctacat gacgtatctc tttgaattta aattggggag cgtcggctaa tttctcaagc
    11161 cctggcagcg tgcataatcc acgggcgtcg ttgcctaata gtttaggtgc gatatagaca
    11221 atcagctcat cgactaaacc cgcctgtagc aatgcgccag cgagcgttgg ccccgcttcc
    11281 acccagatgc tgttaatctg ctgtttaccc agttgcatca ttagtacaac cagatccagg
    11341 tgacctttat gctctggaat cagcaaggta cgcaccgttt ccggccactc acgagaatct
    11401 tcctgcgtac gcgcgaacca ggtttcgccg ggctgctgca caatgcgatg ttccggcgtc
    11461 acgcgatttt ggctatcaat cacaatacgt accggctggc ggagattttg ttgcgggtag
    11521 agcccctgag tttgttcatc cagttcagac caacgcactg ttaaggctgg atcatccgcc
    11581 agcaccgtgg cgctgctggt taaaatggca tgactttgcg cgcgcagtcg ttgtacgtcg
    11641 cgtcgcgcct gaggcgaagt gatccactgg ctttcgccac tcgccatcgc cgtgcgacca
    11701 tcaagcgatg cgccaagttt taactgaata taaggaaagc cggtgcgcat ccgtttgaga
    11761 aagcctttat tcaattgctc agcttcactc atcatcagcc cgtggctgac gtcaatgcca
    11821 gcctgttgca gacggtaaag tccacgtcca gcgacctgcg gattggggtc ttgcattgca
    11881 gcaaccacgc gcgccacgcc cgcagcaatt aatgcatcac aacacggtgg cgtacgacca
    11941 tgatggctac agggttcgag tgtgacatac gccgttgcgc ctttggcttt ttcgccggcc
    12001 atgcgcaacg cgtgcacctc ggcatgcggt tcacccgctc gttgatggta accttcaccg
    12061 acaatttcgc catctttgac aatgacgcac ccgacattcg ggttgggatg cgtggtaaaa
    12121 cgtcctcgtt gcgccagctt tagcgcccgc gccatgtaat actcgtcctg cacggcttag
    12181 tcctccaggc gcgcgatctc ttcgccaaat tctttgatat cttcgaaact gcggtagaca
    12241 gaggcaaaac ggatataggc gactttatcg agctttttca attgctccat caccagattg
    12301 ccaatcatct tgctcggcac ttcgcgctca ccggtggcgc gcagctgcga ttttatatga
    12361 ttgattgcca tttcgacgtc atcggaactc actggacgtt tttccagcgc ccgcagcatt
    12421 ccgctacgta atttctcttc attaaacggt tcacgcacgt cgttgctttt tacaacacgc
    12481 ggcataacca gctccgccac ttcaaaggtg gtgaaacgtt cattacacac cagacactgc
    12541 cgacggcggc gtacggatga accctcgccc acgagacgag agtcaattac cttagtgtcc
    12601 acggcgaaac agaatgggca atgcatacgg cgtcctgacc aggtggttaa cagaaatcta
    12661 ttttaccctg aactgacgat acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc
    12721 tttcactgct tttaccctct accattaggc taattgcgac gagaaaggga cgacctatga
    12781 atactaacgt ttttcgactg ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc
    12841 agcaaagtga agtgcgacag ataaaacaca gcgtcagcac gctgaaccag gagatgacgc
    12901 agctcaataa agaaacggtc aaaatcactc agcaaaacaa gctgaatgca aaatccagca
    12961 gtggggttta ccttctgcct ggagcaaaaa caccagcaag actggaaagc cagatcggta
    13021 ctttacgtat gtcgctggtg aatattacgc ctgacacaga tggcactacc ctgacgctac
    13081 gtattcaggg cgaatccaat gaccctttac ccgcattcag cggaaccgtt gaatatgggc
    13141 aaattcaggg aacaatagac aactttcagg aaatcaatgt gcagaatcaa ttgattaacg
    13201 cacctgccag cgtcctcgcc cccagcgatg ttgatattcc gttacagtta aagggtatgt
    13261 ctgtggatca gttaggcttt gtacgtatcc acgacattca acctgttatg cagtaaacgt
    13321 attgcggggc gatattgtgc gtcccgcaac atctttttcc gtcattttgt tactccgctt
    13381 acatcacctg gattgatagt aaaagtttgc aacaagggtg aaagtcagta caatccccgc
    13441 ccgaatgtgt gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa
    13501 catatttttg tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa
    13561 cgcaatttgc gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa
    13621 aaaacattac tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca
    13681 gctgaaaacg acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc
    13741 ggaagctatc acacccgttt cggaccgcag ctccgcaacg atacctacct tgagtacgaa
    13801 gcattcgcta aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc
    13861 ggcggtaact ccgatgcaaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc
    13921 gaaccgcgtt tctccatcga caagctgacc aatactgacc ttagctttgg tccgttcaaa
    13981 gagtggtact tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggccgccag
    14041 agcacctggt acatgggtct gggtaccgac atcgatactg gtctgccgat gagcctgtcc
    14101 atgaacgtct atgcgaaata ccagtggcag aactacggcg cagcgaacga aaacgagtgg
    14161 gacggttacc gtttcaaagt gaagtacttt gtgccgatta ccgatctgtg gggcggccag
    14221 ctgagctaca tcggcttcac caacttcgac tggggttccg acttgggcga tgacagcggt
    14281 tatgcgaaca acggtattaa aacacgtacc aataactcta ttgcgtccag ccatattctg
    14341 gcgctgaatt acgatcactg gcactactct gtcgttgccc gttactggca taacggtggt
    14401 cagtggaatg acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc
    14461 ggctggggtg gttatctggt agtaggttac aacttctaat tgtgagaatg ccggatttca
    14521 tatccggcat tttttatcac ttcttcttgt tcatcatcgc tttcaaatca gcgaatgggt
    14581 tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat
    14641 actgatccgc ttcggtgtat ttcgaatgct catgatcgtg gcaatagaga caaagtaatt
    14701 cccagttact gccatcttcc gggttattag tatggtcgtg atcaatgtgg tgaacggtaa
    14761 gttcacgcag gttggaataa acaaactcgc gggaacagcg accgcagacc cacggataga
    14821 tttttaatgc tttttcgcga tagccgcttt ctaaccgcgc atagtttttt gggatgatag
    14881 ccatgaaaaa gttacctgga tgaaatgtag ggttacgcgc agtttcccaa gatgcgggga
    14941 ttttcgcaat caccttacat caaaaaaaat cccgatcttc tgaccgggat tttcaacatc
    15001 agctgaaact tgattaaggc agaattgacg gctgatccgc cccttctttt tccactttct
    15061 gctgcaacat gtgttcgcgc ttcataccca gtttcagagc cagcgcagat gccacataga
    15121 tggaagatgc agtaccgatg gaaacaccga taagcatggt cagcgagaag ccttccagta
    15181 ccggaccacc gaagaggtac agcatcagga taaccatcaa ggtagtaccg gatgtgatca
    15241 aggtacggtg cagcgtctgg gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac
    15301 ggatcttgcg gaagttttca cgaatacggt ccgataccac gatactgtcg ttaagcgagt
    15361 aaccgataac cgacatcaac gatgccacaa tggtcaggtc aatctcgata tggaataacg
    15421 acaaaatacc cagcgtaatg ataacgtcgt gcgccagcgc aataacaacc cctgccgcca
    15481 gtcgccactc aaagcggaaa cctacgtaca cgaggataga cagcagcgcc gccatcaacg
    15541 ccatcgcacc ggtttgcgca aggtctgccc ccacgctcgg accgacgaac tcaatacgct
    15601 tcactgccgc attctgattg gtggattcgt taatcacctt cagaacctgg ctgcccaaca
    15661 cctgaccgcc ggtttcgcct tcagcaggcg gcatacggac catgatgtcg tggctgctac
    15721 caaagttttg cagcatcggc tcttcaaaac cggctttttg caatgcatca cgcattacgt
    15781 caatttcagc cggtttttcg agcgtaattt caataaccgt accaccggta aaatccagcc
    15841 cccagttaaa gccgcgcacg cccataataa cgatagcggc gattaacagc agaccagaga
    15901 tgccgaaagc ccagtagtcc cagcgcataa agtcatagac tttacggccg tggtttagtt
    15961 gttcaacagt atattcctgt gccacatcgc actcctcaga ttgacagctt cttgacgcgc
    16021 ttgccgccat atagcaggtt tacgatggca cgcgtaccga caatcgcggt aaacatcgac
    16081 gtcgccacac cgataccggt agtaatcgcg aaccctttaa ttgccccggt acccactgca
    16141 tacaggatga tgactttaat cagcgtggtg atgttcgcat cgaagatgga actgaatgcg
    16201 ccacgataac cttcatcaat tgcctgttga acagtacgtc cgttgctcaa ctcttcttta
    16261 atacgttcgt tgatcagtac gttcgcatcg accgctaccg caagggttaa gacgataccc
    16321 gcgatacctg gcatactcag cgttgcgcct ggcaacagcg acataatacc gacaatcaag
    16381 atcaagttgg caatcagagc actggtcgca atcagaccaa acttcttata gaagatgatc
    16441 atgaacagaa tagaaaccag cagaccggca aggcaagcct ccagcccctg ctcaatgttc
    16501 tgcataccca gggttgggcc aatggtacgt tcttcaacaa tctgaatcgg cgcgatcaac
    16561 gcaccggcac gcagcagcag tgaaagctga cgcgcttcgc ttgggttatt gatgccagta
    16621 atacggaagc tgttacccag acgagactgg atgttggcga tattaatcac ctcttcctgt
    16681 ttcaccagaa ccgcacgacc gtttgcatct ttcttaccgc tgtctttgta ctccacaaac
    16741 agggttgcca tcggtttgcc gatgttgtcc ttggtgaagt tagacatgat gttaccaccc
    16801 gcgctatcga gcgagatgtt aacctgcggc tggttgtatt cgtcctggct ggaagtggag
    16861 tcggtgatat ggtcaccggt cagaattacg cgtttgtaca gcacaaccgg ctgaccttcg
    16921 cgggtctgtt tcacttcaga gtcgcccggt acgcgaccgg atgccgcagc ggcctgatca
    16981 acgttggtgt ttaccagacg gaattccagc gttgccgtcg cacccagaat ctctttcgca
    17041 cgcgcagtgt cctgaatacc tggcagttca acaactatac ggtcagcacc ctgacgctga
    17101 accaccggct ccgccacgcc aagttggttt acacggttac gcaggatatt gatgttctgc
    17161 tgcaccgcat attcacgcgc ttcactcaga cgagcatcgc tcattaccgc acgcagctgg
    17221 ttgctgccct ggctgctaat caccaggtcc ggatggcgct tgctcagata cgcaatggct
    17281 tcatcacgag ctttagcatc gcggaaagtg atgctcaggc cgtagttgtt ttctttacga
    17341 acggtggtat acgggatgcc cttttcacgc aggtcgctgc gcaggctatc gatattttgt
    17401 tcctggagtt tgccaagcgc ggtgtccata tccacttcca tcaggaagtg aacgccgcca
    17461 cgcaggtcaa ggccgagctt catcggctca gcatgaatag ctgccagcca gcgcggcgtt
    17521 gccggggcaa ggttaagcgc cacgacgtat ttgtcaccca taacgcccat taatgcttca
    17581 cgagcgcgca gctgggtgtc agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt
    17641 gccacagact tagcagttat tttttcttct tgtaacgttt tctggacctg gatcagcgtt
    17701 tgctcactgg cggcgactcc gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac
    17761 aggttgggaa gcgcatacag cagaccgatg acaatcacca cgatcagcat gacgtacttc
    17821 cacaaaggat aacggtttaa cacggcaatt cccttaggga aaaattttaa ttacagcgcc
    17881 ttcatggtgc ctttcggcag aacggcagct acgaagtcac gtttaataac tacttcagtg
    17941 gtgtcattca gtgcgatagc aatgtagccg ttttccgcta ctttggttac gcgaccgacc
    18001 aggccaccgt tcgtcagcac ttcatcacct ttagcgatgg agtccatcag ctttttgtgt
    18061 tctttggtgc gcttctgctg tggacgcagg atcatgaaat agaaaatcag accgaatacc
    18121 accagcatca aaatcaaaga catcgggcta ccttgcgccg gtgcccccgt tgccgctacc
    18181 gcatcagaaa taaaaaagct cattaaattt ccctcattat taatattaat caacgttcaa
    18241 aggtggtact tctcgcccct gacgctggta aaaatcagtt acgaagctct ctaatttacc
    18301 ctcttcaata gccttgcgta aacccgccat caaacgctgg tagtaacgaa ggttatgaat
    18361 ggtgttgagt cgcgcgccta atatttcgtt gcaacggtcg agatgatgca agtaagcgcg
    18421 tgaataattg cgacaggtgt agcaatcaca ctcaggatcg agtgggccag tatcgctctt
    18481 atacttcgca ttgcggattt tcaccacgcc atcggtcacg aacaaatgac catttcgggc
    18541 gttgcgggtt ggcattacgc agtcaaacat atcgataccg cgacgtacgc cctcaaccag
    18601 gtcttctggt ttaccaacgc ccatcaggta acgcggtttg tctgccggaa tttgcgggca
    18661 tacatgctcc agaatgcggt gcatatctgc tttcggctca cccacagcca gaccgccgac
    18721 agcgtagcca tcaaaaccga tatctaccag acctttaaca gaaatatcac gtaaatcttc
    18781 gtaaacactg ccctgaatga taccaaacag cgcatttttg tttccgagac tgtcaaaacg
    18841 ctcacggcta cgcttcgccc aacgcagaga catctccatg gagcgttttg cgtaatccca
    18901 gtcagcagga tacggcgtac actcatcaaa gatcatgacg atatccgaac caagatcgta
    18961 ctgaatctcc atcgactttt ccgggtcgag gaaaatcggg tcgccgttga tcgggttgcg
    19021 gaagtgaacg ccctgttcgg tgattttacg aatatcacca aggctgaaga cctggaagcc
    19081 gccggagtcg gtaaggattg gtcctttcca ctgcataaaa tcgtgcagat cgccgtgcag
    19141 tttcatgatt tcctggcccg ggcgcagcca caggtggaag gtgttgccga ggataatttg
    19201 cgcgccagtg gcttcaactt cttccggcgt catccctttt acggtgccgt aagtgccaac
    19261 aggcataaaa caaggcgttt ccactacgcc acgatcaaag accaggcggc cacggcgtgc
    19321 gcgaccgtcg gtggtgtcca gttcaaattt cattttttct ccactacgtc agaaaaacag
    19381 tccaacgttt aaaccagcgc cgcggaatta ctccccaacg cgctcattaa ttgcctgcgg
    19441 attgtacgtg ataaacatcg catcaccgta actaaaaaag cgatatttct cttctaccgc
    19501 cgctttatag gcattcatgg tgtgttgata accagcaaat gccgaaacca gcataatcag
    19561 cgtcgattcc ggaaggtgga agttcgtcac cagcgcatcg accactttgt actggaagcc
    19621 cggatagata aagatttggg tatcgtcaaa gaacggttca atgagatcgt ttttcgctgc
    19681 ctgagccgca ctttccagcg aacgtactga ggtggtacca accgcaatca cccggttacc
    19741 gcgcgctttc gccgccagta ccgcgtctac cacatcctgc ggcacttcag cgtattccga
    19801 gtgcatgatg tgatcttcaa tggtgtcgac gcgcaccggc tggaaggtgc ccgcaccaac
    19861 gtgcaacgtc acaaacgcca tctccacgcc tttggcgcgt aatttttcca gcaaaggttc
    19921 gtcaaagtgc agtccggcgg tcggtgctgc taccgcgccg ggtttttcgc tataaaccgt
    19981 ttgataaagt tcgcggtctg catcttcgtc cggacggtcg atgtacggcg gcagcggcat
    20041 atggccgatg ctgttgagaa tatccagcac cgaacgttga tcattaaatt cgacttcaaa
    20101 cagtgcgccg tggcgcgcgg tcattgttgc gttaatactt tcgtcatcgc ccagcagcag
    20161 ttctgcgcca ggttttggcg ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc
    20221 gagcatccgc tcaaccagca cttcaatctt gccgccgctg gctttacgcc caaacagacg
    20281 cgccgggatc acgcgggtat tattaaaaac cagaagatcg ccggggttga gcttatcgag
    20341 taaatcggtg aaagtaccgt gcgtcagcgc gcccgtcggc ccgtccagcg acagtaaacg
    20401 acagctactg cgttcaggca tgggatagtg ggcaatcagg gattcgggca attcaaaaga
    20461 gaaatcggta acgcgcatga cactgactca gactaaaata agaggcgggt agtctagtgc
    20521 cggggcgctc tccctgcaac ctttaccccc ctttggatag aatataatcg atgaattttt
    20581 tagctcacct gcatttagcc catctcgcgg aaagctcgct ttccggcaat ttactggctg
    20641 atttcgtacg cggaaatcct gaagaaagtt ttccgcccga cgtcgtggct ggcattcata
    20701 tgcatcggcg aatcgacgta ttaaccgaca atctgccaga agtccgcgaa gcgcgggagt
    20761 ggtttcgtaa tgaaacgcgc cgtgtcgcgc ctattacgct ggatgtgatg tgggatcact
    20821 ttctgtcccg ccactggtcg cagctgtcgc ccgattttcc gctacaggaa tttacctgtt
    20881 atgcccggga gcaagtgatg actattcttc cagactcacc gccacgcttt atcaatctga
    20941 acaactactt atggtcagag cggtggttgg tgcgctatcg cgatatggat ttcatccaga
    21001 gcgtgttaaa cggcatggca agccgtcgcc cacgtctgga tgccctgcgt gactcctggt
    21061 acgatttaga cgcccattac gatgctctcg aaacccgctt ctggcagttt tatccgcgga
    21121 tgatggagca ggcgtcacgc aaggcgttat aagacgttaa ttcatccaca ccgtagccga
    21181 aatggcaggc aaagccagaa tcccgtccgt cagttgcccg tgaccttctt tgcgttgcca
    21241 ttgcgcgaca ttaagcaacg gcgacgcggg tagcaccact tcacaggctt cgccacggtt
    21301 gattgccacc agtacacgct gctgattcag cacgcggaca aataccacca cgttatcttc
    21361 cgcatacagc acctgacagc cgccacgacg caacgcctga cttttcttac gcagcgcaat
    21421 cattcgctgg tacagcgcga ataacgccga atcctgcttt tccacctgcc aggggaacgg
    21481 cttacggcaa aacggatcgt ttttgccatc cagtcccact tcatcaccgt aataaatgca
    21541 cggtacacca ggccaggtga acagccagac caccgccagg ggcaggcgcg caacatcccg
    21601 accgagcagc gttttaaatc gcgcagtatc gtggctgtcg agctgattaa acatacgtaa
    21661 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    21721 gtcaatttgc tgcggatcgt aagagatatc ggtgttggca agaaatcccc acaacgggaa
    21781 tgtgaagccg cgatagttca tggcggcatc ttccacatcg gcctgtaacc attgccgtgc
    21841 atcgccaaaa tgttcgccga caatatacgc atccggctgg gtttctttcg ccgcttcggt
    21901 gatcccggca acgtgctgca aattattgcg caccccaccc gcttccccca gcatatgcac
    21961 cacatccagc cgccagccat ccatattcca cggcgctttc agccagtggc ggacaatact
    22021 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcggcaa
    22081 gctggcatag cccagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcacg
    22141 ccagggtgat tcggggttgt gacaagctcc ccccgtgcca cgattgtgcc tgtcaaacca
    22201 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagtcgca ttcccagctg
    22261 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    22321 gcgataatcc tcggtatcgt atttatgcac gctgggagct ttaaacaccg gattgagata
    22381 cagcgccgtc acgccaagtt ttttcagata cggcagtttt tcacttatcc cgtccagatc
    22441 gccgccatag aacgttgatc cgccagcctg cgccgtgacc ggttcatccc agtcacgcaa
    22501 tatgatctct tgtccggctg cgtgatggta atagacatgc tcctgttcag cttcacgagg
    22561 aagattacgg gcgaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    22621 tgggccgata tccggtacat caatggcaaa ctgctccagt cgtgctggcg gcattcggct
    22681 gaagccttgc ggtgtaaacc aacgctggcg atcgtgccac agcaatttga aactataacg
    22741 ccgccgaggt tgtccgcagg agagctcgat cgccgcccgc catgcggtga cgacaggctg
    22801 cggctgactg cgctgcttat gcatcggtac tgacatttct tcgttatcgt gttctgtacg
    22861 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    22921 tttgctttgt ttaacaaatg ggggcaccgg cagatgccat gcatttaaca tcataattcc
    22981 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    23041 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    23101 ctcctgaacc ggcttacaga acgcaaggat tactggtttt cagccagctg acgatcgtgg
    23161 cggcgcttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    23221 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag aaaaatcaaa
    23281 ccgccgatgg tggttgctac cccacccggt actttaaatt tcagcgcctt aacttcttct
    23341 ggcggtaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccacacc
    23401 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    23461 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    23521 cgacgcgagg ttttgctgaa aatttttggc gcgctgccct gctctgccat accgtggagc
    23581 atacggccta caccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    23641 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag cacaaacggg
    23701 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    23761 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    23821 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    23881 accatttgca acgacatcac cataccaagc cagccgttac tgaagaagcc gccgttgctc
    23941 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    24001 aaaccggcga caatcatgat gatgatagtg gcgactttaa agaacgagaa ccagaactcc
    24061 agctcaccga ataccttcac gctcatcagg tttacggcgc aaatgatcag caccacactc
    24121 agtacccaaa tccagtgcgg caccgtcggg aaccagacac ccatatagat accaaaagcg
    24181 gtcacatcgg cgatggcgac aataaggatt tcaaagcagt aggtccagcc ggtaatgtaa
    24241 cctgccagcg ggccgaggtt ttcctgcgca taacgcgaga aagagctggc tgccgggtta
    24301 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    24361 taggccaaca acacgcttgg accggccatt ttgatggcat ctgccgaacc gtaaaacagc
    24421 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt gtcgggtact tagcccacgc
    24481 tttagcttgt tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    24541 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    24601 tgacgacctg ccgcacgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    24661 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    24721 ctgaatgcag atgttttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    24781 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    24841 gcgatacacg gcggataaat ggcggtcagc accggtacag agatctgaat cagctggctc
    24901 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    24961 gagagcggta cgtactgggc gaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    25021 accaggcagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggta
    25081 tgctgaacgt aagcatgcag gatcgccgca ccgtttgcag actgatcgac cagcgacgcg
    25141 ctgtctgaac ccagacggaa cagcgccagg tacagcagag tcagaccaac acccgccatc
    25201 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    25261 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    25321 ccgttgacga agccgttaga aaacgcagcg ttttgataag cctcagtcgc cgtgctgata
    25381 gaacccgccg gccagataat tgcggcaaca gacaggatga ccagcgcgat aattttcagc
    25441 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    25501 acgatagcga aatagaccag gctgtaaata aacagcggca gcgcggaatc acccgtcagc
    25561 ggcgcaatgc ccacttcaaa agaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    25621 accgccaggt aacaaactgt tgccagcagt acgccagcga ctttaccaat tggcgtgctg
    25681 agactgtcaa caccgccgcc aacttttgcc agtgccacta ccgttaatac cggtaggcca
    25741 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgcaagccg
    25801 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    25861 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    25921 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    25981 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    26041 cgtaaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    26101 agcaagaagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    26161 aacactattt caatgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccattg
    26221 taaaatctaa cgcccgtatt tcgactgcaa agaaaataag ccagcatcgg ctggcttatg
    26281 gcaataaaag atgacaaagg cgaattaatc gctgtttttg gcaattaaac gttccgggat
    26341 aacaaaactg aaacgtgtac cttttcctac tgtactctca atattcaggc gactttcgtg
    26401 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccaccgg tttgccggga
    26461 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    26521 gcccggtccg ttatcttcaa cactaaattc ggcaccgtgc ggcactcgct gccagcgtac
    26581 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    26641 actgcgtagc tgatcttcgt tgcctgacac cttaaggccg ttatctatct caaaggtaaa
    26701 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acgacgcgca gcatcatcgg
    26761 cacatcaacc ttttcattga gcaaatgcgt cggtgcggct tctattttcg acagcgtcag
    26821 caattgcttc accaaccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    26881 ttcacgtacc gcgccttcca gcggttgctc atccatcatc tccaggtaac cctgtaacac
    26941 ggtcaatggc gtacgtaact catggctcac attggcaaaa aagttacgcc gcgccccttc
    27001 cagttgatgc atttgcgtga catctcgcgc caccatcagc aactgtttgt gggtataagg
    27061 catgacgcga atctccagat gccgcccggt gttgagcacc agattgagcg ggcgagagaa
    27121 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agcaagttaa ggatgttctg
    27181 cccgttatct tccggccagc gcaagccaag aatctgttgc gccagaccgt tgcaccagaa
    27241 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    27301 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    27361 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactacg
    27421 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    27481 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    27541 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    27601 gtaagatact ccagttaaga aatcataagc cctgctctac gtccgatgag caaggcctta
    27661 aaagcgggtt gaaaaacgat accctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    27721 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtac gatcttccac
    27781 atacacgttg gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    27841 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggttcttc
    27901 gcccgccatc acccggtgag atgtcgggtc cagacttagc ccctgcatct cgatcacctc
    27961 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    28021 cggagaaaac ggcttggtga tatagtcatc cgcaccggtt tcaaggccgc gcacgcgatc
    28081 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    28141 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt cgaggagaat
    28201 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    28261 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    28321 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    28381 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    28441 tcctttcagg ttattgattt ccgtggcaca gaaaaaagca aatggcacat ctgtttgggt
    28501 ataatcgcgc ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    28561 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    28621 gactggctgc tggagacagc acaagcccat caggtggatg cgattattgt tgccggtgat
    28681 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    28741 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    28801 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata ctaccgtggt cgccagcgcc
    28861 ggacatgcgc cgcaaatatt gccccgtcgc gacgggacgc caggcgcagt gctgtgtccc
    28921 ataccttttt tacgcccgcg tgacattatt accagccagg cggggcttaa cggtattgaa
    28981 aaacagcagc atttgctggc ggctattact gattattatc aacaacatta tgccgatgcc
    29041 tgcaaactgc gcggcgatca gcctctgccc atcatcacca ctggacattt aacgaccgtg
    29101 ggtgccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    29161 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcgcagatt
    29221 attggcggca tggaacatgt tcgctattgc ggttccccca taccactgag ttttgatgaa
    29281 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    29341 gaaaacctga acgtaccggt aacgcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    29401 attaccgcac agctggaaca gtggcgcgat gtatcacagg agccgcctgt ctggctggat
    29461 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    29521 gaatcattgc ctgtcgaagt attgctggta cgccggagtc gtgaacagcg cgagcgtgtg
    29581 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    29641 cgtctggcac tggaagaact ggatgaatca cagcagcaac gtctgcagca tcttttcgcc
    29701 acgacgttgc atagcctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    29761 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    29821 gcaacgggct gtttgctatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    29881 ccatttgtct ggcgctgtat cacgaaaccc cgcgcctctc taacgtttca caatcgcaaa
    29941 atgatctcat gacccgcgat accgccgaat gtctggcgga agtggagttt gaagtcaaag
    30001 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    30061 tgcaggtgcc acgcgtagag ctggcgcgct gtgcagacgg caaaattctc gccgacaaag
    30121 tgaaagataa gctggaactg acggcgacgt taaccggact ggattacggg cgcttcaccc
    30181 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    30241 gcgcagaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    30301 tttttgagca gcacaaatcg gcccgcacag agctggagaa gctgcaagcg caggccagcg
    30361 gcgtcgcgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    30421 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    30481 taacgcgtct ggacgaattg cagcaagaag ccagccgccg tcaacaggcc ttgcaacagg
    30541 cattagccga agaagaaaaa gcgcaacctc aactggcggc gctcagtctg gcacaaccgg
    30601 cacgaaatct tcgtccgcac tgggaacgca tcgcagaaca cagcacggcg ctggcgcata
    30661 ctcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgtgcga
    30721 gcattcgcca ccacgcggag aagcagtcag cagaattaca gcagcagcaa caaagcctga
    30781 atgcctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    30841 gtgcacagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    30901 taactcatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    30961 atgaagttgc tagcgccctg gcgcaacatg ctgagcaacg cccactgcgt cagcgcctgg
    31021 tcgcactgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta caggtcgcta
    31081 tccagaatgt cactctggaa cagacgcaac gtaatgccgc acttaacgaa atgcgccatc
    31141 gttataaaga aaaaacgcaa caacttgccg atgtgaaaac catttgcgag caggaagcgc
    31201 gcattaaaac gctggaagcc cagcgcgcac agttacaggc gggtcagcct tgtccacttt
    31261 gtggttccag cagccacccg gcggtcgagg cgtatcaggc gctggaaccg ggcgttaatc
    31321 aggcccggct attaacgctg gaaaaagaag tgaaaaagct cggcgaagaa ggtgcggcgc
    31381 tacgtgggca actggattca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    31441 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    31501 atatcaccct gcagccacag gacgatattc aaccgtggct agatgcacaa gatgagcacg
    31561 aacgccagct gcggttactc agccaacggc atgaattaca agggcagatt gccgcgcata
    31621 accagcaaat tatccagtat caacagcaaa ttgaacaacg ccagcagcaa ctcttaacgg
    31681 cattggcgag ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    31741 cgcgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    31801 accgtatgca gcagctgacg ccgattctgg aaacgttgcc gcaaagtgat gaactcccgc
    31861 acagcgaaga aactgtggcg ctggaaaact ggcggcaggt tcatgaacaa tgtctcgcat
    31921 tacacagcca gcagcagacc ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    31981 aagcccaggc gcagtttgac accgcgctac aggccagtgt ctttgacgat caacaggcgt
    32041 tccttgcggc gctaatggat gaacaaacac taacgcaact ggaacagctc aagcagaatc
    32101 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa gcgctggcac
    32161 tgcatcagca acaccgacct gacggcctgg atctcagtgt gacggtggag cagattcagc
    32221 aagagttagc gcaaactcag caaaagttgc gtgaaaatac caccagtcag ggcgagattc
    32281 gccagcagct gaagcaggat gcagacaacc gtcagcaaca acaaacctta atgcagcaaa
    32341 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    32401 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    32461 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    32521 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    32581 ccctttccgg cggcgaaagt ttcctcgtca gtctggcgct ggcgctggcg ctttcggatc
    32641 tggtcagcca taaaacgcgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    32701 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaatgcc agtggcaaaa
    32761 ccatcggtgt gattagccac gttgaagcga tgaaagagcg tattccggtg cagatcaaag
    32821 tgaagaagat caatggactg ggctacagca aactggaaag tgcgtttgca gtgaaataac
    32881 tattcagcag gataatgaat acagaggggc gaattatctc ttggcctcgc tggtcgttat
    32941 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    33001 tggtatgaaa aaagttattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    33061 atttggcatt atgggcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    33121 cgccgggcat atgatctcgt attatgcgct gggggtggtg gtcggtgcgc caatcatcgc
    33181 gcttttttcc agtcgctact cactcaaaca tatcttattg tttctggtgg cgttgtgcgt
    33241 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    33301 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    33361 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    33421 caatttgctg ggcattccac tgggaacgta tttaagtcag gaatttagct ggcgctacac
    33481 ctttttgttg atcgctgttt ttaatattgc ggtgatggca tcggtctatt tttgggtgcc
    33541 ggatattcgc gacgaggcga aaggaaaact acgcgaacaa tttcactttt tacgcagccc
    33601 tgccccgtgg ttaattttcg ccgctacgat gtttggcaac gcaggtgtgt ttgcctggtt
    33661 cagctacgta aagccataca tgatgtttat ttccggtttt tcggaaacgg cgatgacctt
    33721 tattatgatg ttagttgggc tagggatggt gctggggaat atgctaagtg gcaggatttc
    33781 aggacgttat tcaccactgc gcattgcagc agtgactgac tttatcattg tactggcact
    33841 gctgatgctc tttttcttcg gcggtatgaa aacaacgtcg cttatttttg cttttatttg
    33901 ttgcgcggga ttatttgccc tatcagcacc tctacaaata ttattactgc aaaatgccaa
    33961 aggcggagag ttattaggtg ccgcaggtgg gcaaatagcg tttaacctcg gtagcgccgt
    34021 cggcgcatat tgcggtggta tgatgctgac gctggggctg gcatataatt acgtggcgct
    34081 gcccgccgcc ctgctttcgt ttgctgcgat gtcgtcgttg ctgctgtatg gtcgctataa
    34141 gcgccagcaa gcggcggata gttcggtgct ggcgaaacca ctggggtaga tagcagtcgc
    34201 agtgcgcatc gcttgttgcc ggatgcggcg tgaacgcctt atccggccta cgggactgtt
    34261 cattttgtag gcctgataag acacgccagt gtcgcatcag gcatcggagc accggggggt
    34321 tgccgccact gtgcgcgtta tttgttgccg gatgcggcgc gagcgcctta tccggcctac
    34381 cggacggctc actttgtagg cctgataaga cgcggcaagc gtcgcatcag gcatgatacg
    34441 ccaattgcct ggttttttta ctcttgtggc cataaccatg cagcgccgcg tacgccgctc
    34501 gaatcaccat gtttcgcctt gcgtaccggc gtttcacatt ctccgccgaa gacaaattgt
    34561 ttaatcaact gtggaaccgt ttgatataaa cggtctacat tgctcattcc gccgcccagg
    34621 acaatcacat ccggatcgag aatattcacg acatgtgcca gcgattttgc cagccgcagc
    34681 tcgtagcgac gcaatgccag ttccgctacc ggatcgcttt cttcaaccag gcggataatt
    34741 tcactgcctt tcagcgcatg tccgctcaaa cgacgataat ccgtcgcgaa tcccgtgcct
    34801 gaaataaagg tttcaataca accttgttta ccgcaataac aagggacttc ctcgcgataa
    34861 cgcagttcgt cttcgtccat ccacggtagc ggattgtgtc cccactcgcc tgccgtgcca
    34921 ttgccgccga tatgcgcccg cccattgaat gccacgcccg cgccgcatcc cgtgccgata
    34981 atcacggcaa ataccgtctg cgctcccgct gccgcgccat ctaccgcttc tgaaaccgcc
    35041 agacagttgg cgtcatttgc cagccgcact tcccgctgca accgcgcgct taagtcttta
    35101 tcgaatggct gaccgttgag ccaggttgaa ttggcattct tcaccacgcc ggtgtagggc
    35161 gaaatcgaac caggaatgcc catacctacc gttccgcgct gccctgttgc ctgctccgcc
    35221 atatcaacca acgtggcgat cgtttcaata gtctgccggt aatcatcacg cggcgtgggc
    35281 agacgatggc ggtacaactg ctcccctgca tcgcccaatg caatcacttc agttttggtg
    35341 ccgcctaaat cgatacctat acgcacggta ctctccttat ttttttcaat atcaatagcg
    35401 tagagacaga caaccggatt ggcaatgcaa ggccgccgac aattcgttat catgcccgct
    35461 aaatttaacg acaaggccgt ggaaattatc atgctgtggt tcaaaaattt aatggtttac
    35521 cgtcttagcc gcgagatttc gctgcgtgca gaagagatgg aaaaacagct agcctcgatg
    35581 gcatttaccc catgcggcag ccaggacatg gcgaagatgg gctgggtacc tccgatggga
    35641 tcgcacagcg atgcgttaac gcacgttgcc aatggtcaaa ttgttatctg tgcgcgcaaa
    35701 gaagaaaaaa tcctcccgtc cccggtgatt aaacaggcgc tggaagcgaa aatcgccaaa
    35761 ctggaagcgg aacaggcgcg taagctgaag aaaaccgaaa aagattcgct gaaagacgaa
    35821 gtgctgcact ctctgctgcc gcgtgctttc agccgtttta gccagacaat gatgtggatc
    35881 gacacggtta acggtttgat tatggtggac tgcgccagcg ccaaaaaagc ggaagatacg
    35941 ctggcactgc tgcgtaaaag cctggggtcg ttaccggttg taccattgag catggaaaac
    36001 ccgattgaac tgacgctgac cgaatgggta cgttccggca gcacggcaca gggcttccag
    36061 ctgcttgatg aagccgagct gaaatcgttg ctggaagatg gcggcgtgat ccgcgcgaag
    36121 aaacaagatc tgaccagcga agagatcacc aaccacattg aagccggaaa agtggtgact
    36181 aaactggcgc ttgactggca gcagcgcatt cagtttgtga tgtgcgacga tggttcgctc
    36241 aagcgtctga agttctgcga cgagctgcgc gatcaaaacg aagatatcga ccgtgaagat
    36301 ttcgcccagc gctttgatgc cgatttcatc ctgatgactg gtgaactggc agcgttaatt
    36361 caaaacctga ttgaaggatt aggtggcgaa gcacaacgtt aattgctgat ttacctttaa
    36421 tgccggatgc gacgcctgcc gcgtcttatc cggcgtacga agccacacca ggaatataat
    36481 cgttagctac agcgggcaat aattcttagc gcagcaatat tatgcgtttt atgctgtaac
    36541 ttgctccagg ggcgttgtgt cattgttttt cctcaagccg gtgacgcgct tcactgcgaa
    36601 tttttcgcgt tgccccgttt ttcactaaat cacgtaacag cgaaaaatcg ggttgttcga
    36661 caaataagag taatgatggc tcctctttta accacgtagt tttcacatcc gcagccagct
    36721 gtgaattatt aatagccagc gaccgctcct gaggctcagt caatgtcgtt aacaacaccg
    36781 gagtatgtgc gttgtgcaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg
    36841 cacaccacca ttcatcatct gatttatctt ttaacgtcgc cagttccgca acaatatctg
    36901 gagatcttcg ctgatattta tatcaacctt gatgaattat gaatatatcg aaaatcgaaa
    36961 aaatcccagc aaaggaaata ttattatggg ttaaagagag gacatttcag aaaaatgccc
    37021 tcccgttcaa ggggaaggcg aggaattaca gatagcggca cagataagag gtgggttcgg
    37081 caacttgcag atgaaacttg ctgtgaccgg gaacattaaa caccgaaccg gcttcataca
    37141 cttgccagtc ggtcgcgtca ggcagtaaca cattcagcgc gccactgatt accgtcatct
    37201 cttcaggctc agcggtgctg aaggtgtatt caccttcaac cataacaccc acgctggcgc
    37261 gaccagtgct gctgctggaa aagccgatcg atttcacttt gccggaaaag tactcattac
    37321 tttgaagcat aaactggccc tgtctggtag ttgaacatct acttcactat aggggccaga
    37381 ggcgctggct gtcacgcaaa attacatcag taattccgca gccagccgtg caatcaatac
    37441 gttagagagc aagacaggaa catcgagctg tttttgcagt aaatcgcgat gacgctggtt
    37501 aaatcccaaa caatccagca tgatgacatc tgcacctttt gccagtaatt ctttcccggc
    37561 atcaatgatt ttttgttccg aatcatgaat ggggttaccc aatgaaaata ctggcggttt
    37621 ctgcaaaatt tgccattttt gcgcctgaac ggtcagcaac tcctcaaccg gtacgataac
    37681 ccccacctga tgatcttcaa caatagagga aaccagcggt ggcaatatcc gcgacggctc
    37741 aagaaagatc gtattacgcg cggtcatact actaatgttt gctgtactca ttaatataat
    37801 gacgtcgtaa ccctgattat cgagcacttc aaccacacct tgcaggtctc gctccacttt
    37861 gcgacgcgaa acatgggcca gatggttgtc atttaataat gtgagaattg tgtcttcgcc
    37921 tgcttctggc gcgtactccg ccatcacttc ttcgcggctt aacttcccca gcaggctatg
    37981 atgggaaata ttatcttcgt caatgtattc tgtcaggagc ggcaaaactt cctgcatagg
    38041 tacaatgccg atagtcagga tcgccaacga cgcactcatt gttcttccac cttacttttc
    38101 tttcgtcgtt aattaccggg caagtgttaa gcaccactgc tgacacttac ttgtgctaca
    38161 aatgacaaaa agcgtagcag cagacctgca ct
//
LOCUS       DAZFRG010000016        37649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFRG010000016 DAZFRG010000000
VERSION     DAZFRG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.016"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            424..1794
                     /gene="aroP"
                     /locus_tag="ACHKUC_000619"
     CDS             424..1794
                     /gene="aroP"
                     /locus_tag="ACHKUC_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO6616927.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTTDTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1837..2691)
                     /gene="ampE"
                     /locus_tag="ACHKUC_000620"
     CDS             complement(1837..2691)
                     /gene="ampE"
                     /locus_tag="ACHKUC_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO6616928.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2688..3239)
                     /gene="ampD"
                     /locus_tag="ACHKUC_000621"
     CDS             complement(2688..3239)
                     /gene="ampD"
                     /locus_tag="ACHKUC_000621"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO6616929.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3327..4220
                     /gene="nadC"
                     /locus_tag="ACHKUC_000622"
     CDS             3327..4220
                     /gene="nadC"
                     /locus_tag="ACHKUC_000622"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO6616930.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4424..4864
                     /gene="ppdD"
                     /locus_tag="ACHKUC_000623"
     CDS             4424..4864
                     /gene="ppdD"
                     /locus_tag="ACHKUC_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO6616931.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4874..6259
                     /gene="gspE"
                     /locus_tag="ACHKUC_000624"
     CDS             4874..6259
                     /gene="gspE"
                     /locus_tag="ACHKUC_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO6616932.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQMLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6249..7451
                     /gene="hofC"
                     /locus_tag="ACHKUC_000625"
     CDS             6249..7451
                     /gene="hofC"
                     /locus_tag="ACHKUC_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO6616933.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQHQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7486..8529)
                     /gene="guaC"
                     /locus_tag="ACHKUC_000626"
     CDS             complement(7486..8529)
                     /gene="guaC"
                     /locus_tag="ACHKUC_000626"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO6616934.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8685..8729)
                     /locus_tag="ACHKUC_000627"
     CDS             complement(8685..8729)
                     /locus_tag="ACHKUC_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6616935.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8754..9374
                     /gene="coaE"
                     /locus_tag="ACHKUC_000628"
     CDS             8754..9374
                     /gene="coaE"
                     /locus_tag="ACHKUC_000628"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO6616936.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQR
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9374..10117
                     /gene="zapD"
                     /locus_tag="ACHKUC_000629"
     CDS             9374..10117
                     /gene="zapD"
                     /locus_tag="ACHKUC_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO6616937.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10127..10324
                     /gene="yacG"
                     /locus_tag="ACHKUC_000630"
     CDS             10127..10324
                     /gene="yacG"
                     /locus_tag="ACHKUC_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO6616938.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10412..10810)
                     /gene="mutT"
                     /locus_tag="ACHKUC_000631"
     CDS             complement(10412..10810)
                     /gene="mutT"
                     /locus_tag="ACHKUC_000631"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO6616939.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLRRVYVE"
     gene            complement(10870..13575)
                     /gene="secA"
                     /locus_tag="ACHKUC_000632"
     CDS             complement(10870..13575)
                     /gene="secA"
                     /locus_tag="ACHKUC_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO6616940.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13637..14149)
                     /gene="secM"
                     /locus_tag="ACHKUC_000633"
     CDS             complement(13637..14149)
                     /gene="secM"
                     /locus_tag="ACHKUC_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO6616941.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14380..15297)
                     /gene="lpxC"
                     /locus_tag="ACHKUC_000634"
     CDS             complement(14380..15297)
                     /gene="lpxC"
                     /locus_tag="ACHKUC_000634"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO6616942.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15398..16549)
                     /gene="ftsZ"
                     /locus_tag="ACHKUC_000635"
     CDS             complement(15398..16549)
                     /gene="ftsZ"
                     /locus_tag="ACHKUC_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6616943.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQAQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16610..17872)
                     /gene="ftsA"
                     /locus_tag="ACHKUC_000636"
     CDS             complement(16610..17872)
                     /gene="ftsA"
                     /locus_tag="ACHKUC_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO6616944.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17869..18699)
                     /gene="ftsQ"
                     /locus_tag="ACHKUC_000637"
     CDS             complement(17869..18699)
                     /gene="ftsQ"
                     /locus_tag="ACHKUC_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO6616945.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPGESTQQQNQAQAEQQ
                     "
     gene            complement(18701..19621)
                     /gene="ddlB"
                     /locus_tag="ACHKUC_000638"
     CDS             complement(18701..19621)
                     /gene="ddlB"
                     /locus_tag="ACHKUC_000638"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6616946.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19614..21089)
                     /gene="murC"
                     /locus_tag="ACHKUC_000639"
     CDS             complement(19614..21089)
                     /gene="murC"
                     /locus_tag="ACHKUC_000639"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO6616947.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21143..22210)
                     /gene="murG"
                     /locus_tag="ACHKUC_000640"
     CDS             complement(21143..22210)
                     /gene="murG"
                     /locus_tag="ACHKUC_000640"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO6616948.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22207..23451)
                     /gene="ftsW"
                     /locus_tag="ACHKUC_000641"
     CDS             complement(22207..23451)
                     /gene="ftsW"
                     /locus_tag="ACHKUC_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO6616949.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23451..24767)
                     /gene="murD"
                     /locus_tag="ACHKUC_000642"
     CDS             complement(23451..24767)
                     /gene="murD"
                     /locus_tag="ACHKUC_000642"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO6616950.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVCLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24770..25852)
                     /gene="mraY"
                     /locus_tag="ACHKUC_000643"
     CDS             complement(24770..25852)
                     /gene="mraY"
                     /locus_tag="ACHKUC_000643"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO6616951.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25846..27204)
                     /gene="murF"
                     /locus_tag="ACHKUC_000644"
     CDS             complement(25846..27204)
                     /gene="murF"
                     /locus_tag="ACHKUC_000644"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO6616952.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27201..28688)
                     /gene="murE"
                     /locus_tag="ACHKUC_000645"
     CDS             complement(27201..28688)
                     /gene="murE"
                     /locus_tag="ACHKUC_000645"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO6616953.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28675..30441)
                     /gene="ftsI"
                     /locus_tag="ACHKUC_000646"
     CDS             complement(28675..30441)
                     /gene="ftsI"
                     /locus_tag="ACHKUC_000646"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO6616954.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30457..30822)
                     /gene="ftsL"
                     /locus_tag="ACHKUC_000647"
     CDS             complement(30457..30822)
                     /gene="ftsL"
                     /locus_tag="ACHKUC_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO6616955.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30819..31760)
                     /gene="rsmH"
                     /locus_tag="ACHKUC_000648"
     CDS             complement(30819..31760)
                     /gene="rsmH"
                     /locus_tag="ACHKUC_000648"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO6616956.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31762..32220)
                     /gene="mraZ"
                     /locus_tag="ACHKUC_000649"
     CDS             complement(31762..32220)
                     /gene="mraZ"
                     /locus_tag="ACHKUC_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO6616957.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32822..33826)
                     /gene="cra"
                     /locus_tag="ACHKUC_000650"
     CDS             complement(32822..33826)
                     /gene="cra"
                     /locus_tag="ACHKUC_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO6616958.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33908..33994)
                     /locus_tag="ACHKUC_000651"
     CDS             complement(33908..33994)
                     /locus_tag="ACHKUC_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO6616959.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34006..34497)
                     /gene="ilvN"
                     /locus_tag="ACHKUC_000652"
     CDS             complement(34006..34497)
                     /gene="ilvN"
                     /locus_tag="ACHKUC_000652"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6616960.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34500..36224)
                     /gene="ilvI"
                     /locus_tag="ACHKUC_000653"
     CDS             complement(34500..36224)
                     /gene="ilvI"
                     /locus_tag="ACHKUC_000653"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO6616961.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSYSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36343..36381
                     /locus_tag="ACHKUC_000654"
                     /pseudo
     CDS             36343..36381
                     /locus_tag="ACHKUC_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36542..37486)
                     /gene="leuO"
                     /locus_tag="ACHKUC_000655"
     CDS             complement(36542..37486)
                     /gene="leuO"
                     /locus_tag="ACHKUC_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO6616962.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9145 a        10466 c         9132 g         8906 t
ORIGIN      
        1 cgttttcact tagctcgcaa tattatattt aaatactcgc gttacaaccc cattgtatta
       61 ttaacatcta tttattctta tatgaccttt tgtaagatta ttgtgctggt ggctaaggca
      121 tggtgttcag caggcagaca aatccgacat atagctagtt aaaacagtat gttatcagac
      181 ttaaagtatc tattaaatgt gatgaaaaac ttacaattgc ttttttttta tccagaaaat
      241 caacacatct acataaagca ggtgcattct cgcgcatata ccattatctt gtgatagttg
      301 aaagtactat catcataatt cagttacata aaatgtaaag aaaccaatac aacaaacgga
      361 attacaaaat tacacacgca ttgctgcgta gttcaaaaaa acaacacaac acgaggtttc
      421 ataatggaag gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt
      481 cagcttatcg cgctgggtgg cgcgataggg acagggctat tcctgggtag cgcctccgta
      541 atacagtccg cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt
      601 ctgatcatgc gtcagctagg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc
      661 cactttgctt ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg
      721 gtactgtacg ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc
      781 tggtatccgg aaatcccaac ctgggtttct gccgccgtgt tctttgtggt gatcaacgcc
      841 atcaacctga caaacgtaaa agtgtttggc gagatggagt tctggtttgc cattatcaaa
      901 gttatcgcgg tggtagcgat gatcatcttc ggcgcctggc tgctattcag cggcaacggc
      961 ggcccgcagg cgagcgttag caacctgtgg gatcagggtg gtttcctgcc acacggcttc
     1021 accggtctgg tgatgatgat ggcgattatc atgttctctt tcggtggtct ggaactggtg
     1081 gggatcaccg cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag
     1141 gttatctacc gcatcctgat tttctatatt ggctcgttag ccgttctgct ctcactaatg
     1201 ccgtggaccc gcgttaccac cgataccagt ccgtttgtgc tgatcttcca cgagttaggc
     1261 gatacctttg tagcgaatgc gctgaatatc gtggtactga ctgctgcgct ctccgtgtac
     1321 aacagctgcg tatattgcaa cagccgtatg ctgttcggtc tggcacaaca agggaacgcg
     1381 ccaaaagcgc tggcgtctgt cgataaacgc ggcgtaccgg ttaacactat tctggtgtct
     1441 gcgctggtta cggcattgtg cgtactgatt aactaccttg ccccagagtc cgctttcgga
     1501 ctgttaatgg cgctggtggt atctgcactg gtcatcaact gggcgatgat cagtctggcg
     1561 catatgaaat tccgtcgtgc caaacaagaa caaggcgtgg taactcgctt ccctgccctg
     1621 ctttatccgc tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg
     1681 ctgatgaccc caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta
     1741 ggtatcggct atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc
     1801 tacgccctca cccgtgcagg gtgagggcaa taatctttac accaacgccc cgtaaatcgt
     1861 cagtagcgca attaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc
     1921 ctttggcgtc tccaccttat cgacatgcgg ttcacgcgcc agagagaact gtgccagacg
     1981 cgttaatacc tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa
     2041 cgctttctca ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca
     2101 atccagtaca tgaagcacgc catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta
     2161 tcgcgccagc cagtattgcc atgcacgcaa aaacgcatac cccatcagcg taacgggtcc
     2221 ccaggttccc cccacaatca gccagaacag cggagcaaga taaaaacgaa agttaatcca
     2281 cagcaatgca ttttgcagct cacgcaaata ctcacgttca tcgcagcctg ccgggacgcc
     2341 gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc
     2401 agctgtcaga taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc
     2461 aatcagcagc cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa
     2521 cagtaaaaaa gtcacgccca tcgcaataat ggtcatgcct aatgtgcgcc cgagagaaaa
     2581 atgtttcacc cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa
     2641 cttaaacagg cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc
     2701 ttgctgacca gcgcacgaaa ccgtgcccaa tcaaatgcag ggccgggatc ggttttccga
     2761 tccggcgcaa tatcacaatg gcccgtcatg ttattagcga tatccggata gcgatcaatc
     2821 agtgcccgcg taaccgccgc aagctgttga tactgcgcat cggtatacgc cagcgtatcg
     2881 gtgccttcca gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga
     2941 gagactcccg catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatcg
     3001 cggcgaatca aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgc
     3061 gcctgcggat caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct
     3121 ggcggcaggc taatattgtg caccaccagc agagtgggtg tttcgtcatc cgggcggcaa
     3181 tcgtaatgtg gtgagggaac gcggcgcgcg ccagccagcc acccctgttc taacaacatg
     3241 caggatctcc ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg
     3301 attcgttagc tatctggagt tttaacatgc cgcctcgccg ctataaccct gacacccgac
     3361 gtgacgagct gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc
     3421 gggaagattt aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg
     3481 aaaactctcg ctctcatgcc acagtgatca cccgcgagaa tggcgtattt tgcggcaaac
     3541 gctgggttga agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg
     3601 tggatgacgg cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatctc
     3661 gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg
     3721 ccagtaaggt acgccactat gtcgaattac tggaaggcac caacacgcag ctgttggata
     3781 cgcgcaaaac cttacccggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg
     3841 gagcgaatca ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg
     3901 cctccggctc agtgcggcag gcggtcgaaa aagcgtcctg gttgcacccg gatgcgccag
     3961 tagaagtcga agtagagaat ctggaagaac ttgatgaagc tctgaaagca ggtgccgata
     4021 tcatcatgct ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg
     4081 gcaaggcgct actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg
     4141 aaacgggcgt ggactttatc tccgttggtg cactaactaa acacgtacag gcactcgacc
     4201 tttcaatgcg ttttcgctaa ttgctaacag atttatccgc tccatcgcgg gcggataaat
     4261 ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga
     4321 aataaaaaac ctcggaagct gctttcccgt ttcgtttttt gcccctcgct tgtgctgatg
     4381 gcgtttgcca aagtagcgcc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg
     4441 ttttacactt atcgaactga tggtggttat tggcatcatt gccattttaa gcgccattgg
     4501 tattcccgct tatcaaaact acctgcgcaa agccgcactc accgacatgc tacaaacctt
     4561 tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa catggtggat taaatacctg
     4621 cgacggtggc agcaatggca ttccctcgcc taccaccacc cgctatgttt cagccatgag
     4681 tgtggcaaag ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatggac taagcgtcgt
     4741 catgacgccg ggttgggata acgcaaacgg cgtcaccggc tggacgcgta actgcaatat
     4801 tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa
     4861 ctaaggagcg gcaatgaata ttccacagct cactgccctg tgtctgcgtt atcagggagt
     4921 cttgctggat gccagcgaag aggtggttca tgttgcggta gtcgatgcac cttcgcatga
     4981 gctactggac gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg
     5041 ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca ttgcccgtag ctgttcagga
     5101 gaagcatcag cccaaagcag agttgctgac tcgaacgtta caatctgcgc tggaacaacg
     5161 cgcgtctgat attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga
     5221 cggcgtattg catcctttac cggacgtttc accggatgcc ggagtcgcat taaccgccag
     5281 attaaaagtg ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt
     5341 cactgtcgaa ctggcaggaa atgccgtctc atttcgtatt gcgaccttag catgtcgggg
     5401 tggtgaaaag gtggtattaa ggttgttaca gcaggtgaac caggcactgg atgttaacac
     5461 gcttggaatg cagccgtcac aactggtgga ctttgctcat gccttgcaac aaccacaggg
     5521 actggtgctg gtaactggcc ctacaggcag cggcaaaacg gtcacgcttt atagtgccct
     5581 gcaaatgctg aataccgctg acattaatat ttgtagcgtc gaagatccgg ttgagatccc
     5641 catagccgga ctaaaccaga cgcaaatcca ttcgcgtgcc ggactcacct ttcagggcgt
     5701 tttgcgtgcg ttattgcgcc aggatcctga cgtcatcatg atcggagaga tccgcgatgg
     5761 cgaaacggca gaaattgcca ttaaagccgc gcaaaccggt cacctggtgt tgtctaccct
     5821 acacactaac tccacctgcg aaacgctggt acgtttacag caaatgggag tcgcccgctg
     5881 gatgctatca tcggcgctta cgctggtaat agcccagcgt ctggtacgca aactttgccc
     5941 gcattgtcgc cggcagcaag gggagcccat ccatattcca gacaatgtat ggccatcgcc
     6001 gctgccccac tggcaggcac ccggttgtgt acattgctac cacggttttt atggtcgcac
     6061 ggccttattt gaagttctgc ccataacacc ggtcatacgt cagcttattt ccgctaatac
     6121 cgacgttgaa tcgctggaaa cgcacgcccg acaggcgggt atgcgaacgc tttttgaaaa
     6181 cggctgcctg gccgtggagc aaggcttaac cacctttgaa gagttaatcc gcgtattggg
     6241 gatgccgcat ggcgagtaag caactctggc gctggcatgg cataaccggc gacggcaatg
     6301 cgcaagatgg gatgctatgg gcagagagcc gtgctttgct gctcatggca ctacagcaac
     6361 agatggttac cccactaagc ctgaagcgaa tcgccatcaa ttctgcgcag tggcgaggag
     6421 ataaaagcgc ggaagtcatt catcaactgg cgacgctact caaagccggg ttaacgcttt
     6481 ctgaagggct ggctctgctg gcggaacagc atcccagtaa gcagtggcaa gcgttgctgc
     6541 aatcactggc gcacgatctt gaacagggcg ttgccttttc caatgcctta ttaccctggt
     6601 cagaggtatt tccgccgctc tatcaggcga tgatccgcac gggtgaactg accggtaagc
     6661 tggatgaatg ctgctttgaa ctggcgcgtc agcaaaaagc ccagcatcag ttgaccgaca
     6721 aagtgaaatc agcgttacgt tatcccatta tcattttagc gatggcaatc atggtggttg
     6781 tggcaatgct gcattttgtt ctgccggagt ttgccgctat ctataagacc tttaacactc
     6841 cgctaccggc gctaacgcag gggatcatga cgctggcaag ttttagtggc gaatggggtt
     6901 ggttgctggt gctattcggc tttctgctgg cgatagccaa taagctgctg atgcgccacc
     6961 caaccaggct tatcgcacgg cagaaattgc tgttacgcat cccgattatg ggttcactga
     7021 tgcggggaca aaaactcacg cagatcttta cgattctgac gctgacacaa agtgcaggca
     7081 tttctttttt acagggcgtt gagagcgtca gagaaacaat gcgctgcccg tactgggtgc
     7141 aacttctgac acaaatccag cacgatatca gtaacggtca ccccatctgg ctggcgctaa
     7201 aaaatgccgg tgagtttagc ccgctctgtt tgcaattagt gagaacagga gaggcatccg
     7261 gctcgctgga tctcatgtta gacaacctcg cccatcatca tcgggataac acaatggcgc
     7321 tggcggataa cctcgcagcc ttactggaac cggcgttgct gatcataacg ggaggaatta
     7381 tcggtacgct ggtggtggca atgtatctgc caattttcca tttaggcgat gcgatgagtg
     7441 gaatgggata acgctggcgt gttgctccac gccagcgttg gtatattaca ggttgttgaa
     7501 gatgcggttt tcttgttcct gcacacggat aaacgtggtg cgcttggtca gttctttcag
     7561 gcgtgaagcc ccaacgtatg tacaagctga acgcaggccg cccaaaatat cacgcgcggt
     7621 attttcaacc gggcctcgca gcggcagctt aacggtttta ccttctgctg cgcgatattc
     7681 cgcaacgccg ccaacgtgac gtttcatcgc agactctgag ctcatgccgt agaacaacat
     7741 aaatttctcg ccgttctcct caacgatgcg accgccgctc tcttcgtggc ccgccagcat
     7801 gccgccaagc atgacgaaat cggcaccgcc gccaaaggct ttcgcgacat cgcccggcgt
     7861 ggtgcagccg ccatcgctga cgatcattcc gcccagaccg tgcgcagcat cggcgcattc
     7921 gatcaccgca gaaagttgcg gataaccgac gcctgttttg acgcgagttg tacaaacaga
     7981 acctgggcca atgccaactt taacgatatc ggcaccggag aggataagct cctcacacat
     8041 ttcaccagtc actacgttgc ctgcacaaat ggttttggtc ggccacgctt cacgcgcttt
     8101 cgcaacgaac tgcacgaagt gttcggaata accattcgcc acgtcaatac aaacgaaatt
     8161 taatgccggg ttcaggtcga gaatctgttt agttttttcg aaatccgcat cagacgtgcc
     8221 ggtagaaacc atcacatgtt tcagcacatc agcggaagaa ttgttgataa acgcttgcca
     8281 ctcttcgaca gaatagtgtt tatgcacagc agtcagaata tcaaaagaag ccagcgcaga
     8341 ggccatagaa aatgtgccta cggtgtccat attggcggcg ataatcggca cgccggacca
     8401 gctctgacct gaatgtttga aggtgaattg acgttccagt tcaacatcgg aacggctttt
     8461 aagagtggag cgtttagggc ggatgagaac gtctttaaaa cctaacttca gatcttcttc
     8521 aatacgcatg tgcggattcc tggggttaat ggctataagg gtaagagcgc aactccagtg
     8581 acgttatcat acgcactaat caatgcggcg caagactgcg aaattgtctt tttttacgct
     8641 acaatcccat aaatttacct ggtaaatagt gagcgaacgt ttcatcaatt tcgctttcgt
     8701 gcgctcattt ttaacgtttt gataatcatg atgaattccc gggaatataa attatgaggt
     8761 atatagttgc cttaacggga ggcattggca gtggcaagag taccgttgcc aatgcgtttg
     8821 ctgatctcgg aattaacgtc attgatgccg atattattgc gcgtcaggtg gttgaaccag
     8881 gtgcacctgc gctacatgcc attgctgatc actttggcgc taacatgatt gctgctgatg
     8941 gaacattgca gcgccgggcc ttgcgcgagc ggatcttcgc caacccggaa gagaaaaact
     9001 ggcttaacgc cctgctgcat ccgctgattc agcaagagac gcaacaccag atccagcgag
     9061 ctacttcccc ctatgtacta tgggttgtgc cattgctggt agaaaactca ctgtataaaa
     9121 aagcgaatcg agtgctggtg gtggatgtca gcccagaaac gcaacttaag cgcaccatgc
     9181 agcgcgacga tgtaactcgc gagcatgtcg aacaaatcct tgctgctcag gcaacgcgcg
     9241 aagctcgcct tgccgtggca gatgacgtca ttgataataa cggcgcaccg gatgctatcg
     9301 catcggatgt tgcccgcctg cacgcacact atttgcagct tgcgtcgcag tttgtctcac
     9361 aggaaaaacc gtaatgcaga cccaggtcct ttttgaacat ccactaaatg aaaaaatgcg
     9421 tacatggctg cgcattgagt ttttgattca gcaactcacc gttaatttac ccatcgttga
     9481 ccacgctggc gcgctgcatt tcttccgtaa tgtcagcgaa ttactggatg ttttcgagcg
     9541 cggcgaagtc cgcactgagc tgttgaaaga acttgaccga cagcaacgta aactccagac
     9601 ctggattggc gtgcccggcg tggaccagag ccgtattgag gcattaattc agcagttaaa
     9661 agcggcgggg agcgtattaa tctccgcgcc gcgtatcggg caatttctac gtgaagatcg
     9721 tttgattgct ctggtgcgtc agcgactgag catcccgggc ggctgttgca gctttgattt
     9781 acctacattg cacatttggc tgcatctacc ccaggcgcag cgcgacagcc aggtagaaac
     9841 ctggattgcc agcctgaacc cgcttaccca ggcacttacc atggtgctgg atttaattcg
     9901 ccagtcggcc cccttccgta aacaaaccag cctgaatggt ttttatcagg ataacggtgg
     9961 cgatgccgac ttgctgcgcc tgaatctgtc gctcgattca cagctttatc cgcaaatttc
    10021 cggtcataag agccgttttg ccattcgttt tatgccgctg gacagtgaaa acggacaggt
    10081 accagaacgt ctggatttcg aactggcctg ttgctaagga gtaaaaatgt cagaaactat
    10141 tacggtgaat tgcccaacct gcgggaaaac ggtggtgtgg ggtgaaatca gcccgtttcg
    10201 gccattttgc tccaaacgtt gtcagctgat cgacctcgga gaatgggctg ctgaagaaaa
    10261 acgaatcccc agcagtggcg atctttccga aagcgatgac tggagcgaag agccaaagca
    10321 gtgacattct atctgatgcg acgctggcct gataaaacgc gccagcgtcg catcaggcat
    10381 catatgcaaa tatcggatgc agcgtgacgc cttattcgac atacacacgc ctaagtttcg
    10441 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    10501 actcaccggg ttgcccttct ttaccccacg gctccccttc ccagctttcg accagccaga
    10561 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    10621 gttgtggggt aatcccgact tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    10681 cgcccatttc aactttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    10741 cgcgacgcgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    10801 gctttttcat tgacttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    10861 tgttagcttt tattgcaggc ggccatggca ctgcttatat tttttaccag aaccacacgg
    10921 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    10981 agcagagtcg tcatcctgat ggctaagctg ctgcatttgc gctaaacgct cggcttccat
    11041 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    11101 cgtgctgata acttcatatt ttaacgactc cagcatcgct gcaaacatgg agaacgattc
    11161 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    11221 cagatagtcc atcgcagcca ggtgctcttt ccacagagag tcaagcgttt gcagcatgac
    11281 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    11341 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    11401 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    11461 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtcgc
    11521 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    11581 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    11641 cagttgctta cgaatgtcga agttacggct ttcaacttta cgctgggcgt tggcaatcgc
    11701 tttggtcacc cacgggtgct caatggcttc gcctggcttc atacccagtt tacgcatcat
    11761 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    11821 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcagctggt tatcgatacg
    11881 acgggattcg tgacgttcag taccgatgat atgcaggcca cctgctgcca gtaccgcatc
    11941 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcagtcg gattttccag
    12001 cgcggcaact tctgcctgcc agctaccacc gagcacaata tcggtaccac gacccgccat
    12061 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    12121 ggcatggaat ttggcgttca ggacgttgtg cttaataccg gctttggtca gttcatttga
    12181 caccagctcc gatttttcga tggagattgt acccaccagc accggctggc ctttcgcagt
    12241 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    12301 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagctt
    12361 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    12421 ttcatacaga cggaagtagt tctggaaggt gatcgacgcc agcgtctggt tttcgttctg
    12481 gatctgtaca ccttctttcg cttccacagc ctgatgcagg ccatcggacc aacgacgtcc
    12541 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    12601 gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    12661 catgatgttg gccggagagt acagagactc accttcatcc atgatgcctt ctttaaccag
    12721 cagttcttca atcagaacca gaccacgttc ggtcaggttc acctggcgag atttttcatc
    12781 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    12841 gtgtggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    12901 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    12961 atagtgcagt ttacgctgta cacgttcttc aggactgaac gccatgttgt cgcgcaggta
    13021 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcacgctt
    13081 tgccggtgct ggcatgcccg gcaggttgat accaactgtc aggccaagaa attcaaacag
    13141 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    13201 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    13261 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    13321 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    13381 cgggatcaga ttttccagca cttcgccttt ttccagacgt gcacgaaact ctgcggtttt
    13441 ccctttcagt tcttcgtcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    13501 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    13561 taatttgatt agcataataa aatctcaaac gccccgcgtt gcggagtcaa taaaatgaag
    13621 taacggttta ttgttgttag ctgaggcgtt gagggccagc acggatgcct tgcgcctggc
    13681 ttatccagac gggcgtgctg aattttgctt gcggggtaaa atgcgcataa tcaatgcgat
    13741 aacccttttc agacggcgtg ccttcctggg tcaacagcgc gctgagcgta tccagtaatg
    13801 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagcgtt tgcggtgcca
    13861 ttgcgaagga aagatgacgg attaccgtgc gaatggcatg ttgatgccag taatcaacgg
    13921 aatagttcga attcgggcgg cgcgtgttgg cttccagcaa ggccaattga ccaaagttaa
    13981 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    14041 cgttgctgag tgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    14101 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    14161 cccgatcgta ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    14221 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    14281 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    14341 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cggctgaagg
    14401 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    14461 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    14521 ggtaaatgca ccgataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    14581 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    14641 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    14701 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaatgcatc
    14761 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    14821 gatggtgaaa tccagagaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    14881 ttcgacacga acagtttctt taatgcgaac aaatttcttg gcgcagttca actcgtcgat
    14941 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    15001 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    15061 gtgctctacg gttgaaatcc gcacatcatg ctcgttgacc agacacgtac agagcatggt
    15121 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    15181 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    15241 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    15301 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    15361 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgcagga
    15421 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacga
    15481 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccgtgctgc tggtagcgat
    15541 ccatcactgg ctgctgagcc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    15601 tgccgatacc tgtcgcaaca acagttacgc gcagctcgtc attcatatcc gggtcaagag
    15661 aagtaccgat aaccacggtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    15721 tttcgaactc atccagacgc aggtcgaagc ccgctgtgat gtttaccagt acgccgcgtg
    15781 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    15841 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    15901 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    15961 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    16021 agataccacg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    16081 catgcttgga cagttcagtg atcccctgct ccgcgaatgc catacgcttc ttgccttcaa
    16141 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    16201 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    16261 tgtctgcacc ttccagcgcc gcacgcagtg catcgcgatc ctcatcagcc gcattgcggc
    16321 caacttctgg attagcgcca gctcccagtc ctttggtgat accgctaccg atttgaatcg
    16381 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    16441 caccttcaat gcgctcacgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    16501 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    16561 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    16621 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    16681 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    16741 tcgaataata cggctcctga gcataatccg ttaaaccggt aatattcagc ggcgcgccga
    16801 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    16861 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    16921 tttcctgcaa ctgcaatatc tcttcgttga ccagattgag cagctcggta tagcgcggct
    16981 cgatcacctc tgccagggtc tgacgttgta gactccgcgg cggacgaccg cctacgctcg
    17041 gcacttccac gctctcatct tttccaacga tggagcccag cgcacaaccg tggcgaactt
    17101 taatcgcttc ggcgtcactt ggcggtgtgc caaaggcgta agcgatatca ctggtcacga
    17161 cattgccagc ataaggaatt accttcgtgt ggcgcaatgc cccaccggta taaacggcga
    17221 tatccattgt accaccaccg atatcaacga cgcagacacc cagttcacgt tcatcttccg
    17281 tcaataccga ataacttgat gccagaccgg caaatatcag ttggtcaact ttcagcccac
    17341 aacgttcaac cgctttgacg atgtttttcg ccatatcatt gtgacatgtg atcaggtgca
    17401 cttttgcctg catccgcacg cccgaaagcc ctaccggatt cttgatccct tcctgatagt
    17461 caatcgcata ctcttgcggg atcacatgca acacacgatg ctcatcgcgc acacgcaccg
    17521 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    17581 gcaccatacc aatttcattc tggcagctga tgtgcttacc tgaaagcgcc agatataccg
    17641 aagatatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    17701 ccacggattc gaggtcgttc accccgcctt tatccatacc acgcgacggg cagctgccca
    17761 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    17821 cggtaccaat ctccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    17881 tgcctgtgcc tgattttgtt gctgagtaga ttcccctggc ggcaagggcg cccagcctac
    17941 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    18001 ctgctgctgt aaaaccggat aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc
    18061 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gtcgcgcggt
    18121 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    18181 gccctgcaac acttcattgg cgctgccttc cgggccatac agcattggaa gcacctgctt
    18241 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tatgttgatc
    18301 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    18361 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    18421 gttgacatcc tgggtcataa aggtacctgg ctcacccaat gccaggatcg actgccggat
    18481 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    18541 ttgcgcatct tccatccagc ccaacacgac ccagccgctc accaacactg tcgttaaaac
    18601 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    18661 ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    18721 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccagac
    18781 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    18841 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gcttttagca
    18901 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    18961 tctcatcaga gagatacttc gcctcataat catagaaggt tccggccggt tgaatacgta
    19021 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    19081 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    19141 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    19201 ccggcaaacc cagagaagaa atttctgcta actgcttatc gttaaggcct ttttcaaact
    19261 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccctgc cacagaagtt
    19321 tgctgcgtag tttatccatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    19381 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    19441 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    19501 cgacaggata cgcgtcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    19561 tcagagaaac ttcccgctca gcggaggtcc cgcccaacag gaccgcgatt ttatcagtca
    19621 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgagcaatt
    19681 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    19741 agcatctcgg ctacctgcgc cgggtccggc accagaatgg gatcaatttt cccacgtcca
    19801 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc accagccgga
    19861 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    19921 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    19981 cagcctgcgc gcgccgcttt aatagtggcg tccacttccg tcgggtggtg gccgtagtca
    20041 tcgaccagca ttgccgtacc gctttttcca ttcactggct ctagcgggaa ttcaccgagg
    20101 aaatcgaaac ggcgccccgt cccctggaag ctttccagcg cacgcaaaat agcctcgtcg
    20161 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacga
    20221 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc
    20281 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    20341 taagtcgtgg tctgacgccc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    20401 cacatcaccg cacgaccgta aaacggcagg ttatgcagaa aattaataaa agtctgtttt
    20461 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    20521 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    20581 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagtcc gccgttaacg
    20641 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt ggttgtcgtt
    20701 ttgccgtgtg ttccggcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    20761 tcagcacgac ggatcaccgg aatacgcgct tcatgagctg caacaatttc cgggttatcg
    20821 gcagaaatcg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    20881 ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg tgaccggatt tggcgctaaa
    20941 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    21001 gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    21061 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    21121 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    21181 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    21241 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt gactgctcga
    21301 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tgacggtctt
    21361 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcaccgtta
    21421 acgcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    21481 taaattccgt cactttatgc tgcggttgtc ccgcttcggc atacgcctgt tcaacggatt
    21541 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    21601 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    21661 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    21721 tacgcaccgg gttacccacc acttccgcat tagggaaagc acctggaaac gcctgcatca
    21781 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    21841 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    21901 cgcccatacc gagcaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    21961 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcagac
    22021 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    22081 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg
    22141 ccagccccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgcttccctt
    22201 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    22261 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    22321 gatcagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    22381 cagcgcctgg aaactaaacc agataccaat agaacaggcg agaaaaccgg aaaaacggtg
    22441 gtcaatttct aatgctttgc ggccaatcga catcgcgcga aaagcgacga agaataccat
    22501 taaaagtgcc agcaccacac cgacataccc cagttcttcg ccgataatgg cgaaaataaa
    22561 gtcagtgtgc gcttccggca gatactccag tttttgtacc gagttaccta aaccttgtcc
    22621 ccacagttca ccgcgaccaa acgccatcaa cgattgcgtt aactgatagc cgctgccaaa
    22681 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    22741 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    22801 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    22861 gtctggctgt gccagcagta acactgccaa caccagaatt acgcccatcg gtttcaggaa
    22921 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    22981 aaacagcgac agttttgtca gctccgcagg ctgaatacgc agcaaaccga gatcgatcca
    23041 acgcgatgcc cctttaaccg agctacccac taccagaacg atcatcagca ggatgataga
    23101 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    23161 gatcgccaga atgaatgcca ggatcagata gacgccatca cgcttcgcga agaagaacgg
    23221 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    23281 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    23341 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccagaat
    23401 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    23461 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ttgatcaagg
    23521 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgt agcaagcaag
    23581 cgcatcgcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    23641 tgcgcgccgt cacgaccgaa acaatacaga catacgttat cgccattcag gtaacgcgcc
    23701 agtgggctaa agtccgccga tttaccatcg ccccccagca acaaatgcag cgtgccgtct
    23761 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    23821 acccagcgca caccgttatg ctccagcaca acttcaaagc gatgcggcag accagtgaat
    23881 gtggttaacg ctttcaggct gctggcacgc ggtaaccctg cggcatctgc cagcgccagc
    23941 gccgccagcg cattggtgta gttatgctgc ccggaaagtt tcatttcttt cacgttcagc
    24001 actttctcgc ctttcacccg cagccaggtt tcaccctgct gatgattcag gtgatagtcg
    24061 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    24121 gcatcatcag cattaaccac gcaaactttt gcgttttcgt aaatgcgcag ttttgctgca
    24181 cgatactgtt gtaaaccaaa cggatagcga tccatatgat cttcagtcac gttcagaatg
    24241 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    24301 acgtacagtt cgcactcatc atccaacagc atcaacgcag gcaggccaat attgccaccc
    24361 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    24421 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcacgaca gaacagctcg
    24481 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    24541 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagaccg
    24601 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    24661 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcaaga aagcccggtg
    24721 aggcccaggc cgataatgac gacattttta ccctggtaat cagccatgat taacgtacct
    24781 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    24841 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    24901 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacggaaagc gtttctacca
    24961 cgaacacgcc ccccataatc accagcagga attcctgacg cagcagtacg gcgataatac
    25021 ctaacgcacc acctaacgcc agcgaaccta catcgcccat aaagacctgc gccggatagg
    25081 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca atgaccagtt
    25141 ccccggcgtg tcgtagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    25201 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    25261 cgagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacaaag taagccagca
    25321 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaacggaacc accagctgcg
    25381 ttgcgggcgt gtctttgccg gcaaggtaca gcgcgaaggc aacacccagt gcaatgaccg
    25441 acatccagaa atacttccaa cgggcaatca ggcccttggt gtctttacgc accactttgc
    25501 gataatcatc aacaaagcca ataacaccat aacctaccag caccaccagt acgcaccaga
    25561 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgcc gtcaggatca
    25621 ttatcccgcc catggtcggt gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    25681 gcaccacctg accaaaggaa agtttttgca aatgagcaat catacgcggg cccatccaca
    25741 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    25801 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    25861 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    25921 cgtaattacc tgttgctcag caatcagtga tttaagacgc gcgataagcg cagttttatc
    25981 ggaaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccacact
    26041 taacacgcag tcaataccag cagcttttgc cgcttcgccc acctgtacat ggcaggcttc
    26101 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    26161 agccagaacc tgaacagcgg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    26221 cagcaactgg ttttctgcca gttttatggg gaacagacgg cctggaacag ctttcagatt
    26281 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    26341 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    26401 tgtaggggtt tgcagggtaa attccgtacc gtgcgaggtt acatggatat tggtggcggt
    26461 gaaatcgctg ttggcggcat taggtgagaa acgccacact ttgcgtgagc caattacgct
    26521 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    26581 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccaa
    26641 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    26701 ggcaatttca ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    26761 taagcgcaac agcgtcatcg gtacaccgat gtcgttattg agattgcctg ccgtataaag
    26821 tgtgttgccg cactggctta aaattgccgc cgtcatctct ttaacggagg ttttgccgga
    26881 ggaacctgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    26941 accaaacgcc agacgcgtat ccttgacgat taactgcggc agatcaatat ccagcggacg
    27001 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat catgagcatc
    27061 gaaacgttcg cctttcaggg caacaaacag gcagcccggc gtcagttttc gcgtgtcagt
    27121 ggttacagca tcaagggtaa tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    27181 ttggctaagg gttacgctaa tcatgcaatc acccccagca gacgcgccac cgtgacgcga
    27241 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    27301 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    27361 ccttccatca ctttggcata tccggcatct aacattcccg ccagaatatc gttgatgatg
    27421 gcacgaggct cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    27481 tcagcaattg cgcccatcag tggacgtttg cctttatcac gatcgccacc gcagccaaag
    27541 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    27601 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggtgcagt gaacacttcc
    27661 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcaaatcagc cagcggatag
    27721 ccgagtgcca gcagtgtcgc cagcgccagc agcaggttgc tgacgttgaa tgcgcccatc
    27781 aggcgacttt caatttcccc atcgccccaa ctcgagctaa agcgaatggt cgcaccgctg
    27841 tcgtgatagt tcacgtcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    27901 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    27961 tcgtcggcgt taataatcgc ctgaccgcaa tgatgctcag aataaagcag ccattttgca
    28021 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    28081 accgacgccg caaatttcaa tgccgccaca cggtgttgta ccagcccgtg ggatgaaact
    28141 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatcg
    28201 actgccgaac cggtggtatt ttctgtcggg atcactttcc ccagcaggcc gttaccaacg
    28261 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgtgccag cagctgggtc
    28321 gtcgtggttt tgccgttggt gcccgttacg cctacaagac gtaaattgtc agagggttca
    28381 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    28441 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    28501 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    28561 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    28621 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    28681 tctgccacct gtaccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    28741 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    28801 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    28861 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    28921 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    28981 tttaatcgcc gccttcacgc cgccgccgcc cggtagcgcc acgctttcca tcatatgcac
    29041 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    29101 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    29161 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    29221 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    29281 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgctaacgc
    29341 cagtttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    29401 gtagcgcgcc acgtctttga tttcgtgacc gttaattcga taaggaacgg tattgagtac
    29461 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    29521 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcttctt tcggcgtgcc
    29581 gctcagatta tttgggttgt atgacgggct gttagccatc gccagcactt caccggtgtt
    29641 gacatccacc agcacggcgc taccagattc agccttgtta aaggccactg cgttgttcag
    29701 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgtcaggt tgtgcgctgc
    29761 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    29821 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcgacac cctcaatccc
    29881 ttggctatcg acgttagtaa aaccgatgag gtgagcagtc acttcgccgg atggataata
    29941 acggcgagat tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    30001 catgtcaggg tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    30061 gcgggctgag agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcgatc
    30121 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgctttaac
    30181 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    30241 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    30301 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    30361 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    30421 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    30481 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gctatccttt ccacccggct
    30541 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    30601 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    30661 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    30721 tcgcaaaaga tcgtcaccga taacgccagg caaggcatgg cgctcgtggc ttcccatcga
    30781 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    30841 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatc
    30901 aacttaccta gtgctcgcaa ctgacggccc cccagttttt tgagctgctc ttctgtcatc
    30961 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaatg
    31021 cggtcttcaa gtgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacattg
    31081 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    31141 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcggcagcc
    31201 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    31261 gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg ttttcaatac ccaggcgata
    31321 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    31381 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gctcagcatc atcaagttgc
    31441 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccgataag atcgcgctcg
    31501 gcaacatatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    31561 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    31621 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    31681 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    31741 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    31801 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    31861 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    31921 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    31981 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctcg acagacgcga
    32041 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    32101 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    32161 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    32221 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    32281 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    32341 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    32401 aatagctgta agaaaaaatc cctcatcgtc atcaccgttt taaaatgact cactattttc
    32461 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttctct
    32521 ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc agcaggtttg cgcaaaaaca
    32581 aagaaaagtg tttgttaaat cggcaattca tctctgcatg taacaaaata atgcaaaaac
    32641 gcgctgtcaa tatcatttaa ttattgtgaa tttcagcaag tgagttaatt aacgggcggg
    32701 tttaaaagca tttatcaata atagccagga atagtcttat ttactttaag gatattgatg
    32761 tccattcccg tactcaacgc gccagagaga aattcacctg gcgcgtactt ttgttcgcgg
    32821 cttagctacg gctgagcacg ccgcggcgat agagattacg tttaatgcgc gttaaaccag
    32881 gttttggctt acgcggttcg tccaggcttg ccaggacaat ctccagcaca cgctctgcga
    32941 catcgcggtg acgttgagcc actgccagca ccgggcactg taagaagtcg agcagttcgt
    33001 tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt gccgtcacga cgtagcgtga
    33061 catccatcac cccttgcaac aacgcaaacg acgttgtgaa cagtgcctgc ggcatcggat
    33121 gcgtttccag ccatttttcg aataactggg cagctgcctc ccgctcatag ctgttggcat
    33181 aaaggaaatg cacttcgcga ggatcatctt tccaggcagt acggaaacct tgttcacgca
    33241 ggaagctgac agaaagctcc ggcagcgcgc caagataaag taccgtctcg gcgggaaact
    33301 tacgtaactc ttccgccagc atttcggcat catcctgatc ggcaccaacc acgctggtga
    33361 agtgttcacg atcgagggcg cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc
    33421 gttgataaaa aggatgctca ggtggcaacg acgtcgaaac aataatggca tcaacctgac
    33481 gctgtaaaag gtgctcaatg cagcgcattt cgttgtcggg ctgatcttcc gagcaggcaa
    33541 tcagcagttg ataaccccgt tgccgcgcct ggcgttcaag atagttagcg atgcgggtat
    33601 agctggtgtt ctccagatcg gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa
    33661 gcccagctgc cacggcgttc gggtggtaat tgtgctcacg caccacagcc atgacttttt
    33721 caacggtttt gtcgctcaca cggtattgct tcgctttgcc gttaataaca tagcttgcag
    33781 tggtccgcga cactcccgcc agccgagcga tttcatccag tttcacaatt gccccttgcg
    33841 taaaatgtaa aaaccatagc cattgtactg gtatgggtta aattcattaa catctaagcg
    33901 cagaaaatta actgcggcaa ctgcttttat tccaggttcc cactgatttc gcaaaaaaag
    33961 cccaacgtga catgttgggc tgcaaattgc gcattgagat cattctcaac gcattatttt
    34021 atcgccgcgt gaaagtccga ccacaccaga gcgagcaact tccacaattt tcgccacatc
    34081 gcgaatcgat gctaaaaatg catcaagctt atcgctggtg cctgctaatt gaacggtata
    34141 aagcgagggt gtgacatcga taatttgccc acggaatatt tccgtattac gtttcacttc
    34201 gtcacgcccg taaccgctgg cctgaatttt caccagcatg atttcccgct caacgtgcgc
    34261 gccctgcccc aactcgctca cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat
    34321 ttgctcaagc actttttcat cgcccacggt ctggatggtc atacgcgata atgtcggatc
    34381 gtcggttggc gcaacggtca ggctttcaat gttgtagcca cgctgggaaa aaaggccaat
    34441 cacgcgggat aacgcgcctg attcattttc gagtaagact gataatatcc ggcgcataat
    34501 caggttctct ccgttttgct taaccacatt tcatccattc cgcccccgcg aatctgcatc
    34561 gggtagacgt gctcactgcc atcgacggta acatcaacaa acaccaagcg attattgcgc
    34621 acctgttcca gcgcctcgct aagtttgctt tccagttctt gcggatgaga gatctggatc
    34681 ccgacgtgac cataggcttc cgccagacgg acgaagtctg gtaaggactg catataagat
    34741 tgtgaatgac ggccggaata gatcatgtcc tgccactgct tcaccatccc cagatagcgg
    34801 ttattgagat tcaccaccag tacgggcaat tcatattgca acgcggtaga cagttcctgg
    34861 atgttcatct gaatactgcc gtcaccagtg acgcaaacca cggtttcttc tggcaacgcc
    34921 attttgacgc ccagtgccgc aggtaaacca aaacccatcg tgccgaggcc gccagagttg
    34981 atccagcgac gcggtttgtc gaatggataa taaagcgcag caaacatctg gtgctgcccg
    35041 acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc aaagagtctc gatcaccgcc
    35101 tgcggtttaa tcttttcact gtaagagtca tatttcaggc actgacgagc gcgccactgt
    35161 tcaatctgct gccaccagtc gcggatctca tccagcggtt gatggacgga ttcttgcgac
    35221 aggagttcaa gcatttgttc gaggacctgg cgagcatccc ccacaatcgg gatatcagca
    35281 gtcacggttt tagaaatgga agtaggatca atatcgatat gcaaaacagt ggcatttggg
    35341 cagtactttg ccagattgtt cgtcgttcgg tcatcaaaac gtaccccgac ggcgaaaatc
    35401 acatccgcgt tatgcatcgt catattggct tcgtaggtac cgtgcattcc caacatgccc
    35461 agtgcctggc gatgcgttgc cggaaacgcc cccagcccca tcaaagagga aacaacgggc
    35521 agattcaacg cctccaccgc ttctttcaac tgcagatggc agcccgccgt gattgcccca
    35581 ccgcctacgt agacaaccgg tttttttgcc gccaccagcg tttgcagagc gcgcttaatt
    35641 tgccctttat gcccggaggt cgtgggattg taagaacgca tactgaccga ctccggccag
    35701 acatagggta atttgttcgc cggatttaga atatctttcg gtaaatcaac gaccacgggt
    35761 ccaggacgac cacttgccgc tagccagaaa gcctttttca gcacctgcgg aatgtcttcc
    35821 gtttgcttaa ccagaaaact gtgtttaacc accgggcgag aaatccccac catgtcgcac
    35881 tcctgaaagg catcataacc tatcaacgaa gtcgctacct gcccggaaag gacaactaat
    35941 ggaatggaat ccatataagc ggtggcaatg ccagtaattg cattggtcgc ccctggaccc
    36001 gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc
    36061 accgccgcct gctcatggcg aactaaaaca tgatcgatac cacccacggt atgcaatgca
    36121 tcgtaaatat caaggaccgc gcctccagga taaccgaata cttgtttaac gccctgatcg
    36181 ataagcgatc ggacgaccat ctcggctcca gacaacatct ccatggcctg cctcactgtt
    36241 tgacggaaaa aatgtgtaaa acacattaac cgtccgatct tgcttaagca attggcagac
    36301 agagacataa acacggggta ataagaataa acaggaggaa aggtgaaaag aaacgataaa
    36361 atcctccatt acagaggatg aaattgataa taaatcggct gaatcctaca acttacagca
    36421 atttctctat ttttttaatt aatcctaaaa tagagagcac aaacattcag aataaaattc
    36481 tacattcaat aaaaaatgca gaataaacca gacattgatg tctgacctat tctgcattca
    36541 gttagcgttt gcaaattgag actaattgct cttccatcca ctgatggcct ttatcacgcc
    36601 cggcagcttc atgccaggag agataacagg ttctgctgtt ttgttttaac ggcagcggta
    36661 atacctgtaa ctctaaggat tcagcgaact cttcagccaa ccaacgcggc gcaatagcga
    36721 ccagatgcgt ttgcgacacc acgctaagta cgctcatcat tgccatgccc tgatacgcga
    36781 tactggcttg cttatctacc gtgtcatacc aggtttgact aaatgatgcg aaacgatcga
    36841 gcgaaaccgc agcatgttgt tcgttataaa catcatgctt cagtaacggg cctttaattg
    36901 ttggatgatt tttgctggct accaacacca tttcatcttt aaataatggc acactggtaa
    36961 attcagggcg atggaagtct tcataactaa tcacaaactc cgtttcctga taacgcagct
    37021 gatgttcagt gttctgattt aatgaagact tgaacataac atgtatattt ggcgcaatct
    37081 gctcaatgtg attataaatc tgcgaggtca gaatgctgtc taacgggctg caaacacaaa
    37141 gatgaaatac acgttcactg ctcgcgggtt caaaacctga accaggcaat tcattttgta
    37201 ctagttgcaa tgcctgacga actgagccaa aaagttgaaa tgcgcgagca gtcggttgaa
    37261 taccacggcc ataacgaaca aaaagctcgt cattaaacat caccttcagg cgtgcaacag
    37321 cgttactgac cgcaggttgc gacattccca gagcatgagc ggcacgggtg atgttttgct
    37381 cctgcatcac ggcatcgaaa acggttaata agttgagatc gaccatgcgt agctgtggct
    37441 tgcttaactc cgccgtctct ggatgatctg tttgtacctc tggcatactt aactccactg
    37501 tcacacttaa ctccctttcc cttattggaa tgcagagaaa taatcctcga gaatatgatt
    37561 tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa ttaggaaaac ataaagatac
    37621 gggttctgat aaaaacagaa tccactgcc
//
LOCUS       DAZFRG010000017        35610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFRG010000017 DAZFRG010000000
VERSION     DAZFRG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.017"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            161..925
                     /gene="yfeN"
                     /locus_tag="ACHKUC_000656"
     CDS             161..925
                     /gene="yfeN"
                     /locus_tag="ACHKUC_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO6616963.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(964..1890)
                     /gene="yfeR"
                     /locus_tag="ACHKUC_000657"
     CDS             complement(964..1890)
                     /gene="yfeR"
                     /locus_tag="ACHKUC_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6616964.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1980..2978
                     /gene="yfeH"
                     /locus_tag="ACHKUC_000658"
     CDS             1980..2978
                     /gene="yfeH"
                     /locus_tag="ACHKUC_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO6616965.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2975..3193)
                     /gene="ypeB"
                     /locus_tag="ACHKUC_000659"
     CDS             complement(2975..3193)
                     /gene="ypeB"
                     /locus_tag="ACHKUC_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO6616966.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(3195..5210)
                     /gene="ligA"
                     /locus_tag="ACHKUC_000660"
     CDS             complement(3195..5210)
                     /gene="ligA"
                     /locus_tag="ACHKUC_000660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO6616967.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTFCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5281..6279)
                     /gene="zipA"
                     /locus_tag="ACHKUC_000661"
     CDS             complement(5281..6279)
                     /gene="zipA"
                     /locus_tag="ACHKUC_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO6616968.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6509..7270
                     /gene="cysZ"
                     /locus_tag="ACHKUC_000662"
     CDS             6509..7270
                     /gene="cysZ"
                     /locus_tag="ACHKUC_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO6616969.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7455..8426
                     /gene="cysK"
                     /locus_tag="ACHKUC_000663"
     CDS             7455..8426
                     /gene="cysK"
                     /locus_tag="ACHKUC_000663"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO6616970.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8810..9067
                     /gene="ptsH"
                     /locus_tag="ACHKUC_000664"
     CDS             8810..9067
                     /gene="ptsH"
                     /locus_tag="ACHKUC_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO6616971.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9112..10839
                     /gene="ptsI"
                     /locus_tag="ACHKUC_000665"
     CDS             9112..10839
                     /gene="ptsI"
                     /locus_tag="ACHKUC_000665"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO6616972.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10880..11389
                     /gene="crr"
                     /locus_tag="ACHKUC_000666"
     CDS             10880..11389
                     /gene="crr"
                     /locus_tag="ACHKUC_000666"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO6616973.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11432..12283)
                     /gene="pdxK"
                     /locus_tag="ACHKUC_000667"
                     /gene_synonym="thiJ"
     CDS             complement(11432..12283)
                     /gene="pdxK"
                     /locus_tag="ACHKUC_000667"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO6616974.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12388..12756
                     /gene="yfeK"
                     /locus_tag="ACHKUC_000668"
     CDS             12388..12756
                     /gene="yfeK"
                     /locus_tag="ACHKUC_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO6616975.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12759..13670)
                     /gene="cysM"
                     /locus_tag="ACHKUC_000669"
     CDS             complement(12759..13670)
                     /gene="cysM"
                     /locus_tag="ACHKUC_000669"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO6616976.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANCGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13805..14902)
                     /gene="cysA"
                     /locus_tag="ACHKUC_000670"
     CDS             complement(13805..14902)
                     /gene="cysA"
                     /locus_tag="ACHKUC_000670"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO6616977.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14892..15767)
                     /gene="cysW"
                     /locus_tag="ACHKUC_000671"
     CDS             complement(14892..15767)
                     /gene="cysW"
                     /locus_tag="ACHKUC_000671"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO6616978.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15767..16600)
                     /gene="cysT"
                     /locus_tag="ACHKUC_000672"
     CDS             complement(15767..16600)
                     /gene="cysT"
                     /locus_tag="ACHKUC_000672"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO6616979.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16600..17616)
                     /gene="cysP"
                     /locus_tag="ACHKUC_000673"
     CDS             complement(16600..17616)
                     /gene="cysP"
                     /locus_tag="ACHKUC_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO6616980.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17774..18565)
                     /gene="ucpA"
                     /locus_tag="ACHKUC_000674"
     CDS             complement(17774..18565)
                     /gene="ucpA"
                     /locus_tag="ACHKUC_000674"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO6616981.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18845..19741
                     /gene="murQ"
                     /locus_tag="ACHKUC_000675"
     CDS             18845..19741
                     /gene="murQ"
                     /locus_tag="ACHKUC_000675"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO6616982.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTTIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            19745..21169
                     /gene="murP"
                     /locus_tag="ACHKUC_000676"
     CDS             19745..21169
                     /gene="murP"
                     /locus_tag="ACHKUC_000676"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO6616983.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            21172..21258
                     /locus_tag="ACHKUC_000677"
                     /pseudo
     CDS             21172..21258
                     /locus_tag="ACHKUC_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21347..22246)
                     /gene="yfeX"
                     /locus_tag="ACHKUC_000678"
     CDS             complement(21347..22246)
                     /gene="yfeX"
                     /locus_tag="ACHKUC_000678"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO6616984.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22342..22917)
                     /gene="yfeY"
                     /locus_tag="ACHKUC_000679"
     CDS             complement(22342..22917)
                     /gene="yfeY"
                     /locus_tag="ACHKUC_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO6616985.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22978..23427)
                     /gene="yfeZ"
                     /locus_tag="ACHKUC_000680"
     CDS             complement(22978..23427)
                     /gene="yfeZ"
                     /locus_tag="ACHKUC_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO6616986.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRALFPRIWHVLYFLLLLAQVVL
                     LCWQPWLRLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23414..23839)
                     /gene="ypeA"
                     /locus_tag="ACHKUC_000681"
     CDS             complement(23414..23839)
                     /gene="ypeA"
                     /locus_tag="ACHKUC_000681"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO6616987.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24053..24922
                     /gene="amiA"
                     /locus_tag="ACHKUC_000682"
     CDS             24053..24922
                     /gene="amiA"
                     /locus_tag="ACHKUC_000682"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO6616988.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24926..25825
                     /gene="hemF"
                     /locus_tag="ACHKUC_000683"
     CDS             24926..25825
                     /gene="hemF"
                     /locus_tag="ACHKUC_000683"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO6616989.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(25831..26883)
                     /gene="eutR"
                     /locus_tag="ACHKUC_000684"
     CDS             complement(25831..26883)
                     /gene="eutR"
                     /locus_tag="ACHKUC_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO6616990.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26929..27429)
                     /gene="eutK"
                     /locus_tag="ACHKUC_000685"
     CDS             complement(26929..27429)
                     /gene="eutK"
                     /locus_tag="ACHKUC_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO6616991.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27442..28101)
                     /gene="eutL"
                     /locus_tag="ACHKUC_000686"
     CDS             complement(27442..28101)
                     /gene="eutL"
                     /locus_tag="ACHKUC_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO6616992.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28111..28998)
                     /gene="eutC"
                     /locus_tag="ACHKUC_000687"
     CDS             complement(28111..28998)
                     /gene="eutC"
                     /locus_tag="ACHKUC_000687"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO6616993.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(29019..30380)
                     /gene="eutB"
                     /locus_tag="ACHKUC_000688"
     CDS             complement(29019..30380)
                     /gene="eutB"
                     /locus_tag="ACHKUC_000688"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO6616994.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30392..31795)
                     /gene="eutA"
                     /locus_tag="ACHKUC_000689"
     CDS             complement(30392..31795)
                     /gene="eutA"
                     /locus_tag="ACHKUC_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO6616995.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGGQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPTDP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31792..32979)
                     /gene="eutH"
                     /locus_tag="ACHKUC_000690"
     CDS             complement(31792..32979)
                     /gene="eutH"
                     /locus_tag="ACHKUC_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO6616996.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGVLAGIVTIPIGCIAGG
                     LVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPEKMINGFQIFAKFL
                     VALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEVIGSISCVLLGAYP
                     MVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMKQMDTRGKVINCAF
                     AVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLVPKEDATAAKTEAE
                     AQS"
     gene            complement(33496..34683)
                     /gene="eutG"
                     /locus_tag="ACHKUC_000691"
     CDS             complement(33496..34683)
                     /gene="eutG"
                     /locus_tag="ACHKUC_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO6616997.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34673..35509)
                     /gene="eutJ"
                     /locus_tag="ACHKUC_000692"
     CDS             complement(34673..35509)
                     /gene="eutJ"
                     /locus_tag="ACHKUC_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO6616998.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRNGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(35520..>35610)
                     /locus_tag="ACHKUC_000693"
     CDS             complement(35520..>35610)
                     /locus_tag="ACHKUC_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6616999.1"
                     /translation="TTPTGEGVTSARTFVRLRRCVLVDAFRIV"
BASE COUNT         8318 a         9934 c         9072 g         8286 t
ORIGIN      
        1 cgatagttat tcgatatcga tatatttata ttttttcact attaataggc tgaaaaaact
       61 gtattggtac tctcctttcc cttgcattaa caatatgccg cttttaatca accttttatt
      121 aagcaatcaa ctaataacga tcggcatagg gaattatgct atgaaaaaac atctttttac
      181 tctgacactc tcttctgtat tggcgatacc agcagtatcc catgcagaat ttaaaggcgg
      241 ttttgctgat ataggtattc attatcttga ctggactagc agaaccactg agaaatcgtc
      301 gaccaaatca cacaaagatg attttggata tctggaactg gaaggcggcg ctaactttag
      361 ttggggagaa atgtacgggt tcttcgactg ggaaaacttt tataatgatc gtcatgataa
      421 accgggtagc gagcagcgtt atacatttaa aaataccaac cgtatttacc tgggtgacac
      481 cggatttaat ctctacttgc atgcgtatgg cacctacggt tctgcgaatc gtgtgaattt
      541 ccatgacgat atgttccttt acgggatcgg ctacaatttc accggcagcg gttggtggtt
      601 caaaccgttc tttgcaaaac gctatacaga ccaaacctat tataccggcg ataacggtta
      661 tgtcgccggt tgggttgcag gttacagctt tatgctgggc agtgagaaat tcaccctcac
      721 caactggaac gagtacgagt ttgatcgtga tgccacctac gcggcaggta atggcggcaa
      781 agaaggtctg aacggtgccg tcgcactttg gtggaatgca acttcacaca tcaccacagg
      841 gattcagtat cgttatgcgg atgacaaact gggcgaagat ttttaccagg atgcgataat
      901 ctactccatc aaatttaatt tctgattgat tactgccgga gctcccggca gtatgaattc
      961 actctatatc tgatacaacg gatccccttc ccgcccagcc attagcgcat tgccctggtc
     1021 tcgtactacg tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac
     1081 cagcatcaac tggcgttcaa ccactggcgt aatgcgcttt accaccagtg ggctaccttc
     1141 aggcaatggt agcgccagtg cgggaagaat actgatgccg atgcctgcgg ctaccatcgg
     1201 gaacagcgtc gccggatgac caatctcctg cacaatattc gcctgaatac cgttgcgcgc
     1261 cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc
     1321 tccctgtaat gcctgccagg ggacataatc ttccacggcc aaagcgctat cgcgatggca
     1381 aagcagaaag aaaggctcgg aaagaatcac ttcacattgc agatcgccca ctgggccagg
     1441 atcgatgacg atgccaaaat cgacatctcc ctggcgaata ctttccatca cccactgctg
     1501 cggacgatcg tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca
     1561 ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag
     1621 ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc
     1681 aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc
     1741 cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactca ctgccgactg
     1801 gctcaggccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa
     1861 aacctttaat tgctttaaag aataattcat ctgttttttt catgaatgga tgcaataaat
     1921 caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta
     1981 tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg
     2041 catctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg accactgcag
     2101 cgattgctct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg
     2161 gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga
     2221 ttctgggcgt gctgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg
     2281 gttttctcta cttgtgcatt ctccccgcga ccgtgcagtc tgcaatcgcc ttcacgtcaa
     2341 tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga
     2401 ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca gggggcagcc
     2461 ttgagcaggt cggtaaaatt atgctgcaac tgttgctgcc atttgtgttg gggcatcttt
     2521 cccggccttg gattggtgac tgggtgtcgc gtaataaaaa atggattgcg aaaactgacc
     2581 agacgtccat tctgttggtg gtttatacgg cattcagcga agccgtcgtt aacggtatct
     2641 ggcacaaagt tggctgggga tcattgctgt ttatcgtggt ggtcagttgc gttcttctgg
     2701 ctatcgtgat tgtagttaac gtctttatgg cacgccgact gggcttcaat aaggcagatg
     2761 aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa
     2821 acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc
     2881 agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt
     2941 tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga
     3001 ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct
     3061 tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcga
     3121 ttaagcgacg ccctttgtac ttgccaaacg gcattatcgt attggctatt tcaatcagct
     3181 gctctttttc catctcagct acccagcaaa cgcagcattt ccgtttcgtc gatgacttca
     3241 atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga
     3301 tcggttttct tcgacacgct gcccgcgact ttcgccccca gttcgaccag tcgagcttta
     3361 gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac
     3421 ggactgtcaa tctcttccgc attgataacg atcggctcag gccagtgaac accttccgcc
     3481 aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggag
     3541 gcaacgacaa ttccaacatc aggcaccttt tgcagctctt caatcgacgc ggcttccagc
     3601 gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tagcctcgcc aacttcacgg
     3661 ataccaagtg catagaggaa gcgggcaaag gtggtttctt tcgctttttc cagcgcgtta
     3721 accacgtttt gtgccgattt cggccccata cgctccagtc cggtcagttt gcctgcggtg
     3781 agtttgaaca gatccgccgg agtgtggaca tattcttttt caaccagttg atcgatgatt
     3841 ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagagactct
     3901 ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca
     3961 cgctcgacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct
     4021 tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga
     4081 ataaccactt tatcgccaat gcgtaaacca agacgttcga tttcgtcagc attatgcagt
     4141 gtagcgttac tcaccaacac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta
     4201 atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgttcctgc
     4261 gccgggaatt taaacgctac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc
     4321 tgctgtgcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcgga
     4381 cggtcttctt ctactttgtg gtagaacgcc agcacttctt ccgccgattc acaaaacgtt
     4441 acccgatcgc tgaccggcaa accccacttt ttaaattgca gtaaacggcc aagatgagta
     4501 tccggcagct cgccaccttc cagaacaccg acgccatagc agaagaaagt gagcggtcgc
     4561 ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta
     4621 gcaaacactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc
     4681 ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc gtgtagcttc
     4741 agcggaatgg cgcgaatagt acgcacatta gacgtaatat cttccccggt ggtgccatcg
     4801 ccacgcgtcg cggcactgac taaaacgcca ttttcataca gaatactgac ggcaagacca
     4861 tccagcttca gctcacagca ccaggtgacc ttctcgttgc ttttcagacg gtcctgcact
     4921 cgtttgttga aggcaagaaa gctttcttca tcaaaaacgt tatccagcga cagcattggc
     4981 acttcatggc ggatctggct gaaagccgcc agcggcgcag cacctacacg ttgggtaggc
     5041 gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc
     5101 ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga
     5161 tggcgaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat
     5221 caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta
     5281 tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg
     5341 agtcatcata cggcgctgat cgtcaagcac tacaccgccc acttcatcgg caatatgctg
     5401 cgcagattgc agcatcagct taaagttctg cagctcgtca ccgtaagacg gtacctgcat
     5461 aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt
     5521 caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg
     5581 gtaaatgttc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc
     5641 accgtttaac tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cctctttgcg
     5701 cttcggttta tccgtaactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac
     5761 gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca ctggctgcgg
     5821 cgctgacgcg acctgtggcg aaacaggctg ctgcaacggc tgttcaggct gcggctgata
     5881 ggcaggctgc tgcacaggtt gctgcaccgg ctgcgttgga cgcggagcat gttgcggcgg
     5941 tacctgcgct tcaggcggct gctggaccgg ttgacgcggc tgcgcagacg cataaggcgg
     6001 ttggtactgg tgttgcggcg acggacgagc aacctcatgc tcctgggcgt tagccggggc
     6061 atgattcacg cggtgaacac gaacctcacc aacgccctca tcatcttcga catcctcgtc
     6121 ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat ggccgatcgc ggaacataga
     6181 agatcgttct ttacggctgg tccagaaacc atgtaccagt aaagcgatta tggcgatcgc
     6241 gccaacaatg attaatatca gacgcaaatc ctgcatcatt atattctctg ttgttctaac
     6301 accttgccac cacggcaaac atttactcac taagagtatt tgccgattac ctcaagtgca
     6361 agtgcactat taactttcac agcacaaaga tagatgaaat cgtgcttttt gctgtttttt
     6421 cgaacatatc ctaactgtcc attgcgcaat tacccggtaa aatacgcaga attttcctgg
     6481 gattggtcaa aaggagctca tcctgattat ggtttcatca ttcacatctg ccccacgcag
     6541 cggtttttac tattttgcgc aaggctggaa gctcgtctcg caacctggga ttcggcgttt
     6601 cgttatttta ccgctgctgg tcaatatttt gttgatgggg ggcgcattct ggtggctctt
     6661 tacgcaactc gatgtctgga tcccgactct catgagttat gttccggact ggttacaatg
     6721 gctgagctat ctgttgtggc ccctggctgt catctctgtg ctgttagtgt ttggctattt
     6781 cttctcaacg attgctaact ggattgctgc tccgttcaac ggtttattgg ctgaacaact
     6841 ggaagcaaga ctgactggcg ctacaccgcc agataccgga attttcggta tcatgaaaga
     6901 tgtgccgcga atcatgaaac gcgaatggca aaaatttgcc tggtatctgc cgcgcgcaat
     6961 tgtattgcta attctttact tcatccctgg cattgggcaa accgtcgcgc cggtactgtg
     7021 gttcctgttt agcgcctgga tgttagccat ccagtattgt gattacccct tcgataacca
     7081 caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca
     7141 gtttggtgct ttaaccagcc tgtttacgat gatcccgctg cttaatctgt tcattatgcc
     7201 cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc tatcgcgata aacacgccat
     7261 gtggcggtaa caatctaccg gttattttgt aaaccgtttg tgtgaaacag ggggggctta
     7321 taccgcccct tattccatct tgcatgtcat tatttccctt ctgcatatag atatgctaaa
     7381 tccttacttc cgcatattct ctgagcgggt atgctacctg ttgtatccca atttcataca
     7441 gttaaggaca ggccatgagt aagatttttg aagataactc gctgactatc ggtcacacgc
     7501 cgctggttcg cctgaatcgc atcggtaacg gacgcattct ggcgaaggtg gaatctcgta
     7561 accccagctt cagcgttaag tgccgtatcg gtgccaacat gatttgggat gccgaaaagc
     7621 gcggcgtgct gaaaccaggc gttgaactgg ttgaaccaac cagcggtaat accgggattg
     7681 cactggccta tgtagctgcc gctcgcggtt acaaacttac cctgaccatg ccagaaacca
     7741 tgagtattga acgccgcaag ctgctgaaag cgttaggtgc aaacctggtg ctgaccgaag
     7801 gtgctaaagg catgaaaggc gcaatccaaa aagcagaaga aattgtcgcc agcaatccag
     7861 agaaatacct gctgctgcaa caattcagca atccggcaaa ccctgaaatt cacgaaaaaa
     7921 ccaccggtcc ggagatctgg gaagataccg acggtcaggt tgatgtattt attgctggcg
     7981 ttgggactgg cggtacgctg actggcgtca gccgctacat taaaggcacc aaaggcaaga
     8041 ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc agttatcgcc caggcgctgg
     8101 caggtgaaga gattaaacct ggtccgcata aaattcaggg tattggcgct ggttttatcc
     8161 cggctaacct tgatctcaag ctggtcgaca aagtcattgg catcaccaat gaagaagcca
     8221 tttctaccgc acgtcgtctg atggaagaag aaggtattct tgctggtatc tcttctggag
     8281 cagctgttgc tgcggcgttg aaactacaag aagatgaaag ctttaccaat aagaatattg
     8341 tggttattct gccgtcatcg ggtgagcgtt atttaagcac tgcattgttt gccgatcttt
     8401 tcactgagaa agaactgcaa cagtaatgcc agcttgtaaa aaatgcgtaa aaaagcacct
     8461 ttttaggtgc ttttttgtgg cctgcttcaa actttcgccc ctcctggcat tgattcagcc
     8521 tgtcggaact ggtatttaac cagactaatt attttgatgc gcgaaattaa tcgttacagg
     8581 aaaagccaaa gctgaatcga ttttatgatt tggttcaatt cttcctttag cggcataatg
     8641 tttaatgacg tacgaaacgt cagcggtcaa cacccgccag caatggactg tattacgctc
     8701 ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac aggctaaagt cgaaccgcca
     8761 ggctagactt tagttccaca acactaaacc tataagttgg ggaaatacaa tgttccagca
     8821 agaagttacc attaccgctc cgaacggtct gcacacccgc cctgctgccc agtttgtaaa
     8881 agaagctaag ggcttcactt ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc
     8941 gaaaagcctg tttaaactgc agactctggg cctgactcaa ggtaccgttg tgactatctc
     9001 cgcagaaggc gaagacgagc agaaagcggt tgaacatctg gttaaactga tggcggaact
     9061 cgagtaattt cccgggttct tttaaaaatc agtcacaagt aaggtagggt tatgatttca
     9121 ggcattttag catccccggg tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa
     9181 attgtcattg accggaaaaa aatttctgcc gaccaggttg atcaggaagt tgaacgtttt
     9241 ctgagcggtc gtgccaaggc atcagcccag ctggaaacga tcaaaacgaa agctggtgaa
     9301 acgttcggtg aagaaaaaga agccatcttt gaagggcata ttatgctgct cgaagatgag
     9361 gagctggagc aggaaatcat agccctgatt aaagataagc acatgacagc tgacgcagct
     9421 gctcatgaag ttatcgaagg tcaggcttct gccctggaag agctggatga tgaatacctg
     9481 aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc
     9541 ctgaagatta tcgacctgag cgccattcag gatgaagtca ttctggttgc cgctgacctg
     9601 acgccgtccg aaaccgcaca gctgaacctg aagaaggtgc tgggtttcat caccgacgcg
     9661 ggtggccgta cttcccacac ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg
     9721 ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact atctgattct ggatgccgta
     9781 aataatcagg tttacgtcaa tccaaccaac gaagttattg ataaaatgcg cgctgttcag
     9841 gagcaagtgg cttctgaaaa agcagagctt gctaaactga aagatctgcc agctattacg
     9901 ctggacggtc accaggtaga agtgtgcgct aacattggta cggttcgtga cgttgaaggt
     9961 gcagagcgta acggcgctga aggcgttggt ctgtatcgta ctgagttcct gttcatggac
    10021 cgcgacgcgc tgcccactga agaagaacag tttgctgctt acaaagcagt ggctgaagcg
    10081 tgtggctctc aggcggttat cgttcgtacc atggacatcg gcggcgacaa agagctgcca
    10141 tacatgaact tcccgaaaga agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg
    10201 atggatcgta aagagatcct gcgcgatcag ctccgcgcta tcctgcgtgc ctctgctttc
    10261 ggtaaattgc gcatcatgtt cccgatgatc atctctgttg aagaagtgcg tgcactgcgc
    10321 aaagagatcg aaatctacaa acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca
    10381 attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa caattgcacg tcatttagcc
    10441 aaagaagttg atttctttag tatcggcacc aatgatttaa cgcagtacac tctggcagtt
    10501 gaccgtggta atgatatgat ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac
    10561 ttgatcaagc aagttattga tgcttctcat gctgaaggca aatggactgg catgtgtggt
    10621 gagcttgctg gcgatgaacg tgctacactt ctgttgctgg ggatgggtct ggacgaattc
    10681 tctatgagcg ccatttctat cccgcgcatt aagaagatta tccgtaacac gaacttcgaa
    10741 gatgcgaagg tgttagcaga gcaggctctt gctcaaccga caacggacga gttaatgacg
    10801 ctggttaaca agttcattga agaaaaaaca atctgctaat ccacgagatg cggcccaatt
    10861 tactgcttag gagaagatca tgggtttgtt cgataaactg aaatctctgg tttccgacga
    10921 caagaaggat accggaacta ttgagatcat tgctccgctc tctggcgaga tcgtcaatat
    10981 cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt ggtgatggca ttgctatcaa
    11041 accaacgggt aacaaaatgg tcgcgcctgt agacggcacc attggtaaaa tctttgaaac
    11101 caaccacgcg ttctctatcg aatctgatag cggcgttgag ctgttcgtcc acttcggtat
    11161 cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt gctgaagaag gtcagcgtgt
    11221 gaaagttggt gatactgtca ttgaatttga tctgccgctg ctggaagaga aagccaagtc
    11281 taccctgact ccggttgtta tctccaacat ggacgaaatc aaagaactga tcaaactgtc
    11341 cggtagcgta accgtgggtg aaaccccggt tatccgcatc aagaagtaat tcttgccgca
    11401 gtgaaaaatg gcgcccatcg gcgccatttt tttatgcttc agccagaggc ggcaaaatca
    11461 attcatcgct ctcatgctgc tgggtatagc gcatcacttc cagtacgcgc aatcccgcgc
    11521 ggtgtaccgc atcggttaac gccttccctt tcagcaatcc actgataagc tgagcacaaa
    11581 acaggtcgcc agtccctttc aggtcagttt taacccggga atgggaaatg acattcacgc
    11641 tgtcggcact gaccaccacc acctgcatct cctgattttc ttcattgccg gaggcgctgg
    11701 taatcaccac ccactttaat gtgtctgaaa gcagactttt tgcggcagca atggcactgt
    11761 cgagatcgcg gcaatcttta ccggtcagga tttccagctc aaagatattg ggggtaattc
    11821 cctgtgccag cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt ttgacataaa
    11881 ttccgctatc aatatcgcca atcaccggat cgaccatgat caataggtca ggatggtctt
    11941 tgcgtagcgc agtcagccac tcggcaagga ttttgatttg cgatgccgtt cccatatagc
    12001 ccgtggttac agcacgaagt tggcgcagcg catcacgctc ctgaagcgca cgcaaataac
    12061 cgctaaacca ttcatccgga atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc
    12121 tcagcaatac cgtcggcacg gcaaagacat tcaggccgtt ctgctttata gcaggcaccg
    12181 caatgctgtt gcctacgctg ccgtacacta cctgcgactg cacggcgacg atatccgcct
    12241 gcagtgccct gctcttatcg ttaaacaaca acaaactact catttaattt tttctccttg
    12301 ccgatgatcc tcatcgtatt ccagccgaaa ctttacctga ttctggcagt caaatcggct
    12361 attacaaaac aaggataagg taattcagtg aagaaaatca tttgtctggt cgttacgctg
    12421 ctaatgacac tccccgctta tgcgaagtta acggcccatg aagaagcccg tatcaacgcc
    12481 atgctggagg gattagcaca gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat
    12541 acctgcgatg aagcggtttc tcatctgcgc ctgaagctgg gcaatacccg taatcgcatt
    12601 gatacagccg agcagtttat tgacaaggtg gcctcgtcgt catcgattac cgggaagccg
    12661 tatatcgtga agatgcccgg taagagcgat gagaacgcac agcctttttt acatgcgtta
    12721 attgcgcaga cggataaaac ggtgcctgcg caataatctt aaatccccgc cccctggcta
    12781 aaatgctctt ccccaaacac cccggtagaa aggtagcgat cgccacgatc gcagatgatc
    12841 gccaccacca ccgcgccagg gttagctttt gccacccgca gtgctccggc aaccgcgccg
    12901 ccggagctta cgccgcagaa aatcccttcc cgcactgcca gttcgcgcat ggtgttttcc
    12961 gcatcgcgct gatgaatatc cagcacctca tccaccagag aagcattgaa aatccccggc
    13021 agatattccg caggccagcg gcgaatgccg ggaatactgc tgccttcttc cggctgcagg
    13081 ccaacaatgg tcaccggttt ggattgttcg cgcataaagc gtgacacgcc ggtgatagtg
    13141 ccggttgttc ccatgctgga gacaaaatgg gtaatacgcc cgccagtttg ctgccagatt
    13201 tccggcccgg tagtggtgta atgggcgtaa gggttatcgg gattattgaa ctgatcgagc
    13261 aactttcctt cgccgcaatt cgccatctcc agcgccagat cgcgtgcgcc ttccatgccc
    13321 tgttctttgg tgaccagaat cagttctgca ccgtaggcac gcatcgccgc acggcgttcc
    13381 tggctcatgt tgtcgggcat cagcaatttc atgcgatagc ctttaagcgc cgcaatcatt
    13441 gccagcgcaa tgccggtgtt accactggtg gcttcgatca gcacatcacc cggtttaatc
    13501 tcgccgcgct tttccgcctc gacaatcatc gaaagcgccg cacggtcttt taccgatccc
    13561 gccgggttat tgccttccag ttttaaccac acttcactgc cgttatccgg cgccattcgc
    13621 tgcaacttca ccagaggcgt attgcctatt gtttgttcta atgtactcac gatctctgtc
    13681 catacgtggt gttgcctgat gcgacgctta cgcgtcttat cagacctaca ggttacaaaa
    13741 ccttgccatt aaaaaacccg gatcgcggga agcgcctccg ggcgtttaac attcactcaa
    13801 cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg ggtttcaata cgctcatcgc
    13861 cgttatacag ccgcgcatgt tgcagaccaa cgtataaacg atcgccacgc tgcggggcat
    13921 cgtcgccatg catcacgacc gtcagcggtt cgttgtacca ccccagcggc tgcaccacta
    13981 attgggtgta gtgacctttc gggctggctt ccagtacctg tacgggtagc ggcgaatcga
    14041 ggctggtacg gcggctgata tccacttccc acgggcgcag gaagagatcc accggcccct
    14101 gatacgcggg ggtgtatccc agcggccagc gatgtgcgcc aacgtggaac tgcccgccgc
    14161 gaatggttcc ctgcaggcgg tttacctcgc ccataaactc cagcacaaaa cgagtcgccg
    14221 gttcacgcca tacctgatcc ggtgcgtcag cttgttcaat attgccctga ctcatcacca
    14281 ctacacgatc agctacttcg gtcgcctctt cctggtcgtg ggtcacgaac acgctggtga
    14341 atttcagttc ttcatgcagt tgacgcagcc agcgacgcag ctctttacgc acctgcgcat
    14401 ccagcgcgcc aaacggttca tcaagcagca aaatttgcgg ttccacagcc agcgcgcgtg
    14461 caagcgccac acgctgtttc tgaccgccgg aaagctgcgc cggataacga tccgccagat
    14521 gagcaagctg caccatctcc agcaattttg tcactttcgc tttgatcgcc gccgcattcg
    14581 ggcgttcacg ccgtggcaac accgtcaggc caaaagcgat attgtcaaac accgtcatat
    14641 ggcgaaacag cgcgtaatgc tggaacacga aaccgacttt acggtcccgc gcgtgcagac
    14701 ggctcacgtc ggtaccgtga aagcgaatat gcccactggt ttgatgctcc agcccggcga
    14761 taatgcgcag cagcgtagtt tttccggaac cggacggccc aagcaacgcg accatctgac
    14821 cagaaggaat atccagtgag atatcgttca gcacctgggt gcgaccaaaa gacttcttaa
    14881 tattggcaat ctcaatgctc atgatgttcc tcctgctgcg cgcgtttttc ctgattttcc
    14941 aggcgccact gcaacatact ctttaaaaac agggtgataa tcgccatcag cgtcaacaac
    15001 gcggcggcgg taaaggagcc gacggtgttg tagtcctgct ccagcaattc aatctgtaac
    15061 ggcagcgaca gggtttcgcc acgaatcgag ccggaaacca ccgataccgc gccaaactcg
    15121 ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca gcgcccagcg gatgttcggc
    15181 agcgtgacgc gacggaacat ctgccagccg gacgcgccaa gtaaaatcgc cgcttcgtct
    15241 tcctggctgc cctgacttaa cattaccggt accagttcgc gcaccacaaa cggacacgtc
    15301 acgaagatgg tgaccagcac catacccggc caggagaaca taatttgcag attatgttca
    15361 tccagccagc cgcccagcgg gccgttagag ccgtagaaca gcagatacac cagaccagca
    15421 accaccggcg acacggcaaa cggaatgtcc agcagcgtca gcaatagctg gcgtccgggg
    15481 aagttaaagc gcgtcaccag ccaggccagc agaatgccga acaccaggtt taccggtacg
    15541 gcaatcagcg cgatcatcac cgtcagccag atggcgtgca gcatgtccgg atcggccaga
    15601 ttttgtaaca ccggcatcag tcctttgctg aacgcctgta cgaagatata aatcatcggc
    15661 accagcagga tgaacgccga taccagcatc ccgatgccaa tcagaaacca tttgccccag
    15721 ttaatcgggc gcgcgtcata acgcttcaat tgtgtcactt ccgccattaa tgacctacca
    15781 cacgccgacc aaagcgactt tgcagagtgt taattgagaa cagcaacagc agcgatgccg
    15841 cgaggatcac cgaggcaatc gcgctcgccg ccgggtaatc aaactcctgt aagcgaacaa
    15901 agatcatcag cgacgtcact tctgttttcc aggcgatatt cccggcgata aaaatcaccg
    15961 cgccaaactc accgaggctg cgggtaaacg acagcgccac acccgccacc agcgccggag
    16021 atagttccgg cagcaccact ttgcgaaaac tctgccagcg tgttgcgccg agcgtttctg
    16081 ccgcctcttc atattccggg cctaactctt ccagcaccgg ctgcacggtg cgcaccacaa
    16141 acggaatgct ggtaaaggcc atcgccaccg caatccccag ccaggtatag gtgactttga
    16201 tatcaaactt cgccagccat tcaccgtaaa aaccgttcac ggaaaagagc gaggccagcg
    16261 ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg
    16321 tgcgacccgg aaagcgatag cgggttaaga tccacgccat cagcagaccg aaaacgccgt
    16381 taaaaatcga tgccacaaac gccgacagca gcgtcacttt ataggctgcg accacctgcg
    16441 ggttggtgat cacctcccag tactgcgccc agctcatctg ggccagttgc atcaccagcg
    16501 cggagagcgg cagcagcaaa atcaggcaca caaacagcag actggtgccg aggcttaagg
    16561 taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa
    16621 cagcttgtct aactcgccgc cgctggtgaa gtgggttttc atcacttccg gccaggagcc
    16681 aaatttatct tccacgcgga acagctcggt ctgcgggaat ttatctttca gtttgcccat
    16741 gacttccggg ttatttacgc ggtagtaata gtcggtgatg atggtttgcg cctgcgggct
    16801 gtagagccag ttcaggtagg cttttgccgc tttttccgtg ccgttggcct gtacgttttt
    16861 atccacccac gccaccggga attccgccag aatgttggtt ttcggaatca ccacttcaaa
    16921 gccctgcgct tcatactgtt tacggatgtt gttcacttcc gactcgaagc tgatcagcac
    16981 atcgcccagg ccgcgctcgg caaaagtggt ggtcgcgcca cgaccgccag tatcgaacac
    17041 ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt ttggctttat caccaccgtc
    17101 ggctttgtcc gctgcgcccc atgccgcaag gtaggtataa cgcgcgttac ccgacgtttt
    17161 cgggttcggg aaaatcagct tcacgtcaga gcgtaccagg tcgttccagt cgtggatatt
    17221 cttcgggttg cccttacgca ccaggaagcc catggtggag tagaacggcg agctattatt
    17281 cggcaggcgc gactgccagt ccgctgggat cagcttgcct ttatcgtgca ggatttgtac
    17341 gtcggtcacc tggttataag tgacaacgtc ggcttttaaa ccctgcaaaa ttgccagcgc
    17401 ctgttttgat gatccggcat gagattgttt aatcgtcagt ttgtcgccac cgttatcttt
    17461 tgcccattgt tgctcaaacg gcgggttcag ggcggcaaac agctcgcggg agacgtcata
    17521 agaactgttc agcagttccg ttgcctgtac atggcccgcc agtagcagag aagcaaccag
    17581 cacgagtgag ttctttttca gtaagttaac ggccattgcg cacccttata aatttaatga
    17641 ctttctaata gccatcatat ttataacgga cgtgaaagga gtaacggttt tatataccgt
    17701 ttggtgattt ggaagttgaa aagggaataa gacgaaatcc tccccacaaa tgcagggagg
    17761 aaacagaggt gaatcagatg ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa
    17821 tcacattctg tgtaccggtt aaatagctgg attcatccga tgcgaggaag gccgccagtt
    17881 cgccgacttc cagcggatcg gcgaggcgac gcatcgggat tgctttcgcc atttcagtca
    17941 gcaccgattc tggatcttcc gggttcgact ggcgggcaat gctttccgcc attggcgtgc
    18001 gaacgtatcc agggcagatg gcgttaacgc gaataccaga ctgcgcgtac tccaccgcca
    18061 gcgatttagt caggccaaca atcgccgctt tcgttaaggc gtacgccgtt tcgcccgggt
    18121 ctgccaccat atcgccagtg actgaagaca tcatcacaat ccggccatct ttgcgggcaa
    18181 tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc ttttatattg atatcaatat
    18241 ggaaatcgcg atcttcatcg ctcatatcaa ggaaactgcc cagacgacaa acgcctgcgt
    18301 tattcaccag aatatcaatg cgcccttctt tttccttagc gcgcttaata gctgcggcta
    18361 ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca gcgatgacca cggccacaca
    18421 gttcgtccgc cagcttttcg atctcagggg agatatccag caagattagg tttgcgccat
    18481 gacgcgcaaa agttctggca attccttcgc caattccctg caatgcgccc gtaatcagtg
    18541 ctgtcttgcc cgtgagttta cccattttaa tgctctcctt gttaatacag agcgttacac
    18601 tccattccct ctgaaaatac aggataaaca aaatattgct gcggatcagc tcactaattc
    18661 aggaaaatct caatttagtc gctgctttat gcagcacctc aaacgttacg tcagcgcgca
    18721 atcattttca gtgacatcac atgagtaata ttcggactta atgattcctt tgtgtctatc
    18781 aaatcgggat atatgatccc atttatctgc aattatttga tcgcaaatat taaggtcacc
    18841 accgatgcaa cttgaaaaga tgattaccga aggctcgaac gccgcctcgg ctgaaattga
    18901 ccgcgtatca acgctggaaa tgtgccggat tatcaacgat gaagataaaa ccgtaccact
    18961 cgccgttgag cgcgtactgc cggatatcgc cgcggcgatc gatgttatcc acacccaggt
    19021 aagcggcggc ggccgtctga tttatctcgg tgcgggaaca tccggtcgtc tggggattct
    19081 ggatgccagc gaatgtccgc cgacctacgg cgtgaaaccg ggcctggtag ttggtttgat
    19141 tgctggcggc gaatatgcca ttcagcacgc ggtggaaggc gcagaagata gccgggaagg
    19201 tggtgttaat gatctgaaaa atatcggctt aaccgcgcag gatgtggtgg ttggtattgc
    19261 ggccagcgga cgcacgccgt atgtgattgc cgggctggaa tacgcgcgcc aactcggctg
    19321 ccgtacagtg ggcatttcct gtaatccggg gagtgccgtt tcatccaccg cagagtttgc
    19381 tattacgccg gtagttggtg ccgaagttgt taccggttct tcgcgaatga aagcaggcac
    19441 ggcgcagaaa ctggtgctca acatgctctc caccgggctg atgattaaat ccggcaaagt
    19501 gttcggcaac ctgatggtcg atgtggtcgc caccaacgaa aaactgcatg tgcgccaggt
    19561 caatatcgtc aaaaacgcta ccggatgtaa tgccgaacaa gcggaagcgg cgttaattgc
    19621 ttgcgagcgc aactgtaaaa cgaccattgt gatggtactg aaaaatctcg atgccgatga
    19681 agctaaaaaa tgcctggatc aacacggcgg ttttattcgc aaggctctgg aaaaggaata
    19741 acccatggct aaagagatca gcagtgaact tctgaacacc attcttaccc gggtcggcgg
    19801 accgggaaat atcgccagtt gtggtaactg tatgacgcgc ctgcgtctgg gtgtacatga
    19861 cagttcattg gttgatcccg atatcaaaac gctggaaggc gtgaagggcg tcattttgac
    19921 cagcgatcag gtgcaggtgg tttttgggcc aggcaaagcg catcgtgcag caaaagccat
    19981 gagcgagctg ctgggcgagg caccggtaca ggatgccgca gaaatcgccg cccagaataa
    20041 acgccagtta aaagccagac aaacttccgg cgtgcaacaa tttctcgcca aattcgccac
    20101 catcttcacg ccgctgatcc ccggttttat tgccgccggt ctgctgctgg ggatagcgac
    20161 gttaattgcc acggtgatgc acgttccggc agatgctcag ggcacgctac ccgatgcgct
    20221 gaattttatg aaggtgttca gcaaaggcct gttcaccttc ctggtaattc tggtgggcta
    20281 taacgcggcc caggcatttg gtggcacggg cgtaaatggc gcaattatcg ctgcgctctt
    20341 tttgcttggt tataacccaa ctgcaaccac gggttactac gccggatttc acgatttctt
    20401 tggtctgccc atcgatccgc gcggcaatat tatcggcgtg ttgatcgccg cgtgggcctg
    20461 cgcccgcatc gaaggcatgg tgcgccgctt tatgcccgac gacctcgaca tgctgttgac
    20521 ctcattaatc accctgctga tcaccgctac gcttgcgtac ctgatcatta tgccgctggg
    20581 cggctggctg tttgaaggta tgtcatggct gtttatgcac ctgaacagta atccgctcgg
    20641 ttgtgcggtt ttagccgggc tgttcctgat cgccgtggta tttggcgtgc atcagggctt
    20701 tattcctgtt tatctggcgt taatggacag ccagggattc aacagcttat ttcccatcct
    20761 ttcaatggca ggcgcaggcc aggtgggcgc ggcgctggcg ctctactggc gggcgcaacc
    20821 gcacagtgca ttacgcagtc aggtccgcgg ggcgattatt cccggtctgc ttggcgttgg
    20881 cgaaccgctg atttacggtg tcaccctgcc ccgcatgaaa ccgtttgtta ccgcctgttt
    20941 aggcggcgcg gcgggcggtt tgtttatcgg cctgatagcc tggtggggtc tgccgatggg
    21001 cttaaacagc gcattcgggc cgtctggtct ggtggcgctg ccgctgatga cttccgcgca
    21061 aggcatcctt ccggcaatgg cggtttacgc tggcggtatt ctggtggcat gggttagtgg
    21121 gtttattttc accacactct ttggctgccg taacgtcaat ctggactgat tatgaaacgg
    21181 acaatgctct atctttctct gctggctatt agctgtagcg tcagcgccgc aaaataccct
    21241 gttctgacag aaagtagttg atcaagtgta tgcggcgtta aagcagtaat aaaaaggcca
    21301 gtcagttgtg ttactggctg gcctttaatc aatgaatcag aaacgcttac agcgccatca
    21361 acttgtccag cgacggcgcg aagtaatagc cgccagtgac cggtttggtg aaacgcaaca
    21421 tcgcatcacg cttaccatct gtatcgccaa acatgctcag cagttgctgc tcaatgttat
    21481 gcagacgcgc gcagtaggcg cagaagtaca gaccgtgagt gccactggcg gtgccgtacg
    21541 gcaggctctg acgaacaatc ttcagccctt tgccatcttc tttcagatca acgcgggtca
    21601 ggtgagaggt ttccggacgt tcgtcgccgt caatctcttc gttggcctct ttggtgcgcc
    21661 cgatcatcat ctcttgatcg tgaacgctca tccgattgag ctgtttcagg ttgtgctccc
    21721 aacgctgcac aaacacatag ctgccgcccg catccacgcc gtctttgata acagccactt
    21781 cgcggcgcgt ctcttcaccc gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac
    21841 gctcttcaac ccaacggaag ccgtggatct cttctttcac ttcaatgcag tcaccaaagg
    21901 cttccatcgc cgcctgagcg acagagaagt tcacgtcgtg acgcagagaa agaatgtgga
    21961 tcaacacatc gaactgggtc gtcggtgcaa gacctttacc gtagcccgga aaatctttca
    22021 gctcttcggc cccaacgccg ccgctcagag cgcgccaagt attgttacca aaggcaacca
    22081 ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg
    22141 ttttactggc cgcacgcagg gcgtcaactt cccctttcac gttggcttcg atccaaatcg
    22201 ccgcgcggca atgttctggc aaaatgccac tctgaacctg agacattgtt cctcctgaaa
    22261 ataataatgc cacgcaatcc gtggcattgt tgggcgctat tttacgtgtt ttttcgcggc
    22321 ggcggtttgt tcagacgcaa attaacgccg ccagataatt ttactgactt tccagttttt
    22381 cagggtatcg tccgaaggca ttaacccttc agggccactc cattccccgc tgaactggta
    22441 gctaatatgt tgactgcctt cggctttaca ttcgacggca cgattatcat cactgtcggc
    22501 cttttggcaa ttgccaaatg ctttgctgta aaggtcacta aacggtgtac cgattttaac
    22561 accggtgtca gcaggaatat cgctatccag cacatcaatg cggctgatcg tgccttgatc
    22621 accgttaatc accatcgcca cgttgtcgcc tttcatcact tcaaaaaagc gcaccacgtt
    22681 gccgttcgcg gttttcattc cgctgcgcag gcgataatcg ccatcaagcg catcagcaat
    22741 ggcttgttct tgcagtggtg tggacgccgt taattcaccc acgccctgct cgctcacttt
    22801 ggtggatgat ccaaaccagt tccacgggtt agcggccgac cagttaactg acgacatcgt
    22861 ggaacagccg gtcagcatca acggcatagc gcataacatt aaacgcagcg atttcatgtc
    22921 acttcctttg gttattcaat aacgttgatt ggagtgcaaa ttcaccaaaa agtgccgtta
    22981 ttctttttct tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc
    23041 cacgatatcc gccactacca gcgccagacc gataccgcta acggattcac cgttcagccg
    23101 cagccacggt tgccaacaaa gtaaaaccac ttgcgccaac agcaacagaa aatagagcac
    23161 atgccagata cggggaaata aagcccgccg accactcagc aaaaatgcca gcaccgccgg
    23221 aataccggga atcaacccca gccagaaatt atcgtgatcg ggataaaaca gattcagcag
    23281 cgcagtaccc tgctcgcgcg acgctccggc aatgacaaac agcacccagg ttcgcgcctg
    23341 aagcaatagc acaagccaga agagcaaggg taaacgcagg cgaccgtgcg catcataatg
    23401 gacaggatga aactcagtac tcttcatctt caatcaaacg cttacccaga ctcaacacgt
    23461 cggcgtgttc atatcccagg cgttcataca ttccgagcac catgtcgtta tcttccggca
    23521 cgttgatctg aattttcgga cagccgcgag caatcagctt tttctccagc cgattaagca
    23581 acgcattagc aatcccacgc ccacgaaact cgggatgcac gccaagataa taagcagacc
    23641 cgcgatgccc gtcataaccg cccatcaccg ttccgaccac ttcaccgttc acctcagcga
    23701 ccagaaacaa actgacgtca tggttcatct tacgctcgat gtccatttcc ggatcgttcc
    23761 acggacgcag caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc
    23821 gaaatacgcg tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg
    23881 cgtgaacggt cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg
    23941 gagttgtcaa ctattgaaat gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg
    24001 attacgcgat acgatataac atctggaact ttattattac aactcaggcc gtatgagcac
    24061 ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagccg gtttggctgc
    24121 cctgacgttg tcaggaatgt cgcaagccat cgccaaagaa gaaccgttaa aaaccagtaa
    24181 cggacacagc aagccgaaag caaaaaaaag cggcggcaga cgtgtcgttg ttctcgatcc
    24241 tggccacggc gggattgata ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt
    24301 ggtgctggca attgccaaga atgtccgttc catattgcgt aatcatggca ttgatgcgcg
    24361 tttaacgcgt tctggcgata cgtttatccc actgtacgat cgcgttgaaa tcgcccataa
    24421 acatggcgca gatctgttta tgtcaattca tgccgacggc tttaccaatc cgaaagctgc
    24481 cggtgcttcg gtatttgccc tctctaaccg tggggcaagt agcgcaatgg caaaatacct
    24541 gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa aaagcgactg acaaggatca
    24601 cctattgcag caagtgctgt ttgatctggt gcaaaccgat actattaaaa acagcctgac
    24661 gctcggctcg catattctga aaaagattaa gccggtgcat aaactgcaca gccgcaacac
    24721 cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt ccgtcggtgc tggtggaaac
    24781 ctcgtttatc accaacccgg aagaagagcg gctgttaggc actgcagcgt ttcgccagaa
    24841 aatcgccaca gcgattgccg aaggcgtgat cagttatttc cactggtttg acaaccagaa
    24901 agcacactcg agaaagcggt aagtgatgaa acccgacgca caccaggtta aacagtttct
    24961 gctcaacctt caggatacga tttgtcagca gttgagcgcc gtcgatggcg cagaatttgt
    25021 cgaagatagc tggcagcgcg aagctggcgg cggcgggcgt agtcgggttt tgcgtaatgg
    25081 tggtgttttc gaacaggcag gcgtcaactt ttcacatgtc cacggtgagg cgatgcctgc
    25141 ttccgccacc gctcatcgtc cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc
    25201 gctggtggta catccgcata acccgtatgt ccccaccagc cacgcgaatg tgcgcttttt
    25261 tattgccgaa aaaccgggtg cagagcccgt ctggtggttt ggcggcggct tcgatttaac
    25321 ccctttctat ggttttgaag aagacgccat tcactggcac cgcaccgccc gtgacctgtg
    25381 cctgccattt ggcgaagacg tttatccgcg ttacaaaaag tggtgcgacg aatacttcta
    25441 cctcaaacat cgtaacgaac agcgcggcat tggcgggctg ttctttgatg atctgaacac
    25501 gccagatttc gaccactgtt ttgcctttat gcaggcggta ggcaaaggct acaccgacgc
    25561 ttatttgcca attgtagagc gacgaaaagc gatggcctac ggcgagcgcg agcgcaattt
    25621 tcagctctac cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc gcggcacgct
    25681 gtttggcctg caaactggcg ggcgcaccga gtcgatcctg atgtcaatgc cgccactggt
    25741 acgctgggag tatgattatc agccaaaaga tggcagccca gaagcggctt taagtgagtt
    25801 tattaaggtc agggattggg tgtaactccc tcacccccac tcccgcatcc gctgatgcag
    25861 cgtcagtgac ggcttctcgg caaacagttg ctgataatcc gtggcaaatt gcccaagatg
    25921 ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgtgacc acggacttat
    25981 gagttcgcgg cgcacggcgt tcaggcgaat acgtttcagc cacgcgttcg gaccaatgcc
    26041 caaaatggcg tgaaacgcgt tttgtagcgt gcggcggctg acatgcagtt gattacacaa
    26101 gtccagcacc gtcaccggtt cggacatgtt ttccagcaca tattcacggg cgcgggaaag
    26161 caaccgacgg taactctgat gactgatgct ttccgccgtc accattggct gcgcttcttc
    26221 cagcatcgcc cccatcgcca ttagcaaatt atcccccagc actttacgca cagcaggctg
    26281 atgaagattt tccgggttct cgcaaaatgt cgccagcgcc tgttggacaa agccccacag
    26341 cgcggcttta tgctgctctt tcacttccaa cgccgactgg ctacgcagca tatgtaatac
    26401 ccgatccgga ttatgcaaaa agttagcctg acgggtgatg acatcttcag aaagcactac
    26461 gccaaggatg gtgtaatcgt ccggcgtact taattcaaac tcggttccac cagggcgcgt
    26521 cgctatttct gcgcttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc
    26581 cggaatgcca aaccagaacg aattcggcca gaccaggcac gactgacgca gcgccagacc
    26641 ggtgtattca cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt caccatgaaa
    26701 cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg gtaattgtca aagcatgttc
    26761 atagacgtcc gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt
    26821 caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt
    26881 catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt
    26941 gagacgataa cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc
    27001 atttctggct ttttcgagcg gccagccaaa gtgggctgcc acttctcctg ccgtcattcc
    27061 ctgacgtact gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg
    27121 cgcgtcgggt tcctttacgg gttgcttagg ctggcggtta aagccagtaa ccagccactg
    27181 ggtgtcatcg tctggccgac cgatctcctt gcggctgatg acacgcccgg tacgcatcgc
    27241 ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag
    27301 cgttaggcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag
    27361 catggcatca gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc
    27421 attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac
    27481 tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc
    27541 tgccgagtag ttggtttcag acggtggcgg gacataggtc accagctgca cgtcggcaga
    27601 tttcaacgct gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat
    27661 cggatcgcca agcgtgatcc cggcggttga tgagagataa gaaccggtac gcgaaactac
    27721 atgtgccagg aaggctgtgt tttctgcgtc attagcccac tggaaagcag cgccattttc
    27781 aatattcgca accatcgcat ccagaccagc acgcacttcc gccgggtttg gtccaccaag
    27841 cataatcagc acctcaccgg cagtcggtga cggaccgtgt gcagcgcccg catacagcga
    27901 tcgaccatat accacttcga ccatcgcctg cttggtcgct tcgtcagccg caatgtaggt
    27961 gacgtcatct gaatcagcag aaatcagccc gaggctacga atatgcggcg gcaatttcag
    28021 ttcacgtgca aattcggcgt taacggaggc aatcacccgc atggcggtta ctgacggtcg
    28081 aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt
    28141 ctgctccagc atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt
    28201 gccgccctgg tgaatgttag agatacaggt gcgatcggcc tcaacggtgg tcgccatacg
    28261 cggcgagtaa acggcgtagc aggagaggct ttctgactgc cccaaccccg gacgttcgcc
    28321 caccagcagg atcactactt tcgcgcctag gatctcaccg atctgatctt caatcttcac
    28381 gcgaccataa cgtacaaaga atggcgtacc gactttcagc ccggcttgct tcaggcccgc
    28441 catcagcggc ggcaggatct cttcgtagtt cacggtgatc gcatcagttg acaggccatc
    28501 agaaataacg acctgcacgt ccggattagc aacacactgt gctttcagcg cttcaacagc
    28561 ttctgcacac aggcggcggc ccatatccgg acgagtcagg tagagatttt tgtcgctgat
    28621 ttcggagcgg acttccagca agccctgcgc tttcacccac tcttccggta cttctttcag
    28681 tacggtatct ttcgaacgag agtgatcggc caggaaacgc agcaacgcct gggtacgcgg
    28741 acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa
    28801 tacgtccgcg cgatgtgggt tttccacacc aatccacgct tttgcttccg cagaacctaa
    28861 atccagcgcg cagctttccg aggtcaccgg tgccgcacag ttggtggtgg cgcactttgc
    28921 ttctgacggc gcgggggccg cttgtcccat tgacgccatc acgctgcgta caatttcttc
    28981 aatctgtttt tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg
    29041 cccgcccgtt tggtcaggcg accgtttgcc ataatgccca tgctttccag ccagcgttca
    29101 aactccggtg acggacgcag attcagtaac tgacgcacag tggcagtgtc gtggaatgcg
    29161 gtggtctgat agttgagcat gatgtcatcg cccagcggca tccccatgat gtagttacag
    29221 cctgcggtgg cgagcaggat catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg
    29281 ttggtgtagc agcagtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct
    29341 tctaagcctg cgcggataat ctggcggtcg ttgtagagat actccggccc aataaaaccg
    29401 accacggtgt tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt acgcgcttcc
    29461 atcgtcacct ggtctgcgcc gaagttagcg ccagcggata gcgccgagcc ttgtccggtt
    29521 tcgaagtaga gacagttttc cccggcgata cggttgaact ccgcgcccac tgcgcgtgct
    29581 tcatcgagca tcgccagctc tacaccaaac tctttcagcc ctttttcgct gccgcagata
    29641 ctctggaaaa tcagcccgcc tggtgcgcca cgacggatcg cttcgatctg ggtggtgacg
    29701 tgcgccagta cgcagccctg agttgggatg ttgaatttgt cgatcacgcc ataaatggta
    29761 tccaacacgc ggcttaagtt ttccacgtcg tcagtcaccg ggttaacgcc aatcaccgca
    29821 tcgcccaccc caaaggaaag cccttcgtag atttgcgcgg cgatactttg cacgtcgtca
    29881 cgggtatcgt tcggctgcaa acgggcgcta aaggtgcccg gaataccgat ggtggtattg
    29941 gcctttttga tcaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc
    30001 ttcgctaccg ccgccaccac ttccgaggtc aaccctttgc gagtaaaggc aatgtcgtca
    30061 acgctggttt cgtcgctcag gacgtattca cgcagttcgc tgatgctcca gtttttaatc
    30121 tggttgtagg ccgtttcgtt aacgtcgtcc tgaatcagcc gcgtcacgca gtcatcttca
    30181 taggcaatca ccggattatt gcggatgtct gctacggtca tttccgacaa cacctgcttt
    30241 gccgccacgc gctcctgtga gcttgccgca gcaacgcccg ccagcacatc ccccgaacgc
    30301 agttcgttgg ctttagccag cacctctttt acatccttaa actgatatac attgccgaac
    30361 aatgtggtct ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg
    30421 tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc
    30481 ggacaatcac ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca
    30541 tgcccagagc tttaccaaag tcctgcccgg ccaccaccag caggggatgc ggattcggaa
    30601 aacgcgcgac gaaatcgacc agcgcgttga tgaccgtcag taccgcggcg taacgcacag
    30661 gcagcgaggc gggaagcgcc agcacgtacg cgtcagtttt ggggcaaaga tccagttgaa
    30721 tcagcgcctg ttgccaggca ctgaccagat ccgtttcatc aatcgggatc gccactggca
    30781 aattgcgcag cggcagttgt acgccctcca gccagattgt gctgccagag agcgaaaggg
    30841 tatgagcacc cgcgccaatc accgtggcgc gcacggtttg cgccggaaac tgcacgttca
    30901 tctcacgcag acgcggatgg tcatgcagcg ccgttgccag aagcgggccg atatcggcaa
    30961 aacagaacgg gtcggtgggc tggtggcgat aacattcgcc taccccgcca gaaagcgtaa
    31021 tgatttcggg cgtaacacct gcgggcagca aaccggtttg catcaatgcc tgcgcgagcg
    31081 gcgagagcgt tccgtcaatc acttcgacaa tcagctctgc catccggcgg gtaacctgca
    31141 ccagctgccc gccagtcagc gaacgggcgt cagtaccggc accgaagcac tcatccacaa
    31201 tcatctgccc cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt tccagcaggc
    31261 gaccgccgac gttgaggcag gcagtgccgc tgatttttcc ggcatcgaac agggcgtagt
    31321 tcgcggtgcc accgccgata tcgatattca ggacccgaca cagccgttgt tcagaaaggg
    31381 tttgcgcccc ggcaccgtgt ccggcgatca cggattcaag atgcggcccg gcgctggcga
    31441 cgacaaaatc gcccagcgac cgggagagcg ccatcaccgc cgggcgagcg ttgcgggttt
    31501 ttgcgctttc accggtgata atgatggcac cagaatcaac gctttccggc gcaatgcccg
    31561 cagcctgata ttgctcgagg attaaggttt tcagttccgc ttcttttaaa ccaccctgtt
    31621 tatcgacagg ggtaaagaac accgggcttt gccagctaat ttcgcgtttg atgaattcgt
    31681 agcgcggcac ctgcgacacc gccgcacggt taaccagctc caggtgggag aagatcacct
    31741 gggtggtggt ggtgccgata tcgataccga cgctcagtag ctggcgagtg ttcacgattg
    31801 cgcctccgct tcagttttag ccgcggtcgc gtcttctttt ggcaccagca tcatcgccac
    31861 gccaattgcc gttacgccgc cgatcaattt gccgacaatc atcgggaaga tcatggcgtt
    31921 catgttggca gcggcgaagc ctaaatggtc gcccagggcg aaagcagcgg aaacggcgaa
    31981 ggcgcagttg atgactttgc cgcgggtatc catctgcttc atcatgccaa acatcggaat
    32041 gttgttggca agcgttgcca ccatgccggc tgccgcgata ttgttcatat tcagcacctt
    32101 accgacgctc atcagcggtt tttcaaacca gcgagtcagc agcagtacca ttggatacgc
    32161 ccctaacaga acgcaggaga tcgagccgat aacttcaatg gcgcgcatca cttcaccggg
    32221 tttatcgcca ggggccataa agataggatc aagacccggg atcagttccc agccaaggag
    32281 gaatttcact accgcagcgg caagaccgag ggtgatcaat gcaacgagga atttggcgaa
    32341 gatctggaag ccgttgatca ttttttccgg gatgaatttc agccccagcg ccaccagcac
    32401 cgcaacgata agtaccggga tcatgttcat caggatcagc gcaaaggtga attcaacagg
    32461 ctggccgttg atctgcacac cggagtacat cgctaccaga ccaccagcga tacagccaat
    32521 cggaatggtc actatgcccg ccagcacgcc gagcgccacc ggaatggaaa acacaatcgt
    32581 tggccccatc atcgacccga gaattaaccc agagtatagc cacgctgcta cgtcaccgcc
    32641 cgccagctct ttggcgagga agaagccgcc catatcgcac gccagcagtg ttccggcgaa
    32701 cattgacggg tttgcgccga gcatttcgta aaccgggata atcaccggtc cgagaacgtg
    32761 ggccagtacc ggtgccagtg cggtcatacc gaccatcgcc aggcccagag cgcccattgc
    32821 cataaagcct tcttcgaact gaccgccgga tccttcgata cttttaccga acttaccgag
    32881 gaaacgagca gaaccgccga actgcgacag gatcctgtct acggcagcta tcagcataaa
    32941 gaacatcatg atgtacatga tgatttcgtt aattcccatc gcctttactc cctgttagtt
    33001 gttatttatt ggcggatgcg gcgtgaacgc cttatccgcc ctacatgtgc aatcccgaag
    33061 gccagataag acgcggcaag cgtcgcatcc ggcaattgca ccgcgccact ggcggatgcg
    33121 gcgtgaacgc cttatccgcc ctacatgtgc aatcccgtcg gccggataag acgcggaaag
    33181 catcgcatcc ggcaattgca ccgcgccact ggcggatgcg gcgtgaacgc cttatccgcc
    33241 ctacatgtgc aatcccgtcg gccggataag acgcggcaag cgtcgcatcc ggcaattgca
    33301 ccgcgccact ggcggatgcg gcgtgaacgc cttatccgcc ctatatgtgc aatcccgtcg
    33361 gccggataag acgcggcaag catcgcatcc ggcaattgca ccgcgccact ggcggatgcg
    33421 gcgtgaacgc cttatccgcc ctacatgtgc aatcccgtag gccggataag acgcggcaag
    33481 cgtcgcatcc ggcatttatt gcgccgctgc gtacaggccg acaatctgct ccaggctggc
    33541 ggtacgcggg ttactgcgca gacaaatatc ttccagcgcg gcctgcgccc atgcgccgta
    33601 atgcgcagat gtcgcaccaa catcgcccag tcgtttacca atcccaactt ccgctatcag
    33661 ctcacttacc gcattaatag cgtcacgatc gtcggatttt ttagttcgca gcgcccgacc
    33721 aacctgacta aagcgttcac gacagaccat ccggttgaac tccatcaccg ttggcagcaa
    33781 catggcgttc gccagaccgt gcggaatatg cagcgccgcc cctggctgat gcgccatcgc
    33841 gtggcataac ccaagccccg cgctggaaaa cgccattccc gccatacatg aagccagcaa
    33901 catgctctcg cgcgcggcaa ggtcatggcc gtagcccacc gctttcggca gtgatttgcc
    33961 aatcatcgca atggcaccaa tcgccaggct gtcggtaaac ggtgtagcgt tcagcgcgct
    34021 gtacgcttca atggcatggg ttaacgcatc aatgccagtc atcgccgtga catgcgacgg
    34081 cacgccttcg gtcagcacag cgtcgagaat cgccacatcc ggcatcagcg aggcgtgggc
    34141 taacacctgc ttgcgcccgc tcaccgcgtc gataatcacc gtcacgttag tcgtttcaga
    34201 gccggttccg gcggtcgttg gaatggcaat caacggcagg cgcggttgca gaacgctggt
    34261 ttctgacatt tctgccagcg tgctatccgg gttcgtcacc agcaacgcca cggctttcgc
    34321 cgcatccagc accgagccgc cgccaaacgc gatcaccccg tcacaacctg attcacgcaa
    34381 ctgcgccacg gctgcacaca cgtcggtgat gcacggttcg cccaccggac atggccagag
    34441 cgtcatggcg atacctttaa cggccaggct acgtgtcagc ccggcggtca tccctgcctg
    34501 atgcaaaaag ctgtctgcca tcacgaacag atgtttcaac ccacgcgttt gcgcttgctg
    34561 cccgcaactg ctcaccgcgc ccggaccgca aagcgtcacc ggtggaacgc taaatgtttt
    34621 tacccgttgc agattcaggg tatcgaacgc ctgaaaaagc gccgtttgca attcactttg
    34681 catagatccc ctccgctttc tctcgcccac tactggcgat tgccagcggg gtcataaaca
    34741 aactgtgctg cggtaaatgc acctgtaacg ccggaaattg tttgcaaaac agcgccgcta
    34801 cgcccggttg catacaggaa ccgcccgcca gccataaatc cgtaatgccc tgcccttcaa
    34861 tatgccgggc cacaatgtcc gccatttttt cgtacaccgg tttcaccgcg ggccaaatct
    34921 cgtcaccgtg accgcgtttg tactgctctg cctcttccag cgaaatacgg cgatttccgg
    34981 cgagagtcag agagatgtga tgtccgccgg tggcttcatc cgccgagtac gtcactttgc
    35041 cctttttcac gatggcaatg ccggtagtgc cgccgccgat atccactaca ccggcgttgt
    35101 ccagttgcag cagatccgca actgcggttg gctcatccag cacatggctc acttccagtc
    35161 cggcagactc cagcacgttg atggaaatac gcgggtccgt accaggagga aatgaggtcg
    35221 ccgcatggct aaaacggcga ccgaattgct gctcaagggt gtcgagatgg cgacgaacaa
    35281 tggtgaccgc accgaagaaa tcccagacga tgccgtcgcg gaccacatcg gcccagtcga
    35341 ggcataccgc caccggctga ccgttgcggt cgacaaccat cgacaccaca tcgcaagtgc
    35401 ccagatccac ccccagccac agcggagatt ccgtcgcggc aggcgtctgg ttacacagcg
    35461 ttgccgccgt ttgcagacgt ggggtgagcc attgttcatc gtgcgccatc ggttactcct
    35521 taaacaatgc gaaacgcatc gactaataca cagcgacgca gacggacaaa cgtccgcgcg
    35581 ctggttaccc cttcaccggt tggcgtggtg
//
LOCUS       DAZFRG010000018        35151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFRG010000018 DAZFRG010000000
VERSION     DAZFRG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.018"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            492..1580
                     /gene="pdeL"
                     /locus_tag="ACHKUC_000694"
     CDS             492..1580
                     /gene="pdeL"
                     /locus_tag="ACHKUC_000694"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO6617000.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1622..2554)
                     /gene="yahB"
                     /locus_tag="ACHKUC_000695"
     CDS             complement(1622..2554)
                     /gene="yahB"
                     /locus_tag="ACHKUC_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6617001.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLITRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2646..3143)
                     /gene="yahC"
                     /locus_tag="ACHKUC_000696"
     CDS             complement(2646..3143)
                     /gene="yahC"
                     /locus_tag="ACHKUC_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6617002.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLAPVSGISGVWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3401..4006
                     /gene="yahD"
                     /locus_tag="ACHKUC_000697"
     CDS             3401..4006
                     /gene="yahD"
                     /locus_tag="ACHKUC_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6617003.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGANINKQDHTCLNPFLMSCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4046..4909
                     /gene="yahE"
                     /locus_tag="ACHKUC_000698"
     CDS             4046..4909
                     /gene="yahE"
                     /locus_tag="ACHKUC_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6617004.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIVLPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITIGQHHHIEMSHCQRWLSPTMQMTAL
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     AVAVASMMGLGIGLTPSADDYLTGLALILFLPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4899..6446
                     /gene="fdrA"
                     /locus_tag="ACHKUC_000699"
     CDS             4899..6446
                     /gene="fdrA"
                     /locus_tag="ACHKUC_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6617005.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLADIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSAEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6446..7863
                     /gene="yahG"
                     /locus_tag="ACHKUC_000700"
                     /pseudo
     CDS             6446..7863
                     /gene="yahG"
                     /locus_tag="ACHKUC_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
     gene            7887..7967
                     /gene="yahH"
                     /locus_tag="ACHKUC_000701"
                     /pseudo
     CDS             7887..7967
                     /gene="yahH"
                     /locus_tag="ACHKUC_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            8017..8967
                     /gene="yahI"
                     /locus_tag="ACHKUC_000702"
     CDS             8017..8967
                     /gene="yahI"
                     /locus_tag="ACHKUC_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO6617006.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8977..10359
                     /gene="yahJ"
                     /locus_tag="ACHKUC_000703"
     CDS             8977..10359
                     /gene="yahJ"
                     /locus_tag="ACHKUC_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6617007.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGPSVPLAHAGGAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFKCEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10576..10650
                     /locus_tag="ACHKUC_000704"
                     /pseudo
     CDS             10576..10650
                     /locus_tag="ACHKUC_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10737..11788
                     /gene="yahK"
                     /locus_tag="ACHKUC_000705"
                     /pseudo
     CDS             10737..11788
                     /gene="yahK"
                     /locus_tag="ACHKUC_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
     gene            12410..12653
                     /locus_tag="ACHKUC_000706"
                     /pseudo
     CDS             12410..12653
                     /locus_tag="ACHKUC_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12670..13344)
                     /gene="yahN"
                     /locus_tag="ACHKUC_000707"
     CDS             complement(12670..13344)
                     /gene="yahN"
                     /locus_tag="ACHKUC_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO6617008.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCGEIFSLIRIVGGAYLLWFAW
                     CSSMRRHSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPT
                     WARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASWVIGAIIGVFALRLIY
                     EGVTQR"
     gene            13491..13766
                     /gene="yahO"
                     /locus_tag="ACHKUC_000708"
     CDS             13491..13766
                     /gene="yahO"
                     /locus_tag="ACHKUC_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO6617009.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(13867..15453)
                     /gene="prpR"
                     /locus_tag="ACHKUC_000709"
     CDS             complement(13867..15453)
                     /gene="prpR"
                     /locus_tag="ACHKUC_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO6617010.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQASETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            15692..16582
                     /gene="prpB"
                     /locus_tag="ACHKUC_000710"
     CDS             15692..16582
                     /gene="prpB"
                     /locus_tag="ACHKUC_000710"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO6617011.1"
                     /translation="MPLHSPGKAFRAALSKETPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAEAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            16742..17911
                     /gene="prpC"
                     /locus_tag="ACHKUC_000711"
     CDS             16742..17911
                     /gene="prpC"
                     /locus_tag="ACHKUC_000711"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO6617012.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            17945..19396
                     /gene="prpD"
                     /locus_tag="ACHKUC_000712"
     CDS             17945..19396
                     /gene="prpD"
                     /locus_tag="ACHKUC_000712"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO6617013.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYETAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNSADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            19436..21322
                     /gene="prpE"
                     /locus_tag="ACHKUC_000713"
     CDS             19436..21322
                     /gene="prpE"
                     /locus_tag="ACHKUC_000713"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO6617014.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALAMIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            21647..22906
                     /gene="codB"
                     /locus_tag="ACHKUC_000714"
     CDS             21647..22906
                     /gene="codB"
                     /locus_tag="ACHKUC_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6617015.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            22896..24179
                     /gene="codA"
                     /locus_tag="ACHKUC_000715"
     CDS             22896..24179
                     /gene="codA"
                     /locus_tag="ACHKUC_000715"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO6617016.1"
                     /translation="MSNNALQAIINARLPGKEGLWQIHLHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLETGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(24300..24911)
                     /gene="lacA"
                     /locus_tag="ACHKUC_000716"
     CDS             complement(24300..24911)
                     /gene="lacA"
                     /locus_tag="ACHKUC_000716"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO6617017.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRIIREINDRDKQYYFKDFKVESSV"
     gene            complement(24977..26230)
                     /gene="lacY"
                     /locus_tag="ACHKUC_000717"
     CDS             complement(24977..26230)
                     /gene="lacY"
                     /locus_tag="ACHKUC_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO6617018.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATVEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(26282..29356)
                     /gene="lacZ"
                     /locus_tag="ACHKUC_000718"
     CDS             complement(26282..29356)
                     /gene="lacZ"
                     /locus_tag="ACHKUC_000718"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO6617019.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLERDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLHAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQSEAALLQCTADTLADVVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(29479..30561)
                     /gene="lacI"
                     /locus_tag="ACHKUC_000719"
     CDS             complement(29479..30561)
                     /gene="lacI"
                     /locus_tag="ACHKUC_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO6617020.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSWLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(30598..30696)
                     /locus_tag="ACHKUC_000720"
                     /pseudo
     CDS             complement(30598..30696)
                     /locus_tag="ACHKUC_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
     gene            30763..31302
                     /gene="yaiL"
                     /locus_tag="ACHKUC_000721"
     CDS             30763..31302
                     /gene="yaiL"
                     /locus_tag="ACHKUC_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO6617021.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNNEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(31528..32361)
                     /gene="fghA"
                     /locus_tag="ACHKUC_000722"
     CDS             complement(31528..32361)
                     /gene="fghA"
                     /locus_tag="ACHKUC_000722"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6617022.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(32454..33563)
                     /gene="frmA"
                     /locus_tag="ACHKUC_000723"
     CDS             complement(32454..33563)
                     /gene="frmA"
                     /locus_tag="ACHKUC_000723"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO6617023.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(33598..33873)
                     /gene="frmR"
                     /locus_tag="ACHKUC_000724"
     CDS             complement(33598..33873)
                     /gene="frmR"
                     /locus_tag="ACHKUC_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO6617024.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(34062..34835)
                     /gene="yaiO"
                     /locus_tag="ACHKUC_000725"
     CDS             complement(34062..34835)
                     /gene="yaiO"
                     /locus_tag="ACHKUC_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6617025.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENTTLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVISSYRYTH
                     YDSSDAGGSYSNMISVRLNNPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(34837..>35151)
                     /locus_tag="ACHKUC_000726"
     CDS             complement(34837..>35151)
                     /locus_tag="ACHKUC_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6617026.1"
                     /translation="IGPQCFIADSVVANQAYLGAQVRTSNHRLDEQPVSVRTPEGIIA
                     TGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPTGTYSLRQELIRT
                     GD"
BASE COUNT         8655 a         8979 c         9347 g         8170 t
ORIGIN      
        1 attcagtctt aaaaagcagg ttgttaaggg ataggcatag agtaatgacg tgtatgcata
       61 accaacatct ttactcatta tgtcattgaa tgttgacgct atgtgtttat gaggttgagg
      121 tattttcagt tgatctggat tgttaaattc atataatgcg cctttactca tgaatgggcg
      181 tcaatacgta gtgggaaatt ataaatatcg aaatagtcca actacttctt tattaccaaa
      241 aatgagtatc tgaattttaa tattgcattc ttgcgtgatt atctcctgag tttgacttgt
      301 gattaccttt ttaaggtatt tagcgtaact gtttttgagc gagcatcaga ggtaaagata
      361 atcttcttga tagtgatgtg ggatgttata cgtatggcat cgctaatgtt tatggttacc
      421 ccttatgtgt gctcaggaat ccacaggtaa tcactcatac tgaacagcga taaaagataa
      481 aaggtgtgtt catgaattca tgtgattttc gtgtttttct gcaagagttc ggtacaacgg
      541 ttcatttgtc attgcctggt agcgtatccg agaaagaacg actgctactc aagctgctga
      601 tgcagggaat gtctgtaaca gaaatatcac agtacagaaa tcgcagtgca aagaccattt
      661 cacatcaaaa gaaacagctc tttgagaaac tggggattca gagcgatatt actttctggc
      721 gggatatttt ctttcagtac aatccggaga tcatatccgc tacgggtaat aatagtcaca
      781 aatatattaa tgataatcac tatcaccata tcgtcacgcc tgaagccatc agtctggcgt
      841 tggaaaacca cgagtttaaa ccgtggatcc aaccggtttt ctgcgcgcag actggggtac
      901 tgacaggctg tgaagtgctt gtccgctggg aacatccaca aacgggaatg atcccaccgg
      961 atcagtttat tcctctggcg gagtcatccg gtcttattgt cataatgact cgccagctga
     1021 tgaaacagac tgcggatatt ctgatgccgg taaaacattt gctgccggac aatttccata
     1081 ttggtatcaa cgtctcggcg ggctgttttt tggctgcggg atttgaaaaa gagtgtctga
     1141 acctggttaa gaaattaggt aacgataaaa tcaagctggt tcttgagctg acggaacgta
     1201 atcctattcc ggtaacgcca gaagccagag cgatatttga cagccttcat cagcacaata
     1261 ttacctttgc gctggatgac tttggtacgg gttatgcgac ctatcgttac ttgcaggcgt
     1321 tcccggtcga ttttattaaa attgacaagt cgtttgtgca aatggcgagt gtcgacgaaa
     1381 tctccggtca tattgtggac aatattgtcg aactggcgcg taagcctggt ttgagtatcg
     1441 tggcggaagg ggtagaaacc caggagcagg cggatttaat gatcggtaaa ggcgttcact
     1501 ttttacaggg ctatttgtac tctccgccag taccgggtaa taaatttatc tctgaatggg
     1561 taatgaaagc aggtggttga tgtaaaccgc tattcacagc gcatcgggag gttggcagcg
     1621 attaattctc ccgatgcagt aaatcctgat agatccccgt taatatgcca tttggcgcaa
     1681 attgtttttt aatccactgc gtcacctgac cggtggcaga gtgctgggtt gctagtaaca
     1741 tgcgtgaatc ctggcgcggg ttatggattt gtcgggtaat cagcaaggat tgcgccatcg
     1801 cctcacggac catgtaatcc ggcaaaaaac caattccttc acctaaaatc tggcactgac
     1861 atttggtgtt gaaatctggc accagaattg actcctgccc atgcagcaac cagcccactt
     1921 ttttattaat cgtatgcgcg gtgtcctcca ccatgatatt agggtagaga cgcagctggc
     1981 tttcggcgat cggctccggc acaaaggcta atgggtgatc cggggcgatg gcaaaagccc
     2041 agcgaatcgc gccaatctcc gtgtaatcaa tgccgccgcc gtccagcagc gtgtcgggcg
     2101 cgccgatggc gatatttgcc tgattattaa taatcgcatc ccagacgccg ttatacactt
     2161 cggtcgtcac ggtaatctgg caggtaggaa actgcttttt cagcacctgt aataagcgcg
     2221 cggtgtgttt gggcgtataa agcagctgat taatacaaat gcgcacacgc gcttctatgc
     2281 cctgcgaaat agtatcgata ctgcgtttaa tggcgtgaaa atcattcagc aggtcggtag
     2341 ctttgcggaa aaaataacgc ccggactccg ttaactcaat gctgcgggta ctgcgagtga
     2401 acagcaccac atccagcccc gtctccatac gtttaatggt gtagctaatg gcggaagtgg
     2461 ttaaacccaa ctcttctgcg gctttactga agctgccaaa acgcgcagcg gtagtaaatg
     2521 ccaacagatt ctcttcggta aaaattgagt tcattaaacc attcccgcca ttatctaata
     2581 tgaatttctc caggcataac tgtagaggta tcaattaaag atgggtaatc atttttgaat
     2641 aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc gccatattct
     2701 gaaattatcc ccacgcagtt acccgccaga aaacccagta atgtcaccgt ccagttcatc
     2761 ccgctggcaa aaaatagcgt catgccgaga aagcccccgg gaataaaaga cagtaaccag
     2821 aaacgccctt gccagaccac caccgccgag aagggtacag tcgtaattac gctcgcccag
     2881 accccggaaa tcccactcac cggggccagc caaccggcag ccagcgcggc gaaaaacgcc
     2941 cacaccatcc cggaataatt aaccgccagg cttctgataa accccgattt accgccgcct
     3001 gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc ccatccctga
     3061 gagagcccga catgtgaaga cactaactgc caaagccctg cacaaatacc gacggtgaca
     3121 cccgttgccg tgagtccgtt catcttgttc ccctctggtt agacatggac gaaagctact
     3181 cttacatgtg cagactatag agaaataaaa cctgattgag tgaaagggta tcgggtcaaa
     3241 gaaacaaata ttgcactacc gcacacggcg aaaagattgt tgaacatcat tcaacaaaaa
     3301 ggcgaaatac ggcgaattaa atttaagcgg gttcttttat cagattatcg ttgctattct
     3361 caattcatcg aaaacagcca caaaaaccag gtgaatgtga atgactataa aaaatctacc
     3421 tgcagactat ttattggctg cgcaacaggg tgatatcgat aaagtaaaaa cctgccttgc
     3481 gctgggtgtc gatattaata cctgcgatcg tcaggggaaa acggcaatta cgctggcaag
     3541 tttatatcag caatatgctt gcgttcaggc attaattgac gccggagcga atattaataa
     3601 acaagatcat acctgtttaa atcctttttt aatgagctgt ctgaacgatg atttaacgct
     3661 actacgaatt attttaccgg ctaaacccga tcttaattgc gtaacccgtt ttggcggtgt
     3721 cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt gtaaaagagc ttttggcaaa
     3781 tacggagatt aacgttaacc agactaacca tgttggctgg acaccgttgc tggaagcgat
     3841 tgtgcttaac gatggcggga ttaaacagca ggcgattgtc cagttattgc tggaacacgg
     3901 tgccagcccg catctgaccg ataaatatgg caaaacgccg ctggaactgg cgcgggaacg
     3961 gggctttgaa gagattgcgc agttgctgat cgccgcaggt gcataaaccg ggaggcttgt
     4021 tatcaacaca ccagaaaaac ggtgtgtgtg ggcgctaact gcggatgctg attttctggc
     4081 gcagcggggg caaggacagg ttgaacaggt ctttgccaga gcggtaaata tcgtactccc
     4141 ggctcgccag cagttgctga cgctgctttg tgaagagtac gacaatgcgc ctaacagttg
     4201 tcggttggca ctcactcact ttaatggtct gttccggcat ggtgataagg ttcagtttga
     4261 cgatcaaggc attacgattg gtcaacatca tcatatagag atgagtcatt gccagcgttg
     4321 gctgtcccca accatgcaaa tgaccgctct gaattttcac cttatcgcct ggcaacagtg
     4381 gcacgacatt attcatcagc acctggggga aaatgaaacc ctgtttaatt atcgcggcga
     4441 taatccgttt tatcaggcgt taaataaaga actacatatt aaacgtcggg cagttattca
     4501 ggccgtaaac gataaacaaa atatcgccgt agcggtcgcc agtatgatgg gcttagggat
     4561 tggccttacg ccatcagccg acgattattt aacaggtctg gcgcttattt tatttcttcc
     4621 cggacatccg gcggaaaaat acaaagagga attttatctc ggtctgcaac gcggcagaaa
     4681 taataccacg ttattaagtg ccatcacgct ggaagccgca ttacaacaac gctgccggga
     4741 aaatattcat cgttttattc acaacattat ttatgacatc cctgggaacg caactcaggc
     4801 aatagaaaaa attaaacata ttggctccag ttccggctgc gacatgctgt atggcatggc
     4861 cgatggttgt gcgctgagtc aaacttacgg agggaattat gtcagttaaa atagtcatta
     4921 aaccgaatac ctattttgat tctgtctcgc tgatgtctat ctccacgcgt gcaaataaac
     4981 tcgacggcgt cgagcaggca tttgtggcga tggcgaccga aatgaacaaa ggcgtgttga
     5041 agaatttagg actgctgacg ccggagctgg agcaggcgaa aaacggcgac ctgatgattg
     5101 tcatcaatgg taaatcgggt gtggacaacg agcagttgct ggcggatatt gaagaactgt
     5161 tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta cgccactatt gccagcgcca
     5221 aaaagcatat cccggaaagt aacctggcag tgatttcggt caacggtctg tttgccgctc
     5281 gcgaagcgcg tcaggcgctg caaaatgacc tcaacgtgat gctgttttcc gataatgtct
     5341 cagctgaaga tgaactggcg ctcaaacagt tggcccatga aaaagggctg ctgatgatgg
     5401 ggccagactg cggcacggcg attatcaacg gcgcggcact ctgctttggt aacgccgtgc
     5461 gtcgcggcaa catcggtatt gttggcgcat ctggcaccgg cagccaggaa ttgagcgtcc
     5521 gtatccatga atttggtggc ggcgtttcac aactgatcgg caccggcggg cgcgacctga
     5581 gcgagaaaat cggtggcctg atgatgctcg atgccatcgg gatgctggaa aacgatccgc
     5641 aaactgaaat cattgtgctt atctccaaac cgcctgcgcc tgcggtggct cgcaaagtgc
     5701 tggaacgcgc acgcgcctgc cgcaagccag tggtggcctg cttcctcggt cgtggcgaaa
     5761 cgtcagtgga tgagcagggg ctacagtttg ctcgcggcag caaagaggcg gcattgaaag
     5821 cggtgatgct ctccggcgtg aaacaagaac atctcgacct gcatacgctt aaccagccgt
     5881 tgattgcgga tgtgcgggcg cgtctgcaac cgcagcagaa atacattcgt ggtctgttct
     5941 gcggcggtac gctgtgtgac gaaaccctgt tcgcggtgat ggaaaaacat ggcgatgtct
     6001 acagcaatat ccagcccgat cctgaattcc gcctgcaaga tatcaaccgc agcatcaaac
     6061 acaccttcct cgattttggc gatgatgact tcaccaacgg caagccgcat ccaatgattg
     6121 acccgaccaa ccgtatcagc cgcttgatcg aagaagcgcg cgatccagaa gtggcggtga
     6181 tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga tccggtcggt tccaccatcg
     6241 aggcgatcaa agaagcgaaa gcgatcgccg ctgccgaagg acgcgagttg atcattctcg
     6301 cctatgtgct gggcaccgat ctcgatacgc catcattaga acaacaaagc cagatgctgc
     6361 ttgatgccgg agtgattctg gcgagcagca gcaccaatac cggattgctg gcgcgtgaat
     6421 ttatctgcaa aggggaggaa gcctgatgag ccagtcactg tttagccaac cgttgaacgt
     6481 tattaacgtc ggtatcgcca tgtttagcga tgatctgaaa aagcagcatg tagaagtgac
     6541 ccaactcgac tggacgccgc ggggcagggc aatatgcagg tggtgcaggc gctggataac
     6601 attgccgatt cgccgctggc agacaaaatc gccgccgcta accagcaggc gctggagcgc
     6661 attatccagt cgcatccggt gctgattggt tttgatcagg cgattaatgt ggtgccgggc
     6721 atgacggcga aaaccattct tcacgccggg ccgccggtta cctgggaaaa aatgtgcggc
     6781 gcgatgaaag gcgcggtcac cggggcgctg gtgttcgaag gactggcgaa agatctcgac
     6841 gaggcggctg aactggcggc ttccggggag atcaccttct cgccgtgcca cgagcatgac
     6901 tgcgtgggat cgatggcggg cgtcacctcg gcctcgatgt ttatgcacat cgtgaaaaac
     6961 aaaacctatg gcaacatcgc ttataccaac atgagcgagc agatggcgaa gattttgcgt
     7021 atgggcgcta acgaccagag cgtgatcgac cgcctcaact ggatgcgcga tgtgcaggga
     7081 cccatgctgc gcgacgcgat gaaaattatc ggcgaaatcg atctgcgctt aatgctggcg
     7141 caggcgctgc atatgggcga tgaatgccat aaccgcaata acgccgggac gacgctgctg
     7201 attcaggcgc tgacgccggg gattattcag gcaggttatt ccgtcgagca acagcgcgaa
     7261 gtgtttgagt ttgtcgccag cagcgactac ttctccggcc cgacgtggat ggcgatgtgt
     7321 aaagcggcga tggatgcggc gcacggcatc gaatacagca ccgtggtgac caccatggcg
     7381 cgtaacggcg tcgagttcgg cctgcgggtc agcggcctgc cggggcagtg gtttaccggc
     7441 ccggcgcagc aggtcatcgg cccgatgttt gccggttata agccggaaga ttcagggctg
     7501 gatattggcg acagcgccat caccgaaacc tacggtattg gcggatttgc gatggcgacc
     7561 gcgcccgcga tcgtcgcgct ggtggggggc acggtggaag aggctatcga tttctcccgt
     7621 cagatgcgtg aaatcaccct tggcgaaaac cccaacgtca ccattccgct gctcggtttt
     7681 atgggcgtgc cgtcggcaat cgacatcacc cgcgtgggta gcagcggcat tctgccagtg
     7741 atcaacaccg ccatcgccca taaagatgcg ggcgtcggca tgattggcgc gggcattgtg
     7801 catccaccct tcgcctgctt cgagaaagcc atttttggct ggtgcgaacg ttacggcgtc
     7861 tgatctgtaa agtgcgcgtt gtttctgccg gatgcggcgt gaacgcctta tccggcctac
     7921 gagtggcgca agaatctgta ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt
     7981 cataacccca tcaccctctg tcgcggacat aacaacatga aagaacttgt ggtcgttgcc
     8041 attggtggca acagcattat caaagataac gccagccagt cgattgagca tcaggcggag
     8101 gcggtgaaag ccgtggccga tacggtgctg gaaatgctgg cttccgatta cgacattgtg
     8161 ctgacccacg gcaacgggcc gcaggtcggg ctggatttac gccgcgcgga gattgcccac
     8221 gagcgcgaag ggctgccctt aacgccgctg gcgaactgtg tggcggatac gcaaggtggc
     8281 attggctatc tgatccagca ggcgctgaac aaccgactgg cgcgtcacgg cgagaaaaaa
     8341 gccgtcaccg tggtgactca ggtggaagtg gataaaaacg atccggggtt tgcccatccc
     8401 accaagccca tcggcgaatt ctttagtgaa agccagcgtg acgaactaca aaaggcaaac
     8461 cctgactggc gttttgttga agatgccgga cggggctatc gccgcgtggt cgcctcgccg
     8521 gaaccgaaac gtattgtcga agcacctgcc attaaggcgc tgatccaaca aggctttgtg
     8581 gtgattggcg cgggcggcgg tggaattcct gtggtgcgca ccgaagcggg agattaccaa
     8641 agcgtggacg cggttatcga caaagatctc tctaccgcgc tactggctcg cgaaattcac
     8701 gccgacattc tggtgatcac cactggcgtc gaaaaagtgt gtattcactt tggcaaaccg
     8761 cagcagcagg cgctggatcg cgtagatatt gccaccatga cccgctatat gcaggaaggg
     8821 catttcccac ctggcagcat gttgccaaaa atcatcgcca gcctgacgtt cctggaacaa
     8881 ggcggcaaag aagtgattat caccacgccg gaatgcctgc ctgcggcgct acgcggcgaa
     8941 acgggcaccc atattattaa aacctaagga cgtaagatga aagaaaacaa tagccgccgt
     9001 gaatttctga gccagagcgg taagatggtt accgccgccg cgctgtttgg tccctcagtg
     9061 ccgctcgccc atgcgggggg agctggcacc ctaaactgcg aagcgaacaa caccatgaaa
     9121 atcactgacc cgcattacta tctcgataac gtgctgctgg aaaccggttt tgactacgaa
     9181 aatggcgtgg cggtgcagac ccgcacggcg cgccagactg tggaaattca gaacggtaaa
     9241 attgttgccc tgcgcgagaa caagcagcat ccggacgcca cgctgccgca ctatgacgct
     9301 ggcggtaagc tgatgctgcc caccacccgc gacatgcata ttcatctcga caaaaccttc
     9361 tacggcgggc cgtggcgctc gctcaatcgt ccggcaggca ccactatcca ggacatgatc
     9421 aaactcgagc agaaaatgct gccggaactg caaccgtaca cgcaggaacg ggcggaaaaa
     9481 ctgatcgatt tattgcagtc gaaaggcacc accattgccc gcagccactg caatatcgaa
     9541 ccggtttccg gcctgaaaaa tctgcaaaat ttgcaggcgg tgctggcgcg acgtcaggcg
     9601 ggcttcaagt gtgaaatcgt cgcctttccg cagcacggtt tgctgctgtc gaaatcggaa
     9661 tccttaatgc gcgaagcgat gcaggcgggg gcgcattacg tcggcgggct ggacccgacc
     9721 agtgttgatg gcgcgatgga aaaatccctc gacaccatgt tccagattgc gctggactat
     9781 gacaaaggcg tcgatattca cctgcacgaa accacgccgg caggcgtggc agccatcaat
     9841 tatatggttg aaacagtgga gaaaacgcca cagctgaagg gcaagctgac catcagccac
     9901 gcctttgcgc tggcaacgct caacgaacaa caggtagatg aactggcgca ccggatggcg
     9961 gcgcagcgaa tttcgatcgc ttcgacggtg ccgattggca cgctgcatat gccgctcaaa
    10021 cagctgcacg acaaaggcgt aaaagtgatg accggcaccg acagcgttat cgaccactgg
    10081 tcgccttacg gcctgggcga catgctggaa aaagccaatc tgtacgcgca gctttatatt
    10141 cgtcctaacg aacagaacct ctcccgctcg ctgtttttag ccactggcga tatattgccg
    10201 ctgaatgaaa aaggcgagcg tgtgtggcct aaagcgcagg atgacgccag ttttgtgctg
    10261 gtggacgcct cctgttccgc cgaggcggtg gcgcgtattt cgccgagaac ggcgacgttc
    10321 cataaagggc aactggtgtg gggaagtgtg gcaggttgat acgcatttga aacgcctgca
    10381 acggtgaatc gtaagagatt taaagcccca gggagtgggg cggcctgagt atgagtgtgt
    10441 tggcagaaat ggacattatc tttgaagaat atgcccttat tgacgttgtg tagataacta
    10501 tttgtctacg tgaaaatatc atcagttttg ccgatttttg tcttttacgg atagcaaata
    10561 tcacacttat aggcgctcgc cttatgggga acgttgctgc tctgtttcat tcaggctcgc
    10621 ggcatgttgg ggctggatcg ggtggtttaa atattggcta tattcaatgg acgcgttttg
    10681 ccgcgatgac atatcaggca tcgccaaata cacatagcta atcaggagta aacacaatga
    10741 agatcaaagc tgttggtgca tattccgcta aacaaccgct tgaaccgatg gatatcaccc
    10801 ggcgtgaacc gggaccgcat gatgtcaaaa tcgaaatcgc ttactgtggc gtctgccatt
    10861 ccgatctcca ccaggtccgt tcggagtggg cggggacggt ttacccctgc gtgccgggtc
    10921 atgaaattgt ggggcgcgtg gtagccgttg gtgatcaggt agaaaaacat gcgccgggcg
    10981 atctggtcgg tgtcggctgc attgtcgaca gttgtaaaca ttgcgaagag tgtgaagacg
    11041 ggctggaaaa ctactgtgat cacatgaccg gcacctataa ctcgccgaca ccggatgaac
    11101 cgggccatac tctgggcggc tactcacaac agatcgtcgt tcatgagcga tatgttctgc
    11161 gtattcgtca cccgcaagag cagttggcgg cggtggctcc tttattgtgt gcaggaatca
    11221 ccacgtactc gccgctacgc cactggcagg ccgggccggg taaaaaagtg ggcgtggtcg
    11281 gcatcggcgg tctgggacat atggggatta aactggccca cgcgatgggg gggcgcatgt
    11341 ggtggcattt accacttctg agtcaaaacg cgaagcggca aaagccctgg gggccgatga
    11401 agttgttaac tcacgcaatg ccgatgagat ggtggctcat gtgaagagtt tcgatttcat
    11461 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgcga
    11521 tggcaccata acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
    11581 cctgatcatg aaacgccgcg cgatagctgg ttctatgatt ggcggcattc cagaaactca
    11641 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
    11701 cgatcgaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
    11761 tatcgataat cgcacactaa cagactgaaa aaattaataa acatcctgtg gtttaacata
    11821 ttaacttcgc tccacttaac tttttagtta aggagagcga aataatatca aagtagtggt
    11881 aaacctatag cgtaaattca gattgttaaa ttaactccac tactttacac gttagttatg
    11941 agcgatttct gatagtgtct ggtttaatca gagctttatt atctgcgacg tttattttta
    12001 tttaagagag ccacctgcaa gttgttattc caccagagag tgaatcgcaa agcaggtcat
    12061 catttcatct gtatatctgt atgccaaatg gcattccccc tccttttccg ccgatatttt
    12121 gttgtgaacg gcggagtttc gcttaaaaaa gcaccagcag tggttttaca gctatatggt
    12181 gtataaaaaa tgttctcata cagatgtttt gtgaatgtgt gggttgacat tcatatgaaa
    12241 aaaatcataa ttccatcatg tttgtgtggg gtcttttctg tatcttacgc atcgcactca
    12301 agcctgacag aaaatatgtt gtaaggctca tatcaaaaac cgccattagc tcatcaggaa
    12361 gagcagacga caaccaggat tgttgtatgg tacggggttc gaggcctcga tggcggtaca
    12421 acttttcaaa acttttctga atcagatccc cttattatca tccctgcaaa gtggaacatt
    12481 gccattgttt ggctattcag gacgggtcag accaatgaaa aagcccacac aggggagagt
    12541 ggctgaaatg ggaagctaaa gactcaagta aacttatcgg aaataaggac cacgcattac
    12601 gggggctatc atcgccgatg gttgggattc ggcaaatcag gttgattacg tagagagtaa
    12661 attatctgct caccgctgcg tcaccccttc gtaaatcagg cgtagcgcga atacaccaat
    12721 aattgcacca ataacccaac tggcaacgcg ttgcatacgc ccataagcac gacgcactgc
    12781 gggcaaagaa aacgcctgac taagaaagac tcgccagata attgatgcga gcacaatccc
    12841 cgcccaggcc attaatcgtg cccatgttgg tgtttcggca tgtaatgtta ctgagaaaat
    12901 actgataaaa aataaaacgg tttgcgggtt agagagatcg gtaattaatc cacggcggaa
    12961 aaaaacatac cagggggcgc taatcggttg ttgtagtgtg ctcatttgcg gtgttgagtg
    13021 gcggcgcatg ctactacacc acgcaaacca taagagataa gcgccgccga cgattctgat
    13081 aagcgaaaaa atctccccac actgcgtaat tagcgttgca agaccaaaca aacccaaccc
    13141 ggaataaaat gcatcgccca gcgccacgcc cagcccggtc aacacccctg cgcgtcgacc
    13201 ggaagccagg ctggtttgta ctaccacaaa gagattggct cccggattaa aaaaagtaat
    13261 cacgaacaat cctacggtca ggtaaacggc gtgcaaagga tccatagtga tttcatccat
    13321 aaataagtga agtaacttca tcatatttct accaaaaata atcgggtgcg agagagatca
    13381 caaagtgtct tatttccggt tactggcgtt tatgccctga ctgaactaat tattaatcaa
    13441 cccaataatg tgggtgggtg atagtgtgat aacaactctg gagccgtaat atgaaaataa
    13501 tctctaaaat gttagtcggt gcgttagcgt ttgccgttac caatgtctat gccgcagaat
    13561 taatgaccaa agcggaattt gaaaaggttg catcgcaata tgaaaaaata ggtgatattt
    13621 caaccagtaa tgaaatgtcg accgcagatg cgaaagaaga tttgatcaaa aaagccgatg
    13681 aaaaaggggc tgatgtgttg gtactgacct ctggtcaaac tgacaataag atccacggca
    13741 cggcaaatat ttataagaag aagtaattct gaatcctatg taaacatccc cgatgcgtaa
    13801 gtttatcggt gatcatcatc tattgaaatt tatgccggat aaagcgttcg cgctgcattc
    13861 ggcagttcag cttttcagcc gccgccagaa cgtcgttcgg ctgataccta aataattcgc
    13921 cgctgctgtc ttatcgccat taaatttctc cagtgcctgt tgtgctgtca gcaagcctgg
    13981 aatgggagtc ttcgccgact cgcgtgccag ttccggcagt agcagctgca aaaattgcgg
    14041 cgttaaatcc ggcgtcgatt ccacacttaa aaacagcgcc agtcgttcca tcatattgcg
    14101 cagttcacga atattgcccg gccagtcgta gtgcaccagc atggtttcgc ttgcctgtaa
    14161 cccctggcgt aatgcggcag caaacggggc ggagagcgcc gccagagaca ctttcaaaaa
    14221 gctttccgcc agcggcagaa tatccgccac ccgctcgcgt agcggcggca gttgcaggcg
    14281 taaaatactc agccgataaa acaggtcacg gcgaaactgc ccttgctgca tatcttcttc
    14341 cagattgcag tgagtggcgc taatgacccg cacatccacc ggaacaggct gatgcccgcc
    14401 gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgagtctgca acggcagcgg
    14461 catttcgcct atctcgtcaa gaaacagcgt gccaccgtgg gcgatttcaa acagcccggc
    14521 gcgtcctccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gctccgcttc
    14581 cagcagcgat tcggcaatcg ccccgcagtt aacggcaaca aacggatgtg actttttgcc
    14641 ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt
    14701 gcccgtcccc gtttcccctt caatcaacac cgccgcactg gagcgggcat acagcaaaat
    14761 agtctgccgc acctgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg
    14821 agtacgcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat
    14881 atccagcgca tcgctgaacg cctggcgtac ggtggcggcg gaataaataa aaattccggt
    14941 cattccggct tcttctgcca gatcggtaat cagccccgcg ccgaccaccg cttcggtgcc
    15001 gttagctttc agctcgttaa tctgcccgcg cgcgtcttct tcggtaatgt agctacgttg
    15061 gtcgaggcgc aaattaaagg ttttttgaaa cgccaccagc gccggaatag tttcctgata
    15121 agtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc
    15181 gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcacc
    15241 gttagagcca gcggcaatga tggcgtcaca gcgttcgctt gccagtttct tgcggatgta
    15301 ggtcaccgct ttttcaaagc caagctggat aggggtgata ttcgccaggt gatcaaactc
    15361 gaggctgata tcgcgaaata actcgaacag ccgcgttaca gataccgtcc agataaccgg
    15421 tttgtcgtca ttcagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa
    15481 tattgggttt agtcttgttt cataattgtt gcaatgaaac gcagtgaaac attgcttgaa
    15541 acgttaactg aaacgcatat ttgcggatta cttcatgact ttatttataa caaattgaaa
    15601 ttaaacattt aattttattc agtcaattgt ggcacacccc ttgccttgtc tttatcaacg
    15661 caaataacaa gttgataaca aaggatgggc tatgcctcta cactctccag gtaaagcgtt
    15721 tcgcgctgca cttagcaaag aaaccccgtt gcaaattgtt ggcaccatca acgctaacca
    15781 tgcgctgctg gcgcagcgtg ccggatatca ggcgatttat ctctccggcg gtggcgtggc
    15841 ggcaggatcg ctggggctgc ccgatctcgg tatttctact cttgatgatg tgctgactga
    15901 cattcgccgt attaccgacg tttgttcgct gccgctgctg gtggatgcgg atatcggttt
    15961 tggttcttca gcctttaacg tggcgcgtac ggtgaaatca atgattaaag ccggtgcggc
    16021 aggattgcat attgaagatc aggttggtgc gaaacgctgc ggtcatcgtc cgaataaagc
    16081 gatcgtctcg aaagaagaga tggtggatcg gatccgcgcg gcggtggatg cgaaaaccga
    16141 tcctgatttt gtgatcatgg cgcgcaccga tgcgctggcg gtagaggggc tggatgcggc
    16201 aatcgaacga gcggaggcct atgttgaagc gggtgccgag atgctgttcc cggaagcaat
    16261 taccgaactc gccatgtacc gccagtttgc cgatgcggtg caggtgccga tccttgccaa
    16321 cattactgaa tttggtgcca cgccgctgtt taccaccgac gaattacgca gcgcccatgt
    16381 cgcaatggcg ctgtatcccc tttctgcttt ccgcgccatg aaccgcgccg ctgaacatgt
    16441 ctacaacgtc ctgcgccagg aaggcacgca gaaaagcgtc atcgacacca tgcaaacccg
    16501 caacgagctg tacgaaagca tcaactacta ccagtacgaa gagaagctcg acgacctgtt
    16561 tgcccgtggt caggtgaaat aaaaacgccc gttggttgta ttcgacaacc gatgcctgat
    16621 gcgccgctga cgcgacttat caggcctacg aggtgaactg aactgtaggt cggataagac
    16681 gcatagcgtc gcatccgaca acaatctcga ccctacaaat gataacaatg acgaggacaa
    16741 tatgagcgac acaacgatcc tgcaaaacag tacccatgtc attaaaccga aaaaatcggt
    16801 ggcactttcc ggcgttccgg cgggcaatac ggcactctgc accgtgggta aaagcggcaa
    16861 cgacctgcat taccgtggct acgatattct tgatctggcg gaacattgtg aatttgaaga
    16921 agtggcgcac ctgctgatcc acggcaaact gccaacccgt gacgaactcg ccgcctacaa
    16981 aacgaaactg aaagccctgc gtggtttacc ggctaacgtg cgtaccgtgc tggaagcctt
    17041 accggcggcg tcacacccga tggatgttat gcgcaccggc gtttccgcgc tcggctgcac
    17101 gctgccagaa aaagaggggc acactgtttc tggtgcgcgg gatattgccg acaaactgct
    17161 ggcgtcactt agttcgattc ttctctactg gtatcactac agccacaacg gcgaacgcat
    17221 ccagccggaa actgatgacg actctatcgg cggtcacttc ctgcatctgc tgcacggcga
    17281 aaagccgtcg caaagctggg aaaaggcgat gcatatctcg ctggtgctgt acgccgaaca
    17341 cgagtttaac gcttccacct ttaccagccg ggtgattgcg ggcactggct ctgatatgta
    17401 ttccgccatt attggcgcga ttggcgcact gcgcgggccg aaacacggcg gggcgaatga
    17461 agtgtcgctg gagatccagc aacgctacga aacgccggac gaagccgaag ctgatatccg
    17521 taagcgggtg gaaaacaaag aagtggtcat tggttttggg catccggttt ataccatcgc
    17581 cgacccgcgc caccaggtga tcaaacgcgt ggcaaagcag ctctcgcagg aaggcggctc
    17641 gctgaagatg tacaacatcg ccgatcgcct ggaaacagtg atgtgggaga gcaaaaagat
    17701 gttccccaat ctcgactggt tctccgctgt ttcctacaac atgatgggtg ttcccaccga
    17761 gatgttcaca ccactgtttg ttatcgcccg cgtcactggc tgggcggcgc acattatcga
    17821 acaacgtcag gacaacaaaa ttatccgtcc ttccgccaat tatgttggac cggaagaccg
    17881 ccagtttgtc gcgctggata agcgccagta aacctctacg aataacaata aggaaacgta
    17941 cccaatgtca gctcaaatca acaacatccg cccggaattt gatcgtgaaa tcgttgatat
    18001 cgtcgattac gtcatgaact acgaaatcag ctccagagtg gcctacgaga ccgcacatta
    18061 ctgcctgctc gacacgctcg gctgcggtct tgaagctctc gaatacccgg cctgtaaaaa
    18121 actgctgggg ccaattgtcc ccggcactgt cgtacctaac ggcgtgcgcg tccccggaac
    18181 tcagttccag ctcgaccccg tccaggcggc atttaacatt ggcgcgatga tccgttggct
    18241 cgatttcaac gatacttggc tggcggcgga gtgggggcat ccttccgaca acctcggcgg
    18301 cattctggca acggcagact ggctttcgcg caacgcggtc gccagcggca aagcgccgtt
    18361 gaccatgaaa caggtgctga ccgcaatgat caaagcccat gaaattcagg gctgcatcgc
    18421 gctggaaaac tcctttaacc gcgtcggcct cgaccacgtt ctgttagtga aagtggcttc
    18481 caccgccgtg gtcgccgaaa tgctcggcct gacccgcgag gaaattctca acgccgtttc
    18541 gctggcatgg gtagacggac agtcgctgcg cacctatcgc catgcgccga acaccggtac
    18601 gcgtaaatcc tgggcggcgg gcgatgccac ttcccgcgcg gtacgtctgg cactgatggc
    18661 gaaaaccggc gaaatgggtt atccgtcagc cctgactgcg cctgtgtggg gcttctacga
    18721 cgtctccttt aaaggcgaat cgttccgctt ccagcgcccg tatggttctt acgtgatgga
    18781 aaatgtgctg ttcaaaatct ccttcccggc ggagttccac tcccagacgg cagttgaagc
    18841 agcgatgacg ctctatgaac agatgcaggc agcaggcaaa acggcggcgg atatcgaaaa
    18901 agtgaccatt cgcacccacg aagcctgtat tcgcatcatc gacaaaaaag ggccgctcaa
    18961 taactcggct gaccgcgacc actgcattca gtacatggtg gcgatcccgc tgctgttcgg
    19021 gcgcttaacg gcggcagatt acgaggacaa cgttgcgcaa gataaacgca tcgacgccct
    19081 gcgcgagaag atcaattgct ttgaagatcc ggtgtttacc gctgactacc acgacccgga
    19141 aaaacgcgcc atcgccaatg ccataaccct tgagttcacc gacggcacgc gatttgaaga
    19201 agtggtggtg gagtacccaa ttggtcatgc tcgccgccgt caggatggca ttccgaagct
    19261 ggtcgataaa ttcaaaatca atctcgcgcg ccagttcccg actcgccagc agcagcgcat
    19321 tctggaggtt tctctcgaca gaactcgcct ggaacagatg ccggtcaatg agtatctcga
    19381 cctgtacgtc atttaagtaa acggcggtaa ggcgtaagtt caacaggaga gcattatgtc
    19441 ttttagcgaa ttttatcagc gttcgattaa cgaaccggag cagttctggg ccgagcaggc
    19501 ccggcgtatt gactggcaga cgccctttac gcaaacgctc gatcacagca atccgccgtt
    19561 tgcccgttgg ttttgtgaag gccgaaccaa cttgtgtcac aacgctatcg accgctggct
    19621 ggagaaacag ccagaggcgc tggcgatgat tgccgtctct tcggaaacag aagaagagcg
    19681 cacctttacc tttcgccagc tgcatgacga agtgaatgcg gtggcgtcaa tgctgcgctc
    19741 actgggcgtg cagcgtggcg atcgggtgct ggtgtatatg ccgatgattg ccgaagcgca
    19801 tattaccctg ctggcctgcg cgcgcattgg cgctattcat tcggtggtgt ttgggggatt
    19861 tgcctcgcac agcgtggcgg cgcgaattga tgatgctaaa ccggtgctga ttgtctcggc
    19921 tgatgccgga gcgcgcggtg gcaaaatcat tccctataaa aaattactcg acgatgcgat
    19981 aagtcaggcg cagcatcagc cgcgccacgt tttactggtg gatcgcgggc tggcgaaaat
    20041 ggcgcgcgtt agcgggcggg atgtcgattt cgcgtcgttg cgccatcagc acatcggcgc
    20101 gcgggtgccg gtggcatggc tggaatccaa cgaaacctcc tgcattctct acacctccgg
    20161 cacgaccggc aaacctaaag gtgtgcagcg tgatgtcggc ggatatgcgg tggcgctggc
    20221 gacctcgatg gacaccattt ttggcggcaa agcgggcggc gtgttctttt gtgcttcgga
    20281 tatcggctgg gtggtagggc attcgtatat cgtttacgcg ccgctgctgg cggggatggc
    20341 gactatcgtt tacgaaggat tgccgacctg gccggactgc ggcgtgtggt ggaaaattgt
    20401 cgagaaatat caggttagcc gcatgttttc agcgccgacc gccattcgcg tgctgaaaaa
    20461 attccctacc gctgaaattc gcaaacacga tctttcgtcg ctggaagtgc tctatctggc
    20521 tggagaaccg ctggacgagc cgaccgccag ttgggtgagc aatacgctgg atgtgccggt
    20581 catcgacaac tactggcaga ccgaatccgg ctggccgatt atggcgattg ctcgcggtct
    20641 ggatgacagg ccgacgcgtc tgggaagccc cggcgtgccg atgtatggct ataacgtgca
    20701 gttgctcaat gaagtcaccg gcgaaccgtg tggcgtcaat gagaaaggga tgctggtagt
    20761 ggaggggcca ttgccgccag gctgtattca gaccatctgg ggcgacgacg accgctttgt
    20821 gaagacgtac tggtcgctgt tttcccgtcc ggtgtacgcc acttttgact ggggcatccg
    20881 cgatgctgac ggttatcact ttattctcgg gcgcactgac gatgtgatta acgttgccgg
    20941 acatcggctg ggtacgcgtg agattgaaga gagtatctcc agtcatccgg gcgttgccga
    21001 agtggcggtg gttggggtga aagatgcgct gaaagggcag gtggcggtgg cgtttgtcat
    21061 tccgaaagag agcgacagtc tggaagaccg tgaggtggcg cactcgcaag agaaggcgat
    21121 tatggcgctg gtggacagcc agattggcaa ctttggccgc ccggcgcacg tctggtttgt
    21181 ctcgcaattg ccaaaaacgc gatccggaaa aatgctgcgc cgcacgatcc aggcgatttg
    21241 cgaaggacgc gatcctgggg atctgacgac cattgatgat ccggcgtcgt tggatcagat
    21301 ccgccaggcg atggaagagt aggtcggata aggcgctcgc gccgcatccg acaccgtgcg
    21361 cagatgcctg atgcgacgct gacgcgtctt atcatgccta caggcctgaa ccgtaggtcg
    21421 gataaggcgc tcgcgccgca tccgacacca tgcgcagatg cctgatgcga cgctgacgcg
    21481 tcttatcatg cctacccact gtttttacac agataagttt cccccgcctt tttgtactca
    21541 ttcatataag aaatatatcc ccctacgaaa acgattgctt tttatcttca gatgaataga
    21601 atgcggcgga ttttttgggt ttcaaacagc aaaaaggggg aatttcgtgt cgcaagataa
    21661 caactttagc caggggccag tcccgcagtc ggcgcggaaa ggggtattgg cattgacgtt
    21721 cgtcatgctg ggattaacct tcttttccgc cagtatgtgg accggcggca ctctcggaac
    21781 cggtcttagc tatcatgatt tcttcctcgc agttctcatc ggtaatcttc tcctcggtat
    21841 ttacacttca tttcttggtt acattggcgc aaaaaccggc ctgaccaccc atcttcttgc
    21901 tcgcttctcg tttggtgtta aaggctcatg gttgccttca ctgctgctgg gcggcaccca
    21961 ggttggctgg tttggcgttg gcgtggcgat gtttgctatt ccggtgggca aggcaaccgg
    22021 gctggatatt aatttgctga ttgccgtctc cggtttactg atgaccgtca ccgtcttctt
    22081 tggcatttcg gcgctgacag ttctttcggt gattgcggtt ccggctatcg cctgccttgg
    22141 cggttattcc gtgtggttgg ccgttaacgg catgggcgga ctggacgcat taaaagcggt
    22201 cgttcccgca caaccgttag atttcaatgt cgcgctggcg ctggttgtgg ggtcattcat
    22261 cagtgcgggt acgctcaccg ctgactttgt ccggtttggt cgtaatgcca aactggcggt
    22321 gctggtggcg atggtggcct ttttcctcgg caactcgttg atgtttattt tcggtgcagc
    22381 gggcgcggcg gcactgggga tggcggatat ctctgatgtg atgattgctc agggcctgct
    22441 gctgcctgcg attgtggtgc tggggctgaa tatctggacc accaacgata acgcgctcta
    22501 tgcgtcgggt ttaggtttcg ccaacattac cggtatgtcg agcaaaaccc tttcggtgat
    22561 taacggtatt atcggtacgg tctgcgcatt atggctgtat aacaattttg tcggctggtt
    22621 gaccttcctt tcggcagcta ttcctccggt gggcggcgtg atcatcgctg attatctgat
    22681 gaaccgtcgc cgctatgagc actttgcgac cacgcgtatg atgagtgtca attgggtggc
    22741 gattctggct gtcgcgctgg ggattgccgc aggccactgg ttaccgggaa ttgttccggt
    22801 caacgcggta ttaggtggcg cgctgagcta tctgatcctt aacccgattt tgaatcgtaa
    22861 aacgacagca gcaatgacgc atgtggaggc taacagtgtc gaataatgct ttacaagcaa
    22921 ttattaacgc ccggttaccc ggcaaagagg ggctgtggca gattcatctt cacgacggaa
    22981 aaatcagcgc cattgatacg caatctggtg tgatgcccgt aactgaaaac agcctggatg
    23041 ccgaacaagg tttagtttta ccgccgtttg tggagccgca tattcacctg gacaccacgc
    23101 aaaccgccgg acaaccgaac tggaatcagt ccggcacgct gtttgaaggc attgaacgct
    23161 gggccgagcg caaagcgtta ttaactcatg acgatgtgaa acaacgcgca tggcaaacgc
    23221 tgaaatggca gattgccaac ggcattcagc atgtgcgtac ccatgtcgat gtttcggacg
    23281 caacgctgac tgcgctgaaa gcaatgctgg aagtgaaaca ggaagttgcg ccgtggattg
    23341 atctgcaaat cgtcgccttc ccgcaggaag ggattttgtc gtatcccaac ggtgaagcgt
    23401 tgctggaaga ggcgctacgc ttaggggcag atgttgttgg ggcgattccg cattttgaat
    23461 ttacccgtga atacggcgtg gagtcgctgc ataaaacctt cgccctggcg caaaaatacg
    23521 accgtctcat cgacgttcac tgtgatgaga tcgatgacga gcagtcgcgc tttgtcgaaa
    23581 ccgttgctgc cctggcgcac cgtgaaggca tgggcgcgcg agtcaccgcc agccatacta
    23641 cggcaatgca ctcctataac ggggcgtata cttcgcgcct gttccgtttg ctgaaaatgt
    23701 ccggtattaa ctttgtcgcc aacccgttgg tcaatattca tctgcaagga cgtttcgata
    23761 cgtatccaaa acgtcgcggc atcacgcgcg ttaaagagat gctggagacc ggcattaacg
    23821 tctgctttgg tcacgatgat gtcttcgatc cgtggtatcc gctgggaacg gcgaatatgc
    23881 tgcaagtgct gcatatgggg ctgcatgttt gccagctgat gggctacggg cagattaacg
    23941 acggcctgaa tttaatcacc caccacagcg ccaggacgtt gaatttgcag gattacggca
    24001 ttgccgccgg aaacagcgcc aacctgatta tcctgcctgc tgaaaatggg tttgatgcgt
    24061 tgcgccgtca ggttccggta cgttattcgg tacgtggcgg taaggtgatt gccagcacac
    24121 aaccggcaca aaccaccgta tatctggagc agccagaagc catcgattac aaacggtaat
    24181 cggctgggct accgcgagcg tagtttatgc cggatgcggc gtgaacacct tatccggcct
    24241 acgtagatca ctgaaattgt aggcctgata agcggagcgc atcaggtaat tttgcttatt
    24301 taaactgaag actcaacttt aaaatctttg aaataatatt gcttatcccg gtcgtttatt
    24361 tcgcgaataa tccgacaagg aacgccagca gccacgacgt ttggtggaat gtcttttgtg
    24421 acgacactac ccgcgccaat aacagaatta tccccgatgg tgacgcctgg attaataacc
    24481 acatgacttc cgatccagac gttattgcca atcgttatcg gaaaagagta catttctccg
    24541 ttttttctca attcatggtg taccgggtgt cccgtaacgg aaagagtaac gttgggtgca
    24601 atcagtacgt tatcaccgat cgttaccgtg tagtcgtcga caatggttaa attgaaattt
    24661 gcataaaaat tgcggcctat atggatgttg gaaccgtaag agaaatagac gggggggtct
    24721 acccaggcgt tttcccctac cgtggcaaac atttctttaa tcaggctttc tcttttatca
    24781 acttctgatg gatgcgagtg attaaactca tacattaacg tttttccacg aagtcttttt
    24841 tcaggtaagc cttcgcacat atcggtaaat agcttgcctg cttttattct ttctgtcatc
    24901 gacatgttca ttgcgatcat tccgccctga tatgttggtc ggataaggcg ctcgcgccgc
    24961 atccgacatt aattgcttaa gcgacttcat tcacctggcg acgcagcaag gaaagcgggc
    25021 cggggccgct aagcgtgaac acggaaatta aggtgaagcc cagcgccacc agacccagca
    25081 ccagataagc gccctggaaa ccgatgcttt catacatatt gcccgccagt acagacataa
    25141 aaatcatcgc cagttgctta aagaagcaga aacagaccag ataaatcgtc gctgaaaaac
    25201 gcacttcaaa ctggctggta atatatttaa agcagcccac cagcaggaac ggtacttcaa
    25261 acatatgcag cgttttcaga ataactactt ccagcgctga ggtggcgaac gatgagccaa
    25321 taatacgtac agacataata gtgccagcca gcagcagggc atttttccca ccgatgcgat
    25381 taatgatcag tggcgcaaag aacataatcg aggcattaag taattcgccc attgtcgtta
    25441 cgtagccaaa tacccgcgta ccctgttcaa cggtagcaaa aaacgaagta aagaaattag
    25501 caaactgttg gtcaaaaaca tcgtaggtgc aggaaacgcc aataacatac agtgacaaaa
    25561 accacagttt tggctgtctg aacagttcca gcgccagctt aaggctaaat gccgaatggt
    25621 tggcacctac cgcattggca accgtggctg aagagggcgc atccgttttg gcgaaaaaga
    25681 gtaaaacggc gaggatgagt gcacagccag aacccagcca gaaaacaaac tgattattga
    25741 tggtgaacat gatgccgaca atcgaggcac acagcgccca gccaacacag ccaaacatcc
    25801 gcgcgcgacc aaattcgaaa ttactgcgac ggctgacttt ctcgataaat gcctctactg
    25861 ctggcgcacc ggcgttaaaa caaaagccaa gataaatacc accaacaatc gatcctacta
    25921 aaatgttgta ttgtaacagt ggtccgaaga taaaaataaa gaacggcgca aacatcacta
    25981 acatgccggt aataatccac agcaggtatt tgcgcagccc gagtttgtca gaaagcagac
    26041 caaacagcgg ttggaataat agcgagaaca gagaaatagc ggcaaaaata atacccgtat
    26101 cacttttgct gatatggttg atgtcatgta gccaaatcgg gaaaaacggg aagtaggccc
    26161 ccatgataaa aaagtaaaag aaaaagaata aaccgaacat ccaaaagttt gtgtttttta
    26221 aatagtacat aatggatttc cttacgcgaa atacgggcag acatagcctg cccggttatt
    26281 attatttttg acaccagacc aactggtaat ggtagcgacc ggcgctcagc tggaattccg
    26341 ccgatactga cgggctccag gagtcgtcgc caccaatccc catatggaaa ccgtcgatat
    26401 tcagccatgt gccttcttcc gcgtgcagca gatggcgatg gctggtttcc atcagttgtt
    26461 gttggctgta gcggctgatg ttgaactgga agtcgccgcg ccactggtgt gggccataat
    26521 tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg gaagacgtac ggggtataca
    26581 tgtctgacaa tggcagatcc cagcggtcaa aacaggctgc agtaaggcgg tcgggatagt
    26641 tttcttgcgg ccccaggccg agccagttta cccgctctgc gacctgcgcc agctggcagg
    26701 tcaggccaat ccgcgccgga tgcggtgtat cgcttgccac cgcaacatcc acattgatga
    26761 ccatctcacc gtgcccatca atccggtagg ttttccggct gataaataag gttttcccct
    26821 gatgctgcca cgcgtgggcg gtcgtaatca gcaccacatc ggcaagtgta tctgccgtgc
    26881 actgcaacaa cgccgcttcg gactggtaat ggcccgccgc cttccagcgt tcgacccagg
    26941 cgttagggtc aatgcgggtc gcttcactta cgccaatgtc gttatccagc ggcgcacggg
    27001 tgaactgatc gcgcagcggg gtcagcagtt gtttttcatc gccaatccac atctgtgaaa
    27061 gaaagcctga ctggcggtta aattgccaac gcttattacc cagctcgatg caaaaatccg
    27121 ttccgctggt ggtcagttga gggatggcgt gggacgcgga ggggagtgtc acgctgaggt
    27181 tttccgccag acgccattgc tgccaggcgc tgatgtgtcc ggcttctgac catgcggtcg
    27241 cgtttggttg cactacgcgt accgttagcc agagttgtcc ggcgctctcc ggctgcggca
    27301 gttctggcag ttcaatcaac tgcttacctt gcgggccaac atccagaggc acttcaccgc
    27361 ttgccagcgg cttgccatcc agcgccacca tccagtgcag gaactcgtta tcgctatgac
    27421 ggaacaggta ttcgctggtc acttcgatgg ttcgcccgga taaacggaac tgaaaatact
    27481 gctgttggtg ttttgcttcc gtcagcgccg gatgcggcgt gcggtcggca aagaccagac
    27541 cgttcataca gaactggcga tcgttcggcg tatcgccaaa atcaccgccg taagccgacc
    27601 acgggttgcc gttttcatca tatttaatca gcgactgatc cacccagtcc cagacgaagc
    27661 cgccctgtaa acgggggtac tgacgaaacg cctgccagta tttagcgaag ccgccaagac
    27721 tgttacccat cgcgtgggca tattcgcaaa ggatcagcgg gcgcatttct ccaggcagcg
    27781 aaagccattt tttgatggac catttcggca ccgccgggaa gggctggtct tcatccacgc
    27841 gcgcgtacat cgggcaaata atatcggttg cggaggtgtc ggctccgccg ccttcatact
    27901 gtaccgggcg ggaaggatcg acagatttga tccagcgata gagtgcgtcg tgattagcgc
    27961 cgtggcctga ctcattcccc agcgaccaga tgatcacact cgggtgatta cgatcgcgct
    28021 gcaccatccg cgttacgcgt tcgctcatcg cgggtagcca gcgcggatca tcggtcagac
    28081 gattcattgg caccatgccg tgggtttcaa tattggcttc atccaccaca tacaggccgt
    28141 agcggtcgca cagcgtgtac cacagcggat ggttcggata atgcgaacag cgcacggcgt
    28201 taaagttgtt ctgcttcatc agcaggatat cctgcaccat cgtctgctca tccatgacct
    28261 gaccatgcag aggatgatgc tcgtgacggt taacgccgcg aatcagcaac ggcttgccgt
    28321 tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg
    28381 cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag agattcggga
    28441 tttcggcgct ccacagttcc ggattttcaa cgttcaggcg tagcgtgacg cgatcggcat
    28501 aaccgccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg
    28561 tttcaccctg ccacaaagaa accgtcaccc gcagttcatc gcgcagctcg ccgtacatct
    28621 gaacttctgc ctccagtact gcgcggctga aatcatcatt aaagcgagtg gtaacttgga
    28681 aatcgctgat ttgcgtggtc ggtttatgca gcaacgagac gtcacggaaa atgccgctca
    28741 tccgccacat atcctgatct tccagataac tgccgtcact ccagcgcagc accatcaccg
    28801 cgaggcggtt ttctccggcg tgtaaaaatg cgctcaggtc aaattcagac ggcaaacggc
    28861 tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc
    28921 catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaata ttaaatgtga
    28981 gcgagtaaca acctgtcgga ttctccgcgg gaacaaacgg cggattgacc gtaatgggat
    29041 aggtcacgtt ggtgtagata ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga
    29101 cgacagtatc ggcgtcagga agatcgcgct ccagccagct ttccggcacc gcttctggtg
    29161 ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    29221 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgcc gcaaggcgat
    29281 taagttgggt aacgccaggg ttttcccagt cacgacgttg taatacgacg gccagagaat
    29341 ccgtaatcat agtcatagct gtatcctgtg tgaaattgtt atccgctcac aatttcacac
    29401 aacatacgag ccggacacat agagtgtaaa gactggggtg cctaatgagt gagctgactc
    29461 acattaattg tgttgcgctc actgcccgct ttccagtcgg gaaacttgtc gtgccagctg
    29521 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg gaagggtggt
    29581 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
    29641 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
    29701 ggttaacggc gggatataac acgagctgtc ttcggtatcg tcgtatccca ctaccgagat
    29761 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
    29821 atcgttggca accagcatcg cagtgggaac gatgccctca tttagcattt gcatggtttg
    29881 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
    29941 gcgagtgaga tatttatgcc agcccgccag gcgcagacgt gccgagacag aacttaatgg
    30001 acctgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
    30061 cgtaccgtct tcatgggaga aaataatact gttgatggga gtctggtcag agacatcaag
    30121 aaataacgcc ggaacattag cgcaggctgc ttccacagca atggcatcct ggtcatccag
    30181 cggatagtta atgatcagcc aactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
    30241 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
    30301 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
    30361 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
    30421 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
    30481 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ccgcgacatc
    30541 gtatagcgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
    30601 tgccatacca cgaaaggttt tgcgccattc ggcggggctg acgccgaagt gagatttaaa
    30661 atgctgacgc caggtcacaa gtgagagaaa acccacgcca atacacctac ggcgtttaca
    30721 ggtatactcg ctaaaaatta ttcagcgggt ttggaaacaa agatggcaaa acttacctta
    30781 caagagcagt tgctcaaagc gggactagtc accagcaaaa aagcggcgaa ggtcgagaga
    30841 acggcgaaaa aatcgcgtgt tcaggcgcgt gaagctcggg cggcggtaga agaaaataaa
    30901 aaggcacagc ttgagcgtga taaacagctt agcgagcagc aaaaacaagc ggcgttggcg
    30961 aaagaatata aagcccaggt gaagcagctc attgaaatga accgaattac cattgccaat
    31021 ggcgatattg gttttaactt cactgacggc aatctgatta agaaaatctt tgtcgataag
    31081 ctcacgcaaa cgcagttgat taatggtcgt ctggcgattg cccgcttgtt ggtcgataac
    31141 aataacgaag gtgaatacgc cattattccc gccagcgttg ccgataaaat tgctcagcgt
    31201 gatgccagca gtattgtgtt gcacagcgcg ctcagcgccg aagagcagga tgaagacgat
    31261 ccgtatgccg acttcaaagt gcctgatgat ttgatgtggt aaacatagat aagccgtgca
    31321 gtgatgcctg atgcgacgct tacgcgtctt atcaggccta cggtttacgg gcgaagtgta
    31381 ggccggataa ggcgttcacg ccgcatccgg cagccgtgcg cagatgcctg atgcgacgct
    31441 tttcgcgtct tatcaggcct acggtttacg agcgaaatgt gggccggata aggcgtttac
    31501 gccgcatccg gcagtcgtgc actattatca acgcatattc agtttattgg cgtggtacgc
    31561 aatatgctcg ccaataaaac tggagacaaa ataatagctg tgatcataac cctcgtgata
    31621 acggattaac gtcttgatat tcatctcctg gcagatcttt tcaagatttg gagtccgcag
    31681 ctgttctgcg taaaaatcat cactcaatcc ctgatcaacc atgatttccg caacgcgttg
    31741 accttgtgaa ataagactca ccgggtcgta atccaaccag gcatctttat tttcaccaag
    31801 atatgcggca aaggcttgct gtccccacgg tacttgcgat ggggagacaa tgggcgaaaa
    31861 cgccgagacg ctggcatatt catctgggtt acgtaacgcc agcaccagcg cgcccagccc
    31921 gcccatagaa tgacctgaga tagactttct ggccgttgct ggaaaatgat gcatcactaa
    31981 attcggcagc tcgttgcgga tatagtcata cattttgtaa tgttcattcc acggcgcttg
    32041 cgtcgcgttc aggtaaaacc cggctccttg cccgagatcg taacggtcag catctgcgac
    32101 atgactgcct cgcggactgg tgtccggcgc aacaacaata atgttgtgct cagccgcgta
    32161 acgctgcatc cccgatttag taatgaaatt ctgctcgttg caggtcaggc ctgaaagcca
    32221 gtacaacacc ggcaattttt cattctcggc ttttggtggg agatagacgc cgacattcat
    32281 ttcacatttc agtgattggg aataatgccg atacacattt tgccagccgc caaagctggc
    32341 atgtttttca atgagttcca tcagtcatta tctcacggtt aatcccccgg acggctgcct
    32401 ggggagagta ttaagaaccc cgtctgaact ggacggggta aaccagcggg aaatcagtaa
    32461 cgaattacgg ttcgaatgga tttaccttca tgcatcaggt cgaaggcgtc attaatttca
    32521 tccaggctca tggtatgcgt gacaaacggt tccagatcga tatcaccttt catcgcatct
    32581 tcaaccatac ccggtaactg ggaacgacct ttcacgccgc caaacgcgga acctttccat
    32641 acgcgaccgg tgaccaactg gaatggacgg gtggagattt cctgaccgga gcctgctacc
    32701 ccgatgatca ccgactgacc ccagccgcgg tgcgcgcttt ccagcgctgc acgcatcacg
    32761 ttgacattac cgatgcattc aaaggtatgg tcgatacccc atttgttgat atccagcaga
    32821 acatctttaa ttggtttgtc gtagtcattc gggttaatgc agtcggtggc accgaagcga
    32881 cgcgccagtt cgaatttctt cgggttggta tcgatagcga taatccgtcc cgctttcgcc
    32941 tgacgcgcgc cctgaaccac tgccagacca atcgcgccaa gaccaaacac ggcaacagaa
    33001 tcacctggct ggactttagc tgtgttgtgt accgcgccaa taccggtggt cacgccacag
    33061 cccagcaggc agacgtgttc atggttcgct tctggattaa ttttggccag agacacttcc
    33121 gctaccacgg tgtattcact gaatgttgag catcccatgt agtgataaag cggctgcccg
    33181 ttgtaagaaa aacgggtggt gccgtctggc atcaagcctt tgccctgcgt ttcgcgaacc
    33241 gcgacacaca ggttagtttt gccagaacga cagaactcac actcgccgca ctctgcggtg
    33301 taaagcggga tcacatggtc gccaggtttg acgctggtta ccccttcacc gacttcaact
    33361 acaaccccgg ccccttcgtg accgagaacc accgggaaga caccttccgg gtcatcgccg
    33421 gagagggtaa atgcgtcggt atggcaaaca ccggtatggg tgactttaat cagcacttca
    33481 ccttttttcg gtggtgcaac gtcaatttca acgatttcca gcggtttacc gggagcaaat
    33541 gcaacggcag cacgtgattt catctctcgc tcttcctcaa tatggtaata gattcagcta
    33601 tttaagatag gcacgaacca gttcaatagt gtcgtcaacg gattggctga cttcgcggct
    33661 gtagcagtca tttcggtcaa acgtttcccg gatatggctt tcaagcactt ctgccatcag
    33721 cccgttagcc gcgccccgaa cggcagcgat ctgttggagt atggcacggc attcggcatc
    33781 accctccagc gaccgttcca gagcatcaat ctgcccccga atacgacgaa ctcgagtaag
    33841 gacctttttc ttctcttccg gagtactggg catttcgcac ctcatcatct gcacgcaata
    33901 tactataggg gagtatgcta tatatcaatg catacccccc tatagtatat aacactggag
    33961 aataaaattt atccggtgaa tgtagtcgga aaacaaagag gaaagggggt aggggctaat
    34021 cggcagggaa ggccgccccg gatagcgggc ggcagaagga atcagaattt ccaggtcaga
    34081 tgggctgcaa gttgcagacc gttataatca tcggttgggg tgtcgtacca cactttacct
    34141 gccgtcagcc cgagattaag ttgctcagtc agcggttgaa tacgttgtaa actgacgctc
    34201 ttcatgctgc cgtagcgtgt ttctggcgtc cagtcatagg tgtaagcgcc tgttccgcgg
    34261 cttagccata gttgcgtata accagtgccg cgcgggttat tcagacgcac ggaaatcata
    34321 ttgctgtaac taccacctgc atcgctggag tcataatggg tatagcggta gctggagatc
    34381 accgggccag tatagagtga tatgccgcct tgccaggcat cgacttcgac atcatcgtaa
    34441 tatttagtgt agcgataacc ggtcgtgaaa agcgtttttg gcaacagggc caggttaata
    34501 tcctgacgaa aatcctggcg ggcaaagacc ggcgtattat ccgcaaaggc aatacccgtt
    34561 cgggttgtca gccagttatt ccatttatac cagaccgcac cctgaccgcg agtggcattg
    34621 aaatgttcag tttcataatc acgacgcccc tgggaaagat taaaaagcag cgttgtgttt
    34681 tcaactttcg ccaccagttc agcatacgct aaattacggt tgccatgatc gccagaataa
    34741 tcggtaaaat cgtagcccgc ggtaatggaa gttaacccgg cataaacggg taatgcgcta
    34801 aaaattgccg ccgctaataa cgtccgtttt atcattttaa tctcctgtac ggataagttc
    34861 ttgtcggagt gaataagttc cggtaggtaa attacgttct acaatcacgc gcgggccaag
    34921 ttgcgtgttc ggggaaataa ttcgcccagg caaaataata acttgtacgc caaggcgtga
    34981 acgctgcccg atataacaac ctaatttatc gcatccggta gcgataattc cctctggagt
    35041 tcgaacagac acgggctgtt catccagacg atgattactg gttcgtactt gtgcgcccaa
    35101 atatgcctgg tttgcaacta ccgagtcggc aataaagcat tgcggaccaa t
//
LOCUS       DAZFRG010000019        34628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFRG010000019 DAZFRG010000000
VERSION     DAZFRG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.019"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHKUC_000727"
     CDS             complement(<1..186)
                     /locus_tag="ACHKUC_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6617027.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQK"
     gene            complement(183..566)
                     /locus_tag="ACHKUC_000728"
     CDS             complement(183..566)
                     /locus_tag="ACHKUC_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6617028.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEGVAE"
     gene            complement(806..1377)
                     /locus_tag="ACHKUC_000729"
                     /pseudo
     CDS             complement(806..1377)
                     /locus_tag="ACHKUC_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1449..1922
                     /locus_tag="ACHKUC_000730"
     CDS             1449..1922
                     /locus_tag="ACHKUC_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6617029.1"
                     /translation="MGEGSALPVGVPVPWPTATPPAGWLQCNGATFTKEQYPVLARVY
                     PTLRLPDLRGEFIRGWDDGRKIDEGRKLLSWQKGTLVGGHDDNDSSLDISYMSNGNNI
                     DYGGDKVFAGNYRSDYLWYAILGGTNSRAKAELNGAFFNITRPRNIAFNYIVRAA"
     gene            complement(1929..2543)
                     /locus_tag="ACHKUC_000731"
     CDS             complement(1929..2543)
                     /locus_tag="ACHKUC_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6617030.1"
                     /translation="MSNTAVLDENGIATVAGDITVYHYDEETREYTSSSVEYLALGVG
                     TPAHSCADAPPEAISGYVVCRTATLNGWEHVPDHRGETVYSTENGNPVLITQPGDYPA
                     DTTTKQPATPWDTWNGEAWVTDTERQRAAELEVARQQRQQRVKQAMASVDLINLKLRA
                     GRSLTPEETAKLNAVLDYIDELNALDISTAPEISWPEAPLALAS"
     gene            complement(2828..4174)
                     /locus_tag="ACHKUC_000732"
                     /pseudo
     CDS             complement(2828..4174)
                     /locus_tag="ACHKUC_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534505.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(4177..4755)
                     /locus_tag="ACHKUC_000733"
     CDS             complement(4177..4755)
                     /locus_tag="ACHKUC_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6617031.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
     gene            complement(4748..5851)
                     /locus_tag="ACHKUC_000734"
     CDS             complement(4748..5851)
                     /locus_tag="ACHKUC_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6617032.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFSREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     TIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTSTCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVDLLVSNLTASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMSGVSDD"
     gene            complement(5842..6189)
                     /locus_tag="ACHKUC_000735"
     CDS             complement(5842..6189)
                     /locus_tag="ACHKUC_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6617033.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            complement(6244..6840)
                     /locus_tag="ACHKUC_000736"
     CDS             complement(6244..6840)
                     /locus_tag="ACHKUC_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6617034.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLTIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGKGVTATIHGNIEIKGNAHATGSMLSDGENSSHHSH"
     gene            complement(6837..7991)
                     /locus_tag="ACHKUC_000737"
     CDS             complement(6837..7991)
                     /locus_tag="ACHKUC_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6617035.1"
                     /translation="MAEINSTAQVTSALTGVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVGFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            complement(7979..8191)
                     /locus_tag="ACHKUC_000738"
     CDS             complement(7979..8191)
                     /locus_tag="ACHKUC_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6617036.1"
                     /translation="MFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVLP
                     SGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            complement(8191..9075)
                     /locus_tag="ACHKUC_000739"
     CDS             complement(8191..9075)
                     /locus_tag="ACHKUC_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6617037.1"
                     /translation="MFAVLGDIEFELITYWDGFEATFGVDYAEHARIEGKPGLQFVGD
                     RLDEIQISLVLHQHYCVPDVELARVRTAMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRRQVPGAGAISGAVPSPSGVAQFVR
                     NGVNYAKQAQSVLQTTISTVRVAQKMKGNPVVALTRVPGLMSGLGNISGALGKSIPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAAGTAFN
                     RMSPGLSAMAARILTRSV"
     gene            complement(9075..11960)
                     /locus_tag="ACHKUC_000740"
     CDS             complement(9075..11960)
                     /locus_tag="ACHKUC_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6617038.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     LKYNQYQDELQEAVGSLISDNIDNVSDIGFLMPDIARAATATRTSAQDWAKVAAVWQN
                     SLKGAARDFGAVQNIMAYAGDQGAFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSIASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKTLMVSSRDLAITLGDQL
                     APSFISLTQELLPLIQGAKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVSVWKNAVLLRANWLRLSLALGQGGKLRWLVRGIMLAGRAVLWIGRALMMNPIGLV
                     ITAVAAAAYLIYRNWWAVSGWFKQRWADIQEAFNGGIVGIGKLLINWSPAGLLYKAFA
                     AALKYFGVALPAKFTDFGGHLIDGLINGIKNKWGSLKSSVTGMGDSISSWFKQCWADI
                     QEAFNGGIAGTGKLLINWSPAGLLYKAFAAALKYLGVDLPAKFTDFGGHLVDGLINGI
                     KNKWESLKSSVTGMGDSISGWFSEKLGIHSPSRVFMGFGDNIAQGAAIGLQRTTPLAA
                     LAGQRMASELLPKMPVSIQGPEIRDNTSGVRFSMPLPDINGFMSSAKNAIGAVINSLS
                     SMPAIPLSTRASILPGQRLANEMTPDVPRIPSPEIMAAGYSGRGAAATGGGTSGGIQV
                     SFNPQFFLNGKETAAPAGLAGALNMSLHELEKMLERLLAQKQRKEYS"
     gene            complement(12036..12194)
                     /locus_tag="ACHKUC_000741"
     CDS             complement(12036..12194)
                     /locus_tag="ACHKUC_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6617039.1"
                     /translation="MRATRALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            complement(12118..12453)
                     /locus_tag="ACHKUC_000742"
     CDS             complement(12118..12453)
                     /locus_tag="ACHKUC_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6617040.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESNQGADAGAGAAGE
                     MVPVSAGGD"
     gene            complement(12551..12832)
                     /locus_tag="ACHKUC_000743"
     CDS             complement(12551..12832)
                     /locus_tag="ACHKUC_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617041.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARKLADLIEAWEPPDHWFSGTGKSEGKTLLIAFLRNCKGFRTC"
     gene            complement(12835..13359)
                     /locus_tag="ACHKUC_000744"
     CDS             complement(12835..13359)
                     /locus_tag="ACHKUC_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6617042.1"
                     /translation="MMSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKAL
                     GMVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYKSLALQCRSSVQRYSSQGLIDE
                     IPLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRVG
                     GVDQLTDYRINIGG"
     gene            complement(13356..14783)
                     /locus_tag="ACHKUC_000745"
     CDS             complement(13356..14783)
                     /locus_tag="ACHKUC_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6617043.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIEDEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTSHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMAEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            complement(14773..15024)
                     /locus_tag="ACHKUC_000746"
     CDS             complement(14773..15024)
                     /locus_tag="ACHKUC_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617044.1"
                     /translation="MKEYRYSGPASGVTLSDGTEILLWPGKTVSLPEEHDYVKVLVAL
                     KHLTPVPEETKPAGTPAVQSPKRRSSSDSEVKTEDTDGS"
     gene            complement(15024..15488)
                     /locus_tag="ACHKUC_000747"
     CDS             complement(15024..15488)
                     /locus_tag="ACHKUC_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HFO6617045.1"
                     /translation="MNVLPVLDAVLARLREKLPQLQVEYFPEKPSEYRLNHSVGALLL
                     SYAGSRFDRPDDTGAVIQSQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGHTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            complement(15488..15934)
                     /locus_tag="ACHKUC_000748"
     CDS             complement(15488..15934)
                     /locus_tag="ACHKUC_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HFO6617046.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEEMDFDSPATVNTRVLDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            complement(15936..16274)
                     /locus_tag="ACHKUC_000749"
     CDS             complement(15936..16274)
                     /locus_tag="ACHKUC_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO6617047.1"
                     /translation="MGTTQQVILITTVTAGAALAQQRFVGADNTPCKAGAAALGVAEV
                     DAVTGDSTPVSVLGIIAVEAGAAVSRGVAVQSDAQARAVPQSGDGKSCGIALDEAGGE
                     GDVIRILRGV"
     gene            complement(16284..17237)
                     /locus_tag="ACHKUC_000750"
     CDS             complement(16284..17237)
                     /locus_tag="ACHKUC_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617048.1"
                     /translation="MSDYLKGKRVVDPVLTSIARGYKNAAFIGERIFPVVLTDKEGVR
                     VPTFGKTAFVEYDTERAVGADSNVLVREKTGTLDLVLGEHDLAAPVDYREQAESMFNE
                     ESKAIRRATNGVNLRRELIAARLAQDEKVYRTGHVKKLTASDRWAGGKGDPIGVIEAG
                     MEAVRTATGLRPNLMTMGAGVMALLKFHPAIQAAIGANERKRITTEILQDLFQIEEIV
                     IGAPVSLPSMKAAMDKNSVPADIWGDNLMLHYVGKPQPGADSADENEPSFGYTLRRKG
                     MPVADKYDGAGGKVKYCRYTDIYKVAVVGGDAGYLITGISK"
     gene            complement(17252..18367)
                     /locus_tag="ACHKUC_000751"
     CDS             complement(17252..18367)
                     /locus_tag="ACHKUC_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO6617049.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTLSQDAAGAQFSETGPGPSSASNEESSMT
                     KEEIEALQEENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VAEFAEADPERLALHQKAVALSKKEGISYEAAVARCL"
     gene            complement(18582..19040)
                     /locus_tag="ACHKUC_000752"
     CDS             complement(18582..19040)
                     /locus_tag="ACHKUC_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6617050.1"
                     /translation="MASDNLVSITINDKSLRRSLRALDLAATDLEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWMPSLAAEERDGQTLQDTGRLMGSVSTDYDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTGDGELQPEVVIPILDTIVRHLESAARR"
     gene            complement(19043..19864)
                     /locus_tag="ACHKUC_000753"
     CDS             complement(19043..19864)
                     /locus_tag="ACHKUC_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO6617051.1"
                     /translation="MSVTTAELAYCMTLPPKRAVSYLKSKGYQITWDWEEMWQEAHAR
                     AFTVAKVTRLDILEDIRGALQQAVDEGKTDRWFRQALEPVLKRKGWWGPRDTTDPVTG
                     EPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSVMGQELKLV
                     SEKTGEMRNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            complement(19845..21338)
                     /locus_tag="ACHKUC_000754"
     CDS             complement(19845..21338)
                     /locus_tag="ACHKUC_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO6617052.1"
                     /translation="MARGIWVSPDEFVAFSEPQKSLTAQIASRSRAIDFYGLGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAALKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRAWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVAAIPDDSSIELKAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQEAIDDTRATRDEKLTRAGLRLTPQYFMREYQLQEGDID
                     LSDAPVADGAVPAEFAEAISADHDAQQQLDDALDILMNGGVLNGTLEPVLAPLFRRVE
                     NGVNPSELLGELAELYPQMNTDDLQERLARILFVANIWGRLHERDNG"
     gene            complement(21338..22879)
                     /locus_tag="ACHKUC_000755"
     CDS             complement(21338..22879)
                     /locus_tag="ACHKUC_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617053.1"
                     /translation="MSPMDGRNNLAPVLPDTSALDAPPVLLPYQQRWVADTSPLKVIE
                     KSRRTGITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAA
                     SEIEEGFWEEDDDDKHIKTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQ
                     LDELLKAALAMLIWGGKVHVISTHNGDDNPFNTLIGDIRAGRQGGSIHRITFREAVSE
                     GLFRRVCLRTGKEWSEASEQAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESR
                     MSADTPVLRLTCKEGYELLSDEVRFRETQDWLDEYLKPLLEALPTDARSFLGRDFGRS
                     GDLSVDYPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNG
                     AYLAEYAMQRYGSSRIQQVMPTEGWYREHMPPVKAALEDGNLVDLPKDEDTLDDLRAV
                     QVVNGVPRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAAR
                     MLEGF"
     gene            complement(22930..23475)
                     /locus_tag="ACHKUC_000756"
     CDS             complement(22930..23475)
                     /locus_tag="ACHKUC_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HFO6617054.1"
                     /translation="MARRSTIEKLPEDVRRWLERALTESGFSGYNELESLLRERGYVI
                     SKSAIHRYGQKIERRYGAIRAATEAARMLTEGAADDQDARSEAVIALIQTELFESIVQ
                     LQEAEEGEVDPKERVALLSKVAKNVATLSRASVNLKKFQSEVRARAQQAASNAEKIAR
                     KGGLSSDAVQALRREILGIAT"
     gene            complement(23475..23786)
                     /locus_tag="ACHKUC_000757"
     CDS             complement(23475..23786)
                     /locus_tag="ACHKUC_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617055.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLDVSRAIYPDT
                     TPLELRRELDYLADRKMVELEKKPSGDWFADLTRLGVDLVEYTVECGPGIARPEKYWS
                     E"
     gene            complement(23786..24112)
                     /locus_tag="ACHKUC_000758"
     CDS             complement(23786..24112)
                     /locus_tag="ACHKUC_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617056.1"
                     /translation="MTLQVEFWTVVSFLLTFMGFVGGLAKWLFSKTEERQAARFASLE
                     QALQQSASNWGELEKEFMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            complement(24109..24759)
                     /locus_tag="ACHKUC_000759"
     CDS             complement(24109..24759)
                     /locus_tag="ACHKUC_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617057.1"
                     /translation="MWHLVKKLPWRGILLAILINAFLIGLYAMGYRSGHDSAKRDGNT
                     ALSQLQSAFDAYKTAQATLENAALRAWARRYQEQVAAGQRAEAGYLEQIAQLESRNKQ
                     LQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAATGT
                     RSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAWQCRRWRNQINALLDEREGLQK"
     gene            complement(24743..25471)
                     /locus_tag="ACHKUC_000760"
     CDS             complement(24743..25471)
                     /locus_tag="ACHKUC_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6617058.1"
                     /translation="MNWPQITLIILFAFGLGVTAIRHGEPRNDKYSFWWQLAGKLVIV
                     WLLWCGGFFSQARAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESGWRPD
                     AVSPAGAQGLAQFMPATADWISQLMPGLNSREPFNPAWAIRALVSYDRWLWQRVSAAN
                     DCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRENRHY
                     PQRILRELAPRYLTWGGSSCVASG"
     gene            complement(25474..25824)
                     /locus_tag="ACHKUC_000761"
     CDS             complement(25474..25824)
                     /locus_tag="ACHKUC_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO6617059.1"
                     /translation="MKSLKKFIPPVKKPRLSGWLLTSVLLLGTIALVSPQQLPVVIYK
                     LALITLAAVLGYWLDRSLFPKARPGQYLKHEDRLMAEGRFPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVATGL"
     gene            complement(26190..26765)
                     /locus_tag="ACHKUC_000762"
     CDS             complement(26190..26765)
                     /locus_tag="ACHKUC_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2441 domain-containing protein"
                     /protein_id="HFO6617060.1"
                     /translation="MGTYYTLDSASRLPSPCHVITGKYEPEMPELAAFLHQMYPNGLS
                     SHGYNYLYNPGPKMEDDSGISRSLLVGLVFELVRRSHFPDKPSRYQSLFACQHLSEVR
                     KFRELLADERGEDEIRTAPIYEVITNEQVHRGDMRLLNSDCPVLELYHRAWLYWSGEA
                     APVKTGEDEPFWELLIPLPVFVGRRITESQD"
     gene            complement(27175..27786)
                     /locus_tag="ACHKUC_000763"
     CDS             complement(27175..27786)
                     /locus_tag="ACHKUC_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617061.1"
                     /translation="MGLIKRYRTDYPNQVHQLSVAVSKHYYAGVDGLLKYQKKPFDIK
                     LSNASKTGKDHLLVYSLRDHYSGLFYIELAFLSTPQPITAFLGRAWRPKEDNVLQGLP
                     DMLMIPKTVEDEFPNVCNAVYNQGVDLLKVTSGFQSGGLIAIRAVEEDLKFYTNTPIA
                     RVREVAVKTCRYNANDIARTNKETKQEMWLRNVGQIDIPSPDF"
     gene            complement(27807..28793)
                     /locus_tag="ACHKUC_000764"
     CDS             complement(27807..28793)
                     /locus_tag="ACHKUC_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617062.1"
                     /translation="MSKEKQTVNGDVGNVVSGDVTIHNYSADILPSAQQPISLLQKRD
                     LHRLMDELVDLGESKRELWMTIHTKLNTKTVNEMTAADYHGAVEILQRYAQQIQNMKD
                     CNLLVSKIMVLTDPRYRLDRDRYCLKHFGTTHLKGLDKEQLQAVFGYFDDLLNIRDES
                     KTLSPPSDSGAKSVATVPTRSRKPHVVVLGGALVLVAIFFVGMIVFAGKWGNAKAVAS
                     TETSVFNIKTNDKIIEDSIPALRSMFPGLNKYSDDFHSVSSYKQKSGWHTLKFTVSTQ
                     AIIPESYGVKGKTCYININPDGRYARVLTAPCRALLLDQQNTPDSKYRYILK"
     gene            complement(28790..29251)
                     /locus_tag="ACHKUC_000765"
     CDS             complement(28790..29251)
                     /locus_tag="ACHKUC_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6617063.1"
                     /translation="MRTIVLIVNEVYVNFDSLCASRFKAERSRLSLKQAEVAALCGVS
                     REMWGKYERGVAVPGGELLAAFARIGANVQFILTGESSGITLSRDEMELLQHYRQAPL
                     QVKGSVLSALTTGSSRERAEQVIHGDVLGNVIKGNVTIGTGGIMNKNTKRK"
     gene            29302..29490
                     /locus_tag="ACHKUC_000766"
     CDS             29302..29490
                     /locus_tag="ACHKUC_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6617064.1"
                     /translation="MTSEQVKTLFRQRGITFTRWAEEHGYSRNEVYRVLNGQTKARYG
                     KAHEIAVKLGLKPTSNAA"
     gene            29543..29851
                     /locus_tag="ACHKUC_000767"
     CDS             29543..29851
                     /locus_tag="ACHKUC_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6617065.1"
                     /translation="MSKVNISSSGTRILRVLKALRGHSLNGVSNGELASALDESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            29862..30773
                     /locus_tag="ACHKUC_000768"
     CDS             29862..30773
                     /locus_tag="ACHKUC_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFO6617066.1"
                     /translation="MGRTKSPVSTELNVEVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERDRVVHEARFYMAQSAEAMLEAGKRLIILKENEPHGEFIKILESELGLAYRTSVR
                     MMQASTKYLSPTLKPNVPTLAHLGKAKLFELMTEDDEELAELADGGTVAGMTLDDVDR
                     MSVRELRQALREARETNAAQQRVLADKNEKIDSLSTRLEKKSRIQPPEPDEEVKKLRA
                     EVTALAVEAESAIAVRLSSAFETLCAYCTENMIDTPRDFMAGLVCQLESTARSLRSTF
                     DLPDEPTGDVAPSWLTEPTPQINGLEA"
     gene            30777..32546
                     /locus_tag="ACHKUC_000769"
     CDS             30777..32546
                     /locus_tag="ACHKUC_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFO6617067.1"
                     /translation="MNAALTERLVYVARAARDAGHGKRGAIYDAACAELGMSRATLLR
                     RLKEVSVTDKRKKRADAGRSALTRDEAALISATLREATRKNGKRLYSIADAVETLRAN
                     GFISAGRTDETTGEFFPLSEDAISRALRNYGLHPEQLDAPAPHTEVASLHPNHVWQID
                     ASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARIASDRVWSYEITDHASGWIYVEYV
                     TGAESGENLCSVLINAMQERGGADVLHGVPKILYLDPGSANTAGMTKNMCRSLGIDLI
                     AHKPHNARATGQVEKARDIIERKLEPGLKFRPVHSLEELNALAAKWRSHFNATAVHSR
                     HGKTRTDIWLKITAEQLKKAPSVEVCRELAVATPELRKVTPKLRVSFRGTEFDVSTVP
                     GVLVGEKLMITRNPWRSDVAQVVLTGEDGHETFFLVEEVRKNEFGFAEGAAVFGESYK
                     ALPETPAQTAAKETEALVTGTDNAADAAAARKAKALPFGGRLDPYKHIDDATLPACIP
                     KRGQVSDVRRPRTEQRPMTHVEAAKALRDKFSADGLTWTPEHYRQLTAQYPDGVPEAA
                     LDEVMATLTTPARSSVISIVNGN"
     gene            32557..33723
                     /locus_tag="ACHKUC_000770"
     CDS             32557..33723
                     /locus_tag="ACHKUC_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6617068.1"
                     /translation="MLVLKQQLKEARIPQAVVARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTAKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSDDVFTTPDIRYVREALYQTARHGGFMAVIGESGAGKSTL
                     RRDLTERINRENAPVIVIEPYIIAMEDNDVKGKTLKAAAIAEAIISTIAPLESIRRSQ
                     DARFRQLHRVLKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLSIVLI
                     GQPELATKLSERNMEVREVVQRCEVVELLPLDNNLEEFLTFKLQRAGKQLTDIMDASA
                     VDAIRARLSNPGSHRKNMVSLLYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
     gene            33726..33995
                     /locus_tag="ACHKUC_000771"
     CDS             33726..33995
                     /locus_tag="ACHKUC_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617069.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSTTIRGNKPVIRVSR
                     SAHCMRMLEQGKACYLYTGHDHRGYFRQGVFELHGCRVVWPESLW"
     gene            34023..34553
                     /locus_tag="ACHKUC_000772"
     CDS             34023..34553
                     /locus_tag="ACHKUC_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6617070.1"
                     /translation="MAKSTKGAKRIKSAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGE
                     VQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGITIR
                     SDIEDFSIIPFEQDVQ"
BASE COUNT         7796 a         9752 c         9101 g         7979 t
ORIGIN      
        1 cttctgttgc tcactcaggg catgtaaatc atgctcaaac cacgccccct gagcatctgt
       61 ctgcagaatc aaccggccat gacaggaaag gcagaaacaa tgccggatat ttctgctgag
      121 cgcagagtcg gcactgataa tgtgctcacc cggcgtgatc cccagccccg tttttatacc
      181 gttcattctg ctaccccctc ctcactgaaa cgcccggtct ggcagtgtgt gcagtactgc
      241 tccccataat aatcgtggtg acattgcctg cagtaccagc tggctttacg taccacgggt
      301 aaggcatccg ggacgaattg ctgcagacgt ttaatcaacc gtatttctct gcgttccggc
      361 ctgacataag gacattgctg cgcgtgctcc gtcagcccgt cgtcagtgtg ttcaaaccag
      421 ggccgttcgg tgtcgtattg cgggtggtat ctgagtgcac tgccgcaaag gtggcaggtg
      481 tagtgggcat aaggagcagt ttgtgcggta cgggcaccgg tcagacgtcc gttgccatca
      541 agtgcgagaa aagattttgc gtacatagta tatgttcctc tgcgccagac gacacacagg
      601 caacagccgt cccggtacgg gcagcgtggt caggtgtgaa tggcggtttt cagttcagtc
      661 tcagaaggga aaatcagtgt ggtcgctgtg aacacgtcat cacactaagg tcagataagt
      721 cgtctgttga tgcgccgcgc gataaatgca ctctatcttg tgcgataaat ggcatccacg
      781 cggcgtttca ttatttgaaa aagaattaca tttcaggttg cgttatgaca gtcccaacag
      841 ggtggtagcg gtagatcgtt gacagtccaa tatcatagat aattgccagc cgctttctgt
      901 catacccttt ctggagcaat cttgcgatct gctcatgctg ctccttcgtc agtttcgggc
      961 gacgtcctcc aactctccct tgtgctcgcg cagcagccag tcctgccagc gtgcgctcaa
     1021 ctatcagttc acgctccatt tctgccagtg cacccatcat atgaaaaaaa accttcccat
     1081 cggagtgctg gtatcgattg aatccgtcag actgcgaaaa ttgacacctt ttgcgcgtag
     1141 ctcttctgtg agagcaatga ggtgtttcat tcttctaccc aaccgatcca gcttccagac
     1201 caccaatgta tcccctcttt taaggcactt taaagcgcgt ttcaggcctg gtcgttccga
     1261 cttggttccg ctcattttgt cctcaaaaat ctgttcacat cctgcgcaca ttaatgcatt
     1321 gcgttgcaaa tctgtgtttt ggtcatttgt tgacacacgt atgtagccaa tcagcattaa
     1381 aaatactccc ttttgagtgg ttaaagtact gagataatgt ggggaggatt tgagagataa
     1441 acgttggttt gggggaaggc tctgcattgc ctgttggtgt accggttcca tggcccaccg
     1501 ccacaccgcc agcaggctgg ttacagtgca acggagctac gttcaccaaa gaacaatatc
     1561 ctgttctggc cagagtctac ccgaccctcc gtcttcccga tttgcgcggt gagtttatcc
     1621 gtggatggga cgacgggcgc aagattgacg aggggcgtaa gttgctttca tggcagaaag
     1681 gtacactggt cggcggtcat gacgataacg attcttcgct tgatatctcg tacatgagta
     1741 acggaaataa tattgactat ggcggcgata aggtgtttgc ggggaattac cgtagcgatt
     1801 atttgtggta cgcaattctg ggtggaacaa acagtcgcgc aaaagccgaa ttaaacggag
     1861 cattctttaa cataacccgc ccccgcaata tcgcgtttaa ctatatcgtg agggcggcgt
     1921 gataccgttc aactggcaag tgccagtggc gcttccggcc agctgatttc aggtgccgtg
     1981 ctgatatcca gtgcgttcag ctcgtcgata taatccagca cggcgttcag ttttgccgtt
     2041 tcttctggtg tcagactgcg accagcacgc agtttgaggt tgataagatc gactgacgcc
     2101 atcgcctgtt tcacccgttg ctggcgttgc tgtctggcaa cctccagttc tgcggctcgc
     2161 tgccgttcag tatcggttac ccacgcctca ccgttccagg tatcccatgg cgtggctggc
     2221 tgttttgtgg tggtgtccgc cgggtaatca cccggttggg taatcagaac agggttaccg
     2281 ttctccgtac tgtataccgt ctcgccgcgg tgatcaggca catgctccca cccgttcagc
     2341 gtggcagtcc ggcaaaccac gtaacccgaa attgcctccg gcggtgcatc ggcgcacgaa
     2401 tgtgccggag tacccacccc aagggcgaga tactccacag aggatgaggt gtattcccgg
     2461 gtttcctcgt catagtgata tacagtgata tcgcccgcta cagtggcgat accgttttca
     2521 tccagaactg cagtgttact cattaaactg ccctcacaat gtaattaagt gcaatgctac
     2581 ggggtctgac agtgataccc ttcgcatacc cgcttctgat agaggtcaga atagtagcct
     2641 gcataccatt gtctgacatt gccatgacag gatcgctgaa tgttgtcccg tccggcattt
     2701 taaagctgcc ctcacgaagg tctgtaatgg aatcttcatt gttgaatccc atgccgacta
     2761 tccccgaggt tcccgtagtg tcctggttat aataatccag cattgctgtt cttaacagcg
     2821 ttgccccttg tgcggacagc aacttacgtc ctgtatcaac tttgcgcccg tcgtcccatc
     2881 cacggataaa ctcaccgcgc aaatcgggaa gacggagggt cgggtagact ctggccagaa
     2941 caggatattg ttcttttgta aaggcgcgcc cgtcgcactt taaccagcct gctggcggtg
     3001 tggcggtggg ccacggaaca ggcacaccaa cgggtagcgc cgagccttct cctaaaccaa
     3061 ggtaattgag gacatctttt atactgccct tagcgataat agcgcgacca atagctgtaa
     3121 gggttgccag cgctgcgcgg tctgctccgg taaaataagg caaccgatct gctgcagtgg
     3181 caagtcctgc cagagcagtg agcgtggcat ctgccggttg ttttccgttt gccagatcgt
     3241 atgcagcctt gaccgctttc ggtgtggcgg ccagtatttc agacgtgctg ttgatggcac
     3301 tgctcaattg tacggtgcct tttgccgtca gcgaggcagc aggcactccc gttatctgac
     3361 tccacgggtg agtgtggctg acgggcgcct tgccggctgc aaggtcgtat gcagccttga
     3421 ccgccttcgg cgtggcagcc tcagtttctg attgactgtc cgtggcgctg ctcagttgta
     3481 cggtgccttt tgccgtcagc gaggcagcag gcactcccgt tatctgactc cacgggtgag
     3541 tgtggctgac gggggccttg ccggcagcca ggtcatatgc agctttgacg gcctttggtg
     3601 tggcggccag cgtttcagag gtgctgttgg tggcactgct cagttgtacg atccctttac
     3661 gggtcgttgt tgcatcctgg gctgtgtatt ttccccgggc cagatcatac gcgattttca
     3721 cagccttcgg cgtggcagcc tcagtttctg attgactgtc cgtggcactg ctcagttgta
     3781 cggtgccttt tgccgtcagc gaggcagcag gcactcccgt tatctgactc cacgggtgag
     3841 tgtggctgac gggcgccttg ccggctgcaa ggtcgtatgc agccttgacg gcttttggtg
     3901 tggcggccag cgtttcagag gtgctgttgg tggcactgct cagttgggtg aaccccttag
     3961 ctgtcagtgt cgcgtccggg tggttacggg aacgtacgtg ttcagccagt gactgtgcgg
     4021 cttcctccgt ttttttcttc agccatttcg tcctgtttgc cagttgccgg ggggctcgat
     4081 ttgatatccc gtcagcacca cccagaaccg gatctgatgt ctccagttga tagatatttt
     4141 cttcccactt ttctgtttca ttcaggtttc ccataatcaa ctgctcccgt ggttataact
     4201 gccatcatgg atggcggtat tgttatagcg aatggccaca gactgatact ccaggctggc
     4261 cagatggcag cgggccggtg caaatgcagc gagcgtctga cgcagcatcg cggcctgatc
     4321 gttagtaatg ggctgtttca gaataacgcg ataaaccgcc caggcggctg catctccatg
     4381 aacaaacagt ccgttatagc tgcgattgcc gtcgtaactg agacgacctg tgccttcaat
     4441 cagatccact tcaccaaagc caaaacggcg gataacttcc cggattgacc acggcgtccc
     4501 tttataccgg tgtagttcga tggctgcttt tataagcatg cggcgaacat cgtccgattc
     4561 cgccagctcc cagccatcgc cagacagtga gaactgctcg cccagccatg gcagcgcaga
     4621 actgtcgacg atatcgacga gaaagaccat cagtacgctc aggtcgatgt tatccagccg
     4681 ccctgccagt cttcccagcg tcctgagact gatatcaccc tcaagcggtg gcgggagttg
     4741 taatggctca gtcatcagac actccactca tgttaagtgt gatcgccgta cagtttgccc
     4801 attcgttttc tgccaccacc ctcagtgccg gtgtcaccag ttcaacctgg tacaccccgg
     4861 aaacggacaa tgcactgata atctggctgg ggacaatatc gcgtcccagc gtggcggcac
     4921 gtgatgccac ccagttctgt atggcgctgt tagcggcatc ttttacagac ctggcatcct
     4981 gatcacgata gatcgtaatc ctggcttcaa tggtgtaatc caccttcact ggtgttttag
     5041 cccgcactgt atcagtgagt ggcctgactt tctcatcaga gcagaaactc tctaccagcg
     5101 tgagaacacc gccgtccggc agaccggtac tgagcagcgg atacagatct acggtcccgg
     5161 gaaccgggga aagcacagca acatcgacaa tgttgggatg ggcctgcatg gcatgaaagc
     5221 ggtatgcgcc acggcttccg gcattggtga atgactccgg ggccagcctg atacgctccc
     5281 ggagcctgtc atcgtcttcc tgttctgaac cgccggaact ggccgtcaga ttgctcacca
     5341 gcaggtcgac gttatcaatc tcatcgagta actgactgac ctgcgcaggt tgccagccgt
     5401 taccagcggt accgggttcg gtacaggtgg acgtgacatt gaccagcagc aatccggcct
     5461 tcagtaccac atctgtatcg gtggcaaaaa taacgctgtc ggaagcgctg acgcgggtgc
     5521 ctgccgggat cagcacatca atggtcagtg cctcatccac agagaactga agtgtggtgg
     5581 tggcaggctg cgcggcaagg cggtatacac caaccagttc tccgaggtaa tcaatcatcg
     5641 gctctcgtga aaaggcgacc agattctgct ttgctgcctc ctgtaccgca accctgacca
     5701 gcatttcgcg ataggcccac agatcaatca gcagacgttc tgcctgtgcc gggtacagcg
     5761 ttttgccggt atccgcttca tacttcgcaa tcatttctgc tgtgattttg tcggcatcgc
     5821 gttcaataaa atcgggttct gtcagcgcca tagcagctcc tgagtccggg tctgtccgtc
     5881 tgagcctttc cagctcaccc ggagcgtaag atgttcgccg tcgacggcgg gtttaactga
     5941 cataagctgg cagcggggct cccagcgccg gatggcatcg acggattcgc gcaccacatg
     6001 cggaatggcc cggtctacag gccagtcgat ataaagatgc agattgctgc cgaactccgg
     6061 gcgatgcggg tcgctgccgc ggggagtccg caggataatt tgaatcgcct gccggatatc
     6121 atccagcccc cggacaattt cgccgggagc ctgcagggct ggttgccaga atactgaggt
     6181 cgttttcatg ggggcagtat tgcccccgtg cgggaacgct gatattaaag gcgtttaaga
     6241 agctcagtgg gagtggtggc tggaattttc gccatcagac agcatactgc ccgtggcatg
     6301 ggcattccct ttaatctcga tattgccatg gatggtcgcg gtaacacctt taccaccaga
     6361 acccgccatg cctccttcgt aaatcagctt acccctgaca cgcagatctc cggtaagttc
     6421 ggtttccggt gcgtcaatcg tggctttctg tgttttcaat accacatcag caccacactc
     6481 aatgacaata tgctcaatgc cgccccggat ggtcagagtg tgcgtcttcc ggttgtagct
     6541 gtactctgca ccatcagcaa accgggttcc ccggatattt ttgtcactga acggtggttt
     6601 atcgacgtct gagtacaccg cgcccagaat aacaccatcc tcgccgttgg catcgagcag
     6661 cacctcaacc tgctccccca cgtcagggag ccagtaatct ttgttatcct gggtattgcg
     6721 ctgcagcacg ttaagccagt ttgtgcgcag gttatcgcat tcaggcagac gaacgcgggc
     6781 ctgaaccctg tcggcatcaa cggcactgac cgtaccgacc tgacgagtga caccagtcat
     6841 tttttcttct ccttaatcac cgtcgatgta ctgccatccg ggtgataaac cgtgagtttc
     6901 tgagtttttt gttttttccc tcttgtgact ggcccccgtg ccacttccag ctctgtggtg
     6961 tagccgctgt tacggtcaaa cgcatggcgg gcagtggtta tgagccatgg cccggataac
     7021 tgcccaaaac ccaccagttc aattttgttg cctgctgtca gttgaggtgt tcccgtcagc
     7081 gtcagggagc cgttctgctg gtattcgtta tgtctggcca gtgctgaatc cgctttaatc
     7141 cgggcactgt ccgggtcgct gacgcggctg ttaactttaa gtgagtcagc gctggtaacc
     7201 ttaccacctt tgagcttttt gtcgctttca cgggtaccac catcagcttc gtagacgatc
     7261 agttttttac tgctgctttt ctggtgtttt acctttgcag atttatagac ccggttgatg
     7321 gtgtcacgca gggaaaagcg ggccacatcc tgcggtttta actgcctgac cggctcctga
     7381 ctgcgcagtg tggccagatg agaaaaaatc agctggtcac tgaccacttt cactgcataa
     7441 ccatactcgc tggccagccg gcgcagaaaa cccacgtcgg tttcagcata ctgggtcacc
     7501 cggtcgattc tgatggactc aatgctgccc accagtttca gtcggtgctt tctggcaatc
     7561 cgccccgcaa cagctgccag cgtggtgttc tcaaaaccac gactggtttt agtccgcagg
     7621 gcactgttaa ccgaggtggc caccccacgg atagaaacaa cggaagcggg cgaactcact
     7681 tcgatctcgt ctatagagaa cgtaccgcag gacagcagct tctcgccctg atagcccatt
     7741 ttcagcgtca gcgtgtcacc cttgcccgga taccatttat ccagccagcg gccatcggtg
     7801 tcgtccagct ccacctcaat ggtatcggac tcatttttga tgttatcgct ccaggtcaca
     7861 cgggtgacat aaggcgcgat atcagaggtg atgtttttct gcagatacca cagagtgaac
     7921 accggtgtca gcacatcgct gacgccggtt aacgctgatg tgacttgcgc agtgctgttt
     7981 atctcagcca tggggcaata tcctcttctg tacgggcttc ttcagcctca atcactggga
     8041 tcagtaacaa cagcccggag ggcagcaccg gcgtgatggc cacgtgcgga ttggcggcaa
     8101 taatccgggg atagcccagc gggtcaccgt agtactgcca tgccagcgaa tcccagcgct
     8161 ctccgtcacg ggtaacatgt tcaagaaaca tcacacactc ctcgtcagta ttctggcggc
     8221 cattgcactt aatcccggag acatgcggtt gaatgctgtg ccggcggcgt taagctgccc
     8281 ggaaacggta tccagagcac ctgcaatatt tcttttgtcc acaccactca gcgcagactg
     8341 tgcctgctgt acatacgtgg ctgcttcgct ggctgttctg gccagactga tggcatcggg
     8401 catggattca gagagtgcgt taaacgccgg aatacttttc cccagagcgc cggatatatt
     8461 acccagtccg ctcatcagtc ccggcacacg ggtcagcgcg acaacggggt tacccttcat
     8521 tttctgcgcc acccgaacgg tactgatggt ggtctggagt acagactgcg cctgtttcgc
     8581 atagttgacg ccgttacgga cgaactgcgc caccccggaa ggcgatggaa cagcaccaga
     8641 tatggccccg gcaccgggaa cctgcctgcg tattgccggc ggttgcagag gatttttcgg
     8701 gtcaccggtg tattcccgga gagacacggt ggcactgaca gccagcacgt taccggtgct
     8761 gtctgtctgc tcgctggttg cggtcacatc ggtaatcacg aaccagccgc gatagtcacc
     8821 gttgccgaag accagcgcca gtgcctgatg ggccttcatg gctgttcgca ctctcgccag
     8881 ctccacgtcg ggcacacaat aatgctgatg gaggaccagg cttatctgga tttcgtccag
     8941 cctgtcgccg acgaactgca ggccgggttt accctcgatg cgggcatgct ccgcataatc
     9001 gacgccaaac gtggcctcga agccgtccca gtaggtaatc agttcaaact caatatcacc
     9061 cagtacggca aacatcagct gtactcctta cgttgtttct gagccagcag acgctccagc
     9121 attttttcca gctcatgcag gctcatattc agggcaccag ccagcccggc aggcgctgcg
     9181 gtttccttac cattgaggaa aaactgagga ttaaagctga cctggatacc accagacgtt
     9241 ccaccgccgg ttgcagctgc accacggcct gaatatccgg cagccatgat ttcaggcgag
     9301 gggatacggg gaacatccgg tgtcatctca tttgccagac gttgccctgg taaaatcgat
     9361 gcccgggtgg agagcgggat agcgggcatg gaagacaaac tattgattac ggctccgatc
     9421 gcatttttag cagacgacat aaacccgttg atatcgggca acggcatact gaagcgaaca
     9481 cctgaagtgt tatcccgtat ttctggcccc tgaatgctca cgggcatttt ggggagcagt
     9541 tcactggcca ttcgctgccc ggccagagct gcaagcggag tggtccgctg caggccaatg
     9601 gcggccccct gcgcgatatt gtcaccaaag cccataaaca cgcggctcgg cgaatgaata
     9661 cccagctttt cactgaacca gccactgatg ctgtcaccca ttccggttac actggatttg
     9721 agcgactccc atttgttttt gataccgtta atcagaccgt cgacaagatg gccaccgaag
     9781 tcggtgaact ttgccggcag atcaacacca agatatttca gcgcagctgc aaaggcttta
     9841 tagagcagac ctgccggcga ccagttaatc agcagcttac cagttcccgc gataccgccg
     9901 ttaaaggctt cctgaatgtc agcccagcac tgtttaaacc agctactgat gctgtcaccc
     9961 attccggtta cactggattt gagcgacccc catttgtttt tgataccgtt aatcagaccg
    10021 tcgataagat ggccaccgaa gtcggtgaac tttgccggca gagcaacgcc gaaatatttc
    10081 agcgcagcag caaaggcttt atagagcagg cctgccggcg accagttaat cagcaactta
    10141 ccaattccca caatgccgcc gttaaaggct tcctgaatgt ctgcccagcg ctgtttaaac
    10201 cagccactga ctgcccacca gttgcggtag ataaggtaag ctgctgccgc gacagcggtg
    10261 ataacgagac cgatgggatt catcatcagc gcacgtccaa tccagagaac agcacgtccg
    10321 gcgagcataa ttccgcgaac cagccagcgg agcttaccgc cttgccccag tgcgagcgac
    10381 agacgaagcc agttggcccg aagtaaaaca gcatttttcc agacacttac aaagggggaa
    10441 ataaggagat tcagccccag tttgagacca acagtcgcta tcttaatcgc aaggagcgca
    10501 ctgatgagct taaaagcccc actgacaaat tgcgggtgag tcgctaccca gtgttttgcc
    10561 ccctgaatga gtggaagcag ttcctgagtc agagaaataa aagatggagc taattgatcg
    10621 cccagcgtaa ttgccagatc gcgactgctg accataagtg ttttagtggc ttcaagggga
    10681 gatttcagcc gctgatcata agcactggca agcaaatcgt tatccgcagc cctgagagca
    10741 ccggcacgga tctctcgata tctgtccatg ttggccagca tcgggcggat aaatgccatg
    10801 acctgcatat ccgcgaacat atcgcccaga ccaaagtttt tcgccagagc ctgaagtgcc
    10861 tcatctcttg ccgtatcatt ctttattttc atggcggatt tgaagccggc cagcgcttcg
    10921 gggcttttgg cattgaggta acgttctata acactcaaca tcccttcaat cggagagatc
    10981 cccgcagctt tataactcgc aatagatccc tgcaaatcaa tacccagatc agcaaactgt
    11041 ttctgagtat cgcgggcaaa aattttggta agaaagtttt taaaattatt ggctgcttcg
    11101 tcggtggaac ctgcaccgat ttttgctatc tggagactgg ccccgatttc agcaacagcc
    11161 tcttttccac tggcaatacc cgccatcatt ggggccaggg actgcatcca cttgacctga
    11221 tccgggattt caaatgcccc ctggtcaccg gcataagcca taatattctg aacggcacca
    11281 aaatctctgg ccgcaccttt cagggagttt tgccagactg cggccacttt tgcccagtcc
    11341 tgagcagacg tgcgtgttgc cgttgccgcg cgggcaatat ccggcatcag aaaaccgata
    11401 tctgacacat tatcaatatt gtcgctgatg agtgaaccca ccgcctcctg cagctcatcc
    11461 tgatactgat tgtatttaag cgcccagcct tttatctgtc cggcaagtgc atcccgcgtt
    11521 ttattgtcat attttgcggt gatcgacata tcaatcattt tgtcctgaaa agacatggac
    11581 tgctgaacgg caggggaaac cgtatgataa acagtctgtg ccatgccata cgcttcaatc
    11641 ccctgaccat acagcgccat gcggttagct ttcagcgcat cactggtagc ggatgccgct
    11701 gacagacggc gctgctggcg ctcaatttgc tccatggtgc ggcttacccg cagcagctcg
    11761 ctgttgagat gctgcatccg ggaagaaccc agttgaccat aacgttctgt tgcacgggtt
    11821 aaagcgttct gacgttcctg caggcggcgt gaggtatcgc tcagggaatc aagcgcacgg
    11881 cgggtaccgc tcattgcaga acggaatgtg ctgccaatca tcccgccgat aacaacgccg
    11941 actgaaaact gtcccgacat agtggttaac ctccggggaa ggtgaaaaga cgtgagggga
    12001 taacgcagaa cagccgctac tggcggctgt ctgtactatg atttgtcgcc gtactcgctt
    12061 tttatttgct cttcagcctg ctccagccac atctccagat cgtcagtatc gagggcatca
    12121 atctcccccg gctgaaaccg gaaccatctc gccagcagcc cctgcgcctg cgtcagcgcc
    12181 ctggttgctc tcacccatcc ccgtgatgag ctgaaatcgt ttctgtaact gcaggtaatc
    12241 agccagatcc atattgtcga gatcttccgg gagaagacca gtactgcggg caatcagcgg
    12301 ttcgtcccag tctgccgggt ttttgctgat tttgcgcacc tgcttcaggt cttttaccgt
    12361 caggcgtttc agttcaacca gctcaattct ggtacctgca gcagtggtga agggataaga
    12421 caatttaaaa gtatcggatg gggtctgtga catgattgtg ctcctgtgta agttcagggc
    12481 agtatgtcgg gagaagcgcg tgacggatat taaaggagat taagaagaag gggccggagc
    12541 ccctgtgatg tcagcaagtg cgaaacccct tgcagttacg caggaaagcg atgagaagcg
    12601 tctttccctc agatttgccg gtgccggaga accagtggtc gggaggctcc catgcttcaa
    12661 tcaaatccgc cagtttgcgg gcctttgaac gtgtgcagtc aatcgggtca ttggttttac
    12721 gggtattaaa aagggtttcc acccccggaa tatcaaggag ggtaaaccac gtaccatttg
    12781 acatgcccag tgccgcacat cgtcctcctt tatccgtcag ttcaacactc accgtcagcc
    12841 cccgatattg atccgatagt cagtcagttg atcaacgcca ccgacgcgga agatgttggc
    12901 cagatagtcc agttgcagca actcttcacc atccagtacc tgtctgatat acgtgcaggt
    12961 gaagctactg gagaactcgg cgttctcgtg ctgtttaaac gtccccagcg ggttcttctt
    13021 gaacataatt gtcaggaagg tgaccagcgg gatttcgtcg atcagcccct gcgagctgta
    13081 gcgctggacg ctggagcgac actgcagcgc cagcgacttg tacgggttcg cggcagacag
    13141 catcgcatcg cggtaaaagc tgttccattt gatttcgcct tccagtttgt caaaaccagc
    13201 cgggagttcc accttaccca ccatccccag tgccttgtgt tcctgcataa tcatggagac
    13261 atcggggagt ttaacttcct cagcccgtcc cagcaggtta gtaccatcca gatagatgtt
    13321 ggcattcgtg atgcggttta tctcaatctt tgacatcagt tgcccccttt cagggttaac
    13381 aggtattccg aggtgatctc agtctcaaac gtcagtcgct ccagcggcgg tggtggcgta
    13441 tatttgtagc tcagcaacag gtgcccggcg gccagctccg tctccggatt gcgggcagga
    13501 tcaaaccagc aacggaagcc cagtaccgca ccatcaccag tcattttgcg accgtaggcg
    13561 ttgaccgact ccgtcagtgc atcaatcagc gcctgagtaa tcggcatgtc gatgtactgc
    13621 tggctgaaat aacgaatgga ctcgttgatc acatcaccgg tgcggcgaac gttctcaaag
    13681 ttacgcatat gggtgaccgt tggccatgct gccgtccggt taccccacag acgaaggccg
    13741 ctgccgtaac tgctgaatac cgtggtgatc ccctgttcgt taagcaggtt cacctcactc
    13801 tgcgggtcat caatcatcgc ggacagctgg cgctccacgc cggtgatccc cagaatctcc
    13861 tgattggagg atgaccacca gtagcccttg tccagatcga ctctggcacg cagacctgct
    13921 gcacgctggc tgagcggctc cagacgctct gtgtttgtca ccgggtcata taccttcaca
    13981 tgcggatagc acagacggac gcggtcggag ctggtattga agttgatggt gccttccggg
    14041 ccacgacctg ccagagcctg tgcaaaggtg gtaccgacag gcgcatcaat gtaggttacc
    14101 gcgcccagct tctctgccat ggcgataagc tcaactgcga cactcttctg ggtgcagaac
    14161 accggcgcaa tcagaatttt ggcgaaatag ccgtacaggt tgaagctgtc gttaagcagc
    14221 ttcatgccgg ttcggtttcc ggcgctgttc accccgccga tgatgtccgc cgcagtcacc
    14281 ttcgacgggt ccgcgtactc atagctcacc ttcacgctgc caccggcctc aatagccttg
    14341 cccaggttcg tgagcacacc cgcctgcgca tcaacacggt agtccgtgtt cgccgtgtgg
    14401 gaggtgctgc cttcactgtt tttcaccacc acattagcga caaccggatg cgccagtctg
    14461 gcctgccctg tcgatttgtc aaaggtaacc acctcatcct cgatcgccgt tttatgtttc
    14521 gccgggtcga ggacgttaat gaccagaacg gtacctgcac catggtcata aatcgcatcc
    14581 agcgcctgcg gaatggtaaa gccggtgaac tggctgccaa atgccgctgc gtctttctca
    14641 gacaggcact gtaccagcgt attgacatcc cccatcgggg cggtaccaat caggccaata
    14701 acggcagatt tcaccgtttt aaccgggcgg gcaccgtttt ccacctcaat ggtttcggga
    14761 ccatgcagat agttagctgc catcggtgtc ctccgttttc acttcgctgt cactgctgct
    14821 tctgcgcttt ggtgactgca cagccggtgt gccggcgggt ttagtctctt caggtaccgg
    14881 cgtcagatgt ttcagcgcca ccagtacctt cacgtagtca tgctcctccg gcagggaaac
    14941 cgtcttcccc ggccagagca ggatttcggt tccgtccgac agcgtgacgc cgctggccgg
    15001 gccggaatag cggtattctt tcatcactcg ctttcctcat agttcacttc ggttaacagc
    15061 gggccggacg gtaaatcgct gtcttcgata aagacgcttt cagtcgcgaa gtcgagggcg
    15121 tattgccaca gccccctgac ttcaccgata aacacctcgc gggtcagcca gatacggcgg
    15181 cggcagccgg gcggggtgtg gccaccgaga atgcggcgga cagcatccag gacattaatc
    15241 gccccttttt taccgttgag ctggcggaag accaccgtga cgcagagctg gatagtctga
    15301 gactggatca ccgcaccggt atcatccggc ctgtcaaagc gcgatcctgc atagctcagc
    15361 agcaacgccc cgacagaatg attgaggcgg tattcggacg gcttttccgg gaagtactca
    15421 acctgcagct gaggcagctt ctcgcgcaac cgggccagca ccgcatcaag gacgggcaga
    15481 acattcatca gtatttctcc agtaagccgt cacgaccgcc gaaagtggcc gggcgactgc
    15541 gtacacgaaa ctcgccaggc tcaggcacat ctttctgagt ggacggcagc cccagcgtga
    15601 gcctgttatc acgtaactcc ctgagttgcc gcagcgcttc tttgtggtca tccttcaccg
    15661 tatccgggag gtcaccttcc gggcggcggg cgtagagccg gtaacggacc agcgtgatgg
    15721 caatgtcccg cagaacagtc ggtatctctg ccagtggcag gatatagcgt ccgcgcagat
    15781 gggcatcaat cagctcgtcg gcatagcgga tacagctgtc caacacacgg gtattcactg
    15841 ttgcaggcga gtcgaagtcc atctcttcac tggtgagctc gataagcgtc cgctccggca
    15901 cctgcgcaag caaatcctcc agggtgcagt acatgtcaca ccccgcgcag gatacgaatg
    15961 acgtcgcctt cacccccggc ttcatcaagt gcaataccac aggatttacc gtcgccggac
    16021 tgcggcacgg ctctggcctg agcatctgac tgaacagcca caccacggct gacagcggcc
    16081 ccggcctcga cagcaataat gcccagaacg ctcaccggcg tgctgtcgcc ggtaacagca
    16141 tccacttcgg caaccccgag cgctgcggca ccggctttac agggggtatt atctgccccg
    16201 acaaaacgct gctgtgccag tgctgcccct gccgttacgg ttgtgatcag aatgacctgc
    16261 tgagtggttc ccataacgcc tccttattta ctgataccgg taatgagata cccggcatcg
    16321 ccaccaacca cggcgacttt gtagatatcg gtataacggc agtacttcac cttgccaccg
    16381 gctccgtcgt atttgtcggc aacaggcatc cccttacgac gcagggtgta gccgaaggac
    16441 ggctcgttct cgtccgcgct gtccgccccc ggctgcggtt tgccgacata gtgcagcatc
    16501 agattgtctc cccagatatc cgccggcacg ctgttcttat ccattgccgc tttcatggac
    16561 ggcagggaga ctggggcacc gatgacgatc tcttcgatct gaaagaggtc ctgcaggatt
    16621 tctgtggtga tgcgcttgcg ttcgttggct ccgatggcgg cctgaatcgc cgggtggaac
    16681 ttcagcagcg ccatcacgcc ggcccccatg gtcatcaggt taggacgcag ccccgtggcc
    16741 gtacggaccg cttccatacc ggcttcaatc accccgatgg ggtccccctt accaccggcc
    16801 cagcgatcac tggctgtcag ttttttgacg tgcccggtac ggtagacctt ttcatcctga
    16861 gccagacggg cagcgataag ttcacggcgc aggttcacgc cattcgtggc gcgacggatg
    16921 gccttgctct cttcgttaaa catggactcc gcctgctcgc gatagtccac cggcgcagcc
    16981 agatcgtgtt cacccagcac caggtccagc gtgcctgttt tttcacggac cagaacattg
    17041 ctgtccgccc cgacggcacg ctcggtgtca tattccacaa aggcggtttt cccgaaggtc
    17101 ggtacacgca cgccttcctt gtccgtcagc acgacgggga aaatacgttc gccgatgaat
    17161 gcggcatttt tatagccacg ggcgatactg gtcagtaccg gatcaacgac acgtttacct
    17221 tttaagtaat cagacatgct ctctccttaa attacaggca gcgtgcgaca gcagcctcat
    17281 agctgatgcc ttcttttttg gacagggcca ctgctttctg atgcagggcc agacgctcag
    17341 gatcggcttc cgcaaactct gccacatcca ccttcaccgt gtcgccgaca cgctcttttg
    17401 tggcctgttc ggcgaaattc atcaccggct ccccgtcgga gagcaatgaa cgaaaggcgg
    17461 tggccagcgg tgtgcggctt tccccctcag caaactccac cggcttgtcg ccaccggcga
    17521 cggcatccag cagggcaacc accacggagg aggcgcgggg agccagacgg ccctctgcga
    17581 ccagtttttc tgcaaaggcc acattgtcct tatgcagttg ctcctgtctg acctgtgcat
    17641 cacgcgcatc gcgatcagca gcctgctgct tcaggcggcg gttctcctcc tgaagggctt
    17701 caatctcttc ttttgtcatc gatgattcct cgttacttgc ggaagacggc cccgggcctg
    17761 tctcactgaa ttgtgcacct gctgcatcct gcgacagtgt gtcgcggtac gcctcttcgc
    17821 gcagactgtt gagctgccat tccggcagga ctttttctgc ctcgtccaga ctgaagcggg
    17881 cgatcagaaa atcgcgcagc tttccccaca gggaggcatt cgtgatggcc tgccagtcgg
    17941 cgaactccac cacaccttct tcctgctcac tgaaggacac ctgtttcagc cccttgacgg
    18001 aaggtggctg tgccccgaga aagcccacat ggcgaaggta gagcacgccg ggcttcggat
    18061 tggacggtga atccgggagg tagaaagagg cggacacttt tttgaatcgt ccgtcggtga
    18121 ccatctcagc aaactgcggg tccagctggg caggctctgc catcagatcg acgccgctga
    18181 gcgacagggc tttcacccag ccccacgccg ggtcttccgt tctgggatga ccaatcacga
    18241 gtggtgcttc atggacggac gggtcatagg ctttcacgca ggcggcaaga tcgtctggcg
    18301 tgaacggcag ttttttgccg tgcatatcgg tatgagtacc ggctttaaaa atgtgaatgg
    18361 ctgacatttt gctgtcccgc gttatgttgt cggagacagt ttgtgagaaa tgcaggcccg
    18421 gcgattttaa tctgctttag aaaacatcag gggagaagga cagggaaagc aatacggtga
    18481 accggaggcg gttataaaac agaggctgta aagcctttat aaaggtaata cagcccctca
    18541 ttcgctggca atgataaatc actcgcctga agagagaaaa ctcagcgacg ggccgctgat
    18601 tcaagatggc ggacaatggt atcgaggatg gggattacca cttcaggctg tagttctcca
    18661 tcccccgtca ccggcaggaa cgggcgggcc ggaagttcaa cagactcatt acgccccgtt
    18721 ttacccccga actggtgaat ggcaccgtaa acaacgttgg tccccacaac agcctgccgg
    18781 tcgtcatagt cggttgatac tgaccccatc agacgcccgg tatcctgcag tgtctgcccg
    18841 tcacgttctt ccgctgccag cgagggcatc caccccggac gcccctcatc aagaaagtta
    18901 aactgtgttt ccgccagcag ggttccggcg attttgcgca tcgcgggctc caggtctgtg
    18961 gcagcaagat ccagcgcacg gaggctccgg cgcagggatt tatcgttaat ggtgatactg
    19021 accaggttat cggaagccat tgttatcctc tcagttcctg ttgtgccagt ggctgaagcg
    19081 taccctgata gcgggccagg tcgggacggt atgctgcccc cggtgcataa gaccagccga
    19141 cgtcggtggt caccttcgtg gtgccggtat taaaggtggc cacgttccgc atttcgccgg
    19201 ttttctctga gaccagtttc agttcctggc ccatgacaga gccggagctg ataaccttca
    19261 ggccacgggc acgtacatcc gccgcactca gggcaatcac actacagcgg cagcgccagc
    19321 cgttcggcgg gtaaaatgcc tgccagaacg ggtcatccca gcgcagcacc agaccatgca
    19381 gcgccagatg gctcctgcgg gtatggctgt cgttgatgcc ggtatacatc cagtacggcc
    19441 tgtcgtcgac gttttccatc tgctccgccc aacgaccggc gctgtagagt acggacatat
    19501 tggtgcgaaa gatggtatcg agccgccacg gactgccctg ctgaatggtg accggctcac
    19561 ccgttaccgg gtcagtcgtg tcacgtggtc cccaccatcc cttacgcttc agcaccggct
    19621 ccagcgcctg ccggaaccag cgatcggttt ttccttcatc gacagcctgc tgcagtgccc
    19681 cgcgaatatc ttccaggata tccaggcggg tcactttagc gacggtaaag gcgcgggcat
    19741 gggcttcctg ccacatctcc tcccagtccc aggtaatctg ataccctttg gacttcaggt
    19801 agctgactgc ccgcttcggg ggaagcgtca tgcagtacgc cagttcagcc gttgtcacgc
    19861 tcatgcagac gcccccagat atttgccaca aagagaatgc gggccagccg ctcctgcaga
    19921 tcgtccgtgt tcatctgagg gtagagctcc gccagttcgc ccagcagctc agacgggtta
    19981 accccgtttt cgacccgcct gaacagaggt gccaggacgg gttccagcgt gccatttaac
    20041 acacctccgt tcatcagaat gtccagcgcg tcgtcaagct gctgttgtgc atcatgatcg
    20101 gcgcttatcg cctcggcaaa ctccgcaggc actgccccgt ccgcaaccgg tgcatcagag
    20161 aggtcaatat ccccctcctg cagctggtac tcccgcatga agtactgcgg ggtcaggcgt
    20221 agtcccgcgc gggtgagttt ttcgtcgcgg gtggcacggg tgtcatcaat ggcctcctgt
    20281 tcccacatgg cccagaccgg acacggcaca tcgccgaagt tcagggtgac caccgttctg
    20341 atgacctgat tcactgccgc ctgaatgatg tccgcatccg catcgcggat atcagccgtc
    20401 acctccagcc cggcctgtgc agaggccttg ttactgttcg cttcagtggt ctgattctga
    20461 ccgagtaatg caatggagat ctcactgcgt gacagcgtga tcagctcgcg gaatacctcg
    20521 ctgctgtccg ccttgccatc cgcggctttg agttcaatgc tgctgtcatc ggggatggcg
    20581 gccacagcgt cctccaccat ctgctccatg gagtccagca gtttttcaat ctctgcatca
    20641 tttgcacccc tcgggtgctt accgatcacc cacggggagc caaacttttc ggcaaagcgg
    20701 agccagaatt tcatcccgcc tttcttgaag gcgaccggcc agaagcacat ggacaggtcc
    20761 gggaaaccgt aaggattgtc atacgaggca tcctgtgccg gcaccacgaa ttttgacggt
    20821 gacagcagct caccctccac acccgcatca cgcgcccgga agcgcaggca gttgtccgta
    20881 tcaaactgaa accactcagg cggtttgccg acaatatccg tcaccgccca cgccctgacc
    20941 gaacggcccc acatgatttc acagggctga tacccgtaga gcacggcatc gctcatctca
    21001 ccgatgatgc gggacagatc cagatcgtcg agcatgtcgc ggatgaaacg gaagacccgg
    21061 gcagaagcgt gaccgcgctc cagtccacgc tccagcgatt tgagcgctgc tttacgtctg
    21121 cggatacagc ctccgaccag cgggtcggtg cgcagttcgc ggtagatacg gatatcccgt
    21181 ccctgagcct tgagaatggg atcaggattg ggcagataca tgcccagtcc gtaaaagtcg
    21241 atcgcgcggc tgcgggaggc aatctgcgcg gtcagtgatt tctgaggctc agaaaaagca
    21301 acaaattcat cgggtgaaac ccagataccc ctcgccatca gaatccctcc agcatacggg
    21361 ccgcctgacg acgacggcgt gagcttgcct tcaccggccc tttgttaatt tcacggctgg
    21421 cgaaatacgc cagcgccagt gcgatggctg aatccccgtg gcgtttacca ccgtcagcct
    21481 ttgcttttga gcgttgctcc ggtacgcggg ggacaccgtt caccacctga acggcccgca
    21541 ggtcatccag tgtgtcttca tcctttggta agtccaccag gttaccgtct tccagtgcag
    21601 ctttgaccgg aggcatatgc tccctgtacc agccttcggt tggcatcacc tgctgaatcc
    21661 ggctggagcc gtagcgctgc atggcgtatt cagccagata ggcaccatta ccacgggcat
    21721 caaacgctgc acccagcaga ccgggcaggc catccatcag ataccaggtg atttgctcct
    21781 gctgtctgaa cggcacgtta cgcagctcca gtacgaatgg cacgcgtcgt accaggttct
    21841 tctcctgcag caggggatag tccaccgaca aatcaccgct acggccaaag tcgcgcccca
    21901 ggaaagagcg ggcatcagtg gggagtgcct ccagcaatgg tttcagatac tcatcaagcc
    21961 agtcctgcgt ctcgcggaag cgaacctcat cagacagcag ttcataacct tccttgcagg
    22021 tcagacgcaa taccggcgta tcagcggaca tgcgggactc tatcagggca cgggacagcc
    22081 aggcaccgcc accgttggcc ggaatacagt caagctcttc ggatgcgccg gcaccgtaga
    22141 atttgtacac cgatgccatc caggcctgct cggaggcctc cgaccattcc ttcccggtgc
    22201 gcagacagac gcgccggaac agcccctcag atacggcttc ccggaaagtg atgcgatgta
    22261 tgctgcctcc ctgacgtccg gcacggatat ccccgataag cgtattgaac ggattgtcgt
    22321 caccgttatg ggtggagata acgtgtacct tcccccccca gataagcatc gccagcgccg
    22381 ctttcagcag ttcgtccagt tgctcatgga acgccgcttc gtcgataaca ataataccct
    22441 gacggccacg caggttagac gggcggctgg agagcgcaac aatacgaaag ccggagtcag
    22501 gaaatttgat ggtgtaagtc ttgatgtgtt tgtcgtcgtc gtcctcttcc cagaatcctt
    22561 cttcaatttc actggccgca tagttgaatg cccgtgccca catcgcacac gcctgaatgt
    22621 attcgacggt catgtcctgg ttataagcga tgtaatacac attcatcccg cctgctggcg
    22681 cagaagaggc ggcggtcagt acgttatcgg atgcctcagc ccatgtaata ccggtacgac
    22741 ggctcttttc tatcacctta agcggagagg tgtctgccac ccagcgctgc tggtaaggca
    22801 acagaacggg aggggcatcc agcgccgagg tatcaggcaa aacgggagca aggttattcc
    22861 tcccgtccat gggactcatc ccttcggggc cgccgccagc ggcgtttaaa atcactccgg
    22921 atgattttgt catgtggcaa tccccagaat ctcgcgacga agcgcctgta ctgcgtcgct
    22981 tgacagtccc cccttacggg caattttctc ggcgttgctg gctgcctgct gcgctctggc
    23041 ccgtacttca gactggaact ttttgaggtt gacggacgcg cgggacagcg tagccacatt
    23101 cttcgccacc ttcgacagca gggccacgcg ttctttagga tcgacttcgc cttcttccgc
    23161 ctcctgcaac tggacaatac tctcgaacag ctcggtctga ataagggcta tcacagcctc
    23221 cgaacgcgca tcctgatcgt ctgctgcgcc ttcggtcagc atgcgggccg cttctgtcgc
    23281 cgcacggata gcaccatagc ggcgctcaat cttctgtcca tagcgatgga tagcggattt
    23341 gctgatgacg tacccccgct cacgcagcag ggactccagc tcgttatacc cgctgaagcc
    23401 ggattcagtc agcgcccgtt caagccagcg gcgcacgtct tccggcagct tttctattgt
    23461 gctgcgtctg gccattattc actccagtac ttttccgggc gggcaatacc cggaccacat
    23521 tccacggtgt attccaccag gtctacgccg aggcgggtca ggtcagcaaa ccagtcacca
    23581 gaaggttttt tctccagctc aaccatttta cggtcagcca gataatccag ttcgcggcgc
    23641 agctccagcg gtgtggtgtc cgggtagatg gcacgggaca catccagcag cagcgtctcg
    23701 ctggcggtgt aagggcgggt cttgttcagg gcaaccagca gactccagcg cagggattcg
    23761 cggcgtactc gggtaatatc gaccattatt gacctccggt attgcggtac tgctgtacca
    23821 cttccagttt gttataaagc gcgtccagtt tggcctcaat gactgtctgg ccacggatat
    23881 aatcctctcg acggacataa ttcagcggta aatccgcttt aaatcgcata aattcttttt
    23941 ccagctcgcc ccagttggag gcggattgtt gcagggcctg ttcaagggag gcgaatcgtg
    24001 ccgcctggcg ttcttctgtt ttactgaaca accatttggc gagccctccc acaaacccca
    24061 tgaaggtgag cagaaaactc accaccgtcc agaattcaac ctgcagtgtc atttctgtaa
    24121 tccttcccgt tcatccagca gcgcgtttat ctggttccgc cagcgacgac attgccatgc
    24181 gttgtcgatg atgttggcga gaacgtcacg ctgggagaca cccgaatcgc gtaaccgggt
    24241 gtcagtggtt tcaggttgcc cggtcgctgt gccagagcgg gtgccagtgg cggcaactga
    24301 gtctgaatga ccggtgtcga cggatgcgtt gtcatatccg agtgcggcgt tgtactggcg
    24361 cacgaaaccg cgagtaaaca cgcactcaat gggatgactc ttaccttttt catcaatcca
    24421 gcgctgtgtg acatcgttaa tttgcccctg tagttgtttg ttccggctct ccagttgagc
    24481 aatctgctca agataaccgg cttcagcccg ctgcccggcg gccacctgct cctgataccg
    24541 tctggcccaa gcccgcagcg cagcattctc aagcgttgcc tgcgccgttt tgtacgcgtc
    24601 aaatgctgac tgcaactgac tgagcgcggt attaccgtca cgctttgcag agtcatgacc
    24661 acttctgtat cccatggcat acaggccgat cagaaaggca ttgataagaa tggccagcag
    24721 aatgccgcgc cacggcagct ttttaaccag atgccacaca actgctgcct ccccatgtga
    24781 gatatcgcgg tgccagttcg cgcaggatgc gctgcggata atggcggttc tcccgccagc
    24841 tggccgcatt gcgtccggca ttcaccgtgg cgacatgtcc gaaccagcgg gtgctgtcca
    24901 gacctttctg tgatgcaagc cgcctgtccc gttgtaccca gcccagacca ccgttatagc
    24961 ctgacagtgt catggccata cgctcgcagt cgttggcggc gctgacgcgc tgccacagcc
    25021 agcggtcata gctgaccagc gcccggatgg cccatgccgg attaaacggc tcacggctgt
    25081 taagtcccgg catcagctga cttatccagt cggcagtggc aggcatgaac tgcgccagtc
    25141 cctgagcacc agccggcgag accgcatcag gtcgccagcc gctttcctga tgcagttgcg
    25201 cggcgaaatc tgccaccggc gcagacagtc cccattcaag ccgggcatta cggatcacat
    25261 catcgcgata ctgcagcgca gcctgcggag gctgcgctgc gcgggcctga ctgaagaagc
    25321 caccacacca gagcagccag acaatcacca gtttgccagc aagctgccac cagaagctgt
    25381 atttatcgtt acgtggttcg ccgtgtctga tggcggttac gcccagacca aaggcgaaca
    25441 gaataatgag ggtgatttga ggccagttca tggtcacagt cctgttgcca cagccagaca
    25501 gaccgctgca acaatcagtg cacggcggat taacgcagca gaaaatacaa ggtgaaggcc
    25561 ggtctgcaca gggaaacgcc cttcagccat cagcctgtct tcatgtttca ggtactgacc
    25621 gggacgggct ttggggaaga gcgaacggtc aagccagtaa cccagcactg ctgccagcgt
    25681 gatgagtgcc agcttgtaga tcacaacagg caactgctgt ggcgagacca gagcgatggt
    25741 gcccagcaac agcactgagg tcagcagcca gccgctgagg cgaggttttt taacaggggg
    25801 aatgaatttt ttcaggcttt tcatgtgtgt ctccttgttt ggtggagaca gcatcacaaa
    25861 tgtggcggga tacggatttt aaagcgcgtt ataagtgttc gcagagggaa gcgaccagaa
    25921 tggtcgcaca caaaacatag gggaggcaag cacaatgact gaaccgaaac agtctggtat
    25981 taaaaaaggg ttcctctttg ggagcagaat cggagagcag aataaaactt acacgctaag
    26041 cttggatgca tgttgtccaa ccgaagcgat tatagagctg aaaaacgttc tggttgcact
    26101 tgaagagaga agaagtcatt tcgccagcgg agaaaacaac gtcatgctaa ttggcgatgt
    26161 ggcggcgaca tcacgatata aaaccgcacc taatcctgcg attcagttat ccttcgacca
    26221 acgaatactg gaagcggaat cagcagttcc cagaacggtt cgtcctcacc agtcttaact
    26281 ggggctgctt cacccgacca gtaaagccaa gcacggtgat acagttccag caccgggcaa
    26341 tcactgttca ataacctcat atctccgcga tgcacctgct catttgtgat gacttcatat
    26401 atcggtgccg ttcttatttc gtcttcgcct ctctcatcag ccagtaattc cctgaacttc
    26461 ctgacttcac tcagatgctg acaggcaaac agtgactgat agcgggaagg tttatcagga
    26521 aaatggctac ggcgaactaa ctcaaacacc agaccgacca gcagacttct gctgattcca
    26581 ctgtcatctt ccattttagg gccggggtta tagagatagt tatacccgtg actcgaaagg
    26641 ccattgggat acatctgatg aagaaaagcg gccagttctg gcatttcagg ctcgtatttt
    26701 ccggtaatta catgacaagg cgaaggcaga cgactagcgg aatcaagtgt gtaatacgtt
    26761 cccattcaga aaacaatcct tatcgaacaa acactgcacg taggtaatgc atccagtaaa
    26821 tcgcgttaat agaaatcatg tatctcaaaa cttcactctg aactctgaca gtgaaagaac
    26881 gaccagcccg gtacccgaac tccaggctgg tcatcgcccc acaggaatgc actgtgagcc
    26941 gaccgaggtt cagtcagtct cgcgagacca gactagcctg ccatattttt tataattata
    27001 aaagggttac agattatgaa aatgcagaca ttccccatcg ctccatggat tggttgtaaa
    27061 cgccgccatg ccagacatat tctggccgat gtttcctagc acgcacgaat atgaggcaac
    27121 tgacgagaat aaatgatgcg tcagaatgaa ccgtcgacac cgtgctttca ggtttcaaaa
    27181 atctggagaa ggtatatcta tctgaccgac gtttcgtaac cacatctctt gctttgtttc
    27241 tttattggtt cgggcgatat cattagcatt gtatctgcat gttttaaccg caacctcacg
    27301 aactcgtgct ataggagtat ttgtgtagaa tttaagatct tcctctaccg ctcgtatggc
    27361 gataagccct ccactctgaa aaccgctggt tacttttaaa agatcaactc cttggttata
    27421 aacggcatta catacgttag ggaactcatc ttctacagtt ttggggatca tcagcatatc
    27481 aggaagaccc tgtaacacgt tatcttcctt tggcctccat gcccttccca aaaaagctgt
    27541 tataggttgt ggtgtagaaa gaaatgccaa ttctatataa aacaaaccac tgtagtgatc
    27601 gcgtaacgaa tagactagta aatgatcttt tccagtttta cttgcattgc taagttttat
    27661 atcaaatggt tttttctggt acttaagcaa tccatcaact cccgcataat aatgctttga
    27721 aacagcaaca gaaagttgat gtacttggtt cggataatct gttctatatc gtttaattag
    27781 ccccataata gcctcgcagt tattttctat ttcaaaatat aacgatattt actatctgga
    27841 gtgttttgtt ggtcaagtaa tagagctcta catggggcgg tcaaaacacg ggcataacga
    27901 ccatcggggt ttatgttgat gtaacaagtt ttccctttaa caccataaga ttcaggtatt
    27961 attgcctgtg tagacaccgt gaatttaagg gtatgccagc ccgatttttg cttgtagctt
    28021 gaaaccgaat ggaaatcatc tgagtactta tttaaccctg gaaacatgct gcgtaatgct
    28081 gggatggaat cttctattat tttgtcgttg gttttaatgt taaaaacgct ggtttcagta
    28141 gatgctactg ctttagcatt tccccattta ccagcgaaca caatcattcc cacgaaaaat
    28201 attgcgacaa gaactaacgc tccccccaac actactacat gaggcttccg gcttctggtt
    28261 ggtacagtcg cgactgattt tgccccgctg tccgatggag gtgatagagt tttactctca
    28321 tcacgaatat tcagcagatc atcaaaataa ccaaacacag cctgcaactg ctctttgtcc
    28381 aggcctttaa gatgggttgt accgaaatgc ttcagacagt atctgtcacg gtcaagacga
    28441 tacctagggt cagtaagcac cattattttg ctgaccagaa gattacagtc cttcatattc
    28501 tggatctgtt gtgcatatct ctggaggatc tcaaccgcac catgataatc agccgcagtc
    28561 atttcattaa ctgttttggt attaagtttc gtgtggatcg tcatccatag ttcgcgctta
    28621 ctttccccca gatcaaccaa ttcatccatc aatctgtgta aatcgcgttt ttgcagcaac
    28681 gaaattggtt gctgggcaga gggaagtatg tcagcagaat agttatgaat ggtaacatca
    28741 ccgctgacaa cgttacccac atctccgtta actgtttgct tctctttact cattttctct
    28801 ttgtattttt gttcattatt ccacctgttc ctatggttac gttacctttg ataacattcc
    28861 ccagaacatc tccatgaata acctgttccg cccgttctct ggaggagcca gtcgtcaatg
    28921 ccgacaaaac tgaacctttg acttgtaacg gtgcttgccg gtagtgctga agtaactcca
    28981 tttcatcacg tgataaagtt atacctgatg attccccagt aagaatgaac tgtacgttgg
    29041 ccccaattct tgcaaaggct gcaagtaact cacctccggg aactgcaacc cctcgttcat
    29101 atttccccca catttctctt gaaacgccac aaagggctgc tacttcagcc tgcttgagtg
    29161 ataaccgcga acgctcagct ttgaaccgcg acgcgcaaag agaatcaaaa ttcacataaa
    29221 cctcattgac aatgagaaca atagttctca taatctatca catataaaac aaacatcatt
    29281 gcatcaacaa aggagacaac aatgacttcc gaacaagtta aaactctctt ccgtcagcgt
    29341 ggtatcacgt ttacccgctg ggccgaagaa cacggctaca gccgcaatga ggtctatcgt
    29401 gttctcaatg gacagaccaa agctcgttac ggcaaagccc atgaaattgc cgtaaagctg
    29461 ggattaaaac ccacgtcaaa tgcggcataa attttccata tatgcaacag gttatcacat
    29521 attgcaaaaa tggaaatgtg acatgagtaa agtaaatatc tccagttccg ggacccgcat
    29581 cctacgtgta ctcaaagctc tacgcggtca ctctctgaac ggtgtttcta acggtgaact
    29641 ggcatcagcc ctagatgagt ccccggcgaa catcaatcga gcactcaata cccttatcga
    29701 agagggacta gccctgaaac tggaaaacgg gcgtttcgca ccaggcatcc agttgctgca
    29761 gatcgccatg gcccacagta acgagatggc acgtgcacag gatcgcatta acgaaatcaa
    29821 ccaacgtgtt atttcaggta gtcgtttgta aggagtaatc aatgggacgc accaaatcac
    29881 cggttagcac tgaactgaat gtcgaggtac cgctgtcgga tgatctcaat gtcagtctaa
    29941 acgccatgac acagcatcgc atggaaatca tgcaacagtt cggcgatggt ctgccctatg
    30001 agcgcgatcg tgttgtccat gaggcacgct tttatatggc acagagcgct gaggccatgc
    30061 tggaggcagg taagcggctg ataatcctta aggaaaatga accacacggg gagtttataa
    30121 agattcttga aagtgagttg gggctggcat accggacatc tgtgagaatg atgcaagcat
    30181 cgacaaaata cttatccccg acgctgaaac caaatgtgcc aacgttggca catttgggaa
    30241 aagccaaact tttcgaatta atgacagaag acgacgaaga gcttgccgaa ctggccgacg
    30301 gcggcacagt tgccggcatg acgcttgacg atgttgatcg catgtcagta cgtgaattgc
    30361 gtcaggccct gcgtgaagcg cgcgaaacca acgcagcaca acagcgtgtt ctcgccgata
    30421 aaaatgaaaa aatagactca ctctccacca gactggagaa gaaatcccgt attcagccac
    30481 ctgagcctga tgaagaggtg aagaaactgc gggcggaagt gaccgcgtta gcggttgagg
    30541 cggaatctgc catcgccgtt cgactgtcca gcgcttttga gacactgtgc gcatattgta
    30601 ctgaaaacat gattgatacc cccagagact tcatggcagg cctggtctgt caactggaaa
    30661 gcacagcgcg tagcctgcgc tccacatttg acctgccgga cgagccaaca ggcgatgtcg
    30721 cgccttcatg gctgactgag ccgacgccac agattaacgg gctggaggca taaccaatga
    30781 atgctgccct gactgaacga ctggtttatg tcgcccgcgc ggcacgtgac gcggggcatg
    30841 gtaaacgcgg tgcaatatac gacgctgcct gtgctgaact tggcatgtcc cgcgccactc
    30901 tgctgcgcag gctgaaggag gtatctgtga ctgataaacg caaaaaacgc gccgatgccg
    30961 ggcgcagcgc cctgacccgc gacgaagccg cgctgatatc tgccacactg cgtgaggcca
    31021 cccgcaagaa cggtaagcgt ctctattcca tcgcagatgc agtggaaacc ctgcgggcta
    31081 acggctttat ctccgcaggc agaacagatg aaaccaccgg tgagtttttc ccgttgtccg
    31141 aagatgccat cagtcgtgcc ttgcgtaact atggcctgca cccagaacaa ctggatgccc
    31201 ctgcgccgca taccgaagtg gccagtctgc atcccaatca cgtctggcag attgatgcct
    31261 cgctctgcac gctttactac ctgagcaatg gacacaaagg gctgcaggtg atggacagcg
    31321 cgaagttcta caagaacaaa cccgctaacc ttgcccgtat cgccagtgac cgcgtgtgga
    31381 gttacgagat taccgaccat gccagcggct ggatttacgt tgagtatgtg acgggtgcgg
    31441 aatccggtga gaacctgtgt tctgtgctta tcaacgccat gcaggagcgt ggcggcgcag
    31501 acgtgctgca cggcgtgccg aaaatactct atctcgaccc cggctcggca aacaccgcgg
    31561 gtatgacgaa aaacatgtgc cgctcactgg gcatcgacct gatagcgcac aagccgcata
    31621 acgcccgcgc caccggacag gtggaaaagg cgcgtgacat tatcgaacgc aagctggagc
    31681 cgggtctgaa attccggccg gttcacagtc tggaagaact caacgcgctg gccgcgaaat
    31741 ggcgtagcca ctttaacgca acagctgttc acagccgcca cggtaaaacc cgcacggata
    31801 tctggctgaa gattactgct gagcagctga aaaaagcgcc ttccgttgag gtatgccgtg
    31861 aactggctgt ggcgacacca gaactccgca aagtcacgcc aaaacttcgt gtctcgttcc
    31921 ggggcactga atttgacgta tcaacggtac cgggcgtact ggttggtgaa aaactgatga
    31981 ttacccgtaa cccatggcgc agcgatgtgg cacaggtggt tctgaccggt gaggacggcc
    32041 acgagacgtt cttcctggtc gaagaggtca gaaagaacga gtttggcttt gctgaaggcg
    32101 cggcggtatt tggcgaaagt tacaaagccc tgccggaaac accggcacag acggcggcaa
    32161 aagaaaccga agcgctggtc accggtacag acaacgccgc agatgcagct gccgcacgca
    32221 aggcgaaggc gctgccgttc ggcgggcggc ttgacccgta taaacatatt gatgacgcca
    32281 cacttccggc ctgtataccg aagcgcggtc aggtctctga tgtacgcaga ccgcgcactg
    32341 aacaacgtcc catgactcat gtggaggccg cgaaagccct gcgcgataag ttcagcgccg
    32401 acggccttac ctggacgccg gaacattacc gccagttaac ggcacagtac ccggacggcg
    32461 taccggaagc cgcactggat gaagtaatgg ccacgctgac cacgccggcc cgcagcagcg
    32521 ttatcagcat tgttaacggc aactgaggag ggaaacatgc tggtactgaa gcagcaactg
    32581 aaagaggccc gtattccaca ggcggtggtg gcgagagctg tcgatgtttc tgaggccacg
    32641 ctggcccaga ttgtgaatca taacgcgtgg ccccgcacca gccccggaga agtgcgccgg
    32701 cgtcttgcgt cctggctgga aagtcagggg attgatacag cgaagagttt tgatgctgta
    32761 cagggcgcgg ccacgccccg tacagcgggt accacagata aaacgagcct cagtgaggaa
    32821 gagaatatgt tactcaaaaa acaggtgtta ttcccggcaa ccaaaaaagc gtttggtctt
    32881 ttccgtgacc cgttcgccga cgaagccatg cagggttctg atgatgtgtt caccaccccg
    32941 gacattcgct acgtgcgtga ggcgttgtac cagacagccc gtcatggtgg gtttatggcc
    33001 gtcatcggtg agtccggtgc gggtaaatcc acgctgcgcc gcgacctgac tgaacgtatc
    33061 aaccgcgaga atgcgccggt aattgttatc gagccataca tcatcgctat ggaagacaac
    33121 gatgtgaaag ggaaaaccct gaaggcagca gctattgccg aagccattat cagtaccatc
    33181 gcaccactgg aaagcatcag acgcagtcag gacgcccgct ttcgccagtt gcatcgcgtc
    33241 ctgaaagaca gcagccaggc ggggttcagc cacgttctgg tgattgagga ggcccacagt
    33301 ctgcccattc cgacactgaa acacctcaaa cgcttttttg agctggagtc cggtttcaaa
    33361 aaactgctgt ccatcgtgct gattggccag ccggaactgg cgacaaaact gtctgaacgc
    33421 aatatggaag tccgtgaagt cgttcagcgc tgtgaggtgg tcgaacttct gcctctggac
    33481 aataaccttg aagagtttct gacgttcaaa ctgcaacggg caggtaaaca actgacggac
    33541 attatggacg ccagtgcagt ggatgccata cgtgcccgcc tgagcaatcc gggaagtcat
    33601 cgtaaaaata tggtcagcct gttgtatccg ctggccgtca gtaacctggt gatagccgcc
    33661 atgaatctgg ccgctgaaat cggggttcca caggtcaacg ctgacgttgt caaaggggtt
    33721 taataatgaa atccaccaca ggtatcaacc agcaaatcag caaagtgcag tcagccatta
    33781 tggcgcttaa ggcgacgaac acggatgtac aaagcaccac catcaggggt aacaaacctg
    33841 tcatccgcgt ttcccggagt gcgcattgca tgcgcatgct tgagcaggga aaggcctgtt
    33901 atctgtatac cggacatgac cacaggggat atttccgtca gggcgttttc gaactgcacg
    33961 gctgtcgcgt cgtgtggccg gaatctttgt ggtaatcagc acaactggag aaatcataag
    34021 atatggcaaa aagtacaaaa ggggcaaaac gtatcaagtc cgcagcggca ctctgggtac
    34081 cggggacacg cgaagaggtc attgagggaa tcagactgct cggtgacgca caacgtgaac
    34141 tggtcagggc tgaaacagaa atgaatgacg ccattggcga tatcaccgca cgttatgccc
    34201 cgctcaccga gagcctaaaa aaacgcatgg ccgaactgca gtccggtatt cagacatggt
    34261 gtgaggcaca ccgtgatgaa ctgaccggca acgggaaggt gaagttcgct aacctcacca
    34321 ccggcgaggt gcagtggcga aaccgtccgc catcagtcag tatccgtgga gcggataatg
    34381 ttattgaact actgagacgt ctggggcttg agcgctttat tcgtgtaaaa gaggaaataa
    34441 ataaagatgc cattctgaat gaaaaagagg ccgtaaaaaa tattcccggt attaccataa
    34501 gaagtgatat tgaggatttt tcaataattc cttttgagca ggatgtgcag taaacacacc
    34561 acgttaatta tttaataaaa acattttctt ttttattccg gcgtcagcgc cgtgggcttc
    34621 tgcacgcc
//
LOCUS       DAZFRG010000020        34530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFRG010000020 DAZFRG010000000
VERSION     DAZFRG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.020"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..1255)
                     /gene="amiC"
                     /locus_tag="ACHKUC_000773"
     CDS             complement(2..1255)
                     /gene="amiC"
                     /locus_tag="ACHKUC_000773"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO6617071.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1487..2818
                     /gene="argA"
                     /locus_tag="ACHKUC_000774"
     CDS             1487..2818
                     /gene="argA"
                     /locus_tag="ACHKUC_000774"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO6617072.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2880..4706)
                     /gene="recD"
                     /locus_tag="ACHKUC_000775"
     CDS             complement(2880..4706)
                     /gene="recD"
                     /locus_tag="ACHKUC_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO6617073.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTDAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4706..8248)
                     /gene="recB"
                     /locus_tag="ACHKUC_000776"
     CDS             complement(4706..8248)
                     /gene="recB"
                     /locus_tag="ACHKUC_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6617074.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMKGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHLFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCNDDIVWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLIPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8241..11129)
                     /gene="ptrA"
                     /locus_tag="ACHKUC_000777"
     CDS             complement(8241..11129)
                     /gene="ptrA"
                     /locus_tag="ACHKUC_000777"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO6617075.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDTLKSGARPEFMVIGNMTEAQATTLARHVQKKLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11305..14673)
                     /gene="recC"
                     /locus_tag="ACHKUC_000778"
     CDS             complement(11305..14673)
                     /gene="recC"
                     /locus_tag="ACHKUC_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO6617076.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGVGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLSS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14686..15009)
                     /locus_tag="ACHKUC_000779"
     CDS             complement(14686..15009)
                     /locus_tag="ACHKUC_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO6617077.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHSWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(14994..15401)
                     /locus_tag="ACHKUC_000780"
     CDS             complement(14994..15401)
                     /locus_tag="ACHKUC_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO6617078.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQMQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15398..15961)
                     /locus_tag="ACHKUC_000781"
     CDS             complement(15398..15961)
                     /locus_tag="ACHKUC_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6617079.1"
                     /translation="MSVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(15952..16422)
                     /locus_tag="ACHKUC_000782"
     CDS             complement(15952..16422)
                     /locus_tag="ACHKUC_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6617080.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16607..17401)
                     /gene="thyA"
                     /locus_tag="ACHKUC_000783"
     CDS             complement(16607..17401)
                     /gene="thyA"
                     /locus_tag="ACHKUC_000783"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO6617081.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17408..18283)
                     /gene="lgt"
                     /locus_tag="ACHKUC_000784"
     CDS             complement(17408..18283)
                     /gene="lgt"
                     /locus_tag="ACHKUC_000784"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO6617082.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18434..20680)
                     /gene="ptsP"
                     /locus_tag="ACHKUC_000785"
     CDS             complement(18434..20680)
                     /gene="ptsP"
                     /locus_tag="ACHKUC_000785"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6617083.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20693..21223)
                     /gene="rppH"
                     /locus_tag="ACHKUC_000786"
     CDS             complement(20693..21223)
                     /gene="rppH"
                     /locus_tag="ACHKUC_000786"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO6617084.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21576..21722)
                     /gene="ygdT"
                     /locus_tag="ACHKUC_000787"
     CDS             complement(21576..21722)
                     /gene="ygdT"
                     /locus_tag="ACHKUC_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO6617085.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21908..22597
                     /gene="mutH"
                     /locus_tag="ACHKUC_000788"
     CDS             21908..22597
                     /gene="mutH"
                     /locus_tag="ACHKUC_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO6617086.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22666..23379
                     /locus_tag="ACHKUC_000789"
     CDS             22666..23379
                     /locus_tag="ACHKUC_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6617087.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAI
                     FFSIAVESLNLIRNKKNPL"
     gene            23517..23735
                     /gene="ygdR"
                     /locus_tag="ACHKUC_000790"
     CDS             23517..23735
                     /gene="ygdR"
                     /locus_tag="ACHKUC_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO6617088.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23843..24883
                     /locus_tag="ACHKUC_000791"
     CDS             23843..24883
                     /locus_tag="ACHKUC_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO6617089.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24915..26108)
                     /gene="lplT"
                     /locus_tag="ACHKUC_000792"
     CDS             complement(24915..26108)
                     /gene="lplT"
                     /locus_tag="ACHKUC_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO6617090.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26101..28260)
                     /gene="aas"
                     /locus_tag="ACHKUC_000793"
     CDS             complement(26101..28260)
                     /gene="aas"
                     /locus_tag="ACHKUC_000793"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO6617091.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28846..29877
                     /gene="galR"
                     /locus_tag="ACHKUC_000794"
     CDS             28846..29877
                     /gene="galR"
                     /locus_tag="ACHKUC_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO6617092.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29884..31146)
                     /gene="lysA"
                     /locus_tag="ACHKUC_000795"
     CDS             complement(29884..31146)
                     /gene="lysA"
                     /locus_tag="ACHKUC_000795"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO6617093.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31268..32203
                     /gene="lysR"
                     /locus_tag="ACHKUC_000796"
     CDS             31268..32203
                     /gene="lysR"
                     /locus_tag="ACHKUC_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO6617094.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32190..32882)
                     /gene="ygeA"
                     /locus_tag="ACHKUC_000797"
     CDS             complement(32190..32882)
                     /gene="ygeA"
                     /locus_tag="ACHKUC_000797"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO6617095.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRLGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33011..34429)
                     /gene="araE"
                     /locus_tag="ACHKUC_000798"
     CDS             complement(33011..34429)
                     /gene="araE"
                     /locus_tag="ACHKUC_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO6617096.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     RALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKMGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8473 a         9472 c         8539 g         8046 t
ORIGIN      
        1 attatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatt cccgcaagaa
       61 tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt
      121 tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt agtacggcaa
      181 acccggcctg ttcaacttga tttttatgca gcttgttgat tttaccgagc ttattcagca
      241 ccgctttacc aaacttaagg ctgtcggcaa tggtcagcga ctgcaccata tcgaacatgg
      301 tgtggtcgac atagcggtca ccgcttttgc ttacgccacc aatcaagtcc gaggcgttct
      361 gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag agcgcaaaca
      421 ccgatgaacc gctcggctgg cgactggtaa aggcgtcggc atggatagaa acaaacaagt
      481 cagcgcgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
      541 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc agacggcgag
      601 ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt
      661 cgccaccgtg gccagggtca agcataatga caatcggacg atcccgccca gctttacccg
      721 gttgtggacc actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
      781 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca
      841 ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt
      901 gctttaattc aaaaaccata cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact
      961 tgatgaacgg gtcgtcagcg cggatttgcg cagccatccc cttgagcacc gagttcaggt
     1021 ttacatcttc gatatccacc accacgcgtt caggattact caacgcgaac tgcttatatt
     1081 tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc cagacgcgca
     1141 ccgccacgac ctggctgacc gcagccaggc tgacctgact tacgctcaat agccacatgg
     1201 cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
     1261 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg accaatttga ccggaaactt
     1321 tagcgaaaga cgcataatct gtcatctata aaacggtaaa cattcttttt atattcacgg
     1381 tattactgat aaaaaactcg ttcccgcata aaatttacac ttgcaccctg cgaaaaaaca
     1441 gaataaaaat acaataattt cgaataatca tgcaaagagg tgtaccgtgg taaaggaacg
     1501 taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
     1561 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
     1621 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
     1681 acgtccgcag atcgacgcaa atctggctgc acatcaccac gaaccgctgt atcacaagaa
     1741 tatacgtgtg accgacgcca aaacactgga actggtgaag caggcagcgg gaacattgca
     1801 actggatatt actgctcgcc tgtcgatgag tctcaataac acaccgctgc agggcgcgca
     1861 tatcaacgtc gtcagtggta attttattat tgcccagccg ctgggcgtcg atgacggcgt
     1921 ggattactgt catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
     1981 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcggtcactg gcgagagctt
     2041 taatctgacc tcggaagaga ttgccactca actggccatc aaactgaaag ctgaaaaaat
     2101 gattggtttt tgctcttccc agggcgtcac taatgacgac ggtgatattg tctccgaact
     2161 tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg attacaactc
     2221 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
     2281 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
     2341 tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga
     2401 tatcggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg
     2461 ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc gcgataatac
     2521 gactattgcc tgcgcagcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
     2581 tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat
     2641 tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat
     2701 tcactggttc caggaacgtg gatttacccc agtggatatt gatttactgc ccgagagcaa
     2761 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tggggtaagc
     2821 gatgaaaatc gtcggatgcg acatgcgtaa cactcgtgcg tcgcatccgg caattacgtt
     2881 tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcggtgcg cgtggcaatt
     2941 gccgcactta aaatgcgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcact
     3001 gcggtgtaaa ccagctctcg cgttactacc ggcgtgcgtt gactcggtaa aatcaacgcc
     3061 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt cgtttcgtgc
     3121 tctggcaggc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
     3181 cgcgtcccct gcccgcgatc cagcgcaata ccaatatcgc cattaaacaa cccaagcgcg
     3241 ctgtcattac gggcaatcat cactggtcgg ccttcgtacc aacgagagtg cggatgacga
     3301 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
     3361 aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg aatgattaaa
     3421 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc aagagcttcc
     3481 tcaagcatcg caatataatc ttcgccactc tgtaaaagcc gtttttcgat atcagtaaaa
     3541 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac
     3601 tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg
     3661 cgcaaagatg ccgcgtctgt gccagttcct gccggaacgt gagttcccgt caggcggctt
     3721 agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat atcgcccagc
     3781 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
     3841 tgatcgggca aggcgtcgat cagtctcgac atcataggaa gatcgatcat tgacgcttca
     3901 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
     3961 cccggctgcg cacccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt
     4021 tgttcatcgg tcagcggtaa ttgtcgcaaa gccttgccga gagattcggt taagcgcgcg
     4081 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
     4141 attaacgctg ccagtaactt cgctacggtg gtcgttttac cggtgccagg accaccggaa
     4201 atcaccgaga tccgccgtgt cagcgccact gccgccgcaa ctttttgcca gttaatttca
     4261 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
     4321 gcatgattca cttcgttgaa aaagcgtgcc actgtgcgct cgttacacca catgcgattc
     4381 aaataaagac gatcgccaca gaggatcatc ggcgttggtt catctccccg gctgaccgct
     4441 tgtgaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
     4501 gccaacagcg gatgcgatgc ctcgttattt tccagtcgtg aaagcggcaa acaaacgtgt
     4561 ccctctccgg catcatgact taatagtgcc gccgcgaggg tgacggcagg atgttcatct
     4621 cccgccacgg tcagggcaaa ctgcacgtcc agtgggcgta gctgtttgtg ctccacagct
     4681 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
     4741 catccatcag gtcaatcaac ccggcgttgg gtcgggtcgc gtagatccct tgttgcggat
     4801 gttctttatc aacgccacgc aggaacagat aaataacgcc gccaaagtgg cgctcatagt
     4861 cgtaatcagc aatacgatgg cgcagataac ggtgcagcgc cagggtataa agctgatatt
     4921 gcagatcata gcggtgtgcc tgcattgccg ctgccatagc ctgttgggtg taagccgaac
     4981 tgtcttcacc caaccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
     5041 acaccagatc gataaagcct tttaacatgc cacgtacctg catgaactcc agcggcgggc
     5101 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
     5161 gttcgctaat tggcaggtaa aactccatct ccacctgttt atcgcgatcg gaaagctgat
     5221 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcagtca
     5281 acaccggttc ccactgcggt tcaaagccac cgagctccag tttttcctgt acccagttcg
     5341 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
     5401 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
     5461 ctgcggcatc aacatccagc cgtggtatca gatcctgagc gataccgtga ccacgctgct
     5521 gcaaaccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agcgtcctcg
     5581 cattcagttc tgcagttaat gcatcattaa cctgccaggg ctggttatca ccaatttgtg
     5641 ccgtttgcca gacaatatca tcattgcaca acgcttcaat acaggtacga agccctgccg
     5701 catcttgcgg ttctcctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtat
     5761 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
     5821 ccgaacgcgt cagcgccacg taaagtaaac gcagatcttc cgccagacgt tcggcctccg
     5881 cgaggtcgac gctttctggc gcagcattaa gatccagaac ggcctcaaac aagtggcgat
     5941 cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg
     6001 gatattccag cccttttgat ttgtgtatcg tgacaatctg caccagatgt ttatcacttt
     6061 cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
     6121 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
     6181 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
     6241 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
     6301 gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca ttattcagcg
     6361 tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg cgcagggtgt
     6421 tctcacgttc gggcgtcatc accgcctgca acagccaaag catctcctgt gcttccagag
     6481 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc agcgttaagg
     6541 catcgcgcac ctgagcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
     6601 gcaccggacg cgcatcgtcg ccgttcatca gcaacgcttc gccgcgttgc ccggcttgta
     6661 accagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc
     6721 cgcagctttc gcctttcatc agccacattt tcatcgcagg ctgcgtttca cctttaaaca
     6781 caaaacgtaa cgcctgattt ttcccggctg atttcacagg aataaacggt atttcacgaa
     6841 acatgaacgc gtcatcagtc tggctgaaaa gtttgttaac gctgttcacc attcccggtg
     6901 cggaacgcca gttggtatct aaggtgtagt gagcatgaac ttcgctacgc gccttcatat
     6961 aagtgaagat atccgcaccc cggaatgcat aaatggcctg tttcgggtcg ccaatcagca
     7021 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
     7081 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
     7141 atacctcgcc gctttcacta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
     7201 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
     7261 gagtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgttcg atcgcctcaa
     7321 acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctgag
     7381 agaatttttc cagcgactcc ggcaactgat agctgttggt ctcttcttct gcccaggcgc
     7441 tgatcttctc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
     7501 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttacggcat
     7561 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg gggggcggtg
     7621 ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
     7681 gccctttcca ggtttcaaag acgacctggg caatttcacg cggcagcggg tagcagtggc
     7741 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
     7801 gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaagccgt
     7861 gaatagtaaa gactgccgct tcatccatct gccgctcggc taacaacaac cactgcgcgg
     7921 cttgcgcttt atcgtcgatc tcttccagca ggcgtttgta cagtggattg tcggtggttt
     7981 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgacca cgcaattctg
     8041 ccgtagcagc ctcggtaaag gtcaccacca gcagttcttc aacggtcagc gggcggggaa
     8101 aggcggcaga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
     8161 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
     8221 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
     8281 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg
     8341 ttctggctgc cggaaatctg cgacagaata gccatacctt gcggctcgac caccgcctga
     8401 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
     8461 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
     8521 ccgagcgttt gcggtgcctg cagcatctga gtaattaccg cctgctggat ttgcgcaaac
     8581 tcctctggct tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
     8641 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
     8701 cgccctacgc tcattgggaa cgcaaacacg gcatagccca actgttcttc ggtacgcaac
     8761 tgattgtaga accacggctg tacgatctgc cccaacagag aactatacgc tgagctggtg
     8821 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttacca
     8881 gctttttcaa agatgacgga ttgtttttta tcgaccacga catctttgtt acgacaccac
     8941 tctgaaccat cagcgcccaa ctttttttgc acatggcgtg ccagcgttgt tgcctgggct
     9001 tcagtcatgt tgccgataac cataaactct ggtctagccc ctgattttaa ggtgtcgcga
     9061 taggccagca cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag
     9121 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttgccc
     9181 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc
     9241 gtagcggtat agctaaagta gccctccagc agagcctgga acagctgcgg caggcgctga
     9301 gtgtaaccat tggcattaac cataaggccg ttgttagcat tggtggaaaa acttatgcca
     9361 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
     9421 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
     9481 acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac gcgcagattc
     9541 gactcatcaa caatcaactc tggatggtcg tatttcttct ctgacttaat cagcgagaaa
     9601 tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc ggcttttttc
     9661 tgccagtccg cgaaagtttg tgcgctaatt ttatcgacct gatacggcgc atcgacaaag
     9721 taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
     9781 gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc aatattgact
     9841 gcatccagtg tatgctcaac aggaacgcga atcatggtat cagccagcca ttcgacgtaa
     9901 tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt cgccagttca
     9961 tcgaagtatt gtttatcgat ccctttttca cgtaacagat tgaggtagct aaaaatggcc
    10021 gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat
    10081 acgccgctgt tgccgttgac gataggatcg gaattggcgc taataccctc aactaatccc
    10141 tgcttttgca accagtcaga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    10201 aattcatccg ttttactacg gaacttcgct gaattgttat cgatgcgaaa ctcaacgcgc
    10261 agaactttac gcggcaacgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    10321 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    10381 tccgccgcca ttttcgccaa ctccggcaac ggtttattgc tgtaaataac cgccttcatc
    10441 agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgtac cggattgcct
    10501 ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
    10561 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    10621 aattcggcat tcactgcatt acgttcacgt tcggcgtatt tcttgtcgag caagggttct
    10681 gcaatagcgt ccgccaggcg gtctaccgca ccgggcaagg cgtcgttctc aacttccaga
    10741 tagaaagccg tgcgatacgg tgccgtgctg gcattgtgac tgccgccgtg cattttgaga
    10801 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
    10861 aggtaatgtg ccagcccttg gtacgcctca ggatcttcca gcgacccaac gggcaccacc
    10921 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gtaagaccac cataccgtta
    10981 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    11041 atgggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac aaacaacaat
    11101 aatgctttga accagatgct gcggggcatt cacggacctc ataatcaact ttattttctg
    11161 gccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
    11221 cagcctatgt ctatataacc atcagtccgt gactggtgcg catcataaag taagcggatg
    11281 gattgcgcaa tttttataca atactcatga ctgattaaag cgaaacagcg gtaacaggaa
    11341 acgttgcgac tgttcaacga tagcctccat tgtctctggt gttaattgcc gccagagccg
    11401 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    11461 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    11521 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    11581 cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    11641 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    11701 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    11761 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat
    11821 ctgcacaccg ttgcaggcga gatcaatctc catactctgc cctggctggc gacaggcaat
    11881 gactctgtcg gcaagttgct gcatctcctg acactgcgtt tcccagaaaa tttcaccaaa
    11941 ggcaccatac ggcaaatcac ctgccgcccg gaatcggcgg aacaagcgtt cggcatcatc
    12001 ctgctcaacc agtacattta ataactgctg gttgatttga taacgactaa gcccttcaag
    12061 aataaaaggt tcagtgtcgg gaatttcgct gtcttcggta cggaagttca cctgcaaacg
    12121 catctggaaa aacgcccgta ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    12181 ggtttccggt aaggtaaatg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    12241 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
    12301 tggatcaaac ggcattcggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
    12361 ttcatcacag ttgagcgctt catcgcccgg tagataatgg ctttgcccga tgtagtcgat
    12421 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
    12481 atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca gatagcggtc
    12541 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcagat caaagcccaa
    12601 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    12661 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
    12721 gctgatacgt tcttgatcca ggcgctgtgc cagttcatca cgcaatagtg acagcggcac
    12781 cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    12841 cagcgtcatc gctgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    12901 acaaaccggc aaccactctt ccagcggacg ctcctgcgcc agcccgcgac gccagatgtt
    12961 tagctgcatt agcagtgaag acagatgccc caccagttct gcaattaagc cgctcgattc
    13021 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
    13081 catgcgcgtc aggccaaacc gccaggtgtg ttgaccggta gcgggaagtt ccagctcgcg
    13141 aacgttgtcg tcatctatcc cccaacgaat gccggactcg ttgacccact ggcgtaaata
    13201 acgcagccct tcttcggtga tgtcaaatcg cgctgccagc accggcacat ccagtaatgc
    13261 cagcacgtct tccgacacaa agcggctgtc tggcagcgat aacaggctga taaacgcttc
    13321 aagtacagga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    13381 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    13441 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttcc tccagcatcg ccagcaggcg
    13501 atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    13561 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    13621 agcaacggcg cggttttcca gttccagaat gtcagactga atattatgca gcaggttatc
    13681 tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag aaaggagata
    13741 aatgtagtcg cgcccaagct tgccccatga agccagcagc gggttgccga catcctgttc
    13801 accatcgctg ttaaagagct gcccggcatt ttcgctgtcg cgaaatagcg gtaattcgcg
    13861 atcttcaaaa ctgtgtcggc gctggcgagt cagtagtttc gccagataag cgggatcttt
    13921 aatgtcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    13981 acccagcgcc tgtagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    14041 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    14101 ctgatagaga ttggcgcggt gccagcgcgg ttgcccaagt tcgtcggtat attccaccaa
    14161 cgccttccac aacggcgctt gccaggcctg tgcttctccc accccttcta ccagatgtcc
    14221 tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa acaggtccgc
    14281 cgcttttgaa gaaagctgga acagttttcg cttgtcacta tcgtcagtca gataatgccg
    14341 caacagggta aagtcttcgc gctccaacag ttgcggcagc agagtcatca gtttccagct
    14401 catgctctgt ttgttaaaag cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    14461 atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    14521 cgacagggtc atttgcagcc actgtgccat accggtactt tgcaccagaa tcatctctgg
    14581 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    14641 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    14701 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    14761 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttag caggtggcga
    14821 aatcgcgcgc agttgcgttt gctgccagct atgccgccag agctgttggt actggtttct
    14881 gctggcaaga ctgttcatta atgttcgctg gaaacccgat aacgcagtga caatcatcac
    14941 catcaacacc atcgccaaca ttacctccgg cagactaaag ccttgttgat tcctcaggga
    15001 agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa
    15061 acaatgtttc catcgatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca
    15121 atcaataagg cttcattatc tgccagtaaa cgcagacaaa cccgggcacc ggtggcagca
    15181 tacagtaagc actgaactgc gggctgcatc tgccaggagt gcatttttcc ccactccagc
    15241 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaagca
    15301 aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaat cagcagcatc
    15361 aggaccagag ccagtgacga aacacccttt tcgcggttca caggttggat cctgtcacgc
    15421 tatagctggc atccaccacg gtttgcggat cagccttgct ggcagcgtgc atattaaccg
    15481 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    15541 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    15601 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct tttaccggtt
    15661 cgcgatccca gataccatta ctgttcgcat cccactgcac aatgacacag tcaccctgtc
    15721 cgacaatttc cagcccttcg cccgtacagc tgccatggca ataacccgcc ctctggagat
    15781 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgt
    15841 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    15901 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacagaca
    15961 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca
    16021 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    16081 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gcggcacaaa
    16141 gaccaatggt gaactgccat gacaggtatt cgccccagca acggaactca caaggcacca
    16201 taacgtcccc tccctgataa gactgatact gtggtcgcgg ttatgccagt tggcatcttc
    16261 acgtaaatag agcaaatagt cccgcgcctg gctggcagtt tgccaaagcc gttgcgactg
    16321 ctgccagtat tgccagccat agagcccact tgcgcttagc atgaccagaa tcagcatcgc
    16381 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    16441 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    16501 ccgttgcagg acgttgcaaa atttcgggaa ggtgtctcga agaatttaac ggagggcaaa
    16561 aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg
    16621 gcgctttaat gcccggatgt ggatcgtatc cttcaatctc aaagtcttcg aaacggtagt
    16681 cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc
    16741 ttaattgcag atgagtttga tccatatggt tgctgtagag atgcgtgtcg ccaccggtcc
    16801 agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc agtaacgcgt
    16861 agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga taaagctggc
    16921 aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt gccagcgcca
    16981 ttttatccag ttcgcctacg ttccacgctg aaacaataat gcgacgggaa tccgggtcgt
    17041 ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc ggcgttggcc
    17101 aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg gcccattcgt
    17161 cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcaggaacc
    17221 acagcagttc gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc
    17281 catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac
    17341 ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca
    17401 tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca
    17461 cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg
    17521 caccagtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt
    17581 aaccaatcag gaacaagcct gagacagctc ccattgggcg cggtttacga atatacaggt
    17641 tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc
    17701 gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca
    17761 aaatatcttc tgtacgggag ccagggaaca gcatggcaaa cgggaagttc gggtcaacgc
    17821 ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa
    17881 tgagtggggc aataaaatca gagacctgga agaaggaacg tttagtacgg cgggcgaaga
    17941 taatcatcac cacgataacg ccaatcaggc cgccgtggaa ggacatgccg ccgtcccaga
    18001 cacggaaaag atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac
    18061 caatacgtcc accgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat
    18121 ttttggtcca gccactgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa
    18181 tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa
    18241 tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct
    18301 tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca
    18361 aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg
    18421 tatatgatcc gcgctataac cccccgcgaa tcagcccgcc catgccgcga cgctccataa
    18481 aggcagcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa
    18541 gattttctgc ttcagcaaaa tcaatgcgcc gcagcaggta ttttacgcgc gctacagaac
    18601 gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat
    18661 cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga
    18721 tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg
    18781 tattgttgcg atcaacggcc aggatgtatt gcgtcagatc gttggtgcca acagagatga
    18841 aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga
    18901 tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac
    18961 gttcaatcag gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc ggcaacagaa
    19021 tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga
    19081 tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc
    19141 tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg
    19201 gtttatcatt aaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg
    19261 gaaaaccact ttgcagcatg aatgggattt cagtgcgata aagtcctatg ccatcaatac
    19321 ggctgcccag tttttcttca tgttccgggc ttaaaccggc attgagcatg actttaatgc
    19381 gttcgccgct ttttaactgg gcgggtaaat tgacgtcatc ttccgccaga cggctaagct
    19441 cgatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca
    19501 gcaattcacc gcgataacca tcgacgatca gcgtccgacg atgcagtacc gaaggctgaa
    19561 tatccgcgcc catcacggta ggtatcccca gcgcacgtac catgatcgca gcatgggagt
    19621 tggcagcgcc atcgcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagcg
    19681 ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt
    19741 gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc
    19801 gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg
    19861 tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca
    19921 gccgggtatc cgaaagcagg tgtgagtaaa gatcgaaaat agccgccgtt tctttttgtg
    19981 caccggcagc aaagcgtttg ctatagcggc gaaactcgtt agccgcctct tccaacgccc
    20041 cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca
    20101 ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta
    20161 atgcacggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa
    20221 gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact
    20281 ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg
    20341 gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg
    20401 ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga
    20461 tcccttcatc aaacgcgagc gttacagtgc gaccacgtgg ttttttcagt ccccgggtcg
    20521 ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat
    20581 ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc
    20641 tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc
    20701 ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca
    20761 ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct
    20821 gtctgactgg ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg
    20881 tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt
    20941 ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca
    21001 accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa
    21061 acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa
    21121 attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa
    21181 tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata
    21241 aagctttata tacgaatgat tgtttcatac tccagtaaga cggtaaacca ctctctgcag
    21301 ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag
    21361 gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg
    21421 gaattactac gggaaaaccc ggagcattct gaataagaca gatatagaga ttttaaaata
    21481 ttcattatca gtcaatgcat tacatgtgat ttgagtaata aaaaagagta attcgtgacc
    21541 caggtcacac ctctcattta cgtgttggcg aaagatcaac caatgccgta tttatccaca
    21601 gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag
    21661 gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac
    21721 atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt gcagtgttgc
    21781 gcaactcgtt taccggcaac ctaaaaagca atataaatca gagaattgaa caacgcatgt
    21841 ggaaaaagtt acactgcgaa tattcagcac ataattgctg tttgtttttt aaccaaggta
    21901 tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag aacaattgtt
    21961 agcgcaagca caacaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct
    22021 ggttacgccg gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg
    22081 gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact
    22141 taaaactatc cctgtggata gtcttggtcg tccactggaa acaacattcg tttgtgttgc
    22201 cccgttaacg ggcaatagcg gggtgacctg ggaaaccagc cacgtgcgcc acaagctcaa
    22261 acgcgtgctg tggataccgg ttgaaggcga gcgcagcatc ccgctggcaa agcgtcgcgt
    22321 aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagttac gcgaagactg
    22381 ggaagaatta atggatatga ttgttctcgg tcaaattgag cggatcaccg ctcgtcacgg
    22441 ggaatattta cagatacgac cgaaagcagc gaatgcgaaa gcgctgaccg aagctattgg
    22501 tgcccggggc gaacggattc tgacgctgcc gcgcggattt tatttgaaga agaatttcac
    22561 cagtgcgcta ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt
    22621 ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg
    22681 ataaccgatc ctaacgcctg gcttgcgctc ggtacactga cgctgctgga gatcgttctt
    22741 gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt
    22801 gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca
    22861 tccatcgcct gggttacgcg cctgacgaat ccgcttttta caatattcag tcaggaaatt
    22921 tccgcccgtg atttgatcct gcttctgggt ggcttgttcc ttatctggaa agccagcaag
    22981 gaaatccacg aatccatcga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc
    23041 ctcggcgcga tcgtgcagat tatgctgctg gatattatct tcagcctcga ctcggtgatt
    23101 accgctgtgg gtctgtcaga tcacctgttt attatgatgg ctgccgtggt gattgccgta
    23161 ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca tccttcggta
    23221 aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc
    23281 gacatccacg taccgaaagg ttacatctac ttcgcgatat tcttctctat tgcggttgaa
    23341 agcctcaacc tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg
    23401 ggagggcgta ctcactcctg ttaccctcac agtttaagat tttcctgctt tcaagatata
    23461 taacgtcggt ttataaacag actattatca taggtgggcc aaatgaggat aaccggatga
    23521 aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca
    23581 gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa
    23641 ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta
    23701 accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc
    23761 cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg
    23821 tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt
    23881 cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca
    23941 cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta
    24001 cccagtacct ccgcgccccg aaactcaagg gctaaccgaa acctacgtcg gcaactggct
    24061 ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg
    24121 caataatgac aaaggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc
    24181 gctgcatgac agcctcaagc gtctgcagac tgattacctc gatctctatc aggtgcactg
    24241 gccgcagcgc ccaaccaact gcttcggcaa actcggttat agctggacag attctgcgcc
    24301 tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat
    24361 tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc
    24421 ggacaaacac gatctgccgc gtattgtcac cattcagaac ccctacagcc tgttaaaccg
    24481 cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta
    24541 ttcgtgcctg ggtttcggca cactgaccgg gaaatatctc aatggtgcaa aacccgctgg
    24601 cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc
    24661 cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc agatggcgct
    24721 cgcatttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga
    24781 gcagctgaaa actaacgtcg aaagtttgca tctggagttg agcgaagacg tattagctga
    24841 aattgaagcg gtgcatcagg tttatacgta tcctgcacca taagattaac gccggttaaa
    24901 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
    24961 aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat tactgccagc
    25021 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
    25081 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
    25141 acaaaaaagc cccccagcac gccaatcagc atcaacaaag cataggctgg cagcagctcg
    25201 tgttgcaggg agaaaatcag taccactacg ccaatcaaaa tcccggctgg catacagcgt
    25261 gacacggttt ccagcgtaac taacttcgct gccgcacctg caccaaccac gataccaatc
    25321 gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatacccag cgccaccggt
    25381 acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc
    25441 accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg
    25501 cgggtcatgc tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg
    25561 tagatattgg caacgaccgc gccaccgtag gccagtgcgc atgcggccag ggcaaccaga
    25621 atatgccagt cagccagaac accaccagct acggaaccga gcaaaatcgc cgctatggta
    25681 gacgcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa ttcgccgaga
    25741 ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccaacgt atagccgaga
    25801 aacggattga taccaaagca gatactggct gcgcccagta gcttcaggcc gttggcaaac
    25861 atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga
    25921 atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc
    25981 gctttcagta acgccagagt ggcaaacagt agcgcattat cgccaaacgc agagagaaac
    26041 tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac
    26101 tcactcatcg tgttgttccg cttcgtctac ccagcttttc aaagtgacaa agtcaggttt
    26161 gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc
    26221 cggcacgccg tgctcgcggg catactgttg taacttatcg cgcgtcagtt cgttatctgt
    26281 ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac
    26341 tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcacc
    26401 tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa
    26461 acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc
    26521 ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg
    26581 ccctttcagt tgcaggcgtc cgccctcttc gataccagga accgacaaca ggcgcgcgtc
    26641 catacctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga
    26701 aacgacaggc gcgcactcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc
    26761 ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag
    26821 acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc
    26881 gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt aatgcagcgg
    26941 gcttggataa aggaacactt ctgcacctgt gagcagtggc gtaaacaggc ctaccgtcag
    27001 cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc
    27061 aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt
    27121 cggatggcct tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg gctgctgttt
    27181 tacctgtgcc agacgcggca tcagcaaatg agcgaaaatc cacactttgt cggcagtggt
    27241 gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag
    27301 atgccagagt ttgcctttat cgagaaactg acgggaagtg aagatggttt tgatttcagc
    27361 cgccgtaata gcactggtca gcccttttac cccggcggtg tagttcatca ttgcgggaat
    27421 gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat
    27481 taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt
    27541 tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt
    27601 cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt cacgcgggcg
    27661 caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg
    27721 gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa tatgcagagt
    27781 aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc
    27841 cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaatcccgc
    27901 gccatcgtag attttcatca gcgagccagt ggtggtgatg cgtccttcag ggaaaatcac
    27961 cactggtcgt ccctgttcca ccagacgtac cagatgttta atcgccatag gttgcgtcgg
    28021 gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat
    28081 tgaggtgtaa acggcaaata ccggacgtac gggtaaaaac aatgccagca gtatgccatc
    28141 aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg ccttggtgtc
    28201 acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat
    28261 tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag
    28321 aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc
    28381 aagagactgg gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt
    28441 gattgtggtc ttcacaacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat
    28501 gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccgaca aaaaaaaacc
    28561 tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc
    28621 aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc
    28681 aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt
    28741 gcgtttggct cacattccca caatgaaaac acgccgcccc ttgaaccaac gggcgttttc
    28801 cgtaacactg aaataatgta agcgtttacc cactaaggta ttttcatggc gaccataaag
    28861 gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgtgtcat taataattca
    28921 cccaaagcca gcgaagcttc ccggcttgct gtgcatagtg caatggagtc tcttagctat
    28981 cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacgat cggtctggtc
    29041 gttggtgatg tttccgatcc gtttttcggc gcaatggtga aagcggtcga acaggtggct
    29101 tatcacactg gtaatttttt attgattggc aacggttacc acaacgaaca aaaagagcgt
    29161 caggccattg agcaactgat ccgccatcgc tgtgctgcgc tggttgtcca tgccaaaatg
    29221 atcccggatg ccgatttagc ctcgttaatg aaacaaatgc ccggtatggt gctgatcaac
    29281 cgtatcctgc ctggctttga aaaccgttgt attgctctgg acgatcgtta cggtgcctgg
    29341 ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac
    29401 cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt
    29461 ggtattccgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa
    29521 caggcaatga ccgagctttt gggacgaggc agaaatttca ctgcggtagc ctgttataac
    29581 gattcaatgg cggcgggcgc gatgggcgtg ctcaatgata atggtattga tgtaccgggt
    29641 gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg
    29701 accaccgtgc gttacccaat cgtgacgatg gcgacgcagg ctgccgaact ggctttggcg
    29761 ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt
    29821 cgccattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg
    29881 cagttaaagc aattccagcg ccagtaattc ttcaatggtc tggcgacggc gaatcaaccg
    29941 cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt agttggatga
    30001 cattgatgcg ccatatgccc ccgtatcatg cagtaccaga taatcacccg ccttcacttc
    30061 cggcaaggcg cgggtttcga catttccccc ttcctgctgg gtaaagacat cgcccgattc
    30121 acataacggc ccggcgacga cggtttccac cgttggtgcg tgttccagag aacgaccatc
    30181 agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt
    30241 gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacttg
    30301 agtaattaat acgccagcct gcgctaccag aaagcgaccc ggttcaattt ccagtttcac
    30361 aggatggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac cataataatg
    30421 ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga
    30481 aatagcctgt aaatcctgac cgaactcgag gacctgacgc accatagcac cacatacctg
    30541 ttccagatgg gcataatcga cgccagaacc aatgtgcatg tgaatgccga ccagctgtag
    30601 atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt
    30661 gctgttttcg ccaccggtat tggttttttg gctatgcccg tgaccaaacc ccggattaac
    30721 acgcagccat acccgatgcc ctggcgaaac ctgacccagt tggtcgagca tatcaacaga
    30781 acccgcattc accggaattt gcaattcact gactcgttca agcgtcgcct gatcgataac
    30841 atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccagtgc
    30901 acgctctatt tcgcctaacg agacggaatc cacttttacg ccctgctcac gcattaagcg
    30961 caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag
    31021 cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc
    31081 tgcgggcaaa cgcagcagat tttcggcggt gagatcggta tcagtgctga acagtgaatg
    31141 tggcataaca aactccagat aagtgctttt ttatgattac gccacatcat aaaaagaata
    31201 aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag
    31261 agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc
    31321 cggaagcctg actgaggcgg cacacctgct acacacctca cagccaaccg tcagccgcga
    31381 actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcatac gtggacgatt
    31441 acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact
    31501 ggatcgcatt gtcagtgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat
    31561 tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc
    31621 acgttatccc gatgtcagct taaacatcgt gccccaggaa tcaccgctac ttgaagagtg
    31681 gctctcggcc cagcgtcatg atttaggact cactgaaacg ctccatacgc ctgcgggaac
    31741 agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg gccatccgct
    31801 ggcggtaaaa aaggtattaa cgccggatga ttttcacggt gagaactaca tcagcctttc
    31861 ccgtactgac agctatcgcc agttgctgga tcaattgttt actgagaatc aggttaaacg
    31921 acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt
    31981 aggcgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt
    32041 gcggcggttc agcattgcgg ttccattcac cgtcagcctg atccgccccc tgcaccgccc
    32101 gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcgggattac cgaaactggt
    32161 cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg
    32221 gcatggatcg ccgcggtatc aaacacaggc agaacactgc gctcttctgg caccagtaaa
    32281 ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc
    32341 acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa
    32401 ataatctgat taatttttgc ccgttcatcc gcttcaggaa taagacagtt gatggaaaat
    32461 tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgtgt acccagcagc
    32521 gccacacgag tcattcctgc cccggtaatt gcacgtccag tggcatccgc tatgtgtaag
    32581 aaaggcagag agcaacgaga ctcaatggca tccgccactt tgtgcatcgt attggtgcat
    32641 aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatatcc
    32701 ccggttttgt cccactcacc gagacgctgg cactcttcta tttcatgaaa atcgacgcta
    32761 tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc
    32821 agacgatagt aaggaatagt ggattcccag ctcattcctc ccagcaaacc aattgttttc
    32881 attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc
    32941 tcaacggcca ggtgcctaat ctctattaac gaaaaaaggg ccggatgtac agcacatccg
    33001 gcccgtgaaa tcagacgccg atatttctca acttttcgcc tgccatcagt ttgcgctcga
    33061 tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg
    33121 caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta tcaagcagtg
    33181 tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtt gagcaggtga
    33241 tgccaaaatc gcggcatttc agcggctgaa tttcagagca caagatccac accactggcg
    33301 cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca
    33361 agccactgga agccgtaccg ttatcaaact gcatcaggca gtaacccagc accagagtgc
    33421 ctaacgccat cacgctgaaa cccattttca gagccggctt acgccctgct ttatctaccg
    33481 taaagaccgc aatgaaggta gcgaacataa aggtcagccc cacgaccaga gtcgcaatca
    33541 tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgtggcgcg tagtacatga
    33601 tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga aatacagcac
    33661 gacggacgtt acggttgatc ttaaacaatg cccaaccgcc ctgttttaac ttcaggcttt
    33721 cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaata
    33781 cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga
    33841 agaccaccag aataatcagc agaactgctg gtaaagcaag aacccccaac attgcgcgcc
    33901 agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagagtga
    33961 ccatcaactg gtacatgctg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa
    34021 gatatagagg agcggtgtaa gacgcgatcc cgacagcaat gcccaacacc acacgagcgg
    34081 cgattaacat ctctacgctg gtcgcaaaag cggaccctat cgaaccgagt acaaacagga
    34141 tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca
    34201 gcgcaccaat tgctgcgccg agcatcatgc tactgaccac ccattcctgc aaacgactgg
    34261 tcagcacaaa gtgatcggta atgaacggca acgctcgggc gattacgccg atatcaagac
    34321 caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat
    34381 cccgcagagg acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc
    34441 agcagagagt aagacatagt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga
    34501 aatagcataa atatggatta aattgctacg
//
LOCUS       DAZFRG010000021        34220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFRG010000021 DAZFRG010000000
VERSION     DAZFRG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.021"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1577
                     /gene="yqiK"
                     /locus_tag="ACHKUC_000799"
     CDS             <1..1577
                     /gene="yqiK"
                     /locus_tag="ACHKUC_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6617097.1"
                     /translation="ARLYRRASAEQAFVRTGLSGQKVVMSGGAIVMPIFHEIIPINMN
                     TLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIATAAQTLGQRTLSPEDLRM
                     LVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAEDLSKNGLELESVSLTNFNQ
                     TSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEVAVREKNRDALSRKLEIEQ
                     QEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTRILAERQIQETEIDREQAVR
                     SRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANLALAEAVSAQQ
                     NVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEAT
                     RKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKAIDGIKII
                     QVDGLNRGGATGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAA
                     LTSSLSSTTPVEEKAE"
     gene            1817..1876
                     /gene="ibsE"
                     /locus_tag="ACHKUC_000800"
     CDS             1817..1876
                     /gene="ibsE"
                     /locus_tag="ACHKUC_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO6617098.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1996..3429)
                     /gene="hldE"
                     /locus_tag="ACHKUC_000801"
     CDS             complement(1996..3429)
                     /gene="hldE"
                     /locus_tag="ACHKUC_000801"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO6617099.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(3477..6317)
                     /gene="glnE"
                     /locus_tag="ACHKUC_000802"
     CDS             complement(3477..6317)
                     /gene="glnE"
                     /locus_tag="ACHKUC_000802"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO6617100.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRALLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELTRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6340..7641)
                     /gene="ygiF"
                     /locus_tag="ACHKUC_000803"
     CDS             complement(6340..7641)
                     /gene="ygiF"
                     /locus_tag="ACHKUC_000803"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO6617101.1"
                     /translation="MAQEIELKFIVNHSAVAALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEVALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7883..8503
                     /gene="ygiM"
                     /locus_tag="ACHKUC_000804"
     CDS             7883..8503
                     /gene="ygiM"
                     /locus_tag="ACHKUC_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO6617102.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8567..9805
                     /gene="cca"
                     /locus_tag="ACHKUC_000805"
     CDS             8567..9805
                     /gene="cca"
                     /locus_tag="ACHKUC_000805"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO6617103.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(9993..10814)
                     /gene="bacA"
                     /locus_tag="ACHKUC_000806"
     CDS             complement(9993..10814)
                     /gene="bacA"
                     /locus_tag="ACHKUC_000806"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6617104.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10905..11273)
                     /gene="folB"
                     /locus_tag="ACHKUC_000807"
     CDS             complement(10905..11273)
                     /gene="folB"
                     /locus_tag="ACHKUC_000807"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO6617105.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11378..11995
                     /gene="plsY"
                     /locus_tag="ACHKUC_000808"
     CDS             11378..11995
                     /gene="plsY"
                     /locus_tag="ACHKUC_000808"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO6617106.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12008..12940)
                     /gene="ttdR"
                     /locus_tag="ACHKUC_000809"
                     /gene_synonym="dan"
     CDS             complement(12008..12940)
                     /gene="ttdR"
                     /locus_tag="ACHKUC_000809"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO6617107.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13147..14058
                     /gene="ttdA"
                     /locus_tag="ACHKUC_000810"
     CDS             13147..14058
                     /gene="ttdA"
                     /locus_tag="ACHKUC_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO6617108.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            14055..14660
                     /gene="ttdB"
                     /locus_tag="ACHKUC_000811"
     CDS             14055..14660
                     /gene="ttdB"
                     /locus_tag="ACHKUC_000811"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO6617109.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14708..16171
                     /gene="ttdT"
                     /locus_tag="ACHKUC_000812"
     CDS             14708..16171
                     /gene="ttdT"
                     /locus_tag="ACHKUC_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO6617110.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASNATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPILKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIICWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPMVLL"
     gene            complement(16214..17227)
                     /gene="tsaD"
                     /locus_tag="ACHKUC_000813"
     CDS             complement(16214..17227)
                     /gene="tsaD"
                     /locus_tag="ACHKUC_000813"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO6617111.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLIGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17465..17680
                     /gene="rpsU"
                     /locus_tag="ACHKUC_000814"
     CDS             17465..17680
                     /gene="rpsU"
                     /locus_tag="ACHKUC_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO6617112.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17790..19535
                     /gene="dnaG"
                     /locus_tag="ACHKUC_000815"
     CDS             17790..19535
                     /gene="dnaG"
                     /locus_tag="ACHKUC_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO6617113.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADRNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19730..21571
                     /gene="rpoD"
                     /locus_tag="ACHKUC_000816"
     CDS             19730..21571
                     /gene="rpoD"
                     /locus_tag="ACHKUC_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO6617114.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21649..22155)
                     /gene="mug"
                     /locus_tag="ACHKUC_000817"
     CDS             complement(21649..22155)
                     /gene="mug"
                     /locus_tag="ACHKUC_000817"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO6617115.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22280..22355
                     /locus_tag="ACHKUC_000818"
     tRNA            22280..22355
                     /locus_tag="ACHKUC_000818"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22313..22315,aa:Ile,seq:cat)
     gene            complement(22409..23173)
                     /gene="nfeF"
                     /locus_tag="ACHKUC_000819"
                     /gene_synonym="yqjH"
     CDS             complement(22409..23173)
                     /gene="nfeF"
                     /locus_tag="ACHKUC_000819"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO6617116.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELHHELAID
                     FFIHEGGVASSWAMQAQSGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            23461..24084
                     /gene="nfeR"
                     /locus_tag="ACHKUC_000820"
                     /gene_synonym="yqjI"
     CDS             23461..24084
                     /gene="nfeR"
                     /locus_tag="ACHKUC_000820"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO6617117.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24191..25711)
                     /gene="aer"
                     /locus_tag="ACHKUC_000821"
     CDS             complement(24191..25711)
                     /gene="aer"
                     /locus_tag="ACHKUC_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO6617118.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKIRGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVACQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            26129..27508
                     /gene="ygjG"
                     /locus_tag="ACHKUC_000822"
     CDS             26129..27508
                     /gene="ygjG"
                     /locus_tag="ACHKUC_000822"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO6617119.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(27550..27882)
                     /gene="ygjH"
                     /locus_tag="ACHKUC_000823"
     CDS             complement(27550..27882)
                     /gene="ygjH"
                     /locus_tag="ACHKUC_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO6617120.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            28101..29084
                     /gene="ebgR"
                     /locus_tag="ACHKUC_000824"
     CDS             28101..29084
                     /gene="ebgR"
                     /locus_tag="ACHKUC_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO6617121.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29268..32360
                     /gene="ebgA"
                     /locus_tag="ACHKUC_000825"
     CDS             29268..32360
                     /gene="ebgA"
                     /locus_tag="ACHKUC_000825"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO6617122.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVVEQ
                     SDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            32357..32806
                     /gene="ebgC"
                     /locus_tag="ACHKUC_000826"
     CDS             32357..32806
                     /gene="ebgC"
                     /locus_tag="ACHKUC_000826"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO6617123.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            32869..>34220
                     /gene="ygjI"
                     /locus_tag="ACHKUC_000827"
     CDS             32869..>34220
                     /gene="ygjI"
                     /locus_tag="ACHKUC_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6617124.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     "
BASE COUNT         8216 a         9325 c         8723 g         7956 t
ORIGIN      
        1 tcgccaggct ctatcgtcgc gcttcggcag agcaagcatt tgttcgtact ggtttaagtg
       61 ggcaaaaagt ggtgatgagc ggtggcgcaa tcgtgatgcc gatctttcat gaaataattc
      121 ccatcaatat gaataccctg aagctggaag tcagccgttc tactattgat agcctgatta
      181 cgaaagatcg tatgcgtgtt gatgttgtag tggctttctt tgtgcgggta aaaccttcag
      241 tagaagggat tgccaccgcc gcccagacgc tggggcaacg caccctgtcg cctgaagact
      301 tacgtatgct ggtggaagat aaatttgtcg atgccctccg tgcaacagcg gcacaaatga
      361 ccatgcatga gttacaggat acccgcgaga acttcgtgca gggagtacaa aataccgtag
      421 ctgaagatct gtcgaaaaat ggcctggaac tggaaagcgt ttcacttacc aactttaacc
      481 agacctcgaa agaacatttc aatccgaaca atgcctttga cgccgaaggt ttaaccaaac
      541 tgactcagga gacggagcgc cgtcgtcgcg aacgtaacga agttgaacag gatgtggaag
      601 tcgcagtgcg tgagaaaaac cgtgatgcac tttcgcgcaa gttggagatt gaacaacaag
      661 aagcgtttat gacgcttgag caggagcagc aggttaaaac ccgtaccgct gagcagaatg
      721 cgaaaattgc ggcttttgaa gctgaacgtc gtcgtgaagc agagcagacg cgaattctgg
      781 ctgaacgaca gattcaggaa acggaaatcg accgcgaaca ggccgtccgc tcaagaaagg
      841 ttgaggccga acgtgaagtt cgcattaaag agatcgaaca gcagcaggtc accgaaatcg
      901 ctaaccagac gaaatcgatc gctattgccg ccaaatcgga acaacagtcc caggcagaag
      961 cgcgtgctaa ccttgcactc gcagaagcag taagcgccca acaaaacgta gaaaccactc
     1021 gccagactgc cgaagccgat cgtgctaaac aagtggcgtt gatcgccgca gcgcaggatg
     1081 cagaaaccaa agcggttgaa ctgaccgtgc gggcgaaagc agaaaaagaa gccgcagaga
     1141 tgcaggcggc ggctatcgtt gagttagccg aagctacacg taaaaagggt ctggcggaag
     1201 cagaagcaca acgtgcgctg aacgatgcta tcaacgtact ttctgatgaa caaaccagcc
     1261 ttaaattcaa actggcatta ttgcaggcgc tgcctgcggt aattgagaaa tccgttgagc
     1321 ctatgaaggc tatcgacggc atcaaaatta ttcaggtcga tgggctgaat cgtggtggcg
     1381 ctacgggtga tgcaaacacg gggaatgtgg ggggcggaaa cctggcagaa caagcgttat
     1441 cagccgcact ctcttaccgc acacaggcac cgctgattga ctccttgctc aatgaaattg
     1501 gcgtttcagg cggctcactg gcggcattga cttcatcctt aagctcaaca actcccgtcg
     1561 aagaaaaagc agagtaaacc ctcttctatg aataatccct ggcccaaaaa gccggggatt
     1621 ttttatatct gcgttccgct aaaaggtgca aatgatcagg ctgttgcagc attttgcgtg
     1681 actgctcggg gaaggcaaaa ctgcctctgg gaaagcgttg cgcggagtcc ggcgctcatc
     1741 aacaatcggg gagccagcaa ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt
     1801 atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt tactgttaag
     1861 tttcccgact tactaacaac tcatcagagg ggggagaaat cctcccttac ccttgttcct
     1921 ttactttagg ttgaaaaaac aacagcgtca atagacctgc ctgctacgaa gcgagatctg
     1981 tgaaccgctt tccagttagc cttttttatc cagttggatc ttcttgatga tgttggtcgt
     2041 cgagcaaccg tcttcaaagt tgagcaccag cacttcgcca ccgttggccc agacttcttt
     2101 actcccggca atctcttctg gtttatagtc gccgcctttc accagcagat ctggcaagat
     2161 cccggcgatc aagcgctgcg gcgtgtcctc ttcaaacgac accacccagt cgaccgcttc
     2221 cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg aatccccttt
     2281 cagccgtttg gtggaggcat cgctgttcac agcgacaatc aaacggtcac ccagtttacg
     2341 ggcatttgcc agataagaga cgtgcccagc gtgcaggatg tcaaagacac cgttggtcat
     2401 caccactttt tcaccgcgtt tacgcgctgc cgctacagca agcttcagtt cctcttcggt
     2461 catcacacca aagccggtat ctgcacgtcc acgtactgcg ttttccagct cgatcggcga
     2521 aaccgtggat gtccccagtt tgccgaccac cacaccagcc gccgcattgg caaagaagca
     2581 ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc
     2641 gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca gcggcgcttt
     2701 acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag ccgagagttc
     2761 gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct tacatttacc
     2821 gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc ggtagcgctc
     2881 aaaatcagta ccttttggat caatcagcac cggaacaccc gctttacgcg ccagttggat
     2941 catctgctgt acgcttgcca gcgcaccttt ggcgtaatca gaaagcacca gcgcgccaat
     3001 cgaactcagt gcctgattaa tccgctcgtg cagcggctgc ggatcaaccc cttcgaaacc
     3061 ttcttcgaaa tccagacgga tcagctgttg gttgcgggaa agtacccgta atttggtgat
     3121 ggtcggatgc gtcggtacag aaacaaagtc gcatttgacg ttgacgtcgg ccagagattt
     3181 actgagcgcg cgcgccgcat cgtcaatgcc cgtcaacccg accaggcgtg cattagcacc
     3241 gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt cgatggtatt
     3301 cactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac cataccagta
     3361 acgatccagc atcacatcac caaccaccat cactcctgca cgttcaaact ctggcagcgt
     3421 tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac catacttcat
     3481 tcttccacca gccacttctg ccagcttgcc cgcaccagtt cacgctctgc ggtgaagcaa
     3541 tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc atcgcgcagc
     3601 gttgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc gttttgcgcc
     3661 agtagttcca gaatgcgcac gttgtctgac cagcgcgtta acttcggttt ttcatgagcg
     3721 tagcgcaaca ccagatattg agtaataaat tcgatatcgg taattccgcc ttcatcggct
     3781 ttgatatcaa agcgatcgcg atgtttattg ccgagatggg cacgcatttt ctcgcgcatt
     3841 tcccgcactt ctgtttgcag ggttttgcct tcgcgcggca gcgtcataat ctcccggcgt
     3901 actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc acgcaccagc
     3961 gcctgatgct cccacgtcca ggcctcgttt ttctgataat cagcaaatgc ttctgcggat
     4021 gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg catccacttc atacaaaatg
     4081 ccggaagagg ttcgcgtact gaacagatgc ataatgcgtt gcgccagacg cagataaaac
     4141 tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca atcatggagg
     4201 aagatcaggt caagatcgga actgtagcct aactcccagc cccccagctt gccgtagccg
     4261 accaccgcaa aaccacgccc ttcgcgttcg ttcaggtgat ttggcttacc gtagcgggca
     4321 accatttgaa cccacgcctg ctgaacgacg gcatcaatca tggcttctgc cagccaggtt
     4381 aagtgatcgc tcactttcat taccggtagc gtaccggcga tatccgctgc cgcaatgcgt
     4441 aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct gttgttcttc gtcatcttcc
     4501 ggcacgcgca gcaaatactg gcgcagttca tcacggtagg catccgtcgc cgttggttgg
     4561 tagagggtat ttggatcgag caattcatcc agcaataatg gataacgcgc tagctggctg
     4621 gcaatcatcg gcgatgcagc acacagagaa atcaaatgtt taagcgccgc agggaattca
     4681 ctaagcagct caagataagt ggtgcgagta acaatcccca ccagcaaggc ggtaatgcgc
     4741 gacagcgtga cagcagcgtc ttcacgcgca cagacatcac ttagcagatg tggcatcaga
     4801 tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct tatccagctc tttgcgaaaa
     4861 tcagcaatca gcattagcac ctgtttgcga tcatcctcgc taagatgcgc cagcactggc
     4921 gtggtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc cgacagcgac
     4981 tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg gcgcacattg
     5041 gccatatgtg cggtcagcac cccggtcaat tgcggccagt cagcaaagtc catcgcccac
     5101 gccagccgcg cacgggtaag ctcatcagaa ggaagcgtct gggtttgttc gtcgttaatg
     5161 ctttgcagca ggttttccag acgccgcagg aacagatacg ccactcgcaa ttgttcagca
     5221 tcgttttcag aaagcagatg cagcgcggca atggcgctga gcgttggcag taaagcgcgc
     5281 gattgcagcg acggttcgcg cccgccgcga atgagctgga acacctggac gataaattca
     5341 atttcgcgaa tgccgcctgc gccaagtttg atgttgtcgg tcaaaccgcg tcgacgcact
     5401 tcacgggcaa tcattccttt catgttgcgc agcgactgaa tcacgctgaa atcgatgtaa
     5461 cgacggaaaa caaacggacg cagcatcgca cgcaactcgt tagcatagac gccgtcgcta
     5521 tcccccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc ctgctcctgg
     5581 taataatctt ccagcgccgc aaaactcagc accagcgggc cactttcacc aaacggacgc
     5641 agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccag cactttaatc
     5701 agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg tccaccctgg
     5761 gtacaaccat gttccggcca ggcaaaaatc agatcgatat cagaggagaa attcagttcc
     5821 ccgccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc ctgcgcattg
     5881 cacggcgttc cccattcgcg gcagcaggcg tcatacagcc agtcacgcgc cgcaacaatc
     5941 agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac cagagccagc
     6001 gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag ctcgcgcatt
     6061 aacccggctt cgtcactcac attactgagc gcctcctgca accatgcagc gtaatgctgc
     6121 cattcgtcgg cctgcggcgg ttggctttcc agttccgtca gccactctgg atgcgcaatc
     6181 acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc gctaagtgat
     6241 tcctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg ctgtaacggt
     6301 gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc cgctgtgcaa
     6361 ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa tgcgttgccc
     6421 ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg cgacgacagg
     6481 atcgtagtaa cccgccagca acagtattga gtcaatatca cgcgtcaggc gtggcaattg
     6541 gtcacggtag cgatcgccta acggctggca gaaaacgctt ttaagttcag cagcatggcg
     6601 ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc attttactct gcgctttggc
     6661 atctaaaaac ggctgccagg ctttgcttac cagccattcg gtcaacgcca gcttcgccat
     6721 tgccgtttcg gtagagtaga ccgccgttac ggcagaaacc gcagaagcaa tggtcgcctc
     6781 gcattgagtc agcagatcac gtaagtgagt gctcgcttta cgcggcacga taccgccgaa
     6841 cagcatcagt gtatgacgga ccaggccaat ggctgccagc acctgctctt tcgccgcatc
     6901 gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca gctcgagcgc
     6961 cacttccagc ccctgttcca catcagcttt tgccgcaaca tgcaaaatgg tggtcggttt
     7021 gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgctgctt tgctcaggct
     7081 gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca ccgcacgcat
     7141 gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt cacccgcttt
     7201 cacttccccc tggtcgaggg cgatttcaat ttggctacca tcaacctcta ccagccactt
     7261 ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat cggcaggcaa
     7321 ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg gttcgctcaa
     7381 cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg caactttcat
     7441 ggtcatctca tagcgaccgt tttcgccacg aatacgcaag cccatatcgt gcccacgcag
     7501 ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat ggtgctcgcc
     7561 gcccagcgta ttgagatggt cacgcagcgc cgcaacggca ctgtgattaa caataaactt
     7621 taattcgatt tcctgagcca tagcctttac ttatcggtta tgtcacataa gtgacgatga
     7681 acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca aattgccatg
     7741 caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga cacagaggat
     7801 tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc gttccacttt
     7861 ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa ctttactcgc
     7921 acttagcgcc actgccgtct cacatgctga agaaacgcgc tatgtttctg atgaactgaa
     7981 tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg ttaacgccgg
     8041 cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg tgaaagacag
     8101 ctctggccgt accgcctgga tcccgttgaa acaacttagc acagagccaa gcctgcgctc
     8161 ccgtgtgcca gatctggaaa atcaggtcaa aacgctgacc gataaactca ccaatatcga
     8221 taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga gtgacagcgt
     8281 gatcaacggg ttaaaagaag aaaatcagaa actgaaaaac gagctgattg tcgcgcagaa
     8341 aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca tcatgcagtg
     8401 gtttatgtat ggcggtggcg tgctggggct tggcttgctg cttggtctgg tactgccgca
     8461 cctgatccca agccgcaaac gcaaagatcg ctggatgaac taaatcgcct tcttcgttgc
     8521 actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga agatttatct
     8581 ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca gagattgggt
     8641 ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg taggccgcga
     8701 ttttcctgtt tttctgcatc cgcaaacgca tgaagagtat gcgctggcac gtactgaacg
     8761 gaaatccggt tccggttaca ccggttttac ctgctatgcc gcaccggatg tcacgctgga
     8821 agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg ataacggcga
     8881 gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc gccatgtttc
     8941 ccccgctttt ggcgaagatc cgttacgcgt gttgcgcgta gcgcgttttg ctgcgcgtta
     9001 tgcccacctc agtttccgta ttgccgatga aactctggcg ctgatgcgcg agatgaccca
     9061 tgcaggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg aaaacgccct
     9121 caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggcg cactgcgcgt
     9181 tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt ggcatccgga
     9241 aatcgacacg ggtattcata ccttaatgac gctctcaatg gcggcgatgc tgagtccgca
     9301 ggtcgatgtc cgtttcgcaa cgttatgcca cgatctcggt aaaggactga cgccgccaga
     9361 actttggcca cgtcatcatg gtcatggccc ggcgggtgtg aagttagtgg aacaactatg
     9421 ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtag ctgagtttca
     9481 cgatctcatc cacacctttc caatgctgaa cccgaaaacc attgttaaat tgtttgattc
     9541 cattgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca gcgaggctga
     9601 cgtgcgcggc agaaccggtt ttgaatcggc ggactacccg caaggccgct ggttgcgcga
     9661 agcctgggaa gtggcgcagt cagtgccgac aaaagctgtc gttgaagcag gatttaaagg
     9721 tgtggagatt cgtgaggagc tgacccgacg gcggattgcg gcggtagcca gctggaagga
     9781 acagcgttgc ccaaaacctg agtgagtgtt ggatattttg ccggatgcga cgcaagcgtc
     9841 ttatccggct acatgtcaac gacagttgta ggcctgataa gacgcgcgag cgtcgcatca
     9901 ggcgttgatt gccggatgcg gcgtgaacgc cttatccggc ctacgcaatc tgataagacg
     9961 cgtcagcatc atatccgaca ctgttcttca catcaaaaga acacaacgta caccgcagcc
    10021 gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg
    10081 aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc
    10141 gggatatcgc cggtagtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc
    10201 atcatcatcg gcaccgccag caggaacgag aactcggaag cagcgtaacg gctcaccccc
    10261 atcagcatcc cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga
    10321 cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca
    10381 cgcggctctt tcggcttcag gcattcggcg gcaatcagca acagaccgcc aacgaccagt
    10441 gcatacatga catttatcgg gttaaacaat gatttaatcg tgtcgtggaa caacagcccc
    10501 agtaccaccg ccggaatcat ccccagcaag atgtggatca gcgttaaacg acctttgctt
    10561 tctccttcgt gctgcaacgg gcgaccaaag tggatgccaa tcaggccaaa cagacgccgc
    10621 cagaacatca ctactaccgc cagaattgat cctaactgga tcacgacttc aaaggttttc
    10681 gccgtgtcgc cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat
    10741 accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag
    10801 tgcatatcgc tcatcaataa acccctaaat tataaaaatg taacgcttgt ccgaactact
    10861 gcagtatgac caggttataa tcgtttggtt taacagctgt aaaattaatt attttctttc
    10921 agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc
    10981 ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc
    11041 tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt
    11101 tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg
    11161 ttatcccacg ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg
    11221 taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc
    11281 tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat
    11341 tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat
    11401 gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg
    11461 tgggttgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg
    11521 tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt
    11581 gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat
    11641 cgccgcctgt cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt
    11701 tgctaccgct tttggtgcca tcgcaccaat tggctgggat ctcactggag taatggcggg
    11761 aacctggtta ctgaccgtgc tattgagtgg ttactcgtcg ctgggagcga ttgtcagtgc
    11821 actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat
    11881 gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca
    11941 ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct
    12001 ggtggatcta catgacctga tagccttcgt cgggcttgcc cagccgttgc tggcaccatg
    12061 ccgccagaaa ttccacacag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa
    12121 cagcccagat attggcgctt tgtgcatact ccggcaatac tcgcaccagt ttgccactct
    12181 ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc tccagcgccc
    12241 attgcagcac aatctcgccg ctattggagg aaagatgtcc gcttaccttc accgattttt
    12301 tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca
    12361 ggcagtcatg acgacttaat tcctgtaagg attgtggctg cggatatttt tgcagatact
    12421 caggtgctgc gcataatatt cttttatttt ttgtcaaaag atgcgcaata taataatcag
    12481 gaattgcgtc attaatacga atatccagat caatattatc ctgcactaaa tcaatttgtc
    12541 gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcatcagt tcggtaatag
    12601 ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatgcggatc atcccctccg
    12661 ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg aggatttcca
    12721 gcgcatgttc gtaacaacgc tggccacttt cagtcagcgc cacgccgcga gcggagcggt
    12781 tcagcagagt tgttgccagc gtattttcaa ggatttggat ccgcttggtg acaaatgcag
    12841 gcgtttgccc aagcgtcgcc gctgcagcac taaaactccc gctatggacg atttccacca
    12901 gtacctgcaa gtctttggct aaaggccagc tatttagcat tgttaaatat ccgcagtttt
    12961 gataaacgca gtgtatttcg caaaacatta ccattccctg ctctggcgca atattcgttc
    13021 ttattttcgc gttgattcac gaactgtgaa ttacatattc acggtggcaa aaaatataaa
    13081 accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac ataaactgga
    13141 gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac ggagattgtc
    13201 gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga taaactaaaa
    13261 cagctaaagg atgccgaaac atcgtcgatg ggaaaaatca tctaccacac gatgttcgat
    13321 aacatgcaaa aagcgatcga cctgaatcgt cctgcctgtc aggacaccgg cgaaatcatg
    13381 ttttttgtta aggtcggttc ccgtttccca ctgcttggcg agctgcaaag catactcaaa
    13441 caagccgtgg aagaggcaac cgtcaaagcg ccactgcgtc acaatgcggt agaaattttt
    13501 gacgaagtaa acaccggcaa aaataccggc agtggcgtac cgtgggtcac ctgggatatc
    13561 gtccccgacg gtgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta
    13621 cctggccgat cgaaagtgtt aatgccgtca gaaggctacg aaggcgtagt gaaattcgtc
    13681 ttcgaaaata tctccaccct cgccgtaaac gcctgtccgc cggtacttgt gggcgttggc
    13741 atcgctacct cggtggaaac cgccgcagtg ctctcgcgta aagccatttt gcgcccgatt
    13801 ggcagccgcc atcccaatcc aaaagcggca gagctggagc tacgcctgga agaaggactc
    13861 aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat gggcgtgcat
    13921 atcgaatctg ccgctcgcca tccgtcaacc atcggcgttg ctgtttctac cggctgctgg
    13981 gcgcatcgtc gcggcacact gcgagttcat gccgatctca cctttgaaaa tctgtctcat
    14041 acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag
    14101 atattcgcgt cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgtgacgttt
    14161 gtcaccgccg attgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga
    14221 ttttccacgc tggccccatc gtgcgcaaaa acggcgacaa atgggagatg gtgtccgtcg
    14281 gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag cagaccggcg
    14341 tgaaactggt ggtcggcaaa ggcggaatgg ggccgctgac cgaagaaggc tgccagaaat
    14401 tcaaggcgct gcatgtgatt ttcccggctg gctgtgcggt actggcggca actcaagtgg
    14461 aagagattga agaggtacac tggacagaac tcgggatgcc agagtcgctg tgggtctgcc
    14521 gggtcaaaga gtttggcccg ctgattgtct ctatagatac ccacggcaac aacctgatag
    14581 ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtagaagag atctgcgagc
    14641 acgtccacta catcaaataa ccctcccgga gaggctcgcc cctctccttt ttctcaggca
    14701 taacacgatg aaaccttcca ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc
    14761 cattattgct ctacttccgc ttcccgcagg gctggagagt catacctggc tctactttgc
    14821 cgtttttact ggcgtgatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc
    14881 gatggtgggt atatccatca tcgccattct ttcgccgtgg ctgctgttta gcccggagca
    14941 gctcgcccag ccaggtttta agttcaccgc caaatccctc tcctgggcgg tttctggttt
    15001 ttctaattca gttatctggc ttattttcgc cgcctttatg tttggtacgg gctatgaaaa
    15061 aaccgggctt ggacgtcgca tcgctctgat tctggtgaaa aaaatggggc atcgcacgct
    15121 gtttctcggc tatgcagtga tgttctccga gctgatactc gcgcctgtaa caccgtccaa
    15181 ctcggcgcgt ggtgcgggga ttatctatcc catcatccgt aacctgccac cgctctatca
    15241 atcacaacca aacgacagca gttcgcgcag cattggctcg tacatcatgt ggatggggat
    15301 tgttgccgat tgcgtgacca gcgccatttt cctgacggcg atggctccaa atttgctgtt
    15361 aattggactg atgaaaagcg catctaacgc cacgctgagc tggggcgact ggttcctcgg
    15421 gatgttgccg ctcagtattt tactggttct gctggttccc tggctggctt acgtgctgta
    15481 cccgccgata ctgaagtctg gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc
    15541 aatgggcccg ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcactggt
    15601 gctgtggatt tttggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc
    15661 gctgatgctg cttctgcgca ttatctgctg ggacgacatt gtcagtaata aagcggcgtg
    15721 gaacgttttc ttctggctgg cctcgcttat caccctcgct accggactca acaacaccgg
    15781 ttttattagc tggtttggca aactgttagc aggcagctta agcggttatt cgccaacgat
    15841 agtgatggtg gcgttgattg tggtctttta tctgctgcgc tacttttttg ccagcgccac
    15901 agcgtatacc tccgctctcg caccgatgat gattgccgcc gcgctggcga tgccggaaat
    15961 cccgctgccg gtgttctgcc tgatggttgg tgcggcaatt ggtctgggga gcattcttac
    16021 accatacgcc accggaccca gtccgattta ctacggtagt ggttatctgc caacggtgga
    16081 ttactggcga ctgggggcaa tttttgggct gatattcctc gtgttgctgg tgattaccgg
    16141 cttactgtgg atgcccatgg tgttgcttta agtgaggacg gtgccggaaa ttgctctccg
    16201 gcacctaata gttttacgcg gccggtaact ctgccagcgg ccagcgcgga cgcacgctaa
    16261 cgccaagatc cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg
    16321 cgccgttatc agtacaaaat tccggacgcg cgtagaagac ttcgccacgg cgttttttca
    16381 tcatttcagc cagcttcgcc cgtaacgtgc ggttagcact cacgccacct gccattacca
    16441 gtcgcttaaa gcccgtctga tccagcgctc gcttacactt aatcatcaac gtatcgacca
    16501 ccgcatcttc aaaggcgcgg gcgatatcag cacgcgtctg gtcgtcggtg ccgttatcac
    16561 gaatggtatt tgccgcgaag gttttcagac cagaaaagct gaaatccagc cccggacggt
    16621 cggtcatcgg gcgcgggaag acaaagcgcc cggcagtacc ctgagccgcc attttcgaca
    16681 gtaatggccc gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac
    16741 cagccgcatc atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga
    16801 ttaactgcgt atgaccgccg gaaaccagca gcgcgacaaa cggaaattcc ggtgggttat
    16861 cttccagcat cggcgctaac agatgccctt ccatatggtg taccgggatc gccggaacgt
    16921 cccaggcaaa cgccagagaa cgccccacgg tcgcgccaat cagcagcgcg ccgactaatc
    16981 cagggcctgc ggtataggcc acagcatcaa tgtcttttgc cgttaaacct gactccttta
    17041 gcgccgcctg gatcaacggt acggttttac gcacgtgatc gcgggaggcc agttcaggca
    17101 cgacgccgcc gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac
    17161 ctttttcatc gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta
    17221 cacgcatgac ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga
    17281 tgtaaaactt tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt
    17341 ccctttcaaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca
    17401 ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag
    17461 gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt
    17521 caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga
    17581 aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa
    17641 actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttcccgagag cgttgctctc
    17701 cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt
    17761 cgtttatgaa ttgctaaaaa tcggggccta tggctggacg aatcccacgc gtattcatta
    17821 atgatctgct ggcacgcact gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa
    17881 agcagggcaa gaatttccac gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca
    17941 ccgttaacgg tgagaaacag ttttaccact gctttggatg tggcgcgcat ggcaacgcga
    18001 tcgacttcct gatgaactac gacaagctcg agttcgtcga aacggtcgaa gagctggcag
    18061 caatgcacaa tcttgaagtg ccatttgaag caggcagcgg ccccagccag atcgaacgcc
    18121 atcagcggca aacgctttat cagttgatgg acggtctgaa tacgttttac caacaatctt
    18181 tacaacaacc tgttgccacg tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg
    18241 aggttatcgc ccgctttgcg attggttttg cgccccccgg ctgggacaac gttctgaagc
    18301 ggtttggcgg caatccagaa aatcgccagt cattgattga tgcgggcatg ttggtcacta
    18361 acgatcaggg gcgcagttac gatcgtttcc gcgagcgggt gatgttcccc attcgcgata
    18421 aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg caacgatact cccaaatacc
    18481 tgaactcgcc ggaaacggac attttccata aaggccgcca gctttacggt ctttatgaag
    18541 cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg
    18601 tggcgctggc gcaatacggc attaattacg ccgttgcgtc gttaggtacg tcaaccaccg
    18661 ccgatcacat acaactgttg ttccgcgcga ccaacaatgt catttgctgt tatgacggcg
    18721 accgtgcagg ccgcgatgcc gcctggcgag cgctggaaac ggcgctgcct tacatgacag
    18781 acggtcgtca gctacgcttt atgtttttgc ctgatggcga agatcctgac acgctggtac
    18841 gaaaagaagg taaagaagcg tttgaagcgc ggatggagca ggcgatgcca ctctccgcat
    18901 ttctgtttaa cagtctgatg ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt
    18961 tgagtacgct ggcactgcca ctgatatcgc aagtgccggg cgaaacgctg cgaatatatc
    19021 tacgtcagga attaggcaac aaattaggca tacttgatga cagccagctt gaacgattaa
    19081 tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca
    19141 tgcgtatact tatagggttg ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc
    19201 ttgagaatct ggatgaaaat aagctccctg ggcttggctt attcagagaa ctggtcaaca
    19261 cttgtctctc ccagccaggt ctgaccaccg ggcaactttt agagcactat cgtggtacaa
    19321 ataatgctgc cacccttgaa aaactgtcga tgtgggacga tatagcagat aggaatattg
    19381 ctgagcaaac cttcaccgac tcactcaacc atatgtttga ttcgctgctt gaactgcgcc
    19441 aggaagagtt aatcgctcgt gagcgcacgc atggtttaag caacgaagaa cgcctggagc
    19501 tctggacatt aaaccaggag ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc
    19561 gatcgggaag cccccgacag ccgcactgag aggcagcggc aaatatataa gtacgccctc
    19621 gtaattatcg ttggcggtaa acaaccgttg gatttcagcg ttaacagctg aaggacatcg
    19681 ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga taccgtctta tggagcaaaa
    19741 cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag gagcaaggct atctgaccta
    19801 tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat tcagatcaga tcgaagacat
    19861 catccaaatg atcaacgaca tgggcattca ggtgatggaa gaagcaccgg atgccgatga
    19921 tctgatgctg gctgaaaaca ccgcggacga agatgctgcc gaagccgccg cgcaggtgct
    19981 ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg gtacgcatgt acatgcgtga
    20041 aatgggcacc gttgaactgt tgacccgcga aggcgaaatt gacatcgcta agcgtattga
    20101 agacgggatc aaccaggttc aatgctccgt tgctgaatat ccggaagcga tcacctatct
    20161 gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg
    20221 ctttgttgac ccgaacgcag aagaagattt ggcacctacc gccactcacg tcggttctga
    20281 gctttcccag gaagatctgg acgatgacga agatgaagac gaagaagatg gtgacgacga
    20341 taccgccgat gatgacaaca gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact
    20401 gcgcgctcag tacattgtaa cgcgtgacac catcaaagcg aaaggccgca gtcacgctgc
    20461 cgctcaggaa gagatcctga aactgtctga agtattcaaa cagttccgcc tggtgccgaa
    20521 gcagtttgac tacctggtca acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga
    20581 acgtctgatc atgaagctct gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac
    20641 cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac gcggcaattg cgatgaacaa
    20701 gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg catcgcgccc tgcagaaact
    20761 gcagcagatt gaagaagaaa ccggcctgac catcgagcag gttaaagata tcaaccgtcg
    20821 tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa gagatggttg aagcgaactt
    20881 acgtctggtt atttctatcg ccaagaaata caccaaccgt ggcttgcagt tccttgacct
    20941 gattcaggaa ggtaacatcg gcctgatgaa agcggttgat aaattcgaat accgccgtgg
    21001 ttacaagttc tccacctacg ctacctggtg gatccgtcag gcgatcaccc gctctatcgc
    21061 ggatcaggcg cgcaccatcc gtattccggt gcatatgatt gagactatca acaagctcaa
    21121 ccgtatttct cgccagatgc tgcaagagat gggccgtgaa ccaacgccgg aagaactggc
    21181 tgaacgcatg ctgatgccgg aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc
    21241 aatctccatg gaaacgccga tcggtgatga tgaagattcg catctggggg atttcatcga
    21301 ggataccacc ctcgagctgc cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac
    21361 gcacgacgtg ctggctggcc tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg
    21421 tatcgatatg aacaccgacc acacgctgga agaagtgggt aaacagttcg acgttacccg
    21481 cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc
    21541 tgaagtgctg cgtagcttcc tggacgatta atcggtaggc cggatcaggt gttacgccgc
    21601 acccggcact agtccctccg cacaaacgcc accttttcgg tggcgttttt atcgcccacg
    21661 caccaccagc gcctggtcca gctcgcgata cgcttcaacc agtttctcca gtgaaacgcg
    21721 acttaaaccg ctgggatttg gcaggaccca aatctgcgtc gaaccaatgg tgagagtttg
    21781 tttcccccac tgtgcaccgc gctggctgaa tccctgttca tatgcttgtt tgcccagaat
    21841 cgccaacgcc tgcggctgat aatcttctat tttttcaatt agcttacgcc cgcctgcgtg
    21901 cagctcctgc tttgacactt cattggcttg caccgttgga cggtctacca gtttggtgac
    21961 gccacaacga taatccagca gatgctgtgc ctcctgcggc ttcaactgac ggtcggtaaa
    22021 cccggcctga tatatcacct tccagaagcg gtttgccgga tgagcaaagg gaaaacccgt
    22081 tccggcggat gaaagaccag ggttgatacc gcaaaacacg acccgtaacc ctggagccaa
    22141 aatatcctca accatattta ctcctttcta acatcaccag gaaaagtata acggattgaa
    22201 aatacattgt ttataaaaac agcaggcgcg cggtaatggc tggattgcga cacggagtta
    22261 ctttataatc cgctaccatg gccccttagc tcagtggtta gagcaggcga ctcataatcg
    22321 cttggtcgct ggttcaagtc cagcaggggc caccagacat agcaaaggct gacgagaaat
    22381 cgtcagcctt tttcttttta tatatcagtt actttgcgtg ccagtaagcc gccgcgcgta
    22441 cccgctgtgg gtcatactgt tccgcttcaa agcgacggct taaattctta acgactttgc
    22501 cttcgccggt tatccagatg aagtaatcat cggcagggat ttgcatctgc gccagacgcg
    22561 catctaccgc ctgctcatca tgtgccagcc attcgatatt aaaaccatca aggtgcgcga
    22621 gataatcctg acaggcgtta tcccgcacgc taaccagcgc gctaacttgc ggtttaacgg
    22681 caagtttgct caacgtttcc aggcggcggc gcagtgcggg cattccggat tcatcgcaga
    22741 catacagctg atacgcgtaa tcctctggca ccaccagcga accgcgcgga cctgccaccg
    22801 tgagtttatc gcccgattgc gcctgcattg cccagctgct ggcgacgcca ccttcgtgaa
    22861 taaagaaatc aatcgccagt tcatggtgta gttcgtcata cagcggcgta tagtcacgcg
    22921 acggtgggcg tggtccttcc ggccagacga tgccctcttc cgttaccgtt ggcggcacaa
    22981 agtgagcgtc aggttgagga aagaagagtt tgctgtgatc gtcaaagccg cgcgatgtaa
    23041 aaccgtccag cgcctcgccg ccgaggacaa tgcgctgaaa accggcgctg atgcgctcaa
    23101 cgcgtaacac agtcagttca cggaagcgca gatcattgcg aacgcgctgt gggtagcggg
    23161 gggtgttatt cattgttatc gccttcgtga tggtaatcag atatatctaa ataaattcca
    23221 caaatgataa tgattgttaa tcatgataaa tgcaagtgat ttgtagcact gatatatctg
    23281 tagtctgata agacgcgcaa cctcttatca agcatcattg ctcaatgccg gatgcggcgt
    23341 gaacgcctta tccggcctac atgttgagta aacctgcgtt taaaaatcat ttttgtactt
    23401 gcagtaatgc ttatatcaga tataaattag atatatctaa ttaagcaaag ggaggctgat
    23461 atgagccatc atcacgaagg gtgctgtaaa catgaaggcc agccacgcca tgagggctgc
    23521 tgcaaaggtg agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt
    23581 caatgctgcg gtggtcgcca cggtcgcggc ggcggtcgtc gacaacgttt ctttggtcac
    23641 ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgag
    23701 ttaattaaag cgattgaaaa tctgacccag gggaattaca ccccaagccc gggcgtaatc
    23761 tacccaacgc tggattttct gcaggagcag tcgctgatta ccattcgcga agaggaaggt
    23821 ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa
    23881 caggtagaga tgattgaaga acgcatcaaa gcgcgttgcg ttggcgctgc gctgcgccag
    23941 aacccgcaaa tgaagcgggc gctggataat tttaaagcgg tgctggattt acgcgtcaac
    24001 cagagcgata tcactgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct
    24061 tttgatatta cccaactgga ttaatcaccg attgccggat gcagcgtacc ctacacaccc
    24121 cgtaggcctg ataagatgcg ccagcatcgc atcaggcatt gtgctccaac cgctggatcc
    24181 ggcataccga ttaatgcagc accgtcaccg cgtcttccaa tcggctggcg cggtgtttca
    24241 ccatcgccga cacctgcgca ctctcttcca ccaactcggc gtttttctgg gtgatcaggt
    24301 taagctcatc cactgcacgg gtcaggctgg aaagcccatc ggcctgttcc agcgttgaat
    24361 ggctaatctg agctatcaac tgggtgacgt tttttacctg cgccacaata tcttccatcg
    24421 tccgtccggc ggcgtgtacc tgctgcgaac cagattgcac cttatcagca ctggcatcaa
    24481 tcagcttgcg aatatcgttg gcagcattgg cgctgcggct ggctaaatga cgcacttccc
    24541 ccgccaccac tgcaaaacct ttgccctgtt cgccggcacg cgccgcttcc accgccgcat
    24601 tcagggccag aatattggtc tgaaacgcaa tatcgttaat cagcgaagta atggtgccaa
    24661 tgcgctgggt actgtcggca atatcgtcca ttgtcttgat caccgtagtc atcgcctccc
    24721 cgccctgcac tgccgcatta ctggcggtga tagaaagttt atcggcagcc gacgccgtgg
    24781 cagagttctg tttcaccgac gccgccattt ggttcatggt cgccaccgtt tgctgaacgt
    24841 tatcaactgt ctgctgggta tgttcgttca gttcatcggt gcctttcgcc agcgtctcac
    24901 tgccgtttct gacactggac acctggcttg agacatcatt aattagccaa cggcacatca
    24961 ggccaagctg ccctaccgca cgtaatgtca gccccagctc atcgctgcgc ttcagatgct
    25021 caacactatt acgctctccg gtcgccacct tcagtgcctg acaggcgaca ttttctatcg
    25081 ggcggacaat ctgccattca aaacaagcgc ttgccaacaa taccactaat gcacagagga
    25141 tatacgtcac caccggggca gcaacaaacc agagcatggc cgccagcaag ataaacatca
    25201 gggtcatcac tccacgcgcc cgccagcgaa gcggtaatga aggcagtttt cccaaccaac
    25261 ctttacgcac caccaggcct ttatgaatac gcttactggt ccgtccggcg ttcaacgctt
    25321 tgtacagcgg ctccaccgcc gcgatctctt catccgtcgc ccgggtacga atcgacatat
    25381 agccacggat ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc caataatggt
    25441 cgccattttt gcggcgattt ttcacgatgc cgctccaggg ctcccctttt ttcagggtga
    25501 accacatatc cgcaaacgcc gcttttggca tatccgggtg acgcaccatg ttgtgcggtt
    25561 gcccctgtaa ctcctgcaag gtatagccgc tcacctgcac aaaagtgtca ttagcatgag
    25621 tgatatagct ttgcagatcg gtagtggaca tcagagtggt gtcgtccgcc agcggggtat
    25681 tttgctgggt gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt tatatggttg
    25741 ttaacttctt gtcagagttt atgtcggccc cgctgcggtt atctttaacc aattaatttg
    25801 atttagatcg caatttgcga ctaaagcaca aatctaattc cttgatttaa aatactttca
    25861 ctctgttact atacgaaaac gttaattacc ttgcccaaaa atcaggcaat tattgccctg
    25921 aaagcgtgca tttgcgcagc aatcatcaaa tccatacccg acaaaaaccg tgcaaaataa
    25981 cagcaaatgt tagcaaatag cattaaatat tgcgcaaatg ataaccacat ttgtgtttat
    26041 cccgattttc gcgatcgcag ccggagtggc gcaatccctg caatacttaa atcggtatca
    26101 tgtgatacgc gagcctccgg agcatatttt gaacaggtta ccttcgagcg catcagcttt
    26161 agcgtgcagc gcccacgccc tgaatctcat tgaaaagcga acgttggatc atgaggagat
    26221 gaaagcactt aaccgggagg tgattgaata cttcaaagag catgtcaatc cggggttttt
    26281 agagtatcgc aaatctgtta ccgccggcgg ggattacgga gccgtagagt ggcaagcggg
    26341 aggtttaaat acgcttgtcg acacccaggg acaggagttt atcgactgcc tgggaggttt
    26401 tggaattttc aacgtggggc accgtaatcc agttgtggtt tccgccgtac agaatcaact
    26461 tgcgaaacaa ccgcttcaca gccaggagct gcttgatccg ttacgggcga tgctggcgaa
    26521 aacccttgcg gcgctaacgc ccggtaaact gaaatacagc ttcttctgta atagcggcac
    26581 cgaatccgtt gaagcggcgc tgaagctggc gaaggcttat cagtcaccgc gcggcaagtt
    26641 tacttttatt gccaccagcg gcgcgttcca cggtaaatca cttggcgcgc tgtcggccac
    26701 ggcgaaatcg accttccgca aaccttttat gccactgctg ccagggtttc gtcatgtacc
    26761 gtttggcaat atcgaagcca tgcgcacggc tcttaacgag tgcaaaaaaa ccggtgatga
    26821 tgttgctgcg gtgatccttg agccgattca gggcgaaggt ggcgtaattc tgccgccgcc
    26881 aggctatctc accgccgtgc gtaagctgtg cgatgagttc ggcgcactga tgatcctcga
    26941 tgaagtacaa acgggcatgg ggcgcacggg caagatgttc gcctgcgagc atgagaacgt
    27001 acagccggat atcctctgcc ttgccaaagc gctcggcggt ggcgtgatgc cgattggcgc
    27061 aactatcgcc actgaagagg tgttctcagt cctgttcgac aatccgttcc tgcataccac
    27121 cacctttggc ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca atgtgttgct
    27181 ggagcagaac ttaccggctc aggctgaaca aaaaggcgat atgttgctgg acggtttccg
    27241 tcaactggcg cgggaatatc ccgatctggt acaggaagcg cgtggcaaag ggatgttgat
    27301 ggcgattgag tttgttgata acgaaatcgg ctataacttt gccagcgaga tgttccgcca
    27361 gcgcgtactg gtggccggaa cactcaacaa cgctaaaacg atccgcattg aaccgccact
    27421 gacactgacc attgaacagt gtgaactggt gatcaaagcg gcgcgtaagg cgctggcggc
    27481 catgcgagtc agtgtcgaag aagcgtaata tcaaatcgga tggcgattcg acgtcgccat
    27541 ccgatctttt tatacgatac gtacgcccgc aggcatcatc cgctccggcg ttaacagcac
    27601 gctttcactg ccgtcatcgg tttccgcaca caacagcatg cattccgacg tttcaccgcg
    27661 cattttcgct ttttgcagat tgcataagac caccactgtt tttcccatca gctcttcttc
    27721 gctgtagtac ggcaccagac tggtcacggt ttgtagcgtt ttttccccca catcaacctg
    27781 tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct ttccgacgcg
    27841 catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgcttcct cctttcgagt
    27901 aaaattttac taaactatag caaaaaattt tctcaatcct gtaggctaaa aatggagaat
    27961 gcaggcgtga tcacattcgc aagccgctgt gttaccgtta cagcgtcaaa gaaacgcgct
    28021 ttatttactg aaaacaggtg acccgataag cacttcctct acaatggggg cgcacatcag
    28081 ggaaagtaaa aaaggtaaac atggcaacac taaaagacat cgcaatcgaa gctggcgtat
    28141 ccctggcgac agtatccagg gtcttaaatg acgatccgac attgaatgtg aaagaagaga
    28201 cgaaacatcg cattctcgag atcgccgaaa agctggagta caagaccagt agcgcccgta
    28261 aactccagac aggcgcagtt aaccaacacc atattctggc tatctacagc taccagcagg
    28321 agctggagat caacgatcct tactatctgg cgatccgcca cggcattgaa actcagtgcg
    28381 aaaagctggc gatcgaactg accaactgtt atgaacacaa cggcttacca gacattaaaa
    28441 acgtcaccgg tattttaatt gtcggcaaac ccacgcccgc cctgcgcgcc gctgccagcg
    28501 cattgaccga caatatctgt tttatcgact ttcacgaacc cggcagcggt tacgatgcgg
    28561 tggatatcga tctggcacgt atcagtaaag aaatcatcga tttctatatc aatcagggcg
    28621 ttaatcgtat tggttttatt ggcggtgaag acttacccgg caaggcggat attcgtgagg
    28681 tcgcctttgc ggaatatggt cgtctgaaac acgtggtacg cgaagaggat atctggcgcg
    28741 gcggtttttc cagttcgtcg ggctatgaac tggcaaaaca gatgctggcg caggaagact
    28801 atccgaaggc actgtttgtt gcttccgatt ccattgctat cggcgtactg cgggcaattc
    28861 atgaacgtgg cctgaacatc ccacaggata tttcgcttat cagcgttaac gatatcccca
    28921 ccgcgcgatt tacctttccg ccgctctcca ccgtgcgcat ccattccgag atgatgggaa
    28981 gtcagggcgt taacctggtg tatgaaaaag cccgcgatgg tcgcgcgctg ccgctgttgg
    29041 tcttcgttcc cagcaaatta aaactgcgcg gcacgacccg ttaaatcccc ttacacactg
    29101 tccggcaatc gtttttgccg gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa
    29161 atgcatttac ctgctatttt ttagtaaaaa ttttactaaa ctccccagca attacacaaa
    29221 ctaccatcac catgaatggt tccgatttct ctctaccggg aggccctatg aatcgctggg
    29281 aaaacattca gctcacccac gaaaaccgac ttgcgccgcg tgcgtacttt ttttcatatg
    29341 attctgttgc gcaggcgcgt acctttgccc gcgaaacgag cagcctgttt ctgcccttaa
    29401 gcggtcagtg gaatttccac ttttttgacc atccgctgca agtgccagaa gccttcacct
    29461 ctgagttaat ggctgactgg gggcatatta ccgtccccgc catgtggcaa atggaaggtc
    29521 acggcaaact gcaatatacc gacgaaggtt ttccgttccc catcgatgtg ccgtttgtcc
    29581 ccagcgataa cccaaccggt gcctatcaac gtattttcac cctcagcgac ggctggcagg
    29641 gtaaacagac gctgattaaa tttgacggcg tcgaaaccta ttttgaagtc tacgttaacg
    29701 gtcagtatgt ggggttcagc aagggcagtc gcctgaccgc agagtttgac atcagcgcga
    29761 tggttaaaac cggcgacaac ctgttgtgtg tgcgcgtgat gcaatgggcg gactctacct
    29821 acgtggaaga ccaggatatg tggtggtcgg cggggatttt ccgcgatgtt tatctgatcg
    29881 gaaaacaact cacgcatatt aacgatttca ccgttcgtac cgactttgac gaagcctatt
    29941 gcgatgccac actttcctgc gaagtggtgc tggaaaatct cgccgcctcc cctgtcgtca
    30001 cgacgctgga atataccctg tttgatggcg aacacgtggt gcacagcagc gccattgatc
    30061 atttggcaat tgaaaaactg accagcgcca gttttgcttt tactgtcgaa cagccgcagc
    30121 aatggtcagc agaatcccct tatctttacc atctggtcat gacgctgaaa gacgccgacg
    30181 gcaacgttct ggaagtggtg ccacaacgcg ttggcttccg tgatatcaaa gtgcgcgacg
    30241 gtctgttctg gatcaataac cgttatgtga tgctgcacgg cgtcaaccgc cacgacaacg
    30301 atcatcgcaa aggccgcgcc gttgggatgg atcgcgtcga gaaagatctc cagttgatga
    30361 agcagcacaa catcaactcc gtgcgtaccg ctcactaccc gaacgatcca cgtttttacg
    30421 aactgtgtga tatctatggc ctgtttgtga tggcggaaac cgacgtcgaa tcgcacggct
    30481 ttgctaacgt cggcgatatc agccgcatta ccgacgatcc gcagtgggaa aaagtttacg
    30541 tcgagcgcat tgttcgccat attcacgcgc agaaaaacca tccgtcgatc atcatctggt
    30601 cgctgggcaa tgaatccggc tatggctgta acatccgcgc gatgtaccat gcggcgaagg
    30661 cgctggatga cacgcgactg gtgcattacg aagaagatcg cgatgctgaa gtggtcgata
    30721 ttatttccac catgtacacc cgcgtgcctc tgatgaatga gtttggtgaa tacccgcatc
    30781 cgaagccgcg catcatctgt gaatatgctc atgcgatggg gaacggaccg ggcgggctga
    30841 cggagtacca gaacgtcttc tataagcacg attgcattca gggacattat gtctgggaat
    30901 ggtgcgacca cgggatccag gcgcaggatg acaacggcaa cgtctggtac aaattcggcg
    30961 gcgactacgg cgactatccc aacaactata acttctgtct tgatggtttg atctattccg
    31021 atcagacgcc gggaccaggc ctgaaagagt acaaacaggt tattgcgccg gtaaaaatcc
    31081 acgcgctgga tctgactcgc ggcgagttga aagtcgaaaa taaactatgg tttaccacgc
    31141 ttgatgacta caccctgcac gcagaggtgc gcgccgaagg tgaaacgctc gcgacgcaac
    31201 agattaaact gcgcgacgtt gcgccgaaca gcgaagcccc cttacagatc acgctgccgc
    31261 agctggacgc ccgcgaagcg ttcctcaaca ttacggtgac caaagattcc cgcacccgct
    31321 acagcgaagc cgggcattct atcgccactt atcagttccc gctgaaggaa aacaccgcgc
    31381 agcctgtacc tttcgcaccg aataacgccc gtccgctgac gctggaagac gatcgtttga
    31441 gctgtactgt tcgcggctac aactttgcga tcaccttctc aaaaacgagt ggtaaaccga
    31501 catcctggca ggtgaatggc gagtcgctgc tgacccgcga gccaaagatc aacttcttca
    31561 agccgatgat cgacaaccat aagcaggagt acgaaggact gtggcagcca aatcatttgc
    31621 agatcatgca ggagcatctg cgcgattttg tcgttgagca aagtgatgat gaagtgttgc
    31681 tagtcagccg cacggttatt gccccaccgg tgtttgactt cgggatgcgc tgcacctaca
    31741 tctggcgcat cgctgccgat ggccaggtta acgtggcgct ttccggcgaa cgttatggcg
    31801 actatccgca catcattccg tgcatcggtt tcaccatggg aattaacggc gaatacgatc
    31861 aggtagcata ttacggacgt ggaccgggcg aaaactacgc cgacagccag caggctaaca
    31921 tcattgatat ctggcgcagt accgtcgatg ccatgttcga gaactatccc ttcccgcaga
    31981 acaacggcaa ccgtcagcat gtccgctgga cggcactgac taatcgccac ggcaacggtc
    32041 tgctggtggt tccgcagcgc cccattaact tcagcgcctg gcgctatacc caggaaaaca
    32101 tccacgctgc ccagcactgt aacgagcttc agcgcagtga tgacatcact ctgaatctcg
    32161 accaccagct gcttggcctc ggctccaact cctggggcag cgaggtgctg gactcctggc
    32221 gcgtctggtt ccgtgacttc agctacggct ttacgttgct gccggtttct ggcggagaag
    32281 ctaccgcgca aagcctggcg tcgtatgagt tcggcgcagg gttcttttcc acgaatttgc
    32341 acagcgagaa taagcaatga ggatcatcga taacttagaa cagttccgcc agatttacgc
    32401 ctctggcaag aagtggcaac gatgcgttga agcgattgaa aatatcgaca acattcagcc
    32461 tggcgtcgcc cactccatcg gtgactcatt gacttaccgc gtggagacgg actccgcgac
    32521 cgatgcgcta tttaccgggc atcgacgcta ttttgaagtg cattactacc tgcaagggca
    32581 gcaaaaaatt gaatatgcgc cgaaagagac attacaggtc gtggaatatt atcgtgatga
    32641 aactgaccgt gaatatttaa aaggctgcgg agaaaccgta gaggtccacg aagggcaaat
    32701 cgttatttgc gatatccatg aagcgtatcg gtttatctgc aataacgcgg tcaaaaaagt
    32761 ggttctcaaa gtcaccatcg aagatggtta tttccataac aaataacaac tacggcggca
    32821 aaaggagttt gccgccaccg ctaccctact cattttcgga gatgtgttat gtctgatacc
    32881 aaacgtaata caatcggcaa attcggcttg ctctcgctga cttttgccgc cgtttacagc
    32941 tttaacaacg ttatcaataa taatattgag cttggactgg cctcggcacc gatgtttttc
    33001 ctcgcgacga ttttttattt tattcccttc tgtctgatca ttgcagaatt tgtttcgtta
    33061 aataaaaact cagaagccgg tgtctacgcg tgggtaaaaa gttcgctggg cggacgttgg
    33121 gcatttatta ctgcctatac ctactggttc gtaaacctgt tctttttcac ctcgctgtta
    33181 ccgcgcgtta ttgcttatgc ttcgtatgcc ttcctcggct acgaatatat tatgacgccg
    33241 gttgccacca ccattatcag tatggtgctg ttcgccttct ccacctgggt ttctaccaac
    33301 ggggcgaaaa tgctggggcc aattacctcc gtcacttcaa cgctgatgct gctgttaacg
    33361 ctctcctaca ttttgctggc aggaacggca ctggttggcg gcgtacagcc tgctgacccc
    33421 atcactgttg acgcgatgat cccaaacttt aactgggcgt tcctcggcgt taccacctgg
    33481 atctttatgg ccgcaggtgg cgcggagtcc gtcgctgtgt acgttaacga cgtcaaaggc
    33541 ggttcgaaat cgttcgttaa agtgatcatc ctcgccggga tttttatcgg cgtactgtat
    33601 tccgtctcct cggtgctgat taacgtcttc gtcagcagca aagagttgaa atttactggc
    33661 ggatcggtac aggtattcca cggcatggcg gcgtattttg gtctaccgga agcactgatg
    33721 aatcgctttg tcggtctggt gtcctttacc gcgatgttcg gttccctgct gatgtggacg
    33781 gcaacgccgg tgaaaatttt cttctccgaa atcccggaag gcatctttgg taagaaaacc
    33841 gtcgaactga acgaaaacgg cgttccggcg cgcgcagcgt ggatccagtt cctgatcgtc
    33901 atcccgctga tgattatccc gatgctcggt tccaataccg tgcaggatct gatgaatact
    33961 attattaata tgactgccgc agcgtccatg cttccgccgt tattcatcat gctggcttac
    34021 ctgaatttac gcgccaaatt agatcacctg ccacgcgatt tccgtatggg ctcccgccgc
    34081 accggtatta tcgttgtttc aatgctgatt gcgatatttg ccgtagggtt tgtcgcttcg
    34141 acattcccga ctggcgcgaa tattctgacc atcatttttt ataacgtcgg cggtattgtt
    34201 atcttcctcg gctttgcgtg
//
LOCUS       DAZFRG010000022        33864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFRG010000022 DAZFRG010000000
VERSION     DAZFRG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.022"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..905
                     /locus_tag="ACHKUC_000828"
     CDS             <1..905
                     /locus_tag="ACHKUC_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6617125.1"
                     /translation="QPVLNAKVGGYFNNLRAANQAFVMERRDHAGGDGQTLNLRVIGG
                     RYHYTAVGQLAQHEDTSTVQLSGNLFSGHWGDDGEWMLGIVGGYSDNQGDSRSNMTGT
                     RADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYVWFNNDVSEQDDGVDHYHSSGII
                     ASLEAGYQWLPGHGVVIEPQTQVIYQGVHQDDFTAANHARVSQSQGDDIQTRLGLHSE
                     WRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKQRGEIKVGITGNISQRVSLRG
                     SVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(1045..1767)
                     /gene="ubiG"
                     /locus_tag="ACHKUC_000829"
     CDS             complement(1045..1767)
                     /gene="ubiG"
                     /locus_tag="ACHKUC_000829"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO6617126.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            1914..4541
                     /gene="gyrA"
                     /locus_tag="ACHKUC_000830"
     CDS             1914..4541
                     /gene="gyrA"
                     /locus_tag="ACHKUC_000830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO6617127.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            4690..6378
                     /gene="yfaA"
                     /locus_tag="ACHKUC_000831"
     CDS             4690..6378
                     /gene="yfaA"
                     /locus_tag="ACHKUC_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO6617128.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTQVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            6375..6998
                     /gene="yfaT"
                     /locus_tag="ACHKUC_000832"
     CDS             6375..6998
                     /gene="yfaT"
                     /locus_tag="ACHKUC_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO6617129.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            7022..11536
                     /locus_tag="ACHKUC_000833"
     CDS             7022..11536
                     /locus_tag="ACHKUC_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO6617130.1"
                     /translation="MGTGLANADDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGELVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQKSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKSLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALTLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTKDFSEVDTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            11537..13186
                     /gene="yfaQ"
                     /locus_tag="ACHKUC_000834"
     CDS             11537..13186
                     /gene="yfaQ"
                     /locus_tag="ACHKUC_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO6617131.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQMSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLPTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQQIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKVTEEKST
                     TAVKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            13191..13967
                     /gene="yfaP"
                     /locus_tag="ACHKUC_000835"
     CDS             13191..13967
                     /gene="yfaP"
                     /locus_tag="ACHKUC_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO6617132.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14041..15225)
                     /gene="atoB"
                     /locus_tag="ACHKUC_000836"
     CDS             complement(14041..15225)
                     /gene="atoB"
                     /locus_tag="ACHKUC_000836"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO6617133.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESSALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFE
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(15256..16578)
                     /gene="atoE"
                     /locus_tag="ACHKUC_000837"
     CDS             complement(15256..16578)
                     /gene="atoE"
                     /locus_tag="ACHKUC_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO6617134.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(16575..17225)
                     /gene="atoA"
                     /locus_tag="ACHKUC_000838"
     CDS             complement(16575..17225)
                     /gene="atoA"
                     /locus_tag="ACHKUC_000838"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO6617135.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(17225..17887)
                     /gene="atoD"
                     /locus_tag="ACHKUC_000839"
     CDS             complement(17225..17887)
                     /gene="atoD"
                     /locus_tag="ACHKUC_000839"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO6617136.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(18083..19468)
                     /gene="atoC"
                     /locus_tag="ACHKUC_000840"
     CDS             complement(18083..19468)
                     /gene="atoC"
                     /locus_tag="ACHKUC_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO6617137.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTTPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(19465..21291)
                     /gene="atoS"
                     /locus_tag="ACHKUC_000841"
     CDS             complement(19465..21291)
                     /gene="atoS"
                     /locus_tag="ACHKUC_000841"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO6617138.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            21458..24307
                     /gene="rcsC"
                     /locus_tag="ACHKUC_000842"
     CDS             21458..24307
                     /gene="rcsC"
                     /locus_tag="ACHKUC_000842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO6617139.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(24507..25157)
                     /gene="rcsB"
                     /locus_tag="ACHKUC_000843"
     CDS             complement(24507..25157)
                     /gene="rcsB"
                     /locus_tag="ACHKUC_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO6617140.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(25202..26491)
                     /locus_tag="ACHKUC_000844"
     CDS             complement(25202..26491)
                     /locus_tag="ACHKUC_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6617141.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(26525..26779)
                     /gene="xisR"
                     /locus_tag="ACHKUC_000845"
     CDS             complement(26525..26779)
                     /gene="xisR"
                     /locus_tag="ACHKUC_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6617142.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(26787..26933)
                     /locus_tag="ACHKUC_000846"
     CDS             complement(26787..26933)
                     /locus_tag="ACHKUC_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617143.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(27189..28091)
                     /locus_tag="ACHKUC_000847"
     CDS             complement(27189..28091)
                     /locus_tag="ACHKUC_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617144.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(28088..28312)
                     /locus_tag="ACHKUC_000848"
     CDS             complement(28088..28312)
                     /locus_tag="ACHKUC_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617145.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(28329..28691)
                     /locus_tag="ACHKUC_000849"
     CDS             complement(28329..28691)
                     /locus_tag="ACHKUC_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO6617146.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(28684..29550)
                     /locus_tag="ACHKUC_000850"
     CDS             complement(28684..29550)
                     /locus_tag="ACHKUC_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO6617147.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(29574..30158)
                     /locus_tag="ACHKUC_000851"
     CDS             complement(29574..30158)
                     /locus_tag="ACHKUC_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617148.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(30145..30705)
                     /locus_tag="ACHKUC_000852"
     CDS             complement(30145..30705)
                     /locus_tag="ACHKUC_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617149.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(30878..31072)
                     /locus_tag="ACHKUC_000853"
     CDS             complement(30878..31072)
                     /locus_tag="ACHKUC_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617150.1"
                     /translation="MNNCVEVLDNVHCEITESLSLLQVIIDSGDIGSPDALNMMNCLF
                     RSLSCTADKAEKHLCRTENE"
     gene            complement(31113..31475)
                     /locus_tag="ACHKUC_000854"
     CDS             complement(31113..31475)
                     /locus_tag="ACHKUC_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617151.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGQKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            complement(31650..31850)
                     /locus_tag="ACHKUC_000855"
     CDS             complement(31650..31850)
                     /locus_tag="ACHKUC_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617152.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(31847..32038)
                     /locus_tag="ACHKUC_000856"
     CDS             complement(31847..32038)
                     /locus_tag="ACHKUC_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617153.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(32040..32417)
                     /locus_tag="ACHKUC_000857"
     CDS             complement(32040..32417)
                     /locus_tag="ACHKUC_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6617154.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(32503..33162)
                     /locus_tag="ACHKUC_000858"
     CDS             complement(32503..33162)
                     /locus_tag="ACHKUC_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6617155.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            33502..33795
                     /locus_tag="ACHKUC_000859"
     CDS             33502..33795
                     /locus_tag="ACHKUC_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617156.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         8150 a         8275 c         8759 g         8680 t
ORIGIN      
        1 accagccggt gttgaatgcc aaagttggtg gatattttaa taacctgcgg gcggcaaatc
       61 aggcatttgt gatggagcga cgcgatcacg ctggtggcga tggtcagacg ctgaatttac
      121 gcgttatcgg cgggcgttat cattacacgg cggtagggca gttggctcag catgaagaca
      181 cttctacggt gcagcttagc ggcaacctgt ttagcgggca ctggggtgat gatggcgagt
      241 ggatgcttgg gattgttggt ggctacagcg ataaccaggg cgacagccgc tcgaatatga
      301 ccggaactcg cgccgataac cagaaccacg gttatgccgt tgggctgacc tcaagctggt
      361 atcagcacgg taatcagaag cagggggcct ggttggatag ctggctgcaa tatgtgtggt
      421 ttaacaatga tgtttccgaa caagacgatg gcgtggatca ttaccactcg tcggggatta
      481 tcgcctcgct ggaagccggt tatcagtggt taccggggca tggtgtggtg attgaaccgc
      541 agacgcaggt gatttatcag ggcgtgcatc aggatgattt taccgccgct aaccatgcgc
      601 gcgtgtcaca atcgcagggt gatgatattc agacgcggct gggtttacac agcgaatggc
      661 gtactgctgt tggtgtcacg ccaacgttag atctgaatta ttatcacgat ccccatgcga
      721 cggaaattga agaagatggc agcactatca gtgacgatgc ggtgaagcaa cggggtgaaa
      781 taaaagtggg aataacgggc aatatcagtc agcgagtttc gctgcgcggc agcgtggcgt
      841 ggcagaaagg gagtgatgat tttgcccaga cggcagggtt tttgtcgatg acagtgaaat
      901 ggtaatcgct gtctgaccgg cactgttgcc ggatgcggcg taaacgcctt atcaggccta
      961 cggggagcat ttgtaggcct gataagacgc ggcaagcgtc gcatcaggct tggcgcggtt
     1021 gccggatgaa tccaaacaac aacctcactt attctgcgtg tgcagcatat agttcacatc
     1081 cacgccgggg ccgagcttaa aggtgttagt gatagggttg taatgcagcc cggtcatatg
     1141 ccgttcttta agactggtct gatccaccca gcccagcaat tctgccggtt taataaactt
     1201 cttcacatca tgtgtacctt taggcaccat gcgcaaaata tattccgcac caaccaccgc
     1261 catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt
     1321 caccagttgc gcacaggctc tgaccactga ctgaggatcg gggacgtgct ccagcatctc
     1381 catacaggtc accacgtcat actgtccggc atgttttgcc gcgtgctctt ccacggtttc
     1441 ctgcacgtaa tccacctgaa tgccgctttc cagtgcgtgc agttttgcca cctgcaatgg
     1501 ctcaaagccc atatccagac cggtcaccgt cgcgccctcc cgcgccatac tctcggccag
     1561 aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc cagcacgctc
     1621 ggcaatatag cccagacgca gcgggttaat gcggtgcagc ggtttgaact caccttcaag
     1681 atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt ctacgttgtg
     1741 gtttaccggc gatttttcgg cattcattgg cacttctact ccgtaattgg caagacaaac
     1801 gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac tgcgcggctg
     1861 tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt tagatgagcg
     1921 accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg
     1981 attatgcgat gtcggtcatt gttggccgtg cgctgccaga tgtccgagat ggcctgaagc
     2041 cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg aacaaagcct
     2101 ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc catggtgact
     2161 cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg
     2221 tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca atgcgttata
     2281 cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa aaagagacgg
     2341 tcgatttcgt tgataactat gacggtacgg aaaaaattcc ggacgtcatg ccaaccaaaa
     2401 ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca accaacatcc
     2461 cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat gatgaagaca
     2521 tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg gcggcaatca
     2581 ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag gtatatatcc
     2641 gcgcccgtgc cgaagtagaa gttgacgcca aaaccggacg tgaaaccatt atcgtccacg
     2701 aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag
     2761 aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca
     2821 tcgtgattga agtgaaacgc gatgcggtcg gtgaagttgt gctcaacaac ctctactccc
     2881 agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat ggtcagccga
     2941 agatcatgaa cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt gaagtagtga
     3001 cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat
     3061 tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat gcgccgacgc
     3121 ctgcagaagc gaaaaccgcg ctggttgcaa atccgtggca gctgggcaac gttgctgcga
     3181 tgctggaacg tgcgggcgac gatgctgcgc gtccggaatg gctggagcca gagttcggcg
     3241 tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg gatctgcgtc
     3301 tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa gagctgctgg
     3361 atcagattgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc
     3421 gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtacc gaaatcaccg
     3481 ctaacagcgc cgacatcaac ctggaagatc tgatcaccca ggaagatgtg gtggtcacgc
     3541 tttctcacca gggttacgtg aagtatcagc cgctttctga atatgaagcg cagcgtcgtg
     3601 gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac cgactgctgg
     3661 tggcgaacac tcacgaccat attctgtgct tctccagccg tggccgcgtc tattcgatga
     3721 aggtctacca gttgccagaa gctactcgtg gcgcgcgtgg tcgtccgatc gtcaacctgc
     3781 tgccgctgga acaggacgaa cgtatcaccg cgatcctgcc ggtgaccgag tttgaagaag
     3841 gcgtgaaagt cttcatggcg accgctaacg gtacggtgaa gaaaaccgtc ctcaccgagt
     3901 tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc gatgagctga
     3961 tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgct gaaggtaaag
     4021 tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc ggtgttcgtg
     4081 gtattcgttt aggcgaaggc gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg
     4141 caatcctcac cgcaacgcag aacggttatg gtaaacgtac cgcagtggcg gaatacccaa
     4201 ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt aacggtttag
     4261 tggttggcgc ggtgcaggta gatgactgcg accagatcat gatgatcacc gatgccggta
     4321 cgctggtacg tactcgcgtt tcggaaatca gcatcgtagg ccgtaacacc cagggcgtaa
     4381 tccttatccg tacttcggaa gatgaaaacg tggtgggtct gcaacgtgtt gctgaaccgg
     4441 ttgacgagga agatctggat accatcgacg gcagtgccgc ggaaggggac gatgaaatcg
     4501 ctccggaagt ggacgttgac gacgagccag aagaagaata atttcatcgc ttcatgccaa
     4561 aagggagcca tctccctttt ttgaattgaa aagtccaggc tgtaaagtct gggcttttgt
     4621 cgtattaggg cgcggtaaag tttggctgtg cccgtaaaaa atggctggct atacacaagg
     4681 aatgtggcaa tgagtggtga aaaaaaggcg aaaggctggc ggttttatgg ccttgttggt
     4741 tttggggcaa tagcgctgct atccgctggc gtatgggcat tacaatactc tggcagtggg
     4801 ccagaaaaaa cgttgtcgcc actggtggtg cacaacaatc tgcaaatcga tctcaatgag
     4861 ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct ccttaccatt
     4921 ccgtttctcc acgatgttct gagcgaagat ttcgttttct attatcagaa tcatgccgat
     4981 cgtctgggca ttgaaggcag cattcgccgc attgtctatg aacacgatct cacgctgaaa
     5041 gataagctct tttcgtcact cttagatcag cctgcgcagg cggcgctatg gcacgataaa
     5101 caaggccatc tttcacatta tatggtgctt atccagcgca gtggtttaag caaactgctg
     5161 gagccattgt tgtttgccgc taccagcgac agccagttaa gtaaaacgga aatcagtagc
     5221 atcaagataa atagtgaaac tattcctgtt tatcagttgc gctataacgg caataacgtc
     5281 ctgatgtttg cgacttatca ggacaaaata ttggcgtttt ccagttcgga tatgctgttt
     5341 aaagatgacc aacaggatac cgaagctacg gcgatcgcaa gtgatttgtt gagtggtaaa
     5401 aaacgctggg aaacccgctt tggcctggaa gcgcgtactg ccgaaaaaac gccagtacgc
     5461 cagcgcatcg tagtcagcgc caggttgctg gggtttggct accagcgatt aatgccttct
     5521 tttgctggcg tgcgcttcga aatgggtaac gacggctggc acagttttct ggcgttaaac
     5581 gatgaatccg ccagcgtaga tgccagtttc gattttacgc aggtatggaa cagtatgcct
     5641 gccggagcca gcttctgtgt ggcggtgccg tattcacacg gtattgccga agagatgctt
     5701 tcgcacatca gccaggaaaa cgacaagctg aatggggcgt tagacggtgg cgcggggctg
     5761 tgctggtatg aagactcaaa attgcaaacc ccactgtttg ttggtcagtt tgatggcact
     5821 gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc gcacgaaagc
     5881 aaagcgccag aaggtgtttt gccggtaagc cagactcagc agggcgaagc gcaaatctgg
     5941 cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc gcaacccgat
     6001 caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt
     6061 ttctccctcg atgacacgct ggttaataac gcccttcaag cactgaataa aacccgcccg
     6121 gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa tccacaagga
     6181 atggcgaaac tgctgcgtaa cgaaacgctg accagtctgc cgaagaatct cgaaccggtt
     6241 ttttataacg ccgcacaaac tttattaatg ccgaagctgg acgctttatc tcaacaaccg
     6301 cgttatgtca tgaagctggc ccagatggaa cccggtgccg cctggcagtg gctacccata
     6361 acctggcaac cgctatgaga cacgggctgc tggtgctgat ttgctggctg tattgtgttg
     6421 ttgcccatag tgaaatgcta aatgtcgaac aatccgggct gtttcgcgcg tggtttgtgc
     6481 gcattgcaca ggaacagctc cgccaggggc caagcccacg ctggtatcaa caggattgcg
     6541 cgggcctggt gcgatttgcg gcgaacgagg cgctgaaagt tcacgacagt aaatggctaa
     6601 aaagtaacgg tttatctaac cagtatttgc cgccagagat gacgctaaca cctgaacagc
     6661 gtcaactggc gcaaaactgg aatcagggga atgggaaaac cggcccctat gtgaccgcga
     6721 ttaatttgat tcagtacaac agccagttta ttggccagga cataaaccag gcactgcctg
     6781 gcgatatgat ttttttcgat cagggcgatg cccagcactt aatggtctgg atggggcgtt
     6841 acatcatcta ccacaccgga agcgccacga aaactgacaa cggaatgcgc gcagtcagtc
     6901 tgcaacaact tatgacatgg aaggacaccc gatggatacc caacgattcc aatcccaatt
     6961 tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt ccctgtctct
     7021 tgtgggaaca gggcttgcta atgctgatga ttcgcttcct tccagtaact atgtgccgcc
     7081 cgccggggga acattctttt tgctcgctga cagcagtttt agcagcagtg aagaggcgaa
     7141 agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag agtacggcgg
     7201 cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc agcagaaaaa
     7261 cctgcatcgc attgtggtgc aaccgcaata tctgggcgac ggactgaaca atacgctgac
     7321 ctggctgtgg gataactggt acggcaaatc tcgccgcgtg atgcagcgta ctttctcttc
     7381 tcagtcacgg cagaatgtga ctcaggcgtt acccgaatta cagctcggta atgccattat
     7441 taaaccttcc cgttatgtac agaacaacca gttttcaccg ctgaaaaaat atccactagt
     7501 ggaacagttc cgttacccac tatggcaggc taaaccggtc gagccgcaac aaggggtaaa
     7561 actggaaggc gcatccagca atttcatttc gccgcagccg ggcaacatct atattcctct
     7621 cggcaaacaa gagccgggac tgtacctcgt cgaggcgatg gtgggtgggt atcgggcgac
     7681 gacggtggtg tttgtttccg ataccgtggc gcttagcaaa gtgtcaggta acgagcttct
     7741 ggtgtggacc gcggggaaaa aacagggtga agcgaagccc ggctcagaga tcttgtggac
     7801 tgacggtctt ggcgtgatga cccgcggtgt gaccgatggc agcggtacct tgcagttaca
     7861 acatatatcg ccagaacgtt catatattct gggtaaggat gctgaaggcg gcgtttttgt
     7921 ctccgagaac tttttctacg aaagcgaaat ctacaacacc cgcttgtata tctttaccga
     7981 tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca
     8041 cgatccgttg cattcatccc ccatcgtcag cgcaccggcg aagctttcgg tgctggacgc
     8101 caacggcagt ctgttgcaaa ccgtcaatgt cacgctggat gcgcgtaatg gcgggcaggg
     8161 gagtttccgc ctgccagaaa atgccgtcgc cggaggttat gagttacgtc ttgcttaccg
     8221 caaacaggtc tatagcagca gttttcgcgt ggcaaactac atcaaacccc attttgagat
     8281 tggtttagct ctcgataaaa aagagttcaa aactggcgaa gcggtcagcg gcaaactgca
     8341 actgctctac ccggatggcg agccggtaaa aaatgctcgc gtgcagttaa gtttgcgcgc
     8401 tcagcaatta tcaatggttg gtaacgattt gcgttatgca ggacgtttcc cggtgtcgct
     8461 ggaaggcagt gaaacggttt ccgacgataa tggtcatgtg gcgttgaatc tccccgccgc
     8521 cgataaaccg agccgttatt tgttaaccgt ctccgccagt gacggcgcgg cgtatcgagt
     8581 taccaccacc aaagagatcc tcattgaacg tggcctggcg cattactctt taagtaccgc
     8641 cgcacaatac agtaatagcg gcgagctggt tgtgttccgt tatgccgcgc tggaatcttc
     8701 aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga gccacagcgg
     8761 agagctaccg tcaggcggca aatcctttac cgtcaatttc gctaaacctg gcaactacaa
     8821 tctgacatta cgcgataaag acggcttaat tctcgccggg ttaagccatg cagtcagcgg
     8881 taagggcagc acgtcgcata ctggtacggt agatatcgtg gcagataaaa cgctgtacca
     8941 gcctggcgaa accgcgaaga tgctgattac cttcccggag ccaattgatg aagcattatt
     9001 gacgctggaa cgcgatcgcg tggaacagaa gtcgctgcta tcgcatccgg caaactggct
     9061 aacgctacaa cgtttaaacg atacccagta tgaagcccgc gtaccggtga gcaattcctt
     9121 tgcgcctaac atcacttttt cggtgctgta tacccgtaac ggtcagtaca gttttcagaa
     9181 cgccgggatt aaagttgccg ttccccagct ggatatccgg gtgaaaacgg acaaaaccca
     9241 ttaccagcct ggtgaactgg tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt
     9301 ttctgcgcag ttaacggtag gcgtggtcga tgaaatgatc tacgcgctgc aaccggaaat
     9361 cgcgccgaat atcggcaaat ttttctatcc gctggggcgt aacaatgtgc gtaccagctc
     9421 cagcctgtca tttatcagct acgatcaggc gctctccagc gagccggttg cgcctggcgc
     9481 gactaaccgc agcgagcggc gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga
     9541 taccgcggcc tggatgccgt cactcacaac cgataaacaa ggcaaagcgt atttcacgtt
     9601 cctgatgcct gattcgttaa cccgctggcg catcaccgcg cgtgggatga acggcgacgg
     9661 gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa agtctctaca tgaaatggag
     9721 tatgccaacg gtgtatcgca tgggcgacaa accggcggca ggactgttta tcttcagtca
     9781 gcaggataac gaaccggtgg cgctggtgac taaatttgca ggtgctgaga tgcgccagac
     9841 gctgacgctg cacaaagggg cgaattatat ttcgctgacg cagaatattc agcaatctgg
     9901 cttgttaagt gcagaactgc aacaaaatgg gcaagtacag gacagcatta gcacaaaact
     9961 gtcttttgtg gataacagct ggcccgttga acaacagaaa aacgtcatgc tcggcggtgg
    10021 cgataatgcg ctgacgttgc ccgagcaggc gagcaatatc cggctacaaa gtagcgaaac
    10081 gccgcaggag attttccgta acaatcttga tgcattagtc gatgaaccgt ggggtggcgt
    10141 gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca
    10201 tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc tgcggctgat
    10261 gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca atggcgacgc
    10321 cttcctcacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac
    10381 gcagcaaccg gaatactggc agcatatgct cgacagctac gcggagcagg cggataacat
    10441 gccgttattg catcgggcgc tggtgctggc gtgggcgcag gagatgaatc tgccgtgcaa
    10501 aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaaa ctaaagattt
    10561 ctctgaggta gacaccagcg atatcaatga cagcatgatc ctcgatacac ctgaatctcc
    10621 actggcagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa
    10681 gtccactgtg atgccacagg ttcagcaata tgcgtgggat aaagcggcaa acagtaacca
    10741 gccgctggcg cacacggttg tgctgcttaa tagcggtggc gacgctaccc aggcggccgc
    10801 tattttaagt ggtttgaccg ctgagcaatc cactattgag cgcgcgctgg ccatgaactg
    10861 gctggcgaaa tatatggcga cgatgcctcc agttgtgttg cctgcgcctg cgggcgcatg
    10921 ggccaaacat aagttaactg gagggggcga agactggcgt tgggttggtc agggtgtgcc
    10981 ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga
    11041 accggcaaaa acggctcaac aaagtaacat tccggtgacc gttgaacgcc agttgtatcg
    11101 gcttatcccc ggtgaagaag agatgagctt tactctgcag ccggtgacca gcaatgagat
    11161 tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg atgcagttct
    11221 gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc gcacaacatg
    11281 gggcatttcg gtcaataaac ctaacgctgg aaaacagcag gggcaattgc tggaaaaagc
    11341 gcgtaatgaa atgggcgaac tggcctatat ggtgccggtg aaagaactga cgggaacggt
    11401 cactttccgc catttgctgc gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg
    11461 ttatgtgcgt tcctatgcac ctgcacagca aagtgttgcg gcagggagtg aatggaccgg
    11521 gatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt tggttactct
    11581 gccaacgctt gcggaggaga cgccgttaca actggtgctg cgcggtgcgc agcacgatca
    11641 actgtatcag atgtcgtcat caggcgtgac caaagtctca gcattacctg acacgctcac
    11701 cacgccgtta gggagtttat ggaaactgta tgtttacgcc tggctggagg atacccatca
    11761 accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc
    11821 tggggagagc ataacgcgcg ataccgcgct ggtacgctcc tgtggactct attttgcgcc
    11881 gcaacggttg catattggtg cagatatgtg ggggcaatac tggcaacagc gtcaggcacc
    11941 tgcgtggtta gcgtctcttc cgacgctgaa accagaaacc tcggtaacgg ttaaatcgct
    12001 gcttgattca ttagcgacgt tacccgcgca aaacaaagct caggaagtct tactggatgt
    12061 ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa
    12121 aacctggagc tggtttgccg atgataaaca gcaaatccgt cagggtggtt ttgccggctg
    12181 gttaaccgat ggtacaccgc tgtgggttac tggcagtggc accagcaaaa cggtactgac
    12241 ccgttatgcg acggtgctta atcgggtatt gccagtgccg acgcaagttg ccagtgggca
    12301 atgtgtagag gtggagctgt ttgcccgtta tccgctgaag aaagttactg aggaaaagag
    12361 tacgacggca gtcaaaccag gcgtgctcaa tgggcgctat cgtgtgacgt ttgccaatgg
    12421 taaccacatg acatttgtca gccacggtga gacaacgtta ctgacggtaa agggcaaact
    12481 gaaattacag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc
    12541 gaaaagtacg cctcctgagg cagcgaaagc catgaccgtg gcgattcgca cgtatttaca
    12601 gcaaaacgct gatcgcgatg gtgattgcct gagcatcccg gacagtagcg ctacccagcg
    12661 cgtttcagct tcgccagcca ctgtaggcgc gcggacgatg accgcctgga cgcaggatct
    12721 catctatgcg ggcgatccgg ttcattatca tggcagccgg gttaccgaag ggacgctttc
    12781 ctggcggcac gctactgccc aggcggggca gggagagcgt tacgatcaga tacttgcgtt
    12841 tgcttatcct gacaacaacc ttagccgctg gggagcgcca cgctcaacct gccagttatt
    12901 acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta tattacaagg
    12961 tgagacggga tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct caggtttccc
    13021 ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc ttcaggatcg
    13081 gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca ccgggcttga
    13141 tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct atgcgaaaga
    13201 tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc tcacagtgaa ggtgtgcagg
    13261 aagtcgagat tgatgcaccg ctctctggct ggcatccggc agagggtgaa gatgccagtt
    13321 tttcacaaac gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct
    13381 cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc gggtaaagtt cagcagggcc
    13441 ggctggtggt caacggtgcc agtatgccgc agagaattga atcagatggt tcttttgcac
    13501 gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagccca gatgggcaaa
    13561 gccgacaaaa aatgcagttt tactcaacgc cgggtgctgg ggcgattcgc gcacgtttac
    13621 ggctggttct ctcgtgggat acggacaata ccgacctcga tcttcatgtc gttacgcctg
    13681 acggtgaaca tgcctggtac ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg
    13741 atgtcacgac agggtacggc cccgagattt tcgccatgcc agcgccagtt cacggccgtt
    13801 atcaggtgta tatcaactac tatggcggac gcagcgaaac ggaattaact acggcccaac
    13861 tgacgctgat caccgatgaa gggtcggtca atgagaaaca ggaaactttt attgtaccga
    13921 tgcgtaatgc tggcgaactg acgctggtga aaagttttga ctggtgatta atgaggttat
    13981 ctttactgtc aatgttcaag aaaacgcccg atgactttcg ctatcgggcg tttttacatg
    14041 ttaattcaat cgttcaatca ccatcgcaat tccctggccg ccaccaatgc acagtgttgc
    14101 cagccccagc gttttatcgc gtgcctgcat tgcatgtaat agtgtgacca gaatacgagc
    14161 accactggca ccgataggat gccccagcgc gatggccccg ccgttgacat tcactttctc
    14221 aggctcaaag cccagggttt tcccaacggc aaggaactgt gcagcaaatg cttcattagc
    14281 ctcaatgaga tcaatatccg ccagttgcag ccccgccagt tgtaacgctt tttgcgtggc
    14341 aggtactggc cccataccca tcaatgcggg gggcacgcca ccgctggcat aacttttaat
    14401 gcgagccagg ggattcaggc ctgctgccag cgccgaagat tcttccataa tcaccagagc
    14461 ggcagcaccg tcgttaatgc cagacgcgtt cccggcggtg actgttcctg ctttatcgaa
    14521 ggccgggcgc aatgcgccta acgcttcagc cgtagaatcc gctttcggga attcgtcttg
    14581 actgaagacg aaggttttct tccgagtgac aacatttacc gggacgattt cggctgtaaa
    14641 agcaccggac tcaattgcgg ctgccgcttt acgctgtgaa tgtagcgcca gttcatcctg
    14701 catttcacgg gtaattccgt actctttagc cacgttttcg gcggtaatcc ccatatgata
    14761 accatgggtg gcgcacatca gaccatcgcg caggattacg tcataaacct gtccgtctcc
    14821 aagacgataa ccagagcgtg cttttgcatc gagtaagtag ggcgctaaac tcatattttc
    14881 catacccccc gccacaatgc tctgcgcctg acctgcctga attgcctggg cggcaagcgc
    14941 cacactttta agacccgaac cacacacttt attgaccgtg aatccgcaca ccgtttcagc
    15001 cagtccgctt tttagcagcg cctgacgtgc cggattttgc cccagtccgg cttgcaacac
    15061 gttacccata atcacttcat caacgtgtag tgaatcgatt tttgcacgtt caatggcggc
    15121 tttaattact gtcgccccca ggtcgatggc gctggtggaa gcgagtgaac cgttaaaact
    15181 accgatagca gtacgtaccg cactgacgat gacacaattt ttcattttat attccttctg
    15241 tctgtagggt tgccgtcaga acagcgttaa accaatgacg aaaatgacgc cggagaagag
    15301 cagggcagtg atgcaatagc ccatgatgtc gcggacaccg agtccggcga ttgccagtgc
    15361 tggcagcgcc cagaacggtt gtgccatgtt catccattgc tcgccgtagg cgatcgccat
    15421 taccgattta ccgagatcag cgcccagcgc ctgggctgcg ggcatcacga acggcccctg
    15481 aataacccag tgcccgccgc cagacggaac ggcgaagtta atcagtgcag aactaaaaaa
    15541 ggtcatgacc gggaaggtgt ctttgttcgc aacattgatg aagaattcgg taatgagtcc
    15601 gcccagaccg gagtgctcca tcatcagttg gatccccgcg tagaagggga attgcaccag
    15661 aataccggcg gtactgcgtg ctgccgcgct gatagcacgc atataagcca ttggcgtttt
    15721 atgtagcagc agacccgcaa tcataaacat caggttgacg gtattgatgg tgatgttgaa
    15781 gccatgttcg ctgaagtaca tcgccaggta agcgataccg agtgcgccga tgatcaacgt
    15841 cagaatacgg ctttcttcca gtcgctcgga cggtggagca tctttcggta gttgcttttg
    15901 aaaatcagcc tcttccatga gtagtttcgg atcgatactc accacgtcag acggttttgg
    15961 catcatcatg cgggtgataa atggcatcac cacaatcaac gccacagtga tgaaaatatt
    16021 aaaaccactg aacagagtat cgcccaccgg gatcagcccg gcgatatgct caaccgggtt
    16081 gcccggtgtt gcagccaaca gaggcattga gccagagaaa ccgccacccc aggtgagaaa
    16141 accaatgtag gcgcaggcaa tgagcaacgg ataatcagaa ccgggtactc gccgtgcgac
    16201 ttcacgggcg aacattgcac cgacaaccaa accaaatccc cagttgatga cacaagcaac
    16261 tgaaccgaag aaagtaacca gcatgacgcc ctgtacgggc gtctttgcgg cggaggcggc
    16321 agtacgcagc aaacttttca ccggcgcgga gctggcaagg gcatgaccgg taacgatgat
    16381 aagcgccatc tgcataccaa acgccagcaa gttccagaaa ccgtcacccc acattttcac
    16441 catgctgatc ggcgtttgtg gtgttaacca aagcgcgatc acgaatgtta gcaatgtcag
    16501 caacatggca aagatcagtg gatcgggaag ccatcggctg acaaaacgcg tcataaaacg
    16561 cgatatgcga ccaatcataa atcaccccgt tgcgtattca gatcggcagc gacttcaaac
    16621 cgagcttctg ttttggcacg cacggtggct aaatcacacc cgtcggcaat ttcggtgagc
    16681 cacattttgc cgtcaataaa acgaaagaca gccagttcag taaccagcat atgcaccgca
    16741 tgttgcgcag tgagtggcat ggtgcagcgg cgcaaaattt ttgctgaacc atctttggcg
    16801 caatgttcca tggcgatgat cactttgcgc gacccggtca ccagatccat cgcgccaccc
    16861 ataccgggca ccattttccc aggcactacc cagttcgcga ggtttgcttc ttcgtctact
    16921 tgcaaaccgc cgagcacgca ggcatcaata tgaccgccac ggattagcgc aaatgacatg
    16981 gcgctatcaa acatggctgc gccgggtaaa atgccgcacg gttgtccgcc agcgttcacc
    17041 agatctggat gcgctgttgt gaccgggcct aaaccgagga agccgttttc tgattgcaga
    17101 gtgatatgaa taccctccgg taaataattg gcgaccattg tgggtaaacc gatccctaag
    17161 ttaacgatgt caccatcacg aagctcttgc gccacacggc gcgcaatacg ttgtttcgca
    17221 tccattattt gctctcctgt gaaacgatga tgtggtcgat aacggcacca ggggtgacaa
    17281 tatggtcagg ttgcagctcg ccggtttcga ccagttcatc cggctctacc agcgtgatat
    17341 cagccgcaag ggctatcagg gggttaaagt tgcgggcgct aaggtgatag gtcaggttgc
    17401 caagtgtgtc gcaacgatga gcgcgaatta gcgccaggtc ggcgcgcagc gggcgttcga
    17461 gcagccaggt tttaccgtcg agtgtcagtg tctgtttgcc ttcctctacg acggtgccga
    17521 caccagttgg ggtgagaaaa ccaccaagtc cagctccgcc acagcgaatt tgctcgatta
    17581 gcgtaccttg cggcaccaga acgacgtcca tctcaccaga tatcatgcgc cgacctgttt
    17641 ccgggttggt gccgatatgt gaagcaatca ctttgcggac tcgaccattg acgatgagcg
    17701 gaccgatgcc tgtatcaaca aacgcggtat cattggctat caatgtcagg tcgcgaacac
    17761 cagattccag taatgcttca accaggcggg atggagtgcc aatccccata aatccgccca
    17821 ccatgatggt catgccgtca cgaaagaagc cggtggcgtc ttgtaatgtc atcaattttg
    17881 ttttcatttt ttatccctct tgcatacacc gttgtgggta cagtcaggga atagcaaggg
    17941 gagtgccaga atgagattat tgaatgttgt tatttaacta attgaataga aaaggataaa
    18001 ttagatgggg attcataagg agaatgagcg gctatgcaga aaattgcgca ctgtgcaaat
    18061 ttctgcatag caagttttgg cgttatacat ccgccggatc gataccgtat tcctggagtt
    18121 tatacatcaa tgcacggcga ctgatgccca gcattaaagc agtgcgggtt cggtttcctt
    18181 cttgttgttc cagcacttcc ataatgatgc gtttttcgac gcgtttaatt tcctctttta
    18241 aattacgctc accgacaggg gttgtttttg cttcaccagc attacagact ggctgacgaa
    18301 tctgcggcgg aagatcctca gaaaaaatga tcgggcctga attcatcacg acagcgcgtt
    18361 caataacgtt ggaaagctct cgaatatttc ccggccatga ccaggcggta agcagtgaca
    18421 ttgccatcgg atcgatgtcg ataatatcgc gctgattctc actactgaat ttttgcaaaa
    18481 agtgattagc taacagggaa atatcttccc ggcgatcgcg cagaggcggc agtattaaat
    18541 gaataacgtt aaggcgataa aagagatctt cacggaaggt gccttctttc accattgcct
    18601 gcaagtcgcg gttggtggca gcaatgatgc ggatatcaac ttttatggtc tgatggccgc
    18661 caatccgttc aaattcccgt tcctgtagaa tgcgtagtaa tttggcttgc agtaccagcg
    18721 gcatttcgcc aatttcgtcg aggagcagag taccttcgtt ggcacgttca aataatccct
    18781 gacgcaaggt ttgtgcacca gtaaatgcac ctttttcatg accaaacagt tcactttcga
    18841 gcaacgattc cggcagcgcc gcgcagttga ctttaatgaa cggccccttt gcccgccgcg
    18901 aattgtagtg aatcgctctg gcaatcaact ctttcccggt gccgctttca ccgctaatca
    18961 agacgctggc ctgagaaagg gcaattttgg cggtgtcttt gcagatgtcc atcatcgccg
    19021 ggctgttggt gagaatgtgc ccccattgcc agctggtgct cagtgcctgg tgcagatgac
    19081 ggatctcttt tttcattgac tggagttgta aagcgcgctg aacgattaaa ttcaactcat
    19141 cgagatcaaa cggtttgata acatagtcga aggctccgca gcgtagtgct tcgacggcgg
    19201 tttccacttc agcataggcc gtcatcagaa taacgggtgt ccgggtctca tggctgcgca
    19261 tctcctttag tgccttgatg ccgtccatct ctggcatacg gatatccatc aacaccacgt
    19321 cagggtgaat atcggcaaac aggtgtaatg ctgtgcgccc gttgttcgca caatgtgttt
    19381 cgaatccttg tagtgcaaaa gcggtgctca gcatacggcg aacattatct tcatcatcca
    19441 caataaggat gcgattaata gcagtcatac agtctgattt ccctgcgggt tgatcggtaa
    19501 aataagcgtg aaggttgcgc cgtagcccgg caaactggcg acgcgaatat caccctgatg
    19561 ggcattaatg atgcgttgac ttaacgccag accaagcccg gttcctgagg ctttggtagt
    19621 gaaaaaggga tcgaagatct ttttttgcag cgagagatca atgccactgc cgttgtcctc
    19681 tatcgaaatg gcctgttgcg agtcgctata ctgccaggtt cgaatgcgaa ttttccctcg
    19741 tgcactgata gcctggacgg cattgatcag gatattcagt agtacctgtt tgagcagttc
    19801 acgatcggcg ttaatcgggc ttaattcatt atccagttcg cttatgaagt cgacccgcgc
    19861 ttgtacgccg gcggtttgta ccagtaccag agtttcttca accaatgcat tgaggctgac
    19921 ttgttgccat tgactgtggc gtggacgtga aaattcgagc aattgctgaa taactttgtt
    19981 aattgaatcg atttctttga gtactacgga cagatattcc tgatgtattg ggtcacgggt
    20041 ttgttggcgc aagatctgta cataaccacg aatagccgtt aacggattac gtacttcatg
    20101 cgccacgcca gccatcagct cacccagtgt ggcgaggcgt tctgcttgcg ccatgcggcg
    20161 ctgggtttct ttgcgggcag ttaaatcaga gaaaatcacc aaagcaccta tcatttcacc
    20221 gtgcgtgtta tgaatacgac tggtcgtgac actgagttca atggtgcggt cacggcctgg
    20281 aaaactgatc tccagtgcta catgttcggt gccatgttcc agcgtatcca gtacgggact
    20341 gtagaactga gtattgtcga acaacatgga gtaaggctgc cctaccagtt catgacgttg
    20401 atagccagtg atgacttctg ctgctgggtt catggtggtt acatcaccct ggcggtcaat
    20461 ggcaatgacg ccatcggcag cgttttcaat aatcagatcg ttaagtgttc gcgtttcacg
    20521 cagtgcctgg gcgaggttgt taacactctg actgatttgc cccatttcac cgggcaattg
    20581 tggtaatcga gtgggaatat tttgagccag agtcgagagg ccatcggtga tgatatctat
    20641 attggcgctc aggcgacggg agaaaaggac aatcaacagc aggcttatca gcaaaccggc
    20701 ggtgagcaca atgataatcc tcacatccat tttccaggcc tggcggcgaa tatcttcggt
    20761 taattcattg gcccagatat agccgaggat ttcaccatta cgctcaatgg gaatcattga
    20821 attcaaaata tcgccgcgca cctgcctgcc tgaataaacc aaaggggtat ttgtacgcat
    20881 gacttcgcga ccagggtgat ctgcggcaat ggtaacgccc acattattct gatatagcgc
    20941 tgaaggcgcg taggtgatta tcgcatccag cgttttgttg taataaccag cgccgatgcc
    21001 agggaaggcg tgagtgatat tttcggtaat gggggcaagt tctgcattta atgcgcggat
    21061 acgctcctca cgcggtaagt cgatgtagag atcgtagcga ttgcctagcg cctgattaag
    21121 caggttgacc acggcagaaa gttttttctc tttttcagat aagactgctg aacgtccttc
    21181 cgtttctacg atataaccaa tagtaagcgt tgggacaatg accatcagga ttgccatcag
    21241 gatcatttga ttgcgtaagc ggcgtggata aatccacttc atataatgca tgctgaaatc
    21301 cactagtctt gtccggtata tgacgattat cagaggttaa ggtgatgatt tctcggcggt
    21361 gtatcatatt ccagagaaga gagaacattg cggtaacacg cttttaccgc taccttaacc
    21421 acactccatc ggtcacctga ggcggagctt cgcccctttg aaataccttg cttcttttcg
    21481 tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc tctggctgtt
    21541 gattgctttt tcatccgttt tttacatcgt taatgcgtta catcagcgag aatcggaaat
    21601 tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac gcacctctga
    21661 tgtgatgaaa gagctgaagt acatagctga aaatcgctta tcggcagaaa acggtgtgct
    21721 ttccccgcgt ggacgagaaa cgcagacgga tgtgcctgcg tttgaaccgc tatttgctga
    21781 ctctgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat tggcgtggtt
    21841 tatgcgctac tggcgcgata atttttctgc ggcttacgat ctaaaccggg tatttttaat
    21901 cggcagcgat aacctctgca tggctaattt cggtctgcgt gatatgccag tggaacgcga
    21961 taccgcgtta aaagctttgc atgaacgcat taataaatat cgaaatgcac cacaagatga
    22021 tagtggcagt aacctctact ggatcagcga aggtccgcgc cctggcgtcg ggtattttta
    22081 cgccttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg tcgagcaaac
    22141 catccggatg gagaactttt tcttacctgg tacgttgccg atgggggtta ccattcttga
    22201 tgaaaatggt cataccctga tttcgcttac cggaccagaa agcaaaatta agggcgatcc
    22261 tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccgcg aactggtgct
    22321 gaagaaaaat ctgccaccct catcgctaag catcgtttat tcagtgccag ttgataaggt
    22381 gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc ttgccggagc
    22441 tgcattgttt actctcgcgc ggatgtacga gcggcgtatt ttcattccgg cggaaagcga
    22501 cgccctgcga ctggaagaac atgagcagtt taatcgcaag attgtcgcct ccgcgccagt
    22561 gggtatctgc attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg aactggcgca
    22621 tacctatctc aatatgctta cgcatgagga ccgccaacgg ctgacacaaa ttatctgtgg
    22681 gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc aaatcagctt
    22741 cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg atgtttcttc
    22801 gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac aggcgagcca
    22861 gtcaaaatcg atgttcctgg ccaccgtcag tcatgagctg cgaacgccgc tgtatggcat
    22921 tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg atcgtctggt
    22981 gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata ttctcgattt
    23041 ctcgaaaatt gaatcagaac agttgaagat cgaaccgcgt gagttttcac cgcgtgaagt
    23101 gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt taggcttgta
    23161 ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc gtttacaaca
    23221 ggtcatctct aacctgctga gtaacgccat aaaattcacc gataccggct gtatagtttt
    23281 gcatgttcgc gctgatggcg attatctctc tatccgtgtt cgcgataccg gcgtggggat
    23341 accggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggtcggaa cgggcgtaca
    23401 gcgtaacttc caggggactg gtctgggtct ggcgatttgt gaaaaactga tcagcatgat
    23461 ggacggcgac atctcggtag attcagaacc gggaatgggc agccagttta ccgtgcgtat
    23521 tccgttgtac ggcgctcagt atccgcagaa aaaaggcgtg gaagggttga gtggtaaacg
    23581 ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca gtttgcagcg
    23641 cagcggcatc gtcgttacaa catacgaagg gcaggaaccg actcccgaag atgtgttaat
    23701 cactgacgag gtagtgagta aaaaatggca gggcagagcg gtagtgacct tctgtcgtcg
    23761 ccatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg tggctgctcc
    23821 gcatgagcta ccggcattgt tggcgcgtat ttatttgatc gagatggaga gcgacgatcc
    23881 tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aatgacgata tgatgattct
    23941 ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg gatcgttggg
    24001 ctatcaatgt aaaaccgcga atgatggcgt cgatgcgctt aatgtactta acaagaatca
    24061 tattgatatc gtgcttagcg acgtcaatat gccaaatatg gatggttacc gcttgacgca
    24121 acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta atgcgttggc
    24181 tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctgtcga agccagtaac
    24241 gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga aatcgcggga
    24301 atcgtaggac ggataaggcg tttacgccgc acccggcaat ccgtgcaaat gccagataag
    24361 acactgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat gggaatcgta
    24421 ggacggataa ggcgtttacg ccgcatccgg caatccgtgc aaatgccaga taagacacta
    24481 acgcgtctta tctggcctac aggtgattag tctttatctg ccggacttaa cgtcacagaa
    24541 gagagataat tcagcagagc aatatcgttc tcgacaccca gcttcatcat cgcagatttc
    24601 ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc ggtcaccagg
    24661 aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg cttgtcacca
    24721 taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt aaatttcttc
    24781 cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg tttcagcacg
    24841 atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt gttcatggtc
    24901 agaacaatga tcgacaggct tgggaaatgg cgcttgatgt acttgattaa ggtaatgcca
    24961 tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgtgc atccagtttc
    25021 ggcaagttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt cacccactca
    25081 atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc ggcaataatt
    25141 acgttcatat tgttcatgta cttgcaccta tgtattaact atttgatttt aaaaatatat
    25201 tctatgcttt gtttagtcgt tggggcacct gtgggacata agtacatagc tttctgttta
    25261 gtacttctac ctgggactgg ttattgtctt tcatccatgc accgtaaaca ttgtaaacca
    25321 tctgggcatt cgcatgtccc atctggttgg ctatgaagtt tggatttgct ccggcagtta
    25381 aagcccagca agcataagta tgccgagact gatacgcttt tctatggcga atttttgctc
    25441 gttttaatgc agattcccat gttctgttga tggatgttac ggcataatga actcctgagt
    25501 tacctgtgcg atctaatatc tgaggattga aaacaaagct gcatgagtga gtgcttgtcc
    25561 ggccatactc tcgcagcttt acttctatct gatattgttt accaagcctg gttaatgtcg
    25621 cctgattttt taatacgtca attgctggct gtatgagttg tattaccctg tttgttcctg
    25681 catctgtttt gggtagtgta aattcctttg tctgagtgta attccttttg acggtcaagg
    25741 tacccgcttc cagatcgata tcctcccatg ccagcccaca cagttcgcca tgccgcatcc
    25801 cggtatatac agccagcgac cagaaatttt tagtttgctg gtggtggcac gcatcaatca
    25861 gtctggcaaa ctcatctttt gttagtggat cgggaggtgt tttcgctctt ttcagtgatg
    25921 gaagctcact gaaagggttt ttgcctatat acccattgtt tgccgcaaaa cggaacatcc
    25981 cggagataat tgtcatatag ctatttacag taggtacact acgaccttta ataggcttcc
    26041 tctgattttt tcctggcaac tgatatcccg ttaataattc tttacgaaaa atgagaacat
    26101 cttcctgtgt aattgatgat gccagtttgt tccctccaag gcagtcaagg caatttcgga
    26161 tagctgattt gtatctgacc atggcatttg ctgttatttc cattttctta agctcaagcc
    26221 atttttccga aagtgcctta atggttatct ctttttttcc cagaccaaaa tgtttcaggt
    26281 taggggaatt agggaactgc gcggcgtagt cgaagctccc cattcttatt gcaaaacaaa
    26341 cggaagtgcg aagttcacct gcgatcttcc ggtttttggc ggtgtcagga acaccgaggt
    26401 tttctctgac acgtttgccg ttatagtgaa accatatgcg gagtgatccg ccatggtttt
    26461 caacgcctgt cgggtatgat gcgttactca ttaaacctcc cagacgtcca ggagcattaa
    26521 caggttaacc ggaacttgca tttttggcac ctggttgttt ctggttttcg atccagcgca
    26581 taatttcctc aatgttgtat acacattcac tgaaaggccc gggctctcct tctgctgcgt
    26641 aatgacggta ttctttcccc agtaaccagg atttttttct tgctctggca atggttcctg
    26701 atttcagtcc agttgcagca ataagggttc tttctgtgca ccatttgctg ggcgttatct
    26761 gatagatgat tgtttccatt cctgactcac actatcttca ggccacggca gtggcaccac
    26821 acgtcaaaca ttcgctttac aacttcacgg cagtagaagc cgtgagcatc tctggttaaa
    26881 tcgtatcggt acccgtatct gagtcgtatc catatttcaa actcgcgatt cacgacttat
    26941 tctcctgctg tctttatgcc agttcattcg gttgcgctgg ggccctggcc ttccgtggcc
    27001 tgtatttgcg gcgttttctg gcttcaatgc gtatctttgt caccctgact ggctttggtt
    27061 cgtgccggaa cactttgttt ctgatattac gccgtgtatt catcatccag ataaaacgct
    27121 gggtatggtc gcatccgtca tcaacggtaa ttactgtgcg gaccagttca tcgttaatat
    27181 caaccatttc acccattcac tgagccttca tacgcgaatt tcgtggcttc catcagttgc
    27241 gtccataccc attcaagaaa ttgtgcgttt tctcctgagc ggtcatattc atcgacgctg
    27301 cggggcttga tggtcgccag tagtggcaca atccggtact tcatctgttc ggttaacttg
    27361 tccggtacaa gtcgccagcc gttatatttc gatggaacat ggtggtattc agccaggaag
    27421 cgtcgtatat cgtttttcat ttcctcgcat gagttctgat catgaagtgc agcggaaagc
    27481 cagcgtccga tatattcagg gattcgcaca tcaagtactt tttctgccgt cgtcagcatt
    27541 gcctgtcggc aggcgttcca gccgttgaca tagtgacgtg ttgcatccca tgaaacctgc
    27601 acatcagggt aagcatcctg ttctgttctc actggcggta tctgaggaac agtgaatagt
    27661 ggactgacga tatatttatc cagagagcct ccccgctctt ctgcaagact ttcccacgat
    27721 gtcattgtgg ggaccctgta acctgctttt ttgtgcgtat acatccatgc cactctgccg
    27781 ttatcatctg cggcggcaat gcgctgccag gcaggataaa gcggggtgta tgtttctctg
    27841 tcattacggg cgtcaggcat ggcatcaata ctgcaaactt cccccgtaag gttattcagc
    27901 caggccttcg ggctaatctc tgacgttacg atttcatcca gttttttctc cagattttct
    27961 atgtgactga ttagcgcaag gataactggc ggagtggcaa gggagtgaaa tgcttccaga
    28021 tcacatccct tgtcgccata tacggtattt tgtgcggcgt cacgcagtgc tttcagatca
    28081 gcttctttca tcgttcagac tcccttgttg ctgttttgta ggcgcgcagg atgtcgcgtg
    28141 tttttcctga taatgacgac ttaaccagga aaaagccgct ccggctttct gtgatgtccg
    28201 gcgtgcacag aagtaccgca tcaacgacac gattatgttt tcgcaatgtc aggcgggaac
    28261 tggtgataat cagttttgcc tgttcgccgt aatcggtgaa tgaaataatc atctgatggc
    28321 gatctccgtt atgaaagtgt tcctgctggt tcaatgctga taatttctgc tgcaataagc
    28381 atacctgcca gccatgcttc gccgagcatg tcctcatccg tacatgcaag atcaccgatc
    28441 tgtattgtgg ccataatgtt agtttcaccg gtatattcat cctgtgcttc ttcatacggc
    28501 aggtcttcat aaaggttttc aattgcgcag gttattacat ccagtcccgt cagattgccg
    28561 acaacgtcca cttcaaatgt ttcgcggtac tcccattgcc cgagtgtcag acgaaccgtc
    28621 tgtcttgcca tgcgcccgca taatgtcatg ttcgggtcgt agttcatgat tgccgcagta
    28681 gtgctattca tgttctgcct ccggtgtgta aatagctttg tcgtgtttaa attcaccgtt
    28741 ccagtttttc ttcataggga gttcaccgtt catgtaaaac tggtataaac ggtggcatcc
    28801 tttttccagc aatacggggg tgaatttcgt gaatgttgct ttcccgtgtg gagtgatctg
    28861 tactgcttct tcggtcaggt atctgtcacg ggcataagag gccgtgcgcc agcggggtgt
    28921 tttctccgga tcgcgttgtt cgttgaatac ccattttcgg ccagacagcc accacattat
    28981 ctggctggta tttacgccgt tcagcgcacg gcagaactgg ggaacagtta tgcctttaga
    29041 gaaatgtttt tccagactgt ctacggttgc agagagcgtt ttattttcca gggccatagc
    29101 ttcagcccgt tcttcggcct caataaccat caacgcgagt tccttgcgag tgagtacgcg
    29161 ctgaacatcg atgttttccc gctgagtgaa gtagaactcc acaaggtctt cgtgatagtt
    29221 ccacgcctga tccgtttcca gcaattttgc gtggttggct gcgccgcgtt cggtccagag
    29281 gatgagggat cgaacattgc tggcaatttt cacagagtag ctttgagata ctctgtgctt
    29341 aagttcacgt agttcatcgc cttccagcaa aaaataatgc tttccaacaa cgaatcgttc
    29401 ttcgtttcgg gaatgattct ggcgaatacg gattggtgtt gctccataac ctgccgccag
    29461 ttgttcagtg gtcacaacgc gttgaccgcg atattcgata atttgcaaat cacgggctgc
    29521 caccggcaca agttcagttg attttttcat cattacacct cacgaaaagc tgattagtgg
    29581 aacactctgg tttccggttc tgagttgtca gtggcttcac tgataagccc atccagaatc
    29641 atgtgcatcg ctctgtaacc ttccattgtg atgccggttt tttcgccgtc gcccagagcg
    29701 ccggtgaatg ctttgtacat aagaatgctt ttgagcagcc ctgattccgc tccgtaagtt
    29761 tctatgagtg cccattccat atagttctgc atagcgagac gtaatggttt caggtagatt
    29821 gtcatgccac cgttgtggcc tctgtacatg gtgcccttgt ctgtaccgcc attctcgtta
    29881 agcacttcgg tccagccgtt tctggtgagc tgttcagaga agaacatggc ggtataaagc
    29941 cagcgccagt acatgattgt cttttccggc gcgagctcac ctgtcatgcc tttttcttct
    30001 gcttcactga tgcaacacac tatttccaga ccatgccaga tcggctcgtc aaaacagcct
    30061 tcatcgagca tttccagtgc tttgttgata tcaagcagtc cgtaaggtgt gtggatacct
    30121 tcagcggtaa gaaaaaataa ctgttcagtt gcagtcatgg tttgctctct tcttaaatgg
    30181 tctttgcgcg acaagccggc aatactgatc aatccaggcg ttgtattttt ccatccaggc
    30241 atcaaactgt tttctgcgtg caaggatgcg caacaggcga tgaaaacaac gcccgtgagc
    30301 tctgtagtat tcgggcgtaa tttctcccgg atgaaagact tctccatatt ccggatgcat
    30361 caggcactgg tcaggcagac gtttttcaaa acctgaactt tcgaatacct tcgttgtcat
    30421 aaaatgagcg agatgtatga tggccgtttt tcgcgtgaag catttcttaa cttgtccgtg
    30481 gcggcagact ctgtaaacag ggccgcaatc gagttcgtat ttatgaaaag cacggtcgat
    30541 tccggcatta tttattgcac tgtattccgc atcactgaag aaaggtggaa atacgatgta
    30601 tccttccatt gtcagcgcca gaacgcgcgg atcggtttct gtcagccatt cgttttctgt
    30661 gaattcttca gaattaattt caatgatttc aggttcttct ttcatggtta ctccgtgttc
    30721 atccggtgtt aattactttt attcactctg agcgcagcca gaagatgtgg ctcagcaatt
    30781 cgtttgttgc gatatatctg tgatacagcc tggccttttc aggttgtaaa aatccccgac
    30841 aataaagctg taaattaatt actgttctta aataatgtta ttcgttttca gtccggcaca
    30901 gatgcttttc tgctttgtct gctgtacagg ataatgagcg gaaaagacaa ttcatcatgt
    30961 ttagcgcatc tggtgaacca atatcaccgc tgtcaataat cacctgtaac aaagacaggc
    31021 tttctgttat ctcgcagtgg acattatcga gtacttcaac gcaattattc atttttcatt
    31081 atctccgtta ttccggggga ggagctatgc tgttaatgat taacgcatct ggtaatatgc
    31141 ccctctttaa tgtaatcgat gcattcattc aaaagttcat caatgattaa ctttcctgat
    31201 tcagtcagat actcaccatg ttggttgata ccgacagcat cctggtatgc agtgcgtatt
    31261 gctgtttcac catctttctg cccaaactca ccacgggtaa cgccttcaaa tcgtaatagc
    31321 aactggctta tgaactgttc tgttatttca atagttgtta actctccatc atgacgcttt
    31381 acagtgagga gattacttcc tgtttttcgt ttcatccgcc gcagtgctgc aaccgttatg
    31441 cggcgacgat atgcagaaat gtattcatct ttcatttgtt atttcccgta tgcctttttg
    31501 agaaacaata ctgcaactga ccagtatccg gcatcagcca ttaataaagc ggttttatag
    31561 gcatgtttgt ctttcatgtg tcatccccat tttaaaatag tgagaactcc cgaccgcaga
    31621 ccgtcatgtt tttatgtatg attgtctgtt taatttcctt tattcgttct cagagcatca
    31681 acatactcgt aagccttttc acaggtttta ttcatggaac gaatcataca aagtaagaaa
    31741 tcatctgtct ctccgttatc accagagttt ttaaatataa actcaagcat tgacgtgttt
    31801 tcttttattt ctgctgccac ttcctcaagc ataatcaggg gagttttcat gttctttgtt
    31861 ccttaaatgc atcgcatgcg cttctggcgt attgttgtgc cagtaaaaat atgtcatccg
    31921 aaagttcatc acattcttca tcaccggaag ccgaaatgat taaccccgct tcaagcagta
    31981 ctgcaatgtg atgaaaagct gtttccggtt cgttggtgag gcctttgaac attttcatct
    32041 tacgattcct ttgtcgtgtc agaatggaaa cttgtaagta accacatcgg atcgcagtca
    32101 agtatatttg ccagaggaat cacctggctt gccagaggtt cagccttgcc gttctcccac
    32161 tggatgatgg tttcttcatc aactccaagc agcgtggcga gttcagcagt ggatagaccg
    32221 cagctttcgc gttgggtgcg gatgccatca cggcaatctt ttttgtttct cggaggtaac
    32281 agaatatgtt ttagttgttg gattttttcg attccaacct caaggagtac gtgacggttt
    32341 ctttccgata cagactccag actttcatga agcagcgatt ctacttgttc gataagcatc
    32401 aattgtgaag tagacataaa aaaacctcgt aaccaatatt tcgaaatcta ggttacgagt
    32461 gagtaatgta ggtgtcaaga gcaattaatg ttttttgttg aattaaattt gcatacgcat
    32521 ctcaacgaca acaccaataa tttcacaatc cccattgatg gggatcagtg gatagcgtgg
    32581 gtttagtggc tttaggaaac attgcccagc gtcttctatg tactgtttaa gggttgcctc
    32641 tccggagttt ttgagcctgg cgacgacata ttttcctgaa aaaacttctt tgtcagggtt
    32701 aacaagaatg ttcatgcctt caaaaataga gaactctcca ttctgtgatg tcattgagtc
    32761 ccctttcact tccagccaga agccgtgatc gcccgcatat atatcgctac caatccattc
    32821 ttctggataa gctatgtaat cttcgcagtt ccaggggcca gcactcaccc atgttagttt
    32881 cggatatcta taacggtttg gagaaactgg cttaggattt cggacgtttt gaatgctatc
    32941 tgtaggtgat ggggcgctgc cgtcaagaag ccatttagga gagcatttca atgcatccgc
    33001 gattctaaat aggtttgaag atcttgtgtc ttgtgagtct cctaactcta ttttgctgat
    33061 tgttacacgc gagacaccgg tcaattttgc caggtcatct tgtgacattc ctagctgtac
    33121 tctgcgcatt aacagtctgc caccaaagct atcgtgatcc atcttctccc ccttcttgtc
    33181 gaaataatcg tttcgaaaca aatatagcaa aaaatcttga aatctaggtt tcggaaaatt
    33241 aagattaatc gaaacaaacg tgtcgaggtg gatatggatt tgtatgaaat tctaaaaaac
    33301 atgtttggat cgaatgttga aataggtcgt tattttcccc gaaggggaag ggctagaacc
    33361 ggacaggctg taggtaagtg gaaaacccga ggtgtgccgg aagacgtggc gatcctttgt
    33421 catctggatc ccaaaattcc ttatcagcat ccgtcgctca tgaatgccag tcatgaatcg
    33481 tgatgttctg ccggaggctg tatgtcccct gattacgttc agatggagat gcctgccagg
    33541 tacagccagg cagattcaga gtggattaag cggcagttac agagcctgcc ttcctcactg
    33601 agacagaagg tcgccctgaa atacgcggag gtttacgaag ttacttttga ttccgagcct
    33661 gtttcattcc gtaaggagaa cagagcgagg catgaagcca atgtacggct gcgcagattc
    33721 gttgaaacac acgggcgtgc attacagggg tatacgactc agccgcccct ggcaggaacg
    33781 caacagcgta cctgaatggt gttggtctta aaggtgaccg acgggctgag accccacttc
    33841 tcaggatgca cttatgtact acca
//
LOCUS       DAZFRG010000023        33687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFRG010000023 DAZFRG010000000
VERSION     DAZFRG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.023"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..886)
                     /locus_tag="ACHKUC_000860"
     CDS             complement(<1..886)
                     /locus_tag="ACHKUC_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6617157.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLM"
     gene            complement(964..2187)
                     /locus_tag="ACHKUC_000861"
     CDS             complement(964..2187)
                     /locus_tag="ACHKUC_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6617158.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFTVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2213..2602)
                     /locus_tag="ACHKUC_000862"
     CDS             complement(2213..2602)
                     /locus_tag="ACHKUC_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFO6617159.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2619..3575)
                     /gene="arcC"
                     /locus_tag="ACHKUC_000863"
     CDS             complement(2619..3575)
                     /gene="arcC"
                     /locus_tag="ACHKUC_000863"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6617160.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGSIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKMAINFGTPQQQWLDAISVAEAQTLSREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3568..4992)
                     /locus_tag="ACHKUC_000864"
     CDS             complement(3568..4992)
                     /locus_tag="ACHKUC_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6617161.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNCLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVVKENHHG"
     gene            complement(4989..6548)
                     /gene="fdrA"
                     /locus_tag="ACHKUC_000865"
     CDS             complement(4989..6548)
                     /gene="fdrA"
                     /locus_tag="ACHKUC_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6617162.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNV"
     gene            complement(6643..7503)
                     /locus_tag="ACHKUC_000866"
     CDS             complement(6643..7503)
                     /locus_tag="ACHKUC_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6617163.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGRMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQAPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7509..8177)
                     /locus_tag="ACHKUC_000867"
     CDS             complement(7509..8177)
                     /locus_tag="ACHKUC_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFO6617164.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGTMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8434..9243)
                     /locus_tag="ACHKUC_000868"
     CDS             complement(8434..9243)
                     /locus_tag="ACHKUC_000868"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO6617165.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9505..10269
                     /gene="udp"
                     /locus_tag="ACHKUC_000869"
     CDS             9505..10269
                     /gene="udp"
                     /locus_tag="ACHKUC_000869"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO6617166.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(10304..11794)
                     /locus_tag="ACHKUC_000870"
     CDS             complement(10304..11794)
                     /locus_tag="ACHKUC_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO6617167.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(11807..12313)
                     /locus_tag="ACHKUC_000871"
     CDS             complement(11807..12313)
                     /locus_tag="ACHKUC_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO6617168.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(12330..13304)
                     /locus_tag="ACHKUC_000872"
     CDS             complement(12330..13304)
                     /locus_tag="ACHKUC_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO6617169.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLSLKK"
     gene            complement(13329..13958)
                     /locus_tag="ACHKUC_000873"
     CDS             complement(13329..13958)
                     /locus_tag="ACHKUC_000873"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6617170.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(13948..14952)
                     /locus_tag="ACHKUC_000874"
     CDS             complement(13948..14952)
                     /locus_tag="ACHKUC_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6617171.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(14976..15743)
                     /locus_tag="ACHKUC_000875"
     CDS             complement(14976..15743)
                     /locus_tag="ACHKUC_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6617172.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGTFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            15960..17387
                     /gene="rmuC"
                     /locus_tag="ACHKUC_000876"
     CDS             15960..17387
                     /gene="rmuC"
                     /locus_tag="ACHKUC_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO6617173.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQKIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17482..18237
                     /gene="ubiE"
                     /locus_tag="ACHKUC_000877"
     CDS             17482..18237
                     /gene="ubiE"
                     /locus_tag="ACHKUC_000877"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO6617174.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18251..18856
                     /gene="ubiJ"
                     /locus_tag="ACHKUC_000878"
     CDS             18251..18856
                     /gene="ubiJ"
                     /locus_tag="ACHKUC_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO6617175.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQCYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18853..20493
                     /gene="ubiB"
                     /locus_tag="ACHKUC_000879"
     CDS             18853..20493
                     /gene="ubiB"
                     /locus_tag="ACHKUC_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO6617176.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20572..20841
                     /gene="tatA"
                     /locus_tag="ACHKUC_000880"
     CDS             20572..20841
                     /gene="tatA"
                     /locus_tag="ACHKUC_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO6617177.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20845..21360
                     /gene="tatB"
                     /locus_tag="ACHKUC_000881"
     CDS             20845..21360
                     /gene="tatB"
                     /locus_tag="ACHKUC_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO6617178.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21363..22139
                     /gene="tatC"
                     /locus_tag="ACHKUC_000882"
     CDS             21363..22139
                     /gene="tatC"
                     /locus_tag="ACHKUC_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO6617179.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22181..22963
                     /gene="tatD"
                     /locus_tag="ACHKUC_000883"
     CDS             22181..22963
                     /gene="tatD"
                     /locus_tag="ACHKUC_000883"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO6617180.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22960..23448)
                     /gene="rfaH"
                     /locus_tag="ACHKUC_000884"
     CDS             complement(22960..23448)
                     /gene="rfaH"
                     /locus_tag="ACHKUC_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO6617181.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23615..25108
                     /gene="ubiD"
                     /locus_tag="ACHKUC_000885"
     CDS             23615..25108
                     /gene="ubiD"
                     /locus_tag="ACHKUC_000885"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO6617182.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRVTLPVDPHLEITEIADRTLR
                     AGGPALLFEKPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWSEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25154..25855
                     /gene="fre"
                     /locus_tag="ACHKUC_000886"
     CDS             25154..25855
                     /gene="fre"
                     /locus_tag="ACHKUC_000886"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO6617183.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26127..27290)
                     /gene="fadA"
                     /locus_tag="ACHKUC_000887"
     CDS             complement(26127..27290)
                     /gene="fadA"
                     /locus_tag="ACHKUC_000887"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO6617184.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTSGHDADGVLKQFNYDEVIRPETTVEA
                     LTTLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27300..29489)
                     /gene="fadB"
                     /locus_tag="ACHKUC_000888"
     CDS             complement(27300..29489)
                     /gene="fadB"
                     /locus_tag="ACHKUC_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO6617185.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAAMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEQYYPPVEPARPVGDLKTA"
     gene            29679..31010
                     /gene="pepQ"
                     /locus_tag="ACHKUC_000889"
     CDS             29679..31010
                     /gene="pepQ"
                     /locus_tag="ACHKUC_000889"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO6617186.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIANMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31010..31624
                     /locus_tag="ACHKUC_000890"
     CDS             31010..31624
                     /locus_tag="ACHKUC_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO6617187.1"
                     /translation="MESWLIPAAPVMVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31663..33114
                     /gene="trkH"
                     /locus_tag="ACHKUC_000891"
     CDS             31663..33114
                     /gene="trkH"
                     /locus_tag="ACHKUC_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO6617188.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33126..33671
                     /gene="hemG"
                     /locus_tag="ACHKUC_000892"
     CDS             33126..33671
                     /gene="hemG"
                     /locus_tag="ACHKUC_000892"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO6617189.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7981 a         8631 c         8979 g         8096 t
ORIGIN      
        1 tcattaattg tgggtttctt aaatattcta tgcaccactg acaggctttg cccataccat
       61 cggaacgagc ggcaagaaat aacttggtgg cgtgtttatg ttcgcgcatt ggctttttaa
      121 gcaatttacc gctgttcaat aacggcaatg ccagatgatg tggaatataa ccaataccga
      181 cgttttgttc tattaaggcg attgccgtag cgaagtcagg aacaaaaatg ggtttttgtc
      241 cttccaacaa ccaggcttgc atcgggggga agttaacgga ggtatcgcgg atacaaaccg
      301 ctggataatg acgcaattca ctgttttcta atggatgccg cttcgctgcc agtgggtgga
      361 gtgggctgac gacaaaatcc cattccattt gtccgaccgg ttcgctaatg atgccttcac
      421 tgcttggtac tgcatgagga gcaccgtaga ccagatcagc acgcttactg tacagcgcat
      481 cccagcagcc gttgtatacc tcaagatcta ccgtcagtgt tgttgatgtg aattgccgtt
      541 caaaatctcg aataaatgcc accagcaaat caccaggaat aatattgttt accgcaattg
      601 tcagttcacg ctcaatacca tcgtgaatca gcactgtgtt gcgctttaat gcttccaggt
      661 cgccaagaat agttttgcta tgttcgatga agtaacttcc cgctggcgat aattcaatat
      721 agcgtccctt acgaatgaac agttcaacgc ccaggctctc ttccgctttc ttgatggtgt
      781 aactgatggc ggaagggact ttatttaact gctcggctgc ggtggtaatg ctctggtgtc
      841 tggcgaccag atgaataaca cgaagcgttt cgtctgagat gaacataagg ctccctggtc
      901 agtggtaaag tggctgaatc tctccagatt cagccgtaat cattgcctga gtatacccag
      961 gaattagcgg tgttgacttg cccgactgag aatcgcatac gcgatacatg ccaccagaat
     1021 cgagtctacc gaagggatcg tcgtcagggt gaataagcct tttacggtca taaagccaac
     1081 cgctgcgcct atgatccatg ctgcgaatgt cagcacttta atctgtgact cctgggctaa
     1141 cactgactct tgatagccat tacggcgata aaggaaaaag tcagcgatat aaattccggc
     1201 aacgggtggc gtggcgatac ccaggaacag cagaaacgga ataaaccacg ccataatatc
     1261 catactgcct acgatggcgg aaaggatacc cagtgccacg gtaatctgcc atttgggaaa
     1321 gcgggtaagc agtgtggaaa ccaccagcgt gccctggaac atattgcccg cgttaccggt
     1381 gacgcaggcg aaaatcaaca acagcgccgc aggcagaacc gcaccgaata ccgccatcgc
     1441 acccagtaaa gatccctgac cactaatggc gctcggcgtt gcacccgccc agtagagcag
     1501 agggtaggca accagaaacg tcacgcctgc ggcaatcagc gcatgtttgc ggttatgcac
     1561 aaaactaccg aaatcaggca gtgtggcaac cagcacaata atggtgccaa caacggtgga
     1621 aacggcgaca cctgtattca tctccacaat cgccgctggt actgccactt cggtatgggt
     1681 tgccacatag aggatgtaac acagcaccag cgcgataacc ggcacggcaa cctgggcaat
     1741 ttttcccaga acggtaaagc caaatgccgt agaggcgaca aaaatgacac agccagccgc
     1801 cactaacagt gccagtggaa cttccagtcc atgttgagcc agtaagtcat gtaccgaatg
     1861 accaaacata ttggcggtaa cggcaatcca gccgaacaga cttatcgcca tcaaaatgtt
     1921 aatggcgatg gccccttttt cgccaaacga atatttcaca atgccatacg ttggcaggcg
     1981 ggctttttct cccacgctaa tggtgatggc tgaaagtaag gtcagaatga cgccgcctaa
     2041 caatacgacg ctaattaaac tgctgctgga aagttgattg ccgaggcttg aggatgacaa
     2101 cagtacaggt gtgacggcaa tacccagcaa aatcatggca atacgcaatc ctgatgccgt
     2161 attcagattc tcttcttttt tacgcatagt atgttccctt tataaatgcg cgttacacag
     2221 tatgaaaatg cccggtgtat ttgcgccagc tacccagttc tgtaatttcc cgttggcctt
     2281 cggtcagcca gttttctgcc agtacgccca gcacttcgct accatcggca agtttgattt
     2341 tgccaattgc cagcccagcg ggttcgctca ttaacagagc ggcaaaactg gccagcggta
     2401 attgccaaat ttctacgtcg acgtgtgttc ctccttcatt cacgcgaatc attcccggat
     2461 ggcgatcatt aatactccac aaccggtaat gggcgtcagt tcggtcttca cggacaaaaa
     2521 taccgcccgc tttttgcata tttgggttga gttccaggcc gcgcatcagc gttccgttaa
     2581 cggctaaaag cgttgtagtc attgtgttct ccagagaatt aaagggttat ccatgtaccg
     2641 ctctgatgcg ccagggctgc ttttatggtt tgcggtgaag taatcaggcc gctggcctgt
     2701 ttgccttgtt gctggctggc attgatgaaa tcaacaatgg cttccacttt gggttgcata
     2761 ctgccgacac caaactgacc ttcccgcgac agcgtttgcg cttcggcaac gctgatagcg
     2821 tcgagccact gttgttgtgg tgtaccaaag ttaatcgcca ttttttctac accggtgggg
     2881 atcaccagca gatctgcgcc taactgactg gcgagcagcg cggaggccag atctttatcg
     2941 atcacggctt ctacaccttt cagttgttgc ccgtctcgca ccaccggaat actgccgccg
     3001 ccgcaggcaa taaccagata tccctggcgc accaggtgag cgatggtgtc gtgctcaata
     3061 atttccagtg gtgcaggaga gggaactgta cgccgccaac cacgcccggc gtcctccatc
     3121 agcgtccagc cgagttgttg ttggcgttgt tgggccgttg cttcatcgag aaacgcgcca
     3181 attggtttgc tggggctggc gaaagcatcg tcatgtgggc tgactcgcgt ttgtgtcacc
     3241 agggcaatta ccggtttgtt tatgccacgg cgagccaatt cgttatgcag cgctttctgg
     3301 aacatgtagc caattgcacc ttgtgtatca cccacagcgt aatcaagtgg aaccggagaa
     3361 acttcgttac tggcgagttc agagcggcgc agaataaagc ccacctgcgg gccattaccg
     3421 tgggttagca ccaggtccca tccggcttca accatatcaa cgatatgttg cacgctttcc
     3481 atcactgcaa catattgatc gggaatacta ttgcgttgct cgtcctgaat gagcgcattg
     3541 ccgccaaccg cgacgacagc cagtggctta accatgatgg ttctccttta cgacgatctc
     3601 cgccagtgca cgtacagcgg cgacaaaaca ggccatcggc gcagtggtga tcccggcacc
     3661 aatttgcccg acccccgctt gtttgtgggc gatacccgta ttgatgaccg gtaaaatgcc
     3721 gcgatcggtc actttacgcg catcaatccc tgctgcggtg ggggcaaaat tgagggctgg
     3781 aagtgtaaat gcggggttac cgccgagggt gatggcttgc atccgacggc tgttattggt
     3841 ggcatcggca ggggtgcctc ccacaaattt gacgatcgcc ggagacgacg ccatcgcgaa
     3901 gccacccaca cctgcggttt cggtaattgc gctatcaccc aggtcagcag cagcgtcgtc
     3961 aacgccaaat ccggggaaga acagaccttc aacggcattg gcaggagcct ggaaccagcg
     4021 atcgccagta ccggaaagcc gaataccaaa attcaccccg ttacgcgcca tcaccgtaac
     4081 cattgaactg aacggtacat tggcggcggc atccatcatt gctttacagg cagccatcga
     4141 cagattgagg aagaagtgat cgttaccggt gataaatgcc acgacgcgct ggatatgttc
     4201 ctgtggaaga gagcaggcca gcaatgccgg gagcaggcgc ttaatcagca aaccggtagc
     4261 ggcagcattg cggttatgaa cttcatcccc catatgcagc gcctgcgcca ttaatggctt
     4321 gagttccagc tcgccgtgtt gtgcgattgc tgctttcatt gccggcgcca gttcatcgcg
     4381 catccaggcc agacaattga gaacttcatc gttattggcg ccaaaacgga gaaccttacc
     4441 cagaccttca ttgaaattgc taaaagtacg atggccgttg gttttgtttt ctaccaccca
     4501 taacggcatc gacgggctga tgatgcctgc cattgggcca accgcgtgat aatgatgaca
     4561 aggggcaagg tcgatttcgc ctgcgtttat ctggttttca gcgtcttgct ggctggtggc
     4621 ccaaccttcg tacagcatgg caccaataat cgcccctttt accggaccac acatctcttc
     4681 ccaggcaatc ggcgggccag agtgcaaaat acgtttttgt tcagccattg ctggaatggc
     4741 ctctttagcg agcatgacat ctaccagcac gggctgtgct tcgagataac gtgtcatggc
     4801 gatttggttg gcattttcta ccagtggatg acgtagcaac gatgccagat ccaatccagc
     4861 gtcaatatcg ccttgtgctg gtggctgcca gttgagtgca attgcgcttc ccccggcatg
     4921 ttgaatattg tcagcaaaac tgtgtagtcc cgcgttcacg accttcaaag gctgattaaa
     4981 taaggtattc atacgtttag cccctttttc tgcaactgag tctgtgcaac ggtgctggcc
     5041 catagcgccg cctgtgcatt gctgctggcg atgatgaccc ctgcattttg tagtgcgctt
     5101 atctgctgac tgcgtatttg cggatcagct tccgttccgc aaacgtgcgc aatcagcaac
     5161 ggtgcgttat tcatgtcgat gtgtgaaagt tggtccagta attcgctggc aggcgtcgtg
     5221 gaagcgccat aaccaagtac caggtcgaat aacacgacgg cagtgtgtga gtcgttgagt
     5281 tcgttgagca gacgttgatt gcgcagagtt gggtcgatca ttggatgcgg tttgccacgg
     5341 gtgaaatcat cgtcgcccat atcgatcaac gtgtgatctt cgctttgcca gatatttgcc
     5401 agagcacgat tacccgcaac tggcgtattg gaagctgcgc tgaatccctt ttgttggcag
     5461 ataagttgcg cttcgtagca gaaggttcct ccggcgaaaa caccgcgtat cgcctggcga
     5521 tgggctggca ggcgctggca ggcgttttgt aacattgtga gatcgtcgca gggtacatcg
     5581 gtttcgacag ccggaagtgg ctgagcattc agtaacgcca cagcgatgtc ggcggcgctg
     5641 gcaagtgtgg tggcggcagt gatattggga cgtgccagat catgcggatt cgccccgagg
     5701 aaattcacca ctacgggttt tccacaagcc tcggcacgct ccagaatagt ccgggcaacg
     5761 atcggtgaag gcggttttga tataagcaca atcacgcgag tttcatcgtc ttgcgccagc
     5821 gcatctaaag cgaagagcat cgatattcca ccaatctctt cactgagatc atgtccgcct
     5881 gttcccaacg cctgagaaat acccgctccg agctggtcga ttcggcaagt cacttcctgt
     5941 agcccggtgc ctgacgcgcc aataacacca attgctccgc gtttcaccac attagcgaag
     6001 cctaacggaa tgccgttaac aatggcggtt ccacagtcag gtcccattac gatgcgctga
     6061 cgttctcggg caagcgtttt gatggatttt tcctgcccga tactgacgtt atcgctgaac
     6121 atcatcacgt tcattccgag gtttaacgct ttaattgctt cggcggcggc gtagtctccc
     6181 ggtacagaaa tgagtgccag gttggcttcg ggttcggctt ccagtgccat ttccaggctg
     6241 accttttccg gtgttttaat gccactatcc gtttcatcat cgggtttact ggtcagacgt
     6301 tgctgggcca gtgccagtgc ttcattgcaa atatcttctt cacccatgac tgcaatcact
     6361 aaatcattgg gactggcgtt gatttcgtta cccagacccg catcgcgcaa ttgttcgaga
     6421 ttattgggcg ttcccatgac gaccgaggcc tgttgaatac cgggcaactt gctgatttgc
     6481 gcggaaatct gcatcagaga aactgagtcc tgataaagat tactgaaaac tttatattta
     6541 actttcatgc tgttacgcct gtttcattcc gcccgatagc aaaattcggg caaagcatcc
     6601 ccgtgtagcc ccataaacgg gctacgggag aaaaggttga aatcaattca tggtgttcag
     6661 ggttcgaaag ccatgcagca ttcctgcaag gcaatctact cctgacgttg cgccaaattg
     6721 catgacctgt tctgcacagg atgcgattgt cattgccgat gcgctcccaa ccagttgtgc
     6781 gagtaactgg caaatcggtt ctgaaaaatg tccctgaagc gcacgctcca gataatgacg
     6841 actgatatcc gtggtgttgt gcgcatgttg atcaattgcc tgctgcaacc ggtattgatg
     6901 atcggcaagt ggtgcaggga gttgccataa ccagagtgct gccagatagc ccaacaaata
     6961 gtcatctcca tcgggcgtta agcctttacc aaagccgatc agttgtggcg cctggcgttc
     7021 cagcgcatct ggatgttcca taatcgctaa tccctggtgc gtgagactat ccggcaggtg
     7081 caatgtgctt tgaacgttat gctccagaca aaataaaagc cgttgcgatg ccatcgttgg
     7141 gtagtgctgg aaaaccaaag ggaatgcctc aagtgtcagc attctcccca caaatggcga
     7201 ctgccagagc gttgcgttat cgagcgccac acaaaatcga ggtgtccgca gcaagcctgg
     7261 ggttaactga atgggatctg aatgggcaat ctctcttcgc cagtcccaac attcaggtag
     7321 ccagactctg gcagcatcag ggagattcag atacgtttgc gcacacaaca gagtgatgaa
     7381 tgttcccgcg tctgaataca gatttaccgc atgtttaaaa gcgctatgca gacgtaaatc
     7441 ttgcgtttca ttctgcggga aaagatatcc caacgacgat gcgcaaagcg taacctgtac
     7501 catcggcgtt agccttccag acctgcaata atggctttgg aatcagtaac ccaaccgaaa
     7561 atggcacctt gtgctttgat catctcgagg gcgtatttct ggaattccgg gaaataggag
     7621 ccgacacaat cttccgggat aatgcattca tagccacgat catttgcttc gcgcacggtg
     7681 gtggttacgc aaacttcggt tgttacacca caaactatca gcgtcttgat accgtggttt
     7741 tgcaaaatca gatgtaagtc ggtttgataa aacgcgcctt tgccaggctt atcaatcacc
     7801 ggttcgcctg ccaccgggta cagttcagga atgatgtcat gacctgcttc acctctgacc
     7861 agaatgcgtc ccatcggtcc tggttcacca ataaaggttt ttccgccgcg cgtcagtttg
     7921 gctggcgggc agtcgcttaa gtctgcacga tggccttcac gagtatgaat aaccatagtt
     7981 cctttttgac gtgcggctgc cagcacctct gtacatggtg cgatagccgt gcgtaccagt
     8041 gaaacatcat tgcccagagc ctcaccgaag ccccctgctt cgacaaagtc acgctgcatg
     8101 tcgatcatga ccagtgcagt tgtgcgcgga tcaaaaggta attcaaaggg ttgagcctga
     8161 aaaatggatt gcgtcatggt aaatctcctg tctgatgttt aaacgatcca gttgttctga
     8221 gaacgagtat tcaggagaaa agcgacacaa aacaggaaaa aagtttgccg cacagcaacg
     8281 aaatttttgc attcagcagg tggtgtgatg acgaatgtga agggagtcaa agagtggcat
     8341 acagcaacaa attttttgat gctgtatggt tttgagatta aaaaaatcgt tgaaattgtg
     8401 tgagcttaat catcagattt agcgactatg gcgttacttt ttaacaccat actgtgtaaa
     8461 ccattccaac attcgctgcc agccgtcttt cgcagattcg gcatgatagc tcgggcgata
     8521 atcggcgttg aacgcatgcc ctgcatccgg gtacacgata atctctgctt tcgcattagc
     8581 agcccgcagc gcctggcgca tggtttcaac gctctcctgc ggaatgctgt tatcctgacc
     8641 accatataag ccgagaaccg gagcgttaag atcggttgcg atatcaacag gttgtttcgg
     8701 tgaattcagc gacttatcgc ccgtcagttt gccgtaccac gccactgcgg cttttagctg
     8761 tggattatgc gcggcataca gccaggtgat acgtccaccc cagcagaatc cggtgatcat
     8821 taaacgatga acatcgccgc cgttgcggga agcccaactg gcgacatgat cgagatcggc
     8881 cagcacctgc gagtcaggca cttttgctac cagaccgcta agcaacgtgg ggatatcggc
     8941 aaaatcattc ggatcgcctt cgcggaagta aagttcaggt gcgatagcca gatacccctc
     9001 cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac
     9061 tacaatgacc actggcagtg ggccatcgct ttgctttggt ctggcatggt aagcaggcat
     9121 gttatcccct tgtgaaggga tagaggtgaa gcccgccaca atcgcgtcgt ccggagtctg
     9181 aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc
     9241 catggtattc tccgtaccct tataaaaatg ttgcgcaatg ttaactatag tcagcatgca
     9301 acaaatcaca ttgcctgaat cggctcatct tttatgcagt cctgcagaat gaagagtgat
     9361 ttatgtgatt tgcatcactt ttggcgggta aatttatgca acgcattttc gtcatggtga
     9421 tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt ctttttgctt cttctgacta
     9481 aaccgattca cagaggagtt gtatatgtcc aagtctgatg tttttcatct cggcctcact
     9541 aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa
     9601 aagatcgccg cgctgatgga taagccggtt aagctggcat ctcaccgcga attcaccacc
     9661 tggcgtgcag agctggatgg taaacctgtt atcgtctgct ctaccggtat cggcggcccg
     9721 tctacctcta ttgctgttga agagctggca cagctgggca ttcgcacctt cctgcgtatc
     9781 ggtacaacgg gcgctattca gccgcatatt aatgtgggtg atgtcctggt taccacggcg
     9841 tctgttcgtc tggatggtgc gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct
     9901 gatttcgaat gtacgactgc gctggttgaa gctgcgaaat ccattggcgc gacgactcac
     9961 gttggcgtga cagcttcttc tgataccttc tacccaggtc aggaacgtta cgatacttac
    10021 tctggtcgtg ttgtgcgcca ctttaaaggc tcgatggaag agtggcaggc gatgggtgta
    10081 atgaactatg aaatggaatc tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgc
    10141 gcgggtatgg tggcgggcgt tatcgttaac cgcacccagc aagagatccc gaatgctgag
    10201 acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg tggaagcggc gcgtcgtctg
    10261 ctgaaataaa aaaacgctcc ccaacttcgg ggagcgatat cagttattga cgccgtaaac
    10321 gcattatttt gcggattatt ggcgtcagta gcgtaaaggc ggccagagtc agcaatgcca
    10381 gagtgatcgg tcgggtatac agaaagtcga gtgacccacc agaaagcgac agcgcacggc
    10441 gaaaattact ttccgccatt ggccccataa tcaatgccag cactacgggt gatgccggat
    10501 atcctccttt ctgcatgaaa tagcccacca cgcctgcaat cagcataatg ccaacatcaa
    10561 aaaggctatt gttcaacgca taagtcccga ccacacaaag tgcgaggatc atcggtgtga
    10621 gaatattgcc gggaattttc accacattac cgatgacttt caaagagagc agtccaagaa
    10681 ctagtagcat gaagtaacag aagatcatgc cgacaaacag cgtataaacc atatcaccgt
    10741 ggtcggtaaa catcagcggg cctggttgca ttccctgtaa cgttaacgcg cccagcatga
    10801 tggcggttac cgcatcgcca gggatcccca gcgtcagcat aggtaacaat gcgccaccgg
    10861 tgcagccatt cgcgcctgcc tcacaggagg ccacagcttt aatttcaccc ttaccaaagt
    10921 tttctggtgt cttactgaaa cgacgagttt cgttataggc aacaaaagct gcaatatccg
    10981 cccccgcacc ggggatcatg ccgataaaaa cccctaatcc tgatgagcgc aaaatggtta
    11041 atgcaatgcg gcgtagtgtt tgccagggca gtaacagact gccgatagcc accttcgcgc
    11101 cctgacgaat ttgctggttt tgctccatag agcgaaatgc ttctgaagca gcaaataaac
    11161 caatcatcac cgggataaat ggcacattca tcagctccac aaatccacct gtaaaccgtg
    11221 gaaaaccgcc cattggatca aggcctatcg ttgcgatcag caatccgcca accccggcaa
    11281 tcagcccttt aatcagtgac tcaccggaaa tactggcaat aatgctcagt ccgaaggttg
    11341 ccagggcgaa ggactccgag gcactaaatt tcagcgccca actggcaagt accggagaga
    11401 gaaacatcag cacgataatg ctgagcgttc cgccactaaa ggaagagaac gtggcggcac
    11461 tcaatgccag ccctgctttt ccctgtttcg tcagctcata accatcaata gcggtggctg
    11521 cggcggcagg cgttccggga attttgagca aaattgcggt gatagaacca ccgtagacgc
    11581 caccaaaaaa gacgccgcaa atcatcaaca agccagaaac aggctccatg ccataggtga
    11641 aaggcagcaa aatcgccaca cccatggtcg ctgtcagacc aggcaaagcg ccaatcacaa
    11701 tacccagcat caccccaaaa gtggcaaaca gcagcgcctg aggattattg agtaatgtcg
    11761 aaaaaccctg agttaaaagt tcactttcaa acattgctgg cccctgttat tcgaaaaatg
    11821 tgccgacagg caatggcacc tgtaacgctt cgacaaacag caaccagagg attgccgtta
    11881 acagcagcgc aatacccggt gtgagtaccc atcgcctgta gcccatcaac gtcataatga
    11941 cgatgaggaa aacaggagta gtaatgagat aaccgcagta gctcatcgcc acaatataaa
    12001 acgcggttgc cgcaattcct gttgctgttt tcctgaatgc cggtttttcc tgtgcgttgg
    12061 cgcaagaggc tggttttgcc tgcaaaagat cgcgtatcac cagcaagaca gcgagcacaa
    12121 tcagggcaca ggcatagaag gtagggaagg cacttgcacc gggatccgtt tcaatggcag
    12181 ttgctggaaa atcagcggca ttaatcatca ccgcgccagc aaccacacaa agtaacagcg
    12241 ccaccaccat cgttgatgaa aggcgcttgc ggtgcggtac ggcgagttga gcaacagact
    12301 gttttgtgtt catcaaccgt acctcaggtt tattttttca ggctcaggcg agtcagcaac
    12361 tcgtcaaagt acttttcctg ttcgctgatt tgggtctgga agctggcagc gtcaagccat
    12421 gcatagttga gattcaactt ttgcagtgca tcctggaatg caggctcttc agccgtctct
    12481 tttgctgcct ttgccagaac atccaccaca tcctgcggcg ttttttgcga cacaatcagg
    12541 ccgcgccagg tgccgatgga gagatcaacg cctttctctt ttaacgtcgg gacgtcaggc
    12601 atggttttca ttcgctcatc cgccatcact accagtgtct tcagcttgcc gccattcaca
    12661 tggttgataa cttctcctgg gcttacggaa accgcttcaa tgtgcccacc taacaggcct
    12721 gtaatggcag gggctgcgcc gtcatacggg acatgagaaa actttgcgcc cgttttgtct
    12781 tccagtgcag ctgccgccag atgccagata gctccggtgc ctgagttacc aatgcgtact
    12841 tttccgggat tcgctttggc gtaagtcata aattcttcat agctattcca cggcgcatcg
    12901 gcacggactg tgatagcagc cggatccgca ttcagacggg caatgggttt aaagtcgctg
    12961 gttttaaaac gcaccattcc gaggctggga agggtggtta gttcaaccgt gcctaaacca
    13021 attttgtaac cgttagggcg ggcagcggcg atttcactca ggccgatagc accgcctccg
    13081 ccaggtttat tgataacccc gatgctgacg ggtaaatggt ttttggcggc atcagcaaag
    13141 gcacgggcaa ccagatccgt accgcctccg gcagcgtagg gaacgactaa ctcgatctgt
    13201 ttggttgggt aggaggcagc ctgtacaggc gcaatgctga gggcagcgcc tgcaagaagt
    13261 accattgcag ccattttttt cggtaaggta agatgtttta tcattgcttt tatatcctta
    13321 tttatcagtt agttgctatt taaattgttc catgcgttca taaacagtac tgcattttca
    13381 tagacttgct ctggcgtcat gccggggcga tatagcgcag aaccgaggcc aaagccgttt
    13441 gcgccgtggc tcagataacg cgccatggtt tccggtgtga taccgccaac cggtaacatc
    13501 ggaacggatt gtggaataac cgcacgccag gctttggtaa cttctggcgt aatttgttca
    13561 gccgggaaca gcttgatggc attagcacca tgttcaatgg cagcaaaggc ttcggtgggc
    13621 gtggcgacgc caggcgcaca atacataccc agcgcacggg cgcgacggat caccgcagta
    13681 tcgcaatgcg gcatcacgat aatctggccc ccagcctctt taaccgcaac cacctgctct
    13741 accgaaagca cagttccggc tccgaccagg cagtccttag gcagggcgtc gcgcagtaaa
    13801 ctgatacttt gcaacgcctc cggcgagttc ataggaatct caatcaggcg gaatccggcg
    13861 gcgtacagtt tttcaccgat ggcgacaatc tcatccggtt tcacgccgcg taaaatggcg
    13921 actaacccgg ttttttgcaa aagcgtatta aatggcatgg gtacattccc tggcgttagt
    13981 ggacagtaac ccggcagcgt gggcgattcg ccataatcca tgttcagtgg cattgggaat
    14041 ggcgtgtatc tgtctgtcgg gaaaaacgtg gctgaatgct tgtgaatagc ggctgttcag
    14101 atttgcgcta ccgattaagg cgatcggtgt tgtggcgggt aaatcacgta gcaaactgga
    14161 tgccagacca cataattcat ggcctatcag taatccggaa aggtaatccc cctgcgagga
    14221 cgacgtaaga ttactggtca gcaggcgcga acgggtcgaa aacagcgtcg ataataatcc
    14281 ggtctgatga tgctcttgtg ctgctgtcag cccgtgggtg aaggcatccc agtgcggttc
    14341 atctccagga tgcatggtgg caccgagaat cgaatggcgg gataaaacat caaataactc
    14401 gcccgtcata aaggttcgga aatcggtaat ggtgttgttt tcaactactg cccatttcgc
    14461 atgtgtgccg ggtaagccta tcagcactgg cataccctga tggatcgcgt tttgcagggc
    14521 cggttccatc gagatagcac cgaagatttg tgtctcttcg ccacgcatca cttcgggcat
    14581 ttcaccttct ttaatcacgc caggaatgat gtgcagcatt gtctgctgct ggtggataac
    14641 aggcgtcaac tgcttcgcca gaccctcgag agtgaccggg caaggtaaat aagctgctga
    14701 ttgccaccct tgcgcactgc cgagcatgcc gcaagccagt accgggcatg gggtttgtgc
    14761 cagccagtcg ccgcagacac gtaaaaacgt atcgtggaaa gcgtcctcac gtgttgctgc
    14821 ctgggagggt aatttcatga tcccccaggg gaattcacgt ttttcttgtg gatttggtga
    14881 ttcgccaaaa cgccatgccc gaagcgatga cgttccccag tccagggcaa taaatgaggg
    14941 tgctcccggc attagtcgtt tcctttaatc atggtttatt tatctttgcg gacgtgctta
    15001 ccccagccca gttcagcgga tatctcttct gcacaggatt tgacgtaagg tgccagttct
    15061 tccagacgcg ccagcgacat atacgtggtg gtactggcga cagaaatagc cgccacaatt
    15121 tcatcccggg cgttgtaaac cggcgctgca acgcaacgga tagtcggctc gttctcttcc
    15181 agatcaaaag caaaaccact ggcggcataa cgacgcatgt tctgaataaa tgtgcccagt
    15241 tttgtttcat caccacaggt cttaaacagt gtgcgccact cttcttctgt tcggttgagg
    15301 atcaatgcct tgccgatgcc ggtgattgcc agtggcatgc gatggcctgg gcgtgagcgc
    15361 atttccagcc cgcgctgact gttgatcttc tccagataca gcacttcgtc gccttcaacg
    15421 atccccagat gcacggtgtc cagggtgtaa cgggcaaggc gttgcaaaac gggctgggca
    15481 atttcgtaca gcgaggtgct ttccagtgct ctggcaccaa attcgatcag tttggcaccc
    15541 agttggtagc cctgcgttgg gacatggcgc aggtagcgtt gttccaccag aacggtgact
    15601 aaacgatggg ttgtgctccg ggtcgtgccg gtgaactcgc cgatactttt taggtcatgg
    15661 cagccgttat aaacagcatt aagaaccgcc agtccacgta ataacgtttg tgcgcctgcg
    15721 gtggttgaag aggtttctgc catacgtttc tcctgatgat attgctgaag tgttccacat
    15781 aatgacgtgg ggaaaaggtg atatatccca caatataaac tccatctcat attgtgggat
    15841 aaaataatga aaattaacga tcgttttgcg tagcgcctcg caggaaactt ctttcaaact
    15901 ggacgtttgt acagcacaat tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg
    15961 tggatttctc aatcatggtt tacgcagtta ttgcattggt gggtgtggca attggctggc
    16021 tgtttgccag ttatcaacat gcgcagcaaa aagccgagca attagctgaa cgtgaagaga
    16081 tggtcgcgga gttaagcgcg gcaaaacaac aaattaccca aagcgagcac tggcgcgcag
    16141 agtgtgaatt gctcaataac gaagtgcgca gcctgcaaag tattaacacc tctctggagg
    16201 ccgatctgcg tgaagtgacc acgcggatgg aagccgcaca gcaacacgct gacgataaaa
    16261 ttcgccagat gatcaacagc gaacagcgcc tcagtgagca atttgaaaac cttgccaacc
    16321 gtatttttga acacagtaat cgtcgggttg atgagcaaaa tcgccagagt ctgaacagtc
    16381 ttttgtcgcc gttgcgtgaa caactggatg gtttccgccg tcaggtccag gacagctttg
    16441 gtaaagaagc ccaagaacgt cataccctga ctcacgaaat ccgcaacctc cagcaactca
    16501 acgcccagat ggcccaggaa gcgatcaacc tgacgcgtgc gctgaaaggc gacaataaaa
    16561 cccagggcaa ctggggcgag gtagtattga cgcgggtgct ggaggcttcc ggtctgcgtg
    16621 aagggtatga atatgaaacc caggtcagca tcgaaaatga cgcccgctcg cggatgcagc
    16681 cggatgtcat cgtgcggttg ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc
    16741 tggtcgccta tgaacgctat ttcaacgccg aagatgacta cacccgcgaa agcgcgctac
    16801 aggaacatat cgcgtcggta cgtaaccata tccgtttgct gggacgcaaa gattatcaac
    16861 agctgccggg gctgcgaact ctggattacg tgctgatgtt tattcccgtt gaacccgctt
    16921 ttttactggc gcttgaccgt cagccggagc tgatcaccga agcgttgaaa aacaatatca
    16981 tgctggttag cccgactacg ctgctggtgg cgctgcgcac tatcgccaac ctgtggcgtt
    17041 atgagcatca aagtcgtaac gcccagaaaa tcgccgatcg cgccagcaag ctgtacgaca
    17101 aaatgcgctt gtttgtcgat gacatgtccg cgatcggtca aagtctcgac aaagcgcagg
    17161 ataactatcg ccaggcaatg aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg
    17221 cagaagcgtt tcgcggttta ggggtagaaa ttaaacgcga gattaatccg gatttggctg
    17281 aacaggcggt gagccaggat gaagagtatc gacttcggtc ggttccggag cagccgaatg
    17341 atgaagctta tcaacgcgat gatgaatata atcagcagtc gcgctagccc attgggggta
    17401 gttaagccgg gtagaaatct agggcatcga cgcccaatct gttacacttc tggaacaatt
    17461 ttttgatgag caggcattga gatggtggat aagtcacaag aaacgacgca ctttggtttt
    17521 cagaccgtcg cgaaggaaca aaaagcggat atggtcgccc acgttttcca ttccgtggca
    17581 tcaaaatacg atgtcatgaa tgatttgatg tcattcggta ttcatcgttt gtggaagcga
    17641 ttcacgattg attgcagcgg cgtacgccgt gggcagaccg tgctggatct ggctggtggc
    17701 accggcgacc tgacagcgaa attctctcgc ctggtcggag aaactggcaa agtggtcctt
    17761 gctgatatca atgaatccat gctcaaaatg ggccgcgaga agctgcgtaa tatcggtgtg
    17821 attggcaacg ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc ggataacacc
    17881 tttgattgca tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga taaagcactg
    17941 cgttcaatgt atcgcgtgct gaaacccggc ggccgcctgc tggtacttga gttctcgaag
    18001 ccaattatcg agccgctgag caaagcctat gatgcatact ccttccatgt gctgccgcgt
    18061 attggctcac tggtcgcgaa cgacgccgac agctaccgtt atctggcaga atccatccgt
    18121 atgcatcccg atcaggatac cctgaaaacc atgatgcagg atgccggatt cgaaagtgtc
    18181 gactactaca atctgacggc aggggttgtg gcgctgcatc gtggttataa gttctgacag
    18241 gagaccggaa atgcctttta aacctttagt gacggcagga attgaaagtc tgctcaacac
    18301 cttcctgtat cgctcacccg cgctgaaaac ggcccgctcg cgtctgttgg gcaaagtgct
    18361 gcgcgtggag gtaaaaggct tttcgacgtc attgattctg gtgttcagcg aacgccaggt
    18421 cgatgtactg ggcgaatggg caggcgatgc tgactgcacc gttatcgctt acgccagcgt
    18481 gttgccgaaa cttcgtgatc gccagcagct tgccgcactg attcgcagtg gtgagctgga
    18541 agtgcagggt gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt
    18601 cgaccctgcg gaactgctgg ccccttatac cggtgatatc gccgctgaag gtatcagcaa
    18661 agccctgcgc ggaggcgcaa agttcctgca tcacggcatt aagcgccagc aatgttatgt
    18721 ggcggaagcc attactgaag agtggcgtat ggcacccggt ccgcttgaag tggcctggtt
    18781 tgcggaagag acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa
    18841 actggaggct aaatgacgcc aggtgaagta cggcgcctat atttcatcat tcgcactttt
    18901 ttaagctacg gacttgatga actgatcccc aaaatgcgta tcaccctgcc gctacggcta
    18961 tggcgatact cattattctg gatgccaaat cggcataaag acaaaccttt aggtgagcga
    19021 ctacgactgg ccctgcaaga actgggaccg gtatggatca agttcgggca gatgttatca
    19081 acccgccgcg atctttttcc gccgcatatt gccgatcagc tggcgttatt gcaggacaaa
    19141 gtcgctccgt ttgatggcaa gctggcgaag cagcagattg aagctgcaat gggcggcttg
    19201 ccggtagaag cgtggtttga cgattttgaa atcaagccgc tggcttctgc ttctatcgcc
    19261 caggttcata ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa agttatccgc
    19321 ccggatattt tgccggtcat taaagcggat ctgaaactta tttaccgtct ggctcgctgg
    19381 gtgccgcgtt tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac
    19441 gaaaagacct tgattgatga actgaatttg ctgcgggaat ctgccaacgc cattcagctt
    19501 cgacgcaatt ttgaagacag cccgatgctc tacatcccgg aagtttaccc tgactattgt
    19561 agtgaaggga tgatggtgat ggaacgtatt tacggcattc cggtgtctga tgttgcgacg
    19621 ctggagaaaa acggcaccaa catgaaattg ctggcggaac gcggcgtgca ggtgttcttc
    19681 actcaggtct ttcgcgacag ctttttccat gccgatatgc accctggcaa catcttcgta
    19741 agctatgaac acccggaaaa cccgaaatat atcggcattg attgcgggat tgttggctcg
    19801 ctaaacaaag aagataaacg ctatctggcg gaaaacttta tcgccttctt taatcgcgac
    19861 tatcgcaaag tggcagagct acacgtcgat tctggttggg tgccaccaga taccaacgtt
    19921 gaagagttcg aatttgccat tcgtacggtc tgtgaaccta tctttgagaa accgctggcc
    19981 gaaatttcgt ttggacatgt actgttaaat ctgtttaata cggcgcgtcg cttcaatatg
    20041 gaagtgcagc cgcaactggt gttactccag aaaaccctgc tctacgtcga aggggtagga
    20101 cgccagcttt atccgcaact cgatttatgg aaaacggcga agcctttcct ggagtcgtgg
    20161 attaaagatc aggtcggtat tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc
    20221 tgggtcgaaa aaatgccaga actgcccgaa ctggtttacg acagtttgcg ccagggcaag
    20281 tatttacagc atagtgttga taagattgcc cgcgagcttc agtcaaatca tgtacgtcag
    20341 ggacaatcgc gttattttct cggaattggc gctacgttag tattaagtgg cacattcttg
    20401 ttggtcagcc gacctgaatg ggggttgatg cccggctggt taatggcagg tggtctgatc
    20461 gcctggtttg tcggttggcg caaaacacgc tgattttttc atcgctcaag gcgggccgtg
    20521 taacgtataa tgcggctttg tttaatcatc atctaccaca gaggaacatg tatgggtggt
    20581 atcagtattt ggcagttatt gattattgcc gtcatcgttg tactgctttt tggcaccaaa
    20641 aagctcggct ccatcggttc cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg
    20701 agcgatgatg aaccaaagca ggataaaacc agccaggatg ctgattttac tgcgaaaact
    20761 atcgccgata agcaggcgga tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc
    20821 cacgataaag agcaggtgta atctgtgttt gatatcggtt ttagcgaact gctattggtg
    20881 ttcatcatcg gcctcgtcgt tctggggccg caacgactgc ctgtggcggt aaaaacggta
    20941 gcgggctgga ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga actgacccag
    21001 gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact
    21061 aacctgacgc ccgaactgaa agcgtcgatg gatgaattac gccaggctgc ggagtcgatg
    21121 aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca caccatccat
    21181 aacccggtgg tgaaagacaa tgaaactgcg catgaaggcg taacgcctgc tgctgcacaa
    21241 acgcaggcca gttcgccgga acagaagcca gaaactacgc cagagccggt ggtaaaacct
    21301 gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa
    21361 acatgtctgt agaagatact caaccgctta tcacgcatct gattgagctg cgtaagcgtc
    21421 tgctgaactg cattatctcg gtgatcgtga tattcctgtg tctggtctat ttcgccaatg
    21481 acatctatca cctggtatcc gcgccattga tcaagcagtt gccgcaaggt tcaacgatga
    21541 tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc
    21601 tgattctgtc agcgccggtg attctctatc aggtgtgggc gtttatcgcc ccagcgctgt
    21661 ataagcatga acgtcgcctg gtggtgccgc tgctggtttc cagctctctg ctgttttata
    21721 tcggcatggc gttcgcctac tttgtggtct ttccgctggc atttggcttc cttgccaata
    21781 ccgcgccgga aggggtacag gtatccaccg acattgcgag ctatttaagc ttcgttatgg
    21841 cgctgtttat ggcgtttggt gtctcctttg aagtgccggt ggcaattgtg ttgctgtgct
    21901 ggatggggat tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg
    21961 cattcgttgt cgggatgttg ctgacgccgc cggatgtctt ctcgcaaacg ctgttggcga
    22021 tccctatgta ctgcctgttt gaaatcggtg tcttcttctc acgcttttac gttggtaaag
    22081 ggcgaaaccg ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact gaagaataaa
    22141 ttcaaccgcc cgtcagggcg gttgtcatat ggagtacagg atgtttgata tcggcgttaa
    22201 tttgaccagt tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc
    22261 gggagttaat gggctactca tcaccggtac caatctgcgt gaaagccagc aggcgcaaaa
    22321 gctggcgcgt cagtattcgt cctgttggtc aacggcgggc gtacatcctc acgacagcag
    22381 ccagtggcaa gctgtgactg aagaagcgat tattgagctg gccgcgcagc cagaagtggt
    22441 ggcgattggt gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg aagagcagga
    22501 actcgctttt gttgcccagc tacgcattgc cgcagaatta aacatgccgg tatttatgca
    22561 ctgtcgcgat gcccacgagc ggtttatgac attgctggag ccgtggctgg ataaactgcc
    22621 tggtgcggtt cttcattgct ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg
    22681 tggaatttat atcggcatta ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg
    22741 ggaattgttg ccgttgattc cggcggagaa attgctgatc gaaactgatg cgccgtatct
    22801 gctccctcgc gatctcacgc caaagccatc atcccggcgc aacgagccag cccatctgcc
    22861 ccatattttg caacgtattg cgcactggcg tggagaagat gccgcatggc tggctgccac
    22921 cacggatgcc aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc ggaactcggt
    22981 gttcttcaca ctgtgcttaa tctctttatt aataagattc agcaatagca tggagcgagc
    23041 ctcgccatcg ggctcggtga aaatggcctg aaaaccttcg aatgcgcctt cagtaataat
    23101 cactttatca ccaggatacg gcgtcgatgg atcgacaata tctttcggtt tatacactga
    23161 taactgatga atcaccgccg atgggactat cgctggcgac gcgccaaagc gcacgaagtg
    23221 gctgacacca cgagtcgcgt tgatagtcgt ggtatgaatc acttccggat cgaactccac
    23281 aaacaagtag ttggggaaca atggctcact gactgcagta cgtttgccac gcacgatttt
    23341 ttccagggtg atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc
    23401 acgttgaagt tgcccgcgct tgcagtacag taaataccag gattgcataa tgactcttat
    23461 ccgtttaatc ggggcgcaag gatagcaaaa gctttacgct aagttaatta tattccccgg
    23521 tttgcgttat accgtcagag ttcacgctaa tttaacaaat ttacagcatc gcaaagatga
    23581 acgccgtata atgggcgcag attaagaggc tacaatggac gccatgaaat ataacgattt
    23641 acgcgacttc ttgacgctgc ttgaacagca gggtgagcta aaacgtgtca cgctcccggt
    23701 ggatccgcac ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc
    23761 gctgttgttc gaaaaaccta aaggctactc aatgccggtg ctgtgcaacc tgttcggtac
    23821 gccaaagcgc gtggcgatgg gcatggggca ggaggatgtt tcggcgctgc gtgaagttgg
    23881 taaattattg gcgtttctga aagagccgga gccgccaaaa ggtttccgcg acctgtttga
    23941 taaactgccg cagtttaagc aagtattgaa catgccgaca aagcgactgc gtggtgcgcc
    24001 ctgccaacaa aaaatcgtct ctggcgatga cgtcgatctc aatcgtattc ccattatgac
    24061 ctgctggtcg gaagatgccg cgccgctgat tacctggggg ctcaccgtaa cgcgcggccc
    24121 gcataaagag cggcagaatc tgggcattta tcgccagcag ctaattggta aaaacaaact
    24181 gattatgcgc tggctgtcgc atcgcggcgg cgcgttggat tatcaggagt ggtgtgcggc
    24241 gcatccgggc gaacgtttcc cggtttctgt ggcgctgggt gccgatcctg ccacgattct
    24301 cggtgcagtc acccccgttc cggatacgct ttcagagtat gcgtttgccg gattgctgcg
    24361 cggcaccaag accgaagtgg tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc
    24421 ggagattgtg ctggaagggt atatcgaaca aggcgaaact gcgccggaag ggccgtatgg
    24481 cgaccacacc ggttactata acgaagtcga tagtttcccg gtatttaccg tgacgcatat
    24541 tacccagcgt gaagatgcga tttatcattc cacctatacc gggcgtccgc cagatgaacc
    24601 tgcggtactg ggtgtagcac tgaacgaagt gttcgtgccg attctgcaaa aacagttccc
    24661 ggaaattgtc gatttttacc tgccgccgga aggctgttct tatcgtctgg cggtagtgac
    24721 gatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt
    24781 acgccagttt atgtacacta aatttgtgat cgtttgcgat gatgacgtca acgcacgcga
    24841 ctggaacgat gtgatttggg cgattaccac ccgtatggac ccggcgcggg atactgttct
    24901 ggtagaaaat acgcctattg attatctgga ttttgcctcg cctgtctccg ggctgggttc
    24961 aaaaatgggg ctggatgcca cgaataaatg gccgggggaa acccagcgtg aatggggacg
    25021 tcccatcaaa aaagatccag atgttgtcgc gcatattgac gccatttggg atgaactggc
    25081 tatttttaac aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg
    25141 acagagaaag cgcatgacaa ccttaagctg taaagtgacc tcggtagaag ctatcacgga
    25201 taccgtatat cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta
    25261 tttgatggta gtgatggatg agcgcgacaa acgtccgttc tcaatggctt cgacgccaga
    25321 tgaaaaaggg tttatcgagc tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc
    25381 agtcatggac cgcatcctca aagatcatca aatcgtggtc gatattcccc acggagaagc
    25441 gtggctgcgc gatgatgaag agcgtccgat gattttgatt gcgggcggca ccgggttctc
    25501 ttatgcccgc tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat
    25561 ttactggggc gggcgtgaag agcagcatct gtatgatctc tgcgagcttg aggcgctttc
    25621 gttgaagcat cctggtctac aagtggtgcc ggtggttgaa caaccggaag cgggctggcg
    25681 tgggcgtact ggcaccgtgt taacggcggt attgcaggat catggtacgc tggcagagca
    25741 tgatatctat attgccggac gttttgagat ggcgaaaatt gcccgtgacc tgttttgcag
    25801 tgagcgtaat gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta tctgagatat
    25861 aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttatac agtggcatta
    25921 ctctacgttg catccggcaa acagtgcccg atgcaacgct gccgcgtctt atcgggccta
    25981 caaaagttct gaaccgtggg ccggataagg cgttcacgcc gtatccggca tacagtgccc
    26041 gatgcgacgc tgccgcgtct tatcgggcct acaaaagttc tgaaccgtag accggataag
    26101 gcgttcacgc cgcatccggc aagtggttaa acccgctcaa acaccgttgc aatgccctga
    26161 cccagaccga tacacatcgt cgccagacca aactggacgt ctttgtgttc catcagattc
    26221 agcagcgtgg tgctgatacg cgcaccggaa caacccagcg gatgacccag cgcgatcgcg
    26281 ccaccgttga ggttgatctt ctcgtcaatc tgctccatta gcccgagatc tttaatacac
    26341 ggcaggatct gcgcggcaaa ggcttcgttc atctcgaata cgccgatatc gctggcagaa
    26401 agccccgctt ttttcagcgc cagcttcgag gccggaaccg gaccataacc cataatcgat
    26461 gggtcacaac caacgaccgc catcgaacgc acgcgggcgc gcggcttaag acctaattca
    26521 cgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag cgcagaagat
    26581 gtacccgccg ttaccgtacc gctgacggga tcaaacgccg gacgcagtgt ggtgagggct
    26641 tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcaa tacgccgtcg
    26701 gcatcgtgac cactggtcgg gatgatttca tttttaaatg cgcctgactg cgtggcggcc
    26761 cacgcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct gataccgtgc
    26821 atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc gacattacgg
    26881 ctaaggccgg ggtgaaaatc gacgccgtga ctcattggca catggcccat atgctccacg
    26941 ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc gtcatgcagt
    27001 gcctgcatgg atgaaccaca caagcgatta acggtaaccg ccgggacaga gtgcggcact
    27061 tcagccaaca gtgccgcgtt acgggcgata ttaaaaccct gctccagtgt ttgctgcaca
    27121 caaccccagt aaatatcgtc aagggccgcc gcttccagtg ccgggttacg cgccagcagg
    27181 ctacgcatta aatgagcgga gagatcttcc gcacgtacgt tacgaaaagc accgcccttc
    27241 gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat tgtgactcct
    27301 taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata gtactgttcg
    27361 ttatgacgtg ctttattacg cagaccttcc ggtacttcat acagcgggcc gaggtgctga
    27421 tattgctgtg ccatatcgag gtattttgcg ctgccgaggg tgtccagcca gcggaacgcg
    27481 ccgccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc ttctgccgga
    27541 gtggcgataa tgccctcttc cagacagcgg accacttcgt tgaccatcgg gatcatcatg
    27601 cgagcgataa tctcttcttc gctgaaatcg cgcttcggct ggctcacttc tgccagcagg
    27661 tcgtcaacag cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc tttataacgc
    27721 cagaaaccga ggccgttctt ctgaccaaag cggttggcat taaacagcgc gtcgatggca
    27781 tcgcgataat ctttctgcat ccgctgcggg aagcctgctg ccatgactgc ctgggcgtga
    27841 tgcgcggtgt caatgcccac aacatccagc agatatgccg gacccatcgg ccagccaaac
    27901 tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag caactggctg
    27961 aaaccggcga aataggggaa cagtacacgg ttgacgaaga agccggggca gtcgttaacc
    28021 acaatcggcg ttttgcccat tttgctcgcc caggcgacga ctttcgcgat ggtttcgtcg
    28081 gagcttttct cgccgcgaat aatttctact aacggcattc ggtggaccgg gttaaagaag
    28141 tgcatcccgc agaagttttc cggacgttcc agcgcgttgg ccagttcgct gataggaatg
    28201 gttgaagtgt tagacgccag tacggtatcc gggcgtactt tctgctcggt ttctgccagc
    28261 acggcttttt tcactttcgg gttttcaaca accgcttcta ccacaacatc cacgcggtca
    28321 aacccggcgt agtcgagcgt tgggtgaatt gtggagatca cgccagccag tttcagacca
    28381 tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt catgccgagg
    28441 gttaacgact tgtcgttgat atctttcatg acaaccggca cgcctttcca cgcagactgg
    28501 taggcgatgc cgccgcccat aatgcctgcg cccagcacag cggcctgttt cggggtttca
    28561 acatctttgg tgagtttctt cgctttacct tttacatatt gatcgttaag gaaaatgccg
    28621 accagtgcgc gggcttcgtt ggtatgcgcc agcgggacaa aacttttgtt ttccaggttt
    28681 aaggcttctt cacgaccaaa acgggccgcc gcttcaatgg tttttactgc ggtgatgggg
    28741 gccggataat gtttccccgc cgtttgcgcg accatccctt tagcgatggt gaagctcatg
    28801 gcggcttcaa tcttgctcag ttttagcggt tccagcttcg gctgacgttt tgctttccag
    28861 tcgaggtcgc cgttaatggc ctggcgtaaa atcgccatcg ccccttcaac cagtttttct
    28921 gctttgacta caccatccac cagaccgatt ttcagcgcct gatccgcgcc gacatcttta
    28981 ccggcggcaa tgatttccag cgcactgtca gcaccgagca tacgtggcat acgtacagaa
    29041 ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg cagatccggc
    29101 gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag cgcatagccg
    29161 ttgacggcag caatggtcgg caccgggaga tcttccaggc gattaaacac gctattggca
    29221 aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa aaattcggtg
    29281 atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc ttttagatct
    29341 gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtggc agtgtcgagt
    29401 ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc cagccagtca
    29461 aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa ggtgatctgg
    29521 tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa tatgcaaata aaatattaag
    29581 gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga tgtgctaata
    29641 tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg gcctcgctct
    29701 ataaaaatca tatagctacc ttacaggaac ggactcgcga tgcgctggcg cgcttcaagc
    29761 tggatgcgtt acttattcac tccggcgaac tgttcaacgt ttttctcgac gatcatccct
    29821 atccgtttaa agtgaacccg caattcaaag cgtgggtgcc agtaactcag gtgccaaact
    29881 gctggctgct ggtggatggc gtgaataagc cgaaactgtg gttctatctg ccggttgatt
    29941 actggcacaa cgtcgaaccg ctgccgaact ccttctggac tgaagatgta gaagtgatcg
    30001 cgctgccgaa agccgatggc attggtagtc tgttgcctgc tgcgcgcggc aatatcggtt
    30061 atatcggtcc ggtgccagaa cgtgcgctgc aactgggtat tgaggccagc aatatcaacc
    30121 cgaaaggggt gatcgactac ctgcattact atcgctcctt caaaaccgag tacgaactgg
    30181 cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgcgcggca gaagaagcgt
    30241 tccgttctgg catgagcgag ttcgatatca acattgccta tctgaccgcg accggtcatc
    30301 gtgataccga cgtaccttat agcaacattg tggcgcttaa cgaacacgct gcggtgttgc
    30361 attacaccaa actggaccac caggcaccgg aagagatgcg cagcttcctg ctggatgccg
    30421 gggccgaata taacggctat gcggctgatc taacccgtac ctggtcggca aaaagcgaca
    30481 acgactacgc acagctggtg aaagacgtaa atgatgagca actggcgctg atcgccaaca
    30541 tgaaagcagg cgtcagctat gtggattacc atatccagtt ccatcagcgc atcgccaaac
    30601 tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc gaaaacgatc
    30661 tcaccggacc gtttatgccg cacggtatcg gccatccgct gggcctgcag gtgcatgacg
    30721 tagccggttt tatgcaggat gatagcggta cgcacctcgc ggcaccggca aaatatccgt
    30781 acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa ccgggtatct
    30841 acttcatcga atcgcttctg gcaccgtggc gtgaagggca gttcagcaaa cacttcaact
    30901 ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac aacgtggtga
    30961 ttcacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga tggaaagctg
    31021 gttaattcct gcggcaccgg tcatggtcgt tgaagagatc aaaaagagcc gcttcattac
    31081 gctgttggcg cataccgatg gcgttgaggc ggcaaaagcg tttgttgaat cggtgcgggc
    31141 agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc cggatgattc
    31201 tcaacagctg ggtttctctg acgacgggga gccggcggga acggcaggta aaccgatgct
    31261 cgcccaacta atgggcagcg gcgtcgggga aattaccgct gtggtggtgc gctactacgg
    31321 cggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg tgaatcaggc
    31381 gctgcgccag ctaacgaccc aacgcaagac accattaacc gaatatactt tgcaatgtga
    31441 atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca aaattatcaa
    31501 cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga aagtggctga
    31561 attttccgca aagctggcgg attttagccg tggttcattg caattgttag cgattgaaga
    31621 ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt tcgcgccatt
    31681 acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat ccctgggctg
    31741 gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt ttttgtcgcc
    31801 ctcgccattg gctctatgct ttggtggccg aaccgcaaag agaagggcga actgaaatcc
    31861 cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagcgt cggtgcgctc
    31921 ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt tgaatctttc
    31981 tctggcctga ccaccacggg ggccactacg ctggtggggc tggattcgct ccctcatgcc
    32041 atcctctttt atcgccagat gctgcaatgg tttggcggta tggggatcat cgtgctggcg
    32101 gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc agaaatgccc
    32161 ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa aaccctgtgg
    32221 ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc tggaatggat
    32281 gcctttgatg ccatcggcca tagctttgcg accatcgcta ttggcggctt ctcgacacat
    32341 gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc tatcttcctg
    32401 ctgatctctg gctgtaacta cggtctgcac ttttcactgt taagtgggcg tagtctgaag
    32461 gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagttttc actggtggtt
    32521 atttgtacac tcgtactgtg gtttcataat gtctacagtt cggcgctgat gacaattaac
    32581 caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacgac tgacagcatt
    32641 gcccgctggc cgctcttttt gccggtactg ctcttatgtt cagcttttat cggcggttgt
    32701 gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt taagcagggg
    32761 aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct acagcattaa gctggggaat
    32821 cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc ctatgcattg
    32881 gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt ttctgccttt
    32941 gcctcggttg ttgcaacatt gaataacctg gggccagggc ttggcgtggt tgctgataac
    33001 tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct gtttggtcgt
    33061 ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga atgatggagt
    33121 aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc gagattgcct
    33181 cctacctggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc aatgtgcacc
    33241 gcattgaaga accacagtgg gaaaactacg accgtgtggt cattggtgct tctattcgct
    33301 atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa gcatgcgacg cggctgaatt
    33361 cgatgccgag cgccttttac tccgtaaacc tggtggcgcg taaaccggag aagcgtactc
    33421 cacagaccaa cagctacgcg cggaagtttc tgatgagttc acaatggcgt cccgatcgct
    33481 gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac cgctttatga
    33541 tcaaactgat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa gttgtctata
    33601 ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc gacaaaccga
    33661 cgctgaaata agcataaaga ataaaaa
//
LOCUS       DAZFRG010000024        31504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFRG010000024 DAZFRG010000000
VERSION     DAZFRG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.024"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..1596)
                     /gene="cadC"
                     /locus_tag="ACHKUC_000893"
     CDS             complement(58..1596)
                     /gene="cadC"
                     /locus_tag="ACHKUC_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO6617190.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1856..1967)
                     /locus_tag="ACHKUC_000894"
                     /pseudo
     CDS             complement(1856..1967)
                     /locus_tag="ACHKUC_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2213..2288)
                     /locus_tag="ACHKUC_000895"
     tRNA            complement(2213..2288)
                     /locus_tag="ACHKUC_000895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2253..2255),aa:Phe,seq:gaa)
     gene            complement(2395..2970)
                     /gene="yjdC"
                     /locus_tag="ACHKUC_000896"
     CDS             complement(2395..2970)
                     /gene="yjdC"
                     /locus_tag="ACHKUC_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6617191.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(3007..4704)
                     /gene="dsbD"
                     /locus_tag="ACHKUC_000897"
     CDS             complement(3007..4704)
                     /gene="dsbD"
                     /locus_tag="ACHKUC_000897"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO6617192.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGSGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTVQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4680..5018)
                     /gene="cutA"
                     /locus_tag="ACHKUC_000898"
     CDS             complement(4680..5018)
                     /gene="cutA"
                     /locus_tag="ACHKUC_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO6617193.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5134..6435)
                     /gene="dcuA"
                     /locus_tag="ACHKUC_000899"
     CDS             complement(5134..6435)
                     /gene="dcuA"
                     /locus_tag="ACHKUC_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO6617194.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6553..7989)
                     /gene="aspA"
                     /locus_tag="ACHKUC_000900"
     CDS             complement(6553..7989)
                     /gene="aspA"
                     /locus_tag="ACHKUC_000900"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO6617195.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8326..8802
                     /gene="fxsA"
                     /locus_tag="ACHKUC_000901"
     CDS             8326..8802
                     /gene="fxsA"
                     /locus_tag="ACHKUC_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO6617196.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8818..10074)
                     /gene="yjeH"
                     /locus_tag="ACHKUC_000902"
     CDS             complement(8818..10074)
                     /gene="yjeH"
                     /locus_tag="ACHKUC_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO6617197.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            10350..10643
                     /gene="groS"
                     /locus_tag="ACHKUC_000903"
     CDS             10350..10643
                     /gene="groS"
                     /locus_tag="ACHKUC_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO6617198.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10687..12333
                     /gene="groL"
                     /locus_tag="ACHKUC_000904"
     CDS             10687..12333
                     /gene="groL"
                     /locus_tag="ACHKUC_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO6617199.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLETVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12471..12824
                     /gene="yjeI"
                     /locus_tag="ACHKUC_000905"
     CDS             12471..12824
                     /gene="yjeI"
                     /locus_tag="ACHKUC_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO6617200.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(12874..13743)
                     /gene="yjeJ"
                     /locus_tag="ACHKUC_000906"
     CDS             complement(12874..13743)
                     /gene="yjeJ"
                     /locus_tag="ACHKUC_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO6617201.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(13978..15006)
                     /gene="epmB"
                     /locus_tag="ACHKUC_000907"
     CDS             complement(13978..15006)
                     /gene="epmB"
                     /locus_tag="ACHKUC_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6617202.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            15048..15614
                     /gene="efp"
                     /locus_tag="ACHKUC_000908"
     CDS             15048..15614
                     /gene="efp"
                     /locus_tag="ACHKUC_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6617203.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            15666..15791
                     /gene="ecnA"
                     /locus_tag="ACHKUC_000909"
     CDS             15666..15791
                     /gene="ecnA"
                     /locus_tag="ACHKUC_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6617204.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            15902..16048
                     /gene="ecnB"
                     /locus_tag="ACHKUC_000910"
     CDS             15902..16048
                     /gene="ecnB"
                     /locus_tag="ACHKUC_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6617205.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGIGEDISDGGNAISGAAT
                     KAQQ"
     gene            16224..16541
                     /gene="sugE"
                     /locus_tag="ACHKUC_000911"
     CDS             16224..16541
                     /gene="sugE"
                     /locus_tag="ACHKUC_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6617206.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(16538..17071)
                     /gene="blc"
                     /locus_tag="ACHKUC_000912"
     CDS             complement(16538..17071)
                     /gene="blc"
                     /locus_tag="ACHKUC_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6617207.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(17160..18293)
                     /gene="ampC"
                     /locus_tag="ACHKUC_000913"
     CDS             complement(17160..18293)
                     /gene="ampC"
                     /locus_tag="ACHKUC_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class C beta-lactamase
                     EC-5"
                     /protein_id="HFO6617208.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(18356..18715)
                     /gene="frdD"
                     /locus_tag="ACHKUC_000914"
     CDS             complement(18356..18715)
                     /gene="frdD"
                     /locus_tag="ACHKUC_000914"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6617209.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(18726..19121)
                     /gene="frdC"
                     /locus_tag="ACHKUC_000915"
     CDS             complement(18726..19121)
                     /gene="frdC"
                     /locus_tag="ACHKUC_000915"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6617210.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(19132..19866)
                     /gene="frdB"
                     /locus_tag="ACHKUC_000916"
     CDS             complement(19132..19866)
                     /gene="frdB"
                     /locus_tag="ACHKUC_000916"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6617211.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(19859..21667)
                     /gene="frdA"
                     /locus_tag="ACHKUC_000917"
     CDS             complement(19859..21667)
                     /gene="frdA"
                     /locus_tag="ACHKUC_000917"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6617212.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            21992..22969
                     /gene="epmA"
                     /locus_tag="ACHKUC_000918"
     CDS             21992..22969
                     /gene="epmA"
                     /locus_tag="ACHKUC_000918"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6617213.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLLEVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            23188..24690
                     /gene="yjeM"
                     /locus_tag="ACHKUC_000919"
     CDS             23188..24690
                     /gene="yjeM"
                     /locus_tag="ACHKUC_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6617214.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            24788..25102
                     /gene="yjeO"
                     /locus_tag="ACHKUC_000920"
     CDS             24788..25102
                     /gene="yjeO"
                     /locus_tag="ACHKUC_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO6617215.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(25133..28456)
                     /gene="mscM"
                     /locus_tag="ACHKUC_000921"
     CDS             complement(25133..28456)
                     /gene="mscM"
                     /locus_tag="ACHKUC_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6617216.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(28478..29446)
                     /gene="asd"
                     /locus_tag="ACHKUC_000922"
     CDS             complement(28478..29446)
                     /gene="asd"
                     /locus_tag="ACHKUC_000922"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6617217.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(29543..30595)
                     /gene="rsgA"
                     /locus_tag="ACHKUC_000923"
     CDS             complement(29543..30595)
                     /gene="rsgA"
                     /locus_tag="ACHKUC_000923"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6617218.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            30690..31235
                     /gene="orn"
                     /locus_tag="ACHKUC_000924"
     CDS             30690..31235
                     /gene="orn"
                     /locus_tag="ACHKUC_000924"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6617219.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         7676 a         7845 c         8115 g         7868 t
ORIGIN      
        1 gaacatacaa aaactcaaca acaaatattc ccgagcatga atcaacccgg gagttactta
       61 ttctgaagcg agaaatttgt cgagataagg tacaacataa ggaacagaag tctggaatat
      121 accattttca atccagtaaa gggtgtttgc ccctgggcgt aaattaaagg cggtgagata
      181 tgcatcagct gcttcccggt tcatcccctt catttcataa accttgccaa gcaacacata
      241 atttagccag gacatttcaa gatcaatgcc ggtatttatc gcctgataag actcatctgt
      301 tttacctttt accagggcac tgaccgcttt tatttgatat ataatggaca ggttgttcaa
      361 ttccggcagt gtaacaatgt tatctatttc tgtgttcagt gctgctaatt gtttttcgtc
      421 taaaggatgt tgagaatggc gcacgatatc aactaatgct ttttctgctc tcgcgtaggt
      481 aaattctggg gatgattgaa caatctcacc taataattca ctggcacgat ccaatgattt
      541 atcatcacca tgcagtaaat aatcatgtgc ctgataaaaa ttagttaata acgcaccacg
      601 atgcggcaaa atttgctgga gcatctcctg cattcgttgt ggccacggtt ggtttaacgc
      661 ttttgataaa ctctggagca aatcattttg aatcgccagc tgattaccgt tagtgatgac
      721 ataacgttta tccagcatgg ttgaaccatc tgcattgtct accaatttta ccgacataaa
      781 gcattgttga gcacggtatt ggcgctgatt aacaaacgca atagataatg ttttaccgga
      841 actgctcggt tcatcaatgt tgtagttgat tttgtcatgc accataaagg tggagaaggt
      901 gttaagtgat gtcgccacca aatcacccac gcctatcgcg taagagagct gatacggaga
      961 actccagctg ttacaactct tattaaccat attaatgtca atatcgcgtg gattgagcaa
     1021 aatgcgcgat ttactcatag gaagacgtgt ttcaagactt gaaaacgcta ccagtgcgac
     1081 acagatacct aacgacaaca ggaaaaaaaa ccatacccaa aaggtagtga atcgtttgct
     1141 tttaactggg gattgttcag gtggcgttgt ggtgttttga atgttaagac tgtgggaggg
     1201 agaatctgtg gcaggaaccg cctctggtat agggggaggc gaagatagca ttatttcctc
     1261 tccctcttct tcgctgtacc agataaccgg caccattaat ttatagccgc gctttggtac
     1321 agtagcgata tagacaggac tatcttcatc attatctttt aatgacttac gtagttctga
     1381 gatactctgc gtcacaacgt gattggtgac aatacttctc ttccagacat tatcgataag
     1441 ttcatccctg ctaagtactt cgccactgtg ttgagcaaag aaaaccagaa gatcgattaa
     1501 tctcggctca agggtaagtt gacgcccatt gcggctaatt tggtttatgg acggagtaac
     1561 aagccattcg ccaacgcgaa ctacaggttg ttgcataata gaaactcatt cgaaaaggga
     1621 atgatgcaat gataattgcc ataacctatt ttttccatct atagatgggt ttatttacat
     1681 attattggtg aatgtaggac gttattttta cccgccataa acttcttgat tacatagtat
     1741 tacgaaagga ttttactgag aaccagaagt aattttcctt accatcaaaa ttcatcatct
     1801 ttgccaaaga aaaatgttca gaaaataatc catgaaaaat tgtccggagc gcttactatt
     1861 ttaatggatt gttagtcttt gcatgagcaa acggactgat acatttctct ttgttctcat
     1921 tcagaaaatc tcatcagttg gcgttctgcc cgatgttgtg ctttatgggt gcgttacgct
     1981 cctgatgacg tcatttgacg ttcaacagca tcacggggcc gcacgacatt tcacgtcagt
     2041 cagcgctata gctcagaaac aaattttccc gaattgggat atgcccgcaa attgctggtg
     2101 atgtggggag aatctggttg agttcggtag aattgatttg gcggcaggac gcttaaatcg
     2161 tggcgtcctg aaacgaaaaa cggacctccg tggaggtccg tttatatgaa tttggtgccc
     2221 ggactcggaa tcgaaccaag gacacgggga ttttcaatcc cctgctctac cgactgagct
     2281 atccgggcaa cggggcgcat taaaccgtaa tctgcacatc tcgtcaacct aatttcagta
     2341 aaagcgattc aactgcttaa gattgcggca aatcgcttct tttctgtgtt tctgtcaggt
     2401 caaagcacca ccctggcggc agcgggcgaa gcgcaggata tcttccgcca gccgatgtgc
     2461 ggtgtcgaca tccgcctggc tacgattcac cagcattcgg cttaaacagc cttccagcac
     2521 cagttccatc tgctttgcta ccatcgccgg atcgtcaact tccagcgtgg ttaacagttc
     2581 gtgggtgaaa tcgtaggccg cgcttttttg ctgatcggcc agttgatgaa tagggtggcc
     2641 aggatcggga taaaacgtac aggcagcgat aaacagacag cccggatagc ggttgttttt
     2701 aacgcactcc gataacgcct gataacgtgc cagcaacttt tgttcggcgg tttgtgtttc
     2761 gtccagcatc agctgacgac gccagacatc tatctgttgg ctaagataac gcagcgcatc
     2821 gtagaggatt gcctctttgt ctggccagaa gcggcgtagc tcgtccagtg ggtaatccac
     2881 acgttcagca accatctcca gcgtggtgtt ggcaatccct tgtaattcta ataatttcag
     2941 ggcttctccc agtacatctt cacgttgcac gctattttcc tccgtctttc ccactgcaag
     3001 tgtcgttcac ggttggcgat cgcgcaaatg tgcgctgaag gtttcagcat ccataaagcc
     3061 cgtgacgcgt gcttgtggat gctcctggcc ttgtccgtca aaaaagagaa ttgtcggtag
     3121 gccaaggaca ttaagatgct ttaacagcgc cacatcttgt gcgtcgttgg cagtgacgtt
     3181 agcctgaagt aagaccgtgt ctgctaacgc tttttgcacc tgcgggtcgc tgaaggtgta
     3241 tttctcaaac tctttacagg cgacgcacca gtcggcatag agatctaaca tcactggttt
     3301 gcctttggct tcaacgagcg cctgatttaa ctcatctacc gttttgattt gtgtaaagtt
     3361 gagatgcgtc tgagtttgca cggtatgcgt cgcaccaaat gcccaatcct gaagtgggcg
     3421 cacgctaacc aatgccgctg ccagcaggat tatttgcacc acgcgcatcc agccgcgttt
     3481 ggcctgtagg ctggtgataa aggcccagcc aaagaatgcg acaccaagcg ccgaccacaa
     3541 gcgtaatccc catatatcac caatcactcg ctccagcagg aagaccggca gtgcgaggat
     3601 cacaaaacca aacgcggttt tgacttgttc catccacggg ccgcttttcg gcagcaagcg
     3661 gttaccaaag acggtaatta gcatcagcgg caggcccatg cccagcgcat aaagataaag
     3721 cgtgccgctg cccagccaca tgttcccgct ttgggcgata tacagcagaa tcgcgctaag
     3781 cggtgcagtg gtgcatggtg aacagatcag tccggcaatc gcccccataa taaacacacc
     3841 gccaggtgag ccgccctgtt ggcgattgct catcagcgtg agacgtgttt gcagcgaaga
     3901 ggggagttgc agagtaaaca agccaaacat tgacatcgcc agcaaggtaa agacgatggc
     3961 gaggccaatg agcacgtatg ggtgctgtag cgccgcctgg aactgtaacc ctgcggcggc
     4021 aaccaccaga cccagcgccg tgtaagtcag cgccatcccc tgcacataaa taaaggtcag
     4081 caacaatgct ctggcagtgg aaagccgctg tttaccgccc agcacgatgc cagaaatcag
     4141 tgggtacatt ggcagcacgc atggcgtaaa ggcgatacca ataccgatca acaacgccca
     4201 gagcgcggaa aagggcaatt gcgcggtggg ctgctcttgc tgcggaacag acacaggctg
     4261 tgacgcttcg ttgttggcga ccacttcgct taacggaacg gttttggttt ctggcggata
     4321 acagaaaccg gcatcagcac agccctggta ggtgacagtt aacgttgctc ccgcactcgc
     4381 ctggttgatg gttacgggaa gcgtcagccg atcgcggtaa atctcgcttt tgccgtaaaa
     4441 ctcatcttca tgccagacgc cttgcggcag ctgcacgtcg gcaattttcg cgtgttccgg
     4501 cgtaatgcgg atctgtttac ggtagaggta gtaaccgtct ttgatctgcc aggtcagatt
     4561 caggtcatgt tggttttgct gaaaatcaaa agcaaaggct tgatccgcgg ggacaaattg
     4621 tgaacgtccc ggcgcgtcga ataatccggc aaaaacggaa gtgctgcaaa gtagcaggat
     4681 cagcgtaaag atgcgttgag ccatgagagg taatctgtgt ctccgtgtgt aacaggtaaa
     4741 accagaagtt ccggggtttg atatggatga tgagacttca ggcattccag cagtgcctgc
     4801 tggtgagata cggtagtttt taaaatcatc tgcacttcgt attcttgctc cagcttacct
     4861 tcccagtaat agagagaggt agcgccgggg atcaaggtcg cgcaggccgc cagtttttcc
     4921 gccagcactt tggcggctaa atcctgggct gtcgcttcat ccggtgccgt acatagcacc
     4981 acgacagacg tggtattcga acttttttca tcaagcataa acacctcgca agaacagatg
     5041 gaaccgcaag agagaggtca ctatacaacg gacggggaaa ggatgttagt cggcgggaaa
     5101 gaaagcgggg cgtgaacgcc ccgcgatggg tcattacagc atgaagctac ccagcacgaa
     5161 gccgaagcaa acggccaggg caacacccag agtacccggg atgaagaacg gatggttgaa
     5221 gacgaattta ccgatacggg tagtacccgt gtcatccatc tgtaccgcag caaccagcgt
     5281 cgggtaggtc ggcagaatga acagaccaga caccgcagca aaagaagcaa cagcggtcag
     5341 cggagaaacg ttcagtgcca gagccatcgg catcagtgct tttgcggttg cagcctgaga
     5401 gtacagcaga gcagaagcaa agaagaagat gacggccagc agccacggat gaccctgaat
     5461 cacttcacca gcggtatctt tgatccagtc gatgttgttg gaaacgaaag tatcgcccag
     5521 ccacgcaaca cccaggatac aaatacaggc gctcatacct gctttgaagg tgctggagtt
     5581 gagaatgttg tcggtatcga ctttacagat aacggtggtc agagttgcaa cgctgagcat
     5641 gatgatcagg attgcgttgg tggtgttcat cagtggtttt tcaaccagac ccatgcttgg
     5701 gctgttgatg attgcataga taaccacgcc aactacgccc agcaggaaca gccagacgga
     5761 cgtttttgca ccggatttga tttcaatctg cttttcaccg cgcagttcaa ccaggccctc
     5821 ttccagacgc ttgcgataaa tcggatcgtc agagagtttg gagttgaaca gcatagtgac
     5881 caggaaggac atcaccagaa ccgccagcag ggtggacggg atgaccacgg agagcagatg
     5941 gaggtagctg atgccatgac cttccatcac ggaagacatg taaaccactg ccgctgagat
     6001 tggcgatgcg gtgatcgcaa tctgcgcgga taccactgca gtagacagcg gacggcaagg
     6061 tttaacgcct tgttccttcg caacttcagc gataactggt agtgtcgcca gagagatgtt
     6121 gccagtacca gcaaagatag tcaggaaata ggtcacgatc ggtgcgagga tcgtgatgta
     6181 tttcgggtta cggcgcagca gcttttctgt ctgatgaacc agatagtcca gaccgccagc
     6241 aacctgcatg gcagaaatag cggcgataac cgccatgata atggaaatga catcgaatgg
     6301 gatgttaccg ggtttaacgc caatagcggc aagaaccagc acccccaatc cgcctgcaaa
     6361 accaatacct attcccccca atctggcgcc caagaagatc gccagcaaaa ctatgatgag
     6421 ttctacaact agcatattag ccttccttgt tttttaacaa gttgatatta aattgttatt
     6481 tttaagttac tgctcacaag aaaaaaggca cgccatctga cgtgccttct ttatttgtac
     6541 taccctgtac gattactgtt cgctttcatc agtatagcgt tttgctttgt aagccgggtg
     6601 catcagattc tgtacggaga aaatatcgtc aagttccgct tcagtcaaca gaccgcgttc
     6661 cagaacgact tcacgtacac tcttaccggt ttcggcacag attttaccca cgatgtcacc
     6721 gttgtggtga ccgatgaacg ggttcaggta agtaacgata ccgatagagt tgtaaacgta
     6781 accttcgcac acttctttgt tagcagtgat gccgttaatg catttttcca gcaggttgta
     6841 gcaagcgttg gtcagaatgt gaacggattc gaacatggcc tggccaatga ctggctccat
     6901 aacgttcaac tgcagctgac ctgcttctgc tgccatggta acagtggtgt cgttaccgat
     6961 gactttgaag cagacctggt taaccacttc cggaacaacc gggtttactt tagctggcat
     7021 gatggaagag cctgcctgca gttccggcag gttgatctcg ttcaggccgg cacgtggacc
     7081 agaagagagc aagcgcaggt cgttacagat tttggacatc ttcacagcca ggcgtttcag
     7141 cgcgccgtga accataacat aagcgccgca gtcggaggtc gcttcgatca ggtcttcagc
     7201 cggtacgcat gggaagccag tgacttcagc cagttttttc actgccagcg gagagtactc
     7261 tttcggcgtg ttcagaccag taccgattgc tgttgcgcca aggttaactt ccagcagcag
     7321 ttcagcggta cgttggatgt ttttcacttc ttctttcagc aggatgctga aggcgcggaa
     7381 ttcctgaccg agggtcatcg gtactgcgtc ctgcagctgg gtacgaccca ttttcaggat
     7441 gtcctggaat tcgacagctt tacgttcaaa gccttcacgc agttggttaa tcgcatctac
     7501 cagtttaatc agggaagagt aaactgcgat acggaaaccg gtcgggtagg cgtcgttagt
     7561 ggactgacat ttgttaacat ggtcgttcgg gttcaggtac tgatattcac ctttctggtg
     7621 acccatcagt tccagaccga tattggccag cacttcgttg gtgttcatgt ttacggaagt
     7681 acctgcgccg ccctggtaga cgtctaccgg gaactgatcc atgcattttc cgttgttcag
     7741 gacttcatca catgcggcaa tgatggcatt cgctacactt ttaggaatgg tttgcagctc
     7801 tttgtttgcc atagctgcgg cttttttaac cattaccata ccgcgaacaa attcaggaat
     7861 atcactgatt ttgttgttgc tgatatagaa gttttcaatc gctctcagag tgtgaacacc
     7921 atagtaggca tcagctggaa cttccctggt acccaacaga tcttcttcga tacgaatgtt
     7981 gtttgacatg tgaaccttct ttttcaagct gccaatgatt tgctttaaac acacagaata
     8041 tatgtggttt cgaatgtttt tcgaccgacg attatcccct gcatcgaccg aatacccgag
     8101 atcatatgct gcttgaggat ttctaccgta acctggatca ctttaagtgt cggtttttac
     8161 cccttaatta ttaatttgtg aaatggatca ccgctttggg attactacca aaaatagttg
     8221 cgcaaacatc ttgaaatttt gctaatgacc acaatataag ctaaacgcga ttcgcaaccc
     8281 attcaggtag ccgggtttaa ccggctgcta ttacaggaga aacctttgcg ctggttacct
     8341 tttattgcca ttttccttta tgtctatatt gagatttcaa tctttattca ggttgctcat
     8401 gtattggggg tattgctgac cctcgtgctg gttatattca cgtcagttat cggtatgtca
     8461 ctggtacgta accagggctt taagaatttc gtgctgatgc agcaaaaaat ggcggcgggt
     8521 gaaaacccag cggcggagat gattaaaagt gtttcgctga tcattgccgg tttgctgctt
     8581 ttattaccgg gctttttcac cgatttcctc ggtttgcttc ttttattacc gccggtgcaa
     8641 aagcatctga cagtgaagtt gatgcctcat ctgcgttttt cccgtatgcc aggtggcggt
     8701 tttagcgccg ggaccggtgg cggtaatact tttgatggtg agtaccagcg aaaggatgat
     8761 gaccgcgacc gccttgatca taaagacgat cgccaggatt aatgtcgaaa cgccggatta
     8821 tgtggttatg ccattttccg gcgtttttcg ttttggcaga aacagccata accccgccag
     8881 catgatcagc gcgtagagac ttttccagcc gaccattgcc agtaacagaa cgcataatag
     8941 cccgccaacc actgccagta gtcgataacg tccttgcaat aatttacagc ctgccagcat
     9001 gcataacaga taaatcataa taaagatgcc attggcataa ataataagag cgtccagatt
     9061 gatctctaaa gcatgaatca ccaacgtgct caccacgcag cagccgagca ccgcattgag
     9121 ggcattattc ggaatatggc gagaagagag gcgtgccagg tagtggtcag gattatgttg
     9181 cgcctgcgac cagaccaggc gggcgaagct ctgtatataa atgttgagac tggcaaagca
     9241 ggccagatag ccaattacgc aggcaatcca taacgctcct acaccgaaca actgcactac
     9301 aattttggga agcgatgctg ccgccgccat ttgttcacca taggcgtcga agtgtaagac
     9361 gactaccgta cagccccaat agactaatcc tgccagcagc aggccaatca tcaaagcacg
     9421 aggaaaatca cgctctggat ttttaaattc cgaggcaaga tgggcaaatg cttccagacc
     9481 gacaaaacac cagaacatca ctgataacgc agcaaataac ccggtaagtt cgatatttcc
     9541 tggcgcaggg aaggggatat tcgcaggttt gatatcgccc gcccaccaga tagcgacaat
     9601 cagtgcgacg ataagtccgg caataactgt ttgtagatta gcactggaac tggcacctcg
     9661 agtaccgata taccacacca gcgccagcgt accgagttct gccaacaaca gttgcccgct
     9721 atgccagcca aacattgcct ggccgaatcc ggcagcaatt tgtagcgcgg caggcaaacc
     9781 cacgggaatg accgataaaa acaaccagcc ggtgactcgc tcaagccgcg aaccaaacgc
     9841 cataccgacg aagtgtgcga cgccgcctgc gctgggatag tggcgaccca gaatcgcaaa
     9901 cacaatcgca atcgggaaca ctaagataat caaaacgggc cacgcccaca ggctgttatt
     9961 gcctgctact agcgcagcta acgcaggaac ggcaaacacg ccagtgccta ataatgacgt
    10021 cgatagcagg ccaatgccct gggccagccc cagttcttgt ttgagtccac tcatgggttg
    10081 atgtccgatt gcgcccaaat tatgggcaac tgcgtagatt ttcgatggta gcacaatcag
    10141 attcgcttat gacggtgatg aagaaattgc gatgaaatgt gaggtgaatc agggttttca
    10201 cccgattttg tgctgatcag aatttttttt ctttttcccc cttgaagggg cgaagcctca
    10261 tccccatttc tctggtcacc agccgggaaa ccacgtaagc tccggcgtca cccataacag
    10321 atacggactt tctcaaagga gagttatcaa tgaatattcg tccattgcat gatcgcgtga
    10381 tcgtcaagcg taaagaagtt gaaactaaat ctgctggcgg catcgttctg accggctctg
    10441 cagcggctaa atccacccgc ggcgaagtgc tggctgtcgg caatggccgt atccttgaaa
    10501 atggcgaagt gaagccgctg gatgtgaaag ttggcgacat cgttattttc aacgatggct
    10561 acggtgtgaa atctgagaag atcgacaatg aagaagtgtt gatcatgtcc gaaagcgaca
    10621 ttctggcaat tgttgaagcg taatccgcgc acgacactga acatacgaat ttaaggaata
    10681 aagataatgg cagctaaaga cgtaaaattc ggtaacgacg ctcgtgtgaa aatgctgcgc
    10741 ggcgtaaacg tactggcaga tgcagtgaaa gttaccctcg gtccgaaagg ccgtaacgta
    10801 gttctggata aatctttcgg tgcaccgacc atcaccaaag atggtgtttc cgttgctcgt
    10861 gaaatcgaac tggaagacaa gttcgaaaat atgggtgcgc agatggtgaa agaagttgcc
    10921 tctaaagcga acgacgctgc aggcgacggt accaccactg caaccgtact ggctcaggct
    10981 atcatcactg aaggtctgaa agctgttgct gcgggcatga acccgatgga cctgaaacgt
    11041 ggtatcgaca aagctgttac cgctgcagtt gaagaactga aagcgctgtc cgtaccgtgc
    11101 tctgactcta aagcgattgc tcaggttggt accatctccg ctaactccga cgaaaccgta
    11161 ggtaaactga tcgctgaagc gatggacaaa gtcggtaaag aaggtgttat caccgttgaa
    11221 gacggtaccg gtctgcagga cgaactggac gtggttgaag gtatgcagtt cgaccgtggc
    11281 tacctgtctc cttacttcat caacaagccg gaaactggcg cagtagaact ggaaagcccg
    11341 ttcatcctgc tggctgacaa gaaaatctcc aacatccgcg aaatgctgcc ggttctggaa
    11401 actgttgcca aagcaggtaa accgctgctg atcatcgctg aagatgttga aggcgaagcg
    11461 ctggcaactc tggttgttaa caccatgcgt ggcatcgtga aagttgctgc ggttaaagct
    11521 ccgggcttcg gcgatcgtcg taaagctatg ctgcaggata tcgcaaccct gactggcggt
    11581 accgtaatct ctgaagagat cggtatggag ctggaaaaag caaccctgga agacctgggt
    11641 caggctaaac gtgttgtgat caacaaagac accaccacca tcatcgatgg cgtgggtgaa
    11701 gaagctgcaa tccagggccg tgttgctcag atccgtcagc agattgaaga agcaacttct
    11761 gactacgatc gtgaaaaact gcaggagcgc gtagcgaaac tggcaggcgg cgttgcagtt
    11821 atcaaagttg gtgctgctac cgaagttgaa atgaaagaga aaaaagcacg cgttgaagac
    11881 gccctgcacg cgacccgtgc tgcggtagaa gaaggcgtgg ttgctggtgg tggtgttgcg
    11941 ctgatccgcg tagcgtctaa actggctgac ctgcgtggtc agaacgaaga ccagaacgtg
    12001 ggtatcaaag ttgcactgcg tgcaatggaa gctccgctgc gtcagatcgt cctgaactgc
    12061 ggcgaagaac cgtctgttgt tgctaacacc gttaaaggcg gcgacggcaa ctacggttac
    12121 aacgcagcaa ctgaagaata cggcaacatg atcgatatgg gtatcctgga tccaaccaaa
    12181 gtaacccgtt ctgctctgca gtacgcggct tctgtggctg gcctgatgat caccaccgag
    12241 tgcatggtta ccgacctgcc gaaaaacgat gcagctgact taggcgctgc tggcggtatg
    12301 ggcggcatgg gtggcatggg cggcatgatg taattgccct gcacctcgca gaaataaaca
    12361 aacccccagg cagaaatgtc tgggggtttt tcttttggtc atctttctag tataagattc
    12421 agacacggac gacgcgagtg gcgtccagct cattgattat ggggaataac atgcacgtaa
    12481 aatatttagc agggattgtc ggtgccgcgc tactgatggc gggttgtagc tccagcaacg
    12541 aattgagtgc tgccggtcag agtgtacgca ttgtggacga gcaaccaggt gcagagtgcc
    12601 agctgattgg tactgcgaca ggtaagcaaa gcaactggct ttccgggcaa cacggagaag
    12661 aaggcggttc tatgcgcggc gcagcaaacg atctgcgcaa ccaggcggct gcaatgggcg
    12721 gtaacgtgat ttatggcatc agcagcccgt cgcagggaat gttgtccagt tttgtcccga
    12781 cggatagcca gattatcggt caggtttata agtgcccgaa ctgatgtgat ccagaggctg
    12841 gatacttcca gcctcgtagt aaaacagatg agattatttc gcctgtggtg cagttttgct
    12901 ggtggattga acacgtaagt gatgcagcgc gcgtaagcat tgatcctggg ttagcggttg
    12961 ggcgttattt gccgctacgg tgcgaacata gacctgacat ggcacacccg cgagtttttt
    13021 cagtcggggg ctgaaatcaa gcattcggcg agtgatcgcc tccacttctt tacccggcgt
    13081 tgcttcacgc gttttcacca ctgcgccgct aaattgttca ttgccacttt catctttaaa
    13141 gcggtaaagc accgccagat aatggttgaa caagttatgt ttccattccg gttgggaata
    13201 ggtgtcatat tccagattgc cattttcctg gcagtcaacg tcgtataacg gcaggaaatc
    13261 taacagtgag gtaatacgta atgccagttc acgcatttcc gcattattaa ttgcatgaat
    13321 gattgctcgc gccagcattt caatctgcaa ctcattgacg actaactcac atttgctacc
    13381 gtcatgtagc gttaatacga aggtaagatt ttcgccctga tcatcggtaa gttccagcgt
    13441 gttaacccga cgattaatat cggcattttt aagctcatca accagaattt ctgggatatt
    13501 ttctgccatt ttcttaatga ctttatcgcg agcttgttca tactgaaggc gtgcagcagc
    13561 atccagttta tgcttttgat gcagacgact ttccagcgca atcagaaggt cgcgcaattc
    13621 catggcagac ataaagaaca acgattcttt attgcgaggt tctttaattt tcagcgccag
    13681 tgcaatgaaa ttattactct tgcgaataac accggtatta acacccttga tgcttatggc
    13741 catgtaggtt ctccctaacc atttctcaat taaataatta attttagttt ataagccaga
    13801 taaatgggct tggtagtaat agttgttaaa ataacataaa tagccgtgct cactctatat
    13861 aaacagaaaa gaaaggttaa ttattggtgt tggttatata aagagtaaca acagcaatgt
    13921 atatgaataa ttaaaggtta tgcaacgggc aaagattaaa cttccctgtt taatcagtta
    13981 ctgctggcgt agctggagat ccagcggcgt tttgctgggt tcgccgccaa tttctcgcgc
    14041 cagtttaggc accagatatc ccgacaccaa tgtcagcaac tcacgcataa tctgccgtgc
    14101 ttcgtcatca ctcaccataa aatgcgccgc gccctgtact ttatcgagca catgcaggta
    14161 atacggcatc acgcccgcat cgaacaacgc attactcagg tttgccagcg tttgcgcgtt
    14221 atcgttcaca ccacgtaaca gaacgctctg gttcagcaaa gtaacgccca cccggcgcaa
    14281 cttagccatc gcctgacgga atgtttcatc tacctcattg gcatggttga tgtgattcac
    14341 cagcaagatt tgcagcgtag aatgtgaaaa gcgttcaacc agcgcatctg tgatgcgtgc
    14401 cgggatcaca atcggcagac ggctgtgaat ccgcagacgt ttgatatgcg ggatggcttc
    14461 cagttgtgtg agcaaccagt ccagctcgtg atctttcgcc atcagcggat cgccgccgga
    14521 gaaaatcatc tcgtccagtt ccggatgcgc agcaacatac tcaagtgcag tctgccagtt
    14581 acgcttgttg ccctgatttt cggcataggg gaagtggcga cggaagcaat agcggcaatt
    14641 taccgcgcag ccgcctttga ccagcaaaag cgcccggttg tggtatttat gcaacaaacc
    14701 aggcactacg ctatgctgtt cttccagcgg gtcggtggag aatccgggcg cgacgacaaa
    14761 ctcatcttgc gaggtaagta cctgacgcaa aagaggatcg tccggattgc ctttctccat
    14821 gcgatcgata aatgagcggg gcacacgcag ggcaaacagc tttttggcgc tgcgtccggc
    14881 caacagtttt tcgtccgcgt ctatattcaa aagacgcaga agttcatcag gatcggtcac
    14941 aacatcggca agttgcgtta accaatcttc tctggatggg gtatttaggg ttacaatatg
    15001 cgccattttg tggcttagct accaattaac aaatttcaga gggccttatg gcaacgtact
    15061 atagcaacga ttttcgtgct ggtcttaaaa tcatgttaga cggcgaacct tacgcagttg
    15121 aagcgagtga attcgtaaaa ccgggtaaag gccaggcatt tgctcgcgtt aaactgcgtc
    15181 gtctgctgac cggtactcgc gtagaaaaaa ccttcaaatc tactgattcc gctgaaggcg
    15241 ctgatgttgt cgatatgaac ctgacttacc tgtacaacga cggtgagttc tggcacttca
    15301 tgaacaacga aactttcgag cagctgtctg ctgatgcaaa agcaattggt gacaacgcta
    15361 aatggttgct ggatcaggca gagtgtatcg taactctgtg gaatggtcag ccgatctccg
    15421 ttactccgcc gaacttcgtt gaactggaaa tcgttgatac cgatccgggc ctgaaaggtg
    15481 ataccgcagg tactggcggc aaaccggcta ccctgtctac tggcgctgtg gttaaagttc
    15541 cgctgttcgt acagatcggc gaagtcatca aagtggatac ccgctctggt gaatacgtct
    15601 ctcgcgtgaa gtaatgcggt tgtggtgcgg cctgcaggct gcaccatcac tttttcaggt
    15661 cagagatgat gaaacgcctt atcgttcttg ttttgcttgc cagcgcgctg ctcacgggct
    15721 gtaacaccgc tcgcggtttc ggcgaagata tcaaacatct cggcaactcc atctcccgcg
    15781 ctgccagcta atttttcttc tcctccgaaa aatcatcaga tttccatcat ttttggcgat
    15841 gttgtctatt attaatttgc tataggcaaa cataaataac attacctaaa aggaagacgt
    15901 tatggtgaag aagacaattg cagcgatctt ttctgttctg gtgctttcaa cagtattaac
    15961 tgcctgcaac accacgcgtg gcattggtga agacatttct gatggcggta acgcgatttc
    16021 tggcgcagca acgaaagcgc agcaataagc aataacggta cgacagctgt gtcgtgccgt
    16081 ttgttttttc tgcgatagtt acataggtaa tagttgaaat tcccctgcca cctggcaaaa
    16141 tatccgttca accatcagct ttgcaggacg acctgcaaac gcgtcttttc accggggacg
    16201 accccaattt tccggagcct gatatgtcct ggattatctt agttattgct ggtctgctgg
    16261 aagtggtatg ggccgttggc ctgaaatata ctcacggctt tagtcgtttg acgccaagtg
    16321 ttattaccgt gacggcgatg attgtcagtc tggcgctact tgcctgggcg atgaaatcgt
    16381 taccagtagg gacggcttat gccgtgtgga cgggtattgg cgcagtcggc gcggccatca
    16441 ccggcattgt gctgctcggt gagtccgcta acccgatgcg cctggcgagt ctggcgttaa
    16501 tcgtattggg gattattggt ctgaaactca gcactcatta actaccgggc tgctgtaccc
    16561 aaataaactt actgacatca aacccttccc gggtcgcgac tgccagcatc tcctgtttca
    16621 cttcgtcaga aatggttggc gtgcgggaga gtatccacag gtagtcgcgg tccgggccgc
    16681 aaaccagcgc atggcggtat tcccgatcga gtgcaataac gttataaccg ccatagaaag
    16741 gaccaaagaa tgacactttc agcgcagcgc gagttggtgc gccggtaaag tacgctttcc
    16801 cttcactctg ctgccacatt tctctgtcag ggttatagcc tttattaatg acattcaggc
    16861 cgccgtcatc acgcaggctg tatgttgcgg tgactttttc cagtccacgt tcaaagcggt
    16921 gatcaaaacg ggcaatctca taccaggtac cgagataacg tttagcgtcg aaattattca
    16981 ctacggtcac gccacgcggc ggcgtagggg aactgcaggc aacgaccaga aatgcagctg
    17041 tcgctgcggc aacgagaggg agcaggcgca taaatgtttc cttactggtt tttttctaag
    17101 tgtagatgac agcaaggaaa agcggagaaa aggtccgaaa attcggaccc gatgaaattt
    17161 tactgtagag cgttgagaat ctgccaggcg gcggcgactc tcgctggatt ggggtagttt
    17221 ttgttagcca gcatcacgat acccagctct ttttctggaa taaacgcgac atagctacca
    17281 aatccgccgg tcgcccccgt tttatgtacc catgatgcgc gtactgcagg agttgggggc
    17341 gtaatcggtt ttacggggcg tgctgccagt gcaattttat tgtcactgcc gttaatgatg
    17401 atgtcaggat ttaccggcca gtccagcatt tcccagccca gaccctgata catatcgccg
    17461 gtttgccagt agcgagattg tgccagttgt atcccttgtt gaagtgtttt ctcattgata
    17521 tcaaggggtt ttaaattgct ttgcacccag cgggccatat cttcaatggt cgacttcaca
    17581 ccataagctt cagcatctaa cgcccctggc gaaacatgta ctgccttacc ttcgcgatat
    17641 ccccaggcgt agttcttttc ttctgcggac ggtacattaa tccacgtatg attgagtttg
    17701 agtggctgga agacacgagt tttcatcgcc tgctcaaaac tcaaaccaga cggcttcaca
    17761 gccagtgcgc cgaacaaacc gatactggag ttggcataca gacgttgtgt tccaggagcc
    17821 catgcaggct gccagttttg atagaagcgc agcaagtcgc ttgaggattt cacctcatcc
    17881 ggcacctgca atggcaggcc gccagcggtg taggttgcga gatgtaatag tgtgatccca
    17941 ttccactgtt tagcggtaag ttcaggccag tattttgttg tgggatcgct taacttgatt
    18001 tcccctcgag caatagcgtc gccaccaagc acgcccgtaa atgttttgct gaccgaacct
    18061 aactcaaaca acgtttgctg tgtgacgggc tgctttttgg cgatgtccgc atagccccag
    18121 gtaaagtaat aaggtttacc ctgataaatt accgctaccg ccataccggg gatcttttgt
    18181 tgctctataa gcggggtaat tgtgcgatgc acaatatcgt tgatttgttg aggtgcggca
    18241 aatgtggagc aagaggcggt aattaataag gtgcagagcg tcgttttgaa catagggtct
    18301 ggtttccata caaaacggcc cgccataggc gggccagatt tacattggcg atgcgttaga
    18361 ttgtaacgac accaatcagt gtgacaactg tcaggatagc agccagaccg tagaaaaccc
    18421 atttacccgc aggtacgtgg attttcagat cgtgcatcgc gtggtgcata cggtgtaaac
    18481 cacaccacag cggcagaacg atcatcagga acaggaatac gcgaccaatg aagctctgcg
    18541 cgaacgccag aacgcgctcg tagctcagcg catcacccgg aaacagccct agcggcagca
    18601 gaataccaac cagcaggatc atcaccggcg caatgatggc gctccacata ccaccggccc
    18661 cgaagaggcc ccagaatacc ggttcgtcag aacgctttgg atttggatta atcatctcag
    18721 gctccttacc agtacagggc aacaaacagg attacgatgg tggcaaccac agttaccgcc
    18781 cagagacttt tgataattgg ctctggtccc attttttcgt cttttacaat gatattggct
    18841 gcttttggtg ccagctcaaa ccaggttttg gtgtgcagca gggctgccgc cagagtgatc
    18901 aggttaatga tcacgataac cgggttttgt aaaaagtcga cgaatcccgc ccaggcttcc
    18961 gggccatttt tcagggcaaa caggccgaaa atcagttcaa tgctgaacca cacagccgga
    19021 accgccgtgc cttcgcgcag catgtaaaag cgataaaacg gcaatttttt ccaccaggtg
    19081 gacgtcattg gccgtacata cggtttacgt ttagtcgtca tgttgcactc cttagcgtgg
    19141 tttcagagtc gcgataagaa agtctttcga actttctact ttgccctgct gaatggccgc
    19201 agccggatcg acgtgtttcg ggcagacttc ggagcagtag cccacgaaag tacagctcca
    19261 tacgccgttc tggctgttca actgcgccat acgctccttc ttaccgtggt cgcggctatc
    19321 ttcgttataa cgatgcgcca gcgtaatggc agccggaccg atgaactctg ggttcaggcc
    19381 aaactgcggg cacgcggcgt agcacagacc acagttgatg caaccggaga actggtgata
    19441 cttcgccatc tgcgccgggg tctggatgtt agtaccctga tccgcggtgc gggcgttgcc
    19501 gatgatgtac ggtttgatcg cttccagact ttcgatgaag tgggtcatat cgaccaccag
    19561 atcgcgttca atcgggaagt tagctaacgc ttcaaccttc ataccgtcgg tgtaatcacg
    19621 caggaaggtt ttacatgcca gttttggcac gttgttaacc atcatgccgc aggagccaca
    19681 aatcgccata cggcaggacc agcggtagct caggtccggt gccaggttgt ctttgatgta
    19741 gcccagcgca tccagtaatg aggtagttgc gtcataaggc acttcataga atgcgctatg
    19801 cggtgcggta tcgacttccg ggttatagcg caccacctca attttcaggt ttttcatctc
    19861 agccattcgc cttctccttc ttattggctg cttccgcctt atcggctgca tccgcttcgc
    19921 caccgtaaac acgtttagct ggcggcagcg tagtaatctt cacgtcgctg tactccaggc
    19981 gagtcgtgcc atcagcatcg cggaaggcga gggtgtgttt gaggaagttg acgtcgtcac
    20041 gctcggtgca accttcgtcc agacgctggt gcgcgccgcg ggactcttta cgtgccattg
    20101 cggagtgcgc catacattca gcaacgttca gaccgtggcc cagctcaatg gtgtagagca
    20161 ggtcggtgtt gaacacgctg gaagtgtcgg tgatgcgcac gcgcttgaag cgttcctgca
    20221 gctctgccag cttgtcgatg gttttctgca tcagttccgg cgtacggtag ataccgcagc
    20281 cttcttccat ggccatgccc atttcgtcgc ggatcttagc ccagttttcg ccgccatcct
    20341 ggttaaccag atctttcaga cgttgttcaa cgccagctgc ctgcgcttca attgccgctt
    20401 cgttgccatt accggcagtt gctgcacgct ctgtcgcttg ttcaccggcc agacgaccga
    20461 agaccaccag ttccgccagc gagttggagc ccagacggtt cgcaccgtgc agaccaacag
    20521 aggaacattc acccacggcg aacagacctt taatgcgggt ttcacagttc tgatcggttt
    20581 cgataccgcc catggtgtag tgtgcggtcg gacgtaccgg aatcggttct ttaaccggat
    20641 cgacgccaac gtacgctttc gccagttcgc agatgaacgg cagacgttca tgcagttttt
    20701 tctcgccgag gtgacgcagg tcgaggtaaa ccacatcgcc acgtggcgtg gagatggtgt
    20761 tgcctttacg ccattcgtgc cagaaggcct gagaaacttt gtcgcgtgga cccagttcca
    20821 tatatttgtt tttcggctcg cccagcggag tttccgggcc catgccgtaa tcttgcagat
    20881 aacggtagcc atttttgttg accagaatac caccttcacc gcggcagcct tcggtcatca
    20941 ggataccgga acctggtaga ccggttgggt gatactgaac gaattccatg tcacgcagcg
    21001 gaacgccgtg gcttagcgcc atacccatac cgtcaccggt aacgatgccg ccgttggtgt
    21061 tgtaacgata aacgcgaccc gcaccgccgg tagccataac gaccgcgtta gcacggatct
    21121 gcaccagcgt gccttccatc atgttcattg ctaccaggcc gcgaacatga ccatcatcaa
    21181 ccagaatatc cagcacgaaa tgttcgtcaa aacgctggat ctgcgggaat tgcagagagg
    21241 tctggaacag cgtgtgcagc atatggaagc cggtcttatc ggcggcgaac caggtacgct
    21301 cgattttcat gccgccgaag cgacgtacgt tgacgctacc atccggacga cggctccatg
    21361 ggcaccccca cagttccagt tgggtcattt cggttgggca gtggtggacg aaataatcca
    21421 cgacatcctg ctcacacaac cagtcgccac ccgctactgt atcgtgaaag tgatattcga
    21481 agctgtcatg atcctgcgcg acagcggcgg agcccccttc tgcagcaacg gtatggctac
    21541 gcatcgggta tacttttgag attagtgcga tttttgcatt tggatttgcc tgcgcggcag
    21601 caattgcagc acgtaatccc gcgccaccgg cgcctacaat ggcaagatcg gcttgaaagg
    21661 tttgcacgac attcctccag attgttttta tcccacagcc ccgtacttca gggtaagtgc
    21721 ctgaaagtta cggtctgcga acgctattcc actgctcctt tataggtaca acagtatagt
    21781 ctgatggata ggtctgaaat ttgacgagat cgattttttt agtgcgcaag agggctaaat
    21841 tatcactgaa gatgattaat ttaattacta aaccatcaga tcgtgctttt tttagtcact
    21901 gcccaccgtt gttttattcc tgcgtaaaat ttgtctcgcc gtcgcgttgc gagtagactt
    21961 cgtgcccttg tcaaaaactg gagatttaac tatgagcgaa acggcatcct ggcagccgag
    22021 cgcatccatt cctaacttat taaaacgcgc ggcgattatg gcggagatcc gtcgtttctt
    22081 tgccgatcgt ggagtgctgg aggtggagac gccttgtatg agccaggcga cggtaaccga
    22141 tattcatttg gtcccgtttg agacacgttt cgttggcccc gggcattcgc aggggatgaa
    22201 tctctggtta atgaccagcc cggaatacca tatgaagcgc ctgctggttg ccggttgtgg
    22261 gccggtattc cagctgtgcc gcagcttccg taatgaagag atggggcgtt atcacaaccc
    22321 tgagttcact atgcttgagt ggtatcgacc gcactatgat atgtaccggt tgatgaacga
    22381 ggtggacgat ctcttgcaac aggtgctgga ctgtccggca gcagaaagcc tttcttatca
    22441 acaagctttc ttgcgttatc tggaaattga cccactatct gccgacaaaa cgcaactgct
    22501 ggaagtggca gcgaaactgg atttgagcaa cgttgccgat actgaagaag accgcgacac
    22561 gcttctacaa ttgctgttta cctttggcgt agagccaaat attggcaaag aaaaaccgac
    22621 ctttgtgtac cactttccag ccagccaggc atcactggcg caaatcagta ccgaagatca
    22681 tcgggtcgct gaacgctttg aggtttatta taaaggtatt gagctggcga atggtttcca
    22741 tgaattgacg gatgcccgtg agcagcaaca acgctttgaa caagataacc gtaagcgcgc
    22801 ggcgcgcggt ttgccgcagc accccattga ccagaatctg attgaagcct tgaaagtcgg
    22861 tatgcctgac tgttccggcg tggcattagg tgttgatcgt ctggtgatgt tggcgctggg
    22921 cgcggagaca ctggctgaag tcatcgcctt tagcgttgac cgggcataat tctgaaaaat
    22981 tactgaatta acagcgaaga atggcgtgat aagcgccatt ctgtagcata tgttttcatt
    23041 tttccgctaa aggccacaat ttaagactgg atatttgata tcatccaggt atcaattctg
    23101 tattggtttt tacgctgccc gctcaatgtt gggcagtgca gttcagtggg cgcgttttat
    23161 acacgcgctg aaatgaagga tggtttcatg cctcactcga taaaaaagat gagtctgata
    23221 ggactcatat tgatgatctt tacttctgta tttggatttg ccaatagccc atcggcttat
    23281 tacttaatgg gttatagtgc gattcccttt tatatatttt ctgcattgtt attctttatt
    23341 ccattcgcct taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggcggtatc
    23401 tattcctgga tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg
    23461 ttttcctctt atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca
    23521 acattcctct acggtagcga catgacccag cactggcata ttgccggact ggagcctacg
    23581 caggtggttg gtctgctggc agtggcatgg atgattctgg tcaccgtcgt tgcttcaaaa
    23641 gggattaata aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat
    23701 ttagtattgc tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag
    23761 gatattaatt tccttgcatc accgaaccca ggttatcagt ccggtctggc aatgctatcg
    23821 tttgtggtat ttgccatttt tgcctatggc ggaattgaag cggttggtgg tctggttgat
    23881 aaaacggaaa atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt
    23941 tcaatcggtt attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta
    24001 ttaagtaatg gttccgttaa cctcggcaat attacctatg tgctgatgaa gagcctcggg
    24061 gtgacgctgg gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg
    24121 tttgcgcgta ttaccggact gtcgatgttc ctcgcttata ccggtgcgtt ctttacactt
    24181 tgctattcac cgctgaaagc catcatccag gggacgccga aagcattgtg gccggaaccg
    24241 atgacgcgcc tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgtgggttg
    24301 gttactgtct tcatcctgct ggtttcgttt ggtggcggta ccgcatcggc gttctttaac
    24361 aagctgacgc tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct
    24421 ttcccatttt ttaaagcacg tcaggatctc gacagaccgt ttgtgatttt caaaacgcat
    24481 atgtcggcaa tgattgcaac agtggttgtc gtactggtgg tgacatttgc gaacgtcttc
    24541 accatcattc aacctgtggt tgaagctgga gactgggaca gcacattgtg gatgattggc
    24601 ggccctgtct tcttctcgct gttagcgatg gcgatttacc agaactattg cagcagagtg
    24661 gtaaaaaatc cgcagtgggt ggctgagtaa tcaaatgccc tatcgtcggc ctgacgatag
    24721 ggcgtaatga cagggaatta gtgagatttc gtatgcgcta tagcgaaatt aaactatggg
    24781 ttacctcatg agtgcgcgca tgtctgtctt gtgctgcatc tggttcattg tggcgttcct
    24841 ctggataacc attacctccg cgctggacaa agagtggatg attgatggac gcgggattaa
    24901 caacgtctgt gatgttttga tgtatctcga agatgacgat actcgcgatg tcggtgtgat
    24961 aatgacgtta ccgctctttt tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg
    25021 ctggtttatg tacgccaccg cgctggctat tttcggctat tggctgtggc agttttttct
    25081 gcgctatcag ttttgcttat aagccggatg ggttcatccg gccacaaaga ccttacaaac
    25141 ttcccgcctg acgacctttg cccgcagaag ttaatgttct ccccgtttgc ttaccattca
    25201 ggctttccaa acgcatctgg aagggcggga atggcatatc aataccgtgg gcatggaatc
    25261 ctgccagaat tagctggtga atctcatggc gtagtggcat acggtgaccc atctcagcgg
    25321 cgtaaatacg cagctcgaaa atttgaatcc cctgttgcag atccaccagg aagacttccg
    25381 gcgccgggtt gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca
    25441 cttcttcact gttggcatcg gcaggggccg gaatcgtcaa taccacgcgc gtgaccgagt
    25501 cagagagcga ccagttgata aactgctcgg taataaacgc cttgttcggc acaattatct
    25561 ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccgg
    25621 tgagatcgcg gatcgttacc gtatcgccaa tacgaatcgg tttttcgaac aggataatca
    25681 ggccagagat aaagttggcg aaaatctcct gtaaaccaaa gcccagacca acaccgagcg
    25741 cggcgaccag ccactgcaat ttcgaccact caataccaat catcgagaag ccgaccagcc
    25801 cgccaatcag catcagcaga tatttggtga tggtggtgat ggcgtagccc gtacccggcg
    25861 ttaaatccag gtgctgcaga atcgccagtt ccagcagcgc gggcaggttg cgcaccagct
    25921 gcgtggtgat gataaacacc agaatggcaa tcagtaccgc accgagggta atcggctcca
    25981 ggctttctac gccctgtacc gtggaagtga catcccacag cgaaatattt tcgaggaagc
    26041 cgaaagcaga atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca
    26101 aaattgagcg caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt
    26161 catcgacttc aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg
    26221 cgcgctgcgc taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcggc
    26281 gctggatcag catccagcgg cggataacgt gataaaccac cagtagcaaa aaccagatgg
    26341 caaccgaggt ttcaagcctc gctaacagcg cctgcgccgt tgccagataa cccaccgccg
    26401 acgccagaat ggcaaccagc ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa
    26461 tgttgtcgcc gctgccctct ttgttgagat acagcgggat cccggccttt ttcaggctga
    26521 gggtgaccac cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg
    26581 agaattcacg gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ggcacaataa
    26641 gcccgatgct catcaggtag taacgcatcc cacgggaaac tcgttcgcgc ggccagccaa
    26701 aatgagcgat aaacaagccg ttcgggcgag caaaggtggc gcaaatcatc actacccaca
    26761 gcagcggcac ggtggctgtt acgccatcac caatcgcgac cgccagcgga taaggccacg
    26821 cctcgcgcaa gccgtaaccc agtgtcatcc acagcacagg cagcggtgac gcgacgagaa
    26881 tcgaccagaa aagagtgcgt aacgtcagcc agaagtgatc ctgagtcact ttgccgactt
    26941 tcgccgccga acgttcaaga aaacgggtga aatagcggcg cgagtaaata ctgcaaccga
    27001 ccagaatcaa cgcgccaaac agcggcaaaa tcgtctcttt actggtcagc atcatcacac
    27061 tggctttgcc caactgactg aaggtgtcca gcgaaatgag acgacgcaga tcctgggcga
    27121 tttccagcgg ccaggcgatg gtcatcgggc gcacgtcaga ggtccagaac agatagcggt
    27181 gcgtcgcttc gttcacctct ttcagcgcat cctccagttg cccgttggag actttcagct
    27241 tggtcagctc cagcagtagc gtgtcgccac cctgcaacaa tgagttcagc aactcacgct
    27301 gagtgcgtag ctgtgcttcc agaatacggt tttgctcagc ggtcagtggc tgaccgtcgg
    27361 cctgatggat ttgccgtagc agcggctgtt tattaagcag atcctcataa cgtaaccgtt
    27421 gcacacgtaa ctgcgccatt tcggtatcaa gctgttgtgg ttttggcatt tccggtagcc
    27481 gtgccacctg cgcccgcaac gcttcgccga gcaggttaga tgatcccagc cattgcgact
    27541 gttcacgcag cgtattcaac gcctgccgga cctgtaacgt ctggctggca gcctgacgct
    27601 gttgcgaggc aacgagatcc atccgctgcg cctgttgatt cagcgccgcc gatagttcgc
    27661 ggttaatttt aaactgggcg acgatatctt ttggcaaatc ggcgctactt tccgccagtt
    27721 gctcggtact ttccagcgcc cgttccgctt ccagttggcg ttgactattc aactggttgc
    27781 gtagtgcttg caaatatgca tccagctgct ggctctcttt ttccgccagc tcagagcgca
    27841 agcgcgctaa ttcctggcgg ttattggcag atagctgcgc cagctccagt tcatcaacga
    27901 gcgctttcag acgtgcggag tcagactgca acgcgaaatt ttgtgcctga ttgagcgggg
    27961 tattgccggt aagtgtcccc aggcggcgct cgatttcatt caactgacgg cgggcatcgg
    28021 tttgctgttg cggcagttga ttcagcgaat cggcaatctc gcgggcgcgc tcctgctctt
    28081 gctgggcctg acggctttta tccagcaact gactgctgat ctggagaatt tcctgattca
    28141 gcgcatcgtt agacattccc ggcgacacgc tgcgtggctc gtcacgcatg ttgtttaact
    28201 gcgcgcgcag agtggcggaa agtttcggat aattatcaat gacttcttga tattgcttga
    28261 tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa ggcagactgg agcgcctcta
    28321 cgacttccgg ctgcgcaggt ttcgccgctt ttgcctgctc cagttcctgg gtgatttgtt
    28381 tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag gcaccaggcc atcagaaaag
    28441 tgataatcag gcgcacgtca gcgtttcctt tgatggatta gacctggtct tttttgtcgt
    28501 caaccaatgg gctggcgtcg tgttctgcct cgatttcttc agcaggaagc ggggctggtt
    28561 cagcgtctgg cgtaacaaag gtttcggtag atactgccag cggctggcca attttcgtga
    28621 cagacaggct ttccagttgc tcaaccaggt tcactttacc tggtgcaaac aggttgataa
    28681 cggtagagcc aagtttaaag cgacccattt cctggccttt cagcaatgcc acagaaccgt
    28741 cgttttcccc ggcaggccag gtccagcgct tgatgatgcc ttcgcgcggc ggcgtaatgg
    28801 tgcccgccca gaccgtctca atgctgccta caatcgtcgc accgaccaga atctgcgcca
    28861 tcgggccaaa ttcggtatcg aacaggcaga tcacgcgttc gttacgggca aacagattcg
    28921 gcacgttctg agccgtgaga tggttaacgg agaagagatc gcccggcacg tagatcattt
    28981 cacgcagaat accgttgcac ggcatgtgta cgcggtggta gtcacgtggg gagaggtaag
    29041 tggtcacaaa cgtaccgtta cggaagagat ccgccatcag atagttgcct gccagcaggg
    29101 cttcgaggct gtagttgtgg cctttggctt gcaggatttt atcttcttcg attttaccca
    29161 actggctgat aacgccatcg gcaggcataa ccagtacatt cggatcggta tcgattgggc
    29221 gtacttcgtc acgcagcgga cggacaaaga attcgttaaa ggtgcggtag ctggcggtgt
    29281 ccggcttttg cgcctctttc atgtcgacct tgtagtattt aacgaacaga tcgataacca
    29341 gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc cgccaggcga gtaagccata
    29401 gtttcggcag aatgtactgt agcgaaagtt taaatgaatt taacaaggta gcctccaggc
    29461 cattgttttg tcgttcctga tccggcctac atgccggatc ctgaaaaaaa ggggacgatt
    29521 ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga aaagttttta cgcgttttta
    29581 cctgcgccat gctttccaga atacggtgat agttttcgaa acgggtttcc gcgattttcc
    29641 cttcctcaac cgcttcccgg atagcgcagc ccggatcggt atcgtgtttg caatcgcgat
    29701 atttacacag acctaaatag tcatggaatt cgacaaagcc ctgagtgatt tgttccggct
    29761 ccaggtgcca gagaccaaac tcacgcacac ctggggaatc aatcacatca ccgccgtgcg
    29821 ggaagtgata cagtcgtgcg gcggtcgtgg tgtgctgacc aagccccgag ttgtcagaga
    29881 catcgttggt caggatctct ttttgcagcc ccagcaacgc gttcagcagg ctggatttgc
    29941 caacgccaga ctgcccggca aaaatgctga tgcgcccggt caacgcctct tccagcggtt
    30001 tcagcccatc ctgagtatga ctggaaacca tcaatacgcg ataaccgata ttgcggtaga
    30061 tatccatctg ctcgttgacg aactccatgc cttcgtcgtc cagcaggtct atcttgttga
    30121 gcacaataat cggctcaatc tgcaaggttt cgcaggccac caggtaacgg tcgataatat
    30181 tgagcgacag ctccggcaaa atggcggaga caatgacaat ctggtcgatg ttggcagcaa
    30241 taggtttcac accgtcgtaa aaatccgggc gcgtcaacac tgaggtgcgc tcatgcaccg
    30301 cttcgacgat ccctttgaca tttacgcctt ccgccgctgg tttacccgga cgccagacta
    30361 cgcggtcgcc ggtcaccaac gaacggatgg tacggcgaat attgcagcgg tgaacgtcgc
    30421 catcggcgga ttccacatca gcgtgcatac caaagcggct gatgacgata ccttcatcag
    30481 gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt agacgtttta agacgacgct
    30541 ggtgattggc gttcacgcgg cgctgctggc ctttggagag tttattttta ctcaatcgta
    30601 cagactcctg gtcgcccctg atgggcaaaa catctatgat acacgcaatt gtggatcaat
    30661 atagtcactg tgaatgggtg gaaaatagca tgagtgccaa tgaaaacaac ctgatttgga
    30721 tcgatcttga gatgaccggt ctggatcccg agcgcgatcg cattattgag attgccacgc
    30781 tggtgaccga tgccaacctg aatattctgg cagaagggcc gaccattgca gtacatcagt
    30841 ccgatgaaca gctggcgctg atggatgact ggaacgtgcg cacccatacc gccagcgggc
    30901 tggtagagcg tgtgaaagcg agcacgatgg gcgatcgtga agctgaactg gcaacgctcg
    30961 aattcttaaa acagtgggtg cctgcgggaa aatcgccgat ttgtggtaac agcatcggtc
    31021 aggaccgtcg cttcctgttt aaatacatgc cggagctgga agcctacttc cactaccgct
    31081 atctcgatgt tagcaccctg aaagagctgg cgcgccgctg gaagccggaa attcttgacg
    31141 gctttaccaa gcaggggacg catcaggcga tggatgatat ccgtgaatcg gtggcggagt
    31201 tggcttacta ccgcgagcat tttatcaagc tgtaaaattt ttcctggtca cgtaagcgcc
    31261 ggaatccact tgcggcggcg tgattttgac gctaaattga acgtcttgct gataatttca
    31321 gcacttgaga taaaaactca aaaaaaattt tttagggggg ttgcagaggg aaagatttct
    31381 cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa ttacgaaagc aaaattaagt
    31441 agtacgcggg aatagctcag ttggtagagc acgaccttgc caaggtcggg gtcgcgagtt
    31501 cgag
//
LOCUS       DAZFRG010000025        31447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFRG010000025 DAZFRG010000000
VERSION     DAZFRG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.025"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHKUC_000925"
     CDS             complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHKUC_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO6617220.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHKUC_000926"
     CDS             complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHKUC_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO6617221.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETSRINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4601..5248
                     /gene="acrR"
                     /locus_tag="ACHKUC_000927"
     CDS             4601..5248
                     /gene="acrR"
                     /locus_tag="ACHKUC_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO6617222.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5376..8738
                     /gene="mscK"
                     /locus_tag="ACHKUC_000928"
     CDS             5376..8738
                     /gene="mscK"
                     /locus_tag="ACHKUC_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO6617223.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8799..8897
                     /locus_tag="ACHKUC_000929"
                     /pseudo
     CDS             8799..8897
                     /locus_tag="ACHKUC_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="ACHKUC_000930"
     CDS             complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="ACHKUC_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO6617224.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9125..9652)
                     /gene="priC"
                     /locus_tag="ACHKUC_000931"
     CDS             complement(9125..9652)
                     /gene="priC"
                     /locus_tag="ACHKUC_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO6617225.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9722..10099
                     /gene="ybaN"
                     /locus_tag="ACHKUC_000932"
     CDS             9722..10099
                     /gene="ybaN"
                     /locus_tag="ACHKUC_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO6617226.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10252..10803
                     /gene="apt"
                     /locus_tag="ACHKUC_000933"
     CDS             10252..10803
                     /gene="apt"
                     /locus_tag="ACHKUC_000933"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO6617227.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10932..12863
                     /gene="dnaX"
                     /locus_tag="ACHKUC_000934"
     CDS             10932..12863
                     /gene="dnaX"
                     /locus_tag="ACHKUC_000934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO6617228.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12196..12260
                     /gene="dnaX"
                     /locus_tag="ACHKUC_000934"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12916..13245
                     /gene="ybaB"
                     /locus_tag="ACHKUC_000935"
     CDS             12916..13245
                     /gene="ybaB"
                     /locus_tag="ACHKUC_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO6617229.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13245..13850
                     /gene="recR"
                     /locus_tag="ACHKUC_000936"
     CDS             13245..13850
                     /gene="recR"
                     /locus_tag="ACHKUC_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO6617230.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13960..15834
                     /gene="htpG"
                     /locus_tag="ACHKUC_000937"
     CDS             13960..15834
                     /gene="htpG"
                     /locus_tag="ACHKUC_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO6617231.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMRNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16015..16659
                     /gene="adk"
                     /locus_tag="ACHKUC_000938"
     CDS             16015..16659
                     /gene="adk"
                     /locus_tag="ACHKUC_000938"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO6617232.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16791..17753
                     /gene="hemH"
                     /locus_tag="ACHKUC_000939"
     CDS             16791..17753
                     /gene="hemH"
                     /locus_tag="ACHKUC_000939"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO6617233.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17728..18696)
                     /gene="aes"
                     /locus_tag="ACHKUC_000940"
     CDS             complement(17728..18696)
                     /gene="aes"
                     /locus_tag="ACHKUC_000940"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO6617234.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDRPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYSGMLHAFLHYSRMMKTADDALRDGARFFSDRLQQD"
     gene            18848..20152
                     /gene="gsk"
                     /locus_tag="ACHKUC_000941"
     CDS             18848..20152
                     /gene="gsk"
                     /locus_tag="ACHKUC_000941"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO6617235.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20282..21958)
                     /gene="ybaL"
                     /locus_tag="ACHKUC_000942"
     CDS             complement(20282..21958)
                     /gene="ybaL"
                     /locus_tag="ACHKUC_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO6617236.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22196..23416)
                     /gene="fsr"
                     /locus_tag="ACHKUC_000943"
                     /pseudo
     CDS             complement(22196..23416)
                     /gene="fsr"
                     /locus_tag="ACHKUC_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
     gene            23634..25286
                     /gene="ushA"
                     /locus_tag="ACHKUC_000944"
     CDS             23634..25286
                     /gene="ushA"
                     /locus_tag="ACHKUC_000944"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6617237.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25323..25802)
                     /gene="ybaK"
                     /locus_tag="ACHKUC_000945"
     CDS             complement(25323..25802)
                     /gene="ybaK"
                     /locus_tag="ACHKUC_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO6617238.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVTVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(26006..26800)
                     /gene="ybaP"
                     /locus_tag="ACHKUC_000946"
     CDS             complement(26006..26800)
                     /gene="ybaP"
                     /locus_tag="ACHKUC_000946"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO6617239.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AEKQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPSGRYVVAVGALHLYGEGNLPQMLR"
     gene            26933..27235
                     /locus_tag="ACHKUC_000947"
     CDS             26933..27235
                     /locus_tag="ACHKUC_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6617240.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27271..27612
                     /gene="ybaQ"
                     /locus_tag="ACHKUC_000948"
     CDS             27271..27612
                     /gene="ybaQ"
                     /locus_tag="ACHKUC_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6617241.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27670..30174)
                     /gene="copA"
                     /locus_tag="ACHKUC_000949"
     CDS             complement(27670..30174)
                     /gene="copA"
                     /locus_tag="ACHKUC_000949"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO6617242.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVNTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30436..31368
                     /gene="glsA"
                     /locus_tag="ACHKUC_000950"
     CDS             30436..31368
                     /gene="glsA"
                     /locus_tag="ACHKUC_000950"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO6617243.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKD"
     gene            31371..>31447
                     /locus_tag="ACHKUC_000951"
     CDS             31371..>31447
                     /locus_tag="ACHKUC_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617244.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGA"
BASE COUNT         7724 a         8309 c         8226 g         7188 t
ORIGIN      
        1 gagatcctga gttggtggtt caatacatcc ttaatgttcg taggttatgc ataaaaaagg
       61 ccgcttgcgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg
      121 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
      181 gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc
      241 gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
      301 cgccaacgag gtcatcagga ttgggcgtaa acgcatccgc accgcatcaa gcgtcgcttc
      361 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
      421 gttcttcgcc gacaaaccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt
      481 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
      541 aacggagaac ggaatcgacc agctctcgta cagcgcagcc agacacagga acacgacaat
      601 caacgaaatc gcgtacagtg aaggtgcctg gttgccggag agacgttcct gataggacat
      661 tcccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccatcagctc
      721 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tagatggcag
      781 gccgttgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaacgccga
      841 gaacggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
      901 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
      961 gtcattcaca tagctaccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga
     1021 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     1081 cagaccgttt ggacgtacgc tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa
     1141 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     1201 gtcaaagccg gttgcggtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat
     1261 cgcatctttg atttgcgaga aagcacgtgt tgcacgcatg gtaatcgctt caactttgtt
     1321 ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac cggtattctg
     1381 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     1441 tttggtcaga tagtaattcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc
     1501 acctgctggc agctgaacca tggtcataaa tacgccctgg tcctcatctg gcaagaagga
     1561 gcttggcaga cgcacgaaca gataggccat accgaccacg atgatcagat acagcaccag
     1621 gtaacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     1681 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     1741 ggcaatcggt ttcagcatgg tggcacaaag agccggagtc aggatcaacg ccaccagtac
     1801 cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga
     1861 accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag
     1921 agcgccctga atctgcccca tcgacttacg ggtggcttct tttggcggca aaccttcttc
     1981 cgccatcaca cgctcaacgt tttctaccac aacgatggcg tcatccacca acaggccgat
     2041 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     2101 ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     2161 gttctgcaag aacagataca ttaccaggaa cacgaggatg atcgcttcga ccagcgtttt
     2221 aaccacttcg tgaatagaga ttttcacgaa cggtgtggtg tcatacgggt aaacaatttt
     2281 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     2341 atccagcgcg ttcgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt
     2401 aaactctgcg atgatgtcgt agttctcacc acccagctca attttcgcca catcacgcag
     2461 tagcacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga
     2521 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt
     2581 accaccgagc tgaccagctg caacctgggc gttctgtgct ttgatggcgg taatgacatc
     2641 aaccggcgtt agctggaatt tgttcagttc attcgggttc atccagatac gcatcgcgta
     2701 ctgtgaaccg aacaactgaa cgtcacccac gcccgacgta cggctgatgg catctttcat
     2761 attcgccgcc acgtagtcgg agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     2821 gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgtac
     2881 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     2941 atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtacccg tggagtcact
     3001 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     3061 caccgtgtcc tgcactgttt tcgcatcagc gccagggtag gaggcggaga tcgttactgc
     3121 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     3181 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     3241 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     3301 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     3361 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     3421 acttatcgcc gatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     3481 caaccaccag tacggtggca tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta
     3541 aaatagcgtt tggattaagc ccttcttcca gacgtgcacg cacgaacata cccggcagca
     3601 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa
     3661 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     3721 tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt
     3781 tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct
     3841 gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgtttg
     3901 acttaccaat gcgaccacta atcggagagg tgactttggt gtaagccaga ttgattcgcg
     3961 aagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca
     4021 gagcctgatc gtactcttgc ttactgatgt actgagtacc gagcaatttc tgataacgat
     4081 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac
     4141 tgtcgtatgc cgcctgatag gtcgcaggat caatctgata gagagagaca cctgcttcga
     4201 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     4261 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     4321 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     4381 cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     4441 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     4501 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     4561 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     4621 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     4681 gggggtatca tccacctcgc tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc
     4741 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga
     4801 atccaatatt ggtgaactcg agcttgagta tcaggcaaaa ttccctggcg atccactctc
     4861 agtattaaga gagatattaa ttcatgttct tgagtccacg gtgacagaag aacggcgtcg
     4921 attattgatg gagattatat tccacaaatg cgaatttgtc ggcgaaatgg ctgttgtgca
     4981 acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca
     5041 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgcgcag caattattat
     5101 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     5161 taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac
     5221 gcttcgtaac cctgccacta acgaataacc ctgaatctga ctccaggaat tttcctggac
     5281 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     5341 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     5401 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     5461 acacggcgtt tgcacgggcg tcatcgaatg gtgatttacc gacaaaagcg gacctgcagg
     5521 cacaacttga ctcactgaat aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc
     5581 aggatctgac agatacatta gccaccctcg ataaaatcga tcgcgtaaaa gaagagacag
     5641 ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     5701 taacagcact tagtgatgtc gataacgacg aagaaacgcg caaaattctg agcacgcttt
     5761 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     5821 acgatctggc gtcttataac agccaactgg tttcgctaca gacgcagccc gaacgcgtac
     5881 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     5941 ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag gtccagcagg
     6001 ctttgctgaa tgccgagatt gaccagcagc gtaaaagcct ggaagggaac actgtcttgc
     6061 aggatacctt gcaaaagcag cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc
     6121 agttacaact gttgcaagaa gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg
     6181 cgcaggaagc tgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaagc
     6241 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact caaaacggta
     6301 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgtgcg ctgcaatcgg
     6361 aacgtaatat taaggagcag attgccgttc tgaagggcag tctgctgttg tcacgtatcc
     6421 tgtaccagca acaacaaaca ctcccctcgg cagatgaact ggaaaacatg accaaccgca
     6481 tcgcggattt gcgtctcgaa cagtttgaag tcaaccagca gcgtgacgcg ctgttccaga
     6541 gcgatgcgtt cgtcagcaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     6601 acgatgcatt attgcaagta gttgatatgc gtcgcgaatt gctggatcag cttaacaaac
     6661 agttgggtaa ccagttgatg atggccatta acctgcaaat caaccagcag cagttaatga
     6721 gtgtgtcgaa aaacctgaaa tccatcctga cgcagcaaat tttttgggtg aacagtaacc
     6781 gcccgatgga ctgggactgg attaaagcgt tcccgcaaac gctgaaagat gaatttaagt
     6841 cgatgaaaat cacggtgaac tgggaaaaag cctggcccgc cgtattcatc gccttcctcg
     6901 ctggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctgggc tggctgaaag
     6961 cgtatcaaca aaaactggct tccgctgtgg gttccctgcg taacgacagc cagctcaaca
     7021 caccaaaagc gatccttatc gacctgatcc gcgcactgcc ggtgtgcctg attattctcg
     7081 ctgtcggcct gattctgctg actatgcagc tcaatatcag cgagttattg tggtcgttca
     7141 gcaaaaaact ggcgattttc tggctggtat ttggcctgtg ctggaaagtg ctggagaaaa
     7201 atggtgttgc cgtgcgtcac tttggtatgc cggaacagca gaccagccac tggcgtcggc
     7261 aaattgtccg catcagcctc gcgttgctgc cgatccattt ctggtctgtg gtggcagaac
     7321 tctccccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacctgc
     7381 tgctgattgc cttcctggtg tggccgatgt gccgcgaaag ctggcgtgat aaagagtcgc
     7441 acaccatgcg actggtcacc attaccgtgc tgtcgattat cccgattgcg ttgatggtgc
     7501 tgaccgccac gggttacttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     7561 tttatttggt gatcatctgg aacctgctgt accagacggt attgcgcggc ttaagcgtag
     7621 cggcgcggcg tatcgcctgg cgtcgtgcac tggcgcgtcg gcagaatctg gtgaaagagg
     7681 gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc gctggagcag gttaaccagc
     7741 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
     7801 tttggtctga tttgatcacc gtgttcagct atctcgacag cattacgctc tggcattaca
     7861 acggtactga agctggtgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg
     7921 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttactggaag
     7981 tgctggtgct ctcgcgactg aatatgcgcc agggggcatc gtatgccatc accactatcc
     8041 ttaactacat catcattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctcgt
     8101 gggataaact ccagtggctg gcggcagcat tatccgtagg tcttggtttt ggtttacaag
     8161 agatcttcgg taacttcgtt tccggtttga tcattctgtt cgaacgtccg gtgcgcatag
     8221 gcgacacggt aaccattggt agcttctcgg gtacggtcag caagatccgt attcgtgcga
     8281 caacgattac cgatttcgat cgcaaagaag tgattatccc gaacaaagcg tttgttaccg
     8341 agcgtctgat caactggtcg ttgactgaca cgactacgcg tctggtgatc cgtctcggcg
     8401 tagcttatgg ctccgatctg gaaaaagtgc gtaaagtgtt gctgaaggcg gcgactgagc
     8461 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcattt ggtgccagca
     8521 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg
     8581 atgagctgaa ccgtactatc gatcagttgt gccgtgaaaa cgacatcaac attgccttta
     8641 accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg
     8701 actacaaagg cgacgatccg acaccagcgg ttgggtaata accaaagggg gcgacattta
     8761 gttgccccga gattactaac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct
     8821 tatccggcct acgaaaccgc aagaattcaa tatattgcag gagcggtgta ggcctgataa
     8881 gcgtagcgca tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc
     8941 atttatttct cacttttccg cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt
     9001 tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac
     9061 aatcaaatcg acggccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc
     9121 attgctagcg ggttaaacgc gctaacctgt tttcgatttt ttccagcgca tggcggcagc
     9181 gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtctgctgt tccacgagat
     9241 cggtcgctcg tgccagacgg gctctacgtt cggcaaccat ctcacgtagc cgccgctcaa
     9301 aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggt gcgctatccc
     9361 actcgcgtag cgaccaggct gtggcttcac gcgctatagc ctccagttgc gccgccagat
     9421 gttctgccag ccaggcgact tgcggcagct gttgctgctc aaccgcatga cgaagcgcag
     9481 ccaggttatc gcccgcctca tcgagacaag cctgtagtgt tgtcgcacgg gtctgaaaaa
     9541 ggtgcctgtc aaaacgggca cttagcgtgg caaattgcgc taccggggca caacgctgac
     9601 gcagcgtggc gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac
     9661 ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa
     9721 tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg
     9781 cgtggtatta ccggtattac cgacgacgcc gtttattctg ctggcggcct ggtgctttgc
     9841 ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct
     9901 acgtttctgg cagaaacatc atgcgatgcc gcgcggcgca aaaccacggg cgattttgct
     9961 tattttgatt acgtttacga tttcgctgtg gttcgtccag atgccgtggg tgcgcatcat
    10021 gttgctggta attctcgcct gtttgctttt ttatatgtgg cgaattccgg tgattgatga
    10081 aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta
    10141 cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg
    10201 atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg
    10261 actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa
    10321 cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc
    10381 agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt ggtcggcacc
    10441 gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta
    10501 ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa
    10561 tacggcaccg atcagctgga aatccacgtt gatgccatca aaccgggcga caaagttctg
    10621 gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt
    10681 cgtctgggtg gtgaagtagc tgacgctgcg ttcattatca acctgttcga tctcggcggc
    10741 gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat
    10801 taattatcgt cagtcttgcg ctgctcacgc tacgggcagc acaagatgtg cattcagcct
    10861 cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc
    10921 agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg
    10981 acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta
    11041 ttcatcatgc ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac
    11101 tgttggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg
    11161 ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct
    11221 cgcgcactaa agtcgaagat acccgcgacc tgctggataa cgttcagtac gctccggcgc
    11281 gtggtcgttt caaagtttat ctcatcgacg aagtgcatat gctgtcgcgt cacagcttta
    11341 acgcactgtt aaaaaccctt gaagagccgc cagagcacgt taagtttttg ctggcgacga
    11401 ccgatccgca gaaattgccg gtgacgattt tgtcacgctg tctgcaattt catctcaagg
    11461 cgctggatgt cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa gaacatatcg
    11521 ctcacgagcc gcgggcgctg caattgttgg cacgcgccgc tgaaggcagc ctgcgagatg
    11581 ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggccg
    11641 tcagtgcgat gctgggtacg cttgacgacg atcaggcgtt atcgctggtt gaagcgatgg
    11701 tcgaggccaa cggcgagcgc gtaatggcgc taattaatga agctgctgcc cgtggtatcg
    11761 agtgggaagc gttgctggtg gaaatgctcg gtctgttgca tcgtattgcg atggtacaac
    11821 tttcgcctgc tgctcttggc aacgacatgg ccgccattga gctgcggatg cgtgaactgg
    11881 cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca
    11941 aagaattacc gtatgcgcca gaccgccgta tgggtgttga gatgacgctg ctgcgcgcgc
    12001 tggcgttcca tccgcgtatg cctctgcctg agccagaagt gccacgccag tcctttgcgc
    12061 ccgttgcgcc aacggcagta atgacgccaa cccaggtgcc gccacaaccg caatcagcgc
    12121 cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc
    12181 agcagttgca gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta
    12241 cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg
    12301 ttcaggcgcg cccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc
    12361 gctggaaggc gaccactcca gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc
    12421 tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag
    12481 ccattgagcg cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg
    12541 aacaggtggc gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc
    12601 gctcctctca gcggcatttg aacaaccgcg gcgcacagca aaaactggct gaagcgttga
    12661 gcacgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc
    12721 gtacgccgct ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt
    12781 ccattattgc ggataataat attcagaccc tgcgtcgatt cttcgatgcg gagctggatg
    12841 aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga
    12901 ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag
    12961 cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc
    13021 gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta
    13081 gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca
    13141 gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta
    13201 tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc
    13261 tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg aagtcggcgc
    13321 agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg
    13381 cgctcacccg ggcgatgtcg gaaatcggtc actgcgccga ttgccgcacc ttcaccgaac
    13441 aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa atctgcgtgg
    13501 tggagagtcc ggcggacatc tacgccattg agcagacggg gcagttttca ggccgttatt
    13561 ttgtgttgat ggggcacctg tcaccgctgg acggcatcgg tcctgatgat atcgggcttg
    13621 atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc
    13681 ccacggttga aggtgaagct accgctaact acattgccga gctttgtgcg caatatgacg
    13741 tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg
    13801 gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga
    13861 gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct ccctcatcct
    13921 gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt
    13981 ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat
    14041 aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt
    14101 ttccgcgcgc tttctaaccc ggacctgtac gaaggtgatg gtgaactacg cgttcgtgtc
    14161 tctttcgata aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacccgc
    14221 gacgaagtga ttgaccatct ggggactatc gctaaatccg gcaccaaatc attcctcgaa
    14281 tccctgggct ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc
    14341 tactctgcgt ttatcgtggc tgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa
    14401 ccagaaaacg gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt tgccgacatc
    14461 actaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc
    14521 ctcgatgact ggcgtgtgcg ttccatcatc agcaaatatt ccgaccatat cgcgctgccg
    14581 gtagagatcg aaaaacgcga agagaaagac ggcgaaacca ttatctcctg ggagaaaatc
    14641 aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa
    14701 gagttctaca aacacatcgc ccacgacttt aacgatccgc tgacctggag ccacaaccgc
    14761 gttgaaggta agcaggagta caccagcctg ctgtacattc cgtcccaggc tccgtgggat
    14821 atgcggaacc gcgatcataa acacggcctg aaactgtacg tccagcgtgt gttcatcatg
    14881 gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc
    14941 agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac
    15001 ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac
    15061 gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg
    15121 gaagatttcg ctaaccagga agcgatcgcc aaattgctgc gttttgcttc tacccatacc
    15181 gattcttctg cgcagaccgt atctctggaa gactacgttt cccgcatgaa agaagggcag
    15241 gagaaaatct actacatcac cgcagacagc tatgcggcag cgaagagcag cccgcacctg
    15301 gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg
    15361 atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc taaagttgac
    15421 gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca
    15481 ctgactccgt tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt
    15541 ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc
    15601 actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc
    15661 ttcgaactga acccggatca cgtactggtg aaacgtgcgg cggatactga agatgaagcg
    15721 aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggtacg
    15781 ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat
    15841 gccggatgac cttcgtgtca tccggcattt ttcttttctt catctgcact ttccgcaaat
    15901 tatcttgcca ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg cctgttggtg
    15961 gtatcgttta tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt
    16021 atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag
    16081 aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc
    16141 tccgagctgg gtaaacaagc aaaggacatt atggatgctg gcaaactggt caccgacgaa
    16201 ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgcaa cggtttcctg
    16261 ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat
    16321 gttgattacg ttctggaatt cgacgtaccg gacgaactga ttgttgaccg tatcgtaggc
    16381 cgccgcgttc acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta
    16441 gaaggcaaag acgacgttac cggtgaagag ctgactaccc gtaaagacga tcaggaagag
    16501 accgtacgta aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac
    16561 tccaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtg
    16621 gctgaagttc gcgcagctct ggaaaaaatt ctcggctaat caagccgccg cagatctgct
    16681 ctcgggcagg tctgctcatc ctcctcaggc aatacctctt acagcaatta gttcttcttc
    16741 ttcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaaa
    16801 ctaaaaccgg tatcctgctg gcaaacctgg gtacgcctga tgcccccaca cctgaagcgg
    16861 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt
    16921 tatggtggcc gttgctgcgc ggcgtgattt tgccgctgcg ttcgccgcgt gtggcgaagc
    16981 tgtatgcctc tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac
    17041 agcaggcgct ggcgcaacgt ttaccggaga cgcccgtggc gctgggaatg agctacggct
    17101 cgccatcact ggaaagcgcc gtagatgaac ttctggcaga gcatgtagat catattgtgg
    17161 tgctgccgct ttatccgcaa tactcctgtt caacggtcgg tgcggtatgg gatgaactgg
    17221 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg
    17281 ataaccacga ttatattaat gcactggcga acagcgtacg tgcttctttt gccaaacatg
    17341 gcgaaccgga tctgctgttg ctctcttatc atggcattcc ccagcgttat gcagatgaag
    17401 gcgatgatta cccgcaacgt tgccgcacaa caactcgcga actcgcttcc gcactggaga
    17461 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggtcgggaa ccctggctga
    17521 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtgggt catattcagg
    17581 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
    17641 accgtgaagt cttcctcggc gctggcggga aaaaatacga atatattccg gcgcttaatg
    17701 ccacgccgga gcatatcgaa atgatggcta atcttgttgc agcctatcgc taaagaatcg
    17761 agcaccgtcg cgtagagcat cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc
    17821 gtgcagcata ccggagtaga gtttgaactc acaaggttgc tggtgtgccg ctaacgtctg
    17881 gtaaagcaga cggctgtcat ccagcagtgg atcaaactcc gccccggcaa taaaacaggg
    17941 cggaacttcg cgagtgagat cattattaaa caggcagtaa tatggcgact cgcggtccgc
    18001 gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca
    18061 gacaccaccc aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag
    18121 caaaacgccc gcaactttac cgcaatcgat ctgtttatcg cgcaaccaca acgcactggc
    18181 gagcgccagc atggcacctg cggaatcacc agcaaaacca atgcgggaca tattgatttg
    18241 ataatcctcc gcctgctggt ggaaataaca acaggcagcc acaatttcct ctatcgcttg
    18301 cggaaaacgc gcttcaggtg aaagggtgta atcaatgcca atcaccgtac attggctgta
    18361 gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc
    18421 tccatgcaaa taaaatagcg tcgccggcct atctggctgc ggataaaaga tacgtgtttt
    18481 cacctgccca tattttgttg gaaccctgta agctctggtt gccatttctg gagcgcccac
    18541 attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc
    18601 aggccagggc ggtaaatccg gctgaagagt attcacaacg gtcttcattt cagcagagat
    18661 aaggtccaga acgggtaatt tgttttccgg cttcatgcaa aaactccttt caaattacgt
    18721 cattgtaagg aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg
    18781 cgtctcgcgc gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa
    18841 aacaaccatg aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaatgcacg
    18901 cgatccgctg cttcagcaat tccagccaga aaacgaaacc agcgccgcct gggtagtggg
    18961 tatcgatcaa acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta
    19021 tggattaagc gccgggcatt cattggtgat tgaggatgac gttgccgaag cgctttatca
    19081 ggaactaaaa cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac
    19141 catgcacaac tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag
    19201 caatattgaa attggcagct atgcctatcg ttacctgtgt aacacctcca gccgtaccga
    19261 tcttaactat ctacaaggcg tggatggccc aattggtcgt tgctttacgc tgattggcga
    19321 gtccggggaa cgtacctttg ctatcagccc cggccacatg aaccagctgc gggctgaaag
    19381 tattccggaa gatgtgattg ccggagcctc ggcactggtt ctcacctctt atctggtgcg
    19441 ttgcaagccg ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa
    19501 atataacgta ccggtggtgc tgacgctggg caccaagttt gtcattgccg agaatccgca
    19561 gtggtggcag caattcctca aagaccacgt ctctatcctt gcgatgaacg aagatgaagc
    19621 cgaagcgttg accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt
    19681 agatctggtg ctgtgcaccg ccgggccaat cggcttgtat atggcaggct ttaccgaaga
    19741 cgaagcgaaa cgtaaaaccc agcatccgct gctgccgggt gctatagcgg aatttaacca
    19801 gtatgagttt agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtctattc
    19861 gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcagggga
    19921 tggcgcactg gcagcgttgc tgcatgacat taccgccaac agctaccacc gtagcaacgt
    19981 accaaactcc agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg
    20041 taaatatgct aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg
    20101 cggcttgccg gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt
    20161 cggttcgtag gccagataag gcgttcacgc cgcatctggc attttgcttt caatgcctga
    20221 tgcgacgctg gcgcgtctta tcaggcctac atatccgcgc ctattatccg gcaaccaccg
    20281 tttaccctgt caccacctca cccgccggtg gcgtttccag cagttccagc atagtacggg
    20341 caatttcacg ctcacccatc actacctgat tcgcaccacg ttcggtgata tacgccactt
    20401 catcgtcata atgggcgcgg gcaataatct caatatccgg atttttcgcg cgggcagatg
    20461 ccacaatctc gcccgcttca tagccgttgg gaatcgtcag gatcagccat tttgcacatt
    20521 ccagatgcgc cagttgcata atttcttcgt tcgccgcatt gcccaatact gcgcggaccc
    20581 cgcgctcgcg cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat
    20641 cagaggcgag caatttctcc cccagcaggc tgcctacacg accgtaaccc accagtagcg
    20701 catggttgca aatatccact gggatctgct tctcttcttc gattgcctct tccagcgtct
    20761 gctcttccag cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt
    20821 tgagcataat cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta
    20881 aattcaatgc cattcctagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc
    20941 tggcggcgat ggttaacgcc gtgcgctggg agtgaccaaa caaacgcaca aggaacaggg
    21001 ccgcgaccga tttaccaaac agaataattg ccagcgtcgc cagtactgcc agcggttgct
    21061 gaatcagaat taacggatca aacaacatcc cgacggagac aaaaaacagc accgcgaacg
    21121 catcgcgcaa tggcagtgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta
    21181 ccatcccggc aaagaacgca ccgagtgcaa aggagacatc aaacagctct accgcaccaa
    21241 aggcaatccc taacgccagc gccagcaccg acagggtaaa cagctcgcgg gaaccggttg
    21301 ctgcgctgcg tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga
    21361 taaatgcgat caccttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca
    21421 catcgccctg ttccatcatt cctgctaccg cgggtagcaa taccagtgtc aggaccatca
    21481 ccaggtcttc cacaatcaac caaccgatgg cgatttgccc acgctgactg tcaattaatt
    21541 gccgttcttc aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataagccga
    21601 acacgatacc ggtcattaac gaccagccca gcacggcgga gagcgccata cccagcagcg
    21661 tcgccacggc tatctgggcg atcgcaccgg gaatggcgat ggcctttacc gccatcaaat
    21721 ccttcagcga aaagtgcaga ccgacgccaa acatcaacag aatgacgccc agttcagcca
    21781 gttccggcgc aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac
    21841 ccgctaacag atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga
    21901 taaaggcgag cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc
    21961 cgtctccttt tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta
    22021 atagtttatg acaatttcat tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa
    22081 tggaattagc tgcaaaaaaa gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag
    22141 gcttatggcg actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt
    22201 ctttatgccg gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac
    22261 agattttata gactaactct atgctggtgt ggtcggcgat aagccccaga actgccgccc
    22321 ccagacctcc catgccgaaa gcaaaaccga aaaacagtcc agaaaccata ccgatacgtc
    22381 ccggaagcag ctcctgggcg tagaccagaa tggcagagaa tgctgaagcg aggataaatc
    22441 caataatcac cgttaaaacc cccgtccagt gcaggctggc gtagggtaaa atcagcgtaa
    22501 acggcgcaac gccgaggata gagccccaaa tcacatattt ccgtccaatt ttatccccta
    22561 caggcccgcc aatcaccgta cctgccgcaa cggcaaacag gaaggcaaac aaatgaagct
    22621 gagcattctg gatagataat ccgaattttt gcatcagata aaaggtgtaa tagctgctga
    22681 tgctcgccat atagaaatat ttcgagaaaa tgaggattaa cagaatgctg accgccagaa
    22741 caactttatt gcgcggcagt ggattgataa tcgtcgcttt gggttttcct ttattcattc
    22801 ggtgctgtgc cgagtaccaa cggctgattt gcgccaacac cacgatcgcc agcagtgccg
    22861 caagcacaaa ctaggcaacg ttgcctttgc cataaggcgc gataatcacc gccgccagca
    22921 aggggcccag ggaactgcca aagttgccgc cgacctgaaa gatagattgc gccaggccat
    22981 gacgcccgcc ggaagccata cgggccacgc gagaagattc cggatgaaag accgatgaac
    23041 cggtaccgac cagcgccgct gccagcagaa ctgcgccaaa actgcccgcc agcgccagca
    23101 gcaccagacc acttaaggta aaacacatgc caatcggcaa cgaccacggc atcggatatt
    23161 tatcggtcca gtagccgacc accggttgca gtagcgaaga ggcgagctgg aaggtgaggg
    23221 ttatcatgcc aatctgcata aatgtcagag aaaattctga ctgaagcagt ggataaatcg
    23281 ccagaatcag cgattggatc atgtcgttca gcagatgtga gaggctgata gcacctaaaa
    23341 taccaaacga tgttcgggcc ttagtcgttg acgcagccgc gcccgccaca ggctgggttt
    23401 gttcactcat tgccatagga aagtcacttt ttcagggttg cgatgtaaag aatgatctta
    23461 tttgtgatta ttacccgact aacatacctg tatgcgccgc ctgaaggaag tctcaacgcg
    23521 gaatacagaa tttctaatct ggatgcagat ttatcttcac cggacgcaga cttgtttatg
    23581 atgtcgcgtc atactatttt tcaacacgtt gaaatcaggt cagggagaga agtatgaaat
    23641 tattgcagcg gggcgtggcg ttagcactgt taaccacatt tacactggcg agtgaaactg
    23701 ctctggcgta cgagcaggat aaaacctaca aaattacagt tctgcacacc aatgatcatc
    23761 atgggcattt ttggcgcaat gaatatggtg aatatggtct ggcggcgcaa aaaacgctgg
    23821 tggatggtat ccgcaaagag gttgcggctg aaggcggtag cgtgctgcta ctttccggtg
    23881 gcgacattaa cactggcgtg cccgagtccg acttacagga tgccgaacct gattttcgcg
    23941 gtatgaatct ggtgggctat gacgcgatgg cgatcggtaa tcatgaattt gataacccgc
    24001 tcaccgtatt acgccagcag gaaaagtggg ctaagttccc gttgctttcc gccaatatct
    24061 accagaaaag taccggcgag cgcctgttta aaccatgggc gctgtttaag cgtcaggatc
    24121 tgaaaattgc cgttattggc ctgacgacgg atgacacagc caaaattggt aatccggaat
    24181 atttcactga tatcgaattt cgtaagcccg ctgatgaagc gaagctggtg attcaggagc
    24241 tgcaacagac agaaaagcca gacattatta tcgcggcgac ccatatgggg cattacgaca
    24301 atggtgagca cggctctaac gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg
    24361 gatcgctggc gatgatcgtc ggtggtcact cgcaagatcc ggtctgtatg gcggcagaaa
    24421 acaaaaaaca ggtcgattac gtgccgggta cgccatgcaa accggatcaa caaaacggca
    24481 tctggattgt gcaggcgcat gagtggggca aatacgtggg acgggctgat tttgagtttc
    24541 gtaatggcga aatgaaaatg gttaactacc agctgattcc ggtgaacctg aagaagaaag
    24601 tgacctggga agacgggaaa agcgagcgcg tgctgtacac ccctgaaatc gctgaaaacc
    24661 agcaaatgat ctcgctgtta tcaccgttcc agaacaaagg caaagcgcag ctggaagtga
    24721 aaataggcga aaccaatggt cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga
    24781 ccaatatggg gcggttgatt ctggcagcac aaatggatcg cactggtgcc gactttgcgg
    24841 tgatgagcgg aggcggaatt cgtgattcta tcgaagcagg tgatatcagc tataaaaacg
    24901 tactgaaagt gcagccattc ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg
    24961 tgattgatta cttgaccgcc gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat
    25021 ttgccaacgt tagctttgtg gcgaaagacg gtaaactgaa cgacctgaaa atcaaaggcg
    25081 aaccggtcga tccggcgaaa acttaccgca tggcgacatt aaacttcaat gccaccggcg
    25141 gtgatggcta tccgcgcctt gataacaaac cgggctatgt gaataccggc tttattgatg
    25201 ccgaagtgct gaaagcgtat atccagaaaa gctcgccgct ggatgtgagt gtttatgaac
    25261 cgaaaggtga ggtgagctgg cagtaatccg aaagtgccgg atgttcgcat ccggcacaat
    25321 gcttaatcgc gacgggcgat atcagcaaat ttggcatcga gtatctttgc caggtcgctt
    25381 gccgccagtt cgatatccag accgcgcttg ccgccggaaa cataaatagt ggcaaattct
    25441 tgtgcggggg cgtcgataat cgttggcaga cgttttttct gccccagtgg gctaatccct
    25501 ccaaccagat atcccgtcga acgctgcgcg accatcggat cggccatctc aactttcttg
    25561 gcacccagcg cttttgctac ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc
    25621 acggcaaggt gtttcatatc accgttcact gtgaccagca gcgttttgta gacctgatcc
    25681 ggattcaaac ctaatttttt gacgacttca tcgccaaaat tggtttcagc cggatcgtgc
    25741 tcgtaggtgt ggatttgaaa cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc
    25801 atggtaattt tcctgcctta aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca
    25861 aaaaaaagag tcatcttgcc taagagtatt ggcaggatgg tgagattgag cgacaatcga
    25921 gttacaccgt cgcttaaagt gacggcataa taataaaaaa atgaaattcc tctttgacgg
    25981 gccaatagcg atattggcca tttttttagc gcaacatttg tggcaaattc ccttcgccat
    26041 acaggtgtag tgcaccgacc gcgaccacat agcgccccga cggcatggcg cgtaatttat
    26101 cccgccaggc gagatttcgc tgatgcatca gcacatcgta cagcgactga ctgaacgtat
    26161 tgggcaacgt tatttcatta ttttgcggcg gtgcattcag ccaccagctc atcatttgtt
    26221 gcagcaaccg tgcgttggtg tgccagtggg tcagcgtgtc gtccagcagc gccagtcctt
    26281 tgtcagggag ctggagcagc atggcaattt gcttttctgc gccctccagt tcaatcacgg
    26341 gtttatgttg ttgcttcgcc gcctgcaata gctggtaatc aataccatat tctgcccgca
    26401 gccccagttt ttgcgcctgc gtcgcctgaa gaaccatcgc gatttgccac agcggttggg
    26461 tagaaaagag cgacggagaa atgcccatct cctggctaat gtgctgcagg ttttggagtt
    26521 gctcctcgct aatgcgctct tccagtgcct cgcaggcagg caaattggca aaaggcgtat
    26581 cgctggtgga aacatccgcc tcgacgatca gcgcatcggc gtttttgagc tttttgagca
    26641 aacgggtggg caggggagcc atatcgtggc tacccatatg aatactgccg atcagatgaa
    26701 aatggcgatt gccgggaaga gtgatatcga tagcaggcca ggtgtaatga ttaccgcgca
    26761 gcgcggccca aagcgttttt acccggtaca acagatccat acgacctccc tttgtgaaat
    26821 atcatgctag cgcgcgggga gggatggcgc aaatggagtt gattgttgca cttgcgcaca
    26881 gtgcttgtgt gcattacaat gattgaataa tggacaagga gatgagagag gaatggctca
    26941 aaagaaaaat ataaggagtt ttcgtgatgc ctggttggct gatttttttg tccattcaac
    27001 gccacatcga aagatccctg ctgaaatcca taccacgtta tcacgaaagt tggacatcat
    27061 taatgctgcc acttctcatc gggatttacg ctcccctccg gggaaccgat acgaagagtt
    27121 atccgggaaa ttacaggaat actcctccat tagagtgaat aaacagtacc gactgatttt
    27181 taaatgggtt aatggcaagg ccgaagaatt gtttttagac ccacacaact actaaaacat
    27241 agctgtcgta ttgacgagaa aggtaaaact atgaaacagg caacaagaaa accgacgaca
    27301 cccggagata ttcttctcta tgaatatctg gaaccgctcg atttgaaaat caatgagtta
    27361 gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa caatcgtaaa
    27421 ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt cgatttttgg
    27481 ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg tacccaggaa
    27541 gaattgggac ggattgaaac agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa
    27601 aaggtcgcgt agaataattt ccctataacg gatgcgccac cctaaagcag cgcatccgca
    27661 atgatgtact tattccttcg gtttaaaccg cagcaagcgg ttggcgttac tcactacggt
    27721 gatcgacgag agcgccattg ccgctccggc aaccaccggg ttaagcagtg ttccagtgaa
    27781 cggccacaaa ataccggcgg cgaccggaat accaatgctg ttgtagataa acgcaccgag
    27841 caggttctgc ttcatgttgc gcagcgttgc gcgggaaatt gcgagcgcat ccgcaacacc
    27901 catcaggcta tggcgcatca gggtaatcgc cgcggtttca atggcaacat cactgccgcc
    27961 acccatcgca ataccgacat ccgcctgagc cagcgctggc gcatcgttaa tgccgtcgcc
    28021 caccattgct acctgacgtc cttcactttg cagacgtttg atcgcttcgg ctttaccgtc
    28081 cggcagcacg ccggctatca cctcatcaat ccctgcttct ttagcgatcg cattggcggt
    28141 ggtcgggtta tccccggtta gcatcaccag gcgatatccc gctttatgca ggcgttgcag
    28201 cgccgccacg ctatcactac gcaacggatc gcgtacagcc agcagggcta ctgctttccc
    28261 gtcaaccgcc agcagcacag gcgttgcccc ttgcgatgcc tgagctgaaa tatctgcttc
    28321 aattgctttg gtattgacct gttggtcatt taacaacgcc tgattgccca gcaataacgc
    28381 atgaccttca gcttcaccgc tcacgcccag cccgcgcaat gtgcggaaac cgttgacctg
    28441 cggtagctgc atatcacctg ctttatcgag gatcgcccgt gccagagggt ggctggaacc
    28501 ttgctccagc gccgccgcca gacgcaatgc ttgcgcttca tcaatatcag caaatgtttt
    28561 tactgcgaca acctgcggct tcccttcggt cagcgtcccg gttttatcga acactacggt
    28621 gtcgagcgta ctggcgcgtt gcagcgcgtc ggcgtcccgt accagcacgc caaactcagc
    28681 cgcccgcccg acgccggaaa taatcgacat cggcgtcgcc agtcccagcg cacacggaca
    28741 ggcaataatc agcaccgtgg tggcaatcac cagggtatag acaatctgcg gtgccggacc
    28801 aaagaaatac cagattgccg cactgatcag cgcaatcacc accactaccg gcacaaatac
    28861 ggctgagatt ttatccgcca gctgaccgat ttctggctta ctgctctggg cctggcgcac
    28921 catacgaata atccgcgaca gcgtagtatg gctgccaacc gcactggcgc gaaacagcac
    28981 gctaccgtcc tgcaccactg tcccggcatg gacgctatcg ccttcgcctt tttgctgcgg
    29041 gataggttcg cccgtcagca tcgcttcatc cagccacgct tcgccctggg tgatctcacc
    29101 atctaccggt acgcgatcgc cggtcgtcag gcgcagcaac atacctggct gcacttccgc
    29161 cagcggcacg cttttttcac cttcgtcagt aaccaggcgc gccgtcggtg gggttaaatc
    29221 gagtaatttc tccagcgcct tagaagaacg ctgacgtgcg cgcgcttcca gcatatggcc
    29281 gagattgatc agaccgataa tcatcgcgct ggcttcgtaa taaagatgtc gcgcttccat
    29341 cgggaaccac tgcggccaca ggttgacgct catcgaatag agccacgcaa cacccgttcc
    29401 cagcgccacc agcgtatcca tcgtcgccgc accgttcagc aggcttttcc atgcgctgcg
    29461 gtaaaaatgc ccgccggcga aaaccattac cgccagggtt atcaggccga taactaacca
    29521 caggctgcgg ttgtcagcgg tgaccatcat gttatcgccg atcatccccc agaccatcac
    29581 cgggataccc accgccagcg cgacaattgc ctgccagcgg aagcgcttca tcgtagcgac
    29641 ggcggtttct tgctggcgct cgcggcgttt agcgtcatct tcaatcgctt ccgcgccgta
    29701 gcccgctttt tccaccgcct gcactaaatc ttgtggggag gcactgccca tcaccagcgc
    29761 agtacgctcc gccaggttta cccgtgcctg agtgacgccc ggtacgcttt gcaacgcatt
    29821 ttgtacgcgg gtgacacagc tggcgcagct catgccgctc agcagcaact gctggctgtc
    29881 atcgtcatcg gcggtcgctg ccggaagcgc ctcagaaacc gctgtcagtg cttccgacgg
    29941 gattgatgac tccgccagcg gtttagcctt tgggtggctt acagatgcgt cataacccgc
    30001 ttgtttgatg gtttcgatta gctgttctgc actggcagtc ccggtaacgt gcgcttcagt
    30061 gatagacaca tccgcctgtt caacatccgg gcgctgttcg agactttctt tcacgcgttt
    30121 aacgcagtga ccgcaggaca ggccgtccag ggtcaggtcg atagtttgtg acataaaaca
    30181 ctcctttaag acagttttga ctggctgtga taaaggttaa accttccagc aaggggaagg
    30241 tcaagaaatt aataaaccag gcgggtaaaa gtccgtaaag attaaaaaat cggctcgatt
    30301 tgcatcagga ttagacattt atctctttgt tttcctgtag ttaagttgcg ggtgctaagt
    30361 taaatcagga tgcatgaaaa tcggcaccgg ggtgaggaat tacctcccgc atctataaaa
    30421 aggagttaac aaaagatgtt agatgcaaac aaattacagc aggcagtgga tcaggcttac
    30481 acccaatttc actcacttaa cggcggacaa aatgccgatt acattccctt tctggcgaat
    30541 gtccctggtc aactggcggc agtggctatc gtgaccagcg atggcaacgt ctatagtgcg
    30601 ggtgacagtg attaccgctt tgcgctggaa tccatctcga aagtctgtac gttagccctt
    30661 gcgttagaag atgtcggccc gcaggcggta caggacaaag ttggcgctga cccgaccgga
    30721 ttgcccttta actcagttat cgccttagag ttgcatggcg gcaaaccgct ttcgccactg
    30781 gtaaatgctg gcgctattgc caccaccagc ctgattaacg ctgaaaatac tgaacaacgc
    30841 tggcagcgaa ttttacatat ccaacaacaa ctggctggtg agcaggtagc gctctctgat
    30901 gaagtcaacc agtcggaaca aacaaccaac ttccataacc gggccattgc ctggctgctg
    30961 tactccgccg gatatctcta ttgtgatgca atggaagcct gtgacgtgta cacccgtcag
    31021 tgctccacgc tcatcaatac tattgaactg gcaacgcttg gcgcgacgct ggcggcgggt
    31081 ggtgtgaatc cgctgacgca taaacgcgtt cttcaggccg acaatgtgcc gtacattctg
    31141 gccgaaatga tgatggaagg gctgtatggt cgctccggtg actgggcgta tcgcgttggt
    31201 ttaccgggca aaagcggtgt aggtggcggt attctggcgg tcgttcctgg cgtgatggga
    31261 attgccgcgt tttcaccacc gctggacgaa gaaggcaaca gtgttcgcgg tcaaaaaatg
    31321 gtggcatcgg tcgctaagca actcggctat aacgtgttta aggactgatc atgatgaaca
    31381 cggaaggtaa taacggtaac aaacccctcg gtctatggaa cgtcgtttcc atcggcattg
    31441 gggcaat
//
LOCUS       DAZFRG010000026        31126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFRG010000026 DAZFRG010000000
VERSION     DAZFRG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.026"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..1622
                     /locus_tag="ACHKUC_000952"
     CDS             102..1622
                     /locus_tag="ACHKUC_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HFO6617245.1"
                     /translation="MFAGNKGFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAALAHEL
                     NTLPASVDSTMGVYFIPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAALESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALTPGTVADLKTLLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            1726..2661
                     /locus_tag="ACHKUC_000953"
     CDS             1726..2661
                     /locus_tag="ACHKUC_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6617246.1"
                     /translation="MKLRLTLLTAATLTAISFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANVFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLYN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            2708..3328
                     /locus_tag="ACHKUC_000954"
     CDS             2708..3328
                     /locus_tag="ACHKUC_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 family protein"
                     /protein_id="HFO6617247.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGGITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            3330..4874
                     /locus_tag="ACHKUC_000955"
     CDS             3330..4874
                     /locus_tag="ACHKUC_000955"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6617248.1"
                     /translation="MLPQEIDTPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNK
                     VNVLIGENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNSTHQAEKHGISIIFQELN
                     LFPNMNVMDNIFMANEFFQKGKINEKYQYELAKSLLERLELNVDPYTQLGELGIGHQQ
                     LVEIARALSKDTRVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELME
                     IGDFITIFRDGRFISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTA
                     LHPSGGYKLNDVTFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETID
                     KCHFQARLKKGLALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESA
                     SVKEMILQLAIKVSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAK
                     TDVYQLVGKMAQQGLAVMFSSSELDEVMALADRILVMADGRITADLPRGGVTREKLIA
                     ASTPQD"
     gene            4892..5959
                     /locus_tag="ACHKUC_000956"
     CDS             4892..5959
                     /locus_tag="ACHKUC_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6617249.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGDIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     EALGNTGFATLGSGTFLGVYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            6013..6810
                     /locus_tag="ACHKUC_000957"
     CDS             6013..6810
                     /locus_tag="ACHKUC_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6617250.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAARVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLAHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            6843..7628
                     /locus_tag="ACHKUC_000958"
     CDS             6843..7628
                     /locus_tag="ACHKUC_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6617251.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGGGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(7738..7890)
                     /gene="hokE"
                     /locus_tag="ACHKUC_000959"
     CDS             complement(7738..7890)
                     /gene="hokE"
                     /locus_tag="ACHKUC_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6617252.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            7859..8104
                     /locus_tag="ACHKUC_000960"
                     /pseudo
     CDS             7859..8104
                     /locus_tag="ACHKUC_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8242..8394)
                     /gene="hokE"
                     /locus_tag="ACHKUC_000961"
     CDS             complement(8242..8394)
                     /gene="hokE"
                     /locus_tag="ACHKUC_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6617253.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            8363..8616
                     /locus_tag="ACHKUC_000962"
                     /pseudo
     CDS             8363..8616
                     /locus_tag="ACHKUC_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8840..9958
                     /gene="ybdK"
                     /locus_tag="ACHKUC_000963"
     CDS             8840..9958
                     /gene="ybdK"
                     /locus_tag="ACHKUC_000963"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO6617254.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HHQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10024..10272
                     /gene="ybdJ"
                     /locus_tag="ACHKUC_000964"
     CDS             10024..10272
                     /gene="ybdJ"
                     /locus_tag="ACHKUC_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO6617255.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10337..10720
                     /gene="ybdF"
                     /locus_tag="ACHKUC_000965"
     CDS             10337..10720
                     /gene="ybdF"
                     /locus_tag="ACHKUC_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6617256.1"
                     /translation="MDKQSLHETAKRVALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            10799..11452
                     /gene="nfsB"
                     /locus_tag="ACHKUC_000966"
     CDS             10799..11452
                     /gene="nfsB"
                     /locus_tag="ACHKUC_000966"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO6617257.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            11560..12807
                     /gene="ybdG"
                     /locus_tag="ACHKUC_000967"
     CDS             11560..12807
                     /gene="ybdG"
                     /locus_tag="ACHKUC_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO6617258.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(12888..14264)
                     /gene="pheP"
                     /locus_tag="ACHKUC_000968"
     CDS             complement(12888..14264)
                     /gene="pheP"
                     /locus_tag="ACHKUC_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO6617259.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14366..17509)
                     /gene="cusA"
                     /locus_tag="ACHKUC_000969"
     CDS             complement(14366..17509)
                     /gene="cusA"
                     /locus_tag="ACHKUC_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO6617260.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLLILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17521..18744)
                     /gene="cusB"
                     /locus_tag="ACHKUC_000970"
     CDS             complement(17521..18744)
                     /gene="cusB"
                     /locus_tag="ACHKUC_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO6617261.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(18760..19092)
                     /gene="cusF"
                     /locus_tag="ACHKUC_000971"
     CDS             complement(18760..19092)
                     /gene="cusF"
                     /locus_tag="ACHKUC_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO6617262.1"
                     /translation="MKKTLQVAMFSLFTVIGFNAQANEHHHETMSEAQPKVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGSLSL
                     LQDIKVSQ"
     gene            complement(19116..20498)
                     /gene="cusC"
                     /locus_tag="ACHKUC_000972"
     CDS             complement(19116..20498)
                     /gene="cusC"
                     /locus_tag="ACHKUC_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO6617263.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYFATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPVFNAGRNQANLDIAEIRQQQSVVNYEQKVQNAFKEVADALALRQTLNAQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            20655..21338
                     /gene="cusR"
                     /locus_tag="ACHKUC_000973"
     CDS             20655..21338
                     /gene="cusR"
                     /locus_tag="ACHKUC_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO6617264.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21328..22770
                     /gene="cusS"
                     /locus_tag="ACHKUC_000974"
     CDS             21328..22770
                     /gene="cusS"
                     /locus_tag="ACHKUC_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO6617265.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRNTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDVRGTRFVIILPA"
     gene            22920..25157
                     /gene="nrfB"
                     /locus_tag="ACHKUC_000975"
     CDS             22920..25157
                     /gene="nrfB"
                     /locus_tag="ACHKUC_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO6617266.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVICARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVRKYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            25144..28116
                     /gene="nfrA"
                     /locus_tag="ACHKUC_000976"
     CDS             25144..28116
                     /gene="nfrA"
                     /locus_tag="ACHKUC_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO6617267.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGVTLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQDDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPSIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINITPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            28117..29007
                     /gene="ybcH"
                     /locus_tag="ACHKUC_000977"
     CDS             28117..29007
                     /gene="ybcH"
                     /locus_tag="ACHKUC_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO6617268.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            29655..30608
                     /gene="ompT"
                     /locus_tag="ACHKUC_000978"
     CDS             29655..30608
                     /gene="ompT"
                     /locus_tag="ACHKUC_000978"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO6617269.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DRDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            30852..>31126
                     /locus_tag="ACHKUC_000979"
     CDS             30852..>31126
                     /locus_tag="ACHKUC_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6617270.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAG"
BASE COUNT         7821 a         7975 c         7823 g         7507 t
ORIGIN      
        1 cacaaaatgt cacagcaggg cctgatgaag taataacgtg accatgagga acgcctcatc
       61 tccttccacg ccaggatgca atgtgactca ggagactacc tatgtttgca ggaaataaag
      121 ggttcgttat tgcgttggat gagggtacca ccaatgcaaa agccatcgca cttgatggtt
      181 tcggtaacgt ggtggcgaag ttttctcaac cactggctat tcagacgcca cgtgaaggct
      241 gggttgagca atccggggaa gcgttaatct ctgcctcgct tgatgttatt gcccaggcca
      301 tcaaccacgt gggggcagaa aatgtagcgg ctctggctat cagtaatcag cgtgaaacgg
      361 ctatcggctg gtatcgtcag agtggaaaac cactcaatgc ggcgattacc tggcaatgca
      421 cgcgcagtgc agagttttgt gaaacacttc gtcgcgaggg caaagagcag caaattaaaa
      481 cgactacagg cctgcctatc gccccgctat tttccgcttc caagatgcgt tggctactgg
      541 aatctacacc tgagggtgtg caacttgccg accagggtga aatttgtctc ggcaccattg
      601 atgcctggtt gctatggaac ttgactgctg gcgattcatt ttgctgcgac tactctaacg
      661 ccgcgcgaac tcaattactt aacctgaaaa caggcgagtg ggatgaggaa atgctggcgt
      721 tgtttcacat cccacgcgct gcattgccag aaattaaacc ttccagtggc ctgttcggtt
      781 atacccgagg acttaaaaca attccggatg gtattcccgt catggcgatg gtgggcgatt
      841 ctcacgccgc tctttttggt catgcgttgg gagaagcagg atgtgtgaag gcgacctatg
      901 ggaccggttc ttccgtcatg gcccccgtca actctgctca gtgtgatatc gccacacttg
      961 ccacaaccat tgcctggcat gacggcgaaa atattattta cggtctggaa ggaaatatcc
     1021 cccacaccgg cgatgccgtg gcatggatgg ctgacagcac cggtttaagt gaactgacag
     1081 acgcggcact ggcccacgaa ctgaataccc tgccagcctc agtggattcg actatgggcg
     1141 tttattttat tcctgctctc acaggcctgg gcgcgccatg gtgggatgac agtgctcgcg
     1201 gcgtggtgtg tggcttaagc cgtggcgtaa agcgtgcgca tttgattcgc gccgcactgg
     1261 aatcaatcac ttaccaaatt gcagatgtag tagaagccat gcaacagcat gatgattttc
     1321 acttatcggc gttaatggtg gatggcggcc cgaccaaaaa tgactggttg atgcaatatc
     1381 aggcggatct gctgggttgc ccggtgatgt gcagtgatgt gccagaactt tcggccattg
     1441 gtgcgggtct gctcgcccgc aaagcgctta cccccggcac cgttgcagat ttaaaaacgt
     1501 tattaactga gcacagtgaa tttaaaccgg atatggcgcg tcaccagcga ctgcaaaaga
     1561 gatggcagga gtggcgtcat gcagtgaaca gaacattatg gaaaccggca tcgtcaattt
     1621 gacgaacaag actaaccaac ggaaggagaa taccggccca taaataattc aggctttacg
     1681 ttgtttcacc gtaccagaca ataacaacac agagagaaca ttgtcatgaa attacgttta
     1741 acactgttaa ccgctgcgac cctgactgct atttcttttt ctgcccatgc ggtagaaaaa
     1801 ggaaccatta tgattatggt caattcactg gataatcctt attacgcctc ggaagcgaaa
     1861 ggcgccagtg aaaaagccca gcaactggga tataaaacga ccattctttc tcatggtgaa
     1921 gatgtcaaaa aacagaatga actgatcgat actgcaattg gtaaaaaagt tcagggcatt
     1981 attttagata atgctgactc tactgccagt gttgcagcca ttgaaaaagc aaaaaaagca
     2041 ggtattcccg ttatattaat taatcgcgaa atacctgtgg atgatgttgc actggtgcaa
     2101 attacccata ataacttcca ggctggctcg gaagttgcca acgtatttgt cgagaaaatg
     2161 ggagagaaag gaaaatatgc cgaactcacc tgtaatcttg ctgataataa ttgcgtaact
     2221 cgctcgaaat ctttccacca ggttattgac cagttccctg aaatggttag cgtggcgaag
     2281 caagatgcca aaggcacgtt aattgatggc aaacgaataa tggacagtat tctgcaagcc
     2341 catcccgatg ttaaaggggt gatttgtggt aatggtccgg ttgcactggg agctatcgca
     2401 gcactgaaag ccgccaatcg caatgacgtt attgttgtcg gcattgatgg cagtaacgat
     2461 gagcgcgatg ccgtgaaagc cgaatcattg caggctaccg tgatgttaca agcgcaagcc
     2521 attgccgcgc agggagttac cgatctgtat aattacctgc aaaaaggagt taaacctgcc
     2581 aaacagcggg tgatgttccg cggcattctg ataaccaaag ataacgcaga taaagtccag
     2641 gatttcaata tcaaatcctg atcccaggtc gttgcgccct cacaatgagg gcgcagagga
     2701 gaatattatg gataaaaaag tctggctggc gctggcaatt ctgagcctgg gtggatgtcg
     2761 catcgtctcg cagcaggaac ttgccgatct caaatccccg cctaatcctc atatggcaaa
     2821 tatcgatcag acctggcaga aaaacatcgt gccgcaggtg gtagaaaatg cgcgtccggt
     2881 tgcggagtta atggcggcgc tacaggcaga aaaagatgtc gatgctgcct gtaaaacgct
     2941 gggctatcgt tctcaggaag aaaacccgtg tattttctac gttaaggtcg aaggtggcat
     3001 caccaatatt gatgcgaaat cccgtagcgg aaaaatgacc atcacagata tcagcggcac
     3061 aaatattgtc gtgcagacgg ggccaactct tcgcggcacg cttctgcgag atgcatataa
     3121 aggtgcaagc tatgagcatt ttaacgatca ggttttattt ggtgaatatg gtcgtgctat
     3181 caatgagcaa gccatcaata tgattcagtc ggcaaagctt aaaaccgggg aaacggttca
     3241 ggtttatggc gtattttctg catgggatat tccgcaaact atcccggata taacgcctgc
     3301 gaaaatagcc agggcgggag gcgagtaata tgttgccgca agaaattgac accccccaga
     3361 ctgatgaatc acctgtaatc atagaaacgc atgacttatc gcgtatttat ccgggtgtgg
     3421 tggcgttaga taacgtgaat taccgtgtct atcgcaataa agtaaatgtg cttattggtg
     3481 aaaacggtgc gggtaaatcc acaatgatga aaatgctggc gggggttgaa acaccttcct
     3541 caggcaagat cattcttgat ggtaaagaag ttaccctaaa ttctacccat caggccgaaa
     3601 aacatggaat tagtatcatt tttcaggagc tgaatttatt cccgaatatg aatgtcatgg
     3661 ataatatttt catggcgaat gaattttttc agaaagggaa gattaacgaa aaatatcaat
     3721 atgaattagc caaatctcta ttagaaagac tggaactgaa tgttgatccc tatacccaac
     3781 tgggagagct aggtattggc catcagcaac tggtggagat tgcccgtgcc ctttctaaag
     3841 atacccgggt actgattatg gatgaaccga catcggccct cagccagtcg gaagtaaaag
     3901 tccttttcaa tgtgatcgca caactgaagc gccgtggtgt caccatcatt tatatctccc
     3961 atcgtctgga agagttgatg gagattggcg atttcatcac cattttccgt gatggtcgct
     4021 ttattagtga acgcgccgtg agcgatgcca gtattccgtg gatcatcgag caaatggtcg
     4081 gtgataagaa aaaacatttc gattatcaac cagctacaca aggtgacact gtactggcgg
     4141 tacaggggtt aacggcgctg catcccagcg gtggttacaa actcaatgat gtgacattca
     4201 gcctgcataa aggcgaagtt atcggcattt acggcttgct gggcgcagga cgtactgaac
     4261 tatttaaggg actggtgggc ctgatgccct gtcagagtgg gaagatccag cttaatggtg
     4321 aaaccatcga caaatgccat ttccaggcgc ggctcaaaaa ggggctggcg ttggtgccag
     4381 aagatcgcca gggcgaaggc gtcgttcaga tgatgtcgat tcagtccaac atgacgctat
     4441 ccgatttcag cctgcaaggt tttcgccgtg cctggaaatg gctgaatccg caaaaagaga
     4501 gcgccagtgt gaaagagatg atcctgcaac tggcgatcaa agtcagcgat gccgaactcc
     4561 ctattacctc actcagcggc ggtaaccagc aaaaagtggt acttggaaaa gcgttgatga
     4621 cccagccaca ggtggtgttt cttgatgaac ccacacgcgg cattgatgtt ggcgcaaaaa
     4681 ccgacgtcta tcagctggtg ggaaaaatgg cacaacaggg gctggcagtc atgttctctt
     4741 cttcagaact cgatgaagtc atggcactgg cagatcgcat tctggtgatg gctgatggcc
     4801 gtattaccgc tgatctaccc cggggcgggg tcacccgaga gaaactgatc gcggcttcaa
     4861 cacctcaaga ttaatcaggc tggaatttct tatgaatcag aaatatatga tctatatgta
     4921 cctgttgaaa gccagaacgt ttatcgctct gctgttagtg atcgccttct ttagcgtgat
     4981 ggtgcctaac ttcctgacgg cctctaacct gctgattatg actcaacacg ttgcgattac
     5041 cgggctgctg gcaatcggaa tgacgctggt gatcctgaca ggcggcatcg atctttccgt
     5101 cggcgcggta gcggggatct gcggaatggt tgccggggct ctgctaacaa acggcctgcc
     5161 attgtggagc ggcgatatta ttttcttcaa cgtgccggaa gtgatcctct gcgtggcggt
     5221 atttggcatt ttagtgggac tggtgaatgg tgcggtaatc acccgctttg gtgtcgcgcc
     5281 ctttatctgc accctcggta tgatgtacgt cgcccgtggc tctgccctgc tgtttaatga
     5341 tggcagcact taccccaatc tcaacggcat ggaggcactg ggcaacacgg gtttcgccac
     5401 ccttggttct ggcacgtttt taggcgttta tctgccaatc tggctgatga ttggcttttt
     5461 gattttaggc tactggatca cgactaaaac gccgctcggt cgttatatct atgccattgg
     5521 tggcaatgaa tctgccgcgc gactggcagg cgtacccatc gtaaaagtca aagtgttcgt
     5581 ctatgcgttc tccggattat gcgccgcatt tgtcggtttg attgttgcct cgcagctgca
     5641 aaccgctcac ccaatgaccg gcaatatgtt cgaaatggat gctatcggtg ccacagttct
     5701 cggtggaact gcgctggcag gtggtcgggg acgtgtctct ggctcgatta tcggtgcctt
     5761 tgtcattgtc ttcctcgccg atggcatggt gatgatgggt gtcagtgact tctggcaaat
     5821 ggtcattaaa ggcctcgtta tcgtcaccgc cgtggtagtc gatcagttcc agcaaaaatt
     5881 gcaaaacaaa gtcattttga tgcgtcgcca tgaagaaaag atggccgccg cacctgccgt
     5941 aaaaccgctt tccagttaat taacatcatt ccgccgggca ttaaattgcc cggcttatac
     6001 aggggaaaaa atatgcaacg taattttcag ggtaagactg tagttattac cggggcatgc
     6061 cggggaattg gggccgggat cgctgaacgc ttcgcccgcg atggcgcaaa tctggtgatg
     6121 gtttctaacg ctgcacgcgt gcatgaaacg gctgagcaac tgcgccaatg ttatcaggct
     6181 gacattttgt cattagaagt cgatgtgaca gatgaagcgc aagtacaggc actctatgaa
     6241 caagcggctg cacgttttgg cagcatcgat gtttccattc agaatgcagg cattatcacc
     6301 atcgattatt ttgatcgaat gccggctgct gatttcgaaa aagtgctggc ggtgaacact
     6361 acagcagtct ggctctgctg ccgggaagca gccaaatata tggtgaaaca gaatcacggc
     6421 tgcctgatca acacctcttc tggccaggga cgtcaggggt ttatctatac cccacactat
     6481 gcagccagca aaatgggtgt gatcggcatt acgcaaagcc tggcgcacga gttagcgcag
     6541 tggaatatca ctgttaacgc cttctgccct ggtattatcg aaagcgaaat gtgggactac
     6601 aacgaccgcg tatggggaga aattctcagt accgatgaca agcgttatgg caaaggcgag
     6661 cttatggcgg aatgggtgga aggtattccg atgaagcgtg ccggtaaacc tgaagatgtt
     6721 gccggactgg ttgccttcct cgcctctgac gatgcgcgtt atctcaccgg tcaaaccatc
     6781 aacatcgacg gcggcctgat tatgtcgtaa tgcccctgcg ggctactaag agagatatca
     6841 aaatgatcgc aaaagatttt gctcaacaat tgtttgatct ccacgatcgc gtcgcctttg
     6901 tcactggcgc aggtagcggt atcggacaaa tgatcgctct ggctttagcc agcgcaggcg
     6961 cacgagtggt gtgttttgat ctacgggaag atggcggact caaagagacg gttaatcata
     7021 tcgaagcact tggcggacaa gccttgtatt acacgggcga tgttcgggaa ttgagagatc
     7081 ttcgcgcagc agtcgctctg gcaaaaacac attttggtcg tctggatatt gccgtaaacg
     7141 ccgctggaat cgctaacgca aacccggcgc tggagatgga aagcgagcaa tggcaacgcg
     7201 ttatcgatat caacctgact ggtgtgtgga attcctgtaa ggcggaagct gagttaatgg
     7261 tagagaacgg aggcggttcg attataaata ttgcgtcaat gtccgggatt attgtcaatc
     7321 gtggtctgga acaggcccac tacaacagtt ccaaagccgg cgtgattcat ttatctaaaa
     7381 gtcttgcgat ggaatgggtg agtaagggga tccgcgtgaa ctcaatcagc ccaggatata
     7441 ccgcgacacc aatgaataca cgacctgaaa tggtacatca gactcgggag tttgaaagcc
     7501 agacgccgat tcaacggatg gcgaaagtgg aagagatggc gggtccagcg ctgttccttg
     7561 ccagcgatgc ggcgtcgttc tgcacgggtg tggatctggt ggtcgatggt ggctttgtct
     7621 gctggtaaaa atggtttcgc ccgcagccat cactgaccgc gggcgaaagt gtaaagcagg
     7681 tgccttacca ccctgacctg acaaccggat atgcggggat tactccccgc acagcggcta
     7741 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtaaa
     7801 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgttaaac acagcactat
     7861 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
     7921 taccatccaa attgttcagg tttgggtagg tagccccagg gttgatagaa atatcgtcct
     7981 ggggcttcat ctttccagac ctcagagtta acctgaaacc ggaaagcttc aggcacccgc
     8041 cgttatctta cccttcctca gcaaaatagc tgtttattta tacagcgaat cctgtttagg
     8101 acttttcacc tgtattatcg cccatgccag taaaacgaaa tcgcccgcag ccatctctga
     8161 ctgcgggcga aagtgtaaag caggtgcctt accatcctga cctgacaacc ggatatgcgg
     8221 ggattactcc ccgcacaacg gctacttctt cggttcgtaa gcgagaacag ccttaaactc
     8281 aatattacgt tccttcaccg taaactcaca cagcgagtct ccgaccagaa gcgtaaatcc
     8341 cagcaccgtt aaacacagca ctatgactgc cacaagggca tatttcgtca gcatgtttat
     8401 attgcctcca tgcgaagaag ggactaccat ccaaattgtt cgggtttgga gtggcagccc
     8461 cagggttgat agaaatatct cctggggctt catctttcca gacctcagag taaacctgaa
     8521 accggaaagc ttcaggcacc cgcagttatc ttacccttcc ttagcaaaat agctgtttat
     8581 ttatacagtg agtcctgttt cggacttctc atttaatcaa ctgaagctga acccgttttt
     8641 ttctgcatgc ctcaatcgtc aaaattcttc gccgccagcc agctctgcca taacttgtca
     8701 cattgcaaaa gtagccaaat tgcttcagtc accatcagtt caccatcgcc aaaaaaaaga
     8761 aacattccag aacttccgca tctcacccgg ccgctcccac ttctatactg aatagaaaac
     8821 gccaatataa gagaaaccta tgccattacc cgattttcat gtttctgaac cttttaccct
     8881 cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa gccaggactc
     8941 atcaatgctg attgacgcgg ttaaaaatca gatcacggcc ggagaggtaa agcacgatat
     9001 caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc aggctgccgg
     9061 gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc atctggaaat
     9121 ttgcggcggt ggcacgcacc cgtttcagaa atggcagcgt caggaggtat gcgataacga
     9181 acgctatcaa cgaacgctgg aaaactttgg ttatctcatc cagcaggcga ccgtttttgg
     9241 tcagcatgtc catgttggtt gcgccagtgg cgatgatgcc atttatttgc tgcacggctt
     9301 gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata tgcaaggaac
     9361 ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg ataatggccc
     9421 gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgcc tgagttacac
     9481 cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc ctcattttgg
     9541 cacggtggag gttcgggtaa tggatacccc gttaactctt agccatgcgg taaatatggc
     9601 aggattgatt caggctaccg cccactggtt actgacggaa cgcccgttta aacatcagga
     9661 gaaagattac ctgctgtata aattcaaccg tttccaggcc tgccgctatg ggctggaagg
     9721 cgtcattacc gatccgcaca ctggcgatcg tcgaccattg acggaagaca ccttgcgatt
     9781 gctggaaaaa atcgcccctt cggcacataa aatgggtgca tcgagtgcga ttgaggccct
     9841 gcatcaccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt tcgtcgccga
     9901 tggcggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggctg gtgactaagc
     9961 cgggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac atttaacaat
    10021 gttatgaaac accctttaga aaccttgacc accgcagcag gcattttgct gatggctttc
    10081 ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca aaaactggtg
    10141 accatgttcc atctgatgga tcttagccag ctttacactt tattgttttg tctgtggttt
    10201 ttagtgctgg gcgctattga gtattttgtt ctacgcttta tctggcgacg ctggttctcg
    10261 ctggcggatt aaacgtcgat tatcgccgaa cttcgcggca tactttgctt attctccttc
    10321 gcctaaagga acatttatgg ataagcaatc actgcacgaa acggcgaaac gcgtggctct
    10381 tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg tttttaaaat
    10441 tggcggcaag atttttatgc tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa
    10501 gtccgatcca caaaaatccc tgctaaatca gcaaatatac ccaagcatca agccagggta
    10561 tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct ccgaagcgtt
    10621 acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg ctaaacgcga
    10681 tcaaaaacga ctgcgtcctg actcaaccgt aaatcaatag cgcatttttc tcgcttacca
    10741 tttcatctcg atccttgtaa tctgctggca cgcaaaatta ctttcacatg gagtctttat
    10801 ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg ccagcaaaaa
    10861 acttaccccg gaacaggccg agcagatcaa aactctactg caatacagcc catccagcac
    10921 caactcccag ccatggcatt ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc
    10981 caaatccgct gccgataatt acgtgttcaa cgagcgtaaa atgcttgatg cctcgcacgt
    11041 cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca
    11101 ggaagatgcc gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg
    11161 caagttcttc gccgatatgc accgtaaaga tctgcatgat gatgcagagt ggatggcaaa
    11221 acaggtttat ctcaacgtcg gcaacttcct gctcggcgtg gcggcgctgg gtctggacgc
    11281 ggtacccatc gaaggttttg acgccgccat cctcgacgaa gaatttggtc tgaaagagaa
    11341 aggctacacc agtctggtag ttgttccggt gggtcatcac agcgttgaag attttaacgc
    11401 tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt aattctctct
    11461 tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata accctgtttc
    11521 agccgtcatc atagtctgct gttgtataaa ggagacgtta tgcaggattt aatatcccag
    11581 gttgaagatt tagcgggtat tgagatcgat cacaccacct caatggtgat gattttcggt
    11641 attatttttc tgaccgccgt cgtggtgcat atttttttgc attgggtggt actgcggacc
    11701 ttcgaaaaac gcgccatcgc cagttcacgg ctttggttgc aaatcattac ccagaataaa
    11761 ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca ggcggtattc
    11821 tggctacaaa aaggcaccga agcggcagat attctgacta cctgcgcgca gttgtggatc
    11881 atgatgtatg cgctgctttc agtcttctcg ttgctggatg tgattttgaa tctggcgcag
    11941 aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat taaactgatc
    12001 ggcgcgattc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc gccagcgatt
    12061 ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa agatccgatt
    12121 cttggtctgg tggcaggtat tcagctttcc gcgaacgata tgctgaaact gggcgactgg
    12181 ctggagatgc cgaaatacgg cgcggatggc gcggtgatcg atattgggtt aaccaccgtc
    12241 aaagtgcgta actgggacaa taccattacc actattccca cctggtctct ggtttctgac
    12301 tcctttaaaa actggagcgg gatgtcagca tccggcgggc gacgtattaa gcgcagtatc
    12361 agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg tctgaataaa
    12421 gcgcatttgt taaaacctta tttaaccagc cgccatcagg aaattaatga gtggaatcgc
    12481 cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat tggaaccttt
    12541 cgtgcctatc tgaacgaata tctacgtaac catccgcgga ttcgtaaaga tatgacctta
    12601 atggtacgcc aactggctcc tggcgataac ggtttaccgc tcgagatcta tgcgtttacc
    12661 aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga tcacatattt
    12721 gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa tgatattcgc
    12781 tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tcttatcgaa tggatgagag
    12841 cggtttcgga tggttgacat cattttgtcg gatacggcgt gaatgcctta tttccgacgc
    12901 agcgttttaa atgctacaaa caggaataca atccacaccg gcagcagaat cgctgacaag
    12961 cgcatatcat ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcagaga
    13021 tagttgccga acggataaag cagcgcctta aactgtgttt cacgtccctg acgtcgcatc
    13081 gccgcgcgaa aacgcagatg cgccaggcag atcataatcc agttcaacag cagcgttgct
    13141 accaccagcg ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc
    13201 accagcgaag tgatggctcc ggaaagcatc agcgagttaa tcggcacgcc gcgacggctg
    13261 acgcgagtca aaaacttcgg tgcattaccc tgcacagaaa ggccaaacag catgcggctg
    13321 ttagaataaa ccccgctgtt atacaccgat aacgatgcca ccagaatgac gaagttcagc
    13381 gcagaagcca ccacgttgct gtcgagatta tggaaaatca tcacaaatgg gctactgttg
    13441 gatttcactt ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac
    13501 agcaagatgc gatacaccac ctgattcact gctttaggga tgcttttttc cggatcgcgc
    13561 gcttcagcgg cagtaatccc aatcagctcc agaccgccga aagagaacat aattaccgcc
    13621 agcgacaaaa tcagcccatt ccagccggtg gcaaaaaaac caccgtaacg ccagaggtta
    13681 tcgatactgg ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc
    13741 ataccgatga tcgccagcac tttaatcagc gcaaaccaga attcggtttc gccatataag
    13801 cgcacgttca ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac
    13861 gttggaacat ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt
    13921 cccaccagca cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc
    13981 cagtatttat aggcaaagtg ggcaaatgaa ccagataccg gctcctcaac caccatttcg
    14041 ccaagctggc gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca
    14101 gccggacccg ccatctgaat cgccggacca atgccaagaa acagaccagt accaattgcg
    14161 ccacccagcg caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct
    14221 tgattcgacg cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg
    14281 tcttttttgt tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca
    14341 cacggttaaa ccgggtatcc tgcttttatt tccgtacccg atgtcggtgc agccacatca
    14401 gcttatacgc cgccgggata ataaacagcg atagcaaagg cgcggtgatc atgccgccaa
    14461 tcatcggcgc ggcaatccgg ctcatcacct ctgaacctgc tcccgttccc cacagaatcg
    14521 gcagcagacc agcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg
    14581 cgccgtgata caacgcctca tccagcttct gctcgctgaa tgtttgcgga ttattcaacg
    14641 acggctcggc ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcag
    14701 caaccccggc aagggcaata aaaccggtac ccgtcgccac ggaaagatga aagcccatcc
    14761 accacagcaa ccagatgcca cccaccagcg caaacggtac gctgctgata atcagcaacg
    14821 cttcgcccac ccgacggaac gccagataca acagcacgaa gatgatcatc aacgtcatcg
    14881 gcaccatgag tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg
    14941 ccacgctggt gcccggtttt aactgcactt tttcagctat cgctttttgc agatcgtgaa
    15001 ccaccgacac catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat
    15061 tctcggtttt cagcatcgac ggtccggtag agactttaac gtcagccacg tctgccaggg
    15121 tgatttgctg cttcatcggc gtgaggatca gcaactggcg cagtgcctgc gggctatcgc
    15181 gccagctttg cggataacgc agattaattg gataacgagc aatcccttcc accgtttcgc
    15241 caaccatcgc cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac
    15301 cgtaacgcgc ggctttttca cggttaatct caacgttgat ataacgccca ccttccagac
    15361 gctcagcaag cgcagaagcc acgcctggca ccgttcgcgc cacttcttca atttgctccg
    15421 ccatcgtgtc gatatccgcc agcacagtgc cggaaacttt aatgccaata gggcttttaa
    15481 tgccagtcga gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc
    15541 ccggcagacg cacggtgtta tccagttcct caatgatttt gtccatcgtc atgccaggcc
    15601 gccactgatc ctgcggctta agctggatag tcgtttctac catctccagt ggagctgaat
    15661 cggtggcggt ttccgctttc ccggttttgc caaatacccg cgccacttcc ggtacgctca
    15721 taatcagctt gtcggttttt tgcagcatgc tcgccgcctc tgctgcggaa atccccggca
    15781 gcgtcgatgg catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa
    15841 ctttattgag cggccagaga accgtcagca ccgaaagcgc tgccaccaac agcgtggttt
    15901 tcggccagtg cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga
    15961 gcgggttact gctttccggc ggaattttgc cacggatcca gtagcccatc aggatcggga
    16021 tcactacgat cgccagtagc gccgcgcccg ccatcgcata ggttttggtg aacgccagcg
    16081 ggccaaacag acgaccttcc tgcccttcca gggtgaagat cgggataaac gacagcgtga
    16141 taatcagcag actgataaac agcgccggac ccacttcaac agatgcatcg gtgatcacct
    16201 gccagcgcgt tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc
    16261 gtttatgcgc attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg
    16321 caatgccacc caacgacata atattggcgt tcagtccctg aaagtgcatg acaataaaag
    16381 caatacacaa ccccagcggc aacgaaataa tcgctaccag cgccgagcgc acatgccaga
    16441 gaaacagcgc acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt
    16501 tgtcaatggc gcggtcaatg agctggctgc gatcgtatgt tgtaactatc tccacacctt
    16561 ccggcagact gcttttcagc gtttccagtt tgtccttcac ggcggcgatc acttcacggg
    16621 cgtttttgcc ggaacgcagg atcaccaccc cgcccgccac ttcgccttcg ccgttcagtt
    16681 cggcaatgcc ccggcgcatc tccgggccaa cctggacctt cgcaacatca cgcagataaa
    16741 cgggcacgcc attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca
    16801 gatagccgct ggcgcgcacc atatattccg cttccgccag ttcgatcgac gaaccgcccg
    16861 cttcctggtt tgaagcatcc agcgcgcttt ttacgtcggc gagactgatg ccatactgcg
    16921 ccaggcgctg aggatcgata accacctgat actctttcac cacaccgccc accgacgcca
    16981 cttccgcaac gtcagggatg gtttttagct catatttgag aaaccagtcc tgcaatgagc
    17041 gtaaatcggc cagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc
    17101 caacacccgt ggcatctggc cccagctcgg cgctgactcc cgcaggcagc ttaccctgta
    17161 cctggttgag gtactccagc acccgcgagc gcgcccagta cggatcggtg ccatcttcga
    17221 aaatgacata cacataagag tcgccaaact gcgagaaacc gcgcacagtc ttcgcgccag
    17281 gcaccgacaa catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg
    17341 cctgaccggg atagctggtt ttaataatca cctgtacatc agagagatcc ggcagtgcat
    17401 ccactggcgt gttaatgatg gtccaggtgc cccagatact cagaaacaac gcgcccatca
    17461 gcaccagaaa acggttcgcc accgagcgac gaataatcca ttcaatcatt ggctattccc
    17521 tcaatgcgca tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc
    17581 ttcagaatca atcaggaaca ggccgctgga aaccaccttt tcaccttccg ccagaccaga
    17641 gcgtaacgcg gtgacgcctt gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg
    17701 cccgtcagca tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga
    17761 cggaatgagc agcatcggtt cgctggcggt attgagttgc aaccaggcgt tcatgcccgg
    17821 ttttagcgcc tcgtcggcgt tgtcgacttc cagacgcagt tgcagcgtgc gggtcgcagc
    17881 atccacgcca ggtagcagcg tccatttgcg gatggtgagt gttttatcag gtcgcgccgg
    17941 aacggtgagc gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc
    18001 agcagtgacc cacaccgggt ccataccctg aattttcgcg accacgttat ctttggcgat
    18061 attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt
    18121 aaagcgagtc tggatttttt gcgtggcgat caggcggcga atatcagcct ccggcattcc
    18181 cgccagtcgc agtcgctcaa gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg
    18241 cagcagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt cgagaagcgg
    18301 cgcgcccttt tgcactttat cgcccacggt aagcggatac accttgtcga taaacccggc
    18361 ggcgcgggcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg ccgggaaact
    18421 ctgggaaaaa gtcagcggcc cgcgcgtgac ggtcgccgtt ttcaccccca gattctgcgt
    18481 ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat atttcggcac
    18541 cagatccata tccataaacg gcgatttccc tggtttatcg aaccgcgtat tgggatacat
    18601 cgggtcgtac cagaataaga ctttacgttc tgcggtcgac gttttttctg cgggcggttc
    18661 ctcctttgca aaccaggtaa aacctgccgc agaaataata ccgcccgcga tcatgctgcc
    18721 gataataagc gcgatttttt tcatctcatt aaacccggat tactggctga ctttaatatc
    18781 ctgtaataaa gaaaggctgc cctgctggac aaaattaaac gccactttgt cgccggtttt
    18841 aatttcgctc attttcgtct gcggggtgat ggtaaagcgc atagtcatct ccggccagtt
    18901 cacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat caacaccttt
    18961 taccacgcca gtggcgctaa taacctttgg ttgtgcttcg ctcatggttt catgatgatg
    19021 ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg cgacttgcag
    19081 tgtttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc gccccctaag
    19141 gcggtatata aagaaatttc gttaacctga cgggcataat tcaggtcaag taaagtttgt
    19201 cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac cgcgccgtgc
    19261 tgatataatg cccgcgcccg ttgcaaagta atttgcagcg acgcaagata acgctgctgg
    19321 gcgctgattt gagcgttcag ggtttgacgt aatgccagcg catctgccac ttctttaaag
    19381 gcgttctgga ctttctgttc ataattcacc accgactgct gctggcgaat ttcggcgata
    19441 tccagattgg cctggttgcg tccggcatta aaaacgggga tctcaatttt aggaataaaa
    19501 ttccacatcc cgctgctggc gttaaataat gacgacagat cgctgctggc ggttgatatt
    19561 ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat attggcatta
    19621 gccgccatta acgcgtgttc agcttccata atatcagggc gctgcaataa gatttgcgac
    19681 gacaagcccg ccggtaattt aacgctttgc aggctgtcgc tgtttactgt ctgcgcttgc
    19741 ggcagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg cgccagttcc
    19801 ccctgacgtt tagcgatgtc gctgcgggta ctttctatca ccccgcgagc ctgttccagc
    19861 gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata tgactgctga
    19921 taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagttg ctgattgaaa
    19981 tagctttgcg cgacattaga aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg
    20041 gcaaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa aaaatcgaga
    20101 tcaaagctgg cgttaaggcc agtcgataac tcccgcgtcg tggctgaatc gcctttaaga
    20161 ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc
    20221 agacgatatt gcgcccgcgc ttcctgcact ttcagcgccg ccatgcgcaa atcccggtta
    20281 ttcaccaacg cctcgctaat cagcgtcttc acctgattat caacaaaaaa ggtgcgccag
    20341 cccgcgttct ggtagttatc tgctgcgtta accagaccgt tctggctgag tgagaactgc
    20401 tgcggcacgg gcattgccgg acgctgataa tccggtgcca gtgaacaacc ggttagcgca
    20461 agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt tctggtgatt
    20521 ttatgctgcc aactttactc gccaggctct gattttccgg tgacaggaaa atgacaaaat
    20581 tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt gcccgggcaa
    20641 catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc ggagaatact
    20701 tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac gggctgaatg
    20761 gctaccatct ggcgatgacc ggtgattatg atctgataat cctcgatatt atgctgccgg
    20821 acgtgaacgg ctgggatatc gtgcgcatgt tgcgttccgc caataagggg atgccgattc
    20881 tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag ttgggggcgg
    20941 atgactatct ggtgaagccg ttcgcttttg ctgaactgct ggcgcgggtg cgcactctac
    21001 tgcggcgcgg agcggcggta attatcgaaa gtcagtttca ggttgccgat ctgatggtcg
    21061 atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc agtaaagaat
    21121 ttactctgct ggagtttttc ctccgccatc agggcgaagt gctgccccgc tcgcttatcg
    21181 cctcgcaggt atgggacatg aattttgaca gcgataccaa cgccattgat gtggcggtga
    21241 agcggctgcg cggcaaaatc gacaacgact ttgagccaaa gttgattcag accgtgcgcg
    21301 gcgtgggtta catgctagag gtgccggatg gtcagtaagc catttcagcg cccgttttcg
    21361 ctggcaaccc gcctgacctt ttttatcagc ctcgccacca tcgcggcgtt tttcgccttt
    21421 gcatggatca tgatccactc ggtaaaagtg cattttgccg agcaggatat taatgattta
    21481 aaggagatta gcgccaccct tgaacgggta cttaatcacc ctgacgaaac gcaagcccga
    21541 cgcttaatga cgctggaaga tatcgtcagt ggttattcca atgtgttgat ctccctggca
    21601 aatagccacg gtaaaacggt gtatcactcc cccggtgcgc cggatatccg cgagtttacg
    21661 cgtgacgcca tacccgataa agacgcgcag ggcggcgagg tgtatctcct ttccggcccg
    21721 acgatgatga tgccagggca cggtcacggg catatggaac acagcaactg gcggatgatt
    21781 aacttgccgg ttggcccgct ggttgacggc aaaccgattt atacgctcta catcgcgctt
    21841 tcgatcgatt ttcatcttca ttacatcaat gatttgatga ataaacttat tatgaccgca
    21901 tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa aggtcacgcg
    21961 ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct cgacgttcgc
    22021 cttgacccgc agaccgtgcc cattgagctg gaacagctgg tactgtcgtt caaccatatg
    22081 atcgagcgta ttgaggatgt ctttacccga cagtccaatt tctcagcgga tatcgcccac
    22141 gaaattcgca cacctattac taatctcatt acgcaaacgg aaatcgccct cagccagtcg
    22201 cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagaact gacgcgaatg
    22261 gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca gctaatcccc
    22321 gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt tttcgaggcg
    22381 ttagcagaag atcgcggcgt ggagctgcgg tttgttggcg acgagtgtca ggttgcgggc
    22441 gatccgctga tgctgcgtcg ggcgttaagc aacctgcttt ctaatgccct gcgtaatacg
    22501 ccaaccggag agacaattgt tgtgcgctgc cagacggtcg atcacctggt gcaagttacc
    22561 gtcgaaaacc ccggtacgcc cattgcgccc gaacacttac cgcgattgtt tgaccgtttc
    22621 taccgcgttg atccttcccg ccagcgaaaa ggcgaaggta gcggcattgg cctggcgata
    22681 gtgaaatcga ttgttgtcgc gcataaaggc acagttgcgg taacgtcaga tgtgcggggg
    22741 acaaggtttg ttatcatatt acccgcttaa tccttcagca aacggcaact tttataacca
    22801 gtgtaaaaat aacatgccgc aatagtaata cctattaatg ataaaaagct gtcacaattc
    22861 ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg
    22921 tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta atcgcgataa
    22981 cgttagcggt cattatgttc atcagcgggc tggacgattt ttttattgat gtcgtctact
    23041 gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga atgagttacc
    23101 gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg tggaatgaaa
    23161 cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac gaaaactatc
    23221 atatctttgt tggcacctac cccaacgacc ccgatactca gcgtgatgtt gacgaagtgt
    23281 gcgctcgctt cccgaacgtg cataaggtaa tctgcgcgcg tcctggcccc accagcaaag
    23341 ccgactgtct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc gccaatttcg
    23401 cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg gaattgcgtc
    23461 tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat ccgttcgaac
    23521 gcgaatggac gcacttcacc agcatgacct acattgatga gttttcagaa ctgcatggca
    23581 aagatgttcc ggtgcgtgaa gccctcgccg gacaggtgcc cagcgcaggc gtcggtacct
    23641 gtttcagccg ccgcgccgtg accgcactgt tagctgacgg tgacggtatt gctttcgacg
    23701 tgcagagtct gactgaagat tacgacattg gcttccgcct gaaagagaaa ggtatgacgg
    23761 aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag cgtaaatttt
    23821 tacagcatgc gcggacgtca aacatgatct gcgtgcgcaa atatttcccc gatacctttt
    23881 cgactgcggt tcggcaaaaa tctcgctgga tcatcggcat tgttttccag ggctttaaaa
    23941 cccacaaatg gacctccagc ctgacgctga actactttct ctggcgcgac cgcaaagggg
    24001 caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatacagctt ttgctgttgc
    24061 tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttc agcggcagcg
    24121 catggttaat gactctgctg tggctaaact ttggcttgat gattaaccgc atcgtgcagc
    24181 gggtgatttt cgttactggc tactacggcc tgacgcaggg gctgctatct gtcctgcgtc
    24241 ttttctgggg caacctgatt aacttcatgg ccaactggcg cgcgttaaaa caggtacttc
    24301 aacacggcga tccacgtcgc gtcgcgtggg ataaaacaac gcatgacttc cccagcgtga
    24361 ctggcgatac ccgctcgttg cgtccgttag gtcaaatcct gctggaaaat caggtcatca
    24421 ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc ctgggcggtt
    24481 caatgctgat gcaggggctg attagcgccg agcaactggc acaggcgctg gcagagcaaa
    24541 acggcgtggc gtgggaatcc atcgatgcct ggcagatccc ttcctcgctg attgccgaaa
    24601 tgccggcctc cgtggcgttg cattatgcgg tactgccgct gcgtctggac aatgatgagt
    24661 taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg acgcgtaaag
    24721 tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcacc ggattacgcc
    24781 actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat gcagttcagc
    24841 atcagtggct cacggaacag caggccggtg aaatctggcg gcaatatgtg ccgcatcagt
    24901 tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca attaacgtgt
    24961 tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc accgaaggcg
    25021 ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta caagtttcga
    25081 tgcaatcact attactcaaa gcaggtttaa acactgaaca ggttgcacaa ctggagtccg
    25141 aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact gctgacatct
    25201 ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga gctggggctg
    25261 agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa ggcacagaaa
    25321 aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccagca ggtgccggat
    25381 aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca tgatgaccgg
    25441 gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgatcg gcttgagcgc
    25501 agtctggcgg ctattcctgt tgaagtgaaa agcgttacga ctgttgaaga actgcttgcc
    25561 cagcaaaaag cgtgcgatgc tgcgccgacc ctgcgttgtc gcagtgaagt cgggcagaat
    25621 gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc gacgtttgct
    25681 gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat ctacctgaaa
    25741 caatggtccc aggcagatac gctatataat gaagcacgcc agcagaacac attaagcgcg
    25801 gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agttggatga tcggatcctg
    25861 gcgctgcaat cacaggggat cttcaccgat cctcagtcat atattactta cgcgaccgcg
    25921 ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa taagccgctg
    25981 tttaccacgg acgcacaaga gaaaagttgg ctctatctgt tatctaaata cagcgccaac
    26041 cccgttcagg cgttggcgaa ttatacggtg cagtttgccg ataaccgcca gtatgttgtt
    26101 ggcgtgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca aaaactactc
    26161 gccaccctcc ccgccaatga aatgcttgag gagcgttatg ccgttagcgt agcgacccgt
    26221 aacaaggctg aagctctgcg tctggcacga ttgctgtatc agcaagaacc ggcaaatctt
    26281 acccgcctgg atcaactgac ctggcaactg atgcagaacg ggcagtcacg cgaagctgcc
    26341 gatttattgc tgcaacgcta tcctttccag gacgatgcgc gtgtcagcca gacgttaatg
    26401 gcgcgactgg cgtctctgct ggaaagtcat ccttacctgg caacgccggc gaaggtggca
    26461 attttatcga aacctttacc gctggcggag caacgtcagt ggcaaagtca gttgccgggt
    26521 attgctgata attgcccgtc aatagttcgc ttgctgggcg atatgtcgcc ttcctacgat
    26581 gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg tgtggcgttg
    26641 tatgcatggc ttcaggccga acaacgacaa ccgaacgcct ggcaacatcg tgcggtagcc
    26701 tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca gaaaatcagt
    26761 cttcacgaca tgagcaatga ggatctgctt gctgccgcca ataccgccca ggcggcagga
    26821 aatggcgcgg ctcgcgatcg ctggttacaa caggcagaac aacgtggact gggaaacaat
    26881 gccctctact ggtggctgca tgcgcaacgt tacattccag gtcagccgga actcgcactg
    26941 aacgatctca cgcgctcaat caatattacg ccttctgcca acgcttacgt tgcgcgggca
    27001 acaatttatc gccaacgtca taatatcccg gcggcggtga gtgatttgcg cgccgcgctg
    27061 gaactggaac cgaataatag caacacccag gcagcgcttg gttacgcctt gtgggatagc
    27121 ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct accggacgat
    27181 ccggcactta tccgacaact ggcctacgtg aaccagcgtc tggatgacat gcctgcgacg
    27241 cagcactacg cccggctggt gattgatgac attgataatc aggcgctgat aaccccactg
    27301 accccagagc aaaatcagca acgcttcaat ttccgccgtc tgcatgagga ggtcggtcgc
    27361 cgctggacgt tcagcttcga ttcttctatc ggcttgcgtt ccggcgcaat gagtaccgct
    27421 aacaataatg tcggcggcgc agcgccaggg aaaagctatc gtagctacgg gcaactggaa
    27481 gccgagtacc gcctcggacg caatatgctg ctggaaggtg acctgctctc agtttatagc
    27541 cgcgtctttg ccgataccgg tgaaaacggg gtgatgatgc cggtgaaaaa tccgatgtcc
    27601 ggcaccggtc tgcgctggaa gccgctgcgc gatcagatct ttttcctcgc cgtcgaacag
    27661 cagttgccgc tgaacggaca aaatggcgca tccgatacca tgctgcgcgc cagcgcctca
    27721 ttctttaatg gcggcaaata cagcgacgaa tggcacccga acggttcagg ctggtttgcc
    27781 caaaacctgt acctcgatgc ggcgcaatat atccgccagg atattcaggc gtggacggca
    27841 gattatcgcg taagctggca tcagaaggtg gcgaacggac agactattga gccttacgct
    27901 cacgttcagg acaacggtta tcgtgataaa ggcactcagg gcgcgcagct tggcggtgtc
    27961 ggggtccgct ggaatatctg gaccggcgag acgcactacg acgcctggcc gcacaaagtc
    28021 agtctcggcg tcgaatatca acataccttt aaggcgatta atcaacgtaa cggagagcgc
    28081 aacaacgcgt ttctcaccat tggagtgcac tggtaaatgc gtaagttcat tttcgtattg
    28141 ctgacactgc ttttggtcag ccctttttcc tttgcgatga aaggtattat ctggcaacca
    28201 cagaaccgtg atagtcaggt taccgatacc cagtggcagg ggctgatgag tcagttacgt
    28261 ttgcaaggct tcgataccct ggttttgcaa tggacccgtt acggcgatgc attcacccag
    28321 ccagaacagc gcgcgttatt gtttaagcgt gccgcagctg cgcaacaggc tggcctgaag
    28381 ctgattgtcg ggctgaacgc cgatccggaa ttttttatac accagaaaca gtcctccgca
    28441 gcgctggaaa gctatcttaa tcgcctgctg gctgccgatc tccagcaagc cagattatgg
    28501 agcgccgtgc ctggcgtaac gccggatggc tggtacatca gcgcggaaat tgacgacctg
    28561 aactggcgca gcgaagccgc ccgtcagcct ttgctaacat ggttaaacaa cgcgcagcgg
    28621 ttgattagcg atgtttcagc aaaaccggtt tatatcagta gttttttcgc cggaaacatg
    28681 tcgccagatg gctatcgcca actgctggaa caagttaaag caaccggtgt taatgtctgg
    28741 gtacaggatg gcagcggcgt ggataaactg accgctgaac agcgtgaacg ttatctacag
    28801 gccagcgccg attgccaaag ttccgcccca gcaagcggca ttgtttatga actgtttgtc
    28861 gctggtaaag gtaaaacgtt tacagcgaaa ccgaaaccgg atgcagagat cgcctcgctg
    28921 ttagcgaaac gttcctcttg cggtaaagac acgctctatt tctctctgcg ctatttgccc
    28981 gtcgcgcaga gtatccttga gtattaatga ttaaagggag caacttagct tgctcctttc
    29041 cttttcactt tcctaatcaa aatcctggca agatgaattg tttagcagaa tttctgtttt
    29101 cattttattc gtctgacgtg ttccaattat ttagcaaaac tgcgacaaca aagtgatggt
    29161 gtaatttgta aaagggaaca aataaatggc tcgccctgca ggattcgaac ctgtagccca
    29221 cggcttagaa actccaggaa cgacatttta agtcaacaaa ttaccgcgcc atctctgcgt
    29281 tcacacgtcc cactacctca aaatatgtaa aaccttgcaa gccattgcga ggccttatgt
    29341 gtctcagttt tgttcctctt ttttatacta aaaaacacag caattgagga taaacctcat
    29401 gctattatcg ctaatatgca tctaaaggca tggcacttga atagataaaa actccacaaa
    29461 gaaaaaaata attagcccat aaaaccacaa tgcaaaacac aaacagacaa ttaaactaaa
    29521 aaactgacag tgcattgtga caaaaattca atgttaaaca aaatataaac agtggggcaa
    29581 tatacaattg actcattaag ttagatataa aaaatacata ctcaatcatt aaaacgattg
    29641 aatggagacc ttttatgcgg gcgaaacttc tgggaatagt cctgacaacc cctattgcga
    29701 tcagctcttt tgcttctacc gagactttat cgtttactcc tgacaacata aatgcggaca
    29761 ttagtcttgg aactctgagc ggaaaaacaa aagagcgtgt ttatctagcc gaagaaggag
    29821 gccgaaaagt cagtcaactt gactggaaat tcaataacgc tgcaattatt aaaggggcaa
    29881 ttaattggga tttgatgccc cagatatcta tcggggctgc tggctggaca acactcggca
    29941 gccgaggtgg caatatggtc gatcgggact ggatggattc cagtaacccc ggaacctgga
    30001 cggatgaaag tagacaccct gatacacaac tcaattatgc caacgaattt gatctgaata
    30061 tcaaaggctg gctcctcaac gaacccaatt accgcctggg actcatggcc ggatatcagg
    30121 aaagccgtta tagctttaca gccagaggtg gttcctatat ctacagttct gaggagggat
    30181 tcagagatga tatcggctcc ttcccgaatg gagaaagagc aatcggctac aaacaacgtt
    30241 ttaaaatgcc ctacattggc ttgactggaa gttatcgtta tgaagatttt gagctaggtg
    30301 gcacatttaa atacagcggc tgggtggaag catctgataa cgatgagcac tatgacccag
    30361 gaaaaagaat cacttatcgc agtaaagtca aagaccaaaa ttactattct gttgcagtca
    30421 atgcaggtta ttacgtaact cctaatgcaa aagtttatat tgaaggcgca tggaatcggg
    30481 ttacgaataa aaaaggtgat acttcacttt atgatcacaa tgataacact tctgactaca
    30541 gcaaaaatgg tgcaggcata gaaaactata acttcatcac tactgctggt cttaagtaca
    30601 ccttttaaca acgttaaata gattttctcc ccggtggtat tgtatagccc cggggaattt
    30661 gtttactgtt aaaagcaaac aatatcccct aaccttgtaa gcatttaacc tgcgtcctgg
    30721 cgtgtaacct cttaccgcaa cacaagactc aatatattca aattcgttat gccgtggcat
    30781 catttaaccc cttgtaattc atcgctataa ttgatttaat tcacaaataa aactataaca
    30841 cggtgaaact aatgaaaaaa aacacagatg atggggctaa aatttacaca ccacttaccc
    30901 taaagcttta tgactggtgg gttttgggag tatcaaatcg gcttgcatgg ggatgtccta
    30961 caaaggaaca ccttcttcca cactttctgg aacatttagg taacaaccat ctggatattg
    31021 gcgttggaac tgggttttac cttactcacg tacctgagag cagtctgata tctttaatgg
    31081 atttgaacga agctagcctg aacgcggcat cgacaagggc tgggga
//
LOCUS       DAZFRG010000027        31122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFRG010000027 DAZFRG010000000
VERSION     DAZFRG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.027"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1330)
                     /gene="norR"
                     /locus_tag="ACHKUC_000980"
     CDS             complement(<1..1330)
                     /gene="norR"
                     /locus_tag="ACHKUC_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO6617271.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPP"
     gene            1517..2956
                     /gene="norV"
                     /locus_tag="ACHKUC_000981"
     CDS             1517..2956
                     /gene="norV"
                     /locus_tag="ACHKUC_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO6617272.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            2953..4086
                     /gene="norW"
                     /locus_tag="ACHKUC_000982"
     CDS             2953..4086
                     /gene="norW"
                     /locus_tag="ACHKUC_000982"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO6617273.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIIGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(4310..6562)
                     /gene="hypF"
                     /locus_tag="ACHKUC_000983"
     CDS             complement(4310..6562)
                     /gene="hypF"
                     /locus_tag="ACHKUC_000983"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO6617274.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVAILRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFSLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDVAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAAPCHRVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
     gene            complement(6715..7242)
                     /gene="hydN"
                     /locus_tag="ACHKUC_000984"
     CDS             complement(6715..7242)
                     /gene="hydN"
                     /locus_tag="ACHKUC_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO6617275.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(7391..8401)
                     /gene="ascG"
                     /locus_tag="ACHKUC_000985"
     CDS             complement(7391..8401)
                     /gene="ascG"
                     /locus_tag="ACHKUC_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO6617276.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINDGHQEIAFLTGSMDSPTSIERLAGYKDALAQHSIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            8661..10118
                     /gene="ascF"
                     /locus_tag="ACHKUC_000986"
     CDS             8661..10118
                     /gene="ascF"
                     /locus_tag="ACHKUC_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO6617277.1"
                     /translation="MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFTLIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            10127..11551
                     /gene="ascB"
                     /locus_tag="ACHKUC_000987"
     CDS             10127..11551
                     /gene="ascB"
                     /locus_tag="ACHKUC_000987"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO6617278.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQMKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEMNANNCSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(11649..12068)
                     /locus_tag="ACHKUC_000988"
     CDS             complement(11649..12068)
                     /locus_tag="ACHKUC_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6617279.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(12139..12894)
                     /locus_tag="ACHKUC_000989"
     CDS             complement(12139..12894)
                     /locus_tag="ACHKUC_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617280.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSGSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDSFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQSEDLVLTQRCLIKGDVLDGVNQL"
     gene            complement(12937..13407)
                     /gene="hycI"
                     /locus_tag="ACHKUC_000990"
     CDS             complement(12937..13407)
                     /gene="hycI"
                     /locus_tag="ACHKUC_000990"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO6617281.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE"
     gene            complement(13400..13810)
                     /gene="hycH"
                     /locus_tag="ACHKUC_000991"
     CDS             complement(13400..13810)
                     /gene="hycH"
                     /locus_tag="ACHKUC_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO6617282.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(13807..14574)
                     /gene="hycG"
                     /locus_tag="ACHKUC_000992"
     CDS             complement(13807..14574)
                     /gene="hycG"
                     /locus_tag="ACHKUC_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO6617283.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(14574..15116)
                     /gene="hycF"
                     /locus_tag="ACHKUC_000993"
     CDS             complement(14574..15116)
                     /gene="hycF"
                     /locus_tag="ACHKUC_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO6617284.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(15126..16835)
                     /gene="hycE"
                     /locus_tag="ACHKUC_000994"
     CDS             complement(15126..16835)
                     /gene="hycE"
                     /locus_tag="ACHKUC_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO6617285.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(16853..17776)
                     /gene="hycD"
                     /locus_tag="ACHKUC_000995"
     CDS             complement(16853..17776)
                     /gene="hycD"
                     /locus_tag="ACHKUC_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO6617286.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(17779..19605)
                     /gene="hycC"
                     /locus_tag="ACHKUC_000996"
     CDS             complement(17779..19605)
                     /gene="hycC"
                     /locus_tag="ACHKUC_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO6617287.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(19602..20213)
                     /gene="hycB"
                     /locus_tag="ACHKUC_000997"
     CDS             complement(19602..20213)
                     /gene="hycB"
                     /locus_tag="ACHKUC_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO6617288.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(20338..20799)
                     /gene="hycA"
                     /locus_tag="ACHKUC_000998"
     CDS             complement(20338..20799)
                     /gene="hycA"
                     /locus_tag="ACHKUC_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO6617289.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            21011..21361
                     /gene="hypA"
                     /locus_tag="ACHKUC_000999"
     CDS             21011..21361
                     /gene="hypA"
                     /locus_tag="ACHKUC_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6617290.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            21365..22237
                     /gene="hypB"
                     /locus_tag="ACHKUC_001000"
     CDS             21365..22237
                     /gene="hypB"
                     /locus_tag="ACHKUC_001000"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO6617291.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            22228..22500
                     /gene="hypC"
                     /locus_tag="ACHKUC_001001"
     CDS             22228..22500
                     /gene="hypC"
                     /locus_tag="ACHKUC_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO6617292.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            22500..23621
                     /gene="hypD"
                     /locus_tag="ACHKUC_001002"
     CDS             22500..23621
                     /gene="hypD"
                     /locus_tag="ACHKUC_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO6617293.1"
                     /translation="MRFVDEYREPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASAF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            23618..24628
                     /gene="hypE"
                     /locus_tag="ACHKUC_001003"
     CDS             23618..24628
                     /gene="hypE"
                     /locus_tag="ACHKUC_001003"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO6617294.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            24702..26780
                     /gene="flhA"
                     /locus_tag="ACHKUC_001004"
     CDS             24702..26780
                     /gene="flhA"
                     /locus_tag="ACHKUC_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO6617295.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            26825..27253
                     /locus_tag="ACHKUC_001005"
     CDS             26825..27253
                     /locus_tag="ACHKUC_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO6617296.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRIIALIKASAILIATKA"
     gene            complement(27292..27636)
                     /gene="ygbA"
                     /locus_tag="ACHKUC_001006"
     CDS             complement(27292..27636)
                     /gene="ygbA"
                     /locus_tag="ACHKUC_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO6617297.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            27922..30483
                     /gene="mutS"
                     /locus_tag="ACHKUC_001007"
     CDS             27922..30483
                     /gene="mutS"
                     /locus_tag="ACHKUC_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO6617298.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQHRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            30589..>31122
                     /gene="pphB"
                     /locus_tag="ACHKUC_001008"
     CDS             30589..>31122
                     /gene="pphB"
                     /locus_tag="ACHKUC_001008"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6617299.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAENELL
                     WPVDRVQKSLNSELQKIN"
BASE COUNT         7487 a         8554 c         8202 g         6879 t
ORIGIN      
        1 gcggcggcaa cgtcacctca ggaaaagcaa aatgttgcgc ctcaagaatc acttcatcgc
       61 cgctgcgggt ggctctcgcc agcactaccg cccgatgaat agcatgttcc agttcacgaa
      121 cgttccccgg aaaattgtag tgttgcagta aatttcgcgc tccggcactt aataccactc
      181 gggagagccc cagccgcaaa cgacactgct cgcagaaata ccccgccagc agaatgacat
      241 catcgccccg ctcacgcagc ggcggcactg aaagtggaaa cacgctcagg cggtggaaca
      301 agtcagcgcg aaatcgccct gccagtacct cttcgcgtaa gtcacggtta gtcgccgcca
      361 gcacgcgcac atcgacccgc aaactgcggt catcgccaac gcgttgaata tcgccatact
      421 gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa ctcgccgatc tcatcgagaa
      481 acagcgtgcc gttatccgcc atttcaaact tcccgctgcg gttactgata gcaccagtaa
      541 acgccccttt cacatgcccg aacaactcac tttccgccac actttccggc agtgcggcac
      601 agttgagata gaccagcgga ttcaccgccc gtggcgaggc ttcatgaatc gctttcgcca
      661 ccagctcctt accggttccc gtctcaccgc taattaaaac gttgagatcg gatgccgcca
      721 caatctcaat ctcttttttc aattgcgtca tgcctggcga cagaccaatc atctgcgtct
      781 gtttcaccgc ttcaaacggc gcggcatcgc ctggcagcat attctggctt tccagttgtt
      841 caatcagcaa tgcattgctt aacgctcccg ccgccagtgc ggcaatcagc cgtagctctt
      901 cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc gagcgtcaag gcaccgatca
      961 ggttttgccc ggcaaacaac ggcaaaccaa cgcaggcgtg aaccttcaga ctctcctgcc
     1021 ctggaatcaa accgtcataa ggatcgggca attcgctgtc tgcgggaaag cgcaccacgt
     1081 ccccggcgcg ggcaatcgct tccagccgtg gatgaccttc cagcgcaaaa cgtctaccga
     1141 gtacatcctt cgccagcccg tcgatggcaa gcggaataaa ctgccgcgag tcgtaacgta
     1201 gcaacgcaga cgcatcgcac tccagcacct gacgcagcgt ggtgatcagg cgctgaaaac
     1261 gatcctgatg accaatccca cgctgcaatt cgatggcgat attcgccagc acatcaacgg
     1321 aaaaactcat ctttgcctca ctgtcaattt gactatagat attgtcatat cgaccatttt
     1381 attgatagtc attttgacta ctcattaatg agcacaattt tatttataga gtaaaaacaa
     1441 ttagataaaa aactggcacg caatctgcaa ttagcaagac atctttttag aacacgctga
     1501 ataaattgag gttgctatgt ctattgtggt gaaaaataac attcattggg ttggtcaacg
     1561 tgactgggaa gtgcgtgatt ttcacggtac agaatataaa acgctgcgcg gcagcagcta
     1621 caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc gacaccgtcg accataaatt
     1681 cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat ctggcagata tcgattacat
     1741 cgtaattaac catgctgaag aggaccacgc cggggcgctg accgaactga tggcgcaaat
     1801 tcccgatacg ccgatctact gtacagccaa cgctatcgac tcgataaatg gtcatcacca
     1861 tcatccggag tggaatttta atgtggtgaa aactggcgac acgctggata tcggcaacgg
     1921 caaacagctc atttttgtcg aaacgccaat gctgcactgg ccggacagca tgatgactta
     1981 cctgacaggc gacgcggtgc tattcagtaa cgatgctttc ggtcagcact actgcgacga
     2041 gcatctgttc aacgatgaag tggatcagac ggagcttttc gagcagtgcc agcgttacta
     2101 cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg aaaattaccg agatcctggg
     2161 ctttaacttg ccagtcgata tgatagcgac ctctcacggc gtggtatggc gcgataaccc
     2221 gacgcaaatt gtcgagctgt acctgaaatg ggcggcggat tatcaggaag acagaatcac
     2281 cattttctac gacaccatgt cgaataacac ccgcatgatg gctgacgcta tcgcccaggg
     2341 gattgcggaa accgacccac gcgtggcggt gaaaattttc aacgtcgccc gaagcgataa
     2401 aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg ctggtcggca cttcgacgat
     2461 gaataacgtg atgatgccga aaatcgccgg gctggtggag gagatgaccg gattacgctt
     2521 ccgtaacaaa cgcgccagcg cttttggctc tcacggctgg agcggcggtg cggtggatcg
     2581 tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg cttagcctga aagccaaatg
     2641 gcgaccagac caggacgctc tggagttatg tcgtgaacac ggtcgcgaaa tcgcccgtca
     2701 gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg gtggttgaag aagaaacctc
     2761 tgccgccacg acggctgacc tcggcccacg gatgcaatgc agcgtctgcc agtggattta
     2821 cgatccggca aaaggcgagc caatgcagga cgttgcgcca ggaacgccgt ggagtgaagt
     2881 cccggataac ttcctgtgcc cggaatgctc tctcggcaaa gacgtctttg acgaactggc
     2941 atcggaggca aaatgagcaa cggcattgtg atcattggtt cgggcttcgc cgcccgccaa
     3001 ctggtgaaaa atattcgcaa acaggacgcc agtattccat taaccctgat tgccgccgac
     3061 agcatggatg agtacaacaa acctgacctc agccatgtta tcagtcaggg gcaacgtgcc
     3121 gacgacctta cccgccagac ggcaggtgaa tttgccgagc agtttaatct gcgcctgttt
     3181 ccgcacacct gggtaacgga tatcgatgcc gaagcccatg tggtgaaaag tcagaataat
     3241 cagtggcaat acgacaagtt agtgctggca accggtgcca gcgcctttgt cccgccagtg
     3301 cccgggcgtg agttaatgct gacgttaaat agtcagcaag agtatcgcgc ctgtgaaacg
     3361 caactgcggg atgcccgacg cgtgttgatt atcggcggtg gtttgattgg tagcgaactg
     3421 gcgatggatt tttgtcgggc aggcaaaatg gtcacgctaa tcgacaacgc tgccagtatt
     3481 ctggcgtcgt taatgccacc ggaagtcagc agccgcttgc agcatcggtt gacggagatg
     3541 ggcgttcatc tgctgttgaa atctcagttg cagggactgg aaaaaacaga ttctggcatt
     3601 ctggcaacgc tggaccgcca gcgttgcatc gaagtggatg cggtaattgc cgccaccgga
     3661 ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga cgattaatcg cggcgtttgc
     3721 gtcgatagtt atctgcaaac cagtaatgcc gatatttatg cgctgggcga ttgcgcggaa
     3781 attaacggtc aggtattgcc gttcctccag ccgattcaac ttagcgcgat ggtgctggca
     3841 aaaaatcttc tcggcaataa cacgccgctg aaactcccgg cgatgctggt gaaaatcaaa
     3901 acgccggaat taccgctgca tctggcaggc gaaacccagc gtcaggattt acgctggcaa
     3961 attaataccg aacgccaggg aatggtggcg cgcggcgttg acgatgctga ccagcttcgc
     4021 gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg gattgttgaa aacgttgtcg
     4081 atgtaggcga gctacagtgc cccaaatgtc ggatgcggcg ctgacgcgtc ttatccgacc
     4141 tacagggaac gcatgtgttg atcacataat acgtttatgt cgcatcctgc actgtcccaa
     4201 aatgtcggat gcgacgctgg cgcgtcttat ccgacctgcg gggatgaata tgtaggccgg
     4261 ataaggcgtt cacgccgcat ccggcaatgg tgtccaaatg cagcacgttt tatccgttct
     4321 ggacttcccc tgccaaccaa cgcgccgcaa caataacccc ctgccccaga gacaacccgc
     4381 catcgcccgc aggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
     4441 gacgtgcacg cagcaaacgg ttatgaataa ctccgccgct aaataccagc gtagtgatac
     4501 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
     4561 acgcccacgc gcgttgatta accggagcct gccagttcag ccactgctgc cagaaagtgg
     4621 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ctgtggcacg
     4681 gagctgcgag tgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
     4741 cacagcccag cgccgccgct acggcatcga acaaacgtcc acacgacgat gccagcggcg
     4801 cgttaattcc acgctcaata gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
     4861 ccgtttcaga gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
     4921 gccacggctg cttcgcagcc acatcgcccc ccggaagcgc caccgcgggc aagccgccca
     4981 ggtgctggca ttcacgatag ttcacccgca agcactcgcc cccccataaa gcgccgttct
     5041 cccccatacc aataccgtcg agcgtcaacg caatgacatc tccgccatcc agaggccaga
     5101 ggtgttctgc cagacacgcc gctgcgtggg catgatgatg cagtaccgtt tgcgtcggca
     5161 gattcatttc gcgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
     5221 attgcggagt gaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
     5281 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcgccgcgca
     5341 ccaggctgaa ggtgtttttc agatccgcac cgagacacag cacaggcgga acatttttaa
     5401 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
     5461 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
     5521 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gccggtggtt
     5581 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
     5641 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggccaggg gcgatatcat
     5701 cacaaagctc aggtacatat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
     5761 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
     5821 tcgccgggcg atgtttgcgc gcccgaagta tcgccaccgc gttactgtta cgtgcatcgc
     5881 aggcaagatg aaatccgcca attcctttga tggcgacaat gttgcccatt tttaactgtg
     5941 cgatagctgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
     6001 gatgggggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
     6061 cacagtactc tttgtcacaa gccggacaca gcggaaacgc cgccatcacg gtaaacgggc
     6121 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc acagtgggta cagttgataa
     6181 acggataacg ccagcgtcgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
     6241 tggcggcatc gggaacaatt tgcgtattca tgacgccacc cgcgctctgg cggatggtga
     6301 actcagtggg cagttgtgac cagataaacg gctcacgctc gacgctatca atacgcgcca
     6361 gtggcgggca gtgctgatgc aattgaacaa gaaacgtttc cggatcttcc agcagccgga
     6421 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagtt
     6481 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
     6541 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgccc
     6601 agccggacac gacagcgtcg catccggcag tcacaggtcg gcgatactgc ccgcttcgta
     6661 ttctacgaat atttccggga attcctttga taccagaaca gttctgtaag atttttagaa
     6721 catcagcgcc gtgcggcggc gtttttctgc gctcagttgt tcaagtttat tacgatcgac
     6781 acaaatcagc gcatgggtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
     6841 gcacaggtcg catttattgg cttcggcttt gtcagcccgt acgttaagcc ctgcgccgct
     6901 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
     6961 ggttttgcaa ccaatgcagc gttcctgcat cacatgaaca aaccctttat cacggctgat
     7021 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc agactgttgc
     7081 cgtagaaatg ttcacacctt taatgacatg gattcgcggt aaaaaagttt ccggggtcag
     7141 cgatgcacag tcctgattct cctgatgaga aaccacgcac gccacttcac aggtacggca
     7201 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
     7261 cagttaatgc gccgatacat tcacaaatca tgccagtttt taatttactg ttatttaagg
     7321 aaattaaatt ctgtattaca ggaaaaacga tgtcatcgac actagtgacg atgacatgtg
     7381 atgacaatgt ttatcgcgaa agagcaatga gtgagtcgcg gcggatcagt tttccgctga
     7441 aggttttcgg cggtgagaaa tccccgccat cgagcataaa aatcagccgt ccaataattt
     7501 cctgaatcat ctcagtcacc ggaattttta cgctggagag cgccggaacg atgtaggggg
     7561 caattgcgat atcatcgaat ccgataactg acacctgctc tggcaccgct acgccgcgct
     7621 cgtgtaacgc tttgatcgca cctatcgcca tatcgtcgtt actggcaact aacgcgctaa
     7681 atttagcccc acgttcgagc aacgtttcta ccccttcggc cccgctagcg ggcgtccatt
     7741 taccgttagc gataagtttt tcattgagag caatactatg ctgcgccagc gcgtctttat
     7801 atccggcaag gcgttcaatg ctggtggggg aatccatcga accggtaagg aaagcaatct
     7861 cctgatgtcc gtcgtttatc aactctgcca cggcgttaaa gctggtctgt ttatgatcgc
     7921 accagacgct atggctgctg tttttgcgca ggcggcgatt aagcaccatt atcggctgac
     7981 tgtgtgcgtc aatgatatca tcaatctcat ccacgcttaa aaagcgcgga taaatcatga
     8041 tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg gcgctcttct tcggcgctat
     8101 gtttaccatc tgccaatagc aactgtcgcc ctttctcttc cgccattcgc gcggcatgaa
     8161 acagtaattc actaaaataa atgccatggt aaagcgtgtt ggtcactacc agccccagcg
     8221 tctgagtact cttcgccgac agattgcgcg ccagcaagtt tggacggtaa ccgctctctt
     8281 ctaccgcctg aaacacgcga tctttagtct cctggctgac gtagccatta cctgaaagca
     8341 cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca
     8401 tcattttcat ccctttacac gcaatcaacg cagtgtactg caccgtttgc cgattgtcct
     8461 tgcgcaatcg gcgggaaaaa tattcaggtg accggtttca caaatataaa aaatgaacaa
     8521 ttaactctct tgcttattgc atgttaacta ttcatgattt tgtgaaaccg gtttcctaat
     8581 tccgtttcag catcggcatt tttccgtcac gtcgactgat aacaactaca tctaccctac
     8641 tgataacagg ataaaatccg atggccaaaa attatgcagc gctggcaagc tcggtgataa
     8701 cggcactggg cggcgttgat aacatctcgg cggtcacgca ctgtatgacg cgtttgcgct
     8761 ttgttatcaa agatgaccag cttatcgaca gtccgacgtt gaaaaccatc tccggcgtgc
     8821 tcggcgtggt acgtagtgac aaccagtgtc aggtgattat aggtaatacg gtttcgcagg
     8881 cctttcagga agtcgtcagc ctgctgccgg gagatatgca gcccgcacag cccgtgggta
     8941 aacccaaact tacgctacgt cgcataggtg cggggatcct cgatgcgctg atcggcacca
     9001 tgtcaccgct gatcccggcg attatcggcg gatcgatggt caaactgctg gcaatgatcc
     9061 tcgagatgag cggcgtgctg acaaaaggat cgccgacctt aaccattctg aatgtgattg
     9121 gtgacggtgc tttcttcttc ctgccgctga tggtcgctgc ttctgccgcc atcaaattta
     9181 aaaccaatat gtcgctggcg attgccatcg cgggtgtgct ggtacatccg agttttattg
     9241 aactgatggc gaaagcggcc cagggtgaac atgtcgaatt taccctgatc ccggtcaccg
     9301 cggtgaaata cacctacacg gtgatcccgg cgctggtgat gacctggtgc ctgtcatata
     9361 tcgaacgctg ggtggacagc attacaccgg cggtgacgaa aaacttcctc aagccaatgc
     9421 tgattgtgtt gattgccgca ccgctggcaa tcctgctgat tggcccgatt ggtatctgga
     9481 tcggtagcgc catttcggcg ctggtttaca ccattcatgg ttatctgggc tggctttcag
     9541 tcgccattat gggcgcgctg tggcctctgc tggtaatgac agggatgcac cgcgtcttta
     9601 cgccaaccat cattcagacc attgccgaaa ccggcaaaga agggatggtc atgccgtcag
     9661 agatcggcgc taacctgtcg ctgggcggtt catcactggc ggtggcatgg aaaacgaaaa
     9721 acccggaact gcgccagacg gcgctcgctg cggcggcatc agccattatg gcggggattt
     9781 ccgaaccggc gttatacggc gtggcgatcc gcctgaaacg tccgcttatc gccagtctta
     9841 tcagcggttt tatttgcggc gcggttgctg gtatggcggg gcttgccagc cactcaatgg
     9901 cagcgccggg gctatttacc agcgtgcagt tcttcgatcc ggcgaatcca atgagcatcg
     9961 tctgggtgtt cgccgtcatg gcgctggcgg tcgtgctgtc gttcatcctc acactgttgc
    10021 tcggctttga ggatattcct gttgaggaag cgactgccga ggcgcgaaag catcagagcg
    10081 tacaaccgac cgtcgccaaa gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc
    10141 agaaggtttt ttatggggcg gcgcgcttgc cgccaaccag tctgaaggtg cgtaccgtga
    10201 aggtggcaaa gggctgacca ccgtcgatat gatcccacac ggcgagcatc gaatggcggt
    10261 gaaactggga ctggaaaaac gttttcagtt gcgcgatgac gagttttatc ccagccatga
    10321 ggcgacggat ttttatcatc gttataaaga agatatcgcc ctgatggcag agatgggatt
    10381 caaggttttc cgtacctcaa ttgcctggag ccgcctcttc ccgcagggcg atgaactgac
    10441 gccaaaccag cagggcattg ctttttaccg cgcggtattt gaagagtgta aaaagtacgg
    10501 tatcgaaccg ctggtcacgt tatgccactt cgatgtgccg atgcatctgg tcaccgaata
    10561 tggctcctgg cgtaaccgca agctggtaga gcttttcagc cgctacgccc ggacctgctt
    10621 tgaagcattt gatggtctgg tgaaatactg gcttaccttc aatgaaatca acattatgtt
    10681 gcatagcccg ttctccggcg cgggtctggt gtttgaagaa ggggaaaatc aggatcagat
    10741 gaaatatcag gctgcgcatc accagctggt tgccagtgcg ctagccacca aaatcgccca
    10801 tgaggttaac ccgcaaaatc aggtgggttg tatgctggcg ggcggtaact tctaccctta
    10861 cagctgcaag ccggaagatg tctgggcggc gctggagaaa gatcgggaaa acctgttttt
    10921 tatcgatgtg caggcgcggg gcgcgtatcc ggcttactct gcccgcgtat tccgcgaaaa
    10981 aggggtaacc atcgacaaag caccgagcga tgatgagatc ctgaaaaaca ccgtcgattt
    11041 tgtctctttc agctattacg cctcgcgctg cgcctcggcg gagatgaacg ccaacaactg
    11101 cagtgcggcg aacgtggtga aatcgctgcg taacccgtat ctgcaagtga gcgactgggg
    11161 ctggggtatt gatccactcg gtctgcgtat caccatgaat atgatgtacg accgttatca
    11221 gaagccactg tttctggtgg aaaacggcct gggcgcaaaa gatgaatttg ctgccaatgg
    11281 cgaaattaac gacgactatc gcatcagcta tttacgcgaa catatccgcg caatgggcga
    11341 agcgattgca gacggcattc cgctgatggg ctacaccaca tggggctgta ttgatttagt
    11401 ttccgcctct acgggtgaaa tgagcaaacg ctacggtttt gtctacgtag accgtgacga
    11461 cgcaggcaac ggcacgctga cgcgcacgcg taagaaatcg ttctggtggt ataaaaaagt
    11521 gattgccagt aatggtgaag atttagagta agtaaccgtg ccggatgcgg cgtgaacgcc
    11581 ttatccggcc tacaaggccc gataagcgtt gcgcatcggg caatcttggg tttggcatca
    11641 gtcaaaaatc aaagaaacca ttccggctta acgccaaatc gggctgaaag tgccttaatg
    11701 tgggatatcg tcagggatcg ttgtccagac aagatctggc tgaccaggga tttagagccg
    11761 atttcgttct tcaaatccgc ataggtcaga ttatgttgat caatcagcgt acgaagtaac
    11821 gccacgccaa ccggcattgc tgcaacggct ttgtcgaact ctgcaaactt ttcgttattg
    11881 ttttcatatt ctgcaatgcg gctcgccaga aagtcgataa gtggattggt gtcatcttct
    11941 tcaatcaaat attcgaccag ttccagtgct tcacgataat catcttctga gtcactgcct
    12001 cccagaaagg ggactgcatt gaccagttct cttgttgctt taacagcgcg tgcagcatta
    12061 gcagtcattc tttgttctct ctgtagtaac gtgacatctg tctacacata atccccctca
    12121 tagctactct gccaaaagtc agagctggtt caccccatca agaacatccc cctttatcaa
    12181 gcaacgctgc gtcagcacca gatcttccga ttgcgggtcg cgcccggcga tgggtttgag
    12241 gacgagttta cggttggggg tataagtaaa acgcgtgagc gcgggatagc ttaacttgcc
    12301 aaaccagccg tgaacacgct gccatttggc gtcttttacc tgatacagac tggttatcca
    12361 gtaaccggaa tcaaactgca tatccagcaa ttgcgtgtgg ccattccttt gtaaatccag
    12421 caaatcatca atgccagttt cacttgcggt ataaaaacta tcggactgaa aaacacacgg
    12481 gcgaccttgc tgattaaagg tcatcagaat caaaaacgcg ttaggtgcca gaccatttcc
    12541 tgaaataccc cgccagataa ccagatcctg aatgccgtta cgatcaagat cggcacggta
    12601 aagttgcgca ttacccgcca tattttgcag cacccccaca ggcacagacc cggatttacc
    12661 ggcgcgatct ttaccgttca attgccattc actatcatca tttggcatct tttcaagcgt
    12721 cacgccagca acgccaactt ttctaaaatg ccagttttgc tcggcatcat aaggcatggt
    12781 caggagaggt ttcagcgccc gcttttgcgc gtattcgctg tcactcatcg gcagatattc
    12841 ggtaaagctg gtggcctgtg caggcagcac taagctcaat aaaaaagcgc acatccctgt
    12901 tctgatgttc aattccctga attccttact gaaaaactac tcttcttccg ccgctaactg
    12961 cgcgaagccg ccatttcctt cccagccttc cagccgctga tacaccgttt ccaccgcatc
    13021 tttaatctgc tgagtcatcg ggtagtaaaa gccgacgata tccggctgga tgccaaggaa
    13081 aatcacttcg ccaatatctt ctttcaactg gtcgataaga tagttcagcg gcatgttatg
    13141 ggtagtcatc ataaacatct cggcgatgtc agccgggtcg atgatgcgga tctcgccagg
    13201 gtttagcccc atatccgtgg cgtcgacaat aagcaaacgt gtcgggcgca gttcgcggat
    13261 agcgacaatg tcgttttccg gtgcgctacc gccgtcaatc actacccagt tacctttcgg
    13321 cgcggcagcg cacttttccg ccagtagcgg gcctgcgcca tcatcaccca tcatgctatt
    13381 gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag atggcgtttt
    13441 cctgatgaat atcatgcagc atacttaaca gcattgtact ccattccttc tgctgcgaag
    13501 tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg ttaatgtcga
    13561 tgacgatctc accatatttc ggcacgcctt ccattttgcg gcgggcttcg ctgcccgcct
    13621 ccagagtggc aatccatgcc agatagtcat cccacgggca ggtaagcgcc gcttccaggc
    13681 aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc tgctgcgcct
    13741 cggcgggcgt agcatcgttc tcatcaatga atttacggct cagttgactg aacaccacct
    13801 tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga caatctcgtt
    13861 cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc cctgccctaa
    13921 ttgcgtcagg aaatcatcgg caatctggcg accgtaacga taacccgcca ggcggcgtgc
    13981 ttcgcgatcc actttcacgc gcagcggctg caccatatcg ccatgcagga tctccgccgg
    14041 ttgttcatcc tgctcacccg gcccacgggc gtggattttc tgctccagca ggccgagcgc
    14101 catcgcaaag ccgtacagcg tggcggcagg cgttggcggg cagccaggga tataaacatc
    14161 cactggaacg attttatccg taccgcccca cacgcagtag agatcgtgga agatcccgcc
    14221 actgttaccg caggcaccgt aggagataca gattttcggg tccggcgcgg actgccacgc
    14281 acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta aaatatccgc
    14341 atgacgcggt gacggaacga ctttaatgcc gaagcgttct gcatcgaaca gcggcgaaag
    14401 cgtggcgaaa atttcgattt cgcaaccgtt acagccgccg cagtccacgc gataaacgta
    14461 ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg attcatccac
    14521 cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc tcatcagatg
    14581 gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca tttctggcgc
    14641 ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct gtcaccgttg
    14701 tgcttaagca gcgcaatggc gtagtcgatc tctttctgga cggcgaaagg acgattgcag
    14761 acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt cttccacacc
    14821 gccagttcgt actcttgcga cagcttaatc gccgctgtcg ggcagacttc ttcgcagcgc
    14881 ccacagaaga tgcagcgccc aaggtcaaac tgccacgcca gctcaccagt ggcgaggtcg
    14941 gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aagccgcaca gccaatgcac
    15001 tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat cggctccagc
    15061 ggataagacg aagtcgccgt gccggttttg atgacttttt tgataaaggt aaacatggcg
    15121 attccttatt tcagcggcga gtttttacgc tcaatgctgt agcgctcaag ttctttgtac
    15181 ggcaccactt tgctcttctt cttacgtaca tcgaccacgg tcatgcggtc ggtacaggag
    15241 tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtgtt gccgcgcagc
    15301 atataacgca gggtcggcca gttcgcgtag gtcgccgcac ggcagcgcca gcggtacagc
    15361 ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc ttcggcaaag
    15421 cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg gccgcccggc
    15481 aggttatcca gaccgtaatc gatcatattc agcgcggtat agacttcgtt gatacgcact
    15541 ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg cagcaggcca
    15601 taaccaacaa acgggtgatc ggcacgggta tcacgggcgt gaccgctggc acgaaccatc
    15661 gggccaacgt tactgaagtc gcgagcgatt tccgggtcca gacgaccaat gccgacggtg
    15721 cgttgttcca tgttcggtgt gctcagcagc acatccacca gctcctgcac ttcacgacgc
    15781 atctgttgtg ccagctggcg ggtctggatc atgtcgtctt tcagcagatc gcgacgaatc
    15841 ccgccgatca ggttcaggcc gtaggtttta cgtgcaccgg taaggatctc tgccattttc
    15901 atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc ggtaaagtga
    15961 caggccaggc cgagattgag cagatgcgag tgcaggcgtt ctacctccag cagaatggcg
    16021 cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac cgacgtggta
    16081 taggcggtgc tgtgagcaaa gccgcagatc ccgcacacac ggtcagagag gaaggtcact
    16141 tcgttgtaac ccatacgggt ttccgccagt ttttccatgc cgcgatggac gtagaacagg
    16201 cggtagtcgg cgtcgataat gttttcacca tcgacgaaca gacggaagtg acccggttcg
    16261 tcagaagtga cgtgcagggg accaatcggc acgacgttgt ttttcttgtc gcccagttcg
    16321 ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg ataatccatg
    16381 ctgtctttac gcagaggata aagttcatcc ggccagtcat ccggcagcac cagacgacgt
    16441 tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcacg ctcgccccac
    16501 accgccgccg gaacgcgcgg cgtcacggaa ggatactccg gtttgttggc gtcaacttcg
    16561 acgcgaaccg ttacccaaca cttggtgccc ttctccatcg acagcacgta ataaacggcg
    16621 taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca acccccttgt
    16681 ttgtagtaga gaaactccac cacttccggc aggtagttca ccttcacggt gacggttagc
    16741 tgatctttgg tctgccaggc gtggtccagc acgacgccag gaaatgcctc attcagcgcg
    16801 gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt aatcacgccg
    16861 ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc ggcccaggta atgcgcggag
    16921 taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc aggacgccaa
    16981 ccaccagttt gacgatggca atcaccagcg ccagcagcag cccaccgacg gtgaaggttt
    17041 ccatttgtcc ccacggaata aacaccccga cgaacatctg caacaccacc agctgtttca
    17101 ggctgatgcc ccatttcatg acgccaaagc cgctgccgct atactcagag agcggacctt
    17161 cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt tcgataaagg
    17221 tggcgaacgc acaggcacaa agcgccagca ccagcgggat gctctggctc agtggccagt
    17281 gataaacggt gtcggtgatg ttgctgatgt tggtggaacc ggcaacctgt gcggcgaccc
    17341 acagaccaag cagcaacatc ggttcaacca acacgccaag catcgcttca cggctcgcgc
    17401 cgatagcggt aaacgggcta ccagtatcca gaccagaaat ggcaaagaag aaacgcgcga
    17461 tggcgaagag atacagtaag gtgatcaaat cgccaagttg cggcagcgga gaaccgacgg
    17521 tcaccaccgg cagcgcagtg gcgatagtca gcatgacgcc caccatcaca tacggcgtca
    17581 ggcggaacac ccagccggag gcatccgggc caacgctctg gcggcccagc agtttgataa
    17641 tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt atgcagacgg gcacgcgcca
    17701 cacgggtaat accggagagc agcggcgcaa cggcaaatag caccagcgcc tgaattaacg
    17761 gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca gcaccgccag
    17821 ttcaaccagc gccatccggc ggaacagcaa cgcactcccc tcgcactgcc agcccggcac
    17881 cagcgacacc ggattcaacc atttgcgtag tttcagcacc ggcgcaaacg cctgtttcac
    17941 cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatgatcgt agccgcacac
    18001 ccaggccgcg ccgcgggaac gcgacggcaa acgatcgcct ttgcaaatcg ccataatgat
    18061 gaatggcagc agcgggcagg caatcagcag caacgtgatc atcggttgag aaacggtggt
    18121 gttagcaggc tccagcggca gaggtacagc agcagagagc atcggcagta gccacggcgc
    18181 agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc tcatcaggag
    18241 cggcgcacag gtggcgtttt cggcctcttt ggtacgcggc gcgccaagga aagtaacgcc
    18301 ataaactttc gccatacaca tcaccgccag cgcaccggta attgccagcc ccacggcgag
    18361 cagcggcccg agaagacggg caacaaacgc gccactattg ctcagtttga agaaggattg
    18421 atagataacc cattccccgg caaaaccgtt cagcggcggc agcgcagcca ttgccatcag
    18481 cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac cgagtttttc
    18541 gatatcgcga tgaccggtac ggaaccagac gctccccgca ccaaggaaca gtacgctctt
    18601 gaacaggcta tggttaagca gatggtacag accaccaacc agaccaaggg caatcagcgc
    18661 cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcagga taatgccgat
    18721 attctccagg gtgtggtaag ccagcagacg ttggatatta tgttccatca acgcatacag
    18781 accgccgacg aatgcggtga tcataccgag caccagcagc gcgatccccc accacagcgg
    18841 tgcattaccg cccagtagtg acagagttaa aatgcccagc aggccaattt tcatgactac
    18901 cgtggaaaac agcgcggcag ctggcgcaga ggcgttggca tgtgcctgtg gcacccagcc
    18961 gtgcagcgga ataatcccgg ccagcaggcc aaagccaatc acaccgagca gccagatatc
    19021 ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca gcgtgccgta
    19081 acgctgccac agcagccagc aggcaatcgc cagcagcaga gtgccaagac gccccagcgc
    19141 aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca gggccatgat
    19201 ttcggccatt accacgaaca tgccgaggtt gctggcgatg acggcacaga cggcggcagc
    19261 catcaacata ttgatctgca agccgttgca tttcacctgc gcgtggcgat gccagtcaat
    19321 gttgtagagg ctgacaaaca gaccgcatag gccgagtgta atcagccaaa tcgcattaag
    19381 cggagagatt tgcacatcgt agcttaccag cgacagcgca ccgctcacgc caaccgcgcc
    19441 agtcagcaca gtgaagcccg cggctgccgt atacaggcta cccaccgcgc cgccaattcc
    19501 ggctatccag ccacttagcg ctttttgaaa agaaaagaga aatgccagaa cagcggcggc
    19561 gacaaaccac gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    19621 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaagtcag ctcacgctta
    19681 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    19741 atccgcacgc aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    19801 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    19861 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    19921 tcaattgcgc caaacgggca ggcgatacca cacagtttgc agcttacgca cagactttca
    19981 ttcaactgca cagcaccatc aacacgagtg atggcgttaa ccgggcagac caccgcgcag
    20041 ggtgcatctt cacagtgatg acagagctgc ggcgcagatt ctttttcatt tagcatcact
    20101 cgcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcagcctca
    20161 caagtgtggc agccgataca gagcgtggag tcagcaatta caaaacgatt caccaggcat
    20221 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    20281 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    20341 tgctaccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    20401 ggcggtatat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacttg
    20461 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca actgcgcctg
    20521 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    20581 gttaaagcca tccgccaagg tgacgtaaat gcgaaaatgt tcaaaaagga cgaaacagag
    20641 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    20701 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    20761 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac ccgtttaaca
    20821 gagatgccag ctttaagcat tttctgtgcc aacttttaat tcattgttat taaagcgatt
    20881 tctaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    20941 attcatcgca gggataatca acactggcac aattattgct tgtagctggc aatagttaat
    21001 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    21061 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    21121 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    21181 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    21241 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    21301 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    21361 agcgatgtgt acaacatgcg gttgcggtga aggcaacctc tatatcgagg gtgatgaaca
    21421 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgaagatgaa
    21481 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    21541 ttacggtcat ggcgaagcgg gcactcacgc gccgggcatg agccagcgtc ggatgctgga
    21601 agtcgaaatt gacgtgctgg acaaaaataa ccgtctggct gaacgcaacc gcgcgcgctt
    21661 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    21721 cctgctgacg gaaactttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    21781 tgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggcaccc cagcgattca
    21841 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcgccgcg
    21901 cctgccactg gacgataacg gtattctgtt tatcgaaaac gtcggtaacc tcgtttgccc
    21961 ggccagcttc gatctcggtg aaaaacacaa agtggcggtg ctttccgtta ccgaaggtga
    22021 agacaaaccg ctgaaatatc cgcacatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    22081 agttgacttg ctgccgtatc tcaatttcga cgttgagaag tgcatcgcct gcgcccgtga
    22141 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    22201 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcact
    22261 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    22321 ttagtcggca gctgcgatga aaacggtcag ccacgcgtgg gccagtgggt actggttcac
    22381 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    22441 caaaacatgt ttgacgttga gccagatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    22501 tgcgttttgt tgatgaatat cgcgagccgg aacaggtaat gcagttaatt gagcatctgc
    22561 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    22621 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttgcta ccggaaaacg
    22681 ttgagtttat ccacggtccg ggttgccctg tgtgcgtact gccaatgggt agaatcgaca
    22741 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gatgccatgc
    22801 gcgtaccagg gaaacagggg tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    22861 tcgtttactc gccaatggat gcgttgaaac tggcgcagga gaatccaact cgtaaagtgg
    22921 tattcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    22981 cgaaagcccg cgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    23041 cactgcgcag tttgctggaa cagccagata acggtatcga cgcgttcctc gcgccgggcc
    23101 acgtcagtat ggttatcggc actgatgcct ataattttat cgccagcgct tttcatcgtc
    23161 cgctggtggt cgctggtttc gaaccccttg atctacttca aggcgtggtg atgctggtgg
    23221 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    23281 ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagtg
    23341 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    23401 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgtg
    23461 cgcgttgtgg cgaagtcttg acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    23521 acacctgtaa tcctcaaacc gcgtttggtg cgctgatggt ttcctccgaa ggagcgtgcg
    23581 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    23641 cggtagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    23701 caacccgtgg ctggcagagc aggaagatca ggcccgtctt gatctggcgc agctggtagc
    23761 ggaaggcgac cgtctggcgt tctcaaccga cagttacgtc attgacccgc tgttctttcc
    23821 tggcggtaat atcggcaagc tggcgatttg cggcaccgcc aatgacgttg cggtcagtgg
    23881 cgcaattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    23941 actgaaagcc gtagtgacca gcatggcaga aaccgcccgc gcggcaggca ttgccatcgt
    24001 tactggcgat accaaagtgg tacagcgcgg cgcggcagat aaactgttta tcaacaccgc
    24061 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    24121 tgtattgctg gttagcggta cactcggcga ccatggggcg actatcctta acctgcgtga
    24181 gcaactaggg ctggatggag aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    24241 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    24301 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaacttt cagaagcggc
    24361 actgccggtt aaacctgctg tgcgcggcgt ttgcgaactg ctgggactgg acgcccttaa
    24421 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcagcag agcaagtgct
    24481 ggcggcgtta cattcccatc cattgggaaa agatgcggcg ctgattggcg aagtagtaga
    24541 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atctaccgca
    24601 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    24661 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    24721 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    24781 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    24841 cggcgattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgca tcgcgtgaaa
    24901 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    24961 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    25021 agctggccac aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    25081 cggaagggca tatttttggt ggctgtgaat ttattcgtta tgacgatcgc ccctggagcg
    25141 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    25201 tccagagccg cgtcgttaac aatgtcgact atgagttgtt atgccgggaa cgcgataact
    25261 tccgcatcct ggtcgccatc actaacgcgg tgctttcccg cctggatatg gacgaactgg
    25321 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agcatcgtct
    25381 tacgcagcca ccgtaagaac aaactcaaca tctactccac tcactatctt gataaacagc
    25441 atcccgctca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtcttca
    25501 aaagtaaaga gatgctgttg attaatctcc acgagcggga cgatttagcc ccctatgaac
    25561 gcatgttgtt caacacctgg ggcaatcaga ttcaaacctt gtgcctgtta ccgctgatgt
    25621 ctggcgacac catgctgggc gtactgaaac tggcgcaatg cgaagagaaa gtctttacca
    25681 ctaccaatct gaatttactg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    25741 ccctcgccta tcaggaaatt catcgtctga aagaacggct ggttgatgaa aacctcgccc
    25801 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    25861 ccatgtacag cgtacttaaa caagtggaaa tggtggcgca aagtgacagt accgttctga
    25921 tcctcggtga aactggtaca ggtaaagagc tgattgcccg cgcgatccat aatctcagtg
    25981 ggcgtaataa tcgccgtatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    26041 aaagcgatct atttggtcat gagcgtgggg cttttaccgg tgccagcgct cagcgcattg
    26101 gtcgttttga actggcggat aaaagctcac tattcctcga tgaagtgggc gatatgccgc
    26161 tggagttaca gccgaagttg ctgcgtgtat tacaggaaca ggagtttgaa cgtctcggca
    26221 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    26281 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    26341 tccacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    26401 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    26461 gcatcctgag caacatggaa tggccgggca acgtgcgtga gctggaaaac gtcattgagc
    26521 gcgcggtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    26581 cagaacctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    26641 agttgattgt tcgcgtgctg aaagaaacaa acggcgtggt tgccgggcct aaaggcgctg
    26701 cgcaacgtct ggggctgaaa cgcacgactc tgctgtcacg gatgaagcga ctgggaattg
    26761 ataaatcggc attgatttaa cagatgtggt tatactcgat caacattcaa tgaaaggagg
    26821 cagtatggca gtttcagcaa gaaatcaatt aaccggaaca gttagcacag tagcaatggg
    26881 cgctgtgaat gatgaagtag aactgacgct ggcaggtggt gcaaagctgg tggcaattgt
    26941 gactcacagc agccagcaag cgctgggtct ggcgaaaggg aaagaagcga ttgccctgat
    27001 taaagccccg tgggtcacgc tggcaacgga agactgtgga ctgaaattct ctgctcgcaa
    27061 ccagtttgct ggcagcgtgt cgacaatcac cgaaggcgca gtgaacgcca ctgtccatat
    27121 caaaacggat gcgggttttg aaattgtggc agtggtaaca aatgaaagtc aggatgaaat
    27181 gaaactcacc actggctcgc gcattattgc attgatcaaa gcatcagcca tcctgattgc
    27241 caccaaagcg taagcgccgt atttgccgga gagtatggct ctccggcaga tttatttctt
    27301 tggacgatat ttttctggca actccggcac cggacgcttg tcatcgatga gatgacgcac
    27361 ggttaagatc ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat
    27421 ctcttcacgc tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc
    27481 ttcgccgaac acacatttat ccagccgctt ttgcgcgtaa gcaaacaacg cctcgtaatg
    27541 ctccggctct gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat
    27601 cgtcagtttt tcacgagaga tacgcttgcc ggacatgctg cctccatact cttaaggtgc
    27661 atttatatta caacttaatt ttaaagggga ctatgtctct gaaggcggag gtttcttcaa
    27721 ccgacgaatt tggcgatctt gtttcatcgt catcacgcaa aagctgtaaa gcagcatttt
    27781 tcgcggaacc gacatcaaga actcaccttt cgcgtcttcc cctgaaatga ttaactccgg
    27841 tatcatgtgc gccttatgtg attacaacga aaataaaaac catcacacta catttaacat
    27901 cagggagccg gacttaaccc catgagtaca atagaaaatt tcgacgccca tacgcccatg
    27961 atgcagcagt atctcaagct gaaagcccag catcccgaga tcctgctgtt ttaccggatg
    28021 ggcgactttt atgaactgtt ttatgacgac gcaaaacggg cgtcgcaact gctggatatt
    28081 tcactgacca aacgcggtgc ttcagcggga gagccaatcc cgatggcggg gattccctac
    28141 catgcggtgg aaaactacct cgccaaactg gtgaatcagg gcgaatcggt tgctatttgt
    28201 gaacagattg gcgatccggc gaccagcaaa gggccggtcg agcgcaaagt tgtccgtatc
    28261 gttacgccgg gcaccatcag cgatgaagca ctgttacagg agcgccagga caatctgctg
    28321 gcggctatct ggcaggacag taaagggttc ggctacgcaa cgctggatat cagttccggt
    28381 cgttttcgcc tgagcgaacc ggcggaccgc gaaacgatgg cggcagaact gcaacgtacg
    28441 aatccagcgg aattactgta tgcggaagat ttcgccgaga tgtcgctgat tgaaggtcgc
    28501 cgcggcctgc gtcgtcgccc gctgtgggag tttgaaatcg acaccgctcg ccagcaattg
    28561 aacctgcaat ttggcacccg agatctggtc ggttttggtg tggagaacgc accacgcgga
    28621 ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgcac gaccctgccg
    28681 catattcgtt ctatcactat ggaacgtcag caggacagca tcattatgga tgccgcgacg
    28741 cgtcgtaacc tggaaattac tcagaacctg gccggcggtg cggaaaatac gctggcttca
    28801 gtgctcgact gcactgtaac gccgatgggc agccgtatgc tgaaacgctg gctgcatatg
    28861 ccagtgcgcg atacccgcgt gttgcttgag cgccagcaaa ctattggcgc attgcaggat
    28921 ttcaccgctg agctacagcc ggtactgcgt caggtcggcg acctggaacg tattctggcg
    28981 cgtctggcgt tgcgtaccgc tcgcccgcgc gatctggccc gtatgcgtca tgccttccag
    29041 caacttccag aactgcgcgc gcaactggaa ggtgtcgata gcgccccggt acaagcactg
    29101 cgtgagaaga tgggcgagtt tgccgaactg cgcgacctgc tggagcgagc aatcatcgac
    29161 acgccgccgg tgctggtacg cgacggtggt gttattgcga ctggttataa cgaagaactg
    29221 gatgaatggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct ggaagtccgc
    29281 gagcgtgaac gtaccggcct ggacacgctg aaagttggct ttaatgcggt gcacggctac
    29341 tacattcaaa tcagccgtgg gcaaagccat ctggcaccaa tcaactacat gcgtcgccag
    29401 acgctgaaaa acgccgagcg ctacatcatt ccagagctaa aagagtacga agataaagtt
    29461 ctcacctcaa aaggcaaagc actggctctg gaaaaacaac tttatgaaga gctgttcgac
    29521 ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc ggaactcgac
    29581 gtgctggtga acctggcgga gcgggcctat accctgaact acacctgccc gacctttatt
    29641 gataaacctg gcattcgcat taccgaaggt cgccatccgg tagttgaaca agtactgaat
    29701 gagccgttta tcgctaaccc gctgaacctg tcgccgcagc atcgcatgtt gatcattacc
    29761 ggtccgaaca tgggaggtaa aagtacctat atgcgccaga ccgcgttgat tgcgctgatg
    29821 gcgtatatcg gcagctacgt accggcgcaa aaagtcgaga ttggcccgat tgaccgtatc
    29881 tttacccgcg taggtgccgc ggatgatctg gcttccgggc gttcaacctt tatggtggag
    29941 atgaccgaaa ctgccaatat tttacataac gccaccgaat atagtctggt gttgatggat
    30001 gagattgggc gtggaacgtc cacttacgat ggtctgtcgc tggcgtgggc gtgtgcggaa
    30061 aatctggcaa ataagatcaa agcgttgacg ctgtttgcta cccactattt cgagctgacc
    30121 cagttaccgg agaaaatgga aggcgtcgcc aacgtgcatc tcgatgcact ggagcacggc
    30181 gacaccattg cctttatgca tagcgtgcag gatggcgcag cgagcaaaag ctacggcctg
    30241 gcggttgcag cgctggcagg cgtgccaaaa gaggttatta agcgcgcacg gcaaaaactg
    30301 cgtgagctgg aaagcatttc gccgaacgcc gctgctacgc aagtggatgg tacacaaatg
    30361 tctttgctgt ccgtaccgga agaaacttcg cctgcggtcg aggcactgga aaacctcgac
    30421 ccggattcac tgactccgcg tcaggcgctg gagtggattt atcgcttgaa gagtctggtg
    30481 taataataat tcccgatagc cttttgctat cgggaatatt aacgacaact gacgaatcaa
    30541 ataaaaatac cctgtataat aggaaaactt attttacagg gtaaaaccat gccatctaca
    30601 cgctaccaaa aaatcaacgc ccatcactat cgccatatat ggaccgttgg tgatattcat
    30661 ggtgactatc aattattaca atcccgctta catcaactct ctttttgccc tgaaaccgac
    30721 ttacttattt cggtaggcga taacatcgat cgtgggcccg agagtcttaa cgtgctgcgc
    30781 ctgttaaacc agccctggtt tatctccgtc aaaggcaacc acgaagcgat ggcgcttgat
    30841 gcattcgaaa ctggcgatgg aaatatgtgg cttgccagcg gtggtgactg gtttttcgat
    30901 ttaaatgatt cagagcaaca agaggcaata gatctgttgc tgaaattcca tcaccttcca
    30961 catattattg aaatcactaa cgacaccata aaatatgtca tcgcacatgc agattatccg
    31021 gggaaggagt atcagtttgg caaagaaata gcggagaatg aattactctg gcctgttgat
    31081 cgtgtgcaga aatcacttaa tagcgaatta caaaaaataa ac
//
LOCUS       DAZFRG010000028        30805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFRG010000028 DAZFRG010000000
VERSION     DAZFRG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.028"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..587
                     /locus_tag="ACHKUC_001009"
     CDS             6..587
                     /locus_tag="ACHKUC_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO6617300.1"
                     /translation="MIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRADLIPNLV
                     ASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQTWQQAQAQVSRTLGQL
                     TIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAK
                     VMDYTPKKNYLPDDVAAVSKAPTIDFSQNTNAH"
     gene            601..1272
                     /locus_tag="ACHKUC_001010"
                     /pseudo
     CDS             601..1272
                     /locus_tag="ACHKUC_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1524..2672
                     /locus_tag="ACHKUC_001011"
     CDS             1524..2672
                     /locus_tag="ACHKUC_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO6617301.1"
                     /translation="MRIFLLLLMLYGFQALAEQLPSVTDLTGTLTTEEQTALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQSYGLYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLKEMLPPEQAAQIVHYHMQPEFKKNNLFSGLTQGIEGIAQFTYIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFTSRPGPGIWMISMITPMVWFFFFQ
                     DDVIIRRVSLVCFSLFFAATCWQRIAVIFHMYRVFPMMLAPKNKNAKVKKQKTTQQVS
                     QSERNSLFVVLLIIVVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGHKSNRKTGNRNAHSRSSSKNSFRGGGGSSGGGGSSDRW"
     gene            2678..3550
                     /locus_tag="ACHKUC_001012"
     CDS             2678..3550
                     /locus_tag="ACHKUC_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6617302.1"
                     /translation="MRIILFLLTLWCLPVLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITQKTRAQVAVLIVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(3610..4908)
                     /gene="eno"
                     /locus_tag="ACHKUC_001013"
     CDS             complement(3610..4908)
                     /gene="eno"
                     /locus_tag="ACHKUC_001013"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO6617303.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4996..6633)
                     /gene="pyrG"
                     /locus_tag="ACHKUC_001014"
     CDS             complement(4996..6633)
                     /gene="pyrG"
                     /locus_tag="ACHKUC_001014"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO6617304.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6861..7652)
                     /gene="mazG"
                     /locus_tag="ACHKUC_001015"
     CDS             complement(6861..7652)
                     /gene="mazG"
                     /locus_tag="ACHKUC_001015"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO6617305.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7724..8059)
                     /gene="mazF"
                     /locus_tag="ACHKUC_001016"
     CDS             complement(7724..8059)
                     /gene="mazF"
                     /locus_tag="ACHKUC_001016"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO6617306.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8059..8307)
                     /gene="mazE"
                     /locus_tag="ACHKUC_001017"
     CDS             complement(8059..8307)
                     /gene="mazE"
                     /locus_tag="ACHKUC_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO6617307.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8385..10619)
                     /gene="relA"
                     /locus_tag="ACHKUC_001018"
     CDS             complement(8385..10619)
                     /gene="relA"
                     /locus_tag="ACHKUC_001018"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO6617308.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRDSVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10667..11968)
                     /gene="rlmD"
                     /locus_tag="ACHKUC_001019"
     CDS             complement(10667..11968)
                     /gene="rlmD"
                     /locus_tag="ACHKUC_001019"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO6617309.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12025..14781
                     /gene="barA"
                     /locus_tag="ACHKUC_001020"
     CDS             12025..14781
                     /gene="barA"
                     /locus_tag="ACHKUC_001020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO6617310.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRNGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(15012..16352)
                     /gene="gudD"
                     /locus_tag="ACHKUC_001021"
                     /pseudo
     CDS             complement(15012..16352)
                     /gene="gudD"
                     /locus_tag="ACHKUC_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
     gene            complement(16373..17713)
                     /gene="gudX"
                     /locus_tag="ACHKUC_001022"
     CDS             complement(16373..17713)
                     /gene="gudX"
                     /locus_tag="ACHKUC_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO6617311.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQAHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17715..19067)
                     /gene="gudP"
                     /locus_tag="ACHKUC_001023"
     CDS             complement(17715..19067)
                     /gene="gudP"
                     /locus_tag="ACHKUC_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO6617312.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19501..19950)
                     /gene="yqcA"
                     /locus_tag="ACHKUC_001024"
     CDS             complement(19501..19950)
                     /gene="yqcA"
                     /locus_tag="ACHKUC_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO6617313.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(19968..20750)
                     /gene="truC"
                     /locus_tag="ACHKUC_001025"
     CDS             complement(19968..20750)
                     /gene="truC"
                     /locus_tag="ACHKUC_001025"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO6617314.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWCGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20750..21079)
                     /gene="yqcC"
                     /locus_tag="ACHKUC_001026"
     CDS             complement(20750..21079)
                     /gene="yqcC"
                     /locus_tag="ACHKUC_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO6617315.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDYPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21701..22246)
                     /gene="syd"
                     /locus_tag="ACHKUC_001027"
     CDS             complement(21701..22246)
                     /gene="syd"
                     /locus_tag="ACHKUC_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO6617316.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            22314..23162
                     /gene="queF"
                     /locus_tag="ACHKUC_001028"
     CDS             22314..23162
                     /gene="queF"
                     /locus_tag="ACHKUC_001028"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO6617317.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNNDFVPST
                     TRLVRQ"
     gene            23274..24638
                     /gene="ppnN"
                     /locus_tag="ACHKUC_001029"
     CDS             23274..24638
                     /gene="ppnN"
                     /locus_tag="ACHKUC_001029"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO6617318.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEKFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            25195..26484
                     /gene="sdaC"
                     /locus_tag="ACHKUC_001030"
     CDS             25195..26484
                     /gene="sdaC"
                     /locus_tag="ACHKUC_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO6617319.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            26542..27909
                     /gene="sdaB"
                     /locus_tag="ACHKUC_001031"
     CDS             26542..27909
                     /gene="sdaB"
                     /locus_tag="ACHKUC_001031"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO6617320.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            28021..28776
                     /gene="xni"
                     /locus_tag="ACHKUC_001032"
     CDS             28021..28776
                     /gene="xni"
                     /locus_tag="ACHKUC_001032"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO6617321.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(28880..30028)
                     /gene="fucO"
                     /locus_tag="ACHKUC_001033"
     CDS             complement(28880..30028)
                     /gene="fucO"
                     /locus_tag="ACHKUC_001033"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6617322.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDVAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(30056..30703)
                     /gene="fucA"
                     /locus_tag="ACHKUC_001034"
     CDS             complement(30056..30703)
                     /gene="fucA"
                     /locus_tag="ACHKUC_001034"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6617323.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         7547 a         8133 c         7359 g         7766 t
ORIGIN      
        1 tcgcaattat atttattttc aatttatcag gctgtggata taacgatatt caaacctatg
       61 atgagcaggt taacgcctcc tggtctgagg tattgaatca atatcaacgt cgcgccgatc
      121 ttattcctaa tcttgtcgcc agcattaaag gttattccag ccatgaacga gatgtactgg
      181 aagccgtaac attggctcgt agccaggcta atcgcgccag tagcgatctt cagcaaactc
      241 cgggagatga acaaaaatta caaacctggc aacaagctca ggcgcaggtg tctcgcactc
      301 tcggtcagtt aacgataatt agtgaacgtt accctgagct aaaggcccag gaactctatc
      361 aaaatttgat ggtacagctc gaaggaagtg aaaatcgaat tgccgttgcg cgaggaagat
      421 atattaaagc tattgaacaa tataatgtca ctatccgcaa attccccgca gtgttaaccg
      481 caaaggtcat ggattacaca ccaaagaaaa attatttgcc ggatgatgtc gccgctgtga
      541 gtaaagcccc gacaatagat tttagccaga atactaatgc tcattaagcg gggaagtcct
      601 gtgcgaattt ttatcttcct tatggctttc ttctgttcat ctgtgtttgc cacacaaatt
      661 ccagtgcctg aatcacccag gtatgtgaat gacttaaccg gtaaattgac gaacgatgaa
      721 gtaaatactt tgaccaatca gattaaagag ctgacgcaaa aaagccacgc acaactggtc
      781 gtgttagtgg ttgaaacaac gggcgatgaa accatcgagc agtacgctac gcgggtattc
      841 gatagctgga aaccaggtga taaagaccgc gatgatggcg ttctactgtt agtcgcctgg
      901 caggatcata ccgtgcgtat tgaaatcggt tacggactgg aaggcgttat tacggatgcg
      961 cagtccggga aaattattcg caacagtatt attccggcat ttaaaaatgg cgacctggct
     1021 ggcggcttgc aaaagggaat aagcgatatt gataatcgtc tgacggggaa taatttagca
     1081 acgataacac cgcccgatca ccctttgccc tttaacggtt ggtgggcttt gttagtctgg
     1141 gcaatagtac tgacctttat ttccgccaga ggatatttca aaacgctggg actaatttgc
     1201 ttcacagcta ttgtactggc ctttgtttta cctatagcag gtttcagcgg aagctgggga
     1261 gtactcgcaa cattactctg cttcgctaca ccgttcctgg ctgtcgctat cgcttttacc
     1321 cccttcggca aaaaggttcg ggattcgatg cgtagtgcca accgaccgtc acgttacgaa
     1381 agaagtaact cggcctcctg gcgttcttca tcatcgtcgt catcttcctc ttcatcctct
     1441 tttgacaatg ataatttcag cggcggcggc ggttcatctg gcggcggtgg cgcctccggg
     1501 cgctggtaac aaaaggaatt gaaatgcgta tttttctctt acttctgatg ctatatggtt
     1561 tccaggcgtt agctgaacaa ttgccttcag taactgactt aaccgggaca ttaacgacag
     1621 aagaacaaac tgctttaaat caacaattac agactctcga gcagcaaaac cacttccagg
     1681 tggccgtgtt ggttacgccg accacagggg gcaaaaacat taaacttgca gcctctcaga
     1741 gctacgggtt atatcactgg aaacccgttg gagagaagcg gtacggcgaa ggtatcttaa
     1801 ttctcgtcgt ttggccggaa gggctggcgt caatgaagat tgggcatgga ctgaaagaga
     1861 tgctgccacc ggaacaagct gcgcaaattg ttcattacca tatgcaaccg gaattcaaaa
     1921 aaaataatct cttctccggg ttaacgcagg gaattgaagg catcgcacag tttacctata
     1981 taaaagcgaa tcttagccct ttggatgctt tagccaatca tctgtttgct aatccgcaac
     2041 gctcgctgcc atgtcttgcc tggacattac tgatgatcgt tgccatcgtt gtcctctggc
     2101 gatttaccag ccgtcccggg cctggcatat ggatgatatc gatgattact cctatggtct
     2161 ggtttttctt ctttcaggat gatgtcatca ttcggcgggt atcgctcgtt tgttttagtt
     2221 tatttttcgc agcaacctgc tggcagcgta tcgcggtaat ttttcatatg tatcgcgtat
     2281 ttcctatgat gctggcccca aaaaataaaa acgctaaagt gaaaaaacaa aagacgactc
     2341 agcaggtttc tcaatccgaa cgaaactcat tgtttgtcgt gctgctgata attgttgtgg
     2401 gatggtgcct ggtattcgcc agtaataaag atatagcatt catctcaaca attattgggc
     2461 taattgatca ttttatcggc ccaataacca ttgctggcat cgttctgctg atcattgtgg
     2521 ccaaatttac tggcaatctt aaactgggat caggacataa aagcaacagg aaaacgggga
     2581 atcgtaatgc tcattctcgc tcttcgtcga aaaattcttt ccgcggtggc ggcggttctt
     2641 caggaggcgg cggttcatcc gaccgttggt gatcgttatg cgtataatcc tttttctact
     2701 cactttatgg tgccttcctg tgcttgccca gcaaattgct gtgccggagc tgcgccaaca
     2761 ggtgaccgat attactggta ccttaagcac gtctgaacaa caatcactaa cacaacagct
     2821 gcaggatatt acacaaaaaa cacgggcaca ggtcgctgta ttaatagtgc catccaccgg
     2881 agacgattct attgaacaat atgctacaag agtttttgac aattggcgtt taggagatgc
     2941 caaacgcaat gatgggatac tgatcattgt tgcctggtcg gatcgcactg tccgcatcca
     3001 ggtaggttat gggctggaag aaaaggtaac cgacgctctg gctggagata tcatccgtag
     3061 caacatgata cccgcattta aacaacaaaa attagctaag ggattagagt tagctataat
     3121 cgctttgaat aaccgactca cttcacaaca ccaatattcg gttaatcctt cagaaagtga
     3181 atcagcatct tccagcgatc attactactt tgctattttt tgggtatttg cagtgatgtt
     3241 cttccctttc tggttttttc atcaaggcag taatttttgt cgcgcatgta aaagcagtgt
     3301 ctgtatttca gcaatctatc ttttagattt attcctgttc tccgataaaa ctttttccat
     3361 tgctgtattt ttcttctttt ttactttcac caccattatg gtctttacct gtttatgtgt
     3421 acttcagaaa aaagcatctg gtagaagtta tcattcagac aacagcggtt ccggtggtgg
     3481 ctcaagtggc ggcggattta gcggcggggg cggttcttct ggcggtggcg gcgcgtccgg
     3541 tcgctggtaa ctcaaccata aaaaatgcca gcccgaaggc tggcattttt aaatcagata
     3601 aagtcagact tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc
     3661 cagagcttct tcgatacgaa tcagctggtt gtatttagca acacggtcag aacggctcat
     3721 agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga tggtagcgtc
     3781 ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcgtctt tcgccatctt
     3841 gattgcagcc agagtttcag tcagagaacc gatctggttg aatttgatca ggatggagtt
     3901 agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc
     3961 accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc
     4021 agattcgtcc agaccgtctt cgatggaaac gatcggatac tgtttggtca gttcttccag
     4081 gaagtgagtg aattcttcag aggtgaacgc tttgttgcct tcgccagcca gaacgtattt
     4141 accgtctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt ctttgcccag
     4201 ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc
     4261 caggttcggc gcatagccac cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag
     4321 aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt
     4381 cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc
     4441 accgttgatg atgttcatca tcggaaccgg catagagtat ttgcccggag tgccgttcag
     4501 ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt tggcgttagc
     4561 cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag
     4621 gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg
     4681 agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga
     4741 tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc
     4801 agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc
     4861 acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc
     4921 aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta cgcgttgccg
     4981 ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa
     5041 gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca
     5101 agccacgaac cacggatgat tcggaacttc gatgatctcg accaactgat catccccgga
     5161 acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc
     5221 gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc gaaccaggct
     5281 atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt
     5341 ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac
     5401 cgggtactta cagtctggca caaattccgt agagttggcg ttctccatgt tggcaacatg
     5461 gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt
     5521 gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccgaaacc
     5581 gccaggtacg aggattgcgt ccagaccttt gaggatttca acgccgcgcg tttcaacatc
     5641 ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag
     5701 tgcttcgatc acggatttgt atgcatccgg cagctcaatg tatttgccga ccataccaat
     5761 ggtgacttca cttaccggat tcgcttcttc gaagataacc tgttcccatt cggacagatt
     5821 cgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt
     5881 caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc
     5941 cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacgg cgcgatctga
     6001 acgacaaatc aggatatcag gctggatacc gattgaaagc agctctttta cagagtgctg
     6061 agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat
     6121 aaacagggtg tgctcacggc caatttcaac agccatctgg cgaatcgctt cgaggaacgg
     6181 cagggattcg atatcaccta cagtaccgcc gatttcgacc agtactacgt catgaccttc
     6241 gccgccttcc agcacgcgct ctttgatagc gttagtgatg tgcggaataa cctgtacggt
     6301 tgcgccgaga tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgccccgt
     6361 ggtgaagttg ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt gccccaggtc
     6421 caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat
     6481 agtaccggga tcgacgttga tgtacggatc cagtttcatg atggtcacat tgaggccacg
     6541 ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac
     6601 cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa
     6661 gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt gccacaaact
     6721 ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct
     6781 ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatcg
     6841 cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact
     6901 tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc
     6961 tccacttcgc gaaaacgacg ctcgaatttt tcgttcgctt tttgcaatgc gatttccgct
     7021 tttgtcccta aatgacgagc cagattgacc gtggcaaaca gcaggtcacc catttcctcc
     7081 tccagtttag cctggtcgac aacagcctgc cgcgcctcgt acatcacctc gtcgatctct
     7141 tcgtagactt tatcgactac cggaccaagc gtcgtccagt cgaaaccaac gttggcgcaa
     7201 cgtttctgaa ttttttgcgc acgcattaaa gccggtaaac tacgaggaat atcgtccagc
     7261 gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc
     7321 acttcactac tattttcggc agaactgtca gcaaaaacat gcggatggcg acgctctaat
     7381 ttatcgctaa tagcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt
     7441 tgtgcgtaaa acaccacctg gaacagcaga tcgcccagtt caccgcgcag atcgtcaaaa
     7501 tcttcacggg cgatggcgtc cagcacctcg taggtttctt caagggtgta aggcgcaatg
     7561 gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccgggtcgcg caggcgctgc
     7621 atgatggtga gcaaacggtc gatttgattc attgaattat cctgaaaatt gcgggtccgt
     7681 caggtggaaa cctgtgacca gaatagaagt gagttagtaa cacctaccca atcagcacgt
     7741 taattttggc tttaatgagt tgtaattcct ctggggcaac tgttcctttc ttcgttgctc
     7801 ctcttgcccg ccaggcgatt ctttttacct gatcagctaa cgctacgcca tcacgttcct
     7861 gaccggatag aacaacttcg aacggatatc cttttgattg cgttgtacaa ggaacacaca
     7921 gacacatacc tgttttgttg ttgtacatga acggactcag gacaacagcc ggacgatgtc
     7981 cggcttgctc gctacctttt gtcgggtcaa aatcaaccca aatcagatcg cccgtatcgg
     8041 gtacgtatcg gcttaccatt accagacttc cttatctttc ggctcccccc agtcgatatt
     8101 ctcgtggagg ttttccggcg tgatgtcgtt gaccagttca gcaagcgtaa atacgggctc
     8161 tttacgcact ggctcaataa ttaatttgcc atccaccagg tcaatcttca cttcatcatc
     8221 aatattcaga ttgagcgcct gcattaacgt agccgggatc cgcaccgccg gtgaatttcc
     8281 ccaacgcttt acgctactgt ggatcataac cctttcctca aaccgcgatc atatgtagat
     8341 acagtatata tcaatctaca ttgtagatac gaacaagttt cggcctaact cccgtgcaac
     8401 cgacgcgcgt cgataacatc cggtacctgg ttgagtttac ccagcacgcg acccagcact
     8461 tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta
     8521 cggctggcaa cgccaagtac gttcaccttc tcgttggcga gaatggtcgt gatatcacgt
     8581 aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc ggcggagtag
     8641 ctctcgcccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag ttccgccaat
     8701 tgttcgcaat cggcgcggtg tactgaaata ccgcgccctt gggtaatgaa gccgacaatc
     8761 tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt accgacccct
     8821 tctaccacca cacgaccgtt atctttactg cggttttgcg gcgtgtagct tttttgctga
     8881 agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa ttgcgattgc
     8941 aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc cagcaactcg
     9001 tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt caggctgatc
     9061 cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg
     9121 tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt gacgtaaccg
     9181 aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat aatttcaatc
     9241 tggtcgccca tctgcagctg gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg
     9301 atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg cgttgatccc
     9361 gcaggcaaat caacgacatc acctttcggc gtaaagacgt acactcggtc gtcaaagacc
     9421 tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg ccacgcaatc
     9481 agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc agcagccgcg
     9541 ccctctttat atttccagtg cgcagcaaca cccaactctg catcttcatg catctgtttg
     9601 gtgcggattt ggatctcaac ggttttgcca cccggcccca gaaccacggt atgaatggac
     9661 tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg caggtggcga
     9721 tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc ggcgacaata
     9781 cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg cattttgcgc
     9841 cagatactgt agatgtgttt cggacggcca tacacttcag ctttaacgcc ttcagctttc
     9901 atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc gagacggcgt
     9961 tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa
    10021 tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg cgcgtagata
    10081 ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt tacttcgcgc
    10141 agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc gaccatcgcc
    10201 aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct
    10261 ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac gaccgactta
    10321 ccgacgctat cacgcagcac atcttcgctg actacgttgg catcggccag agggaacagc
    10381 agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc caccatctca
    10441 acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg cagacaatac
    10501 gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag acttgcgatc
    10561 catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac cgcaaccatc
    10621 gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat ttaacgcgcg
    10681 agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc atcgccagtc
    10741 gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc gttgcagggt
    10801 tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc ataacacctg
    10861 cggcacctgc tcgcgccggg tccaacaaca ctttatcgaa gccgtttttc gcccacggct
    10921 gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt aagccattaa
    10981 gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca cccaccacac
    11041 tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac agatccagta
    11101 cgcgatcttc aggttccaca tccagccatt ccaacgcacg cgctaccatt ttctggttca
    11161 cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac ccatttgagt
    11221 cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg gcgagataca
    11281 gatccaggcc ttcagaatgc gaaaagcatt ccagtttttc gcgatctgcc gaacttagcg
    11341 gtgcggtatg gcgcaaaatc attagcgtgc cgctggttgc ctgtaccagt tcaacatgac
    11401 caagatggcg catagcttgt aagctgccca ggcatgccct gactttgggc agcaatgctt
    11461 caagttgggg cactaaaatg gggcattgtt tcacgtcgac aatgtcactg gagcccgctt
    11521 tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc
    11581 gacggcgata gccccaggga acatcggcga tcacttcaga gacttcgtgt ttcattaatc
    11641 gggcgagtgc cgcacttttg cttcgctgct gtaaatccac actggcgtgt tgttgctgac
    11701 agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg ctatcgctta
    11761 accgacgtac gactttagcg cgggcatact gttttttatc ttcagtaaca gtaacttccg
    11821 cgttttcctg cggcaataat ccggggataa ataatgtttt gccgttatgt cgcgccacac
    11881 cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc
    11941 gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc
    12001 ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg catgatgatt
    12061 ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt cgtcgtgcat
    12121 cgctataacg acttgcagcg tcaactggaa gatgccggtg ccagtattat tgagccgctt
    12181 gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg tcagttaata
    12241 agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta tgatgaaaat
    12301 aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca gctcggcagc
    12361 aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat gatcctccgc
    12421 acgccgatta tttctgaaag ttactccccc gacgaatcgc ccagtagcga tgccaaaaat
    12481 agtcaaaata tgctgggata tattgcgctg gagctggatc ttaaatcggt tcgcttgcag
    12541 caatataaag agatctttat ttccagcgtg atgatgctgt tttgtatcgg tattgcgctt
    12601 atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat ggtgaatacc
    12661 gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat gctcggcgag
    12721 ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggcggc ttatcacgaa
    12781 gagatgcaac acaatatcga ccaggcgacg tccgatctgc gtgagacgct ggagcaaatg
    12841 gagattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt
    12901 aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa tggtgttatt
    12961 ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat
    13021 acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct cgacttctcg
    13081 aaactggaag ccggtaagct gattctggaa agtattccat tcccactacg cagcacgctg
    13141 gatgaagtcg ttaccctgct ggcacattct tctcacgata aagggctgga actgacgctc
    13201 aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactacgatt acagcaaatc
    13261 atcactaacc tggtagggaa tgcaattaaa ttcaccgaga atggcaacat tgatattctg
    13321 gtagaaaaac gtgcgctgag taataccaaa gtgcaaattg aagtgcagat tcgggatacc
    13381 ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttcag acaggctgat
    13441 gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac acaaaaactg
    13501 gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg ttcaactttc
    13561 tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc atccacccag
    13621 tgcctcgctg gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc acaatgcacg
    13681 ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt ctccgcgctg
    13741 cctcccgcgc attacgacat gatgttatta ggcatagcgg tgaccttccg cgagccgcta
    13801 acaatgcaac atgagcgatt agcgaaagca gtatcgatga ccgatttcct gatgctggca
    13861 cttccttgcc atgcacaagt caatgctgaa aaacttaagc aagatggtat cggcgcgtgt
    13921 ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac
    13981 aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg
    14041 gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga tatggtgcaa
    14101 catgtggaac tttgcgatag cgggcatcag gcggttgaac gggcgaaaca gatgccgttc
    14161 gatttaatct taatggatat tcaaatgccg gacatggacg gcattcgggc atgtgagctc
    14221 atccaccagc tcccgcatca gcagcaaacg ccggttatcg cggtaacggc gcatgcgatg
    14281 gccgggcaaa aagagaaact gcttggcgcg gggatgagtg attatctggc caaaccgatc
    14341 gaagaagagc gattgcataa tttgctgttg cgctacaagc ctggcagcgg tatttcctct
    14401 cgcgtcgtga cgcccgaagt caacgaaatt gtggtcaacc cgaatgcgac cctcgactgg
    14461 caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat gctgcaaatg
    14521 ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt tggagaaaac
    14581 ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta tagcggtgta
    14641 ccgcgtatga agaatctctg ccaactgatc gaacaacagc tacgtaacgg tactaaagaa
    14701 gaagatttag agccggagct gctggaactg ttggacgaga tggataatgt agcgcgcgaa
    14761 gccagcaaaa ttctcgggta atggacgtct tgtcgagtag caaccggatt tttatgacgc
    14821 tggtttcaga ttggtgacaa gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc
    14881 cggcctgcaa aaccaattaa attcaatgaa ttaaaaaatg atgtagccct gatacgtagc
    14941 gcaacaggca attttgcgtt tggcatcagt ctcaaaccgg ctccagatag agccggtttt
    15001 ggttttctgt cttaacgcac catgcacgga cgcttgttat cgaacgtcca gccggggatc
    15061 aggtactgca ttcccatcgc atcgtcacgc gcgccaagcc cgtgtttctg atacagctcg
    15121 tgcgctttca tcacttgatc catatcgatt tctacaccca gccccggttt ttctggcacc
    15181 tgaaccagac cgcctttgat ctctaacggt tctttggtca ggcgctgatt accttcctac
    15241 cagatccagt gcgtatcaat cgcggtgatt ttacccggtg cggcagcggc aacatgggta
    15301 aacatcgcca gggaaatatc gaagtggttg ttggagtgtg aaccccaggt caggccaaat
    15361 tcatgacaca tttgcgccac acgtaccgaa ccttgcatcg tccagaaatg cggatccgca
    15421 agcgggatat caacggattg caacgagagc gtatggccca tctgccgcca gtcggtggcg
    15481 atcatattgg ttgcggtcgg caggccagtt gcgcgacgga actctgccat cacttcacgc
    15541 ccggagaaac cttgctccgc accacacgga tcttctgcat aagccagcga acctttcagg
    15601 tatgtaccga ttttaatcgc ttcgttcagc gaccaggcac cgttaggatc gagcgtaata
    15661 cgcgcctgcg ggaaacgttg cgccagtgcc acaatagatt cggcttcttc ttccccggcc
    15721 agtacaccgc ctttcagttt gaaatcgttg aagccatatt tttcatacgc cgcttccgcc
    15781 aggcgcacca ccgcatccgg cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat
    15841 gagtcatccg gctggctttg atacggcagc ggtgtagctt tgcgattacc gacgaagaac
    15901 agataaccga gcatttcgac ttcgctacgc tgttgaccat cgcccagcag cgatgccacg
    15961 tttaccccca gatgctgccc cagcagatcc agcattgccg cttctatccc ggtaactaca
    16021 tgaatagtgg tacgcaaatc aaatgtctgc aaaccgcgcc caccggcatc acgatcggca
    16081 aaagtattac gcaccagcgt cagaacgttt ttgtattcac ccagcgtttt acccaccacc
    16141 agcggaatcg catcttccag cgttttacgg attttctcgc cgccgggaat ttcccctacg
    16201 ccagtgtgac cggaattatc tttgataatc acaatattac gcgtgaagaa cggtgcatgt
    16261 gcaccactca gattcatcag catactgtca tgacccgcca ccgggatgac ctgcatttca
    16321 gtaacaacag gcgtcgtaaa ttgagaactc atatttatgt cctttttcag aatcaatgac
    16381 ggccgaaaac gggacgttta cggtcaaagg tccagccggg gatcaggtac tgcatcgggc
    16441 ctgcgtcgtt acgcgcaccg ccaggcagac gtttataggc ctcatgtgcc ttttgtacct
    16501 gttcccagtc cagttccacg cccagaccgg gcgcatcagg aacggcaatt tttccgtttt
    16561 taatctccag cggatttttg gtcagacgac aatcgccctc ctgccaaatc cagtgggtat
    16621 cgatagcggt aggattaccc ggtgccgccg cgcccacatg ggtaaacatc gccagagaga
    16681 tatcaaagtg gttattagaa tggcagcccc aggtcagccc ccagtcgtcg caaagttgcg
    16741 ccacgcggac tgcaccggaa agcgtccaga agtgcggatc ggcaagtgga atatctaccg
    16801 cattgagcat caccgcatga cccatttcgc gccagttggt ggcgatcatg ttggtcgcga
    16861 cgggcaagcc ggtcgcccgt cgaaactccg ccatcacttc acgaccggag aaaccctgct
    16921 cagcgccgca cgggtcttcc gcataggtaa ggacatcatt cagccctttg cacagagaaa
    16981 tggcttcatc aagcagccag gcaccattgg gatcaacggt aatccgcgca tcagggaagc
    17041 gtttcttcaa tgcacgaaca gtgtcgattt cttgctcgcc aggtaacacg ccacccttaa
    17101 gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc cgccagacgc acaacggctt
    17161 cgctgttcat cgctttctga tgccgcaact gataccactc atggttgccc ggcgtatttt
    17221 ccagataagg aagatcggtt ttggtccgat caccgatata aaacagataa ccgagaacgg
    17281 taatagcatc acgttgcttg cctggcccta acagttcgca gaccggaaca ttcagcgcct
    17341 gacctaacag gtcaagcaag gcggcttcca gcgccgccac ggcattaacg cgcaattcaa
    17401 aagtccaggc acctttgccg aaggtatcaa aatcggctgc ctgattacct ttatgcgcct
    17461 gctgaaccac tttattcagg cgcgcaactt cctggcccag caccatcgga atagccttga
    17521 ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc aatgccggta tgcccggcat
    17581 tatcggtgag taccacaata ttgcgggtga aatatgcgtt atgtgcacca ccaatattaa
    17641 gcaacatgct gtcatgtccg gcgaccggaa tgactttcat atcagtaata acaggactgg
    17701 attgtgtcgt catcttattg ccccgcgaca ggtttcagct caatacgctt gatatcgccc
    17761 accagcacca ggtagctgag taccgcgatt aaggcatgaa caccaacata aatcagcgcc
    17821 ccattaaagg agccagtcgt gccaacgata taaccaattg cgattggcgt gacgatgcca
    17881 gaaatgttac caaacatgtt aaacaggcca ccggaaagac cgctgatctc ttttggcgca
    17941 gtatcagcca ttacagccca acccagcgca ccgatcccct taccgaagaa ggccagcgcc
    18001 ataaagccga tgatcatcca ctcaacgttg acgtagttgc agaacaccat caccatcgac
    18061 agcaacatgc ccatcacgat cggtgtttta cgcgcaatgt tcagtgaacc cgtgcgacgc
    18121 atcagccagt cggaaataat cccacccagc acaccgccga taaaaccgca aaccgccgga
    18181 acggaagcca caaagcccgc tttcagaatc gacatcccgc gcgcctgcac cagataaacc
    18241 gggaaccagg taataaaaaa gtaagtcagg gcgttgatac agtactgacc gatataaacg
    18301 ccgatcatca tccgtgaccc gagcagctgt ttgatctgcc cccacttcac gctgaacggc
    18361 actttaactt tggtgttttg ctgatccata ttgatcagcg cgccacccgc ggcgatgtac
    18421 tccagctctt tctgatttac gcccggatgt tgatttggct cgtggatgac tttcaaccag
    18481 ataaagctga tgacaatgcc cagaccgccc ataaagaaga agacgtgtga ccagcccact
    18541 tcatgagtca accagcccat aatcggcgcg aagataaccg ttgcgaagta ttgggcggag
    18601 ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa accaggccgc aacaatgcgg
    18661 ctgttgccag ggaaagaggg cgcttcagca agcccgacca ggaagcgcag tgtaaacagg
    18721 gcaacgataa tgccgaatcc actaaagata tcgacgaagc cttgcagcaa ggtaaacatc
    18781 gaccagataa agatcgacca gaagtagacg cgttttgaac caaaacgatc cagcaaccag
    18841 ccaccaggga tctgcccgat aacatacgcc catgagaagg cagagaacac atagcccatt
    18901 cccacgggat caaggccgat atctttggcc atttccgaac cggcaataga aagcgtagcg
    18961 cggtcgccgt agttgaagga tgtgacgata aacaacatca ccactatcca gtaacgagca
    19021 ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag aactcattgt tgcactcctg
    19081 aaaattcgcg ttagccacgc tcactctgga ctgcgacatc gccaggaaat cagaggtgac
    19141 gtagggtgtt tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg tttatatctg
    19201 gcaatagcag tataaaaagc gcgccatagc ggctcaccgt gcaacaacac aacattaatg
    19261 cgttcaatga ggcccgattt tggcattagc cctggacggt ggaatccact tcacggaaat
    19321 gaaaacaaga acaagaaagg aagggttaaa acgaagaaat aaagaagagt atgaaatgga
    19381 tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt tttactggca
    19441 tttgcctgaa aagattcgat tcagcaccgc taaaacggca tttaccgctc gctgaacata
    19501 tcaggacaac atcgtgcccc aatgttcaac ccacggattt gattccgttt ccggttccgg
    19561 gttctcactg gcgtcaatca acagcatttc gccaacccgc tgagcgctct gttcctgcaa
    19621 caaggcatcg aactgtttgc cgccattgca gaaattcaca taactactgt cgccgagcgc
    19681 aatcacgcca taacgcagat tcggctggaa gcccagacta tctttgattc cctgaaagag
    19741 tggcacaata ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca ccagaacata
    19801 cttatcctga tagggcagcc agtcgcttaa ttcaggatct tcaaataccg ttgctttgtg
    19861 gccctgcgcg gtcagaatcg cttccgcttc ttcggccact aacagtgaat tcccgtacat
    19921 ggtgccgaca aaaataccaa tttccgccat accgttttct ccctagatta ggaacttatc
    19981 tctccatcct gacccgatgg cgcactgaac tcaacccttt cattttcagg aagcagaccg
    20041 caccagccaa attgtgataa tgcctgcatc caggtgtcgt ccaggcccgc gtgaatagcc
    20101 agcggctcgc cagtaaaagg atgcgtcaat gacaactgac tggcatgcag cattaaccgc
    20161 tgaaggccaa aatgctcagc accgctgcga ttctggcgta aatcaccatg tttgctgtcg
    20221 ccaataatcg ggtgccgcaa atgggcaaga tggcgacgaa gctggtgctt gcgtccagtt
    20281 ttcggctcca gttccaccag gccgtagcgc gtggtcgggt aacgtccagt cgctaccggc
    20341 atttctacgg tcgccagacc gcgataatgc gtcactgctg gctgcgggcc tttatcttcg
    20401 cgggcaaatt tatcagcgat tttgtccagt tcttccacca gtggataatc cagcaccgct
    20461 tcttccatca accagccacg cacaatcgca tggtaacgtt tctggatttg gtgctgttca
    20521 aactgttgtg ccagcagccg tccggcttcg ctggataatc ccatcaacaa cacaccagaa
    20581 gttggtcggt ccagacgatg agcagtaaaa acatgctggc ctatctggtc acgcacggtt
    20641 tgcatgacca ctactttctc gtcgcgatcc agccagctgc ggtgaaccag ccagccggag
    20701 ggtttattta ccgcaaccag ccattcatcc tgatagagta tttccagcat tagctcgcat
    20761 catccgcaaa aagagcatcc agtttttcca gctctgccag aataagcgcg cgttgcgggt
    20821 agtccgtcgc cagcgccatt tcataataag gcgcaacagc aaaagcgccc ggtaacggct
    20881 ggttgttatc taacaaatcg tgcattcgcg ggatcagcac ccactgcaac cactccagtg
    20941 gttccatggt gtccataaag aacggttgag tactgttaaa ttgatgcggc aggggttcat
    21001 cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag cgcctgcaac tggagacgaa
    21061 cgcggtcatg agtggtcata aagcaacctc aataagaaaa actgccgcga agaatagcag
    21121 gaataaaaaa agggagcact gtattcacag tgctcccggt tcgtttcgca gcattccggc
    21181 tacttttatt gctccctgct catccttgac aacttttcct ctggccttgc ggccaatcgt
    21241 tcatcctgaa ctattgcttc ctgctcacac caccccgatg tgatacttca tcctgaagtg
    21301 ttcctggcct tcctgaccca ccgaatcatc ctgaccggtt ctcattctcc atcctggagg
    21361 tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt tccctgaaac
    21421 accatcctgg tgtgtccttc agaagtgtca tcatcctgat gttcacttcg ttgtctgact
    21481 ccctgtcgac gaagatagaa tcgtcttttt ctcgaagtct tacaaggcgc ttacagacaa
    21541 tgccttaagg aaaatttcat atgaaagtta agaattaaca agcaacatgt tgtttttaaa
    21601 tgagaataac gattcttatc tttttaagta ttcgcttatt tcctggcgat ctcctacaga
    21661 gtctgtaaga gatctctcac aaccagatgg ggtatttgga ttacagaaga ggcttaagtt
    21721 gattaaggaa ttccgcaaga tttgaggcta aatgcgtccg tttacgtgtt cccagcgtct
    21781 ctttgcacac ttcaccggag agattacaca ccgagatgac ctctaactct tcttccagcg
    21841 tcgcgataaa tagcgtgggc ggcagtttta gccttttctg agtcaccaga tgaccaatca
    21901 gattttcctg cacccgacgg aagtcgtctt cactccaggt ttgcagcaat gtcagtttga
    21961 tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt cgtataaaag gtatgaatcg
    22021 tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt tacattttgt tccccggtaa
    22081 acggctgggg ttgccagtat acggcatctt cggtagttga aataatgcac ggtgaaggaa
    22141 cgccgtacag ttcctcactt aacggccagc ttttatgctc ttcatgccac gcatcgcagt
    22201 agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc gtccactaat ttctctcttc
    22261 acttaaacca gatacacttg tcgtttagtt tatctggttt atgacggtga aacatgtctt
    22321 cttatgcaaa ccatcaggca cttgcgggcc tgacacttgg aaaatcaacc gattaccggg
    22381 atacctatga cgccagccta ctgcaaggcg taccacgcag cctgaatcgc gacccgctgg
    22441 gtctgaaagc ggataacctg ccttttcacg gtacggatat ctggacgctg tatgaacttt
    22501 cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg tcatgttgaa cttgattaca
    22561 ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta tctcaacagt tttaaccaga
    22621 cgcgttttaa taactgggat gaggtgcgcc agacgctgga gcgcgactta agcacttgcg
    22681 cccaaggtaa ggttagcgtg gcgttatatc gtcttgatga actggaaggc cagccgatag
    22741 gtcatttcaa tggcacctgc attgatgacc aggatatcac tatcgataac tatgaattca
    22801 ctactgacta tctggagaat gccaccagcg gtgaaaaagt agtggaagag acgcttgtca
    22861 gccacctgct gaaatcaaat tgcctgatca cccatcaacc agattgggga tcgatccaaa
    22921 ttcagtatcg tggacgccaa attgacagag aaaaactgct gcgttacctg gtttctttcc
    22981 gtcatcacaa cgagttccac gaacagtgcg tggaacgcat ctttaatgac ctgttacgct
    23041 tctgccagcc agagaaattg agcgtttacg cacgttatac ccgtcgtggc ggtctggaca
    23101 ttaacccgtg gcgcagtaat aacgattttg tcccatcgac cacaagactg gttcggcaat
    23161 aaattttttc tcaattttgc gtgctggatt cacgcagaag gttgtgaaag gtcagcaggc
    23221 agggctattg taatcaaagg gaatgacgat attcgtccca taaggagttt ttcttgatta
    23281 cacatattag cccgcttggc tccatggata tgttgtcgca gctggaagtg gatatgctta
    23341 aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa ctgttcactt gccgtactga
    23401 actccggtag tttgaccgat aacagcaaag aattgctgtc tcgttttgaa aatttcgata
    23461 ttaacgtcct gcgccgtgaa cgcggcgtaa agctggaact gattaatccc ccggaagagg
    23521 cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg gttctgcgtg
    23581 acattctctt cgtttatggg caaattcata acaccgttcg ttttcccaac ctgaatctcg
    23641 acaactccgt ccacatcact aacctggtct tttccatctt gcgtaacgct cgcgcgctgc
    23701 atgtcggtga agcgccaaat atggtggtct gctggggcgg tcactcaatt aacgaaaacg
    23761 agtatttgta tgcccgtcgc gtcgggaacc agctgggcct gcgtgagctg aatatctgca
    23821 ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg tgctgcggtc ggacacgcgc
    23881 agcagcgtta caaagacagt cgctttattg gtatgacaga gccgtcgatt atcgccgctg
    23941 aaccgcctaa cccgctggtc aacgaattga tcatcatgcc agatatcgaa aaacgtctgg
    24001 aagcgtttgt ccgtatcgct cacggtatca ttatcttccc tggcggtgtg ggtacagcag
    24061 aagagttgct gtatttgctg ggaattttaa tgaacccggc caacaaagat caggttttac
    24121 cattgatcct caccggcccg aaagagagcg ccgactactt ccgcgtactg gacgagtttg
    24181 tcgtgcatac gctgggtgaa aacgcgcgcc gccattaccg catcatcatt gatgacgccg
    24241 ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt gaaagaaaat cgccgcgaca
    24301 ctggtgacgc ttacagcttt aactggtcaa tgcgcattgc gccagatttg caaatgccgt
    24361 ttgagccgtc tcacgagaat atggctaatc tgaagcttta cccggatcaa cccgttgaag
    24421 tgctggctgc cgacctgcgc cgtgcgttct ccggtattgt ggcgggtaat gtaaaagaag
    24481 tcggtattcg cgccattgaa aagtttggtc cttacaaaat caacggcgat aaagagatta
    24541 tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca gcatcgtatg aagttgccag
    24601 gctcagccta catcccttgc tacgaaatct gcacgtaatc tcccctcccc cggtgacgtt
    24661 tgttgccggg ttttctcatt tttggtcact tactcatcaa ctcatttcat ttgttatatg
    24721 aatgtttctt actaccctca cgcgacaaat atcaccacag ttaatatgtc atacaattta
    24781 tgttgaaacg caaacgtttc cctattttca taaaccatat tttattgcca tgaaaaagaa
    24841 attatcgcca tgattgacta aaagtattga ttttttcagt tcaacctaca tatattgcgc
    24901 gccccgtaag aagtcagatg tcgtttaatg ggtaaatatt gcccttaaat tctcttttac
    24961 ttttgattta cagagtaaag cgttgtgatt atctatcttc caagtagatt attttatttg
    25021 ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc aaattattac
    25081 ggtggaaaat gattaagcca tcgccgatag acagatttca tttttacggt caggcacctt
    25141 cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa atagatggaa
    25201 acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg caagacagac
    25261 accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct gttcctgcca
    25321 atcaacgctg gtgttggcgg tatgatcccg ctgatcatca tggctatcct tgcgttcccg
    25381 atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa aaacccgggc
    25441 gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa actgattact
    25501 ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc aatcaccaat
    25561 acagttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg tgcgattctg
    25621 tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct tcggtgagca gatgatcgtt
    25681 aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct ggctctgtac
    25741 ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac tgcatctgca
    25801 accggaaacg gtctgtggat gacactgtgg ctggcaattc cggtaatggt gttctcgttc
    25861 aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta cggcgatatg
    25921 gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt gctgaccgta
    25981 atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc tgcggctaaa
    26041 gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcgcc gatcatcgcg
    26101 tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg ccactacctg
    26161 ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa aggtaagtct
    26221 atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac gacctggatt
    26281 gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg cccaatcatc
    26341 gcgatgatcc tgttcctgat gccgatgtac gcaatccaga aagtaccggc aatgcgtaag
    26401 tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc aatctccgca
    26461 atcttctact cactgttcag ctaagtcctt tcgcgccgct ttcgggcggc gcttcctccg
    26521 ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat cggcattggc
    26581 ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac cgacgatctg
    26641 attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta cggctcgctc
    26701 tctctgaccg gtaaaggcca ccacacagat atcgccataa ttatgggtct ggcgggtaac
    26761 ctgccggata ccgtggatat cgattccatc cctggtttta ttcaggatgt gaatactcat
    26821 ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga tcagtgcatg
    26881 aacttccatg ccgacaacct ttctctgcat gaaaacggta tgcgcattac cgcgctggcg
    26941 ggcgataaag tcgtttacag ccagacttat tactctatcg gcggtggctt tattgttgat
    27001 gaagaacatt ttggtcagca ggatagcgca ccggttgaag ttccttatcc gtacagttca
    27061 gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg cctgatgatg
    27121 aaaaacgagc tggcgctgca cagcaaagaa gagctggaac agcacctggc taacgtctgg
    27181 gaagtcatgc gtggcggtat tgagcgcggt atttctaccg aaggcgtgtt acctggcaaa
    27241 ctgcgcgttc cacgccgtgc tgcagcacta cgccggatgc tggtcagcca ggataaaacc
    27301 accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc agtgaacgaa
    27361 gagaatgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg cgggattatt
    27421 ccggccgtgc tggcgtacta cgacaagttt atccgcgaag tgaacgctaa ctcactggct
    27481 cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc ctcgatttct
    27541 ggtgccgaag tcggctgcca gggtgaagtc ggcgtggcgt gctcaatggc ggcagctggt
    27601 ctggcagaac tgttaggcgc aagcccggcg caggtatgca tcgcggcgga aatcgccatg
    27661 gagcacaacc tcggtctgac gtgtgacccg gtcgccgggc aggtacaggt gccgtgcatc
    27721 gagcgtaacg ccattgcggc agtgaaagca gtaaacgctg cacgtatggc gctgcgccgt
    27781 accagcgagc cacgcgtctg cctcgataaa gttatcgaaa ctatgtacga aacaggtaaa
    27841 gatatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcaatgaa gatcgttgcc
    27901 tgcgattaat cgctctccag aggcctcttt ttgcgaggcc tcttcccgat ttctcatcca
    27961 gccatagcct gttctggcat cgaatgttac ccttatcgcc tgatctttaa gggggttatc
    28021 gtggctgttc atttgcttat tgtcgatgca ctgaatctta ttcgtcgcat tcatgccgtt
    28081 cagggttcac cctgtgtcga aacctgccag cacgcgctcg atcagctcat tatgcacagc
    28141 cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg ctggcgtcat
    28201 cagcgtttac cagattacaa agcgggtcga ccgccaatgc cggaagagtt gcacaacgag
    28261 atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc cgccagcggc
    28321 aacgaagccg atgacttagc cgccacgctg gcggtcaaag tgacacaggc cgggcatcag
    28381 gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac attacgtatt
    28441 cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga atttggcgtt
    28501 caaccgcagc agttgcccga ttactggggg cttgcgggga tcagcagttc aaaggtaccg
    28561 ggtgttgcag ggatcggacc aaaaagcgcc acacagctac tggtcgagtt tcagagtctg
    28621 gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa attagaaacc
    28681 cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga tttacatatc
    28741 gacggcaatt tacagcaatt gcggttggta cgataaagag atacagggtg ctaccgatta
    28801 acaatccatt aaatttaatg cacttgtagg ccggataaga cgcgccagcg ccgcatccgg
    28861 cattatcaca tccgcgcaat taccaggcgg tatggtaaag ctctacaata tcctcaagcg
    28921 ttgcttcacg cgggttgcca ccggtacaaa catcatccag tgccgcctgc gccagtgccg
    28981 gaatgtcttc cttgcgtaca ccaacatcac gtaaatgtgg cggaataccg acatcacggt
    29041 tgagtgcaaa caccgcttca acagcggcat tacgcgcctc ttccaggctc ataccttcca
    29101 ctttcacgcc cataacgcgc gcgatatcgc ggtacttctc accggtaaag tcagcgttat
    29161 agcgcatgac atgcggtaac aggatggcgt tcgcaacacc gtgtggcgtg ttgtaaaacg
    29221 cgcccagtgg atgcgccata ccatgcacca accctaatcc aacattcgag aatcccatac
    29281 ccgcaacata ctgcccgagc gccatttctt ctccggcatc cttatcacca gcaaccgatc
    29341 ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg cagcgcatcg gttagcgccc
    29401 acgcgccacg ggtaatatac ccctcaatag catgagtgag cgcatcgaca cccgtcgcgg
    29461 ctttcagcgc tggaggcata ccatccatca tgtcagcgtc aataaacgcc acctgcggga
    29521 tatcatgcgg atcaacgcaa acaaacttgc gccgtttctc ttcgtcagtg atcacgtagt
    29581 taatggtcac ttctgccgca gtgcctgctg tggtggggat tgccagaatc ggtacactgg
    29641 gtttattggt cggggaaagc ccttccaggc tacgcacatc ggcaaactcc gggttgttgc
    29701 tgataatgcc aatcgcttta cacgtatcct gtggagaacc accaccaata gcgatcaggt
    29761 aatccgcgcc gctattctgg aatacatcga gcccttcttt gacgacagta attgttgggt
    29821 taggtactac gccgtcgtaa atcgcccatg ccagtcctgc cacatccatc ttatcgatca
    29881 ctttcgccac cacgccgcat tgcaccagcg ttttatcggt gacgatcagc gccttctgat
    29941 aaccacggcg tttcacctca tcggttaaag acccaacagc cccccgacca aaccatgccg
    30001 tttcgttcag aatcattctg ttagccatca tccttctcct tgttgcttta cgaaattact
    30061 cttcaatccg taacccgtag gttttgaatt tctccagcac tacggcaatc tcttcatcgc
    30121 tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag gtaaagttgt gccagcactt
    30181 caacttcatg cgccagccat aacgctttct ccagattcgc ctcacaagcg ataagcccat
    30241 gatgttgtaa caaagttgcc ttacgatttt tgagagccag cgcaacatgt tcagaaagtt
    30301 cgcgtgttcc aaaggtcgca taaggcgcgc aaggaataga attaccgcca gccgccgcaa
    30361 tcatgtagtg aatcgcgggg atcggtcggt taagaatgga aactgccgta caatgaacgg
    30421 catgattgtg aacaaccgcg ttggcatccg gtctgctttg ataggccgcc atatggaaac
    30481 gccactcgct tgaggggagt tttccttcct catgtttgcc gttgccatca ataaagacaa
    30541 tatgcgactc cgtcagtttt tcatatggaa tacctgtagg cgtaatcagc atcccatcct
    30601 gataacgtac actgacgtta cccgctgtcc cctggttcag ccccaggcga gtcatttcca
    30661 ggcaagtgtc aataatctga cgagcaagtt tatttcgttc cattagctac ctctctctga
    30721 ttcaaaacag ggcaataatg ttgttccttt cacactattg aattagccgt ttaattaccc
    30781 accatcttct tcctgattaa ccaga
//
LOCUS       DAZFRG010000029        30170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFRG010000029 DAZFRG010000000
VERSION     DAZFRG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.029"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..144
                     /gene="tuf"
                     /locus_tag="ACHKUC_001035"
     CDS             <1..144
                     /gene="tuf"
                     /locus_tag="ACHKUC_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6617324.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="ACHKUC_001036"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="ACHKUC_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO6617325.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="ACHKUC_001037"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="ACHKUC_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO6617326.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="ACHKUC_001038"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="ACHKUC_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO6617327.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="ACHKUC_001039"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="ACHKUC_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6617328.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHKUC_001040"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHKUC_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO6617329.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="ACHKUC_001041"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="ACHKUC_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO6617330.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHKUC_001042"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHKUC_001042"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO6617331.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHKUC_001043"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHKUC_001043"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO6617332.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ACHKUC_001044"
     CDS             complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ACHKUC_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO6617333.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYTELKQLGLDGVMVYQETYNEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ACHKUC_001045"
     CDS             complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ACHKUC_001045"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO6617334.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ACHKUC_001046"
     CDS             complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ACHKUC_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO6617335.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ACHKUC_001047"
     CDS             complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ACHKUC_001047"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO6617336.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRANGCPVCGGCNADPV"
     gene            complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ACHKUC_001048"
     CDS             complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ACHKUC_001048"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO6617337.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAVDWRLATAQLLEIAGAGDE"
     gene            complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ACHKUC_001049"
     CDS             complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ACHKUC_001049"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO6617338.1"
                     /translation="MSATKLTRREQRALAQHFIDTLEGSAFPNSKRIYITATQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGIITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYVAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18154..18254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ACHKUC_001050"
     CDS             complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ACHKUC_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO6617339.1"
                     /translation="MLNQLDNLAERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18877..19650
                     /gene="nudC"
                     /locus_tag="ACHKUC_001051"
     CDS             18877..19650
                     /gene="nudC"
                     /locus_tag="ACHKUC_001051"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO6617340.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19690..20754
                     /gene="hemE"
                     /locus_tag="ACHKUC_001052"
     CDS             19690..20754
                     /gene="hemE"
                     /locus_tag="ACHKUC_001052"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO6617341.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVL
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20764..21435
                     /gene="nfi"
                     /locus_tag="ACHKUC_001053"
     CDS             20764..21435
                     /gene="nfi"
                     /locus_tag="ACHKUC_001053"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO6617342.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21478..22068
                     /gene="yjaG"
                     /locus_tag="ACHKUC_001054"
     CDS             21478..22068
                     /gene="yjaG"
                     /locus_tag="ACHKUC_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO6617343.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22255..22527
                     /gene="hupA"
                     /locus_tag="ACHKUC_001055"
     CDS             22255..22527
                     /gene="hupA"
                     /locus_tag="ACHKUC_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO6617344.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22540..23235
                     /gene="yjaH"
                     /locus_tag="ACHKUC_001056"
     CDS             22540..23235
                     /gene="yjaH"
                     /locus_tag="ACHKUC_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO6617345.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ACHKUC_001057"
     CDS             complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ACHKUC_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO6617346.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINALAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGDCGGGGHMGMGHW"
     gene            23894..25270
                     /gene="zraS"
                     /locus_tag="ACHKUC_001058"
     CDS             23894..25270
                     /gene="zraS"
                     /locus_tag="ACHKUC_001058"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO6617347.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMMGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25267..26592
                     /gene="zraR"
                     /locus_tag="ACHKUC_001059"
     CDS             25267..26592
                     /gene="zraR"
                     /locus_tag="ACHKUC_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO6617348.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ACHKUC_001060"
     CDS             complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ACHKUC_001060"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO6617349.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDISALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLAGNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ACHKUC_001061"
     CDS             complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ACHKUC_001061"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO6617350.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAVSNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLICGQWGERAPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            30096..>30170
                     /locus_tag="ACHKUC_001062"
     rRNA            30096..>30170
                     /locus_tag="ACHKUC_001062"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7078 a         7805 c         8308 g         6979 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     1981 gctgaaagaa ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aacgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgac cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccaggttcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtgga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2641 cacgtatgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag atgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg ggtctttttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaggtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     4741 gccgtaaact acctgcgaca atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagttg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgcattactg cgcgccatat tcgccagctg gaaaaagacg
     4981 acgtcaagct gatcgaagtt ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 acattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcata agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagctg
     5281 ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ctatgacttg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagttgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6061 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actccggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     6421 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgcggcg
     6481 acgtgctggc agacggtccg tccaccgatc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     6661 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 ctctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgatg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcgggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aaactcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     7801 tcgagcgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 atggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggcc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg tgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg cggtatgacc
     8701 aacctggaac gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggtgcg gaagcaatcc aggctctact gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga catcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gttgacgccc tgttggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gcgttgacta ctccggtcgt
     9301 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtga tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg
     9661 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtagcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggttt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta ctcatgaagg tatcatgatg actccggtta tcgaaggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat taacaaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgacaggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tatgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    12541 cttcacggaa tttttaagaa aggcagggcg aaacgaggaa gaagcttttc gccctggaca
    12601 acattcctgt tgacgggact accataaaaa aacacaacgc tttcaacaac cttgcgtttt
    12661 tcatcttccg gagctgctcg gaataaattt acgttcttac attctgtctc atggcctttg
    12721 cgatgggcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac cctgagcggt
    12781 taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact gttccagctc
    12841 ggggtggttg tcggcatagc cacctggctg cgttttcgaa aaagcgctga cgttattaat
    12901 tgccagcgga attacgcgat cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc
    12961 aatctccggt gcaagcagcc ggaaagcgca gatggcttgc actaactggc gttcatccat
    13021 aatcgacgca ggttcaatgc cgccagtaca tggacgcagg cgtgggaagg agacagagta
    13081 gcggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca tatagcagtc
    13141 aactcgccag ctgtcggaaa ggccgattag cgcgccgagg cctatcttat caatccccgc
    13201 acgccctagc cgatccggcg tttccagccg ccagaagaag tcctgttttt tacctttcag
    13261 atgatgatgg gcataagtcg cctcattata tgtctcctga taaaccatta ctccatcgag
    13321 gccaagctgt tttaactcgg tatattccgc ctccgccagc ggttgcactt ccatctgtag
    13381 tgaagagaac tgttcacgca gggcagggag atgacgacga aagtaatcca tccccacttt
    13441 cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc gtatagcggc
    13501 gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcggt tgctcataga
    13561 aaatccgcaa taagtacagt cgttagcgca aagattggaa agataaagcg ggacgtagaa
    13621 gctgacgacg ttgccaaatc gctgacgagt cagacgttgc gctcgctggg ccagtggttc
    13681 cagatagcca ctggcggcgg gtgatagcaa tgccatcatg tcgtcgcggg tgagttgcga
    13741 ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca ggtggatgtc
    13801 atcccagtcc agttgtcgcc agcgatcgct gaaggttttc atgccgatgc ctccagaaat
    13861 ccagtcagcg ggctggtggc atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt
    13921 gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac gggatcgtcc
    13981 gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag cgcctgcgcg
    14041 gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaacggtggc ctgctggata
    14101 ataatctcca gcatggcgcg ggtttccagt ccctgattcg agccaatcgg cgcgccgagc
    14161 ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg atcggctccg
    14221 cagtaaggca gcacgataaa tccctgttgt accagcgttt cggcggcttt cagggtttcg
    14281 atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag ccagtttgtg
    14341 cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgcggt tttcgccccg
    14401 gaagtattcg gcagcagggt cacacccgcc gcgataagcg gttcgaggat agcgtcgttg
    14461 tgctggcgca agtcgacacg tttcatcgcc agcgtcacca gttggctgcc ggaagcgcga
    14521 atagactcca ccatcagttg tgaagaggcg aattttccgg tgccagtaaa cagatgtgaa
    14581 tcaaacgttt tgtccgcaat atgtaacatc tcaaccccct gcaataactt gaaaaagcag
    14641 gatctggtcg ccatcctgca cgatatgttg cgcccattgt tcccgcggga cgatttgctg
    14701 attaatcgct agtgccgcac ctgcttgttg ttgatcgagt tgctccagca gttcgtgaac
    14761 agttagtccg gcgacacact gcatcggttg atcgttaaac aggatctgca ttgcatcctc
    14821 cacataccgg gcaaccgttg gcgcggcgca acgtcaggct gcgccactgg ctcgatttac
    14881 cgtcgaacag tcggagttct cccgcaggcg tctctatacc gcttaataac ttaatcgctt
    14941 ccagtgcctg taaagtgccc ataaccccga ccaccgggcc aaccacgccc gccgtgcggc
    15001 agttgcgttc tggctcctgg ttatctggcc acaggcagcg gtaacatccc tgctcccagg
    15061 gcggcgtcag taccatcaac tgcccgccaa atccgaccgc gctggcggtg ataagcggcg
    15121 tgttgagtgc cacgcaggtg gcattaatct cctggcgagt cgccatattg tcagtacagt
    15181 cgagcaccac atcggcttgt gcaaccgcat cttttaacgc ctcacccgtt aaccgttgtt
    15241 gtagtgccgt cagttgaatg tcgggattca actgtgtcag tcgctgttgg ctgacctgtg
    15301 atttcgggcg atcgatatct tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat
    15361 gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccccgcc aggtatagcg
    15421 cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg agcagttttt
    15481 gctgcccgtc cagagcgata tcgtcgagca ggatttggcg gctataacgc ataaagtcac
    15541 ggtcattcat cgccagctcc tgcaatttcc agcaactgtg ccgttgccaa acgccagtct
    15601 acggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc tatcaccgca
    15661 ggcgcgcgcg ccagactgat accgccaatc gccacggtgg gataatccgc caggcgctca
    15721 acatgccgtg ccagctgttc cagtccctgc ggtgcagaag gcatctgttt ggtttgcgtt
    15781 gggaacacat gtcccagcgc gatataagaa ggacgtgccg ccagcgcgac gtcgatctcc
    15841 atatcgtcat gagtcgaaac gcccagccgc aggcccgctg cacggatggc attgagatcg
    15901 gtggcttgca aatcttcctg ccccagatgg acgccatacg cctgatgctt aatcgccagc
    15961 cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc
    16021 acgacatcgg cttccacctc ttcatcgcgc cgatctttga tgcgtagctg gagagtacgt
    16081 acgcctgcat ccaacagacg ttcgatccac tgtacgctgt caaccaccgg gtacagtcct
    16141 aaacgaaagg gtacaggagg aaactcaggc tgatacatca cgcttcctcc ttacgcaggt
    16201 agatttctcc gcctctggcg cggaagttct ccgacatatc cgccattccc acttcaatgg
    16261 tttgcgcggc gacgtaatca cgcacttcct ggctgatttt catcgagcag aatttcggtc
    16321 cacacatgga gcaaaaatgg gcgactttac cggactcttg cggcagggtt tcatcgtgat
    16381 aagcgcgggc agtaaacggg tcgagggcca gattaaactg gtcttcccag cgaaattcga
    16441 agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct ttcgccaggt
    16501 cggcggcgtg agcggcaatc ttataggtga taatcccttg tttaacatct tctttatttg
    16561 gcaggcccag atgctctttc ggcgttacgt agcagagcat cgcgcagcca aaccagccaa
    16621 tcatcgccgc gccaatccct gacgtgaagt ggtcatagcc cggcgcgata tcggtagtta
    16681 gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc
    16741 ggcgaatcat ctgcatcggc acgtggcctg ggccttcaat catcacctgc acgtcatatt
    16801 cccaggcgat tttagtcagt tcacccagcg tatgcagctc ggcaaactgt gcttcatcgt
    16861 tggcgtcctg aatagaaccg gggcgcaggc cgtcgcccag cgatagcgaa acgtcataag
    16921 cagcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc tgatgatggg
    16981 agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc aggcgtttcg
    17041 cggtcatcgg cacgtagcgc agcagcaccc ccgcatggat agtgaagtaa tccacacctt
    17101 gctcggcctg ttccagcagg gtgtcgcgga acgcttccca ggtaagatct tcggcgatcc
    17161 cgttcacctt ctccagtgcc tggtagatcg gcactgtacc gatcggcacc gggctgttac
    17221 gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccagt ggaaagatcc atcacggtat
    17281 ccgctcccca gcgcgtggac cataccagtt tttccacttc ttcttcgatg gaagaggtga
    17341 ccgctgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca ataatcatcg
    17401 gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca gcaacttcat
    17461 cacggacaaa ttccgcagtg atattttccg gaagacgtgc gccaaagctc attcccggat
    17521 gctggtggcg taaaacttcg ctacgtatgc gctcgcgacc catattctcg cgaatggcga
    17581 tgaattccat ttcgggcgtg atgataccct ggcgggcgta gtgcagttgg gtgacgcggc
    17641 gtcctgcttt ggcgcgtttt ggcgttaata cgccgctaaa acgcagctcg tcgaggccat
    17701 catctgccag ccgcgctttt gtgtaatcgg aactgcgcac ggtaagttct tcggtatcgc
    17761 cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg ttaatggcga
    17821 tctgcggatc gccatacgga ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt
    17881 actgcggttg ttctttgcta ccgccgatta gcgtcgggct aagctggatc tcacgcatcg
    17941 gcacgcgcac gccgggttgt gtggcagtga tgtaaatgcg ttttgagttg ggaaaggcgg
    18001 agccttccag ggtatcgata aaatgttggg ccagggcgcg ttgttcgcgg cgggtcagtt
    18061 ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga cgacggatga
    18121 agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt tcccttcgca
    18181 ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca gttaaggcac
    18241 tccgacaaga aattagcccc acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc
    18301 agaactcaag caggatattt gacgcgggcg gcgtcaagca ccagtagaat cagcttatct
    18361 tccagcacaa agcgcgcttc cagtgcctcg ccgatgtcag ataaaacctg ttgaaactca
    18421 aggtaattat catgatcgat ggcggtttcc agactggaat cgtagtcatc cataatctgt
    18481 tgagtattgg cttcgagttg tggccaaatc ttcgcggcgc gtgcgagttg cccattgcct
    18541 tccagcttat gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc
    18601 aggctctgac aaaaatcatc aagggctttt tcgtttagcc tcatgtacga ttctttgcca
    18661 ggcttaatgc caaccagatt gtagtaagcc acgagcagat gcttacgtac atgtagccag
    18721 cgatcaacca gtttgttact tcctctgacg cgctccgcca ggttatcgag ctggttaagc
    18781 atgattgact ccgcaagttt gtattcaaaa actgctctgt gagaaatgta aaaaccatgt
    18841 taaacatgcc agtgatgcaa aggtagtgca agagctatgg atcgtataat tgaaaaatta
    18901 gatcacggct ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa gggagaattg
    18961 ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa
    19021 tggcaggggg aacctgtttg gttagtacaa ctgcagcggc gtcacgatat ggggtcggta
    19081 cgtcaggtca ttgatctcga tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg
    19141 gcggagtttt accgatcgca taaatactgt ggttactgcg ggcatgaaat gtatccaagc
    19201 aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc
    19261 ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc
    19321 catcgtaacg gtgtccatac agtacttgcc ggattcgtcg aagtgggtga aaccctcgag
    19381 caggcagtcg cgcgggaagt gatggaagag agcggaatta aagttaaaaa cttgcgttac
    19441 gtgacctccc aaccgtggcc gtttcctcag tctttaatga ccgcgtttat ggcggaatat
    19501 gacagcggcg agatcgtgat cgacccgaaa gaattgcttg aggcgaactg gtatcgctat
    19561 gacgatttgc cgttactccc gccgcccggc accgtagcgc gccgtctgat agaagataca
    19621 gtggcgatgt gtcgggcaga gtatgagtga tgatacactg accgcctgac gcactaagga
    19681 acagccaaaa tgaccgaact taaaaacgat cgttatctgc gggcgctgct gcgccagccc
    19741 gttgatgtca ctccagtatg gatgatgcgc caggcgggtc gctatctacc ggaatataaa
    19801 gccacgcgcg cccaggcggg cgattttatg tcgctgtgca aaaatgccga gctggcgtgc
    19861 gaagtgactt tgcaaccgct gcgtcgctat ccgctggatg cggcgatcct cttttccgat
    19921 atcctcaccg tgccagacgc gatgggttta gggctctatt ttgaagccgg agaaggtccg
    19981 cgttttacct cgccagtcac ctgcaaagcc gatgtcgata aactgccaat tccggacccg
    20041 gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc
    20101 gaagtgccgc tgattggttt ttccggcagc ccgtggacgc tggcgaccta catggtggaa
    20161 ggcggcagca gcaaagcgtt caccgtgatc aaaaaaatga tgtatgccga tccacaggcg
    20221 ctgcacgcgc tactcgataa actggcgaaa agcgtcactt tgtatctgaa tgcgcagatt
    20281 aaagccggtg ctcaggcagt catgattttc gacacctggg gcggcgtgct taccgggcgc
    20341 gattatcaac agttctcgct ctattacatg cataaaattg ttgatggttt actgcgtgaa
    20401 aacgacggtc gccgcgtacc ggtcacgctg tttaccaaag gtggcggaca gtggctggaa
    20461 gcgatggcag aaaccggttg cgatgcgctg ggcctcgact ggacaacgga catcgccgat
    20521 gcgcgccgtc gcgtgggcaa taaagtcgcg ctgcagggta atatggaccc gtcgatgctg
    20581 tacgcgccac ctgcccgcat tgaagaagaa gtcgcgacta tacttgcagg tttcggtcac
    20641 ggtgaaggtc atgtctttaa ccttggtcac ggcattcatc aggatgtgct gccagaacat
    20701 gctggcgtgt ttgtggaggc agtgcatcgc ttgtctgaac agtatcaccg ctaaggagcg
    20761 attatggatc tcgcgtcatt acgcgctcaa caaattgaac tggcttcttc tgtgatccgc
    20821 gaggatcgac tcgataaaga tccgccggat ctgatcgccg gagccgatgt cggatttgag
    20881 cagggcggcg aagtgacgcg agcggcgatg gtgctgctga aatacccctc gcttgagctg
    20941 gtggagtata aagtggcccg catcgccacc accatgcctt acattccagg ttttctctcg
    21001 ttccgcgaat atcctgcgct gctggcagcg tgggagatgc tgtcgcaaaa gccggattta
    21061 gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc ttggcgtggc cagccatttt
    21121 ggcttattgg tggatgtgcc gaccattggc gtagcgaaaa aacggctctg cggtaaattc
    21181 gaaccgctct ccagcgaacc gggcgcgctg gccccgctga tggataaagg cgagcagctg
    21241 gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac cggccatcgg
    21301 gtcagcgtgg acagcgcgct ggcgtgggta caacgctgca tgaaaggcta tcgtctgccg
    21361 gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca
    21421 gcaaatcagc cctaattcag gtaaactgcg gataatttcc gtatttgaga actcaacatg
    21481 ttacaaaacc caattcatct gcgtctggag cgcctggaaa gctggcagca cgtcactttt
    21541 atggcctgct tatgcgaacg tatgtacccc aattacgccg tattctgtca gcaaaccggc
    21601 ttcggcgatg ggcagattta ccgccgcatt ctcgatctca tttgggaaac gctgaccgtc
    21661 aaagacgcaa aagtaaattt cgacagccaa ctggagaaat ttgaagaagc gattccttca
    21721 gccgacgatt tcgatctgta cggcgtttat ccggcaatcg atgcttgcgt ggcgttaagt
    21781 gaactggtcc attcgcgttt aagtggcgaa acgctcgaac acgcggtgga agtgagtaag
    21841 acttccatca cgaccgtggc gatgctggaa atgactcagg ctggtcgcga aatgagcgat
    21901 gaagagctca aagaaaaccc agctgtagag caagaatggg acattcagtg ggaaatattc
    21961 cgacttttag ctgagtgcga agaacgcgat atcgagctga taaaaggcct tagggcagac
    22021 ctgcgtgagg cgggtgagag caatattggt ataatttttc agcaataaga ccagaaaacg
    22081 tgatttaacg cctgatttgt cgtacctgga gtcttccctt tcgccccccg tctggtctac
    22141 atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg ctattctggc
    22201 atttccgtcg cactcgatgc ttagcaagcg ataaacacat tgtaaggata acttatgaac
    22261 aagactcaac tgattgatgt aattgcagag aaagcagaac tgtccaaaac ccaggctaaa
    22321 gctgctctgg agtccactct ggctgcaatt actgagtctc tgaaagaagg cgatgctgta
    22381 caactggttg gtttcggtac cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac
    22441 ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg taccggcatt tgtttctggc
    22501 aaggcactga aagacgcagt taagtaagat tgcgtggcag tgaacagttt taacgaaggg
    22561 gtggtttcac cccttttgtc tttctggcgt cgatcattga tgctggcagg cgctctgttt
    22621 ctcactgcct gtagtcacaa ctcttcactt cctcccttta ccgccagtgg atttgctgaa
    22681 gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca tctgcttgcc
    22741 gtgtttagcc cgtggcgcag tggcgatacc accacgcgag agtatcgctg gcagggcgat
    22801 aaccttacgc tcatcaatat caatgtttac agcaaaccgc cggtgaacat tcgcgcgcgt
    22861 tttgacgatc gcggcgatct gagctttatg caacgtgaat ccgatgggga aaagcagcag
    22921 ctttctaacg accaaatcga tttataccgt tatcgtgctg atcagatccg ccagattagc
    22981 gatgccttac gtcaggggag agtcgtgctg cgccaggggc gctggcatgc gatggaacag
    23041 accgtgacca cctgcgaagg gcaaaccatt aaacctgatt tagattcgca ggcgatagcg
    23101 catatcgagc gccgccagag ccgctcttct gttgatgtca gcgtggcatg gctggaagcg
    23161 cccgaaggtt cgcaattact gttggtggca aactctgatt tctgtcgctg gcaacccaac
    23221 gagaaaacgt tctgatttac cagtggccca tacccatatg accgccacca ccgcagtcgc
    23281 cgtaacccat tccggtaccg cgcggaatac ccgcttcagc catcgcaata tctcgtttca
    23341 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgagc gcgttaattt
    23401 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc tttgtcacca
    23461 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    23521 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccatgt ccgccgtgag
    23581 caaatgccga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    23641 tcgtgttccg tttcatggtt catcctccag tggttgtctt acttcgggta ttgcatcttc
    23701 cgtgccaacg gcgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    23761 actcgcctgt tgtgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    23821 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    23881 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    23941 cgcgatcctc cccgtggtca ttgttgggct ggtggggctg tttgcggtga cagtgattcg
    24001 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24061 tatccgtgct ctcgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24121 gcagcaggca ttactggaag aaatggccgg gcagcctggt gtacgttggt ttgcggtcac
    24181 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atgatgggaa aacagcttta
    24241 ttccccgcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    24301 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    24361 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caatttttat
    24421 cgctttcgac gccagtaaca ttgtgagtgc tgaagatcgt gagcagagaa acaccctgat
    24481 tatcctcttc gccctggcga cggtcttgct ggcaagcgtg ttgtcattct tctggtatcg
    24541 ccgatatctg cgctcgcggc agctattgca ggatgaaatg aagcgcaaag agaagctggt
    24601 ggcactgggg catctggcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    24661 taaagggctg gcgaaatact ttgccgaacg cgcgtcagca gggggagaag cgcatcaatt
    24721 ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg agttgctgga
    24781 actggttaag ccaacgcatc tggctttgca ggcggtggag ctcaacacgc tgattaacca
    24841 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    24901 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    24961 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25021 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcgga
    25081 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggactggg
    25141 gctggcggtt gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25201 ggagggaaaa ggcgcaacgt tcaccctttg gcttccggtc aatattacgc gtaaggaccc
    25261 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    25321 actattttgc aggctttgct gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    25381 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    25441 atggcagaga tggatggtat cgccacgcta aaagagatca aaacattaaa ccccgcgatt
    25501 ccggtgctga ttatgaccgc gtactccagc gtcgagacgg cggtagaggc gttgaaaacc
    25561 ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    25621 aaggcgctcg cgcatacaca cagtgttgac gctgaaacgc ctgcggtgtc tgccagccag
    25681 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    25741 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    25801 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgc
    25861 gcggcgctca acgaatcctt gctggagtct gaattgttcg gtcacgaaaa aggggcgttt
    25921 accggggccg acaaacggcg ggaggggcgc tttgttgagg cggacggcgg cacgttgttt
    25981 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctacg tgcgattcag
    26041 gagcgcgaag ttcagcgtgt cggtagcaac cagactattt cggttgatgt ccggctgatt
    26101 gcggcgactc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26161 tactatcgcc tgaacgtggt ggcgattgaa gttccgtcgc tgcgtcagcg gcgggaagat
    26221 attcctctgc tggcaaacca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    26281 aaaggtttta cgccccaggc gatggatctg ctgattcact acgactggcc gggaaatatt
    26341 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    26401 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggtaca aagtcaggat
    26461 attcagccgc tggtggaagt cgaaaaagag gtgattcttg cggcactgga gaaaacgggc
    26521 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct cttggcaaaa
    26581 ctgtcgcgtt agttctgctc gcgttcgata gcgcgccagc cgatatcttt ccggcagaag
    26641 cagtcgtccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgctacg
    26701 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggtcactac ctgctcgtta
    26761 cccgccagtt tcgtgcccgc gtggaacact ttgccgtcag acacttcttc cagcggcagg
    26821 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    26881 ccgagagaag cgcgttcatc ccactcggac gttttctcgt ccagcttgcc ttcacaggcc
    26941 gccaggcaga gttcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27001 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27061 cccgcgtaga gaaaaccagt gtaggtgttg ccttctgccg ccatgccttt cacggttggc
    27121 cagatgattc gttccatcgt gcgctgatga acctcatcgg tcactactgg cgcaggggag
    27181 taagcgccca tcccgccggt gttcggcccg gtatctttat cgcctacgcg tttgtgatcc
    27241 tggctggtgg ccatcggcag cacatgctcg ccgtctacca tcacgataaa gctcgcttct
    27301 tcgccgtcga ggaactcttc gataacaatg cgatgacccg cgtcgccaaa cgcgttgccc
    27361 gccagcatat cgcgcacggc ggcttcagct tcttccagcg tcatcgccac gataacgcct
    27421 ttcccggcgg ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    27481 gccagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat tttatggcgg
    27541 gccaggaaat ctttggtaaa cgctttcgag ccttccagtt gcgcggcacc tgcggttggg
    27601 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    27661 tccgggccga cgatggttag atctaccttt tcgttttgtg cgaaatccag cagcgccgag
    27721 atatcggtta cgccgatagc gacgttctgt agggcgggtt ccagcgcagt gcctgcgtta
    27781 cccggagcaa caaaaacagt ttcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    27841 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    27901 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    27961 cgtcatcacg gatagaaccg ccaggctgga ttacacaggt cacacccgca gcggcggcgg
    28021 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28081 ccagaccttc atcggcggct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28141 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagcg ttggatttca
    28201 cgaacttcgc cactttccag cagaacagcg catcacgcaa ttcctgttcg gtcggctggc
    28261 gtttggtgac gacgcgcagt tcttctgcac cgaccatccc caggtcgcga tcctgaacca
    28321 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggagc acgctcgccc cactgaccgc
    28381 agatcaggac gcgaacgttc tgtttagcgg cggttatttt cagggcttct tcgctggcgg
    28441 acggcgcaat aatcacttca acaaactgac gagaaatgat ggcctgcgcg gtttccgcat
    28501 ccagctcgcg gttaaaggcg ataatgccgc cgaatgcgga ggttgggtcg gttttatacg
    28561 cgcgatcgta agcatcgaga atggagttgc tgaccgccac gccgcatggg tttgcatgct
    28621 tcacgatcac acaggccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    28681 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggtg gcaacggagg
    28741 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtagc
    28801 gcatatcctg cttcttgatg aagttcaggt tcagcgtacg cgggaagcga ccggcggctt
    28861 ctttgctttc accgtgatag gccggaacca tgctgccgaa gtagttggca atcatgctgt
    28921 cgtaggcggc agtgtgttcg aaggctttga tggcaaggtc gaaacgggtt gcaagcgtca
    28981 gcgatccttc gttggcatcc atctctttaa taatggcgtc gtagtcgcta ctcttcacca
    29041 cgattgcgac atctttatgg ttcttggcgg cggagcgcac catcgttggg ccgccgatat
    29101 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcgacggtc tgggcgaacg
    29161 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29221 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    29281 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    29341 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    29401 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    29461 ctggacgacg ttgttgcatg gtaaatcccc tggatttgac tattacagag agcgttagct
    29521 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    29581 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    29641 ttttgacagt ggctaccgtg ggaaaaatac agaaattaca ttgaagattg tggataactc
    29701 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcattttta
    29761 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgcctcca tcgacacggc
    29821 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    29881 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg agctgaaagc
    29941 cgcgtcgcaa ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    30001 gattcttgac gtcgcaagac gaaaaatgaa taccaagtct caggagtgaa cacgtaattc
    30061 attacgaagt ttaattcttt gagcatcaaa cttttaaatt gaagagtttg atcatggctc
    30121 agattgaacg ctggcggcag gcctaacaca tgcaagtcga acggtaacag
//
LOCUS       DAZFRG010000030        29838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFRG010000030 DAZFRG010000000
VERSION     DAZFRG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.030"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..1290
                     /gene="yebS"
                     /locus_tag="ACHKUC_001063"
     CDS             7..1290
                     /gene="yebS"
                     /locus_tag="ACHKUC_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO6617351.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            1259..3892
                     /gene="yebT"
                     /locus_tag="ACHKUC_001064"
     CDS             1259..3892
                     /gene="yebT"
                     /locus_tag="ACHKUC_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO6617352.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            3972..5411
                     /gene="rsmF"
                     /locus_tag="ACHKUC_001065"
     CDS             3972..5411
                     /gene="rsmF"
                     /locus_tag="ACHKUC_001065"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO6617353.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            5530..5766
                     /gene="yebV"
                     /locus_tag="ACHKUC_001066"
     CDS             5530..5766
                     /gene="yebV"
                     /locus_tag="ACHKUC_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO6617354.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            5871..6062
                     /gene="yebW"
                     /locus_tag="ACHKUC_001067"
     CDS             5871..6062
                     /gene="yebW"
                     /locus_tag="ACHKUC_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO6617355.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDIYNTEQQCLYSMSDQLIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(6063..6719)
                     /gene="pphA"
                     /locus_tag="ACHKUC_001068"
     CDS             complement(6063..6719)
                     /gene="pphA"
                     /locus_tag="ACHKUC_001068"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6617356.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNHQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     ILWSRSRLGEHQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(7114..7455)
                     /gene="yebY"
                     /locus_tag="ACHKUC_001069"
     CDS             complement(7114..7455)
                     /gene="yebY"
                     /locus_tag="ACHKUC_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO6617357.1"
                     /translation="MMRKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(7468..8340)
                     /gene="copD"
                     /locus_tag="ACHKUC_001070"
     CDS             complement(7468..8340)
                     /gene="copD"
                     /locus_tag="ACHKUC_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO6617358.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(8344..8718)
                     /gene="yobA"
                     /locus_tag="ACHKUC_001071"
     CDS             complement(8344..8718)
                     /gene="yobA"
                     /locus_tag="ACHKUC_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO6617359.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8857..9087
                     /gene="holE"
                     /locus_tag="ACHKUC_001072"
     CDS             8857..9087
                     /gene="holE"
                     /locus_tag="ACHKUC_001072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO6617360.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9189..9845
                     /gene="yobB"
                     /locus_tag="ACHKUC_001073"
     CDS             9189..9845
                     /gene="yobB"
                     /locus_tag="ACHKUC_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO6617361.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFLS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9869..10531
                     /gene="exoX"
                     /locus_tag="ACHKUC_001074"
     CDS             9869..10531
                     /gene="exoX"
                     /locus_tag="ACHKUC_001074"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO6617362.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10528..12588)
                     /gene="ptrB"
                     /locus_tag="ACHKUC_001075"
     CDS             complement(10528..12588)
                     /gene="ptrB"
                     /locus_tag="ACHKUC_001075"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO6617363.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVRRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12797..13456)
                     /gene="yebE"
                     /locus_tag="ACHKUC_001076"
     CDS             complement(12797..13456)
                     /gene="yebE"
                     /locus_tag="ACHKUC_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6617364.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHINAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(13783..14139)
                     /gene="yebF"
                     /locus_tag="ACHKUC_001077"
     CDS             complement(13783..14139)
                     /gene="yebF"
                     /locus_tag="ACHKUC_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO6617365.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(14206..14496)
                     /gene="yebG"
                     /locus_tag="ACHKUC_001078"
     CDS             complement(14206..14496)
                     /gene="yebG"
                     /locus_tag="ACHKUC_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO6617366.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            14630..15808
                     /gene="purT"
                     /locus_tag="ACHKUC_001079"
     CDS             14630..15808
                     /gene="purT"
                     /locus_tag="ACHKUC_001079"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO6617367.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(15864..16505)
                     /gene="kdgA"
                     /locus_tag="ACHKUC_001080"
                     /gene_synonym="eda"
     CDS             complement(15864..16505)
                     /gene="kdgA"
                     /locus_tag="ACHKUC_001080"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6617368.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(16542..18353)
                     /gene="edd"
                     /locus_tag="ACHKUC_001081"
     CDS             complement(16542..18353)
                     /gene="edd"
                     /locus_tag="ACHKUC_001081"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO6617369.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLTRIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(18588..20063)
                     /gene="zwf"
                     /locus_tag="ACHKUC_001082"
     CDS             complement(18588..20063)
                     /gene="zwf"
                     /locus_tag="ACHKUC_001082"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO6617370.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            20401..21270
                     /gene="hexR"
                     /locus_tag="ACHKUC_001083"
                     /gene_synonym="yebK"
     CDS             20401..21270
                     /gene="hexR"
                     /locus_tag="ACHKUC_001083"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO6617371.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            21398..22840
                     /gene="pyk"
                     /locus_tag="ACHKUC_001084"
     CDS             21398..22840
                     /gene="pyk"
                     /locus_tag="ACHKUC_001084"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO6617372.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(22972..23943)
                     /gene="lpxM"
                     /locus_tag="ACHKUC_001085"
                     /gene_synonym="msbB"
     CDS             complement(22972..23943)
                     /gene="lpxM"
                     /locus_tag="ACHKUC_001085"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6617373.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(24062..25384)
                     /gene="mepM"
                     /locus_tag="ACHKUC_001086"
     CDS             complement(24062..25384)
                     /gene="mepM"
                     /locus_tag="ACHKUC_001086"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO6617374.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(25400..26332)
                     /gene="znuA"
                     /locus_tag="ACHKUC_001087"
     CDS             complement(25400..26332)
                     /gene="znuA"
                     /locus_tag="ACHKUC_001087"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO6617375.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            26411..27166
                     /gene="znuC"
                     /locus_tag="ACHKUC_001088"
     CDS             26411..27166
                     /gene="znuC"
                     /locus_tag="ACHKUC_001088"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO6617376.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            27163..27948
                     /gene="znuB"
                     /locus_tag="ACHKUC_001089"
     CDS             27163..27948
                     /gene="znuB"
                     /locus_tag="ACHKUC_001089"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO6617377.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(28093..29103)
                     /gene="ruvB"
                     /locus_tag="ACHKUC_001090"
     CDS             complement(28093..29103)
                     /gene="ruvB"
                     /locus_tag="ACHKUC_001090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO6617378.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(29112..29723)
                     /gene="ruvA"
                     /locus_tag="ACHKUC_001091"
     CDS             complement(29112..29723)
                     /gene="ruvA"
                     /locus_tag="ACHKUC_001091"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO6617379.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         7171 a         7644 c         7495 g         7528 t
ORIGIN      
        1 cacgtcatgg ctcttaacac accacaaatt acgccgacaa agaagataac agtgagggct
       61 atcggcgagg aactgccgcg tggtgattac caacgttgcc cgcaatgtga catgctgttt
      121 agcctgcccg agataaattc tcatcaaagt gcctattgtc cgcgctgtca ggcaaaaatt
      181 cgtgacgggc gcgactggtc gctaacgcgc ctggcggcaa tggccttcac catgctgttg
      241 cttatgccgt ttgcctgggg cgaaccgctg ttgcatatct ggctgttagg cattcgtatt
      301 gacgccaacg ttatgcaagg catctggcaa atgaccaaac agggcgatgc gataacgggg
      361 gcgatggtct ttttctgcgt tatcggtgcc cccctcattc tggtgacctc catagcttat
      421 ttatggtttg gtaaccgatt gggaatgaat ttacgcccgg tactgctgat gcttgagcgg
      481 ctgaaagagt gggtgatgct ggatatctac ctggtcggca ttggcgttgc ttctataaag
      541 gtacaggatt atgcccatat ccaggcgggt gtcggcttgt tctcttttgt ggcgttggtg
      601 attttaacga cggtgacgtt gtcacatctt aatgttgaag aactgtggga gcgattttat
      661 ccgcagcgcc ccgctacgcg tagggacgag aaactccgtg tctgtcttgg gtgccatttt
      721 accggctatc ctgatcagcg tggtcgctgc ccgcgttgcc atatcccgct acgcctgcgt
      781 cgccgtcata gtctgcaaaa atgctgggcg gcgctgttag cgtcaatcgt tttgttgtta
      841 cctgccaacc tgttgcctat ctctatcatt tatctgaatg gtggtcggca ggaagatacg
      901 attctttccg gaattatgtc gctggcaagt agcaacattg cagttgcggg aatcgtgttt
      961 atcgccagta ttctggtacc gtttactaaa gtgatcgtca tgttcacttt actgttgagt
     1021 attcatttta aatgccagca aggtttacgc acacgcattc tgttactgcg gatggtgacc
     1081 tggattggtc gctggtcgat gctcgacctg tttgtcatat ctttaaccat gtcgctgatt
     1141 aatcgcgatc agatcctcgc ttttactatg ggaccggctg cgttttattt cggcgcagcg
     1201 gtaattttga ctattcttgc tgtggaatgg ctggacagcc gcttactttg ggatgcacat
     1261 gagtcaggaa acgcccgctt cgacgactga agcgcagatt aaaaataaac gccgtatctc
     1321 acctttctgg ctgctgccgt tcatcgcgct aatgattgct ggttggctga tttgggacag
     1381 ttatcaggac cggggcaata ctgtcaccat cgactttatg tcggcggatg gtattgttcc
     1441 aggccgtacg cctgttcgtt atcagggcgt tgaagttgga acagtgcagg atatcagcct
     1501 cagcgacgat cttcgtaaga ttgaagtcaa ggtcagcatc aagtccgata tgaaagatgc
     1561 gctgcgcgaa gagacacagt tctggttggt gacgccaaag gcctcgttgg caggtgtctc
     1621 cgggctggac gccctcgtcg gtggtaatta tatcggcatg atgccgggta aaggtaaaga
     1681 gcaggatcac tttgtcgcac tcgacaccca accgaaatat cggctggaca atggcgatct
     1741 gatgatccac ctgcaagccc ccgatctcgg ttcactgagc agtggttcat tggtctattt
     1801 ccgcaagatc ccggtgggta aagtctacga ctatgccatc aatcccaaca agcaaggcgt
     1861 ggtgattgat gtcctgatcg agcggcgttt taccgatctg gtgaaaaaag gtagccgttt
     1921 ctggaacgtt tccggcgttg atgccaacgt cagtatcagt ggcgcgaagg tgaaactgga
     1981 aagtctggcg gcactggtta acggtgcgat tgccttcgat tcacctgaag agtcgaaacc
     2041 tgccgaagcg gaagatacct ttggtctgta tgaagacctg gcccatagcc aacgtggcgt
     2101 aataataaaa ctggaactgc caggcggtgc cggattaacc gccgactcga cgccgttaat
     2161 gtatcagggg ctggaagtcg gacagctgac taaactggat ttaaatcctg gtggtaaagt
     2221 cactggggaa atgaccgttg atcccagcgt cgtcaccctg cttcgtgaaa atacccgcat
     2281 cgaattacgc aacccgaaat tatcccttag cgatgccaat ctcagcgccc tgctgaccgg
     2341 aaaaaccttt gagttggtgc ccggcgatgg cgagccacgc aaagagtttg ttgttgtgcc
     2401 aggcgaaaaa gccctgctgc aagagccgga tgttctgaca ctgaccctga cagcaccaga
     2461 aagttacggt attgatgctg gtcagccgct cattcttcac ggcgtgcagg taggccaggt
     2521 tatcgatcgt aaactcacca gcaaaggcgt cacctttacc gtcgccatcg agcctcagca
     2581 tcgcgaactg gtaaaaggcg atagcaaatt tgtcgtcaac agccgtgtcg acgtgaaggt
     2641 ggggctggat ggcgttgagt ttctcggtgc cagcgcctca gaatggatca atggcggaat
     2701 acgtattctg ccgggcgata aaggcgagat gaaagccagc tatccactgt atgccaatct
     2761 ggaaaaagcg ctggagaaca gccttagcga tttacccacc acaaccgtga gtttgagtgc
     2821 agagacgctg ccggatgtgc aggcaggatc ggtagtgctg taccgtaaat ttgaagttgg
     2881 tgaagttatt actgtgcgtc cgcgagctaa cgcgtttgat atcgatctgc atattaagcc
     2941 ggagtatcgc aaccttctga ccagcaacag cgtgttctgg gcagaaggtg gggcgaaagt
     3001 tcagctgaat ggtagtggtc tgactgtaca ggcatccccg ctctccagag cattaaaggg
     3061 ggctattagc ttcgataacc tcagcggtgc cagcgccagt cagcgtaaag gcgacaagcg
     3121 tattctgtat gcttccgaaa cagcggcccg tgcggttggc gggcaaatta cgcttcacgc
     3181 tttcgatgcc ggaaaactgg cggtcgggat gccaattcgc tatctcggta ttgatatcgg
     3241 gcaaatccag acgctggatc tgattaccgc gcgcaatgaa gtacaggcaa aggcggtgct
     3301 ctatccggaa tatgtccaga cctttgctcg cggtggtacg cgcttctcag tggtcacacc
     3361 gcaaatttcg gcagctggcg ttgagcatct tgatactatc ctccagccgt atatcaacgt
     3421 cgaaccaggc cggggcaatc ctcgccgcga ctttgaatta caagaggcca ccattactga
     3481 ttcgcgttac ctggatggct taagcattat tgttgaagcg ccggaagccg gttcgttagg
     3541 tatcggtaca ccggtgctgt tccgtggtct ggaagtaggt acggttaccg gaatgacgct
     3601 ggggacattg tcagatcgcg tgatgattgc gatgcgcatc agtaaacgct atcaacacct
     3661 ggtgcgtaac aattccgtct tctggttggc atcgggttac agtctggact ttggtctgac
     3721 gggcggcgta gtgaaaaccg gtacctttaa ccagtttatc cgtggcggca tcgccttcgc
     3781 cacgcctccg ggtacgccac tggcaccgaa agcccaggaa ggcaagcact tcctgttgca
     3841 ggaaagtgaa ccgaaagagt ggcgtgaatg gggaactgcg cttcccaaat aatgcccact
     3901 gctccggcgt gcctgcgccg gagcgtttat gctaaactgc gcgcctgttt ttttgccagt
     3961 ggtatatgct cgtggcccaa cacaccgttt atttcccgga cgcctttctg acacaaatgc
     4021 gcgaagcgat gccttcgacg ctctcttttg atgattttct tgccgcctgt cagcgcccgt
     4081 tgcgccgcag cattcgcgtt aatacgctga aaacctccgt tgctgatttc ctgcaattaa
     4141 ctgctcctta tggctggacg cttacgccaa ttccgtggtg tgaagaaggt ttctggattg
     4201 aacgcgacaa tgaagatgca ttgccgttgg gtagtaccgc cgagcattta agcggcctgt
     4261 tttatattca ggaagccagt tcaatgttgc ctgttgccgc cttgtttgct gacggtaatg
     4321 caccacagcg ggtgatggat gtcgctgctg caccaggttc caaaacgacg caaattgctg
     4381 cgcggatgaa taacgaaggg gcaatccttg ccaatgagtt ttccgccagt cgggtaaaag
     4441 tgttacatgc caatatcagc cgctgtggca tcagtaatgt tgcgctcacg cattttgatg
     4501 gccgcgtatt tggtgcggca gtgccagaaa tgttcgatgc cattttgctg gacgctccct
     4561 gctccggcga aggcgtggtg cgtaaagatc ccgatgcgct aaaaaactgg tcaccagaaa
     4621 gcaatcagga aatcgcagcg acccaacgag agctgatcga cagcgccttt catgcattac
     4681 gtcctggcgg tacgctggtt tactcgacct gtaccttaaa ccgggaagaa aacgaagccg
     4741 tttgcctgtg gctgaaagag acttatcccg acgcagtaga gtttttaccg cttggcgatc
     4801 tcttccctgg tgcaaacaag gcgctgaccg aggaaggctt tttgcatgtt ttcccacaaa
     4861 tttacgactg cgaaggcttc ttcgttgctc gtctgcgtaa aactcaggcg attcccgcct
     4921 tacccacccc aaaatacaaa gtgggcaatt tcccgttcag cccggtgaaa gatcgcgaag
     4981 ccggacaaat tcgtcaggcg gctgcaagtg ttggcttaaa ctgggatgaa aacctgcgac
     5041 tctggcagcg tgacaaagaa ctgtggttgt tcccggtggg cattgaagcc ctgatcggta
     5101 aagtccgatt ttctcgcttg gggattaaac ttgccgaaac gcacaacaaa ggttatcgct
     5161 ggcagcatga agccgttatt gcccttgcct cccccgacaa tgtgaacgct tttgaactga
     5221 caccgcagga agcggaggag tggtatcgcg ggcgcgatgt atacccgcat gccgcgccag
     5281 tggcggatga tgtattggtt accttccagc atcagccgat tggtttagcc aaacggattg
     5341 gttcacgact gaaaaacagc tacccgcgtg aactggtgcg cgacgggaaa ctttttaccg
     5401 gtaacgcctg acagcgcaca aaaaaaagcg cactttttga ctggcacatt caactgcctc
     5461 aactaggctg aaaaatggtg cgatcggact ggtcgtacca caaccggcag ctaaatggag
     5521 agcacgaaga tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta
     5581 cacggcgaat cgccgggtga tcgaacgtta accattcctt gtaaatcaga ccccgattta
     5641 tgtatgcaac tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa
     5701 cactctgtcc tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt
     5761 gcctgatcca aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt
     5821 gtaaaatctc tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc
     5881 tggtactttt tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatatctaca
     5941 acacggaaca gcagtgtctc tattctatga gcgatcaact gatccgtcat ggcggttgtt
     6001 ttccgattga ggattttata gatggtttct ggcgacctgc acaggagtac ggtgattttt
     6061 aattattgca attgcacaag agtcagttct cccccaaaga cagcaccggt atcaatataa
     6121 tgcaggttgc caatatccac gcgatggcgc aaaggtgtat gaccaaacca gaaatgatca
     6181 gcacctgcaa ttccctgccc tttttggtgt tcacctaatc gcgagcggct ccacaagatc
     6241 tgatgcaaat cgacgtcctt ttgccattca taaacatcat ctggataatc ggcatgagca
     6301 ataacatgtt tgccggtgcg actgtgtact tcaagaataa agggcaaatg ctgacatttt
     6361 tccagcgccg ttttcgcttg tttctggtga ttatctgcca gcgcaataaa ccagtcgccg
     6421 ccattcatca accataaaga catctgccgg gatgccagcg catccatcgc catctgttca
     6481 tgattgcctc ttaccgcacg aacccaatgt tgttccagta actgcaggca acgtaaactt
     6541 tgcggcccac gatcgataac gtctcccact gagataagta aatctcgcca cggatcaaaa
     6601 cgacaatgcc ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc gcctgaaagc
     6661 cagatatgtc gccattgatg acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc
     6721 aatattttcc tcccgcgcta aagatcacat aatcttaaca agaatgttaa aaaacgctgg
     6781 actcagacag tagagtgtgt gttatggttg actataaagt cagcgaaggg aatgcttctg
     6841 gcttttaaca gataaaaaga gaccgaacac gattcctgta ttcggtccag ggaaatggct
     6901 cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg ccttgctctt
     6961 taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg
     7021 tggtggtcat cagcttaaat gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt
     7081 ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag ctcgctctat
     7141 aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct gaatcaccga
     7201 aatgggttgg gctttagtct tcccactggc aacttccttt tgtgcgatat cgtttaaagg
     7261 atactgcacg agggtacttg ggttaatgac atacaaagca ttacccggtc ggcaagtcag
     7321 catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac ggctgacggt
     7381 aatcacctgc ggtgccgcca gcgcagctgc agaactggtg agtaacagaa acgccagaat
     7441 actttttctc atcattttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat
     7501 gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaga caaaccaggg
     7561 agccgcacga tcttcatcct gcctcatgcg cggtacaaga acataacgat tagccagcgc
     7621 gatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt
     7681 cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttgccagtac
     7741 gccgatcacc gcaaaatgcc cgcaccagga aaaacgcatc agcgcctgaa tagcctggtg
     7801 tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca
     7861 cgccgccgca cagatcaggt gaattgcgtg attagtctga tggattttcg ccgttacccc
     7921 ttcattcaac gtcgcatgcc cgactcccgc cagcagaata aattgcgcgg tagtgagcat
     7981 aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc
     8041 gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag ccaaccaaat
     8101 gtttggcgaa aatacatctg accatcccgc tcccatcaac ccgccctgaa cggcgagcat
     8161 tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt
     8221 cgtcagcaag cgacgaatcg ttaagggggc cagccaggca ccgtacattg cgaagccaaa
     8281 aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat
     8341 gatttatttc acactaaagg tgtaatgtcc tttcgtttta tgcccatcca ccgaaacaac
     8401 atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg
     8461 cttttggtcc tgctcattcc gcttcgccgg taatgtttta atattttcgt tttttggccc
     8521 cgtgattttt gcaccactga atcctgtttc aacgccttcc gagaagttta aagtgattgc
     8581 ctgcggagct gctgtcactt gtgcatttgc cgcaggatac tgatgcgtta aatgcgcatg
     8641 tgcccagacg gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga
     8701 gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta cagagcataa
     8761 ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcaag gctcactctt
     8821 agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggctaaactg
     8881 gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggcaggggt ggcatttaaa
     8941 gaacgctaca atatgccagt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg
     9001 cgcagctggt ttcgcgagcg gcttatcgcc caccgtttgg cttcggtcaa tctgtcacgt
     9061 ttaccttacg agcccaaact taaataaaac ttatacatag ttacactttc ttacataacg
     9121 cctgctaaat tatgagtatt ttctaaaccg cccccataat ttgcagtcat tttgaaaagg
     9181 aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac
     9241 tcaccgaaca ggtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc
     9301 agctgttagt ttttctatcg ctttctttac tggggtgtga ttattcgcga cgtgcccttc
     9361 ctgccccacc cgatctctcg ctattagacc cgctttgtta tgccgcaacg acctggcgaa
     9421 tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg
     9481 tattcgcacc gtggcgaaaa acgccaggga tctaccatca gagttatggt gcatgtctgg
     9541 gccgtcgttc cagaaccatc acggtggtcg atgaacaacc gcaaggtatg gatatggacc
     9601 cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg ctggcgtcgg
     9661 cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg gcaaatgccc
     9721 gtggtaacag tgcactatgg gatgaacatg gtcgtctcat cgttcgcgcc gatcgtggtt
     9781 cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac
     9841 gctaggcttt ttcggcctgg agcatgccat gttgcgcatt atcgatacag aaacctgcgg
     9901 tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa
     9961 tcccatgagc cacctggtgc gtcccgatcg tcctattagt ccacaggcga tggcgattca
    10021 tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga tcccgcacta
    10081 ttacggtagt gaatggtatg tcgcgcataa cgccagcttt gaccgccgcg tactgcctga
    10141 aatgcccggt gagtggattt gcactatgaa actggcccgt cgtttgtggc cagggatcaa
    10201 gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc cgccgggcct
    10261 gcatcatcac cgcgcgttgt atgactgtta tatcaccgct gcgttgctta tcgatattat
    10321 gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc catcgttgat
    10381 gacgactttc acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga
    10441 tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg agctgcgttt
    10501 aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta atgttccctg
    10561 cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt taaagcgacc
    10621 agatttaccg ccatgacctg agtccatgtc ggtacagagc aataaaagat ggtcatcggt
    10681 tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc
    10741 gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatacgg
    10801 gctgtagctt ttcatatact cgtaatattg cggatcctgc ggattccccc actcttcaaa
    10861 ttcgccggtg gtaagaggaa ttgattcatc cagcattgtt gttacaacat caacaaacgg
    10921 tacctgggcg ataacgccgt ggaacaattc cgggcgttga ttaatcgcaa cgcccatcaa
    10981 catacccccc gcactcccgc ccatcgcata acagagcgaa ggagagccat agcccagttt
    11041 taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt
    11101 accgtcctcg taccattgtt gccccagctc accgccgcca cgaacatgga caatggcgta
    11161 gacaaatcca cgatccaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc
    11221 gccgtaagaa ccgtagccat acaccagcag cgggttgtgt cctttgcgaa aatgtttgcg
    11281 atgatagacc agcgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc
    11341 actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata cacgacgctc
    11401 accggtatcc atatccagtt caaacaaagt atctggtgtg gtcatggaag aataaccata
    11461 acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caagtcacat aggccggatc
    11521 atcaaaggca atgccaatga cttcccgggt cttgcggtta atttgccgca aactggttaa
    11581 cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat
    11641 aatgttatca cgtggcggaa ttaactcttc ccactgttgc tcatcacgca tacgggtacg
    11701 gtataagcca aagtttttac cgtggcggtt ggaacgcaga taaaaccgat gctggtagtg
    11761 atcaaggctg tattcgtgat ctttgcggcg aggcagaaaa acaaacggct cggcatcggc
    11821 catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccagat gaatgaccac
    11881 ataatgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta
    11941 aatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccggacct gataagggag
    12001 cagcgtcact ggatgcttgc gaacatagta gaaagtccag gagtcatttg cccagacaaa
    12061 gctgggttca acgttatcca gcagttccgg gtaccagtta cctgtttcca gattacgaaa
    12121 acgaatgccg tactgccgtc gggaaagaaa atcttctgcc agtgccataa tggtgttatc
    12181 tggcgtaatc gccattccgc ccatcgaata aaactcacta tgagccgcgc gcttgttggc
    12241 atcgagcaat gtttcccact cgtcccactc ttcactgaac gccgattgac gctggtagat
    12301 agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg
    12361 cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa
    12421 ggcttgttgc gaggccatca cccgatgacc gtaactattt tcttgttgca gatagtccag
    12481 gacttccggc tgagaacgcg tatcgtcccg cagccagtag taattatcga tgcgcgtatc
    12541 gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt
    12601 tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc gaaacagggt
    12661 agtcattgct tagatgatga caggtaatgg cgcggatatc gactgttatg cagacagaga
    12721 aaatcagcct gttcaaatgg ctgtgcggtt ctggataacc cgaaatagtc aacttcaggc
    12781 tatccagaga gcggaattac tccgccaaag tacgtttttg ctgttcgaga tcgcgttcaa
    12841 tgccttcacg aacgtcctgg gggattttca gcgcatcacc cagtgcattc aggtaactgc
    12901 gttccataaa atggtcaata tcaatagccg cgcagctcag gaaatagatt tccagcgcct
    12961 cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct
    13021 gctcaatgag tacacgcccc ttctcttcca caccggcttc tcgcaattgc tggtcgattg
    13081 ccgcacgttc tttggcattg atatggccat cacttttagc ggcaaagacc agcgcaagga
    13141 tcaaacgttc cgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcatcct
    13201 gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc
    13261 cgccgccaac cagtaacgcg tttgtgccat atttcgtaag aagtttacgt gctgatttat
    13321 ttgcgaccag cagcccagcc agtccgccta atgcgccagg cactaaaagt ttgcccaatc
    13381 cctgatccgc agacgaggag gtagaagaac ggctttgccc aagcagagat tgcagttgat
    13441 ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta gcatagcgga
    13501 tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc
    13561 cgttattcaa agaaattcgc gccaggtaag gtgttcactg tatataaggt gataagggta
    13621 ttttaggtga aagtagtttg acattttgag tggaactgtt tgatgctgat tgcccagttc
    13681 gacgctactg cgtcttctca ggactacgat tgtgttgcgt tgtagggcgg acaaggcact
    13741 cgcgccgtat ccgccaacgg tcaatcgaat tatcagaagc cattaacgcc gctgatattc
    13801 cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac ttttaccacg
    13861 cacggttagc ggtactgacc atttatcatc tttcccctga atgtcctgca aactgaccca
    13921 tgccacgggg tcggcctgac cgacaatttt ttgatcatca gcccagcgcg ccacgcgatt
    13981 ttgttggtaa tcacgtttta cgctcgcggc aattccggca gcatccagac cttcacactt
    14041 tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg cgcaggcaga
    14101 aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca tagcacaatg
    14161 attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga
    14221 ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt tgccggtttt
    14281 cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg cttcacgttg
    14341 ctcatcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat cggccgtatc
    14401 aagcatcttg tcataagcat cggcttcctt tttgctggta aacgacattt tctcttcgcc
    14461 ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat aataactgtg
    14521 attttataca gtatatttct tttcagttgg gaaatcaaca tcagcaataa agacacacgc
    14581 aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta tgacgttatt
    14641 aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg gtgaactggg
    14701 taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta
    14761 tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata tgcttgatgg
    14821 tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc cggagatcga
    14881 agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg ttgtcccctg
    14941 cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga
    15001 gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaaaccttt tccgcgaagc
    15061 ggttgccgcc attggctacc cctgtattgt taaaccggtg atgagttctt ccggtaaagg
    15121 gcagacgttt attcgttctg cagagcaact tgctcatgca tgggagtacg ctcagcaagg
    15181 tggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat
    15241 taccctgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg taggtcatcg
    15301 ccaggaagat ggcgactatc gtgaatcctg gcaaccacag caaatgagcc cgcttgccct
    15361 tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt atgggctgtt
    15421 tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaagtct cccctcgccc
    15481 gcatgacacc gggatggtga cgctaatttc tcaggatctc tcggagtttg ccctgcatgt
    15541 acgtgccttc ctcggacttc cggttggcgg gatccgccag tatggtcctg ctgcttctgc
    15601 cgttatcctg ccgcaactga ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt
    15661 aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca gccgtcgtct
    15721 gggggtagca ctggctactg cagagagtgt tgttgacgcc attgaacgcg cgaagcacgc
    15781 cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa aaatgcccga
    15841 tcctcgatcg ggcattttga cttttacagc ttagcgcctt ctacagcttc acgcgccagc
    15901 ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag ccaggaacca
    15961 ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg agaaatacca
    16021 cctgtcgggc agaaacggac ctgagagaac ggacccgcga tcgcctgcag ggctttcaca
    16081 ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc catacccagc
    16141 atcagttcgg aaacagtgct gatccccgga atcagaggaa tagtcccttc ggtagcagct
    16201 ttcagcagcg gctcggtcag acccgggcta attgcaaact gtgcacctgc ttcagtgact
    16261 tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc aggcacttct
    16321 ttggcgatag cacggatagc gtcaactgcg cactcggtac gcagagtcac ttccagaacg
    16381 cgcaccccac cagcaaccaa cgcttttgcc atcggcaccg cgtgttccag tttttttacc
    16441 acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt tttccagttt
    16501 ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt gatacaggtt
    16561 gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc acgtcctgtt
    16621 cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg
    16681 tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc
    16741 acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc tgacggcact
    16801 ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa acaccggtcc
    16861 aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt cgcttttggc
    16921 ccctgatgac ggacaacaac gacacaatcg cggtccagta aacccgcttc aaaggccggc
    16981 ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg gttctctacc
    17041 ggcacggcag aggttttcat aaccgctcgg cccaggttac cgcttaacac ttttgtccca
    17101 ccatgatgag agaaaggttg ttcgaaggac gcgatcacat tgttgtcgag tgatttttct
    17161 gccccttccc gccagtccag ttcaccatta ttcagccatg gttcaagggt ataacgagac
    17221 agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt gagcagttca
    17281 cgtaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc cggaccgttc
    17341 gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc atcccagtta
    17401 atctgaatac cggctgcgcg cgccatcgcc actaggtgca tggtgtggtt agtggaacca
    17461 ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat catcttaccg
    17521 atcggcatcc attcattacc attaccggtc atgcgcgtaa cctggcgcgc agctgcagcg
    17581 gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg caactgcatc
    17641 cccataaact ccaccaccat ctggttggtg ttggcagtac cgtagaaagt acatgttccc
    17701 ggcgcatgat aagacgcggc ttctgactcc agtaatgcca tgcggtccac tttaccttcg
    17761 gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc catcggtcca
    17821 gacggcacaa acaccgcagg caaatgacca aacgacaggg ctgccatggt cagacctggg
    17881 acaatcttgt cgcacacacc gagaaacaga gcaccatcaa acatgttatg ggacagcccc
    17941 accgccgcag acatcgcaat cacttcgcgg cttaacagcg acaattccat tccatcctgc
    18001 ccctgggtga caccatcaca catcgccgga acaccacccg caacctgacc aaccgcattc
    18061 gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg gtgagcggag
    18121 agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct tttcaaagag
    18181 gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc
    18241 gaacgatgaa cggtcgaagt tttcgcttgt tctatccggg tgaggtaagc agagcgagtc
    18301 tcgcgcgaac gttcaatgat tcgatttgtt acgcgtaaca attgtgggtt cataaaggct
    18361 cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcatacat ggaaattatg
    18421 attgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa
    18481 aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct acaatctgtg
    18541 caagatcatg ttaccggtaa aataaccata aaggatgagc gcagatatta ctcaaactca
    18601 ttccaggaac gaccatcacg ggtaatcatc gccactgagg caacgggtcc ccaggttccg
    18661 gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat ggagtctacc
    18721 catttccagg cttcttccac ttcgtcgcga cgtacaaaca gtgcctgaat accacgcatg
    18781 gtttccagca gcaaacgttc ataggcatcc gccagatgcg tctgattaaa ggtttctgaa
    18841 tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta
    18901 ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag tttattttgc
    18961 ggtagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa atagaccacg
    19021 acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg cacaccggcc
    19081 cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt gctgctcttg
    19141 ttcgcgccct cttcttccag atagcccggc acttttttgc cctgagcgaa gcccgcagta
    19201 tattgcccgc gtacggtttt ttcgcgcaca ttggagcggt cgatgcggcg cagagacttc
    19261 agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagacgg cggagacatc
    19321 gcaatcatgc aaagaatttg cagcaggtgg ttctggatca tgtcgcgcat ctgaccggct
    19381 ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt aatctcaaca
    19441 tgatcaatgg tgcgattgtc ccagttgttc acaaacagag agttagcaaa acgcagcgcc
    19501 aacaggttca gcactgtttc tttaccaaga tagtggtcga tacggtaaac ctggcactcc
    19561 tcgaagtatt cgccaacctg atcgttgatt tcctgcgagg tcgccagcga cgtccccagc
    19621 ggtttttcca tgactacgcg tgccggttta gcattcagtt ttgcctcacc aagccctttg
    19681 caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat acgatttttt
    19741 tgatccagca tcgcgccgag acggctgaat gcagcagtgt cattgacatc aagattacaa
    19801 aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc tttcatgaaa
    19861 gtttcgagcg cctcgcggac aactttggta taagccgctt tatcccagtc agcacgccct
    19921 acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg atacagggaa
    19981 ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc
    20041 tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg gtacttaagc
    20101 cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc ttgcgaaaac
    20161 tgtaaacgct tatccacccg tgcgattacg gtaaaagcgc gcaaagtgcg gcaaaactga
    20221 taaaaaaatc atcgttttcc attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa
    20281 ataacaactt ttttatctgc ttttgtcatt aacacagcac acaggcgtaa tatctgacaa
    20341 aactgcattt cgatttcttt cagagcggaa atcgtcatta cccgtgagtc tctttacatc
    20401 atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc tgagcgcaaa
    20461 gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctctg
    20521 gcgctggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacaca
    20581 cgcggttttc ctgattttaa acttcatctg gcacaaagtc tggcgaatgg cactccctat
    20641 gttaatcgca acgtcaatga agatgacagc gttgaatcat acacggggaa aatatttgag
    20701 tccgcaatgg caacgctcga tcatgtccgt cattcactgg ataaatctgc catcaaccgc
    20761 gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ttcttcagcc
    20821 gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc
    20881 gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg
    20941 ctgatttctc acacgggaag aacaaaaaat ctggtcgagc tggcgcagct ggcacgcgaa
    21001 aacgacgcta tggtaattgc cctcacctct gcgggtaccc cactcgcccg ggaagcaacg
    21061 ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga
    21121 cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg gcgcggtaca
    21181 aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag
    21241 cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg
    21301 acatccgaat gagattaatt tatcgccatc acggcgttat ttcattcgga tttcatgttc
    21361 aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac
    21421 taaaatcgtt accacgttag gcccggcaac agatcgcgat aataaccttg aaaaagttat
    21481 cgcggcgggt gccaacgttg tacgtatgaa tttttctcac ggctcgcctg aagatcacaa
    21541 aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct
    21601 gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct
    21661 caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga
    21721 aaaagtcggt atcgactaca aaggcctgcc tgctgacgtt gtgcctggtg acatcctgct
    21781 gctggacgat ggtcgcgtcc agttaaaagt actggaagtc cagggcatga aagtgttcac
    21841 cgaagtgacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg
    21901 tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat
    21961 tggcgtagat tacctggctg tctccttccc acgctgcggc gaagatctga actatgcccg
    22021 tcgtctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga
    22081 agccgtttgc agccaggagg caatggatga catcatcctc gcctctgacg tggtaatggt
    22141 tgctcgcggc gacctcggtg tggaaattgg cgatccggaa ctggtcggca ttcagaaagc
    22201 gttgatccgt cgtgcacgtc agctaaaccg cgcggtaatc acggcgaccc agatgatgga
    22261 atcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt
    22321 tctggatggt actgacgctg tgatgctgtc ggcagaaact gctgctggtc aatacccgtc
    22381 agaaactgtt gcagccatgg cgcgcgtttg cctgggggcg gaaaaaatcc cgagcatcaa
    22441 cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcca ttgccatgtc
    22501 agcaatgtac gcggctaacc acctgaaagg cgttacggcg atcatcacca tgaccgaatc
    22561 gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctgccaattt tcgccatgtc
    22621 acgacatgaa cgtacgctga acctgactgc tctctatcgc ggcgttacgc cggtgcactt
    22681 tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc tgcgtgataa
    22741 aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt
    22801 gggttctact aataccacgc gtattttaac ggtagagtaa gtacgttgcc ggatgcggcg
    22861 aaaacgcctc atccggccta cagttcaatg ataattcaac agatttcgaa tcttctgaag
    22921 caaacttgaa cttatcatca gtcgaaggcc tctcctcgcg agaggcattt tttatttgat
    22981 gggataaaga tctttgcgct tatacggttg gatttcgccc ggtttgcgag ttttcagcaa
    23041 ttttaatatc caggtgtatt gttctggtcg cggaccaaca aaaatctcga cttcttcatt
    23101 catccgccgc gcaatcgtat gatcatccgc ctctaacaga tcatccatcg gtgggcgcac
    23161 ctgaatcgtc agacgatgcg tcttgccatc ataaatcgga aacagcggta caacgcgcgc
    23221 acggcacact ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa
    23281 atcaacaaat tcgctgtgtt ctgggccatg atcctgatcg ggtaaataat atccccagta
    23341 accctgacgt accgactgga tgaatggttt aataccatca tttctcgcat gcagacgacc
    23401 accaaagcga cggcgcaccg tgttccagac ataatcaaaa accgggttgc cctgattatg
    23461 gaacatcgct gccattttct gcccttgcga ggccatcagc atggcaggaa tatcgacggc
    23521 ccaaccgtgc ggcaccagaa aaatcacttt ctcgttatta cgccgcatct cttcgatgat
    23581 ctccagccct tgccagtcaa cgcgcggctg aattttctcc ggcccgcgta ttgccaactc
    23641 agccatcatt accatcgctt gcggcgcggt ggcaaacatc tcatctacaa tcgcttcgcg
    23701 ttcagcttca ctacgttctg gaaagcagag cgacagattg attaacgcac gacggcgtga
    23761 gctttttccc agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat cacggaactt
    23821 tggcggcgtt aaagcgatac ccgccatcgc tgctacgccc agccatgctc cccagtagcg
    23881 cgggtggcga aaggatttat caaactcagg aatgtattcg ctattatttt ttttcgtttc
    23941 catgcttttc cagtttcgga taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac
    24001 ttgccccgca ccaaataaaa aagccggtac tgactgcgta ccggctgcga atggatgtta
    24061 attaatcaaa ccgtagctgc ggcacaatct ctttggcctg tgccaggaat tcgcgacgat
    24121 cggagccggt cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct
    24181 ggtttatcca tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa
    24241 gcgcgatacg gtcgccacgt ttcaccttct gtcccggttt caccaggatc ttgcgcaagt
    24301 gcatataacg cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc
    24361 cactacgttt ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccttgtg
    24421 gcatggcaaa atcaacacct ctgtgtggcg caacgcgacc ggtcaccgga ttagtacgac
    24481 gcgggttaaa gttagatgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt
    24541 tcgccagacc agtaccgtta cgatcataga atttgccatc ttcagcgcgg attgcataat
    24601 aatctttacc ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat
    24661 caagcatttc tcgtgacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat
    24721 ccatttgcca ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt
    24781 ttctggcgct ggcaacaaag cttcccccga cggtaccttt cagcagattg ttgacccact
    24841 ctccttgctg catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc
    24901 gggtttcacg acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg
    24961 tccaggagag ttgttgaccg attttcaggt tacgcaattc tttgtcggca gcagccagtt
    25021 gggtgatatc acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag
    25081 tggaaacaac atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct
    25141 ggggaatggc ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga
    25201 gcgaacgaat ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcacggt
    25261 gataaacata gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca
    25321 taacgcggtg tggtcgcggt aaattattaa acgctagggc gacagagcgg gctatctgtt
    25381 gcacgtaatc acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt
    25441 gattcaggaa ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca
    25501 acgttcccat acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact
    25561 gtggctcagc aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta
    25621 aacgctgcgc gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc
    25681 cgaactgttt ttcgaagtag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga
    25741 gcggcgcgag ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct
    25801 tcaggttggc gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga
    25861 ttgcaaccgc tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc
    25921 cgtgatggtg atcttcgtca cttttttccg cgtgatcgtg atcatcatca tcgtcgtgaa
    25981 tacttttcat cagcagcggt ttcacatctt ctagctgcgc aatcgttacc tgtttcgctt
    26041 ctggtaattt acttaccggt ttttgcatga acgcttccat ctccgggcca acccaaacga
    26101 ctaagtccgc gttctgtaag cgttttacat ccgatggacg cagtgaataa tcatgttctg
    26161 aagcgccgtc aggcagtaaa acctgcgttt ctgttacccc atcagcaatg gcagaagcga
    26221 tgaacccaac gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcaccgc
    26281 cccagagagc ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac
    26341 caatcatcgt aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac
    26401 taaaattaac atgacaagtc tggtttccct ggaaaatgtc tcggtttctt ttggccaacg
    26461 ccgcgtcctc tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg
    26521 gccaaacggc gcaggtaagt cgacactggt acgggtagtg ctcggactgg taacacccga
    26581 tgaaggggtt atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta
    26641 tctcgacacc acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggcacaca
    26701 taaagaagat attttgcctg cactgaaacg tgtccaggcc gggcatctga ttaacgcacc
    26761 gatgcaaaag ctctcgggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa
    26821 tcgaccgcaa ttattagtgc tggatgaacc cactcagggc gtggatgtga atggtcaggt
    26881 ggcgttatat gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt
    26941 atctcacgat ctgcatctgg taatggcaaa aaccgatgaa gtgctttgcc tgaatcacca
    27001 catttgttgt tccggcacac cggaagttgt ttccctgcat ccggagttta tttctatgtt
    27061 tggtcctcgt ggtgctgaac aactgggtat ctatcgccat catcataatc atcgtcacga
    27121 tttacaggga cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt
    27181 cccggttggt tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta
    27241 gtctggcgtc gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggcgtc
    27301 gcgtttggtt tgttgctgga cgtgaatcca ttctatgcgg tgattgcagt tacgctgctg
    27361 ctggcgggcg gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta
    27421 gggattatgg cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat
    27481 attcgtgttg atttgatggc ttacctgttc ggtgatttac tggcagtgac gccagaagat
    27541 ctcatctcta ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc
    27601 aatttgctgt cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag
    27661 cgcgtgaaat tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattc
    27721 gtcggcgcgt tgattattac ttcactgctg attattcctg ctgctactgc acgtcgcttt
    27781 gcccgcacgc cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtgact
    27841 ggcggtttaa ccttttccgc attttacgat acacctgcag gcccgtcggt ggtgctatgc
    27901 gcggcactgt tatttattct cagtatgatg aaaaaacagg ccagctaatc tttcgctgaa
    27961 catatttgtc ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag
    28021 gcctgataag acgaacagcg tcgcatcagg cacactgcca atgtcggatg cggcgcgagc
    28081 gaccaatccg acttacggca tttctggcgg cgttatgcca aagtgattcc acgcccgcgt
    28141 cgtcgccata cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca aataaggttc
    28201 cagtacatcc tcaatggttt cacgttcttc gccaatggct gccgccaggt tatccagacc
    28261 taccggccca ccaaagaact tatcgattac cgccagcaac aatttgcggt ccatataatc
    28321 gaaaccttca gcatcgacat tcaacatatc cagcgcctga gcagcgatat ctgccgagat
    28381 ggtgccatcg tgcttcactt cggcgaaatc acgcactcga cgcagcagac ggttggcaat
    28441 acgtggcgta ccgcgcgcac gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag
    28501 ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct gataaaactc
    28561 cagacgttgc acaataccaa aacgatcgcg caacggtgat gtcagcgaac ctgcgcgcgt
    28621 ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg ccgccggacc
    28681 ttcaccaatc atgatatcca gttggtaatc ttccattgcc ggatacaaca cctcttccac
    28741 cactggtgaa agacggtgga tctcatcaat aaacagtaca tcgtgtggtt caaggttagt
    28801 gagcattgct gccagatcgc ccgccttttc cagcaccgga ccagaagtcg tgcgtaaatt
    28861 aacgcccatt tcattggcga caatattggc aagcgtagtt ttacccaacc ccggaggacc
    28921 aaaaatcaat agatgatcga gggcatcgcc gcgcagtttc gctgctttga tgaaaatctc
    28981 catctgcgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag ggcggatggc
    29041 gcgatctgcc acatcttccg gcaaagtggt accggcagaa atcagacggt ctgcttcaat
    29101 catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc actgctggcg
    29161 tcagggcgag cgattttgct caccatgcgg cttgcttctt gtggtttata gcccagtgcc
    29221 accagcgcag caaccgcttc ctgttcagca tcgtcggtcg ccgggctggc aggagacgtg
    29281 agtaccaggt cggcggctgg cgtaaagaga tcgccatgca aacctttaaa tcggtctttc
    29341 atttcgacaa tcaagcgttc ggcggttttt ttgccaatac ccggcagttt caccagtgcc
    29401 cccacttctt cacgctcaac ggcattaacg aactgctgcg ctgacattcc ggagaggatc
    29461 gccagcgcca acttcgggcc gacgccgttg gttttgatca actctttgaa caacgtgcgc
    29521 tcttgtttat tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac aaagtgggtg
    29581 aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat cggcatatgc
    29641 acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt ttcaatgatg
    29701 atgcctctga gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag ataatgctgt
    29761 atgataaaaa aatgctggat agatatccag cgaaggatga agaaaacttg cgaggtgtct
    29821 caatgatctg aaaaatgg
//
LOCUS       DAZFRG010000031        29165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFRG010000031 DAZFRG010000000
VERSION     DAZFRG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.031"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..1640)
                     /gene="ampG"
                     /locus_tag="ACHKUC_001092"
     CDS             complement(165..1640)
                     /gene="ampG"
                     /locus_tag="ACHKUC_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO6617380.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNGNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1684..2262)
                     /gene="yajG"
                     /locus_tag="ACHKUC_001093"
     CDS             complement(1684..2262)
                     /gene="yajG"
                     /locus_tag="ACHKUC_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6617381.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2567..2884
                     /gene="bolA"
                     /locus_tag="ACHKUC_001094"
     CDS             2567..2884
                     /gene="bolA"
                     /locus_tag="ACHKUC_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO6617382.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3228..4526
                     /gene="tig"
                     /locus_tag="ACHKUC_001095"
     CDS             3228..4526
                     /gene="tig"
                     /locus_tag="ACHKUC_001095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6617383.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAVQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4772..5395
                     /gene="clpP"
                     /locus_tag="ACHKUC_001096"
     CDS             4772..5395
                     /gene="clpP"
                     /locus_tag="ACHKUC_001096"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO6617384.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5521..6795
                     /gene="clpX"
                     /locus_tag="ACHKUC_001097"
     CDS             5521..6795
                     /gene="clpX"
                     /locus_tag="ACHKUC_001097"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO6617385.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6983..9337
                     /gene="lon"
                     /locus_tag="ACHKUC_001098"
     CDS             6983..9337
                     /gene="lon"
                     /locus_tag="ACHKUC_001098"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO6617386.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9528..9818
                     /gene="hupB"
                     /locus_tag="ACHKUC_001099"
     CDS             9528..9818
                     /gene="hupB"
                     /locus_tag="ACHKUC_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO6617387.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10010..11881
                     /gene="ppiD"
                     /locus_tag="ACHKUC_001100"
     CDS             10010..11881
                     /gene="ppiD"
                     /locus_tag="ACHKUC_001100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6617388.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12032..12403
                     /gene="ybaV"
                     /locus_tag="ACHKUC_001101"
     CDS             12032..12403
                     /gene="ybaV"
                     /locus_tag="ACHKUC_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO6617389.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12509..12907
                     /gene="fadM"
                     /locus_tag="ACHKUC_001102"
     CDS             12509..12907
                     /gene="fadM"
                     /locus_tag="ACHKUC_001102"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO6617390.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLGPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12959..13654)
                     /gene="queC"
                     /locus_tag="ACHKUC_001103"
     CDS             complement(12959..13654)
                     /gene="queC"
                     /locus_tag="ACHKUC_001103"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO6617391.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13719..15419)
                     /gene="ybaE"
                     /locus_tag="ACHKUC_001104"
     CDS             complement(13719..15419)
                     /gene="ybaE"
                     /locus_tag="ACHKUC_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO6617392.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPDWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLEATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15519..16337
                     /gene="cof"
                     /locus_tag="ACHKUC_001105"
     CDS             15519..16337
                     /gene="cof"
                     /locus_tag="ACHKUC_001105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO6617393.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLEAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16490..16948
                     /gene="decR"
                     /locus_tag="ACHKUC_001106"
     CDS             16490..16948
                     /gene="decR"
                     /locus_tag="ACHKUC_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO6617394.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16978..18750
                     /gene="mdlA"
                     /locus_tag="ACHKUC_001107"
     CDS             16978..18750
                     /gene="mdlA"
                     /locus_tag="ACHKUC_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6617395.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18743..20524
                     /gene="mdlB"
                     /locus_tag="ACHKUC_001108"
     CDS             18743..20524
                     /gene="mdlB"
                     /locus_tag="ACHKUC_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6617396.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAVVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20705..21043
                     /gene="glnK"
                     /locus_tag="ACHKUC_001109"
     CDS             20705..21043
                     /gene="glnK"
                     /locus_tag="ACHKUC_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO6617397.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21073..22359
                     /gene="amtB"
                     /locus_tag="ACHKUC_001110"
     CDS             21073..22359
                     /gene="amtB"
                     /locus_tag="ACHKUC_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO6617398.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22408..23268)
                     /gene="tesB"
                     /locus_tag="ACHKUC_001111"
     CDS             complement(22408..23268)
                     /gene="tesB"
                     /locus_tag="ACHKUC_001111"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO6617399.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23486..24058
                     /gene="ybaY"
                     /locus_tag="ACHKUC_001112"
     CDS             23486..24058
                     /gene="ybaY"
                     /locus_tag="ACHKUC_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO6617400.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24089..24400)
                     /locus_tag="ACHKUC_001113"
     CDS             complement(24089..24400)
                     /locus_tag="ACHKUC_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO6617401.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            24563..24659
                     /gene="ffs"
                     /locus_tag="ACHKUC_001114"
     ncRNA           24563..24659
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKUC_001114"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24779..25132
                     /gene="ybaA"
                     /locus_tag="ACHKUC_001115"
     CDS             24779..25132
                     /gene="ybaA"
                     /locus_tag="ACHKUC_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO6617402.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25174..26724)
                     /gene="pdeB"
                     /locus_tag="ACHKUC_001116"
     CDS             complement(25174..26724)
                     /gene="pdeB"
                     /locus_tag="ACHKUC_001116"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO6617403.1"
                     /translation="MRTRHLVSLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHSDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDIQP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26888..27358)
                     /gene="ylaC"
                     /locus_tag="ACHKUC_001117"
     CDS             complement(26888..27358)
                     /gene="ylaC"
                     /locus_tag="ACHKUC_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO6617404.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27474..28025)
                     /gene="maa"
                     /locus_tag="ACHKUC_001118"
     CDS             complement(27474..28025)
                     /gene="maa"
                     /locus_tag="ACHKUC_001118"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO6617405.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28198..28416)
                     /gene="hha"
                     /locus_tag="ACHKUC_001119"
     CDS             complement(28198..28416)
                     /gene="hha"
                     /locus_tag="ACHKUC_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO6617406.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28442..28816)
                     /gene="tomB"
                     /locus_tag="ACHKUC_001120"
     CDS             complement(28442..28816)
                     /gene="tomB"
                     /locus_tag="ACHKUC_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO6617407.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7364 a         6982 c         7849 g         6970 t
ORIGIN      
        1 actcattagc tgaatcgtgt aagaaaaata gaattcataa attgcgtcaa taatttccaa
       61 acgctaagcc gcacaaaaga acaaatatta ataagcgatg ctgatgttta tttagccgta
      121 ataattacgg cgagtgatta ctacagctaa ataatattta cagattacgt cagatgtgtt
      181 tttcgtagcg ccagataatc cagcaaacca ccaagcacaa cgccagaaag ggcgactaaa
      241 accccgactt ccagcagcgc aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatt
      301 gtcaacagca gtaaccacac ggccaacagg ctgatacccg ccgccagtgt ccacatggca
      361 aaggcataac ctgcaggata ttcggtacgg gagataaagt tgccatttac tcgtgtatat
      421 tcaagcgtct ggcggcaaac cagcagcaaa ataagccctg gtacggcagc ggcgacggag
      481 aatagataga atgtcgacca gccgtgtgct tcaacaaacc aacccgccac ggggccgaca
      541 taaactcgcc ctacagcaga aagcgctgag agcagggcaa attgagtagc ggaaaatgac
      601 ttattacaca gcgtcattaa cagcgcgaca aaggctgatg tacccatccc gccacagagg
      661 ttttcgaaaa agacggctgc acccatgctg tagagatgct tatcagtaat cgacagcagc
      721 cagtaaccgg cgttagatgc accttgtaaa atgccgaaaa tcagcagtgc acggaacagc
      781 gacaggcgct gcatcaaaat cccaccgtac aatgcgccaa caatggtcgc cagtaagcca
      841 agcgttttgt taaccacgcc tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac
      901 gtggttgtca ggctcatagc gaatgcgtcg cccagcttat acagcacgat aagaagcaaa
      961 ataagccagg cattattgcg accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc
     1021 agcgttttgg gcacaggaat ggtgtcggtg ggttctggtg caagcaacgt cgcgataata
     1081 caggggatca acagcgccgc catcaaccag tacatgccct gccagcccag ccatttatct
     1141 gccagccaca gggctaagcc gccggaaacc agcatcccta aacggtaacc cagcacgctg
     1201 attgccgcgc ccgcaccacg ttcttctgcc gggagcacat cggttttcca cgcatcgaag
     1261 acaatatcct gggaggcaga gcaaaaagcg atcaccaccg ccagtgccgc catccagcgg
     1321 agctgggtgc ctggttcgag aaaacccatc gccgcaatgg cgactaataa cagaatttgc
     1381 gtggcgagca gccaaccgcg ccgccgcccg aaaaatgggg gcgtgtagcg gtccatcagt
     1441 ggtgaccaga ggaatttaaa aacgtaagcc tggcctacca gagagaagaa accaatggtt
     1501 ttgagatcga tattctcgac cgtcatccag gcctgtaagg tgccggacgt cagggcgagc
     1561 ggtagcccgg aagcaaaacc caggatcagc aatatggctg aacgcggctg ttgaaaaata
     1621 cgtaaatatt gactggacat gggcgtctac aggcccagct tgcaccgggc cagagggcag
     1681 aaattaacgc gcgttctgct tgatgaattc gtggatgctg gtgtcctgag acatatcagc
     1741 gatggtatcg gtcagcacac tgttaaccgc atcggcgata tttttgttgg aggcctggaa
     1801 tgcaccttca acgttgtagc tggcacgata gtttttggtc attttgttgc cattctgcgc
     1861 ggtagcgatg atggcgatat ccgctttggt cgcgatgttg tagcgcacgt tgccctggga
     1921 cacgtcagca tacagttggc taacgatgat ttgcagatta accgggccat tcggaccaac
     1981 catgtatcca cgggcggtca tctgtttttc cagcacttct tgcagcagga aacgcagatc
     2041 gcgggaggcg gtcagggtaa cgatttgatt atcgcgggtg acttttgcca gcgcctgatc
     2101 ggtacgctga tcggcaccat taatgcttac ggtgacgccc atcaggcttg gatcctgctg
     2161 tggcagtgta atcgtcgggg aaacttcgat agttgttggc ggttttgcgc atcctgcaag
     2221 cataaacaga gcaactaacg ggaagaggat ttttttgaac atgttcgggc tctcagagac
     2281 tcttaagcgt gtttggtaaa aattcccgcc atcataacat tgccaacggc gaggggaagt
     2341 gggtaaggca tgtaaattca tcatgttgac gaaataatcg cccctggtaa aagaaacgct
     2401 gatgcgaggc ctgtgtttca atctttaaat cagtaaactt catacgcttg acggaaaaac
     2461 caggacgaaa cctaaatatt tgttgttaag ctgcaatgga aacggtaaaa gcggctagta
     2521 tttaaaggga tggatgacat ctcagcgttg tcggaggaga tatttcatga tgatacgtga
     2581 gcggatagaa gaaaaattaa gggcggcgtt ccaacccgta ttcctcgaag tagtggatga
     2641 aagctatcgt cacaatgtcc cagccggctc tgaaagccat tttaaagttg tgctggtcag
     2701 cgatcgtttt acgggtgaac gttttctgaa tcgtcatcga atgatttaca gtactttagc
     2761 ggaggaactc tctactaccg ttcatgcgct ggctctgcat acttacacta ttaaggagtg
     2821 ggaagggttg caggacaccg tctttgcctc tcctccctgt cgtggagcag gaagcatcgc
     2881 gtaaaaacgc atttgcaact gtcggcgctt ttccagtatg ttgctaaaga ttttatgaaa
     2941 aacggcctgc gggccgtttt gttttgtctg gattttgcgc tttttgccca gcattcagac
     3001 gaaaatcgcc cgggaattgt gaaaaaatac gcgacagcgc gcaataaccg ttctcgactc
     3061 ataaaagtga tgccgctata atgccgcgtc ctatttgaat gctttcggga tgattctggt
     3121 aacagggaat gtgattgatt ataagaacat cccggttccg cgaagccaac aacctgtgct
     3181 tgcggggtaa gagttgaccg agcactgtga ttttttgagg taacaagatg caagtttcag
     3241 ttgaaaccac tcaaggcctt ggccgccgtg taacgattac tatcgctgct gacagcatcg
     3301 agaccgctgt taaaagcgag ctggtcaacg ttgcgaaaaa agtacgtatt gacggcttcc
     3361 gcaaaggcaa agtgccaatg aatatcgttg ctcagcgtta tggcgcgtct gtacgccagg
     3421 acgtcctggg cgacctgatg agccgtaact tcattgacgc catcattaaa gaaaaaatca
     3481 atccggctgg cgcaccgacc tatgttccgg gcgaatacaa gctgggtgaa gacttcacct
     3541 actctgtaga gtttgaagtt tatccggaag ttgaactgca gggtctggaa gcgatcgaag
     3601 ttgaaaaacc gatcgttgaa gtgaccgacg ctgacgttga cggcatgctg gatactctgc
     3661 gtaaacagca ggcaacctgg aaagaaaaag acggcgctgt tgaagcagaa gaccgtgtga
     3721 ccatcgactt caccggttct gtagacggcg aagagttcga aggcggtaaa gcgtctgatt
     3781 tcgtactggc gatgggtcag gggcgcatga tcccgggctt tgaagacggt atcaaaggcc
     3841 acaaagctgg cgaagagttc accatcgacg tgaccttccc ggaagagtac cacgcagaaa
     3901 acctgaaggg taaagcagcg aaattcgcta tcaacctgaa gaaagttgaa gagcgtgaac
     3961 tgccggaact gactgcagaa ttcatcaaac gtttcggcgt tgaagatggt tccgtagaag
     4021 gtctgcgcgc tgaagtgcgt aaaaacatgg agcgcgagct gaagagcgcc atccgtaacc
     4081 gcgttaagtc tcaggcgatc gaaggtctgg taaaagctaa cgacatcgac gtaccggctg
     4141 cgctgatcga cagcgaaatc gacgttctgc gtcgccaggc tgtacagcgt ttcggtggca
     4201 acgaaaaaca agctctggaa ctgccgcgcg aactgttcga agaacaggct aaacgccgcg
     4261 tagttgttgg cctgctgctg ggcgaagtta tccgcaccaa cgagctgaaa gctgacgaag
     4321 agcgcgtgaa aggcctgatc gaagagatgg cttctgcgta cgaagatccg aaagaagtta
     4381 tcgagttcta cagcaaaaac aaagaactga tggacaacat gcgcaatgtt gctctggaag
     4441 aacaggctgt tgaagctgta ctggcgaaag cgaaagtgac tgaaaaagaa accactttca
     4501 acgagctgat gaaccagcag gcgtaattta cgtagcataa cgcgctaaat tcgcacaaag
     4561 gcccgtcacc gccaggtggt gggctttttt ttgtcatgaa ttttgcatgg aaccgtgcga
     4621 aaagcctctt tcggtgttag cgtaacaaca aaagattgtt atgcttgaaa tatggtgatg
     4681 ccgtacccat aacacaggga ctagctgata atccgtccat aaggttacaa tcggtacagc
     4741 aggttttttc aattttatcc aggagacgga aatgtcatac agcggcgaac gagataactt
     4801 tgcaccccat atggcgctgg tgccgatggt cattgaacag acctcacgag gtgagcgctc
     4861 ttttgatatc tattctcgtc tacttaagga acgcgtcatt tttctgactg gccaggttga
     4921 agatcacatg gctaacctga ttgtggcgca gatgctgttc ctggaagcag aaaacccaga
     4981 aaaagatatc tatctgtaca ttaactctcc aggcggggtg attactgccg ggatgtctat
     5041 ctatgacacc atgcagttta tcaagcctga tgtcagcacc atctgtatgg gccaggcggc
     5101 ctcgatgggt gctttcttgc tgactgcagg ggcaaaaggt aaacgtttct gcctgccgaa
     5161 ttcgcgcgtg atgattcacc agccgttggg cggctaccag ggccaggcga ccgatatcga
     5221 aattcatgcc cgtgaaattc tgaaagttaa agggcgcatg aatgaactta tggcgcttca
     5281 tacgggtcaa tcattagaac agattgaacg tgataccgag cgcgatcgct tcctttctgc
     5341 ccctgaagcg gtggaatacg gtctggtcga ttcgattctg acccatcgta attgatgcca
     5401 gaggcgcaac tgtgccgcta tacttatcca gggcggcaca acgctgtaag cggcttgcgc
     5461 ctgagaatgg catttgcgtc gtcgtgtgcg gcacaaagaa caaagaagag gttttgaccc
     5521 atgacagata aacgcaaaga tggctcaggc aaattgctgt attgctcttt ttgcggcaaa
     5581 agccagcatg aagtgcgcaa gctgattgcc ggtccatccg tgtatatctg cgacgaatgt
     5641 gttgatttat gtaacgacat cattcgcgaa gagattaaag aagttgcacc gcatcgtgaa
     5701 cgcagtgcgc taccgacgcc gcatgaaatt cgcaaccacc tggacgatta cgttatcggt
     5761 caggaacagg cgaaaaaagt gctggcggtc gcggtataca accactacaa acgtctgcgc
     5821 aacggagata ccagcaatgg cgtcgagttg ggcaaaagta acattctgct gatcggtccg
     5881 accggttccg gtaaaacgct gctggccgaa acgctggcgc gcctgctgga cgttccgttc
     5941 accatggctg acgcaaccac gctgaccgaa gccggttatg tgggcgaaga cgttgaaaac
     6001 atcattcaga agctgttgca gaagtgcgat tacgacgtac agaaagcgca gcgcgggatt
     6061 gtctacatcg atgaaattga caagatttct cgtaagtcag acaacccgtc cattacccga
     6121 gacgtttccg gtgaaggcgt acagcaggca ctgttgaaac tgatcgaagg tacggtagct
     6181 gctgttccgc cgcaaggtgg gcgtaaacat ccgcaacagg aattcttgca ggttgatacc
     6241 tctaagatcc tgtttatctg tggcggtgcg tttgccggtc tggataaagt gatttcccat
     6301 cgtgtagaaa ccggttccgg cattggtttt ggcgcgacgg taaaagcgaa gtccgacaaa
     6361 gcaagcgaag gcgagctgct ggcgcaggtt gaaccggaag atctgatcaa gtttggtctg
     6421 atccctgagt tcattggtcg tctgccggtt gtcgcaacgt tgaatgaact gagcgaagaa
     6481 gctctgattc agatcctcaa agagccgaaa aacgccctga ccaagcagta tcaggcgctg
     6541 tttaatctgg aaggcgtgga tctggaattc cgtgacgagg cgctggatgc tatcgctaag
     6601 aaagcgatgg cgcgtaaaac cggtgcccgt ggcctgcgtt ctatcgtaga agccgcactg
     6661 ctcgatacca tgtacgatct gccgtccatg gaagatgtcg aaaaagtggt tatcgacgag
     6721 tcggtaattg atggtcaaag caaaccgttg ctgatttatg gtaagccgga agcgcaacag
     6781 gcatctggtg aataattaac cattcccata caattagtta accaaaaagg ggggatttta
     6841 tctccccttt aatttttcct ctattctcgg cgttgaatgt gggggaaaca tccccatata
     6901 ctgacgtaca tgttaataga tggcgtgaag cacagtcgtg tcatctgatt acctggcgga
     6961 aattaaacta agagagagct ctatgaatcc tgagcgttct gaacgcattg aaatccccgt
     7021 attgccgctg cgcgatgtgg tggtttatcc gcacatggtc atccccttat ttgtcgggcg
     7081 ggaaaaatcc atccgttgtc tggaagcggc gatggaccat gataaaaaaa ttatgctggt
     7141 cgcgcagaaa gaagcttcaa cggatgagcc gggtgtaaac gatcttttca ctgtcgggac
     7201 cgtggcctct atattgcaga tgctgaaact gcctgacggc accgtcaaag tgctggtcga
     7261 ggggttacag cgcgcgcgta tttctgcgct ctctgacaat ggcgaacact tttctgcgaa
     7321 ggcggagtat ctggagtcgc cgaccattga tgagcgggaa caggaagtgc tggtgcgtac
     7381 tgcaatcagc cagttcgaag gctacatcaa gctaaacaaa aaaatcccac cagaagtgct
     7441 gacgtcgctg aatagcatcg acgatccggc gcgtctggcg gataccattg ctgcacatat
     7501 gccgctgaaa ctggctgaca aacagtccgt tctggagatg tccgacgtta acgaacgtct
     7561 ggaatatctg atggcaatga tggaatcgga aatcgatctg ctgcaggttg agaaacgcat
     7621 tcgcaaccgc gttaaaaagc agatggagaa atcccagcgt gagtactatc tgaacgagca
     7681 aatgaaagct attcagaaag aactcggtga aatggacgac gcgccggacg aaaacgaagc
     7741 cctgaagcgc aaaatcgacg cggcgaagat gccgaaagag gcaaaagaga aagcggaagc
     7801 agagctgcag aagctgaaaa tgatgtctcc gatgtcggca gaagcgaccg tagtgcgtgg
     7861 atatatcgac tggatggtac aggtgccgtg gaatgcgcgt agcaaggtca aaaaagacct
     7921 gcgtcaggcg caggaaatcc ttgataccga ccattatggt ctggagcgcg tgaaagatcg
     7981 tatccttgag tatcttgcgg ttcaaagccg tgtcaacaaa atcaagggac cgatcctctg
     8041 cctggtaggg ccgccggggg taggtaaaac ctccctgggt cagtccatcg ccaaagccac
     8101 cgggcgtaaa tatgtccgta tggcgctggg cggcgtgcgt gatgaagcgg aaatccgtgg
     8161 tcaccgccgt acttacatcg gttctatgcc gggtaaactg atccagaaaa tggcgaaagt
     8221 gggcgtgaaa aacccgctgt tcctgctcga tgagatcgac aaaatgtctt ctgacatgcg
     8281 tggcgatccg gcctccgcac tgcttgaagt gctggatcca gagcagaacg tagcgttcag
     8341 cgaccactac ctggaagtgg attacgatct cagcgacgta atgtttgtcg ctacgtcgaa
     8401 ctccatgaac attccggcac cgctgctgga tcgtatggaa gtgattcgcc tctccggtta
     8461 taccgaagat gaaaaactga acatcgccaa acgtcacctg ctgccgaagc agattgaacg
     8521 taatgcactg aaaaaaggtg agctgaccgt cgacgatagc gccattatcg gcattattcg
     8581 ttactacacc cgtgaggcgg gcgtgcgtgg tctggagcgt gaaatctcca aactgtgtcg
     8641 caaagcggtt aagcagttac tgctcgataa gtcattaaaa catatcgaaa ttaacggtga
     8701 taacctgcat gactacctcg gtgttcagcg tttcgactat gggcgcgcgg ataacgaaaa
     8761 ccgtgtcggc caggtaaccg gtctggcgtg gacggaagtg ggcggtgact tgctgaccat
     8821 tgaaaccgcg tgcgttccgg ggaaaggtaa actgacctat accggttcgc tcggcgaagt
     8881 gatgcaggag tctattcagg cggcgttaac agtggttcgt gcgcgtgcgg aaaaactggg
     8941 gatcaaccct gatttttacg aaaaacgtga tatccacgtc cacgtaccgg aaggtgcgac
     9001 gccgaaagat ggtccgagtg ccggtattgc catgtgcacc gcgctggttt cttgcctgac
     9061 cggtaacccg gttcgtgccg atgtggcaat gaccggtgag atcactctgc gtggtcaggt
     9121 actgccgatc ggtggtttga aagaaaaact actggcagcg catcgcggcg ggattaaaac
     9181 agtgttaatt ccgttcgaaa ataaacgcga tctggaagag attcctgaca acgtaattgc
     9241 cgatctggac attcatcctg tgaagcgcat tgaggaagtt ctgactctgg cgctacaaaa
     9301 tgaaccgtct ggcatgcagg ttgtgactgc aaaatagtga cctcgcgcaa aatgcactaa
     9361 taaaaacagg gctggcaggc taattcgggc ttgccagcct ttttttgtct cgctaagtta
     9421 gatggcggat caggcttgcc cttattaagg ggtgttgtaa ggggatggct ggcctgatat
     9481 aactgctgcg cgttcgtacc ttgaaggatt caagtgcgat ataaattata aagaggaaga
     9541 gaagagtgaa taaatctcaa ttgatcgaca agattgctgc aggggctgat atctctaaag
     9601 ctgcggctgg ccgtgcgtta gatgctatta ttgcttccgt aactgaatct ctgaaagaag
     9661 gggatgatgt agcactggta ggttttggta cttttgccgt taaagagcgt gctgcccgta
     9721 ctggccgcaa cccgcagacc ggtaaagaga tcaccatcgc tgctgctaaa gtaccgagct
     9781 tccgtgcagg taaagcactg aaagacgcgg taaactaagc gttgtcccca gtggggatgt
     9841 gacgaagttc aagggcgcat ctactgatgt gcctttttta tttgtattcg gtgactttct
     9901 gcgtcttgtg ggctgacaat tgcccccgtt tcttgtcaca ataggccttt gcgcgcatcg
     9961 atacgttgcg tgaggtacac agtcatctac agcggagtgt tgttacacca tgatggacag
    10021 cttacgcacg gctgcaaaca gtctcgtgct caagattatt ttcggtatca ttatcgtgtc
    10081 gttcatattg accggcgtga gtggttacct gattggcgga ggcaataact acgccgcaaa
    10141 agtgaatgac caggaaatca gccgtgggca attcgagaac gccttcaaca gcgagcgtaa
    10201 tcgcatgcag caacagctgg gcgatcaata ctccgagctg gcagcgaacg aaggctatat
    10261 gaaaaccctg cgtcaacagg tgctgaatcg tctgatcgac gaggcgcttc tggatcagta
    10321 cgctcgtgag ctgaaactgg gtatcagcga tgagcaggtt aaacaggcga ttttcgcgac
    10381 cccagccttc caggttgacg gcaaatttga taacagccgc tataacggta tcctcaacca
    10441 gatggggatg accgccgatc agtacgccca ggcgctgcgt aaccagctca ctacccaaca
    10501 gctgattaac ggcgttgccg gtaccgattt tatgctgaaa ggtgaaaccg acgagctggc
    10561 ggcactggta gctcaacaac gcgtggtgcg tgaagcgact atcgatgtta acgcgctggc
    10621 ggcgaagcag cctgtgaccg aacaggaaat tgccagctac tacgaacaaa acaaaaacaa
    10681 tttcatgacg ccggaacaat tccgcgtgag ttacatcaag ctggatgccg cgacgatgca
    10741 gcaaccggtt agcgatgcgg atatccagag ctactacgat cagcatcagg atcaattcac
    10801 ccagccgcag cgtacccgtt acagcatcat ccagaccaaa actgaagatg aagcgaaagc
    10861 ggtacttgat gagctgaata agggcggtga ttttgctgca ttagccaaag aaaaatctgc
    10921 cgatattatc tctgcccgca acggcggtga tatgggttgg ttagaagatg ccactatccc
    10981 ggacgaactg aaaaatgcgg gtctgaaaga aaaaggccaa ctgtctggtg ttatcaaatc
    11041 ttcggtcggt ttcctgattg tgcgtctgga cgacattcag ccagcgaaag tgaaatcgtt
    11101 agacgaagta cgtgacgaca ttgcggcgaa agtgaaacac gaaaaagccc tcgatgcgta
    11161 ctacgcgctg cagcagaaag tgagcgatgc ggcaagcaac gacaccgagt ctctggccgg
    11221 tgcagagcaa gctgccggcg ttaaagccac tcagacgggt tggttcagca aagataacct
    11281 gccggaagag ttgaacttca agccagttgc cgacgctatc ttcaacggcg gtctggtagg
    11341 tgaaaacggc gcgccgggca tcaactctga catcatcacc gtagacggcg accgcgcatt
    11401 cgtgctgcgc atcagcgagc acaaaccgga agcggtgaaa ccgttggcag atgttcagga
    11461 acaagttaag gcactggttc agcacaacaa agctgaacaa caggcgaaag tggatgctga
    11521 gaaactgctg gttgatttga aagcaggcaa aggtgcggaa gctatgcagg ctgccggtct
    11581 gaaatttggc gagccgaaaa ccttaagtcg ttccggtcgt gacccgatta gccaggctgc
    11641 gtttgcactg ccgctgccag cgaaagacaa accgagctac ggtatggcga ccgatatgca
    11701 aggtaatgta gttctgctgg cgctggatga agtgaagcaa ggttcaatgc cggaagatca
    11761 gaaaaaagcg atggtgcagg gtatcaccca gaacaacgca caaatcgtct ttgaagctct
    11821 gatgagtaac ctgcgtaaag aggcgaaaat caaaattggc gatgcgctgg aacagcaata
    11881 atcctgaagc cgcctcgcaa aaaaatgctt tgcagctgta acaagaaaag gtcgctttcg
    11941 cggccttttc catttccgaa cattgccatt tgtttactgt tttcactgcc gttaaggtga
    12001 ttccactgtt aacaaacaag gagaaaacag tatgaaacac ggaattaaag cactgctcat
    12061 taccctgtcc ctggcctgtg ccggaatgtc tcatagtgct ctggcggcag ctcctgcggc
    12121 aaaaccgaca acggtagaaa ccaaagcgga agctcctgca gcacaaagta aagcagcagt
    12181 tccggcgaaa gccagtgacg aagaaggcac tcgggtcagc attaataatg ccagcgcgga
    12241 agagttagcc cgcgcgatga atggcgttgg cctgaagaaa gcgcaggcga ttgtcagtta
    12301 tcgcgaagag tacggtccgt ttaaaacggt ggaggatcta aagcaggtgc cggggatggg
    12361 caattcgctg gtggaacgta atctggcggt attaaccctg taattaattt gcatagtggc
    12421 aattttttgc cagactgaag aggtcatacc agttatgacc tctgtactta taacagttct
    12481 tataacaaca acgtaaggtt attgcgctat gcaaacacaa atcaaagttc gtggatatca
    12541 tctcgacgtt taccagcacg taaacaacgc ccgctacctt gaatttctcg aagaagcccg
    12601 ctggcatggg ttggaaaata gcgacagttt tcactggatg acggcccata acatcgcttt
    12661 cgtcgtggtc aatatcaata ttaactatcg ccgcccggcg gtattaagcg acctgttaac
    12721 cattaccagt cagttgcagc aattaaacgg taaaagtggc atcttaagcc aggtcattac
    12781 actggggccg gaaggacagg tggtagcgga tgcgcttatt acgtttgttt gtattgatct
    12841 taaaacgcag aaagcattag ctctggaagg ggaattgcgc gaaaagctgg agcagatggt
    12901 taagtaaacg ttttgtggtg ccggatgctc aagccgcatc cggcgacacc cggaataatt
    12961 acttcaaccc ggttttctgc ttcatcgctg ccatcaccgt cggtttatcg gccagataat
    13021 gattcaaacc gttggcacgt aaattacacg ccgcacaatg accgcaaccg tcgcctttaa
    13081 tgccgttata acaggtcagc gtttcgttac ggactaaatc cagcttgccg taataatctg
    13141 ccagcgccca ggtttccgct ttatcaatcc acatcagcgg cgtttcaaaa cgaatatctt
    13201 tcgccatgcc caaactgacg gcatggttta gtgctttcac aaactcatcg cggcaatccg
    13261 ggtagccgga gaaatccgtt tcgcagacgc cggtaattac ggcttctgct tttacctgat
    13321 acgcatatat tgccgccagt gtcaggaaca aaatattacg ccctgggaca aacgtattcg
    13381 ggataccatc ggcttcaggt tcataatcag gcaccggaat gctgtcacgc gtcaggctgc
    13441 tgaccgccag ctcgttgagc agcgttacat caagcacctt atgcgcgcgt gcccccagtt
    13501 tcagcgccag ttcgcgtgcc acgtcgattt ctgcgcgatg gcgttgaccg taatcgaacg
    13561 tcacgcaatg gacttcatca tattgttgta atgcctgcac cagacaggtg gtggaatcct
    13621 ggcctccact gaacacaacg acagcacgtt tcatagataa tcctgcttga acaataaaaa
    13681 cgttatggta acgcctgcga ttaacccgga ccagctattc attgcgacgg cgcgggaagc
    13741 caggcttcgg taaattcaaa ccagccgcgc ggtgtcagtc gcacaccgtt cacgccggga
    13801 ggggcgctaa tgcgatagtg atagttgaac agcggcgtca gcgtcgcatc tgccattaac
    13861 tggctaaaaa ccgctttcag ggcattaaat cggttttcct catcaggcat tacttgcacc
    13921 gcgtccagtg tcgattgcaa atgtgcatat gctggagcgt cgaaaacatg tggccacagt
    13981 ggatcgcaac gcagccattg ctccagggta tattccggtg cttcgccaat taatctgtcg
    14041 cccatcatga ggtctgcgtg tgccagtagg gtcgtgtcgt cccagttttt tgcgttatga
    14101 aaaataattg tgagttcaca gccttccgct gccagtgtcg cctctaggcg ttctgccatg
    14161 gtatgaagtt ctatcggtag gtgataaacc agcgtcaagg ttttcggtag tttaacttca
    14221 tccggtactt gccattgtgg aatagtccag tctggcagta atgcatgact ggcggtgatc
    14281 aggttttctc cgacttctaa cgtttgtaat aaaccggatt gatgaataat ggagatcact
    14341 tttcgcgcct gccagaggga cagtcgggga cttttgcgca acgttaaata gcaaaaacct
    14401 aaactgatgc cactactgac ctggctgacc cgttgcagct cctcaggttt gccgatagtg
    14461 atttgcacgg gatgccgaca actggttccc aaatcttttt cgaaaagcgg aggagttatc
    14521 cagtactcaa ccgctttaag cagcggatga cgtaaatggt aataatcatg gctttccagg
    14581 cgcaccagtt ccgctgtgaa ttgtgttaag cgaaaaggac cagtgccgat cagggggaat
    14641 tgcggatgcg ccagatggct gcaatagctc gccagccggt gcgcaagcca gtaatcgggg
    14701 cgatgtaaaa agaaggtcag acactgcgga tgggtgactt caatacgctt cacgctaata
    14761 aataattgat ccagtgctgg cagttgtaac agcatcaata accgctggtg taagtgggag
    14821 gcttttactg catcgccgtt atgccagtgt aaggttgagc gaagataaaa atcccagcgt
    14881 aacccgtcag tagaggtttc ccagtgatgc gctaaatcgc caatcgggcg ctgggtatta
    14941 ttatcgaagc gggtcaggcc ggaaaatatc tgtccggcga gatgctgctc ggcacgaccg
    15001 ggcaaaaagc ccggttgtag cggttcgagc gggcgatagt agggaatacg caacgtgggc
    15061 gtatcgtttt gccattgtcc gcccataaac ggctgtaaca gagtgcgcag ctcacctggg
    15121 gccagttgcg ccagctccag cacatcttgc tgctttccgg tttccagcgc ctgttccatc
    15181 atcgcattgc gtagcgattc tggcgtgacc agaaagcgta attgtccgcg ctttccgcgt
    15241 cctgactgcg cctgccactc cagccatccc gcctcctgtg cctgacgcaa cagcgtacga
    15301 acatggcgtt cgctgcaaaa acagcgttcg gccagttcgc tgacggtgac ggtttgcggc
    15361 tttccggcgg aaggttgcca cagacgttga tactggttaa gacggttgag cagtcgcata
    15421 taaacccgga acaatattat ttaactattc actattactt ccgtatatat caggtgatac
    15481 tcaatcacca ttaaccgtgt cacagagtgg agaaagaaat ggctcgtctg gcagcatttg
    15541 atatggatgg cactttattg atgcctgacc atcatttagg tgagaaaacc ctctctactt
    15601 tggcgcgact gcgtgaacgc gacattaccc tcacttttgc cacggggcgt catgcgctgg
    15661 agatgcagca tattctcggg gcgctatcgc tggaggcata tttgattact ggcaacggaa
    15721 cgcgcgtgca ttctctggaa ggtgaacttt tacatcgtga tgatttacct gcggatgtcg
    15781 cggagctggt gctgtatcag caatgggata cccgagcaag catgcatatc ttcaatgacg
    15841 atggttggtt taccgggaaa gagatccctg cgttgttgca ggcatttgtc tatagcggtt
    15901 ttcgttatca gataatcgat gtcaaaaaaa tgccactcgg cagcgtcacc aagatctgct
    15961 tctgtggcga tcacgacgat cttacacgct tgcagatcca gctatacgaa gcattaggcg
    16021 agcgtgcaca tttatgtttt tccgccacgg attgcctcga agtgctgccg gtgggctgca
    16081 ataaaggcgc tgcattgacg gtgctgaccc aacatttagg tttatcgtta cgcgattgca
    16141 tggcctttgg tgatgcgatg aacgatcgcg aaatgttagg cagcgtcggt agcggattta
    16201 ttatgggcaa tgcgatgccg caactgcgcg cggagctccc gcatttaccg gtgattggac
    16261 attgccgaaa tcaggctgtc tctcactatt tgacgcactg gctggactat ccacatctac
    16321 cttattcccc cgaataacga gatcccttcc agcaccgggc aattgcccgg ttttttttgc
    16381 gttgaatttg tcattttgtg ccgtggtgct taaaccgcac agaataaatt gtcgcgattt
    16441 cacctttaaa atagaattaa aagagaaaaa aattctctgt ggaagggcta tgttagataa
    16501 aattgaccgt aagctgctgg ccttgctaca gcaggattgc accctctctt tgcaggcact
    16561 ggctgaagcc gttaatctga caaccacccc ctgctggaag cgtctgaaac ggctggagga
    16621 cgacggtatc cttatcggca aagtcgccct gctggatccg gaaaaaatag gcctcggcct
    16681 gaccgccttt gtgctgataa aaacgcaaca tcacagcagc gaatggtatt gccgctttgt
    16741 cacggtggtt accgaaatgc cagaagtgct ggggttctgg cgcatggctg gtgaatacga
    16801 ttatctgatg cgcgtccagg ttgccgacat gaaacgctac gacgagtttt ataagcgtct
    16861 ggtaaacagt gtaccggggc tgtcggacgt cacttccagc ttcgcgatgg aacagattaa
    16921 atacaccact tctttaccca tcgaataaat atccagaatc aagtcaggac acaacgcgtg
    16981 cgattatttg ctcaattaag ctggtatttc cgtcgggaat ggcgtcgcta tctcggggct
    17041 gtcgccttgc ttgtcattat cgcgatgctg caactggttc cgccaaaagt ggttggtatt
    17101 gttgttgatg gcgtgacaga acaacacttt actaccgggc agatcctgat gtggatcgcc
    17161 accatggtgc tgattgccgt tgtggtttat ctcctgcgtt acgtctggcg cgtattgctg
    17221 tttggtgcgt cttatcaact ggctgttgaa ctgcgtgaag attattaccg ccagctaagc
    17281 cggcagcatc ctgagtttta cctgcgtcat cgcacgggtg acctcatggc tcgtgcgacc
    17341 aatgacgtcg atcgcgtcgt gtttgccgcc ggagaagggg tgctgacgct ggtggattca
    17401 ctggtaatgg gctgcgctgt gttgattatg atgtctacgc aaattagctg gcagttgacc
    17461 ttatttgccc tgttgccgat gccagtgatg gcgatcatga tcaagcgcaa cggcgatgcc
    17521 ttgcatgaac gctttaagct ggcacaggcg gcgttttcca gtcttaatga ccgcacccag
    17581 gaaagcctca ccagtatccg catgatcaaa gcctttggtc tggaagatcg tcagtcggcg
    17641 ttatttgccg cggatgccga agataccggc aaaaaaaaca tgcgggtggc gcgtattgat
    17701 gctcgtttcg acccgaccat ctatatcgcg attggtatgg cgaacttgct ggcgattggc
    17761 ggcggtagtt ggatggtggt acagggcagt ttaacgctgg gccagctcac cagttttatg
    17821 atgtatttag gtctgatgat ttggccaatg ctggcgctgg catggatgtt taacattgtg
    17881 gaacgtggca gcgcggcgta tagccgaatt cgtaccatgc tggccgaagc gccagtcgtt
    17941 attgatggta gtgataaggt gccagaaggt cgtggtgaac tggatgtgaa tattcgccag
    18001 tttacgtatc cgcagactga ccatcctgcg ctggaaaacg tcaatttcgc cctgaaaccc
    18061 ggtcagatgc tgggtatctg cgggccgact ggttccggca aaagtaccct gttgtcgctc
    18121 attcagcgtc atttcgacgt cagcgagggt gatatccgtt ttcatgatat tcctctgacg
    18181 aagttacaac tcgatagctg gcgtagccgt ctggcggtgg tcagccagac accattcctt
    18241 ttttccgaca ccgtggcgaa caacatcgcg ttgggttgcc cgaatgccac ccagcaagag
    18301 attgaacacg tagcgcggtt agccagcgta catgacgata ttttgcgtct gccgcaaggt
    18361 tacgacacag aagtgggcga gcgcggcgtg atgctttctg gcggacaaaa acagcgtatc
    18421 tccattgctc gagcgttatt agtcaacgcg gaaatcctca tccttgacga tgcgctttca
    18481 gcggtggacg gacgcacaga gcaccagatc ctgcataacc tgcgtcagtg ggggcaggga
    18541 agaacggtaa tcatcagtgc ccatcgcctt tctgcactga cggaagccag tgaaattatt
    18601 gtgatgcagc acgggcatat cgcccagcgt ggcaatcatg atgtgctggt acaacaaagc
    18661 ggctggtatc gcgatatgta tcgctatcaa caactggagg cagcgctcga cgacgccccg
    18721 gaaattcgcg aggaggctgt cgatgcgtag ttttagccaa ctgtggccga ctctcaagcg
    18781 tctgttagcg tacggttcac cgtggcgtaa accgttgggg attgcggtcc tgatgatgtg
    18841 ggttgcggcg gcggcagaag tcagtgggcc gctgcttatc agctatttta tcgacaatat
    18901 ggtagcgaaa aataacctgc cgttgaaagt ggttgcaggg ctggcggcgg cgtatgttgg
    18961 gctgcaactg tttgcagccg ggctacatta cgcgcagtcg ctgctgttta atcgggcggc
    19021 agtaggcgta gtgcaacagt tgcgtaccga cgtgatggat gctgcgttac gccagccatt
    19081 aagcgagttt gatacccaac ccgtcgggca ggtgatttcc cgcgtcacta atgacactga
    19141 agtgatccgc gatctctacg ttaccgtagt ggcaactgtc ctgcgtagcg ccgcactggt
    19201 gggcgcgatg ctggtggcga tgttcagcct cgactggcga atggcgctgg tggcgataat
    19261 gattttcccg gtggtgctgg tggtaatggt gatatatcag cgttacagca cgccgattgt
    19321 ccgtcgtgtg cgcgcttatc tggcggatat caacgacggc tttaacgaaa tcatcaatgg
    19381 catgagcgtt atccagcagt tccgtcagca ggcgcgattt ggcgaacgta tgggtgaggc
    19441 cagtcgttca cactatatgg cgaggatgca aaccctgcgc ctcgacggtt ttctgctgcg
    19501 tccgctgctg agtctgtttt catcgctcat tctttgtggc ttgttgatgc tgtttggctt
    19561 ctccgccagc ggcaccattg aagtgggcgt gttgtatgcg ttcatcagtt atttggggcg
    19621 acttaacgaa cccttaatcg aactgaccac gcagcaggcg atgctgcaac aggctgttgt
    19681 tgctggtgag cgcgtttttg agctgatgga cggaccgcgc cagcaatatg gcaatgatga
    19741 tcgcccgttg cagagcggca ccatcgaagt cgataacgtg tcatttgctt atcgcgatga
    19801 caatctggtg ctaaagaaca ttaatctctc tgtgccttcg cgcaactttg tggcgctggt
    19861 cgggcatacc ggcagtggca aaagcaccct cgccagttta ttgatgggct attacccgct
    19921 aacggaaggt gagattcgcc ttgatggtcg tccattaagt acgctaagtc acagcgcgct
    19981 gcgccagggc gtggcaatgg tgcagcaaga tccggtggtg ctggcggata ccttcctcgc
    20041 caacgtgacg ctgggacgtg atatctctga agaacgcgtc tggcaggcgc tggaaaccgt
    20101 gcagctggcg gagctggcgc gtagcatgag tgatggtatt tacacgccgc ttggcgagca
    20161 ggggaataat ctctcagtcg ggcaaaagca actgctggca ctggcgcgcg tgctggtgga
    20221 gacgccgcaa atcctgatcc ttgatgaggc aaccgctagc attgactccg ggactgaaca
    20281 ggcgattcaa catgctctgg cggtggtgcg tgaacatact acgcttgtgg tgattgctca
    20341 ccgcttatcg actattgttg atgccgacac cattctggtg cttcatcgtg ggcaagccgt
    20401 ggagcagggc actcaccagc aactgctggc ggcccaggga cgatactggc agatgtatca
    20461 actgcaactt gcgggcgaag agctggcagc cagcgtgcgt gaagaggaat cattgagcgc
    20521 ctgaatagcg caatatttca tcgttggtgc aaaaatgtaa cgcactgtgc actgtcatag
    20581 tgcgttttca ttttcaatct tcttaacttc ctgctctctt tctcgttttt catttttggc
    20641 acaccgcttg caataccttc ttcgtgtagc agaaccatta ccgaattctg accggagggg
    20701 atctatgaag ctggtgaccg tgattatcaa accattcaaa cttgaagacg ttcgtgaagc
    20761 gttatcttcc attggtattc agggcctgac cgtcaccgaa gtgaaaggtt tcgggcgtca
    20821 gaaagggcat gccgagctgt accggggggc ggaatacagc gtcaacttcc tgccaaaagt
    20881 aaaaattgat gtggcgattg ctgatgacca actcgatgaa gtgatcgata tcgtcagcaa
    20941 agcggcttac accggaaaaa ttggcgacgg caaaatcttc gtcgctgaat tgcaacgcgt
    21001 cattcgtatt cgtaccggcg aagccgacga agcggcgctg taatctctgg cacacagcaa
    21061 caggaacgaa aaatgaagat agcaacgata aaaactgggc ttacttcact ggcgatgctg
    21121 ccgggactgg taatggctgc acctgcggtg gccgataaag ccgacaatgc gtttatgatg
    21181 atttgtactg cgctggtgct gtttatgacc gttccgggga ttgccctgtt ttatggaggg
    21241 ctgattcgtg gcaaaaacgt gctgtcgatg ctgacgcagg tgaccgtgac gtttgcactg
    21301 gtctgtattc tctgggtggt ttacggctac tcgctggcgt ttggcgaagg caacaacttc
    21361 ttcggcaaca ttaacgggtt gatgctgaaa aacattgaac tgacggcggt gatgggcagc
    21421 atttatcagt atatccacgt ggcgtttcag ggatcgtttg cctgcattac cgtaggcttg
    21481 atagttgggg cgctggcgga acgaatccgc ttctcagctg tgttgatttt tgtggtggta
    21541 tggctgacgc tctcctacat tccgattgcg catatggtct ggggcggcgg tttgctggct
    21601 tctcacgggg cactggattt cgcgggtggc acggtggtgc acattaacgc cgcaatcgcc
    21661 ggtctggtgg gcgcgtatct gataggcaaa cgcgtgggct tcggtaaaga ggcgtttaaa
    21721 ccgcacaacc tgccgatggt cttcaccggg actgctattc tctatatcgg ttggtttggc
    21781 tttaacgccg ggtcagcggg cacggcgaat gaaatcgcgg cactggcatt tgtgaatact
    21841 gtggtcgcaa cggcggcagc aattcttggc tggatcttcg gtgaatgggc gctgcgcggt
    21901 aagccttcac tgctgggagc gtgttctggc gcgattgccg gtctggtcgg cgtgacgcca
    21961 gcgtgtggct acattggtgt tggcggcgcg ttgattatcg gcgtggtagc tggtctggcg
    22021 ggcttgtggg gcgttaccat gctcaaacgc ttgctgcggg tggatgatcc ctgcgatgtc
    22081 ttcggtgtgc acggtgtttg tggcattgtc ggctgtatca tgaccgggat ttttgccgcc
    22141 agttcgctgg gcggcgtggg cttcgctgaa ggtgtgacga tgggccatca gttgctggta
    22201 cagctggaaa gcatcgccat tacgattgtc tggtctggtg tggtggcatt tatcggctac
    22261 aaactggcgg atttgacggt tggtctgcgt gtaccggaag agcaggagcg agaagggctg
    22321 gacgtcaaca gccacggcga gaatgcctat aacgcgtaac aagcactgca aaaaacagcc
    22381 ggacggtttt cacctccggc tattttttta attgtgatta cgcatcaccc cttcctgaac
    22441 ggtcgaggca accagcacgc cgtcctgggt ataaaactca ccgcgcacaa agccgcgtgc
    22501 gctggacgcc gaggtgcttt ccacgctata cagcagccat tcattcaaat taaacgggcg
    22561 atggaaccac atggaatggt caatggtggc aatctgaatc cccggttcga gaaaaccgat
    22621 gccgtgcggc tgtagagcta ccggcaggaa attaagatca gaagcataac cgagcagata
    22681 ctgatgaacg cgcagatcat ccggcacgct accatttgcg cggatccaca cctgacgatg
    22741 cggttctgcg acgtgacctt tcagtgggtt atgaaactcc accggacgga cttccagcgg
    22801 acgatcgcag atgaatttat ctttcagcac tggcggcagc aggtgcgcca gcgattgggc
    22861 gatttgcgtt tctgaaggca ggccatcagg cgctggcgcg gacggcattg ttttttgatg
    22921 ttcgaaaccc gcttctggtg cctggaaaga ggcagtcata taaaaaatcg gtttgccgtt
    22981 ttgaatagca gcaacccggc gggcgctgaa gctgttaccg tcacgcagcg tttcgacatc
    23041 ataaataatc ggcttcttac tatcgccagg gcgaagaaag tagctgtgaa aagaatgtac
    23101 cagccgctcg tcagggacgg tctcttttgc agcatacaag gcctgaccca cgacctggcc
    23161 gccaaatacc tggcgtaaac ctaaatcttc actctggccg cgaaagagtc cttcctcaat
    23221 tttttccaga tttaacaatg tcagtaaatt ttttagcgcc tgactcatat aactctccag
    23281 taacaaagct gccgcagcaa gccaaagtga gttgagtata acgcaaattt gccactggtc
    23341 cgatgggtgc aatggtctga attacgggct aattacaggc agaaatgcgt gatgtgtgcc
    23401 acacttgttg acgttactat tttgttaacc actcttccgg cgaggaaagt tagcccgctg
    23461 gtgcattgat aataaggaga agtgaatgaa actcgtgcac atggccagtg gtttagcggt
    23521 tgcgattgcg ttggcggctt gcgcagataa aagcgcggat attcagacgc cagcaccggc
    23581 tgcaaatacg tctatttcag caacacaaca acctgctatc cagcaaccga atgtctccgg
    23641 taccgtttgg atccgtcaga aagtcgcact gccgcctgat gccgtgctga ccgtgacact
    23701 ttctgacgcg tcgttagccg atgcgccgtc gaaagtgctg gcgcagaaag cggtgcgtac
    23761 cgaaggtaaa cagtcaccat tcagctttgt tctgccattt aacccggcag atgttcagcc
    23821 gaacgcgcgt attctgttga gtgcggcgat taccgtgaat gacaaactgg tatttattac
    23881 cgataccgtt cagccggtga tcaaccaggg cggaactaaa gccgacctga cattggtgcc
    23941 ggtgcagcaa accgccgtgc cggttcaggc tagcggtggc gcaacgacta ccgtaccttc
    24001 aacttcacca acccaggtga atccgtcttc ggcagttcca gctccaacgc aatattaagc
    24061 gaagttaacc ctctccgccc ggagagggtt agtagttcca gcgataaagc tgcaagtcga
    24121 tttgcccgct tcccgacacc atcacacctt ctgccagtaa tgcctgtcgc tgacgctgta
    24181 aatccggtcc ggtcagtgaa atggtgccgt ggcgattaac cacccggtgc cagggcaagg
    24241 tgctgccttc ggggagacgc tttaacacac cgcctacctg gcgcgcggcg cgcggcgatc
    24301 ccgccagttt cgccacatca ccgtaagtgg tgacatagcc ttcgggaata gcggctacga
    24361 tttgccagac gcgttgggga aatgaatctt ctttttccat cttttcttcc tgaggtaatt
    24421 tttcagcata atctggaaaa acgcccgagt gaagccgcat tgcgcaagaa accagcatct
    24481 ggcacgcaat gggttgcaat tagccggggc agcagtgata atgcgcctgc gcgttggttc
    24541 tcaacgctct caatgggggc tctgttggtt ctcccgcaac gctactctgt ttaccaggtc
    24601 aggtccggaa ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc
    24661 ccccacccat ttctgcctcc caccgtttcg tcaaaaaatc ccaacatggc taaactttaa
    24721 ccacaactga cgtcgcaaga attgtctggc tgcgcagtac gcttcggagg tatgtctgat
    24781 gaagtatgtt gatggttttg ttgttgccgt tcctgccgat aaaaaggatg cctatcggga
    24841 aatggccgct aaggccgcgc cattatttaa agagttcggt gcgcttcgta ttgtcgaatg
    24901 ctgggccagc gatgtaccgg atggtaaagt gaccgatttt cgtatggcgg tgaaagcgga
    24961 agagaatgaa gaggttgtct ttagctggat tgaataccct tcaaaagagg tccgcgacgc
    25021 tgctaatcaa aagatgatgt cggacccacg gatgaaagag ttcggcgagt ccatgccgtt
    25081 tgacggcaag cgaatgatct atggtggatt cgaatcaatc atcgacgagt aggtagcgtg
    25141 acgggctgtg cggcgtgccg tcagccctaa aattcacaaa tgttgctcgg cccagcgtaa
    25201 gaaatcctct ttcggtaatg ccttgctgta gagccagccc tgaccgtagt gcacgccatg
    25261 ctggcgtagc cactcttctt gtttactggt ttcgattccc tccgcaacca ttttcagttt
    25321 cagtgttttt gccatttcga tgatatgcgg cgtgacattt ttatattcca gcgcatcaac
    25381 gaaagattta tcgatcttca gaatgtcgac atccagatcc tgtaaatagc ttaaacttga
    25441 ataccctgta ccaaaatcat cgagataaat ttcatggccc gcctcccggt agcgagaaat
    25501 tatcggggcg ctggttttcg gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg
    25561 tctgggatta acctgatagt gattgatcat ttcacgcagc aattgcggga ttttttccga
    25621 ggtgagcacg gtggattcaa gattgatcga aatatgctgc tgaggatgct gacgcagcca
    25681 gtcgcccata tcttcaaaga cgcttcttat aatcagtagc gtcaatggct cagaaaggcc
    25741 cgtttgctgt gccagcggaa taaaactatc tggtgacaac caactaccgt ctgtctgcgg
    25801 ccagcgcgcc agtgcctcag caccgacaat tttgccattg gctaaggaga caatcggctg
    25861 atagtgcacg caaatatcac gattttcgat agcatcctgc agccgatgat gcggtgactg
    25921 gatacggcgc aaaatacgca gcacaaacat cgctgccagc aggccaatca ccaacccgag
    25981 cggtaaccaa ataaagactt gccgatgcca gcctttctgc aacattttcg ttgaagccca
    26041 ggtgatgatc gaaatattca tctccggtaa gggctggata tcgtaaatga ttccattatt
    26101 ttcgatatgc tcaccgggtg ttttttgtag cctggtaata attccctgag caagttcatc
    26161 gctgctggtt atgacaacgt tatgggcatt gccaataatg gcggcatcaa tttgccatga
    26221 gctataggga ataacatcaa taaaggaggc agggtcgatc atgacgacat aatgtgccgt
    26281 tcccatggcg accatgtaac ggataatgcc taaatcgcta tgcgatgtta accagacacg
    26341 ataaccatct ttcgaaattt tacctggctc ggggaaggta tcgggcgggc tttcatgctc
    26401 cagagacgaa cactggggaa cgttgttatc gatataaacc acttcctgaa tatagcgata
    26461 actgtaagat acccgacgca tttccatgag atgggcttcg ctacaggcag cgccttgcca
    26521 tctttccagc tcttgcagcg catctttccc ttgtgtcgcc accttattgg cccgaatagc
    26581 gacgcgggag gaatacgtat tcagctcttc aataaatgat gtttctacct gctgatgggc
    26641 cagccagatg cttaagccga caggcagcaa tactgaaaga atcagaactc ccgaaataag
    26701 gctgaccaga tgtcgtgttc tcaccagcat atccttatta acacagtgta gggaaagtat
    26761 atctgattgt ggaaaaaggc tggcaggaga taaggaagta gggctggtat ttcgtgaagt
    26821 gatgggtgga aacgatcgtt gtcatttgac caggcgtaga gctttacgcc tggtatgtaa
    26881 ccctaactta agatgttgat tcggcgcgag cgagggtaaa aatatcgtaa aaagatagtt
    26941 cacctttacg gacgatcatt ttttgcagtt gttctttatg aacatccgcg acacgcggag
    27001 agttcaggat ggcttcaacc agtgcggcag gtacaaagcg cgtgttgtac cattcgccgt
    27061 tatagcaaac gcggaaatcc agcacgtcga tatcttcata gcggtaacgt gggtagacat
    27121 cgaaaaagaa gaaaccatta agcaggataa ataatacaac cagtagccag acagagcctg
    27181 aaagcgtttc ggattgcaac atcaccgcca gggtagcaaa aaaagcaacg tacataccga
    27241 taaacagccc cggatgttta cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt
    27301 tttcgcgggt attcagagac tcaatcgttt ctgtcagcag gcgttgtatt tcggtcatcg
    27361 tttgccttcg tgatatctgc aactttccag ggaacaccct attttaacgt gggaatagta
    27421 gaggggaagt aacagatttg ccacgatttt gcataacagt tgagaaaagc tggttacaat
    27481 tttttaatga ttctggctgg attaccgccc acgacaacgt tgtccgggac atcttttgtg
    27541 acaactgcac ctgaggctac cacgacgtta tcaccaatgg tcacaccagg gttaatgact
    27601 gcacgtccgc caatccagac gttattaccg atggtgacgg gtttccccag ttcagcaccg
    27661 ctattacgtg ctacagggtc gatgggatgt gttgccgtgt agatatgaac gcctggtgcc
    27721 aacatacagt tatcaccaat gcgaataggg cagacatcaa gcatcacgca atcgaagttg
    27781 gcgaaaaaat tattgccgag aaaaatgtta tagccatagt cacagcgaaa cgttggctca
    27841 atataagcct ctgtcacctg accgaataaa tcagcaagaa tttgctggcg taatgtgtgc
    27901 tcttccgcca gggaatgatt gtatcggtga ataagccgac gagctcgcag gcgatcgcga
    27961 gataacgcct catctgccga gtgatacaac tcaccagcaa tcatcttttc tttttctgtg
    28021 ctcattactg acttccggtt gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga
    28081 agagaacgtt cccattacat ctgtgtgatg aagatcacat agggtatgtc ttcatggcga
    28141 aaaaagtgca aaattcttaa taaacagcca gttatagctc cgaaagtgag ttatttatta
    28201 gcgaataaat ttccatactg aggaagggat cttgtcgtac agtttattca tggtcaattc
    28261 ggcgaggcgg tgatctgcgg ctgagtaaaa taccgccagt tcattatctg ataattcgta
    28321 tttatttttc tcgataacac gctccagcgt gtcaattgtc tggcaacgac gtaaacgcat
    28381 taaataatcg gttttcgtta aaggtttttc ggacatactt ctacccatgg ttgtaataat
    28441 tctaacaaga taaactcgca ggattctctt tcgtcgcatt gacaaaacaa cggaatagtc
    28501 gattacctga cttccgccat ttctgaagat cctgcatatt aataccataa ctactgaaca
    28561 acataaaggt gtcatccaga tattcgtcga tctgctcaat gagcttattg tcttcattat
    28621 acttaatttt gtaattaagt gcgaaggtcg caatatgctc aatcagttca ttcaactgga
    28681 ggttgatcgc cgaggttggg tcgttaaccc agccatgatt gctttcttca aggtttgcaa
    28741 ggcagtcatg atacagggtt tcacagagaa acttaagctg tgcgatatca tgtcttttgg
    28801 gtgagtattc atccataacg cgtccccttc ttagcggttg aactaacgga cacctttcgg
    28861 gatggaaaaa acttactgac ctggacttgc cctctcgttt gttagcttaa ctataagcca
    28921 ctctttgtag gttttcatcg cattattacg aaaaattaca attaggaagg aagcgggaca
    28981 ggactcactt tttcatacta gcaccaaccc gcagcaggta aaagcagttt gctcgaactg
    29041 ttcataacat ttcagaatta tcgccagaag aaattggtga cactaatacc aggattgctc
    29101 tgaatatgac gtaataaccg aggaatgaat aaagaattac cgcaaataat taagaatagc
    29161 ctcta
//
LOCUS       DAZFRG010000032        28834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFRG010000032 DAZFRG010000000
VERSION     DAZFRG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.032"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..1680
                     /gene="yegH"
                     /locus_tag="ACHKUC_001121"
     CDS             97..1680
                     /gene="yegH"
                     /locus_tag="ACHKUC_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6617408.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(1748..3601)
                     /gene="asmA"
                     /locus_tag="ACHKUC_001122"
     CDS             complement(1748..3601)
                     /gene="asmA"
                     /locus_tag="ACHKUC_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO6617409.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3623..4204)
                     /gene="dcd"
                     /locus_tag="ACHKUC_001123"
     CDS             complement(3623..4204)
                     /gene="dcd"
                     /locus_tag="ACHKUC_001123"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO6617410.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4296..4937)
                     /gene="udk"
                     /locus_tag="ACHKUC_001124"
     CDS             complement(4296..4937)
                     /gene="udk"
                     /locus_tag="ACHKUC_001124"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO6617411.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MNSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5255..8572
                     /gene="dgcE"
                     /locus_tag="ACHKUC_001125"
     CDS             5255..8572
                     /gene="dgcE"
                     /locus_tag="ACHKUC_001125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6617412.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNRSYFAIN"
     gene            complement(8682..9530)
                     /gene="alkA"
                     /locus_tag="ACHKUC_001126"
     CDS             complement(8682..9530)
                     /gene="alkA"
                     /locus_tag="ACHKUC_001126"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO6617413.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAVEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANSALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTLAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9664..11016
                     /gene="yegD"
                     /locus_tag="ACHKUC_001127"
     CDS             9664..11016
                     /gene="yegD"
                     /locus_tag="ACHKUC_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6617414.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLIVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPVAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11029..12969)
                     /gene="yegI"
                     /locus_tag="ACHKUC_001128"
     CDS             complement(11029..12969)
                     /gene="yegI"
                     /locus_tag="ACHKUC_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO6617415.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHILFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALVALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKIAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(12966..13727)
                     /gene="pphC"
                     /locus_tag="ACHKUC_001129"
     CDS             complement(12966..13727)
                     /gene="pphC"
                     /locus_tag="ACHKUC_001129"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO6617416.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKLQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13724..14383)
                     /gene="yegL"
                     /locus_tag="ACHKUC_001130"
     CDS             complement(13724..14383)
                     /gene="yegL"
                     /locus_tag="ACHKUC_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6617417.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14601..14657)
                     /locus_tag="ACHKUC_001131"
     CDS             complement(14601..14657)
                     /locus_tag="ACHKUC_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6617418.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(14929..14985)
                     /locus_tag="ACHKUC_001132"
     CDS             complement(14929..14985)
                     /locus_tag="ACHKUC_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6617419.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            15264..16511
                     /gene="mdtA"
                     /locus_tag="ACHKUC_001133"
     CDS             15264..16511
                     /gene="mdtA"
                     /locus_tag="ACHKUC_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO6617420.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSATPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16511..19633
                     /gene="mdtB"
                     /locus_tag="ACHKUC_001134"
     CDS             16511..19633
                     /gene="mdtB"
                     /locus_tag="ACHKUC_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO6617421.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19634..22711
                     /gene="mdtC"
                     /locus_tag="ACHKUC_001135"
     CDS             19634..22711
                     /gene="mdtC"
                     /locus_tag="ACHKUC_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO6617422.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNKGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGAVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22712..24127
                     /gene="mdtD"
                     /locus_tag="ACHKUC_001136"
     CDS             22712..24127
                     /gene="mdtD"
                     /locus_tag="ACHKUC_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6617423.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKTVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSVQ"
     gene            24124..25527
                     /gene="baeS"
                     /locus_tag="ACHKUC_001137"
     CDS             24124..25527
                     /gene="baeS"
                     /locus_tag="ACHKUC_001137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO6617424.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25524..26246
                     /gene="baeR"
                     /locus_tag="ACHKUC_001138"
     CDS             25524..26246
                     /gene="baeR"
                     /locus_tag="ACHKUC_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO6617425.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDCLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26426..26758
                     /gene="yegP"
                     /locus_tag="ACHKUC_001139"
     CDS             26426..26758
                     /gene="yegP"
                     /locus_tag="ACHKUC_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO6617426.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            26905..28266
                     /gene="yegQ"
                     /locus_tag="ACHKUC_001140"
     CDS             26905..28266
                     /gene="yegQ"
                     /locus_tag="ACHKUC_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO6617427.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28595..>28834)
                     /gene="yegR"
                     /locus_tag="ACHKUC_001141"
     CDS             complement(28595..>28834)
                     /gene="yegR"
                     /locus_tag="ACHKUC_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO6617428.1"
                     /translation="IGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVKNDA
                     VHFCVTQGKKMQIVKLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6636 a         7725 c         7628 g         6845 t
ORIGIN      
        1 tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg
       61 acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct
      121 atctgggctg ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg
      181 gtctttattg ccatcctcgc cgaaaaacta ccgcctaagc agcgcgaccg cgcacgggtt
      241 accgggctcc tgctggcgat gttaatgcgc ctgttactgc tggcgtcaat ctcctggctg
      301 gtcaccctga ctcaaccgct gttcagcttc cgctcgttta cctttagcgc ccgcgattta
      361 atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg
      421 ctggaaggga aagacagcaa taaccccacc caacgcaaag gtgcgaagtt ctggggggtg
      481 gtgacacaaa ttgtggtact ggacgccatc ttctcactcg actcggtgat taccgccgtc
      541 gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg
      601 ttgatggcca gcaagccgtt aacccagttc gttaacagtc acccgacgat cgttattctc
      661 tgcttaagct tcctgttgat gattggcttt agcctggtgg cggaaggttt cggcttcgtc
      721 attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga ggcactcaat
      781 cagttggcta tctttaaccg acgacgtttt ctttccgcta accagacact gcgccagcgg
      841 actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc
      901 gaaaccgcgt ccatgctggt ggatcatggt aaccagcaga tctttaatcc gcaggaacgg
      961 cggatgattg agcgggtact taatcttaac cagcgtaccg tcagcagcat tatgacatcg
     1021 cgccacgata ttgagcatat cgatctcaac gcgccggaag acgagatccg ccagttactg
     1081 gagcgaaatc agcatacgcg gcttgttgtt actgatggcg atgacgcaga agatttgctc
     1141 ggtgttgttc acgttatcga cctgttacaa cagtcactgc gcggcgaacc gctcaacctg
     1201 cgggtgttga ttcgtcagcc gctggtgttc ccggaaacct tgccgttgtt acctgccctg
     1261 gagcagttcc gtaatgcccg cacacacttt gcttttgtgg tggatgagtt tggctcggtg
     1321 gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt accgaacgaa
     1381 gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg
     1441 aatggccata tgccgctgga agatctggtg caatatgtgc cgctgccgct ggatgaaaaa
     1501 cgtgaatatc acaccattgc cgggctattg atggaatatt tgcagcgtat tccaaaacct
     1561 ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc
     1621 gtgcagaagg tacagattat tccgctgcat aaggatggcg agatggagta cgaggtgtga
     1681 tggcatgtag gcctgatgcg acgctttacg cgtcttatca ggcctacagg acaggtcacc
     1741 tggtaaccta catcttctcc agcaacttct tcacatcttt gccattgcgg gaatctttat
     1801 tccgctcggc ccagtcgtta agacggcgtt ttgcttcatc ctgtagatgt ttacgcagta
     1861 actgatccac ttgcaggctg taattaagtt gctgccagtt gccataaacc cgcagcggta
     1921 ctggcgtttc tttcaggaaa tcaatcagtt tgctttcccc gttccagcca cccacgaccc
     1981 gaatatcaaa ctgggtgtca caggtttgct ccgccagatt caacatgcct tcccctgtca
     2041 gcgccagcac aggcgattga ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg
     2101 tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc gaagttttca gcggccttca
     2161 catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc atcccttcca
     2221 tgcgcgtgtc ggtcatttcg acatgtgcct gtccttgcca gttatggcgg aatgcgtcgg
     2281 catctatgtc agcaccggag aaatcaccag ccagtgacat ctttccggtc aacgaaatcg
     2341 gatagttaaa cgccttcaga atggtaccaa tctcaacgtt ttccagccgt ggctggaagt
     2401 ttatccgcgg atttattgat gtcgcgtcca acgtgcccgg cagtgaaacc tgtccaccgt
     2461 taagtttgcc ctgcagttga gtaatttcca gcaaacccga cttgttggtc atttgcgtgg
     2521 caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac aaaatatcag
     2581 ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat acgcgaagaa atgaccgggc
     2641 gcggcaacgt tgattggctc tgcccttgct gcgcggcacc gttctcacca ttcgctgttt
     2701 cattaagggg gatgaggttg tcaagattca gttgcgggaa ttgcagtctc agctgccatt
     2761 ccggtttctc tgtcaacgtg acctgtgctt gcccgctcag cgtactgtca ttggcggtca
     2821 aactaatttg gttaaatgaa aggcgtttat gtgactcctg ccactggacc tggaaactcc
     2881 cctgcccttg aataccttgt tttggtaaat cggcaccctg caactgccag ttaatttgtt
     2941 caatagccgc cattaaatca tgcggataat cagaagcatc caccgtaccg ttaagggata
     3001 tcgtgagatc gcgctgatcg cgattaaccc gcccggaaaa ctcaaatgag ccacgatgtt
     3061 ggggatcttg ttccatttgc aggcggatat tgcggattgt cacctgctcg tcatcttcat
     3121 gctggaacac cagaacgctg tccgccacct taagactgga tatatcaaac gaccatccgc
     3181 gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc
     3241 gcaccgcttc cgtctgcggc gtcagttgga tcactgcccc ttttagcatc acctgcttaa
     3301 cgctcagttg atggctcagt agtggtaaaa gcgccacgtc cagacgcatg ttgtctgctc
     3361 gaaccagtgg ctggctcgcg ccctgggcgg tgagagacat tcgcccggag aggatactaa
     3421 gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa ccgctacgtg
     3481 cagcaacttg cttgaccata tagtcgcgga aatcattcgg attcaccagc aacactaacg
     3541 cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga aatcgtctca
     3601 tggtatcctc aatgggcctg aattagtctt tatcgattcg gctggctacc gcgccctgct
     3661 ggttgcgata tttcgcatct tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg
     3721 gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc agcttaccgg
     3781 agttgtagaa ctccagcaca atgcagccag accagcccgg atcgatacgg tgcgcggtga
     3841 cgtgcaccat cagccccaga cgcgccagtg aggaacgccc gtccagccat cccaccagat
     3901 cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagctctcct gggtgaagat
     3961 agaacgcctc gctttcatcg agaacgatct catcgctcat cacgcggtca agcgcggcgc
     4021 tcacttcatc tttgggaccg ctcagatcga taaacgctgc cgtgtgacca cggaaggtac
     4081 gaaatttatt gcccaggcgc acatccaccg tcgcgccgtt aatacgctcc actggcggac
     4141 gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg tcacacagac
     4201 gcatggcatt ttctcctttc gagcatcact cccttaacgc caattacgtc aagggcatac
     4261 caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg acttattttc
     4321 gctttcaata tatcgatcgc gatgcggttt ttcccgccgc gcggcacgat aatgtccgca
     4381 tattgtttag aaggctcaat gaattgcagg aacatcgggc gcacggtttt ttgatattgc
     4441 gccatcactg aattcattga acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc
     4501 aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcgtc acgtaaacgc
     4561 gcatccgtca gcaacaaaat gccttcgaga atgatgacct tcttcggctc aaccgtcacc
     4621 gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat tgccgagccg
     4681 cgtttcaacg cttgtaaatg ctcaagcaac aggctgtgat ccatcgcgct gggatggtcg
     4741 tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta atagcagtct
     4801 tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gtaattcacg ataaagggta
     4861 ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat gacgcactga
     4921 tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg aagggggcga
     4981 cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc tgataccagt cgaatagcgt
     5041 tgccgcgcgc tcagagttaa ttgttgacga agaattcccg gtggcaaatt acgttgatca
     5101 gttttataca aggtaaaaaa tgttatacgc agttgcgcaa attatccgcc tttacgtcac
     5161 tttatgagca attcgcatat aaaatgtaaa acttttgtac tagcataaac acagaaacga
     5221 atactggcga cctggccttg cggataaagc ggtaatgagc aaacaatcac agcatgtatt
     5281 aattgccctg ccccacccgc tgcttcacct ggtcagttta ggtttagtct cgtttatctt
     5341 cacccttttc tcacttgagc tttcgcagtt tggcacccaa ctcgccccac tgtggttccc
     5401 gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc cgggaattgc
     5461 gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca ccagctcgct
     5521 gaacatgacc tggacgacca tcaatattgt tgaagccgtg gtcggggcag tgctgctacg
     5581 taaattgctg ccgtggtata accccttgca aaatctggct gactggctgc gtctggcact
     5641 cggcagcgcc attattccgc ctctgttggg gggtgttctg gttgtcctgc tgacgcccgg
     5701 agacgatcct ctcagggcat ttttgatatg ggtactgtca gaatccatcg gcgctctggc
     5761 actggtgccg ctgggattgt tatttaaacc acactatcta ctgcgccatc gcaacccaag
     5821 gttgcttttt gagtcgctgc tcacgttagc catcacgctg acgttaagct ggctttcgat
     5881 gctgtatctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg
     5941 cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg tgtcgctgat
     6001 gatggccgcg gatccctccc tgcttgctac gccgcgtacg tacctgatga gccatatgcc
     6061 gtggctaccg tttttgctga tcctgctgcc cgccaacatc atgacgatgg tgatgtatgc
     6121 ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga acgccatgga
     6181 atattccgcc atcggcatgg cattagtggg caccgaggga caatggctgc aatccaacaa
     6241 agcgctctgc cagtttctcg gttacagtca ggaagagctg cgcggactca cctttcagca
     6301 actgacctgg ccggaggatc tcaataaaga tctccaacag gttgaaaagc tgataagcgg
     6361 tgaaataaac acctattcaa tggaaaaacg ttactacaac cgcaatggcg atgttgtctg
     6421 ggcgttgctt gccgtctcac tggtgcgcca cacggatggc acgccgctct attttatcgc
     6481 tcagattgaa gacattaacg agctaaaacg caccgaacag gtcaatcaac aactgatgga
     6541 gcgcatcacg ctggccaacg aagcgggcgg gattggcatc tgggagtggg agctgaagcc
     6601 gaatattttt agttgggata agcggatgtt cgagctgtat gaaattcctc cgcatatcaa
     6661 accgaactgg caggtgtggt acgagtgcgt gctgccggaa gatcgccagc acgccgaaaa
     6721 agtgattcgt gattcgttgc aatcacgctc gccctttaaa ctggaatttc gcattgccgt
     6781 gaaagatggc atccgccata tccgcgccct cgccaaccgg gtactgaata aagaaggcga
     6841 agttgaacgc ctgctcggca ttaatatgga tatgacggag gttaaacagc ttaacgaggc
     6901 attgtttcag gaaaaagagc gcctgcacat tacgctggac tccatcggcg aagccgtggt
     6961 ctgtattgat atggcaatga aaattacctt tatgaatcca gtggcggaga agatgagcgg
     7021 ctggacgcag gaagaagcgt taggtgttcc gctcctgacg gtgttgcata ttacttttgg
     7081 cgacaacgga ccattaatgg agaacattta cagtgctgac acctcacgtt ccgcgatcga
     7141 acaagatgtg gtattgcact gccggagcgg cggcagttac gacgtgcatt acagtattac
     7201 gccgttaagt actctggacg gcagcaatat aggctcggta ctggtgattc aggacgttac
     7261 cgaatcacgc aaaatgctgc gccagctgag ctacagcgcc tcccatgatg cactgacgca
     7321 tctcgccaat cgcgccagtt ttgagaagca actgcgtatc ctgctgcaaa cggtaaacag
     7381 tacgcatcag cgacatgccc tggtgtttat cgatcttgat cgctttaaag cggtgaatga
     7441 cagcgccggg catgcggcgg gcgacgcttt actgcgcgaa ctggcgtcgt tgatgctgag
     7501 tatgctgcgc tccagcgacg tgctggcgcg gctcggtggt gatgaatttg gtctgctact
     7561 gccagactgc aatgttgaaa gcgctcgttt tatcgctaca cgcattatca gtgccgtgaa
     7621 tgactatcac tttatatggg aaggacgtgt gcatcgggta ggtgccagtg ccgggattac
     7681 cttgattgat gacaacaatc atcaggcagc tgaagtgatg tcgcaggctg atatcgcctg
     7741 ttatgcctcc aaaaatggtg gccggggccg ggtgacgatt tacgaaccgc agcaagctgc
     7801 cgcacatagc gaacgggcag cgatatcgct tgatgaacag tggcggatga ttaaagagaa
     7861 tcagttgatg atgatcgccc acggcatcgc ttcgccacgg ctcccggaag caagtaattt
     7921 gtggctgatt tcacttaagc tctggagttg cgaaagcgag attattgatg aacaaacatt
     7981 tcgtcgtagc ttcagcgatc cggcacttag ccatgctctt gaccaacggg tatgccacga
     8041 ttttttccag caggccgcaa aagcggttgc cagtaaaggc ttaagcatcg cccttcccct
     8101 ttccgttgct ggtttgagta gcgccacgct ggtgaatgaa ctgcttgagc agctggaaaa
     8161 tagccctcta ccagcacggt tattacatct gattattccg gcagaagcga ttttcgatca
     8221 cgcagaaagc gtgcaaaaac tgcggctggc gggatgtcgg atcgtattca gtcaggtagg
     8281 ccgcgatctg caaatcttca actcgctgaa agcaaatatg gcagattacc tgctacttga
     8341 tggtgagtta tgcgccaacg tgcagggtaa tttgatggat gagatgctga ttacgattat
     8401 tcaggggcac gctcagcgac tcgggatgaa aaccatcgcc gggccagtcg ttttaccctt
     8461 agtgatggat acgctttcgg gcatcggcgt cgatctgatt tatggtgatg tgattgccaa
     8521 tgcccaaccg ctggatttgc tggtgaatag aagttatttc gcgattaact aagggagcgt
     8581 ttgcccattg cctgatgcga cgctaacgcg tcttatcagg cctacaaatc gctcattccc
     8641 caggccggat aaggcgctcg caccgcatcc ggcgaccaac gtcatgcttc gtctggttgc
     8701 cagccttccg tataccagat atgcaacagc gcataagaac gccagggctt ccagcgttcg
     8761 gcataacggc ggatttgtgc cagtgtcatt cccggaaatc gctgtttaat cagataatca
     8821 tccggcagaa aaacatcttt cgcctgccag ccacgcaaag caaaataatt tgccgtccag
     8881 cgtccgatac ccggaaaggt ttgcagcgtt ttcatcgctt gttctacgtc gcccggtatt
     8941 gtcattggta aggtgccctc cagcgccgaa tttgccagat gaatcagcgc ctctgcccgt
     9001 ttcaacggca tacctaacgc ttttaatgcc tgcgggtcgg ctactgccag ccgctgaggc
     9061 gtggggaagc agacataatc cggaaaatca tccagccgtt cgccataaag ctgtgccact
     9121 ctggaggtca attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcacg
     9181 ccctgctcaa cagcgtcaac acaaccaggt aaacgcaatc ctggccgcgc cgcgcctaac
     9241 ttgcccaacg caccgttaac aatctgcggg ttacattgca gatcaaacag gcggctcatt
     9301 ttcgccagac actcagcggc aacaggttct aaacctgcac ttaaatttat gtgcagagta
     9361 tggcgggcta tatccggaat agcagtcacc acgccgcgat attcgcctac cgccagacta
     9421 cgggcataat aactgtccgc gaccgtttcc acgccgctca ccgcacgggc ggcgagaaat
     9481 cccaacatcc acgaccagtc atacggcggc tgccagttca gggtatacat cgcatctcct
     9541 tgttaatccg ctttcagcat aaacgttatt cagacgttac gctttgcttt catattccgg
     9601 ttgtcgcgac ggcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattat
     9661 ttcgtgttta ttggttttga ttacggtaca gcaaactgtt cagtggcggt catgcgcgac
     9721 ggtaaaccgc atttgctaaa aatggaaaac gacagcacgc tgctgccttc aatgctttgc
     9781 gcgccaacgc gtgaagctgt aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac
     9841 gacgatgaaa cgcaggcgct gttgcgtcgg gcgattcgtt ataaccgcga agaagatatc
     9901 gatgttacgg cgaaaagcgt gcagttcggt ctttcctcac tggcacagta cattgatgat
     9961 ccagaagaag tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa
    10021 ccgcagcagg tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccaa
    10081 caggcgcagg cgcagctgcc ggaggcaatt actcaggcgg tgattggtcg cccgatcaac
    10141 ttccaggggc tgggcggtga tgaagcaaac acccaggcgc aagggattct ggaacgcgcg
    10201 gcgaagcgtg ccgggtttaa ggatgtggta ttccagtacg agccggtcgc ggctgggctg
    10261 gattacgaag ccaccttgca ggaagaaaaa cgagtgctga tagtggatat cggcggtggt
    10321 acgactgact gttcattgct gctgatgggg ccgcaatggc gttcgcgtct cgatcgtgaa
    10381 gccagcctgc tgggtcatag tggttgccgt attggtggta acgatctgga tatcgcactg
    10441 gcgtttaaga acctgatgcc gttactgggc atgggtggcg aaaccgaaaa aggcatcgcc
    10501 ctgccgattc tgccgtggtg gaatgcggtt gcgatcaacg acgttcctgc gcaaagtgat
    10561 ttctacagta gtgctaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgtgaaccg
    10621 gaaaaagtgg ccctgttaca gaaagtctgg cgtcagcgtt taagctatcg cctggtgcgt
    10681 agcgcagaag agagcaaaat cgccctttca agtgtagcgg aaacccgcgc ctcactgccg
    10741 tttatcagcg atgaactggc tacgctgatt agccagcaag ggctggaaag cgccctcagc
    10801 cagccactgg cgcggattct ggaacaggtg caactggcgc tggataacgc ccaggaaaaa
    10861 ccggacgtta tctacctgac cggcggtagc gcccgttcgc cgctgattaa aaaagcgctg
    10921 gcagaacagt tgccgggcat tccggttgca ggcggcgatg actttggctc cgtcaccgcc
    10981 gggctggcac gctgggcgga agtggtgttt cgttaattga aaaatggctc agcaacggct
    11041 taaatctgcc tgcgcctgag ccagtttttc tgccgcctga cgtagcggtt ccatcaacgg
    11101 catggtccgt gctggcgcat gaaggcggaa tcgctgcaac tctgtcgcgc cgacagtcag
    11161 cgccgattcc agccgatgtc gtttggcggc tatttttgcc attaccgctt gtctgtctgc
    11221 aggcgttacc gcttcgttcg gcctgaacac aaaacggcgt tcacagctcg ctttccagtc
    11281 gataaccgcc tgggtcagat gatcaccaaa accttttact tgcttaacgc tccgtcgggt
    11341 aacatccgct gctgtttcaa taccaaagga ccgtaacgcc gctttacgcg cagggccaac
    11401 gccgggaata gaggcaacat caataaaaaa tccctccaga aacttctgct tctgccgttc
    11461 ccttgcggtg tcgtgaagtg ctaccagagc gcgtttctct tcttccggta acccgagaat
    11521 ttcgtccttc attttttcga gcatcgcccg tttggcgata aaaccttcca gcccgcccaa
    11581 ctgctggatc tggctgacta aatggtcata gtccattttc gcgcgattaa acgcctcacg
    11641 gcgttgctgg atttctgctt tgtacgcttt gcttgtcagg ctgccaataa tccagatagc
    11701 cgccagcaca ggaatcaaaa taatataacg cggttctgcc tgaaggccgc aaagcaacag
    11761 cgataacgct gacagtgcga tctcaatcag aatgatgtat tcgcgccgta acaggcctga
    11821 aggaagcggc ctgccagtcg gttggaaatg atcgggtaat ggcagttgca atgctggcgg
    11881 tgctactgat gccatcacca tcgcccagac tctcgccagc acaaaatcac cgccggtggt
    11941 aatgacctct tcgccgagat caataaaata gataacgcct tgattatcca gcgcacacca
    12001 cgggcagtca gtcaaatgag cggggtaaac atgcattgcc gaaacggtac atttctttaa
    12061 ctgttggcgt agtaaatcaa gtgccgctac ccacgctttt gccgtcggac gcccggttgc
    12121 cacgccactt tccgtgaatg cctgctgaaa catggcttca acatcgcccg gtaacatcga
    12181 cagcggaatc gatcgtggcg gcggttttaa accacgtcgc tgattatctg aggcgtaggc
    12241 ataacggaaa tgggcaatat ccgtctccag cgcattacct gcatcagaga taagcggtac
    12301 accggaataa ggatgccgcc caccaaacaa gatgtgaaat atcagcaacg caaggccaaa
    12361 attatcgtga ttttctgtgc gttcaaagcc gacaaatgat ggcatggttt gcagctctgg
    12421 cggcgtaaaa tgcgacacgc cgacttcgca taaatgcagt gtaccattgg cattaatctg
    12481 aaaggagtcg ctatcaatca acaccacttt gctgtcgcga cctaccataa agctgttctg
    12541 gtttacgtca cccacgacgt gcccgtgctc atgaacagta gcaaaagatg aagcaatatt
    12601 gcgcgcaaca tagagtagaa aatcccacgc acaatgaggg taactctggc gacgatgtgc
    12661 cgggctatag atcatatgaa tcggttcttt accagaaact tttggcatca taaaaccgat
    12721 aacttttccg cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat aattcaacaa
    12781 ctgcgcgtca gctgtcgcag ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg
    12841 cgtgtgataa atcttggcga cgctatcgac aaactcctcg atatcataaa ccgcaccttc
    12901 gccgcctttg cccagttcac ggcctggcgt aacgcactca ccagtagcag tatataaagt
    12961 gggtttcatg gtaaccacag cgccagtgcc aacgttttat catcatccgt acgctcgttt
    13021 accgccgggc tggacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc
    13081 gttgctgacg ccagtccatt gaaaaacggg gtaaagaaag gcacatgcgg agaattatcc
    13141 agcatattta acgccagtcg ctgaatacca tccgtaaatg ctgcaacttt gtgcgcacgc
    13201 tcagtgctgg tgaaagtttc cagtctggaa acggcatctt catcggtaat gaagtgcgtc
    13261 atattggtat attcacccac catcggcgtg agcggcagtt gcaaaccgtg ccccaaatct
    13321 accaccacgc cgccatcgcc aatctgcata atcaaggtgc cattagctga tgaaatcaat
    13381 cccaaaaacg tgcaggcaaa atcgcgaacc gctaattctt tggcttccgc ctcggcaaaa
    13441 agtcgctgac gaatggtcag cacaatgtcc gttgcgagga catcattaag ccccaattcc
    13501 ccgccctgca atttttgcga catataagcc atcgcttcat tgacggcgag catcgcacct
    13561 tcgccaccct gcgagacgct gcctgcgcca tcagcgacaa ataccgacaa taatggctgc
    13621 tggtcattta gccaggcaac ctgcatctgg caggcatcct ggcaaggcaa atcagcgcta
    13681 atatgcgacg ttccaacggc tgatgcatag accagtcgcc agctcacact gacgtccagc
    13741 cttttggcgc ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg
    13801 aactggataa ccagctaaac agttcacgga attgtaatcc ctgcagagac aaaggctgac
    13861 ggacgctgat ttgggccagc gtcttcatat ccgccccctg aacaccgatg gtaaagaagg
    13921 caaacttctt atcttcttcc ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg
    13981 ttggtgcgcc atcggtgatc aggaaaatcc acggacgata ataggaaata ccattggcac
    14041 gatattcacg tttccgctcc tcaaccatat ccagggcttt ggtaatggca gcgcccattg
    14101 gtgtatcgcc ctgggcaaac aggatgggcg ggaaaaaatt agcggcgctg gtaaagggtt
    14161 gttccacatg caccgggccg aacgtcacaa tgccgagttc cactcttttt aaggccagcg
    14221 aatcggcaag cagctcatcg cgaaaggtaa ccaatccggc gttaagttca ttaatgggtc
    14281 taccgttcat agaaccagaa acatccagca gcaaaatgca ggggcaacgt ggttccgggt
    14341 tactggcaaa atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag
    14401 agaattattg aggtccggta tcataccgat tgcaataatg aaataagagc aaacgaagaa
    14461 actttcagaa ttaggcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag
    14521 agccgtcgat tgacactaag ggcggagtga cataatttca ggagtaaggg tagagcgggg
    14581 tttcccccgc cctggtagtc ttagtaagct ggccagctaa tgactaagag caccacgatg
    14641 atgagtagct tcatcatgac cctttcctta tttaaggccc cttcctcggg aggggctttc
    14701 ccgtttcagc gtcccgctga aatcttcggc ttacttcctt tcgccctgta tgcagtctat
    14761 cgctgacttg cggataaaat agtttcaagt gtgattattg cgtaacgact cgcaaagaat
    14821 atttgctggc aggatcgcag actgcaaagt ctgcggattg acaattggac cgtgaaggca
    14881 tactttcagg agtgagggta gagcggggtt tcccctgccc tggtagtctt agtatgctgg
    14941 gtagctaatc actaagagca ccacgatgat gagtatcttc atcatgaccc tttccttatt
    15001 taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt
    15061 atctcctttc gccatgcaag caatctatcg ctaacgcgta gataaaatag tttcatgtgt
    15121 tattactgga tgcgtgctcg caaaagtgcc cgtcattcag acgattcccg acagtgtttc
    15181 ataattcctc catttttctc ccttattggc tggctacact agtatcattc cgcgaaacgt
    15241 ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg gtaatcgtaa
    15301 tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc aatgactccc
    15361 agagtgcaac cccaggggcg acgaaacaag cgcagcaatc gccagcgggt ggtcgccgtg
    15421 gtatgcgtgc cggcccatta gctccggttc aggcggcgac cgcagtagaa caggctgttc
    15481 cgcgttacct caccgggctt ggcaccatta ccgccgctaa tactgttacg gtgcgcagcc
    15541 gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc aaagcaggcg
    15601 atttactggc agaaattgac ccaagccagt tcaaagttgc attagcacaa gcccagggcc
    15661 aactggcaaa agataaagcc acgctcgcca acgcccgccg tgacctggcg cgttatcaac
    15721 aactggcaaa aaccaatctc gtatctcgtc aggaactgga tgcccaacaa gcgctggtca
    15781 gtgaaaccga aggtaccatt aaggctgatg aagcaagcgt cgccagcgcg caattgcaac
    15841 tcgactggag ccgtatcacc gcaccagttg atggtcgcgt tggtctcaag caggttgatg
    15901 ttggtaacca aatctccagt ggcgacacca ccgggattgt ggtgatcacc caaacacatc
    15961 ctatcgatct ggtctttact ctgccggaaa gcgatatcgc caccgttgta caggcacaaa
    16021 aagccggaaa accgctggtg gtagaagcct gggatcgcac caactcgaag aagttaagtg
    16081 aaggcacgct gttaagcctt gataaccaaa tcgatgccac taccggtacg attaaagtga
    16141 aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt aacgcgcgca
    16201 tgttagtcga caccgaacaa aacgccgtgg tgatccccac cgccgctctg caaatgggca
    16261 acgaaggcca ttttgtctgg gtactgaata gcgaaaacaa agtcagcaaa catctggtga
    16321 caccgggcat tcaggacagt cagaaagtgg tgatccgcgc aggtatttct gcgggcgacc
    16381 gcgtagtgac ggacggcatt gatcgcctga ccgaaggggc gaaagtggaa gtggtggaag
    16441 cccagagcgc cactactccg gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag
    16501 cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc gcctgtttat
    16561 tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg ggattatcgg
    16621 ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccaacca ttcaggtggt
    16681 cacgctttat ccaggtgcga gcccggatgt catgacctct gccgttaccg cgccgctcga
    16741 acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt ccggcggtgc
    16801 gtcagttatc actttacagt tccagctaac attgccgctg gatgtcgccg agcaggaagt
    16861 gcaggccgcg attaacgctg cgaccaacct gctaccgagc gatctgccta acccgccggt
    16921 ttacagcaaa gtgaacccgg cagatccacc gatcatgacg ctcgctgtca cctcaaccgc
    16981 catgccgatg acgcaggtgg aagatatggt ggaaacccgc gtcgcgcaga aaatctcgca
    17041 gatttccggc gtagggctgg tcacactttc cggcgggcag cgtccggcgg tacgcgtaaa
    17101 actcaacgct caggcaattg ccgctctcgg cctgaccagc gaaagtgtgc gcaccgctat
    17161 tactggcgcg aacgttaact cagcgaaagg gagccttgac ggcccttctc gcgcggtcac
    17221 gctttccgcg aacgaccaga tgcaatcggc agaagagtat cgtcagttga ttatcgctta
    17281 tcagaacggc gcaccaattc gtcttggcga tgtcgctact gtagagcaag gagcagaaaa
    17341 cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg ttcagcgcca
    17401 gcccggtgct aacattatct ccaccgccga cagcatccgg cagatgctac cacagctcac
    17461 cgagagtctg ccgaaatcgg tgaaggtgac ggtactttct gaccgtacca ccaatatccg
    17521 cgcatcggtc gacgataccc agtttgagct gatgatggct atcgcgctgg tagtcatgat
    17581 tatctacctg tttttgcgca atattccggc gaccatcatt cccggcgtcg cagtgccgct
    17641 gtcgttaatc ggcaccttcg cggtcatggt gtttctcgat ttttcaatca ataacctgac
    17701 gctgatggca ctaaccatcg ccaccggatt tgtggtcgat gacgccatcg tggtgatcga
    17761 gaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc tcaaaggcgc
    17821 gggtgaaatc ggctttacca ttatctcgct gaccttctcg ctgattgcgg tgttgatccc
    17881 cctgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta ttaccctggc
    17941 agtcgcgatt ttgatctcgg cggtggtgtc gctgaccctg acaccgatga tgtgcgcgcg
    18001 gatgctcagc caggagtcgc tgcgtaaaca gaaccgcttc tcccgtgcct cggagaaaat
    18061 gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga atcatccgtg
    18121 gctgacctta agcgtggcgc tcagcacgct gctgcttagc gtgctgctgt gggtattcat
    18181 tccgaaaggt ttcttcccgg tgcaggacaa cggcattatt cagggcactt tgcaggcacc
    18241 gcaatccagc tcctttgcga atatggccca gcggcaacgc caggtcgcgg acgtgatttt
    18301 gcaggatccg gcagtgcaaa gtttgacctc atttgttggc gttgatggca ctaacccgtc
    18361 gctgaacagt gcgcgtttac agatcaatct caaaccactg gatgaacgag atgaccgggt
    18421 gcaaaaagtc atcgcccgtc tgcaaacggc ggtggataaa gtgccgggcg tcgatctctt
    18481 cctgcaacca acgcaggacc tgactattga tactcaggtc agccgcaccc agtaccagtt
    18541 taccttgcag gccacgtcac tggatgcgct cagtacctgg gtgccacagt tgatggaaaa
    18601 actccagcag ctgccacagc tttctgatgt ctccagcgac tggcaggaca aagggctggt
    18661 ggcgtatgtc aatgtcgaca gggacagcgc cagccgcctg gggatcagca tggcggatgt
    18721 cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt atactcaggc
    18781 taaccagtat cgcgtggtac tggaacacaa caccgaaaat accccaggcc tcgcggcgct
    18841 ggataccatt cgcctgacca gcagcgacgg tggcgtggtg ccgttaagct caattgccaa
    18901 aattgagcag cgttttgcgc cgctttccat caaccatctg gatcagttcc cggtaacgac
    18961 tatctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg cgattatgga
    19021 cacagagaag acgctgaatc tgccggtgga tatcaccacg cagttccagg gcagcaccct
    19081 cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat
    19141 gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca ttctctcgac
    19201 gctacccacc gcaggggtcg gcgcgctgct ggcgttaatg attgccggta gcgaactgga
    19261 tgtgattgcc attatcggca ttattttgct gatcggtatc gtgaagaaga acgccatcat
    19321 gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc gcgaagctat
    19381 ttaccaggct tgtctgttgc gttttcgtcc gatcctgatg accactctgg cggctctgct
    19441 tggtgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc gtccgttagg
    19501 tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttta ccacgccggt
    19561 gatttatctg ctgttcgacc gtctggcatt gtggaccaaa agccgctttg cccgtcatga
    19621 agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg cgacgatttt
    19681 actgtcggtc gccattaccc tgtgcggcat actgggtttc cgtatgctgc cagtcgcccc
    19741 gctgccgcag gtcgattttc cggtgatcat ggtcagcgcc tcgctgcccg gtgcgtcacc
    19801 agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc gcattgccgg
    19861 agtcagcgaa atgacctcca gcagttcgct cggcagcacg cgcattattt tgcagtttga
    19921 ttttgaccgg gatatcaacg gcgcagcgcg cgatgtgcag gcggcgatca acgccgcgca
    19981 aagtctgcta cccagcggga tgcccagccg tccgacctat cgcaaagcga acccatcgga
    20041 tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg aactgtacga
    20101 tttcgcctcg acacagctgg ctccgacgat ttcgcaaatc gacggtgtcg gtgatgtcga
    20161 tgttggcggc agctcgctgc ccgccgtacg cgtcgggctg aatccgcagg cgctgtttaa
    20221 tcagggcgtc tcgctggacg acgtacgcac tgccatcagc aatgccaacg tgcgtaaacc
    20281 gcagggcgca ctggaagatg gcacccaccg ctggcagatc cagaccaatg acgaactgaa
    20341 aactgccgct gaatatcagc cgctgattat tcattacaac aaaggcggcg cggttcgtct
    20401 gggcgatgtg gcgacggtga ccgactctgt acaagatgta cgtaacgccg ggatgaccaa
    20461 cgccaaaccg gccattttgc tgatgatccg caagctgccg gaagccaata ttatccagac
    20521 ggtagacagc atccgcgccc gtctgccgga attacaatcc accatcccgg cagcaattga
    20581 tctgcaaatt gcccaggatc gctcccctac tatccgcgcg tcgctggaag aggttgagca
    20641 gacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttgttcc tgcgttcggg
    20701 tcgcgccacc attattcccg ccgttgcggt gccggtttcg ctgattggta cgtttgcggc
    20761 gatgtacctg tgtggtttca gtctcaataa tctttcgtta atggcgctca ccatcgccac
    20821 tggctttgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc atctggaagc
    20881 ggggatgaaa ccgctacagg ccgcgctgca aggtactcgc gaagtcggct ttacggtgct
    20941 gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg gcggattgcc
    21001 gggtcgcctg ttacgcgagt ttgccgtgac gctttctgtc gccattggta tttcgctgct
    21061 ggtttccctg acgttaacgc cgatgatgtg cggctggatg ctaaaagcca gcaagccgcg
    21121 cgagcaaaag cgactgcgtg gctttggtcg catgttggtt gctctgcaac aaggctacgg
    21181 caagtcacta aaatgggtgc tcaatcatac ccgtctggtg ggcgcggtgc tgcttggcac
    21241 cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc cggagcagga
    21301 caccggcgta ctgatgggcg ggatacaggc tgaccagagt atttcgtttc aggcgatgcg
    21361 cggtaagttg caggacttca tgaaaattat ccgtgacgat ccggcggtgg ataatgtcac
    21421 cggctttacc ggcggttcac gggtgaacag tggaatgatg tttatcaccc ttaaaccacg
    21481 cggcgagcgg agcgaaacgg cccaacagat tatcgatcgc ttgcgtaaaa aactggcgaa
    21541 agaaccggga gcgaatctgt tcctgatggc ggtacaggat attcgcgttg gcggacgtca
    21601 ggctaacgcc agctaccagt acacattatt gtccgacgat ctggcggcac tgcgcgaatg
    21661 ggagccaaaa atccgcaaaa aactggcgac attgccagag ctggctgatg ttaactccga
    21721 tcaggaagat aatggcgcgg agatgaacct tatttacgat cgcgacacta tggcgcgatt
    21781 gggaattgac gtgcaagccg ccaacagcct gttaaataac gccttcggtc agcggcaaat
    21841 ctcgaccatt taccagccga tgaaccagta caaagtggtg atggaagttg atccgcgcta
    21901 tacccaggac atcagtgcac tggaaaaaat gttcgttatc aataacgaag gaaaagcgat
    21961 cccgctgtcg tatttcgcta aatggcaacc ggcgaacgct ccactgtcgg tgaatcatca
    22021 gggattatcg gcggcctcga ccatttcgtt caacctgccg accgggaaat cgctctcgga
    22081 cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga cggtgcgcgg
    22141 cagttttgcc ggaacagcgc aagtgttcca ggagacgatg aactcacagg tgatcctgat
    22201 tatcgccgcc atcgccacgg tatatatcgt actgggtatc ctttacgaga gttatgtgca
    22261 tccactgacg attctctcca ccctaccctc ggcgggcgtc ggagcgctgt tggcgctgga
    22321 gctgttcaat gccccgttca gcctaattgc gctgataggg atcatgttat taatcggcat
    22381 cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac ggcacggtaa
    22441 cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc cgattatgat
    22501 gactaccctg gcggcgctgt ttggtgcgct gccgctggta ttgtcgggcg gtgacggctc
    22561 ggagttgcgg caaccattgg ggatcaccat tgtcggcggt ctggtcatga gccagcttct
    22621 gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc tgcgtttttc
    22681 gcgtaaacct aaacaagcgg taaccgagta aatgacagat cttcccgaca gcacccgctg
    22741 gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca ccatcgtaaa
    22801 caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata tgcacatggt
    22861 cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct ggctggcgga
    22921 caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc tcggttcact
    22981 gttttgcgca ctttccggca cgctgaacga actgttgctg gcacgcgcgt tacagggcgt
    23041 tggcggtgcg atgatggtgc cggtcggtag gttgacggtg atgaaaaccg taccgcgcga
    23101 gcaatatatg gcggcgatga cctttgtcac gctgcccggt caggtcgggc cgctgcttgg
    23161 cccggcgctc ggcggtctgc tggtggagta cgcatcgtgg cactggatct ttttgatcaa
    23221 cattccggtg gggattattg gggcgatcgc cacactgatg ttaatgccga actacaccat
    23281 gcagacgcgg cgctttgatc tctccggctt tctgttgctg gcggttggca tggcggtatt
    23341 aaccctggcg ctggacggca gtaaaggtac aggtttctcg ccgctggcga ttgctggcct
    23401 ggtcgcagtt ggcgtggtag cactggtgct ttatctgctg cacgcccaaa ataacaaccg
    23461 cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc tggcggggag
    23521 ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt tcctgcaaat
    23581 tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg tactcggcag
    23641 catgggaatg aagagaattg tggtacaggt agtgaatcgc tttggttatc gtcgggtact
    23701 ggtggcgacc acgctgggcc tgtcgctggt caccctgttg tttatgacta ccgctctgct
    23761 gggctggtac tacgttttgc cgttcgtcct gtttttacaa ggaatggtca actcgacacg
    23821 tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaacctgg cgagcagcgg
    23881 caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt attggcgtca ccatcgccgg
    23941 gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca ccacacaaac
    24001 cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg cgtttgtctt
    24061 tgccagagtg ccgagcgata cgcatcaaaa tgtcgctatt tcacggcgga aaaggagcgt
    24121 gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat tttcgctacc
    24181 tgcattgtct tattgatcac gatgcactgg gcggtacgta tcagttttga gcgcggcttt
    24241 atcgattaca tcaagcatgg taatgaacaa cggctgcaaa tgctcggcga tgcgcttggt
    24301 gagcagtacg cccagcacgg gaactggcgc ttcctgcgta ataacgatcg ctttgtattt
    24361 cagatcctac gttcactgga gcatgataac aacgaagata agcccggccc cggtatgccg
    24421 ccacacggct ggcgcacaca attttgggtg gttgatcaaa acaacaaagt gctggttggc
    24481 ccgcgagcac cggttccacc cgacggcaca cggcggccca ttatggtcaa tggtgcggaa
    24541 gttggcgcgg tgatcgcctc ccctgttgaa cgactgaccc gtaatactga tatcaatttt
    24601 gacagacaac agcggcaaac cagttggctg attgtggctt tatctacctt attagcggcg
    24661 ctggcgacat tcccactggc gcgcggtttg ctggctccgg taaaacgact ggtggatggc
    24721 acgcacaaac tggcagcggg tgatttcact actcgcgtag cccccaccag cgaagatgag
    24781 ttgggcagac tggcagagga tttcaatcaa ctcgccagca cactggagaa aaaccagcag
    24841 atgcgccgcg attttatggc cgatatttct catgaactgc gcacgccttt agcagtactg
    24901 cgcggcgaac tggaagccat tcaggatggt gtgcgtaaat tcacgccgga gacgctggct
    24961 tctttacagg cagaagtcgg tacactgaca aaactggttg acgatctcca tcaattgtct
    25021 atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat cccactgctg
    25081 gaagtggcgg gaggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa attgcaattt
    25141 tccctgccag acagtattac cgtatttggc gatcgcgatc gtttaatgca gttgttcaat
    25201 aacttactgg aaaatagcct gcgctacact gacagcggcg gcagcctgaa aatctctgcc
    25261 gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccggg tgtcagtgac
    25321 gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg caaccgcgcc
    25381 agcggtggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc acataacggc
    25441 cgcattattg ccgcccattc gccttttggc gggcttagca ttacagtaga gttaccgctg
    25501 gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac acaccgcgta
    25561 ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat ctgcgtgctg
    25621 cgagctatgc gccaacgttg attagccacg gcgatcaggt actgccatat gtgcgccaga
    25681 caccaccgga tctgatactg ttggatctga tgctccctgg caccgatggc ctgacgctgt
    25741 gtcgggagat tcgtcgtttt tctgacattc cgatcgtgat ggtgacagca aaaatcgaag
    25801 agatcgactg cctgctcgga ctggagattg gcgcagatga ttatatctgc aagccgtaca
    25861 gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa ccgcagcgcg
    25921 agttgcagca acaggatgct gaaagcccgt tgattatcga cgaaggtcgt tttcaggctt
    25981 cctggcgcgg taaaatgctt gacctgacgc ctgcagaatt tcgtctgctg aaaacgctct
    26041 ctcatgaacc aggaaaagtg ttctcccgcg agcagttgct gaaccatctt tatgacgact
    26101 accgcgtggt aaccgaccgt accatcgaca gccacattaa aaacctgcgc cgcaagctgg
    26161 aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgtcggt taccgctggg
    26221 aagccgacgc ctgccgcatc gtttaacatt tttacctgcc ggagatggcc ttcagaccag
    26281 atccggcaac attatcccac gcatggtcag caaactgaca ttatctctcc gccctggcag
    26341 gcaccagaaa atcgctcata ctttaattgg caaacagtac ctttagatgc tgttttcgat
    26401 acgcaatttc aatcaaggag tcattatggc tggttggttt gaactcagta agagcagtga
    26461 taatcagttc cggtttgtgc taaaagcggg caatggtgag actatcctca ccagcgagct
    26521 gtatacctca aaagcctctg cggaaaaggg catcgcgtcg gtgcgtagca acagcccgca
    26581 agaagaacgc tatgagaaaa aaacggcaag caatggcaaa ttctatttca atctgaaagc
    26641 cgccaatcat caaattatcg gctccagcca gatgtatgcc accgcgcaat ctcgtgaaac
    26701 cggaatagcc tccgttaaag taaatggcac aagccagacg gtgaaagaca atacgtaatt
    26761 acgatgccgg gcgtaggttg ttggcgtccg gcgctttacc agttaccccc acagcgctac
    26821 aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga agcggctctg
    26881 acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc ggcgggaacg
    26941 ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc gggccagccg
    27001 cgttactccc tgcgtgtacg caacaacgaa ttcaaccacg aaaatcttca gctcggcatc
    27061 aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac
    27121 gccaagctga aaacctttat ccgtgacctg aaaccggtgg tggaaatggg gccggatgcg
    27181 ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agcacttccc ggaaatgcca
    27241 atccacctct cggtacaggc taacgccgta aactgggcga cggtgaaatt ctggcagcaa
    27301 atgggtctga cccgcgtgat cctctctcgc gaactgtcac tggaagagat tgaagagatc
    27361 cgcaatcagg tgccggatat ggagatcgaa atcttcgttc acggcgcgct gtgcatggcc
    27421 tactccggtc gctgcctgct ctctggctat atcaacaagc gcgacccgaa ccagggcacc
    27481 tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga tgacgttggc
    27541 aacatcgtgc acaagtacga gccgattccg gtgcaaaatg ttgagccgac gctgggcatc
    27601 ggcgcgccaa ccgacaaagt gtttatgatc gaagaagctc agcgtccggg cgagtatatg
    27661 actgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct gcgcgctatc
    27721 gcccacgttg aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga aggtcgtacc
    27781 aaatctttct actattgcgc acgcaccgcg caggtttacc gtaaagctat cgatgacgca
    27841 gctgcgggaa aaccattcga taccagcctg ctggaaactc tggaaggtct ggcgcatcgt
    27901 ggctataccg aaggtttcct gcgtcgtcat actcacgacg attatcagaa ctacgaatac
    27961 ggttattcag tttctgaccg ccagcagttt gttggtgagt ttaccggtga gcgcaagggc
    28021 gacctcgcgg cggtagcggt gaaaaataaa ttctccgttg gcgacagcct tgagctgatg
    28081 acgccgcaag gcaacattaa ctttaccctt gagcatatgg aaaacgccaa aggtgaagca
    28141 atgccggtcg caccaggcga tggttatact gtgtggatcc cggtcccgca ggatcttgag
    28201 ctaaattacg cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa cccccacggt
    28261 aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg atcacataca
    28321 gctgcattta ttaaggttat catccgtttc gctgaaaaac ataacccata aaatgctagc
    28381 tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt attttttatg
    28441 cataagccgt atcctggtaa ccgtcttcca ttgaccacat cgatagaatc tcccttcata
    28501 gcacgatgcc tttcacttat tggcatcgtg ctcgcacagg ttccggttac gcacagccag
    28561 aacgcgcatg tttgacgctt accaaaaaat attctcactc tccacatttg aatgtcagac
    28621 gagcgacgcc atgtaatcct gcaccttctg tcttcaattt aactatctgc atttttttgc
    28681 cctgagtaac acagaaatga actgcatcat tttttactat attttctgca ccagatattc
    28741 tacccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa
    28801 tattactttt atatgcaaga ccaaacttac cgat
//
LOCUS       DAZFRG010000033        28734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFRG010000033 DAZFRG010000000
VERSION     DAZFRG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.033"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..969
                     /locus_tag="ACHKUC_001142"
     CDS             <1..969
                     /locus_tag="ACHKUC_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6617429.1"
                     /translation="TRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYYAGVRQ
                     GVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVRR
                     ASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIA
                     VINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISTPDLNA
                     IMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMG
                     NAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            992..2503
                     /locus_tag="ACHKUC_001143"
     CDS             992..2503
                     /locus_tag="ACHKUC_001143"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6617430.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2528..3526
                     /locus_tag="ACHKUC_001144"
     CDS             2528..3526
                     /locus_tag="ACHKUC_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6617431.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3559..4653
                     /locus_tag="ACHKUC_001145"
     CDS             3559..4653
                     /locus_tag="ACHKUC_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6617432.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLVEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4665..5537
                     /locus_tag="ACHKUC_001146"
     CDS             4665..5537
                     /locus_tag="ACHKUC_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6617433.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(5697..6119)
                     /locus_tag="ACHKUC_001147"
     CDS             complement(5697..6119)
                     /locus_tag="ACHKUC_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6617434.1"
                     /translation="MNSLRYFDFGTSRPVLLLIARIAVVLIFIIFGFPKMMGFGGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            6488..7627
                     /locus_tag="ACHKUC_001148"
     CDS             6488..7627
                     /locus_tag="ACHKUC_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO6617435.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(7619..8899)
                     /gene="csiE"
                     /locus_tag="ACHKUC_001149"
     CDS             complement(7619..8899)
                     /gene="csiE"
                     /locus_tag="ACHKUC_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO6617436.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTANTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(9090..9944)
                     /locus_tag="ACHKUC_001150"
     CDS             complement(9090..9944)
                     /locus_tag="ACHKUC_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6617437.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTKSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(10089..10892)
                     /gene="suhB"
                     /locus_tag="ACHKUC_001151"
     CDS             complement(10089..10892)
                     /gene="suhB"
                     /locus_tag="ACHKUC_001151"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO6617438.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            11011..11751
                     /gene="trmJ"
                     /locus_tag="ACHKUC_001152"
     CDS             11011..11751
                     /gene="trmJ"
                     /locus_tag="ACHKUC_001152"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO6617439.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            12022..12510
                     /gene="iscR"
                     /locus_tag="ACHKUC_001153"
     CDS             12022..12510
                     /gene="iscR"
                     /locus_tag="ACHKUC_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO6617440.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            12622..13836
                     /gene="iscS"
                     /locus_tag="ACHKUC_001154"
     CDS             12622..13836
                     /gene="iscS"
                     /locus_tag="ACHKUC_001154"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO6617441.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            13864..14250
                     /gene="iscU"
                     /locus_tag="ACHKUC_001155"
     CDS             13864..14250
                     /gene="iscU"
                     /locus_tag="ACHKUC_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO6617442.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            14267..14590
                     /gene="iscA"
                     /locus_tag="ACHKUC_001156"
     CDS             14267..14590
                     /gene="iscA"
                     /locus_tag="ACHKUC_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO6617443.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            14686..15201
                     /gene="hscB"
                     /locus_tag="ACHKUC_001157"
     CDS             14686..15201
                     /gene="hscB"
                     /locus_tag="ACHKUC_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO6617444.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            15218..17068
                     /gene="hscA"
                     /locus_tag="ACHKUC_001158"
     CDS             15218..17068
                     /gene="hscA"
                     /locus_tag="ACHKUC_001158"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO6617445.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQAIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            17070..17405
                     /gene="fdx"
                     /locus_tag="ACHKUC_001159"
     CDS             17070..17405
                     /gene="fdx"
                     /locus_tag="ACHKUC_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO6617446.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            17417..17617
                     /gene="iscX"
                     /locus_tag="ACHKUC_001160"
     CDS             17417..17617
                     /gene="iscX"
                     /locus_tag="ACHKUC_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO6617447.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            17676..18959
                     /gene="pepB"
                     /locus_tag="ACHKUC_001161"
     CDS             17676..18959
                     /gene="pepB"
                     /locus_tag="ACHKUC_001161"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO6617448.1"
                     /translation="MTEAMKITLSPQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            19102..19878
                     /gene="sseB"
                     /locus_tag="ACHKUC_001162"
     CDS             19102..19878
                     /gene="sseB"
                     /locus_tag="ACHKUC_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO6617449.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEGEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19910..20035)
                     /gene="timP"
                     /locus_tag="ACHKUC_001163"
     CDS             complement(19910..20035)
                     /gene="timP"
                     /locus_tag="ACHKUC_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO6617450.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGNNLLVVNNLQNSK"
     gene            complement(20371..21216)
                     /gene="sseA"
                     /locus_tag="ACHKUC_001164"
     CDS             complement(20371..21216)
                     /gene="sseA"
                     /locus_tag="ACHKUC_001164"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO6617451.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVTNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            21423..26384
                     /gene="yfhM"
                     /locus_tag="ACHKUC_001165"
     CDS             21423..26384
                     /gene="yfhM"
                     /locus_tag="ACHKUC_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO6617452.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSHNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     SSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPVKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            26385..28697
                     /gene="pbpC"
                     /locus_tag="ACHKUC_001166"
     CDS             26385..28697
                     /gene="pbpC"
                     /locus_tag="ACHKUC_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO6617453.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
BASE COUNT         6721 a         7059 c         8198 g         6756 t
ORIGIN      
        1 acaagaaccc gtaatttatt attaatggct acactattgg gtagcgcact atttgccagg
       61 gcttctgata aagaaatgac tattggcgca atataccttg atacccaggg atattacgcc
      121 ggagtgcgtc agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt
      181 gaaactaacg ctcagggcga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg
      241 cgtaatgtcg atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt
      301 cgtcgcgcca gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag
      361 ggtgtcgata aatatgtctc ggcatatctg gttggtgacc cgctggaatt tggtaaaaaa
      421 ctgggtaacg cagccgccga ttattttatt gccaacaaaa ttgaccagcc gaaaattgcc
      481 gtcatcaatt gcgaagcctt tgaagtttgt gtgcaacgac gtaaagggtt tgaagaagta
      541 ttaaaagccc gcgttcccgg cgcgcaaatt gtcgctaatc aggaagggac tgttttagat
      601 aaagcgattt ctgtcggtga aaaactgatt atctccacgc cggatctcaa cgccattatg
      661 ggggaatcgg gcggagcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc
      721 ggaaaaattg ctgttttcgg ttctgatatg accactgaaa ttgctcagga gctggaaaac
      781 aaccaggtgc tgaaagcggt agtggatatt tccggtaaga aaatgggcaa tgctgttttt
      841 acgcaaacat tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca
      901 atcgatctct ataccaaaac ggaagacggt aaacagtggc tggcaacgca cgttgatggt
      961 ctgccctaat cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt
     1021 agcaaaagtg gtggcaggaa ataagcgtta tcccggcgtc gttgcgctgg ataacgttaa
     1081 cttcacgctc aataaaggcg aagttcgtgc gctgttaggc aaaaacggtg cgggaaaatc
     1141 gaccctcatt cgaatgctta ccggtagcga acgtccggat agcggtgata tctggattgg
     1201 cgagacgcga ctggaaggtg acgaagctac gctgactcgc cgtgccgctg aactgggggt
     1261 acgcgcggtt tatcaggagt taagtctggt ggaagggctg acagtggcgg aaaacctctg
     1321 cctcggtcag tggccccgcc gcaacggcat gattgattac ctgcaaatgg cgcaggacgc
     1381 ccaacgttgc ttgcaggcgt tgggcgttga cgttagtcct gaacaacttg tttcaacact
     1441 aagcccggcg caaaagcagc tggtggaaat tgcgcgggtg atgaagggcg agccgcgcgt
     1501 ggtcattctt gatgaaccca ccagctcgct tgccagtgcg gaggttgaac tggtgatcag
     1561 cgcggtgaaa aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga
     1621 agaaattcgc cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga
     1681 tgtgatgctc gaaaacacct ccacgcatca tattgtgtcg ctaatgctcg ggcgcgatca
     1741 cgttgatatt gcgccggttg cccctcagga aattatggat caagccgtgc tggaagtccg
     1801 tgcgttacgc cataagccca agctggagga tatcagtttt acactacgtc gtggcgaagt
     1861 gctcggcatt gctggtctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg
     1921 gctggagacg tatgaacagg gcgaaattgt tatcaacggc gagaaaatca cgcgccccga
     1981 ttacggcgac atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg
     2041 gatcattccc tggttgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag
     2101 cgccaacggt gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg
     2161 gatgacggtc aaggccgcca gtagcgaaac gcccatcggc acgctttctg gtggcaatca
     2221 gcaaaaagtg gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga
     2281 gccaacgcgt ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgcgagct
     2341 ggctgccgaa ggaaaaagcg tggtgtttat ctccagtgaa gtggaggagt tgccgctggt
     2401 gtgtgaccgc atcctgttat tacagcacgg tacgttctcg caggagtttc actcgccggt
     2461 caatgtggat gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa
     2521 cctgaagatg tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca
     2581 atttgtcagt cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct
     2641 ggtcttctcc ctgaatgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg
     2701 cgatgccgcc accattggga tcgccgcctg ggcgatgacg ctgattatta tctccggtga
     2761 aattgatgtc agcgttgggc cgatggtggc ttttgtttca gtgtgcctgg cgtttttgct
     2821 gcaatttgac gttccgctgg cgattgcttg tctgctggtg ttgctgttag gtgcgctgat
     2881 gggaacgctc gccggggtgc tgcgcggcgt gtttaacgtg ccaagttttg ttgccacgct
     2941 gggtttatgg agcgccctgc gcggaatggg gctgtttatg acgaacgcct tgccagtgcc
     3001 aattaacgaa aacgaagtgc tggactggct gggcggacaa tttctcgggg tgccggtatc
     3061 cgcgctgatc atgatggtgt tatttgcgct gtttgtgttc attagccgca aaaccgcctt
     3121 cgggcgctcg gtttttgctg ttggcggtaa tgccactgca gcgcagttgt gcggtattaa
     3181 cgttcgtcgg gtacgcattc ttatttttac cctttcggga ttattagcgg cggtgaccgg
     3241 cattttgttg gcggcgcgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
     3301 tgacgtcatc gccgcggtcg ttgtcggcgg tacggcgctt tccggcggtc gcggctcgtt
     3361 gttcggtacg ctgcttggcg tactggtgat tacgctaatc ggtaacggtc tggtgctgct
     3421 cgggattaac tcctttttcc agcaggtggt gcgcggcgtc atcatcgtgg tggcggtgct
     3481 ggcgaatatc ttgctgaccc agcgtagcag taaagcgaaa cgctaaacct taacgcccct
     3541 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
     3601 atgctggcag cttatttacc aggaaattcg accgtcgatc tgcgggaagt tgcggtgccg
     3661 acgccgggga ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat
     3721 gtccactata tctaccacca gcaccgcgct acggcggcgg cacccgataa accgttatac
     3781 cagggattta tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctgc
     3841 cgccatttta aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc
     3901 ccgaactgcc gtcgcggttt tcctatttct tgtactggcg aaggaaaagc ggcttacggc
     3961 tggcagcgtg acggcggcca tgctgaatac ctgctggtgg aagaaaaaga tctgatcctc
     4021 ctgccggatg cactgagtta cgaagatggt gcgtttatca gttgcggcgt tggtacagcg
     4081 tatgaaggga ttttgcgcgg cgaagtttcc ggcagtgata acgtactggt ggtcggtctg
     4141 gggccggtcg gcatgatggc gatgatgctg gcgaaaggtc gtggtgcaaa aaggatcatc
     4201 ggcgttgata tgctgccgga acgtctggcg atggcgaaac agctaggggt gatggatcac
     4261 ggctatttag cgaccaccga aggtctgccg cagatcatcg ctgaactcac tcatggcggt
     4321 gcggatgttg cgcttgattg ttccggtaat gctgcggggc gcttgctggc attgcaatct
     4381 accgccgact gggggcgagt ggtttacatt ggcgaaaccg gaaaagtgga atttgaggtc
     4441 agcgctgacc tgatgcatca ccaacgcaga attattggct cctgggtgac cagcttgttc
     4501 catatggaaa aatgcgccca cgatttgact gactggaaac tgtggccgcg taacgctatt
     4561 acccatcgct tctcactgga gcaggcggga gatgcttacg cgctaatggc gagcggcaaa
     4621 tgcgggaaag ttgtgattaa tttcccggat taaggagtct gcggatgacc atctatacct
     4681 tatctcacgg gccgctaaag ctggacgttt ctgatcaggg cggcgtaatc gaaggcttct
     4741 ggcgcgatac gacgccgctg ctacgtcctg gtaaaaaaag cggtgtggca accgatgcct
     4801 cttgttttcc gctggtacca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg
     4861 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg
     4921 gctggttggg cgagtggcaa tgtgtctcgc gcagcgatga tagcttgtgt ctggtgtatg
     4981 aacatctcag cggtgtttat cactatcggg taagccaggc atttcattta acggcggata
     5041 cgctgacggt gacgctcgct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     5101 gctggcatcc ttattttccg ttgtcgccgc aaacgcggat tcaggcacag gcgagcggtt
     5161 actggctgga gcgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggagc
     5221 tggattttaa ccagctcgcg ccgttgccgc gccagtgggt aaacaatggt tttgccggat
     5281 ggaatggtaa ggcccgcatc gagcagccgc aggagggtta tgccatcatc atggaaacga
     5341 cgccacctgc accgtgttat ttcatctttg tttcagaccc tgcgtttgat aaaggatatg
     5401 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc ggatgaccat catcgtccgg
     5461 aaggtggtga cctcattgcg cttgcaccag gggaatcaac aatttcagag atgtcgttgc
     5521 gggttgcgtt gctgtaattt ataaaatgcc ggatgcacag gcctacgggg taatttcgat
     5581 tttgtagcac tcggtgcacg atgcctgatg cgacgcttgc tgcgtcttat caggcctaca
     5641 atcggtgttg gagcggtagg ccggataagg cgtttacgcc gcatccggca tctttcctaa
     5701 cggcgatcga gagaaatcgc ccccggcccg gtaattgcca acagcaggaa tgcgccagcg
     5761 atactgacat tcttccagaa attaatcata tttggcccaa ccgcatcgcc ggtcatatcc
     5821 cagtaatggt gaccaattac cgctgtaccc agcgtgtaga aaataaacag caccgccagc
     5881 ggacgggtga aaaagccaag cacgattaat atcgcggcgg gcacttccat aactaccgca
     5941 ataatcgctg ccagcatcgg cattggtgcg cccagcgagg ccatatattg gaccgtaccg
     6001 ccaaagccca tcattttggg aaaaccaaaa ataatgaaaa ttaagaccac ggcgatacgg
     6061 gcaattaata ataaaacggg gcgggaagtg ccaaaatcaa aataacgtag tgagttcata
     6121 accaatccct ttgcaagatg atgtgattta aacgtaatac acactctggt acctcgccac
     6181 gcccaggcgt tgagatattc gtttctggta taacggcata acgcagatgg ctgacaatgt
     6241 gcttattgtt tcctgccgga tgcgacatgc acgccttatc cgtctcacat gagtttgtca
     6301 gttcaataaa ttgcgatttt cacctgtttc atcgttcttt tttacatgtt gtgttgtggg
     6361 ccgctctttt ttgtcatttc ctgatatgca ggataaccgt caggaagcag gctcgaatat
     6421 tcttattttt caacttgcac caacattcga ttaacctcag actaacgcct cctgacggga
     6481 gggactcatg gttttgcaat ccacgcgctg gttggcgctc ggctatttca catacttttt
     6541 tagttacggc atttttctac ctttctggag cgtctggctt aaagggattg gtttaacgcc
     6601 agaaaccatc ggcctgttat tgggggcggg tctggttgcc cgtttcctcg ggagtttgct
     6661 catcgcgccc cgcgtcagcg atccttcccg cctgatttcc gccttgcgcg tgctggcact
     6721 gctgacactt ctctttgctg tcgccttctg ggcgggggcg cacgtagcgt ggctgatgct
     6781 ggtgatgatt ggctttaacc tctttttctc accgctggta ccgttgaccg atgcactggc
     6841 gaatacgtgg caaaagcagt tcccgcttga ttacggcaaa gtgagactgt ggggctcggt
     6901 ggcgtttgtc attggctcgg cgctgacggg caaactggtc agtatgtttg attatcgggt
     6961 gatcctcgcg ctgttgacgt tgggcgtggc atccatgctg ctcggctttc tcatccgtcc
     7021 gacgattcag ccacaagggg caagccgcca gcaggagagc accggctggt ctgcgtggtt
     7081 ggcgttggtt cgccagaact ggcgctttct ggcctgcgtt tgtttattgc agggggcaca
     7141 tgcggcctat tacggtttta gcgccattta ctggcaggca gctggctact cggcctcggc
     7201 ggtggggtat ttgtggtcgc tgggcgtggt ggcggaagtc attatctttg cgctgagtaa
     7261 taaacttttc cgccgttgta gtgcacgcga tatgctgttg atctcggcga tttgcggcgt
     7321 agtgcgctgg ggcattatgg gagcaactac ggcgttgccg tggttgatag tggtgcaaat
     7381 tctgcattgc ggtaccttca cggtctgcca cctggctgcc atgcgctata ttgctgctcg
     7441 ccagggtagc gaagtcatcc gtttacaggc ggtttactct gccgtcgcga tgggcggcag
     7501 tatcgccatc atgaccgttt tcgccggttt cctgtatcaa tatctcggcc acggcgtgtt
     7561 ctgggtgatg gcgctggtgg cgcttccggc aatgtttttg cgcccgaaag ttgttccctc
     7621 atgctgattc gagcattttg cggatctgct cctgttgatg tgttgtcaac gtcaggtccg
     7681 tatagatcag cggtggtgaa aagagcggta acggcatggt ataaggcgca ataatcagtg
     7741 ccgcgccgcg cggagcgcct gtctgcaaaa atgcctttac cgacatatgt ttaatgttga
     7801 gcggcagtaa cgttagttcg cgtagctgct gctcaatctg cgcttctcgc tcgctatcat
     7861 tcccggtcaa tagaataatc tgtttctcat gcaggtcgtt ttcctgcatt agccaggcac
     7921 caaaaatcac cgcgaccaga ccgctttcct catcagaaag atggacgccg tattcacttt
     7981 caaatccagc cagcgccgcg cgggtggtgc gcaccaggcg tggatacagg cgggcgaact
     8041 cttccggcag ggtgttatca ataccgatgg caaacaaact gcgatttaac gcctgggcga
     8101 ggtgggtata aagctgatcg cataacccct gttcatcata aaaacgaaca tttcccagct
     8161 cacgaaagtg gtttaccaga cgtttgatag acttacgcag ttgtctgtcc cgctgatgcg
     8221 catcccgcag tggatcggga acccgcaaca tggaaaaaag taatgccata aacagtggct
     8281 catcaggcgg tacaggctgg agcgcccgac gctgccagtg gcgtccaatt tcctgcgcta
     8341 cctgaaattc aaggcaggat tccgcccagc gacgttggag cggattaaac tgaggcgtaa
     8401 ttccagcgtg gtgttgcagc aaacaatatt gcagatacag acacaggaaa tgaatatcgc
     8461 gcgtttcaaa acgtttttgc agccgtcgag agcagagatt cactaacgct tgtagattgg
     8521 tatcgtcgta aaagttacgc gcaattccgc gccgcttcag ttcactcttc agggcggggg
     8581 tgaatcggct ggtaataaat gacggacaca gacggaaacc acgtcgtagc cagtgaaata
     8641 aacacaaacg ttggttaagc actgtacctt caacccggta gctgccgtca taaccagtag
     8701 tcagtgtgag ttgatgatag cgcaggatct cccgtcctgt ttcgctgata tcaagactgg
     8761 caatatcatc atccacacca ttaagctcgc tgaaagttgc cgtgttggcg gtcaaccccg
     8821 gctgaaagag cgtcagcaag atctggcagc ggcgctgggg agccgaaagg acagatggtg
     8881 gagcaagcgt aggcatcatc tgtagatctc ccataagcat aattgataag aatagctaaa
     8941 gtttgcttag ggaaatggcg aagggaatgc taatcatcag aaatgttggc aagcatcaca
     9001 gaatttttct cattttaaac tgttctgacc agtggattag catgtcttaa ggtggttttg
     9061 tgaatgatta tcgtgctagc gatcatgggt tagttctgcg gattcaacgc gctaagaata
     9121 aaatccacca tctgttcgcg ataaacatcg ccgtgacgat cggaaaaagc atcaatgtgt
     9181 tcgccatccg ggattaggat caaccgtttt ggttcttttg ccaggctata caacttttca
     9241 ctgtgctgcc acgggataac gtgatcagct ttaccgtgaa tgagtaaaag cgggatcggg
     9301 ctgacgctgg cgatgtaatt ttcgccgctg taactctcat caagtaagta gccactgccg
     9361 gggatcatct ggttggcaat ggtcgcataa gaggcaaatg tggagtcgag gatcaccgca
     9421 cgtatgcctt cacgatcatc ctgaccaata acatccagaa tattcgcccc gccaatactc
     9481 tgcccgaaca gcaccagccg ttgtgggttt acatcgctgc gatggcgcac cacattgatg
     9541 gcacttttcg tatcgtccag caatccggcc tgggacggcg tgccttttga tttaccaaac
     9601 ccgcgataat caaacataaa aacgttgaaa ttacgctcgg gtaaccaact gaccagcggc
     9661 cagtgggcgg acatatttcc ggcattgccg tgagcatgaa tgatggttgc gatggcgttg
     9721 tcagcagggc ccgtcgaaga aggaataaac cagccttgca gacgagtacc gtccttagcc
     9781 gtaaattcga cggattccgc cgaccagggg tcgggaccgt atattttatc gtcaggataa
     9841 tagaagaagt tgatggcgac gcgcggcact aaataaacgc agaaggcaac aacaaagaga
     9901 ataaacagaa ttttgagaac gcgtttgttc actggcagcg ccataatctt tccttgcgac
     9961 ttctttatca tccattaatc ggggaaagat ggtgggagag tgacgaaaga aagtaaagcg
    10021 gtgaggaagt atttcggaat agcgcctgaa aggcgagggc gggtgagtga tatcacccgc
    10081 ctgagtcatt aacgcttcag agcgtcgctt aactcgtcac gcatgttcgc cagcatggct
    10141 ttaacaacgc gcgggttacc agcaacgatg ttaccggtca gcatatagtt atgaccaccg
    10201 gtgaagtcgc tgacgatgcc gcctgcttca cgaaccagca gctcgcctgc cgcgaaatcc
    10261 cacgggcgca gaccgatttc aaagaaaccg tcaacacgac ccgcagcgac gtaagccaga
    10321 tccagcgccg cagaaccggt acgacggaag tctgcacatt cgttgaacag tttgccgacg
    10381 atgttgatgt aggtagtggc gtactgtttt gctttgaacg ggaagccggt cgccagaata
    10441 gtaccgtcga gatcgcgagc ggtgctgccg cgcagacggt agccgttcag ctgtgcgccc
    10501 tgaccgcgag tggcggtgaa cagttcgtta cgcataggat cgtataccac agcaacttcg
    10561 gtgcggcctt tgatacgaac ggcgatagat accgcgaagt gcggcagacg tttgataaag
    10621 ttggtagtgc catccagtgg atcgataacc cattgaacat cctgatcagt accttcaagt
    10681 tcaccgcttt cttcggtgat gatggtgtgc tgtgggtaag atttacgaat cgtgtcgata
    10741 atcaccgctt cggcagcttt atctacgttg gtcacgaaat cgttactgcc tttctggctc
    10801 gcttctacag cgtccggggt ttcatagttt ttggcaatta aattacccgc cttgcgcgct
    10861 gcgcgcacgg cgatgttcag catcggatgc atcggtctct ctcactggat gttaaagaac
    10921 gggaaaacgg cgcagagtat agcagcgaga tcggcatctg tcttcgtttt atgataagat
    10981 gcgtgaataa tctttagatg aagaaagaca atgctgcaaa atattcgaat tgtgctggtg
    11041 gagacgtcac acaccggcaa tatgggttct gttgcccgtg ccatgaaaac aatgggatta
    11101 accaatctgt ggctggttaa tccactagtg aaaccagact cccaggcgat tgccctggca
    11161 gcaggggcca gcgatgtgat tggtaatgct cacatcgtcg atacgctcga cgaagcgtta
    11221 gccggttgta gtctggtggt gggcaccagc gctcgctccc gcacgctgcc gtggccgatg
    11281 ctcgacccgc gcgaatgcgg tctgaaaagc gtcgctgaag cggcaaatac cccggtggcg
    11341 ctggtgtttg gtcgcgagcg cgtcggcttg accaatgaag agttgcagaa atgccattat
    11401 catgtcgcga ttgcggctaa cccggaatac agttcgctga acctggcgat ggcggttcag
    11461 gttattgcct atgaagtgcg catggcctgg ctggcaactc aggaaaatgg cgagcaggtc
    11521 gagcatgaag agacgccgta tccgctggtc gatgatctgg agcgttttta tggtcatctg
    11581 gagcaaacgc tgctggcaac cggttttatc cgtgaaaacc atccggggca ggtgatgaat
    11641 aaattgcgcc gtctgtttac ccgtgcacgc ccggaaagcc aggaattgaa tatcctgcgc
    11701 gggattctgg cttctattga gcagcagaat aaaggtaaca aggccgaata acagtggttg
    11761 cctgatgcga tgcttaacgc gtcttatcag gcctgtaagt gcacggaatc caaggtcgga
    11821 taaggtgttc acaccgcatc cgacaacagg tacaaacgcc acgataaaaa aatggcactg
    11881 aaggttaaat acccgactaa atcagtcaag taaatagttg accaatttac tcgggaatgt
    11941 cagacttgac cctgctatgc aataccccca attttacaat aaaaaacccc gggcaggggc
    12001 gagtttgagg tgaagtaaga catgagactg acatctaaag ggcgctatgc cgtgaccgca
    12061 atgcttgacg ttgcgctcaa ctctgaagcg ggcccggtac cgttggctga tatttccgaa
    12121 cgtcagggaa tttccctttc ttatctggaa caactgtttt cccgtctgcg taaaaatggt
    12181 ctggtttcca gcgtacgtgg accaggcggt ggttatctgt taggcaaaga tgccagcagc
    12241 atcgccgttg gcgaagtgat tagcgccgtt gacgaatctg tagatgccac ccgttgtcag
    12301 ggtaaaggcg gctgccaggg cggcgataaa tgcctgaccc acgcgctgtg gcgtgatttg
    12361 agcgaccgtc ttaccggttt tctcaataac attactttag gcgaactggt taataaccag
    12421 gaagtgctgg atgtgtctgg tcgtcagcat actcacgacg cgccacgcac ccgcacacaa
    12481 gacgcgatcg acgttaagtt acgcgcttaa taaaaagaat tcagaatcag gccggagtgc
    12541 taaacactcc gtaaacacgg tcgtacatcc agccggtagc ctggttcctt gcattgagtg
    12601 atgtacggag tttatagagc aatgaaatta ccgatttatc tcgactactc cgcaaccacg
    12661 ccggtggacc cgcgtgttgc cgagaaaatg atgcagttta tgacgatgga cggaaccttt
    12721 ggtaacccgg cctcccgttc tcaccgtttc ggctggcagg ctgaagaagc ggtagatatc
    12781 gcccgtaatc agattgccga tctggtcggc gctgatccgc gtgaaatcgt ctttacctct
    12841 ggtgcaaccg aatctgacaa cctggcgatc aaaggtgcag ccaactttta tcagaaaaaa
    12901 ggcaagcaca tcatcaccag taaaaccgaa cataaagcgg tactggatac ctgccgtcag
    12961 ctggagcgcg aaggttttga agtcacttac ctggcaccgc agcgtaacgg cattatcgac
    13021 ctgaaagaac tggaagcagc gatgcgtgat gacaccatcc ttgtttccat catgcacgta
    13081 aataacgaaa tcggcgtggt gcaggatatc gcggctatcg gcgaaatgtg ccgtgctcgt
    13141 ggcattatct atcacgttga tgcaacccag agcgtgggca aactgccgat cgatctgagc
    13201 cagttgaaag ttgacctgat gtctttctcc ggtcacaaaa tctatggccc gaaaggtatt
    13261 ggtgcgctgt atgtgcgtcg taaaccgcgc gtacgtatcg aagcacaaat gcatggcggc
    13321 ggtcacgagc gcggtatgcg ttccggcact ctgcctgttc accagatcgt cggtatgggc
    13381 gaagcctatc gcatcgcaaa agaagagatg gcgaccgaga tggaacgtct gcgcggcctg
    13441 cgtaaccgtc tgtggaacgg catcaaagat atcgaagaag tttacctgaa cggtgacctg
    13501 gaacacggtg cgccgaacat tctcaacgtc agcttcaact acgttgaagg tgagtcgctg
    13561 attatggcgc tgaaagacct cgcggtttct tcaggttccg cctgtacgtc agcaagcctc
    13621 gaaccgtcct acgtgctgcg cgcgctgggg ctgaacgacg agctggcaca tagctctatc
    13681 cgtttctctt taggtcgttt tactactgaa gaagagatcg actacaccat cgagttagtt
    13741 cgtaaatcca tcggtcgtct gcgtgacctt tctccgctgt gggaaatgta caagcagggc
    13801 gtggatctga acagcatcga atgggctcat cattaatcgg tatcggaatc aggagaattt
    13861 ataatggctt acagcgaaaa agttatcgac cattacgaga atccgcgtaa cgtgggttcc
    13921 tttgacaaca acgacgagaa cgtcggcagc ggcatggtgg gtgcaccggc ctgtggcgac
    13981 gtgatgaagt tgcagattaa agtcaacgat gaaggtatca ttgaagacgc gcgttttaaa
    14041 acttacggct gcggttccgc tatcgcttcc agctccctgg tcaccgaatg ggtgaaaggg
    14101 aagtctctcg acgaagcaca ggcgataaaa aacaccgata ttgctgaaga acttgaactg
    14161 ccgccggtga aaattcactg ttctattctg gcagaagacg cgatcaaagc cgccattgcg
    14221 gactataaaa gcaaacgtga agcaaaataa gagttgaggt ttggttatgt cgattacact
    14281 gagcgacagt gcagcagcgc gagtaaatac ctttctggct aaccgcggta aagggtttgg
    14341 cctgcgtctg ggcgtgagaa cctccgggtg ttcaggtatg gcttatgtac tggaatttgt
    14401 tgacgaaccg acgccggaag acatcgtgtt tgaagacaaa ggcgtgaaag tcgtggtcga
    14461 tggcaaaagc ctgcaatttc tggacggtac gcagctggac ttcgtcaaag aaggccttaa
    14521 cgaagggttt aaattcacca acccgaacgt caaagatgag tgtggttgcg gcgaaagctt
    14581 ccacgtttga tgcgcatacc gataacccca ccgtggtcgc ctgcgcgtgg ggtttgtttt
    14641 acctgattcg ccgttatcgc ggcggatcgc agccctgaga atgttatgga ttacttcacc
    14701 ctctttggct tgcctgcccg ttatcaactc gatacccagg cgctgagcct gcgttttcag
    14761 gatctacaac gtcagtatca tcctgataaa ttcgccagcg gaagccaggc ggaacaactc
    14821 gccgccgtac agcaatccgc gaccattaac caggcctggc aaacgctgcg tcatccgtta
    14881 atgcgcgcgg aatatttgct ttctttgcac ggttttgatc tcgccagcga gcagcatact
    14941 gtgcgcgaca ccgcgttcct gatggaacag ctggaactgc gcgaagaact ggacgagatc
    15001 gaacaggcga aagatgaagc gcggctggaa agctttatca aacgtgtgaa aaagatgttt
    15061 gatacccgcc atcagttgat ggttgaacag ttagacaacg agacgtggga cgcggcggcg
    15121 gataccgtgc gtaagctgcg ttttctcgat aaactgcgga gcagtgccga acaactcgaa
    15181 gaaaaactgc tcgattttta atttctggaa gctaaacatg gccttattac aaattagtga
    15241 acctggtttg agtgctgcgc cgcatcagcg tcgcctggcg gccggtattg acctgggcac
    15301 aaccaactcg ctggtggcaa cagtgcgcag cggtcaggcc gaaacgttag ctgaccatga
    15361 aggccgtcac ctgctgccat ctgttgttca ctatcaacag caagggcatt cggtgggtta
    15421 tgacgcgcgt actaacgcag cgctcgacac cgccaacacc attagttctg ttaaacgcct
    15481 gatggggcgc tcgctggctg atatccagca acgctatccg catctgcctt atcaattcca
    15541 ggccagcgaa aatggcctgc cgatgattga aacggcggcg gggctgctga acccggtgcg
    15601 cgtttctgcg gacatcctca aagcactggc ggcgcgggca actgaagccc tggcaggcga
    15661 gctggatggt gtcgttatca ccgttccagc gtactttgac gatgcccagc gtcagggcac
    15721 caaagacgcg gcgcgtctgg cggggctgca tgttctgcgc ttacttaatg aaccgaccgc
    15781 tgcggctatt gcctacgggc tggattccgg ccaggaaggc gtgatcgctg tttatgacct
    15841 cggcggcggg acgtttgata tttccattct gcgcttaagt cgcggcgtgt ttgaagtgct
    15901 ggcgaccggc ggtgattccg cgctcggcgg cgatgatttc gaccatctgc tggcggatta
    15961 cattcgcgag caggcgggca ttcctgatcg tagcgataac cgcgttcagc gtgaactgct
    16021 cgatgctgcc attgcggcca aaatcgcgct gagcgatgcg gactccgtga ccgttaacgt
    16081 tgcgggctgg cagggcgaaa tcagccgtga acaattcaat gaactgatcg cgccactggt
    16141 aaaacgaacc ttactggctt gtcgtcgcgc gctgaaagat gcgggcgtag aagctgatga
    16201 agtgctggaa gtggtgatgg tgggcggttc tactcgcgtg ccgctggtgc gtgaacgagt
    16261 aggcgaattt ttcggtcgtc caccgctgac ttctatcgac ccggataaag tcgtcgctat
    16321 tggcgcggcg attcaggcgg atattctggt gggtaacaag ccggacagcg aaatgctgtt
    16381 gctggatgtg atcccgctgt cgctgggcct cgaaacgatg ggcggcctgg tggagaaagt
    16441 gatcccgcgt aataccacga ttccggtggc ccgcgcccag gatttcacca cctttaaaga
    16501 tggtcagacg gcgatgtcta tccatgtgat gcagggtgag cgcgaactgg tgcaggactg
    16561 ccgctcgctg gcgcgttttg cgctgcgagg tattccggcg ctaccggctg gcggtgcgca
    16621 tattcgcgtg acgttccagg tcgatgccga cggtcttttg agcgtgacgg cgatggagaa
    16681 atccaccggc gttgaggcgt ctattcaggt caaaccgtct tatggcctga ccgatagcga
    16741 aatcgcctcg atgatcaaag actcaatgag ctatgccgag caggacgtaa aagcccgaat
    16801 gctggcagaa caaaaagtag aagcggcgcg tgtgctggaa agtctgcacg gtgcgctggc
    16861 tgctgatgcc gcgctgttaa gcgccgcaga acgtcaggct atcgacgatg ctgctgctca
    16921 cctgagtgaa gtggcgcagg gcgatgatgt tgacgccatc gaacaagcga ttaaaaacgt
    16981 agacaaacaa acccaggatt tcgccgctcg ccgcatggac cagtcggttc gtcgtgcgct
    17041 gaaaggccat tccgtggacg aggtttaata tgccaaagat tgttattttg cctcatcagg
    17101 atctctgccc tgatggcgct gttctggaag cgaatagcgg tgaaaccatt ctcgacgttg
    17161 cgctgcgtaa cggtatcgag attgaacacg cctgtgaaaa atcctgtgct tgcaccacct
    17221 gccactgcat cgttcgtgaa ggttttgact cgctgccgga aagctcagag caggaagacg
    17281 acatgctgga caaagcctgg ggactggagc cggaaagccg tttaagctgc caggcgcgcg
    17341 tcaccgacga agatttagtg gttgaaatcc cgcgttacac tatcaaccat gcgcgtgagc
    17401 attaacagag gttagtatgg gacttaagtg gaccgatagc cgcgaaattg gcgaagcact
    17461 gtacgatgcg tatcctgata ttgatccgaa aacggttcga ttcaccgata tgcatcagtg
    17521 gatttgcgct ctggaagatt tcgacgacga cccgcaggca tcgaacgaga aaatcctcga
    17581 agcgattttg ttggtctggc tggacgaagc cgagtgaatg aacgggctgc ctgggggtag
    17641 cccgttttgc tgttaaacga ataaggataa ctaaaatgac agaagcgatg aagattaccc
    17701 tctctcccca acctgccgac gcacgttggg gagaaaaagc gacttacagc attaataatg
    17761 atggcattac cctgcatttg aacgggacag acgatctggg gttgatccag cgtgcggcgc
    17821 gcaaggttga cggtctgggc attaagcatg ttcagttaag cggtgaaggt tgggatgcgg
    17881 atcgctgctg ggcattctgg caaggttaca aagccccgaa aggcatacgt aaagtggagt
    17941 ggccggatct ggacgatgcc cagcgtcagg aactggataa ccgcctgatg atcatcgact
    18001 gggtgcgtga caccatcaac gcaccggcag aagagctggg gccatcgcaa ctggcacagc
    18061 gtgctgttga tctgatcagc aacgtcgcag gggatcgtgt gacttaccgg atcaccaaag
    18121 gcgaagatct gcgtgagcaa ggttatatgg ggctgcacac tgtcgggcgc ggttcagaac
    18181 gttctccggt attgctggcg ctggattaca acccaactgg cgataaagaa gcgccagtgt
    18241 acgcgtgcct ggttggtaaa ggcatcactt ttgattccgg cggctacagc atcaaacaga
    18301 ccgcgtttat ggactcgatg aagtcggaca tgggcggcgc ggcaacggtt accggggcgc
    18361 tggcatttgc cattacgcgc ggactgaaca agcgcgtgaa gctgttcctc tgttgtgcgg
    18421 ataacctgat cagcggcaac gcgttcaaac tgggcgatat tattacctat cgcaacggta
    18481 aaaaagttga agtgatgaac actgatgccg aagggcgtct ggtgctggcc gatggcctga
    18541 ttgatgccag tgcgcagaaa ccggaactga tcattgatgc ggcgaccctc accggggcgg
    18601 cgaaaaccgc gctgggtaat gattatcacg cgctgttcag ttttgatgat gcgctggcag
    18661 ggcgcttgct ggcgagtgcc gcgcaggaga atgaaccgtt ctggcgtctg ccgctggcgg
    18721 agttccaccg cagccagctg ccgtctaact ttgcggaact gaacaatacc ggaagcgcgg
    18781 cgtatccggc aggcgcgagc acggcggcgg gcttcctgtc gcactttgtt gagaactatc
    18841 agcaaggttg gctgcatatc gactgctcgg cgacttaccg taaagcgccg gttgaacagt
    18901 ggtctgcggg tgctacggga cttggtgtgc gcacgattgc taatctgtta acggcgtaaa
    18961 ctaatatgcc ggatgcggcg taaacgcctt atccggccta caaagtattg caaattcaat
    19021 atattgcggt tcccttgtag gcctgataag cgtagcgcat caggcactta cgccccgaac
    19081 gttgaatgaa ttatggttct tatgtccgaa acaaaaaacg aacttgaaga cctgctggaa
    19141 aaagcagcaa ccgaaccggc gcaccgcccg gcctttttcc gtactctact ggaatccacc
    19201 gtctgggtgc ctggtacggc ggcacagggc gaggctgtgg ttgaagatag cgcacttgat
    19261 ttacagcact gggaaaaaga agacggcacc agcgtcattc cttttttcac ctcgttagaa
    19321 gcacttcaac aggcggttga aggcgaacag gcatttgtcg taatgccagt tcgcacattg
    19381 tttgagatga cccttggcga aacgctcttc cttaatgcca aactgccaac cggtaaagaa
    19441 tttatgccgc gtgaaatcag tttgctgatt ggtgaagaag ggaatccgct tagcagtcag
    19501 gaagtactgg aaggcggtga atcgctgata ttatcggagg tcgcagagcc gccagcgcaa
    19561 atgattgatt cacttaccac cttatttaaa accattaagc cggtgaagcg tgcttttatt
    19621 tgttcaatta aagagaacga agaggcacag cctaatttac ttattggcat tgaagccgat
    19681 ggtgatatcg aagaaattat tcaggcggcg ggaagtgtag cgaccgatac attacctggc
    19741 gatgaaccca tcgatatttg tcaggtgaaa aaaggggaaa aaggtattag ccactttatt
    19801 accgaacata ttgcgccatt ctatgaacgt cgctggggtg gttttttgcg tgactttaaa
    19861 cagaaccgga taatttaaaa catgccgccc caaagggcgg cattcgggct tacttgctgt
    19921 tctgaaggtt gttaaccacc agtaaattgt tgccggagag actccggtta ttatttacct
    19981 cggttaacag cgcaccagaa acaataagta cgatacaaaa gcaccgtatc ttcattgtgc
    20041 ggtccatgcg aagaccgcat cacgacagca ccactggcga tattgccgcg atagcaagcg
    20101 ctgagacgcg cttgccctaa cggtcgcgga acatcctttc cttaagccag cgcccgcttg
    20161 cgagacggcg gaaagggccg cagtggataa tacactaaca gaacaataat aactctcttt
    20221 tacttgacaa aaagagagtt actggcgagt attgtgtccg ctgagacgcg cttgcattac
    20281 tacggtaata caacctgctg gaaaggccgc gaaccagacc agcaataaaa aaccgccaaa
    20341 ttcggcggtt ttttattgcc tgtaaaaagc ttattttacg ggctcaaccg gtaaatctgc
    20401 ccgtgcgccc cattcactcc atgcgccgtc gtacagtttc acgtttgtca catccagcgt
    20461 cgcaagtgcc aacaaaacca cggctgctgt tacaccagaa ccgcagctga cgataattgg
    20521 tttgtcgtag ctgacaccgc gaccaaaaaa tatcgcatcc agttcatcgg tcgtttttag
    20581 ttcgccttca cgtaccagtt ccgtccacgg aacgttcaat gcaccgggaa tatgtccgcg
    20641 acgtaaacct gggcgaggtt catcaacttc tgcgttaaaa cgtgtagccg ggcgggcatc
    20701 aataatttgc gccgtatttt catggcttgc caataataca tcggttactt tcaccacggc
    20761 ttcaggatta aacgcggcgt taaactctcc ttccggcagc tctactgcgc cttcctctaa
    20821 tagcagatca tcacgctgcc agcctgcaag tccaccaccc agaatcgaca ctttctctac
    20881 accaaaggtg cgcagcatcc accatgctcg tggggctgag aaaagattac cttcgtcata
    20941 gacaatcagg tgcttatcct ggttaacgcc taattcacgc atcgccacgg cgaacgtttc
    21001 cgggcgcggc agcatgtgcg gaagcggaga agtgtgatca gaaagcgctt cgatatcaaa
    21061 aaacactgcg ccgggaatat gtccattcag atactcctga gcaacgttac gatcttcctg
    21121 tccaggcgac gccatgcggg catcaataat ctgaatttcc gggtcatcaa tatgttcggc
    21181 gagccagtcg gctcctacaa accatgtcgt ggacataggc atctccattt ttgccggggt
    21241 taggctaaat tgtcggatgt ttagtgaaaa gcgacaagca aaagaggctg atttggctgt
    21301 ctgagccaca attctcacta aaattcggat tgtatgggcg tgatactgaa ttacataacg
    21361 taaattctca cgcataataa ttattcgacg ttgctaacgg gccattgtgg cagggatgaa
    21421 aaatgaaaaa gttacgcgta gccgcctgca tgctaatgct ggcgctggca gggtgcgaca
    21481 acaacgataa cgcgccaaca gcggtgaaaa aagatgcgcc ttctgaagtt actaaagcgg
    21541 cctcttcaga aaacgtgagt tcagcaaaac tttccgcgcc ggagcgacaa aaactggccc
    21601 aacagagtgc cggtaaggcg ctgacattgc tggatctctc tgaagttcaa ctcgatggcg
    21661 cagccacgct ggtgctgacg ttctccatcc ctctcgaccc ggatcaggat ttctcacgcg
    21721 ttattcatgt cgtcgataaa aaaagcggca aagtggatgg tgcctgggag ctgtcacata
    21781 atcttaaaga gctgcgttta cgccacctcg aaccgaaacg tgatttgatc gtcactattg
    21841 gcaaggaggt caaagcgctc aataacgcaa cctttagtaa agatgacgaa aaaactataa
    21901 ctacccgcga tatccaaccc agcgtcggtt ttgccagtcg tggttcgttg cttcctggta
    21961 aggtcgttga agggctgccg gtaatggcgc ttaacgttaa caatgtcgat gttaacttct
    22021 tccgtgtgaa accagaatca ctgcccgctt tcattagcca gtgggaatac cgcaattcgc
    22081 tggcgaactg gcagtcagat aaactgctgc agatggcgga tctggtctac accggacggt
    22141 ttgatctcaa tcctgcgcgt aacacccgtg aaaaattatt gctgccgctg ggcgatatca
    22201 aaccgcttca gcaggcgggc gtgtatctgg ctgtgatgaa tcaggctgga cgttacgatt
    22261 acagtaatcc cgcgacgctg tttacgttaa gtgatattgg cgtttcagct caccgttatc
    22321 acaatcgtct ggatatcttt acccaaagtc tggaaaacgg cgcggcccag caaggaattg
    22381 aagtctcttt attaaatgag aaagggcaga ctctgactca ggcaagcagt gacgctcagg
    22441 ggcatgtgca gctggaaaat gataaaaacg cggcattact gctggcgcgt aaaaacggtc
    22501 agacaacgct gctcgattta aaactcccgg cgctggactt agcggaattt aacattgctg
    22561 gcgcgccggg ctatagcaaa cagtttttca tgtttggccc gcgcgatctt tatcgcccgg
    22621 gtgaaacggt catcctcaat ggtttgctgc gtgatgcaga cggtaaagcg ttgcccgatc
    22681 aacctgtcaa gctggacgtg attaaacccg acgggcaggt actcaggagc gtcgttagtc
    22741 agccggagaa tggcctctac cactttacct ggccactcga tagcaatgcg gcaaccggta
    22801 tgtggcatat tcgcgctaac acgggcgata accagtatcg gatgtgggat ttccacgtcg
    22861 aagattttat gccagagcgt atggcgctga atctgaccgg tgagaaaacc ccgctaacgc
    22921 cgaaagatga agtgaaattc tccgtggtgg ggtactacct gtatggcgca cctgctaatg
    22981 gtaatacttt gcaagggcaa cttttcctgc gcccactgcg tgaagctgta tcagccttac
    23041 ctggttttga attcggcgat atagctgccg aaaatctttc ccgcacgctg gatgaagttc
    23101 agttgacgct ggatgataaa gggcgcggcg aagtttctac agaaagccag tggaaggaaa
    23161 cgcattcccc attacaggtt attttccagg gtagtttgct ggaatcgggc ggtcgcccgg
    23221 tgacgcgccg tgctgagcag gctatctggc ctgccgatgc attgccgggg atccgtccgc
    23281 agttcgcctc gaaatcggtt tacgattatc gcaccgacag cacggtgaaa cagcccattg
    23341 ttgatgaagg cagtaacgcc gcttttgaca tcgtttatag cgatgcgcaa ggcgtgaaaa
    23401 aagccgtgtc gggcttgcag gtgcgcctga ttcgcgaacg ccgcgattac tactggaact
    23461 ggtcggaata tgaaggctgg cagtcacagt ttgatcaaaa agatctgatc gaaaatgaac
    23521 aaactctgga tctgaaagcg gacgaaaccg gtaaggtcag tttcccggta gagtggggcg
    23581 cttatcgtct ggaagttaaa gcgccgaatg aagcggtcag tagtgtgcgt ttctgggctg
    23641 gttatagctg gcaggataac agcgacgggg gtggtgcagt gcgacccgac cgtgtcacgc
    23701 tgaaactgga taaagccagc tatcgccctg gcgataccat taagttgcat attgccgcgc
    23761 caacggcggg taaaggttat gcgatggtcg agtccagtga agggccgctg tggtggcaag
    23821 agattgatgt tccggctcaa gggctggatc tgacgattcc ggtcgataaa acctggaatc
    23881 gtcatgatct gtatttaagt acgctggtgg tacgtcctgg cgataaatct cgctccgcga
    23941 cgccaaaacg cgcggttggg gtgttgcatc tgccgcttgg tgatgaaaac cgtcgcctcg
    24001 atctggcgct ggaaacccct accaaaatgc gccccaatca gccattaacc gtgaaaatta
    24061 aagccagcaa taaaaatggt gagatgccaa aacaggtgaa tgtgctggtg tctgccgttg
    24121 atagcggtgt gctgaatatt actgattacg tcacgccaga tccgtggcag gcgttctttg
    24181 gtcagaaacg ctatggcgca gacatttacg atatttacgg tcaggtcatt gaaggtcagg
    24241 ggcgtctggc agctctgcgt tttggtggcg atggtgatga gctgaaacgt ggtggtaaac
    24301 cgccggtcaa tcacgtcaat attgtcgcgc agcaggcgct gccggtaacg ctcaatgaac
    24361 agggcgaagg ctcggttaca ctgccaattg gcgattttaa cggtgaattg cgcgtcatgg
    24421 cgcaagcctg gacggcagat gacttcggaa gcaacgaaag caaagtgatt gttgctgcac
    24481 cggtgattgc tgaactgaac atgccacgct ttatggcgag tggcgatacc tcgcgcctga
    24541 cgctggatat cactaatctt accgataaac cgcaaaaact taatgttgct ctgaccgcca
    24601 gtggcttgct tgaactggtg agtaattccc ccgcacctgt tgaattagcg ccaggggtgc
    24661 gtactacgct gtttatcccg gtgcgtgcat tgccgggtta tggcgatgga gacattcagg
    24721 ccaccattag cgggttagcg ttaccggggg aaaccgttgc cgatcagcat aagcagtgga
    24781 aaatcggcgt ccgtccggca ttcccggcac aaacggtcaa ttacggtacg gcgttacagc
    24841 ctggtgaaac ctgggcgctc ccggctgatg gattgcaaaa cttctcgcct gttacgctgg
    24901 aagggcaatt gttgttgagt ggcaaaccac cgctgaacat cgcacgttat atcaaagagt
    24961 taaaagcgta tccgtacggc tgtctcgagc aaaccgccag cggcctgttc ccgtcacttt
    25021 ataccaatgc agcccaactg caggcgttgg gcatcaaagg cgacagtgat gagaaacgcc
    25081 gtgcatcggt cgatatcggc atttcccgtt tgctgcaaat gcaacgtgat aacggcggct
    25141 ttgcgttgtg ggataaaaac ggtgacgaag agtactggct gacggcttac gtgatggatt
    25201 tcctggtccg cgcaggcgaa cagggttaca gcgtgccgac agacgccatt aaccggggta
    25261 atgagcgtct gctgcgctat ttacaagatc cgggcatgat gtcgatcccg tacgctgata
    25321 atctcaaagc cagtaaattc gccgtacagt cttacgctgc gctggtgttg gcccgtcagc
    25381 aaaaagcacc actgggtgcg ctgcgtgaaa tctgggagca tcgtgcagat gccgcttctg
    25441 gtttaccgct gctgcaactt ggcgttgcgc tgaaaatcat gggtgatgcg acgcgtggtg
    25501 aagaagcgat tgcgctggcg ctgaaaacgc cgcgtaatag tgatgagcgg atatggctgg
    25561 gtgattacgg tagtccactg cgagacagcg cgttgatgct ctcattgctg gaagagaaca
    25621 aactgctacc cgatgagcag tactccctgt taaacacact ttcgcagcag gcgttcggtg
    25681 aacgctggct atcgacgcag gaaagtaacg cgttgttcct ggctgcccgt actcttcagg
    25741 atttaccggg caaatggcag gcgcaaacca ctttctctgc tgagccgttg acaggcgaga
    25801 aagcgcaaac cagcaatctg aatagcgatc aacttgccac cttgcaggtg accaacagtg
    25861 gcgatcagcc gttatggctg cgtgtggatg ccagcggtta tccgcaatcc gcacctttac
    25921 cggcgagcaa tgtgctgcaa attgagcgtc atattctggg tactgacggt aagagcaaat
    25981 cgctggactc gttacgtagc ggcgatctgg tgctggtgtg gttgcaggtt aaagccagta
    26041 acagtgtacc ggatgcgtta gtcgtggatc tgttgcctgc ggggctggaa ctggaaaacc
    26101 agaatctggc gaacggcagc gccagcctgg agcaaagtgg tggtgaagtg cagaacttgc
    26161 tgaaccagat gcagcaggcg agcattaagc acatagagtt ccgtgacgat cgctttgtgg
    26221 cggcggttgc cgttgatgaa tatcaaccgg taacgctggt gtatctggcg cgggcggtga
    26281 cgccgggaac gtatcaggta ccgcaaccga tggtggaatc aatgtatgtt ccccaatggc
    26341 gggcgaccgg cgcggctgaa gatttgctga ttgtcagacc gtaaatgcct cgcctgttaa
    26401 ccaaacgcgg ctgctggata atgctggcag ccgcgccttt tatcatcatt ctggcagcat
    26461 gggcagcaga taaactctgg cctctgccat tgcaagaagt gaatcctgca cgcgtggtgg
    26521 tggcgcagga tggtacgcca ctctggcgtt tcgccgatgc tgacggcatc tggcgttatc
    26581 cagtaacaat cgaagatgtt tctccacgtt accttgaagc gctgatcaat tatgaagatc
    26641 gctggttctg gaagcatccg ggggtgaatc cgttctcggt ggcgcgtgca gcctggcaag
    26701 atctcacgtc gggtcgggtc atttccggcg gcagcacgct caccatgcag gtggcgcgcc
    26761 tgcttgatcc tcaccccaaa acgtttggcg gcaaaattcg ccagctctgg cgcgcgttgc
    26821 aactggaatg gcatctgtct aaacgtgaaa ttctgacctt gtatcttaac cgtgccccgt
    26881 ttggcggtac gttgcagggg attggtgcgg caagttgggc ttacctcggg aaatcgcctg
    26941 cgaatttaag ctattccgag gcggcaatgc tggcggtgtt gccgcaagcg cccagccgtc
    27001 ttcgcccgga tcgttggccg gagcgtgccg aagccgcgcg taataaagtg ctcgaacgga
    27061 tggccgtgca aggtgtgtgg tcccgtgagc gggtaaaaga gtcaagggaa gaacccatct
    27121 ggctggcacc ccgacaaatg ccgcaactgg caccgctgtt ttcgcgaatg atgctcggta
    27181 aaagcaaaag cgacaaaatc gttactacgc tggatgccgg tcttcaacga cgtctggaag
    27241 aactggcgca aaactggaaa ggacgattgc caccgcgcag ctcactggcg atgatcgtgg
    27301 ttgatcatac cgatatgcgt gttcgcggct gggtgggatc ggttgatctc aacgatgatt
    27361 cacgctttgg tcatgttgat atggtcaatg cgatccgatc gccaggatcg gtgctcaaac
    27421 cgtttgttta tggtctggcg ctggatgaag gcttgatcca cccggcatcg ctgttgcaag
    27481 acgtccccag acgcaccggt gattaccgac ctggcaactt tgacagcggt tttcatggcc
    27541 cgatcagcat gagtgaggcg ctggtgcgat cgctgaactt acctgctgtg caggtgctgg
    27601 aagcctatgg accgaaacgg tttgcggcaa agttacgcaa tgttggattg ccgttatatt
    27661 tgcccaacgg tgctgcaccg aatctttcgc tcattctcgg cggcgctggc gcaaaactgg
    27721 aagatatggc ggcagcgtat accgcgtttg ctcgccacgg caaggcaggt aaattgcgct
    27781 tacagcctga cgatccgctg cttgaacggc ctttaatgtc gtcgggggct gcgtggatca
    27841 ttcgccggat tatggctgat gaagcgcaac ccttgccgga tggtgccttg ccgcgcgtcg
    27901 ccccactggc atggaaaacg ggcaccagct atggctatcg tgatgcctgg gcgattgggg
    27961 ttaacgctcg ctatgtcatt gggatctgga ctggcagacc ggacggcacg cccgttgttg
    28021 gtcagtttgg ctttgccagt gccgtaccgc tgttgaatca ggttaataat atcttactgt
    28081 cgcgcagtgt gaatttgccg gaagacccac gtcctgactc tgtaagtcgt ggcgttatct
    28141 gctggccggg cggacagtct ttgccggaag gtgacggtaa ctgtcgccgc cgcctggcaa
    28201 cctggctgct ggacgggagc cagccgccaa ctctattact gccggagcag gaaggcatta
    28261 atggcattcg tttccccatc tggctggatg aaaacggcaa acgtgttgcc gctgattgcc
    28321 ctcaggcgcg acaagaaatg ataaatgtct ggccgctacc gctggaaccc tggctgcccg
    28381 catcagaacg tcgcgctgtg cgcttgccac cggcctcgac aatttgcccg ccatacggtc
    28441 acgatgcaca actcccgctg cagctaacag gtgtgcgcga tggcgcgatt attaaacgtt
    28501 taccgggcgc agcggaagcg actttgccgt tgcaatcaag tggaggggca ggtgaacgct
    28561 ggtggtttct gaacggcgaa ccgttaactg aacgtgggcg caacgtgacc ctgcatttga
    28621 cggataaagg cgattatcaa ttgctggtga tggatgacgt ggggcaaata gcgacagtga
    28681 aatttgtcat gcaatagtct gttttgtccc tgattgttgc ttaaactggg ttta
//
LOCUS       DAZFRG010000034        28649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFRG010000034 DAZFRG010000000
VERSION     DAZFRG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.034"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..476)
                     /locus_tag="ACHKUC_001167"
     CDS             complement(<1..476)
                     /locus_tag="ACHKUC_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6617454.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPKDNHGARWLLGP"
     gene            complement(732..1997)
                     /locus_tag="ACHKUC_001168"
     CDS             complement(732..1997)
                     /locus_tag="ACHKUC_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6617455.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            complement(2195..2270)
                     /locus_tag="ACHKUC_001169"
     tRNA            complement(2195..2270)
                     /locus_tag="ACHKUC_001169"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2235..2237),aa:Phe,seq:gaa)
     gene            complement(2376..3083)
                     /gene="yqgA"
                     /locus_tag="ACHKUC_001170"
     CDS             complement(2376..3083)
                     /gene="yqgA"
                     /locus_tag="ACHKUC_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO6617456.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3476..5611
                     /gene="speC"
                     /locus_tag="ACHKUC_001171"
     CDS             3476..5611
                     /gene="speC"
                     /locus_tag="ACHKUC_001171"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO6617457.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            complement(5660..6916)
                     /gene="nupG"
                     /locus_tag="ACHKUC_001172"
     CDS             complement(5660..6916)
                     /gene="nupG"
                     /locus_tag="ACHKUC_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO6617458.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7118..8197)
                     /gene="mltC"
                     /locus_tag="ACHKUC_001173"
     CDS             complement(7118..8197)
                     /gene="mltC"
                     /locus_tag="ACHKUC_001173"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO6617459.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8262..8537)
                     /gene="yggX"
                     /locus_tag="ACHKUC_001174"
     CDS             complement(8262..8537)
                     /gene="yggX"
                     /locus_tag="ACHKUC_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO6617460.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8565..9617)
                     /gene="mutY"
                     /locus_tag="ACHKUC_001175"
     CDS             complement(8565..9617)
                     /gene="mutY"
                     /locus_tag="ACHKUC_001175"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO6617461.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAVLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQISADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9778..10497
                     /gene="trmB"
                     /locus_tag="ACHKUC_001176"
     CDS             9778..10497
                     /gene="trmB"
                     /locus_tag="ACHKUC_001176"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO6617462.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10497..10823
                     /gene="yggL"
                     /locus_tag="ACHKUC_001177"
     CDS             10497..10823
                     /gene="yggL"
                     /locus_tag="ACHKUC_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO6617463.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKMEEVRTSEL
                     FDVWWD"
     gene            11007..11726
                     /gene="yggN"
                     /locus_tag="ACHKUC_001178"
     CDS             11007..11726
                     /gene="yggN"
                     /locus_tag="ACHKUC_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO6617464.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11902..12948
                     /gene="ansB"
                     /locus_tag="ACHKUC_001179"
     CDS             11902..12948
                     /gene="ansB"
                     /locus_tag="ACHKUC_001179"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO6617465.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13065..14072
                     /gene="yggM"
                     /locus_tag="ACHKUC_001180"
     CDS             13065..14072
                     /gene="yggM"
                     /locus_tag="ACHKUC_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO6617466.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQRTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(14137..15273)
                     /gene="hemW"
                     /locus_tag="ACHKUC_001181"
     CDS             complement(14137..15273)
                     /gene="hemW"
                     /locus_tag="ACHKUC_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO6617467.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLNNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EAVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(15266..15859)
                     /gene="rdgB"
                     /locus_tag="ACHKUC_001182"
     CDS             complement(15266..15859)
                     /gene="rdgB"
                     /locus_tag="ACHKUC_001182"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO6617468.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15867..16157)
                     /gene="yggU"
                     /locus_tag="ACHKUC_001183"
     CDS             complement(15867..16157)
                     /gene="yggU"
                     /locus_tag="ACHKUC_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO6617469.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(16154..16720)
                     /gene="yggT"
                     /locus_tag="ACHKUC_001184"
     CDS             complement(16154..16720)
                     /gene="yggT"
                     /locus_tag="ACHKUC_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO6617470.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFVKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16738..17442)
                     /gene="yggS"
                     /locus_tag="ACHKUC_001185"
     CDS             complement(16738..17442)
                     /gene="yggS"
                     /locus_tag="ACHKUC_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO6617471.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRYFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17460..18440
                     /gene="yggR"
                     /locus_tag="ACHKUC_001186"
     CDS             17460..18440
                     /gene="yggR"
                     /locus_tag="ACHKUC_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO6617472.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            complement(18614..19030)
                     /gene="ruvX"
                     /locus_tag="ACHKUC_001187"
     CDS             complement(18614..19030)
                     /gene="ruvX"
                     /locus_tag="ACHKUC_001187"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO6617473.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKSKVDSASAVIILESYFEQGY"
     gene            complement(19030..19593)
                     /gene="yqgE"
                     /locus_tag="ACHKUC_001188"
     CDS             complement(19030..19593)
                     /gene="yqgE"
                     /locus_tag="ACHKUC_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO6617474.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19702..20652)
                     /gene="gshB"
                     /locus_tag="ACHKUC_001189"
     CDS             complement(19702..20652)
                     /gene="gshB"
                     /locus_tag="ACHKUC_001189"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO6617475.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTHTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(20665..21396)
                     /gene="rsmE"
                     /locus_tag="ACHKUC_001190"
     CDS             complement(20665..21396)
                     /gene="rsmE"
                     /locus_tag="ACHKUC_001190"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO6617476.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21476..22183)
                     /gene="endA"
                     /locus_tag="ACHKUC_001191"
     CDS             complement(21476..22183)
                     /gene="endA"
                     /locus_tag="ACHKUC_001191"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO6617477.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(22278..22775)
                     /gene="yggI"
                     /locus_tag="ACHKUC_001192"
     CDS             complement(22278..22775)
                     /gene="yggI"
                     /locus_tag="ACHKUC_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO6617478.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22852..24246)
                     /gene="galP"
                     /locus_tag="ACHKUC_001193"
     CDS             complement(22852..24246)
                     /gene="galP"
                     /locus_tag="ACHKUC_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO6617479.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITSSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24683..25837)
                     /gene="metK"
                     /locus_tag="ACHKUC_001194"
     CDS             complement(24683..25837)
                     /gene="metK"
                     /locus_tag="ACHKUC_001194"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO6617480.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25893..26144
                     /gene="yqgD"
                     /locus_tag="ACHKUC_001195"
     CDS             25893..26144
                     /gene="yqgD"
                     /locus_tag="ACHKUC_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO6617481.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTQFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            26255..26398
                     /gene="yqgG"
                     /locus_tag="ACHKUC_001196"
     CDS             26255..26398
                     /gene="yqgG"
                     /locus_tag="ACHKUC_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO6617482.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26492..26623
                     /gene="yqgB"
                     /locus_tag="ACHKUC_001197"
     CDS             26492..26623
                     /gene="yqgB"
                     /locus_tag="ACHKUC_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO6617483.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26632..28608
                     /gene="speA"
                     /locus_tag="ACHKUC_001198"
     CDS             26632..28608
                     /gene="speA"
                     /locus_tag="ACHKUC_001198"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO6617484.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
BASE COUNT         7046 a         7140 c         7529 g         6934 t
ORIGIN      
        1 tccggtccca gtagccagcg tgcaccatga ttatctttgg gattgtaatc cagtattttt
       61 tcaatgagca tgatggcatc ctgatgacgc tgtaaatgtg catttgccag gattgccgca
      121 tagagggcac gaagaaaagg tcggttgtca ggatgtatcc agatgattct gccactgaaa
      181 ccttcaggga taagacgatt gccaattgcc agccctttaa gggcagcatt cagcgcctta
      241 cgcggggcat tctgctccag aaatacgtat gccaagtgag cgtgtacatc aataaagtca
      301 ttctcctgtc gggcaaggcg cctgagtttg ttgatgtaac ttgtttcgct gatttcatcc
      361 gcatcgtatg catcaatcag ttcttcaaac tcatccagca acgagccaaa ccaggtttcc
      421 ggaaatatga aacagccctg gttatcgttc acttcaaagc gtaatttgcc agtcatattc
      481 tgaacctgta aaaaaggatg gaccataacc tgcaggctat aaaaattgtg atgcctggca
      541 tcggtgtcct tttattgtcc gggattaacg ttgcccatga taatacagtg aattctgttc
      601 tgtggtaaga cgttgagtta ttgaatgaga acctcagcct ggctgatggt tccgccggca
      661 taaaaaagaa attgattgag gcgggtgctc ttccccttaa gaggaagagc atgtgtgtaa
      721 atggcgttca gtcacctttt cagtggattg ttaacttttg catattcaaa cggactgata
      781 cactcctccc tgtttgcatc cagaaaatct gcccaccatt gcagcataag ccgacgttct
      841 tccagatgtt ctgctttatg aatatacgcg gcacgtactc cgttgcgctc ctgatgactc
      901 atctgacgtt caaccgcatc tcttgaccat aagccggatt caatcaatgc actgcacgcc
      961 atcgcccgga atccatggcc acagacttcc ttggttgtgt cataccccat cactctgagg
     1021 gccttattta cggtattttc actgattggt ttacgtgggt cgtgtgctcc cgtaaagatc
     1081 agaccatttt cacctgccca tgtctgaagt tctgccagta tggctacagc ctgttggctg
     1141 agtggcacaa gatgtggtgt tcgcatcttg gagccccggt atgaaaattt tacgcctggc
     1201 agaggttcgc gttcggctgg aatagtccac atggcattgt caatatcaat ttcagaccag
     1261 cgggcatatc tgagttcact ggaacgaata aaaaccagta aattcagcat aactgccagt
     1321 cgggtgacag gctggccttt ataaccgtca atacgcgaca gaagttcagg caggcgtgaa
     1381 agatccagag cagggcgatg ctggcgtttt accgtggtca atgcaccagc catatccaga
     1441 gccgggttat atctgatgat gccagactgt acggcataac gcattacagc agatattctt
     1501 tgctgcagac gactggcgat ttcataaatt tgtttaactt ctgcagcacg aacagggata
     1561 agcagatccg gggtactgag tgttgtgata tcccggttgc cgataaaggg gaaaacgtga
     1621 gtctcgaggc ttttgagtac tttatcgctg tgtgattgtg cccattgttt attactggca
     1681 tgccagcgtc gtgcaatatg ttcaaaagag agctgtaaat cagggactac ttttttagct
     1741 cttttctgtt cgcaagggtc aaccccctcc gaaatcagtt ttcgggcctc atcccgtttt
     1801 gtacgagctt cggagagaga aacttccgga tacacaccaa gtgccagggt tttctcctta
     1861 cccagaatac gataacggag acgccagtaa cgggaaccat taggatgtac cagcaggaac
     1921 atgccagccc cgtcagtgag tttataagcc ttgtcagtgg gctttgcagc ccggattttt
     1981 gcgtcagtca gtgccatagt tcagccttct gaatgcgtta aagagggtac aaattctgtc
     2041 gaactggggt atacccgcaa ttatacccac acaatggggt tgatgtgggg ggaatctggt
     2101 tgagttcggt agaattgatt tggaggcaga acgcttaaat tgtggcgttc tgaaacgaaa
     2161 aacggacctc cgtggaggtc cgttgatatg aacttggtgc ccggactcgg aatcgaacca
     2221 aggacacggg gattttcaat cccctgctct accgactgag ctatccgggc aacggggcgc
     2281 attaaacctg attcgcctcg tctcgtcaat caaatttctt caatttactg cagactgcac
     2341 aatctatcat cactttgccg ctaatgcacg cattgtcagg caaaccaggc ggtccaggcg
     2401 gcggaaaggg gcattgccag aaggagtgct ggaagcatgt tgaccaccgg gaacatttta
     2461 atgccacaga tgcgtaatcc ggttgccagc agcaataaac cgcctacggc gctgaagtct
     2521 gccatcatcg acggtgtggt cagcggtaat atcagcgcgg cagcccacgc cagcgttaac
     2581 tggatgatca gtaatgggat actaatcacc gataccgcaa tacccagcga gcaggcgaag
     2641 atcatcgccg taaagaaatc aagaaatgac ttggcgatta aaatactcgg atcgccggtc
     2701 atcccttcgt tcatcgcccc gaagatcccg gtgccgctgg cgcaaaacag gacaataatc
     2761 gcgacataat tctgaataaa agattcatgc gctggcttct tacgtgagtg gcgaaacaga
     2821 ttttgtgctt tggtgaccgc ggtattgacg cctttttcca gcagacaaat ttcgccgatt
     2881 aatgcaccga gtaaggttgc taaaaccatc gccggaaggt tggcgcattt caccaccagt
     2941 aaaataccaa tccccagcga tgccagacca aaaattgatg tcatggagac gcggatacgt
     3001 tccggtaagc gttggctgag cagtgcgccg agaacgccac ccagtaagac tgcactagcg
     3061 ttgataaaag ggccgatgac cacaagagtt cctgttaact gtattgtttg attttcttat
     3121 tatgaacttt ttgccttgtt gatggcttgt ctggtcgcaa agtcctggct tgcacgcttt
     3181 agcgaaaggt gccatgattg cgcgaatttt ctcctctctg tacggagttt gcccgatgca
     3241 cgccacctcc ttacattctc tcgcttatcg ccgtttcgcg cgaaaatcct ccctttttct
     3301 gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc agagcgtttc
     3361 tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata tctcgtgggg
     3421 cagggttttt caccttgtcc ggtattctta cttccccgaa acgggttttg cgcttatgaa
     3481 atcaatgaat attgccgcca gtagtgaact ggtatcccga ctttctactc atcgccgcgt
     3541 ggtggcgttg gcagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga
     3601 tagtcgcagt ggcattcttg cgttgcttaa gcgcaccggt tttcatctac cggtgttttt
     3661 gtattccgaa catgttgttg aattacctgc gggcgttacg gcggtaatca acggcaacga
     3721 gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc
     3781 gttttatgac acgctgacgc agtacgttga gatgggtaat agcacctttg cttgccctgg
     3841 acatcaacat ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt
     3901 tggcgagaac atctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct
     3961 gattcatgaa ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga
     4021 taaaacctat tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct
     4081 gttaacgcgt ggcgatttgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg
     4141 cgcgttgatt caggcggggg cgacgccggt ctatctggaa gcttcacgta acccgtttgg
     4201 tttcattggc ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg
     4261 cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt cgcctggcga ttattcagct
     4321 gggaacctat gacggcactg tctataacgc ccgtcaggtg atcgataccg ttgggcatct
     4381 gtgtgattac attctgtttg attccgcgtg ggtcggttac gaacagttta tcccgatgat
     4441 ggcggatagc tcgccgctgc tgttagaact taacgaaaac gatccgggga tctttgtgac
     4501 ccagtcggtg cacaaacagc aggcgggatt ctcacagacg tcgcagatcc ataaaaaaga
     4561 taaccatatc cgtggacagg cgcgtttttg cccgcataag cggttgaata atgcctttat
     4621 gctccatgct tctaccagcc cgttctatcc gctgttcgcc gcgctggacg ttaacgccaa
     4681 aattcatgaa ggggagagtg ggcgtcggct gtgggcggag tgcgttgcgt tggggattga
     4741 agcccggaag gcgattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt
     4801 tgatggcaaa ttgtggcagg attatccgac gtcagtgtta gccagcgacc gccgtttttt
     4861 cagttttgag ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt
     4921 tgttgatccg tgcaagctgt tactcaccac gccgggtatc gatgccgaaa ccggcgaata
     4981 tagcgatttt ggcgttccag cgacgattct ggcgcactac ctgcgtgaga acggcattgt
     5041 gccggagaag tgcgatctca actccattct gttcttatta actccggcgg aaagccacga
     5101 gaagctggca caactggtgg cgatgctggc gcagtttgaa cagcatattg aggatgactc
     5161 gccgctggct gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta
     5221 caccctgcgt cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga
     5281 cctacaaaaa gcgatgttcc gccagcagag tttcccgtca gtggtgatga atccccagga
     5341 tgcgcatagc gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg
     5401 gcgaattgcg gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg
     5461 ggaagtctgg ggtggggcgg ttcaacgtta tttccttgcg ctggaagaag gcgtgaattt
     5521 gttgccggga ttttcgccgg agttgcaagg tgtctatagc gaaaccgatg cgaatggcat
     5581 gaaacggttg tacggttatg tgttgaagta agaataaaaa aacgggtcac cttctggcga
     5641 cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg acacgaacgt
     5701 gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa cctgcgaaaa
     5761 tcagccatac ggtctgccag tcggtaatgc cgttttgggt gtacatctca accactttac
     5821 cgctcacgat gccgccgagg atacagccga agccgttggt catcatcagg aacatcccct
     5881 gcgcgctggc gcgaattgcc ggactaactt ctttttcgac aaacaccgaa ccagagatgt
     5941 tgaagaagtc gaatgcgcaa ccgtaaacga tcatcgacag taccagcagt acagtaccga
     6001 acggagtcgg gtcgccgtaa gcaaacagag caaaacgcag gatccacgct acaatactga
     6061 tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca
     6121 gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcacg atgaagctgc
     6181 tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac atgttggtaa
     6241 tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata cgcttgtttt
     6301 taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc gcttgctgtt
     6361 tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaacggcg gaaagagctg
     6421 cgccaatata cagctgcatg tggcttaatt cgaagccgga caggctcacc acccacattg
     6481 ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta acgatatcca
     6541 tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc gttggcatat
     6601 aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtagtg acctctgccg
     6661 ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atagacccat ttcgcactta
     6721 accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg atacccagtg
     6781 agctataaac tgcgccgata gaagcaccgt caaacttcag ggtaacaaac atataggagc
     6841 cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg attttcagct
     6901 gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc
     6961 acatttgacc gtgcgagatg atgcgattct atcaacttcg cggactggtt ttttgttacc
     7021 tgtggcaaat aattgcaaac attcccaatt tgcaaaacga aattgtgatg tggataacat
     7081 ttttacccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg taagattttt
     7141 gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg cgggtcgtca
     7201 gcgtctgata aacatcgccc ggcgtcatgg tgttaataat attggcagcc tgaatcttat
     7261 cattcgaaaa gactcgtagc acgctgcctg cgccgccgtt ataggcggtg atgacggcat
     7321 aacgccgcga tgttgggtta tcaattccgc cgagataaac gttgttcagc atcgccagat
     7381 acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc
     7441 cggatttccc ctgcgagcgg aacacatctt tcccggcggt atgttgtacc acctgcatta
     7501 atcccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca gtctgcataa
     7561 ttgccagaat cagcgactca tcaacgccat atttgcgtga cgcctggcgg accataccga
     7621 gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg acgctgtaga
     7681 tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa tccgcgaagt
     7741 tgctcgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa
     7801 ggaaaggttc tttcgaaatc gtaatatcat caacgtcgga atagagatcg accgaactcg
     7861 gatcgtcccc catcagcaac gttttgatta ttgcccggcg caaatgcgcg gcaggttctg
     7921 tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct
     7981 gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc tctttgaagc
     8041 cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa ccgttggtat
     8101 ctttgaccca ggcttcgtta taggtatcgc cttttttggt cgtcgaacag gagatgagca
     8161 acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt
     8221 gtttttatgc aagttgccgg aggcgtgctc cggcactgtt tttatttttt atcttccggc
     8281 gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat ctcctgctcc
     8341 agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat cagcatggtt
     8401 tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat gcgttttccc
     8461 agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag gaaagtacaa
     8521 aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac cggcgcgcca
     8581 gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga cggcggttgc
     8641 gctaagttat accagagcgc attgtcttca tccatgcagc cggtgaatga cgacacggga
     8701 agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa cgcggtcagc
     8761 tgcgtcaggt tatcggcaga aatctgtcgt tgcgccagcc actgccgcaa actttcttca
     8821 tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc
     8881 agcaatactt catcttcgtg ctgtaacagc aaaaagtagc cggtgcgctc tggcagcgtc
     8941 tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggcggcggc aatacatccg
     9001 ttttgtagcg gacagagcga acatttcggc ttagagcgcg tacaaatcat cgcgcccaaa
     9061 tccatcatcg cctggttaaa ccgttccacg ccaaccgcgg gcgtcacctg ctcgctcaaa
     9121 ctccatagtt tattctcgac ctcttttttc ccgggccagc cgcttacagc atagcagcgc
     9181 gccagcacgc gtttgacgtt accgtcgaga atcggaaagt gcttacccag agaaagcgag
     9241 agaatcgcgc ctgcggtgga acgcccgacg cccggcagta ctgcaacttc ctcaaaggtt
     9301 tccgggaatt taccgccgtg taaggccgcc acttgttgtg ccgctttatg cagattgcgc
     9361 gctcgggcgt aatagccaag cccggtccac aagtggagaa cttcgtcgag cggcgcattg
     9421 gcgagatcgg tcaccgttgg gaagcgcacc ataaagcgtt caaaataggg gataacggtc
     9481 gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg cgtcttgtca
     9541 atttgccagg gcagcgtttt ccgcccgtat ttatcgtacc agtccagaac ctgggctgaa
     9601 aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat tgcagcacag
     9661 cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc ttgcatccgg
     9721 caactaactt tggataatgc ccgttttcag aacactttca caagcgacta aatctttatg
     9781 aaaaacgacg tcatctcacc ggaatttgat gaaaacggcc gcccgctgcg ccgtatccgt
     9841 agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct ggaaaactac
     9901 tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccctgc gctttttggc
     9961 cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gcgcgtcgtt ggtggcaatg
    10021 gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc gggcgttggt
    10081 gcgtgcctgt cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat gtgtcacgat
    10141 gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt gcagctcttt
    10201 ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca ggtgccgttt
    10261 gccgagctgg taaaaagcaa actgcagctg gggggcatat tccatatggc gaccgactgg
    10321 gaaccttatg cggaacatat gcttgaagtg atgtcttcta ttgacggtta taaaaacctg
    10381 tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa atttgaacaa
    10441 cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt gaaataatgg
    10501 caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc caggaattag
    10561 gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt gataaaaccg
    10621 ttgatgactt tattaacgag gttatcgaac cgaacaaact ggcctttgac ggcagcggtt
    10681 atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc gaagaacatc
    10741 aggcgattgt gcgtaagtgg ctggaagaac gcaaaatgga agaggtacgc accagcgaac
    10801 ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca aaactgcctg
    10861 atgtgctacg cttatcaggc ctacaaagat gcacgatcga gtaggccgga taaggcgttt
    10921 acgccgcatc cggcatggaa aacgcgcact ttgttatcaa tctggggcca gcaaatgctg
    10981 gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt
    11041 cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg
    11101 tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc
    11161 cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc
    11221 aggcgaagga ttatcaggct gaactccgta gcaccctgcc gtggattgat gaaggcgcga
    11281 aaagccgcgt cgaaaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg
    11341 aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa gagcagatga
    11401 accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc attgatcagg
    11461 ttcgcgccga aggccagcaa ttagtgaatc aggcaatggg cggaatttta caggacagca
    11521 ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta cagaacgtgc
    11581 tgggaagcct gggcggtctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag
    11641 atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca
    11701 aagccctggt cgggaattta aaataatcct ctattttaag acggcataat acttttttat
    11761 gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc
    11821 acgttgtaaa ttgtttaacg tcaaatttcc catgcaaagc taagggataa tgcgtagcgt
    11881 tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt
    11941 atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc aaccggcggg
    12001 accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc
    12061 gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt taaaggcgag
    12121 caggtagtga atattggctc ccaggacatg aacgatgatg tctggctgac actggcgaaa
    12181 aaaattaaca ccgactgcga taaaactgac ggcttcgtca ttacccacgg taccgacacg
    12241 atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg
    12301 gtcggtgcaa tgcgcccgtc cacgtctatg agcgcagacg gtccattcaa cctgtataac
    12361 gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtactggt agtgatgaac
    12421 gacaccgtac ttgatggccg tgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc
    12481 aagtctgtta actacggtcc tctgggttac attcacaacg gtaagattga ctaccaacgt
    12541 accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct gaatgaactg
    12601 ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc taaagcactg
    12661 gtagatgcgg gctatgatgg catcgttagc gctggcgtgg gtaacggcaa cctgtataaa
    12721 accgtatttg acacccttgc aaccgctgcg aaaaacggca ctgcagtagt gcgttcttcc
    12781 cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa atacggcttc
    12841 gtcgcctctg gcacgttgaa cccgcaaaaa gcgcgcgttc tgctgcaact ggctctgacg
    12901 caaactaaag atccgcagca gatccagcag atcttcaatc agtactaatc acttcgcccc
    12961 ggtatcgtgc cggggcttat tcacttcaga ctcacgtcca ttgccaattt ttattactct
    13021 aatgataatc accggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga
    13081 ttgttggtac ggcgctgctt atgttgatga ctggtaatgt ccgggcagat ggtgaaccgc
    13141 caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg
    13201 atgtcatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg tcagcggaag
    13261 gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgagc
    13321 tgctcgaaca gacctggacg aaagataaac cggtgaaatt ctcggcgatg ttaaccagta
    13381 aaggaacgcc cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg
    13441 atcgtgggcg ggtggttgac gatattaaaa cgaataataa atatctgatc gtgaatagcg
    13501 aagatttcaa ttatcgcttt agtcagcttg agtctgcgtt gaataaccag aataactcga
    13561 ttcctgcact gaaaaaggat gtaaaagcgc ttgataagca aatggtggcg gcgcagaaag
    13621 cagcggatgc atattgggga aaagatgcaa acggtaagca aaggacccgc gaagacgcct
    13681 ttaaaaaaat tcaccaacag cgtgatgatt ttaataaaca gaacgatagc gaggcgttcg
    13741 cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg
    13801 aagagtgtta tgaagtgcca attcagcaga aacgagattt tgatatcaac gaacaacggc
    13861 gacagacttt tctacaatca caaaaactca gccgtaaatt gcaggatgac tggataacgc
    13921 tcgagaaagg gcaatatcca ttaacgatga aggtttcaga aataaacagt aaaaaagtcg
    13981 cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa aaagataccg
    14041 aacaactgcg gcgcaatggg gtgattaagt aatttttgcg atgacgcaag agttgtagcc
    14101 ggatgttgcg tacacgccgc atccggcaat acaaatttac tcagccagaa aaagctccag
    14161 cagcgaattt aaaaacaact tcccatgttc cgttatctgc cagtaatccg cacattctgt
    14221 gagataaccc tgagcgatag cctcgtctaa ctgagaacga ataactgctt cagaaaggcc
    14281 tgtatactgg ctaaactcca cgcgcggcgc aggttccagc agacggaagc gattcataaa
    14341 gaactcaaat ggtttatctg cggcttcgac atctcgctgg ctttccagat accgcccctg
    14401 cataaaacca cgtggatgac gcgttttggt ggtacgcaga atgcgcccat ccgggaaggt
    14461 cactttgcca tgcgcgccgc agccaatacc gatgtagtca ccaaagcgcc agtagttgag
    14521 attgtgctgg cactgataac cgggtttggc gtaagcggaa gtttcatatt gctgataacc
    14581 cgctgcggtt aataactgat gcccctgttc gaaaatatcc cacagcgcgt catcgtccgg
    14641 cagtacagga gggcgcgagc caaacagcgt attaggttcg atggtcagtt gataccagga
    14701 aagatgcggc ggattcagtt caatggcctg gcgcagatcg ccaagcgcct cttccagtga
    14761 ttgatccggc agcccatgca tcaaatcaag gttaaaacta cgcagcccca gcccgcttgc
    14821 cagattcgca gctcgtttcg cttcttgcgg gccatgaatg cgcccaagtc gtttcagctt
    14881 ttcttcgcta aaactctgta cgccaatgga gatgcggttc acaccagcac gctgataatc
    14941 aacaaagcga tcggcttcaa ccgtaccagg attcgcttcc atcgtaattt ctgcatccgc
    15001 tgccagcggc aaacgcgcac gtacgccgtc cagcagcgtt tgcatcgccg ggccggaaag
    15061 caggctcggc gtaccaccgc caataaagat tgtctttact tcacggccct gagcgtaagc
    15121 cacatcgttg ttcagatcgt taagcagatg ctgaacataa tcgtcgtgcg gcacttctcc
    15181 tttcaacgcg tgagagttga aatcgcagta cgggcatttc tgcacgcacc acgggatgtg
    15241 aatgtagaga ctcagcggcg gtaatttaac cattacgtaa agcgtccagc agcagtttca
    15301 acgcctgacc acggtgggaa atggcgctct tttcttcgcg ggtcagttcg gcagcagttt
    15361 tcccttcgga aggtacgaag aagattggat cataaccaaa gccaccagtg cccgctggtt
    15421 cacgagtaat cacgcccggc cagctaccgt ggcacaccag cggagtggga tcttccgcgt
    15481 gacgcagata taccagcacg cagtggaaac gcgcctgacg ttggtcgtcc ggtacgtctt
    15541 tcattgtttc cagcagtttt tgcagattct tttgatcggt cgcgtcttca ccggaataac
    15601 gcgcggagta aatacccggc gcgccaccca gcgcatctac cgccagaccg gagtcgtcgg
    15661 caattgccgg taaaccggtc acttttgccg catggcgcgc tttcagaatc gcgttttcga
    15721 taaaggtcag gccggtttct tcagcggaat ctacgccgag gtctgtttgg gcaacgatat
    15781 caagaccgaa gtcgctaagc agcgacgcca gctcacgcac tttgccggca ttgccggttg
    15841 cgaggacaac tttttgcata ggatacctaa ttaattaacg ccgcgatttc tggcgggatt
    15901 tgttgcggat taatgatttt aatttgtttg tggcggccaa gttcgccttt ttcaatcacc
    15961 acctggcttt tggcaacccg gaattgctta ccgagaaact tcaccagatg gctgttggcc
    16021 tggccgtcaa ccggcggcgc ggtaatggcg acttttacct cgtcgccatg taaaccgaca
    16081 atagaatcac ggctggcttt cggctgaata tagagccgta aaaccagacc gtcatcatta
    16141 actgttacgg cactcataac gccatccaca gccccggcag cagcatattt ccggtcgcct
    16201 gcaagacttc tgcgacaccc atattgatga catacagcag cagaacgagg atcatcggcg
    16261 agaaatcaat cccacccatt gccggtagca agcggcgaat cgggcgcagc agcggatcgg
    16321 ccagctgaat cagcacgtat tcaatcgggc tacgaccctg gcttacccag ctcataatcg
    16381 ccatcaccag caggacccag aatatcagca ggccgatggt tttcagcaga atcaataaac
    16441 cggcaatcca aatgattggc aggaaggtca ccactttaaa cagcacgatg gctttaacga
    16501 aactgagaat ataggcaacc agcagcgagg cgctatcaat ggggcccatt gccggaataa
    16561 cgcggcgcag tggcccgata atcggctgcg ttaccttcac tacaaactgt gagaaggggt
    16621 tgtaaaaatc acaatgagcc cactgcatcc agatgcgtaa taacagcacc atggtataca
    16681 gctcaatgac cgttgaaagc aggaaagtca acgtattcat ggcgttcctt taattcctta
    16741 ttttttagag taatcacgcg caccaaaaat tgcagtgccg atacgaacca tcgtgctacc
    16801 tgccgcaatg gcggcttcca tatcgtccga cattcccaga gagagcgtgt cgatatgcgg
    16861 gtagcgcgtt ttcagtccgg caaatgctac agccatttgg cgtgcaactt caaactgcct
    16921 tacatattct gactcaggcg cagggattgc catcagcccg cgcagacgta aacgcggtag
    16981 ttcagcgacc gcagctgcca gctcgtccag ttcagccagt tgaatcccgg acttactgtt
    17041 ttcatcacta atgttaattt gaatcagtac gttaagaggg ggaagttctg ccgggcgctg
    17101 gtcgttcaga cgggtagcga tgcgcaaacg gtcgatggta tgacaccagt cgaaatgctc
    17161 tgccaccagg cggcttttat tagactgcaa cgggccaata aaatgccatt ctaatcctgt
    17221 tacgcccagt tcctgaaagt agcgaatttt atctacccct tcctgaacgt agttttcacc
    17281 aaattgacgc tgcccggcat caatggcttc tgcgatggcg ctcgcaggtt ttgttttact
    17341 gactgcaagc agcgtaattt cttctggaga acggccgcaa cgcgttgcag ccgctgagat
    17401 tttgtcccgg acctgtgcca ggttatgcgc aatatcgttc attttccgag gatcttagta
    17461 tgaatatgga agaaattgtg gcccttagtg taaagcataa cgtctcggat ctacacctgt
    17521 gcagcgcctg gcccgcacga tggcgtattc gcgggagaat ggaagctgcg ccgtttgacg
    17581 cgccggacgt cgaagagcta ctgcgggagt ggctggatga cgatcagcgg gcaatattgc
    17641 tggagaatgg tcagctggat tttgctgtgt cgctggcgga aaaccagcga ttgcgcggca
    17701 gtgcgttcgc acaacggcaa ggtatttctc tggcgttacg gctgttacct tcgcactgcc
    17761 cgcagctcga acagcttggc gcaccaccgg tattgccgga attactcaag agcgagaatg
    17821 gcctgattct ggtgacgggg gcgacgggga gcggcaaatc taccacgctg gcggcgatgg
    17881 ttggctatct caatcaacat gccgatgcgc atattctgac gctggaagat cctgtggaat
    17941 atctctatac cagtcagcga tgtttgatcc agcagcggga gattggtttg cactgtatga
    18001 ctttcgcatc gggattgcgg gctgcattgc gggaagatcc tgatgtgatt ttgctcggag
    18061 agctgcgtga tagcgagaca atccgtctgg cgctgacggc ggcagaaacc gggcatctgg
    18121 tgctggcgac attacacacg cgcggcgcag cgcaggcagt tgagcgactg gtggattcat
    18181 ttcctgcgca ggaaaaagat cccgtgcgta atcaactggc aggtagttta cgggcagtgt
    18241 tgtcacaaaa actggaagtg gataaacagg aaggacgcgt ggcgctgttt gaattactga
    18301 ttaacacacc cgcggtgggg aatttgattc gtgaagggaa aacccaccag ttaccgcatg
    18361 ttattcaaac cgggcagcag gtggggatga taacgtttca gcagagttat cagcagcggg
    18421 tgaaagaagg gcgcttgtga cgttgctgtg tctgatacga attgtaaata tgcccgatgc
    18481 gcaagcttat cgggcctaca agggaagcgt aactttgaat tcgcactcac tgtaggttgg
    18541 ataaggcgtt tacgccgcat ccaacatgaa acaacgagca cattgtcaga aatctgtgcg
    18601 ctagtaaatc gccttaatat ccctgctcga aatagctttc gagaataata accgcagagg
    18661 cagagtcgac tttgcttttg ttgagcgccc gatagccgcc ctgttcaaac agaccggaac
    18721 gtgcctccac ggtgctaaga cgctcgtcat ggagctttac ttcaacaccg aaacgaccat
    18781 gaatacggtt ggcaaattta cgcgctctgg cggttaacgg ctgttcggtg ccgtccatat
    18841 tcagcggcaa accgacgatg atttcgtccg gctgccactc tttcagtaaa cgctcgataa
    18901 tgttccagtc cggcgtaccg tcctgcgctt taattgcagg caaagggcga gcggtgccgg
    18961 taatgcgttg gccgaccgct acgccaatgc ttttggtgcc gaagtcgaag gcgagtaagg
    19021 ttccactcat caggcgtgtc ctgccacacc aggcatggtg agaatatcca caccaatcag
    19081 ttttgccgcc tcgcgccagc ggtccgcaat cggcgttttg aacagaatat tcagatctgc
    19141 cggggccgtt agccacgcgt tatcgagaat ttcttgttcc agttgacctt tctcccagga
    19201 ggcataaccc agagccacca atacgtcaga cggttgttta tcggtgccga gcgtttccag
    19261 cacatcgcgg gaagtggtca tcactgtgtt gtctgaaatg cgaatgctgg aagcaaaatt
    19321 ggagggcgga gtatgcaaaa taaacccgcg atcttcagcc agcggaccgc cgagcataac
    19381 cggtttatcc agacggattg attcatcacg cggctccggc gtgatcttca gtttttccag
    19441 aatcccttca attttgagat tttccagcgg cttgttgacg atgatcccca ttgcaccatt
    19501 ggtattatgt tcgcaaatgt agaccacgga acgacggaaa atcggatcct ggagagcagg
    19561 catggcaata agaaagtgat gctgtaaatt cattgtcaga ggttctgttt cctggttcaa
    19621 aaaagcaaca gcgcccagta tgcgtggaaa gaagggcgct gtcactcaga gtctcaacga
    19681 gatccttctc gctaaggtgg gttactgctg ctgtaaacgt gcttcgatgg catccattaa
    19741 cattccggta atcgacaccg gaaactctgc ttcaatctca cgaatacagg ttgggctggt
    19801 gacgttaatt tcagtcagac ggtcgccgat gatatccaga ccaacaaaaa tcagcccttt
    19861 ttctttcagc gtcggcccaa tctgacgggc gattttccag tcactttccg tcagcggacg
    19921 aggttcaccg cgaccaccgg cagccagatt gccacgggtt tcgcccccct gcggaatacg
    19981 cgccaggcaa tacggcaccg gctcgccatc cactaccagc acgcgtttgt cgccatcttt
    20041 aatggctggc aggtaatttt gcgccatgca gtagcgagtg ccatgctcag tcagggtttc
    20101 ggcaatcacg ccgaggtttg gatcaccttc tttcacgcgg aaaatcgacg cgccgcccat
    20161 accgtccagc ggcttaagaa tgatgtcgct gtgtttttcc cagaaagctt ttagctgcgc
    20221 tttattgcgt gtgaccagcg tttctggtgt taagtcagag aaccaggcag tgaacagttt
    20281 ttcgttacaa tcgcgcaggc tctgcggctt gttaacgatc agcgtccctt tatcttcggc
    20341 acgttccaga atataggtcg cgtagataaa ctcggtatca aacggcgggt ctttacgcat
    20401 caggatcaca tcgagatcgg ccagcggcag atcctgctca cccgtgaact cgtaccattt
    20461 atcgtagttc tgttcgacgc tcaaagtatg ggtacgagcg cgagcctcac cattgatcaa
    20521 atagagatcg gccatctcca tatagtgaag ttcgtaacca cgacgctgtg cttccagcaa
    20581 catagcgaaa ctggaatctt tcttgatgtt gatgtttgcg atggggtcca tcacgatgcc
    20641 gagcttgatc attattcttc tccgttagcc caaatcgcca aaacgcactt gtagcgcggt
    20701 aatggcggtg agcgcagttg tctcagtacg caaaacgcga ggtcccaaca ggatatcagt
    20761 aaattgatag cgggcagtca tggcaatttc atctgccgat aaaccgcctt ccgggccaat
    20821 cagcaggcgg acgcgttcga ccggtaacgg caacgtattg atgctgttac tggcgcgcgg
    20881 gtgaagattc agtttcagtc cttcatcctg ctctgcacac caggcttcca gatccatcgc
    20941 tggacggatt tccggcaccc ggttacggcc acactgctca caggcagcaa ttgcaatctt
    21001 ctgccactgc tggagcttct tgttcagacg ttcactatcc agtttaacgc cgcagcgctc
    21061 agaaaaaagt ggcgtaatga ggcttacacc gagttcgatc gatttctgga tagtaaattc
    21121 cattttttca ccacgcgaca tcacctgacc gaggtgaata tgcagcggag attcgcgatc
    21181 gtcgatctgg ccttccagca ccttcacttc cacgcttttt ttgctggcgc tggtaatttc
    21241 ggcgtcaaag acctggttgc tgccgtcaaa caattgcagc gcctgtcccg gccccatacg
    21301 cagtacgcgc ccgatatggt tggcggcatc ttcgcaaagc gcgatgtgag aatggctggt
    21361 cagtggttca ggatgataaa tgcgggggat acgcatagtt aaaaatccgc gtcgtctccc
    21421 cacgcggttg tacgcgtggg gtaggggtta acaaaaagaa tcccgctagt gtaggttagc
    21481 tctttcgcgc ctggcaagcg cgttgcacat acgggttatg attgccctgc accttcgcga
    21541 tgcgttcatc acgctcgcac tcccagtcgg taactggata catcttgtcc catgcgttga
    21601 acagctgcgt ttgctgacga gagagtgtca ggttgtattg gtcacgcata tagaagtagg
    21661 tgcgcgcaat ggcaccgcgt gcacgcgctg gtggctcggc aactttttct ttgaaatcga
    21721 ccttcatggc gcattgaccg tactggcctt caccgccatt ccactggctg tacataaagt
    21781 tgccgcgatc gccattcacc tcaccgactg acggctgcag gttatgcata tcgctttcca
    21841 tcttgcgata gaccggatct ttggcgcaat ttttacgtcc accgtcctgc cagcactggc
    21901 gctggtgacc gaattgccag gcaggaacga cgtgttccca ctctacgcgg ctggcacggt
    21961 tttcattttt tcgcacctga tagccgcacg attgcagatc aacaacgcct tttttgccct
    22021 gccagttaat tttacatccg caataaaacg taccgggcgc gtcagcgtgg acttttaccg
    22081 ccgcggcttt cgcttgagaa aaactattga taccttcggc caatgccggg ccggaaaatg
    22141 ctgcgctcag taccaccgca gcaatagaca aataacggta catcacttac tccgtgttaa
    22201 aacgagccag caacgtagcg aaagttgttt tggtatgcaa tcagccagat caggaaagtt
    22261 cctgatagtt caggtggtta tttcgcaacc agctgttcgc cgcagtgaac acagcgatag
    22321 acggcttcgc cacgaactac gcgattatga cggcgtacgg taagctgatg ctcctggcac
    22381 ttgcagcggt aggggaaggt gttgcggcgc acggattgca gttcgaactg atgcgtacgg
    22441 cgggcgggaa cgcccagcac gctttccatc atccacttcc actctttgcc atgtggcgct
    22501 acgcggccga aatgtttcca taccagcaaa tgtgccagtt cgtgcggcac cacttcttca
    22561 ataaaggctt cactgttttc caacagcaaa acgggattga gacgaatttc atagctttcc
    22621 agccaggctg ttccggcaga ggttccgcgc tgggtgtaag agagttttgg ctccgggtag
    22681 ttacgcccta gcttcaggtt ggcctgggcg agtttttccc gcaggcgacg cataacggcc
    22741 tgttggatgg cgatagggag acgggatgtt ttcatagcgg cagaggatag agcgaagaaa
    22801 agatgactgc aagaggtggc ttcctccatg atgggaggaa gcctgaggag attaatcgtg
    22861 agcgccgatt tcgcgcagtt tacgaccttt catcagatta cgttcaatat gttccagcga
    22921 aacgtgtttg gtttccggta ccagccacaa tgtcagcagg ataaacagta cgttcagagc
    22981 agcatacacc cagaaggtgt tggcgttacc cagcgtgttg agcatggtca ggaacgttgc
    23041 gccaacgatc atgttggcaa tccagttggt ggcagtggag ctggtgatgc caaaatcgcg
    23101 gcctttcagc ggctgaattt cggagcacag tacccaaatc agcggaccgg cactcatggc
    23161 aaaaccgaca ataaacatca gcagcatggc gatggcgaaa tactgcgccg acggagagtg
    23221 gataccgata tgcatcattg taccgagtac gcccatacca gcagccatca ccaggaagcc
    23281 cagcgttagc gttggtttac gtccccagcg gtcaacaagg ccgattgcga taaaggtggc
    23341 aagtacgttg gtcaggccga caatcactgt cccccacatt tgctcggtgg tgttggtata
    23401 acccgccagt tcgaagattt tcggcgcgta atacatgatg acgttcatcc cggtgaattg
    23461 ctgcattacc tgcaatagta cgccaaggaa caccgcgcgg cggaagttgc tattctcttt
    23521 aaacagcgcc cagccactct gtttaacctg caaactttca cggatttcat ccagttcgcg
    23581 tttcgcttcc gcgctggtgt cacgcaggcg tagcagcacg cgttcggcat caacaaaacg
    23641 gcgtttggcg gcaaaccaac gtgggctgtc tggcaggaag aagacaccaa tcagcagcaa
    23701 aattgccggg atgataatca cacccagcat ccagcgccat gcaccggtgt agctgaaggc
    23761 ggtatcagaa agataagcgc cgaggatccc gatagtgatc atcaactgat acatcgagat
    23821 catactgccg cgaatttttt ccggcgcaat ttcagagagg tacagcggtg cggtataaga
    23881 ggccacaccc actgccagcc ccagcaaaac gcgggaaaga atcagtactt caacgtttgg
    23941 cgcagcagca gagaacagtg aaccggcaac aaacaagatt gcgccgatca tcaggctctt
    24001 tttgcgcccg agtttaaagg agagccagcc gctgcccacc gcaccgactg ccgcaccgaa
    24061 catcatggag cttacgaccc attcttgcgt gtgcgaagta atctggaatt catctgcaat
    24121 aaacggcagt gcgccagcaa ttacaccgat atccaggcca aagagtaatc ccgccagagc
    24181 ggcaaggaag cagacgaaaa acgtcattgc cttgtttgac cgcccctgtt ttttagcgtc
    24241 aggcatgatg ccctccaata tggttatttt ttattgtgaa ttaagatagg tgagtacgac
    24301 gtaaaaagat gtgaagcaaa tcacatcagt gtaatcgctt acactaacat ttatcttagt
    24361 tatgatttaa gtgcatgaaa ataattgagt tatatcatca tagattaaag gttgttatct
    24421 gcaattaaga ctttactgaa aaaaaatgta acaactgtga aggctgaata ataatatgaa
    24481 aatattacaa atatttggct ggcgtggttg gtgtaatcgg tttcagtagt gatgataatt
    24541 tgcggtaaaa cgtgttgagg aagaatgatg ttattgtgcg ggcggttttt ttgccggatg
    24601 cggcgtgaat gcctgtccac cgcggtgagg taaaagaaaa aggccagcgc gaggctggcc
    24661 tttgaaagca ggtgaagaaa gattacttca gaccggcagc atcacgcagc agctgcgctt
    24721 tgtcggtttt ttcccacggg aaatgttcac gaccaaagtg accgtatgct gcggtttctt
    24781 tgtagatcgg gtgcagcaga tccagcatct gaatcagacc gtatgggcgc aggtcgaaga
    24841 attcacgtac cagcagagtc agttgttcag aaggcacttt ctcagtaccg aaagtttcta
    24901 ccatgatgga agtcggttca gccacgccga ttgcgtagga aacctgaatt tcacaacgat
    24961 cggccaggcc agcagcaaca atgtttttcg cgacataacg tgctgcgtag gctgcggaac
    25021 ggtccacttt tgatggatct ttaccggaga atgcaccgcc accatgacgc gccatgccgc
    25081 cgtaggtatc aacgatgatt ttacgaccag tcagaccgca gtcacccatc gggccaccga
    25141 taacgaaacg accggtcggg ttgatgaaga atttggtggc agaagtcagc cattcagcgg
    25201 gcagaatcgg cttgatgatc tcttccatta ccgcttcttg cagcgatttc tggtcgatct
    25261 cttcagagtg ctgagtggaa agcacgacag catcaatgcc aacgattttg ccgtcgtcat
    25321 actggaaagt cacctggctt ttcgcgtccg ggcgcagcca cggcagagtg ccgtttttac
    25381 gcacttcagc ctgacgctgt accagacggt gtgcataagt gataggtgct ggcatcagca
    25441 cgtcggtttc attagttgcg tagccaaaca tcagaccctg gtcgcccgcg ccctgttcca
    25501 gcggatcggc acggtcaacg ccctggttga tgtcaggaga ctgtttaccg atggcgctca
    25561 gaaccgcaca ggagttagcg tcaaagccca tgtcggaatg cacatagcca atttcgcgaa
    25621 cggtattacg ggtgatctct tcgatgtcta cccaggcgct ggtggtgatt tcgccgccaa
    25681 ctaaaaccat gccggttttt acgtaggttt cgcaagcaac gcgtgctttc ggatcctgtt
    25741 cgaggatcgc gtctaaaacg gcatcagaga tttggtcagc aattttgtca ggatgccctt
    25801 cagagacgga ctcggacgta aaaaggtgtt ttgccatatt taatatcacc taaagagaat
    25861 ttggttagct caaactgttg tgtggatttt atgtggtagc ggatcctacc acgactctgc
    25921 aggttaaaaa cactggcagt ctgagtgtta atcggtatgg atggattaac atctggatgg
    25981 ctattttagg tcagttcttc acccaatttc cacttttttt tgaatcgtgt ctcattctgt
    26041 taaaaacgtg gctggaaatt tttcctgaca atgctggcat tctgcgtatt tatcttttgc
    26101 aattttctgc cattgtgggg tataaaacgc ggcgcgcggc ttaaataaaa agcacacgac
    26161 gtttctttcg tgttgccact tccagccggg ttgaaaacag agttttggct tgtgggttcg
    26221 tcttaacagg cggccgtgga ggtgatacga aataatgaac cgttgtctgc tgcttaacct
    26281 gtctcaccgt tctggtgaag attcgttccc cgcactctgc atctctgctt tgcatacctg
    26341 ccgatgttat acccatctcg gcgcttctca ggattcaaga gctggttaca gctactgagg
    26401 actgaacaag ggcgctcttg taaaaacaag agttttctcg tggtttcgcc gaactgtcac
    26461 acttacgttc ggttatgtgc ttaataatgt tatgaaaaag aaaccggttg cgcagttgga
    26521 gcgtcagcat tcactgctgg aaaatccatg tgcttatggg ttgttatcgc agttccaggc
    26581 tgcgatagtc gttaactgtt ttacacttaa taaaataatt tgaggttcgc tatgtctgac
    26641 gacatgtcta tgggtttgcc ttcgtcagcg ggcgaacacg gtgtactacg ctccatgcag
    26701 gaggttgcaa tgagctccca ggaagccagc aagatgctgc gtacttacaa tattgcctgg
    26761 tggggcaata actactatga cgttaacgag ctgggccaca tcagcgtgtg cccggacccg
    26821 gacgtcccgg aagctcgcgt cgatctcgcg cagttagtga aaactcgcga agcacagggt
    26881 cagcgtctgc ctgcactgtt ctgtttccca cagatcctgc agcaccgttt gcgttccatt
    26941 aacgccgcgt tcaaacgagc gcgggaatcc tacggctata acggcgatta cttcctcgtt
    27001 tatccgatca aagttaacca gcaccgccgc gtgattgagt ccctgattca ttcgggcgaa
    27061 ccgctgggcc tggaagccgg ttccaaagct gagttgatgg cggtgctggc acatgctggc
    27121 atgacccgta gcgtcatcgt ctgcaacggt tataaggacc gcgaatatat ccgcctggca
    27181 ttaattggcg agaagatggg ccacaaggtc tatctggtca ttgagaagat gtcagaaatt
    27241 gccattgtgc tggatgaagc agaacgtctg aatgtcgttc ctcgtctggg cgtgcgtgca
    27301 cgtctggcgt cgcaaggctc gggtaaatgg cagtcctccg gcggggaaaa atcgaagttt
    27361 ggcctggcgg cgactcaagt actgcaactg gttgaaacgc tgcgtgaagc cgggcgtctc
    27421 gacagcctgc aactgctgca cttccacctc ggttcgcaga tggcgaatat tcgcgatatc
    27481 gcgacaggcg ttcgtgaatc cgcgcgtttc tatgtggaac tgcacaagct gggcgtcaat
    27541 attcagtgct tcgacgtcgg cggcggtctg ggcgtggact atgaaggcac ccgttctcag
    27601 tctgactgct ccgtaaacta cggcctcaat gaatacgcca acaacatcat ctgggcgatt
    27661 ggtgatgcgt gtgaagaaaa tggtctgcca catccgacgg taatcaccga atcgggtcgt
    27721 gcggtgactg cgcatcacac cgtgctggtg tcgaatatca tcggcgtgga acgtaacgaa
    27781 tacacggtgc cgaccgcgcc tgcagaagac gcaccgcgcg ccctgcaaag catgtgggaa
    27841 acctggcagg aaatgcacga accgggaact cgccgttctc tgcgtgaatg gttacacgac
    27901 agccagatgg atctgcacga cattcatatc ggctactctt ccggcacctt tagcctgcaa
    27961 gaacgcgcat gggctgagca gctttatttg agcatgtgcc atgaagtgca aaagcagctg
    28021 gatccgcaaa accgtgctca tcgtccgatt atcgacgagc tgcaggaacg tatggcggac
    28081 aaaatgtacg tcaacttctc gctgttccag tcgatgccgg atgcgtgggg gatcgaccag
    28141 ttgttcccgg ttctgccgct ggaagggctg gatcaagtgc cggaacgccg cgctgtgctg
    28201 ctggatatta cctgtgactc agacggtgct atcgaccact atattgatgg tgacggtatt
    28261 gccacgacaa tgcctatgcc ggagtacgat ccggagaatc cgccgatgct cggtttcttc
    28321 atggtcggcg catatcagga gatcctcggc aacatgcaca acctgttcgg tgataccgaa
    28381 gcggttgacg tgttcgtctt ccctgacggc agcgtagaag tagaactgtc tgacgaaggc
    28441 gataccgtgg cggatatgct gcaatatgta cagctcgatc ctaaaacgtt gttaacccag
    28501 ttccgtgatc aagtgaagaa aaccgatctt gatgctgaac tgcaacaaca gttccttgaa
    28561 gagttcgagg caggtttgta cggttatacc tatcttgaag atgaataatt aagattattc
    28621 cgctggcggg tcgatttatt tacgtaaat
//
LOCUS       DAZFRG010000035        28580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFRG010000035 DAZFRG010000000
VERSION     DAZFRG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.035"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHKUC_001199"
     CDS             complement(133..273)
                     /gene="yjjY"
                     /locus_tag="ACHKUC_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO6617485.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            369..1085
                     /gene="arcA"
                     /locus_tag="ACHKUC_001200"
     CDS             369..1085
                     /gene="arcA"
                     /locus_tag="ACHKUC_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO6617486.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1144..2496)
                     /gene="creD"
                     /locus_tag="ACHKUC_001201"
     CDS             complement(1144..2496)
                     /gene="creD"
                     /locus_tag="ACHKUC_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO6617487.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2554..3978)
                     /gene="creC"
                     /locus_tag="ACHKUC_001202"
     CDS             complement(2554..3978)
                     /gene="creC"
                     /locus_tag="ACHKUC_001202"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO6617488.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3978..4667)
                     /gene="creB"
                     /locus_tag="ACHKUC_001203"
     CDS             complement(3978..4667)
                     /gene="creB"
                     /locus_tag="ACHKUC_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO6617489.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4680..5153)
                     /gene="creA"
                     /locus_tag="ACHKUC_001204"
     CDS             complement(4680..5153)
                     /gene="creA"
                     /locus_tag="ACHKUC_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO6617490.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5364..6233
                     /gene="robA"
                     /locus_tag="ACHKUC_001205"
     CDS             5364..6233
                     /gene="robA"
                     /locus_tag="ACHKUC_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO6617491.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6230..6877)
                     /gene="gpmB"
                     /locus_tag="ACHKUC_001206"
     CDS             complement(6230..6877)
                     /gene="gpmB"
                     /locus_tag="ACHKUC_001206"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO6617492.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6929..7456
                     /gene="yjjX"
                     /locus_tag="ACHKUC_001207"
     CDS             6929..7456
                     /gene="yjjX"
                     /locus_tag="ACHKUC_001207"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO6617493.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEVLGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7529..7855)
                     /gene="trpR"
                     /locus_tag="ACHKUC_001208"
     CDS             complement(7529..7855)
                     /gene="trpR"
                     /locus_tag="ACHKUC_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO6617494.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(7945..9882)
                     /gene="sltY"
                     /locus_tag="ACHKUC_001209"
     CDS             complement(7945..9882)
                     /gene="sltY"
                     /locus_tag="ACHKUC_001209"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO6617495.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10089..11756
                     /gene="ettA"
                     /locus_tag="ACHKUC_001210"
     CDS             10089..11756
                     /gene="ettA"
                     /locus_tag="ACHKUC_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO6617496.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11812..12096)
                     /gene="nadS"
                     /locus_tag="ACHKUC_001211"
     CDS             complement(11812..12096)
                     /gene="nadS"
                     /locus_tag="ACHKUC_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6617497.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(12098..12430)
                     /locus_tag="ACHKUC_001212"
     CDS             complement(12098..12430)
                     /locus_tag="ACHKUC_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6617498.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPINAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(12522..13754)
                     /gene="nadR"
                     /locus_tag="ACHKUC_001213"
     CDS             complement(12522..13754)
                     /gene="nadR"
                     /locus_tag="ACHKUC_001213"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO6617499.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLIDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13775..15157)
                     /gene="radA"
                     /locus_tag="ACHKUC_001214"
     CDS             complement(13775..15157)
                     /gene="radA"
                     /locus_tag="ACHKUC_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO6617500.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSVDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(15206..16174)
                     /gene="serB"
                     /locus_tag="ACHKUC_001215"
     CDS             complement(15206..16174)
                     /gene="serB"
                     /locus_tag="ACHKUC_001215"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO6617501.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            16280..16924
                     /locus_tag="ACHKUC_001216"
     CDS             16280..16924
                     /locus_tag="ACHKUC_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO6617502.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16952..17968
                     /gene="lplA"
                     /locus_tag="ACHKUC_001217"
     CDS             16952..17968
                     /gene="lplA"
                     /locus_tag="ACHKUC_001217"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO6617503.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            18000..18263
                     /locus_tag="ACHKUC_001218"
     CDS             18000..18263
                     /locus_tag="ACHKUC_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6617504.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18424..19143)
                     /gene="deoD"
                     /locus_tag="ACHKUC_001219"
     CDS             complement(18424..19143)
                     /gene="deoD"
                     /locus_tag="ACHKUC_001219"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO6617505.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19200..20423)
                     /gene="deoB"
                     /locus_tag="ACHKUC_001220"
     CDS             complement(19200..20423)
                     /gene="deoB"
                     /locus_tag="ACHKUC_001220"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6617506.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20475..21797)
                     /gene="deoA"
                     /locus_tag="ACHKUC_001221"
     CDS             complement(20475..21797)
                     /gene="deoA"
                     /locus_tag="ACHKUC_001221"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO6617507.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21875..22654)
                     /gene="deoC"
                     /locus_tag="ACHKUC_001222"
     CDS             complement(21875..22654)
                     /gene="deoC"
                     /locus_tag="ACHKUC_001222"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO6617508.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22912..24462
                     /locus_tag="ACHKUC_001223"
     CDS             22912..24462
                     /locus_tag="ACHKUC_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO6617509.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IVARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24434..25297
                     /locus_tag="ACHKUC_001224"
     CDS             24434..25297
                     /locus_tag="ACHKUC_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO6617510.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(25514..26293)
                     /locus_tag="ACHKUC_001225"
     CDS             complement(25514..26293)
                     /locus_tag="ACHKUC_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6617511.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQQGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(26290..27363)
                     /locus_tag="ACHKUC_001226"
     CDS             complement(26290..27363)
                     /locus_tag="ACHKUC_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO6617512.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27485..27646)
                     /locus_tag="ACHKUC_001227"
     CDS             complement(27485..27646)
                     /locus_tag="ACHKUC_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO6617513.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27773..28378)
                     /gene="osmY"
                     /locus_tag="ACHKUC_001228"
     CDS             complement(27773..28378)
                     /gene="osmY"
                     /locus_tag="ACHKUC_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO6617514.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
BASE COUNT         6791 a         7839 c         7187 g         6763 t
ORIGIN      
        1 attattaaaa taatgtgacg aaagctagca tttagatacg atcatttcat caaactgtta
       61 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtcctgcc
      121 cctcttagcc tgttatattg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
      181 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
      241 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
      301 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
      361 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
      421 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
      481 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
      541 cgggtaaaaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
      601 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
      661 atgactacat caccaaaccg ttcaacccgc gtgaactgac tattcgtgca cgcaacctgc
      721 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
      781 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
      841 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
      901 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccgcacg
      961 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     1021 cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt gatctggaag
     1081 attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     1141 accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt
     1201 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
     1261 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     1321 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctattac gccagccttt
     1381 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     1441 tgccacagta aaaccggtat gttcagaaag cgccagcaag agcaaataaa acatcaccaa
     1501 tgacagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
     1561 aacaaagaat gccataaaag tcagcgcaat cagcagaatg gcgtacttag tcgcccggtc
     1621 cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     1681 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctact
     1741 ttgccattgc gcttgaaaac ctgactcgct aacttcccgt ttggctggta gaaaatcacc
     1801 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccgccagg
     1861 caccacagaa agatcgccgg taccgcttaa attcagcgcc acattcagct tcaggttctg
     1921 cttgcgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     1981 ggtgccgggt tcaatggtca gcgccgtacc gttaacttca ggcgctttca ccacaccaat
     2041 accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga tatttggcgc
     2101 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     2161 gacctgaccg gtatagatgc ctatcttgcg ttcttccaca ttctgattgc catcaaccat
     2221 caatgactca ggtaaccaaa aatgaataaa acttcgtttc cgctccacgg ttttatcgtc
     2281 ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa cgagtttttg
     2341 cggcccgctg gtactttggc gaatcacatc ttccacatcg ctacggtaat cggcacgttc
     2401 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacaa
     2461 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     2521 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     2581 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     2641 ggcgacctca ctgacaaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     2701 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatcc cactaccggt
     2761 atccagcacc ttaagggtga cgtattcctg atccacttcg gcgcttagcg ttatgcgacc
     2821 gctctcggga gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gctccagtaa
     2881 cgccggttca gcagcaacgt taacctcagt aggcataaca tgcaaagtga tgtttttttc
     2941 tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca catcaacagc
     3001 agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
     3061 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcggta aaacgagcca ccacttccgg
     3121 cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     3181 ctcatgagtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     3241 catactttcc agcgcctgcg cgagtttacg caactcgcta ctaccgagat cggggagagg
     3301 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tagagcggtt
     3361 gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccaacaaaa tggcgctggc
     3421 ccataaaatt cgccgctcgc tacgcttaat gaccggagcc atcgccgcgt tcggtttgcc
     3481 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
     3541 agaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
     3601 cgttagccag acgtcattcc agcgcgaata atcctgtcca acggctttat ttgccgaatc
     3661 gaacaatact ttgccctgcg catcggtcat atagacatga tattcgttgc gcactttgtt
     3721 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
     3781 ttgcccatgc gttgggtccc cagagagcaa atcgggacgt gccagctccg ccagcaacgt
     3841 tgcggtgtcg attaacgtcc cctcggttgc tcttcgcacg cccggtttaa cttctttgac
     3901 aaaaatagcc agaacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcaacaa
     3961 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
     4021 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcagcgtttt aatgtgggta
     4081 tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat tagttgctgg
     4141 cgggaccaga cgcggcccgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
     4201 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg caggttcatt caattcaaaa
     4261 tgaccaatac ggataacggg agacggcgtc gagaacttct tcacccgacg cagtaaggtg
     4321 cgcactctgg cgcacacttc gcgaggtgaa aacggtttag ccacgtagtc gtcagcgcca
     4381 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
     4441 aacgccggat ggagcgccag taactggcgg cacaattcaa agccgctgat atcaggcaaa
     4501 ccaacatcga gaatcatgac atcgggaacc tgctggcgag ctttatccag cacaggcaag
     4561 cctcgctcaa agacctcgac gtcaaaacct tcctgttgca acatgtagac cagcgtatcg
     4621 gctatccctt gctcgtcttc gactaaccag accgtttccc gttgcataaa tcgcctctat
     4681 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
     4741 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tagcgtcata aaagcgcacg
     4801 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
     4861 ttgccgtttt taatacgatc cgatagttca atcggcccga cttgttgaca agaaatggcc
     4921 gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt tttcgcccgg
     4981 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttcaac aacaattttg
     5041 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
     5101 aatggcccca gcattatcag gcttaaagac aggattaaat gcttgtattt catatcgtta
     5161 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
     5221 acaaaatctc aatactttta tttccgtcaa gacctaaaac atactcgact aagaaaaaaa
     5281 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
     5341 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
     5401 ggctggaagg tcatctggat catcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
     5461 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
     5521 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
     5581 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgctttca
     5641 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
     5701 gtattcgccc gccgctacgt ctgggtgaat tcactatgcc agagcacaaa tttgtcactc
     5761 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
     5821 ctgatttccg ccatgaaatg cgctaccagt tctggcacga ttttctcggc aactcgccaa
     5881 ccattccacc agtgctttac ggtttgaacg aaacacgtcc gagccaggat aaagacgacg
     5941 aacaagaggt gttctacacc accgcgttag ctcaggatca ggcagatggc tatgtactga
     6001 ctgggcatcc ggtgatgttg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
     6061 gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca atgcttaacc
     6121 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
     6181 gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt taacgctgca
     6241 gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca accacccagc
     6301 cagacgccag ccacaagctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
     6361 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccagg catcccagtg
     6421 caataccatg gctcaccagt agcggtcggc ttccctgcgg taagtcccgg caagattcca
     6481 gtgccgcatt aacgcgatcg ctgagctcct gcattgactc accttcagga atacgcccgt
     6541 caacggtgcc attgaccagt tgccgacgcc agttctcttc ttcttcggtg agtgaatcga
     6601 tatttcttgt ttccagtaca cccatattta attcacgcag gcgagaatca aagatgatgt
     6661 cacaaccgca ggcctgggcg atgatttccg ccgtgcgccg ggtgcgtcct aaatcgctac
     6721 tgatgatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
     6781 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
     6841 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
     6901 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
     6961 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
     7021 tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca gtgaggaaac
     7081 gcgcgctggc gcacgaaata gggtagccaa tgcccgccgt ttacttcctg aggctgattt
     7141 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgataga
     7201 aaacgccagc cagcgcggcg aggcgcgttc ggctactttg ccgttgcctg cggtgattct
     7261 ggaaaaagtg cgtgaaggtg aggtgcttgg tcccgtgatg tcgcgttata ccggtattga
     7321 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcac
     7381 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcataatg ccgtgtatca
     7441 gccattgcag gcctgattag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
     7501 tgcgccatgt cctatcaggc ctacaaaatc aatcgctttt cagcaacacg tcttccagcc
     7561 actggcgcag ctcgacgggc gcggctttca gactattaga tccacgcgta atcgtcgcga
     7621 tgcccgcgcc gagttcattt tttaactcac gctggctcat ttcgccgcgc aacagctctt
     7681 cgacaatacg cacgcgagtc cccaacgctt cgcgctcatc tggcgtcagc atcaggttta
     7741 acaacggtaa atggagatcg ttttggtagg cattcttaag caggtcgaca aaacgtaacc
     7801 actcctggtg acgctgttct gccatcgctg ctgaataggg tgattgttgg gccatagtat
     7861 gtcgccattg ttagcggggg aagcaaaatg cctcccccgg ttgtactcgc taaagagtac
     7921 gatagcatat cataaacgtg tggatcagta acgacgtccc cattccgtgg cgctcatcaa
     7981 cgtcggttta tcccccatga aatagcggta gtaagcgtca taagccagca cgtttttcac
     8041 ataaccgcgc gtctcggaga atggaatact ctcgacaaat gccactgcgt cgatacgccc
     8101 tgcgctgttg ccaagccagg ttcgcacccg ccctggtccg gcgttataag ctgctgagga
     8161 gaaaatacgg ttattgccaa actgctgata aacatattgc aggtaactgg tgccaatgtt
     8221 gatgtttgtt tccggatcca gcaattgccc agggctgcta taaccgggaa tagagaacat
     8281 cttcaccgta tgggtcgcgg taccaggcat aatctgcatc aggccgctgg cccctaccgg
     8341 tgatttcact ttcggattcc aggcactctc ctgacgggca atcgccatcg catagctttg
     8401 cgggatctcc ttaccactgg tgtagcgttt gaaaagatcg ttgtaagcca gcgggaatcg
     8461 ctcttccaga tgatcccaca gcttcccggc gatcgttgcc tgaacgctaa gatcccacca
     8521 ttggttattg aaagcatacc gagccagttg agcctgctct gtttttgact tgctcttcac
     8581 cagattggcc cactcgctac gcgcggtgtt atcgagattc cagtacatca actcgcgcac
     8641 gcgcgccatc tccggcccct gagtcagggc gctgtcaaca ttctgcggcg ctttatcaat
     8701 cttcagctca tactcttcgc cgatgcgttg tgcagcaacc atcgggtaga aaccacgctg
     8761 ttgcatgagt tgatgcaaaa tctcttttgc ttcagcttca cgtccgcgtt ccagcaataa
     8821 atccgcctgc cagtaacgcc attcatcttt ctctttcgct tccataggca gacgcgccag
     8881 ccaggtattc aggccgcgac gatcgccggt gccaagcgcc attcgcacac ggcgttcaat
     8941 aagcgaagta gattgcgaac gcataatcgc gtcatcgcgc catttcgcct gctcgtcggt
     9001 gacatcgttg cccatcaaac gccatgcgac aatatcgcgc agctcctgaa tctgatcttc
     9061 attaagctgc tgtgcctgag caagcgacgg gatcatcagc cgtgcatttt cagcatcctg
     9121 ccgcgccaca ctggcaaacg ccaccgccgc catttgacgg gtaaaatcgg tcgcgccggt
     9181 tgtacgcgcg aaggtcagta ccgtattagg gttgttcgcc agtgaaatga ttgccgaagc
     9241 gatagtctgg taatcggcag gcatctgccc tgccagcact gttaccaggc cggtgttacc
     9301 cgctttcatc gccagacgga tacgctctaa atacgccagc ggatcttgtt tacctgacgc
     9361 acgccagacg ctaaataact tgtcacaggc gttaggctgg ctcttgccgg ttagccacag
     9421 ctctttcgcc ccttgccagg cttcttcact ctgcccggtg ttccatttcg catagtagta
     9481 attacattgc gcttcggtag ttccgggctt ttccgggcta aaggctaaca agccacgcca
     9541 gtcttcacgc cgcgccagtt cattgacgaa acgagattgc agcgtgcgag cgggaggaag
     9601 cgtggggtta gcgcgaacaa agttagtgac cgtcaccgcc ggttgattca tcaggtcatc
     9661 agtgatctgg cggtattcca ggtagggata aagtgggtaa tctttcagtc caggcatcat
     9721 ttgttcgacc acatccattt gtcgattatc ccaggcctgc ttgatttgcg cgtaacgact
     9781 acgctgctca tccagtgagt cggctcgcgc cacgctgctg accgtcagca gacagacacc
     9841 gacagccaac agccgccagg taacgtgttt ggctttttcc acaagcgcat cctctaagtg
     9901 tcaatacatc aatgcagcgt catgccgcgt aatagattta tgctaaccag tcattgccgt
     9961 ttacgccacg ttacggacac ttttttactt ttacggggtg atcgactggc tgggattaga
    10021 cagggaaaca ggctacactt cgcctttgaa aactcatcat cacgataaac atagaggcga
    10081 agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt gttccgccga
    10141 aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa attggtgtcc
    10201 tgggtctgaa cggcgcgggt aagtccaccc tgctgcgcat tatggcgggc attgataaag
    10261 acatcgaagg tgaagcacgt ccgcagccag acatcaagat tggttatctg ccgcaggaac
    10321 cgcagctgaa cccggaacac accgttcgtg agtccattga agaagcggtt tctgaagtgg
    10381 ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta tgccgatccg gatgccgatt
    10441 ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct cacgacggtc
    10501 acaacctgaa cgttcagctg gagcgtgcgg cggatgcgct gcgtctgccg gactgggacg
    10561 cgaaaatcgc taacctttcc ggtggtgagc gtcgtcgcgt ggcgttgtgc cgcctgctgc
    10621 tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat gcggaatccg
    10681 tagcgtggct ggaacgcttc ctgcacgact tcgaaggcac cgtggtggcg attacccacg
    10741 accgttactt cctcgataac gttgcgggct ggatcctcga acttgaccgc ggtgaaggta
    10801 ttccgtggga aggtaactac tcctcctggc tggagcagaa agatcagcgt ctggcgcagg
    10861 aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag tgggtacgtc
    10921 agggaactaa aggccgtcag tcaaaaggta aagcacgtct ggcgcgcttt gaagaactca
    10981 acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca cctggaccgc
    11041 gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctatggt gatcgtctgc
    11101 tgattgactc cctgagcttc tcgatcccga aaggagcgat cgtcgggatc atcggtccga
    11161 acggtgcggg taaatcgacg ctgttccgta tgatctctgg tcaggaacag ccggacagcg
    11221 gcaccatcac tttgggtgaa acggtgaaac tggcgtcggt tgatcagttc cgtgactcaa
    11281 tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatc atgaagatcg
    11341 gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa ggggttgatc
    11401 agggtaaacg cgttggcgaa ctctccggcg gtgagcgcgg tcgtctgcat ctggcgaagc
    11461 tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac ctggatatcg
    11521 aaaccctgcg cgcactggaa aacgccctgc tggagttccc gggctgcgcg atggttatct
    11581 cgcacgaccg ttggttcctc gaccgtatcg ccacccacat tctggattac caggatgaag
    11641 gtaaagttga gttcttcgaa ggtaacttta ccgagtacga agagtacaag aaacgcacgc
    11701 tgggcgcaga cgcgctggag ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg
    11761 taaaatgccg gatgcggcgc accagcatcg catccggtat tagttaaatg attaatggga
    11821 agcaagttcc cggaaaaagg caggattttc tttaattgtg gcaagtactt ttgcagccaa
    11881 tcccgtagga tttcttcgct tcgattccca gcttttaatg gtatccacac tggttcccag
    11941 agcttttgcc atctcacttt gagaaacgtt gagttgctca cgaatagctt taacatccgc
    12001 aatttcgtag cgggtcaccc gtgcaggttc ttttaaacct tgtttaatct cgacagcctc
    12061 ttccagagag gttttcaact catcaaaaaa actcatttta tatctcgcct ttgagcagtt
    12121 tcgtcaactt ctttagttga gctttttccg tatctgtcag actatctttg gcatttatcg
    12181 gataggccat aatcaaataa ataatctctt ccgtcgccag aaagtaaatt attctgacac
    12241 tgccactttt tccctgtgaa cccgcagcca ttcggacttt acgcaatcca ccagtctgct
    12301 gaatgagatc acctttatcg ggagttccga tgagttcttt ttgtaattcc tttaattcat
    12361 catcggttgc aatctgcttg atctgacgcg taaacattgg cgtctctata aatattatcg
    12421 ccttacccat tccccctccc tggcaggtac attgtacacc taaatggagt actaagtaca
    12481 cttttaaatc agtaatccca ctcaacatct tataaagatg attatctttg ctcccccatc
    12541 atctcccgca ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc
    12601 cgcacgaatt cgatattgtt ctcttccaac atctccacca gcaagttctg gaactctttg
    12661 cgatccaccg aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc
    12721 agcaggatca ccagatcgaa acggtattca tcaatcaacg cctgcacgaa cggatgctca
    12781 cgcccttcgt actttttgca gaacgcctgg gtagtgacaa aatcggtgtc gataaacgcc
    12841 actttattgg catatttcac tgcaaaatca atatattgcg cgtgtcccag cgcgatttta
    12901 tcgtagtcgg aatactgcaa cgcaatctca tcaccgccaa ggtgtgaaaa gacataatcg
    12961 cgaccatatt cccatgcact ggtagtgttg aagatattgg caagtttgtt taccagggtg
    13021 gatttaccgc tcgactcgcc gccaaggatc gccacggtac gcacaaagaa cggcttaact
    13081 tccgtaggaa tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata
    13141 ctcataaagg tacgcttcgg atcgatcaac accgtctcga tccccagatg ttccatatac
    13201 tgcggcgcat cggcttcttc tgaggtgtag ataagatccg gctgaatgcc tttttcggcc
    13261 ataaactttt tgatgccgtt gctccacaca tcccagccgt gtggatacgg ttccatgccc
    13321 tcttcgttaa aagcatgaat gcggatattt ttttgatact taaaggtctg caataaccag
    13381 cgcagacgat ccggcacggt tggctgctgc gacatggcgc tgtcttcgaa caatgcgcgg
    13441 tcgcgggtat cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc
    13501 tggataaggt agatatgtcc agtatgcagc gggtaaaact taccgaatac gacaccgatc
    13561 gttttcttct gccggggaaa ttcaagtccc aaataacggt gcaatgcctc cagcttttgt
    13621 gcgctggggc ttttgatttt ggcattcagt aactggctta aatacccttt ggtcataccg
    13681 ctggcatcag ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa
    13741 tcaaatgacg acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg
    13801 cgtcggagag ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggcacgt
    13861 tagctgccgg aacaattgcc cggcgaaaac cgtgtttcgc cgcttctgag attcgttcct
    13921 ggccgctggg caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat
    13981 cctgcggtag cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaaatcga
    14041 cactggtttc cgtcaccttc acgccaccga ccacgttaac aaacacatcc tgatcggcca
    14101 tttgtagacc accgtgacgg tgaagcaccg ccagcaggat tgccagacgg ttttgctcca
    14161 gcccgactgc cacgcggcgt ggattcgcca tcatcgagtg atcgacgagc gcctgaatct
    14221 ccaccagcag cggacgcgtt ccttcccaca ctaccatcac tgagctaccg gaggtcactt
    14281 catcgccgcg acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgctcgg
    14341 tcattgcgaa gacacccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg
    14401 tgcgaaaacg tgagtcggca tcaccatcca aaagcaccga acagtcgata cagtgttcca
    14461 gcactttagg gccagccagc gagccatctt tggttacgtg cccgaccatg acaatcgcca
    14521 caccgcgcgt tttggcgaag cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc
    14581 tgccaggtga tgattgtaca tccgccatat gcatcacctg gatggagtca attaccatca
    14641 gcttcggttg ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacagca
    14701 tattaagatt gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg
    14761 actcttcgcc ggtgacatac agcgttttca tctgctgcgc cagtttgcac agcgtttgca
    14821 gcaacagcgt ggatttcccc gcaccagggt taccgccaat cagaatggcg cttcccggca
    14881 ccacgccgcc gcctaatacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct
    14941 cttcaaggct gatgtcggaa agtttctgga cttttgccac tccggcgcta ccggcatagc
    15001 cactgagacg ctcgttacgc gccaccgttg gcgacgctgc aagacgcacc tcggtgatgg
    15061 tgttccaggc atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt
    15121 cattacaaac aaaggcgcgt tttggagctt ttgccacgat gtacctcgtt aatgctgtgc
    15181 cgcccgcagg atgacgggcg ggcaattact tctgattcag gctgccagag aggatgcaga
    15241 atacccccat caggtcagcg tgacggatgg tgacttccgt cttttcattc acttttggct
    15301 tggcatggta ggcaatcccc agccctgccg ctttgatcat cggcaggtca ttggctccat
    15361 cgccaatcgc cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgggtcaggg
    15421 ttttcgcttt atattgcgcg tcaacgatgt cgccgatcac attgccggta aatttaccgt
    15481 ccatgatctc cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt
    15541 cagcaaagaa agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct
    15601 tgagtaccag ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa
    15661 tattggcgtc agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga
    15721 gttcgccgcg catcgcccgt tcggttacct ccgccaccct ctcgcccgtt ccggccagtt
    15781 tggcaatttc atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca
    15841 accccggcgt gcgcaggtgt gggattttcc ccagcggcgc gacatccagt tgcgcttcat
    15901 gggccaggcg cgtagcccgc gcagtgagtg aacctgccag acgaatcacc tgataatctt
    15961 ccacgcacca ggcggcaaca atcaccatag ccgcgcccag tttgctctgg tattgggtca
    16021 gacgttgttt atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat
    16081 ccagtggcat cacttcatca ccacttaatg aaagaggcag accaggccat aaagagacat
    16141 cttcaggcag gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag
    16201 cagggaaaat aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca
    16261 aattttcaaa gggtggaaga tggctcgcac aaaactgaaa ttccggctgc atcgggcagt
    16321 gattgtcctg ttctgtcttg ccttgttagt ggcgctgatg cagggggcgt catggtttag
    16381 tcaaaaccac cagcgacagc gtaatccgca actggaagaa ctggcccgca ccctggcgcg
    16441 gcaggtgacg ctaaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat
    16501 tcaggcgatc ctcgatcagt taacggatga aagccgcatt ctcgacgcag gcgtatatga
    16561 tgagcaaggc gatcttatcg cccgttctgg cgaaagcgtc gacgtgcgcg accggctggc
    16621 gctcgacggc aaaaaagcgg gcggctattt taaccagcag attgtcgaac ccattgcggg
    16681 taaaaacgga ccgctcggct atctgcgcct gacgctcgac acccatacgc tcgccaccga
    16741 agcccaacag gtggataaca ccactaacat tttacgcctg atgttgctgc tctcgctggc
    16801 aatcggcgta gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc
    16861 gcccttcctg ttaaccgcca gtaaaccggt gccggaagag gaagaaagcg agaaaaaaga
    16921 gtgacccatt actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga
    16981 ctcttacgac ccgtggttta acctggcggt agaggagtgt atttttcgcc agatgccggc
    17041 tacgcagcgc gttctgtttc tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca
    17101 gaacccgtgg aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg
    17161 gcgcagtagc ggtggcggcg cagtgttcca cgatctcggc aatacctgtt ttacctttat
    17221 ggctggcaag ccggagtacg ataaaaccat ctccacgtcg attgtgctca atgcgctgaa
    17281 cgcgctcggc gtcagcgccg aagcttccgg gcgtaacgat ctggtggtga aaaccgccga
    17341 aggcgatcgc aaagtctcag gctcggccta tcgcgaaacc aaagatcgtg gcttccacca
    17401 cggcactttg ctgctcaatt ccgaccttag ccgcctggca aactatctca atccggataa
    17461 aaagaaactg gcggcgaaag gcattacctc agtgcgttcc cgcgtgacca atctgaccga
    17521 attgctaccg gggatcaccc atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc
    17581 ccattatggc gagcgcgtgg aagcggaaat catctccccg gacaaaacgc cagacttgcc
    17641 aaacttcgcc gaaacctttg cccgccagag tagctgggaa tggaacttcg gtcaggctcc
    17701 ggcattctcg catctgctgg atgaacgctt tacctggggc ggcgtggaac tgcatttcga
    17761 cgttgaaaaa ggccatatca cccgcgccca ggtgtttacc gacagtctca accccgcgcc
    17821 gctggaagcc ctcgccgggc gactgcaagg ctgtctgtac cgcgcggata tgctgcaaca
    17881 ggagtgcgaa gcgctgctgg ttgacttccc ggaacaggaa aaagagctac gggagttgtc
    17941 agcgtggatg gcaggggcgg taaggtaact cgctgattaa aatatccagg gaaggaaata
    18001 tgatcgatct ggaaaaccag gaacgggaga ttatcaattt aatgttcagc cagggtatat
    18061 cctggctaac cgccgtgcgt atcaggcata aactatcact tgctgaagta agtaaaatgt
    18121 taggtatctc cattaattct ctaaaacaga tcgaaaagac ggaacggctt agttcaaaca
    18181 tcaaaagcaa aatggcagga atttatggtt gtccaccaga gttgctgata tgcccttcct
    18241 ggatgactgc tgaacataaa tgaatagatt cgtgaataga tacttgaatg gattttattt
    18301 tctatccata tgaaaaataa tagtaaatct ataaatcagt tgctattgat gaataaatac
    18361 taaaaagccg gagcagtctc ccactccggc ttcacacgac aatcgccttt cagcaaaaca
    18421 caattactct ttatcgccca gcagaacgga ttccagcgcg attttgatca tgtcgttgaa
    18481 ggtggtctga cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt cagacacggt
    18541 gcagatggtc agggctttcg cgccaaattc tgcagcgacg ccgtagatac cagccgcttc
    18601 catttccacg ccgaggatgc cgtatttttc catcacgtcg aacatttcgc cgtccggaga
    18661 gtagaacagg tcagcggaga acaggttacc aacgcgagca tcaacgccca gtgcttttgc
    18721 cgcgtctacc gcgttacgca ccatgtcgaa gtcagcgata gcggcaaagt catggtcttt
    18781 aaaacggatg cggttaactt tggaatcggt gcaggcaccc ataccgataa cgacgtcgcg
    18841 cagttttacg tgcggcagaa cagcgccaca ggaaccaacg cggataattt tcttcacgcc
    18901 gaaatcagtg atcagttctt tggtgtagat ggagcaggac gggataccca taccgtggcc
    18961 cattacggaa attttgcggc ctttgtaagt accagtgaag cccagcatac cgcgaacgtt
    19021 gttcacttca cgggcatctt caaggaaagt ttcagcaata tacttcgcac gcagcgggtc
    19081 gcctggcatc aaaactacgt cagcaaaatc gcccatttct gcattaatgt gtggagtagc
    19141 catcgtttta tcctttttgt gacatattaa aggcggagtc actgctccgc ccaaatccat
    19201 cagaacatgg ctttgccata ttccatatca gaagtaccaa aatattttgc cagagtctgg
    19261 ccgatatccg cgaaggtttc acgatgcccc agcgaacctg gtttcacttt cgggccgtaa
    19321 accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg tccaggtcgg gtcacaaccg
    19381 tggtcagcag tgagaatcag aatatcgtca tcgcgcagca aagacatcag ctccggcaga
    19441 cggcggtcga acagttccag acccgcagca taaccggcga cgtcgcggcg gtggccccag
    19501 gaagagtcga agtcaacgaa gttggtgaag acgatggtgt tatcacccgc ttctttcatc
    19561 tctttgatgg tggcgtcaaa cagcgcgtcc agcccggtcg ctttcacttt tttggtgata
    19621 ccgcagttgg catagatgtc tgcaatttta ccgaccgaaa ccacctggcc gtgtttttca
    19681 tcaaccagtt tctgcagcac ggtcggtgct ggcggctcaa cagccaggtc gtgacggtta
    19741 ccagtacgct ggaagttacc agctttgtcg ccgataaacg gacgagcgat aacacgaccg
    19801 atgttgtagc cgccgttggt cagctcttca cgggcgattt cgcacagctc gtagagttta
    19861 tccaggccaa aggtttcttc atggcaggca atctggaaca cggagtcagc ggaggtatag
    19921 aaaatcggct tgccggtttt catgtgctct tcgcccagtt gatccagaat gaccgtaccg
    19981 gaagagtggc agttaccgag gtaacccggc agattagcgc gttcaaccag tttatccagc
    20041 agctcttgcg ggaagctgtt ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg
    20101 acaccggcga tttcccagtg accagacggg gtgtctttac cggatgacat ttcgtgcgcc
    20161 catgcgtacg cgccgataac ttcagcgttg ccatccattc ccgccggaat gaaaccggta
    20221 gaaccttcgt gtgctttcgc cagcccaaga cgggtcagat ttggcagatt gagcgggcct
    20281 ttacgaccgt ggtcggcttc gcctttggca caagcttctg cgatatgacc cagggtgtca
    20341 gccccgacgt caccaaagcg ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc
    20401 accataataa atgcacgttt catatgttct ccgtactcag tgcttcaaat ttaaatgatc
    20461 ggatcagtat accgttattc gctgatacgg cgatagacag ttggtgtgct ttccggtgct
    20521 ttatcggcaa gtttaattgc cgctttcacc gctttcgccg cttcctgcca gctgttttcg
    20581 tctttcgcgt ggataactgc cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc
    20641 atatcagtaa agccgacgct gtaatcgatg gtgtcagatg cctgacggcg tccgccgccc
    20701 attgcaacca ctgccatccc cagcgcgcgg gtatccattt cactgacaaa cccttcggta
    20761 tcagcataga ctgctttcgt cagcatcgct gtcggcagat acttcgcgta gttctcaacg
    20821 aagtcggtcg ggcctttttg tgccgctacc atacgaccaa agacttctgc cgctttaccg
    20881 ttgtccagca ccgcctgtaa tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg
    20941 gagatcagca tctccacgca cagtgccatc gtgacatcaa acagacgcgg gttacgatat
    21001 tcacccgtca ggaactgcac cgcttcacga acttcaaccg cgttacctgc actggaggcc
    21061 agtacctgat tcatatcggt gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg
    21121 ccaacaatcg cttcggcaag ggcttcagag agttcgtagg tcggcataaa cgcgccgcta
    21181 cccactttca cgtccatcac cagcgcgtcc agaccttccg ccagtttctt cgccagaata
    21241 gaggcggtga tcagcgggat tgaatccacg gttgcggtaa tatcgcgggt cgcgtagaaa
    21301 cgtttatccg ccggagccaa tgagctggtc tggccgataa tcgccacgcc gacgtcttta
    21361 ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga agccagggat ggattccagt
    21421 ttgtcgagcg taccgccagt atgaccgagg ccgcgaccgg agatcatcgg aatatagccg
    21481 ccacaggcag cgaccatcgg ccccaacatc agcgaagtca catcgccgac accgccggtc
    21541 gagtgtttat caacaatcgg gccattcaga tgcaggcttt tccagtcgag aacggttcct
    21601 gaatctcgca tcgccatggt cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa
    21661 atggtcatcg cgagggcggc aatctgccct tcggagatag tgttgtcgcg aatgccgttg
    21721 ataaagaaac gaatttcttc atcactcagc gcatgaccat cacgtttttt acgaataatt
    21781 tcttgtgcga gaaacaaggt aacctcccgt ggaaaagaga cgaggtaatc ggggaaccgg
    21841 atgagcatcg ctgcatcatc cggcaaatca agccttagta gctgctggcg ctcttaccgt
    21901 cgccgtggcc cagagctttc agcaggcttg ccagcaagct ggaagcacca aagcggtagt
    21961 gacgcgcatc agcccagtca gcaccgaaca gttcatctgc aatggcgaga tatttctgcg
    22021 catcttccgc agtacgcacg ccgcccgccg gtttgaaacc aacggttttt tctacgccca
    22081 tatcgcggat cacttccatc atgatgcgcg cgctttccgg cgtcgcattc acagccactt
    22141 taccggtaga ggttttgatg aagtcagcac ccgctttgat ggagatttca gacgctttac
    22201 ggatcagcgc ttcgtctttc agttcgccgg tttcaataat cactttcagc agtacgttcg
    22261 ccgcagcgca agcctcttta caggctttca ccaggtcaaa accaacctgc tcgttacccg
    22321 ccatcagcgc gcggtacggg aacaccacgt caacttcatc ggcaccgtag gcgattgccg
    22381 cacgggtttc tgccagcgca atgtcgatgt cgtcgttacc gtgtgggaag ttagttaccg
    22441 tagcgatacg gatttccggg gtgccctgct ctttcagagt tttgcgagca atcgggataa
    22501 agcgaggata gatacagata gcggcggtat tgccgaccgg agttttggcc tgatgacaca
    22561 gggcgatcac tttctcatcg gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca
    22621 gtgcacgcag gctgcttgct ttcagatcag tcatttcatt ctcctggctt gtcgccaata
    22681 aaattcacct tgcgagtttg ttagtattct aacatctact ccgcaacaca cttcgatgta
    22741 catcacaatt aaggaaatct acttacaagt ttgcatcact gtaatgcgat ctggttcaaa
    22801 taattcactt tcaaatgaat gcgtcagagg tggcaaacgt atcaggatca aagtgaacat
    22861 tacaaaactt cttacaatgg cgcatcacct tcggcataaa aggaaaagaa gatgcccact
    22921 tctcatgaaa atgcactgca acaacgttgc cagcaaattg tcaccagccc ggtacttagc
    22981 ccggagcaga agcgccattt tctggcactg gaagcagaaa ataatctgcc ttacccacag
    23041 ctgcctgccg aagcccgccg cgcgctggat gagggggtaa tctgcgatat gtttgaaggt
    23101 catgcgccgt acaaaccgcg ctatgtctta cccgattacg cccgttttct ggcgcacggt
    23161 tccgaatggc tggagctgga aggcgcgaaa gaccttgatg acgcactctc tctgctgacc
    23221 attctttacc accatgtacc gtcggtgaca tcgatgccgg tctacctggg gcaactggat
    23281 gcgttgctgc aaccgtatgt tagaattcta acacaagacg agatcgatat tcgaataaaa
    23341 cgtttctggc gttacctcga cagaaccctg ccagacgcct ttatgcacgc caacatcggc
    23401 ccgtctgatt cgcccattac ccgcgcgatc ttacgtgcag atgcagagct gaagcaggtt
    23461 tcaccgaacc tgacctttat ctacgatcct gatatcaccc ctgatgacct gctgctggaa
    23521 gtggcgaaaa acatctgtga atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat
    23581 aaaattttca caaaaggggg ctacgggatt gtgagctgtt acaactcact gccgctggcg
    23641 ggtggtggca gcacgctggt acgccttaac ctgaaagcca ttgccgagcg cagcgaatcg
    23701 ctggatgact tctttacgcg cactctaccg cactactgcc agcagcagat cgccatcatc
    23761 gatgcgcggt gtgaattcct ctatcaacaa tcacacttct ttgagaatag cttcctggtg
    23821 aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt ttggcatgta tgggctggcg
    23881 gaagcggtta acttgctgtg tgagaaagaa ggaattgtcg cacgttacgg taaagaagcc
    23941 gctgcaaatg aagtgggtta tcgcatcagc gcacaactgg cggagtttgt cgccaatacc
    24001 cccgtgaaat atggctggca aaaacgcgcc atgctacacg cacagtcggg gatcagttcc
    24061 gatatcggta ccacgcctgg cgcacgcttg ccgtatggtg atgagccaga tccgattacc
    24121 catctgcaaa ctgtcgcccc gcatcatgct tattattatt ccggcatcag cgacattctg
    24181 acgctcgacg aaaccatcaa acgtaatccg caggcgctgg tacagctttg cctcggtgcc
    24241 tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg gtaacgatct ggttcgcgtt
    24301 accggttata tggtgcgttt gtcggattta gaaaaatatc gcgccgaagg ttcacgcacc
    24361 aacactacct ggctgggaga agaagccgca cgcaacactc gtattctgga acgccagccg
    24421 cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt aagattatcc ccttctcctg
    24481 cgttgacggg ccaggcagtc gtctggctct gtttttgcag ggctgcaatc tgcgctgcaa
    24541 aaactgtcac aatccgtgga cgatggggcg ctgtaatgat tgcggagagt gcgtgccgca
    24601 gtgtccgcat caggcgttgc agattgtcga cggcaaagtg gcgtggagcg cggctgtctg
    24661 cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat gctacgccaa tggcgcaatc
    24721 catgagcgtg gacgaggtgc ttaaccatgt ccgcaaagcg gttctgttta ttgaagggat
    24781 aacggtaagc ggcggcgaag ccacgaccca gttgccgttt gtggtggcgc tgtttactgc
    24841 catcaaaaac gatccgcaac tgtgccatct cacctgcctc gtggacagta acggcatgtt
    24901 gagcgaaacc ggctgggaaa aattgctccc ggtgtgtgac ggcgcaatgc tcgatctcaa
    24961 agcgtggggg agcgaatgtc atcagcaatt aaccggacgc gataatcagc agatcaagcg
    25021 cagcatctgt ttgctggcag agcgcggtaa gctggcggaa ctgcgtttgc tggtaatccc
    25081 tggccaggtg gattatttgc aacacatcga agaactggcg gcgttaatca agggacttgg
    25141 cgatgttccg gtacgcctga acgcgtttca tgcccacggc gtgtatggcg aggcgcaaag
    25201 ctggccgagc gccacgccgg aggatgtgga acaactggct gatgcgttat gggagcgcgg
    25261 ggtgagccgg ttaatatttc cggcgctata tttgtgacat gccggatacg gcctacggat
    25321 cggcacagac ctgtaggcat gataagacgc gtcaagcgtc gcatcaggca ttgtgcgcca
    25381 gctgccggat gcggcgtgaa cgccttatcc ggcctacggt tcggcacaga cttgtaggca
    25441 tgataagacg cgtcagcgtc gcatcaggca ttgtgcgcca acggccggat gcggcgtaaa
    25501 cgccttgtcc ggcctacggc acgttaaaca acgtataggt gttattaagc aacacttcgg
    25561 caatctcatc cgccggttcc ggtcgtaact cgcaaagcac ggcaaaaaca cgggcggcct
    25621 gttcagggcg attaggctgc ccctgaaaac cattgagcgg catatctggt gcgtcggttt
    25681 ccagcaacaa cgatgccagc ggtaattttg cgatgacatc gcgggtttta ctggcgcgtg
    25741 gataggtgat agtcccgcct acgccaattt tgtagccctg ttgcacaaac cgttcggcct
    25801 gttgcaggct gccggaaaaa ccatgaacca cgccagtgca cggtaaatcg tggcgtttaa
    25861 gatgcatcgc cagtttgtcg tgcgtgcgtc gtgaatgcag gatcaccggc agatcgtagc
    25921 gtttcgccag tttcagttgt tcgtcgagta accactgctg cctctcaaat tgcggatcgt
    25981 cgccaaagag atccagaccg atctccccca ccgccaccac cttcgccgga tgcctttcca
    26041 gcgcctgctg tagctgatca agagacacat cgctatgttt ttccaacata ccaggatgca
    26101 agcccaatgc ggcatacagc ggttgatatt tttccgctaa cgcctgcacg cgggcaaaat
    26161 tttccgcctc tgtcgccgga acaatgatct tgcctacgcc cgcttgtgcc gcgcgttgca
    26221 ggctggcctc ttcatcgcca ctaaacggcg ggaaatcaaa atggcagtgg gtgtcgataa
    26281 aacggcaaat caagccagat cctcattgtt aaatgtcggg tcgttcgcct gcggagcatc
    26341 gctcacttcg gcaaccagcg tatcgttggc gactggcgca ggcggaataa cgatcgactc
    26401 cggcgtcacc accggcacca gatggcggct cagcggcgct ttttcagtca ataatttacc
    26461 gaccgttgcc agaaaataac gcccgcataa acgtccaagt ttatagtctt cacgcagcgc
    26521 cggaatccga ctgccgagcg cgatactatg caatggcttc ggcggataaa tttcgaatat
    26581 ccgcagctgg cccggtggtt tctcaataaa ttgctgaatc tcacgatagc ttgtttcatg
    26641 atgctgcacc agattgacca gcggctgcag gctactgtca cccagccagc gttccatgcg
    26701 tttgaaccac tgcggcgtgt agtacatttg tgacggcaca gtgcggatga cgaccaacgt
    26761 ttttgctccc tgccttgccg cctctttaac cgggatcgca tcactgatcc caccatccag
    26821 gtagttaata ccttccagcg acactccgct acgataaaag ccaggtatcg cactggaggc
    26881 gcgaatcaca tccagccagt tttgtttagt tggcaaaaag taattcggcg catagtcatc
    26941 ctgacgacag gcgcacatat aaaacgattt gccgctgtca aataaccgcg ctgcggtatc
    27001 catttgcaac ggcatctggc ttgcggtggc ctccaccagc caatcgagat cgataagatt
    27061 tcctccacgg acaaagcgca atggatcgaa aaattcgcgt tttgtggtgt aacgcatgat
    27121 gactttgcgc gcgtaaccgg gctgattgca gatatacgcc gagaggttct gcgccccggc
    27181 agatgtgccg agataaagat cgaaaggatt aaactgcgcg cgcataaact catccagcac
    27241 gccagccgtg aaaattccac gttgtccgcc gccttcgcac accagagcaa tccgtccagg
    27301 ctggaacggc cttagcgaca acggcgcaat attaccaagc gttacaggta ttcgctgccc
    27361 cacctctgcc ttcctgtttt tgttgtgaaa gaatgacaaa gtaacgcaat ttattattcg
    27421 ctaaaaccgc aaaagccagt ccgctggact ggctttggat aaaattttct taatggtctg
    27481 aaatctaggg tcgttttcgg cccatgaaca aactcaccag gaacagaata atcccgacga
    27541 caaagacaat tttagctgcg cctgcagcag taccggccag accaccaaac ccaagtgcgg
    27601 cggcgattaa cgcgataacc agaaatatga tgccccaacg aaacataagc gtctccttta
    27661 ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca ctcaatgcga tatttttagt
    27721 gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggtcg aattacgacg aattacttag
    27781 ttttcagatc atttttaacg cttttcacac catctaccgc tttggcgata ctttcagcac
    27841 ggtcgctctg tgcctgagaa tctacggtac cggagagctg gaccacgcca tcggtggttt
    27901 caactttcac atgacgggaa gggacaatgt catctgccag cagtttggct ttgatttcac
    27961 tggtagtggc ggtgtcaccc gcgtagcctt tcacagagcc ttctttagcg tcgcgaacgt
    28021 gcagtttgtc gctgacagag gtcacgccct caacaccttt cgccactttc accgcctctt
    28081 cggcctgagc ctggctttca acgaaaccgc tcagggtcac gactttttga tcggttttga
    28141 cagagatatc ggtgctcttg atgttgtcat ggtccaccag ggctgccttc actttcgcgg
    28201 tgatggcact gtcatccatg aaattaccga ctttattcat agagctatcg actttttgcc
    28261 ctgcgctttc attggtagtc tgcgcgttgt tttctgcgta ggcagagccg gtcgcgacgg
    28321 cagaggtcaa cattacagcc agcagagttt tcgaaatctt cagtcttgtc atagtcatcg
    28381 atttattcct gtatgtttgc tcgtaatttg agcctggcaa cacgaggttg cattgctgaa
    28441 tagggagaga cttcaccctc tacagaagtc aatggtcgcc atcacaaaag cgatgggtga
    28501 tgaataacga ccattacagc ctcttaatca gttattaata tcggtagaat gacaatcgac
    28561 ggctttagat actgatatct
//
LOCUS       DAZFRG010000036        28191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFRG010000036 DAZFRG010000000
VERSION     DAZFRG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.036"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..124
                     /locus_tag="ACHKUC_001229"
     CDS             <1..124
                     /locus_tag="ACHKUC_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617515.1"
                     /translation="DKGNGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            236..1030
                     /gene="panB"
                     /locus_tag="ACHKUC_001230"
     CDS             236..1030
                     /gene="panB"
                     /locus_tag="ACHKUC_001230"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO6617516.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1042..1893
                     /gene="panC"
                     /locus_tag="ACHKUC_001231"
     CDS             1042..1893
                     /gene="panC"
                     /locus_tag="ACHKUC_001231"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO6617517.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(1975..2859)
                     /gene="rpnC"
                     /locus_tag="ACHKUC_001232"
     CDS             complement(1975..2859)
                     /gene="rpnC"
                     /locus_tag="ACHKUC_001232"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO6617518.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGTPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIQQGRQEVSQEFALRLLSKGMSRE
                     DVAEMANLPLAEIDKMIN"
     gene            3133..3513
                     /gene="panD"
                     /locus_tag="ACHKUC_001233"
     CDS             3133..3513
                     /gene="panD"
                     /locus_tag="ACHKUC_001233"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO6617519.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVNMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3517..4746)
                     /gene="yadE"
                     /locus_tag="ACHKUC_001234"
     CDS             complement(3517..4746)
                     /gene="yadE"
                     /locus_tag="ACHKUC_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6617520.1"
                     /translation="MYKQAVILLLMLFTASVSAALPAHYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4810..5250)
                     /gene="yadI"
                     /locus_tag="ACHKUC_001235"
     CDS             complement(4810..5250)
                     /gene="yadI"
                     /locus_tag="ACHKUC_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6617521.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5355..6125)
                     /gene="yadH"
                     /locus_tag="ACHKUC_001236"
     CDS             complement(5355..6125)
                     /gene="yadH"
                     /locus_tag="ACHKUC_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6617522.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6122..7048)
                     /gene="yadG"
                     /locus_tag="ACHKUC_001237"
     CDS             complement(6122..7048)
                     /gene="yadG"
                     /locus_tag="ACHKUC_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6617523.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            7157..7819
                     /gene="can"
                     /locus_tag="ACHKUC_001238"
     CDS             7157..7819
                     /gene="can"
                     /locus_tag="ACHKUC_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO6617524.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7860..8396)
                     /gene="hpt"
                     /locus_tag="ACHKUC_001239"
     CDS             complement(7860..8396)
                     /gene="hpt"
                     /locus_tag="ACHKUC_001239"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO6617525.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8602..10992
                     /gene="gcd"
                     /locus_tag="ACHKUC_001240"
     CDS             8602..10992
                     /gene="gcd"
                     /locus_tag="ACHKUC_001240"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO6617526.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDPLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDTKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11039..12589)
                     /gene="cueO"
                     /locus_tag="ACHKUC_001241"
     CDS             complement(11039..12589)
                     /gene="cueO"
                     /locus_tag="ACHKUC_001241"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO6617527.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPVPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAVAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12755..13102
                     /gene="yacC"
                     /locus_tag="ACHKUC_001242"
     CDS             12755..13102
                     /gene="yacC"
                     /locus_tag="ACHKUC_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO6617528.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13208..14074
                     /gene="speE"
                     /locus_tag="ACHKUC_001243"
     CDS             13208..14074
                     /gene="speE"
                     /locus_tag="ACHKUC_001243"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO6617529.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            14090..14884
                     /gene="speD"
                     /locus_tag="ACHKUC_001244"
     CDS             14090..14884
                     /gene="speD"
                     /locus_tag="ACHKUC_001244"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO6617530.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14922..15284)
                     /gene="yacL"
                     /locus_tag="ACHKUC_001245"
     CDS             complement(14922..15284)
                     /gene="yacL"
                     /locus_tag="ACHKUC_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO6617531.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15459..18056)
                     /gene="acnB"
                     /locus_tag="ACHKUC_001246"
     CDS             complement(15459..18056)
                     /gene="acnB"
                     /locus_tag="ACHKUC_001246"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO6617532.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18411..20168
                     /gene="yacH"
                     /locus_tag="ACHKUC_001247"
     CDS             18411..20168
                     /gene="yacH"
                     /locus_tag="ACHKUC_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO6617533.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHTAPVITRDTQRQAAAQRFNEAEHNGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAAREKMQTNPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(20410..21834)
                     /gene="lpdA"
                     /locus_tag="ACHKUC_001248"
     CDS             complement(20410..21834)
                     /gene="lpdA"
                     /locus_tag="ACHKUC_001248"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6617534.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22159..24051)
                     /gene="aceF"
                     /locus_tag="ACHKUC_001249"
     CDS             complement(22159..24051)
                     /gene="aceF"
                     /locus_tag="ACHKUC_001249"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO6617535.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24066..26729)
                     /gene="aceE"
                     /locus_tag="ACHKUC_001250"
     CDS             complement(24066..26729)
                     /gene="aceE"
                     /locus_tag="ACHKUC_001250"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO6617536.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26890..27654)
                     /gene="pdhR"
                     /locus_tag="ACHKUC_001251"
     CDS             complement(26890..27654)
                     /gene="pdhR"
                     /locus_tag="ACHKUC_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO6617537.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(28110..>28191)
                     /locus_tag="ACHKUC_001252"
     CDS             complement(28110..>28191)
                     /locus_tag="ACHKUC_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO6617538.1"
                     /translation="GKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT         6803 a         7652 c         6913 g         6823 t
ORIGIN      
        1 ggataaaggc aacggtacct cacaacctct tcatttccag gctacattaa aacaagacgg
       61 taatattgct atagaaccgg gggattttaa agccaccagc acttttcagg ttacctaccc
      121 ctgataacac tatcgcaccg ccagacaacg gcggtgcgat acggataaat cattgccccc
      181 accaatgtga ctgataaaat gcgagacagc acaacatcat ttatcaggat acgctatgaa
      241 accaaccacg attgcttcat tgcagaaatg caagcaggat aaaaagcgtt ttgcgaccat
      301 caccgcttac gactacagct tcgctaaact gtttgccgaa gaaggactta acgtcatgct
      361 ggtaggcgat tcactgggca tgacggttca ggggcatgac tccacgctac cggttaccgt
      421 tgctgatatc gcctatcaca ccgccgctgt gcgccgcggt gcgccaaact gccttctgtt
      481 agcagacctg ccatttatgg cgtatgccac gccggaacaa gcctttgaaa acgccgcaac
      541 ggtaatgcgt gccggagcca acatggtcaa aattgaaggc ggtgagtggc tggtggaaac
      601 cgtacaaatg ctgaccgaac gtgccgttcc agtttgtggt catttaggcc taaccccaca
      661 gtcagtgaat attttcggtg gctacaaagt ccaggggcgc ggcgatgaag cgggcgatcg
      721 actgctcagc gatgcattag ccttagaagc tgctggggca caactgctgg tactggagtg
      781 cgtgccggtt gaactggcaa aacgcattac cgaagcactg gcgatcccgg ttatcggtat
      841 tggcgcaggc aacgtcactg acgggcaaat cctcgtgatg cacgacgcgt tcggcattac
      901 cggcggtcat attcctaaat tcgccaaaaa tttcctcgcc gaaacgggcg acatccgcgc
      961 ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt tacccgggcg aagaacacag
     1021 tttccattaa ggagtgacgt tgtgttaatt atcgaaaccc tgccgctgct gcgtcagcaa
     1081 attcgccgcc tgcgtatgga aggtaagcgc gtggcgctgg tgcccaccat gggtaacctg
     1141 cacgacggcc atatgaagct ggtcgacgaa gccaaagccc gcgccgatgt ggtcgtcgtc
     1201 agtattttcg ttaacccaat gcagtttgac cgcccggaag atctggcacg ttatccacgg
     1261 accttgcaag aggactgcga aaagctgaac aaacgtaaag tggatttagt tttcgcgcct
     1321 tcggtaaaag agatctaccc gaacggtact gaaacccaca cctatgttga tgttcccggt
     1381 ctttccacca tgctggaagg tgccagccgt ccgggacatt ttcgcggcgt ttcgactatt
     1441 gtcagcaagc tgttcaacct ggtccagcca gatatcgcct gcttcggtga aaaggatttt
     1501 cagcaactgg cgctgatccg caaaatggtt gccgatatgg gcttcgatat tgagattgtc
     1561 ggcgtaccga ttatgcgcgc gaaagacggt ctggcgctca gttcccgtaa cggctatctg
     1621 acggcggaac aacgtaaaat tgcgccgggt ctctacaaag tcttaagttc aattgccgac
     1681 aaattgcagg ctggcgaacg ggatctcgat gaaattattg ctattgccgg gcaagaactg
     1741 aatgaaaaag gcttccgcgc cgatgatatt cagattcgtg atgccgacac attgctggaa
     1801 gtctctgaaa acagcaaacg cgcagtgatt ctggtagccg cctggcttgg cgatgctcgc
     1861 ctgatcgaca acaaactggt cgaactggcg taatgcttaa ctggcgctac ggctgatggc
     1921 gccagttatt cattgccacg ttatctgtag gcaattattc ataatgcaga caacttagtt
     1981 aatcatttta tcaatctcag caagaggtaa atttgccatc tctgcaacgt cttcccgaga
     2041 cattccttta ctcagaagac gcagggcgaa ttcctgactt acttcctgtc ttccctgctg
     2101 aatccccttc tcttcaaacc actgcgccag cgtcatcata gactcccctc ccgtttccct
     2161 gtctctcagc acaccataaa acaaatccgc ttgttcagta tgaccgcgtt gcagcatata
     2221 gttttgcatg gcaactaact gacttccgct agtgtaccct tcgtcaatca gcgtgaccag
     2281 ttgctccagc aataacatta agtcgcgctg gcgaatatgt ttttgcagca gttcgagaat
     2341 cgcaatccgc cgatgttgca tgatttcgtc atccggtgtg atagtgatat ccaccagcgg
     2401 gaaaggactg ttatagacgc gtcgcgccag ttccggtgag taaaacatat caaaccagca
     2461 cattgatagc ggataaggtg tggcctcgcc ctgataaaac agaattggca ccaccagcgg
     2521 cagcttatcg tggtcagcct ccagatgccg gtgcatggca gcaatagaat aacgcatcat
     2581 gcgaaaggcc attttcttat ccggcttgct ttgatgttca atcacaacat gcaaataacc
     2641 gggggtgccc tgcatttgca cggaataaag cacgtcagtg ctgtgccctt tcaggctttc
     2701 ttcaataaaa ctccccgact caagatgcag cgtgtcgagg tcacacagtt cgcgtagctc
     2761 cgctggcaaa tggatatcca gaaagtcgcg agccgtttcc gcatgcatta aaaattgttt
     2821 aaacaccgca tcatgcggtg tggtactcgg tgcatccatc ttccctgcct tccgtgttgt
     2881 cttgcgtgaa ggcagtatcc agtcaaggcg caccgtcgtc agccgcctct ttagtgttta
     2941 ccgagcagcg ttcagagaaa tatcaattcg ttacaggcgt tacatggcac gcttcggcac
     3001 ggtacgcaaa ctgctgatgc aaatgccgtc agatgagtag acactaaaca aaaatcgggc
     3061 aatactgcgt gagaatttcc tcaaagcagg catcgcctgc ttcgttaacg acagggtaga
     3121 aaggtagaag ttatgattcg cacgatgctg cagggcaaac tccaccgcgt gaaagtgact
     3181 catgcggacc tgcactatga aggttcttgc gccattgacc aggattttct tgacgcagct
     3241 ggtattctcg aaaacgaagc cattgatatc tggaatgtga ccaacggcaa gcgtttctcc
     3301 acctatgcca tcgcggcaga acgcggttcg agaattatct ccgttaacgg tgcggcggcc
     3361 cactgcgcca gcgtcggcga tattgtcatc atcgccagct tcgttaacat gccagatgaa
     3421 gaagctcgca cctggcgacc caacgtcgcc tattttgaag gcgacaacga aatgaaacgt
     3481 accgcgaaag cgattccggt acaggttgcc tgattgttat ccctgcggct ggttactcac
     3541 cagccgcgac atcgtctcca gcgaatccgt tcttaagata taaagtcgtt ttagtaacaa
     3601 cggattatcc cccggtttta ctttgccttt catggtcgtc accgccaggt gaaatccggc
     3661 ttcttttgct gccttcacgg ctttgtcatt aaatccgcca aatggataag aaagatacaa
     3721 aacatgtgga ttaaattgcg ccagagcgcg gcgtgaacgt gcaaaatcaa acagaatatt
     3781 gtgctcacta cggctcagca atataggccg ccgataacca tctatccgat gcaaaaaatg
     3841 agtatgtgac tggaaatcaa atacatcgcg aatttcgtta agttcagaaa cgctcataaa
     3901 ttgcagcgat tttgggttcc atttctgcgg gtgacgtttg atgcgcgagg taacaataaa
     3961 cgccgtcgcc ttcataccat attgtttcaa cacaggatac gcatagcggc tcaccgactt
     4021 gaggccatca tcaaaggtaa ttaccaccgc tcgcgcaggg agattgatct tattcttcac
     4081 gtaaccttcc agctgcgcca tgctcagtgt cgcgtatccc ctgtcgcgca gccaggccat
     4141 ctggttattg aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac gggtgttttc
     4201 ttcgtcgcgc aaaatatggt gataggttag caccggcaag ccattatcgg gttgggcatc
     4261 cagcgcgctg atataggcca gtcgatcgcc aatacggatc tgataccagg tttgatttaa
     4321 cctgtctttc agtttatgca aaatcgggta gcgcaaattg tccgccagta ctccaaatgg
     4381 cgcactgccc acactcggtg cgttataaac cggcgtatct ttccaggtta ttaagttctg
     4441 attactcagc ggcttgttga ggtcacccaa accatcttca actttttgtc gcccctgaac
     4501 cggctcgaga tggcctttat cgataaaccc tttgccaaag ccaaaattaa atacgtaata
     4561 acttgcggca gtgggctcca cggcaatgat ttgtccggcc cgaatattcc ccaccgtcac
     4621 catcttgtca ccaatttgcg cccagaccgc ggcattttcg atggtttgca tataatgggc
     4681 aggtaatgcg gcactgacgc ttgcggtaaa cagcatcagc aggagaataa cagcttgttt
     4741 gtacatgaaa aaataaccga gggtgaaaca acgcttttat tgtagcaaaa gcgtcatcag
     4801 aatcgcgggt taatactcat acaaattatg tttgaggacg aaaggcggat ttttttgttg
     4861 ttggtgccaa agactactca cctccggcgc acccagttcg acaatatgct cgcgaaacgc
     4921 tgaactggtc atggtttcac ggcaagccat catctgttca attaacggta acgtgacacc
     4981 agaaatcact tcgcaacggg agtgtttgtg gcttaataat gaagccacgc ggtacggtgg
     5041 cgctccggct atatcggtta aaaagataac gccctcacca gagtccgttt catgcaaggc
     5101 atcgcacatc atgcggctga gcatattaga gcttaatccg cgccagaaat tcacggcccg
     5161 gcactgaaga agtgccccat gttttttctc cagcgcatcc agtatctctt gcgcccggtc
     5221 atcgtgacag gtaattaccc aacctaacat cgccttcctc cttagccgaa aatcagtcta
     5281 ccctgaccat tgttgataaa gggtgatagc cgtcaaaact cctctccccc aaatccagag
     5341 gagaggaaat agccttagct acgcaaacca cgtccacgtt ggatcagcga ccaacagatc
     5401 aaataaaacg ccacaataaa gaccaccagt acgccaaagg tagtgaccag cggaacatca
     5461 ttgataccga ggaagccgta gcggaaacca ctgatcatat aaacgattgg gttcaggtgc
     5521 gacagccctt gccagaacgg cggcaacaaa gtcagggagt aaaacacccc gcccaaatac
     5581 gtgagtggcg ttaacacaaa ggttggcacc aggctgatgt catcgaacgt tttggcaaac
     5641 acgccgttca gcaaacccgc aagggagaat agcaccgcag tgagcaccag ggttaaggca
     5701 acgaataccc acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc cgtcaccaaa
     5761 ataccaacaa acagaccacg cgccacgcca ccaccgacat atccggcaat aatgacatga
     5821 gtcggaaccg gcgctaccag cagctcttca atattacgct ggaacttggc accaaaaaat
     5881 gatgacgcga cgttagcgta ggcgttggtg atcaccgaca tcatgatcag ccccggtacg
     5941 atgaactgca tgtagctgaa gccatgcata tcgccaatac gcgaaccaat caggttaccg
     6001 aagataataa agtaaagggt catggtgatg actggcggca ccagcgtctg cacccagata
     6061 cgcataaagc gatggatctc tttcgcccag atgcttttta gcgccaccca gtaaagatgc
     6121 atcatgtgcg atctccttgt ttttcattaa ccagtgaaac aaacagctct tccagacggt
     6181 tagctttgtt acgcatactt aatacctgaa tgccctgctc acttaactgc gtaaatacgc
     6241 tgttgatccc ctgctcacgc agcacttcaa cttccagcgt ggcggtatct accagtcgat
     6301 actgatagcc atcgagcttc ggtaacggac ttttcggtgc gagatcgaga ataaaggttt
     6361 ctgatttcag cttcgccagc agcgccttca tcgaagtatt ttccaccaac tcaccgtgtt
     6421 gaataatgcc gatattgcgg cacagcattt ctgcttcttc caggtagtgt gtggtgagaa
     6481 tgatggtggt gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag cggcgaagtt
     6541 caatatccac gcctgcggtc ggttcgtcga gaatcagtag tttaggttca tgcattaacg
     6601 cacgggcaat cattaaacgg cgcttcatcc cgccagataa catacgcgca cgttcgttgc
     6661 gttttcccca tagatcgagt tgtttaagat acttttcgct gcgaatgtac gcttctttgc
     6721 gctccacacc gtagtaccct gcctgattca ccacaatttg ctgcacggtt tcaaacgggt
     6781 tgaagttaaa ttcctgtggc accagaccca actgacgttt agcgtttacg acatctttct
     6841 cgagatcgta accaaatacg ctgacccgcc cggaggtttt atttaccaga gagctgataa
     6901 taccgatagt ggtcgatttt ccggccccgt tcggcccgag aagcgcataa aaatcacccg
     6961 cttcgacctg caaatcgatt ccacgaagcg cctgaacgcc gcctggatag gtttttttaa
     7021 gctgttgaag ttccagtgca atggtcataa atttttactt accttacgtt cttacacttt
     7081 atctgtggtt taaatcgtcc cggagttgcc ctatattagc caaacgtaat tatttggtta
     7141 caggtcgtta acctccatga aagacataga tacactcatc agcaacaatg cactatggtc
     7201 aaaaatgctg gtggaagagg atcccgggtt ttttgagaaa ctggcacaag cgcaaaaacc
     7261 gcgctttcta tggattggat gttccgacag tcgcgttcct gcagaacgtt taaccggtct
     7321 tgagccgggc gaactctttg ttcaccgtaa tgttgctaac ctggtcattc acaccgacct
     7381 gaactgcctt tccgtggttc agtatgcagt ggatgtactc gaagttgaac acattattat
     7441 ctgtggccac tacggttgcg gcggcgtaca agccgcagtt gaaaacccag aactggggct
     7501 tatcaacaac tggctgctgc atatccgcga tatctggttc aaacatagct cattgctcgg
     7561 cgaaatgccg caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca tggaacaggt
     7621 gtataacctg ggccactcca ccattatgca atcagcgtgg aaacgcgggc agaaagttac
     7681 cattcacggc tgggcctacg gcattcacga cggcttgctg cgtgatctgg atgtcacagc
     7741 gactaaccgc gaaacccttg agcaacgtta ccgtcacggg atctccaacc tcaagctgaa
     7801 acacgccaac cacaaataaa aatgccatgc cggatgcaac acatccggca acttcacact
     7861 tactcgtcca gcagaatcac tttgccgata tacggcagat gacggtaacg ctgtgcgtaa
     7921 tcaatgccgt aacccaccac aaactcatcc gggatcgaga aaccgataaa ttctaccggg
     7981 acgttcactt cacgacggga cggtttatcc agcagcgtac aaatcgccaa cgacttcggt
     8041 tcgcgcaggc ttaagatctc acgcactttc gacagtgtat tccccgagtc gatgatatct
     8101 tcaacaatca gcacgtcctt gccacggata tcttcatcca gatctttgag gattttcaca
     8161 tcacgggtgg tggacatgcc gctaccgtag ctggaggcgg tcataaagtc gacttcatga
     8221 gatacctgaa cttcacggca caggtccgcc ataaacataa atgagccacg cagcagaccc
     8281 accagcacca tatcgctgcc gctgtctttg taacgctcag taatctgacg acccagttcg
     8341 gcgatacgcg ctttaatctc cgcttcgggg atcattactt ctacagtatg tttcatatct
     8401 ataaccatat gatttaaaaa caaatcactt cgcacgggca ggtttcagca ccgcaacaaa
     8461 gcgcaagaca cgcagtatac cagtaaaact attcattcgc ggaatctgtt aataaagctc
     8521 gaattgtgat gacgatcaca catgttaaaa cctataatta attgctacta aaatattaat
     8581 gaattgaaat ggtgtctatt tatggcaatt aacaataccg gctcgcggcg attactcgtc
     8641 acgctaacag ccctttttgc agcgctttgc gggctgtatc tactcattgg cggaggctgg
     8701 ctggtcgcga ttggcggctc ctggtactac cctatcgctg gccttgtgat gctcggcgtc
     8761 gcctggatgc tgtggcgcag taaacgcgcc gcgctttggc tgtacgcagc cctgctgctc
     8821 ggcactatga tttggggcgt ctgggaagtt ggtttcgact tctgggcgct gactccgcgc
     8881 agcgacattc tggtcttctt cggcatctgg ctgatcctgc cgtttgtctg gcgtcgcctg
     8941 gtcattcctg ccagcggcgc agttgccgca ctggtggtcg cactgctgat tagcggtggt
     9001 atcctgacct gggccggatt taacgatccg caggaaatca gcggcacctt aagcgccgat
     9061 accacgccag cagaagccat ctcccccgtt gccgatcagg actggcctgc ctatggtcgc
     9121 aatcaggaag gtcaacgctt ttcaccactg aagcaaatta acgctgataa cgtccacaag
     9181 ctgaaagaag cctgggtgtt ccgtactggc gatgtgaagc agccgaacga tccgggcgaa
     9241 atcaccaatg aagtgacgcc aattaaagtg ggcgaccccc tttacctgtg taccgcgcat
     9301 caacgtctgt ttgcgctgga tgccgccagc ggtaaagaga aatggcatta cgatcctgag
     9361 ctgaaaacca acgagtcctt ccagcatgta acctgccgtg gcgtctctta tcatgaagcc
     9421 aaagcagaaa ctgcttcgcc ggaagtgatg gcggattgcc cgcgtcgtat cattcttccg
     9481 gtcaatgatg gtcgtctgat tgcgattaac gctgaaaacg gcaagctgtg cgaaaccttc
     9541 gccaataaag gcgtgctcaa tctgcaaagc aatatgccgg acaccaaacc gggtctgtat
     9601 gagccgactt cgccgccgat tatcaccgat aaaaccatcg tgatggccgg ttcagtcacc
     9661 gacaacttct caacccgcga aacgtctggc gtgatccgtg gttttgatgt caacaccggg
     9721 gagctgctgt gggcttttga tccgggcgcg aaagatccga atgcgatccc gtctgacgaa
     9781 cataccttta cctttaactc accaaactcg tgggcaccag cggcctatga cacgaagctg
     9841 gatctggtct atctgccgat gggcgtgacc acgccagata tctggggcgg taatcgcaca
     9901 ccggaacagg aacgttatgc cagctcgatt ctggcgctga atgccactac cgggaaactg
     9961 gcgtggagct accagaccgt tcaccacgac ctgtgggata tggatcttcc ggcacagccg
    10021 acgctggcgg acatcaccgt taatggtcag aaagtgccgg tcatttatgc tccggcgaaa
    10081 accggcaaca tttttgtgct cgatcgtcgc aatggtgaac tggtggttcc tgccccggaa
    10141 aaaccggttc cccaaggtgc tgctaaaggc gattacgtga cccctactca acccttctcg
    10201 gaactgagct tccgtccgac gaaagatttg agcggtgcgg atatgtgggg agccaccatg
    10261 tttgaccagt tggtgtgccg cgtgatgttc caccagatgc gctatgaagg cattttcacg
    10321 ccgccatctg aacagggtac gctggtcttc ccgggtaacc tggggatgtt cgaatggggc
    10381 gggatttccg ttgatccgaa tcgtgaagtg gcgattgcca acccaatggc actgccgttt
    10441 gtttcgaaac tgatcccacg cggtcctggt aacccgatgg aacagccgaa agatgccaaa
    10501 ggcacgggta cggaatccgg catccagccg cagtacggtg taccgtatgg cgtcacgctc
    10561 aacccgttcc tctcaccgtt tggtctgcca tgtaaacagc cagcatgggg ctatatctcg
    10621 gcattggatc tgaaaaccaa tgaagtggtg tggaagaaac gtattggtac gccgcaggac
    10681 agtatgccat tcccgatgcc tgtaccggtg ccgttcaata tgggtatgcc gatgcttggc
    10741 gggccaatct ccacggcggg taacgtgctg tttatcgccg ctacggcaga taactacctg
    10801 cgcgcttaca acatgagcaa cggtgaaaaa ctgtggcagg gtcgtttacc agcgggtggt
    10861 caggctacgc caatgaccta tgaagtgaat ggcaagcagt atgtggtgat ctccgcaggc
    10921 ggtcacggtt catttggtac gaagatgggc gactatattg tggcttatgc gctgccggat
    10981 gatgtgaagt aaccataatg cccggagaga tccgggcata tttccgaata cggtcttttt
    11041 ataccgtaaa ccctaacatc atccccgtat cttcatgctc cagcagatgg cagtgcgcca
    11101 tataagcatg ttctttcggt gcatcgtgat taaacttcac cagcacttcg ctgacattac
    11161 cttctacctt aacggtatct ttccagcctg cgcgatgcgt cgctggcggt ttgccatttt
    11221 ctgacaagat acggaactgc gtaccgtgga tatggaacgg atgcagcatc atgtcgccta
    11281 cgccagagat aacccaacgt tcgtactgcc ctttcgccac cgcaaacatc ggcttattca
    11341 tatcaaacgc ctgaccgttg attttgttgg catggtggaa atcaaacttc ccgctgtggt
    11401 tcatatgatt catattgccg tgccccatat ggcccatcat ctggctgtga tccatcccgg
    11461 ccatcgcctg atcgccatat ttctccatca gcatctgcat ccccatcata tcgagcattg
    11521 ggtccataga aagttgcagc ttgcgtaccg tcagcccttc cagcgaaggt aacgcaggca
    11581 ggctacttaa tgtgtctggc aaagcaccag aggcactaat ggcaatcggc tgaatccgca
    11641 ttaccggatg cggcttgtca aacggcgcaa tcgccatccc catctggctg accggcagcg
    11701 tcaccaggtc aaagggtttg ttgtcgttaa cctccaccag cacttcaaaa cgctcgccca
    11761 tcagcactgg cagctcgttc accttcaccg gtacaggtag cagaccgccg tcgctggcaa
    11821 tcacataaag cgggcgattg tcgctggtgg cgaaattgag cgagcgggca ttacagccat
    11881 tgagcaaacg caggcgcagc cagccacgcg gggcagcgtg ttgcgggtag attacaccgt
    11941 tggtcagcaa cgtatcgcca aaccaaccca cagcggcggt catcacatcc agttgataat
    12001 caatctgccc gtcagcgtta aattttttat cctgaacaat caccgggaca tcatcaatac
    12061 cccactgttt tggcagcatt aatttcagga tctcgtcatc ttcaatcacc accaaaccag
    12121 ccagccccat cgccacctga cgtccggtct tgccatgttg atgtggatgg aaccagcagg
    12181 tagcggcagg ttgatcaacg ttcaacgtca ccgagcgctt gccacctggc ggaataattc
    12241 cctgcgggcc gccgtccact tcacccggta cttccagccc gtgccagtgc aacgtcgtct
    12301 cttccgtcag ttggttgtag atatcaaccg ttaccgcttt gccacgctgt aatttcaccg
    12361 ccggccccag cagattgccg ttatagcccc aggtagttgc ggttttcccg ccaaaggtgg
    12421 actgacctgc gccaatagtt aactgaatgc gattacgggc atcggtcgtg agcaaatcag
    12481 ggattggtaa cgttgggcgt tccgccgcaa atactgcgcg gctccacagc ggcaaggctg
    12541 aagccacacc cagcgcgacg gaatatttca agaaatcacg acgttgcata gttatttcct
    12601 tattcttaag caggcgaaac aaaatcaaac gttgagcata gtccttcccc ttacgggaag
    12661 gtcaagccgc agacacaatt taatcgacaa taatggtcgt cgcctcgctc gcagtgtgct
    12721 aacgtttatc ttctttaagc ccctggtaga agcaatgaag acgtttttca gaacagtgtt
    12781 attcggcagc ctgatggccg tttgcgcaaa cagttacgcg ctcagcgagt ctgaagccga
    12841 agatatggcc gatttaacgg cagtttttgt ttttctgaag aacgattgtg gttaccagaa
    12901 cttacctaac gggcaaattc gtcgcgcgct ggtctttttc gcccagcaaa accagtggga
    12961 tctcagtaat tacgacacct tcgacatgaa agccctcggt gaagacagct accgcgatct
    13021 cagcggcatt ggcattcccg tcgctaaaaa gtgcaaagcc ctggctcgcg attccttaag
    13081 cctgcttgcc tacgtcaaat aatccctgat acctttttgt agaaatattg accgtgcatc
    13141 cgcggtcaat gttagctatt atgttgcgcc atttttttac gggtgttaac aaaggaggta
    13201 tcaacccatg gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt ttgggcagta
    13261 ctttgcggta gataacgttc tgtatcatga aaagaccgat caccaggatc tgatcatttt
    13321 tgagaacgcc gcatttggtc gcgtaatggc gctggatggt gtagtacaaa ccaccgagcg
    13381 tgacgagttt atctatcatg agatgatgac ccatgttccg ctactggccc acggtcacgc
    13441 gaaacatgtg ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag taacccgaca
    13501 taaaaacgtt gagtcaatca cgatggtgga aatcgatgcg ggtgtcgtgt cgttctgccg
    13561 tcagtatctt cccaaccata acgccggtag ttacgacgat ccgcgcttta aactggtgat
    13621 cgacgatggt gtcaatttcg ttaatcaaac caaccagacc tttgatgtca ttatctccga
    13681 ctgcaccgat cctatcggtc ccggcgaaag tctgtttact tctgcatttt atgaaggctg
    13741 caaacgttgc ctgaatcctg gcggtatctt cgtcgcacaa aacggcgtct gctttttaca
    13801 gcaggaagag gccatcgaca gtcatcgcaa actcagccat tacttcagcg atgttggctt
    13861 ttatcaggcg gcgatcccga cctattacgg cggcatcatg acctttgcat gggcgacaga
    13921 taacgacgcc ttacgccatc tctcaaccga aattattcag gcgcgttttc tcgcttctgg
    13981 cctgaaatgc cgttattaca atccggcagt ccatacggca gcttttgcct taccccagta
    14041 tctgcaagat gcactggctt cacagccgtc ctaaggagaa gataagaaat tgaaaaaact
    14101 gaaactgcat ggctttaata atctgaccaa aagtctgagt ttttgtattt acgatatctg
    14161 ctacgccaaa actaccgaag agcgcgacgg ttatattgct tatatcgatg aactctataa
    14221 tgccaaccgt ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg ccaatattct
    14281 taacatcgcc cgccaggatt acgaaccaca gggtgccagc gtcactattc tggtgagtga
    14341 agaaccggtt gacccgaaac tcatcgacaa aacagaacac cccggcccac tgccagaaac
    14401 ggtcgttgcc catctcgata aaagtcatat ttgcgtacat acctacccgg aaagtcatcc
    14461 tgaaggcggt ttatgtacct tccgcgccga tattgaagtc tctacctgcg gcgtgatttc
    14521 tccgctgaag gcgctgaatt acctgatcca ccagcttgag tccgatattg tgactattga
    14581 ttatcgcgtg cgcggtttta cccgcgacat taacggtatg aagcacttta tcgaccatga
    14641 gattaattcg attcagaact ttatgtccga agatatgaag gcgctgtatg acatggtgga
    14701 tgtaaacgtc tatcaggaaa atatcttcca taccaagatg ttgcttaaag agttcgacct
    14761 taagcactac atgttccaca ccaaaccgga agatttaacc gacagcgagc gccaggaaat
    14821 taccgctgcg ctgtggaaag aaatgcgcga gatttattac gggcgcaata tgcccgctgt
    14881 ttaacgtctc aggcggagca cggaggctcc gcctgattta cttacttctg ctgcacgaaa
    14941 ttacggtaag ccgccaccac ctgaagaaaa tcctcaacgc cgcacagcga caagctctct
    15001 tcatcgtagt agttcatccc ctcttccatt tcatcgccag cgaattccag ttgattggcg
    15061 cgaaccatca cctcttcacc gtccatccac agagtgtatt catgccctgc ccgctgccag
    15121 gatcgttcgc tacctttcaa tgcgtgcgcc gcttgttcca cttcatcaag caaggccagg
    15181 ttttctttca cctcttcatt aaaccagtgc ccgaccactt catgccccat ggacatacgc
    15241 acctttacca ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg ttcctctgct
    15301 cccggcaatg cgccgaaatc gcattgcgcc attgatgtaa ttatcgcagc cttaccggag
    15361 aaaaacagcg gaacggcgat ggtttagaaa taaaaaatgc ccgggaatct cattcaccgg
    15421 gcattgtgtc gtttatgcgc agcgcgtgcg ctgacttttt aaaccgcagt ctggaaaatc
    15481 accccatcgg ctttttcggt gtactgagaa agctggttga agttcagata acggtaagta
    15541 tcaacggcag ttttatctac ttgcgccacg taggtctggt actcttccgg cgtcggcagt
    15601 ttgccaatca gcgccgcaac agcagccagt tccgcagaag ccaggaagac attcgcgccc
    15661 gtccccaaac ggttcgggaa gttacgggta gaggtggaaa ccaccgtcgc accgtctgct
    15721 acacgcgcct ggttacccat acacagggaa cagccaggga tctcgatacg agcaccactc
    15781 ttaccgaaga cgctgtagta gccttcttcg gtcagctgtg cagcgtccat acgcgttggc
    15841 ggtgccaccc acaggcgggt cggcaactga cctttgtgcg catccagcag tttacccgca
    15901 gcacggaagt gaccgatgtt agtcatgcaa gaaccgataa acacttcgtc gatcttctcg
    15961 ccctgtaccg cagacagcgg acgagcgtcg tccgggtcgt tcggtgcaca gaggattggc
    16021 tctttaatat ccgccagatc gatgtcgatc actgccgcgt attccgcatc tgcatcggct
    16081 tccagcagct caggattcgc cagccatttt tccatgccct gaatacgacg ttccagggta
    16141 cgacgatcgc cgtaaccttc cgcgatcatc cacttcagca ggacgatgtt agagttcagg
    16201 tattcgatga tcggttcttt gttcagcttg atggtacaac cggcagcaga acgctcggca
    16261 gacgcatcgg tcagctcaaa tgcctgctca actttcagat ccggcagacc ttcaatttcc
    16321 agaatgcgac cagagaagat gtttttcttg cctttcttct caacggtcag cagaccttgt
    16381 ttgattgcat acagcggaat ggcgtgaacc aggtcgcgca gggtgatgcc cggctgcatt
    16441 ttgcctttga agcgcaccag aacggattcc ggcatatcca gcggcattac gcctgttgcg
    16501 gctgcaaatg ccaccagacc agaacccgcc gggaaagaga taccgatcgg gaaacgggta
    16561 tgggagtcac cgcccgtacc gacagtatcc ggcagcagca tacggttcag ccaggagtgg
    16621 attacgccgt cacccggacg cagcgacaca ccgccacggt tcataatgaa gtccggcagc
    16681 gtgtggtgcg tattcacgtc aaccggtttc ggatatgccg cggtgtggca gaaagactgc
    16741 atcaccaggt cagccgagaa gcccaggcac gccaggtctt tcagttcatc acgggtcatc
    16801 gggccggtgg tgtcctggga acctacagaa gtcattttcg gttcacagta cgcgcccgga
    16861 cgaatgcctt tcacaccgca ggcacgacct accattttct gcgccagcga gaagccgcga
    16921 tcgctctcag cgacatcttt cgcctgacgg aacacatcac tatgcggcag accaagtgct
    16981 tcacgcgctt tggtggtcag gccacgcccg ataatcagcg gaatacggcc gccagcacgc
    17041 acttcatcaa tcagcacgtc ggttttcagt tcgaaggttg ccagcagttc gccggtttcg
    17101 tggttacgca cttcaccttt gtacgggtaa acgtcaatca cgtcacccat gttcaggtta
    17161 gagacgtcca cttcgattgg cagtgcaccc gcatcttcca tcgtgttaaa gaagatgggt
    17221 gcaattttac cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg tggaatatca
    17281 tcacccataa accacagtac ggagttcgtg gcggatttac gcgatgaacc cgtacccaca
    17341 acgtcaccga cgtacgccag cgggaaacct ttctgttgca gagcttcaat ctgtttgatc
    17401 ggaccaacaa caccaggctg atctggctca ataccttcac gggcgttttt cagcatcgcc
    17461 agcgcgtgca gtgggatatc cgggcgtgac cacgcatccg gtgccggaga aaggtcatcg
    17521 gtgttggttt cgcctgtgac tttgaagacg gtaacggtca gtttttcagc cagtgccggg
    17581 cgattcagga accattcggc atctgcccag gactgcatta cctgcttcgc atattcgtta
    17641 cctgctttcg ctttctcttc tacgtcatag aagttatcga acatcagcag cgtgtgagaa
    17701 agtgctttgg cagcgatcgg tgccagtttg gcatcatcca gcgcgtcgat cagcggatga
    17761 atgttgtaac caccctgcat ggtgcccagc agttcgatgg ctttttccgg agtcagcagt
    17821 ggggatttgg cttcgccttt cgcgacagca gccaggaagc ctgctttgac ataggcggct
    17881 tcatcgacgc cagggggaac acggttggtt aacagatcta acaggaattc ttcttcgccc
    17941 gcgggcgggt ttttcagcag ctctacaagt gcggccattt ggtttgcatc caggggtttg
    18001 ggcgcaatcc cctcagcggc acgctcagct acgtgcttac ggtattcttc tagcacgacg
    18061 gttctcctcg ctctcattgt catagtgcgg caggcgattc tcttcacgct cctgtgagac
    18121 agcagtttgt agggtaaatg cccagtaccg caacgggcag aatagcagga ttttgacgat
    18181 gtgttaatct gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta aggatggtct
    18241 agagaatgtt tccctccgta aaaacttcgt taattcgcat ggtgataatc acctttctca
    18301 acgaatccca agattagtca aaatttaaac tacggcctct ttatactcgg attcacagca
    18361 cctgcgggtg gcagttcgcc gaccattgcg atttccttga gatccgaatt atgaaaatga
    18421 ctttgccgtt taaaccccat gtgctggcgc taatttgcag tgccgggctt tgtgccgcct
    18481 ctaccgggct atatataaaa agccgcacag tggaagcgcc tgtggaaccg caatcgacac
    18541 aactggctgc gcctgacatc tccgcagtta cgcttcctgc aacggtttcc gtgcctcctg
    18601 taacacctgc cgtcgtcaaa tccgcattca gcactgcgca aatagatcaa tgggttgcac
    18661 ctgtcgcgct ataccccgac tctctgcttt cacaagtgtt aatggcatca acctatccgg
    18721 caaacgttgc tcaagcagtg caatggtcgc acgataatcc acttaaacaa ggcgatgctg
    18781 ctattcaggc ggtatctgac cagccctggg acgccagcgt caaatcactg gtggcctttc
    18841 cacagttgat ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt
    18901 ttctggcgca gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac
    18961 aaaccggctc gctgaagtca tcaactgaac aaaagatcat cacgacaacg aaaaaagtcg
    19021 taccggtaaa tcagcccgcc aacgcccctg ccacacaatc aaataccgtt tctacgtcca
    19081 gccccgtcgt cgcagaacct gcaccgaccg taataaccat tgagccagcc aatccggatg
    19141 tggtttatat tccgaactac aacccaaacg tggtttacgg cagttgggcc aataccgctt
    19201 atccgccagt ttatctgccg ccgccagccg gagaaccgtt tgttgacagc tttgtgcgcg
    19261 gattcggcta tagcatgggc gttgctacca catacgcact attcagcagc atcgactggg
    19321 acgacgacga tcatgaccat catcatcatg acgatgatga ttatcatcac cacgatggcg
    19381 gtcatcgtga cggtaatgac tggcaacaca acggcgacaa catcaatatc gacgtcaaca
    19441 atttcaaccg tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc
    19501 caaactaccg taatggtgtg acctatcatg atcaggatat ggcaaagcgg tttcatcaaa
    19561 ccgatgtcaa cggcggaatg agcgccacgc agttacctgc tccaacacgc gacagccagc
    19621 gtcaggcggc agcaagtcag tttcagcaac gaacacacac cgcccccgtc attacgcgag
    19681 atacccaacg tcaggcagcg gcacagcggt ttaatgaagc tgaacacaat gggagctatg
    19741 acgacttccg cgacttcagc cgtcgccagc cactgaccca gcaacaaaag gacgccgctc
    19801 gtcagcgtta tcagtcggct tctcctgagc agcgccaggc ggctcgcgag aaaatgcaga
    19861 ctaacccaca gaaccagcag cgaagagagg cggcacgtca gcgtattcag tccgcgtcgc
    19921 ctgagcagcg ccaggcggtt cgcgagaaaa tgcagactaa cccgcagaat caacagcaaa
    19981 gagatgcagc acgtcagcgt atccagtctg cctcgcctga gcagcgtcag gtttttcgag
    20041 agaaagttca ggagagccgc ccgcagcgtc taaacgacag taaccatact gcccgattga
    20101 ataatgagca acggtcagcg gtacgcgaac ggttgtctga gcgtggagca aggagactgg
    20161 aaaggtaaat tgcaggcgta aaaaagcggc gttgttagcc gcttaagact gatgacaaac
    20221 atcgaatcgc ccgatgcgca agcttatcgg gcctacacaa gccatgcaat atattgaatt
    20281 tgcatgattt tgtaggtcgg ataaggcgtt tacgccgcat ccggcatcaa ccacgagcac
    20341 tttatcaaca atctgaagcg gccagaatag ccgcttctta atttgccgga tgttccggca
    20401 aacgaaaaat tacttcttct tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa
    20461 cacttctgcc gccaggccca cagactcgtg cagagtcggg tgcgcatgga tggtcagtgc
    20521 gatgtcttca gcgtcacaac ccatttcgat tgccaggccg atttcaccca gcagctcacc
    20581 gccgttagta ccgacaattg caccaccgat cacacggtga gattctttgt cgaaaatcag
    20641 cttggtcata ccgtctgcgc agtcggaagc gatagcacga ccagaagcag cccacgggaa
    20701 ggtggcggtt tcatagctga tgcctttctc tttcgcttct ttctcagtca gaccaaccca
    20761 tgcaacttct ggttcggtat aggcgatgga cgggataact ttcggatcga agtagtgttt
    20821 cttaccggcg ataacttcag cggcaacgtg accttcgtga acacctttat gtgccagcat
    20881 cggctgaccg acgatatcgc cgatagcaaa gatgtgcggt acgttggtac gcagctgttt
    20941 gtcaacgcgg atgaaaccac ggtcgtcaac ttccacgcca gctttgcctg cgtcgaggtt
    21001 tttaccgttc ggcacacgac caattgctac cagcacggcg tcgtaacgct gcggttcagc
    21061 tggtgctttt ttgccttcca tcgtcacata aatgccgtct tctttcgctt caacggcggt
    21121 aactttggtt tccagcatca ggttgaattt tttgctgata cgcttggtga agactttaac
    21181 gatgtctttg tcagctgccg ggataacctg gtcgaacatt tcaaccacat caatctgtga
    21241 acccagcgcg tggtatacgg tgcccatttc cagaccgatg ataccgccac ccattaccag
    21301 caggcgttct ggtacttctt tcagttccag cgcgtcagtg gagtcccaga tacgcggatc
    21361 ttcatgcgga ataaacggca gttgaatcgg gcgagaaccc gctgcaatga tcgcgttgtc
    21421 gaagttgatc acggttttac cgttctcacc ttcaacttcc agggtgttag ccccggtaaa
    21481 tttacccaga ccgttgacca ctttgacttt gcggcctttc gccataccag ccagaccacc
    21541 ggtcagctga ttgatcactt tctctttcca ggtacgaatc ttgtcgatat cggttttcgg
    21601 ttcgccgaag acgataccgt gttcagccag cgctttggct tcttcgatga cttttgctac
    21661 gtgcagcagt gctttagaag ggatacagcc gacgttcagg caaacaccgc caagggtgtt
    21721 gtaacgttct acgattacgg tttccagacc taaatcagcg caacggaagg cagcggagta
    21781 acctgcgggg cctgccccaa gtaccacgac ctgagttttg atttcagtac tcatcatgac
    21841 ctctatatat ttatctccgg cggtcatacc cgtcgtcttt caggccgcag cggcgttagc
    21901 ttcacgccct ccccccagtc acttacttat gtaagctcct ggggattctg gcgcttgcta
    21961 ccttgctgca cccagaaatc catagggtat gtgaagtaat accctaacca ccaccgggtc
    22021 gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa acggcaaccg
    22081 atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa aagccggccg
    22141 ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg tgttgttaat
    22201 gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga
    22261 aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga
    22321 cttggaaacg ccaaggatag ccacttccgg cgcgttcaca atcggcgcga agtgggtagt
    22381 acccaggccg ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag
    22441 cttaccgtca cgcgctttct tggaaatagt catcagctcg cgagacaact cgatgatgcc
    22501 tttcttattg acgtctttga ataccggaac aaccagaccg ttcggggtat ccaccgccac
    22561 accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca gcgaactgtt
    22621 aaagcgaggc atctgctcaa gagctgcagc aaccgctttc atgatgaaga caaccggggt
    22681 gatcttcaca tccagcttac gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc
    22741 cagctcggtg atatcggttt tgtcgaagtg agtaacatgc gggatcatca cccagttacg
    22801 gctcaggttc gcaccagaga ttttctggat gcggcccagt tccacttctt cgatttcacc
    22861 aaacttgctg aagtccactt taggccacgg cagcatgcct gggataccgc cgccagttgc
    22921 cgccggagcc gcttctgcac gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg
    22981 caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc caaactcgcg
    23041 tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg tcgttttcag caaattcaga
    23101 tttgccttcc gctttcgcag ccggtgctgc tgccggggct tcagcttttg ctgccggagc
    23161 cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa
    23221 gatcataatt agcgaaccag ttttcacttt atcgccaacg ttgactttca gttccttcac
    23281 gacgcctgca aacggagccg gaacttccat agacgcttta tcgccttcta cggtgatcag
    23341 tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc
    23401 gtcaccgccg atatccggaa cgttaacttc tttcacgcca gccgctggtg caggagccgc
    23461 tgccggagcc gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc
    23521 gaagaccata atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt
    23581 cacggtgcct gcaaacggag ccggaacttc catagaagcc ttgtcgcctt ctacggtgat
    23641 cagcgactgt tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac
    23701 ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gctgcagccg ctggtgctgc
    23761 tgccggagcg gcttctttct tctcttctgc ctgagcaggt gcagcgtcag ctgcaccgtc
    23821 ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt
    23881 gatctcttta acgatacccg cctgcggaga cggaacttcc atagaggctt tgtcgccttc
    23941 tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt
    24001 gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt
    24061 acctcttaag ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg
    24121 tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc
    24181 acgacataag aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga
    24241 ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacctgc
    24301 tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcatcgtt catcacctga
    24361 gcgatgtatg gaacgcgcgg agtttccagc gggtgcagca tgttccagcg ttcacaatcc
    24421 tgaccatcac gcgccagctc tgtgaaggag gtcacgctat aaacgtcaga acctacgccg
    24481 taatctttcg ccaggatctc tgccgcttca cggacgtgac gcaggataga accggagccc
    24541 agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg
    24601 ataccttcct cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg
    24661 atgtagtagt aaacgttctc ttgtttttca ccgtacatac gctccagacc gtcatgcatg
    24721 atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg gatagtcagc
    24781 gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag ggtggtacga
    24841 ccggaagtac cgccgatcag gaagccacgc gcttgctggt cgccagccgc ccagcacaga
    24901 tcgccgatac gctggaagcc gaacatcgag taatagatgt agaacggaat catcggcaga
    24961 ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac accctgcgcc cagctcgttg
    25021 atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg
    25081 tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct
    25141 tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac
    25201 ttgttcttca gcatcacgtt cagggcacga acgaaagcga tagtggtaga gatctctttg
    25261 ctctgctctt ccaacagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg
    25321 aagttcggct gacggcttgg cagataaccg tgcagtttct gacgctgagc gtgcagatag
    25381 gtatgctctt cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca
    25441 gacaccggca cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta
    25501 acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggta
    25561 tgagcaagga ttaccgtcgc tttgcctttg gtttcctgcg ctttcttaaa tgcagcgtag
    25621 attttcttcg gatcgtgacc gccacggttc agggcccaga tctgctcgtc agtccagtct
    25681 gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcacgaac gtacgcacca
    25741 tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag ctggatcagt
    25801 ttaccgctgg tatctttacg cagcagttca tcccaacggc taccccacat cactttgatc
    25861 acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg
    25921 gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaagaccaa gttatccagt
    25981 ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg
    26041 ccgaggaacg cgtaaacggt ttgtttagag gtatctttca ggccacggtg ttccagatat
    26101 ttcaggaatt tagcctggta aatagcacca atcggaccca gacccataga tacggtcggg
    26161 aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga
    26221 acttcctgac ggaagttatc caactgctcc tgagtcagac gaccttccag gaaagcacga
    26281 gcgtaaacgc ccggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg
    26341 ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag
    26401 gacgccatgt ggccgcccag ttcgaggtct tttttcgacg cacgcagaac cgtcatgatg
    26461 gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc
    26521 ggttgttctt caacggggat ggtgttgatg tagttgctga taccggtgcc tgcggctacg
    26581 ttgacaccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca
    26641 acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga
    26701 tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga
    26761 gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg
    26821 tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca
    26881 gaaaaatcac taattctttc gttgctccag acgacgtaga gaacgctcac ggcggctctc
    26941 ttcacggctt ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc
    27001 gcgcgcttct tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact
    27061 caccagcggc agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg
    27121 ggccaacatc ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc
    27181 ggtgacggca atctgatact ggagtacggc atttgattcc gcgtccagat cgccagactg
    27241 ctgcgccagc tctatggcat ggtggagttc acggatgcgt tccttgtctt catcggtact
    27301 acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc
    27361 atactgtgat tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca
    27421 taggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc
    27481 gagacgttga atcgcctcac gcaaggaggg acgggagacg tcgaactgtt ttgccagttc
    27541 gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag
    27601 ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc
    27661 ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa
    27721 agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca
    27781 cattttaaca acttgacata tataacgttt caaagttgta actatgcaca aatgttaaat
    27841 ttacccatca aggggtgaga ttaacagaat tgaaacgaaa aaatatgtcc tctgaaccga
    27901 ttcaccaagc aacaaaacgc ataacaattc aattatgtga attaatatgt aaaatgaatg
    27961 ttcagggaag gagagaaagg tagtatttac ctatgttttc tttttacatt gcttattatc
    28021 actgcaatcg ccaccgtaac cttctcgtag cgaaaatcat cacttaacca gaacgtgatg
    28081 attttcaggt gacagattct gtttagaatt cattctgaat ctttgaacaa agacaatcct
    28141 tgcgaacgcc gccattcttt gacgattttc aggatgtttt caggtttacc a
//
LOCUS       DAZFRG010000037        28170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFRG010000037 DAZFRG010000000
VERSION     DAZFRG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.037"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..1153
                     /locus_tag="ACHKUC_001253"
     CDS             32..1153
                     /locus_tag="ACHKUC_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6617539.1"
                     /translation="MGDLGGIQSDLAQLVRLVITEQYDDVRLYVARLVRKYRETMPAL
                     SEQLDLYLRNKPQKPAQSLRKVTAPKQALPQTMPVDEESRLTLLKAPNEKIVSKPLLS
                     SQIEDSLDQIILERKHIDRLKTLGLQPTRSAIFVGPPGVGKTLTASWLAQKLGVPFYV
                     LDLTAVMSSYLGKSGNNLRAALDFAKKGPCVLLLDEIDSIAKKRSDDSDVGELKRLVT
                     VILQEVDEWPSSSLLLAATNFAELIDPALWRRFDLVLNFEKPDSESIKEAIKRFSGPD
                     YAIFARWIDLLAIMFKNESFSNIERSINKFRRSVALGISSDEELIGSFIKDGLSELDR
                     NERKEIAVTLKRNSKLSQHAISDLTGVSRDTIRKYSQKM"
     gene            1177..3402
                     /locus_tag="ACHKUC_001254"
     CDS             1177..3402
                     /locus_tag="ACHKUC_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFO6617540.1"
                     /translation="MAKTNFLIGRGELLTSDIPGPKRVPGKAEVYSLYESKQRLLPQI
                     ASTIENFDDLPSQACPYDYAVAKLTLNPSYIARSFFPAKMLKSTGLTSIGSRTVKITP
                     QSWKRKVTVSEVTTTQMFVAGKRESFKELPHWVSGLKEFSDEAIDFARIERFDAYLPE
                     ERIITIGEQDSSYFEVGVHLLPDNEQDLVQQQFVQFASNLNIKVHTSLAFTAGTLWFV
                     PIQADRITISKLAKFTFVRVIRPVPRLRGIRPIQRNYGPLAQCKLPAEGPVSSEIKVA
                     ILDGGLPSEHAISPWLKSYRVLDDHAEDDPDGLEHGLAVSSAFLFGPISNKSEAKRPY
                     SYIDNLRVLDNKTCQEDPLELYRTLGFIEEVLLSRQYQFINLSLGPDLPIEDTEVHAW
                     TSVIDDLLSDGETLMTVAVGNNGEMDRKSGNARIQVPADCVNALAVGACDTVNDTEWK
                     RAPYSAIGPGRSPGVMKPDLVAFGGDTDEYFHVLGKGKKPVIIPQLGTSFASPYALRA
                     AVGVRAILGNDLSLLAIKALLIHASKQLDHPCTEVGWGKLPEDLNDIIVSPDGVARIV
                     YQGELKPGKYLRAPLPIPEGGLKGRINLAATFCYATTTDPQDPASYTKAGLEVTFRPN
                     DSKIKDGKQNAETKGFFELAKYSTESERRSDMGKWETVLHNSKNMLGSTLKNPVFDIH
                     YNAREAGASIASHKAEKIKYALIITIKASKHQDLYNEILRTYNQVLVPIQPQTSIPIR
                     S"
     gene            complement(3895..4941)
                     /locus_tag="ACHKUC_001255"
     CDS             complement(3895..4941)
                     /locus_tag="ACHKUC_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6617541.1"
                     /translation="MSRKNKKVRMSTRIELADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFEFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMGSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(4941..6692)
                     /locus_tag="ACHKUC_001256"
     CDS             complement(4941..6692)
                     /locus_tag="ACHKUC_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO6617542.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            6847..7683
                     /locus_tag="ACHKUC_001257"
     CDS             6847..7683
                     /locus_tag="ACHKUC_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6617543.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSVQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            7707..8759
                     /locus_tag="ACHKUC_001258"
     CDS             7707..8759
                     /locus_tag="ACHKUC_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6617544.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            8805..9605
                     /gene="gpM"
                     /locus_tag="ACHKUC_001259"
     CDS             8805..9605
                     /gene="gpM"
                     /locus_tag="ACHKUC_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO6617545.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDILREI
                     VTDQDMPDEVRAKLCKACAFARRGLTDADNMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            9708..10202
                     /locus_tag="ACHKUC_001260"
     CDS             9708..10202
                     /locus_tag="ACHKUC_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6617546.1"
                     /translation="MSMVARTEPRPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            10202..10402
                     /locus_tag="ACHKUC_001261"
     CDS             10202..10402
                     /locus_tag="ACHKUC_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6617547.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGA"
     gene            10405..10728
                     /locus_tag="ACHKUC_001262"
     CDS             10405..10728
                     /locus_tag="ACHKUC_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6617548.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            10725..11117
                     /locus_tag="ACHKUC_001263"
     CDS             10725..11117
                     /locus_tag="ACHKUC_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO6617549.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            11114..11521
                     /locus_tag="ACHKUC_001264"
     CDS             11114..11521
                     /locus_tag="ACHKUC_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO6617550.1"
                     /translation="MKLWPTLGVAFLLIAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            11659..12126
                     /locus_tag="ACHKUC_001265"
     CDS             11659..12126
                     /locus_tag="ACHKUC_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6617551.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            12119..12754
                     /locus_tag="ACHKUC_001266"
     CDS             12119..12754
                     /locus_tag="ACHKUC_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6617552.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGAAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            12766..13332
                     /locus_tag="ACHKUC_001267"
     CDS             12766..13332
                     /locus_tag="ACHKUC_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6617553.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DAAAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGTMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            13329..13679
                     /locus_tag="ACHKUC_001268"
     CDS             13329..13679
                     /locus_tag="ACHKUC_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6617554.1"
                     /translation="MTTRYTGMNQDGTGNLNDMGHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            13683..14579
                     /locus_tag="ACHKUC_001269"
     CDS             13683..14579
                     /locus_tag="ACHKUC_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6617555.1"
                     /translation="MPAVDLSQLPDPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            14572..15102
                     /locus_tag="ACHKUC_001270"
     CDS             14572..15102
                     /locus_tag="ACHKUC_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6617556.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPAHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            15105..17255
                     /locus_tag="ACHKUC_001271"
     CDS             15105..17255
                     /locus_tag="ACHKUC_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6617557.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQIANTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAVQKNGDTLSGGL
                     TFENDSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEYFKWRSRQSTTTKDL
                     MTLKWDALNILVNAVINGSLGVGTTNALGGSSIVLGDNDTGFKQNGDGILDVYANSRR
                     VFRFQNGVAIAFKNIQAGDSKKFSLSSSNTSTKNATFNLWGASTRPVVAELGDEAGWH
                     FYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYVKDVRLGTRVVQLMARGGRYEKAGHAI
                     TGLRIIGEVDGDDEAIFRPIQKYINGTWYNVAQV"
     gene            17259..17786
                     /locus_tag="ACHKUC_001272"
     CDS             17259..17786
                     /locus_tag="ACHKUC_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6617558.1"
                     /translation="MQHLKNIKSGNPKTKEQYQLTKNFDVIWLWSEDGKNWYEEVNNF
                     QDDTIKIVYDENNIIVAITKDASTLNPEGFSVVEIPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMATDEERTRLESWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(17815..18348)
                     /locus_tag="ACHKUC_001273"
     CDS             complement(17815..18348)
                     /locus_tag="ACHKUC_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6617559.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(18351..18848)
                     /locus_tag="ACHKUC_001274"
                     /pseudo
     CDS             complement(18351..18848)
                     /locus_tag="ACHKUC_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            19127..19726
                     /locus_tag="ACHKUC_001275"
     CDS             19127..19726
                     /locus_tag="ACHKUC_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6617560.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPVGDIQAEETTRQTQENENR"
     gene            complement(19753..20241)
                     /locus_tag="ACHKUC_001276"
     CDS             complement(19753..20241)
                     /locus_tag="ACHKUC_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6617561.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(20254..23061)
                     /locus_tag="ACHKUC_001277"
     CDS             complement(20254..23061)
                     /locus_tag="ACHKUC_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6617562.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLANNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEADRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATIGSLSLVIGVLYGKLATLRLGFDILTRS
                     MNVVRVLPALWGMLTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRTRAGRGSFYDKD"
     gene            complement(23048..23203)
                     /locus_tag="ACHKUC_001278"
     CDS             complement(23048..23203)
                     /locus_tag="ACHKUC_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6617563.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(23212..23577)
                     /locus_tag="ACHKUC_001279"
     CDS             complement(23212..23577)
                     /locus_tag="ACHKUC_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6617564.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            complement(23632..24144)
                     /locus_tag="ACHKUC_001280"
     CDS             complement(23632..24144)
                     /locus_tag="ACHKUC_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6617565.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(24144..25328)
                     /locus_tag="ACHKUC_001281"
     CDS             complement(24144..25328)
                     /locus_tag="ACHKUC_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6617566.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIVDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            25486..26595
                     /locus_tag="ACHKUC_001282"
     CDS             25486..26595
                     /locus_tag="ACHKUC_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6617567.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            26638..26898
                     /locus_tag="ACHKUC_001283"
     CDS             26638..26898
                     /locus_tag="ACHKUC_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6617568.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLSQN"
     gene            27089..27229
                     /locus_tag="ACHKUC_001284"
     CDS             27089..27229
                     /locus_tag="ACHKUC_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6617569.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(27419..27700)
                     /locus_tag="ACHKUC_001285"
     CDS             complement(27419..27700)
                     /locus_tag="ACHKUC_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617570.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRTGGYDVVCYESDVTDEMIRQWKQENAE"
BASE COUNT         7156 a         7386 c         7211 g         6417 t
ORIGIN      
        1 attcacttaa aatcaagagg ttacagttaa gatgggcgac ttaggtggta ttcagtcaga
       61 tttagcccaa ctagttagac tggtaataac tgaacagtat gatgacgttc ggttatatgt
      121 cgctcgcttg gtgcgtaaat atcgggaaac tatgcctgct ctatccgagc agcttgatct
      181 ttatctacga aataaaccgc aaaagcctgc acaaagcctg cgtaaggtta ccgcaccaaa
      241 acaagcgctg cctcaaacca tgcctgtgga cgaggagtca aggcttacct tgcttaaagc
      301 accaaatgaa aagattgtga gcaaacctct actgtccagt cagatagaag actcactgga
      361 tcaaattatt cttgagcgga agcacatcga tcgcctcaag actctcggct tacaaccaac
      421 acgctctgcc atttttgttg gccctccggg tgtaggtaaa acacttacgg caagctggtt
      481 agctcagaaa ttaggggttc ctttttacgt tcttgattta acggcagtaa tgagcagcta
      541 tctgggcaaa agcggaaata accttcgggc agctttagat tttgcgaaga aagggccgtg
      601 cgttcttctg ttagatgaaa ttgattcaat cgccaaaaag agaagtgatg attctgatgt
      661 tggagaatta aaaagacttg ttaccgtgat actccaggag gttgacgaat ggccgtcttc
      721 tagtttgcta cttgctgcaa ctaacttcgc tgagttgatt gatccagcac tatggagacg
      781 ttttgattta gtgctcaatt ttgagaagcc agatagcgaa agtattaaag aagcaattaa
      841 acgcttttca ggtcccgact acgcgatttt tgcacgttgg atagatctac tggctattat
      901 gttcaaaaat gaatctttta gtaatattga gcgttctatc aacaaattcc gacgttcggt
      961 cgctttaggt atttcctcag atgaagagtt gattgggtcg ttcattaaag atggtttatc
     1021 agaattagac aggaatgaac gtaaagaaat tgcagttact ctaaaaagaa attctaaatt
     1081 atcccagcat gccatatcag acttaaccgg agtcagtagg gatacaatta gaaaatacag
     1141 tcaaaaaatg taatacagca tagataagga gtatgtatgg cgaaaactaa ttttctgatt
     1201 ggacgcggcg aattattaac cagcgatatt cctggtccta aaagagtgcc cggaaaagct
     1261 gaggtttatt ctctttacga atcgaaacaa cgtttattgc cgcaaattgc atctactata
     1321 gaaaattttg atgatttacc cagtcaggca tgtccttatg attatgctgt ggctaagttg
     1381 acgttgaatc cgagctatat tgcacgctct ttttttcctg caaaaatgct taagtctacc
     1441 gggttaacgt ctattggcag caggacggta aaaattacgc ctcaaagctg gaagcgaaaa
     1501 gttactgtgt cagaagtaac tacgacacaa atgtttgttg ctggtaaaag agaatccttc
     1561 aaagagttac ctcattgggt gtcaggactc aaagagtttt ctgacgaagc gattgatttt
     1621 gcacgcatag aacggttcga tgcctattta cccgaagagc gtattattac cattggtgag
     1681 caggattctt cgtattttga ggttggtgtt catttactgc cagataatga acaagacttg
     1741 gttcagcaac aatttgttca gtttgcatca aatttgaaca tcaaagtaca tacttcatta
     1801 gcttttaccg ctggtacatt atggttcgtg ccaatccagg ccgatagaat tactattagt
     1861 aagcttgcga aatttacatt tgtacgagta ataagaccgg ttccaagatt gcgtgggatt
     1921 agaccaatac aaaggaacta cggtccttta gctcagtgta aattgcctgc tgagggcccc
     1981 gtatcttcag aaataaaagt tgcgattctt gatggcggtt taccatcaga gcacgctata
     2041 tcgccatggt taaaatccta tagagttttg gacgatcatg cagaggatga tccagacggt
     2101 ttagagcatg gtttagctgt gtcttcagcc tttttatttg gaccaataag taataaatcg
     2161 gaagcgaaaa gaccatactc ttatattgat aacttaagag ttttggacaa caagacctgc
     2221 caagaagatc cgctggaact atatcgaact ttgggcttta ttgaagaggt attgttgtct
     2281 cgccagtatc agtttatcaa tctaagttta ggtccagacc taccaattga agatacggaa
     2341 gttcatgcat ggacttccgt tattgatgat ttgttaagtg atggtgaaac attaatgact
     2401 gttgctgttg gcaacaatgg agaaatggat cgcaagtctg gtaatgcaag aattcaagta
     2461 ccagccgatt gcgttaatgc tcttgctgtt ggtgcttgtg atacagttaa tgatacggaa
     2521 tggaaacgag caccatatag cgccattggg ccaggcagaa gtcctggcgt gatgaaaccg
     2581 gatttagttg cgtttggtgg tgacacagac gagtactttc atgtcttagg taaaggtaag
     2641 aagcctgtaa tcatacccca gctaggaaca agctttgcct ctccttacgc cttacgagca
     2701 gctgttggtg ttcgggcaat tttagggaac gatttgagcc tactagcaat caaagctttg
     2761 ttaattcatg cgtcaaaaca gcttgatcat ccttgtacag aagttggctg gggaaaactt
     2821 cctgaagact tgaacgacat tatagtcagc cctgatggtg ttgcccgtat cgtctaccaa
     2881 ggtgaattga aaccaggaaa atacttacgc gctcctcttc caatacctga aggtggcttg
     2941 aaaggtagaa taaacttagc ggcaactttc tgttatgcaa caaccactga tccacaagac
     3001 ccagcttcct atacaaaagc tggtttggaa gtgacattcc ggccaaatga ttcaaaaatc
     3061 aaagacggaa aacaaaatgc tgaaaccaag ggattctttg aactggccaa atattcaact
     3121 gaaagtgagc gacgttctga catgggcaaa tgggagaccg tcttacataa ttcgaagaac
     3181 atgctaggtt caactctaaa aaatcctgta tttgacattc attataacgc tcgtgaggcc
     3241 ggtgcaagca tagccagcca taaggcagaa aaaatcaagt atgctcttat aattaccatt
     3301 aaggcatcaa agcaccaaga cctgtataac gaaattttac gtacttataa tcaggtgctc
     3361 gtgccaattc aaccgcaaac atcaattcct attcgttcgt aatccttcaa gacataatgc
     3421 ccctcaattt gaggggcatt tctttgcaca aaagtgcaca aatttgcaca atttttttga
     3481 acgacttttt gcccttccgg cccgcatggc ggctggatcg gtcaaggatc cgtgcgtgca
     3541 caaaaaaacg cgctttttct gcgcgcaggt gacgggggaa cagcccgcgt ttcagggggt
     3601 aaatagcatt ccctgaacga tgtcgcagag atacaacaga atggctgtat ttctcacgct
     3661 gagcgtgaaa aagacgtgag ggcttttgat ttatggggtg aaaggtaagg ccgtcaaaat
     3721 cgcactgaga cggcgagaac atgcagtcaa cgcggtggga ttgcgtaaga gtctgactgt
     3781 cgatggtggc gataaactgg aaggcgtcgt gaaattatct gactgataca ggagctggag
     3841 agtcggggca taaatttttt atgccccggc gaagcagcag acaagcgaag cgcgtcagga
     3901 tgtgggctgg gtgtctaaca gtgcgtaagg gttaaagcgg atcacctctt cgccaagcca
     3961 gtcattgatg tgcttcatgg cctccatgac aggcatcagc tcgttaattg cgtaaacccg
     4021 cgcggccttc tccacatcac caaacgcact tttttcgccc ggcatcgccc ccatcagttg
     4081 cggcggaacg cggtgcgcag ccagcacatc atcacgggat gccgccttaa cattcatgaa
     4141 ctcatccttt gcggtgatct gctggaacgg caaaatttgc accccctctt tgcccccgtt
     4201 gggagcatgg atgagcacgt ttttaaacgc accaccacca cgcgcaccct gtaacgtttc
     4261 tttcagggag cccatgcttt cgcggtttac ctgcgctgca ccaatgtaga tgatgcaccc
     4321 ggcgtgggag ccgttgtcgt agtacagttt tctgaacatg tccgccgaat gagaaaggct
     4381 ggccgagagt aatgcgccaa gatattccgg catgccgtag atttcctgat taatatccgg
     4441 attcatcaga tggcacactt taccagggcg aaactcaaac gcgtccttgc catcctgcac
     4501 ataccaccat gattcaagat cgcttccgcg tcgcatgtat ttcgccagtg cgtgccgtaa
     4561 tttaagcggt tcgccgagca tattgcttcg aagctcaagg aatgcgttac cgaacacaaa
     4621 ccagtccagc gccagcgccg agaaatcctg ccgggaaagc agcgggtgcg ggatgtagca
     4681 accgagcaat acattgcgct taaagtaaag cgcagactga tgccaggacg tttgccgggc
     4741 ggctcttgcc agaccgtacc agtccaccgg ggtttcgtac caccgcccgt tatcagcaca
     4801 gtacatattg tccagcaggt catgcccggt caggcggtaa ggaccatcaa atgtgaatgc
     4861 actgagcgac gattctttcc tgagcgcatc agcaagctca atgcgtgtac tcatgcgcac
     4921 ttttttattt tttctgctca tcagaactcc ataaccgtga aacgctcgtt ttctccttcg
     4981 ccgccaatcg gttcgttaat gacagcaagc atggttgccc acgcaaggtc gccgtggctg
     5041 atccccctcg cgcggtccgt ttcgtaagtg ataaagccgc ccggtgtttt caccttacgc
     5101 acggcgttaa aggctgcgac cagctcgcgt tcagcgcgat cgtattccca ccgcccggcg
     5161 cgcattattt gcagcatttt cagtaccagc gaccgttttg atgacagcgt gaaggtgtac
     5221 ggaatagcag cagggaaaaa ccgtttcact atctgataaa cagcctcccc gttcccgcct
     5281 gtcacatcaa tgccgatgtg ttccacgttg tagcgacacg tgaactcttc aatgactctg
     5341 gcctgttctt caaactccag cccctgaacg cgtcgcgtct ccaccgttcg aaaacggcca
     5401 ccaggaacag ccggaggaac caccacggac acagcgccgc tgtcgccgtt gccactgctg
     5461 ccgtttgcgt catacccaat ccataccgga cgattcccca tcgggcgggg agcaaaaggt
     5521 ttccagtcct tccagtcgtc gtatccgtca acaccgcagc caatcaggat attcaggtta
     5581 aatgccgatt ccccttcgcg gacaaactca cacatataga gattgaggaa ctcgtcttcg
     5641 gtgttttcat cacgaatttc gtcaatatcg gtgtgtttcc agccgtgatt aaccacatct
     5701 tccagcgtga caatctgccg ccacgtccgg tcagggcaga taagcccgtt atgcagcgtt
     5761 ttccagtcca cagaaaaacg ctggcgttta tgcgtggcct ttttctcgtt ccagcggtcg
     5821 ccattccagt aggcgtatgc ctcgtgcgtt tcggtggatg gcgtggagaa gtaggtgcgc
     5881 cgcagtccgc tgagggttgc catagcgcca gccaccttgc gcagttcagc aaagcgactg
     5941 acccagaaaa attcatcaaa ataaaaattg cccgtatagg actgcgccga cgcagcagaa
     6001 gtgccgagaa aatgcagctc tgcgccgttg gagaggatga ttttatcgcc ccctttcagc
     6061 tccacatcaa cttcagccgc ggccttctga ataatgcttt taaactggaa cgcctgacga
     6121 cgcgacgcag acaaaaaaat ctggttacgc tggtaaggtt gcgccacatc gtcacgcagc
     6181 gccatcagca gtgcttcctg tgcaaaatac caggtcgccc caatctgtcg ggatttcagg
     6241 atcatcctgt tacgtatccc ggcttccctg caaagggtca gggagtcaaa ccagccccgc
     6301 tgatgccact ccagcctgct gatgattttt tcccgcagtg cggcaatctg ttccggcgtg
     6361 aaatgatttt tgagtttttt cgcccggcct ttctttcctg cggccatcac atccggctgg
     6421 ccatcatgca gctttttaag ctgccgggtc agcaggtcta tttccttaaa gtcaccgcct
     6481 gttttattct gtttttcagt aagctggatg aggcgcgcat cgatggactg cgtgacacgc
     6541 tgcacgggtg gcgtttcatc ccactggtcg cgttttttcc acgcataaat cgtgttcggg
     6601 tttattccca tcagacgtga tatttctgcg ggcggataac cctgccagta aagttgccgc
     6661 gcacgctggc gcacaaaagc gtcctgaatc attgctcccc ctgagtaatt acaggaagat
     6721 tacccgcgcg cgaaaccgtt ctccttaacc ccctgttctg gccgttttct tacaacaaaa
     6781 gccctttgta tcagcctgtt acgctttgcc atcatgactg aagaaccagt cagaggggca
     6841 aaaactatgg ctaatgaaaa aaagacatcc cgcaaaaagt ttcgcgtggc tgtctccgga
     6901 tcaactgttg atggccgtga aatcagtccg gtgcatctgc gtgaagccgc cgagaacttc
     6961 aacccggatg tttacgctgc ccgcgtgaac gttgagcact atctctcgcc atgcccgtca
     7021 agcgaatttt ccgcaatggg cgatgtcacc gcactgagta cggaagacat tacggaaggt
     7081 ccgctggccg gacgtactgc gctgtatgca gaaatcgaac cgaccgagcg catgaagcag
     7141 cttgtcgcgg acggcaagaa aatctattcc agtatcgaac tgcacccgca gttctccgtt
     7201 aacgggcgcg cctatctggt cgggctggcg atgaccgaca ccccggcaag cctgggcact
     7261 gagcgcctga aattcacggc acagcaacgt caggcggtaa tgacgttcaa cagtgtccag
     7321 ggtgaagcgc cgcttatctc tgaagccatc gagtctgaaa tcatcgaaat ggcagaacaa
     7381 cgccaggaag aaggcaccca gtggtttaac cgcgtaatgg ggattattgg ccgtggccgc
     7441 aaagcggatg acgccagttt ttcccgtatt caggaagcgg tggaaggcgt cgcaacgtca
     7501 caggccgaca ttatcgaccg ttttaatgtg ctggaaaccc gccatcagca ggaccgccag
     7561 aaaattacgt cactgaccac agagctgaca gcactgaagg aaaaactgcg cacgcaggac
     7621 ggcgatccgc agaaccggtt caccgcaacg ggcgcagcct ccgaccagct ggctgacttc
     7681 tgataagaca aaggagcaaa ttttttatga atctggtgat gtcagatatt acccgcaaca
     7741 agctgggttg ctatatggcg cagcaggcgt cgcttaacaa tatcccggtt tctgcgctgg
     7801 tatcgcgatt taccgtagaa cccgcggtgc agcagcgttt tgaaaacgcc tcaaaggaaa
     7861 gtaccgaatt tacgaaaaga attaacgtga tcggcgtgac cgaccagaaa ggcgaaaaaa
     7921 tcctcctgga taccacagga ccgattgcgc gcacgaatac cagttatgac ggaacaaaac
     7981 gccgtaaccc gaataacgtg gttgatctga aaaaccgcaa ataccagtgc gaacaggtga
     8041 actacgacac gtttatttcg tatccgcagc ttgatgcctg gtcggcacac cctgattttc
     8101 agtcacgcat cagcgcacag attgcccgac aggtggcgct tgaccgcatc atgatcggtt
     8161 tcaacggcac gtctcacgcg gatgagtcca acttcagcac caacaagctg cttcaggacg
     8221 ttaacgtggg ctggctggag cacatcagaa ccgacgccag cgaacgcgtt atgaatgacg
     8281 tgacgctgac ctcccgcaac atggacaaca ccgtggcgca cgcgggtaag tatgcgaacg
     8341 ctgatgcact ggtacaggac gcgcgttcat ccctgctgga tgaatggcac aaggaagctg
     8401 acgacctcgt ggtgatcatg gggcgcaacc tgtttaactc gctgcgtctg cccgtgctga
     8461 acagcatcag cggccagaat cccaatgcgg aattactcgc cgggcagctc atcctgtcat
     8521 cgcgcaccat tggcgggctg ggcgtgttcc ttgcgccgtt cttcccggat gcaacgatgc
     8581 ttatcacctc gttcaacaac ctgtcgattt actggcagaa aggttcaatg cgtcgcctga
     8641 tgaaagacga gccggaatac aaccgcatcg ccacctacca gtccatcaat gacgcttatg
     8701 tcgttgaaga ctatggcaag tgcgcgatgg tcactggcct gaagttcgcc gacagctaat
     8761 caactcacgg cgggcatcat gcccgcctgt aacggagaga acaaatgatt actcctgcac
     8821 agcaacactg gcagaacgtg atggcacagc gagcaggccg ggcgaatgaa ggcgtggacc
     8881 acgccgcgcg taccgcacat gaagaggtac tgtatcgtct gcgtctggca caggcccggc
     8941 ttaagggcgt acaggccaga agcgcgaaag ccgccatcaa aaaagagttg ttgccggact
     9001 tttccggctg gattgaggga acgctggagg ctgacggcgg acagcaggat gaagtgattg
     9061 ccacgctgat ggtgtgggcg attgactgcg gcgatcttcc gcttgcgctg cgtattggcg
     9121 cgtatgtggt ccgtcataac ctcatcatgc cggataactt tggccgtact gctgccacag
     9181 tgctgaccga agaaatctgc aacccggtgc tgacgcaggc cgggacggat gccgacgcgg
     9241 atttgtccgc ctttatcgaa ccactggaca tcctccggga gattgtcaca gaccaggata
     9301 tgccggacga agtacgcgcc aaattatgca aggcgtgcgc ctttgcccgt cgtggtctga
     9361 ccgatgcaga caacatggcc ttatcactga agctgctgcg cgaagcgatg cacctgaacc
     9421 cgaacgcagg tgtgaaacgc gagattgcaa ccctttcccg cgccctgaaa aaagccgatt
     9481 ccgcagccgc accagaagac gccagcgcac agcaggcgca ggacgaaagc agcaaaagta
     9541 aaaagacaac gcggaagcct gcaacacgaa aaaccaccgc gacgcagaag gcgaagcgcg
     9601 gttaacgact gaccccgtca gcgggcggcg tgcgcggtgt tccggtttga ctccgtgacc
     9661 gtttacaccg cgcacccacc gcccgatttt tttcaggagt gaaccccatg agtatggttg
     9721 ccagaactga acccagaccc gcagaggacg acatcaccga taccgatgat ggcgataccc
     9781 gcatttcagc gggtgcattc tggccggata ttgtgttgcg cgagctgcgt ctggcggtac
     9841 gactgccggg gcgcgtgacc acttcccgcc tgctacatac cgccaccgga gctgtggcac
     9901 acgttacccg cgagctggaa gcatggcagc aggaacagca ggcggctggc catcagacgc
     9961 tggccgatgt tccggcaccc gtaattaacg gagaaagcgt caatctctgg cactggcgca
    10021 atgctgttta taccgccacg cgcgccctga ttctggagcg ttatcgtgat gcagacacaa
    10081 cggacaaggg cgaccgccgg gcggacgctc tggatataca gacatcggat ttgtggcgtg
    10141 atgtgagctg ggccatctct gacattctgt gccgcccgcg aatctttgcg gagttgtgct
    10201 gatgaaagtg aaggcactgg aaggcgacac cgtggattcg ctctgtttcc ggtactacgg
    10261 cacgacgcag ggcgtcaccg aaaaggtgct ggatgccaac cccggactct gtcagcaggt
    10321 atttctggac gccgggcagg aagtggagat gccggagccg gagaagaaga aacgagaaat
    10381 gattcagttg tggggggcgt agcagtgagc accattcaaa cagggatcac agagcaggtt
    10441 attgcgtggc tctttgacca cctgccaacg gtgtatgcag taggcgcggc ggtcagcatt
    10501 tccgcgctga tgagtcttta tgacggacga acactggttc agaccgtaac gggatcgctg
    10561 gcgtgcggcg ttcttgccat ggccgtggcc gggtcgttgc gcttcttcgg ttttcctgaa
    10621 gatgccgtga cgtttatcgg cgcatcaatc ggttttatgg gggcagagaa agcacgcgac
    10681 aaggttattg cggcctttaa tcgcagggtg aaggagaagg acgaatgagc aacacattta
    10741 aattcagcag ccggagcgaa aagaatttgc agggcgtaaa tcctgatctg gtgaaagtga
    10801 cccgacgggc actggaaatc tcggaagtgg attttggtat caccgaaggg ttgcgcagcc
    10861 gttaccgcca gaagcaactg gtgaccacgg gtaagagcca gaccatgaac agccgccacc
    10921 ttacggggca tgccgtggat gttgtggctt atatcggcag ccaggtgtca tgggaatggc
    10981 cgctgtacga aaaaatcgca gcagcattca gacaggccag ccgggaactg aatattccgg
    11041 tggaatgggg cggcgactgg aaaaccctga aagacggacc gcattttcag ttaccacacg
    11101 gagcctatcc ggcatgaagc tctggcccac gcttggcgtc gctttccttc tgattgccgg
    11161 atggggaaca tccatgcgtc tgtcgtggtc gctgggccgg gagaacgcca gaaacgaagc
    11221 gcaggccagc accctgaaaa gtaccgccga caccctgaat atcattagcg ccggggtaca
    11281 ggatatgcag caggtgctgg cgcaactccg cgtggaaaat caacagagaa atcaggacgg
    11341 agaggccaga cgtgaacagc tacgcaacga tattgcaaaa gatgaatgcg cccacgcttt
    11401 gcctgacgct cgttttactg acaggttgcg caggcacgca gaacgcgcca cggccagcgc
    11461 cgtcagtccg gcttataccg cagacgctga ccataccggt aatgcctccc cccttccctg
    11521 aaactcccac atggggaaat ctcggtatat ggggcgaccg ccttctggat gcacttgaaa
    11581 cctgtaacgc ggataaacgg gccattgaat tactggaaca gcgcaggctg caacgactga
    11641 acaacgagga taacaaccat gctgaaaact gattccctgc gtgaagccat gacccgttca
    11701 tgccgatggt gtcaggctaa cccggaaaaa ttcaccattt tcgtggagag cggcaacatt
    11761 gaaacgaccg gagaaacgcc ctcgtttgtt taccgctatc agatggtgat gtttgtcatg
    11821 gattacgcag gagagctgga cgacctcacg ctgccgctgc tggcgtggtt atccgaaaat
    11881 cagccacagt tgttgctcaa tccggagcgt aatcaggaca tcaaattttc cgccgttatc
    11941 aatgacgatg acagcgccga tctcctgttt acgctccccc tgcgggaacg cgttcgcatc
    12001 acgcgcaaca gtcagggcac accgcaggca gaacacctgc cggagccaaa accccgtctg
    12061 ccctcttccg aaggcgactg gtcgcatgta ttccaggatg tgacgtgggg tgaaagcgat
    12121 ggataaggca ttcacccgcg tggatgaaac ctttgaggcc atccgcgaca gcctgaatca
    12181 gcaggccatc aataacatcg ccagaaagct ggcacaggat ttacgccgcg cccagcaggc
    12241 acgtatccgg tcacagaaag cgccggacgg ggccgcgtgg acacctcgca gacgccgcgt
    12301 aacccggata caggagcgca ttcgctttat ctggaataac gaagcacgca cgctgaaaaa
    12361 ctggcatcac gacacgggga aatacgggcg aaccattacc gggtgggatg aggataaaaa
    12421 caatatccgc acgttttacc gggatgacat cgaccgtttt ctggaaatac gcacccggcg
    12481 catcaaccag gacagcacaa agcgcgtccc catgttcgta aaactgcgca ccgcccgcta
    12541 cctgaaagcc cgtgcagatg cttccggtgt gacggtgggt tacagcggcg tggccgcacg
    12601 tattgcacgc gttcatcagt tcggtgagcg cgatcaggtt gcgccgggca ttttcaccga
    12661 ttacccggta cgtgagctgc tgggtatcag ccaggcagat gaacgcctga tttataacac
    12721 ggtgctgggc cggattgcgg aggctgtacg gtgagcgcag aactcatgcg actgctgagc
    12781 aacatcattc gcaccgggat catctctgaa gttgatgaga agtcctggcg cgtgcgcgtt
    12841 cgcagcggcg aactggaaac aggctggttg cgctggaaca ccacgcgcgc gggagccttc
    12901 aatgtgtggc tgccgccatc accaggcgaa caggtggtaa ttgcctgcat tggcggcaac
    12961 ccggaaaccg ccatgataat tggcagcctg tggagtgatg ccattccggc ccccggcaaa
    13021 agcctgaaag aaatcgtggt cagcgcgccg gatggcgcgg tgttccgcta cgacgcggcc
    13081 gcaggcgcac tgagcgccag cggcatgaaa acagccaccc tgcaggcatc cgtcagtgtg
    13141 acactggata cgcctgtcgt ggaatgcaca gaccttctga gaacagcgac gcttgacgtc
    13201 acaaaagggg gaacgatgag cggcaatatc acgcacagcg gcggcaattt cacctcaaac
    13261 ggcatcacag tgcatacgca taaacacggt ggcgttaaag gtggcagcga ttcgacagga
    13321 ggcccgcagt gacaacccgc tacacaggaa tgaaccagga cggaacggga aacctgaacg
    13381 atatggggca cctgaaacag tcagtcaggg acatcctgac caccccactg gccagccggg
    13441 ttatgcgacg ggaatatggc agccttgtgc ctgatttgat tgacgaaccc atgaataaca
    13501 ccacgcgtct gcaatgcatg agtgctgccg tgattgcgct gacacgatgg gaaccccgca
    13561 ttgccctgga tgccatcgac gttgtctgga aagcgggagg ccgcgccggg gtgacgctgt
    13621 cgggcactgt catgcagacc atgcagaatg ttgagttaac catcacgctg agggagtaaa
    13681 tcatgcccgc cgttgacctt tcccagttac cggaccccgc catcatcgcg gagcctgact
    13741 ttgaagcaat tctggctgac acaaaagcca tgatgattgc gtcctatccc gccgaacagc
    13801 gtgaagccgt ttccgccgcg ctggagctgg aatcggaacc ccttaacgtt atcgctcaaa
    13861 ccatgtcgtt tcgtgaaatg ctgttacgcc agcgggttaa tgagggcgca cgcgcctgta
    13921 tgttaagcca cggttcaggg acaaacctgg acaacctcgc gggcaatatg aacacaaagc
    13981 gcctggttat cactccggca acggatacca ccgacgcggt gatggagagc gacacctcgc
    14041 tgagactgcg ggcgcagcgg gcgtacgacg gcctgagtgt tgctggcccg tcaggtgcat
    14101 acgagtattt tgcccgcagc gccagcggtc tggtgcgtga tgcgcgggct atcagtccgt
    14161 ctccggccaa cgtgacggtt tccatcctgt ccactgaagg cgacggcaca gcaacggagg
    14221 cgttgcttaa taccgttcgc gccgttctga atgcagagga tacccgcccg gtggccgacc
    14281 gccttactgt acagagcgcc agaatcgtga catggcggct gaatgcaaaa ctgtactttt
    14341 accccggccc ggaatccgaa cctattctgg ccgcggcgga atcgtcattc aggaagtggc
    14401 tggctgaaca ggggcttatc ggtcaggacg tggcgttgtc cgccattgct gccgcactgc
    14461 atgtgcacgg tgtgcaacgc gtggagataa tcgaacccac acagaatatg gccatcagcg
    14521 acatacaggc ggcgcgctgt gagtcattca ccatcagcga aggtgggcgc aatgagtaat
    14581 tcgttgttac caccatcagc cagcaatttc atgcgttgcg ccgaagccgt cggaacacgc
    14641 attacagaca ttccggtaga cctcaacacg ctgtggtcgc cggacacatg cccggcgcat
    14701 ctgctgcctt atctcgcctg ggcgttttcc gttgaccgct gggatcgcaa ctggccggaa
    14761 gagacaaagc gacaggtgat tcgtgatgca tggctgatac accgacacaa gggaaccatc
    14821 agcgcactgc gccgggccat tgagccgctg ggatacctca ttcgcgtgtc tgagtggtgg
    14881 gagttcggcg gagaaccggg aacatttacc gttgaagtcg gcacgctgga cagtggcgtg
    14941 acggaggaaa tgtatctgga aatggagcgg ttgatagctg atgcccgccc ggtcagccgc
    15001 cacatgacag ggctgaatat cattcaggaa attccgggag atattttcgc ggcggcagca
    15061 acttatgacg gtgaagttat taccatttat ccggacgatt aagcatgagt accacaacac
    15121 gaaaatttaa aaccgttatc accgatacgg gtgcaaaaaa attagctcag gcagccgcgc
    15181 cagatggtaa gcccgtccgc ctgactcata tggccgtggg cgacggtggc ggcacgttgc
    15241 ccacaccaga cagtaagcag acccgtctgg tgcatgaggt gtggcgacac actgttaatc
    15301 gcgtcatcct ggacgcaaca catcagaacc gcattattgc ggagctggtt attcctcctg
    15361 aaacgggcgg attctggatc cgggaaattg gtgtgtttga tgagcacggc gatttaatcg
    15421 cggtgggcaa tactgccgaa agttacaagc caactgttgc cgaagggtcc ggacgtgcac
    15481 aaacatttcg caccattctg accgtatcca gcacagccac cgtggcgctt accgtggata
    15541 acaccatggt gatggccaca gtggattacg tggatgacaa actgaaagag catgaacagt
    15601 cacgacgtca cccggacgcc tcgctgaccg caaaaggctt tgttcaactc agtagcgcca
    15661 ctaacagcgt gtctgaaacg caggctgcaa cgccgaaagc agtaaaggca gcgtatgacc
    15721 ttgctaacgg taaatatact gcgcaggatg ccagcacgac gcgaaaaggc cttgtccagc
    15781 tcagtagcgc caccaacagc acgtctgaaa cgcaggctgc aacgccgaaa gcagtaaagg
    15841 ccgcgtatga ccttgctaac gcaaaatata ccgctcagga cgccacgacg gcacaaaaag
    15901 ggatagtcca gctcagtagt gccaccaaca gcacgtctga aacactggcc gcgacatcga
    15961 aagcggttaa ggcggtaatg gatgaaacga acaagaaagc gcccttaaac agtcctgcgc
    16021 tgaccggaac gccaacaaca ccaactgcgc gacagggaac gaataatacc caaatcgcaa
    16081 acacggcttt cgttatggct gcgattgccg cccttgtaga ttcgtcacct gatgcactga
    16141 acacgctgaa cgagctggcg gcggcgttgg gcaacgaccc gaattttgcg accaccatga
    16201 ctaacgcgct tgcgggtaag caaccgaaag atgccaccct gacggcgctg gccgggcttg
    16261 ctactgcggc agacaggttt ccgtatttta cggggaatga tgtcgccagc ctggcaactc
    16321 tgacaaaagt cgggcgggat attcttgcga aatcgaccgt tgccgccgtt atcgaatacc
    16381 tcggtttaca ggaaacggta aacagggctg gtaacgccgt gcaaaaaaat ggcgatacct
    16441 tgtccggtgg gcttactttt gaaaatgact caatccttgc ctggattcga aatactgact
    16501 gggcgaagat tggatttaaa aatgatgctg atggtgacac tgattcatac atgtggtttg
    16561 aaacaggcga caacggcaat gaatatttca aatggagaag ccgccagagc accacaacaa
    16621 aagacctgat gacgctgaaa tgggatgcac taaatattct ggtaaatgcc gtcattaatg
    16681 gcagtcttgg agttggtacg acgaatgcgt taggtggtag ctctattgtt cttggtgata
    16741 atgacaccgg attcaaacag aatggtgatg gtattctgga tgtttacgct aatagtcggc
    16801 gagtattccg ctttcagaat ggagttgcta ttgcttttaa aaacattcag gcaggtgata
    16861 gtaaaaagtt ctcgctatcc agctccaaca cctccacaaa gaatgcaacg tttaatttat
    16921 ggggtgcttc aacccgtcca gtagttgcag agttaggcga tgaggcagga tggcatttct
    16981 atagccagcg aaatacagat aactcggtaa tattttctgt taacggtcag atacagccca
    17041 gcaactgggg gaattttgat tcacgctatg taaaagatgt tcgcctgggt acgcgtgttg
    17101 ttcaattgat ggcgcgtggt ggtcgttatg aaaaagccgg acacgcaatt accggattaa
    17161 gaatcattgg tgaagtagat ggcgatgatg aagccatctt cagaccaata caaaaataca
    17221 tcaatggcac atggtataac gtcgcacagg tataaattat gcagcattta aaaaatatta
    17281 agtccggaaa tccaaaaaca aaagaacaat atcagctaac aaagaatttt gatgttatct
    17341 ggttatggtc cgaagacggt aaaaactggt atgaggaagt aaataacttt caggacgaca
    17401 ccataaagat tgtatacgac gaaaataata ttattgttgc cataaccaaa gatgcctcaa
    17461 cgcttaatcc cgaaggcttt agtgtcgttg agattcccga tataacagcc aatcgtcgtg
    17521 ccgatgattc agggaagtgg atgtttaagg acggagctgt ggttaagcgg atttatacag
    17581 cagacgaaca gcaacaacag gccgaatcac aaaaggtcgc gttgctttct gaagctgaaa
    17641 gcgttattca gccactggaa cgcgctgtca ggctgaatat ggcgacggat gaggaacgca
    17701 cacgactgga gtcatgggaa cgctacagcg ttctggtcag ccgtgtggat acagcaaatc
    17761 ccgaatggcc acaaaagcct gagtaaaaat taaggcccaa tatcgagcct tctctcattc
    17821 tggttgttcg ggaaacgtta ctggcaggct ggaggtgtct gtagattcga ctttctgcgc
    17881 atagagcatc cactcggtta atttttgttt attctcgtcg gaaatgatgc ccagccgtag
    17941 ctgtgagtcc catagctggg ttttatccct gacaagttgc aacaggcttt gcttttcatt
    18001 ttccgcttgt tgcctctgct cttcctcggt ataagttcgc tttatcacta cgccatcttt
    18061 gaacaaccat ttccccgaaa tatcagcccg gcgatttgct gtaatatcag gtaattcaac
    18121 gacgcttgcg ccttccggat taattgctga aacatccttt tcaatacaaa taataacgcc
    18181 gttatggtca tagaccattt tcaaagtgtc tggctggaaa ttcttttgtt cctcatacca
    18241 gttttttcca tcatctgaat aaagccattt gatgttaaat tgctttgtta gctggtattg
    18301 ctcttttgtt ttagggttgc cagcagtaat gttttttaag tgcatcatcg ttaaatactc
    18361 cccgcgttat accacgtccc attaatgcaa tactgaattg gccttgcctg agttgtatca
    18421 attaattcat cacggtttcc gttaactgaa cccgtaacga cataacctga cctgtcagac
    18481 cagccgggac cattccatgt ctgaacagat gacagaccgc caaggcgaat acctgtaata
    18541 aaccttgagt tacattctgc ctgcgtgtat gcaccaacat ctcccgcaga gggtttgcgg
    18601 gtcgtggtgt aaaactctga ccagtcggct tcaaaaccat aaccatcacg ggctgaacga
    18661 taaaaaatac cgccattttt ataattaatc cgaaactgag ctgcaggaca acttccttct
    18721 cccatataaa aatgaataat taacgttgat gcaccactaa tagttgcgtt ataggctccg
    18781 ctactccagt tccatccaac tgctttatca ttcgcaacgg tgcttcctgt tttccctaag
    18841 gcaaacgcac caacatgact tgcttttaat gtgatatcgg aggaaccatc aaaagccaca
    18901 ttgcttattt tcctggcagt ttttaatttt gcagctgtag aagcattgcc ggatagttca
    18961 ccagaaaggc caccgctgaa tgtttgtcga ttagtccagg tattcgctgt actgagtaac
    19021 ggtattttct ccccgcttgt gccgagttct cgtaaaccga ggttttagat aatggccgtt
    19081 tctgacctgc atggcatgat ttacgctttt ggacgggaga ttcagcgtgc tgattggcta
    19141 tgtaagggta tcaacaaatg accagaatac agacctgcaa cgaaacgctc ttgtttgtgc
    19201 aggatgtgaa caaatatttg aagataaatt aagcgggaca aagacagacc gaccgggatt
    19261 aaaacgcgct ttaaagcgcc ttcaaaaagg tgacacgctg gttgtctgga aactggatcg
    19321 cctcgggcga agcatgaaac atctgatttc tctcgtcggg gaactacggg agcgagggat
    19381 taattttcgc agtctgaccg acagcataga tacatcttct ccaatggggc gttttttctt
    19441 ccacgtgatg ggtgccctgg ctgaaatgga acgtgaatta attgttgaac gtacactggc
    19501 cggactggcg gcagcgcgtg cacgggggcg cacaggcgga cgtcgaccga agctgacaaa
    19561 agaacagcat gagcaaatag caaggctgat caaaaacggg cacgacagaa aacaactggc
    19621 aataatttac ggcattggca tatcgacgat ttatcgttat caccctgtag gcgatataca
    19681 ggctgaagaa acaaccaggc agactcagga aaatgaaaac cgctaatctg accattagcg
    19741 gttttgcgtt aatcaaaaca gccctttaac ggagctggcc gcgctgttaa gggatgatgt
    19801 gaccttatct ttgaagccgg acagcatatc actgaacgat gaggattgca ggcgctcccg
    19861 caaatcctca tcacagcgtt caagggtcag tgaaaattct atctttttcg ccttaccgta
    19921 gcgatcaaac tcggaacggg tcgtattcgt tccggtcagg acatacatgc cgtaaatctg
    19981 cccgacgcca tcaatcagag gccaggggcg tcctgtatac gcctgcgtgg tcagcagcga
    20041 cagcgacact tcgccacctg taatttcagg ataaagcaca ccagaaagaa cgatgcgatc
    20101 atcacctgca ccgatatact gccagcttgc tgaacggtta acgcgttcat ttttcacatg
    20161 ccgccagctt ttgttttgct gtaactgctg atgcggcagc gtgcgcagct caaaaacaaa
    20221 catgccgtag atcatcatca tggccatgac tcctcaatct ttatcgtaaa aactgccacg
    20281 cccggcacgg gtgcgccgtt ccatttctgc cctgaccatt tcaccgacca gtttcgccag
    20341 ttcgcgggga ttctgcgtaa caacgttatg cagatgaaca tgaatttcac caccaaatcc
    20401 ggaggcaaca ggctcccggt tacgggaagt tacaggaact gatgccactg gagatcgtat
    20461 agcctccgcc accgggcggg agctggccgc aacaacaggg accagcgccg gaggcagcgg
    20521 agccgggacc acgggtgtga tattaattgc gggggcaggc ttactgacct gcgcaatctt
    20581 ccgctcctgc cactccccac gaacagcaag tgctcggggc aggtttttaa agacaatatc
    20641 gccggggcca atgcgttttt tcgtctcctc aaccagctta cctgtgttat cagcaatttt
    20701 gctgagtctg cgcagcgtac cggtattgct gtctgtgagc ggtttattgt ctttggggtt
    20761 atcacctccg gtgccattgc cattttccac aggcttcggc ggattgattt tcgccaggtc
    20821 cccctgaagc aaggcaacct tgtcctgaag aatggccgca cgctgtgcgt cttcgatttt
    20881 cttgcgcgcc ctttccgctt catccggaag cacaccaagc ttttcaagta tccacgccag
    20941 cgtatccagc aacatttttg caggtgtcag aacaagttgt aacgcaccgc caagaacgtt
    21001 accgaatatc tcgccagcac tggtacattt atccagagtt tccttgctgg actccatcgg
    21061 tgacagcagc gatttaaacc agttaaacac ctggctgatc ccgcttccga ttgcgtcaaa
    21121 aacagggcca aaccgttcaa aggtttcacg caacgggttc agcctttcca taatcccgct
    21181 gaacaccccg gcaaaaaatg ccctgatggg atcccagtat ttccagataa gaacggcagc
    21241 tccggcaagc gcagccacga taagaccaac cggactgaac agcgccccga tagcgcctcc
    21301 cagcaaagaa acggaacccg tcagcattcc ccacagcgca ggcaacaccc tgacgacatt
    21361 cattgaccgg gtaagaatgt caaaaccaag acgcagggtg gccagctttc cgtaaagcac
    21421 cccaataacc agcgacaacg agccaatcgt tgcagtcatt gccagcaacg caccgcctgc
    21481 tatcagtagc tggcgtgtca gtaccggatg ggcctgcgcc agcgaggtga ttttttcaag
    21541 cacccgcgtg agccactgcg tgacagaacg cagcggaccg tcaaccagat cactgatgcg
    21601 aatacgaaga ccttcccatg cgctgtcgag atttttcagg tccccatcaa gattatcggc
    21661 cattactttt gcaacgcgat cggcctctcc ccttgccccc tgcaattctc tggtcagttt
    21721 ttgcagctct cctgaaccag ccgccgcaac aagcgtctgc aaaccaacga acgcctcttc
    21781 tccggcgatg tccttgaaga aggagacctg gtccacctgt ccgtattttt gtgtcgcctt
    21841 atagagatca agcagcacat cctccatcgg gcgcattttg cctctggcgt cagcaactga
    21901 cacccccagc tctttcagtg catcagccgc agcttttggc ggtgatgcaa ggcgggacag
    21961 acttgcgcgc atggccgtgc cagcatcgct tccgcgaaga ccattattgg caagcatccc
    22021 ggccatggcc gccgcttctt caagactgat accaagtttt gcggcaaccg gaccggtata
    22081 cttcatggtt tcgcccagcg cgcgtaaatc agtattggtc cgggtaaatg ctgctgtcag
    22141 cgtatcgccc acccggtcca tttgatcggc tgtcaggttg aactgtgtga ggatattgga
    22201 gcctatatcc gccgtctcgc cgagttcgac gccacctgcc agcgccatat taagaacacc
    22261 gggcaatgcg gcctgaatgg cctgcggagt aaaaccagcc attgccagaa agctctgccc
    22321 actggcggca tcactcgcag taaactgtgt ttcagagcca agttttaacg cctgctcacg
    22381 cagcgcctta aactgcgggc tgtttttgtc gattcgcgtc agtgcctgaa cgcgggacat
    22441 ctctttgccg aacccgatcg caggctgcaa aaaacgcccg gcagcatagc cgcccgccgc
    22501 tgccgcacca attgccagcg caccacctgt tttcagtttt cccgcggttt cctgcgcgcg
    22561 cgaataccgc tcacgcgccc gcgttacacg cgcaagcgcc tgccgttcgc gttcaagctg
    22621 gttgttgtac tgttcggtgc gtctgatggc ctgctggatg gtgttatcgc tgcctgtcag
    22681 ggaaatgccg tggcgtttca gctctccgcc aagctcccgc attttctgaa tttcccgtgt
    22741 gcgcgattca ttcaggcgtt caagccgggt gcttaactgc tgcatcagct tttgttgttt
    22801 ttcgctgagc actgtacccg tgcgttgtaa ctgattaagg gcgttaagct ggcgtcgtgc
    22861 tttcacgata cccgcatccg ctttactgac agcgtcgcgg gcgcgctcaa atgatcgcgc
    22921 ctgacgctcg agatttttga tcgccccctg cgttcgctgg atggagtcac caaactgccc
    22981 catcaggcgg cgggcgtttt cggcaggccg ggtcagcctg tcaacggcgc tgaaagcgac
    23041 ccggatatca agagtcttca ttgtctgcat tcccgctgcg aagtgccgcc cgctcacgcc
    23101 agctaaccac ttcgccgggc gtcatcatga agatttcggc gggcgaccag ttaaaaatgg
    23161 cggcaatatc cgccacaaag tcttctatgt gctcaaagca cacaaccgtg atcaggcttc
    23221 cgtcgcctgt tcgttcttcc cgccagagtc cgcaccgctc aaaaaattta cggcaaccac
    23281 acataactga ataaaatcac gggatgccat ttttttgatc gtcacttcat ccagtcgcgg
    23341 tgatgtcacg cgtgacagca gcgtaaacat ggattccgct ttcagattca gcacatcaga
    23401 cagcgacaaa tctcgcagag atccagcctg ctcaatagcc ccggtgatct ccacatacgt
    23461 gattttttcg ccgcctcgct caattggttg ggtaagtttt acgccacgct cactggtttc
    23521 tttcacagtg tcagcaacga ccgtgttttc ggtatcgatg tttttcgtct ctttcatcag
    23581 gaaactcctt tcagtcagag gcgacgcact gcgccgcctg catattactt atcagccaag
    23641 cccgagcgcg gaacggatgc gatcgggcac aatgtccttg ccgtccttcc ggtaaatgaa
    23701 gttcagcagg tcaatctccc acaacgggcg atcgttaaca ctcagcttgt agtaggtgtt
    23761 tttaatggcg taagtgtgtg atgtggcttc gccctgtttg gcttccccca tatcaatttc
    23821 cgtcacacgt ccgcgcattt cgacttcata caggtcgctt tctgcatcgg tgtagtattc
    23881 acccgcaaaa cgcagcagcg tgccgtcaat cgtgccgcca tacttaagga acagctcacg
    23941 aactgcgccc cccatgacaa agctcgcatc aagcgcggag tcgtccagac cgagatcaat
    24001 acttaccgca cccatcatgc caccaccccg gtagctgtcg gttttgcgcg tcagcttagg
    24061 cagagtgacg gacgtcacct tacccacttc gttttcacca tccacaaaca gcgtaaaaaa
    24121 gcgaagatgt tttggtacag ccatcaggca cctcccagca ccgcaaatgc gggaccaaag
    24181 aattcatcag taaacgactg gtaaagctcc atgtcttcca gcgggggaac aggcgtatat
    24241 ttgtagcgaa tacgcacgcg cccctgacgt aaattcgtgg tgctgttatc cacgatgtca
    24301 taccagcact ccgcgccaat cagtttcccg gcagtaacca gtgaatccag ttttgccctg
    24361 atggcactga taacatcttt cacgttcgca ggcgtcagtg gactgtcgat ggtttcaaac
    24421 tgcgcttccg caattgaatc agccagcacc tgtgcggttc gggtatacac ctcaaagatg
    24481 taggcgttcg tttccggtgt gcggttgccc cagaagcgga acccgttgcg acgaataatg
    24541 gtcgtgattt ctttgttgtt gaggctgttg gcatcactgt cttcggcctg caacgaccag
    24601 aacacatgcc tggacatccc cagcacattt ttaaccggaa cgttggacag cgatttgtgc
    24661 cagccctgct catggtcaat gtacgcacga aggccgcacg cataggcagg cgcggggaac
    24721 gtttcgtttt tgccactttt cgggttgtag gcgatgaagt ccggccataa gagcatcacc
    24781 tcacgctcgt tgaatttctg gcggtaggta atcgcctcag ccatcgtgtt gcagccgtga
    24841 catgaggcat acacaaacgc gcgcagttta cccgcaatca cgcacaggga ttttgttacc
    24901 gcctccgtgt ccagctccgg cgcggccaga atacgcggac ggtatccgat gctttcatcc
    24961 tgctctgcaa caagcagcgc atacatcccc gtatagctgc cgtcatcctc agaaccaccg
    25021 ataaccagtt gatcctgcgt ctttccgtct tcttctttgt gttcagccac gcgaacgacg
    25081 atcacctttg tgctcacctg gtctgcgatg gccttaagcg cacgataaag cgtccccgtt
    25141 gttccgcatt ttcccagcac gtcattgacg cgggtcagca gtgtgggctt gttcagcggg
    25201 aacagctccg catccgcatc atccgccgtt gccacgatac cgataacact ggaatcaaca
    25261 tcattaatcg ctgttaccag gtcggtactt tccgtaacac gggcaccatg aaaacgagtt
    25321 tcactcatag cttcagcccc ttgtatccgt taaatgattc agcaacaatc atcacccacc
    25381 acgcgcgtaa tctcacccct gcgctattct cccgccacgg cgacaacaaa aagcagtaac
    25441 ccactccgca cgcacatgcg accatgccgc acagggaggg aacagatgac cgacaccacc
    25501 atgcaattgc tcagtcaggg cacagacccc gtgaaaatgc cggattttga tattctcgcg
    25561 gagggtaaaa cgctgtccgg cgtggcagag cgcctgatga gcctgtcgct gaccgacaac
    25621 cggggatttg aggcggacca gctcaccatc acgctggatg atgccgatgg ccagttgcag
    25681 ctaccgccac ggggcgcgcg tctgacggtt ctcattggct ggaaagggga accgctgaca
    25741 gaaaaaggca cttacattgt tgatgaaatc gctcacgaag gaccgccgga caggctgact
    25801 gtttcagcca gaagcgcaga ttttcgggat gaatttaacg ttaaacgtga ggtgtcctgg
    25861 catgatgtga ccgttgagcg tgtggtatcc gccatcgctc atcggtacgg tctgaaacca
    25921 caaatcagcg aaatgctgat ggatatcgaa atcgaccacg ccgaccagac cgaagaaagc
    25981 gacatgtcct ttcttacgcg catggcggaa atgctgggcg caatcaccac ggtaaaaagc
    26041 ggtaatctgt tattcattat gccaggcggt ggcgtgaacg cacagggcca gccgttgcca
    26101 tcgttcgcca tcacgcgcag cagtggcgat cgccatcagt tccgcattgc tgaccgcgaa
    26161 gcgtatacgg gggtacgcgc ttactggctt gatcttaatt acgggaaaaa gaaaaaagtc
    26221 agcgtgaaac gccgcaaacc accaaaaccc aaaaaggaga aaagcagcag ccgtgaaggt
    26281 gattatatgg aaggtgcgga aggcaatgtg tttgtgttac gcaagactta tcagaacgag
    26341 caggcagcaa gacgcgcagc ggcggcaaag tggcagcagc tacaacgcgg agccgcatca
    26401 ttctccatca cactggcacg tggacgcgca gaactctacc ccgaaatgca tggcacggta
    26461 acaggattta aaagcgagat tgataatcag gactggatta ttgcaaaagc cgagcacacc
    26521 attgataaca gtggctttac cacacagctt gagcttgagg caaaaatccc ggaatggata
    26581 gcggaaacag agtgaggaac ttagaatagc gacagcacca cgttaaggga ggtcgctatg
    26641 ttccgttgtc cgctttgtgg cgcatctgcc cgtatccgca ccagtcgtcc ggaaaatgat
    26701 tcaaacaccg tgcggcaaaa gtattaccag tgtaacaatc tggaatgcgg cgtatgcttc
    26761 tcaacactgg aagctttcca taaattcaca tcaaaacacg cctccggcgt tcactcttca
    26821 gaaggtatcc cgtggcatga gctgccagct tcacacaggg gaaacaatca gatgagtttg
    26881 cctttatctc agaattaaca agcagaattg ccggagtaac aaaaaagcga tagattacgc
    26941 gcgggtgcct ttcggctgat ggtcggaggg aatacccgaa ggccagatgt ggaaaggccc
    27001 cggaaaacat ttctgtttaa ccgaggccct aaccgtctaa ccttagcaag tgataggtta
    27061 gcgcctctcc aacaaaggag caagcgctat gtcgcaaaaa tcgcttacgg ccatcacgtt
    27121 ctgcgtgacg gtaatcctca tcatctggat gctgcacggt tcgctgtgtg aaatacggat
    27181 gagcttctgg ggagcggagt ttgcggcgtt cttacagtgt aagcagtaag gaaaccgcga
    27241 cggggaggca actccccgtc aatcggttgc tagggtaagg ccgataaggc accctatctc
    27301 ataggcatga ataacaaccc cgcagcgtaa aaactgcggg gtttcttttt ggaactctca
    27361 ctagctagtg aggcgttaac tgcacataat gctcgcacaa caaatcagtt aaaagagatt
    27421 actcagcatt ctcctgcttc cattgccgga tcatttcatc ggtaacatca ctttcataac
    27481 ataccacgtc ataccctcca gtacggctat atgcactgcg tccaccacat ctactaccat
    27541 tccttgcatg attatacgga cacgcacaat tgcctggata agattcaatg gattctttaa
    27601 ttatttcttt cttgatctga gcatctgact ttcctgttgc tgcataccca ctaaaagata
    27661 ccaaacaaag gcagattgcc attaataaac cacatcgcat accgatacac tctattacta
    27721 accaatcata gcccacatca taaaatcgag atgaggagta caacaccttt aacaagaagg
    27781 tgccatcacg ataatatgcc gtgcaattca tcaaaaaaaa gatctatatc aaagagatag
    27841 agttattatc agtgctgcag acatcagcat tcgaggcagc agaaagacag taaatctaac
    27901 gaatttgtct ttttctcagg atgttgtcag attaaaaaag atagagtagc gcagaaacct
    27961 tttggtggat ggtaggagga aaaatgaagt tgagtgggaa gatcccagaa aaaactttgg
    28021 tttaaccaag gccctagttc aatatgcgaa atgcagatga gttgcaagag agttttgctt
    28081 cacccttaga gtttaaacag acgtgtacta ttattatttc tgcatatcgc cacaccatcg
    28141 ccactttacc gccatttaac aaaatacaaa
//
LOCUS       DAZFRG010000038        26727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFRG010000038 DAZFRG010000000
VERSION     DAZFRG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.038"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            235..348
                     /gene="yhiY"
                     /locus_tag="ACHKUC_001286"
     CDS             235..348
                     /gene="yhiY"
                     /locus_tag="ACHKUC_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO6617571.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            411..1478
                     /gene="yhiI"
                     /locus_tag="ACHKUC_001287"
     CDS             411..1478
                     /gene="yhiI"
                     /locus_tag="ACHKUC_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6617572.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWLLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1475..4210
                     /gene="rbbA"
                     /locus_tag="ACHKUC_001288"
     CDS             1475..4210
                     /gene="rbbA"
                     /locus_tag="ACHKUC_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO6617573.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4210..5334
                     /gene="yhhJ"
                     /locus_tag="ACHKUC_001289"
     CDS             4210..5334
                     /gene="yhhJ"
                     /locus_tag="ACHKUC_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6617574.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5343..5744)
                     /gene="nikR"
                     /locus_tag="ACHKUC_001290"
     CDS             complement(5343..5744)
                     /gene="nikR"
                     /locus_tag="ACHKUC_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO6617575.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(5750..6556)
                     /gene="nikE"
                     /locus_tag="ACHKUC_001291"
     CDS             complement(5750..6556)
                     /gene="nikE"
                     /locus_tag="ACHKUC_001291"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO6617576.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(6553..7317)
                     /gene="nikD"
                     /locus_tag="ACHKUC_001292"
     CDS             complement(6553..7317)
                     /gene="nikD"
                     /locus_tag="ACHKUC_001292"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO6617577.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(7317..8150)
                     /gene="nikC"
                     /locus_tag="ACHKUC_001293"
     CDS             complement(7317..8150)
                     /gene="nikC"
                     /locus_tag="ACHKUC_001293"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO6617578.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPVQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(8147..9091)
                     /gene="nikB"
                     /locus_tag="ACHKUC_001294"
     CDS             complement(8147..9091)
                     /gene="nikB"
                     /locus_tag="ACHKUC_001294"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO6617579.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9091..10665)
                     /gene="nikA"
                     /locus_tag="ACHKUC_001295"
     CDS             complement(9091..10665)
                     /gene="nikA"
                     /locus_tag="ACHKUC_001295"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6617580.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(10776..11363)
                     /gene="acpT"
                     /locus_tag="ACHKUC_001296"
     CDS             complement(10776..11363)
                     /gene="acpT"
                     /locus_tag="ACHKUC_001296"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO6617581.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYQSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(11418..12467)
                     /gene="yhhT"
                     /locus_tag="ACHKUC_001297"
     CDS             complement(11418..12467)
                     /gene="yhhT"
                     /locus_tag="ACHKUC_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6617582.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            12566..13816
                     /gene="yhhS"
                     /locus_tag="ACHKUC_001298"
     CDS             12566..13816
                     /gene="yhhS"
                     /locus_tag="ACHKUC_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6617583.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(13820..14377)
                     /gene="dcrB"
                     /locus_tag="ACHKUC_001299"
     CDS             complement(13820..14377)
                     /gene="dcrB"
                     /locus_tag="ACHKUC_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO6617584.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(14450..15115)
                     /gene="yhhQ"
                     /locus_tag="ACHKUC_001300"
     CDS             complement(14450..15115)
                     /gene="yhhQ"
                     /locus_tag="ACHKUC_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO6617585.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15336..15581
                     /gene="tusA"
                     /locus_tag="ACHKUC_001301"
     CDS             15336..15581
                     /gene="tusA"
                     /locus_tag="ACHKUC_001301"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO6617586.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(15850..18048)
                     /gene="zntA"
                     /locus_tag="ACHKUC_001302"
     CDS             complement(15850..18048)
                     /gene="zntA"
                     /locus_tag="ACHKUC_001302"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO6617587.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(18122..18748)
                     /gene="yhhN"
                     /locus_tag="ACHKUC_001303"
     CDS             complement(18122..18748)
                     /gene="yhhN"
                     /locus_tag="ACHKUC_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO6617588.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            18889..19248
                     /gene="yhhM"
                     /locus_tag="ACHKUC_001304"
     CDS             18889..19248
                     /gene="yhhM"
                     /locus_tag="ACHKUC_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO6617589.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19251..19520)
                     /gene="yhhL"
                     /locus_tag="ACHKUC_001305"
     CDS             complement(19251..19520)
                     /gene="yhhL"
                     /locus_tag="ACHKUC_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO6617590.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19510..20106)
                     /gene="rsmD"
                     /locus_tag="ACHKUC_001306"
     CDS             complement(19510..20106)
                     /gene="rsmD"
                     /locus_tag="ACHKUC_001306"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO6617591.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            20256..21752
                     /gene="ftsY"
                     /locus_tag="ACHKUC_001307"
     CDS             20256..21752
                     /gene="ftsY"
                     /locus_tag="ACHKUC_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO6617592.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     ASEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            21755..22423
                     /gene="ftsE"
                     /locus_tag="ACHKUC_001308"
     CDS             21755..22423
                     /gene="ftsE"
                     /locus_tag="ACHKUC_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO6617593.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLIGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            22416..23474
                     /gene="ftsX"
                     /locus_tag="ACHKUC_001309"
     CDS             22416..23474
                     /gene="ftsX"
                     /locus_tag="ACHKUC_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO6617594.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            23719..24573
                     /gene="rpoH"
                     /locus_tag="ACHKUC_001310"
     CDS             23719..24573
                     /gene="rpoH"
                     /locus_tag="ACHKUC_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO6617595.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            24845..25948
                     /gene="livJ"
                     /locus_tag="ACHKUC_001311"
     CDS             24845..25948
                     /gene="livJ"
                     /locus_tag="ACHKUC_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO6617596.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(26136..26519)
                     /gene="panM"
                     /locus_tag="ACHKUC_001312"
     CDS             complement(26136..26519)
                     /gene="panM"
                     /locus_tag="ACHKUC_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO6617597.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6256 a         7027 c         7468 g         5976 t
ORIGIN      
        1 atcaggcgat aataacgcta tgtgatagtc atcaggtttc cctgttacgg aaacctgtgc
       61 aacagaatct gtgaacctgt acagaatatt tttggaaaaa acaccagcga acggaagaac
      121 atcgcgatgt atcacaagtt gatggcaatc aaatagacaa catgaatata actgttagta
      181 tttaattgat ttcacttatg agtatgcgtg tttcatttac ggccaggaat gctgatgatg
      241 acaacattac tacccgtttt cactaagcct tccccattgg cgctcaatgc tctgtgcgct
      301 ggtcgtattt gccatttcct tcttatcccg gatggaagaa tccgctgaac atcgttcggt
      361 ggcagttgta acacgcgtcg attgcttaca gggattatgg cggatcgatt atggataaga
      421 gtaagcgcca tctggcgtgg tggggtgtcg gggcactggc ggtggctgcc gtcgtggcgt
      481 ggttgctgtt gcgcccggcg ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg
      541 aagcgacgga agtggatatc gccagcaaaa ttgccgggcg tatcgacacc attctggtga
      601 aagaaggcca gtttgttcga gaaggtgaag tgctggcgaa gatggatact cgcgtgttgc
      661 aggaacagcg actggaagcc atcgcgcaaa tcaaagaagc acaaagtgcc gttgccgccg
      721 cgcaggcttt gctggagcaa cgccaaagcg aaactcgtgc cgcacagtcg ctggttaatc
      781 aacgccaggc agaactggac tccgtagcaa aacgtcatac gcgttcccgc tcactggcac
      841 aacggggggc aatttctgcg caacagttgg atgacgaccg cgccgccgct gagagcgccc
      901 gagctgcgct ggaatcggca aaagctcagg tttctgcttc taaagccgct atagaggcgg
      961 cacgcaccaa tatcattcag gcgcaaactc gcgtcgaagc ggcacaagcc actgaacggc
     1021 gcattgccgc agatatcgat gacagcgaac tgaaagcccc gcgtgacgga cgcgtgcagt
     1081 atcgggttgc cgagccaggc gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg
     1141 atctcagcga cgtctatatg accttctttc tgccaaccga acaggcgggc acgctgaaac
     1201 tgggcggtga agcacgtttg attcttgatg ctgcaccaga tctgcgtatt cccgcgacca
     1261 tcagttttgt cgccagcgtc gcccagttca cgccaaaaac tgtcgaaacc agcgatgagc
     1321 ggctgaaatt gatgttccgt gtcaaagcgc gtatcccacc ggaattactc cagcagcatc
     1381 tggaatatgt caaaaccgga ttgccgggcg tagcgtgggt gcgggtgaat gaagaacttc
     1441 cgtggcctga cgacctggtg gtgaggttgc cgcaatgacg catctggaac tggttcccgt
     1501 cccgcctgtc gcgcaactgg cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa
     1561 caatatcaca ctcgatattc cggcccgttg catggtcggg ctgattggac cggatggtgt
     1621 cggtaaatcg agcttgttgt cgttgatttc cggtgcccgc gtcattgagc agggcggcgt
     1681 gatggtgctg ggcggcgata tgcgcgaccc gaagcatcgc cgcgacgtct gcccgcgcat
     1741 tgcctggatg ccgcaggggc tgggcaaaaa tctctaccac accttgtcgg tgtatgaaaa
     1801 cgtcgatttt ttcgcccgcc tgttcggtca cgacaaagcg gagcgggaag tgcgtattaa
     1861 tgagctgctg accagcaccg gattagcacc gtttcgcgat cgtccggcag ggaaactctc
     1921 cggcgggatg aagcaaaaac ttgggctgtg ctgtgcgtta atccacgacc cggaattgtt
     1981 gattcttgat gaaccaacaa cgggggttga cccgctttcc cgcgcccagt tctgggatct
     2041 gatcgacagc attcgccagc ggcagagcaa tatgagcgtg ctggttgcca ccgcctatat
     2101 ggaagaggcg gaacgcttcg actggctggt agcgatgaat gccggagaag tgctggcaac
     2161 cggtagcgcc gaagaactgc ggcagcaaac gcaaagcgcc acgctggaag aagcatttat
     2221 aaatctgtta ccgcaagcac aacggcaggc gcatcaggcg gtagtgatcc caccgtatca
     2281 accggaaaac gcagagattg ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt
     2341 tgtcgccgtt gatcacgtca attttcgcat tccacgcggg gagatttttg gtttcctcgg
     2401 ttcgaacggc tgcggtaaat ccaccaccat gaaaatgctc accggactgc tgcccgccag
     2461 cgaaggtgag gcgtggctgt tcgggcaacc ggttgatcca aaagatatcg atactcgtcg
     2521 ccgggtgggc tatatgtcgc aggcgttttc gctctataac gaactcaccg tgcggcaaaa
     2581 cctggagtta catgctcgtc tgtttcatat cccggaagcg gaaattcccg cgcgagtggc
     2641 tgaaatgagc gagcgtttta agctcaacga cgtcgaagat gtattgccgg agtcattgcc
     2701 gctcggcatt cgccagcggc tatcgctggc ggtggcggtg attcatcgcc cggagatgtt
     2761 aatccttgat gaaccgactt ctggcgtcga tccggtggcg agggatatgt tctggcagtt
     2821 gatggtggat ctctcgcgcc aggacaaagt gactatcttc atctccaccc actttatgaa
     2881 cgaagcggaa cgttgtgacc gtatctcact gatgcacgcc ggaaaagtgc tcgccagcgg
     2941 tacaccgccg gaactggttg agaaacgcgg agccgccagt ctggaagagg catttatcgc
     3001 ttatttgcag gaagcggcag ggcagagcaa cgaagctgaa gcgccgccgg tggtacacga
     3061 caccacccac gcgccacgtc agggattcag cctgcgccgt ctgtttagct acagccgccg
     3121 cgaagcgctg gaactgcgac gcgatccggt acgttcgacg ctggcgctga tgggaacggt
     3181 gatcctgatg ctgataatgg gttacggcat cagtatggat gtggaaaacc tgcgctttgc
     3241 ggtgcttgac cgcgatcaaa ccgtcagtag ccaggcgtgg acgctcaatc tctccggttc
     3301 ccgttacttt atcgaacagc caccgctcac cagttatgac gagcttgatc gtcggatgcg
     3361 tgcgggcgat attacggtgg caattgagat ccctcccaat ttcgggcgcg atatcgcgcg
     3421 tggtacgccc gtggaactcg gtgtctggat cgacggtgcg atgccgagcc gcgccgaaac
     3481 ggtaaaaggc tacgtgcagg ccatgcacca gagctggcta caggatgtgg cgagccgaca
     3541 gtccacaccc gccagccaaa gcgggctgat gaatattgag acgcgctatc gctataaccc
     3601 ggacgtaaaa agcctgccgg cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat
     3661 cccgtcaatg ctaagcgccc ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa
     3721 cctttacgtg actcccacca cccgtagcga atttttactt ggcaaacagc tgccatacat
     3781 tgcgctgggg atgctgaact ttttccttct ctgcgccctg tcggtgtttg tgtttggcgt
     3841 accgcataaa ggcagtttcc tgactctcac cctggcggcg ctgctgtata tcatcatcgc
     3901 caccggaatg gggctgctga tctccacctt tatgaaaagc cagattgccg ccattttcgg
     3961 tacggcgatt atcacgttga tcccggcgac acagttttcc gggatgatcg atccggtagc
     4021 ttcgctggaa ggtccaggac gttggatcgg cgaggtttac ccgaccagtc attttctgac
     4081 tatcgcccgc gggacgttct cgaaagcgct ggatctgact gatttgtggc aactttttat
     4141 cccgttgctg atagccatac cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca
     4201 ggagggatga tgcgccattt acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt
     4261 ctgctcggtg ataaagcgat gctgacgctg attgtcttct cgtttacggt gtcggtgtat
     4321 tcgtcagcga ccgttacgcc aggatcgttg aacctcgcgc cgatcgccat tgccgatatg
     4381 gatcaatcgc agttatcgaa ccggatcgtt aacagcttct atcgcccgtg gtttttgcca
     4441 ccggagatga tcaccgccga tgagatggat gccgggctgg acgccggacg ttataccttc
     4501 gcgataaata ttccgcctaa ttttcagcgt gatgtcctcg ccggacgcca gccggatatt
     4561 caggtgaacg ttgatgccac gcgcatgagc caggcattta ccggcaatgg gtatatccag
     4621 aatattatca acggtgaagt gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg
     4681 ttggtatcgc tggaaacacg gatgcgcttt aacccgaacc ttgatcccgc gtggttcggt
     4741 ggggtgatgg cgatcatcaa caacattacc atgctggcga ttgtattgac cggatcggcg
     4801 ctgatccgcg agcgtgaaca cggcacggtg gaacacttac tggtgatgcc gataacgccg
     4861 tttgagatca tgatggcaaa ggtctggtcg atggggctgg tggtgctggt ggtgtcggga
     4921 ttatcgctgg tgctgatggt gaaaggcgtg ctgggcgtac cgatagaagg ctcgatcccg
     4981 ctgtttatgc tgggcgtggc gctcagtctg tttgccacca cgtcaatcgg tatttttatg
     5041 gggacgatag cgcgttcaat gccgcaactg gggctgctgg tgattctggt gctgctgccg
     5101 ctgcaaatgc tttccggtgg ttccacgccg cgcgaaagta tgccgcagat ggtgcaggac
     5161 attatgctga ccatgccgac gacacacttt gttagcctcg cgcaggccat cctctaccgt
     5221 ggtgccggat tcgaaatcgt ctggccgcag ttcctgacac tgatggcaat tggcggcgcg
     5281 tttttcacca tcgcactgct gcgattcagg aagacgattg ggacaatggc gtgattacgg
     5341 ggttaatctt ccttcggcag gcactgcaaa tgcccgtgcc gcacgccgcg ctgggcgatg
     5401 acgtcatcgg caaaatgctg cacgtcgccc atgtcacctt tcaacacggc gatttccaga
     5461 cagtcgtcgt gattaatgtg cacatgcaga gtagcaaccg agagatcgtg gtgatgatgc
     5521 tgggtggaga caatacggct ggctaagtcg cgtttttcgt gttcatacac atacgacagc
     5581 accgcgaaac cttgtgtgcc gtgttgttga gtggcttcct gcgccagggc gctacgcaga
     5641 atatcgcgga tcgcctcgga acggttgtta taaccacgac gctggctcag gctgtccagc
     5701 gtctccagta aatcgtcatc aagcgtgatg gtgactcgtt gcatttgcgt taaacctttt
     5761 ctgtggtgcg acggcgcacg gggaatgcgg gtaataccgc gttttgtagc acacgtccgg
     5821 cgtcagagga aaaggttaat ttatctccca ccacctgggt ttcgacgatt tgtccgttgt
     5881 ccatcaccat cacccgctga cagaagcgtt ccaccaggcg taagtcgtgg gtgatgaaca
     5941 ggcaggcggt gccaaactgt tgttgcagct ttttcagcag gcgaatgacg cccgcctgta
     6001 acacgagatc aaggttcgaa accgcttcat ccagaatcag cagtttcggt tcgaccgcca
     6061 gcgcgcgagc caggcagacg cgctggagct ggccgccgct taactgcggt gggcgtttgt
     6121 cgagcacgct gtcgtcgaga tcgaccgcct tcagcatttc actggcgcgc gccagttgtt
     6181 cggctttttt cagcgatagc aggtggcgca tcggttcacg caggatctcg cgcacggttt
     6241 tgcgcggatt cacggcgctg atggagtcct gaaacaccat ttgaatatca cggcggaacg
     6301 ctttacgttg cgcgcgattg agcttcgcca acggttcgcc gcgccagcta atatttccct
     6361 gtgagggcga ctctaaccct accagcagcc gcgcgagggt actttttccg cagccgctgc
     6421 gccccagcag ggcgacggtt tcaccgcttt tcagggccag ggaaacgtta ttcagcaccg
     6481 cctggtgttg atgtttgccg ctaaatccac cgtgcgcgta gtgatgggaa aggtcggaga
     6541 cgttaagtaa agtcatgatg ccagctccat accgtagagg gcgagatgag cggaaaccag
     6601 gctgcgcgtt accgcatgtt tgggggcgtt aaacagcgtt tctacatcgc cctgttcaac
     6661 aatcttaccg tgagacatta ccgccacatc gtctgccaga cgcgctacca cgcccatatc
     6721 atgggtaacc agcagcattc ccggtgcttg tttttgcata atgctttcca gcagatcgag
     6781 gatgcgcgcc tgtgctacca cgtcgagatc ggtggtcggt tcatcggcga tgataaacgg
     6841 tgattcgcac agcaccgcca tcgcaatcat catgcgctgc aacatgccgc cgctcatctc
     6901 gaacgggtag agcttcagca cgcgcgcggc gttttccagc cccaccgctt ctatggcagc
     6961 ggtaagcgta gcgtcatcgg cgggtttccc cagcgccagg caggtttcac gcgcgtgggt
     7021 gtgcatggtg tgcagcggat taaaggcact gcgcgggttc tgcatgatgg tggcaatttt
     7081 gattccgcgc agggcgcagg gagaaaccgg ttttccatcg gccaaaattt cccccgccgt
     7141 ctggcgaacg ccagcaggca gaatgcccag cgtcgcggcg caggtcagcg acttcccgct
     7201 gccactaccg ccgactaacg ccagcacacg cccacgttgc agggttaacg atacgccgtg
     7261 caccagcggc tgcgcggcct gtagcgcgat attacggagt tcaatctgtt gtggcattag
     7321 tgtgcgtgct ccgtcaccag atgagggtcc agatgatcgc gcagtgcgtc acccaccagg
     7381 ttaaaggcca tcacgctgat aaacagcgcc agccccggcc agaacatttg caccggctgg
     7441 gtccagatat actgacgcgc gtcgttaatc atcacgcccc attcggcggt cggcgcggtc
     7501 acgccgagac cgaggaagga catccccgcg acgtgtagca tcatatgacc gatatccagc
     7561 gtcgccagca ccagcagcga ggggatcacc gcacctgcca gatgatcaat aaacacccgc
     7621 acatggcccg cgccagaaag ccgtgatgcc agcacaaact cgcgctggcg cagtgaaatc
     7681 accaggctac gcaccatgcg tgcataccac gcccagtgcg acagggcgat ggctataatc
     7741 acattggtca acccggtgcc gagcacgccg accataaaga acgacagaat cgaggttggg
     7801 aaggtcataa acatatcagc gacgcgcatg gtggcctgat caacgcgccc gccaatcagc
     7861 ccggcgctgc cgccaataat cagccctaat gtcagcacca acagcaggca ggccattacc
     7921 gagccgagcg acacgcgggt cgctgccatc agccgcgaga aaatatcgcg ccctaaatga
     7981 tcggtgccca gccagtgctg cgcatccggc gagagcaggc gcgacggcaa atcaatagcc
     8041 tgagggtcat acggcagcca ccactggctg gtgagcgcaa tcagcgccag cagggcgata
     8101 atgatcagcg ccaggcgaac cgaccagcgg gaggagagga aaaagttcac gcgtgcgctc
     8161 cttcatgacg acgaatacgc gggtccagcg cggcgttgag caaatcgaca atcaaattac
     8221 agaccacaaa aaccaccacc atcatcagcg taaagcattg gatcaccgga tagtcacggt
     8281 taaaaatcgc cgacaccgca tagcgcccga cgcccggcca ggcaaagatg ttttcgataa
     8341 tcatcgtccc gccaatcagt tcgccaatgt gcatccccac ggcagtgatc atcggcagcg
     8401 aggcattgcg cagaatatgg cggcgttcgg tctgtttgtc gctcagacca cgcagtcgtg
     8461 cccaggtgac gtgacgctga ccggcgacgt ccagcatact ggcgcgcagc aaacgcgcgt
     8521 taatcgccag cgacataaag gcgatggata ccgcaggcaa aatgatgtgc tgccagccgc
     8581 cgtaacccat cgcgggtagc cattgcagat acaccgaaaa cgccatcacc agtaaaaacg
     8641 ccagccagaa gttaggcatc gacacgccga gaaacgcgat gaaacggacg gcgaaatccg
     8701 gcagacgatc gcgatggcgc gccgcccaga taccgagcgg tacggaggtg agcagaatta
     8761 ataccagcgc cgcacctgca agttccagcg tggcgggcag gaaattcagc atatcgtcca
     8821 gcaccgggcg ttggctggcg aatgagatac caaagtcaag atgcagcgcc ttccacaacc
     8881 aggtgccata ctggacgtac agcggctgat ccagccccag catagttcga gtagaggcca
     8941 gcatctccgg cgtcggcggc aggttagaca gacggagata atcgagcgcc gggtcaccgg
     9001 tgccgaggcg cagcatcaaa aaaatgatca ccgaggcggc gagcaccatc gggatcagca
     9061 gcagaaagcg gcgtaataca taacgcaaca ttaaggtttc accggtttaa tctgttcgaa
     9121 cgggatttcg gtggcgatcg gcgcgtaggg gatgttaccc agttccggtt ttgaaaccac
     9181 catcattgag atgtaactga taggcagata aaccgcctcg tcatgcagac gggtcagaat
     9241 gtcgcgatac agcgcctgac gttgcgtttc gtcatgggtc gccagcactt cgccgatctc
     9301 tttatcaatc agcggcttgt cggctaaacc ttgctgtgcc tggaagtcag cgtgcgacgg
     9361 tacgcgcatt gaactcagga aggcgtgtgg atcgtatggc gcgccccagg tgcggtggaa
     9421 aatcatgcca aaacgaccgt cgcgctgacg agcatagata ctgctctctt cttcgccaat
     9481 cagcgagaca tctgcgccaa tctggcgcat atcagcctga atgatttccg ccatcgattt
     9541 gcttaacgca tcggtgccga tgaacgacag ttcaatgcgc agcggctgac catttttctc
     9601 gcggatgtct ttgcctgcag gcagcgtcca accggctttt tccagcaacg ctttcgcttt
     9661 ctgcggatcg tactggcgcg gtttcaggcc gaggttggcg tagggcacag aaggggcaaa
     9721 cagggtgtcg gcgacctgct gggtaccata caacgcgtta tcaatcagcg attttttgtt
     9781 caccgcgtaa ttaagagctt cacgtactgc cagctcgttg gtgggggctt tggcggtatt
     9841 gagcgccagc atcacggttt cgatcggctg tgacagttga gtgtggtaag ccgggttctg
     9901 gctaaagcgg gcgaaggtat cgagcggtaa taacccttcg tttccgtaca gcaggtcgat
     9961 atcgccagtt tcaaacgcca ccgcgcgggt agtcgggtcc gggatcacgt taaaggtgat
    10021 ctttttaatc gccggctttt cgccccagta gttttcgtta cggacgaaga catcgtactg
    10081 attcagtttc gattcctgta aaacccacgg cccggtgcca atcggcgctt taattccgtt
    10141 catggtttcg tggtttttaa actgcgaggg tgcgataaaa cggaaaggac ggggcagggc
    10201 cagttcttgc aggaaaggat agtaggcgct tttcagggtg atttgcagct cgtttttgct
    10261 gagtgctttg acatcaacaa tctggtttgc cagctccagc caggcgtgac gttgacggtt
    10321 atcgagcact gcacggaagt tttctgccgc cgcctctgca tcaaacggtt cgccgttgga
    10381 gaacttcacg tcatcacgca gggtgaaggt ccaggtttta ccatcttctg aatgagtcca
    10441 gctttttgcc agccacggga tcaccgaacc gtctgcctga tatttcacca gcggttcata
    10501 aaccatgctc tgggcgaaca tttggttagg cgtgtaaagg tgcgggttta gtggcccgac
    10561 attcaccggc caggcggtgg tgatttcatc tggtgcagcg gcatggacga taaaagacgc
    10621 acaagccagc agcgcaaata gagtgcggcg gagtgtagag aacatggtaa ccccaatgga
    10681 ttaaaataga tggcggaaat aagtatgacg attttaagta ttcgtcatac tgattacctg
    10741 ttctggatca atagatgggc agtcgggtgg gcggatcagt taactgaatc gatccattgc
    10801 acactgtcgg cggtgagcgt aaagggggta ggggtgcaga tcgccaggct taaattttct
    10861 agctgacaat ggctgactga cagcgaggac tgataggtgc tgtctacgct gacgatttgc
    10921 caggcgcttc cgccacgctg tttaacgatg gcttctttgc gcgtccagat gcgccagaac
    10981 atttccagtt gctgatcagg atgcacggcg tccatctcgg catgttcccc gaggctgaat
    11041 acggcgttcg ccagccagcg ccagttggcg cgcgggcgaa tcacttcgat atcgcagccg
    11101 acttcgcctt catcgctcaa cagcagggcg atatcgtcac cgctatggct taagttgaac
    11161 catagcggcg tttccggcgc gaatgcaggt ttgccttgtt cgccatagat gatctccggt
    11221 agcggggaaa gcgtgtgcga aagcaatgca cgtcccgcca gccagcgttc gcgtcgtgga
    11281 ccttgcggtg cttgctcgcg taaacccggt ggcagaggag ctgcgcttaa ggtcgaaact
    11341 ttccccagaa ctatccgata catatcaggg ccaacgttta atggaaaatg aaagtgcgta
    11401 tcgtaacact tgtcgcctca tcccggtaac cgacttttcg gtctgcctgg ccccagtaaa
    11461 atcgccagtt tgctgccgcc tttggtggtt tccatccaga ttttacacac gctggttaat
    11521 ggcaccgaaa gtagcatccc taccgggccg agcagccatc cccaaattaa caatgaaaga
    11581 aataccacca tggtggacat ccccaggcga tggcccatca tccgtggttc taaaatattg
    11641 ccgatgacca tatggaccac taaaaacaat gcgccgacca gaatacattc gtaaatacca
    11701 ttaaacagca gcacctgaat cattggcggt acggcggaaa ttaccgcacc gatattgggc
    11761 acgtagttga gcaaaaacgc cagtactgcc cacatcagcg caaactgcac ccccatcagc
    11821 tccagcccca gccagacgat tatgcctgtc cataaactga gtagcgtctt taacgccaga
    11881 taatgcgaaa cgcctttaag tgcgcggtgt agtccggcga tgtgaatctg tggattattc
    11941 aacgcgaaac gcattttgta agggacgtgg cgcacttcaa acagcataaa aactacggtc
    12001 atcaccagca aaagcacgct cgccattgcc ccggaaagcc cggtcattag cgccgtggtg
    12061 aaggtaacca ctttttccga gtccatccgc tgtagtattc gctccggcga catatgcaaa
    12121 ttaagaaaag gcaacatctc ctgcaattta aaaagcttgc gcgtcagctc cttattaaac
    12181 ttcggcagca tagagataaa ttcgttaaac gatgccgcca gtacgccgac cagcgcggtg
    12241 agtgcgatca gcatcaccac aaccacaatc gtaatggcaa cggggcgttg tactccccga
    12301 cgaataaacc aggtgacgag cgggttgaga acgatggcaa aaaacagcgc cagtaatagc
    12361 tgcacaatga tatctgccgc tgcgtgaatg cccgcgagga tcactaccag cgaggccaac
    12421 ttgagcagaa tgtgcatgcc cgttttatcg ggttgagggg tttccattgg ggcttccttg
    12481 tgactttttg tattaagtgt agcgggagtc acgccagcaa tattctgatt cttactgctg
    12541 attttcacgt cagcacatgg taaaaatgaa acactgttgt aaaaatgtgg tgatcctcat
    12601 gcccgaaccc gtagccgaac ccgcgctaaa cggattgcgc ctgaatttgc gcattgtctc
    12661 cattgtcatg tttaacttcg ccagctacct caccatcggg ttgccgctcg ctgtattacc
    12721 gggctatgtc catgatgtga tgggctttag cgctttctgg gcaggattgg ttatcagcct
    12781 gcaatatttc gccaccttgc tgagccgtcc tcatgccgga cgttacgccg atttactggg
    12841 acccaaaaag attgtcgtct tcggtttatg cggctgcttt ttgagcggtc tgggatatct
    12901 gacggcagga ttaaccgcca gtctgcccgt catcagcctg ttattactgt gcctggggcg
    12961 cgtaatcctt gggattggac aaagttttgc cggaacggga tcgaccctgt ggggtgttgg
    13021 cgtggttggt tcgctacata tcgggcgggt gatttcgtgg aacggtattg tcacttacgg
    13081 ggcgatggcg atgggtgcgc cgttaggtgt cgtgttttat cactggggcg gattgcaggc
    13141 gttagcgtta atcattatgg gcgtggcgct ggtggccatt ttgttggcga tcccgcgtcc
    13201 gacggtaaaa gccagtaaag gcaaaccgct gccgtttcgt gcggtgcttg ggcgcgtctg
    13261 gctgtacggt atggcgctgg cactggcttc cgccggattt ggcgttatcg ccacctttat
    13321 cacgctgttt tatgacgcta aaggttggga cggtgcggct ttcgcgctga cgctgtttag
    13381 ctgtgcgttt gtcggtacgc gtttgttatt ccctaacggc attaaccgta tcggcggctt
    13441 aaacgtggcg atgatttgct ttagcgttga gataatcggc ctgctactgg ttggcgtggc
    13501 aactatgccg tggatggcga aaatcggcgt cttactggcg ggggcggggt tttcgctggt
    13561 gttccctgca ttgggcgtag tggcggtaaa agcggttccg cagcaaaatc agggggcggc
    13621 gctggcaact tacaccgtat ttatggattt atcgcttggc gtgaccggac cactggctgg
    13681 gctggtgatg agttgggcgg gcgttccggt gatttatctg gcggcggcgg gactggtcgc
    13741 aatcgcgtta ttactgacgt ggcgattaaa aaaacggcct ccggtggaaa tacctgaggc
    13801 cgcctcatca tcttaaaact tactgaataa ccagcgtatt aatgatgttt tctgcggtgg
    13861 tctgtgcttt ttgctgattg tcagctggca gcgtaatttg catggttagc agttgattac
    13921 ccacgttacc cagaataacg gaagagtacg ccgtctggcc tttcgcggag ataatgctgt
    13981 ctaactgctg cattttgtga cctttcagct caatggcttt attggttacc acttgcagct
    14041 gcggatcgcg gctacgttgc tgatcttcca gacgcttcgc cagcaccgcc agatcttctt
    14101 tcggatcatc gcccatgatg acgatgactg ctttctgccc ggtggcgtcg gaccagacat
    14161 gcatgttatt ggcctgcgtt cccagcttac cgctctggtc ggtcatatcc gctggcagcg
    14221 agaaacttaa cttgccatca agtaggttga cgggattccc ggtcgcgtta ctttctgcga
    14281 ccgaaccctg cgccgtagcg ttagtgtctt tatcatcaca ggccgcaagc cccataacca
    14341 gcaggccaat tccgacatat ttaaccagat tgcgcattga cttcttcctt tcgataaacg
    14401 gccataacgg ctcattcatc catcttatca caactctgat aacgaacctt taactcgcct
    14461 gcaaagcgtt gatttcggat ttatctgcca gtcttttcaa cagcatattg agtagaacgc
    14521 catacattgg caggaagaaa acgatactga ttaacacttt gaaacagtaa tcgaccagcg
    14581 cgatttccat ccagtgttca gccataaagg catccgggct acgccagaag gcaatgaaga
    14641 agaaggccag cgtgtcgctg acgttaccga acagtgtgga cgctgtcggt gccagccacc
    14701 agtgacgact ctgacgcagg cggttaaaaa cgtgcacgtc gaggatctgc cccagcgcgt
    14761 aggccatgaa actggcggtg gcgatacggg cgacaaacag gttgaagtgg gcgagcgcgc
    14821 cgaatccttg ccatgacccc atataaaaca gcgatgagat gacgtaggag atcagcaatg
    14881 cggggatcat taccgcaaag atgatgcgac gagccagcgg cgcgccaaaa atacgcacgg
    14941 tcaggtcagt agcgagaaag ataaacggaa agctaaacgc gccccaggtg gtatgaaaac
    15001 caaaaatgga gaccggaagc tgaaccagat agttactgga ggtgatcacc agaagatgaa
    15061 ataacgataa ccagaacaac gccttatagc gttgcgtttg cgagaaaacg ttcatattgt
    15121 acctttttga ttaaccattg gggtgaggga acccaatacg tacgacacgt ctgttacctt
    15181 aacataataa cgtgcttacc cttttttcga gccgccgcat gatactgctt tacgttgaca
    15241 atgcaatggt taattttcac gcaatcgtta acctggtttg cttacggact cacagggcgt
    15301 aaactagcgc cgttttttta agtgatgaga agaaaatgac cgatctcttt tccagccctg
    15361 accacacact cgacgcgctt ggcctgcgct gcccggaacc ggtgatgatg gtgcgcaaaa
    15421 ccgtgcgcaa tatgcagcct ggcgaaacgt tgctgattat cgccgacgat ccggccacta
    15481 cccgcgatat tcctgggttt tgtaccttta tggaacacga actggttgct aaagagacgg
    15541 atggactgcc ttatcgttat ttgattcgta aaggcggttg atgggggaag atgggcttcg
    15601 atgccgcctt ttcccctcac cctaaccctc tccccagagg agcgagggga ccgatcgctc
    15661 tcaatgttgc tgcttgaaac cggtttgtag gccggataag gcgttcaagc tgcatctggc
    15721 gatacggtgc acaatgcctg atgcgacgct gtcgcgtctt atcatgccta cgagttcgat
    15781 gctctgtgta ggccggataa ggtgttcacg ccgcatccgg cattgttgga ccgtagactg
    15841 atagcctctt tatttcctgc gcaataaccg taacgcattc gccgttacca gcaccgtcgc
    15901 acccgtatct gccagcaccg ccagccacag gccggtcatc cctaacagcg tggtgacgag
    15961 gaaaacgcct ttcagcccca gcgcaatagt gatgttctgg cgaatattcg cgtgagtggc
    16021 gcgcgccagt tcaatcattt gcaccaggcc gcgcaggtgg ttatgcgtca acgccgcgtc
    16081 ggcggtttcc agtgcgacat ctgtgccact gcccattgca atcccaatag cggcggcttt
    16141 catcgctggc gcgtcgttaa taccgtcgcc gaccatcgcc agcggcgcat gttgattcag
    16201 ctcggtcacc gctttaactt tatcttccgg caagaggccc gctttaaatt ccagccccag
    16261 ttccccggcg attgccgctg ctgcgcgtgg attatcgcct gtaaggatca cccctttgac
    16321 gcccagcgcg ttcagttcac tgatagcagt tgcagcatcg gcgcgcaggg tatcctgcaa
    16381 cgcaatgaca cccagcacgt catcattacg tactaccagc actaccgttt gcccggcgct
    16441 ttccagttca ttaatcaaac cagcaaatgc atcagcagga tgttttcctg cagcgcatat
    16501 caacacgcgc tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga
    16561 ttgggcggtg ggaatggcga gttcagcaac ctgtgcttcg cgcacaatgg cctgtgccag
    16621 tggatgcgtc gcgccttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat
    16681 acccgttgcc ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt
    16741 atcaaacgcc acctgggtaa cacgacccag ctgctccagc gccgcgccgc ctttaatcaa
    16801 cgccccacga cgcgctgctg ccgccagccc ggaggtaatc gccgcgggcg tggagataac
    16861 taaagcacac gggcaaccaa tcagcagcag cgtcagccct ttataaatcc actcctgcca
    16921 gctggcggca aacagcagcg gcggcaccag cgttaccagc agggcgacgg ccataatcgc
    16981 tggcgtgtaa atacggctga aacggtcgat aaaccgctca ataggtgcgc ggcgctcttc
    17041 ggcttcttca atcagtttca gaatccggtc aatggcgctg gcacccggtt cagacagcac
    17101 ttccagcgtt accagacggt ctacgctggt ggcgcctgca ggaactttat cgcccgtcgc
    17161 gcgctccaca ggaatggatt ctccggtcag ggcactttca tcaaaactgg caaacggtga
    17221 gagcagttta ccgtcggcag gcaaacgccc acctgccgca acttcaatca catcgccagg
    17281 ccgcaggctg tttattgcca cctcttcacg ctcaccgtta cgcagacgcg tggcggtttc
    17341 tggcttcagc gccattaacg cgctaacgcc ctgacgcgcg cggctggcgg cccagccttc
    17401 cagtcgttca ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat
    17461 aaacagcgca ccaatagcgg ctacgctcat taaggtttca atggcgaagt agctgccaga
    17521 tttgatcagc cgtaatgcct gacgagcaat tgggtacagc ccaaccagcg tggtcgcgat
    17581 aaacgccagt tgaccaaacg gatgattgaa ctgctccagc ccccaactga ttgccatcat
    17641 cacgataagc gtaatcagcg gcagattctc tttcaggcgt gattcttgtg gttcgtcgga
    17701 ggcctgttca tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcccg
    17761 gatgtcattg tcggcatcca ccaccagttt ttcggtggcg aacaacacct gtacctgatt
    17821 cacgcctgca agctggcgca cggcattttc cactttgcgc gcacaggcgg cgcagtccat
    17881 accgctgact ttccagctat agcgggtgcc ggagacgttt tcagagagag ttggcgtgct
    17941 ggaacatgcg ccgtcgcagc aacagtcgtt ggtgttctgt accgtagtta gcggtttgaa
    18001 cgcagcaaat tgaggggctt tcttgccgtg attgtcagga gtcgacatgg catcctccgg
    18061 ttaagttttt tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt
    18121 atcagagata cagcgagcgg acgatgagga agtgaccagc aaagtagcag gccgcggcaa
    18181 tggcgttatc cgcacggaag cgtcggcgat agtgactacc cagccagaca aagttactga
    18241 taaacagcaa cgaagcgccg acaaacgcag agagcgccgg agcggtcgga cggaagaacc
    18301 acagttcacc tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc
    18361 aacgatactc ttccagacgc gtccagataa tcgccagtaa cagcgcgccc agaaccagca
    18421 gcaccaacgg cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt
    18481 acagcaggtg cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca
    18541 gggttagcgc atcgcccagc agtgaggcac acagccccgc cagcaccaga tagctaatgg
    18601 cgtcgaacat cggcgcttgc caggccagca acagcaggag aagaagggtt aacggtttaa
    18661 acacccagcg ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa
    18721 gacagacagc gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa
    18781 gcggaatgcc tacggtcttg tttccagttt agtggcgagg gtggacggat gacaacacca
    18841 taactggcag ggtatgctta tttccgcatt ttccgatgag ggataaagat gagtaaacca
    18901 cctcttttct ttattgttat cattggctta atcatcgtcg ccgcatcgtt tcgttttatg
    18961 caacaacgac gggaaaaagc tgataatgat atggcaccgc tccagcaaaa gctggtggtg
    19021 gtgagcaaca agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact
    19081 ccggcaggca ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag
    19141 cagacgtttc gcctcgacgc ccagcagtac cacgccctga cagtgggtga taaaggtacg
    19201 ctgagctata aaggaacgcg ctttgtcagc tttgtaggcg aacaataagg ttatttttta
    19261 actttaaatt tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc
    19321 ttttcggcgg tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagc
    19381 tgcataccat gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg
    19441 cgtgggaagg gatgcaccag gttgaggatt aaaaatcccc aaacacagag cattaacaaa
    19501 cgaccaatat taatcagcat cactttctcc ttgtgcttcg cgttgataca gtcgataagc
    19561 cacctgaccc gccacttttt cccgatgtaa tgaccagttt gccggaacag tgggcagacc
    19621 gttttcgact tcgctttcga cataaatcag ggcttcgtca gccagccagc cgttatcttc
    19681 cagtaaattt atcgtctctt ctaacaagcc acggcggaac ggtggatcga caaacacgat
    19741 attatgcggt gtgccttttt gcgccaggaa tgacatcgcg ttgctgttca ccacgcgtgc
    19801 attgcctgct tttagtgtcg ccagattctt aattaactgc tgagaaaccg cacgatccat
    19861 ctcaatcaac gtggcccccg cagcgtagcg cgataacgct tccagcccca gcgcgccgct
    19921 cccggcgaag caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa
    19981 cgtttcgcgt acgcggtcgg tggtggggcg cagacctgga ctatcaggaa ccgggagttt
    20041 acggcctcgc cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt
    20101 tttcatgata aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt
    20161 aagttaagtg atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct
    20221 gtgccatgaa gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct
    20281 ttttttcctg gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc
    20341 agaatgaaca accagttgta gaagaaatcg ttcaggcgca agagcctgtg aaggcttctg
    20401 aacacgccgt tgaagagcag ccgcaggcgc atactgaagc cgaggcggaa acttttgctg
    20461 ccgacgttgt ggaagttact gaacaggttg ctgaaagtga aaaagcgcag cctgaagcgg
    20521 aagtcgttgc acagccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac
    20581 gtgaagaact gccgttgccg gaagacgtca acgccgaagc ggtttcgcca gaagagtggc
    20641 aggccgaagc ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag
    20701 aaatcaccga cgaagagctg gaagcacagg cgctggcttc cgaagcggca gaagaggcgg
    20761 taatggtggt ttctccggca gaagaagagc agccggtgga agaaatcgct caggagcagg
    20821 aaaaaccgac caaagaaggt ttcttcgcgc gcctgaaacg cagcctgtta aaaaccaaag
    20881 aaaatctcgg ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt
    20941 ttgaagagct ggaagaacag ctgttgatcg ccgatgtggg cgtggaaacc acacgtaaaa
    21001 ttatcaccaa tctgacagaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct
    21061 atggcctgct gaaagaagag atgggcgaga ttctggcgaa agtcgatgag ccgctgaatg
    21121 ttgaaggtaa aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacca
    21181 cgacgattgg taagctggca cgtcagtttg agcagcaggg taaatcggtg atgctggcgg
    21241 cgggcgatac tttccgtgca gcagcggttg aacagcttca ggtctggggt cagcgcaaca
    21301 atattccggt gattgcccag cataccggtg cggattccgc ttctgttatc ttcgacgcca
    21361 ttcaggccgc taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc
    21421 agaacaaatc gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg
    21481 acgttgaagc gccgcatgaa gttatgctga ctattgatgc cagcaccggg cagaatgcgg
    21541 taagccaggc caaactgttc cacgaagccg ttggcttaac cggcatcacg ctaacgaaac
    21601 tggacggcac ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta
    21661 tccgctacat tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact
    21721 ttatagaggc actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa
    21781 ggcttatctc ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga
    21841 gatggcgttt ctgatcggtc attccggcgc agggaaaagt accctcctga agctgatctg
    21901 tgggattgag cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct
    21961 gaaaaaccgt gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca
    22021 tctactgatg gaccgtactg tctatgataa cgtggcgatc ccgctgatta tcgccggtgc
    22081 cagcggtgat gacattcgtc gccgggtgtc ggcggcgctg gataaagtcg ggctactgga
    22141 caaagcgaag aacttcccta ttcagctttc cggcggtgaa caacagcgtg ttggcattgc
    22201 ccgcgcggtg gtgaacaagc ccgcggtact gctggcggac gaaccgactg gtaacctgga
    22261 cgacgcgctg tcggaaggca ttttacgtct gtttgaagag tttaaccgcg ttggagtaac
    22321 cgtattgatg gcaacgcacg acatcaacct gatttcgcga cgttcttatc gcatgctcac
    22381 cctgagcgat ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc
    22441 acattcggca gtttggcggg cgtctggatc gcttccgtaa atcggtcggc ggctctggcg
    22501 acggcggtcg taacgcgccg aaacgcgcga aatcctcgcc aaaaccggta aatcgcaaaa
    22561 ccaacgtttt caacgaacag gtacgctatg ccttccacgg cgcattgcag gatctgaaaa
    22621 gcaaaccgtt cgccacgttt ttaacggtga tggttatcgc catctccctg acgctgccca
    22681 gcgtctgtta tatggtgtac aaaaacgtta accaggcggc gacgcaatat tatccgtcac
    22741 cgcaaatcac tgtttatctg caaaaaacgc tggatgatga cgccgctgcg ggcgtggtgg
    22801 cacagttgca ggccgagcaa ggcgtggaga aagtgaatta tctttctcgt gaagacgcac
    22861 tgggcgagtt ccgtaactgg tctggttttg gtggtgcgct ggatatgctg gaagaaaacc
    22921 cgcttccggc agtagcggtg gtgatcccga aactcgattt tcaggggacg gagtcactga
    22981 atacgctgcg tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca
    23041 gctggtttgc ccgcctggcg gcgttaaccg ggctggtcgg gcgcgtttcg gcgatgatcg
    23101 gcgtgttgat ggtggcagca gtgtttctcg tcatcggcaa cagtgtgcgt ctgagcatct
    23161 ttgctcgccg tgactctatt aacgtgcaga aactgattgg tgcgacggat ggtttcatcc
    23221 tgcgcccgtt cctgtacggt ggcgcactgc tgggattttc tggcgcattg ttgtcattaa
    23281 ttttgtcaga aattctggtg ctgcgattgt catcggcggt tgcggaagtg gcacaggttt
    23341 tcggaacgaa gtttgatatc aatggtttat cgttcgatga atgcctgcta ttgctgctgg
    23401 tatgctcgat gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact
    23461 ttacgcctga ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg
    23521 aaagagaccc cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt
    23581 tattcacagc gttgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga
    23641 taacctcgtt gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata
    23701 tcgattgaga ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc
    23761 aacctggatt cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag
    23821 cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc
    23881 ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca
    23941 caggcggatt tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgttttaac
    24001 ccggaagtgg gtgtgcgcct ggtctccttc gccgttcact ggatcaaagc agagatccac
    24061 gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcaacgcaaa
    24121 ctgttcttca acctgcgtaa aaccaagcag cgtctgggct ggtttaacca ggatgaagtc
    24181 gagatggtgg cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggaatcacgt
    24241 atggcggcac aggacatgac ctttgacctg tcttccgacg acgattccga cagccagccg
    24301 atggctccgg tgctctatct gcaggataaa tcatctaact ttgccgacgg tattgaagat
    24361 gataactggg aagagcaggc ggcaaaccgc ctgaccgatg cgatgcaagg tctggacgag
    24421 cgtagtcagg atatcatccg cgcgcgctgg ctggatgaag acaacaagtc cacgttgcag
    24481 gaactggctg accgttacgg tgtttctgct gagcgtgtgc gccagctgga aaagaacgcg
    24541 atgaaaaaat tgcgcgctgc tattgaagcg taatttccgc tattaagcag agaaccctgg
    24601 atgagagtcc ggggtttttg ttttttgggc ctctacaata atcagtttcc ccctccggca
    24661 aaacgccaat ccccacgcag attgttaata aactgtcaaa atagctattc caatatcata
    24721 aaaatggggt atgttttagc agagtatgct gctaaagcac gggtagctac gtataaaacg
    24781 aaataaagtg ctgcacaaca acatcacaac acacgtaata accagaagaa tggggattct
    24841 caggatgaac ataaagggta aagcgttact ggcaggatgt atcgcgctgg cattcagcaa
    24901 tatggctctg gcagaagata ttaaagtcgc cgtcgtaggc gcaatgtccg gtccggtggc
    24961 gcagtacggt gaccaggagt ttactggcgc agagcaggcg gttgcggata tcaatgctaa
    25021 aggtggtatc aaaggcaaca aactgcaaat cgtaaaatat gacgatgcct gtgacccgaa
    25081 acaggcggtt gcggtggcga acaaagtcgt taacgacggc attaaatacg tgattggtca
    25141 cctctgctct tcatcaacgc agccagcgtc tgacatctac gaagacgaag gcattttaat
    25201 gatcacccca gcggcaaccg caccagagct gaccgcccgt ggctatcagc tgatcctgcg
    25261 caccaccggc ctggactccg accaggggcc gacggcggcg aaatatattc ttgagaaagt
    25321 gaaaccgcaa cgcattgcta tcgttcatga caaacagcag tacggcgaag gtctggcgcg
    25381 tgcagtgcag gacggcctga agaaaggcaa tgctaacgtg gtgttctttg atggcatcac
    25441 cgccggggaa aaagatttct ctaccctggt ggcgcgtctg aagaaagaga atatcgactt
    25501 cgtttactac ggtggttatc acccggaaat ggggcaaatc ctgcgtcagg cacgcgcggc
    25561 agggttgaaa actcagttta tggggccgga aggtgtggct aacgtttcgc tgtctaacat
    25621 tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg aagaactacg atcaggttcc
    25681 ggcgaacaaa cccattgttg atgcgatcaa agcgaaaaaa caggacccaa gcggcgcatt
    25741 cgtctggacc acctacgccg cgctgcaatc tttgcaggcg ggcctgaatc agtctgacga
    25801 tccggcagaa atcgccaaat acctgaaagc gaactccgtg gataccgtga tgggaccgct
    25861 gacctgggat gagaaaggcg atctgaaagg ctttgagttc ggcgtatttg actggcacgc
    25921 taacggcacg gcgaccgatg cgaagtaatc attaatcggc aactttgggt tgccgccaaa
    25981 ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg taaacgcctt atccggccta
    26041 caagattcaa agaaatcagt aaattgcagc acacattgta ggcctgataa gcgtagcgca
    26101 tcaggcaata tcacttgtga aatcaaactt gacgattaac gcttctccca gccgccctgt
    26161 tgtgccgtaa atcccagtgc ctgcataaac gctgtcatca cgccgcgatc ttccacgcct
    26221 gcatccgcca tccaccagca tgaaacgcca ggattgttac gcaaaacctc ttccagcaga
    26281 tattgcccca caccgcgacg gcgggtgact tccctcacac gcagggaatc cagtgccccc
    26341 tcggtgccgc ttaaggttac ccgcacggcc gcgagaaggc gctcgttaaa acgcgcggcg
    26401 tagatacggt ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt
    26461 tgcaggtcaa tccggtcctg gtcgctaaag ttttctaatc gaatgatggt cagcttcatg
    26521 gggaacccgt gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct
    26581 ttctcttttt taattctttt ggcaggtgat taatttttta acagcaataa ttacaaagtt
    26641 aaaacattag agaatgaaaa atacccagca taatcccctg aatggtagtg aattattccg
    26701 ccctttgtac cgttatttta tgctgac
//
LOCUS       DAZFRG010000039        26725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFRG010000039 DAZFRG010000000
VERSION     DAZFRG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.039"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..370)
                     /gene="yafO"
                     /locus_tag="ACHKUC_001313"
     CDS             complement(<1..370)
                     /gene="yafO"
                     /locus_tag="ACHKUC_001313"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO6617598.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGK"
     gene            complement(373..666)
                     /gene="yafN"
                     /locus_tag="ACHKUC_001314"
     CDS             complement(373..666)
                     /gene="yafN"
                     /locus_tag="ACHKUC_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO6617599.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(718..1773)
                     /gene="dinB"
                     /locus_tag="ACHKUC_001315"
     CDS             complement(718..1773)
                     /gene="dinB"
                     /locus_tag="ACHKUC_001315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6617600.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1844..2767)
                     /gene="lafU"
                     /locus_tag="ACHKUC_001316"
     CDS             complement(1844..2767)
                     /gene="lafU"
                     /locus_tag="ACHKUC_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6617601.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHLPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            complement(2770..3633)
                     /gene="motA"
                     /locus_tag="ACHKUC_001317"
     CDS             complement(2770..3633)
                     /gene="motA"
                     /locus_tag="ACHKUC_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6617602.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(3646..4362)
                     /locus_tag="ACHKUC_001318"
     CDS             complement(3646..4362)
                     /locus_tag="ACHKUC_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO6617603.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(4382..4849)
                     /locus_tag="ACHKUC_001319"
     CDS             complement(4382..4849)
                     /locus_tag="ACHKUC_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO6617604.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAVARTEA
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(4857..5918)
                     /locus_tag="ACHKUC_001320"
     CDS             complement(4857..5918)
                     /locus_tag="ACHKUC_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6617605.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQHQATRPQENDPVAMQALMALLLPQPAAPHQDTPEPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(5915..6226)
                     /locus_tag="ACHKUC_001321"
     CDS             complement(5915..6226)
                     /locus_tag="ACHKUC_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO6617606.1"
                     /translation="MEDKCRQLFALVEAMNEALDKQRWRRLPALHQQVMRQFHDYATA
                     ETDATQLCAVKAQLCAAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(6231..6623)
                     /gene="fliS"
                     /locus_tag="ACHKUC_001322"
     CDS             complement(6231..6623)
                     /gene="fliS"
                     /locus_tag="ACHKUC_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6617607.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(6646..7962)
                     /gene="fliD"
                     /locus_tag="ACHKUC_001323"
     CDS             complement(6646..7962)
                     /gene="fliD"
                     /locus_tag="ACHKUC_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6617608.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAASSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQGAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFSDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(8608..9522)
                     /gene="lafA"
                     /locus_tag="ACHKUC_001324"
     CDS             complement(8608..9522)
                     /gene="lafA"
                     /locus_tag="ACHKUC_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO6617609.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            10008..10850
                     /locus_tag="ACHKUC_001325"
     CDS             10008..10850
                     /locus_tag="ACHKUC_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO6617610.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEAQIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(10909..11886)
                     /locus_tag="ACHKUC_001326"
     CDS             complement(10909..11886)
                     /locus_tag="ACHKUC_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO6617611.1"
                     /translation="MQVGLTSSSLATGGALSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRQLQPVLQGEARVTFHSLDLANLVHNPTPGTRMFSVTDGRQTQLSA
                     VMLYEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQADRLAQSLQQGGAGISQMLENINQQRAQMAVQQEKARQLIDGMS
                     RFPQTESAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(11903..12832)
                     /gene="flgL"
                     /locus_tag="ACHKUC_001327"
     CDS             complement(11903..12832)
                     /gene="flgL"
                     /locus_tag="ACHKUC_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6617612.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFDSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(12847..14223)
                     /gene="flgK"
                     /locus_tag="ACHKUC_001328"
     CDS             complement(12847..14223)
                     /gene="flgK"
                     /locus_tag="ACHKUC_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6617613.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTASMTLTFAGTISTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(14413..14712)
                     /locus_tag="ACHKUC_001329"
     CDS             complement(14413..14712)
                     /locus_tag="ACHKUC_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO6617614.1"
                     /translation="MKINGSGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPRHK"
     gene            complement(14712..15812)
                     /locus_tag="ACHKUC_001330"
     CDS             complement(14712..15812)
                     /locus_tag="ACHKUC_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6617615.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVTLHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(15827..16492)
                     /gene="flgH"
                     /locus_tag="ACHKUC_001331"
     CDS             complement(15827..16492)
                     /gene="flgH"
                     /locus_tag="ACHKUC_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6617616.1"
                     /translation="MKNYIWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(16563..17348)
                     /gene="flgG"
                     /locus_tag="ACHKUC_001332"
     CDS             complement(16563..17348)
                     /gene="flgG"
                     /locus_tag="ACHKUC_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6617617.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(17427..18164)
                     /locus_tag="ACHKUC_001333"
     CDS             complement(17427..18164)
                     /locus_tag="ACHKUC_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO6617618.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGVSGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(18164..19366)
                     /gene="flgE"
                     /locus_tag="ACHKUC_001334"
     CDS             complement(18164..19366)
                     /gene="flgE"
                     /locus_tag="ACHKUC_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6617619.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     DAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPNTPQTIEFQTDAAAPIDLTVDYSSCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(19630..20343)
                     /locus_tag="ACHKUC_001335"
     CDS             complement(19630..20343)
                     /locus_tag="ACHKUC_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO6617620.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTTSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(20343..20774)
                     /gene="flgC"
                     /locus_tag="ACHKUC_001336"
     CDS             complement(20343..20774)
                     /gene="flgC"
                     /locus_tag="ACHKUC_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6617621.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(20777..21112)
                     /locus_tag="ACHKUC_001337"
     CDS             complement(20777..21112)
                     /locus_tag="ACHKUC_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO6617622.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            21194..21931
                     /gene="flgA"
                     /locus_tag="ACHKUC_001338"
     CDS             21194..21931
                     /gene="flgA"
                     /locus_tag="ACHKUC_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6617623.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELRPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNTSSQRVISAMVAESGVVTTVSAE"
     gene            22012..22290
                     /gene="flgM"
                     /locus_tag="ACHKUC_001339"
     CDS             22012..22290
                     /gene="flgM"
                     /locus_tag="ACHKUC_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6617624.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            22303..22731
                     /gene="flgN"
                     /locus_tag="ACHKUC_001340"
     CDS             22303..22731
                     /gene="flgN"
                     /locus_tag="ACHKUC_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO6617625.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEDL
                     LAVNDEITQHYEQLHGHSYQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            22859..23791
                     /locus_tag="ACHKUC_001341"
     CDS             22859..23791
                     /locus_tag="ACHKUC_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO6617626.1"
                     /translation="MSFIECYEPEYVRNFMTQYPDSHLYVRKVWKNEIRQSLALTDIE
                     SCKAVLNDARAFDLQLTYRPVEDNAAELSARDAIVNQAILSTLTLPDLTPELSLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHSQKGTLQAQFAQLPPVPQLARQLMTLLG
                     SFTFDWSILPESPRKASLPLQVTLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQTDGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENAVLIRQQIQAECSADPLLYAFSLLSC"
     gene            23838..26276
                     /locus_tag="ACHKUC_001342"
     CDS             23838..26276
                     /locus_tag="ACHKUC_001342"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6617627.1"
                     /translation="MQTLIKTLATGRVKPFVSVVTPTWNRGTFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPGSFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTNNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAALTDIVSDPLLRNAYLGLHNATHYQAIQHQAIDQIVLLNLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALHLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLNTLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYYTPALSGLSPLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWGAVHARLTEIRHHYCDAFMDGK
                     AGERAALVIVEALG"
BASE COUNT         5811 a         7453 c         6979 g         6482 t
ORIGIN      
        1 ttttcccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt tcaggtttca
       61 gaatggcaat gagcaaccat gcttgctcat caaacgcgcc ctgacaatat accaaatgcg
      121 ctgcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta ttcacatttg
      181 ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac gagtcgtcgt
      241 agagtgcatc acgaccaaat atatctggca aaacaccgtc acgcttatag gaaataaaat
      301 ccgccgttaa cgcttcaagt tcctctgctg taagttgcag gcgaataagt tttgttttga
      361 atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat cattaagata
      421 ttgttcagcg cggcgtgtaa tttcctctaa tcttgcagca ctcggacgga agcgcgcctt
      481 tatgggcttt ttctcctctt gttctgcaag catgtccatt aatgcttcaa atgcgctggc
      541 acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaacaggtt gatcaataaa
      601 gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag cgagaattcg
      661 atgcatacag tgatctcctc ataataatgc acaccagaat atacaaaata gtatacatca
      721 taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat gcagccccac
      781 cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg tggcgattag
      841 atcagattta ttcagccgcg gccagacgtg ttcctgggtg gtttgctgaa aatcgtcgaa
      901 ctttaatttc accccctggc gggcaatcag caaatcaggt tttacctttg ccaaacgacg
      961 ctccagttca gggtaaagcc gctcgatgat cgcttcacat tcagaccagt gatgaatatc
     1021 ttccgccatc gtacgttcta caccgacgga ttttcgcaac ctttcgctgt taacgtcgcg
     1081 ctcgtcaatg ccctgactac gctcccacaa aatgcggcca aatttgccaa aacgtttgag
     1141 cagagtcacc agatcacact tttgcacatc accgcaggtt cgtagcccca tcgcttccag
     1201 tttagccgct gagactttgc cgacgccggg aatttttgcc agcggtaagg tttgcagaaa
     1261 tgccggaacc tcagccggag taatcacaaa ctgaccgttg ggtttattca tgtcggaggc
     1321 gattttggcg agaaacttga ccggcgcaac gcccgcagac gccgtcagtt gcagctcgtt
     1381 gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc aatggacgct
     1441 atcggtgaca tcgagatagg cttcatccag tgataacggt tcaatgcgcg aggtatagcg
     1501 cgagaaaatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc gccctggaag
     1561 caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc tacgtacgcc
     1621 aaatttacgc gcgggataat tggcggtgct gattaccccc cgtcgttcgc ggctgccgcc
     1681 aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg ctgcgaaaaa
     1741 gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac tggtaaagta
     1801 tacagtgatt tcagggtttg agaaatgcgt aaagattcag catttaacgc gctcgctgcg
     1861 aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta tccccatgct
     1921 gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata atctcaatgc
     1981 gccggttgcc cgcgctttgc ggatttttgg catccagcag catctggtcc gccattgcgc
     2041 ttacctgcat cactttattt tccggcattc cggcctcttc cagcacccga cgagccgaaa
     2101 gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag gccatcgcat
     2161 cggtatgccc ggtaataata attttgttat cgagcgagtc gaacactggc gcaagctcca
     2221 ccagcaaagt tttaaagaac ggcataatct tcgcgctgcc gcgttcaaac atattgcggt
     2281 tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc tccagattcg
     2341 cttccatatg cgcatcacgg gcgatggtat tgatactggt cgccagctcg ccaagctcgg
     2401 tggcggattt cttcttcaac agcgccgttt tctcgttgac gtcgcgcgct tttttctccg
     2461 tctcttccgg caccgccacg gtggcaggtt ttggcagatg agacgggctg attttattta
     2521 acggcgacaa cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg ataatgcttt
     2581 cacgctcgga tttactgacg ctgttgacaa tccacagcgt cataaacagc gccatcattg
     2641 ccaaagtaaa gtcggcgaac gccaccttcc acgccccgcc gtggtgcccg gcatgattct
     2701 ttttgatctg ccgccggacg atggtggttt ttgccccacg ctcgcggcgg ttgcccgtct
     2761 ttctcatgct tattcttgct ccagcatcgc attcacccac gcgtcgaggt tggcgaacgt
     2821 cggtttggac gccagatgca gcaacttacg cccggcgtcc accgccaaca gcgtcggttt
     2881 gccgcccgcc tgggccacca gcacggtacg cacacactcc agcagcgaat gttccgcccg
     2941 cgcctgctgt tccatggcgt tggcgagcgg gtccatcagg cagtagcaga taaagacgcc
     3001 taaaaacgtt cccaccagcg ctgccgccac tttcaggcca atcagggcga tagagccgtc
     3061 aatcgactgc atggtgatga tgatgccgag cactgctgca caaataccaa atcccggcat
     3121 cgcttcggca gtacgttgca gcgagcgcga cggcgttaac agggactctt ccgcggtgtc
     3181 cagttcctga tcgagaatgc cttccagctc atgggcgtcg attttaccca tcgccatcag
     3241 gcgaaagtta tcagcaataa aggtcaccag ccgcttctgg gttaacacca gcgggtattt
     3301 ctggaaaatg gtgctctctt ccggctgctc aatatgctga tccaacatcc gcagaccgcc
     3361 gttttgcacc atttcgagca actcatacag gcacattaat agctgacgct gaaactccgg
     3421 cccgagctgt tttttactga tcacgccttt aatttgatgg gcaatctctt tcagcacatg
     3481 tttcggattc ccaataatca tcgccccgaa accagcaccc aaaataatga tcatttccgc
     3541 cggttgccag atagccacta attgcccgcc cgcttcaaaa taaccgccaa atacgcagcc
     3601 gagcaccacc agtaaaccta atattttttg catgatttct tcactttaat tcggatagac
     3661 aaaatcacga attttctgcg cgatctgttt attcagttga caaattcggg cctcggttag
     3721 ccctaatacc agcgcaatct ctttgagatt catctcatgc tggtaataca tggagaggat
     3781 cagttgctct ttttcgctta attgtgaaag cgcgtgttgc aacatttgct gggtgacaaa
     3841 ctgatcttcc agttcccggc cagcaagcgg aatgcctggc ccttcgccgt tcagcagttc
     3901 gtccaggctg gcgagcgttt ccgccccttc cagttgctga tattcgagat aatcttcatg
     3961 cgagatccct tcctcagcca gctcctgcca ctccggttcg tgccccagtt ttttacgcgt
     4021 ttcgcgaatc aggtctttca tctgatgata tttctgtcgc agctggcgcg gtcgccagtc
     4081 gagagcgcgc agctcatcca ggatcgcacc gcgaatacgg tgcacggcat agcccgcaaa
     4141 accttcgtcc ggcagcccat agcgacgaat ggcgttcaac agccccatta acgccgtttg
     4201 ttccatatcc tgacggtcaa taatgcaatt acactgcggg gcaagttgcc gcaccacttt
     4261 acgcaccaac ggtaaatagg cctgcaagta gtggctctcc tgctgcggag ttaataccag
     4321 cgtggcagca aattcttctg actcgaatac atcctgtagc atgtttatat ccgcctggtg
     4381 attactggaa gaccactttt ttcaccagca aatccttata cggtgcggtc attttgcgac
     4441 gctgaagatc tttctttaat gcgtcagaaa tcagtttgcg gatggcggat attttcatgc
     4501 cacgcaaatc ttcaaatttc atttccgtca ggcattcaac cgcaatactt tgatacagcg
     4561 gttcctgatg ctttattttt tccgcctctt tgtcactggc aaccaccatc actaattccg
     4621 ccgacaaata atgatccgcg ccgttgttgt catgcagcgt gacaatggtt tccggcagcg
     4681 tcacaaacac gcttttgctt tcatccaggg tttccaccgc ttccgtccgc gcaacggcag
     4741 tcggttttcc ttttccggca taatgatgcc agacacccca tccagctccg gcccccacca
     4801 ccaacgccag aacggcgctg accacgcccg ccaccacgat tttcttcata ttgcctttac
     4861 actttgatca aaacggattc gttctgttga aattgaggcg catcatcaag ctgtaaggcg
     4921 gcactcacct cttcctgctg atggcccgac tgttgttgct gctgcgactg cccttcggag
     4981 gaaacctgta cgttcacctc cataaaattt tgcgccgtca gatgctggcg gagatcgtcg
     5041 ctaaactgct gcaacgcgcg gcagacttca ctttggttcg cgccgatgtg caccatcagt
     5101 ttgccgttgt cgagttggac ggcgatctca agcttgccaa gcgacggggg atcgagacga
     5161 atggtggaga tctgctgttg tttgttgagc tgaaagtgga tctgatcttt caacagcgtc
     5221 gtcagttttt cgcccagttc gtccatcgaa agcgtggctg gtttggcgac aatctcctga
     5281 tgatcgctga ccaactctgg cgtcttaacc tgcaacaccg cacgcgcaat aaccggttcg
     5341 ctgtccggca ctttttccgt tttacgctcg acctgcgggc gcgcggtcac acgggttagt
     5401 tcaggtttcg ggcgagctgg cggtgttgag acgcgcggct gcgtcggcgc aatggcatgt
     5461 aaatcttcac tggcgtaagt cgccagtctg gcctgctgtt cgggtttcgc ctgcggcaac
     5521 tgaccgatca gctcctgcaa ctgcggcggc aacggcgtga gttcctgctg ctgcgtcggg
     5581 cgttgcggcg cgttttgcac caccgctttc gtcaattgct ggataaccgg cgatgttgcg
     5641 acgtttcgcg gctccggcgt gtcctgatgc ggcgcggcag gttgcggaag taacagcgcc
     5701 attagcgcct gcatcgccac cggatcgttc tcctgcgggc gcgtcgcctg atgctgactg
     5761 gttttagcgc tatgaacgcg ctccgccacc gccgccaccg ccattttcgg cagggtaaac
     5821 gcgggcgcgt tttcaccgct caccggcggt aaaatatccg ctttcaacga cgcggtttcc
     5881 gcaagtgttc cgagcgtggc aagcaatgcc ggattcatga cttctccgaa agtaaattaa
     5941 caattgagta ggccagcacg ccttcctgat ggcgctgatg ctgttccata cgcgccttca
     6001 acacttctgc acgctgctcg cgtcgtttaa tcagggcgtt aaacgcggca cacagttgcg
     6061 ccttcaccgc acacaactgc gtcgcatcgg tttccgccgt cgcataatcg tgaaactggc
     6121 gcatcacctg ctgatgcagg gccggaaggc gtcgccagcg ttgcttgtcg agggcttcgt
     6181 tcatcgcctc aaccagcgcg aaaagttgtc tgcatttatc ttccattgcg ttaacccagc
     6241 ttgcccgaaa gcccttccca gccttcgcgc agattgctta aaatcagcat cacttcatcg
     6301 attttctctg ccgacagttc gccactggct tcatacaagc gatacacgca gtggtcatag
     6361 aggcgagaaa gattcaccac cagctcgcca ccggtttcaa actccagcga actggtgagc
     6421 gcattgagaa tgtcgatgca tttattgatg ctctgggctt tcttctcgaa acgccgccct
     6481 tcaatgtggc ttctggcgcg ttccagctcg tccatcaggc cagaaaacag caccagcacc
     6541 agttcaagag gcgatgccgc agcggtacgc gccgcgagat cgatctcttt gtattgcgaa
     6601 taaccgtcct gtacttggta catctttaag gatattcctt ctgaattacg cgaatgccgc
     6661 catactggct ttcatggtgt acacctcaac caacgtattg gtgtactcct caagataacg
     6721 gtcgtagtta gcgttatagg tatcggtaag ctgatcgcct tcgtcctgaa ttttgctcat
     6781 ctgatcatca atgttctgct ggcgcagggt gatgatgccg ttgctggagt ccgtgtaggt
     6841 attaatcagg tcgtccatct gcgccaccat gctgttgtcg ccaacgaaaa tagatgtcag
     6901 accgtcagga tttttcgcca tttcgtcgct gaactggtcg gagtcaatct gtaagtggcc
     6961 gtgggaatcg agggtgatgc catagtcaac aatcgacacg ccgttgtagc tggcatgggc
     7021 gatatcatcg agctggtttg ccagtgagct gagtccagca tcccccgcaa agaccccggc
     7081 gctactgctg tcatcaccgt gagtggttag cgaatcaacc gtatcaatca aggtgttata
     7141 ggcatcgaca aaagtctgta ctttctcctg gctggcgctg gaatcttcgc tgatgttaaa
     7201 agtggtgaga tcgctgtcag aatcgctgac ttcggtaaaa gtcatggtga cgccggggat
     7261 cacatcatca aacgtgttgc tgctgttggt gatagccggt ccgctggcgc taccgagatg
     7321 aataatcgcg tcctgagcgg aggaaacatc cgtggcaacg ggcgcactgg tgctatcgtt
     7381 gctggcatca tgccccgtta cgctcaccga aaacgcgccc tgcgccccgg tgctatcgga
     7441 ggtgagcata atcgtggtgg tgccgtcggt ttttaccagt gccgccgaca cgcccggatt
     7501 gtcatcggag tcgttaatcg cattcaccag ttcgctggcg tcgataaaac cgtcgccgtc
     7561 accgttctga tccgccgccg acagatcgat atccatagtg ctgtcgccca tcgtcagttc
     7621 aaaagtaccg gtagcggaaa aggcgtcgtc gcccatactg aacgtggttt gctgcccctg
     7681 cgccagttgc tcaacgaaaa acgagtagct tcccgcctgc gcctgggaat tggcggaaac
     7741 ggtcgccgag tcattattgc tgctggcggc aaaagtcacc gggccgtcgg tatcgctgtt
     7801 cagcgcgtca acggcgctct ggaaatcgct caacgccgag ctgagcgagt ccaggccgct
     7861 gctttcagca tccagctccg tttgcttttc ctgcaagttt gccgcctggg tggcaacatc
     7921 cgcatacgcg atttcttgcg caatggttct tgggttgatc attgatgact ccggataaca
     7981 atgtcaggtt attggcaaaa ggcgtgccaa tattttttat gtgaataatc aatagattga
     8041 aaaacaggcg gaaatgacgt ttccggtctg gcggaaaaaa gcctgccgca gaaagcacaa
     8101 caccgcccgc aggcggtgtt gttcgtggcc ggataaggcg tttacgccgc atccggctat
     8161 cggtgcacag tgcctgatgc aacgctgacg cgtcttatca ggcctacggg tccgcgcctg
     8221 tttttatgcg tgtgcccatt tgtaggccgg ataaggcgtt cacgccgcat ccggctatcg
     8281 gtgcacaacg cctgatgcga cgctgacgcg tcttatcagg cctacgggtc cacgcctgtt
     8341 tttatgcgtg tgcccatttg taggccggat aaggcgttta cgccgcatcc ggctatcggt
     8401 gcacagtgcc tgatgcaacg ctgacgcgtc ttatcaggcc tacgggtcca tgcctgtttt
     8461 tatgcgtgtg cccgtttgta ggccggataa ggcgttcacg ccgcatccgg caatcggtgc
     8521 acaacgcctg atgcgacgct gacgcgtctt atcaggccta cgagtccgca catattttta
     8581 tgcttgtgcc cgtttgtcgg cccgaactta ctgcagcagg ctggaaacca tgctggacat
     8641 gctgttggac tgtttcagca tggtgatacc ggtctgcatc agcacctgtt gtttggtcat
     8701 attggacgct tccgtcgcgt aatcggtatc catgatgttg ccgatagcca cttcggtatt
     8761 gtcctgcatg ctggtcaggt tgttggcggt atcattcaga cggttgatag acgcacccag
     8821 tttggactgg atctgcgaaa cgtcatccat cgcggtagaa atgctggtga tcatctggtt
     8881 agccgagcca gacgcagtca gttcagtacc gccagaaacg cctgcggtgc cagtggtgtc
     8941 tgcctgatcc gcactaaagg agttgctgat agcagaaagg tcagtcacca gcgtgttcag
     9001 ttggcttgaa atattcacgg tcatggtgtc ggaagattcc gcgcccacct ggaaagttac
     9061 tgcgctctgg aacagaccgt cagtaccggt gttgctggtg ccggaaacac cgaacaggtt
     9121 ggtgccgccg taagtagtgt tttgcagcat gtcagacatc tgctggccca gttcgtcgta
     9181 ttcgtcctgc atcgcctgca ggtcatcgtc gctgtaggtg ccgttcgctg cctgagttga
     9241 caggtctttc atacgaccca gcacgtcaga catttcgtca aacatgctgt cggcggtttg
     9301 cagcatcgcg gtggcatcat tgatgttgct cagcgccacg cccataccgc tggactgtgc
     9361 cgtcagacgg ttagcaattt gtttgcctgc ggcgtcatcg gcagaagagt taatccggtt
     9421 acctgtcgcc aggcgttcca tagaagtaga cagagaagag ctgcttttgc tgattgcatt
     9481 aacggcggcc attgaggcgt tattggtgtt aatagataac atgatactgc tccttgggat
     9541 taatcgtttt cgtttcgata ccctgtaaaa acgacacctc gggagcaaac ttaaaaccgc
     9601 cgttaaaaaa atttcactgc ggtttatctg atgccacttt tagctaaaaa aagcagagtt
     9661 taaattcaga taaaaagatt attagcaata tctgccacat ggccaggtat caaaatatgt
     9721 ctttaacaaa tgcttgatat agttaagatt gtcctcaatt aacgcgtttt ctcatcaatg
     9781 tactgatgcc aaaatttaca ttgcgttgca aatagtaccc atttggctat tgtacctgtc
     9841 ctgtcatttc gttacatttc cacgccatat agtgattaag aataatccta ttaccataaa
     9901 atattaatta tcacgcagaa aaatcatctt ctcagggacg tataagactg tgctttatgc
     9961 aaagcactat caggaaacat ttatcccttg ctaaggtaaa ctcaactatg agtattatta
    10021 tcaacaactg gcggatggac ccgtcgctta atgccttaat acattgcgaa acgggtgaaa
    10081 cacgtcgtct cggcgaatac cattttattt tgctggaaac attagcaaaa aatgccgatg
    10141 tggttttatc gcgatcttat ttatgtgccg aagtatggaa gaaccgtatt gtcggcggta
    10201 acagcctgcc gacggcgatc catgcgttgc gcgttgctat cgatgatgat ggcaaacaac
    10261 agaacattat taaaacaatt ccgaagaaag ggtacctgtg caataaagag tatgtgtctt
    10321 ttccggagtc atcgcctgca gaagcgcaga ttattaccga ccagatacag aaaacaacgc
    10381 cagaagagat atcttcgaca acaccgcctg tacctgttag aaaaaaacat aaaggactga
    10441 tggggctggc gttggccgcg gctgttatat tggttggctc aaccgtgggg tattcacact
    10501 tgaaaagtac acctgacgcc ccgcaactgg taaaagagtc aattaacagt ccaagaataa
    10561 aaatatttca tcttagttct ggaaaggaaa ataattccgt accgttactg tcacaaatcc
    10621 tcgcaccagg aaaagacaaa ctcgataatt tattgtcagc gcataatatg acaatgacaa
    10681 cctattataa atatgttcgt aaccgactgg aaagcgatat tgttctgcgt aaccagtgca
    10741 acggcagctg gcaattaacc tttaatgtgg aaggttggca gaacagcgat attaatagcg
    10801 cgatgtatca gaatttagag aagttgttaa atactgtgca gaaatgctga caagtgccaa
    10861 tatatgccgg atgcgacgct aaacgcgtct tatccggcca accgataatt accccaataa
    10921 attcctcacc gtctggctgg aaattcgcgc ctgaccgttc accgcctgca ataaaacctg
    10981 atagttatga ttggcgctat ccagcacgcc gccgagattt tccgacgcct gcacggcgct
    11041 ttccgtttgc gggaagcggg acatgccgtc gatcaactgc cgcgcctttt cctgctgcac
    11101 cgccatttgc gcgcgctgct gattaatgtt ttctagcatt tgagaaattc ccgccccgcc
    11161 ttgttgcagg ctttgcgcta agcggtccgc ctgtgacggt tcagcaaagg ttttcagcgg
    11221 cataaacgcc gacttatcgc catcggtgcg cacgcttaag gtgcgctcga tgtctggcca
    11281 ctgtttttcg gtggtggaga agctgatgcc atccgcttgc tgatgcagtt gcacacccac
    11341 gcggcgcaga gcgttggtca gccgcgtctg atactgcgcg gcgctgtcat cctcatacag
    11401 catcaccgcc gaaagctggg tctgccgccc gtcagtgaca ctgaacatgc gcgttcccgg
    11461 cgtgggattg tgtaccagat tcgccagatc cagcgagtgg aaggtcaccc gcgcttcgcc
    11521 ctgcaacact ggttgtaact ggcggtccac cgcgccgccg gaaagcgcgg cgcgtttgtc
    11581 cagcatctgc atcagcgccg tactctgtgc ctgcccgccc ttacgctggc tatgacgata
    11641 atcgagcaac tgctgttcca gttgcccgag atagtgatcc gcctgctgca acgtggtgag
    11701 ctggtcgtta agctgtacgc tgtaacgctg cgggcgagtg gtaatcagcg gcgaggccgg
    11761 atattcactc gccgtcgccg ggagtttttg ccgcaccgcc tgagtgggcg caacggttga
    11821 cgacgatacc gcagcgctaa gtgcaccgcc ggtggcgagt gatgaagatg taagtccgac
    11881 ctgcatactg ttcctgaaag agttagagaa cgctgaacaa attcaactca ctcactttcg
    11941 agtaagtctt gagggtagct tccattgcca cttccagtcc ggtaaaggtg atcgacgctg
    12001 ggccgtaatc gagatcctgc aatgaaccaa tcaattcatc attagatgtc gagatatcgg
    12061 tctgtgcatc gctcaacatc gacatggtgt tttgtgtctc gccgagcgac gcaatcgagg
    12121 cgttgaggtc gtccgaggcg gtgtctacca catcgacggc gttctgaata tcgctctgca
    12181 cctgcggatc tgccggatcg acgtccgggt tttgcagttc ctgcgacaag gaattaagcg
    12241 tgttaagcac gtctaaatta ctgtcgaaga aatcgctcgc cgccacgttg gtatccactt
    12301 ccacgccgtt cgacaccgta gtctggcgat ggtcgctgtt gccctgatag ctatagctgt
    12361 cggtgacgcc atcgccatca tcgtctaccg ccacaatcgg cggctgatcg ttgatggtgc
    12421 cgccaaagac gtaatgcccg tcttcatcct gatagttcag cgccgcgacc attgattcgg
    12481 tgagcgactc aatatcctgg ccgaggcttg ccagagaatc ggcggtattg gtgccattcg
    12541 ccgcttcaag caggtcgtcg cgtaccgcca gcaggctgtt gttgacgcca tcaagaatag
    12601 actcctgctg gcttaagccc gccgacgcgg agtcgatatt gctctgatac tgttcaatcg
    12661 ccgactgctg gcggtttaac tgggtgatgc gcgtggcggc gatcgggtca tccgacggct
    12721 gcaaaatttc cttgccggtt gccatctgct cgaccacatg cgccagagag ccggagagat
    12781 tattaaaact ttgcgtcatc gagacgtagg tttgttgggt ggttactcgc attttcgcgc
    12841 tcccgcttag ctgcacattt ccagcaccga atcgaaaatc tcggccccgg cggaaatgac
    12901 tttcagatta gcttcataaa tttgttgata ggtgatcagg ttcaccgcct cttcgtccat
    12961 gctgacgccg ctgacgctgc tctgctggtt ttgcgctgcg gaatagacat tggacgcggc
    13021 atcgacttcc gtctggtttt gctgactgta aatgccgata ttgctgatga ttgacgagca
    13081 cgcctgccct accgtcacgc tgccgaggtt ggctatttcc agcggttcgg tggagatatt
    13141 gatcagcgcc tgcaggttgt cgctgttgcc gctttcatcc ggtgaactgg aaaacgccag
    13201 ctcatcggca gtgatatccg ggttaacggt cagtgggccg tcggcattgc tggcgtcata
    13261 aataaacagt ggctcgccgg ggttaccgtt gagatcgtag ccctgcgcca gctggttgtt
    13321 gaccgcatcg gcgaactgcg acgccatgct gttgatggtg tcggtcagcg gcgtcagcac
    13381 atcgttttga taatcaaaca gtgcgcctaa tgaaccgcca gtgtcggtgg tcatcgtcga
    13441 gatggttccg gcgaaggtca gcgtcatact tgctgtacca tcggcgttgg tttccagcgc
    13501 gatggtcgag ctttgctgcc cgctcaccag cggctgaccg tttttcaggg tgacgttgta
    13561 gttgccctgg tcgtcaatat tgacctgcac gtccatcatt ccgctcagtt cttccaccat
    13621 ctgatcgcgg gcgtcgtaca gcgcggaggc gttatcgccg ttggcttcgg cctgagcgat
    13681 ttgctggtta tagctggcga tgccgctggt cagcgtattg atttgcgata ccatcgcctg
    13741 ctgctgactg atgatttccg tgctttgcga gtcgatgtaa tccagcgtgt tgtcgatgcg
    13801 caaggacagc gccccggctt cgctgatcac ctgctcgcgc agggcggaat catcggggct
    13861 ggtggtcgct tcgttaaggg cagcgaaaaa gttatcgaag ccgccgctca gactgctgtt
    13921 atcgtcgctc agtaccgctt ccagttgtgt gagataccct tgttgagtgc tgtaatagcc
    13981 gtaatcgctg gcggcatacc atacctgatt aacctgatac tggttggaga cgcggcgaat
    14041 actgtcgacc tgcacgccgt taccggcgct gttcggcgaa ccaccgctgg caccgatggt
    14101 gctgatctcc gccacctggc gggtgtagcc cgtggtcatg gcgttagcgg tgttttgcgc
    14161 cgtgacgttc agctccacct gcgccgttga tgcgccgctg tagccgatgt taatcatgtc
    14221 catgttgggt ccttaccggt aaatcgtgcc ggttaaaagc ggcgtgaagt tgggggttct
    14281 taagttccgt aaaccgggtg ccaaatgccg gatgcggcgt aaacgcctta tccggcctac
    14341 atacgggcga ctcgtaggcc tgataagacg cgacagcgtc gcatcaggca tcgtgcaccg
    14401 aacgccgaag cctcatttat gcctgggcga caactgcttc acaatcatcg ccgcgatccc
    14461 aatcccgtgc tgctgcgcca gggtgttaca caactcatcg tcataaaacc cctgcatcat
    14521 gcgcaccgaa tcgctgttaa acgggttatc tttcgaatca aacaactcgt tggttttacg
    14581 catctctttc agcatgttgc gtaaaaaaat cgcctcaaac tgctccgcgg cctgcttaat
    14641 atcattggcc tgaaccttcg gccccattaa cgcatcgctg ccgtcaatcc cgccgctgcc
    14701 gttgattttc atcagatcac ctccaggtcg gcatccagcg cgcccgcctc atgcagcgcc
    14761 tgtaaaatcg acataatgtc gtccggcgtt gcgcctaaac tgttcagcgc attgaccagc
    14821 gttttcaggc tgctcgactc cggcaaactc accacgccag ggcgcgcatg attcaccgcg
    14881 atattgctct gcggcgttac cgccgtgcgt ccgcctgcaa aggcatttgg ctggctgacg
    14941 ttgctggttt cgttaatcga gacggtcaaa ctgccgtgcg ataccgccgc cgcgtgcagc
    15001 gtaacgccat cacccatcac taccgtgccg gtgcgcgagt tgaacaccac gcgggcgcgg
    15061 attttctccg cctgcacctg cacatcatcc agttgcgaca taaacgccac ccgcgcgccg
    15121 ggactggtag gcgcacgcac ggtaacgttg gtggaactct gcgcggtggc aatgccgcca
    15181 aacgaggcgt tgatcgccgc cgcaatgttg ttggcgtctt taaacgatgg gcgcttcagg
    15241 ttgagggtga tggtgtcgcc catctgaaaa tcgctgggga tctcgcgctc taccgttgcg
    15301 ccgttaggaa ttaacccggc ggttggcgta ttaaccgtta cgctcgaacc gctggccccg
    15361 gtggcattca tcccgcccac caccacgctg ccctgcgcca gcgcataaac ttcgccatcc
    15421 gcgccgtgca actgggtcag caacaaagtg ccgccacgaa tacttttggc atcgccaatc
    15481 gacgaaacag tgacatcaat ggtctgcccg cgcgagtaca tcggcggcaa tgtggcgctc
    15541 accgccaccg cggcgacgtt tttcaccttc gggtcgattt tattcggcaa ctgcacgcca
    15601 aactggcgca gcatattggt gatggtctgg ttggtgaatt tgacctggtt tttatcaccg
    15661 gtaccgtcga ggccgaccac caggctgtag ccgaccagtt ggttttcacg cacaccctgt
    15721 acgttgacca gcgactccag cgattgcgcc agcgccacgc caggcagcgc caagctcacg
    15781 gcgataactg cggcctttat ccagttttgc attgcactct ccggcattaa atcgggaaca
    15841 gaggatggtt aaacaaccgc gtcagccagc ctgcggcgtt ggcgtcgctt aacgcgccgc
    15901 gtccggcata ggagatgcgg gcatcggcga tccgctggga ggaaaccgag ttgtcgttct
    15961 ggatgtcgtc ggcgcgcaca aggccgctta aacggatgta ctcatcgccc tggttgaggg
    16021 tcagccattt ttcgccgcga atttccagga tgccgttggg ctgcaccgca tggacggaaa
    16081 cggtgatctc cccgcgcagg ctgttctgct gctgggaagt ggcgctgccg tcaaagtcgc
    16141 ggttggcgtc gatagagccg gaaagcttat ctttggtgtg tccaaaaatc gttggcgcgc
    16201 cgatatcgac ggtgttgctt ttgccgaagt tggttttcgc ctgtttgctc gactgggtgg
    16261 attcttcgag gatcaccgtc aggatatcgc ccacgcggta agcccgccgg tcggcggtta
    16321 gcgaccagtt gtagccggtt tcaaacacgc ccccggcacg cccgtctgcc ggaggcggcg
    16381 cttcggtggt tggtggcgca aagtatgcgt cgtctttttt gaccagaatg gcctgcgatt
    16441 cacacccgct aaggagtgga agcagcgcca ccagccagat ataatttttc acgttctgcc
    16501 tgtttaaata cagtgagtta tgtgctcttg taggtcggat agtcgcatcc gacatttcgg
    16561 ccttacagcg tctggctgat gtactgcaac atatcgtccg ctgctgacac cattttggcg
    16621 ttcatctcgt acgcgcgttg caccgtaatc atcgccacca tttcgttgac gatatccacg
    16681 ttcgagcctt ccagcgcgtt atcctgcaag gtgccaagcc catcttcgcc cggtacgcct
    16741 tcggtcggct gtccgctggc ggcggtttcc agatagaggt tgtcgccctc ggcagaaagc
    16801 ccggccgggt tggcgaagtt caccagcgtc agttgcccta actcagtcac gtcgctgtcg
    16861 ccgggtaaga tcgccgtcac cgtgccatcc tcaccaaacg cgacgttggt cgcccctgcc
    16921 ggaacgtcga tttccggctg caacggcaag ccctgcgagt tggtcagtac gccgtcagcg
    16981 ttcacctgca agttgccgtc gcgggtataa gcgatatcgc cgttggcctt ttgcacctgc
    17041 aaaaatccct gccccataat ggcgacattc atcgcgttgt cggtggtttc gacgttgcct
    17101 tcggtgaagg ttttctgagt gccgacaatg cgtacgccgc taccgaattg cagcccggtc
    17161 ggcataatgt tgttctgatc gagcatcgcg cccggcgctt cctgggtctg gtaaaacaga
    17221 tcctgaaaca tcacccggtc gcgcttaaag cctgtggtgt taacgttggc gatgttgttg
    17281 gcaatcgcgc tcatttcagc atcctgcgct gagaggccgg ttttgctgat ccatagtgct
    17341 gcgttcatgg tggtaaattc cgtgttattt tttggccgcg gttgccggat gcggcgtgaa
    17401 cgccttatcc gacctacgat gtgcggttac gagccgcgca ataagcggtt gcccgcgtcg
    17461 ctgatatctt cggcggtttt catcatcttg atctgcgctt cgaactggcg gttcatggcg
    17521 atggacgaca tcatctcact caccgccgag acgttgctgc cctcgagaaa accgccgctg
    17581 actttgatgt tttcatcgcg ctgggccgga acgccgtcgg cagtcaccag catcccttca
    17641 ggatttttcg ccaggttcgc gaccgggata tccaccagct tcaggcggtc gatatccatc
    17701 gtcgcggtca cgtcaccgtc atcgggcgtc acggagagcg tgccgtcgct gccaaacgaa
    17761 gcaatggcgt tgggcggcag aatgatcggc ccgttatctc ccagcaccag attgccgtcg
    17821 atagtcagtt gcccctggtc atcctgctga atgttgccgt tgcgggtata cacttcgttg
    17881 ccgtttttat cctgcaccgc aatgtaaccc gcgccctgaa tcgccacgtc cagtgggcgt
    17941 tcggttttct ccgcaacacc agtactgtcg ttcacgccgc tgaccccttc ctgcgccata
    18001 tagcgcgtgt cgaagccgcc gcctttcacc ttgtcattgg tcgccattgc catatcggcg
    18061 cgaaaaccgt tggtgttgac gtttgccagg ttgttggcgc tgatctgctg ctcgtacagg
    18121 gtctgagaag ccccgctcag ggcggtataa atcaagcggt ccattacata gcctggaaca
    18181 gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga tagttacgct
    18241 gggcggtcat caggttgact aactcgctgg tgatatcgac gttagagctt tcgagcacgc
    18301 ccgacgacag cgtgccgcag gtgccggaac cgggaacgcc aatcaacggg gtgccggatt
    18361 cgccggtttg tacccacgcg gtgccgctca ctgcctccag gccgttttca ttcgggaagg
    18421 tcgccagcac cacctgcccc tgcaacatgc gctcgccatt gctgtaggta gcgtaaactt
    18481 tgccatcgtc atcaacctgc acaccgttct gagtggcaga ggcgtagccg ttggcggcgt
    18541 tggtggtgac agaaaattca gagccatatt gcgtacagga ggagtaatcg acggttaaat
    18601 cgatgggtgc ggcggcgtcg gtctgaaact caatggtctg cggcgtgttt ggcgaggtca
    18661 gtttcccggt gttcgggtcg aaggttaacg tcgtcgccgg aacgccggtc tgctgctgac
    18721 cgtcgaaggt gtactgcact tcccaggtgt tatcgctggt tttggtgaag tactggcata
    18781 ccgaatgttc gttgcctaat gagtcgtata ccgtggtggt gtagctgtcg gtgtacgagc
    18841 tgctgttggt cgcgtcgaac ggtacggttg tgcggtcgat agcgtcgtcg ctggcatcga
    18901 agttggcggt aaaggtcagg ctgctgctgg cctgcgccgg aatattaccg gttttgatct
    18961 ggatatcggt gactgtaccg gtttgcaggg tgcccgtgtc atcgaccgga tagccctgca
    19021 aataagcacc cgaggcgttg acgatatagc cgtttttgtc ggtttcaaac gatccggcgc
    19081 gggtataaga aatgttgccc gcgctgtcgc aggtaacaaa aaagccgtcg tcgttaatcg
    19141 ccagatccag cgcgttaccg gtggatacca gcgaaccgcc gcgcgaaata ctttgcgcgg
    19201 tgcctgccac gctgacgccc atcgcctgac ttccggcata catggctgaa aactgggtgg
    19261 tcatcgactt atagcctacc gttccggcgt tggcgatgtt gttactgatc ccgtccagct
    19321 gttcgttaac ggcattcagc cccgttgcag caatttcata actcattgtt tcgtcctgtt
    19381 aattcaaatt tttggcagcg tgcccgttgt tttgccggat tcggcgtgaa cgccttatcc
    19441 ggcctacgaa cgggtgcaaa cataaaaata tgcgcgaact cgtaggcctg ataagacgcg
    19501 ttaagcttcg catcaggcat cttgcaccga acgccggatg cggcgtgaac gccttatccg
    19561 gcctacaaaa ccatgcagtt tcatgtaggc ctgataagcg aagcgcatca ggcaatttgg
    19621 caccggacat cacgccacat cactgtcggc atcgggtacg ccaaactggc taatcatggt
    19681 gaacggtact tcgcccacgc ctgcaacgtt aagcaccggg gagctgccgt cgagtgggat
    19741 gcgcacatct tccacttcgc cggagacttc aatcggcacc tcttcttccc cggtgttggt
    19801 caccaccgag acgtcgtaat cgcccggcgg gatgccgtaa tccgccggat taattgagaa
    19861 atcgacctgg ccgggtccaa cggacgaact gccttccggg atcagcgaca ccgtatagtc
    19921 atcgcctgcg gcatcggtaa agtggagatc ggcggtggta caggcgtcgt ccagcgtggc
    19981 gcgaccgtta atggtctggt cactcacctg caaggcggtg gtctgcacca taatgtcgtc
    20041 gcccaccagg ttgccgagcg ccatcacctg aatgttgctc atcagcaccg cgttggtatt
    20101 cgcctgcacg ctcatctctt ccatcatcgc cacctgcgcc attgacgaaa gctggtcgat
    20161 atattcggtg gcgtcggtcg ggtcggtcgg gtcctggttt tggatctcag cgacaaacag
    20221 cgtcaggaaa gtatcaacgg gcgagctatc gctgctgctt gtcgtgtcgc tggccgccac
    20281 gttgttgctt tccgccgtgg cggtcggctg cggtgattgc gcattcagcg ccagggtatt
    20341 catcaggctt ctcccagttt cagcaaactt tgctgcatac ttttcacgtt gttgagcaca
    20401 tcgacattgg tttcgaaatc gcgcgaagcc gacatcatgt ccgccatctg ttccaccacg
    20461 ttgacgtcgg gataccagac atccccgtta gcatcggcga gcggcgaatg tggttcatag
    20521 cgtttcacag ccccgccggt ttgcagcacg tcctgcacct gcacactggc accgtcaatg
    20581 gcatgacgat gtgtacccgc cagcaaactg tggtgataca ccgccgcaaa caccgggctg
    20641 cgcgctttat atgcctgcgc ctcgctgctg gcgggcgcat tggcattcgc caggttactg
    20701 gcaacggtat tcagacgtag cgtttgcgcc gtcatcgccg agccggaaat ctgatagata
    20761 tcagtaaatg acatgcttat ttcccttcaa tggcctctct gatgccgctg atttgcagat
    20821 tcagaaaggt cagactgctt tgataatcca tactattttg tgaaaaccgc gcctgttcgc
    20881 tgttcagttc cacggtgttg ccatcttccg acggctgcat cggcacacgg tactggacat
    20941 ccaccgccgc gttatttgcc cgttgcatct ccctggcaaa atcaatatct ttagccttga
    21001 aatttggcgt atcgacgttc gccagattcg cagtcagtag ctcggtcctc tcaagacgta
    21061 acttcactgc ctgcggatgt actcccaatg cctgctgaaa actgatcccc atttcctgcg
    21121 tcactcctgc ttcaatccgc atacgctaag caatcgccgt gccatttttt aagtgactga
    21181 agagaaaggg tttatgagtt ttcaattttg caggcggaag ttttatttcc tcctggcgct
    21241 ggccttaccg ggctacgccg ccgtccatcc cgtgcagcac agcgcgcggg aacaagtcaa
    21301 cgcccaggta ctgaatgccg ccagccaaaa aattgaatcg ctggcgcaac agcggcgctg
    21361 gcatgactat cgctacacct ttaaggtcta cattccctcg cagattgcga ccgccgcgcc
    21421 ctgtactaaa acacccggcg tcacgctgac ttcgcctggg gacatcgccc tcaaccggat
    21481 gaacttcacc gtcagttgcc cacaaagctg gcagatgaac gtagcggtgc gcccggatgt
    21541 actggtgcca gtagtaatgg cgaagtcact ggttgcccgc gatacgccgc tcaccgcgaa
    21601 tgatgtggaa ctgagaccct acaacgtcag tgcccagcgc cgggaggtgt tgatggaacc
    21661 caatgacgcg attggtttca gcagtaaaca cgcgctgcaa ccgggcaggc caatcaccaa
    21721 agaagaactg atatctccgg tgctggtcgg gcgcgatcaa ccggtgatga ttgtttacca
    21781 gagcgcgggg atcaccgcct caatgcccgg cgtagcgctg aaaaatggtc gcaaagggga
    21841 aatggtgaag gtgcgtaaca ccagcagcca gcgggtcatt agcgcgatgg tggcggaaag
    21901 cggcgtggtg acaacggtaa gcgcggagta atttgcgctg gcgattaaag ttttgccgcc
    21961 tgttaaccgc tttaccggag taacccaccc aaataccgag acgaggttgc gatgaaaatc
    22021 accccgacta tgccaggcaa tcacccgaca gccacaatgg caggctccag tcagccgcgc
    22081 aaaagcacgg cgaccaccgc cacgacgctg agcgctgacg acattaccca ggccggattg
    22141 cagtctgctc agcaaacgct gaacgaccag caagagagcg atatcgacag cgataaagtg
    22201 gcacaaatgc aggcgatgct agcctccggc agcctgcaag tggacaccga acagttagcc
    22261 agcgacatgc tgagtttttt ccagaactaa gaggccgatg aagtgagtat ccggttacaa
    22321 caggtgaagg cgttgttgca gggaattcgt gacgacgacg cactgtacga ttccctgcgt
    22381 gaacttctcc agcgtcagcg gatctgcatg attcgccgcg ccagcgagga tctgctggcg
    22441 gtaaacgacg agataacgca gcactatgag cagttgcatg ggcatagcta tcagcggcat
    22501 agcctgctga aaatgctcgg tgttagcgta aatcgtgacg gtctggcgca ggtgtttgcc
    22561 tggcttccgg cggtacaaaa agcggcagca caacagttgt ggcaacgcct tgagcaaaaa
    22621 gcagaacgct gcaaagccta taacgataaa aacggcgagc tgttaattcg ccagtatgaa
    22681 tttattcagg cttttttagg cagcgaagcg gattttctct atcagggata acgacaattc
    22741 cttcccccat cgcggaaacg gattttccgc ctgattctca atcccttgtt tttgattaca
    22801 aaaattcccg gcacgcttat tgcatattcc gcaaagtaat aaagactgga agccacctat
    22861 gagttttatt gaatgttacg aaccggaata cgtgcgcaat tttatgacgc aatatcccga
    22921 ttcgcatttg tacgtccgca aggtgtggaa aaacgagatt cgccagagcc tggcattgac
    22981 cgatattgaa agttgcaaag cggtgctcaa cgatgccagg gcctttgatt tacagctcac
    23041 ctaccgccct gtggaagaca atgccgccga gctatccgcg cgcgatgcga tagtaaacca
    23101 ggccattttg agcaccctta cgctccccga tttaaccccg gagctttccc tgtatgccgt
    23161 cggaattgtg ctctcccgcg ccagtaaaat gccgggcaga gacggagata ttcttgcccg
    23221 cttgacgacg ctgccacagg cgctggccga tcattcacaa aaaggcacac tacaggcaca
    23281 gttcgcccag ctgccgcccg tgccacagct ggcgcgccag ttgatgacgc tgctcgggag
    23341 ttttacgttt gactggtcga ttctaccgga aagcccacgc aaggcctcgc tgccgttgca
    23401 ggttaccttg ctgacgctac atgacgccaa cagcaaagcc ctgttacaac agcagcttaa
    23461 ggtgcagtgg caaaccacct ggcaacagca ttttgccacc gcgccgtgga tgatgcgtaa
    23521 ctggctgatt taccgcgttt accatgaggt gattggacag acagacggcg cagattattg
    23581 cccgctggtg tgcgattttt atcttatccg cacgttaatc agtttatgga cgctggatgg
    23641 ttctccgctg cgacaggagg atattttcgc cctcttcgcc atgtttgaac gctggcgagc
    23701 cagtgaaaat gccgtgctga ttcgccaaca aattcaggct gaatgttccg cagatcccct
    23761 gctttatgcg ttttcactgc tgtcttgcta acgctttctg gccggttttc ccggccttat
    23821 cgacttcgta aactatcatg caaaccctca tcaaaacact cgccaccggg cgcgtaaaac
    23881 cgtttgtcag cgtcgtcacg ccaacctgga accgcggcac attcctgccg tacctgcttt
    23941 atatgtaccg ctatcaggat tacccggcag accgccgcga actcatcatc ctcgacgact
    24001 ccccgcaaag ccatcaacac atcatcgacc gcctgaccaa tggcacgccg ggatccttca
    24061 acatccgcta catccatcac ccggaaaaac tgccgctggg caaaaaacgc aatatgctca
    24121 acgaactggc gcgcggcgaa tacatcctct gcatggacga tgacgactac tactcgccag
    24181 ataaaatctc ttactccatc gccatgatgc aaaaacaccg ggcgctcatc tccggttccg
    24241 accagatccc catctggtac agccacatca accgcatttt caaaagccgc agctttggcc
    24301 cacataacat cctcaatggc accttttgct atcaccgtaa ctacctgaaa aagcaccgtt
    24361 acgacgacga ttgtaattta ggcgaggaga aaagtttcac caacaatttc agcgttaacc
    24421 cgctgcaact gcccggcgag cgcacgattc tgtgtatctc gcacagccac aacacctttg
    24481 ataaagactt tatcctcggg gccagcacgc ctgtaaacgc ggcgcttacg gatatcgtca
    24541 gcgatccgct gctacgcaac gcttatctgg gtctgcataa cgccactcat tatcaggcga
    24601 tccagcatca ggcgatcgat cagatcgttc tgctgaatct ggataaacgc ccggatcgct
    24661 tacagcagat ccgtgaagaa ctgacattgc tgcatattcc gcctgagaag atcacccgcc
    24721 ttgccgccag tgaaaatgaa aacggtcaac ggggccgcca gcaatcacac ttgcaggcgc
    24781 tgcatctggc gcaacagcac ggctggcaaa actatctgct gctggaagat gacgcggtta
    24841 ttctcaaaca ggagaaacat attcaggtgc tgaacgcgct gctggcgagc ctggcgaaaa
    24901 ttccctggca ggtgatgatc ctcggcggcg aaatttcgca gggaacgatg ctaaaaagtt
    24961 tgcctgggct ggtgcacgcg cgggactgcc gcaaagtgtg cgcttatctg gtgaacagcc
    25021 gctactaccc gcagctggcg cagcagatga acaacgatga gcattcgctg gaagacggct
    25081 ggcagccgct gctgcgcacc gacaaatggc tggcctgtta cccgagcctt tgttatcagc
    25141 gaccgggctt tagtgatatc gagaaaaaag cgactgataa cattaaccac tacttcaata
    25201 agttacccgt agccaccaag ccatctacat tacctatcgc cgataccatc ggctttttta
    25261 tggaaacctc attccactac gcgctctacc gcccaatcat cactgcccta caggcgcaag
    25321 gacaaagctg cacgctggtg atcaacgacc gcgtgtttaa accgtttctg gatgaaatgc
    25381 tgaatacgtt aaaaagcatc gacgatccac agctcaaagg tatgcgctta tctgaaatgc
    25441 aggcacacgg tcagcgagta aaatgcctgg tcagccccta ttacacgcct gcgctcagtg
    25501 gcctttcccc cctgaacatt cgcgccatgt acggcttagc caaagaaacg tggaaccacg
    25561 ccgactggaa ccgtttttat cagcgcattt tgtgttacag ccactacagt cagcgggcgc
    25621 tggcacattt cggcagcgca aaagtggtcg gcaatccgcg cttcgacgcc tggcataacg
    25681 gcacgtttga ccgcgctctg ccggaaaaca tccaacccga ttcccgtaaa cctacggtgc
    25741 tctacgcccc cacgtttggc gcattaagct ccctgcccca ctgggcagaa aaattgggcc
    25801 gcttaagcgg cgatgtgaac ctgatgtgca aactgcacca cggcacctgc tcgcgcccgg
    25861 aagaagccgc atcgctggca ctggcgcgca ggcatttaaa acagcgcacc gactctgccc
    25921 gccacacgct ggcgctactg gcaaaggccg attacgtgct gaccgataac agcggcttta
    25981 ttttcgatgc tatccacgtt gataaacggg tgatcctgct cgactttccg ggcatgacaa
    26041 cgttgctcga cggtgagaaa agttattcca cgcccgaaag tgccgaccag caaattcgcg
    26101 aaatattgcc ggtggctcat gatgttgcgg aactgcgcta cttattgtca gaggcgtttg
    26161 actggggtgc ggtgcatgcg aggcttactg agattcgcca tcactattgc gatgcgttta
    26221 tggatggcaa agcgggagaa cgagcggcgt tggtgattgt ggaggcacta gggtaaaacg
    26281 cgcaagtgcc tgatgcgctt cgcttatcag gcctacgcga ccttgcaata gtttaaattt
    26341 gtgaggattt gcaggtcgga taaggcgctt acgccgcatc cggcacgaac aacgcactta
    26401 actaccctct gatttcaaac tcatcgcttt atgactggtg aatcggtaaa agtcgtagat
    26461 tgtgggggag cgatctttat gaattctgaa aaaacctgaa tcttgctaaa catattgctt
    26521 atcataaata taggttacac actataacaa ttattgcgta atttaacatt ttgttaaaat
    26581 tgatagagca taaacgataa catgacttat gataaaaaag taacttccgg tacaacaagc
    26641 cagaaacaaa acaacattgt cactcaaaaa atatctcgcc caaaggaatt atttctatta
    26701 ttttgggttg atgagtccgg tgata
//
LOCUS       DAZFRG010000040        26686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFRG010000040 DAZFRG010000000
VERSION     DAZFRG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.040"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..776
                     /gene="ycaL"
                     /locus_tag="ACHKUC_001343"
     CDS             12..776
                     /gene="ycaL"
                     /locus_tag="ACHKUC_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO6617628.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            949..1632
                     /gene="cmk"
                     /locus_tag="ACHKUC_001344"
     CDS             949..1632
                     /gene="cmk"
                     /locus_tag="ACHKUC_001344"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO6617629.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1743..3416
                     /gene="rpsA"
                     /locus_tag="ACHKUC_001345"
     CDS             1743..3416
                     /gene="rpsA"
                     /locus_tag="ACHKUC_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO6617630.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDIVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3576..3860
                     /gene="ihfB"
                     /locus_tag="ACHKUC_001346"
     CDS             3576..3860
                     /gene="ihfB"
                     /locus_tag="ACHKUC_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO6617631.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4066..6330
                     /gene="ycaI"
                     /locus_tag="ACHKUC_001347"
     CDS             4066..6330
                     /gene="ycaI"
                     /locus_tag="ACHKUC_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO6617632.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIVLTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVSKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVIWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6367..8115
                     /gene="msbA"
                     /locus_tag="ACHKUC_001348"
     CDS             6367..8115
                     /gene="msbA"
                     /locus_tag="ACHKUC_001348"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO6617633.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8112..9098
                     /gene="lpxK"
                     /locus_tag="ACHKUC_001349"
     CDS             8112..9098
                     /gene="lpxK"
                     /locus_tag="ACHKUC_001349"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO6617634.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGH"
     gene            9135..10367
                     /gene="ycaQ"
                     /locus_tag="ACHKUC_001350"
     CDS             9135..10367
                     /gene="ycaQ"
                     /locus_tag="ACHKUC_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO6617635.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGDYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYIPAPKRQYGYYVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            10419..10601
                     /gene="ycaR"
                     /locus_tag="ACHKUC_001351"
     CDS             10419..10601
                     /gene="ycaR"
                     /locus_tag="ACHKUC_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO6617636.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQEIICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10598..11344
                     /gene="kdsB"
                     /locus_tag="ACHKUC_001352"
     CDS             10598..11344
                     /gene="kdsB"
                     /locus_tag="ACHKUC_001352"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6617637.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11498..12391
                     /gene="ycbJ"
                     /locus_tag="ACHKUC_001353"
     CDS             11498..12391
                     /gene="ycbJ"
                     /locus_tag="ACHKUC_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO6617638.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12368..13147)
                     /gene="elyC"
                     /locus_tag="ACHKUC_001354"
     CDS             complement(12368..13147)
                     /gene="elyC"
                     /locus_tag="ACHKUC_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO6617639.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13283..14068
                     /gene="cmoM"
                     /locus_tag="ACHKUC_001355"
     CDS             13283..14068
                     /gene="cmoM"
                     /locus_tag="ACHKUC_001355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO6617640.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14065..15387
                     /gene="mukF"
                     /locus_tag="ACHKUC_001356"
     CDS             14065..15387
                     /gene="mukF"
                     /locus_tag="ACHKUC_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO6617641.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15368..16072
                     /gene="mukE"
                     /locus_tag="ACHKUC_001357"
     CDS             15368..16072
                     /gene="mukE"
                     /locus_tag="ACHKUC_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO6617642.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16072..20532
                     /gene="mukB"
                     /locus_tag="ACHKUC_001358"
     CDS             16072..20532
                     /gene="mukB"
                     /locus_tag="ACHKUC_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO6617643.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVAALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20793..22640
                     /gene="ldtD"
                     /locus_tag="ACHKUC_001359"
     CDS             20793..22640
                     /gene="ldtD"
                     /locus_tag="ACHKUC_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6617644.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22821..23369
                     /gene="mepK"
                     /locus_tag="ACHKUC_001360"
     CDS             22821..23369
                     /gene="mepK"
                     /locus_tag="ACHKUC_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO6617645.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTC
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23396..24043
                     /gene="gloC"
                     /locus_tag="ACHKUC_001361"
     CDS             23396..24043
                     /gene="gloC"
                     /locus_tag="ACHKUC_001361"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO6617646.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24093..25283)
                     /gene="aspC"
                     /locus_tag="ACHKUC_001362"
     CDS             complement(24093..25283)
                     /gene="aspC"
                     /locus_tag="ACHKUC_001362"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO6617647.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25468..26556)
                     /gene="ompF"
                     /locus_tag="ACHKUC_001363"
     CDS             complement(25468..26556)
                     /gene="ompF"
                     /locus_tag="ACHKUC_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO6617648.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6472 a         6531 c         7243 g         6440 t
ORIGIN      
        1 ggatgattca catgaagaat actaaattat tgctggcgat tgcgacctct gcagccttac
       61 tgacaggatg tcagaatacg cacggtatta ataccgattt ggctatcagc tcaggtctga
      121 atgcttataa agccgcaaca ttaagcgatg ctgatgctaa agcaatcgcc aatcagggct
      181 gtgtcgaaat ggacagcggc aataaagtgg caagcaaatc cagcaagtac gggaaacgtc
      241 tggcaaaaat cgccaaagca ttgggtaaca atattaatgg cacgccagtc aactataagg
      301 tttatatgac cagcgacgtc aacgcatggg cgatggcgaa cggttgtgtt cgtgtctaca
      361 gtggcctgat ggacatgatg aacgacaacg aaattgaagg cgttctgggc catgaactgg
      421 gccacgtcgc gttgggtcac tcgctggctg aaatgaaagc ttcttatgcg atcgtggccg
      481 cacgcgatgc catttcagct accagcggtg tggcttccca gctttcccgt tcacaattag
      541 gtgatatcgc agaaggcgct atcaatgcta aatactcccg tgataaagaa tccgaagcag
      601 atgatttctc ttttgatctg ttgaagaaac gtggcatcag tacccagggg ctggttggta
      661 gctttgaaaa actggccagc ctggatggcg gtcgcaccca gtccatgttt gattctcacc
      721 caccatcaac agagcgtgcg caacacatcc gtgatcgtat cgcctctggt aagtaaatca
      781 ttgtcatctt tcgggctggt cttctgcctg cccgctataa ttgcgcaata aatccccatc
      841 tgaatacaga caaaactggt ttttgcacac aacgttaacg atttgtggcg tcggcgcgta
      901 taatgcgcgc ggttatgtta acggtacgcc tgttttaagg agataaagat gacggcaatt
      961 gccccggtta ttaccattga tggcccaagc ggtgcaggga aaggcacctt gtgtaaggct
     1021 atggcggaag cgttgcaatg gcatctgctg gattcgggtg caatttatcg cgtactggca
     1081 ctagcggcat tacatcacca tgttgatgtt gcatcggaag atgcgctggt gccgctggca
     1141 tcccatctgg atgtacgttt tgtgtcaacc aacggcaatc tggaagtgat cctcgaaggg
     1201 gaagatgtca gcggcgaaat tcgtactcag gaggtggcga atgctgcttc acaagtcgcg
     1261 gcattcccac gcgttcgtga agcattattg cgtcgccaac gcgcgtttcg cgaattacca
     1321 ggcctgattg ccgatgggcg cgacatggga acggtggtat tccctgatgc gccagtgaaa
     1381 attttccttg acgcctcctc ggaagaacgt gcgcatcgcc gcatgctaca gttgcaggag
     1441 aagggcttta gtgttaactt tgagcgcctt ttggccgaga tcaaagaacg tgacgaccgc
     1501 gatcgtaatc gagcggtagc gccactggtt ccggcagccg atgctttagt gttggattcc
     1561 accaccttaa gcattgagca agtgattgaa aaagcgctac aatacgcgcg ccagaaattg
     1621 gctctcgcat aagcgaccga atttgcagta cccccgttgc aatggaatga cagcgggtat
     1681 gttaaacaac cccatccggc atggagccag gtggacgtta aatataaacc tgaagattaa
     1741 acatgactga atcttttgct caactctttg aagagtcctt aaaagaaatc gaaacccgcc
     1801 cgggttctat cgttcgtggc gttgttgttg ctatcgacaa agacgtagta ctggttgacg
     1861 ctggtctgaa atctgagtcc gccatcccgg ctgagcagtt caaaaacgcc cagggcgagc
     1921 tggaaatcca ggtaggtgac gaagttgacg ttgctctgga cgcagtagaa gacggcttcg
     1981 gtgaaactct gctgtcccgt gagaaagcta aacgtcacga agcctggatc acgctggaaa
     2041 aagcttacga agatgctgaa actgttaccg gtgttatcaa cggcaaagtt aagggcggct
     2101 tcactgttga gctgaacggt attcgtgcgt tcctgccagg ttctctggta gacgttcgtc
     2161 cggtgcgtga cactctgcac ctggaaggca aagagcttga attcaaagta atcaagctgg
     2221 atcagaagcg caacaacgtt gttgtttctc gtcgtgccgt tatcgaatcc gaaaacagcg
     2281 cagagcgcga tcagctgctg gaaaacctgc aggaaggcat ggaagttaaa ggtatcgtta
     2341 agaacctcac tgactacggt gcattcgttg atctgggcgg cgttgacggc ctgctgcaca
     2401 tcactgacat ggcctggaaa cgcgttaagc atccgagcga aatcgtcaac gtgggcgacg
     2461 aaatcactgt taaagtgctg aagttcgacc gcgaacgtac ccgtgtatcc ctgggcctga
     2521 aacagctggg cgaagatccg tgggtagcta tcgctaaacg ttatccggaa ggtaccaaac
     2581 tgaccggtcg cgtgaccaac ctgaccgact acggctgctt cgttgaaatc gaagaaggcg
     2641 ttgaaggcct ggtacacgtt tccgaaatgg attggaccaa caaaaacatc cacccgtcca
     2701 aagttgttaa cgttggcgat atagtggaag ttatggttct ggatatcgac gaagaacgtc
     2761 gtcgtatctc cctgggtctg aaacagtgca aagctaaccc gtggcagcag ttcgcggaaa
     2821 cccacaacaa gggcgaccgt gttgaaggta aaatcaagtc tatcactgac ttcggtatct
     2881 tcatcggcct ggatggcggc atcgacggcc tggttcacct gtctgacatc tcctggaacg
     2941 ttgcaggcga agaagcagtt cgtgaataca aaaaaggcga cgaaatcgct gcagttgttc
     3001 tgcaggttga cgcagaacgt gaacgtatct ccctgggcgt taaacagctc gcagaagatc
     3061 cgttcaacaa ctgggttgct ctgaacaaga aaggcgctat cgtaaccggt aaagtaactg
     3121 cagttgacgc taaaggcgca accgtagaac tggctgacgg cgttgaaggt tacctgcgtg
     3181 cttctgaagc atcccgtgac cgcgttgaag acgctaccct ggttctgagc gttggcgacg
     3241 aagttgaagc taaattcacc ggcgttgatc gtaaaaaccg cgcaatcagc ctgtctgttc
     3301 gtgcgaaaga cgaagctgac gagaaagatg caatcgcaac tgttaacaaa caggaagatg
     3361 caaacttctc caacaacgca atggctgaag ctttcaaagc agctaaaggc gagtaattct
     3421 ctgactcttc gggattttta ttccgaagtt tgttgagttt acttgacaga ttgcaggttt
     3481 cgtcctgtaa tcaagcacta agggcggcta cggccgccct taatcaatgc agcaacagca
     3541 gccgcttaat ttgcctttaa ggaaccggag gaatcatgac caagtcagaa ttgatagaaa
     3601 gacttgccac ccagcaatcg cacattcccg ccaagacggt tgaagatgca gtaaaagaga
     3661 tgctggagca tatggcctcg actcttgcgc agggcgagcg tattgaaatc cgcggtttcg
     3721 gcagtttctc tttgcactac cgcgcaccac gtaccggacg taatccgaag actggcgata
     3781 aagtagaact ggaaggaaaa tacgttcctc actttaaacc aggtaaagaa ctgcgcgatc
     3841 gcgccaatat ttacggttaa gtttttactc aaacttgaac gcgagaaaag cacctgtcgg
     3901 gtgctttttt catttctcta atctggaact ggaagctgcc tcgcacagtt ttgagcagtt
     3961 ttcaccctat cgttaaattc ttctgaacgt gcctcgggga acgcaaaact cccaccagac
     4021 aaccgttcaa caaagttgca cactttctat aaacagggag ggtgcatgaa aataacgaca
     4081 gtcggcgtat gcataatttg cggaattttt ccgttgctga ttttgcccca attgcctggg
     4141 acagtaaccc ttgcgtttct gactctcttc gcctgtgtac tggcatttat ccctgttaaa
     4201 accgtccgtt atatcgtgct gacgttgctg tttttcgttt ggggcatatt agcagcaaag
     4261 caaattttgt gggcaggaga aaccttaact ggcgcgacgc aggatgcaat tgttgagatc
     4321 attgctactg acggcatgac cactcattac ggtcaaatta ctcatctaca aggtcgacgt
     4381 atattccctg cgccaggcct cgtactgtat ggcgaatatc ttccgcaagc ggtttgtgcc
     4441 ggacaagtat ggtcaatgaa actcaaagtt cgtgcagttc atggtcaact taatgatggc
     4501 ggctttgata gccagcgtta tgccattgcc cagcatcaac cgctcaccgg ccgttttctg
     4561 caggcaagtg tcattgaacc gaattgtagc ctgcgtgcac agtatctggc gtcactacaa
     4621 acaacgctgc aaccctatat gtggaacgcg gttattcttg gattaggtat gggggaacgg
     4681 ttatccgtct ccaaagaaat caaaaatatc atgcgtgata ctggaacggc gcatttaatg
     4741 gcgatatcag gattgcacat cgcttttgcg gcgttgctgg ctgcaggact cattcgcggt
     4801 gggcaagttt ttctgcctgg gtgctggatc cactggcaaa tgccattaat tggtggaatc
     4861 tgctgtgctg ctttttatgc ctggcttacg ggaatgcaac ctcctgcatt gcgtaccgtg
     4921 gtggcgcttg ccatttgggg aatgcttaag ttaagtggac gacaatggag tggctgggat
     4981 gtatggatat gttgtctggc ggcaattttg ctgatggatc ctgttgccat tctctcgcaa
     5041 agtttatggc tctctgccgc tgcggtcgcg gcactgattt tttggtatca gtggtttccc
     5101 tgtccggagt ggcagctgcc gccggtattg cgcgcggttg tttccctcat ccatctgcaa
     5161 ctgggaatca ccctcctgct tatgcccgtg caaattgtca tatttcatgg cattagtctg
     5221 acctcgttta ttgcaaatct ttttgcaatt cccctggtga catttatcac ggttccgttg
     5281 attctcgccg cgatggttgt gcatttaagc gggccgttta ttctggaaca agggttatgg
     5341 tttcttgccg accggtcttt ggctttactt ttctgggggt taaagagttt gccggaaggg
     5401 tggatcaaca ttgctgaacg ttggcaatgg ctaacatttt ccccatggtt cttactggtg
     5461 atatggcgat taaacgcctg gcgaacgttg ccagcaatgt gtgtggctgt aggcttgctg
     5521 atgtgcttgc cgctgtggca aaaacctcga cctgacgagt ggcaagtgta catgcttgat
     5581 gtcgggcaag ggctggcaat ggtcattgcg cgaaacggca aagcgattct ctatgatacg
     5641 ggactggcct ggcccgaagg ggatagtggg caacaactga ttatcccctg gctccactgg
     5701 cataatcttg aaccggaagg cgttattttg agtcatgaac atctggatca ccggggaggg
     5761 ctggactcaa tattgcacac atggccgatg ttatggatcc gaagtccgtt aaactgggaa
     5821 catcatcagc cctgtgtgcg tggcgaagcg tggcaatggc aaggattgcg tttcagcgcg
     5881 cactggcctt tacaaggtag caacgataaa ggaaataacc attcctgtgt ggttaagatt
     5941 gatgacggga cgaatagcat tcttctaacc ggtgatattg aagcccccgc tgaacaaaag
     6001 atgctaagcc gttactggca gcaaatgcag gcaacattac ttcaggtacc tcaccatggc
     6061 agtaatacct catcatcgtt gccattaatt cagcgagtga atggaaaagt ggcactcgca
     6121 tcggcatcgc gctataacgc atggcgactg ccctcaagca aagttaaaca tcgctatcaa
     6181 caacagggtt ataagtggct tgatactcca catcagggtc aaataacggt caatttttca
     6241 gcgcaaggct ggcggattag cagcctcaga gagcaaattt tacctcgttg gtatcatcag
     6301 tggtttggcg tgccagtgga taacgggtag aatatgcggc tatttcaaca aatgctggtt
     6361 ttttgaatgc ataacgacaa agatctctct acgtggcaga cgttccgccg actgtggcca
     6421 accattgcgc cttttaaagc gggtctgatc gtggcgggcg tagcgttaat cctcaacgca
     6481 gccagcgata ccttcatgtt atcgctcctt aagccacttc ttgatgatgg ctttggtaaa
     6541 acagatcgct ccgtgctgat gtggatgccg ctggtggtga tcgggctgat gattttacgt
     6601 ggtatcacca gctatatctc cagctactgt atctcctggg tatcaggaaa ggtggtaatg
     6661 actatgcgtc gccgcctgtt tggtcacatg atgggaatgc cggtttcatt ctttgacaaa
     6721 cagtcaacgg gtacgctgtt gtcacgtatc acctacgatt ccgaacaggt cgcttcttct
     6781 tcttccggcg cactgattac tgttgtgcgt gaaggtgcgt cgatcatcgg cctgttcatc
     6841 atgatgttct attacagttg gcaactgtcg atcattttga ttgtgctggc accgattgtt
     6901 tcgattgcga ttcgcgttgt atcgaagcgt tttcgcaaca tcagtaaaaa catgcagaac
     6961 accatggggc aggtgaccac cagcgccgaa caaatgctga aaggccataa agaagtattg
     7021 attttcggtg gtcaggaagt ggaaacgaaa cgcttcgata aagtcagcaa ccgaatgcgt
     7081 cttcagggga tgaaaatggt ttcagcctct tctatctctg atccgatcat tcagctgatc
     7141 gcctctttgg cgctggcgtt tgttctgtat gcggcaagct tcccaagtgt catggatagc
     7201 ctgactgccg gtacgattac cgttgttttc tcttcaatga ttgcacttat gcgtccgctg
     7261 aaatcgctga ccaacgttaa cgcccagttc cagcgcggta tggcggcttg tcagacgctg
     7321 tttaccattc tggacagtga gcaggagaaa gacgaaggta agcgcgtgat cgagcgtgcg
     7381 actggcgacg tggagtttcg caatgtcacc tttacttatc cgggacgtga tgtgcctgca
     7441 ttgcgtaaca tcaacctgaa aattccggca gggaagacgg ttgctctggt tggacgatct
     7501 ggttcaggta aatcaaccat cgccagccta atcacacgtt tttacgatat tgatgaaggc
     7561 gaaatcctga tggatggtca cgatctgcgc gagtataccc tggcgtcgtt acgtaaccag
     7621 gttgctctgg tgtcgcagaa tgtccatctg tttaacgata cggttgccaa caacattgct
     7681 tacgcaagga ctgaacagta cagccgtgag caaattgaag aagctgcgcg tatggcctac
     7741 gccatggact tcatcaataa gatggataac ggtctcgata cagtcattgg tgaaaacggc
     7801 gtgttgctct ctggcggtca gcgtcagcgt attgctatcg ctcgagcctt gttgcgtgat
     7861 agcccgatac tgattctgga cgaagctacc tcggcgttgg ataccgaatc cgaacgtgcg
     7921 attcaggcgg cactggatga gttgcagaaa aaccgtacct ctctggtgat tgcccaccgc
     7981 ttgtctacca ttgaaaaggc agacgaaatc gtggttgtcg aggatggtgt cattgtggaa
     8041 cgcggtacgc ataacgattt gcttgagcac cgtggcgttt acgcgcaact tcacaaaatg
     8101 cagtttggcc aatgatcgaa aaaatctggt ctggtgaatc ccctttatgg cggctattgc
     8161 tgccactctc ctggttgtat ggccttgtga gtggcgcgat ccgtctttgc tataaactaa
     8221 aactgaagcg cgcctggcgt gcccccgtac cggttgtcgt ggttggtaat ctcaccgcag
     8281 gcggcaacgg aaaaaccccg gtcgttgtct ggctggtgga acagctgcaa cagcgcggta
     8341 ttcgcgtggg ggttgtatcg cggggatatg gtggtaaggc tgaatcttat ccgctgttat
     8401 tgtcggcaga taccaccaca gcacaggcgg gtgatgaacc tgtgttgatt tatcaacgca
     8461 ctggcgcgcc tgttgcggtt tctcccgtgc gttctgacgc ggtaaaagcc attctggcgc
     8521 aacaccctga tgtgcagatc atcgtaaccg acgatggttt acagcattat cgtctggcgc
     8581 gtgatgtaga aattgtcgtt attgatggtg tgcgtcgctt tggcaatggc tggtggttgc
     8641 cggcggggcc aatgcgtgag cgagcggggc gcttaaagtc ggttgatgcg gtaatcgtca
     8701 acggcggtgt cccccgcagc ggtgaaatcc ccatgcatct gctgccgggt caggcggtga
     8761 atttacgtac cggtacgcgt tgtgacgttg ctcagcttga acatgtggtg gcgatggcag
     8821 ggattgggca tccgccgcgc ttttttgcca cgctgaagat gtgcggcgta caaccggaaa
     8881 aatgtgtacc gctggccgat catcagtctt tgaatcatgc ggatgtcagc gcgttggtaa
     8941 gcgccggaca aacgctggta atgactgaaa aagatgcggt gaaatgccgg gcctttgcag
     9001 aagaaaattg gtggtatttg cctgtagacg cacagctttc aggtgatgaa ccagcgaaac
     9061 tgcttgcgca actaacctcg ctggcttctg gccactagtt acgccgcggc agcgttcgat
     9121 tgatggagtc atgaatgtcg ctgccgcacc tctcccttgc tgatgcgcgt aatcttcacc
     9181 ttgccgcgca aggcctgtta aacaaacccc gccgtcgagc gtcgttggag gatattccgg
     9241 caacgatctc ccgcatgtcc ttgctgcaaa tcgataccat caatattgtt gcccgtagcc
     9301 catatctggt gcttttcagc cgtctgggag attatcctgc gcagtggctg gatgagtctc
     9361 tggcgcgtgg cgaattaatg gaatactggg cgcatgaagc ctgctttatg ccgcgtagcg
     9421 attttcgtct tattcgccac cgcatgctgg cacctgaaaa aatgggctgg aaatataaag
     9481 acgcctggat gcaggaacat gcggcggaaa ttgcactgtt aattcagcat attcatgata
     9541 aggggccggt acgttcagcc gattttgagc attctcgtaa aggggcgagc ggctggtggg
     9601 agtggaagcc gcataaacga catctggaag gtttatttac tgccggaaag gtgatggtga
     9661 ttgaacggcg caacttccag cgcgtttatg atttaactca ccgtgtcatg cctgactggg
     9721 atgatgagcg cgatctcgtt tcgcaagcag aagcagaaat catcatgctg gataacagtg
     9781 cgcgtagcct gggaatattt cgcgaacagt ggctggcaga ttactaccgg ctaaaacgtc
     9841 cggcactggc ggcgtggcgt gaagcgaggg ctgaacagca gcaaatcatt gctgtgcatg
     9901 ttgaaaaatt gggcaatctt tggctgcatg ctgatttgct gccgctactc gagcgtgcgc
     9961 tcgccggaaa gctcactgca acgcacagcg cggtactttc gccttttgat cctgttgtct
    10021 gggatcgcaa acgcgcggag cagctttttg attttagcta ccggctggag tgctatatcc
    10081 cagcgccgaa acgccagtat ggctattatg ttctgccgtt attacatcgt gggcaattag
    10141 ttgggcgaat ggatgccaaa atgcatcgcc agacaggcat ccttgaagtt atctctctgt
    10201 ggttacagga aggcattaaa ccaacgacaa cgctgcaaaa agggttacgt caggcgatta
    10261 ctgatttcgc taactggcag caggcaacgc gggtgacatt aggacgctgc ccgcaaggcc
    10321 tctttactga ttgccgcgcc ggctgggaaa tagaccccgt cgcataaatg attatgataa
    10381 gatttatgca ttcataaccc gatccatctg gaggaactat ggatcatcgt ctgcttgaaa
    10441 tcattgcctg cccggtttgc aacggaaaac tttggtataa ccaggaaaaa caagagatta
    10501 tttgcaaact ggacaacctg gctttcccgc tgcgcgatgg cattccagtg ttgctggaaa
    10561 cggaagcccg cgtgctgact gctgatgaga gtaaatcatg agttttgtgg tcattattcc
    10621 cgcgcgttac gcgtccacgc gtctgcccgg taaaccattg gttgatatta acggcaaacc
    10681 catgattgtt catgttcttg aacgcgcgcg cgaatcaggt gccgagcgca tcatcgtggc
    10741 aaccgatcat gaggatgttg cccgcgctgt tgaagccgct ggcggcgaag tatgtatgac
    10801 gcgcgccgat catcagtcag gtacagaacg attggcggaa gttgtcgaaa aatgcgcatt
    10861 cagcgacgac actgtgatcg ttaatgtgca gggtgatgaa ccgatgatcc ctgcgacaat
    10921 cattcgtcag gttgctgata acctcgctca gcgtcaggtg ggtatggcga ctctggcggt
    10981 gccaatccac aatgcggaag aagcgtttaa cccgaatgcg gtgaaagtgg ttctcgacgc
    11041 cgaagggtac gcactgtact tctctcgcgc caccattccg tgggatcgtg atcgttttgc
    11101 aaaagacctt gaaaccgtag gcgataactt cctgcgtcat cttggtattt atggctaccg
    11161 agcaggcttt atccgtcgtt atgttacctg gcagccaagt ccgttagaac atatcgaaat
    11221 gttagagcag cttcgtgttc tgtggtacgg cgaaaaaatc catgttgctg tcgcccagga
    11281 agttcctggc acaggtgtgg atacccctga agatctcgag cgcgttcgcg ctgaaatgcg
    11341 ctaatttcac ttcacgacac tccagccaat tttgggagga gtgtcgtacc gttacgattt
    11401 tcctcaattt ttcttttcaa caattgatct cattcaggtg acatctttta tattggcgct
    11461 cattatgaaa gcagtagctt ttatgagggt aatctgaatg gaacagctgc gtgccgaatt
    11521 aagccattta ctgggcgaaa aactcagtcg tattgagtgc gtcaatgaaa aagcggatac
    11581 ggcgttgtgg gctttgtatg acagccaggg aaacccaatg ccgttaatgg caagaagctt
    11641 tagtacgccc ggaaaagccc gacaactggc atggaaaacc accatgctgg caagaagtgg
    11701 gactgtccgt atgccgacta tttatggtgt gatgacgcat gaagaacacc ccggccccga
    11761 tgtcctgcta ctggagcgaa tgcgtggtgt ttcggtggag gcaccagccc gaacaccaga
    11821 acgctgggaa caactcaaag atcaaatcgt tgaagcctta ctggcctggc accgtcagga
    11881 cagtcgcggt tgcgtcggcg cggtcgacaa tactcaggaa aatttctggc cctcatggta
    11941 ccggcaacat gttgaagtgc tatggaccac gctcaatcag ttcaataaca ccggtctaac
    12001 gatgcaggat aagcggatcc tgtttcgcac tcgcgaatgt ctcccggcat tatttgaagg
    12061 ctttaacgac aattgtgtac tgattcacgg taacttctgt ttacgtagca tgctgaaaga
    12121 ttcgcgcagc gatcagttac tggcgatggt cggaccggga ctaatgcttt gggcaccgcg
    12181 agaatacgaa ctgttccgac taatggataa ttctctggcg gaagatttgc tctggagtta
    12241 tctgcaacgc gcgccagtgg cggagtcgtt catctggcgg cgttggttgt atgtgttatg
    12301 ggatgaagtt gcgcaactgg tcaacaccgg acgatttagt cggcgtaact tcgatctggc
    12361 atcaaaatca ctcttgccgt ggctcgcctg acgaaccttt tagccactgc cagatgcggc
    12421 caagcgtttc atagccaaca cgatcgctat gcatcaacca aaccggagaa gggattgccc
    12481 gttcccacgg attaagcggc gagtcgatgg ccaactgatt cgccggggct ggcagcggat
    12541 ttagcccttc ctgctgaaaa aagatcatcg cccgcggcag atgtgatgcg gaggtcacca
    12601 gcaggaaagg ggcgtcacca attgcctgtt tcactgccgc agcttcttct tcggtatctt
    12661 ttggcaaatc cagagtgata atttgctcgc gcggcacgcc cagcgattgc gcaactcttg
    12721 cacctacttc ggctgtactc accgtattgg tttttgctac gcctcccgtg aagatcagtt
    12781 ttgatcccgg attttcgcgc cataagcgaa taccttcatt caggcggggc agactgttat
    12841 tgattaaatt cgagcttggt gcccactgcg ggttccaggt ataaccacct ccgagcacca
    12901 cgatatagtc cactttttgc gaattattcc atgtcggata ggtgctttcg atgggacgca
    12961 gcaggcggtc ggctaccggt tgcaaactca ataacaagag cgccagccac ccaatgctaa
    13021 taaaaatctt cccggttttt tgaaaacggc taaaccacag cagcgccagg ccagcgccaa
    13081 taatcaacaa catcagcgga agtggcagca acatattgcc aatcactttt ttcagtgtaa
    13141 aaagcatcct ttctggttcc ttttttgacc atatagcaag ggatcgccag cgatattaca
    13201 ccagacgggt tcattctcgc cgtggctgtg acaaaatagc gtctttatca gaaaacgtct
    13261 tagccaaccg gtggagaaag caatgcagga tcgcaatttt gatgatattg cggaaaagtt
    13321 ttcccgtaac atttacggca ccaccaaagg gcagcttcga caggctattc tgtggcagga
    13381 tctcgatcgc gtgctggcgg aaatgggccc gcaaaaactg cgtgtgctgg atgctggcgg
    13441 cggagaaggg cagaccgcaa tcaaaatggc cgagcgcggg catcaggtca ttttatgcga
    13501 tctttctgcg cagatgatcg accgcgcaaa acaggcggca gaagcaaaag gtgtgagcga
    13561 caacatgcaa tttatacatt gcgccgctca ggatgttgct tcgcatttgg aaacgcccgt
    13621 tgatctgata ttgttccatg cggtgctgga gtgggtggcc gatccccgca gcgtattaca
    13681 gaccctgtgg tcagttttgc gtccaggcgg cgtgttgtcg ttaatgttct acaatgcgca
    13741 tggtttgttg atgcataaca tggtcgccgg gaattttgat tacgtgcagg cgggaatgcc
    13801 gaaaaagaaa aaacggacgc tttcgccaga ttatccacgc gacccggcgc aggtttatct
    13861 gtggctggaa gaagctggtt ggcaaattat gggtaagaca ggcgttcgcg tgtttcatga
    13921 ttatctgcgc gagaagcacc agcagcgcga ctgctatgaa gcattacttg aattagaaac
    13981 gcgttattgc cgtcaggaac cgtatattac cctggggcgt tatattcatg tcaccgcgcg
    14041 caaaccgcag agcaaggata aagtatgagt gaattttccc agacagtccc cgaactggtt
    14101 gcctgggcca gaaaaaatga cttctccatc tcgctgccgg tagaccgact ctcttttctg
    14161 ctggcggttg ccacgctgaa cggcgagcgt ctggatggtg agatgagtga aggcgagctg
    14221 gtggatgcat tccgccatgt gagtgatgcg tttgagcaaa ccagcgaaac catcggcgtg
    14281 cgcgccaaca acgcgatcaa cgacatggtg cgtcaacgtc tgctgaaccg ctttaccagc
    14341 gagcaggcgg aagggaacgc aatttaccgt ctgacgccgc tcggcatcgg catcactgac
    14401 tattacattc gtcagcgcga gttttctacg ctgcgccttt ctatgcagtt gtcgattgtg
    14461 gcgggtgagc tcaaacgcgc agcagatgcc gccgaagagg gcggtgatga atttcactgg
    14521 caccgtaatg tttatgcgcc actgaaatat tcggtagcag aaattttcga cagtatcgac
    14581 ctgacgcaac gtctgatgga tgaacagcag cagcaggtga aggatgatat cgcccagttg
    14641 ttgaacaaag actggcgggc ggcgatttcc agctgtgaat tgttgctttc agaaacttcc
    14701 ggaacgctgc gtgaattgca ggatacgctg gaagcggcag gcgataaatt gcaggccaac
    14761 ctgttacgca ttcaggatgc gacgatgacc catgacgacc tgcattttgt cgatcgtctg
    14821 gtgttcgatc ttcagagcaa actcgaccgt attatcagtt ggggccagca atccatcgac
    14881 ttgtggattg gctacgaccg ccacgtacac aaatttattc gtactgcgat cgatatggat
    14941 aaaaaccgcg tctttgctca gcggttacgt cagtcggtac aaacctattt tgatgagccg
    15001 tgggcgctaa cctatgccaa tgccgatcgt ctgctggata tgcgtgacga agagatggta
    15061 ctgcgcgatg aagaagtgac tggggaactt cctgaggatc tggagtacga agagtttaac
    15121 gagatccgcg aacagctggc ggcgatcatc gaagaacaac ttgccgtgta caaaaccaga
    15181 caagtgccgc tggatcttgg tctggtggta cgcgaatatc tgtcacagta tccgcgtgca
    15241 cgtcactttg acgttgcgcg cattgttatt gatcaggcgg tacgtcttgg cgtagcgcaa
    15301 gcagatttca ccggactgcc agcgaaatgg cagccgatta atgattacgg agccaaggta
    15361 caggcgcatg tcatcgacaa atattgaaca agtgatgccg gttaagctgg cgcaggcgct
    15421 ggcgaatccg ttatttccgg cgctggatag cgccttacgt tcaggccgcc atattggcct
    15481 cgacgaactg gataatcatg cattcctgat ggattttcag gaatatctgg aagagtttta
    15541 cgcgcgttat aacgttgagc ttattcgtgc accggaaggg ttcttctatc tacgcccacg
    15601 ttccaccacg ctgatccctc gttccgtctt gtcggaactg gatatgatgg tcgggaaaat
    15661 cctctgttat ctctatctca gcccggaacg gctggcgaat gaggggattt ttactcaaca
    15721 ggaactgtac gacgaactgc tcaccctggc cgatgaagcg aaactgctga aactggtgaa
    15781 caaccgttca accggttcag acgttgaccg tcagaagttg caggaaaaag tacgctcttc
    15841 gctcaaccgt ctgcgtcgtt taggcatggt gtggtttatg ggccacgaca gcagcaagtt
    15901 ccgcattacc gaatcggtgt tccgcttcgg agccgatgtg cgtgctggcg acgatccccg
    15961 tgaagcacag cgtcgcctga ttcgtgatgg tgaagcaatg ccgattgaaa accatctgca
    16021 actcaacgat gaaaccgaag agagtcagcc agatagcgga gaggaagaat aatgattgaa
    16081 cgcggtaaat ttcgctcact gacgctgatt aactggaacg gcttttttgc ccgaactttt
    16141 gaccttgacg agctggtcac gacgctttct ggcggtaacg gggcgggtaa atccaccacc
    16201 atggcggcgt tcgttacggc gcttatcccc gatctgaccc tgctgcattt ccgtaacact
    16261 acggaagccg gggccaccag cggttcgcgc gataaaggtc tgcacggtaa gctgaaagcg
    16321 ggtgtctgtt attcgatgct tgacaccatt aactcgcgcc accagcgtgt agtggtcggt
    16381 gtgcgtctgc aacaggttgc cggacgcgat cgtaaagtgg atatcaaacc atttgccatt
    16441 cagggcctgc cgatgtcggt gcagccgaca caactggtga ccgaaactct gaacgaacgc
    16501 caggcgcgcg tgctgccgct taacgagctg aaagacaagc tcgaggcgat ggaaggcgtg
    16561 cagtttaaac agttcaactc cattaccgat taccactcgc tgatgttcga tctgggcatc
    16621 atcgcgcgtc gtctgcgctc agcgtctgac cgtagtaaat tctatcgtct gatcgaagcc
    16681 tcgctgtatg gcgggatctc cagcgccatt actcgttctc tgcgcgacta cctgttgccg
    16741 gaaaacagtg gggtgcggaa agcgttccag gacatggaag cggcgctgcg tgaaaaccgc
    16801 atgacgctgg aagcgattcg tgtcacccag tcggaccgag acctgtttaa gcatctgatc
    16861 agcgaagcca ccaactacgt ggcggcggac tacatgcgtc acgccaacga gcgccgtgtt
    16921 catctcgata aggccctgga gtttcgtcgc gagctacata cttcgcgtca gcaactggcg
    16981 gctgagcagt acaaacacgt cgatatggcg cgagagctgg ctgagcacaa cggtgccgaa
    17041 ggcgatctgg aagcggatta tcaggcggcc agcgatcacc tgaacctggt gcaaaccgca
    17101 ctgcgtcagc aggagaagat cgaacgctac gaagcggatc tcgatgagct gcagatccgt
    17161 ctggaagagc aaaacgaagt ggtggcagaa gccatcgaac gccaggaaga gaacgaggct
    17221 cgtgcggaag ctgccgaact ggaagtggac gagctgaaaa gccagcttgc tgactaccag
    17281 caggcgctgg acgtccagca aacgcgcgcg atccagtata accaggcgat tgctgcgctt
    17341 aatcgtgcca aagaactgtg ccatctgccg gacttaaccg ccgacagcgc tgccgaatgg
    17401 ctggaaacct tccaggcgaa agagctggaa gcgaccgaaa aaatgctctc tcttgagcag
    17461 aaaatgagca tggcgcaaac cgcgcacagc cagtttgagc aggcttatca gctggtggtg
    17521 gcaatcaacg gcccgctggc gcgtaacgag gcgtgggatg tcgctcgcga actattgcgc
    17581 gaaggggtcg atcagcgtca cctggcagag caggttcagc cgttgcggat gcgcttaagc
    17641 gaactggaac agcgtctgcg tgagcagcaa gaagctgagc gtctgctggc agatttctgc
    17701 aaacgtcagg gcaagaattt tgatattgat gaactggaag ctctgcatca ggagctggaa
    17761 gcacgcattg cctctctttc cgatagcgtc tctaacgccc gagaagagcg gatggcactg
    17821 cgccaggagc aggaacagct gcagtctcgc attcagagtt tgatgcagcg tgcgccggtt
    17881 tggctggcag cgcaaaacag tctcaaccag ttgagcgaac agtgcggcga agagttcacc
    17941 tccagccagg atgtcaccga atttctgcaa cagttgctgg agcgcgagcg cgaggcaatt
    18001 gttgaacgcg atgaagtagg cgcgcgcaaa aacgccgtcg atgaagagat cgaacgttta
    18061 agccagccag gtggttctga agatcagcgt ctgaacgcgc tggcggagcg ttttggtggt
    18121 gtgctgctgt cagaaattta tgacgacgtt agcctggaag atgcgccgta cttctcagca
    18181 ctgtatggcc cgtcacgcca cgccatcgtg gtgccagatc tgtcacaggt aaccgaacac
    18241 ctggaaggct tgaccgattg cccggaagat ctctatttga tcgaagggga tccgcagtca
    18301 ttcgatgaca gcgtgttcag cgttgatgag ctggaaaaag cggtggtggt gaaaatcgcc
    18361 gatcgtcagt ggcgttattc acgtttcccg gaagtgccgc tgtttggtcg tgctgcgcgt
    18421 gaaagccgta ttgaaagcct ccatgccgag cgggaagtgc tttccgaacg cttcgccacg
    18481 ctctcctttg atgtacagaa aacccagcgt ctgcatcagg cgttcagccg ctttatcggc
    18541 agtcatctgg cggtggcgtt tgagtctgac ccggaagcag aaatccgtca actgaacagc
    18601 cgtcgcgtcg aactggaacg ggcgttgagt aatcatgaaa atgataacca gcagcagcgt
    18661 attcagtttg agcaggcgaa agagggcgtt gcggcgctga accgcattct gccgcgtctc
    18721 aacctgttgg ctgatgacag cctggcggat cgcgtcgatg aaatccgcga acgtctggat
    18781 gaagcccagg aagccgcgcg ttttgttcag cagtttggca atcaactggc gaaactggaa
    18841 ccgatcgttt cggtattgca gagcgacccg gaacagttcg aacagttaaa agaagattac
    18901 gcgtactctc agcagatgca gcgcgatgcc cgtcagcagg cgtttgccct gacggaagtg
    18961 gtacagcgtc gtgcgcactt tagctactct gactcggcag aaatgcttag cggtaacagc
    19021 gatctcaacg aaaaactgcg tgaacgtctg gaacaggcgg aagcggagcg tacccgcgct
    19081 cgtgaagcgt tgcgcggaca cgcagcgcag ttgaatcagt acaaccaggt gctggcttcg
    19141 ctgaaaagtt cgtatgacac caaaaaagag ctacttaacg atctgcaacg tgaattgcag
    19201 gatatcggcg tgcgtgctga tagcggggca gaagagcggg cacgtattcg ccgtgacgag
    19261 ttgcacgcgc aactgagcaa taaccgttca cgacgcaatc aactggaaaa agcgcttacc
    19321 ttctgcgaag cggagatgga caacctgacc cgtaaactgc gcaagctgga gcgggattac
    19381 tttgagatgc gcgagcaggt agtgaccgcc aaagcgggct ggtgtgcggt gatgcgcatg
    19441 gtgaaagata atggcgttga gcgtcgctta caccgtcgtg agctggctta tctctccgct
    19501 gatgatttgc gttccatgtc ggataaggcg ttaggtgcgc tgcgtctggc ggtggcagat
    19561 aacgaacatc tgcgcgacgt gctgcggatg tcggaagatc caaaacgtcc ggaacgtaaa
    19621 attcagttct tcgtggcggt ttatcagcat ctgcgtgaac gtattcgtca ggatattatt
    19681 cgtaccgatg atccggtgga agctatcgaa cagatggaga tcgagcttag ccgtctgacc
    19741 gaagaattaa cctcccgtga acagaaactg gcgatcagtt cccgcagcgt ggcgaacatc
    19801 attcgcaaaa ccattcagcg cgagcagaac cgtatccgta tgctcaacca ggggttgcag
    19861 aacgtatcgt ttggccaggt gaacagcgta cgtctgaacg tgaacgtacg tgaaacgcac
    19921 gccatgctgc tggatgtgct ctctgaacag cacgagcagc atcaggatct gtttaacagc
    19981 aaccgcctga ccttctcaga agcgctggcg aaactgtatc aacgtcttaa cccgcagatt
    20041 gatatggggc agcgcacgcc gcagaccatc ggtgaagaac tgctggatta ccgcaactat
    20101 ctggaaatgg aagttgaggt taaccgtggt tccgatggct ggttgcgtgc agagtctggt
    20161 gcattgtcga ccggtgaggc gattggtacc ggtatgtcga ttctggtgat ggtggtacaa
    20221 agctgggaag atgaatctcg ccgcctgcgc ggtaaagata tctctccttg ccgcctgctg
    20281 ttcctcgatg aagcagcgcg actggacgcc cgttccatcg ccacgctgtt tgaattgtgt
    20341 gaacgtttgc aaatgcagct cattatcgca gcgccggaaa atatcagccc ggagaaaggc
    20401 accacctata aactggtgcg taaagtcttc cagaataccg aacacgttca cgtcgtcggc
    20461 ctgcgaggat ttgcgcctca actccctgaa acgcttccag gaagtgacga agcgccttct
    20521 caggcgagtt aaagaaaagg cggcagcaat gccgcctttt ctttttccga aaactccgtt
    20581 tctgcactaa aaaagtggca cattacggcg cgcttttctt taaacttctt tacattaggt
    20641 tatgtaaaaa cgtatcggcg tttatatact gaagataagt ctgatgagta acaggcttgc
    20701 tcgtcatact ttcgtgagta ttggcgttgt acaggcaagt cgtaaaataa cagcctggct
    20761 attcagagta tgataaaaac agggggcaag ggatgttgct taatatgatg tgtggtcgtc
    20821 ggctgtcggc aatcagtttg tgcctggccg taacattcgc tccactgttc aatgcgcagg
    20881 ccgatgagcc tgaagtaatc cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg
    20941 cactgccgca ggcgcaagcc acggcaataa tggcggggat ccagccattg cctgaaggtg
    21001 cggctgaaaa agcccgcacg caaatcgaat ctcaattacc cgcaggttat aagccggttt
    21061 atcttaacca gcttcaattg ttgtatgccg cacgcgatat gcaacccatg tgggaaaacc
    21121 gtgatgctgt taaagccttc cagcaacagc tggcagaggt ggcgattgcc ggtttccagc
    21181 cgcagtttaa taaatgggta gagttactga ccgatcctgg tgttaacggg atgacacgcg
    21241 acgtggtgct ctctgatgcg atgatgggct atctccattt cattgcaaat attccggtca
    21301 aaggcactcg ctggctatat agcagtaaac cttatgcgct tgcaacgccg ccgctttcgg
    21361 tgattaacca atggcagctg gcgctggata aaggtcaatt gcctacgttt gttgcaggac
    21421 tggcaccgca gcatccgcaa tatgcggcga tgcatgaatc gttactggcc ttactcagtg
    21481 acaccaaacc gtggccccaa ctgaccggca aagcaacgtt gcgcccagga cagtggagta
    21541 acgacgtacc ggcgttgcgc gaaatattgc aacgtacagg catgttggac gggggaccga
    21601 aaattacgct acctggcgat gacacgccaa ctgacgcggt agtcagccca tccgctgtta
    21661 ctattgaaac agcagaaact aagccgatgg acaagcaaac tacgtctcgt agtaaacctg
    21721 cgcctgtcgt tcgtgccgcc tacgataatg aactggtgga agccgttaaa cgttttcagg
    21781 catggcaagg attgggggca gatggtgcta ttggcccggc aacgcgtgac tggttaaacg
    21841 taacgcccgc ccagcgtgct ggcgtgttgg cactcaacat ccagcgattg cgcttgctgc
    21901 caacagagct ttctaccggg atcatggtta acattccggc ctattcgctg gtctactatc
    21961 agaacggcaa tcaggtgctg gattcgcgag tcattgtcgg tcgccccgat cgcaaaacgc
    22021 caatgatgag cagtgccctt aacaacgtag tggtaaaccc gccgtggaac gtaccaccaa
    22081 ctctggcacg caaagatatt ctgccgaaag tgcgcaacga tccgggatat ctcgaaagcc
    22141 atggctatac ggtgatgcgc ggctggaaca gcagagaagc gattgaccca tggcaggttg
    22201 actggtctac aatcacggcc tcgaatttac cgttccgctt ccagcaggct ccaggcccac
    22261 ggaactcgct ggggcgctat aaattcaata tgccgagttc agaggccatt tatttgcatg
    22321 acacgccgaa ccacaatctg ttcaagcgtg atacacgcgc attgagctca ggctgtgtac
    22381 gagtgaataa agcttccgat ctggcgaata tgctgttgca ggatgcaggc tggaatgaca
    22441 aacgtatttc tgatgcgctg aagcagggcg atacgcgtta cgtcaatatt cggcagtcga
    22501 ttccggtgaa tctctactat ctgacggcct ttgttggtgc agatggtcgt acccagtatc
    22561 gtacagatat ttacaattat gatctgcctg cgcgatccag ctcgcaaatt gtatcgaaag
    22621 cggaacaatt aatcaggtaa atgaagcagt tcggggaaat cagttgtcgt aataattagc
    22681 atgatcgggg gcgattctct gcagcccccg tcactgctgg ggttgagtca tcttgacgtc
    22741 tgctttacgg gcggttaagg tgcctcttgt gcgccagaag tgcatataaa cgataacatt
    22801 gacctgtaga cttgattatc atggacaaat tcgacgctaa tcgccgcaaa ctgctggcgc
    22861 ttggtggcgt tgcactcggt gccgccatcc tgccgacccc ggcgtttgca acactctcta
    22921 ccccacgccc gcgcattttg acactcaata atcttcatac cggagagtca atcaaagcgg
    22981 agtttttcga tggcagaggc tatattcagg aagaattggc aaaacttaac cattttttcc
    23041 gcgattaccg cgcgaacaaa ataaagtcca tcgacccagg attattcgac cagttgtatc
    23101 gcctgcaagg gttgttaggc acctgcaaac cggtgcaact catttccggt tatcgttcca
    23161 ttgataccaa caatgaacta cgcgcccgca gccgtggagt agcgaagaaa agctatcaca
    23221 ctaaaggcca ggcgatggat ttccacattg aaggtatcgc gttaagcaat attcgcaaag
    23281 ccgcgttatc tatgcgcgca ggtggtgtag gatattaccc acgtagtaac tttgtgcata
    23341 ttgataccgg gccagcacgg cactggtagt aatcgcttaa cgaaacaggg gcagtatgaa
    23401 ctatcgtatt attccggtca ccgcattctc ccagaactgt tcattgatct ggtgtgaaca
    23461 aacccgtctg gccgcactgg tcgatcctgg cggcgatgcg gaaaaaatca aacaggaagt
    23521 tgatgccagc ggcctgacac tgatgcagat cctgctgact catggtcatc tggaccacgt
    23581 tggcgcagcg gcggaactgg cgcaacatta cggcgtgccg gtttttggcc cggaaaaaga
    23641 agatgagttc tggctgcaag gcttgcctgc gcaaagtcgt atgtttggtc tggaagagtg
    23701 ccagccgctg acgccagatc gttggctgaa cgaaggcgat accatcagca tagggaatgt
    23761 gactttacag gtgttacatt gccctgggca tacgccgggt catgtcgtgt tttttgatga
    23821 tcgggcaaag ctgctgattt ctggcgacgt tattttcaaa ggtggagtag ggcgcagtga
    23881 cttcccgcgt ggcgatcata atcaactgat ttcttcaatc aaagataaat tgctgccact
    23941 gggggatgac gtgacattta ttccgggtca cggaccatta tccacacttg gttatgagcg
    24001 cctgcataat cccttcctgc aagacgaaat gcccgtctgg taaaggcgca taaaaaagcc
    24061 cgctttcaag cgggcttcat cggttttaac gcttacagca ctgccacaat cgcttcgcac
    24121 agcggcgcca tgttatctgg tgtcatcccg gccacgttta cgcgaccaga agcaacagca
    24181 tatacgccaa actcttcgcg cagacgcagc acttgttctt ttgtcaggcc actgaaggag
    24241 aacatgccgt tctgtttgat gataaagctg aagtcgcgat ttgcgccttt ttcctgcagc
    24301 gtattgacga acaactgacg catacgctga atacgctggc gcatatcagt cagttcttgt
    24361 tcccaaatcg cacgtaacgc atcgttgctc aggatggtgg caacaacaga agcgccgtgt
    24421 gctggtgggt tagagtagtt agcacgaatc gccgctttca tttggctgaa tgcgcgttca
    24481 acagtttcac tgtcagcagc aaccagagta caagcgccaa cacgctcgtt gtacaggcca
    24541 aagtttttag agtaggaact ggcaacaatc agctccttat gcagagccgc gaaagcgcgc
    24601 agtccttcag catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc
    24661 ggtaaccagc ctttctcaac ggagagttgt gccagtgtct gccattgttc cagcgtaggg
    24721 tcgatgccgg ttgggttatg gcagcagcca tggaacagca ctacgtcgcc agcctgggct
    24781 tcgttcaggc tgttaatcag tgcatcgaag tcaagagtgt gattttccgc atcataataa
    24841 gcgtattcac gaacttccag acctgcagag ttaaagacgc tcttatggtt cggccagctt
    24901 gggttgctca cccacacacg cttaacgctg gtattttttg ccaggaaatc ggcagccacg
    24961 cgtagtgcgc cagtgccacc cggagtctgt gccgtgcgag cacgtttgtc attgatcagg
    25021 gcgctacctt taccaaacag cagttcctga gtgcagcgac caaattcagg gatgccgtca
    25081 atgccgaggt aatttttggt ggtttcattt tcgagcagat actgttcagc ctttttcacg
    25141 ctggtcagta ccggggtttt acccgtctca tctttataga caccaatccc gaggttaatt
    25201 ttgccgggac gttcatcggc acgaaacaga tcggccaggc ccagaatcgg gtcggcagga
    25261 gcggcggtaa tgttctcaaa catgacgagg ttccattatg gttacagaag ggaagtccgc
    25321 tatcagggta acgggagatt tacaaaattc caactattac tgatgaaaac gcaggctgtt
    25381 tttgcaagac gtgagattgc tctggaaggc ataaaaaaaa caggaccaaa gtcctgtttt
    25441 ttcggcattt aacaaagagg tgtgctatta gaactggtaa acgataccca cagcaacggt
    25501 gtcgtcagaa cctacgccca gtttgttgtc agaatcgatc tggttgatga tgtagtcaac
    25561 ataggtggac atgtttttgt tgaaatagta ggttgcgccc acttcaaagt agttcaccag
    25621 atcaacatca ccgatacctt ctacgtcttt cgctttagat ttggtgtaag cgatggacgg
    25681 acgcagaccg aaatcaaact ggtattgcgc aactaacaga acgtcttgcg ttttgttggc
    25741 gaagccgctg gtgtttgtaa atttattagt gatcggcgta gcgttacggg tttcaccgta
    25801 gttcgctgcc aggtagatgt tgttcgcgtc atacttcagg ccagtagccc actgttcagc
    25861 ttttttgcct ttgccaaggg aagactcttc ctgcaggttg gtacggtcag cggcaccata
    25921 agcaccaacg ataccaaagc cttcgtattc gtagctgata gaaccgccaa caccgtcgcc
    25981 gttagagcgg cgtgcagtgt cacgctcgtt tttacccagg tactgaacag cgaagttcag
    26041 accatcaacc agaccaaaga agttggagtt acgataggta gcaacgccgc caacacgacc
    26101 aacgaagaaa tcatcgctgt atgcagtatc accaccaaat tctggcagca tatcggtgta
    26161 acccagtgca tcataaacca caccgtagtt acggccgtaa tcgaaagaac caacgtcagc
    26221 gtatttaaga cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt cagcgccttc
    26281 agagttgtta ccctggaagt tatattccca ctgaccataa ccggtcagat cggaattgat
    26341 ttgagtttcc cctttaaaac caagacgggc ataggtcatg tcgccattgc caccgtaact
    26401 gttttcaccg ttacccttgg agaaataatg cagaccgaca gctttaccgt acagatctac
    26461 tttgttgcca tctttgttat agatttctgc agcgtttgca gtacctgcta ctaacagagc
    26521 agggacgatc actgccagaa tattgcgctt catcattatt tattaccctc atggtttttt
    26581 tatgacacct gccactgccg tcaataagtt ctgtcaataa aaatttacgg aactattgat
    26641 gagagtttgg tgtctttatg tgtctgcagg catctttcca ttcaaa
//
LOCUS       DAZFRG010000041        26565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFRG010000041 DAZFRG010000000
VERSION     DAZFRG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.041"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..705
                     /gene="bcsQ"
                     /locus_tag="ACHKUC_001364"
     CDS             <1..705
                     /gene="bcsQ"
                     /locus_tag="ACHKUC_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO6617649.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEFRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
     gene            702..3320
                     /gene="bcsA"
                     /locus_tag="ACHKUC_001365"
                     /pseudo
     CDS             702..3320
                     /gene="bcsA"
                     /locus_tag="ACHKUC_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3331..5670
                     /gene="bcsB"
                     /locus_tag="ACHKUC_001366"
     CDS             3331..5670
                     /gene="bcsB"
                     /locus_tag="ACHKUC_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO6617650.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPSPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5677..6783
                     /gene="bcsZ"
                     /locus_tag="ACHKUC_001367"
     CDS             5677..6783
                     /gene="bcsZ"
                     /locus_tag="ACHKUC_001367"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO6617651.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVPTGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6765..10238
                     /gene="bcsC"
                     /locus_tag="ACHKUC_001368"
     CDS             6765..10238
                     /gene="bcsC"
                     /locus_tag="ACHKUC_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO6617652.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVVNLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRGQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10320..12308
                     /gene="hmsP"
                     /locus_tag="ACHKUC_001369"
     CDS             10320..12308
                     /gene="hmsP"
                     /locus_tag="ACHKUC_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO6617653.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12491..13777
                     /gene="dctA"
                     /locus_tag="ACHKUC_001370"
     CDS             12491..13777
                     /gene="dctA"
                     /locus_tag="ACHKUC_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO6617654.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13997..15493
                     /gene="yhjJ"
                     /locus_tag="ACHKUC_001371"
     CDS             13997..15493
                     /gene="yhjJ"
                     /locus_tag="ACHKUC_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6617655.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15589..16518)
                     /gene="kdgK"
                     /locus_tag="ACHKUC_001372"
     CDS             complement(15589..16518)
                     /gene="kdgK"
                     /locus_tag="ACHKUC_001372"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO6617656.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGIAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16750..17517
                     /gene="pdeH"
                     /locus_tag="ACHKUC_001373"
     CDS             16750..17517
                     /gene="pdeH"
                     /locus_tag="ACHKUC_001373"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO6617657.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17587..19647
                     /gene="yhjG"
                     /locus_tag="ACHKUC_001374"
     CDS             17587..19647
                     /gene="yhjG"
                     /locus_tag="ACHKUC_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6617658.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVIGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQVFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19826..21148)
                     /gene="yhjE"
                     /locus_tag="ACHKUC_001375"
     CDS             complement(19826..21148)
                     /gene="yhjE"
                     /locus_tag="ACHKUC_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6617659.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21541..22554)
                     /gene="yhjD"
                     /locus_tag="ACHKUC_001376"
     CDS             complement(21541..22554)
                     /gene="yhjD"
                     /locus_tag="ACHKUC_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO6617660.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22603..23502)
                     /gene="rcdB"
                     /locus_tag="ACHKUC_001377"
     CDS             complement(22603..23502)
                     /gene="rcdB"
                     /locus_tag="ACHKUC_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6617661.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGIAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            24022..24624
                     /gene="yhjB"
                     /locus_tag="ACHKUC_001378"
     CDS             24022..24624
                     /gene="yhjB"
                     /locus_tag="ACHKUC_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6617662.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24675..26324)
                     /gene="treF"
                     /locus_tag="ACHKUC_001379"
     CDS             complement(24675..26324)
                     /gene="treF"
                     /locus_tag="ACHKUC_001379"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6617663.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTHEPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKDEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         6224 a         6771 c         7363 g         6207 t
ORIGIN      
        1 accatcaccg ccgcattagc ctggtcatta caaatgttgg gagaaaatgt cctggtggtc
       61 gatgcctgcc cggacaactt gttgcgcctg tcatttaacg ttgattttac ccaccgtcag
      121 ggttgggcaa gagctatgct ggatggccag gactggcgtg acgccgggtt gcgctatacc
      181 tcgcagctcg atttgctgcc ttttggtcag ttatccattg aagaacaaga aaatccacag
      241 cactggcaaa cccggctgag cgatatttgc tccggcttac agcaactaaa agccagcggg
      301 cgttaccagt ggattttaat tgacttaccg cgtgatgcct cgcagataac ccaccagctg
      361 ctgagtttgt gcgatcactc gctggcaatc gtcaatgtgg atgccaactg ccatatccga
      421 ctgcatcagc aagcgctgcc ggatggtgca catattttga ttaatgactt ccgtattggc
      481 agccaggttc aggacgatat ttaccagctt tggttgcaaa gccagcgccg attgctgccg
      541 atgctcattc atcgtgatga agcgatggct gaatgtctgg cagctaagca accagtcggt
      601 gaatttcgca gtgatgcgct ggcggctgaa gaaatactga cgctggcgaa ctggtgcctg
      661 ttgaactatt ccgggctgaa aacgccagtc gggagtgcat catgagtatc ctgacccggt
      721 ggttgcttat cccgccggtc aacgcacggc ttattgggcg ttatcgcgat tatcgtcgtc
      781 acggcgcgtc ggctttcagc gcgacgctcg gctgtttctg gatgatcctg gcctggattt
      841 ttattccgct ggagcacccg cgctggcagc gtattcgcgc agaacataaa aacctgtatc
      901 cgcatatcaa cgcctcgcgt ccgcgtccgc tggacccggt ccgttatctt attcaaacat
      961 gctggttact gatcggtgca tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt
     1021 caggtctaca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     1081 ttagccataa aacacagcat cttgatgaga aaaaagagct cggtcatttg agtgccgggg
     1141 cgcggcggtt gatccttggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     1201 ttactcagcc gtttaacccg ctagcgcagt ttatcttcct gatgctgctg tggggggtag
     1261 cgctgatcgt acggcggatg ccggggcgct tctcggcgct gatgctgatt gtgctgtcgc
     1321 tgaccgtttc ttgccgttat atctggtggc gttacacctc gacgttaaac tgggacgatc
     1381 cggtcagcct ggtgtgcggg cttattctgc tgttcgccga aacgtacgcg tggattgtgc
     1441 tggtgctcgg ctatttccag gtcgtatggc ctctgaatcg ttagccggtg ccattgccga
     1501 aagatatgtc gctgtggccg tcggtggata tctttgtccc gacttacaac gaagatctca
     1561 acgtggtgaa aaataccatt tacgcttcgc tgggtatcga ctggccgaaa gacaagctga
     1621 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     1681 gggtgaaata tatcgcccgt accactcatg aacatgcgaa agcgggcaac atcaacaatg
     1741 cgctgaaata tgccaaaggc gagttcgtgt cgatattcga ctgcgaccac gtaccaacgc
     1801 gatcgttcct gcaaatgacc atgggatggt tcctgaaaga gaaacagctg gcgatgatgc
     1861 agacgccgca ccacttcttc tcgccggatc cgtttgaacg caacctgggg cgtttccgta
     1921 aaacaccgaa cgaaggcacg ctgttctatg gtctggtgca ggacggcaat gatatgtggg
     1981 atgccacttt cttctgcggc tcatgtgcgg tgattcgccg taagccgctg gatgagattg
     2041 gcggtattgc tgtcgaaacg gtaaccgaag atgcgcacac ttctctgcgt ctgcaccgtc
     2101 gtggctacac ctccgcgtat atgcgtattc cgcaggcagc ggggctggcg accgaaagtc
     2161 tctcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtgcaa atcttccgtc
     2221 tcgataaccc gctcaccggt aaagggctga agtttgctca gcgactgtgc tatgtcaacg
     2281 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     2341 tcctgctgtt tcatgcctac atcatctacg cgccagcgtt gatgatcgcc ctgtttgtgc
     2401 taccgcatat gatccatgcc agcctgacta actcgaagat ccagggcaaa tatcgccact
     2461 ctttctggag tgaaatctac gaaacagtgc tggcgtggta tatcgcacca ccgacgctgg
     2521 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     2581 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctgctcaacc
     2641 tggtgggcgt agcggttggg atctggcgct acttctatgg tccgccaacc gagatgctca
     2701 ccgtggtcgt cagtatggtg tgggtattct acaacctgat tattctcggc ggcgcagtcg
     2761 cagtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     2821 cagcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     2881 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     2941 tgttgcttaa acgcggtcag caggaatacg tcttcccggc tcaggtggcg cgcgtgatgg
     3001 gtaatgaagt tgggctgaaa ttaatgccgc tcactaccca gcaacatatc gattttgtgc
     3061 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     3121 agccactgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     3181 cggaatttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     3241 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     3301 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aaatattctg gatttgtgca
     3361 gtggctctgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     3421 ccactgatca atgctgagcc agctgtagcc gcccaggcgg agcaaaatcc gcaggtgggg
     3481 caagtgatgc cgggcgtgca gggcgcagat gcgccagtcg tggcgcagaa cggtccttcc
     3541 cgtgatgtga agctgacctt tgcgcaaatt gcgccgtcgc cgggcagcat ggtgctacgt
     3601 ggcattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     3661 gcgatgctca acctcgaata cacgccatcg ccatcattac tgcctgtcca gtcgcagtta
     3721 aaggtttatc tcaatgatga actgatgggc gtgctgccag tgaccaaaga acagttgggt
     3781 aaaaaaacgc tggcacaaat gcccattaac ccgctgttta ttactgattt caaccgtgtg
     3841 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     3901 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     3961 aatgacctgt cacacttccc ggtgccattc tttgacccgc gtgataaccg caccaacacc
     4021 ttgccgatgg tctttgcggg tgcgccggat gttgagctgc aacaagcatc tgccattgtt
     4081 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     4141 caactgccgg atcgcaatgc cattgtcttt gccaccaatg acaaacgccc ggacttcctg
     4201 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     4261 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acttgttgca ggcagcgaaa
     4321 ggtatcgctc agggtaacat tctgttccgt ggtgaaagtg tggtagtgaa tgaagtgaaa
     4381 ccgctgctac cgcgtaaacc gtacgatgcg ccgaactggg tgcgtaccga tcgtccggtc
     4441 acctttggtg aactgaaaac ctatgaagaa caattacaat ccagcggtct tgagccagca
     4501 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     4561 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     4621 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     4681 cgcctgctgc tgcggattcc ggtattacaa ggtttgctgg atggcaaaac ggatgtctct
     4741 attccggcgc tgaaactggg cgcgaccaac cagctgcggt tcgactttga gtatatgaac
     4801 ccgatgccgg gcggttcggt ggataactgc attaccttcc agccggtgca gaatcatgtg
     4861 gtgattggtg acgactccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     4921 gatctacgcg cctttgctaa cgcgggcttc ccgttcagcc ggatggcgga tttgtcgcaa
     4981 accatcaccg tgatgccgaa aacgccaaac gaagcacaga tggaaacctt gctgaatacc
     5041 gttggcttta tcggtgcgca gacgggcttc ccggcgatta acctgacggt gaccgatgat
     5101 ggcagcacca ttcagggcaa agatgccgac atcatgatcg tcggtggtat cccggacaaa
     5161 ctgaaagacg ataagcagat cgacctgttg gtgcaggcta ccgaaagctg ggtgaaaacg
     5221 ccgatgcgcc agaccccgtt ccccggtatt gtgccggacg agagcgatcg cgcggcagaa
     5281 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggttt ccagtcgccg
     5341 tataacgacc agcgcagcgt gattgcgctg ctggcagata gcccacgcgg ttatgaaatg
     5401 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     5461 atccgcgagt ccggtatcaa cagcctacgt gttggcgatg tttattacgt aggtcatctg
     5521 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     5581 gcggctatca gtgtgatatt gctggcgtgg gtactgtggc gtctgctgcg tattattagt
     5641 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggactc
     5701 gtgacgatgc tgctgctggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     5761 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     5821 cgcaaaatca ccacctccga agggcaaagt tacggcatgt tctttgccct ggcggctaac
     5881 gaccgtgcag ctttcgataa tcttctcgac tggacgcaga acaatctcgc tcagggttct
     5941 ttaaaagaac atttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6001 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     6061 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcattgct aaaacgtatc
     6121 gcgcgggagg aagtggtgac ggtgcctggg ctgggctcca tgttgttacc gggcaaagtg
     6181 ggttttgctg aggataacag ctggcgtttt aaccccagct acctgccgcc gacgctggcg
     6241 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacgttta
     6301 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     6361 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     6421 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     6481 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtgcct
     6541 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcccttttta
     6601 caaaaccgcg atgcgcaggc cgttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     6661 gatgcctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     6721 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     6781 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     6841 ccgctcagca acagttgctg gagcaagttc ggttaggcga agcgacccat cgtgaagacc
     6901 tggtgcaaca gtcgttgtat cggctggaac ttattgatcc gaataacccg gatgtgattg
     6961 ctgcccgttt tcgttctctg ttacgtcagg gcgatattga tggcgcacaa aaacagctcg
     7021 atcggctgtc gcagttagcg ccgagttcaa atgcgtataa atcgtcgcgg accacaatgt
     7081 tactgtctac gccggatggt cgtcaggcac tgcaacaggc tcgattgcag gcgactaccg
     7141 gtcatgcgga agaggccgtg gcgagttaca acaagctgtt caacggtgcg ccgccggaag
     7201 gtgacattgc tgtcgagtac tggagtacgg tggcgaaaat tccggctcgc cgtggtgaag
     7261 cgattaatca gctaaaacgc attaatgcgg atacgccggg caatacgggc ctgcaaaaca
     7321 atctggcgct attactgttt agtagcgatc gccgtgacga aggttttgcc gtcctggaac
     7381 agatggcgaa atcgaacgcc gggcgcgaag gggcctctaa aatctggtac gggcagatta
     7441 aagacatgcc cgtcagcgat gccagcgttc aggcactgaa aaaatatctc tcgatcttta
     7501 gcgatggcga tagcgtggct gctgcacaat cgcaactggc agaacagcaa aaacagctgg
     7561 ccgaccctgc tttccgcgct cgtgcgcaag gtttagcggc ggtggactct ggcatggcgg
     7621 gtaaagccat tcccgaacta caacaggcgg tgcgggcgaa cccgaaagac agtgaggctc
     7681 tgggggcgct gggccaggcg tattcccaga aaggcgatcg cgccaatgca gtggtgaatc
     7741 tggaaaaagc cctcgcgctg gatccgcaca acagcaataa cgacaaatgg aacagcctgc
     7801 tgaaagtaaa ccgctactgg ctggcgatcc agcagggcga tgccgcgctg aaagccaata
     7861 atcctgaccg ggcagaacgg ctgttccagc aggcacgtaa tgtcgataac accgacagct
     7921 atgcagtgct tgggctgggc gatgtggcga tggcgcgcaa agattatccc gccgccgaac
     7981 gctattacca gcagaccttg cgtatggaca gcggcaacac taacgccgtg cgcgggctgg
     8041 cgaatattta ccgccagcag tcgccagaaa aagccgaagc gtatatcgcc tcgctctctg
     8101 ccagccagcg gcgcagcatt gatgatatcg aacgcagcct gcaaaacgac cgtctggcac
     8161 agcaggcaga ggcactggaa aaccagggga aatgggcgca ggcggcagca cttcagcggc
     8221 aacgactggc gctggacccc ggcagtgtat ggattactta ccgactttcg caggatctct
     8281 ggcaagccgg acaacgcagc caggccgata cgttaatgcg caatctggcg cagcagaagc
     8341 cgaacgaccc ggagcaggtt tacgcttacg ggctgtacct ctccggtcac gaccaggaca
     8401 gagcggcgct ggcgcatatc aacagcctgc cgcgcgggca gtggaacagc aatattcagg
     8461 agctggttaa tcgactgcaa aacgatcagg tgctggaaac cgctaatcgc ctgcgagaaa
     8521 acggtaaaga ggcagaagcg gaagcgatgc tgcgccagca accgccttcc tcgcgtattg
     8581 acctcacgct ggctgactgg gcgcagcagc gacgtgatta caccgccgcc cgcgctgcat
     8641 atcagaatgt cctgacgcgg gagccaacta acgccgatgc cattcttggt ctgacggaag
     8701 tggatattgc tgccggtgac aaagcggcgg cacgtagcca gctggcgaaa ctgcctgcca
     8761 ctgataacgc ctcgctgaac acacagcggc gcgtggcgct ggcacaggcg cagcttggcg
     8821 ataccgcagc ggcgcagcag acatttaata agttgatccc gcaggcaaaa tctcagccac
     8881 cgtcgatgga aagcgcgatg gtactgcgtg atggtgcgaa gtttgaagcg caggcgggcg
     8941 atccaacgca ggcgctggaa acctacaaag acgccatggt tgcatccggt gtgaccacga
     9001 cgcgtccgca ggataacgac acttttaccc ggctgacccg taacgacgag aaagatgact
     9061 ggctgaaacg cggcgtgcgc agcgatgcgg cggacctcta tcgccagcag gatcttaacg
     9121 tcactctcga gcacgattac tggggttcga gcggcaccgg tggttactcc gatctgaaag
     9181 cgcacaccac tatgttgcag gtggacgcgc cctattctga cgggcggatg ttctttcgca
     9241 gtgatttcgt caatatgaac gtcggcagtt tctccactaa tgccgatggc aaatgggatg
     9301 acaactgggg cacctgtaca ttacaggact gtagcggcaa ccgcagccag tcggattccg
     9361 gtgccagcgt ggcggtcggc tggcgaaatg acgtctggag ctgggatatc ggtaccacgc
     9421 cgatgggctt caacgtggtg gatgtggtcg gcggcatcag ttacagcgat gatatcgggc
     9481 cgctgggtta caccgttaac gcccaccgtc ggcctatctc cagttctttg ctggcctttg
     9541 gtgggcaaaa agactctccg agcaataccg ggaaaaaatg gggcggcgtg cgtgccgacg
     9601 gcgtggggct aagtctgagc tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta
     9661 gcggtgatca gttaaccggt aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg
     9721 gctattacta taaggtcatc aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca
     9781 tgatctggca ttatgacaaa gacctgagtg gctactcact cggtcagggc ggttactaca
     9841 gtccgcagga atacctgtcg tttgccatac cggtgatgtg gcgggagcgc acggaaaact
     9901 ggtcgtggga gctgggggcg tctggctcgt ggtcgcattc acgcaccaaa accatgccgc
     9961 gttatccgct gatgaatctg atcccgaccg actggcagga agaagctgcg cggcaatcca
    10021 acgatggcgg cagcagtcag ggctttggct acacggcgcg ggcattactt gaacgacgtg
    10081 ttacttccaa ctggtttgtc ggcacggcaa ttgatatcca gcaggcaaaa gattacgccc
    10141 ccagccattt cctgctctac gtacgttatt ccgccgccgg atggcagggt gacatggatt
    10201 taccgccgca gccgctgata ccttacgccg actggtaagt tttcagatag cgcctctctt
    10261 aatgccgctg cgatcgggta tactcgggcg gcaatctggg atttccgggg ggagacaatt
    10321 tgcgcgtaag tcgctcgtta acaatcaagc agatggcaat ggtggcagcc gttgtcctgg
    10381 tgttcgtttt tattttttgc accgttttgc tgttccatct ggtccagcag aatcgctata
    10441 acacggctac gcaactggaa agcattgctc gctctgtccg cgaaccctta tcttctgcca
    10501 ttttgaaagg cgatattccc gaagcggaag ctattcttgc cagcattaaa ccggcaggcg
    10561 tggtcagccg tgccgatgta gtgctgccta accagttcca ggcgctgcgt aaaagtttta
    10621 ttccagagcg tccggtgccg gtaatggtta ctcgcctgtt tgagctaccg gtgcaaatct
    10681 cgctgggcgt ttactcgctg gaacgtccgg caaacccgca gccaattgcc tatctggtgt
    10741 tacaggcgga ttccttccgc atgtataagt tcgtgatgag caccctctca acgttagtga
    10801 ccatttactt actcttgtcg ttaatattga cggtggcgat tagctggtgc attaaccgcc
    10861 tgattttgca tccgttacgc aatattgctc gcgaacttaa cgccatccca ccccaggagc
    10921 ttgttggtca ccaactggca ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg
    10981 tgcgcagtta caacctcaac cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa
    11041 atgcgatgcg cttcccggtg tcggatttgc cgaacaaagc cttgctgatg gagatgctgg
    11101 agcaggtggt ggcgcgtaaa caaaccaccg cgctgatgat catcacctgt gaaaccctgc
    11161 gtgatactgc gggtgtgctg aaagaggcgc aacgagaaat tctgctgctg acgctggtgg
    11221 aaaaactcaa atcggtactg tcgccacgca tgatcctcgc gcagattagc ggttatgact
    11281 ttgctgtcat tgccaacggt gtacaggaac cgtggcacgc tatcacatta ggtcagcaag
    11341 tgctcactat catgagcgag cgcctgccga ttgaacgtat tcaactccgt ccgcactgta
    11401 gcattggcgt ggcgatgttc tacggcgatc tcaccgccga acagctttac agtcgcgcta
    11461 tttctgcggc atttaccgct cgccataaag gcaaaaatca gattcagttc tttgatccgc
    11521 agcagatgga agccgcccag aagcggttga cggaagagag cgatatcctt aatgcactgg
    11581 aaaatcatca gtttgcgatt tggttacagc cacaggtcga gatgaccagc ggtaaactgg
    11641 tcagtgcgga agtgttactg cgtatccagc aaccggatgg cagttgggac ctgcccgatg
    11701 gcttaatcga tcgcattgag tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg
    11761 aagagtcctg tcgattgctt gctgcctggc aagagcgcgg cattatgctg cccttgtcgg
    11821 taaacctctc agcgctgcaa ctgatgcatc caaatatggt ggcggatatg ctggaattgt
    11881 taacccgcta tcgtattcag ccgggaacac tgattctgga agtgacagaa agccgacgta
    11941 ttgacgatcc tcatgctgcg gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg
    12001 tggcgctgga tgatttcggc atgggctacg cagggctgcg tcagctgcag catatgaaat
    12061 cgttgccaat cgacgtactg aaaatcgaca aaatgtttgt tgaaggtttg ccggaagata
    12121 gcagcatgat tgctgcaatt atcatgctgg cgcagagcct gaacctacaa atgattgccg
    12181 aaggcgtgga gactgaagca caacgcgact ggctggcaaa agcgggcgtt ggtattgccc
    12241 agggcttcct ttttgctcgc ccactcccta ttgaaatctt cgaagagagt tacctggaag
    12301 aaaagtagct accccaaact gattacaaaa ctttaaaaag tgctggtttg tgcgagccag
    12361 ctcaaacttt ttaacctttt tgtttcaatt atgatccagg tacatttctg tgatgttgtc
    12421 tgggtgttat tttaaggccg caggtacccc ataaccttac aagacctgtg gttttactaa
    12481 aggacaccct atgaaaacct ctctgtttaa aagcctttac tttcaggtcc tgacagcgat
    12541 agccattggt attctccttg gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct
    12601 tggcgacggc ttcgttaagc tcattaagat gatcatcgct cctgtcatct tttgtaccgt
    12661 cgtaacgggc attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc
    12721 actgctttac tttgaaattg tcagtaccat cgcgctgatt attggtctta tcatcgttaa
    12781 cgtcgtgcag cctggtgccg gaatgaacgt cgatccggca acgcttgatg cgaaagcggt
    12841 agcggtttac gccgatcagg cgaaagacca gggcattgtc gccttcatta tggatgtcat
    12901 cccggcgagc gtcattggcg catttgccag cggtaacatt ctgcaggtgc tgctgtttgc
    12961 cgtactgttt ggttttgcgc tccaccgtct gggcagcaaa ggccaactga tttttaacgt
    13021 gatcgaaagt ttctcgcagg tcatcttcgg catcatcaat atgatcatgc gtctggcacc
    13081 tattggtgcg ttcggggcaa tggcgtttac catcggtaaa tacggcgtcg gcacactggt
    13141 gcaactgggg cagctgatta tctgcttcta catcacctgt atcctgtttg tggtgctggt
    13201 actgggttca atcgctaaag cgactggttt cagtatcttc aaatttatcc gttacatccg
    13261 tgaagaactg ctgattgtac tggggacttc atcttccgag tcggcgctgc cgcgtatgct
    13321 cgacaagatg gagaaactcg gctgccgcaa atcggtggtg gggctggtca tcccgacagg
    13381 ctactcgttt aatcttgatg gcacatcgat atacctgaca atggcggcgg tgtttatcgc
    13441 ccaggccact aacagtcaga tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct
    13501 gctttcttct aaaggggcgg caggggtaac gggtagtggt tttatcgtgc tggcggcgac
    13561 gctctctgcg gtgggccatt tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg
    13621 ctttatgtca gaagctcgtg cgctgactaa cctggtcggt aacggcgtag cgaccattgt
    13681 cgttgctaag tgggtgaaag aactggacca caaaaaactg gacgatgtgc tgaataatcg
    13741 tgcgccggat ggcaaaacgc acgaattatc ctcttaatct caccacttat gcccgcactc
    13801 ccttccccga gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact taccacctgg
    13861 gtgagttcag gtttatttca cttcttccgt gacattttca tgttcttgcg gtctaacacg
    13921 aagtgttttt aacgtcattt agactgaccg acaacgggca gttttttatt accaggatag
    13981 ttgatcaggg gttcacatgc agggcacaaa aattcgactt ttagcgggcg gtttgctgat
    14041 gatggccact gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca
    14101 ggggacgctt tccaacggtt tacagtggca agtgctgact acgccccagc gtcccagcga
    14161 tcgtgttgaa attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag
    14221 cggttacagt cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc
    14281 gcaggcgcgt tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat
    14341 tgtctcttat gacaccacgc tgtttaatct gagtttgccc aataaccgta acgacttgct
    14401 gaaagaggcg ctctcttatc tggcaaatgc cactggcaaa ctgactatca cgccagaaac
    14461 catcaaccat gcgttgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga
    14521 gggctggtgg cgttatcgtc tgaaaggatc aaccttgtta ggtcacgatc ctgccgatcc
    14581 gctgaaacaa cccgttgaag cggaaaagat taaagatttc tatcagaaat ggtacacccc
    14641 ggatgcaatg acgctgctgg tggtgggaaa cgtggatgcg cgctcggtcg tcgaccaaat
    14701 caataaaacg tttggcgaac tgaaaggcaa acgtgaaaca ccggctccgg tgccgacgct
    14761 ttctccgctg cgtgcggaag cggtgagtat tatgaccgac gcggtgcgtc aggaccggct
    14821 atctatcatg tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta
    14881 ctggcgtgcg gacctggccc gtgaggcgct gttctggcat gttcagcaag cgttaagcgc
    14941 cagtaacagt aaagacatcg gtcttggatt tgactgtcgt gtgctgtatc tgcgtgcgca
    15001 gtgtgccatc aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc
    15061 gcgtgaactg gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt
    15121 ggcgcaaaag aaactggagc tgcagaaact gtttgccgcc tatgcgcgag ctgataccga
    15181 tattctgatg gggcagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga
    15241 gcaatatcag aaactgcgtc aggatttcct taacagcctg acggtggaga tgttaaatca
    15301 ggatctgcgt cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga
    15361 gccggaattt aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatcgac
    15421 tgcggctgcc gccacatctg tcgccacgga tgacgtacat cctgaagtga cggatattcc
    15481 acctgtacag taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga
    15541 gctggatagg ggggtaacac tctatccagc cttttgcata tgctgctttt acgctggcat
    15601 cgcttcacgc gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc
    15661 gcgtttcgct gcgtcttccg caattccgcc cgtcaaacgt accgccagat aaccggcact
    15721 gaaagagtcg ccagctgcgg tggtatcgat cactttttct ttcggcagtt ttaccgccgg
    15781 aacatccact aacccttcgc cagcaatgga caccaggcaa gaatccgccc cgcgtttcac
    15841 caccacttct ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg
    15901 accccacagc gcgtcttcat cgtccagcgt caggaaggcg atatccgtgc attccagcat
    15961 ttgttggtac acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt
    16021 gtcgaaaatc acttttccgc cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc
    16081 gcggctggtc gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt
    16141 cgccagctct tcgcaaatcg ccgcggattg ctcactctcc agccagaatt tggcggcggc
    16201 ttcgttacgc cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag
    16261 gcccggcaga cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgcgcca
    16321 ggcgtccagc atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt
    16381 taatgccgca ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc
    16441 gaaaccgcgc ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac
    16501 ggcaatcttt ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc
    16561 tgtggtggtt tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa
    16621 cgtattcctg aaagattccg ttgtggagtg gggaaattta ccccgtccag gcggtaaagt
    16681 tctgcccttg cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg
    16741 acatctttga tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag
    16801 agcttgcagg aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc
    16861 tatcaaacat gcgggcggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg
    16921 aacccttcgc aacgcctgcc gccggatcgc tattttactg aaatcaccgt tagccatcgg
    16981 atggaggttg tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttgtagag
    17041 cacggcctgc tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa
    17101 ccaaaaatcc tgcgccagat tgaggatctt ccctggctgc gtttcgaact ggtggaacac
    17161 atccgcctgc cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg
    17221 gatgattttg gtaccgggat ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac
    17281 atcaaaatcg cgcgagaact gtttgtgatg ctgcgtcagt caccggaagg acgcacactc
    17341 ttttctcagc ttttacatct aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta
    17401 gaaacaccag aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg
    17461 tttctttcac gcccggcacc gatagaaacg ctgaatacgg cggttctggc gctataagct
    17521 gcctcatttt ccgcctggct ggactatctt taggactggc acaggaagaa tatgaggaag
    17581 cgaacgatga gtaaggcagg caaaataacc gctgcgattt caggggcttt cttgttgttg
    17641 attgtcgtgg cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc
    17701 aaccagaaag tctctgcgga gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg
    17761 gtgtgggagc ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gccccatgta
    17821 cacgcggaag acatcattct tggcaatcca ccggatattc ccgaagtcac aatggtgcat
    17881 ttgccgcgag tagaagcgac gctggccccg ctggcgctgc tgaccaaaac ggtctggttg
    17941 ccgtggatca agctcgaaaa gcccgacgcg cgcctgattc gcctctccga aaagaacaat
    18001 aactggacgt ttaatctcgc caacgatgac aacaaagacg cgaatgcaaa gccgtcggcg
    18061 tggtcgtttc agctggataa tattcttttc gatcaagggc ggatcgccat tgatgataaa
    18121 gtaagcaaag cggatctgga aatttttgtc gatccgttag gcaagccgct gccgttcagc
    18181 gaagttatcg gatcgaaagg taaagcggat aaagaaaagg tgggcgatta cgtttttggc
    18241 ctgaaggcgc agggacgtta taacggtgaa ccgctcactg gtacgggaaa aataggcggt
    18301 atgctggcgc tgcgtggcga aggaacgccg tttccggtac aggctgattt ccgctctggt
    18361 aacacccgtg ttgcttttga tggcgttgtg aatgacccaa tgaagatggg gggtgtcgat
    18421 ttacggctta aattttctgg cgattcgctg ggtgatctct atgaactgac gggcgttctg
    18481 ctgcccgata caccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa
    18541 aaatcgtcgg tctttgatta tcgcggtttt aatgggcgca ttggcgatag cgatatccac
    18601 ggttctctga tctataccac cggcaagcca cgaccaaaac tggaaggtga tgtcgagtcg
    18661 cggcaattgc ggttggcgga cctgggaccg ttgattggcg ttgattccgg gaaaggggca
    18721 gaaaagtcga aacggtctga acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa
    18781 gtgctgcctt atgaccgctt cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc
    18841 ttcaaagggc ggcgcattga gcatggcagc agcctgccga ttagcgatct ttctactcat
    18901 atcatcctca aaaatgctga cctgcgcctg caaccgctga aatttggcat agcgggcggc
    18961 agcattgcgg cgaatattca tctggaaggc gataaaaagc cgatgcaggg gcgggcagat
    19021 attcaggctc gtcgactgaa actgaaagaa ctgatgcccg atgtagaact gatgcagaag
    19081 acgctggggg aaatgaacgg tgacgcggaa ctgcgcggta gcggtaactc ggtggcggcg
    19141 cttttaggca acagtaacgg caacctgaaa ctgttgatga atgacgggct ggtgagccgc
    19201 aacctgatgg agattgttgg gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt
    19261 gatgatgagg tgcgggtgaa ctgcgcggca gcgaatctgg atattgccaa cggtgtggcg
    19321 cgtccgcagg ttttcgcttt tgataccgag aatgcgttga tcaatgttac cggcacggca
    19381 agttttgctt cggaacagct ggatttaact atcgatccag aaagtaaagg gattcgcatt
    19441 atcacgctgc gttcgccgct gtatgtgcgt gggacgttta aaaatccgca ggctggggtg
    19501 aaagccgggc cgttgattgc ccgtggtgct gttgcggcgg cactggcaac gctggttacg
    19561 ccagcggcag cattgctggc actgatctca ccttccgaag gggaggctaa tcagtgccgg
    19621 acaattttgt cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt
    19681 aaacagattt agattgttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc
    19741 tccggcttac aaatccttgc taattcaata tattgtatgg aaattctggc ctgataagcg
    19801 tagcgcatca ggcttttcac tctgattaca acgactgatg tcgcgtctca tgggtcagca
    19861 gcagggcgat taacgtcaag ccagccatcg ccgccagata tagccccacc gcacctaacc
    19921 cgtagttagt ctgcaaccag gctgcgatat atggcgcaac ggaagccccg agaatcgacg
    19981 atacgttgta agagaacgat gctccggtgt aacgcacttc tgtcgggaac agctccggca
    20041 gcagcgcccc catcggaccg aaggtcagcc ccatcagact taaccccagc agcaggaagg
    20101 caaaaaccag aatcgggttg ccagaaccga gcaaagggtt aaaggcgaat agcgcgaaca
    20161 ggatgatcag cgtggtgatg attaccatgc ttttacggcg accaaaggca tcagccagta
    20221 atccggcgac gggcaccatc acgccaaaac caataactgc catcatcagc atccacaaca
    20281 cttcgttacg cggcaggcca agcccaactg gcgcggcggc ggtactaaag gtcatagagt
    20341 agaccgtcat gatgtaaaac agcgtatagg ttgccagcat aatgaacgta cccagtacgg
    20401 ttacgcgaac atgtttggtc agcagcgtac ccagcgggat cttcacctgt tttttcgctt
    20461 tagcgacttt ctcaaacacc ggcgactcat gcagcgacac gcgaacatac aggccgataa
    20521 tgaccagcac cgccgagaag ataaatggca cacgccagcc ccagctcata aactgctcgt
    20581 cggtcagtag ccaggaaagc agcaagaaag tgccgttggc aaagaagaag ccaatcggtg
    20641 cgcccagctg cgggaaggag ccgtacaatg cacgtttgcg cggtggggcg ttttcagtcg
    20701 ccagcagcgc cgcgccgccc cattcaccac ctaagcccag accctgacca aatcgagcca
    20761 gcgccagcag cagcggggcg aaaataccaa tcgtggcata gcctggcagc agaccaatca
    20821 ccacggtcga aatccccatc gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc
    20881 gatcgccaaa atgaccaaaa acggcagagc caatggggcg cgcgacgaag gcgatggcga
    20941 aggtggcgag cgactgtagc gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg
    21001 gaaacacaat aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc
    21061 caatgagaga ggcgacaagg actttattac gcgagttgat cggcgtgtat tcttgctcgt
    21121 ggtcgagtgt tgtggctgtt gcttgcataa tcttttctta ttttttggct aacgaatagc
    21181 catattacgc acagcaaaag tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa
    21241 atcatgcaaa aaggggataa tgtttaggcc agtgattttt cttctatgaa atgcttcaca
    21301 taattttata aatggtggat taaactggtt ttaggttaaa taaaagttac caactggatt
    21361 tatatgctgg ggttatgagt tggaatgaaa ttgtttgctc ctgaatagat ggctaaagag
    21421 gctctgggaa agagtttcaa gaggttatgt gcgcattata gccggatgcg atgctggcgc
    21481 atcttatccg gcctacgggg agccaggccg aataaattcg tacgccgcat ccggcctatt
    21541 ttaaggctgc gttttccccg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca
    21601 cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac
    21661 ggaaccaaat gctgcgccag agggtgattt catcaacgat ggcagggtgt aggtcatcac
    21721 gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcggatca gggctttttt
    21781 gcgcggacgg tggcgcggca accgccagaa gatccagaag aaaagcagat agttggcgaa
    21841 gatggaaatc gccaggccaa tcaaccgcca tgtcggttta agccactcaa tgctgttcag
    21901 gtgcagggcg ctaataatca tttgctgcgc agaaccggca accgaggtga tagaaagcgt
    21961 cacaatcagc gcaatcaaca aaccaatcag cgaaataaaa tcacgcagat attttaccca
    22021 gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgtgactggg cgcgaatcgc
    22081 ttcacgcagg ttacccatcc agttgatgcc ggaataaagc gccactgcca gaccgacaag
    22141 ccctacagtc gtccgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag
    22201 cgtcggatcg ctaatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg
    22261 ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatgggga tcatcgacaa
    22321 aaatgagaaa taggtgatag ccgccccaaa ctggttaccc agccgatcgt taaagcgttc
    22381 tgtcgcgcga atcaggtgcg caataaccgg ttggcgttgg actttctcgg cagtggtggt
    22441 gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat
    22501 cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc
    22561 ttttgtcctt ctgtcgtggt tattcttaaa aagtatagtc ggtcagtcca ggtgctcttt
    22621 cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg
    22681 cggataaacc agtgaaaggg acagcggctc ggcacggtag ccaggcaaca cttcaataag
    22741 ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc
    22801 ggcaataccg gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt
    22861 aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg
    22921 cggtgtataa cgcactattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa
    22981 gcgcgtcaga tagtgtggac tggcacagtt gaccatggtc agtttgccga gagaacgggc
    23041 gataacgcca tcttccggta atgcgccagt gcgtatcacg caatcaaaac catcatgaag
    23101 aatatctacc ggacggtcat ggctactcag ttccagctca attcccggat gcagatagag
    23161 aaattccgac aagcgcggca gtaacaggct tttcgcgatt cctgacggga tgtcgatgcg
    23221 taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt
    23281 actcaacaca tcttttgctc gttgataata ggtcatgcct tctggcgtga gtttgacccg
    23341 tcgcgtggtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg
    23401 cgaaacactt ccctttggca aagcaaagaa atccgctgcg cgggaaaaac tttccagctc
    23461 cgcgactttg atgaacaact gcattgcgtg aattttatcc atctctttga ccgattgttg
    23521 ttcacaacga aacagtagcg cgcatatcgc attgtttatt gttttttcta acatctaatg
    23581 ccgggtttca caatgttgtc atgatgaatg tggcgatttc tcagcaggaa aagggaggaa
    23641 tagctttatg agaaaggtga attcccttaa acagagcgta tttttcagcg attcccttta
    23701 aagtgaatag aagatgtatt ggctatttgt gcttattttt atctcattgc cgtttgttat
    23761 ttttgtttat ggtcgcggaa tttcattttt gattgcatgc gtactaacta ccgatataag
    23821 gaaatcctca aaaatgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt
    23881 aactatttat atcggtaact attttctacg cgttatctct gtagaatggc caccgccaaa
    23941 tggcgaattc ttattttttc tcgcgagtaa tacccttcca ctacttaagt ctttttaacg
    24001 tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt
    24061 catggaatga aaatcagttt acagcatcgt attccaggag tgagtattca gggggccagt
    24121 caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt gatgctcgat
    24181 ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcaatttcct
    24241 gaggttaagg tgttaattac ggcgacggat tgcaacaaac ggtggttaca ggaagttatt
    24301 cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg
    24361 gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcgaac gacgccggaa
    24421 aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta
    24481 gcggcgggcg aatcaaataa agagatcggc agggcgctga atatcagtac cggaacggta
    24541 aaagcgcatc tggaatcact gtatcgccgt ctggaagtca aaaatcgcac ccaggcggca
    24601 atgatgttaa atatctcctc ctgaagagga taattttaca gccagcagga agttagcggc
    24661 tggctgtaaa aatattatgg ttcgccgtac aaaccaatta aacggcgtac cacaccgtta
    24721 gtccagccaa acccatcctg caacggatac tcgccaccgc cgccttcgcg aggaacacca
    24781 tcggcaatat ggtatttctc gatcagtttg tgctgttcca gatagaactg attcaccgtc
    24841 ttcagccagt tgcgcgcgat ttcatcaccc agaaggtcat cgccgtacat cttaaatccc
    24901 tgaattgcca tccactgtaa cggtgcccag ccgttgggtt tatcccactg ttcaccggtt
    24961 tcgtactcgc ttgccagaat cccgccaggt gtcagtaacc ggctgcgcac ggcgtttgcc
    25021 agacgatcgg cctgttcatg gttcgccata ccgacataga gcggcacaat ggcggcagcg
    25081 gaaaacagcg ccagttgctc gcgtcgccag tcgtaatcgc ggtagatgcc gttttcatca
    25141 tcccagaggt aacggtttac cgcatcgcga cgggcactgg ccttctggcg gaacagtgct
    25201 tctgtctctt tctcgtcttt cagcgccgag atgttggcga tggcgctctc cagtttaaac
    25261 aggaaggcat tcagatcgat ggggatgaac tgggtggtac gaatgctcgc cagacgacca
    25321 gtatcacgca gccaacggga agagtaatcc caaccggagg ccgcccccgc acgtaaatcg
    25381 cggtacacct cgttaggcgg gcgaccagaa tgtttcgcgg tttcaacgtc ctcaagccag
    25441 gattcgtcac gcggcgtgtc gcgatcgtcc cagtaacggt tgagcagcga gccgtccggc
    25501 atccgcacaa catggcgata ggcctgatta gggatcaacg attctgcacc gtccatccag
    25561 aaggcatatt ccattttcag gtggtcgaga tagcggcgcg cgccgcgtac accatcttct
    25621 tcaaacaact ccaccatcag cgcaaaaacc ggtggttgcg agcggctcaa ataataggtg
    25681 cggttgccgt tggggatgtg gccgtagttt tcgatcatcc aggcgaagtt atcagccatg
    25741 catttcagca aatcttcccg accactttcc gccagcccca gcatggtgaa ataggaatcc
    25801 caatagtagg tttcgctaaa acggccgccc gggacaatat atgactgcgg cagcgccagc
    25861 agagaagacc acggaatgtg atcctgtggt tcatgcgtta gcaccggcca cagctggtcg
    25921 atatgctctt tcagggaatt ttgcgggtcc gatacatact cgctggagta gacctccggc
    25981 agccagaagt ggttttcaac aaacttgcgc aagtcaaaat cacgatgacg gcgcacttta
    26041 cggtagcgga ttaaaatatc cagcgggtcc attttgggtg cgcagtcggg aaaggtttta
    26101 ctgtcgggga aaattttcgc cgactgaaca tgctcgaaca gttcgagata gcgatcggca
    26161 ggcgtcagcg catcagaggc gggcagccct tcaatcatct cgggttccgg ttctgcctcg
    26221 atcatctcat ccagttttaa ttcatacggg tccagctcat agcagagatc gacctcgatc
    26281 atcagttcgt ctggattagg gttttgaatt ttctgattga gcataaggat ccgacctccg
    26341 aaattcgtcg ctaattatcg ggcttttctt taagggtagt cattcgctcc gcctggtggg
    26401 cgacaaagcg tgcggtggat cacgtttatt aacctgccaa aaaatataac gcactaatat
    26461 caatggaatt tataaaaata cggatttcgt gttgtttttc attttcagaa cattcaatcc
    26521 tgctctcgcc gcaatcatga tatgccattc ctgatctggg taata
//
LOCUS       DAZFRG010000042        26332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFRG010000042 DAZFRG010000000
VERSION     DAZFRG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.042"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..345)
                     /gene="minE"
                     /locus_tag="ACHKUC_001380"
     CDS             complement(79..345)
                     /gene="minE"
                     /locus_tag="ACHKUC_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO6617664.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(349..1161)
                     /gene="minD"
                     /locus_tag="ACHKUC_001381"
     CDS             complement(349..1161)
                     /gene="minD"
                     /locus_tag="ACHKUC_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO6617665.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1185..1880)
                     /gene="minC"
                     /locus_tag="ACHKUC_001382"
     CDS             complement(1185..1880)
                     /gene="minC"
                     /locus_tag="ACHKUC_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO6617666.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEAPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2400..2768
                     /gene="ycgJ"
                     /locus_tag="ACHKUC_001383"
     CDS             2400..2768
                     /gene="ycgJ"
                     /locus_tag="ACHKUC_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO6617667.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVN
                     GKRNGKINQTTTKMLFMCRE"
     gene            complement(2871..3272)
                     /gene="pliG"
                     /locus_tag="ACHKUC_001384"
     CDS             complement(2871..3272)
                     /gene="pliG"
                     /locus_tag="ACHKUC_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO6617668.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3514..3807
                     /locus_tag="ACHKUC_001385"
     CDS             3514..3807
                     /locus_tag="ACHKUC_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO6617669.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            3879..4538
                     /gene="ycgM"
                     /locus_tag="ACHKUC_001386"
     CDS             3879..4538
                     /gene="ycgM"
                     /locus_tag="ACHKUC_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6617670.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            4630..5076
                     /gene="ycgN"
                     /locus_tag="ACHKUC_001387"
     CDS             4630..5076
                     /gene="ycgN"
                     /locus_tag="ACHKUC_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO6617671.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5283..5606)
                     /gene="hlyE"
                     /locus_tag="ACHKUC_001388"
                     /pseudo
     CDS             complement(5283..5606)
                     /gene="hlyE"
                     /locus_tag="ACHKUC_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            5869..6288
                     /gene="umuD"
                     /locus_tag="ACHKUC_001389"
     CDS             5869..6288
                     /gene="umuD"
                     /locus_tag="ACHKUC_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6617672.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            6288..7556
                     /gene="umuC"
                     /locus_tag="ACHKUC_001390"
     CDS             6288..7556
                     /gene="umuC"
                     /locus_tag="ACHKUC_001390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO6617673.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(7601..8131)
                     /gene="dsbB"
                     /locus_tag="ACHKUC_001391"
     CDS             complement(7601..8131)
                     /gene="dsbB"
                     /locus_tag="ACHKUC_001391"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO6617674.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(8277..9818)
                     /gene="nhaB"
                     /locus_tag="ACHKUC_001392"
     CDS             complement(8277..9818)
                     /gene="nhaB"
                     /locus_tag="ACHKUC_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO6617675.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10040..10759
                     /gene="fadR"
                     /locus_tag="ACHKUC_001393"
     CDS             10040..10759
                     /gene="fadR"
                     /locus_tag="ACHKUC_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO6617676.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(10922..12454)
                     /gene="ycgB"
                     /locus_tag="ACHKUC_001394"
     CDS             complement(10922..12454)
                     /gene="ycgB"
                     /locus_tag="ACHKUC_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO6617677.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            12784..14082
                     /gene="dadA"
                     /locus_tag="ACHKUC_001395"
     CDS             12784..14082
                     /gene="dadA"
                     /locus_tag="ACHKUC_001395"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO6617678.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14092..15162
                     /gene="dadX"
                     /locus_tag="ACHKUC_001396"
     CDS             14092..15162
                     /gene="dadX"
                     /locus_tag="ACHKUC_001396"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO6617679.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPRARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15219..16955)
                     /gene="cvrA"
                     /locus_tag="ACHKUC_001397"
     CDS             complement(15219..16955)
                     /gene="cvrA"
                     /locus_tag="ACHKUC_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO6617680.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PTGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17050..17964)
                     /gene="ldcA"
                     /locus_tag="ACHKUC_001398"
     CDS             complement(17050..17964)
                     /gene="ldcA"
                     /locus_tag="ACHKUC_001398"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO6617681.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18064..18675
                     /gene="emtA"
                     /locus_tag="ACHKUC_001399"
     CDS             18064..18675
                     /gene="emtA"
                     /locus_tag="ACHKUC_001399"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO6617682.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18677..19411)
                     /gene="ycgR"
                     /locus_tag="ACHKUC_001400"
     CDS             complement(18677..19411)
                     /gene="ycgR"
                     /locus_tag="ACHKUC_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO6617683.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLLLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            19612..19866
                     /gene="ymgE"
                     /locus_tag="ACHKUC_001401"
     CDS             19612..19866
                     /gene="ymgE"
                     /locus_tag="ACHKUC_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6617684.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19916..21886)
                     /locus_tag="ACHKUC_001402"
     CDS             complement(19916..21886)
                     /locus_tag="ACHKUC_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6617685.1"
                     /translation="MRLKKRYLCTVLSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYHDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(21912..22766)
                     /gene="modD"
                     /locus_tag="ACHKUC_001403"
     CDS             complement(21912..22766)
                     /gene="modD"
                     /locus_tag="ACHKUC_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO6617686.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(22763..23575)
                     /locus_tag="ACHKUC_001404"
     CDS             complement(22763..23575)
                     /locus_tag="ACHKUC_001404"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6617687.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            complement(23585..24343)
                     /locus_tag="ACHKUC_001405"
     CDS             complement(23585..24343)
                     /locus_tag="ACHKUC_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6617688.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGVNGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(24340..25320)
                     /locus_tag="ACHKUC_001406"
     CDS             complement(24340..25320)
                     /locus_tag="ACHKUC_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6617689.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(25320..>26332)
                     /locus_tag="ACHKUC_001407"
     CDS             complement(25320..>26332)
                     /locus_tag="ACHKUC_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO6617690.1"
                     /translation="RRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITRIVSA
                     GAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSLEGLM
                     ALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLGEEHR
                     AAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLGLHNV
                     AQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRILFLSG
                     LPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDIQHYAQLFWHCSLSDADYQKLV
                     AS"
BASE COUNT         6556 a         6512 c         6803 g         6461 t
ORIGIN      
        1 tttatcaata ttattgtgca cagaaactca aggcagagat gactctgcct tgaagataaa
       61 tgcgctttta cagggggctt atttcagctc ttctgcttcc ggtaaggtca cgttcagctc
      121 aagaatagaa atatcgccat ctttttgctc aagctgtacg gttaccatct caggatcaat
      181 ttgtacatat ttacaaatga cctcaagaat atctttacgc aactgcggca gataatgcgg
      241 ttctgcatcg ctgcgacggc gttcagcaac aataatctgc aaccgttctt ttgcaatgtt
      301 ggctgtgttt ttcttccgcg agagaaagaa atcgagtaat gccataactt atcctccgaa
      361 caagcgtttg aggaagcctt tcttctcttc ttcaatgaag cggaaaggac gttcttctcc
      421 caacagacgt tctacggtat ctgcgtaggc tttacccgca tcggcgttaa tgtcgagaat
      481 gaccggttca ccctggttag aggcgcgcaa tactgattga tcctctggga tcacgccgac
      541 gagtttgatg cgcaggatct ccagcacatc ttccatgctc agcatgtcac ctctgcttac
      601 gcggcctggg ttatagcgcg ttaacagtag gtgctcttta atcggctctt cgccattttc
      661 tgcgcggcgt gatttcgacg ccagaatgcc taaaatacgg tcagagtcgc gtactgagga
      721 gacttccggg ttggtggtaa taatggcttc gtctgcaaaa tagagtgcca ttaacgcacc
      781 ggtttcaatc cctgccgggg agtcacaaac gataaattca aaatccatcg ctttcagatc
      841 atcaagaact ttggcgaccc cttcacgggt gagggcatct ttatcgcgag tttgcgatgc
      901 cggaagaata tagagatttt cagtacgttt atctttaatt aacgcctggt ttagcgttgc
      961 atcgccctga atgacgttga cgaaatcgta aacgacccgg cgttcacaac ccataatcag
     1021 gtcgagatta cgcaggccga tatcaaaatc tatcacgaca gttttctttc ccttctgggc
     1081 caaaccagtg gcgatggccg cgctggaggt tgtcttacca acaccccctt tgcccgaagt
     1141 aacaacaata atgcgtgcca tagaaattcc ttgttaaaaa gggatcaatt taacggttga
     1201 acggtcaaag cgttttcgac taactgcagt cgtgccgctt tgccataaaa ttctgctggg
     1261 atttgatcac tcagccagta ttcacctgcg atggacacca gttccgccat caggttcgta
     1321 caaaatattt gcgtttcccg gtcaccactt gcccctgcca gcgcacgacc gcgcatcatg
     1381 ccatagacat gaatgttacc atcggcaatc aattcggccc ctgcgctaac gtggcttgta
     1441 acaatcagat cacattgtgg agcataaata cgctgaccgg aacgcactgg ggtatcaatt
     1501 aaacgcgttt ttgtgaccgg cgttgtattt tgcgctggag cctgcggtgc gggagctgga
     1561 cgcggcgctt tttcctttcc ttccgtcagg ataggcagcc ccatcttttc aatttcggct
     1621 ttaagttgcg catctttgca gccacttacg ccaataaccc gcaaaccggt tgccgaaacc
     1681 gccttatgca tcgctgacca gtttaccggg gcttccagtg cacttacgtt gagtacaacg
     1741 ggggcatgtt ttaaaaatgc gggggcctga gcgattttgt cttccagcgc ctgatggata
     1801 accttaggtt ctgcctcatg cagatgaacc acagataaag tgaagctact gcctttaagc
     1861 tcgattggcg tgtttgacat cctggcctta ctcaattagc tattaatcat cgccagcgcg
     1921 cgatgatgtt ccgaagacta taaggcatgt tatagtctgg attatattga ggcaagtcac
     1981 cctcccattt attcagagta aaagtctatt ctgtgataaa tggcgctgat tcatagctta
     2041 aaaaataccc ttgtcaatca acccattgcc gtcgtacttt tgattgttct tatttacgct
     2101 tcctttttcc gcaccctaat cgtgacagca tcttcaggcc tgtttaaata tccagtgttg
     2161 aggcgagtgt attttttcac cacgccaggc tttcttaact ttccattttt tgcagcatca
     2221 ttaaagaaca gatacccgtt atcttaatat ccgccacaga attgctctat catagaaacc
     2281 gcagaagacg tctgatactt ctgtcaggtc atcgtaactc gtccaggtca gcggttttga
     2341 ttacggattg atgatgaaat gagcataagt gttgtacggt tggattatca ggatgtgcta
     2401 tgaatatgat gcgtattttt tatatcggat tgtcaggtgt ggggatgatg ttctcatcaa
     2461 tggcgtctgg aaatgacgct ggcggacttc aatctccggc gtgcggcgtt gtttgcgacc
     2521 cgtatatttg tgttaactca gatggcattt ccccagagtt aacaaggaaa tatctcggcg
     2581 aaaaagccgc tgaaaactta caatcagtac aaggttacga tcccagcgaa tttacattct
     2641 ctaacggtgt attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg
     2701 tgaatggtaa gcggaatgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc
     2761 gtgagtgatg tcttgtttac attatgttct attcgtcatt aacctcttaa gcgacagaga
     2821 ggagtggggc gttaacgttt atgacgggga gagtccccgt cggttgatat ttacttaatt
     2881 tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc
     2941 actcgcagct catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct
     3001 ggggaatatc tggataaatc aacagaatca tcgattcctg gcccgaagag gtaagtatca
     3061 gcgccttcgt tagagatact cacgtgcact ttctgacctt ttttggcata gaaggtatat
     3121 gtatcgtaat cgtatccctt tatttcgccg gagtactgag cgctgctatg gccttttcta
     3181 aactcaacat tgacattctt acccgcagca aagctcgtgg aggttagcag tgcaagcaac
     3241 aatacagcct tactgatgcc cttaattttc attggtagtc tcctgttcag aatgagtacg
     3301 atgaactgtt ataatataac aatccttcac gggagaattc cagtgacttt cctgtgttcg
     3361 gttaaatccc ggcgcggcat ttttgccggg gggattactt ataaaggggg catggactga
     3421 acaaaccgta gaaatctaca gctggctggc ttgtggcgcg ggtttcattg atgttagctt
     3481 atgccaaaac ctggcatctt aaagagtaaa agtatgtttt gtgtgattta tcgaagcagc
     3541 aagcgtgacc agacctattt atatgtcgaa aaaaaagacg atttttcgcg tgttcctgag
     3601 gaactgatga aaggttttgg tcagcctcag ttagcgatga ttcttccgct ggatgggcgg
     3661 aagaaactgg ttaatgccga tattgaaaag gttaaacagg cattaaccga gcaaggttac
     3721 tatttgcagt taccgccacc acccgaagat ttgctgaagc aacatctttc cgtcatgggg
     3781 caaaaaacag acgacattaa caaataaccg atatccggcg gtggcattat ctttgtcggc
     3841 gcgggttttc atatccacga taaggtgagg ggaacgttat gtatcaacat cacaactggc
     3901 aaggtgcgct gctggattat ccggtgagta aagtagtctg tgttggtagt aactatgcca
     3961 aacatattaa agagatgggc agcgcagtgc ccgaagagcc agtgctgttt attaaaccag
     4021 aaacggcact gtgcgatctg cggcagccat tggcgatccc atccgatttc ggttcagttc
     4081 atcatgaagt cgaactggcg gtgttgattg gcgcgacgct gcgtcaggct acggaagagc
     4141 atgtccgcaa agccattgcc ggttacggcg tggcgctcga tctgacattg cgtgatgttc
     4201 agggaaaaat gaagaaagcc gggcagccgt gggaaaaggc caaagcgttt gataactctt
     4261 gtccgctttc cgggtttatt cccgcggcgg agtttaccgg cgacccacaa aatacaacgc
     4321 tgggcctgag cgtaaacggc gagcaacgcc agcaaggtac gactgcggac atgatccata
     4381 aaatcgttcc gctgatcgct tatatgagca agttttttac cctcaaggcc ggtgacgttg
     4441 tgctgacagg cacgcctgat ggtgtcggcc cgttgcaaag cggtgatgag ctgacagtca
     4501 ctttcgatgg gcattctttg acaactcgcg ttttgtaata ctttttgccg cctgaaagcg
     4561 gcggcaaaac ttgcatcgct gtgccagact ggttataagg tgcgttttaa cgtaatggcg
     4621 gaacacctga tgagcgatgt acctttctgg caaagtaaaa ccctggacga aatgagcgat
     4681 gcggaatggg agtcgttgtg tgatggttgc ggtcagtgtt gcctgcataa actgatggat
     4741 gaagacaccg acgaaatcta cttcactaac gtcgcctgtc gccagctcaa tattaaaacc
     4801 tgtcaatgtc ggaactacga acgtcgtttc gagtttgaac ccgactgcat taaattaacc
     4861 cgtgaaaatc tgccaacatt cgaatggctg ccaatgacct gcgcttatcg tttgctggcg
     4921 gaaggtaaag gtttacctgc gtggcatccg ctacttactg gttcgaaagc ggcaatgcat
     4981 ggtgaacgta tctctgtgcg tcatatcgca gtaaaagaat cagaagtgat tgactggcag
     5041 gatcatatct taaataaacc tgactgggcg cagtgataaa tttaatagat gttgatgggg
     5101 gctttcttaa tatacgtctg gatgctcgcc atttcgtagc cctgtttttg aaagcgctct
     5161 gtcataatga gagttactcg ataccttatt atcaacaggt aactctcaat ctttaaaaaa
     5221 cgtctgtatt tttaaatgtg ataaacttta taccttgagt acatagagag aataatcgct
     5281 tatcagactt caggtatctc aaagagtgtc ttcttaccgt gtcttttctg atactcatta
     5341 caagtgttaa ttattttttt ggccgcttct tttagtaaag aaagcattaa atcatcataa
     5401 tcaacgtaga atctggttgt ttcagtttcc gtttttatct ccccgatggc ggctatttcg
     5461 gtggttaatt tcaatttggc ggcatcgata tctttattcg cttgtttaac cgtgttagac
     5521 agggtggtaa agaaactctg cacagatttt aacttgttct tcaattctgg aatcagtttc
     5581 ccttcaacta cgcccgcagc aatagaacac agtttcttga tgccgtcatc cagtatctta
     5641 atgagaatgc ctttctgggc gaatgcttcg attctatttt aaaccaggtg atatttttcc
     5701 tttctgctgg atggacgctg tcgccagaag gccgcgtgag cacaggataa gagaacgaaa
     5761 aatcagcagt ctatgcagcg acaaatattg atagcctgaa tcagtattga tctgctgaca
     5821 agaacagact actgtatata aaaacagtat aacttcaggc agattattat gttgtttatc
     5881 aagcctgcgg atctccgcga aattgtgact tttccgctat ttagcgatct tgttcagtgt
     5941 ggctttcctt caccggccgc agattacgtt gaacagcgta tcgatctgaa tcaactgttg
     6001 atccagcatc ccagcgcgac ttatttcgtc aaagcaagtg gtgattctat gattgatggt
     6061 gggattagtg acggtgattt actgattgtc gatagcgcta ttaccgccag ccatggtgat
     6121 attatcatcg ctgctgttga cggcgagttt acggtgaaaa aattgcaact acgcccgacg
     6181 gtacagctta ttcctatgaa cagtgcgtac tcgcccatta ccatcagtag cgaagatacg
     6241 ctggatgtct ttggtgtggt gatccacgtc gttaaggcga tgcgctgatg tttgccctct
     6301 gtgatgtaaa cgcgttttat gccagctgtg agacggtgtt tcgccctgat ttatggggta
     6361 aaccggtggt tgtgctatcg aataatgacg gttgcgttat cgcccgaaac gctgaggcaa
     6421 aggcgctggg cgttaaaatg ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct
     6481 gtggcgtggt ttgctttagc agcaattatg agctttacgc agacatgagt aatcgggtga
     6541 tgtcgacgct ggaagaacta tcgccccgcg tcgagattta cagtattgat gaggcattct
     6601 gcgatctgac aggtgtgcat aattgtcgcg atctgactga ttttggcaga gaaattcgcg
     6661 caacggtgct acaacgtacc catcttactg ttggtgtggg gatcgcccag accaaaacgc
     6721 tggctaagct tgccaatcat gcggcaaaaa aatggcagcg gcagacgggc ggggtggtgg
     6781 atttatcaaa tctggaacgc cagcgtaaat taatgtctgc tctccccgtg gatgaagtct
     6841 gggggattgg acggcggatc agcaaaaaac tggacgcgat ggggatcaaa accgttctcg
     6901 atttggcgga ttcagatatc cggtttatcc gtaaacattt taatgtcgtg ctcgaaagaa
     6961 cggtgcgtga actgcgcggt gaaccctgtt tgcaactgga agagtttgca ccgacgaagc
     7021 aggaaattat ctgttcccgc tcgtttggtg aacgcatcac ggattatccg tcgatgcggc
     7081 aggccatttg tagttacgct gcccgggcgg cggaaaaact tcgcagcgag catcaatatt
     7141 gtcggtttat ctccacgttt attaagacgt caccatttgc gttcaatgaa ccttattacg
     7201 gcaatagcgc atcggtaaaa ctgctgacgc ccactcagga cagcagggat atcattaacg
     7261 ccgctacgcg atctctggat gccatctggc aagcgggcca tcgctaccaa aaagcgggcg
     7321 tgatgctggg ggatttcttc agtcagggag tcgcgcagct caatttattc gatgacaacg
     7381 caccgcgccc cgggagtgag caattgatgg cggtaatgga tacgctgaat gctaaagagg
     7441 gcagaggaac actctatttt gccgggcagg ggatccagca acaatggcag atgaagcgtg
     7501 ccatgctttc accatgttat acaacgcgaa gttctgattt actgcgggtc aaataaacat
     7561 agcggcagga aaaagcgctc ccgcaggagc gccgaatgga ttagcgaccg aacaggtcac
     7621 gttttttcgc tttaaacggc tgggaaatca ccaccagcac cgcgacaatc aggtaagcga
     7681 taaaaatacc gagcagccac tgcggcattt ccagacctaa aaattcccac tgacgctcgg
     7741 cgcaatcgcc agaggcgaca aacacttgcg gcacccattt atccagcggc agccattccg
     7801 ggaaacgagc cataaaatca caggtcgcaa acggcgaagg atagagctga agcatggtgt
     7861 gctcgtaagt taactgcaca ccgcggaacg cactatacaa ccagataacc atcgctacat
     7921 aacgcagcgg agttttcggg gcgatcgcgc caatcagcgc agcacccaga acgccgaata
     7981 acgcgcagcg ttcataaata cagagcacgc aaggtttcag taacatcaca tgctggaacc
     8041 acagcgccgt cagttccagt gccagagcag taaacgccat caacagccat gcgccccggc
     8101 cttgtgaaca ttggttcaaa aatcgcaaca taatcatttc cctgcaatat gcatagagtg
     8161 cgcagtttaa accaattcat tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt
     8221 ggacaattat cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa
     8281 agcgttgcta tccagcccat ttgcataaac cattcggtta caggggcaag cgtaaactcg
     8341 acgcagagca agccgacgag tgtcaggacg agggtgtaag gcagggccat ccacaccatg
     8401 cgcccataag agaggcgaat caatggcgcg agtgcagagg tcagcaggaa caggaacgca
     8461 gcctgaccgt tcggcgtagc gacggagggc agattggtac cggtattaat ggcgaccgcc
     8521 agcagctcgt attgcttcaa cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg
     8581 atataaatcg tccccacgaa gacgttatcc gaaatggatg acagcagacc gttgaaaata
     8641 tagaacagcg acagctgagc atgttccgat gcctgcaaca caaactgaat aatcggcgaa
     8701 aacagttgtt ggtcgataat caccgcgact accgagaaaa acaccgtcaa cagtgcggtg
     8761 aatggcagag attcggtgaa ggctttaccg atagcatgtt catcggtgac accagtcaat
     8821 gatgtcgcca gaataatgac tgacaaacca atcaagccaa cttccgccag atgcaacgcc
     8881 agcgcagtca ccagccagac gccaataatc gcctggacaa tcagacggat tttatcctga
     8941 cgggtacgct ggtggcggct ttgatcgtca aactgttgca acacttcgcg gactttctcc
     9001 ggcagcgttt caccgtagcc aaaccaacgc agcttctcta ccagcaggca ggttaacagg
     9061 ccacaaatca gaaccgggac ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc
     9121 cagccagccg ctttagcgat gatcaggttc tgtggttcgc ccaccatggt catcacgccg
     9181 cctaatgcgg tgccaacacc ggcatgcatc atcaggctac gcagaaagcc acggaactgt
     9241 tccagaacca ctttgtaatg cttgtcgata tgactatcgt cttgcaggtc ggtatcttca
     9301 gtacgggaag aggctacgcg gtgataaata ccataaaaac cgacggcaac gctgatcacc
     9361 accgccacga cggttaaggc atcgaggaac gcggagagga acgccgccgc cacgcaaaaa
     9421 gagagcgaca gcagcatttt ggagcgaatg cttaacagca aacgggtaaa tatgaacagc
     9481 aatagctgtt tcataaaata gatacccgcc accataaaca tcagtaacag caagacttca
     9541 agatttgccg ccacctcttc acggacgtgt tccgcgctgg tcatgccgat gaataccgct
     9601 tcgatagcca acagaccacc ggggagcagc gggtagcatt tcagggccat cgccagagtg
     9661 aaaataaatt ccgctaccag caaccagccc gcgacgaaag ggctgatgag gaaaattaac
     9721 ggatttacga ttaagaaaat gatgagggcg agtttgtacc agtcggggga ctggcccaaa
     9781 aaattgcgcc atagcgcgcg gccccaggag atctccatga tggtttccct taccttacaa
     9841 ataatcaatg atgtttttat gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca
     9901 agtcttgata gtcaagggga aagagatgcg gaaaatgaag ccttgatccc ttttttcttc
     9961 tttttgtctg ctatcagcgt agttagccct ctggtatgat gagtccaact ttgttttgct
    10021 gtgttatgga aatctcacta tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga
    10081 gtacattatt gaaagtatct ggaataaccg cttccctccc gggactattt tgcccgcaga
    10141 acgtgaactt tcagaattaa ttggcgtaac gcgtactacg ttacgtgaag tgttacaacg
    10201 tctggcacga gatggctggt tgaccattca acacggcaag ccgacgaagg tgaataattt
    10261 ctgggaaacc tctggtttaa atatccttga aacactggcg cgactggacc acgaaagtgt
    10321 gccgcagctt attgataatt tgctgtcggt gcgtaccaat atttcaacta tttttattcg
    10381 caccgcgttt cgtcaacatc ccgataaagc gcaggaagtg ctggcaaccg ctaatgaagt
    10441 ggccgatcac gccgacgcct ttgccgagct ggattacaac attttccgcg gcctggcgtt
    10501 tgcttccggc aacccgattt acggtctgat ccttaacggg atgaaagggc tgtatacgcg
    10561 cattggtcgt cactatttcg ccaatccgga agcgcgcagt ctggcgctgg gcttctatca
    10621 caaactgtcg gcgttgtgca gtgaaggcgc acacgatcag gtatacgaaa cagtgcgtcg
    10681 ctatgggcat gagagtggcg agatttggca ccggatgcag aaaaatctgc cgggtgattt
    10741 agccattcag ggacgataat ccttccagac ggatgacaaa cgtataattg cctgatgcgc
    10801 tacgcttatc aggcctacgt gtttttttgc aatgtattga atttgcgata ctttgtaggc
    10861 cggataaggc atttacgccg catccggcaa aaatgagtcc tcctcaaacg aggaggacta
    10921 tttacagatt tcccattctc ggcgggcaac gttccagcaa ctcgacgctg ccgtcttcgt
    10981 tttgctgttc cagcatcaca tcaaatcccc acaggcgatg cacatgcttc agcacttctt
    11041 tgcgcccccg atccagcggt gcgcgattat gtggaatgta acgcagcgtc agcgaacggt
    11101 cgccgcgcaa atccacgttc cagatctgaa tattcggctc cagattactt aaattatatt
    11161 gagacgataa ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct
    11221 ccagataatt atgccgatcg tcatccagca cggtgaagaa gcggaaatca cgcatcactt
    11281 tcggtgacag gaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca
    11341 gcgtttccag ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg
    11401 gcgactgaca aatccgttta atatcctgga acatggcaaa cccgagggca tacgggttga
    11461 tgccgctgta ccacgggctg ttatagggcg gctggaagac cacattggtg tggctgtgca
    11521 aaaactccag cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg
    11581 tgtagtgcca gaaggtcgcc cagccttcgt tcatcacctg agtctgtttt tgcggataaa
    11641 aatactggct caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg
    11701 gcgcattttt ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg
    11761 cttcagcaac cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct
    11821 gactttgcag atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt
    11881 tttgcgggcg tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa
    11941 gccgttctac ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa
    12001 aaatcagata atcgacaatc gaactggcgt cggtccagct acggaataag taattgtttt
    12061 tgaagaaaga gttatgccca tagcaggcat gagccatcac cagcgcttgc atggtaatgg
    12121 tgttctcttc catcaggtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca
    12181 gtccttgctg accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc
    12241 agtgcggata gttaattggc atgccgacgc tggagtaggc atccatcatc tgttctgagg
    12301 ttatcacttc aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta
    12361 tctctgccag ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg
    12421 tggtgtcctt attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct
    12481 ctctgtgtgg agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag
    12541 aatttttttc tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat
    12601 tttatgctgg ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga
    12661 gtcagggaga tgtgagccag ctcaccataa aaaagctgta tgttgaataa tattttcaac
    12721 tgagttatca agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg
    12781 gctatgcgag ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta
    12841 aatcaggcag gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc
    12901 agtgccgcga atgccgggca aatctccccc ggatatgctg caccgtgggc ggcaccaggt
    12961 gtgcctttaa aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc
    13021 gacggtacgc agttccagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc
    13081 cactacatgg aaaacaaagg gcggatggta cgtctggcgg aatacagccg tgattgcctg
    13141 aaagcattgc gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa
    13201 ctgttccgta ctgaacaaca gtatgaaaac gcgacccgcg atatcgccgt gctggaagat
    13261 gctggcgtac cgtatcagct gctggaatcc agccgcctgg cggaagtgga gcccgcgctg
    13321 gcagaagtgg cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac
    13381 tgccagctgt ttacccagaa tctggcgcgg atggcggagc aggcgggggt taaattccgc
    13441 tttaatacgc ccgttgacca actgctttgc gacggcgagc aaatttacgg cgtgaagtgt
    13501 ggtgatgaag tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg
    13561 atgctcaaag gcattgttga tattccggtg tatccgctga aaggctactc gctgaccatt
    13621 ccaattgcgc aggaagatgg tgcgccggta tccactattc ttgatgaaac ctacaaaatc
    13681 gccattaccc gtttcgataa ccgcattcgt gtcggcggaa tggcggaaat tgttggcttt
    13741 aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaga tggtggttcg cgatctctat
    13801 ccacgcggcg gtcatgttga gcaggcgact ttctggactg gcctgcgccc gatgacgcca
    13861 gacggcacgc cggttgtcgg acgtacacgc tttaaaaatc tgtggctgaa taccggtcac
    13921 ggcacgctcg gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctcg
    13981 gggcgcacgc cagcgatccc gtatgaagat ctaagcgtag cgcgctacag ccgtggattt
    14041 acgccatcac gcccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt
    14101 ccgatacagg ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag
    14161 gccgcgccgc gcgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcacggtatt
    14221 gagcgtatct ggagcgcgct cggggctacc gatggctttg cattgcttaa cctggaagag
    14281 gcaataacgt tacgtgagcg cggctggaaa ggaccgatcc tgatgctgga aggatttttc
    14341 catgctcagg atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac
    14401 tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa
    14461 gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag
    14521 cagttgcggg caatggcgaa tgttggcgaa atgactctga tgtcgcattt tgccgaagcg
    14581 gaacatcctg atggaatttc cagcgcgatg gcgcgtattg agcaggcggc ggaagggctg
    14641 gagtgtcggc gttcgttgtc caattcggcg gcgactctgt ggcaccagga agcgcatttt
    14701 gactgggttc ggcctggtat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat
    14761 atcgccaata ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag
    14821 acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag
    14881 cgaatcggca ttgtcgccgc aggatacgcc gacggttatc cgcgccacgc gcctaccggt
    14941 acccctgttt tagtggacgg cgttcgcacc atgacggtgg ggacagtctc gatggatatg
    15001 ctggcggtcg atttaacgcc ttgcccgcaa gcgggtattg gtacgccagt tgagctgtgg
    15061 ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg gaacggtagg ctatgagttg
    15121 atgtgcgcgc tggcgttacg cgtcccggtt gtgacggtgt aacttgttat cgctggatgc
    15181 gaccagcgtc gtatccagcg ttacaaaaca cattaaactt aagattcggc ttcttcctcc
    15241 gctacccgaa caccaatctt caacacttca ttatcttctt tctcggccac cgtccagatc
    15301 atcccggcaa actctacctg gtcaccgaca accggtgctg cgcctaacaa ctgctggacg
    15361 atttcaccca gcgtttgctg cttatcgcga tattctcgcc cgtcttccag gccatatatc
    15421 agcgccacat cagcatattt ggcgctggct tcgagaatga agtcaccaaa gaagcgttga
    15481 tccagcgcga ccggcggcga ctggctgaac agtttaccga gcgccgggag atcgcgttcc
    15541 cgaccaatta cacacaacac atcgccttcg cgcagtcggg tgctgccggt gggatgaagc
    15601 aactggttat cacgaaacag tgccgcaata cgcgtctctt ttggcatatg caaatcacgc
    15661 agtgccgcgc ccacgcacca tttatcggca ctcaattgat aaacaaactg ctcccacgga
    15721 ttttccggat gaatatccag gccaacgcgt gacaccggac gtcccactgg cggaacgacc
    15781 actttggctt ttttcgccgc ccacgagagt gatgttccct gcaatagcag tgaaaccagg
    15841 accacaaaga aggcgacatt aaagaacagt cgcgcattct ccagccccgc catcatcggg
    15901 aacacagcca gaataatcgg caccgcgccg cgtaatccta cccagctgat aaacacgcgc
    15961 tcacgcagat tgaagccacg gaacggtagc aatccggcaa ataccgaaag aggacgggcg
    16021 aagaaaatca tccatgcgga caaaatgagc gccggaatgg caataggcag cagatcgctt
    16081 gggttaacca atagccccag caccaggaac atggcgattt gcgccagcca ggcgaggccg
    16141 tcgaaatttt gcaggatgcc gtagcggttg cgaatcggcc gattacccag cagaaaaccg
    16201 cacagataaa cagccagaat accgctgcct tccagcgccg tggttaaagc aaaaattaaa
    16261 ataccgccgc ttaatgccag caatggatat aatccggtgg gcagggcgat gcgattaatc
    16321 atttgcagca gtaaataacc accgccaagc ccgatgacaa tcccgaggcc aaattgctgc
    16381 agaatatcga ccacgaacat ccaactgaca ctgctttcat gttgctggat catcgcaatc
    16441 agggtaatcg tcagaaagac cgccattgga tcattactgc cggattcaat ttccagcgtc
    16501 gagccaacac gttcgttaag ccccttacca cccagcagag aaaagaccgc tgcagcatcg
    16561 gtggagccga cgatagcgcc gattaataat ccttcaatca aatcaagatt aaacagccac
    16621 gccgccatca tgccggttaa accagaggtg ataagcacgc ccagcgtcgc cagcgacagt
    16681 gccggtccta acgccacacg aaaggaactg gcctgagtac gcatcccgcc gtcgagcaaa
    16741 ataatcgcca gcgccaggtt acttaccatg taggcgaagg ggtaattatc aaacgggata
    16801 ccgccgacgc catcaacccc cgccagcatg ccgatcgcca gaaaaataac cagaatggga
    16861 atgccaagac gggaagaaaa tgaactaagt aatatactgc tggtgacaag gatggatcct
    16921 aagatgaaaa ggctaattat tgttgtggca tccaacggtc ggttactcct gattacgctg
    16981 tctcttatat aaaccctacc atattagcgg cagagacagc gttttactta gccctgaagc
    17041 gtgatttttt tacattttaa gaacaggatg accgctaatc gtcaactggg tgccttcctg
    17101 ggtgttatta agaatggcat gtgcgcccaa cggcagtgta accgtgcgtt gttcatgacc
    17161 aaaatcgagg ccggtaatga gtggaatcga caggcgggaa cgcaaaaacg cgtacactga
    17221 ctccaggttg taacctgcgt cataatcatt aggcgtgctg ccgctaaagc taccgagaat
    17281 aatcgccttc tgacgtggca aaattccagc atgatagagc tgcaacagca tacgttcgac
    17341 gcggaaaggg tgctcgttaa tatcttccag caccagaata ccgttctcaa tttttggcat
    17401 ccacggtgta ccaatcagtg aaatcagcat cgcaagattg cctccccaca gcgtgccttc
    17461 ggtctgacat gtcgggcctt ccccttgcca ttcaatggtg aacgttttat tgcgtaacgc
    17521 cagccagaag tggtgctcgg taaaggcatt tagttcatcc gcgccaaagt ttgctaccag
    17581 catggggccg ctaaaggtga tgacattgcc ttgcgccaga agaccgcact gaatggcggt
    17641 aaaatcgcta tgtccgcaaa tgagtaacgg gtcatgttgt tggcgagcca ccagtgcctg
    17701 ccagtcaata tctgccagta aacgactggc accgtaaccg ccgcgtacag acagcacgat
    17761 ggtgttgggg gtagtcagtc tggcaagaga attgagatct tccagacgct ccgtttccgt
    17821 acccgcaaaa cgctcacaac gacgggcaat aacctcgacg ttattgacct gatgccccgc
    17881 gtcggtaagg cgctggatac cacgcagcgc ggcgtgctgt ttaatgcagt aacccgatgg
    17941 ggcaattaag tgaaacagag acatggcaat tccttgctga caacagaaac gaaatgtata
    18001 tcatgccgct taggtgtgcc gttgtcacct caacggcgat tccaggctat aaggacagaa
    18061 gaagtgaaat tgagatggtt tgcctttttg attgtgttat tagcgggttg ttcatcaaag
    18121 catgactata cgaacccgcc gtggaacgcg aaagttccgg tgcaacgtgc gatgcagtgg
    18181 atgccaataa gccagaaagc cggtgcagcc tggggcgtcg atccacaatt gatcacggcg
    18241 attatcgcta tcgaatcggg tggtaatccg aacgcggtga gtaaatcgaa tgccattggt
    18301 ttgatgcaga taaaagcttc aacctccgga cgtgatgtat atcgtcgtat gggctggagc
    18361 ggtgagccga cgaccagcga gctgaaaaat ccggagcgta atatttcaat gggggcggct
    18421 tacctgaata ttctggaaac cggcccgctg gcaggcattg aagatccgaa ggtactgcaa
    18481 tatgcgctgg tggtgtcata cgctaacggg gcaggtgcgc tgctacggac tttctcgtca
    18541 gatcggaaaa aggcgatcag caaaatcaac gatctggatg ctgatgagtt cctcgatcac
    18601 gtagcgcgaa atcatcctgc gccgcaggct ccgcgctata tctacaaact tgagcaggca
    18661 ctggacgcga tgtaaatcag tcgcgcacat tgtccgcttt tttccgggct tctcgctcga
    18721 gagagaaaat aatccgctgt aattgccgct ccaccgtcgg gctgacgtta agaaaacgga
    18781 agctcagacg gggagtggtg atggtttcat ttttgccatc aatcactttg cgctcgctga
    18841 tggagattaa ctgggcgtca aagtgaaaaa caccccattg ccccatgttg acttcaatct
    18901 gagcgaagcg catgccttcg tgtaattcgg caggctttgc tgtttccagt aatgcgccca
    18961 tgccgcctaa cgacaaatca tacaggcgga aacgtaacgt actgttatcc gccagtttgg
    19021 tctggcaaaa ataaggcgga tggagtgggg cggtgatgcg gaaatatcgg cgtcgttgta
    19081 caaaccataa ggtgggaggc ggtacggtaa taaatgccgg aagcagcaag tattcactct
    19141 gctgtagttg ttcaacagta aactcgactt tcgcaccctg agtttcggcg gtaatggtaa
    19201 tgtgctgcgc ctttagcacg gcgatgttgt cttcggcctg gctgccgaaa tccagcacca
    19261 gtttatccgg agttattgcc agtattttgc tgatcagctg cccgccattc cagctgatac
    19321 gcaaaggaat tgcggctttg ttcaaatcgc gtaacacgcc caggacggct aacggatttt
    19381 gtttcaggaa ctgctcatgg taatgactca cgcggaaaaa ctcctgactg acaaactgtc
    19441 tttggtttat cggtcgcagt taacaaaact taatacaaat gcgcgaatat tttttacatg
    19501 ttgttcttaa atcagccgac atacgcccag catcgatccc gcccttgcct atacttagag
    19561 cgttgacgta agcatttctt gcgtccgatt catcgaaacg agggcctgaa catgggaatt
    19621 attgcctgga ttatttttgg cctgatagcc ggtattatcg ccaagctaat catgccgggg
    19681 cgtgatggtg gtggattttt cctgacctgt attcttggga tagtcggtgc ggtggtcggc
    19741 ggctggctgg cgaccatgtt tggcattggc ggctccatca gcggttttaa tttgcacagc
    19801 ttcctggtgg cggtggtggg cgctatcctg gtgctgggcg tattccgcct cctgcgaaga
    19861 gaataagatt ttcataaggc ggatagcgat acagatgccg ctatccgctt tcacatcaga
    19921 acgtatactc gataccagcc cagaaattgc gaccttcttc aataaaccct tcactgtagg
    19981 catatttggt atcaaacagg ttattgaccg acgcattaac gctgaagcca tgacctaagg
    20041 tgtaatcggc tcgaatgtgg gtcaccgcaa aaccggcggc tttttgtgaa ccgtcactgt
    20101 tgctgtagct ggaggaacgc gcctcttccg acagcgttac gcttaacggc tcccacggtt
    20161 tgagagtcat ccatgcggtc attgtctgcg ttggcagatc ggttatcttg ccgatgtctt
    20221 tgcgtttggc atcagcgtgg atcagggcgt agctcaatcc tacatccagt atattgctga
    20281 ttttcccctt aataccggca tccagaccgc tgtaatccac cgtgccgctg ttctgatttt
    20341 gaatggtatc ggcatcgata ttgtgcgaga ggatggcatc actcacccgg ttatagtaaa
    20401 cgctgacctc aaatccccag tcgtgcgtga aggcaccatt ccaggttaaa tccaccccgc
    20461 gcgcgcgttc cggcttgagc tgcgggttaa caatcgctat ctggttatac gcaggtttgg
    20521 acgtggtata gcgttctttc agcgtcggaa agcgtgtgcg gtcatagtac gaaagcgcca
    20581 gcgtgtcttc attggcaaag tggtatttcc ccatcacctg ccagttaaaa gctgactgat
    20641 tgttgtcgtc ataatgggtg atactgccat ctttctcatg ttttttcgct tctacgctat
    20701 cgcgccagtc atagctgatt ccagccacga catcgacatt atcggcagca gcccattgat
    20761 attcactggc gagcgaccag gtacgatctt catagcgatc gtaagcggcg tgcggcgcac
    20821 ctttttcccg gtgtacgtca tctttccagt taacggcaaa cgacagcaga tcgttttcac
    20881 gcacatcggc tgccagttgt aacccggcac cgtcgctgta atcggaataa tggctgtagc
    20941 tgcctttttt atttttcaaa tcagccagcg agttgtacat cattagcgta ttttcaaagg
    21001 tgtcgtgata cagccgactt ttcagggtaa aacgatcgtt tagttgggtc gttccctgat
    21061 aataaaaact ttctttgtca tactctggcc actgccagta gcgtgatttc tgaccactat
    21121 ttccactgta tggcgggtta tctttttcac catcctgctt aatgtaagtc aatgtgtatt
    21181 catcgttttc gcgtggtgta aaaccgagct tcacaatgcc gcgtttatca tcagccgatg
    21241 aattaatcat cttgccgtgt ttgcctgcaa tatcattatt tacaccatgc ggcaggccga
    21301 gaaaatcctg ctttagctgg ctaccgctga cttgcaaata ccccagatcg ctgctggcgg
    21361 caaatgaagc atgcatatcg taggcattgt cctggctacg gctccatccc tggcgatatc
    21421 ccagatttgc ttccagaggt tttgttggct tctgggtggt gatattaatg gctccgccca
    21481 tctgattagg cccctgaagc agcgacgaat accctttgga aacttcaact gcccccaaat
    21541 tgttggtcag aatccgcgcc agatcgaggt tgccgtcata gggaacataa atgggcacac
    21601 cgtcgaaata gaccggcacc tgacgactat caaagccacg aactttgacc tgttcttcgt
    21661 tgcggctacc tgacttttgc agcaccacgc cggggacgac acttaatgcc tgagcgacat
    21721 tctgtttatc cagggccttc atggtgggtt gatcgagaac ggtggtcgtc gttgatgata
    21781 ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc tgctgggtaa
    21841 aggcaagtga taatactgtg cagagataac gttttttgag tctcatagaa taaattccgt
    21901 ttaacaaaga attaaataga ggctgcgggt tgcagactga ctttaatgtc agcaggtgcc
    21961 gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta atttttgagt
    22021 gttgtcagat taattccgcc ggtcagagcc agcgtgcagt ggggagccag cgacggtgct
    22081 atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag tacgtctggt
    22141 tgcgcgcgta acgcggcgat tgcctctttc ggcgtgtcgg cttcaacaac tattttcttc
    22201 tcgggtgcgt ggcgacgtaa ctgattgatt gcgcctgacc agtcctgatt gtcatgaaga
    22261 aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg atgaatcagt
    22321 cctccggcag ccagaattgc ctgcgaggcc agtaagcggg tgcccggaat tgcttttcgg
    22381 gtgcaggcga tattgccatc agggtaacgt tcacgaagta acgccagcat ttgagcgaga
    22441 taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca tccctgatga
    22501 agtgctgcgg cattaccttg cgcacggatt agccgctgac ccgcgttcgc ttgagaaccg
    22561 tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca cgcgacagaa
    22621 attccgctga cgcagccgcc ctgacggaga aaaaactcta tatagccatg ctggtgtcca
    22681 atatttaatg ctcgcgtggt caggtcaccg ccctggatat cttccagcag taacgcatcg
    22741 atttgtgcct gagaaaggaa aatcatcgca gtgtctcctg cggtacgcta tcccaccaga
    22801 tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt tgataccagc
    22861 gatataaccg ctcacgctca tcgtcgttaa gcgcgcccag actccagctg acgttctgct
    22921 caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata aaatcaacgt
    22981 gagcataaat tcccatctga tacaggacgt tgagtgcaaa gatatagttg ggtaattcga
    23041 tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg ctgaccagat
    23101 gagtcgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgcc tggcgcatat
    23161 cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggtagg tcactccagt
    23221 cctcttccca ggcgcgctgg atccagtgga cattgtctgc ctttagcgcg gcggcgcgac
    23281 gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaatcgtc gtgagtttgt
    23341 ccgccagcgc cagcgatacg gtacccggtc cgcagcccat atcgaataag gtttgcgcgc
    23401 cttgcaggtc aatttttgag gtcagttgtt gcaggtagct gtccgtaggc gaggcgcaat
    23461 tttccgccat tttttccgcc cgttgatccc agtgatccgg cgttttttct gtgcgatggg
    23521 ccagcctgag ttgctgaagg taaaggtctg caaaatcaat atcgtcaatg agcatgattt
    23581 ttccttagtg cttaattgca gagaggtgat ggtgaatctg gtcggcagaa acccggtaca
    23641 gggcagccag ttttttcgtt gttaactgtt cggtcggtaa gccttgtgtt actcgtccat
    23701 ctggctctac ctggataatg ctgtcggcaa tggcgtttgc atggagcgga tgatgagtcg
    23761 acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga agttggatct
    23821 gatgaccgaa atcgaggctg gaggcaggct catccagtaa taacaagcgc ggttgctgga
    23881 cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta ttccagcgtc
    23941 gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgttgctttc aggcgttctt
    24001 gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac accatatcca
    24061 gcacactaaa agcaaaaatg ccgtcgtgcg cctgcggcac ccaggcaata gcctgcgcgc
    24121 gctggcgatg gctgagtgtc tgcacaggga ggctatccag taagatctcg ccttttaata
    24181 acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca ttgactccta
    24241 acagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc accggatggc
    24301 gggagtaacc cagagacgca tgacggacgg caagcaaagt cactggcgac ctccgcgtaa
    24361 caacaaaaac aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa gtgggatttc
    24421 tgttgttcca atgctgcgtg ccagcgtatc ggtaagcagg agcagaatcg cgccagtaca
    24481 cattgccatg ggcaacagct gctgatggtt atgaccggtc aacaagcggg caatgtgcgg
    24541 aacgaccagt ccgacccaac caattatgcc cgctattgct accgtgctgg cggtgatgag
    24601 tgtggcgcag acaatcaatc caaagcgcag acgtgtcacg ttcagtccca gcgaacgggc
    24661 ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca ggggcaagga
    24721 gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg tggacagccc
    24781 ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca gcgtttttat
    24841 tagcgatatc cccgctccgc acaacgtgcc taacgcaata cctaccagca ccagggttaa
    24901 gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga ccaccatcag
    24961 cccgccgcaa aatgcgtaca gctggatgta aacaatggat aatccccaca ggattgccgc
    25021 gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaca ctaagggatt
    25081 acgcaacatt ccctgatagg tggttccggc ccctgccagc gccgcgccca gaaacagcgc
    25141 agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca caggaacatt
    25201 ttcttgacga agaatgagcg ccagcagttg ctgcatatcg agatgataag ccccacttac
    25261 ggcggcaaaa gtgatactga tagctgctag cagcaaaata catccgttta cggttctcat
    25321 tagctcgcca ccaatttttg atagtcggcg tcactgagtg aacaatgcca gaacagttgg
    25381 gcgtaatgct gtatgtcact tttaaactgg cgattgatgg cgggatccag ccaggcgtga
    25441 agtctgcgta atcccagaag acggttgatt cccggcgggg catccagcca gccaaagggc
    25501 aggccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg ccagagagga
    25561 tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc cggctgccag
    25621 cgcaggagat tttccatgga aacctgtgtc agaccgtggc gatcggctat ttgcgcgacg
    25681 ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa gcccgtttcc
    25741 agccctcgag gaccgcgcgc agcataaaag ctgagattag cggcgggtga ggtggcgaat
    25801 gcctgagctt caccaacgaa gcggctggcg agattggctt gttctgcggc gcggtgctct
    25861 tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgttccag tttcccgtta
    25921 agcaataacc agggtatttg tgtctgttcg ctaacctgcc gtgcctggga gatccaggtt
    25981 tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag cccttccagc
    26041 gagagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg ggaactggcg aatgtgctct
    26101 ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac cattttttct
    26161 ggcgcgacag ccagcaatag taaatcggcg ggcgcgcctg cgctgacgat gcgtgtaatg
    26221 ttttgggctt cgggaagcgt ccgtgaggcc cagaatttga ctgattgtgc aaaggacggc
    26281 agatgatgca ggaacaatgt cgacgcaaga atctgtgtaa acctccgtct gg
//
LOCUS       DAZFRG010000043        25753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFRG010000043 DAZFRG010000000
VERSION     DAZFRG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.043"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..134)
                     /locus_tag="ACHKUC_001408"
     CDS             complement(<1..134)
                     /locus_tag="ACHKUC_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6617691.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="ACHKUC_001409"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="ACHKUC_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO6617692.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="ACHKUC_001410"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="ACHKUC_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO6617693.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="ACHKUC_001411"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="ACHKUC_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO6617694.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="ACHKUC_001412"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="ACHKUC_001412"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO6617695.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIISIDYTDFVRLTVKHSSQMAW"
     gene            complement(3777..4136)
                     /gene="tusC"
                     /locus_tag="ACHKUC_001413"
     CDS             complement(3777..4136)
                     /gene="tusC"
                     /locus_tag="ACHKUC_001413"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO6617696.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4136..4522)
                     /gene="tusD"
                     /locus_tag="ACHKUC_001414"
     CDS             complement(4136..4522)
                     /gene="tusD"
                     /locus_tag="ACHKUC_001414"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO6617697.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4522..5244)
                     /gene="yheO"
                     /locus_tag="ACHKUC_001415"
     CDS             complement(4522..5244)
                     /gene="yheO"
                     /locus_tag="ACHKUC_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6617698.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5411..6223)
                     /gene="fkpA"
                     /locus_tag="ACHKUC_001416"
     CDS             complement(5411..6223)
                     /gene="fkpA"
                     /locus_tag="ACHKUC_001416"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6617699.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKQEADAKAADSAKK"
     gene            6444..6662
                     /gene="slyX"
                     /locus_tag="ACHKUC_001417"
     CDS             6444..6662
                     /gene="slyX"
                     /locus_tag="ACHKUC_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO6617700.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6711..7301)
                     /gene="slyD"
                     /locus_tag="ACHKUC_001418"
     CDS             complement(6711..7301)
                     /gene="slyD"
                     /locus_tag="ACHKUC_001418"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6617701.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7396..7596)
                     /gene="yheV"
                     /locus_tag="ACHKUC_001419"
     CDS             complement(7396..7596)
                     /gene="yheV"
                     /locus_tag="ACHKUC_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO6617702.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7606..9411)
                     /gene="kefB"
                     /locus_tag="ACHKUC_001420"
     CDS             complement(7606..9411)
                     /gene="kefB"
                     /locus_tag="ACHKUC_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO6617703.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9411..9962)
                     /gene="kefG"
                     /locus_tag="ACHKUC_001421"
     CDS             complement(9411..9962)
                     /gene="kefG"
                     /locus_tag="ACHKUC_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO6617704.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGC"
     gene            10093..12006
                     /gene="yheS"
                     /locus_tag="ACHKUC_001422"
     CDS             10093..12006
                     /gene="yheS"
                     /locus_tag="ACHKUC_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6617705.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12006..13028
                     /gene="yheT"
                     /locus_tag="ACHKUC_001423"
     CDS             12006..13028
                     /gene="yheT"
                     /locus_tag="ACHKUC_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6617706.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13022..13240
                     /gene="yheU"
                     /locus_tag="ACHKUC_001424"
     CDS             13022..13240
                     /gene="yheU"
                     /locus_tag="ACHKUC_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO6617707.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13294..14163
                     /gene="prkB"
                     /locus_tag="ACHKUC_001425"
     CDS             13294..14163
                     /gene="prkB"
                     /locus_tag="ACHKUC_001425"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO6617708.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14218..14622)
                     /gene="yhfA"
                     /locus_tag="ACHKUC_001426"
     CDS             complement(14218..14622)
                     /gene="yhfA"
                     /locus_tag="ACHKUC_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO6617709.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14924..15556
                     /gene="crp"
                     /locus_tag="ACHKUC_001427"
     CDS             14924..15556
                     /gene="crp"
                     /locus_tag="ACHKUC_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO6617710.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15607..17697
                     /gene="yhfK"
                     /locus_tag="ACHKUC_001428"
     CDS             15607..17697
                     /gene="yhfK"
                     /locus_tag="ACHKUC_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO6617711.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17775..18995)
                     /gene="argD"
                     /locus_tag="ACHKUC_001429"
                     /gene_synonym="dapC"
     CDS             complement(17775..18995)
                     /gene="argD"
                     /locus_tag="ACHKUC_001429"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO6617712.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPVSAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19081..19644)
                     /gene="pabA"
                     /locus_tag="ACHKUC_001430"
     CDS             complement(19081..19644)
                     /gene="pabA"
                     /locus_tag="ACHKUC_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO6617713.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19676..20278)
                     /gene="fic"
                     /locus_tag="ACHKUC_001431"
     CDS             complement(19676..20278)
                     /gene="fic"
                     /locus_tag="ACHKUC_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO6617714.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20268..20435)
                     /gene="yhfG"
                     /locus_tag="ACHKUC_001432"
     CDS             complement(20268..20435)
                     /gene="yhfG"
                     /locus_tag="ACHKUC_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO6617715.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMRLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20540..21112)
                     /gene="ppiA"
                     /locus_tag="ACHKUC_001433"
                     /gene_synonym="rotA"
     CDS             complement(20540..21112)
                     /gene="ppiA"
                     /locus_tag="ACHKUC_001433"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO6617716.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21383..22564
                     /gene="tsgA"
                     /locus_tag="ACHKUC_001434"
     CDS             21383..22564
                     /gene="tsgA"
                     /locus_tag="ACHKUC_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO6617717.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22826..25369
                     /gene="nirB"
                     /locus_tag="ACHKUC_001435"
     CDS             22826..25369
                     /gene="nirB"
                     /locus_tag="ACHKUC_001435"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO6617718.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25366..25692
                     /gene="nirD"
                     /locus_tag="ACHKUC_001436"
     CDS             25366..25692
                     /gene="nirD"
                     /locus_tag="ACHKUC_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO6617719.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
BASE COUNT         6299 a         6964 c         6440 g         6050 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcgc gacctttggt
      301 cagagaacgc agctgagttg catatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttcagg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgattgcagc tgcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtacacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgctcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatac gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccaccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atggaaatga tgtcgttcga aattcgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agagcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt gccgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtcac cttcactgag cagacgcagc
     3721 agcgcagcaa agtccgtcag ccagggtgaa cgatgtaatg tgtgcagcat aacgcctcaa
     3781 aacctcaaaa taacatcgta gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc
     3841 gtggcttcga ccacaaaggg tgtctgcgga tctaacccac gttcgcgcag tgaagccgcg
     3901 caaatccagc actgttcaat atcgtacaga cccaacaatt taaaagtggc aatgtaatca
     3961 cgcgccagca ctgcatcggg cttttgtcct ggcagcagct gaaaaacgcc atcagcaata
     4021 aagaagacgg ccagatcgtc agttaatgcg gaagttgcca gtaaagcatc taaaccttcc
     4081 cggcctgcag ttgtaccatg aggcgcagta gaaaaaacaa acgcaattcg tttcatcaga
     4141 actgtaccac cctgtcacag gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg
     4201 taaatcccgg ctgaaggttt gacgaagcga gccccagtct tccggcctcc gtttcatcaa
     4261 caacgccacg gcgtaatgct gccgctacgc agatattcag cgccacacca tgttgcgcat
     4321 tcaactgttg ccagccgcgc acaaggtcaa attcgtcgct tgccggtgag gtcaattggt
     4381 tagcgttata gaccccttcc cgatagaaaa aaacgctgct taactcatgg ccttctgcta
     4441 tcagcgcctg cgcaaactga aaagcactac tcgcctgttg cgtaccgtat gctggcccgg
     4501 tcaccacgat ggcaaaacgc attacttatc ttgcccctgg aaatcaccgc tcttgaactg
     4561 gcggatgtag agatagacag tgtgtttgga gatgttcagg cggtcagcaa cctggttgat
     4621 cgcgtcttta atatcgaaga tccctttctc gtagagattt agcacgatct gacgattttt
     4681 ggcgttatta gaaacattgc gatcggcatt cacttcttcg atggtgaact ccagcgtttg
     4741 ggtaaccaga tcttcaacag aagaggcaaa gttgacgctt gaaccgacat ccggagtttc
     4801 cggcggcaca aaagtgctca taatctgcga gaagggaaca tcaagattca tattgatgca
     4861 cagcagacca attacacgct gttcgcggtt acgaatcgcg atagtcaggg acttcattaa
     4921 tacgccgctt ttggcgcgag taaagtagca tttagaaacg ctgctatccg ctcccgtcat
     4981 atcgtgcagc atacgtagcg ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg
     5041 ttcaccgtta gcaatgcgaa tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat
     5101 ttcacagtgg gagccaataa gcatcgctaa cccgtccacc accgcttcgt aggatttcag
     5161 aatatcaaaa tcggtctggt caaaaggacg ttgatccagt aaatccaact cactggtttc
     5221 gttggttaaa agcgacctgg acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa
     5281 cttttcaggg caggctcatt aatgatgcgg gtaactaaat taatacagcg gaggttccgc
     5341 tttccagcac taattatatc cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg
     5401 ttcttaatgc ttatttttta gcagagtctg cggctttcgc atcagcttcc tgctttgcat
     5461 cagccttcgg cgctggtttc acatccagca gctctacgtc aaacaccagg gtagaattcg
     5521 gtgggatccc cggaacaccc gctttgccgt aagccagttc tggtggaata accagtttga
     5581 tcttaccgcc tttcttgatg ttcttcagac cttctgtcca acccgggata acaccgtcca
     5641 gacggaaaga gagcggttca ccacgggtgt aagagttgtc gaactcttta ccgtcgatca
     5701 gcgtaccttt gtagttcact acaacagtat cgctgtcttt cggtgcttcg cctttaccgg
     5761 cttctactac ctgataaacc agaccagtgg aagaggtttt cacacctttc tctttggcaa
     5821 atttctcgcg gtactcttta ccttttgctt cgttatcagc cgcgtctttt tccatcttcg
     5881 cctgagcaga agacttcaca cgagcttcga atgcttgcag agtctgttcg atctcttggt
     5941 cggaaagttt gctcttatca gcaaatgcat cctgaacacc agcgatcagc tgatctttat
     6001 ccagtttgat gcccagtttt tcttgttctt taagagagtt ttccatgtaa cgacccagcg
     6061 aagcacccag tgcataagct gatttctgat cgtcattttt gaacgctgct ttgctgtcag
     6121 cagttgtagc aggttttgca gcttcagcag caaaagtgat tggtgcatgc agggcaacgg
     6181 ccattgtggt cgccagcagc gttactttaa acagtgattt catccatatc tccaggatcg
     6241 gggcatctca ccccagggtt aactattatc agaagggtac tataaagcgt tgtcgaacaa
     6301 atctacatac agacacgccc tattatcatc tattttcaga ctctttttgt ttaaattagt
     6361 ttcgatgacc gcgaaatgag tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga
     6421 ccattcgaca aaagaggtga atcatgcagg atttatcatt ggaagcacgc ctggcagagc
     6481 tggagagccg attggctttt caggaaatca ccattgaaga actgaacgtc acggtgaccg
     6541 ctcatgaaat ggagatggcg aaactgcgcg atcatctgcg tctgctgacc gagaagctaa
     6601 aagccagcca gccgtcgaac atcgcgtcgc aggctgaaga aacgccaccg ccacattatt
     6661 gaggcgtaaa aaaagcgggg attccccgct tttttgtcac cttttcggta ttagtggcaa
     6721 ccgcaaccgc cgttgccttt accgccacag cagccttcgc caccgtgttc atgaccgtga
     6781 tcgtggccat gaccaccgca gcaaccgtcg tggtcgtgat cgtggtggtg atcgtgcgcg
     6841 ccgtgaacgt gaccatgagc cagttcttct tcggtcgctt cgcgaatcgc cacaacttca
     6901 acgttgaatt tcaggttctg accagccagc atgtggttac catcaaccac tacgtgatcg
     6961 tcttcaaccg cagtgatttc aaccggtacc ggaccctggt cggtttcagc caggaaacgc
     7021 atacctacct gcagttcatc aacgcccata aatacgtctt taggaacgcg ttgcaccagg
     7081 ttttcgtcgt actgaccgta agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca
     7141 acttcatgac cttccagcgc tgtttccagg ccagagatca gggaaccgtg accatgcagg
     7201 tagtccagcg gcgcactcac cggagactca tcaaccaaca caccgtcttc tgtacgtacc
     7261 tgataggcca ggctgaccac caggtctttt gctactttca tgatatctcc tgagcatggg
     7321 aagaatagtg gcgcagattg tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa
     7381 actcggcgca tatcgctagt ccggatgaaa aatcccgatc acttgctcat ctttgcggac
     7441 gtgatcgcgg gcttctttgt ctgcttctcg catctgatgt ccacacttaa cacattctac
     7501 gatatcaata ttattttcgc gccacatcgc cattgaatcc tgtgcctgac aggccgggca
     7561 ttttgcgccc gcaataaaac gttttcggat tgccatcttt accctctact caaattcatc
     7621 ccagccgtcc agctggcgtc gttcttgttg catttcacgc tggaaaatct cttccagttc
     7681 acgtcgggct tccctggcgc gagaaatttg tacggtatca gcatgcatcg ggattaactc
     7741 ccgcagcatt cgcatatcca ggcggcgaaa atgcagttgc gcgcgctgcg cctgatgcgg
     7801 atgcatgcca agcgtgacca gcgtcttgcg ccccagctct aacgcactgg agaatgtttc
     7861 acgggaaaac tgcgtcaccc ctgcctgtaa taactcatgc gcttccacac gtccgcgcgc
     7921 tcgcgcaaga atatgcaaat gcggaaagtg ctgctggcat atttccacca gcttcatagt
     7981 gtcttccggc tcgttacagg taatgacgat agactcagcg gcctctgcac ccgcagaacg
     8041 taaaagatct acctgcgtgg cgtcgccgta ataaactttg tagccatatt tgcgcatcag
     8101 gttaacggca ctgatatccc gctccagtac agtaatgcgc attttatttg ccatcagcaa
     8161 acgaccaatc acctgaccaa aacgcccaaa gcccacgaca atgacctgcg gtttatcatc
     8221 gttgacccat ggtttttcgt cttcttcttc cggtccgtta aactggcggg atagccattt
     8281 atcgaccagc ttcatcagca gcggcgtggt catcatggaa agcgtcaccg tcaccagcaa
     8341 cagcgccatc tggtcgccct ggaataagcg ttgtgaagaa gcggtagaaa agaggacaaa
     8401 ggcaaactca ccgccctgac tcaacacgcc agcaaactgc atccgctcag aactgcgcac
     8461 gccatacaat cgcgccagca gatacagcac gagaattttc accgccacca gcacaaccac
     8521 acttatcact acccacaaca gatgggtata aagcaccccg aggttgagcg acatgccgac
     8581 agagataaag aacaaaccaa gcagcaagcc tttgaaggga tcgatagccg tttccagttc
     8641 atggcgatat tcactttccg ccagtagcac acccgcaata aacgtaccga gcgccatcga
     8701 caaccccagc gcatccataa acaatgcgga acccaacacc agcagcagcg tcgcggcggt
     8761 gaacacttcc cgcacaccag aagctgcaat aaaacggaat accggacgca gtaaatagcg
     8821 cccgccaatc agcatgccga caaacgccag caccttcatg ccgatcttca tccagtcgaa
     8881 atgttcgtct gccgaacccg ccaacaacgg cactaacgcc agtgccggga tcaccgccag
     8941 atcctgaaac agcagtaccg agaaacctaa ctggccggat tcgctgcgat tcatcccttt
     9001 ctcacgcatc aattgcaacg ccattgcagt tgaagacatc gcaaggccaa tgccaccgac
     9061 caccgccgcc tgccatgaga aatgggtcag catcaataat cctgccagca acgccgcgct
     9121 taacagcacc tgtgccgcgc ctacgccaaa aatcgaacgc cgcagttgcc aaagcttgga
     9181 gggattcaac tcaaggccga tgataaacat caggaatacc acgccgagtt ccgaaaagtg
     9241 gaggatctca tcgacgtcgc taataaaccc cagcccccac gggccaatcg caatccccgc
     9301 cagcaaatat cccaacactg caccaatacc cagccgcgat gccagcggca ccgcagccac
     9361 cgccgcgaag agaaacagca ctcctgcgag taaaaaatca gaaccttcca tcagcagcct
     9421 cctggagaca gcggatttgc cagccagtca ccgtaggctc tggcgtggct cgccagctcc
     9481 tgtgcgcttt gccgtctcgc ccagtaaata atgatggggc ttaaccaatg catccggcac
     9541 atgcccgccg ccagttcaaa ggggcgcaga acatcgctca tcgggtagcg attcaacgcg
     9601 tcataacggt aagcactttc cggctccccg gtggtaatca cgctacgcca gtactttccc
     9661 gccagttggt ttccccccgg tccgctggca aaaccacggc ttaatacccg gtccagccac
     9721 tctttcagta gcgccgggca gctataggta taaagaggat gctgaaagac aatcacctcg
     9781 tgctcgcgca gtaatgcctg ctcacgaggg atatcaataa aaaagtcggg atagtgcgcg
     9841 taaaggtcgt gcacggtaac attgctgagc tgcgtggccg gtttaagcag aacccggttt
     9901 gccaccgagt cctgagattc cggatgggca tacagcagca aaactttcgc tggctgagac
     9961 atcatccccc tcccggtatg gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc
    10021 cggtgcggca gctcgcccat acattacatt atcataatga taagttaaca tagtctgaac
    10081 atacggcgcc ttatgattgt tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg
    10141 ctggataatg ccaccgccac catcaaccct gggcagaaag tcggcctagt gggtaaaaac
    10201 ggctgtggta aatctaccct gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc
    10261 agctacacct ttccgggaag ctggcaactg gcgtgggtga atcaggaaac accggcgtta
    10321 ccgcaagcgg cgctggaata tgtcattgac ggcgaccgtg aatatcgtca actggaagcg
    10381 cagctacacg acgccaacga acgtaacgac gggcacgcca ttgcgaccat tcatggcaag
    10441 ctggatgcta ttgacgcatg gagtattcgc tcccgtgccg ccagcctgct gcacggcctc
    10501 ggtttcagca atgaacaact ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg
    10561 cgtcttaacc ttgcccaggc gctgatttgc cgttcagact tgctgctgct cgacgaaccg
    10621 actaaccacc tcgatctcga tgccgttatc tggctggaaa aatggctgaa gagctatcag
    10681 ggcacgctga tcctgatctc tcacgatcgc gatttcctcg atccgatcgt tgataaaatt
    10741 attcatatcg aacaacaaag catgttcgag tacaccggca actacagttc gtttgaagta
    10801 cagcgcgcca cccgtctggc gcagcaacaa gcgatgtatg aaagccagca ggaacgcgta
    10861 gcgcatctgc aaagttatat cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc
    10921 cagagccgca ttaagatgct tgagcgtatg gagctgattg cccccgcgca cgtcgacaac
    10981 ccgttccgct ttagcttccg cgcgccggaa agcctgccaa atccgttact gaagatggaa
    11041 aaagtcagcg caggctatgg cgatcgcatt attctcgact cgattaaact gaatctggtc
    11101 cccggctcgc gcattggtct gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa
    11161 ctgttagccg gggaacttgc gccagtcagc ggtgaaattg gtctggcgaa agggatcaag
    11221 ctcggctact tcgcccagca tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa
    11281 catctggcac gtttagcgcc gcaggagctg gagcaaaaac tgcgtgacta cctcggcggc
    11341 tttggtttcc agggcgataa agtaaccgaa gaaacgcgcc gcttctccgg tggggaaaaa
    11401 gcccgcctgg tgctggcatt aattgtctgg cagcgtccga atctgctgct gctcgacgaa
    11461 ccgaccaacc accttgacct cgacatgcgt caggcactca ccgaagcatt aatcgagttc
    11521 gaaggcgcgc tggttgtcgt ctcgcacgac cgtcatttgc tgcgttccac cactgacgat
    11581 ctctacctgg ttcacgatcg taaagtcgaa ccgttcgacg gcgatctgga agattatcaa
    11641 cagtggttga gcgacgtaca aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac
    11701 gcgaacagcg cccaggcacg taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa
    11761 acccagccac tgcgtaaaga gattgcccgt ctggaaaaag agatggagaa gctgaacgcg
    11821 caactggcgc aggcggaaga gaaactcggc gacagcgaac tgtacgatca aagccgtaaa
    11881 gcggagttga ccgcctgcct gcaacagcaa gccagcgcca aatccggcct ggaagagtgc
    11941 gaaatggcat ggctggaagc ccaggagcag cttgagcaga tgctgctgga aggccaaagc
    12001 aactgatggc gcagataaca acgaccgatg ccaatgaatt tagcagcagt gctgaattca
    12061 cccccatgcg cggctttagc aattgtcatc tacaaaccat gctgccgcgt ctgttccgtc
    12121 gcaaggtgaa attcaccccg tactggcagc ggctggagtt gcccgacggc gattttgtcg
    12181 atctcgcgtg gagtgaagat cctgcacagg cgcggcataa accgcgttta gtggtatttc
    12241 acgggctgga aggcagtctc aatagccctt acgctcacgg tctggttgag gcggcgcaaa
    12301 agcgcggctg gctgggcgtg gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta
    12361 tgcaccgcat ttaccattcg ggcgaaaccg aagacgccag ttggttttta cgctggctgc
    12421 aacgcgagtt tggtcatgcg ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc
    12481 tggcctgttt gctggcaaaa gaaggcaatg atctcccgat tgatgcggca gtgattgtct
    12541 ctgcgccgtt tatgctggag gcctgtagtt accatatgga aaagggcttt tcccgcgttt
    12601 atcagcgtta cttgctgaac ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc
    12661 ccggcacact gccgattaat ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg
    12721 acgatctcat caccgccaga attcacggct acgctgacgc tatcgactat tatcgtcagt
    12781 gtagcgccat gccgatgctc aatcggatag ccaaaccgac actgattatt cacgccaaag
    12841 acgatccgtt tatggatcat caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg
    12901 agtatcagct gactgaacat ggcggtcatg ttggctttat tggcggtaca ttacttcatc
    12961 cgcaaatgtg gctggagtca cgcattcctg actggttaac aacgtatctg gaggcgaaat
    13021 catgttgatt ccgtggcaag acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt
    13081 tgtgttacgt gaaggcaccg attatggtga acatgagcgt acacttgaac agaaagtcgc
    13141 cgacgtcaaa cgccagctac agtgcggaga agcggtgctg gtatggtcgg agctgcacga
    13201 aacggtcaat atcatgccgc gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg
    13261 agataataat gatagctaag tcatggagtt actatgtctg ccaaacatcc ggtcattgcg
    13321 gtaacaggat ccagcggcgc ggggaccacc accaccagcc tcgcgtttcg taaaatattc
    13381 gcgcagttaa atctgcatgc agccgaggtg gaaggcgaca gttttcaccg ttatacccgc
    13441 ccggaaatgg acatggcgat ccgcaaagcg cgcgacgccg ggcggcatat cagctacttc
    13501 ggccccgaag cgaacgactt cggccttctg gaacaaacct tcattgaata cggtcagagc
    13561 ggcaaaggga aatctcgcaa atatctgcat acctacgatg aagccgtacc gtggaatcag
    13621 gtaccgggga cattcacccc ctggcaatct ttaccggaac ccactgatgt actgttttat
    13681 gaaggtttac acggcggcgt agtcacgcca cagcataacg ttgcgcagca tgtggactta
    13741 ctggtcggcg tggtgcctat cgttaacctt gagtggattc aaaaactgat ccgtgacacc
    13801 agcgagcgcg ggcactcacg agaagcagtg atggactcgg tagtgcgttc aatggaagac
    13861 tatatcaact acatcacacc gcagttttcc cgcacccatc ttaacttcca gcgcgttccc
    13921 accgtcgaca cctcaaaccc gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt
    13981 gtggtgatcc attttcgtaa tctggaaggg atcgatttcc cctggctgct ggcgatgttg
    14041 caaggctcat tcatttccca catcaatacg ttagtggtgc ctggcggcaa aatgggtctg
    14101 gcaatggaat taattatgct gccgctggtg caacgattga tggaaggaaa gaaaatcgag
    14161 taacgctgct attacgccgg ataaaatact atccggcttc acaacgggat agttaaatca
    14221 cgcggcaacc acttcatacg agtgagtaat attcaccgct ttttccagca tcagcgccac
    14281 tgaacaatat ttctcggcag agagatcaac cgcacgcgca accgctgcgt ctttcaggtc
    14341 gcgaccggtg acgataaaat gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg
    14401 gcgctcagag gtcaatttta cttcacaatc gaccacatcc tgacgccctt tttgcaggat
    14461 agaaaccaca tcgatggcac tgcaaccacc cgccgccatc aacaccattt ccatcgggct
    14521 tggtgcttta tcgcctgagt tgccgtccat taaaatctgg tgaccagagg cggattcgcc
    14581 cagaaaagtt aacccttcga cccacttcac tcgcgcttgc atttttgcta ctccactgcg
    14641 tcaattttcc tgacagagta cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt
    14701 catgctgaag cgagacacca ggagacacaa agcgaaagct atgctaaaac agtcaggatg
    14761 ctacagtaat atattgatgt actgcatgta tgcaaaggac gccacattac cgtgcagtac
    14821 agttgatagc cccttcccag gtagcgggaa gcatatttcg gcaatccaga gacagcggcg
    14881 ttatctggct ctggagaaag cttataacag aggataaccg cgcatggtgc ttggcaaacc
    14941 gcaaacagac ccgactctcg aatggttctt gtctcattgc cacattcata agtacccatc
    15001 caagagcacg cttattcacc agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg
    15061 ctctgtggca gtgctgatca aagacgaaga gggtaaagaa atgatcctct cctatctgaa
    15121 tcagggtgat tttattggcg aactgggcct gtttgaagag ggccaggaac gtagcgcatg
    15181 ggtacgtgcg aaaaccgcct gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt
    15241 gattcaggta aacccggaca ttctgatgcg tctgtctgca cagatggcgc gtcgtctgca
    15301 agtcacttca gagaaagtgg gcaacctggc gttcctcgac gtgacgggcc gcattgcaca
    15361 gactctgctg aatctggcaa aacaaccgga tgctatgact cacccggacg gtatgcaaat
    15421 caaaattacc cgtcaggaaa tcggccagat tgtcggctgt tctcgtgaaa ccgtgggacg
    15481 cattctgaag atgctggaag atcagaacct gatctccgca cacggtaaaa ccatcgtcgt
    15541 ttacggcact cgttaatccc gtcagagtgg cgcgttacct ggtagcgcgc cattttgttt
    15601 cccccgatgt ggcgcagact gatttatcac cccgatatca actatgcact tcgacaaacg
    15661 ctggtgctat gtttgcccgt ggccgttggg ttaatgcttg gcgaattacg attcggcctg
    15721 ctcttctccc tcgttcctgc ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt
    15781 tttttcaaac gcttaatcat tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag
    15841 gtgctactgg caaaagatgt tcccctgccc tttttgctga ccggattaac gctggtactt
    15901 ggcgtcactg ctgagctggg gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc
    15961 gccattttta ccctcagtct ggcgggatac atgccggtct gggaaccgtt gctcatctat
    16021 gcgctgggca ctttgtggta cggattgttt aactggtttt ggttctggat ctggcgcgaa
    16081 caaccgctac gcgagtcact aagtctgctg taccgtgaac tggcagatta ctgtgaagcc
    16141 aaatacagtc tgcttaccca gcacaccgac cctgaaaaag cgctgccgcc gctgctggtg
    16201 cgccagcaaa aagcggtcga tttaattacc cagtgctatc agcaaatgca tatgctttcc
    16261 gcgcaaaata ataccgatta caagcggatg ctgcgtattt tccaggaggc gctggatttg
    16321 caggaacata tttcggtcag tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt
    16381 agccatgcgg aagaagttat ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg
    16441 ctggctgatg acattcttta ccatcgcctg ccaacgcgtt ttacgatgga aaagcaaatt
    16501 ggcgcactgg aaaaaatcgc ccgccaacac ccggataatc cggttgggca attctgctac
    16561 tggcatttca gccgcatcgc tcgtgtgctg cgcacccaaa aaccgctcta tgcccgtgac
    16621 ttactggccg ataaacagcg gcgaatgcca ttacttccgg cgctgaaaag ttatctgtca
    16681 ctaaagtctc cagcgctacg caatgccgga cgactcagtg tgatgttaag cgttgccagc
    16741 ctgatgggca ccgcgctgca tctgccgaag tcgtactgga tcctgatgac agtattgctg
    16801 gtgacgcaaa atggctatgg cgcaacccgt ctgaggattg taaatcgctc cgtgggaacc
    16861 gtggtcgggt taatcattgc aggcgtgacg ctacacttta aaattcccga aggttacacc
    16921 ctgaccttga tgctgattac caccctcgcc agctacctga tattgcgcaa aaactacggc
    16981 tgggcgacgg tcggttttac tattaccgca gtgtataccc tgcaactgtt gtggctgaac
    17041 ggtgagcagt acatccttcc gcgtcttatc gataccatta ttggttgttt aattgctttc
    17101 ggcggtactg tctggctgtg gccgcagtgg cagagcggat tattgcgtaa gaacgcccat
    17161 gacgctttag aagcctatca ggacgcgatt cgcttgattc tgagcgagga cccgcaacct
    17221 acgccactgg cctggcagcg aatgcgggtg aatcaggcgc ataacactct gtataactca
    17281 ttgaatcagg cgatgcagga accggcgttt aacagccatt atctggcaga tatgaaactg
    17341 tgggtaacgc acagccagtt tattgttgag catattaatg ccatgaccac gctggcgcgg
    17401 gaacaccggg cattgccacc ggaactggca caagagtatt tacagtcttg tgaaatcgcc
    17461 attcagcgtt gtcagcagcg actggagtat gacgaaccgg gtagttctgg cgatgccaat
    17521 atcatggatg cgccggagat gcagccgcac gaaggcgcgg caggtacgct ggagcagcat
    17581 ttacaacggg ttattggtca tctgaacacc atgcacacca tttcgtcgat ggcatggcgg
    17641 cagcgaccgc atcacgggat ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac
    17701 aggctaaacc atcggatgag gtgttcacgc cgcatccgac atcttttgcc tgatgcgacg
    17761 ctgacgcgtc ttatttacgc cccaatcacc ttcgccaccg cgtgggcgaa acgatgcatc
    17821 ccttcatcaa tatccgcatc ttccaccacc agcgacggcg caaaacgcat cacatcaggc
    17881 ccggcattca gcaccattac gccttcctct gcacccgcat acaggaaatc acgcgcccga
    17941 cctttgtact gtggtttcag ctctgcgcca atcaacagcc ccataccgcg aatatcgcta
    18001 aacacatcgt actgctgatc gatcttctgc aaatggtcaa caaaacgctg gcgtttcgcc
    18061 tgaatacctt ccagcacttc aggggtattg atgatatcaa acgctgcgcc cgctactgca
    18121 caggccagag gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcggaagca
    18181 atctccgccg tggtcagcat ggcgctaacc gggaaaccgc cacctaacgc tttcgcagag
    18241 gtcagaatat ccggcgtcac gccgtagtgc atataggcaa acagatcgcc ggtccgcccc
    18301 atcccgcact gcacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc
    18361 aagccctgca aaaactctgg cgtcgctgcc gtcacgccgc cctcgccctg aatcggctca
    18421 accaccaccg cacaggtgtg atcatccatc accgctttca ctgcgtggag atcgttaaag
    18481 ggaacgtgga tgatgtctgc cggttttgga ccaaagccgt cggaatattt tggctgccca
    18541 cccaccgaaa cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg
    18601 gttttgaacg ggctatgacg cacacaggcg taatggcgtg ccagtttaaa ggcggtttcg
    18661 ttagcttccg tgccggagtt cataaacacc acgcgttcgg caaacgttgc ctcaatcagt
    18721 ttacggccaa gacgcagcgc cggctcattg gtgaaaacgt tactaatatg ccacagagtt
    18781 tcgccctggg tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact
    18841 gcaatgccac ccgcgaaatc gacatactcc ttgccctgtt gatcccagat tcggctgccc
    18901 tgacctttta ccggaataaa ctctgccgga gcataaatcg gcagaatcac ttcatcgaaa
    18961 gtcgcgcgtg taattgctgt ttgttcaatt gccatctcat gatcaccctg ttacgcataa
    19021 ataaatatga aattataatc acaaaatatg cataaaaaat cactgaatgg caatcagaaa
    19081 tcagcgatgc agaaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg
    19141 atggaattgc acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt
    19201 ttcgctccag gccgtcactt caaagcactc cggtaatgag tcaggttcca ctaccagcga
    19261 atggtagcgt gtcacggtga gtggatttgc cagcccctta aatacgccca caccattatg
    19321 tgtaatcggc gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt tgccgccaaa
    19381 tgcctgcgcc attgcctgat gaccgaggca gacgccaaga atcggcaagc gtccggcata
    19441 gtgacgaata acgtcaaggg agatcccggc ttcatctggc gtacaggggc cgggtgagat
    19501 gacaattttt tgtggtttaa gggcgtcgat atccgccaac gtcaacgcat cgttgcgctt
    19561 aaccaacaca tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc
    19621 gtagttatca ataagcagga tcatggcggc tccggtacga aagaaccgcg ctattttact
    19681 cagattcccc ggcttcgctt accactttgc taaatatcat ctgcagcgcg gtgagatccc
    19741 ccattgcccc attctgattt gcctgattcc aggcctcttt ttcaataccc tgccagctca
    19801 gttgatatcc ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc aaaccacttc
    19861 ccacccggaa aggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga
    19921 acttcgcttt ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat
    19981 tgccctcttt ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcga
    20041 cttcgcgcag ttgccccgcc cagtcgaaaa tatcctgata caactggcga tgaagagtac
    20101 gcaaatgcgg taaaccccga accaacgggc caagttcaat ggtcgcagca cgcagcgccg
    20161 tcatttcgta agcggcctgt tccagccgct gctgctggcg gatgttcagc cggttacgca
    20221 tgatatcaag gcctggataa agatacggat cgcgcccttc gccgaattta tcgctcatag
    20281 tgcctcctca gcgcttcaag gcgcgctaaa gcctctgcgg cagtaagagt gactaaacgc
    20341 atctcgacgc cttcaagacg gcgactggcc tggaaattac gattacgctg aagctcccag
    20401 agacgggact tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat
    20461 ttgccacaag tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga
    20521 ttgcccgcgc gagaaatcat tacggcagga ctttagcgga aaggataact accggttttg
    20581 acggcacatt ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc ttatcggcaa
    20641 cgtccatgcc tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccgtggt
    20701 caaggaaggc gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag
    20761 cggtacgcgc catcgcgatg gtgccacgcg tgttgcgcag gccgttatct gcttcatttt
    20821 tgattggcgg gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca
    20881 taaagccagg aatgacgcgg tgaaaggtcg tgttgttata aaaaccactg ttcacataat
    20941 cgacaaagtt ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac
    21001 cagctgaggt tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa
    21061 gagcagaaag agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc
    21121 cttaacaaac agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa
    21181 ggccatccct ttacctaatt ttacgtatct gaaatatatg taacctgagg ataacttttt
    21241 attccgtgat caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt
    21301 taatcagatc acaaaatcgc ctaaaatcgc ccgctcgcgg catagcccgc tatgccattt
    21361 tatttttgaa cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag
    21421 ctttctttcc tacgcactga ccggtgcgtt ggttattgtc accgggatgg tgatgggaaa
    21481 tatcgccgat tatttcaatc tgcctgtttc cagcatgagt aacaccttca ccttcctcaa
    21541 tgccggcatt ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa
    21601 aacgcagtta cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag
    21661 ccacagcctg gcgctgttct cggcggcgat gttcattctc ggggtggtca gcggcatcac
    21721 catgtcgatt ggtacattcc tggtaacaca aatgtatgaa ggacgtcagc gcggttcacg
    21781 cctgttattt accgactcct tcttcagtat ggccgggatg attttcccaa tgatcgccgc
    21841 gtttctgctg gcacgtagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta
    21901 tgttgctatt tttattctga ccttcggctg tgagttcccg gcactgggca aacatgcgcc
    21961 aaaaacagat gctccggtag agaaagaaaa gtgggggatc ggcgtactgt ttctctctgt
    22021 tgcggcgctg tgctacatcc tcggtcagtt aggttttatc tcctgggtgc ctgagtatgc
    22081 caaaggcctg ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc
    22141 atacatggtc ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat
    22201 tctgaccgta ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc
    22261 ggcacatatg gcgtggtcaa ttctcgccct gggcttcttc tccagcgcga tctataccac
    22321 catcatcacc ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt
    22381 cctgacctgc gggaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga
    22441 acatagtggt ccgcaggcgg cgctgctgac ggcaaacggt ctgtacgctg tcgtctttgt
    22501 gatgtgcttc ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca
    22561 ttaattgttc atgccgggcg gcactatcgt cgtccggcct tttcctctct tactctgcta
    22621 cgtacatcta tttctataaa tccgttcaat ttgtctgttt tttgcacaaa catgaaatgt
    22681 cagacaattc cgtgacttaa gaaaatttat acaaatcagc aatatacccc ttaaggagta
    22741 tataaaggtg aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg
    22801 taatagaaaa gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg
    22861 tatggtcggc catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga
    22921 tattaccgtt ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta
    22981 cttctctcac cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca
    23041 cggcatcaaa gttctggtcg gcgaacgcgc tatcaccatc aaccgtcagg agaaggtgat
    23101 tcactccagc gccggacgta ccgtttttta tgacaagctg atcatggcga ccggttccta
    23161 cccgtggatc ccgccaatta aaggttctga tactcaggac tgctttgtct atcgcaccat
    23221 tgaagacctc aacgccattg aatcctgcgc tcgtcgcagc aagcgcggtg ccgttgttgg
    23281 tggcggcctg ttaggtctgg aagctgctgg cgcgctgaaa aacttaggca ttgaaaccca
    23341 cgttatcgaa tttgccccta tgctgatggc ggaacagctt gatcagatgg gcggcgagca
    23401 gctgcgtcgc aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acactcttga
    23461 gattgtgcag gaaggtgttg aagcgcgtaa aaccatgcgt tttgccgacg gcagcgaact
    23521 ggaagtcgac tttatcgtct tctctaccgg tatccgtccg cgcgataagc tggcaaccca
    23581 gtgtggtctg gacgttgctc cgcgtggggg tatcgtcatc aatgattcct gccagacttc
    23641 cgatccggat atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct
    23701 ggtagcacca ggctacaaaa tggcgcaggt cgccgttgac catattcttg gtagcgaaaa
    23761 cgcctttgaa ggtgcagacc ttagcgccaa gctgaaactg ctgggcgtag acgtaggcgg
    23821 tattggtgat gcgcacggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag
    23881 caaagagatc tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt
    23941 actggtgggc gataccagcg actacggcaa cctgctgcaa ctggtgctga acgctatcga
    24001 gctgccggaa aacccggatt ccctgatcct gccggcgcac tcgggtagcg gcaagccgtc
    24061 tatcggtgtt gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcaccaa
    24121 aggcgacctg attgctgcta tcaacaaagg ctgccacacc gttgcggcgc tgaaagccga
    24181 aaccaaagcg ggtactggct gcggtggctg tatcccgctg gtcactcagg tactgaacgc
    24241 ggaactggcg aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc
    24301 gcgtcaggaa ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct
    24361 ggcgaaacac ggcaaaggtt acggttgtga agtttgtaaa ccgaccgtcg gttcgctgct
    24421 ggcctcctgc tggaacgaat acattctgaa gccggaacat actccgctgc aggattctaa
    24481 cgacaacttc ctcgcgaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc
    24541 gggcggtgaa atcaccccgg aagggctgat ggcagtgggt cgtatcgcgc gtgaatttaa
    24601 tctctacacc aaaatcaccg gctctcagcg cctggcgatg tttggcgcac agaaagacga
    24661 tctgccagag atctggcgtc aactgattga agccggcttc gaaaccggtc atgcctatgc
    24721 gaaagcactg cgtatggcga aaacctgcgt gggaagcacc tggtgccgct acggtgttgg
    24781 cgacagcgtc ggcctcggcg tggaactgga aaaccgttac aaaggcatcc gtacgccgca
    24841 caaaatgaag ttcggtgtct ccggctgtac ccgtgaatgt tcagaagctc agggtaaaga
    24901 cgtgggtatt atcgccaccg aaaaaggctg gaacctgtac gtttgcggta acggcggcat
    24961 gaaaccgcgt catgcggatc tgctggcagc ggatatcgat cgcgaaacgc tgatcaaata
    25021 tcttgaccgc ttcatgatgt tctacatccg tactgccgac aaactgacgc gtaccgcgcc
    25081 gtggttagaa aacctcgaag gtggcatcga ttacctgaaa gcggtgatca ttgacgacaa
    25141 actggggctg aacgcacatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg
    25201 tgagtggact gaaacggtca ataccccgtc tgcgcagact cgcttcaaac acttcatcaa
    25261 cagcgataag cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc
    25321 aacgccgtat gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa
    25381 gacatctgca aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggtgac
    25441 gaacaagtag caattttccg cccgtatcac agcgatcagg tgtttgcgat cagcaacatc
    25501 gacccgttct tcgagtccag cgtgctgtca cgcgggttga ttgcggaaca ccagggcgag
    25561 ctgtgggtcg ccagtccgct gaaaaagcag cgttttcgct taagcgacgg cttgtgcatg
    25621 gaagacgaac agttttccgt caagcattac gacgcgcgag tgaaagacgg cgtggtacag
    25681 ctgcgcggtt aatattttaa cgggaggcgc aatgccttcc ctttttgcat ggtcctgtaa
    25741 taaccttcgg tat
//
LOCUS       DAZFRG010000044        25378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFRG010000044 DAZFRG010000000
VERSION     DAZFRG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.044"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHKUC_001437"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHKUC_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO6617720.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHKUC_001438"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHKUC_001438"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO6617721.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHKUC_001439"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHKUC_001439"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO6617722.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLTRQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHKUC_001440"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHKUC_001440"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO6617723.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAIYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGIDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHKUC_001441"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHKUC_001441"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO6617724.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6615..7109
                     /gene="lrp"
                     /locus_tag="ACHKUC_001442"
     CDS             6615..7109
                     /gene="lrp"
                     /locus_tag="ACHKUC_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO6617725.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7244..11233
                     /gene="ftsK"
                     /locus_tag="ACHKUC_001443"
     CDS             7244..11233
                     /gene="ftsK"
                     /locus_tag="ACHKUC_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO6617726.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPHQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQQQYQQPQQPV
                     AQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGVGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            11392..12003
                     /gene="lolA"
                     /locus_tag="ACHKUC_001444"
     CDS             11392..12003
                     /gene="lolA"
                     /locus_tag="ACHKUC_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO6617727.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12014..13357
                     /gene="rarA"
                     /locus_tag="ACHKUC_001445"
                     /gene_synonym="mgsA"
     CDS             12014..13357
                     /gene="rarA"
                     /locus_tag="ACHKUC_001445"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO6617728.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQALEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13448..14740
                     /gene="serS"
                     /locus_tag="ACHKUC_001446"
     CDS             13448..14740
                     /gene="serS"
                     /locus_tag="ACHKUC_001446"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO6617729.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            14979..17423
                     /gene="dmsA"
                     /locus_tag="ACHKUC_001447"
     CDS             14979..17423
                     /gene="dmsA"
                     /locus_tag="ACHKUC_001447"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO6617730.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYGIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGIPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILLDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKLRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17434..18051
                     /gene="dmsB"
                     /locus_tag="ACHKUC_001448"
     CDS             17434..18051
                     /gene="dmsB"
                     /locus_tag="ACHKUC_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO6617731.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18053..18916
                     /gene="dmsC"
                     /locus_tag="ACHKUC_001449"
     CDS             18053..18916
                     /gene="dmsC"
                     /locus_tag="ACHKUC_001449"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO6617732.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRTESQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(18952..19578)
                     /gene="ycaC"
                     /locus_tag="ACHKUC_001450"
                     /pseudo
     CDS             complement(18952..19578)
                     /gene="ycaC"
                     /locus_tag="ACHKUC_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
     gene            19892..21040
                     /gene="ycaD"
                     /locus_tag="ACHKUC_001451"
     CDS             19892..21040
                     /gene="ycaD"
                     /locus_tag="ACHKUC_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6617733.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLAPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(21137..21877)
                     /gene="pflA"
                     /locus_tag="ACHKUC_001452"
     CDS             complement(21137..21877)
                     /gene="pflA"
                     /locus_tag="ACHKUC_001452"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO6617734.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(22069..24351)
                     /gene="pflB"
                     /locus_tag="ACHKUC_001453"
     CDS             complement(22069..24351)
                     /gene="pflB"
                     /locus_tag="ACHKUC_001453"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6617735.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(24406..25263)
                     /gene="focA"
                     /locus_tag="ACHKUC_001454"
     CDS             complement(24406..25263)
                     /gene="focA"
                     /locus_tag="ACHKUC_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO6617736.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6286 a         6315 c         6674 g         6103 t
ORIGIN      
        1 ttgttgcctg acctcatggg gggcaacggg gcgctactat agggagttgg aataaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttctaaagt actccataca tatcacaaaa
      181 taaaaaggcc ggctaaaccg accttttact cattctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgaggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagtttacc gccatgacgg ataaattcat cacagaagac cagaagcgcc gttttggacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag tgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcgatg acctgaccaa aagcgtaatt catcgtgaca cgatagggtg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc cataacaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgccagg agaaaaccac gggaaaatac
     1321 cacgctgata agccattaac aggcgcgcag ggctaagatc gccccccagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggtaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc gcacatcatt tctgcaagca attcaaggat
     1681 ctgactttcg gttgtggcat ctaagccttc tgtaggttca tccagcaata ccagcggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgcca gagcctcatc actactacca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgggga acaacgctaa tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg actatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagataa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagcgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccggacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtgag ttcagattga cggcgttgcg cttccagcca
     2521 ctgaatttct gtattctcta attgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagt ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcacc taccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagt aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtatatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gctaccgctg aggcggagag gaaccagccg gaaagggtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc cgccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactgatg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcaccaaca ggcgtatcaa tgccttgcgg caggagcggt agaaactcac tgacccaggc
     3601 gttatccagc gctgtttgta actcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattcc ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacactgct cgttggcctg ctggcaaagt aaaattcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ttcaatggtc accggatcgg tcgatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcaaactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggctag aaaacccgca gccagcgtca caccagtatc
     4141 gtagtgacca aaatccagct cgccgaggta ggaaaaacca aagtagatcg ccaccagagc
     4201 aatcgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaat aacggaatta gcggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atagccacca caatcagcag cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagcacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaacagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaat tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgacttg
     5101 taaaattatt ttgcgtcagc taaaccgtcg aggtagcgtt ccgcatcaag agctgccatg
     5161 cagcctgtac cagcagatgt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatctga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa taatgttgcc gttctccact ttatccatca gacgcttaat gaggattttt
     5521 tccgcacgga aaccatcacg gcggtgaatc agatgaactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgc tatcgccagt cagacggaac ggacgattct gcaaatcaac tttgttgatg
     5821 tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg gcaggattgt agggaattta
     6121 cagacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg cagcatcgat
     6181 aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc gctttataag
     6241 ccgattaaat gatgaataaa cgcccctgtt aatgaatatc tggcatgttg tactaaaaat
     6301 cgatgttttg ctttgacaat cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa
     6361 cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa
     6421 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
     6481 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
     6541 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
     6601 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
     6661 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
     6721 aacgtgtggg actttcccca acgccatgcc ttgagcgtgt gcgtcggctg gaaagacaag
     6781 ggtttattca gggctatacg gcgctgctga acccccatta tctggatgca tcacttctgg
     6841 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
     6901 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atccggtgat ttcgactacc
     6961 tgttgaaaac acgcgtgccg gatatgtctg cttaccgtaa gttactgggg gaaaccctgc
     7021 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
     7081 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
     7141 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
     7201 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
     7261 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgccgtcttc tggaggcgtt
     7321 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
     7381 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
     7441 gcccggtgcg tggttggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
     7501 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata
     7561 cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc
     7621 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
     7681 tggcagctta ctaagcacta cgctacaacc actgctacac agtagcgggg gaactattgc
     7741 gctgctctgc gtttgggcag cgggcctgac gctgttcacc ggttggtcat gggtgaccat
     7801 tgctgaaaaa ctcggcggct ggattttaaa tattcttacc ttcgccagta atcgtacccg
     7861 tcgcgatgat acctgggtcg atgaagatga gtatgaagac gacgaagagt atgaagatga
     7921 aaatcacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg
     7981 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag atgctgcgct
     8041 gttctccggt aagcggatgg atgatgaaga agagattacc tacactgcac gcggtgtggc
     8101 tgctgaccct gacgacgtcc tgttttcggg caatcgtgca acgcagccag aatatgacga
     8161 atacgatcca ttattaaacg gtgcgccaat taccgaacct gttgctgtag cagctgctgc
     8221 taccacggcg acacaaagct gggctgcacc ggttgaacct gtgactcaga cgccgccagt
     8281 tgcctctgtt gatgttccac ctacgcaacc tacagtagcc tggcagcctg taccggggcc
     8341 gcaaacggga gaaccggtta ttgcacctgc accggaaggt tacccacacc agtcacaata
     8401 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa cctgtacagc cacagcagcc
     8461 gtattatgcg cctgcggctg aacaacctgt gcaacaacct tactacgctc ctgcggcgga
     8521 acaacctgtt cagcaaccgt attacgcccc tgcgccagaa caaccggtgg caggtaacgc
     8581 ctggcaagcc gaagagcagc aatccacttt tgctccacag tctacatacc agacggagca
     8641 aacttatcag cagccagctg ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca
     8701 gcagcctgtt gtggagcctg aacccgttgt agaagagaca aaacccacgc gtccgccgct
     8761 ttactatttt gaagaagtgg aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg
     8821 gtatcaaccg attccagaac cggttaaaga accggaaccg atcaaatctt cgctgaaagc
     8881 accttctgtt gcagcagtac ctccagtaga agccgctgcc gctgtttctc cgctggcatc
     8941 tggtgtgaaa aaagcgacac tggcgacggg ggctgccgca accgttgccg cgccagtctt
     9001 cagtctggca aatagcggtg gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt
     9061 gccacgaccg aaacgcatcc gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa
     9121 gctgccctca cagcgtgcgg cagaagaaaa agcccgcgaa gcccagcgta atcagtacga
     9181 ttctggcgat cagtacaacg atgatgaaat cgatgcgatg cagcaggatg aactggcacg
     9241 tcagttcgcc cagacgcagc agcaacgcta tggcgaacag tatcaacatg atgtgcccgt
     9301 gaacacagaa gatgcagatg ctgcggcaga ggcagaactg gctcgtcagt ttgctcaaac
     9361 tcaacaacaa cgttattccg gcgaacaacc ggctggggcg aatccgttct cgctggatga
     9421 ttttgaattt tcgccaatga aagcgttgct ggatgatggt ccacacgaac cgctgtttac
     9481 gccaattgtt gaacctgtac agcagccgca acaaccggta gcaccgcagc agcaatatca
     9541 gcagccgcaa caaccggtag cacagcagcc gcaatatcag cagccgcaac aaccggttgc
     9601 accgcagcag caatatcagc agccgcaaca gccggttgca cagcagccgc aatatcagca
     9661 gccgcaacag ccagttgcgc cgcagccgca tgataccctg cttcatccgc tgttgatgcg
     9721 caatggtgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
     9781 gacacctccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg
     9841 cctggtagaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
     9901 ggggccagtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cagcgcgcat
     9961 ttctaacttg tcacgtgacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    10021 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    10081 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    10141 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    10201 cttgttggtt gcggggacta ccggttccgg taagtctgtc ggggtgaacg cgatgatcct
    10261 gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat
    10321 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttaccgatat
    10381 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    10441 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    10501 taggatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    10561 gcatccggtg ctgaaaaaag aaccgtacat tgtggtgttg gtggacgaat ttgccgacct
    10621 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    10681 tgccgcgggt atccacctcg tactggcaac gcagcgtcca tcggttgatg ttattactgg
    10741 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    10801 acgtaccatt cttgatcagg ctggtgcgga atcactgctg ggtatggggg atatgctcta
    10861 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    10921 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    10981 cacctccgac agtgaaagcg aaggtggcgt gggcggtttc gatggcgctg aagaactgga
    11041 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    11101 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    11161 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc
    11221 gccatttgac taattaatgc aacgtagacc ggataagacg cgatagcgtc gcatccggta
    11281 ttctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt agcctggaat
    11341 agagagtaga gggaactccc gattgggagt gacgtaattt gaggaataat gatgaaaaaa
    11401 attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc
    11461 gcaagcgatc ttaaaagccg cctggataaa gtcagcagct tccacgccag cttcacacag
    11521 aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct gtgggtgaaa
    11581 cgtccaaact tattcaactg gcatatgact caacctgatg aaagcattct ggtttctgac
    11641 ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac ctggctgaaa
    11701 gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag cgactggcag
    11761 cagtacaata tcaaacagaa tggcgatgac tttgttctga cgccgaaagc cagcaatggc
    11821 aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca gtttagcgcg
    11881 gtggagcagg acgatcagcg cagcagttat cagctgaaat cccagcaaaa tggggctgtg
    11941 gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga tcaacgtaag
    12001 tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt ttcaacctct
    12061 ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac atttgctggc
    12121 tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta tgatcctctg
    12181 ggggccgccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct atgcgaacgc
    12241 tgatgtggaa cgtatttctg ccgtcacctc tggtgtgaaa gagattcgcg aggcgatcga
    12301 gcgcgctcgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg acgaagttca
    12361 ccgtttcaac aaaagccagc aggatgcatt tctgccacat attgaagacg gcaccatcac
    12421 ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac tgctttcccg
    12481 tgcccgtgtc tatctgttga aatccctgag tacagaggat attgagcaag tactaactca
    12541 ggcgctggaa gacaaaaccc gtggctatgg tggtcaggat attgttctgc cagatgaaac
    12601 acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga
    12661 aatgatggcg gatatggccg aagtcgatga tagcggtaag cgggtcctga agcctgaatt
    12721 actgaccgaa atcgccggtg aacgtagcgc ccgctttgat aacaaaggcg atcgctttta
    12781 cgatctgatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg cggcgctcta
    12841 ctggtatgcg cgaattatta ccgccggtgg cgatccgtta tatgtcgcgc gtcgttgcct
    12901 ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg tggcaattgc
    12961 ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc
    13021 gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt ttaaagccgc
    13081 gctggccgat gctcgcgaac gtccggatta tgacgtgccg gttcatttgc gtaatgcgcc
    13141 gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc atgatgaagc
    13201 aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa cacgctatta
    13261 tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct ggctggctga
    13321 acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt
    13381 taatgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg ataagcacag
    13441 gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg ctgaaaaact
    13501 ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgagg agcgtcgtaa
    13561 agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat cgaaatccat
    13621 tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag tgaacaaact
    13681 gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg aaattcgcga
    13741 tatcgcgcta accatcccta acctgcctgc agatgaagtg ccggtaggta aagacgaaaa
    13801 tgacaacgtt gaagtcagtc gctggggcac cccgcgtgag tttgactttg aagttcgtga
    13861 ccatgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagccgcag ttaagttgac
    13921 tggttcccgc tttgtcgtca tgaaagggca gattgctcgc atgcaccgcg cactgtcgca
    13981 gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg ttccgtacct
    14041 ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg gcgatctgtt
    14101 ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga tcccaacggc
    14161 agaagttccg ctgaccaacc tggtacgcgg tgaaatcatc gatgaagatg atctgccaat
    14221 taagatgacc gcccacaccc catgtttccg ttctgaagct ggttcatatg gtcgtgacac
    14281 tcgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga tcgtgcgccc
    14341 ggaagactca atggcggcgc tggaagagat gaccggtcat gcggaaaaag tcctgcagct
    14401 gctgggcctg ccgtaccgta aaatcatcct ttgtaccggc gacatgggct ttggcgcttg
    14461 caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgcg agatctcttc
    14521 ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc gtagcaagtc
    14581 ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtcttgctg ttggtcgtac
    14641 gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag taccagaagt
    14701 tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt ttctgaatct
    14761 aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata attactcctc
    14821 acttacaagt aatactactt tcgagtgaaa atctacctat ctctttgatt ttcaaattat
    14881 tcgatgtata caagcctata tagcgatctg ctatagaaat aattacacaa tacggtttgt
    14941 tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa atccctgatg
    15001 cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc
    15061 tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata
    15121 gcgctattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg
    15181 gtagtcgctg cccgctacgt atgcacgtag tggacggtga aatcaaatat gtcgaaacgg
    15241 acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgt ctgcgtgggc
    15301 gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg aaacgagtcg
    15361 gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcttac ggcatcatcg
    15421 cgaccaatat gcagcgcttg atcaaagagt acggcaacga atccatctat ctgaactatg
    15481 gcaccggtac gctgggtggc accatgaccc gctcgtggcc gccgggaaat accctggtcg
    15541 cgcgcctgat gaactgctgc ggcggttatc tgaaccatta tggcgactac tcctccgcgc
    15601 aaattgcgga aggtctgaac tatacctacg gcggctgggc agatggtaac agcccgtcgg
    15661 atatcgaaaa cagtaaactg gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga
    15721 gtggcggtgg ggtgacttac tatcttgaac aggcgcgcca gaaatctaat gctcgcatga
    15781 tcatcatcga tccgcgctat accgacaccg gtgccgggcg tgaagatgag tggatcccta
    15841 ttcgtccggg aacagatgcc gcactggtta acggtctggc gtatgtgatg atcaccgaaa
    15901 acctggtaga tcaggaattc ctcgataaat attgcgttgg ctacgacgag aaaaccctgc
    15961 cagccagtgc gccgaaaaat ggtcactata aagcttatat tctgggtgaa ggacccgatg
    16021 gcgtggctaa aacaccggaa tgggcttcgc aaattaccgg tatcccggca gataaaatca
    16081 tcaaacttgc tcgtgaaatt ggtagcacca aaccggcatt tatcagccag gggtgggggc
    16141 cgcagcgcca tgccaacggc gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga
    16201 ccggtaacgt tggtattaac ggggggaaca gcggcgcgcg cgaaggttca tacagcttac
    16261 cgtttgtccg tatgccgact ctcgaaaacc cgattcagac cagcatttcc atgtttatgt
    16321 ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga
    16381 aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta
    16441 atcagcattc tgaaatcaac cgtacccatg aaatcctcct cgatgataag aagtgcgagc
    16501 tgattgtggt tatcgactgt cacatgacct catcggcgaa atatgctgac atcttgctgc
    16561 ctgactgcac tgcttccgaa cagatggact ttgcactgga tgcatcctgc gggaatatgt
    16621 cttacgttat tttcaatgat caggtcatta aactgcgctt tgaatgtaag actatctatg
    16681 agatgaccag cgaactggca aaacgtcttg gcgtcgaaca acagtttact gaaggtcgta
    16741 ctcaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc
    16801 caacgtttga agagttccgt aagcagggga tctttaaaaa gcgcgatccg caagggcatc
    16861 acgttgctta caaagccttc cgtgaagatc cgcaggctaa tccattgacc acgccatcgg
    16921 gcaaaattga gatctattca caggcgctgg ctgacattgc cgctacctgg gaattgccag
    16981 aaggtgatgt gatcgatcca ctgccgatct atacgccggg ctttgaaagt tatcaggatc
    17041 cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct cgcgttcact
    17101 caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg tggatcaacc
    17161 cgcttgatgc gcaaaaacgc ggtatccaca acggcgataa agtcaggatc tttaacgatc
    17221 gtggtgaggt ccatattgag gcgaaagtga cgccacgaat gatgccgggt gtggtcgcac
    17281 tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggc ggttgtatta
    17341 acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc
    17401 ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt
    17461 gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat
    17521 ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggtggcga ctggcaggaa
    17581 gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc atgtaaccac
    17641 tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgtgaagat
    17701 ggttttgttg tggtcgatga agatgtatgc attggctgcc gttactgtca tatggcttgc
    17761 ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt
    17821 tatgaccgtg ttgcggaggg aaaaaaaccg atctgcgtag agtcctgccc gctgcgggcg
    17881 ctggatttcg gccctatcga cgagctgcgt aaaaaacacg gcgatctggc ggctgttgcg
    17941 ccgttgccgc gtgcgcactt caccaaaccg aatattgtga tcaaacctaa cgccaatagc
    18001 cgcccaaccg gggataccac tggctatctg gcaaacccga aggaggtgtg agatgggaag
    18061 tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg tagcgggtgg
    18121 ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgaacagaat cccagcagcg
    18181 tgttatcgcc tgcatgtttg gtttatgggt gctgatgggc attggcttta tcgcctctat
    18241 gcttcatctt ggttcaccga tgcgcgcttt taactcgctc aatcgggtag gggcttcagc
    18301 actcagtaac gaaatcgcca gcggttcgat cttttttgct gtaggcggca tcggctggtt
    18361 gctggcaatg ctgaaaaagc tttcaccggc attgcgtacg ctttggctgg tggtgacgat
    18421 ggttcttggc gtagtctttg tctggatgat ggtgcgtgtg tataacagca tcgatacagt
    18481 acccacctgg tacagcatct ggacaccgat gggcttcttc ctgacgatgt ttatgggcgg
    18541 cccgttactg ggttacctgt tgctgagtct ggctggtgtc gatggctggg cgatgcgtct
    18601 gctgccagcc atttctgttc tggcactggt agtgagtggc gtgatgtcag tgatgcaggg
    18661 cgcagagctg gcgactattc atagctctgt gcagcaggcc gcagcgctgg tgccggacta
    18721 tggtgcgctg atgtcctggc ggattgtgct tttggccgtt gccctgtgct tgtggattgc
    18781 accacagcta aaaggttatc agcctgcggt tccgctactt tctgtatcat tcattctgct
    18841 tctggcaggg gaattaatcg gtcgtggcgt attctatggt ttgcatatga ccgtggggat
    18901 ggctgtcgca agttaatcag taacaaccgg ggtttcggcc ccggttcgct tttatttctg
    18961 cttcgttaac gtgtcataac tggtcatcaa gttacgataa tcaggaatgt ggttagagaa
    19021 caacgtcgcc agtccttcaa tatcattacg ccagtcgcga tgcagctcgc atgccacgcc
    19081 gaaccatgtc atcaattgcg caccagcttg cgacatgcga tcctatgccg aatgccgggt
    19141 aatttcatta aaggtccctg acgcgtcagt cacgacaaag acgtcaaacc cttcttcaat
    19201 ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa taattaactg
    19261 ttttttacct gtcgctttga cagcttttac aaaatcttcg ttatcccagg cgttaatatt
    19321 tcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg gaactaatgg
    19381 gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga aatacttagc
    19441 caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat cccgtacaag
    19501 ggaaagtaaa ccagcctggt gatcaacaag caaaacggca gcatcatttt tatcaagacg
    19561 aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgtc taaatcggtc
    19621 agacgtgatg cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa
    19681 atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga
    19741 gattaatcgt ctcttcagca ttagcgttat gaatgagtaa tataaatcga taataccgcc
    19801 gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca
    19861 tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct
    19921 tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata cactcgcgcc
    19981 gctttggctc gcccaggaac acatgtctac atggcaggta ggcgttgtca gctcatccta
    20041 ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac gcattggctt
    20101 taaccgtagc tattatctgg cctccttcat ttttgccgct ggctgtgccg gccttggcct
    20161 gatgattgga ttctggagtt ggttggcgtg gcgttttgtc gctggcatcg gctgtgccat
    20221 gatttgggta gttgttgaga gtgcgctgat gtgcagtggg acgtcgcgta accgcgggcg
    20281 gttgcttgct gcgtatatga tggtttatta cgtcggaacg tttttaggcc agttactggt
    20341 cagcaaagtc tcaaccgagt tgatgagtgt attgccgtgg gttacaggtt tgacgttggc
    20401 aggaatctta ccgctgttgt ttacgcgtgt cctgaatcag cagactgaaa accatgattc
    20461 gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat
    20521 tatctcagga atcgtgctgg gatctctata tggcctgatg ccgctgtacc tcaatcacaa
    20581 aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg cgggtatcct
    20641 cgggcaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg tgttgcgtgt
    20701 tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg cgatggcccc
    20761 agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc
    20821 ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac tgttgagcta
    20881 taccgtggga agcctgcttg gcccgtcatt taccgctatg ctaatgcaga atttctccga
    20941 taatttatta tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg
    21001 taacgccggt catacgccga aacccgtggc tcacgtgtaa atgaattcaa gcagagtgtg
    21061 aacttactgt ttcacactct gcttttctgt gcacaaaaaa gcggtaactc aataaagttg
    21121 ccgctttacg gggaaattag aacatgacct tatgaccgta ctgctcaaga atgcctttca
    21181 cgcgttccat ggtctctttc ttcggtggtt taacaccatc gagtttgtat tcttcaccca
    21241 tcgccaccca tttgtgtttg cccagttcgt ggtaggggag aagttcgatt ttctcaacgt
    21301 tacccatatc acgggtaaat tcaccgaggc gatgcgctga atcgtcatcg tcagaccagc
    21361 cagggacaac aacatagcgg atccacacct tcacattttt gttcgccaga tatttagcga
    21421 actccagcgt gcggtggttg gaaactccaa ccagattttg gtggatctcg tcgttcatct
    21481 gtttgagatc gagcattacc aggtcggtta cttccagcag ttcatcaatc accggatcgt
    21541 aacgacgaac aaaaccgttg gtgtccagac aggtatgaat gccttctttt ttgcaggcgc
    21601 ggaaccagtc acgaacaaac tcagcttgta ggattgcctc accgccggat gcggtaacgc
    21661 cgccgccgga agcgttcata aagtggcgat aggtcaccac ctccttcatt aaatcttcaa
    21721 cggtaacttc tttaccgcca tgcgtgtccc aggtgtcgcg gttatgacaa tacaggcagc
    21781 gcatcaggca gccctggaaa aaggtgataa agcgaatacc cgggccgtct acggttccac
    21841 aggattcaaa ggagtgaatg cgaccaataa ctgacattgc ggtgtttctc cagatgtggc
    21901 ccatctgagg ccgtgttggt gcgcagctcg aaggctacgt cgagtctgtt ttggcagtca
    21961 ccttaaagta tagatagctg acaaaaaagg ctctcgtgct aaaaaaggcc ccactttcgt
    22021 ggagccttta ttgtacgctt tttactgtac gatttcagtc aaatctaatt acatagattg
    22081 agtgaaggta cgagtaataa cgtcctgctg ctgttcttta gtcagcgagt tgaaacgtac
    22141 tgcgtagcca gatacacgga tggtcagctg cggatatttt tccgggtttt ccatcgcgtc
    22201 gagcagcatt tcacggttca tcacgttaac gttcaggtgc tgaccacctt cgatggatgc
    22261 ttcgtggtgg aagtaaccat ccatcagacc agccaggttg gtcttacgaa cttcgtcgtc
    22321 tttacccagt gcgttcggaa cgatagagaa ggtgtaggag ataccatctt tagcgtaagc
    22381 aaacggcagt ttagcaacgg aagtcagaga cgctacagca cctttctggt cacgaccgtg
    22441 catcgggtta gcacccggtc cgaacggcgc gccagcacga cgaccgtctg gggtgttacc
    22501 agttttctta ccatacacaa cgttagaagt gatggtcaga acagactgag tcgggatagc
    22561 gtcacggtag gtgtgcagtt tctgaatttt cttcatgaaa cgttctacca ggtcaacagc
    22621 caggtcatct acacgcggat cgttgttacc aaactgcggg tattcgcctt cgatttcgaa
    22681 gtcgatagcc agaccgtctt catcacgaat cggtttaact ttcgcatatt tgattgcaga
    22741 cagggagtca gcagcaacgg acagaccagc gataccacac gccatggtgc ggataacgtc
    22801 acggtcgtgc agcgccatca gagaggcttc gtagctgtac ttgtcgtgca tgtagtggat
    22861 gatgttcagt gcagtgatgt actgtttagc cagccagtcc atgaagtgat ccatgcgctc
    22921 catcacttca tcatagttca ggacatcgcc tttgatcggt tcagacttcg gaccaacctg
    22981 cattttcagt ttttcgtcaa cgccgccgtt gattgcgtac agcatggttt tcgccaggtt
    23041 tgcacgcgca ccgaagaact gcatttgttt accaacgatc atcgggctta cgcagcaagc
    23101 aatagcgtag tcatcgttgt tgaagtccgg acgcatcagg tcatcgttct catactgcag
    23161 agaagaggtg tcgatggaca ctttagcggc gaatttcttg aagttcagcg gcagtttttc
    23221 agaccacaga atggtcatgt tcggttccgg agacggaccc atggtgtaca gggtgttcag
    23281 gaaacggaag ctgtttttgg taaccagggt acgaccgtcg aggcccatac caccgataga
    23341 ttcggttgcc cagatcgggt cgccagagaa cagttcatcg tattccggag tacgcaggaa
    23401 gcgaaccata cgcagtttca tgaccaggtg gtcaaccatt tcctgcgctt cttgttcggt
    23461 gatcttgcca gctttcaggt cacgttcgat gtacacatcc aggaaggtgg aggtacgacc
    23521 gaaggacatt gcagcaccgt tctgagactt aacagcagcc aggtagccga agtaagtcca
    23581 ctggatagct tcctgagcgt tggtagccgg accagagatg tcgtagccgt atttcgcagc
    23641 catttctttc atctgaccca gagcgcggtg ctgttcagcg atttcttcgc gcagacggat
    23701 agtctgttcc aggtttacgc cgttttccag atcagcctgc agagaggtga actgtgcaag
    23761 tttgtctttc atcaggtagt cgataccgta cagcgcaacg cgacggtagt caccgatgat
    23821 acgaccacgg ccataagcat ctggcagacc ggtcagaaca ccggatttac ggcaacgcag
    23881 gatgtccgga gtgtaaacgt cgaacacgcc ctggttgtga gttttacggt attcagtgaa
    23941 gatttttttg atcatcgggt ccagttcgcg gttgtacgct ttgcaggaac cttcgatcat
    24001 tttgatacca ccgaacggga taagagcacg tttcagcgga gcttcagtct gcagaccaac
    24061 gattttctca agctgcttgt tgatgtaacc agcgtcgtga gaggtgatgg tggaagcaac
    24121 agcggtgtca aagtcaactg gcgcgtgagt gcggttttcc agtttaacgc cttccattac
    24181 tttgtcccac agggtggtgg tcgcttcagt agcgccagcc aggaaggact cgtcaccctc
    24241 gtacggagtg tagtttttct gaatgaagtc acggacgttt acttcattct gccagtcacc
    24301 tttggtaaaa ccttcccagg ctgtggctaa cttttcatta agctcggaca tgtaacacct
    24361 accttcttaa gtggattttt tatttactgc gtacttcgac aaccattaat ggtgatcgtt
    24421 ttcacgcagg taaatgaccc agtatgtcaa cccaaccaac aaaccaccgc cgataatatt
    24481 accgatcgta accggaatca ggttatcagt gatgaaattc atcacggtca ggtgagaaaa
    24541 attttccggt gcagaaccga cagcggtcca gaattccgga gatgcgaagt cgcggattac
    24601 aatacccatc gggatcataa acatgtttgc gatactgtgc tcaaaaccgc tggcaacaaa
    24661 catcgcgacc ggcagcacca taataaacgc tttgtccatc aggctgcggc cagaataact
    24721 catccatact gccagacata ccatcaggtt cgccaggata ccaagacaga cggcctcaat
    24781 aaaagtatgg tgcactttgt ggtcggcggt ttgtaggacg tttagtcccc attgaccatt
    24841 tgcggtcata tactcgccgg aaagccacat taaaagtaca aacagcagtg cgccgaccag
    24901 gttgccaaaa taaacattta gccagttttt cgccaactga ccccaggtga tgcgcccact
    24961 cgccttggca acaacaatca acactgtgga agtaaagaga tcggctccgc agacaacaca
    25021 aagaatcaac cccagagaga agcaaatgcc gccaaccagt tttgccatgc cgaagggcat
    25081 tgtacctgtg ccagtggttg ctgtgatata gaagacgaat gcgattgaga tgaaaacacc
    25141 ggcggtaatc gccagataga aagtcttaag cggatgtttc gttgctttat agacacctgc
    25201 ctcttcggcc actttggcca ttgcagcagg aagtaaaaga tcaaaagggt tgtcagcttt
    25261 cacactaact ctctctttat taagtcggcg acgagatact aacaaagcat tatagatgag
    25321 aaattgatat agatcatatc tcgcctggct tataggcccg taactcgcat ggttttta
//
LOCUS       DAZFRG010000045        25332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFRG010000045 DAZFRG010000000
VERSION     DAZFRG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.045"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..1906)
                     /gene="gss"
                     /locus_tag="ACHKUC_001455"
     CDS             complement(47..1906)
                     /gene="gss"
                     /locus_tag="ACHKUC_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO6617737.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2111..2977
                     /gene="yghU"
                     /locus_tag="ACHKUC_001456"
     CDS             2111..2977
                     /gene="yghU"
                     /locus_tag="ACHKUC_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO6617738.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3047..3295)
                     /gene="hybG"
                     /locus_tag="ACHKUC_001457"
     CDS             complement(3047..3295)
                     /gene="hybG"
                     /locus_tag="ACHKUC_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO6617739.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3308..3649)
                     /gene="hypA"
                     /locus_tag="ACHKUC_001458"
     CDS             complement(3308..3649)
                     /gene="hypA"
                     /locus_tag="ACHKUC_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6617740.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3642..4130)
                     /gene="hybE"
                     /locus_tag="ACHKUC_001459"
     CDS             complement(3642..4130)
                     /gene="hybE"
                     /locus_tag="ACHKUC_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO6617741.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVANPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4123..4617)
                     /gene="hybD"
                     /locus_tag="ACHKUC_001460"
     CDS             complement(4123..4617)
                     /gene="hybD"
                     /locus_tag="ACHKUC_001460"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO6617742.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4617..6320)
                     /gene="hybC"
                     /locus_tag="ACHKUC_001461"
     CDS             complement(4617..6320)
                     /gene="hybC"
                     /locus_tag="ACHKUC_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO6617743.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6317..7495)
                     /gene="hybB"
                     /locus_tag="ACHKUC_001462"
     CDS             complement(6317..7495)
                     /gene="hybB"
                     /locus_tag="ACHKUC_001462"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO6617744.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7485..8471)
                     /gene="hybA"
                     /locus_tag="ACHKUC_001463"
     CDS             complement(7485..8471)
                     /gene="hybA"
                     /locus_tag="ACHKUC_001463"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO6617745.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINSPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8474..9592)
                     /gene="hybO"
                     /locus_tag="ACHKUC_001464"
     CDS             complement(8474..9592)
                     /gene="hybO"
                     /locus_tag="ACHKUC_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO6617746.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9781..10068)
                     /gene="yghW"
                     /locus_tag="ACHKUC_001465"
     CDS             complement(9781..10068)
                     /gene="yghW"
                     /locus_tag="ACHKUC_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO6617747.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10187..11074)
                     /gene="yghX"
                     /locus_tag="ACHKUC_001466"
     CDS             complement(10187..11074)
                     /gene="yghX"
                     /locus_tag="ACHKUC_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO6617748.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11231..12271
                     /gene="gpr"
                     /locus_tag="ACHKUC_001467"
     CDS             11231..12271
                     /gene="gpr"
                     /locus_tag="ACHKUC_001467"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO6617749.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12311..12805)
                     /gene="yqhA"
                     /locus_tag="ACHKUC_001468"
     CDS             complement(12311..12805)
                     /gene="yqhA"
                     /locus_tag="ACHKUC_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO6617750.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            12996..13880
                     /gene="yghA"
                     /locus_tag="ACHKUC_001469"
     CDS             12996..13880
                     /gene="yghA"
                     /locus_tag="ACHKUC_001469"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6617751.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDAKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13919..14344)
                     /gene="exbD"
                     /locus_tag="ACHKUC_001470"
     CDS             complement(13919..14344)
                     /gene="exbD"
                     /locus_tag="ACHKUC_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO6617752.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14351..15085)
                     /gene="exbB"
                     /locus_tag="ACHKUC_001471"
     CDS             complement(14351..15085)
                     /gene="exbB"
                     /locus_tag="ACHKUC_001471"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6617753.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15337..16524
                     /gene="metC"
                     /locus_tag="ACHKUC_001472"
     CDS             15337..16524
                     /gene="metC"
                     /locus_tag="ACHKUC_001472"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO6617754.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16664..17323
                     /gene="yghB"
                     /locus_tag="ACHKUC_001473"
     CDS             16664..17323
                     /gene="yghB"
                     /locus_tag="ACHKUC_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO6617755.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17363..18262)
                     /gene="yqhC"
                     /locus_tag="ACHKUC_001474"
     CDS             complement(17363..18262)
                     /gene="yqhC"
                     /locus_tag="ACHKUC_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO6617756.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18456..19619
                     /gene="yqhD"
                     /locus_tag="ACHKUC_001475"
     CDS             18456..19619
                     /gene="yqhD"
                     /locus_tag="ACHKUC_001475"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO6617757.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19724..20551
                     /gene="dkgA"
                     /locus_tag="ACHKUC_001476"
     CDS             19724..20551
                     /gene="dkgA"
                     /locus_tag="ACHKUC_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO6617758.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20751..21677
                     /gene="yqhG"
                     /locus_tag="ACHKUC_001477"
     CDS             20751..21677
                     /gene="yqhG"
                     /locus_tag="ACHKUC_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO6617759.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     PIERQFIYDNPICFGEESLFSRVDEIRVLGKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21728..21985
                     /gene="yqhH"
                     /locus_tag="ACHKUC_001478"
     CDS             21728..21985
                     /gene="yqhH"
                     /locus_tag="ACHKUC_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO6617760.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22027..24246)
                     /gene="ygiQ"
                     /locus_tag="ACHKUC_001479"
     CDS             complement(22027..24246)
                     /gene="ygiQ"
                     /locus_tag="ACHKUC_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO6617761.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     NELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24498..25247
                     /locus_tag="ACHKUC_001480"
     CDS             24498..25247
                     /locus_tag="ACHKUC_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6617762.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
BASE COUNT         6302 a         6735 c         6352 g         5943 t
ORIGIN      
        1 gtactgatta ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca
       61 caattaacgg ttcaatatca ctctcttttt tgatgaccag tgattcatca ccgcgcaaac
      121 acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca
      181 ctttcggcaa acaccacagt tgctggtaaa tatttttctg ttcggcaaat ttaccactgg
      241 tctgatcaag cacctcttca tgatggctga ccaggtcaat gttgctacca cagcgcccgg
      301 caatcggttt aacggcataa ccggttttca ccagctcatc attcacggtg aagtcagtat
      361 ccaacagata acgatggtgc gggaacagag accagaggat cggcagaatc gctttgttac
      421 cggggatcac cgtccatagc ggctcaaaaa ccaacacttc cgggcgcagc aatacgtcga
      481 taagacgcac ttcattttgt ggatgcccgg tacggatcgg cactgcggca aattcacggt
      541 cgctcacttc gcggatttga tcaaatgccg tttcccacgc ccaggttttc catacgcagt
      601 taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca
      661 acccacgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg
      721 cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac
      781 tgtgtttcca cgcgccagcc agttcgttaa tcagtccttc cgcaggattg aagccgttgc
      841 ctttatagcc ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacaggagg
      901 cagaatcggc gttgtattcg tagactttca ggccacgttc atccatgcag aaatccatac
      961 gaccagtgat catatgatga cggcgacgct gccaggagag acgcaaacgt ggccagagga
     1021 ttttcggaat gtcaaacagc gccagcaggt tgtcatcttt cagcaccttg tcggtggcgt
     1081 gaagatacat caggtgcagc tcgttggtgg ctttaatcag ctcctgctcg gcactctcgg
     1141 taatggtgta gtaatgatac ggatcctgat tgatcacctg gccgttggcc tgtacatagg
     1201 cgttttgcag cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca
     1261 gacgcgcccc gctgattttc agcagttcgc ctgcaatttc cggctgtggc aagctgtatt
     1321 cggtatcttc cgtctggatc atccagccca gaatagtggt gtcatcgaag gtgtctttca
     1381 gggtatagcc gccgttttcg accaccatct ccagctcgcg tgtccactgt tgcccttgcg
     1441 gcaacggggt atgaatcacg ttctgttccg caatacggac tttgttgcca tgcaattggg
     1501 taatgatggc gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg
     1561 cccccgctac cggtgcacgc ggcgaaccgt taggaaatgc ctgcaatggc aggatgttgt
     1621 cattgaccac ttcacgcagg aagcgcagtg agaaaatctc ccatgccata cccacgtcag
     1681 tgaagactac accgtagttc agaaagagaa aacggcgagc aaattcaacg cattgccatt
     1741 tgtggcccat atattcatcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct
     1801 gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca
     1861 ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa
     1921 caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta
     1981 accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa
     2041 aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag
     2101 gcaactcact atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc
     2161 cgctggcggc gcgttcgcca gtatcaatcg cccggtttct ggtccgacgc atgaaaaaac
     2221 gctgccggtg ggcaaacatc cattgcaact ttattcgctg ggaacgccga acggtcagaa
     2281 agtaacgatt atgcttgagg agctgctggc actgggcgtt actggtgcag agtacgacgc
     2341 ctggctgatt cgtattggcg atggcgatca attctccagc ggctttgtcg aagtgaaccc
     2401 aaactcgaag atcccggcgc tgcgcgacca tacccataat ccaccgatcc gcgtgtttga
     2461 gtctggatcg attctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggattt
     2521 ggcaaagcgt actgaaacga tgaactggct gttctggtta cagggcgcgg caccgttcct
     2581 cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt acgccatcaa
     2641 ccgctttacc atggaagcca agcgcctgct cgacgtgctg gataagcaac tggcgcagca
     2701 caagtttgtt gcgggcgatg agtacaccat tgcggatatg gcgatctggc cgtggtttgg
     2761 caacgtggtg ttaggcggcg tgtatgatgc cgcagagttt ctggatgcgg gcagttataa
     2821 gcacgtgcaa cgctgggcga aagaagtggg cgaacgtccg gcggtgaagc gtgggcgtat
     2881 tgttaaccgc accaacgggc cgctgaacga gcagttgcat gagcgccatg acgccagtga
     2941 tttcgagacg aatacggaag ataagcgtca ggggtaagtc gcaacattgg caggcttatc
     3001 gctatgtagc cggataaagc atttacgccg catccggctt taatcatcac gcgctggtaa
     3061 tgtcgtaatc catttggcgc agtgcgtcta atgtggcttt ggcttcatct tcgtcgatga
     3121 tgctcatggc aaatccgacg tgtaccagca cccactggcc cagcagatcg gcagggttac
     3181 cttcacaaat cagggcgata ttcacgtcgc gcttgatacc acatacttca acctgcgcaa
     3241 gctggtgaat atcttcaccg acagccagca cctggcctgg aacgccaata cacattgtta
     3301 actccagtta ttcaacttca atacttttga cgatcagcga atcgccggta tcgacacgca
     3361 accgctcgcc atgacagagc ggacactgcg catcgtgctg atgaatctcc accacctggc
     3421 tgcaatccca gcaccaggcc tgggcgggtt tatagacgat atgtaaatcg cacccttgcg
     3481 ccaccgttcc gtggcagaca atttcaaaac taaaacggac ggcgctctcc tcaacgcagg
     3541 agagcgcgcc aatttccagc cacacggcgg tgacgcgctt aacatcgtgc tgctccgcct
     3601 gccgttggat aatttcaacg gcgctctggc aaagcgacaa ctcatgcatt ttcgccactc
     3661 ctgcgaccaa acagcagggc gcgacgccct gcgtgtggta catccggatt agcgaccggt
     3721 agcgaaagga tcatccgcgc gcagtcatcc gtcaggcgtt ggccctcttc aatcgacatg
     3781 ctatgcgaaa gcggtgacat cagcgaacag gagagatatt gagaaacact gtccagttcg
     3841 ccaacggtaa aggtcatagt gccatacggc agttgcagac caattttttc actcactttg
     3901 cgcagcggcc agagttgatc cgggccgggg aagataactg cactcagcat ccacggggtg
     3961 atcacacacc ccgtccactg accttcgaac agcgtaaagt cagaaacata caccggcatt
     4021 gagggatgca gaaaagagag atcgtgcatc gaacgccggg caatttcttc aaacgctgcc
     4081 tgtacttgcg ccttcgggga ggtctggaaa cctgctatct cttcagtcat gagtcgcctc
     4141 ccgtgggata gcttccacgc ctgattcgcg cagcgcagcc agaacctgct caagcgcagg
     4201 ttcaatcatt gcttcaaccg tcggcgttaa gccgatgtgc ggctccagcg attccgggat
     4261 cacaccgacc agcgtcagct ttttcggaaa ctcgccggtg aagcgcaggg ccgacaatac
     4321 gtcggccagg ccaagctgat gcggagagat tttgttggta aacaacgccg gaacttcttc
     4381 atcccgcagg atcatcatcg ttcccggcgc gttctttttc gacacaatgg catccgcaat
     4441 aatcagatga tcgcgatttg ccatgtcgcc aagcagctcc attcccgccg tgccgccatc
     4501 gaggatctca acataatccg gcagaatgta tcgttgctct aacgcttcaa caatccgcac
     4561 accgatggct tcatcggtca gcaaaatatt gccgacccct aagactaaaa tacgcattac
     4621 agaaccttca ctgaaaccac ttcgttgccg tcggcatcca ctacgtgtac cgcacaggcc
     4681 atgcacgggt cgaaggagtg aatggtacgc accacttcca gcggtttatt cggatcggca
     4741 accggtgtac ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa gttacgcgga
     4801 ccagagttcc aggttgatgg aacaaccgcc tggtagttgc tgatgatacc gtctttaata
     4861 accatccagt gagagagcat accgcgcggc gcttcgagga agccaacacc tttgaactca
     4921 cccgttgccg gaatgttcgg cttcacaaag gtggtgtgat cgcctttgcc gatattggtg
     4981 atcagtgcac tgtattggtt ttgcaggata tcctgcaatt cacagcagtg aacggtacga
     5041 ccaataatac ggcccagcgt ggagtgcagc tgtgccactt ccagcgtgtt gccagtcagt
     5101 ttctgataaa tcgcaacgat ttcattcagt ttgttctggg tagattcgcg acctgccgcc
     5161 agtttcacca gcatattagc cagcggaccc acttctaccg ttttgccgta gaaagtcggc
     5221 gatttcaccc aggaatattt accgtcatca gaccaaccgt cgtaagccgg aatggtggta
     5281 ccttcccacg gtgcctgcgg cgcttcgtct ttataccagg agtgcttcgc gctttcctga
     5341 atgcctttga tcaggtattc atcggaatga gaagtgatcg gacgatacga ggacagatcc
     5401 gcattctcaa tgtagccgcc cgggaacagg aagctgccgt ttttgctgtc ggtcgggaat
     5461 tccggcacgc tcaggtagtt caccgcacct ttaccgcgcg tcagccattc cgggtagaac
     5521 gcggcgataa ctgcggtatc aaccttataa acctgttcaa caaagtcgct cagcttgtcg
     5581 atgaaagact tgatgtacat caggcgctca aggttcagca cgcccaaacc gtcgaggttg
     5641 attgggttag cgaccccgcc taccgccagg ttctgaatgt gcggcgtttt accgcccagc
     5701 agcgccacga cgcggttagc gtcacgctgg cactccagcg cctgcaggta gtgcgctacc
     5761 gcaatcaggt tcacttccgg cggcagtttc atcgccgggt gaccccagta gccattggcg
     5821 aaaataccca actgaccgct ggcaaccaga tctttaatct tgttctgaac tttggtgaac
     5881 tcttccggac tgttcagatg ccaggtcgaa acgcctttca gcatttcaga ggctttggtt
     5941 gggtcagctt gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag ctgatagaaa
     6001 tgaacgatat ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat gtattgcgcg
     6061 ttaaccggaa cgtcgatatt cagcgcactt tctgccgcac gaacggaaga cagcgcgtga
     6121 gtggtagtac atacgccaca gatacgttgc acaatcatcc atgcatcgcg cggatcgcgg
     6181 tttttcacga tctcttccat gccgcgccac atggtaccgg aagcccatgc tttcgaaacg
     6241 acgccatttt cgatttcgca atcgatgcgt aaatgcccct caatacgggt taccggatca
     6301 atagtaattc tctggctcat gctttgctcg cctcatgacg attatgatcg ttttgtttta
     6361 aaggaggaag tatcggcagt agacgaatga gtacgatgta agcgcaaatc tcaatagcca
     6421 caaaaccaat agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa ccgccgcccg
     6481 ggttgaatgc caccagcgaa taggtcagac gccaggttgc acaacctaac agtgcgctca
     6541 gtgctgacag gaacagcatg cgggaatcat tacgcagctt cgccacacgc agaacgacca
     6601 gcgggaagag catcagcagg acttcaatcc agaacatcac ggagtagaag tcaccggcaa
     6661 acgctaacga cagcttgtcg cgatagataa gctcgccaaa gcgcagcacg atgaaaatcg
     6721 ccagcaacac actgatggtg ttggtcagct tgacgaacag gctcttttca tccggaccgt
     6781 tgccacgcag acccgcctgc accagtgaac cttcaaagat gacaatcgag aagcccatga
     6841 tgaacgccgt cagcagcgag aacagcggca acatttcata gctctgccac agcggatgca
     6901 ccttgtagcc cgccgagatc atcagcgacc ccattgaaga ctggtgcatg gtcggcagca
     6961 gcgcaccgag cgcgatgatg aagaacatca ccttgttcag acgctgtagc gacaccttcc
     7021 agcccagacg ttcaaacagt gccggagcaa actccagtgc catcacgccg atatagatgg
     7081 tcatacagac cgccgtctcg aacagtaccg agttcacgtt gaagtgaccc ggaatgtaga
     7141 agtacggcag gttccagtag cgacccacgt cgatagtgat cgacaagcca cctagtgagt
     7201 aaccaaacag actcgccaac agcgccggac gcaccagcgg atggtattgc ccacggttaa
     7261 agacgtatac cgcccacgcc agcgcccagc cgccacaggc aaagccggtg ccgatcaaca
     7321 ggtcaaacgc gatccacacg ccccacggga agccgccgtt caggtcagag acagagccca
     7381 gaccgaacac cagacgcttc acaatcagga gcatacagat gacgattaac ggtccaaaaa
     7441 tcatgaccgg tttactgatg attttgccgc ccagcggttg tggatcatga ctcatgatcg
     7501 tctcctccgt cgtgatggtc gtttttggtg ttgcgacgaa ccagcacggt taagcccgcc
     7561 agcacagcca gtggtagcat catgccttta tacagggtgt gttgaatatt ttcggaacgc
     7621 gcaccggtag aaagatcgtc cagtttcggc aggtcgagat tttcataagg cacacccgtc
     7681 agtaccagaa cctgagtacc gccgccctct ttctcgccgt acagatgcgg ataatatttc
     7741 ggcaccgtat gcaggtaagt gtcgccagat ttcagcgtct gacgcggata gtggtattcg
     7801 ctgccaggct tcagcgccag acgttttttc gcctccgcca tcagctcttc acgcgtacca
     7861 aaaatcaccg cgcccgccgg gcacacttct acgcagccag gcagaccgcc tttatcgaga
     7921 cgttccacac ctttctggtt gcacaactcg cacttatgca gcgcaccaaa cgggttgttg
     7981 tagtcgtact tcggcacgtt gtatgggcag gcgaccatgc agtaacggca gccggtgcac
     8041 acatctttgt cgtaatggac aatgccggtt ttcggatctt ttttcagcgc agagaccggg
     8101 cacacagaga cacagttcgg atcgacgcag tgcatacact gtttcttaat gtacgcgtag
     8161 ccattctcct cctggtcttt gttgacccct gtgccgctgg tccacacctg aatgatgtta
     8221 ttggtatacg gcgacagttt gtcgttgttc gaccaggtct gttccccttg cgggttacgt
     8281 tcaggggagt tgatatcctg acacttggtg acgcaagcct gacagcctac gcacaaggtc
     8341 gagtcgtaca acatccccag cgatcccgga attggcgggc ggttttcagc agccgcatga
     8401 ctgacagacg gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt aataaaatta
     8461 cgtctgttca cggttattct ccccgtgagt cagcgttatc tttcttttgc tgacgaccca
     8521 gttcacgcac cgccatcacg ctgacaccgg caaccagccc aaccactccg ccgagcaaac
     8581 caatagcgcc tgccgagacg ttgccgcctt ctttagcgtt aacgtccggt ttctgtgaac
     8641 gcggagtctg gttttcgacg ttggcaagct gatggatgcc tttatggaag ccgatacctt
     8701 cttcgttaca gccatagcac gggtgaccaa tcgctaccgg ccacacgccg ccaacatcgc
     8761 agaattgcag cgttgagcag ttgccgtaag tttctggccc tttacagccg aggtggtaca
     8821 ggcaccagcc ttcgcggtgg ccttcatcac cgaactcttt ggcaaaacga ccagcatcga
     8881 agtgcgggcg acgttcacag tgttcgtgaa tcagacgacc ataggcgaaa gtcggacggt
     8941 ttttgtcatc cagtttcggc ggtttgccgt aagtgatgat gtgcgcaacg gtcgccagga
     9001 agttgtgcgg gttcggcggg cagccaggaa tattgataac ggttttgccc ggtagaactt
     9061 cttgcaggct gactgcgcca gttgggttaa ctccagctgc ggcaacaccg ccccacgcag
     9121 agcaggaacc gatagcgata atggctgctg cgccttccgc cgctttgcgg atgtgatcca
     9181 caatcggctc accggcaacc atgcaataaa taccgttatc ttttaatggg atggaaccat
     9241 ccaccactaa cacatactgc cctttgtact tctcgagagc gttatgtttg ttctcttcga
     9301 cctgatgacc gaaggcggcg gaaagcactt cgtgatactc cagagagata gtctccagca
     9361 cgaggttttc taccgttgga tgcgttgcac gaagcagaga ttccgtacaa ccggtgcact
     9421 cctgcgcgcc aatccagata actggcggac gctgcgggtt agtaaccgat tcgaccatct
     9481 ctgcagcggc tttgctactt aaccccatgg tggcggctaa tgctgcacaa agcttcatga
     9541 aatcacgacg gttaatgccg tgagaatgga tgagggtgtt atctccagtc atttatagtt
     9601 attccgttgc gaagacctgg catatatttt gcctcaatcg caaaatcaat aatgcgatcg
     9661 atgcgccatt taccacacat ttattatggt tatcaatatc atgatactgc ggcggggcac
     9721 taaaacgaag ggaagaagca ttagtgtaat taattaaagc aagataatac gtatgtttga
     9781 tcaattttct aggcgataac cggccataaa aaagcgcaac gtagaacagg aattattctc
     9841 acgaaagaaa tccattacca tctctttatc cagtccataa cggcgcgtca gaatacccgc
     9901 ttcccaggcg ctaagctggc gatctccggt cttttcgtac atccggtgtg agtagcctaa
     9961 tacaaaaccg cgtttataat cggcacagaa ttttgtcaca ttaaccgcac tgtcggcatg
    10021 cgttgctttt aatcccgcca ttaagccttt accaaaatgg ttattcatcg ttcacctcat
    10081 tcgctatata ttgtcatata tagcgatatt ttaggcaaag aacgacattt gcgcacgcta
    10141 ttttttcatg cttgtttaag acgtaatcct gtaagagata aacccatcag gagagatatt
    10201 tatcgaacca tttcagcgtc ctttgccagg caagatcggc ggcagatttg tcataacggg
    10261 gcgtggaatc attatggaat ccgtgattaa cccccggata tatatacgcc tcatagacct
    10321 tattattggc tttcaacgcc gcctcgtaag cgggccagcc ctcgttgatt cggctatcca
    10381 gttcagcgta gtggagcagt aaaggcgctt caatcttcgc cacatcggca gtgggtgcct
    10441 gacgcccata aaacggcacc gcgcaggcca gttccggata cgcgactgct gccgcgttcg
    10501 atacgccccc gccatagcaa aatccgctaa tacccacttt gcctgtcgct tgcggatagc
    10561 gttgcataaa ctcaattgcg gcaaagaaat cattcatcag tttggttgga tcaacctgct
    10621 gttgcagttc gcggccttta tcatcgttac caggatagcc acctactgag cttaaaccgt
    10681 ctggcgccag ggcgatgtac ccagccttcg ccactcgccg cgtcacatct tcgatatacg
    10741 gattcagccc tcgattctca tgcaccacta ccacggctgg cgttttgccg ctcatttttg
    10801 cgggcttcac cagataaccc cgtacctcgc cgtgaccatt tggcgaggga tacgtgatgt
    10861 actcagcaac aatttccggg tcggtaaact ctacctgagt cgccaacgca taatttggct
    10921 tgagcaaatc aaacagcgct aatgccgtca tcccgcccac cgcatacttc gccgcaaggt
    10981 tgaggaactc acgtttcgag attttcccgt gagcgtaata gtcgtagtaa tccagcaact
    11041 cttgtgggaa atctttggcg gttaaacgcg gcatcgttgc actcctcaac tggtgttttt
    11101 ttaagcaaag cataagcgcg tgtttttgcc cagtttttcg tcactctgtg agccagacga
    11161 cgggatacgc gctggcgaat cgctaaacta gaaacattgt ttcgaaattg aacgatggaa
    11221 aggagaggtc atggtctggt tagcgaatcc cgaacgttac gggcagatgc agtaccgcta
    11281 ttgcggaaaa agtggtttac gcctgcccgc gttatcgctc ggtttatggc acaatttcgg
    11341 tcacgttaac gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg atttaggcat
    11401 tacgcacttt gatttagcca acaactacgg gccgcctcca ggaagcgcag aagagaactt
    11461 tggtcgcctg ctgcgcgagg attttgccgc ttatcgcgat gaactgatta tctctaccaa
    11521 agccggatac gatatgtggc ccggcccata cggctctggc ggctcacgta aatacctgct
    11581 cgccagcctc gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata tcttttactc
    11641 tcatcgcgtc gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg ctcatgctgt
    11701 acaaagcggt aaggcgctgt atgtcgggat ctcctcttac tcgccagagc ggacgcaaaa
    11761 aatggtcgag ttgctgcgcg agtggaaaat tccgctgtta attcatcaac cttcgtacaa
    11821 tttactgaac cgctgggtgg ataaaagcgg cctgctggat accctgcaaa ataacggcgt
    11881 gggctgcatt gcctttactc cactggcaca gggattgctg accggaaaat atctcaacgg
    11941 tattccgcaa gattcacgga tgcatcgtga agggaataaa gttcgtggtc tgacgccgaa
    12001 aatgctcacc gaagccaacc tcaacagcct gcggttattg aatgaaatgg cacagcagcg
    12061 tggacaatca atggcgcaaa tggcgttaag ctggttgctg aaagatgatc gcgtgacgtc
    12121 ggtattgatt ggtgccagcc gcgcggagca acttgaggag aacgtgcagg cgctgaataa
    12181 tctgacattt agcaccgagg agctggcgca gattgatcag catatcgccg atggcgagct
    12241 gaatctgtgg caggcgtctt ccgataaatg acctgttaat aacgggcgcg agaaccgcgc
    12301 ccgaatcaga tcagtgatta tgacgagtca gtcggtcaag atagcccatc acaaatgcag
    12361 agagcacaaa cgtcagatgg ataatgacgt accacatcag tttgttatcc gggacatttt
    12421 tcgcatccat aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc gacgctgcta
    12481 ctttgttttt cagcgacgtt gcatccattt tccccagcca gttcagcttc tctttgttct
    12541 cggagatatc cagttgtgag acgaaattct cgtaaccgga aaacatcacc atcaccagca
    12601 aaccgccaac cagcgtcata tccaccagcg acagcaacac gaggatcaaa tctgattccg
    12661 ccatcgagaa gatattcggc agtacgtgaa taatctcctg gaagaacttc agcgccaggg
    12721 caactaacgc cagcgaaagg ccaaagtaca cgggggcaag cagccagcga gaagcataca
    12781 ttgcattttc aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg agcaacagta
    12841 tatcgcaatt aagtaaacag cacgcaacag caaatcaaca tatacgttaa acctttgtcg
    12901 cataattctg agggttaggg atcttcctgg ttcccttatc aacgcatcga gagcgtctac
    12961 ccttaaagga cgttgaacct gaagggagaa aaatgatatc tcatttaaaa gacccgacca
    13021 cgcagtatta cactggtgaa tatcccaaac agaaacaacc gacgccaggc atccaggcga
    13081 agatgacacc ggtaccggat tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag
    13141 atcgtaaagc actggtgaca gggggcgatt ccggaatcgg tcgcgctgcc gccattgctt
    13201 acgcgcgtga aggggctgac gtggcgatca gttatcttcc cgtggaagaa gaagacgctc
    13261 aggatgcgaa aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt
    13321 taagcgatga gaaattcgcc cgttcgctgg ttcacgaagc acacaaggcg ttaggcgggc
    13381 tggatattat ggcgctggta gccgggaaac aggttgccat tcctgatatt gcagacctca
    13441 ccagcgaaca gtttcaaaag acctttgcca ttaacgtttt cgcgttgttc tggctaaccc
    13501 aggaagcgat ccccctgcta ccgaaaggtg caagtatcat caccacttcc tcaatccagg
    13561 cataccagcc aagcccgcat ttactggact atgcggctac gaaggcggca attctgaact
    13621 atagccgtgg cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc
    13681 caggcccgat ctggacagca ctgcaaattt ctggcggaca aacgcaggat aagatcccgc
    13741 agtttggtca gaaaacgccg atgaagcgtg cggggcaacc ggcggagctg gcccctgtat
    13801 atgtttacct ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac ggtgtctgcg
    13861 gcggcgagca tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg catcattttt
    13921 atttcgcttt cgcagtttct tcgccgacca gacctatttt caggtaaccc gcctgatgca
    13981 gcgtatccat taccttcatc aacgtctcgt aatcgacggt tttatccgct cggaagaaga
    14041 tggtggtgtc tttcttgcct tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg
    14101 tgaccggatc gttaccaata aacatcgagt tgtctgcttt caccgacaga taaaccggtt
    14161 tttccggacg cggctgcggc gtactggtag aagcaggcaa attcaccttc acatctaccg
    14221 tcgctaacgg tgccgccacc ataaagataa tcagcaaaac caacatcacg tcgataaacg
    14281 gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag gttttcgtta agatgcatcg
    14341 ccatcggaca ttatcctgcg cgtaattttt gtgcgacacg aaccggatgt gcagcggcgc
    14401 tggcttccag atccaggtca cggctttgca gcaacaatac ctgcgctgca acatcaccca
    14461 gcatcgcttt aaagccgcca atctggcgtg caaatacgtt atagataacg accgcaggaa
    14521 tcgctgccac gaggccgatt gccgttgcta acagagcttc tgcaataccc ggcgcaacga
    14581 ctgccaggtt agtggtctgc gtttgcgcga taccaataaa gctgttcatg atgccccaga
    14641 ccgtaccaaa cagaccaacg aacggcgaaa tcgcgccgat ggttgccaga tagccgttac
    14701 cacggcccat ttgacgacct actgcggcga cccgacgctc cagacggaag ctggtacgtt
    14761 ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc cagctcattc tgcgcttcat
    14821 tgagcaaatg caggctcaag cttttgctac caaaatcagc ggcgatatcg ttggcctggt
    14881 ttaaggaacg cgcttcagcc agcaattgct gctcgcgctt aagacgacgc ttctgattga
    14941 agaactccac gctcttactg aagaagattg cccaggtgac tacggaggcc aaaataagcc
    15001 caatcatcac gcacttaacg acaatatcgg cgtgctgata cataccccag acggagaggt
    15061 ccgtctgcat taaattatta cccactgtgt atctccagga cgcaagtcac aaaatctgtg
    15121 cataataata tcaaaacgac gtcgaattga tagtcgttct cattactatt tgcatactgc
    15181 cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag cacgccattt
    15241 gcgaaaattt tctgctttat gccaattctt caggatgcgc ccgcgaatat tcatgctagt
    15301 ttagacatcc agacgtataa aaacaggaat cccgacatgg cggacaaaaa gcttgatact
    15361 caactggtga atgcaggacg cagcaaaaaa tacacgctcg gtgcggtaaa tagcgtgatt
    15421 cagcgcgcgt cttcgctggt ctttgacagt gtggaagcca aaaaacacgc gacacgtaat
    15481 cgcgccaatg gcgagttgtt ctatggacgg cgtggaacgt taacccattt ctccttacaa
    15541 caagcgatgt gtgaactgga aggtggcgca ggctgtgtgc tatttccctg cggggcggca
    15601 gcggttgcta attccattct tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac
    15661 accgcctatg aaccgagtca ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg
    15721 acatcgtggt ttgatccgct gattggtgcc gatatcgtta agcatctgca gccaaacact
    15781 aaaatcgtgt ttctggaatc gccaggttcc atcaccatgg aagtccacga tgttccggcg
    15841 attgttgccg ccgtacgcag tgtggtcccg gatgccatca ttatgatcga caacacctgg
    15901 gcagccggtg tgctgtttaa ggcgctggat tttggcatcg atgtttctat tcaggccgcc
    15961 accaaatatc tggttgggca ttcagatgcg atgattggca ctgcggtgtg caatgcccgt
    16021 tgctgggagc agctgcgaga gaatgcctat ctgatgggcc agatggtcga tgccgatacc
    16081 gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgcca acatcatgaa
    16141 agcagtctga aagtggctga atggctggca gaacatcccc aagtagcgcg agttaaccac
    16201 cctgctctgc ctggcagtaa aggccacgaa ttctggaaac gagactttac aggcagcagc
    16261 gggctatttt cctttgtgct taagaaaaaa ctcaatgatg aagagctggc gaactatctg
    16321 gataacttca gtttattcag catggcctac tcgtggggcg ggtatgaatc gttgatcctg
    16381 gcaaatcaac cagaacatat cgccgccatt cgcccacaag gcaagatcga ttttagcggg
    16441 accttgattc gcctgcatat tggtctggaa gatgtcgacg atctgattgc cgatctggac
    16501 gccggttttg cgcgaattgt ataacattgc cacttttgga caattttgca gacattttat
    16561 tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt cccggacgat tcaggagtac
    16621 aataggcaga taaaggctta aacgctgttc cacaggaaag tccatggctg ttattcaaga
    16681 tatcatcgct gcgctctggc aacacgactt tgccgcgctg gcggaccctc atattgttag
    16741 cgttgtttac tttgttatgt ttgccacgct gtttttagaa aacggcttgc tgcccgcctc
    16801 atttttgcca ggcgacagct tgttgatact ggcaggcgca ttgattgccc agggggttat
    16861 ggattttctg cctacgattg cgattctgac cgccgcagca agtctgggct gctggctaag
    16921 ttatattcag gggcgctggt tagggaatac caaaacggtg aaaggctggc tggcacagct
    16981 tcctgctaaa tatcaccagc gcgccacctg catgtttgac cgccacggtc tgctggcgct
    17041 gctggctgga cgttttcttg catttgtccg tacgctgctg ccaaccatgg cgggaatttc
    17101 cggtctgcca aaccgccgct tccagttttt caactggtta agcggattgc tgtgggtcag
    17161 cgtggtaacc agtttcggct atgccttaag tatgattccg ttcgttaaac gacatgaaga
    17221 tcaggtaatg acgttcctga tgatcctgcc aattgccttg ttaaccgctg gcttgttggg
    17281 cacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc
    17341 ggttgctgta ccgggaacgt atttaattcc cctgcatcgc ccgcattctt gccgcatctt
    17401 cccccggcgt cacaccgaag taacgtttaa actcacggct aaattgcgat gcgctttcat
    17461 agccgacgcg catcgccgcc gcgctggcct tcatgccgtc gtggatgatc atcatccgcg
    17521 ccttatgcag acggtaattc ttcaaatact gcaacggcga ggtgctggtg acagacttaa
    17581 aattatggtg gaacgccgat acgctcatgt tggcttctgc cgccagttgc tcgacgctca
    17641 ggttttcggt gtatttattc tcaatccgtt tcagcacgcg actaatcagg ctgaagtgag
    17701 tctggcggct gaccagtgcc agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca
    17761 gaatttcgcg gataatctgt ttgccgagaa tgcgcgcatc cagcggtcgc tccatcacat
    17821 ccagtaaccg ctctgccgcg cataaaatct cttctgataa cgtggcggag ttaataccgc
    17881 tggctgccat tgacggctga aaatgttcat cttcgccaat gtccatcaac agttcctgta
    17941 actgcaaaat atcgacattg agacgcaacc ctgccagcgg cacctctgac gtggcatagg
    18001 tttcgcactc aaacggcaac ggcaccgtca gcaggagata ttcattagcg tcataacgaa
    18061 acacgcgttc attgatataa ccgattttat gtccggaaaa gagaattatg atgccaggct
    18121 cgtacatcac cggtgtgcgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca
    18181 acagctctga caaactattt tctttatttt tcagtttatt aactttatcc gccagcaagc
    18241 ggcaaatctc ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt
    18301 gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa taggcaagac attggcagaa
    18361 atgagcattg agaggtacgg cgctggcgac cacaatgaaa aacatcaggc agaccgatct
    18421 ctgccctcat attgacccag caaagggagc aagtaatgaa caactttaat ctgcacaccc
    18481 caacccgcat tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg
    18541 atgctcgcgt attgattacc tacggcggcg gcagcgtgaa aaaaaccggc gttctcgatc
    18601 aagttctgga tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gaaccaaacc
    18661 cggcttatga aacgctgatg aacgccgtga aactggttcg cgaacaaaaa gtgacttttc
    18721 tgctggcggt tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta
    18781 actatccgga aaatatcgat ccgtggcaca ttctgcaaac gggcggcaaa gagattaaaa
    18841 gcgccatccc gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgcag
    18901 gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc gttccattct gcccatgttc
    18961 agcccgtatt tgccgtgctc gatccggttt atacctacac cctgccgtcg cgtcaggtgg
    19021 ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg
    19081 atgccaaaat tcaggaccgt ttcgcagaag gcattttgct gacgctgatc gaagatggtc
    19141 cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga
    19201 ctcaggcgct gaacggtttg atcggcgctg gcgtaccgca ggactgggca acgcatatgc
    19261 tgggccacga actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc
    19321 tgcctgcact gtggaatgaa aaacgcgata ccaagcgcgc taagctgctg caatatgctg
    19381 aacgcgtctg gaacatcact gaaggttcag acgatgagcg tattgacgcc gcgattgccg
    19441 caacccgcaa tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg
    19501 acggcagctc catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg
    19561 gcgaaaatca tgacattacg ctggatgtca gccgccgtat atacgaagcc gcccgctaag
    19621 ctttttacgc ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc
    19681 tcaccggagc ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt
    19741 tattaagcta caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag
    19801 taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga
    19861 taccgccgca gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt
    19921 caacagagaa gaactgttca tcaccactaa gctgtggaac gacgaccaca agcgcccccg
    19981 cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc tctatttaat
    20041 gcactggccc gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt
    20101 gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca
    20161 acgcctgatt gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcacccgct
    20221 gatgcaacaa cgccagctac atgcctggaa cgcgacacac aaaatccaga ccgaatcctg
    20281 gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc
    20341 agataaatac ggcaaaaccc ctgcgcagat tgttatccgc tggcatctgg atagcggcct
    20401 ggtggtgatc ccaaaatcgg tcacaccttc acgcattgtc gaaaactttg atgtctggga
    20461 tttccgtctc gacaaagacg aacttggcga aattgcaaaa ctcgatcagg gcaagcgtct
    20521 cggtcccgat cctgaccagt tcggcggcta acgtgcaaat tatcccggtg gcggtaatgt
    20581 tccgctaccg gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgctactct
    20641 tccttgttca ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa
    20701 gaacaaccca tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa
    20761 tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca cgcacagccc ccctcacaga
    20821 ccgtagaaca aacagtccgg catatttatc agaactataa atcagatgcc actgcccctt
    20881 attttggtga aaccggagag cgggcgataa catctgcgcg tattcaacag gcgcttaccc
    20941 tgaacgacaa tcttacgctg ccgggcaata ttggctggct ggattatgat ccggtttgtg
    21001 attgtcagga ttttggcgat ctggtgttag aaagcgtagc gataacccaa actgacgccg
    21061 atcatgccga tgccattgtg cgctttcgta tctttcaaga cgataaagaa aagacctcgc
    21121 agacactgaa aatggtggcg gaaaatggtc gttgggtcat tgacgatatt gtcagcaatc
    21181 atggcagcgt cttacaagcg gttaatagcg agaatgaaaa aacgctggcc gctatagctt
    21241 cgttgcaaaa agaacagccg gaagcctttg ttgccgaact ctttgaacat attgctgatt
    21301 atagctggcc gtggacgtgg gtggtttccg actcttaccg ccaggcggtc aatgccttct
    21361 ataaaaccac cttcaagacg gccaacaatc ccgatgaaga tatgccaata gaacggcaat
    21421 ttatttacga caatccgatc tgttttggcg aagagtcgtt attttcccgc gttgatgaaa
    21481 ttcgagtcct ggggaaaacc accgattccg cccgcattca tgttcgcttt acgctgacca
    21541 atggcaacaa cgaagagcaa gaactgattt tacagcggcg cgaaggtaag tgggaaatcg
    21601 ctgattttat ccgcccgaac agcggcagcc tacttaagca gattgaggca aaaaccgccg
    21661 ccagattaaa acaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga
    21721 acactatatg aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag
    21781 tggctgcgtc aatgagcaaa aggtcaatca gctggcgagc aatgtgcaaa cattaaatgc
    21841 caaaatcgcc cggcttgagc aggatatgaa agcattacgc ccacaaatct atgctgccaa
    21901 atccgaagct aacagagcca atacgcgtct tgatgcacag gactatttcg actgcctgcg
    21961 ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg cagcattctg ttaccgggga
    22021 tattttttaa cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tctgcaggcg
    22081 agattgcgta gacgacgctt tttttgccgt agcaggcgtc tgacgctggg tcgccatcgg
    22141 cgtatgttta gtcaacgccg gacgggtatt gcggttctga cggcgagctt cacgcatctc
    22201 ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc
    22261 catcgcttcc agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa
    22321 cgctttatgc aggcgacgct gcttgtcgcc cttcggtacg aagacgtctt cactcttata
    22381 accaatcttc gccagcgggt ttttgccggt gtaatacatg gtggtcgagt tagccagcgg
    22441 cgacggatag aagttctgca cctggtcgag gcggaagcga tgctttttca gccatagcgc
    22501 cagattcacc atatcttcat cacgcgtacc cgggtgcgcg gagatgaaat acgggatcag
    22561 atactgttct ttacctgcct gtttcgagta agtatcgaac agctctttaa agcggtcata
    22621 gctgcccatg cccggcttca tcatcttcga taacggccct tcttcggtat gttccggggc
    22681 aatcttcaga taaccgccaa cgtgatgggt cgccagctcg ttgatatagc gcggatcttc
    22741 tacggcgatg tcataacgca caccagaggc aatcaggatc tttttaatgc ctttcagatc
    22801 gcgcgcacgg cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatat gcggacaaat
    22861 atccggataa acgcacgaca aacgacggca ggtttgctca gcgcgtggcg atttgcaacg
    22921 caacatatac atgttggcag taggcccacc gagatcggaa atcacgcccg taaaacctgg
    22981 aacagtgtcg cggatcgctt cgatctcatt aataatcgaa tcttcggaac ggctctgaat
    23041 aatgcgccct tcgtgctcgg taatagaaca gaaagagcag ccgccaaagc agccacgcat
    23101 aatgttgacc gagaaacgaa tcatttcgta agccggaatt cgggcattac cgtaagccgg
    23161 atgcggcacg cgcttgtacg gcagcgcaaa gacgctatcc atctcttcgg tagaaagcgg
    23221 aatagcaggc gggttgatcc atacatagcg gtcgccatgt ttttgcatca atgcgcgggc
    23281 gcagcctggg ttagtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc
    23341 gcccttcact ttctcgaaag aaggcagcaa cacgtaggtt ttttcccacg gtttcgggcg
    23401 tggtggctgc acggttacag ctttggcttc ctgctttttc ggagctaccg gtttgttatc
    23461 cgcgcacggc aaatcttcgc catacggatg cgggattggg tcgattttcc ctggggtatc
    23521 aagacgggtg gaatccacac cgctccagcc tggcaacgcc tcttttacga taatcgcggt
    23581 attacgcaca tcgcggattt cactaatcgg ctcgcccatc gccagacggt gcgccacttc
    23641 caccagcgga cgctcaccgt taccaaacat cagcatgtcg gctttcgaat ccaccagcac
    23701 ggaacggcgc acggtatccg accagtaatc atagtgcgcg gtacggcgca ggctggcctc
    23761 aataccgccg aggatcaccg gcacatcttt ccacgcctct ttacaacgtt gggtataaac
    23821 cagtgtggcg cgatccggac gcttacccgc gacgttatcc ggtgtgtagg catcgtcatg
    23881 acgtaaacgg cgatcggcgg tatagcggtt gatcatcgag tccatgttgc cagcagtaac
    23941 accgaaaaac agattcggtt tacccagacg cataaagtcg tctttactgc tccagtctgg
    24001 ctgggcgatg atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat
    24061 cccgaagctt gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc
    24121 ccagccaagt tgatccatct cttcacgtga catcggcaaa aacggtgccg gtccaaaaca
    24181 ggcggcccag tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga
    24241 gctcattttg cttccagaaa tggtaaaaaa ataatcaaag gccggggatt atacgctgta
    24301 tcacgcgcgg aaatgaagtg atgttgatga acggtggcga aataccctgg tgaggttttt
    24361 cactgcccga cttgggcaag cacatttaag gctggcggta tcgtggcaac tgccgctact
    24421 atttatgccg atcagcaacg ttcaaatcat attgcacgac gatcggctat ccgaggacac
    24481 ccacagtaag gcaaggtatg gaacaggtta ttacaaagcg ccgctattac gatattggtc
    24541 tacagattga agagctgctg tattcaggcg tatttaaggc cggagaacgc ttaccttcag
    24601 aacgtgaact gagcgagcgg tttaatacca gccgaactac cattcgtgaa gccatcatta
    24661 tgctggaact gaaaggcgta ttgaatgtga agcaagggtc agggattttc tttgtcgact
    24721 ccaccgataa gctcaaccag aaatcattaa tgccctattc agaaattggc ccgtttgaac
    24781 tgttacaggc acgtcaggta attgagagca atattaccgg ttttgctgcc agccagatca
    24841 gctttaacga gttacaagag ttgaagaaaa tcattggatt acaggaaaaa gccatcgctg
    24901 cggaaagtga taaatttgaa gacctggatc accgatttca tagcattatt gccgaagcga
    24961 cgcaaaatcg ggtactcatc aaacaagctg ctgaattatg gcgggcagtt cgtacagaaa
    25021 acccccgctg gaagaaactt aactacaagt acctgcatga aaagcatttg cgtctgcaat
    25081 ggctggaaga tcaccgggct attttcctgg ctctgcaaca gaaagattca gagctcgcca
    25141 gggaagcgtc ctggagacat ctggaaaaca gcaaaaatga actgatcaaa atcttcaaac
    25201 aagacgcttc catcagcgat tttgatgact ttttcttcgc tcgttaacct tcattagtcc
    25261 agagctggcg gaaaaaataa cgccggctct gccttccgcc ctccactggt acacgctctc
    25321 tttttaattg gt
//
LOCUS       DAZFRG010000046        25207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFRG010000046 DAZFRG010000000
VERSION     DAZFRG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.046"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..526
                     /locus_tag="ACHKUC_001481"
     CDS             <1..526
                     /locus_tag="ACHKUC_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFO6617763.1"
                     /translation="SGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEWEKLDAADAI
                     IFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDKQVTLIYLQT
                     LASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQIPTGDLDTAV
                     KYGERVATITARLK"
     gene            complement(704..1696)
                     /gene="rpoS"
                     /locus_tag="ACHKUC_001482"
     CDS             complement(704..1696)
                     /gene="rpoS"
                     /locus_tag="ACHKUC_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO6617764.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1759..2853)
                     /gene="nlpD"
                     /locus_tag="ACHKUC_001483"
     CDS             complement(1759..2853)
                     /gene="nlpD"
                     /locus_tag="ACHKUC_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO6617765.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3038..3664)
                     /gene="pcm"
                     /locus_tag="ACHKUC_001484"
     CDS             complement(3038..3664)
                     /gene="pcm"
                     /locus_tag="ACHKUC_001484"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO6617766.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3658..4419)
                     /gene="surE"
                     /locus_tag="ACHKUC_001485"
     CDS             complement(3658..4419)
                     /gene="surE"
                     /locus_tag="ACHKUC_001485"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO6617767.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4400..5449)
                     /gene="truD"
                     /locus_tag="ACHKUC_001486"
     CDS             complement(4400..5449)
                     /gene="truD"
                     /locus_tag="ACHKUC_001486"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO6617768.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKVLMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5446..5925)
                     /gene="ispF"
                     /locus_tag="ACHKUC_001487"
     CDS             complement(5446..5925)
                     /gene="ispF"
                     /locus_tag="ACHKUC_001487"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO6617769.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5925..6635)
                     /gene="ispD"
                     /locus_tag="ACHKUC_001488"
     CDS             complement(5925..6635)
                     /gene="ispD"
                     /locus_tag="ACHKUC_001488"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO6617770.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLVNHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6654..6965)
                     /gene="ftsB"
                     /locus_tag="ACHKUC_001489"
     CDS             complement(6654..6965)
                     /gene="ftsB"
                     /locus_tag="ACHKUC_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO6617771.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7158..7481)
                     /gene="ygbE"
                     /locus_tag="ACHKUC_001490"
     CDS             complement(7158..7481)
                     /gene="ygbE"
                     /locus_tag="ACHKUC_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO6617772.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7531..8136)
                     /gene="cysC"
                     /locus_tag="ACHKUC_001491"
     CDS             complement(7531..8136)
                     /gene="cysC"
                     /locus_tag="ACHKUC_001491"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO6617773.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8136..9563)
                     /gene="cysN"
                     /locus_tag="ACHKUC_001492"
     CDS             complement(8136..9563)
                     /gene="cysN"
                     /locus_tag="ACHKUC_001492"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO6617774.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9565..10473)
                     /gene="cysD"
                     /locus_tag="ACHKUC_001493"
     CDS             complement(9565..10473)
                     /gene="cysD"
                     /locus_tag="ACHKUC_001493"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO6617775.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10725..11762
                     /gene="iap"
                     /locus_tag="ACHKUC_001494"
     CDS             10725..11762
                     /gene="iap"
                     /locus_tag="ACHKUC_001494"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO6617776.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11887..12039)
                     /locus_tag="ACHKUC_001495"
     CDS             complement(11887..12039)
                     /locus_tag="ACHKUC_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6617777.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12304..13038)
                     /gene="cysH"
                     /locus_tag="ACHKUC_001496"
     CDS             complement(12304..13038)
                     /gene="cysH"
                     /locus_tag="ACHKUC_001496"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6617778.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNVQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13112..14824)
                     /gene="cysI"
                     /locus_tag="ACHKUC_001497"
     CDS             complement(13112..14824)
                     /gene="cysI"
                     /locus_tag="ACHKUC_001497"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO6617779.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14824..16623)
                     /gene="cysJ"
                     /locus_tag="ACHKUC_001498"
     CDS             complement(14824..16623)
                     /gene="cysJ"
                     /locus_tag="ACHKUC_001498"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO6617780.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            16942..17304
                     /gene="queD"
                     /locus_tag="ACHKUC_001499"
     CDS             16942..17304
                     /gene="queD"
                     /locus_tag="ACHKUC_001499"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO6617781.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            17382..18653
                     /gene="ygcN"
                     /locus_tag="ACHKUC_001500"
     CDS             17382..18653
                     /gene="ygcN"
                     /locus_tag="ACHKUC_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6617782.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCTRAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLLPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18644..18904
                     /gene="ygcO"
                     /locus_tag="ACHKUC_001501"
     CDS             18644..18904
                     /gene="ygcO"
                     /locus_tag="ACHKUC_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6617783.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18921..19496
                     /gene="ygcP"
                     /locus_tag="ACHKUC_001502"
     CDS             18921..19496
                     /gene="ygcP"
                     /locus_tag="ACHKUC_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO6617784.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19644..20504)
                     /gene="ygcQ"
                     /locus_tag="ACHKUC_001503"
     CDS             complement(19644..20504)
                     /gene="ygcQ"
                     /locus_tag="ACHKUC_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6617785.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLTEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20501..21280)
                     /gene="ygcR"
                     /locus_tag="ACHKUC_001504"
     CDS             complement(20501..21280)
                     /gene="ygcR"
                     /locus_tag="ACHKUC_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO6617786.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVVLETAADLRFASE
                     FVARHIAEWQRQNPLDLIIIGCQSSEGQNEQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQNYLRQRMQP"
     gene            complement(21258..22667)
                     /gene="ygcS"
                     /locus_tag="ACHKUC_001505"
     CDS             complement(21258..22667)
                     /gene="ygcS"
                     /locus_tag="ACHKUC_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6617787.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22689..24143)
                     /gene="ygcU"
                     /locus_tag="ACHKUC_001506"
     CDS             complement(22689..24143)
                     /gene="ygcU"
                     /locus_tag="ACHKUC_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO6617788.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(24213..24998)
                     /gene="ygcW"
                     /locus_tag="ACHKUC_001507"
     CDS             complement(24213..24998)
                     /gene="ygcW"
                     /locus_tag="ACHKUC_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6617789.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
BASE COUNT         5960 a         6873 c         6291 g         6083 t
ORIGIN      
        1 ttcaggatat ggtcacacta aactggttgc tgaaaaagtg gcacaaggcg cccatgcaga
       61 tttaatttcg attgataacg aaggaaatat cactgagcag gagtgggaaa aactggacgc
      121 agccgatgcc attatcttcg gtgctcccac ctatatgggc ggtgcgccat ggcaattcaa
      181 gaaatttgct gatgccagtt ccaaagcgtg gtttacacgt aaatggcagg ataaagtctt
      241 cgggggtttc accaacagcg ccagcctgaa tggcgataaa caagttacgc tgatctatct
      301 gcaaacgctg gcttcacagc atggcggtct gtgggtgagt ttaggtcagg ctcctgccaa
      361 tgtgcttgcg tcaaccaggg aagatgtaaa caatctgggc ggttcagtcg gtttgctggt
      421 gcaatctcca gccgatgcgg gtgcagacca aatccctacg ggcgatctgg atacagccgt
      481 gaaatatggt gaacgcgtgg cgacaattac tgcgcgctta aaatgatgat tgccgaatgt
      541 gacgctgacg cgtctatccg gtcttctaca tctaaaaagt gccgtcccct ggtcagtcag
      601 ataaagtttt aacgctgtat ccgataacag ttcaacatgt accatgtacc aaaagaaaaa
      661 aagccagcct cgcttgagac tggcctttct gacagatgct tacttattcg cggaacagcg
      721 cttcgatatt cagcccctgc gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct
      781 gaatctggcg aacacgttca cgggtgaggc caatttcacg acctacatct tccagtgttg
      841 ccgcttcgta ccccagcaaa ccgaatcgac gtgccagcac ttcacgctgt ttggcgttca
      901 gctcgaacag ccatttgacg atgctctgct tcatatcgtc atcttgcgtg gtatcttccg
      961 gaccattctc tttttcatcg gccaggatgt ccagcaacgc tttttcggaa tcaccaccca
     1021 gcggggtgtc taccgaggta atgcgctcgt taagacgaag catacggctg acgtcatcaa
     1081 ctggcttatc cagttgctct gcgatctctt ccgcacttgg ttcgtggtcc agcttatggg
     1141 acaactcacg tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga atcggcaaac
     1201 gaatagtacg ggtttggttc ataatcgccc gttcgatcgt ctggcgaatc caccaggttg
     1261 cgtatgttga gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc
     1321 ccaggttgcc ctcttcgatc aggtccagca acgccagacc acgattgcca taacggcggg
     1381 caatttttac caccagacgc aagttactct cgatcatccg gcggcgagag gcgacatctc
     1441 cacgcagtgc gcgacgcgca aaataaactt cttcttcggc cgttaacagt ggtgaataac
     1501 caatctcacc aaggtaaagc tgagtcgcgt ccagcacacg ctgtgtggct ccctgcgata
     1561 acagttcctc ttcggccaaa tcgttatcac tgggttcctc ttctactaag gccttttcgt
     1621 caaaaacctc aactccgttc tcatcaaatt ccgcatcttc atttaaatca tgaactttca
     1681 gcgtattctg actcataagg tggctcctac ccgtgatccc ttgacggaac attcaagcaa
     1741 aagcctggtt ccgccgattt atcgctgcgg caaataacgc agcgggttta cggatttccc
     1801 cttgtaacga atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc
     1861 tattttttgc cccgccttca cttcttgttg ttcccggacc agcattgtgt cgttatgggc
     1921 gtaggcactc aggtaatcat cattatgttt gatgataatc agattaccgt agccgcgcag
     1981 cgcgttaccg gcataaacaa cgcggccatc tgcggtcgcg ataattgcct gtcctttgct
     2041 gcctgcgata tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt
     2101 gccctcagtc ggccagcgcc aggtggagat aggcgtactg gttgatgtac tgctgacagt
     2161 cggctcggtt gtgcttgctg ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg
     2221 cttgttgttc ggcaacattt tgttagcact ctgttcaccc gaagactcag aatacgtaat
     2281 tgtcggttgc gacgcaacag caacggtgga attttgtgca ggcttgatca caactccttg
     2341 ctctgctgcg tcggcctggg taatggcatt tccgcctgtg attggcgtac cggaagcatt
     2401 acccacctgt aaggtctgac cgacgttcag cgcgtatggt gcctgaatat tgttgcgctg
     2461 agcaaggtca cggaaatcgt tgccagtaat ccaggcgata taaaaaagcg tgtcgccttt
     2521 tttcacggtg tatgtactgc cgctataact gcctttcgga atgttcccat actgacggtt
     2581 gtagacgatt cgtccgtttt ccatctgtac cggctgctga gctactggct gcacaggctg
     2641 gatttgcggt tgttgagtag cctgaatttg tggctgctgt accggctgaa tttgcggttg
     2701 ctgcgctgta gacgtcgtcc ccattttcgg cggcggcgta atcaacatac cagaattggt
     2761 atttgcgggc gcattgccat taacggagct gaccggtgcc ggtggatttg aagtgtcaga
     2821 acagcctgcc agccatagcg aaaccagtga caatgccgca atgcggcgaa cggtgaattt
     2881 tgggcttccc gcgctcattt atcccccagg aaaaattggt taataaccag tgacataatt
     2941 accgtgcaag gcaccatact gaacactgga aaagatgttc acgatacgct gacttgcggc
     3001 aaaataacca ggaaaaatcc aggtatttcc tcacgtttta agccagctca cccttcacta
     3061 aagggacaaa gcgcacggcc tccacggtat cgataataaa ttcgcctccc cgacgacgca
     3121 cccgttttaa atactggtgc tcctccccta cgggtaagac gagaatcccg ccttcgtcca
     3181 gctgcgtcat tagcgcagtt ggaatttcag gcggtgccgc cgtaacaatg atagcgtcaa
     3241 acggcgcgcg tgcctgccaa ccttgccatc catcgccatg acgggttgaa acattatgta
     3301 aatcaagatt tttcaggcgg cgacgcgcct gccactgcaa gcctttaatc cgttcaaccg
     3361 agcaaacatg ttggacaaga tgcgccagga ttgcagtttg atatcccgaa ccagtgccaa
     3421 tttccagcac ccgcgactgc ggcgtcagct cgagtaattc ggtcattcgc gccaccatat
     3481 acggctgcga aattgtctgc ccctgaccta tcggcaaggc gatattgtcc caggcttttt
     3541 gttcaaacgc ttcatcaacg aatttttcac gcggcacggc ggcaagtgca ttcagcacca
     3601 gctcatcctg aataccttgc gcacgtaatt gatccagaag tgcttgtacg cgtctgctta
     3661 ccattgcgtg ccaactccca cgctgtttaa ccagtctgaa accacatctt gcgcgctatg
     3721 cgcagttaaa tccacatgca gcggcgtgat ggaaacatag ccctcatcta ccgcagcaaa
     3781 atcggtcccc ggaccggcat cacatttacc gcccggcggg ccaatccagt acagcgtatt
     3841 gccgcgcgga tcttgctgcg ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt
     3901 cacgcgaata cctttgattt gatccaaggg taaatccgga acgttaatat taagaatacg
     3961 cccggtgcgc agaggctctt tacacagtgc gcgcaaaatt gagcaggtta ccgccgcggc
     4021 agtgtcgtaa tgtttatgcc cgtcaagcga gacggcaagc gccggaaaac ctaaatgacg
     4081 gccttccatc gcggccgcta ccgtaccgga ataaataaca tcatccccca gattcggccc
     4141 ggcgttaatt ccggacacaa caatgtccgg gcgcgggcgc atcagagcat ttacgccaag
     4201 atagacgcaa tcggtcgggg ttcccatttg cacagcaata tcaccatttt caaaggtaaa
     4261 cgtgcgcagg gaggattcca gtgtcagaga attagaagcg ccgctgcggt tacgatcggg
     4321 ggcgaccacc tgaacgtcag caaactcacg caaggctttc gccagcgttt gtataccggg
     4381 tgcatgtacc ccgtcatcat tactcagcaa tatgcgcata atcacctgtt gtgttgataa
     4441 gttccctgac aacgctggtt gcaaaacttc ccgccggaag ccagaaacgg atttctacgg
     4501 tgacatcatc ccaccagttc cagcttaatt gttgcggata cagcagcatc gctctgcgcg
     4561 cggcttcaac tttttcgcgc accagtaagg tttgtaattc agtttcttcg gcgacagctg
     4621 cttgttcgaa tgccagcgct tcacgctgag ttccccactc gccactgccc ggcaatgcgg
     4681 cggttatcat cagcacttta tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg
     4741 ttgcgacaaa ccagctacca cgtccggcta attgtagcgc atcgccgtca acaacttgat
     4801 taacgtctgc ttttttgagg cgttcagcaa caatctgatt aaacaacgca ctgcgggctg
     4861 ccgacaacca aaaactccgt ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc
     4921 gcagcgcacc ctgcaagttg ctaccgccaa tcccaaaacg ttgggcacca aagtagttcg
     4981 gtacaccttt tacgcaaata tcgatcagac gttgttcaac gtcatcgcga ttgctcactt
     5041 cgcgtagtac cagggtaaag gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt
     5101 gccgcgcata ctccagcacc tggcagcctt ccagttgaaa ggcgctcaga tcgggcatct
     5161 ctttgcccgg cacgcgagcg cataaccact gttccgtaac agcatgttta tctttttgcc
     5221 cagcgaagct gacttcacgg gcatgaattt tcaggaattt cgccagtgca tccgccacaa
     5281 aacgggtatt gcagccgttt ttgaggattc taaccagaat atgctcacct tcaccatcag
     5341 gctcaaagcc caaatcttcc accaccacaa agtcttccgg attggctttc agcagcccgg
     5401 tgccttgcgg tttaccgtgg aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt
     5461 aatgagtagc gccaccgctt cacaggcaat cccttcccca cgtccggtaa atcccagttt
     5521 ttccgtagta gtggctttca cgttaacatc atccatatgg cagccgagat cttcggcaat
     5581 aaacacgcgc atttgtggaa tgtgcggcaa catcttcggt gcctgagcga tgatagtgac
     5641 atcgacgttg ccgagggcat aacctttcgc ctgaatacga cgccaggctt cgcgcaacag
     5701 ctcgcggctg tcggcacctt taaatgccgg atcggtatcc gggaacagct tgcctatatc
     5761 tcccagcgcc gccgcgccaa gcaatgcatc ggtcaacgca tggagcgcca cgtcgccatc
     5821 agaatgcgcc agcagccctt tttcgtaagg aatgcgtacg ccaccaatga taattgggcc
     5881 ttcaccgcca aaggcgtgta cgtcaaaacc gtgtccaatt cgcattatgt attctcctga
     5941 tggatggttc gggtgaggta aaattcggcc agcgccaaat cctccgggcg cgttaccttg
     6001 atgttatccg cgcggccttc aacaagttgc ggatgaaaac cgcaatattc cagcgccgag
     6061 gcctcatcgg taatggtagc gccttcattt agagcgcgcg tcaggcagtc atgtaacagc
     6121 tcacgaggga aaaattgcgg cgtcagcgcg tgccataagc cgttgcgatc aacggtatga
     6181 gcaatggcat ttttgcccgg ttcggcacgt ttcatcgtat cgcgcaccgg cgcagcgagg
     6241 atccctcccg tgcggctggt ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc
     6301 agacaaggac gagcggcgtc atgcaccaat acccactgcg cgtcgccagc ggctttcaga
     6361 cctgccagca cggaatcggc acgctcttca ccaccatcaa caaccgtgat tcgtggatga
     6421 ttcaccagag gaagttgtgc aaagcggcta tcgccaggac ttatggcaat gacgacacgt
     6481 ttcaccctgg gatgcgccag cagcgcatgc accgagtgtt caagaatggt ttgattaccg
     6541 attgagagat attgcttagg acattccgtt tgcattcggc ggccaaatcc ggccgccgga
     6601 accacggcgc aaacatccaa atgagtggtt gccatgttaa ttcccgggct gatttatcga
     6661 ttgttttgcc ccgcagactg tgcgcgcttt gacgcgtcag gcaccagacg ataaaacgtt
     6721 tcgcccggcc tggtcatgct gagttcatta cgcgcacgct cttcgagcgc ctcctggccg
     6781 ccattgagat cgtcaatttc ggcaaaaagc tgatcgtttc gcgctttaag tttcgcgttt
     6841 gtagcttgct gtgccgccac atcattattg acgcgagtat agtcatgtat accgttctta
     6901 ccgaaccaca gcgaatactg tagccagacc agaatagcca gcaacagcag cgttagttta
     6961 cccatcctgc cccctgaaaa acggcatctt catcccatgc atccgaagac gactctacat
     7021 cctctgttgg ggataccgcg acaacgcggg caaatgtacc acatttgtcc attgttacgt
     7081 atacccaggg cgtgcagaac ataatctcat tattagttac ggattgaatt atgaacagaa
     7141 tagagaggaa agaacaatta acccagtagc cacataaaca gcgcaccaaa cataatgcca
     7201 accgtcgcca gagtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagt
     7261 gcaatcccca ccaccactgc gacgggcatc agcgccagaa agaaaggcca ggtgtagata
     7321 aaaaagagca gcgtgttaga gccataaatc aacatcggaa ttgccaacgc gcacagccac
     7381 gataaaaaac caaccacagc gcctggcagc gaccatgtgg tgtcttcaac ctcagtaagg
     7441 ctgtcgttat ttgttaatgt aatgttgtgg ctgttacgca tatctgatcc tgttactttg
     7501 acgaaccggg catggaaacc cggcggtgtc tcaggatctg ataatatcgt tctgtctcaa
     7561 cagatctaat aattgctgta ccaaatttgt tactaattgt tcaccattga gatgaatttc
     7621 tgccgattca ggcgcttcgt aaacggaatc tattcccgta aagttgcgca gttcaccggc
     7681 acgcgctttc ttatataaac cttttggatc gcgggcttcg caaatcgcca gcggcgtatc
     7741 gacaaacact tcgataaagc gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc
     7801 gcggtgtggc gagataaatg cggtcagcac caccagtccg gcttcaacca tcaaattcgc
     7861 cacttcaccg acgcgacgga tattctcttt acggtcggca tcgctaaaac cgagatcgct
     7921 gcataatccg tggcgaacgt tgtcgccatc cagcagatac gtactgacgc caagtttatg
     7981 taacgcctcc tccagcgccc ccgcgacggt tgatttaccg gaaccagaga ggccggtaaa
     8041 ccacagcaca acaccacgat gaccgtggtg ctgttcgcgt tgttgcggag ttactgggtg
     8101 gctatgccag acgacgtttt cgtcatgcag cgccattatt tatctcccag taaatcgcgc
     8161 gcaccccaat gcgggaaatg gcggcgaacc agggcattca attccagttc gaatgcactg
     8221 aattcagatg gcgcagcagt tgcctggcta actggttcgt gcaccatacc ggcacctacg
     8281 gtcacattac tcaggcgatc gataaaaatc agcccaccgg taaccgggtt ttgctgataa
     8341 cgatctaaca ccagtggctc gtcaaaggtg agatctacca ggccgatgcc gttcagcggc
     8401 aggttttcaa cttcgcgttg ggtaaggtta ttgatatcaa cctgataacg aatgccatca
     8461 acacgagcac gcgtcttctt accggcaatt ttgatgtcgt aactctggcc cggggaaagc
     8521 ggctgttccg ccatccatac cacatccacc gacgcgctct gcacagccgg taacgcttcg
     8581 tctgccgcca gcagcagatc gccacggctg atgtcgatct catccgtcag caccagggtg
     8641 atcgcttctc cggcaaaggc ttcttcgcga tcgccatcaa aggtcacgat ccgcgcgacg
     8701 tttgattcca caccagaggg cagcactttg acacgttgcc cgacttccac gcgaccggat
     8761 gccagcgttc cggcgtagcc acgaaaatcg agattcggac ggttaacgta ctgcaccggg
     8821 aagcgcattg gctgagcatc caccactcgc tggatctcca cggtttccag cacttctagc
     8881 agtgtcggac cgctgtacca cggcatactt tcactttgcg aagccacgtt gtcgccttcc
     8941 agtgcggaga gcggcacaaa gcggatatcc agattacccg gcagctgccc ggcaaaagtc
     9001 agataatctt cacgaatacg ggtgaacgtc tcttcactgt aatccaccag atccattttg
     9061 ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg agataaaact gtgacgacgg
     9121 gtttgatcga gcacgccttt acgggcatcg atcagtaaga tcgccagttc acatgtcgat
     9181 gctccagtcg ccatattgcg ggtgtactgc tcgtgccctg gggtgtcggc gataataaat
     9241 ttacgcttct cggtagagaa atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc
     9301 tcagcttgca ggccgtccac cagcagtgcc agatccagct tttcgccctg ggtgccgtga
     9361 cgcttactgt cgttatgcag cgatgagagc tgatcttcgt agatttggcg ggtatcgtgt
     9421 agcaggcgac caatcagggt acttttgccg tcatcgacac taccacaggt cagaaaacgc
     9481 agcaggcttt tatgttgttg cgcaatcatc caggcttcga cgccgccttc attggcgatt
     9541 tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga cgttttttca gctccataga
     9601 ccccgcctgg tcgcggtcaa ttacgcggcc ctgacgttca ctggtggtgg aaaccagcat
     9661 ctcttcgatg atctccggca gtgtttgcgc atttgactcc accgcgccgg tcagcggcca
     9721 acagcccagt gtgcggaaac gcaccatccg ttttttaatc acttcgcccg gttgcaggtc
     9781 gatacggttg tcatcaatca tcatcaacat accgtcgcgt tccagaaccg gacgttccgc
     9841 agcaagatac agcggaacaa tgtcgatatt ttccagccag atgtattgcc agatatcctg
     9901 ctcggtccag ttagagagcg ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt
     9961 gtagttgtgc cacagctccg ggcgctgatt tttcggatcc cagcgatgga agcggtcacg
    10021 gaaagagtag attcgctctt tagcgcggga tttctcttcg tcacggcgcg caccaccgaa
    10081 ggcggcatca aaaccgtatt tgttcagcgc ctgtttcagg ccttcagttt tcataatatc
    10141 ggtatgtttc gcgctgccgt gtacgaatgg attaatcccc atcgccacgc cttccgggtt
    10201 tttatgcacc agcagctcgc agccgtaggc tttcgccgta cgatcgcgga actcatacat
    10261 ctcgcggaat ttccagccgg tatcgacatg cagcaacggg aaaggcagcg tacctggata
    10321 aaacgccttg cgcgccagat gcagcatgac gctggaatct ttaccgatag agtagagcat
    10381 caccggattt gagaattctg cagccacctc gcgaataatg tggatgcttt ccgcctccag
    10441 ttgccgcagg tgggtaagtc gtttttgatc cataaccgtt cctttgcaat accgctattt
    10501 tcttgccatc agatgtttcg actataggga gcgtaagaga acgaatgaaa ttaccaatta
    10561 gaatgagtag ttccttaacg gaataacgat ttggcaaagc taatatcaaa aagtgcttaa
    10621 ggcaccggat ttcgggcgtt taggaagatt tgaaattgtt ttagcgcagc ggcagtttca
    10681 tactatggcg gtaaaaaaat ttgcatggta tttaaggact cactatgttt tccgcattgc
    10741 gccaccgtac cgctgccctg gcgctcggcg tatgctttat tctccccgta cacgcctcgt
    10801 cacctaaacc tggcgatttt gctaataccc aggcacgaca tattgctact ttctttccgg
    10861 gacgcatgac cggaactcct gcagaaatgt tatctgccga ttatattcgt caacagtttc
    10921 agcaaatggg ttatcgcagt gatattcgga catttaatag tcggtatatt tataccgccc
    10981 gcgataatcg caagagctgg cataacgtga cgggaagtac ggtgattgcc gctcatgaag
    11041 gcaaagcgcc gcagcagatc atcattatgg cgcatctgga tacttacgcc ccgctgagcg
    11101 atgctgacgc cgatgccaat ctcggcgggc taacgttaca aggaatggat gataacgccg
    11161 caggtttagg tgtcatgctg gaactggctg aacgcctgaa aaatacgcct accgagtatg
    11221 gtattcgatt tgtggcgacc agcggcgaag aggaaggaaa attaggcgct gagaatttac
    11281 tcaagcggat gagtgacacc gaaaagaaaa atacgctgct ggtgattaat ctcgataact
    11341 taattgttgg cgataagctg tatttcaaca gtggcgtaaa aacacctgaa gcagtaagga
    11401 aattaacgcg cgaccgggcg ctggcaattg cccgcagcca tggaattgcc gccaccacaa
    11461 atccaggttt gaataaaaac tatccgaaag gcaccgggtg ttgtaatgac gcagaaatat
    11521 tcgacaaagc cggtattgct gtactttcgg tggaagcgac taactggaat cttggcaata
    11581 aggatggtta tcagcaacgc gcaaaaacag ccgcattccc tgcgggaaat agctggcatg
    11641 acgtaagact ggataatcaa cagcatattg ataaggcact tcctggaaga atagaacgtc
    11701 gctgccgtga tgttatgcgg ataatgctac cgttggtgaa ggagctggcg aaggcatctt
    11761 aatgggtttg aaatgattac tcccttgcct gcaggaaatc aatgccacca gaaacagagt
    11821 ataaaaaagg gtgcaggaga ccaaaccatc acaattcagt acgcggggtt ttaccccgcg
    11881 tggtggctac ttcttcgatt cgtaagcgag aactgcctta aactccatac cacgttctct
    11941 gatactcaac tcacacaacg agtcgcctac catcaacgtg aatcccagta ctgtacaaca
    12001 cagtacgatg attgccacaa gggcatattt tgtcagcatg tcttgcctcc ctgcaaagaa
    12061 gagactaaca tccgaattgc ttaggttcga acgtaggcct caggttgata gaaatatcgc
    12121 ctggggcttt tgtccatctg gaacctcgcg aatgcttaac gccagacagc ctcaagcacc
    12181 caatgccatt ctatacctgt taattctccc tgcgacgttc tcgtttaaat gctggaatgc
    12241 tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag gcgtttaatt tatccggcaa
    12301 tatttaccct tcgtgtaatc cacattccct ttttaagcca aagaaacgcg tttcttcttc
    12361 cgccatgccg ggttcccatt tacgggttgt atgggtatcg cccaccgata aatatccttc
    12421 atcccataat gggtgatatt tcaggccatg tttttgcagg tactgataaa tagttcggtt
    12481 atcccagtcg ataatcggca gcactttaaa tacgccacgc tgaattgcca gcaccggtaa
    12541 attggcgcgg ctgccggatt gttcacggcg caggccagca aaccaggttt gcacattcag
    12601 ttctttcagc gcccggttca tcggttcgac tttgttgatg tcattgtact tttcaatgcc
    12661 ttcaacgccc tgctcccaca gtttgccgta gcgtgcttcc tgccaggcgg cgctttcggt
    12721 agcacggtac actttcaggt tgagcttgag tttatccgtt aactcgtcaa taaagcggta
    12781 ggtttccggg aacagatagc cggtatcggt gaggatcact ggaatatccg ggcgaatctg
    12841 attcaccaga tgcagactca ccgccgcctg aatgccaaag ctggaagaaa gtacatattc
    12901 accgggcaga ttatccagcg cccaggctac gcggccttca gcgtccagtt tttccagttg
    12961 ggcgttagtt tccgccagcg ccagaatccg gtctaccttt ggcagttcgt tcagggcgtt
    13021 tagatcgagt ttggacataa gttcctcact gtttgccttg cctgatgcaa cgcttacaca
    13081 tcttatcagg ccaacaagac cgggctgatg gttaatccca caaatcgcgc gccggatcga
    13141 gcaccgggcg aatgatgccc gcacgcaccg taaagtcgcc gaagccttca cccgcttcgc
    13201 gctctttcgc ccagcgccct atcagttcat caagcgacgc caggatttcc ggctcggtga
    13261 tgttttcttt atacatccgt gggatacgtg tcccaatgcg gttgccgcca agatgcaggt
    13321 tgtagcgacc cggcgctttc cccaccagac ccacttccgc cagcatcgcg cgaccacaac
    13381 cgttcgggca gcctgttaca cgcatcacga tatgctcatc gctgacacca tgtttcgcca
    13441 ttaaattatc gatgttgtcg ataaaagacg gcaggaaacg ttccgcttcc gccatcgcca
    13501 gcgggcaagt cgggaatgac acgcaggcca ttgagttttc acgctgcggc gtgacggcat
    13561 tcattaaccc gctttctttg gcgatcttct cgatcttcgc tttctcgctt tccggcactc
    13621 cggcgatgat cagattctgg ttcgccgtaa tgcggaaatc acctttgtgg atcttcgcga
    13681 tctccagcaa gccggttttc agcggacgtc ccggataatc aagaatgcga ccattttcga
    13741 taaacagcgt caggtgccag ttatcatcaa tgcccttaac ccagccaata cgatcgccgc
    13801 gtccggtaaa ctcatacggg cgaatcggct cgaatttgat ccccgcgcga cgttcaactt
    13861 ccgctttaaa cgtctcaacc cctacgcgct ccagcgtgta tttggttttg gcatttttac
    13921 gatcggttcg gttaccccag tcacgctgag ttgtcacgac ggcttccgcc accgccagcg
    13981 tatgctccag cggcagatag ccaaactcac tcgccgtgcg ggcgtaggtt ttcttgttgc
    14041 cgtgttcaat ggaaagccca ccgcccacca gcaggttaaa gcccaccagc ttgccgtttt
    14101 cggcgatcgc cacgaagttc atgtcgttgg cgtgcagatc gatatcgttc tgcggcggga
    14161 tcactaccgt ggttttgaac ttacgcggca ggtaggtctg accaaggatc ggctcttcat
    14221 cagtagtggc gactttttcc tgatcgagcc agatctccgc atacgcgcgg gtacgaggca
    14281 gcagatgttc agagattttc ttcgcccatt catatgcttc cgcgtgcagt tgcgactcgt
    14341 aagggttcga ggtgcagagt acgttacggt tcatgtcgtt ggcggtcgcc agcgcatcaa
    14401 gaccgactga gtgcagcatc tgatgcaccg gtttgacgtt ctttttcaga atgccgtgga
    14461 actggaatgt ctggcggttg gtcaggcgaa tgctgccata gatggtgttt tcaccggcaa
    14521 atttgtcgat cgcctgccac tgtttagtgg taatcacccc acccggcaga cgacagcgaa
    14581 gcagcatcgc gtggcgcggc tccagcttct gttcagcacg ttcggcgcga atgtcgcggt
    14641 catcctgctg atacatgccg tggaagcgga tcagcaggaa gttatcgccc ttaaaaccgc
    14701 ctgtcagacc gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg ctttcaagct
    14761 tcatgcgctc ggcgtctgtc agttttcctt cgaccactaa aggccctgga tgtttttcgc
    14821 tcattagtag acatctcgct gataacggcg ctctacgcgc agctcactta aaaattcatc
    14881 cgccgcttcg gtgtccatgc caccaaattc ggcaatcact tccagaagtg cctgctcaac
    14941 gtctttcgcc atgcgattag cgtcgccgca gacataaatg tgggcaccgt cattgatcca
    15001 gcgccacagc tctgcgccct gttcgcgcag tttgtcttgt acgtaaattt tttctttttg
    15061 atcgcgcgac caggcgagat cgatgcgcgt cagcacgcct tctttgacgt aacgctgcca
    15121 ttccacctgg tagaggaaat cttccgtaaa gtgtgggttg ccaaagaata gccagttttt
    15181 acccggcgct tcgtcggcag cacgctgctg cataaaggca cggaacggcg cgatgccggt
    15241 gccagggcca atcatgatca ccggggtttc cggattggtt ggcaggcgga agttgtcgtt
    15301 atgttcgata aagacgcgga cttcaccctc ttcttccaca cggtccgcga ggaagctgga
    15361 ggcaccaccg gcacgggcac ggccttccac gtcgtaacgc accacaccaa cggtaacgtg
    15421 cacttcgttc tcgacttccg cctgcgagga ggcgatggaa tacaggcgcg gggtcagcgg
    15481 gcgcagcaga ttaatcagcg cttcggcatc cagttgcgcc ggagagaaac gcaccatgtc
    15541 gacaatcggc gtcgtcgcgg cgtaatgctg taatttcgct ttatcgccca ccagcggcag
    15601 cagcgtttcg ctgcgggtaa gcgtggcata gttctcaaca atgttggcgg tgttgacggt
    15661 cagttcgaag tgccactgta gcgcttcgtt cagaggcaac gttttgccct cgacggtgac
    15721 aggttcatcg cctttcagcc acagcagttc gacaagttct ttcaccagtg ccggatcgtt
    15781 ctgataccag acgcccagcg cgtcacccgg ctggtaacgc aggccagagt cacctaagtc
    15841 aatttcaatg tgacgaacgt ctttttcaga gttacgcccg gtaattttct ggttaacaga
    15901 gaggctcgcc accagtggcg cgtctttgct atacgggctg gtgtggattt catttaccgt
    15961 gccagtagcg acggattgcg aaggtgccgc gacaggcgca cgcgatttaa gcgcatcaac
    16021 cacgcgggcg cgccactcgc tggcagcagc ctggtattca acgtcggcat cgacacggtc
    16081 gagcaggcgt tcaccgccca gttccgccag cttactgtca aaatctttcc cggactggca
    16141 gaaaaattca taagagctat cgccgaggct aaacacggca aacgcggtgt tttccagctt
    16201 tggcgctttt ttggagaaca ggaacttatg cagcgcgacg gcttcttccg gcggttcccc
    16261 ttccccttgc gttgacgtca ccacgataag cagtttttcg ctggcgattt gtttgaattt
    16321 atagtcaccc gcgttcacca gcttaacgtt cagttttgcc gctaataaat catcgcgtaa
    16381 tgcttcagca acccggcgcg cattgccggt ttgcgaggcg gagataatag ttatacccgg
    16441 catttctgcg gcgggcgctg gcgtcgctgc aagcgcagca ggctgctggt tgagcacgcc
    16501 ccagaaatag ccagaaaccc aggcaagctg ggtaggagtt aaatcggtcg tggccgcctg
    16561 aaggcgtgcc agttgctccg ggttcaacgg aagcaacgcg gaaggtggga cctgtgtcgt
    16621 catgcgttgt tatgttccag taagcaaagc tgtttctgcg ccctgtcagc gcccataaaa
    16681 cagaagagaa ggtaaggtta acggggtaaa cggtgtggat taaagacggg atagcgataa
    16741 ctaataacca aatcgactaa cctgttttag caatagtctt taacaacaaa atagattaac
    16801 caacccaatg aaaaacaaat gaatattgcc catcaataag ataaatcagc gattttgcgc
    16861 aacaaagtcg ttttagataa tgcgaaaaaa cagcctttcc ggtactctac ggcggtttta
    16921 tcgtcatgta gagaaattat gatgtccacc acgttattta aagatttcac cttcgaagcc
    16981 gctcaccgct tgccgcacgt cccacaaggg cataaatgtg gtcgcctgca cgggcattcc
    17041 tttatggtgc gactggaaat taccggggaa gtcgatccgc atacgggctg gattatcgat
    17101 ttcgctgaac tgaaagcggc gtttaaacca acttatgagc gcctcgatca ccattatctc
    17161 aatgatattc caggtctgga aaacccaacc agcgaagttt tagcaaaatg gatttgggat
    17221 caggttaaac ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac ctgcaccgca
    17281 ggttgtatct atcgcggcga atgataagag tgtgtcggcg gtcaatttcc cttaagtaac
    17341 gctatgttag ggtgttgtgt tctggatatc tggggcatga catggaagac gactgcgaca
    17401 ttattattat cggtgccggt attgctggca ccgcttgcgc gttacgctgc acgcgagcgg
    17461 gtttatccgt tttgttactg gaacgagcgg agattcccgg cagcaaaaat ctttccggcg
    17521 ggcggctata cacccatgcg ctcgcggaac tcctcccgca atttcacctg accgcgcctc
    17581 ttgaacgacg catcactcac gaaagtcttt ctctgttaac gccagatggc gcaacgacgt
    17641 tttccagctt actgcccggc ggtgaatcct ggagtgtatt acgcgcacga ttcgatccgt
    17701 ggctggttgc cgaagccgaa aaagaaggtg tcgaatgcat ccccggtgcg acggtagatg
    17761 cactgtatga agaaaacggc agggtgtgtg gtgtcatttg tggtgacgat attcttcgcg
    17821 cccgttatgt ggtgctggca gaaggtgcca acagcgtcct ggcggaacgc cacgggttag
    17881 tgactcgtcc tgctggcgaa gcgatggcat tggggatcaa agaagtgctg acgctggaac
    17941 catccgctat tgaagaacgt tttcatctgg agaataacga aggcgcagcg ttgctgttca
    18001 gcggcggaat ctgtgatgac ttacccggcg gcgcatttct ttatactaat caacaaacgc
    18061 tctcgttagg gattgtttgc ccgctctcct cccttacgca aagtcgtgtt ccggcaagcg
    18121 agctgctggc tcgctttaaa acgcatccgg cagtgcgccc gcttatcaaa aacacggaat
    18181 cactggagta tggtgcgcat ctggtgccag aaggtggcct gcgcagtatg ccggtgcaat
    18241 acgccggtaa cggctggctg ctggtgggcg atgcgttgcg cagttgcgtc aataccggaa
    18301 tttccgtgcg cggtatggat atggcgctaa ctggcgcgca ggcggcggca caaacgctga
    18361 taagcgcctg ccagcaccgc gagccgcaaa atctgtttcc gctttatcat cacaacgtag
    18421 agcgcagcct gctgtgggat gttctacagc gttatcagca tgttccggcg cttttgcaac
    18481 gccctggctg gtatcgggtg tggcctgggt taatgcagga tatttcccgt gatttatggg
    18541 atcaggggaa tacacctgtt ccaccgctgc gccaattgct ctggcgtcat ttacgtcgtc
    18601 acggcttgtg gcatctgacg ggcgatgtta tcaggagtct gcgatgtctg tagcccgtaa
    18661 tctctggcgc gctgccgatg cgccgcacat tgttccggct gactccgttg agcgccagac
    18721 ggcacaacgg ttgattaacg cctgtccagc aggtcttttt tcgctcacac ctgaaggtga
    18781 cttacgcgtt gattatcacg gctgcctgga gtgtggcacc tgccgtttgc tgtgcgacga
    18841 atcaacacta caacagtggc gctatccgcc ttccggattc ggcatcacct accgctttgg
    18901 ataaataata aggataattt atgcccctct tacacctgct ccgccagaat ccggtgattg
    18961 ctgccgttaa agataatgcc agcctgcaac tggcaatcga ctctgaatgc cagtttattt
    19021 ccgtgttgta cggcaatatc tgcaccatta gtaatatcgt taaaaagatt aagaacgccg
    19081 ggaaatatgc ttttattcat gttgatttgc tggaaggcgc gtcaaataaa gaagtggtga
    19141 ttcagttttt gaaactggta accgaggcgg acggaattat cagtaccaaa gcctcaatgc
    19201 tgaaagcagc aagagcggaa ggttttttct gtattcatcg actgtttatt gttgattcga
    19261 tttcgtttca caacattgat aagcaagttg cgcaatcgaa tccggattgt attgagatcc
    19321 tgccaggctg catgcctaaa gtgctgggct gggtgacgga gaaaatccgc caaccgctga
    19381 ttgctggcgg gctggtgtgc gatgaagaag atgcgcgtaa tgcgattaac gcgggtgttg
    19441 tggcgctttc caccacgaat actggggtct ggacgttagc gaaaaaattg ctttgacggg
    19501 ataatcgtaa caagttgaat ttgttgtgat ttgtaggctg cacaccacat ccgacattca
    19561 gcgcctgatt cgacgcttga cgcgtcttat caggcctaca agtccccagc ccagaaccat
    19621 aggcaggatc atccggcagc acgttattgg caatctgcgt gaatgtttgt caccagtgcc
    19681 tccagcacca ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa taccgctgca
    19741 ctggcgtcat gattaatcgc caccacaaac ttgctattgc gtacgcccgc cattaacgct
    19801 gctgcgccgg aagcgccaac aacaatacac acctcaggtg ccagcagatg tccggaaata
    19861 ccaatcactt tttcggcatc aacgccaccg ttcatcactc gtgcccgact gtagcccact
    19921 tcggctccca tcttttctgc cagcatagcg atttcctggt tatctgcctc tccgccctgt
    19981 cccaccacca gcacacgtct ggcctcggtt aatgggtcac gggtaacatt tttcaggttt
    20041 tcgacgctga taagccagtc tggaagcgcg ccggggacaa tgttcagttg ctgcatccca
    20101 gagggcaacg tggcgttttt atctgctccc gcctgacgtg ccagcgaaag gcatagcggg
    20161 cgtttttctg tttgcagcgt cgccgttagc gcatttcccc agtgggattt gcgcacgctt
    20221 acggttggaa tatctaacga agtcacctgg cagatgcttg caccatgtaa acgccaggcc
    20281 agccgggtcg acagttcatc accaaacgtt cccggcggaa agagaacaac gtccgctggc
    20341 gttcgctgcc actgttccac cagcgcatcc agcacctgtt ccgccactac gggctgaggt
    20401 tcaatctgcc agtgtgccag ggtacaaccg ctgaaatcct gtgccgccag ccagcttgct
    20461 attgcggcat tctcctgatt tattgtcaca attgcgatat tcatggctgc atcctttggc
    20521 gtaaatagtt ctgccatagc ttttgcgctt tttctgctac cgtttgccca tcaatcagcg
    20581 tcgctccgcg tcgttgctct gctcttgcca gttgcagaca ctgcatcgct ggcaattcgg
    20641 cagcaacagt tttacggata atttctgctt tccccgcagc cattcgctgg cgcattcccg
    20701 gtactggcaa ggccacttca ccacactggc gcacggcaat caccgcaggc aagcgaaccc
    20761 ggcaacagcg caacccatgt tcagtacgct gttcgagggt gataaacggc gcgtcgagag
    20821 tgaaacgctc cacctgggtg aaacagggcc agcccagcat ttccgccagc aaaaatggcg
    20881 tttgttcatt ctgcccttcg ctgctttggc agccgataat tatcagatcc agcggattct
    20941 gacgctgcca ttcggcaata tggcgagcga caaattccga cgcaaagcgc agatctgccg
    21001 ccgtctccag cactacagct tcctcaaacc caagagccat gagatagcgc agccagtgca
    21061 acgcccgttc atcccccatg ctcaacgcgg ttaaagacat cgacgtaccg ttttttctct
    21121 gcgccaacag cagcgcggcg gcagcctgtt catcagcacc gagtaaactt cgcagtagcg
    21181 aaacatccgg tccgctatta ccctgagccg ccgcctgcca ctctttttcc gccagcattc
    21241 cggcatccgg ctcggcttta aacgctaaca gaatgttcat tcgcacctcc tacatttccc
    21301 gccgccacca gcgggagtgc tttggtttct ggtgcccata gccaggtcac aacaaaacca
    21361 accaacaaca ctgccgccag gagcaataag gtgacttgca ttccccactg cgccagcacc
    21421 cacggcagca ggccagtact taccgccgcg ccaagtcgac tcatggcggt ggcaaaaccg
    21481 acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc caccagatta
    21541 ctgactgccg aaatggtggt gctgaagaga acaaaaagca gcagggttaa tgaactgccg
    21601 gaaggcaaac aggccatcac gaccagcgtt gccgccagca gcaaaaaact tcccagcaaa
    21661 aatttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc cacaattaac
    21721 aacgcattaa gcatcaggct agcggtcaac gcatcttcca gaccaatagt ttgggcgata
    21781 gttggcagcc aggtataaat cacaaaccat gggattacga ggcagacaaa gaagacgctg
    21841 ttaaacgccg tgcgccgcca gtagcgcgaa gagaacaagg ttttgatgtg tttatgggtc
    21901 gccgttgcca cttcatcgcc cagtaaaaca tgcgggccaa aatagcgatg aacgatagcg
    21961 tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gcgattccgg cgtgccccaa
    22021 cgtagcagcg taatcagcaa ggctggtaac gccgctgatg ccagcagcca gcgccaggcc
    22081 tccgggcttt cggaaataaa gtgatgtccg gcaatacttg ccagcacata gcctacggtc
    22141 cacaccacac tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga aaattcagcc
    22201 agcaaggtgt gaccgactga gtaatcgcct cccagaccaa tgccgatcaa aatgcgcaat
    22261 ccaataagat gctctggcgt ggtggcaaaa aattgcaaga acgaagcgag cgtaatcagc
    22321 ataaaactga aggtgaagat tttttgccgc ccgatatggt cggagatcca ccccaaaacc
    22381 aggctgccaa gaaacaaacc aagtaacgcc gagccgccga tcattcccgc cataaacggc
    22441 gtcagttgca tggcgggcgt aagctgaata atggcgtaac caataacgcc gaggacataa
    22501 ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaattc gacagtgaaa acggttaagc
    22561 ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt gtccggtaat
    22621 aagaacggct tctcgcctga gaagccgaaa aggcaacgcc ccgtcatcgc ggaacgctgc
    22681 ctgacacatt atttttcaat cggatagata gtacccgtgt tcataatgcc attaggatcg
    22741 aactgctttt tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag tttgctccag
    22801 tgaacgcgat gtttaccgat accgtggtgg tgcaccatcg aaccgccgag acgaatggtt
    22861 tcttcgcaga tgattttgtt gagcggattg tggtatttgt cgatttcctc ttccggctta
    22921 cagtcaacga cgttgtagtc gtagacgaag tacatgttgg tgccgttctg atagctatga
    22981 gaggagtgac cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcggt acgaatacgg
    23041 ttaataacgc tttcgtagat ttcgtgaatg cagctccagc agccggacac ttcagtggta
    23101 aagcccatgt tgccggtttt gaggatctgc acacgttcgg cagctacttt atccggtccc
    23161 cagttcaggt tgttaaacca ggtttcgatc agcttgctgt ccacgcgctg gcactgcggg
    23221 tagcgcgcca cgatttcagc aatcccttca cccgtcgcct tcgcaatgcg agggttacct
    23281 tcagccataa agatcagcac gcatttgccg tcggcaaaat gggtgaagtg ttgggtgcca
    23341 tcttcagcat catacaaacg agcaatcgac ggacgatacc cttccaccat gatttcacgc
    23401 aggatgttga agccggtttt catgtcttcc agcacatagc catagaagag attgttttcc
    23461 ggcgtaaatt tgaagatttt cactgtgact tcagtgatat agcacagtgc accttcgttg
    23521 ccgatgatga tatgacgaat gtccgggcca gccgcgcgac gtggcacgtt tttaatgcgt
    23581 gtgacggtgc catctgccag caccgcttcc agaccaacga ccatatcctc gattgcgccg
    23641 tagagtgtgg aaaactgccc aatactgcgg gtcgccacca ggccgcccat ctgcgccagc
    23701 ggctttgact gcggagaatg ccccgtggtg taaccttttt cacgcaacgc gttttccagc
    23761 acctccagcg gaacgccaca ttgcgccgtg gcctgcatat tctcaatatc aatattaatg
    23821 atttgattca tggcggagcc gtcgagcacc accgagtttt ccacaacggt ttccagccca
    23881 ccttcggtgg cggaagcacc ggtacgtggc acaccgttaa ttttgtgcgc attcataaaa
    23941 ttcagcacac gggatacttg ttctgtggaa ccgagtttta cgaccgctgc cggaattggc
    24001 aaagtataaa tgccatgaat atccggaaat ttacgaaaac ggtcaatact gttcttcttt
    24061 aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag ctggtcgaca
    24121 atcgctacgc gagataaaga catagtaatt ccttcctgat aataaaaatg gaatagttat
    24181 taataagaga aaacagaaac caccgttatg aattagcgca ctaaataacc gccatcaacc
    24241 actaataaat gtccgttgac ataattcgat gccggacttg cgaggaatac ggctgcaccc
    24301 attaaatcct gagtatcacc ccaacggttt gccggaatat gatcaagaac acgctggtta
    24361 gtttctggat tactacgggt cgccagcgta atatcggttg cataataacc aggagcaata
    24421 ccattcacct gaatattata ttggcctaat tcatcgcaat aggctttggt gaatccggca
    24481 agagcatgtt tagtggcaga ataagcaggt gaccattgac cacctaagta agagaacaat
    24541 gaacagatat taatgatttt accgcttttc tgcgggatca taatttttgc cgcttcgtag
    24601 cttaactcga atgcggcggt caggttcacg tcaatcatcg ggtcccagtc ggcacgacca
    24661 aagtccagca ccttattcag cttacaaata ccggcattgt taaccagaat atcgactgta
    24721 ccgaagcgct cacagcaggc agcgataatc ttctgcggcg cgccttctgc ggtgatatcc
    24781 acctgcataa aatcaacttc gacaccctgt ttttcaatca tttcctttgt ttcgccgtta
    24841 tctttgacga aactaggaat aaagacattt gcgccagctt tggccaacgc catggcaaat
    24901 gcctggccta aaccgctatt cccgccggta acaattgctg ttttaccttt cagggagaaa
    24961 aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa tttccgtaag
    25021 gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct cattatctct
    25081 ggtaactgga gataattttt agcataagga cataactgct aatgtgatcg taatcacagt
    25141 gtgatgttcg tattgtcaaa taatttaaaa aagcagaagc gtgattactc tcacacaaca
    25201 cctctgc
//
LOCUS       DAZFRG010000047        24661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFRG010000047 DAZFRG010000000
VERSION     DAZFRG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.047"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..889
                     /gene="nanC"
                     /locus_tag="ACHKUC_001508"
     CDS             173..889
                     /gene="nanC"
                     /locus_tag="ACHKUC_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO6617790.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            909..2015
                     /gene="nanM"
                     /locus_tag="ACHKUC_001509"
     CDS             909..2015
                     /gene="nanM"
                     /locus_tag="ACHKUC_001509"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6617791.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLITVKDNKVTVQN"
     gene            2080..3060
                     /gene="nanS"
                     /locus_tag="ACHKUC_001510"
     CDS             2080..3060
                     /gene="nanS"
                     /locus_tag="ACHKUC_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO6617792.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGACWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3170..3375
                     /locus_tag="ACHKUC_001511"
                     /pseudo
     CDS             3170..3375
                     /locus_tag="ACHKUC_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(3643..4883)
                     /gene="yjhR"
                     /locus_tag="ACHKUC_001512"
                     /pseudo
     CDS             complement(3643..4883)
                     /gene="yjhR"
                     /locus_tag="ACHKUC_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_176259710.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase YjhR"
     gene            4999..5130
                     /locus_tag="ACHKUC_001513"
     CDS             4999..5130
                     /locus_tag="ACHKUC_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617793.1"
                     /translation="MTQVLIGDIYLRQQCDVFWLGYTISPAYARQGYAIEAITATID"
     gene            5621..5878
                     /gene="topAI"
                     /locus_tag="ACHKUC_001514"
     CDS             5621..5878
                     /gene="topAI"
                     /locus_tag="ACHKUC_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HFO6617794.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            5890..6435
                     /gene="yjhQ"
                     /locus_tag="ACHKUC_001515"
     CDS             5890..6435
                     /gene="yjhQ"
                     /locus_tag="ACHKUC_001515"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6617795.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCADPDETGALT"
     gene            6491..7237
                     /gene="yjhP"
                     /locus_tag="ACHKUC_001516"
     CDS             6491..7237
                     /gene="yjhP"
                     /locus_tag="ACHKUC_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6617796.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQLPATEEIAQACGVSS
                     TSDFLTLPGLVGAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNIAPKRYVTYARECFGWGVFALIAR"
     gene            7406..7624
                     /locus_tag="ACHKUC_001517"
                     /pseudo
     CDS             7406..7624
                     /locus_tag="ACHKUC_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7662..7778
                     /locus_tag="ACHKUC_001518"
                     /pseudo
     CDS             7662..7778
                     /locus_tag="ACHKUC_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            8023..9144
                     /gene="sgcX"
                     /locus_tag="ACHKUC_001519"
     CDS             8023..9144
                     /gene="sgcX"
                     /locus_tag="ACHKUC_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFO6617797.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPYVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTMELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            9141..9419
                     /gene="sgcB"
                     /locus_tag="ACHKUC_001520"
     CDS             9141..9419
                     /gene="sgcB"
                     /locus_tag="ACHKUC_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB SgcB"
                     /protein_id="HFO6617798.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            9431..10744
                     /gene="sgcC"
                     /locus_tag="ACHKUC_001521"
     CDS             9431..10744
                     /gene="sgcC"
                     /locus_tag="ACHKUC_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC SgcC"
                     /protein_id="HFO6617799.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLLPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            10757..11563
                     /gene="sgcQ"
                     /locus_tag="ACHKUC_001522"
     CDS             10757..11563
                     /gene="sgcQ"
                     /locus_tag="ACHKUC_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HFO6617800.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            11694..12125
                     /gene="sgcA"
                     /locus_tag="ACHKUC_001523"
     CDS             11694..12125
                     /gene="sgcA"
                     /locus_tag="ACHKUC_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcA family PTS sugar transporter subunit IIA"
                     /protein_id="HFO6617801.1"
                     /translation="MINDIKWVQAQRKATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLTAVGNASTDDALFALVSG"
     gene            12137..12769
                     /gene="sgcE"
                     /locus_tag="ACHKUC_001524"
     CDS             12137..12769
                     /gene="sgcE"
                     /locus_tag="ACHKUC_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HFO6617802.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            12786..13568
                     /gene="sgcR"
                     /locus_tag="ACHKUC_001525"
     CDS             12786..13568
                     /gene="sgcR"
                     /locus_tag="ACHKUC_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6617803.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IVNQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            13871..14659
                     /gene="yjhI"
                     /locus_tag="ACHKUC_001526"
     CDS             13871..14659
                     /gene="yjhI"
                     /locus_tag="ACHKUC_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6617804.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVDCGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            14664..15569
                     /gene="yjhH"
                     /locus_tag="ACHKUC_001527"
     CDS             14664..15569
                     /gene="yjhH"
                     /locus_tag="ACHKUC_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO6617805.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            15580..17547
                     /gene="yjhG"
                     /locus_tag="ACHKUC_001528"
                     /pseudo
     CDS             15580..17547
                     /gene="yjhG"
                     /locus_tag="ACHKUC_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
     gene            17654..19003
                     /gene="yjhF"
                     /locus_tag="ACHKUC_001529"
     CDS             17654..19003
                     /gene="yjhF"
                     /locus_tag="ACHKUC_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFO6617806.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            19350..20336
                     /gene="yjhU"
                     /locus_tag="ACHKUC_001530"
     CDS             19350..20336
                     /gene="yjhU"
                     /locus_tag="ACHKUC_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6617807.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(20377..21381)
                     /locus_tag="ACHKUC_001531"
     CDS             complement(20377..21381)
                     /locus_tag="ACHKUC_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFO6617808.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVVCVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(21485..22126)
                     /locus_tag="ACHKUC_001532"
     CDS             complement(21485..22126)
                     /locus_tag="ACHKUC_001532"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6617809.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(22157..23272)
                     /gene="mtnA"
                     /locus_tag="ACHKUC_001533"
     CDS             complement(22157..23272)
                     /gene="mtnA"
                     /locus_tag="ACHKUC_001533"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HFO6617810.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(23282..24541)
                     /gene="mtnK"
                     /locus_tag="ACHKUC_001534"
     CDS             complement(23282..24541)
                     /gene="mtnK"
                     /locus_tag="ACHKUC_001534"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HFO6617811.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
BASE COUNT         6335 a         6050 c         6036 g         6240 t
ORIGIN      
        1 aataatgtga aaagagattt ttcacaggag accttataca aaaaaatata aaatacagct
       61 accggttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat aaatgctaat
      121 tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa
      181 ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa tttctcaggc
      241 tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg agactcgact
      301 caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta atacctggaa
      361 taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa
      421 ttacgcgatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa cgcattttag
      481 cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga caaaagatct
      541 taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag acttatccgg
      601 tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc atattaatag
      661 tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaacgatt atcgctatgc
      721 aaaccataag aaatgggcga cggaaaatgc atttgttcta caataccata tgacgcccga
      781 tattacgcca tacatagaat atgactacct tgaccgtcag ggtgtttaca acggcagaga
      841 taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt agacaggaga
      901 cagtcacaat gaataaaaca ataacggcgc ttgctatcat gatggcttca tttgccgcaa
      961 acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga gcaattgata
     1021 acgacactgt ctacattggt ttaggtagcg caggtacggc atggtacaag ctggatacac
     1081 aggccaaaga taaaaaatgg acagcgttag ctgcattccc tggcggacca agagatcaag
     1141 caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc aaaaacagcg
     1201 agggcttgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc aatagttggg
     1261 ttaaattgat gtcgcacgcg ccgatgggca tggcgggcca tgtgactttt gtacacaacg
     1321 gcaaggctta tgttactggt ggtgttaacc agaatatctt caatggctat tttgaagatc
     1381 tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caatgctcac tattttgaca
     1441 aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc tcaacacagc
     1501 aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg gttgtgaata
     1561 aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga acggatgccg
     1621 tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgct cccgtctcat
     1681 caccagatgg cgtagctggc ggttttgcgg ggataagcaa tgattctctt atatttgccg
     1741 gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac tatgcgcatg
     1801 aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg aaatgggata
     1861 aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg aataatagtc
     1921 tattgattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca gttttgatca
     1981 ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg gtaaaggggc
     2041 cctgctatca atttgtcatt tcaattacgc gaaatttata tgaacgcaat aatatcgccc
     2101 gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta tggcgaagga
     2161 ctgccattac cggacaggga agatgcgcct catcccagaa ttaaacaatt agcgagattt
     2221 gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc actgactcac
     2281 tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac gaatcatcaa
     2341 acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact gcccttcatt
     2401 cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc ttttaccgcg
     2461 ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg tcgttgggga
     2521 acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact ggcaaaaaat
     2581 ccgcagaaca aattcctcgg cgcatgctgg atgcaaggcg aatttgactt aatgaccagt
     2641 gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg tagggatcta
     2701 aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg cggcgatacc
     2761 acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg caattatcaa
     2821 aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga aagaggactg
     2881 acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg ttcagcgtac
     2941 cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac tgcagcccgt
     3001 cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg cgaaaggtga
     3061 gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca acaaaaaaca
     3121 aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa ttgccaatat
     3181 aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat tgcttacaga
     3241 ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga aatctacgaa
     3301 catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg tcctgatatg
     3361 ccgttctggc gattataagt cgacggattc tggcaaatag acgatgatga tatgcttaat
     3421 gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt cattcctaaa
     3481 agcacccgcc attaatccca ggcgcattat gcaaaggaaa atgattaggt aaacaataag
     3541 atttataatc aggaaaggcg actcacaata aaacggaaag tcgccttaac attggtaaaa
     3601 tatgcccttt actactcctg tatcatgaac ggctcatcac atttaaagct gacgttgatc
     3661 cagactacca tatattgcct ttatttcatt ttttacgcct tcaccacggt acaccaaaga
     3721 cgtatcgtat cgttgatact tatcttcccg tgtagcactg aaccagttaa aagatccgac
     3781 acacagcagt tcttcatcct caatcacaat tttgctgtga acacgattaa ccaatttcgt
     3841 agcgataccc atcttattga gcttttcaac agcatcattc agcagatgtt gtttctcctg
     3901 acgcttatca tcatcgacat gcgctatatt gcagttttta tccgtcacca cggtaatatc
     3961 aatgccccgg gaacgggcca aagccattga tgccagaaat cccgtttgct ccactttttg
     4021 ccaggataac caaggggaaa taatcgttat tttcttttga gctccggcaa gagtcttgtt
     4081 caaaaattca tcatgctgct ccacaccatg cagcgttgaa atttgtgtat gtgcggaaat
     4141 taaatcctgt cgtttctgaa actcaaattg caacgcatta ttatctgaag aaaagagata
     4201 ttttgccaat aaccctcgcg gcgaaaatgc gggctgcatc tcgataaggt ccatatcgcc
     4261 aaataccaga aaactatctt tagcgcgtga gacagcaaca ttgaggatag tgctgttgct
     4321 atcaagaaac ctgccatctt catgttttga gtagacagga gaaaagagaa caatcgccct
     4381 ttccgccccc tgcaaggagt gcacagtccc cactgtcagt aaaccctgct cgtctttgcc
     4441 attaatttcc agcttacgca atgacatttt gatagcatta acctgcgccg aaaatggcgt
     4501 cacaacacca acaactttgt acagtggttc cccgtaatgg cgctctatat catccttatg
     4561 tgccaccagc catgctgcta ttgtttcagc ttcaagaggg ttatgacggc tccctccatt
     4621 tggcttcatg cctctaccat caatatgtaa atatcccatt gcggggaaaa tcgtcctttt
     4681 tccatccctc ttttaggctg caacttaccg tgatagcaaa gtgagttaca gtagtcgatg
     4741 atgttattga aacagcgacg atgctcatac agatacattc cgcgctcgaa cttgggatca
     4801 tactggtagt gagagttgaa ctgggctacc tgcattacgc tgccagatgc tgcacttttt
     4861 ccagacccat ttattatcga atacggtgac cgcctgagcg gccaccttta atcgtgggca
     4921 gttacacaga attatttaca gggtcttgct gggagttctt acacttgctc gcgatattca
     4981 attggccatt gtcaacaaat gacgcaggtt ttgattggtg atatctattt acgtcagcaa
     5041 tgcgatgtgt tttggctcgg ctataccatc agtcccgctt atgctcgcca gggatatgca
     5101 attgaagcaa ttaccgccac gattgactga ataaaagaga acggtttttc actcatcaaa
     5161 gccggtgtga atccggaaaa taccctttca aaaaaattac ttatcaggat tggcttcaat
     5221 ttttcaagca tcgaagatgg gtgagcatat ttatgttctg gatttaacaa aaggagaata
     5281 atctcccgaa gggaaaagcc gatgaggcca tttcaggcgt acttcaacat gcattcgtca
     5341 gtgtcatctg ccttttccag taaatgggtt ggcattcaat ttccaaagta catcacactt
     5401 tttctactga aacatatccc tcctataact acgtgttaat attcgctccg taaatacctt
     5461 tcaactcaac ccgaggcttt gatgctggag aatgtaatca tccgataatc agttccccga
     5521 ccttttcagg ccggactgat tatcaatgcg ccgaaatcga atgcggacac cgcggtgtgt
     5581 ttgcacgttt tgcacatgat gattaaatag gttcttcagt atgaatttat cccgtcagga
     5641 acaacatacc ttacacgttc tcgctaaagg tagacgtatt gcgcacgtcc gcgattcttc
     5701 aggccgcgtc acttccgttg aatgctacag ccgcgaaggg ctgttgctga ccgactgcac
     5761 gctcgccgtc ttcaaaaaac tcaaaaccaa aaaacttatc aagtccgtca atggccagcc
     5821 ctatcgaatc aacaccaccg agctgaataa agttcgcgct cagctcgata atcgctaagg
     5881 agagtttcta tgacagtaca ccacttcact tttcatatca cagataaaag tgatgccagt
     5941 gatatccggg aagtcgaaac ccgcgcattt ggcttcggca aagaagccga tttagtggcc
     6001 tctttacttg aggatgaaag cgcgcgcccc gcgttatcgc tgctagcccg gtatgaaggc
     6061 aaagccgtcg ggcatatttt atttacccgg gcaaccttca aaggagaaat ggattcaccg
     6121 ttgatgcaca tcctcgctcc cctggcggtc ataccggaat accaagggat gggcgtggga
     6181 ggacggttga tacgcactgg catcgagcac ctgaggttga tgggctgcca gacggttttt
     6241 gttctcggcc acgccaccta ttacccccga catgggttcg aaccctgtgc cggagacaaa
     6301 ggctatccgg caccttatcc catcccggag gagcacaaag cctgctggat gatgcaatca
     6361 ctcaccgctc agcccatgac cctgaccggc cacatccggt gcgcagaccc tgatgaaacc
     6421 ggagcactga cgtgaataac aatcttgttg tgtccttttt aaggcttaac ccaataacag
     6481 gaattaaata atggatatcc cacgtatttt taccatcagc gaaagtgaac accgcatcca
     6541 taacccgttc accgaagaga agtacgccac gctgggtcgc gtgctgcgca tgaagccggg
     6601 cacccgcatt cttgacctcg gcagcggctc gggcgagatg ctctgcacct gggccaggga
     6661 tcatggcatt acggggactg gcatcgacat gagttcgctc ttcaccgcgc aggcaaagcg
     6721 tcgcgcggaa gaactcggcg tcagcgaacg cgtacatttt attcataacg atgcggccgg
     6781 ttacgtcgca aatgaaaaat gtgacgtggc ggcctgcgtc ggcgcgacat ggattgccgg
     6841 tgggtttgcc ggggcggagg agctgctagc gcaaagcctg aaaccgggcg ggatcatgct
     6901 tatcggcgaa ccctactggc gtcagctacc agcaacagaa gaaatagccc aggcctgcgg
     6961 cgtcagctcg acaagcgatt tcctgacgct gccaggactt gtcggagcgt tcgacgatct
     7021 cggctatgac gtggtggaaa tggtactggc agaccaggaa ggctgggacc ggtatgaagc
     7081 cgcaaaatgg ctgaccatgc gccgctggct ggaagcgaat cctgacgatg acttcgccgc
     7141 cgaagtcagg gccgaactaa acatcgcgcc gaaacgctac gtgacctacg cgcgggaatg
     7201 ctttggctgg ggcgtgttcg cactcatcgc ccggtaacaa caggttcccg gtatttctgg
     7261 ctggaaatat cgaggcttta cctttgcttt gatctgatgt atcacctgca caatacatat
     7321 tcagtatgtt tattaatcaa gatgtcacca taaggagcat catatgtcac aacatgaaca
     7381 gttctgtcag gcttgcggaa tgccgatgtc ggcaccagat gcacacagtg ccagtgatca
     7441 atattgtgcg taatgcagcg acagcaacgg caatctcaaa ccctgggaag aagcggtatc
     7501 aggtttagcc agcttccttg attcctggca aaaagtcggc ccggaggaag cacataagcg
     7561 tgcaaaacgt tatctgacgg caatgccagc ctgggctcat aaagcggaga gtgatttgag
     7621 ctgattatct atactccctc tgaatctatt caggagatcc gatgttttct tacattatgc
     7681 tgggaaccaa cgacctgcca cgcgccatta cattctaaga tccgctgatg gagatgctgg
     7741 ggtatccacg tgcaggacgt aatgaagagg gagcgtcctg caacaaagtg gtgctataac
     7801 agttaccgcg ttttattcat cctcattatt tcaaccataa tcactggtct ctccagcccg
     7861 taactttcac aaacgttgag cgcttttatt ccactcggct cacataattt tttatttgtt
     7921 cagctttgcg cgttttcacg tgaacgttct cacaattatt cgaccagtct gcgcctataa
     7981 aacgctcaac gagtgattgt tttacggtct acggagatag ctatgtcatt ttctgtgcag
     8041 gaaaccctgt tctcgctttt gcagcacaat gcgatttcag gacacgaaaa cgctgtcgct
     8101 gacgtcatgc tgtgcgaatt caggcgtcag gcaaaagagg tctggcgaga caggcttggg
     8161 aatgtcgtcg cgcgctacgg tagtgataaa cccgatgcgc tgcgactgat gatttttgcg
     8221 catatggatg aagtcggttt tatggtgcgc aaaatagagc cgtctggatt tttacgcttt
     8281 gaacgcgtag gcggtcctgc gcaggtcact atggctggtt ccatcgtcac cctcaccggg
     8341 gacaaagggc cagtcatggg gtgtatcggc attaagtcct accactttgc caaaggcgac
     8401 gagcgcacgc agtcacctta tgtcgacaaa ctgtggattg atattggtgc caaagacaaa
     8461 gacgacgcta tacggatggg cattcaggtc ggtacgcctg tcactctgta taacccgccg
     8521 caactcctgg caaacgatct ggtgtgcagt aaagcactag acgatcgtct aggctgtact
     8581 gccctgctcg gtgtagcgga tgctatcagt actatggagc ttgatatcgc cgtttatctg
     8641 gtggcttcgg tacaggaaga atttaatatc cgcggcattg ttcccgtatt acgccgtgta
     8701 aaacctgacc tggcgattgg tattgatatc actccatcgt gtgacacccc tgatttacac
     8761 gattattccg aggtcaggat taatcagggc gttgggatca cctgcctgaa ctaccatggt
     8821 cggggaacgc tggccggatt aatcacgcct cctcgcctga tacggatgtt ggaacagacg
     8881 gctcttgaac acaacattcc ggtgcagcga gaagtggctc ccggcgtgat aacagaaacc
     8941 ggttatatcc aggttgagca ggatggtatt ccctgcgcca gtctctctat tccttgtcgc
     9001 tatacccatt ctccggcgga ggttgccagc ctgcgtgatt tgactgattg catccgccta
     9061 ttgaccgctc tggcaggtat gtcagcagca catttccccg ttgagcctga ttcaggcact
     9121 acacaagagg cacatccatt atgaaaaaga tccttgtggc atgcggtacc ggcatgtcga
     9181 cttcaaccat gattgcacac aagctgcaag agttcctgac tgaacagggc atttcagcca
     9241 ctaccgccca atgctgtctg aatgagatcc ctcttaactg taacggtatg gaccttattg
     9301 taacctccat gcgaaccaac agtgattacg gcatccctac gcttaatggc gccgccctgc
     9361 tcacagggat caacgacgac gcgttaaaac aacaaatcaa ggctctgtta actcaataaa
     9421 aggaggcatt atgtttgatt acatcctgtc tctcggcggt accgttttcg tccctatcat
     9481 catgatcgtg attggcctta tcttccgcat cccctggctg caggcgatta aggcaggggt
     9541 aacggttggg attggttttg tcggtatggg actggtgatc gtcatggcga tcgacagcct
     9601 cagtccccct atcaaagtga tgattgagcg tttcggccta gccctgcatg tctttgacgt
     9661 tggcgcaggc cctgcctccg gcgttggtta tgcgacagcc attggcgcca tgattatccc
     9721 agttattttt cttctcaatg tcgccatgct ggtcacccgt ctgaccaaaa cgatgaacgt
     9781 cgatatttac aattactggc actacgccat tacgggtacg gtggtccagc ttatgaccgg
     9841 tagcctgatt tatggtgtgc tgggggcaat ctgccatgcg gcgctatcgt taaaaatggc
     9901 cgactggacg gcaaaacggg tacagaacat cgtcgggctg gaaggtattt cgataccgca
     9961 aggctacggt tccagttcag taccgctttt cgttctactt gatgctatct atgaaaaaat
    10021 ccctttcatg aagggacgca atatcgatgc tcaggagatc cagaaacgct acggcatggt
    10081 cggcgatccg gtgattatcg gcgtagtgct cggtctgatt tttggcctag cggctggtga
    10141 aggctttaaa ggttgcgcca gtctgatgat taccgttgcg gcaatcatgg tgttgttccc
    10201 gcgcatgatc cgcctgattg ttgagggcct gctgcccatc tccgacggtg caagaaagtt
    10261 cttccagaaa tatttcaaag gacgtgaagt gtatatcggt ctggatactg ctgtgacgct
    10321 ggggcatcca accaccattg ctgtcggtct gctactgata cccattatgt taattctggc
    10381 cagcatttta ccgggcaaca aagtgttacc cctcgcagac ctgccggtag caccgttctt
    10441 tatctgtatg gctacggtta tccaccgcgg agacctggtc aggacgttga taagcggggt
    10501 catcgtgatg attaccgtcc tgctgatcgc cacccagttc gcgccttact tcactgaaat
    10561 ggcgctcaaa ggcggtttca gttttgccgg tgaaagtgcg caaatctcag cgttgtccgt
    10621 gggcaatatg ttcggttggt cgatttctga attgatgtcg cttgggatta ttggcgttgt
    10681 cgtggcggtc ggtattgtcg ccagcgtcgt gctgttctta cgtaaacgtg aattatcgga
    10741 ataaggagta ctttcaatga gttggctgaa agaggttatt gggacggaaa aagcggtcat
    10801 cgccatgtgc catctacgtg cattgcccgg cgacccgagt tttgatgcgc agttggggat
    10861 gaactgggtt atcgacaaag cctgggacga tctgatggcg ctccaaaatg gtggggtgga
    10921 tgcagtcatg ttctccaacg agtttagtct tccctatctc acgaaggtgc gtccggaaac
    10981 cactgcggca atggcacgaa tcatcgggca attaatgagc gatatccgca tcccgtttgg
    11041 cgtgaatgtt ctgtgggatc cggtcgcctc attcgacctg gcgatggcta ccggcgctaa
    11101 gtttatccgc gagatattta ccggtgccta tgccagcgac tttggcgtct gggacactaa
    11161 cgtcggtgag accatccgtc atcaacaccg gattggtgca ggcgaagtga aaaccttgtt
    11221 caatatcgtc ccagaggccg ccgtgtatct gggcaatcgc gatatctgtt ctattgctaa
    11281 atcgacagta tttaacaacc atcctgatgc gctctgtgtt tctggcctga ccgccgggac
    11341 gcgtaccgac agcgcgctgt taaaacgggt caaagaaacg gtgcctgaca ccgtcgtgtt
    11401 ggccaacacg ggcgtctgcc tggaaaacgt ggaggaacaa ctcagtatcg ccgatggctg
    11461 tgtgaccgca accaccttta aaaaggacgg cgtttttgcc aatttcgttg accaggcgcg
    11521 agtcagtcag tttatggaga aagtgcatca tatacgccga taagatgggg ataagcagag
    11581 cgcttatccc cggcaaagag caataaccct tctgcgatcc ctgccgcctt cgtcaaaagt
    11641 atcgacgggg atcgctacca gattaacccg aatcggcatg gagtcaacga cggatgatta
    11701 acgatattaa atgggtacag gcacaacgta aagccaccga ctggcgtcag gccgtggaaa
    11761 tcgccacccg cccgctggta gcctatggtg ccgcgcagcc atgctacgtt aatgggatca
    11821 tcgaaaatac ccttaactgg gggccttatt atctgatagc cccaggaatc gccttgcctc
    11881 acgcccgacc agagcaaggc gcaaactata atcaggtcag tatcaccacg ctacgcaccc
    11941 cggtcgcctt tggtaacgag gagtgcgacc ccgtctggct gcttctctgc gtcagcgcca
    12001 cggatgccaa cgcacatatt ttgaccattc agcgcatcag ccagtttatt gattcgccac
    12061 aacgcttaac ggcagtggga aatgccagca cagacgatgc actgtttgcg ctggtatcag
    12121 gttaacggag gcgcttatga tcctgcaccc ttctctcgcc agcgcaaatc ctctgcacta
    12181 tggtcgtgag ctgacggcat tggacaatct ggattttggg agcctgcatc tggatattga
    12241 ggacagcagc ttcattaaca acattacgtt tgggatgaaa acggtgcagg ccgtcgccag
    12301 acagacgcct catcccttgt cctttcattt catgcttgcc cggccacagc gctggtttaa
    12361 cgcgctggct gaaatcagac ccgcgtggat cttcgttcat gcagaaacgc tggactatcc
    12421 ttcggaaacg ttaactgaga tccgccatac cggcgcacga gccggactgg tatttaatcc
    12481 ggcaaccccg atcgacgcct ggcgatatct ggcgtctgag ctggacggag tgatggtgat
    12541 gaccagtgag cccgatgggc agggacagcg gtttattccc tccatgtgcg agaaaatcca
    12601 aaaagtacgc acggcttttc ctcagaccga gtgctgggcc gacggtggaa ttacgctggc
    12661 agctgcacag caactggcag cagctggggc gcaacatatg gtgattgggc gcgccctgtt
    12721 ctcttcatct gactatcgcg cgaccttagc tcaattcgcc accctgtaag aggagattgt
    12781 ttctgatgag ccaacagaga cctgaccgta tcaaacagat gctgcactac ctgtggcaac
    12841 atcgtcacct ctccacacag caggccatgg agctttttgg ctatgcggaa gcgactgtcc
    12901 gtcgtgattt ccagtatatt gtcaaccagt atccaggcat gatccgcggt cacggttgcc
    12961 tggacttcga cgacagtaca gacgacaaag agtacgtctt cgacgtgaaa cgtacattgc
    13021 aatctgtggc gaaaagagaa atagctgccc ttgcccgaac aatgatcaaa gatggcgact
    13081 gcttttttct cgactccggc tcaacctgcc tcgaactggc gaaatgcctg gccgatgcgc
    13141 gggtaaaggt catctgcaac gacattaaaa tcgccaatga actgggctgt tttccccatg
    13201 tggaaagtta cattatcgga ggactgattc gcccaggtta tttttcggta ggagagagtc
    13261 tggcgctgga gatgatcaat gccttttccg tagaacgtgc gtttatctcc tgcgatgcgc
    13321 tgtcgctgga gacggggatc accaatgcca ccatgtttga ggtcggggtg aaaacacgga
    13381 ttattcaacg ctcgcgtgaa gttattctca tggccgatca ctcaaaattt gatgccgttg
    13441 agccgcatgc tgtagcaaca ctatcatgta ttaaaacgat catcagtgac agcgggttac
    13501 ctgagactat tgcacagcgt tatcagcgag ccggatgtca gttgttttta cctcattcaa
    13561 taaagtgata agtaagttca ttcgagaggg atttcaagca aaaataatca atggcaccca
    13621 atagaaaata ttggcgatgc gctcgaacga ataaagaagc tctaggcgca atccacacac
    13681 tacgatgttg caacaacacg ccatctactt tttattctca ttcactaaat gtggctgttc
    13741 tggattcatt attcaaagtg tgtacaagat cacatttaat cacatcatta cggttcagca
    13801 tgctgaacaa agcatatttt ccactatgta atgccgatac catttattcc atgagcaagg
    13861 aggagccatt atggttcgca aagggtgtaa ttcattagtc cgtgctgaaa aaatattaac
    13921 ccacattgca tgggttggta tggcaagtta tatggagtta ttaaataaat tccagtaccc
    13981 caaaagcagc ctgcttaatt tattgaatgt aatggtggat tgtggtttct taattaaaaa
    14041 caaaaacggc tactattcac tgggaataaa aaactacgaa ttgggttgtc aggcgttaca
    14101 ccgtcaaaat atatttgagg tgactaagcg gccaatgcag gagttgtcgc tgaaaagtgg
    14161 actggtttgt catcttgggg cgatggagag catttcggct atctatctcg acaagatcga
    14221 aagtccggac tctgtaccga cgagcaaaag ctggattggc aaaaaacttg agttgcatat
    14281 taccgcgttg ggtaaggcat tattagcctg gaagacacga gaagaactgg attatttttt
    14341 agaagcactc acgttaactc cacatacgcg caatacattt accgataaaa aattgtttct
    14401 ggaagaatta caaaaaacac gccttcgggg atgggccata gacaacgaag aatcaaccta
    14461 tggggcggta tgtttaagta tgccggtatt caatatgtat aacagagtta actatgcaat
    14521 ctcactttct ggcgatccgg tagtttattc aggaaataag atagacagct atctggaatt
    14581 gctccggaaa tgcgctgagc aaatatcata tgggctggga tacagaaacg aaaatgagca
    14641 cttacgaaaa ggaaactgag gtaatgaaaa aattcagcgg cattattcca ccggtatcca
    14701 gcacgtttca tcgtgacgga acccttgata aaaaggcaat gcgcgaagtt gccgacttcc
    14761 tgattaataa aggggtcgac gggctgtttt atctgggtac cggtggtgaa tttagccaaa
    14821 tgaatacagc ccagcgcatg gcactcgccg aagaagctgt aaccattgtc gacgggcgag
    14881 tgccggtatt gattggcgtc ggttcccctt ccactgacga agcggtcaaa ctggcgcagc
    14941 atgcgcaagc ctacggcgct gatggtatcg tcgccatcaa cccctactac tggaaagtcg
    15001 caccacgaaa tcttgacgac tattaccagc agatcgcccg tagcgtcacc ctaccggtga
    15061 tcctgtacaa ctttccggat ctgacgggtc aggacttaac cccggaaacc gtgacgcgtc
    15121 tggctctgca aaacgagaat atcgttggca tcaaagacac catcgacagc gttggtcact
    15181 tgcgtacgat gatcaacaca gttaagtcgg tacgcccgtc gttttcggta ttctgcggtt
    15241 acgatgatca tttgctgaat acgatgctgc tgggcggcga cggtgcgata accgccagcg
    15301 ctaactttgc tccggaactc tccgtcggca tctaccgcgc ctggcgtgaa ggcgatctgg
    15361 cgaccgctgc gacgctgaat aaaaaactac tacaactgcc cgctatttac gccctcgaaa
    15421 caccgtttgt ctcactgatc aaatacagca tgcagtgtgt cgggctgcct gtagagacat
    15481 attgcttacc accgattctt gaagcatctg aagaagcaaa agataaagtc cacgtgctgc
    15541 ttaccgcgca gggcatttta ccagtctgag gagaaactca tgtctgttcg caatattttt
    15601 gctgacgaga gccacgatat ttacaccgtc agaacgcacg ccgatggccc ggacggcgaa
    15661 ctcccattaa ccgcagagat gcttatcaac cgcccgagcg gggatctgtt cggtatgacc
    15721 atgaatgccg gaatgggttg gtctccggac gagctggatc gggacggtat tttactgctc
    15781 agtacactcg gtggcttacg cggcgcagac ggtaaacccg tggcgctggc gttgcaccag
    15841 gggcattacg aactggacat ccagatgaaa gcggcggccg aggttattaa agccaaccat
    15901 gccctgccct atgccgtgta cgtctccgat ccttgtgacg ggcgtactca gggtacaacg
    15961 gggatgtttg attcgctacc ataccgaaat gacgcatcga tggtaatgcg ccgccttatt
    16021 cgctctctgc ccgacgcgaa agcagttatt ggtgtggcga gttgcgataa ggggcttccg
    16081 gccaccatga tggcactcgc cgcgcagcac aacatcgcaa ccgtgctggt ccccggcggc
    16141 gcgacgctgc ccgcaaagga tggagaagac aacggcaagg tgcaaaccat tggcgcacgc
    16201 ttcgccaatg gcgaattatc tctacaggac gcacgccgtg cgggctgtaa agcctgtgcc
    16261 tcttccggcg gcggctgtca atttttgggc actgccggga catctcaggt ggtggccgaa
    16321 ggattgggac tggcaatccc acattcagcc ctggcccctt ccggtgagcc tgtgtggcgg
    16381 gagatcgcca gagcttccgc gcgagctgcg ctgaacctga gtcaaaaagg catcaccacc
    16441 cgggaaattc tcaccgataa agcgatagag aatgcgatga cggtccatgc cgcgttcggt
    16501 ggttcaacaa acctgctgtt acacatcccg gcaattgctc accaggcagg ttgccatatc
    16561 ccgaccgttg atgactggat ccgcatcaac aagcgcgtgc cccgactggt gagcgtactg
    16621 cctaatggcc cggtttatca tccaacggtc aatgccttta tggcaggtgg tgtgccggaa
    16681 gtcatgttgc atctgcgcag cctcggattg ttgcatgaag acgttatgac ggttaccggc
    16741 agcacgctga aagaaaacct cgactggtgg gagcactccg aacggcgtca gcggttcaag
    16801 caactcctgc tcgatcagga acaaatcaac gctgacgaag tgatcatgtc tccgcagcaa
    16861 gcaaaagcgc gcggattaac ctcaactatc accttcccgg tgggcaatat tgcgccagaa
    16921 ggttcggtga tcaaatccac cgccattgac ccctcgatga ttgatgagca aggtatctat
    16981 taccataaag gtgtggcgaa ggtttatctg tccgagaaaa gtgcgattta cgatatcaaa
    17041 catgacaaga tcaaggcggg cgatattctg gtcattattg gcgttggacc ttcaggtaca
    17101 gggatggaag aaacctacca ggttaccagt gccctgaagc atctgtcata cggtaagcat
    17161 gtttcgttaa tcaccgatgc acgtttctcg ggcgtttcta ctggcgcgtg catcggccat
    17221 gtggggccag aagcgctggc cggaggcccc atcggtaaat tacgcaccgg ggatttaatt
    17281 gaaattaaaa ttgattgtcg cgagcttcac ggcgaagtca atttcctcgg aacccgtagc
    17341 gatgaacaat taccttcata ggaggaggca actgcaatat taaatgccag acccagccat
    17401 caggatttac ttcccgatcc tgaattgcca gatgataccc ggctatgggc aatgcttcag
    17461 gccgtgagtg gtgggacatg gaccggttgt atttatgatg taaacaaaat tggcgcggct
    17521 ttgcgcgatt ttatgaataa aaactgaaag cataaataaa aacatccaga cacctgaatt
    17581 atccgactct gcatcattcg ttatttgctc agtcggataa tgatgggtcg ctaccctgtt
    17641 atgagaggac gttatgccac taattatcgt tgtggcaggg attgctttac tcctgctttt
    17701 aaccataaaa attaagctta atacgtttgt ttcgttaatt attgtctcga ttgctgtcgc
    17761 catcgccagt ggtatggatt tgagtaaagt cgtcacctca gtcgaatccg gacttggcgg
    17821 tacgctgggc catattggtt tgatatttgg cttcggtgtt atgctcggtc gcttgctggc
    17881 cgatgcgggt ggtgcgcagc gaatagcgct gaccatgctg aattatttcg ggaaaaataa
    17941 acttgactgg gcagtggtct gttcggcgtt tattgtcggt atcgcacttt ttttcgaagt
    18001 cggtttaatt cttctggtac ctattttatt cgctattgcc cgtgaagcaa aaatatcacc
    18061 gatgtttatg tgtgtgccga tgctttctgg tttgctggtc gcacacggtt ttttgccgcc
    18121 gcaccccggc ccgactgtta tcgccaggga atatggcgct gatgtaggat tggtactgat
    18181 atacggtatc attgtcggca ttccaacctt tattctttgc ggcccggtat tgaataaatt
    18241 ctgccagcgc attattccgg acgcctttaa aaaagagggg aatatagcct ctctcggcgc
    18301 gacccgccga ttcagcgaaa gcgaaatgcc aggctttggg atcagttttc tcacggcaat
    18361 gctgccggtg atcctgatgg cggttgtcac catcatccag atgactcatg ccaaaagtgc
    18421 cgcggattca ggcttatttt acaacgtgat actgttctta ggtaattcaa cgatcgccat
    18481 gctgatttca ctgctattcg cgatctatac catgggcctg gggcgcggga aaacaatccc
    18541 tgacctgatg gattcttgcg gaaaagctat cgcgggtatt gccggtctgc tgctgattat
    18601 cggtggtggt ggtgcattca agcaagttct catcgactct ggcgttggtc agtatatttc
    18661 caccctggtt tcgggtatgg atattaaccc gatcctgatg gcctggggcg tggccgcatt
    18721 cctgcgtatt tgtctgggtt cagccaccgt cgcggcgatc tctaccgccg gtctggtcat
    18781 cccattgttg gcagtacatc ccaataccaa cctggccttg attacgctgg ctaccggtgc
    18841 cggatcctgt atttgttctc acgttaacga tgccagtttc tggatgatta aagacttttt
    18901 tggcctgacc accaaagaaa cgctgttatc ctggacgctg atgtccacac tcttatccat
    18961 cagtgggctg atttttattc tgctcgccag cttagtttta taatcgggtt cttgccagtg
    19021 agccaacctg ttgttcactg tcccccataa tttagtgagt gacggcaacc gtctgcacgg
    19081 tgacgaatcg ctggcgatca aaaaggggcg atattgaacc ttcgattatg cttgatgggt
    19141 gtattctcag taatactgta aagccggagc atgtctccgg ctttacagta tgggggaaca
    19201 gcgggcgatc gctcaaatgg tatgctggtt tatttcatgt aaaagaataa aatatctcta
    19261 taaaatcatg ccactggcaa tctgcatcaa ctctccgggt aaacagggtt aagatgagca
    19321 tttccccggt ttaatacagg tatacaaaag tggatagaga tcaaacaaac agcagcctgt
    19381 tcaatgatga tcccgtactt catgcaacat ggctgtacta tcaggaaggt aaaagccaga
    19441 ctgaagttgc agctatcatg ggggtgtccc gcgtcacggt tgtcaaatac ctgcaaacag
    19501 cccgggaaaa tggactggtt catatcaacc tggatgtgaa tgtctttggc tctatcgacg
    19561 ctgctttgca aattcgcgat aaattcaatc tccagagagt gatcatcgtg ccggatggcg
    19621 aacatgccgg aaaacgtgat gatacaaagc tgatgcggac tcgcctttca cgtgccggtg
    19681 gcatgtatct gaatcaggtg attgagaatg gtgatgtgct gggggttgcc tggggaagaa
    19741 caatccatca gatgagcaaa accatgacgc cgaagtcatg taaaaatgtc accgttatac
    19801 agatgctcgg ttccatgcca tctcagccag acctgacgat cattgaatcc tcatcgcaaa
    19861 tcgcctacaa gctttcaggc cgtgtcgcct cgctgcacgt gcccgccgtg gtatcaagcg
    19921 ccagactggc catggagtta caggcagagc ctattattcg atcgaatttt gacgttctga
    19981 ctcgctgtac gaaagccttt tttgtcgtgg gtaacgcgct tgatgaaaat cccctgattc
    20041 gggtgggcgt gctgaataag aaggaaatgc agacgtatcg ggatttaggc gcagtaggtg
    20101 tcatttgtgg tcgcttctat gacaaggaag ggatgcccgt cgtggctgat gttgaccagc
    20161 gtattctggg aatcagtctg gctcagctaa ggcagattga acggaagatc ttccttgctg
    20221 gtggggaaag aggctatgac gcaacgttgg gtgccctgct gggaggatat gtcacggacc
    20281 tgattgttga tgaaggaacg gcagagtttt tgctggcgtg tgaattacca cactaaaaga
    20341 atgaacaact gccccatgta gatatggggc aggattttat tgattagcgg cgataataag
    20401 aacaataccc acaatgtatg aaataaacat ggtcagcagc agtttattgg ttccggctgg
    20461 cgcactggca aattctctcc agatgaagac tccccagatc acggcaatca ttgtggcccc
    20521 ctgtcccaga ccgtaggaaa tggcataccc cgcctgacca gacgcaatca gactgaatgc
    20581 cagaccgaca caccaaatca taccgcccag ccagccgcaa acatgatccc gtaaactccc
    20641 ggaaaaatac attttcccgt tgaccggttc tcctgaaata ggttttttca tgaccagcct
    20701 gttaaggaca aagttcgaca aaaacagccc cacggcaaac agaacaagtg ctgagtaagg
    20761 cgtcatcaga ccgctggcag gctggctgaa attgtcagac atggagtccg ccaggaagcg
    20821 gaaaaaccat cccatgatga tacccgccag aatggcagta atgagacctt tattacggcg
    20881 agatttatcc gcctcctgcg tgacacgacc ataggctatc gcggtaaaaa taatggcgat
    20941 cacaacgcag acgacaccga ggaacagaat gagcggatcg ccctgcggat tgccgatata
    21001 ggtggtgatc acacccagag ccagtgccag ccccacaccc acaggaaaag ctaccgccat
    21061 accggcaaga ttaatagaag cgaccagaag aatattggaa atattgaaga taatccctgc
    21121 cagtattgcc gacatcagac tactgctgct cgcctgctga atatcaggta taaaactgcg
    21181 accggcttcg cccatagaac ccagcgtgaa ggcaaataac aaagaacaca gcaataatcc
    21241 aattgaataa tcccaataga atagagggaa ctcccatttt ttattgctga caagtttagt
    21301 tgtattcgcc catgaacccc agcaaatcat ggtaataaaa cacattatta tggcgacagc
    21361 atacgattcc acgataaaca taaataactc cttgtggtag ttataatcaa tcgttgtgga
    21421 cagagggaat agcagccatt ctgcgaatgg ctgtcatatg ttattaatta aaataaagct
    21481 gtaattacgc tttcaaacca tacgttttga atttttcaag gacgatggtc atttcggctt
    21541 cagacagcac gggaacatcg tgatgtaatc cgccacattt gatgtataaa tcagccagcg
    21601 tctcggtttc acccgccagc cacaacgtct tttccagcgt gacgtccatg gccagcatac
    21661 cgtggtgctg catcagcaag gacttacttt ctctgattcc tttagataca ccgtcggcca
    21721 gttcaggact gccaaaggta taatagggaa tacagggaat atgatccgtc cccgataccg
    21781 ccaccatata atgaatggcc ggaattgaat gattatgtat tgccaccgcc gtggaattca
    21841 ccgcatgcgt atgtatgaca gcattcaatt ctggtctggc tttataacac gccagatgaa
    21901 ttaaccattc acttgatggt attttacccg cttcaggttt tcccttatcg tctacaaaga
    21961 caatcatgtc cggcgtcatc tttgaataag cgatcccact cggcgtaatc aacatgccac
    22021 cggtatacct ggcactcaca ttacctgatg ttccttgatt aagacctgac gcattcattt
    22081 cccgacaagt ggaaatgatt ttttctgcta acttaatccg ttccattcct gatccttaat
    22141 agcaatggcc gctgttttaa tgctgttccc cctggagtgc tttactcttc aggtcagcaa
    22201 acatatccgc cagcgctgac ttactggcag cacagactcc ccgctcagta atgaagccgg
    22261 taatgtatcg ggcaggcgtg acgtcgaaag cgtagttccc acaacgggtt ccttcagggg
    22321 cggtattgac ccagctcaat tctccctgag ggtttatccc atagacatgg gactgctctt
    22381 ttccgtcacg ttgctcaatc ggaatgcttt ttccgtcctc gatagtccag tcaatagtgg
    22441 gagaaggtaa cgcgacgtag aagggaacat ggttatcatg ggccgccagc gccttaagat
    22501 aggtaccaat tttattacag acatcccctc tggccgtggt tcgatcggtc ccaacaatgc
    22561 agagatcaac gtcaccgtgt tgcatcaggt ggccacccgc gttgtcggcg atcagggtgt
    22621 gaggaatacc gtgtgagccc agttcaaatg ccgtgagtcc gccctgatta cgtggccgcg
    22681 tttcatccac ccagacgtga acagggattc cgttttcatg cgctttgtag atgggagaaa
    22741 gcgcggttcc ccagtctaca gtcgccagcc agcctgcgtt acaatgtgtc aggatattga
    22801 ccacactccc ggccggtttt ttctgggcta tttcgcggat gacttctgca ccatgtaatc
    22861 caatttgttc acacaacgcg acatcttcct cggcaatttc tgccgccagc gcccaggcca
    22921 catccttgcg ctctgataca cagagatcct tcagtgtgtc tatcatcctg tcgagggccc
    22981 atttgagatt aattgccgtt ggtcgggttt tgataagaag gtcatagtaa cgctgcagac
    23041 ccatgtcact ggcatcgtga ttcattccca gcgcgatgcc ataggctgca acgacaccaa
    23101 taagcggagc gccacgaacc cacatatcct gaatagccgt agcggccatt tccgcagagg
    23161 taagcgccac cacctcgaac ttaaaaggca acttagtctg atcgatgatt tctaccgctt
    23221 ttccatcccc ggaaacccag accgtacgat agtgtttacc tttaatattc ataatgacct
    23281 cttacttaac cttcgcagac tggatgacgg aatccatatt tttaaactgg cgggcattga
    23341 cgataagatc gcgcgccatg gtcaatgcac gtcgttcaca tcctgcgcga agctctgtat
    23401 tttcaatctg tttcagctcg gcaacgccag caaaaccaat gatccggcga ttcatttcca
    23461 tgccggcatt caccagggaa tcttcaagca gcgtggcaaa aaactgatcc tgtgcgtcct
    23521 caagagcggc cctgttctgt tgatacattt ctgtcgacca cgcgtcgccc tgtgttttgt
    23581 tgtcccagag ctggcggaac tcccgggtga aaacggacca ggtttggaca atctgctgaa
    23641 gcagccattc ctgatagtca gcgcgacgtt gctcattcgc atcccaccca gggcgtgaga
    23701 agtacgccag caggaggttg ccaatatagt tgccgatatc aaacgccatt ggccccatga
    23761 aaccgaactc cggatcaatc actttggtat ccgtgtcggt caccatgatt gagcctgaat
    23821 gaaggtcgcc atgtaacaat gcctgcgctt ctgtcataaa tttgtactta taacgcatgg
    23881 caacctggat catctctaca tcagcccagg ccttatggac ggcatcgtcc agctcaggag
    23941 aagtccagtt attccgttca gcgttgtaat agggctctgt gaagatcaga tcttccgtaa
    24001 ttttgcacag ctcatgattc aacgcgaact cggcagtaag cgcttttttc tgttctgctg
    24061 acatgccaat gtcagacgta ttgaagagag tctgtgctaa aaatctgccg atatcttcag
    24121 ctagtttagg gaatttttta ccgttaatca gttccttacg cagaatcacg tgaggagtca
    24181 gatactccat ggcaaacagc gccatttccg ggtcgtaaaa atagacctgc ggaatcagtg
    24241 tgtgacccgc gtacttggct tcctgacata agacgttgta ctcaaagccc gcacggctca
    24301 gagataaagg ccatccttcg cccccggcac gaagccaggg cagagcttgt tttacaatga
    24361 tggttttatg tgtcccctca acgataaaca ccatgttcag gtttccgtcc cccacttctg
    24421 aaactttcca ctcttcaggt aaccctcccg gttcgcatga aggggtcagt ctggacgaga
    24481 gataacccgg cagcgtgtca catgtcagcg gcttgtaacc tgatggaatt gaatccgtca
    24541 taacagttct ccttgtctac acagaatgat aatcaacttg tcatgtgaca actatacatt
    24601 atcaaatgta aaattatttg tgacacatgt agattttttg aactgtataa aattaaaaat
    24661 g
//
LOCUS       DAZFRG010000048        24518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFRG010000048 DAZFRG010000000
VERSION     DAZFRG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.048"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..1385
                     /gene="gltS"
                     /locus_tag="ACHKUC_001535"
     CDS             180..1385
                     /gene="gltS"
                     /locus_tag="ACHKUC_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO6617812.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(1419..3500)
                     /gene="recG"
                     /locus_tag="ACHKUC_001536"
     CDS             complement(1419..3500)
                     /gene="recG"
                     /locus_tag="ACHKUC_001536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO6617813.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSTNDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(3506..4195)
                     /gene="trmH"
                     /locus_tag="ACHKUC_001537"
     CDS             complement(3506..4195)
                     /gene="trmH"
                     /locus_tag="ACHKUC_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO6617814.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTISDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(4202..6310)
                     /gene="spoT"
                     /locus_tag="ACHKUC_001538"
     CDS             complement(4202..6310)
                     /gene="spoT"
                     /locus_tag="ACHKUC_001538"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO6617815.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(6329..6604)
                     /gene="rpoZ"
                     /locus_tag="ACHKUC_001539"
     CDS             complement(6329..6604)
                     /gene="rpoZ"
                     /locus_tag="ACHKUC_001539"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO6617816.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(6659..7282)
                     /gene="gmk"
                     /locus_tag="ACHKUC_001540"
     CDS             complement(6659..7282)
                     /gene="gmk"
                     /locus_tag="ACHKUC_001540"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO6617817.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            7540..9222
                     /gene="ligB"
                     /locus_tag="ACHKUC_001541"
                     /pseudo
     CDS             7540..9222
                     /gene="ligB"
                     /locus_tag="ACHKUC_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            complement(9219..9836)
                     /gene="yicG"
                     /locus_tag="ACHKUC_001542"
     CDS             complement(9219..9836)
                     /gene="yicG"
                     /locus_tag="ACHKUC_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO6617818.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10128..10952)
                     /gene="dinD"
                     /locus_tag="ACHKUC_001543"
     CDS             complement(10128..10952)
                     /gene="dinD"
                     /locus_tag="ACHKUC_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO6617819.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(11174..12037)
                     /gene="yicC"
                     /locus_tag="ACHKUC_001544"
     CDS             complement(11174..12037)
                     /gene="yicC"
                     /locus_tag="ACHKUC_001544"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO6617820.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12164..12880
                     /gene="rph"
                     /locus_tag="ACHKUC_001545"
     CDS             12164..12880
                     /gene="rph"
                     /locus_tag="ACHKUC_001545"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO6617821.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12946..13587
                     /gene="pyrE"
                     /locus_tag="ACHKUC_001546"
     CDS             12946..13587
                     /gene="pyrE"
                     /locus_tag="ACHKUC_001546"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO6617822.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(13624..14220)
                     /gene="slmA"
                     /locus_tag="ACHKUC_001547"
     CDS             complement(13624..14220)
                     /gene="slmA"
                     /locus_tag="ACHKUC_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO6617823.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(14327..14785)
                     /gene="dut"
                     /locus_tag="ACHKUC_001548"
     CDS             complement(14327..14785)
                     /gene="dut"
                     /locus_tag="ACHKUC_001548"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO6617824.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(14763..15983)
                     /gene="coaBC"
                     /locus_tag="ACHKUC_001549"
     CDS             complement(14763..15983)
                     /gene="coaBC"
                     /locus_tag="ACHKUC_001549"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO6617825.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKALLGQLL
                     LDEIVTRYDEKNRR"
     gene            16155..16823
                     /gene="radC"
                     /locus_tag="ACHKUC_001550"
     CDS             16155..16823
                     /gene="radC"
                     /locus_tag="ACHKUC_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6617826.1"
                     /translation="MKNNAQLLMPREKMLKFGISSLTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17040..17276
                     /gene="rpmB"
                     /locus_tag="ACHKUC_001551"
     CDS             17040..17276
                     /gene="rpmB"
                     /locus_tag="ACHKUC_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO6617827.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            17297..17464
                     /gene="rpmG"
                     /locus_tag="ACHKUC_001552"
     CDS             17297..17464
                     /gene="rpmG"
                     /locus_tag="ACHKUC_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO6617828.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            17562..18371
                     /gene="mutM"
                     /locus_tag="ACHKUC_001553"
     CDS             17562..18371
                     /gene="mutM"
                     /locus_tag="ACHKUC_001553"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO6617829.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18410..18889)
                     /gene="coaD"
                     /locus_tag="ACHKUC_001554"
     CDS             complement(18410..18889)
                     /gene="coaD"
                     /locus_tag="ACHKUC_001554"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO6617830.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(18897..20174)
                     /gene="waaA"
                     /locus_tag="ACHKUC_001555"
     CDS             complement(18897..20174)
                     /gene="waaA"
                     /locus_tag="ACHKUC_001555"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO6617831.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            20587..21645
                     /gene="rfaQ"
                     /locus_tag="ACHKUC_001556"
     CDS             20587..21645
                     /gene="rfaQ"
                     /locus_tag="ACHKUC_001556"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO6617832.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            21642..22766
                     /gene="waaG"
                     /locus_tag="ACHKUC_001557"
     CDS             21642..22766
                     /gene="waaG"
                     /locus_tag="ACHKUC_001557"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6617833.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            22759..23556
                     /gene="rfaP"
                     /locus_tag="ACHKUC_001558"
     CDS             22759..23556
                     /gene="rfaP"
                     /locus_tag="ACHKUC_001558"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO6617834.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEVLKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFSAPLKDILKQEQGLLSQAEEKATKIRERTIRKSL"
     gene            23572..>24518
                     /gene="waaO"
                     /locus_tag="ACHKUC_001559"
                     /gene_synonym="rfaI"
     CDS             23572..>24518
                     /gene="waaO"
                     /locus_tag="ACHKUC_001559"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO6617835.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDQNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPDADIQRLSQLAKSYHTCIQIHLVNCERLKVLP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGTLKPLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFICPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPMNSNYARYCAKHN"
BASE COUNT         5820 a         6056 c         6495 g         6147 t
ORIGIN      
        1 gtactttttt atataacttt tagttgtttt ctgcttaaaa tccatgccat ttttctagtt
       61 tcagctaaga attcgaacca ggaaagcatg actcgatact cgtcatattt cgccttactg
      121 caaatgcgcg atgaagtatg acgagtatga aagagtgatg cggacacaaa ggagtaacta
      181 tgtttcatct cgatacttta gcaacgcttg ttgccgcaac gctgacgttg ctgctcgggc
      241 gcaagttggt ccattccgtc tcctttttga agaaatacac cataccggaa cctgttgcgg
      301 gtggtttatt agtggcgctg gcgctgctga tactgaaaaa aagcatgggc tgggaagtca
      361 actttgatat gtccctgcgc gatccgttga tgctggcttt cttcgccacc attggcctga
      421 acgccaacct tgccagtctg cgagctggtg ggcgtgtggt gggggttttc ctgattgtgg
      481 ttgttggcct gttggtgatg caaaatgcca ttggcattgg tatggctagc ctgttagggc
      541 ttgatccgct gatggggctg ctggccggtt ctattacgct ttccggcggt cacggtacgg
      601 gtgctgcatg gagtaaattg ttcattgaac gttatggctt caccaatgcg acagaagtgg
      661 cgatggcctg tgcaacgttc ggtttggtgc tgggcggctt gattggcggt ccggtggcac
      721 gctatctggt gaaacactcc accacaccga acggtattcc ggatgaccag gaagttccga
      781 ctgcgttcga aaagccggat gtgggccgca tgatcacctc gctggtgctg attgaaacta
      841 tcgcgctgat tgctatctgc ctgacggtgg ggaaaattgt tgcgcaactt ttggctggta
      901 ctgcttttga actaccgacc ttcgtctgtg tactatttgt tggcgtgatt ctgagcaacg
      961 gtctgtcaat gatgggcttt taccgcgtct ttgagcgagc ggtatccgtg ctgggtaacg
     1021 taagcctgtc gttgttcctg gcgatggcgc tgatggggct gaaactgtgg gagctggctt
     1081 cgctggcgtt gccgatgctg gcgattctgg tggtacagac catcttcatg gcgttgtatg
     1141 ccatcttcgt tacctggcgc atgatgggca aaaactacga tgcggcagtg ctggctgcgg
     1201 gtcactgtgg ttttggcctc ggtgcaacgc caacagcaat cgccaacatg caggcgatca
     1261 ctgaacgctt tggcccgtcg cacatggcgt tcctggtcgt gccgatggtc ggtgcgttct
     1321 ttatcgatat cgtcaatgca ctggtgatta agctgtattt gatgttgccg atttttgccg
     1381 gttaaccgat gaagcggcgg tcttctcacc gccgcttttt acgcattcga gtaacgttct
     1441 gtttctggca tccagcgttc tatcagggct tttgcctgct gtgggtaacg ttcgtgaata
     1501 tgtcgcgcca ggcgctgaac ttccgggatc atcgcctgat cgcgcagtaa atccgccact
     1561 ttaaattcag cattccccgt ctgacgtgta cctaacaatt cgccagggcc gcgaatctcc
     1621 agatctttct gtgcaatcac aaaaccatcg ttgctgtcgc gtagtacctg caggcggatt
     1681 tgcgcggttt tggagagcgg cgttttgtag agcagcacgc agtgagaagc caccgcgcca
     1741 cgacctacac gcccgcgaag ctggtgtaac tgcgccagac ccagacgctc cgggttttcg
     1801 ataatcatca gactggcatt gggtacatca acaccgactt caatgacagt agtggcaacc
     1861 agcaggtgta gctcgccttg tttaaacgat gtcatcacag cctgtttctc ggcgggtttc
     1921 atccgtccgt gtaccaggcc aacattcaac tctggtagcg ccagtttcaa ctcttcccag
     1981 gtcgcttccg ccgcctgcgc ttccagtaat tccgactctt caatcaacgt acaaacccag
     2041 tacgcctgac gcccttcagt catacaggcg tggcgcacgc ggtcaatgat atcggtacgg
     2101 cgggtatcag gaatagcgac cgtagtcact ggcgtgcggc ctggcggcag ctcatctatc
     2161 accgaggtat cgagatcggc atacgcagtc atcgccagcg tgcgggggat gggcgtggcg
     2221 gtcatgatca actgatgcgg gtggaagccc tgctgctggc ctttctccca taacgccaga
     2281 cgctgatgca cgccaaaacg atgctgttcg tcgataatca ccagcgccag gccgttaaac
     2341 tgcacctgtt cctggaaaat ggcgtgcgta ccaacaatca tctgcacctg accgctggcg
     2401 atggcctctt gctgagccag tcgtgcttta cctttctgtt ttccggcgag ccagcctact
     2461 tcgataccga gcggtgcaaa ccagttacgg aagttattgg cgtgctgctc ggcgagtaat
     2521 tcggttggtg ccatcagggc cacctgtttg ccgtgggcaa ccgcacgcaa cgcggcgagg
     2581 gcggcgacca gcgttttacc ggaacctaca tcgccctgca ccaggcgcat cattggcaca
     2641 tccagcgcca tatcgcgctc gatctccgcc actacgcgtg cctgtgcgcc cgttggcttg
     2701 aacggtaagg cggcgaggag tttatttttc agcgcgtcat tggtgctcag cggctgagca
     2761 tgaaaacgtt gtgcaccggc acgtaacgcc aacatgctga ggttgtgcgc cagcaactct
     2821 tccagaatca gacggcgttg tgccggatgc tgcccggttt cgagatcgct aagctgcagc
     2881 gtcggcggtg ggcgatgcaa agtgcgcaac gcttccggta gcgtcatcat tccttgtgac
     2941 agttccggcg gcaggagttc ttcaatggcg catgtgtcga gcagatccag cgcctggtcg
     3001 gttaatttac gcagcgtagc ctgctttacg ccttccgtag ttggataaac cggtgtgagc
     3061 gtttcctgta actctggcgt gctgagatcg ccctgcacac ggtattccgg gtggatcatc
     3121 tccgcaccat atttgccgcg ctttgcttcg ccataggcca gcacgcggcg gcctgtcgcc
     3181 aggctatttt tcattgccgc attgaagttg aaaaagcgca tagtgagaat gccggaaccg
     3241 tcgctgatct ggcaggtcat catccgccga ccgccgaagg agatattgca gttcagcact
     3301 tcgccttcca ccgtggcata aacacccggc agtagttcac cgatggggta gagatgtgtg
     3361 cgatcttcgt agcgcagggg aaggtgtaag agtagatcct gcacggtatg caggttgatt
     3421 ttcgccagct tgttactaag cgctgcgcca acgcccgtta gggaactgag cgggacagca
     3481 tctaacaggc gacctttcat cgcacttacc ctgcagcctg catggtggac caccagtcag
     3541 catcggcttc aatctcgcct tgctgattaa cgtgggggta gggcagacct ttgcgttttg
     3601 cgactttcgc cagcaccgga tagccgcctt caaacagcag gcgttgttgc tcggcttccg
     3661 gcaacatgct gttttcacgc aggtacatgc ccgcattttg ccgctggcgc tgggcttcgt
     3721 aaagaatgag ggctgaggca acggaaacat tcagcgactg caccatgccg atcatcggaa
     3781 tgatgatatc ctgatccgcc agggccaacg cttcctgcgt gatgcccgtt ttctcctgtc
     3841 ccatcaaaat acaggtcggg cgagtgtaat caatttcgcg gaaatcaaca gcgttatcag
     3901 aaagatgggt tgccagaatc tgcatgccct ggcctttcag atgagcgacg gcatcactaa
     3961 tggtgcggtg cgttttcacc tgtacccagc tgttactacc cgcagctgcc gaagccatgg
     4021 tgcgcatccg gctgccaggc cagacagcat gaacttcatg tacgccaacg gcatctgcgg
     4081 tacgaataat cgcagaaacg ttatgaggtt tgtggacctg ctccatgcag acggtcagat
     4141 caggctgccg cctggcgagc atttcgcaga tgcgtgcata acgtgttggg ttcataaaac
     4201 attaatttcg gtttcgggtg actttaatca cgtctggcat cacgcggatt ttgcgcatga
     4261 tattcgccag atgcacacgg tcacgggcgg tcagacgaat aaaggcgcta tagacgcgac
     4321 catctttctc ttccgtattc aaactttgaa tattcgaggt cgtggtgtta attgccgccg
     4381 tcaggtttgc cagcgcgccc tgatgattga acatctccac cttgatttcg gtgatgaatt
     4441 cctgcgccgt ctctttatcc cattcaacag ccataaactt ctctggctct ttctggtagc
     4501 cacggatgtt acggcaggat tcatggtgga tcaccagacc tttaccgggg ctgacgtggg
     4561 cgataatcgg gtcgccagga attgggcggc agcatttcgc aaaggtgatc agcacgccat
     4621 cggcaccttt aatgggcaga tgtccgtgac tttgggttgc cggtggaatg gaggcgtccc
     4681 catgttgcag atttttcgcg accaccacgc tcattgcgtt accaaggccg atttctgcca
     4741 gcagatcgtc aagcgttgcc agcttcatgc ggtccagctc gcgctgaata ttttcctgcg
     4801 ggatttcatt gagcttacgg ctaccaccca acgcatggtt gagcagacga cggcccaggc
     4861 ttacagaatc atcacgcttg aggtttttca gcaactgacg aattttggcg cgcgctttcg
     4921 agctgacgac aaagttcagc cacgcggcat tcgggcgagc acccggtgcg gtaatgattt
     4981 caacggtttg accgctagta agcggctgcg acagcgggta aggctggcgg tcgacgcgtg
     5041 cgcccacgca ggcatggccg atatcggtat gcaccgcata agcgaagtcg acgggcgttg
     5101 caccggcagg cagttcgaca atgcgccctt ctggtgtgaa aacgtaaatc tcatccggga
     5161 agagatcgga tttaacgctc tcgataaatt caaacgagct accggcactc tgttgcagct
     5221 ccagcaggct ttgcatccag cgctgggcgc gtatttgcgc ggtagtgctg gtttcgccgt
     5281 gctctttata agcccagtgc gcggcaacac ccatctccgc catctgatcc atatcttcgg
     5341 tacggatctg gacctcaacc ggcacgccgt gcgggccaat catcgaggtg tgcaaagact
     5401 gatagccgtt cgcttttgga atggcgatat agtctttcac gcggcccgga cgcggcttgt
     5461 acaggctgtg catctggccc agcacgcgat aacaggtgtc agaatcattg acgatcacgc
     5521 ggaaagcgta gatgtccatg attgagtgaa aacgctgctc tttgagcacc attttgcagt
     5581 aaatcgaata aagatgcttt tcgcgaccac tgacgcggca cggtattccc gcttcctgca
     5641 aacgcccttc gatttcagaa agaatcttct ggatcatctc tttacggttg ccgcgcgcgg
     5701 ctttcaccac ttctttgatt acgcgataac ggttgggata cagcgcctca aaacccagct
     5761 cctcgagttc ggttttaatg tggtggatac ctaaacggtg cgccagcggg ctgtatattt
     5821 cgagagtttc acgggcgatg cggcgacgtt tgtccgggcg aagtgagccc agcgtgcgca
     5881 tgttgtgggt acggtcggca agtttgatga gaatgacgcg gatatcctgc accatcgcca
     5941 taatcatctt gcgaaagttt tcggcctgcg cctctttctt atcgcggaac ttgagtttat
     6001 caagtttcga caccccctct accagctcgg cgacgctttt accaaaaagc tgttccatgt
     6061 cctggtaggt ggcgggtgta tcttcaatca catcatgcag cagcgccgcc atcagcgttt
     6121 catagtcgag tttcatctcg gccagaatgc aggcaaccgc caccgggtgc gtgatatagg
     6181 gttcaccgct tgaacgtgtt tgcccctcgt gagcatcacg tgcaacgaga tacgcctgcc
     6241 gcagacgctt gatttggtct tccggcaggt agttttgaat cagctgattc aggctttcaa
     6301 acagatacaa gggcgacccg ctttgtgatt aacgacgacc ttcagcaata gcggtaacgg
     6361 cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg tcgaggatct
     6421 ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt ttatcgtttt
     6481 cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga cgcgcggcga
     6541 ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca gttacgcgtg
     6601 ccatacttaa aaagctccac aggtgaagaa atgactgggc atgatactga aatcaggttc
     6661 agtctgccaa caatttgctg attaaagcgt catgacgctg cttttggcgg ctcatgcgca
     6721 gacgttcggc gcgaataatg gtcttcaaat cggtcaacgc ggtatcgaag tcatcattca
     6781 caatcaggta atcatattcg gcgtaatggc tcatttctgc aacagcttgc gccatacgct
     6841 ttgcaatgac ctcttcgctg tcctgaccgc gaccgcgtag acggcggtcc agttcgattt
     6901 tagacggcgg taaaataaag atactccgcg cgtgcggcat cttctggcga atttgctgcg
     6961 caccctgcca gtcgatatcg agaaaaacat cgacaccggt cgccagtact tgctcaatgg
     7021 cctcacgcga agtgccatag taattaccaa aaacttctgc gtgttcgagg aacgcatctc
     7081 tactaatcat ttctttaaat tcatcatgat taacaaagaa ataatgttca ccgtggactt
     7141 caccaggacg cggttggcgt gtggtgtgtg aaacagaaac ctgggtgtca tacaacggtt
     7201 gggtttttaa taaagcctga atcaggctgg atttacccgc gccactgggg gcagaaacaa
     7261 tataaagcgt gccttgagcc atgagtatct ttcgtatgtg attagcgaaa taaagcctac
     7321 atacgagctt attatacacg gcgcggccat gtgacgtagc ccttgctcac actttttgcg
     7381 caaagttgtt gcattttgcg tagctgcttc cgcatttgcc tgtgattcac tgcaataaag
     7441 acgattctca ggaagcgtcc tcgcagactc cgtttttgcc actcggcttt gctttcacca
     7501 cgttgtatat ctgcctgact atctacagcg gaggtgatga tgaaagtatg gatggcgata
     7561 ttaataagta tcttgtgctg gcaatcatcg gcgtgggcgg tctgtccggc ctggtcgcca
     7621 gccagagcac aggaagaaat ttcccgcctg caacagcaaa taaaacagtg ggacgatgac
     7681 tactggaagg aagggaaaag tgaggtggaa gacggtgttt acgatcagtt aagcgcccgt
     7741 ctcacgcagt ggcaacgctg ttttgggaac gagactcgcg atgtgatgat gccgccttta
     7801 aacggtgcgg ttatgcatcc ggttgcccat acgggtgtac gcaaaatggc ggataaaaac
     7861 gcattaagtc tgtggatgcg cgaacgtagc gatctttggg tgcagccaaa agttgatggc
     7921 gttgcggtaa ccctggttta tcgggacggg aaactgaaca aagcaatcag tcgcggtaat
     7981 ggcctgaaag gcgaggactg gacgcagaaa gttcgcttaa tttccgctgt gccgcaaacc
     8041 gttagcgggc ctttagccaa cagcacgctt cagggggaaa tatttctcaa gcgtaagggg
     8101 catatccaac aataaatggg gggaataaat gcccgcgcaa aagttgctgg cctgatgatg
     8161 cgccagggga atagcgatac gctaaactca ctggctgttt ttgtctgggc atggccggat
     8221 ggaccgcatt taatgaccga tcgtttaaag gatctggcta ccgcaggttt tactttaacg
     8281 cagacgtata cccgcgcggt taaaaatgct gatgaggttg cgcacgtacg caatgagtgg
     8341 tggaaagcaa aattaccctt cgtcaccgat ggcgtggttg tgcgagcggc gaaagaaccc
     8401 gaatcccgtc attggttacc gggccaggcg gagtggctgg tggcctggaa atatcaacct
     8461 gtagctcagg ttgccgaagt gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata
     8521 tcggtggtgg cgtcactcgc acctgtcatg ctggatgata aaaaagtcca gcgggtgaat
     8581 attggttccg tcaggcgctg gcaggagtgg gatattgcgc ctggtgatca gatcctcgtc
     8641 agccttgccg gtcaggggat tcctcgcatt gatgatgtgg tctggcgcgg agcagaacgt
     8701 acaaaaccga caccaccaga aaaccgcttt aactcgttga cctgctactt tgcttctgat
     8761 gtttgtcagg aacagttcat ttcacgctta gtctggctgg ggtcaaaaca ggttctgggg
     8821 cttgatggca ttggtgaggc cggctggcgc gccctgcatc agactcatcg ctttgagcat
     8881 atcttttcct ggcttttatt aacgccagag caattacaga acacgccggg gatcgcgaaa
     8941 agtaaaagtg cgcagttatg gcatcagttt aatctggctc gtcagcagcc ttttactcgc
     9001 tgggtgatgg caatgggaat accgctaacc cgggcggcgc ttaatgccag tgatgaacgg
     9061 tcctggtcgc aacttttatt tagcacggaa cagttctggc agcagcagcc ggggactgga
     9121 tcggggcgcg ccagacaggt tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc
     9181 agttggctgg ctgcccagca gatcacaggt tttgaacctt agtgaccttc gtggctgtag
     9241 taaaagaccg gtaatcccag cttaagacgt agcgccagta aacgggcaaa aaagccgaat
     9301 accagggtgg agataatcac aacatcatgg ttagaaacat agtgttgcag cgcgatgtac
     9361 agcacggcgg aggcaaatga gacaccggca tacaactctt tctggaatac cagtggaatg
     9421 cgtttacaga acatatcgcg caaaacgccg ccaaacacgc cggtggttac cgccgcgaca
     9481 acggcaataa ttgggccgtg ccccatatcc agtgcaacct gtgcgccgat gatagaaaag
     9541 accaccagtc cgagcgcatc gagcaccaga aacactttgc gcagataagg cattacagga
     9601 gcaacgatag tggtgagtac tgcggcggta gcgacgataa tcacatattc cgggtgtttg
     9661 acccagccga gcggatagtg gcccagcaga atatcgcgca ctgaccctcc gccaattgcc
     9721 gtggcggtcg caataataat tacgccaaat gtatccatgc gccgtcgtcc ggccgccagc
     9781 gcccccgtca tggcttcggc agtaatgcca accaaataca aaatgtgtaa cagcatggtt
     9841 ctctccctaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac gattgagatt
     9901 ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt ttctgtagcg
     9961 tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata ggttaaaatt
    10021 gaggggtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc tggactttgg
    10081 cttacgcatc gggtatctca atcaacaaaa agagttttta ttttctgcta tttcttctct
    10141 gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg caactcctca
    10201 ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgcgacccac gtcaaagtgt
    10261 gtggtattag cttgctgttt tgaattcacg ccatcccgct tgagtttttc ttctgtttgg
    10321 gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag gatcttctga
    10381 ctctttttca gccccttccg ctgatggatg gctttctgat ctaatccacc atacagtccc
    10441 cggtaaccat gattctgaaa tatggcgaaa tcgatagctg tcgctacccc agcttgctgc
    10501 gctgcctcaa ctaattgttt gttatgttct ttcaactcat ttctcagaaa aagacgcttt
    10561 tcgtcttcac gaagttgcct gaaagcttca tcgtcagcca gctcctgccg tcgggtctgg
    10621 atagcaaaat aagtttgccc tgccgcaata accggtttcg cagggtcgcc gttttgtact
    10681 accaggtaac aggcatagcg ggagagatga acatcttcaa gttctcgttg agcccctgaa
    10741 cctaatacaa ccattttggt ggtttcaacg aaatggtcag atgcagcttg attgctggct
    10801 tcgcacgctt gagtcgcgcg ggccagtacc ttttgaaaat tgcgccagtc ccgataatcc
    10861 agtaacggtg ccaattctct cgctgaccaa aattcttgcc cttcagtgcc ataatgccgt
    10921 atttcttcaa aaggttgatg atgttcgttc attgcccact ccatatgata tcgattgcaa
    10981 ggaaagtaag tttacaactg tgtttatata cagttgtgag tatctcacgt tctcactgcg
    11041 ctgggcaaaa aaacatcgca aaatctggag caggcacgca aaacagaatt gaatgttgcg
    11101 gggaggaaaa atgccgctct gtttgagtgg gcggcatttg tgaaggctga aaaggaagaa
    11161 ttggcgttac gagttattca atgttctgaa tctgctcgcg catctgctca atcaacactt
    11221 tcagctcgat ggcggagttt gtcacttcgg cgttgataga tttcgacgca agagtgttcg
    11281 actcgcggtt gaactcctgc atcataaagt ccagacgacg accaacggct tcttttttct
    11341 tcagaatgtt gtaggtctct ttgacatgcg cttcgaggcg gtccagttct tcggcaacgt
    11401 caattcgttg tgccagcaga accaactcct gctccagacg gttgttttcc agttgcacct
    11461 gagcatcttc cagcttcgct accagacgct cacgctgcca ttgcaggatt tccggcatat
    11521 gggcgcggac tttgaccact tcggcggtga cgccttccag acgctgctca atcaacactt
    11581 tcagcgcctg accttcggtt tcgcgcgcga tgataaagtc atccagggtg ccatccaacg
    11641 ccgcgagaat ttcagctgca atcgcgtcga gatcttgctc ctgggctgcc atcacgcccg
    11701 gccagcgtag aatatcaacc gggttgattt ctccttcgtc gctctgcatt tttacccagt
    11761 tcgcggcagt taccagctgt ttagccagtt tttcgttgag gatcagctca ccttgcgcgc
    11821 taacatctgg ctcatagcgc agagtacatt ccactttacc gcgcgtcagg cgagaacgaa
    11881 tacgctcgcg aacgacaggt tcaaggctac ggaactgctc cggcagacga aagtaagttt
    11941 ccagataacg ctggtttacc gagcgcattt cccaggttgc gctcccccat tcacccttga
    12001 tttcacgccg ggcgtaggcg gtcatactgc ggatcataga cgttcctgtt tataaaagga
    12061 gaggtggaag gattatagcc atcgatgcct tgtcaggata ggaataaccg ccggaagtcc
    12121 gtataatgcg cagccacatt tgtttcaagc cggagatttc aatatgcgtc cagcaggccg
    12181 tagcaataat caggtgcgtc ccgttaccct gactcgtaac tatacaaaac atgcagaagg
    12241 ctcggtgctg gtcgaatttg gcgataccaa agtattgtgt accgcctcta ttgaagaagg
    12301 cgtgccgcgc ttcctgaaag gtcagggcca gggctggatc accgcagagt acggtatgct
    12361 gccacgttct acccataccc gtaacgcgcg tgaagcggcg aaaggtaagc agggtggacg
    12421 cacaatggaa atccagcgtc tgatcgcccg tgctcttcgc gcggcagtag atttgaaagc
    12481 actgggtgag ttcaccatta cgctggactg cgacgtgctt caggctgatg gtggcacgcg
    12541 taccgcttcg attacgggtg cctgcgtggc gctggcagat gcgctacaga agctggtgga
    12601 aaacggcaag ctgaaaacta atccaatgaa agggatggta gccgcggtct ctgtcgggat
    12661 tgtgaacggc gaagcggttt gcgatctgga atacgttgaa gactctgccg cagagaccga
    12721 catgaacgta gtgatgaccg aagacgggcg tatcattgaa gtgcagggga cggcagaagg
    12781 cgaaccgttc acccacgaag agttactcac cttgttggcg ctggcccgag ggggaatcga
    12841 atccattgta gcgacgcaga aggcggcgct ggcaaactga tttttaaggc gactgatgag
    12901 tcgccttttt tttgtctgta gaaaagtaag atgaggagcg aaggcatgaa accatatcag
    12961 cgccagttta ttgaatttgc gcttagcaag caggtgttaa agtttggcga gttcacgctg
    13021 aaatccgggc gcaaaagccc ctatttcttc aacgccgggc tgtttaatac cgggcgcgat
    13081 ctggcactgt taggccgttt ttacgctgaa gcattggtgg attccggcat tgagttcgat
    13141 ctgctgtttg gccctgctta caaagggatc ccgattgcca ccactaccgc cgtggcgctg
    13201 gcggagcatc atgaccttga cctgccgtac tgctttaacc gcaaagaggc aaaagaccac
    13261 ggtgaaggcg gtaatctggt tggtagcgcg ttacaaggac gcgtaatgct ggtagatgat
    13321 gtgatcaccg caggaacggc gattcgcgag tcgatggaga ttattcaggc taatggcgcg
    13381 acgcttgcag gcgtgttgat ttcgcttgat cgtcaggaac gcgggcgcgg cgaaatttcg
    13441 gcgattcagg aagttgagcg tgattacaac tgcaaggtga tctctatcat caccctgaaa
    13501 gacctgattg cttacctgga agagaagcca gaaatggcgg aacatctggc ggcggttaag
    13561 gcctatcgcg aagagtttgg cgtttaacga aactcgccgg atgaaaagtc atccggcgtc
    13621 atattactgc aactgtgcgg caattagcgg ccagcgggcg tcaaaatcat ccgtcgggcg
    13681 gtatttaaac tcgctgcgga caaaacgtga cagcatacct tcacagaagg ccaggatctg
    13741 gcttgccagc agggtttcat cggtggtgta accttcaccc tcacgcattc tcttttcacg
    13801 caatacctga cgcaactgcg cttcaatacg ctcgaacagc tggttgatgc gcccttgcag
    13861 acgatcctgt tcgaacatta gcgcatgacc agtgaggatg cgagtcaggc caggattacg
    13921 ctcaccaaaa ccgaggagca acaacacgat cagacgcagg cgcgctgtgg tgtctttctc
    13981 atctttcaga atcaggttga tacgagtaat caggctatct tcgataaact caatcaggct
    14041 atcgaacatg cgggtcttac tggggaagtg gcgatacagt gccgcttcgg aaacgccgac
    14101 agaggcggcc agttttgccg tcgtgatacg ttggcttcca tcgctggatt ccagcatcag
    14161 cgccagagac tgaagtattt cctcgcgacg gttccttttc gcagtttgtt tttctgccat
    14221 gttacaaaat acccctgaaa ataagcactt gccaggcggc acccacgcta tgaccgcaaa
    14281 cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg tatgtgttac tgacgaccag
    14341 agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa atcttccacc agattaaatt
    14401 cagcctgtac taccggaaca aaaatcatct gggcgatgcg ttcgccaggt tgaatggtga
    14461 agctgtcctg accacggttc cacacggaaa tcatcaactg gccctgatag tcagaatcga
    14521 tcaatcctac caggttacca agcacgatac cgtgcttatg tcccaatccg gagcgcggca
    14581 gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc cagcccggtc ggaaccagcg
    14641 tagtgtcacc cggagccagt tctacggcgt cgtcgagaca ggcacgcagg tcaagtccgg
    14701 cagagccaga ggtggcataa gtcgggagcg gaaattcctt cccaacgcgc gggtccagaa
    14761 tcttaacgtc gatttttttc atcataacgg gtcacgatct cgtcgagtaa taattggcca
    14821 aggagcgctt tgcgctcaag cggtaagact ttatctccgt cctgccagaa aaggtgtaat
    14881 gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa catcgttcgc gcagatcaga
    14941 tcaaggtttt tacggatacg tttttgccgg gcgtattctt ccacattatt tgtttcggcg
    15001 gcaaatccaa cgacgtaggg tcgatggtct tttagtgcgg caacgcctgc gacgatatcg
    15061 gggtttttaa ccatttttat tgttaattca tcaccctgcg tggcctgctt tttgattttt
    15121 tctggggcca cggtagctgc gcgataatcc gccacggcgg cgcaaccgat aaaaatattt
    15181 tgctgctgta cagaagcatt cacggcggct tccatttcca gcgcggtcat cacatcaaca
    15241 cggttaacaa acggtggcgt cggtagtgaa accggacctg ataccagcgt gacgttcgcg
    15301 ccacgacggg ctgcggcggc ggcgatagca aaacccatct tgccggagct gtgattagag
    15361 atataacgca cgggatcgag cggttcacgc gtcggaccgg cggtaatcat aatgttcaga
    15421 tgtttcaggt cgttgacggg cgaaaaatgc gctaccgcca tatccacaat ggttaacgga
    15481 tcgagcattc gtcctgggcc gatatcacca caagcctgac tgccgctgtc tggcccccag
    15541 atgagcaaac cacgtgaggc aagcacctct aaattatgct gcgtggcagc ggcacggtac
    15601 atctgctggt tcatggcggg gagcacggct acaggcgcag gtgtagccag acaaatcgtc
    15661 gataccaggt cattcgccat tccggcagca acacgggcaa ttaaatctgc cgtggcaggg
    15721 gcgagaatca ctaaatcagc ccatttaccc agctcaatat ggcccatagc ggcttcggct
    15781 gccgggtcca gcagactgtc ggaaacggga taaccagaaa ccgcctgcaa gctaagtggg
    15841 gtgataaagg cttttgccgc ttcggtcatg gctacgcgga catcggcccc gcgatcgcgc
    15901 aaacgacgca ccagttcagg ggttttatag gcagcaatac cgccgctaac gccgagaacg
    15961 atttttttac cggccaggct catgatgatt tttcctgttg ggtgacacca gaagttggcg
    16021 attttatcac aatacttttg ttgtcgtgcc ttcacctgag attcactttg cgaggcgctt
    16081 tccaggattg aaaactggcc gtcgatttaa aggaacggct atgacaggat gcgagcacca
    16141 caaaggaggt gaaggtgaaa aacaatgcac agctgttgat gccgcgcgaa aaaatgctga
    16201 agtttggtat tagctcctta acggatgtcg agctgctggc gctatttctg cgtaccggaa
    16261 cgcgcggtaa agatgtatta accctggcaa aagagatgct ggagaatttc ggctctcttt
    16321 atggcttgtt aacctctgaa tatgagcaat ttagtggcgt tcatggaatt ggcgtggcga
    16381 aattcgccca gttaaagggg attgccgaac tggcgcggcg ttactacaac gtacggatgc
    16441 gtgaagaaag ccctttactc agcccggaga tgacgaggga atttttacaa agccagctca
    16501 cgggcgagga gcgggagatc tttatggtga tctttctcga ctcccaacac agagttataa
    16561 cgcatagccg tcttttttcc ggcacgctaa accatgttga agtccatcct cgggaaatta
    16621 tccgcgaagc gataaaaata aacgcctcgg cgctgatcct tgcgcataat cacccttcgg
    16681 gttgtgctga acccagtaaa gcggataaac tcattactga acggataata aagagttgtc
    16741 agttcatgga tttacgcgtg ctcgaccata tcgtgattgg gcgtggagag tatgtttctt
    16801 ttgccgaacg cggctggatt taacccgcta tgcgcgatcc ttcgggatct ttgtctgttc
    16861 gggacttgag cacatcgctg agtcagcgta tactacgcca cctttgagaa tctcgggttt
    16921 ggcatttggg cctggcaatc gagagttcac atagaactgc gatgaccggg ctgtaaagcc
    16981 tgacgaggcg ccaatacccc atacgaagct cgagctaatt tgatttttgg agaatagaca
    17041 tgtcccgagt ctgccaagtt actggcaagc gtccggtgac cggtaacaac cgttcccacg
    17101 cactgaacgc gactaaacgc cgtttcctgc cgaatctgca ctctcaccgt ttctgggttg
    17161 agagcgagaa gcgttttgtc accctgcgcg tatctgctaa aggtatgcgt gtaatcgata
    17221 aaaaaggcat cgatacagtt ctggctgaac tgcgtgcccg tggcgaaaag tactaagtac
    17281 ttagaggaaa taaatcatgg ctaaaggtat tcgtgagaaa atcaagctgg tttcttctgc
    17341 tggtactggt cacttctata ccactacgaa gaacaaacgt actaagccgg aaaaactgga
    17401 actgaaaaaa ttcgatccag ttgttcgcca gcacgtgatc tacaaagaag cgaaaatcaa
    17461 ataattctcg ctttgatgta acaaaaaacc tcgccccggc ggggtttttt gttatctgct
    17521 tgcccccata ttgactgcat ctgttcattc ctggagatgc tatgcctgaa ttacccgaag
    17581 ttgaaaccag ccgtcgcggc atagaaccgc atctcgttgg tgcaaccatt cttcatgcgg
    17641 tggtgcgcaa cggacgcttg cgctggccgg tttcagaaga gatctaccgt ttaagcgacc
    17701 aaccagtgct tagcgtgcag cgccgggcta aatatctgct gctggagctg cctgagggct
    17761 ggattatcat tcatttgggg atgtctggca gcctgcgcat ccttccagaa gaacttcccc
    17821 ctgaaaagca tgaccatgtg gatttggtga tgagcaacgg caaagtgctg cgctacaccg
    17881 atccgcgccg ctttggtgcc tggctatgga ccaaagagct ggaagggcac aacgtgctgg
    17941 cccatcttgg accggagccg cttagcgacg atttcaacgg tgagtacctg catcagaagt
    18001 gcgagaagaa aaaaacggcg attaaaccat ggctgatgga taacaagctg gtggtaggcg
    18061 tagggaatat ctatgccagc gaatctctgt ttgctgcggg gatccatccg gatcggctgg
    18121 cgtcatcact gtcgctggca gagtgtgaat tgttagctcg ggtgattaaa gcggtgttgc
    18181 tgcgttcgat tgagcagggt ggtacaacgc tgaaagattt tctgcaaagt gatggtaaac
    18241 cgggctattt cgcgcaggaa ttgcaggttt acgggcgaaa aggcgagccg tgtcgggtgt
    18301 gcggtacgcc gattgtggcg actaaacatg cgcagcgggc aacgttttat tgtcggcagt
    18361 gccagaagta attgatgcga gccggatggc ataccatccg gcataaacgc tacgctaact
    18421 tcgccatcag cgcctgatgg acattctccg gcaggaaatg ggtgacatcg ccctgatggc
    18481 gcgccacctc tttcaccaac gatgaagaga taaacgacca ctctttcgac ggcataagaa
    18541 acacactttc cagttccggc attaagtggc gattcatatg cgccagctgc atttcatatt
    18601 caaaatccgc caccgcacgc aggccacgaa tcagcaccgt agcgtgttga ttacgggcga
    18661 agttcgccat caaatcacta aacccgacca cttccacgtt ccccagatgc gcggttgcct
    18721 gctgtgccag ctccacacgc tcttccaggg taaacatcgg ttttttactg gggctggcgg
    18781 caatcgccag aataacgtga tcgaacattt gcgtggcgcg cgtcacgata tcgatatgac
    18841 cattggtaat gggatcgaaa gtacccggat aaatcgcccg tttttgcata acaacctcaa
    18901 tgcgttttcg gtggcaggta aggttccagc agttgaagca gacgctgtag tgcgccctgg
    18961 ttttgataca gcacttcaac ggcatgacgg ccatagaaac tacggtaatc ggcgtcggtg
    19021 agtaaagagg aaacctcttt tgcaagcgta gtggcatcgg taacggtaat cagcccgctt
    19081 gcctgctcca gccgcgcgca aatgtcttta aagttaaacg tatgcggccc catcaatacc
    19141 ggaatagcgt gtgcggccgc ttccagcgga ttatgcccac cacgttcaac cagtgaaccg
    19201 ccaacaaagg cgagatcggc aatgccgtac agtaacatca actcgcccat cgtatcgcca
    19261 accacaacct gcgtgctggt ggaggggact tcccctgaag atcgtgtgat atagcttagt
    19321 ccagcctggc ggacaaggtt aatcgcatcc gggaagcgtt ctggatgacg gggtaccagg
    19381 atgagcaata aattcgggaa ttgctgtaac aatgcctgat gtgcggcgat caccacactc
    19441 tcttcgcctt catgagtgct ggtggcaatc cataccgggc ggtgtggtgc ccattggcgg
    19501 cgcagcgtca cggctttagc agccaactgc ggcgttacag aaatatcgaa tttcaggcta
    19561 ccggtaacgg tcacctgatt attttttgcg cccaacgcca caaaacgagc accatcttct
    19621 tcattttggg cggcaatcag cgtaatacga cgcagcaagc gacggacgaa tttacccagt
    19681 ttggcataac ctgcggccga gcgggcagag agtcgcgcgt tagcgatcac cagcggaatt
    19741 ttacgtttat gtagcgccgc aatcaggtta ggccatagtt cggtttccat aatcaacacc
    19801 agtttagggt cgactttatt caggaaacgg ttgagtgcat cgggcaggtc atacggcaga
    19861 taaacgtgct gaacatcctt cccgaaagcc gattgtacgc gctccgaacc ggttggagtc
    19921 atggttgtta cggtaatcgg taaatcagga taacgatgac gcagtgcgcg caccaacggg
    19981 atcgccgcca gagtttcacc gacggagacg gagtgcagca taatgccgcc tggttttagc
    20041 ggatggcggt aaaaaccgta acgttcaccc cagcgttttc gataggccgg agccttacgt
    20101 ccgcgcaccc agagccgtat ccagatcagc ggctgaataa ggtagagaag ggcggtgtaa
    20161 agcaattcga gcatagtaaa tagctgactt atggatgtgc aggggattct atgtatttag
    20221 ctgtggcttt accattactt ttcccatttt tgacttaaat agcaatttac ccgccaattc
    20281 ttgctgaaca tctatacttt ttttgtattt ttatgcgatt cattgatttc tgcagtacgt
    20341 gcaggaagga atcgcagagc aacagtaaaa gaatatggtt gctaatgatt ttatttgatt
    20401 gatttcttta ttgaaaatca acaatgtgct taaaaatcat tcgagtcgga ttgccgcgaa
    20461 agaagtgata cactagcgcg tcaatatcag tgcggtatca gtattcaggt agctgttgag
    20521 cctggggcgg tagcgtgctt tttctgcttc accttaatcg gataatctca acaaaaagag
    20581 ttacttgtgg ataagccatt tcgaagaatt ttgctcatta agatgcgttt tcatggggat
    20641 atgttattaa ctactcccgt cattagttcg ctgaaaaaaa attaccctga cgcaaaaatc
    20701 gatgtgctgc tttatcagga caccatcccg atcctgtctg aaaatccaga gattaacgcg
    20761 ctctacggca taaaaaataa aaaagcaaaa gcttcagaaa aaattaccaa cttttttcat
    20821 ctcatcaagg tattacgtgc caataagtat gaccttatcg ttaatcttac cgatcaatgg
    20881 atggttgcta tactggttcg cttattaaat gcccgtgtga aaatttccca ggattatcat
    20941 catcggcagt ctgctttttg gcgtaatagt ttcactcatt tggtgccgtt gcagggtgga
    21001 aatgtggtgg aaagtaactt atccgtgctg acgccattgg gacttgaatc gttggtgaag
    21061 cagacaacca tgagttaccc gcctgcaagc tggaaacgta tgcgtcgcga acttgatcac
    21121 gctggtgttg gacaaaatta tgtggttatc caacctacgg cgcggcaaat cttcaaatgc
    21181 tgggacaacg ccaagttttc cgctgtgatt gatgccttac atgctcgtgg ttatgaagtc
    21241 gttctgacgt ccggcccaga taaagacgat ctggcctgcg tcaatgaaat tgcgcaggga
    21301 tgccagacgc caccagtaac ggcgctggcc ggaaaggtga cctttccgga acttggtgcg
    21361 ttaatcgatc atgcgcagct gtttattggc gttgattccg caccggcgca tattgccgct
    21421 gcagttaata cgccgctgat atcgctgttt ggtgcgacag accatatttt ctggcgtccc
    21481 tggtcaaata acatgattca attctgggcg ggagattacc gggaaatgcc aacgcgcgat
    21541 cagcgtgacc gaaatgagat gtatctttcg gttattccgg cggcagatgt cattgcagct
    21601 gtcgataaat tacttccctc ctccacgaca ggtacgtcgt tatgatcgtt gctttttgtt
    21661 tatataaata ttttcccttt ggcggtttgc agcgcgattt tatgcgtatt gctcagacag
    21721 tcgccgcccg aggtcatcat gttcgggttt atacccagtc gtgggaaggc gaatgccctg
    21781 atgtatttga actgatcaaa gtgccggtta aatcgcatac caatcacggg cgcaatgcgg
    21841 aatattttgc ctgggtgcaa aaacatttac gtgaacaccc cgtcgataga gtcgttggat
    21901 tcaacaaaat gccggggctg gacgtttatt atgccgctga tgtttgttat gccgagaaag
    21961 tagcgcagga gaaaggcttt ttctatcgcc tgacgtcacg ttatcgccat tatgccgcct
    22021 ttgagcgggc aaccttcgaa cagggcaagc cgacacagct gctgatgctg acagataagc
    22081 aaatcgccga tttccagaaa cattatcaga ctgaagcgga gcgttttcat attctgccac
    22141 cggggattta tcctgatcgt aaatatagcc agcagccagc caatagccgt gaaatcttcc
    22201 gtaagaagaa tggaataacc gaacaacaat atttattgtt gcaggtcggt tcagacttca
    22261 cgcgtaaagg tgtcgatcgt tccattgaag cacttgcttc gttaccggat tcgctgcgcc
    22321 acaacacatt actatatgtt gttgggcagg ataaaccgcg aaaatttgag gcgctggcag
    22381 aaaaacgcgg cgtgcgcagt aatgttcact tcttctcggg gcgcaacgat gtctcggaat
    22441 taatggcggc ggcggattta ttacttcatc ctgcctacca ggaagccgca ggaattgtac
    22501 tgctggaagc gataactgca ggattaccgg tactaacaac agccgtttgt ggctatgcgc
    22561 attatattgt cgatgccaat tgcggcgagg ctattgctga gccattccgc caggaaacat
    22621 tgaatgagat tttacgcaaa gcgttaacgc aatcttcatt gcgccaggcc tgggcggaaa
    22681 atgcgcgaca ttatgctgat acacaagatt tatacagtct gccagagaaa gcggcggaca
    22741 tcataacggg tggtctggat ggttgaactt aaagagccgt ttgccacgtt atggcgcggt
    22801 aaagatcctt ttgaggaagt taaaaccttg cagggtgagg tatttcgtga actggaaact
    22861 cgccgcactc tgcgctttga aatggcgggc aaaagctatt ttctcaaatg gcatcgcggc
    22921 acgaccctga aagagataat caaaaattta ctctcattgc ggatgccagt attaggcgca
    22981 gaccgcgaat ggaatgcgat tcatcgactg cgggatgtcg gcgttgatac tatgtatggg
    23041 gtggcattcg gcgaaaaagg cattaatccg ctgaccagaa cctcgtttat tattaccgaa
    23101 gatctgacac caaccatcag tctggaagat tactgtgctg actgggcgac caacccacca
    23161 gatgttcgcg taaagcgtat gcttattaag cgtgtcgcga cgatggtgcg cgatatgcat
    23221 gctgcgggca ttaaccaccg tgactgttat atctgtcatt tcctgctgca cttgcctttt
    23281 tccggtaagg aagaggtgtt aaaaatttca gtaattgacc tgcaccgggc gcagatacgt
    23341 gcaaaagtac cgcgtcgttg gcgggataaa gatcttattg gactttattt ttcttcgatg
    23401 aatattggcc tgactcagcg ggatatctgg cggttcatga aggtgtattt ttctgccccg
    23461 cttaaagaca ttctcaagca ggaacaaggg ctgctgtcgc aagcagaaga aaaagccaca
    23521 aaaatcaggg aaagaacgat tcgaaaatcg ttgtaatttc cggagagaaa tatgagtgcc
    23581 cactatttta atccacaaga gatgatcaat aagacaatca tcttcgatga aaggccagcg
    23641 gcgtcagtgg catcatcatt ccatgttgct tatggcattg atcagaactt tctttttggt
    23701 tgtggtgttt caatcacgtc agttttgtta cataacaacg acgtgagttt tgtcttccac
    23761 gtttttattg atgatatccc tgacgccgat atccagcgtt tatcccaatt ggcgaaaagc
    23821 tatcatacct gtatccagat ccatctggta aattgtgaac ggcttaaggt attaccgacg
    23881 accaaaaatt ggtctattgc catgtatttc cgttttgtaa ttgcagatta ctttattgat
    23941 caacaagata agatcctgta cctggatgct gatatcgcct gtcagggaac cttaaaaccg
    24001 ctgataacaa tggatcttgc caataacgtt gctgctgttg ttactgaacg cgatgctaac
    24061 tggtggtcgt tacgggctca aagtctgcag tgtaatgaac ttgaaaaggg ttactttaat
    24121 tcaggtgtcc tgttaattaa tacactagcg tgggcgcagg agtccgtttc tgctaaagcg
    24181 atgtcgatgc ttgctgataa agccgtcgtt tcccgtttaa cctatatgga tcaagatata
    24241 ctaaatctta tcctgttagg gaaagttaaa ttcattgatg ctaaatacaa tacgcaattt
    24301 agtttaaatt atgaattaaa aaaatcattt atttgtccaa ttaatgatga aaccgtatta
    24361 attcattatg tcggcccgac aaaaccctgg cattactggg ccggttatcc aagcgcgcag
    24421 ccatttatca aagccaaaga agcatcgccc tggaaaaatg aaccgttaat gcggccaatg
    24481 aactcaaatt atgctcgtta ttgcgctaag cataattt
//
LOCUS       DAZFRG010000049        24502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFRG010000049 DAZFRG010000000
VERSION     DAZFRG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.049"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..831
                     /gene="ynaI"
                     /locus_tag="ACHKUC_001560"
     CDS             <1..831
                     /gene="ynaI"
                     /locus_tag="ACHKUC_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO6617836.1"
                     /translation="YKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRD
                     ITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFS
                     GIMLYFDRPFSIGDWIRSPDRNIEGTVTEIGWRITKIKTFDNRPLYVPNSLFSSISVE
                     NPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSS
                     LNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPDQGR
                     "
     gene            complement(882..2495)
                     /gene="mppA"
                     /locus_tag="ACHKUC_001561"
     CDS             complement(882..2495)
                     /gene="mppA"
                     /locus_tag="ACHKUC_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO6617837.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2832..3731)
                     /gene="pgrR"
                     /locus_tag="ACHKUC_001562"
     CDS             complement(2832..3731)
                     /gene="pgrR"
                     /locus_tag="ACHKUC_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO6617838.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3869..4789
                     /locus_tag="ACHKUC_001563"
     CDS             3869..4789
                     /locus_tag="ACHKUC_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6617839.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTVGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            4860..5498
                     /locus_tag="ACHKUC_001564"
     CDS             4860..5498
                     /locus_tag="ACHKUC_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6617840.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAANAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            5789..6517
                     /gene="mpaA"
                     /locus_tag="ACHKUC_001565"
     CDS             5789..6517
                     /gene="mpaA"
                     /locus_tag="ACHKUC_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO6617841.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPTPATSHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(6492..7457)
                     /gene="ycjG"
                     /locus_tag="ACHKUC_001566"
     CDS             complement(6492..7457)
                     /gene="ycjG"
                     /locus_tag="ACHKUC_001566"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO6617842.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTRQELQKILPAGAARNALDCALWDLAARK
                     QQQSQADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            7576..8082
                     /gene="tpx"
                     /locus_tag="ACHKUC_001567"
     CDS             7576..8082
                     /gene="tpx"
                     /locus_tag="ACHKUC_001567"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO6617843.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(8126..9667)
                     /gene="tyrR"
                     /locus_tag="ACHKUC_001568"
     CDS             complement(8126..9667)
                     /gene="tyrR"
                     /locus_tag="ACHKUC_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO6617844.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(9815..10876)
                     /locus_tag="ACHKUC_001569"
     CDS             complement(9815..10876)
                     /locus_tag="ACHKUC_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO6617845.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(10873..12270)
                     /locus_tag="ACHKUC_001570"
     CDS             complement(10873..12270)
                     /locus_tag="ACHKUC_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO6617846.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            12427..13425
                     /locus_tag="ACHKUC_001571"
     CDS             12427..13425
                     /locus_tag="ACHKUC_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6617847.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDIDNYGDSIALTDALIESGHKNIACLHAPLDVHVSVDRVNGYRQSLAMHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(13476..14381)
                     /gene="ompG"
                     /locus_tag="ACHKUC_001572"
     CDS             complement(13476..14381)
                     /gene="ompG"
                     /locus_tag="ACHKUC_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO6617848.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETLALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(14426..15508)
                     /locus_tag="ACHKUC_001573"
     CDS             complement(14426..15508)
                     /locus_tag="ACHKUC_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6617849.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAREVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(15522..16181)
                     /gene="pgmB"
                     /locus_tag="ACHKUC_001574"
     CDS             complement(15522..16181)
                     /gene="pgmB"
                     /locus_tag="ACHKUC_001574"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO6617850.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(16178..18445)
                     /gene="ycjT"
                     /locus_tag="ACHKUC_001575"
     CDS             complement(16178..18445)
                     /gene="ycjT"
                     /locus_tag="ACHKUC_001575"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO6617851.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLTGLYHRAGKGEINELVNLPDVVGMEIAINGEIISLSREAWQRELNFASGELR
                     RNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLPAGKTLTLQKRVWIDWRDDRHVALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLLGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHESMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELLLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSSPLFWQGCELQVTLDVQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGNF
                     ILPFNGTATTHQEGE"
     gene            complement(18442..19497)
                     /locus_tag="ACHKUC_001576"
     CDS             complement(18442..19497)
                     /locus_tag="ACHKUC_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6617852.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWSVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(19507..20295)
                     /locus_tag="ACHKUC_001577"
     CDS             complement(19507..20295)
                     /locus_tag="ACHKUC_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6617853.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGVKQIERILEALAEVGGKGVVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQAAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWVRTC"
     gene            complement(20314..21366)
                     /locus_tag="ACHKUC_001578"
     CDS             complement(20314..21366)
                     /locus_tag="ACHKUC_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO6617854.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILASEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(21397..22239)
                     /locus_tag="ACHKUC_001579"
     CDS             complement(21397..22239)
                     /locus_tag="ACHKUC_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6617855.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIIMLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(22226..23107)
                     /locus_tag="ACHKUC_001580"
     CDS             complement(22226..23107)
                     /locus_tag="ACHKUC_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6617856.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTTLVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVLLSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(23128..24420)
                     /locus_tag="ACHKUC_001581"
     CDS             complement(23128..24420)
                     /locus_tag="ACHKUC_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6617857.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKSEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQTSQKKLDELVEQR"
BASE COUNT         6090 a         6561 c         5863 g         5988 t
ORIGIN      
        1 tataaactca aatttatcac cccaggcgtt atcgatttta tctgtacgtc cctgattgcg
       61 gttattctga cgattaagct atttctgctg attaatcagt ttgaaaaaca gcagattaaa
      121 aaaggtcgcg atatcaccag tgcgcggatc atgtcgcgca ttatcaaaat caccattatt
      181 gtggtgcttg ttctgcttta tggcgaacat ttcggcatga gcctttctgg cttgttgacc
      241 tttggtggta ttggtggtct ggctgtcggt atggccggta aagatattct gagtaacttc
      301 ttttccggga ttatgctcta tttcgaccgt cctttcagta ttggcgactg gatccgttca
      361 ccggacagaa atatcgaagg tacagtaacg gaaattggct ggcgaattac caaaattaag
      421 acctttgata atcgtccatt gtacgtaccg aactcgctgt tttcgtcgat cagcgtagaa
      481 aatcctggac gaatgaccaa ccggcgcatt accacgacca ttggtttacg ttatgaggat
      541 gcggcaaaag tgggcgttat tgtcgaagcc gtacgtgaga tgctgaaaaa tcacccggcc
      601 atcgaccagc gacaaacctt actggtttat ttcaaccagt ttgctgattc gtcattaaat
      661 attatggttt attgctttac caaaaccacg gtatgggctg agtggcttgc cgcacagcaa
      721 gacgtttatt tgaagattat cgatattgta cagtcacacg gcgcggactt tgccttcccc
      781 agccagacgc tgtatatgga taacattaca ccgccggacc agggtcgcta aatcagacat
      841 cactggcgtt atccacaagg gtaacgccag ttcccatcac attaatgctt cacaatatac
      901 atagtccgac tgtacgccac atcttcaggg ttattaatgg gataaccttt cagccacggc
      961 ttgattaatc gtccattggt atattgataa attggtgcaa tcggtgcttg ctccatgagg
     1021 attttttctg ccgcgttgta atcggcatta cgcgctttaa cggtattttc cgtcgatgcc
     1081 tgggccagaa ctttgtcata tgccggattg ttaaagcgtg aaatattccc ggaatgcgtt
     1141 gacgttaata atgtcaggaa agtggagggt tcattataat cccctaccca cgaggcgcgg
     1201 ataacatcaa aattgccggt attacggcta tcgatatagg ttttccattc ctggttttgc
     1261 aatttaacat ctacgcccag gttctttttc cacatcgatg ctacagcaat cgcaattttt
     1321 tgatggtttt ctgaagtgtt atacaaaagc gtcaacttca gcggtttttg cggaccataa
     1381 ccagctgcgc tcaacaaagt ttttgcctgc gcattcagtt cttcctgact catttgttca
     1441 aacggcgaag gttccggcgt aaatcccgcg gtaacatctg gtgtaaaatg ccacgctggc
     1501 ttttcgcccg tccccaacac tttttcggtc atcaggcggc gatctatcgt catacttaat
     1561 gccaggcgaa cgcgctgatc tgccgtcggc cctttttgcg tgttaaacgc ataataatag
     1621 gtcccgagct gcggcggcgt ataaacctgc cccggaatat ccttcaacaa cttctgatac
     1681 atatttttcg ggaaggattc ggtgatatca atatcccccg cgagataacg cttagtggct
     1741 gcggattcct gattaattgg caggaaggtc actttttgca gtaccgtttt ggcgttatcc
     1801 caataatggg tattcggcac tacgaccagt ttttcattga ctacgcgctc tttaagaaca
     1861 taagcgccat tgccgatcag atttccgggt ttcgtccact ctttactgct ttctacgttg
     1921 gctttttgca ccgggaagaa ggcaaagtta gcggttaaat tcacaaacca cggcaatggt
     1981 ttatcaagct ggattttcaa agtatgggca tcaactgcgg tgacgccaag ctggtcaggc
     2041 gcggctttac catcaataat cgcctgtgcg ttgttgattc ccgccagcgc ggcaaaccat
     2101 gcaaatggcg atagcgtttt cgggtccacc agacgttgcc agctgtagac aaaatcttgc
     2161 gccgttaccg gtgtgccatc cgcccatttt gcgttatcgc gcagggtaaa agtccagata
     2221 cggttgtcat tacttttcca ctgagtcgca acgccgggga caatctcccc tttctcgttc
     2281 tgattcacca gtccttcaaa cagatcgcga atgacctgaa tctccggcag gcccacggct
     2341 ttagcgggat ccagcgacgc tggctcatct ttaatgtggc gcaccagctc ctgcttctct
     2401 gccagtactg tgccgctcgg aacttcagca gcatacgaaa gagaaatgct gctgaccaac
     2461 agcgcacaac acgtgactga aacagagtgc ttcataagtt tccctctaac cgacattaaa
     2521 attgatgcgt aattatttgt ttcgctttaa agaaatgcaa acaacttcca tcaaaaaagt
     2581 gacaaaggaa gcatcaaatg ttttaagtat caatatatca aatcagattt agtgtaaata
     2641 atagtctcca aaatgcggta aagcacatag aactccgaac actacgttcg ggcttcgcac
     2701 attcaaaccc gttttcggta tcataatcag catctgtcgc cattgtgcga agaataaaaa
     2761 ataaccaaaa cgttgaaagg aactgagaat acgttaagag aagaattaaa ttaatgtatc
     2821 agcaagtagt gctaaacagc acctttatac ttcagcctat ctataaataa tgagaatgcc
     2881 gagccagcat gtcgacggtg tggatagtac aggtgataac cgggtaaatc tggtgtgaat
     2941 ttatccaaaa cacgtatcag ttttttttct ttaatagccc gctcaacctg atcataaggt
     3001 agatacgcca atccatgccc atcaattgca gcatcaatga tcaggtctat cgtattcagt
     3061 aaaagctgac cttccatgcg aacacgaact tcacgtccac cgcgtattaa tctccagcga
     3121 tttgctgtac ccgatgtggg aagatacaaa ttaattgcct gatgatctat taattgtgac
     3181 actgacgttg gagcacttcg acgagaaaaa taatccggtg agccaacaat agccattggt
     3241 atgtctggcc cgattcgaat ggcgatcata tctttatcca tctccccacc cagacggacg
     3301 cctgcatcaa aacgttcaga aacgacatcg gtcaaaccat aatcaatggt aagctgaata
     3361 tcaatttcag gatgcgattt caggaatgtg cgcatagctg gtaacaatat cgtttttgct
     3421 gcatgttcta cagtagtaat acgtattgtc ccggatgggc ggttctgcag atcgctcagg
     3481 gatgtcaggg cagaatctat gtcatgcaac atagggccaa gaacagacaa aagatgctcg
     3541 cccgcttcag tgggtacaac gctgcgcgtg gttcgcgtca gaagccgcaa tcccaatcgt
     3601 tcttctatac ggcgcactat ctggcttaaa gctgactgcg ccatgctcag gcgggctgct
     3661 gcgcgagtga agctacgctc ctctgcaaca acgacaaacg ccatcagatc agcgatttct
     3721 tcacgcttca ttcagtatct ccatacaatc tattaatcat ttaaatgata ggtctaatca
     3781 tgattttggg tctaatcaac ttaatgatca atatctatag tgatatcacc aaccgttaaa
     3841 acgaaccgac aactccatga ggaacggaat gatgaataat aaagtcagct tcactaacag
     3901 caataatcca accatctctt tgtctgcagt gatctatttc cctccaaaat ttgatgaaac
     3961 ccgtcagtat ccggcaattg tggtatctca cccaggtggg ggcgttaaag agcaaacggt
     4021 cggaacctat gccgaaaaac tggcggaaaa gggatttgtc actattgctt atgacgcatc
     4081 ttatcaaggt gaaagcggcg gcgagccgcg tcagttagaa aacccataca ttcgtactga
     4141 agacgtcagt gccgtgattg attacctgac aacgctttct tatgtcgaca atacccgaat
     4201 tggtgcgatg ggaatttgtg ccggtgcagg atatacagcc aatgctgcaa ttcaggatcg
     4261 ccgcatcaag gccatcggta ctgttagtgc ggtcaatatt ggttcgatgt tccgtaatgg
     4321 ttgggaaaac aatgtgaaat ctattgatgc cctaccatac gttgacgcgg gttcaaacgc
     4381 ccgaaccagc gatattagca gcggagaata cgccactatg ccactggcac caatgaagga
     4441 gtccgatgcg ccgaacgaag aactgcgtca ggcctgggaa tattaccaca cccctcgcgc
     4501 gcagtatcca acagcaccgg gttacgctac tttgcgcagc cttaaccaga ttattaccta
     4561 tgatgcttac catatggcgg aagtgtacct gactcagccg atacagattg tggcgggcag
     4621 ccaggctggg agcaaatgga tgagtgacga cctgtacgat cgagcatcaa gccaggataa
     4681 acgctatcac attgttgaag gcgcaaacca tatggatctt tatgatggca aagtctatgt
     4741 ggctgaagca atttcagtat tagctccgtt ctttgaggaa actctgtaat gaaacatatt
     4801 caaatccgta atagtgatat ggcctggcat attgctgcca ataatctggg aaaagtttca
     4861 tgaaaaatgt actgattctt ggtgctggcg gtcagatagc ccgccatgtg attaatcaac
     4921 tggcagataa acaaaccatt aaacaaactt tatttgcccg tcagccagca aaaatccaca
     4981 aaccctatcc cactaatagc aagattatta tgggggatgt actgaatcat gcagcactga
     5041 agcaagccat gcagggacag gatgttgtgt atgccaacct tacgggggaa gatttagata
     5101 ttcaggcaaa ttcagtgatc gctgcgatga aagcctgtga tgttaaacgt ctgatctttg
     5161 ttctttccct cggtatttat gatgaagttc caggaaaatt tggcgaatgg aataatgcag
     5221 ttattggtga acccctaaaa ccattccgca gggctgcgaa tgcaattgag gcttctggac
     5281 tggaatatac aattcttcgc cctgcctggc tgactgatga agatattatt gattatgagc
     5341 ttacctcacg caatgaacca ttcaaaggta cgatcgtatc acgtaagagt gtcgcagcat
     5401 taatcacaga tatcatcgat aaacctgaga aacacattgg tgagaatata ggcatcaatc
     5461 aacccgggac tgatggtgat aaacccttct tcatgtaaat caattatata gctaccatcg
     5521 tactgtacta tgtgcagtac gatttattaa acttattaag gtgaactgat caatcagatg
     5581 cgattaacat gtgtagaacc agtaagaata aaatacgcca tgtatataac attacgacaa
     5641 ttctaatatc tgcacatcaa tgaaaataca aacaacttcc ggcaaaaaag tgatacaaga
     5701 tcgatgacat agcgttgaag aaaaggattt gataatccgc aaaatttgca caacactgcc
     5761 tgtaatactt cctgaaccag agacgatcat gaccgtaact cgcccacgcg ccgaacgcgg
     5821 cgcatttccg cccggaacag aacattacgg acgttcatta ttgggcgcgc cgttaatctg
     5881 gtttccgacc cctgcaacca gtcacgaaag tggtttgatt ctggctggca ctcacggtga
     5941 tgaaaactct tcgatcgtca cgctctcctg tgctctgcgg actttgacac cttctttgcg
     6001 ccgccatcat gtggtgctgt gtgtgaatcc tgacggctgc cagttagggt tacgggccaa
     6061 tgctaatggt gtggatttaa accgaaactt tccggcagcg aactggaagg aaggtgaaac
     6121 agtttatcgc tggaacagcg ccgctgaaga acgtgatgtc gttttactga caggtgacaa
     6181 acctggctcc gaacctgaaa cccaggcgct gtgccagctt atacaccgca ttcaacctgc
     6241 ctgggtggtc tcattccacg atccgctggc ctgtattgaa gatcccagac acagcgaatt
     6301 aggtgaatgg ctggcccagg cgtttgaatt gcctctggta accagcgtcg gttatgaaac
     6361 gcctggctct ttcggcagtt ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt
     6421 tccacccatc tcctccgacg aagccagcga aaaatatctc tttgcgatgg caaacttgct
     6481 gcgctggcat cctaaagatg caattcgccc gtcgtgaact gaagcgccgg ttccacatct
     6541 accgccagcc aggtcggtcc gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa
     6601 gcggcgctaa tggcacgaga ggtacacaac atgcagccca gcatcagacg aaaaccttgt
     6661 gcacgcgctt cagtcgccag cgccagcgct tccgtcagac caccggtttt atcaagctta
     6721 atgttaacca tctcatagcg ccctttcagc gccttcagat tgctacgagt atgacaactt
     6781 tcatcagcac aaatcggtaa cggatgaata aaattctcca gcgccgcatc gtcctgcgca
     6841 ggaagcggtt gttcaagcat cgcaacgcct aaatctgcca atagctggca acgcgccgcc
     6901 aacccttctg cacgccagga ttcatttgca tcaacgatca gcgtcgcatc gggcacagct
     6961 gtgcgaattg ccaccatccg ctcactgata agatggttat ccagcttcac tttcagtaat
     7021 ttcgcgcctg cctgccagag tgttgatgca ctattggcca tctgatcagg cgtaccgatg
     7081 actatcgtct gtgccgtggt gactgtctcg ggaagcgtta tgccgatcaa atcagcctgc
     7141 gattgctgct gttttcgcgc cgccagatcc cacaatgcac aatccagcgc attacgtgct
     7201 gcgccggcag ggaggatttt ttgcaactcc tgccgtgtca gccctttctc taattgcggc
     7261 acgacgctca taatttgcgc cattaccgag gcatcacttt ccccataacg cggatacggc
     7321 gtgcattcgc cggtgccttt aataccctct tcttccagtt caaccaccac cacgcgcgct
     7381 tcactgcgac ttccccgagc aatcacaaac ggggtatgta agggccaggc ctcctcaaat
     7441 accttaacgg ttctcatctt tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa
     7501 tagcgtttgc tgtgttattg acagttagca taaactaggt gtgacgttaa ctatatgtaa
     7561 acaggaaaga taatcatgtc acaaaccgtt catttccagg gcaacccggt tacagtcgcc
     7621 aattccatcc cgcaggcggg tagcaaagcg cagactttta ctctcgtggc aaaagatctg
     7681 tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt
     7741 attgataccg gtgtttgcgc cgcatcagta cgtaagttta accaactggc aaccgagatc
     7801 gacaacaccg ttgtgctgtg tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc
     7861 ggcgcagaag gtctgaacaa cgttatcacc ctctccactt tccgtaacgc tgaattcctg
     7921 caagcctacg gtgtggcaat tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt
     7981 gtggttattg atgaaaatga caatgtgatt ttcagccagc tggtggatga aatcaccacc
     8041 gagccggatt acgaagcagc tctggctgta ctgaaagcat aagttaatat gcctgatggt
     8101 gttgcaccat caggcatatt cacgcttact cttcgttctt cttctgactc agaccatatt
     8161 cccgcaactt attggcaatc gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg
     8221 tgctgggata attgcgataa agctgggtta ataccgagcg ttcaaaacgg ctggtgattt
     8281 cgtccagcga accttccatc gcatcttcgc ccacggctac cgttgcggcg tcataatccg
     8341 gcaacaaaat atcctgtgga cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga
     8401 tagcgttctt taactgccgc acatttcccg gccacgcata acgcgtaagt acagtattca
     8461 gatcagcggc cagtttcgga cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca
     8521 gctcggttaa cggcatgatg tcctgcggac agtcacgtag cggcggcaga ttgagcgtca
     8581 acacgttcag acgataatag agatcttcac ggaacacgcc tttttgcacc agttcgacca
     8641 gattcttctg cgtagcacaa atcacccgca catcgacatg cacctcatgg tcttcgccaa
     8701 cccgacggaa ggtgccatca ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca
     8761 tctcacctat ttcatccaac agcaccgaac caccgttcgc ctgctcaaag aaccctttct
     8821 tcccttccgg agcatgacca aacagttcac tctcgaccgc atcttccggt atagacgcac
     8881 agttcagcgc caggtaaggt ttgctcgctc tggggcttgc ctgatgacag gcatacgcaa
     8941 agagatcttt tccggtgccg gtatcacccg taatcagcag cggcgcgctt agcatcgcca
     9001 gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacagcgaca atttgactga
     9061 aggcgctgac atcctgggcg gcgacatttt gcaactgacg gcccatacga atcgttgatc
     9121 gcagcatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag
     9181 gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta tgcgaatctt
     9241 gcggttcgct ttccagccag cgtaaaaaat taaagccgtt aatcaattgc gcggcggtat
     9301 ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca gctcgccggg ttcgccatat
     9361 ccactttgct tttcatatcg acagagagca caggttcagg caacgcctcc agtaacgcgc
     9421 tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac
     9481 ctgcaatacg gcgtatttcg gccatcagac tgctgaaact ctcaaactcc agttcagcaa
     9541 aattgaggta gattcgccca atgggatcaa tctcaatacc gcgtaaatca atgcctctta
     9601 gcacgagtag atcgagtaat tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca
     9661 gacgcatggg aaccttcacc tgaaaaaaga acaattaata tgatgatatg acactatttg
     9721 aatagcagga aggtttctgt caacaatcat tgacacaaag acggaaaaac gggcacttcg
     9781 gcgtaaagat atccggtcag cacccgatat tgcattattt ttcgctgggc gttttgcctt
     9841 tttgcagagt ttctttcacc tgaccgataa gctgacgacg gaaatccccg aggcgaggtt
     9901 tgtcatcgtc aatccacggc agcgggcggc aaagctccat agctttaatc cccagtcgtg
     9961 ccgtcagaag tccagcacca atcccctgag ctgcgcgggt agacaaacgc gcagcgagat
    10021 cttgcgacat ccagtccatc cccacttcgc gcaccagttc gctggctccg gcgaaagcga
    10081 tattcagcaa taccagctta aacaggcgca aacggctgta atatcccagt tcaatgccat
    10141 acagcgtggc gatgcgatta atcaaacgca gattgcgcca ggcgataaac gccatatcca
    10201 ccagcgccag tgggctaacg gcaatcatta acgttgattc cgccgctgag cggctgattt
    10261 cgcgccgcgc ctgggcatct aaaactggct ggaccaagtg agcatacagg ctgaccactt
    10321 cacgatcgtt ctgcgtttca tggattgagg cataccagcg ctgcagcgcc ggatgtgact
    10381 gatcaatacc cgcctgctgc gccagttttt cgcagaatgc acggccttta cccgtgccgt
    10441 gactgtgcaa caaatcgcgc gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata
    10501 agcgccgcca ctctgttacc acagaaccca cgccagcgcc gataatcaat gccccagcgg
    10561 cacatccacc cagcgccacc cagtcctggg tttgccaggc gttcattgtc cactgcacgc
    10621 cctgcccgac aacgcttgcg ccaaacagag ccagcccgcc catcaccatt ttgcgccaca
    10681 ggctgcgttt cggacgtaac gctgcatcca ttaccgcttc aacttgcccc tcttcttcct
    10741 gcgcttcatc gagcgtggct ggggcaaaat tttgcgcctg attttcgtca aaggtctgct
    10801 gcgccctgaa ttttggattc tgatcgacct ccagtggacc gtcgaaatca atacgtggtt
    10861 ttaacggttc ggtcatcgca atttatctcc tattaaaaat tccagcgcag catcaagacg
    10921 aatatgcggt agcggtttgt cgacatccat cacctgcgga cgaaacgcct caaactggaa
    10981 gccttgctta tcccagaacg cctgacctgg caaacgtgcg ggaacttcgc caggataaac
    11041 agtgagcggt gcgccatcgc tgagtcgatt accgcgcagc gccgggattt tctcaccgtt
    11101 aacatcaata attccgctgg tggtcgcctg aacagacgcc agccccagac agtccatact
    11161 gatcccttcg aacgccgcat tttgccaggc atcctgaatc agttgttgca gcaatgaaac
    11221 catattggcg tgctgatcga tggtcacatg gtccgcttta gtggcagcaa acaatagctt
    11281 atcgataacc ggcgaaaaca aacgccggaa cagggtacgc tgcccgtagt ggaaactttg
    11341 catcagctgc gtcagcgcca gacgcatatc attaaatgcc tgtggtccac tgttgagcgg
    11401 ttgcaggcaa tccaccaaca caatctggcg gtcaaagcgc agaaaatgat tcttatagaa
    11461 ccccttcacc actttctcgc agtaataatt aaaccgctcg cgcaacattc cggcattggt
    11521 gtgcttatcg gcctgcgcca gtttggactc gccccaggca tcgacatctg gccatgggaa
    11581 gaattgcagc gcgggcgctc ctgccatatc gccaggcaaa acaaagcgtc ctggctgaat
    11641 aaagtgcagc ccctgctgtt tgcagtgatg gagataatcg gtccacgcgg cggcaatgtc
    11701 tgccagccgg ttttcgtcgg caggcgctag cgggtccagc ccttcgcaca tcattcgcca
    11761 tttcgccgac cattcgccgc gctgaccatt gagtaagccc gtcatctggc gcgaccagct
    11821 taaatagtcc tgcgccagca tcggcaggtc gagcaaccat tcgccgggat aatccacaat
    11881 ctcaagatac agcgtggagg tgtccttaaa gtggcgcagc agcgaatcgt tcgatttaaa
    11941 acgtaacgcc aggcgaattt cactgacgcc gcgcgtaggc gtcggccagg cgggaggatc
    12001 gccatataac tgcgccaacc cttcatcata ggtgaagcgc ggaatgccaa agtcacgctg
    12061 gggaatgcgt tttacgccca gcaggcgctc ttcacgcacc gcacttaaaa gcggcaaacg
    12121 tgctccggca tgaatattga gcaactgatt aaccatcgcg gtgataaacg ctgttttgcc
    12181 gctgcggcta agtccggtta cagcgaggcg cagatgtctg tcgacacccc gattcaccag
    12241 cgcattgagt tcatttttaa gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa
    12301 aaaaaaatcg ttcgtaagga tatgaatgtg ggggccaggc cattgattat caactacccg
    12361 tgttgtcatc gacgggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc
    12421 tgacctatgt cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc
    12481 gtatcacgcg tgctgaataa gcaaaccaat atctccccgg aagcgcgcga aaaagtgtta
    12541 cgggccattg aagaattaca gtatcaacca aacaagctgg cccgcgcgct gacctcttcg
    12601 ggttttgatg ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg
    12661 tttttctctg aagttttgca tgccatcacc gccaaagcgg aagaagaagg tttcgacgtg
    12721 atattacaaa cgtcgcacaa tccggcagaa gatctgcaaa aatgcgaaag caaaattaag
    12781 cagaaaatga ttaaaggcat tattatgcta agttcgcctg ctgatgaatc cttctttgca
    12841 caactcgaca aatacgagat cccggtggtg gtgatcggta aagttgaagg tcaatacagc
    12901 catgtttatt ccgtcgatat cgataattat ggcgacagta ttgcactaac cgatgcgtta
    12961 attgaaagcg ggcataagaa tattgcatgc ctgcatgcgc cgcttgatgt tcatgtttca
    13021 gtggatcggg taaatggtta taggcaaagc ctggctatgc ataatattgc agtgcgtgat
    13081 gaatggattg ttgacggcgg ttatacccat gagacagcat taaaagcagc acgggaatta
    13141 ttaagccagt caccgttgcc tgaggcagtg tttgctactg acagcctgaa attaatgagt
    13201 atttatcgtg cggcggcaga gaaaaacatt gctattccgc agcagttagc ggtggtgggt
    13261 tatagtaatg aaacgctgtc gtttatatta acgcctgcac caggcggcat cgatgttccg
    13321 acgcaggagt taggtcaaca aagctgcgag ttattattcc agctaattgc cggaaaaccg
    13381 tcaccacaaa atattaccgt tgctacgcat atgtcgttga aataaatata tgccggattt
    13441 ggcgctcgcc ccgcatccgg caatttagca cattatcaga acgagtaatt caccccaaca
    13501 cccgcataat ggaatttatc gctgtcgcct tcgtcgtgat cctgccactc aaacgcgtat
    13561 tccagcgaaa cggaaagacc attctggaaa tcataaccgt aaaataaacc cacacggtta
    13621 aaatcatggc cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc
    13681 ccaatgcgcg tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga
    13741 atttcttgcg tggaaaactc accgttattg cggctatcat ccatattgaa accacgctcg
    13801 agataatagt tcactcgcaa ggcaagcgtt tcgttgaagg tatattgcag acctgtttcc
    13861 gtttcgacac gggtatcagc ataaccggtc gtattcagat cgttggcaaa tttatacatc
    13921 gacagccaac cgttgaaacg taaatcgtca gtcagtttca catcccagtc tggcgcaatt
    13981 ttccagcgct gcatattcgc cgtgtcttta cccggttcat caacgtagtg ataaccataa
    14041 ttacggaaac caccagtcag accgaaactg aaatcatcgc tttcgaggaa ctgataatgt
    14101 acctccagct ccgggcgatc aaaccacgtt ccacgtttac ccgcgctata atctaccggc
    14161 ccttcctgat aataggccag agaaattctc cacggcccgt tggcggcatt aaaatagaca
    14221 gaaggctccg ccagcccatc catatcttcg ccataaccct cgacgttttc tatttcgtac
    14281 atcgcgccga tattaaagtg ccagtcgttc ttttcctcgg cctgtgcgca ggccattccc
    14341 gcacacatca ccagtgcggt acagggtaat aactttttca ttattgttcc ttgtattatc
    14401 ctgggagaat gccttccccc tgtatttagc aaattgctat ttccgtttct gcatcaaaga
    14461 aatgacattt agtcatatca aaatgaatag tgatattttc tcctgcatga taatcattta
    14521 acgcaccagc acggaccact aactcgtgcc ccccaaccgt ggtgtagagc ataaattccg
    14581 caccggttaa ttccgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg
    14641 gatgaatatc ttccgggcgt attcccatta cgatgggctt atgcaaactt tcctgtgttt
    14701 tcagaaccgc taatttctct tcgggtatgg ttaatttaag cgtttccgta acgaatttat
    14761 cgccatcgat cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa
    14821 acatattcgc aggttggtta taaaccgttt tcggcgcacc gacctgctga acaatcccat
    14881 ccttcataat gacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt
    14941 agatcatggt ggtgttcagt ttctgatgca gcttgctgat ctctgcgcgc atttgcacgc
    15001 gcagcttggc atccaggtta gagagcggct catccattaa aaacacgccc gcttcgcgca
    15061 caatcgcccg cccaagcgcc actcgctgac gttgcccgcc ggaaagcgcg cccggcttgc
    15121 gtttcaggta ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca
    15181 cctctctggc gattttttgc atcttcaggc caaacgccat gttgtcgtag accgtcatat
    15241 gcggatacag cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt
    15301 cattcattcg tttgccgtcg atcaacagat caccgccgct gatctcctca agcccggcaa
    15361 tcatacgcag ggtggtcgat ttaccgcagc ccgacggacc gacaaacacg atgaactctt
    15421 tatcggcaat ctccaggttg aagtccttca ccacatgcac ctggttatcg tagatctttt
    15481 gaatatgttg taacgaaagc tgtgccatgt tgattccttt gctatacgtt ttgccagaag
    15541 gccgataacc gcggccaggt cagggagtcc gtcgagggca acaataattg cgccccggtt
    15601 aagcccgcgc cgatccccac cgagcgcata ccgctggcgt taatggcttc aatgcccgcc
    15661 tgcgcatctt caatgccgat acatgcctgt ggcggcacgc ccagccctgc acaggcggcg
    15721 agaaagattt ccgggtccgg tttcgaattt ttgagttggg aagcgtccgc gcagaaggtg
    15781 aaaaactcgc gcagctccag cgctgctaaa atcgtcggcg cattcaggga gacagaagcc
    15841 agcccaaccg gaatctgctg tgctcggaga tctaccagca aattgtgaat gccaggcagc
    15901 acagcattaa ccgtcagttc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc
    15961 agttgcgccc gttcctgtgg gttaaaatcg ccctctttac ccccgtgctg cagaattcgc
    16021 cgcagggact catcgcggct gatccctttt aggaattcgt taaactgcgc atcaatgctg
    16081 atgccaattt cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg
    16141 attactccat ccagatcgaa aattacccct tgcagtttca ttccccctcc tgatgcgtgg
    16201 tagcggtccc attgaagggc aaaataaaat tccccaaaca gaaaacagat tcttcagcga
    16261 cgtaaataag ctgaccgttc aagcgcagtg aaacgggcgc agaagttcga atcgcaatac
    16321 gctgcacgtc gagagtgacc tgtaattcgc agccctgcca gaacagaggg gaagacaact
    16381 gttgccactg ctcaggtaac gccggattga gatgtaattc accgtcacgc acgctcaccc
    16441 cggcaaaacc ctgaatcgcc cccagccaga tagcgccagt tgcggcagcg tggataccgt
    16501 catcacagct atgtggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat
    16561 aactttgggt cagcaggcca cagcgtgcgg caacaatgcc gtggattgct ttacttaatg
    16621 acgagtcgtg aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgctg
    16681 agaactgctc cggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca
    16741 tctcgttcac ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt
    16801 tgtatttcgc cagattaatc accggcttag ccataaacga atcatcctgc ggcaaaacgc
    16861 cgtcgggctg aatttctggc agcagcagtt ctttgaggta catttctgca cgatgaataa
    16921 acgcgtcgtc gctacagcca aactgacgag cgatattcag cgcctgctgg acgttgtaat
    16981 acgccatata gctggtgaag gcgttattat tgacatgttc ggtatattca tctggcccaa
    17041 taacatcatg aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg
    17101 ccgtctccag aagtagcgcc atgctttcat gagcaatgaa actttcatcc cccgtggtcc
    17161 gccagtattg aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgcag
    17221 aggccacttt ttgccgcaac ccggtacgaa tgttaatggc agcaaattcc ggcgtctctt
    17281 cttcgccgct acgcgcgctt tcccacggaa acagagcgcc ctgccagccg ttgcgtcgcg
    17341 ctttctcctg cgcgccaagc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg
    17401 tcggatcgct aaacagatgg aacggtagca aaaatacttc tgtatcccag aaaacgtggc
    17461 ctttgtagcc ttcgccggtt aaacctttcg ccgcaatgct gctgcgctca tcgtgggcag
    17521 gcgtcatgat gcgcaggtga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc
    17581 cgccgtttac cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg
    17641 caagaagttg gtcgtaactc tgctgcgcgc acatttcaag ctgacgaagc gatgcactac
    17701 cccactcgtc taaagcgacg tgtctgtcgt cccgccagtc gatccacacc cgtttttgca
    17761 gcgtcagcgt tttgcccgca ggtagttgtg cactggtatg ttgcagcaag cggcgctctt
    17821 tggcggtata gcattgctgc acgtcaccgc tcacctggca gcaacagctg atggccacat
    17881 cactgcgtcc atcctgagtg gtatagatcc cctgcatcag atgctgacca aatacccgca
    17941 cctgggtttc gtcgagatgt tgtcgtccgt ggttggtttg cgtggcgtca atgcctgttg
    18001 aaatcagcac tgacgcgtca gcgtccagcg gcgtaatgct gatttccagc gccatcagcg
    18061 gcagttgttc tgccgaaaca aagcgacggc tggcaatggc ataacgtgcg ccgctggctg
    18121 aagaccagac gacattgcga cgtaattcgc cactggcaaa gttaagctca cgctgccatg
    18181 cttcgcggga taacgagata atctcaccat ttatggcaat ctccatcccc acgacatcag
    18241 gcaggttcac cagttcgttg atttcaccct tacccgcccg atgatacagc cccgtcagat
    18301 acatgccgcg cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc
    18361 cgttcccctg cgccatcagt gaagcatatt tgttcagtgt atgctggctg aaatggggtt
    18421 ctgataacgt tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata
    18481 caacgccgcc accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg
    18541 cttgccctgt acgtgattaa taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc
    18601 catttcccgt tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg
    18661 aaacagcgtc gcaccggctt tatcaccaca aaagctgacg ttcataatcg actgttcgcg
    18721 gatgttgagt gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt
    18781 gccaaacagc gaatcttcga cgctataagt tgccggatcc cactcgccaa attgaccaca
    18841 gctcttttgc gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa
    18901 gcccagcaca tacatcgccg catccagcat atgaatgccg atgtcgatca gcgggccacc
    18961 accctgcagt tctttattgg taaagacact ccagccggga acgccgcagc gacgcagggc
    19021 gcgggcggtg gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg
    19081 ttgcgtatcg agcgcaaaac gatggtgaaa gtcgtaggcc agcaccttgc ccagtttgcg
    19141 cgcggtatcg cacatttccc gcgcctgttc tggcgtcatg gcgggcggtt tttcgcacat
    19201 cacatggcag cccgcttcca gtgccatcag ggtatgttcg taatgaaaac ggttaggtga
    19261 gcagacgcta accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac
    19321 ggatgcattc ccgtattttt ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac
    19381 agccaccagt tccagatcgt tgcgggtgca gtagtacgaa gcatgaactt tatccgccac
    19441 ctggcccgcg cctatgatcg cgactcgcag cggagagctt gtcattgcac ttttcacaaa
    19501 gacctcttag caggtacgca cccaggccaa cgaatcacgg tacgcctggg caggatcttc
    19561 tgcccggata cgcccttcat acaccacata accctgatag ttatcggcgc gcagctgttc
    19621 aaacagcgcg tggaaatcga gggtgccgct gcccggctgg taacgatggt tgtcggcaat
    19681 atgcacatga ccgagcaagt cgcggttgtc gtgcaacgcc tgcgccaggt tatcttcttc
    19741 gatattcatg tgatagaaat cgccgataat ctgcacatgt ttaagattgt tttcgacgat
    19801 gtaacgacga gcatcggcga gggtgttgat catgtgatcc tgatagcggt ttaacggttc
    19861 gaggtacacc acggttccgg tacgtgcggc cgcctgttcc agtacgcgca gggaatcact
    19921 caccattttg cggtcgccgt ccaggctacg cggcgaggtc atcggcggta agcggaaggt
    19981 aaacatgccc catgcagccg gaacgacgac acctttaccg cccacttctg ccagagcttc
    20041 aagaatgcgt tcgatctgct ttacgccatt aagtcgacgc tcttcgataa agtcgccaat
    20101 ccaaccgtca tagccaccgc aggcggtggt caccggtaaa ccggtttctt tgattgctgc
    20161 tttgacttct tcgagattgt tgaccagcag tttgccgtcg atctcaaaac catcgaaacc
    20221 catttctttg atataacgaa atttctccag aatgttttcc ggaaagaacg cctgattctg
    20281 tgtgccgatt ttcatttttt tcattccttt gaattaaaac gtaacgccca ttttgatgct
    20341 ctgttccgga tgctggtcga catactgcat gtaactttcc gggctattgg caaaggtcac
    20401 caccgggtcg attaaatctt cacaattgag ataaccgttc atcagcagtt cccaacaggt
    20461 ttcttcgata cgtttgcggc tccagcgcgg ataatccggg ttcggctcgc tgcacgcacg
    20521 ggagaaaaca attttggcgt tattgaaatg ggcttcgcgt ccgaggttaa aaccttcggc
    20581 aaacggtttg gcaaacgcga catacgagat ggtgccgcca tatgccaggc cgcgaagcgc
    20641 cgattgcagc gcgtcggcgt aaccgctggt ttcgataatc acatcggcac cctgcttgcc
    20701 ggtcagcgtt ttgatctctt tgcctacgtc agtgccaata gggttaaggc agaaatccgc
    20761 accgtgtcga cgagcaatat cacaacgatg ggcaatagga tcgacgccaa ttaccaccga
    20821 tgcgccagcg cgtttcgcca gttggatggc aatttgaccg atcgcgccaa gccctaccac
    20881 cacgacaaaa tcccctacgc gtacgttggc atcgcgcacg ccgctcatgg caaactgcgc
    20941 cgggtcgtaa cagacggcgt ttttccagga gctgccttgc ggcattttgc gcagcttgta
    21001 gttattcact gcgttaatga tgaccgtctc ggagagcggg ccgtagccgc atacgctgtc
    21061 gcccaccgcg tagtcggtaa cgtcgctgcc gcactcgata atgtcgccaa ccaccatgtt
    21121 gccaagctgg aatttgccaa actcaatacc gcgcggcgca tctgccggac gcggggtaaa
    21181 catctgccat tcgccgttaa agtcttcatc gataaacggg ctggcggcgc ggaaatcgac
    21241 cacttccgtt ccatgtttcg gcgcgccgaa acgcacgcgg atcttcactt cactggctaa
    21301 aatcgcccga tcttcatact caaccagcgc agctacgcgc ggtgctgtgg ctactaactt
    21361 tttcatgtaa aactccttat aaagaaactg gccgctttag cccttcacgc caccggcggt
    21421 caaaccactc ttaataaaac gctccgaaag cgcatacata atcaccaccg gcaatgcggt
    21481 caccagcgag gccgccatca tccgtcccca gatgtaatca ggcgtactga acagcgcgtt
    21541 caggccgact ggtaaggtga aattgctggc gctggagagg aaaatcgacg caaacagata
    21601 gtcgttccat gccaccataa agcagtagac gaatacggat atcagcccgg acatcgccag
    21661 gggcacggtg atacggaaga tgatttgcag acggttgagg ccatccatca tcgccgcttc
    21721 ttcgatctca tcagggatgg tgtcgaagta gcttttcagc ataaacacgg cggtgggcag
    21781 ggtttgcgtt accatggtga tgatcagcgc catctcggtg tcgtatatgc caagcgcggt
    21841 gatgattttg aacagcggca ccaccagcaa aatgccggag aacatataca ccgtgtaaaa
    21901 gctggcgttg atggtcatcc gccctttaaa gcgcaggcgg gaaagcgcgt aagccccgag
    21961 aatgccgaga aataccgcca ccacggatga aaccacagac accaccagac tgttacggaa
    22021 gtagtcgaca aacgggaaaa tcatcgggtt aaaaatgtcg acgtaatgct ccagcgtcca
    22081 ctgctgtggc agcagcgtag gatgcagcga gatcgcctct tttgcgctct tgaacgaggt
    22141 catcagcatc acaaaaaacg gaaacagcat gatgatgaga aacagcgcca gcccgcagta
    22201 aaaaccgatg cgactgagtg tgcgtttatt tgttgccatt gaggttcacc cgtttcctgg
    22261 tcagcagaat gacagcgaaa ataatgatga agagcaccac cgagatcgcc gccgctttcc
    22321 ctaggtcatt aaaggcaaag gcggttttgt agagatagac accgagaata tcgaccttgg
    22381 tcgtcagcag ataaacatcc gcgaacatgt agaacatcca gatggtgcgc agggtcacca
    22441 ccgtcgccag gaccggcata attgcgggca gcgtgacgat acgaaaccgt tgccaggcat
    22501 tagcgccatc catctctgcc gcttcgtaca gcgatttgtc gatggtctgc aaaatcgcca
    22561 gaaacgaaat aaaggcatag gggaagtagc gccagatggc gaacagcacc accagcacaa
    22621 agctactgcc cggattatcg aaccacagcg gtgcctgctc atagagatgc agaagatcga
    22681 cgccgaggta gttcacaatg ccgtaaccgt tgttgaacat gtatttccag gcgaacacca
    22741 gcgaaataga cggcgttacg taggagagga gcaccagcga acgcgcggtt ttgcgcaggc
    22801 ggaactcgcg gttgaaaaac atcgccacgg ccagcccgag cacggtgctg cccgccacca
    22861 ccagtgtggt ataccagacc gtcatccaca gcgagtgcca gaagccggga tcggagagaa
    22921 tacgcacgta gttgcttacc ccaacaaacg ttgactcgat gttgggattg agcggcagac
    22981 gtaaaaaact gatttcgata ttcgacacca ttggccacgc caccagaccg cccagcagta
    23041 ataagctggg cgcgagaagc agcagcgcaa agggcatatc ggaacgacct gaaaaaagcc
    23101 tgttcattgc ggattcctgt cctgggttta gcgctgttca accaactcat ccagcttttt
    23161 ctggctcgtt tgcagcgtgg tagagaggtc ggctttaccc acggtgacgt tatgcaccat
    23221 ggagctcacc acgccagaac ccgtcacatc gcccatgcgg gtaaaatttt tatcccctac
    23281 tgcgccaaag acctgaatat ttggcagttc gctgattaac tgatttggca attctcccag
    23341 cgccttaata acgtcgttgt ctttccaggt ggcggtagtg acaaccgctt tgttcaccgg
    23401 cagcgccgcg cctggcgaca tcatcaccca gtcggcaatg ttgtctgcct gctccataaa
    23461 ggtgacaaat ttttcagccg cttcggtctc ttcgctcttt tgcccggcgg taatggtcag
    23521 cgaggtcaac atgccgtaga ccgcagagtt tttctcggtg ggtacaacga aaccaacgtt
    23581 tttcggatcg ccctctttaa tcacagccgg aaggatatag gtggagtaaa tcgccatcgg
    23641 cgcggtgccg ttcataaaag cgtctttcac ttccatgatg tcgttagaac ctggcatggt
    23701 gttggcagca aggttgcggt aataggtcag cgcctgcatc atctctggtg tatcaagggt
    23761 gattttgcct tcggcgttaa agacgttagc ctgattggat aacgcaaact gggagaaaga
    23821 ctgttccgtc aacacgcttt ctgctgtagg cagcgcaata ccgtattttt tattcgccgg
    23881 gtcattcagt ttctgtgcaa cgtccagcaa ttgctgccag ttttttggct cctccagccc
    23941 agcttttgcc agcacatctt tgcgatacca gatcccgcca atccatgcgc tgacaggaac
    24001 accggtccag gcgctgccat cttcggtgcg cacaatccgc agtacgccat cgtagaacgc
    24061 gccttcaccg acgttgctga tgaccgtggc aaccgcctgg cgatcgataa gctgctcttt
    24121 gtccatcact ttggcgtagt catggctggt ttcgatcact tccggcagcg aaccgctacg
    24181 tgaaagggta atgactttgg tgttataggc gtcttcttcc acgggcactt gttttacggt
    24241 gatacctggg ttttcttttt caaaacgggc aatcaatttg ctgatgacgg cctggcgctc
    24301 ctgctccacc gaagaatgca taaattcgat ggatacatta tttttatttt cttctttaca
    24361 gcctgaaatc agggcgcagg aaaccagtgc tgataacagc acgatttttg atttaatcat
    24421 gagatgttcc tttttattta atccacataa cctgccaggg gcgcagcgtt atttcattac
    24481 ccactatatc aacttcacta at
//
LOCUS       DAZFRG010000050        24456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFRG010000050 DAZFRG010000000
VERSION     DAZFRG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.050"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..803
                     /locus_tag="ACHKUC_001582"
     CDS             <1..803
                     /locus_tag="ACHKUC_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6617858.1"
                     /translation="IAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWGIPFVL
                     LVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMIKDDAF
                     LALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGINVTRL
                     KIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGYGTVVG
                     TVIGALTIGVINNGMNLMNVPYFYRMVVKGLVILVAVYFDVRNKRKRS"
     gene            825..1787
                     /locus_tag="ACHKUC_001583"
     CDS             825..1787
                     /locus_tag="ACHKUC_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6617859.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKENGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIETFIQRHDMSIREAAQ"
     gene            1784..2830
                     /locus_tag="ACHKUC_001584"
     CDS             1784..2830
                     /locus_tag="ACHKUC_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO6617860.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDNIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTL
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(2922..4271)
                     /gene="accC"
                     /locus_tag="ACHKUC_001585"
     CDS             complement(2922..4271)
                     /gene="accC"
                     /locus_tag="ACHKUC_001585"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO6617861.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4282..4752)
                     /gene="accB"
                     /locus_tag="ACHKUC_001586"
     CDS             complement(4282..4752)
                     /gene="accB"
                     /locus_tag="ACHKUC_001586"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO6617862.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5157..5544)
                     /locus_tag="ACHKUC_001587"
                     /pseudo
     CDS             complement(5157..5544)
                     /locus_tag="ACHKUC_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            complement(5730..6704)
                     /gene="yhdH"
                     /locus_tag="ACHKUC_001588"
     CDS             complement(5730..6704)
                     /gene="yhdH"
                     /locus_tag="ACHKUC_001588"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO6617863.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            6856..8796
                     /gene="csrD"
                     /locus_tag="ACHKUC_001589"
     CDS             6856..8796
                     /gene="csrD"
                     /locus_tag="ACHKUC_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO6617864.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTYLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9101..10144
                     /gene="mreB"
                     /locus_tag="ACHKUC_001590"
     CDS             9101..10144
                     /gene="mreB"
                     /locus_tag="ACHKUC_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO6617865.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10210..11310
                     /gene="mreC"
                     /locus_tag="ACHKUC_001591"
     CDS             10210..11310
                     /gene="mreC"
                     /locus_tag="ACHKUC_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO6617866.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGG"
     gene            11313..11801
                     /gene="mreD"
                     /locus_tag="ACHKUC_001592"
     CDS             11313..11801
                     /gene="mreD"
                     /locus_tag="ACHKUC_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO6617867.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11810..12403
                     /gene="yhdE"
                     /locus_tag="ACHKUC_001593"
     CDS             11810..12403
                     /gene="yhdE"
                     /locus_tag="ACHKUC_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO6617868.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTEQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12393..13862
                     /gene="rng"
                     /locus_tag="ACHKUC_001594"
     CDS             12393..13862
                     /gene="rng"
                     /locus_tag="ACHKUC_001594"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO6617869.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13930..17730
                     /gene="yhdP"
                     /locus_tag="ACHKUC_001595"
     CDS             13930..17730
                     /gene="yhdP"
                     /locus_tag="ACHKUC_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO6617870.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLLAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELKLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKIGFLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPVRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17886..19331
                     /gene="tldD"
                     /locus_tag="ACHKUC_001596"
     CDS             17886..19331
                     /gene="tldD"
                     /locus_tag="ACHKUC_001596"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO6617871.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19459..20388)
                     /gene="aaeR"
                     /locus_tag="ACHKUC_001597"
     CDS             complement(19459..20388)
                     /gene="aaeR"
                     /locus_tag="ACHKUC_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO6617872.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20571..20774
                     /gene="aaeX"
                     /locus_tag="ACHKUC_001598"
     CDS             20571..20774
                     /gene="aaeX"
                     /locus_tag="ACHKUC_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO6617873.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20782..21714
                     /gene="aaeA"
                     /locus_tag="ACHKUC_001599"
     CDS             20782..21714
                     /gene="aaeA"
                     /locus_tag="ACHKUC_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO6617874.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21720..23687
                     /gene="aaeB"
                     /locus_tag="ACHKUC_001600"
     CDS             21720..23687
                     /gene="aaeB"
                     /locus_tag="ACHKUC_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO6617875.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            23779..24051
                     /gene="yhcO"
                     /locus_tag="ACHKUC_001601"
     CDS             23779..24051
                     /gene="yhcO"
                     /locus_tag="ACHKUC_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO6617876.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24106..24369)
                     /gene="yhcN"
                     /locus_tag="ACHKUC_001602"
     CDS             complement(24106..24369)
                     /gene="yhcN"
                     /locus_tag="ACHKUC_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO6617877.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5796 a         5935 c         6648 g         6077 t
ORIGIN      
        1 taattgcaat taccgcctat ggaatgactt atgtgctttt gcttggcgat attgatttat
       61 cggtcggttc aaccattgca ttgattggca catttgctgc gcttggggct tcatggggaa
      121 ttccatttgt tttattggtc ccattatcca ttattgcagc actggctctg gggatgataa
      181 atggtggctt gacggctata gcggggatcc cttcgtttat tgtcaccgtt gctactatgg
      241 ggatcttccg tggtatcgct tatatagtga ccgatggtat gcccatcatg attaaagatg
      301 atgcttttct ggcgctgggg aatggagaat ttctctatat acctattcct atatggatat
      361 taatcatctt attgctgata aaccacttca ttcttactaa aacaaccttt ggacgaaaaa
      421 tttatatcac tggtggtaat aaagaagcag caatttattc agggataaat gtcacccgct
      481 tgaaaattaa agtctttatg attacagccg tgctggcagg tatcagcggg atgatccttg
      541 cctcacgcct ctattccggg caaccgaatt ccgcgttgag ctacgaactg gatgctattg
      601 ccgcagctgt actgggaggc accagcctga acggcggcta tggaaccgtg gtggggacgg
      661 tgatcggcgc attaaccatc ggcgttatta ataatggcat gaacttaatg aatgttcctt
      721 atttttaccg gatggtggtt aaaggattag tcattcttgt agccgtttat tttgacgtaa
      781 gaaataaaag aaaacgcagc taatcatttc aaggaattaa tcatatgagt aaggtcttca
      841 caattggaga aattctggtt gaaattatgg cgagtaaaat cggccagcca tttgatcaac
      901 caggtatctg gaacggccct tatcccagtg gtgcgcccgc tatttttatc gaccaggtga
      961 cacgtcttgg cgttccgtgc ggcatcatta gttgtgtggg gaatgatggc tttggtgata
     1021 ttaatataca tcgtctggct gctgacggtg tggatatcag ggggatttca gtcttacctc
     1081 tggaagctac cgggagtgcc tttgttactt atcataactc tggcgatcgt gattttattt
     1141 ttaatatcaa gaatgccgct tgcggtaaat tatcggcgca gcatgttgat gaaaatattc
     1201 tgaaggactg tacccatttt catattatgg gttcatccct gttttcattc catatggtag
     1261 acgcagtaaa gaaagcggta accattgtaa aagagaatgg tggcgttatt tcttttgacc
     1321 ctaatattcg gaaagagatg ctcgatattc cagaaatgcg tgacgccctc cactttgttc
     1381 ttgagttaac ggatatctat atgcccagtg agggagaagt gttgctcttg tcgccacact
     1441 cgacgccgga gcgtgcaatc gctggttttc ttgaagaagg tgttaaagag gtgattgtga
     1501 agcgcggcaa tcaaggagcc agttactact ccgcgaatga gcagtttcat gttgagagct
     1561 atccggtgga agaagttgac ccgaccggtg cgggagactg ttttggtggc gcatggattg
     1621 cctgccgcca gctagggttt gacgcccatc gggcattaca atatgccaac gcctgcggtg
     1681 cactggcggt aacccgacgt ggcccaatgg agggaacgtc tcgccttgcg gaaatcgaaa
     1741 cgttcatcca gcgccatgac atgtccattc gggaagcagc acaatgaaaa acgaaatttg
     1801 gtcgtggatt ggcgatctct cgcaggttgc cggaattaaa cactatgagt tacgtagtgg
     1861 cagagcaaag ggtacagaag cgtttgatgt ccgtactggt gcgggactgg catttacggt
     1921 ggtaaaagat cgcgcgctgg atattgcatg ggccagctac aaggacactg cgctctcttt
     1981 tatcaccccc aacggtgttg tcgcccccgc tttttttgag tctcagggca acgggttttt
     2041 acgtagcttt tacgcggggt tactgactac ctgcggactt agctacattg gcacgccctg
     2101 tgaggatgaa ggagaaacgc tggggttaca cgggcgtttg gctgcgactc cagcagaaga
     2161 ggtggggtat cgcacagaac ggaccgacga caacattgaa tttgtcatca atggcaaagt
     2221 acgggaaaca cgattgttcg gtgaaaatct gacgctggaa aggaccattc gctgccgtta
     2281 tggcgaaaat gtaatgcgca ttgaagacaa ggtgactaat catggtttca cccgccagcc
     2341 gctgcaaatt ctttatcact ttaattacgg ctggccttta ttatcgccac aggcagaaat
     2401 cttgctgtcg gctaaaagca taacgccacg aactctgcat gcggcggaag ggttaaccag
     2461 tcatctggag atttgtaccc cacagcccgg ttttgacgag caggtttact atctcacgtt
     2521 gaatagcgac agtcagggga tgagtaaagt tgcgctggtg aacgccgaac tggggtgggg
     2581 aatttacgag aaattcgaca ccatgcagct acccaacttt atccagtgga aaaatttagg
     2641 cgcgggtgaa tatgttatgg gactggaggt cagtaatagt tttcctgacg gtcgtgataa
     2701 agaacgagcg caggggcgtc tgccctttat tgagccaggg gaaacaaaga aatactgctt
     2761 tgagctggga attgttgatg gcgatgctga aattagcgcg ttgaaagcgg aaattgcagg
     2821 ctaccgttag ttaacggcaa taaaaacgcc gggtagcatt aacaggcccg gcgttgatta
     2881 cgttctttgc taagcgcgat gaaaacaccg cgcgtcacaa attatttttc ctgaagaccg
     2941 agttttttct ccagatagtg gatgttagtg ccaccatgct ggaagttctc gtcattcatg
     3001 atgcggatct gcagatcaac gttggttttg ataccgtcga tgatcagctc ctgcagcgca
     3061 ttcttcatgc gagcaatcgc cacgtcacgg ttttcaccgt agcaaatcag cttaccgatc
     3121 attgagtcat agtacggcgg tacggtgtag cccgcgtaga tatgagactc ccaacgtacg
     3181 ccaaaaccac caggtgcgtg gaaacgggtg attttgcccg gacttggcag gaaggtgttc
     3241 ggatcttcgg cgttgatacg gcattccacc gcatggccgc gaacgtgaac ttcttcttgc
     3301 ttgatcgaca gcggttgacc ggcagcgata cgcagctgtt ctttgatcag gtcaacgccg
     3361 gtgatcattt cagtaaccgg gtgttctacc tgaatacggg tgttcatttc gatgaaatag
     3421 aactcgccgt tttcgaacag gaactcgaaa gtacctgcac cgcgatagcc gatatcaaca
     3481 cacgctttag cgcaacgttc gccgatgtag cgacgcagtt ccggggtaat gcccggagcc
     3541 ggtgcttctt cgaccacttt ctggtggcgg cgctgcatgg agcagtcacg ttccgccaga
     3601 tagatagcgt tgccctgacc gtcagccagt acctgaatct cgacgtggcg aggattttcc
     3661 aggtatttct ccatgtaaac catatcgttg ctgaaagcag ctttcgcttc cgcacgggtc
     3721 atggagatgg attgtgccag ttcagcgtcg ccgcgcacta cgcgcatacc gcgaccgccg
     3781 ccgccgccgg aggctttgat aattaccgga taaccaatgc gtttagcaat ggcgcggttt
     3841 ttatccatat cgtcgcccag cgggccgtca gaacccggta cgcaaggaac gcctgctttt
     3901 ttcatcgccg cgattgcgga tactttgtcg cccatcaggc gaatggtttc tgctttcggg
     3961 ccgatgaaga taaagccgga gcgttcaacc tgctcggcaa aattggcgtt ctcggagagg
     4021 aagccgtaac ccggatggat tgctactgcg ccggtgattt cagcggcgct gatgattgcc
     4081 gggatgttca gataactttt tactgacgga gcagggccga tacagaccgt ttcatctgcc
     4141 agtaatacgt gttttagatc gcgatccgca ctggagtgca cagcgacagt cttgatgccc
     4201 agttctttac aggcacgaag aatacgcaat gcaatctcgc cgcggttggc aataacaatt
     4261 ttatccagca tgttcgcctc gttactcgat gacgaccagc ggctcgtcaa attctaccgg
     4321 ttgtccactt tcgaccagaa ttgctttcac ggtaccggat ttgtccgctt cgatctggtt
     4381 catcattttc atggcttcaa cgatgcacag ggtatcgccc acgttgactt tctgacccac
     4441 ttcgatgaac gcttttgcgt ccgggcttgg ggtgcggtag aaagtaccaa ccatcgggga
     4501 acgtacgatg tgaccactga tttccgctgc tgctggcgct tccatggaag gaacggtcgc
     4561 cggagcggct gcgttagatt gagctggttg ctgcatcata ggtgcagcgt aagcttgttg
     4621 catcacaggg aaacttgcgg caggagctgc acggctaatg cgtactgact cttcgccttc
     4681 agaaatttcc agttcggaga tgcctgattc ttcaaccagc tcgatcagtt ttttaatctt
     4741 acgaatatcc atgagtgggt tccgtactct ttgtttagtg tgattgtgac aggcgtttca
     4801 ccgccgtctg taaagcgtat gccccgacag ctgtatgcat agcgataaat tccagcaggc
     4861 cgggacgccg gtctattttg ccccagagtg tcaatgttag acttgacgga cattgtgcag
     4921 cgatctgctg ctatcctccg gcaaaaaaca aaatatacct tcgttgcgcc aatgtcacct
     4981 tttccgcagc gcaaaaactg cgtcagggag gacgaggtcg cacattataa cgatttcgta
     5041 gcaattggca gctaaatact ggtcttatca gggaagataa tcaacagcta acatgtaaat
     5101 aaccttcaac accgtgtaat ttgcaacaag ccgcacaatt cacgaaatta gcgccatcat
     5161 cgacggaact tcttataacg taaggtgaaa agctgtaaag ccagccctgc gaagatgggg
     5221 ggctgcaatg agataatctt cacagaccac aaataatgta gggatgccag gatcgcgaca
     5281 agatagacga cattacgcag cggttgctgg ggattgccca attttcactg catcgtccgg
     5341 aaagatgtga atgctaaagc acgcaaaatt aaccgactga caatgttccg cattcaatag
     5401 gtttaagtgg ttaatttgca atcaaataaa atcagattat tccccccatg gtaccagaaa
     5461 tcagtaacag attaagcgta gcgtcgccaa ggcaaaacac cataaaccta acaggcgacg
     5521 gaggcgtatc aataatggct gtttggtgga gcatcaatgc gcatcaattt atggtcagtc
     5581 gaaatcatga ctttccagtt tggatgatgt tgtacaactt catgaccaag atgttcgacg
     5641 aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga ataatgattt caaaatgagt
     5701 tccctctctt attattccct gctaaatggt tagttaacct tcaccagcgt gcgaccctgg
     5761 atctggttat taatgatggc ctcggcaaac ttcggtgcct ctgccagaga tatctctttt
     5821 gccgcctggg tatagaatga ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg
     5881 cggcgtgctg gtggcgtcat tactgaatcc accccttgca aacggacatt acgcagaata
     5941 aatggcatga ccgtggttgg cagagtaaaa ccacccgcca gaccacaggc cgccacgcag
     6001 ccgccgtaat tcatttgcgc cagcactttt gccagcactt tgtcgccaac ggtgtcaatt
     6061 gccccagccc agacttgttt ttccagagga cgggattcag caaactcatc gcgaggaaga
     6121 atacggctgg cacctaaact tttcagatat tcatgggtac tttcgcgacc ggaaacagca
     6181 acgacctgat aacccaactt atgcagcagc gccacggcgg tactgccgac gccaccactg
     6241 gcacccgtca cgacaatctc cccatcctgc gggcgaacac cggcatcttc cagcgccatc
     6301 acacacagca tggcggtaaa accggcagta ccgataatca ttgctttacg cgcgtccagc
     6361 ccttgcggca tggcaaccag ccagtcacct ttcactcgcg cctgttccgc cagcccaccc
     6421 cagtggtttt caccaacgcc ccagccagtg agtaacacct cctgaccggc atgaaaacgc
     6481 ggatcttcgc tggtgcgtac agttccggca aaatcgatcc caggaatcat cggaaaatta
     6541 cggatgattt ttcccttacc ggtaatcgcc agcgcatctt tgtagttcag gctcgaccag
     6601 tgaacatcaa ccgtgacatc gccttccggc aggcgacttt cgtccagagt ctgtactgat
     6661 gcgagagttt tgccgtcctg ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg
     6721 catgatggat tggaaaataa tttctgaaga ctatactcgc taatggaaaa gcaaagccga
     6781 tgaagcgcaa taaattgcga gataaatctg atttgctagt atgcccgctt cctcactatc
     6841 ggagttaaca caaggatgag attaacgacg aaattttcgg cctttgtcac gctgctcacc
     6901 gggttaacaa tttttgtgac tttgctgggc tgttcgctaa gtttctacaa cgccattcag
     6961 tataagttta gtcatcgtgt tcaggcggtg gcgacggcga ttgataccta ccttgtgtcg
     7021 aatgacttca gcacattaag gccacaaatt accgaattaa tgatgtcggc agatatcgtt
     7081 cgtgtagacc tgctccatgg tgataagcag gtttataccc tggccagaaa tggtagttat
     7141 cgtccggttg gcaccaacga tctgtttcgt gaactgagcg ttccgttgat aaagcatccg
     7201 gggatgtcgc tgcgtctggt ttatcaggat ccgatgggca actatttcca ttctttaatg
     7261 actaccgcgc cgctcacggg ggcgattggc tttatcattc ttatgctctt cctggcggta
     7321 cgctggttac aacggcaact tgccgggcaa gaattgctgg aaacccgggc tactcgtatc
     7381 ttaaacggtg agcgtggctc taatgtgttg ggaaccatct atgaatggcc gcccagaacc
     7441 agcagtgcgc tggatacgct gctttgtgaa attcagaacg cacgcgaaca acacagccgt
     7501 cttgatacgc tgatccgctc ttatgccgcc caggacatga aaaccggcct caataaccga
     7561 ctctttttcg ataatcagtt agcaacgtta ctggaagatc aagagaaagt aggtacccac
     7621 gggatcgtga tgatgattcg tctgccggat ttcaatatgt tgagtgatac ctgggggcac
     7681 agccaggttg aagaacagtt cttctctctg acgaatctgc tatcgacatt tatgatgcgc
     7741 taccctggcg cactgctggc gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac
     7801 cggacgttaa aagaggcaga gagcatcgcc agtcagttaa tcaaagccgt cgataccttg
     7861 ccgaacaata aaatgctcga tcgcgacgat atgatccaca ttggtatctg tgcctggcgt
     7921 agtggtcagg ataccgagca ggtaatggaa catgcagagt ctgccacgcg taatgcggga
     7981 ttgcagggcg gcaatagctg ggctatttac gatgactcgt tgcctgaaaa aggacgcggt
     8041 aatgttcgct ggcgtacgct tatcgagcaa atgctcagtc gcggcggccc gcgcctttat
     8101 caaaaaccgg cggttactcg cgaaggccgg gttcatcatc gtgaactcat gtgccgcatc
     8161 ttcgatggta atgaagaggt tagctcggca gagtatatgc cgatggtctt gcagtttggt
     8221 ttatcggaag agtatgaccg cctgcaaatc agccgtctta ttccactatt gcgttactgg
     8281 ccagaggaaa atctggcgat tcaggttacc gttgagtcgc tgattcgccc gcgttttcag
     8341 cgttggctgc gcgatacgtt aatgcaatgt gaaaaatcgc aacgaagacg cataattatt
     8401 gaacttgcag aggccgatgt tggtcaacat atcagtcgct tacaacctgt tattcgttta
     8461 gtgaatgctt taggggtacg ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc
     8521 agttggatca aagaacttaa tgttgagtta ctcaagctcc atccggggct ggtcagaaac
     8581 attgagaagc gaacggagaa ccagctgctg gttcaaagcc tggtggaagc ctgctccggg
     8641 accagcaccc aggtttacgc caccggcgta cgttcgcgaa gcgagtggca gaccctgatt
     8701 cagcgcggtg ttacaggtgg gcaaggggat tttttcgcgt cctcacagcc acttgatact
     8761 aacgtgaaaa aatattcaca aagatactcg gtttaacctg ccgtttaatc cgttttcacg
     8821 tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc tgctcgccag attgttgcag
     8881 cacatatgca gatgaatgac cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca
     8941 ttaagccttt ctggactcag gcagagattt gtaacaaagg aaacgaactg cactaatttt
     9001 caccgtagca gatgattttt gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg
     9061 attttctttt ccgccccagc tttcaggatt atcccttagt atgttgaaaa aatttcgtgg
     9121 catgttttcc aatgacttgt ccattgacct gggtactgcg aataccctca tttatgtaaa
     9181 aggacaaggc atcgtattga atgagccttc cgtggtggcc attcgtcagg atcgtgccgg
     9241 ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc
     9301 gggcaatatt gctgccattc gcccaatgaa agacggcgtt atcgccgact tcttcgtgac
     9361 tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc aacagcttta tgcgtccaag
     9421 cccgcgcgtt ctggtttgtg tgccggttgg cgcgacccag gttgaacgcc gcgcaattcg
     9481 tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg attgaagaac cgatggctgc
     9541 cgcaattggt gctggcctgc cggtttctga agcgaccggt tctatggtgg ttgatatcgg
     9601 tggtggtacc actgaagttg ctgttatctc cttgaacggc gtggtttact cctcttccgt
     9661 gcgcattggt ggtgaccgtt tcgacgaagc tatcatcaac tatgtacgtc gtaattacgg
     9721 ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac gaaatcggtt cggcttaccc
     9781 gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac cttgcagaag gtgttccacg
     9841 cggttttacc ctgaactcca acgaaatcct cgaagcactg caggaaccgc tgaccggtat
     9901 tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg gaactggctt ccgatatctc
     9961 cgagcgcggc atggtgctca ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt
    10021 aatggaagaa accggcattc cagtcgttgt tgctgaagac ccgctgacct gtgtggcgcg
    10081 cggtggcggc aaagcgctgg aaatgatcga catgcacggc ggcgacctgt tcagcgaaga
    10141 gtaatcggat gcaggcaggg gaagtgtctg tttaccctgc ctggtctgat acgagaatac
    10201 gcataactta tgaagccaat ttttagccgt ggcccgtcgc tacagattcg ccttattctg
    10261 gcggtgctgg tggcgctcgg cattattatt gccgacagcc gcctggggac gttcagtcaa
    10321 atccgtactt atatggatac cgccgtcagt cctttctact ttgtttccaa tgctcctcgt
    10381 gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg accaactaga acttgaaaac
    10441 cgggcgttac gtcaggaact gttgctgaaa aacagtgaac tgctgatgct tggacaatac
    10501 aaacaggaga acgcgcgtct gcgcgagcta ctgggttccc cgctgcgtca ggatgagcag
    10561 aaaatggtga ctcaggttat ctccacggtt aacgatcctt atagcgatca agttgttatc
    10621 gataaaggta gcgttaatgg cgtttatgaa ggccagccgg ttatcagcga caaaggtgtt
    10681 gttggtcagg tggtggccgt cgctaaactg accagccgcg tgctgctgat ttgtgatgcg
    10741 acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac
    10801 ggttgtacgg atgatttgca gcttgagcat ctgccagcga atacggatat tcgtgttggt
    10861 gatgtgctgg tgacttccgg tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt
    10921 gtctcttccg taaaactcga tacccagcgc gcttatactg tgattcaggc gcgtccgact
    10981 gcaggactgc aacgtttgcg ttatctgctg ctgctgtggg gcgcggatcg taacggcgct
    11041 aacccgatga cgccggaaga ggtgcatcgt gttgctaatg aacgtttgat gcagatgatg
    11101 ccgcaggtat tgccttcgcc agacgcgatg gggccaaagt tacctgaacc ggcaacgggg
    11161 atcactcagc cgactccgca gcaaccggcg acaggaaatg cagttactgc gcctgctgcg
    11221 cccacacagc ccgcagctaa tcgctctcca caaagggcta cgccgccgca aagtggtgct
    11281 caaccgcctg cgcgtgcgcc gggaggataa tagtggcgag ctatcgtagc cagggacgct
    11341 gggtaatctg gctctctttc ctcattgcgc tgttgctgca aatcatgccc tggccggata
    11401 atctgattgt tttccggcca aactgggtgt tactcatctt gttgtattgg atcctggcct
    11461 tgccccatcg cgtaaatgtg ggcacaggtt ttgtgatggg tgccatactg gatctgatca
    11521 gcggctcgac gcttggcgta cgcgtattgg cgatgagcat cattgcttac ctggtggcgc
    11581 tgaaatacca gcttttccgc aacctcgcat tatggcagca ggcgctggtc gtcatgttgc
    11641 tttcgctggt ggtggatatt attgttttct gggcagagtt tttagtgatt aacgtctctt
    11701 tcagaccgga agtgttctgg agtagtgtag tcaatggggt gctctggccg tggattttct
    11761 tgctgatgcg taaagtccgt cagcagtttg cagtgcaata aaggtttcta tgacttctct
    11821 gtatttagct tccggttctc cgcgtcgtca ggagttactt gcgcaacttg gcgtgacctt
    11881 tgaacgtatt gttacgggca ttgaggagca gcgtcagccg caggagagcg cgcagcagta
    11941 tgttgtgcgt ctggcgcgcg agaaagcaca ggcgggtgtc gcgcaaacgg agcaggatct
    12001 cccggtgctg ggtgcggata ctatcgttat cctgaacgga gaagtgctcg agaaaccgcg
    12061 cgacgcagag catgcggcgc agatgttgcg caaattatcg ggtcagaccc atcaggtgat
    12121 gacggcagtg gcgttggctg acagccagca cattctcgat tgcctggtgg tcaccgatgt
    12181 gactttcaga acgttaacag acgaagacat cgcgggctat gtcgccagcg gcgaaccgtt
    12241 agataaagca ggtgcatacg gtattcaggg gctgggtggc tgttttgtca ggaagataaa
    12301 tggcagcttt cacgccgtag tcggcttacc gctggttgaa acgtatgaat tattaagtaa
    12361 ttttaacgca ctgcgtgaga aaagggataa acatgacggc tgaattgttg gtaaacgtaa
    12421 cgccttcgga aactcgagtg gcgtatattg atggcggtat tctgcaggaa attcatattg
    12481 aacgtgaggc gcgacgcgga atagtaggca atatctacaa gggtcgtgta agtcgtgtac
    12541 ttccgggtat gcaggcggct tttgtagata ttgggctgga taaagccgcg tttcttcatg
    12601 catctgacat catgccgcac actgaatgtg tggcgggtga agaacaaaag caattcacgg
    12661 tgcgcgacat ctcggaactg gtccgtcagg ggcaagatct gatggtgcag gtggtgaaag
    12721 atccgcttgg cactaaaggt gcgcgcctga ccaccgatat cacgctgcct tctcgctatc
    12781 tggtgtttat gccaggggct tctcacgttg gggtatccca acgaattgaa agcgaatcag
    12841 aacgtgaacg cctgaaaaaa gtggtcgcag agtattgcga cgagcagggc gggtttatca
    12901 tccgtactgc agcggaaggg gttggcgagg ctgaactggc ctccgatgcc gcttatctga
    12961 aacgcgtctg gaccaaagtt atggagcgta aaaaacgccc gcagacccgt tatcagctgt
    13021 acggagaact ggcgctggcg cagcgtgttc tgcgtgattt cgccgacgcc gaactcgacc
    13081 gcattcgcgt tgactcacgc ctgacttacg aagcattgct ggagtttacc tcggagtaca
    13141 ttcccgagat gaccagcaag ctggagcatt acaccggacg ccagccgatt ttcgatctct
    13201 ttgatgtcga aaacgaaatt cagcgagcgc tggaacgcaa agtagaactg aaatccggtg
    13261 gttatctgat tatcgaccag accgaagcga tgaccaccgt ggacatcaat accggagcgt
    13321 ttgtcggtca tcgcaatctg gacgacacca ttttcaatac caatattgaa gcgacgcagg
    13381 ctatcgctcg ccagttacgg ttgcgcaatc tgggcgggat tatcattatt gatttcatcg
    13441 atatgaataa tgaagatcac cgtcgccgcg tgctgcactc gctggagcag gcgctgagca
    13501 aagatcgggt gaaaaccagc gttaatggtt tttcggcgct ggggctggtg gagatgacgc
    13561 gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa cgaatgccca acctgccacg
    13621 gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga aatcatgcgc gagattgttc
    13681 gtgtccacca tgcttacgac tccgaccgtt tcctggtcta tgcttctccg gcagtagccg
    13741 aagccttgaa aggcgaagag tcacactcgc tggcggaagt ggaaattttc gttggcaaac
    13801 aggttaaagt acaaattgaa ccgctctata accaggagca gtttgacgtc gtaatgatgt
    13861 aaacagatgc tgggccgcca tccggcaaag ggtttttgag tcacattttt agcagacaag
    13921 gagtgacggg tgaggcgatt gccggggatt ttactgctta ctggagccgc gctcgttgtg
    13981 atcgctgccc tgctggttag cggcctgcgt attgctttac cgcatcttga cgcctggcgt
    14041 ccggaaatcc tcaacaaaat agaatccgcg actggcatgc cggtagaagc cagtcagctc
    14101 tcagccagct ggcagaattt tggtccgacg cttgaagcgc acgacatccg tgcagaacta
    14161 aaagatggcg gcgaattttc ggttaaacgc gttactcttg cgctggatgt ctggcagagc
    14221 ctgttacata tgcgctggca gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc
    14281 aacactccta tcaccagcgg cggtggtaat gatagcctgg aagccagtca catcagcgat
    14341 ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca gtgaagtcag tttcctgacg
    14401 ccatccggtc agcgcgccga gctggcgatc ccacagctca cctggctgaa cgatccacgt
    14461 cgacaccgtg cggaaggcct ggtaagcctc tccagcctta ccggacagca cggcgtgatg
    14521 caggtgcgta tggatttgcg cgatgatgag gggttgttaa gcaatggtcg cgtctggctc
    14581 caggcggatg acatcgacct gaagccgtgg ctcggtaaat ggatgcagga caatattgcg
    14641 ctggaaacgg cacagttctc ccttgaaggc tggatgacga tcgacaaagg cgatgtaacc
    14701 ggcggtgacg tctggctgaa acagggcggt gccagctggt tgggcgagaa ggaaacacat
    14761 acgctgtcgg tggataatct gactgcgcat attacgcgtg aaaatccggg atggcagttc
    14821 tctattcccg atacacggat cacgatggac ggcaaaccct ggccgagcgg agcattgacg
    14881 ctggcctgga taccggaaca ggacgttggc ggcaaagaca ataaacgcag tgacgaactc
    14941 cggattcgcg ccagtaatct ggagctggca ggcctggagg gcgtacgccc gctggtcgcg
    15001 aaactttcac ctgcactggg tgatgtttgg cgctccacac aaccgagcgg caagattaac
    15061 actctggcgc tggatatccc gcttctggcg gcagacaaga cccgttttca ggcatcgtgg
    15121 agcgatctgg cctggaagca atggaaatta ttaccgggtg cggaacactt ctccgggacg
    15181 ctttccggca gcgtagaaaa tggtttgctt actgcgtcga tgaagcaggc aaagatgcct
    15241 tacgaaacgg tattccgtgc gccactggaa atcgccgacg gccaggcaac gataagctgg
    15301 ctgaacaatg acaaaggttt ccagctggat gggcgtaata ttgacgttaa agccaaagcc
    15361 gtccatgcgc gcggcggttt tcgttacctg caacctgcta acgatgaacc ctggctgggt
    15421 attctggctg gcatcagtac cgatgatggt tcacaagcct ggcgctattt cccggaaaac
    15481 ttgatgggta aagacctggt tgattactta agtggcgcga ttcagggcgg tgaagcggat
    15541 aacgcgacgc tggtttatgg tggcaatccg caactcttcc cctataaaca caacgaaggt
    15601 cagtttgaag tgctggtgcc gctgcgcaac gcgaagtttg ccttccagcc ggactggcct
    15661 gcattaacta accttgatat tgaactggac tttattaacg acggtttatg gatgaaaacc
    15721 gatggcgtta atctgggcgg cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac
    15781 tcaaaagaaa aactgctgat tgacgctgac attaaaggtc cgggtaaagc cgttggccct
    15841 tactttgatg agacaccgct gaaagattcc ctgggtgcga ccctgcaaga actcaagctc
    15901 gacggcgatg tgaatgctcg cttacatctt gatatcccgc tgaacggcga gttggtaacc
    15961 gcgaaagggg aagtgacgct gcgtaataac agtctgttta tcaagccact cgacagcacc
    16021 ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg atctgcaaag tgaaccactg
    16081 acagcaagct ggtttaatca gccgttgaac gtggattttt ccaccaaaga aggggcaaaa
    16141 gcctaccagg tcgcggtgaa tctcaacggt aactggcaac cagcgaaaat cggctttctg
    16201 cctgcagcgg tgaacgaagc attaagtggc agcgtggcgt gggatggtaa agtgggcatt
    16261 gagctgcctt atcatgctgg cgcgacgtat aacgtagagc tgaacggcga tttgaagaat
    16321 gtgagcagtc acttaccttc accgttagcc aaacctgcgg gtgaaccact accggtaaac
    16381 gttaaggttg atggcaatct caacagcttt gaattaaccg gacaggctgg tgcggataac
    16441 catttcaata gccgctggtt gctcggtcaa aagctgacgc tcgatcgtgc tatttgggcg
    16501 gcagacagta aaacgctccc gccgttgccg gaacaaagtg gcgttgaact caatatgccg
    16561 ccgatgaatg gtgccgagtg gctggccctg ttccagaaag gcgcggcgga gagtgtcggt
    16621 ggtgcagcga gtttcccaca acacataacg ttacgtacgc ctatgttgtc gctgggaaat
    16681 cagcaatgga ataacctgag tattgtttcg caaccgacgg caaatggcac cctggttgag
    16741 gcgcaagggc gtgaaatcaa cgccacgctg gcgatgcgta ataacgcacc gtggctggcg
    16801 aatatcaaat atctttatta caacccaagc gtggcgaaaa ctcgtggtga ttcaacacag
    16861 tcatcacctt tcccgacaac ggaacgcatt aacttccgtg gctggccaga cgcacaaata
    16921 cgatgcgcag agtgctggtt ctgggggcaa aaattcggtc gtattgacag tgatatcacc
    16981 atttctggcg atacgttaac gctgactaat ggactgattg atactggttt ttcgcggctc
    17041 actgccgatg gtgaatgggt taacaatccg gggaatgaac gtacctcgct gaaaggaaaa
    17101 ctgcgcgggc agaaaattga tgccgccgca gaattttttg gtgtcacgac gcccgtacgc
    17161 cagtcgtcat ttaatgtgga ttacgattta cactggcgca aagcaccctg gcagccagat
    17221 gaggcgacgt tgaatggcat cattcatact caactgggta aaggcgaaat taccgaaatc
    17281 aataccggac atgccgggca attgctgcgc ttattgagcg tagatgccct gatgcgtaag
    17341 ctgcgttttg atttcagaga cacttttggc gaagggttct attttgactc cattcgcagc
    17401 accgcgtgga ttaaagacgg cgttatgcac accgacgaca cgctggtgga tggcctggaa
    17461 gcggacatcg ctatgaaagg gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg
    17521 gttgtcgcac cagagatttc tgcgacggtg ggtgtggctg cggcctttgc ggttaacccc
    17581 attgttggcg cggcagtgtt tgccgccagt aaagtgctgg gtccgctgtg gagcaaagtc
    17641 tccattttgc gctatcacat ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg
    17701 cgacaaccgc gtaaagaaaa agcgcaatga tttgacgagg gcgcgtaatt gccccaatct
    17761 catatgataa tcgttgccaa aggccaacga gccagaacat aaccgtaggc cggacagggc
    17821 gttcacgccg catccggcag ccgtaaaaaa tcctctactg cagtaactaa cgagtagcaa
    17881 aaacgatgag tctgaacctg gtaagtgaac aattgctagc ggcgaacggc ctgaaacatc
    17941 aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg ccttgattat ggcgatctct
    18001 attttcagtc gagctatcac gaatcctggg ttttagaaga ccgtattatt aaagatggtt
    18061 cttacaacat cgatcagggc gttggcgtgc gtgcaatcag tggtgaaaaa accggatttg
    18121 cttatgctga ccaaatcagc ctgctggcgc tggaacagag tgcacaagcg gcgcgcacca
    18181 tcgtccgtga tagtggtgat ggcaaagtac agacgctggg cgcggtagag catagcccgt
    18241 tatatacctc ggtagatccg ctgcaaagca tgagccgtga agagaagttg gatattctgc
    18301 gtcgcgtcga taaggttgcc cgcgaagcgg acaagcgcgt acaggaagtg actgccagcc
    18361 tcagtggtgt ctatgaatta attctggttg cggccaccga cggcacacta gcggcggatg
    18421 tccgtccgct ggtgcgtctt tccgtgagcg ttttggtcga agaagatggc aaacgcgaac
    18481 gcggtgccag tggtggcggc ggtcgttttg gttatgagtt cttccttgct gatctcgacg
    18541 gcgaagtccg tgcggatgcg tgggcaaaag aagcagtgcg tatggcgctg gtcaatcttt
    18601 ctgccgttgc tgcaccagcg ggcaccatgc cggtagtact tggcgcaggt tggccgggcg
    18661 tgctgttgca tgaagcggtg ggtcacggtc tggaaggcga cttcaaccgc cgtggcactt
    18721 cagtatttag tggacaggtc ggggagttgg tggcttcaga actgtgtacc gtggttgatg
    18781 atggcacgat ggtcgatcgc cgtggttcgg tggcgattga tgacgaaggt acgccaggcc
    18841 agtacaacgt gctgattgag aacggcattc tgaaaggcta catgcaggat aaactcaacg
    18901 cgcgtttgat ggggatgacg ccgactggca acggtcgccg tgaatcctac gctcatctgc
    18961 ccatgccgcg tatgaccaac acctatatgc tgccgggtaa atcgaccccg caggaaatta
    19021 ttgaatccgt tgagtacggt atctatgcac cgaactttgg tggcggccag gtggatatca
    19081 cctccggcaa attcgttttc tccacctcag aagcgtatct tattgaaaac ggtaaagtaa
    19141 ctaagccggt gaaaggcgca acgttgattg gttccggtat cgaaaccatg cagcagattt
    19201 cgatggttgg caacgacctg aaactggata acggcgtggg tgtctgcggt aaagaagggc
    19261 aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt tgataacctg actgttggcg
    19321 gtactgcgta ataatttcag tattttcaga aggataatta atccttctcc gtgtacgaac
    19381 ggtcccctcg cccttctggg gttagggtga ggggaacccg ttggcacagg tttgtacaac
    19441 gtaacagtac aatattaatt acttctcttt cccgcgcccg tgcatctcct gaaacaattt
    19501 accgacctca acaaaataat ccgttagcga gttgatcacg acttgtacct tcagcggcag
    19561 cttatctttt tcggtatata acgcataaac cgggcgtggg tctgactggt aacgtggcag
    19621 caggatctcc agttccccgc gattgatctc gttgatcacc cacatcaacg gcacgtaggc
    19681 gatcccagcg cctgcagtca gccagcgcac cagcgtcatc ggatcattag tcacaaatct
    19741 cccttgcggg atcaggcgag tcgagatccc ttccggtgcg atcagttcaa attcattgtc
    19801 gggtcgcacg ctgtattcaa gccatgaatg actacttaaa tcggcgggtt tttctggtat
    19861 gccgtattgt gtgagatagc tttttgcggc gcacaccacc attggcatcg cgcccagacg
    19921 gcgggaaaac aggctggaat cttgcaacgc gccgacgcgg atcaccacat ccagaccgtc
    19981 ggcaatcagg tcgggggcag gaattccggt aaccagattg acgctcaaac ctgggtattc
    20041 tttcagcatt tttgctgtca gcccggcgag aacattttgt gccatagttg aagaacagcc
    20101 aatgcgcagc gtcccgatgg gggtgttatt gaaggcatac agttgctcat gaacatcctg
    20161 cacttcatgg agcatccgac ggcagccctg gtagtaaatt ctaccggctt cggtcaggcc
    20221 aatgctgcgt gtgctacggt ttaacagctt tacctgcaac tcatcttcca gttttgacac
    20281 cgtctgactg atggacgaaa cgctcatctg tagctgtctg gcggcggcgg taaaagagcc
    20341 aaattcaact actttggcaa acaccgacat gcgttttagt cgttccatta ttcactctga
    20401 cttaaaagtg atttagatca cataatatag ataacagcat aacagttacg ttaatatatt
    20461 aaatatcaat ctacagcaat gttgctctcg cccggctttc catgcccttc tctgcggcga
    20521 cagatgctga aaataataac gcctgctctc tctatacaac caaggtcaac atgagtctgt
    20581 ttcccgttat cgtggtgttt gggctgtcct tcccaccgat atttttcgaa ttgcttttat
    20641 cactggcgat tttctggctg gtgcgccggg tacttgtgcc aacaggtatt tacgactttg
    20701 tctggcatcc ggcgttgttc aacaccgcgc tctattgctg cttgttttat ttgatatcgc
    20761 gactgttcgt ttgaggttga agtgaaaaca ctaataagaa aattctcccg tacggccatc
    20821 acggtcgtat tagtcattct ggccttcatc gcaattttta atgcctgggt ctattacacc
    20881 gaatccccgt ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac
    20941 gtttctggac tcattaccca ggtgaatgtt catgataacc agctggtgaa aaaaggacag
    21001 gtactgttca ccatcgacca gccgcgttat caaaaggcgc ttgaggaagc gcaagccgat
    21061 gttgcttatt atcaggtact ggcgcaggag aaacgccagg aggccggacg tcgtaaccgt
    21121 ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg ccaacaacgt actacaaaca
    21181 gttctgcatc agttagcgaa agcgcaggcg acccgcgatc tggcaaaact ggatcttgaa
    21241 cgtacagtca tccgcgcgcc agcagatggc tgggtgacca acctcaacgt ctataccggt
    21301 gagtttatta ctcgaggatc aacggcggtt gcgctggtga aacagaactc cttctatgta
    21361 ctggcctata tggaagaaac taagctggaa ggggtgcgcc ctgggtatcg tgcggagatc
    21421 acgccgcttg gcagtaacaa agtgctgaaa gggactgttg atagtgttgc cgcaggggtc
    21481 accaatgcca gcagcacgcg tgacgacaaa gggatggcga ccatcgactc taaccttgaa
    21541 tgggtacgtt tggcgcaacg tgttccggta cgtattcgtc tcgacaacca gcaagagaac
    21601 atctggcctg cgggcaccac tgctacggtg gtagtcactg gcaaacaaga tcgcgacgaa
    21661 agtcaggatt cgttcttccg taaaatggct catcgcctgc gtgagtttgg ttaatcacga
    21721 tgggtatttt ctccattgct aatcaacata ttcgctttgc ggtaaaactg gcgaccgcca
    21781 ttgtactggc gctgtttgtt ggctttcact tccagctgga aacgccacgc tgggcggtac
    21841 tgacagcggc gattgttgcc gccggtccgg cctttgctgc gggaggtgaa ccgtattctg
    21901 gcgccattcg ctatcgtggc tttttgcgca tcatcggcac atttattggc tgtattgccg
    21961 gactggtaat catcattgcg atgatccgag caccattatt gatgattctg gtgtgctgta
    22021 tctgggccgg tttttgtacc tggatatcct cgctggtacg aatagaaaac tcgtatgcgt
    22081 gggggctggc cggttatacc gcgctgatca ttgtgatcac cattcagccg gaaccattgc
    22141 ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt tatcggtatt gtgtgtgcaa
    22201 ttatggcgga tttgctcttt tctccgcgat cgatcaaaca agaagtggat cgagagctgg
    22261 aaagtttgct ggttgcgcaa tatcaattaa tgcaactctg tatcaagcat ggcgatggtg
    22321 aagttgtcga taaagcctgg ggcgatctgg tgcgacgtac tacggcgtta caaggtatgc
    22381 gcagcaacct gaatatggaa tcttcccgct gggcgcgggc caatcgacgt ttaaaagcga
    22441 tcaatacgct atcgctgacg ctgattaccc aatcctgcga aacttatctt attcagaata
    22501 cgcgcccgga attgatcact gatactttcc gcgaattttt tgacacgccg gtagaaactg
    22561 cgcaggacgt ccacaagcag ctcaaacgcc tgcggagagt gatcgcctgg accggggaac
    22621 gggaaacgcc tgtcaccatt tatagctggg tcgcggcggc aacgcgttat cagcttctca
    22681 agcgcggcgt tatcagtaac acaaaaatca acgccaccga agaagagatc ctgcaaggcg
    22741 aaccggaagt aaaagtagag tcagccgaac gtcatcatgc aatggttaac ttctggcgaa
    22801 ccacactttc ttgcattctg ggcacgcttt tctggctgtg gacgggctgg acttccggca
    22861 gtggtgcaat ggtgatgatt gcggtagtga cgtcactggc aatgcgtttg ccgaatccac
    22921 gcatggtggc gatcgacttt atctacggga cactggcagc gctaccgtta gggctgctct
    22981 actttttggt gattatccct aatacccaac agagcatgtt gctgctgtgt attagcctgg
    23041 cagtgctggg attcttcctc ggtatagaag tacagaaacg gagattgggc tcgatggggg
    23101 cgttggccag caccataaac attatcgtgc tggataaccc gatgactttc catttcagtc
    23161 agtttctcga cagcgcatta gggcaaatcg tcggctgtgt gctcgcgttc accgttattt
    23221 tgctggtgcg ggataaatcg cgcgacagga ctggacgtgt actgcttaat cagtttgttt
    23281 ctgccgctgt ttccgcgatg actaccaatg tggcacgtcg taaagagaat cacctcccgg
    23341 cactttatca gcagctgttt ttgctgatga ataagttccc gggggatttg ccgaaatttc
    23401 gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg tgatgcgccg attccggtta
    23461 acgaggattt atcggcgttt caccggcaaa tgcgccgcac agcagaccat gtgatatctg
    23521 cccgtagcga tgataaacgt cgtcggtact ttggccagtt gctggaagaa ctggaaatct
    23581 accaggaaaa gctacgcatc tggcaagcgc caccgcaggt gacggaacca gtacatcggc
    23641 ttacgggtat gctccataag tatcaacatg cgttgaccga tagttaagtc aaaaccggcg
    23701 cccgaagcgt cggttttttc atggctatac ttagcgatga acggcagaac tcgccgcgaa
    23761 acgtgacggt ggcaacagat gaatatttat acctttgatt ttgatgagat tgagagtcag
    23821 gaggattttt atcgtgactt tagccaaacc tttggtctgg cgaaagataa ggtacgcgat
    23881 ctcgactcac tatgggatgt gttaatgaac gatgtcctgc cgctaccact tgagattgaa
    23941 tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg cgttaatatt gctgtttgat
    24001 gaggcagagg aagagctgga agggcatttg cgttttaatg ttcgtcatta gcgcaaaaaa
    24061 agcccccgaa ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc
    24121 agccgtagcg tgccaggtgt caccactacg agcttcagta atctggtagg ccgttgcgcc
    24181 tttctcttcc gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc
    24241 acttacggat acggtcccga ttgcttcacg attttgtgct tgtgcagcat caatggagtc
    24301 ggcagcgaat gcaccgaaag agagaacaga aagcacactt aatgcagcaa cagtggtttt
    24361 gattttcatg atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat
    24421 atcacaaaat caagtataca ctaattattg aactaa
//
LOCUS       DAZFRG010000051        24449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFRG010000051 DAZFRG010000000
VERSION     DAZFRG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.051"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..2307
                     /gene="katG"
                     /locus_tag="ACHKUC_001603"
     CDS             127..2307
                     /gene="katG"
                     /locus_tag="ACHKUC_001603"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO6617878.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2401..3306
                     /gene="yijE"
                     /locus_tag="ACHKUC_001604"
     CDS             2401..3306
                     /gene="yijE"
                     /locus_tag="ACHKUC_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO6617879.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3333..3950)
                     /locus_tag="ACHKUC_001605"
     CDS             complement(3333..3950)
                     /locus_tag="ACHKUC_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO6617880.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(4226..5329)
                     /gene="gldA"
                     /locus_tag="ACHKUC_001606"
     CDS             complement(4226..5329)
                     /gene="gldA"
                     /locus_tag="ACHKUC_001606"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO6617881.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5340..6002)
                     /gene="fsa"
                     /locus_tag="ACHKUC_001607"
     CDS             complement(5340..6002)
                     /gene="fsa"
                     /locus_tag="ACHKUC_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6617882.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAFGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(6014..8515)
                     /gene="ptsP"
                     /locus_tag="ACHKUC_001608"
     CDS             complement(6014..8515)
                     /gene="ptsP"
                     /locus_tag="ACHKUC_001608"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6617883.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLVEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     AHHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8824..9903
                     /locus_tag="ACHKUC_001609"
     CDS             8824..9903
                     /locus_tag="ACHKUC_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO6617884.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            9918..10238
                     /locus_tag="ACHKUC_001610"
     CDS             9918..10238
                     /locus_tag="ACHKUC_001610"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6617885.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10289..12586
                     /locus_tag="ACHKUC_001611"
     CDS             10289..12586
                     /locus_tag="ACHKUC_001611"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6617886.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12552..13430
                     /locus_tag="ACHKUC_001612"
     CDS             12552..13430
                     /locus_tag="ACHKUC_001612"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO6617887.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            13432..13773
                     /locus_tag="ACHKUC_001613"
     CDS             13432..13773
                     /locus_tag="ACHKUC_001613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6617888.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13760..14611)
                     /locus_tag="ACHKUC_001614"
     CDS             complement(13760..14611)
                     /locus_tag="ACHKUC_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6617889.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14837..16570)
                     /locus_tag="ACHKUC_001615"
     CDS             complement(14837..16570)
                     /locus_tag="ACHKUC_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6617890.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALPYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(16753..19404)
                     /gene="ppc"
                     /locus_tag="ACHKUC_001616"
     CDS             complement(16753..19404)
                     /gene="ppc"
                     /locus_tag="ACHKUC_001616"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO6617891.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(19706..20857)
                     /gene="argE"
                     /locus_tag="ACHKUC_001617"
     CDS             complement(19706..20857)
                     /gene="argE"
                     /locus_tag="ACHKUC_001617"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO6617892.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            21011..22015
                     /gene="argC"
                     /locus_tag="ACHKUC_001618"
     CDS             21011..22015
                     /gene="argC"
                     /locus_tag="ACHKUC_001618"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO6617893.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            22023..22799
                     /gene="argB"
                     /locus_tag="ACHKUC_001619"
     CDS             22023..22799
                     /gene="argB"
                     /locus_tag="ACHKUC_001619"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO6617894.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRQLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTNEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQSIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            22860..24233
                     /gene="argH"
                     /locus_tag="ACHKUC_001620"
     CDS             22860..24233
                     /gene="argH"
                     /locus_tag="ACHKUC_001620"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO6617895.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
BASE COUNT         5521 a         6245 c         6828 g         5855 t
ORIGIN      
        1 ttgatctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
       61 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
      121 acattgatga gcacgtcaga cgatatccat aacacgacag ccaccggcaa atgcccgttc
      181 catcagggcg gtcacgacca gagcgcaggt gggggcacaa ccacgcgcga ctggtggcca
      241 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
      301 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
      361 aaagccctgc tgacagaatc tcaaccgtgg tggccagccg actggggcag ctacgccggt
      421 ctgtttattc gtatggcctg gcacggcgcg ggtacttacc gttcaatcga cggtcgcggc
      481 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
      541 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggcca gaaaatctcc
      601 tgggccgacc tgtttatcct cgcgggtaac gtggcgctgg aaaactccgg cttccgtacc
      661 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
      721 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
      781 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaacca
      841 ctttcggcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa tgacgaagaa
      901 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc tggtccgaca
      961 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     1021 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agtggtctgg
     1081 acccagacgc cgacccagtg gagcaactat ttcttcgaga acttgttcaa gtatgagtgg
     1141 gtacagaccc gcagcccggc tggcgcaatc cagtttgaag cggtagacgc accggaaatt
     1201 atcccggatc cgtttgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     1261 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     1321 gcgttcaacg aagcctttgc tcgtgcctgg ttcaaactga cgcacaggga tatggggccg
     1381 aaatctcgtt acatcggacc ggaagtaccg aaagaagatc tgatctggca agatccgctg
     1441 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     1501 gattctggtc tgtctgttag tgagctggta tcggtggcct gggcgtctgc ttctaccttc
     1561 cgtggtggcg acaaacgtgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgt
     1621 gactgggatg tgaacgccgc agccgttcgt gctctgccag ttctggagaa aattcagaaa
     1681 gagtctggca aagcttcgct ggcggatatc atcgtgctgg caggtgtggt tggcgttgag
     1741 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
     1801 gcgcgtcagg atcagactga cattgagatg ttcgaactgc tggagccaat tgctgacggt
     1861 ttccgtaact atcgcgctcg tctggacgtt tccaccaccg agtcactgtt gattgataaa
     1921 gcacagcaac tgacgctgac cgcgccggaa atgactgcgc tggtgggcgg gatgcgcgta
     1981 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
     2041 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
     2101 tcgaaagaac tgttcgaagg ccgtgaccgc gaaactggcg aagtgaaata cacagccagc
     2161 cgtgcggatc tggtctttgg ttctaactct gtcctgcgtg cggtggcgga agtctacgcc
     2221 agcagcgatg cccacgagaa gtttgttaaa gacttcgtgg cagcgtgggt gaaagtgatg
     2281 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcatgcag
     2341 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
     2401 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
     2461 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
     2521 ttcaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
     2581 ggtcgcggaa tgcgcccgac gccgtttaaa tacaccttag ccattgccct gttacaaacc
     2641 tgcgggatgg ttggcctggc acagtgggcg ctggtcagcg gcggagcggg gaaggtggca
     2701 atcctgagct ataccatgcc gttctgggtg gtaattttcg ccgcgttgtt tctcggtgaa
     2761 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
     2821 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
     2881 ggtgtgagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg ccatccgcgc
     2941 gtggatttat tgtcgctaac atcctggcag atgctgtatg cggcgctggt gatgagcgtg
     3001 gtcgctttac tggtgccgca acgtgaaatt gactggcaac ccaccgtgtt ctgggcgctg
     3061 gcctacagtg cgattctggc gacagcgctg gcgtggagct tatggttgtt tgtattgaaa
     3121 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
     3181 ttttcctggt ggctgctcgg tgagaatccg ggggcagttg aaggtagcgg tattgtgctg
     3241 attgtgctgg cactggcgct ggtgagccgt aagaaaaaag aagccgtcag tgtaaaaagg
     3301 atctgaattt tttcttcatg tggggagatc tcttatttag caaaataccg atagtgcccc
     3361 accatccgcc agctaaacag tacatcttct tcctgcgcgc ctgcgccaat gttatgtatc
     3421 accagcggcg taccgtcgcg ggcgaagcca tctgacacca ccccaatatg tgccagcccg
     3481 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctggtg
     3541 gggcgtgttt tatcgtggcg ggtaaaccag gtttccagat taggcacccg acggtgatcg
     3601 atgttgctgt ccgggcgctt taactgccat ttttgcgggt acgcagcaaa attcttcgcc
     3661 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
     3721 acatcggaac atacgccgcg ttcttgcggg acatcaccgc caggataggt aagctgcaca
     3781 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcgccgtc tgcgatggca
     3841 agattggtgt tagcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
     3901 taactggtaa ataccgtcag aagactgaat aacgccagtg aagctttcat ctgtttcgtc
     3961 cgtgaaaata agagtcacag attcgacctt ccgggctaac gattgttctg tggagaaaat
     4021 gtccagaagc ggataaaaaa acacccgaac aaggtggaag tttcagatta ctggcacagc
     4081 gtgccggatg cggcgtgaac gccttatccg gcctacaaaa gtacgtaaat ttactatatt
     4141 gcagagattg cgtaggcctg ataagcgtag cgcatcaggc aattttgcgt tcaaactccc
     4201 ggacaagccg ggagtttgga gtaggttatt cccactcttg caggaaacgc tgaccatact
     4261 ggtcggctac cagcagtgcg gcgtaaacct gatctggcgt cgcgccgcca ggcatgttgt
     4321 ggatggtttc accttctgca catgccgctt ctgccacaat tcgcattttc gccgggacat
     4381 cttctttaat atccagttga gcgagagtta ttggcaaacc taccgcatgg ctaagcgcag
     4441 ctacggtttc gatttcctcc accggcgcgt tttccagaac cagctgcgtc agcgtaccga
     4501 atgccacttt ttcaccatga taatagtgat gcgcgtccgg gatagcggtc aggccgttat
     4561 gtaccgcgtg tgccgccgcc agcccgccgc tttcaaaacc aacgccactc aaataggtgt
     4621 tcgcttcaat cacgcgctcc agcgccggag tcactacatg ctgttcggcg gcaagcatcg
     4681 ctttttcgcc ttcttccagc agggtgttgt agcacagttc agccagtgcc agcgctgcct
     4741 gagtgcactt gccacccgcc atggtggtcg cgccgctacg ggaacaggca cgcgcttcaa
     4801 accaggttgc cagcgcatcg ccaatacccg ccgctaacag acgcgcgggc gcgccagcga
     4861 cgattttggt gtcgacaatg accatattcg ggttatttgg caacagcaga tagcggtcaa
     4921 actcaccctc atcggtgtag ataacagaca gtgcgctgca cggtgcgtcg gtagaagcga
     4981 tagtcggtgc gatcgctacc ggaacaccca tgaaatgtgc cagtgctttg gcagtatcga
     5041 gggtttttcc gccaccgata ccgagaattg cgccacactg cgcagtctcc gcgatgccac
     5101 gcagacggtc gatctcattt tgcgaacatt caccgccaaa cggcgcaatt tctactacca
     5161 gtccagcatc tttaaagctt ttctcgacag tggattgagc aaaacctaaa acaaatttgt
     5221 cacccaccac taaccagcgt tccgccagtg gcttcaggta ctcgcccaga cgattaatta
     5281 catctgcgcc ctggatgtat ttacctggtg attgaataat gcggtccata attactcctt
     5341 tagagatgag tagtgccaaa tgcggcattc cagtcgtgct cgaacttctc tatagctgac
     5401 tctaccgcag gggtgttaag catttgttgc gctacatcta agggcagtgt gatggattca
     5461 catccagcca gcaaacaatc cagcgcctga cgtggtgttt taaagctggc agccagcacc
     5521 atgctttctg gcgcatgcat ttccagtaac gcttgcaact cctgtacagt acgaatgccg
     5581 tcaccgccct gggcatctac gcggttaacg tatggagcaa cgtatttggc tccagccagc
     5641 gccgccagta atccttgcgc ggcgctgtac actgcggttc ccagcgtagt aatgccttct
     5701 ttcttcagca ttttaattgc tgccagacct tcagaggtta ccggaatttt caccacaatg
     5761 cccggaatag cgtcgcgcag gcgtttcgct tctttcacca taccctgcgc gtcgcggctc
     5821 atggtctgag caaacagaat tccctcatca ccgaacgctt tttgcaggcg cggcagcact
     5881 tcccagatgg actccttgct ggcagcgata atgctcgggt tagttgtcac cccggcaatc
     5941 gggaatatgc gtgccagacg ttcgacttct gcgacgttag cggtgtccag atacagttcc
     6001 atgatgtttc cttttacagt tccagttcat gttgcagcag gctggcgata gcgtcggcag
     6061 aggcggcgtt aaccagcgca ttacggaatt cttcgtgcat gatgcgacga gcgaggcgcg
     6121 agaaaatgcg catatgttga tcgcccgcgg cgtgtttgtt cagggttaac ataatgatga
     6181 attgcgcttc atcatcgccc cagcgcactg gcgcttgcag acgcgctacg ctgatggtgg
     6241 attgctcaat gtgttccgat ttactgtggg gaatggcgaa actaaagccc agtccggtag
     6301 agaaaacggc ctcgcgcgcc cacaagtcgg cttccagttt acgcggatag cggcagcggc
     6361 ccgccagcag caggttatcg gtcatgcctt tgagcacttc ttctttgctg cgccagtcgc
     6421 tttccagtgt gatgcactcg gcggtgacca gcggtgcgtc ctgttgggtc atgcggaatt
     6481 gcgccagcag gtgttccact tccagcgaag tacggcaggc cattgcctgg ttgagcaact
     6541 ggcggcactc acggctatca agttgcgcca tccgcgcttt cgccgccgga attgatggtg
     6601 cgctcatgct gagttcatcc agccctaaac cgaccagcaa cggcagcacg gaacctttcg
     6661 ctcccagttc accgcacaga ccaatccatt tgccctggcg atgcaccgcc tgcacggcgt
     6721 aatcgagcgc ccgcaagaag gccggattca ggctgttgta gtgacgagtc accttagcgt
     6781 tatcgcgatc cactgccagt agatattgcg tcaggtcatt actaccaata ctaaagaaat
     6841 caatctcttc gcagcattga tcgatgatga acatcaccga cggcacttcc agcatgatgc
     6901 cgagctggat cttctcatca aacggaatgt gttcgttacg cagttgctgt ttggcttccg
     6961 ccagtttttc tttcacccat aagatctctt ccattgagga gatcatcggg atcatgattt
     7021 tcaggctgcc gtgagcagag gcgcgcagga tcgaccgtag ttgtgtggta aacaacgacg
     7081 cgtattcttc ataaatacgc accgcgcgat aaccgaggaa cgggttagct tctgccggaa
     7141 tgttcagata atcaacgggt ttatcaccgc caatgtccat agtgcgcaca ataatgctgc
     7201 gtccgttggc agactccagc gcctgacaaa aaatgttgta cagctcgctt tcacccggtg
     7261 cactggtgcg atccatatag agcatttcag tgcggaacaa accaacgcct tccgcaccat
     7321 tgccgaatgc ggcctgcgct tccacggagt gagcgatgtt agcggcaatt tcaatgcgga
     7381 taccgtctgc ggtacgcgct tgctgggtca gccagacacg ctgttgctca cgcagagcgt
     7441 cctgtacgcg ggcttcttgc tgataataac gggccacgga ttcccctggc tcaaccacaa
     7501 tcgcccctgc gttgccgtcg atataaatcg tttgatgttg ccacggagta agggcatcaa
     7561 tatccacacc taccagcgtc ggaatgttga acgaacgggc aaggatcacc gtatgtgagg
     7621 tggtgccgcc acttttcagt aatagacctt tgaggtgatt tttatccagt tcgaggaact
     7681 ggctgggggt cagttcatcc gccatacaaa tggcgggctg cgtcagtttg cccggtgccg
     7741 ggaagcgttg ctcaccgtag atttgctgga gtaactggaa gcagacgtcg cgtacgtcca
     7801 gggcgcgttc ttgcaggtag ctgctgctgg aacgggaaaa ctcttcgcag aagtggtgtg
     7861 cactggcgac aatcgcttcg gcacagctta atccggcgct gacacctgcc agtaaatgtt
     7921 cgcgcaggga agtatcgcca gccagggatc ggtgcgcttc cagaatcgcg ctggtagcac
     7981 cgtcgctatc cagcagacga aactctatgt ttttcagtac cagcgtcaga ccgttttcca
     8041 gtgcggattg ttcggcgtca acgcctttgg ctgcgggaag attacccagc gcattgagat
     8101 ctaaagaaga gatcggcgtc agaatgccgc ctgcgctacc gctgcacacg gtgcgagcac
     8161 ggataatttg cggattcaga ttggtcagtg aaatcggcag tggttccagt tcgtcagatt
     8221 ttacttccac cagcggtgcg tcgcagtggg ggaattcatc gcgcagccac tggcttaaac
     8281 gctggtgcgc ttcctgttcg tcggccccgg aaatcagtaa ctggcagtta tcgcccacca
     8341 gcgtatcggt gccaatcagc gcaagggcgc ttttggcgtt gcccttgcgg tcagtgcgca
     8401 ggttatgcca ctcaatttgt gatgaaaaag tattacacag cgtttcaacg tggcttgccg
     8461 gacgcgcatg cacgccgtta ggtagctcac aaataaattc cacaatcagg gccattgcct
     8521 ttctcccggt acggttttct gtttacagga taagagcgca cggcaacggc ctgccatgtg
     8581 acaaatctgc caaaagctgg acaaatgtaa tgtaaccgtc aatttgcgac gcgtctcaca
     8641 agacgccgtt ttgcggcgta cttccggatt atcgcaagtt atgaggcgga tcgcattttt
     8701 gtactgatat tacaaaagtc cagtaaatgg cctttttatc cactgtttgc tccgcctgcg
     8761 attgcctatt gtttcttcca tcaaaaatat ggacatgggc tttcgcaagc ccgggagcag
     8821 cttatgaatg agttggtgca gatcctgaaa aatacccgtc agcatttaat gacgggcgtt
     8881 tcacacatga ttcccttcgt ggtatcgggc ggtattttgc tggcggtttc cgtcatgttg
     8941 tatggcaaag gcgcagtgcc ggatgccgta gccgatccga atctgaaaaa actgtttgat
     9001 atcggcgttg caggtttgac gctgatggtg cctttcctcg ccgcttacat cggttattcc
     9061 attgcagaac gttctgcgct ggctccatgc gctatcggtg cctgggttgg taacagcttt
     9121 ggcgcgggct tctttggtgc gctgatcgcc gggattatcg gcggcatcgt ggtgcattac
     9181 ctgaagaaaa ttccggtgca taaagttctg cgctcggtga tgccaatctt catcattcca
     9241 atcgtcggca cactgattac cgctggcgtc atgatgtggg ggctgggcga gccggtgggg
     9301 gcgttgacca acagcctgac ccagtggctt caggggatgc agcagggcag cattgttatg
     9361 ctggcggtga tcatgggtct gatgctggcg ttcgatatgg gcggcccggt taacaaagtg
     9421 gcctatgcct tcatgctgat ttgcgttgct cagggtgttt ataccgtggt ggctattgcc
     9481 gctgttggga tttgtgttcc accgctgggg atggggctgg cgacgctgat tggtcgtaaa
     9541 aatttctccg cagaagagcg cgaaaccggc aaggcggcac tggtgatggg ctgtgttggc
     9601 gttactgaag gggcgattcc tttcgccgct gccgatccgc tgcgtgtcat tccttccatc
     9661 atggtcggtt cagtttgtgg tgcggtaact gcggcgctgg tcggtgcgca gtgctatgca
     9721 ggctggggtg gtctgattgt gctgccggtg gttgaaggta agttgggtta catcgcggca
     9781 gtggctgtcg gggcggttgt gacggcagtc tgcgtgaacg tgctgaaaag tctggcgcgt
     9841 aaaaatgggt cttcaactga agagaaggaa gacgacctgg atctggattt tgaaattaat
     9901 taattgagga acgagccatg acgaaaatta tcgcagtaac cgcatgtcct tcaggtgttg
     9961 cccatactta tatggcggca gaggcgctgg aaagcgccgc gaaagcgaaa ggctgggaag
    10021 tgaaagtaga aacccagggg tcgattggtc tggaaaatga actgactgcg gaagacgtgg
    10081 caagcgccga tatggtgatt ttgaccaaag atatcggcat caaatttgaa gagcgttttg
    10141 ctggcaagac tatcgtgcgt gtcaacatca gcgacgcggt aaaacgtgct gacgccatta
    10201 tgagcaaaat tgaagctcac ctggcgcaaa ccgcttaagt tcttatcccg cccgcaaggg
    10261 cgggttcgct ttcccacagg agttcctcat gacgaatcgt atctctcgcc tcaaaactgc
    10321 actgtttgcc aatacccgtg aaatctcgct ggagcgggcg ttgctttata ccgccagcca
    10381 tcggcaaacc gaaggggagc cggtgataat gcgtcgggcg aaagcaacag cgtatatcct
    10441 tgaacacgtt gaaatttcga ttcgtgatga agaactgatt gccggtaacc gcaccgtaaa
    10501 accacgcgcc gggattatgt cgccggaaat ggacccttac tggctgctga aagagctgga
    10561 tcaattcccg acgcgtccgc aggaccgctt tgctatcagc gaagaagata aacgtatcta
    10621 ccgcgaagag ttgttcccgt actgggaaaa acgttcgatg aaagatttca tcaacgggca
    10681 gatgacggat gaagtaaaag ccgcgaccag cacgcagatt ttcagcatca accagacgga
    10741 taaaggccag gggcacatta ttattgatta cccgcgcctg ctgaatcacg ggctggggga
    10801 gctggtggca cagatgcagc aacattgtca gcaacagccg gagaatcact tttatcaggc
    10861 agcgctgtta ctgctggaag cctcgcagaa acatattttg cgttacgccg aactggcgga
    10921 aacgatggcg gcaagctgca ctgatggccc gcgtcgcgaa gaactgctga ccattgcgga
    10981 gatctcccgc cataacgccg aacataagcc gcagacgttc tggcaggcgt gccagttatt
    11041 ctggtacatg aacatcattc tgcaatacga atccaacgcc agttcgctat cgttggggcg
    11101 cttcgaccag tatatgttgc cgttctatca gacatcatta acccagggcg aagatccggc
    11161 attcctgaaa gaactgctcg aatctttatg ggtgaaatgc aacgacatcg tgctgttgcg
    11221 ctccaccagt agcgcgcgtt atttcgccgg tttccctacc ggctataccg cactgctcgg
    11281 cgggttaacc gagaacggac gtagcgcggt gaacgtgctt tcgttccttt gccttgacgc
    11341 ttatcaaagc gtgcaattac cgcaaccgaa cctcggcgtg cgcactaacg ccttgatcga
    11401 cacccctttc ctgatgaaaa ccgcagaaac cattcgcctc ggcaccggta ttccgcaaat
    11461 ctttaacgat gaagtggtag tgccagcgtt cctcaaccgt ggcgtttcgc tggaagatgc
    11521 gcgcgactat tccgtagtgg gctgtgtgga attatctatt cccggcagaa cctacggctt
    11581 gcatgacatc gcgatgttta acctgctgaa agtgatggaa atctgcctgc atgaaaatga
    11641 aggcaacgct gcgctgactt atgaaggttt actggaacag atccgcgcca agatcagcca
    11701 ctacatcacc ctgatggttg agggcagcaa tatttgtgat atcggccatc gcgactgggc
    11761 tcctgtaccg ctactctcgt cttttatcag cgattgtctg gaaaaaggtc gcgatattac
    11821 cgatggcggc gcgcgttata acttctccgg cgtacagggg atcggtatcg ccaacctgag
    11881 cgattctctc catgcgttga aagggatgat ttttgatcaa cagcgtttaa gttttgacga
    11941 attgctgtcg gtattaaaag ccaacttcgc cacgccagaa ggcgaaaaag tccgcgctcg
    12001 cttaattaac cgcttcgaga aatacggtaa cgatatcgac gaggtggata acatcagcgc
    12061 cgaactgtta cgccactact gcaaagaagt ggaaaaatac cagaacccgc gcggcggcta
    12121 cttcacgccg ggatcgtata ccgtttctgc ccacgtcccg ttgggatcgg tggttggcgc
    12181 gacgccagac ggtcgttttg ccggggaaca actggcagac ggtggcttgt cacccatgct
    12241 gggtcaggat gcacaagggc caacggcggt actgaaatca gtcagtaagc tcgataatac
    12301 gctgctgtct aacggtacgt tgctgaacgt gaaattcact ccggcgaccc tggaaggtga
    12361 agcgggatta cgcaaactgg ccgacttctt acgggcgttt acccagctta agttgcagca
    12421 tattcagttt aacgtggtga acgccgacac gttgcgggaa gcgcaacagc gcccacaaga
    12481 ttatgccggg ctggtggtgc gcgttgccgg atacagcgcc ttctttgtcg aactgtcgaa
    12541 ggagatccag gatgacatca tccgccggac agcgcatcag ctgtaacgtt gtggaaacgc
    12601 gccgcaatga tgtggcgcgc attttcaaca ttcagcgtta ttcactgaat gacggcgagg
    12661 gcattcgtac ggtggtcttt tttaaaggct gtccgcatct ttgcccatgg tgtgctaatc
    12721 cggagtcgat ctccggcaaa atccagacgg tacgcagaga ggcgaaatgt ctgcactgtg
    12781 cgaaatgttt gcgtgatgcg gatgaatgcc cctccggggc gtttgaacgg attggtcgcg
    12841 atatcagcct tgacgctctg gaacgggaag tgatgaaaga tgacattttc tttcgcacgt
    12901 ccggcggtgg cgtcacgctt tctggcggcg aagtgttaat gcaggcggag tttgcgaccc
    12961 gttttttaca gcgactgcgg ctatggggtg tgtcatgcgc catcgaaact gccggagacg
    13021 cgccagccag caagctgtta ccgctggcga aattgtgcga tgaagtgttg ttcgatttaa
    13081 aaattatgga tgcggctcag gcgcgggatg tggtgaagat gaacctgcaa cgcgtgctgg
    13141 agaatctgcg tttgctggtg agtgagggtg tcaacgtgat cccgcgttta ccgctgatcc
    13201 ctggtttcac gctcagccgg gagaatatgc agcaggcgct ggacgtgctg atcccgctga
    13261 atatcaagca gatccatctg ttaccgtttc atcagtacgg cgagccgaaa taccgcctgc
    13321 tggggaaaac atggtcgatg aaagaggttg ctgctccgtc gtcagccgat gtggcaacga
    13381 tgcgtgaaat ggcagaacgg gccggatttc aggttaccgt gggaggttaa aatggcatac
    13441 ctggtggcag taaccgcctg cgtaagcggc gtggcgcata cttatatggc ggcggaacgg
    13501 ctggaaaagt tgtgccagtt agagaagtgg ggcgttagca ttgaaactca gggcgcgttg
    13561 ggaacggaga atcgtttagc ggacgaggat atccgccggg cggatgttgc gctgttaatt
    13621 acggatatcg agcttgccgg tgctgagcga tttgaacatt gtcgctacgt gcaatgcagc
    13681 atctacgctt tcctgcgtga gccgcagagg gtgatgagcg cagtgcgcaa agtgctttct
    13741 gcgccgcagc aaacccatct tattctggag tagtcggttt ttctgtcagt tggctgtggt
    13801 actgccgacg atattccgac ggcgagcgtt cggtattttt acgaaacaga cggcagaagt
    13861 agttgctgtc gacaaagccg caggcgtgcg ccacttcttt taccttcagg tcatagcctt
    13921 tcaataatgt cttagcgtgc tccagtcgcg tgtgattcag gtattcgtta aaaccaatgg
    13981 cccccgtttt ttgaaacagg tgcgagaggt aatttggcga aatatagaac gcctgtgcga
    14041 cagattcgcg ggtaagtgcg gaggcatagc gttcgtcgat ataatcacga atagcttcga
    14101 atagagcctg gctgcgtgag gcggtctgta tttggctgcc gagcaaatcg cggcagtggc
    14161 taagcaggct ggtgacgata agtctggccg tttgctgctc ctgcgactgc atttgcattt
    14221 cattgagcgt ttgtagcaga aaagaaccaa tgcgcgggcc gcgtcgggcg acgtgttgct
    14281 tcgccagatt ttgatattgt ttgccatccc attgcacaac gctgaagccg agttgctgtt
    14341 tgccaaacag cacgctaaag gtggtggcgg gggcttgcca ttgtggaaaa ttccagccac
    14401 cagcagggac gtacagcaca tcgccgggaa ctaacgctgc tccagcgccg gtatcaataa
    14461 actcaccttc cagcacaatt tccagccgtg gaaaatcgac ctgatacgca agatcgggca
    14521 ccgggccgtt gctgctggca aagtaaatct ggcgcaggga cagcgggccg ttgatcaggc
    14581 gggagagcag atagctgacg tcgtgataca tgttattccc ttaggtatgt agacgggaaa
    14641 acagtaactg gcattatgaa aaaaggccac ctgaaaagtg tggcctgatg ggagtgagat
    14701 gtaaacattg cggcagtttc agtcgacttg tattgccgga tgcggcgtaa acgccttatc
    14761 cggcctaccg ccgcgatctg taggccggat aagacgcgtc aagcgtcgca tccggcaaat
    14821 aacatctggc tgataattac tgattaccca cctgatcgcc atacggcaga gtgtcgtaat
    14881 cgatcagtgc gtttttctta tacgggttac caatccagcg ggtagtttct ttgaattgcg
    14941 gattcaccac tgaacgggtt ggatcgtaac cgtcgtaaga taagcccgcc aaatctgacc
    15001 aggtgtggat cagttccgcc aggctgtatt tacgatcaac atcctgtgag aaatcacgcg
    15061 gatgagtcgc ttgccatttt tctgacgtcc acagcaggaa cggaatggtg tacatatgac
    15121 gcgtcgggtt gtcttcatta cgcccctgag ttttgtgcgg cggcgtgtcg taaacctctt
    15181 caccgtggtc agagaaataa accaggaaac cgttcggatc ggtcgcttta aagtctttaa
    15241 tcaggctggc aaccacgtga tcgttgtaca ggttagcatt gtcataatca ttatatgact
    15301 ccagctcttc tgcgtttaat cccggtggaa catgctctgt attaccgtca aatttgccct
    15361 gatcttccgg ataacggtac ttgtacttga tatgcgtacc cagcagatga acgatgatca
    15421 gtttcttcgg cgcagggtcc ttcagcactt cctggaacgg cttcagcacg ttggtgtcat
    15481 attcacgcgc actctgcgta cgttgctggt tcatgtagta ctgcttgtcg gtctggcgcg
    15541 aaaataccgt cagcatggta ttgcgggcgg tcatcgtctg ctggttggtg atccagaagg
    15601 ttttataacc cgcctgtttc atcatgttca tcagcgacgg ctgcgtcaga tacagatccg
    15661 ggttcttttc attggcaaag gtcagcgcct gttgcaggat ttcaatggtg tacggacgag
    15721 acgtaaccac gttattaaac acggtcagat tcggatcggt tttatgcagc gcatccagct
    15781 ccggcgtggt ttcacgcgga taaccgtaca gactcatgcg tccacgctgg gtcgattcgc
    15841 caattaccag cactaaggtg cgcggttcgt tacccgattc atctttgaaa ttagccagcg
    15901 gcggcaaggc attattttca ttcagtagct ttgttagcga gtttagttgc tgacgatact
    15961 gataatagcc ggtcaggaat tgccacggtg cggcaggctc catacgcgat gccaggttat
    16021 ccaacgtttt ctcaaacggc ttgttcttga taaacgtatt catggcgatc ggatgcagaa
    16081 tcaagccata aagcagggca aaagagacaa cataacgcca cggctttgga atatagaccg
    16141 ggcgcaggcg tgtccacagc agaactgcca ccgccgtata gggcagcgcg ataagcacaa
    16201 ttttcaggct gaaatactgg cttaaatact cgctggcttc gttggtgttg gtttcgaaca
    16261 tcacaaacag aacgctctgc gagaactcct gaccgtagat gacgtaatag cacagcgccg
    16321 ccagagaggc cgcccatagc accacgccga tcactgcggc aataatttta atccgcttcg
    16381 gaaagaggaa taccgggatc aaccacagcg aactgaataa cagcgagtcg cgaatgccgt
    16441 tagtgccact ataaccactg atgtaaataa tggcctgtag cagagtagag aaaaaccaaa
    16501 agtagagcag tgcccaaccc agggctttcc agctaaaaag gggtttagcc tggacttctg
    16561 tggaatgcat agtaagaacc tgtcttgaaa aaaatatcgc cgaatgtaac gacaattcct
    16621 taaggatatc tgaagggata tccagatttt gaataaaatg cagacagaaa tatattgaaa
    16681 acgagggtgt tagaacagaa gtatttcaga aaaccctcgc gcaaaagcac gagggtttgc
    16741 aggagaggaa gattagccgg tattacgcat acctgccgca atcccggcaa tagtgaccat
    16801 taacgcctgc tcgacgcgag gatccggttc ctggccttct ttttctgcct ggcgggagcg
    16861 gtgcagcaac tcggcctgca atacgttcag cgggtcggtg taaatattcc gtagctgaat
    16921 agactctgca atccacggca gatcggccat caggtgagaa tcgttggcaa tcgccagcac
    16981 cactttgatg tcttcttctt gcagattgcg taactcttta cctaacggcc acagtgcttt
    17041 gtcgaccagg cgttggtcat agtattctgc cagccacagg tctgctttgg cgaagaccat
    17101 ctccagcatg ccgagacgcg tcgagaagaa tggccaatcg cggcacatgg cttccagctc
    17161 gctctgtttg ccatcttcga ccactttttg cagcgccgta cctgcaccca gccaggcggg
    17221 gagcatcagg cggttttgcg tccaggcgaa aatccacgga atagcgcgca gtgactcgac
    17281 gccgccggtc gggcgacgtt tcgccggacg tgaacccaac ggcagtttgc ccaattcttg
    17341 ttccggcgta gcggagcgga agtaaggcac aaaatctttg ttttcacgta cgtagccacg
    17401 gtagagatcg caggagatga ctgacagttc atccataatg cgacgccagc tctctttcgg
    17461 ctcaggtggt ggcagcaggt tggcttccag aatcgcccca gtgtacagcg acaggctgct
    17521 gacggtgatt tctggcagac catatttaaa gcggatcatc tcgccctgtt cggtcacgcg
    17581 caggccacct ttcaggcttc ctggcggttg tgacagcagc gccgcatggg caggtgcgcc
    17641 gccgcgacca atggaaccgc cgcgaccgtg gaacaacgtc agctcaatac ccgctttttc
    17701 gcaggttttg attaatgcat cctgtgcctg gtattgcgcc caggaagctg ccatcacgcc
    17761 cgcatctttt gctgagtcgg aatagccaat catcaccatc tgtttgccct gaatcaggcc
    17821 gcgataccag tcgatattca gcagctgggt catgacatcg ttggcgttgt tcaggtcatc
    17881 gagggtttca aacagcggag caaccggcat cgcaaacccg atacccgctt ctttcagcag
    17941 caggtggaca gccagcacgt cggacggcgt tttcgccatc gagatcacgt aggcggcaat
    18001 ggagccttgc ggcgcttcgg caatcacctg gcaggtatcg agcacttcgc gcgtttcggc
    18061 gcttggttgc cagttgcgcg gtagaagcgg acgtttggag ttcagttcgc ggatcaggaa
    18121 cgcctgtttg tcggcttctg accagctctc gtagtcgccg ataccgaggt agcgggtcag
    18181 ctcgcccagc gcttcggtat ggcgggtgct ctcctgacgg atatcaatac ggaccagcgg
    18241 tacgccgaaa catttcacgc ggcgcagggt gtcgagcaga tcgccgttgg cgataatacc
    18301 catgccacac gcctgaagtg actggtagca agcgtagagc ggttcccaca gttcttcgtt
    18361 ttgtgtcagc aggccttctg gttttggcag ttcttcgcct ttcaggcgcg cttccagcca
    18421 tgcctgtgtc gccatcaggc gagaacgcag gtttttcatc agatagcgat acggttctgc
    18481 ggcaccttct tcgccaacca gcgccagcag ttcaggggtc gcttcaacca tcgacagttc
    18541 agaaaccagc acctgaatat ctttcaggaa cagatcggtg gctttccagc gactgagcag
    18601 caggacgtgg cgggtgatat cggcagtgac gttcgggttg ccgtcgcggt caccgcccat
    18661 ccacgaggta aaacggaccg gaacaaattc gacgggcagt ttgtagccga gattctcttc
    18721 cagttgttcg ttcagttcgc gcaggtaatt cggtacgcct tgccacaggc tgttttctac
    18781 tacggcaaag ccccatttgg cttcatctac cgggcttgga cgcagcttac ggatttcatc
    18841 ggtatgccat gactgggcga tcaactggcg caggcggcgc atcagctggt tatgttcgta
    18901 gtcggcgata tctttgttat cgagctgttt taagcaggcg ttcacttcca ccattttgtg
    18961 gatcagtgta cgacgggtaa tttcggttgg gtgagccgtg aggaccagtt ccagcgacag
    19021 cgattccact gcttttttta tggtgtcttc gctcagttcc ggctggtttt tcagtttgcg
    19081 cagggtgcgg gcgatcactt ccgggttgct ggcagcttcg cctttcggcg aaatgctgtg
    19141 gtattgctcg gcggtgttgg ccaggttcag aaactgacta aacgcacgcg caacgggcag
    19201 cagctcgtcg ttcgacaaat tttgtaaggt ggtgagcaac tcctggcggt tagcatcatt
    19261 gccagcgcgt gaagatttgg acaacttacg gatagtttct acgcgttcaa gaatgtgttc
    19321 tcccaacgca tccttgatgg tttctcccag cactttgccg agcatactga cattactacg
    19381 caatgcggaa tattgttcgt tcatattacc ccagacaccc catcttatcg tttgatagcc
    19441 ctgtatcctt cacgtcgcat tggcgcgaat atgctcgggc tttgcttttc gtcgtctttt
    19501 ataaagccac gtaaaagcga tgacgtcaaa tgctgcgaaa tcgcttcagc aaacgaataa
    19561 atagcaggaa tttacgccat taaattcacg acgctttaaa taagcgtaac ttatgggaat
    19621 gttaaaaaat cgcctcgagt aacaccaaag gtgtaggtcg gataagatgc gcaagcatcg
    19681 catccgacat tgtttggcct acgttttaat gccagcaaaa atggtgaatc acctgggtga
    19741 tcagttcgcg ggtgggcttg ataaacctcg tctccagata ttcatcaggc tgatgagcct
    19801 gattaattga gccaggcccc agaaccagcg tcgggcataa cgtttgaata aacggcgctt
    19861 cggtacagta attcaccact tcggtctttg ctccgagcaa tttctcaacc acttcaacca
    19921 gttgatgatt cggtgggcat tcatagccag ggatcggcgg atgcagctcg tcgaccgtca
    19981 gacgacccgg ccagcgttcg ctcactggag ccaatgcatc gttgagcaaa ccgttaagtt
    20041 cattgagtgt catgcctggc agcggacgaa tatccatatg caactcacaa caagcgcaaa
    20101 tacggttaga tgcatcgccg ccgtggatat gcccgaggtt gagcgtaggg tatggcacgg
    20161 taaacgcgtc gtagtgataa cgttctttca ggttatcgcg caattgcaaa atatgcccta
    20221 tggcgtcgtg catcagttcg atagcgttaa ctccgcgtgc tggatcgctg gagtggcccg
    20281 actggccctg aatacggatg gcgttagaga tatgaccttt atgcgcgcgt accggttgca
    20341 gcgacgttgg ctcgccaatg atggcgcaat ccgggcgcag ggcggtagtt tcggcaaaat
    20401 aacgcgctcc ggccatactg gtttcttcat cggcagtcgc cagaatgtag agcggttttg
    20461 caagcttcgt gacgtcgaca tcgcgtagcg catcaaggat aaacgcaaaa aagcctttca
    20521 tgtcggcggt gcctaagccg taaagcttgc cgtcatgctc cgtcaacgta aatggatcgc
    20581 gcgtccagcg tccgtcatca aatggcaccg tatcggtatg ccccgccagc aacaaaccgc
    20641 cagccccctg tccgcaactg gccagcatat taaatttgtt gcgagttcct ggcacaggct
    20701 gtacttccac attgaagcct aaatccttaa accagtccgc cagcagagtg attaaatctg
    20761 cattgctttg atcgagtgcc tcttccgtgg cgcttattga aggtgtggca atcagagcac
    20821 ggtaaatctc gataaatggc ggtaatttgt ttttcattgt tgacacacct ctggtcatga
    20881 tagtatcaat attcatgcag tatttatgaa taaaaataca ctaacgttga gcgtaataaa
    20941 acccaccagc cgtaaggtga atgttttacg tttaacctgg caaccagaca taagaaggtg
    21001 aatagccccg atgttgaata cgctgattgt gggtgccagc ggctacgctg gcgcagagct
    21061 agtgacctat gtaaatcgcc atccgcatat gaacataacc gctttgactg tttcagcgca
    21121 aagcaatgat gcgggaaagt taatctccga tttgcatccg cagctaaaag gcatcgttga
    21181 tctgccgttg cagccgatgt cggatatcag cgagtttagc ccaggggtgg acgtggtgtt
    21241 tctcgccacc gcccatgaag tcagccacga tttagcgccg caatttcttg aagcaggctg
    21301 tgtggtgttc gacctttccg gcgcgtttcg tgttaacgat gtcgccttct atgaaaaata
    21361 ttacggcttt acccatcaat acccggaact gctggaacag gcagcctacg gtctggcgga
    21421 gtggtgcggt aataaattaa aagaagcgaa cttgattgcc gtgccaggct gttatccgac
    21481 ggcagcacag ctggcgctga aaccgttgat tgatgccgat cttcttgacc tcaatcagtg
    21541 gccggtgatc aacgccacca gcggcgtgag cggtgcagga cgtaaggcag cgatttcaaa
    21601 tagtttttgt gaagttagcc tgcaaccgta tggcgtcttt acccatcgcc atcaaccaga
    21661 gatcgccaca cacctcggtg ctgacgttat cttcaccccg catctgggca atttcccgcg
    21721 cggcattctc gaaaccatta cctgccgcct gaaaccgggt gtgagccagg tgcaagtggc
    21781 gcaagcgtta cagcaggcgt atgcccataa accactggtg cggctgtatg acaaaggcgt
    21841 tccggcgctg aaaaatgtcg ttggactgcc attctgcgat atcggttttg ccgttcaggg
    21901 cgagcatctg attattgtgg cgaccgaaga caacttactg aaaggcgcgg cggcacaagc
    21961 ggtgcagtgc gccaatattc gtttcggcta tgcggaaacg cagtctctta tttaagggtg
    22021 caatgatgaa tccattaatt atcaaactgg gtggcgtact gctggatagt gaagaggcgc
    22081 tggaacgtct gtttagcgca ctggtgaatt atcgcgagtc acatcagcgt cagctggtga
    22141 ttgtgcacgg cggcggttgc gtggtggatg agctgatgaa agggctgaat ctgccggtga
    22201 aaaagaaaaa tggcctgcgg gtgacgcctg cggatcagat agacattatc accggagcac
    22261 tggcgggaac ggcaaataaa accctgctgg catgggcgaa gaagcatcag attgcggccg
    22321 taggtttgtt tctcggtgac ggcgacagcg tcaaagtgac ccagcttgat gaagagttag
    22381 gccatgttgg actggcgcag ccaggttcgc ctaagcttat caacagcttg ctggagaacg
    22441 gttatctgcc ggtggtcagc tccattggcg taacaaacga agggcaactg atgaacgtca
    22501 atgccgacca ggcggcgacg gcgctggcgg caacgctggg cgcggatctg attttgctct
    22561 ctgacgtcag cggcattctc gacggcaaag ggcaacgtat tgccgaaatg accgccgcga
    22621 aagcagaaca actgattgag cagagcatta ttactgacgg catgattgtg aaagtgaacg
    22681 cggcgctgga tgctgcgcgc acgttgggcc gtccggtaga tatcgcctcc tggcgtcatg
    22741 cggaacagct tccggcactg tttaacggta tgccgatggg tacgcggatt ttagcttaag
    22801 ttttgttggc cggaggcgca gctttccggc attgaatttc aaaataagga aacagagtta
    22861 tggcactttg gggcgggcgt tttacccagg cagcagatca acggttcaaa caattcaacg
    22921 actcactgcg ctttgattac cgtctggcgg agcaggatat tgttggctct gtggcctggt
    22981 ccaaagccct ggtaacggtc ggcgtgttaa ccgcagaaga gcaggcgcaa ctggaagagg
    23041 cgctgaacgt gctgctggaa gatgttcgcg ccaggccaca acaaatcctt gaaagcgacg
    23101 ccgaagatat ccatagctgg gtggaaggca aactgatcga caaagtgggt cagttaggca
    23161 aaaaactgca taccgggcgt agccgtaatg atcaggtagc aactgacctg aaactgtggt
    23221 gcaaagatac cgttagcgag ttgctgacgg ctaaccggca gttacaatcc gctctggtcg
    23281 agaccgcgca gaacaatcag gacgcggtga tgccgggtta cacccacctg caacgcgccc
    23341 agccggtgac gttcgcgcac tggtgcctgg cctacgttga gatgctggcg cgtgatgaaa
    23401 gccgtttgca ggatgcgctt aagcgtctgg atgtcagccc gctaggctgt ggcgcactgg
    23461 caggaacggc ctatgaaatc gaccgtgaac agttagcagg ctggctgggc tttgcttcgg
    23521 cgacccgtaa cagtctcgac agcgtttctg accgtgacca tgtgttggaa ctgctttctg
    23581 ctgccgctat cggcatggtg catctgtcgc gttttgctga agatctgatt ttctttaaca
    23641 ccggcgaagc ggggtttgtg gagctttctg accgtgtgac ttccggttca tcattaatgc
    23701 cgcagaagaa aaacccggat gcgctggagc tgattcgcgg taaatgcggt cgggtgcagg
    23761 gggcgttaac cggcatgatg atgacgctga aaggtttgcc gctggcttac aacaaagata
    23821 tgcaggaaga caaagaaggt ctgttcgacg cgctcgatac ctggctggac tgcctgcata
    23881 tggcggcgct ggtgctggac ggcattcagg tgaaacgtcc gcgttgccag gaagcggcgc
    23941 agcagggtta cgccaacgct actgaactgg cggattatct ggtggcgaaa ggcgtaccgt
    24001 tccgcgaggc gcaccatatt gtgggtgaag cggtggtgga agccattcgt cagggcaaag
    24061 cgctggaaga tctgccgctc agtgagttgc agaaattcag ccaggtgatt ggcgaagatg
    24121 tctatccgat tctgtcgctg caatcgtgcc tcgacaagcg tgcagcaaaa ggcggtgtct
    24181 caccgcaaca ggtggcgcag gcgattgctt ttgcgcaggc gcggttgggg taatttgttt
    24241 atattgctcc cctgaaaatg gggagcttac cacgcctaca aagtgtgaca cttcgtaggc
    24301 ggataaggcg tttacgccgc atcaggcaga aatttgtcag cattctggtg accttaaatt
    24361 taacgcccaa ccagaaccat tatttgtcac attccttttt attcttgcgt cttctttacc
    24421 ttcactctcc ccataaaaat tatattttc
//
LOCUS       DAZFRG010000052        24233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFRG010000052 DAZFRG010000000
VERSION     DAZFRG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.052"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            199..270
                     /gene="ypdK"
                     /locus_tag="ACHKUC_001621"
     CDS             199..270
                     /gene="ypdK"
                     /locus_tag="ACHKUC_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO6617896.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(334..1572)
                     /gene="alaC"
                     /locus_tag="ACHKUC_001622"
     CDS             complement(334..1572)
                     /gene="alaC"
                     /locus_tag="ACHKUC_001622"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO6617897.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            1949..3646
                     /gene="ypdA"
                     /locus_tag="ACHKUC_001623"
     CDS             1949..3646
                     /gene="ypdA"
                     /locus_tag="ACHKUC_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO6617898.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            3661..4395
                     /gene="ypdB"
                     /locus_tag="ACHKUC_001624"
     CDS             3661..4395
                     /gene="ypdB"
                     /locus_tag="ACHKUC_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO6617899.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            4408..5265
                     /gene="ypdC"
                     /locus_tag="ACHKUC_001625"
     CDS             4408..5265
                     /gene="ypdC"
                     /locus_tag="ACHKUC_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6617900.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRIVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(5268..7763)
                     /gene="ptsP"
                     /locus_tag="ACHKUC_001626"
     CDS             complement(5268..7763)
                     /gene="ptsP"
                     /locus_tag="ACHKUC_001626"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6617901.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(7788..8825)
                     /gene="ypdE"
                     /locus_tag="ACHKUC_001627"
     CDS             complement(7788..8825)
                     /gene="ypdE"
                     /locus_tag="ACHKUC_001627"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6617902.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(8825..9910)
                     /gene="ypdF"
                     /locus_tag="ACHKUC_001628"
     CDS             complement(8825..9910)
                     /gene="ypdF"
                     /locus_tag="ACHKUC_001628"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6617903.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQFELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRRQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(9925..11172)
                     /gene="fryC"
                     /locus_tag="ACHKUC_001629"
     CDS             complement(9925..11172)
                     /gene="fryC"
                     /locus_tag="ACHKUC_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6617904.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(11194..11520)
                     /gene="fryB"
                     /locus_tag="ACHKUC_001630"
     CDS             complement(11194..11520)
                     /gene="fryB"
                     /locus_tag="ACHKUC_001630"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6617905.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(11739..12704)
                     /gene="glk"
                     /locus_tag="ACHKUC_001631"
     CDS             complement(11739..12704)
                     /gene="glk"
                     /locus_tag="ACHKUC_001631"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO6617906.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            12908..14164
                     /gene="yfeO"
                     /locus_tag="ACHKUC_001632"
     CDS             12908..14164
                     /gene="yfeO"
                     /locus_tag="ACHKUC_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO6617907.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAITIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            14279..14605
                     /gene="ypeC"
                     /locus_tag="ACHKUC_001633"
     CDS             14279..14605
                     /gene="ypeC"
                     /locus_tag="ACHKUC_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO6617908.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(14746..15984)
                     /gene="mntH"
                     /locus_tag="ACHKUC_001634"
     CDS             complement(14746..15984)
                     /gene="mntH"
                     /locus_tag="ACHKUC_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6617909.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            16320..17522
                     /gene="nupC"
                     /locus_tag="ACHKUC_001635"
     CDS             16320..17522
                     /gene="nupC"
                     /locus_tag="ACHKUC_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO6617910.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(17572..19761)
                     /gene="pdeA"
                     /locus_tag="ACHKUC_001636"
     CDS             complement(17572..19761)
                     /gene="pdeA"
                     /locus_tag="ACHKUC_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6617911.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSSHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKIRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLIMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(19968..20043)
                     /locus_tag="ACHKUC_001637"
     tRNA            complement(19968..20043)
                     /locus_tag="ACHKUC_001637"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20008..20010),aa:Ala,seq:ggc)
     gene            complement(20083..20158)
                     /locus_tag="ACHKUC_001638"
     tRNA            complement(20083..20158)
                     /locus_tag="ACHKUC_001638"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20123..20125),aa:Ala,seq:ggc)
     gene            20379..20738
                     /gene="yfeC"
                     /locus_tag="ACHKUC_001639"
     CDS             20379..20738
                     /gene="yfeC"
                     /locus_tag="ACHKUC_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6617912.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            20740..21132
                     /gene="yfeD"
                     /locus_tag="ACHKUC_001640"
     CDS             20740..21132
                     /gene="yfeD"
                     /locus_tag="ACHKUC_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6617913.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(21183..22598)
                     /gene="gltX"
                     /locus_tag="ACHKUC_001641"
     CDS             complement(21183..22598)
                     /gene="gltX"
                     /locus_tag="ACHKUC_001641"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO6617914.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22857..22932
                     /locus_tag="ACHKUC_001642"
     tRNA            22857..22932
                     /locus_tag="ACHKUC_001642"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22890..22892,aa:Val,seq:tac)
     gene            22977..23052
                     /locus_tag="ACHKUC_001643"
     tRNA            22977..23052
                     /locus_tag="ACHKUC_001643"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23010..23012,aa:Val,seq:tac)
     gene            23099..23174
                     /locus_tag="ACHKUC_001644"
     tRNA            23099..23174
                     /locus_tag="ACHKUC_001644"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23132..23134,aa:Val,seq:tac)
     gene            23179..23254
                     /locus_tag="ACHKUC_001645"
     tRNA            23179..23254
                     /locus_tag="ACHKUC_001645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23212..23214,aa:Lys,seq:ttt)
     gene            complement(23365..>24233)
                     /gene="xapR"
                     /locus_tag="ACHKUC_001646"
     CDS             complement(23365..>24233)
                     /gene="xapR"
                     /locus_tag="ACHKUC_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO6617915.1"
                     /translation="TDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLG
                     TQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVVGTAM
                     WGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGFTSLR
                     LHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVV
                     IREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVYETQQVT
                     PALVKLLAALAQ"
BASE COUNT         6003 a         6478 c         5968 g         5784 t
ORIGIN      
        1 aaataagaaa atgcctcttg tctcgtttca gttttaggcg tacattagcg ccgtctcgag
       61 cagtttagct cagaattctg ggatgatgcc cacggtaaaa tacaggactt ttaaggtcgt
      121 tttgcccggt gtcaactcac tgtatcaggt gtaatgaagt cattcaggcg taacagtaat
      181 tacgcggaga gatgtaaagt gaaatatttc tttatgggca tttcttttat ggtcatcgtt
      241 tgggccggta cttttgccct gatgatctaa aagcaaactg tgcaatgaaa aacaggagcc
      301 atcaggctcc tgtttgcatt tctggcgaag gcgttattcc gcgttttcgt gaatatgttt
      361 gctgctggca ggtagtaaac cgtcggcacg gaacatcgct ttaatcccac gaatcgcctg
      421 acgaatacgg tcgcggtttt caatcagtgc aaagcgcaca tgcgtgtcgc cgtagtcgcc
      481 aaagccaatc cccggagaga cacagacctt cgcttcgtta agcaatttct tggcaaattc
      541 cagcgagccc atggcagcat acggttccgg gattttcgcc cagacataca tcgaagcctt
      601 cggcatttcg accatccagc ccgcttcatg cagccctttc accagcacat cacggcggcg
      661 tttgtactgt tcggcaatat cacgcacgca ctgttgatcg ccctccagcg ccgcaatagc
      721 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gtgccaacgc
      781 gctgaccagc gttttgttac cgaccataaa gccgatacgc cagcccgcca tgttgtagct
      841 tttcgacagc gtaaagaact cgaccgccac gtcgcgtgca cctggcacct gcatgattga
      901 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
      961 atcgtagcgt ttagccagcg ccacaacctt ctcaaagaat tccagttcca cgcattgcgc
     1021 ggttgggttt gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
     1081 ggcgcgctcc agttcattga agaagtcgac accttccacc agcggcactg agcgtacctg
     1141 cgccccggca atcaccgcgc cgtaaatatg aatggggtag cttggattcg gcaccagcac
     1201 tgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
     1261 agtgacgatg gcttctgatt ctgggtcgat ttcaacgtcg tagcgatcct gataccagcg
     1321 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgacgtg gagtaaccgt gcgtgtccgg
     1381 gcgctgggcc accgtacata atttttcgac gatgtgcggc ggagtcgcac cgtccgggtt
     1441 acccatactg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
     1501 ggtaatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
     1561 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
     1621 cacgatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
     1681 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttatctacg
     1741 ctaacaagaa aattaccact cattttatta acattataat ttcaataaat ttaccaacca
     1801 gatgtgcatt atcaagagat gcattgctaa tacaccattt atctttcaat ccccttttaa
     1861 aattgtggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
     1921 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
     1981 cttcgatcgg gccgcgttaa tgcttatctg cctgtttttt ctcatccgta tccgcctgtt
     2041 tcgcgaactg ttgcacaagt cggcacactc accgaaagag ctgctcgccg ttaccgccat
     2101 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
     2161 gaacgtacgt attatcgcgg tgatgtcagg cggaattctg tttggcccgt gggtgggcat
     2221 cattaccggc gtgattgcgg gtattcaccg ttatttaatt gacatcggcg gcgtaacggc
     2281 gatcccctgc tttatcacca gcattctggc gggttgtata tcgggctgga tcaacctgaa
     2341 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgaaacgtt
     2401 gaccatgatt ctggtgattg tctgggcacc aactaccgca ttggggatcg atatcgtctc
     2461 taaaatcggg attccaatga tcctcggcag cgtctgtatc ggctttattg tgttgctggt
     2521 acaaagtgtt gagggagaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
     2581 tatcgccaac aaaacgctgc cgctgtttcg ccatgtcaat agcgagtcat tacgcaaagt
     2641 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgatgacta ataccgatca
     2701 tgtgctggcc tacgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
     2761 cccgactacc cgccaggcga tgaattatgg aaaaatcatc attaaaaaca atgatgaagc
     2821 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
     2881 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
     2941 aatggcggtc ggtctgtcac aaatcatctc cacgcaactg gaggtgtcac gcgccgagca
     3001 gctgcgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
     3061 tttcctgttt aacgctttga acgctatttc atcgtcaatc cgcctgaatc cggatactgc
     3121 tcgccagctg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagatga
     3181 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
     3241 ggcccgcttt ggcgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
     3301 cattcccagc ctgctgatcc agccgttggt ggaaaacgcc attgtccacg gtatccagcc
     3361 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggtaatc gggtacgtat
     3421 tgcggtgcga gataccgggc acggcatcga tccaaaggtg attgagcggg tcgaagcgaa
     3481 cgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta
     3541 tggcgagggg ctgcatatcc gccgcctgga accaggaacg gaaattgcgt tttacattcc
     3601 taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt
     3661 gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt
     3721 aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag
     3781 tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat
     3841 ggtgtgttgc tggcacaaaa catcagccag ttcgcccata aaccgtttat tgtgttcatc
     3901 accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aagcgtttga ctacattctc
     3961 aaaccgtatc aggagtcacg cattaccggg atgctgcaaa aactggaagc ggcctggcaa
     4021 caacagcaga ccagcagtac gcctgccgcg acggtaacgc gtgagaatga caccattaat
     4081 ctggtgaaag atgagcgaat catcgtcacg cccattaacg atatctatta cgccgaagcg
     4141 catgagaaaa tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt
     4201 accgagtttt gcagcaagct gccgccgtcg cattttttcc gctgccatcg ctcattttgt
     4261 gtcaatctga acaaaatccg cgagatcgaa ccgtggttta ataacaccta tattctgcgg
     4321 ctgaaagatc tggattttga agtgccggtc agccgcagca aagttaaaga attccgccag
     4381 ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa
     4441 tttttcgccg atctgttcag cggcctggtg cttaacccgc aactactggg gcgcgtctgg
     4501 tttgccagcc agcctacctc attgccagtg ggcagtttat gtattgattt tccccgtctg
     4561 gatatcgtgc tacgcggcga atacggcaat ctgctggaag caaagcagca acgtatagtg
     4621 gaaggagaaa tgctgtttat tccggcacgc gcggctaatt taccggtcaa caacaaaccg
     4681 gtgatgctgt taagcctggt gttcgcaccg acctggcttg gcttatcatt ttacgatagc
     4741 cgcaccacgt cgttgttaca tcctgctcgc cagatccaac ttcccagcct gcaacgcggc
     4801 gaaggtgaag cgatgcttac cgccctcacc catcttagcc gttcgccgct ggagcagaat
     4861 atcattcagc cactggtgtt aagtttgctg catctttgcc gtaacgtggt gaatatgccg
     4921 ccgggcaatt cgcagccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag
     4981 gataattatg cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc
     5041 aatcatctgt caaaactgtt tgctcagcat ggaacaatgc gttttatcga gtatgttcgc
     5101 tgggtacgaa tggcaaaagc gaggatgatt ttgcaaaaat atcatctgtc gattcatgaa
     5161 gtggcgcagc gttgcgggtt tccggatagc gactattttt gtcgcgtttt ccggcgtcag
     5221 tttggtctga cgccgggaga gtatagcgca cgttttcagg gctaacgtca gaaggttaat
     5281 tccgtttcca gcagcgtcag aatactttgt gcatcttgcg cggcaaacag cgactggcgg
     5341 aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct
     5401 tcgttagcgc ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag
     5461 tcaaccggtt tcaccaaccg ggcaatgctg atactggaat gacggatcca ctgtgatttg
     5521 gtgtgcggga tcgccacgcc aaaaccaacg ccagtagtaa caatctcttc ccgctgccag
     5581 acatcttctt ccagctcgaa cggatgttca gtacgcccgt taacaccgag gttaccgcac
     5641 aggaactgga tcgcctgctc tttattacta aaggactgat caacaaagat attctctagt
     5701 gccagcagtg ggcgaacgtc ttcttccggc gcaaacgcgg taagtaacgc ttcaatttcc
     5761 tgcgcgctgc ggcactcgca cgcctgacgc gccagttccc gacacgcctc gctatccagt
     5821 tgacgaagct ggcttttcac tgccggaata cgcgggctac tcatgcttag ctcatccagg
     5881 cccagcccta gcagtagcgg cagataacga ctttcaccgc ccagttcacc gcaaatacct
     5941 acccatttgc cgcgctgatg cgcagcggta acaatctgct gcaacatgcg caggaacgat
     6001 ggcgtaatcg ggttatacag cggcgatacg cgcgggttat tacgatcgac tgcatacaga
     6061 tactgggtca tatcgttaga accgatactg aagaaatcga cctcatcgca gaagtggtcg
     6121 atgatgtagc acaccgacgg cacttccacc atgataccca gcgtaatcgt ctctgcatga
     6181 cgcaggccat cgcgcttaag ctcaacgatc gctttttgga gctcaccctt cacccataag
     6241 atctgatcga gactgtggac cattgggatc atcaactggg cgttgccgaa actggcagcg
     6301 cgcaaaatcg cccgcaattg agtacggaac aggccagcaa attccgggta aatacgtacc
     6361 gcgcgatagc cgaggaacgg gttctcttcc tggggaatgt tgaggtaagg aatgctttta
     6421 tcgccaccga tgtccatcgt gcggaagata atcggcttgt cgcccgccgc cagtagcacc
     6481 tgctggtacg cttcaaactg ttcctgctcg tccggcgcgc tgtcgcgatc catatagagc
     6541 atttcggtac gaaacagccc aacgccttcc gcgccgttag caaacgcgcc tggggcttcc
     6601 agagcggtgc caatattcgc cgcaatatca atacgtttgt tatcacggga ataggccagc
     6661 tgcgcggcag cttgcgcctg ttgtttttgg cgtttatccg ccagcgtctg cgccacctgg
     6721 taataaccgc tcaccgcatc gttgggatta attgccagta cgccgcactg ggcatcaagc
     6781 actgcaggtt gcccggcata acgggcaatc gcatccagcg gcaggccgct cagtaccggg
     6841 atcgccgaag cgcgggccag aatcagggta tgcgaggtgc gcccggtttt ctccagaatc
     6901 atgcccgcaa gattttttaa atcgaggctc aaaaactggc ttggggttaa atcttcagcc
     6961 accagaatgg tcggtttttc aagcaccaga ttattgcgcg gttttagttc cggccaggtg
     7021 atatgcagca actgttcgct gatatcgcga atgtcgctga cccgctcgcg cagataatcg
     7081 ctggcagaag cagaaagttt ggcacaaatc tgctccatat tgctgatgat cgccgccccc
     7141 agcccctgat gctgttctgc catcaggtga cggatattgc ctgcaaattc atcatcctga
     7201 atcagcgaca aatgggcgct gaggatagtt ttgctttcgc cgtcacgctc acgcaattgc
     7261 tggttcagtt gctcggcaag cgttgccaga ctgtgctcca gtagggtgga atcttgcgca
     7321 ctcgcaggga tcacccgata actgtcgagg ctgtcgctct gtaacagggt cagtgtaccc
     7381 acgccaacgc cgcttgccag cacgttgccg tacagtaaat ccgggttcag gcggcttaat
     7441 gaacgcggca gcggatgcgc cgtcagttct gccagcgtcg gctggacgct atcgctgtcg
     7501 ataaagcgca cctggatata ctcttccagc actcgccgcg cctgctcttc atcgctaccg
     7561 ctaatgttca ggctgcaact gtcattaaat aaagtgccag tgccaatcag cgccagcgag
     7621 cttttcgcat cggcctttgc gttctggcga tggttaataa aagtgatttc gctttgccac
     7681 tggctgcact gctctttaag ttcccacgcc ggacgagcat gtagaccgtt aggcaaagga
     7741 cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca tctaaaatcc
     7801 gtcagttgaa caaccgtctc acgcgtaaga cgttgaataa gggcagataa aagttgctcc
     7861 atctgcaaaa tgtcacggca gtcggcaatc gatgcggcgc aatgtccgtg gcgggttgca
     7921 ggccccatca ccactgtggg tacgccagtg ccggttaaat gcaccgcccc gccgtccgtt
     7981 ccgccgttgc tgaacatatc tgactgcaac ggtacgccaa tttctgccgc cacggtttcg
     8041 atccacgcgg taagttttgg cggcgcaatc agcgacttgt cgcttaacac cagcatcggg
     8101 ccgttaccaa tctggcgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg
     8161 gtatcaagca caatggcgac atccggcgac accgcgcggg tggcagtttg cccgccgcgt
     8221 aagccaacct cttcgctgga acttgccacc agccacactt ccgcaggcag ctcggcgtcg
     8281 tgcagctcac gcagtaacgt caccagcaga tagcaaccga ggcggtcatc aaaggctttc
     8341 cccatcaccc gctggtgagg gagaacctga aaagtggtat cgaaggtgac gcgatcgcct
     8401 ggacgaattc ctgcctgcat cacttcgtca caggagcgcg cgccaatgtc cacgcgcatg
     8461 gcgctgacgt cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca
     8521 cgggtagtga tacgcaccga ctgaagctgg cgggcagcca tgcgtacatt gccaaccggc
     8581 aacacatcga tcgccccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata
     8641 tgcgcacaga tcatcacctt cggacctgtc gactcattga gtcggatcag caccgatccc
     8701 agcccatcaa atcgcacttc tttttgcagg cgatccgctt cttcccgcag gatctgccgt
     8761 acttcctgtt ctgaggaggc gatcgcatct gcctcgctca acgcttttaa gagcgataaa
     8821 tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc
     8881 cttgcggggt gaccagcacg acatcttcaa tgcgcacgcc cccttgtcct ggcaaataaa
     8941 tccctggctc cacggtcagt aacatacctg gctgtagcgt cgtagtgtcc cgcggtgaaa
     9001 aacgcggttc ttcatggact tcaataccga tagcatgacc ggtgttatgg ccgaaaaact
     9061 tgccataacc ggcctcggta atcacgcggc gcgcggcatc gtcaacctgc tggcagcgca
     9121 cgccggggcg aattacagag attgccgcga gctgtgcctg caggacaatc tgatagacgt
     9181 taaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca
     9241 tatcagagca gtagccctga tacagcgcac cgaaatcgag agtgacaaac tcgcccgctg
     9301 caacaatctt gtcgctggct ttaccgtgcg gcagcgcccc acgccagcca ctggcgacaa
     9361 tggtatcgaa agaggctttt tctgcgccct gccggcgcat aaaccactcc agttcagcgg
     9421 ctatctcgca ttcgctcatc cccgcctgaa taaagcggcg aatatgctct gcgccacgat
     9481 cggcaatccc gcaggcaagg cggattttct ccacctcttc tggcgttttg atttgccgca
     9541 gcacatccgg cgtggcgctc accagtttcg cattgagttc gaactgccag cggtgcgcgg
     9601 tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga
     9661 tttgtctgac gatggtggca agcgtgtgca tcgcgtcgag caaatgcagc tggtaacctt
     9721 gcgtgcgggc ttctacatcc gcgtaatagc gcgaatccac cagaatgtgc gcactttcac
     9781 ggctaatcac cacataacct gatccggtgg agatccccag atgcggctgt ttgttctgcc
     9841 gtgaggagag aagcaccgca tccagttgtt gcgccttaag ccagtcgcgc agcgaagcga
     9901 gtaatgtcat caggattgtc cttcttacag gctatcgatt aacaatttgc ctttacggaa
     9961 catcatcaga cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc
    10021 cgccatgtag acgccgaggt tagtgaccag cggccatgcc cagatagcgg actccggaaa
    10081 ccattgcact gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata
    10141 cgacggaatg gcggtgatag ggctttccag tgcaaacgga atcgcaccct cactgatccc
    10201 cataaacgcc aggaacatcg cggttttacc ctgcggataa agctgggcgt tgaacaagcg
    10261 tttgccagtt aagcgacgat cgataatggt cgccagaccc agaccaatcg gcggaataac
    10321 gatagcgata gaacgtgcag tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac
    10381 gaaacctgcc gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc
    10441 cattgcgtac atcagcgcgc ccttctcacc tgcagcggtc agcacggtac ggataccgcc
    10501 gttgatccag ccaccaaagg gggtgatgac gtagtacatc gccagcatga caaaaatggc
    10561 ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat
    10621 tttctggttc atccacttca cgaggtaacc ggcaacgata gaaataatca gcgcaccaat
    10681 gaaggtggac ggcaccggcg aagaggtcgc ccattgcatc gtgctgggat cgaagttcag
    10741 cagttgcgtc ggctgggtag acatcaagcc gccgataaat ccagccggga acgccagttt
    10801 gccaccgata gagttcgcca caaaagcagc aaacatcgga atggcaaaac caaacaacac
    10861 accgccgaag gactgcgaca gccaggcaaa tttcagtaag gagaggtcga aaccggagaa
    10921 tttcccgcta ttaagagcat ccatgatgcc gatatcagca ggaattttaa gccagctata
    10981 agcaatcagc tgactgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg
    11041 tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctggatt tagaggcctg
    11101 cggattctta actgccgctg ccgcaccgga tgcgccatgc acaacggttg cactgcgttt
    11161 tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc
    11221 ttcgatttct ttgatgattc ctgcagcgtt tttaattgcg tcctgcaaag tgatttcata
    11281 aacgtcgcgt gattcgaaac gctcgttatc ttccggggta actgccacgg agtggatgat
    11341 gatggtcgct tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg
    11401 ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccagcg cctgagcggc
    11461 cataaaagtg tgagccaggc ccattgggca ggcacataag gcaatcagtt tcttactcat
    11521 cgttaattcc tcgttcagtg atagttgacg agtgaaatta tgaacgtgca aaacaaatcg
    11581 ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac
    11641 gcaggtcggc cttgtgaggg gacacaaaga aaaatttaat taactgtatg atttaaaaga
    11701 ttatcgggag agttacctcc cgatataaca ggaaggattt agagaatgtg acctaaggtc
    11761 tggcgtaaat gtgcaccgga accgagaaga cccggattgt catggacgat gagatatacc
    11821 ggaatatcat ggacatattc cttaaagcgc cctttatctt caaatgcggc gcggaaacca
    11881 gaggctttga agaactcaag aaagcgcggc acgataccac ccgcaataaa cacgccgcca
    11941 aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc
    12001 gacaacgcgc ggcggcaatc ggtgcagctg tcagccagcg cgcgttcggt aatatctttt
    12061 ggcttgagat tttctggcag gcggttgtcg gctttaacaa ttgcgcgata caaattcacc
    12121 agcccagggc cagaaagcac gcgttccgcc gaaacatgac caatttccgc acgcaatatt
    12181 tcgaggataa tgccctcttc ttcactattc ggcgcaaaat caacgtgacc gccttctcct
    12241 ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag ccctgttccg
    12301 gcaccgtaaa ccgcaatagg cttaccttct accggttctg cgccaccaaa ctgaatcaga
    12361 tgctcttttt tcagcatcgg gatcgccatc gatacagcag taaaatcgtt aataatttcc
    12421 agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg
    12481 gtcattgcca cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc
    12541 tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc
    12601 cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt
    12661 gcgttggtgc cgcccacatc accgactaat gcatactttg tcattcttca actgctccgc
    12721 taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg
    12781 aaaggcaggc tccctgtaaa tatcgatctg ggtcacacaa ttactttatc gtttcagcac
    12841 caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga
    12901 atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc
    12961 aattgggatt gcgtccagtc ttattctgat tatggtgatg aaaatcgcct cggtattaca
    13021 gaatttgctc tggcaacgac taccgggaac tctggggata gcccaggatt cacctctctg
    13081 gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca
    13141 gggtcatgcc ggaccagatc ccgcctgtga accgcttatc ggcgcaccgg ttccgccctc
    13201 tgcgctacca ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg
    13261 gccggaacat cctatcataa ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct
    13321 gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc
    13381 gctgtttggc acacctgttg cggcggcatt gatattttcg caaaccttaa atggcagtaa
    13441 tgaagttccg ctatgggatc gtctctttgc gccgttaatg gcggcagcag ctggtgcact
    13501 tactaccgga ttatttttcc atcctcattt ttcactgccc attgcgcatt acggacagat
    13561 ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attaccatcg cagcagggat
    13621 ggtcgccgta tggtgcttac cacggttgca cgcgatgatg catcaaatga aaaatccggt
    13681 gttcgtgctg ggtattggcg gatttattct cggtattctg ggggttattg gtggaccggt
    13741 ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac
    13801 cagcgattac tttttgctgg cggtgataaa acttgccgcc ctggtcgttg ctgccgccag
    13861 tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat
    13921 gcttcatgag cacgttcccg ccgttccagc ggcaataacc gtttcctgcg ctatactcgg
    13981 catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg cagtcgttgt
    14041 acccaatacc acattgctac cgctgctctg tatcgtcatg ctcccggcat ggctgttatt
    14101 agcaggtaag ccgatgatga tggtcaaccg tccgaagcaa cagccacccc acgataacgt
    14161 ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg gcgctttacc
    14221 tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat
    14281 gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg cggcaaacgc
    14341 aggtgaaatc accctactgc catcaatcaa attacaaatt ggcgatcgcg atcattacgg
    14401 taattactgg gacggtggtc actggcgcga ccgtgactac tggcaccgca attatgaatg
    14461 gcgtaaaaac cgctggtggc gtcatgataa tggctaccat cgtggctggg ataagcgtaa
    14521 agcgtatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gtggaaaagg
    14581 ccgcggacat ggacaccgcc attaaacgca tccgtcaatg gagcacaatg cctgatgcga
    14641 cgctagcgcg tcttatcagg cctacaaacc agctctttaa cgcgtagggc ggataaagca
    14701 tttacgccgc atccgccaat ggtgccaaat gcgacgctca ttcaactaca atcccagtgc
    14761 cgttcctacc agtaaccaga tattcagcgc cactaccagc accacaatca cccagcctgt
    14821 ctgttttacg cgtttactgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat
    14881 caacagtgga accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag
    14941 aatccgtgtc ggatctaatc ccatcagaat gacgataaat gacggcagca tggtgactgt
    15001 acgacgcacc cacagcggga tatgaaagcg aatgaagccc tgcatcacta cctgccccgc
    15061 cagcgtcccc accaccgttg aagacagtcc ggcagcaacc aggcttaatc caaagaccgt
    15121 tgccgcagca tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcggcaac
    15181 accagtatga ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac
    15241 aaaaccggca atcgtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg
    15301 acgcgaaccg ccatgtaaat gctgagtgag cgaagagtgc aaataaatca catgcggcat
    15361 aatcgtcgcc cctaacacgc ccgctgccag gaagaccgcc tccgaagtag gtaaactcgg
    15421 gatcaccatt cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat
    15481 gtaagccgcg gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc
    15541 gcgacgttgc agcattaaaa taaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa
    15601 caacgaaaca ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc
    15661 ggttgccatc gcaataattt ctgcctgaac ccaatagaac cacacgacgg gacgcgggta
    15721 gtgatcgcga atctgctccg ccagattttt accggtggca atccccagtt tggccgagag
    15781 gatttgaatc agcatcgcca tcaggttagc ccaaacgaca acccacaata actgatagcc
    15841 gaagctggca cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc
    15901 cgcaatgaac gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct
    15961 actctcaacg cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttta
    16021 tgctacgtta aatgagaatg attatcaaat tcatttaaat gggttgtgat gatttctctg
    16081 atagaccagg attatgacta cgaaaagata gatgactcga atgttatggt tatgtttaat
    16141 attagcacat ttacataacc ttccgcttcc atacacaaca tagcagaaat gtatgacaga
    16201 tcactatttt tgaagcttgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc
    16261 ttaaccatgt tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaagta
    16321 tggaccgcgt ccttcatttt gtactggcac ttgccgttgt tgcgattctc gcactgctgg
    16381 taagcagcga ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag
    16441 tgttactggc gtggttcttc ctgaactccg acgttggtct gggcttcgtg aaaggcttct
    16501 ccgaaatgtt cgaaaaactg ctcggttttg ccaacgaagg gactaacttc gtctttggta
    16561 gcatgaatga tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta
    16621 tctctgcgct gatcggtatt ctccagcata ttcgcgtatt gccggtgatt atccgcgcaa
    16681 ttggtttcct gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca
    16741 gctccctgat tctgggtcag tctgaaaact ttattgccta taaagatatc ctcggcaaaa
    16801 tctcccgcaa tcgtatgtac accatggcag caacggcgat gtccaccgtg tcgatgtcca
    16861 tcgttggtgc atacatgacc atgctggagc cgaaatacgt cgttgcggcg ctggtactga
    16921 acatgttcag cacctttatc gtgctgtcgc tgatcaaccc ttaccgtgtt gatgccagtg
    16981 aagaaaatat tcagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg
    17041 aatacattct ggcaggtttc aaggttgcca ttatcgttgc cgcgatgctg atcggcttta
    17101 tcgccctgat cgccgcgctg aacgcactgt ttgccaccgt gactggctgg tttggctaca
    17161 gcatctcctt ccagggcatc ctgggctaca tcttctatcc gattgcatgg gtgatgggtg
    17221 ttccttccag tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg
    17281 agttcgttgc gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgcggaag
    17341 gcatcatctc tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcgg
    17401 gtgcggttaa aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac
    17461 tggtttacgg ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt
    17521 aagaccatac ataaaaaagc cggggataat tccccggctt ttttacgcct gttaatcagc
    17581 taatggctgc ggacgaccaa ttaaatatcc ttgcagatat tgcaccccaa gtttatgcaa
    17641 tagcgcctgc tgctgtggcg tctcgacaaa ctccgcgacc acactcaatg acttcgcttt
    17701 cgccagatcg gtaattgatc tcacaatcat cgcatccagc gtgttcgtga cgatatcttt
    17761 cacaaagacg ccatcaattt tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt
    17821 ggcatatccg gtgccaaagt catcaatcgc aatccggaaa ccaaacttat gcagctgctc
    17881 gatgttgtac atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat
    17941 gaccgcctgc ggggagatgt aataacgctt aaacagacga ataatccgcc cggcaatatt
    18001 cttttgcagc agcgtgagcg gcattaaatt gactgaaaag cgtggacctt ttttgtcgca
    18061 agggtgtgtt gccagccact tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct
    18121 aaggttaaac tgagcaataa ggggcagaaa cttatccggg gtcataatgc cgccgtcata
    18181 cttcagtcgc gcgaggatct catcataacc ttcaccctct ttgttgcgaa ttggctgggc
    18241 gtagagcagc aaatcaccct gatctaacgc cgtgcgaatg gtattcagca atagcacctg
    18301 tttagtggtt tgcccggaaa ccatctcctc tctgctatcc agcgccagca catgatgatg
    18361 tgcgcaggat tgctccgcca gccagcttaa ctgccccaac aagggttgca gggtttcctg
    18421 atttccatca aaacgccccc aggcagcacc atagcccata tccagcccgg tattgttcca
    18481 gtgaatttgc cgactattca ggatgttaac catatgctgg agtcgccctt ccgtttccgg
    18541 cccgctcagc actaacagca gttcactacc cggcaattga tacaactttt cgttttcctg
    18601 catcaacggc agcagcgtac ggtaaattga gcggatacag tgaacgcgca ttattaagcc
    18661 gtaatgacga ctcataaact caagattatc aatgcgcagg cagcaaaaac tcttgcccgc
    18721 ctcttgctcc ggcgcttgct ccaacgcacg aaagttgggt agaagcgtta acggatcggt
    18781 cagcgcctgc aaatgccagc ggcggttaag ccattcacta cgatgataaa tacgcaccat
    18841 atagagcagg caaacactaa aggaaatcag tactgcgaga ataaatgcca gcgaatattc
    18901 ggttagtacc ccttgcaaaa agttctggtt gtaattcaga aggcaaagcg ttgaaacagc
    18961 ccaggtgaga tttaaaaacg gatagcgaat cttaccgaca ccgagggtaa agatgataaa
    19021 gaaaacaggt accaggtaac cggcaataaa gtcattttca tacggtgtgc acatcagaag
    19081 tagcagcacg ctaagagctg ccagccagct taaggtaaat gcgcgtttct ctttgctcaa
    19141 cgacggagcg atatccctac gccacaatat ctgtgcaaag tgggaactta caatcatgcg
    19201 ggtgagatag taaaaaagca tgttgtaaat cagcactgcg gtgaaaaggc ttagcaaatc
    19261 aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggta gatatcttta gcggaaaatc
    19321 aaagaaattt cccacaagat acatgctgca tttgatgcca atcggcgcca ccaaaccaag
    19381 ccagaacaga cgttgccaga cgtatcggct ggtcaatcca taacgccaac gcgtccccaa
    19441 ttgccagcgt aatattgcac aggcacacag gactgcaaac gcctggctgc cgatcatgac
    19501 ggcggtctgt aaaaaagaga gatgcaggtt ccacagattg gtgcaaaaca tcccgcataa
    19561 aatgggaacg acaccacgcc agccaaagat aaacaagatt gacagcatta cgcacagtgg
    19621 catccacgcc agaaagatat aattggaatg gataacagca agtggcgaaa ttaaacggga
    19681 tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga tattttttat
    19741 caggttatgc tccacaaaca tgagtacact actctcactg atcggggttt tcaggcacac
    19801 aaataataag tttattcgca tcgcgaagca aacaggggcc agcctgttgc tgttttttca
    19861 gtcagtgatg cattcaccac acggcactgc caggatatag actgtattac aaaagcggca
    19921 aaaggcagag acaaaaaacc cccgctttgc agcgagggtt tgaaatttgg tggagctaag
    19981 cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc
    20041 cccacgagtc gtttacgtac caaatttact gggtgcaaaa tttggtggag ctaagcgggt
    20101 tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccat
    20161 cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga atgtgtcaac
    20221 ggcaaattgc cacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac aattttggaa
    20281 aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt gttataaaat
    20341 gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag aggatgacgc
    20401 cagatgaact tgccagactg accggctata gccgccagac cattaataaa tgggtacgca
    20461 aggaaggctg gacaacgtca ccaaaaccag gcgtccaggg tggcaaagct cgactggttc
    20521 acgtcaatga acaggttcgt gaatatattc gcaatgctga acgtccagaa ggtcagggag
    20581 aagcgccagc cctttctggt gatgcgccgc ttgaagtgtt gctggtgaca ctggcgaaag
    20641 agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa gggattatcg
    20701 gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt acgcaataaa
    20761 atgaccaccg aagaactggc tgaatgcctc ggcgtggcta aacaaactgt taaccgttgg
    20821 atcagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg tcgtgccaga
    20881 cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc cttccataac
    20941 acaccgatgc ttctggaagc cgaagaaccc cttgccgaat acgcaccggg tattcgcact
    21001 cctgcttatc ggcaaatcat cagcgcaata gacaacatga ctcacagtga gcaagaaaaa
    21061 gttgcgcaat ttttgtcacg cgaaggaatt cgtaattttc ttgctcgtct cgacatagat
    21121 gaatcagcat aaataaaaaa cggcaggggt atctctcctg ccgtttatct tttatgcgct
    21181 aattactgct gattttcgcg ttcagcaata aaagccagcg ctttgttgat acgctcgata
    21241 ctgcgggtct taccgattgc gtgaacggta acatccagcg ctggagactg ccccgcaccg
    21301 gtaacggcga cacgcagcgg cataccaact ttacccatac ccacttccag ctcatccgcc
    21361 gtcgcctgaa tagcgtgatg aacgttttca gcggtccagt cagtaatcgc agtcagtttg
    21421 tcacgaacca cttccagcgg ctgacgcgct accggacgca gatgtttttt cgcggcgtcg
    21481 gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc tctgtgccat ctctttcagc
    21541 gtcttgcagc gttcgcccag cagtttcacc agatcagcca gctgcgggcc gttacgggta
    21601 tcgatatttt cctgctcaat gtgccactgt aagtgagtag caacatactc cggcggcaac
    21661 gcgttaatgt agtgatggtt cagccacagc agcttgtcgg tgttgaacgc actggcagat
    21721 ttgctgacgg cattcagagt gaagtattta atcatctctt cacgagtgaa gatttcctga
    21781 tcgccgtggg accagcccag acgcaccaga tagttcagca gtgcttctgg cagataacca
    21841 tcatcacgat actgcattac gctgaccgcc ccgtgacgtt tggacagttt tttaccgtca
    21901 tcgccgttga tcatagaaac gtgcgcgtaa accggtaccg gcgctttcag agccttaaga
    21961 atgttaatct ggcgcggtgt gttgttgata tggtcttcgc cacggataac gtgggtgatt
    22021 tccatatccc agtcatcgac aaccacacag aagttatagg ttggggaacc atcggtacgg
    22081 cggataatca gatcatccag ctcctggttg ctgaactcga tcggaccacg gatctgatcg
    22141 tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta cgacacacgg ctcatcatca
    22201 gcgtgatgct catgactgtg gcggcagcga ccgtcataac gcggcttctc acctttcgcc
    22261 atttgctctt cgcgcagcgc ttccaggcgc tctttagagc aatagcattt ataagctgtg
    22321 ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat caaaacgttt ggtctggtag
    22381 tacggacctt catcccactc caggctcagc cagttcatgc catccataat ggcttcgata
    22441 gcttccggcg tggaacgctc aagatcggtg tcttcaatac gcagcacgaa ctcaccgccg
    22501 tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg cgccgccaac gtgcagatag
    22561 cctgttgggc ttggcgcgaa gcgagttttg attttcatga aatggcctta cgtttagaaa
    22621 gatgccgaca accggcaaat cctggaaaaa ttgagtgggc gatattctat cactcccgcc
    22681 tgattcctca atgtagttcg ggttgcaacc ttacgcatta ttgttttcgt tgacaaattg
    22741 cgcaacacgg tctgttttgc gatcatcttg cttaaattta cgacgaacga acaatttctt
    22801 tagaaaatgc gttgactcat tttgaactct ccctataatg cgactccaca cagcgggggt
    22861 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    22921 catcacccac caactacttt atgtagtctc cgccgtgtag caagaaattg agaagtgggt
    22981 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    23041 catcacccac cactttctcg ccagctagat ttcttgtaac aatgtgaagt accgaagtgg
    23101 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
    23161 gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
    23221 ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct
    23281 tttttgctat ctgcaatttg cgaaattgcc tgatgcgctt cgcttatcag accaactatg
    23341 tccggcagtt cccgtgcctc tcatctactg cgccaacgcc gccagcagtt taaccaacgc
    23401 gggcgtcacc tgctgcgttt cataaacaat atataagtct gccgggatgc gttctttgag
    23461 cggacggaat atgacaccgg gccagttcat ctgcgcgtag ctgtccgcta tcaatgtgat
    23521 accaataccc atactgacca tggcgagtac cgtttgcggt tcattaactt cgcgaataac
    23581 aaccggtgaa aatcccacct gctggcaaac tcgctgcaaa aaatcccagt cagtgtacac
    23641 gggcggcatt gtaacgaaat actcgtcacg tagcgcttcc agcgggacgg tggaaaatga
    23701 tgagagatga tgctcttcag gcatcgccac cagaaacgcc gattcatgca accgtaagct
    23761 ggtaaaacca gtcggtggtt ctgtcgccat tcgccagatc ccggcatcaa gttcgcggcg
    23821 ttccagcaag gccatttgca tcgctggcat cttttcgcga aacagaactt caacgttagg
    23881 attttctcta aggaatcgcc gcataaccgg gcgcatccgt ccccacattg ccgttcccac
    23941 aaccccgagt tcaatccgcc ctgcttctcc ccgacctatt tgttcaaccc gagccaatac
    24001 attatttgca ttcaccagca atcgacgcga ttcttccatc aagattttgc ccgcgtgtgt
    24061 cagtacgacg ctgcgcgaat ggcgaataaa aagctgcgtg ccgagttgat tttccagctc
    24121 tttaatatga atactgagcg gaggctgaga catatttaaa cgcgctgctg cgcgaccaaa
    24181 atgcaactct tccgctacgg caagaaaata acggagcaac ttaagatctg ttc
//
LOCUS       DAZFRG010000053        24078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFRG010000053 DAZFRG010000000
VERSION     DAZFRG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.053"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..2375
                     /gene="pdeF"
                     /locus_tag="ACHKUC_001647"
     CDS             132..2375
                     /gene="pdeF"
                     /locus_tag="ACHKUC_001647"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO6617916.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2414..3955)
                     /gene="ppx"
                     /locus_tag="ACHKUC_001648"
     CDS             complement(2414..3955)
                     /gene="ppx"
                     /locus_tag="ACHKUC_001648"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO6617917.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFHGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3960..6026)
                     /gene="ppk1"
                     /locus_tag="ACHKUC_001649"
     CDS             complement(3960..6026)
                     /gene="ppk1"
                     /locus_tag="ACHKUC_001649"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO6617918.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYELVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6198..6836)
                     /gene="purN"
                     /locus_tag="ACHKUC_001650"
     CDS             complement(6198..6836)
                     /gene="purN"
                     /locus_tag="ACHKUC_001650"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO6617919.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6836..7873)
                     /gene="purM"
                     /locus_tag="ACHKUC_001651"
     CDS             complement(6836..7873)
                     /gene="purM"
                     /locus_tag="ACHKUC_001651"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO6617920.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8198..8824
                     /gene="upp"
                     /locus_tag="ACHKUC_001652"
     CDS             8198..8824
                     /gene="upp"
                     /locus_tag="ACHKUC_001652"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO6617921.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8910..10199
                     /gene="uraA"
                     /locus_tag="ACHKUC_001653"
     CDS             8910..10199
                     /gene="uraA"
                     /locus_tag="ACHKUC_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO6617922.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10294..10995
                     /gene="hda"
                     /locus_tag="ACHKUC_001654"
     CDS             10294..10995
                     /gene="hda"
                     /locus_tag="ACHKUC_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO6617923.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLCQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11133..11492)
                     /gene="arsC"
                     /locus_tag="ACHKUC_001655"
     CDS             complement(11133..11492)
                     /gene="arsC"
                     /locus_tag="ACHKUC_001655"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO6617924.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADISLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11513..12976)
                     /gene="bepA"
                     /locus_tag="ACHKUC_001656"
     CDS             complement(11513..12976)
                     /gene="bepA"
                     /locus_tag="ACHKUC_001656"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO6617925.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13189..14250
                     /gene="yfgO"
                     /locus_tag="ACHKUC_001657"
     CDS             13189..14250
                     /gene="yfgO"
                     /locus_tag="ACHKUC_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6617926.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14349..14819)
                     /gene="bcp"
                     /locus_tag="ACHKUC_001658"
     CDS             complement(14349..14819)
                     /gene="bcp"
                     /locus_tag="ACHKUC_001658"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO6617927.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14819..15391)
                     /gene="gcvR"
                     /locus_tag="ACHKUC_001659"
     CDS             complement(14819..15391)
                     /gene="gcvR"
                     /locus_tag="ACHKUC_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO6617928.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15537..16415
                     /gene="dapA"
                     /locus_tag="ACHKUC_001660"
     CDS             15537..16415
                     /gene="dapA"
                     /locus_tag="ACHKUC_001660"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO6617929.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16432..17466
                     /gene="bamC"
                     /locus_tag="ACHKUC_001661"
     CDS             16432..17466
                     /gene="bamC"
                     /locus_tag="ACHKUC_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO6617930.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWLQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17679..18392
                     /gene="purC"
                     /locus_tag="ACHKUC_001662"
     CDS             17679..18392
                     /gene="purC"
                     /locus_tag="ACHKUC_001662"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO6617931.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18560..19423
                     /gene="ypfJ"
                     /locus_tag="ACHKUC_001663"
     CDS             18560..19423
                     /gene="ypfJ"
                     /locus_tag="ACHKUC_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO6617932.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19438..21453
                     /gene="tmcA"
                     /locus_tag="ACHKUC_001664"
     CDS             19438..21453
                     /gene="tmcA"
                     /locus_tag="ACHKUC_001664"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO6617933.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARQGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGLRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21527..22225
                     /gene="ypfH"
                     /locus_tag="ACHKUC_001665"
     CDS             21527..22225
                     /gene="ypfH"
                     /locus_tag="ACHKUC_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6617934.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22306..22506)
                     /gene="ypfN"
                     /locus_tag="ACHKUC_001666"
     CDS             complement(22306..22506)
                     /gene="ypfN"
                     /locus_tag="ACHKUC_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO6617935.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22534..23661)
                     /gene="dapE"
                     /locus_tag="ACHKUC_001667"
     CDS             complement(22534..23661)
                     /gene="dapE"
                     /locus_tag="ACHKUC_001667"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO6617936.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDAARWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23665..24021)
                     /gene="yffB"
                     /locus_tag="ACHKUC_001668"
     CDS             complement(23665..24021)
                     /gene="yffB"
                     /locus_tag="ACHKUC_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO6617937.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5627 a         6003 c         6322 g         6126 t
ORIGIN      
        1 tcaattggaa ctttcatatg acacttagaa tcattgctct ctatagtgat gaataatcat
       61 cattcgaagt caggtgggat gcctgtctga atacacctcc ttcaggatgt gggggattct
      121 gctgagcatc tatgaaactg aatgcaactt atataaaaat acgtgataaa tggtgggggc
      181 ttccgctgtt cctgccttct ttaatcttgc ccattttcgc ccacattaat actttcgcgc
      241 atatttcttc cggtgaagtt tttctctttt atctgccact ggcactgatg atcagcatga
      301 tgatgttttt cagctgggcg gcattgccag ggatcgcctt agggattttt gtccgcaaat
      361 atgcagagct gggtttttac gaaacgctct cattaacggc taattttatt atcattatca
      421 ttctctgttg gggcggttac agggttttta ccccccggcg taacaacgtt tcacatggtg
      481 atagccgttt aatttcccag cgactattct ggcagattgt gtttcctgca acgctgtttc
      541 tgatactttt ccagtttgct gcgtttgtag ggttactggc gagcagagaa aatctggtcg
      601 gcgtcatgcc ctttaacctc gggaccttaa tcaattatca ggccttgctg gtgggtaatc
      661 tgattggtgt cccgctgtgc tacttcatca ttcgggtggt gcgaaatccg ttttatttac
      721 gtagctatta ttcgcaatta aaacagcagg ttgatgccaa agttaccaaa aaagagttcg
      781 caatctggct actggcatta ggtgctttac tattgctgtt atgcatgccg ttaaatgaaa
      841 aaagcacgat ttttagcacc aattacacct tgtcattatt gctgcccctg atgatgtggg
      901 gagcgatgcg ctatggttat aagctgattt cattgctctg ggcggtcgtg ttgatgatca
      961 gcatccacag ctatcaaaat tacattccca tttatcctgg ctataccacg cagctcacca
     1021 taacctcctc cagttatctg gtattctctt ttattgtcaa ttacatggct gtactggcaa
     1081 cccgtcagcg agcggtagtc agacgcattc agcggcttgc gtatgtggac ccggtggttc
     1141 atctgccaaa tgttcgcgcc ctgaatcgcg cgttacgtga tgccccctgg tctgcgcttt
     1201 gttatttacg catccctggc atggaaatgc tggttaagaa ttatggcatc atgctgcgga
     1261 ttcaatacaa gcaaaaactt tctcactggc tgtcaccatt gttggaaccg ggtgaagatg
     1321 tttatcagct ttcgggtaac gatctcgcgc tgcggctgaa tacagaatcg caccaggagc
     1381 gcattaccgc actggatagc catctcaagc aatttcgttt cttttgggat ggaatgccga
     1441 tgcaaccgca gattggcgtc agttactgct atgtgcgctc gccagtgaat catatctacc
     1501 tgctgctggg agagctaaat acagtggccg aactttccat cgtgaccaac gccccggaaa
     1561 atatgcagcg tcgcggagca atgtatttgc aacgcgaatt gaaagataaa gtcgcgatga
     1621 tgaatcggct acagcgggcg ctggaacaca accatttttt cctgatggcc cagccgatta
     1681 ccggtatgcg tggtgatgtc taccatgaaa ttcttctgcg catgaaaggt gagaatgatg
     1741 aactgatcag ccccgatagc ttcttaccgg tcgcgcacga atttggttta tcgtcgagta
     1801 tcgacatgtg ggtcattgag catacgctgc aatttatggc tgaaaacaga gcgaagatgc
     1861 ccgctcaccg ttttgctatt aatctgtctc caacctcggt atgtcaggct cgttttcctg
     1921 ttgaagtcag tcagctgctg gctaaatatc agattgaagc gtggcaactt atttttgaag
     1981 tcaccgaaag taatgctctg accaatgtta agcaggcgca aatcaccttg cagcatcttc
     2041 aggaattagg ctgccagatt gcgattgatg atttcggcac cggctacgcc agctatgcgc
     2101 ggcttaaaaa tgtgaatgcc gatctgctta aaattgacgg cagttttatc cgcaatattg
     2161 tgtcaaatag tctggattat cagatagtgg cgtcgatttg ccacctggcg cgaatgaaga
     2221 aaatgcgggt agtggcagag tacgttgaaa acgaagagat ccgcgaggcg gtgctctctt
     2281 tggggatcga ttatatgcag ggttatctta ttggtaagcc gcaaccgtta attgatacgc
     2341 tgaatgaaat cgaacccatt cgcgaaagtg cctgaataat gcgggccgac atttctcgtc
     2401 ggcccgcaaa gcattaagcg gcgatttctg gtgtactttc ttcttcaatt ttcaaccgcc
     2461 agccagccac gccttcccag tattcttgct ccttttccag atcaagcagt accagcgcat
     2521 tctgactaaa ccagtcatgc gggaaacgca gtgtccagtg actgtcatca gtaatcagcg
     2581 tcaatgttgg cggtgtggtg gttgcctgac gttgattatt gagtaatacg ccaaggcgca
     2641 atagctgaat cagtggcagg aactgtttct tcttaaacaa ggtaaagcgc ggcagatcgt
     2701 cgagtttaat cgctttacgg tgatagcgca ccagtgtcgc catcatcagc tgctgttcct
     2761 gattaaaacc cggcaagtcg ctgttttgca gaatataagc ggagtggcga tgcaaaccgc
     2821 tgtggttgat attcaacccg acctcatgca gcatggcggc ccatcgcagt agcgcctcca
     2881 gttgtggatg cgccagcttc ggttgctgtt cccgccactg ttcgtacatt tgcatagtgg
     2941 tatccagcac ccgtcgggcc tgttcgctgt cgatgtgata ctggttggcg aggctgctgg
     3001 cagtgcgact acgcacatcc tgatgacgga aacgtccttc catttcatac agtacgcctt
     3061 cgcgtaacgc cccgtcagaa aggcgcagtt cacggatggc taaagcatca aacacaccgc
     3121 ataaaatcgc cagtccagga acgaagactg ttttccgctc ttcggaaaga ccaggtaaac
     3181 tcagcgatgc gaaattacgg tgccgtaaaa cttcttttac cagtttttcc agacgttccg
     3241 gggtaattat cccgtctttc tcgcccattt ccattaacac ttcatgggcg gcttttatgg
     3301 taccggaagc gcccattgcc acgttccagc cctgaattcg aaattgccag gttaacgttt
     3361 ccagtttttg tgctgccgcc atgcgagcgc gctgaaaatt ctctttattg atgaccccgc
     3421 catgaaaata aagctgggca aagctgacac aacccatccg gcggctttca acgagaatcg
     3481 gttcgaaatt ttcaccaatt accagttctg tagaaccgcc gccaatatca ataaccagtt
     3541 tgcgaccttt ttccggttgg gtatgttcca cgcccataaa aatcagacgg gcttcttcat
     3601 taccggaaat aatttcgatc gggtagggaa tgactttttc cgcgcgtttc agaaagtcag
     3661 tggcgttcag cgcctgacgc agcgtatggg taccaactat acagacgctg gcaggagaaa
     3721 acccttgtag cctttcggca aatagcgaca gacagttcaa accgcgcgtc attgcctctt
     3781 cactcaacat attatctggc cccaggccgt ccgccagatg cacccgctgt ttcaggcggc
     3841 caataatctg catggcacca tctaccacac gggctatgac catgtgaaaa ctgtttgaac
     3901 caagatcgac cgcagcaaac tcctgcggac gaggggattt atcgtgtatt ggcatagggt
     3961 tattcagatt gttcgagtga tttgatgtag tcgtaaatcg ccaactgcgc ccgtactttg
     4021 cggcgattgc cgcggggaac gtagcgatta ctgagttctt tatcgatata acgtgctttg
     4081 accgtatcgc tgaacaatat gtcgatgatg tccagcaccc gctgcttcag gcgcggatcg
     4141 agcagcggcg ttgccacttc aatacgataa tcaatattgc gcgtcatcca gtcggcggaa
     4201 gaaaggtaga cctttttatc gccgccattt tcaaaaatat aaacccggtc atgttcaagg
     4261 taacggtcaa caatactgat ggcacgaatg ttgtcgctaa tgccttccag attggggatc
     4321 agagaacaca ttccgcgcac cagcagatta accggtacgc cggagctgga ggccgcatac
     4381 agacgatcaa ccaggccttt atcgacaagg ttatttagct tcagggtgat accactgggc
     4441 agcccttgct gcgcgttggc gatctcgcgg tccaccaatt catacaacag acggcgagag
     4501 ttttgcggcg acaccattaa ataatcaaat gtcaccggac ggtatgggtt ttcaataaag
     4561 ttgaacaccc gccgtacttc gttagtgatg cgcgcatcgg cggtcagcaa cgaatagtca
     4621 gtataaagac gcgcggtttt ttcgttaaag ttcccggtcc cgatgtgtgc gtaacgcacc
     4681 acttcaccgt tttctttacg tgaaatcagg aacagtttgg cgtgaatttt cagccccggc
     4741 gcagagaaga taacgtgcac gcctgcttcg gtcaggcgct tcgcccagtg aatgttggct
     4801 tcttcgtcga aacgcgcctg taactcaacc accacggtga ctttcttacc gttgtgtgcg
     4861 gcgtggatca tcgagtcgat gatgcgtgaa tctttcgcca cgcggtaaat gttaatttta
     4921 atcgccagta cgctcgggtc gaacgaagcc tgacgcagca gttccagtac atgctcaaag
     4981 gtgtgataag gataatagag caacacatcg cgttcgcgaa tggcatcaaa accattgcgg
     5041 aactgggctt tatcaaacca aatatggcgt aaacgcggca gtggtttgtt caccagattg
     5101 gctttgccga cattggggaa attaataaag tctttaaaat tatgataacg accgccgggg
     5161 acgatggagt cgtagcggga aatagtcagt ttttcgcgta acacttcaac cagagcattg
     5221 ggcatatcgc gctgataaac aaaacgcacc ggctcagcag ttaaacgctg cttgagactg
     5281 gaagacatca actccatcag actggcttcc atctcatgca ctaaatcgta ttcggcatcg
     5341 cgggtcatct tcattgaata ggcattcagt gcgtcataat caaagaagcc tttgaaaata
     5401 tcatcaaggc agtaacgcag aatgttatcc agaagaatca tcggcttgcg tcgacgcggc
     5461 gcttctggcg gtaaattcac aaagcgcggc actttatctg atgggatctc cagcagcgcg
     5521 taacggatgg tatcgccacg gataatttcc accgccagat aggtgtaatc atctttcagg
     5581 aactgcacta agtcagtgtc aggattgatt aaaatcggcg taatgtgctg acgcagatac
     5641 tgcttaaaat aatgacgcag ccagttttgt tgattgacgg agagctggcg ttcattaatc
     5701 aggaagatct ggttgcgcgc catttccagc agtagctcgt tgtagaggcc gtcgaattcc
     5761 tgatcggctt tcagcacacg ggactgaatt ttgcccagta aatggcggga atgagagtta
     5821 gagccttgtt cttcgctaat aatgatgcgt cgcttcagtt cagcgaagcg gactttatag
     5881 aactcatcaa ggttattgga atagatcccc aggaaacgca tcctttcaat cagcgggtta
     5941 gatttgtccg ccgcttcctg aagcacgcgt tcattgaacg ataaccaact gagctctttt
     6001 tcgatgtata gcttttcctg acccattacc acttttactc cgttttattc acgggacact
     6061 gcctggatat tatggcgagc aatattggca gatgtccaac tatgccagaa aagggggaaa
     6121 aaaactgctg gagcttgcga tttacaggtg aattgaaaaa cgccagcggc agagctggcg
     6181 ctttaactac gggggcatta ctcgtcggca gcgtagccct gcggcggcag acgttgacca
     6241 tccagccacg cggcgttttc gtgcattttc agacgaccat cggcaaacca gctaatcacc
     6301 agtggataaa tagcgtgttc ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca
     6361 ccagcaaata ccgggacttt cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg
     6421 acgaaatgca ccgatgtacc gtgctcctca tcgccatttt ccagcgcctg acgatgggtg
     6481 tgtaatccgg gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg cccggcatag
     6541 tgggagacaa acgccgggct gagaatgcgc ataaaaccag ccagcacgac cacatcgggt
     6601 gcgtacatgt cgatttcatg aatcaactcc cggtcatagg cttcacgact gtcaaacgcg
     6661 ctggtgatga gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg
     6721 tcggccttat tgctgaaaac tgcccgtacg gtgcctttaa ttttgttggt tttgcaggcg
     6781 tcaataattg cctgcaaatt acttccgttg ccggaaataa gcaccacaat attcattatt
     6841 cgataaccac gcgttgttcg gaatcagagg ctttgatgat accgattttc cacgcgtttt
     6901 caccgttggc attgagcagg gcgagggctt tgtccacttc cggagcaggc agggcaataa
     6961 tcataccgac gccgcagttg aaggtgcgat acatttcatg gcgctcaacg ttacctgccg
     7021 tttgcagcca gttaaacact tccggccact gccaggaaga ttcatcaatc actgcctggg
     7081 tattatctgg caatacgcgc ggaatgtttt cccagaaacc gccgccggtc aggtgcgcaa
     7141 tggcatgcac atcgaccttt tcaatcaact tcagcactga cttcacgtaa atgcgggtcg
     7201 gtgccagcag atgatcggct aatggcttac catcaagttc ggtggtttgc ggatcacaac
     7261 cgctgacttc aagaattttg cgcaccagcg aatagccatt cgagtgcggg ccgctggagc
     7321 cgagtgcaat cagcacatcg ccgtcgctga ctttagagcc gtcaatgatt tctgattttt
     7381 ctaccacgcc aacgcagaaa ccagcgacat cgtaatcttc accgtgatac atccccggca
     7441 tttccgccgt ttcgccaccc accagtgaac agcccgattg cagacaacct tccgcaatgc
     7501 cgctgatcac cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga
     7561 aaaacagcgg ttccgcacct tgcaccacca ggtcattaac gcacatggcg accagatcaa
     7621 taccaatggt gtcgtgacgt tttaagtcca tcgccagacg cagcttggta cctacgccgt
     7681 cagtgccgga aaccagcaca ggttcacgat atttttgcgg caatgcacac agcgcaccga
     7741 agccgcccag accgcccatc acttccggac gacgcgtttt cttcactacg cctttgattc
     7801 ttccaaccag agcattaccc gcgtcaatat caacaccggc atctttgtag ctaagagagg
     7861 ttttatcggt cactgcttgg gtccccacgc gttacttgcg gtagaaaaat aaaattcggc
     7921 gcaattctaa cagggaaagc aaacgtttgc gagactgctt tacacaacct ttttgcacgt
     7981 cttttcccca ggcgcgcggc gaaagaatac ttgtaccagg gcaaaggatc gttttcggat
     8041 ggaataatct tcttccataa ccatttgaat ataaaataac tttatctcaa accgttatca
     8101 ttttgactaa agtcaacgaa aataatattg ccgccttgaa gaaaggaggt ataatccgtc
     8161 gatttttttt gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca
     8221 cccactcgtc aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt
     8281 tcgcgaactc gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga
     8341 aacggaaaaa gtcactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg
     8401 taagaaaatt accgttgtgc caattctgcg tgcgggtctt gggatgatgg acggtgtgct
     8461 ggaaaacgtt ccgagtgcgc gcatcagtgt cgtcggtatg taccgtaatg aagaaacgct
     8521 ggagccggta ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat
     8581 cgttgaccca atgctggcaa ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa
     8641 agcgggctgc agcagcatca aagttctggt gctggtagct gcaccagaag gtatcgctgc
     8701 gctggaaaaa gcgcacccgg acgtcgaact gtatactgca tcgattgacc agggactgaa
     8761 cgagcacgga tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa
     8821 ataaagaata aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc
     8881 ctataacaca taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa
     8941 gaccgccact tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg
     9001 caaccgtcct ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg
     9061 gtataggaac actgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt
     9121 ccagctttgc ctttatttca ccggtattgt tactgttgcc gttggggtat gaagttgcgc
     9181 tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag
     9241 cggggaccgg ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg
     9301 tcatcggtct ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag
     9361 ggcaaacgcc agactccaaa accatcatca tctctattac caccctggcg gtcacggttt
     9421 taggttccgt gctgtttcgt ggtttcctgg caattatccc gattttaatt ggcgtgctgg
     9481 tggggtacgc gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc
     9541 actggtttgc gctgccgacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta
     9601 ttctgccagc ggcgttagtg gttattgccg aacacgtagg gcacctggtc gtaacggcta
     9661 atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg
     9721 gcttgtcgac cgtgatctcc ggtttctttg gttccacacc aaacaccact tacggagaaa
     9781 acattggcgt gatggcgatc acccgtgttt acagtacctg ggttatcggc ggtgcggcga
     9841 ttttcgctat cctgctttcc tgcgtcggta aactggctgc tgctatccag atgattccat
     9901 tgccggtgat gggcggcgtt tcgctgctgc tttacggggt catcggtgct tccggtattc
     9961 gtgtgttgat cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg
    10021 ttattttgat catcggcgtc agtggtgcga aggtaaacat tggcgcggcg gagctgaaag
    10081 gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt gatcttcaaa ctgattagta
    10141 tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat
    10201 gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc
    10261 tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg
    10321 ccactttatc ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct
    10381 ttactggccg cgctgcaaaa cgtgctgtgt caggaacata gcggttacat ctatctctgg
    10441 gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcacag
    10501 cgtggcgatg cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg
    10561 ctcgacggta tggagcattt gtcgctggtc tgtattgaca acattgagtg tattgccggc
    10621 gatgagttgt gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa
    10681 acacggttgt tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg attaccggat
    10741 ctcgcgtcgc gtctcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat
    10801 aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg
    10861 gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca cgttgtttat gacgctggat
    10921 cagctggatc gtgcgtcgat taccgcgcaa cgcaagctga ccattccttt tgtgaaagaa
    10981 attctgaagt tgtagataag gtgtttattg tcggatgcgg cgcgaacgcc ttatccgacc
    11041 tacgcgaagt taatccatag tattcgtagg ccggataaga cgcggcaagc gtcgcatccg
    11101 gcaatgtgct tcaactagcg atggagcaca tcttaaccca cgatttccag tacctgctcc
    11161 ggtggacgac caatccgtgc tttgccattc gcgaccacaa tcgggcgttc cattagcttc
    11221 ggattatcca ccatcgcctg aatcagcgcc tcttcgctaa gtgaaatgtc agccaggttc
    11281 agttctttat aaagatcctc tttttggcgc atcaattcac gagcgctatt catccccagc
    11341 attttcagca aatcacgcag cgttgccgca tcggcgggtg tctcaaggta gagcaccact
    11401 tctggctcca cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt
    11461 gggttatggt agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct
    11521 tggtataagg cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac
    11581 gcgcttgttg caggctgcct aatttcacct gagaactggc gctactcaac aaggaaatag
    11641 cctgatcgag tcgcccggcg agcgcataac cttctgctcg cgcagccagt tcctgatcgc
    11701 ggttatttag tgcggcttcc gcctgtgcca gtaaatccca gccgttgctg tcatctttat
    11761 tgttaaaagt gtagcgattc agaatattgg ccgcttcctg tggttgcccc ccttgcagat
    11821 aagcgttcgc caggttgagc tgcaacaccg gattggtgcg caaatcgcgg gcatttttca
    11881 ggcgattgat agcgtcattg gctttgtttt gcccaagatc gatatcggta gccagatcga
    11941 gataccatgc gttgccaggt tctgccgcca gtaacggttg cagcgtttta cgcgcttcgt
    12001 cgtatttatt ggcttccatc gcctgtaaag cacgaccata ttgcgccgct cgttgctgac
    12061 gaacgtttcc tttcgcccat tcatccagca aatcactggt gagttggtta cgccctgaat
    12121 tgtacatccc cagtgtgcgc acttttgcca gatagaaatc ttctgacgat tgcaccacta
    12181 tcgggcgcat ctgattggca cggttacggg catcagccag acggctttcc ggtagtgggt
    12241 gagtgagcag aatttctggc gggcgcgaag agtaacgcgc ctgatcgagt aatttttcga
    12301 ggaaggtcgg catcgcctgc ggatcgaatc ccgagcgttg cagcacctga ataccaatgc
    12361 ggtccgcttc ctgttcattt tgctgggtga aactgatcat cccctggcgc gttcccgcca
    12421 gtgtaccggt cagcgccgcc atccccgcct gcggactggc catcgccagt aaaatagaac
    12481 ctaacgcgcc gacccaggtc agcggcgcgc tgcgctgctg atcttccatc gctcgcgcca
    12541 ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac tgaagccagt tgactttcgt
    12601 tatcggaata acggaacagg gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg
    12661 cgttaatttc atcgttgttg atcagaaaga aatgaaacgg tgtcttaacc gaattggcat
    12721 gcgaaaccag acgcattcct agcgaattaa tgtattgcgt taacagcgga tcattaatta
    12781 acggcgcgct gccgcgtaac tggcggacat aatagtcgcc catctgcatt tcctgaccaa
    12841 tggaaagtgt gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa
    12901 acgccggggc tacctgacca atggtcatag cagcaatgag ggttgcaacc aggttttttt
    12961 tcaactgcct gaacataacc tctatcctgt atttctggag atagtcattt gaccgatact
    13021 gtgaaatatc gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gcccactcaa
    13081 atgacttttc agtttcctga atccgtcata aaaagcgaag tcttttttgt gacatttcga
    13141 tacaattgcg gatcgcaatc ccgctcttga ggttcaggga aggtctgtat gctcgaaatg
    13201 ttgatgcaat ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt
    13261 ttggttgctg gttttggcat tatctttttc tttagcggcc tactcgcgcc gttgctggtg
    13321 gctattgtgc tggcctattt actggaatgg ccgactgtcc gcctgcagtc tattggctgt
    13381 tcccgccctt gggcgacatc tatcgtgctg atcctgtttg tgggcatcct gctactgatg
    13441 gcgttcgtgg tactgcctat cgcctggcag cagggcatct acttaatccg cgatatgccg
    13501 gggatgctca ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgtta
    13561 atggatgcgg gcattattga tgcaatggct gaaaatatgc gcagtaggat gctgaccatg
    13621 ggcgattcgg tggtgaaaat ttccctcgcc tcgttggttg gtttgctgac cattgccgtc
    13681 tatctggtgc tggtgccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg
    13741 aacgccgttc gccgggtgct gccgcgcaac cgtggactgg caggacaggt gtggaaggag
    13801 atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtggtaggg
    13861 atcgccacct ggctggggtt cttgctcttt gggctgaact attcgctgct gctggcggtg
    13921 ctggtcggct tctcggtact tattccgtac attggcgcat ttgtggtgac cattccggtg
    13981 gttggcgtgg cgctattcca gtttggtgct ggcacggaat tctggagctg cttcgcggtg
    14041 tatctgatta ttcaggcgct ggacggcaac ctgttagtac cggtgttgtt ctccgaagcg
    14101 gttaacctgc atcctctggt gattatttta tcagtggtga tcttcggtgg tttgtgggga
    14161 ttctggggcg tattcttcgc tattccattg gcgacgctga tcaaagccgt gattcacgcc
    14221 tggcctgatg ggcaaatcgc gcaagactga ccttcagcga aaaaatttta aacgagtgtt
    14281 gcagggcagt gagaagattt gagatgtgct ggccgcaagc gcagccagca cggaatggag
    14341 caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg attgctggtt
    14401 ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa gctgatgcga
    14461 tgaataccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc gaattgttcg
    14521 cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt ttccgcaaaa
    14581 cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc cgcttttttc
    14641 aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg ggtcatggct
    14701 ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt aacttgttct
    14761 ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag tggattcatt
    14821 acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata ctgccttgtg
    14881 cattgagttc tgtacatagg gctttgaacg cttgctcaat atttgctgcg tccgcagatg
    14941 cggggctgtg ggcagttatc tgaatatgca actgcgcagc ccgttcattt tcagcaggtt
    15001 gcgtgcgcga caccagctcc gcaatgttca tatgatgcgc gtcgaaaagt gctgtgaagc
    15061 gttcaattaa atgcggggag tctgccacat cgacctgaac ccagacagat gctggcattg
    15121 gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagc tcggcacctt
    15181 tcaacggtaa cgttgattca atcagagtaa tggcattcca tgaaccggaa agcagcatga
    15241 taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta cagccgcaac
    15301 tactgacatg acgggtgatg gtgttcacta ttccagggcg atcggcaccc aacgcagtga
    15361 tcaccagata atgttgcgat gacagtgtca aactggttat tccttcaagg ggtgagttgt
    15421 tcttaaggaa agcataaaaa aaacatgcat acaacaatca gaacggttct gtctgcttgc
    15481 ttttaatgcc ataccaaacg taccattgag acacttgttt gcacagagga tggcccatgt
    15541 tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat gtctgtcggg
    15601 ctagcttgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg atcgtttctg
    15661 ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat gtggtgatga
    15721 tgacgctgga gctggctgac gggcgcattc cggtgattgc cgggaccggt gctaacgcta
    15781 ctgcggaagc cattagcctg acgcagcgct tcaatgacag cggtatcgtc ggctgcctga
    15841 cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat ttcaaagcca
    15901 tcgctgaaca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt actggctgcg
    15961 atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc ggaatcaaag
    16021 aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca gatgattttg
    16081 ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattaggc ggtcatgggg
    16141 ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc aaactggcag
    16201 cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca ttacacaaca
    16261 aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa ctgggtcttg
    16321 tggcgaccga tacgctgcgc ctgccaatga cacctatcac cgacagtggt cgtgagacgg
    16381 tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt gatggcttac
    16441 tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt attactcgct
    16501 gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc ctacctggaa
    16561 gcggcaccgc ttgcggagct tcatgccccg gctggaatga tcttgccggt gacctccggt
    16621 gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct ggacattcgt
    16681 ccaccagccc agccgctggc actggtttct ggcgcgcgta cccagttcac gggcgatacc
    16741 gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggctgcaggt ggttagcgtg
    16801 ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac actgaccacc
    16861 gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg ttatcaaatc
    16921 tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa cctggaacag
    16981 gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga gatgatgaac
    17041 gttatttccg ccggtctgga taaatctgcc actgacgccg cgaacgctgc gcaaaatcgt
    17101 gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc aatgctggtc
    17161 gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag ctgcgctaga aaaagtgggc
    17221 atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta taagccgctg
    17281 tctgacagcg aatggcacga actgggcgcg agcgatccag gtctggcatc cggtgactat
    17341 aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga tccgaaaggt
    17401 catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc tgcgtttagc
    17461 aagtaaaaat acagggctgg agtcatccgg cccttttttc tgatatgata cgcaaacgtg
    17521 tgcgtcgaca gaaaaacgcg attttagcgg taattcgcac gaaatttgtt tgtcggacgt
    17581 aggtcggata aggcgtttac gccgcatccg acaaaacatc cggcacacca gacagcaaaa
    17641 gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa gctgagttgt
    17701 atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg gtgctcgaat
    17761 tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat cgcaaaggta
    17821 tggtgaacaa caagttcaac tacttcatta tgagcaaact ggctgaagcg ggtatcccga
    17881 ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg gatatggtgc
    17941 cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct ggtgaaacgt cttggaatcg
    18001 aagaaggtat tgagctgaac ccgccactgt tcgatctgtt cctgaaaaac gacgccatgc
    18061 acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtgagc aaagagaacc
    18121 tggcgcgtat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa ctgttcgatg
    18181 atgctggtct cattctggtc gacttcaagc tggagtttgg cctgtacaaa ggtgaagtgg
    18241 tactgggcga tgagttctcc ccggacggca gccgcctgtg ggacaaagaa acgctggaga
    18301 aaatggataa agaccgtttc cgccagagcc tcggtggcct gatcgaagcc tatgaagccg
    18361 ttgcccgccg cctgggtgta cagctggact gatttttctg ttcatcatct tgccgtgctg
    18421 ctggcacggc aagacaaccg ctctcataag atgtgcattg aacgctcatt cctccgccat
    18481 ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt tctataatga
    18541 atataatgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca atgttgaaga
    18601 caggcgcaat agctccggcg gtccgtcaat gggcggtccc ggttttcgcc tgccaagcgg
    18661 taaaggcggg ctgattttac tgatagtcgt actggttgca ggctactatg gcgttgattt
    18721 aaccgggttg atgaccggac agccggtttc ccaacagcaa tcaacgcggt caattagccc
    18781 taatgaagac gaagccgcaa aattcacctc ggtgattctg gcaaccacgg aagacacctg
    18841 gggacagcaa ttcgagaaga tgggtaaaac ctatcagcaa ccgaagctgg tcatgtaccg
    18901 tggaatgacg cgtaccggct gcggggcggg ccagtccata atggggccat tctattgccc
    18961 ggcggatggc acagtttata tcgatctctc cttctatgat gacatgaaag acaaacttgg
    19021 cgcggatggc gattttgccc aggggtacgt tatcgctcat gaagtcggtc atcatgtgca
    19081 gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga
    19141 agtaaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg gtgtctgggg
    19201 gcatagtatg cagcagcaag gtgttctgga aaccggcgat ctggaagagg cgctgaacgc
    19261 ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag tagtaccaga
    19321 cagtttcact catggcactt ctcagcaacg ctacagctgg tttaaacgtg gtttcgacag
    19381 cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg ggcagggatg
    19441 gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg gatccgccgc
    19501 ttgctggtgt tgagcgggga agagcgttgg tgttttgatc atgcgcttaa gttgcgtgat
    19561 gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa ccactgttct
    19621 ccctcggcac tacaaacttt acttgggcgc gagttccggc atgcggtatt cgacgcccgc
    19681 caaggctttg atgccgctgc ctttgcggca cttagcggaa cgttgaaagc gggaagctgg
    19741 ctggtgttgt tactccctgt atgggatgag tgggaaaacc aacctgatgc cgactcgctg
    19801 cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca tttcaaacgc
    19861 gtacttacgg cgaataacga cgctatcctc tggcggcaaa accagccgtt ctcgttggcg
    19921 cattttactc cccgtactga ctggcacccc gctaccggcg caccacaacc agaacaacag
    19981 caactcttac agcagctact gaccatgccg ccgggcgtgg cagcggtaac tgctgcgcgt
    20041 gggcgcggta agtcggcctt ggcagggcaa ctcatttctc gtattgcggg cagtgcgatt
    20101 gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg cgagaagttt
    20161 cgctttattg caccggatgc cttgttagcc agcgatgagc aagccgattg gctggtggtc
    20221 gatgaagccg cagctatacc tgcgccgttg ttgcatcaac tggtatcgcg ttttcctcga
    20281 acgttgttaa ccactacggt gcagggctac gaaggcaccg gacgtggttt tttgctgaaa
    20341 ttttgcgctc gctttccgca tttacaccgt tttgaactac aacagccgat ccgttgggcg
    20401 cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttgacga tgaaaacttc
    20461 acccatacac cacaaggcaa tatcgttatt tccgcatttg aacagacgtt atggcgaagc
    20521 gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta ccggacttcg
    20581 ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca ggcggctggc
    20641 gaaaacgaga ttgccggggc gctgtggctg gtggatgagg gcggattatc tcaagaactc
    20701 agtcaggcgg tatgggcagg tcttcgtcgc ccgcggggta atctggtggc ccagtcgctg
    20761 gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gaaggcgggt cagccgaata
    20821 gcagttcatc cggctcgtca gcgggaaggc acaggtcagc aactgattgc tggtgctttg
    20881 caatatacgc aggacctcga ctatctttcg gtgagttttg gttacaccgg ggaattatgg
    20941 cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg gtaatcatcg tgaagccagc
    21001 agcggttgct atacggcgat ggcactgtta ccgatgagtg atgcgggtaa acagctggct
    21061 gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg gaatggcgaa
    21121 atgcttcctg ttgatccact aaacgatgcc gtcctttctg acgacgactg gcttgaactg
    21181 gccggttttg ctttcgctca tcgtccgcta ttaacgtcgt taggttgctt aatgcgtctg
    21241 ttacaaacca gcgaactggc attaccggcg ctgcgtgggc gtttacagaa aaacgccagt
    21301 gacgcgcagt tatgtaccac acttaaactt tcaggccgta agctgttact ggttcgccag
    21361 cgggaagagg ccgcgcaggc gctattcgca cttgatgatg ttcgcactga gcgtctgcgc
    21421 gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc catggtcatt
    21481 gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga aacatgacca
    21541 ttttgttgtt caaagcccgg ataaaccagc acaacagttg ttactgcttt tccacggtgt
    21601 cggggataac ccggtggcaa tgggggaaat aggcagctgg tttgcgccgc tgtttcctga
    21661 tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt aatccggcgg ggcgtcagtg
    21721 gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg ccatcatgcc
    21781 gacgtttatt gagacggtgc gctactggca gaaacaaagc ggagtagggg ctaatgccac
    21841 ggcgctaatc ggtttctcac aaggcgcgat tatggcgctg gagagcatta aagccgaacc
    21901 gggccttgct tcgcgcgtca ttgccttcaa cggacgctac gcgagcctgc cggaaaccgc
    21961 gtctacagcc acaacaattc acttgattca tggcggtgaa gatccggtta tcgatctggc
    22021 gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt ggcgatgtga cgctggatat
    22081 cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caattagccc tggatcactt
    22141 gcgttatacc attccgaaac attatttcga cgaagcgcta agcggcggta agcctggcga
    22201 tgatgatgtg attgaaatga tgtaataata gccgggtagg tctgataaga ctgtatcagg
    22261 catcatggcc ggatgcggcg ttaacgcctt atccggccac ttagcttact tcttcggttg
    22321 atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg
    22381 aagctccggc ttgttggcga gaaatttctt atggtcgacg cgcttgagat ctttaatcac
    22441 gttcagcagg acgcctacca aaaagacaat caccagaatc caccaatatt tagccagcca
    22501 gtccatgctt atttcctctt gcagaaccac tcatcaggcg acgagctgtt ccatgatacg
    22561 ttgatacata cgggcaagta gctgcaggtc ggcagcgttc acacattcat taattttatg
    22621 aatcgtggca ttgaccggcc cgagttccac cacctgcgcc cccatgcggg caataaagcg
    22681 cccgtcggac gttccgcctg tggtcagtag ctgcggttta atttcattat agtgctcaac
    22741 cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag
    22801 ccaccaatcc accgtatagc gcagttgatg tttttcaagc agggcaagca cctgcgcttt
    22861 gatcatctca tccgtcagtt cggtgctaaa gcggaagtta aactgcacaa acagttcacc
    22921 tggaataacg ttgttactgc ccgttcccgc ctgaatattg gcaatctgca tactggtcgc
    22981 cgggaagaat tcattaccct gatcccactc aatagccact aattcattaa tgaaaggtgc
    23041 tgcgcgatgt accggattgt cagccagatg tgggtaggca acatgcccct gaacgccatg
    23101 aatggtaagg ttgcaggtta acgatccgcg acgaccattt ttcaccacat cacctaccac
    23161 ttcgatactc gacggttcgc caaccagaca gtaatcgaga cgctcattac gcgccattaa
    23221 cgcttcgacg acttttaccg taccattgtg ggcactagct tcttcatcag aggtgatcag
    23281 aaatgccagt cgccccgtat ggttgggatg ttgtgcgaca aagcgttctg ccgctactac
    23341 catcgccgcc agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca tgccgtcacg
    23401 aatggttggt tcaaacggcg gattgatcca acgagcggcg tcgccaggcg gcaccacgtc
    23461 ggtatgcccg gcaaaggcca atgtttcacc ttgcccacgc catgcccaaa aattctgcgt
    23521 atcggcaaag tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa
    23581 agcctggcat cctgcgtcat caggactcag ggaagggcgg cgaataagct gttgtgtcag
    23641 ctcaataacc gggcacgaca tagactacac ctcatggaaa aattgctgat aactggattc
    23701 actgaaaccc agcagcatag gcttaccagg cgcgcagagc aatggacgtt tgataattgc
    23761 aggcatttca gtcattaatg tcgctgcaga ggccgcatcg gtgattttat tgcgggtggt
    23821 ttcgtccagt ttacgccagg ttgtaccacg ggtgttgagt aacgcttccc agcctaattc
    23881 gttgataaaa ccgttcaata attcgctgtc caatccatcg acgcggtaat catgaaaacg
    23941 atagtcgatg ttattagctt ccagccaacg gcgagccttc ttaatggtgt cacaattttt
    24001 gatgccgtaa agtgtaacca taatgaatcc tttttcgatt tatgagaata aatactcatt
    24061 taaaggtgaa aacatttt
//
LOCUS       DAZFRG010000054        23976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFRG010000054 DAZFRG010000000
VERSION     DAZFRG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.054"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..683
                     /locus_tag="ACHKUC_001669"
     CDS             <1..683
                     /locus_tag="ACHKUC_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617938.1"
                     /translation="NLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSKI
                     EALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQYF
                     ILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINALA
                     KWQQLLFSTSMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMANIQFDRLQPAE
                     SPQRKNKK"
     gene            727..5322
                     /locus_tag="ACHKUC_001670"
     CDS             727..5322
                     /locus_tag="ACHKUC_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6617939.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVVGG
                     LLSSGVQASTALDGGGASESTPLTDTWIAIGQGAKASSVDTSPSGGPGTSSIAIGQEA
                     QSGIGSTSLGFKSQATGERSVALGQTADATGNRAIAIGSGAKSTADYSLTLGNGSKAF
                     AENATAIGKDARAEGKGSQAFGKVALATGENAIALGSNSNASGDNAVALGSTSQAYGD
                     SSLAIGELSETNAENAIALGKLSNASKINSIALGSNSTASGVGSVALGENSFAGGINS
                     LALGSQSNANGDNAVALGVGSVAEQDNTVSVGNSTTQRKITNMAAGLILPGSTEAING
                     SQLHALSASVAARLGAGAGVNGDGSINAPSYKLKSGTYNNVGEALLGIDNDTLHWDKT
                     EGAFSASYIVKNADGSPKSSIAQNKIINVAKGTISATSTDVVNGSQLYDLQQDALLWN
                     GTAFSAAHGTEATSKITNVEDGTISDTSKDAVNGSQLKETKDDVATNTANIATNTTDI
                     ATNTANIADNTANIATNTTDIATNTADIATNASSITTLNADVDTLEKDALLWNGTAFS
                     AKHGTEATSKITNVTAGDLTAGSTDAVNGSQLKTTNDNVSTNTTNITNLTDSVGDLKD
                     DSLLWNKTAGAFSAAHGTEATSKITNLLAGKVSSDSTDAINGSQLYGVADSFTSYLGG
                     GADISDAGVLTGPTYTIGGTDYTNVGDALAAINTSFSSSLGDALLWDATAGKFSAKHG
                     INNDPSVITDVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNADGSITTPTYA
                     IAGGSYDNVGDALEAIDTTLDDALLWDETANGGNGAFSAAHGKDKTASVITNVANGAV
                     SATSSDAINGSQLYSTNKYIADALGGDAEVNADGTITAPTYTIANTDYNNVGEALDAL
                     DNNALLWDATANNGAGAYSASHDGKASIITNVADGNIGEDSTDAINGSQLNATNMMIQ
                     QNSEIINQLAGDTSKNYIGDNGAGINYVRTNDSGLAFNDASAAGIGSTAVGYNTVAKG
                     DSSVAIGHGSNSIVETSIALGSESVSSRLIVKGSRNTRVTENGVEIGYDTTDGELLGA
                     LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVG
                     EDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTTR
                     GAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVS
                     QNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSVAI
                     GSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSS
                     EAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETK
                     QYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAV
                     ALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5734..7389
                     /gene="lldP"
                     /locus_tag="ACHKUC_001671"
     CDS             5734..7389
                     /gene="lldP"
                     /locus_tag="ACHKUC_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6617940.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7389..8165
                     /gene="lldR"
                     /locus_tag="ACHKUC_001672"
     CDS             7389..8165
                     /gene="lldR"
                     /locus_tag="ACHKUC_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO6617941.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            8162..9352
                     /gene="lldD"
                     /locus_tag="ACHKUC_001673"
     CDS             8162..9352
                     /gene="lldD"
                     /locus_tag="ACHKUC_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO6617942.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9400..9873
                     /gene="trmL"
                     /locus_tag="ACHKUC_001674"
     CDS             9400..9873
                     /gene="trmL"
                     /locus_tag="ACHKUC_001674"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO6617943.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(9928..10749)
                     /gene="cysE"
                     /locus_tag="ACHKUC_001675"
     CDS             complement(9928..10749)
                     /gene="cysE"
                     /locus_tag="ACHKUC_001675"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO6617944.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRAQAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10829..11848)
                     /gene="gpsA"
                     /locus_tag="ACHKUC_001676"
     CDS             complement(10829..11848)
                     /gene="gpsA"
                     /locus_tag="ACHKUC_001676"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO6617945.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11848..12315)
                     /gene="secB"
                     /locus_tag="ACHKUC_001677"
     CDS             complement(11848..12315)
                     /gene="secB"
                     /locus_tag="ACHKUC_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO6617946.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12378..12629)
                     /gene="grxC"
                     /locus_tag="ACHKUC_001678"
     CDS             complement(12378..12629)
                     /gene="grxC"
                     /locus_tag="ACHKUC_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO6617947.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12770..13201)
                     /locus_tag="ACHKUC_001679"
     CDS             complement(12770..13201)
                     /locus_tag="ACHKUC_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6617948.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13446..14990
                     /gene="gpmM"
                     /locus_tag="ACHKUC_001680"
                     /gene_synonym="pgmI"
     CDS             13446..14990
                     /gene="gpmM"
                     /locus_tag="ACHKUC_001680"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO6617949.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15024..16283
                     /gene="envC"
                     /locus_tag="ACHKUC_001681"
     CDS             15024..16283
                     /gene="envC"
                     /locus_tag="ACHKUC_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO6617950.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLSERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16287..17246
                     /locus_tag="ACHKUC_001682"
     CDS             16287..17246
                     /locus_tag="ACHKUC_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO6617951.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(17233..18267)
                     /gene="waaH"
                     /locus_tag="ACHKUC_001683"
     CDS             complement(17233..18267)
                     /gene="waaH"
                     /locus_tag="ACHKUC_001683"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO6617952.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTGQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18506..19531)
                     /gene="tdh"
                     /locus_tag="ACHKUC_001684"
     CDS             complement(18506..19531)
                     /gene="tdh"
                     /locus_tag="ACHKUC_001684"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO6617953.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19541..20737)
                     /gene="kbl"
                     /locus_tag="ACHKUC_001685"
     CDS             complement(19541..20737)
                     /gene="kbl"
                     /locus_tag="ACHKUC_001685"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO6617954.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            20951..21883
                     /gene="rfaD"
                     /locus_tag="ACHKUC_001686"
     CDS             20951..21883
                     /gene="rfaD"
                     /locus_tag="ACHKUC_001686"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO6617955.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            21893..22939
                     /gene="rfaF"
                     /locus_tag="ACHKUC_001687"
     CDS             21893..22939
                     /gene="rfaF"
                     /locus_tag="ACHKUC_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO6617956.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHILREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHAAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            22943..23923
                     /gene="rfaC"
                     /locus_tag="ACHKUC_001688"
     CDS             22943..23923
                     /gene="rfaC"
                     /locus_tag="ACHKUC_001688"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO6617957.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
BASE COUNT         6025 a         6241 c         6228 g         5482 t
ORIGIN      
        1 tgaacctaaa gaaaacactg ttaagcgtgt taatgatatt gcaactttgc ttattggtag
       61 ggtgtgacta tattgaaaaa gcgagtaagg tcgacgatct cgttacacag caagagttac
      121 aaaaaagcaa aattgaggcg cttgaaaaac aacaagaact cgacaagcgc aagatagaac
      181 actttgaaaa acaacaaact accatcataa acagtaccaa aacgctcgct ggcgtggtga
      241 aggcagttaa agacaaacag gacgaatttg tctttacaga atttaacccg gcacaaaccc
      301 aatacttcat tttaaataac ggttctgttg gtttggcagg gaaaatactg tctattgacg
      361 cagtagaaaa cggcagcgtt attcgtattt cactggttaa cttattaagt gttcctgtat
      421 caaatatggg tttttacgcg acatgggggg gagaaaaacc caccgacatc aacgcattag
      481 caaaatggca gcaattgcta tttagtacct caatgaactc ctccctgaaa ttattaccag
      541 gtcaatggca agacattaat ttgacgctaa aaggtgtctc gcccaacaac ctcaaatatc
      601 tgaaattagc catcaacatg gcaaatattc agttcgaccg tcttcaacct gctgaatctc
      661 cacagcggaa aaacaaaaaa taaatgcagc ttctgcgctt atatcaagga atagagagca
      721 tcaataatga acaaaatatt taaagttatc tggaatccgg caacaggtag ttacaccgtt
      781 gccagtgaaa cagcaaaaag tcgtggtaaa aaaagcgggc gcagtaagct gttaatttct
      841 gcgttggttg tgggtgggtt gttgtcgtct ggagtacaag catcgaccgc gcttgatggt
      901 ggtggggcta gcgaaagcac tccattgacc gatacctgga ttgccattgg tcagggtgct
      961 aaagcatcat cagtagacac ttcaccatca ggaggaccag gaacatcaag tattgcaata
     1021 gggcaagaag cccaatctgg gataggtagt acctcattag gtttcaagag ccaggctaca
     1081 ggagaaagat ctgttgcttt aggacaaaca gctgacgcaa ccggtaatag ggccatagca
     1141 atcggtagtg gagctaaatc gactgccgat tattccctta cgctggggaa tggatctaag
     1201 gctttcgctg aaaacgccac ggcgataggc aaggacgcaa gagcggaggg caaaggtagt
     1261 caggccttcg gaaaagtggc cttggccact ggggagaatg ccattgcctt gggtagtaac
     1321 agcaatgcat ctggtgataa cgccgttgcg ctgggtagta caagtcaggc atatggcgac
     1381 tccagccttg caataggtga gttaagtgag accaatgctg aaaatgctat tgcactggga
     1441 aaactcagta acgcaagtaa aatcaattca atcgccctgg gtagtaacag cactgcatct
     1501 ggtgtgggct ccgttgcgct gggagagaat agttttgctg gaggcataaa cagtcttgct
     1561 ttgggtagtc aatccaatgc aaacggtgat aacgcagttg ccctcggtgt aggatctgtc
     1621 gcagagcaag acaataccgt ttcggtaggg aacagcacaa cacaacgcaa aattaccaat
     1681 atggctgccg gtcttatact accaggcagt actgaggcca ttaacggttc acaacttcat
     1741 gcattaagtg cctcagtcgc agcccgactc ggggctggtg ctggcgtaaa tggagatgga
     1801 agtataaatg cgccaagcta caagctgaaa agcggcacct acaataatgt gggagaggcg
     1861 cttttgggaa tcgacaatga cactctgcac tgggacaaaa ccgagggagc atttagtgcc
     1921 agctatatcg tcaaaaacgc tgatggcagc ccaaagagca gcatagccca aaacaaaatc
     1981 attaacgttg caaagggtac tatttccgcc accagcaccg atgtagtaaa cggctctcaa
     2041 ttgtacgacc tgcagcagga tgcgctgttg tggaacggca cagcattcag tgccgcacac
     2101 ggcaccgaag ccaccagcaa aatcactaat gttgaagatg gtactatttc agacaccagt
     2161 aaagatgcgg ttaacggttc ccagttgaaa gaaaccaaag acgatgtggc caccaacaca
     2221 gccaatatcg ccactaacac cacggatatt gcaaccaaca ctgccaatat cgccgataac
     2281 actgccaata tcgccactaa cactacggat attgcgacta ataccgccga tatagctaca
     2341 aacgctagca gtatcactac tctgaacgcg gacgttgata ccctggaaaa agatgccctg
     2401 ctctggaatg gcacagcatt cagcgccaaa cacggcaccg aagctaccag caagatcacc
     2461 aatgttaccg ctggcgacct gactgctggc agcactgacg ccgttaatgg ctcccagctc
     2521 aaaaccacca acgataacgt gtcgaccaac accaccaaca tcaccaacct gacggattcc
     2581 gttggcgacc ttaaggacga ttccctgctg tggaacaaaa cggctggcgc attcagcgcc
     2641 gcgcacggca ccgaagccac cagcaagatc accaacttac tggctggcaa ggtatcttct
     2701 gacagcaccg atgccattaa cggatcacaa ctttatggcg tagcggattc atttacgtca
     2761 tatcttggtg ggggtgctga tatcagcgat gcaggcgtat taaccgggcc aacctacact
     2821 attggtggta ctgactacac taacgtcggt gatgctctgg cagctattaa cacatcattt
     2881 agctcatcac tcggcgacgc cctactctgg gatgcaaccg caggtaaatt cagcgccaaa
     2941 cacggcatta ataatgatcc cagtgtaatc actgatgttg caaacggtgc agtctcgtcc
     3001 accagtagcg acgccattaa cggttcgcaa ctttatggcg ttagtgacta cattgccgat
     3061 gctttgggcg ggaacgctgt ggtgaatgca gacggcagta tcactacacc gacttatgcc
     3121 atagcgggcg gcagttacga caatgtcggt gacgcgctgg aagcgatcga taccacgctg
     3181 gatgacgccc tgctgtggga tgagacagcc aatggcggta acggtgcatt tagcgccgct
     3241 cacgggaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc agtctctgcc
     3301 accagtagcg atgccattaa tggctcacag ctctatagca ccaataagta catcgctgat
     3361 gcgctgggtg gtgatgcaga agtcaacgct gacggaacta tcactgcacc gacttacacc
     3421 attgcaaata ccgattacaa caacgtcggt gaagccctgg atgcgcttga taataacgct
     3481 ctgctgtggg atgcaacagc caataacggc gcaggtgctt acagcgccag tcatgacggc
     3541 aaggccagca ttatcacaaa cgttgctgat ggtaacattg gtgaagatag caccgacgct
     3601 atcaacggtt ctcagttgaa tgcaaccaac atgatgatcc agcagaacag cgaaatcatc
     3661 aatcagcttg ccggtgacac cagtaaaaac tacatcggag ataacggtgc aggtattaac
     3721 tatgtacgta ccaacgacag cggcttagcg ttcaacgatg ccagcgcagc aggtattggc
     3781 tctaccgctg tggggtataa cactgttgcc aaaggcgata gcagcgtggc aatcggtcat
     3841 ggcagcaaca gcatcgttga gacgagtatt gcgctgggta gcgaatccgt ctccagccgt
     3901 ttgattgtga aaggctcccg taatacccgc gttacggaaa atggggtcga gatcggttat
     3961 gacaccacgg atggcgaact gcttggcgcg ttgtcgatcg gtgatgatgg taaatatcgt
     4021 caaatcatca acgtcgcgga tggttctgaa gcccatgacg ctgttactgt tcgccagttg
     4081 caaaacgcca ttggtgcagt agcaaccaca ccaaccaaat actatcacgc caactcaacg
     4141 gctgaagact cactggcagt cggtgaagac tcgctggcaa tgggcgcgaa aaccatcgtt
     4201 aatggtaatg cgggtattgg tatcggcctg aacactttag ttctggctga tgcgatcaac
     4261 ggtattgcta tcggttctaa cgcacgcgca aatcatgccg acagcattgc aatgggtaat
     4321 ggttctcaga ctacccgtgg tgcgcagacc aactacactg cctacaacat ggatgcaccg
     4381 cagaactctg tgggtgagtt ctctgtcggc agtgaagacg gtcaacgtca gatcaccaac
     4441 gtcgcagcag gttcggcgga taccgatgcg gttaacgtgg gtcagttgaa agtaacggac
     4501 gcgcaggttt cccagaatac ccagagcatt actaacctga acactcaggt cactaatctg
     4561 gatactcgcg tgaccaatat cgaaaacggc attggcgata tcgtaaccac cggtagcact
     4621 aagtacttca agaccaacac cgatggcgca gacgccaacg cgcagggtaa agacagtgtt
     4681 gcgattggtt ctggttccat tgctgccgct gacaacagcg tcgcactggg cacgggttcc
     4741 gtagcagacg aagaaaatac gatctctgtg ggttcctcta ccaaccagcg ccgtatcacc
     4801 aacgttgctg ccggtgttaa tgccaccgat gcggttaacg tttcgcaact gaagtcttct
     4861 gaagcaggcg gcgttcgcta cgacaccaaa gctgatggct ctatcgacta cagcaacatc
     4921 actctcggtg gtggtaacgg cggtacgact cgcatcagca acgtttctgc tggcgtgaat
     4981 aacaacgacg cggtgaacta tgcgcagttg aagcaaagtg tgcaggaaac gaagcaatac
     5041 accgatcagc gcatggttga gatggataac aaactgtcca aaaccgaaag caagctgagt
     5101 ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttacac gccgggtgcc
     5161 agcatggcgt ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgtg
     5221 tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag
     5281 ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat taacgaatgg
     5341 gtaaacgcca cattgcctga tgcgctacgc ttatcaggcc tacggggtga ttcagcacat
     5401 tcctgtaggc cggataaggc gttcacgccg catccggcaa gataaggcgc accagatcaa
     5461 caatctgagg gcaattctct gatgggaatt gcccttttct ttattacctc tctcccagaa
     5521 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
     5581 acatcaatgt tgccaacaca taacatttag ttaaccattc attgtcatta cccctacaca
     5641 acacaattgg cagtaccact tttacacaac gtgtgacaag gagatgggca acagactcat
     5701 tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca aaactacgat
     5761 cccgccggga atatctggct ttccagcctg atagcatcgc ttcccatcct gtttttcttc
     5821 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtggcaatc
     5881 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
     5941 gtttatggct tcttctacgg gttgtggccc atcgcgtgga tcattattgc agcggtgttc
     6001 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
     6061 ataacccctg accagcgcct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt
     6121 gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggtctg
     6181 ggctttaaac cactgtacgc cgccgggctg tgcctgattg ttaacaccgc gccagtggca
     6241 tttggtgcga tgggtattcc gattctggtc gccgggcagg taacaggcat cgacagcttt
     6301 gagattggtc agatggtggg gcgtcagctg ccgtttatga ccattatcgt gctgttctgg
     6361 atcatggcga ttatggacgg ctggcgcggt atcaaagaaa cctggcctgc ggtcgtggtt
     6421 gcgggcggct catttgccat cgctcaatac ctcagctcta actttattgg gccggaactg
     6481 ccagacatta tctcttcgct ggtatcgctg ctctgtctga cactgttcct caaacgctgg
     6541 caaccagttc gcgtattccg cttcggtgac ttaggggcgt cacaggttga tatgacgctg
     6601 gctcacaccg gttacacggc aggtcaggtg ctgcgcgcct ggacaccgtt cctgttcctg
     6661 accgccaccg taacgctgtg gagtatcccg ccgtttaaag ccctgttcgc ttcaggcggt
     6721 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact ggttgcccgt
     6781 atgccgccag tggtcagcga agcgacagcg tatgccgccg tgtttaagtt tgactggttc
     6841 tctgccactg gcaccgccat tctgtttgct gccctgctct cgattgtctg gctgaagatg
     6901 aaaccatctg acgctatcag caccttcggc agcacgctga aagaactggc tctgcccatc
     6961 tactccatcg gtatggtgct ggcattcgcc tttatctcga actattcagg actgtcatca
     7021 acactggcgc tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
     7081 ggctggcttg gggtattcct gaccgggtcg gatacgtcat ctaatgcttt gtttgccgct
     7141 ttgcaggcta ccgccgcaca acaaattggc gtttctgact tgttgctggt tgctgccaac
     7201 accaccggtg gtgtcaccgg caaaatgatc tctccgcaat ctatcgctat cgcctgtgcg
     7261 gcggtaggtc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
     7321 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cctatgtctt aacgtggatg
     7381 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
     7441 cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgaa cgtcaactgg
     7501 cgatgcaact cggcgtgtcg agaaattcac tgcgcgaggc gctggcgaaa ctggtaagcg
     7561 aaggtgtgct gctcagtcga cgtggcggcg ggacgtttat tcgctggcgt catgacacat
     7621 ggtcggagca aaacatcgtc cagccgctga aaacgctgat ggccgatgat ccggattaca
     7681 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcagcaa
     7741 tgcgtgccac gcctggcgaa aaagaaaaga ttcagctttg ttttgaagca acgctcagcg
     7801 aagacccgga tctcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
     7861 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctctg
     7921 tgaagcatag ccgccagcgg atgtacctgg tacccccagt tttttcacag ctgaccgaac
     7981 aacatcaggc ggtcattgac gccatttttg ccggtgatgc tgacggggcg cgtaaagcaa
     8041 tgatggcgca ccttagcttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
     8101 gccatgcacg cattacccgc ctgcctggtg accataatga gcattcgagg gagaaaaacg
     8161 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
     8221 cctgttccac tatatggatg ggggggcata ttctgaatac acgctgcgcc gcaacgtgga
     8281 agatttgtca gaagtggcgc tgcgccagcg tattctgaaa aacatgtctg acttaagcct
     8341 ggaaacgacg ctgtttaatg agaaactgtc gatgccggtg gcgctaggtc cggtaggttt
     8401 gtgtggcatg tatgcgcgac gcggcgaagt tcaggctgcc aaagccgcgg atgcgcatgg
     8461 cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtgg ctccggctat
     8521 caaacgtccg atgtggttcc agctttatgt gctgcgcgat cgcggcttta tgcgtaacgc
     8581 actggagcga gcaaaagcag cgggttgttc gacgctggta ttcaccgtgg atatgccaac
     8641 accgggagcg cgttatcgtg atgcgcattc tgggatgagc ggcccgaacg cggcaatgcg
     8701 ccgctatttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg
     8761 tccgcatgat ttaggtaata tctcggctta cctcggcaaa ccgaccggac tggaagatta
     8821 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat
     8881 ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg
     8941 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
     9001 ggacggtgtg ctgtcttctg cccgtgcgtt gcctgctatt gcagatgcgg tgaaaggtga
     9061 tatcgccatt ctggcggata gcggaatacg taacgggctt gatgtcgtgc gtatgatcgc
     9121 gctcggtgcc gacaccatac tgctgggtcg tgctttcctg tatgcactgg caacagcggg
     9181 ccaggcgggt gtagctaatc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
     9241 gctgactggc gcgaaatcga ttagcgaaat tacgcaagat tcgctggtgc aggggctggg
     9301 taaagagttg cctgcggcac tggctccgat ggcgaaaggg aatgcagctt aagggttaga
     9361 cgaatatctg ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac
     9421 gaaccagaaa ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt
     9481 cgtctgcata tcatcgaacc gatgggattt gcctgggacg ataagcgcct gcgccgcgcg
     9541 gggctggact atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgtttctc
     9601 gaagcagaaa atccccagcg tctgtttgcc ctcaccacga aaggtacgcc tgctcacagc
     9661 gccgtaagct atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca
     9721 gcgagcattc ttgatgccct gcccgctgaa caaaaaattc gtattccaat ggtgccggac
     9781 agccgcagca tgaacctgtc caatgcggtg tcggtggtgg tgtatgaagc ctggcgccag
     9841 ctggggtatc cgggagcggt gttgagagat taagatgcgg taggccggat aaggcgttca
     9901 cgccgcatcc ggcacgatca cagaatgtca gatcccatca ccatactcaa acgtatggtt
     9961 aatgccgttg aaatgctgat ccatatccat tgacggttta tcgctatccg gcttgccgac
    10021 aatgcgtgcc ggaacgccag cggcagtggt gtgcggcggc accggttgca gcaccacgga
    10081 acctgcgcca atcttcgcgc cgcgcccaac ttcaatattg ccgagaattt tcgcgcccgc
    10141 gccaatcatc acgccttcac gaattttcgg gtgacggtca ccaccagatt tacccgtacc
    10201 gccaagcgtc acagattgca gaatcgatac gtcgttttca atcaccgccg tttcaccaac
    10261 gacgatgcct gtcgcgtggt cgagcatgat accgcgacca atttttgctg ccgggtgtat
    10321 atcaacctgg aacgttacag agacctggtt ttgtagaaaa atagcctgcg cgcgacggcc
    10381 ctgattccac aaccagtgac cgatgcgata cgcctgcaaa gcatgaaaac ccttcagata
    10441 tagcaacggg gttgagtatt tatccaccgc cgggtcgcgg gtacgcactg cctgaatatc
    10501 acaggccgca gaagcgatca tttccgggtc ggcggcgtag gcctcttcca ccacttcgcg
    10561 gatagcaatg gcaggcataa ttggcgatga cagcttgttc gccagcatgt agctcagtgc
    10621 actgcccagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca gcattggctc
    10681 acaatccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt ccagttcttc
    10741 acacgacatt gcttactcca cacgataaga taatgaccga cccgttctgc gcgggccggg
    10801 tcatagtggt aacaaaggtt ccctgggttt agtggctgct gcgctcgtcc ttgcgtgcac
    10861 gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc gcaatataat acttgataaa
    10921 tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg acttctttcg
    10981 tattgcggta gccttccacc acttgaccaa ttttctcctg cgcgctttgt acatccatgc
    11041 cctgaccgag catcatgcca aaacggcggt tacgcgactg gttgtcggta caggtaagca
    11101 ccagatcgcc cagccccgcc attcccataa aggtggcagg atcggcaccc agcgccgcgc
    11161 caagacgcga catttcagcc agtccacggg tgatcagcgc cgtacgcgca ttcgcgccaa
    11221 aaccgatacc gtcggacatc cccgcgccaa tggcaatgac gtttttcacc gcaccgccaa
    11281 gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa acttttgcca cagtgcagca
    11341 attgctggag gtcatcggca aaggtctggt ccgtcgaggc cagcgaaatg gccgtcggta
    11401 aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc ggaatttgat
    11461 cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttctgct tccagccctt
    11521 tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg cgcagcactt
    11581 caccaaagac atggctgggt acgacgacga gaatattacg gctggctgcc agcgcagtgg
    11641 cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga aacgcggcgt
    11701 tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag aggacaacct
    11761 cgtggccatt tcttgccagg gtgatggcaa gagcggtgcc gtacgagccg gcaccgatca
    11821 cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag taccttcgcc
    11881 agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg caaggttcag
    11941 ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat acgggaacag
    12001 aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga tacccgcgat
    12061 ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc ccagggaggc
    12121 cgttacggta acacgcagta ccacttcgta aacgtcatct gccagttgag tagatgccgt
    12181 atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg gcgcgttcgg
    12241 cgcttcgaaa gaaatatcct tggtataaat acgttggatc tggaaagtca tttcggtgtt
    12301 gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact gcgcaatgta
    12361 atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc atccaatgca
    12421 tacaagtcat cacagccacc aatgtgctgt gcgtcaataa aaatctgtgg aaccgtggtg
    12481 cgaccactgc gtttgatcat ctcttcacgc ttagcggcat tgccatcgat cggcaactcc
    12541 tgaaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata cgggcaggtt
    12601 tctttggtat agatttcaac attggccata aattagctcc tgttttttac cctgaaattt
    12661 catgttgcag ggcggcagca aaatggagtg tccccagaag cttatatcaa taagtcgctg
    12721 ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc
    12781 gcaccaaagg caagttttcg ccagcccagc cagcgacgcc ttctttcagt acgaatactt
    12841 gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccat
    12901 ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg ttggctttga
    12961 tttcgctcgg caacaggttg atagaacctg cgatatggcc tttacggaag tcatcacgct
    13021 gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag
    13081 taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta
    13141 acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca
    13201 tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc
    13261 ggtgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc gcgacgaaaa
    13321 aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc
    13381 tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg
    13441 tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc
    13501 gtgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt
    13561 gggccaatcg tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc
    13621 gtcagatggg taactccgaa gtgggccacg ttaacctggg tgccggccgc atcgtgtatc
    13681 aggacctgac tcgtctggac gttgaaatca aagatcgcgc tttcttcgct aacccggtac
    13741 tgactggcgc ggtagataaa gcgaaaaacg caggcaaagc agttcacatt atgggtctgc
    13801 tctccgcagg cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggccg
    13861 ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacactccgc
    13921 cgcgcagtgc tgaatcctcg ctgaaaaaat tcgaagagaa atttgccgcg ctgggtaaag
    13981 gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc
    14041 gcgttgaaaa agcttatgac ctgctgactc tggcacaagg tgagttccag gccgataccg
    14101 ccgttgccgg tttgcaggct gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga
    14161 ccgttattcg tgcagaaggt cagccagatg cagcaatgga agacggcgat gcgctgattt
    14221 tcatgaactt ccgtgctgac cgcgcgcgtg aaatcactcg tgctttcgtg aacgctgatt
    14281 tcgatggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg
    14341 ctgccgacat caaaaccgca gtggcttacc cacccgcttc cctggttaac accttcggcg
    14401 aatggatggc gaaaaacgac aaaactcagc tgcgtatttc cgaaaccgaa aaatatgccc
    14461 acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc
    14521 tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc
    14581 tgaccgaaaa actggttgcg gcaatcaaga gcggcaaata cgacaccatt atctgtaact
    14641 atccgaacgg cgacatggta ggtcacaccg gagtgatgga agcggcggtt aaagcggttg
    14701 aagcgctgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac
    14761 tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg
    14821 cacacacagc acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga
    14881 aagcggttga aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta
    14941 tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca
    15001 tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca
    15061 ggcccatcat ctacgccagc gttctgagcg ctggcgtatt gttgtgcgcc ttttccgccc
    15121 acgcggatga gcgtgaccaa ctcaaatcca ttcaggctga catcgccgcg aaagagcgcg
    15181 cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag
    15241 aagcgatctc tgaagccacc cgtaaactgc gcgaaacgca aaacacgctt aatcaattga
    15301 ataaacagat tgatgagatg aacgcgtcga ttgccaaact agagcagcaa aaagccgccc
    15361 aggagcgcag cctcgccgcg caactggatg ccgcgtttcg tcagggtgaa cataccggta
    15421 ttcagctgat tctcagcggt gaagaaagcc agcgtgggca gcggttacag gcttatttcg
    15481 gctatctcaa ccaggcgcga caagaaacca ttgctcagtt gaaacaaacg cgtgaagaag
    15541 tcgctatgca gcgtgccgaa ctggaagaga aacagagcga gcaacaaacg cttttatatg
    15601 agcagcgcgc ccaacaggcg aagctgaccc aggtgttgag cgagcgtaag aaaacactgg
    15661 cagggctgga gtcttccatc cagcaaggtc agcaacagtt gagcgagctg cgcgccaacg
    15721 aatcccgtct gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac
    15781 gtgaagcgcg cgaagcccag gcggttcgcg accgccagaa agaagcgacg cgcaaaggca
    15841 ccacctacaa gccgaccgaa agcgaaaaat cgctgatgtc ccgtaccggt ggtctgggtg
    15901 cgccgcgtgg tcaggcattc tggccagttc gcgggccgac gctgcatcgc tatggcgagc
    15961 agctacaggg tgaattacgc tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag
    16021 ttaaagcaat tgccgacggt cgggtgattc tggctgactg gctgcaaggc tacggtctgg
    16081 tggtggtggt tgagcatggt aaaggcgaca tgagtcttta cggctataat cagagcgcac
    16141 tggtgagcgt tggttcgcag gttcgcgcgg gccagccgat tgcactggtg ggcagcagtg
    16201 gcggtcaggg cagaccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc
    16261 cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg
    16321 ccgccctgtt ggcgctctcc tcccccgtaa ttgctggcaa acttgccatc gtcattgatg
    16381 attttgggta tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg
    16441 tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagca cataacagcg
    16501 gtcacgaagt gttgattcat ctaccgatgg caccactgag taaacagccg ctggagaaaa
    16561 atacgctacg cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca
    16621 ataacgtgcc ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc
    16681 tgtttggtat gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca
    16741 gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca aggcactggc gtgaaggtga
    16801 tcaaacggaa ggtgttcctc gacgattcgc aaaatgaagc ggacatccgt gtgcaattca
    16861 atcgcgcaat tgacctggcg cgtcgcaacg gttcaaccat tgccattgga catcctcacc
    16921 cttcaacggt acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg
    16981 tgaaagccag cagcttgctg aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca
    17041 ctgtgcccga cacaccgcgt aatccattcc gtggcgtgaa gctgtgcaaa ccgaagaagc
    17101 cgctggaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga
    17161 gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg
    17221 caaaacttaa cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt
    17281 tttgtcgcgc cattgccata agcggaaaga ccacagtaac gcctggtaac ccaccttcac
    17341 gctgcgcaca ttggtaatca ggcgcttata cataccggaa gtaaatatct cagcaatcat
    17401 ccgttggcgg gtaataatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc
    17461 gtaggttatt tgctgatgaa attccggata aatcgtaatt ttgtcagcat aatttcgatt
    17521 taatttctcc agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt
    17581 cccttgtctg ttcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg
    17641 cccggtatat cgcgcacgca gcgcgttaaa cataaattct gttgtccaga caatatcctg
    17701 atgatgtaat ccggcaataa atttaatgtt atttttaaca ataacatcgc ggcgataaac
    17761 ccccatccag accacgtgtg tccaacggcg ggaagaaagt cccatccgca gccagtccgg
    17821 accagttaat acaccggttg agcgaaggcg atcggtaggg atggattgcc aggtttcacc
    17881 cgtttcacga aaacaccaat cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc
    17941 catggtcatc agcgtttcgt acatggtggg atacacctca tcgtcagcat cgacaaaagc
    18001 gacatatttt cccgttgcca cttcaatccc acgattacgc gccaccgatg cgccagcatt
    18061 cgcctgatgc aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac
    18121 agaattatcc gttgaaccat cgttaataat aatgatttcc agagcagtcc atgtttgcgt
    18181 aattaaagat tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac
    18241 actaagttta ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg
    18301 ttttacaggc tttgtattaa gaaactatta agcctgtgag atattatttt taccggtgag
    18361 agagtcacgt cagggtagag agataatgag agcagctata taagagatca gcgcaattac
    18421 gaactcttga ttacactgac aggattttac atatttaacg gattatcctc aggcaaaagg
    18481 gctggaatac cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc
    18541 ccgaacgcat ggcatcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga
    18601 tcggcgagag atcgaggcca gactgaatca gcgccgccat cttgtaccag gtttcaaaca
    18661 tctcacgacc gtaaatacct ttaatgaaca agcctttaaa gattactttg gtccagtcga
    18721 tagacatatc ggacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtgt
    18781 cgagcatggt acgaaacgct ggcggcgcac cggacatttc cagaccgaca tcaaagcctt
    18841 cggtcatgcc cagttctgcc atcacatcat taaggttttc tttagcgacg ttaaccgcac
    18901 gggtgatacc cattttgcgt gccagctcaa ggcggtattc gttaacatca gtgatcacca
    18961 cattgcgtgc accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac
    19021 cagagaccag cacatcctcg ccaaccagat cgaacgacag cgcggtatgc acggcgttac
    19081 cgaagggatc aaaaatggaa gccaggtcgt cggagatatt gtccgggatt ttgaaggcgt
    19141 tgaacgccgg aatcaccaga tactcggcga agcagcccgg acggttaaca ccgacgccga
    19201 tcgtgttgcg gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga
    19261 tatggccttc gccagaaacg cgatcgccaa tcttgaagcc tttcacttcc tgaccaatac
    19321 ctaccacttc accgacatat tcatggccca cgaccatcgg caccgggatg gttttttgcg
    19381 accactcatc ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt
    19441 taatcagcag atcgttatgc ccgagttccg gtacaggaac gtcggtcatc cagatgccct
    19501 cttccgcttt cagtttggat aacgctttca tctcacaccc tcaggcgata acgcccagtt
    19561 gtttaccaat acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg
    19621 cagacatctg ggtacgaata cgcgcctgac ctttaggaac gaccggatag aagaaaccgg
    19681 tgacgtaaat gccctctttt tgcagctcac gggcaaattt ctgcgccact accgcatcac
    19741 caagcatgac cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca
    19801 tttgctcacg gaactgacgc gcgttcgccc acagacggtc acgcagctcg ctacccgctt
    19861 cgaccatctc cagcactttg atggatgcgg cgacaatcgc tggtgccagc gagttggaga
    19921 acaggtacgg acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat
    19981 aaccaccaga agccccgccc aacgctttac ccaacgtacc ggtaatgatg tctacacgtc
    20041 ccatcacatc gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt
    20101 gggagtcgtc taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca
    20161 ggttggcaat cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg
    20221 caccggcttc acgcgcttct ttcagacgtg cttccagctc ctgcatatcg ttgttggcat
    20281 aacgatagcg tttggcttta cacagacgca caccatcaat aatagacgcg tggttcagtg
    20341 cgtcggagat aatggcgtcc tccgcgctca gcagcgtttc aaacaggccg ccgttagcat
    20401 caaagcagga agagtagaga atcgcatctt ccatccccag gaaggccgcc agtttttgtt
    20461 caagctcttt atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac
    20521 cgtgagaatc cattcccgcc tttgccgccg caatcagatc cggatgattc gccagtccga
    20581 gatagttgtt ggcacaaaag ttaatgacgt ggcttccatc agccacagtg atatctgctt
    20641 gctgcgcaga cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca
    20701 gatcgttggt taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa
    20761 ttccagcaca tattacccaa acttataggt cgcgacgaga tgacgcgtta acacttctgc
    20821 aaaattcagg ataaataacg tccatcaatc tcacttttgg cgaatggctg aagccaacgc
    20881 cgtgtagtga tatgatagaa gcattcgtgt ctgagattgt ctctgactcc ataattcgaa
    20941 ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt atcggcagca acatcgttaa
    21001 agccctgaat gacaaaggca tcaccgatat tctggtggtg gacaacctga aagacggcac
    21061 caagtttgtg aacctggtgg atctggatat cgcggactat atggataagg aagacttcct
    21121 gatccagatt atggctggcg aagagttcgg cgatgtcgaa gcgattttcc acgaaggcgc
    21181 gtgctcttcc accaccgagt gggacggcaa atatatgatg gataacaact atcaatactc
    21241 caaagagctg ctgcactact gtctggagcg cgaaatcccg ttcctgtatg cctcttccgc
    21301 agccacttac ggtggacgca cctccgactt tattgaatcc cgcgaatatg aaaaaccgtt
    21361 gaatgtctac ggttactcaa aattcctgtt tgatgaatat gttcgccaaa tcctgcctga
    21421 agcgaactcg cagattgttg gcttccgtta tttcaacgtt tatggaccgc gtgaaggcca
    21481 taaaggcagt atggcgagcg tcgctttcca tctcaacacc cagcttaata acggtgaatc
    21541 accgaagctg ttcgaaggta gcgagaactt taaacgtgac ttcgtctatg tgggcgacgt
    21601 ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc ggcatcttca acctcggcac
    21661 cggtcgtgcg gaatccttcc aggcagtagc agatgctacg cttgcttatc acaagaaagg
    21721 ccaaatcgaa tacattccgt tcccggataa actgaaaggc cgctaccagg cgttcactca
    21781 ggcagatctg acgaatctgc gcgcggcggg ttacgataaa ccgttcaaaa ccgttgccga
    21841 aggcgtaacg gaatacatgg cctggctgaa tcgtgacgca taagagctct gcatgaaaat
    21901 actggtgatc ggcccgtctt gggttggcga catgatgatg tcgcaaagtc tctatcgcac
    21961 gctccaggcg cgctatcccc aggcgataat cgacgtgatg gcaccggcat ggtgccgtcc
    22021 attattatcg cggatgccgg aagttaacga agcgatccct atgcctctcg gtcacggagc
    22081 gctggaaatc ggcgaacgcc gcaaactggg tcatattctg cgtgaaaagc gctacgaccg
    22141 cgcctacgtc ttacctaact ccttcaaatc cgcattagtg cctttcttcg caggtattcc
    22201 tcatcgcacc ggctggcgcg gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct
    22261 cgataaagaa gcctggccgc taatggtgga acgctatgtc gcgctggcct atgacaaagg
    22321 tattatgcgt accgcacaag atctgccgca gccattgtta tggccgcagt tgcaggtgag
    22381 cgaaggtgaa aaatcatata cctgtaatca attttcgctt tcatcagaac gtccgatgat
    22441 tggtttttgc ccgggtgcgg agtttggtcc ggcaaaacgc tggccacact accactatgc
    22501 ggagctggca aagcagctga ttgatgaagg ttatcaggtg gttctgtttg gctcggcgaa
    22561 agatcatgcg gcgggcaatg agattcttgc cgctttgaat acagagcagc aggcatggtg
    22621 tcggaatctg gcgggagaaa cacagcttga tcaagcggtt atcctgattg cagcctgtaa
    22681 agccattgtt actaacgatt ctggtctgat gcacgttgcg gcggcgctca atcgtccgct
    22741 ggttgccctg tatggaccaa gcagcccgga cttcacacca ccgctatccc ataaagcacg
    22801 cgtgatccgc ctgattaccg gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta
    22861 tcaccagagc ttgatcgaca ttactcccca gcgcgtactg gaagaactca acgcgctatt
    22921 gttacaagag gaagcctgac ggatgcgggt tctgatcgtt aaaacatcgt cgatgggcga
    22981 tgttctccac acgctgcccg cactcactga tgcccagcag gcaatcccag ggattaagtt
    23041 tgactgggtg gtggaagaag ggttcgcaca gattccttcc tggcacgctg ccgttgagcg
    23101 agttattcct gtggcaatac gtcgctggcg taaagcctgg ttctcggctc ccataaaagc
    23161 tgaacgcaaa gcgtttcgtg aagcgctaca agcaaagaac tatgacgcag ttatcgacgc
    23221 ccaggggctg gtaaaaagcg cggcgctggt gacgcgtctg gcgcatggcg taaagcatgg
    23281 aatggactgg caaaccgctc gcgaaccgtt agccagcctg ttttacaatc gtaaacatca
    23341 tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa ctgtttgcca aaagtctggg
    23401 ctatagcaaa ccacaaaccc agggcgatta tgctatcgca cagcatttcc tgacgaacct
    23461 gcctacagat gctggcgaat atgccgtatt tcttcatgca acaacccgag atgataaaca
    23521 ctggccggaa gaacactggc gagaattgat tggtttactg gctgattcag gaatacggat
    23581 taaacttccg tggggcgcgc cgcatgagga agaacgggcg aaacgactgg cggaaggatt
    23641 tgcttatgtt gaagtattgc cgaagatgag tctggaaggc gttgcccgcg tactggccgg
    23701 ggctaaattt gtagtgtcgg tggatacggg gttaagccat ttaacggcgg cactggatag
    23761 acccaatatc acggtttatg gacctaccga tccgggatta attggtgggt atgggaagaa
    23821 tcagatggtt tgtagggctc cggggaatga gttgtctcaa ttgacagcaa atgctgttaa
    23881 gcaattcatt gaagaaaacg ctgaaaaagc tgccatgatt taaattacaa ctctttaata
    23941 gataattttg aaataaaatc agcttccttg ctgatt
//
LOCUS       DAZFRG010000055        23618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFRG010000055 DAZFRG010000000
VERSION     DAZFRG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.055"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..705
                     /locus_tag="ACHKUC_001689"
     CDS             34..705
                     /locus_tag="ACHKUC_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6617958.1"
                     /translation="MCTSFFAQSNMTVYPMAVSINSQGEGSVRVISKSNEVQYIKATV
                     FRIDNPSTPLENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFVAMEPEQQEKNYRVK
                     FEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPVTKLEINSAKKLLNAGNQRL
                     KVLTIAYCKNNSKDNSCKTQTVNKNIFPGQEKSLESISGYDKIVVKYNNWITKDNGEF
                     ELAVH"
     gene            735..1235
                     /locus_tag="ACHKUC_001690"
     CDS             735..1235
                     /locus_tag="ACHKUC_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO6617959.1"
                     /translation="MKKVFVKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NSALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVSWGGEEIKADAATTFSAVKIFASDAITNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1328..4012
                     /locus_tag="ACHKUC_001691"
     CDS             1328..4012
                     /locus_tag="ACHKUC_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO6617960.1"
                     /translation="MDKKLLALLILASLSPAKATLTKIPAGFEVIAQGQQEYIEVYFA
                     GKSLGKYYAMVNLDTVTFLDSSSLYSKLELSTDDQKITHTVKEKLSQPLARHGELACG
                     FVRTDSGCGFLNTETVEIIYNDEESSVTLFLNPQWSSAFNSKSLYLNPDKNTVNAFIH
                     QQDINVLVQDDYQSLSIQGNGALGITDNSYIGAHWNFDGYDADDVSDNNVDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQTQSQQGT
                     PVMILLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGEAGNLAISASVFRNDDGGKGDMQQANWSHPGWPTLGFYRTN
                     SDGDACTTDNRESYNALSCYESISATVSQNLVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTSAQSISETVQLSASRAFVMRDWILSTSLGVFHRNDNGGGSDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSDQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRQNHDHLSAFTGTYSSTLAVSRYGINVGA
                     SGTDDLLGAVLVDVKGFSEQDEQSQDLQLEARVAGSRTLQLGQRDSVLFPYPGFQSGF
                     VEVNDSNQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPASVEK
                     FPLVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVLKKRASIRYSGDV
                     NCSVVSYSQLPESIQVQAQLKQPKLRGNVQTAQREIAP"
     gene            4009..5091
                     /locus_tag="ACHKUC_001692"
     CDS             4009..5091
                     /locus_tag="ACHKUC_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO6617961.1"
                     /translation="MINRLIAVVLCLFGMVAWVQATPNVTAEITYDLAVGRADYYFWN
                     EEESAGNNAYMWYECSYPNLQQTCTNSGNISTVQIYLTEQRSGMRWPLKLKGFKEAYV
                     MNQVPGCEGYVAKTHLQESNRYNCKATGDPTIYYADKKFLTLYLEQAEMKKLPIGGVW
                     KGQVKLHSNLPAQDYFADITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNASNY
                     AQDLTTLDMCLYDGFNGNAISYEIMLKDEGLPAAGRRDGYFSIYRQGRTTTDDVERID
                     YRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKF
                     SVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5190..6488)
                     /gene="exuT"
                     /locus_tag="ACHKUC_001693"
     CDS             complement(5190..6488)
                     /gene="exuT"
                     /locus_tag="ACHKUC_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO6617962.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            6971..8383
                     /gene="uxaC"
                     /locus_tag="ACHKUC_001694"
     CDS             6971..8383
                     /gene="uxaC"
                     /locus_tag="ACHKUC_001694"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO6617963.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8398..9885
                     /gene="uxaA"
                     /locus_tag="ACHKUC_001695"
     CDS             8398..9885
                     /gene="uxaA"
                     /locus_tag="ACHKUC_001695"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO6617964.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9968..10519
                     /gene="ygjV"
                     /locus_tag="ACHKUC_001696"
     CDS             9968..10519
                     /gene="ygjV"
                     /locus_tag="ACHKUC_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO6617965.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(10523..11767)
                     /gene="sstT"
                     /locus_tag="ACHKUC_001697"
     CDS             complement(10523..11767)
                     /gene="sstT"
                     /locus_tag="ACHKUC_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO6617966.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12091..13056)
                     /gene="alx"
                     /locus_tag="ACHKUC_001698"
     CDS             complement(12091..13056)
                     /gene="alx"
                     /locus_tag="ACHKUC_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO6617967.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVE"
     gene            complement(13339..14325)
                     /gene="ygjR"
                     /locus_tag="ACHKUC_001699"
     CDS             complement(13339..14325)
                     /gene="ygjR"
                     /locus_tag="ACHKUC_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6617968.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGALVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            complement(14404..15096)
                     /gene="ygjQ"
                     /locus_tag="ACHKUC_001700"
     CDS             complement(14404..15096)
                     /gene="ygjQ"
                     /locus_tag="ACHKUC_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO6617969.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            complement(15173..15676)
                     /gene="ygjP"
                     /locus_tag="ACHKUC_001701"
     CDS             complement(15173..15676)
                     /gene="ygjP"
                     /locus_tag="ACHKUC_001701"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO6617970.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15761..16897
                     /gene="rlmG"
                     /locus_tag="ACHKUC_001702"
     CDS             15761..16897
                     /gene="rlmG"
                     /locus_tag="ACHKUC_001702"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO6617971.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17182..17289
                     /gene="higB"
                     /locus_tag="ACHKUC_001703"
                     /pseudo
     CDS             17182..17289
                     /gene="higB"
                     /locus_tag="ACHKUC_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
     gene            17299..17715
                     /gene="higA"
                     /locus_tag="ACHKUC_001704"
     CDS             17299..17715
                     /gene="higA"
                     /locus_tag="ACHKUC_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO6617972.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17808..19826)
                     /gene="fadH"
                     /locus_tag="ACHKUC_001705"
     CDS             complement(17808..19826)
                     /gene="fadH"
                     /locus_tag="ACHKUC_001705"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO6617973.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVGAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGEPQILAVDHVVIC
                     AGQEPNRTLAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19997..22348)
                     /gene="ygjK"
                     /locus_tag="ACHKUC_001706"
     CDS             complement(19997..22348)
                     /gene="ygjK"
                     /locus_tag="ACHKUC_001706"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO6617974.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVIFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKNWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTENGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFQHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22365..23435)
                     /gene="ygjJ"
                     /locus_tag="ACHKUC_001707"
     CDS             complement(22365..23435)
                     /gene="ygjJ"
                     /locus_tag="ACHKUC_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO6617975.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLTEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
BASE COUNT         5940 a         5999 c         6027 g         5652 t
ORIGIN      
        1 aatcgattat tacggctcgg gcctgtctta cttctgtgta catcattttt cgcacaatct
       61 aatatgactg tctaccctat ggctgtttcc attaatagcc agggagaagg aagtgttcgt
      121 gtaatatcta aaagtaacga agttcaatac ataaaagcga cagtattccg catcgataac
      181 ccatcaacgc ctctagaaaa cgaagtggaa attaaatccg gtgatgctaa tcatttggtt
      241 gttatgccac ctaaattcgc cttaccagcc ggtagtagca aaaccgtgcg ctttgtcgca
      301 atggaaccag agcaacaaga aaaaaactat cgtgttaagt ttgaagctgt acccagcatt
      361 gatgatgttg ctaccgataa aaaagatctc tctatgcagt taacggttaa tttaatttgg
      421 ggcatcgttg ttagtgttcc acctcaacaa ccagttacta aattagaaat taattcagca
      481 aaaaaactac tcaatgcagg aaatcagcgt cttaaagtgt tgacaatcgc ttattgtaaa
      541 aataatagca aagataatag ttgtaagact cagaccgtaa ataaaaacat tttccctggt
      601 caggaaaaaa gtttagaaag catatctggc tacgataaaa tcgttgtcaa atataataac
      661 tggatcacca aggacaatgg cgagtttgag ctggcggtcc attaattatc gttgtgataa
      721 acaaaggaat agtcatgaaa aaggtattcg taaaatctct tctggtagcc gcaatgtttt
      781 ccgttgcagg ttctgcatta gcagtacaaa aagatattac tgtcactgct aatgttgatg
      841 ctgctctgga tatgactcag accgataact ctgcgctgcc gaaagcagta gagatgcaat
      901 atctgccggg tcagggtctg cagtcttatc agttaatgac caaaatctgg tctaacgacg
      961 tcactaaaga tgtgaaaatg cagttagttt ctccggcgca actggttcag agcctggacg
     1021 ccagcaaaat tgttccgctg accgtaagct ggggtggtga agagattaaa gctgatgcag
     1081 caaccacttt tagcgcagtt aaaatttttg cgtctgatgc tataaccaac ggttctctgg
     1141 ctaagaacct gatgtttgct cagaccacca aaggtgttct ggaaacaggt atctaccgtg
     1201 gcgtagtcag catttatctg tctcaggata tctaatctgg atatctgaat taacaggtaa
     1261 taaggggggc aagtcgtcct cctttatttt aactattctg gtatttatta tggataacag
     1321 gactgccatg gataaaaaat tactggctct tttgatcctg gcgagtctca gcccggcaaa
     1381 ggcgacatta accaaaattc ccgctggttt tgaggttatt gctcagggac agcaggagta
     1441 tatcgaggtt tattttgcag ggaaaagtct cggtaaatat tatgcgatgg ttaatcttga
     1501 taccgtaacc tttcttgatt catcaagttt atacagcaaa cttgagttga gcactgacga
     1561 tcaaaaaatc actcatacag tgaaagaaaa actatcgcag cccttagctc gccacggtga
     1621 actggcttgt ggttttgtac gtactgattc aggatgcggt tttcttaata ctgagacagt
     1681 agaaataatc tacaatgatg aagaaagttc agtaacgcta tttcttaatc cgcaatggag
     1741 ttctgcgttt aactcgaagt cattgtattt aaatccagac aaaaatacgg ttaacgcttt
     1801 tatacatcag caagacatta atgttctggt acaagatgat taccaatcgt tgtcaattca
     1861 gggtaatggc gcactgggaa taacagataa tagctatatt ggtgcgcact ggaatttcga
     1921 cggttatgat gcagatgatg tcagcgacaa caatgtcgat gtcagcgatc tctattaccg
     1981 ctatgatttt ttacgccgtt attatgtgca ggcggggcgg atggacaacc gcacattatt
     2041 taatgcgcaa ggtggaaact ttacttttaa ctttctgccg cttggtgcaa tcgacggtat
     2101 gcgtatcggg tcgacactca gttatttaaa ccagacgcaa agccagcagg gaacgccggt
     2161 aatgatcctg ctttcgcgca attctcgtgt tgatgcttat cgtaatgaac aactgttggg
     2221 atcgttttac ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgccaggcag
     2281 ctacagcgtg gcgttaaaag tttatgaaaa taaccaactc acccgcaccg agcttgtgcc
     2341 gtttaccaaa actggcggtc tgacagacgg aaatgcccag tggttcttac aggcaggtaa
     2401 aaccacatca caggtttctg atgatgaaag ttcagcttat cagctagggg tacgcctgcc
     2461 attacatccg caatatgagc tctacgcagg gctggcgaat gccgatgatg taagtgcttt
     2521 cgagttaggt aataactgga cggcggattt aggcgaggcg gggaatcttg ctatcagtgc
     2581 cagcgtgttc cgtaacgatg acggcggcaa aggcgatatg caacaggcca actggagtca
     2641 tccgggatgg ccgacgttgg gcttttatcg gaccaactct gacggtgatg cttgtacaac
     2701 cgacaacaga gagagctaca acgccttaag ctgttatgaa agtatttccg cgacggtttc
     2761 acagaatctt gtcggctgga atatgatgct gggttatacc cgcacacaaa ataacactga
     2821 tgatagttta cgctgggaca aacagcagag ctttgaaaat aactatcttc gccagacttc
     2881 ggctcaaagc atttccgaga ccgtacaact tagcgcttct cgcgcttttg tgatgcgtga
     2941 ctggattttg agtacttccc ttggggtttt ccatcgtaat gacaacggcg gtggtagcga
     3001 tgacaacggt ttatatctgt cgttttcgtt atctgatacg ccgacgatgg acagcaataa
     3061 caacagccat tcaaccaatg tttctactga ttatcgttat agcgatcagg atggcgatca
     3121 aacgtcatgg cagttatcgc atacctttta taacgactca ttcagccata aagagcttgg
     3181 cgtgaccgtc ggcggtttga acaccgatac cataaacagc gcggttaacg ggcgttggga
     3241 tggtcaatat ggaaatgtct acgctacagt ctcagacagt tatgatcgcc agaatcatga
     3301 tcatctctcg gccttcaccg ggacatacag ctccacgctg gcggtgagtc gctatggcat
     3361 caatgtgggg gccagcggca cagatgattt gctgggggcg gtgttggtgg atgtgaaagg
     3421 tttctctgaa caggatgaac agagtcagga tctgcaactc gaagcgcggg tggctggcag
     3481 cagaacattg cagcttggtc aacgcgacag cgtgttattc ccctatcctg gatttcagtc
     3541 tggctttgtt gaagttaacg acagtaatca gggtaaccag caaggaacaa ccaacatcat
     3601 taacggtgcg ggaaatcgtg agttaatgct gttgccgggc aaattacgtt atcgcgaggt
     3661 gtcggccagt tttaattaca actatattgg tcgcttgtta ttacccgcat cggtagagaa
     3721 gttcccgctg gtggggctga atagcgccat gctactggtg gctgaagatg gcggatttac
     3781 actggagatt aacggtagtg aaaaagaact gtatctgctt tccgggcagc agttcctgaa
     3841 atgtccgctg agtgttttga agaaacgcgc cagcattcgc tatagcgggg atgtgaattg
     3901 tagcgtggtg agttattcgc aactgccgga gtctattcag gttcaggcac agttaaaaca
     3961 gcctaaatta cgtggaaacg tccagacggc gcaaagggag attgcaccat gataaatcga
     4021 ttgattgccg tggttttatg cttatttggc atggtcgcgt gggttcaggc aacacccaat
     4081 gtgacggctg aaattactta tgatttggcc gtcggtaggg ctgattatta cttctggaac
     4141 gaggaagagt cggctgggaa taatgcatat atgtggtatg aatgttcgta ccccaatctc
     4201 cagcaaacct gtacgaattc tggaaatatc tcgacagtac aaatttattt aacggagcaa
     4261 cgtagtggta tgcgctggcc acttaagctc aaaggattta aagaagctta tgtcatgaat
     4321 caagtacctg ggtgcgaagg atatgttgca aaaacgcacc tccaggaatc taatcggtat
     4381 aactgcaagg caactggtga tccaaccata tattatgctg ataaaaagtt tcttacactt
     4441 taccttgaac aggcagaaat gaagaagctg ccaattggtg gtgtctggaa agggcaggtt
     4501 aaactgcatt ctaatcttcc tgctcaggac tattttgcgg atattaccct gaacaccctc
     4561 gatcccaatc atattgacgt attcttcccg gagttcgccc acgctacgcc acgggtacaa
     4621 ctggatttgc acccaacagg cagcgtgaat gccagtaatt atgcccagga tctgacaacg
     4681 ttggatatgt gcctgtacga cggttttaac ggtaatgcca tcagttatga aattatgctt
     4741 aaagatgaag ggctacccgc ggcagggcgc agagatggtt atttttccat ttatcgtcag
     4801 ggaagaacaa cgactgacga tgtagagcgt atcgattaca gagtaaaaat gtataacccg
     4861 gaaacaggtg ggcaaatcga cgtacgcaat aatgaaaata tggtctggaa cagcatcaac
     4921 ctgaagcgag ttcgtccggt ggtattgccg ggcattcgtt acgccgtaat gtgtgtgcct
     4981 acgccactaa ccctggcggt cgataaattc agcgtgatgg ataaacaggc gggatattac
     5041 atgggtaaat tgtcggtcat ttttaccccg tcattgccga cgatcaatta gctctgtagg
     5101 ccagacagac gttttcgcgt cgtctccggt aataaaaacg ctgaagccgc ctgaaaaggc
     5161 ggcttcgtca ttacaactta cattaagtgt taatgttgcg gtgcgggatc gttatgtgat
     5221 tcctgcgcca cctcaatcgc cggtttgttc tgcaacacgg tccagataac cagcgcacct
     5281 aacaggtcga acactgccag aactgcgaac agcgggctga agccgatggt gtcagccagt
     5341 gcaccgacta ccagtgcaaa cagcgtactt gccagccatg cggacatccc ggttaaaccg
     5401 ttcgccgtcg ccacttcgtt acgaccgaac acgtcagacg agagcgtaat cagtgcgcca
     5461 gacagtgcct ggtgagcaaa accaccgata cacagcagca taatcgcgac atacgggttg
     5521 gtgaacagac cgatcatgcc cgggccaatc atcagcacag cgcccagcgt tacgaccatc
     5581 ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg caggtaacca
     5641 ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcaaacat cgcgatctct
     5701 ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt aaaagtaccc
     5761 catgccggtt ctgccaggaa gcgcggcagc gcgatacccc agaactgacg gttgcgcagg
     5821 atctgaccaa cggacatttt cttcgctgtg tcaacctggt gctgggcttc ctgaccatta
     5881 ataatgtagt cgcgttcttc atcggtcaga tgtttctggt cgcgcggatg cttgtagaaa
     5941 atcagccatg ccatcgccca gataaagctc aacgcaccgg agatgataaa tgccatctgc
     6001 cagctgtgca tcacgattgc ccataccacc agcggcggcg caatcatcgc accaatcgaa
     6061 gaacctacgt taaagtaacc taccgcgatg gaacgctctt tcgccgggaa ccattcggag
     6121 ctggctttca gacccgccgg aatcatcgcg gcttccgcgg caccgaccgc accacgagca
     6181 accgccaggc caccccaact acctgccagc gcagttgcac cacagaacac ggcccacagt
     6241 acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata acccgctacc
     6301 ggttgcatga ccgtataagc agcagaatac gctgcgatga tataggaata ctgttgggtg
     6361 gagatgttta actcttccat cagagttggc gcagctgccg ccacagtgtt acgcgtcagg
     6421 taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa ccctttaatt
     6481 ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc atcatcccgc
     6541 cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaccggca cgccccgaac
     6601 gttgccatga aagtttcgtg ctaattcggc ttccgtaccg gttaacccga ttcatctaac
     6661 aaatgaatga atcggcaatt tgcattccgt cgacttatga tttgcgacgg cagaaagata
     6721 acttgtcata caactttaaa aggtgagagc catcacaaat gtgcgaatat ttgtagggac
     6781 attacctgac gacggcgagg ccagtgctgg cgcggcctgc agcgagattt accactttga
     6841 gagtaatttt tttaactacg tttattgatc taactcacga aaatatcttc ggactctgga
     6901 aattggtgtg ataactttgt cagcatcgca ccataagcaa gctagctcac tcgttgagag
     6961 gaagacgaaa atgactccgt ttatgactga agatttcctg ttagatactg aatttgcccg
     7021 ccgtctgtat cacgactacg caaaagacca gccgattttc gattaccatt gccatttgcc
     7081 gccgcagcag attgcggaag actatcgttt taaaaacctg tatgacatct ggttgaaggg
     7141 cgatcactac aaatggcgcg ctatgcgtac caacggtgtg gccgagcgtc tgtgcaccgg
     7201 tgatgcgtct gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc acactattgg
     7261 caacccgtta taccactgga cgcacctcga actgcgtcgt ccgtttggta tcactggcaa
     7321 attgctttct ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca
     7381 ggataatttc tctgcacgcg gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac
     7441 cgatgacccg atcgattctc tggagcatca cgcagagatc gccaaagacg gctctttcac
     7501 cattaaagtg ctgccgagct ggcgtccgga caaagccttt aacatcgaac aggcgacctt
     7561 taacgactac atggcgaagc tgggtgaagt ctctgatacc gacatccgcc gctttgctga
     7621 tctgcaaact gccctgacca aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc
     7681 tgaccacgcg ctggatgtgg tgatgtttgc tgaagcgaac gaagcggaac tggacagcat
     7741 cctcgcgcgc cgtctggcag gcgaaaccct gagcgagcac gaagtggcac agttcaaaac
     7801 tgcggtgctg gtgttccttg gtgccgaata cgcacgtcgc ggctgggtac agcagtacca
     7861 cattggcgca ctgcgtaata acaacctgcg tcagttcaaa ctgctggggc cggatgtagg
     7921 ttttgattcc atcaacgacc gtccgatggc ggaagagctg tctaagttgc tgagcaagca
     7981 gaacgaagaa aacctgctgc cgaaaaccat tctctactgc ctgaacccgc gcgataacga
     8041 agtgctgggc accatgatcg gtaacttcca gggcgaaggt atgccgggca aaatgcagtt
     8101 cggttccggc tggtggttta acgatcagaa agacggtatg gaacgtcaga tgacccaact
     8161 ggcgcagctc ggtctgctga gccgctttgt cggtatgctg actgacagcc gtagcttcct
     8221 gtcatacacc cgtcacgaat acttccgccg cattctgtgc cagatgattg gtcgctgggt
     8281 ggaagcgggc gaagcgccgg cggatatcaa cctgctgggc gagatggtga aaaatatttg
     8341 ctttaacaat gcgcgtgact acttcgccat tgaactgaac taaggtctgg ggttgatatg
     8401 caatacatca agatccatgc gctggataac gtcgcggttg ctttagcgga tttggctgaa
     8461 ggcacagaag tcagtgtcga taaccagact gttacgctgc gccaggatgt tgctcgtgga
     8521 cataaatttg cgttaacgga tatcgcaaaa ggggccaacg tcattaaata tggcctgccg
     8581 attggttatg cattggcgga tattgcggcg ggggaacacg ttcacgccca caatacgcgc
     8641 acgaatctga gcgatctgga tcagtatcgc tatcaacctg attttcagga tctgcctgcg
     8701 caagcggcag atcgtgaagt gcagatctat cgtcgcgcta acggcgatgt cggggtgcgc
     8761 aatgagctgt ggatcctgcc aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccaa
     8821 aatcgtttcc tgaaagagac caacaacgct gaaggtaccg acggcgtgtt cctcttcagc
     8881 cacacctacg gctgctcaca actgggcgac gatcacatca atactcgcac catgctgcaa
     8941 aacatggtgc gccacccgaa cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac
     9001 aaccaggttg ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc
     9061 atgatctgcc aacagcagga tgatgaaatc gaggccggga tcgcgcattt gcatcagctg
     9121 tatagcgtga tgcgcaacga taaacgcgag ccgggcaaac tcagcgaact gaagtttggt
     9181 ctggagtgcg gtggttctga cggtctttct ggtattactg ctaacccgat gctggggcgt
     9241 ttctctgact acgtgattgc taacggcggt actaccgtac tgaccgaagt gccggagatg
     9301 tttggcgcag agcagttgct gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg
     9361 gtcactatgg tcaacgactt caaacagtac tttattgccc atgaccagcc gatctatgaa
     9421 aatccatcac cggggaacaa agcgggcggt atcaccacgc tggaagacaa atcacttggc
     9481 tgtacccaga aagcgggttc cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg
     9541 aaaacgccgg ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc
     9601 ctggcgggtg cgggctgcca tatggtgctg ttcagtactg gtcgtggcac gccgtatggt
     9661 ggatttgtgc cgacggtgaa aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac
     9721 tggatcgact ttgacgcggg tcagctgatc cacggtaaag cgatgccgca gttgctggaa
     9781 gaatttatcg acaccatcgt tgagtttgcc aatggtaaac aaacctgcaa cgagcgtaac
     9841 gacttccgcg aactggcgat cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg
     9901 ttaataaaaa acggcgtttc ataatgtgaa gcgccgtttg cttttccata cctgctaagg
     9961 atttatcatg accgcgtatt ggctggccca gggcgtgggt gtcatcgcct ttctgattgg
    10021 tatcacgaca tttttcaacc gtgacgaacg tcgcttcaaa aagcaacttt cggtctatag
    10081 cgccgttatt ggcgtacatt tttttcttct gggcacctat cctgccggtg ccagtgccat
    10141 ccttaatgcc attcgtacat tgattacctt acgcacgcgc agcttatggg taatggcgat
    10201 ttttattgtg ctgactggcg gaattggcct cgcgaagttc catcatcctg tcgaactatt
    10261 gccggtaatc ggcacgattg tcagtacctg ggcgctgttc cgctgtaaag ggctgaccat
    10321 gcgctgcgta atgtggtttt caacgtgttg ctgggtgatt cacaacttct gggcggggtc
    10381 gataggcggc acgatgattg aaggcagttt tctgctgatg aatggcctga atatcattcg
    10441 tttctggcgt atgcagagaa ggggaattga tccgtttaaa gtggagaaaa ccccttccgc
    10501 cgtagacgaa aggggctaag tattagttac gcagggcgct atttgccaga cgatcgtctt
    10561 ccgcctggca agctgccgca gtgaacagca cgtcagttga cgagttcagc gcggtttcac
    10621 aggagtcctg caatacgccg ataataaagc cgacagcaac cacctgcatg gcgatatcgt
    10681 tcgaaatacc gaacatatta caggccagtg ggatcagcaa cagagacccc cccgccacgc
    10741 cggatgcgcc acaggcacac agagaagcca ctacgctcaa cagcagcgct gtgggcagat
    10801 caaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
    10861 cgcccgccat attgatggtg gctcccagcg gaatagaaac ggaataggta tcgcgatcca
    10921 gattcagctt ttcacacagc gccatattca ccggaatgtt cgccgcagag ctgcgggtga
    10981 agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
    11041 gaattttcca ccataccagc agtgggttaa ccaccagcgc caccagcagc atacagccaa
    11101 ccagtaccac cagcagttgc gcgtagcccc acagcgtgga gaaaccggtg gttgccaggg
    11161 tagaagaaac cagaccaaaa ataccgatcg gtgcgaagtg aatcaccagt ttcaccataa
    11221 aggtaacggc attcgacata tcgttgacca ggtttttggt ggtttcgtta ccgtgacgca
    11281 gtgcgaaacc gaggccaatt gcccacacca ggatcccgat atagttacct ttcagcagcg
    11341 cgtcaatggg gttggaaacc atgctcatta ccagcccgcg catcacttct acaatgcctg
    11401 acggcggcga aatatcaccc gcgctactgg acaagtgcag ggtagaaggg aaggcaaaac
    11461 tgaagattac tgcggccaga gcagcagaga aggtgcccag cagatagagg aacaaaatag
    11521 ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatt accagcatca
    11581 acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa
    11641 cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca
    11701 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
    11761 tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc
    11821 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
    11881 gaagcgaaat atgctggatt ttaaagagaa gtcatatttc gcaacttaat ggttacatct
    11941 gtttgacatg aaaataaagc ccaacgtgcg gcttccagac tgatgacaac gtcaaaaaag
    12001 tactcggtca gtcccctcgc cccttttggg gagagggaga aaacggtgct catgttgatg
    12061 acagagtaca cggcttaggc agattaaaaa ttattctacc cgctgcttat catgccgata
    12121 attcacccag gcgttgataa taaacgtcat caccagaatg ccaaacacca cgcccagcga
    12181 gacagcgatc ggaatatggt agaagtcgac aatcagcatc ttgataccga taaacaccag
    12241 aatcaccgcc agaccatatt tgagcatcga gaaacgctct gccacacccg ccagcaggaa
    12301 atacatcgca cgcaggccga ggatcgcaaa caggtttgag gtcagcacaa taaacggatc
    12361 ggtggtcacg gcgaagatag ccggaatact atccacggcg aaaatcacgt cgctcaactc
    12421 caccagaatc agcaccagca tcagcggcgt ggcatacaac aaaccattct tacgcacaaa
    12481 gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag
    12541 cggcttgtcg ccaatgcctg attcatcttc atgggcgagc gccatcttca cgccggtaaa
    12601 cagcaggaag gcaccgaaga tatacaagat ccagtcgaac tgtgaaatca gccagctacc
    12661 agtgaagatc atgatggtac gcagaacaat cgccccgagt acgccataca ccagcacgcg
    12721 gcgttgtaat gccgccggaa cagagaaata gctgaacaac atcagccaga caaagacgtt
    12781 atcgaccgcc agcgatttct caatcagata acccgtgaga aaggccagtg cctgtggatc
    12841 ggcaacggcg cgaccttcgg tttgcaccag ataccaccag aaagcggcgt taaacagtaa
    12901 cgacagcgtc acccagacca gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc
    12961 cccacgacgc ccctgcaaca acaggtcgat agccagcata atggtgacaa caacagcgaa
    13021 tccgccccat agcaacggcg tgccgacagt attcatagaa gttccttaca cataaaaaaa
    13081 cggccaacgt cggaagacgt cagccgctgc tttttatgca tagacctcgc cttccggcaa
    13141 ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac
    13201 cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg caatgaagtt actccccttt
    13261 gcaggtaaca aagtatgaca gaccattcag tcagtcaatg gcctgttaca taccttttac
    13321 actgttttac tttggcaatt atagttttac gttatctgcc ggaaaaatca ccccagtctg
    13381 gcggcggatc tcagtcagca gtttggcggt gatacgactg accgccagcc ctggatgatt
    13441 caccagatgc tcatccacca gggtagcgaa cagctctgct tcgtagagca tggtattaat
    13501 atgctgtggc tgggtgagat cctgcatctg gctgccacgc ggtacaaagc agactttctg
    13561 gcattcagag agtttttcaa tcaccagcgc tcccgcttcg ccctgaatct cgcttgccag
    13621 gacagaatcg ctcacttttg agtgctgcaa cgtaacgctg aaatcaccgt aatccatcac
    13681 caccacgccg tgggcatcca caccacttgc cagcaaactg gcggttgcct gcacgctttt
    13741 cggttcaccg aataatgcca ccgccgacgc caggcagtaa aagccgatat ccataatcga
    13801 accgttggag aatgccggat tgaaggtgtt gggattctct ccgtccaggt aacgctgata
    13861 ccgcgaggaa tactggcaat agttgaaaaa gactttacgc agtttgccga ctttcggcag
    13921 cgcctggcgt aacagatgaa agttcggcag gtaggccgtt ttaaatgcct caaacagcac
    13981 cacctgattt tcccgcgcac aggcaatggc ggcatccact tccgccagat tcgacgccag
    14041 cggtttctcg caaatcacat gaattttgtg actaaggaaa agttgtgtct gggaaaagtg
    14101 cagggaattc gggctggcaa tatacaccgc gtcaatggca tcgctttctg ccatcgcttc
    14161 cagcgacgta aacagatgct cgacagaaaa atcattggcg aagtgctggg cctgttcaag
    14221 actgcgggaa taaacggcgg ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa
    14281 ctggcgagtg atccagttcg taccaatcac agcgaaacgt atcataacgc gtggatactc
    14341 cataaaggga ttctctcgtc actgtagcac gcctttttta tcttcgacgt gcgcagatac
    14401 gcattattct tttgccggac agccatgctc actaaacggc ccaatcataa ccaacggccc
    14461 gagatatttc ggctggcgat ggaggatttt cgcatcaatc accgcgctga ctctggcaag
    14521 tttttcccgc agttgagtat ataaaccgtg cttcatattc aggtctggcg cgttaaaacc
    14581 gatagcatcg ataccatact gttttgccag ccagatggcg cgttggttat gaaattcttg
    14641 cgagataatg gtgatatggt tttcactaaa caccttgttg gcacgcacca ccgaatcaag
    14701 cgttgagaat ccggcatagt cgcagaagat ggcttttgcc ggtacgcctt tagcgatcaa
    14761 ggcttgctgc attcctgacg cttcatcgta atttttacgt ccattatcgc cgctcaccaa
    14821 tagccatttc acttttccgg cgtggtacaa ctctgctgcg gtatcaatac gtcgggtaaa
    14881 gtagcgatta cccggcctcg cccccagcaa taaacccacg ttacgcgccg gaacggcgtt
    14941 gacatcgctc caggtcagtt gttggctggc gttgaccata acgcgatcgg caataaaaag
    15001 ggttgctcct gctataagca acaataaaca gccaatttta agagtccggc gagaaaaaca
    15061 aatacggaga agaaggcggg caaatgcgcg cagcatggtc gttatcctta acgaaaggga
    15121 ggaggtcgat tttaggcgct aaaggaggaa aaaataacgc ctgacatcat tttcagattt
    15181 tgtcctgccc aagcgacaac tgcgttagcc acagccgggt gtcgaactca agctggtggt
    15241 actgcggttc catatgacag cacaactggt aaaacgcttt gttatgctct ttctccttca
    15301 ggtgcgccaa ctcgtgcacc acgatcatgc gcaaaaacgg ttccggcgcg ttgcgaaaca
    15361 cggtagcgac gcggatctct gcttttgctt tcagcttgcc gccctgcaca cgcgaaacag
    15421 cggtatgtaa cccgagcgca tttttcagca catggatctt gttgtcatac atcactttag
    15481 tgatcggcgg ggcattgcgc agaaactgat ttttaagatc ctgagtatat tgccagaggg
    15541 ctttatcggt ggcgtagtcg tgtgttcccg gatagcgttt tgccagcaca tcgcccagac
    15601 gctgttcgtt aatcagcgtt cgcacctggg aaagtagctg ctcgggataa ccctgaagat
    15661 aagtaagatt gctcataacc gcccacgaaa aagagaaaaa gggtatactc acgcaccctt
    15721 ttcaggggca acgtcgaaat tttatcattc aggagggccg atgagccact tagacaacgg
    15781 tttccgttca ctgacactac aacgttttcc ggcgacggat gacgttaacc cgctacaggc
    15841 gtgggaagcg gcggatgaat atttgctgca acagttggac gacacagaaa tccgcggccc
    15901 ggtgttgatc ctgaatgatg cttttggtgc gttaagctgt gtgctggcgg aacataagcc
    15961 ctacagcatt ggtgactcat acatcagcga gctggcgacg cgtgagaatt tacgtctcaa
    16021 cggcattgat gagtcgagcg tgaagttcct cgacagcacc gccgactacc cgcaacagcc
    16081 gggtgtggtg ctgatcaaag tgccgaaaac actggcattg ctggaacaac aactgcgcgc
    16141 gctgcgcaaa gtggtcacgc cagatacgcg cattattgcc ggtgccaaag cccgtgacat
    16201 tcacacttcc acgctggaat tgttcgaaaa agtgctcggt ccgaccacca ccacgctggc
    16261 atggaagaaa gcgcgcctga ttaactgcac cttcaacgaa ccgccgttgg ccgatgcgcc
    16321 gcagaccgtt agctggaagc tggaaggcac tgactggact atccacaacc atgcgaatgt
    16381 tttctcccgc actgggctgg atattggcgc gcgcttcttt atgcaacatc tgccagagaa
    16441 tctcgaaggt gagattgtcg atctcggttg cggtaatggc gttattggcc tgacattgct
    16501 cgataaaaac ccgcaggcga aagtggtgtt tgtcgatgaa tcaccgatgg cggttgcttc
    16561 cagccgtttg aacgttgaaa ccaacatgcc cgaggcgttg gatcgctgcg agtttatgat
    16621 caacaacgcg ctctccggcg tggagccttt ccgttttaat gctgtgctct gcaacccgcc
    16681 gtttcatcag caacatgcgc tgaccgataa cgtcgcctgg gagatgttcc accatgcccg
    16741 ccgctgcctg aaaatcaacg gcgagctgta tatcgttgcc aaccgtcacc tggattactt
    16801 ccataaactg aagaagattt tcggcaactg caccaccatc gccacgaata ataaatttgt
    16861 ggtgctgaaa gcagtgaagc tggggcgtcg tcggtaagat tggctgctac ctgccagata
    16921 aggcgtaaac gccttatctg gcatacgttc agcacattac ccccaggtcg gataagacgc
    16981 ggcaagcgtc gcatccggca atgtctgccc agttctggaa tcgcgcttcc ataaaacaaa
    17041 aaatcccttt caccatcctc tacattctct tgtttagcat ttttctacgt ttattcttcc
    17101 gtcacacaga taaattcatt cgttgccaat ctgtcaacgg atgttatcat attgccaatt
    17161 tgtcaacgga cgtgatgaag aatgcacctg ataactcaaa aagcattgaa agatgctgcg
    17221 gaaaaatacc cgcaacataa aacggagttg gtggctctgg ggaacacgat tgctaaggga
    17281 tatttcaaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagtta
    17341 actgctgtgg caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg
    17401 gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt
    17461 gccaaaataa ccgcgtggga agaatcagcg cccgaatttg ctgaatttaa tgccatggcg
    17521 caagccatgc ctggcggtat agctgtgatt cgtaccctta tggatcaata tggtttaacc
    17581 ctttccgatc ttccggaaat tggcagtaaa tcgatggtgt cacgcgtttt gagcgggaag
    17641 aggaaattaa cgctggaaca cgctaaaaaa ctggcaacgc gattcggcat ttctcccgcc
    17701 ttgtttattg attaacgcga cgtgccggat gcggcgtacg attagcacgt tacccccagg
    17761 ccggataaga cgcgttaagc ggcgcattcg gcacttcaat gcacaattta aatctccagc
    17821 gccagccgtg ttccctgggc aattgcccgt cgtgcgtcca gctccatagc tacatcgcag
    17881 ccgccaatta aatgcacagt tttcccgcta tcaatgagtg gttgcgccag cgtgcggttt
    17941 ggctcctgcc ccgcgcagat caccacatga tctaccgcca aaatttgcgg ttcgccgttg
    18001 atcactacat gcagcccgtc atcgtcgatc ttctggtaac ttacgcctgg gatcattttc
    18061 acgccgcgtg agagcagggt ggtgcgatgg atccagccgg tggttttgcc taacccttgt
    18121 cctggtttgc tggctttgcg ctggagcatc acaatctgcc gtgggctacg ggggatttgc
    18181 atgccttgcg gactcaaacc tccggcctgt tgcaggctac tgtcgattcc ccactcatta
    18241 cagaacccgg cgatattctg gctggtggat tctcccggct gacttaaata catcgccgta
    18301 tcaaaaccaa ttccgccaca accgatgatg gcaactttgt tgcccaccgg cgctttgtcg
    18361 cgcagtacat cgagataact caataccttc ggatgatcga tcccgtcgat aggcggaacg
    18421 cgcggcacga tcccactggc gaggatcgtt tcatcgaacg cctgtaactg atccgctgtc
    18481 acggtgtgat tgagttttag cgtcacaccc gtcacttcga tcatccggcg gtaatagcgc
    18541 agcgtttcgt aaaactcctc tttgccgggg atctgtttgg cgatattaaa ctgcccgccg
    18601 atctcgctgt gcgcatcaaa caatgttacc tgatgcccac gcgccgccgc gttaatggca
    18661 aacgccagcc cagcaggtcc cgcaccgacc accgccagat tttttttctg cacggcagga
    18721 aggattggca ttttggtttc atggcaggcg cgaggattca ccaggcacga ggtgactttg
    18781 ccaacgaaga tctgatcgag acaggcctgg ttgcagccaa tacaagtgtt gatctcatcg
    18841 gctcgtcccg attgcgcttt tgacagcagc tccgcatcag cgagaaacgg tcgcgccatc
    18901 gataccatgt ccgcatcacc gcgcgagaga atgtcgtcgg caacctgcgg atcgttaatc
    18961 cggttggtgg tcaccagtgg cagcgaaacg tggcctttca gtttgcgcgt gacccagcta
    19021 aatgcgccgc gcggcacggg cgtggcaatg gtcggaatac gtgcttcatg ccagccaatg
    19081 ccggtgttga taatcgttgc gcccgccgct tcaatggcct gcgccagctc taccgtttcg
    19141 gcaaaagtac cgccgccttc gaccaggtcg agcatcgaca aacggtagat aataatgaag
    19201 tcgttgccga cacgttcgcg caccgcaccc actacttcta cggcaaaccg catccggttg
    19261 cggtaatcgc cgccccactg gtcactacgc tgattggtgc gcagcgtcag aaattcgttg
    19321 atcaaatacc cttcggaccc catcacctct acaccgtcgt atcctgcctc ccgtgccaat
    19381 tgcgcgcagt gggcgaaatc gtcgatcagt tgcaggatct cttcatgggt gagttcgtga
    19441 ggaacgaaac ggttgatggg ggcctgcaat gcggagggtg cgaccagatg cggttggtag
    19501 ctgtagcgcc cggtatgcaa aatttgcagg gcgattttgc cgccttcctg atgtacggct
    19561 tcggtaatgg tgcgatggtg tgggatctgg ttggcgtcat tgagcattgc accgccttcc
    19621 atgccaacgc ctgttaaatc tggtgcgata ccgccgctga caatcaacgc cacgccgtga
    19681 cgggcgcgtt cggcataaaa cgctgccagc cgttcagcgc cgtccgggta ttcctccagc
    19741 ccggtgtgca ttgagcccat caacacgcgg ttttttaacg tggtaaaacc taaatccagc
    19801 ggggcgaaca gcgacgggta gctcataaaa ttgtccagta tgtaaaataa ttgttatgtg
    19861 gtcggatgag ttctaattta gccgtcgccg gagaaaaggg aaaaggggag tgcggggttt
    19921 gtgatgggat tcaaaaagaa tacgcccgtt cgtcatgccg gacggcggta atacagtcgt
    19981 ccggcatcgg gaaaaattac tgcttacgga aaaaatcgtt atacaacata tacaaatgcg
    20041 cggcactcca ggagaaattt ggtgcgcctt gctgtgcgcc agtcagcggg ttgtaattct
    20101 cctgaattgg gccatcggcg gttaaccctt tggcatgctg gaagaacgta tccgccagtt
    20161 tcagggcatc atcgcgataa ccgtaacgct ccattccttt cagaccaaac cagaactgat
    20221 ctacccatac gcgcccgcgc cagtagatat cagcgccaaa agccgggttg gttaacgccg
    20281 ccgttcccag cgggacaaag gtgttgaact cttttggatc gagcatcacc ttcaccaccg
    20341 cgtcggcgtt ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct tctggccctt
    20401 taccgcgctc aacaatcggt ttgcccgcgc agccgttcgc cagtggttta tcttcaatgc
    20461 gcacgtcata gtagaactgt gtagtcgggt cgaacataca ggtgttgatg tagtccgcga
    20521 gctgctgtgc caactggcga tagcgtttgg cctcttccgg tttaccgagg atggttgcca
    20581 tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca
    20641 atagcgagta gcccagcaac gttccgtcct gactgcggtt ctcggcgaat ttcaccgtcc
    20701 agtcgctacg tttgccgccg ttagcgacat atttatccaa ctgttctttg tcgataaacc
    20761 caaagacggc ggcatcatca cgccccgatt cccacgacgc cgcaacctgt gccggaattt
    20821 ccagactgtc gtactggcct ttctccacca cgcgtgcgta gttgttcagc ccagactgcg
    20881 tctcttcttt attgcctttt ttcactgtaa acagcatctc gccattctca gtattgtggg
    20941 ctttatcgcg ggttgcgcca tattccggta cgccgttgcc gttgtgatcg cggttacgta
    21001 gccaccagtc gtgataggcc accagtttcg ggtacatctc tgccagccag tttttatcct
    21061 gggtgacgtt gtacacttcc atcaccgacc aggcggcaag gctgggtttg gtgttacgtt
    21121 cgttccagtt gccgccatcg ccgccacgct ccgggctaag gttccacgct atcaggtcgg
    21181 ggacaaagcc cacatcctgc ggacgcacgc tgtcgcctgg ctggatctgc caggagaaga
    21241 ccgcgcggat attctctttg gcgatatccg gattgaaatg cgccatcgca aacgcctgct
    21301 tccaggtatc ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggcg
    21361 tgacggtgtt atatttcacc gcaccgcccg gcgatcgcca gttaccgttg agcgtttcga
    21421 tggctttcac cgcgacgcgc gtctgctccg gcgtcgcatc cggattggtt aaccctttct
    21481 tcagatactc ttcccagcgt tgctgtgagg cagtgagata aaacgccgga cgcgccagaa
    21541 tatcgcggat ctgcatttgc tctttgctaa cttcctgagc ggtcagcaga tgggagtagg
    21601 tggtatagag cgtcgtcgaa ccgttgatat gcgccttact ggtaaagcga ttgccgttga
    21661 tttcagtctg cactggcagg gatttatgca cctgatattc tgattcaccg gaggtcagca
    21721 gatcccaggt ggcgcgcact ttgccaaaga tgactttcag gccatcacgg gtggcgctga
    21781 ttttgcgctg atagtcaggg tactcgccag caatggtttt atcggaaagc ggtttccctt
    21841 ctttcgcttc cagtttttcc agcagttcac catcccacac cagatccagc ggtttatcgc
    21901 tggtgatttt ggtttccagt agtgatgtgc gcggcgtggc gaagcgcaga atcatttcga
    21961 cctgtacatc tttcgatatc aacttttgca ccagcgcgcc gggtatactg tatgcctcca
    22021 gcgtgaaatc gactttcttg ccgtcttgcc agacggtcag gcgatcgaaa ttgctggcca
    22081 taaagttgat gtattcttcc gtcagcagcg caacgcctgg aaaaccgccc atggtgttag
    22141 ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct
    22201 ggtgatcgtc gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg ttaatcacgt
    22261 ttttataatt gtcagcgtta gcggcatggg cggaaaagct catcagcaga gcacaggcta
    22321 ctggcgttaa aatggttttg attttcatga gattactgtc cttattacca gtaaaaatat
    22381 tgcatcagga agagttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta
    22441 tcaaaagccg ttgcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa
    22501 gaggtggttt catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta agcggtggag
    22561 agataaacgc cctttaacgc ttcatacatg gcgctcacca tcatcccgct ttcacttccc
    22621 ggtacgtcca gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc aaagttaggg
    22681 gtaagcaggc ccgtgagata gagttggcct gtgccgtcgg tgatttccag accattcatc
    22741 caggtgatac cttctgtaag gtcatactgg atgtaaccgt tcatcattgc cggataggta
    22801 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    22861 tgtccggcag gtgtggcggt aacgctataa cgaaagttgc cgcttaagtt ctgtaccttc
    22921 agcgcagtca taatatcgcg ggtattggga atgacgtagc ctagatcggg cgtgaaatcg
    22981 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    23041 ccatcattgg tacgataacc gccatagaaa cggttaatcc caccttcgaa gccaccccag
    23101 ctatggtcag gcacccaggc attaccgtcg tggtcggcct gtacggtcca gccttcgccg
    23161 taaatcaggc cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtgccg
    23221 tcgctgtatt tatgtttatt ctcaccctct aaatccatat atccaccaac ccctaattcg
    23281 ccgtaaaaac gaaatgaagg agaagatgtc gaggtcattg cgggggtttt tgagtcgtca
    23341 tgttctgccg gacgtgcttc atcagcggtc acagaaaagg cgtagcaaaa cggcagcgca
    23401 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    23461 aagtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct
    23521 ccccatccag gaagagggaa aatgcctgct cattttttat tattctgctt aatcaacatt
    23581 gctggctggc gtcgcttcaa tatggcgctc ttcagccg
//
LOCUS       DAZFRG010000056        23069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFRG010000056 DAZFRG010000000
VERSION     DAZFRG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.056"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..626
                     /locus_tag="ACHKUC_001708"
     CDS             <1..626
                     /locus_tag="ACHKUC_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617976.1"
                     /translation="CSRLRSFTKARQGIIQFWAKNFAVVSKFCATALLPAHSTVRVFD
                     ANSDDRRKYYGTDLRKALKRLGKDLAVPYINEQECERADPRGDGGFDIVATIEFEDKL
                     SSNFAFLGQCGAQERDWPKKTLEAHSLKLRTYFQVHFDIPTLMFTPVFYRNSDGQWVD
                     NSPCAGVLIIDRARILQLLKKTNHCPKIVSEQWFLDFEQIINDLKVE"
     gene            complement(627..1565)
                     /locus_tag="ACHKUC_001709"
     CDS             complement(627..1565)
                     /locus_tag="ACHKUC_001709"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO6617977.1"
                     /translation="MPTVVSLFSGCGGSDAGVLRAGFNVLMANDILPYARDVYLENHP
                     ETDYILGDISGLQSFPSAELLIGCYPCQGFSQGGARKADRKINTLYLEFARALRKIKP
                     KAFIVENVSGMVRRNFEHLLKDQFKVFEEAGYTVSSQILNASHYGVSQDRKRIFIVGI
                     RKDYGITYKFPKPTHGDGLKPYSTIRDAIGHMPVWPIGEFYDADFHWYYLSRNRRQDW
                     DQISKTIVANPRHMPLHPISPTLEKLGPDKWQFTSDAPARRFSYREAAILQGFGDLIF
                     PETERASINMKYTVVGNAVPPPLFEAVAKSLPDIWD"
     gene            complement(1817..2050)
                     /locus_tag="ACHKUC_001710"
     CDS             complement(1817..2050)
                     /locus_tag="ACHKUC_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617978.1"
                     /translation="MHRIPGEIPQHKTKNIKLMAIVHRLQQIMVNENLTPDELVGCAE
                     MVRDNYRRFNDISRQKIEVFRPANMPKKLPHRM"
     gene            complement(2053..2502)
                     /locus_tag="ACHKUC_001711"
     CDS             complement(2053..2502)
                     /locus_tag="ACHKUC_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFO6617979.1"
                     /translation="MAKIHEVKLHAKYFDLVLEGKKRAEFRKNDRNYERGDTLILHEW
                     VQGVFTGRKVEARITDVTDLSDWLEDYVLLSIELLNTGAYEIVNWKELSERGLVFRIN
                     HEIMHQLGLAVMYESVTGLSGGAVVAADGMWSYSDEQVECAKQKGWF"
     gene            complement(2502..4787)
                     /locus_tag="ACHKUC_001712"
     CDS             complement(2502..4787)
                     /locus_tag="ACHKUC_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6617980.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFATRISSQLFIMYEELCDAWVDA
                     HGEKESLFTDEAQDHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTTPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGRRTPNRQ
                     QRNGSPLKPYEMAPSARLTRSERMQITRIRVDLAQKGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMEWK"
     gene            complement(4777..5052)
                     /locus_tag="ACHKUC_001713"
     CDS             complement(4777..5052)
                     /locus_tag="ACHKUC_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO6617981.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            complement(5049..5273)
                     /locus_tag="ACHKUC_001714"
     CDS             complement(5049..5273)
                     /locus_tag="ACHKUC_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6617982.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCCASALVCEECDTPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(5276..5575)
                     /locus_tag="ACHKUC_001715"
     CDS             complement(5276..5575)
                     /locus_tag="ACHKUC_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO6617983.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(5575..5799)
                     /locus_tag="ACHKUC_001716"
     CDS             complement(5575..5799)
                     /locus_tag="ACHKUC_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO6617984.1"
                     /translation="MHTVSENQCGIYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQVSEKFHNIGLSRGEVL"
     gene            complement(5863..6363)
                     /locus_tag="ACHKUC_001717"
     CDS             complement(5863..6363)
                     /locus_tag="ACHKUC_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO6617985.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(6360..6530)
                     /locus_tag="ACHKUC_001718"
     CDS             complement(6360..6530)
                     /locus_tag="ACHKUC_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6617986.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(6533..6805)
                     /locus_tag="ACHKUC_001719"
     CDS             complement(6533..6805)
                     /locus_tag="ACHKUC_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO6617987.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKLKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            6942..7235
                     /locus_tag="ACHKUC_001720"
     CDS             6942..7235
                     /locus_tag="ACHKUC_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6617988.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            7305..8285
                     /locus_tag="ACHKUC_001721"
     CDS             7305..8285
                     /locus_tag="ACHKUC_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6617989.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSIRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(8472..8972)
                     /gene="cpxP"
                     /locus_tag="ACHKUC_001722"
     CDS             complement(8472..8972)
                     /gene="cpxP"
                     /locus_tag="ACHKUC_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO6617990.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            9122..9820
                     /gene="cpxR"
                     /locus_tag="ACHKUC_001723"
     CDS             9122..9820
                     /gene="cpxR"
                     /locus_tag="ACHKUC_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO6617991.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            9817..11190
                     /gene="cpxA"
                     /locus_tag="ACHKUC_001724"
     CDS             9817..11190
                     /gene="cpxA"
                     /locus_tag="ACHKUC_001724"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO6617992.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(11362..12036)
                     /gene="yiiM"
                     /locus_tag="ACHKUC_001725"
     CDS             complement(11362..12036)
                     /gene="yiiM"
                     /locus_tag="ACHKUC_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO6617993.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGVVSADAPL
                     ELVSRVSDMTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(12185..13167)
                     /gene="kdgT"
                     /locus_tag="ACHKUC_001726"
                     /pseudo
     CDS             complement(12185..13167)
                     /gene="kdgT"
                     /locus_tag="ACHKUC_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(13427..14047)
                     /gene="sodA"
                     /locus_tag="ACHKUC_001727"
     CDS             complement(13427..14047)
                     /gene="sodA"
                     /locus_tag="ACHKUC_001727"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO6617994.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            14333..15367
                     /gene="rhaT"
                     /locus_tag="ACHKUC_001728"
     CDS             14333..15367
                     /gene="rhaT"
                     /locus_tag="ACHKUC_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO6617995.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(15364..16212)
                     /gene="rhaR"
                     /locus_tag="ACHKUC_001729"
     CDS             complement(15364..16212)
                     /gene="rhaR"
                     /locus_tag="ACHKUC_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO6617996.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(16286..17122)
                     /gene="rhaS"
                     /locus_tag="ACHKUC_001730"
     CDS             complement(16286..17122)
                     /gene="rhaS"
                     /locus_tag="ACHKUC_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO6617997.1"
                     /translation="MTVLYSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPLR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            17410..18879
                     /gene="rhaB"
                     /locus_tag="ACHKUC_001731"
     CDS             17410..18879
                     /gene="rhaB"
                     /locus_tag="ACHKUC_001731"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO6617998.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            18876..20135
                     /gene="rhaA"
                     /locus_tag="ACHKUC_001732"
     CDS             18876..20135
                     /gene="rhaA"
                     /locus_tag="ACHKUC_001732"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO6617999.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
     gene            20228..21052
                     /gene="rhaD"
                     /locus_tag="ACHKUC_001733"
     CDS             20228..21052
                     /gene="rhaD"
                     /locus_tag="ACHKUC_001733"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO6618000.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIIHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            21062..21376
                     /gene="rhaM"
                     /locus_tag="ACHKUC_001734"
     CDS             21062..21376
                     /gene="rhaM"
                     /locus_tag="ACHKUC_001734"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO6618001.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(21417..22811)
                     /locus_tag="ACHKUC_001735"
     CDS             complement(21417..22811)
                     /locus_tag="ACHKUC_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6618002.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT         5921 a         5854 c         5513 g         5781 t
ORIGIN      
        1 catgctcgag gttacgttct ttcacaaagg ctaggcaagg aataatacag ttttgggcaa
       61 aaaattttgc tgtcgtaagt aaattttgtg caacagcact actcccagca cactcaactg
      121 tacgagtatt tgacgcaaac tcagatgaca ggcgaaaata ttatggtact gatttaagaa
      181 aagccttgaa aaggctagga aaagacttag ctgttcctta tattaatgag caagagtgcg
      241 aaagagctga tccgagaggt gatggtggtt ttgatatagt tgcaactata gaatttgaag
      301 ataaattaag tagtaacttt gcctttttag gtcaatgtgg agcccaagaa agagattggc
      361 ccaaaaaaac acttgaagcc cactcattga aactacgaac atattttcaa gtgcattttg
      421 acataccaac tctaatgttt acccccgtat tctatcgaaa ctcagatggt caatgggtag
      481 acaatagccc ttgcgctgga gtattgatca ttgacagagc aaggatatta cagcttttaa
      541 aaaagacaaa ccattgccct aaaatagttt cagaacaatg gtttcttgat ttcgaacaaa
      601 ttatcaatga tcttaaggtc gaataactaa tcccaaatat ctggaagaga tttagcaacc
      661 gcctcaaata aaggaggcgg aacagcattg ccaactactg tatacttcat attaatagaa
      721 gcccgttcag tttctggaaa aattaggtct ccaaaaccct gtaaaatagc ggcctctcga
      781 taactaaacc tacgagctgg tgcatccgaa gtaaattgcc acttatctgg acccaatttt
      841 tctaatgttg gacttattgg atgtagaggc atatgtctag gatttgcaac aattgtttta
      901 gatatctgat cccaatcttg ccgacggttg cgcgatagat aataccaatg aaaatcggca
      961 tcataaaact caccaatagg ccaaacaggc atatgaccaa tagcatcacg aattgttgaa
     1021 tatggtttca agccatcacc atgtgttggt tttggaaatt tgtatgtaat accatagtct
     1081 tttcgtattc ctacgataaa gattctcttc ctatcttgtg atacgccata gtgggacgca
     1141 ttcagaattt gcgagcttac tgtataacct gcttcttcga aaactttgaa ttgatccttt
     1201 aacaaatgtt caaagttacg ccttaccatt ccagaaacat tctctacaat gaatgctttt
     1261 ggcttaatct ttctcaaagc acgagcaaac tctaaatata gagtattaat ctttctatct
     1321 gccttccttg ctccaccctg gctaaatccc tggcaaggat agcatccgat aagcaattcg
     1381 gcagaaggga acgattggag tcctgaaata tcgcccaata tgtaatcagt ttcaggatgg
     1441 ttttctaagt aaacgtccct tgcataaggc aaaatatcgt ttgccatcag cacattgaac
     1501 cctgctctca aaactcccgc atcagaacca ccacatccag aaaaaagtga aactacagtt
     1561 ggcatcgccc ctcctaaaaa ccgaccgcgt attatagcga aaccacccct tgggaaaagc
     1621 tagatttcgc caagttttga tattctcatg ttttagtagt tgtggccatc tttaacgaga
     1681 gaaagatgaa attgagttct cattaatttt caataggttt aattgtaaac acagcaggaa
     1741 tcaaagctaa cacgtcgcta cgctcattgt tcaaccccgc catctctgaa aacaagtttc
     1801 agaaatggcg agtatactac atcctatgcg gtagtttctt aggcatgttt gctggtcgaa
     1861 atacctcaat tttctgtcta ctgatatcgt taaaccgtct gtaattatcc cggaccattt
     1921 cggcacaccc gaccagctca tcaggcgtca gattttcgtt gaccataatc tgctgtagac
     1981 ggtgaacaat ggccatcagc ttgatatttt tagttttatg ctgaggtatc tcgcctggta
     2041 ttctgtgcat tttcaaaacc accctttttg ctttgcacac tccacctgtt catctgaata
     2101 gctccacatc ccatccgcag caaccactgc tccgccagac aggcctgtca ctgactcata
     2161 cataacagca agaccgagct gatgcataat ttcatgatta attctgaata ccagaccacg
     2221 ctcactaagt tctttccagt tcacaatctc atatgcgcct gtattaagca gctcaatact
     2281 tagcaagaca taatcttcca gccagtctga caggtcagta acatctgtta tccgggcttc
     2341 aacctttcgc cccgtaaaca caccctgtac ccattcatgc aaaatcagcg tgtccccgcg
     2401 ctcataatta cggtcatttt tccgaaactc tgcacgtttc tttccttcca gcacaaggtc
     2461 gaaatatttt gcgtgcagct ttacctcgtg aatttttgcc atcatttcca ctccattact
     2521 gttgagaatc ccggccactc atcagcaacc ggatacgtga attttttccc gtcataattt
     2581 acggttgctc cacgcgccag cgcctcaagt tcccatcgct gcggcctgat acccttttga
     2641 gcaaggtcaa cacggatacg ggtaatttgc attcgttccg accgggtcag tctggccgat
     2701 ggtgccattt catatggttt taacgggctt ccgtttcttt gctgacggtt tggcgttctc
     2761 cgaccgtgtt ttaatgcgcc cctgagcgcc ctcacgacct ccgggtcatt ccattcgata
     2821 acaccgtcat caaccagatt aagcactgct gcggcgtgct cagaaggtgt gggagccggt
     2881 aacgaagtat caccaccggt gagctttcca cagttattga caggactccg aggcgcggcg
     2941 atgccgcttt ttaaagtcaa aggctcaacg accggaactt tcggcacaat gcgccagtcc
     3001 gtcgttctgg tgatatgaat atgacgcgcg ccgagatgcg gcgcgtaaat gccgaccact
     3061 ctctcgactt cttcctcgta ctcgttaacg tcatccgacg ggctacgggc gactctgaca
     3121 gtctggcaat cacgcggaac atttgcccca ccctgcgcgc tgatatacaa cgcaaaatca
     3181 ccactgtctg cggcggcacg tgcagcctca acgcgttcgt caaactcatc agcaatgctg
     3241 acgccacgag gcaatttgcg tagttcacgg taagccccca ttgtcggcag gccaaccgtt
     3301 ttaaattgcg ggatgcgcca cgttgacgcc catgcggtaa cagccgcagc agtatctttc
     3361 agcggcctgc cggtatcgtt atcgagctga ccatccagtg catagccgtc gatgtttttt
     3421 gagatgtatt tcgcgatata ccccgcagca ccgccccggt taagatgttt tgcctgaaaa
     3481 cggtttcgcg cggctcctct ttcgtctcca tcctctttga gcgcgtagcg acgcatgatt
     3541 tcaataatct ggttacgctg gcgtggatta caaaaaagca tcatatgcca gtgcggcgtt
     3601 ccgtcgtggt gtggctcgac gacacgcaaa ccgtagacct gtaaatcatt atccttgaat
     3661 gccgtgcgca tcaggctcca gatacggcag agataacgct gcgcatcctt cggattaaat
     3721 gcctcatcgt tccagccgtg attaagctgg acggttttac tttctccttt tccgacctga
     3781 cgtgtcgggt gatactttga cggcgtggtc agcgtgataa acatccccac atcaccctct
     3841 gcggcggcgt aacgctcaat accggcaatg gtgttcatca gctccatccg gcgaatttca
     3901 ggattagaaa tactgcccat caccttactg ataaggtcga tgcgctcgcc ggtttccctg
     3961 ttttcaaggt cacatgattt aagaaactcc agatttgcct ggcggcgcgc acgcacatca
     4021 cgaatggcat gtttactggc ataaggagaa cggtctttat tgacctcccc gacagcaatc
     4081 agtaacgcct catgccagcg catacgctgg cctttaagct gatgagtcca ccactcatcg
     4141 ttaaacagac gggcaatggc agaataagcc tgcctcgtgg tcatctgtcc tttacggtat
     4201 tttttccagt agagagggga aatattgaaa gctcgtgcag cgccagcaac atgaccatac
     4261 aggtgatcct gagcctcatc cgtaaacagt gattctttct cgccatgcgc atccacccag
     4321 gcatcgcaga gttcctcata cattatgaaa agctgcgatg agatacgggt ggcaaacttt
     4381 ttcagttcct tgtcattcat tccaggcagg cgcgcatagt ggtcacgctc tgccagaaac
     4441 agcaacgacg cgtcggtgtt catttcatgg cgctgattaa cgcgctcaat gcgcggccat
     4501 aaacgacgct gaaaagtgga tgtgaggaaa taaaacccgt gcaccgggct tttattgcgc
     4561 cggatgtagt catagcgtga agtaaacagc gagcgcaaaa agtaaggcag gcggttaatc
     4621 gtggataaaa caccttgcac ctgacgcatc tcgtcacgtg taaggggtct ttcgcgcccg
     4681 acggcctcgc gtggcgcgtt ccatgcataa gcaccggtaa acgtcttacc ggtgcctgcg
     4741 gcaaatgctg acggagggac aaaacgcccg gaggctttaa cggccatatg agccaaaagc
     4801 ctctgaacaa cgcctgctga gttgctcaac ctgcgcgttt aaatcagcaa aagactttgc
     4861 gcttccggtc agaatatcgt gatgcatcag gccggaaacg agctggctta atttcgggta
     4921 ataaccaacc accgccagcc attcctgacc ggcgttttta ccgctttcag ctctcttttt
     4981 ctcgtggaga ataaactgaa agctgtcact ggtaacgaca taacgttcgc caatttcaat
     5041 acgaatactc atgccattct ccggtaatgc ttgttttttg cttcaaagac tgactggcag
     5101 gaaacacaac gcgtggctga cggataagcc gcacgacggg cagcaggtat tggcgtatca
     5161 cactcttcgc aaaccagcgc agaagcacag caatgtttta cccttgccgc gttaatctgg
     5221 cgctccagta attcagcctg ttgttcctga ataaaatcta cgttgtccgg cattatcagc
     5281 tccttttatc gttaagtttc ctggatacat cagcgcaata actggcgagt tctgtcgtta
     5341 attttgtcag ttcatccact gaggaaattt gcttgtggaa cacagcgcgt ttaacaagta
     5401 aattgaccac atcagacagg aggtttaatt cattctgata aatcgcgata acagattcag
     5461 ttatgtcgcg tttttcttta tcaagacaaa gttgaataag agacaaatca ccattttcca
     5521 taacggcgat ttttaaggcg ttattcagta atacaactga atgagaacag gacatcaaag
     5581 tacctccccg cgagacaatc cgatattgtg aaatttttcc gacacctgac tgagcagctc
     5641 gactatctcc acgcgggata actccgcctt tgtgatgtgg cgaatcatgg cgtcaagatg
     5701 agaagaaaag cgcgtcgcag cgtcggcctg tgcttcggtt ctggcctgtt gcagcagtaa
     5761 tgcgtatata ccgcactgat tttcagaaac tgtatgcatg actttctcca ggcaaaaaga
     5821 agccccgcac gattaagtgc gttaaaaact ctggttaatt atttaatgca gatattgctc
     5881 tggttttacc gacgtcagaa ttgtcggtgc atactcaaac aggctgaata attcacgtaa
     5941 tgcacggaat aaagcatcac gccagtaaca tgattcttca ttaattcgcc agtatggctg
     6001 gttgaattct ttttcagtca atccggcatg cataaataaa gtacgacgct gactgactgt
     6061 taaaaaacta atatatgcat actcacttgc gccaacctga cggcgttttg agaatgcccc
     6121 acgcaattca tcaattgcac aaaccagccg ttcacgttcg acgtcgttca tttcttcaaa
     6181 acgcatcgtt gcgtgacgct gttttaactg cgcatgaaag caaaccgtta accgttcgcg
     6241 ctccatcatc tgattataat aatcacatgt atcctgccag cgagggacgg caagatgctt
     6301 accaattatc cggcgcatag ctgctggctg tttttcaacg agattgagcg tcatcactgt
     6361 catttccata ccctccggct tttcagaaag gtcagagcct tctttaacgg actctgtttt
     6421 ttggtgcgga taatgattcc cttgcgcccc ttcccgtggg tgatggtgaa gtcaatcgcc
     6481 ctggggcttt cgttacgcag taactgagca atacaacgcg gctcattcat aatcacaacc
     6541 ccatccacaa aagccatgca tcacgctgtt caaccggtcg gttataaaac gcctcacgta
     6601 cagcgcgatt aaactcagga atgaaaaccc atttttcacc ggcacgagcc ttcagtttgc
     6661 aaggatcacg caattcaata attggtaatt tatttgcctt caccatttca ctgacggctg
     6721 tctttggctt ccctaataaa tcagcaaatt tatccacatg aaccgcatca agcggatact
     6781 gaatcacata attttcagcg tccatatatg gtaccctcat aggatccagc cctttctaaa
     6841 ccactcaaaa ccgtttagac gctggtttat tctcaaatca atggaaccta tataggttcc
     6901 agttttgagg gaatttagtc cctatatagg caccatgtca aatgaaatta agcgaaaaga
     6961 ttaaggcctt gcgtgaggct gaagggctaa gccaatcaaa attctgtgaa atcatagagt
     7021 taccgctaag cacacttaaa aaatatgaag gaggaaactt tgaacccggt ggcacagctt
     7081 tgctaaaaat cactatgcat cccacattcc aaaaatatgc tctatggctt atgacagata
     7141 aaaccgcgcc ggacgcagga caaatcgcac cggctctcgc gcacattggg ccagagtcaa
     7201 cagagtccaa ccactccgcg aaaaggattg gctaactcta tataaagatt acattttcac
     7261 catttgctac caagatggtg aatacagcgc cggagggctt tcttatggca attaagaagc
     7321 tcgatgatgg tcgctatgaa gtggacatta gacctcgcgg tcgcgacgga aaacgcatcc
     7381 gcaggaaatt cgaaagaaaa gctgaagcac tagcatttga gcgatacaca atcgccaatg
     7441 ccagtcagaa agaatgggga ggccagcgag cagaccgccg aactttgagt gagttgctgg
     7501 acatctggtg gaaatatcac gggcaaaacc acgagcatgg aacaaaagag tttaatcatc
     7561 tactcaaaac catcagcggc ataggtgata taccagtgag ccggatgagc aaaagggctt
     7621 tgatggatta tcgttccata cgactacgtg atggtatcag tgccgcaacg ataaaccgtg
     7681 acatgtaccg attatccggc atgttcacaa aattaattca attggatgaa ttttccgggc
     7741 aacacccaat tcacggactg ccgccactgg cggaggccaa ccctgaaatg acgttcctgg
     7801 aaaaagcaga aatcgaaaaa ctgttaaatg ttttggctgg tgatgactta cttgtcgcgc
     7861 ttttatgtct gagcactgga ggaagatgga cggaagttgc cacgctaaaa ccagcacaga
     7921 ttacaaattg cagggttacc ttcctgaaaa ccaaaaacgg taaaaagcga accgtgccga
     7981 tttctgagga actggagaaa aaagttaaag aggaggcaag cgccaaatta ttcaaagttg
     8041 attatgagaa attttgcggg attttacgca gagtgaaacc tgatatacca cccaatcagg
     8101 caacccacat cctgcggcat acattcgcaa gccatttcat gatgaacggg ggcaatataa
     8161 tcgcactgca acagattctg ggacatgcga gcattcagca gacgatggcc tatgcgcacc
     8221 ttgcgcctga ctacctgcaa aatgccgtcg ctctgaatcc actaaaaggc ggagtgacgt
     8281 tataaatttc cccctctgag tgtccacata gtgtccacac tcaagaaatt ttgaaaccac
     8341 tccaaaccct tacagctttc gataagtgac tgtttttact tttgattgca tgtaagtaat
     8401 tgataaaaaa aacccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa
     8461 aacagggttt actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt
     8521 ttttgccatt gcgtcacgtc gcgcaactgc tccattcgtt gttgatgttt ctcgtttaaa
     8581 accgcttgct gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca
     8641 acctgacgag ctatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca
     8701 tcaaaatttt ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga
     8761 ggctgttcgt gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt
     8821 aaacttatgc cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga
     8881 tgccagttat cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt
     8941 gaggccatga cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg
     9001 attcaacgat agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa
     9061 cgtaaagtca tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac
     9121 aatgaataaa atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct
     9181 gctcgagatg gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct
     9241 tctggacgac agcattgatt tacttttgct tgatgtaatg atgccgaaga aaaatggtat
     9301 cgacacatta aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg
     9361 cggcagtgaa cttgatcgcg ttctcgggct tgagctgggc gcagatgact atctcccaaa
     9421 accgtttaat gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg
     9481 gagcgaacaa cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttaag
     9541 tctcaatcct ggtcgccagg aagccagctt cgacgggcaa acactggagt taaccggtac
     9601 tgagtttacc ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgagca
     9661 tttaagccag gaagtgctgg gcaagcgcct gacgcctttc gaccgcgcta tcgatatgca
     9721 catttccaac ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac
     9781 cctgcgtggt cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc
     9841 atcttcgcca tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc
     9901 aagctcgatt cacgccagat gaccgagctt ctggatagcg aacagcgtca gggtctgatg
     9961 attgagcagc atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg
    10021 cggcgtctgt tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg
    10081 accaccgaag gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt
    10141 attggtcagg ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg
    10201 gtcggtccgt tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg
    10261 gccagcagtt ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg
    10321 attgtcacca tgctggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa
    10381 ccggcgcgta agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac
    10441 ccggaactgg aagcggggcc acaggaattc cttgccgccg gtgccagttt taaccagatg
    10501 gtcaccgcgc tggagcgcat gatgacctct cagcagcgtc tactttctga tatctctcac
    10561 gaactgcgca ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc
    10621 ggtgaaagca aggaactgga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc
    10681 aacgatctgt tggtgatgtc acgtaatcag cagaaaaatg cgctggttag cgagaccatc
    10741 aaagccaacc agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg
    10801 ggcaagtcgt tgacggttaa cttcccacct gggccgtggc cgctgtacgg caacccgaac
    10861 gccctggaaa gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag
    10921 attgaagtgg gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt
    10981 cctggcgtta gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa
    11041 gcacgcgatc gtgaatctgg cggtacaggt ttggggctgg cgattgtaga aaccgccatt
    11101 cagcagcatc gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg
    11161 atttggttgc cgctgtataa gcggtcataa cgtcaacatt caggtaaaaa aaatgcctga
    11221 tgcactacgc ttatcaggcc tacaaaacct gttgaattta tgggttttgt aggcaggata
    11281 aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct
    11341 tatcaggcct acactccggg gttattttcc ccacaaacgg cgggaaaaat cttcgatctt
    11401 gccgctaagt cggcgctttt gcatcgttcg cgtccagctt ttcgataacc ctgcggcgga
    11461 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    11521 ctgcacggtc atatcgctga cacgggaaac caactccagc ggcgcatcag cagataccac
    11581 ccctggtgca attacgctat acaaccaccc cactttaccc gtgttttgca tcaactgcgc
    11641 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggtgagcgtg gctggctgac
    11701 ctgaattaat gcctctcccc agcggaaaat atcaccaata taaacattac tttccgtcag
    11761 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacagctccg cctgttccgg
    11821 aaattcccgc gcccagtaga gataatgctc acgaggataa tgacacagcg ctctgtctgg
    11881 cccgccgtgg atctttttct ccgcctgttc gtcaccttcc agccccaggt ccgtcagcat
    11941 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    12001 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    12061 tcccattaga ccatatttat tttatgtcag gcgtgctgtt ttcgcggtaa gtatccaggc
    12121 agaaacgcaa ttgaaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    12181 aggtttattt taccgtgcct aaaatttcga ttttcgccac tctggctttg actttacgtg
    12241 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgcgacca
    12301 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttcagca atcagcacag
    12361 gtgtcgctac cgcggcccct gcggaactgg aagcagcaat tccggcagtg ccatcgccac
    12421 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt actgccacac
    12481 ccaacaggat ccccagcaaa cctgtctggg caattacagt caaatcaatg gtattgccca
    12541 gtgcgaaggc aaagaatgga atcagcgtct gcaccgcttt gctgaaaaat tctcgtaact
    12601 cagggtcaag gttccccagg gcaaagccca ccaggaacgg caatactgcg ccgacgaaaa
    12661 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    12721 cggactccaa cgacatcaac acaaatgccc cagcttcttc ttttgtgccg tactgctgca
    12781 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccaacgttg
    12841 aaagtccggc aaaaaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    12901 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    12961 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    13021 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    13081 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc cccccgggat
    13141 tttctcaatc gagcgtttta tctgcatttc catcacctgt cggcatattt ttaagggctg
    13201 atgcgtcatt cctcaacgga gcatcagtgt tgatggaaac gaagatagac attcatttga
    13261 tcaataaaag tgatcaaaac cacatcaatt gaaacgctgt tttattttta tcggatcatt
    13321 gtttttattt tgaaattgat cacaaaaaaa ccaccgccgt tggcgatggt tcatttttta
    13381 agctgcaatg cggcgtaaac gcctcattgc agcaggcgtc aaatgattat tttttcgccg
    13441 cgaaacgtgc cgctgcttcg tcccagttca ccacgttcca gaactcttta atgtagtccg
    13501 gacggcggtt ctggaatttc agatagtaag catgttccca cacatccagg cccagaatcg
    13561 ggaagccgga agcgccagaa atggcttcgc ccatcagtgg agagtcctgg ttagcagtag
    13621 aaaccaccgc cagtttatcg cctttttgca ccagccatgc ccagccggaa ccaaagcggg
    13681 aagctgccgc tttttcaaat tctgctttga aattatcaac ggaaccgaaa tcacgttcga
    13741 tagccgcttt caggtcgccc tgcagggtgg tgcctttttt cagacctttc cagaacagac
    13801 tgtggttagc gtgaccacca gcgttattgc gcagtacggt tttcttgtct gctggcagct
    13861 ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc aaattctggc aggttttcca
    13921 gcgccgcgtt ggcgttgttg acgtaagtct gatggtgttt ggtgtggtgg atttccatgg
    13981 tctgcttatc gaagtgcggt tccagggcat cgtaagcata cggcagggat ggcagggtat
    14041 aactcatatt catctccagt gttgtcgggc ggccgattgt taatgccgcg taagcagttg
    14101 gttcattata gttaattaaa tgatattgaa aatgattatc aatgccgtac ttttcataag
    14161 ggtatggttt tgcaggaaaa tgcccaagat gtgaagcaaa tcacccactt aatgccgtga
    14221 ttgccagtaa atcggcagcg gcggcaacag gcgaaaggtt aatcgacagc tcaattttta
    14281 cactcatctc gtcggagatg tgacgcgacg aaaaaatgat gaggataaga agatgagtaa
    14341 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    14401 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag ttggtgggat
    14461 tgtttcgtgg attatcctgc cgtgggccat cagcgccctg ttactgccga atttctgggc
    14521 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    14581 ggggatcggt aatatcaact acggcctgac catgcgttat ctcggcatgt cgatggggat
    14641 tggtatcgcc attggtatca cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    14701 caatttcgat gttctgatta ataccgaagg cggacgcatg acgttgctcg gcgttctggt
    14761 ggcgctgatt ggcgtaggga ttgtgactcg cgccgggcag ttgaaagagc gcaagatggg
    14821 cattaaagcc gaagagttca acctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    14881 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    14941 cgcactgggc gtcgatcccc tgtatgtcgc tctgccaagc tatgttgtca tcatgggagg
    15001 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    15061 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaacgtgt tactctcagc
    15121 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctggggcc acgcccgcat
    15181 tccggcgcag tatgactaca tcagttggat gctgcatatg agcttctatg tattgtgcgg
    15241 cggtatcgtc gggctggtgc tgaaagagtg gaacaatgca ggacgccgtc cggtaacggt
    15301 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    15361 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    15421 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    15481 gattaacagg cggctatgct ggaggagata ttgcgcatga cacactctga cctgtcgcag
    15541 atattgattg atggtcattc cagtctgctg acgaaattgc tgacgcaaaa cgcgctcact
    15601 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    15661 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    15721 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    15781 agcaaacgac acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    15841 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    15901 aatcgctccc tgccagtcaa ggttcagctt cagccgctcc gggcaataaa taatattctg
    15961 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    16021 atcgccacgg gtaatgcgat aagggcgatc gttgagtaca tgcagaccat taccgcgcca
    16081 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    16141 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    16201 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    16261 tacaaatacg ttgagaagat tcgcgttatt gcagaaaacc atcccgtccc tgacgaatat
    16321 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    16381 atccgcagcg ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg
    16441 ctttcatcag tcgcaggcgg ttcaggtaac gcagaggcgt cagccccgtt tgctgcttaa
    16501 gctggcgatg cagcgtacgc aatgaaagag aaaattgatc cgccacagcg tcccaattca
    16561 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    16621 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacagaa
    16681 tttcacgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    16741 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    16801 catcctgctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    16861 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    16921 gatcgcgtac gaaacagacc gtcccaccgg tgatggtata gggctgccca ttaaatacat
    16981 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    17041 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat
    17101 ccacactata taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    17161 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    17221 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaatgaag
    17281 caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    17341 agtaacgaga aggtcacgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    17401 gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc
    17461 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    17521 tttaacaatg gcctgcatag ccagaacggt tatgtcacct ggaatgtgga tagcctggaa
    17581 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    17641 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    17701 ctgcccgttg cttatcgcga tagccgcacc aatggcctaa tggcgcaggc acaacaacaa
    17761 ctcggcaaac gcgatattta tcaacgtagc ggcatccagt ttctgccctt caatacgatc
    17821 tatcagctac gtgcactgac agagcagcaa ccagaactta ttccacacat tgctcacgct
    17881 ttgctgatac cggattactt tagttatcgc ctgaccggca agatgaactg ggaatatacc
    17941 aacgccacga ccacgcaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    18001 gcgtggagcg gtgccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcata
    18061 ggtcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    18121 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct
    18181 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    18241 gcagccaata tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    18301 atgggcttat ggctgcttca gcgagtgctt caggagcggc aaatcaacga tcttccggcg
    18361 cttatcgccg caacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    18421 cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg
    18481 caaccgatcc cggaaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg
    18541 ctgtatgccg atgtgttgca tgagctggcg cagctgcgtg gtgaagattt ctcgcaactg
    18601 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctgtgtgc cgatgcctgc
    18661 ggtattcggg tgatcgcagg gcctgttgaa gcctcgacgc tcggcaatat cggcatccag
    18721 ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcactacc
    18781 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    18841 attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    18901 gggagcttgc gaaacagcgt ttcgcggcag tagggattga tgtcgaggag gcactgcgcc
    18961 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    19021 aaaacccgga aggtttgctg accggtggta ttcaggctac tggtaattat ccgggcaaag
    19081 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccagggc
    19141 cgaaacggct taatttacat gccatctatc tggaatcaga cacgccagtc tcgcgcgacc
    19201 agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    19261 tggattttaa cccctcctgt ttttcacatc cgctaagcgc cgatggcttt acgctttccc
    19321 atgccgacga ccgcattcgc cagttctgga ttgaccactg taaagccagc cgccgtgttt
    19381 cagcctattt tggtgagcaa ctcggcacac cgtcggtgat gaacatctgg atcccggatg
    19441 gcatgaaaga tatcaccgtt gaccgcctcg ccccgcgtca gcgtttgctg gcagcactgg
    19501 atgaagtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    19561 tgtttggcat tggcgcggag agctacacgg ttggctccaa tgagttttac ctggggtatg
    19621 ccaccagccg ccagacggcg ctgtgcctgg acgccgggca tttccacccg actgaagtga
    19681 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    19741 gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcgattg
    19801 ccagtgagat tgttcgtcac gatctgttcg accgggtgca tatcggcctc gacttcttcg
    19861 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    19921 tgctgcgtgc gttgctggaa cctaccgctg aactacgcaa gctggaagcg gcgggcgatt
    19981 acactgcgcg tctggcgcta ctggaagagc agaaatcgtt gccgtggcag gcggtatggg
    20041 aaatgtattg tcagcgtcac gatacgccag cgggtagcga atggctggag aacgtgcgga
    20101 cttatgagaa agaaattttg agtcgccgcg ggtaaacact gccggatgcg gcacaagtgc
    20161 cttatccggc ctacagatcg tacatccggc aacaggcacg gacgtccaat taacaggaac
    20221 acagaacatg caaaacataa cccagtcctg gtttgtccag ggaatgatca aagccaccac
    20281 cgatgcctgg ctgaaaggct gggacgagcg caacggcggc aacctgacgc tgcgcctgga
    20341 tgacgccgat atcgcaccat atcacgacaa ttttcaccaa caaccgcgct atatcccgct
    20401 cagccagccc atgcctttac tggcaaatac accgtttatt gtcaccggct ccggcaaatt
    20461 cttccgtaac gtccagcttg atcctgcggc taacttaggc atcgtaaaag tcgacagcga
    20521 cggcgcgggc taccacattc tttgggggtt aaccaacgaa gccgtcccca cttccgaact
    20581 tccggctcac tttctctccc actgcgagcg cattaaagcc accaacggca aagatcgggt
    20641 gatcatacac tgccacgcca ccaacctgat cgctctcacc tacgtgctgg aaaacgacac
    20701 cgcggtcttc actcgccaac tgtgggaagg cagcaccgag tgtctggtgg tattcccgga
    20761 tggcgttggc attctgccgt ggatggtacc cggcacggac gaaatcggcc aggcaactgc
    20821 acaagagatg caaaaacatt cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg
    20881 accgacactg gatgaaacct tcggtttaat cgacaccgca gaaaaatcag cacaaatatt
    20941 agtgaaggtt tattcgatgg gcggcatgaa acagaccatc agccgtgaag agttgatagc
    21001 gctcggccag cgtttcggcg ttacaccact cgccagtgcg ctggcgctgt aaggagcaaa
    21061 catgatccgc aaagcctttg tcatgcaggt taaccccgac gcccacgaag agtatcagcg
    21121 tcggcataat ccaatctggc cggaactgga agcagtgctg aaatctcacg gtgcgcataa
    21181 ctacgccatc tatctcgaca aagcgcataa tctgctattt gccacggtag agattgaatc
    21241 tgaagaacgg tggaatgcgg ttgccagtac agatgtttgc caacgttggt ggaaatatat
    21301 gaccgacgtt atgcccgcta atgcggataa cagcccagtc agtagcgaac tgcaagaagt
    21361 gttttacctg ccttaatgag ttgcggtgcc ggaaaatatc cagcaccgcc ctttcatcag
    21421 aagaagtatt taaagcgaac gcgtgcgccg taacggtcat cactatcgtc gccgtgtatc
    21481 ttatcgccgt cgctatccag aatggagtaa tacgtgccga ggtaaatatt aaagttctcc
    21541 atatccatca cattagcgaa ctgataagac gcatgaatgg tgtgaatgtt gtatgttcct
    21601 tcatcatcaa tccagcaatc gttatcacaa accacgccac taaattcatc aatcttatta
    21661 tgtgcataga tataacccag ctcgaaacgt ttccacagcg cgttaatccc tgccgtgaaa
    21721 tctttttcat tattagcatc taaataggcg gtattaagat taaccacgat gccattttcc
    21781 ggatcggttt tcaggccatt ccaggtcata ctcatgccat aaccggtacg atcggactga
    21841 tcgacaaaat tacccttgct atcggtataa ccataagcat tatttaccac attcgcttcc
    21901 atcgctgcgg aaacggtgaa ttcttccgtc ggcgaccagg caattaccgg acgcagataa
    21961 gcaacatttt tctgctgttc catatcgcgt ccatgataat taccgtcgtt atataaagat
    22021 gttccgtctt ccagtaacgt gtttaattca aaataccagt tatcgagttg tttgctgacg
    22081 aggaaattac cgccagcgtt agaacgtccg cggccctctt tcatcatata gatataaccg
    22141 ctgccatcgt cataaagatc gttcgcagta ttaccggaat gttcaacaaa ggtatcctga
    22201 ttcagcggga acatatcgta ggcttcaaaa cggcctactt tgaccttcca gtcgttttct
    22261 ttgccaaaga agaaaaccgc atcatcgata tttactgaac cgtgcatatc ccccagcggt
    22321 tgtgcggaga acccagcgaa atagccatta tccagttttc gcataccatc aaaacctaac
    22381 agaatacgac cattgagatt ccactgttca ttagtgggat cgctattcac atcagcgttg
    22441 gtcattgcca gcaggccatg attactttcc gcatccatat taaattctac gtcaccgtag
    22501 atttttaaat cgccataccc tgaaagcagg agattaggcg gggtggcgtt tttcttctct
    22561 gactccgtcg ttgattgacc gtttaccggc gtgttgccct gtgcggtttt aatttcgcgg
    22621 atctcggcgg cttgttgtgt ctgaagctgt ttaacctgcc cctctgcttt tgctgcccgc
    22681 atttctgccg cttccaggcg ttcttccagc aatgccatgc gttgttccag cgttaaatgc
    22741 tgtgtttttg ccataccggt actacatgcc aataataaac cgatggctaa agataatgtc
    22801 gattttttca ttatttacgt tccctgaaat aaaggagacc ctacgctttc caaaaggaat
    22861 acgtatatag agaaatataa ggtgtgcact ttcgaatttt taatccaact tcagcaggcc
    22921 gatggatagt atcccgccgc ataatatgag taaatccgct gatttcctgg aacgtgagga
    22981 atataacaat agtgccgttt ttaactaaat atcgtaaaaa atattagata tgattcacgc
    23041 tgactcataa gccacaaata taaataacg
//
LOCUS       DAZFRG010000057        22812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFRG010000057 DAZFRG010000000
VERSION     DAZFRG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.057"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..429)
                     /locus_tag="ACHKUC_001736"
     CDS             complement(34..429)
                     /locus_tag="ACHKUC_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO6618003.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMSIIAGLACVDGVFLEESLAQKAEYLWGYSADILVMGDDWAGKFDD
                     FSCICEVVYFPRTPSVSTTGIIEVIRGKA"
     gene            complement(432..866)
                     /gene="fliJ"
                     /locus_tag="ACHKUC_001737"
     CDS             complement(432..866)
                     /gene="fliJ"
                     /locus_tag="ACHKUC_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6618004.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSADAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQHESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(869..2209)
                     /gene="fliI"
                     /locus_tag="ACHKUC_001738"
     CDS             complement(869..2209)
                     /gene="fliI"
                     /locus_tag="ACHKUC_001738"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6618005.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSPEHQQSARAMKQHYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFAAIERFLRQEMHEPASLELVQSRLQILFPNGKKAEGQ"
     gene            complement(2202..2912)
                     /gene="fliH"
                     /locus_tag="ACHKUC_001739"
     CDS             complement(2202..2912)
                     /gene="fliH"
                     /locus_tag="ACHKUC_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6618006.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGCKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(2916..3926)
                     /locus_tag="ACHKUC_001740"
     CDS             complement(2916..3926)
                     /locus_tag="ACHKUC_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6618007.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            complement(3904..5550)
                     /gene="fliF"
                     /locus_tag="ACHKUC_001741"
     CDS             complement(3904..5550)
                     /gene="fliF"
                     /locus_tag="ACHKUC_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6618008.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVTAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMSNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(5555..5896)
                     /locus_tag="ACHKUC_001742"
     CDS             complement(5555..5896)
                     /locus_tag="ACHKUC_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6618009.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMYGVLGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(5911..6897)
                     /locus_tag="ACHKUC_001743"
     CDS             complement(5911..6897)
                     /locus_tag="ACHKUC_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO6618010.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLESTLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVTAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIFPDALGLPEIDVADHSELQWPVQPSVSVAETSDLG
                     QAGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            7284..8135
                     /locus_tag="ACHKUC_001744"
     CDS             7284..8135
                     /locus_tag="ACHKUC_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6618011.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSMPLTLN
                     VKIAELSLNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAR
                     KNENNHE"
     gene            8128..8499
                     /locus_tag="ACHKUC_001745"
     CDS             8128..8499
                     /locus_tag="ACHKUC_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6618012.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            8496..9248
                     /gene="fliP"
                     /locus_tag="ACHKUC_001746"
     CDS             8496..9248
                     /gene="fliP"
                     /locus_tag="ACHKUC_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6618013.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            9251..9523
                     /locus_tag="ACHKUC_001747"
     CDS             9251..9523
                     /locus_tag="ACHKUC_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO6618014.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIVQLHDLCIHLFTQAALLVH"
     gene            9525..10307
                     /gene="fliR"
                     /locus_tag="ACHKUC_001748"
     CDS             9525..10307
                     /gene="fliR"
                     /locus_tag="ACHKUC_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6618015.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            10297..11436
                     /gene="flhB"
                     /locus_tag="ACHKUC_001749"
     CDS             10297..11436
                     /gene="flhB"
                     /locus_tag="ACHKUC_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO6618016.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQQINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSNEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLGLQALPFKLAMNEGLSLYASVMRNFVILFIFFALLDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            11420..13513
                     /gene="flhA"
                     /locus_tag="ACHKUC_001750"
     CDS             11420..13513
                     /gene="flhA"
                     /locus_tag="ACHKUC_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6618017.1"
                     /translation="MAKTTKSFLAPLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGVGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFTALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            13592..13936
                     /locus_tag="ACHKUC_001751"
     CDS             13592..13936
                     /locus_tag="ACHKUC_001751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618018.1"
                     /translation="MIRRVAAHLAPVHNCRMRRERLIRPTGERGFVGMIRRVAAHLAP
                     MHNRRMRRERLIRPTDERGFVGMIRRVASHLASAYNCRMRRTCLIRPTGERGFVGMIR
                     RVCVASGIGAQL"
     gene            complement(13931..14428)
                     /gene="rayT"
                     /locus_tag="ACHKUC_001752"
     CDS             complement(13931..14428)
                     /gene="rayT"
                     /locus_tag="ACHKUC_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO6618019.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNVIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(14604..15353)
                     /gene="yafL"
                     /locus_tag="ACHKUC_001753"
     CDS             complement(14604..15353)
                     /gene="yafL"
                     /locus_tag="ACHKUC_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6618020.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            15665..16405
                     /gene="dpaA"
                     /locus_tag="ACHKUC_001754"
     CDS             15665..16405
                     /gene="dpaA"
                     /locus_tag="ACHKUC_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO6618021.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(16376..17143)
                     /gene="yafJ"
                     /locus_tag="ACHKUC_001755"
     CDS             complement(16376..17143)
                     /gene="yafJ"
                     /locus_tag="ACHKUC_001755"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO6618022.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNEAWQKIMPGEWRLFCLGECVV"
     gene            complement(17348..17926)
                     /gene="lpcA"
                     /locus_tag="ACHKUC_001756"
     CDS             complement(17348..17926)
                     /gene="lpcA"
                     /locus_tag="ACHKUC_001756"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO6618023.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            18166..20610
                     /gene="fadE"
                     /locus_tag="ACHKUC_001757"
     CDS             18166..20610
                     /gene="fadE"
                     /locus_tag="ACHKUC_001757"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO6618024.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(20653..21099)
                     /gene="ivy"
                     /locus_tag="ACHKUC_001758"
     CDS             complement(20653..21099)
                     /gene="ivy"
                     /locus_tag="ACHKUC_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO6618025.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            21280..22050
                     /gene="yafV"
                     /locus_tag="ACHKUC_001759"
     CDS             21280..22050
                     /gene="yafV"
                     /locus_tag="ACHKUC_001759"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO6618026.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVSWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(22101..22295)
                     /locus_tag="ACHKUC_001760"
                     /pseudo
     CDS             complement(22101..22295)
                     /locus_tag="ACHKUC_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(22321..>22812)
                     /locus_tag="ACHKUC_001761"
     CDS             complement(22321..>22812)
                     /locus_tag="ACHKUC_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO6618027.1"
                     /translation="VMLKMSLYVIILLFSLQFSAAITGKESEVVSPLLMDVNPSLTME
                     NISELSTSSEPSQQGVFPVICTRLHPGSVMKRQLLTGWGPVFIIGDDPFSLRWMSEHL
                     EILKSLNALGLVVNVESVERMEVLQQRADGLLLLPVICDNFVQALQLNAYPVLITEME
                     ISQ"
BASE COUNT         5028 a         6158 c         6156 g         5470 t
ORIGIN      
        1 ttactttgag gctcaaaaat aaattttatt ttgtcatgct ttccccctaa tcacctcaat
       61 gatccccgtc gtcgataccg acggcgtacg gggaaaatag accacctcac agatacacga
      121 gaaatcgtca aacttccctg cccagtcatc acccatcacc aaaatatccg cactgtaccc
      181 ccacagatat tccgctttct gcgccagcga ctcctccaga aatacgccat ccacgcacgc
      241 cagcccggcg atgatcgaca tgcggtcatc ctgatggtaa accggtgctc tgcccttttt
      301 ggcgatattt aaggcgtcgg acgatacgcc gaccagcagc cgttcgccca gcgtgcgggc
      361 gcgttgtaaa aggcgcagat gaccgacatg aaacacatcg aaggtgccga aggtgatgac
      421 cgttttcatg gctactccgc taatttgtgc cgtagccagc actgaacggc gatatcatcc
      481 accgcttttt gcgctttcgc cgtggcactt tcatgctgct cgcggcgttt gtcatccagc
      541 gtcagggcca ccactttctc ttcgcacgcc gccgccgtca ggtttttctg cattcgcgta
      601 gccttgatgt tagccagcgt tttttcctgc tcctgccagg cgatcacctt gcgtaaggtg
      661 cctttgtagc cgctgacgtt tttcagcgat tccaccgacg ccgccgcgtc cgcagacgtt
      721 ttctccacca ggtagcctaa cgctttgata ttgttgtcat agcccgcgca gatctgtttt
      781 tgctttgcca gttcgacggt tttgtctttg accgatttat cccgcaggcg ctgcaactgc
      841 gccagagtgt cgatgatttg tcgcatggtt attgcccttc tgcctttttg ccgttgggaa
      901 agaggatttg caggcggctt tgcaccagtt cgagggaagc gggttcgtgc atctcctggc
      961 gcaaaaatcg ctcgatagcc gcgaacattt tcaccgcttt gtcgacgctg gcgtcggcac
     1021 ctgccacgta gcccccgagc gggatcagcg gtttgatctc catataggcg gcgtagtgtt
     1081 gtttcatcgc acgggcggat tgctgatgtt ccgggctggt gacctggttc atgcagcggc
     1141 tgatcgactg gccgatatca atggccggat agtgccccgc ttccgccagt ttgcgggtta
     1201 atacgatatg tccgtccagc actgcgcggg cgcagtcgac tattggatct tgctggtcgt
     1261 cgccttccgc cagcacggtg tagatggcgg tcatcgatcc tgagctttcg ctgttgcctg
     1321 cgctctcgac cagtttgggg atcatgccga atgccgacgg cggatagcct ttggtggcgg
     1381 gcggttcacc gagtgagagg gcgatttccc gctgcgccat cgcgtagcgg gtcagggaat
     1441 ccaccagcag taaaacgtga tgcccgcggt cgcgaaacca ggcggcgatg gagtggcaca
     1501 gttcggtggc tttcaggcgc atcagcggcg attcatcggc aggcgcgaca acaacgatag
     1561 actttgccag cccgtccgcg cccagcgaat ggtcaataaa ctctttcact tcgcgcccgc
     1621 gttcgccaat caacccgacc accacaatgt cggctttggt ctggcgggtg atcatcccca
     1681 gcagcacgct tttcccgacg ccgctgcccg ccatcagccc gacacgctgg cctttgccga
     1741 tggtcagcag gccgttaatc gctttcacgc ccacgtccag cggttcgtcc actgaacggc
     1801 gggttaaggg attgaccgag ggtggcagcg gcggcagaag gtcgttgccg ttcaggcggc
     1861 ctttgccatc cagcggttcg ccgagaccgt taaccacgcg gcctaaccag ctttcgccaa
     1921 tcaggatgtc gtgggctttt tcttccggga agacgcgtgc gcctgccatc aggccgaccg
     1981 gctgtttaaa cggcattaag taggtgatgt cgcgattaaa gcccacggcc tgcgcgtcga
     2041 tcagcgtgtg attggcgctt tcgatgcggc acagttgccc ggtcattagc gggcagccga
     2101 cgctctccag cagaatgccg ttcaggcgca ccagccgccc ggcgacgcgg gccagcggga
     2161 tcgactccag cgagcgcagg gcgttatcga aagcagaaaa atcactcacc ctgcgactcc
     2221 ggcgttagcg tctcttgcag cgcggtcatg cactgttcaa ggcgatgctc acagccgata
     2281 tccagttcag atttgtcggt aatcactcgg cattcgcctg gcggcagatc cggcgaaggc
     2341 gtcagccccc attcgctgac tttttccggc accgcgtcgc gcaggcggtt aaactcttct
     2401 gtactcagca tcacttgcag ggtctccggc atcgccggga aggccgccag cgcctcttcc
     2461 accagcgcca gtaactgcgt cggttgcagc gccagttcgc agcggatcac ctgacgggtg
     2521 actttctcca ccagttgcag caaatcttcc cgctgtttac gttcaatatg cgcgaggaag
     2581 tcgttcactt taccggtgat ggcttccagc ggctgcgccg cctcaacaaa ctgtttgcat
     2641 ccttcctgct gcccggcgag gctgccttcg gtgtagccct gacgtcgccc ctcggcaaaa
     2701 ccttgttgat gaccttcgct aaagccctgc tcctgacctt cggtcattcc ctgagcaaac
     2761 cctttttgca gcccttcctg aaaaccgtcc atcaactgac gctggtaatc ggcggcgctg
     2821 atactggcag taagcggttc tccggtcacc gcacgctggc gcggcgggaa gcggtgcagg
     2881 cgatagcgcc cgcgcagtgt ttcaatggtc atgaattact ccgccgtccg ttccgcaaag
     2941 agctgtagct gaatttcgcc cgcttcttcc agctcacggg cgatgcccat gatttcgcgg
     3001 cggatctgtt cgatacggct gaccggcacc ggacccagac gcgcggtgat cgcttccagt
     3061 tgctgcgcct gacgttttgg cattaccgcg taaatcgagc ggcgcagcag ggcttcggtg
     3121 cctttcagcg ccaccgccca gtcttccatc ggcacttcgt caagcagacg ccgacgcact
     3181 tcttcgttct ggcggctgag gataaagaag tcgtacatct catcctggat ctgccccaac
     3241 acctcttcgt cacgttcgcg catctggtcg aggatcacct gctgattgcc ctggaagcgg
     3301 ttgacgatat ccgccgcctg tttgatgccc ttcaccttcg agccgtgctc cgaaagcacc
     3361 gacaggccac gttcaatcag gcggtccagc tcatccacca cgtcgcggtt gacgtcgttg
     3421 agtttcgcca cccggtagag aatttcgttt tgcaccgatt cgttgagata agagagcacc
     3481 gctgccgaga tttccggcgt cagaaacgcg aggaacaccg cctgcaattg cagatgttct
     3541 tcggaaatca gcatcgccaa ctggcgtggt tccacccatt gcaggcgcgc catccgcgag
     3601 cggatctcat cgccatagat gccgttgatc acgctactgg cgatctcggt gcccagcgct
     3661 ttgttaagaa tgccctgcaa catggagcgt gatgcgccgt tgatcccgct ctgttcgcgg
     3721 aactcatcaa agaagttatt gatcaccttg cgcgccatgc tggttttgac gccggaaagc
     3781 cgcgccatgt tttcgctcag gcgcaccact tcttcgcggc tgagtttttg catcaccgtc
     3841 gccgccgcct cttcgcccag acacagcaac aggatcgccg cctgttccag gtagctgttg
     3901 ctcttatttg ttgtcaattc gctcattgct ggtgatccat tgtctgagaa cttcggctac
     3961 gcgatcggtg tcgctcatgg ccagtttttg caggaactcg agtttcactt cgaggccaga
     4021 actttgtgac ggcaggctgt catcgccggg tagcgacggc aggtcgacgt ttttgcgctc
     4081 ctcttccggc atggcaatcc acggttcact gacggcttcc ggttctggtg tgataaccgg
     4141 cgcacgtacc gccgtcagcc gtttcatcaa cggacgcacc acgaaaaaca gcagcaacag
     4201 cgccagcagg ccgcagccaa tcagccgcac ccaggccagt acgctgtcat ctttccacag
     4261 tggaataacc ggttcgacag gcaccgcctg cggtacaaag ttgagtaacg ataaggagag
     4321 attgtcacca cgctgggcgt cgatcccggc ggcattgttc agcaacgcgg tcagttgcgt
     4381 ggtttgctcc ggcttccagt ttttcagcgc cggagcgttt tgattgagta ccaccgccac
     4441 gttgaggcgt ttcaccgcga agccgggatg ctgaatatgc tggacgctgc ggtcgtaaga
     4501 gtagtcgcgc ttactttcgc tgtgtttgga aagcgcttcc ggcgagcggt tttcttccgt
     4561 accgttgctc atctgattcg ctgcaatcgg cggacggtta ctgagcgaac caggcacgcc
     4621 catcgccacc tgattggtgt cgctgtccag cacgctctct tcgcggttga ttttcggcgc
     4681 gtcaccgtag tgttcctggg tttcgtcgat ggtgctgagg tcgagatccg gcatcacact
     4741 gacgcggtaa ttccccgtgc caaccagcga atccagcacg ttcgccacgc tggcacgggt
     4801 tttgtcctga atatctttga ggatctgatc gcgtttacgg gtcgcggcag aaaccgcctc
     4861 gcctgcgcca atgccgtcgg tcagcagatt tcccgcctga tcgaccacgc taactttgct
     4921 ggcctgtagc cccggaatac tgccggacac cagatgcaca atggcggtca cctggtccat
     4981 attcagcttc gcgccgtaat gcaagcgcac caccaccgag gcgctgtttt gcggctcatc
     5041 gctgaccaca aacgagcttt cttcgttgag cgccagatgc acccgcgcgc tttccaccgc
     5101 gtccagcgac atgatgctct gcgccagctc cccttcgaga ctgcgtttat agcgcacgtt
     5161 ctgcacgaac tgactgctgc cgagcacttc gtctttgtcc atcagctcgt agccgctggg
     5221 caatatcgcc tgcacgcctt tcgcggcaag ggtcattcgc gttttcgaca gtgcatcttc
     5281 cggcaccaga atctgcccgc tctgcgggtc gatgcggtag gtgagctttt cgccgtccag
     5341 caccgtgaca atctgcgaca cgggcagatt ttcctggcgt ccgtagagcg aaacgtagcc
     5401 gcgattaccg gtccacagca cgctgacgat aatcgccgtc gccgccacgg caagccccac
     5461 tcccgccatc aacgcccagc gtttgttatc tgcaagacgc agtcgaaacg ccggaaacgc
     5521 ctgggttaac ttctttattt gtgcattcat agggttagat cgacatactc atcaggtcgt
     5581 taaacccggt ggcgatttta ttgcgcacct gcaccagcgc ggagaaagag agcgacgcct
     5641 gctggctggc gatcatcgca cctgccaggt catcactttt gcccgtgtcg acagccgttt
     5701 gctgctgctc cgccacctgc tggaactgat caacgtgacc aagcacgccg tacataatgc
     5761 ggttaaacga cacgtccggg tctgtggaac tgaaggtcat ggcgggtagc accggcgcct
     5821 gggcgttggc ctgcatccgc tgcacgtcct gcatgatttg cgcctgcatg gtgctgttga
     5881 ttgtggtaat gctcattttt aacttccggg tcaggagaaa acttcgatac cttgcttgcg
     5941 catggaggcc agccgatagc gcagtgcgcg cggggtaatg cctaacaggt cggcaatttt
     6001 gctgcggttg ccctgatatt tacgcatcag gtcggcgata tattgatact gggcgctacg
     6061 tccggcctgg cctaaatcgc tggtttccgc aacagatacg gagggctgaa caggccattg
     6121 cagttcactg tgatcggcaa cgtctatttc cggcaggcct aaggcatccg ggaaaatcac
     6181 gccgtcgcgg tttaagatca ttcctcgctg aatggcgttt tccagttggc gaacatttcc
     6241 gggccagcgg taattaagta gcgcccggcg ggtagattct gaaagggtga tatttttcac
     6301 cagcaccgtg gcatattttt taataaacga ctctgccagc ggaataatat cgttcagacg
     6361 ttcacgcagt ggcggcatgg ttataggaat aaccgccaga cgataataaa gatcttcacg
     6421 aaaacgtcca gcagtcacat cctgttccag atttttattg gtgcaggcaa tcaggcgaaa
     6481 attgagtttg atttgtcggt tgctgcctaa tcgctcaacc tgctgttcct gcaatacgcg
     6541 taatattttg gcctgtaatg ccagcggcat atcgccaatt tcatcgagca ataaggtgcc
     6601 gttattagcc agttccattt ttcccggcac gctggcaatt gcgccggtaa atgcaccttt
     6661 gtcgtagcca aataaggtcg attccagcat attttccggg atcgccgcac agttcacgcc
     6721 gatatagggc gcgttatcat tttcaccaaa ggctacggtg tgaatatatt tcgccacgca
     6781 ctctttcccc gcgccggttt cgccctgaat gagcaccggt acgttaaagg cggcgacacg
     6841 ctttgccaga gtaaaagcgt taatgctgga agcggcagtt gcaataagtt cagacatagg
     6901 gtgaacgata taagtagcga caataatcag gatgttctga agcgtatcac cggtgggatt
     6961 aacaagttcg aattaaacga taataaaaat attatttgtt tataaacaac tagttggcaa
     7021 tgtataccgg ataaggcgtt tacgccgcat ccggcaattg cgtgccgatg cctgatgcga
     7081 cgctgcgcgt cttatcaggc ctacagtttg tgcaatgttt tatgacgatg cccgatatct
     7141 cgcccgttaa taatttaatt attttttcac cctattaata atttgcccct ttcactcctc
     7201 ctctatttaa tacctcacct tgtgtcaccg ccggatttgc gtgcacaaaa tcgcccatac
     7261 cattacagca gagaaagtaa gccatgctga agtacagtaa aacgccaggc atctttaaat
     7321 tagaaggtaa tcggctcgga cgcccctacc atcgcctgcc gaccctgttt acggggaatt
     7381 ttgacgtgat tgattcgcat cttggcagct actttctcaa aaagcatcgc agcaatatca
     7441 ccctaaaaaa gattggctgc gaaatggata ttattaataa aaacgctgaa ctgatggttt
     7501 cccaggtcgg gcatctggcg tttgatattg atcgctcctt attgttaatg ctgctgggca
     7561 atttttatgg tctggagtcg tctctcgaag aggctaaagc ccataacggg ttgccgacca
     7621 aaaccgaaac acgattaaag aaccgtctgg cgctggatat ctgcacgctg atatttaatc
     7681 tgcaaacctc cggtattgcg ctgaaattaa aactggacag cagcacggta attacccact
     7741 gggcgtatca attaaccttt acccttgcgg gcgatgagga gagcagtttt cgtattttgc
     7801 tcgacgacgc ccacaccgat tttattttga atctgatccg ccacagcgag cacagtaaac
     7861 cgcagcaggt ggcgaaatcc gttaataaac cggccctgat taaagaaatc atccgctcta
     7921 tgccgctgac cctgaacgtc aaaatcgccg agctgtcgct gaatgtcgcg gatctggcgc
     7981 agataaaagc gggggatatt ttacccatct cgctgggaga aacgttcccg gtggcgattg
     8041 ggcaatctga attatttagc gccctgattg tggaagacaa agacaaactg tttttgtcgg
     8101 agctggcgag aaaaaatgag aacaaccatg agtaagcaag aagatatttt agccggggat
     8161 tttggcctca ccgatgaagt tgcgcctgtc gccaaatccg ccgacgccga aacgctggtc
     8221 acccgcctgg aagaccgatt ttccgactcg atgacgctac tgaaacgcat tccggtgacc
     8281 ttaacgctgg aagtgtcgtc ggtggaaatc atgttagccg acctgcttaa catcgacgac
     8341 gacacggtga ttgaactgga taaactcgcc ggggagccgc tggatattaa agtgaacaat
     8401 atcctgctgg gcaaagcgga agtggtggtg gtcaacgaga agtacggcct gcgcgtgctg
     8461 gaatttaaca cccgcgatat caacgacctg gcaccatgaa acgccgtact caactgacgc
     8521 tgggcctggc gctgctggcg ctcgccccgc tggcgctggc ccagggtggc gatattgcgc
     8581 tgcttaacgt ggtgacccac ggcaacactc aggagtacag cgtcaaaatt caggtactga
     8641 ttctgatgac cctcgtcggt ttgctgccga cgatggtgct gatgatgacc tgctttacgc
     8701 gctttattat cgtgctgtcg ctgctgcgcc aggcgctggg gctgcaacaa acgccgccga
     8761 accgcattct gataggtatt gcgctctcac tcaccctgct ggtaatgcgc cccatctggc
     8821 tcaatattta cgaccacgcg gtagtgccgt ttgaaaacga tcagattacc ctgaccgatg
     8881 cgttaagcac cgccgcgacg ccgctgaaac gctttatgct ggcgcagacc gacaaaaaag
     8941 cgatggcgca aatcatgacc atcggcggcg cgaagggcaa tgccgccgac caggatctct
     9001 ccatcgtggt gcccgcctat gtactgagcg agctgaaaac cgccttccag attggcttta
     9061 tgatttacat cccgtttctg gttatcgacc tgattgtcgc cagcgtattg atggcgatgg
     9121 ggatgatgat gctgtcgccg ctgattgttt cgctaccctt taaactgatg ctgtttgtgc
     9181 tgattgacgg ctggtcgctg accatcggca cgctcaccac cagcattcgc gggctggggc
     9241 tgggctgatt atgttgaccg tagacgtagc cgccgacatt gtcgccagcg gtattaaagt
     9301 agtgattttg ctggtttcgg tactggtggt gccaagcctg ctggtcggcc tgctggtgag
     9361 cgtgttccag gcggtgacgc agattaatga acagaccctg agctttttgc caaggctgat
     9421 tgtcacgctg gtggtgctgg gcgtgtgcgg aaaatggatg attgtccagt tgcatgacct
     9481 gtgcattcac ctgtttaccc aggccgccct gctggttcat taagatgcgc acaagcgacg
     9541 ttacgcaact gatggatctg gcgctggggc tgtggtttcc cttcgtgcgc attatggcgt
     9601 ttctgcgcta tatgccggtg ctggataaca gcgccctgac cgcgcgcgtg cggattattt
     9661 tgtcgctggc gctggcgatc atcatcacgc cgttgatccc ccatcccatt ccccacgacc
     9721 tgctttcgct caatagcctg atcctgacgg tagagcaaat tttatggggc atgttattcg
     9781 ggctgatgtt tcagtttctg tttctggcgc tacaactggc cgggcaaatt ctctcgttca
     9841 atatggggat gagcatggcg gtgatgaacg acccgagcag cggcgcatcg accaccgtgc
     9901 tggcggagct aattaacgtc tacgcgatcc tgctgttttt tgccatggac gggcatctgc
     9961 tgctggtaag cgtgctgtat aagggtttca cctactggcc gatcggcaac gcgctgcatc
    10021 cgcaaaccct gcgtaccatc gcactggcct ttagctgggt gctggcgtcg gcatcgctgc
    10081 tggcgctgcc gaccactttt atcatgctga ttgtgcaggg ctgctttggc ttgctcaacc
    10141 gtattgcgcc gccgttgaac cttttttctc ttggctttcc catcaacatg ctggcggggc
    10201 tggtctgctt cgccaccctg ctctacaacc tgccagatca ctatctgcat ctggcgaatt
    10261 ttgtgttgca gcaactcgac gcgctgaaag gtcactatgg cggatagcag cagcgaagaa
    10321 aaaacagaga aaccctcggc gcaaaagctg cgtaaagcgc gtgaagaggg gcagctaccg
    10381 cgctcgaagg atatggggct ggcagcgagc ctgtttgcgg cgtttgtggt gatctccagc
    10441 agtttcccgt ggtatgccga ttttgtgcgc gagagtttta tcagcgtgca tcagtacgcg
    10501 cagcagatta ataacccgca ggtgatcggc cagtttctgc gccatcacct gttaattctc
    10561 ggcaagttta tccttacgtt gctgccgatg cccgccgccg cactgctctc ctcactggtg
    10621 cccggcggct ggctgttttt gccgaaaaaa atcctcccgg atttcagcaa aatcagcccg
    10681 ttaaaaggca ttgggcgact gttttccaac gaacatctgg cggaaacggg gaagatgaca
    10741 gtgaagtcgg tggtggtgct ggtaatgctg tgggtaagcc tgcgcaataa ctttgccgcc
    10801 tttctcggcc tgcaggcgct gccgttcaaa ctggcgatga atgaggggtt atcgctctac
    10861 gccagcgtga tgcgcaactt tgtcatcctg tttatcttct ttgcgcttct cgacgtgccg
    10921 ctggcgaaag ccctgttcac caaagggctg aagatgacca aacaggagct gaaagaagag
    10981 tacaaaaatc aggaaggcaa gccggaagtg aaggcgcgcg tgcggcggct gcaacggcaa
    11041 ctggcgatgg ggcaaattcg caaagtggtg ccgaaagcca acgtggtgat caccaacccg
    11101 acccactacg ccgtggcgtt acagtatgac tcatcacgtg ccgccgcgcc gtttgtggtg
    11161 gcgaaaggca ccgatgaaat cgccctctat atccgccagg tcgccactga aaatcaggtg
    11221 gaagtggtcg agttcccgcg actggcgcgc tcggtttact acaccacgca ggtgaaccag
    11281 caaattccgt ttcagcttta ccgggccatc gcccacgttc tgacctacgt cctgcaaatg
    11341 aaacactggc gcgagggcgc acagccgcgc ccggccctga acagacatat ttccattccc
    11401 aaagaggtac ttaaactgga tggcgaaaac aactaaatcg ttcctcgcgc cgctgcgcgg
    11461 cggcaatctc ggcgtgccgt tggtgatcct gtgtattctg gcgatggtta ttctgccgct
    11521 gccgcctgcg ctgctggata ttctgttcac cttcaacatt gtgctggcgg tgatggtgct
    11581 gctggtggcg gtgtcagcga aaaggccgct ggaattcagc ctgttcccga ccattttgct
    11641 tatcaccacc ttaatgcgcc tgacgctcaa cgtcgcctcc acgcgcgtgg tgctgctaca
    11701 tggccatctc ggcgtgggcg cggcgggtaa ggtgattgag tcgtttggtc aggtggtgat
    11761 tggcggcaac tttgtcgtcg gtttcgtggt gtttatcatc ctgatgatca tcaactttat
    11821 tgtcgtcacc aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc
    11881 gatgcccggt aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc
    11941 gcaggcacaa acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcaatgga
    12001 cggggcatcg aagtttgtgc gcggggacgc catcgccggg atgatgattc tggcgatcaa
    12061 cctgatcggc ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt
    12121 ccagcaatat gtgctgatga ctatcggcga tgggctggtg gcgcaaatcc cttccctgct
    12181 gctctccacc gcggcggcga ttatcgtcac ccgcgtcagc gataacggtg atatcgccca
    12241 tgacgtgcgt aatcagttgc tggcaagccc gtcggttctc tacaccgcca ccgggatcat
    12301 gtttgtgctg gcggtggtgc cggggatgcc gcacctgccg tttttgctgt tcaccgccct
    12361 gcttggtttt accggctggc ggatgagcaa gcgcccacag gcagcggagg cggaagagaa
    12421 aagcctcgaa acgctaaccc gtacgatgac agaaaccagc gagcaacagg tcagttggga
    12481 aactattccg ctgatcgaac ctatcagttt aagcctcggc tacaaactgg tggcgctggt
    12541 ggataaagcg cagggcaacc cgctcaccca gcgtattcgc ggcgtacggc aggtgatttc
    12601 cgacggcaac ggcgtgctgc tgccggagat ccgcattcgg gaaaatttcc gcctcaagcc
    12661 cagccagtac gccattttca tcaacggcat taaggctgat gaagcggata ttccggcaga
    12721 taaactgatg gccctgccct ccagcgaaac ctacggcgag attgacggcg tactggggaa
    12781 tgacccggcg tacgggatgc cggtcacctg gattcagccc gcgcagaaag cgaaggcgct
    12841 gaatatgggg tatcaggtga tcgacagcgc cagcgtaatt gccacccatg tgaataagat
    12901 tgtgcgcagc tatattcctg atttgtttaa ctatgacgat attactcagc tgcataaccg
    12961 tttgtcgtcg atggcaccgc gactggcgga agatttgagc gcagcgctca attacagcca
    13021 gttgctgaaa gtgtaccggg cgctactaac tgaaggcgtt tccctgcgcg atatcgtcac
    13081 catcgccacc gtgttggtcg ccagtagtgc ggtgactaaa gatcatattc tgctggcggc
    13141 cgatgtgcgc ctggcgctgc ggcgcagcat aacccatccg ttcgttcgca agcaggagct
    13201 gacggtgtat acgctgaata atgagctgga aaatctgctg accaacgtgg tgaatcaggc
    13261 gcagcagggc gggaaagtga tgcttgatag cgtgccagtg gatccgaata tgctcaacca
    13321 gttccagagc acgatgcctc aggtgaagga gcagatgaaa gcggcgggga aagatccggt
    13381 gctgctggtg ccgccgcagc tgcgcccctt gctggcgcgc tacgcaaggt tgtttgcgcc
    13441 ggggctgcat gtgctgtcgt ataacgaagt gccggatgag ctggagttga agataatggg
    13501 ggcgttgatg taggcatccg gcatcggtgc atattgccgg atgcggcgtg aacgccttat
    13561 ccggcctacg ggtgagcacg gttttgtcgg catgataaga cgcgtagcgg cgcatctggc
    13621 accggtgcac aactgccgga tgcggcgtga acgccttatc cggcctacgg gtgagcgcgg
    13681 ttttgtcggc atgataagac gcgtagcggc gcatctggca ccgatgcaca accgccggat
    13741 gcggcgtgaa cgccttatcc ggcctacgga tgagcgcggt tttgtcggca tgataagacg
    13801 cgtagcgtcg catctggcat cggcgtacaa ctgccggatg cggcgtacat gccttatccg
    13861 gcctacgggt gagcgcggtt ttgtaggcat gataagacgc gtctgcgtcg catcaggcat
    13921 cggcgcacag ctataaaatg atacgctccc cagcactaaa atccgttacg tctcccgccc
    13981 aatcgatggg atataaccct ttcgcaacat cgcggtgaaa cgttgagaat ggccaatcac
    14041 tcacttgttt tacccaacca tgttttactg gatttatata aatataatca acatgatggc
    14101 gatagtcttt ggtattgcgg atagcatgtt cccaaaaacg tggttgccag atatttttca
    14161 atccacaagc atgggtaaat tgctttttaa tttcccgcca gcgcgaggaa aaatcatcat
    14221 cgccttcagg taatgtccat atgcaatgca tatgctccgg caaaactacc caagcgttaa
    14281 tttcaaaagg tctgtctcgc ttaactttaa taataacgtt acggagcgtc tgaaactggg
    14341 tggtcaaaag ttggtttcga cgattctgta aatttaccgt gaaaaaccag gttcccccct
    14401 tgatgtaata acgacgatat tcagacataa cgactccttt ttctcaacgg taagaagaaa
    14461 aggaaaagtc ggcaatgatg ttgtgttaaa tcgggaaagc ggctcgcaaa aaatgcacag
    14521 atttgtaggc atgataagac gcgtagcgtc gcatcagaca ccggcgcaca accgccggat
    14581 gcggcgtgaa cgccttatcc ggcctacaaa atcgtctctt ccgtcaaaat ccgccgcgcg
    14641 cctaaaaagt gatcctgcca gaaaggttcg gctaaccgac ttacccgaat ggtttccccg
    14701 gtgcgcggtg actcgataaa ctgaccgtct ccaagatata cgcccatatg gtcggctatc
    14761 tcacgactgt ggatatggaa aaacagcaaa tctccccggc gcaggtcgtt attcgccaca
    14821 atcgttgccc gacggtagtg gtacatctca ttggccgtgc gcgggagctt agcctcaagg
    14881 atcttgttgt aggcataaaa aaccaacccg ctacagtcga agcctttatc aggccgcgtg
    14941 cccccccaga tataaggctt acctagctgc tgctccaggc ggtgaatcgc cacttcggtg
    15001 atgttatgta gatggtcgct gcttaagaaa tgattattct ccaccaacgc ctgccagcgt
    15061 ctgtcactcg ccttgagcgg cgcaggaaac cattctggat gctgttttat ctgctcccga
    15121 ttatgctggc gcagcgccct tctgcttttg gcattgcctt ccacaatata gctggcctgc
    15181 tttttcagat gggtttggta ctgttttaat aaacgcgcac ggttttgcat ccgctggcgg
    15241 gcggcgtagc taaaagagat atgtgatgtg gtggcgctgg cgagtaaagg gagcaaaacg
    15301 cataacgaaa acacgcctgg atgcagaaat ctgccgtgca agaacgaaga catgaaggac
    15361 ataaaccagc tgaatgaagg tgagaatttt aaaagaccgg aggagtaaac gggattatta
    15421 attgcttatt tgcatattac tattgcggtt tcataatctt aggaaataag aaataaatct
    15481 acaatccatg cgcttttcgg actcttgaaa actgcccctg tgctaaattt gtaaaccctg
    15541 cgccgacagg cttgcgtaaa actcttttgg tgataatgtg agccttccaa atccagacta
    15601 ttccgatttg ggattcattt tgccgtcctg gcgtgtgtaa ccgttttatc aaggaataag
    15661 cagtatgcgt aaaatcgcat taattcttgc gatgttgttg atcccgtgcg tttcgtttgc
    15721 cggtttgctg ggcagcagta gctcgaccac gccagtgagc aaagagtata agcagcagtt
    15781 gatgggatcc cctgtctaca tccagatctt caaggaagaa cgtacgctcg atctctacgt
    15841 caaaatgggc gagcaatatc aactgctcga cagctataaa atctgtaaat attccggcgg
    15901 cttggggcca aaacagcgtc agggcgattt caaaagcccg gaagggtttt atagcgtcca
    15961 gcgtaatcag ttaaaaccag acagccgtta ctacaaggcg attaatatcg gtttccccaa
    16021 tgcctatgac cgtgcgcatg gttatgaagg gaaatacctg atgatccacg gcgattgtgt
    16081 ttccatcggc tgctacgcga tgaccaatca gggtattgat gagatcttcc agttcgttac
    16141 tggtgcgctg gtttttggtc agccgagcgt gcaagtgagt atttacccgt tccgcatgac
    16201 cgacgccaat atgaagcgcc ataaatattc caactttaag gacttctggg agcagctgaa
    16261 gccaggttac gactactttg agcaaacccg taagccaccg accgtttctg tggtcaatgg
    16321 ccgttacgtg gtcagcaagc cgttaagcca cgaagttgtg cagccacaac tggcatcaaa
    16381 ctacacactc cccgaggcaa aataagcgcc attcacctgg cataatcttt tgccaggctt
    16441 cattgcccgt cagcggctgt gtcgcaatca ccgtgaccac atcattcggt gtggtctgcg
    16501 agctgaagtc gatttccaca tcctgatcca gcaacgttgc cacgccaaac ggcgcgcggc
    16561 gggtgatcca gtgtaaatta gtcgagcaat acgccattac atagcgcccg tcagaaagca
    16621 gcatgttgaa aacgcccttc tgccgcagtt catccgccag tgaggcgata tatttaaata
    16681 ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt taatttatgc aggagccagc
    16741 aaaaggcttt ttcgctatcg gtttcaccga ccgggcggaa gttgccggtt tccagtgatt
    16801 tgtagcccgt cagttgtcca ttatgggcat aagtccagtt acgcccccat aactcgcggg
    16861 taaacgggtg agtattttcc agcgccacct cgccccgatt agcctggcga atatgagcca
    16921 ccaccgaaca ggattttatc gggtagtcct ggacaagttt ggcgatgggg gaattaaagc
    16981 tgggttgcgg atctttaaat gtgcgacagc ctttaccttc gtaaaaggta atgccccagc
    17041 catctttatg tggcccggtt ccaccaccac gctgtacaag tccggtgaaa ctaaagcaga
    17101 tatcggtagg gacgttggca ctcatcccga gcaattcgca catgggtaac ctccacaaca
    17161 gcgggagcgc atggcccccg gcgaagattg atttaagaat tttcaggtcg gatgtggcgt
    17221 gaacgcatta ttcggcctac ggttcgggtg cagattgtag gcatgataag acgcgtcagc
    17281 gtcgcatcag gcatcagagc acaaatgccg gatgcggcgt aaacgcctta tccggcctac
    17341 gccagactta cttaaccatc tctttttcaa ttaactggat caggatatgg atcactttaa
    17401 tgtggatctc ctgaatgcgg tcggcataac caaagtgcgg aacgcgaatt tcaatatccg
    17461 ccgtgccagc cattttgccg ccgtctttac ccgtcagggt gatcactttc atccccttct
    17521 cacgcgccgc tgcgatcgct ttgatcacgt ttgcagagtt accggaggtg gagatcccca
    17581 gcagtacatc gccttcgcga cctaccgctt caacgtaacg ggagaaaata tcattgaaac
    17641 caaaatcgtt accgacgcag gaaatatgac taacgtcaga aatggcaatc gccgggtagc
    17701 ccggacggtt ttcacggtag cgaccggtca actcttcggc aaagtgcatg gcgtcgcaat
    17761 gggagccgcc gttgccacag gaaagcactt tgccaccggc tttaaagctg tctgctaaca
    17821 ggaccgccgc gcgctgaatg gcgtgaatat tggcgtcatc ttttaaaaag ttagccagcg
    17881 tttccgccgc ttcgttcagt tcattacgaa taagatcctg gtacatgagg atatccttca
    17941 gcataaatgt aatagacaaa atgcagtgta ccggataccg ccaaaagcga gaagtacggg
    18001 caggtgctat gaccaggact ttttgacctg aagtgcggat aaaaacagca acaatgtgag
    18061 ctttgttgta attatattgt aaacacattg ctaaatgttt ttacatccac tacaaccata
    18121 tcatcacaag tggtcagacc tcctacaagt aaggggcttt tcgttatgat gattttgagt
    18181 attctcgcta cggttgtcct gctcggcgca ttgttctatc accgcgtgag cttatttatc
    18241 agcagtctga ttttgctcgc ctggacagcc gtcctcggcg ttgctggtct gtggtcggcg
    18301 tgggtactag tgcctctggc cattatcctc gtgccattta actttgcgcc tatgcgtaag
    18361 tcgatgattt ccgcaccggt atttcgcggt ttccgtaagg tgatgccgcc gatgtcgcgc
    18421 actgagaaag aagcgattga tgcgggcacc acctggtggg agggcgactt gttccagggc
    18481 aagccggact ggaaaaagct gcataactat ccgcagccgc gcctgaccgc tgaagagcaa
    18541 gcgtttctcg acggtccggt agaagaagcc tgccggatgg cgaatgattt ccagattact
    18601 catgagctgg cggacctgcc gccggagtta tgggcgtacc ttaaagagca tcgtttcttc
    18661 gcgatgatca tcaaaaaaga gtacggcggg ctggagttct cggcttatgc ccagtctcgc
    18721 gtgctgcaaa aactctccgg cgtgagcggg atcctggcga ttaccgtcgg cgtgccgaac
    18781 tcattaggcc cgggcgagct gctgcaacat tacggcactg acgagcagaa aaatcactat
    18841 ctgccgcgtc tggcgcgtgg tcaggagatc ccttgctttg cactgaccag ccccgaagcg
    18901 ggttccgatg cgggcgcgat tccggatacc gggattgtct gcatgggcga atggcagggc
    18961 cagcaggtgc tgggaatgcg tctgacctgg aacaaacgct acattacgct ggcaccgatt
    19021 gcgaccgtgc ttggtctggc gtttaaactc tccgacccgg agaaattact cggcggtgca
    19081 gaagatttag gtattacctg tgcgctgatc ccgaccacca cgccgggcgt ggaaattggt
    19141 cgtcgccact tcccgctgaa cgtaccgttc cagaacggac cgacgcgcgg taaagatgtg
    19201 ttcgtgccga tcgattacat catcggcggg ccgaaaatgg ccgggcaagg ctggcggatg
    19261 ctggtggagt gcctctcggt aggccgcggc atcaccctgc cttccaactc aaccggcggc
    19321 gtgaaatcgg tagcactggc aacgggtgcg tatgcgcaca ttcgccgtca gttcaaaatt
    19381 tctattggta agatggaagg gattgaagag ccgctggcgc gtattgccgg taatgcctat
    19441 gtaatggatg ctgcggcatc gctgattacc tacggcatta tgctcggtga aaaacccgcc
    19501 gtgctgtcgg ctatcgttaa gtatcactgt acccaccgcg ggcagcagtc gattattgat
    19561 gcgatggata tcactggtgg caaaggcatt atgctcgggc aaagcaactt cctggcccgt
    19621 gcttaccagg gggcaccgat tgccatcacc gttgaagggg caaatattct gacccgtagc
    19681 atgatgattt tcggtcaggg ggcgattcgt tgccatccgt acgtgctgga agagatggaa
    19741 gcggcgaaga acaatgacgt caacgcgttc gataaactcc tgtttaaaca tatcggtcac
    19801 gtcggtagca acaaagttcg cagcttctgg ctgggcctga cgcgcggttt aaccagcagc
    19861 acgccaaccg gcgatgccac taaacgctac tatcagcacc tgaaccgcct gagcgccaac
    19921 ctcgccctgc tttctgatgt ctcgatggct gtgctgggcg gcagcctgaa acgtcgcgag
    19981 cgtatctcgg cacgtctggg ggatatttta agccagcttt acctcgcctc tgccgttctg
    20041 aagcgttatg acgacgaagg ccgtaatgaa gccgatctgc cgctggtgca ctggggcgta
    20101 caggatgcgc tgtatcaggc agaacaggcg atggacgatt tactgcaaaa cttcccgaac
    20161 cgcgtggttg ccgggctgct gaatgtggtg atcttcccga ccggacgtca ttatctggca
    20221 ccttctgaca agctggatca taaggtggcg aagattttac aagtgccgaa cgccacccgt
    20281 tcccgcattg gtcgcggtca gtacctgacg ccgagcgagc ataacccggt tggtttactg
    20341 gaagaggcgc tagtggatgt gattgccgcc gacccgattc atcagcggat ctgtaaagag
    20401 ctgggtaaaa acctgccgtt tacccgtctg gatgaactgg cgcacaacgc gctggcgaag
    20461 gggctgattg ataaagatga agccgctatt ctggtgaaag ctgaagaaag ccgtctgcgc
    20521 agtattaacg ttgatgactt tgatccggaa gagctggcga cgaagccggt aaagttgccg
    20581 gagaaagtgc ggaaagttga agccgcgtaa ttgaataacg gagccgaaag gctccgtttc
    20641 tttatccgct aattatttaa aattaaagcc atccggatgg ttttccaggc tgccggtcaa
    20701 cgccgcgaac agcaccgttt taccatcaat cgaaagcgca tcgttcacat tcaaccaggt
    20761 gagtttctct tgcgacgttt tctcatcaat agtcgagaac agccccgtca tctgattaga
    20821 tttctcggac cacatcacag cgatacgttg cgagccacag tcatgcggtt tgcacgcgct
    20881 catcacctga tacgtctcat ctcccaatgt cacggtttgt gcgggagtat aagtaccgcc
    20941 tttcatcacc caggcaggca gcttatgccc ttgcaccatc tgattaaacg ccgctttggt
    21001 ggtttcgccc tttgcaagac tgctaatggt taaatcatcc tgcgccattg cactggtggc
    21061 gatgaccaga gcggcgactg tcgttattgc cttaaacatc attcctcccg ggcttatcct
    21121 gcccatgtga ttaactccct aaagcctggc gataacacga taatttgcaa atcagatatt
    21181 tccaatatca gcatttgtta tcaatttatt ctgcgctaag gcaactgcct gataaggtgt
    21241 tagagttata aaacccttcg cataaaggag ttgtacaccg tgcctggttt gaagattacg
    21301 cttttgcagc aaccactggt gtggatggat ggtcctgcca acctgcgcca ttttgatcgt
    21361 caactggaag gtattaccgg gcgcgatgtg atcgttctac cggagatgtt taccagcggc
    21421 tttgccatgg aagcggcagc ttcgtcgcta gcacaagatg acgtagtgag ctggatgacc
    21481 gctaaagcgc agcagtgcaa tgcgctgatt gcaggcagtg ttgcattaca aacggagtct
    21541 gattcggtta accgcttttt gctggttgag ccgggcggca cggtacattt ttatgataag
    21601 cgtcatctgt tccgcatggc agatgagcat ctgcattata aagcgggcaa tgagcgagtg
    21661 attgtggaat ggcgcggctg gcgtattttg ccgctggtgt gctacgactt acgttttccg
    21721 gtgtggtcgc gcaatctcaa cgattatgac ctcgccatct acgttgccaa ctggcctgca
    21781 ccgcgttctc tgcactggca ggcattgctg acagcccgcg cgattgagaa tcaggcgtat
    21841 gtagcgggat gtaatcgcgt cggcagcgat ggcaacggct gccattatcg cggtgacagc
    21901 cgggtgatta atccacaagg cgagattatc gctactgccg acgcgcatca ggcaacgcgc
    21961 attgatgcgg agctatcgat gatggcattg cgggaatata gagaaaagtt tccggcatgg
    22021 caggatgcgg atgagtttag gttgtggtga gtgggggttg tgtttagggc cggggagagt
    22081 cggggaagac tacgaaagcc cgctctcctg cgacgacttt tatcacagga gtgtggaagt
    22141 gcttttccat caatcgcaat gacgtctatg tcgtctgaag aatgacattc acgcatccgg
    22201 ttaataaagc attcgtgaaa ttttactgaa cggatacagg acataactct gattatggtg
    22261 tcgtgaacag gattaccatt ttcaaaatca ccatattatt agcagtaatt atgttattaa
    22321 tcattgtgat atttccatct ctgtgattaa taccggataa gcatttaatt gcagagcttg
    22381 cacaaaatta tcgcagataa ctggcaataa caataaccca tcagcccgct gctgtaatac
    22441 ctccattcgt tctactgatt caacattaac aaccaaacca agggcattta gcgatttaag
    22501 gatctccaga tgttcactca tccagcgcag agaaaaagga tcatcaccaa ttataaaaac
    22561 aggcccccag ccggttaata actgacgttt cattacggag ccaggatgaa gtcgagtaca
    22621 tattacagga aataccccct gttgcgatgg ttccgatgat gtggataatt cagaaatatt
    22681 ttccattgtt agtgaaggat taacatccat taataatggt gaaacaacct cactttcctt
    22741 gccagtaatg gctgcactaa actgaaggga aaataacaat attattacgt ataaactcat
    22801 ttttaacata ac
//
LOCUS       DAZFRG010000058        22574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFRG010000058 DAZFRG010000000
VERSION     DAZFRG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.058"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..1011
                     /gene="ybtA"
                     /locus_tag="ACHKUC_001762"
     CDS             52..1011
                     /gene="ybtA"
                     /locus_tag="ACHKUC_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO6618028.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            1202..7309
                     /gene="irp2"
                     /locus_tag="ACHKUC_001763"
     CDS             1202..7309
                     /gene="irp2"
                     /locus_tag="ACHKUC_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO6618029.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLCEVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYQYPQQLPPPLPVTYRDYLQQPAIQSLNA
                     DSLAWWQAQLDDIPPAPALPLRCMPQDVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQVYESEGELRFNWDFVAALFPAGQVERQFEQYCTLLN
                     RMTEDENSWHLPLAALVPPVKQAEQGTERTSRVCPEHSQSHIAADESTVSLICDAFRE
                     VVGESVTPAQNFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALAAYLTSV
                     ATVEKTKRSRPVRRRQRRI"
     gene            7397..16888
                     /gene="irp1"
                     /locus_tag="ACHKUC_001764"
     CDS             7397..16888
                     /gene="irp1"
                     /locus_tag="ACHKUC_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFO6618030.1"
                     /translation="MDNLRFSSAPTADSIDASIVQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGAQVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGTRVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASVVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAEP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            16885..17985
                     /gene="ybtU"
                     /locus_tag="ACHKUC_001765"
     CDS             16885..17985
                     /gene="ybtU"
                     /locus_tag="ACHKUC_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO6618031.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETAGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            17982..18785
                     /gene="ybtT"
                     /locus_tag="ACHKUC_001766"
     CDS             17982..18785
                     /gene="ybtT"
                     /locus_tag="ACHKUC_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO6618032.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            18789..20366
                     /gene="ybtE"
                     /locus_tag="ACHKUC_001767"
     CDS             18789..20366
                     /gene="ybtE"
                     /locus_tag="ACHKUC_001767"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO6618033.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            20497..22518
                     /gene="fyuA"
                     /locus_tag="ACHKUC_001768"
     CDS             20497..22518
                     /gene="fyuA"
                     /locus_tag="ACHKUC_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO6618034.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         4560 a         6893 c         6496 g         4625 t
ORIGIN      
        1 cggttctatt tatacccgca ttggtctaag ccacagggag ataaccaggt catgatggag
       61 tcaccgcaaa cgcaatctga aatctctatt caccagttgg tggtcgggaa acctgcaaac
      121 gatggcaata ttcctgctca gtgtgaactg ctgcgctgtt cgttgcagga aggtatggat
      181 attttgctct ggaggggcca ttttgcgcgg cctgagacgt tgcagctaca cgacgatctg
      241 ggcaggatta acttctcgtg catcctggaa ggcacatcgc gctttgcgat tcagggactc
      301 cggcgccaca ccgactggga gcttgcgcgc aatcgccatt acattaccca tacgccagac
      361 tgtcgcggca gcgcctccta ttgtggccga ttcgagagca ttaccctctc cttcagccct
      421 gaaaccctcg cgctgtgggt gccggatatt agcgcggtca ttaagaataa gatcgactcc
      481 cactgctgtt gccagcagca gcgttgtaat gctgaaaccc acttaacggc tcaggccttg
      541 cgtcacgcgc tgatgcgcat gcacggcggc ttcagtcatg aacaaaagcc gtcgacgcta
      601 tggttgcagg ggcaaagtct ggtcatgctc agtctggtgc tggatgaaca tcgcgaagac
      661 gccagttgcc tctcctgcca cttcaacccg atggaacgcc agaaactgct gcgggcgaag
      721 gatctgctgc tggctgatct gacgcaagcg ccgggcgtcg cggagttggc cagagagtcc
      781 ggtctgagcg ttctcaaaat taagcgaggc ttccgcgttc tgttcaacaa cagcgtctat
      841 ggcctgtttc aggctgaacg aatgcaggaa gccagaagac gcctggccaa cggcaatacc
      901 tccgtcatga cggtggcggc cgatctgggc tatgccaacg ccagccattt ctctgccgcc
      961 ttccagaaac agtttggcgt aactccttcg acctttaaac gcgggatgta accggggtcg
     1021 cgccccccta aactcctccc tgacagaggc ccggataccc catccgggcc tctgttcttt
     1081 ccgcctgtta ccaacccgaa acgggcataa ataaacccgt ttcgggtagc atccaattgt
     1141 taataattat tattctcata tgagtaatgc ttttcggtaa gacgtgccat caggaggaag
     1201 aatgatttct ggcgcaccat ctcaggattc gctgttaccg gacaaccgcc acgcggctga
     1261 ttaccaacaa ttacgcgagc ggctcataca ggaactgaat ttaacgccgc agcagttaca
     1321 tgaagagagc aacctgatcc aggccggcct ggattccata agattgatga gatggttaca
     1381 ctggtttcgt aaaaatggct accgccttac ccttcgcgag ctgtatgccg cccccacgct
     1441 ggcggcatgg aaccagttaa tgctcagccg gtcgccggag aacgcggaag aagaaacgcc
     1501 gcccgacgaa tcatcctggc cgaacatgac cgaaagtacc cccttcccat tgacgccagt
     1561 acagcacgcc tacctgacgg gccgcatgcc ggggcagacg cttggcggcg tgggttgcca
     1621 cctgtatcag gagtttgaag gccattgtct gacggcgtcg cagctggagc aggccatcac
     1681 gaccttgctg caacgccacc caatgctgca tatcgccttt cgccccgacg ggcagcaggt
     1741 ctggctaccg caaccttact ggaacggcgt caccgttcat gatttacgcc ataacgacgc
     1801 tgaaagccgc caggcctatc tggacgcact gcgccagcgc ctgagccacc gtcttttacg
     1861 cgtggaaatc ggcgaaacgt ttgattttca gctgacgctc ttgccggaca atcgccaccg
     1921 cctccatgtc aatattgacc tgctgattat ggatgcctcc agctttacgc ttttcttcga
     1981 tgagcttaac gccctgctgg ccggagaatc gctgccggct atcgacaccc gctatgattt
     2041 ccgctcgtat ttgctgcacc aacagaagat caatcaacca ctgagagacg acgcgcgcgc
     2101 ttactggctg gcgaaagcat cgacgcttcc ccccgcgccc gtcttgccgc tggcctgcga
     2161 acccgccacg ctatgtgaag tccgtaatac ccgacgccgc atgattgttc cggcaacacg
     2221 ctggcacgcc tttagcaacc gggccggcga gtatggcgtg acgccgacaa tggcgctggc
     2281 gacctgtttt tctgccgtgc tggctcgctg gggcggcctg acgcgtctgc tgcttaacat
     2341 caccttattc gaccgccagc cgctgcaccc ggcggttggc gcgatgcttg ccgacttcac
     2401 caatattctt ctgctggaca ccgcctgcga tggcgatacc gtcagcaacc tggcgcgtaa
     2461 aaaccagctc acgtttacgg aggactggga gcatcgccac tggtccggcg tcgaattact
     2521 ccgtgaactc aaacgccagc agcgctaccc ccacggcgcc ccggtggtat ttaccagcaa
     2581 tctggggcgt tccctctaca gcagccgcgc agaatcgccg ttgggcgagc cggaatgggg
     2641 catctcgcaa acgccgcagg tctggataga tcatctggcg ttcgagcatc acggcgaggt
     2701 ctggctacaa tgggacagca acgacgcgct gttccctccg gcgttagtcg aaacattgtt
     2761 cgacgcctac tgccagttga ttaaccaact ctgcgatgac gaaagcgcct ggcaaaagcc
     2821 gttcgcagat atgatgcccg ccagccagcg cgcgatacgc gaacgggtca acgccaccgg
     2881 tgcccccatt cccgaaggct tgctgcatga aggcattttc cgtatcgctc tgcaacagcc
     2941 gcaggcgctg gcggtaacgg acatgcgtta tcagtggaat tatcatgagc tgacagacta
     3001 tgcccgccgt tgcgcgggca ggttaatcga gtgcggggtt cagcccggcg ataatgtggc
     3061 tatcacgatg tcgaaaggcg caggacaact tgttgcggtt ctggccgtcc tgctggccgg
     3121 ggcggtttac gttccggttt cgctggatca gcctgccgca cggcgcgaga aaatctacgc
     3181 tgacgccagc gtccggctgg tgctcatttg tcagcacgac gccagcgccg ggtcagacga
     3241 tattcccgcc cttgcctggc agcaggccat tgaggcggag ccgatcgcca acccggtagt
     3301 acgcgccccc acgcaaccgg cctacattat ctacacctcc ggctctaccg gtacgccgaa
     3361 aggggtagtc atttctcacc ggggagcgct taacacctgt tgcgatatca atacccgcta
     3421 tcaggttggc ccgcatgaca gggtgctggc cctctccgcc ctacattttg atttatcggt
     3481 ttacgacatt tttggcgtac tgcgcgcggg cggcgcgctg gtgatggtga tggaaaatca
     3541 acggcgcgat cctcacgcat ggtgtgagct gatccagcgc catcaggtca cgctctggaa
     3601 cagcgtcccg gcgctgttcg atatgctgct gacctggtgt gaaggtttcg ccgacgccac
     3661 gccggaaaac ctgcgcgcag tgatgctttc cggcgactgg atcgggcttg acctccccgc
     3721 ccgttatcgg gccttccggc cacaaggaca atttatcgcg atgggcggcg ccaccgaggc
     3781 gtctatctgg tctaacgcct gcgaaattca cgacgtcccc gcccactggc gctccatccc
     3841 ttacggtttt ccgctaacca accaacgcta ccgggtggtg gatgaacagg gccgggactg
     3901 ccctgactgg gtgccgggtg aattatggat tggcggcatt ggggtcgcgg aaggctattt
     3961 caacgatccc ctgcgtagcg agcagcaatt tttgacgctc ccggacgagc gctggtatcg
     4021 caccggcgat ctcggctgct actggccaga tggcacaatc gagttcctcg gtcgtcgcga
     4081 caagcaggtc aaagtcggag gatatcgcat cgagctgggc gaaatcgaaa gcgcgctcag
     4141 ccagctggcg ggggtgaaac aagcaaccgt tctggcgatc ggcgaaaaag aaaaaacgct
     4201 ggcggcatac gttgttcctc agggcgaggc tttttgcgtt accgatcatc ggaacccggc
     4261 actgccgcag gcgtggcaca cgcttgcggg aacgttgccc tgttgcgcca tctcgccaga
     4321 gatctccgca gaacaggtag ccgatttcct tcagcatcgc ctgctaaaac tgaagccggg
     4381 tcacaccgct ggcgccgatc ctctccccct gatgaactca ctcgctatcc agccgcgctg
     4441 gcaggccgtg gtggaacgct ggttagcatt tctggtgaca caacggcgac tgaagcccgc
     4501 tgctgaaggt tatcaggtct gcgctggtga agaacgcgag gatgagcacc cgcacttcag
     4561 cggacatgat ttaacgttat cgcaaattct tcgcggtgcc cgtaacgaac tgtcgttact
     4621 gaacgacgcg cagtggtccc cggaaagcct ggcctttaac catccggcca gcgccccgta
     4681 tattcaggaa ctggcgacaa tttgccaaca gcttgcacag cgcttacagc gcccggtacg
     4741 cctgcttgag gtgggaaccc gcactggccg cgccgcagaa tcgctgttag cacagctcaa
     4801 cgccggacag attgagtatg tcgggcttga gcagagccag gagatgctgc tgagcgcccg
     4861 gcagaggctc gccccctggc ctggcgcccg tctgtccctc tggaatgcag acacgctggc
     4921 ggcgcacgct cactcggcgg acattatctg gcttaataac gccctgcatc gtctgctgcc
     4981 ggaagatccc gggctccttg cgacattaca acagcttgcc gttcccggcg cgctgctcta
     5041 cgtgatggag tttcgccagt taacgccgtc cgccctactc agcacgctcc tgttaaccaa
     5101 tgggcagccg gaggccttgc tgcataacag cgccgactgg gcggcattat ttagcgcggc
     5161 cggcttcaac tgtcagcatg gcgatgaggt cgcggggtta caacgcttcc tcgtacaatg
     5221 tcctgacagg caggtgcgcc gcgatccccg tcaacttcag gccgccctcg ccgggcgtct
     5281 gccggggtgg atggtgccgc aacggatcgt cttcctcgac gccttaccgc tgacggctaa
     5341 cgggaaaatt gactaccagg cgctgaagcg tcgtcatacc cctgaagcgg aaaacccggc
     5401 cgaagcggat ttaccccagg gcgacattga aaaacaggtt gccgccctct ggcagcaact
     5461 cttatcaact ggcaatgtca ccagagaaac cgacttcttc cagcaaggcg gcgatagcct
     5521 gctggcgacc cgtctgaccg ggcaacttca tcaggcaggt tatgaagcgc aattaagcga
     5581 cctgtttaat catcctcggc tggcggattt tgccgccacg ctgcggaaaa ccgacgtccc
     5641 ggtcgaacaa ccattcgtcc actcccctga agatcgctac cagccctttg cgcttaccga
     5701 cgtgcagcag gcttacctgg tggggcgtca gccgggcttt gccctgggcg gcgtcggctc
     5761 acatttcttt gttgaatttg aaattgccga tctggatctc acccggttgg agacggtctg
     5821 gaaccgatta atcgcccgcc acgatatgct acgcgccgtc gtgcgtgatg gacagcaaca
     5881 ggtgctcgaa cagacgcctc gctgggtaat acccgcacac atcctccata cgcctgaaga
     5941 ggcgttgcag gtgcgcgaaa aactggccca tcaggtactc aaccccgaag tctggccggt
     6001 attcgatctc caggtcggtt acgttgacgg gatgcccgcc cgcctgtggc tgtgtctgga
     6061 taacctgtta cttgacgggc tgagcatgca gattctgttg gcggagctgg aacatggcta
     6121 ccaatatccg caacagttgc ctccgccgct acccgtcacc tacagggatt acctgcaaca
     6181 acccgcgatc cagtcgctta acgcagattc tctggcatgg tggcaggcgc aacttgatga
     6241 tattcctccg gctcctgcat tgccgctacg ttgcatgcct caggacgtcg aaacaccgcg
     6301 cttcgcccgc ctgaacggcg cgctggacag cacgcgctgg catcggctga aaaaacgggc
     6361 ggctgacgcc catctcaccc cgtcggccgt actgttgtcg gtgtggtcaa cggttctctc
     6421 tgcatggagt gcacagcctg agttcacgct taaccttacg cttttcgaca ggcgaccgct
     6481 gcacccgcaa atcaaccaga ttctgggcga tttcacctcg ctgatgctgc tgagctggca
     6541 tcccggcgaa agctggctgc acagcgcgca gtcactacag cagcggctga gccagaacct
     6601 caaccaccgc gatgtgtcag ccatccgcgt gatgcgtcaa ctggcgcaac ggcaaaacgt
     6661 gcctgccgtt ccgatgcccg tcgtctttac cagcgcgctg ggctttgagc aggataactt
     6721 cctcgcccgg cgtaatctgc tcaaaccggt ctggggcatc tcccagacgc cgcaggtctg
     6781 gctcgatcac caggtttatg aatccgaagg cgaactgcgc tttaactggg attttgtcgc
     6841 cgcgctgttt cctgccgggc aggtggaacg tcagtttgag cagtattgca ccttgctaaa
     6901 ccgaatgacc gaggatgaaa acagctggca tttgccactc gccgcgctgg tgcctcccgt
     6961 taagcaggcg gagcaaggta cagagcgcac atcgcgcgta tgccctgagc actctcagtc
     7021 acacattgcg gcggacgaga gcaccgtcag cctgatttgc gacgccttcc gcgaggtggt
     7081 tggcgagtct gtcacgcccg cacaaaactt ctttgaggcg ggcgcaacgt cgctgaatct
     7141 ggtgcaactg cacgttttgt tacaacgtca cgaattttcg actctgacgt tacttgacct
     7201 ctttacccac ccttctcctg cagccctggc cgcttatctg accagtgtcg ccacggtgga
     7261 gaaaaccaaa cgctctcgcc ctgttcgccg tcgtcagcgg cggatatagc gcgaagcaaa
     7321 ctgattttcc ccggaacgcc atcgcgaacg catggcgttc cattgacttt atgaacctta
     7381 ggaaatggga ccgattatgg ataacttgcg cttctcttct gcgccaacag cagattccat
     7441 tgatgcatcg atcgttcaac actacccgga ctgcgaacct gtcgcggtta tcggctacgc
     7501 ctgccatttt cctgaatcgc cggatggcga aacgttctgg caaaatctgc tggaaggtcg
     7561 tgaatgcagc cgacgcttta cgcgcgaaga gcttctggcc gtcggtctgg atgccgccat
     7621 cattgacgat cctcattatg tcaatatcgg tacggtgtta gacaacgccg actgcttcga
     7681 cgccaccctg tttggctatt cgcgacagga agcggagtcg atggacccgc agcagcgcct
     7741 gtttttgcag gcggtctggc atgcgctgga acatgccggt tatgcccccg gcgccgtccc
     7801 ccataagacc ggcgttttcg cctcttcccg gatgagtacc taccccggtc gcgaagcatt
     7861 gaacgtgaca gaagtcgcgc aggtaaaagg tctgcaatct ctgatgggca atgataaaga
     7921 ctatattgcc acccgcgccg cgtacaaact caacctgcac ggcccggcgt tatcggtaca
     7981 gaccgcctgc tccagctcgc tggttgccgt gcatctggcc tgtgaaagcc tgcgcgctgg
     8041 cgaatccgat atggcggttg ccggcggcgt ggcgctctct ttcccccagc aggcaggcta
     8101 ccgctaccag cccggaatga ttttctctcc tgatggtcac tgtcgtccct ttgacgcctc
     8161 ggctgagggc acctgggccg gtaacggtct cggctgcgtg gtgctgcgtc gcctgagaga
     8221 cgcgctgctg tcaggcgatc cgattatctc ggtgatcctc tccagcgcgg tcaacaacga
     8281 cggcaacaga aaggtcggct ataccgcccc ttccgtcgca gggcaacagg cagtcatcga
     8341 agaggcgtta atgctggcgg ccatcgacga caggcaggta ggttacattg aaacccacgg
     8401 caccggcaca ccgctgggcg acgcgattga aattgaagcg ttacgcaacg tctatgcgcc
     8461 tcgcccgcag gatcagcgct gtgcgctcgg ttccgtgaaa agtaacatgg gccatctgga
     8521 taccgcggcg ggcattgccg gactgctgaa aaccgttctg gcagtcagtc gcgggcaaat
     8581 tcctccctta ctgaattttc acacccccaa cccggcgctg aaacttgaag agagcccctt
     8641 taccataccg gtgtcggcac aggcatggca ggacgaaatg cgctatgcgg gcgtctcctc
     8701 ctttggtatt ggcggcacca actgccatat gatcgtcgcc tcgctgcccg acgcgctcaa
     8761 cgcgcgcctc cccaatacgg atagcggcag aaaaagtacc gcgctgctgc tcagcgccgc
     8821 cagcgacagc gcgttgcggc ggctggcgac ggattatgcc ggggcgctga gagagaatgc
     8881 ggatgccagc tctctggcct tcacagccct gcacgcgcgc cgtctcgatc tccccttccg
     8941 cctggcggcg ccattaaacc gtgaaaccgc cgaggcgctc agcgcctggg ccggtgagaa
     9001 atcgggggcg caggtttaca gcggccacgg cgccagcggc aagcaggtgt ggctgtttac
     9061 cggccagggc tcgcactggc gcactatggg tcaaacgatg taccagcact caacggcgtt
     9121 tgccgacacg ctggatcgct gtttttccgc ctgtagcgaa atgctcacgc cgtcactgcg
     9181 cgaagcgatg tttaaccccg attcggcgca gctggacaat atggcctggg cgcagccggc
     9241 gattgtcgcg tttgaaatcg cgatggcggc gcactggcgt gctgaaggac tgaagccaga
     9301 cttcgccatt gggcattccg tcggtgaatt tgccgctgcc gttgtctgcg gacactatac
     9361 gattgaacag gtcatgccac tggtttgtcg gcgcggcgcg ctaatgcagc agtgcgcaag
     9421 cggcgcgatg gtggcggtat ttgcagacga agacacgctg atgccgctgg ctcgccagtt
     9481 tgagctggat ctcgccgcca acaacggtac gcaacatacg gtattttccg ggccggaagc
     9541 ccgtctcgcg gtattttgcg ccacgctctc gcagcatgac attaactatc gtcgcctgag
     9601 cgtaaccggt gcggcgcact ccgctttact ggagccgata ctcgatcggt tccaggacgc
     9661 ctgcgcggga ctgcacgcgg agccggggca aataccgatt atttccacgc tcaccgccga
     9721 cgtcattgat gagtcaacgc tcaaccaggc ggattactgg cgccgacaca tgcgccagcc
     9781 ggtgcgtttt atccagagta ttcaggtggc gcatcaactc ggcacccgcg tttttctgga
     9841 gatggggccc gatgcccagt tggttgcttg cgggcagcgc gaataccgcg ataacgcata
     9901 ctggatagcc agcgcccggc gtaacaaaga ggcgagcgtt gtcctcaatc aggccctgct
     9961 ccagctttac gctgccggcg tcgctctacc gtgggccgac ctactggcgg gcgatggaca
    10021 acgtatcgct gcgccatgtt atccgtttga tactgagcgt tactggaaag agcgcgtctc
    10081 cccggcctgc gagcctgccg acgcagcgct gtctgccggg ctggaggtgg cgagtcgcgc
    10141 cgcgacagcg ctcgatctcc ctcgcctgga agcgcttaaa cagtgcgcca cgcgactgca
    10201 cgccatctac gtcgatcaac tggtacaacg ctgtaccggc gatgccattg agaacggcgt
    10261 ggacgccatg accatcatgc gccgtggacg tctgctgccc cgctaccagc agctactcca
    10321 gcgcctgctg aataactgcg tggtcgacgg cgattaccgc tgcaccgacg ggcgatacgt
    10381 ccgcgcccgc cccattgaac atcaacagcg ggaatcactg ctgacggaac ttgccggtta
    10441 ttgtgaaggt tttcaggcta ttcccgacac catcgcccgt gccggcgatc ggttatatga
    10501 aatgatgagc ggcgcggaag aaccggtggc gattatcttc ccgcaaagcg cctccgacgg
    10561 cgtggaagtg ctgtatcagg aattcagctt tggccgctat ttcaaccaaa tcgccgccgg
    10621 ggtattacgt ggcattgtcc agacgcgtca gccccgccag ccgttgcgta ttcttgaagt
    10681 tggcggcgga accggcggca ccaccgcgtg gctgctgccg gaactcaacg gcgttccggc
    10741 actggagtac catttcaccg atatctcggc gctgttcacc cgtcgcgccc agcagaaatt
    10801 cgccgactat gattttgtga agtatagcga gctggatctc gaaaaagagg cgcagtctca
    10861 gggtttccag gcacagtctt acgatcttat cgtggcagcg aacgtgattc acgccacccg
    10921 ccatattggc cgcacgctcg ataatctgcg ccccctgctc aagccgggcg ggcgcctgct
    10981 gatgcgcgaa atcacccagc caatgcgtct gtttgacttc gttttcggcc cgctggttct
    11041 tccgctacag gatctcgacg cccgcgaagg tgagttattc ctcaccaccg ctcagtggca
    11101 acaacagtgc cgccacgccg gattcagcaa agtggcgtgg ctaccgcagg atggcagccc
    11161 gaccgccggg atgagcgaac atatcattct cgccacgctg cccggtcagg cggttagcgc
    11221 cgtaacattc accgcgccat cagaacccgt gttggggcag gcgctgacgg ataacggtga
    11281 ttatctcgcc gactggtctg attgcgcagg tcagcccgaa cggtttaacg cccgctggca
    11341 ggaggcctgg cgtctgcttt cacagcgtca tggcgacgct ctgcctgtgg aaccgccccc
    11401 cgtcgccgcc ccggagtggc tagggaaggt tcgcttaagc tggcaaaacg aagccttttc
    11461 ccgcggtcag atgcgcgttg aagcccgtca tcctgctggc gagtggctgc cgctatcgcc
    11521 cgccgagcct cttcctgcgc cgcaaacgca ttatcaatgg cgctggacgc ccctcaacgt
    11581 cgccagcatt gaccatccgc ttacctttag cttcagcgcc ggtacgcttg cgcgcagcga
    11641 cgagctggcg caatacggca tcattcacga tccgcacgcc tcttcacgac tgatgattgt
    11701 tgaggagagc gaggatacgc tggccttagc ggagaaagtg atagcagcgc tcaccgccag
    11761 cgcagccgga ttgattgtgg ttactcgccg cgcgtggcga gtcgaggaaa atgaagcact
    11821 ctctgcatcc catcacgcgc tatgggcctt gcttcgcgtc gcggccaacg aacagccgga
    11881 acggttgctt gccgccatcg atctcgccga aaacaccccg tgggaaacgc tgcatcaagg
    11941 gttgagcgca gtctcactat cacagcgctg gctcgccgca cggggtgaca ccctttggct
    12001 tccttcactg tcgcccaata cgggatgcgc cgctgaatta ccggcaaacg tgtttaccgg
    12061 cgatagccgc tggcatctgg tgaccggagc gtttggcgga ttaggccgcc ttgccgtgaa
    12121 ctggctcaga gaaaaagggg cgcgacgcat cgccctgcta gcgccgcgcg tggatgagtc
    12181 atggctacgc gacgtggagg gcgggcagac gcgcgtctgc cgttgtgatg tgggcgatgc
    12241 cgggcaactg gccacggttc ttgacgatct ggcggccaac ggcggcattg ccggagcgat
    12301 tcatgccgct ggcgtattgg ctgacgcgcc cttgcaggag cttgatgacc accagctggc
    12361 tgccgttttc gcggtaaaag cgcaggcggc aagccagctg ttgcaaaccc tgcgcaacca
    12421 cgacggacgc tatcttattc tctactcttc cgctgccgcc accctcggcg cgccgggtca
    12481 gagcgcccat gcgctggcct gcggctacct ggacgggctg gcccagcagt tttccaccct
    12541 tgatgcgccg aaaacgctct ctgtcgcctg gggcgcatgg ggagaaagcg gtcgggcggc
    12601 cacgccggaa atgctggcga cgctcgccag ccgaggtatg ggcgcgttaa gcgatgccga
    12661 aggctgctgg cacctggaac aggcggtgat gcgcggtgcc ccgtggcgac tggcgatgcg
    12721 cgtttttacc gacaaaatgc ccccgttaca acaggctctg tttaacatca gcgccacaga
    12781 aaaagccgca acgccggtca ttcctcctgc tgatgacaac gcctttaacg gcagcctgag
    12841 cgatgaaaca gcggtgatgg catggctgaa aaagcggatt gcggttcagc taaggctgag
    12901 cgatccggcg tcactgcatc caaaccagga tctgttgcaa ctcggcatgg actcgctgct
    12961 cttccttgaa ctcagtagcg atattcagca ctacctgggc gtacgcatca atgcggaacg
    13021 ggcgtggcag gatctgtctc ctcatggact cacgcagctt atctgttcta agccagaggc
    13081 gacgcctgcc gcttcgcagc cggaagtgtt gcggcacgac gccgacgagc gttatgcgcc
    13141 cttccctttg acgcccattc agcacgccta ctggctgggg cgaacccacc tcattggcta
    13201 tggcggcgtc gcctgtcacg tcctgtttga gtgggataaa cgccacgatg agttcgatct
    13261 cgccatactg gagaaagcat ggaaccagct catcgcacgc cacgatatgt tgcgtatggt
    13321 ggttgatgcc gacgggcagc agcgaatcct ggcgacaacg ccggagtatc acatcccgcg
    13381 tgacgatctg cgcgcgcttt ccccggaaga acagcgcatc gcgctggaaa aacggcggca
    13441 tgaactgagc tatcgcgttt tgcctgccga ccagtggcct ctttttgagc tggtggtcag
    13501 cgaaatcgac gattgccatt accgtctgca tatgaacctc gaccttttgc agtttgatgt
    13561 gcagagtttt aaagtcatga tggacgacct ggcgcaggtc tggcgcggtg aaacgctggc
    13621 gccgctcgct attaccttcc gtgattatgt gatggctgaa caggcgcgcc gacagacatc
    13681 ggcatggcac gatgcctggg attactggca ggaaaaactg ccgcaactgc ccttagcgcc
    13741 agagctgccg gtggttgaga cgcccccgga aacgccacac ttcaccacct tcaaatcgac
    13801 gatcggcaag acagaatggc aggccgtgaa acagcgctgg cagcagcaag gcgtcacacc
    13861 gtctgccgcg ctgctcacgc tgtttgccgc cacccttgag cgctggagcc gtaccacaac
    13921 atttacgctg aacctgacgt tcttcaatcg ccagccgatc catccgcaaa tcaaccagtt
    13981 gattggtgat tttacctccg tcacgctggt tgattttaac ttctcagcgc cggtgacgtt
    14041 gcaagagcag atgcaacaga cccaacagcg cctctggcaa aacatggcgc acagtgaaat
    14101 gaacggtgtt gaggtgatcc gtgagctggg ccgcctgcgc ggatcacaac gtcaaccgct
    14161 gatgccggta gtgtttacca gtatgctggg gatgacgctg gaaggcatga ctatcgatca
    14221 ggcgatgagc catctgttcg gcgaaccctg ctatgtattc acgcaaacgc cgcaggtctg
    14281 gctggatcat caggtcatgg agagcgacgg cgagttgatg tttagctggt actgcatgga
    14341 caacgtgctg gaacccggcg ctgccgaggc gatgtttaat gactattgcg ccatcctgca
    14401 agccgtcatc gccgcccctg aaagcctgaa gactctcgcc agcggcatcg ccgggcacat
    14461 tccccgccga cgctggccgc tgaacgcgca ggcggactac gacctgcggg atattgagca
    14521 ggcgacgctc gaataccccg gcatccggca ggccagagcg gaaataaccg aacagggcgc
    14581 gttgacgctg gatatcgtga tggccgacga tccgtcgcca tcagcggcga tgcctgatga
    14641 gcacgaactt acccaactgg cgctgccgtt gcctgagcag gcgcagcttg atgagctgga
    14701 ggcgacctgg cgctggctgg aggcgcgtgc gctacagggg atcgcggcta cgctaaatcg
    14761 tcacggcctg tttaccacgc cggagatcgc ccatcgcttt agcgcaatag tacaggcgct
    14821 gtccgcgcaa gcgtctcacc agcgtctgct gcgccagtgg ctacagtgtc tgacggaaag
    14881 agagtggtta atccgcgaag gtgaaagctg gcgctgccgc attccgctca gcgagattcc
    14941 tgagcctcag gaagcgtgcc cgcaaagcca atggagccag gcgctggcgc agtatctgga
    15001 aacctgcatc gcccggcacg acgccctctt ctccgggcag tgttctccgc tggaattgct
    15061 gttcaacgag cagcatcgcg ttaccgacgc gctgtatcgc gacaaccccg ccagcgcctg
    15121 tctgaatcgc tataccgcgc agattgccgc cttgtgcagc gcagaacgga ttctggaggt
    15181 tggcgccgga accgcagcca ctaccgcgcc ggtgctgaag gccacgcgga acacgcggca
    15241 gtcgtaccac ttcacggacg tctccgcgca gttcctcaat gacgccagag cccgtttcca
    15301 tgatgaatcg caggtgtctt atgccttgtt cgacatcaac cagccgctgg atttcaccgc
    15361 ccacccggag gcgggttacg acctgatcgt tgccgtcaat gtgctccacg acgccagcca
    15421 tgtcgtccag acgttgcgca gattaaaact gttgctgaaa gccggcggac gtttgctgat
    15481 cgttgaagcg acggagcgaa acagcgtatt ccagctggcg agcgtgggct ttattgaggg
    15541 attaagcgga taccgcgatt tccgccgccg ggatgagaaa ccgatgctca cccgctccgc
    15601 atggcaggag gttctcgttc aggccgggtt tgcaaacgag ctggcgtggc ccgcgcagga
    15661 atcgtcgccg ctgcgccagc atctgctggt agcgcgttcg cctggcgtaa atcgcccgga
    15721 taaaaaagcc gtgagccgct atttacagca gcgctttggc accggtctgc ccattttaca
    15781 gatccggcaa agagaagcgt tatttacgcc gctgcatgcc ccgtctgatg cgccgactga
    15841 gccagccaaa cccacgccag ttgccggggg gaatccggcg ctggaaaaac aggtggctga
    15901 actctggcaa tcgctgctgt ctcgccccgt ggcaaggcat cacgactttt tcgaactggg
    15961 cggcgacagc ctgatggcga caaggatggt cgcgcagcta aaccggagag ggattgccag
    16021 ggctaacctt caggatctgt tcagccattc gacgctgagc gacttctgcg cccatctaca
    16081 ggcggctacg tcaggagagg acaacccgat acccctttgc cagggcgacg gtgaggaaac
    16141 cctgtttgtc ttccacgctt cagacggcga tatcagcgcc tggctgccgc tcgctagcgc
    16201 gttgaacagg cgcgttttcg gcctgcaagc aaaatcgccg cagcgctttg ccacgctcga
    16261 ccagatgatc gatgagtatg tcgggtgcat ccgtcgtcag cagcctcacg gcccttatgt
    16321 gctggcgggt tggtcgtatg gcgcgtttct tgcggcgggc gccgcacagc gcctgtacgc
    16381 caaaggcgag caggttcgga tggtgttaat cgatcccgtg tgccgacagg atttctgttg
    16441 cgaaaaccgg gcggccctgc tgcgcctgtt agccgaagga caaacgcctc tggcactgcc
    16501 cgaacatttc gaccagcaga cgcccgacag ccagcttgcc gactttatca gcctcgctaa
    16561 aacggccggt atggtgtcgc aaaacctgac gctgcaagcg gcagaaacgt ggctcgacaa
    16621 catcgcgcat ctgctgcgtt tactgactga gcatacgccg ggcgaaagcg ttccggtccc
    16681 ctgtctcatg gtgtatgccg ccgggagacc cgcgcgctgg acgccagcag aaaccgagtg
    16741 gcagggctgg ataaacaacg ccgacgacgc tgtgattgaa gccagccact ggcaaatcat
    16801 gatggaagcc ccccacgttc aggcttgtgc gcaacacatt acgcgctggc tttgcgcaac
    16861 ctcaacgcaa ccggagaaca cgttatgatg ccgtccgcct ccccaaaaca acgcgtactg
    16921 attgtgggcg ccaaatttgg cgaaatgtac ctgaatgcct ttatgcagcc cccggagggg
    16981 ctggaactgg tcggcctgct ggcgcaggga agcgcccgtt caagagagct ggctcatgcg
    17041 tttggcattc cgctgtatac ctcgccggaa cagataacca ggatgccgga tatcgcctgt
    17101 attgtcgtgc gttcgaccgt cgccggagga accggtacgc agcttgccag acactttctg
    17161 acgcgcggcg tacacgtcat tcaggagcat cccctccacc cggatgacat cagttcctta
    17221 caaacgctgg cgcaggaaca gggctgctgc tattgggtaa acacgtttta tccgcatacg
    17281 cgcgcgggac gcacatggct tcgcgatgcg cagcagctgc gtcgctgcct ggctaagacg
    17341 ccgccggtag tccacgccac caccagccga cagttgctct actccactct ggatttgttg
    17401 ttgctggctc tgggcgttga tgccgccgcc gtggagtgcg acgtggttgg cagctttagc
    17461 gattttcact gtctgagact cttctggccg gagggagaag cctgcctgct gcttcagcgc
    17521 tatctcgatc ctgacgatcc agatatgcat agccttatca tgcaccgcct gctgctgggc
    17581 tggccggaag gtcatctttc gctggaggcg agctacggcc ccgtcatctg gtcatccagc
    17641 ctgtttgtcg cagaccatca ggagaacgcc cacagcctct accgcagacc ggagatcctg
    17701 cgcgatctgc cgggtctgac gcgcagcgcg gcgcccctga gctggcgcga ctgctgcgaa
    17761 accgccgggc cggagggcgt cagctggttg ctacaccagt tgcgcagcca tctggcgggc
    17821 gaacatccac cagcggcctg ccaaagcgta catcagatcg cgttatcacg tttatggcag
    17881 cagattttac gtaagaccgg caatgcggag atcagacgcc tgacgccgcc gcaccacgac
    17941 cggttagccg gtttctacaa cgatgatgat aaggaggcgc tgtgacgcaa tctgcaatgt
    18001 gcatcccgct gtggcccgcc cggaacggca atactgcgca tctggtcatg tgccctttcg
    18061 ctggcggcag cagtagcgcg tttcgccact ggcaagctga gcaactggct gattgcgcgc
    18121 tttctctggt gacctggccg gggcgcgatc gccttcgcca tctggaaccg ctcagaagca
    18181 ttacacaact ggcggcactg ctggcgaacg agctggaagc atccgtatcg cctgacacgc
    18241 cgcttttact cgccgggcac agcatggggg cgcaggtggc gtttgaaacc tgccgacttc
    18301 tggagcaacg ggggcttgcg ccacaaggac tgattatttc cgggtgccat gccccgcatc
    18361 tgcattctga acgccagctc agccatcgcg atgatgccga ctttatcgct gagctgatag
    18421 acattggcgg atgttctcct gaactgcggg aaaaccagga attaatgtcg ctgtttcttc
    18481 ctcttctgcg cgctgatttt tacgccaccg agagctatca ctacgactcg cccgacgtct
    18541 gtccgccgct gcgcacgcct gcgctgttat tgtgcggcag ccacgatcgc gaagcctcct
    18601 ggcagcaggt cgatgcctgg cgtcagtggc tgagccacgt tacaggcccg gtggtgattg
    18661 acggcgatca tttctatccc attcaacaag cccggtcctt ttttacgcag attgtccgcc
    18721 attttcccca cgcattttct gcaatgaccg cgtggcaaaa acagcccagt acttcagaaa
    18781 ggtgacgcat gaattcttcc tttgaatctc tgattgaaca gtatccctta cccattgccg
    18841 aacagttgcg ccactgggcg gcccgttatg cctcgcgaat tgccgtcgtt gatgcaaagg
    18901 ggtcgttaac ctacagcgcg cttgatgcac aagttgacga acttgccgca ggtctgtcat
    18961 cactgggttt gcgttcgggg gagcatgtaa ttgtgcagct tcccaacgac aacgcgtttg
    19021 ttaccctgct gttcgccttg ttaagactgg gcgttatccc cgtgctggcg atgccctcgc
    19081 aacgggcgct ggatatcgac gcgctgattg agctggcgca acccgtcgct tacgttattc
    19141 acggggaaaa ccacgcagag ctggcccgac agatggcgca caaacacgcc tgcttgcgtc
    19201 atgttctggt cgctggagag accgtgagcg acgattttac gccgctcttc tcccttcacg
    19261 gtgagcgaca ggcatggccg cagcctgatg tttccgccac cgcgttgttg ttgctctcag
    19321 gcggcacaac cggcacgccc aaactcatcc cgcgccgaca tgccgactat agctataact
    19381 tcagcgcttc tgctgaactg tgcggcatca gccaacagag cgtgtatctc gccgtcctcc
    19441 cggtggcgca taactttccg ctggcctgcc ccggtattct gggaacgctt gcctgcggcg
    19501 gaaaagtggt gctgaccgac agcgccagct gtgatgaggt gatgccttta atcgcgcagg
    19561 aaagagtgac tcacgtcgcc ctggttccgg cgctggcgca attatgggtg caggccaggg
    19621 agtgggaaga cagcgacctt tcgtcgctgc gcgtcattca ggcaggcggc gcccggctcg
    19681 acccgacgct tgctgagcag gttatcgcca cctttgactg taccctgcaa caggttttcg
    19741 gtatggcgga aggcctgctc tgttttaccc ggctggacga tccgcatgcc accattctcc
    19801 acagccaggg gcgcccgttg tcccctctgg atgaaatccg catcgttgat caagacgaga
    19861 acgacgtcgc gccgggcgaa accgggcaat tgttaacgcg cggcccttat accatttcgg
    19921 gctattaccg cgcccctgcc cacaatgcgc aggcctttac cgcgcaaggg ttttaccgca
    19981 caggcgacaa tgtcaggctg gatgaggtgg ggaacctgca cgttgaggga cgcataaaag
    20041 agcagatcaa ccgcgccgga gaaaaaatag ccgcggctga agtggaatcg gcactgctgc
    20101 gtttagcgga agtgcaagat tgcgcggtgg tcgccgcgcc ggacacgctg cttggcgagc
    20161 ggatttgcgc gtttatcatc gcgcagcagg tgccaactga ctatcagcag ttgcgtcaac
    20221 aactgacccg tatggggctc agcgcgtgga aaattcctga ccaaatcgag tttctggacc
    20281 actggccgct caccgccgtc ggcaagatag acaaaaaacg cctgacggct ctcgccgtcg
    20341 accgttatcg ccattctgcc caataagcgc aaaccgaccc gaaacaggtt gaaataaacc
    20401 cgtttcgggt agcaccacta ttagaaatag ttatcatttt caattcacca ttgtcggtat
    20461 ttttggcgtt tcgccgtctt acagggactc acaacaatga aaatgacacg gctttatcct
    20521 ctggccttgg ggggattatt actccccgcc attgctaatg cccagacttc acagcaagac
    20581 gaaagcacgc tggtggttac cgccagtaaa caatcttccc gctcggcatc agccaacaac
    20641 gtctcatcta ctgttgtcag cgcgccggaa ttaagcgacg ccggcgtcac cgccagcgac
    20701 aaactcccca gagtcttgcc cgggctcaat attgaaaata gcggcaacat gcttttttcg
    20761 acgatctcgc tacgcggcgt ctcttcagcg caggacttct ataaccccgc cgtcaccctg
    20821 tatgtcgatg gcgtccctca gctttccacc aacaccatcc aggcgcttac cgatgtgcaa
    20881 agcgtggagt tgctgcgagg cccacaggga acgttatatg gcaaaagcgc tcagggcggg
    20941 atcatcaaca tcgtcaccca gcagccggac agcacgccgc gcggctatat tgaaggcggc
    21001 gtcagtagcc gcgacagtta tcgaagtaag ttcaacctga gcggccccat tcaggatggc
    21061 ctgctgtacg gcagcgtcac cctgttacgc caggttgatg acggcgacat gattaacccc
    21121 gcgacgggaa gcgatgactt aggcggcacc cgcgccagca tagggaatgt gaaactgcgt
    21181 ctggcgccag acgatcagcc ctgggaaatg ggctttgccg cctcacgcga atgtacccgc
    21241 gccacccagg atgcctatgt gggatggaat gatattaagg gccgtaagct gtcgatcagc
    21301 gatggttcac cagacccgta catgcggcgc tgcactgaca gccagaccct gagtgggaaa
    21361 tacaccaccg atgactgggt tttcaacctg atcagcgcct ggcagcagca gcattattcg
    21421 cgcaccttcc cttccggttc gttaatcgtc aatatgcctc agcgctggaa tcaggatgtg
    21481 caggagctgc gcgccgcaac cctgggcgat gcgcgtaccg ttgatatggt gtttgggctg
    21541 taccggcaga acacccgcga gaagttaaat tcagcctacg acatgccgac aatgccttat
    21601 ttaagcagta ccggctatac caccgctgaa acgctggccg catacagtga cctgacctgg
    21661 catttaaccg atcgttttga tatcggcggc ggcgtgcgct tctcgcatga taaatccagt
    21721 acacaatatc acggcagcat gctcggcaac ccgtttggcg accagggtaa gagcaatgac
    21781 gatcaggtgc tcgggcagct atccgcaggc tatatgctga ccgatgactg gagagtgtat
    21841 acccgtgtag cccagggata taaaccttcc gggtacaaca tcgtgcctac tgcgggtctt
    21901 gatgccaaac cgttcgtcgc cgagaaatcc atcaactatg aacttggcac ccgctacgaa
    21961 accgctgacg tcacgctgca agccgcgacg ttttataccc acaccaaaga catgcagctt
    22021 tactctggcc cggtcgggat gcagacatta agcaatgcgg gtaaagccga cgccaccggc
    22081 gttgagcttg aagcgaagtg gcggtttgcg ccaggctggt catgggatat caatggcaac
    22141 gtgatccgtt ccgaattcac caatgacagt gagttgtatc acggtaaccg ggtgccgttc
    22201 gtaccacgtt atggcgcggg aagcagcgtg aacggcgtga ttgatacgcg ctatggcgca
    22261 ctgatgcccc gactggcggt taatctggtc gggccgcatt atttcgatgg cgacaaccag
    22321 ttgcggcaag gcacctatgc caccctggac agcagcctgg gctggcaggc gactgaacgg
    22381 atgaacattt ccgtctatgt cgataacctg ttcgaccgtc gttaccgtac ctatggctac
    22441 atgaacggca gcagcgccgt cgcgcaggtc aatatgggtc gcaccgtcgg tatcaatacg
    22501 cgaattgatt tcttctgatt attgtaaaag ggataccgaa aaggtatccc ttttacacca
    22561 ctagttaaaa ccag
//
LOCUS       DAZFRG010000059        22287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFRG010000059 DAZFRG010000000
VERSION     DAZFRG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.059"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(147..1058)
                     /gene="gsiD"
                     /locus_tag="ACHKUC_001769"
     CDS             complement(147..1058)
                     /gene="gsiD"
                     /locus_tag="ACHKUC_001769"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO6618035.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1061..1981)
                     /gene="gsiC"
                     /locus_tag="ACHKUC_001770"
                     /pseudo
     CDS             complement(1061..1981)
                     /gene="gsiC"
                     /locus_tag="ACHKUC_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
     gene            complement(1999..3537)
                     /gene="gsiB"
                     /locus_tag="ACHKUC_001771"
     CDS             complement(1999..3537)
                     /gene="gsiB"
                     /locus_tag="ACHKUC_001771"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO6618036.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFVGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKKAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3557..5428)
                     /gene="gsiA"
                     /locus_tag="ACHKUC_001772"
     CDS             complement(3557..5428)
                     /gene="gsiA"
                     /locus_tag="ACHKUC_001772"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO6618037.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFDNPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5415..6380)
                     /gene="iaaA"
                     /locus_tag="ACHKUC_001773"
     CDS             complement(5415..6380)
                     /gene="iaaA"
                     /locus_tag="ACHKUC_001773"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6618038.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYRERGDTVATQ"
     gene            6584..7819
                     /gene="moeA"
                     /locus_tag="ACHKUC_001774"
     CDS             6584..7819
                     /gene="moeA"
                     /locus_tag="ACHKUC_001774"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO6618039.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALHAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7819..8568
                     /gene="moeB"
                     /locus_tag="ACHKUC_001775"
     CDS             7819..8568
                     /gene="moeB"
                     /locus_tag="ACHKUC_001775"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO6618040.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8644..9306)
                     /gene="fsa"
                     /locus_tag="ACHKUC_001776"
     CDS             complement(8644..9306)
                     /gene="fsa"
                     /locus_tag="ACHKUC_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6618041.1"
                     /translation="MELYLDTSDVVAVTALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9437..10336
                     /gene="ybiY"
                     /locus_tag="ACHKUC_001777"
     CDS             9437..10336
                     /gene="ybiY"
                     /locus_tag="ACHKUC_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO6618042.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCKLCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10342..12774
                     /gene="ybiW"
                     /locus_tag="ACHKUC_001778"
     CDS             10342..12774
                     /gene="ybiW"
                     /locus_tag="ACHKUC_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO6618043.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSHINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRCDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPEQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12920..13735
                     /gene="ybiV"
                     /locus_tag="ACHKUC_001779"
     CDS             12920..13735
                     /gene="ybiV"
                     /locus_tag="ACHKUC_001779"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO6618044.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(13935..15527)
                     /gene="ybiT"
                     /locus_tag="ACHKUC_001780"
     CDS             complement(13935..15527)
                     /gene="ybiT"
                     /locus_tag="ACHKUC_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO6618045.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            15746..16666
                     /gene="ldtB"
                     /locus_tag="ACHKUC_001781"
     CDS             15746..16666
                     /gene="ldtB"
                     /locus_tag="ACHKUC_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6618046.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16724..17842)
                     /gene="ybiR"
                     /locus_tag="ACHKUC_001782"
     CDS             complement(16724..17842)
                     /gene="ybiR"
                     /locus_tag="ACHKUC_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO6618047.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(17839..18306)
                     /gene="mntR"
                     /locus_tag="ACHKUC_001783"
     CDS             complement(17839..18306)
                     /gene="mntR"
                     /locus_tag="ACHKUC_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO6618048.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18492..18620
                     /gene="mntS"
                     /locus_tag="ACHKUC_001784"
     CDS             18492..18620
                     /gene="mntS"
                     /locus_tag="ACHKUC_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO6618049.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            18892..20475
                     /gene="opgE"
                     /locus_tag="ACHKUC_001785"
     CDS             18892..20475
                     /gene="opgE"
                     /locus_tag="ACHKUC_001785"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6618050.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDNFDPKP"
     gene            complement(20524..21039)
                     /gene="ompX"
                     /locus_tag="ACHKUC_001786"
     CDS             complement(20524..21039)
                     /gene="ompX"
                     /locus_tag="ACHKUC_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO6618051.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21092..21157)
                     /locus_tag="ACHKUC_001787"
     CDS             complement(21092..21157)
                     /locus_tag="ACHKUC_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618052.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            21392..22279
                     /gene="rhtA"
                     /locus_tag="ACHKUC_001788"
     CDS             21392..22279
                     /gene="rhtA"
                     /locus_tag="ACHKUC_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO6618053.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5460 a         6101 c         5503 g         5223 t
ORIGIN      
        1 acgctgtgcc gctgcaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
       61 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
      121 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
      181 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
      241 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgcaccccac
      301 tctggtgtcg gcggctgcgc accgaggccg agaaatgaca ggctggcggc agagataatc
      361 gaggcgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaagata
      421 tgacgcaaca aaatggtcat atcgctggca ccaatactgc gtgctgactc gataaaggtt
      481 tgctgtttca acaccagcgt gttgccgcgc accagccggg caaacgcggg gatggaaaaa
      541 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
      601 gccagtaaaa tacccgggaa ggcaaacaac acatcgcaaa tgcgcatgat cagccgatcc
      661 caccagcctt cataatatcc agccagcaag cccagcaacg tcccgatcgc cgcaccgata
      721 aacacggcaa acacgcccgc cgccagcgat atttgcgcac caaccaggac acggctgaaa
      781 atgtcgcgcc ccagtgaatc gacaccaaac cagtgctgca aagaaggtcc gttattcaga
      841 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
      901 accacaatca ataaaataac gaacaatgcg gcggtcatcg ccatatgctg acggcgaaat
      961 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1021 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1081 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     1141 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttcaacggag tcaacgagta
     1201 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatggaacca ccgagtaaaa
     1261 atccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     1321 tgaggacaac ctaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     1381 ttaaaacgtc gacaaacgac gcgcgggtaa agcgcgccat cacggcggcc actgccgcgc
     1441 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccacg gtaggcagcc
     1501 agcccagttc aacggagaat acctgaatta aaagcatccc cagagcaaat gccggaaacg
     1561 aaatccccga caccgcaata gtcatactca atcgatccgg ccaacggtta cgccagacgg
     1621 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     1681 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     1741 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgatata
     1801 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     1861 gcccggcaat taatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca
     1921 ccgagacgat aaacagcgtc ggaatcaacc ccagtaagcg tttgataacg taattaagca
     1981 ttccactccc tgcgttgctt attgcaaatc cgcatcttca aagctgaagc cggtgtctgg
     2041 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     2101 cgggatccac ggcgactctt gccagatgat atcctgcgcc gccttataca agcgggtctt
     2161 ttccgccgga tcattagttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     2221 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttctgc gaggcaaaca gcggcgatag
     2281 agcccagtct gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     2341 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcagtcac
     2401 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     2461 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctt
     2521 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     2581 ggcgatactc ggcggtacca caccagtagc tggcgttgca tagcccgcga aggccacttt
     2641 caccagcgcc gggcggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     2701 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     2761 ctcgatattt ttgtttttct ccagcagtgc ggcctgctcg tagggaatgg ggaaagcaaa
     2821 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     2881 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccacgaatt ttttcacctt
     2941 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3001 accaatctcc ttgccatatt tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg
     3061 ggcaagaata ttaataaacg ctgagaatgg ctgtttgaga gtaatcttta ccgttgtcgg
     3121 atcgatcgct tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     3181 cggatcgctg gcccggtcca gattcgcttt caccgccgct gcgttgaaat cggtgccatc
     3241 ctggaattta atgccttccc gcaatttcac ggtgtaagta aggccgtcat cggaaacggt
     3301 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     3361 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattt gcgtcatacg gatcgagcgt
     3421 ggtgaagttc gatcctactg ccaccaccac atttttggca gcgaatgcac aagatgccat
     3481 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     3541 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     3601 aatgccccgg cccgacgcat tgcaacgaga cggctgcaac ttcttcgcca cgcagatgaa
     3661 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     3721 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcggatta tcaaagaccg
     3781 cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     3841 tccgctcaac cacggccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     3901 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgagacgg
     3961 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4021 ggcgctgacc gccggaaaac tcatgagggt aacgccaggc atgttccggt aacaggccca
     4081 cgcgctccag caaccacgca acgcgtgcag ccgcttcttt acctggcaat aaaccgtgta
     4141 cgcgcagcgg ttcgagaatc gaatcaccga tggtctgacg cgggtccagc gaagcgtaag
     4201 ggtcctgaaa aataaactga atatcgcggc gcaatgcctg aagtttgccg ggtgacaagg
     4261 tatcgattcg ctgaccgtta aagataattt cgccgccttg cgattcgacc aggcgcagca
     4321 acgcccgccc ggtggtggat ttaccgctgc cagactctcc caccagcgat agcgtttcac
     4381 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     4441 acaaaccgct gcgcaaaggg aaacgggtga ccagattacg cacccgtaaa acaggttcgc
     4501 catccaccac cgttttctgc tcgatgggtg gctcctgttt cgctggatgt tcaagcgata
     4561 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     4621 ctaacagcgc acgggtataa ggatgttgcg gtgcatgaaa aatctgttcg acgctacccg
     4681 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     4741 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     4801 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     4861 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     4921 agagttgatg cggataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     4981 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaatag
     5041 attcagcaat ctgttcaccc acagtaaata ccgggttcag cgatgtcatc ggctcctgaa
     5101 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     5161 gttcaattac ttcgcgactg cgccgctgca acagcatttt atcgcactgc actaaaccgc
     5221 ccgcctgttc caacaggcgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     5281 cgccaacaat tgccagcgtc tcaccacgtt gcaggctaaa agagagattg cggaccgcag
     5341 ctattttctg ctgctcctgc ataaaggcaa tattcagatt ttcaaccgcc agcacatcac
     5401 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctc tttcacggta gataccggtg
     5461 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     5521 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     5581 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     5641 gcggcgatat catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc ggtacaggaa
     5701 accgccacac tggcgttatt ggcatagcat ccggccccca ctaagggact atcgccaact
     5761 cgtccgggta atttattggt cattccgccc gtggacgtgg ctgccgccag attgccgtct
     5821 aaatctaacg ccacggcccc tacggtgccc attttttgtt tttcatccag tggcgcaccg
     5881 ctatggtcga ggacggttgc cccttcctcg cgcgctgcca ttagttgttc ataacgcaaa
     5941 ggtgtggaga aaatctccgg cgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6001 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagtcg ggcggcaaga
     6061 accggattac gcagatggct aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     6121 atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     6181 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     6241 tcgcccgcta ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     6301 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     6361 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     6421 aatatcagaa tttttatgaa tgactcattc aatttttgaa tatagaaaga tggatatggc
     6481 atgtaaaggc aggagccagc gctacgcccg aggtggcata cagtctgtgg cgtttttctg
     6541 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     6601 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccgc
     6661 ccaggaaacg ctgccactgg ttcagtgttt tggccgtatt ctggcgagcg atgtcgtttc
     6721 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     6781 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta
     6841 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     6901 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     6961 tactgctgaa gcgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7021 tgcggttgtt ttcccggcgg ggactcgcct gactaccgct gaactgccag tgattgcttc
     7081 gctagggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     7141 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg ataccaaccg
     7201 tctcgccgta cacctgatgt tagaacagtt gggatgcgag gtaatcaact tagggattat
     7261 ccgcgacgat ccccatgccc tgcacgccgc atttattgaa gccgacagcc aggcggatgt
     7321 ggtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     7381 agagctgggg gagatcgcct tctggaaact ggcgattaaa ccgggtaaac cgttcgcatt
     7441 cggtaaactt tctaatagtt ggttctgcgg cctgccgggc aacccggttt cagcgacgct
     7501 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaata ccgccagtgg
     7561 cctgcccgcg cgccagcgtg tacgcacagc gtcccgcctg aaaaaaacgc caggacgcct
     7621 tgatttccag cgcggcgtgc tgcaacgcaa cgccgatggc gaactggaag tgacgaccac
     7681 cggacatcag ggttcacata tatttagctc ctttagcctc ggcaactgct ttatcgtgct
     7741 ggaacgcgag cgcggcaatg ttgacgtggg cgaatgggtg gaagtcgaac cgtttaacgc
     7801 gttgttcgga ggcctgtaat ggcagaactc agcgatcagg agatgctgcg ctacaaccgg
     7861 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     7921 ctggtcgtcg gccttggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     7981 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctccaatct gcaacgccag
     8041 acgctgcaca gtgatgccac ggtcgggcaa ccaaaggtgg aatccgcccg tgacgccctg
     8101 acgcggatca acccacatat cgcgattacg ccagtcaatg cgctgcttga tgacgctgaa
     8161 cttgctgcga tgattgcaaa acacgatctg gtgctcgact gtacggataa cgttgcagta
     8221 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     8281 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     8341 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     8401 ccgttaatcg gcgtaattgg ttcgttgcag gcaatggaag caatcaaact gctggcaggt
     8461 tatggcaaac cagccagcgg gaaaatcgtg atgtacgatg cgatgacctg tcagtttcgc
     8521 gaaatgaaac tgatgcgtaa tccggggtgt gaggtgtgcg ggcagtaact gccacctcca
     8581 ggccagataa gacgcggcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc
     8641 cggttaaatc gacgttctgc caaacgctcc cagccagtcc tgttcaaact tcgccacagc
     8701 ggcatcaacc gccggatagc taatcatctg ttgtgccaca tccagcggca gagtaattga
     8761 ttcacatcct gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgcggctaa
     8821 cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat
     8881 accgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc
     8941 cagcgccgac agtagccctt gtgctgcgcc atataccgcg gttcccagcg tcggaatccc
     9001 ttccgctttt aacatcttaa tagctgccag cccctcagcg gtcaccggaa ctttcaccac
     9061 gatatccgca ataatagaac gcaatttacg tgcgtcatta accatccctt cagcagtggt
     9121 agccattacc tgggcaaaca gacgcccctg accgcccatc gcctcgtgaa gttgcggaag
     9181 cacaacctcc agcggttttt tacccgctgc gataatgctt gggttagtgg tcacacccgc
     9241 cagcggaaaa atacgtgaca gcgccgtcac cgcaacaacg tctgaagtat ccagatacag
     9301 ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc
     9361 tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa tgtttgaacg
     9421 aaacagagag gcaactatga ttttcaatat tcaacgctac tcgacccatg atggccccgg
     9481 tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc
     9541 ggaaagccgc gcccgcacgc aggatctgct gtatgaccca cgactctgtc tggaaggctg
     9601 taagctgtgc gctaagaccg caccggaagt gattgagcgc gcgttgaatg gtttgcttat
     9661 tcatcgggaa aagttaaccc cggagcattt tacggcgcta accgactgct gcccgacaca
     9721 ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcaa ccgttttgcg
     9781 cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttctggcg gtgaaccgtt
     9841 tatgcagccg gaaatggcga cggcgctgct gcaagccagc cacgaggccg gcattcatac
     9901 tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgccctatat
     9961 cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga
    10021 cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagccg gcaaaaaaat
    10081 cattatccgc gtgccactga ttcagggctt taatgccgac gaaacttctg tgaaagccat
    10141 taccgatttt gccgccgacg agctgcacgt tagcgaaatt cattttctgc cctaccacac
    10201 actgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct
    10261 tgatgcgcca gaactgctcg actttgccca gcagtatgcc tgccagaaag ggttaaccgc
    10321 gaccttacga ggataacaat catgacaaca ctaaaactgg atacgctcag cgaccgcatt
    10381 aaagcgcaca aaaatgcgct ggtgcatatt gtgaaaccgc cggtctgtac cgaacgagca
    10441 caacactata ccgagatgta tcaacaacat ctcgataagc caatcccggt acgtcgcgcg
    10501 ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaagcacga tgagttgatc
    10561 attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata taccgtctcg
    10621 tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggttt tgcggtgagc
    10681 gaagagaaca aacgcgttct gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag
    10741 gatcgctgct acggcatgtt taccgatgag caaaaaggtc tgctggcgac cggaatcatt
    10801 aaagcggagg gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctactg
    10861 ctggaaaaag gtcttgatgg tctgcgtgag aaagtggcgg aacgtcgctc gcacatcaat
    10921 ctgacggttc tggaagatct gcacggcgag caattcctga aagcgattga tatcgtgctg
    10981 gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgaaat ggccgcgacc
    11041 gaaacccgcg aaagccgtcg cgatgaactg ctgacgatag cagaaaactg cgatcttatc
    11101 gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgctactt catccagttg
    11161 attttgcaga tcgaatctaa cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc
    11221 tacccatact atcgctgtga cgttgaactg aaccagacac tggatcgcga acacgccatc
    11281 gagatgctgc atagctgctg gctgaaactg ctggaagtga acaaaatccg ctccggctca
    11341 cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg cgggcaaaat
    11401 ctggttgatg gtcaaccgat ggacgcggta aatccactct cttatgcgat cctcgaatct
    11461 tgcggtcgcc tgcgttcgac tcagcctaac ctcagcgtgc gttaccatgc gggtatgagt
    11521 aacgatttcc tcgacgcctg tgttcaggtg atccgctgcg gcttcgggat gccggcgttc
    11581 aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct
    11641 tacgactacg cagcaattgg ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc
    11701 tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggtggt
    11761 cgtgatgcca ccagcggcaa agtgttcctg ccacaagcaa aagcgttgtc agcgggtaac
    11821 ttcaacaact tcgatgaagt aatggacgca tgggatacgc aaatccgtta ctacacgcgt
    11881 aaatcaatcg aaatcgaata tgtcgtcgat accatgctgg aagagaacgt acacgatatt
    11941 ctctgctcgg cgctggtcga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc
    12001 gcgaaatatg actgggtttc tggcctgcag gtcggcattg ctaacctcgg caacagcctg
    12061 gcggcagtga agaaactggt atttgaacag ggcgcgattg gtcagcaaca gctggctgcc
    12121 gcactggcgg atgacttcga cggtctgact cacgagcagc tgcgtcagcg gctgattaac
    12181 ggtgcgccga agtacggcaa cgacgatgat actgtcgata cgctgctggc tcgcgcttat
    12241 cagacctata tcgacgaact gaagcagtac cataacccgc gctacggtcg tggtccgatt
    12301 ggcggcaact attatgcggg cacatcgtct atctccgcta acgtaccgtt tggcgcgcag
    12361 acgatggcaa cgccggacgg acgtaaagca cataccccgc tggcagaagg cgctagtccg
    12421 gcctccggta ctgaccatct cggccctact gcggtcattg gctcggtggg taaactgcct
    12481 acggccgcga ttctcggcgg cgtgctgctc aaccagaaac tgaatccagc aacgctggag
    12541 aacgaatctg acaagcagaa actgatgatc ctgctgcgca ccttcttcga ggtgcataaa
    12601 ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaagc gaaaaaacat
    12661 ccggagcagt accgcgatct ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg
    12721 ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatctcct
    12781 cagcccggcg gcgtcgtcgc cgggtcaaat cattcacatc atcaattccg ctctcacttt
    12841 cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt
    12901 tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagacatgg acggtacttt
    12961 tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcagtatc aggaacttaa
    13021 aaagcgcggc attgagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt
    13081 ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga
    13141 acatggcaag cagttgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg
    13201 cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt
    13261 cagcgaaaat gcccccgaag cattcgtcgc actgatggca aaacactacc atcgcctgaa
    13321 acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc
    13381 ggatgaacaa atcccgttag tgatcgacaa actgcacata gcgctggatg gcattatgaa
    13441 acccgtcacc agtggttttg gctttatcga cttgattatt cccggtctac ataaagcaaa
    13501 cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg
    13561 cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgagcaa
    13621 tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg
    13681 cgcgctgaat gtgattcagg cggtgctgga aaataaaccg ccgtttaatc tttgattatc
    13741 gccaattcag tttattgaga aagtgcatgt cattatgccg gatacggcgt aaccgcctta
    13801 tttggcatac aaaatcatga aaattcaata tattgcgata cactaccagg cctgataagc
    13861 gtagcgcacc aggcaatctt gcgtttgtca tcagtttcag tagccctgac catatcaggg
    13921 ctactgataa taacttactc gatcccttta ctacgcaggt aatcttcgta attaccgcta
    13981 aagtcgatca cgcgttccgg ggtgatttcc agaatacggg tcgccaggga gcttacaaac
    14041 tcacggtcgt gagaaacaaa gatcagcgtg ccctgataca gttccagcgc catgttcagc
    14101 gactcgatgg attccatatc caggtggttg gtcggttcgt ccatgatcag aatgttcggc
    14161 ttctgcatca ttaacttacc aaacagcatc cgccctttct ccccaccgga aagcactttc
    14221 gctggctttt tgatgtcgtc ctggctgaac agtaaacgac cgagaatgct gcgtaccgcc
    14281 tgctcgtcat cgccttcctg cttccactgg ctcatccatt cgaacacggt cagatcattt
    14341 tcgaactcat attcatggtc ctgggcgtag taaccaatgc gcgcgttctc agaccatttt
    14401 acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt tgatttaccg
    14461 acgccgttgg tacccagtac agccagtttt tcacccactt ccagcagcag gttgagattt
    14521 ttaaacagtg gaccgttatc aaacccttta gtcagacctt ccacttccag cgcgttacgg
    14581 aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga ggctttcacc
    14641 tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag
    14701 gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc tttcttcttg
    14761 gcgttatcag ccagcagacg ttcacgcgcc tgagtcgccg ccgtcatgta ctcatcgtag
    14821 ttgcccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt acaaaccatg
    14881 ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc gttcagcacc
    14941 tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga
    15001 atgtccggat cagcaaacag cgcctgcgcc agcagcacac gcagcttcca gccaggagca
    15061 acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag caacagttca
    15121 ccagcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttcacttc cagatcggcc
    15181 actttatagc cgtcttcttc actcatttcc ggcaaagcat agatgcggtc acgttcctgc
    15241 ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt gaactcttca
    15301 aaggcaaact gatcctgacg cagcttacca atgcgctcat tgggatcgag ggaaacgtta
    15361 cccaacgtcg gctctaagtc gccgccgagg atcttcataa aggtggattt accactaccg
    15421 ttcgcgccaa tcaggccgta acggttgccg ccgccgaatt tgacggaaat gttttcaaac
    15481 aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg cgtatcctga
    15541 aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc tgctcctcgc
    15601 ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc tgatttcact
    15661 gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa cccaatggcc
    15721 tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat tcgcagcggc
    15781 cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc tgccaaccga
    15841 cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta acactcagcc
    15901 gctggagtat tttgccgccg agtaccagat gggactttcc aatatgatgg aagcgaaccc
    15961 gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc agcagctgat
    16021 cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc gtctgtatta
    16081 ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc agttaggcaa
    16141 agacacgcct atcaactgga ccaccaaagt tgagcgtaaa aaagcaggcc cgacctggac
    16201 gccgaccgct aaaatgcacg cagagtaccg cgctgcgggc gaaccgcttc cggcggtcgt
    16261 tccggctggt ccggataacc cgatggggct gtatgcactc tacatcggtc gcctgtacgc
    16321 tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg
    16381 tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta cccgcgtaca
    16441 gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt atattgaagt
    16501 ccataacccg ctgtctacaa ccgaagccca gtttgaaggt caggaaattg tgccaattac
    16561 cctgacgaag agcgtgcaga cagtgaccgg tcagccagat gttgaccagg ttgttctgga
    16621 tgaagcgatc aaaaatcgtt ccgggatgcc ggttcgtctg aattaatctt caaaacgtaa
    16681 agcaaaggcg gactcataat ccgccttttt aatttgacag accttatccc gccgggatca
    16741 taaccaacaa aacatatccc accagcgccg cccacaacag catcggtatc gaataaagat
    16801 gaaaacgcca ccagatacgg cgatcgttcg ccatccgtaa cgcaatcaaa tttgccagcg
    16861 atccgggtaa taaaccaaag ccccccacgt ttaccgccca tgccagtaat aaagacggtg
    16921 gcacataatt gagcagcaaa atggtactcg gcacgttact gatcacctgc gataaaccga
    16981 tcgccgttaa ccataatccg ggttcggata aatgactcac gttacttaac accccctgca
    17041 aaactggcag ttgggttagt aaatggacgt cgataaacat cgccataaac accagcagca
    17101 gcgtccagtc cacactgagc accacacgac gcgccagcag cgcaaagccc gccgccacaa
    17161 tcaccagtcc ccacagctct tgttttaact ccagcgccgt cagaaagacg atatacagcc
    17221 ccagacaact ccacaccagc cgcggtttcc actccggcgt ttgcaccccc gtatggtatt
    17281 gcagcgcctt tccagggaaa caacaccagc acaacagcag gagtgtcagc atcattgcgc
    17341 cagccagcgg tgccatttgg gcaataaatc cggcaaacga aagaccagaa cgtccccaga
    17401 taagaatatt ttgcgggttg ccaattggcg tcagtagcga accagcgttg actgccagcg
    17461 cctcaaaaat aatcagccga ttaaccggaa tctcacacag tctttttagc gtgatagtca
    17521 gcggaacaac tataaacagt gcgacatcgt tggtcagaaa ggtagaaagc agcgctgccg
    17581 ccagtaccat aaacatcgcc agccgacgct ccgtagcaaa acggcgcacc attttgcgcc
    17641 ccagcacatc aaaataaccg cttaactcga cacctttggt cagcagcatc aggccgctta
    17701 aggtgatgat ggtgtgccag tcgatagcag caggccagga tttcggtgca aagggaacga
    17761 aaaaacttaa tccgatgcca acaagaatta ataactgaaa aaaacgatcg ccttgcagcg
    17821 tgcgtaaaaa aggcaggctc atttggcacc gtgtttctgg gtaaacaaac gaaaggcatc
    17881 cagcgtctct tcactaacat ggtgctccat accttccgcg tcgcgacggg cgatttccgg
    17941 actgacgccc aacaccaaca agaaattttc gactatctga tgtcgctcgc ggctttcctg
    18001 cgccagcttc tctccttctg ccgttaaaaa cacgccacgc caggggatca tttcaatcag
    18061 ccccatggtt gccagtcgct taagcatttt agccaccgtc ggttgcgaaa cccccagacg
    18121 agcagccatg tccacctgac gagcttcccc cacttccctg atcaagtcag aaatcagctc
    18181 aacgtaatca tcaatcagct cgcgccgatg cgcctctctg acctggcgga acccttcaac
    18241 gtgctcttcc acgttcacta actgcgtcac tttttttgct gttggcgtac ctgcgcgacg
    18301 actcattgtg cttcctcatt atggtgacgc atacatagcg ttctcatttc gtgcgcacat
    18361 tgtaaaccag agttgcgagg gtacaaaaaa ttaacgtttt agcaatagct atataatata
    18421 gcctgtgcta tatctgtata taatgcaatc atccctcaag gatcgatggg attagcaagt
    18481 aaggaggtct tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta
    18541 aagtacggtt aatgctgctc tctatgttgt gcgatatggt caacaacaaa ccgcagcagg
    18601 ataaaccttc cgataaatag cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag
    18661 gcacaacgcg ttttggtctg aaaaacccca ctttttgtcg gatttgcaat ccccttcgca
    18721 aaagatttgt tcgtcggtag ttgacctgaa cagcagctcg ctctatcttc ttgcagccct
    18781 gcgtatattg cggctcgcgg atgcggaccc ctttcccctc ttcacgcact cttgcaggta
    18841 ttgacccttg acgccagggt aagcacatgg cgtttgttac gatagtggca tatgaattta
    18901 accctcaaag aatcgcttgt tacccgtagc cgggtattta gcccgtggac tgcgttctac
    18961 tttttacagt cgctattaat taacctcggc ttaggttacc ccttcagttt gctctacacc
    19021 gctgcgttta cggctatttt gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt
    19081 ctggtcggta tcagctcgct ggtggcggct tgttatttcc cttttgctca ggcctacggc
    19141 gcgcctaact tcaatacatt gctggcattg cactccacca atatggaaga gtcgaccgaa
    19201 atcctgacga tttttccatg gtacagctac ctggtcggca tatttatttt tgcgctcggc
    19261 gtaatagcga tcaggcgaaa aaaagagagt gaaaaagcgc gctggaatac cttcgacagc
    19321 ctgtgcctgg tattcagtgt agcgacattt tttgttgctc ccgtgcaaaa cctggcctgg
    19381 ggcggcgtat ttaaactgaa agatactggc tatccggtat ttcgttttgc taaggatgtc
    19441 atcgtcaata ataacgaggt gattgaagag caagaacgga tggcaaaact ttccggaatg
    19501 aaagatacct ggacggtcac tgccgttaag ccgaagtatc agacctatgt ggtggtgatc
    19561 ggagaaagcg cgcgtcgcga tgccctcggt gcctttggcg gtcactggga caataccccg
    19621 tttgccagca gcgttaacgg tttgatattt gctgactaca ttgccgccag tggctcgacg
    19681 cagaaatcgc ttggcttaac gctcaatcgc gttgtcgatg gcaaaccaca gtttcaggat
    19741 aactttgtca ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc caaccagggt
    19801 caaatcggcg aatacgatac cgccatcgcc agcatcgcca aacgagcaga tgaagtgtac
    19861 ttcctgaaag aaggtaattt tgaagcagat aaaaacacga aagacgaagc gttactggat
    19921 atgaccgctc aagtgctggc gcaagagcac tcgcaaccac agctgattgt tctacatctg
    19981 atgggctcgc atccgcaggc ctgcgaccgg acacaaggaa aatacgaaac ctttgtgcaa
    20041 tcgaaagaaa cgtcgtgcta tctctatacc atgacgcaaa cagacgattt actgcgcaag
    20101 ctgtacgatc agttacgcaa cagcggcagc agcttctcgc tggtttactt ttctgaccac
    20161 ggtctggcct ttaaagagcg cggcaaagac gtgcaatacc ttgctcatga tgataagtat
    20221 cagcaaaatt tccaggtgcc ttttatggtc atttccagcg acgataaagc gcatcgggtg
    20281 attaaagccc gccgctctgc caatgacttc ttaggctttt tctcgcagtg gacgggaatt
    20341 aaagcgaagg aaataaatat caaatacccg tttatttctg agaagaaagc cgggccgata
    20401 tacatcacca acttccagtt acagaaggtg gattacaacc atctcggaac cgataatttc
    20461 gacccgaaac cttaaaacaa aaaaatccgc cccgagaggc ggatttttat atcaccgaag
    20521 tgattagaaa cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    20581 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    20641 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    20701 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    20761 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    20821 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    20881 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    20941 tgcgtaaccg ccagttacag tagaagtcgc agctacggaa gtacctgcgg tgaaagccag
    21001 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    21061 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttatgat
    21121 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    21181 aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga
    21241 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    21301 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    21361 tacttaggtt tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    21421 ctggttacca atagtcatat tgctcgttgc catggcgtct attcagggtg gagcctcgtt
    21481 agctaagtca ctttttcctc tggtgggcgc accgggggtc actgcgctgc gtctggcatt
    21541 aggcacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    21601 acgattaccg ctgttgtttt acggcgtttc gctgggtgga atgaattatc ttttttatct
    21661 ttctattcag acggtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    21721 ggtggcactg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    21781 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    21841 tgcactggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    21901 tggagcggaa catggtcctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    21961 cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    22021 gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    22081 cacccgtttg ccaacacgca catttggtac gctaatgagc atggaacctg cgctggctgc
    22141 cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg
    22201 cgctatcatc gccgcttcaa tgggttctac gctgacagta cgcaaagaga gcaaaataaa
    22261 agaattagac ataaattaaa tttacat
//
LOCUS       DAZFRG010000060        22206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFRG010000060 DAZFRG010000000
VERSION     DAZFRG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.060"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1410
                     /gene="tnaA"
                     /locus_tag="ACHKUC_001789"
     CDS             <1..1410
                     /gene="tnaA"
                     /locus_tag="ACHKUC_001789"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO6618054.1"
                     /translation="NFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDV
                     FIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRG
                     AEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTG
                     VRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKY
                     DIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYRYADMLAMSAKKDAMVPMGG
                     LLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIA
                     QVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAV
                     EIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGL
                     TFTYEPKVLRHFTAKLKEV"
     gene            1502..2749
                     /gene="tnaB"
                     /locus_tag="ACHKUC_001790"
     CDS             1502..2749
                     /gene="tnaB"
                     /locus_tag="ACHKUC_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO6618055.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKKKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2881..4056
                     /gene="mdtL"
                     /locus_tag="ACHKUC_001791"
     CDS             2881..4056
                     /gene="mdtL"
                     /locus_tag="ACHKUC_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO6618056.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLNFPWQSLF
                     WTMAIMGVAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCCSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4031..4990
                     /gene="yidZ"
                     /locus_tag="ACHKUC_001792"
     CDS             4031..4990
                     /gene="yidZ"
                     /locus_tag="ACHKUC_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO6618057.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5147..5896
                     /locus_tag="ACHKUC_001793"
     CDS             5147..5896
                     /locus_tag="ACHKUC_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO6618058.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSVSYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5918..6484
                     /gene="yieF"
                     /locus_tag="ACHKUC_001794"
                     /gene_synonym="chrR"
     CDS             5918..6484
                     /gene="yieF"
                     /locus_tag="ACHKUC_001794"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO6618059.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVLQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQSTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6538..7875)
                     /gene="adeP"
                     /locus_tag="ACHKUC_001795"
     CDS             complement(6538..7875)
                     /gene="adeP"
                     /locus_tag="ACHKUC_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO6618060.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8042..8707
                     /gene="yieH"
                     /locus_tag="ACHKUC_001796"
     CDS             8042..8707
                     /gene="yieH"
                     /locus_tag="ACHKUC_001796"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO6618061.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8774..9247
                     /locus_tag="ACHKUC_001797"
     CDS             8774..9247
                     /locus_tag="ACHKUC_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO6618062.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(9329..10051)
                     /locus_tag="ACHKUC_001798"
     CDS             complement(9329..10051)
                     /locus_tag="ACHKUC_001798"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6618063.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10066..11235)
                     /locus_tag="ACHKUC_001799"
     CDS             complement(10066..11235)
                     /locus_tag="ACHKUC_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO6618064.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11262..12878)
                     /gene="bglH"
                     /locus_tag="ACHKUC_001800"
     CDS             complement(11262..12878)
                     /gene="bglH"
                     /locus_tag="ACHKUC_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO6618065.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(12964..14358)
                     /gene="bglB"
                     /locus_tag="ACHKUC_001801"
     CDS             complement(12964..14358)
                     /gene="bglB"
                     /locus_tag="ACHKUC_001801"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO6618066.1"
                     /translation="MKAFPETFLWGGATAANQMEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTAFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFEW
                     YAEVIKTRGLSLKK"
     gene            complement(14377..16252)
                     /gene="bglF"
                     /locus_tag="ACHKUC_001802"
                     /pseudo
     CDS             complement(14377..16252)
                     /gene="bglF"
                     /locus_tag="ACHKUC_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(16386..17222)
                     /gene="bglG"
                     /locus_tag="ACHKUC_001803"
     CDS             complement(16386..17222)
                     /gene="bglG"
                     /locus_tag="ACHKUC_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO6618067.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(17508..18233)
                     /gene="phoU"
                     /locus_tag="ACHKUC_001804"
     CDS             complement(17508..18233)
                     /gene="phoU"
                     /locus_tag="ACHKUC_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO6618068.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(18248..19021)
                     /gene="pstB"
                     /locus_tag="ACHKUC_001805"
     CDS             complement(18248..19021)
                     /gene="pstB"
                     /locus_tag="ACHKUC_001805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO6618069.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(19112..20002)
                     /gene="pstA"
                     /locus_tag="ACHKUC_001806"
     CDS             complement(19112..20002)
                     /gene="pstA"
                     /locus_tag="ACHKUC_001806"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO6618070.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKRKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20002..20961)
                     /gene="pstC"
                     /locus_tag="ACHKUC_001807"
     CDS             complement(20002..20961)
                     /gene="pstC"
                     /locus_tag="ACHKUC_001807"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO6618071.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21048..22088)
                     /gene="pstS"
                     /locus_tag="ACHKUC_001808"
     CDS             complement(21048..22088)
                     /gene="pstS"
                     /locus_tag="ACHKUC_001808"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO6618072.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
BASE COUNT         5579 a         5690 c         5283 g         5654 t
ORIGIN      
        1 aactttaaac atctccctga accgttccgc attcgtgtta ttgagccagt aaaacgtacc
       61 actcgcgctt atcgtgaaga ggcaattatt aaatccggta tgaacccgtt cctgctggat
      121 agcgaagatg tgtttatcga tttactgacc gacagcggca ccggggcggt gacgcagagt
      181 atgcaggccg cgatgatgcg cggcgacgaa gcttacagcg gcagccgcag ctactacgcg
      241 ttagccgagt cagtgaaaaa tatctttggt tatcaataca ctattccgac tcaccagggc
      301 cgtggtgcag agcaaatcta tattccggta ctgattaaaa aacgcgagca ggaaaaaggc
      361 ctggatcgca gcaaaatggt ggcgttctct aactatttct ttgataccac gcagggccat
      421 agccagatta acggctgtac cgtgcgtaac gtctatatca aagaagcctt cgatacgggc
      481 gtgcgttacg actttaaagg caactttgac cttgagggat tagaacgcgg tattgaagaa
      541 gttggcccga ataacgtgcc gtatattgtt gcaaccatca ccagtaactc cgcaggtggt
      601 cagccggttt cactggcaaa cttaaaagcg atgtatagca tcgcgaagaa atacgatatt
      661 ccggtggtca tggactccgc acgctttgcc gaaaacgcct atttcatcaa gcagcgtgaa
      721 gcagaataca aagactggac catcgagcag atcacccgcg aaacctacag atatgccgat
      781 atgctggcga tgtccgccaa gaaagatgcg atggtgccga tgggcggcct gctgtgcatg
      841 aaagacgaca gcttctttga tgtgtacacc gaatgcagaa ccctttgcgt ggtgcaggaa
      901 ggcttcccga catatggcgg cctggaaggc ggcgctatgg agcgtctggc ggtaggtctg
      961 tatgacggca tgaatctgga ctggctggct tatcgtatcg cgcaagtgca gtatctggtc
     1021 gatggtctgg aagagattgg cgttgtctgt cagcaggcgg gcggtcacgc ggcattcgtt
     1081 gatgcaggta aactgttgcc gcatatcccg gcagatcagt tcccggcaca ggcgctggcc
     1141 tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa ttggctcttt cctgttaggc
     1201 cgcgatccga aaaccggtaa acaactgcca tgcccggctg aactgctgcg tttaaccatt
     1261 ccgcgcgcaa catatactca aacacatatg gacttcatta ttgaagcctt taaacatgtg
     1321 aaagagaacg cggcgaatat taaagggtta acctttacct acgaaccgaa agtattgcgt
     1381 cacttcaccg caaaactgaa agaagtttaa ttaaaacact cagagtggct ataaggatgt
     1441 tagccactct attaccctac atcctcaata acaaaaatag ccttcctcta aaggtggcat
     1501 catgactgat caatcagaaa aaaaacactc tgcattttgg ggtgttatgg ttatagccgg
     1561 tacagtaatt ggcggtggga tgtttgcttt acctgttgac cttgccggag cctggttttt
     1621 ttggggcgct tttattctta ttattgcctg gttttcaatg ttacattcag ggctattatt
     1681 attagaagca aacttaaatt atcccgtcgg atccagtttt aacaccatca ccaaagattt
     1741 aatcggtaac acctggaaca ttatcagcgg tattaccgtt gccttcgttc tctatatcct
     1801 cacttatgcc tatatctctg ctaatggtgc gatcattagt gaaacgatat caatgaattt
     1861 gggctatcac gctaatccac gtattgtcgg gatctgcact gccattttcg ttgccagcgt
     1921 attgtggata agttcgttag ccgccagtcg tattacctca ttgttccttg ggctgaagat
     1981 tatctccttt gtaattgttt ttggttcttt cttcttccag gtcgattact ccattctgcg
     2041 cgattccacc agcaccactg cggggacgtc ttacttcccg tatatcttta tggctttgcc
     2101 ggtgtgtctg gcgtcatttg gtttccacgg caatattccc agcctgatta tttgctatgg
     2161 caaaaagaaa gacaaactaa tcaaaagcgt ggttttcggt tcgctgctgg cgctggtgat
     2221 ttatctcttc tggctctatt gcacgatggg gaatatcccg cgagaaagct ttaaggcgat
     2281 aatctcctca ggcggcaacg ttgattcgct ggtgaaatcg ttcctcggca ccaaacagca
     2341 cgggattatc gagttttgcc tgctggtgtt ctccaactta gctgttgcca gttcgttctt
     2401 tggtgtcacg ctggggttgt tcgattatct ggcggatctg tttaagattg ataactccca
     2461 cggcgggcgt ttcaaaaccg tgctgttaac cttcctgcca cctgctttgt tgtatctgat
     2521 cttcccgaac ggcttcattt acgggatcgg cggtgccgga ctgtgtgcca ccatctgggc
     2581 ggtaataatt cctgctgtac tggcaatcaa agcccgcaag aagtttccca atcagatgtt
     2641 cacggtctgg ggcggcaatc ttattccggc gattgtcatt ctctttggca taaccgtaat
     2701 tttatgctgg ttcggtaacg tctttaacgt gttacctaaa tttggctaaa tccttcagga
     2761 agccagccat tcgctggctt cttgcctgtc aggaaatcac ttatgtccaa atggcaactc
     2821 gcctgattct ccttcaccac gtatgctttg cgtcacctta ctatcaggac gctttagccc
     2881 atgtcccgct ttttgatttg tagttttgcc ctggttttac tttatcccgc agggattgat
     2941 atgtacctcg ttggtttacc gcgcatcgcc gccgatctca atgccagcga agcgcagttg
     3001 catattgcgt tctccgttta tctggcgggg atggcggctg cgatgttatt tgccggtaaa
     3061 gtggccgatc gttcagggag aaagcctgtc gccatacccg gcgcggcgct atttattatc
     3121 gcctcggtgt tctgttcact ggctgaaacc agcgcgttat ttcttgcagg gcgatttcta
     3181 cagggactgg gcgcgggctg ttgttacgta gtggcgtttg ccattttgcg cgacacgctg
     3241 gatgatcgac gtcgggctaa agtgctgtcg ttactcaacg gcattacctg catcattccg
     3301 gtgttagcgc cagtgctcgg acatctgatt atgcttaact tcccatggca gagtctgttc
     3361 tggacgatgg caataatggg tgtcgcggta ctgatgttgt ctttgtttat tttaaaagaa
     3421 acgcgccctg cggcccccgc agcttcggac aaaccacgag aaaatagcga gtcactgctt
     3481 aaccggtttt tcctcagccg tgttgttatc accaccctca gcgtttcggt gatcctcacc
     3541 ttcgtcaaca cgtcgccagt attgctgatg gaaatcatgg gttttgagcg cggagaatac
     3601 gccaccatta tggcgttgac cgctggcgtc agcatgaccg tttcattctc cacgccgttt
     3661 gcgctgggaa tttttaagcc acgtacgttg atgatcacct cacaggtgtt attcctggcg
     3721 gcggggatca cccttgccgt ttcaccttcc catgcggttt cgctgtttgg tatcacgctg
     3781 atttgcgccg gtttctcggt aggttttggt gtggcgatga gtcaggcgtt agggccgttt
     3841 tcattacgcg cgggcgtagc cagctcgacc ttaggtattg cgcaagtttg ctgttcgtca
     3901 ctgtggattt ggctggcggc ggtggttggt atcggcgcat ggaatatgct gatcgggatt
     3961 ctgattgcct gtagcatagt aagcctgttg ctgattatgt tcgtcgcgcc tggacgcccg
     4021 gttgccgctc atgaagaaat ccatcaccac gcttgatctc aatctgctgc tctgtctgca
     4081 actgctgatg caggagcgca gcgtgaccaa agcggcgaag cggatgaacg tgaccccttc
     4141 ggcggtgagt aagtcgctgg caaagttaag agcgtggttt gacgacccgc tctttgtgaa
     4201 ctcaccgctg gggctgtcgc ccacaccgct aatggtcagc atggagcaaa atctggcgga
     4261 gtggatgcaa atgagcaacc tgctgctgga taaaccgcac caccagacac cgcgcggcct
     4321 gaagtttgag ctggcagcag aatcaccgct aatgatgatc atgcttaatg cgctgtcgaa
     4381 acggatctac caacgttacc cgcaggcgac catcaaatta cgtaactggg attacgattc
     4441 cttagatgcc attactcgtg gtgaagtgga tattggtttt tccggtcgcg aaagtcatcc
     4501 ccgttcgcgg gagctgttaa gctcgctacc gttagccatt gattatgaag tgctgtttag
     4561 tgatgtgccc tgcgtctggt tacgcaaaga tcatccggca ctgcatgaag catggaatct
     4621 ggacaccttt ttacgttatc cgcatatcag catttgctgg gaacagagcg atacctgggc
     4681 gctggacaat gtgttacagg agctggggcg cgaacgcacg attgctatga gcctgccgga
     4741 attcgagcag tcactgttta tggcagcaca gcccgacaat ctgctactgg cgaccgcgcc
     4801 gcgctactgc cagtactaca atcaactcca ccaactgccg ttggttgctc ttcctctccc
     4861 gtttgatgaa agccagcaaa aaaagctgga agtccctttt acccttctgt ggcataaacg
     4921 gaacagccgt aatccgaaga tcgtctggtt acgggaaacc attaaaaatc tttacgcgtc
     4981 gatggcataa ccgaatcgaa tgaaacgcga cccaatttca caatgaaatg taaaaaagtt
     5041 gtaataaact tatctgaatc gaacttttag ccgctttagt ctgtccatca ttccagtaaa
     5101 tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg cgactcactt
     5161 tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct cccagtgtca
     5221 tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt ccagaggttt
     5281 actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa tcgtcaccct
     5341 gccgaaagga aaaccggttt tcagcgataa aaatttgcct tcgttttctg tttcctatgc
     5401 cgggaatatg gtcggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg atatggaact
     5461 acagcgtgcg acacgcgggt ttcatagccc acacgcgccc gataaccaca ccttttccag
     5521 caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg cgcagctcat
     5581 cacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg atccgcgcga
     5641 tctgcaacta ctgcccattg ccggacgcct gaaatgtgct catgtaaatc atgtagaagc
     5701 gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgccca cgattgaaaa
     5761 gctcagtgtc tgggagttag atggcaaaca tggctggaaa agcctgcccg atattcacag
     5821 ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta ccgtgaaggc
     5881 tttttcgcca aattgataga caaccacagg agtcatcatg tctgaaaaat tgcaggtggt
     5941 tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac gtaccctgcc
     6001 gaaaattgct ccggcgagca tggaaatcaa tgcgttacct tccattgccg acattcccct
     6061 gtatgacgct gacgtactgc aggaagacgg ttttccagca acagtagaag ctctggcgga
     6121 acagatccgt caggctgacg gtgtggtaat cgtcacgccg gaatataact actcggtacc
     6181 gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac cgctggcagg
     6241 taaaccggta ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc gctgtcagta
     6301 tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc cagaatttat
     6361 gggcggcgtg attcagaaca aagttgatcc gcaaaccggg gaagtgattg atcagagtac
     6421 gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc gagttaagat
     6481 ctaaataaaa aacccgccag caatcatgca tggcgggttt ttaacgcgct atcgatttta
     6541 gtgagcgtcg ataaagacaa tcttcaggat aaacagcagc gcaacgatga ttacgcacgg
     6601 gctaaggtca cgcagacgtc cggtaccaat cttcatcacg cagtaggaga taaagcccag
     6661 cgcgatacct tcagtaatcg agaagctgaa cggcatcatc acagcggtaa taaacgccgg
     6721 aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca tcaacacgcc
     6781 aacgtaaatc agcgcaccag ctgcagcgta gcctggcacc atccccgcca gcggcgacag
     6841 gaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg tacgaccgcc
     6901 aacggaaacg ccggaagaag attcaatata cgccgtaaca gaagaagtac cgataaacga
     6961 accggtcacc gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg ggaatttacc
     7021 tttctcatca gccagacctg ctttatcggt cacgccaatc agcgtaccgg aggagtcaaa
     7081 caagttgacc agcatgaaag agaaaatcac ccctgccagc ccgaggttaa acgacccggc
     7141 taaatctaca tgacccacga cagtcattac gctcggcggc gcagaaacga tgccattata
     7201 gtggacatca cccagcatcc agcccagcag cgttgtcacc acgatggaaa ccagcaccgc
     7261 cgcgtgaata ttgcgcgagg ccagaatggc gatgatgaag aagccgagga tccccagcag
     7321 cacgctgtga gaagtcagat taccgatgct caccagcgtt tccgggttag cgacaatcac
     7381 acctgcgttt ttcagcccca tcatgccaat aaacagaccg ataccactgg tgatgcccac
     7441 acgtaggctt accgggatgt tggctatcat ccagtagcga acgcggaaaa tcgtcagtaa
     7501 cagcagacca atcgcgcccc agaagattgc gcccatcccg acttgccacg gcaagcccat
     7561 cgcctgtaca acgacaaaag cgaagaacgc attcaggccc attgccggtg ccagtgcaac
     7621 tggcaggtta gcaaacagcc ccatcataat actgccgaat gcagcaatca gacaggtcgt
     7681 gacgaagacg gcgctggtat ccatgccagc aacgccaaga atttgcgggt taacaaaaac
     7741 gatgtaaacc atcgtcagga aggtggtaaa accggcgatc acttcggtcc gtgccgtcgt
     7801 gccatgttcg cgcagtttaa acacgcgttc cagcatcccc tggccagaag cctgggttgt
     7861 gtgttgttga ctcattatct atttccgaac aaggagggaa aatccgtcgc tatcgtatac
     7921 caaaatgaga caatatggac ggctgaagat aacttttttt cattgtattt gcttatacgg
     7981 caacgattgc gtcatgcaaa ttggcatgac gaaaacgtta aactaacgat aaaggaaaga
     8041 catgtcccag atagaagcgg tatttttcga ctgcgacggt acgctggtcg atagcgaggt
     8101 catttgctct cgcgcctatg tcacgatgtt cagggaattt ggcattcacg ttgacctgga
     8161 agagattttt acccgtttta aaggcgtaaa actctacgaa atcatcgaca tcatcagtaa
     8221 agaacagggc gtgacgatgg tccaggccga tgccgaacac gtttaccgcg cagaagtagc
     8281 acgtctgttt gataccgaac ttgaggacat cgcaggagcg aacgaattgc tggcaagcat
     8341 taacgtgccg atgtgcgtcg tctctaacgg tccggtcagc aaaatgcagc attcgcttgg
     8401 taagctcaac atgctgcact acttcccgga aaaactgttc agcggctatg atatccagcg
     8461 ttggaagccc gatccggcat taatgttcca tgcggcaaaa gcgatgaatg tgaacgttga
     8521 aaactgcatt ctggtcgatg actccagcgc aggtgcgcaa tcagggatag atgccggaat
     8581 ggaagtgttt tacttctgcg ctgacccgca caataaaccg atcgttcacc cgaaagtcac
     8641 cacctttacc catctttcgc aattaccgga actgtggaag gcgcgtggtt gggatattac
     8701 ggcatagtta ttcacactcc cttcacttac tccacttaaa ttggcacgca aaggtaagta
     8761 aagggagttt gatatgtctg tttcacgtcg ggtaattcat cacggactgt attttgcggt
     8821 tttagggccg ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc
     8881 gctgattctt ttggtaataa tacaggtact tccattgttt ctcttaatgt cgataactac
     8941 gggagctatt cctgcaatgt taactggcgt aatggtcgcc tgtctgccgg aaaagatcgg
     9001 gtcacagaaa cgttatcgtt gtctggttgg cggcataggt ggagtcgtta tcaccgagat
     9061 ctattgcgct gttatcgtac atattaagga catggcttcc tcagcgttat ttgaaaacat
     9121 tctttctggt gaaaatctcg ttgtccgcat catcccagca ttgctggcag gcgtggtgat
     9181 gagcagaatc attacacgtc tacccggatt ggatatttca tgtcctgaaa cagactcttt
     9241 aagctaagcg gaatgtttta tctatttgtc attaaatgtt ggagaacagg ccggagcgta
     9301 attcacacat ccggccttat ttttaagctt aaccgagcgc taattctgcg gcagcggctt
     9361 tatcagcgat taccatcagc gaaggatgca gctgcaacac ggaagctgga acatcttcgg
     9421 taaccggacc ttgcagaaca tttttcagtg cttgcgcttt acctgcgcca ctgacaatta
     9481 tcagcaagtt tttcgccgcc atgatgcttt tcggccccat cgtcacgtag ctgtcaggca
     9541 ctaatgaaaa gtcgccgccc aactcgccat gcgccacaat atcaaccatc tctccctgga
     9601 tcgggaactc cacggtttgc tcatggaaat gggtcgtatt tggcagattg ccacaaaaat
     9661 gaccatctac acctaatccc aacaccacca aatccaggcc gccttcacgc gccagtttct
     9721 gatcatgctc gcggtagtta tcaatagtga gcttctggat attctcttct ttgatccccg
     9781 caggggtgaa aaacagatta cgcagattgg taatcgttac gccttcccct tctttaccgc
     9841 gaaatggaat ttcatcgaaa ttgtagaaat agcagttatc gtaccagggc ttacctttaa
     9901 ccagagtgat gagatattcg tacatgcctt tgggcgtgct accggcggta attgccaggt
     9961 taacgcgacg catctttgac atataaccaa gcagatgatg cgccgcgaca cggctcattt
    10021 cctggtaatc ttcggtaatg attaatttca tcttaatgcc ttatattatt tgaataattt
    10081 ctgtacgaag gctgcatagg caggccgcca gacatccatt tcatggttca gacccggata
    10141 ttcctggtaa tcaaacttaa tttttttctg ctcaagctca gttttcagcc cggcgatatc
    10201 cttgccggtt acaacatctt tatccccaac aaccacagta aaattacgta gttgctggtt
    10261 aatagctgcc ggctcgttca gccgggcagc gaccccttca tctggtacgg tcgtcgtggt
    10321 aacaccactg aatgtggcca gccagccaaa gctttccaga tgattcattc ccgataccag
    10381 tgcctgatac ccaccttgtg aaagccctgc cagtgcgcga ccatcggcat ctttacggac
    10441 attaaaacgc ttgctaatca gcgggataat atcgttcatc agttcgcgat ctgccgcttt
    10501 agcatttaac ggataaaaga ctttacgtct ctcctgagga acgaaatctt cgggaataat
    10561 gcccttcgca tcggtttcgg tatccgggat caccaccagc atcggtttaa ttttcccttc
    10621 agccagcagg ttatccatga tttgcgggat acgcccctga tcaatagcgg aacgtccggt
    10681 atcaccaaag ccgtgataga aatagagcac tggcaaaggc tcgcctatgc cggtgtatcc
    10741 tggcggggtc cagacataca tctgacgttc ggattgcaat gcgttggagt ggtaagttat
    10801 ggcgatcaaa tcaccatgca caacagaacg tgtatccaga taactgcccg gtaccagaat
    10861 catactggag ttaacctgac gctgaggctt agtcattgcc gtgcctgtat caatgctgcg
    10921 tacaccatca acattgaaaa aatactcgta cagattgcct ttcaggacag gcgtgcgcca
    10981 cgaccagact cccgcttcat ctttggtcat cgggtgaata ttgtccggaa ccggaacacc
    11041 caccactacg gagacatttt ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc
    11101 ggtattgacc tgagtcacat actgtttcac cggaatagtg ggtgccgggc tggctggcat
    11161 atccgctgct atggcccatt gagcagaaat accgctggca atagccagcg ttaaagcagc
    11221 aatttttata ttcatcgtaa tattcctttt attaagttcc attaccacca gatttcagcc
    11281 tgggcaccga cagcaaactg gtcgtcttta ttgtcttcaa aggtgaagcc atccagttca
    11341 ttttccagag ctttgatata cgtagcgtag aaacgaattt ccgggcgcga ggtcaacata
    11401 ctggtattga ctttaaaggt atggaagagc gtggttttat aaccagattc attatattta
    11461 ttgctattcg catctttgtt ttgctgggtg aaatagccca gttcaacacc tgtctggtta
    11521 tattggtccc aaatataggc cgggcgaaca accgcacgaa tagattcgaa atcagaatgt
    11581 gcgcctgttt cgtagctata aatatcgtta ccgaaggagt aaacaatcgc gttagccaca
    11641 ataaaatgat cgccgatata agcttcgccc tgcgaagtca gacgaactgc agttccgcct
    11701 gtatgatcac cataataacg accattaaag gtagtaaatg gacttgcacc agcataacga
    11761 ccaaagttac tggcgataga gttattcgca accaggaagg agaattcgtt gaagccaccc
    11821 ttatcaaatt tctgcgttaa agatgtacca aacatccagg tatctttcca gtcataatac
    11881 ccgttattat cctgattatc tttttccgat gcgctttcat ttgctgagac ataacgaccg
    11941 ctcaccatta aggtggcttt atcccataac gggatatctt tatagcgtaa atcaatggta
    12001 ttggtattaa tctgctgttt gttttgcagg ctgcgatcgt aatcatcgac atcttcacga
    12061 accagcgcga tatcaatttt acccggaccg actttccagt tttccagacc tacacctgcg
    12121 gcagcatcgg tacgctgcgt tttccagtca agcatctgga tttcaatttt cggcgcaccg
    12181 tgtttaccca cccagaaatc agcctctggc gcaaagggca ggaaaccttt ggtggtaaca
    12241 tacatatcag agaactgcat aaagttctcg ccaccggcgt tatcgccaaa ccagccagta
    12301 gagtactgct gacccacgtt accatccatc atcacgatgg catcaacccg tttgccgttt
    12361 tcgttgtaga cacgttgttt taactgcaaa tcaaaccagc cggagtattc gttaccaaag
    12421 cggcccagag aaccaattgc ccatgattta ggtgaaccat gagaggctgt tccccaacca
    12481 gaacggtagt agccgttgta actaaagccg atttcatctt tcacaaattt actgaaatct
    12541 ttcagagtca tggaagaata aatcgagcct gtctcactgg catttttctc ttcattttta
    12601 accagtacag catcaggctt tgcggcacga ctggtcggga atggattggc ggcataccct
    12661 tgatcattcg tacttacgct acgattcacc gtcgctggcg tatacttttt cttctcttca
    12721 tctttatatt ttttcagttc gctttgcgtt tctcttaacg ccttttctaa tagctcaagg
    12781 cgttgttcca ctgttaatga ttcagccaat gattgtgcgg aaaaggctaa cggtgccatt
    12841 aataagatgg cagaggtaat aatatttcgt ctaaacataa tatcccttta tggtgcaaag
    12901 aaagaattaa cgcatcgcat ccagactgtt ctgaatgcga cgatatttaa ggtgctttat
    12961 tggttatttt tttaatgata gcccccgagt tttgatcact tctgcatacc attcgaagct
    13021 tttcttgcgc gttcttgtga ggcttccttc accattatca tcacgatcca cataaataaa
    13081 gccgtagcgc ttagacattt gtgaatgaga tgcactgact aaatcaattg gcccccaact
    13141 ggtgtacccc ataatatcca caccatcggc aatcgcttca tttacctgta caaggtgatc
    13201 gtttaaatag gcaattcgat aatcgtcctg tatcgaacca tccacttcaa cgctgtcttt
    13261 tgcgcctaat ccgttctcga caataaataa cggtttttga taacgatccc aaagcgtatt
    13321 taacagaatc cgtaatccaa ccggatcaat ttgccacccc cactctgaac ttttcagatg
    13381 cggatttggg atcatattca gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg
    13441 ggaaacacaa ccggtcatgt aataactaaa agagatgaaa tctacggtat gttttaaatc
    13501 ttctgcgtca ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacgctgcat
    13561 atagccggga tactgaccac gcgcctgaac atcaccaaag aacatccagc gccggttctc
    13621 ttccatggcc tgcaacatat cctgtggctg gcaggtgagg gggtaaacca gcccaccgag
    13681 aagcatattg ccaattttcg cttcggggat caggctatga caggctttaa ctgcccgcgc
    13741 actggcaacc agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc
    13801 cagccccacg cccgtgaatg gcgcgtgtaa tgacatgttg atttcattaa atgtcagcca
    13861 taacgcaact ttatgttggt agcgggtaaa ggccgtgcgt gcgtaatgct cgaagtgatc
    13921 gatgaccgcc cgattagccc aaccgccgta gtttttcacc agcccatatg gcatttcgta
    13981 atgggataac gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgctc
    14041 gtaaaatgct aaccccgctt cattcggttc ggcttcatcg ccctgaggaa aaattcgcgc
    14101 ccaggcaatg gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc
    14161 cgggtaacgg tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg
    14221 cggttccatt tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc
    14281 atcttcctgc caggcgcctt ccatctgatt ggcagctgtt gcaccacccc aaagaaatgt
    14341 ttctggaaat gctttcataa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg
    14401 ctcaccagcg tttatctgcg ccgtgccgtg gggtaatacg tccgtaaaat catcgctatt
    14461 actgattaat accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa
    14521 agaaatcagc cgatcgcctg tattgacctt gtcaccccac gttgacgtga gcggaaaaga
    14581 atttgccgtc cagttttacg gtgtcgatac cgacatgaat caggatctcc acgccatcat
    14641 ctgactcaat gccgatggca tgtaatgtgg cgaacaacga agcaattcga cccgccaccg
    14701 gagaacgcac ttcaccaaca gagggcagaa tggcaatacc tttacccaac aggccgctgg
    14761 caaacgtggt atcagcgacg tgaatgagcg gcacaatctc tcccgtcatc ggtgaacaga
    14821 taccgccctg ctcaggtgta ataacctctg gtgttttctc ttgctgggca acttgcgctg
    14881 actgactctt agcggtgatg aaatgaaaca tcaccgtacc gacaaatgcg caaccgatgg
    14941 caatgacacc gccaataacg ctggcccata cggtgaaatc aatccccgtt gacgggatgg
    15001 tttgcatgaa ggtgaaaata cctggcaaac caaaggagta aactttcgtt tgcgcgtagc
    15061 caatgatggt ggcccccaat gccccactta tgcaggcgat aacgaagggg tacttacgcg
    15121 gcagattgac gccatatacc gctggttcgg tgataccaaa caaacctgtc aacgccgctg
    15181 atcccgccac cacttttttc tgtgcatcgc gttcgcagag gaagacgccg agcgccgccc
    15241 cgacttgcgc cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg
    15301 tgaagttatt gataattaaa ggtatcaaac cccaatgcag accgaacatc acaaagattt
    15361 gccagaagcc gcccattacc gcgcctgcaa atgcaggaac cgcctgataa agccagagat
    15421 aaccggcagc aatcagttca cttatccagg ttgatagcgg ccccaccagc agaaatgtga
    15481 tgggcgtgat gaccatcaga catagcaagg gtgtgaagaa atttttgatt gccgaaggta
    15541 accacgcatt aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa
    15601 taaccgatga cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccagcg
    15661 catccacttt ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccgc
    15721 caatcaccat ggcagtaaat ggattgccgc cgaagcgttt ccctgcggtg tagcccagga
    15781 ttatcgggaa gaaccaaaac aaggcatcac tggcgctaaa taaaattaaa taagtaccac
    15841 tttgttcggt cgcccactga aaagtgagtg ccagagccaa catacctttc aggatcccgg
    15901 ttgccgccat caaaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt
    15961 ttaacagatt atctttatca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg
    16021 ccacgctgtt aaccgccagg aagacatcgg ccacatggtt acctatgaca acctgaaact
    16081 ggccaccgct ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg
    16141 ctttgctttc atcctttaac ttaaaacgta atcgcgttgc acaatgcatc agactcacaa
    16201 tgttatctgc gcccccgact cctgcgacta ttgttctggc taactccgtc ataacttgcc
    16261 ctctaccgct ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat
    16321 caagcaattt ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ttcagtaata
    16381 atatttcagt gttctttgcg cacgcgctct atatttatgg ctaaaaacat aatctctgca
    16441 ggtgagattt tacgttgata ctgcaaacca ataaaaatgg cgatccgctc cgcacattgc
    16501 catgcttgcg ggtaattttg ctttactgct tgttgtaatg attcatcact atcgttaatc
    16561 gatgcatgtt caagaatacg ccaggataaa aatttcagat gcgtaaccag tcgctgataa
    16621 ctcaagcttt cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttct
    16681 cgcattaatt gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc
    16741 agatgcatgg caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca
    16801 ataatggtta acgcttcttc ccctagctgg aactctttcg ggtaaagtcg ctggatatcc
    16861 cacagcagcg ggttgggcag tagcacgttt tgctgaaagc gtttaatcgc aaactggcaa
    16921 tggtcagtta gcgagatata aatactgtcc tgcaactttc ccagacgctc ctgcgccaga
    16981 gagataatac gatcacaggt tgccatcacc tcaagaggca tatgacttaa gagttcgctt
    17041 aatcgcccgt tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta
    17101 attctttcgc ctgggcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc
    17161 tgttgatcat caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc
    17221 atagcaagga ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg
    17281 tcaggttttg cctgcgaatg cagtaacaat ccagtcatta attaatggtt tttataacga
    17341 acatccaggt tcgaaaatta atttagttgc gtgcagagaa aatagcaatg ctctatttat
    17401 aaaagtatga ccatgctcgc agttattaat tttgtgtaat tttaggattt tataaagtta
    17461 tatataacaa atcccaataa ttgaattatt gggatttact tggtgaatta tttatcgcta
    17521 tctttttctg ccagcagttt atcgagctcg tcaccaccga catgacggaa atcctgcccc
    17581 ttcacgtagt agaagataaa ctcacaaata ttctggcagc ggtcgccaat acgttcgata
    17641 gaacgcgcac agaataacgc ggtaagtacg ctcggaatgg tacgcgaatc ttccatcatg
    17701 taggtcatca gttgacgaac aataccttcg tactcctgat cgactttttt atcttcacga
    17761 taaatacgta ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc
    17821 atctggatgg tatgacggcc cagcgactcc agacttacca gcaatggctg atgctgctgg
    17881 gagaatttct ccagtgcagt acggcagatt ttgtccgcca cgtcgccaat acgctccagc
    17941 tcggcaatgg ttttactgat caccataacc agacgcaggt cgctcgccgt cggctgacgt
    18001 ttggcgataa tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt gacgttcttg
    18061 tcgccttcga tgacgcgctt cgccagatcg ctgtcctggt tatgcatcgc ggtgatcgca
    18121 tcagaaagct gctgctccac catgccgccc atggtcatca cctgcgtgcg gatgctttcc
    18181 agttcggcgt tgaactggcc ggaaatatgt ttattaagat tgagactgtc cataacgcac
    18241 tcctgaatca accgtaacga ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg
    18301 tgaacagatc gtccgtgttg ctgaactcaa tcaattcgcc caggtacata aacgccgtgt
    18361 ggtcggaaca acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct
    18421 gcttcagttc ggtgatcagc tcttcaatac gcccggtgga gatggggtcg agcgccgaac
    18481 acggttcgtc gagcagcagt acttccgggc gaatggcgat accacgcgca atgcacagac
    18541 gctgttgctg accaccagag agagagtaac cgctctggtg caatttatct ttggtttcgt
    18601 tccacaatgc ggctttggtc aatgcccact gcacgcgctc gtccatgtca gcacgggaga
    18661 gcttctcaaa cagacgcacg ccaaaagcga tgttgtcgta gatggacatc gggaacggcg
    18721 tcggtttctg gaacaccata cccactttcg cacgcagcag ggcgatatcc tgagagttgg
    18781 tcaggatgtt gtcgccatcg agcagaattt caccttccgc acgctgttcc gggtacagtt
    18841 caaacatttt gttgaaggta cgcagcagcg ttgatttacc gcagccggac ggcccgataa
    18901 acgccgtaac ctggtttttg gcgatatcca ggttgatgtt tttcagggca tggaatttgc
    18961 cgtagtagaa gttcaaatta cgaacctgaa ttttactcgg ggcagtttca accatactca
    19021 ttgcaatctt ttcctcatcg tgcctgatgc acttcgcgaa tcaggcttac cgtaggccgg
    19081 ataaggcgtt tacgccgcat ccagcaaagt gttaaccgtg tttattcttc gcaaaaacaa
    19141 cgcgcgccag aatgttcagc agcagtacgc acagggtaat gatcaacacc ccggcccagg
    19201 ccagttgctg ccattccgca aacgggctca tcgcaaactt aaagatcgtc accggcaggt
    19261 tggcgatcgg ctgcatcatg tccgtgctcc agaactggtt ggagagcgca gtaaacagca
    19321 gcggtgcggt ttcaccagca atacgggcaa tcgccagcag gataccggtc atgatcccgg
    19381 acaccgacgc tttcagcgtg atcgaggcga tcatcttccg cttcggcgta cccagcgcat
    19441 aagccgcttc acgcaggctg tccggcacca gtttcagcat gttctcggtg gtgcggataa
    19501 caatcggcac ctgcaacagc gccagcgcaa tcacgcccgc ccagccggag aagtgctcca
    19561 tctgcgccac cacaatggtg taaacaaaca gaccaaccac aatcgacggc gcagagagca
    19621 gaatgtcgtt aatgaagcga atcacttctg ccagccagga tttacgacca tattcagcca
    19681 ggtaaatccc cgccataatg cccagcggcg taccgaatac ggtagcccac aaaattaaca
    19741 ggccgctacc cgccagggcg ttcgccagac caccgccttc cgtattgggt ggtggcgtca
    19801 tttcggtgaa cagcgccagc gacataccgt cgataccgcg agtgatggtg gacattaaaa
    19861 tccagatcag ccagaacaga ccgaaggcca tcgtcgccat tgagagcgtc agcgcaatac
    19921 ggtttttgag gcggcgacgc gcctgcattt tgcggcggga ttcagccagt gccgcagtgg
    19981 tttgcatttc aaccatagcc attagcgtgc cccctcattc ttagccaggc gcataatcat
    20041 aaacttcgat gcggcgagga cgatgaaggt aatcacaaac aggatcaggc ccagttccat
    20101 cagtgcagcg acgtgcagac cggattccgc ttccgcaaat tcgttggcca gcgcagaggt
    20161 gatactgttg cccggcatat acagcgaggc gctgtcgagt tggtaggtgt taccgatgat
    20221 aaaggtcacc gccatggttt caccgagcgc gcgccccagc cccaacataa tgccgccgat
    20281 aacaccattt ttggtgaacg gaagaacgat acgccagata acttcccagg tggtgcagcc
    20341 aataccgtag gccgactctt tcatcatcac cggggtttgt tcgaatacat cgcgcattac
    20401 cgctgcaatg tacggaataa tcatgatggc gaggatcacg cctgccgcga ggataccgat
    20461 accaaaggcc gggccggaga acagcgcgcc gacaatcggg atattcgaca tgatattgcc
    20521 gaccggctcc tggaagtaaa cggcgaacag cggcgcaaag ataaacaggc cccacatgcc
    20581 gtaaacgata cttggaatgg cagccagcag ctcaatggcg atacccagcg ggcgtttcaa
    20641 ccagccaggc gcaagctcgg tcaggaacag ggcgatacca aaactcaccg ggacggcgat
    20701 cagcagcgcg ataaacgagg tcaccagcgt accgtagatc ggcaccagcg ccccgtagat
    20761 atcgttcggc gcatcccact ctttggtcca caggaaagcc agaccaaatt tctgaatgct
    20821 cggccaggat gagatgatca gagagacaat aatgccaccc aacatcaata gcacaatcag
    20881 cgccgccagt tttaccagca cgctgaaaat tatgtcgccc tttttacccg gtgggttaaa
    20941 agcaggcttg gttgcagcca taagttactc ttcagttaaa cgcgtttaca aagtcatttt
    21001 gtaatgccgg atgcggcgta aaacaccgta cccggcctgg agttttatta gtacagcggc
    21061 ttgccgctac tgtctttaat attggtcttc cacgcagcgc gaacctgttc aactacgcta
    21121 tccggcaggc tggcgtaatc caggtcgttc gcctgtttag ccccggtttt gtatgcccag
    21181 tcgaagaact tcaacacttc ggtgccttgt tctggtttct tctgatcttt atggatcaga
    21241 atgaacgtgg tagaggtaat aggccatgca tcttcgcctt tctggttggt cagatcctga
    21301 gcgaatgttt tgctccagtc tgcgcctttt gctgcattag cgaagttttc ttcggtcgga
    21361 ctcaccggtt tgccatcagc ggagatcagt ttggtgtacg ccaggttgtt ctgcttcgcg
    21421 taagcatatt caacgtaacc aattgcaccc ggcagacgct gaacgaacgc ggcgataccg
    21481 tcgttacctt taccgcccag accgatcggc cattttacgg tagagccagt accaacgttg
    21541 tttttccact cttcgttcac tttcgccagg tagctggtga agacgaagga agtcccggag
    21601 ccatctgcgc ggcgtactac agcaatgttc tgtgaaggca gtttcagacc cggattcagt
    21661 ttggcgatgg cttcatcatc ccacttcttg attttgccca ggtagatgtc gccgagggtt
    21721 ttaccatcca gcaccagttc gccagacttc agacctggaa tattaaccgc cagcaccacg
    21781 ccgccaatca cggtcgggaa ctggaacagg ccttcctgag ccagtttttc gtcagacagc
    21841 ggcgcgtcag aggcaccgaa atcaacggtg ttagcgataa tctgttttac gccaccggaa
    21901 gaaccgatac cctggtagtt aactttatta ccggtttctt tctggtaagt gtcagcccat
    21961 ttggcataca ccggcgcagg gaaggttgca cctgcacctg tcaggcttgc ttctgcaaac
    22021 acagagaaag cgctcatcga taaggtcgcg gcgacaacag ttgcgacggt ggtacgcata
    22081 actttcataa tgtctcctgg gaggattcat aaagcattgt ttgttggcta cgagaagcaa
    22141 aataggacaa acaggtgaca gttatatgta aggtttatga cagttttatg acagagagag
    22201 aatgtc
//
LOCUS       DAZFRG010000061        21828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFRG010000061 DAZFRG010000000
VERSION     DAZFRG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.061"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..656
                     /gene="zapC"
                     /locus_tag="ACHKUC_001809"
     CDS             114..656
                     /gene="zapC"
                     /locus_tag="ACHKUC_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO6618073.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(653..1762)
                     /gene="ycbX"
                     /locus_tag="ACHKUC_001810"
     CDS             complement(653..1762)
                     /gene="ycbX"
                     /locus_tag="ACHKUC_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO6618074.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            1860..3968
                     /gene="rlmKL"
                     /locus_tag="ACHKUC_001811"
     CDS             1860..3968
                     /gene="rlmKL"
                     /locus_tag="ACHKUC_001811"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO6618075.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLVNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATILVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            3980..5887
                     /gene="uup"
                     /locus_tag="ACHKUC_001812"
     CDS             3980..5887
                     /gene="uup"
                     /locus_tag="ACHKUC_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6618076.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDVIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQELLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6017..7270
                     /gene="pqiA"
                     /locus_tag="ACHKUC_001813"
     CDS             6017..7270
                     /gene="pqiA"
                     /locus_tag="ACHKUC_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO6618077.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7275..8915
                     /gene="pqiB"
                     /locus_tag="ACHKUC_001814"
     CDS             7275..8915
                     /gene="pqiB"
                     /locus_tag="ACHKUC_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO6618078.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKGKKDPEPKRAKQ"
     gene            8912..9475
                     /gene="pqiC"
                     /locus_tag="ACHKUC_001815"
     CDS             8912..9475
                     /gene="pqiC"
                     /locus_tag="ACHKUC_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO6618079.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9731..9898
                     /gene="rmf"
                     /locus_tag="ACHKUC_001816"
     CDS             9731..9898
                     /gene="rmf"
                     /locus_tag="ACHKUC_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO6618080.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(9968..10486)
                     /gene="fabA"
                     /locus_tag="ACHKUC_001817"
     CDS             complement(9968..10486)
                     /gene="fabA"
                     /locus_tag="ACHKUC_001817"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO6618081.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(10555..12315)
                     /gene="ycbZ"
                     /locus_tag="ACHKUC_001818"
     CDS             complement(10555..12315)
                     /gene="ycbZ"
                     /locus_tag="ACHKUC_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6618082.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDDFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12501..12953
                     /gene="matP"
                     /locus_tag="ACHKUC_001819"
     CDS             12501..12953
                     /gene="matP"
                     /locus_tag="ACHKUC_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO6618083.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13031..14071)
                     /gene="ompA"
                     /locus_tag="ACHKUC_001820"
     CDS             complement(13031..14071)
                     /gene="ompA"
                     /locus_tag="ACHKUC_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO6618084.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNFDGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14427..14936)
                     /gene="sulA"
                     /locus_tag="ACHKUC_001821"
     CDS             complement(14427..14936)
                     /gene="sulA"
                     /locus_tag="ACHKUC_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO6618085.1"
                     /translation="MYTSGYAHRSSSFSSTASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15155..15784
                     /gene="sxy"
                     /locus_tag="ACHKUC_001822"
     CDS             15155..15784
                     /gene="sxy"
                     /locus_tag="ACHKUC_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO6618086.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAPYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNIRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15747..17909)
                     /gene="yccS"
                     /locus_tag="ACHKUC_001823"
     CDS             complement(15747..17909)
                     /gene="yccS"
                     /locus_tag="ACHKUC_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO6618087.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(17919..18365)
                     /gene="yccF"
                     /locus_tag="ACHKUC_001824"
     CDS             complement(17919..18365)
                     /gene="yccF"
                     /locus_tag="ACHKUC_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO6618088.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            18488..20542
                     /gene="helD"
                     /locus_tag="ACHKUC_001825"
     CDS             18488..20542
                     /gene="helD"
                     /locus_tag="ACHKUC_001825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO6618089.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMHERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20574..21032)
                     /gene="mgsA"
                     /locus_tag="ACHKUC_001826"
     CDS             complement(20574..21032)
                     /gene="mgsA"
                     /locus_tag="ACHKUC_001826"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO6618090.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21128..21790)
                     /gene="yccT"
                     /locus_tag="ACHKUC_001827"
     CDS             complement(21128..21790)
                     /gene="yccT"
                     /locus_tag="ACHKUC_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO6618091.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         5573 a         5249 c         5786 g         5220 t
ORIGIN      
        1 ttatattcat cgctgttgct tatttagaca ttttgtactt ttattgttga ggttattaag
       61 cgaagcgaca atggattgtg tcgctgcggt tttataaggt tggggagtgg cagatgcgaa
      121 ttaaaccaga cgataactgg cgttggtatt acgatgaaga gcacgatcga atgatgctcg
      181 atttagccaa tggtatgcta tttcgctcac gttttgcgcg caaaatgttg actccagatg
      241 ccttttctcc cgcaggtttt tgcgttgatg acgccgcgct ctatttctct tttgaagaaa
      301 agtgccgcga ttttaattta tctaaagaac aaaaagccga actggtgctg aatgcactgg
      361 tagcaattcg ttatctcaaa ccgcaaatgc cgaaaagctg gcattttgta tctcatggtg
      421 aaatgtggat gcccatgccg ggcgatgcgg cctacgtttg gttgagcgac actcatgagc
      481 aagtgaattt actggtcgtt gaatctggcg agaatgccgc gctatgtcta ctggcacaac
      541 cctgcgttgt tattgcgggt cgcgcgatgc agttgggcga cgccattaaa atcatgaacg
      601 acaggctgaa accgcaggtt aatgttgaca gcttcagcct cgaacaggca gtctaacgcg
      661 ccagcttaag cgcagtcttc ggtacacaac tacagcaaag aatagtgcca tcatcgccta
      721 ttgctgattt ttttagcggc gtgacttcgc cttctaaaag ctgaacacgg caacttccac
      781 aaatgcccgc gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca
      841 gcacctgttg gttatttcca cgaaatgcct gtccctgcca gtcaatatct acgtttgcgt
      901 ccggttgttg cgtgatgttg gcagtatcat cagcggcacc tgcgccgtaa attttagccg
      961 gagccgttgc cagaatttcc acctcgtcgc caacgcgaat cacgccgcta ttacgggcaa
     1021 ttaaattctg accaaaatcg acatcgccgt tatcctgggc agtacggaaa gattgcagcg
     1081 tttttaatgg ttcgcctgcc ggatgttttt gccctttttc tgggctgacg gtggtgaaaa
     1141 tacagcggct acaaggctta accacatcaa acacgacatc accaatgcga atcactttcc
     1201 agctatcttc ttcccaggcc gaagcaccgg aaaccaccag attggggcgg aattgctcca
     1261 tttttacact ggccggacaa cgttgttgga gatcacgtaa cgaggcctcg ttagcaagaa
     1321 ggtaaggata gccatcagca aatgacaggg gtacagtgtt gtggcgtttc acgcgccggg
     1381 tcatttgtgg ccccacccag cgtaattgca cttcgcggga gaaaaatcca cttagccatt
     1441 tgttgatcgc gtctggcgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg
     1501 cgtcttgtgt ggcgaaatca gcaaaacgaa catatgcact actgccatct ggtgcggtga
     1561 gatgcaagcc atcatgcacg ggtgaaggag taaaccgtac cattagcgga aactggcgag
     1621 cggtaataaa cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccagaccac
     1681 tgacatctgc cagggtatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa
     1741 aaagccgggt taatgtcgcc acggtgcggt cctcaaatga aaataagccc tcaactttat
     1801 gacatgacgc gcttattagc tataatgcgc aacaattttc ttagagtaaa agtgacaata
     1861 tgaattctct gtttgccagt acggcccgtg ggctggaaga gctgttaaaa actgaactgg
     1921 aaaacctggg ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc aagggcgaca
     1981 cacggcttgt ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt atgttgccgc
     2041 tgggcgagtg taaggtttac agtgatttag acctctatct cggcgttcag gcgatcaact
     2101 ggacagagat gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt ttgaatgaca
     2161 ccatacgcaa cagtcagtac ggtgcgatga aagtgaaaga cgcgatcgtc gatgctttca
     2221 cgcggaaaaa tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc cgcgttaacg
     2281 tctggctgca taaagaaacc gccagtatcg cccttgatct cagtggtgat ggtttacatc
     2341 tgcgtggcta tcgcgatcgt gctggcattg cgccgatcaa agaaaccctg gcagccgcta
     2401 ttgtgatgcg atccggctgg cagccaggaa caccgctgct cgatccaatg tgtggttcag
     2461 gtacgttgct gattgaagca gcgatgctgg cgaccgaccg cgcaccgggc ttgcaccgtg
     2521 ggcgttgggg ctttagcggc tggacgcagc atgatgaagc tatctggcag gaagtgaaag
     2581 cggaagcgca aactcgtgcc cgtaaaggcc tggcggagta tagctcccat ttctacggtt
     2641 cggacagtga cgcacgggtg attcaacgtg cgcgcactaa cgcccgtctt gcggggattg
     2701 gtgaactgat cacctttgag gtgaacgatg tcgcgcaact ggtcaatccg ctgccgaaag
     2761 ggccgtacgg tacagtgttg agcaacccgc catacggtga acgtctggac agcgaaccgg
     2821 cgctgattgc gctgcatagt ctgctgggcc gaatcatgaa aaaccagttc ggtggctgga
     2881 atctctcttt gtttagtgcc tcgccggatc tgctaagctg cctgcagctg cgtgcagaca
     2941 aacagtacaa ggcgaaaaac ggcccgctgg actgcgtaca gaaaaactac catgttgccg
     3001 aaagcacacc agacagcaaa ccggtaatgg cagcggaaga ctacgccaac cgtctgcgta
     3061 agaacctcaa aaaattcgag aagtgggctc gccaggaagg aattgaatgt taccgcctgt
     3121 atgacgccga tctgccggaa tataacgttg ccgttgaccg ttatgccgac tgggtggtgg
     3181 tgcaggagta tgcgccgcca aaaactattg atgcgcacaa agcgcgtcag cgtctgttcg
     3241 atattatcgc tgcaaccatt ttggtactgg ggattgcgcc aaacaaactg gtgctaaaaa
     3301 cccgtgaacg ccagaagggc aaaaatcaat accagaaact gggcgagaag ggcgagtttc
     3361 tggaagttac cgaatataac gctcacttgt gggtgaacct gacggattac ctcgataccg
     3421 gtctgttcct cgatcaccgt atcgcccgtc gtatgctcgg tcagatgagc aaaggcaaag
     3481 atttcctcaa cctgttctct tataccggca gcgccaccgt gcacgcggga ttaggcggtg
     3541 cacgcagcac taccaccgtg gatatgtcgc gtacttatct ggagtgggca gaacgcaacc
     3601 tgcgtctgaa tggcctaacc gggcgtgcgc atcgcctgat tcaggccgat tgcctggcgt
     3661 ggctgcgtga ggctaatgaa cagttcgatc tgatatttat cgatccacca accttctcta
     3721 actcgaaacg aatggaagat gcgtttgatg ttcagcgcga tcatctggcg ctgatgaaag
     3781 atttgaaacg tctgctgcgt gctggtggga cgatcatgtt ctcgaacaac aaacgtggct
     3841 tccgtatgga tcttgacgga ctggcaaaac tgggactgaa agcacaagaa attacgcaaa
     3901 aaacgctctc ccaggatttc gcccgtaacc gccagatcca caactgctgg ctgattaccg
     3961 cagcctgaaa ggaatagtaa tgtcattaat cagtatgcat ggcgcatggc tgtcgttcag
     4021 cgacgcgccg cttctcgata acgcagaact gcatatcgaa gataacgaac gtgtttgtct
     4081 ggtggggcgt aacggcgcag gcaaatcgac gttaatgaag atcctcaacc gtgaacaggg
     4141 gctggatgac ggtcgcatta tttatgagca agatgtgatt gtagctcgtc tgcaacagga
     4201 tccgccgcgt aacgttgagg gtagcgttta tgatttcgtt gccgaaggta ttgaagaaca
     4261 agcggaatac ctgaaacgct atcacgatat ttcgcgcctg gtgatgaacg acccgagcga
     4321 gaaaaatctc aacgaactgg cgaaggttca ggaactgctg gatcaccaca acctgtggca
     4381 gctggaaaac cgtatcaacg aagtgctggc gcaactgggg ttagatccta atgttgcgct
     4441 gtcgtcgctt tccggcggtt ggttgcgtaa agcggcatta ggacgcgcgc tggtgagcaa
     4501 tccgcgcgta ttgctgctcg acgaaccgac taaccacctg gatatcgaaa ccatcgactg
     4561 gctggaagga ttccttaaaa ccttcaatgg gacgattatt ttcatctccc acgaccgttc
     4621 gtttatccgc aatatggcga cgcgtattgt tgatctcgat cgcggcaaac ttgtgaccta
     4681 tccagggaat tacgaccagt acctgctgga aaaagaagaa gccctgcgcg tggaagaact
     4741 acaaaatgcc gagttcgatc gcaaactggc gcaggaagaa gtgtggatcc gtcaggggat
     4801 caaagcacgc cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcaa tgcgtcgcga
     4861 gcgtggcgaa cgtcgcgaag tgatgggtac cgcaaaaatg caggtggaag aagccagccg
     4921 ctccggtaag atcgttttcg aaatggaaga cgtttgctac caggttgacg gtaagcaact
     4981 ggtgaaagat ttttctgccc aggttctacg cggcgacaaa attgccctga ttggtccgaa
     5041 tgggtgcggc aaaaccacgc tgctgaaact aatgctcggt cagcttcaag cagacagcgg
     5101 gcgtattcat gttggcacca aactggaagt ggcttatttc gatcagcacc gcgcggaact
     5161 ggatcccgat aaaacggtga tggataacct tgctgaaggt aagcaagagg tgatggttaa
     5221 cggcaagcca cgccacgtat tgggctattt gcaggacttc ctgttccatc caaaacgggc
     5281 gatgacgccg gtacgtgcgc tttctggcgg tgagcgtaac cgcttgttgt tggcgcgttt
     5341 gttcctcaaa ccaagcaact tattgattct tgacgaaccg accaacgatc ttgatgtcga
     5401 aacgctggaa ctgctggaag aactgatcga cagctatcag ggcacggtat tgctggttag
     5461 ccacgatcgt cagtttgtcg ataacaccgt tacagaatgc tggatcttcg aaggcggcgg
     5521 taaaattggt cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag aacagtatgt
     5581 ggcgctcaaa cagcctgcgg tgaaaaaaag cgaagaagcc gctgcgccaa aagcagaaac
     5641 tgtaaaacgc agcagtagca aactaagcta taaattgcag cgcgaactgg agcagctacc
     5701 gcaattgctt gaagatctgg aggcgaagct ggaagcttta cagacgcaag tggcggatgc
     5761 ttccttcttc agtcagcccc atgagcagac acaaaaagtg ctcgccgata tggctgctgc
     5821 agagcaggag ctggagcaag cctttgaacg ctgggagtat cttgaagcgt taaaaaatgg
     5881 tggctgatcg caaaatagtc gatttaaaca gagacagctg acaacggtaa aattgcctga
     5941 tgaactgggt ttatcaggca tgaaaaccaa cactttgtta gtcatctgca aaatagcgca
     6001 tcattaagga gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc tgtgctcgca
     6061 gtgtgacatg ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg catgtccccg
     6121 gtgtggcaca acgttaaccg tggcgtggga tgcccctcgg cagcgtccca ccgcctatgc
     6181 gttggctgca ctgttcatgc tgttgctgtc caacttgttt ccttttgtga atatgaacgt
     6241 tgcaggagtt accagtgaaa ttacattact ggaaattccc ggcgtgcttt tttctgagga
     6301 ctacgccagc ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg cgttttgtct
     6361 gataaccatt ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa aagagcaact
     6421 ggcacgggtg ctttttcaac tcaaaacctg gggaatggcg gagattttcc tcgcgggtgt
     6481 gctggtcagt ttcgttaaac tgatggctta cggcagcatt ggggtaggca gcagctttct
     6541 cccctggtgt ttattttgtg tcctgcaact gcgcgctttt cagtgcgttg atcgtcgctg
     6601 gttatgggac gacatcgccc cgatgccaga actgcgccag ccgctaaaac caggtgtcac
     6661 ggggatacgt caggggctgc gttcgtgctc ctgttgtacg gcaatccttc ctgctgatga
     6721 acccgtgtgc ccgcgttgta gtaccaaagg gtacgttcgg cgtagaaaca gcctgcagtg
     6781 gacactcgcg ctgcttgtaa cgtccatcat gctgtatctt ccggctaata ttttgcccat
     6841 catggtgacg gatttattag gctcgaagat gccgtcgacg attctcgctg gggtcattct
     6901 gttatggagc gaaggctctt atcccgtcgc tgcggtgatt tttctggcca gtattatggt
     6961 gccaacgtta aagatgatcg ccattgcgtg gctgtgttgg gatgccaaag gacacggcaa
     7021 gcgcgacagt gaaagaatgc atttgattta tgaagttgtt gagtttgtag gccgctggtc
     7081 gatgattgac gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg gaggtttaat
     7141 gagtatttat ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa tgacaatgtt
     7201 ttctgctatg acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat cagagcatga
     7261 ggagtcctga cagtatggaa tctaataatg gggaagccaa aatccagaaa gtgaagaact
     7321 ggtctcccgt gtggatattt cctatcgtca cggcgctcat tggggcctgg gttctttttt
     7381 atcattacag ccatcaggga ccggaagtga ccctgatcac cgcgaatgcg gaaggaattg
     7441 aaggtggcaa aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt gaaagcgcca
     7501 cactggctga tgatttgacg cacgttgaaa tcaaagcgcg gctgaattcc ggtatggaaa
     7561 aattgctgca taaagatacc gtcttttggg tggtgaaacc gcagattggt cgagaaggga
     7621 ttagcggcct gggaacgcta ctgtctgggg tttatatcga actgcagcca ggcgcgaaag
     7681 gcagcaaaat ggataaatac gatttgttgg actcgccacc gttggccccg cctgatgcga
     7741 aaggtatccg tgtgcttctc gatagcaaaa aagccgggca gctctcgcca ggagatccgg
     7801 tgctgttccg tggctatcgg gtaggttcgg ttgaaaccag cactttcgat acgcaaaaac
     7861 gcaatatcag ctatcaactg ttcatcaatg caccttatga ccgactggtg accagcaatg
     7921 ttcgtttctg gaaagatagt ggcattgcgg ttgatctgac gtcagcggga atgcgtgtgg
     7981 agatgggctc attgacaacg ctgcttagtg gcggtgtcag ctttgatgtg ccggaaggtc
     8041 tggatttagg gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat gatcagaaga
     8101 gcattcagga ttcgttgtac accgatcaca ttgattatct gatgttcttt aaagattcgg
     8161 tacgcggtct gcaaccggga gctccggtag aattccgggg tattcgcctg ggtaccgtaa
     8221 gcaaagtgcc attctttgcg ccgaatatgc gtcagacatt taacgatgat taccgtattc
     8281 cggtactgat tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa aatgcggatg
     8341 ttgttgagca ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg aaaaccggaa
     8401 acctggtcac tggcgcactg tatgttgatc tcgatttcta tccaaatacg cctgcaataa
     8461 ccggtattcg tgaatttaat ggttatcaga ttatcccgac cgtcagcggc ggcctggcgc
     8521 aaatccagca acgactgatg gaagcgttgg ataagatcaa caaactgcca ctgaatccga
     8581 tgattgaaca ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa aacctgcaaa
     8641 cgacgctgga tagcatgaac aagatccttg ccagccagtc gatgcagcag ttaccgacgg
     8701 atatgcagtc aacgttgcgt gaattgaatc gcagcatgca gggcttccag cctggttccg
     8761 cagcctacaa caagatggtg gcggatatgc agcgccttga tcaggtgttg cgagaactgc
     8821 aaccggtgct gaaaacgctc aacgagaaga gtaacgcgct ggtatttgaa gcgaagggca
     8881 aaaaagatcc agagccgaag agggcgaaac aatgaaaaag tggctagtga cgattgcggc
     8941 actgtggctg gccggatgca gctccggcga aattaataaa aactattacc agttacctgt
     9001 ggtgcagagc ggtacacaaa gcaccgccag ccagggtaat cgcctgttat gggttgagca
     9061 ggtcgctgtt cctgactatc tggcggggaa tggtgtggtt tatcaaacca gtgatgtgaa
     9121 gtatgtgatt gccaacaaca acctgtgggc cagcccgttg gatcaacagt tgcgcaacac
     9181 cctggttgcc aacctgagta cgcaactacc cggctgggtg gttgcctccc agcctctggg
     9241 aagcgcccag gacacgctca atgttaccgt cacggagttt aacggtcgct atgatggtaa
     9301 ggtcattgtc agtggtgagt ggctgttgaa ccaccaggga caactgatca aacgtccgtt
     9361 ccgtctggaa ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag tgctggccag
     9421 tgtctggagt caggaagccg cttctattgc gcaagagata aagcgtctac cttaatcata
     9481 aagatttgta aatataaccg tctccggtat gttgcctgag gcggtttttt tgtttctaac
     9541 gtgcggaaaa agttgttcct cttcacattt tttgtacaac cgacatgccc gtgtagctca
     9601 caaatatgac agtggcgtga attttgcgca ttgacggcag ttatgattcg cggtattgct
     9661 taactgtgat tgcatattta gtaatcactg ttttcttttc caccagaaac cagtatgagg
     9721 gaaacgaggc atgaagagac aaaaacgaga tcgcctggaa cgggcacatc aacgtggtta
     9781 tcaggccggc atcgccggac gctcaaaaga aatgtgtccc tatcagacgc tgaatcaaag
     9841 gtcacaatgg ctgggaggct ggcgagaagc catggcggac agggtagtaa tggcctgatt
     9901 ctgtctcttt aaaaagaaac ctccgcattg cggaggtttc gcctttatat actctgtctg
     9961 attaaaatca gaatgcagac gtatcctgga acagacctac tttcaggtcg ttggcggtat
    10021 agatcaaacg accatcaacc agcacttcac catcagccag gcccataatc agacgacggt
    10081 taactatgcg tttaaagtga atacgatagg tcactttttt cgccgtcggc agcacctggc
    10141 ctgtgaattt cacttcacct acgcctagcg cgcgaccttt accttcgccg cccagccagc
    10201 cgaggtagaa ccctaccagc tgccacattg cgtccaggcc caggcatccc ggcataaccg
    10261 gatcgccgat aaagtggcat ccgaagaacc acagatccgg attgatatcc agctctgctt
    10321 caacataccc tttgtcgaag ttaccacccg tttcggtcat tttgaccaca cggtccatca
    10381 tcagcatgtt tggtgctggc aattgcgggc ctttagcacc aaacagttca ccgcgaccag
    10441 aggcaagaag gtcttctttt gtataggatt cgcgtttatc taccatgttc tctgtaagcc
    10501 ttattttatt gaagcacgca ggatagctaa cacgtgtacg ctgaacaagt ccgatcagtt
    10561 cggaataaac cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc cttcctgttg
    10621 cgatgcttgt gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc cttcgccatc
    10681 ccacaccaga tttaataata acggcagtgc gtcagtcaca tcgtctactg cccagatggt
    10741 gaatttgtct tcttctaccg ctttcaccag ttcactgtga agacttaaat ggcgaacgtt
    10801 agctgtgggg ataatgacac cttgtttccc cgttaactca cgttgctggc aaatagcaaa
    10861 gaagccttcg attttctcat ttaaaccacc gactggctgg gcgcgaccga actgatcgac
    10921 tgaacctgtg atagcgatac tctgattcac cggcacatcg gcgagggcgc ttatcagggc
    10981 gcagagttca gccatcgagg cactatcgcc atccacttca ctgtatgact gctcaaatgt
    11041 cagcgatgct gagaagggga tctgttgctc aagctgtagt tccgacatca ggaacgcttg
    11101 catgatcatc atccctttcg catggatatt gccgccaagc tccgctttgc gttcgatgtc
    11161 ggtgaattca ccatcgccaa tatgcacaac gcagctaatg cgagaaggtt cgccaaaagc
    11221 gcgtggatga cccggaaatt caatgaccga aagggcgtta atttgcccga tgcgttcgcc
    11281 ttcggtttct atcaggattt gctcctgaag gatctcatcc tgcatgcgtt cagcgaggaa
    11341 accttcgcgc cattcacgct gctgtagcat taagtttagc tgctcgccgg agaaggtgtc
    11401 gccatcacac agggaggcga cctcttgaca ctggcggagg atccactgcg ggctaagcgg
    11461 aagtgtttct tgttcaccag tgtagcgtgc tgcttcgcgg ataagtaccg gccaggcatc
    11521 cgctcccggt gctggtaagt gattatgtct ggcggtaaat gtcacccagc gacaccactg
    11581 gcttactgac tccgcatcga caatctgcag agtatcttca aattcgctat aaatagcctg
    11641 ctctgaaagc tctggctcca tctcctggaa atcagccaat gattcgcgtt cgcctaccag
    11701 aatgactttc agcttcaatg gcatcgaagg cacagagacg gggagagggc gcgactcatc
    11761 aaacgcaacc cagtcaaaac gctcgcggtt aacgatattt ttcagccgca tccacagcag
    11821 aggttgcgcc agcagtgtac gcaaagagat gatgagaata ccgccatttg cctgatgcac
    11881 cagaccaggc tgcagggtaa tatcgccatt aaactgacgc aggcagccaa agagttgctc
    11941 tgcttctacc cagtcggcag cgacaacttg cgttaaagtc gcaaaatcat catctgcact
    12001 cactgcgtga cgtaagcgga tggtgtggcc ggaaacctca tagtgaccgc ccaccagttg
    12061 gcctgcatcg ctttgtagcg tacgcgcggc atcagcaata agattcagat actcagactc
    12121 ttccggggcc ttcgccagca taaaagagga ggatgctcgc gtatgcagca actgctccag
    12181 cgcaaattgc agtcgcggtt gagtatcact gaataaagga tcgttttcgt caatcaaatg
    12241 tggctgagca aatatttcct gatagctatc ggtatcagga accaggtcac gccatgcaag
    12301 tttcgtaatg gtcaaagttg atgtttttta gtctgttgtc aaagccgcga ttataccgta
    12361 accggcactg cagcacacgt agaaagcaac gacaatactc ctggcatggg cgttaaagct
    12421 cacaggatgg agattctttt cttcactggc ctaaaaagct gatattctgt aaagagttac
    12481 acggtaacat tgagatcgct atgaaatatc aacaacttga aaatcttgaa agcggttgga
    12541 aatggaagta tctggtgaag aagcaccgcg aaggggagtt aatcacccgt tacatagaag
    12601 ccagtgccgc ccaggaagcc gtcgatgagt tgctctcgct ggaaaatgaa cccgtgctgg
    12661 taaatggttg gattgacaag catatgaatc cggaactggt caatcgcatg aagcagacca
    12721 ttcgggccag acggaagcgc cattttaatg cagaacacca gcatacgcgc aaaaaatcga
    12781 tcgatctgga atttatcgtc tggcaacgtc tggctggcct tgcgcagcgt cgcggtaaaa
    12841 cgctttctga aacgattgtt cagctgattg aagatgcgga aaacaaagag aaatacgcga
    12901 ataaaatgtc ttctttgaag caggatctgc aggcattgct gggtaaggaa taactgtcaa
    12961 aagtcagttc aatttactaa aggcgaaaaa aaaaccccgc agctgcgggg tttttctacc
    13021 agacgagaac ttaagcctgc ggctgagtta caacgtcttt gatgccttta acttcgatct
    13081 ctacgcgacg atccggagcc aggcaatcga tcagtgcagc acgctgtttc acgttgtcac
    13141 aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatt ttgtctgccg
    13201 ggataccttt agagatcagg taatcaacaa cggactgagc acgacgctcg gacagagcct
    13261 ggttataagc gtcagaaccg atgcggtcag tgtaacccag aacaactacg gaaccgtctt
    13321 tcggatccag gttgctcagc tggctgtaca gctgatccag agcagcctga ccttccggtt
    13381 tcagggttgc tttgttgaag gtgaacagaa cgtcagactt cagagtgaag tgcttggtct
    13441 gtacttccgg tgccggagct ggagccggag caactactgg agctgcttcg ccctgaccga
    13501 aacggtagga aacacccagg ctcagcatgc cgttgtccgg acgagtaccg atggtgtgtg
    13561 cgtcaccgat gttgttggtc cactggtatt ccagacgggt agcgatttca ggagtgatcg
    13621 catactcaac accgccagcg aagaccggag aaacgccggt atcgtggttt ttaccatcaa
    13681 agttggattt agtgtctgca cgccatacca taccacccag acgagtgtat acgtccagat
    13741 cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct ttatatgcac
    13801 cgttttcaac gctgcctttg tatggcatac gacctaacca gtcataaccc atttcaaagc
    13861 caacatacgg gttaacctgg taaccaccaa aagcaccagc gcccagttgg ttttcatggg
    13921 ttggaccatt gttgttgatg aaaccagtgt catggtactg ggaccagccc agtttagcac
    13981 cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg
    14041 ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca tccaaaatac
    14101 gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggat ctgctcaata
    14161 ttaactctac cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac
    14221 gtagttgaaa acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa
    14281 ggtacgttta atcttttttg tcagcgacaa tttacagaag aaaatcgtgg aaaccgcttc
    14341 agacaagcct ccgcaaggaa aattagtcac gactgaaagc attggctggg cgataaaaaa
    14401 agttccagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg
    14461 gaaagttgtc tcgttgcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg
    14521 ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc
    14581 aaccaaccga tcaccacgct gtaattgccc gtgcgtaaag cgcgaaccat tgactccacg
    14641 gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc
    14701 tgaacccatt cgcgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc
    14761 tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg
    14821 cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt
    14881 ttacttgctg tggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc
    14941 aatccagccc ctgtgagtta ctgtatggat gtacagtaca tccagtgacg acaaagatca
    15001 actctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta
    15061 atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat
    15121 cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat
    15181 ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg
    15241 tagttacagc ctaaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta
    15301 tcttcgggct tgtgagcaaa gtgcaccgta ctgtgtaaaa catccgcctg tctggctgac
    15361 atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg
    15421 gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga
    15481 gaaaagcacg cgcaatattc gggaaagact gaaagatttg cccaatatgt cttttcatct
    15541 ggaagcgatt ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc
    15601 aaaaatgtgt tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt
    15661 tatgcttgaa ggggccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca
    15721 ggagcttgca gaatgggctg actctcttac gccgaaacag gagtttcctg cggaacttga
    15781 gtaatctggc gttgcagacg accaatctct ggcagtaatg caatcaataa cccaacttgt
    15841 tgtacgacca gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc
    15901 aggccagcta atgcctgatt gacgcgttct tcatcagcag gttgatgatg tagcgcgtca
    15961 tcaacatagc aaacggcgtc atcaagaaac gccagaattt caggattagt taactgctcc
    16021 cggtgagcac cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga
    16081 aacgcggctt cgcgaatttg cggggtaacg ttcggttcgc tggacatatt tgataccacc
    16141 gacgccagtt cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg
    16201 ttatcacgcc cctgatggta ttgttccagt atggcatcta gataccggca gttggcctct
    16261 gtggcgcgtt cgagcattcg cggcagattg cgaaacttcc agtcaggcca gatgtagctc
    16321 actgccgccc acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgccact
    16381 tcaaaacctt cacccagcaa gttgaaacac agtagcacca aaagtgtgat gaacatcgtt
    16441 gcatgggcgt attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc
    16501 accagctgcc cttccagtga tggcacaaac cacagcacag gaataccaat tgcgatacct
    16561 accagcgtac caataatcct taacttcagc cggtggcgcg tggcgttata gtttggctgg
    16621 cagacaaaca aacttgtcag caagatccaa tacccgtgat gcattccggt tatctgaatg
    16681 atggcgtagc cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat
    16741 tccggcgtga agtgacggct aagacgcagc cagatatcac tcaatccgtg cgggctgtca
    16801 tcagcgagct cattttcgtc attattatgg ggcagtgcct gggcctgttc tgattcaatt
    16861 gttgccagtt gggcatcaat ggcacgtaaa ttgttcagta aaaatcccag tgttttgagt
    16921 aaatcagcag gagcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa
    16981 gcgcgctcaa aatgcggatc atgttgataa ggctgacgca gcaaaataca gcgtgacagt
    17041 tgctggcacg cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg
    17101 tggcgaaaat gttcacgcaa tgtttgatat tgaatatgag aagagctggc acgctcgtga
    17161 atatcctgtg cgacaaagta ataatgcagt gtgcgacgcg ttcctcgttg accacgatcg
    17221 ccacgtaagc gggtcagcag cgagagtttc gtctgattca atgtcgccat cagctgaccg
    17281 ttggcgagag ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac
    17341 atgcgcgact tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc
    17401 tgcagcgggc ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg
    17461 gcaccggcca gaagatacat tggctgctga taccagtgct catacagtga tgttcccaac
    17521 atagtgtaaa tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga
    17581 ccgccgagca aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc
    17641 cagggaaaca gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg
    17701 aggttacgca aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc
    17761 ccaagcgtca gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt
    17821 ccacaaagcg caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt
    17881 ttgagcaaag gacttagcat aaaggccata ttagtggatt attggaaacg acgacgagca
    17941 ttggcttcgc gcgcagcttg cgctgtttct accgatacca cgcgacgacc gaccggccat
    18001 agtgcaatag cggcaatttt aaagttcgca ataccgacag gaatgccaat gatggaaata
    18061 cattgtgcga tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc
    18121 cagaaaatat tcaataccgt accgccagta tttagcagca catttttgcc agccgggttc
    18181 agttcatcga catgaatagc ttcattgcca taaggcacca gagacagttt ggtgatctcc
    18241 cagcaggatc gtgtcagtgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac
    18301 agccagccca gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta
    18361 cgcataaaac ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt
    18421 gggctggagc acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta
    18481 cagagtcatg gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga
    18541 tcgggcggtg atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat
    18601 tcctttcaat caattactgg cgattcactg taagcgcggt ctggtatggg gcgaactgga
    18661 gtttgtactg ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca
    18721 gcgtttttac catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat
    18781 tgcgtctggc gttttacgtc agcaactgga tttgattgcc acgcgcaccg gagaaaataa
    18841 atggctgacg cgtgagcaaa cctctggcgt gcagcaacaa atccgccagg ctttgtcggc
    18901 gttaccattg ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa
    18961 atgtcaggcc tggttgaagg acattgaagg cgctcggttg cagcataatc aggcgtatac
    19021 cgaagccatg cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa
    19081 tccggcgcag gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc
    19141 aggaagcgga aaaacgtcgg tgctggtggc ccgtgctggc tggttactgg cgcgtggtga
    19201 agcgtctcct gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgca
    19261 cgagcgtatt cgagaacgac tacataccga agacattacc gcacgcacgt ttcatgcgct
    19321 ggcgctgcat attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa
    19381 tgataccgct gcccgtcatg aactttttat tgctgagtgg cgcaagcaat gcagcgaaaa
    19441 gaaagcgcag gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc
    19501 agaaggtaat ttctgggatg atgaaaaatt acagcgtcgt cttgcctctc gcctcgatcg
    19561 ctgggtaagt ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc
    19621 cgaagagatt cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc
    19681 ctggaaaggt gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcaccaggc
    19741 gattgtgatt ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga
    19801 atttcaggat atctcgccgc agcgtgcagc gttgttagcg gcattacgca agcaaaatag
    19861 tcaaacgacg ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc
    19921 gcaaatgtcg cttaccacgg ctttccatga aaactttggt gaaggtgatc gctgtgattt
    19981 agacacgacc taccgtttta acagtcgtat cggtgaggtg gcaaacaggt ttattcagca
    20041 gaacccaggc cagcttaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt
    20101 cacgttattg gatgagggtc aacttgacgc tttgctggat aaactctctg gttatgccaa
    20161 accggaagag cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga
    20221 aaaagcagca acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa
    20281 agggcaacag gcggattacg tcatcatcgt tggcttgcag gaggggagtg atggttttcc
    20341 ggctgcggcg cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc
    20401 ggacgctgaa gaacggcggt taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg
    20461 ggcactgttt aacaaagaga atccctctcc ctttgtggaa atactgaaaa atctggatgt
    20521 gccggtggcg agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc
    20581 agacggtccg cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa
    20641 tgcggcgact ggataatgaa gtctgccgtt gccacgttgg tggcaaccgg aatgttccat
    20701 accgtcgcca gacgcagcaa ggctttcacg tcaggatcgt gcggaacggc atttagtggg
    20761 tcccagaaga aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg
    20821 tcacccccca ttgggccact caacatcgcg ttgacgttca tgccggttgc acgggaaatc
    20881 aagttaccgg tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgtcgt
    20941 tccacccagc tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc
    21001 cgcgcaggta aagtgcgtgt cgtcagttcc ataatgtaca tccgtagtta actttcctac
    21061 agattactgt aagcacttat cgctgcaaga gaaagaccga aaaagcctgc gcacaggcac
    21121 aaaaatctca ggaagatggt tgtttttccg cccattgcag aaaagtattt cgcgtttgtg
    21181 ggtcagccag tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg
    21241 gaatagcatc cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg
    21301 caaattgcgg tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat
    21361 aatcaatcgt ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg
    21421 tcgcatcgcc atccaacaat tcaaggcgcg gtgcggcatc aaaatggtta gcttcccgct
    21481 cattctccag gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga
    21541 ccaccagcgg tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct
    21601 caacgcgaaa cactaactga tgcggtccgt tatccagttc aatgctgtcc gcaccgcgca
    21661 gcagagagct ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc
    21721 gcagcgtggt ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc
    21781 cggttttcat aggtagctcc tgtcaaaaga ccgcccgaat aataatgt
//
LOCUS       DAZFRG010000062        21756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFRG010000062 DAZFRG010000000
VERSION     DAZFRG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.062"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            103..816
                     /gene="yghS"
                     /locus_tag="ACHKUC_001828"
     CDS             103..816
                     /gene="yghS"
                     /locus_tag="ACHKUC_001828"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6618092.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNMTALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIQAALS"
     gene            848..1621
                     /locus_tag="ACHKUC_001829"
     CDS             848..1621
                     /locus_tag="ACHKUC_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618093.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTQSMKTKSSA
                     LWAAVIMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATG
                     KISMFLAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIG
                     YNGTKILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1667..3016
                     /locus_tag="ACHKUC_001830"
     CDS             1667..3016
                     /locus_tag="ACHKUC_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO6618094.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQIWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3016..3840
                     /locus_tag="ACHKUC_001831"
     CDS             3016..3840
                     /locus_tag="ACHKUC_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618095.1"
                     /translation="MMRKYFPLEASERLFVAVEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3852..4412
                     /locus_tag="ACHKUC_001832"
     CDS             3852..4412
                     /locus_tag="ACHKUC_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6618096.1"
                     /translation="MSSRLIIALIIMLLAQGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4443..5513
                     /gene="lptF"
                     /locus_tag="ACHKUC_001833"
     CDS             4443..5513
                     /gene="lptF"
                     /locus_tag="ACHKUC_001833"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6618097.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGNPGRLYKA
                     VLYLAILLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5510..6589
                     /gene="lptG"
                     /locus_tag="ACHKUC_001834"
     CDS             5510..6589
                     /gene="lptG"
                     /locus_tag="ACHKUC_001834"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6618098.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6624..7796)
                     /locus_tag="ACHKUC_001835"
     CDS             complement(6624..7796)
                     /locus_tag="ACHKUC_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6618099.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7796..8044)
                     /locus_tag="ACHKUC_001836"
     CDS             complement(7796..8044)
                     /locus_tag="ACHKUC_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6618100.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8076..8990)
                     /locus_tag="ACHKUC_001837"
     CDS             complement(8076..8990)
                     /locus_tag="ACHKUC_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6618101.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADAITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8987..10675)
                     /locus_tag="ACHKUC_001838"
     CDS             complement(8987..10675)
                     /locus_tag="ACHKUC_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO6618102.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGIPV
                     AERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
     gene            11079..12221
                     /gene="yghO"
                     /locus_tag="ACHKUC_001839"
     CDS             11079..12221
                     /gene="yghO"
                     /locus_tag="ACHKUC_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO6618103.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(12228..12992)
                     /gene="glcC"
                     /locus_tag="ACHKUC_001840"
     CDS             complement(12228..12992)
                     /gene="glcC"
                     /locus_tag="ACHKUC_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO6618104.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLEFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            13243..14742
                     /gene="glcD"
                     /locus_tag="ACHKUC_001841"
     CDS             13243..14742
                     /gene="glcD"
                     /locus_tag="ACHKUC_001841"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO6618105.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNQAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTLKLLPKPPMARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            14742..15794
                     /gene="glcE"
                     /locus_tag="ACHKUC_001842"
     CDS             14742..15794
                     /gene="glcE"
                     /locus_tag="ACHKUC_001842"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO6618106.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            15805..17028
                     /gene="glcF"
                     /locus_tag="ACHKUC_001843"
     CDS             15805..17028
                     /gene="glcF"
                     /locus_tag="ACHKUC_001843"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO6618107.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            17033..17437
                     /gene="glcG"
                     /locus_tag="ACHKUC_001844"
     CDS             17033..17437
                     /gene="glcG"
                     /locus_tag="ACHKUC_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO6618108.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            17459..19630
                     /gene="glcB"
                     /locus_tag="ACHKUC_001845"
     CDS             17459..19630
                     /gene="glcB"
                     /locus_tag="ACHKUC_001845"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO6618109.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHSILTKEQVQASLENMAKVVDQQNAGDPAYRPMSG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            19985..21667
                     /gene="glcA"
                     /locus_tag="ACHKUC_001846"
     CDS             19985..21667
                     /gene="glcA"
                     /locus_tag="ACHKUC_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO6618110.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGMGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         5167 a         5402 c         5896 g         5291 t
ORIGIN      
        1 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
       61 gaccacgtaa tattcaatac attatttgct gccgggtttt tcgtgaatca acgtaatatg
      121 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      181 aaatcgaccc tcacggcaag cctggtaaat gaactggcag caacaatgcc aacagaacac
      241 atttatctcg ggcaatcgtc cgggcgaatt ggcgaatgga tttcacagct ccctgttatt
      301 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaagccctcc
      361 acaccgcctg gcaatatgac tgcgctggtt atctatctgc tttcctgctg gcgggcgtat
      421 aagtttcgca aaatgttgtg taaaagccag caaggctatc tgctcatcac cgaccgctac
      481 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      541 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      601 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      661 aaacctgacc accaactggc ggcgttacag gaaaaaatcg ccgttacgcc gcaactgaca
      721 tttaatggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aattctgcaa
      781 gcgtcactac gcgcaattca agctgcgctt tcctgatcac caaaatggat aggggatgat
      841 ttaacgaatg gacgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccaa
      901 ctacattccg gggctgattg cggtggtcgg gtgtgatggc accggtaaat ccacactgac
      961 caccgacctc gtgaaatcgc tgcaacaaca ctggcaaacc gagcggcgct atctggggct
     1021 gctctcgggc gaagacggcg acaaaatcaa acgattgccg ttggttggcg tctggctcga
     1081 aaggcgactg gcggccaaat catcgaaaac ccaaagcatg aaaacccaaa gcatgaaaac
     1141 caaatcttca gcgctatggg cggcggtgat tatgtactgc ttctcgctgc gaagaatggc
     1201 gaatctacgc aaggttcagc gactggcgca aagtggcgtt ctggtggtca gcgatcgctt
     1261 cccgcaggct gaaatttcgg gcttttatta tgacggaccg gggatcggcg tcgaacgtgc
     1321 gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg cgtttatacc aaaaaatggc
     1381 gcaataccgt cctgaattaa tcattcgttt ggggattgat attgatactg ccatctcccg
     1441 caagcctgac catgactatg ccgagctgca ggacaaaatc ggcgtcatgt cgacgattgg
     1501 ctataacggc acaaaaattc ttgagattga ttccagagcg ccctacagcg aagtgctgga
     1561 acaggcacag aaggcggttt ctctggttgc catcgtttct gaccgccgaa gtttaactta
     1621 aggctggcgg gatacttttt actatcaacg ataaggtcag gctgaattgg cgggttttaa
     1681 catcaaacat tggtttgcag atggcgcgtt tcgcaccatt attcgcaata gcgcctggtt
     1741 aggctccagt aatgtcgtga gcgctttact gggtctgttg gcgctctcgt gtgccggtaa
     1801 agggatgacg cccgccatgt ttggcgtact ggtgattgta caatcgtacg ccaagtcgat
     1861 cagcgatttt attaagtttc agatatggca gctggtggtt cagtacggaa caccagcatt
     1921 aaccaacaat aatccgcagc aattccgcaa tgtcgtctca ttttccttct cgctggatat
     1981 cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta ctcccttttc tttcccattc
     2041 attaggtctg gatgaccaaa gcttttggct ggcagcgctg tattgcacgc tcattccttc
     2101 aatggcttcc tccacgccga ccggcattct gcgtgcggta gatcgcttcg atctaattgc
     2161 tgtacagcaa gcgacgaaac cttttctgcg cgcagcgggg agcgtcgtag cctggtattt
     2221 tgactttggt tttgcgggtt ttgttattgc ctggtacgtg tcgaatctgg ttggcggcac
     2281 catgtactgg tggtttgccg cgcgcgaatt acgccgcaga aatatccata acgccttcaa
     2341 attgaatctg tttgagtctg cccgacacat taaaggcgcg tggagttttg tctggtcaac
     2401 caacattgcc cactccatct ggtcggcgcg taactcgtgc agcacagtgc tggtggggat
     2461 cgtgttagga cccgctgccg ccgggttatt taaaatcgcc atgacattct tcgacgccgc
     2521 cggaacgcca gcgggtttac tgggcaaaag tttttaccca gaggtgatgc gtttagatcc
     2581 gcgcaccacc agaccgtggt tgttaggcgt gaagtcaggt ttactggcgg gcggaattgg
     2641 tattctggtg gcgctcgcgg tgttgatcgt cggcaagccg cttatttcgc tggtgtttgg
     2701 cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa ttgtgatttc
     2761 gatgctgggc ttcccacagg agtcattgct attgatggcg ggaaaacagc gcgcttttct
     2821 cgtggcgcaa accatcgcct caatcggcta catcgtattg ctgttcatgt tctgtcatct
     2881 gtttggcgtg ctgggagcag cgtttgctta tttcggtggg caatgtctgg atgtggtgct
     2941 ctcgttaatt ccgaccttaa aggcattttt tcagcgccat tccttgcttt ataacgcagc
     3001 cggagagaaa tcctgatgat gagaaaatat ttcccgcttg aggcgagcga gcgtttattt
     3061 gttgcagttg aagaagatga tgtcgttgat gcacaggtca gtttgccgcc gactatcgca
     3121 ctaagttgta ccaccgaaat catccatgat aattacgcgt tatgcctgca attctggctc
     3181 aatggcgtca atcgccagga gttactgcgt ttaatatgca aacaggccaa aggcgatgag
     3241 ctgactgctg atgagcgcaa acaatttaaa tacatgcgag cgcgctacaa gcacctgcgt
     3301 tttgcgcaac ggctgtattt aaagaagcat caggccggat ttttattcgg caaaacgacc
     3361 gtttttttgg ggcgttttca ggacggtttt cgtaacggta agaaaaatat tgtgtcgtat
     3421 tacggcaact tgttacgggt ttatttaagc tctccggtct ggtcgttggt gaactattca
     3481 ttacgccata gccagttaga aagcgtaagc ggttttattg cctatcggca aaagcaaatg
     3541 cataccctga aagagataat tgccaaaccg cgactcaccg gcagggaatt tcatgatgtg
     3601 cgaaaaatca tcagccagca ggtttcttat tacgacacct taagatcgct tgatcctgaa
     3661 aataaagagg cgttacagat ttcccgcttc ctcgccgcca ttaatggcct gatgggggat
     3721 aaacacgacg acatggtggc ggatgatatg gaaaaccgcc agtcgtatga tgcaccggtg
     3781 gcgctggata gcgatattcg ccagcgctta gagctgctga tttcgcgttt tccgctataa
     3841 ccggagcaat gatgtcatct cgtttgatta ttgcgctgat tatcatgctc ttagcccagg
     3901 gtgtgcaggc acacaatttt gtcactggca aaacggttac ccccgtttat atccaggaag
     3961 gcggcgagct gcttctgaat agcgacgacg aaattcacta ccaaaagtgg aacagcacac
     4021 agctggcggg aaaagtccgc attatccagt atatcgccgg gcgtaagtcg gcgaagaaaa
     4081 agaactcact gttaattaaa gcggttgaag cggcaaactt tccgcaggat cgttttcagc
     4141 ccaccaccat tgtgaacacc gatgatgcca tctttggtac tggctatttt gtcgttggca
     4201 aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt cgttattgac ggcaatggac
     4261 tgggacgcgt tgcctggcgg ttgccggagc agagttcaac cattctggtg ctgaataaag
     4321 acgggcaaat tcagtgggca aaggacggca gcctgacgcc ggaagaggta gatcatgtca
     4381 ttgcgctggc gcagaaactg atcaatgaat aaaaaattca aaggattgag ggtgttagtg
     4441 agatgaagct ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg ctgattatcg
     4501 tcggttttct gatctttatt ttcgccagct actccgcaca gcgttatctg accgaagcgg
     4561 caaatgggac cttagctctt gatgttgtgc tggatatcgt tttctacaaa gtgctgattg
     4621 cactggagat gttgttacct gttggtctgt atgtgtcagt tggcgtgacg ctagggcaga
     4681 tgtataccga ctcggaaatt accgctatct ctgcggcggg gggcaatccg ggacgcttgt
     4741 acaaagccgt tctttatctg gcgatactgc taagtatttt tgtcaccctt ctgtcgatgt
     4801 atggtcggcc gtgggcttat gcgcagattt atcaactgga gcaacagtcg cagtcggagc
     4861 tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa cgataacgga cgaatgatcc
     4921 tttcgcagac ggttgatcag gataataatc gcctgactga cgcgctgatt tatacttcta
     4981 ctgccaatcg aacccgcatt ttccgcgccc gttcggttga tgtggttgac ccatcacctg
     5041 agaaaccgac cgttatgttg cataacggga ccgcctatct tctcgatcat caggggcgtg
     5101 acgacaacga acagatctac cgtaatctgc aattacatct gaatccgctg gatcaaagcc
     5161 ctaacgtcaa acgcaaagca aaatcggtca cggagctggc gcgctcagcc tttcctgccg
     5221 atcatgccga actgcaatgg cgacaaagcc gtggcctgac agcattgttg atggcgctgc
     5281 tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg attttcaacg ttattgccac
     5341 tgacattgct gtttgttgcc attttttatg gcggcgacgt ctgccgtacg ctggtggcta
     5401 acggtgcgat tcccctcatt cctggtttgt ggttagtacc cggactcatg ctaatgggcc
     5461 tgctgatgct ggtcgcacgc gacttctctt tgctgcagaa attttcccga tgaatgtttt
     5521 cagtcgctat ttaatccgtc atctctttct cggttttgcc gccgccgcag ggctattgct
     5581 gccgcttttt accaccttca acctgattaa cgaactggat gatgtcagcc cgggcggtta
     5641 tcgctggact caggcggtgc tggtggtgct gatgacctta ccgcgtacac tggtcgaact
     5701 ttcgccgttt atcgcattat tgggagggat tgttggcctg gggcagttat cgaaaaacag
     5761 tgagcttacc gccattcgca gcactgggtt ttctatcttc cgtattgcac tggtggcgct
     5821 ggttgccggg atattgtgga ctgtttcgtt aggcgcgata gatgagtggg tggcgtcgcc
     5881 attgcagcaa caggcgctgc aaatcaaatc gactgccacc gcgttggggg aggacgatga
     5941 cattaccggc aatatgcttt gggccaggcg cggtaatgaa tttgtgacgg tgaaatcgct
     6001 gaacgagcag ggccagcctg tgggcgtgga gatatttcat tatcgcgacg atctctcgct
     6061 cgaatcctac atttttgcac gcagtgcctc cattgaagac gacaaaacgt ggatcctgca
     6121 tggtgtgaat cataaaaaat ggttgaatgg caaagaaacg ctggaaacat cagataatct
     6181 tgcctggcaa tcggccttca ccagtatgga tcttgaagag ttatcgatgc cggggaatac
     6241 tttttctgtc cgtcagctta atcattacat ccattatttg caggaaaccg gacaacccag
     6301 cagcgaatac cgccttgcac tgtgggaaaa actggggcaa ccgatcctga ccctggcgat
     6361 gattttgctg gctgtgccgt tcacctttag cgccccgcgc tcgccaggga tgggtagccg
     6421 tctcgctgta ggcgtcatcg ttggcttact cacctggatc agctatcaaa tcatggtcaa
     6481 tctgggattg ttatttgcgt tgagcgcacc tgttaccgcg ctcggtttac cggtagcgtt
     6541 tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga caacattaaa cccgccgtgg
     6601 cgcaacacca cagcgggctc acattactgc ttcagagtag caaatgcctg aatgatggca
     6661 tcgatttgct cacgactgtg ggcagcgttt acgctacagc gcagcagagt gatgcctgca
     6721 ggcgcggcag gtggcaggat aagattcaca taaacgccca gcgaaataag ctcgcgccag
     6781 atgcgtaaac cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa
     6841 cttaactcat aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagt
     6901 ttttgccgca gctctggttg tgtggcaatg gttcttaacg acgagcgcac ggtcgcaatg
     6961 caggacggag aaggagaggc ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc
     7021 atcgcttccg aaccgacagc aaaaccgcca atcgaggcca gacttttact gaaagtacca
     7081 acgataatat ccacatcatc ttcaacgcca acggcttcag ccagtccgcg ccccgttgcg
     7141 cctaacacgc caaacgaatg cgcttcatcg acaatcaaat aaccaccgag acgacgtttg
     7201 atatcaacaa tttccgctaa cggcgcaacg tcaccgagca tgctgtagat gccctcaacg
     7261 atgataatcg cctctttcgc ccgttcaccg aggcgaacca tgcgccgttc cagatcttta
     7321 gcatcgttat ggcggaaacg aataatttct gcgccaccga gagaacaggc gtcataaata
     7381 ctggcgtggc tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgcgctgatg
     7441 acgcctaaat tagcggtata accggttgaa aaaacgatag cggtagggcg attgaaaaac
     7501 tcggcaatct ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc catacgagaa
     7561 ccggtggtgc cggtgccttg ggccgctaac gctgcctgac cttcggcgat ggcatcatga
     7621 ttaaaggtta aaccgagata attattggtc ccggccagaa tcactttttt attgccgatc
     7681 cgaccttcgg tggcggaata cacttcatca atacaggtgc caaacggtgt taacccgttg
     7741 tcactaaatt gcagtcgctc gccagcaagg cgcgcatatt tatcgtatag ccccattatt
     7801 tattctccag ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca
     7861 gaatattaat aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca
     7921 ctttgatcga ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac
     7981 cgttttcgac caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta
     8041 ccatagaaac gttaacacac ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg
     8101 tgttccaggc taatcccggg aaaccaatta atatcttcag aaatacggtt attacttgcc
     8161 gaccagtcgg catgggttaa ttcacggatt ttcgaacgag tcagcatcgg ttctttaccc
     8221 gccagacggc tcaacgcggt actgatatcc gcaaggcagg tgagtagcgg cagaggaata
     8281 ccgaccatcc gcacggaacc acagcggaca tcggcaacaa gctgttggac acgttgccag
     8341 tcatagcctc cggcgacgcc atcgcataat tcataggttt gcgtctgtac agtttcggcg
     8401 cttaaccact gacccactgc ttgcgcaaaa tcggtgacgt gcaggaaaga gagctgtgtc
     8461 tctggcgtgc caagtcgtgg cagcagaccg cgcagcatcc agtcaaacag cggttttaac
     8521 tctttatcgc cgggaccata cacggctgtc gggcgaaaaa cgcccaacgt aattgcgtca
     8581 gccattgcag tcagctgttg ttcggcgacg tgtttggaat ttgcgtacca ggagagttcg
     8641 ggatggcgcg ccgccagcga agagataaac agaaatcgtt ggcaaaagcc gctctctttt
     8701 gccgcttgca tcaggcgcag gctgccgtca acgttgcagc gggtaaaaat ctcttctttg
     8761 tgcccgcgca cttgcccggc gcaatggacc accgcgctgg ctccggtaac cagttcgcta
     8821 agcgaatgtg tatcttccag cgaaccgcgc acccaagtga gattatcatt gacgtgagcg
     8881 cgggcagtac gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcaataata
     8941 tatttaccga taaacccggt agcgcccgtc accgcgacag tttgattcat gccagggact
     9001 cctgcacatg aagactggca gcataagcct tctgataacg ttttttcgct tccgcacggg
     9061 caggcttgcc ggaggatgtt cggggaatac tgtggggcgg caacagatcg atatccgcgg
     9121 taacgccaaa ttcgctttgg atccgtgctg ccagcgcgtg gataagctgc ccgcgacgtt
     9181 cttcatcgct gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag
     9241 caatcgcatc gccagaatga atttccggtt cctgttccgc aatatattca atatcctgcg
     9301 gccagatatt acggccacga ataataatca gatctttaat gcgtccggtg acatacagat
     9361 aaccgtccag cagataaccg aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt
     9421 cttgtgaaat ctggtcgcca aagtaaccgc tcatcaggct agggccggag atgcaaatat
     9481 ggcctaccac acgttccgcg acaggtatac ctgcttcatt gcggatttca atgccatgtt
     9541 ccggcaacgc tttaccgcag ttgacgaaag tcgatacggc gcgtgtttca gcacccggcg
     9601 cgacggcttt accctggtat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg
     9661 aagcttcatc agagaagctg acagccagcg cattttctgc cagtccgtag cacggcatga
     9721 aagttttatc gtcaaagttg acctgacgga aacattcagc gaattgatgg agttgttctg
     9781 cggagatggg ttctgcacca ataccagcga cgcgccagca ggaaagatcc agttcagcga
     9841 gatctttttc attcacgcgg cgctggcaca attcatagcc aaacggcggc gcaacggaaa
     9901 cggtgccgcg atttttactg atcaatttaa gccattgcag aggacgcatg gcaaaatcct
     9961 gagtgcgcaa ataatctact gaaagctgcg tggcgacggg ggtcaggaga aagccgacca
    10021 gtcccatatc atggtagaaa ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa
    10081 tcccatcgtg gcttatagca cgtagattag ccatcacttc gcggtgagta atgataacgc
    10141 cacggggaaa acgggtgctg ccggaggtgt actggaggta ggcgatatcg tttggaactg
    10201 gacgttgaag cgcaacatcg gcttccggta atgccttaaa ccaggcgtgg cttaaaacat
    10261 gtaattcggg attgttatgc gtcgcggcat tgaccagtgg caaccactca tcaccagtga
    10321 taatggctgc gggctggcaa cttgccagta acccctgcag tttggcgctc caggaatccc
    10381 gctgaccgac gcccattgga atcgccaacg ggacggcgac taagccggca tactggcagg
    10441 caaaaaaagc ctctacaaac ccgctactcg tttcggcaat cagtgccacg cgatcgcctt
    10501 ttttcaggtt cagcgataac aaccgcttcg caccagcttc ggcacgcgct tttaacgtct
    10561 gatattccag ttgatcttca agttggcaac gtctgtcata aaaattcatt ccagcgctac
    10621 tcagagcggc atagtccaaa gcatcaacca gcgttggaaa atcggcatag cgcataggta
    10681 gggaatgcgt aaagatttta ttagacatat acaccacgaa aaaattggat atcaaaaata
    10741 aaatgacaat aatgcggtgg attatatagg cataaaagat aaatgagata gtattttcat
    10801 cggtgcgata ttgaacattg atttaaataa taggttttgt tttgttgttt ttattgtgaa
    10861 tgatatttga tttatgaatg tattaagaat gaaccttgtc tgaccgcatt aaatcgctaa
    10921 aaggcattgt ggcgcttctt cgtaggctgt gatcttatcg taatcattaa taaataatat
    10981 ttattcttat tttgtattca tggtgttgat tattaagatg ccgttcgttg ctgttgcaac
    11041 ctgtttttca tcgttaatta tttgttatcc acagagatat ggaatgcgat ctgctgatga
    11101 ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct tttccgtcat
    11161 cactttatcc cgacgatcca aactggatac cccctttatt cattgaacgc aacgagcatt
    11221 tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg gcaaaaaaag
    11281 cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgc gagcgttacg
    11341 gcaaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag gtttttgccg
    11401 cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atcagcggtc
    11461 ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttc gatacaccac
    11521 cctgtgcgat gatgccgcac ggtaaaccgt ggtatgccgc gcatattgaa caattgggtt
    11581 atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc
    11641 cggcgctaaa aaaactgatg gatcaggtgc gtaagaaggt gaccattcgc tgcatcaatc
    11701 gccagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaattct ggctggcagc
    11761 acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc gaccaactta
    11821 aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca ccctgcgcgt
    11881 ttattgtcgg cttgccaaac atcaacgagg cgattgccga tctgaacgga tcgctctttc
    11941 ccttcggctg ggcaaaattg ctgtggcgtt taaaagtcag cggtgtgcga actgcgcgag
    12001 taccgctgat gggcgtgcgg gacgagtatc agttcagccg catcggcccg gtgattgcgt
    12061 tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat gcgctggaga
    12121 tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc attggggcgg
    12181 agccgtacaa gcgttaccga ttgtatgaaa agcagatttg atggcagtca cccctgcttc
    12241 atctccagcg gcaccgccga gcgaatcaaa tggtggcctt ccagctcgat atcgtggaga
    12301 ttctttttca cggtccgcac atgatcgcgt gctgcgcgct gggcgacgtg cggcagccgc
    12361 tgcaacaccg cgttgtagat ccgcgcatgc tggcgatcga tctgcttttt ttgctgtggt
    12421 cgatggtaga gattatttac gctggcaaac accgaattaa acatcaggtc ggtcaatgat
    12481 tgcagcgtaa actccagcac ctgattgtga gaagcctgac aaatggcgag atggaaagcg
    12541 tgatccaact gcgcatgttc gatcagcgaa atctctttgt tgttctcact ggcggcgagc
    12601 attttttcat aacagcgggt tatcacaaca aaatcagcct gggttcccag cgttgccgcc
    12661 agccttgccg attcgccctc cagtaatgcg cgaacgtcga gcagatcgta cagcgttcgc
    12721 ggctgcgtac tgaacagatg gatcagcggg ctggtgtcct gcacccgatt aagccgtgcg
    12781 acacgagaat cgcgaccctg cgccgtttca ataatcccgc gcccgcgcag cacggtcagc
    12841 ccttcacgca gtgcggagcg tgagaagccg agcttttcac acagtcgacg ttccgaggga
    12901 agcggctgac cgaccttcag tacgccgtcg ataattaacc gttcgatact ctctgcaacc
    12961 acttcgcaaa tagggcgacg ttcatctttc attcccggac ctcgtgcaca ggtaggacca
    13021 atttttctgt tcttaactcg caaaacacgc acatcacgta agtgattatg ttacatcaat
    13081 ttaacattaa gttaaccaag acaaggtcac agagctggaa aaaaaatggt ctgaccggta
    13141 gctaaagaga tagacgaaaa cgaaaagccc gcttaataac tgttcacaga agcagcgcga
    13201 aaaaatcagc tgccacacaa cacaacaaag cgaagcctac tcatgagcat cttgtacgaa
    13261 gagcgtcttg atggcgcttt acccgatgtc gaccgcacat cggtactgat ggcactgcgt
    13321 gagcatgtcc ctggacttga gatccttcat accgatgagg agatcattcc ttacgagtgt
    13381 gacgggttga gcgcgtatcg cacgcgtcca ttactggttg ttctgcctaa gcagatggaa
    13441 caggtgacgg cgattctggc tgtctgccac cgcctgcgtg taccggtggt gacccgtggt
    13501 gcaggcaccg ggctttctgg tggcgcgctg ccgctggaaa aaggtgtgtt gttggtgatg
    13561 gcgcgcttta aagagatcct cgacattaac cccgttggtc gccgtgcgcg cgtgcagccg
    13621 ggcgtgcgta accaggcgat ctcccaggcc gttgcgccgc ataatctcta ttacgcgccg
    13681 gatccgtcct cacaaatcgc ctgttcgatt ggcggcaatg tggcggaaaa tgccggtggc
    13741 gttcactgcc tgaaatatgg tctaaccgta cataacctgc tgaaaattga agtgcaaacg
    13801 ctggatggcg aggcgctgac gctgggatcg gacgcgctgg attcgcctgg ttttgacctg
    13861 ctggcgctgt ttaccggatc ggaaggtatg ctcggcgtga ccaccgaagt gacgttaaaa
    13921 ctgctgccga agccgcccat ggcgcgggtg ctgttagcca gctttgactc ggtagaaaaa
    13981 gccggacttg cggttggtga catcatcgcc aatggcatta tccccggcgg gctggagatg
    14041 atggataacc tgtcgatccg cgctgcggaa gattttatac atgccggtta tcccgtcgat
    14101 gccgaagcga ttttattgtg cgagctggac ggcgtggagt ctgacgtaca ggaagactgc
    14161 gagcgggtta acgacatctt gttgaaagcg ggcgcgactg acgtccgtct ggcacaggac
    14221 gaagcagagc gcgtacgttt ctgggccggc cgcaagaacg cattcccggc agtggggcgc
    14281 atttccccgg attactactg tatggatggc accatcccac gccgcgcgct gcctggcgta
    14341 ctggaaggca ttgcccgttt atcgcagcaa tatgatttac gcgttgccaa cgtctttcat
    14401 gccggagacg gcaacatgca cccgttaatc cttttcgatg ccaacgaacc cggtgaattt
    14461 gcccgcgcgg aagagctggg cgggaagatc ctcgaactct gcgttgaagt tggcggcagc
    14521 atcagtgggg aacatggcat tgggcgcgaa aaaatcaatc aaatgtgcgc ccagttcaac
    14581 agcgatgaaa tcacgacctt ccatgcggtc aaggcggcgt ttgaccccga tggtttgctg
    14641 aaccctggga aaaacattcc cacgctacac cgctgtgctg aatttggtgc catgcatgtg
    14701 catcacggtc atttaccttt ccctgaactg gagcgtttct gatgctacgc gagtgtgatt
    14761 acagccaggc gctgctggag caggtgaatc aggcgattag cgataaaacg ccgctggtga
    14821 ttcagggcag caatagcaaa gcctttttag gtcgtcctgt caccgggcaa acgctggatg
    14881 tgcgttgtca tcgcggcatt gttaattacg acccgaccga gctggtgata accgctcgtg
    14941 ccggaacgcc gctggtggcg attgaagcgg cgctggagag cgccgggcaa atgctcccct
    15001 gcgagccgcc acattatggt gaagaagcta cctggggcgg gatgatcgcc tgcgggctgg
    15061 cggggccgcg tcgcccgtgg agtggttcgg tccgcgattt tgtcctcggc acgcgcatca
    15121 ttaccggcac tggaaaacat ctgcgttttg gtggtgaagt gatgaaaaac gttgccggat
    15181 acgatctctc acggttaatg gccggaagct acggttgtct gggtgtgctc actgaaatct
    15241 cgatgaaagt gttaccgcga ccacgcgcca ccctgagtct gcgtcgggaa atcagcctgc
    15301 aagaagccat gaatgaaatc gcccagtggc aactccagcc attacccatt agtggcttat
    15361 gttacttcga caatgcgttg tggatccgcc ttgagggcgg cgaaggatcg gtaaaagcag
    15421 cgcgtgaact gctggggggc gaagaggttg cgggtcagtt ctggcagcaa ttgcgtgaac
    15481 aacaactgcc gttcttctcg ttaccaggta ccttatggcg catttcctta cccagcgatg
    15541 cgccgatgat ggatttaccc ggcgagcaac tgatcgactg gggcggggcg ttacgctggc
    15601 tgaaatcgac agccgatgac aatcaaatcc atcgcattgc ccgcaacgct ggcggtcatg
    15661 cgacccgctt tagtgccgga gatggtggtt ttgccccgct accggctcct ttattccgct
    15721 atcaccagca gcttaaacag cagctcgacc cttgcggcgt gtttaatccc ggtcgcatgt
    15781 acgcggagct ttaaggagca ggctatgcaa acccaattaa ccgaagagat gcggcagaac
    15841 gcgcgggcgc tggaagccga cagcatcctg cgcgcctgtg ttcactgcgg attttgtacc
    15901 gcaacctgcc caacctatca gctcctgggc gatgaactgg acgggccgcg cgggcgcatc
    15961 tatctgatta aacaggtgct ggaaggcaac gacgtcacgc ttaaaacaca ggagcatctc
    16021 gatcgctgtc tcacttgccg caattgtgaa accacctgtc cttctggtgt gcgctatcac
    16081 aatttgctgg atatcgggcg tgatattgtc gagcagaaag tgaaacgccc actgccggag
    16141 cgaatgctgc gcgaaggatt gcgccaggta gtgccgcgtc cggcggtctt ccgtgcgctg
    16201 acgcaggtag ggctggtgct gcgaccgttt ttaccggaac aggtcagagc aaaactgcct
    16261 gctgaaacgg tgaaagctaa accgcgtcca ccgctgcgcc ataagcgtcg ggttttaatg
    16321 ctggaaggct gcgcccagcc tacgctttcg cccaacacca acgcggcaac tgcgcgagtg
    16381 ctggatcgtc tggggatcag cgtcatgcca gctaacgaag caggctgttg tggcgcggtg
    16441 gactatcatc ttaatgcgca agagaaaggg ttagcgcggg cgcgcaataa tattgatgcc
    16501 tggtggcccg cgattgaagc aggtgccgag gctattttgc agactgccag cggctgcggc
    16561 gcgtttgtca aagagtatgg gcagatgctg aaaaacgatg cgttgtatgc cgataaagcg
    16621 cgtcaggtca gtgagctggc ggtcgattta gtcgaacttc tgcgcgagga accgctggaa
    16681 aaactggcaa ttcgcggcga taaaaaactg gccttccact gcccgtgtac cctacaacat
    16741 gcgcaaaagc tgaacggcga agtggaaaaa gtgttgcttc gtcttggatt taccttaacg
    16801 gacgttcccg acagccatct gtgctgcggt tcagcgggaa catatgcgtt aacgcatccc
    16861 gatctggcgc gccagctgcg ggataacaaa atgaatgcgc tggaaagcgg caaaccggaa
    16921 atgatcgtca ccgccaacat tggttgccag acgcatctgg cgagcgccgg tcgtacctct
    16981 gtgcgtcact ggattgaaat tgtagaacaa gcccttgaaa aggaataaca aaatgaaaac
    17041 taaagtcatt cttagccagc aaatggcgag tgcaattatt gccgcaggtc aggaagaggc
    17101 gcagaaaaac aactggtctg tttccattgc tgttgccgat gacggcggtc atctgctggc
    17161 gttaagtcgc atggacgatt gcgcgccgat tgcggcttac atctcccagg agaaagcgcg
    17221 taccgccgcg ctggggcgtc gtgaaactaa ggggtatgaa gagatggtga acaacggacg
    17281 taccgcgttc gtgactgcgc cgttattaac gtcgctagaa ggtggcgtac cggttgttgt
    17341 ggatgggcaa attattggtg ccgtgggcgt ttctggttta accggagcac aggatgcgca
    17401 ggtcgcgaaa gcggcagcag cggtgttggc gaaataagcg aaaacgagga gagaaacaat
    17461 gagtcaaacc ataacccagg gccgtttacg cattaacgcc aattttaaac gttttgtgga
    17521 tgaagaagtt ttaccgggaa cagggttgga cgctgcggcg ttctggcgca attttgatga
    17581 gatcgttcac gatctggcac cagaaaatcg tcagttgctg gcagaacgcg atcgcattca
    17641 ggcggcgctt gatgagtggc atcgcagcaa tccggggccg gtaaaagata aagcggccta
    17701 taaatctttc ctgcgtgaac tgggctacct ggtgccgcaa ccggagcgcg taacggtgga
    17761 aaccacgggc attgacagcg aaatcaccag tcaggcgggg ccacagctgg tggttccggc
    17821 aatgaacgcc cgctacgcgc tgaacgcggc gaacgctcgc tggggctcac tgtacgatgc
    17881 gttatacggc agcgacatca tcccgcagga aggggcgatg gtcagcggct acgatccgca
    17941 gcgtggtgag caggttatcg cctgggttcg gcgtttcctc gatgaatcac taccgctgga
    18001 aaacggcagc tatcaggatg tggtggcgtt taaggtggtc gataaacaat tacgcatcca
    18061 gttgaaaaat ggtaaagaaa ccacgttacg taccccggcg cagtttgtcg gttaccgtgg
    18121 cgatgccgct gcgccgacct gcattttgct gaaaaataac ggtctgcata ttgaactgca
    18181 aattgatgcc aacgggcgga ttggcaaaga cgatccggcg catatcaacg atgttatcgt
    18241 cgaagcggcc atcagtacca ttctcgactg cgaagattcg gtcgcggcgg ttgatgcgga
    18301 agataaaatc ctgctgtacc gcaacctgct gggcctgatg caggggactc tgcaagagaa
    18361 aatggaaaaa aacggtcggc aaatcgtgcg taaactgaat gacgatcgtc attacaccgc
    18421 cgccgatggc tctgaaattt ctctgcacgg acgctcgctg ctgtttatcc gcaacgtggg
    18481 tcatttgatg accattcctg tgatttggga cagcgaaggc aatgaaatcc cggaaggcat
    18541 tcttgatggc gtcatgactg gcgcgattgc cctctatgat ttaaaagtgc agaaaaactc
    18601 gcgcactggc agcgtctata ttgtgaaacc gaaaatgcac ggtccgcagg aagtggcgtt
    18661 cgccaacaaa ctgtttaccc gcattgagac aatgctcggt atggcaccga ataccctgaa
    18721 aatgggcatt atggatgaag aacgtcggac ctcgctgaac ttgcgtagct gtatcgctca
    18781 ggcgcgtaac cgcgtggcgt tcatcaatac cggtttcctc gaccgtaccg gcgatgaaat
    18841 gcattcggtg atggaagctg gcccgatgct gcgtaaaaac cagatgaaat cgacgccgtg
    18901 gatcaaagcc tacgaacgta ataacgtgct ttccggtctg ttctgtgggc tgcgcggtaa
    18961 agcgcaaatc ggtaaaggga tgtgggcaat gccggacctg atggcagaca tgtacagcca
    19021 gaagggcgac caactgcgtg ccggggcaaa cacagcctgg gttccgtcac caaccgctgc
    19081 tacgctccat gcgctgcact accaccaaac caacgtacag agcgtgcagg ccaacattgc
    19141 ccagagcgag ttcaatgctg aatttgaacc gctgctggac gatctgctga ctattccggt
    19201 ggctgaaaac gctaactggt cggttgaaga gatccaacaa gagctggata acaacgtgca
    19261 ggggattctg gggtacgtgg tgcgttgggt ggagcagggg attggttgtt caaaagtgcc
    19321 ggatattcac aacgtggcgc tgatggaaga ccgcgcaacg ctgcgtatct ccagccagca
    19381 tatcgccaac tggttacgtc acagtattct gaccaaagaa caggtgcagg cgtcgctgga
    19441 gaatatggcg aaagtggttg atcagcaaaa cgctggcgat ccggcttatc gtccgatgtc
    19501 ggggaatttc gctaactcgt gtgcttttaa agctgccagc gatttaatct tcctcggcgt
    19561 gaaacagcca aacggctata ccgaaccgtt attacacgcc tggcgtttac gcgaaaaaga
    19621 aagtcattaa tagcgtcaaa aaagccccgg caagttttat tgtcggggct atgatagtaa
    19681 ctgcggtagt acatttatcc cggaaataac cggagcgatt atcagggttt aacaatatta
    19741 aatacccgta gcgcattact agcgggccta aaacaacaat atggacatct taacgatatt
    19801 ctgcgcctgc gtgtgcggaa cagaaaccct tacccgatat acttgttatg ccagaggtag
    19861 caattgcgtt agcagcgtac tgttgctgta aagtataacg cctatatttt acagttcagt
    19921 cctcttttgc ttattacaac cttaagtata tcaagcatat aaagataata agagactgaa
    19981 aaatatggtt acctggaccc aaatgtatat gccgatggga ggactggggc tatccgctct
    20041 ggtcgccctg atcccgataa tattcttctt cgttgcactc gcggtattac gtctgaaagg
    20101 acatgtcgcc ggagcaataa cccttatatt atctattctg attgcgatat tcgcctttaa
    20161 aatgccgatt gatatggcat ttgctgctgc gggctatggc tttatttatg gattatggcc
    20221 tattgcgtgg attattgttg cggcggtgtt cctctataaa ttaaccgttg ccagcggtca
    20281 gttcgatatt atccgcagct cggttatctc catcactgac gatcagcgtt tgcaggtgtt
    20341 actgattggt ttctcctttg gcgcgctgct ggaaggggcg gctggctttg gtgcgccggt
    20401 ggcgattacc ggtgcgctgc tggtgggcct gggcttcaaa ccgttatacg cggcggggct
    20461 gtgtctgatt gccaacactg cgccggtggc gtttggtgcg ttgggcgtgc cgattctggt
    20521 cgccggtcag gtaacgggaa tcgatccgtt ccacattggc gcaatggcgg gacgtcagtt
    20581 accgttcctg tcggttcttg tgccgttctg gctggtagca atgatggacg gctggaaagg
    20641 ggtgaaagag acgtggccag cggcgctggt tgctggggga agcttcgctg tcacgcagtt
    20701 ctttacctct aactatattg gtccggaact gccggatatt acttcggcgc tggtgagtat
    20761 cgtctcactc gctttattcc ttaaagtctg gcggccgaaa aataccgaaa cggcaatcag
    20821 catgggacaa tccgcaggtg cgatggtggt gaataaacca tcttctggcg gtcccgtgcc
    20881 ttcagaatat agtctggggc aaatcattcg tgcgtggtca ccgtttttaa tcttaacggt
    20941 gctggtcacc atctggacca tgaagccgtt taaagcgtta tttgctccgg gcggcgcgtt
    21001 ttattcactg gtgattaatt tccagatccc tcatttgcat caacaagtgt tgaaagcggc
    21061 acccattgtc gcccaaccaa cgccaatgga tgcggtgttt aaattcgacc ctctctcggc
    21121 tggcggcacc gctattttta ttgcggcgat tatctctatc ttcatcctcg gcatgggcat
    21181 caagaaaggg attggcgtct ttgccgaaac gctaattagt ctgaagtggc cgatattgtc
    21241 gattggcatg gtgctggcgt tcgccttcgt caccaactat tccggcatgt ccaccacgct
    21301 ggcgctggta ctggcaggta caggcgtgat gttcccgttt ttctcaccgt ttctcggctg
    21361 gctgggcgta ttccttaccg gctcggacac ctcctctaac gccctgtttg gttcactgca
    21421 atcgaccacg gcgcagcaaa tcaacgtctc tgacactctg ctggtggcag caaataccag
    21481 cggcggcgta actggcaaga tgatttcccc gcaatctatc gccgtggcct gcgccgcgac
    21541 gggcatggtg ggccgagaat ctgaactgtt ccgctacacc gtgaagcaca gtctgatctt
    21601 tgccagtgtg atcggcatta tcacgctgtt gcaggcgtat gtgtttaccg ggatgttagt
    21661 ttcgtaagtt aataaagccg gatgatcgtt aatatcatcc ggcttcgttt acattactct
    21721 gtcttattaa tggaaaatta tttcccgctt tattat
//
LOCUS       DAZFRG010000063        21579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFRG010000063 DAZFRG010000000
VERSION     DAZFRG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.063"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..436)
                     /gene="emrE"
                     /locus_tag="ACHKUC_001847"
     CDS             complement(104..436)
                     /gene="emrE"
                     /locus_tag="ACHKUC_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO6618111.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(781..1095)
                     /gene="fliE"
                     /locus_tag="ACHKUC_001848"
     CDS             complement(781..1095)
                     /gene="fliE"
                     /locus_tag="ACHKUC_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6618112.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1310..2968
                     /gene="fliF"
                     /locus_tag="ACHKUC_001849"
     CDS             1310..2968
                     /gene="fliF"
                     /locus_tag="ACHKUC_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6618113.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPTNQNN
                     RQQQSSTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2961..3956
                     /gene="fliG"
                     /locus_tag="ACHKUC_001850"
     CDS             2961..3956
                     /gene="fliG"
                     /locus_tag="ACHKUC_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6618114.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3949..4635
                     /gene="fliH"
                     /locus_tag="ACHKUC_001851"
     CDS             3949..4635
                     /gene="fliH"
                     /locus_tag="ACHKUC_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6618115.1"
                     /translation="MSDNLPWKTWTLDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4635..6008
                     /gene="fliI"
                     /locus_tag="ACHKUC_001852"
     CDS             4635..6008
                     /gene="fliI"
                     /locus_tag="ACHKUC_001852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6618116.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6027..6470
                     /gene="fliJ"
                     /locus_tag="ACHKUC_001853"
     CDS             6027..6470
                     /gene="fliJ"
                     /locus_tag="ACHKUC_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6618117.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6467..7594
                     /gene="fliK"
                     /locus_tag="ACHKUC_001854"
     CDS             6467..7594
                     /gene="fliK"
                     /locus_tag="ACHKUC_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6618118.1"
                     /translation="MIRLAPLITANVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTIAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLLVPVSLQGRVTGNSGVDIFA"
     gene            7699..8163
                     /gene="fliL"
                     /locus_tag="ACHKUC_001855"
     CDS             7699..8163
                     /gene="fliL"
                     /locus_tag="ACHKUC_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO6618119.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8168..9172
                     /gene="fliM"
                     /locus_tag="ACHKUC_001856"
     CDS             8168..9172
                     /gene="fliM"
                     /locus_tag="ACHKUC_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO6618120.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9169..9582
                     /gene="fliN"
                     /locus_tag="ACHKUC_001857"
     CDS             9169..9582
                     /gene="fliN"
                     /locus_tag="ACHKUC_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO6618121.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGALQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9585..9950
                     /gene="fliO"
                     /locus_tag="ACHKUC_001858"
     CDS             9585..9950
                     /gene="fliO"
                     /locus_tag="ACHKUC_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO6618122.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            9950..10687
                     /gene="fliP"
                     /locus_tag="ACHKUC_001859"
     CDS             9950..10687
                     /gene="fliP"
                     /locus_tag="ACHKUC_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6618123.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10697..10966
                     /gene="fliQ"
                     /locus_tag="ACHKUC_001860"
     CDS             10697..10966
                     /gene="fliQ"
                     /locus_tag="ACHKUC_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO6618124.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNQLLDYVRTLFTNLPYIIG"
     gene            10975..11760
                     /gene="fliR"
                     /locus_tag="ACHKUC_001861"
     CDS             10975..11760
                     /gene="fliR"
                     /locus_tag="ACHKUC_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6618125.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12050..12673
                     /gene="rcsA"
                     /locus_tag="ACHKUC_001862"
     CDS             12050..12673
                     /gene="rcsA"
                     /locus_tag="ACHKUC_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO6618126.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12717..12905)
                     /gene="dsrB"
                     /locus_tag="ACHKUC_001863"
     CDS             complement(12717..12905)
                     /gene="dsrB"
                     /locus_tag="ACHKUC_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO6618127.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13068..13295
                     /gene="yodD"
                     /locus_tag="ACHKUC_001864"
     CDS             13068..13295
                     /gene="yodD"
                     /locus_tag="ACHKUC_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO6618128.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13591..14406
                     /gene="yedP"
                     /locus_tag="ACHKUC_001865"
     CDS             13591..14406
                     /gene="yedP"
                     /locus_tag="ACHKUC_001865"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO6618129.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(14403..16097)
                     /gene="dgcQ"
                     /locus_tag="ACHKUC_001866"
     CDS             complement(14403..16097)
                     /gene="dgcQ"
                     /locus_tag="ACHKUC_001866"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO6618130.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFISLRNAV
                     TEFEQHRDEHAWQIELNRRRTMPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRIDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCHTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDIAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(16268..16450)
                     /gene="yodC"
                     /locus_tag="ACHKUC_001867"
     CDS             complement(16268..16450)
                     /gene="yodC"
                     /locus_tag="ACHKUC_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO6618131.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(16529..17446)
                     /gene="yedI"
                     /locus_tag="ACHKUC_001868"
     CDS             complement(16529..17446)
                     /gene="yedI"
                     /locus_tag="ACHKUC_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO6618132.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     ILNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17619..18539
                     /gene="yedA"
                     /locus_tag="ACHKUC_001869"
     CDS             17619..18539
                     /gene="yedA"
                     /locus_tag="ACHKUC_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO6618133.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18528..18998)
                     /gene="vsr"
                     /locus_tag="ACHKUC_001870"
     CDS             complement(18528..18998)
                     /gene="vsr"
                     /locus_tag="ACHKUC_001870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO6618134.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18979..20397)
                     /gene="dcm"
                     /locus_tag="ACHKUC_001871"
     CDS             complement(18979..20397)
                     /gene="dcm"
                     /locus_tag="ACHKUC_001871"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO6618135.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20464..21159)
                     /gene="yedJ"
                     /locus_tag="ACHKUC_001872"
     CDS             complement(20464..21159)
                     /gene="yedJ"
                     /locus_tag="ACHKUC_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO6618136.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(21199..21480)
                     /gene="drpB"
                     /locus_tag="ACHKUC_001873"
     CDS             complement(21199..21480)
                     /gene="drpB"
                     /locus_tag="ACHKUC_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO6618137.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         5244 a         5714 c         5558 g         5063 t
ORIGIN      
        1 ggcgcaatga tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa
       61 tatgcacaaa tcagattcct gttatctcaa ggataattat tttttaatgc ggtgcacttc
      121 gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg
      181 gcaggtccag tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga
      241 ctcctgacca gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata
      301 accagaatga tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaatc
      361 cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat
      421 aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt
      481 ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccagggggct ctggcgtggc
      541 aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca
      601 ctcttcctgg gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc
      661 agaaaacaac caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc
      721 catgccccac gctgtgggat cggatataat taaacaagca tagtcaacag gttacacaac
      781 ctacacctgc atggacatca cttcctgata cgccgccacc agcttattac gcacctgaat
      841 ccccatttgc atagaaactg aggctttttg catatcggtc atcacatcgt ttagcgccac
      901 gccgggttca ccgagggtga atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct
      961 tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt gcggcagtga
     1021 ttcctgcgca cgcgcactca tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc
     1081 ctgtatcgct gacattttcg tctcccggat aatttctggt agcaaagcct accagtaagt
     1141 caataagaca aaggcgctaa atagcaacaa aaaaacgggt ttattggcgg atagaaaaaa
     1201 acgaaagcac aaataatggg agcgtcaatt tttcgagttt gctgacccgg gagtgagtct
     1261 tgttccactt tgccaataac gccgtccata atcagccacg aggtgcgcga tgaatgcgac
     1321 tgcagcccag acaaaatctc ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc
     1381 attgattgtt accggttccg cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa
     1441 agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg gcgcaattgt
     1501 cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg gcgctattga
     1561 ggttccggca gataaagttc acgaactgcg cctgcgcctg gcgcaacaag gtttgcccaa
     1621 aggcggcgcg gtcggtttcg aactgctcga tcaggaaaag tttggtatca gccagttcag
     1681 cgaacaggtg aattatcagc gggcgctgga aggcgagctt tctcgtacca tcgaaactat
     1741 cggcccggta aaaggggcgc gcgtacatct ggcaatgccg aaaccgtctt tattcgtccg
     1801 tgaacaaaaa tccccttctg catcggtgac ggtaaatctg ttacccggcc gcgcactcga
     1861 tgaagggcaa attagcgcta ttgtacatct ggtttccagc gcggttgctg gtctgccgcc
     1921 gggaaacgtc acgctggtgg atcagggcgg gcatctgtta acccagtcca ataccagcgg
     1981 gcgcgatctt aatgacgctc agttgaaata tgccagcgat gtcgaaggcc gtattcagcg
     2041 gcgtattgaa gcgatcctgt cgcctattgt tggtaacggt aatattcacg cccaggtcac
     2101 ggcgcaactg gacttcgcca gtaaagaaca aacggaagaa cagtatcgcc ctaacggtga
     2161 tgaatctcat gcggcgcttc gttcacgcca gcttaatgag agcgagcaaa gcggttccgg
     2221 ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg gcacctgcga ataacgcgcc
     2281 aatcagcacg cctccgacaa atcaaaataa ccgccagcag cagtcgagca ccaccagcaa
     2341 tagtgggccg cgtagcacac agcggaatga aaccagtaac tacgaagtcg atcgcaccat
     2401 tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg tcagtcgcgg tagtggtgaa
     2461 ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc aacgaacaga tgaagcaaat
     2521 tgaagctctg acccgcgagg cgatgggctt ttctgaaaaa cgcggtgatt cgctcaatgt
     2581 tgttaactcg ccgttcaata gcagtgacga aagcggcgga gcactgccgt tctggcaaca
     2641 gcaagtgttt atcgatcagt tgctggctgc cggtcgctgg ttgctggtac tgctggtggc
     2701 gtggttgctg tggcggaaag cggtacgccc gcagctaaca cgtcgcgccg aggcggtgaa
     2761 gactgtgcag caacaggcgc aggcccgcga ggaagtggaa gatgcggtgg aagtccgtct
     2821 gagcaaagac gaacaactcc aacaacggcg cgctaaccaa cgactggggg cagaagtcat
     2881 gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg
     2941 ccagtggata aataacgatc atgagtaacc tgacaggcac cgataaaagc gtcatcctgc
     3001 tgatgaccat tggcgaagac cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag
     3061 tgcaaaccct gagcgctgca atggcgaacg tcacgcagat ctccaacaag cagctaaccg
     3121 atgtgctggc ggagtttgag caagaagctg aacagtttgc cgcactgaat atcaacgcca
     3181 acgattatct gcgttcggta ttggtcaaag ctctgggtga agaacgtgcc gccagcctgc
     3241 tggaagatat tctcgaaacc cgcgataccg ccagcggtat tgaaacgctc aactttatgg
     3301 agccgcagag cgccgccgat ctgattcgcg atgagcatcc gcaaattatc gccaccattc
     3361 tggtgcatct gaagcgcgcc caggccgccg atattctggc gttgttcgat gaacgtctgc
     3421 gccacgacgt gatgttgcgt atcgccacct ttggcggcgt gcagccagcc gcgctggccg
     3481 aactgaccga agtactgaat ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg
     3541 gcggcgtgag aacggcagcc gaaattatca acctgatgaa aacgcagcag gaagaagccg
     3601 ttattaccgc cgtgcgtgaa ttcgacggcg agctggcgca gaaaatcatc gacgagatgt
     3661 tcctgttcga gaatctggtg gatgtcgatg atcgcagcat tcagcgtctg ttgcaggaag
     3721 tggattccga atcgctgttg atcgcgctga aaggggccga gcagccactg cgcgagaaat
     3781 tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg cgacgatctc gccaaccgtg
     3841 gtccggtgcg tctgtcgcag gtcgaaaacg aacagaaagc gattctgctg attgtgcgcc
     3901 gtcttgccga aactggcgag atggtgattg gcagcggcga ggatacctat gtctgataat
     3961 ctgccgtgga aaacctggac gctggacgat ctcgcgccac caccggcaga gtttgtgcca
     4021 atggtcgagt cggaagaaac cattattgaa gaggccgaac ccagccttga gcagcaactg
     4081 gcgcaactgc aaatgcaggc ccacgagcag ggttatcagg cggggattgc cgaaggtcgc
     4141 cagcaaggtc acgaacaggg ctatcaggaa ggactggccc aggggctgga gcaaggtctg
     4201 gcagaggcga agtctcaaca agcgccaatt catgcccgga tgcagcaact ggtcagcgaa
     4261 tttcaaacta cccttgatgc gcttgatagt gtgatagcgt cgcgcctgat gcagatggcg
     4321 ctggaggcag cacgtcaggt cataggtcag acgccaacgg tggataactc ggcactgatc
     4381 aaacagatcc aacagttgtt gcagcaagaa ccgttattca gcggtaaacc acagctgcgc
     4441 gtgcacccgg atgatctgca acgtgtggat gatatgctcg gcgcaacctt aagtttgcat
     4501 ggctggcgct tgcggggcga tcccaccctc catcctggcg gctgtaaagt ctccgccgat
     4561 gaaggcgatc tcgacgccag tgtcgccacc cgctggcaag aactctgccg tctggcagca
     4621 ccaggagtgg tgtaatgacc acgcgcctga ctcgctggct aaccacgctg gataactttg
     4681 aagccaaaat ggcgcagttg cctgcggtac gtcgctatgg gcgattaacc cgcgctaccg
     4741 ggctggtgct ggaagccacc ggattacaat tgccgctcgg cgcaacctgt gtcattgagc
     4801 gccagaacgg cagcgaaacg cacgaagtag aaagcgaagt cgttggcttt aacggtcaac
     4861 ggctgttttt aatgccgctg gaggaagtgg aaggtgtcct gcccggcgcg cgtgtatatg
     4921 ccaaaaacat ttcggcagaa gggctgcaaa gcggcaagca gttgccgctc ggtccggcgt
     4981 tattaggtcg tgttctggac ggtagcggta aaccgctcga tggcctgccc ttccccgata
     5041 cgacggaaac cggtgcgctg attaccccgc catttaaccc gttgcaacgt acaccgattg
     5101 aacatgtgct ggacaccggc gtgcgcccaa tcaatgccct gcttaccgtc ggacgcgggc
     5161 agcgtatggg gctgtttgcc gggtccggcg ttggtaaaag tgtgctgctg gggatgatgg
     5221 cccgttacac ccgcgccgat gtcattgtcg tgggtttgat tggtgaacga gggcgcgaag
     5281 taaaagattt tattgagaat atcctcggtg ccgaagggcg tgcacgctca gtggtgattg
     5341 ccgctccggc ggatgtttct ccgctcctgc gaatgcaggg tgccgcctac gccacgcgca
     5401 ttgccgaaga ttttcgcgat cgtggtcagc atgtgttgct gattatggac tccctcaccc
     5461 gctacgcgat ggcccagcgt gaaattgcgc tggcgattgg cgaaccacct gccactaaag
     5521 gttatccacc gtcggtgttt gccaaattac cggcactggt cgagcgtgcc ggtaatggca
     5581 ttagcggcgg cggctcgatt accgcgtttt ataccgtgct caccgaaggc gatgaccagc
     5641 aggaccccat tgctgactcc gcgcgggcaa tcctcgacgg tcacattgtg ctgtctcgcc
     5701 gactggcgga agccgggcac tatccggcta tcgatattga agcgtcgatc agtcgcgcaa
     5761 tgacggcgtt gatcagtgag caacattacg cgcgagtgcg caccttcaaa cagctgttgt
     5821 cgagttttca gcgtaaccgc gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc
     5881 cgatgctcga taaagccatc gccctgtggc cgcagctgga gggctatttg caacaaggca
     5941 tttttgaacg cgcggactgg gaagcgtctc tccaggggct ggagcgtatt ttcccgacag
     6001 tgtcataacc caggagataa cggcagatgg cggaacatgg tgcgctggcg accctgaaag
     6061 atctggcaga aaaagaggta gaggatgccg ctcgcctgct gggtgaaatg cgtcgcggat
     6121 gtcagcaggc ggaagaacag ctcaaaatgc tgatcgatta tcagaatgaa tatcgcaata
     6181 acctcaatag cgatatgagt gccgggatga ccagcaaccg ctggatcaac tatcagcagt
     6241 ttatccagac gctggaaaaa gccattactc agcatcgcca gcaacttaat cagtggacac
     6301 agaaagttga cattgccctg aacagctggc gagaaaagaa acaacgtttg caggcctggc
     6361 agacactgca ggaacggcaa tccacggcgg cactgcttgc agaaaaccgc ctcgatcaga
     6421 aaaagatgga tgagttcgcc cagcgcgccg ccatgaggaa acctgaatga ttcgcttagc
     6481 gcccttgatt accgccaacg ttgacaccac cacattgcct ggcggcaaag ccagcgatgc
     6541 tgcacaagat tttctcgcgt tgttgagcga agcattagca ggcgagacaa ctaccgacaa
     6601 agcggccccc cagttgctgg tagcaacaga taagcccacg acaaaaggcg agccgctgat
     6661 cagcgatatt gtttccgacg cgcaacaagc ggatttactg atccccgtgg atgaaacact
     6721 gcctgtcatc aacgacgaac aatccacatc aacaccgtta accaccgctc agacgatgac
     6781 gttggctgcg gtggctgaca aaaatacgac aaaagacgaa aaagcggatg atcttaatga
     6841 agacgtcacc gcaagcctga gcgccctttt tgcgatgttg ccgggttttg acaatacgcc
     6901 caaagtgact gatgcaccgt caaccgtgtt accggcagag aaaccaacgc tcttcacaaa
     6961 actgacttct gcgcaactca ctacagcaca gcctgatgac gcccccggca ccccagctca
     7021 gccattaaca ccgctggtag cagaagccca gagtaaagcg gaagtcatca gcacgccttc
     7081 gccggtgacc gctgacgcca gcccgctaat cactccacac cagacacagc cactgcccac
     7141 catcgccgcg cctgttttga gtgcaccgct gggttctcac gaatggcaac aatcattaag
     7201 ccagcatatt tcgctgttca cccgccaggg gcaacaaagt gcagagttgc gtctgcaccc
     7261 acaggattta ggtgaagtgc aaatttccct caaagtggat gataaccagg cgcagatcca
     7321 gatgatttca ccgcatcagc acgtacgcgc cgccctggaa gcagcgctgc cggtactgcg
     7381 tacgcagctg gccgaaagtg gcattcagtt agggcaaagc aacattagtg gcgaaagctt
     7441 tagtggtcag cagcaggccg cttcccagca acagcaaagc caacgcacag caaaccatga
     7501 acctctggcg ggggaagatg acgatacgct tctggttccc gtctctttac aagggcgtgt
     7561 aacaggtaac agcggcgttg atattttcgc ctaacgtcag aggtagcacc gtaatccgcg
     7621 tcttttcccc gctttgttgc gctcaagacg caggataatt agccgataag cagtagcgac
     7681 acaggaagac cgcaacacat gactgattac gcgataagca agaaaagcaa gcgatcgctt
     7741 tggatcccga ttctggtatt cattaccctc gcggcctgtg ccagcgcagg ttacagctac
     7801 tggcattcgc atcaggttgc cgctgacgac aaagcgcagc aacgcgtcgt gccctccccg
     7861 gtcttctatg cgctggatac ctttacggtc aatttgggcg atgcggatcg cgtgctttat
     7921 atcggcataa ccctgcgact gaaagatgaa gctacccgtt cgcggctgag tgagtatttg
     7981 ccggaagtcc gtagccgttt gctgttactg ttttcgcgtc aggatgctgc cgtactggcg
     8041 acagaagaag gcaagaaaaa cctgattgcc gagattaaaa ccacactttc caccccgctt
     8101 gttgccgggc aaccgaaaca ggatgtcacc gacgtgctgt ataccgcttt tattctgcga
     8161 taacgacatg ggcgatagta ttctttctca agctgaaatt gatgcgctgt tgaatggcga
     8221 cagcgaagtc aaagacgaac cgacagccag tgttagcggc gaaagtgaca ttcgtccgta
     8281 cgatccgaat acccaacgac gggttgtgcg cgaacgtttg caggcgctgg aaatcattaa
     8341 tgagcgcttt gctcgccatt ttcgtatggg gctgttcaac ctgctgcgcc gtagcccgga
     8401 tattaccgtc ggggccatcc gcattcagcc gtaccatgaa tttgcccgca acctgccggt
     8461 gccgaccaac ctgaacctta ttcatctgaa accgctgcgc ggcacagggc tggtggtgtt
     8521 ctcgccgagt ctggtgttta tcgccgtgga taacctgttt ggcggcgatg gacgcttccc
     8581 gaccaaagtg gaaggccgcg agtttaccca taccgaacag cgcgtcatca accgcatgtt
     8641 gaaactggcg cttgaaggct atagcgacgc atggaaggcg attaatccgc tggaagttga
     8701 gtacgtgcgt tcggaaatgc aggtgaaatt taccaatatc accacctcgc caaacgacat
     8761 tgtggttaac acgccgttcc atgtggagat tggcaacctg accggcgaat ttaatatctg
     8821 cctgccattc agcatgatcg agccgctacg ggaattgttg gttaacccgc cgctggaaaa
     8881 ctcgcgtaat gaagatcaga actggcgcga taacctggtg cgccaggtgc agcattcaca
     8941 gctggaactg gtggccaact ttgccgatat ctcgctacgc ctgtcgcaga ttttaaaact
     9001 gaaacccggc gacgtcctgc cgatagaaaa acccgatcgc atcatcgccc atgttgacgg
     9061 cgtcccggtt ctgaccagtc agtacggcac tctcaacggt cagtatgcgt tacggataga
     9121 acatttgatt aacccgattt taaattctct gaacgaggaa cagcccaaat gagtgacatg
     9181 aataatccgg ccgatgacaa caacggcgca atggacgatc tgtgggctga agcgttgagc
     9241 gagcaaaaat caaccagcga aaaaagcgct gccgacgcgg tgttccagca atttggcggt
     9301 ggtgatgtca gcggggcgtt gcaggatatc gacctgatta tggatattcc ggtcaagctg
     9361 accgtcgagc tgggccgtac gcggatgacc atcaaagagc tgttgcgtct gacgcaaggg
     9421 tcagtcgtgg cgctggacgg tctggcgggc gaaccgctgg atattctgat caacggttat
     9481 ttaatcgccc agggcgaagt ggtggttgtt gccgataaat atggcgtgcg gatcaccgat
     9541 atcattaccc cgtccgagcg gatgcgccgc ctgagccgtt agtgatgaat aaccatgcta
     9601 ctgtgcaatc ttccgcgccg gtttctgctg agccactgct gcaggtgagc ggcgcactca
     9661 tcgccattat tgccctgatc ctcgctgctg cctggctggt aaaacggctg ggatttgccc
     9721 ctaaacgcac tggcgttaac ggtctgaaaa ttagcgccag tgcttcgctg ggcgcgcgtg
     9781 aacgggtcgt ggtggtcgat gtggaagatg cacggctggt gctcggcgtt accgcagggc
     9841 aaatcaatct gctgcataaa cttccccctt cagcaccaac ggaagagata ccgcagaccg
     9901 attttcagtc ggtcatgaaa aatttgctta agcgtggcgg gagatcctga tgcgtcgttt
     9961 gttttctgtc gcaccggtcc ttctctggct gattacgccc ctcgccttcg cgcaactgcc
    10021 gggtatcacc agccagccgc tacctggcgg tggacaaagc tggtcgctcc cggtgcagac
    10081 gctggtgttc atcacctcgt tgacgttcat tccagcgatt ttactgatga tgaccagttt
    10141 cacccgcatc attattgttt ttggtttatt gcgtaacgcg ctgggaacgc cctccgcgcc
    10201 acctaaccag gtattgctgg ggctggcact gtttttgacc ttttttatta tgtcgccagt
    10261 gatcgacaaa atttatgtcg atgcgtacca gccattcagc gaagagaaaa tctcaatgca
    10321 ggaggcgctg gaaaaagggg cgcagccgct gcgtgaattt atgctgcgtc agacccgcga
    10381 ggcggattta gggttgtttg ccagactggc gaataccggc ccgttgcagg gacctgaagc
    10441 tgtgccgatg cgcattttgc tcccggccta cgtgaccagc gagttgaaaa ccgcatttca
    10501 gataggcttc acgattttca tccctttttt gattatcgac ctggtgattg ccagcgtgtt
    10561 gatggcattg gggatgatga tggttccccc cgccaccatt gctctgccct ttaaactgat
    10621 gctgtttgta ctggtggatg gctggcaatt gctggtcggt tcgctggcgc agagctttta
    10681 cagctagaga ggcaaaatga cacctgaatc ggtcatgatg atgggaactg aagcgatgaa
    10741 agtcgcgctg gcactggcag ccccgctgtt gctggtggcg ctggtcacgg gccttatcat
    10801 cagtattttg caggcagcca cgcagattaa cgaaatgacg ctgtcgttta ttccgaaaat
    10861 catcgccgta tttatcgcca ttattattgc cggaccgtgg atgctcaatc agttgctgga
    10921 ttacgtccgc accttgttca ctaacctgcc gtatatcatc gggtagcccg tacaatgatg
    10981 caggtgacaa gcgatcaatg gctttcctgg ttaagcctgt acttctggcc gttactgcgc
    11041 gtgctggcgc tcatttctac cgcgccaatt ctgagcgaac gcagcgtgcc gaaacgggta
    11101 aaactgggtc tggcaatgat gatcacgttc gccattgccc catcattacc tgccaacgat
    11161 gttcctgttt tttcgttctt tgctctgtgg ctggccgtgc agcagatcct gatcggcatt
    11221 gcgcttggtt ttaccatgca atttgccttt gccgctgtgc gaaccgctgg cgaaattatc
    11281 ggtctgcaaa tggggctgtc atttgcgaca tttgtcgatc ctggcagcca tctcaatatg
    11341 cccgttttag cgcgcatcat ggatatgctg gcattactgc tgttcctgac atttaacggt
    11401 catttatggt tgatttcact gctggtcgat acctttcaca ccctgccgat tggtagcgaa
    11461 ccgttgaaca gcaatgcgtt tctggcactc accaaagcag ggagtttgat tttccttaac
    11521 gggctgatgc tggcgttacc gctcattact ctgttgctga ccctgaatct ggcattaggt
    11581 ttactgaatc gtatggcccc gcaattatcc atttttgtta ttggatttcc attaactctg
    11641 actgtcggca tctctttaat ggcggcatta atgccgttaa ttgcgccttt ttgcgaacat
    11701 ttattcagtg agatgtttaa tttgctggct gatattatta gtgaattacc attaatataa
    11761 ttccgtaacg tttatcatgc taccctaagg attatccgaa aaataatatc tgcgaacatc
    11821 ttccaggata ctcctgtagc gaaatatttg ttttaagctc actcacatat cgcaacattt
    11881 actttacttt aagacaattc caggcaaatt atacaacact ttacgggata gtaagtccgc
    11941 ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt gttccgttta gtcatgatga
    12001 aatattcagg taaggggaat tatcgttacg cattgagtga gggtatgcca tgtcaacgat
    12061 tattatggat ttatgtagtt acacccgact aggtttaacc gggtatctgt tgagtagagg
    12121 ggttaaaaaa agagaaatca acgacattga aaccgttgat gaccttgcca tagcttgtga
    12181 ttcacagcgc ccttcagtgg tgtttattaa tgaggactgt ttcatccacg atgcttctaa
    12241 cagtcagcgc atcaagcaca tcattaatca acatcccaat acgttattta tcgtttttat
    12301 ggcaattgcc aatgttcatt ttgatgaata tctattggtc agaaaaaatt tattgatcag
    12361 ttctaaatcg attaaaccgg aatctctcga cgatatcctt ggcgatattc tgaaaaaaga
    12421 gacaacgata acctcgtttt taaatatgcc gacgttatca ttgagccgaa ccgaatcgag
    12481 tatgttgcga atgtggatgg caggtcaggg aaccattcaa atctccgacc aaatgaatat
    12541 caaagccaag accgtttcat cgcataaagg taatattaaa cgtaagatca aaacgcataa
    12601 taaacaggtt atctaccatg tcgtccgact gacggataat gtgactaatg gtatttttgt
    12661 caacatgcgc taacacattc tgactggtgg tttcccacca gtcaggctga ataagattac
    12721 tctgctttct caacaaagat accgtcctga tgccctgctt cattaaagaa ccagatgccg
    12781 agcgggtagt cttccagcga aaccaggtac attgtgcctt cactaaactc ctcaactgcc
    12841 agtaccacgc caggacgacg cggaccgcca tccgttttga ctgttacccg atcattcacc
    12901 ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac aatttcgttt tcctggcagc
    12961 gccaggcgcg cgcgagtgct gattttctcg acggtctata cttaagagat gccaggcgga
    13021 cttaacgact ggcggcaaca acagagtaac ggttgcgagg aaagatgatg aaaaccgcaa
    13081 aagagtacag cgataccgca aaacgtgagg tcagcgtcga tgtcgatgcc ctgctggcgg
    13141 cgatcaatga aattagcgaa agcgaagttc atcgcagcca gaacgattct gaacacgtta
    13201 gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc gaactggata
    13261 ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa atcccgaccc
    13321 tgagggggtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc gttacaccag
    13381 gaaatctgat gtgttcatca ccttatctgc aatttttttc gctgacaaga aaatattcgc
    13441 gctatgaatg attaacgcta cttatgaata gaaatgtgac gcgcgtcacc ccttggtggc
    13501 tctggtgtca acgggatcgc tcccgaaaaa atcatctttg attatttgtg ctgttttgag
    13561 aatcatcctg gtaaggggag tactgcgccc atgttttcaa ttcaacaacc actactggtt
    13621 tttagcgatc ttgatggcac cctgctggac agtcatagtt atgactggca accggcagcc
    13681 ccctggctca gccgtttaca cgaagccaat attcccgtca ttctctgtag cagtaaaact
    13741 tcagcggaaa tgctgtactt gcaaaaaatg ttggggctac aaggcttacc gctgattgca
    13801 gagaatggcg cagtgatcca gcttgctgaa caatggcagg atatagacgg ttttccacgc
    13861 atcatctcag gtattagcca tggcgaaatc tgccaggttt taaatacgct acgcgagaaa
    13921 gaacatttta aattcacgac ttttgatgat gtcgacgatg caaccattgc cgaatggacg
    13981 ggattaagcc gtagccaggc ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc
    14041 tggcgcgaca gtgacgagcg tatggcacaa tttatcgctc gtctgaacga actgggcttg
    14101 cagtttatgc agggtgcgcg cttctggcac gtactggatg cctctgccgg aaaagatcag
    14161 gctgccaact ggattatcgc gacctatcaa caattgtcag gcagacgccc aaccacactt
    14221 ggcctgggcg atgggccaaa cgatgcgcct ttactggagg taatggatta cgcggtgatt
    14281 gtgaaagggc taaaccgtga aggggtccat ctgcatgatg aggatccggc ccgcgtctgg
    14341 cgaacgcagc gtgaaggacc ggaaggctgg cgtgaagggc tggaccattt tttctccgcc
    14401 cgttaagcgt tatcgctcgc gaatacccga ttacgcccgg cctgttttgc gaggtaaagc
    14461 cgacggtcgg ccaatgactg gagttgttca aaatcataat caccggtttc ctcgctgcta
    14521 cttaccccca gcgaggcact gatgcgtatc gttgtactct tggcgatcag catctctttt
    14581 tcatttaact tcaggcgaat acgttctgcg acctccgcag cctgcgtcag attcgcgcct
    14641 ggcagaatca cacaaaactc ctcaccaccg acccgcccgg caatgtcctg cgcacgtaag
    14701 gaactgctaa ttaatccggc agcatgagaa agaacacggt cgcccgcctg atgaccaaag
    14761 cggtcattaa tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg
    14821 tgcgtgtgac acaatttagc gagcggacag gctttttcga acaatgcgcc acggttatat
    14881 aaacgcgtta aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag aacatacatg
    14941 ttgctgacca tccggcgaat cacataccag gagaggagta acatgctggt aaagagcgcc
    15001 cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc cgcgcacgcc ttcgcttagc
    15061 gtatggacgc gcaccagcac accgtcgaaa tggtccagac gttcccagct aacatagcga
    15121 ctgtcaatac gaatgccgcc ccgcgtgtca tgttccattg cctgcgccag caaggccagt
    15181 tcacgaggat caaaaatatt ccctgttggg tggtcaggat ttgaagaggt caaaaatctc
    15241 agcttactgt catagagctg atactcacca tcgaggtttt tatcgatggc gtttcttaaa
    15301 aattgctgca tagtacgcac gggaatactc atccccagca cgccatacca gtagttatta
    15361 ctgtctaccg gaacactgac ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc
    15421 gaagtgaacc agcgtaccgc gcggtgacga ttttctcgtt gtgaatggcc gataaaccag
    15481 ggttgggtga catagccgta ataacgcgtt ggtacattgc gcgtaaacaa ggtcggcagc
    15541 gtcgaaacgt aaaatccagc acgcgagaca tacaccgcct gttcaaccat cgacgaggag
    15601 ttgtgcgcca gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc attgtcgagg
    15661 ctttcatttt cgcgagacag gagattcccc tcgctgacta atgcatccga tacgccattg
    15721 actggcatgg tgcgccgtcg gttgagttcg atttgccagg cgtgctcgtc gcgatgctgt
    15781 tcgaactcgg ttacggcatt acgcagagag atgaaatcca gtggcgccac gagagcttcg
    15841 cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg cttatcgagc
    15901 gcgttggcta cgttttccag atgattacgc tggctggaga tataggcatc ttccagcacc
    15961 accacttcac gccaggtgag caaggtggaa aaaagcaata ccacgataaa gcagagatta
    16021 acgacatgac caggccccag gcggcgtgcg agttttttca accagctctg gttttccatt
    16081 tttgtctcgt gctgcaccct gacgactccc aacctgcttt tttatgattc tggcataacc
    16141 cggcgatagc gtaccgcaaa acagcgataa ttttataatg gtggcatgat aagacgcgac
    16201 tatcattcat tgcaggcatt gggacaccag gccggatgtg atccggcctg gtgttaatga
    16261 taccctttca aacttcttcc gcagaacgac tcccctcccc cggaacaaga tcggtttcat
    16321 gaaaagcttc ccgcttgacc ccgtaaccgt cataccaacg acactcaacc ataccgctgg
    16381 agtatccagt gacaatcatc cgcgggccgc cctctttaac cgtaacttcc tcactaacca
    16441 taaagctcat actcgcctcc ttttttcgag tgaaacttgt tcaccttagt tgaagatggc
    16501 gaattttgca taatagccag tgcgagtatt aatgtgcctg accgcgcatt ttcgctactg
    16561 ctttcactcc cagcaccacg atgccgccga tgataaatcc aagaatcaga tttaaaatag
    16621 tcggtaatat catcgccacc actgcacttt gctgcccggc gatatgttca atagcgtgat
    16681 gcagcggcgc aataccatgt accacaatcc cgccgccgac gaggaacatc gccagcgtgc
    16741 cgacaatcga tagcgctttc atcagccagg gcgcgataat caataaccct ttacctaatg
    16801 cctgcatcag cgcgctggat ttttccgcca gccaataccc caggtcatca atcttaacga
    16861 taacccctac cagaccgtag acgcccacgg tcactaccag cgcgatgcct gaaagcacca
    16921 gcacctgatt aagcaacggt gcctctgcca caatccccag cgtaatggcg acgatttccg
    16981 cagacaaaat aaaatcggta cgaatcgccc ctttaatttt gtccttttca aacttcagcg
    17041 gatcctgcgc cgccagcttc tccagacgct gctggctctg cgctggatct tctttatgtt
    17101 tacgcgcctc cagcatatgc agcactttct ctaccccttc aaagcagaga aacgcgccac
    17161 caatcatcaa cagtggcgta atcgcccacg ggataaacgc actgatgatc agcgccagcg
    17221 gcaccagaat cactttattg atcagcgatc ctttcgccac gccccagacc acgggaagtt
    17281 cccggttggc ccgcacgcct gaaacttgtt gcgcattgag cgataaatca tcccctaata
    17341 caccggcggt tttcttcgcc gccagtttgc ccatcacgga gatatcgtcc agcagtgtgg
    17401 cgatatcatc gagcaacgtc agtaaactac tcccggccaa aagcattccc tctcattttt
    17461 attgttgaag ggtgtaagta tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc
    17521 aacatttatt taacaatcaa gtgcaattat atctctcgcc agcagaatga ttttcacgtt
    17581 tactatgaac caccttttta tttccgccgt gagggattat gcgtttccgc cagttgttac
    17641 cgctttttgg cgcgctgttt gcgttgtata tcatttgggg ctcaacctat tttgtcattc
    17701 ggattggcgt ggaaagctgg cctccgttaa tgatggcggg cgttcgattc ctggcagccg
    17761 gtattttatt gctggcattt ttgctactgc gcggacacaa acttcccccg ctacgtccgc
    17821 tgctcaatgc cgcgctgatt ggcctgttat tgctggctgt cggtaatggc atggtgacgg
    17881 ttgccgaaca tcaaaatgtt ccttccggca tcgccgccgt agtggttgca accgtgcccc
    17941 tctttaccct gtgcttcagt cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg
    18001 gtatcgccat tgggcttgcc ggaatcatca tgctcaatag cggtggaaat ttaagcggca
    18061 atccgtgggg cgcgattctg attttaatcg gctcgattag ctgggcgttt ggctcagttt
    18121 atggctcgcg cattacctta cctgtaggga tgatggcggg tgcgattgag atgctggcgg
    18181 caggcgtggt gttaatgatc gcgtcgatga ttgcgggtga aaaactgacg gcgctccctt
    18241 ccctgtcagg cttccttgcg gtcggctatc tggcgctgtt tggttcgatt atcgccatta
    18301 acgcttatat gtatttaatc cgtaatgtca gtccggctct cgccaccagc tacgcctacg
    18361 ttaacccggt ggtcgcggtc ttgctgggta cgggactggg tggcgaaacg ctgtcgaaga
    18421 ttgaatggct ggcgctcggc gtaattgttt tcgcggtggt actggtcacg ttgggaaaat
    18481 atctcttccc ggccaaaccc gttgtcgcgc cagttattca ggatgcatca agcgagtaaa
    18541 tgaatcccct gcgtgtcgat ctgcgcgctg gcaccttcgc cgcagatcca ctcttccaga
    18601 cgctcggtaa gcgccgcatc cgtcagcttc tcgcgcccac gtaacgcaca ctcccagaca
    18661 atcagtacgc gccagccgag ctcctgcaag cgactgatat cgcggcgatc gcgttcaaca
    18721 tttttaccta tcttctccag ccagaactcg gttcgagtcg caggcacttt aaacagatag
    18781 cagtgatgat gatgccagaa gcagccgtgg gtaaatatca cgcagcgata ttcatcaacg
    18841 acaaaatccg ggcgtccggg cagactggcg tcctgaacgc gaaacgcaag gccctgcccg
    18901 gttaacaaac tggcgaggcg cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata
    18961 tttttgctgc gggtggcctt atcgtgaacg tcgaccatgt tgtgcctctt gctgacgcaa
    19021 cgccaccgcc tgtttgattt ttggctcaag cagttttgcc accgcggcaa agaccggcac
    19081 gaccaccgag ttaccgaact ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt
    19141 cgcttctccc ggcgcttcaa aacccattaa gcgcgcgcat tcacgaggtg ttaaccgacg
    19201 tggacgatgt tgctgattca gcggatcgtc aaagtctttc tcacccgtgg ccatatccca
    19261 gccgcggtcg attaaaattt ccgcgccatc tttgtaataa cgagcagaca gcgtacgggt
    19321 gacgctttgc ggattgttcg gataaaccat tccataaccg aagccgttac cgcgcgcctg
    19381 atgttttttc gcatatcgat agaggtactt ccacagcacc ggcgtcagga tatatttcgc
    19441 ctcaaccatc gggtccagca gctgcgccag cgtcactcgc tgtgcaggga aacattcgct
    19501 gatatcacgc agagtaaaat cggctttcag attcagatcg cgacgaaaac ccaccagcac
    19561 gatgcgttca cggtgctgcg gcaggaaatg tttaccatcg atgattttcg gatcgtccgg
    19621 cccgttatct tctgcatcag ccacgtcata gcccagttcg tccagcgtct gcatgatgat
    19681 gcggaacgtt tttcccttat cgtgactctt caggtttttg acgttttcga gcacaaacat
    19741 cgccggacga cgcgcgtcga taatgcgcac cacatcaaag aacagcgtgc cctgggtatc
    19801 gcaggcaaaa ccgtgcgccc gcccgagcga gttctttttc gatacgccag ccagcgaaaa
    19861 tggctgacaa gggaaaccgg ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg
    19921 ttccgccgcc gcctcatcac tcacgccttc ttgatggctg agggtgatgt cgcggatatc
    19981 ctcattaaaa tgatgcgtcg ccgggtcgca ataatggttg gctttataag tgcgtaccgc
    20041 atgtttgttc cattcgctgg taaacacgca ctgtccacca atcgattcaa aaccacgacg
    20101 gatgccgcca attccggcaa atagatcgat aaagcgaaac gcataatgcg gatgatgttc
    20161 cggtggtttg ggtaacaacg tttgcagatg ggcaaactct ttctcactta aacggtgcca
    20221 tgccgagtca ttcgccagcg cacgttttaa aattgccgcg ctccagtgat tctcacccac
    20281 accattaagc tgcgccacca gcgttttaac atcataaatt tgcagcagtt tctccagcat
    20341 tgcctgtgcg gcattcccgg tgctgtatga atcggttact gatatatttt cctgcataga
    20401 tttaaccggc catctgagat ggccgacagg ttaacataat tactgccgtt tagggccacg
    20461 cgctcagcca gcaggtgaaa acgcatctat caccttgtga tcgatacctt cattctcccc
    20521 cgccagttcg gcaccgagct tcgccataaa ctcgactaaa aattgcgcgt tgtgctgtgc
    20581 cagctgcttg cctcttgcgg tttgcatggt ttgcggtaat ttcagtaact ttgtctggaa
    20641 atgatccagc gcgtagcgtt tatcatcaag cgggcgatgc tgtgcgaacg gatcttcgcc
    20701 atcaaacagc gccaagccca atgcccctga aacggcaaat acgcgcgcca ggccaatcgc
    20761 ccccagggct tccagccgat ccgcatcctg gacgatttta gcttccgttg ttaagggggc
    20821 gatttgcgcg ctgaaactgt gagcggcaat ggcatgacaa acggcctcaa ttttctccgc
    20881 cggaaattgc acgaactctt cacggagcag gcgacgcgtc tcttctgccg ccaggattga
    20941 agaacgctgc cgttgcggat gatttttcgc caggctcacg atatcgtgaa aataacaggc
    21001 ggttaaaatc accagcatat cgacatcgtc atctgccgcc agtttctgtg ccgtagccca
    21061 gacacggcga aaatgacaca ggtcatgcgc cgcgtcctga tgttgatgat ggtttttcaa
    21121 ccagttctcg aactgtgcct gccagtgttg taactccata cgcgctccgt tgccaaagag
    21181 tcgcaacctt agcagttttt attcatagcg tctgcaacgt gcgggacgag gtcgggtgta
    21241 ccatgcaatg ccgcctaaca aggcaccaac cagggccata aacagaaatc cgaccaatgc
    21301 tcctaaccac ttccccgcgg tagatcctaa atcactttca aatcccgaca cgggggcatc
    21361 ggggatccgg ctcatcaccc agatgtatcg cgccattgcc cagacgaaat agccacacca
    21421 tgtatagaaa gcccacaaag ccaatttgcc gccagggctg cgagagagtc tctcttccat
    21481 ctttgtcgaa ccgtattcca ttttgagata acccggaaaa ttcagactta gaactattat
    21541 gtgataaaaa tcacattttt ccacattgga tgagccagt
//
LOCUS       DAZFRG010000064        21069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFRG010000064 DAZFRG010000000
VERSION     DAZFRG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.064"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            495..4955
                     /gene="gltB"
                     /locus_tag="ACHKUC_001874"
     CDS             495..4955
                     /gene="gltB"
                     /locus_tag="ACHKUC_001874"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO6618138.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEK
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4968..6386
                     /gene="gltD"
                     /locus_tag="ACHKUC_001875"
     CDS             4968..6386
                     /gene="gltD"
                     /locus_tag="ACHKUC_001875"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO6618139.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNIQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(6454..6918)
                     /gene="nanQ"
                     /locus_tag="ACHKUC_001876"
     CDS             complement(6454..6918)
                     /gene="nanQ"
                     /locus_tag="ACHKUC_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO6618140.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKIKADLMA"
     gene            complement(6915..7790)
                     /gene="nanK"
                     /locus_tag="ACHKUC_001877"
     CDS             complement(6915..7790)
                     /gene="nanK"
                     /locus_tag="ACHKUC_001877"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6618141.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     TGLLGAALLAQGEIL"
     gene            complement(7787..8476)
                     /gene="nanE"
                     /locus_tag="ACHKUC_001878"
     CDS             complement(7787..8476)
                     /gene="nanE"
                     /locus_tag="ACHKUC_001878"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6618142.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(8524..10014)
                     /gene="nanT"
                     /locus_tag="ACHKUC_001879"
     CDS             complement(8524..10014)
                     /gene="nanT"
                     /locus_tag="ACHKUC_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO6618143.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNNLVKTKS"
     gene            complement(10123..11016)
                     /gene="nanA"
                     /locus_tag="ACHKUC_001880"
     CDS             complement(10123..11016)
                     /gene="nanA"
                     /locus_tag="ACHKUC_001880"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6618144.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(11138..11929)
                     /gene="nanR"
                     /locus_tag="ACHKUC_001881"
     CDS             complement(11138..11929)
                     /gene="nanR"
                     /locus_tag="ACHKUC_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6618145.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(12037..12534)
                     /gene="sspB"
                     /locus_tag="ACHKUC_001882"
     CDS             complement(12037..12534)
                     /gene="sspB"
                     /locus_tag="ACHKUC_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO6618146.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(12540..13178)
                     /gene="sspA"
                     /locus_tag="ACHKUC_001883"
     CDS             complement(12540..13178)
                     /gene="sspA"
                     /locus_tag="ACHKUC_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO6618147.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(13572..13964)
                     /gene="rpsI"
                     /locus_tag="ACHKUC_001884"
     CDS             complement(13572..13964)
                     /gene="rpsI"
                     /locus_tag="ACHKUC_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO6618148.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(13980..14408)
                     /gene="rplM"
                     /locus_tag="ACHKUC_001885"
     CDS             complement(13980..14408)
                     /gene="rplM"
                     /locus_tag="ACHKUC_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO6618149.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(14628..15755)
                     /gene="zapE"
                     /locus_tag="ACHKUC_001886"
     CDS             complement(14628..15755)
                     /gene="zapE"
                     /locus_tag="ACHKUC_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO6618150.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRFFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            15949..16347
                     /gene="zapG"
                     /locus_tag="ACHKUC_001887"
     CDS             15949..16347
                     /gene="zapG"
                     /locus_tag="ACHKUC_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO6618151.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            16501..17868
                     /gene="degQ"
                     /locus_tag="ACHKUC_001888"
     CDS             16501..17868
                     /gene="degQ"
                     /locus_tag="ACHKUC_001888"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO6618152.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTTSQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            17958..19025
                     /gene="degS"
                     /locus_tag="ACHKUC_001889"
     CDS             17958..19025
                     /gene="degS"
                     /locus_tag="ACHKUC_001889"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO6618153.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(19088..20026)
                     /gene="mdh"
                     /locus_tag="ACHKUC_001890"
     CDS             complement(19088..20026)
                     /gene="mdh"
                     /locus_tag="ACHKUC_001890"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6618154.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            20461..20931
                     /gene="argR"
                     /locus_tag="ACHKUC_001891"
     CDS             20461..20931
                     /gene="argR"
                     /locus_tag="ACHKUC_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO6618155.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT         5122 a         5676 c         5480 g         4791 t
ORIGIN      
        1 ctccttatgg attttaggca aaaacagtag catgaaacgt tattaccaat taaggcagta
       61 taaaatgctg gctttctcgt caattcaagg caggataagg gttaacacgc ctttatgaca
      121 gtcaggaact ggctgtttct ctaacgacgc cccttttagc cttaaataga aaatccaatt
      181 taatttcaga taattaataa agaattttgc gctaaagcac atttctgtac caataagctt
      241 gccatttgac ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat
      301 gagcagcctg ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg
      361 atgcgaaaag gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc
      421 gccacgctct tactgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg
      481 gggaggttca cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc
      541 tgatcgccca catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac
      601 tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg
      661 gcttgctgtt acaaaaaccg gatcgttttt ttcgcatcgt tgcgcaggag cgcggctggc
      721 gtttagcaaa aaactacgcc gtcgggatgc tcttcctgaa taaagatcct gaactcgccg
      781 ctgccgcacg ccgcatcgtt gaagaagagc tgcaacgcga aacgttgtcg attgtgggct
      841 ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac
      901 gcattgagca aatttttgtg aacgccccgg caggctggcg tccgcgcgat atggagcgcc
      961 gtctgtttat cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg
     1021 tctgtagcct gtcgaatctg gtcaatattt ataaaggtct gtgtatgccg gcggatctgc
     1081 cgcgcttcta cctggacctc gctgacctgc gtctggaatc ggccatctgc ctgtttcacc
     1141 agcgcttttc cactaacacc gtaccgcgct ggccgctggc gcagccattc cgctacctcg
     1201 cgcataacgg tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct
     1261 ataaattcca gacgccgctg atccctgacc tgcacgacgc cgcgccgttc gtcaacgaaa
     1321 ccggctctga ctccagttcg atggataaca tgctggaact cctgctggca ggcgggatgg
     1381 atatcatccg tgccatgcgt ctgttagtgc caccagcctg gcagaacaac ccggatatgg
     1441 acccggaact gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggtc
     1501 cggcaggcat cgtgatgtct gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc
     1561 tgcgtccggc acgctacgtc atcaccaaag ataaactgat cacctgcgcc tctgaagtcg
     1621 gtatctggga ttatcagcct gacgaagtgg tcgaaaaagg ccgcgtaggg ccaggcgaac
     1681 tgatggtgat cgacactcgc agtgggcgca ttctgcactc ggcagaaacc gatgatgacc
     1741 tgaaaagccg ccatccctat aaagagtgga tggagaaaaa cgtccgccgt ctggtaccgt
     1801 ttgaagatct gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca
     1861 gctaccagaa acagtttaac tacagcgcgg aagagctgga ttcggtgatc cgcgtactgg
     1921 gtgaaaacgg tcaggaagcg gtcggttcga tgggcgacga caccccattc gccgtgctct
     1981 ccagtcagcc gcgcattatt tacgactact tccgccagca atttgcccag gtgactaacc
     2041 cgccaatcga cccgctgcgc gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg
     2101 aaatgaacgt cttctgcgaa gcagaaggcc aggcgcaccg tttaagcttt aaatcgccga
     2161 ttctgctcta ttccgatttc aaacagctca cgacgatgaa agaggatcac taccgcgcag
     2221 atacgctgga tatcaccttt gacgtcacta aaaccacgct cgaagcgacg gtgaaagaac
     2281 tgtgtgacaa agccgaaaaa atggtacgta gcggcaccgt gctgctggtg ctctccgacc
     2341 ggaatatcgc taaagatcgc ctgccggttc cggctccgat ggcggttggc gcgatccaga
     2401 cccgtctggt cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg
     2461 cccgcgatcc gcaccatttc gccgtgttgc tgggtttcgg cgcgacggct atttatccgt
     2521 accttgccta tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc aaagattatc
     2581 gtaccgtgat gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca
     2641 aaatgggcat ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc
     2701 tgcacgatga tgtcgtgggc ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa
     2761 gctttgaaga cttccagcag gatctgctga acctgtcgaa acgtgcctgg ctggcgcgta
     2821 agcccatcag ccagggcggc ctgctgaaat atgtgcacgg cggcgaatac cacgcctaca
     2881 acccggacgt ggtgcgcacg ctgcaacagg cggtacagag cggcgagtat agcgactatc
     2941 aggaatacgc gaagctggtt aatgagcgtc cggcaaccac gctacgcgat ctgctggcaa
     3001 ttacgccggg tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta
     3061 aacgcttcga taccgctgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc
     3121 tggctgaagc gatgaacagc atcggcggca actctaactc cggtgaaggc ggcgaagatc
     3181 cggcgcgcta cggcactaac aaagtgtcgc gcatcaagca ggtggcttcc ggtcgcttcg
     3241 gtgttactcc ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg
     3301 cgaaaccggg tgaaggcggt cagttaccgg gcgataaagt cacgccttac attgccaaac
     3361 tgcgctattc ggtgcctgga gtgacgctga tctccccgcc gccgcaccac gatatctact
     3421 ctatcgagga cttagcgcag ctcattttcg acctcaagca ggttaacccg aaagcgatga
     3481 tctccgtgaa gctggtttcc gaaccgggcg ttggcaccat cgcgactggc gtggcaaaag
     3541 cttatgccga tttaatcacc atcgcaggct atgacggcgg caccggcgca agtccgcttt
     3601 catcggtgaa atacgcaggc tgtccgtggg agctggggct tgttgaaacc caacaggcgc
     3661 tggttgccaa tggtctgcgt cataagatcc gtttgcaggt tgatggcggc ctgaaaaccg
     3721 gcgttgatat catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc
     3781 cgatggtggc gctcggctgt aaatatctgc gtatttgcca cctgaacaac tgcgccacgg
     3841 gtgtagcaac tcaggatgac aaactgcgta agaatcacta tcacggcctg ccgttcaagg
     3901 tgactaatta ctttgagttt atcgcacgtg aaacccgcga gctgatggca cagctggggg
     3961 taacgcgtct ggtggatctg attggtcgca ccgacctgct gaaagagctg gacggtttca
     4021 ccgccaaaca gcagaaactg gcgctgtcga agctgctgga aactgccgag ccacatgcag
     4081 gtaaggcact ctactgcacc gaaaaaaacc cgccgtttga taacggccta ctgaacgcgc
     4141 agttgctgca acaggcaaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg
     4201 atatccgcaa caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc
     4261 acggcgatca gggtctggca gccgatccaa tcaaagcgta cttcaacggc actgcaggcc
     4321 agagcttcgg cgtgtggaac gcgggcggcg tggaactgta cctgaccggt gatgccaacg
     4381 actatgttgg taaaggcatg gcgggtggct taatcgccat tcgtcctccg gttggttccg
     4441 ccttccgcag ccatgaagca agtattatcg gcaacacctg cctgtatggc gcgaccggtg
     4501 gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcgtaac tccggtgcta
     4561 tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtatcgtct
     4621 gtattctggg taaaaccggc gttaacttcg gcgcaggcat gactggcggc ttcgcttacg
     4681 ttctcgatga aagcggcgat ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa
     4741 gcgttgacga tctggcgatc catgaagagc atctgcgtgg tcttatcacc gagcatgttc
     4801 agcataccgg ttcccagcgt ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta
     4861 aatttgcgct ggttaaaccg aagtccagtg atgtaaaagc actgctgggt caccgtagtc
     4921 gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg
     4981 tttatcaatt tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgca
     5041 aaattgagtt tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg
     5101 accgctgcct gtcgtgcggc aacccatact gcgagtggaa atgcccggta cacaactaca
     5161 tcccgaactg gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc
     5221 atcagaccaa taccctgccg gaagtgtgcg gacgtgtctg cccgcaagac cgtctgtgcg
     5281 aaggttcctg caccctgaac gatgagtttg gcgcagtgac catcggcaac attgagcgct
     5341 atatcaacga taaagcgttc gagatgggct ggcgtccgga tatgtccggc gtgaaacaaa
     5401 ccggtaaaaa agtggcgatt atcggcgctg gcccggcggg tctggcgtgc gcggatgtcc
     5461 tgacgcgcaa cggcgtaaaa gcggtcgtct tcgaccgtca cccggaaatt ggcggattgc
     5521 tgaccttcgg tattccggcc ttcaagttag aaaaagaggt aatgacgcgc cgccgtgaaa
     5581 tcttcaccgg tatgggtatt gaattcaaac tcaataccga agtgggccgc gacgtacagc
     5641 tggacgacct gctgagtgat tacgatgccg tgttccttgg cgtcgggact tatcagtcaa
     5701 tgcgcggcgg gttggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta
     5761 tcgccaacac caaacagtta atgggctttg gtgaaaccaa cgacgaaccg ttcgtcagca
     5821 tggaaggtaa acgcgtggta gtacttggcg gtggcgatac cgcgatggac tgcgtgcgca
     5881 cgtctgtacg ccagggtgca aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca
     5941 tgccaggttc ccgccgcgaa gtgaaaaacg cgcgggaaga aggtgtagag ttcaaattca
     6001 acatccagcc actggggatt gaagtgaacg gtaacggcaa agtcagcggc gtgaaaatgg
     6061 tgcgcacaga aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgtggcag
     6121 ggtcagaaca tatcgttccg gcagatgcgg tgatcatggc gtttggtttc cgtccacaca
     6181 acatggaatg gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgccc
     6241 cggaaggcag cgacaacgcc ttccagacca gcaacccgaa aatctttgct ggcggcgata
     6301 tcgtccgtgg ttccgatctg gtcgttaccg ccattgccga aggtcgcaaa gcggcagacg
     6361 gcattatgaa ctggctggaa gtttaagcga ggtaacaata aattcgccag acagtgtctg
     6421 gcgaatttat aaaacgagct atccaactaa attttaagcc attaaatcag ccttaatctt
     6481 cactaccacc tttttaatct caccaggttc gccaacaacg catcccggtt tatgcggttc
     6541 tcctggcata aacacggcga acattcccgg tttcaggatg atggcttgct cgttttcaat
     6601 ggcgctgcaa agctgataat catcttcatg gtggaactct tcacactgac gcgcagtgcc
     6661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
     6721 ttcgtgcaat tcggcttttt tctcaacggg cgattgagta ttaaatgtca tgacattcat
     6781 aaagatattg tcgccctgta attcgtaacg gccaggcgct ttttcttgcg gtctggcagc
     6841 taatgccaac gttaacgcgt cctgtaacgc aggatgtaat ccggcagacg gtaatgactg
     6901 tacttcaccc atcatcataa tatttctccc tgggccaaca gcgcagcccc aagtaaacct
     6961 gtatcgtggc ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     7021 agatacgttt ccaccagcgc cagataccct tctgccagac caacgctgcc accgaccacc
     7081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     7141 gagcggtgaa tcagttgctg cgcctgctca tcaccctgcc cggcgcgcgt gaaaatagtt
     7201 ttcgcattcg caccagccaa ctccccctgc gctgccgctg caatgccgcg accagaagca
     7261 atcgcttcca cgcaacctgt gcgtccacag ccgcagactg gaccatgcgg atcggcaagc
     7321 gtatgcccga tatgccccgc cagaccgcca gggccggtac gcagtttacc gccgctcact
     7381 acaccgccgc caacgccggt ggaaacagtg ataaacacca tatcggttat atcgccatcc
     7441 agcgcctgat actccgccca tgctgcggcc tgcgcatcat taatggcaat ggtcggcaaa
     7501 ttggtaagtt gctccagcgt tttgactaac ggaaagtgta acaatccacc gagattatgc
     7561 ggattcagcg ccagcaagct gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     7621 tgcgcatgtg cttgcaacgg agagactaat gcggataagg catcacgcag cgcctgcgga
     7681 gtctggctgg caggcgtagg aagttctcgg cgatcgcgga tctgcccgtc agcgccaatc
     7741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtgtggtcat agcaccgcct
     7801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     7861 ccgtcaccgc ccacgcgccg tggcgcatcg catccgccgc ctgagcaggc gtgttgtaac
     7921 gcccttcggc aatcacccgg catccagcgt cactcaacgt tttcaccagc gccagatccg
     7981 gctcttctgg cgtttcaggc gtagtatagc cagaaagcgt agtgccaata atttcggctc
     8041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     8101 cgtgatggtg aatacgtgcc agcagcgttt caactggcac cggacgcggt cggtcggtgc
     8161 cgtcaatcgc gataatgtcc gcgcccgcct gcgccagcgc atcaacatct tcaatatagg
     8221 ccgtgatgcg taccggagaa tcctccagat cgcgcttcac aatcccaata atcggcacgc
     8281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc aatacgaatg gcgaccgcgc
     8341 ccgcctgctc tgccgctaat gccatggcag cgacgatttc gggtttatcg agcgggctgt
     8401 ccggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     8461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacagaga ccgggcaaca
     8521 ggattaactt ttggttttga ctaaattgtt tttggcgctg ccaaatggca cggcaccgct
     8581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     8641 aacacgagaa ggcatatcca gaccaatcag caggatcacc acaaatgtca gactgaacga
     8701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     8761 ggccagtgca ccgcccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     8821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     8881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     8941 taccggaata atcagcagct gcgaggccag cagactacaa acgtatgctt tgcgggttcc
     9001 cagccagtca ccgaggaagc ccccgacgca gcaccccacc gccgcgccaa agccactaaa
     9061 gaacagcaca ttggcgacag tatgcgggtc ataagccaga tcggttttca gatacgttgg
     9121 cagcagcgcc tgaatcggcc atgagtagag gaaagcgaac aacacgacca ccatcagcat
     9181 tacgcccgtt ggccagcgtt tgcccgtact ctgcaccata aagctgataa aaattgcggc
     9241 gcataacagc ccaagaacag cgacgatcgc ggcattttgc aggttaccgg cgaagcagaa
     9301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     9361 gtagagaata tccaccattg tgcgtaccgg tgctttaccc ccgtgtttct ctttccagtc
     9421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     9481 aaagaacagc gcacgccagc cccagaccgg aaccaccaga ctatagacct gagcggcaac
     9541 gacggccccc actgagaagc ctgaaatcag aaaaccactg gctttgttac gcagatgctt
     9601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     9661 aatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     9721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     9781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     9841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     9901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gctgcggaaa atgcccgcca
     9961 ttgtgcgcgg ttaagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
    10021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
    10081 ttatttcccc tcacccggta ggggcgagcg aggggaaaca actcacccgc gctcttgcat
    10141 caactgctgg gccagcgcct tcagttctgg cagatatttt tcatctaccg gaccaaacgg
    10201 tttgcggcac agcggcacag aaacgacatc catataatgc aggacagttt tcaggccgcg
    10261 gaatacgccc gttttgatca gtaaatcaat gactttgttg cactcagttt gcagtttctg
    10321 cgcggtctgg atatcgcctt ctttcagcgc cttcacgatc ccctgataac gccagcccat
    10381 gatgttgtaa gtgctgccga taccgccgtc tgcgcccgcc agaagaccag aggcgaagat
    10441 ttcgtcgtaa ccgttataga gcaccagatc aggatgttca cgacggatct gctccatctg
    10501 atagagatcg ccagaggtct gtttcagcgc acctacacca ggcagcgtaa ccagggtgtt
    10561 gatctgatcc agggtcagtt ttaccccgct cagggctgga atgttgtaca ccaccatcgg
    10621 caacccatcc gccgaatcaa taattgcccg atagtgatcg cagtgttctt caaagctgaa
    10681 aggatagtag aacggcgtga cggcggagac ggcatcgaag ccataacgtt tagccgatgc
    10741 cgcaagttgt tggctttcgg cggtgctgac gcaaccgacg tgggcgatga gtttaatctt
    10801 acctttcgcc tcttcggcga cgatttccag tacctgttcg cgctcggaaa ggctttgtac
    10861 aaaggcttcg cctgtcgaac cacccacgta caaaccgtcg atgccctgct gaatattgaa
    10921 ctgaaccagg cggcgcagac tcgctttatc cagtgcttgt tgttggtcaa aaggagtcag
    10981 gagtgcagcc attacgccac gtaaattcgt tgccataaat acctctgaag tgatgcttgt
    11041 ctgataaacg atataccttt atacctgtta taccagatca attaagcaac accccataca
    11101 gaaagcttat aatgcgatct gcttcactaa agtggcatta tttctttttg ttggtggtct
    11161 gaccgaaggc gtgccaggta gcagagacgc tgttgagatg cgattgcaac gcgcgatcgg
    11221 cttcgtcagg atcatgacgg cggatcgcgt caacgatcgc aatatgctgt tgatagctaa
    11281 cgttgttatg ctcatgcaga gcctgatcgg ccacggttgg acgtgcggcg attagccagt
    11341 cgagcagggc aacgtggatc gccatgaaga ttgggttacc agggatctcc gccagaacgc
    11401 ggtggaaatc aacgtctgag cgaatgaatg ccgcgttgtt atccagcgac tgactgttga
    11461 tttccagtgc ttttgccagc aaatcgattt gctcatcggt ggcgtgttca gccgcatagc
    11521 gcaccaggct ggattcaaag aacagacgta attgttcgaa atgggcaatc ccaccaggat
    11581 gagaaaggaa atctttcgcc atgccggaaa gctcgccgat gatggtgtcc gcagaaggac
    11641 gtgagacgcg ggcacgttcg ccgttgttta tttgcaccag acctttgcgt tttaacgctg
    11701 ccagcgcttc gcgcaccgaa ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag
    11761 acggtaattg ttcaccttca ccaaattcac gacggcggat catctgttcc agctcttctt
    11821 ccaccatttc ggagagtttt ttacgcgcca gcgggcggct gcgcaagttg cgcccgatag
    11881 tggtagtaga atcttcagct tgcggatcaa atgcgttcat aggactcatt caggaaaatg
    11941 cgttagcgga taataacgac gctatcgtag cacagtatca aagtcaggcg aaaagatgac
    12001 agatagcaaa acaggccaca tgggcctgtt ttcattttac ttcacaacgc gtaatgccgg
    12061 tcgaccaccg cgtggtggct gcggaggttc atcgtcagga tgagtttcat catcgtgatc
    12121 tggcttgtcg ccatcgataa ccgacataac ggtttcgttg tctgccgatg cctcttcatc
    12181 attcatgatg ctggcatctt catcgtaggc agcttcaggc tcaaacatcg tgcctgcgcc
    12241 attttcacgg gcgtagatag ctagcacagc agccagcggc acagaaacct gacgcggaat
    12301 gccaccaaag cgcgcgttaa agcgcacctc atcattcgcc agttccagat tgccgacagc
    12361 acgcggcgca atgttgagta cgatttgccc gtcacgcgca tattccatag gaacctgcac
    12421 gccagggagc gtcacatcca ccaccaggtg cggcgtgagc tggttatcca gcaaccactc
    12481 atagaatgca cgcagcagat agggacgacg tggtgttagc tgtgacaaat ccatacagat
    12541 taactccggc ccagacgcat ttcacgttct gcttcagtta aagaagcaag gaaagagtca
    12601 cgttcaaaga cgcgggtcat atagcctttc agctctttcg cacccgggcc gctgaactcg
    12661 atgcccagtt gcggcagacg ccacagcaga ggagcaaggt agcaatcgac caggctgaac
    12721 tcatcgctca ggaagtacgg cttctgaccg aagaccggcg caatcgccag cagttcttcg
    12781 cgcagttgct tacgtgcggc atcggcttca gaagctgaac cgttgatgat ggtgttcatc
    12841 agcgtgtacc agtctttttc gatgcgatgc atgtacagac ggctttcacc gcgagctacc
    12901 gggtaaacag gcatcagtgg cggatgcggg aaacgctcat ccagatattc cataatgatg
    12961 cgagattccc acagggtcag ctcacgatcc accagggtcg gaacgctctg attcgggttg
    13021 aggtcaatca gatcctgagg cggattgtcc ttttccacgt gttcgatctc gaaacttaca
    13081 cctttctcag ccagcacaat gcggacctga tggctataga tgtcagtagg accggaaaac
    13141 agcgtcatta ccgaacgttt gttggcagcg acagccatga aaacctccag gtatagtcag
    13201 aatttttact gctaccagcc accaggtggc cagtcagaag ttgtgttacc cagtaaggaa
    13261 cgactctctt tgttcgaaaa tcaaacaaaa aatgagcaat acccgacatt tgggcagaaa
    13321 attggatgat agtttaccag attttgtgac ctttgtggtg agtcgatgcc ggaaatgggg
    13381 aaaaagagat gcgctttagt ctgaaatagt tgacttagtc ccttattggc gatgtggttt
    13441 ttgttttgcc tgttgtcaga cggcagcaaa aagcaacttt ccatttttta cgctgatgca
    13501 gattttagcc ataaaaaaac ccgccgaagc gggttttttc gcaaattgtt ttctgccgga
    13561 gcagaagcca attaacgttt ggagaactgc ggacgacgac gtgctttacg cagaccgact
    13621 ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg cagttcagaa
    13681 cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat cgcaccagcc
    13741 tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc aaccatgtcg
    13801 accagttcca gcggctgacg aactaccatg cgagcagttt cacgaccgaa gtactgttcc
    13861 agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac gcgagctgcg
    13921 gaacttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta taatcccgat
    13981 tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt gctcgttacc cgcgtaaact
    14041 ttcagtttac ggaacatagc acgacccagc gggccttttg gcaacatgcc tttaaccgcg
    14101 atttcaatca cacgctcagg acggcgagca atcatctctt caaaggtcgc ttgtttgata
    14161 ccaccgatgt ggccggtgtg gtgatagtac actttgtcag tacgcttgtt gccggttaca
    14221 gcaaccttgt cagcgttcag aacgatgatg taatcaccgg tatctacgtg cggagtgtat
    14281 tccgctttgt gcttaccgcg caggcgacga gccagttcag tagccagacg gcccagagtt
    14341 ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg tttctggttt agctgtaaaa
    14401 gttttcatta aaagcttacc caataaatag ttacacgttg gtgaacaccc aaacgtcttc
    14461 aattgttgag gttcacacga caaagtccgg caaacctacc ccttcgaata gcctatgcca
    14521 gcacacaaaa agtttcggga aaaaaaactt tcttgtaacg tggggtcgca ggattataga
    14581 gaagtcgggg tcaaagatcg accccttttt gtgatttgtg gcaggtttta ccccgccaaa
    14641 tgctcgcgct tcaaatactc ttcgctttgc atctcttgca gacgtgacag gcaacgctgg
    14701 aactcaaact tcagccgctc gccctgataa atttcataca gcggcacttc tgcactcacc
    14761 actaatttga catggcgctc gtaaaactca tccaccagcg caataaagcg tcgcgcttcg
    14821 ctctccatca accgcgtcat aactggtaca tcaaacaaca tgaccgtgtg aaagaagcgt
    14881 gagagcgcaa tatagtcatg ctgactgcgg gcgtcgacgc acagcgtagt aaaagaaacc
    14941 gccagcgtct ggttctcgac gcccattgtc gccaatggcc gatggttgat ttctaacgtc
    15001 ggtgaatttt ctcgtttcgc ccccgccagc gccaaccata gtttatccat ttgcgcccgg
    15061 gtttcatcgt tgaggggcga aagccacaga tgcgcctgag tgagtgtacg cagtcgataa
    15121 tcaacaccag cgtccacgtt cattacatca caatgctgtt taatagcatc gattgcaggc
    15181 aaaaaacgcg cacgttgcag gccatttcga taaagttcgt ccggcggaat atttgacgtc
    15241 gctaccagcg ttattccgcg agcgaacagg gctttcatca ggccgccaag tagcatggca
    15301 tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc tgctttaaag
    15361 cgatcggcaa taatttccag cggatcggtc tgcccctgca aagcagttag ctcttcatgt
    15421 acccgcagca taaaacggtg aaagtgcaga cgctgtttcc gctctcccgg caggctttga
    15481 tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat atataagcca
    15541 cgcactggcg tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac ccgcgccatt
    15601 agcccactcg tcctgggggc tggaggcgtg ctattgatga gttcctgata aataatttcc
    15661 aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc attgagcgcc
    15721 ttcaggtatt gcgatgttgg ggtaatgctt tgcatgatct tattgttatt ccttgaataa
    15781 tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac gcgatatgca
    15841 gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc gcaggcatgg
    15901 agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat gacctgggaa
    15961 tatgcgctaa ttgggttagt cgtcggtatc attattggtg ctgtggccat gcgttttggt
    16021 aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa taaagctgaa
    16081 ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga attactggat
    16141 accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc cagtagcctg
    16201 ctgccggaac tgtctgcgga agcaaacccg ttccgtaatc gtctggcaga gtctgaagcc
    16261 agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggcgc atccggcctg
    16321 ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt gcgccctcct
    16381 cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg tctgaaaatt
    16441 caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag agcaggaata
    16501 atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt aactctctcg
    16561 gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc ccccctcccc
    16621 agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg ggtggaagga
    16681 acgaccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg tgatgattta
    16741 ccggatcaac ctgcacaacc cttcgaaggt ttaggctccg gtgtcatcat caacgccaat
    16801 aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat cagtattcag
    16861 ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca gagcgatatc
    16921 gccctgttgc aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc cgactccgat
    16981 aaattgcgcg tcggtgattt tgccgtggcg gtcggtaacc catttggcct tgggcaaacc
    17041 gctacctccg ggattatatc cgcattaggc cgcagcggtc tgaatctcga aggtctggaa
    17101 aactttattc aaactgatgc ctccattaac cgcggcaatt ccggtggtgc gctgttaaac
    17161 cttaacggtg agttaattgg catcaacact gcaatccttg cgcctggcgg cggtagcgtc
    17221 gggattggat ttgccattcc cagtaatatg gcgcgaacac tggcgcagca gcttatcgac
    17281 tttggtgaaa tcaaacgcgg tctgttaggc atcaaaggca ccgagatgag tgccgatatc
    17341 gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt gttgccaggt
    17401 tctggctcgg cgaaagcggg cgtcaaagcg ggcgatatta ttaccagcct caacggcaaa
    17461 ccgctgaata gctttgctga gttgcgctct cgtatcgcga ccaccgagcc gggcacaaaa
    17521 gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac gctcgatacc
    17581 agcacctctt cgtcggccag cgctgaaatg atcacgccag cgctggaagg tgcaacgttg
    17641 agcgatggtc agctaaaaga tggtggcaaa ggtattaaga tcgatgaggt tgtcaaagga
    17701 agcccagctg ctcaggctgg cttgcaaaaa gacgatgtga tcattggcgt caaccgcgat
    17761 cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc cataatcgcc
    17821 ttgcaaatca tacgcggcaa tgaaagcctc tacctactaa tgcgttaatg tcgtaaaccg
    17881 ggcatcaggc tgacgtgtga tatccggtta actcgtggta tgctgctgcc gttccctttt
    17941 ttaacgatgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt ggattaattg
    18001 tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg ctttccactc
    18061 cgcaatttga cagtaccgat gagacgcctg ccagctataa tctggcggtt cgccgcgccg
    18121 cgccagcggt ggttaacgtt tataaccgcg gtttgaacac caactctcac aaccagcttg
    18181 agatccgcac cctgggatct ggtgtaatca tggatcaacg cgggtatatc atcaccaata
    18241 aacacgtgat caacgacgcc gatcagatca tcgtcgcctt acaggatgga cgtgtttttg
    18301 aagcgttact ggttggctct gactctctaa ccgatctggc ggtgctgaaa attaacgcca
    18361 caggcggttt accaacgatt ccgatcaatc cacgccgcgt accgcacatt ggcgatgtgg
    18421 ttctggcgat cggtaacccg tacaacctcg ggcagaccat tacccagggg attatcagtg
    18481 ccacgggtcg aatcggtctg aacccgaccg ggcggcaaaa cttcctgcaa accgatgctt
    18541 ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggcgaa ctgatgggca
    18601 ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt atcggctttg
    18661 cgattccttt ccagttagcg accaaaatta tggataaact gatccgtgat ggccgtgtga
    18721 tccgtggcta cattggcatt ggtggacgtg agatcgcccc attacatgcg cagagtggcg
    18781 gcatggatca actgcaaggg atcgtggtta acgaagtgtc gcctgatggc ccagcggcaa
    18841 atgcaggcat ccaggtcaac gatctgatta tctctgtgaa taacaaaccc gctatttccg
    18901 ccctggagac aatggatcag gtcgcggaaa ttcgccctgg ttcggtgatc ccggttgtcg
    18961 tgatgcgtga cgataaacag ttaacattgc aggtcaccat tcaggaatat ccggcgacca
    19021 actaaggtca gcgtaaaaaa acaaaaaccg gagtctgtgc tccggttttt tattatccgc
    19081 taatcaatta cttattaacg aactcttcgc ccagggcgat atctttcttc agcgtatcca
    19141 gcataccttc cagcgcgttc tgttcaaatg cgctcagagt accgatagat ttacgctctt
    19201 ccacgccgtt tttacccagc agcagcggtt gagagaagaa acgagcgtac tgaccgtcgc
    19261 cttcaacgta ggcacattcg acaacgcctt gttcgccctg cagtgcgcga accagagaca
    19321 gaccaaaacg tgcagctgcc tggcccatag acagggttgc agacccgcca ccggctttcg
    19381 cttcaaccac ctcagtaccc gcgttctgga tacgtttggt cagatcagcc acttcctgct
    19441 cggtaaaact aacgccagga acctgtgaca gcagcggcag aatggtaaca ccagagtgac
    19501 caccaataac cggcacttca acttcgcctg gctgtttgcc tttcagttcc gcaacaaagg
    19561 tgttggaacg aatgatatcc agcgtggtaa cgccgaacag tttgtttttg tcataaacac
    19621 cggctttttt cagcacttca gcagcaatcg caactgtagt gttaaccggg ttagtgataa
    19681 taccaatgca cgctttcggg caggttttcg caacttgctg taccaggttt ttcacgatgc
    19741 cggcgttaac gttaaacagg tcggaacgat ccatacccgg tttacgcgct acgcctgcag
    19801 agataagaac gacatctgca ccttccagcg ccggagtcgc atcttcacct gaaaaacctt
    19861 tgattttcac agcagtaggg atatggctca gatcgacagc cacaccggga gtcactggag
    19921 cgatatcata cagagagagt tctgaacctg aaggcagttg ggtttttaac agtagtgcaa
    19981 gcgcctggcc aataccgcca gcagcgccga ggactgcgac tttcatccta aactccttat
    20041 tatattgata aactaagata tgttgctccg ctgccgcgac cttaatccac aaaattgccg
    20101 tttacaatga ccacatctca agaatgtgta gtcacgcaag tttagcgttt atgcatttaa
    20161 ttgccgtaat caggaaccta acttacgtaa ttaacagtca cttatgcatt agcgcaacat
    20221 tccaacaggt ggtgacaata taccctaccg ctcagccaaa acaatatcaa tttgataaca
    20281 attaatttac ttttaagcag aatttgcagg ccgtgacgca ggcatgtttc tcaataacga
    20341 aatttgataa aatcccgctc tttcataaca ttatttcagc cttcttcagg gctgactgtt
    20401 tgcataaaaa ttcatctgta tgcacaataa tgttgtatca accaccatat cgggtgactt
    20461 atgcgaagct cggctaagca agaagaacta gttaaagcat ttaaagcatt acttaaagaa
    20521 gagaaattta gctcccaggg cgaaatcgtc gccgcgttgc aggagcaagg ctttgacaat
    20581 attaatcagt ctaaagtctc gcggatgttg accaagtttg gtgctgtacg tacacgcaat
    20641 gccaaaatgg aaatggttta ctgcctgcca gctgaactgg gtgtaccaac cacctccagt
    20701 ccattgaaga atctggtact ggatatcgac tacaacgatg cagttgtcgt gattcatacc
    20761 agccctggtg cggcgcagtt aattgctcgc ctgctggact cactgggcaa agcagaaggt
    20821 attctgggca ccatcgctgg cgatgacacc atctttacta cccctgctaa cggtttcacc
    20881 gtcaaagagc tgtacgaagc gattttagag ctgttcgacc aggagcttta atctctgccc
    20941 cgtcgcttct ggcggcgggg aaaatgttgc ttttgcctat caaccccctg ctttcccctg
    21001 cgattcattt aacgaatagt gcgttttact gcgacatatc attcacacaa tgaatacata
    21061 tagtaaaaa
//
LOCUS       DAZFRG010000065        20951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFRG010000065 DAZFRG010000000
VERSION     DAZFRG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.065"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(64..2511)
                     /gene="glgP"
                     /locus_tag="ACHKUC_001892"
     CDS             complement(64..2511)
                     /gene="glgP"
                     /locus_tag="ACHKUC_001892"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO6618156.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKSLFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2530..3963)
                     /gene="glgA"
                     /locus_tag="ACHKUC_001893"
     CDS             complement(2530..3963)
                     /gene="glgA"
                     /locus_tag="ACHKUC_001893"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO6618157.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3963..5258)
                     /gene="glgC"
                     /locus_tag="ACHKUC_001894"
     CDS             complement(3963..5258)
                     /gene="glgC"
                     /locus_tag="ACHKUC_001894"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO6618158.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5276..7249)
                     /gene="glgX"
                     /locus_tag="ACHKUC_001895"
     CDS             complement(5276..7249)
                     /gene="glgX"
                     /locus_tag="ACHKUC_001895"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO6618159.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIYG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQRGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7246..9432)
                     /gene="glgB"
                     /locus_tag="ACHKUC_001896"
     CDS             complement(7246..9432)
                     /gene="glgB"
                     /locus_tag="ACHKUC_001896"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO6618160.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9705..10808)
                     /gene="asd"
                     /locus_tag="ACHKUC_001897"
     CDS             complement(9705..10808)
                     /gene="asd"
                     /locus_tag="ACHKUC_001897"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6618161.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11000..11593
                     /gene="yhgN"
                     /locus_tag="ACHKUC_001898"
     CDS             11000..11593
                     /gene="yhgN"
                     /locus_tag="ACHKUC_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO6618162.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11639..12934)
                     /locus_tag="ACHKUC_001899"
     CDS             complement(11639..12934)
                     /locus_tag="ACHKUC_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO6618163.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(12841..14937)
                     /locus_tag="ACHKUC_001900"
     CDS             complement(12841..14937)
                     /locus_tag="ACHKUC_001900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO6618164.1"
                     /translation="MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(15077..16417)
                     /gene="gntU"
                     /locus_tag="ACHKUC_001901"
     CDS             complement(15077..16417)
                     /gene="gntU"
                     /locus_tag="ACHKUC_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6618165.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(16421..16948)
                     /gene="gntK"
                     /locus_tag="ACHKUC_001902"
     CDS             complement(16421..16948)
                     /gene="gntK"
                     /locus_tag="ACHKUC_001902"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6618166.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLRAGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(17087..18082)
                     /gene="gntR"
                     /locus_tag="ACHKUC_001903"
     CDS             complement(17087..18082)
                     /gene="gntR"
                     /locus_tag="ACHKUC_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO6618167.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(18175..18870)
                     /gene="yhhW"
                     /locus_tag="ACHKUC_001904"
     CDS             complement(18175..18870)
                     /gene="yhhW"
                     /locus_tag="ACHKUC_001904"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO6618168.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(18993..20030)
                     /locus_tag="ACHKUC_001905"
     CDS             complement(18993..20030)
                     /locus_tag="ACHKUC_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6618169.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVTLAR"
     gene            20368..20856
                     /gene="yhhY"
                     /locus_tag="ACHKUC_001906"
     CDS             20368..20856
                     /gene="yhhY"
                     /locus_tag="ACHKUC_001906"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6618170.1"
                     /translation="MSEIVIRHAETRDHEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         5167 a         5697 c         5151 g         4936 t
ORIGIN      
        1 aataccgtga gttccactct gtattacttc agcggaacac acggtattta gccgtatttg
       61 aacttacaat ctcaccggat caatatgcca gatatgatcg gcgtactctt tgatcgtacg
      121 gtcagaagag aagtagccca tattggcaat gttcagcatc gcttttgcgg tccactcttc
      181 ctgacgctcg tagagttcgt cgactttatc ctgacaatcg acatagctgc gataatccgc
      241 cagtacctgg tagtgatcgc cgaagttgat cagcgaatcg accagatcgc gatagcgacc
      301 cggatcttcc ggactgaata caccgctgcc gatttgcgtc agcacctgat gcagctcctc
      361 atctttctcg tagtattcac gcggtttgta gccctgacga cgcagttctt ccacttcttc
      421 cgctgtgtta ccaaagataa agatattgtc agcaccgaca tgatccagca tctcgacatt
      481 cgcaccgtcc agcgtaccga tagtcagcgc gccgttaagc gcaaacttca tgttactggt
      541 gccggaagct tccgtccccg ccagcgaaat ctgttcagat agatcggccg ccggaatgat
      601 caactgcgcc aggctaacac tgtagttcgg gatgaacacg actttcagct tgtcgccaat
      661 ctgcggatcg ttgttgatca ctttcgctac gtcattgatc aaatgaataa tgtgcttcgc
      721 catgtaatag gccgaagccg ccttaccgcc aaaaatattc acgcgcggta cccacttcgc
      781 atccgggtcg gccttgatgc ggttatagcg ggtgatcacg tgcaacacat tcatcaattg
      841 acgtttgtat tcgtgaatac gtttgatttg cacatcgaac agcgatttcg gatttaccac
      901 cacattcagc tgctgggcga tatactccgc cagacgcttt ttgttctcca gcttcgcctg
      961 atgcaccgcg tgattaacca ttgggaaatc acagtgttgt tgcagatcat taagcaggct
     1021 aaggtcggtg cgccagttgc ggcccaggtg ttcgtccaac acggctgaaa gcgatgggtt
     1081 cgctaccgcc agccagcgac gcggcgtcac accgttggtg acgttggtga aacgacccgg
     1141 gaagattttc gcaaagtcgg caaatagcga ttgcaccatc aggttagagt gcagctccga
     1201 tacaccgtta actttgtggc tcaccacaac cgccagccag gccatacgca cacgacgacc
     1261 gtttgattca tcaatgatcg acgcccgtcc cagcagatcg gtatcgttcg gatactgttc
     1321 ctgcaaggtt ttcaggaaat agtcgttgat ttcaaagatg atctgcaggt gacgcggcag
     1381 aattttaccc agcatatcga ctggccaggt ttccagcgcc tcgctcatta gcgtgtggtt
     1441 ggtgtaggag aagacctgac aacacacttc aaacgcgtcg tcccagctaa attggtgctc
     1501 atcgatcagc agacgcatca tctcaggaat cgacagcacc ggatgagtat cgttgaggtg
     1561 aatcgcaatt ttatccgcca ggttatcgta ggttttatgc aactgataat ggcggcttaa
     1621 aatgtcctga atggtcgagg aaaccaggaa gtattcctga cgcagtcgta gctcacgccc
     1681 ggagtaggtg gagtcgtccg gatacagtac gcgagatacg ttctcggagt ggtttttatc
     1741 ttccactgcc gcgaagtagt cgccctggtt gaatttaccg aggttaattt cgctactggc
     1801 ttgggcactc cacaaacgca acgtgttggt cgcgtcggtg tcgtaacctg ggattatctg
     1861 atcgtaagct actcccagaa tctcttcggt ttcaatccag cgcgtttttt taccttcctg
     1921 ctgaatgcga ccgccaaaac ggactttata gcgcgtgttg tggcgtttga attcccacgg
     1981 gttaccgtat tccagccagt agtcaggcga ctctttctgg ctaccgttaa cgatgttctg
     2041 cttgaacata ccgtagtcat agcggatgcc gtaaccgcgc cccggcaacc ctaacgtcgc
     2101 cagagaatcg aggaagcaag ccgccagacg gcccaggcca ccgttaccga ggcccgggtc
     2161 attttcttca tcaatcagct cttcgagatt taatcccatc gcttccagtg cgccctgtac
     2221 atcttcgtaa attcctagcg acaacatggc gttggaaagc gtacgaccaa tcaaaaactc
     2281 catcgacagg tagtaaacct gacgagtttc ttgcgacaac tgggcacggt ttgagcgtaa
     2341 ccagcgctcc acgagacgat cgcgcacagc aaataacgtt gcgttcagcc attcatgttt
     2401 attggcgacg accggatcct tgccaatcgt aaacatcagc ttgtaagcga tagagtgctt
     2461 aagagcttct acgctaagcg tgggcgatga atatgtaaac ggagcattca tataggcgtt
     2521 tcctgaaaac tatttcaagc gatagtaaag ctcacggtac gacttcgccg cgacctgcca
     2581 gctaaaatcc attgccatag cctgacgttg cacaaaccgc cacagtgaag gacgggacca
     2641 cagtacaaaa gcacgtcgaa tagcccgtaa cagcgaccag gcattactat cttcaaagac
     2701 aaacccactg gcgacgccat ctgcaaggtt ctcaagagaa cagtcagaaa ccgtatcagc
     2761 aagcccaccg gtgcgccgca ctaacggcag cgtaccgtac ttcaatccat aaagttgcgt
     2821 taagccgcac ggttcaaaac ggctgggcac cagaatgacg tccgcgccgc ccataatgcg
     2881 atgcgaaaat gcttcgtgat agccaatctg aacgcccacc tggccggggt attccgctgc
     2941 cgccgcaagg aaaccttcct gcagcaccgg atcgcccgcg ccgagtagcg ccagctgccc
     3001 gccctgctcc agaagaccag gtaaggcttc cagcaccagg tcgagacctt tctggctggt
     3061 cagacggctc accactgcaa aaagcggcac tttatcgtca accttaagcc ccattgcgat
     3121 ttgtaactgg cgcttatttt ccgctttatc ttccaacgta tcgcgggtgt aacgcgaggc
     3181 caacagtaag tccgtctctg gactccagat tttctcgtcc acgccgttca gtacgccgga
     3241 aagacgccct tcacggtgac gctgttgcaa cagaccttcc ataccgtagg caaactgcgg
     3301 ttcggtgatc tcgcgagcgt aggttgggct gactgccgta atgtgatcgg catagtacag
     3361 accggccttc aggaaagaga tttgtccgtt gaattccagc ccatgaatat taaagaatga
     3421 ccatggcaat tggatgtcat tcatgtgatg tgcataaaac atgccttgat aggccaggtt
     3481 gtgcacagta aacaccgact tcgccggacg cccgcgcgcc gccagatacg caggcgcaag
     3541 gcctgcatgc cagtcgtgcg catgcaccac atcaggacgc cagaatgggt caagcccgct
     3601 ggccatttct gccccaaccc accccagcag cgcaaaacgc aatacgttgt cggtataggc
     3661 aaataagttg gtatcgtgat acgggcttcc cggacgatca tagagatgcg gcgcgtcaat
     3721 caggtatatg ccaaccccgt tgtaatgacc gaacaacagc gtgatatgtc cggcgaaggt
     3781 atcacgacgg gatactacct gcgcatcggt cacgccacgg cgaatatcgg gaaatgcagg
     3841 caacagtacg cgagcgtcaa cgccgtctgc gatttgtgct gcgggtaatg ccccaataac
     3901 atcagccaga ccgccggttt taagcagcgg gaacatctct gaacatacat gtaaaacctg
     3961 cattatcgct cctgtttatg ccctaacttc cgtagcattt cgcgcgttac cagcacgatg
     4021 ccttcttctg aacgatagaa acgacgtgca tcttcctctg cgttttcacc aatcaccatg
     4081 ccttccggaa taacacaagc acgatcgatg acgcagcgac gcagacggca cgagcgacct
     4141 acccatactt ccggtaacaa tacggcggaa tcaatgttgc agaatgaatt cacgcgaacg
     4201 cgcgagaaca gaacggactg caccaccacc gaaccggaga tcacacaacc gccggatacc
     4261 agtgagttaa gggtcatccc gtggctaccg gagcgatcct gcacgaattt cgctggcggt
     4321 aatgattcat tgtaggtgcg aattggccaa ttgcgatcgt acatatccag ttccggcacc
     4381 acagaggcca gatcgaggtt cgctttccag taagcttcca gcgtacccac atcgcgccag
     4441 tacggctcgg catccgggtc ggattgtacg caagagagcg ggaacgggtg cgcataagcc
     4501 agaccggctt cggtgatctt gggaatcaaa tctttgccaa agtcgtggct ggagttctca
     4561 tcgcgatcgt cttcttccag cagttcatac agatagtcgg cgtcaaagac gtagataccc
     4621 atactcgcca gagatttgct cggatcgttc ggcattgacg gcgggttagc tggtttttca
     4681 acgaattcga taattttatc gttctcatca accgccataa cgccaaacgc ggaggcttct
     4741 tcaatcggta ctggcataca agcaacggtg caacgtgcgc ctttttcgac gtggtcgata
     4801 agcatacgcg agtagtcttg cttgtagata tggtcgcccg ccaggatcac cacgtattcc
     4861 gctttataac ggcggataat gtcgaggttt tgggtgaccg catctgcggt gccgcgatac
     4921 cagttttccc ctttcattct ctgctgtgct ggcagcagat cgacaaactc gttcatttct
     4981 tcattgaaga atgaccagcc gcgctggatg tgctgcacca gagtgtggga ctggtactgg
     5041 gtgatcacgc ccatacgacg gatcccggag ttgatgcagt tagacagcgc aaagtcgata
     5101 atgcggaact taccgccgaa gtgtacggcc ggttttgctc gcttattggt taaatccttc
     5161 aggcgggtac cacgtcctcc cgccagtatc agggcaacag atttcaatgg cagctggcgc
     5221 gccaacatta agtgatcgtt cttctctaaa ctaaccatga ctaactcctt ttttatcatc
     5281 tctggaacac acacaatccg tgtgcaggtc cctgccagac agccgtaatc actgggttat
     5341 cctctccagc gaatggggga atggcgtgcc actccccagc aggtaaaaca atctctgtta
     5401 cctcaagcgt ggcgttaatt gcgatcaaaa agcgatccga gagcagaatt tgcagctgtt
     5461 tcggcccgtt ttgccactca tccgtgctta aaggttgagc atatcgattt agccaacgga
     5521 cattgccgtc gccttcttcc caccagcgat tctccatcaa agcgggaatg cgcttgcgca
     5581 gatggattaa cgcggcggta aatgcggtta aaccactgct tgcctgcgac cagtccaacc
     5641 aggttaattg gttatcctga cagtaggcat tgttattgcc acgctggctg tgaccatgtt
     5701 cgtcaccggc cagtaacatc ggcgtaccct gggagagcaa caacgttgtt aacagggcgt
     5761 gaatgctgtc gcgccgccgt tcaaccagat caagagtacc gcctaaccct tctttaccat
     5821 gattgttact gtaattgttg ttggtcccgt cgcgattttc ttctccgttt gcttcattgt
     5881 gtttatggtt gaagcaaacg cagtcgcgaa gcgtaaaacc gtcatgcgcg gtgacgagat
     5941 taatcgcggc actcggcaga cgaccattac gtttaaaaac atcgctggag gcagcaaaac
     6001 gcccggcaaa cgctcccaga ggtaaatcat aatgcagcca gaaacgacgg gctgcatcgc
     6061 ggaaatgatc gttccactcg gcaaacagcg gcgggaaatt tcccacctga taaccaccag
     6121 gagcgatatc ccacggttca gcaattaact tcacctgcga gagcaccggg cagttctgga
     6181 tagcggtaaa caacggcgca tcctgacgaa actctggcgt acggcccatg actgccgcca
     6241 gatcaaagcg gaaaccatcg acgtggcagg tttctaccca ataacgcagg caggcgctgg
     6301 cataatctac caccgccgga tgactcaaat tgagcgtgtt accacaaccg gtccagttgt
     6361 gataatcgcc gtcttctctt atccaataat agctacggtt gtcgatccca cgcagcgaga
     6421 ataacgggcc gtcgaggtcc agttccgcac tatggttgag cacgatatca agaatgactt
     6481 cgatacccgc tttatgcagt gctttgattg catcgcgaaa ctcatccagc gccgtttctg
     6541 gcgagcaggc atacgccgga tgcagcgcaa acatcgccac cgggttgtac ccccagtagt
     6601 tactaagccc catgcgttgc agacgtggtt cgctggcaaa ttgtgccacc ggcagcagtt
     6661 ccagcgcggt aatgcccaac tgtttcaaat agttgatcat caccggatgc cccagggctt
     6721 tataagtgcc acggatctcg accggaatct ccgggtgcag gtacgttaat cctttgacat
     6781 gggcttcata aatgatggtg ctgccccacg gcgtgcgcgg cggagcatca tcttcccagt
     6841 catagtgatc aaccaccact acgcatttcg gcgcaatggc ggcgttgtcg cgatagtcag
     6901 gttcattatg accggcgtgc agcagcgggt tatctttaaa ctccccgtaa atttgccgcg
     6961 cgcaaggatc aatcaacaac ttcgccgggt taaagcgatg cccctcggca ggttgccagg
     7021 ggccatgaac gcgataacca taacgcaaac ccgggcgcgc atccggcaga taaccgtgcc
     7081 aaatgtcgcc actgtgccct ggcaagtcat agcgatgttc ctggccattg gcatcaaaga
     7141 cacacagctc tacccgctcg gcatgagcgg agaaaagtgt gaagttgacg ccctgaccgt
     7201 cgtaatgcgc gccgagggga gtgggtttgc caatggcgag ttgtgtcatt ctgcctcccg
     7261 aaccagccag atagtggcca gaggtggtag cgtcaggctc agtgaatgct gacgaccgtg
     7321 gctggcaatc tcatcgctgt gtactgcgcc gccattgcct gcattactgc cgtgatagtg
     7381 catggaatcg gtattgagga tttcacgcca tttacccggc tggttaatac cgaagcgata
     7441 atcatgacgc ggcaccggtg taaagttact ggcaacgatg atttcgttac cctctttatc
     7501 gcgacgcaca aagatcagca ccgagcgttc tttgtcatcc accaccagcc attcaaagcc
     7561 gtacgggtca aaatccagtt catgcattgc tttatggtgg cggtaggtgt ggttcagatc
     7621 gcgcaccaga cgctggacac cgtggtgcca gttatcgccg ccttccaaca gatgccagtc
     7681 gaggctggcg tcatggttcc actcgcggcc ctgggcaaat tcgttcccca tgaacagtag
     7741 tttcttgccc gggaatgccc acatccagcc gtagtaggcg cgcaggttag cgaatttctg
     7801 ccatgcgtcg cccggcatcc ggtcgagaat cgatttttta ccgtggacca cttcatcatg
     7861 cgacaacggc aggacgaagt tttcagtgta gttgtagagc atcccgaagg tcagtttatc
     7921 gtgatgatac tgacgataaa tcgggtcgag cttcatgtag tccagggtgt catgcatcca
     7981 gccgaggttc cacttgtacc agaagcccag accgcccata tcctgcggac gagaaacgcc
     8041 agggaaatcg gtagactcct ccgccattgt cactgcaccg gaaacctgct caccaagaat
     8101 acggttggta ttacgcaaga attcaatcgc ttcaagattc tcgcgaccgc caaattcgtt
     8161 cgggatccac tccccctctt tacggctgta gtcgcgataa atcattgacg ccaccgcatc
     8221 gacgcgcagc gcatcaatac caaaacgttc aatccagtaa agtgcgttac cgacaaggaa
     8281 gttactgact tcacggcgac catagttgta gatcagcgtg ttccagtcct gatgatagcc
     8341 ttcgcgcgga tcgctgtgtt catacaagtt cgtgccatca aattcggcaa gcgcaaagtc
     8401 atcggtcggg aagtggcctg gtacccagtc gagaatcacg ttcagaccag ctgcgtgtgc
     8461 ggcatcaatg aaataacgga agtcgtcgcg agtaccaaaa cggcgggtcg gtgcatacag
     8521 gccggttggc tgataacccc aactgccatc gaagggatgc tcgttaatgg gcagtagctc
     8581 gagatgggta aagcccatcc atttagcata aggcaccagt tgatcggcca gctcgcggta
     8641 gctcaaccag aaattgttgt cggtgtggcg acgccaggag ccaaggtgaa cttcataaat
     8701 agagattggc gcatcaaact gattcgcttt tttgcgctct tcagtctgca caaccttttc
     8761 cggcagcccg caaataagag acgcggtttc cgggcgcatt tgcgcttcga aggcataagg
     8821 gtcggacttc agacgcaagt tgccattggc atcaatcatc tcgtatttat agagctgacc
     8881 gttatgcgcc ccagggataa acagttccca gattccgctc tctttacgca gtcgcatcgg
     8941 atgacggcga ccgtcccagt agttgaattg cccaaccacc gagacccgac gggcgtttgg
     9001 agcccagaca gaaaaacgcg tacctgtgac gccatccata gtatctgcat gcgcgcctaa
     9061 ggtttcatac ggacgtaggt gagtaccttc agataatagc caggcatcca tttcctggat
     9121 tagcgggcca aaacggtaag gatcatcaat caggttttgc tgaccatgcc agacaacagc
     9181 caactgatag cggaaaaaat tcttacgtcg cggaatgaca ccgctaaaga atccccgtga
     9241 gtcgagacac tccagttttg cgagtttgcg cccggttttc ggttcaatca cccacacatc
     9301 ggtagcgtcg ggtaaaaggg cacggacttc cagtcccgcg gtggttttat gcatccccag
     9361 tacggaaaaa ggatccgcaa aatggcctgc aattagcgcg ttaatcacgt ctctatcgat
     9421 acgatcggac atgcttgtct tcctgtttta ttgtgtcacc ccatccaact gaatttttga
     9481 cttctggttg tgacattttt ttgacctgaa cggcgcagca ctctgtgcat cctctctgcg
     9541 tcgtcctcac ttcaggtaag gctgtgaata ctcgtgtatt ccgccaccct taaagaatag
     9601 ccaatgctct atttaactcc cggtaaatca tgaaacatct gcgcttactc ctgtattacg
     9661 cactagcagg ggcggcatcg cgccccagat ttaatgaata aagattacgc cagttgacga
     9721 agcatccgac gcagcggctc cgcggccccc cacagcagct ggtcgcccac ggtaaaggct
     9781 gacaggaact ccggtcccat attcagctta cgcaggcggc ctaccggcgt ggtcagcgtg
     9841 ccggtaacgg cagctggggt tagctcacgc atagtgattt cccgatcgtt cggaacaact
     9901 ttcgcccacg gattgtgcgc agccagcagt tcttccacgg tcggaataga cacatctttt
     9961 ttcaatttaa tagtgaatgc ctggctgtgg cagcgcaatg ccccgacacg cacacataaa
    10021 ccatctaccg gaattacgga agatgtgttg aggatcttgt tggtttccgc ctgccctttc
    10081 cactcttcgc ggctctgacc gttatcaagc tgtttgtcga tccacggaat caggctaccc
    10141 gccagcggca cgccaaagtt atctaccggc agctcaccgc tacgggttaa ggttgtgact
    10201 ttgcgttcga tatcgagaat agcagaggac gggttcgcga gttcatctgc cacatggcca
    10261 tacagatggc ccatttgggt taataactca cgcatatgtc gcgcaccacc gccggaagcg
    10321 gcctggtagg ttgcaacgga cacccaatca acaagatcat tggcgaataa accacccagc
    10381 gacatcaaca tcaggcttac ggtacagtta ccgccaacaa aagtcctgat gccattattt
    10441 aatccgtcgg taatgacgtc ctgattgacg gggtcaagaa tgatgatggc gtcatctttc
    10501 atgcgcagag atgatgctgc gtcaatccag taaccttgcc atccgctttc acgaagcttt
    10561 ggatagattt cgttggtata atcgccgccc tgacaggtca caatgatatc gagggccttt
    10621 agcgcctcca gatcaaaggc atcctgaagt gtgccagtgg ttccgccaaa agacggcgca
    10681 gcctgaccaa gctgagaagt agaaaagaag acagggcgaa tggcgtcgaa gtcgcgctct
    10741 tcaaccatgc gttgcatgag aacggagccg accataccgc gccagccgat aaaaccaaca
    10801 tttttcataa gcgttttttc ctgcaaagat gtgtgctgta taaatgtgcc ggtctcctcc
    10861 tggcacatct ttcaccatac aaaaagcagc caaagtcgca agtgaaatta atcaatgata
    10921 gcgaagccat cagtaatgcg acttatcctg ctttgttagc acgcagaaag tccgcggcaa
    10981 ttatcaggga atttgagtta tgaatgaaat catttctgca acagttttat tgatcctgat
    11041 tatggatccg ctcggaaacc tacctatttt catgtccgta ctgaaacata ctgaaccgaa
    11101 aagacggcgg gcaatcatgg tgcgagagtt gcttattgct ctccttgtga tgctggtgtt
    11161 cctgtttgcg ggcgagaaaa ttctggcatt tcttagcctg cgggcagaaa ccgtctccat
    11221 ttctggcggc atcattctgt ttctgatcgc cattaagatg attttcccca gcgcttcagg
    11281 aaatagcagc gggcttccgg caggtgaaga gccatttatc gtgccgttgg caattccgct
    11341 ggtcgccggg ccgactattc tcgccacgct gatgttgttg tctcatcagt acccgaatca
    11401 gatgggacat ctggtgattg ctctgctgct ggcctggggc ggcacctttg tcatcctgct
    11461 acagtcttcg ctatttttac gtctgctggg cgagaaaggg gtgaacgcac ttgaacgcct
    11521 gatgggattg attctggtga tgatggcaac tcagatgttc ctcgacggca ttcgaatgtg
    11581 gatgaagggg taaggttcaa acaggtgttg cacacaacca gacgcggcac ctgttttatt
    11641 acctgaactg ataatgacct tcgcccaatc ggataaccct tccctcttct tctgcgcggt
    11701 tcagccacac actaatttgt tcgatggtta aaccagtaca agacgccagc ctttcaatac
    11761 ttatcgattc ctttgcaagg atcgccagtt ccgccacaaa gagttgataa aaatcgacag
    11821 gggtatgcgc gggcattact gcgtaatttg cgtcatcttc gtgcagcgaa aacagttctg
    11881 gttgagcgtt acggagtgct ctgtcataca tcagcccagg aacgtctggc agtctgatcg
    11941 tcacattctc agcctgttca gcactccatg acgccccatt ttctactaat cgtgaattgg
    12001 ctgattgcat atcctgattt ggtttaacct gcataggcag ccactgatgt tttaatgcct
    12061 ccatcgcccc ggttatcgtt ccaccggtca ttcccgcacg tacaaccatt gcgctttcag
    12121 caaggcaata aatatattta ttccgcgcca tcgcattgcc gacggtgaaa cgtacctctg
    12181 ggtaaaacgg cgaaatcaac accaggttgc ctgctataag cccttcacgc catttcacta
    12241 acgtactcgt ttttaacaag ctatcagcta atacgccaac ggcagttccc ccgacctcca
    12301 gagccgaggc cattgcacat tcatcaatac ctcgcgcacc accagagata acgcaaatcc
    12361 cctgttgggc cagtttcgcg gccagttgtt gggtatagcg caaatcgtca gtcggagcat
    12421 ctcgcgagcc aacaatcgcc ataccttccg cttgcagtaa tgctttattc ccgcagccaa
    12481 ataaaacggg aggtgcatcc gttcgcaatc ggtttttcaa gcgaacggga taatcggcat
    12541 ctccgcgggt taagatccat aaaccggcgc gttgccactt atctacagcc aacgccagac
    12601 tatgaccacg cgccagcaag ccaagaatac gctcctgaga aatacgcgga tcgctccagc
    12661 ctgtaagttt ctcttgcggt tgcggcacca gaagctcggc gggactgata cgttggtgct
    12721 tcagccataa tgcaaacttc ccccattcac tattactaag aggtttatac tcactcgccg
    12781 ccgcgcgaga aaaatcgctg gttagcagga gagttgcttg tgcattggct gaaagattca
    12841 tcaatttttt accgaggtag aagcaagggc aagaggataa accgtcgggc aacctgcctg
    12901 gcgtaacagt gctgtcagaa ctgtcagcgt ccatgcagaa tccacgatat catcaaccag
    12961 caacgccgga cctggcatca aaggaggggt aatcacaaac gccccgtcga gattttgaca
    13021 ctggtgaaaa cggttttgct gcattttctg cggtggattg tccacgactt tttcaatggc
    13081 atcaataaaa ggtaagccaa gtttcgccgc cagtcgccgg gcaaaatcag gaaccaggtc
    13141 gaggtgcctt aatgaaggaa cgcagcatac ccaggttggt tcaggatgcg gattccagcg
    13201 ttggcgaacc atttccgcac atgcctccac cagttcatca ctgaagcgcc ccgttttttt
    13261 ctcctgggct acctgctttc cccacccgga atctccccaa cgagaaagga ttcgccccgt
    13321 agatccttgt aaacccgcag gcaagttccc tttaaatccg tattgcgtaa aagccccaga
    13381 tgccacctgt ttattgagat ttaatggcag gtcagcgtgt ttaataaata aatttgccgc
    13441 atggagtaag ccgctatcaa tgtcgggcga caacagcgga tgctgaagac aactgctaca
    13501 tttaccacaa cgttcggcac ttggctcatc gagtgcacgg cgcagaaatt gcattttgca
    13561 ctccttggtc gtgatatagc tttcaacgtc tgccagttca ctttttctct gattcatcaa
    13621 atggttaatt cgttcatgat caggagaaaa actgacgata gttctgcgcc ataatttctc
    13681 ggtatacacc acaggcgatg gattttccgc taccaataat ttcagtgctt tttctatttg
    13741 cccataacga agattcgtcc gttgttcaat gcctcgtagg gtaagaccgt cattctcgct
    13801 aagtacgttg agtatttcat gaatttgcgc ctccgcagga aaggcgcttt cacggaagaa
    13861 ttgatgaata gcgcggtctt caccaccaca aagcaatatg ccaactgcgg aatctatagc
    13921 acgcccggca cgccccacct gttggtagta accgacaata gaccccggca tctgataatg
    13981 aatgacgaaa cctaaatcag gtttatcaaa ccccatacct aatgccgtcg tcgcgaccag
    14041 cactttgatt ttatttgcca gcagtgcctg ctccagatat tccctggcag tattagaatc
    14101 ttccgcgcct tcacaagtca cgccgctgta gtaagcaaat gcactgatac catttttcct
    14161 taaccactcg gcgacaagtt cagcatcgcg agtagtcagg gtataaacaa ttcccgattt
    14221 ggaaaactga gggataaccg ttgctaacca tgccagacgg gatgactgtt cacctaagac
    14281 taaggcatca agcgccaaac tttcgcgagc cagcgttcca cgctgaatca caatgtcacc
    14341 caattgctga cggatatcct caacgacacg gttattcgct gtcgcggttg tacccagaac
    14401 tggggtattc gcaggcagtt ggcgcaaaat atctaatata cgtcggtaat ccgggcgaaa
    14461 atcgtggccc caatcagaga tgcagtgcgc ctcgtcgacc acaagcaagc caatacgatc
    14521 ggctatagga tataaaaccg tttcgataaa gtcctggttt gccaaacgtt ccggggaaac
    14581 cagcaagcaa tcgacctcac cttgcagcaa cttatcgcta atacgctgcc attcttccct
    14641 gtttgtagaa ttcaacgttt cagccgtaat acctaaccgt tcagcggcgg caacctggtt
    14701 acgcatcagg gcaagcaaag gagagatgat aattgtcggg ccagcaccgc gatcgcggaa
    14761 gattttactg gcgatgaagt acacggcact tttcccccag ccagtgcgtt ggacaaccag
    14821 cagttttcgt cgttggttca ccaccgcgtc tatggcttcc cattgccctt ccctgaaagt
    14881 agcggtggta tttcctaaca tccgctgtaa aagcaattca gctccgtgct tttccatctt
    14941 tatctcctgc gatctttagg cagtgattag aacggattat tagcaggtaa atttgcgaga
    15001 gagatcaaaa agatgaagag aggagataag agaaatgaat tactgcgagc aagctcgcag
    15061 taatttgtcg cagtaattaa ctcaacagct ggaacgcaat catcccaacg atggcaccaa
    15121 cagtgccgag gatggtttcc atcatggtcc aggttttaag cgtttcggct tcggtcgcgc
    15181 cggtaaattt accgaacaac cagaaacccg cgtcgttaac gtggctgaca acaatcgaac
    15241 caccggcgat acaaatcgac agcgccgcca tttgcgcacc ggagtagttc agttgttcaa
    15301 tgaccggcat taccagtccc accgccgtta aacaggcaac agtggcagaa ccctgaatga
    15361 tgcgcactgc agctgccagc acgaagcagg tgatggcaat cggcaggccc atgccggtta
    15421 acgcttcgcc cagtgccgga ccgacaccag agtcaaccag cacctgtttg aacacgccgc
    15481 ccgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg caaatctcca
    15541 tcactttgtc ttttggcata ccctgacgca ttgccagacc gtaaatcgcc accaggcaag
    15601 caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa gcggtagagc
    15661 cttccggcac aaagcgcgcg gcaatggttt tcagccccac cagcaccagt ggcaacagga
    15721 tcagcgacag gctgaatccg aaggacggca ttttgccttc gccgagatgc ggttcgctga
    15781 tgtcgtcagg aatatgcagc tccacgtagc ggctgatgaa attaccccac agcggcccgg
    15841 caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag ccaaagtcgg
    15901 cgttcatctg cgatgccagc agcattggcg ctggtccagg caccaggaac gcagccgcgg
    15961 ccgccacccc tgcaaataat gggattacca gcttcaccag gttcgtaccg gtgtggcgcg
    16021 ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac aacggcagtg
    16081 cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg aaggatttga
    16141 gcattttgac ggcaatctga tcgactgcgc cggtttcatg taagatcttg ccaaacatgg
    16201 ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct ttttccatcg
    16261 tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccata gacaccacca
    16321 ttaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc agtaaaacag
    16381 accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct tttttaataa
    16441 cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc accagtacat
    16501 cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcactaac atttgggttt
    16561 taaagaaatg gcctttgcgc gctttcaggc ggctttcaat cacatcaaaa tcgcctttca
    16621 ggtagatgaa cgagagattc ggattacccg cgcgtagcaa gtcgcgatag tgttttttca
    16681 gtgcagaaca gacgatcagc gacactttat tggtgcgctg catagcaaac gcggcgtcgt
    16741 tcagcgcctg caaccacggt ttgcgatcgt cgtcattcag cggttcacca gacgccattt
    16801 tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca tgaagttgat
    16861 gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc aagacgtaaa
    16921 tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag aattaaaaat
    16981 gttacgggta actgttatcg gtaacattgt ccggccggac aatatcagaa gcaatatcca
    17041 tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa atagatccgc
    17101 ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg ccacgaatac
    17161 gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc agtacgctcg
    17221 ccaaccgtgg ttccatcacc tgaccaatgt catgaccatg gaaaccggca atcgccatat
    17281 cgtcaggaac tttcaacccc agacgctgac attcaaacgc cgcgccgacc gccaggtcat
    17341 cattagtaca gaacacgcca tccagctgcg gatattcccg cctcgcctgg cgaatcagtt
    17401 caataccgga agagtaagaa gaagattgct caaccatcac gctatatggc accaggcctg
    17461 catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg tcgagacgtg
    17521 cgccgagata ggcaatgtgg cgatgcccgc gagcaataat ggcggtggtc atctggcgtg
    17581 ccgcttcaaa gttatcaaaa ccgacggcaa tatcgaggca cggcgacttg ctgtccatca
    17641 gttccaccac tggaatcccc gccacttcaa tcatctttaa ggtgcgcggc gtgtgcgtac
    17701 gttcagtgag gatcaggccg tcgatattcc aggagagcat ggattcgagg cgttcttgct
    17761 ccatttccgg tttataaccg tagtgggcca gcatggtctg ataaccgtgc gcgtcggtga
    17821 cgctttcgat tccgcgtaat acttccgcaa aaacctggtt ggtgagagaa ggtaacagga
    17881 cgccaatcgc ccggctggtg gcgttagaga ggatatcggg tgcgcgattg ggaatataac
    17941 ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc tgctccgggt
    18001 tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc acatcctgaa
    18061 gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt ctcttagttt
    18121 aacacggacg catcagaacc ttcccgattc ctcaggaagg tttgtgagat aaatttagac
    18181 tggcggtaaa tcgaacagca ggatttcact gtcgctatca gcatggatgg aaatcgcctg
    18241 ctcatcccag attgccagac catcgctggt cgaggctttc acgccgttaa tggtgacatt
    18301 gcctttcacc acctggatcc agacgcggcg ttcagcggca atctgatgca ccgactgctc
    18361 atctttcacc agcgcccaac gatacagttc catatcctga tgcactttca atgaaccatc
    18421 acgcgcatcc ggcgaaagca ccagttgttt gccctgcacg gcatcgaagc gacgctgttc
    18481 ataacgcggc gtaatgccgt tttcttcagg catgatccag atctgataca gatgcagacg
    18541 ctcggtgctg cttgggttgt actctgagtg acgaataccc gtacccgcac tcataatctg
    18601 gaactcgccc gccggaacct gctctttatt gcccatgctg tcctgatgct caacagtacc
    18661 ttccagcacg taggtcaaaa tttccatatc tttatgcggg tgagtgccga agccctgccc
    18721 tgcttcaatc acgtcgtcgt taatcacgcg cagcgcagag aagcccataa aattcggatc
    18781 gtaatagttg gcaaaagaga aagtatgcca ggagtccagc cagccatgat ttgcatgacc
    18841 gcgttcattt gctttgcgta agtagatcat ttgtattcgc cccctgaatg atttcgatgg
    18901 gcttagtgtg gacccgatcc gcctgggatg atagagggtg aaaattgacc cctctgttca
    18961 aaaaaattga actattcgag gggccatcaa acttacctcg cgagagtcac tgcggaggga
    19021 gaagcttgct caaaaccgcg ttcaaggatt tccagattcg taagaacttc agattccttg
    19081 acgtaatttg gcgcaccgtt ggtgatggtt tgatacaacg catcataaac gcgcccataa
    19141 tcgcccatct ctggcttcat ctcttctctt accgtcacgc cctcgtcatt gacatactcc
    19201 agcacaccga ccgaatcatc cgctgcgaat cctggttcgc ccggcataat attagccttc
    19261 aggctggttt cctgctggtc gataccgtat ttaataaacg aacctttctt accgtgaacg
    19321 ataaatttcg gataatcgat tttcaccaga tggctggttt tgacgatggc ttttagatcg
    19381 ccataaaaca gctgcgcttc aaaggtgtcg tccggattgg ctttattacg caggctgcgg
    19441 atgtcataag cgacgtgatc cgggcgaccg aacagagaaa taatctggtc catcgtatgc
    19501 acaccaaggc catagaacgc gccatcctgc ggcagccccg gtttggtttc tgccaccggg
    19561 cggtaatagt caaaatggct ttccacttcg acaatctctc ccagcttgcc gctttcaatc
    19621 gcttttttcg ctgtcaggaa gcaggaatca aagcgacgat tctgatacgg cgtaacgatc
    19681 agccctttgc ttttcgccag tgcaaacagc tctttcgcct gcgcaattgt cggagtgaac
    19741 ggtttttcga ccagcacatt tttcccggct tccagcgcgc gcttcgcgta ctcgaagtgg
    19801 ctgtccgcgt gggtgcagac gacaaccagc ttaacatcgg gatcgtttag cacttcgtcg
    19861 agatcgctgg tgaaatggat atgggaataa atgggggcct gttcttccgg cttcgcatgg
    19921 cggcgaaaaa tatgcgcgac atgccagcta tccttgcggt taagtacata cggcagatgg
    19981 taacgggtgg tgcttttgcc gaagccaata aaggcgcagt taatgaccat gatttcgtcc
    20041 tttttaaggt ggttatttac accttagcgc aaagcggacg tggttcctac tggagtgcgc
    20101 ataacgaaca caagcacttc ccgaggataa attgagaacg aaaggtcaaa aaaaaagcca
    20161 gcacccggct ggctaagtaa tactggaagc aatgtgagca atgtcgtgct ttcaggttct
    20221 ccgcgagggt cttcctgatc gcgagacaat aataatcatt ctcattcgca cttgtccaac
    20281 actttttgca aaaaaatgca tttgactcgc atttgaaagt caatgatgtt gaaagggaca
    20341 tttaccccaa agaggacaaa ggaaggaatg agtgagatag taatacgcca cgcagaaaca
    20401 cgcgatcacg aggccatcag gcagattcac gcccagccgg aggtgtatta caacacgcta
    20461 caggtgcctc atccttcaga acaaatgtgg ctggaacgac tcaccgctcg ccctggcatc
    20521 aagcagttag tcgcctgtat tgatggaatc gtcgtaggcc atctcaccat tgacgtgcaa
    20581 caacgcccac gccgcagtca tgttgccgat tttggtatct gtgtcgactc ccgttggaag
    20641 aaccgcggcg tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg
    20701 cgggtagatc gtattgagct aacggtgttc gtcgataacg cgccagcaat taaggtctat
    20761 aaaaaatttg gctttgagat tgaagggact ggcaagaagt acgcgctgcg taatggtgaa
    20821 tatgtcgatg cgtattatat ggcgcgggtg aagtaagata gtgccctttt tctgagatgg
    20881 aaaaagggtg tcattcaaaa tcggcatacc ttcctttaag gtatttattt tcccaataaa
    20941 tatatgagat t
//
LOCUS       DAZFRG010000066        20498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFRG010000066 DAZFRG010000000
VERSION     DAZFRG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.066"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..685)
                     /gene="yncG"
                     /locus_tag="ACHKUC_001907"
                     /pseudo
     CDS             complement(85..685)
                     /gene="yncG"
                     /locus_tag="ACHKUC_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            952..2451
                     /gene="ansP"
                     /locus_tag="ACHKUC_001908"
     CDS             952..2451
                     /gene="ansP"
                     /locus_tag="ACHKUC_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO6618171.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETSS"
     gene            complement(2564..3625)
                     /gene="yncE"
                     /locus_tag="ACHKUC_001909"
     CDS             complement(2564..3625)
                     /gene="yncE"
                     /locus_tag="ACHKUC_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO6618172.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTNNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3867..5969
                     /gene="pqqU"
                     /locus_tag="ACHKUC_001910"
     CDS             3867..5969
                     /gene="pqqU"
                     /locus_tag="ACHKUC_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO6618173.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNSDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRDFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGQNYGVGINIAWRFE"
     gene            complement(6046..6201)
                     /locus_tag="ACHKUC_001911"
                     /pseudo
     CDS             complement(6046..6201)
                     /locus_tag="ACHKUC_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(6201..6269)
                     /locus_tag="ACHKUC_001912"
                     /pseudo
     CDS             complement(6201..6269)
                     /locus_tag="ACHKUC_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6450..6968
                     /gene="mddA"
                     /locus_tag="ACHKUC_001913"
     CDS             6450..6968
                     /gene="mddA"
                     /locus_tag="ACHKUC_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO6618174.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            6965..7414
                     /gene="ydcZ"
                     /locus_tag="ACHKUC_001914"
     CDS             6965..7414
                     /gene="ydcZ"
                     /locus_tag="ACHKUC_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6618175.1"
                     /translation="MSQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(7415..7648)
                     /gene="ydcY"
                     /locus_tag="ACHKUC_001915"
     CDS             complement(7415..7648)
                     /gene="ydcY"
                     /locus_tag="ACHKUC_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO6618176.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(7734..7907)
                     /gene="ortT"
                     /locus_tag="ACHKUC_001916"
     CDS             complement(7734..7907)
                     /gene="ortT"
                     /locus_tag="ACHKUC_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO6618177.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            8103..8198
                     /gene="yncL"
                     /locus_tag="ACHKUC_001917"
     CDS             8103..8198
                     /gene="yncL"
                     /locus_tag="ACHKUC_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO6618178.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(8295..9719)
                     /gene="patD"
                     /locus_tag="ACHKUC_001918"
     CDS             complement(8295..9719)
                     /gene="patD"
                     /locus_tag="ACHKUC_001918"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO6618179.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCPNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEVVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(9741..10535)
                     /gene="ydcV"
                     /locus_tag="ACHKUC_001919"
     CDS             complement(9741..10535)
                     /gene="ydcV"
                     /locus_tag="ACHKUC_001919"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6618180.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(10525..11466)
                     /gene="ydcU"
                     /locus_tag="ACHKUC_001920"
     CDS             complement(10525..11466)
                     /gene="ydcU"
                     /locus_tag="ACHKUC_001920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6618181.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(11467..12480)
                     /gene="ydcT"
                     /locus_tag="ACHKUC_001921"
     CDS             complement(11467..12480)
                     /gene="ydcT"
                     /locus_tag="ACHKUC_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6618182.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            complement(12498..13644)
                     /gene="ydcS"
                     /locus_tag="ACHKUC_001922"
                     /pseudo
     CDS             complement(12498..13644)
                     /gene="ydcS"
                     /locus_tag="ACHKUC_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
     gene            complement(13889..15295)
                     /gene="ydcR"
                     /locus_tag="ACHKUC_001923"
     CDS             complement(13889..15295)
                     /gene="ydcR"
                     /locus_tag="ACHKUC_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6618183.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(15374..15610)
                     /gene="hicB"
                     /locus_tag="ACHKUC_001924"
                     /pseudo
     CDS             complement(15374..15610)
                     /gene="hicB"
                     /locus_tag="ACHKUC_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            15789..16019
                     /gene="yncJ"
                     /locus_tag="ACHKUC_001925"
     CDS             15789..16019
                     /gene="yncJ"
                     /locus_tag="ACHKUC_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO6618184.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(16111..18072)
                     /gene="rlhA"
                     /locus_tag="ACHKUC_001926"
                     /pseudo
     CDS             complement(16111..18072)
                     /gene="rlhA"
                     /locus_tag="ACHKUC_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
     gene            complement(18145..18681)
                     /gene="sutR"
                     /locus_tag="ACHKUC_001927"
     CDS             complement(18145..18681)
                     /gene="sutR"
                     /locus_tag="ACHKUC_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO6618185.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVFVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            18755..19933
                     /gene="ydcO"
                     /locus_tag="ACHKUC_001928"
     CDS             18755..19933
                     /gene="ydcO"
                     /locus_tag="ACHKUC_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO6618186.1"
                     /translation="MTHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGA
                     TTAQISGWMTALGLAMGVSTLTLTLCVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVT
                     NVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLAW
                     LATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPDFSFAHSLSVAL
                     PLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAI
                     CQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTI
                     GGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCYVVLNLIADRNR
                     "
     gene            complement(19968..>20498)
                     /locus_tag="ACHKUC_001929"
     CDS             complement(19968..>20498)
                     /locus_tag="ACHKUC_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO6618187.1"
                     /translation="ATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVL
                     DKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVV
                     AGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQV
                     IDDMATDATMFDVNKK"
BASE COUNT         4943 a         5247 c         5111 g         5197 t
ORIGIN      
        1 acattaattg ctattcatag caaacaaatg gacgtccagt taactacgcc agtgattcat
       61 taacattaac ctccggcatt attgttaaat aatgacattc ctttttaata cttcttgtaa
      121 tttcggtatc tgacatacag catcagcaat cgcattaatg ttcggggcat tatcctgaaa
      181 ccattcgtgt cccggtcccc atgtgcgcat ggtgcagaga taacagtcca ccagcgttag
      241 ttgcactcca aacacatacg gttcagcagt caattgcgaa ttcagccaga tataaagcga
      301 tttgcgatat tcaattacag gtgcgtcaga tgcccaacgc ttagggtaat cggcgaaagt
      361 gaacgttgga tagacattag caaccagcca gaccaatagc cgttgaaact gttgacgttc
      421 ggcgcgccca agaggtggcg caagatccgg gcgacgatcg agaaccatca atgcaatcgc
      481 cgctgtctcc gtcataatct cgttattttt acagcgccag tgtcgggacc tgacacaacg
      541 gattcagggt ttttaataaa tcgcgtgaag ttccctcgtg gtcaaaacca ctgacatcaa
      601 cgaactgata aggaatatca gccagtgtta gcatcagctc actgattgta gagccccagc
      661 cgggtacgcc atacacctta atcatatgcc cctcacaatc ttttaaagat taagtgtagg
      721 acagcacaca ggttatccag caaaaggatt tatcattttt tcgtcagttg tataaatccc
      781 cttcaggatt gtaaggggat gttttgcatc gtttatcgat aacgggtagg atgctgcgcc
      841 gatagtttta tacccataaa gaataatggt gataactatc atcgccagga tgaataaaca
      901 ttgttcatgg caacttatat gactttttca ttaaagcaat cagggagagc aatgagtaaa
      961 cacaacaccg acacttcaga tcaacacgcc gcgaaacgtc gctggcttaa tgcccacgaa
     1021 gaggggtatc acaaagcgat gggcaatcgc caggtacaga tgatcgccat tggcggcgcg
     1081 attggtacgg gtttgttttt aggcgcgggg gcgcgactgc aaatggcggg gcccgcactg
     1141 gcactggtct atttgatctg cggcctgttt tcgtttttta ttctgcgtgc attgggtgag
     1201 ctggtgttac accgcccttc cagtggcagt tttgtctctt atgcccgtga gtttttgggt
     1261 gagaaggccg cttatgttgc tggctggatg tacttcatca actgggcgat gacggggatt
     1321 gttgatatta ccgccgtcgc tctgtatatg cattactggg gcgcgtttgg cggcgtgccg
     1381 cagtgggtct ttgcgctcgc tgcactgacc atcgttggca ccatgaatat gatcggtgtg
     1441 aaatggtttg cggagatgga gttctggttt gcgcttatta aagtgctcgc cattgtaacc
     1501 tttttggtcg tgggtacagt gttcctcggt agtggtcagc cgctggatgg caacaccact
     1561 ggctttcatt taatcaccga taatggcggc ttcttccccc acggtttgct gcctgcgctg
     1621 gtgttgattc agggcgtagt gtttgctttt gcctccattg aaatggtggg tacagcggcc
     1681 ggagaatgta aagatcccca gaccatggtg cctaaagcga ttaacagtgt gatttggcgt
     1741 attggtctgt tttacgtcgg ctccgtggtg ttgctggtta tgttattgcc gtggagcgcg
     1801 tatcaggcgg ggcaaagtcc gttcgtgacg tttttctcta aactgggtgt gccgtacatc
     1861 ggcagcatta tgaacattgt ggtgctgacc gctgccctct ccagcctgaa ttcaggtctg
     1921 tactgcaccg ggcgtattct gcgctcgatg gcgatgggcg gttccgcacc gagttttatg
     1981 gcgaaaatga gtcgtcagca tgtgccgtat gccgggattc tggcgacact ggttgtgtat
     2041 gtcgtcggcg tattcctcaa ctatctggtg ccgtcacgcg tatttgagat tgtgttgaac
     2101 ttcgcgtcgc tgggaatcat cgcttcatgg gcgtttatca tcgtttgcca gatgcgcctg
     2161 cgtaaagcga ttaaagaagg caaagcagcg gatgtcagtt ttaaactgcc tggcgcgccc
     2221 ttcacttcct ggctgacatt actgttttta ctgagtgtcc tggtgctgat ggcgttcgat
     2281 tacccgaacg ggacttatac catcgcggca ctgccgatta tcggtattct gctggttata
     2341 ggctggtttg gtgtgcgcaa acgcgttgct gaaattcaca gcactgcgcc agtcgtcgag
     2401 gaagatgaag aaaaacagga aattgtgttt aagcctgaaa cgtcgagtta attgcaaaat
     2461 tgcccggcta cgtgccgggc aaattcagac tactgacgaa ggtgccggat gcggcgtgat
     2521 aaaagtcagg caattctttc cgttttcacc ctcgccatat ctattacagc gcaatacgaa
     2581 tcacatcgtc cggctgggta gcttcctgct gtttagtgga tttttgtttc acactgacat
     2641 acagcgtttt gccatcggca gacagcgcca ggctgttcgg atgagtcggc gtatcgaacg
     2701 ttttcaccac tttatagctt ttcgcgtcaa tcacactgac tttacctgcc tgacgatgcg
     2761 tcacgtaggc ttcattacgc gctgggttaa acagcacagc cagtgattcc ggtgccgcaa
     2821 ccttcgccag aatattgcca ttacgggtat cgaccactaa cacttctgcc gctttagaat
     2881 cggtgataaa tgcacgctga ttgttggtat caaggctgat gttgataaag aagtgctctt
     2941 tgccgtcatc cagcagcttt ttacggctga ggattttatt gtcggcggtg tcgatggtaa
     3001 tcaattcgcc gtcagcgtta gtggtgtaaa gacgtttgcc tttgctatcc agcgccagac
     3061 cggtactcat tttaccggtg ttctggatgg cggttttcag tttgatattc tcgccatcaa
     3121 cgacccaaat cacgctctct ttaccaatac cactgatgta aacggtgttc gtggcatcat
     3181 cagctaccag ctcacgtggt tgcaacgggc gtacctcttc cgtgcgctta cgatcatcca
     3241 gcaccagacg acctttcact tcgcccgttt tggcatctat cgccgtgacc gcactattta
     3301 cggtgttacc aaaccacaac gtctgagtcg tgttattgat ggtggcacca aacggcttga
     3361 gatcgttatg gatcgcctgc gtcacttcca gagtaaccgg atcaagacga taaaccacgc
     3421 cgcctttatc cagtttgcgg ctttgcgaag tggcaagcca cagcgcgttt tcttgctggc
     3481 tataagccat ttcgtaggca cctttaccta ccgctttacg cagcatttct tctgcggcct
     3541 gcgtactgaa tgatgaagca acaagcaatg aacctaacag taatgaacca cgcaggcgcg
     3601 atgaaaacag atgacgtaaa tgcatgacga ctccctttga tgaaccgata tttttgctgc
     3661 ttcacaatgc ttcgctaagc gtaatcatcg ttacgctctt gttataaatg ggaatggtaa
     3721 tcattatttt ctcaaatgtg gagcatttct taccgttacg actacaattt tctcaattac
     3781 ttaacgtatt gtgccgtttc gttttccata aatttacaat ttcattacca tgacggtgtt
     3841 ttcccattct taacctggct tttttcatga agattttttc cgtccgacag accgttttgc
     3901 ccgcactgct tgtcctttcc cccgttgttt ttgccgctga tgaacagacc atgattgtca
     3961 gtgccgcacc gcaggtggtt tcagaactgg ataccccagc agccgtgagc gtggtggatg
     4021 gcgaggagat gcgcctggca acaccgcgca ttaacttgtc tgaatcactg accggcgtgc
     4081 ctggtttaca ggtacaaaac cggcagaact atgcgcaaga tttacagctg tcgattcgcg
     4141 gatttggctc ccgctccact tacggaattc gtggtattcg cctgtatgtg gacggtattc
     4201 ccgccaccat gcccgacgga caagggcaga catccaacat cgatttaagc agtgtgcaaa
     4261 atgtggaagt gctgcgtggc cccttctctg ctctgtatgg caacgcgtct ggcggtgtaa
     4321 tgaatgtcat cacccagacc ggacaacagc caccaaccat tgaagccagt agttactacg
     4381 gcagttttgg cagctggcgc tatgggctga aagcaacagg cgcaacgggc gacggcacac
     4441 agcctggcga tgtcgattac accgtctcaa ccacacgttt tacgacccac ggctatcgtg
     4501 accatagcgg cgcacagaaa aatttagcca atgccaaact gggcgtacgc attgatgacg
     4561 ccagcaaatt aagcctgatt ttcaatagtg tggatatcaa agcagatgac ccaggtgggc
     4621 taaccaaagc agaatggaag gcgaatccac aacaagcgcc tcgtgcagaa cagtacgaca
     4681 cgcgaaaaac catcaagcaa actcaggctg ggttgcgcta tgagcgtagc ctgagcgcgc
     4741 aagatgatat gagtgtgatg atgtatgccg gagagcgaga aacgacccag taccagtcaa
     4801 tcccgatggc tccccaactt aacccgtcac atgcgggcgg cgtgattact ctgcaacgcc
     4861 attatcaggg gatagacagc cgctggacac accgtggtga actgggcgtt ccagtcacgt
     4921 tcacaaccgg cctgaactac gaaaacatga gtgaaaaccg caagggttac aataacttcc
     4981 gcctgaatcg cggcgtgccg gaatacgggc aaaaaggtga gttgcgccgc gacgaacgca
     5041 atttgatgtg gaacgtcgat ccctatttac aaacccagtg gcaactgacc gacaaacttt
     5101 cgctggatgc tggcgtgcgc tacagctccg tgtggtttga ttccaatgat cattacgtta
     5161 ctccgggtaa cagcgacgac agcggtgatg ccagttatca caaatggcta ccagccggat
     5221 cgttaaaata tgcaatgacc gatgcctgga atgtctatct ggcagccggg cgcgattttg
     5281 aaacgccgac gattaatgag ctgtcttatc gcgctgatgg gcaaagcggt atgaactttg
     5341 gtttaaaacc ctccaccaac gatacaattg agatcggcag taaaacgcgt attggtgatg
     5401 ggctgcttag tctcgcattg ttccagaccg acaccgatga tgaaattgtt gtcgatagca
     5461 gtagcggtgg gcgtacgact tacaaaaatg ccggaaagac ccgtcgtcaa ggcgctgaac
     5521 tggcatggga tcaacgtttc gcgggagatt ttcgcgtaaa agcgtcctgg acctggcttg
     5581 atgcgaccta tcgcagcaat gtttgcaatg aacaggattg taacggtaac cggatgccag
     5641 ggatcgcccg taatatgggc tttgcgtcga taggttatat accggaagat ggttggtatg
     5701 caggcacgga agcgcgttat atgggcgata ttatggcaga tgatgaaaat acggccaaag
     5761 cgccttctta tacactcgtc ggcttattca ccgggtataa atacaattac cacaatttaa
     5821 ccgtggattt atttggtcgt gtcgataatt tattcgataa agaatacgtt ggttctgtca
     5881 ttgtcaatga gtcaaacggg cgatattacg aacctgcgcc cggacaaaat tatggtgtcg
     5941 gcataaatat tgcgtggcga tttgagtaat tatctttaag cccggtattt gccgggcgta
     6001 ttttatttat cggctgtatt gctggcacaa catagcaacg tttttttgca tagtgaggtt
     6061 ttcgacagcc agagcaactg ccattaattc cagttcgtag cggatccgat tgatttcatt
     6121 taattgacgt tttcccactt ccggaactgt aattgcttgc gctggtgcga caaagagcgc
     6181 atttatcgaa accagacgta aggaaccggc gcgccatgcg gacgcgaggc cagaacccaa
     6241 catcggttac gctgcgtttg ttgccccata atgctctcct gtgcttttgg catgaatcca
     6301 tcaatagtag caggctcaca ggattggttt tgtttgcagc cgtcacatcc cctctgcaac
     6361 acgcaataat ttcatagagg aatccgttgc ttcgggctgt tttcgatagt attgataaca
     6421 tcatcatcat tatttaatcg aggccagaca tgtccatccg ttttgcccgc aaagccgact
     6481 gtgctgcaat tgcggaaatt tataaccacg ccgtgttgta tacggcggct atctggaatg
     6541 accagacggt ggatgcggat aaccgcattg cctggtttga agcgcggacc atagcaggtt
     6601 atccggtgct ggtgagtgaa gaagatggcg tagtgacggg atatgcctcg tttggcgact
     6661 ggcgtagttt tgatggtttt cgccataccg tggaacattc ggtttatgtc catcccgatc
     6721 atcaggggaa aggtctgggg cgtaaattgt taagccgatt gattgatgaa gcgcgggatt
     6781 gcgggaagca tgtcatggtc gccgggatcg aatcgcaaaa tcaggcctcg ctgcatctgc
     6841 accagtcgct gggatttgtc gtcaccgcgc aaatgccgca ggtaggcact aaatttgggc
     6901 gctggctgga tctgacattt atgcagttgc aactcgacga gcgcactgaa ccggacgcga
     6961 ttggatgagt cagtcactta cccttgcctt tttgattgca gccgggattg gtctggtagt
     7021 ccagaacacg ctgatggtgc gcatcaccca gacctcctct accattctca tcgccatgtt
     7081 gctgaactca ctggtgggga ttgtgctctt tgtttctatt ttatggttta aacaaggcat
     7141 ggcggggttt ggcgaactgg tgtccagcgt gcgctggtgg acacttatac ctggcttgct
     7201 gggatcgttt tttgtcttcg ccagtatcag cggatatcag aatgtgggag cggcgacaac
     7261 tattgcagta ctggtcgcca gccagctgat tggcggtctg gtgctggata tcttccgcag
     7321 ccatggcgta ccacttcggg cgttgatcgg cccaatttgt ggcgcaattt tacttgtggt
     7381 tggcgcctgg ctggtggcca gacgctcatt ttaatcagag gatggtaccg cctttggtta
     7441 actgttcgtg gcgcgcttcc atatcttctt tatgtttgcg accgtgatga gcaatagcgg
     7501 tgcgcaaacg ctgctgctgg gtgtagcgat cttcccgact taactccgcg tcatcgctca
     7561 gggcgattaa taattcgttc atatgggcaa tgacgctctc ttcaatcgcg gcatccacgc
     7621 gcgcgatgac ttcatccaga tgcgacatac tttctcctta gccacttgtg aaatttatcg
     7681 attcatccaa atgatataag tctgcgtggt gccgtcaagc cagtgcataa gatttaaaac
     7741 agagaaaaca acagcgccac cgggaaactc atcggccatg ttgcccccac cagaaaggcg
     7801 cttaagaaac ggatgcgttt cttatcgtga gaaagaaacc aggtgataag aaatgcgatt
     7861 gctgccataa ccgcataaaa aacaagcata tgttgataga gagacatgta cgagcgtcca
     7921 gccagatgaa taaaacgcgc gcaaatatgc gtcatttttt tgacgtgcat caagtttttt
     7981 caccagttag agaaaaaatc atcaaacagc aacttgccct tatctatctt cagaatgagt
     8041 attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa
     8101 aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt
     8161 ttactcttat ctctatgaga tttggctggt ttatttgatg ttgtgatttt gcacgcagca
     8221 cgtactgtca gagaaatcag tgctctgaca gtacgcaaga tcatctcata ctcttacgat
     8281 ctgcaatatt atccttaatg tttaaccatg acgtggcgga cgacggtgta atcctccagc
     8341 ccataaagtg acatatcctt gccgtaacca gaaagtttct gcccaccgtg cggcatttca
     8401 cttaccagca tgaaatgggt attgacccag gtacaaccat attgcagccg cgcgctgacg
     8461 cgatgcgccc tgcccacatc cttcgtccat accgaagatg caagtccgta ctgactgtca
     8521 ttcgcccagt tcaccacctg ttcttcgttg tcgaagagcg taacactcac taccggacca
     8581 aatacctctt tttgcacgat ggcatcgtcc tgtaatgctc cagccagcag cgtcggcgca
     8641 tagtaatagc cattaccctt gcgcttttca ccgccagtga tcactttgat gtgccctgtc
     8701 gctttcgcct cttctaccgc cttgctgacg cgttcgagat gcgccagcga gcttaaaggt
     8761 ccaagctccg tagactcgtc atccggcgaa cccgatttta acgttgccac cgcagcaccg
     8821 agtttttcca ccagcgtatc gtaaatgcct ttttgcgcgt agatccgaca agccgcagta
     8881 caatcctgtc cggcattgta atagccaaaa gtacgtacac cttcgaccac tacttcaata
     8941 tccgcatcat caaaaacaat cactggcgct ttgccaccaa gttccatatg agtacgctta
     9001 atggacggcg cggtatggct gatgatgtgt tccccagtgg cgatagaacc cgtcagcgac
     9061 accatccgca ctttgggatg tccggtcagc ggatctccca ccgttttgcc tctgccaaac
     9121 agcacattaa tcacgccagc cgggaagata tctttcgcca gctctgccaa cttcaacgcg
     9181 gtcagcgggg taatttctga tggttttagc actacgcagt tccctgccgc cagcgccgga
     9241 gccagtttcc acgcagccat catcagcgga taattccacg gtgcgataga agccacgacc
     9301 cccaacggat cgcgacggat catcgaagta tgaccttcaa gatattcacc tgccgccaga
     9361 ccattcgggc agcgcgccgc accggcgaaa aagcgaaaga catcgacaat cgccggaatt
     9421 tcatcattga acgcactatg cagcggtttg ccacaattac gggactccag ttcggcaaaa
     9481 acctgaccat tttcttcgat aacatcagcc agtttcagca ggcattccgc acgcgctttt
     9541 ggcgtggttt gcccccattc ggcaaatgct gcatctgccg cgcgaacagc agcattgacc
     9601 tgctctgcgg atgcctcggc aatctccagt aaaacgtccc ccgttgccgg attatagaca
     9661 ggctgttttt ccccttcgcc gctaaccagt tctccgttaa tcagtaactt atgttgcata
     9721 gcatattcct gtatcagtgg ttattgacca ttgtcgcctt cgcgggttag ccaccaggcc
     9781 cccaggatcg gcaaggttgt taccaacata accagcagtg ccaccacgtt ggttaccggt
     9841 acatcacgcg gtcgcccaag ctgattgagc aaccacaacg gtaacgttcg ttcatgacct
     9901 gccgtaaaag tcgtcacgat gatttcatcg aacgacaagg caaacgccag cattcctcca
     9961 gccagtaacg ccgaactgag attcggcaac actacgtagc ggaaggtttg ccagccattg
    10021 gccccaagat ccattgacgc ctcaaccaga ctccacgagg tgcggcgaaa acgggcgatg
    10081 acattgttaa acaccaccac tacgcaaaaa gtcgcatgac cgaccacgat ggtgaaaaat
    10141 cccggctcca gattgatggt tttaaaggcg gttaatagcg ccagaccagt gacaatgccc
    10201 ggcagcgcaa tgggcagcaa cagtaacagc gaaatggcgt ttttgccgaa aaagtctcgt
    10261 cgccacagcg cggcagcggc cagcgtccct aacaccagcg cgattaatgt cgccagcgcc
    10321 gccactttaa gtgacagcgt cacggcatca agaatatcac tacgctgtgc tgccacgcta
    10381 aaccagcgca gcgtcaggcc ttgcggtgga aaactaaacg ccgcatcttc agtgttaaac
    10441 gcataggcag cgataatcag gatgggaaaa tgtaggaaaa ccacgccgcc ccaggccgcc
    10501 agtttgagga aaaacggtgc gcgttcagag tgcatcgaac gctcccagac gtttcacgaa
    10561 cgccaggtaa agtgcgatga gaacaatcgg caccagggtg aatgccgccg ccatcggcat
    10621 attgccaatc gccccctgct gggaataaac catattgccg ataaaatatc ctggagggcc
    10681 aaccagctgc gggacaataa aatcgcccag cgtgagtgag aaggtaaaga tagagccagc
    10741 ggcaataccc gggattgcca gcggcaacac cacatagcga aaggtttgtc gtggacgtgc
    10801 gccaagatca gccgacgcct gcaacaatga cggcggcaaa cgctcaagcg ccgcctgcac
    10861 gggcaggatc atgaacggca gccagatata gagaaacacc agaaagcgcc ccagcccgga
    10921 agttgacagc gtattacctc ccaccgccgg tagcgtgagg aacgcagtca gcagtggttc
    10981 cagcccaaga tgttgtaaaa accactgagc cacgccatct tttgccagca ataacgtcca
    11041 ggcataggct ttaacaatgt agctcgccca catcggcaac attaccgcaa tataaaaaaa
    11101 cgctttcatt tttccgctgg tatagcgcgc catataccac gccattggaa aagccagaat
    11161 ggcgctggcg atagtgactg ccacagccat ggtcagcgtg cggacaatga tgtcgtaatt
    11221 cgccggatta aacagcgcac ggatattcgc cagcgtcagt tccggcgtta ccgacatggt
    11281 gaaatcgtca aaggtataaa agccctgcca taacagcgtc agcagcgagc cgaaatagac
    11341 aatgccaaac cacattagcg ggccaagcag cagcaaaaac agccccagcc ccggattacg
    11401 ccagaagaaa ccggacacct tacccagacc tggacgtgaa ggtgattgca atacgttcat
    11461 cgccattcac ctctcctcaa ccagcgccac catcacatca cgcgaccagg aaaccatcac
    11521 ctgttgtccg ggcgtgagcg tggcaggcag ttcttcgcct gtcatattgg cctgactcac
    11581 cagcagtttt tcaccgccgc tcagtgtcag ttcaaaacgg gtcgctgcgc cctgatattg
    11641 caccgcctgg atcgtgccat tagcctgcat ttcgccaggg gtgttgaggc ggatatgttc
    11701 cggtcgtagt gcgaagcttc ccgtcatgcc acaaagtttc tctgccatca gtccatcaaa
    11761 aacattcgat gtcccaacga atcccgcaac aaacggcgtg cgcgggcgca tatagagatc
    11821 gcgcggagaa tcgacctgtt caatgcgtcc attattgaaa accgccaccc gatcggacat
    11881 cgataacgct tcgccctgat cgtgggtaac gaagataaaa gtgataccga gagactgttg
    11941 cagctttttc agctcaagct gcatttgctc tcgcaatttg agatccagcg cgccgagcgg
    12001 ttcatccagc aacaacacac gcggttcgtt caccagtgct ctggcgatgg cgactcgctg
    12061 gcgctgacca ccagaaagtt gtgacggttt acgttgctgt acaaacccca acgccacttt
    12121 ctcaagcgcc tcttgcgcca ttgcatgccg ctgcttttta ttcacgcctt tgaccatcag
    12181 cccgtaggct acgttgtcaa gaatcgacat atgcggaaat agcgcgtagt cctggaagac
    12241 agtattcacg tcccgctccc acggtggcag attgctggcg ggcgtaccaa aaatagagat
    12301 agccccgccg gaaagctgtt cgaatccagc aatcaggcgc aggcaagtgg ttttgccgga
    12361 gccggacggc cccagcatag agaagaactc accatctttt atcgcaatac tgacgccatc
    12421 tactgcgcgc acgtcaccgt acaaccgtga gacgttgtca aactccactg cgtacgtcat
    12481 aaaacacccc agcgaagtta gcgaccgccc ataatggcaa tgtaatcctg cgtccagcga
    12541 ctgtagggaa caaacttgcc cccttctgct ataggcgttt tccagaaggc gattttatcg
    12601 aaatagttaa aaccgtttgt ttcacaacct ttctcgccta ataacgcgac tggctttaca
    12661 cccttgcggc actactggta acgagccaaa ccaggccgcc acatcgccct gcacttttgg
    12721 ggttaatgac cagttcatcc atttgtaggc gcaaaccgga tgtttcgctt cgctatgtag
    12781 catggtggtg tcagcccaac cggtaacgcc ctccttcggg aaaacggtag cgacaggctg
    12841 gccttcggct ttcagggcgt tggcctgata gggccatgca ctggaagcaa ccacaccctc
    12901 gtttttgaaa tcgctcattt gcacggtagt gtcatgccag tagcgatgga tcaaattatg
    12961 ttgatcgcgc agcactttca gcaccgcctg atactgttct tcggtgagtt gatacggatc
    13021 actgatgccc aactgtggct gcgtggcttt gacgaacaac gcggcatccg caatgtagat
    13081 agggccatca taagcctgta cccggccttt attgctcttg ccgtccggca gattctgctc
    13141 aacaaaaacc acttgccagc tatccggcgg cgtcgggaag gttttagtgt tgtacatcag
    13201 caggttcggc ccccattggt aaggtgtgcc gtaaactttg ccaccaacgt taaaccagtc
    13261 gcctttaacc acgcgcggat cgagcgcttt ccagttggga atcaaggcgg tattaattgg
    13321 ctgcacgcgt ttgcccataa tcaaacgcag cgaggcatcg ccggatgccg taaccagatc
    13381 gtaaccccct ttggccatca gactgaccat ttcatcggaa gtcgcggcgg ttttaacatt
    13441 caccgcgcag cctgtctctt tttcgaattg cgttacccag tcgtactgtt tatcagtttg
    13501 tccgcgttcg atgtatcccg gccaggcgat aatatccagt cgcccttccg gattctctaa
    13561 attggtaggc ggttcggcgg cgtgagcggt cattattgtc atgctgagcg cacacaggct
    13621 gctgcgggca aatgtcttgc tcataaggtc ttactcctgt cgtaattaat tgttcagccg
    13681 ccgtgccgta aaaatatctc cttgtttaac catagacggc agcttgcagg cacaccttcg
    13741 gccctgagaa aatttaatca ggttgtgagc taccgcgcat tatgccgtct gtaaggttat
    13801 cttctggagt atagacaagg agttagaata tatgactgct attcgaaatt catattaccc
    13861 aacctgagag gaattaaatt aaacgctatt acagccgttc ttgaataagt ttgcctaatt
    13921 gttttaccgc ctgttcttca cgttctcccc actgccacgc ggtattaaaa cgaaaaaaac
    13981 gtgaccagtt ttcaccggta gaaaacatat tacccggcgc aatactgata tgatgcgtca
    14041 gtgccaccag gcttaattct cctgcatcta acggctcggg gagctccagc cagagaaaat
    14101 aaccgctgtc attgtgatga attttgactt ccgcaggcag ataacgcagc aaagcctgcc
    14161 aggcgcgttg tttacgttcc gcaagctggc gacgcaggcg acgaagatga gcatcgtatc
    14221 ggcgagtaga aaggtaatcc accagcgcaa gctgcatcgg tgagctggtg gaaagcgtac
    14281 tcatcaactg caagcgttga attttacgtg catgttttcc ggcggcgacc caaccaatac
    14341 gaaaaccagg caccagacat ttcgaaaacg aagagcaatg caaaacacca tcgtggcgat
    14401 cccacgcttt cgcaggcagc ggtttttccc gtccaaaata aagttcgcta taaacgtcat
    14461 cttcaatcag cgttacgttg tactgattga gcaacgctac cagccgcact tttttctgcg
    14521 gcgttaaggt aaatccaagt gggttctggc tattagtcat cagccagcac gctttcaccg
    14581 gatactcctg caacgccagt tccagcgcct gaagatcgat cccttcttta atatccgtcg
    14641 ctaccgataa cgccttcagc cgtaaccgct ccagcgcctg caacgcgccg tagaaacaag
    14701 gattctctac tatcacccaa tcgcccggtt cagttaccgc ttgcaaactg aggtttaatg
    14761 cctctaacgc cccggcggta atgacaattt catcaggaga aatggtgatg ccctgtaagg
    14821 catagcgacg agcaatagcc tgacgcagtt ctgcgtttcc tggcggtaag ttttcaatca
    14881 cgctcatcgc cgtggcggtt ttgcttacct gcgccagcga gcggtttagt tgttggaggg
    14941 ggaaaagtcg cgggtcggga aaggccgagg caaacggaac taccgacgga tcacgactgg
    15001 cctgcaacat atcaaaaata taggtgttga tatcgacggc ttcatctcgt gtgactggaa
    15061 tgactgtcgc tttcggcatt tttattgcct gtggcgcaac gtaataaccc gattgcggtc
    15121 gcgcgataat atatccctga ctttcgagca actgataggc atggctgaca gtcataaagc
    15181 tcatgccaga aagcgccacc tggtcacgca acgaaggcaa acgatcgccg ggttgccaga
    15241 tacccgacgc aatctgctcg cgtaattgtt ctgcaagctg ctggtatttt ttcattatcg
    15301 aatcgttatt atgtgcaggt gattcggcag tctatatcag ttgttaaaaa tgacaacttt
    15361 tcattaactg ttatcaaacc atcaccagcg ataactcttt gcctagcgca ttagccgcga
    15421 gctggacggc gtcgattttt gtcgcatggt gcaaattaaa taggcgagta atctcctgtt
    15481 tatgtttgcc aattcgcctg gctaactctt gctgagtgat ttctgactgt aaaaaagcat
    15541 ttaacagcaa taccttagag gcgacgctca aaggcacttc aataaagtga tcgtgactat
    15601 ttaatggcga agtttacctc cgtgtgtaat gtgctcgaaa atagcactaa atcgccgcaa
    15661 cggcagaatg caatgcaaaa tcaggaagga agattccaga atttctttgc tctaatttgc
    15721 aaaactgaat aataacctca agaaacgatc ggcatcatag cggcaaaagg ttagaaaaag
    15781 gtggcgggat gttttcgaag gcgttatcgg ttgtcttatt aacgtgtgca ctgttctcag
    15841 gacaactcat ggcagggcac aaaggacatg aatttatgtg ggtaaagaat gtggatcatc
    15901 agctgcgtca tgaagcggac agcgatgaat tgcgtgctgt ggcggaagag tcggcggaag
    15961 gtttgcgcga gcatttttac tggcaaaaat cgcgcaaacc agaagcggga cagcgttgag
    16021 tcgtgtgttg ccggatgcgg catgacgcct tacccggcag gcaatatcat gcagatttga
    16081 tgagtggtga gtaacccgaa aactggcgtt ttaccctttt cgcttcggca atgttttcag
    16141 cagatcatcc ggacttacgg atgccactac gcttcccggt aacggcattt tcagtatgtg
    16201 atttttgatt ttgccaatga cgtgcatctc gcatgggcgg caatcaaact atagcgttaa
    16261 tacttcatcg ccgttaacca gttgcattgg cgtcgccttc cagcttttga tattgccttt
    16321 cgcctgtttc gggcacagat tgaaggcaaa gcgcagacaa tgcttggtga tcatcaccgg
    16381 gacttcgccc ttctcttcat gtgcctcata cgccgcgtca atcagttgca caccatagcg
    16441 atgataaaat tcacgcgctt tctggttgta tacgttcgcg aggaaactca gatgcgtttg
    16501 cggataaacc ggcgcaggat cagcaaccgg tttacggctg ccgcgctggt aactggcaag
    16561 acgcgcagca tcaagcatat cggcggcttc acggcggaac tggtttaaca gactgtttgg
    16621 tacaaacagc gcgccaggca aattaatttg cacattgcgg gcgtaataga tggtttgccc
    16681 cagttttgcc agaccatcct tcagattgtt cattgctttt tcggcgttat tggcttcgtc
    16741 gaactgcccg tccagcgtat gtgtgatgct gacaccctct tcactggtga gggtcagaat
    16801 cagttgttcc tgccagccgc ccagttcaat gtctaccgcc acccggcgtt cgctggaggt
    16861 ttttgtcagt gcctgctgcc agttatgatc aagatttcgg tttagtgggt gatgcgggcg
    16921 aattttgtgc aaatctgctg gcatttcatt gggccagacg cggtactgat tttctccggt
    16981 tttctctacc gtattggcac gaaaaccgac gacttcacgt ttaatcatca cgttcaggcc
    17041 atcgccattt gccagtggct cggtaacggc aacatcgaga tgatctttcg ctactttcaa
    17101 tacttcgccc accggcaggc cgataaattt cggcgaatcg aacgcgccaa tatcgccttt
    17161 acgggcattc acaaaataat ctgtgctacc acggtggaaa gtcttttccg tcgatggaac
    17221 aaagaaatgt tcagtacgac ctgatgaagc gcgcgccaga tcgccacgtt cttcaataat
    17281 ggcatccagc atctgacgat aatgggcggt gatattcttc acgtagctca tatctttgta
    17341 acgcccttca atcttgaagg agcgtacgcc agcatcaatc agcgcgccga ggttggcagt
    17401 ctgatcgtta tctttcatcg acagcagatg tttttcatag gaaaccacac gcccctgatc
    17461 gtctttcaat gtgtatggca aacggcacgc ctgcgagcaa tcgccacggt tggcgctacg
    17521 ccctgtttgc gcatgagaaa tgtagcactg acccgaatag gccacgcaca atgcgccatg
    17581 aataaagaat tcaatggtcg cgtccgtagc ctggtgaatc gcgcggatct gctcaagatt
    17641 cagctctcgc gccagcacaa tctgcgtgaa gccaacatca gagaggaact tcgctttttc
    17701 tactgtacga atatcgcact gcgtactggc gtgcagttca atcggcggaa tatcaagttc
    17761 cagaatcccc atatcctgaa caatcagcgc atcgacaccg gtctggtaga ggtcagtaat
    17821 cagccgttgc gcgggttcca gctcatcatc atgcaaaatg gtgttaagcg tgacgaaaat
    17881 ttttgcacca taacgatggg caaacggcac caactcggca atatctttca ggctattact
    17941 ggcattatga cgggcaccaa aaccagggcc gccgatataa acagcatcgg caccgtgcaa
    18001 aatagcttcg cgggcaatgg cggcatcgcg tgccgggctt aacagttcaa gacgatgaga
    18061 agatacggtc actttcttat ccagtagctg aaaaatggcg gctattttag ccacaagcgc
    18121 gacgttgcga aatagttttc ccgcttaact gcgcgggtaa tggatgaggg aatgaaaatg
    18181 tacggtttgc tccccaccat tacgatagac atgcgtgacg tctgcagcaa atcgaacgcc
    18241 ttccccacaa ttaaggctct gccattcacc atcaacacac agatcaagtt gtccatcaat
    18301 gacgaagaca tgttcgatta cccccttttc atggggcgtc gattcgctta ttgcaccggg
    18361 tgccatctga atcgagaagt gttcaaagca gagttgtgga tcataaggaa acagcgaagt
    18421 gatcaccatc gcctgccgtt gtggatcata aactgaaggt gtagaggact gcggcggaga
    18481 aataaatgtt gaaaatggca cgttcaaccc ggtggcaatt ttccataatg tcgcgaccgt
    18541 cgggctggat tcattacgct caatctgccc taacatcgct ttagaaacgc ccgtcgcttc
    18601 tgccagtcgc gaaagactcc agccgcgctg ctgacgtagt tgtttaagtg tggtggataa
    18661 aaagcgagcg agattttcca tgcgactcct ccggcaaaac ggaagtttat cacttgtgcg
    18721 ttataacgga caaatgctac gttagccgta cgttataacg catgaggtga ctatgcgtct
    18781 gttttctatt cctccaccca cgctactggc ggggtttctg gcggtattaa ttggctacgc
    18841 cagttcagcg gcaataatct ggcaagcagc gattgtcgcc ggagccacca ctgcacaaat
    18901 ctctggctgg atgacggcgc tggggctggc aatgggcgtc agtacgctga ctctgacatt
    18961 atgtgttctc accgcatggt caacgcctgg cgcggctttg ttggtcaccg gattacaggg
    19021 actaacactt aacgaagcca tcggcgtttt tattgtcacc aacgtgttaa tagtcctctg
    19081 cggcataacg ggactctttg ctcgtctgat gcgcattatt ccgcactcgc ttgcggcggc
    19141 aatgcttgcc gggattttat tacgttttgg tttacaggcg tttgccagtc tggatggtca
    19201 atttacgttg tgtggcagta tgttgctggc atggctggca accagggccg ttgcgccgcg
    19261 ctatgcggta attgccgcga tgattattgg gatcgtgatc gttatcgcgc aaggtgacgt
    19321 tgtcacaact gatgttgtct ttaaacccgt tctccccact tatattcccc ctgatttttc
    19381 gtttgctcac agtctgagcg ttgcactccc cctttttctg gtgacgatgg catcgcaaaa
    19441 cgcaccgggt atcgcagcaa tgaaagcagc cgggtattcg gctcctgttt cgccgttgat
    19501 tgtgtttact ggattgctgg cgcttgtttt ttcccctttc ggcgtttatt ccgtcggtat
    19561 tgcggcaatc accgcggcta tttgccaaag cccggaagcg catccggata aagatcaacg
    19621 ttggctggcc gctgccgttg ccggcatttt ttatttgatc gcaggtctgt ttggtagtgc
    19681 cattaccggg atgatggctg ccctgcccgt aagttggatc cagatgctgg caggtcttgc
    19741 gctgttgagt accattggcg gcagtttgta tcaggcgctg cataatgagc gtgagcgcga
    19801 cgcggcggtg gtggcatttc tggtaacggc aagtggattg acgctgttcg ggattggttc
    19861 tgcgttttgg ggattaattg ccggaggcgt ttgttacgtg gtgttgaatt taatcgctga
    19921 cagaaaccga taataaaaag cgccatctaa cgatggcgcg tctggaacta ctttttgtta
    19981 acgtcaaaca tggtggcatc ggtcgccatg tcatcaataa cttgtttaat attttcgaaa
    20041 gccattggcg tactttcgtt attcaggtct ttaccttcgc cctgacgaac gaccttgata
    20101 accggtttat tagtcgctgc atcaatcagc tcaccttcaa aatacaagcg agtatccatg
    20161 gtacggtggc ctgtagccat ttgcgtcccc gcaaccacta atgcaacagg aaccacttca
    20221 tagaattgca gcccttcttt actggtatct acaccggtaa tggccccacg gaaaatcaga
    20281 ctacgcggcc cagcggtggt aaccagtggt ttacgctgcg ctatcgcttc tttcatttcg
    20341 gtgttggtat agttcaaaat tttatccaga actttctgcc ctacctgggt agaaggtttc
    20401 ggtaccggat aataagtgat tgggttccag acgatgctgt catatttgct ttgatcaaaa
    20461 ctcgggtcta cccaacgtaa aacaggttta cctgttgc
//
LOCUS       DAZFRG010000067        20291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFRG010000067 DAZFRG010000000
VERSION     DAZFRG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.067"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..898
                     /gene="pdxI"
                     /locus_tag="ACHKUC_001930"
     CDS             38..898
                     /gene="pdxI"
                     /locus_tag="ACHKUC_001930"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO6618188.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPTEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLNATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            1197..1802
                     /gene="ynbA"
                     /locus_tag="ACHKUC_001931"
     CDS             1197..1802
                     /gene="ynbA"
                     /locus_tag="ACHKUC_001931"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6618189.1"
                     /translation="MTLYQIKPLFQPLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPVSNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            1802..2698
                     /gene="ynbB"
                     /locus_tag="ACHKUC_001932"
     CDS             1802..2698
                     /gene="ynbB"
                     /locus_tag="ACHKUC_001932"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6618190.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVLVGDPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLMGGVITTMI
                     ASLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            2714..4471
                     /gene="ynbC"
                     /locus_tag="ACHKUC_001933"
     CDS             2714..4471
                     /gene="ynbC"
                     /locus_tag="ACHKUC_001933"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO6618191.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLLPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRFIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVAHLHAKGLAVRVVDIAAGHGRYVLDALANEPAISEILLRDYSEVNVAQGQE
                     MIAQRGMSGRARFEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDTGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            4485..5777
                     /gene="ynbD"
                     /locus_tag="ACHKUC_001934"
     CDS             4485..5777
                     /gene="ynbD"
                     /locus_tag="ACHKUC_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO6618192.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGFLNQFTATQDLNNHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFICSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWIVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(5831..6436)
                     /gene="azoR"
                     /locus_tag="ACHKUC_001935"
     CDS             complement(5831..6436)
                     /gene="azoR"
                     /locus_tag="ACHKUC_001935"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO6618193.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            6637..10539
                     /gene="hrpA"
                     /locus_tag="ACHKUC_001936"
     CDS             6637..10539
                     /gene="hrpA"
                     /locus_tag="ACHKUC_001936"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO6618194.1"
                     /translation="MTEQQKLTFTALQQRLDSMMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            10812..11612
                     /gene="ydcF"
                     /locus_tag="ACHKUC_001937"
     CDS             10812..11612
                     /gene="ydcF"
                     /locus_tag="ACHKUC_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO6618195.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            11809..13248
                     /gene="aldA"
                     /locus_tag="ACHKUC_001938"
     CDS             11809..13248
                     /gene="aldA"
                     /locus_tag="ACHKUC_001938"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6618196.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEVVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13290..14291)
                     /gene="gap"
                     /locus_tag="ACHKUC_001939"
     CDS             complement(13290..14291)
                     /gene="gap"
                     /locus_tag="ACHKUC_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6618197.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14480..15010
                     /gene="cybB"
                     /locus_tag="ACHKUC_001940"
     CDS             14480..15010
                     /gene="cybB"
                     /locus_tag="ACHKUC_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO6618198.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKCPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15255..15428
                     /locus_tag="ACHKUC_001941"
     CDS             15255..15428
                     /locus_tag="ACHKUC_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618199.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            15454..15507
                     /locus_tag="ACHKUC_001942"
     CDS             15454..15507
                     /locus_tag="ACHKUC_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618200.1"
                     /translation="MNLLVRCAGKIPALALT"
     gene            complement(15500..15649)
                     /gene="hokB"
                     /locus_tag="ACHKUC_001943"
     CDS             complement(15500..15649)
                     /gene="hokB"
                     /locus_tag="ACHKUC_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO6618201.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            16039..17678
                     /gene="trg"
                     /locus_tag="ACHKUC_001944"
                     /pseudo
     CDS             16039..17678
                     /gene="trg"
                     /locus_tag="ACHKUC_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(17716..18639)
                     /gene="ydcI"
                     /locus_tag="ACHKUC_001945"
     CDS             complement(17716..18639)
                     /gene="ydcI"
                     /locus_tag="ACHKUC_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6618202.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            18856..20199
                     /gene="ydcJ"
                     /locus_tag="ACHKUC_001946"
     CDS             18856..20199
                     /gene="ydcJ"
                     /locus_tag="ACHKUC_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6618203.1"
                     /translation="MANSITADEIREQFSQAMSVMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRSAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGGPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         4978 a         4743 c         5361 g         5209 t
ORIGIN      
        1 atttttctta atccgtttta ttacagggca gggtacgatg agcagcaaca catttactct
       61 cggtacaaaa tccgttaacc gtcttggtta tggcgcgatg caactggctg gtcctggggt
      121 ttttggcccg cccagagatc gccacgtcgc tataaccgtg ctgcgcgagg cgctggcatt
      181 gggcgtcaat catattgata ccagcgactt ttatggtccg cacgtcacca atcagattat
      241 ccgcgaagcg ctttatccgt actctgacga cctgacaatt gtcactaaaa ttggtgcgcg
      301 gcgtggagag gacgcttcct ggttacccgc attttctccg gcagagctgc aaaaagcggt
      361 acacgataat ctacgtaatc tcggcctgga cgtactggat gtggttaacc tgcgcgttat
      421 gatgggggat ggtcatggcc cgacggaagg atcgattgag gccagcctga cagtgctggc
      481 ggagatgcaa caacaaggcc tggtaaaaca tattggcctg agtaacgtca caccgacgca
      541 ggttgcagag gcgcgaaaga ttgccgaaat cgtctgcgtg cagaacgaat acaacattgc
      601 ccaccgtgct gatgacgcaa tgattgatgc tttggctcgt gatggcattg cctacgtgcc
      661 gttcttcccg ctcgggggct ttacgccgct gcaatcctcc acgctttccg atgttgctgc
      721 gagccttaat gcgacaccaa tgcaggtggc gctggcgtgg ctgttacagc gttcaccgaa
      781 tattttgctg attcctggaa catcttctgt tgcgcattta cgggagaata tggctgctga
      841 aaaattgcat ctttctgaga aagtgttgtc tacgttggat ggtatttcgc gagaataacg
      901 aatatacaaa agggaaagat gcatttccct tttgtttctt ttttaatggc atggagtgca
      961 tatgtttaag gccaggattt gcggctggat aatcagggaa aaggcgatgg attcattgat
     1021 tcgtacagtg atagatggta tctcactgat atccagacgg taatcaaaca gatgtgtagg
     1081 gcattcatcc accacatctt gatatattcc gaatttacgt tgatgctgcg cgttgtgata
     1141 tctccgccgt cggaattgat gcaccataga gcaaactgaa tatcacggaa tgataaatga
     1201 cgctctacca gataaaaccg ctctttcagc cgctgttaag accgacgatg ttttggcttt
     1261 ataagcacca tgttacagcg aatcacatca ctcttgctgc actggcgctt tctcttatta
     1321 caggattgct gttgatgttg gtcgcacaac ccatcctctt tttgctattg cccatcgtgc
     1381 tttttgtccg tatggcactc aatgcgctgg atggcatgat ggcgcgtgag tgcaatcagc
     1441 aaacacgtct tggggcgatt ttgaatgaaa ctggcgatgt tatttccgat attgcgcttt
     1501 atttaccgtt tttattttta ccggtaagta acgcatcact cgtaatactc atgttatttt
     1561 gcaccatatt gactgagttt tgcggtttac tcgcgcagac gattaacggt attcgcagtt
     1621 atgccggtcc atttggcaaa agcgatcgcg cgttaatatt tggattgtgg gggctggccg
     1681 ttgccattta tccacagtgg atgcaatgga ataatctttt atggagcatt gcttcaattc
     1741 tgcttctctg gactgcgatt aatcgttgtc ggagtgtgct ttttatgagt gctgaaagat
     1801 aatgctggaa aaatctctgg caacactttt cgcgttgtta attttagcaa cgctgataaa
     1861 ccgttttctg gtatggcggt taccggagag aaaagggggt gaggttacat tacgtattcg
     1921 tacctggtgg ggcatcgtca tttgtttttc actggtgatt tcaggtccac gctggatgac
     1981 gttgacgttt tttgcgctga taagttttct ggcattgaaa gaatattgta cgcttatatc
     2041 tgtacatttt ccgcgttggt tatattgggg tattcctctt aactatttgc ttatcggttt
     2101 taattgcttt gagctgtttc tgttatttat acctttggct gggtttctga tattagccac
     2161 ctggcgagtt ttagtgggtg acccctccgg ttttctgcat accgtgagcg ccattttttg
     2221 tggctggata atgaccgttt tcgccttgag tcatgccgcc tggttattaa tgttgccaac
     2281 cacaaatatc cagggcgggg cgttactggt gttatttctt ttggcattaa ccgagtcaaa
     2341 cgatattgca cagtatttat ggggaaaatc ctgcggcaga agaaaagtgg tccctaaagt
     2401 cagcccggga aaaacattgg aaggtctgat gggcggcgta atcaccacca tgattgcgtc
     2461 actgattatt ggaccgttgc ttacaccgct agatacatta caggcattat tagcgggttt
     2521 gttaatcggt attagtggtt tttgcggcga tgtagtgatg tcagccatca aacgagatat
     2581 tggtgttaaa gatagtggaa aactattacc aggacatggc ggactccttg acaggattga
     2641 ctcattaatt ttcaccgctc cggtattttt ttattttatt cgctactgct gttactgaag
     2701 gaaaatagag aaaatggaaa attcacgcat ccctggggag cattttttta ccaccagtga
     2761 taatacagcg ttgttttatc ggcactggcc cactttactg ccaggggcga aaaaggtcat
     2821 cgtcttattt catcgcggtc atgaacattc tggtcgtcta caacatatcg ttgatgaact
     2881 ggcgatgcca gatactgctt tttatgcatg ggatgcccga gggcatggac aaacttcggg
     2941 gccgcgtggt tatagtccgt ctcttgcgcg ttcagtgcag gatgttgatg aatttgtccg
     3001 ttttgctgcc agcgacagcc aggtcggact ggaagaggtg gttgtgatcg cgcaaagcgt
     3061 cggcgcagtg atggttgcca catgggttca tgattatgcg cctgctattc gcggactagt
     3121 gctggcatct ccggccttta aggttaaatt gtatgtgccg ctggcacgtc ctgggctggc
     3181 gttatggcat cgtctgcgtg gtctgttttt tattaattcc tatgtgaaag ggcgttatct
     3241 gacccacgat cggcaacggg tggcgagttt caataatgat ccgctgatca cacgggcgat
     3301 tgccgtcaat atcttgctcg atctctataa aacgtctgaa cgtattgtta gcgatgcggc
     3361 ggcgattacg ctccccacgc aacttctgat atcaggcgat gactatgtgg tgcatcgcca
     3421 accgcagatt gatttttatc agcgtttacg tagtcctctg aaagagctgc atctgctgcc
     3481 aggcttttat cacgacacgt tgggtgaaga gaacagggcg caggcatttg aaaaaatgca
     3541 aagctttatc agccgtttat atgctaacaa gtcacaaaaa tttgattatc agcatgaaga
     3601 ccgcactgga ccatcagcgg atcgatggcg gctcctttct ggtggacccg tgccgttatc
     3661 gccggttgat ttggcgtatc gctttatgcg aaaggcgatg aaactgttcg gggcgcactc
     3721 tgcgggcctg catctcggaa tgagtaccgg ctttgattca ggcagttcgc tggattatgt
     3781 ctatcaaaat caaccgcaag gcagtaacgc attcgggcgc tttatcgaca aaatctacct
     3841 gaacagtgtt ggctggcgcg gtattcgcca gcgcaaaacc catttacaaa tgctgattaa
     3901 acaagccgtt gcccatctcc acgccaaagg tttagccgtc cgcgtggttg acattgccgc
     3961 agggcatggg cgctatgtac tggatgcgct ggcaaacgag cccgccataa gcgaaatttt
     4021 gttacgtgat tacagtgagg taaatgttgc acaagggcaa gagatgattg cccaacgggg
     4081 aatgtctggg cgggcgcgtt ttgaacaggg cgatgcgttt aatccggcag aactcagcac
     4141 gttaactccg cggcctacgc tggcgattgt ctctggcctg tatgagcttt ttcccgaaaa
     4201 tgagcaggta aaaaactcac tcgcaggtct tgccaatgcc atcgatccgg gtggcattct
     4261 catctacacc gggcagccgt ggcacccaca actggagctg attgccgggg tgttaaccag
     4321 tcataaagat ggtaaaccgt gggtaatgcg cgtgcgttct caaggggaga tggattcgct
     4381 cgtgcatgat accggatttg ataaatgcac acaacggatt gatgagtggg gcatttttac
     4441 ggtttcgatg gcggtgcgtc gtgataactg aacgccggaa cgtattgcta caaggagctg
     4501 gctggttatt gttgctggcc ccgtttttct tctttacata tggatttctt aatcagttca
     4561 ccgcgactca ggatcttaac aaccatgata tccccagtca ggtattcggt tgggaaacgg
     4621 cgatcccttt tcttccctgg actattgttc cttactggag tctggatctt ttatatggat
     4681 tttcgctgtt catttgtagc tcgacattcg aacagcgccg actggttcac cggcttattc
     4741 tggcaacggt aatggcctgc tgcggttttt ttctctatcc gctgaagttt agttttatcc
     4801 gtcctgaagt gagtggggtg acaggatggc tattttcgca acttgaactg tttgatctgc
     4861 cttataacca gtctccttcg ctgcatatta ttctctgctg gctactttgg cgtcactttc
     4921 gtcagcatct ggctgtgagg tggcgtaaag tctgcggcgg atggttttta ctcatcgcca
     4981 tttcgacgct gacgacctgg cagcatcatt ttattgatgt catcacgggg ctggcggtag
     5041 gtatgttaat tgactggata gtgcccgtcg accgtcgttg gaattatcag aaacctgatc
     5101 aacgtcgaat caaaatagca ctgccatatg tcgtaggcgc gtgctcgtgc attgtgttga
     5161 tggagctaat gataatgctt cagttatggt ggtcagtctg gttatgttgg ccagtattat
     5221 cgctattcat cattggccgt gggtacggtg ggcttggcgc gataacaaca gggaaagata
     5281 gtcaggggaa actcccgccc gccgtttact ggctgacatt gccctggcgt atcgggatgt
     5341 ggctgtctat gcgttggttt tgtcttcgcc tggagccggt gagcaaaatt actgctggtg
     5401 tttatttagg ggcgtttcca cgacatattc cggcacagaa tgcggttctg gacgtcacct
     5461 ttgaattccc tcgcggacga gcgacaaaag atcgactcta tttttgtgta ccgatgttgg
     5521 atctggtggt tccggaagag ggggagctcc gacaggccgt ggcgatgctg gaaacattac
     5581 gcgaagagca aggcagcgtt ctggtccatt gcgcgttggg attatcgcgc agtgcgctgg
     5641 tggtggcggc atggctgtta tgttacggac attgtaaaac cgttgatgaa gcgattagtt
     5701 atattcgagc cagacgctcg cggattgtgc ttaaggaaga gcacaaagcg atgctgaaat
     5761 tatgggaaaa caggtaagcg gattgagatg tggactgaat atctacagtc cacatcaaga
     5821 tcgtgtccgg ttattcagca acaatactgt cgatggctgc tttcgcgtca gactgtgctt
     5881 tcgctgccat ttccgggccg tacgcgatcc cttcggcgaa gacaaatttc acatcggtaa
     5941 tgccgataaa gcccaggaat gtggacagat acggcgtcac cagatccgtt gggccatctt
     6001 tatggatccc accgcggctg gtgataacga tggctttttt accggtgacc aggccttccg
     6061 gacccttctc ggtatagcgg aaagtaacgc ctgcacgtgc aaccaggtca aaatagtttt
     6121 tcaactgggt cgagatattg aagttataca tcggtgccgc aataacgata acgtcgtggg
     6181 ctttcagctc ggcaatcagc tcatcggaaa gcgccagagc ttcctgctga cgcggagtca
     6241 gcggcgcatc gctcggacgc agtgcaccaa ccagttcgcc atccaggacc ggaatcggat
     6301 ttgcagccag gtcacgcacg gtgatttcat cagcggagtg cttttcgcgc cattgttcaa
     6361 caaaataatc ggacaactga ttagactgag agtaccctgc caggatgctg gatttaagaa
     6421 ctaatacctt gctcatggtg tttccttata gatatttgaa tgggcaatgc cccgttgctt
     6481 gttgacactt tattcacaat tctgccacag agatagcgca ataaatcgaa gcctatgttc
     6541 gaatttattg aacaacgcat agaaagccgc gatgtggtac tctatatcta tcatttaaaa
     6601 gaaaattaat caggcagact actgcccact aacgttatga cagaacaaca aaaattgacc
     6661 tttactgcct tgcagcagcg gctggattcg atgatgctgc gtgacagact gcgtttttct
     6721 cgccgtctgc acggcgtgaa gaaggttaaa aatcctgatg cacaacaggc cattttccag
     6781 gagatggcga aagagattga ccatgcggca gggaaagtcc tgctgcgtga agcggcacga
     6841 ccggagatta cttacccgga caatttgccg gttagtcaga aaaagcagga cattctcgaa
     6901 gcgattcgtg accaccaggt ggtgatcgtc gccggggaaa cgggttccgg taaaacgact
     6961 cagctaccga aaatctgtat ggagctgggt cgcgggatta aagggctgat cggccatacc
     7021 cagccgcgtc ggctggcggc gcgaacggtg gcgaaccgta ttgcggaaga gctaaaaacg
     7081 gagccgggcg gttgcatcgg ttacaaagtg cgtttcagcg atcacgttag tgataacacg
     7141 atggtcaagc tgatgaccga cggcatcctg ctggcagaga tccagcaaga ccgcctgctg
     7201 atgcagtacg acaccatcat tattgacgaa gcgcacgaac gcagcctgaa tatcgatttt
     7261 cttctcggct atttgaaaga gttgctgccg cggcgtcctg acctaaaaat tattatcact
     7321 tccgcgacta tcgacccgga acgcttttcg cgccacttta ataatgcgcc gattatcgaa
     7381 gtctccggtc ggacctatcc ggtggaagtg cgctatcgcc cgattgttga agaagccgat
     7441 gacaccgagc gcgaccagtt gcaggcgatt tttgacgccg tagatgaact gagccaggaa
     7501 agccctggcg acattctgat ctttatgagc ggcgagcgag aaattcgcga taccgccgat
     7561 gcgctgaaca agctgaactt acgccatacc gaaatcttgc cgctttatgc acggctttcc
     7621 aacagcgaac agaaccgcgt gttccagtcg cacagcgggc gacgcattgt actggcgacc
     7681 aacgtcgcgg aaacctcgct gaccgtaccg ggtattaaat acgttatcga cccaggtacg
     7741 gcgcgtatca gccgctacag ctatcgcacc aaagtgcagc gtttgccgat tgagccaatt
     7801 tcgcaggctt cagctaatca gcgtaaaggc cgctgcggcc gtgtgtcaga agggatctgt
     7861 attcgtctct attccgaaga cgatttcctc tcgcgcccgg agtttaccga cccagaaatt
     7921 ctgcgtacta acctggcctc ggttattttg cagatgaccg cactgggctt gggcgatatc
     7981 gccgcattcc cgtttgtcga agcaccggat aaacgcaata tccaggatgg cgtgcgtctg
     8041 ctcgaagagc tgggcgcgat caccactgat gaacaggcca gcgcctataa actgacgccg
     8101 ctcggtcgcc agctctcgca gttgcctgtc gacccacgtc tggcgcgtat ggtgctggaa
     8161 gcgcaaaaac atggctgcgt gcgtgaggcg atgattatca cgtccgcgct ctctattcag
     8221 gacccgcgtg agcggccaat ggacaagcag caggcatcgg acgaaaaaca tcgtcgcttc
     8281 cacgacaaag agtctgactt cctcgcgttt gtaaatctgt ggaattatct tggcgagcag
     8341 caaaaggcgc tttcttctaa cgccttccgt cgcctgtgtc gtactgatta tctcaactat
     8401 ctgcgcgtgc gcgaatggca ggatatctac acccagttgc gccaggtggt gaaagaactt
     8461 ggcattccgg ttaacagcga accggcggat tatcgcgaaa ttcacattgc gttgctgacc
     8521 ggtttgcttt cgcatatcgg catgaaagat gccgataaac aagaatatac cggcgcacgt
     8581 aacgcgcgtt tctccatctt ccccggttct ggtttattca aaaagccgcc gaaatgggta
     8641 atggtggcgg aactggtaga aaccagccgc ctgtgggggc gcattgcggc acgcatcgat
     8701 ccagaatggg tggaacctgt cgctcagcat ttaatcaaac gcacctacag cgaaccgcac
     8761 tgggaacggg cgcagggcgc ggtgatggca acggaaaaag tcaccgttta cgggttgccg
     8821 attgttgctg cgcgtaaggt caactacagc cagatcgatc cggcgttatg tcgtgaactc
     8881 tttattcgcc acgcgctggt ggaaggtgac tggcagacgc gtcacgcatt cttccgtgaa
     8941 aacctgaaac tacgggccga agtggaagaa ctggaacaca aaactcgtcg ccgcgatatt
     9001 ctggtggatg acgaaacgtt gtttgagttc tacgaccagc gcatcagcca cgatgtgatt
     9061 tccgcacgcc atttcgacag ttggtggaaa aaagtcagcc gcgaaacgcc tgatttgctc
     9121 aactttgaaa agagcatgtt gatcaaagaa ggggcggaaa aaatcagcaa actggattac
     9181 ccgaacttct ggcatcaggg caatctcaaa ctgcgtttga gctatcagtt tgagcccggc
     9241 gcggatgctg acggtgtgac cgtgcatatc ccgctgccgc tacttaacca ggttgaggag
     9301 agtgggtttg agtggcagat ccccggtctg cgtcgtgagc tgatcatcgc cctgattaaa
     9361 tcgctgccga agccggtacg ccgtaatttt gtgcccgcgc caaactacgc cgaagcgttt
     9421 ttaggccgcg tcacaccgct ggaattgccg ttgctcgaca gccttgaacg agagttacgg
     9481 cggatgactg gcgtcaccgt tgaccgcgaa gactggcact gggatcaggt gcccgatcac
     9541 ctgaaaatca ctttccgcgt ggtggatgac aaaaacaaga agctaaaaga agggcgctca
     9601 ttacaggatc tgaaagacgc cctgaaaggc aaagtgcagg aaacgctgtc tgccgtggcg
     9661 gatgacggta tcgagcagag cgggctgcat atctggagtt ttggtcaact tccggaaagt
     9721 tacgagcaga agcgcggcaa ctacaaagtg aaggcgtggc cggcgctggt ggatgagcgt
     9781 gacagcgtag cgatcaaact gtttgataac ccgctggaac agaagcaggc aatgtggaac
     9841 ggtcttcgcc gtttactgct gctaaatatt ccatcgccga tcaagtactt gcacgaaaag
     9901 ctgccaaaca aagccaagct ggggctgtac tttaatccgt atggcaaagt gctggagctg
     9961 atcgatgact gtatctcctg cggtgtggat caactgatcg acgccaatgg tggcccggtc
    10021 tggacggaag aaggctttgc tgcgctgcat gaaaaagtcc gcgctgaact gaacgacacg
    10081 gtggtggata ttgcgaagca ggtcgagcaa atcctcactg cggtgttcaa tatcaacaaa
    10141 cgtctgaaag ggcgggtgga tatgaccatg gcgctggggc tttctgacat taaagcgcag
    10201 atgggcgggc tggtgtatcg cggttttgtc actggcaacg gttttaaacg gttgggcgac
    10261 acgttacggt atttgcaggc gattgaaaaa cgactggaaa aactggcgat tgatccgcat
    10321 cgtgaccgtg ctcagatgct gaaagtcgaa aacgtccagc aggcatggca gcaatggatc
    10381 aacaaactgc cgcctgcacg tcgtgaggat gaagacgtga aagaggtccg ctggatgata
    10441 gaagagctgc gcgttagtta cttcgctcaa caacttggta cgccttatcc gatttcggat
    10501 aagcgtattt tgcaggcgat ggagcagatt agcggttaac actgctattt gcctgataaa
    10561 tctaaaaccc ggtaagcatt tagcgccggg ttttttatta attctaaaaa cgataaggga
    10621 aaaaattcag gagttcagaa aaatacaatt ctctgctgca agatgaataa tgtttatcta
    10681 cagcatttcc ttaaaaaata tgtcaggctt gcggagtggc agttaaggaa atacgacttc
    10741 ctcctttcag agtgctccgc ttctcactat tatctcacgc agtattctta agggaacgat
    10801 aaggaggaac catgaatatt accccgtttc cgacgctttc gccggcaacc atagatgcca
    10861 taaatgttat cggacagtgg ctggcgcagg atgatttctc cggtgaggtg ccgtatcagg
    10921 ccgattgcgt gatccttgca ggcaatgcgg ttatgccgac tatcgatgcg gcatgtaaga
    10981 ttgcccgcga tcagcaaatt cctttactga ttagtggtgg tatcggtcac tcgacaactt
    11041 ttttgtatag cgccatcgca cagcatccac actacaacac tatccgcacc actggcagag
    11101 ctgaagctac catcctggcg gatatcgctc atcagttctg gcatattccg catgaaaaaa
    11161 tctggattga agaccagtca acaaactgcg gtgaaaacgc acgctttagc atcgcgctat
    11221 tgaatcaggc cgtagaacga gttcatacgg ctatcgttgt tcaggacccc accatgcagc
    11281 ggcgcacgat ggcgacgttc cgccgtataa ccggggataa tcccgatgca ccacgctggt
    11341 taagttatcc cggattcgtt cctcagttag gaaataacgc agacagtgta atctttgtta
    11401 atccgttgca aggattatgg ccggttgagc gttatctatc gctactcact ggcgagctgc
    11461 cgcgtttacg cgatgatagc gatggctacg gcccccgcgg gcgagatttt atcgttcacg
    11521 ttgattttcc ggcagaagtc atccaggcat ggcaaacgct gaaacacgat gcggtgctca
    11581 tcgaggcgat ggaaagtcgc tcgttacgtt aaaaattgcc cgttcgtgaa ccaattgttt
    11641 gcaaacgggc atgacttctg acttttattt ctgcctttaa ttccttttac acttgttttt
    11701 atgaagccct tcacagaatt gtcctttcac gatgccgtct ctctgataat tgatgttaat
    11761 taacaataaa tttaccaaaa acaaacatgc aaatcacagg agtcgcccat gtcagtaccc
    11821 gttcaacatc ctatgtatat cgatggacag tttgttacct ggcgtggaga cgcatggatt
    11881 gatgtggtaa accctgctac agaggttgtc atttcccgca ttcccgatgg tcaggccgag
    11941 gatgcccgta aggcaatcga tgcagcagaa cgtgcacaac cagaatggga agcgttgcct
    12001 gctattgaac gcgccagttg gttgcgcaaa atctccgccg ggatccggga acgtgccagt
    12061 gaaatcagtg cgctgattgt cgaagaaggg ggcaagatcc agcagttggc tgaagtcgaa
    12121 gtggctttta ctgcggacta tatcgattat atggcggagt gggcacggcg ttacgagggc
    12181 gagattattc aaagcgatcg tccaggagaa aatatccttc tgtttaaacg tgcgcttggc
    12241 gtgactaccg gcattctgcc gtggaacttt ccgttcttcc tcattgcccg caaaatggct
    12301 cccgctcttt tgaccggtaa taccatcgtc attaaaccca gtgaatttac gccaaacaat
    12361 gcgattgcat tcgccaaaat cgttgatgaa ataggccttc cgcgcggcgt gtttaacctt
    12421 gtactcgggc gtggtgaaac cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg
    12481 gtcagtatga caggcagcgt ctctgcaggt gaaaagatta tggcgactgc ggcgaaaaac
    12541 atcaccaaag tgtgcctgga attggggggt aaagcaccgg ctatcgtaat ggacgatgcc
    12601 gatcttgaac tggcagtcaa agccatcgtt gattcacgcg tcattaatag tgggcaagtg
    12661 tgtaactgtg cagaacgtgt ttatgtacag aaaagcattt atgatcagtt cgtcaatcgg
    12721 ctgggggaag cgatgcaggc ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg
    12781 atgggaccgt tgattaacgc cgcggcgctg gaaagggttg agcaaaaagt ggcgcgcgca
    12841 gtagaagaag gggcgagagt ggcgttgggt ggcaaagcgg tagaggggaa aggatattat
    12901 tatccgccga cattgctgct ggatgttcgc caggaaatgt cgattatgca tgaggaaacc
    12961 tttggcccgg ttctgccggt ggtcgcattt gacacgctgg aagaggctat ctcaatggct
    13021 aatgacagtg attacggcct gacctcatca atctataccc aaaatctgaa cgtcgcgatg
    13081 aaagccatta aagggctgaa gtttggtgaa acttacatca accgtgaaaa cttcgaagct
    13141 atgcaaggct tccacgctgg atggcgtaaa tccggtattg gcggcgcaga tggtaaacat
    13201 ggcttgcatg aatatctgca gacccaggtg gtttatttac agtcgtaatg agtgaaagag
    13261 gcggaggttt tttcctccgc ctgtgcgcgt cagagtttag cgaatttttc gagggtgcga
    13321 ataagctgcg tgacgaagcc atattcgtta tcgtaccagg cgaccgtttt caccagttgt
    13381 aaatcgccca cggcggtaat ttccgtttgc gtggcatcaa acaccgaacc gaaatggctg
    13441 ccaatgatat cggaagagac tatttcttca tcagtataac caaatgactc gttattgttt
    13501 gtcgcctttt taagcgcgtt gttcacctct tcggcagtca ctttctttcc gagaatcgac
    13561 accagttcag tgaccgaacc tgttttcacc ggcacgcgtt gcgcatgacc tttcagtttg
    13621 ccgctcagtt ccgggatcac cagaccaatg gcttttgccg cccccgtagt gtggggaatg
    13681 atattttctg ccgctgcgcg tgaagcacgt aaatctttac cacgtgggcc atccaccagt
    13741 gactgggtgc cggtataggc atgaatggtt gtcatcgtgc cgacttctat cccgaaactg
    13801 tcatgcaagg ctttggccat cggcgcaagg cagttagtgg tgcatgacgc cacggaaaca
    13861 atggtgtcgt tgccatccag agtgtcgtca ttgacgttat aaacgatggt tttcatttca
    13921 ccggcagggg cggaaatcaa caccttcttc gcgccagcat caagatgcgc ctgcgatttc
    13981 tcggcggagg tataaaagcc tgtacattcg acaatgattt ctgcgccttt cgctttccac
    14041 ggaatatttt tagcctcttt ttcggcgtaa accgcgatac ttttcccatc aacgataagt
    14101 gaatcttccg tataatcaac gctccagggg aacggtccgt agtttgaatc atgtttcagc
    14161 aggtaggcga gaatttttgg ggaagtgaga tcattaatag cgacaacgtc tatgttgctt
    14221 ttgacttcaa gtaatcgacg caacaccagt cgaccgatac gaccaaaacc attaatacca
    14281 actttactca tggtcttctc ctgtcaggaa cgttcggatg aaaattgatc ctttccaggc
    14341 ttagaccagg atggcgggat gggcaatcta cattcttgca gtgaaacgta acgtactgaa
    14401 aacgggtgaa caatatttaa tgaaatttta agaaaagccc gttatgttaa cggcaaatta
    14461 tgttaaagca ggaaatgtta tgggaaataa atactcaagg ttacaaatca gcattcactg
    14521 gctggtcttt ttgctggtta tcgtagcgta ttgcgcaatg gagtttcgtg gtttcttccc
    14581 acgtagcgat cggcccctca tcaacatgat tcatgtttcc tgtggcatct caattctcgt
    14641 actgatggtg gttcgtcttt tgttaaggct gaaatgccca accccgccga ttatacctaa
    14701 gccaaaaccg atgatgacgg gactggcgca tttgggccat ttggtgattt atctcctgtt
    14761 tattgcgctg ccagtgattg gtttggtgat gatgtataac cggggcaacc cgtggtttgc
    14821 gtttggtttg acgatgcctt acgcttcaga ggccaatttc gagcgggttg atagcttaaa
    14881 gtcgtggcat gaaacgctgg cgaatctggg atattttgtc atcgggttgc acgctgcggc
    14941 tgcactggca caccactatt tctggaagga caacacactt ctacgcatga tgccgcgtaa
    15001 acgttcctga aggatattta aagaaaacgc ctgtactaaa accgacccgt ggtacaggcg
    15061 aagaatacgg gcctacatcg gaagcgccta ttatatttat ttgtatgaca aataaaacca
    15121 taattcttgt ctatacgtcc atctggctta tctttaatca attcacccga tctttgatct
    15181 catcaacggt atcaaaataa accagcgtat aacgttcaaa tcgaacataa gattcgataa
    15241 atcatggagg ttatatgaaa aaactggcac ttatcctggt tatgggaacg cttgtttcct
    15301 tttatgccga tgccgggcgc aaaccctgtt ctggttcgaa aggggggatc tcacactgta
    15361 cggcaggcgg caaatttgtc tgtaatgatg gttctattag tgcatcgaaa aaaacatgca
    15421 ctaactgaag tgtaaaaggg gtgccatgag aaaatgaatc tgctagtcag atgcgcgggg
    15481 aaaatccccg cgcttgccct tacctagacg tgcaggccat gagcgcagca acctccttat
    15541 caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt caggagtgtg aatgtcagaa
    15601 tcgtaatgca gataatgagc agacacacca ccagagggtt gcgcttcata gcctttctcc
    15661 ttgccggatg gcgggtaaga ggctaacatc tgaattgctt ggttcattcg ttggcctcgg
    15721 ttgatagaaa tatcggtcgg ggccttcgtc tttctgattc ccggttagcc tgaaaacaga
    15781 aagtctcagg cacccgcagg catcctatga ggtttcctta gggacgaaaa taatcacttg
    15841 acgaaattgc gtgctgtttt ccagaatttt tcgtcattcg gtttagccgt tcgttactct
    15901 cttcattcca atggcattat ttttctctgc cataattgtt gtaaaaatgt gacgcaaaga
    15961 ggtttttggt cataagtaat taccgtcaag tgccgatgac tttctatcag gagtaaacct
    16021 ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt tttgcatcac
    16081 atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct attcgcatta
    16141 agttctgctc tggctggcta tttcctctgg caggccgatc gcgatcagcg tgatgttact
    16201 gcggagattg agattcggac cggttagcga acagttcaga ttttttgcgt tcagcccgga
    16261 tcaatatgat tcaggccggg gcagcgagtc gtattgcgga aatggaagca atgaagcgaa
    16321 atattgcgca agccgaatcg gagattaaac agtcgcagca aggttatcgt gcttatcaga
    16381 atcgatcggt gaaaacacct gctgatgaag ccctcgacac tgaattaaat caacgcttcc
    16441 aggcttatat cacgggtatg caacccatgc tgaaatatgc caaaaatggc atgtttgagg
    16501 cgattatcaa tcatgaaagt gagcagatcc gaccgctgga taatgcttat accgatattt
    16561 tgaacaaagc cgttaagata cgtagcacca gagccaacca actggcggaa caggcccatc
    16621 agcgcacccg cctgggtggg atgttcatga ttggcgcgtt tgtgcttgcc ctggtcatga
    16681 cgctgataac atttatggtg ctacgtcgga tcgtcattcg tccactgcaa catgccgcac
    16741 aacggattga aaaaattgcc agtggcgatc tgacgatgaa tgatgaaccg gcgggtcgta
    16801 atgaaatcgg tcgcttaagt cgtcatttac agcaaatgca gcattcactg gggatgacag
    16861 tagggactgt tcgacagggt gcggaagaga tttatcgtgg caccagcgaa atttcagctg
    16921 gcaatgcgga cctgtcatct cgcaccgaag aacaagcggc ggctatcgaa caaactgccg
    16981 ccagcatgga gcaactcact gcgacggtga aacagaatgc ggataacgcg catcatgcca
    17041 gcaaactggc gcaagaggct tctattaaag ccagcgatgg cgggcagatg gtttccggtg
    17101 tagtaaaaac gatgggcgct atctccacga gttcgaagaa aatttctgag atcaccgccg
    17161 tcatcaacag tattgctttc cagacgaata ttctggcact gaatgctgcc gttgaagccg
    17221 cgcgagcggg tgagcaaggc cctggatttg ccgttgtcgc cagcgaagta cggacactcg
    17281 caagccgcag cgctcaggcg gcgaaagaga ttgaaggctt gatcagtgaa tcagtcaggt
    17341 taattgacct gggagcggat gaggtggcca cggccgggaa aaccatgagc actattgttg
    17401 atgccgtcgc gagtgtcaca catatcatgc aggaaatcgc cgccgcctcg gatgaacaaa
    17461 gcagaggcat aacacaggtt agccaggcga tttctgaaat ggataaggtg acgcaacaga
    17521 atgcttctct ggtagaagag gcctctgcgg cggcggtgtc ccttgaagaa caggcggcac
    17581 gattaactga ggcggtggac gtattccgtc tgaacaaaca ttctgtgtcg gcagaacctc
    17641 gcggagctgg tgaaccagtt agtttcgcta cagtgtgaaa atgttaaagg agggatcgac
    17701 agatcccttc acctttcaga acggcattga ttttcgaata gcgttaatca tcaactggca
    17761 accagaagag aacgtcgcat ctacgcgggt cagtattcca atcggttcgc ctgcaccatg
    17821 tcccggaaca ggcagggcta ccagcgtggc atgacgcagg tcgtctttta cagcgccaga
    17881 agggacaaac cacacgtaat cgtattcaac cgtaagttga cgagatagcg aagcagacag
    17941 cgtttcgata caacccgaag gaattttaca tccctggctc tgcactaatg catctgaatg
    18001 ctggcgtggc gcagtgcctt ctggtgatac aacgaccggc cattccagca cccggcttag
    18061 cgttacgttc tcctgaagta gcgggtgatt ggggcggaca accagcttca gcgattcaag
    18121 aaacagcagt tcataattaa gcccgttcat cagttcagga tctgacatcc gaccaatgcc
    18181 gatatcgatt tccccggttt ttaaacccgc cagaatcata gggttactca ttgtcgcaac
    18241 ttgcaaggtc gtctcttttt gttgctgatg aaactgacct ataaccgaag gtaatatccc
    18301 tagtgccgca gtaggtagcg caccaaccct gacgacatca ttattaagac cttctttacg
    18361 atgaagcgac tgtccggcag tgttgatggc gtcaagaact ctgactgcat gcgttaaaaa
    18421 ttgttcgccg ggtaaggtaa gttgcgcccc ctgacgacca cgctcaaaca agcgagcgcc
    18481 cgtcagctgc tccagttcat tcaatgtctt agagagcgca ggttgactca aattaagggt
    18541 ttcagccgcg cgccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg
    18601 caaacggatg cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc
    18661 ggtgtcgctg acaagtgaag ttgttttatt atgataactt gattgcaaaa tattattaac
    18721 aattaaagca actatgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac
    18781 agtttgtaaa ttcttccagc cggagtgaat gcggttacct acactccaga ttactgacca
    18841 ctggaggcag acactatggc gaacagcatc acggcggatg agattcggga acagttttcg
    18901 caggcaatgt cagtcatgta ccagcaagaa gttccgcagt acggcacgct gctggaactg
    18961 gtagctgatg taaatctggc ggtgctggaa aacaatcctc aactgcacga aaaaatggta
    19021 aatgcagacg agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact
    19081 gcacaagagc ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc
    19141 tattacgatc tctcgcaggc aggggtgccg gtacattcga cagcatttcg gcccattgat
    19201 gatgcttctc tggcgcgtaa tcccttccgc gtttttacct cgttactccg tcttgagctt
    19261 atcgagaacg aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc
    19321 accccacgtt gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca
    19381 caggcacagg agtttgtgca ggaagccctg gaaacgtttc gctggcacca gtcagcaacg
    19441 gtagatgaag aaacctatcg cgccttgcac aacgaacatc ggttgattgc tgatgtggtc
    19501 tgttttcctg gatgccatat caaccatctg acgccacgta cgctggatat tgaccgggtg
    19561 cagtcgatga tgcctgaatg cggaattgaa cccaaagttc tgatcgaagg gccgccgcgc
    19621 cgcgaggtgc cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg
    19681 tttgcggggc agaaacaggg cacacatacc gcgcgctttg gtgaaattga gcagcgtggt
    19741 gtggcattaa cgccgaaagg gcgacaactg tatgatgatc ttctgcggag cgctggaacc
    19801 gggcaggata atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac
    19861 agtgagtttt taatgcgtca gcaagggctg ggatggttcc ggtaccgtct gacgccttcg
    19921 ggtgaggcgc atcgtcaggc gattcatccc ggagacgatc cacagccctt aatagaacgt
    19981 ggttgggtag tggcgcaacc cattacctat gaagatttct tgcccgttag cgcggcgggg
    20041 atcttccagt caaatctggg taatgaaacg caggcatgca atcacggtga tgccagtcgc
    20101 gaagcatttg agcaggcgtt ggggggtccg gttttggatg agttccagct ttatcaggaa
    20161 gcggaagaac gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc
    20221 aaaaatttgc ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc
    20281 aatttatgca t
//
LOCUS       DAZFRG010000068        20291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFRG010000068 DAZFRG010000000
VERSION     DAZFRG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.068"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..953
                     /gene="yiaJ"
                     /locus_tag="ACHKUC_001947"
     CDS             105..953
                     /gene="yiaJ"
                     /locus_tag="ACHKUC_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO6618204.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            1055..1528
                     /gene="ysaA"
                     /locus_tag="ACHKUC_001948"
     CDS             1055..1528
                     /gene="ysaA"
                     /locus_tag="ACHKUC_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6618205.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(1680..2933)
                     /gene="avtA"
                     /locus_tag="ACHKUC_001949"
     CDS             complement(1680..2933)
                     /gene="avtA"
                     /locus_tag="ACHKUC_001949"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO6618206.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3111..5141)
                     /gene="malS"
                     /locus_tag="ACHKUC_001950"
     CDS             complement(3111..5141)
                     /gene="malS"
                     /locus_tag="ACHKUC_001950"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6618207.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISLQNTVEPVASLVRECPKWDGLPLTLDVSTTFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDIVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            5461..6252
                     /gene="bax"
                     /locus_tag="ACHKUC_001951"
     CDS             5461..6252
                     /gene="bax"
                     /locus_tag="ACHKUC_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO6618208.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMY"
     gene            complement(6393..7571)
                     /gene="xylR"
                     /locus_tag="ACHKUC_001952"
     CDS             complement(6393..7571)
                     /gene="xylR"
                     /locus_tag="ACHKUC_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO6618209.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYVFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(7649..8830)
                     /gene="xylH"
                     /locus_tag="ACHKUC_001953"
     CDS             complement(7649..8830)
                     /gene="xylH"
                     /locus_tag="ACHKUC_001953"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO6618210.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(8808..10349)
                     /gene="xylG"
                     /locus_tag="ACHKUC_001954"
     CDS             complement(8808..10349)
                     /gene="xylG"
                     /locus_tag="ACHKUC_001954"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO6618211.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(10427..11419)
                     /gene="xylF"
                     /locus_tag="ACHKUC_001955"
     CDS             complement(10427..11419)
                     /gene="xylF"
                     /locus_tag="ACHKUC_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6618212.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            11785..13107
                     /gene="xylA"
                     /locus_tag="ACHKUC_001956"
     CDS             11785..13107
                     /gene="xylA"
                     /locus_tag="ACHKUC_001956"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO6618213.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            13179..14633
                     /gene="xylB"
                     /locus_tag="ACHKUC_001957"
     CDS             13179..14633
                     /gene="xylB"
                     /locus_tag="ACHKUC_001957"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO6618214.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGVLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFHRLYQQLLPLMA"
     gene            14802..15143
                     /gene="yiaB"
                     /locus_tag="ACHKUC_001958"
     CDS             14802..15143
                     /gene="yiaB"
                     /locus_tag="ACHKUC_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO6618215.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            15189..15626
                     /gene="yiaA"
                     /locus_tag="ACHKUC_001959"
     CDS             15189..15626
                     /gene="yiaA"
                     /locus_tag="ACHKUC_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO6618216.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(15667..16662)
                     /gene="wecH"
                     /locus_tag="ACHKUC_001960"
     CDS             complement(15667..16662)
                     /gene="wecH"
                     /locus_tag="ACHKUC_001960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO6618217.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            16837..17136
                     /gene="ysaB"
                     /locus_tag="ACHKUC_001961"
     CDS             16837..17136
                     /gene="ysaB"
                     /locus_tag="ACHKUC_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO6618218.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            17231..18142
                     /gene="glyQ"
                     /locus_tag="ACHKUC_001962"
     CDS             17231..18142
                     /gene="glyQ"
                     /locus_tag="ACHKUC_001962"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO6618219.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            18152..20221
                     /gene="glyS"
                     /locus_tag="ACHKUC_001963"
     CDS             18152..20221
                     /gene="glyS"
                     /locus_tag="ACHKUC_001963"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO6618220.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         5071 a         4962 c         5150 g         5108 t
ORIGIN      
        1 tctgattgat gaatttcaaa aaacttaaaa atcaagtggt tataaattat ctgtcgagat
       61 cgtgaactac ggcacacttt gcgctaccat caggacgcga caaaatgggg aaagaagtga
      121 tgggaaaaaa agagaacgag atggcgcagg aaaaagagcg cccagccgga agccagagtc
      181 tgtttcgtgg gttgatgctg attgagattt tgagcaacta tccaaacggt tgtccgttgg
      241 cgcatctttc ggagctggct ggtttaaata agagtaccgt ccatcgctta ttgcagggat
      301 tacagtcctg cggctatgtg accaccgcgc ccgccgcagg gagttatcgc ctgaccacca
      361 aatttattgc cgtcgggcag aaggcgctat cttcgctgaa tatcattcat atcgccgctc
      421 cgcatcttga ggctctgaac atcgccactg gtgaaactat taacttctcc agccgcgaag
      481 acgatcacgc tattttgatt tataagctgg aacccacaac cgggatgctg cgaacccgtg
      541 cctatatagg ccagcatatg ccgctctact gttccgcaat gggcaagatc tacatggcgt
      601 ttggtcaccc ggactacgtg aagtcatact gggaaagcca tcagcatgag atccagccgt
      661 taacccgcaa taccattacc gagctgcccg cgatgttcga cgaactggcg cacattcgtg
      721 aaagtggagc ggcgatggac agagaagaaa acgaactcgg cgtctcctgt attgctgttc
      781 cggtgtttga tattcatggg cgggtgccgt acgccgtgtc gatttcgctt tcgacatcac
      841 gtctgaaaca ggtgggagag aaaaatctcc tgaaaccact gcgtgaaacc gcgcaggcta
      901 tttctaatga actgggattt actgtcaggg acgaccaggg cgcgattaca taaccctttg
      961 gacaagtgcc aaaactttaa catttccttc gttggatcaa agcagtaggg acgcgctctc
     1021 tggcactctg ctgttttagt gcaaaggagt gatcatgaac cggtttatta ttgcggatgc
     1081 gacgaaatgt atcggttgcc gtacctgtga agtggcttgc gcagtgtcgc atcaggagaa
     1141 tcaggattgc gctgcgttgt caccagacga gtttatttcc cgtattcgtg tcattaaaga
     1201 tcatacttac accacggcag tagcctgtca tcagtgtgaa gatgcaccgt gcgcaaatgt
     1261 gtgccctgtt gacgcgataa gccgcgaaca tgggcatatt ttcgttgaac aatcacgttg
     1321 cattggctgt aaaagctgta tgctggcttg cccgtttggt gcgatggagg tcgtttcttc
     1381 gcgcaaaaag gcgagggcga ttaagtgcga tctgtgctgg catcgggcga cggggccggc
     1441 ctgtgttgaa gcctgcccga caaaggcgtt gcagtgcatg gatgtcgaga aagtgcagcg
     1501 gcatcggcta cggcagcagc ctgtttgaaa cgtttttccg gcgtttctca acgcagggat
     1561 agtgcgcttt gtttatgccg gatgcggcta aaacgcctta tccggcctac aaattctacc
     1621 aaattcaata tattgcagaa atcatgtagg cctgataagc gtagcgcatc aggcaatttt
     1681 tagtggcttt cagcccaggc tctttctatc tcttccgcca gaatcttcac acccgcctca
     1741 attttctccg gctctggtac gtagttcatg cgcatacatt gatgcgtatg cggccacggt
     1801 ttatccagtc ccgggaagaa gttgtgcccc ggcaccatca gcacgccgcg tgctttcagg
     1861 cgctggtaga gctgctcggt cgtaatgggc aaatccttaa accatagcca gaggaaaatg
     1921 gctccttccg gtttatgaat caggcagcga tcttccggta aatagcggcg aatgatagcg
     1981 atagtttcct gaacacgctg gtagtaaaac ggtttgatga ctgtttcaga caggcgcagc
     2041 agatcgttac gcttaatcat ttcacacatc atcgccggac caataccgcc aggtgccagg
     2101 ctgataatgc cgttcatatt ggtgatggcg gtgatgattt tttcattggc gatgataatg
     2161 ccgcagcggg agccaggtag ccccagcttg gaaagactca tgcacagcac gatattcgga
     2221 ttccatagcg ggcgcgcttc gctgaagatg atacccggga acgggacgcc ataagcgtta
     2281 tcaatcacca gcggaatacc gtgttgattc gccagcgcgt caagcttcag caactcttcg
     2341 tcagtaatca cattgcctgt tggattcgtc ggccgggaga cgcaaatcat cccggtttct
     2401 tcgccaatat gtagatgctc aaaatcgacg tggtatttaa actggccttc cggcagcagt
     2461 tcaatattcg gacgcgcaga gacaaacaga tcttcttcca gtccggcgtc agcgtagcca
     2521 atgtattccg gtgccagcgg gaacagcact tttttgaccc gaccatcggc acggcgtccg
     2581 gcaaacagat taaataagta gaaaaacgcg ctctggctgc cgtttgttag tgcaatattc
     2641 tgtggttcga tatcccaacc caacttctcg cgcagcattc cggcaagcag tgtgagtagc
     2701 tccgttttcc cctgtggacc gtcataattg cacagtgcat cagtcgcttt gccactttcc
     2761 agcatgtcgg tcagtagcgt ctggaagtag tcctgcattt cagggatctg cgccggatta
     2821 ccgccgccga gcataatcgc gccaggcgtg cgtaaaccgt cgttcagatc ttccatcagc
     2881 agcgtaatgc cggagtggcg ggtaaatttg tcaccaaaaa gggagaatgt catagcggga
     2941 tatctgtcga actctgtagc aaggaaggta acaataacgc tacatttgct ggggtgcaaa
     3001 tcggtctgtt gaagagtgag cggtgcttta tcctgcaatg ctgattaggg ctgacatttt
     3061 atcctggttg gcggcttccc gaggtactga cgaacgggaa gccggaaaaa tcactgttgc
     3121 ccagcccaga tgaccagcac tttatcgtcg ccatgctcac gaacaaagcc gtagccctgc
     3181 ttcagcgaaa gtgtcgtttg tttgcccgcg ccaattgcgg gatggcgggc gcggaactgg
     3241 ctgattttct gccagtgcgc gacgctggcg gcagatttac cgctaacatc ctgccagttc
     3301 atatccgaac gcgtaccttg cagcggatca gaaccggtag gaccgaacgg acgcgaggat
     3361 tcatcgccat aaaagatttg taccgcgcct ggcgctaata ataataactc tgctgctttg
     3421 tcgccccctt cacggaacag gcgggtatca tgcgacgaga ggtagctcaa cacgttgaaa
     3481 tcctgcaatt tctccgccat ttgctgccag gtcgtatcca tctgcgccag acaatcgact
     3541 gctttcgccg cctgctcctg ataatcgaaa ttgatcatcg catcgaagcc gtggcgatag
     3601 tagtcacttt gcatcacgcc gtggccccag gcttcaccgg tcatccagaa aggtttgtca
     3661 tctaatgctt tgtcggggtt agcttttttc cattcgcgaa gtgcagcgct ggcttcggtt
     3721 ttcagttgct gccaggcggg caactcaaca tgtttggcgg tatcgacccg aaaaccatca
     3781 atcccatagt cgcggaccca ctgacttaac cagtgggtta agtaatcgcg aggggtaaag
     3841 ccgtcgatga ctttagcgtg ggtatccgtt ttgtttttat agaacactgg cagaccagaa
     3901 gcggtagttg attcggtttt gatatccggc aaaaaggcca gcgacatagt gagatcgtcg
     3961 aatccaggat tgtcgtaatc gccgatatcc gttctgatcc agttttttcc ccaccattta
     4021 tcccagcctg ttttgtcgct gaaattaatg taatcgttaa agctatgcca ggtttgcccg
     4081 gcggcaggtt tccagtcgct ccagcgttca cccagcgttt ttttcagctc gtcaccagaa
     4141 agatataacg cgccaaactg atactcctgc atatccgcca gcgtggcata gccagtgtgg
     4201 ttcatcacga tatcaaagag aatacgaata ccgcgctgat gcgcgctatc aaccagcgtc
     4261 cgtagatcgg cttcactgcc catattggca tcaagattcg tccagtcctg agtgtaataa
     4321 ccatgatagg cataatgcgg gaaatcacct tttgtaccgc cgccgaccca gccgtgaatt
     4381 tgctcaaatg gggcgcttat ccataaagca ttaacgccca actgctggag ataatccagt
     4441 ttgttggtca ggccgcgtaa atcgccgccg tgaaaagtgc caatttccgc cataccgtct
     4501 ttatgacgtc cgtaactctg gtcattactg ggatcgccgt tttcgaaacg atctgtcagc
     4561 acaaagtaaa ccgtggcgtt atgccagtcg aaaggggcgg atgtgtcagt ttctgcccgt
     4621 tccagcagga gtaaaccgtt gctggtggca gcgggttgta acgttatttg accgttcttc
     4681 actatcgcaa tttgctggct gtaataatcc cggacggcgg ctccctccgc aaaagtggtg
     4741 ctgacatcca gtgtgagcgg taatccatcc catttcgggc attcacggac caggcttgct
     4801 accggttcga cggtgttctg gagggaaatc atcaatgttg gcgtaccgga gcgggtgtct
     4861 agttgcagcg tatattcgcc gtccctgaac aatcgccatt gaggtggcgt gttgctacaa
     4921 ggttgcaggg aaagcatctg attgagtttt atcgcatccg caggctgcca gcactgttgg
     4981 tcaaaattta gcattagtgg acgcgtacct ttgggcaact gcgcgtggct gacaaatgtt
     5041 cccgtcccct gttcgctaaa ggcgggaaac cccggagaag tccagctggc ggcaacggcg
     5101 aagccaggaa ggagtgtcag aaaacaggcg gcgagtttca tggcggatga gtccttattg
     5161 ctgcgatttc agacagtttg ccagcgataa gccagacaaa actcatccct gagcggtaag
     5221 tccacaggat gagaagcaag ggtgagcgat cgcgcgcaaa aaacggctga attttgcgat
     5281 aaaccccaca ttttctgcga tttagcgcca atctgaatcg ttaactcgtg acatagtttc
     5341 agatttggac tattccttgg ggtgctcttg acagctattt ttgatatgat ttgagattca
     5401 actctcaaat ttgtaaaaaa tataaggtgt tggaatgatt aaatccgacc aggagacctg
     5461 atgattttga ctcccatacg acgatatggg gcgatgattc ttatgttact cactctggtg
     5521 ttttcgagtg aggtgttagc gaagacgcac acaacaacag cgagtcaaaa gtcccactta
     5581 actaaagcta gtaataaaca ggtaagcagt aaacaagagt attctcgcaa tagtgcaaag
     5641 agtagttcac ttcctgattt gcgaaaatac ccttccggaa caccaaggaa aaaggcgttt
     5701 ctccggactg taatgcctta cattaccagc caaaatgcgg ccattactgc ggaacgtaac
     5761 tggctgattt caaaacagta tcagggccaa tggtcacctg ctgagcgtgc gcgtctgaaa
     5821 gacatcgcca aacgctacaa ggtgaagtgg tctggtaata cgcgaaaaat cccgtggaat
     5881 accttgcttg aacgcgtaga cattatccct accagtatgg tggcgacgat ggctgcagca
     5941 gaaagcggtt ggggaacgtc aaagctggcg cgcaacaaca acaacctgtt cggcatgaaa
     6001 tgcatgaaag gacgttgtac caatgcgccg ggtaaagtga aagggtactc acagtttagt
     6061 tctgtcaaag aatcggtgag cgcctatgtc actaacctca atacgcaccc ggcttactct
     6121 tcgttccgta aatcgcgtgc acaactgcgt aaagcggatc aggaagtgac tgccacagcg
     6181 atgattcaca agctgaaggg ctactcgacc aaagggaaga gttataacaa ctacctgttc
     6241 gcaatgtact aggataacca acggttaatc gcggcgcata tgtaattgca tttcctcgag
     6301 ccttatccga cttgtcaggc ggataaggct tttgctcgta tcaggtggtt gtgctgagtt
     6361 acctgatgcg acgccgataa ttctcatcat cgctacaaca tgacctcgct atttacatcg
     6421 cgatactctt ttggcgttgt gtcatatgct tttttaaaaa cagagtagaa atattgcagc
     6481 gatggataac cgcacatttg cgatatctca ttgatcgaca aggtggttga aatcagcaga
     6541 ctgcgcgctt tctccagctt ctcggcatga atcatggcat ggatggtttc acccacctct
     6601 tctttaaaac gcttctcaag attggagcgt gagattccga ccgcatcgag tacttgatct
     6661 actttaatcc ctttgcaggc atgattacgg atgtaatgca tggcctgaat aaccgcagga
     6721 tcagtcaacg aacggtaatc cgttgaacgt cgttcaatca cgcggactgg cgggaccaaa
     6781 atccgttgca acggcatctc ttctttatcc aataaacgat gcaacagttt tgccgcctga
     6841 tagcccattt gccgtgcgcc ctgagcgacc gaagaaaggg cgacacgcga cagatagcgg
     6901 gtcagttctt cgttatcgat gccaatcacg cataattttt ccggtacggg aatgtgtaga
     6961 tgttcacata cttgcagaat atgccgcgcc cgggcgtcag taacggcaat aatcccggtt
     7021 tgcggtggca gcgtttgtag ccagtctgcc agccgatttt gcgcgtgttg ccagttctct
     7081 ggcgcggttt ctaacccctg ataaaccact ccgcgatact tttcttcggc gacaagctga
     7141 cgaaatacat attcgcgttc agtggcccaa cgtttaccgc ttgattccgg aagaccataa
     7201 aaagcaaagc ggttaacgcc tttctctttt aaatgcaaaa atgcgctttc aaccagcgca
     7261 tagttatcgg tggcaatgta atgaacgggt gggtaacttt cggcaaggtg atacgagcca
     7321 ccaaccccaa caatggggac gtcgacatca gccagcgctt gctcgatttg tttgtcgtcg
     7381 aagtcggcaa tgacgccatc tcctaaccag tccttgattt tatcaatgcg ggcgcggaaa
     7441 tcttcttcaa tgaaaatatc ccattccgat tgtgacgcct gtaaatattc ccctacgcct
     7501 tctactacct gccggtcata ggctttattg gcattgaaca gtaatgtgat gcggtgacgt
     7561 ttagtaaaca tggttctttt cctgctgaat catgcaaaaa ctcaaaaccg gtaatacgtt
     7621 accggctttg agaaaatttt tatcaaaatc aagaacggcg tttggttgcg gagtccatcc
     7681 atactgccag caacagaatc gcacctttaa cgatatactg ccagaaggtc ggtacatcca
     7741 tcatactcat gccgttatcc agcgaagcca tgatgaatgc ccccattacc gctccggcca
     7801 cgctcccgac gccgcctgcc agactggtgc cgccaatcac acatgctgca attgcgtcca
     7861 gttcggcgat atttcccgca gaaggtgaac cagcgccgag tcgagaactg aggattaatc
     7921 cggcgatggc taccattaat ccgttaatag cgaacacggc aagtttggtg cgttcaacgt
     7981 taatcccgga gagacgtgcc gcttccagat tgccgccgat ggcataaatg cgtcgtccaa
     8041 atgccgtccg cgttgccata aacattccgc cgagtaacag caacgtcagc agcagaacag
     8101 gagtgggaac accacggtaa tcattcaata gccagattgc gcctaatacg atgatagcgg
     8161 ttaaagcctg gcgaccgact actgcggtag aggccggaga ctgcaaaccc aaagcctgac
     8221 ggcgcattct tccgcgccat tgccagccaa caaaagccat taagccaagc gcgccaatga
     8281 tgaagccggt gctggcaggt agatagcttt gcccaatttg tgacatcgcg gcgctggtgg
     8341 gggaaacagt cgtgccgttg gtgatgccaa tgagtatgcc acgaaatgcc aacatgcccg
     8401 cgagggtgac aataaatgaa gggactttgc ggtacgcgac ccaccatccg ttccaggctc
     8461 cgagaagcag tcccagaacc aacgtcacaa tgatggtaag tggcaaaggc cagcctagcc
     8521 agacgtcaca aatcgccgcg acgccaccta acagccccat cattgagccg acggaaaggt
     8581 cgatttcagc agaaattatg acgaacacca ttcctaccgc gaggatgccg gtaatcgcgg
     8641 tttggcgtaa caggttggag acgttacggg cgcttaagta ggcaccatcg gtggtccagg
     8701 taaagaacag catgattgcg atgatagctg caatcatcac gaagacctgc aaattcagtg
     8761 atttcagccc ggaaaagcca ccggatgtcg gtacggctaa tttcacttca gacggattgc
     8821 ttttcgacat gatgttcgct cctcaatgcg gcttccatca cctgctcctg agtcaggtta
     8881 tgatttatca ggttggcttt tagtttccct tcatgcatca ccagtacacg atcgctaagg
     8941 ccgagcactt caggtaattc ggaagagatg acaataacgg caataccctg ctggacgagt
     9001 tggttaatta atttgtagat ctcgtatttc gcgccaatat cgatacccct ggtgggttca
     9061 tcaagaatga gaatgcgcgg gttaagtaac agacagcgag cgaggatcgc tttttgctga
     9121 ttgccgccgc tcaaacgtcc aatagcaagg tcgggggacg acgttttaac tttgagttgc
     9181 tggattgatt ccagaataca tttttgctct gccgcgtcat caagctggct aatgccaccg
     9241 gtaaattttt tgagtgcggc gagggtaata tttttaccaa ccgccattac cggaacgatg
     9301 ccgtcgcgct ttctgtcttc gggtaccatc gcaatcccct gggcgatggc ttgctgacag
     9361 ttacgaatat ctacctgttt gccgtcaata taaattttcc cttcccattg tccgggccac
     9421 acaccaaaca ggcactgaat agtctcggta cgtccggcac caacgagtcc ggcgataccc
     9481 agtatttcgc cacgtttcag ggaaaacgag acatcattaa ctcgtttaat atgacgatta
     9541 accggatgcc atgccgtcag atgttcaata cgtaatattt catctccggt ggtatgtggt
     9601 tcattagggt aaagcgcggt taactctcgc ccgaccatca tggtgataat atcgtcttca
     9661 ctcattccag cagcatcacg cgtgccaatg tgctgaccgt cgcgaataac gcaaatcgta
     9721 tcggaaatcg ctttgacttc gttgagtttg tgcgaaatat aaatacaggc gataccgtgc
     9781 tgttgtagat cgcgaataat atccagtaaa accgacgttt cctgctcagt taatgaggct
     9841 gtcggctcat cgagaattaa caaacgcacc tgtttattaa gtgccttggc aatttcaacc
     9901 agttgttgtt gcccaagtcc taaatcgcca acgcgggtat caggtgaaat ggataaactg
     9961 acctgtgcga gcagcttctg acagcgtagc gtcatcaggt cataatccat aatgccatta
    10021 tgggttattt cgttacccag gaagatattt tccagcacgg tcagttcttt caccagcgcc
    10081 aattcctggt gaataatggc gatacctttg cgttcggtat cgcggatgtg actcgcctga
    10141 atctcttctc ccgcaaaaat aatttcgcct tcgtaggaac catggggata aataccacac
    10201 agcactttca tcagcgttga tttacctgac ccattttccc cacagagtga gacgatttcg
    10261 ccagcattca accgcaagct gacgttatca atcgccttca cactgccgaa ggttttggta
    10321 atgttcttca tttcaagtag ataaggcata acgactccac ctaagccaat caattcacgc
    10381 ggcatggaga gaaatcacgc ccccgctccg cgccggggcg taacgcttac agctcgctct
    10441 ctttgtggaa tccgtcttta actaccgtat ctttgatgtt gtttttattc acatcgatcg
    10501 gtgtcagcag gcgggaggga acatctttca ggccattatt cagtgtggta tccgcttttg
    10561 gctcctgacc attgcccaac tcaacggcaa tttctgcggc agtatttgcc aacaatgtaa
    10621 taggtttata caccgtcata gtttgtgtac cggcagcaat acgtttaata cctgcaagat
    10681 ccgcatcctg gccggaaatt gctactttcc ctgataaacc ttgcgcgctt aatgcctgaa
    10741 ttgcccctcc tgcggtggca tcgtttgagg caactacagc atcaattttg ttattattgg
    10801 cggttagcgc gttttccata attttcaatg cgttttccgg taaccagcca tcaacccatt
    10861 ggtcaccaac gactttaatt tttccggaat caacataagg ttttaatact ttcatttgcc
    10921 cggcgcggaa cagcttggcg ttgttatcta ccggcgagcc gcccatcagg aagtaattac
    10981 cttgcggaac aatatcgacc agggcttttg cctgcagttc accgactttt tcattatcga
    11041 aagaaatata aaaatcgata tccgcatcgt taatcatacg gtcgtaagct aatactttaa
    11101 taccttcttg tttggcttct tttacaacgt tacttaatac ctgaccgtta taaggaataa
    11161 tgacaagaac atcgacaccc cggttaatca tgttttcaat ctgcgacatt tgtgtttctt
    11221 cattgccatt tgcagactgt acaaatactt tcgcgccgag agattctgct tttttcacaa
    11281 aaatatctcg atctttttgc cagcgttcaa gacggagatc atcaatcgcc atacctattt
    11341 tgacctcttt ggcgtgtgcc gcaacgttgg taagcagaag tgaggtgcaa agggtgagta
    11401 gaatgttctt tattttcatg gtgtagggcc ttctgtagtt agaggacagc tttaataagt
    11461 aacaatcacc gcgataaacg taaccaattt ttagcaacta aacaggggaa aacaattaca
    11521 gatttttatc tttcgattac gatttttggt ttatttcttg atttatgacc gagatcttac
    11581 ttttgttgcg caattgtact tattgcattt ttctcttcga ggaattaccc agtttcatca
    11641 tcccatttta ttttgcgagc gagcgcacac ttgtgaatta tctcaatagc agtgtgaaat
    11701 aacataattg agcaactgaa agggagtgcc caatattacg acatcatcca tcacccgcgg
    11761 cattacctga ttatggagtt caatatgcaa gcctattttg accagctcga tcgcgttcgt
    11821 tatgaaggct caaaatcctc aaacccgtta gcattccgtc actacaatcc cgacgaactg
    11881 gtgttgggca agcgtatgga agagcacttg cgttttgccg cctgctactg gcacaccttc
    11941 tgctggaacg gggcggatat gtttggtgtg ggggcgttta atcgtccgtg gcagcagcct
    12001 ggtgaggcac tggcgttggc gaagcgtaaa gcagatgtcg catttgagtt tttccacaag
    12061 ttacatgtgc cattttattg cttccacgat gtggatgttt cccctgaggg cgcgtcgtta
    12121 aaagagtaca tcaataattt tgcgcaaatg gttgatgtcc tggcaggcaa gcaagaagag
    12181 agcggcgtga agctgctgtg gggaactgct aactgcttta caaaccctcg ctatggcgcg
    12241 ggtgcggcga cgaacccaga tcctgaagtc ttcagttggg cggcaacgca agttgttaca
    12301 gcgatggaag caacccataa attgggcggt gaaaactatg tcctgtgggg tggtcgtgaa
    12361 ggttacgaaa cgctgttaaa taccgacttg cgccaggagc gtgaacagct gggccgcttt
    12421 atgcagatgg tggttgagca taaacataaa atcggtttcc agggcacgtt gcttatcgaa
    12481 ccgaaaccgc aagaaccgac caaacatcaa tatgattacg atgctgcgac ggtctatggc
    12541 ttcctgaaac agtttggtct ggaaaaagag attaaactga atattgaagc taaccacgcg
    12601 acgctggcag gtcactcttt ccatcatgaa atagccaccg ccattgcgct tggcctgttc
    12661 ggttctgtcg acgccaaccg tggcgatgcg caactgggct gggacaccga ccagttcccg
    12721 aacagtgtgg aagagaatgc gctggtgatg tatgaaattc tcaaagcagg cggtttcacc
    12781 accggtggtc tgaacttcga tgccaaagta cgtcgtcaaa gtactgataa atatgatctg
    12841 ttttacggtc atatcggcgc gatggatacg atggcgttgg cgctgaaaat tgcagcgcgc
    12901 atgattgaag atggcgagct ggataaacgc atcgcgcagc gttattccgg ctggaatagt
    12961 gaattaggcc agcaaatcct gaaaggccaa atgtcactgg cagatttagc caaatatgct
    13021 caggaacata atttgtctcc ggtgcatcag agtggtcgcc aggagcaact ggaaaatctg
    13081 gtaaatcatt atctgttcga caaataacgg ctaactgtgc agtccgttgg cccggttatc
    13141 ggtagcgata ccgggcattt ttttaaggaa cgatcgatat gtatatcggg atagatcttg
    13201 gcacctcggg cgtaaaagtt attttgctca acgagcaggg tgaggtggtt gctgcgcaaa
    13261 cggaaaagct gaccgtttcg cgcccgcatc cactctggtc ggaacaagac ccggaacagt
    13321 ggtggcaggc aactgatcgc gcaatgaaag ctctgggcgt tcagcattct ctgcaagacg
    13381 ttaaagcatt aggtattgcc ggacagatgc acggtgcaac cttactggat gctcagcaac
    13441 gggtgttacg ccctgccatt ttgtggaacg acgggcgctg tgcgcaagag tgcactttgc
    13501 tggaagcgcg agttccgcaa tcgcgggtga ttaccggcaa cctgatgatg cccggattta
    13561 ctgcgcctaa attgctatgg gttcagcggc atgagccgga gatattccgt caaatcgata
    13621 aagtattatt accgaaagat tacttgcgtc tgcgtatgac gggggagttt gccagcgata
    13681 tgtctgacgc agctggcact atgtggctgg atgtcgcaaa gcgtgactgg agcgatctca
    13741 tgctgcaggc ttgccactta tctcgtgacc agatgcccgc attatacgaa ggcagcgaaa
    13801 ttactggtgc tttgttacct gaagttgcga aagcgtgggg tatggcggcg gtgccagtta
    13861 tcgcaggcgg tggcgacaat gcagctggtg cggttggtgt gggaatggtt gatgctaatc
    13921 aggcaatgtt atcgctgggg acgtcggggg tctattttgc tgtcagcgaa ggggtcttaa
    13981 gcaagccaga aagcgccgta catagctttt gccatgcgtt accgcaacgt tggcatttaa
    14041 tgtctgtaat gctgagtgcg gcttcgtgtc tggattgggc cgcgaaatta accggcctga
    14101 gcaatgtccc tgctttaatc accgcagctc aacaggctga tgaaagtgcc gagccagttt
    14161 ggtttctgcc ttatctttcc ggcgagcgta caccacacaa taatccccag gcgaaagggg
    14221 ttttctttgg tttgactcat caacatggcc ccaatgaact ggcgcgagca gtgctggaag
    14281 gcgtgggtta tgcgctggca gatggcatgg atgtcgtgca tgcctgcggc attaaaccgc
    14341 aaagtgttac gttgattggt ggcggggcgc gtagtgagta ctggcgtcaa atgctggcgg
    14401 acatcagcgg tcagcaactg gattaccgca cgggtggcga tgtggggcca gcactgggcg
    14461 cagcaaggct ggcgcagatt gcggcgaatc cagagaaatc gctcattgag ttgttgccgc
    14521 aactaccgtt agaacagtcg catctaccag atgcgcagcg ttatgccgct tatcagccac
    14581 gacgagaaac gttccatcgc ctctatcagc aacttctgcc attaatggcg taaacgttat
    14641 cccctgcctg accgggtggg ggataattca catctatata tctcagtaat taattaatat
    14701 ttagtatgaa tttattctga aaatcatttg ttaatggcat ttttcagttt tgtctttcgt
    14761 tggttattcg taatgtatcg ctggtagata tggagatcgt tatgaaaacc tcaaagactg
    14821 ttgcaaaact attatttgtt gttggggcgc tggtttatct ggttggacta tggatctcat
    14881 gcccattgtt aagtggaaaa ggctattttc ttggcgtgtt aatgacagca acttttggta
    14941 actatgcata tcttcgcgca gaaaaacttg ggcaactgga tgattttttt atccacgtct
    15001 gtcagttggt agcgttaatc actatcggtc tcttgtttat cggtgtttta aacgcaccta
    15061 tcaatgctta tgaaatggtg atctatccca tcgccttttt tgtctgcttg tttggtcaaa
    15121 tgcgtttgtt tcgctcggca tgagcaacat aaagttctta cacattcagg aatgaaagga
    15181 atactgtgat ggacaacaaa atatcaacct attctccggc atttagtatt gtttcatggg
    15241 tagcccttat tggcggtatt gttacctatc tgttaggact atggaatgca gagatgcagt
    15301 taaatgaaaa agggtattat tttgccgtac tggtattagg tttgttttcc gcggcgtctt
    15361 atcaaaagac cgttcgggac aagtatgaag gcatacctac cacccccatt tattatatga
    15421 cttgcctgac ggtctttatt atctctgttg cgttgttgct ggtaggtcta tggaatgcaa
    15481 cgttattact cagcgaaaaa ggcttttatg ggctggcttt cttcttaagc ttgtttggtg
    15541 cagtagcggt gcagaagaat attcgtgatg ccggaataaa cccaccaaaa gaaacacagg
    15601 ttacccagga agaatacagc gaataattca cataagcccg gttagtccaa tgtaaccggg
    15661 cttttactaa ctcactaatc tgtttctgtc gattcgttgt accagcatag aaagtaacaa
    15721 actcgctgcc aacgtcgcgc aaaagatcca aataatatcg agtattggcc agtttttaag
    15781 ctcaatcccc cgggtgcgca gtgcatggat aatcaatgca tggaacccgt atatacctaa
    15841 cgaatggcgg gagattaagc caagtccgca aatggtacgc gtatacagcg tgtttttaac
    15901 cagagtcaat aacgcgattg cgcagataaa aaccatcggc ccacagtaaa gataccaggt
    15961 atcggcaaaa tttccgcgcc actgtaattc atataatgtc ccgcgagaga taataaaaac
    16021 ccccgtcgca aacagtgcgg cgctcaccca cgacagtgct ttatgctgtg tgtccatcat
    16081 ccctatagcg cggcccaaca tgccataaag gatgtagtaa aaagtatcgc cattgatata
    16141 taagttaatt ggcagccatt caaaaccgtc gattttctgc ggcacagtat ttgggttagc
    16201 gatgatacca atcaccacca ttagcaccag caacattttt ccgccgacgt tcttcacctg
    16261 aatcagcggt gaaaccagat aaatcactgc aatcgcgaag aaaaaccata aatggtaaaa
    16321 cactggcttt tgcagcaggt ttttcagcgc taactccaca ttgatggagg taaacagcgc
    16381 aatgtagagc agcgcgattg cgctataaaa aaacaggcat aagccgatac gcaagaaatg
    16441 gcgcgactgg gcgctgcgtt cgccaaaaaa gagatagccg gaaatcatga aaaatagcgg
    16501 cacgctgaca cgagaggcag aattcagaac attggcgata tcccaggtga tggggctaac
    16561 actatgagca ttggtcacat accaggtagt ggtgtgaatc atcaccacca ttaaacacgc
    16621 tatccctcgc aggttatcaa tccagtaaat tttgggctgc atctgtgtct ctgtatctgg
    16681 ttaaaaaaag tctgaccgat aaatcattca gttggcgcac tggaataatc tgagttttat
    16741 cactacagct tatagaggct taaggaaatt cgtaagatat cagccactat accgatataa
    16801 ataataagac tcacctgcaa accagacggt aatttaatga tgatgaacgc tttctttccg
    16861 gcaatggcgc ttattgtgct agtgggttgt tctacaccgc cacccgtgca gaaagcacaa
    16921 cgggtaaagg ttgatcctct gcgttcgttg aatatggaag cgttatgcaa ggatcaggcg
    16981 gcaaaacgtt ataacaccgg cgagcaaaaa atcgacgtca ccgccttcga acagttccag
    17041 ggaagctatg aaatgcgcgg ttataccttc cggaaagagc agtttgtctg ttcttttgac
    17101 gcggatggcc attttttgca tctttccatg cgttaagccc tgctttttcc cgtttcgtac
    17161 tgtatatctt ccatccagcg ggtatactga tcccttcctt taaatccaca cgtatccagc
    17221 acgaaataat atgcaaaagt ttgataccag gaccttccag ggcttgatcc tgaccttaca
    17281 ggattactgg gctcgccagg gctgcaccat tgttcaacca ttggacatgg aagtcggcgc
    17341 gggaacctct cacccaatga cctgtctgcg cgcgctgggg ccagaaccga tggcggctgc
    17401 ttatgttcag ccttctcgtc gcccgaccga tggtcgctac ggcgaaaacc ccaaccgttt
    17461 acagcactac tatcagttcc aggtggtcat taagccatcg ccggacaata ttcaggagct
    17521 gtacctcggt tctctgaaag agctgggcat ggacccgacc atccacgaca tccgtttcgt
    17581 ggaagataac tgggaaaacc cgacgctggg tgcctggggg ctgggctggg aagtgtggct
    17641 aaacggcatg gaagtgacgc agttcactta cttccagcag gttggtggtc tggaatgtaa
    17701 accggttacc ggtgagatca cctacggtct ggaacgtctg gccatgtaca ttcagggcgt
    17761 agacagcgtt tacgacctgg tctggagcga cggcccgctg ggtaaaacca cctacggcga
    17821 cgtgttccat cagaacgaag tggagcagtc cacttacaac ttcgaatacg cggatgtgga
    17881 cttcctgttc acctgcttcg agcagtacga gaaagaagcg cagcaactgc tggcgctgga
    17941 aaatccgctg ccgctgccag cctacgagcg tattctgaaa gccgcccaca gcttcaacct
    18001 gctggatgcg cgtaaagcca tctccgtcac cgagcgtcag cgctatattc tgcgcattcg
    18061 caccctgacc aaagcagtgg cagaagcata ctacgcttcc cgtgaagccc tcggcttccc
    18121 gatgtgcaac aaagataagt aagaggcggc tatgtctgag aaaacttttc tggtggaaat
    18181 tggcactgaa gagctgccac caaaagcact gcgcagcctg gctgagtcct ttgctgcgaa
    18241 ttttactgcg gagctggata acgctggcct cgcacacggc accgttcaat ggtttgctgc
    18301 tccgcgtcgt ctggcgctga aagtcgctaa cctggcggaa gcgcaaccgg atcgtgaaat
    18361 cgaaaaacgc ggcccggcga ttgcccaggc gttcgacgct gaaggcaaac cgagcaaagc
    18421 ggcagaaggt tgggcgcgtg gttgcggtat taccgttgac caggctgagc gtctgactac
    18481 cgataaaggc gaatggctgc tgtatcgcgc ccatgtgaag ggcgaaagca ccgaagcact
    18541 gctgccgaat atggttgcga cttcgctggc gaaactgccg atcccgaaac tgatgcgttg
    18601 gggcgcgtct gacgtacact tcgtgcgtcc ggttcacact gtaaccctgc tgctgggcga
    18661 caaagtcatt ccggctacca ttctgggcat tcagtccgat cgcgtgattc gcggccaccg
    18721 ctttatgggc gagccggaat tcaccatcga taacgccgat cagtatccgg aaattctgcg
    18781 tgagcgcggg aaagtcatcg ccgattacga agaacgtaaa gcgaagatta aagccgatgc
    18841 cgaagaagca gcgcgtaaga ttggcggtaa cgctgactta agcgaaagtc tgctggaaga
    18901 agtggcttcg ctggtggaat ggccggttgt gctgaccgca aaattcgaag agaaattcct
    18961 cgcggtgccg gctgaagcgc tggtttacac catgaaaggt gaccagaaat acttcccggt
    19021 gtatgcgaac gacggcaaac tgctgccgaa ctttatcttc gttgccaaca tcgaatcgaa
    19081 agatccgcag cagattatct ccggtaacga gaaagtcgtt cgtccgcgtc tggcggatgc
    19141 cgagttcttc ttcaacaccg accgtaaaaa acgtctggaa gataacctgc cgcgcctgca
    19201 aaccgtgttg tttcagcaac agctgggtac actgcgcgac aaaactgacc gcatccaggc
    19261 gctggctggc tggattgctg aacagattgg cgctgacgtt aaccacgcaa cccgtgcggg
    19321 cctgctgtcc aagtgcgacc tgatgaccaa catggtcttc gagttcaccg acacccaggg
    19381 cgttatgggg atgcactacg cgcgtcacga tggcgaagcg gaagatgtgg cagtggcgct
    19441 gaacgagcag tatcagccgc gctttgccgg tgatgacctg ccgtctaacc cggtagcctg
    19501 tgcgctggcg attgctgaca agatggatac tctggcgggt atcttcggta tcggccagca
    19561 tccgaaaggc gacaaagacc cgtttgcgtt gcgtcgtgcc gcacttggcg tgctgcgtat
    19621 tatcgttgag aagaacctca atcttgacct gcaaaccctg accgaagaag cagtgcgtct
    19681 gtatggcgat aagctgacta atgccaacgt ggttgatgat gttatcgact ttatgctcgg
    19741 tcgcttccgc gcctggtatc aggacgaagg ttacaccgtt gacaccatcc aggcggtact
    19801 ggcgcgtcgt ccgactcgtc cggctgattt cgatgcccgt atgaaagcgg tatcgcactt
    19861 ccgtaccctg gaagcagctg cagcactggc ggcggcgaac aagcgtgtat ctaacatcct
    19921 ggcgaaatct gatgaagtgc tgagcgatcg cgtgaatgcc tctactctga aagagccgga
    19981 agaaattaaa ctggcgatgc aggttgtggt gctacgtgac aagctggagc cgtactttgc
    20041 ggaaggtcgt taccaggatg cgctggtcga actggctgag ctgcgtgaac cggtcgatgc
    20101 tttcttcgat aaagtgatgg tcatggttga tgacaaagaa ttgcgaatca accgtcttac
    20161 catgctggag aaactgcgcg aattattcct gcgcgttgcg gatatttcgc tgttacagta
    20221 ataatgccgt tattaaatag cctgccatct ggcaggcttt ttttatcgct aaataataca
    20281 gcaaccttta a
//
LOCUS       DAZFRG010000069        20211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFRG010000069 DAZFRG010000000
VERSION     DAZFRG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.069"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..1499
                     /gene="gspA"
                     /locus_tag="ACHKUC_001964"
     CDS             30..1499
                     /gene="gspA"
                     /locus_tag="ACHKUC_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO6618221.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTRVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTAPLPVPAWLIPVTPV
                     AKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1501..1920
                     /gene="gspB"
                     /locus_tag="ACHKUC_001965"
     CDS             1501..1920
                     /gene="gspB"
                     /locus_tag="ACHKUC_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO6618222.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2158..2469
                     /gene="rpsJ"
                     /locus_tag="ACHKUC_001966"
     CDS             2158..2469
                     /gene="rpsJ"
                     /locus_tag="ACHKUC_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO6618223.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2502..3131
                     /gene="rplC"
                     /locus_tag="ACHKUC_001967"
     CDS             2502..3131
                     /gene="rplC"
                     /locus_tag="ACHKUC_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO6618224.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGSSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3142..3747
                     /gene="rplD"
                     /locus_tag="ACHKUC_001968"
     CDS             3142..3747
                     /gene="rplD"
                     /locus_tag="ACHKUC_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO6618225.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3744..4046
                     /gene="rplW"
                     /locus_tag="ACHKUC_001969"
     CDS             3744..4046
                     /gene="rplW"
                     /locus_tag="ACHKUC_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO6618226.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4064..4885
                     /gene="rplB"
                     /locus_tag="ACHKUC_001970"
     CDS             4064..4885
                     /gene="rplB"
                     /locus_tag="ACHKUC_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO6618227.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4902..5180
                     /gene="rpsS"
                     /locus_tag="ACHKUC_001971"
     CDS             4902..5180
                     /gene="rpsS"
                     /locus_tag="ACHKUC_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO6618228.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5195..5527
                     /gene="rplV"
                     /locus_tag="ACHKUC_001972"
     CDS             5195..5527
                     /gene="rplV"
                     /locus_tag="ACHKUC_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO6618229.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5545..6246
                     /gene="rpsC"
                     /locus_tag="ACHKUC_001973"
     CDS             5545..6246
                     /gene="rpsC"
                     /locus_tag="ACHKUC_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO6618230.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6259..6669
                     /gene="rplP"
                     /locus_tag="ACHKUC_001974"
     CDS             6259..6669
                     /gene="rplP"
                     /locus_tag="ACHKUC_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO6618231.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6669..6860
                     /gene="rpmC"
                     /locus_tag="ACHKUC_001975"
     CDS             6669..6860
                     /gene="rpmC"
                     /locus_tag="ACHKUC_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO6618232.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6860..7114
                     /gene="rpsQ"
                     /locus_tag="ACHKUC_001976"
     CDS             6860..7114
                     /gene="rpsQ"
                     /locus_tag="ACHKUC_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO6618233.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7279..7650
                     /gene="rplN"
                     /locus_tag="ACHKUC_001977"
     CDS             7279..7650
                     /gene="rplN"
                     /locus_tag="ACHKUC_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO6618234.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7661..7975
                     /gene="rplX"
                     /locus_tag="ACHKUC_001978"
     CDS             7661..7975
                     /gene="rplX"
                     /locus_tag="ACHKUC_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO6618235.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7990..8529
                     /gene="rplE"
                     /locus_tag="ACHKUC_001979"
     CDS             7990..8529
                     /gene="rplE"
                     /locus_tag="ACHKUC_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO6618236.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8544..8849
                     /gene="rpsN"
                     /locus_tag="ACHKUC_001980"
     CDS             8544..8849
                     /gene="rpsN"
                     /locus_tag="ACHKUC_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO6618237.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8883..9275
                     /gene="rpsH"
                     /locus_tag="ACHKUC_001981"
     CDS             8883..9275
                     /gene="rpsH"
                     /locus_tag="ACHKUC_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO6618238.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9288..9821
                     /gene="rplF"
                     /locus_tag="ACHKUC_001982"
     CDS             9288..9821
                     /gene="rplF"
                     /locus_tag="ACHKUC_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO6618239.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9831..10184
                     /gene="rplR"
                     /locus_tag="ACHKUC_001983"
     CDS             9831..10184
                     /gene="rplR"
                     /locus_tag="ACHKUC_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO6618240.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10199..10702
                     /gene="rpsE"
                     /locus_tag="ACHKUC_001984"
     CDS             10199..10702
                     /gene="rpsE"
                     /locus_tag="ACHKUC_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO6618241.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10706..10885
                     /gene="rpmD"
                     /locus_tag="ACHKUC_001985"
     CDS             10706..10885
                     /gene="rpmD"
                     /locus_tag="ACHKUC_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO6618242.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10889..11323
                     /gene="rplO"
                     /locus_tag="ACHKUC_001986"
     CDS             10889..11323
                     /gene="rplO"
                     /locus_tag="ACHKUC_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO6618243.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11331..12662
                     /gene="secY"
                     /locus_tag="ACHKUC_001987"
     CDS             11331..12662
                     /gene="secY"
                     /locus_tag="ACHKUC_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO6618244.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12694..12810
                     /gene="rpmJ"
                     /locus_tag="ACHKUC_001988"
     CDS             12694..12810
                     /gene="rpmJ"
                     /locus_tag="ACHKUC_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO6618245.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12957..13313
                     /gene="rpsM"
                     /locus_tag="ACHKUC_001989"
     CDS             12957..13313
                     /gene="rpsM"
                     /locus_tag="ACHKUC_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO6618246.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13330..13719
                     /gene="rpsK"
                     /locus_tag="ACHKUC_001990"
     CDS             13330..13719
                     /gene="rpsK"
                     /locus_tag="ACHKUC_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO6618247.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13753..14373
                     /gene="rpsD"
                     /locus_tag="ACHKUC_001991"
     CDS             13753..14373
                     /gene="rpsD"
                     /locus_tag="ACHKUC_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO6618248.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14399..15388
                     /gene="rpoA"
                     /locus_tag="ACHKUC_001992"
     CDS             14399..15388
                     /gene="rpoA"
                     /locus_tag="ACHKUC_001992"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO6618249.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15429..15812
                     /gene="rplQ"
                     /locus_tag="ACHKUC_001993"
     CDS             15429..15812
                     /gene="rplQ"
                     /locus_tag="ACHKUC_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO6618250.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15919..16287
                     /locus_tag="ACHKUC_001994"
     CDS             15919..16287
                     /locus_tag="ACHKUC_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO6618251.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16298..16723
                     /gene="zntR"
                     /locus_tag="ACHKUC_001995"
     CDS             16298..16723
                     /gene="zntR"
                     /locus_tag="ACHKUC_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO6618252.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16779..16997
                     /gene="arfA"
                     /locus_tag="ACHKUC_001996"
     CDS             16779..16997
                     /gene="arfA"
                     /locus_tag="ACHKUC_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO6618253.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(16994..17407)
                     /gene="mscL"
                     /locus_tag="ACHKUC_001997"
     CDS             complement(16994..17407)
                     /gene="mscL"
                     /locus_tag="ACHKUC_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO6618254.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17537..18913)
                     /gene="trkA"
                     /locus_tag="ACHKUC_001998"
     CDS             complement(17537..18913)
                     /gene="trkA"
                     /locus_tag="ACHKUC_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO6618255.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQESVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18935..>20211)
                     /gene="rsmB"
                     /locus_tag="ACHKUC_001999"
     CDS             complement(18935..>20211)
                     /gene="rsmB"
                     /locus_tag="ACHKUC_001999"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO6618256.1"
                     /translation="NLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALLQELCF
                     GVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAETVEGAV
                     AIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQWQSIV
                     EANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVHALPGF
                     EEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAVDIDEQ
                     RLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRHPDIKW
                     LRRDRDIPELAQLQSEILDAIWSHLKSGGTLVYATCSMLPEENSLQIKAFLQRTADAE
                     LCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
BASE COUNT         5192 a         4754 c         5335 g         4930 t
ORIGIN      
        1 gataatcaat aattaaaaca ggaagttcta tgtctacgag aagagaagtc attctttcct
       61 ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt gttgggtgag gctggaagtg
      121 gaaaaacctg gctggcgcag caactacaaa aagataaaca tcgccgtgtg attactttaa
      181 gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt taccgacgat aatgcggctg
      241 aacagggctg ccgtgacagc gcctggacgc gagatgagat ggcgggtcaa ttactgcatg
      301 cacttcatcg aactgatagt cgctgccctc ttattattat tgaaaatgct cacctgaatc
      361 accgccgcat acttgatgat ctccaacgcg cgatatctct tattcctgac ggtcaatttt
      421 tgctgatcgg cagacccgat cgaaaagttg aacgtgattt taaaaaacag ggcattgaac
      481 ttgtctcgac aggtcgtctg acggagcacg agcttaaggc aagcatcctt gaaggtcaga
      541 atattgacca gcccgatctc cttttaaccg ccagagttct gaaacggata gctttattat
      601 gtcggggaga tcgcagaaag ctggcgcttg ccggcgaaac aattagctta ttacagcaag
      661 ccgagcagac ccgcgttttc actgcaaaac aatggcgaat gatttaccgc gtattaggtg
      721 ataaccgccc ccggaaaatg cagcttgccg tggtgatgag tggaaccatt atcgcactta
      781 cctgtggttg gctactgctg tcctctttca cagctccgtt acccgttcct gcgtggctga
      841 tacccgtaac gccagtagct aaacaagata tgactaagga tattgcccat gtggtgatgc
      901 gcgatagtga ggcgctaagc gtgttatacg gcgtatgggg gtacgaggtc ccagccgatt
      961 cggcctggtg cgatcaggca gtaagagcgg ggctggtatg taaaagtggg aatgcttctt
     1021 tacaaacatt ggtggatcag aatttgccct ggatcgcatc gcttaaagtc ggggacaaaa
     1081 aattgcctgt tgtggtcgtt cgcgtcggtg atgctaccgt tgatgtactc gtagggcagc
     1141 aaacctggac gctgacacat aaatggtttg agttggtatg gacgggtgat tatcttctgt
     1201 tgtggaaaat gtcgccggaa ggggagagta ccattacgcg tgatagcagc gaggaagaga
     1261 ttctctggct ggaaacgatg ttaaaccgcg cattgcatat ttcgacagaa tcttcggcag
     1321 agtggcgtcc cttgttggta gaaaaaataa agcaatttca aaaaagccat cacttgaaaa
     1381 ctgacggtgt tgttggcttt tcaacgttag tacatctctg gcaggtggcg ggggagagtg
     1441 cctacttata tcgggatgaa gcaaatattt ccccagaaac aacagtgaag gggaaatgac
     1501 atgtttgaat tctatatcgc cgcgcgtgag cagaaagaaa cgggtcaccc tggtatcttc
     1561 tcccgacaaa aacacagcac cataatatat gtcatttgtt tattgctgat ttgcctgtgg
     1621 tttgcaggaa tggtgttggt tggtgggtat gcccggcagc tatgggcact ttggatagta
     1681 aaagcagaag tcactgtaga tgctgaaacg cccgctttta aacaatcaac acaacactat
     1741 tttttcaaaa aacagccact ccccgtcgtc gaatccgtcg aggaggaaga tgaaccaggc
     1801 gtcgcggttg agaatgcccc ctcttcttct gaagacgaag aaaatactgt agaagaaagc
     1861 gacgagaaag ccggtttaag agagcgtgtc aaaaatgcgc tgaacgaact tgagagataa
     1921 cccaaaggct gtttacttta ctagcaatac gcttgcgttc ggtggttaag tatgtataat
     1981 gcgcgggctt gtcgtagttg acagcaggtt caatctgaac ctcagttagc cgaatttggc
     2041 tacctaacaa tgctcccaat cggggagcta cgtaagaacg gttacactct cccatcaatc
     2101 gtaatgggtc tgaggagtaa tcattttcgt ttataaaata attggagctc tggtctcatg
     2161 cagaaccaaa gaatccgtat ccgcctgaaa gcgtttgatc atcgtctgat cgatcaagca
     2221 accgcggaaa tcgtcgagac tgctaagcgc actggtgcgc aggtccgtgg tccgatcccg
     2281 ctgccgacac gcaaagagcg cttcactgtt ctgatctccc cgcacgtcaa caaagacgcg
     2341 cgcgatcagt acgaaatccg tactcacttg cgtctggttg acatcgttga gccaaccgag
     2401 aaaaccgttg atgctctgat gcgtctggat ctggctgccg gtgtagacgt gcagatcagc
     2461 ctgggttaat caggtcattg agcgattgag aggttgaaac aatgattggt ttagtcggta
     2521 aaaaagtggg tatgacccgt atcttcacag aagacggcgt ttctatccca gtaaccgtaa
     2581 tcgaagttga agcaaaccgc gttactcagg ttaaagacct ggctaacgat ggctaccgtg
     2641 ctattcaggt gaccaccggt gctaaaaaag ctaaccgtgt gaccaagcct gaagctggac
     2701 acttcgctaa agctggcgta gaagctggcc gtggtctgtg ggaattccgc ctggctgaag
     2761 gcgaagagtt cactgtaggt cagagcatta gcgttgaact gtttgctgac gttaaaaaag
     2821 ttgacgtaac tggcagctct aaaggtaaag gtttcgcagg taccgttaag cgctggaact
     2881 tccgtaccca ggacgctact cacggtaact ccttgtctca ccgcgttccg ggttctatcg
     2941 gtcagaacca gactccgggc aaagtgttca aaggcaagaa aatggcaggt cagatgggta
     3001 acgaacgtgt aaccgttcag agccttgacg tagtacgcgt tgacgctgag cgcaacctgc
     3061 tgctggttaa aggcgctgtc ccgggtgcaa ccggtagcga cctgatcgtt aaaccagctg
     3121 tgaaggcgta aggagatagc aatggaatta gtattgaaag acgcgcagag cgcgctgact
     3181 gtttccgaaa ctaccttcgg tcgtgatttc aacgaagcgc tggttcacca ggttgttgtt
     3241 gcttatgcag ctggtgctcg tcagggtact cgtgctcaga agactcgtgc tgaagtaact
     3301 ggttccggta aaaaaccgtg gcgccagaaa ggcaccggcc gtgcgcgttc tggttctatc
     3361 aagagcccga tctggcgttc tggtggcgtg acctttgctg ctcgtccgca ggaccacagt
     3421 caaaaagtta acaagaagat gtaccgcggc gcgctgaaaa gcatcctgtc cgaactggta
     3481 cgtcaggatc gtctgatcgt tgtcgagaag ttctctgtag aagcgccgaa aactaagctg
     3541 ctggcacaga aactgaaaga catggctctg gaagatgtgc tgatcatcac cggtgagctg
     3601 gacgagaacc tgttcctggc tgcgcgcaac ctgcacaagg ttgacgtacg cgatgcaact
     3661 ggtatcgacc cggttagcct gatcgccttc gacaaagtcg taatgactgc tgatgctgtt
     3721 aagcaagttg aggagatgct ggcatgattc gtgaagaacg tctgctgaag gtgctgcgtg
     3781 caccgcacgt ttctgaaaaa gcgtctactg cgatggaaaa atccaacacc atcgtactca
     3841 aagttgctaa agacgcgacc aaagcagaaa tcaaagctgc tgtgcagaaa ctgtttgaag
     3901 tcgaagtcga agtcgttaac accctggtag ttaaagggaa agttaaacgt cacggacagc
     3961 gtatcggtcg tcgtagcgac tggaaaaaag cttacgtcac cctgaaagaa ggccagaatc
     4021 tggacttcgt tggcggcgct gagtaagtcg gaggagtaat acaatggcag ttgttaaatg
     4081 taaaccgaca tctccgggtc gtcgccacgt agttaaagtg gttaaccctg agctgcacaa
     4141 gggcaaacct tttgctccgt tgctggaaaa aaacagcaaa tccggtggtc gtaacaacaa
     4201 tggccgtatc accactcgtc atatcggtgg tggccacaag caggcttacc gtattgttga
     4261 cttcaaacgc aacaaagacg gtatcccggc agttgttgaa cgtcttgagt acgatccgaa
     4321 ccgttccgcg aacatcgcgc tggttctgta caaagacggt gaacgccgtt acatcctggc
     4381 ccctaaaggc ctgaaagctg gcgaccagat tcagtctggc gttgatgctg caatcaaacc
     4441 aggtaacacc ctgccgatgc gcaacatccc ggttggttct actgttcata acgtagaaat
     4501 gaaaccaggt aaaggcggtc agctggcacg ttccgctggt acttacgttc agatcgttgc
     4561 tcgtgatggt gcttatgtca ccctgcgtct gcgttctggt gaaatgcgta aagtagaagc
     4621 agactgccgt gcaactctgg gcgaagttgg caatgctgag catatgctgc gcgttctggg
     4681 taaagcaggt gctgcacgct ggcgtggtgt tcgtccgacc gttcgcggta ccgcgatgaa
     4741 cccggtagac cacccacatg gtggtggtga aggtcgtaac tttggtaagc acccggtaac
     4801 tccgtggggc gttcagacca aaggtaagaa gacccgcagc aacaagcgta ctgataaatt
     4861 catcgtacgt cgccgtagca aataatttta gaggataagc catgccacgt tctctcaaga
     4921 aaggtccttt tattgacctg cacttgctga agaaggtaga gaaagcggtg gaaagcggag
     4981 acaagaagcc cctgcgcact tggtcccgtc gttcaacgat ctttcctaac atgatcggtt
     5041 tgaccatcgc tgtccataat ggtcgtcagc acgttccggt atttgtaacc gacgaaatgg
     5101 tcggtcacaa actgggtgaa ttcgcaccga ctcgtactta tcgcggccac gctgctgata
     5161 aaaaagcgaa gaagaaataa ggtaggagga agagatggaa actatcgcta aacatcgcca
     5221 tgctcgttct tctgctcaga aggttcgcct tgttgctgac ctgattcgcg gtaagaaagt
     5281 gtcgcaggct ctggatattt tgacctacac caacaagaaa gcggctgtac tggtcaagaa
     5341 agttctggaa tctgccattg ctaacgctga acacaacgat ggcgctgaca ttgacgatct
     5401 gaaagttacg aaaattttcg tagacgaagg cccgagcatg aagcgcatta tgccgcgtgc
     5461 aaaaggtcgt gcagatcgca tcctgaagcg caccagccac atcactgtgg ttgtgtccga
     5521 tcgctgagac tctggagact agcaatgggt cagaaagtac atcctaatgg tattcgcctg
     5581 ggtattgtaa aaccatggaa ctctacctgg tttgcgaaca ccaaagaatt cgctgacaac
     5641 ctggacagcg attttaaagt acgtcagtac ctgactaagg aactggctaa agcgtccgta
     5701 tctcgtatcg ttatcgagcg tccggctaag agcatccgtg taaccattca cactgctcgc
     5761 ccgggtatcg ttatcggtaa aaaaggtgaa gacgtagaaa aactgcgtaa ggtcgtagcg
     5821 gacatcgctg gcgttcctgc acagatcaac atcgccgaag ttcgtaagcc tgaactggac
     5881 gcaaaactgg ttgctgacag catcacttct cagctggaac gtcgcgttat gttccgtcgt
     5941 gctatgaagc gtgctgtaca gaacgcaatg cgtctgggcg ctaaaggtat taaagttgaa
     6001 gttagcggcc gtctgggcgg cgcggaaatc gcacgtaccg aatggtaccg cgaaggtcgc
     6061 gtaccgctgc acactctgcg tgctgacatc gactacaaca cctctgaagc gcacaccact
     6121 tacggtgtaa tcggcgttaa agtgtggatc ttcaaaggcg agatcctggg tggtatggct
     6181 gctgttgaac aaccggaaaa accggctgct cagcctaaaa agcagcagcg taaaggccgt
     6241 aaataaggag cgtcgctgat gttacaacca aagcgtacaa aattccgtaa aatgcacaaa
     6301 ggccgtaacc gcggtctggc gcagggtacg gatgttagct tcggcagctt cggtctgaaa
     6361 gctgttggcc gtggtcgtct gactgcacgt cagatcgaag cagcacgtcg tgctatgacc
     6421 cgtgcagtta agcgtcaagg taagatctgg atccgtgtgt tcccggacaa accgatcact
     6481 gaaaagccgc tggcagtgcg tatgggtaaa ggtaaaggta acgtggagta ttgggttgcc
     6541 ttgattcagc cgggtaaagt cctgtatgaa atggacggtg ttccggaaga gctggcccgt
     6601 gaagcattca agctggcagc agcgaaactg ccgattaaaa ccacctttgt aactaagacg
     6661 gtgatgtaat gaaagcaaaa gagctgcgtg agaagagcgt tgaagagctg aacaccgagc
     6721 tgctgaacct gctgcgtgag cagttcaacc tgcgtatgca ggctgcaagt ggccagctgc
     6781 aacagtctca cctgttgaag caagtgcgtc gcgatgtcgc acgcgttaag actttactga
     6841 acgagaaggc gggtgcgtaa tgaccgataa aatccgtact ctgcaaggtc gcgttgttag
     6901 cgacaaaatg gagaaatcca ttgttgttgc tatcgaacgt tttgtgaaac acccgatcta
     6961 cggtaaattc atcaagcgta cgaccaaact gcacgtacat gacgagaaca acgaatgcgg
     7021 tatcggtgac gtggttgaaa tccgcgaatg ccgtccgctg tccaagacta aatcctggac
     7081 gctggttcgc gttgtagaga aagcggttct gtaatacagt acactctctc gatacgaata
     7141 aacggctcag aaatgagccg tttatttttt ctacccatat ccttgaagcg gtgttataat
     7201 gccgcgccct cgatatgggg atttttaacg acctgatttt cgggtctcag tagtagttga
     7261 cattagcgga gcactaaaat gatccaagaa cagactatgc tgaacgtcgc cgacaactcc
     7321 ggtgcacgtc gcgtaatgtg tatcaaggtt ctgggtggct cgcaccgtcg ctacgcaggc
     7381 gtaggcgaca tcatcaagat caccatcaaa gaagcaattc cgcgtggtaa ggtcaaaaaa
     7441 ggtgatgtgc tgaaggcggt agtggtgcgc accaagaagg gtgttcgtcg cccggacggt
     7501 tctgtcattc gcttcgatgg taatgcttgt gttcttctga acaacaacag cgagcagcct
     7561 atcggtacgc gtatttttgg gccggtaact cgtgagcttc gtagtgagaa gttcatgaaa
     7621 attatctctc tggcaccaga agtactctaa ggagcgaatc atggcagcga aaatccgtcg
     7681 tgatgacgaa gttatcgtgt taaccggtaa agataaaggt aaacgcggta aagttaagaa
     7741 tgtcctgtct tccggcaagg tcattgttga aggtatcaac ctggttaaga aacatcagaa
     7801 gccggttccg gccctgaacc aaccgggtgg catcgttgaa aaagaagccg ctattcaggt
     7861 ttccaacgta gcaatcttca atgcgacaac cggcaaggct gaccgtgtag gctttagatt
     7921 cgaagacggt aaaaaagtcc gtttcttcaa gtctaacagc gaaactatca agtaatttgg
     7981 agtagtacga tggcgaaact gcatgattac tacaaagacg aagtagttaa aaaactcatg
     8041 actgagttta actacaattc tgtcatgcaa gtccctcggg tcgagaagat caccctgaac
     8101 atgggtgttg gtgaagcgat cgctgacaaa aaactgctgg ataacgcagc agcagacctg
     8161 gcagcaatct ccggtcaaaa accgctgatc accaaagcac gcaaatctgt tgcaggcttc
     8221 aaaatccgtc agggctatcc gatcggctgt aaagtaactc tgcgtggcga acgcatgtgg
     8281 gagttctttg agcgcctgat cactattgct gtacctcgta tccgtgactt ccgtggcctg
     8341 tccgctaagt ctttcgacgg tcgtggtaac tacagcatgg gtgtccgtga gcagatcatc
     8401 ttcccagaaa tcgactacga taaagtcgac cgcgttcgtg gtttggatat taccattacc
     8461 actactgcga aatctgacga agaaggccgc gctctgctgg ctgcctttga cttcccgttc
     8521 cgcaagtaag gtagggttac taaatggcta agcaatcaat gaaagcacgc gaagtaaaac
     8581 gcgtagcttt agctgataaa tacttcgcga aacgcgctga actgaaagcg atcatctctg
     8641 atgtgaacgc ttccgacgaa gatcgttgga acgctgttct caagctgcag actctgccgc
     8701 gtgattccag cccgtctcgt cagcgtaacc gctgccgtca aacaggtcgt ccgcatggtt
     8761 tcctgcggaa gttcgggttg agccgtatta aggtccgtga agccgctatg cgcggtgaaa
     8821 tcccgggtct gaaaaaggct agctggtaat tgtcaccaat tgaatcacgg gagtaaagac
     8881 agatgagcat gcaagatccg atcgcggata tgctgacccg tatccgtaac ggtcaggccg
     8941 cgaacaaagc tgcggtcacc atgccttcct ccaagctgaa agtggcaatc gccaacgtgc
     9001 tgaaggaaga aggttttatt gaagatttta aagttgaagg cgacaccaag cctgaactgg
     9061 aacttactct gaagtatttc cagggcaaag ctgttgtaga aagcattcag cgtgtcagcc
     9121 gcccaggtct gcgcatctat aaacgtaaag atgagctgcc gaaagttatg gcgggtctgg
     9181 gtatcgcagt tgtttctacc tctaaaggtg ttatgactga tcgtgcagcg cgccaggctg
     9241 gtcttggtgg cgaaattatc tgctacgtag cctaatcgga ggaaaaaatg tctcgtgttg
     9301 ctaaagcacc ggtcgttgtt cctgccggcg ttgacgtaaa aatcaacggt caggttatta
     9361 cgatcaaagg taaaaacggc gagctgactc gtactctcaa cgatgctgtt gaagttaaac
     9421 atgcagataa taccctgacc ttcggtccgc gtgatggtta cgcagacggt tgggcacagg
     9481 ctggtaccgc gcgtgccctg ctgaactcaa tggttatcgg tgttaccgaa ggcttcacta
     9541 agaagctgca gctggttggt gtaggttacc gtgcagcggt taaaggcaat gtgattaacc
     9601 tgtctctggg tttctctcat cctgttgacc atcagctgcc tgcgggtatc actgctgaat
     9661 gtccgactca gactgaaatc gtgctgaaag gcgctgataa gcaggtgatc ggccaggttg
     9721 cagcggatct gcgcgcctac cgtcgtcctg agccttataa aggcaagggt gttcgttacg
     9781 ccgacgaagt cgtgcgtacc aaagaggcta agaagaagta aggtaacact atggataaga
     9841 aatctgctcg tatccgtcgt gcgacccgcg cacgccgcaa gctccaggag ctgggcgcaa
     9901 ctcgcctggt ggtacatcgt accccgcgtc acatttacgc acaggtaatt gcaccgaacg
     9961 gttctgaagt tctggtagct gcttctactg tagaaaaagc tatcgctgaa caactgaagt
    10021 acaccggtaa caaagacgcg gctgcagctg tgggtaaagc tgtcgctgaa cgcgctctgg
    10081 aaaaaggcat caaagatgta tcctttgacc gttccgggtt ccaatatcat ggtcgtgtcc
    10141 aggcactggc agatgctgcc cgtgaagctg gccttcagtt ctaaggtaga ggtgtaagat
    10201 ggctcacatc gaaaaacaag ctggcgaact gcaggaaaag ctgatcgcgg taaaccgcgt
    10261 atctaaaacc gttaaaggtg gtcgtatttt ctccttcaca gctctgactg tagttggcga
    10321 tggtaacggt cgcgttggtt ttggttacgg taaagcgcgt gaagttccag cagcgatcca
    10381 gaaagcgatg gaaaaagccc gtcgcaatat gattaacgtc gcgctgaata acggtactct
    10441 gcaacaccct gttaaaggtg ttcatacggg ttctcgcgta ttcatgcagc cggcttccga
    10501 aggtaccggt atcatcgccg gtggtgcaat gcgcgccgtt ctggaagtcg ctggggttca
    10561 taacgttctg gctaaagcgt atggttccac caacccgatt aacgtggttc gtgcaactat
    10621 cgatggcctg gaaaatatga attctccaga aatggtcgct gccaagcgtg gtaaatccgt
    10681 tgaagaaatt ctggggaaat aaaccatggc aaagactatt aaaattactc aaacccgcag
    10741 tgcaatcggt cgtctgccga aacacaaggc aacgctgctt ggcctgggtc tgcgtcgtat
    10801 tggtcacacc gtagagcgcg aggatactcc tgctattcgc ggtatgatca acgcggtttc
    10861 cttcatggtt aaagttgagg agtaagagat gcgtttaaat actctgtctc cggccgaagg
    10921 ctccaaaaag gcgggtaaac gcctgggtcg tggtatcggt tctggcctcg gtaaaaccgg
    10981 tggtcgtggt cacaaaggtc agaaatctcg ttctggcggt ggcgtacgtc gcggtttcga
    11041 gggtggtcag atgcctctgt accgtcgtct gccgaaattc ggcttcactt ctcgtaaagc
    11101 agcgattaca gccgaagttc gtctgtctga cctggctaaa gtagaaggcg gtgttgtaga
    11161 cctgaacacg ctgaaagcgg ctaacattat cggtatccag atcgagttcg cgaaagtgat
    11221 cctggctggc gaagtaacga ctccggtaac tgttcgtggc ctgcgtgtta ctaaaggcgc
    11281 tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa taagtagcag atggctaaac
    11341 aaccgggatt agattttcaa agtgccaaag gtggcttagg cgagctgaaa cgcagactgc
    11401 tgtttgttat cggtgcgctg attgtgttcc gtattggctc ttttattccg atccctggta
    11461 ttgatgccgc tgtacttgcc aaactgcttg agcaacagcg aggcaccatc attgagatgt
    11521 ttaacatgtt ctctggtggt gctctcagcc gtgcttctat ctttgctctg gggatcatgc
    11581 cgtatatttc ggcgtcgatc attatccagc tactgacggt ggttcatcca acgttggcag
    11641 aaattaagaa agaaggggag tctggtcgtc gtaagatcag ccagtacacc cgctacggta
    11701 ctctggtgct ggcaatattc cagtcgatcg gtattgctac cggtctgccg aatatgcctg
    11761 gtatgcaagg cctggtcatt aacccgggct ttgcattcta cttcaccgct gttgtaagtc
    11821 tggtcacagg aaccatgttc ctgatgtggt tgggcgaaca gatcactgaa cgaggtatcg
    11881 gcaacggtat ttcaatcatt atcttcgccg gtattgtcgc gggactcccg ccagccattg
    11941 cccatactat cgagcaagcg cgtcaaggcg acctgcactt cctcgtgttg ctgttggttg
    12001 cagtattagt atttgcagtg acgttctttg ttgtatttgt tgagcgtggt caacgccgca
    12061 ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt ctatgctgca cagagcacac
    12121 atttaccgct gaaagtgaat atggcggggg taatcccggc aatcttcgct tccagtatta
    12181 ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg tactggttgg aactggctga
    12241 caacaatttc gctgtatttg cagcctgggc aaccgcttta tgtgttactc tatgcgtctg
    12301 caatcatctt cttctgtttc ttctacacgg cgttggtttt caacccgcgt gaaacagcag
    12361 ataacctgaa gaagtccggt gcatttgtac caggaattcg tccgggagag caaacggcga
    12421 agtatatcga taaagtaatg acccgcctga ccttggttgg tgcgctgtac attaccttta
    12481 tctgcctgat cccggagttc atgcgtgatg caatgaaagt accgttctac ttcggtggga
    12541 cctcactgct tatcgttgtt gtcgtgatta tggactttat ggctcaagtg caaactctga
    12601 tgatgtccag tcagtatgag tctgcattga agaaggcgaa cctgaaaggc tacggccgat
    12661 aattggtcgc ccgagaagtt acggagagta aaaatgaaag ttcgtgcttc cgtcaagaaa
    12721 ttatgccgta actgcaaaat cgttaagcgt gatggtgtca tccgtgtgat ttgcagtgcc
    12781 gagccgaagc ataaacagcg ccaaggctga ttttttcgca tatttttctt gcaaagttgg
    12841 gttgagctgg ctagattagc cagccaatct tttgtatgtc tgtgcgtttc catttgagta
    12901 tcctgaaaac gggcttttca gcatggaacg tacatattaa atagtaggag tgcatagtgg
    12961 cccgtatagc aggcattaac attcctgatc ataagcatgc cgtaatcgca ttaacttcga
    13021 tttatggcgt cggcaagacc cgttctaaag ccatcctggc tgcagcgggt atcgctgaag
    13081 atgttaagat cagtgagctg tctgaaggac aaatcgacac gctgcgtgac gaagttgcca
    13141 aatttgtcgt tgaaggtgat ctgcgccgtg aaatcagcat gagcatcaag cgcctgatgg
    13201 atcttggttg ctatcgcggt ttgcgtcatc gtcgtggtct cccggttcgc ggtcagcgta
    13261 ccaagaccaa cgcacgtacc cgtaagggtc cgcgcaaacc gatcaagaaa taatcggggt
    13321 gattgaataa tggcaaaggc accaattcgt gcacgtaaac gtgtaagaaa acaagtctct
    13381 gacggcgtgg ctcatatcca tgcttctttc aacaacacca tcgtgactat cactgatcgt
    13441 cagggtaacg cgttgggttg ggcaacagcc ggtggttccg gtttccgtgg ttctcgcaaa
    13501 tccactccgt ttgcagcaca ggttgcagca gagcgttgcg ctgacgccgt gaaagaatac
    13561 ggcatcaaga atctggaagt tatggttaaa ggtccgggtc caggccgcga atctactatt
    13621 cgtgctctga acgccgcagg tttccgcatc actaacatta ctgatgtgac tccgatccct
    13681 cataacggtt gtcgtccgcc gaaaaaacgt cgcgtataac gcctcgtttt ccaggtttgt
    13741 tggagaaaga aaatggcaag atatttgggt cctaagctca agctgagccg tcgtgagggc
    13801 accgacttat tccttaagtc tggcgttcgc gcgatcgata ccaagtgtaa aattgaacaa
    13861 gctcctggcc agcacggtgc gcgtaaaccg cgtctgtctg actatggtgt gcagttgcgt
    13921 gaaaagcaaa aagttcgccg tatctatggt gtgctggagc gtcagttccg taactactac
    13981 aaagaagcag cacgtctgaa aggcaacacc ggtgaaaacc tgttggctct gctggaaggt
    14041 cgtctggaca acgttgtata ccgtatgggc ttcggtgcca ctcgtgcaga agcacgtcag
    14101 ctggttagcc ataaagcaat tatggtaaac ggtcgtgttg ttaacatcgc ttcttatcag
    14161 gttagtccga atgacgttgt aagcattcgt gagaaagcga agaagcagtc tcgcgtgaaa
    14221 gccgctctgg agctggctga gcagcgtgaa aagccaacct ggttggaagt tgatgctggc
    14281 aagatggaag gtacgtttaa gcgtaagccg gagcgttctg atctgtctgc ggacattaac
    14341 gaacacctga tcgtcgagct ttactccaag taaagcttag taccaaagag aggacacaat
    14401 gcagggttct gtgacagagt ttctaaaacc gcgcctggtt gatatcgagc aagtgagttc
    14461 gacgcacgcc aaggtgaccc ttgagccttt agagcgtggc tttggccata ctctgggtaa
    14521 cgcactgcgc cgtattctgc tctcatcgat gccgggttgc gcggtgaccg aggttgagat
    14581 tgatggtgta ctacatgagt acagcaccaa agaaggcgtt caggaagata tcctggaaat
    14641 cctgctcaac ctgaaagggc tggcggtgag agttcagggc aaagatgaag ttattcttac
    14701 cttgaataaa tctggcattg gccctgtgac tgcagccgat atcacccacg acggtgatgt
    14761 cgaaatcgtc aagccgcagc acgtgatctg ccacctgacc gatgagaacg cgtctattag
    14821 catgcgtatc aaagttcagc gcggtcgtgg ttatgtgccg gcttctaccc gaattcattc
    14881 ggaagaagat gagcgcccaa tcggccgtct gctggtcgac gcatgctaca gccctgtgga
    14941 gcgtattgcc tacaatgttg aagcagcgcg tgtagaacag cgtaccgacc tggacaagct
    15001 ggtcatcgaa atggaaacca acggcacaat cgatcctgaa gaggcgattc gtcgtgcggc
    15061 aaccattctg gctgaacaac tggaagcttt cgttgactta cgtgatgtac gtcagcctga
    15121 agtgaaagaa gagaaaccag agttcgatcc gatcctgctg cgccctgttg acgatctgga
    15181 attgactgtc cgctctgcta actgccttaa agcagaagct atccactata tcggtgatct
    15241 ggtacagcgt accgaggttg agctccttaa aacgcctaac cttggtaaaa aatctcttac
    15301 tgagattaaa gacgtgctgg cttcccgtgg actgtctctg ggcatgcgcc tggaaaactg
    15361 gccaccggca agcatcgctg acgagtaacc ggatcacagg ttaaggtttt actgagaagg
    15421 ataaggtcat gcgccatcgt aagagtggtc gtcaactgaa ccgcaacagc agccatcgcc
    15481 aggctatgtt ccgcaatatg gcaggttcac tggttcgtca tgaaatcatc aagacgactc
    15541 tgcctaaagc gaaagagctg cgccgcgtag ttgagccgct gattactctt gccaagactg
    15601 atagcgttgc taatcgtcgt ctggcattcg cccgtactcg tgataacgag atcgtggcaa
    15661 aactgtttaa cgaactgggc ccgcgtttcg cgagccgtgc cggtggttac actcgtattc
    15721 tgaagtgtgg cttccgtgca ggcgacaatg cgccgatggc ttacatcgag ctggttgatc
    15781 gttcagagaa agcagaagct gctgcagagt aatctgaagc aacgtaaaaa aacccgcccc
    15841 ggcgggtttt tttatacccg tagcatcccc acttatctac aatagctgta ctctttttat
    15901 tcatcccctg gagtatttat gtggttactt gaccagtggg cagagcgtca tatagcagaa
    15961 gcgcaagcga aaggtgagtt tgataacctg gtaggtagcg gcgaaccatt gatactggat
    16021 gatgattctc atgtgccacc ggaattacgt gcggggtatc gcttgctgaa gaatgctggt
    16081 tgcttaccgc cagaacttga gcataggagg gaagcaatac agcttctgga tattctcaaa
    16141 ggtatccgtc atgatgatcc gcaatatcaa gaggttagtc gtcgattgtc attactggaa
    16201 ttgaagctgc gacaagctgg attgagtacc gattttttac gcggcgatta tgctgacaag
    16261 ttgttgaaca aaatcaacga taactagtgg agtatgtatg tatcgcattg gtgagctggc
    16321 aaaaatggcg gaagtaacac ccgacacgat tcgttattac gaaaagcagc agatgatgga
    16381 gcatgaagta cgcaccgaag gtgggtttcg cctgtatacc gaaagcgatc tccagcgatt
    16441 gaaatttatc cgccatgcca gacaactagg tttcagtctg gagtcgatcc gcgagttgct
    16501 gtcgatccgt atcgatccgg aacaccatac ctgtcaggag tcaaaaggca ttgtgcagga
    16561 acgattgcag gaagtcgaag ctcgtatagc cgagttgcag agtatgcagc gttccttgca
    16621 acgccttaat gatacctgtt gtgggaccgc ccatagcagt gtttattgct cgattcttga
    16681 agctcttgaa caaggggcga gtggcgttaa gagtggctgt tgattttttg cactggcagg
    16741 attacactcg cgccgttaaa taaccaactg gagtttttat gagccgatat cagcatacta
    16801 aagggcagat aaaggataat gcgatagaag cattactaca tgatccctta ttccgacagc
    16861 gagtagagaa aaataagaaa gggaaaggca gttacatgcg aaaaggaaaa catggcaatc
    16921 gggggaactg ggaggccagt ggcaagaaag taaatcactt ttttaccact ggccttctgc
    16981 tttcagacgc ttgttaagag cggttattct gctctttcag caaatcacga atttctgtca
    17041 gtaatacttc ttctttagtt ggtgcaggtg ttgctgctgc tggttcttct tttttccgat
    17101 tcagtttgtt gattagctta atcgccataa agatggcaaa ggccactatc agaaaatcaa
    17161 agacgttttg aatgaagaca ccgtaatgca tcacaacagc agggatatcc ccttgggcct
    17221 cgcgtagcgt gacagcaaac tgtttaaagt cgatcccgcc aattaataag cccagaggtg
    17281 gcatgatgat atcggcaacc agtgaagata caatcttccc gaatgccgct ccgataatga
    17341 cacccactgc caaatccacc acgttcccgc gcatcgcaaa ttcgcgaaat tctttaataa
    17401 tgctcatgtt attctcccta tgaagccgac aaccataagt ctaacaaatg ttaagccatt
    17461 ttcctgccag gaatggattt aattaatcct taataatcaa agggaaataa taaggcgtca
    17521 ttagacgcct tattaattac aagaagaaag gacttggctg gaagagtctt tcgacgtcgg
    17581 taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca atgcgcagat
    17641 tgtcattggc aatcatcaca tcgtttccac gtaccaccgc tccaataatc gttcctggcg
    17701 gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca tcaccgtgag
    17761 caacggcttc aatagcttct gctacgccgc ggcgtaatga ggaaacacca acaatatctg
    17821 cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa atcgcaatat
    17881 cgatcacgct ctcctgaacc agatccacat aagcgcgacg ttggatcaat accatcacct
    17941 ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg tcatcgttgg
    18001 tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt tcttgatccg
    18061 acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg gcagcgcgct
    18121 gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc gccagccctg
    18181 caccgatatt accgccacca accagcatga tccgcttata cggtttttcc agtcgctgta
    18241 attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact tcatcaccag
    18301 cttcaacaat ggtcgaacct tgcggacgaa tggggcgatc gtggcggaaa attgctgcca
    18361 cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta ccaatcagcg
    18421 ggccgccata ataggcttta accacagcca ggctgacttt accctcagcg aagttcacca
    18481 cctgcaatgc gccgggatac tcaatcagtc ggtaaatatt atcgataacc aactgctctg
    18541 gtgcgatcag atgatcaatc ggaacagcat ctgaatgaaa tagcttatcg gcatcgcgca
    18601 cgtagtctgg tgagcggata cgagcgatgc gattaggggt gttgaaaagt gagtaggcta
    18661 cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc atatcggcgt
    18721 cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc acgactcgca
    18781 ggtcaaattt atcctgtaag gtccgcagac gctcaccgtt ggtatcgaca acagtaatat
    18841 cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg gcacccagaa
    18901 tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc ttagcgtaaa
    18961 agaagccgtc gccctcttcg gcaccgggca aattttgttt acccggttgc tctggtgttc
    19021 ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc tgcaggctat
    19081 tctcttccgg taacatcgaa caggtggcat agaccagggt tcctccggat tttaaatgcg
    19141 accagatggc gtcgagaatt tcagactgca actgcgcgag ttctgggata tcgcgatcgc
    19201 ggcgtaacca tttaatatcc ggatggcgac gaattacacc agttgccgaa caaggtgcat
    19261 ctaataaaat gcgatcgaac tgttgctcac cacaccattg ggaagggtaa cggccatcac
    19321 cttgttttac ggttgccttc ataccaaggc gttttaaatt gtcgtaaacg cgagagaggc
    19381 gctgctcatc aatatcgacc gccagaactt gcgcctctgg agcaacctca agaatatgcg
    19441 ttgttttacc gcctggggcg gcgcaaagat ccagaatgtg ttcaccattt tgtggagcaa
    19501 gccaggtcat gcaaccttgt gctgaagcat cctgaacggt aacccatcct tcttcaaaac
    19561 cgggtagcgc atgaacaggt gcaggtgttt ccagctgtac agcatcaggg taatccgcat
    19621 gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg cgggaatgat
    19681 gcgtacgatt aacgcgcagc cacattggcg gacgctggtt attggcttcg acgatggatt
    19741 gccactgctc gggatacgcc ttttgcagac gcttcagcaa ccaggaagga tgcagataac
    19801 gtgcatcact ggcattaaac tcggctaata actcatcttg ctgacgctgg aactgacgta
    19861 atacaccgtt aatcaatccc ttaagttgcg ggcgcttaat tgcaactgcg ccttcaaccg
    19921 tttcagccag cgcggcatga ggtggaatgc gggtataaag cagttgatac aaaccaacca
    19981 taatcaggta atgaacagta cgctgtttgc ctgtcatcgg acgggccatt agcttattaa
    20041 tcagccagtc taactgtgag agcgtacgca gtacgccaaa gcataactcc tggagaagag
    20101 ctttatcttt atcggaaact ttttgctgga gcggtggcag aatgttgctc agtgattgcc
    20161 cttgctcaac gacttgttca atggcctggg ccgccatgct acgtaaatta c
//
LOCUS       DAZFRG010000070        20194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFRG010000070 DAZFRG010000000
VERSION     DAZFRG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.070"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(147..1376)
                     /locus_tag="ACHKUC_002000"
     CDS             complement(147..1376)
                     /locus_tag="ACHKUC_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6618257.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILLFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLVHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            1364..1546
                     /locus_tag="ACHKUC_002001"
     CDS             1364..1546
                     /locus_tag="ACHKUC_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618258.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            1553..2458
                     /gene="tqsA"
                     /locus_tag="ACHKUC_002002"
                     /pseudo
     CDS             1553..2458
                     /gene="tqsA"
                     /locus_tag="ACHKUC_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(2483..3871)
                     /gene="pntB"
                     /locus_tag="ACHKUC_002003"
     CDS             complement(2483..3871)
                     /gene="pntB"
                     /locus_tag="ACHKUC_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO6618259.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3882..5414)
                     /gene="pntA"
                     /locus_tag="ACHKUC_002004"
     CDS             complement(3882..5414)
                     /gene="pntA"
                     /locus_tag="ACHKUC_002004"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO6618260.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            5938..6882
                     /gene="ydgH"
                     /locus_tag="ACHKUC_002005"
     CDS             5938..6882
                     /gene="ydgH"
                     /locus_tag="ACHKUC_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO6618261.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIIQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7068..8450
                     /gene="ydgI"
                     /locus_tag="ACHKUC_002006"
     CDS             7068..8450
                     /gene="ydgI"
                     /locus_tag="ACHKUC_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6618262.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8487..9209
                     /gene="folM"
                     /locus_tag="ACHKUC_002007"
     CDS             8487..9209
                     /gene="folM"
                     /locus_tag="ACHKUC_002007"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO6618263.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPTI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(9206..9541)
                     /gene="ydgC"
                     /locus_tag="ACHKUC_002008"
     CDS             complement(9206..9541)
                     /gene="ydgC"
                     /locus_tag="ACHKUC_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO6618264.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9670..10389
                     /gene="rstA"
                     /locus_tag="ACHKUC_002009"
     CDS             9670..10389
                     /gene="rstA"
                     /locus_tag="ACHKUC_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO6618265.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10393..11694
                     /gene="rstB"
                     /locus_tag="ACHKUC_002010"
     CDS             10393..11694
                     /gene="rstB"
                     /locus_tag="ACHKUC_002010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO6618266.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVEISLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11770..12699
                     /gene="tus"
                     /locus_tag="ACHKUC_002011"
     CDS             11770..12699
                     /gene="tus"
                     /locus_tag="ACHKUC_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO6618267.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEIKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12696..14099)
                     /gene="fumC"
                     /locus_tag="ACHKUC_002012"
     CDS             complement(12696..14099)
                     /gene="fumC"
                     /locus_tag="ACHKUC_002012"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO6618268.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAASKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(14242..15888)
                     /gene="fumB"
                     /locus_tag="ACHKUC_002013"
     CDS             complement(14242..15888)
                     /gene="fumB"
                     /locus_tag="ACHKUC_002013"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6618269.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            16087..17262
                     /gene="manA"
                     /locus_tag="ACHKUC_002014"
     CDS             16087..17262
                     /gene="manA"
                     /locus_tag="ACHKUC_002014"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO6618270.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            17363..18871
                     /gene="ydgA"
                     /locus_tag="ACHKUC_002015"
     CDS             17363..18871
                     /gene="ydgA"
                     /locus_tag="ACHKUC_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6618271.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTVNSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(18916..20181)
                     /gene="uidC"
                     /locus_tag="ACHKUC_002016"
     CDS             complement(18916..20181)
                     /gene="uidC"
                     /locus_tag="ACHKUC_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO6618272.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLRTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         4953 a         5266 c         4853 g         5122 t
ORIGIN      
        1 accagtaaca cataaaaaat cagctaatta taaatccgta cactttttcg cctcttgttc
       61 gtagttgttc cgcattgttg ctcattgttg cacgttgtat ctgttcgcat atccagtatg
      121 cgcatactga aaaaacacga aaaaaattat tttcttctgg ctactggtag cgtggttacg
      181 ttttcatgtg ttcccgccag tatccctaac cgctccgtcc acatatccag cgcattgcgt
      241 ttagcatcca gataacggga atgattataa actcgctgca ttcctggcat ctggtggcct
      301 gtaagctgct ccacgacatg cggatcaacg cctaaatcgt tcagcatggt tgtaaaggtg
      361 cgccggatgt catgcagtga ccagtgaggg tgattaagcc tcctgtgcac taatctgccg
      421 tactgcgata cgctggcctc ctgtttcact tcccccagca ataagcccgt gtgcctgttc
      481 tgctccacca gctgcgtgac gaacagcagg atcgcttccg gtatgggccg gaatattgcg
      541 accttcgttt tgctgtgctc cttcggaacg gtccatagca tttccgtaaa atcccactcc
      601 ccgatctccg ataacctcag ttctaccgtt ctggctccga agacaatcag gaggcggatt
      661 aacgcgacgt agtaagggga aaatattttt ttgtccagtg cctgcaataa ttcgccaagt
      721 tctttgttac ttaagacacg ttcgcttata tccggttttt tcccaacgtc cgccacgttc
      781 agatcgtcca gaacgttgct gattgcatag cgccgcctac ggcaaaactt aagcgcctgt
      841 ttgcacgcct gtagcacgaa tccggcagta acaggcgttc gctttgccac ctggtcaaaa
      901 caggccagcc agtgccgtag ctcgcattta tccagcggca tagcaccaat ctgctgtatt
      961 acgtgattat taagtcgcct tttcagggcg atataatcca cgcggttttc ctttacgtaa
     1021 tactcaagcc agtaggtgag cgcatcgcca accgttaccg gctttaacgc ttcctgtacg
     1081 gtgtaattaa gctcatgacg tggatttttc ccctctgcca gccatgcgcg acactgggcg
     1141 gctttttccc tggctgattt caggctcaga tcaggataat ttcccagctt aatgcgttcc
     1201 ggtggtgccc cccttcccgt tccggccctg taagtgaaat accaagtcaa tttccctgat
     1261 gttaaatatt tcacgctcag gtttccgcca tcactataaa acgtgttttt ctccgctggc
     1321 ttaccatgaa gtttccttag caaggtatcg ctcagtttgt tcattgctct gctcacgttt
     1381 taccccctct ttacgaaaaa gtgactacac cagtgactac accgatcgtt gcacaaaggg
     1441 ctacaacgtg aaacaagctg gaacaaggaa aatcacaaat ctgttgataa tcaaaaacat
     1501 aatgcacagc ctgaaacatt atgaaacagc aaaaaacact aaatgataaa atgctgttat
     1561 tcttaacccg ctggtgcaac acatggtccg ctggcgtgtg ccgcgtgtac tggcagtgtc
     1621 aattttgatg accatcatcg tgatggcgat ggtgttgctg ttagcttatc tgggttccgc
     1681 gctcaatgag ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca
     1741 agcgattgaa ccgttgttgc aacgcgtagg gattgatgtc tcagttgacc agctggcgca
     1801 ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa
     1861 tgccatgtca tcaatatttt tattgctgct gacggtgctg tttatgttgc tcgaagtgcc
     1921 acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat
     1981 tcagcgtgcg attgacagtg tgtctcatta tctggtgctg aaaacagcca tcagcatcat
     2041 caccggcctg gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg
     2101 gggattgctg gcctttgcgc ttaattacat cccgaatatt ggttcagttc tcgcggcaat
     2161 cccccctatc gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc
     2221 gggatatctg ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg
     2281 tgggctgggg ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt
     2341 aggaccggtg ggtatgctgc tttccgtgcc gttgacaatt attgtcaaaa ttgcgcttga
     2401 acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg
     2461 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
     2521 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     2581 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     2641 caggcatacc agcaatcgga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     2701 tagcaccaat caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt
     2761 ccagcacgat gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca
     2821 aacgccccgc aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct
     2881 cggtaatttc agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa
     2941 tgatcactga atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt
     3001 gctcgcccac ttcctgatca tcaccagtag aagagccgtc ggtgccgaaa ccacccgcaa
     3061 taacgctgat aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcgcccg
     3121 aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt gctaagcata aagcccgcag
     3181 ccgcagccgc ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac
     3241 caccgatgga ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca
     3301 atgccagcac ttgcaggccg acgctgtccg tgcgaacaaa tacaatcagc aacaggaagg
     3361 aaacgaccag agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg
     3421 aaatcttgcc acacagtttg ccgaacgcca ccaccgaccc cgtaaacgtt accgccccga
     3481 tgaagatacc gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg
     3541 cgtcatgatg cagatagctg ttaaaaccaa ccagcactgc cgccagaccc acgaagctat
     3601 gcaggatcgc caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac
     3661 caattgcccc accaataacc atcgccagca agatccagcc aacattaccc gtatccggtc
     3721 caaaaatggt tgcgattaac gcaatcgcca taccggcgat accgaaattg ttaccctggc
     3781 gagacgtttc atgtttcgaa agcccggcca gactgaagat aaacaggatc gcggcaacaa
     3841 tgtatgcagc tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt
     3901 ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc
     3961 gcgataaaac taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca
     4021 acaataatcc ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcgtgcgat
     4081 acattccaca ccacgtaata accgacaacg caggccagcg cgaacacagt gaagtgtcca
     4141 aggaactctt ttggcgcaac gcttgccatc cagccgaaaa ggatgattgc caacgccatc
     4201 aacgcgtatt tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc
     4261 gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt
     4321 tcgcccgcac ggatcacggt cacgccacga atcaccacat catcaaaatc cacagtgatg
     4381 ttgccgtctt tctctttgca caacagtttc agcagattaa cgaggttagt gccgtaaagc
     4441 tgtgaggatt gcgtcggcag acggcccgga agatcggtgt aaccaattac tttgacacca
     4501 ttttccgtag tgaagatttc acccggcacg gtgtattcac agttgccgcc gttttgggct
     4561 gccaggtcga caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc
     4621 ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc
     4681 tgggcggcaa agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag
     4741 ccatcgccgc tgcccgcttc ctctttaaaa tccagctcga ggaattctgc gcccatactt
     4801 tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg
     4861 tttgctgcgc caatggcggc cagacctgcg acaccggcac caatcaccat cacttttgcc
     4921 ggtggcactt tcccggccgc agtgatttgt ccggtaaaga agcgcccaaa ttcatgtgca
     4981 gcttcaacaa tggcgcgata accggcgata ttcgccatcg agcttagtgc gtccagcgat
     5041 tgtgcgcgtg agatacgcgg cacagagtcc atcgccatca cggtcacgtt acgttccgca
     5101 agtttttgca ttaattccgg attctgcgca ggccagataa aactcaccaa cgttgtccca
     5161 ggattcagta acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac
     5221 tgccagacgc tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca
     5281 aaacttgcca gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc
     5341 tgttccactg tttttggtgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt
     5401 atgccaattc gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca
     5461 ggagccacac aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa
     5521 caaacgccct ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt
     5581 cggcgtgaac tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa
     5641 cgccggataa agactgataa ttgtcttcgg cggccgggta aaacgagaca tcgccccggc
     5701 acaaatcact acttaacatt aaattaactt atacaattca gttgcttcag tagtattgat
     5761 ggtgatacgg ctgtttttta agcatagacg gtcatttgag cagaattaaa attggtttaa
     5821 ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac
     5881 gtttctcgtt aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg
     5941 aagcttaaga acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac
     6001 gcagcaacag aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg
     6061 gttaccggtc gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat
     6121 aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac
     6181 tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat
     6241 cgcgtaatta acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt
     6301 gacacggtca ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc
     6361 aaagcggcaa aagcgaaagg tgcctactct ttctacattg ttcgtcaaat cgatgccaac
     6421 cagggcggta accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc
     6481 atccagagcc cggatgtaat cccggcagat tccgaagcag gacgtgcagc tctggctgcc
     6541 ggtggcgaag ccgcgaagaa agttgagatc ccgggtgttg cgactaccgc atcaccaagt
     6601 tctgaagtcg gtcgcttctt tgaaacccag tcatcaaaag gcgggcgtta caccgtcacg
     6661 ctcccggatg gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg
     6721 ttcgacagca tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt
     6781 gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa
     6841 cgtggtaata acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat
     6901 aaggcggctt gatgccgcct ttcgcatttt tagtcataaa tttccctgcg accattgcat
     6961 gcagacacat ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt
     7021 tgccctaata tttattcttc ttgtcacgtt ttaatcactg gatatcgatg gaaaagaaac
     7081 tgggactgag cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca
     7141 gtctgccgca aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta
     7201 ttactggcgc tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgcc
     7261 ccgaacttga cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct
     7321 tttgttccgc atggggatac tggctgtgcg cggtcatcgc caacgtctcc tatctggtga
     7381 tcgttttttc cgcgttaagc ttttttacgg acacgccgga attgcgcctg tttggcgatg
     7441 gtaatacctg gcaatcgatc gtcggtgcat cggctttatt gtggattgtt cactttttga
     7501 tcctgcgcgg ggtgcaaacc gctgccagca ttaaccttgt ggcgacactg gcaaaattgt
     7561 tgccgttggg tctgtttgtt gtgctggcga taatgatgtt caaactggat accttcaagc
     7621 tcgacttcac cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc
     7681 tgatcaccct gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc
     7741 gtaataagcg tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt
     7801 atttgctggt aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc
     7861 gtaacccgtc aatggccggt ctgatggtgg aaatgatggg accgtggggc gaaatcatca
     7921 tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcag
     7981 cagaagttcc gttccttgca gccactcata aagcattccc gcgcattttt gcacgccaga
     8041 acgcacaagc tgcgccatcc gcttcactgt ggctgaccaa catctgcgtg caaatttgtc
     8101 tggtgctcat ctggctgacc ggttccgatt acaacacatt gctgaccatc gcctcagaaa
     8161 tgattctggt gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac
     8221 tgcataaggc ggtaggtgtc ggtgcctgca tttatggctt atggttactg tacgcctctg
     8281 gtccaatgca cttgctgtta tccgttgttc tgtatgcgcc ggggctactg gttttcctgt
     8341 acgcgcgtaa aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg
     8401 gcatgctgtt aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatccc
     8461 atcgttggat agcaaaggag ataacgatgg gtaaaaccca gtccttgcca atattaatta
     8521 ctggcggagg tcgccgcatc ggcctcgccc tcgcatggca tttcattaat caaaagcaac
     8581 cggtgattgt cagctatcgg acgcactatc ccaccattga tggactgatt aaggcaggtg
     8641 cgcagtgtat tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag
     8701 tactaaaaag cacccatggt ctgcgtgcta ttttgcataa cgccagcgcg tggatggcgg
     8761 aaaaaccggg agcgccactg accgacgtac tggcttgcat gatgcagatc cacgttaata
     8821 ccccatacct gctcaaccat gctctggaaa gattactgcg tgggcacgga cacgccgcca
     8881 gcgatatcat tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catattgcct
     8941 atgctgcaag caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac
     9001 cggaagtgaa agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg
     9061 ccgaatatcg acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag
     9121 aagtgatcga cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtctcc
     9181 cacttgatgg cggtcgtcat ctgcgttaat gcagcttaat ccagcatata atcaacaccc
     9241 atgcgcttat tccccagcac gcaacagagc caacaaaagc agcgggcaag cgcatcattc
     9301 cggtgaaata ccatagggac gccaggtaaa caaaataggg aataatcgac cacatactaa
     9361 aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga
     9421 taagcgcaaa ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg
     9481 ctaaaacacc aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca
     9541 tctttttacc ttaacacttc cataacaagt cattagtaga atacctgatg aaaccttgtt
     9601 tagaaacgat tgatagtaag taaaaacagt gcggtgtatt gtgacgtttt tatatctacc
     9661 gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg tgcactgatt
     9721 gccgcatacc tggcaaaaca tgatatgcag gttaccgtag agccgcgcgg cgaccgggcc
     9781 gaagaaacta ttctgcgaga aaatccggat ttggtgttac tcgacatcat gctaccaggc
     9841 aaggacggca tgaccatttg tcgtgattta cgcgcaaagt ggtccggacc gattgttctt
     9901 ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac
     9961 tatattctca aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt
    10021 cagaatgagc aagccacagt gaccaaaggt attcaggaaa cgtctctgac tccctacaaa
    10081 gccctgcatt tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac
    10141 actgaaatct cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc
    10201 gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga
    10261 ctggatcgta gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc
    10321 gcagaacctt atcgtattaa aaccgtgcgt aacaaaggct atctttttgc gcctcatgcg
    10381 tgggaataag cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc
    10441 cttgtgatgt ctctgctggt cgggctggtg tacaaattta ccgccgaacg cgcaggcaaa
    10501 cagtcgctgg atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag
    10561 atccccccac acgactgggg taaaacgctg aaagagatgg atttaaatct ctctttcgat
    10621 ctgcgtgtcg agccgctgag taaatatcat cttgatgata tttccatgca ccggctgcgt
    10681 ggcggcgaaa ttgtcgctct ggacgatcag tacacttttt tgcaacgtat cccgcgcagc
    10741 cactacgtgc tggcagttgg tcctgttcct tatctttatt acctccatca gatgcgattg
    10801 ctggatatcg ccctgatcgc ttttattgcc atttccctcg cctttccggt gtttatctgg
    10861 atgcgtccgc actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat
    10921 gggcatctca gtgagcgtat ccactttgat gaaggttcga gctttgaacg acttggcgtc
    10981 gcatttaacc agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt
    11041 gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg
    11101 agcgataacc tgagcgccgc cgaatcccag gcgttgaatc gcgatatcag tcaacttgaa
    11161 gctttaattg aagagctgct gacttatgcc cgactcgacc gcccacaaaa tgagcttcat
    11221 cttagcgaac cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta
    11281 acgcccgata aaacggtacg gataaaaacg ctcgcgcaag gccattatgc ggcgttggat
    11341 atgcgcttaa tggagcgcgt gctggataat ttgctcaata acgccctgcg ctactgtcac
    11401 tcaacggttg aaatcagcct gctactgtcg gggaatagag cgacattaat tgttgaggat
    11461 gatggcccag ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc
    11521 gatcccagcc gggatcgctc aaccggcggc tgcgggctgg ggctggccat tgtccactct
    11581 atagcactgg caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgcccgc
    11641 ttctcgttca gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactat
    11701 gcgcacggtg gtcaagtcac caccattgtg ctataaaata aggatgttgt aactaaagtg
    11761 gttaatatta tggcgcgtta cgatctcgta gaccgactca acactacttt tcgccagatg
    11821 gaacaagagc tggctgcatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc
    11881 gtgttctctt tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag
    11941 ataaaacaac atctcggcaa cgacgcgcaa tcgctggcgt tgcgtcattt tcgccattta
    12001 tttattcaac aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg
    12061 ttgtgttacc aggtcgataa cttttcgcaa gcagcgttag tcagtcatat tcagcacatc
    12121 aataaactca agaccacgtt cgagcatatc gtcacggttg aatcagaact cccctccgcg
    12181 gcacgttttg aatgggtgca tcgtcatttg ccggggctga ttacccttaa tgcttaccgc
    12241 acgctcaccg ttctgcacga ccccgccact ttacgttttg gctgggctaa taaacatatc
    12301 attaaaaatt tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca
    12361 cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa aactggagcg agaatatcag
    12421 gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag
    12481 ccgattgccc gcgtctggta caaaggagat caaaaacaag tacaacacgc ctgccctacg
    12541 ccgctgattg cactgattaa tagggataat ggcgcgggcg tgccggacgt tggtgagttg
    12601 ttaaattacg atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt
    12661 ttgatcattc cacggctgca cctgtatgtt gcagattaac ccccggcttt catactgccg
    12721 accatctgtt ctggccgtac ccagcggtca aactcggctt cgctaagata ccccagcgca
    12781 agggccgcag cttttaaggt cagcccttct ttatgcgctt ttttggcgat ctcggcggct
    12841 ttgtcataac caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat
    12901 tgattgattc gctcacgatt cggttcaatg cccactgcgc agtgtttgtt gaaactttcc
    12961 atgccatctg ccagcaagcg caccgattgc aggaagttat ggatcaccat cggacggaag
    13021 acgttcagtt caaagttacc ggaagcgcca cccatgttga tcgccacgtc gttccccatc
    13081 acctgacagc agagcatggt taatgcttca cactgtgtcg ggttcacttt ccccggcatg
    13141 attgagctgc ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca
    13201 gaggccagcc agcggacatc attggcaatt ttcatcagtg acgcagccaa ccctttcaat
    13261 gcgccgtgcg cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggtgcg
    13321 gtaacaaacg gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatattcc
    13381 ggatgggtat ttagtccagt acccaccgct gtaccgccca gagccagttc cgctacgtga
    13441 ggcaggctgt attcgatatg tttgagatta tgctccagca tcgctaccca gccggaaatc
    13501 tcctgcccca gcgttaacgg cgtcgcgtcc tgcaagtggg tacgaccgat tttgacgata
    13561 tcggcaaatg cgcgggattt ttcactcagc gtctgggtca gggttttaag ttgtggaatg
    13621 agttgcttgc gcagcgccag tagtgccgca acgtgcatcg ccgtcggaaa gacatcgttg
    13681 gaactttggc ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg
    13741 ccgccgagta attcactggc ccggttagcc agcacttcat tcatgttcat gttactttgc
    13801 gtgccggagc cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt
    13861 acttcatccg ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct
    13921 tcattaactt ttgacgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc
    13981 attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc
    14041 ttatctgccg ggacatcaat cgctcccatc gaatcttttt cgctgcgtac tgtattcatg
    14101 gcctgctcct cacctgatta attttttctt tctgttttgc tttcgctaag caacttttag
    14161 ctcactcatt atttaccatt tgataacaaa cgtttggtct ttcgtgccat gtaaaaaaac
    14221 cgccccgaag ggcggctctg tttatttcac acagcgggtg cattgtgtga gttgtatctg
    14281 ctggaagaag tcattccctt tatcatccac aaggataaac gccgggaaat cttccacttc
    14341 aattttccag atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat
    14401 acttccctgc gccaatacag ctgccggacc accgatactg ccgaggtaga agccgccgtg
    14461 ttttttacaa gcatccgtca cctgctggct gcgattacct ttcgccagca tgatcatact
    14521 tccgccctgc gcttgcaatt gatcgacata agaatccatc cggccagcgg tcgttgggcc
    14581 aagagaaccg gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg
    14641 atctttaata tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg
    14701 agcaatatca cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag aaacgggata
    14761 ctgcgacaac tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc
    14821 gccttctcct gctttgcgca gctcttccgg gatatatttg cctggattat gttccagttt
    14881 ttcgatccag atcccctgac ggttgatctt cgctttgata ttacggtcag cagaacagga
    14941 gacgcccata ccgaccgggc aggatgcgcc gtgacgtggc aggcgaatca cgcggatgtc
    15001 atgagcgaag tatttaccac caaactgcgc acccagacca agattttgcg cttcgatcag
    15061 caattctttt tccagttcca catcgcggaa cgcctgaccg tgctcattcc cttccgttgg
    15121 cagttcatca tagtacttag cggaagcgag tttcaccgtt ttaagattcg tttctgcaga
    15181 agttccacca ataacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg
    15241 catcttctca accaggtaat ttttcagttt tcccggcgtc agtaacgctt tggtttcctg
    15301 atagagatac gtcttgtttg ccgaaccgcc acctttggcg atacagagga atttgtactc
    15361 gtcgccatca acggcataaa gatcgatctg cgctggcaag ttggtgccgg tattcacctc
    15421 tttatacata tccagcggcg cgttttgcga gtagcgcaga ttatcttcga tataagtgtt
    15481 atagacaccg cgtgccagcg ccgcttcatc accgccaccg gtccatacac gctgcccttt
    15541 tttaccaaca ataatcgcgg tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc
    15601 gatatcggag ttgcgcagga attgcagcgc cacatattta tcattttcgc tggcttccgg
    15661 gtcacgcaga atgtcggcca cctgttgttg gtgagccgga cgcagcataa atgacgcatc
    15721 atgaaacgcc tgacgcgcca acaaagttaa cgcttcgggt gcgactttca aaatctcctg
    15781 cccttcaaat tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc
    15841 ttttttgagt ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact
    15901 tactgcctgg tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa
    15961 caatatccta cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag
    16021 gttacttccc gtaggattct tgctttaata gcgggattaa tttccacatt aaaacaggga
    16081 ttgatcatgc aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg
    16141 ttgactgaac tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg
    16201 ggcgcacatc caaaaagcag ttcacgagtg cagaatgccg ccggagatat cgtttcactg
    16261 cgtgatgtga ttgagagtga taaatcgact ctgctcggag aggccgttgc caaacgcttt
    16321 ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt
    16381 catccaaaca aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtattccg
    16441 atggatgccg ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg
    16501 ctgacgcctt tccttgcgat gaacgcgttt cgtgaatttt ccgagattgt ctccctactc
    16561 cagccagtcg caggtgcaca tccggcgatt gctcactttt tacaacagcc tgatgccgaa
    16621 cgtttaagcg aactgttcgc cagcctgctg aatatgcagg gggaagaaaa atcccgcgcg
    16681 ctggcgattt taaaatcggc ccttgacagc cagcagggtg aaccgtggca aacgattcgt
    16741 ttaatttctg aattttaccc ggatgacagc ggtctgttct ccccgctgtt gctgaatgtg
    16801 gtgaaattga atcctggcga agcgatgttc ctgttcgctg aaacgccgca cgcttacctg
    16861 caaggcgtgg cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg
    16921 cctaaatata ttgatattcc ggaactggtt gccaatgtga agttcgaagc caaaccggct
    16981 aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattcctgtt
    17041 gatgattttg ccttctcgct gcatgacctt agtgataaag aaaccaccat cagccagcag
    17101 agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgc tgtgtaaagg ttctcagcag
    17161 ttacagctta aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc
    17221 aaaggccacg gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt
    17281 aacatctctt gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga
    17341 tttcatttta tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc
    17401 gctaggcgta gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct
    17461 cgaagacatg gtcgcgcagg cgaacgcgca actcaaactg accgctcctg aatccaacct
    17521 ggaagtgagt tatcaaaact atcatcgcgg cgtattcagc agtcagctgc aactgttggt
    17581 aaaacccatt gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa
    17641 cgaatcggtt gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc
    17701 atcgatggca tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga
    17761 tatggcaaaa ggtgaaacgc cttttgagat taactcccgc attggttaca gcggagattc
    17821 cagttccgac atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcatt
    17881 tagcggcggc gagttccagt taaatgcaga cagagacggc aaagctatct ccctttccgg
    17941 ggaggcgcaa agtggtcgga tagacgcggt taacgaatac aaccagaaag tgcagttaac
    18001 ctttaataat ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg
    18061 aaaccaaaaa ctgtcgctgg aaaaaatgac catttcagtg gagggcaaag aactggcact
    18121 gctggaaggc atggagatca gcggtaaatc ggatctggtc aatgacggta aaacggtcaa
    18181 tagccaactg gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagtgg
    18241 caagctgact ttaaaagtcg gccaaattga tggcgaagcc tggcatcagt ttagccagca
    18301 atataacgcg caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact
    18361 ttatcaggag aaagtgacgg aagccttctt tagcgctctg ccgctgatgc tgaaaggcga
    18421 tccggtgatt actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa
    18481 tctgtcgctg ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca
    18541 ggaagtggac cggtcggtta aatctctgga tgcgaaactg accattccgg tggatatggc
    18601 aactgagtta atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa
    18661 actggcgaaa cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac
    18721 cttgcaggac aataccatca ccaccagcct gcaatatgct aacggtcaga taacgttaaa
    18781 cgggcagaaa atgccactgg aagatttcgt gggtatgttt gcgatgccgg cattaaacgt
    18841 tccggctgta cccgctattc cgcagcagta attcacagcc aaaacgccgg agatttttct
    18901 ctccggcgtt ttttcttact tcagcataaa gtcatactga atactcattt tcacatccca
    18961 gtaattcagg cgatcgccca gcgcactgcc atcgcgcttt tcccgccctt tatcggcagc
    19021 aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa
    19081 gatacccgcg ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat
    19141 cggtgcaaat gtcagcgtaa attgcggagt taaacgataa ttgacgccca attgccagga
    19201 ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtgtc
    19261 caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca
    19321 cggcgcattc caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt
    19381 acgacttaaa ccttcaagtt gcgcgtaaaa gccgagcaac tcccctttca acgtataatt
    19441 cgggtcaagc ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg
    19501 taccgatgcc cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga
    19561 acgtgcgcga tgtttatcgt attttcccgt ctggctgtta taaacacctt catcacgaat
    19621 accggttgtt gaatcggtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat
    19681 atttggcgtt aaagcccaat aacctaatgc tccttcagaa acagtccgta atgtacgttg
    19741 cgaactgcca gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc
    19801 gaagcgacct aattttagca ggctattttc actgggtttt atttttactg cccccagggc
    19861 aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc
    19921 taatttataa acataatacg ccccgccttc aacgccaata atattggaat aataaccact
    19981 attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg tgccagcact
    20041 gggcttatca gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc
    20101 agccagttgc gccgcgtaag cagaggtaag gccagaggca gccgtcaggc aaataacggc
    20161 catggccact atttttctca tagttgcacc tttg
//
LOCUS       DAZFRG010000071        20114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFRG010000071 DAZFRG010000000
VERSION     DAZFRG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.071"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            40..564
                     /gene="lpfA"
                     /locus_tag="ACHKUC_002017"
     CDS             40..564
                     /gene="lpfA"
                     /locus_tag="ACHKUC_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFO6618273.1"
                     /translation="MKKVVFALSALALTSGSVFAADSGDGTVKFTGEIVDAACVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPSKPFEIKLEDCDITTLKKAKVSFSGVGDTDKPEL
                     ISVSTEAGAAKGVGIGIYDNSNTLVAMNSGEASTDLKKGQTVLYFTANYVSTLAAVTT
                     GYGNAQVDFNLSYE"
     gene            648..1319
                     /gene="lpfB"
                     /locus_tag="ACHKUC_002018"
     CDS             648..1319
                     /gene="lpfB"
                     /locus_tag="ACHKUC_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO6618274.1"
                     /translation="MALTLWVLISQSALASGVGLSSTRVIYDGNKKEASLTVQNKNKS
                     EEFLIQSWVDDATGSKKTPFIITPPLFKLEPGKNNILRIVNVNPSLPQDRESVYWVNV
                     KAIPSQNEENEGKNVLQIAVRTRIKLFYRPAGLKGDIKSSPGELRFTRNGKQIKIDNP
                     TVFNITFSDVFINGHEIEKSGMVPAKGSLNINLPAGVNSASQIKYNTINDFGSSGEYL
                     TREIN"
     gene            1343..3919
                     /gene="lpfC"
                     /locus_tag="ACHKUC_002019"
     CDS             1343..3919
                     /gene="lpfC"
                     /locus_tag="ACHKUC_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO6618275.1"
                     /translation="MSWMVVSRTYTSLPFSLSVLALTVAGSFSATAGKFNPRFLEDTA
                     GINQHVDLSMYETDHGAQLPGTYRVSLIVNEQKMETRTLEFKAATESQRKEMGEFLIP
                     CLSRTQLADMGVRVDSFSALNLIPAEACVAFNEIIPQATSHFDFSEQKLVMSFPQAAM
                     QQVARGTVPESRWDDGVPALLLDYSFSGSNSSHDTKSYNRYIDENGNHHQVKNETSQT
                     NDSYYLSMRSGLNLGAWRLRNYSNWSYSNGEKQWDNIGTYVTRAIVPLKAQLTLGDTA
                     TPSDIFDSVQMRGALLASDEEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRAFV
                     QPGAFEINDLYPTANSGDLTVIVKEADGSEQRFIQPFSSVAIFQREGHLKYSLSAGEY
                     RAGNYDSANPRFAQFNAMYGLPFGMTTYGGALISKDYNAFALGLGKNFGSIGAISVDI
                     TQAKSTLNNNATDQGQSYRFLYSKSFASGTDFRLLGYKYSTSGFYTFQEATDVRSGAD
                     SDYGRYHKRSEIQGNLTQQLGTYGSVYFNMTQQDYWNDDGKRLSLATGYNGRIGRVNY
                     SIAYSWNKSPEWDENDQLWSFNVSIPFGRAWSNYRVTTDQDGRTTQQLGVNGTLLEDR
                     NLSYNVQEGYSSNGVGNSGNASLAYQGGAGNISVGYSYGKDYQQTNYSLRGGIVAHSE
                     GISLSQPLGETIGIVSAPGARGAKVLNNSGVSVDWQGNAVVPYLSIYRENDVSIRSET
                     LNDSVDMNSAFQTVVPTRGAVVRAHFDTRVGYRVLMTLIRQNEVSVPFGATATLVSDK
                     SEQISGIVGEDGQLYISGMPKKGDVKIVWGKDTSQQCVAKYELPIKKKSSGIISVTTN
                     CQ"
     gene            3935..4990
                     /locus_tag="ACHKUC_002020"
     CDS             3935..4990
                     /locus_tag="ACHKUC_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6618276.1"
                     /translation="MMKISSLFFVGSMLFTANNVMAADWGPCTSTSGVHDYPVNFDID
                     IMDASKNEAGTELKLIYSWTGVGQPFNFICECPDTYKTEKDTLIKVVSNLSTATRKIN
                     SEQYYRLTDELEVMTMIQGPSVMFRVPEANSQIPNLNNSSQDCKGNNLGQFGSNGYLS
                     FYIAKPVQGEVEIPYTLIATMYASKKKNTYRDPLSTVSIAGKLTFTQGCKIDAGTVLD
                     VPFGEYPSSAFKNKKGTIPVGGTEKEINLQFDCENITDGIQVSLRIEGNTSQADERAI
                     DMGNPDIGVLVMDPNGNVLKPNDTNSSVNLNLGSNDSQKHRDATIKLKAAPISTTGNA
                     PAAGQYSGVATIFLDMD"
     gene            4996..5526
                     /gene="lpfE"
                     /locus_tag="ACHKUC_002021"
     CDS             4996..5526
                     /gene="lpfE"
                     /locus_tag="ACHKUC_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO6618277.1"
                     /translation="MWRNSIRCLLPFLALAYFPGAEAKISLGEAGKLTFSLSVVAKGC
                     EFEAADLEVVMGKMMLMKPVTVGRVINEKNFVIELKDCDGISKAKVTMDGLPDTNDNS
                     LFALDAGGATGIALQIVDGKGTKQIPKVAGGTAIEWPVNGTTTQLNYKASYVVVNANA
                     TSGHANAMVNFSVEYE"
     gene            5790..7481
                     /gene="eptB"
                     /locus_tag="ACHKUC_002022"
     CDS             5790..7481
                     /gene="eptB"
                     /locus_tag="ACHKUC_002022"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO6618278.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEASAN"
     gene            7570..7646
                     /locus_tag="ACHKUC_002023"
     tRNA            7570..7646
                     /locus_tag="ACHKUC_002023"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7604..7606,aa:Pro,seq:cgg)
     gene            8390..9997
                     /gene="dppA"
                     /locus_tag="ACHKUC_002024"
     CDS             8390..9997
                     /gene="dppA"
                     /locus_tag="ACHKUC_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO6618279.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10304..11323
                     /gene="dppB"
                     /locus_tag="ACHKUC_002025"
     CDS             10304..11323
                     /gene="dppB"
                     /locus_tag="ACHKUC_002025"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO6618280.1"
                     /translation="MLQFILRRLGLVIPTFIGITLFTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11333..12235
                     /gene="dppC"
                     /locus_tag="ACHKUC_002026"
     CDS             11333..12235
                     /gene="dppC"
                     /locus_tag="ACHKUC_002026"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO6618281.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12246..13229
                     /gene="dppD"
                     /locus_tag="ACHKUC_002027"
     CDS             12246..13229
                     /gene="dppD"
                     /locus_tag="ACHKUC_002027"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO6618282.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFQAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13226..14230
                     /gene="dppF"
                     /locus_tag="ACHKUC_002028"
     CDS             13226..14230
                     /gene="dppF"
                     /locus_tag="ACHKUC_002028"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO6618283.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRQEKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14260..15531)
                     /gene="yhjV"
                     /locus_tag="ACHKUC_002029"
     CDS             complement(14260..15531)
                     /gene="yhjV"
                     /locus_tag="ACHKUC_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO6618284.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSLVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            16007..16114
                     /gene="ldrD"
                     /locus_tag="ACHKUC_002030"
     CDS             16007..16114
                     /gene="ldrD"
                     /locus_tag="ACHKUC_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6618285.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16201..17880)
                     /gene="bcsG"
                     /locus_tag="ACHKUC_002031"
     CDS             complement(16201..17880)
                     /gene="bcsG"
                     /locus_tag="ACHKUC_002031"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO6618286.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFYELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17877..18068)
                     /gene="bcsF"
                     /locus_tag="ACHKUC_002032"
     CDS             complement(17877..18068)
                     /gene="bcsF"
                     /locus_tag="ACHKUC_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO6618287.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18065..19636)
                     /gene="bcsE"
                     /locus_tag="ACHKUC_002033"
     CDS             complement(18065..19636)
                     /gene="bcsE"
                     /locus_tag="ACHKUC_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO6618288.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNTPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            19909..20097
                     /gene="bcsR"
                     /locus_tag="ACHKUC_002034"
     CDS             19909..20097
                     /gene="bcsR"
                     /locus_tag="ACHKUC_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO6618289.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5254 a         4681 c         5152 g         5027 t
ORIGIN      
        1 caacattaag catatttatc tatataaatg gagttattca tgaaaaaggt tgtttttgcc
       61 ttgtcagcac tggcgttaac ttctggttcc gtatttgcag cggattccgg tgatggcact
      121 gtgaaattta caggtgaaat cgtcgatgca gcttgcgtac tgtcagttga ttctcagaat
      181 caggaagttg tactgggtca ggtgcaaaaa tctgtatttg ctgcagtcgg tgataaatca
      241 ccatcaaaac ctttcgaaat caagctggaa gattgtgata tcaccacttt gaaaaaggcg
      301 aaagtcagct ttagtggcgt tggtgatacc gataaacctg aacttatctc tgttagcacc
      361 gaagcaggcg cggcgaaagg cgtaggtatc ggtatttatg ataacagcaa caccctggtt
      421 gcgatgaata gtggtgaagc cagcactgac ctgaaaaaag gtcagacagt tctttatttc
      481 accgcaaact acgtttctac tttagctgct gtaactactg gttatggtaa tgctcaggtt
      541 gatttcaatc tgtcttacga gtaatagtta gtttcacaat tggcgaagat gaattctccg
      601 ccaattgtgt tcaacgttca tggatagaat gatgaaaaaa aaaattcatt gcgttaactc
      661 tttgggtgtt aatttcgcag tctgcactag cgagtggggt tgggcttagt tctactcggg
      721 tgatttatga cggtaataag aaagaagcat cgctaaccgt acaaaacaaa aataaatcag
      781 aagaatttct tattcagtca tgggttgatg atgcaacggg tagtaaaaaa acaccattca
      841 tcattactcc gccattattc aaacttgagc caggaaaaaa caacatctta cgtattgtaa
      901 atgtgaatcc gtccttacct caggatcgtg aatctgttta ctgggtaaac gtcaaagcga
      961 ttccttctca aaatgaagaa aatgaaggga aaaatgtctt gcagattgca gttcgtactc
     1021 gcatcaagct tttttatcgc ccagcaggtc taaaagggga tattaaatct tcgccaggag
     1081 aactacgttt tacgcgaaac ggtaaacaga taaaaattga caatccaact gtttttaaca
     1141 tcacgttcag tgatgtattt attaacggac acgaaattga aaagtccggt atggtgccag
     1201 caaaagggtc tttgaatatc aatttgcctg caggtgtgaa cagtgcttcg caaataaaat
     1261 ataatacaat taatgatttt ggctcttcag gtgaatatct gactcgcgaa attaattaat
     1321 agttttcagg ggaaccaact taatgtcatg gatggtcgtt tcgcgcactt acacttcttt
     1381 gcccttttct ttatctgtat tggcgcttac ggttgcaggc tctttttctg cgacagctgg
     1441 aaaatttaat ccgcgttttc tggaagatac cgcagggatc aaccagcatg ttgacctttc
     1501 catgtacgaa actgaccatg gcgcgcagtt gcctggaaca tatcgcgttt ctttaatcgt
     1561 caatgaacaa aaaatggaaa cgcgcactct ggagtttaag gcggctacag aatcccaacg
     1621 aaaggagatg ggggagtttc tcataccttg tttaagccgg acacaactgg ccgatatggg
     1681 cgttcgtgta gacagttttt cagcacttaa ccttatacca gcagaagcct gtgtggcgtt
     1741 taatgaaatt atccctcagg cgaccagtca ttttgatttt agcgaacaaa agctggtgat
     1801 gagttttcct caggcggcaa tgcagcaggt tgctcgcggt acagtacctg aatctcgctg
     1861 ggacgatggt gtgcccgcgt tgcttcttga ttacagtttc agcggtagta acagttctca
     1921 tgatacaaaa agttataatc gctacattga tgaaaatggt aatcatcacc aggttaaaaa
     1981 cgaaactagc cagactaacg atagctacta cttaagtatg cgaagcggcc ttaaccttgg
     2041 ggcatggcgt ctgcgaaatt acagtaactg gagctatagt aacggcgaga agcagtggga
     2101 caatattggc acctatgtaa cacgagccat tgttccgctg aaagcgcaac tgacattggg
     2161 tgatacagca acaccttcag atatttttga cagtgtgcag atgcgcggag cattactggc
     2221 ctctgatgaa gaaatgttgc cagacagcca acgtggtttt gctccggtag ttcgagggat
     2281 agcgaaaagt aacgcggaag tcagtattga acaaaatggt tacgtgattt atcgagcatt
     2341 tgttcagcca ggtgcgtttg aaattaacga tttatacccc acagcgaaca gcggtgattt
     2401 aaccgtaatt gttaaagagg ctgatggtag tgaacaaaga tttattcagc cattctcttc
     2461 agtcgctatt ttccaacgtg aagggcatct caaatatagc ctttctgctg gtgaataccg
     2521 tgctggtaat tacgatagcg ccaacccacg ctttgctcag tttaatgcga tgtatggttt
     2581 accgtttggt atgaccacct atggtggcgc attaatttct aaggattaca acgcatttgc
     2641 tctgggattg ggaaaaaact ttggctctat tggtgcaata tctgttgata ttactcaggc
     2701 aaaaagtaca ttaaataata atgcaaccga tcagggacaa tcgtaccgct ttctttattc
     2761 aaaaagcttt gccagcggca cagatttccg tttattgggt tacaagtatt ctaccagtgg
     2821 tttttatacc ttccaggaag caacagatgt gcgtagcggt gctgacagcg attacggacg
     2881 ttaccataag cgtagtgaaa ttcagggtaa tttgacacag caattgggca cctatgggtc
     2941 ggtttatttc aatatgaccc agcaggatta ctggaatgat gacggtaagc gcctttcttt
     3001 ggcaaccggg tacaacggtc gtattgggcg tgttaattac agtattgctt acagttggaa
     3061 taaaagccct gaatgggatg aaaacgacca gttatggtca tttaatgttt ccattccgtt
     3121 tggtcgggca tggagtaact accgcgtgac aaccgatcag gatggtcgta cgacacaaca
     3181 gcttggcgtc aatggcactc tgttagaaga tcgcaatctt agctataacg ttcaggaagg
     3241 gtattccagt aatggtgttg gtaatagcgg taatgccagt ttggcgtacc agggcggcgc
     3301 cggtaatatc agcgtgggtt acagctatgg taaagactac cagcagacta actacagcct
     3361 gcgtggtggc attgtagcgc acagtgaagg gatctcgctt tcccaacctt tgggtgaaac
     3421 cattggtata gtttcagcac caggtgcccg gggggcgaaa gtattaaata atagtggtgt
     3481 gagtgttgac tggcaaggta atgcggtcgt tccttatctg agcatctacc gtgaaaatga
     3541 tgtgtccatt cgcagtgaaa cgttgaatga cagcgttgat atgaacagcg cattccagac
     3601 agttgtgcca acgcgaggag ctgtcgtacg ggcgcatttc gatacccgtg tgggttatcg
     3661 agttcttatg acattaattc ggcaaaatga agtgagcgtc ccctttggtg caactgcaac
     3721 gctggtttca gataaaagtg aacagattag cggtattgtt ggtgaagatg ggcagttata
     3781 tatcagtggg atgcctaaaa aaggggatgt gaagatcgta tggggtaaag atacttcaca
     3841 acaatgcgtt gcgaaatatg aactgccgat taagaaaaaa tcgtcaggta ttatttctgt
     3901 aacaacaaat tgccaatgat cagggataga taatatgatg aaaatttcaa gtctgttttt
     3961 tgttggaagc atgttgttta cggccaataa tgttatggct gcagattggg gaccctgtac
     4021 atcgacttca ggtgttcatg attacccagt caattttgat attgatatta tggatgccag
     4081 caaaaatgaa gcgggtactg aattaaaatt aatttactca tggactggtg ttgggcagcc
     4141 ttttaatttt atttgtgagt gtccggatac ttataaaacg gaaaaggata cactgattaa
     4201 agtcgtgagt aacttatcta cggcaacgcg aaaaataaac agtgagcaat actaccggtt
     4261 aaccgatgag ctggaagtaa tgaccatgat tcaaggtcca agcgtgatgt tccgcgttcc
     4321 ggaggccaat agtcagatcc ctaatttaaa taactcttct caagactgta aaggtaataa
     4381 tctggggcaa tttggcagta atggctatct gtcattttat atagcaaagc ctgttcaggg
     4441 tgaggtggaa attccctata cgcttattgc aacaatgtat gcctcaaaga aaaaaaatac
     4501 atacagggat ccactttcta cggtcagtat tgcaggaaaa ttgaccttta cacaaggctg
     4561 taaaattgac gccggtacgg tattggatgt ccccttcggc gagtacccat cttcggcgtt
     4621 taaaaataaa aaaggaacca ttccagttgg agggactgaa aaagagatta acctccagtt
     4681 cgattgtgaa aatattaccg atggcattca ggtatctcta aggattgaag gaaacacaag
     4741 tcaggctgac gagcgtgcca tcgatatggg taacccagac attggtgtgt tggttatgga
     4801 tccaaacggc aatgtcttaa agcctaacga taccaacagc agtgttaact taaacctcgg
     4861 ttcgaacgat tcccaaaaac atcgcgatgc aacaattaaa ctgaaagcag caccaatcag
     4921 cacgaccgga aatgctccag cggcagggca atatagtggg gttgcaacaa tctttctgga
     4981 tatggattag tcatcatgtg gagaaatagt atacgttgcc tgttaccctt tttagctctg
     5041 gcatattttc ccggcgctga agctaaaata agcctcggtg aagcaggaaa actgacgttt
     5101 tctctttcag tcgtggccaa agggtgtgag tttgaagccg ctgatttaga agttgtcatg
     5161 gggaaaatga tgttaatgaa gccagtcacg gtcggaagag tcataaacga aaaaaatttc
     5221 gtaatcgaat taaaagattg tgacggaata tcaaaagcta aagtaacaat ggacggcctt
     5281 cctgacacca atgataactc tctttttgct ctggatgccg gaggcgcaac gggaatagcc
     5341 ctgcaaattg ttgacggcaa gggcacaaaa cagattccga aagtcgctgg tggaacggcg
     5401 atagagtggc cagtaaatgg aacaacaacc caactgaact acaaagcgag ttatgtcgtt
     5461 gtaaacgcca atgcaacgtc tggacatgca aatgccatgg tcaatttctc cgtggagtat
     5521 gagtaaccag tttcggactt ttggcagaaa aagaggagat tactcctctt ttttacaaga
     5581 acagttaatt attggtctgc attctgactt gctttttgta aatatctgta gctaaaactc
     5641 acttcattgc cacttttcct tctctctgtg gtctacttaa cgacatgagc agaggatagc
     5701 gttcactact ccggcatttt atcctgatac tcccactgta aaaacggagt gctggatatt
     5761 ttttcccctt taccccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga
     5821 agctgagctt tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttacc
     5881 gccgcttcgg cagctatgcg cacgatttta ccgtctggaa aggtatttct gctgttgttg
     5941 aactggccgc caccgtactg gtgaccttct ttttactgcg tcttctttcg ctgtttggcc
     6001 gccgcagctg gcgtattctg gcatcgctgg tggtgctctt ttccgcaggt gccagctatt
     6061 acatgacctt ccttaatgta gtcattggtt atggcatcat cgcatccgtc atgaccaccg
     6121 atatcgacct gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtaa
     6181 gcgcgttgcc tcttatcctt atctggaata accgctgtcg ttacaccttg ctccgacaac
     6241 tgcgaacccc agggcaacgt attcgcagcc tggcggtcgt cgtactggca ggtattatgg
     6301 tttgggcacc gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgacaggtg
     6361 ttgatttgcc gagttatggc ggcgtcgtcg cgaactctta tctgccatca aactggcttt
     6421 cggcattagg tctttatgcc tgggcgcggg tcgatgaatc ttccgataac aattctctcc
     6481 ttaaccctgc gaagaagttt acgtaccagg ctccacaaaa cgttgatgat acatatgtcg
     6541 ttttcattat tggtgaaacc acgcgctggg atcatatggg cattttcggc tatgaacgca
     6601 ataccacacc gaagctggct caggagaaaa atctggcggc gttccgtggt tactcctgtg
     6661 atactgcaac caaactctca ctgcgttgca tgtttgtacg tcagggtgga gcagaagata
     6721 atccgcagcg cacattaaaa gaacagaaca ttttcgcggt attgaagcag ttaggattca
     6781 gttctgatct ctacgctatg caaagcgaaa tgtggttcta cagcaatacg atggcggaca
     6841 acattgctta tcgtgagcag attggtgcgg agccacgtaa ccgtggcaaa ccggtagatg
     6901 atatgttgct ggtagacgaa atgcagcaat cgctggggcg caacccggat ggtaagcatc
     6961 tgatcattct gcataccaaa ggctcacact ttaactacac ccagcgttac ccgcgcagct
     7021 ttgcgcagtg gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga
     7081 tcaactcata cgacaactcg gtgacctatg tggatcactt tatttccagc gtgattgatc
     7141 aggttcgcga taagaaagcg attgtgttct atgcagctga ccacggtgag tcaattaatg
     7201 aacgcgagca cctgcacggc acgccgcgtg aactggcacc gccggaacag ttccgtgtgc
     7261 cgatgatggt ctggatgtcg gataaatatc tggaaaatcc ggtcaatgcg caggcatttg
     7321 cgcagctgaa aaaagaagcc gacatgaaag tgccacgccg tcatgtagag ctgtacgaca
     7381 ccatcatggg ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact
     7441 ggtgtcacat cccgcaggca aaagaggcgt cggctaacta ggcctgctga ctttctcgcc
     7501 gatcaaaagg cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg
     7561 ccccgcattc ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg
     7621 aggttcgaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     7681 tgcatctgta gctcatgagg aagagaacct ccgggggcgg ggaggttcga ctcgacgtag
     7741 gcctgataag acgcgccagc gtcgcatcag gcaaatgtac cggcccattg tcggatgcgg
     7801 cgcgaccgcc ttatccgatc tacacaccac acatttccct gccaaatttg cgccccatat
     7861 ctcactatcc attaacggat ttgtgacaga aaccattgtc tttttttata tttgcttaaa
     7921 tctttttttg cgttacttat ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca
     7981 tggtattaat cgctcagata tccaccttta cctcagattt ttttcattaa atgcataaaa
     8041 cttaatcacc aaatgttgct aaatattaag ctgattgttc tggttgtgtt ggcgtagcac
     8101 aaatttctct gctgcaaata gctgtttgaa gtttttttaa tctttgtttg tggattctca
     8161 ccgttggtgt aaatcccggt tggctgtatt gacgttttca cattctgttg acagattgta
     8221 ggtcacgagg ggcattttat ggaggatccg cactgttaca ctgatgttaa ttagtacggc
     8281 atccccacct cataacgttg acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca
     8341 acccactgca aagggttaaa acaacaaaca tcacaattgg agcagaataa tgcgtatttc
     8401 cttgaaaaag tcagggatgc tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc
     8461 aagtgttcag gctaaaactc tggtttattg ctcagaagga tctccggaag ggtttaaccc
     8521 gcagctgttt acctccggca ccacctatga cgcctcttcc gtaccgcttt ataaccgtct
     8581 ggttgaattt aaaatcggca ccactgaagt gatcccgggc ctcgctgaaa agtgggaagt
     8641 cagcgaagac ggtaaaacct ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa
     8701 taaagaattc aaaccgacgc gcgaactgaa tgccgatgac gtagtgttct cgttcgatcg
     8761 tcagaaaaac gcgcaaaacc cgtaccataa agtctctggc ggcagctacg aatacttcga
     8821 aggcatgggc ttaccagagc tgatcagcga agtgaaaaag gtggacgata acaccgttca
     8881 gtttgtgctg actcgcccgg aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc
     8941 tattctgtca aaagaatatg ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga
     9001 cctcaaccca atcggtaccg gcccgttcca gttacagcag taccagaaag attcccgtat
     9061 tcgctataaa gcgtttgatg gctactgggg caccaaaccg aagatcgata cgctggtctt
     9121 ctctattact cctgacgctt ccgtgcgtta cgcgaaattg cagaagaacg aatgccaggt
     9181 gatgccgtac ccgaacccgg cagatatcgc ccgcatgaag caggataaat ccatcaacct
     9241 gatggaaatg ccggggctga acgtcggtta tctctcgtat aacgtgcaga aaaaaccact
     9301 ggatgacgtg aaagttcgcc aggctctgac ctacgcggtg aacaaagacg ccatcatcaa
     9361 agcggtttat cagggcgcgg gcgtatcagc gaaaaacctg atcccgccaa ccatgtgggg
     9421 ctataacgac gacgttcagg actacactta cgatcctgaa aaagcgaaag ccttgctgaa
     9481 agaagcgggt ctggaaaaag gtttctccat cgacctgtgg gcaatgccgg tacaacgtcc
     9541 gtataacccg aatgctcgcc gcatggcgga gatgattcag gcagactggg cgaaagtggg
     9601 cgtgcaggcc aaaatcgtca cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg
     9661 cgagcatcag acggtaatga tgggctggac tggcgataac ggggatccgg ataacttctt
     9721 cgccaccctg ttcagctgcg ccgcctctga acaaggctcc aactactcaa aatggtgcta
     9781 caaaccgttt gaagatctga ttcaaccggc gcgtgctacc gacgaccaca ataaacgtgt
     9841 tgaactgtac aaacaagcac aggtcgtgat gcacgatcag gctccggcac tgatcatcgc
     9901 tcactccacc gtgtttgaac cggtacgcaa agaagttaaa ggctatgtgg ttgatccatt
     9961 aggcaaacat cacttcgaaa atgtctctat cgaataatta aagccatgca agactgatgg
    10021 caaaggcaaa actgcctgat gcgctccgct tatcaggcct acgaaaactc tgcaatgtat
    10081 tgagtttgca cgattttgta ggtcggataa ggcgtttgcg ccgcatccgg cataaacaaa
    10141 gcgctctttg tcaacaatct gcatacccgg tggcgctgtg cctctgcgtg cagcgccatc
    10201 cggcagcaat acttcattcc ctgttccaca agggcgggaa ttgatttgtg agcaatacag
    10261 acacgcagtt ccaggctgcg ggtcactata gagaatccgg gttatgttgc agtttattct
    10321 ccgacgtttg ggactcgtca tccccacgtt tatcggtatt acccttttca catttgcctt
    10381 tgtccacatg atcccgggcg atccggtgat gatcatggcg ggcgaacgtg ggatctcccc
    10441 agagcgtcac gcgcaattgc tggctgaact cggcttagat aaaccgatgt ggcagcagta
    10501 tctccattac atttggggcg taatgcacgg tgatttaggc atttccatga aaagccgaat
    10561 tccggtatgg gaagagttcg tgccgcgctt tcaggccacg ctggaacttg gcgtctgcgc
    10621 gatgattttt gcgaccgcag tcgggattcc ggttggtgtg ctggctgcgg ttaaacgtgg
    10681 ttccattttc gatcacacag cggttggcct ggcgctgacc ggctactcga tgccaatttt
    10741 ctggtggggc atgatgctga tcatgctggt ttcggtgcac tggaacctga cgcctgtctc
    10801 cggtcgtgtg agcgacatgg tgttcctcga tgattccaat ccgttaaccg gttttatgct
    10861 gatcgacacc gccatctggg gtgaagacgg taactttatc gatgccgtcg cccatatgat
    10921 cctgcccgcc attgtgctgg gtaccatccc gctggcggtc attgtgcgta tgacgcgctc
    10981 ctcgatgctg gaagtgctgg gcgaagatta tatccgcacc gcgcgtgcca aaggtcttac
    11041 ccggatgcgg gtgattatcg tccatgcgct gcgtaacgcg atgctgccgg tggtgaccgt
    11101 tatcggcctg caggtgggaa cattgctggc gggggcgatt ctgactgaaa ccatcttctc
    11161 gtggcccggt ctggggcgct ggttgattga cgcactgcaa cgccgcgatt atccggtagt
    11221 gcagggcggc gtattgctgg tggcgacgat gattatcctc gtcaacctgc tggtcgatct
    11281 gctgtacggc gtggtgaacc cgcgtattcg tcataagaag taaggggaca tcatgtcaca
    11341 ggttactgaa aataaagtga ttagcgcacc ggtgccgatg accccgttac aggagttctg
    11401 gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg gtttacgtcg tcatcgtgct
    11461 gtttatcgcg atctttgcca actggattgc accctataac ccagcggaac agttccgcga
    11521 tgcactgctc gccccgccag cctggcagga aggcggcagc atggcgcact tgctgggcac
    11581 tgatgacgtg gggcgtgatg tgctgtcgcg gctgatgtac ggcgcgcgct tgtcgctgct
    11641 ggttggctgt ctggtagtgg tgttatcgct gattatgggc gttattctcg gcctgatcgc
    11701 tggttacttt ggcggtctgg tcgataacat catcatgcgc gtggtcgata tcatgctggc
    11761 gctgccaagt ctactgctgg cactggtgct ggtggcgatt ttcggcccgt cgattggtaa
    11821 cgccgcgctg gcgctaacct tcgttgcgct gccgcattat gtgcgcttaa cccgcgccgc
    11881 cgtgctggtg gaagttaacc gcgattacgt caccgcgtct cgcgtggcgg gtgccggggc
    11941 aatgcgccag atgtttatta acatcttccc gaactgcctt gcgccgctga ttgttcaggc
    12001 gtcgctcggt ttctctaacg ccattctcga tatggctgct cttggtttcc tcggcatggg
    12061 cgcgcagccg ccaacgccgg agtggggcac catgctctct gacgtgttgc agttcgcgca
    12121 aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc ctgctgacgg tgctggcatt
    12181 taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc aaactgaagc agtaagaggt
    12241 tcgagatggc gttattaaat gtagataaat tatcggtgca tttcggcgac gaaagcgcac
    12301 cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca gggtgaagtg gtcgggattg
    12361 tgggtgagtc cggctctggt aaatcggtca gttcgctggc aattatgggg ctgattgatt
    12421 atccgggccg cgtaatggcg gaaaagctgg agtttaacgg tcaggatttg cagcgtatct
    12481 cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc aatgatcttc caggacccga
    12541 tgaccagcct taacccgtgc tacaccgtgg gtttccagat tatggaagcg attaaggtgc
    12601 atcagggcgg caacaaaagt acccgccgtc agcgagcgat tgacctgctg aatctggtcg
    12661 gtattcccga tccggcatcg cgtctggatg tttacccgca tcagctttcc ggcggcatga
    12721 gccagcgcgt gatgatcgcc atggcgattg cctgtcgacc aaaactgctg attgccgatg
    12781 aaccgaccac tgcgctggac gtgaccatcc aggcgcaaat catcgaactg ctgctggagt
    12841 tgcagcagaa agagaacatg gcgctggtgt tgatcactca tgacctggcg ctggtggcgg
    12901 aagcggcaca taaaatcatc gtgatgtatg caggccaggt agtagaaacg ggcgatgcgc
    12961 acgctatctt ccaggcgccg cgtcacccgt atactcaggc attgctgcgt gcgctgccgg
    13021 aatttgctca ggacaaagaa cgtctggcgt cgttgccagg tgtcgttccc ggcaagtacg
    13081 accgcccgaa cggctgcctg cttaacccgc gctgccccta cgccactgac aaatgccgtg
    13141 ctgaagaacc ggcgctgaat atgctcgctg acgggcgtca gtccaaatgc cattacccac
    13201 ttgatgatgc cgggaggccg acactatgag tacgcatgag gccacctcgc aacaaccgct
    13261 gttgcaggct atcgacctga aaaaacatta tccggtgaag aaaggcatgt tcgcgccgga
    13321 acgtctggtg aaggcgctgg atggcgtttc gtttaacctt gaacgtggca aaacgctggc
    13381 agtagtaggt gaatctggct gcggtaaatc gaccctcggt cggttgctga cgatgattga
    13441 aacgcccacc ggcggcgaac tgtattacca ggggcaggat ctgctcaagc acgatccgca
    13501 ggcgcagaag ctacgtcggc agaaaatcca gattgtcttc cagaaccctt acggttcgct
    13561 gaatccgcgt aaaaaagttg ggcagattct cgaagagccg ctgctgatca acaccagctt
    13621 aagcaaagaa cagcgtcagg aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac
    13681 cgagcattac gaccgctatc cgcatatgtt ctccggcggt cagcgtcagc gtatcgccat
    13741 tgcccgaggt ctgatgctcg acccggatgt ggtgattgcc gatgagccgg tttctgcgct
    13801 ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg gatttgcagc aggaactggg
    13861 gctatcttat gtctttatct cgcacgatct gtcggtggtg gagcacattg ctgatgaagt
    13921 gatggtgatg tacctgggcc gctgcgtgga gaaggggacg aaggaccaaa tcttcaataa
    13981 cccgcgccac ccgtacactc aggcattgct ctccgcgaca ccacgcctga acccggacga
    14041 tcgtcgcgaa cgcatcaaac ttaccggtga actgccaagc ccgctgaatc caccgccagg
    14101 ctgcgccttc aacgcccgct gtcgtcggcg cttcggcccc tgcatccagt tgcagccgca
    14161 gctaaaagac tacggcggtc aactggtagc ctgttttgcc gttgatcagg atgaaaatcc
    14221 gcagcgttaa ctcacaaaac cggggccatg cgctccggtt tactcaatca acttcaacag
    14281 tggcgacaag cataacaaaa tgccgtacag cagaatcatc caggctttaa acccgcgaag
    14341 tttttccagt tgtgagactt tatagatcag gaaaaacgga atgaggcacg acacaatgcc
    14401 atataacggg cttcccaact gaaagaacac cagtaccgat acacgaaacg aaacccaaat
    14461 cgtcaacgta atgacgataa aagtgcagat cgccagagtc agcacgagtg agttaatttt
    14521 cctggtatca ataattcggc ttaacagatt gagaataatg cctttaatgg cctcgtggaa
    14581 accgagataa atgccaaaga atgcagtcag tacggcaaag atattcagta ccgtagaggt
    14641 gatatgaatg atatgcccag ggatcacctg cgcggccagt gccagtgccg agatattttg
    14701 ttcaaaggca gaaacggctt cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa
    14761 caagatcacc gcaatgagcg tgatatagct aatccggtgg gtacgcagcg cgagccgggt
    14821 tgccagtact ttatccgctt cccgtttacg ataggcaata ttcattgggt taagtacctg
    14881 aataaatacc gcggaaaaga agcaaaatgg aatagtaagc aggacatcgc ggaaaaagtc
    14941 ggaggcttgc gggaaggcgg ttatattggc gaaattccag tgcgggatca tcgcaaaacc
    15001 gaacacgaca ataatcccca ctttgaccac caccattggt ccggaaatct taaacagtaa
    15061 tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa atggcgactt tataaaatag
    15121 agattgagaa agatcggcat cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa
    15181 aaccacggag agagagtaga taaaaatccc gtggataatc atcaaaaagt agataacccc
    15241 gaggaaaatt ccccagttct tccccaggta atgactgata atatcggtgt agtcattaca
    15301 ggaatcgctt tcagaaaggg tttttaaata aatgtcctgt actacccagg tggcaggata
    15361 agcaatgatc gctgcggtaa taaataccca aattcccttc aagccaattt gtactggcat
    15421 cagcacggtt ccggcaccaa tcgccatgcc tatgcataat aaaacccagc cgaagtcgta
    15481 gcgtgtaaac ggcaatttct gatcgtgttt cggtagtgtg ttgtgctgca taaagtcctg
    15541 tccagaaaga agtcggcctc gcaaaaatga ataaccccag tttctcttgc gcaacaggtg
    15601 gagaagtcga atacgcagta tgtaaaaaat gaggcaacag attcactggg gaggtcgcga
    15661 ggaggcgcaa ttctacaaaa ttgcgcattt cactactagt ctggagtcag cccggatatg
    15721 agctgcaact attgcaaata aagaaattaa gctggaaggc atcacgcaat gaggaagatt
    15781 gccgggtgct gacggcaaaa aacaatcgta aaatcagcgg caagccggaa aggttccggt
    15841 gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc
    15901 cccgcacgtt gcaggtatgc acctgacaat atcacggggg ctaatcttga acctagacca
    15961 ctcaagaata gccgcgaaac gttgtcatta caacacaggc ggctatatga cgctcgcaga
    16021 actgggcatg gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag
    16081 tatgatcgtg aactggctga ataagcggaa gtaacgtgtc atgcgggcgt caggctgccg
    16141 taatggcaat ttgcgtccgg atcaggccgc aggggtgaaa ctctgcggcc tttttcgttc
    16201 ttattgcggg taaggcaccc agtcgccgcc gttcaggcga acgtacggtt tatcctggta
    16261 ttgaataact actgcatttg agttctcgga gaccggtgct gtttgtggca acccactggt
    16321 gagttttttc cagtcaacat tgtcttcgga gaaaatcttg ccatcaagaa cgcgaaccac
    16381 cagatcggag atagccagga agctgctcgg ttggtcgatg acaatcggag ccccctgatg
    16441 tggtgccttc atgccgaaga atttcacccc aacggggacg tctgtgatag acgggctagg
    16501 gatatcacgt agaccagata cctgcattct gtcgcccttc agcgcgccgc cgtgttccgg
    16561 caccacgacc accatcactt tacgacccga tttctccagt tcagtaaaga aggcgtccag
    16621 ttcataaaag aatttttgcg cccgcgcttt gtaatccgct gttttgctga cgcccggata
    16681 atggttgccg tcatgcagtg gaagcgtgtt gtagaacgtg gcactacggc tgtttttatc
    16741 tttttcggta acgtccagcc aacggttaag cacggcggta tcgtcataaa ccggcgaacc
    16801 atcaaagccc agcaaaataa ccggcagatt tgtttgatcc atcaattcgc tctgcatgcc
    16861 gccattttcg cgaacttctt tcaaaaaacc gccgaactgg ccgttatgtc ccatcatcag
    16921 gtgctgggta aagcccagtt tcgacaggtt atcaaacaga tagcagtcgt tatttgccgg
    16981 ttgatacaga ttagtgtgcg aagtctgccc gcagctggcg cgcagtaaac ggatcgccgc
    17041 cgggccactg taggaggtgg cggagttaaa gttcttgaac tcaatatcga aatgcgacca
    17101 cagtggatgc gacatcaatc cggcggcttc aatatccgac caggaaagcg aacagatatt
    17161 aatcaccagt agttcaaatg gctgagcatc agcgggcagc gaagacggga agatcgattt
    17221 acgtttcgcc tccgcgttat agaaattatt cagccaggcg ttaaggtttg ctgttgttgg
    17281 cggtgcagtt tgtgcgggta tatcacccac taccggtgcg ccacccgtcg ccgcaacggt
    17341 tgccgctgcg ttaccacccg tcgttgttac agtggtcgtc ggttgaccgg ctggccacaa
    17401 ggagaaactt ggtcccgcca gggtaagtac gttcagccat agcagaatgg caaccacaaa
    17461 aacggtaatg cgaatccatt gtgacaggaa taaccaggcc acgaataaaa caaaaatggc
    17521 cccaatcatc tgccagttaa taaagcgtgt gacaagatcg attaaataat cggtactgaa
    17581 ccccgccacc tgcgaaccct ggctcattat gctttccggg ccaggcaacc aggtgtcatg
    17641 ccagaacaaa gcaaagccga tcggcagggc aatccagtgg cgcaagcgat gcaggctgta
    17701 gcgcggaatg ggcatcagca gaaacgcggc aaacaccaga ttgaggagcg gatggaagtt
    17761 aagatatccc gcccacaaca ggccgaactt aaccagaaaa tagaagttcc agccggaaag
    17821 gccgcgccag tattgccaga gggaagaagg catggcggta ttttgcgtaa attgagtcat
    17881 tttttggttg ccctggcttt ttccgtgcgg cgtaacgtcc cggccggttt aacataacga
    17941 ggtttagcaa agaacaaacg taaggtgtcg cgaatgcgtc gcaaagagtg ccgcgccaga
    18001 tagcccagcg ggaaaaatat cagtgcgcaa acgacaataa tttcaatgat atcgctgatg
    18061 gtcatcatga tgagcgctcc acagcatcat ctaacagtcg cattggttct ggtattcgtc
    18121 gccagtggcg accatcgtgc tcggcattga tgaccggttt agaactttgc gctaaaggca
    18181 gcggcatgcc ccattgttct ggggcaagca ggcgcatctg caccagctcg gcactgattt
    18241 gatcatcttc aaaccagacc attcggtttg agaaaatgtc gccagtaggc aatgggaaaa
    18301 tatgattcaa cgcggtatcc agatcgttaa tccgacagaa tgagagaaac agcaccagcc
    18361 gattaccgcc aatggtcatg atatcgccgg tacggttagg gcgacatagc gtcagggctt
    18421 gctcaacgcg gatacccggt accggacgta gggcaaccag aacgccttta ccgtgggcag
    18481 gtaatagagg ggtattcatc atgttgttga cggcattaca gaacacatcc cacttctgga
    18541 aaccacgcag tttgagcggc tgggtcattg acagcaaggt agtgatatct tccggcacat
    18601 agcgactaaa cttctgccct tgcacgcttt cgatcatcgt cagacagcgg gagagtggcg
    18661 cattccacgg gataaccata tttgcaccgc aggccaataa taaacgttca tcggtggcgc
    18721 gcaggctggc ggtattttcc cgtacgagga ttttcatcgc actaccgcgc tggcgacgca
    18781 gagtatgaat gctgcgggcc agtggctcga tttgcgcatt ttgctgtaaa gaaaagacca
    18841 ccgtcgccgc ctgagcggta cgggcttcat tgaacaggac ttcgttattg ttgaacagtt
    18901 gccagtgttc cgatagcggc ggcgcacctt ccagtacagc aacattactc agaatgcgtt
    18961 tttcgtcgct gcgtggttgg atttccgcct cctggtgttg aactaatgtc cagcaaccat
    19021 tttgttgctg aacgctaagc tgctgacggg cgctgacccc tttttcgttg caccagaaag
    19081 caatatccag caaatgttgg tcgccctgaa aacgcaaact ggcaagacca aaaagtgaac
    19141 ggtattcctc aagtaacaat gaaaattgtt tatcgttatt atttccggaa ttaattacca
    19201 aaagcgaaca atggtttagc cggctccatt tattcatttt atccaaccat gagcgaagcc
    19261 gctcggcagg aatgttttgc catgcgttat ttgcgcaaac aagaataaaa aggtaattat
    19321 ggggatcaat agaacactgc aaatcacggg ccaaatagta tagacctttt tcatgattta
    19381 gcattgaaaa taatgttatt tttcccggcc cttgagaatc atctaattga aagattttcg
    19441 ctggatcgct gtccatgcta atgaccgcga cgtgtgcggt ttcagcctgg gatgcaattg
    19501 tttgattcgc cagactgata gcatcttcat ggcgatcgac gttaaaccac cagacgccgc
    19561 ctgctggcat atgtcgcagc tcatcccata atgatgagat accgatagag aatacagggt
    19621 ccacaatgtc cctcatgtca acattttgtc tgattatcag tttactagcg aaagctaaga
    19681 aataaaccta ccattgaaat taaaacttat cagatttagc atgtgaatca atttagcagg
    19741 tcacaagaca tgtgatttta gtcttttcgt ttttaacttt ttctgttgcg taacggaaag
    19801 tcaaaaagtg agcacattcc cgtctcgccg taaacaataa accgacgaaa tgctcacagt
    19861 tagaattgtc tcatgaacgg tacggttatt tcatagggat caagaaaaat gaataacaat
    19921 gaaccagata ctctgcctga tcccgcgata ggctatatct tccagaatga tattttggcg
    19981 ttaaagcagg cattttcact gcctgatatt gattatgccg atatttccca acgcgaacag
    20041 ttggccgcgg cattaaaacg ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg
    20101 attggtgaat ggcc
//
LOCUS       DAZFRG010000072        19897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFRG010000072 DAZFRG010000000
VERSION     DAZFRG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.072"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..1183
                     /gene="chaA"
                     /locus_tag="ACHKUC_002035"
     CDS             83..1183
                     /gene="chaA"
                     /locus_tag="ACHKUC_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO6618290.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1589..1696
                     /gene="ldrA"
                     /locus_tag="ACHKUC_002036"
     CDS             1589..1696
                     /gene="ldrA"
                     /locus_tag="ACHKUC_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6618291.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(1844..2698)
                     /gene="kdsA"
                     /locus_tag="ACHKUC_002037"
     CDS             complement(1844..2698)
                     /gene="kdsA"
                     /locus_tag="ACHKUC_002037"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO6618292.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(2734..3543)
                     /gene="sirB1"
                     /locus_tag="ACHKUC_002038"
     CDS             complement(2734..3543)
                     /gene="sirB1"
                     /locus_tag="ACHKUC_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO6618293.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(3547..3939)
                     /gene="sirB2"
                     /locus_tag="ACHKUC_002039"
     CDS             complement(3547..3939)
                     /gene="sirB2"
                     /locus_tag="ACHKUC_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO6618294.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(3936..4769)
                     /gene="prmC"
                     /locus_tag="ACHKUC_002040"
     CDS             complement(3936..4769)
                     /gene="prmC"
                     /locus_tag="ACHKUC_002040"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6618295.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(4769..5851)
                     /gene="prfA"
                     /locus_tag="ACHKUC_002041"
     CDS             complement(4769..5851)
                     /gene="prfA"
                     /locus_tag="ACHKUC_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO6618296.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(5893..7149)
                     /gene="hemA"
                     /locus_tag="ACHKUC_002042"
     CDS             complement(5893..7149)
                     /gene="hemA"
                     /locus_tag="ACHKUC_002042"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO6618297.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7363..7986
                     /gene="lolB"
                     /locus_tag="ACHKUC_002043"
     CDS             7363..7986
                     /gene="lolB"
                     /locus_tag="ACHKUC_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO6618298.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7986..8837
                     /gene="ispE"
                     /locus_tag="ACHKUC_002044"
     CDS             7986..8837
                     /gene="ispE"
                     /locus_tag="ACHKUC_002044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO6618299.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSMGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     MPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            8988..9935
                     /gene="prs"
                     /locus_tag="ACHKUC_002045"
     CDS             8988..9935
                     /gene="prs"
                     /locus_tag="ACHKUC_002045"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO6618300.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            10060..11739
                     /gene="dauA"
                     /locus_tag="ACHKUC_002046"
     CDS             10060..11739
                     /gene="dauA"
                     /locus_tag="ACHKUC_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO6618301.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(11794..12072)
                     /gene="ychH"
                     /locus_tag="ACHKUC_002047"
     CDS             complement(11794..12072)
                     /gene="ychH"
                     /locus_tag="ACHKUC_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO6618302.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            12350..12934
                     /gene="pth"
                     /locus_tag="ACHKUC_002048"
     CDS             12350..12934
                     /gene="pth"
                     /locus_tag="ACHKUC_002048"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO6618303.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKDKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            13051..14142
                     /gene="ychF"
                     /locus_tag="ACHKUC_002049"
     CDS             13051..14142
                     /gene="ychF"
                     /locus_tag="ACHKUC_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO6618304.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDNDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            14332..14508
                     /locus_tag="ACHKUC_002050"
     CDS             14332..14508
                     /locus_tag="ACHKUC_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618305.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(14545..16464)
                     /gene="dhaR"
                     /locus_tag="ACHKUC_002051"
     CDS             complement(14545..16464)
                     /gene="dhaR"
                     /locus_tag="ACHKUC_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO6618306.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDSENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            16692..17762
                     /gene="dhaK"
                     /locus_tag="ACHKUC_002052"
     CDS             16692..17762
                     /gene="dhaK"
                     /locus_tag="ACHKUC_002052"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO6618307.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            17773..18405
                     /gene="dhaL"
                     /locus_tag="ACHKUC_002053"
     CDS             17773..18405
                     /gene="dhaL"
                     /locus_tag="ACHKUC_002053"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO6618308.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            18416..19834
                     /gene="dhaM"
                     /locus_tag="ACHKUC_002054"
     CDS             18416..19834
                     /gene="dhaM"
                     /locus_tag="ACHKUC_002054"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO6618309.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAIKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         4806 a         5146 c         4987 g         4958 t
ORIGIN      
        1 accgatatcc agacaatttc tgttattttt cctacctatt ttttacattt tcgataaatt
       61 catctgcaaa gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa
      121 ggagacttcg cttattttcc cggttctggc gctggtagtg ctgttcctgt ggggaagcag
      181 ccagacacta ccagtggtca ttgccatcaa tcttcttgcg cttattggta ttttaagtag
      241 cgcctttagt gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg
      301 ttcgcttatt cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat
      361 ggcaaccggc gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat
      421 tgttacaggt gggctggttg gcttttcatt attgttgggc ggacgtaagt ttgccaccca
      481 atatatgaat ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat
      541 cgtactggta tttccaatgg ctctgcctgc ggcgaatttt tcaacaggtc aggcgttact
      601 ggtagcatta atttctgcgg caatgtatgg cgtatttttg ctgatccaga ccaaaacgca
      661 tcaaagtttg tttgtctacg agcacgaaga tgacagtgat gatgacgacc cgcaccacgg
      721 taaaccgtct gcccatagca gcctgtggca tgccatctgg ttgattgtcc atctgattgc
      781 cgttattgca gtcaccaaaa tgaacgccag cccgctggag acattgctcg acagcatgaa
      841 tgcgcctgtc gcctttactg gcttcctggt ggctctgttg attctgtcgc cggaaggttt
      901 aggtgcgtta aaagcagtgt tgaataacca ggtccagcgc gcgatgaatc tgttctttgg
      961 ttcagtgttg gcaaccattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac
     1021 gggtaacgaa ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt
     1081 agtactgtgc catatctcct tctccaccgg acgaactaac gtgctcaatg gcgcagcgca
     1141 tctggcactg tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg
     1201 atgacggaaa tcgggggaga taatataaat tcctgcttat tgtatttctt catactacct
     1261 ggacaggtcg ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac
     1321 agctgttgaa aagccggaat gtactcttca atgcaaacat ttcagggatg aagtgaagat
     1381 gaattgcgac gtagtatcca caccaagccg gcgttatccg gtgaggcgca atgttgcggg
     1441 ggctttatcc ctggtggcat tggttgctgg aaagagaaaa cccccgcacg ttgagaggta
     1501 aaacctgaca acagaacggg ggctaatctt gaaccatcaa caatccaaga atagccgcaa
     1561 aatgttgtca ttacaacaag gcggctatat gacgctcgcg cagtttgcca tgattttctg
     1621 gcacgacctg gcagcaccga tcctggcggg aattattacc gcagcgattg tcggctggtg
     1681 gcgtaaccgg aagtaaggtg aaaacgggcg tcaggctgcc gtgatggcaa tatgcgcccg
     1741 aaaccggccg caggggtaac agcctgcggc cttttttgta tccggcaccc ataaaaaaag
     1801 tcttaacgca gaacgctaag actttatttt tcaaacaaaa agattacttg ctggtatcca
     1861 gttcttcgaa acctttcacc agatcatcaa tcgctttcat ttgcttgagg aacggttcca
     1921 gtttagccag cggcagcgcg gatggaccat cacatttcgc atgttccgga tccggatgcg
     1981 cttcaataaa cagccccgcc agacctaccg ccataccggc tcttgccagc tcagccacct
     2041 gagcacggcg accgccggaa gctgcgccaa atggatcgcg acattgcagt gcgtgggtaa
     2101 cgtcgaaaat caccggcgaa ttaccagaca ctttcttcat gatgctgaag cccagcatat
     2161 caacaaccag gttgtcatag ccgaagttag caccgcgatc gcaaagaatc actttttcgt
     2221 tgccgccttc tttgaattta tcaacgatat tacccatctg tcccgggctg acaaactgcg
     2281 gtttcttgac gttaattacc gcaccggttt tcgccatggc ttcaaccagg tcagtctggc
     2341 gagcaagaaa cgccggcaac tgaatcacat ctacaacatc agcaacgggc tgtgcctggc
     2401 ttggttcgtg aacgtcggtg ataattttca cgccaaaagt ctgcttcaac tcctggaaga
     2461 ttttcatccc ttcttccagg cccggtccac gataagagtg gatggaggag cggttggctt
     2521 tgtcaaaaga ggctttgaac acgtaaggga tacccagttt ctgggtcacg gttacgtagt
     2581 gctcgcaaat gcgcatcgcc agatcgcgag attccagcac gttcataccg ccaaacagta
     2641 cgaacggcag gtcatttgct acgttgatgt cgccaatgct aaccactttt tgtttcatag
     2701 gatcgcctta atcttgagta aaatgtcgat taattaatgc agcacaatat gtttatgcgc
     2761 gatgttattt atttgcgcac ggatcatttc gctgatcggg tcttccggac actgttcaac
     2821 gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc agttgcgcat aaatcaaccc
     2881 gcgatcgcga atttcatagg gatcttccgg gttgaattgt aataaagctt cgctggtgcg
     2941 taacgccagc tccatctgat tttcttccat caacgaagct ttgagtgtat ccagcaattt
     3001 gcggattacc tcaatgttat cagcttcatc aaggtcttca taaaacagtt ccgccgacgg
     3061 gctgatattt ccctttaacc acacatccag catatgttcg cttaacgatt caccgttaaa
     3121 aggattaatc agccaaattt cgccatccgg acattcaatg cgcaatatca gctgcgtagg
     3181 gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg acccataata aaaccgcacc
     3241 taatgatacc gcactgccct gtcgattctt taacacctgg tccagccata atgcatcgga
     3301 aagacgataa acgccgcgtg aggctttaaa cccccattcg ccgtaaaaca gcgcgattaa
     3361 tttttctagc tgctcttcta atggcagaag ctggctaatt tcttccttcg ccagactaac
     3421 gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa tcgcggcgga ttgcttcgca
     3481 agccaggatc atgccttcgc acaatggcgc tttattaaat tcgaaatcag ctaacgatct
     3541 catgacttac cccagtaacg gtacttttgt ggtggcgagt ttaatgatga tgtacagcac
     3601 caccaacgcc agcgggaagg caataatgcg cgcctgctga ctgtgcatac gacgataatc
     3661 gagtgcaata aaacccaaaa cgatataaat gataactcca aacagctttt cagtcagcca
     3721 ctgtgcctgc ccggaaaatg gctggatgtg cgctttagcc atcaacgcaa tgccgcttaa
     3781 caataacagc gtatcgacaa ccggcggcac aatgcgcgtc cagcgagcaa atgcctgtgg
     3841 atgcttctga taacgtagcc agaaacgtaa ggtcaatagc ccaacagaaa gcgcgatact
     3901 aatgagatga acgctaagca acgtagaaaa acttgtcatt gataatagcg gccgagcgtt
     3961 acgcgctcgt tatccccata gtcacggcag gtttcgacgt catgataccc cgcgaagata
     4021 aatgcctgtc gcaccgcttc accctgctgc cagccatgct ccagaagcag aaagccgccg
     4081 gataccagcg cgttacgcga ctgttcgatg atatgcacga tgtctgccat tccactgtct
     4141 gccgcaacca gcgcagtgag cggctcaaag cggacatcgc cttgttgaag atgtgggtcc
     4201 tgctcgtcaa tatacggcgg attgctgaca atcatcgcaa actgctgccc ggctagcgcg
     4261 ctaaaccagt cgctttgcag aatgtggata tttttgatag ccagatgctg ggcattacgt
     4321 tgcgccagag agacagcatc tggcatacga tctacagcgg taatttcgca gtccggacgc
     4381 tcgctggcca gcgccagcgc aatcgccccg gtacccgtcc cgagatcgag aatacggcaa
     4441 ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac actccgtatc cgggcgcgga
     4501 attaaggtcg ctggcgaaac aaataacggc aacgaccaga attctcgcac cccggttaaa
     4561 tgagcaatgg gttcaccatc gcgacgacgc gtcagtagcg catcaagttg ctgacattgt
     4621 tcgtcagtca gctgcgtttc accaaaggcg agaataaaag tacgccctct gccggtaaca
     4681 tgctccagca ggatttcagc atcacgccgc gggctttcgc tcgcctgaag ttggcttatt
     4741 gcttcacgta accagtgttg atattccatt attcctgctc ggacaacgcc gccagttggt
     4801 cggcctgatg ttcctggata atcggttcaa tcagcatatc cagcttacct tccatcactt
     4861 catccaggcg gtagagcgtc aggttgatgc ggtgatcggt aacgcgcccc tgcgggaagt
     4921 tgtaagtacg gttacggtcg ctgcgatcgc cactccccag caggttacga cgggtagacg
     4981 cttcggcctg ttgccgtttt gccatttcag cagcgtggat gcgagcaccg agtacagaaa
     5041 gtgctttagc tttgttttta tgttgtgaac gttcgtcctg acattcaaca acaatcccgg
     5101 tcggcaagtg agtaatacga attgccgaat cggtggtgtt aacgtgctga ccacccgccc
     5161 ctgacgagcg gaaagtatca atgcgtaaat ctgctgggtt gatgtccggc agttctgcat
     5221 caggcagttc tggcattacc gcaacggtac aagcagaagt atgaatacga ccctgcgatt
     5281 ccgtagcagg aacacgctgc acgcgatgac cgccagattc gaatttcaaa cgaccatata
     5341 caccatcacc gctaattttg gcgatgatct ctttataacc accatgttca ccctcgctgg
     5401 cgctcatgat ttctacacgc cagcggcggg cttcggcgta acggctgtac atacggaaca
     5461 gatcgccagc gaacagcgcc gcttcgtcgc cgccggttcc ggcacggact tcgaggaagg
     5521 cgttacgttc gtcatcagga tcttttggta gtaacagaac ctgtaattgc tgttccagtt
     5581 gctcgctttt ttctttagct tcgcgcagtt catcctgcgc catctcacgc atttcaggat
     5641 cgtcgagcat catctgtgcg gtttcgatat cttcctgaac ctgttgccag tcggtaaaac
     5701 agcgcgaaac atcacttaac tgcgcatatt cacgagataa tgcgcgaaaa cgttcctggt
     5761 cagcgatggt ttgcgcatca cccagcagcg cctgaacttc ttcatggcgt tcatgcaggg
     5821 cttccagttt ggcaacgata gaaggcttca taggcgtaaa tgcaccctgt aaaaaaagaa
     5881 aatgatgtac tgctactcca gcccgaggct gtcgcgcaga atattcaggc gttcgttatc
     5941 cccgtcacgg gcggcctgtt gaagtgattt tgttggtgca tggatcaggc ggttagtcag
     6001 tttccatgcc agatcctgca taatggtttg cgcatcgccg ccctgttcaa gggccgctaa
     6061 cgctttagca gttaactcat cacgaacctg ctctgcctgg ctacgataat cgcgaatagt
     6121 ttcgctggcg ctttgtgctc gaagccatgc cataaattca ctggcctcct gggcgacgat
     6181 agtttccgcc tccacagcag cagctttccg ttgcgccagg ttgtgcgaaa taatgctttg
     6241 cagatcgtca acgctataaa gataagcatt cgccagttta ccaacttccg gctcaacatc
     6301 gcgcggaacg gcaatatcca ccagcagcat tggttgattg cgacggcttt ttaatgcgcg
     6361 ctctaccatg cctttaccga taatcggcaa cgggctggcg gtggaactga tgatgatatc
     6421 ggcttcgcgc agacgttcgt cgatatcgct cagggcaatc acttccgcgc caacctcatc
     6481 tgccagaatt tgggcacgtt cgcgagtgcg attggcgata atcatcttct gtactttgtg
     6541 ttcgcgcaga tgacgcgcca ccagctcgat ggtttcgccc gcgccaacca gcagtaccgt
     6601 caccgtagag agcgattcaa agatctgccg cgccagcgta caagccgcaa aagcgacaga
     6661 cacagcactg gcaccgatat ctgtttcagt gcgaacgcgt ttcgccacag agaaagattt
     6721 ctgaaacatg cgttccagtt cgctggcctt catatgacct ttttgcgaat cggcaaacgc
     6781 ttttttaacc tgaccgagga tctgcggctc gccaaggacg agagaatcaa gcccactggc
     6841 aacgcgcatt aaatgactga ccgcatcgtt atcctgatgc cagtagaggc tattacgcag
     6901 atcgtcttcg ttgagattat ggtaatcaca caaccagcga atgagcgcct cttgcaggtc
     6961 atcccgttct tcaacgctga gataaagttc tgtgcggtta cacgtcgaca gcaccacacc
     7021 gccctgcacc atcggctgcg cgagcagact gtcaagcgcc tgatcgagct tatccggcga
     7081 aaacgataca cgttctcgca gcgatacagg tgccgtttta tggttgatac cgagtgctaa
     7141 aagggtcatg tctgcgggaa ataataccaa cgttgatagg gttagtctgc ttgcatcata
     7201 caggatgcgt aagatcaata aaagagagcg cccccttttg gagtaattgc cgaaagccgt
     7261 tagattctgg caattaagac aacgtgaaca tagacgatgg ctaacggtaa cgctagcatt
     7321 aagggttata actgcaacgt atctcaagga cttgtcatta ttatgcccct gcccgatttt
     7381 cgtcttatcc gcctgctacc gctggctgct cttgtgctca ctgcctgttc cgttaccacg
     7441 cccaaaggtc ctggcaaaag cccggattcg ccacaatggc gtcagcatca gcaagacgtg
     7501 cgcaatctta atcagtatca aactcgcggc gcgttcgctt atatttctga ccaacaaaaa
     7561 gtgtacgccc gtttcttctg gcaacaaact ggccaggatc gctaccgtct gctgctgact
     7621 aacccattgg gcagcacgga actggagctg aatgctcaac cgggtaacgt gcagttagtc
     7681 gacaataaag gtcagcgtta tacctccgat gacgccgaag agatgattgg caaattgacc
     7741 ggaatgccaa ttccgctcaa cagcttgcgc cagtggattt taggtttacc gggtgatgcg
     7801 actgactaca aacttgacga ccagtaccgc ctgagcgaaa tcacctacag ccagaatggc
     7861 aaaaactgga aagttgttta cggtggttat gacacaaaaa ctcaacctgc gatgccagcc
     7921 aatatggaac tcaccgacgg tggtcaacgc atcaagttaa aaatggataa ctggatagtg
     7981 aaataatgcg gacacagtgg ccctctccgg caaaacttaa tctgttttta tacattaccg
     8041 gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt tcagtttctt gattacggcg
     8101 acatcatcag cattgagctt cgtgacgatg gggatattcg tctgttaacg cccgttgaag
     8161 gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg gttgttgatg aaaactgcgg
     8221 cagacagcgg gcgtctttcg atgggaagcg gtgcgaatat cagcattgac aagcgtttgc
     8281 cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc gacggttctg gtggcgttaa
     8341 atcatctctg gcaatgcgga ctaagcatgg atgaactggc ggaaatgggg ctgacgctgg
     8401 gcgcagatgt tcctgtcttt gttcgtggtc atgccgcgtt tgccgaaggc gttggtgaaa
     8461 tactaatgcc ggtggatccg ccagagaagt ggtatctggt ggcgcaccct ggtgtaagta
     8521 ttccaacacc ggtgattttt aaagatcctg aactcccgcg caatacgcca aaaaggtcaa
     8581 tagaaacgtt gctaaaatgt gaattcagca atgattgcga ggttatcgca agaaaacgtt
     8641 ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata cgccccgtcg cgcctgactg
     8701 ggacaggggc ctgtgtcttt gctgaatttg atacagagtc tgaagcccgc caggtgctag
     8761 agcaagcccc ggaatggctc aatggctttg tggcgaaagg cgttaatctt tccccattgc
     8821 acagagccat gctttaagcc gggcaagctg agtttcggtg acaacgtcac cttgttccag
     8881 acgttgcatc gcgctcttta atacaccgcc tggaaagaat catgcctggc ccgcacagtt
     8941 ttcggcagat tctttccacc aatggacgca tgcctgaggt tcttctcgtg cctgatatga
     9001 agctttttgc tggtaacgcc accccggaac tagcacaacg tattgccaac cgcctgtaca
     9061 cttcactcgg cgacgccgct gtaggtcgct ttagcgacgg cgaagtcagt gtacaaatta
     9121 atgaaaatgt acgcggtggt gatattttca tcatccagtc cacttgtgcc cctactaacg
     9181 acaacctgat ggaattagtc gttatggttg atgccctgcg tcgtgcttcc gcaggtcgta
     9241 tcaccgctgt tatcccctac tttggctatg cgcgccagga ccgtcgcgtc cgttccgctc
     9301 gtgtaccaat cactgcgaaa gtggttgcag acttcctctc cagcgtcggt gttgaccgtg
     9361 tgctgacagt ggatctgcac gctgaacaga ttcagggttt cttcgacgtt ccggttgata
     9421 acgtatttgg tagcccgatc ctgctggaag acatgctgca gctgaatctg gataacccaa
     9481 ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg cgctatcgct aagctgctga
     9541 acgataccga tatggcaatc atcgacaaac gtcgtccgcg tgcgaacgtt tcccaggtga
     9601 tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact ggtcgatgat atgatcgaca
     9661 ctggcggtac gctgtgtaaa gctgctgaag ctctgaaaga acgtggtgct aaacgtgtat
     9721 ttgcgtacgc gactcacccg atcttctctg gcaacgcggc gaacaacctg cgtaactctg
     9781 taattgatga agtcgttgtc tgcgatacca ttccgctgag cgatgaaatc aaatcactgc
     9841 cgaacgtgcg tactctgacc ctgtcaggta tgctggccga agcgattcgt cgtatcagca
     9901 acgaagaatc gatctctgcc atgttcgaac actaatcgaa cccggctcaa agacccgctg
     9961 cggcgggttt ttttgtctgt aatatccatt tgtatgacct atgcctcctt cacctgccat
    10021 ttagttgaca gatgatgcgc tcacggatga aacattattg tgaacaaaat attttcctca
    10081 catgtgatgc ctttccgcgc tctgatcgac gcttgctgga aagaaaaata tactgccgca
    10141 cggtttaccc gtgacctgat tgccgggata accgtcggga ttattgctat cccgctggcg
    10201 atggcgttgg ctattggtag tggtgtggcc ccccagtacg gtttatatac cgcagctgtt
    10261 gcggggattg tcattgctct gacggggggg tcacgcttta gcgtttccgg tccgactgcg
    10321 gcatttgtgg taattctcta tcccgtttcg caacagtttg gactggcagg actgctggtt
    10381 gcgaccttgc tgtcgggaat ctttttgatt ctgatggggc tggcacgctt tggtcgcctg
    10441 attgagtata ttccggtttc cgtcacctta ggtttcacct cgggtatcgg gatcaccatc
    10501 ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg cccatgtccc ggaacattat
    10561 ctacaaaaag tcggcgcatt atttatggcg ctgccgacca ttaatgtggg tgatgcagcc
    10621 attggcattg tgacgcttgg cattctggtt ttctggccgc gtctgggcat acgtttaccc
    10681 ggccaccttc cggccttgct ggctggttgc gcggtgatgg ggattgttaa cctgctcggc
    10741 ggacatgttg ctaccatcgg ttcgcaattc cactacgtcc tggccgatgg ttctcagggt
    10801 aacggtattc cgcaactgct gccgcaactg gtgctgccgt gggatctgcc taattcagaa
    10861 ttcacgctaa cctgggattc tattcgcacc ctgctgcctg cggcattctc aatggcaatg
    10921 ctcggcgcaa tcgaatctct gctctgcgcc gtggtgctgg atggtatgac cgggacgaaa
    10981 cacaaggcga acagcgaact ggttggacag ggactgggga atattatcgc tccgttcttt
    11041 ggtggtatta ccgctacagc tgccatcgcg cgttctgccg ctaacgtccg tgccggggca
    11101 acttccccta tctcggcggt gatccactct attctggtta ttcttgccct gctggtactg
    11161 gcaccgctgc tctcctggct gccgctttcc gccatggcag ccctgctgtt gatggtggcg
    11221 tggaacatga gtgaagcgca caaagtggtc gacttgctgc gtcatgcacc gaaagatgac
    11281 atcatcgtca tgctgctgtg catgtcgctg accgtgctgt tcgatatggt tattgccatc
    11341 agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc gtatcgcacg tatgactcgc
    11401 ctggcaccgg tagtcgtaga cgttccagac gatgtcctgg ttctgcgcgt tattggcccg
    11461 ctgttttttg ctgccgctga aggcttattc acggacctgg agtcacgtct tgaaggcaaa
    11521 cggattgtga ttctgaagtg ggatgccgtt ccggtacttg atgctggtgg tcttgatgcg
    11581 ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac tgcgcgtgtg caacctggaa
    11641 ttccagccac tgcgcactat ggctcgcgca ggcattcaac cgatcccggg acgcctcgca
    11701 ttcttcccga atcgtcgcgc ggcgatggcg gatttataag tacgagattg accagtcagc
    11761 gcaaactgac tggtcagcaa actgcattat ttgttagcta tgacggcgat tatcgctacg
    11821 gcggcaacgt ttgtcatagt ggcgaaccca ccagtaacgg tcgcacacct gttcatgccc
    11881 gccaacacgg gcacctgcca gccagagaat cgcaccgacg aaaatactta gcacagcacc
    11941 atgtgcgaaa tactggggca tattaaactg tggtaactgg ttgaggattg aataccccac
    12001 gccgaccacc attaccacca gacccaaccc catgagcacg ttaccgagta acgaagcgtt
    12061 tttgcgtttc atatgtcacc tccggaactt tctgggttgt aacagggaat acccctcttc
    12121 cttatgcgta aagtatagac aacaccctgt gggttatgtg cgggtgtgat cacaattaca
    12181 acccttactt caacaaaact ttacaaataa acgcctgaac accttgactt tctaatagtc
    12241 cactcaggca ataattgcat tatctacatt tgatacgcag ttttttgtca agcggggccg
    12301 caaccagtaa actacgcgcc agttatgtac acactcagga caaaaaaacg tgacgattaa
    12361 attgattgtc ggcctggcga accccggtgc tgaatacgcc gcaacgcgac ataatgctgg
    12421 tgcctggttc gttgacttac tggcagagcg attgcgcgct ccgctgcgcg aagaggctaa
    12481 attctttggt tatacttcgc gagtcaccct tggaggcgaa gatgtccgcc tgttagtccc
    12541 gactacattt atgaatctca gcggcaaagc cgttgcggcg atggccagtt ttttccgcat
    12601 taatccggac gaaattctgg tggcccacga cgaactggat ctgcctcctg gcgtcgccaa
    12661 atttaagttg ggtggcggtc atggtggtca caatggactg aaagacatca tcagtaaatt
    12721 gggtaataac cctaactttc accgtttacg catcggaatc ggtcatccgg gcgataaaga
    12781 taaagttgtc ggttttgtgt taggcaaacc gcctgttagt gaacagaagt taattgatga
    12841 agccatcgac gaagcggcgc gttgtactga aatgtggttt acagatggct tgaccaaagc
    12901 aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt gtctgcggca tttttgccga
    12961 gtgccgtgta taataggcaa agttatttcc atttctgcaa tctgttagca ataacaggtt
    13021 gattattaag atattaaggt gatttaaatc atgggattca aatgcggtat cgtcggtttg
    13081 cccaacgtcg ggaaatctac cctgttcaac gcgctgacca aagccggtat tgaagcggcc
    13141 aactttccat tctgcaccat cgagccgaac actggcgtcg taccaatgcc tgaccctcgc
    13201 ctggatcaac tggctgaaat cgtaaaaccg cagcgtacac ttcccacgac catggaattt
    13261 gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg aaggtctggg taaccagttc
    13321 ctgaccaaca tccgtgaaac cgaagcgatc ggtcacgttg ttcgctgctt tgaaaatgac
    13381 aacatcattc acgtttccgg caaagttaac ccggcggatg atattgaagt tatcaacacc
    13441 gaactggcgc tggcggatct cgacacctgt gaacgtgcga ttcatcgcgt acagaagaaa
    13501 gccaaaggtg gcgataatga cgcgaaagcc gagctggcgg tactggaaaa atgcctgccc
    13561 cagctggaaa acgcaggtat gctgcgtgcg ctggatttaa gcgctgaaga gaaagcggct
    13621 gttcgttacc tgagcttcct gacgctaaaa ccaacaatgt acatcgccaa cgtcaacgaa
    13681 gacggttttg aaaacaaccc gtatcttgac caggtgcgtg aaatcgcagc gaaagaaggt
    13741 tctgttgtag ttccggtttg tgctgctgtt gaagcagaca ttgccgaact ggacgacgaa
    13801 gagcgtgacg agtttatgca ggagcttggg ctggaagagc cgggcctgaa ccgtgtgatc
    13861 cgtgccggtt ataagctgct gaacctgcaa acttacttca ccgctggggt gaaagaagtg
    13921 cgtgcatgga ccattccggt tggtgcaacc gcgccgcagg cagcgggcaa aatccatact
    13981 gattttgaaa aaggctttat ccgtgcacaa accatctcgt ttgaagattt catcacttac
    14041 aaaggtgaac aaggcgcgaa ggaagcaggc aaaatgcgtg cagaaggtaa agactacatc
    14101 gttaaagatg gcgatgtgat gaacttcctg ttcaacgtct aattccgcgt aatatcctgg
    14161 cggttaataa cgagccatta tgtgagttgc acgtaatggc ttttttacgc ctgctacgcg
    14221 tcttctttca attctgagtt accaaaactc ataatcagac tcctgacaat attaacaatg
    14281 cagcttttat gtgataacaa tcacagtgca ttaaccgttc aagagtttgt catgaaaaaa
    14341 acatttacct tcagcctgtt agcaattgtc tttgcaagcc tgttgagtgc ttgtgttccc
    14401 caccatcatc accgccacag cgatggccct cgtggtcctg cgccctcagg taagccaatg
    14461 ccaccttcaa atggtggacc aaatcacggc agtcatggtc gctattaatt agcaattcat
    14521 agcgggaaag tacatactct cccgctatcc acggcgctta aactgccctg catcaatgcc
    14581 atgttgcttc attttccgcc acagcgtagt gcggccgatc ccaagtaaag ccgacatttc
    14641 ctgaatgcga ccgcctgtga cctgggctgc gttaataatt gcctcttttt caacttccgc
    14701 aaatgacaga ctggtggaaa ggcgggtggc gctgacatca tctgtcgcct gctcggtaaa
    14761 cagatgttcc ggcaaatcac tgacgcgaat gcgcccatta tcactactca gagcaagatt
    14821 ctcgatgacg ctgtaaagtt caaaatcgtt gccaggccat gcacaggaaa ccaggcgagc
    14881 gagggcatcg tcatcaattt tcagccgcgt agagaagcgt ttttcaaggc tgcgtaattt
    14941 gttattcacc agcgccggaa tgctgccacg ccgcatacgc agtggcggga tggtaatttc
    15001 aaatgcatgc agcgcgtaat acagctggcg actaaaacga ttttgttcca ccagcattgc
    15061 gaggtccgcg gtcgttgtgg caatcacttt gacatcaatg ggtattaaac gccgcgcatc
    15121 cagtcgcgtg ataaccccct gcttgataac ctgaagcaag gcagactgta actccaccgc
    15181 cagatattca atcttttcaa gaaacagcgt gccgccgtgc gccagttcca gccgactcag
    15241 acgaccattt tcactatccg tgcgatcgcc accaataaat tcttccgcca gcgccgcatc
    15301 accatataac tcacaattga cggcgatata aggccccgct gcacgctcac tttcattatg
    15361 aattgcctga cttagcagtg ccttgcccac gccctcttct ccacaaagca ggacaggaaa
    15421 gctactgcgt gccgcctggc gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg
    15481 tggcatatga gcgaaggtat ggctgacttt tcctaattga ctggtcatta actgccgcat
    15541 ctgttccaca ggatggagca ataaaataaa gctggttccc tgcgtttcta ttatcggttt
    15601 aagggttatc accgcatcaa taaactgatg ctgactttca aaggtggctt ctacgtgttt
    15661 gagcggatgt gcctgtttta ttgcttgttg caatacggcg ggtaacgtta agagttcagt
    15721 gattgccctt ccctgacttg ccgtcgcgtc aaggcgcaag actcgcgccg cctgggcgtt
    15781 gataaattgc aaattaccct gctcgtccca gctaatcacg ccatcatcca tactttctaa
    15841 cagggcatta agttgattta aatgacggtt agtttcagcg agcaaactgt ccgtcagcag
    15901 taaatttccg acctcgcgtg cgattgccaa cgtcaacggc aaatcagctg cggtagtttg
    15961 ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg
    16021 cgttgcgcaa aaggcccagt tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt
    16081 cacggcctga ccagagatag ccgctaacga tagcgcacaa gtaccaataa ttccctcggc
    16141 gcaatacgtg ccgtcattga atcccagtac acttagctgc tgcaaggttt gcggatcgcc
    16201 attacggctg agaatgcagg cggtttcatc gaggataagc aacgcacact ctcgcggtgc
    16261 catatattcc caggcatctt ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg
    16321 ccgataaata gaagcaaatg tcacgccctg ggcctggtgt ggtacgttcc atgtctcccg
    16381 tttcatcagc ttattgcatc tctcccagga ggttgcaatt aagggagata tgccccgacc
    16441 atcgttgtta aaagcgccac tcatatcccg catcccttat gacaaaaggt gttccggctc
    16501 gcaattttaa gtattaatac acgtaaaagt ttcaatatgg aacattcatt aaagcatatg
    16561 tttcactatg aaacataatt tgagctaatt ttcttttccc ttgcctgatg cacaatggat
    16621 tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc catgccctac cgtaattgct
    16681 ggagcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga cgtactggac gaacaactgg
    16741 caggactagc gaaagcgcat ccatcgctga cactgcatca ggatccggtg tatgtcaccc
    16801 gagctgatgc ccccgttgca ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg
    16861 agccgatgca ttgtggttat atcggtcagg ggatgctttc tggggcctgt ccgggcgaaa
    16921 ttttcacctc accgacgccc gataaaatct ttgaatgcgc catgcaaatt gatggcggcg
    16981 aaggtgtact gttgattatc aaaaattaca ccggcgatat tcttaacttc gaaacagcaa
    17041 ccgagttact gcacgatagc ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg
    17101 cagtaaaaga cagtctttat accgcagggc ggcgcggcgt tgccaacacc gtattaattg
    17161 aaaaactcgt aggtgcagcg gcggagcgtg gcgactcact ggacgcctgt gcggaactgg
    17221 ggcgtaagtt gaataatcaa ggccattcaa taggtatcgc tctcggtgcc tgtaccgttc
    17281 ctgccgcggg caaaccttct tttaccctgg cggataatga gatggagttt ggcgtcggca
    17341 ttcatggtga gccaggtatt gaccgccgcc ccttctcttc ccttgatcaa accgtcgatg
    17401 aaatgttcga caccctgctg gaaaatggct cataccatcg cactttgcgt ttctgggatt
    17461 atcaacaagg cagctggcag gaagaaccac aaaccaaaca accgctccag tctggcgatc
    17521 gggtgattgc gctggttaac aatcttggcg caactccgct ttctgagctg tacggcgtct
    17581 ataaccgcct gaccacacgt tgccagcaag cgggattgac tatcgaacgt aatttaattg
    17641 gcgcgtactg cacctcactg gatatgaccg gtttctcaat caccttactg aaagttgatg
    17701 acgaaacgct ggcactctgg gacgcccctg tccacacccc ggcccttaac tggggtaaat
    17761 aaggagaaag caatgtcact gagcagaact caaattgtta actggctcac tcgttgtggc
    17821 gatattttca gcaccgagag cgagtatctt actggactgg atcgcgagat tggcgacgct
    17881 gaccacgggc taaatatgaa tcgaggcttt agcaaagtgg tggaaaaact ccctgctatc
    17941 gcagataaag atatcggttt cattctcaag aataccggca tgacgctact ttccagcgtc
    18001 ggaggtgcca gtggtccgct gttcggtacc ttctttatcc gcgccgcaca gacaacccag
    18061 gcacggcaaa gcctgacgct ggaagagctt tatcagatgt tccgcgatgg cgcggacggc
    18121 gtaatcagtc gcgggaaagc cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg
    18181 gtggtggaat cgttacgtca gtcctgcgag caaaatctcc ctgttccggc ggcgctcgac
    18241 gctgccagta gcatcgccga atccgctgca caaagtacga ttacgatgca agcccgcaaa
    18301 ggccgcgcca gttatctcgg tgaacgcagt attggtcacc aggatcccgg cgcgacctcg
    18361 gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag agtaaggaat tggtgatggt
    18421 aaacctggtc atagtttcac atagcagccg actgggagaa ggtgtcggtg aattagcccg
    18481 tcagatgtta atgagtgata attgtaaaat cgccattgcc gcgggaattg acgatccaca
    18541 aaatcccatt ggtaccgatg ccatcaaagt gatggaggcc atcgaatctg ttgccgatgc
    18601 cgaccatgtg ctggtcatga tggatatggg tagcgcatta ttgagtgctg aaactgcgct
    18661 ggaattgctg gctcccgaga tcgccgcaaa agtacgtttg tgtgctgcgc cgttggtcga
    18721 aggtacactg gcagcaacgg tcagcgcggc ctcgggggcg gatatcgaca aagtgatctt
    18781 tgacgccatg catgcgctgg aagccaaacg tgaacaactg ggtttaccgt cctccgacac
    18841 tgaaatctct gacacatgtc ctccgtacga tgaagaagcc cgttctctgt cggtggtcat
    18901 aaaaaaccgt aacggcctgc atgtacgtcc ggcctcccgg ctggtttata ccttatcgac
    18961 atttaatgcc gatatgttgc tggagaaaaa cggcaaatgc gttacaccgg agagtattaa
    19021 ccagattgcg ttactacaag ttcgctataa cgatacgctg cgcctgattg cgaaaggacc
    19081 agaagctgaa gaggcactga tcgctttccg tcagctggct gaagataact ttggtgaaac
    19141 ggaggaagtt gctccaccta ctctgcgtcc cgttccgcct gtttcgggta aagcctttta
    19201 ttatcaacct gttttatgta cggtacaggc aaaatcaacc ctgaccgtgg aagaagaaca
    19261 agaacgatta cgccaggcca ttgacttcac gttattagat ctgatgacgt taacagcgaa
    19321 agcagaaacc agcgggcttg acgatattgc cgcaatcttt tctggtcacc atacactgtt
    19381 agatgatccg gaactgcagg cggcggcaag cgaactcctt cagcatgaac attgcacggc
    19441 ggaatatgcc tggcagcaag ttcttaaaga acttagccag caataccaac agctggatga
    19501 tgaatatcta caggctcgat atatcgatgt ggacgatctt ctgcatcgca ccctggtcca
    19561 cctgacccaa acgaaagaag aactcccgca gtttaactcg ccaactattc tactggcgga
    19621 gaacatttat ccctccacag tactgcaact ggatccagcg gttgtaaaag gtatctgcct
    19681 tagcgccgga agtccgctat cccacagtgc cctaatcgcc cgtgaactgg gaattggctg
    19741 gatttgccag cagggtgaga aactgtatgc gatacaacca gaagaaacgc taacgctgga
    19801 cgttaaaacg caacgtttca accgtcaggg ttaaaccacc gttcggatgg catcattctg
    19861 atgtcatccg gcaatttact ctgcactgat gttttta
//
LOCUS       DAZFRG010000073        19852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFRG010000073 DAZFRG010000000
VERSION     DAZFRG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.073"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(138..842)
                     /locus_tag="ACHKUC_002055"
     CDS             complement(138..842)
                     /locus_tag="ACHKUC_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6618310.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(979..1431)
                     /gene="moaE"
                     /locus_tag="ACHKUC_002056"
     CDS             complement(979..1431)
                     /gene="moaE"
                     /locus_tag="ACHKUC_002056"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO6618311.1"
                     /translation="MAETKIVVGPQAFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1433..1678)
                     /gene="moaD"
                     /locus_tag="ACHKUC_002057"
     CDS             complement(1433..1678)
                     /gene="moaD"
                     /locus_tag="ACHKUC_002057"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO6618312.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1671..2156)
                     /gene="moaC"
                     /locus_tag="ACHKUC_002058"
     CDS             complement(1671..2156)
                     /gene="moaC"
                     /locus_tag="ACHKUC_002058"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO6618313.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2159..2671)
                     /gene="moaB"
                     /locus_tag="ACHKUC_002059"
     CDS             complement(2159..2671)
                     /gene="moaB"
                     /locus_tag="ACHKUC_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO6618314.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2693..3682)
                     /gene="moaA"
                     /locus_tag="ACHKUC_002060"
     CDS             complement(2693..3682)
                     /gene="moaA"
                     /locus_tag="ACHKUC_002060"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO6618315.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3670..3814)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4079..4987
                     /gene="yvcK"
                     /locus_tag="ACHKUC_002061"
     CDS             4079..4987
                     /gene="yvcK"
                     /locus_tag="ACHKUC_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO6618316.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKEDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5025..7046)
                     /gene="uvrB"
                     /locus_tag="ACHKUC_002062"
     CDS             complement(5025..7046)
                     /gene="uvrB"
                     /locus_tag="ACHKUC_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO6618317.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGNFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7624..8301)
                     /gene="bioD"
                     /locus_tag="ACHKUC_002063"
     CDS             complement(7624..8301)
                     /gene="bioD"
                     /locus_tag="ACHKUC_002063"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6618318.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8294..9049)
                     /gene="bioC"
                     /locus_tag="ACHKUC_002064"
     CDS             complement(8294..9049)
                     /gene="bioC"
                     /locus_tag="ACHKUC_002064"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO6618319.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHIHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9036..10190)
                     /gene="bioF"
                     /locus_tag="ACHKUC_002065"
     CDS             complement(9036..10190)
                     /gene="bioF"
                     /locus_tag="ACHKUC_002065"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO6618320.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCYDTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10187..11227)
                     /gene="bioB"
                     /locus_tag="ACHKUC_002066"
     CDS             complement(10187..11227)
                     /gene="bioB"
                     /locus_tag="ACHKUC_002066"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO6618321.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11314..12603
                     /gene="bioA"
                     /locus_tag="ACHKUC_002067"
     CDS             11314..12603
                     /gene="bioA"
                     /locus_tag="ACHKUC_002067"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO6618322.1"
                     /translation="MTTDDLTFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            complement(12714..13844)
                     /locus_tag="ACHKUC_002068"
     CDS             complement(12714..13844)
                     /locus_tag="ACHKUC_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6618323.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            13953..14429
                     /locus_tag="ACHKUC_002069"
     CDS             13953..14429
                     /locus_tag="ACHKUC_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO6618324.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            14581..15864
                     /locus_tag="ACHKUC_002070"
     CDS             14581..15864
                     /locus_tag="ACHKUC_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO6618325.1"
                     /translation="MNTFSVSRLALTLAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(16005..18266)
                     /locus_tag="ACHKUC_002071"
     CDS             complement(16005..18266)
                     /locus_tag="ACHKUC_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO6618326.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELLKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPNFDVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(18449..>19852)
                     /locus_tag="ACHKUC_002072"
     CDS             complement(18449..>19852)
                     /locus_tag="ACHKUC_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO6618327.1"
                     /translation="LILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIVGLVIKPFPEP
                     VVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAAFVTTGLGKRI
                     AYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSVAVALGSEPEK
                     SPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWGGWALAAGLP
                     GIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVFVLALLGWI
                     FSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGLSSLLSKVK
                     FFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPVFAMLANVS
                     GAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVH
                     ITLGVWWWNMLIGWNML"
BASE COUNT         4708 a         5357 c         5030 g         4757 t
ORIGIN      
        1 atacttttgc atttatggat acagaaaacg cttccgctcg tccataaggc atattttata
       61 cctggtactt gttaagtgtc gtttttaata agaaaaaaat cccgacacat ggccgggata
      121 ttgatgatga aaaagaatta acggcggttg ccgaagatcc gcagcaacat caggaacagg
      181 ttgatgaagt ccagatacaa ggttaacgcg ccaagaatgg aatatttgcg caggttcgac
      241 gtgtcgcgag tatcaatctg ctcacccata tttttcagtt tctgggtgtc atacgccgtc
      301 aatccgacaa agacaatcac gccgatgtag gtgactgccc acatcaatgc ttcgcttttc
      361 agccagaagt taaccagcga tgccagcaca atgccgatta acgccataaa cagcatattg
      421 ccaaagccgc ttagatcgcg cttcgtggtg taaccgtaca ggctcattgc gccgaacatc
      481 ccggcagtaa cgacgaaagt actggcgata gaagcagcgg tatagacaat gaaaatactg
      541 gaaagcgtaa gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg
      601 ctcagctttt gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga
      661 ccgattaaaa agacacggtt agtaaacaac agctccatca cggccgcgga attagccgca
      721 taccaggcaa caaatgctgt cagcaacaag ccaacggtca tccagccata gacttgcgcc
      781 atataagttt gcaagccagc ccggggttgt acgattgaat cagaacgtgg gaatctgtcc
      841 atgactttct cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt
      901 gcatcgacgc agcgtaaacg tatgtactga gcggtgaaat tgccggacgc agcggtgcct
      961 tatccggcta acaataaact accagcgttt tgccgcctgc tgatcgctct cccgagcttc
     1021 gacccagcgg tcgccttccg gcgtggcttc gcgcttccag aacggtgcgc gggttttgag
     1081 ataatccata ataaactgcc cggcttcaaa cgcactgctg cgatgcgcac tggtaacacc
     1141 gacaaaaaca atttcatcgc ccggccataa ttcaccaatg cggtgaatta ccgtgacgcg
     1201 ccccagcggc cagcggttac gcgcttcatc aacgatttct gccagtgctt tttctgtcat
     1261 tcccggatag tgttcgaggg ttaatgcttt gacgctgtcg cccaggttat ggttgcgcac
     1321 cttaccggta aaggtgacta ccgcaccgtc ttcgtcacgc tcagccagcc acgggtactc
     1381 ttctcctacg ctgaacgcct gcgggccgac aacaattttg gtttctgcca tcttaacctc
     1441 cggttaccgg cgggaagaaa gccacttcgt cgccgtcagt cagcgaatgg tcaaaactca
     1501 ccagcgtctg gttgacagca gccagcaatt tgccatcttc cagcgccagc gcccagcgat
     1561 cgctctgcgc agccagatgt tggcgtaacg cttcaacggt tgggaaatcc gcagccactt
     1621 cggttgcatc tgttcccacc aactcgcgca cctgggcgaa aaaaagaacg ttaatcatcc
     1681 gcttccacct taaagtcacc cgacttgccg ccgctcttcg ccagcaaacg taccggacca
     1741 atcaccatat ctttttgcac cgctttgcac atgtcataaa tggtcagcgc cgccacggag
     1801 gccgcggtta acgcttccat ttcgacaccg gttttcccgg tcaggcggca taaggtttct
     1861 atacgtaccc gattgtgctc cggctcggcc tgtaaattga cttcaacttt gctgagcatc
     1921 agcgggtgac acagcgggat cagatcccag gtgcgttttg ccgcctgaat accggcgata
     1981 cgcgcagtgg caaatacgtc gcctttgtgg tggcgaccat caataatcat cgccagcgtc
     2041 tcgctgcgca tggtgacaaa ggcttcggcg cgcgcttcac gcacggtttc cgctttggcg
     2101 gagacatcca ccatgtgcgc ttcgccagcg gcgttgatat gggtcagttg cgacatactt
     2161 atttcttcaa atgtggatgg aaattacacg gacgcgtacg ggcatccagc tgtggcgcaa
     2221 tgatattttc ccatgcggta cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg
     2281 ttttgttagc gacgcccgct accgcacgag attgcaacgt ggaagtgcca atctcttcga
     2341 acgacaacat ccggaacact tcaccaaaac cttcaacttc acggtcgaac aacggcagca
     2401 gcgcttcggg agcctgatcg ccttccgtca ggccagtacc acccgtaatc aacaccactt
     2461 gtacatcatc gctggcgatc cacgcagata cctgagcgcg aatagcgtag cggttttctt
     2521 tcacaatggc tttatcgaca acgtgatggc ccgcttcttg cgccgaatcg cgcagatagt
     2581 gaccggaggt atcgtcttct tcaccgcgac gattagaaac cgtaagaata gcaatacggg
     2641 tcgggataaa ttcagtgctt acctgactca tctgatctct ccttttgacg ttttagccac
     2701 caatgtacga taagttttgc gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt
     2761 ttttctcccg cagcgccgct gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt
     2821 ccagcagatc gcgcaggtta acgccgcctt caccaaacag gcagagatgg agtttaccaa
     2881 tggaggaaac gcgcaggcgg ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa
     2941 ggccaatctc tccggcgtaa tccggatgac aaaagacttg cgcgggaccg tcgctgcgtt
     3001 gacgtaattg gtggatccag ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga
     3061 tgtgatgctt acggaagagc tcgctgccct cgcccgtttc catcagttcg atgaaacgca
     3121 gctggatggg acgatgctgg atccagttca gaaaggtgtc gagctggtga tgattaacat
     3181 cacgcatcag cacggtattg actttgacct tctcaaaacc ggcctcaaat gcagcatcga
     3241 ttcctgccat gacctggttg aatttatcct gcccggtaat ggcatgaaac tggcgagcgt
     3301 ccagactgtc gacgctgacg ttgatgccag taagtccagc atcgcgccag ttcgccacat
     3361 cgcgctccag acggtaacca ttggtggtga ccgctatctg gcgaatagcg tcgttttctc
     3421 gcacagcggc gatgatatcg gtaaagtcgc ggcgtaaaga tggctcacca cctgtcagac
     3481 ggactttttc ggtgcccaaa ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg
     3541 tcagaaagcc tttattggtg acgccgctcg gcttgtaacc atccggcagg cagtaggtgc
     3601 aacgaaagtt acacacatca gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg
     3661 catcagtcag ttgtgaagcc atgtacacct ttccagatac gggaggcgaa gtcatttctt
     3721 ccttcgccct ggtggcaatc tttacgagca ttgtcacggc caaagcaccg tatcagttga
     3781 cccaggtgca gaggctagag tgtttagtgg ttaagccgat actagcgtgt aaatgagatt
     3841 tttaccatcc acactttcgc tatgtaatca tgtatatagc gtcatgatcg tgctatttcg
     3901 ctacagaata agggaaaatg gcggttttac tttgatatac atcatcaggg aggcatagcg
     3961 tgcatcgtcc tttagaggta gaagaaaata gtttgtatcc tcaatattgg caggttaatt
     4021 gctgtttcct cgcaatttgc gctactgtag cgcggataat ctgactccag gaaactatat
     4081 gcgcaatcgt acgctggctg atcttgatcg tgtcgttgct ctcggcggag ggcatggact
     4141 gggacgcgtt ctctcatcac tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac
     4201 caccgataac ggtggctcga cggggcgtat tcgccgctca gaaggcggca ttgcctgggg
     4261 cgatatgcgc aactgcctca atcagctgat aactgaaccc agcgtcgctt ccgcaatgtt
     4321 tgaatatcgt ttcggcggta atggcgaact ttccgggcac aaccttggaa acctgatgtt
     4381 aaaggcactg gatcacctta gcgtgcgacc tcttgaagcg atcaatctga ttcgaaatct
     4441 gctgaaagtg gacgcacatt tgattccgat gtcggagcat cctgttgatc tgatggcgat
     4501 tgacgatcag gggcatgaag tatacggcga ggtcaatatc gaccagttaa ctgcgccgat
     4561 tcaagagtta ttgttaacgc cgaatgtacc cgcaacgcgt gaggcggttc acgctatcag
     4621 tgaagcggat ctcatcatta ttgggcctgg cagtttttac accagcctga tgccaattct
     4681 gctgctgaag gaaatcgcac aggcattacg ccgcacgcca gcgccgatgg tctatatcgg
     4741 caatctgggg cgtgagttga gtttacctgc ggctaatctg aagctggaaa gcaagctggc
     4801 aattatggag cagtatgttg gtaaaaaggt aattgatgcg gtcatcgtcg ggccaaaaga
     4861 ggatgtctcg gcggtgaaag agcggattgt gattcaggaa gtgctggagg ccagcgatat
     4921 tccgtatcgt catgaccgcc agttgttgca tagcgcgctg gaaaaggcat tacaggcttt
     4981 aggttaacac gaagccggaa gaatattccg gcttcgtcat cttgtcacga cgcggcaata
     5041 aacagctcac gcaactgatg caactggtca cgaatttgcg ccgcttcttc gaactccaga
     5101 ttctgcgcgt gttgcatcat caacccttcc agttcatgga tcttctgctg taacgcttta
     5161 ggcgacatat ccatcggcac attatccggt tcaacaatcg ggcgcgattt tcctctgccc
     5221 ttcgctttgg ttttggcaat gttctgcccc agcgccagga tatcgaccac tttcttgttc
     5281 aagccttgcg gcgtaatgcc gtgttcttcg ttgtacttct gctgtttctc gcggcgacgt
     5341 tcggtttcgc caatcgcttt cgccattgat ggggtgatct tatcgccata gagaatcgct
     5401 ttaccgttaa cgttacgtgc cgcacgacca atggtctgga tcaacgaacg ttcggaacgc
     5461 aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg aaacttccgg catatccaga
     5521 ccttcgcgca gtaagttgat ccctaccaat acgtcgaact cacccagacg caagtcgcgg
     5581 ataatctcca tacgttcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcaccg
     5641 tgttcttcga gatattcagt gagatcttcc gccatccgct tggtcagagt tgtaaccagt
     5701 acgcgttcgt taattgccgc tcgctgacga atctccgata gaagatcatc cacctgtgtc
     5761 gctaccggcc gcacttcgat aatcgggtcg agtaagcctg ttggacgcac cacctgatcc
     5821 accacatcgc caccggattt ttccagctcg taattacccg gcgtcgccga aacatagatg
     5881 gtttgcggcg ctaatgcttc gaactcttca aatttcagcg gacggttatc cagcgctgat
     5941 ggcaggcgga agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg
     6001 ccgccaattt gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcagcaggc
     6061 aggtaatcaa acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag
     6121 tagttctcaa tccccgaaca gtagcctagc tcgttcatca tctccagatc aaactgggta
     6181 cgctgggtca gccgctgctc ttccagcagt ttgttgtttt ccaatagcac tttacgtctg
     6241 gcggccagct cttctttgat ctcctccatc gcctggacga tgcgctcgcg tggcgtgacg
     6301 tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg aaacaatctg cccggtcagc
     6361 gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca gctccacgcg aagtgcaatg
     6421 tcatccgatt ctgccgggaa gatatcaatc acctcgccac gaacacggaa attaccacgc
     6481 tggaatgctt gatcattacg agcatattgc agctccgcca gtcggcgcag gatcgcgcgc
     6541 tgatcgataa tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg
     6601 cccagaccat aaatcgcgga aacagacgcc accacaacca catcacgccg ctccagcatc
     6661 gctttggtgg cggacaaacg catctgttcg atatgttcgt ttaccgaggc atctttctca
     6721 atgaaggtgt cggaactcgg tacataggcc tccggctgat agtagtcgta gtaggagacg
     6781 aaatattcca ccgcgttttc cggaaagaat tctttcattt cgccatacag ctgggccgcc
     6841 agcgttttgt tgggcgcaag taccatggtt gggcgctgaa ggtcagcaat gacattggca
     6901 atggtgaagg ttttcccgga gccagtcacg ccaagtaacg tctggtgcgc cagaccatct
     6961 tccagcccct cttcgagacg tcgaatcgcc tccggctgat cgccagaagg tttaaaagcg
     7021 gaattcagtt tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta
     7081 tgtaatttta ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt
     7141 catcaccata atatttctgt tacagcgtaa actccgctgt caatcatgaa caagatttac
     7201 tctgcggcga gataaaactc cggccttacc gggttatccc caaagcaacg gcttttttaa
     7261 catttgtcaa gatgattgat gacagtttta tggcaagaga tgcctgttca gtgactgcca
     7321 ttgcaattat ctaacctgtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca
     7381 atttgcgtgc aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat
     7441 ggttatccac aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa
     7501 ttcccacatt cactgcatga tgcggaggta aaattttttt cgtgattttt tagaatttaa
     7561 ttggttaaat tgcagtcaat cggagacgcg atctcgctca caatttaacc aagcacagga
     7621 tgactacatt aaggcaagat tgatgtactt tccggttgcc gcattttctg gattttctgc
     7681 aagccaggga atttcgccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     7741 ttcagcgtga cgttttcccg gaggcgtgac atcgttcgcc acccaccccg ccagagtcag
     7801 tccggcgtgt agtattgcct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     7861 gcccacaacc agtatcactg gcagttgttc ctgtgttacc caatccgcaa aagtgaaagt
     7921 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     7981 ttcaagcgcg cgtaatccgg cgctcattac caatgcttct atcggcctgc cctcttgcgc
     8041 gctgatgata tgcggcgagg tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8101 cagctgcagg ctactgttgc gctgtaacgc cagcgcgtca ctattacgta agcctttcgg
     8161 ggtcttttcg ctgccagagg ccaccggttt gtaacctgcc gtccggtagc ctgctgcctt
     8221 tgcggcttgt aaaagcgcgc agctggcgac agttttcccc acttcggtat ccgttccggt
     8281 gacaaaataa catttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8341 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8401 ttcgcgggtc acgcccttca tgaatatgcg tggcaccgat gcctttcagc gaacgcatgg
     8461 cactgagcgc atcatcaaac cacagcgtga tgggctgaat ataatgttga taatgaacgg
     8521 cgtttagcga ctgttcgatt ttatctggcg gtaaaaagcg attagcatgc gggcgctcgt
     8581 ccaccgcctg ccacgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8641 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccatg gataaattac
     8701 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     8761 gggattcgat atctcccgcc agataatggt ctgcggcatc cttctggcgt gcctgaacaa
     8821 gcattggcgg agagagatct aacgcagtca cctgcgcgtg acgctctcgc cagcggcggc
     8881 tcatccagcc tggtccacaa cccgcatcca gtacgcgggt gtatttacgc tgtgggagca
     8941 ttgccagtaa ggcgtcagca ctctggcgct gtagatcggc gtgttgctca tagtgtgcgg
     9001 ctgcccgtcc aaatgccgca gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9061 agcagacggt cgatatcctg catttcatgc gctgcggtta gcgttaagcg cagtcgcgca
     9121 gtaccagcgg gtacggttgg tgggcgaatt gccgtgaccc aacagccttg ctggcgcagt
     9181 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9241 catgagtcag caagcgtaaa cggcaaatcc tgcactccgg cacgaaaacg agtaatgagt
     9301 gccgccagtt tttcgcgccg tgcatccccc tcatcactgc gaatgaccgc cagcgacgca
     9361 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataagatg gcgagcgaat
     9421 tgcagcagat aatccgccac ggtatcgtag caaagcaccg ctgccccgct gacgccaaat
     9481 cctttgccaa acgtcaccac cagcaattct ggttttacct tttgcagcca gcagctgcca
     9541 cgcccctgct ccccgataac gcccgtgccg tgggcgtcat cgaccatcaa ccagccatcg
     9601 tgctgttgcg ttacttgctg gatttcctcc agtggcgcac tatcgccgtc catgctgaac
     9661 acaccttctg tcactaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
     9721 tgagtgacat cgttatgagc aaaacggcga agcggcgacg ggcttaaact tgcagcctcc
     9781 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
     9841 gcaatgacag cctgattagc ggcaaaacca gaaataaaca gcagtgcccg cgaatagccc
     9901 agccattcgg ccagttcttc ttccagcacc tggtgcgcca cgctataacc gctgacgtga
     9961 ccggaaccgc cgctaccgac accaaattgt tccgccccct gctgccaggc gcggataatt
    10021 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcaatca
    10081 tctgccacca gccagcgtcc ggctccttgt gccaccggat aacggcgacg caaggcatca
    10141 gcaacacgcc gcgcatcaag cgccgcgtcg attttctcct gccagatcat agtgctgccg
    10201 cgttgtaata ttcgtcagtg tccggggtca tcagcgcctg ctccagacgc tgctgttgtt
    10261 cgttatcgcc cgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10321 gcaggtcttt atcttcttcc ggattcggcg tagtcagcag tttgcaaccg tagaaaatcg
    10381 agtttgcgcc agccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10441 cagaaaggcg tacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtacggataa
    10501 aatcaaaggc atcgacatcg tcgttatccg ccagcggcgt gcctttcacc ttcaccagca
    10561 tgttgattgg cacgctttcc ggcggcgtcg gcaagtttgc cagttgcagc aataatccgg
    10621 cgcgatcttt caccgtttcg cctaagccca caatgccacc tgaacagact ttaatcccag
    10681 cttctcgcac tttttccagc gtatcaagac gttcctgata ggtgcgggtg gtgatgatat
    10741 tgccgtaaaa ctccggcgag gtgtcgaggt tgtggttata gtaatccagc ccggcattcg
    10801 ccaggcgctg cgcttgagat tcactcaacg tgcccagcgt catacacgcc tccagaccca
    10861 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    10921 tccacgccgc gcccatacag aaacgcgtcg atcccgccgc tttcgctttg cgcgccgact
    10981 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11041 tttgcgggca atatttgcaa tcttccggac aagctccggt cttaatcgac agcaaagtgc
    11101 tgacctgcac ctgacgagga tcgaaatgct gacgatgcac ctgctgcgct tcaaacagca
    11161 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11221 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag actcgtaaac
    11281 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tacctttgac
    11341 caacgccata tctggcaccc atacacatcc atgacctccc cgctgccggt ttatccggtg
    11401 gcaagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11461 tcgtcctggt gggcggcgat ccacggttac aatcacccgc agcttaatgc ggcgatgaag
    11521 tcgcaaattg atgctatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatt
    11581 gagctgtgcc gcaaattggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11641 gactccggct ccgtagcggt agaagtggcg atgaaaatgg cgttgcagta ctggcaggcc
    11701 aaaggcgaag cgcgccagcg ttttctaacc ttccgcaatg gttatcatgg cgataccttt
    11761 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    11821 ccagaaaacc tgtttgctcc cgccccgcaa agccgcatgg atggcgaatg ggatgagcgt
    11881 gatatggtgg gctttgcccg cctgatggca gcgcatcgtc atgaaatcgc ggcggtgatc
    11941 attgaaccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12001 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12061 ggatttggtc gtaccggcaa actgtttgcc tgtgaacatg cagaaatcgc gccggatatt
    12121 ttgtgtctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac gctcaccacg
    12181 cgcgaggttg cggaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12241 tttatgggca atccgctggc ctgcgcagca gcaaacgcca gcctggcgat tcttgaatct
    12301 ggcgactggc agcatcaggt ggcggctatt gaagcgcagc tacgcgagca acttgccccc
    12361 gcctgtgacg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg cgtggtcgaa
    12421 accactcgtc cggtgaatat ggcagcgctg caaaaattct ttgtcgaaca gggtgtctgg
    12481 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12541 ttgcagcgtc tgaccgcagc ggttaaccgt gcggtgcagg atgaaacatt tttttgccaa
    12601 taacgggaag tcagcgtgag ggtttctggc tacactttct gcaaacaaga aaggagggct
    12661 cttgacggct ccccgccctg aatgacgggg attcccactt caacaagccg aacctaagcc
    12721 gcgtgctgcg attcagggtt tacaggctct ccgtgggcta acactgccag cccggcagct
    12781 ttaatattac gtgccgcgtt aatgtcgcga tcatggtctg cgccgcattc agggcagtgc
    12841 catttacgaa cattaagagg cattttttgc atggtgaaac cgcagcaact acagcatttt
    12901 gaggacggta aatactggtc aatggcgacc actgaccgcc cggcccattt gcctttgtac
    12961 tggagctggc gaacaagttc gctccagcct gcgtcagcta ttgctttaga cagcttcggg
    13021 ttgcggatca tgtttttcac cttgagggat tcgacgcaaa caacttggtt ttcgttaatc
    13081 agtttgcggg acaacttgtg cagattgtcc atccggcaat cggcgatttt cgcgtggagt
    13141 cgggcgacct ttaagcgggc tttaattcgg tttcttgagc ctttttgctt cctgcttaaa
    13201 cgtcgctgta gcagcgttaa tcgcttcgca tatttagcgg tgtggcgggg attgtcggtt
    13261 ttgaatccgg tatcggtgac gaataaatct tttaagccca catcaatgcc gaccgtttta
    13321 gcggtaacag gcattgatac aggttcaaac tcacacaggc aggaaacaaa gtacctgcca
    13381 gcgctatctc tggaaatggt aacggttgac ggcgcagatg gtaattctcg actccagcga
    13441 acatccagcg gcgacttgct ctttgctata tacaactcgc cgtcacggtg tttaaacgcg
    13501 ctggcagtga actcagccac ctgtttgtgc cgtttgcttt tgaaagccgg atatgcagct
    13561 cgtccggcaa agaagttagc aaaggcggct tgttggtggc gcaacgactg ctggagggga
    13621 acgcaggaaa catcattcag ccatatgtat tcaggctctt ttttgagcgc cgtaaggcga
    13681 gcgttggcct gtagataacc gatcttttct tttcgctcgt agtacgcatc ggtacgccaa
    13741 cgaaggatgg aattgtagac gaagcgcaca cagccaaacg tctgagctaa aagctcagcc
    13801 tgctcagttg tcgggtaaaa ccggtattta tatgcgcgtt tcatgtgttc acatactaaa
    13861 gaggaaaatg tgattatgca aagtacagtt agtcggaaaa ccgcctcctt tcctccccgg
    13921 tctgaaggcc gaggtttccc ggaggcattc taatgaaact catcagtaac gatctgcgcg
    13981 atggcgataa gctgccgcat cgtcatgtct ttaacggcat gggttacgat ggcgataata
    14041 tttcaccgca tctggcgtgg gatgatgttc ctgcgggaac gaaaagtttt gttgtcacct
    14101 gctatgaccc ggatgcgcca accggctccg gctggtggca ctgggtagtt gttaatttac
    14161 ccgctgatac ccgcgtatta ccgcaagggt ttggctctgg tctggtagct atgccagacg
    14221 gcgttttgca gacgcgtacc gactttggta aaaccgggta cgatggcgca gcgccgccga
    14281 aaggcgaaac ccatcgctac atttttaccg ttcacgcgct ggatgtagaa cgtattgatg
    14341 tcgatgaagg tgccagcggc gcgatggtcg ggtttaacgt tcattttcac tctctggcga
    14401 gtgcctcgat tactgcgatg tttagttaat cactctgcca gatggcgcaa tgccatctgg
    14461 tatcacttaa aggtattaaa aacaactttt tgtcttttta ccttcccgtt tcgctcaagt
    14521 tagtataaaa aagcaggctt caacggattc atttttctat ttcatagccc ggagcaacct
    14581 gtgaacacat tttcagtttc ccgtctggcg ctgacattgg cttttggcgt gacgctgacc
    14641 gcctgtagct caacaccgcc cgatcaacgt ccttctgatc aaaccgcgcc tggtacctct
    14701 tctcgcccga ttctgtcggc aaaagaagcg cagaatttcg atgctcaaca ctattttgca
    14761 tccctgacac caggtgcggc agcgtggaat ccttccccga ttaccctgcc tgcgcaacct
    14821 gactttgttg tcggcccggc gggtactcaa ggtgtaacgc ataccacgat tcaggcggcg
    14881 gtagatgcgg caattatcaa gcgtaccaac aagcgccagt atattgccgt gatgcctggt
    14941 gagtatcagg gaacggtgta tgtccctgcc gctccgggtg gaattactct atacggtaca
    15001 ggtgaaaaac cgattgatgt gaagattggg ctttcccttg atggtggcat gagccctgcc
    15061 gactggcgtc acgacgtcaa cccgcgcggc aaatatatgc caggtaaacc ggcgtggtat
    15121 atgtacgata gctgccagag taaacgcagc gacagtatcg gtgttctctg ctctgcggtc
    15181 ttctggtcac aaaacaatgg cctgcaactg caaaacctga ccatcgaaaa cacgctgggc
    15241 gatagcgtag atgcgggtaa ccatccggcg gtggcactgc gtactgatgg cgacaaagtg
    15301 cagatcaata acgtcaacat tctcggtcgt cagaacacct tctttgtcac caacagcggt
    15361 gtgcagaacc gtctggaaac gaatcgtcag ccgcgtacgc tggtgaccaa cagctatatt
    15421 gaaggggatg tggatatcgt ttctggtcgc ggcgcagtgg tgttcgataa caccgaattc
    15481 cgcgtggtga actcccgtac ccagcaagaa gcgtatgtgt ttgcaccggc tacgctgtcc
    15541 aacatttact acggtttcct cgccgtaaac agccgtttca atgcttccgg tgatggcgtg
    15601 gcgcaactgg gccgctcgct ggatgttgat gccaatacca acggtcaggt agtgatccgt
    15661 gatagcgcca tcaacgaagg ttttaacaca gccaaaccgt gggctgatgc ggtgatctct
    15721 aatcgtccat ttgcgggtaa caccggcagc gttgatgata acgacgaagt acagcgcaat
    15781 ctgaatgaca ctaactacaa ccgcatgtgg gaatacaata accgcggcgt gggtagcaaa
    15841 gtggttgcag aggcgaagaa gtaggagctg ttaactatgt gccggatgcg gcgtaaacgc
    15901 cttatccggc ctacagttcg atacgatttg tcggtcggat aagatgcgcc agcatcgcat
    15961 ccgacaataa gtgccggatg cgacctacat tcacatggcg ctttttacat ctgacggttt
    16021 ttattgaagt taatcaaact acctgccttg ataatctcgc gctcttcagc agtcagactt
    16081 tccatataga gcgtaatctc cgttaccggt gcatcttcat ggatcacata acctttaaac
    16141 gtcgttccag gattatccag cgccgcttta atgccaggga tataaatgta atcacctacg
    16201 tcaaagttcg gtacttccgc catctgcagc ggtaacatcc cccagttgat gacgttagaa
    16261 cgatagcgtt tagtcgcgta ctcctcggca atattcgcca gaccgccaat cacacgctga
    16321 caactcgccg cctgttcacg cgcagaacca tcgcctggtt tcaccgcata aaccatgctg
    16381 ccaatttcag tttgcagcgg atcaacatgt tcctgaccag caatctgctt aatgcgcgca
    16441 aacacatctg ccatctcgct gacatttccc gccagacgct gattttccag ctcggcagtc
    16501 gctttacttc tgccaacata acctggatcg cggcgtgaca gagtaaactc cgccagacca
    16561 atcggatttg aacgatatga agaggtttca ccggaaggaa tcagttcgtc ggtggtggtc
    16621 acttcgtcga ggatcttcga gcacactttg aggacgatat tgtcagtcag cgcacccaat
    16681 tctggccagt ctttaatgtt cggtccgtaa atcagcggtt gctgggtcgc ccctttcaca
    16741 aagccctgat aaacacggtt tttatacggc gttacatcga aggcgtactc cggcacgttg
    16801 tcccagcaat cgagttcgct ggcagaggtt aaatagccac cgtttgccgc agtcgcagcg
    16861 atagaacgag cgtccatcaa cgccaccgct gacatctgcc cattagctgg cttagagcct
    16921 tcgcggttcg ggaagttacg cgtggtgtgg cgaatactca aaccgttgtt gattggcgta
    16981 tcgcccgcgc caaagcatgg gccgcagaac gcggttctga tgattgcgcc tgcgccgatc
    17041 aaatctgcta ccacaccttt tttggcgaga tccataaaca ccggctgtga tgacgggtaa
    17101 accgctaacg agaaggtgtc attgccgcag gattgaccgc gcagcgcatt cgccgccgcg
    17161 atgacgtttt cgtagttacc gccagaacag cccgcgataa tcccctgctg cactttcagg
    17221 cgaccatttt ccactttatc cagcagcgag agtttggctt taccgtgcgc cacgcgttcg
    17281 gactcaattt caatctcacg cagaatgtcg gtcaggttct ggttcagcgt gtcgatttca
    17341 tacacgttgc tcgggtggaa tggcagcgca atcattggtt tgatggcgct taaatcaacg
    17401 ctgatgcagc catcgtagta cgccattggt tgagggttaa gttggcagta atcctggccg
    17461 cgaccgtgca gcgccagcca gttatggact tcttcatcgg tttgccagac agaacttaaa
    17521 caggtcgttt cagtggtcat cacgtcaacg ctgttacgga aatcggtaga gagcgctgca
    17581 acaccgggac ctacgaactc catcactttg tttttgacgt aaccgttttt gaacacagca
    17641 ccgatgatag ccagcgccac atcctgcggc cccacatacg gcgcaggttt tccggtcaga
    17701 tgcaccgcaa ccacgcccgg atagtcgata tcccaggtgt cattaagcag ctgttttaac
    17761 aactcgccgc cgccctcgcc aaccgccatt gtccctaacg caccgtaacg ggtatggctg
    17821 tctgacccga ggatcatttt gccgccgccc gccatcattt cacgcatata ttgatggatg
    17881 accgcaatat gcggtggcac aaaaataccg ccataacgct gggccgccga taaaccaaaa
    17941 acatggtcat caccgttaat ggtgccgccc acagcacaga gtgagttatg gcagttggtc
    18001 agcacatacg gcagcgggaa acgttccata ccggacgctt tagcggtctg tacaatacca
    18061 acaaaggtaa tatcgtgaga ggctaatgaa tcaaacttaa ttttaagttt atccatattt
    18121 ccggacgtat tatgagaaga gagaatagac caggcaatag tgcctttttt tgcttcttct
    18181 ttttttattt cgccggtgaa atgttcttcg gcaattattt cgttattgct ggcgagaaac
    18241 acgccttttt cagataactt gatcatcaaa tgctccagat atctgcgcac cgtgaagtgc
    18301 gcagaaagat aataaatgga tatactctaa ataattcgag ttgcaggaag gcgacaagcg
    18361 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
    18421 catgcaactt gaagtatgac gagtatattt acagcatgtt ccagccgatc agcatattcc
    18481 accaccacac gccgagggtg atatgcacca ggaaggttaa tatcgtcagt accgcaccga
    18541 ccaaccacca ggatttaata tcgttgtaac ccacgccaaa gatgaccgga cctgccgcgc
    18601 cgccatagtg agtaaccatg ccgccatagg agttagagaa caacagtgcc agcgcggtta
    18661 acattaacgg tgcgccggag acattcgcca gcatggcaaa cacaggtaac atagcaacga
    18721 tataggcact accagaagcg aagaaataac gcacgatgat gctgaggaaa ataataacga
    18781 agaaagcaac gttaccgtga ccatcaaatg ccaggttatt tttaaagact tcagctaacc
    18841 attcgaagaa tttaactttc gataataagg agcttaagcc gataataccg ccgtaccaga
    18901 ttaaggtatt ccagccgcct ttatttttaa ccacgtcttc ccaggtaacg atacccagca
    18961 gcagcatagt tgccataaca acgattgcca cggtggattc atcaaccccc agagacttac
    19021 tgaaaatcca gcccagcagc gccagcacaa agacaccgag cagcattttt tcgcggattt
    19081 tcatcggtcc cagttcggca aggcccgctt tagcgatggt tttgttatcc accttcttaa
    19141 tttctggtgg atacatggtg taaatcacca gcggggtgac cagcagcata atgatgcccg
    19201 gcaatccggc ggctaacgcc catccacccc agctaatttg caggtgcaga atgtcgttga
    19261 tcattttcag cgccagaatg ttccccgcca ttgcagtaaa gaacatatag ctggtggttt
    19321 tggtgaccat gtaaatggac atcatcaggt aatgtccaac acgacgcgga cttttttccg
    19381 gttctgatcc caaagccact gccacgctgt tgatgatcgg taacacgatg ccgcccgcac
    19441 gcgcggtgtt agacggtgtt gccggagcca gtaccagatc gagaaatacc gtaacgtaac
    19501 ccagacccag ggtagtgcta ccaattttac caatcagcag ataggcaata cgtttaccta
    19561 agcctgtggt tacaaacgcg gcgcttaaag taaacgcaga aaacaccagc caggtggtac
    19621 cggaagagta accgcttaat acggcggtgg ttttaaattc cccacctgat aagttaccca
    19681 ccactaccat cgatgcggcg acggcaatta acagtacgac aggttccggg aaaggcttga
    19741 taaccagccc cacaatggcc gccaggtaaa taccaaaaag cacccacgcc agttcgctta
    19801 atcctgccgg agctggcata aagccaataa tacatgggat cgctaatatc ag
//
LOCUS       DAZFRG010000074        19781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFRG010000074 DAZFRG010000000
VERSION     DAZFRG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.074"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            545..1735
                     /locus_tag="ACHKUC_002073"
     CDS             545..1735
                     /locus_tag="ACHKUC_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO6618328.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            1748..2266
                     /locus_tag="ACHKUC_002074"
     CDS             1748..2266
                     /locus_tag="ACHKUC_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6618329.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            2324..2599
                     /locus_tag="ACHKUC_002075"
     CDS             2324..2599
                     /locus_tag="ACHKUC_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6618330.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            2596..2751
                     /locus_tag="ACHKUC_002076"
     CDS             2596..2751
                     /locus_tag="ACHKUC_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6618331.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            2744..5191
                     /locus_tag="ACHKUC_002077"
     CDS             2744..5191
                     /locus_tag="ACHKUC_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6618332.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            5206..5685
                     /locus_tag="ACHKUC_002078"
     CDS             5206..5685
                     /locus_tag="ACHKUC_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618333.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMETRFTGMTGAG"
     gene            5685..6848
                     /locus_tag="ACHKUC_002079"
     CDS             5685..6848
                     /locus_tag="ACHKUC_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6618334.1"
                     /translation="MLDTLTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKKPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDD"
     gene            6929..7147
                     /gene="ogrK"
                     /locus_tag="ACHKUC_002080"
     CDS             6929..7147
                     /gene="ogrK"
                     /locus_tag="ACHKUC_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO6618335.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVGPHPLPSGQQIMWM"
     gene            7383..8285
                     /gene="fieF"
                     /locus_tag="ACHKUC_002081"
     CDS             7383..8285
                     /gene="fieF"
                     /locus_tag="ACHKUC_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO6618336.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            8466..9428
                     /gene="pfkA"
                     /locus_tag="ACHKUC_002082"
     CDS             8466..9428
                     /gene="pfkA"
                     /locus_tag="ACHKUC_002082"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO6618337.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            9748..10737
                     /gene="sbp"
                     /locus_tag="ACHKUC_002083"
     CDS             9748..10737
                     /gene="sbp"
                     /locus_tag="ACHKUC_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO6618338.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKDTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            10873..11598
                     /gene="cdh"
                     /locus_tag="ACHKUC_002084"
     CDS             10873..11598
                     /gene="cdh"
                     /locus_tag="ACHKUC_002084"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO6618339.1"
                     /translation="MIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLPNQQENQNPSP
                     CAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFWLAWQARDFMS
                     QKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLANISSRWLPLPG
                     GLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQSDNSFVLLATQ
                     RNLLTLNRASAEEIQDHQCEILR"
     gene            complement(11653..12420)
                     /gene="tpiA"
                     /locus_tag="ACHKUC_002085"
     CDS             complement(11653..12420)
                     /gene="tpiA"
                     /locus_tag="ACHKUC_002085"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO6618340.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(12528..13127)
                     /gene="yiiQ"
                     /locus_tag="ACHKUC_002086"
     CDS             complement(12528..13127)
                     /gene="yiiQ"
                     /locus_tag="ACHKUC_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HFO6618341.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            13228..13668
                     /gene="yiiR"
                     /locus_tag="ACHKUC_002087"
     CDS             13228..13668
                     /gene="yiiR"
                     /locus_tag="ACHKUC_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6618342.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            13880..14179
                     /gene="yiiS"
                     /locus_tag="ACHKUC_002088"
     CDS             13880..14179
                     /gene="yiiS"
                     /locus_tag="ACHKUC_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO6618343.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            14206..14634
                     /gene="uspD"
                     /locus_tag="ACHKUC_002089"
     CDS             14206..14634
                     /gene="uspD"
                     /locus_tag="ACHKUC_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO6618344.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(14639..15385)
                     /gene="fpr"
                     /locus_tag="ACHKUC_002090"
     CDS             complement(14639..15385)
                     /gene="fpr"
                     /locus_tag="ACHKUC_002090"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO6618345.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(15482..16492)
                     /gene="glpX"
                     /locus_tag="ACHKUC_002091"
     CDS             complement(15482..16492)
                     /gene="glpX"
                     /locus_tag="ACHKUC_002091"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6618346.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDIYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            complement(16663..18171)
                     /gene="glpK"
                     /locus_tag="ACHKUC_002092"
     CDS             complement(16663..18171)
                     /gene="glpK"
                     /locus_tag="ACHKUC_002092"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO6618347.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            complement(18194..19039)
                     /gene="glpF"
                     /locus_tag="ACHKUC_002093"
     CDS             complement(18194..19039)
                     /gene="glpF"
                     /locus_tag="ACHKUC_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO6618348.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            19464..19709
                     /gene="zapB"
                     /locus_tag="ACHKUC_002094"
     CDS             19464..19709
                     /gene="zapB"
                     /locus_tag="ACHKUC_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO6618349.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
BASE COUNT         4867 a         4892 c         5320 g         4702 t
ORIGIN      
        1 aaagtaatcc tttataaact cctcctgaag atcttggtct tttttgtagc tctctaggat
       61 tccagcaaaa agaaatgacc ctaaagatat agagattatg gatattagca attgactaag
      121 gaatgattgt gtttcattac tcataaaaca tccttaaaaa ataacacgaa aagtgactcc
      181 tgacagggaa gaaacgacag gtcggaaaca aacttaactg atgagttagc tgtagtccag
      241 aaccttgttt taacacttag tgtgactgtt tgcatctcag gaatttgtgc caataatatt
      301 tgaatcctta taatttattt aaatcaaatt tagttactcg ctcaaagcag gctgtcagat
      361 ttgatagcgt ttgggctatg taaactgtca gtcggaaaat gagtgtgtac aaatcaggac
      421 aggcgagctg attgcccgcc ttttctttat ctgttgtttc atccactgac cagccaggtc
      481 aaatagcgtc tcatgctctg cacaacagaa aatagttgca cccattaacc acggagttaa
      541 acggatgagt gactatcatc atggcgtgca ggtgctggag attaacgacg gcacccgcgt
      601 catttccacc gtatccactg ccattgtcgg catggtctgc acggccagcg atgcggatgc
      661 ggaaaccttc cctctcaata aaccggtgct gattaccaat gtgcagagcg caattgcaaa
      721 ggccggtaaa aaaggcacgc tggcggcgtc gttacaggcc atcgctgacc agtcaaaacc
      781 ggtcaccgtt gttgtgcgcg tggaagacgg caccggcgac gacgaggaaa cgaaactcgc
      841 gcagactgtt tccaatatta tcggcaccac cgacgaaaac ggtcagtaca ccggactgaa
      901 agccctgatg ggggctgagt cggttaccgg cgttaaaccg cgcattctcg gcgtaccggg
      961 actggacacc aaagaggtcg ccgtcgcact ggcatcggta tgccaggaac tgaatgcctt
     1021 cgggtatatc agcgcatggg gttgtaaaac catttccgag gtgaaagcct accgccagaa
     1081 tttcagccag cgtgagctga tggttatctg gccggatttc ctcgcatggg atacggttac
     1141 cagtaccacc gccaccgcgt atgccaccgc tcgtgcgctg ggtctgcgcg ctaaaatcga
     1201 ccaggagcag ggctggcata aaacgctgtc caacgtcggg gtgaacggtg ttaccggcat
     1261 cagtgcatcc gtcttctggg atttgcagaa gccaggcact gatgctgacc tgctgaacga
     1321 ggcaggcgtc acaacgctgg ttcgccgcga cggttttcgt ttctggggta accgtacctg
     1381 ctctgatgac ccgctgttcc tctttgaaag ctacacacgc accgcgcagg tactggccga
     1441 cacgatggct gaggcgcaca tgtgggctat tgataagccc atcaccgcaa cgctgattcg
     1501 cgacatcatt gacggcatca atgccaaatt ccgtgaactg aaaaacaacg gttatatcgt
     1561 ggatggcaca tgctggttca gcgaagaatc caacgatgcg gaaaccctca aggccggaaa
     1621 actgtatatc gactacgact ataccccggt gcctcctctc gaaaacctga ccctgcgcca
     1681 gcgtattact tccagatacc tggcaagtct ggtcacttcg gttaacagca attaaggagc
     1741 ctgaccgatg gcaatgccgc gcaaactcaa gttaatgaac gtctttctga acggctacag
     1801 ctatcagggc gttgcaaagt ccgtcacgct gccaaaactg acccgtaagc tcgaaaacta
     1861 tcgcggtgcg gggatgaacg gcagcgcacc ggtagacctc ggccttgatg atgatgctct
     1921 gtcaatggag tggtcgctcg gtggattccc ggattcggtt atctgggagc tttacgctgc
     1981 aaccagtgct gatgccgtac cgattcgttt tgctggttct taccagcgcg acgataccgg
     2041 cgaaacggtg gccgtcgaag tggtcatgcg tgggcgtcag aaagaagtcg ataccggaga
     2101 gggtaaacag ggagaggata ccgaatcgaa actcccggtg atttgtactt atttcaagtt
     2161 gacgatggac ggtaaggagc tggtcgaaat cgacaccatc aacatgattg agaaggtgaa
     2221 cggcgtcgac cggctggagc aacaccgccg caatatcggc ctgtgatttt catccgggtc
     2281 agcctggctg accggttaac cccgattcag aagtgagaaa accatgaaca aagaaaatgt
     2341 gattaccctg gacaatccgg tcaagcgtgg tgagcaggtt atcgaacagg tcacgctgat
     2401 gaaacctaat gccgggacgc tgcgcggtgt cagtctggct gcggtcgcaa actccgaagt
     2461 cgatgcactg attaaggtgc tgccgcgcat gacggcaccg atgctgaccg agcaggaggt
     2521 cgccgcgctg gaactgcctg accttgtggc gctggccggt aaggtggtcg gttttttgtc
     2581 tccgaactcg gtgcagtgac gtttccgaaa aatctgtcgg tcgatgacct gatggcagat
     2641 gtggcagtga tatttcactg gccgccatca gaactgtatc ccatgagcct gaccgaactc
     2701 atcacatggc gcgaaaaggc gctccggcga agcggaagca cgaatgagta acaatgtaaa
     2761 attacaggta ttgctcaggg ctgttgacca ggcatcccgc ccgtttaaat ccatccgtac
     2821 agcgagtaag tcgctgtcgg gggatatccg ggaaacacaa aaatcactgc gcgagctgaa
     2881 cggtcacgca tcccgtattg agggatttcg caagaccagc gcacagcttg ccgtgactgg
     2941 tcatgcactt gaaaaggcac ggcaggaagc cgaagcactt gccacacagt ttaaaaacac
     3001 tgaacgtccg acccgtgctc aggcgaaagt gctggaatcc gcaaagcgtg cggcggagga
     3061 cttacaggcg aaatataacc gcctgacgga ttccgttaag cgccagcagc gggaactggc
     3121 cgctgtggga attaataccc gcaatcttgc acatgatgag cagggactga aaaaccgtat
     3181 cagtgaaacc accgcacagc ttaaccggca gcgtgacgcg ctggcgcgtg tcagtgcgca
     3241 acaggcaaaa cttaatgcag taaaacagcg ttatcaggcc ggaaaggaac tggccggaaa
     3301 tatggcctca gtgggcgctg ccggtgtggg gattgcggcg gcgggaacga tggccggagt
     3361 taagttgctg atgcccggtt atgagtttgc gcagaaaaac tcagaattgc aggccgtgct
     3421 gggtgtggca aaagactccg ccgaaatgac cgcactacgc aaacaggcgc gccagctcgg
     3481 cgacaatacc gccgcctcgg cggatgatgc ggccggtgca cagataatca tcgcgaaagc
     3541 gggtggggat gttgatgcca ttcaggcggc aacgccggtc acgctgaata tggcgctggc
     3601 gaaccgccgc acgatggaag aaaacgccgc cctgctgatg gggatgaaat ccgcctttca
     3661 gctttcaaac gataaggtcg ctcatatcgg ggatgttctc tccatgacga tgaacaaaac
     3721 cgccgccgat tttgacggca tgagcgatgc gctgacctat gccgcacctg tggcaaaaaa
     3781 tgccggtgtc agcattgaag aaaccgccgc aatggtcggg gcgctgcatg atgcaaaaat
     3841 cacaggctca atggcgggga cgggaagccg tgccgtgtta agccgcctgc aggcaccgac
     3901 gggaaaagca tgggatgcac tcaaagagct tggagtgaaa acctcagaca gcaagggaaa
     3961 cacccggcca atatttacca ttctgaaaga aatgcaggcc agttttgaga aaaaccggct
     4021 cggtactgcc cagcaggctg aatacatgaa aaccattttc ggggaggagg ccagctcagc
     4081 cgccgccgtg ctgatgactg ccgcctcaac cggaaagctg gacaaactga ccgctgcgtt
     4141 taaagcctcg gacgggaaga cctcagagct ggtaaatatt atgcaggaca atctcggcgg
     4201 tgactttaag gagtttcagt ccgcttatga ggcggtgggg actgacctgt ttgaccagca
     4261 ggaaggcgca ctgcgtaagc tcacgcagac ggccacaaag tatgtgttaa aactcgacgg
     4321 ctggatacag aaaaacaaat cactggcgtc aaccatcggc atcattgccg gcggtgcact
     4381 ggtgctgact ggcatcatcg gtgccattgg ccttgtagct tggccggtta tcaccggcat
     4441 caatgccatt atcgcggcag caggcgcaat gggggcaatc ttcacgacgg ttggtagtgc
     4501 cgttatgacg gccatcggtg cgattagctg gccggttgtg gccgtggtgg ccgctattgt
     4561 cgccggggcg ttacttatcc gtaaatactg ggagcctgtc agcgcattct ttggcggtgt
     4621 ggttgaaggg ctgaaagcgg catttgcgcc ggtgggggaa ctgttcacgc cacttaagcc
     4681 gctgtttgac tggctgggtg aaaagttaca ggccgcgtgg cagtggttta aaaacctgat
     4741 tgccccggtt aaagccaccc aggacacact gaaccgttgc cgtgacacgg gcgtcatgtt
     4801 cgggcaggca ctggctgacg cgctgatgct gccgcttaat gcgttcaaca aactgcgcag
     4861 cggtattgac tgggtactgg aaaaactcgg tgttatcaac aaagagtcag acacacttga
     4921 ccagaccgcc gcaaggactc atgccgccac gtatggcacc ggtggttata ttccggcgac
     4981 cagctcttat gcaggctatc aggcttatca gccagtcacg gcaccggctg gccgctctta
     5041 tgtggaccag agtaaaaacg aatatcacat cagcctgacg ggtggtactg cgccggggac
     5101 acagcttgac cgccagttac aggatgcgct cgaaaaatac gagcgggata aacgtgcgcg
     5161 cgcccgtgcc agcatgatgc atgacggtta aggaggtgac gaaaaatgat gctcgcgtta
     5221 ggtatgtttg tttttatgcg ccagacgctg ccacaccaga ccatgcagcg tgaatcggat
     5281 tatcgctggc cgtcaaattc ccgtatcggt aaacgggatg cctttcagtt tctcggtgtg
     5341 ggtgaggaaa acatcacgct ggccggtgtg ctttatcccg aactgaccgg cggaaagctg
     5401 acgatgacca cgctcagact gatggcagag gaaggtcggg cgtggccgtt gctggatggc
     5461 accggcatga tttacggcat gtatgtcatc agcagggtga gtgaaacagg gagtattttc
     5521 tttgcagacg gcacaccccg gaaaattgat tttacgctgt cgctcacccg cgttgatgaa
     5581 tcactggccg cgctttatgg cgatatcggt aaacaggcgg aatcgctcat cggtaaggct
     5641 ggcagtatgg agaccagatt cacgggtatg acgggggcgg gataatgctg gatacgctga
     5701 catttgatgc aggcagtacg ctgacgccgg attacatgct gatgctcgac agcagggata
     5761 ttaccggcaa tatcagcgat cgactgatga gcatgaccct gacggataac cggggctttg
     5821 aggctgacca gcttgatatt gaactgaacg atgccgacgg gcaggtcggg ctgccggttc
     5881 gtggcgctgt cctgacggtg tatatcggct ggaaaggttt tgccctggta tgcaaaggga
     5941 aatttaccgt tgatgaggtt gaacaccggg gcgcaccgga tgtggtcacc atccgcgccc
     6001 ggagtgcaga ttttcgcggg acgctcaatt cccgccgtga aggctcctgg catgacacca
     6061 cgctcggtgc gattgttgag gcgatagcct cccgtaacag gctggaagcc agtgtcgctc
     6121 cgtcactggc cggaattaaa atcccgcaca tcgaccagtc gcaggagtct gatgcgaaat
     6181 tcctgacccg tcttgcagaa cgcaacggcg gtgaggtgtc ggtaaaaatg ggaaaactgt
     6241 tgtttctcaa agcggggcag ggggtgacgg ccagcggtaa aaaaaaaccg caggtcacca
     6301 taacccgcag cgacggcgac cgccatcatt ttgcgattgc tgaccgtgga gcctataccg
     6361 gcgtaacggc aaagtggtta cacaccaaag acccgaagcc acaaaagcag aaggtaaaac
     6421 tgaaacgcaa aaagaaagag aaacacctgc gcgcactgga gcacccgaaa gcgaaaccag
     6481 tcacgcagaa gaaagcgcca aaagtaccgg aagcgcgcga aggtgaatac atggctggtg
     6541 aggctgacaa cgtttttgcc ctgaccacgg tatatgccac gaaagcgcag gccatgcgcg
     6601 ccgctcaggc gaagtgggat aaactgcaac ggggcgttgc ggagttctcc atcagccttg
     6661 ccactggtcg tgctgatatt tacacggaaa caccggtcag agtgtcaggc tttaagcgcg
     6721 tcatagacga gcaggactgg acaatcacta aggtgacaca ttttctgaat aatagtggct
     6781 tcacgacgtc cttagagctt gaggtcaggc tttctgatgt ggagtacgaa acagaagatg
     6841 atgattaatg ttttgtttta tctgtttgtt ttataaggat aaattaaata aaatggcacc
     6901 attaacaaaa ccggaagagg tgctcgcgat gtttcattgt cctttatgcc agcatgccgc
     6961 acatgcgcgt acaagccgct atatcactga cacgacaaaa gagcgttatc accagtgcca
     7021 gaacgtgaat tgcagcgcca cgttcatcac ttatgagtcg gtacagagat acatcgtgaa
     7081 gccgggagaa gtccacgccg tggggccgca cccgttgccg tcagggcagc aaattatgtg
     7141 gatgtgatca caaaaatagc ccctcagtcg aggggcttat ttatggtcga tgtggacgct
     7201 atgtggacag tggttgacat aaatccattt atatcatcag gttaggtgct tttttgtgac
     7261 accatccctg tcttccccca catgatgtgg gggttttttt tatcctcaat ttgcctgctg
     7321 cttaatgcat tgcagatgat ttgcttccgt tatactaccg tcagttgata gcgggagtat
     7381 ttatgaatca atcttatgga cggctggtca gtcgggcggc gattgctgcg acggcgatgg
     7441 cttcgctgct attgctgatt aaaatttttg catggtggta taccgggtcg gtgagtattc
     7501 tcgccgcgct ggtggattcg ctggtggata tcggcgcgtc gttgacgaat ttactggtgg
     7561 tgcgatattc cctgcaacct gccgacgata atcactcgtt tggtcacggt aaagctgagt
     7621 ccctcgcggc gctggcgcaa agtatgttta tctccggttc ggcactattc ctgtttttga
     7681 cgggtattca acatctggta tctccaacac cgatgacaga tccaggcgtc ggggttatcg
     7741 tgacaattgt ggcgctaatt tgtacgatta tccttgtctc gtttcagcgt tgggtggtgc
     7801 gccggacgca aagccaggcg gtgcgggctg atatgctaca ttaccagtct gatgttatga
     7861 tgaacggcgc aattctgctg gcgctggggt tgtcctggta cggctggcat cgcgccgatg
     7921 ctctgtttgc attgggaatc ggcatctata ttttatatag cgcgttacgc atgggatatg
     7981 aggcggtaca gtcattactg gatcgcgcat tacctgatga ggaacggcaa gaaattattg
     8041 atatcgtgac ttcctggccg ggtgttagcg gcgctcacga tcttcgcacg cggcagtcag
     8101 ggccgacccg ctttattcag attcatttgg aaatggaaga ctctctgcct ttggttcagg
     8161 cacatatggt ggcggatcag gtagagcagg ctattttacg gcgttttccg ggatcggatg
     8221 tgattatcca tcaggacccc tgttccgtcg tacccaggga gggtaaacgg tctatgcttt
     8281 cataatcagt ataaaagaga gccagacccg cattttgtgt ataaaatacc gccatttggc
     8341 ctgacctgaa tcaattcagc aggaagtgat tgttatacta tttgcacatt cgttggatca
     8401 tttcgatgtg caagaagact tccggcaaca gatttcattt tgcattccaa agttcagagg
     8461 tagtcatgat taagaaaatc ggtgtgttga caagcggcgg tgatgcgcca ggcatgaacg
     8521 ccgcaattcg cggggttgtt cgttctgcgc tgacagaagg tctggaagta atgggcattt
     8581 atgacggcta tctgggtctg tatgaagacc gtatggtaca gctagaccgt tacagcgttt
     8641 ctgacatgat caaccgtggt ggtacgttcc tcggttctgc gcgtttcccg gaattccgcg
     8701 acgagaacat ccgcgccgtg gctatcgaaa acctgaaaaa acgtgggatc gacgcgctgg
     8761 tggttatcgg cggtgacggt tcctacatgg gtgcaatgcg tctgaccgaa atgggcttcc
     8821 cgtgcatcgg cctgccgggc actatcgaca acgacatcaa aggcactgac tacactatcg
     8881 gtttcttcac tgcgctgagc accgttgtag aagcgatcga ccgtctgcgt gacacctctt
     8941 cttctcacca gcgtatttcc gtggtggaag tgatgggccg ttattgtggc gatctgacgt
     9001 tggctgcggc cattgccggt ggctgtgaat tcgttgtggt tccggaagtt gaattcagcc
     9061 gtgaagacct ggtaaacgaa atcaaagcgg gtatcgcgaa aggtaaaaaa cacgcgatcg
     9121 tggcgattac cgaacatatg tgtgatgttg acgaactggc gcatttcatc gagaaagaaa
     9181 ccggtcgtga aacccgcgca actgtgctgg gccacatcca gcgcggtggt tctccggtgc
     9241 cttacgaccg tattctggct tcccgtatgg gcgcttacgc tatcgagctg ctgctggcag
     9301 gttacggtgg tcgttgcgta ggtatccaga acgaacagct ggttcaccac gacatcatcg
     9361 acgctatcga aaacatgaag cgtccgttca aaggcgactg gctggactgc gcgaaaaaac
     9421 tgtattaatg atttcggaaa aaggcagatt cctttagcct gaaaccgatg acagaagcaa
     9481 aaaggcctga tgcgcttcgc ttatcaggcc tacatgaatt ctgtaatcta ttgaatttgc
     9541 aaacttttgt aggccggata aggcgtttgt gccgcatccg gcatggacaa agcgcacttt
     9601 gtcagcaata tgaggcggat ttcttccgcc tttttaatcc cccaacatat actcgcaagt
     9661 tatagccaat ctttttttat tctttaatgt ttggtcaacc ttctggcacg ctttgctcat
     9721 cacaacacaa cataagagag tcgggcgatg aaaaagtggg gcgtagggtt aacatttttg
     9781 ctggcggcaa ccagcgttat ggcaaaggat attcagcttc ttaacgtttc atatgatcca
     9841 acgcgcgaat tgtacgaaca gtacaacaag gcattcagcg cccactggaa acagcaaact
     9901 ggcgataacg tggtgattcg tcagtcacac ggtggttcag gcaaacaagc gacgtcggta
     9961 atcaacggta ttgaagctga tgttgtcacg ctggctctgg cctatgacgt ggacgcaatt
    10021 gcggaacgcg ggcggattga taaagagtgg atcaaacgtc tgccggataa ttccgcaccg
    10081 tacacttcca ccattgtttt cctggtacgt aagggtaatc cgaagcagat ccatgactgg
    10141 aacgatctga ttaaaccggg tgtttcggtg atcacgccta atccgaaaag ctctggtggc
    10201 gcgcgctgga attacctggc agcctggggc tacgcgctgc atcacaacaa caacgatcag
    10261 gcaaaagcac aggattttgt tcgggcactg tataaaaacg tcgaagttct ggattctggc
    10321 gcgcgcggat ccactaacac ttttgtcgag cgcggaattg gcgatgtact gattgcctgg
    10381 gaaaatgaag ctctgctggc agcgaatgaa ctggggaaag ataaattcga aatcgtcacg
    10441 ccgagtgagt ctatcctcgc agagccaacc gtgtcggtgg tcgataaagt ggtcgagaaa
    10501 aaagacacca aagaggtggc ggaagcctac ctgaaatatc tctactcgcc agaaggtcag
    10561 gaaattgcag cgaaaaacta ctaccgtccg cgcgacgctg aggtagcgaa aaagtacgaa
    10621 aatgcgtttc caaaattgaa gttattcacc attgatgaag agttcggcgg ctggacgaaa
    10681 gcgcaaaaag agcattttgc taacggcggt acgttcgatc agatcagcaa acgctgattt
    10741 ccccaggata attatcaagc ccggtggttt ctcgccactg ggttttttat ttgtcacgat
    10801 tttgcgttac ccttgcatct ctttgaggta cagggaaaaa aagatgaaaa aagcgggtct
    10861 tcttttttgg tgatgatagt tatcgccgtt gtggctgccg gtattggtta ctggaaatta
    10921 accggtgaag agtcagacac gttacgtaag attgttcttg agcaatgttt gcccaaccag
    10981 caggaaaacc aaaacccgtc gccatgtgcg gaagtcaaac ccaacgccgg atacgtggtt
    11041 ttaaaagatc gtcatggccc actgcaatat ctgttgatgc caacgtaccg tattaacggt
    11101 actgaaagcc cattgttgac cgatccgtca acgcctaatt ttttctggtt agcctggcag
    11161 gcgcgtgatt ttatgagcca aaaatacggc cagcctgttc ccgatcgcgc ggtatctctg
    11221 gcgatcaact cccgcaccgg gcgtacgcaa aaccattttc atattcatat ttcttgtatt
    11281 cgtcctgatg tgcgcgaaca gctggataac aatctggcga acatcagtag ccgctggttg
    11341 ccactgccag gtggtttgcg tgggcatgag tacctggcgc gtcgggtaac ggaaagcgaa
    11401 ctggtacaac gcagcccatt tatgatgctg gcggaagaag tgcctgaggc gcgggaacat
    11461 atgggaagct acgggctggc gatggtacgg cagagtgata actcatttgt attgctggcg
    11521 acacaacgaa atctactgac gctgaaccgt gcttcagccg aagaaatcca ggatcatcag
    11581 tgtgagattt tgcgttaagg cgaggagttt aggaaagtaa gtgccggata tgaaatccgg
    11641 cacctgtcag acttaagcct gtttagccgc ttctgcagct ttaacgatca cggcgaaagc
    11701 gtcagctttc agagaagcac cgccaaccag cgcgccgtcg atgtctggct gagcaaacag
    11761 ttctgcagcg ttagacgcgt ttacagagcc gccgtactga atgatcactt gttcagcgat
    11821 gttagcgtca actttagcaa tgtggtcacg gatgaatttg tgaacagcct gtgcctgagc
    11881 cggagttgca gatttgccag taccgattgc ccatacaggt tcgtaagcga taaccgcacc
    11941 ttcgaatgcc gcagcaccct gagttttcag taccgcgtcg atctgacgtg cacaaacttc
    12001 ttcagttttg cccgcttcgt tttccgcttc ggtttcaccg atgcacagaa ccggagtcag
    12061 tccctgctct ttcagcaccg cgaatttttt cgcgatcagt tcgtcagatt ctttgtggta
    12121 agtacgacgt tcagagtggc cgatgatgat gtactgtgcg ccgatgtctt tcagcatagc
    12181 agcagaggtt tcaccggtga atgcgccgga caggttcagg tccacgtttt gcgcacccag
    12241 catgatgtgg ctgccttcag cttcacgctt cgccatatcg atgtacattt ccggtggtgc
    12301 gattgcaacc gcacagccag caacacctgc cagctcttta cgcaggttag ataccagctc
    12361 gtgaaccatg tggcggctgc cgttcagttt ccagttaccc atcactaaag gatgtcgcat
    12421 tttaattctc cacgcttata agcgaataaa ggaagatggc cgccccgcag ggcagcaggt
    12481 ctgtgaaaca gtatagagat tcatcggcac aaaggctttg ctttttgtca tttattcaaa
    12541 ccttcaagcg attcagatag cgccagctta atcggttcaa cagcgaaggt cagccccttt
    12601 tcgccgttgt ccgcgacaac ataacgcagt gcaccttctg tctcggtgta ataacgtttg
    12661 tttttccccg ccgttagtag cgactgcagt tttttctggc tttgagtttt ggtcattaat
    12721 ggggtgagtg tgcggatcac cgctgccatg tatgcctgag ctttcgcttt cgcggctttt
    12781 tgctctggcc cctggatggg tagccaggtt atttgaatgc ttttgatttt taaggtaccg
    12841 cgctccagcg cagtagaagc atacaagttc tcgttaattt tacttgctgc acgagtgaga
    12901 ttggctttgt cgggactgct gtcgatggca cgaaattcgt tcagtggcag gctgggattc
    12961 tggctgttaa agtcttctcg aaactggctg atggagagat cgaaagtagg ggctccagcc
    13021 agcagataag gcgcggtcgt tgccgtatct ggtgtttgtg catgcgccgt tgttgtaacg
    13081 gttaatgcag aacataagag aaaaaacagc gtacaccctg gcttcattga cagtaccttt
    13141 tgttatgact ggctccgatt aaaacgatat ctgtcgccct tgtcaaaagg gtagaatcct
    13201 ggaaaagaca accatcaagg aacttacatg accatacagc aatggttatt ctcatttaaa
    13261 gggcgtattg gacgccgtga tttctggatt tggataggcc tgtggttcgc aggcatgctg
    13321 gtgcttttct cactggcggg taaaaattta ctcgatattc agaccgcggc gttttgcctt
    13381 gtgtgcttgc tttggccaac agcggcagta accgttaagc gcttgcatga tcgcgggcgt
    13441 tccggcgcat gggcatttct gatgattgtg gcgtggatgc tgctggcggg taactgggca
    13501 attttaccgg gtgtctggca atgggcggtg gggcgttttg tcccgacatt gattctggtg
    13561 atgatgctta tcgatcttgg tgcgtttgtt ggcacacaag gcgaaaataa atatggtaaa
    13621 gatacccagg atgtgaagta taaagccgac aataaatcaa gtaattaacc tttaatatat
    13681 ctcttatcgc tatttttttc cgggattgtt atttccacga tgcaagtgac aatgcccgga
    13741 aaatccttct gaactcttca ccttaagcaa tatcaaaaaa aatggtaatc cataagatca
    13801 ttacttgttt gttcttcctt taacggcgca ttattcttaa gtcgtaatcg acagagaggc
    13861 gaatatacag aggtgcccta tgaaagatgt cgtagataaa tgcagtacta aaggatgtgc
    13921 gattgatatc ggtacggtga ttgataacga caactgtacc agtaagtttt cgcgcttttt
    13981 tgccacccgc gaagaagcag agtcctttat gaccaaactg aaagagcttg ccgccgctgc
    14041 atcctctgca gatgaagggg ccagcgtggc ctataagatt aaggatctgg aagggcaagt
    14101 tgagcttgat gcggccttca ccttctcatg ccaggccgag atgattattt ttgagttatc
    14161 actgcgttcg ttagcttgat ggagttgctt cagaaggagt taaacatggc ttataaacac
    14221 attggcgtgg caatttccgg gaatgaagaa gatgccttac tggtgaataa agccctggag
    14281 ctcgccagac ataatgacgc tcacctgacg ttaattcata ttgatgatgg cttaagcgag
    14341 ttgtatccgg gtatctactt ccctgcaaca gaagatattc ttcaattgtt gaagaataag
    14401 tcggataaca agctgtataa actgacgaaa aatattcaat ggccgaagac aaaactgcgt
    14461 attgaacgcg gagaaatgcc ggaaacactg ctggaaatta tgcaaaaaga gcagtgcgac
    14521 ctccttgtct gtggtcatca ccactcattt atcaaccgtt tgatgccggc atatcgcggg
    14581 atgatcaata agttgagtgc ggatctcttg atcgtgccgt ttatcgacaa gtaaccgctt
    14641 accagtaatg ctccgctgtc atatggcccg gtcggcgacg taaatgtttc gtcatctgcc
    14701 gggtctcctt cagcaactgt tgtgtatcgc gcaccatctg tggattgccg cacagcatca
    14761 catggctggt ttctttattc atcggcaggc caaccgcgct ttccagttca ccactttcaa
    14821 ttaacgccgg taatcgccca gtgagcgaac ccgcagccgt ttcccgactg accaccgtct
    14881 gaatgcgcag tttcccctcg tagcgttttt ccagttcctg catcagaggt agatagctta
    14941 agtcggcggc atatcgtgcg gcgtgaacca gcaccatgtt tttgaagcgt tctaaatctt
    15001 tgcccaattg cagaatcgat aaataagggc caatcgctgt accggttgcc agcatccata
    15061 gcgtttcgca atctggtact tcatccagca caaagaagcc agcggcttcg ctaaccacct
    15121 gcacttcatc gcccggtttc agtgccgcca gtcgtggact taatttgcca tcggggacgg
    15181 tgaccaggta aaactccaga tccggattat cgggcgagtt aacataggag taggcgcgct
    15241 gaacgcgttc gccttcaatt tcaaggccaa gtttggtgaa ttgcccggcg gtaaacggaa
    15301 gcacgggagc gtgaacggtg agactaaaca gggcgtcggt ccagttctgc actttagtga
    15361 ctttgcctgt tacccaatca gccatgtttt tctcctgttt tgattgactt gccttatctt
    15421 cgttctccga acaaaaaaat tccagtcccg aaggactgga aggctcaatc gatcaaatca
    15481 atcagaggat gtgcacctgc atttccgggt ctttacgatc cagatagtgg atggactgaa
    15541 tgcgacgaat ggtgcgtgat ttaccacgga tcagcagcgt ttcggtggtg gcgatattgc
    15601 ctttgcggct aataccttcc agcagatcgc ctttggtaat accggtggca gagaagatga
    15661 cgttgtcgct gcgcgccata tcgcccaaac gcaatacttt accggcttcg atgcccatcg
    15721 ctttgcagcg tgccagctcc tgctcgccaa tgcgacgatt ctcttcgttg tcgcctttga
    15781 cgtcatgacg cgccagcaga cgaccgttca tgtcaccatc taatgcgcgg atcaccgccg
    15841 cagaaaccac gccttccggc gcgccgccaa tgccgtacag tacatcgact tcactgtccg
    15901 gcatacaggt gagaattgag gccgcaacgt cgccatccgg aatagcaaat acgcgtacgc
    15961 cgagttgttg catttctgcg ataacggcat cgtggcgcgg ttttgccaga atcgttaccg
    16021 tcagttcgct caacggtttg ccgagcgccg ccgcaacatt gcgcaggtta tccgccagcg
    16081 gcaggttcag atcaatggtg cctttggctc ccggtccgac gatcagcttc tccatatata
    16141 tatctggcgc gttgaggaaa cagcctttat cgcctactgc cagcaccgcc agcgcgttag
    16201 cctggcccat cgccgtcatg cgcgtgcctt caatcggatc aacagcaata tctaccgcgt
    16261 cgccgcgacc agtaccgact ttttcaccaa tgtagagcat cggtgcttcg tcgatttcac
    16321 cttcaccaat gacgatggtg ccgtcaatgt tgacctggtt gagcataata cgcatggcgt
    16381 ttaccgccgc gccgtccgcg gtgtttttat cgccgcgtcc taaccatttg tagccagcca
    16441 gcgctgctga ttcggtgacg cgggaaaatt cgatggcaag ttctcgtctc atagcaaact
    16501 ctaagcagta aggaatggcg cgaagtgtag cacaggggaa gggcgggatt attgatgtgt
    16561 gcggggttgc ccccgcacgg tttcagagtc tgtgtgatgc cggatgtgta tccgactcgt
    16621 aggcctgata agacgcgcaa gcgtcgcatc aggcaatcat tattactcgt cgtgttcttc
    16681 ccatgccatc gcgcgtttga ccgctttttt ccagcctgcg taacggtaat tacgctcagt
    16741 ggtttcgatg ccaggacgga actcgcgttc aatcaccgct ttctcttgca gctcgtcaag
    16801 attctgccag aagccaaccg ccagaccagc aagataggcc gcacccaaag cggtgacttc
    16861 gcgcacttcc gggcgctcaa cgcgcgtacc gagaatatcg gactggaact gcatcaggaa
    16921 attgtttgct actgcgccgc catccacgcg cagggcgtgc agtcgaatac cagagtcggc
    16981 ctgcattgct tccagcacgt cacgcgtctg ataagcaata gactccagcg tcgcgcgtat
    17041 aatgtggtta gcgttcaccc cacgagtcag accgaaaatc gccccgcgtg catacggatc
    17101 ccagtatggc gcacccagcc cggtaaaggc cggaaccaca tacacaccat tggtgttttg
    17161 cactttggtg gcgaaatatt cggaatcgta ggcgtcgtta atcaacttca tctcatcgcg
    17221 cagccactga atggacgcgc ccgccataaa cactgcacct tccagcgcat agttcacttc
    17281 gccagtcggg ccgcaggcga tggtggtcag caggccgttt tctgatttca ccgctttctc
    17341 gccagtgttc atcagcataa agcagccagt gccataggtg ttcttcgcca ttccttcttt
    17401 cacgcacaac tgaccaaaca gcgccgcctg ctggtcaccg gcgatcccgg agattggaat
    17461 acgcgtgccg cctttgccgc caatgttagt ctgaccgtat acttcggaag aacgacgcac
    17521 ttctggcagc atctcgcgtg gaatatccag cacctccagc attttgtcgt cccagtccag
    17581 agtatggatg ttaaacaaca tggtacgaga ggcgttggtg taatcggtca catggacacg
    17641 gccctgagtc attttccaga taagccacgt atcaaccgta ccaaacagca attcaccacg
    17701 acgcgcacgc tcgcgagagc cttccacatg gtcgaggatc cacttcactt tggtgccaga
    17761 gaagtacggg tcaatcacca gaccggtgtt gttacgaatg tactcttcca tgccatcgcg
    17821 tttgagatgt tcgcagatct cggcggtacg acggcactgc cagacaatgg cgttatagat
    17881 aggtttgccg gtttcttttt cccagacaat agtggtttca cgctggttcg taataccgat
    17941 agctgcaatt tgatcggaac tgatatcggc tttcgccagc acttctacca gcgtggagct
    18001 ttgggtggcc cagatttcca ttgggtcgtg ttctacccag cctggttttg ggtagatttg
    18061 ctcaaattcg cgctgcgaca cgctaatgat attggcatcg tgatccatta cgaccgcgcg
    18121 ggagctggtg gtgccctggt cgagcgcaac gatatatttt ttttcagtca tgtttaattg
    18181 tcccgtagtc atattacagc gaagcttttt gttctgaagg agttgtggtc tccttttctt
    18241 ccacaacaca gatatcgcaa ggcaaatggc gaccaatcag tttgcgatag gcaaatgcac
    18301 ctacaatcgc gccaacgata gggccaaaaa gcggcaccag gaagtaagga atgtctctgc
    18361 cgccggtaaa ggcgacattg ccccagcccg ccagccaggc aaagactttc ggaccgaagt
    18421 cacgcgctgg gttcatggca aagcccgtca atgggcccat agatgcgcca atgaccgcaa
    18481 tcagtagacc aatcagcaag ggagccaaag ggccgcgtgg tacaccgttg ccatcgtccg
    18541 ttaacgccag gatcagcccc atcagaatag cggtaatcac catctcaact gcgaaagcct
    18601 gcacaaaatt gatatgagga ttagggtaag tagagaaagt gccagccaga tcaacacttt
    18661 caacgctgcc gcgaacaatg tgatgagtct gctcgaagtc gaaaaataaa ttgtagtaaa
    18721 gcccgtaaac taaagccgca gcacagaaag cgccggcaac ttgtgaaacg ataaaaggaa
    18781 taactttgcg cttgtcgaaa caggcaaaca gccacaatgc aatggtaaca gcgggattaa
    18841 gatgcgcgcc ggaaacccct gcggtcaggt agatggccat tgccaccccc agtccccaaa
    18901 tgacactgat ttcccactga ccaaaagacg caccagcgac ttttagtgct gcaacgcaac
    18961 ccacaccgaa aaaaatcaac aacccggtac cgaggaattc agcaatgcac tggcctttca
    19021 aggttgatgt ttgactcata atcggatcct gaagagttaa tgtttgttgt atgcgtgaaa
    19081 gtcacggacc tccacgatgc ttgtaggcat gctgtaaact tatcgttaac gagcaaaaac
    19141 gagaaatatc gaacttaaaa tgtgtgtgct tcgtcataaa atgagcgtta tcgcgccatt
    19201 tattccatct gattgtacat gatgcatgtt tgttagatca ttcgcgcaac aaatttatta
    19261 acattttcga ttggtgtgga ttatgtcacc aggagaagga tgaaaagtgt ggcaaaccgt
    19321 aatctgcaag gcgtgccgct ggacagcgat ggcgcggctt catacaatcg gagctaacta
    19381 aagtgcgctc gtatttatta aggcgtcacc ggtaatcggg acgaggattt ttatccatca
    19441 acgccttgca attcaggaga ggtatgacaa tgtcattaga agtgtttgag aaactggaag
    19501 caaaagtaca gcaggcgatt gataccatca ctctgttgca gatggaaatc gaagagctga
    19561 aagaaaaaaa caactcactg tcgcaggaag ttcaaaatgc ccagcatcag cgcgaagagc
    19621 tggagcgtga gaacaaccat ctgaaagaac agcagaacgg ctggcaggaa cgtctgcagg
    19681 ccctgctggg tcgcatggaa gaggtctgat tacacttcat ctttcgcgta atttctttac
    19741 tgtgtaggcc aacaggtaac gcagaaaaaa ggcaccttgc g
//
LOCUS       DAZFRG010000075        19741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFRG010000075 DAZFRG010000000
VERSION     DAZFRG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.075"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..609
                     /gene="tssB"
                     /locus_tag="ACHKUC_002095"
     CDS             106..609
                     /gene="tssB"
                     /locus_tag="ACHKUC_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6618350.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
                     LGIAVQK"
     gene            632..2116
                     /gene="tssC"
                     /locus_tag="ACHKUC_002096"
     CDS             632..2116
                     /gene="tssC"
                     /locus_tag="ACHKUC_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6618351.1"
                     /translation="MSVKEEIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPVKTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKTFPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYIADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            2121..2546
                     /gene="tssE"
                     /locus_tag="ACHKUC_002097"
     CDS             2121..2546
                     /gene="tssE"
                     /locus_tag="ACHKUC_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6618352.1"
                     /translation="MLRRDSGPTGSLFERIREAANPPSYQNPKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELCFHIVATVDVSETRGVFEFDILLDNHQRYCVE"
     gene            2552..4393
                     /gene="tssF"
                     /locus_tag="ACHKUC_002098"
     CDS             2552..4393
                     /gene="tssF"
                     /locus_tag="ACHKUC_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6618353.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSEKSSLTAQKALPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLEAAGFDNRDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPINAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYAEPVRPDGRMAEYPL
                     RASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLLHHGLEHSIAFVHADGSIPGSSRFNDDVITTSLICTNRMIPARLHTGDI
                     CVAVNKNPAVASFRNVTRPTLPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI"
     gene            4357..5406
                     /gene="tssG"
                     /locus_tag="ACHKUC_002099"
     CDS             4357..5406
                     /gene="tssG"
                     /locus_tag="ACHKUC_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6618354.1"
                     /translation="MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQD
                     ISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLSDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            5423..6703
                     /gene="tagH"
                     /locus_tag="ACHKUC_002100"
     CDS             5423..6703
                     /gene="tagH"
                     /locus_tag="ACHKUC_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001511117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO6618355.1"
                     /translation="MAEEKQQSRQASLTLQVMNGNELESGRAAKCLFSENGGDIGHTP
                     ECHWQVQDRAGSIAARACTVIRHDGAYCLRCLTPGLMINLAPASSDALIRLRQGDEIQ
                     LGALALKVFLHDGAAVTYDERMATPETIVMNRDSLADTLLTTEGQPAYPGMPFQHQLA
                     PTVAHGFSSDPLQALQTESLMVADDPIAPRVSRPVAPASDLTGANGINTPFMDLPPGN
                     ARHEDGDEFSAMAQYHLAVTPLLRGMECPLTLHNSQDADEFLEEAGRALQAAIKGLLS
                     LQQQQNSLSDKHLRPLEDNPLRLDMDYATALNVMFAEGKSPVHLAAPAAIAESLRNIR
                     HHEEANRAAIVEALRVMLDAFSPGNLMRRFAQYRRSHELRQKMDDAWAWQMYSNYYDE
                     LASSRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            6700..7224
                     /gene="tssJ"
                     /locus_tag="ACHKUC_002101"
     CDS             6700..7224
                     /gene="tssJ"
                     /locus_tag="ACHKUC_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6618356.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFHAADYDQVATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIESVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            7227..8573
                     /gene="tssK"
                     /locus_tag="ACHKUC_002102"
     CDS             7227..8573
                     /gene="tssK"
                     /locus_tag="ACHKUC_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6618357.1"
                     /translation="MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLTRRLDALGDFFW
                     GFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIY
                     LALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
                     ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVRVSGLLGAFSGEVQGLLATRA
                     ADLAGRIGSPEQSGIADVAEFMMLQMLNRYQMQFTHRSQLHTLHPEAFYRDLVGLLGE
                     LMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTW
                     IATINDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLH
                     TLTAAPRQLPYHEGYVYFELEKGTPAWQDVIKAGALALHISGSFPDLNLQLWAIRG"
     gene            8578..9342
                     /gene="icmH"
                     /locus_tag="ACHKUC_002103"
     CDS             8578..9342
                     /gene="icmH"
                     /locus_tag="ACHKUC_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO6618358.1"
                     /translation="MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLG
                     MVMRLSTMNSQAMPEHLFTQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAAL
                     GNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
                     RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKH
                     VLCGGLVVLTVFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9352..12114
                     /gene="tssH"
                     /locus_tag="ACHKUC_002104"
                     /gene_synonym="clpV"
     CDS             9352..12114
                     /gene="tssH"
                     /locus_tag="ACHKUC_002104"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6618359.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHVLLQ
                     MLAIPRNDVRVIAERTGISAEDLRQALTVESYPGGRSAEGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTAINRDQLQQDFAAWTKESAESV
                     DLAGGQTPRATETGDTLLARYAKNMTADARNGRLDPVLCRNYEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVIFSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLEREL
                     RIGLRTDTSRMTEVLEQYDETLSALDELEVAWQQQQTLVQEIIALRQQLLGVAEDDVA
                     SLPDADAVEDTPPEAEQDSTDAESADDAGSVQPEETAETVSPVQRLAQLTAELDALHN
                     DQLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGGTIKGQDLAIASL
                     HKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQ
                     EKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDK
                     GEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADFFKPALLAR
                     MEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGADVIIGPEVTDEIMSRVTRAENGA
                     RMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDALPDDAVTPQT
                     EDETVL"
     gene            12111..12854
                     /gene="vasI"
                     /locus_tag="ACHKUC_002105"
     CDS             12111..12854
                     /gene="vasI"
                     /locus_tag="ACHKUC_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO6618360.1"
                     /translation="MSRLCVFRQTGWFIAGLMAGLPASAAPAETSSVAGVAVAVATAT
                     PPDATATLQAMQSCRQETAALERLDCYDRILAPEQAGFGGAALVKARYQGEAWARATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGTD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            12859..14301
                     /gene="tssA"
                     /locus_tag="ACHKUC_002106"
     CDS             12859..14301
                     /gene="tssA"
                     /locus_tag="ACHKUC_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO6618361.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFVDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATIQRDALLGELAKLAQCWQAHN
                     ASELAKATDDLFSLFQRAFRDVAPEMTSSARSAAIAPQATTGYVPDMSPHTVAAQPPV
                     AATPIPQVTVESHDDKAWRDTLLKVAAILCERQPESPQGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YKQVADAIRDEVTDFLARLPALINLLFNDRTPFVSEQTKQWLASSGSVNQTVPVVQTD
                     EELQAAKACFDENGLEAALRYLENLPEGDPRHQFHRQFFGAQLLEEAGMVQLAQQQYR
                     MLFRTGLHMMLSEWEPSLLKALEQKLTAEQ"
     gene            14381..17848
                     /gene="tssM"
                     /locus_tag="ACHKUC_002107"
     CDS             14381..17848
                     /gene="tssM"
                     /locus_tag="ACHKUC_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO6618362.1"
                     /translation="MPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANR
                     WLATAVWGLIALVWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLR
                     LRRHLDNRRYLWQLPWYMVIGPAGSGKSTLLREGFPSDIVYTPESIRGVEYHPLITPR
                     VGNQAVIFDVDGVLTTPGGDDLLRRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLT
                     ADKSRRETLVQNLRQQLQEIRQSLHCRLPVYVVLTRLDLLNGFAALFHSLDKKDRDAI
                     LGVTFTRRAHESDGWRSELGAFWQTWVQQVNLALSDLVLAQTGAAPRSAVFSFSRQMQ
                     GTGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLAT
                     WPLVETTPYFTRRLFPEVLLAEPNLAGENSVWLNSSRRRLTAFSTCGAALAALMVGSW
                     HHYYNQNWQSGVNVLAQAKAFMDVPPPQGTDEFGNLQLPLLNPVRDATLAYGDYRDHG
                     FLADMGLYQGARVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNIAPPESEEKLAVLRVV
                     RMMEDKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMAHLDYALEHTDWHAQRQSGDS
                     DAVSRWTPYDKPIINAQQELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNV
                     FVAGNDEKLVIPQFLTRYGLQSYFVKQREGLVELTALDSWVLNLTQSVAYSEADREEI
                     QRHITEQYLSDYTATWRAGMDNLNVRDYETMPALTDALEQIISGDQPFLRALTALRDN
                     THALTLSGKLDDKAKEAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQL
                     TELHRYLLAIQNSPVSGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLA
                     DQAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKPDG
                     ILDNFYKNNLRLFLENDLTFGDDGRMLIREDIRQQLDTAQKIRDIFFSQQNGLGAQFA
                     VETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGRAP
                     RSIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYQVHVDTEDNPFTGGLFS
                     LFRLPDTLY"
     gene            17859..19217
                     /locus_tag="ACHKUC_002108"
     CDS             17859..19217
                     /locus_tag="ACHKUC_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO6618363.1"
                     /translation="MSNYALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKL
                     VESLALSIFKANGVDLHTATYYTLARTRNQGLAGFCEGAELLAAMIIHEWDKFWPQSG
                     PARTEMLDWFNTRTGNILRQQVSFSENDLSLLYRTERALQLICDKLQQVELKRQPRVE
                     NLLYFVQNTRKRFEPQPRNRTDTAAQTMVRTLVYAPEGTASATAETMPPLPDLPEMRV
                     GVRGVADNADKAKQGDTVKGFVAGAACTAVIASALWWWQAYPMQQQLTQVRDTTQGAT
                     TVWLASPVLKEYEQYLQQLLNAPPLQPLETGMQMMRTADTLWPESLQQQEASRMWSNT
                     LRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQET
                     PLEYLLTQYQETRTQKQDTQALEKEINERLDGLLSRWLLLKNTGQDMATDNRTEPVHP
                     TH"
     gene            19235..19714
                     /locus_tag="ACHKUC_002109"
     CDS             19235..19714
                     /locus_tag="ACHKUC_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6618364.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLANARLSNIGLTIPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         4575 a         5563 c         5505 g         4098 t
ORIGIN      
        1 caacagaagg aaggttacat tatctaactg ccactgaaac ggtattgtcg gtatttgtat
       61 gcagtacgaa aatgctgtgc tcatggcctg aacgggaaca tttttatgag caaaatgaac
      121 aacaatggcg gcagtgtcgc gccgaaagaa agaatcagcg ttcggtatac accaaaagtt
      181 gatggggtgg ctgctgatat cgaattgccg ctgaatttat tgatcaccgg caatctgaaa
      241 gggaaaccag ataacacgcc gttggatgag cgaactgcca ttgctatcaa ccgctataac
      301 ctcaacgcag tcatttcaga agctgacatt gaacgggagt ttgttgttcc ggctgaactc
      361 agtgacgctc ccaatgagca aatgtacgtc aacctgaaag tcaaatctat ggatgacctt
      421 tctcctgacc atattgccag ccaggtacca gaaataaaac gtctgctcga attgcgtgaa
      481 gccctggtcg cacttaagtc tccactaggc aatattccag ccttccgcgc acaacttcag
      541 gcgctactgg aaaacgaaga cacccgcgag cagctgatcc aggaactggg tatcgccgtt
      601 cagaaataat tttcacagaa ggatattcag tatgtcagta aaggaagaaa ttgctcccgt
      661 cagcgcgtct caaaccgccg cgcccccatc tctgcttgat gaaatcatgg cgcaaacccg
      721 cgtccagcct aaatcagaaa gctacgatat cacccgccag ggcgtgagcg cctttattgc
      781 cgccatgtta cagggcgaca gtagcgctga acctatcaat atcctggccg ttgacgccat
      841 gattgctgat attgatgccc gaaccagcag gcagatggat gccattattc atgccccgga
      901 gtttcaggaa ctggaatccc tgctgcgctc gctgaagctg ctggttgaac gcgcggatac
      961 acgggaaaac atcaaagtcc atttcctgaa tgtcactcag gaagaactgc tggatgattt
     1021 cgaatttgca cctgaaatca cgcagtctgc gtattacaag catgtatatt caagcggtta
     1081 cggacaattt ggcggtgagc cagtggcggc ggtaatcggc aactttgcct ttaagaacac
     1141 cacgccggat atgaagctgc tgaaatatat cagccaggtc agcgcgatgg cgcacagccc
     1201 gttcctgtcg tcagtatcat ccgaattttt cggtctggat tcatggacag agttaccggg
     1261 gatcaaagaa ccgggtgcca tctttgaagg cccggcctac tcccgctggc gcgccctgcg
     1321 tgaatcggaa gattcccgtt acctgggcct gaccgccccc cggttcctgc tgcgccaccc
     1381 ttactcaccg gatgaaaacc ctgtcaaaac cttccgttac catgaagatg tcagccagag
     1441 tcatgagtct tatctgtggg ggaatacctc gtttttactg gccgccaacc tggcggaaag
     1501 ttttgctaag tatcgctggt gcccaaatat tattggccct tccagcggcg gcgcagtcaa
     1561 agacctcccg gtgcatctgt atgaatccat ggggcaaatg caggcaaaaa ttccgactga
     1621 agtgttgatc actgaccgga gagagtatga gctggcggaa gagggcttta tcacactgac
     1681 catgcgtaaa gggtctgata atgcgtgctt tttctcggcc aactcggtgc agaaacccaa
     1741 aacattcccc aaaacccccg aaggcaaggc tgcggagacc aactataaac tcggcaccca
     1801 gctgccatac ttgtttgtta tcagcaggtt agcgcattac atcaaggtaa ttcagcgcga
     1861 acagcttggc tcatggaaag aacgtagtga cctggagcgt gagctgaaca cctggattcg
     1921 ccagtatatt gctgaccagg aaaatccgcc tgccgaagtc cgcagccacc ggcctttgcg
     1981 tcaggctaag atcgaggtac tggatgtgga tggtgagccg ggctggtacc aggtcgcgat
     2041 ttctgtcaga ccgcatttta aatacatggg tgccagtttc gacctgagcc tggtcggacg
     2101 actggataag gaataatccg atgctaagac gggattcagg accaaccggc agtctttttg
     2161 aacgtatccg tgaggcggca aatccgcctt catatcagaa tccaaaagaa gcactgatac
     2221 gttccatcag acgtaacctg cgacaggtcc tgaatacccg ctccggcagt tgttatggtt
     2281 caccggagct ggggattact gatttaaatg atgaatcgct ggcctccagc gatttcagac
     2341 gagaaatccg taagtccatt agccagtgca ttttacatta cgaacctcgt attaccgatg
     2401 ttgttgttac ggctgctgca ccggatgaat acgcccctgt ggaactgtgt tttcatattg
     2461 tggctacggt cgatgtcagc gaaaccaggg gagtgtttga atttgacatt ttgctggaca
     2521 accatcaacg ctattgtgtg gagtgatctc tgtggctttt gaagaacgtt actaccgcga
     2581 agagctggat tatcttcgcc agctaggcaa actgctggcg caggaaaaac cacatctggc
     2641 ccactttctg gccgagaaag agggtgatcc ggacgtggag cgtctgatgg aagcctttgc
     2701 ctttatgtcc ggtagcctgc gccagaaact tgaggacgaa ttccccgagt tcacccatgg
     2761 gctgatccgt atgctgtggg caaactattt acgcccggtt ccggccatga cggttattgc
     2821 ctatgaaccc aaaaccgatc agttaaaagt ccctgttcag gtctgtcgta atgaactgat
     2881 cagaagccgc tcagaaaagt cctccctgac tgctcagaag gcgctgcctg acaatcatga
     2941 taaaatcgtt tcttccgtag cctgccattt cacgctggcg cgcgatatct ggctgcaacc
     3001 actgcgcatt ctcgataccc gcaacgccag cagcctgaaa gaaggcatca tagatatcag
     3061 ttttaccgca gataacaatg tctcccccgc catgcttgac ctgaacaaaa tcacgttctg
     3121 gctgggtaat gaagacgatt atacccgaca ccagctttat ctgtggtttt gcgaatgcct
     3181 gatggatgcg gaactgattg ccggagagta ccgcctgccg ctgccggatc tgtggctgga
     3241 ggcggcgggt tttgacaacc gggatgctct gctgccgtgg ccgaaaaatg tccacagcgg
     3301 ctaccgcgtg cttcaggagt atttctgcta tcccgaagcc ttcttctttt tccatctgcg
     3361 cgatgttgcg ccgctaccag acgatttccc gatcaatgcg tttacgctgc gcctgcactt
     3421 taaccgcccg ttacctgccg acatcaagct gcgccaggat tcgctgcgtc tgcactgtac
     3481 gccagccatt aacctgttta cgcattatgc ggaacccgtc aggccggatg ggcggatggc
     3541 ggaatacccg ctgcgcgcca gccataagca tccggacgcc tatgacattt ttcaggtcag
     3601 cacagtcacc agtaaagtca aagtttcagg tactgaaatc agcccaggta gccaggcgcg
     3661 tgtctggccc gaatttgaaa gcttccagca ccagatggag tacagccgcc agcgagaagt
     3721 cgtctactgg catcaccgga ctaaaacgtc cctgcttcac cacgggcttg aacactctat
     3781 cgcctttgtc catgcagacg gtagcattcc gggcagctca cggtttaacg atgacgtgat
     3841 caccacgtca ttaatctgta ccaaccgtat gatccctgcc cggctgcata ccggtgatat
     3901 ctgcgtggcc gtcaacaaaa atccggcagt ggcctcattt cgtaatgtca cgcgcccgac
     3961 actgccgctc tggccagtca cggacggcga catgcactgg tcgctgattt ccgcgatgaa
     4021 cctgaactac ctctccctgc tggacaggga gacactgatc cagatcctgc ggaccttcga
     4081 cctgccaggt gctcaccatc cgcagcgggc cagactgtca cgccagaaac tggatgccat
     4141 agaaaaactc gaaaccaaac ctgttgacag gttgtttaaa ggcgtaccgg ttcgcgggct
     4201 ggcgaccacg ctgtggatac gtcccgatcc gtttatctgc gaaggtgaaa tttatcttct
     4261 gggtaccgtg ctctcgcact tctttgccct ctacgccagc atcaattcgt accactgtct
     4321 gaaaatcatt aacacggaaa gtcaggagtc ctgggaatgg caggagaaaa tgggccagca
     4381 cgccctgata tagcgacaaa accgccgtta ccggcggatg tgcgcaactg caatttctat
     4441 gtgctgatgg aggcgctgta ccgtcggcat ggcgcgccgg gccaggacat ttccctgcgt
     4501 accgagcctg cccgggagat cgtgcgtttc agctctgacg ccagtatcag ttttccgggt
     4561 acggacctga gtgcgctttc ccgtagccag aacggacagt atgtactgca aacccggttc
     4621 ctgggttttt ccggcagcca gtcgccgcta ccgggctatt atctggatca gatggcgcag
     4681 gagtccgcac agaacgaaga cggcctgaaa gaatttctcg atctattcag ccaccgctgg
     4741 acacagtttg cctatcatgc ctggcgcaaa taccgctact acatctgctt tcgtagcggc
     4801 ggtactgaca ccttttcaca gcgcatgtac gcactggtcg ggctgggtaa tcagagcgtg
     4861 cgtgacaggc ttgccattaa ccacagcaaa atgctggcct acgccggaat tctggcaaca
     4921 ccaggccgcg cgccggaggt tatctgtaat ctggtgtcgc actgttttga tttgccggat
     4981 gtcaccattg aacgctggca actgcgtaaa gtcgccattg atccggcaca ccagaaccgg
     5041 ctgggtgtac gtaatccgaa aggaaaaacg gctggacata ttccggggcg ctccatactc
     5101 ggcgttaatt ttaccctcgg cgcacgggtg ccggatcgca gcggaaaatt cctgttgcag
     5161 attggcggcc tgtcgcggga gcgttatctc tcatttttac ccgatggcga aaatcacctg
     5221 ccgctgacca tgttcctttc ctttattctg cgcgaccagt ttgcctggga tttacggctg
     5281 tgcctggcgc cacagcaggc taaagggatg cgccttagcg atccggccag ctcccgcctc
     5341 ggcagaacca gctttattgg tcagccgaaa gtaccgcccg ccatcacaat ccgaatcagg
     5401 gaataattct atggaaccac ttatggctga ggaaaaacag caatcccgcc aggcctcact
     5461 gactttacag gtgatgaatg gcaacgaact ggaaagcgga cgcgccgcaa aatgcctgtt
     5521 cagcgaaaac ggcggtgata ttggccatac gccggaatgt cactggcagg tacaggaccg
     5581 ggcgggaagc attgctgcgc gtgcctgtac cgttatccgg cacgacggag cctattgcct
     5641 gcggtgcctg acgccgggac tgatgattaa tctggccccg gcctcttccg atgcgctcat
     5701 ccgcctgcgc cagggggatg agatccagct cggcgcactg gcgctgaaag tgtttctgca
     5761 tgacggtgca gctgttacct atgacgagcg gatggcgacc ccggaaacga ttgtaatgaa
     5821 ccgcgacagt ctggcggaca ccctgctgac caccgaggga caaccggcgt atccgggaat
     5881 gccgttccag catcagcttg cccccacggt ggcgcacggt ttttccagcg atccgctcca
     5941 ggcactccag acggaaagcc tgatggtggc agatgatccg atcgccccac gcgtttcccg
     6001 gcccgtagcc cccgcatccg atctgacagg tgcaaacggg atcaacacgc cgtttatgga
     6061 tctgccgccc ggcaatgccc gtcatgagga cggcgatgaa ttttctgcca tggcgcagta
     6121 ccacctggcc gtaaccccgc tgctgcgcgg tatggagtgt ccgctgacgc tgcacaattc
     6181 tcaggatgcg gatgagtttc tcgaagaggc cggacgggcg ttgcaggccg ccattaaggg
     6241 attgctgagt ctgcaacaac aacagaacag cctgtcggat aaacacctgc gtccactaga
     6301 agacaacccg ttgcgccttg atatggacta tgccaccgcg ctgaacgtca tgttcgccga
     6361 gggtaaaagc ccggtacatc tcgcagcacc tgcggccatc gccgaaagcc tgcgcaacat
     6421 ccggcatcat gaagaggcta accgggcggc gattgtggaa gcgttgcgtg tcatgctgga
     6481 cgccttttcg ccgggcaacc tgatgcgccg ctttgcgcaa taccgccgca gccatgaact
     6541 gcgccagaaa atggacgacg cctgggcatg gcagatgtac agcaactatt acgatgagct
     6601 ggcctccagc cgacagcagg gatttgaaat gctgttcaat gaagtttatg cccaggtcta
     6661 tgaccgggtg ctgcgcgaaa aacagcggga gccggaagca tgaacggcaa agcgtttctg
     6721 gcctgcgttc tgatgagcgt cgtattaact ggctgtgaaa cagcgaaaaa aatcagccag
     6781 gtgatccgca atccggatat tcaggtcgga aagctgatgg atcagtcaac cgagctgacc
     6841 gtcacgctgc tgaccgagcc ggacagcaac ctgacggcgg atggcgaagc cgcgccggtg
     6901 gatgtccagt tggtttatct gagcgacgac tcaaaattcc atgccgccga ctacgaccag
     6961 gttgccacca ccgcgctgcc cgacgtgctg gggaaaaact atatcgatca ccaggacttc
     7021 aacctgttgc cggataccgt aaaaacactg ccgccgatca agttggatga gaaaaccggt
     7081 tatatcggtg tcattgccta tttttcagac gaccaggcca cagaatggaa acaaattgag
     7141 tcggtagaaa gtatcggcca ccactatcgc ctgctggtgc atatccgcgc cagtgcgatt
     7201 gagatgaaaa aagaggaaaa ctgacgatgg caacaatgaa aaacaaagtg atctggcagg
     7261 aagggctgtt tgccctgccg cagcattttc agcaacagca gcgtcactgc gagtacctgc
     7321 tgacccgtcg tctggatgca ctgggtgatt ttttctgggg ttttacggaa ctctccatca
     7381 atacagaact gcttgcccag ggcaaaatca tgattgaccg tgcggcgggc tgtatgcctg
     7441 acggcaccgt attcagcatt cccgaccagg atctgctgcc ggaacctttc cagccgggaa
     7501 ccctttcatc gaaagagagc cacaatattt atctggcgct gccggtgata agcgacgtga
     7561 tcaatgaaat ccaggggttg cacagcgccg gtcagggtac ggagcgttac cgcctcaccc
     7621 atacccgcgt ccgtgatttt cacactgacg aaggcgacga acagccagtc ggtctggggc
     7681 agttgatccc caggatagtc agcggcgcag atgatctcag cgcgatggtg actctccccc
     7741 tgtgccgtat cctcaataaa aatgccaccg gcgcgctggt gctggacaac acctttattc
     7801 ccaccattca ggccgtccgt gtcagcgggt tgctcggggc attctccggc gaggtgcagg
     7861 gactgcttgc cacccgcgcc gctgacctgg ccgggcgcat tggttcgcct gaacagagcg
     7921 ggatcgccga tgtggccgaa tttatgatgt tgcagatgct taaccgctat caaatgcagt
     7981 tcacgcatcg ttcccaactg cacacgctgc atccggaagc cttctaccgg gatctggtgg
     8041 ggctgctggg agaactgatg acctttaccg aaggtaatcg cctgccctgc accgtggagc
     8101 cgtataatca ccgggatctt accgcgacct tcaccagaac cgtgatcccg gaactgcggc
     8161 gcgcgctcaa taccgtactg gtgccgcgtg cgcagaatct gccgttgttc ttttcagagg
     8221 ggacctggat cgccaccatc aacgatccca cattgttgca gtcctgcaag ctggtactgg
     8281 cagtgcgcgc caggatgccc tacgaccagg tacaacgcca gttcatccag cagtcgaaag
     8341 tggccgctac cgacaaaatc cgcagcgtgg tcagcgtgca ggtgccgggt attccgctgc
     8401 acacgcttac cgctgcgcca cgtcagttgc cataccacga aggttatgtt tacttcgaac
     8461 tggaaaaagg caccccggcc tggcaggacg tgataaaagc gggcgcactg gcgctgcata
     8521 tctccggcag cttccccgat ctgaacctcc agctctgggc tataagggga taagtgaatg
     8581 agcgatatga gtgaagcgtc ggtttttccc gtggagccgg agagcggaac accaggacgc
     8641 cagtaccgtc tcgcccttcg cggcaacagc ctgaatccaa tgattgacgc cgccacgccg
     8701 ttgctcggca tggtgatgcg tctgtccact atgaacagtc aggccatgcc ggaacatctg
     8761 tttacccagg tagtaaccga tgtccaggcc gtagagcaac tactccagga acagggatac
     8821 gaaccgggcg tcattgtttc gttccgttac attctctgca cgtttattga tgaagcggcg
     8881 ctgggcaacg gctggtccaa caaaaacgaa tggatcaaac agtcgctgct ggttcatttt
     8941 cataacgaag cctggggcgg cgagaaagtg tttatcctgc tcgaacgcct gatccgggag
     9001 ccggtacgtt accaggatct gctggaattt ttatacctct gtttttcgct cggctttcgc
     9061 gggcgctaca aggttgccat tcagcaacag gatgaatttg agcggatcta ccagcgcctg
     9121 caccatgccc tgcacaaact gcgcggtgac gcgccatttc cgctacttca tcagaagaac
     9181 aacatacagg gtgggcgtta tcagttaatc cgacgactga ccatcaagca tgtactgtgc
     9241 ggcggtcttg ttgtgctgac cgtattttat ctgttttatc tgctgcgtct ggacagccag
     9301 acacaggata ttctgcatca gctcaataaa ttactccgat aaggacatct tatgatccag
     9361 attgatttag ccacgctcgt aaagcggctt aacccctttg caaaacaggc gctggaaatg
     9421 gctgcctcgg agtgtatgag ccagcaggcc agtgagatca ccgttgccca tgtgctgtta
     9481 cagatgctgg caatcccgcg caacgacgta agggtgattg ccgaacgcac aggcatcagt
     9541 gcggaggatt tacgccaggc gctgaccgtg gagagttatc ccggtggacg ttccgccgaa
     9601 ggctatccca gcttctcccc gatgctgatc gaatggctca aggagtcatg gctgctggcc
     9661 tcagcccaga tgcagcacag cgaactgcgc agcggcgtcc tgctgcttac tctgctgcat
     9721 tcaccgctgc gctatattcc acccgctgct gcccgtctgc tgacggccat aaaccgcgac
     9781 cagttacagc aggatttcgc agcatggaca aaagaatccg ccgaatccgt ggatctggct
     9841 ggcgggcaaa cgccccgcgc aacggaaacc ggcgacaccc tgcttgcccg ctacgccaaa
     9901 aacatgaccg cagacgcccg taacggcagg cttgacccgg tactgtgccg caactatgaa
     9961 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaatccggt ggtagtgggc
    10021 gaagcaggcg tgggtaaaag cgcgctgatt gaagggctgg cgctgcgcat cgtggcaggc
    10081 caggtgccgg acaagctgaa aaacaccgat atcatgaccc ttgacctggg cgcattgcag
    10141 gccggtgcgt cggtgaaagg tgagtttgaa aaacgcttca aggggctgat ggcggaggtc
    10201 attttctccc cggtgccggt cattctgttt atcgacgaag cacataccct gattggcgcg
    10261 ggcaaccagc agggcgggct ggatatatcc aacctgctca aaccagcgct ggcgcgcggc
    10321 gagctgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatattt cgagaaagat
    10381 gcagctttgt cgcgccgctt ccagttagtg aaggtcagcg aacccaacgc tgccgaagcc
    10441 accattattc tgcgcggttt gtcggcggtc tatgaacaat ctcacggcgt gctgattgat
    10501 gatgacgcct tgcaggccgc tgcgacactg agcgagcgtt atctctccgg gcgtcagttg
    10561 ccggacaaag ccattgatgt gctggacacc gcctgcgccc gtgtggccat caacctgtca
    10621 tcgccgccaa aacaaatctc ggcactgacc accctgagcc accagcagga ggcagaaatt
    10681 cgccagcttg agcgcgagct tcgcatcggg ctgcgtaccg acacctcacg aatgactgag
    10741 gtgttggagc agtatgatga aacgctgtcg gcgctggacg aactggaagt cgcctggcag
    10801 cagcagcaga cgctggtaca ggaaattatc gcgctgcgcc agcagttact gggcgtggca
    10861 gaagacgacg ttgcgtcgtt gccggacgca gatgccgtgg aggatacgcc gccagaggca
    10921 gaacaggata gtaccgatgc cgaatcggct gatgatgcag gcagcgtaca gccggaagag
    10981 accgctgaaa cagtttcccc ggtacaacgg ctggcacaac tcactgccga actggacgcc
    11041 ctgcataacg accagttgct ggtttccccg catgtcgata aaaaacagat tgcagcggtg
    11101 attgctgaat ggaccggcgt gccgcttaac cgcctgtcac agaatgagat gtcggtcatc
    11161 accgacctgc cggtatggct cggcggcacc atcaagggcc aggacctggc gattgccagc
    11221 ctgcataaac acctgctgac cgcacgcgcc gacctgcgcc gtccgggacg cccgcttggt
    11281 gcgttcctgc tggctggccc cagcggcgtg ggtaaaaccg aaaccgtcct gcaactggcg
    11341 gaactgctct atggcggacg tcaatacctg accaccatca atatgtctga gttccaggag
    11401 aaacacaccg tttcgcggct gattggctct cctccgggtt atgtcggcta cggtgaaggc
    11461 ggcgtactga ccgaagcgat tcgccagaaa ccgtactcgg tggtgctgct cgatgaagta
    11521 gaaaaagcgc acccggatgt actcaacctg ttctaccagg cgtttgacaa gggcgagatg
    11581 gcagacggtg aaggccgcct gattgactgt aaaaatatcg ttttcttcct gacctccaac
    11641 cttggctacc aggtgattgt cgaacacgcc gatgacccgg aaaccatgca ggaagccctc
    11701 tatccggtgc tggcggactt ctttaaacct gctctgctgg cgcgcatgga agtggtgcct
    11761 tacctaccgc tgtcgaaaga gacgctcgct accattattg ccgggaaact ggcccgcctg
    11821 gataacgtgc tgcgcagccg ctttggcgcg gacgtgatta ttgggccgga agtgacggac
    11881 gaaatcatga gccgcgtcac ccgcgcggaa aacggcgcga ggatgctgga atcggttatc
    11941 gacggcgaca tgctgccgcc gctctcgctg ctgctgttgc agaaaatggc ggccaacacg
    12001 gcgattgccc ggattcgcct gtcggcggca gacggtgcgt tcacggcaga tgtggaagat
    12061 gcgctgcctg atgatgccgt gacaccgcaa acagaggatg aaacggtttt atgagcaggt
    12121 tgtgcgtttt ccggcagaca ggatggttta tcgcgggcct gatggcgggc ttacccgcca
    12181 gtgccgcgcc cgcagaaaca tcgtcagtgg ccggtgtggc ggtagctgtc gccacagcga
    12241 caccgccgga tgcgacagcc acgttacagg ccatgcagtc ctgccgccag gagacggcgg
    12301 cgctggagcg gcttgactgt tatgaccgca ttcttgcgcc ggaacaggct ggctttggtg
    12361 gtgccgcact ggtgaaagct cgttaccagg gtgaagcctg ggcccgcgcg acagaacagg
    12421 aaaaacgccg ccagggcaac accacggagc tgctggtaac gcaagtaccg ggtgaacgcc
    12481 caacggtggt gataaccacc ccggctatcg gccatgttcc gccacgcccg gtgctgatgt
    12541 tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct ctggatgtcc
    12601 atgatatcgc cgtcaccctg aatgccgaca gccgggcatt gcgcagccac tggtttgtgc
    12661 gcgagaacgg caccctgctg gagtccagcc gggggttatc cggtatcgat gaaattaagc
    12721 aactgttcgg cgcaaaaacg ctgacggtgg ataccggcac agacaacgcc gccggaaagc
    12781 tgacctttaa cattgacggg ctggcacggg ttattgcgcc gctacgcgac gcctgccact
    12841 gggcgggaga ataagaatat ggcaatggac ttacgtaatc ccgatgtatg gcttgcacac
    12901 ctgctggaaa acctgccaga agataagctg tccgccgcgc ttgatgatgg caatgcagac
    12961 tgggagtttg tcgacagcga aatcgtcaag ctcggttcac tggcgcacag ccagcttgat
    13021 atcccggaac ttcagcgccg gggcctgatg cttctggcct cagaaaccaa agattttcgc
    13081 ctgctggccc acttgctgcg taccctgcaa catgccggtg atattctgct ggcctccagg
    13141 atgctggcac aatatacaga gcactactgg acatgcgccg caccgcagaa catggcccac
    13201 aaaaaacgct ttgccgccca ggtgataaaa cgctttgagt ctgccgttca ggactttgcc
    13261 gggaatgctg cgacaataca gcgcgatgct ttgctgggag agctggcaaa actggcacag
    13321 tgctggcagg cgcacaacgc gtctgagctg gcaaaggcga cagacgattt attttccctg
    13381 tttcagcgcg cgtttcgtga tgtagcgcct gaaatgacat catctgcacg aagcgccgcc
    13441 attgcgccgc aggccacgac cggatatgta ccggatatgt caccgcacac ggttgccgct
    13501 cagcctcctg ttgcagccac tccaatcccg caggtcaccg ttgaaagcca cgacgataaa
    13561 gcatggcgcg acaccctgct aaaggtggcc gccattctct gtgagcgcca gccggagtca
    13621 ccgcagggtt accgactgcg ccgccatgcg ctgtggcaaa ccatcaccag taccccgcag
    13681 gcagaaagcg atggccggac cccactggca gccttccctg tcgatatgat ggatgattac
    13741 ctggcgcggc tgaacaatgc cgacatggcg ctgtggcagc aggtggaaaa aagcctgctg
    13801 ctggccccgt actggctgga cggtcattac ctctcagcac agaccgcgct gcgcctcggt
    13861 tataaacagg tggcggatgc tatccgtgat gaagttaccg attttctggc ccgcctgccc
    13921 gcactgatca acctgttgtt taacgaccgc actccgtttg tgtccgaaca gacgaaacag
    13981 tggctggcat catccggctc agtcaaccag accgtcccgg ttgtacagac cgatgaagaa
    14041 ttacaggcgg ccaaggcgtg cttcgatgaa aacgggctgg aagccgcgct gcggtacctg
    14101 gaaaacctgc ccgaaggtga tccccgccac cagtttcacc gccagttttt cggcgcgcag
    14161 ttactggaag aggccggtat ggttcagctt gcacaacagc agtaccggat gctcttcaga
    14221 acaggattac acatgatgct atccgaatgg gagccttccc tgctgaaagc actggaacaa
    14281 aaactcacgg cagaacagta aaagataaca aggagttact gtgttcaaat ttcccacatc
    14341 ccgactgttc agcacgttga aatctgcgct caggccagcg atgccgcggt ttaaagtttc
    14401 cgccacctgg ctactgacgc tggcatggat ttttctgctg gtgtggatct ggtggcaggg
    14461 tccaaaatgg acgctctatg agcagcactg gctggctccg ctggcaaacc gctggctggc
    14521 gaccgccgtc tggggactta tcgctctggt ctggctcacc tggcgggtga tgaagcgtct
    14581 gcaaaagctg gaaaaacagc agaaacagca gcgggaggaa gaaaaagatc cgttgaccgt
    14641 ggaactccac cgccagcagc aatatctgga tcactggctg ctgcgcctgc gccgccatct
    14701 ggataaccgc cgttatctgt ggcagttgcc gtggtatatg gtcattggtc ctgcgggtag
    14761 cggcaaaagc acgctgctgc gcgagggctt tccgtctgac attgtttaca cgccggaaag
    14821 catccggggt gtggaatacc acccgctgat cacaccgcga gtgggcaacc aggcggtaat
    14881 tttcgatgtt gacggcgtac tgaccactcc cggcggggat gatctgctcc gccgccgcct
    14941 gcgcgaacac tggctgggct ggctgatgca aacgcgcgct cgccagccgc tcaacggtct
    15001 tatcctgacg ctcgatcttc ccgatctgct gacggcggat aaatcccgcc gtgagacact
    15061 ggtacaaaat ttgcgccagc aacttcagga gatccgtcag agcctgcact gccgtctgcc
    15121 cgtttacgtg gtgctgacac ggctggatct gctgaacggc tttgccgcgc tgttccattc
    15181 actggataaa aaagaccgcg atgcgatcct cggcgtcaca tttacccgcc gcgcccatga
    15241 aagtgacggc tggcgcagcg aactgggggc tttctggcag acgtgggtac aacaggtgaa
    15301 cctggcgctg tcggatctgg tgctcgcaca aaccggtgct gctccccgca gcgctgtgtt
    15361 cagcttctcc cgtcagatgc agggaacagg agaaatcgtc accgcactgc tcgccgcatt
    15421 gctggacggt gagaacatgg atgtaatgct gcgtggcgtc tggctcacat cctcgctaca
    15481 gcgtggccag gtggatgata ttttcacgca gtccgccgcc cgccagtacg gactgggtaa
    15541 cagctcgctg gcaacctggc ctctggtgga gacgacgccg tattttactc gccgcctctt
    15601 cccggaagtc ctgctggctg agccgaacct ggcgggtgaa aacagcgtct ggctgaacag
    15661 ctcccggcgc aggctgaccg ccttttccac ctgtggcgcg gcactggcgg cattgatggt
    15721 cggaagctgg caccattatt acaatcagaa ctggcagtct ggcgttaacg tactggcaca
    15781 agctaaagcc tttatggacg taccaccacc gcagggaacg gatgaattcg gcaatctgca
    15841 attgccattg cttaacccgg tacgcgatgc caccctggcc tatggtgatt atcgcgatca
    15901 cggttttctg gcggatatgg gattgtacca gggcgcccgc gtagggccgt atgtggagca
    15961 aacctacatt cagcttcttg agcagcgtta tctcccctcg ttaatgaacg gcctgatccg
    16021 ggatctaaac attgccccgc cagagagcga agaaaagctc gctgtgctgc gcgtagtgcg
    16081 catgatggaa gacaaaagtg ggcgcaacaa cgaggcggta aaacagtaca tggcgcggcg
    16141 ctggagcaat gaatttcacg gccagcgcga tattcaggcg caactgatgg cgcatctgga
    16201 ctatgcgctg gagcacaccg actggcacgc gcagcgccag agcggtgaca gcgatgctgt
    16261 cagccgctgg accccctatg ataaaccgat cattaatgcg cagcaggaac tgagcaagct
    16321 gcccatatac cagcgtgtct accagaccct gcgcaccaaa gcattaagcg tgttgcccgc
    16381 cgatttgaat ttgcgcgacc aggttggtcc caccttcgac aacgtgttcg tcgccggtaa
    16441 tgatgaaaaa ctggtgatcc cgcagttcct cacccgctat ggactgcaaa gctattttgt
    16501 caaacagcgt gagggcctcg ttgagctgac cgcgctggat tcgtgggtac tgaacctgac
    16561 gcaaagcgtc gcctacagcg aggccgaccg tgaagagatc cagcgccata tcactgaaca
    16621 gtacctcagt gactataccg ctacatggcg tgccggaatg gataatctca acgtccgtga
    16681 ctatgagacc atgccggcgc tgaccgacgc gctggagcag attatcagcg gtgatcagcc
    16741 attcctgcgt gctctgacgg cgctgcgcga taatacacac gcgctgacgc tctccggcaa
    16801 actggacgat aaggcgaagg aagcggcgat aaatgagatg gattaccgcc tgttatcccg
    16861 gctggggcat gagttcgcgc cggaaaatag cgcactggag gagcaaaagg acaaggcgag
    16921 tactctacag gccgtgtatc agcaactgac cgagctgcac cgttacctgc tggcgatcca
    16981 gaactcgcca gtgtctggga aatcggcgct gaaagcagta cagctacggc tggatcaaaa
    17041 cagcagcgat ccaatcttcg ccacccgtca gatggcaaaa accctgcctg cgcctcttaa
    17101 ccgctgggta ggtaagctgg cggatcaggc ctggcatgta gtgatggtgg aagccgttcg
    17161 ttacatggaa gtggactggc gcgacaatgt agtgaaaccc ttcaacgagc agcttgccga
    17221 taactatccg tttaatccgc gcgccacaca ggatgcctca ctggattcgt ttgaacgttt
    17281 ctttaaaccg gatggcattc tggacaattt ctacaagaac aacctgcgcc tgttccttga
    17341 aaacgatctg acctttggcg acgacggcag aatgttaatc cgtgaagata tccggcagca
    17401 actggatact gcgcagaaaa tccgcgacat cttcttcagc cagcagaacg ggctgggcgc
    17461 acagtttgcc gtggaaaccg tatcgctttc cggcaataag cggcgcagcg tacttaacct
    17521 ggacggccag ttagtggact acagccaggg acgcaactac accgcccatc tggtctggcc
    17581 gaacaacatg cgtgaaggca atgaaagcaa gctgacgctg attggcacca gcggcagagc
    17641 accgcgcagt atcgcgttca gtggaccgtg ggcgcagttc cgcctgttcg gcgcgggcca
    17701 gttgaccaat gtgaccagtg acacctttaa cgtgcgcttt aacgtggacg gcggcgcaat
    17761 ggtttaccag gtgcatgtgg ataccgaaga taacccgttc accggcggtc tgttcagcct
    17821 gttccgttta ccggatacgt tgtattaagg ggatagtgat gagcaattac gcactgacac
    17881 aaactatcgt taccggcagc gatccgcgcg ccctgccgga gttcagcgcc atccgcgaag
    17941 aaattaacaa agctaaccac ccgtcacagc cggagctgaa ctggaaactg gtggaatcgc
    18001 tggcgctgtc gatttttaag gccaacgggg tggatttgca taccgccacc tactatacgc
    18061 tggcacgtac acgtaatcag ggactggcag ggttctgtga aggcgcggaa ctgctggctg
    18121 cgatgatcat ccacgaatgg gataaattct ggccgcagag tggtccggcg cggactgaga
    18181 tgctggactg gttcaacacc cgcaccggga atatcctgcg ccagcaggtt tccttttccg
    18241 aaaatgatct ttcgttgctc taccgcacgg aacgggcttt gcagcttatc tgcgacaaac
    18301 tccagcaagt ggaactgaag cgccagccgc gcgtggaaaa tctgctttat tttgtgcaga
    18361 acacacgcaa gcggtttgag ccgcagccca gaaaccgtac tgatacggcg gcacagacca
    18421 tggtcagaac gctggtctat gcgcctgaag gcacggcttc cgccacagcg gaaaccatgc
    18481 cccccctacc cgacctgccg gaaatgcggg tgggggtgcg cggtgtggca gataacgccg
    18541 acaaagcaaa acagggcgac acggtaaaag gttttgttgc cggtgccgcc tgtactgccg
    18601 ttatcgcatc tgcactctgg tggtggcagg cgtatccgat gcagcagcag cttacacagg
    18661 tcagggatac cacacaggga gccaccaccg tctggttggc atccccggta ctgaaagaat
    18721 atgaacagta cctgcaacag cttctgaatg ccccgccact acagccgctg gaaaccggaa
    18781 tgcagatgat gcgcaccgca gacaccctct ggccggaaag cctgcaacag caggaagcca
    18841 gcaggatgtg gagcaacacg ctgagaaacc gggcgcaagc cagtccgcag atgaagggct
    18901 ggcaacaagc gcggcagaat ttgcgggatt ttgcagattt gatgatgaag aaggagacgg
    18961 agaaacaggg tttcacccta tcgtatatca aaacggtgac ctggcaggcc gaacggttac
    19021 tgaatcaaga aacaccactg gagtacctgc tgacgcagta ccaggagacc cgtacacaga
    19081 agcaggacac acaggcgttg gaaaaagaga tcaacgaacg gctggacggg ctgttaagcc
    19141 gctggctgct gctgaaaaat accggacagg atatggcaac ggataacaga accgaacctg
    19201 tccatccaac tcattgagat taaggagaat aacaatggca aatcccgtct atctgacact
    19261 gaacggagaa cttcaggggc tgatttcatc cggatgctcg tcaatgccgt ccatcggtaa
    19321 caaagcccag attgcacatc aggatcagat catggtgacg tcactgtccc atggtctgag
    19381 ccgtgcgcag aatgtcaacc atcaggagtt gaccatcacg aaaccggtgg acaaatcctc
    19441 ccctctgctg ggcaaggcca tttcagagaa tgaatgtctg acctgtgatt ttgtattcta
    19501 ccgcaccaac cgctttggaa ttaatgaacc gtactacaag ctgaaactgg ctaacgcccg
    19561 gctctcaaac atcggcctta ccattcccca taccattaac gacagtcctg ggcaggcgga
    19621 agagtctgtg tcatttacct atgagagcat caattgggag cacagcgtcg ccggaaccag
    19681 tgcatacagc ctttggagtg aacggatttt ctaagttacc agggagtctt tcatctgcat
    19741 t
//
LOCUS       DAZFRG010000076        19731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFRG010000076 DAZFRG010000000
VERSION     DAZFRG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.076"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..697
                     /gene="yfaZ"
                     /locus_tag="ACHKUC_002110"
     CDS             155..697
                     /gene="yfaZ"
                     /locus_tag="ACHKUC_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO6618365.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            797..1999
                     /gene="yfaY"
                     /locus_tag="ACHKUC_002111"
     CDS             797..1999
                     /gene="yfaY"
                     /locus_tag="ACHKUC_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO6618366.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2219..3001
                     /gene="yfaX"
                     /locus_tag="ACHKUC_002112"
     CDS             2219..3001
                     /gene="yfaX"
                     /locus_tag="ACHKUC_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6618367.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPEAVQKSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3016..4221
                     /gene="rhmD"
                     /locus_tag="ACHKUC_002113"
     CDS             3016..4221
                     /gene="rhmD"
                     /locus_tag="ACHKUC_002113"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO6618368.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYAIKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSENGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4278..5567
                     /gene="yfaV"
                     /locus_tag="ACHKUC_002114"
     CDS             4278..5567
                     /gene="yfaV"
                     /locus_tag="ACHKUC_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6618369.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5585..6388
                     /gene="yfaU"
                     /locus_tag="ACHKUC_002115"
     CDS             5585..6388
                     /gene="yfaU"
                     /locus_tag="ACHKUC_002115"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO6618370.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6429..6662)
                     /locus_tag="ACHKUC_002116"
                     /pseudo
     CDS             complement(6429..6662)
                     /locus_tag="ACHKUC_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6663..7574)
                     /locus_tag="ACHKUC_002117"
     CDS             complement(6663..7574)
                     /locus_tag="ACHKUC_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO6618371.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTITERLRQEGHQIGWQEGKIEGLHEQAIKIAL
                     RMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7767..8957)
                     /gene="glpC"
                     /locus_tag="ACHKUC_002118"
     CDS             complement(7767..8957)
                     /gene="glpC"
                     /locus_tag="ACHKUC_002118"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO6618372.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8954..10213)
                     /gene="glpB"
                     /locus_tag="ACHKUC_002119"
     CDS             complement(8954..10213)
                     /gene="glpB"
                     /locus_tag="ACHKUC_002119"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO6618373.1"
                     /translation="MHFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10203..11831)
                     /gene="glpA"
                     /locus_tag="ACHKUC_002120"
     CDS             complement(10203..11831)
                     /gene="glpA"
                     /locus_tag="ACHKUC_002120"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO6618374.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGINAEAIEPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12104..13462
                     /gene="glpT"
                     /locus_tag="ACHKUC_002121"
     CDS             12104..13462
                     /gene="glpT"
                     /locus_tag="ACHKUC_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO6618375.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13467..14543
                     /gene="glpQ"
                     /locus_tag="ACHKUC_002122"
     CDS             13467..14543
                     /gene="glpQ"
                     /locus_tag="ACHKUC_002122"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6618376.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPANIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14746..15396
                     /gene="inaA"
                     /locus_tag="ACHKUC_002123"
     CDS             14746..15396
                     /gene="inaA"
                     /locus_tag="ACHKUC_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO6618377.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKVEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15450..15704)
                     /gene="yfaE"
                     /locus_tag="ACHKUC_002124"
     CDS             complement(15450..15704)
                     /gene="yfaE"
                     /locus_tag="ACHKUC_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO6618378.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15704..16834)
                     /gene="nrdB"
                     /locus_tag="ACHKUC_002125"
     CDS             complement(15704..16834)
                     /gene="nrdB"
                     /locus_tag="ACHKUC_002125"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6618379.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17024..19309)
                     /gene="nrdA"
                     /locus_tag="ACHKUC_002126"
     CDS             complement(17024..19309)
                     /gene="nrdA"
                     /locus_tag="ACHKUC_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6618380.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPVIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4542 a         4875 c         5361 g         4953 t
ORIGIN      
        1 agtgtgttta ttttttcggc gtttaattat ttaagaagta tttagttaca ataagtgact
       61 agatcccttt tttcgctcat tgaatcctct gcgccaggca gtacaatccc gcagctttgg
      121 gaattttgat taattgcttg tgggaaagag gataatgaaa aaaattgcgc tagcaggtct
      181 ggccggaatg ctgttggttt ctgcatcggt caatgcaatg agcatcagcg gccaggcggg
      241 taaagaatac accaatattg gtgtcggttt tgggactgaa tcgacgggcc tggcgttaag
      301 cggtaactgg acacataacg acgacgacgg tgatgccgcg ggcgtggggc tggggttgaa
      361 tctgcctctc gggccattaa tggcgaccgt tggcggaaaa ggcgtttaca ccaacccgaa
      421 cgatggtgat gaaggttatg ccgcagcagt tggcggtggt ttgcagtggc aaattggtga
      481 cagcttccgc ttgtttggtg agtattacta ctctccggat tcgctctcca gcggaattaa
      541 aagttatgaa gaagcgaatg ctggcgcgcg ttacaccatt atgcgtccag tcagcattga
      601 ggctggctac cgttacctga acctggcagg taaaaatggc aatcgtgata acgccattgc
      661 tgatggtccg tatgttgggg taaatgccag cttctgacgc cttccggcgc ggcgttctgt
      721 cgcgccattc agtttcttac tacgccctgt ctccctttgc gttatagtga ttcgataaca
      781 aaagcaggag ataaccatgt taaaagtgga aatgttatcc accggggatg aagtgttaca
      841 cgggcaaatc gttgacacta acgctgcctg gctggccgat tttttctttc atcaggggtt
      901 gccattatct cgccgcaata cggtggggga taaccttgat gacttagtca ccattcttcg
      961 cgaacgtagt cagcacgccg atgtgctgat cgttaacggc gggctgggac cgaccagtga
     1021 tgatttaagc gcactcgccg ctgcgacagc aaaaggtgaa ggcctggtgc tgcatgaagc
     1081 ctggctcaaa gagatggaac gctatttcca cgaacgggga cgagtaatgg caccgagcaa
     1141 ccgtaaacaa gcggagttgc ctgccagtgc tgaatttatc aataacccgg taggcactgc
     1201 ctgtggtttt gccatacagc ttaatcgttg cctgatgttc tttacccccg gcgtaccgtc
     1261 agaatttaag gtgatggtcg agcacgaaat cctgccgcgc ctgcgcgagc gtttttcttt
     1321 accacagccg ccggtttgcc tgcgtttgac tacctttggt cgttcggaaa gcgatctggc
     1381 acaaagcctg gacactctac aactgccgcc gggcgtaaca atgggctatc gctcctcaat
     1441 gccgattatc gaactgaaac tcaccggacc ggcaagcgag caacaggcga tggaaaaact
     1501 gtggctggac gttaaacgtg ttgccggaca gagcgtgatt ttcgaaggca ccgaaggact
     1561 gcccgcgcag atcagtcgcg aattgcagag ccgccagttc agcctgacgc tgagcgagca
     1621 attcaccggt ggtttattgg ctttgcaact ttctcgcgca ggtgctccat tgctggcgtg
     1681 tgaagtggtt ccttcacagg aggaaaccct ggcgcaaacg gcgcactgga ttacagaacg
     1741 gcgggcgaac cattttgctg ggttggcact ggctgtttct ggtctggaag atgagcatct
     1801 caactttgca ctagccacgc cagacggcac tttcgctttg cgtgtacatt tcagtgccac
     1861 acgctacagt ctggctattc gccaggaagt ctgcgcaatg atggcgctta atatgctacg
     1921 ccgttggtta aacggccagg acatcgccag cgagcatggc tggattgagg ttgttgagtc
     1981 catgacctta tctgtctgat tccgccactc tgtgccggtt tacagcaccg gcacattccc
     2041 cctccggcaa aatttgtcca acctgttgta ctcaataaga aaaaatccgt gcgctgattc
     2101 acaaatttgt gcgtaatttc tccagtccgc tggacgagaa acgggatttg atgcgcttca
     2161 caattttact ccccctgagc aacgagactg attccagtat ctaagactgg agtccagtat
     2221 gttggaatca agcaaagttc ccgccctgac acgggctatt gatattctca atttgattgc
     2281 ccggattggc ccctgtagtg ctgcgacgat cattgacaca ttggggatcc ctaaaagcac
     2341 agcctatttg ctgcttaatg aactcaggcg tcagcgtttt cttagccttg atcaccagga
     2401 aaatttttgt ttgtggacca ggctggtgga gctttccggt catgcgttaa gcaagatgga
     2461 tctccgggaa ctggctcgac cgcgtttgac gcaactgatg gataccaccg ggctgttatg
     2521 tcacctgggg attattgata acggcagcgc ttattacatt ttgaaagtgg agtcatcagc
     2581 cactatcagc gtgcgttccc atgaaggaaa aagcctttcg ctttatcgct ccggaatagg
     2641 taaatgcctg cttgcctggc aacctgaagc tgtacagaaa agtattatcg aagagctggt
     2701 ctgggagcag atgacaccca ccaccattac ccatccacaa cggctctacg aggaactggc
     2761 gcgtattcgc cgtcagggct ggagctatga caacggcgaa gattatgccg atgtccgttg
     2821 tgtcgctgcg ccggtgttta acgccaataa cgagcttacc gctgcgattt cggtggtcgg
     2881 tacccgctta caaatcaatg aagaataccg tgattatctg gcgggcaagg ccattgcctg
     2941 cgccagggat atttcacgtt tgttgggatg gaaaagtccc tttgacttac aggcctcata
     3001 acaatggaga acatcatgac cctaccaaaa atcaaacagg ttcgcgcctg gtttactggc
     3061 ggtgcgacag cagaaaaagg cgctggcggc ggtgattatc acgaccaggg ggcgaatcac
     3121 tggattgatg atcatattgc caccccgatg agtaaatacc gcgattacga gcagtcacgc
     3181 cagtcatttg gcattaacgt tcttggcacg ttgattgttg aagttgaagc agaaaacggc
     3241 cagaccggat tcgctgtttc gacagccggt gaaatgggct gttttattgt cgaaaaacat
     3301 cttaaccgtt tcattgaggg taaatgcgtc agtgatatca aactgatcca cgatcaaatg
     3361 ctcaatgcca ccctgtatta ctccggctct ggtggcctgg tgatgaatac gatttcctgt
     3421 gtcgatctgg ctctgtggga tctgttcggc aaagtggtcg ggcttccggt ttataaactt
     3481 ttaggtggtg cagttcgtga tgaaattcag ttctacgcca caggtgcgcg tccggatctg
     3541 gcaaaagaga tgggctttat cggtggcaaa atgccgacgc actgggggcc acatgatggt
     3601 gatgcgggga tccgcaaaga tgccgctatg gtcgcggata tgcgtgaaaa atgcggtgag
     3661 gatttctggt taatgctcga ctgctggatg agtcaggacg tgaactatgc catcaaactg
     3721 gcccacgctt gcgcgcccta taacctgaaa tggatcgaag agtgcctgcc gccacagcag
     3781 tatgaaggtt atcgcgaact gaaacacaac gcaccagcgg ggatgatggt caccagcggt
     3841 gagcaccacg gcacactgca atctttccgt acgctttcag aaaacggtat cgacattatg
     3901 cagccggatg ttggctggtg cggtggctta accacgctgg tggaaattgc cgcaatcgcc
     3961 aaatccaggg ggcaactggt ggtgccacac ggttcgtctg tttactccca ccatgcggtg
     4021 atcaccttca ccaatacgcc attcagcgaa ttcctgatga ccagcccgga ttgttcaacg
     4081 atgcgtccgc aatttgatcc gattctgctt aatgagccag taccggtgaa tggtcgtatt
     4141 cataaatcag tgcttgataa acccggtttc ggcgtcgaac tcaatcgtga ctgcaatttg
     4201 aaacgcccct acagccacta atcactccgg gcgttgctgt gacaacggta acgcccacat
     4261 attctaagga gctttttatg agcaccgctt tgcttgacgc cgtggtgaag aaaaaccgcg
     4321 cgcgtttaat tccgtttatg ttggcgctgt atgtgctggc gtttctcgac cgttcgaata
     4381 ttggttttgc caaacagacc taccagattg ataccgggct gagtaatgaa gcttatgcgc
     4441 tgggagcagg cattttcttt gtggtatacg cgtttctggg ggttccggcg aatcttttga
     4501 tgcgcaaact gggggcaaga acctggattg gtacgacaac actgctgtgg ggatttcttt
     4561 cggcagccat ggcatgggcc gatactgaag cgaaatttct gattgttcgc actctgcttg
     4621 gtgctgcgga ggccgggttt ttccctggta tgatttatct cacctcgcaa tggtttccgc
     4681 agcgtaatcg cgccagcatt atggggctgt tctatatggg cgcaccgctg gcgttaacac
     4741 tgggatcacc gctttctggc gcgctgctgg agatgcatgg atttatgggg catcccggct
     4801 ggttctggat gtttgttatt gaaggattgt tggcagtcgg cgctggggta ttcacattct
     4861 tttggcttga tgacacaccg gagcaggcac gttttctgag taaacaagaa aaaacgttgc
     4921 ttatcaatca actggcaagt gaagaacaac agaaagtgac ttctcgattg agcgatgcgc
     4981 tgcgtaatgg ccgagtctgg caactggcga ttatctacct gaccattcag gtagcggttt
     5041 acggattaat tttcttcctg ccgacccagg ttgctgcatt gctggggaca aaagtgggct
     5101 ttacggcgtc ggtggtcacc gccattccgt gggttgcggc cttgtttgga acctggctta
     5161 ttccgcgcta ctccgataaa accggcgaac ggcgtaatgt cgcagcgctg acattactgg
     5221 cggcgggcat tggtattggt ctgtccgggc tgctttctcc agtactggcg atcgtagcgc
     5281 tgtgtgttgc agccatcggg tttattgccg tgcaaccggt gttctggacg atgccgacac
     5341 agcttctttc cggtacggcg ctggctgcgg ggattggttt tataaacctg tttggtgcag
     5401 tgggcgggtt tattgccccg atcctgcgcg tgaaagcaga aacgttattt tccagcgatg
     5461 cggcgggatt actgacgctg gcagcggtgg cggtcatcgg ttcgctgatt attttcactc
     5521 tgcgtgtaaa tcgcactgtt gcgcagaccg acgtggcaca tcattaaata gtttaaggaa
     5581 cacgatgaac gcattattaa ccaatccctt taaagaacgt ttacgcaagg gcgaagtgca
     5641 aattggtctg tggttaagct caacgactgc ctatatggca gaaattgccg ccacttccgg
     5701 ttatgactgg ttgctgattg acggggagca tgcgccaaac accattcagg atctttatca
     5761 ccagctacag gcggtagcgc cctatgccag ccaccccgtg atccgtccgg tggaaggcag
     5821 taagccgctg attaaacaag tcctggatat tggcgcgcaa actctactga tcccgatggt
     5881 cgatactgcc gatcaggcac gtcaggtggt gtctgccacg cgctatcctc cctacggtga
     5941 gcgtggtgtt ggggccagtg tggcacgggc tgcacgctgg ggacgcattg agaattacat
     6001 ggcgcaagtc aacgattcgc tttgtctgtt ggtgcaggtg gaaagtaaaa cagcactgga
     6061 taatctggac gaaatcctcg atgtcgaagg gattgatggc gtgtttattg gacctgcgga
     6121 tctttctgcg tcgttgggct acccggataa cgccgggcac ccggaagtgc agcgaattat
     6181 tgaaaccagc attcggcgga tccgtgctgc gggtaaagcg gctggttttc tggctgtggc
     6241 tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg ttggcgttga
     6301 cacgatgctc tacagcgatg ccttggatca acgactggcg atgtttaaat caggcaaaaa
     6361 tgggccacgc gtaaaaggta gttattgata tcaaaggccc atggggatcg gctgtgggcc
     6421 tgtgttaatt agtggttatt cgctgccaga tcggcttcgc ttagctgagt ggccgcgagc
     6481 acctggtcac gatcaatgcc ctgctccagc atgcgcaaag caattttaat ggcttgttca
     6541 tgcatgcctt cctgcaaacc ttctaattta ccttcttgcc agccttcaat cttaccttcc
     6601 tgccagccaa tttgatgtcc ttcctgccgt agccgttcag caatagtcat caatctctcc
     6661 ttttaatgac agttcggaat atcagcatca gtgagttggg ttgccgccag cacaatctca
     6721 cgttcaaagc cctgctccag catgcgcaag gcaattttaa tcgcttgttc atgcaaacct
     6781 tcaattttac cttcttgcca gccaatttga tgtccttcct gccgtagccg ttcagtaata
     6841 gtcattaatc tctccttttg tagtggtgaa cgttcggcaa tctcctgaat aaagcgggtg
     6901 aaacgggagg tatcgccgca ttgcagcaga taattaaaca gtgtttgtag ctgactgtca
     6961 ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcga ccataccgat taaatcgcgg
     7021 tcgcgaatat gcttttgaat cagttccagc agagctatac gccgatgttg catgatctca
     7081 tcgtcaggta cgatggtgat atccaccaac ggaaaagctt cggtgtacaa ctgccgggca
     7141 agttgcggat cgtcaaactc atccagccag ttgagtgagt acgggtaggg cgaggcttcg
     7201 ccatgataaa acagcaatgg caccaccagc ggaactctgt catagccttt atccaggtga
     7261 cgctgcatgg cggcagtggc atagcgcatt agccgaaaag ccatattctt ttctgcagag
     7321 ctttgatgtt caatcacgca gtagatatag ccgtcaccct cgctggtttc cacggaccac
     7381 aaaacatccg agtagtaagc gcgtaaactt ttttcaataa aactggtggg ttccaggcgt
     7441 aaggtttgca ggttgcaaag cttgcgcagc ggctctggta gatgtatttc cagaaaatcc
     7501 cgtgcggttt cgggtgtgaa cataaaggtt ttaaacaccg catcatgcgg ggaggaggtt
     7561 gttgattcgg tcatggttgt ccgtcacctg atatttatga tgacgcgaca ttacggttat
     7621 cccaggcctt tgacatctgc cagtttcagc ctttgcgaga cgttttcaga ggaattttca
     7681 ctgcgttgca acgcatacat tgggtacgga atcgtgtacg caaaaatgcc cggtaacaac
     7741 atgcttaccg ggcatcagaa aggagtttaa gccagcgcct gtgccagcag cgtaatcgga
     7801 tgttcgcagc gaagacttgt ggacatctca atctgccatt tacaggtttc gcagtcggtg
     7861 accaccagat ctgcgccgct ctcttctatc tggcggaaca gtggtgcgcc gatggcttgt
     7921 gaagtggggt agttctcttt tttgaaaccg taagttcccg caataccgca gcactgggaa
     7981 tccagcaccg ttaactcaag ccccgggatt ttacgcaaca gctccagggt gtagagcgtc
     8041 cagcccattt tttccatatg gcacggagtg tgataaacca ctttcagcgg cagcggtttc
     8101 agcggtaacg ttttgccttc gtccagcttg cgccacagcc agcgggtcgc cagttcgata
     8161 tgatcgcgca agcctttgtt gtcgacattc agcacttccg ggtattcgtc gcgcagggca
     8221 aatgtacacg ttgaggaggt ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg
     8281 atcgactcta cattcgtaat tgcctgtttg cgtgctttat cggtaaaacc gttggcgatc
     8341 agcggtacgc cgcagcattt ttctttgctg agcagttgta cgccggtacc catcgcgttg
     8401 agcactttaa ttaaatcttt gcctagctgc ggatggttgt agttaacgaa gcagccgtga
     8461 aagaaagcga cctggtcttt atactgcgct tgctgagccg ccacgctgcg ataccagcga
     8521 cggaacgtac cgaaggagta tttcggtagc gtgcgacggt ggtctatttt taacgctgca
     8581 tcaagcaact gccgcaccgg tttcagcgag gtagcggtgt tgacgatggg tgcgaacggc
     8641 gtggaaacgc tacccatcag gtcggtatga ctcaacacaa aattacgcag cgacgggcgt
     8701 gtggtgtcat atttcgcccg cgcgcgctgg ataatatcgc caatcttcac atcggacggg
     8761 caggcgactt cacaacgttt gcagttgatg caatatttca gcgcctcgtc atacagtgcg
     8821 ccatctttta aacgcagacg ctcgccatcc ggcccggctt gttttggccc cggataaccc
     8881 ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg
     8941 aagctggtgt cattcattgt tggcctcctg cgcgttgggc aatctgttga gcggcatgta
     9001 aagcactgac ggcacaaaca ccgccgccgc atccctgggc gatgggatca aatccgccca
     9061 gcaccgaacc gatggcaaac aggttctcaa tggtttgccc cgcctgcgac gggcgtagcg
     9121 cctcatcagt ggttacaccg aactgctgcc acggctgcgg cgcaaaaaaa tcgcccttat
     9181 accattcacc ccgcgtagcg gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc
     9241 cgtcacgttc tgccaccagt ccgccgctaa agaaactgcc gctcgcaagc accgcaaatc
     9301 gtgggcgcag cgggatatcg gcgtgattgc gcgtccagat ttcattcact acgccatttt
     9361 tacaggtcac ttttttcact tcatcgcccg gcatccacac gccgccctgg cgcacaaact
     9421 ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga aggcggcagc gttggcaaaa
     9481 gcatcaatga acaaggtagt ttttcattca accaatgcca cagtttgtca tcggccaggc
     9541 cgaagcaggc gggcatcagg atcatttcgc aggtattggc gaccggaata agtgcatcaa
     9601 gtaacaacgg ccagttttct tcattatcaa ggaagcgggc aatattcacc gcccggaact
     9661 cggtggcgtt attgcgcagc acatccagtt ccggcagttc aatttcagcg gtttcaactt
     9721 taagatccaa ttcgcgcaat gaagccgccg caagatgcgc ctgaaaatcc atcaggccgc
     9781 taattcccac tacacatatt ttcttcgcgg gcagcggcca gacagggact tctggcgaac
     9841 ttagccaggt tgagcgcaga gtgcctagcg gtgtaatccg ctggtgagcc agctctacgc
     9901 tgccctgcaa ttgcgcaccg ctttcagcga ttaatgcctg cgcctggcaa gcgagatcga
     9961 gcacgcgttg tggcccgaga agagtgtaag gatgggcagg tgcctgctga cgcaaagatt
    10021 ccagtccact gtgaatgtct gtcaccggtt gaccatccgg cagatggctc agcaaatcca
    10081 gcgatccaga tgagaaatgc agcgcgcttt gaccacgagt gacaatggca cagcgcaggc
    10141 cgtgtttttg cagttgcagg ccacagagta atccggcgag gccgccgccc ataatgacag
    10201 tatcaaagtg catctttctg ctccttctcc agaccacaca atccctgata aacccagcgg
    10261 gtaaattcgc tttcgcgcag tgcatctccc caggcgatgg gttgcacgcc tttccagcgt
    10321 tcgttaagga aggtggaaag ttgctcgata gattgcgcgg acgtcgtgac gttaaaacgt
    10381 tgcagcagtc cggcagcgcg gcaggcgcag agctcgccct ggcaggtgcc catccccaca
    10441 cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta aattttctac cgcgtactgc
    10501 acttcacccg cagttaccgc ttcgcactca cataccaggc tacggtgcag acggccttcg
    10561 ctcagccagg caggcgtgcg atcgccatga cgataaaccg cagaaccgcg cagcggggca
    10621 ggcagggaga tgactttacg caaggtaact tcagcgggtt cctgtgaacc aggaagtgcc
    10681 agatcggcgg tcgtacaggg gcgcgtgttt cccagtttgc ggcatacggc gtcggtagcc
    10741 cattccgcca tcagccgata ggtcatcagt ttgccaccgg tgatggtgat aaacccgtcc
    10801 agaccatcgc gttcagcatg gtcgagcagc acgatgccac ggctgacgtt acgcccgctc
    10861 ggatcgtcat cgctggcaac cagcgggcgt acgccagagt aggcccgcaa aatgcgcgtt
    10921 ttcgccatca cgggggccag tttttcccct tcacgcagca gaatatcaac ttcttctgcc
    10981 gtcactcgat tatcgtcaat ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc
    11041 gaaatggtat cgccaggcac cagaatatcg gcgtcggaag gtttgcggca gcggttgatc
    11101 acatgctgat taatgcggtg atccatgatc agcagcgatc ctttcgccgg gaacatgcga
    11161 atgcgcagat cggcatattc ggcaatgtgt tgcccccaga tcccggcggc attaactacg
    11221 acaggcgcat gaagtgcctg aatttcgccg gtgagatggt tacgtacacg aacaccgcac
    11281 accgtcgcgc cttcacgaat cagccccgtg acttcatgag cggtaaggat aacggcaccg
    11341 tgttctttgg catccagcat gtttgctgct gtcagacgaa atggatcaac ggtgccatcc
    11401 ggaactttca ccgcgccaat cagtgccggg ttaacggcag gttcgataat gcgcgcttgc
    11461 tgcggctcaa tggcttctgc gttgatccct gcttcttcgc aggcgcggat aaaagtggcc
    11521 tggaaggaga ggtcatcttc cggcagggtg ataaacaggc cgttggttgg ttctacgcag
    11581 tgacgtgcaa tgcgtttcag aatctggttt tcactaatac attcgcgggc cgattccgca
    11641 tcggttaccg catagcgcgc accgctgtgc agcaggccgt ggttacgccc ggtggcaccg
    11701 gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg
    11761 gcaatcccgg ctcccgttgc gccaccgcca atgataatca cgtcacttga ttgcgagtcg
    11821 cgagttttca ttgttatccc tctgaagttc gttttttacc atttagccat agtaaaaaca
    11881 tgaattgttt gatttcgcgc atattcgctc acaattcgaa agtgaaacgt gatttcatgc
    11941 gtcattctga acatttcaac catcgtattt aataatgtgt gcggtaattc acatttaatt
    12001 tatgaatgtt ttcttaacat cgcggcaact caagaaacgg caggttctcc cactgaatca
    12061 ggctgttaat cataaataag accacgggcc acggaggcta tcaatgttga gtatttttaa
    12121 accagcgcca cacaaagcgc gcttacctgc cgcggagatc gatccgactt atcgtcgatt
    12181 gcgctggcaa attttcctgg ggatattctt tggctatgct gcttactatt tggttcgtaa
    12241 gaactttgcg ctcgctatgc cttatctggt tgagcaggga ttctcacgcg gtgatttagg
    12301 ttttgccctt tcggggatct cgattgctta tggattttcg aaattcatca tgggttcggt
    12361 atcggatcgc tcgaatccgc gcgttttcct gcccgcaggt ttgattctgg cggcggcagt
    12421 gatgttgttt atgggctttg tgccatgggc gacgtcgagc attgcggtga tgtttgtact
    12481 gttgttcctc tgcggttggt tccaggggat ggggtggccg ccgtgtggtc gtactatggt
    12541 gcactggtgg tcgcagaaag aacgtggcgg tattgtgtca gtgtggaact gtgcgcacaa
    12601 cgtcggtggt ggtattccgc cgctgctgtt cctgctgggg atggcctggt tcaatgactg
    12661 gcacgcggcg ctctatatgc ccgctttctg tgccattctg gtggcactgt tcgcctttgc
    12721 gatgatgcgc gataccccgc aatcctgtgg cctgccgccg atcgaagagt acaaaaatga
    12781 ttatccggac gactataacg aaaaagcgga acaggagctg accgcgaagc agatcttcat
    12841 gcagtacgtg ctgccgaaca aactgctgtg gtatatcgcc atcgccaacg tgttcgttta
    12901 tctgctgcgt tacggcatcc tcgactggtc accgacttat ctgaaagagg ttaagcattt
    12961 cgcgctggat aaatcctcct gggcctactt tctttatgaa tatgcaggta ttccgggcac
    13021 cctgctgtgc ggctggatgt cggataaagt ctttcgtggt aaccgtgggg caaccggcgt
    13081 cttctttatg acactggtga ccatcgcgac tatcgtttac tggatgaacc cggcaggtaa
    13141 tccaaccgtc gatatgattt gtatgattgt tatcggcttc ctgatctatg gccctgtgat
    13201 gctgatcggt ctacatgcgc tggaactggc accgaaaaaa gcggcaggta cggcagcggg
    13261 ttttaccggg ctgtttggtt acctcggcgg ttcggtggcg gcgagcgcga ttgttggcta
    13321 caccgtggac ttcttcggct gggatggcgg ctttatggta atgattggcg gcagcattct
    13381 ggcggttatc ttgttgattg ttgtgatgat tggcgaaaaa cgtcgccatg aacaattact
    13441 gcaaaaacgc aacggaggct aatggcatga aattgacgct gaaaaacctt agcatggcga
    13501 tcatgatgag cactatagtc atgggaagca gtgcaatggc ggcggacagc aacgaaaaaa
    13561 tagtcatcgc ccatcgcggt gccagtggat atttgccgga gcatacgctg ccagcaaaag
    13621 cgatggcgta tgcgcaggga gcggattatc tggaacagga tttggtgatg accaaagacg
    13681 accatctggt tgttctgcat gaccattatc tcgatcgtgt tactgatgtt gccgatcgtt
    13741 tcccggatcg ggcgcgcaaa gacggtcgtt actacgcgat agatttcacg ctggatgaaa
    13801 ttaagtcgct gaaatttacc gaaggtttcg atattgaaaa cggtaaaaaa gtgcagactt
    13861 atccggggcg tttcccaatg ggtaaatctg acttccgggt gcacaccttt gaagaagaga
    13921 ttgaatttgt tcaggggtta aaccactcta ccgggaaaaa tatcggtatc tatccagaaa
    13981 ttaaagcgcc gtggttccat catcaggaag ggaaggatat tgcggcaaaa acgctggaag
    14041 tgctgaagaa atatggttac accggtaagg acgacaaagt ttatttgcaa tgttttgatg
    14101 ctgatgagct gaagcgtatt aagaatgtgc tggaacccaa aatgggtatg gatctcaatc
    14161 tggtacagct gattgcctat accgactgga atgaaacgca gcagaaacag ccggacggaa
    14221 gctgggttaa ttacaattac gactggatgt ttaagccggg tgccatgaaa caggtggcgg
    14281 aatatgctga tggtattggt ccggattacc atatgttgat tgaggagaca tcgcagccgg
    14341 ctaatatcaa actcactggc atggtgcaag atgctcagca gaataaactg gtagtgcatc
    14401 cttataccgt gcggtcagat aaactgcctg aatacactac tgatgtgaat cagttatatg
    14461 atgctctgta taacaaagcg ggtgtaaatg ggctatttac tgatttccct gataaggcag
    14521 taaaattcct taataaagag taaattaact accggatggc acttatgtgc tatccgatac
    14581 cactcttcat taaagtctgt gggttatcct taattgcaat tattttgagc gagatgactt
    14641 gttcccggca taagatactc gtcaatacaa cacgtattat taagattttc ctcagctaaa
    14701 catctatagt cattgtcgtt aataaaaaac gacaggcgat agggtatggc agtttcagca
    14761 aagcacgacg aatttaatca ctggtgggca acagagggcg actgggttga agagcctaac
    14821 tatcgccgta atggtatgag cggggtgcaa tgcgtcgagc gcaacggcaa aaagctgtat
    14881 gtaaagcgca tgacgcatca tctgtttcat tccgtacgtt atccgttcgg ccgaccaacg
    14941 attgtccgtg aggttgcggt aattaaagaa ctggaacggg ccggtgtcat cgtgccgaag
    15001 atcgtttttg gtgaagcggt gaaagttgag ggtgaatggc gcgcgttgct ggtgactgaa
    15061 gatatggcgg ggttcatcag cattgctgac tggtatgccc ggcatgcagt atcgccttat
    15121 tctgacgaag tacggcaagc catgttgaaa gcagtggcgc tggcgtttaa gaaaatgcat
    15181 agcgttaatc gtcagcatgg ctgttgttat gttcgccata tctatgtgaa aacagaaggt
    15241 aaggcagaag ctggatttct tgacctggag aaaagtcgtc gccgcttgcg tcgtgataaa
    15301 gcgattaatc atgacttccg ccagctggaa aaatatctgg agccgatccc caaagcggac
    15361 tgggagcagg tgaaagcata ttactacgct atgtaatcgt tgtaggccag ataagacgta
    15421 taagcgtcgt atctggcgtt aaaaccaatt cacatctcga tttcaatatc gccttttgcc
    15481 cggcaacaac agggcaaaat ttcccccggc tgaataaaag ctaacggttc ggcaatccag
    15541 tccacctggc ctgcgaccag tcgggtgcga caggagccgc agtaaccttc gcggcactgg
    15601 tactcaaccg ccacattgtg ggattccagc gccgccagaa gggaagggtg ttcatcctgg
    15661 cacagcagtt gtgtgccagt aatgcgcagg gtaacgcggg ccatcagagc tggaagttac
    15721 tcaaatcgtc ggtgtccact tccgcgtcaa tctgcccgac cagataagaa ctgacttcca
    15781 cttcctgcgg agcaacctgc acgttatcag acaccagcca ggtgttgatc cacgggatcg
    15841 ggttggagcg cgtctggaac ggcagatcca aaccgactgc ctgcatacgg atattggtga
    15901 tgtattcaac gtactggcag agaatgtctt tattcagacc aatcatcgaa ccatcacgga
    15961 acagataatc cgcccagtct ttctcttgct gtgccgcctg gacaaacagg tcatagcact
    16021 cctgcttaca ttcttcggca atttccgcca tctccggatc atccgcgccg ctgcgcagca
    16081 gattcagcat atgctgggtg ccggtcaggt gcagggcttc gtcgcgggca atcaggcgaa
    16141 taattttggc gttaccttcc atcagctcac gttcagcgaa tgcgaaagag caggcaaagc
    16201 tgacgtagaa acggatcgct tccagcgcgt taacgctcat caggcagaga tacagttttt
    16261 tcttcagctc gcgcaggcta acggttacgg ttttaccgtt aacggtgtgg gtgccttcgc
    16321 ccagcagatg ccagtagctg gtcatttcga tcagttcatc gtaatagctg gagatccctt
    16381 ccgcacgttt ctggatctgc tcgttggtga cgatatcgtc aaagacaact gacggatcgt
    16441 taacgatatt acggatgata tgggtatagg agcgggaatg aatcgtttct gagaacgccc
    16501 aggtttctac ccaggtttcc agttccggga tcgagatcag cggcagcagt gccacgttcg
    16561 ggctgcgacc ctgaatggaa tccagcagcg tctgatattt cagattgctg ataaagatgt
    16621 gtttttcgtg ttcaggcagt gcctggtaat cgatacggtc gcgggagacg tcaacttctt
    16681 ccggacgcca gaagaaagag agctgctttt cgatcagctt ttcgaagatg tcatattttt
    16741 gctgatcgta gcgagccacg ttgaccggct gaccaaagaa cattggttct ttgagctgat
    16801 catttttcgt ctgtgaaaag gtggtatatg ccatgaatgt gtcctgttag gagttttatg
    16861 ccggatgcgc ttcgcttatc cggcctacaa accgcatcgt aggctgggta gggcgttcat
    16921 gccgcatctg ggcatccagt gccggatgcg cttcgcttat ccggcctaca aattggaccc
    16981 gtaggccggg tagggcgctc acgccgcatc cggcatctca atatcagatc ttacatgcgc
    17041 cgctttcgca gccatcgtcc tggattgacg gcaccagatc gtcttgtgcg tcttctgcac
    17101 cgtcacgggt gttctgataa tacagcgttt taaccccgaa tttgtaggcg gtgagcaggt
    17161 ctttcagcag ttgctgcatc ggcacttttc ctgacgggaa gcgtgacgga tcgtagttgg
    17221 tgttagcaga gatcgactga tcgataaatt tctgcatgat acccaccagt tgcagatagc
    17281 catcgttacc cggcatttcc cacagcagtt cataggcgtc gtgcagatgc tcgtagtccg
    17341 gcaccacctg gcgcagaata ccgtctttcg acgctttgat gctgacgtaa ccacgcggcg
    17401 gttcaatacc gttagtggcg ttagagatct gcgaagaggt ctctgacggc atcagagcag
    17461 aaagcgtgga gttacgcagg ccgtgcgttt tgattgactc acgcagtgct tcccagtcgt
    17521 aatgcagcgg ctcattagcg atggcatcca gatctttctt ataggtatcg atcggcagga
    17581 tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc gccttgctct ttcgccagct
    17641 cattagacgc tttcagcagg taatactgaa tggcttcgaa ggttttatgc gtcaggttgt
    17701 tggcgctgcc gtcggagtag cgtttaccgt gcttcgccag gtagtaagcg aagttgatca
    17761 caccaatacc cagcgtacga cgacccatcg ctccacgttt ggcggccggg atcgggtaat
    17821 cctgataatc cagcagcgcg tcgagtgcgc gtactgccag aattgccagc tcttccagtt
    17881 catccaggct gttaattgcg cccaggttga aagcagacag cgtacacagc gcgatttcac
    17941 cgttctcgtc gttgacgtcg ttcagcggtt tggtcggcag ggcgatttcc aggcacaggt
    18001 tagactggcg cactggcgcg atgaccggat caaacgggct atgggtattg cagtggtcaa
    18061 cgttctgaat atagatacga ccggtagacg cacgttcctg catcatcagc gagaacagct
    18121 caacggcttt cacacgttgc ttgcggatgc tgtcgtcttt ctcatatttg gtatacaggc
    18181 gttcaaactc ttcctgatcg gcgaagaacg cgtcatacag ccccggtacg tcggacgggc
    18241 tgaacagagt gatatcttca cctttcagca gacgggtata catcagtttg ttgatttgaa
    18301 ccccgtagtc catatgacgc acgcggttgc cttccacacc acggttgttt ttcaacacca
    18361 gcaggctttc cacttccaga tgccacatcg ggtagaacag cgttgccgca ccgccgcgca
    18421 caccgccctg agagcaggat ttcaccgcgg tctggaaatg tttgtagaac ggaatacaac
    18481 cggtatggaa cgcttcgcca ccgcgaatcg ggctacccag cgcacgaata cgcccggcgt
    18541 tgatgccgat cccggcacgc tgggaaacgt atttaacaat cgcgctggag gtggcgttaa
    18601 tggaatccag gctgtcgccg cactcgatca gcacgcatga gctgaactga cgagtcgggg
    18661 tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt
    18721 aaaaacgctt cacgtattgc aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg
    18781 caaccagaat ataaaggaac tgggcgcttt cgtagatttc gccggtcacg cggttctgta
    18841 ccagatattt gccttccagc tgcttaacgg cagcataaga gaaggtcata tcacggtcgt
    18901 gatcgataaa cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc agcagatgat
    18961 tatcgtattt gcccatctcg accattttca ccacatggtc gtacagtgca ggcggctcaa
    19021 actgaccgta ggctttttta cgcaggtgga agatcgccag acgtgcggcg agatactgat
    19081 aatccggcgc atcacgggag atcaggtctg cggcagcctt gataatggtt tcgtggatgt
    19141 cagaggtctt gataccgtca taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg
    19201 aaacgttatg cagtccttct gccgcccaat ccagaacgcg atggattttg tcgagattga
    19261 tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt ctgattcatg tatgtcgtac
    19321 ctgtttttca atagcttcat gcagactacc tgtagtgagg gaagctgcta agaacactat
    19381 atataggggg tatgtttggg attcactgca agatagtgtg aaactggccc tcttgcaagt
    19441 gcataacttt gtggataact caggaaggaa aaagtggctt ccgcgcacct taggtcagac
    19501 aaggtgtccg ggaaagtcaa tgggaagaga aaaatttgtt aaaaataacc gttcggtgaa
    19561 ttcttgaacg gtaaaatagg ataggtaaat tgatatagat ggtttattaa tcttgttgct
    19621 aatgattgcc aaaattgatc taggcacaag tcgtcacata atctgatgta tcagttgtgg
    19681 tcaatatata agcgagtgta agcgattgtt gagtcggaaa tggttgtttt a
//
LOCUS       DAZFRG010000077        19640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFRG010000077 DAZFRG010000000
VERSION     DAZFRG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.077"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHKUC_002127"
     CDS             complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHKUC_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO6618381.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1572..2777)
                     /gene="hemX"
                     /locus_tag="ACHKUC_002128"
     CDS             complement(1572..2777)
                     /gene="hemX"
                     /locus_tag="ACHKUC_002128"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO6618382.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     APAAAPQGE"
     gene            complement(2799..3539)
                     /gene="hemD"
                     /locus_tag="ACHKUC_002129"
     CDS             complement(2799..3539)
                     /gene="hemD"
                     /locus_tag="ACHKUC_002129"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO6618383.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIRDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3536..4477)
                     /gene="hemC"
                     /locus_tag="ACHKUC_002130"
     CDS             complement(3536..4477)
                     /gene="hemC"
                     /locus_tag="ACHKUC_002130"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO6618384.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4864..7410
                     /gene="cyaA"
                     /locus_tag="ACHKUC_002131"
     CDS             4864..7410
                     /gene="cyaA"
                     /locus_tag="ACHKUC_002131"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO6618385.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(7450..7770)
                     /gene="cyaY"
                     /locus_tag="ACHKUC_002132"
     CDS             complement(7450..7770)
                     /gene="cyaY"
                     /locus_tag="ACHKUC_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO6618386.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7818..9146)
                     /locus_tag="ACHKUC_002133"
     CDS             complement(7818..9146)
                     /locus_tag="ACHKUC_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO6618387.1"
                     /translation="MNGQIITVALILLGHIFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGAHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSQWIDLQGIEPEQFHLITDNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDSPDLVIDHSFNRGKQHAYFIHQLRSRKSVQIIPTNNGPLTKNDRFAYDDRNVYTWT
                     DTEVRITPSPCPAKTRVREENVHELHNRDIIIPLTDESCQNAADEQTLKP"
     gene            complement(9143..10612)
                     /locus_tag="ACHKUC_002134"
     CDS             complement(9143..10612)
                     /locus_tag="ACHKUC_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO6618388.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLLAPFGPWDTLARTQTKIMINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            10801..11004
                     /gene="yifL"
                     /locus_tag="ACHKUC_002135"
     CDS             10801..11004
                     /gene="yifL"
                     /locus_tag="ACHKUC_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6618389.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11041..11865
                     /gene="dapF"
                     /locus_tag="ACHKUC_002136"
     CDS             11041..11865
                     /gene="dapF"
                     /locus_tag="ACHKUC_002136"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO6618390.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPDLIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11862..12569
                     /gene="yigA"
                     /locus_tag="ACHKUC_002137"
     CDS             11862..12569
                     /gene="yigA"
                     /locus_tag="ACHKUC_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO6618391.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            12566..13462
                     /gene="xerC"
                     /locus_tag="ACHKUC_002138"
     CDS             12566..13462
                     /gene="xerC"
                     /locus_tag="ACHKUC_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO6618392.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13462..14178
                     /gene="yigB"
                     /locus_tag="ACHKUC_002139"
     CDS             13462..14178
                     /gene="yigB"
                     /locus_tag="ACHKUC_002139"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO6618393.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14262..16424
                     /gene="uvrD"
                     /locus_tag="ACHKUC_002140"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14262..16424
                     /gene="uvrD"
                     /locus_tag="ACHKUC_002140"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO6618394.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16469..17371)
                     /locus_tag="ACHKUC_002141"
     CDS             complement(16469..17371)
                     /locus_tag="ACHKUC_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO6618395.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTERKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17454..18218)
                     /gene="yigE"
                     /locus_tag="ACHKUC_002142"
     CDS             complement(17454..18218)
                     /gene="yigE"
                     /locus_tag="ACHKUC_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO6618396.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQIERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18380..18436
                     /gene="ysgD"
                     /locus_tag="ACHKUC_002143"
     CDS             18380..18436
                     /gene="ysgD"
                     /locus_tag="ACHKUC_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO6618397.1"
                     /translation="MDTPSRYWLIILSSRINS"
     gene            18588..19538
                     /gene="corA"
                     /locus_tag="ACHKUC_002144"
     CDS             18588..19538
                     /gene="corA"
                     /locus_tag="ACHKUC_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO6618398.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4504 a         5089 c         5196 g         4851 t
ORIGIN      
        1 cgcaggagaa atattcactc aggaagttat tactcaggaa gtaaagagga ttacagaatt
       61 atctcataac aagtgttaag ggatgttatt tcccggttct ctgtggcata ataaaagagt
      121 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      181 agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat cagacaccat
      241 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      301 ccgtaataag agcaggcgtt tttttatata tcagaaaggc cccggaggtg cttgcctccg
      361 ggtgagaaag agctactgtg gcgggttgtt ctgcaacgtt aacatcaaac cgtcgcgacg
      421 catcgctgcg gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      481 atcgtaggcg tccggacgtt gtttcagcgc tgcgcggaag gcgagcgatg cttcctgcca
      541 ttctccgtgt ttcatcagcg actgacccag tgtgctccac aacagcgggc gatcgcccac
      601 gtttttgatc tgttggcgca gcactttttc cagctgttcc gggttattgg ttttcagacg
      661 cgggatcggc agcagcaggc gatcgtcata ctggcgtttc aggccgtcga tgataatttg
      721 ctgggcagta tcatgatcgt cacactcaat aagatgttcc gccattgcca cctgcaaggc
      781 cacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcactacc
      841 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
      901 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagcgaact
      961 ccatgcacca gtgcggatat acgcctgttc cgccagacgt aatacttccg gatggcgtgg
     1021 cgtaacttcc agcagcttat ccacgccgtg acgtgctgca tggttttcat tacgggccag
     1081 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctccgctgc
     1141 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggctgc
     1201 cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca tcagcttttc
     1261 aacttgctga taatcgcctt ccgccagttt cagcagcgcc tgttcggtct gcttacgtgc
     1321 acggcgacgc ttacgtccga caaaccaccc acgggtgtgc gcgcctgtgc ggaagattcg
     1381 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa taatcgcgag
     1441 gcccgtgacg ctagtttcaa tattgtagtt gtcggtctgg atcagcacgt aaccctgatg
     1501 gccggcaatc atcgggccaa ccacgatccc cgcaatcagc aacacaaaga gcaataacac
     1561 ttttagcatg gttattctcc ttgcggcgcg gctgccggag catcagcttg cggtgcaggt
     1621 gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg ttgtccctgc tgccggttgt
     1681 gccagcaggt tacgcacgcg ggtctgcatc aatttttcca gcatcgcctg gctttgcagg
     1741 gtttccggaa gatccatcga gatattttgc tggcttaact ggtccacctc gtcgaggaac
     1801 gctttggtgg tggcatcatc agtatcgtag taagcacgta cccaggtgga gacgttctcc
     1861 agcgcctggc gataagtctc ttcctggtga cgcggtacag cttgtgctgc gaccagcagg
     1921 cgagagcgaa tattttcgcg cagatagata tcctgatttg gcgctaacag cggtacggcg
     1981 gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa agttctgcca gcttttttgc
     2041 agattgatac gccattcgct gatggaactg gaaagctctt caccgtcaga atccatcggc
     2101 gaaccatcgc tgtcattatc ggccagacgc agattatcta cctgatttga aagctgatta
     2161 agcttaagga tgataccgtc ataatccacc tgcgataccg cagaaaggct ggcgatatca
     2221 tcggtaattg cccggcgaac ggtaatcaga ctcgggtcat tcatatccgc caggctggcg
     2281 tctgcacttt tcagcaacgc tgcagcggtc gtgacgtcct gatcgctcca cagcttccgt
     2341 ccggcgagtt tcaccagaaa atcggcctgg gccagcagcc aggttttagc atcgctgccg
     2401 gaaatggtgg cgaccttttg ttggacttca tccagctgtt ttgccagcgt ttcttgctga
     2461 cgattcgcct gctcaagctg tacagcttgt tgcttgataa tgccttccag ctcggctttt
     2521 tggctctcct gggctttttg cagtgccgta agttggttag ccagggcatc gctggtggca
     2581 gtctgattga cggcctgttg tttaccccag ccatacaaac cgacgcccgc cgccagagca
     2641 atagcgatag ccaccgcgct gagaatcaat gcggtattgt tcttactctt tttttctgtt
     2701 gcgacaggtt gtgacgtggt gtccacggcc tccctggtct cttcaaccac ggcggaggtt
     2761 ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt attgtaatgc ccgtaaaagc
     2821 gcatcgttat cagcgttatc ggcgacctta atgtcttgcc agcccagttc ccgggcgagt
     2881 ttcgccaaac gctcactgac gactaatagt cgacagcgta gtaaccagtg ctcacgatac
     2941 cattgtggga tcagcgacca gagttgctgc aacatttcac cgctggtaac aacgaccgtc
     3001 gtcacctcgc gggattgcca gcgcatcgct tcttctgcac catcgtaatg gattgcgcat
     3061 cgttgataac attcacaaaa agtgacctca gcaccgcgcg ccgtcaggga atccctaatt
     3121 agctcacgac cgccattgcc acgtaatatc agcgcacgtt tgcccgcaat attttgtaat
     3181 tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct gcgggtagag aatcttctgt
     3241 ccgcttacgg tatgtagtgc cagcgcggtg gtgcgtccaa tggcgaaata agtaggtagt
     3301 cggggccatt tacgatcttg ctgatgcagc tgtgattggg caaaagcaac cgcgtgttgc
     3361 gagagggcaa acaacagatc gctctctccc agcgctgcca gttgatcagc aagttgcggt
     3421 aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg ccccagtgtg
     3481 cgcagacggc tcactaactc ttctcctgcg ggagacgggc gggtgaccag gatactcatg
     3541 ccggggcgtc tccgttatag acttcagcga ggatctcgcg cgcgccgtta ttcagtagct
     3601 cttctgccag cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg cgttcaccgc
     3661 gaataatctg cgaaccgtcc ggcgcgccaa ccaacgcacg cagccagatt tcgccatcaa
     3721 taagctcggc gtagctacca attggcacct gacatccgcc ttcgagacgg gtattcatgg
     3781 cgcgttcggc ggtaacgcgc agtgcagttt cgtggtgatt cagcgcggcg agcagctcgc
     3841 gagtgcgtgc atcatcaagg cggcattcaa tgcccaccgc accttgccct accgccggaa
     3901 gagaaatctc gggtggcaac gcggcgcgaa tgcgggactc cagacctaaa cgttttagtc
     3961 cggctacggc aagaatgatg gcatcatatt cgccgttatc cagtttgctc agacgagtgc
     4021 cgacgttgcc gcgcagggag cggataatca gatccgggcg gcgttcagcc agttggcact
     4081 ggcgacgtaa actggacgtc ccgacgatac tgcctgccgg taacgcatcc agactgtcat
     4141 agttattgga cacaaatgca tcgcgaggat cttcacgctc gcaaatagtg accagcccca
     4201 gaccttgcgg gaattcaacc ggcacatctt tcattgaatg tacggcgata tcggcgcgat
     4261 tttcgaggag cgcgacttcc agctctttca caaataagcc tttcccgcct acttttgcca
     4321 gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg taccagttca acgaccaggc
     4381 ccggatggct cgccatcaac ttgtctttga catagtgtgc ctgccagagt gcaagtgggc
     4441 tttggcgtgt ggcaattctt aaaacattgt ctaacatgct tgttaccgtc attatcatcc
     4501 gtggtccatc ctaacatcct tgccagagcg atgtcagtgt tgtggtgaaa cgtagacgcc
     4561 tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac aggcgctttg agccttcgta
     4621 ataaggaatt tacagagaat aaacggtgct acacttgtat gtagcgcatc tttctttacg
     4681 gtcaatcagc aaggtgttaa attgatcacg ttttagacca ttttttcgtc gtgaaactaa
     4741 aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat cccgaatgcc
     4801 gcgtgttacc gttgatgttg gcggaatcac agtcatgacg ggtagcaaat caggcgatac
     4861 gtcttgtacc tctatattga gactctgaaa cagagactgg atgccataaa tcaattgcgt
     4921 gtggatcgcg cgcttgctgc tatggggcct gcattccaac aggtctacag tctactgccg
     4981 acattgttgc actatcacca tccgctaatg ccgggttacc ttgatggtaa cgttcccaaa
     5041 ggcatttgcc tttacacgcc tgatgaaact caacgccact acctgaacga gcttgaactg
     5101 tatcgtggaa tgtcagtaca ggacccaccg aaaggtgagc ttccaattac tggtgtatac
     5161 accatgggca gcacctcgtc cgtagggcaa agttgttcct ctgacctgga tatctgggtc
     5221 tgtcatcaat cctggctcga tagcgaagag cgccagttgc tacaacgtaa atgtagtctg
     5281 ctggaaagct gggccgcctc gctgggtgtg gaagtcagct tcttcctgat tgatgaaaac
     5341 cgcttccgtc ataatgaaag cggcagcctg gggggcgaag attgtggctc cacccagcat
     5401 atactgctgc ttgacgaatt ttatcgtacc gccgtgcgtc tcgccggtaa gcgtattctg
     5461 tggaatatgg tgccgtgcga cgaagaagag cattacgacg actatgtgat gacgctttac
     5521 gcgcagggcg tgttgacgcc aaatgaatgg ctggatcttg gtggcttaag ctcgctttct
     5581 gctgaagagt actttggtgc cagcctttgg cagctctaca agagtatcga ttccccatac
     5641 aaagcggtgc tgaaaacact gctgctggaa gcctattcct gggaataccc gaacccacgt
     5701 ctgctggcga aagatatcaa acagcgtttg cacgacggcg aaatagtgtc gtttggtctc
     5761 gacccgtatt gcatgatgct ggagcgcgtc actgaatatc tgacggcgat cgaagatttt
     5821 actcgtctgg atttagttcg ccgatgcttc tatttaaaag tttgcgaaaa actcagccgt
     5881 gaacgcgcct gcgtaggctg gcgtcgtgaa gtgttaagcc agttggtgaa agagtgggaa
     5941 tgggacgatt ctcgtctggc aatgcttgat aaccgcgcta actggaagat agatcaggtg
     6001 cgtgaggcgc acaacgagtt gctcgacgcg atgatgcaga gctaccgcaa tctgatccgc
     6061 tttgcgcgtc gcaataacct tagcgtttcc gccagcccgc aggatatcgg tgttctgaca
     6121 cgtaaactgt acgccgcgtt tgaagcgtta ccgggtaaag tgacgctggt aaacccgcag
     6181 atttcacccg atctctcgga accgaatctg acctttattt atgtgccgcc gggccgggct
     6241 aaccgttcag gttggtatct gtataaccgc gcgccaaata ttgagtcgat catcagccat
     6301 cagccgctgg aatataaccg ttacctgaat aaactggtgg cgtgggcatg gtttaacggc
     6361 ctgctgacct cgcgcacccg cttgtatatt aaaggtaacg gcattgtcga tttgcctaag
     6421 ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc tgcgcttacc tgcaccgaca
     6481 ccgaaggcgc tctacagccc gtgtgagatc cgccatctgg cgattatcgt taacctggaa
     6541 tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt ttgatttccg taagctggat
     6601 gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta gcgttgacct gctgtatcgc
     6661 aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg agcaatcgat gatcgaagcc
     6721 ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac cgccagatag cgtggaagtc
     6781 ttctgttata gccagcatct gcgcggctta atccgtactc gcgtgcagca actggtttct
     6841 gagtgtattg aactgcgtct ttccagcacc cgccaggaaa ccgggcgctt caaggcgctg
     6901 cgcgtctccg gtcaaacctg gggcttgttc ttcgaacggc tgaatgtctc ggtacagaaa
     6961 ctggaaaacg ccatcgagtt ttatggcgct atttcgcata acaaactgca cggcctgtca
     7021 gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg tggacggctt tgccagcgaa
     7081 gggatcatcc agtttttctt cgaagaaacg caagacgaga atggctttaa tatctacatt
     7141 ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa agaggaactg
     7201 gtgcgtgacg tcagtcgctt ctactcgtct tcgcacgacc gtttcaccta cggctcaagc
     7261 ttcatcaact tcaacctgcc gcagttctat cagattgtga aggttgatgg tcgtgaacag
     7321 gtgattccgt ttcgcaccaa atctatcggt aacatgccac ctgccaatca ggagcatgac
     7381 gcgccgctgt tgcagcagta tttttcgtga tgaacgtgcc ggaaagcgag gcttatccgg
     7441 catgcaatct tagcggaaac taactgtttc acccgcctgc tgcgtcgccg cctgttccag
     7501 caaatcccag aaggtttcac cgctacgatc gcaaatccac tcgtcgcctt tcagatcaaa
     7561 atggtagcca ccctgtttgg ttgccagcca cacctggtgc agtggttcct ggcggttgat
     7621 aatgattttg ctgccgttct caaaggtaat ggttagtaca ccgccgttga tttcacagtc
     7681 gatatcgctg tcgccatccc agtcgtccag gcgttcttca atggtcagcc agagttgatc
     7741 agccaggcga tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag tgatggcggc
     7801 agtatagcgg catggggtca gggcttcaaa gtttgctcgt ctgctgcgtt ctggcatgat
     7861 tcatccgtca acggaataat aatgtctctg ttgtgaagtt catgcacatt ctcttctctg
     7921 acgcgtgttt tcgccgggca gggggagggc gtaatcctta cctctgtatc ggtccatgta
     7981 taaacattgc gatcgtcata agcaaagcga tcgtttttag ttagcgggcc gttgttagtt
     8041 ggaataattt gcacgctctt gcgtgaccgt aattggtgga taaagtaagc atgctgtttg
     8101 ccgcgattaa atgagtgatc aatgaccagg tctggcgaat ccagcgggat tatttcaagc
     8161 tggtcttcac caaaagtcga taattttgtt acatacagcc gatctttata ctgcgcaata
     8221 ttgtcagtaa tcagatgaaa ttgttcaggt tcaatacctt gtaggtctat ccactggctc
     8281 cactgacggt cgggcgtgag ctgtttacgc cagcgaacgc gactttcccc tacttcaaaa
     8341 gagacgtcat catctacggg tatcgctttg ccagataatt gaccaaaggg ataacgatgc
     8401 agacccttat tgtcgcgaaa aaccagcagt gaatgaggta tccagcgaat tatctcaaat
     8461 gagtctgcat tcgccgtaag gtgagcgcca tttaaataaa tattctgccc actgcggata
     8521 agcagatcat tggaatacaa atttctgttt acactcataa gacgtggatt aataccccac
     8581 catctttttt ctaatacagt aacattactg ctgctcccct gacgtcgacc ggacaagtag
     8641 agatttttac tatccaccag cgtggtagag ttaccttcga tagcttttag tgtcgccaga
     8701 tcaacacgtg acgcgccgct attactttcg gtgcgctgtc cgtcaaaata gaggctatat
     8761 ttatcgaccg cgaagcggcc catagcctga aagctggcaa tatcgacagt gggtgttccc
     8821 ggtggattgc taacgatttt gccacgccag agaataacgc ggccgtctgt cacccaatgg
     8881 tattcctcat tttttatgct tatttcatcg gtgggtatat agccttccac caactcccgc
     8941 tccccggata tatttgtcac aaggcgcaaa tcggctctgt tcatcttcgg aaattgggca
     9001 atgattttct catcctcccc ataaccttta ccgtaagaca taacaccgtg atcattgtaa
     9061 tagtcgaacc ctatggcctg tacagcaggc gtcagaaata tgtgccccaa caggatgagt
     9121 gcaacagtga ttatttgacc attcattttg tcgcctcatt gcgcggggca ggaataaatt
     9181 cgccgtcatc ggtaattttt ccgtccagat atcccgtttt gtaatttacc ccgtagccat
     9241 atcccgaatg ttgttccttc cagattaagc gaacttgttt cggatccgca gtgcggtatc
     9301 gccagagctg cgcgccatca aagctgtaaa cgtagcggtt atcgcgggca taccatctgc
     9361 gcaaaaaggg atggtcatta agatgggctt cgcgttcatc cggagaacgg ttatccagta
     9421 agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga atggtatggc cacccttcgg
     9481 cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa acgtttgttg ataataagat
     9541 tgggatcatc aagtttcacg atctccaact tgcggtcacc tgactctgtt tcgatgatgg
     9601 tataaagcga gtcctgatac tgggcgacag catcactgat ggggtgaaac tgttccggat
     9661 cgagtcccgg taattcttcc tgtttacagt caggcccctg acgccagaac actttctttt
     9721 ccagcagaga aaaatccaga cagtgctttg ttagatcctc atcccacgcc agattttcct
     9781 tgtcgaggac tttacgctgt aacccgtttt tatcacgcca ggtcaagagt gagccgggca
     9841 tccagcgcac gacggaaaag gtgtccgggt cggcatccag ttgttctccg ttgatcatga
     9901 ttttcgtctg tgtgcgggct agcgtatccc aggggccaaa tggggcaaga aggcagggat
     9961 taaatgctgt agagaattta ccgcgctgat cccatgactt ttgagccagt accgtaaagg
    10021 tgtcgggatc atcaaggcgc tgaccatttg cgtaaagata gtgcttgtca cggagaatca
    10081 aacctagcca gtctggtttc cattgcgggt taaattctac ctgacgcaac gttcggcggt
    10141 ttagttgctg gttgctatcg gtgcgtttgc cttcgtaata gaggctgtac ttatctgcgg
    10201 caaaatcacc ccatgcctga aatgacgaga tatcgactgg cggtgtgcct ggagggtttt
    10261 gcacaatctc gccagcgtag agaatagcgc gaccatcgct atagtaagag taatggggaa
    10321 gagttgcgtt tgcgccgctg tagacaacgt ctgtcatgag attggtagct gggggcaccg
    10381 tttcccaatc ctccagtttg gttgggtcaa ttagcaaatt cggtaacaga cgcagacgct
    10441 tgtagttaat ctgcggtagc gaaagattgg tcttttcata agggcgcagg tacaccacgc
    10501 cttttccctg tttctggtaa atattatgtt ccggctcatc aagccgacat ccccatgccg
    10561 acgataagaa caacgaacaa gacaataaac aaaccacatt gcgatagtgc ataaagccat
    10621 cctggcgcga ggtgccgatc acgaaactac cagcaaaaca taaatcgcca cgtgtaagcg
    10681 ttatactcgc agcaattcct cacttttcag acttcataaa gagtcgctaa acgcttgctt
    10741 ttacgccttc tcctgcgatg atagaaagca gaaagcgatg aactttacag gcaatccata
    10801 atgaaaaacg tgtttaaggc actcactgta ttacttactc tcttcagcct gacgggctgc
    10861 ggtctgaaag gtccgctcta tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa
    10921 ccggtagaga cgcaaacgca atccacggtg ccggataaaa acgaccgcgc cactggcgat
    10981 ggtccatccc aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg
    11041 atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg
    11101 cagaatgtct ttttttcacc ggatctgatt cgtcgcctgg cggatcggca cctgggggta
    11161 gggtttgacc aattgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat
    11221 cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gcaacggcgc gcgctgcttt
    11281 gcccgttttg tgcgcctgaa agggctgacc aataagcgcg atatccgcgt cagcaccgcc
    11341 aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa
    11401 cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt
    11461 atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc
    11521 gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa
    11581 agccacgagc gttttccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag
    11641 catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc
    11701 gcgtgtgcgg cggtggcggt agggattcag caaggtttgc tggccgaaga agtacgcgtg
    11761 gaactccctg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg
    11821 actggcccgg cggtacatgt ctacgacgga tttattcatc tatgaagcaa ccaggggaag
    11881 aactgcagga aacactcacg gagcttgatg accgggcggt tgtcgattat ctgattaaaa
    11941 atcctgagtt ttttatccgc aatgcgcgcg cagtagaagc gatacgtgtg ccgcatccgg
    12001 tacgcggcac cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat catattcatg
    12061 ttctggaaga gaacatggcg ttgttgatgg aacaggctat cgccaacgaa ggcctgtttt
    12121 atcgtctact ctacctgcag cgcagtctca ccgccgccag tagtctcgac gatatgctga
    12181 tgcgtttcca tcgctgggcg cgtgatttag ggctggcagg tgcgagtctg cgtctgtttc
    12241 cggatcgctg gcgcttaggt gcgccgtcga accacactca tctggcatta agccgtcagt
    12301 ctttcgaacc gctgcgtatt cagcgtctgg ggcaggaaca gcactatctg gggccgctta
    12361 acggaccgga gcttctggtg gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt
    12421 cgatgctggg aagcgatgct gatttgggtg ttgtgctgtt taccagtcgc gatgccagtc
    12481 actatcaaca agggcaggga acgcagttac ttcatgaaat tgcactgatg ttgccggagc
    12541 ttctggcgcg ttggattgaa cgcgtatgac cgatttacac accgatgtag aacgctatct
    12601 acgttatctg agcgtggagc gccagcttag cccgataacc ctgcttaact accagcgaca
    12661 gcttgaggcg atcatccatt ttgccagcga aaacggcctg caaagctggc agcaatgtga
    12721 tgtgacgatg gtgcgtaatt ttgctgtacg cagtcgccgt aaagggctgg gagctgcaag
    12781 tctggcgtta cggctttctg cgctacgtag cttttttgac tggctggtca gccagaacga
    12841 actcaaagct aacccggcga aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa
    12901 aaacatcgac gtcgatgata tgaatcggct gctggatatt gatatcaacg atcccctcgc
    12961 cgtgcgcgat cgggcaatgc tggaagtgat gtacggcgcg ggtctgcgtc tttccgagct
    13021 ggtggggctc gatatcaaac acctcgacct ggagtctggc gaagtgtggg tgatgggaaa
    13081 aggcagcaaa gagcgccgcc tgccgattgg tcgcaacgcc gtggcgtgga ttgagcactg
    13141 gcttgatttg cgcgacctgt ttggtagtga agacgacgcg ctttttctgt cgaaactggg
    13201 caaacgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca taaaacaagg
    13261 gctgaataat cacgttcacc cgcataaatt acgtcactcg ttcgctacgc atatgctgga
    13321 gtcgagcggc gatcttcgtg gtgtgcagga gctgctgggt catgccaacc tctccaccac
    13381 gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc
    13441 acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat ctcggcgctc
    13501 acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg caccgagcga
    13561 gaggcgctta cctttgtgca aaattatcat ccggcgctgc gcagcttcca gaatgaagat
    13621 ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca cgacgtgacg
    13681 cgttggcgtt ttcgttcgat tgaacaagcg atgctcgacg ccgggctgag tgccgaagaa
    13741 gccagtgcag gcgcacacgc agcaatgatt aactttgcca aatggcgcag ccgaatcgac
    13801 gtcccgcagc aaactcacga caccttaaaa cagctggcga agaaatggcc gctggtggcg
    13861 atcaccaacg gtaacgccca gccggagctg tttggtctgg gggattattt tgagtttgtg
    13921 ctgcgcgctg gcccgcacgg gcgttcgaaa ccgttcagcg atatgtactt cctggcggcg
    13981 gaaaaactca acgtgccgat cggcgagatc ttacatgtag gtgacgatct taccaccgac
    14041 gtgggtgggg caattcgcag cggaatgcag gcctgttgga tcagaccgga aaatggcgat
    14101 ctgatgcaaa cctgggacag ccgtttactg ccgcatctgg aaatttcccg gttggcatct
    14161 ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg gcggagggcg
    14221 ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt tcttacctgc
    14281 tcgacagcct taatgataaa cagcgcgaag cggtggccgc gccacgcagc aaccttctgg
    14341 tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc gcctggctga
    14401 tgagcgtgga aaactgctcg ccgtattcga ttatggcggt gacgtttacc aacaaagcgg
    14461 cggcggagat gcgtcatcgt atcgggcaac tgatgggcac cagccagggc ggcatgtggg
    14521 tcggcacctt ccacgggctg gcgcaccgcc tgctgcgtgc gcaccatatg gacgccaacc
    14581 tgccgcagga tttccagatc ctcgacagcg aggaccagct acgcctgctc aaacgcctaa
    14641 tcaaggcgat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg tggtacatca
    14701 acagccagaa agatgagggg ctgcgtccgc atcatattca gagctacggt aatccggtgg
    14761 agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg
    14821 ttgattttgc tgaattgctg cttcgtgccc acgaattatg gctcaacaaa ccgcatatcc
    14881 ttcaacatta ccgcgaacgt ttcaccaata ttctggtgga tgaattccag gacaccaaca
    14941 acattcagta tgcgtggatc cgactgctgg caggcgatac tggcaaagtg atgatcgttg
    15001 gcgatgacga ccagtcaatc tacggctggc gcggagcgca ggtggagaat attcagcgtt
    15061 tcctcaatga tttccccggt gctgaaacta tccgtctgga gcagaactac cgctctacca
    15121 gcaatattct gagcgccgct aacgccctga ttgaaaacaa taacgggcgt ctgggtaaaa
    15181 aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct tttaacgaac
    15241 tcgacgaagc gcgttttgtg gttaaccgca tcaaaacctg gcaggacaac ggcggggcgc
    15301 ttgccgagtg cgccattctc taccgcagca acgcccagtc gcgtgtgctg gaagaggcgt
    15361 tattgcaggc gagtatgccg tatcgtattt acggcgggat gcgcttcttc gaacgccagg
    15421 aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgcaacgac gacgcggcct
    15481 ttgagcgtgt ggttaatacg ccaacgcggg gtattggtga ccggacgctg gacgtggtac
    15541 gtcagacatc gcgagatcgc cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg
    15601 aaaaagccct cgccggacgt gctgccagcg ccttacagcg gtttatggag ctgatcgacg
    15661 ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg gtaattaaag
    15721 actccggcct gcgcaccatg tacgagcagg agaagggcga aaaaggtcag acgcgtatcg
    15781 aaaacttaga ggaactggtg acggcaacgc gccagttcag ctacaacgaa gaagacgaag
    15841 atttaatgcc gctgcaggca ttcctctccc atgcggcgct ggaagcgggc gaggggcagg
    15901 cggatacctg gcaggacgcg gtgcagttga tgacgctaca ctcggcgaaa ggtctggaat
    15961 tcccgcaggt gtttatcgtt ggtatggaag agggcatgtt cccaagccag atgtcgctgg
    16021 atgaaggcgg gcgtctggaa gaagaacgcc gtctggccta cgttggcgta acccgcgcga
    16081 tgcagaaact gacgctgacc tacgcggaaa cccgccgtct gtatggcaaa gaggtttacc
    16141 atcgcccgtc gcgctttatt ggcgagctgc cggaagagtg tgtggaagag gtgcgcctgc
    16201 gcgctacggt aagccgtccg gtcagccatc aacggatggg tacgccgatg gtcgagaacg
    16261 acagcggcta caagctcggc cagcgcgtac gccacgctaa gtttggtgaa ggcaccatcg
    16321 tcaatatgga aggcagcggt gaacatagcc gtttgcaggt ggcattccag ggccagggta
    16381 ttaaatggct ggtggcggct tacgcccggt tggagacggt gtgacgttgc ggaatgcggt
    16441 gcgccacacc gcattccgca tcagcacctt acttagcgca cttcttcaca tcaatattcc
    16501 agtcagcacc agacgcaacc tgatgtttct gcgcgaaatt atccatattc agcgccacgg
    16561 aaacattcaa taagctgttc agatacacca tcggctggcc ttccggcaca tcgccaaagc
    16621 ttgcgacata cggcattttc cctgcgtatt tttgctggga accttcggaa atggtcacac
    16681 acaacgtgtc gttgagtttg attcctgcct gattcagcaa ctcgtcgctg atattgctcc
    16741 agacgttgcc gtactgaata tcgagaatcg ggatattgcc cttcacttca ccttttgttg
    16801 ctttggcttt ctgataagaa agttcgacaa cttttgcagg aagctctggc cccacctgct
    16861 cgaacgtgat cgccccggaa gccagacgtg caccggtgta ggcatacacg tcacgaccgt
    16921 ggaaggtata tgacttttcg gaacctttca gacggttggt tttttcatcg atttgccgga
    16981 cgctctcaat acccagcgat tccgccacca acgtaagcgt accgttatct ggtgaaacga
    17041 agtactgacc gtttttggtt ttcagcacta ccgatttacg ctctgtacca acacctggat
    17101 cgaccacgct aacaaacacc gaaccttgcg gccagtaact ggcagtctga tacaggcggt
    17161 aagcgccttc ccagatgtta tacggtggaa tttcgtgtgt taaatcaaat attttaagat
    17221 tatggtcaac gccgaaagcg acccctttca tcgccgagac ggcgccatct ttcaggctaa
    17281 aatccgtttg taaaatcaaa gcgttatccg cataagcgga tgatgacccc agacaggtta
    17341 ataaaaataa tgttgcgagc gtttttttca tttttgtttc ccttcattta tctgatgatt
    17401 ttccagctgt gtagccagcc ccagtttgtc tggcctcctc gagtgtacaa tgtttaacct
    17461 ttctgctcga cggaaatcat ggtgacaaaa ggatagcgtt gccacgggat tgcctcgcct
    17521 ttcatataca tatgtgaaat tgtgccatca agataaagca gctgctcaac gttcagtttc
    17581 gctttggcat aacaggcaaa atcataaaaa tttgtcgcct gctggctcaa caaaaacacg
    17641 gcgttcccat gtttattaat cccaacaccg ttacgaattt tacgtgaggc gacgttggga
    17701 tgaatacgcg gattaattac accgttttcc atcaacaatg gccctgactg caccgcaaac
    17761 tgaatctctt tactggtttt aaaggcatcc agacgaacga tgccgacttt atctcccgcg
    17821 acataaaaca ctccgccagg acgaataaag aaattccctt cacctgaagc gagatttaac
    17881 gccaccttct gctgaccgtt ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag
    17941 atgccgccgt tcatcgccat ctgcacctga ccctgactat taatatccgc cagcagagca
    18001 tgtaacgttc cccacgcttc gccattggct ttttgccagt acattttcac ccgctctatt
    18061 tgaggattaa cggtatacgc ctgtacggtc agcgtcggat ctgagggtgc gcaatcatca
    18121 gcggcaacag caaacaaggg aagtaaggtg agggtgagaa aaatccgttt gagattcaag
    18181 gtgatcattc ctttaccaat gaatagccga tgcgccatta taggccctga atgtgggatt
    18241 tttttatcct gttagcgacc ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct
    18301 gtggtgttct gttgcgtgtt gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca
    18361 ctctaagagg acattcgcct tggacacacc cagtagatac tggctcatta tcctgtcatc
    18421 caggatcaac tcctaaggct atcccttttt gctgatagcc ttagcggttg tcagcgacct
    18481 caatttttcc cgtcgcgctg agtcaggctg tttaatggtc tgaaacccaa tttgtttctg
    18541 tgtgcccacc gaactgtccg atattttaag cattgggagt cccggtcatg ctgagcgcat
    18601 ttcaactgga aaataaccga ctgacccggc tggaagtcga agagtcacaa ccccttgtaa
    18661 atgcagtatg gattgatctt gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg
    18721 aacttggcca gagcctggca acccgcccgg aactggaaga catcgaagca tcggcacgtt
    18781 tctttgaaga cgatgacggc ctgcatattc actccttctt cttctttgaa gatgcggaag
    18841 atcacgccgg taactccact gtggcattta ccatccgtga tggtcgtctg tttactctgc
    18901 gtgagcgtga actgcccgct tttcgtctgt atcgtatgcg tgcccgtagc cagtcaatgg
    18961 tggacggtaa cgcctacgag ctgctgctgg atctgttcga aaccaaaatc gaacagttgg
    19021 cagatgaaat tgaaaatatc tacagcgacc tggagcagtt gagccgggta attatggaag
    19081 ggcatcaggg cgatgagtac gacgaggcgc tctccacgct ggcggaactg gaagatatcg
    19141 gctggaaagt tcgcctgtgt ctgatggata cccagcgcgc gctcaacttc ctggtacgta
    19201 aagcacgctt accgggtggg caactggagc aggcgcgtga aatcctgcga gatatcgaat
    19261 ccctgctgcc gcataacgaa tccctgttcc agaaggtgaa cttcctgatg caggcggcga
    19321 tgggctttat caatatcgag cagaaccgca tcatcaaaat cttctcggtg gtatccgtgg
    19381 tgttcctgcc accgacgctg gtcgcttcca gctacgggat gaactttgag tttatgccgg
    19441 aactgaagtg gagcttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg
    19501 caccgtatct gtactttaag cggaagaact ggttgtaata taaatgccgg gtaaagattg
    19561 ttctttatcc ggcttattgc tgaaaattgt ctttttcaaa gtcatgcggg ttgaacactc
    19621 gctctatctt attttcctgg
//
LOCUS       DAZFRG010000078        19454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFRG010000078 DAZFRG010000000
VERSION     DAZFRG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.078"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..317
                     /gene="bhsA"
                     /locus_tag="ACHKUC_002145"
                     /gene_synonym="ycfR"
     CDS             60..317
                     /gene="bhsA"
                     /locus_tag="ACHKUC_002145"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO6618399.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(399..1361)
                     /gene="ldtC"
                     /locus_tag="ACHKUC_002146"
     CDS             complement(399..1361)
                     /gene="ldtC"
                     /locus_tag="ACHKUC_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO6618400.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1504..4950)
                     /gene="mfd"
                     /locus_tag="ACHKUC_002147"
     CDS             complement(1504..4950)
                     /gene="mfd"
                     /locus_tag="ACHKUC_002147"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO6618401.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHNFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLINTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5078..6151)
                     /locus_tag="ACHKUC_002148"
     CDS             complement(5078..6151)
                     /locus_tag="ACHKUC_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO6618402.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAISALNQWLAPERNLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6412..7611
                     /gene="lolC"
                     /locus_tag="ACHKUC_002149"
     CDS             6412..7611
                     /gene="lolC"
                     /locus_tag="ACHKUC_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO6618403.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7604..8305
                     /gene="lolD"
                     /locus_tag="ACHKUC_002150"
     CDS             7604..8305
                     /gene="lolD"
                     /locus_tag="ACHKUC_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO6618404.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVLFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8305..9549
                     /gene="lolE"
                     /locus_tag="ACHKUC_002151"
     CDS             8305..9549
                     /gene="lolE"
                     /locus_tag="ACHKUC_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO6618405.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9578..10489
                     /gene="nagK"
                     /locus_tag="ACHKUC_002152"
     CDS             9578..10489
                     /gene="nagK"
                     /locus_tag="ACHKUC_002152"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO6618406.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10505..11326
                     /gene="cobB"
                     /locus_tag="ACHKUC_002153"
     CDS             10505..11326
                     /gene="cobB"
                     /locus_tag="ACHKUC_002153"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO6618407.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVCCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11464..12252)
                     /locus_tag="ACHKUC_002154"
     CDS             complement(11464..12252)
                     /locus_tag="ACHKUC_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO6618408.1"
                     /translation="MKKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12249..12710)
                     /locus_tag="ACHKUC_002155"
     CDS             complement(12249..12710)
                     /locus_tag="ACHKUC_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO6618409.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12768..13814)
                     /gene="potD"
                     /locus_tag="ACHKUC_002156"
     CDS             complement(12768..13814)
                     /gene="potD"
                     /locus_tag="ACHKUC_002156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO6618410.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(13811..14605)
                     /gene="potC"
                     /locus_tag="ACHKUC_002157"
     CDS             complement(13811..14605)
                     /gene="potC"
                     /locus_tag="ACHKUC_002157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO6618411.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14772..15890)
                     /locus_tag="ACHKUC_002158"
     CDS             complement(14772..15890)
                     /locus_tag="ACHKUC_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6618412.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            complement(15859..16128)
                     /locus_tag="ACHKUC_002159"
     CDS             complement(15859..16128)
                     /locus_tag="ACHKUC_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6618413.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIEPEIHTGDDDALKEILTHVTEATKKQH"
     gene            complement(16190..18628)
                     /locus_tag="ACHKUC_002160"
     CDS             complement(16190..18628)
                     /locus_tag="ACHKUC_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6618414.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSVQEKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHSSEKPVTHVAREEL
                     QALHIARAEELPAVTALAISHKTKLLDPLEIRDLHKLVRDTDKVFPNPGNSDLGLITA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTFDALD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLA
                     AECKTEEEKNDEDNPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTADEV
                     NKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQESEQND
                     QKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPDCGAVMGDATYQET
                     FDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHHVITS
                     TSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SVIWQKLIPSQADF"
     gene            complement(18721..18909)
                     /locus_tag="ACHKUC_002161"
     CDS             complement(18721..18909)
                     /locus_tag="ACHKUC_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6618415.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(18906..19094)
                     /gene="dicB"
                     /locus_tag="ACHKUC_002162"
     CDS             complement(18906..19094)
                     /gene="dicB"
                     /locus_tag="ACHKUC_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6618416.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
BASE COUNT         4597 a         4857 c         4955 g         5045 t
ORIGIN      
        1 tagttatcga tcgttaagta attgcttgcg acgtcattca tctgcataag gccactatta
       61 tgaaaaacgt aaaaaccctc atcgctgcgg cgattttaag ctccatgtca tttgccagct
      121 ttgcggctgt cgaagttcag tccacgccag aaggccaaca aaaagtcggt actatcagtg
      181 ctaacgcggg gacaaatctg ggatcgctgg aagagcagct ggcgcaaaaa gcggatgaga
      241 tgggcgcaaa atctttccgt attacttctg taaccggtcc gaataccctc catggaacag
      301 cagtaattta taaataagca ttaaccctca ttaatgcctg ctactgctga ttttttcccc
      361 gcgacatgct gtgtcgcggg gattttttta tccgagcttt acagagtttg tgggctcact
      421 tgatgacggc ggacatccac cggcatcccg gaacggacat ccatcacatg ttgcatcact
      481 tcagcgtcag tttgtgctgc atctttaaat gattgcattg cgctattcag cgtaattggc
      541 agcagctgcg gatcgtcatc aatcttctct gacagcggct gatgtacttc aaccagacgc
      601 gcaccgtttg gttcggcaga gactttaatc ggggtgttga tgatattcac tttggtgcct
      661 ggggtgacct ggctaaagag tgttttgata tcgtcatccc gcagacgaat acagccagaa
      721 cttacccgca tgccaatgcc gaaatcagcg ttcgtaccgt gaagcaaata aacgccgcca
      781 taggccgcca gacgaatcgc atgatggccc attgggttat ccggtccagc cggaactacc
      841 gcaggcaact caattccctg tgccttatac cgcgcgcgaa tgtttgccgt tggcgtccag
      901 gttgggtttg cacgcttgtc agaaacggtg gtcaccattg tcggtgtcag cgtgtcacca
      961 cctaactgac caatgccgat gggatatacg gttaccgaat tttttcccgg cggataataa
     1021 tagagacgca gctccgcaat gttgatcaca atgccttcgc gcggcgcgtc tggaagtagg
     1081 gtttgcaacg ggatcgttaa cacgctaccc gcgcgcggta cgtaaggatc aacgccgggg
     1141 ttagcctgta acagagcgag aaagccgacg ttatattttt tggcgatcgc ttccagagaa
     1201 ccaccgtcat tttccaccac atgaaatttg ttttcaccaa ccagacgact gcccgctgac
     1261 ggcagcggcc aggtgttggc ttttgccggt agcgccagcg ccacggcagc ggcgaacgta
     1321 aaaaacgtta gccagcgaga aaaacgcgtt ttgatcatca ccaaagtcca taataaatat
     1381 aaggttattg taataaaaag gtaagtctga attatggagg gtgacagtgt cggataatgc
     1441 agggaagtgc aaagaatttg taaatattgc agatgggggc gcagaaacgc ccccgattta
     1501 ccattaagcg atcgcgttct cttccagttc acgcataaac tggcgtaccc attcgatacg
     1561 cgttttccgc tcgctcaaat cctgaataaa cttcagacgc gtcggaccat caaggcggta
     1621 atgctgcggc tgtttttgca gcaaaccaat caaccaggcc ggattaacgt gattcttctc
     1681 ggcaaattcg atcacgccgc ctttctcatt accttccagc ttcctgatcc ccagtttctg
     1741 cgcttgctgg cgcagacggg caatatccag cagggtacgc gccggatccg gcagcaggcc
     1801 gaagcgatcg ataagctcga ctttgatctc ttccagttcg ttttccgttt tggcgctggc
     1861 aatacgtttg tagaacgaca gacgcgtgtt cacgtcaggg atgaaatcat ctggcaatag
     1921 cgacggcatc cgcagctcga cttctgtttg ctggctggtg agatcttcca gcgacggctc
     1981 gcgtccggct ttcagcgcat cgacggcgtt ttccagcaac tccatataca gtgagaaacc
     2041 gatggtttcc attgagccgc tttgttcttc gccaagcagt tcacctgcgc cgcggatctc
     2101 cagatcgtgc gttgccagcg caaaacctgc accgagatct tccagcgagg caatcgcttc
     2161 aagacgtttt tgcgcatcgg tagtcatcgc ttttggatgc ggcgtcagta gccatgcata
     2221 tgcctggtgg tgagaacgcc cgacgcgacc gcgcaactgg tgcaactgtg ccagaccgaa
     2281 gtgatccgcg cgttcaatga taatggtgtt ggcggtagga atgtcgatcc cggtttcgat
     2341 aatggtggta caaaccagca cgttgaaacg ttgatgatgg aaatcattca tcacccgttc
     2401 cagttcgcgc tcacgcattt gcccgtgacc gatggcaatc cgcgcttcag gtaccagttc
     2461 tgccagccgt tcggcggctt tctggatgtt ttccacatca ttgtagagat aataaacctg
     2521 tcctccgcgc aaaatttcac gcaggatcgc ctcccggacc accagactgt catactcacg
     2581 gacaaaggtt ttaactgcca gacgacgggc gggcggcgtg gcgataatcg acagatcgcg
     2641 cattccgctc attgccatat tcagcgtacg tgggatcggc gttgcggtaa gcgtcagaat
     2701 atccacgttc gcgcgcatcg ctttaatgcg ctctttatga cgcaccccga agcggtgttc
     2761 ttcatcgaca atcagcaggc ctaaatcttt aaacttgacg tcactttgca gcagtttgtg
     2821 cgtaccgatc agaatatcga ttttcccttc cgccacttcc gcaaggattt gcgtctgctc
     2881 tttggcgctg cggaaacggg agatcatttc gatacgtacc ggccagttgg cgaaacggtc
     2941 gcggaagttg tcgtaatgct gctgcgcgag aagggtggta ggcaccagca ccgccacctg
     3001 tttgtggtta tctaccgcca ggaaagcggc gcgcatcgcc acttctgttt taccaaagcc
     3061 aacatcgccg cacaccagac gatccattgc cagcggctga cacatgtcgc taagcaccgc
     3121 attaatggct tgagcctgat ccggcgtggt ttcaaacggg aagctgtcgc agaataactg
     3181 atactgctca cgatcgtgtt taaacgcgaa gccctctttg gcggcgcgtt gcgcgtagat
     3241 atccagcaat tccgccgcca catcacgcac tttttccgcc gctttctgcc gtgcgcgtga
     3301 ccaggcatcg ccaccaagtt tatgcagtgg tgcgttttct tctgcgccac ccgcgtaacg
     3361 gctaatcaga tgcagtgacg acaccggaac atacagtttg gcgtcgttgg cataggtgag
     3421 catcaaatac tcgccagtaa tgcctccagc ttcgagcgtg gtcattccgg cataacgacc
     3481 gacgccgtgc tccagatgga ccaccggctg accaatatgc agctccgcaa ggttacggat
     3541 cagtgtatcg gggttgatgg cgcggcgaga atcctgacga cgacgggcaa cgcgttcacc
     3601 gagcagatcg ctttcgcaaa tcagcgccag gttacgcatc gtatcgacaa aaccatgttc
     3661 ggcagcgcca atcatcagat aacgcccacg gtcgctggct tcatcaagac gcataatgcg
     3721 ttgcggagca attttaattc gcgcgagcag ttcacccagc gcttcacggc gaccttcact
     3781 ttctaccgag aacaccaccg gaccgtcgaa agtctcgagg aacttacgca gcgcatccag
     3841 cggcgctttt tgttgtgcct gaatggccag gtctggcagt ttctggaaac ctaaattggc
     3901 attcgctgct tttgtcggta aatgttcagt ttttagctgc acacgcggcc agtttttcag
     3961 ctctgaaaag agctcgtcta cccgcagcca gagcgattgt ggtggcaaca gtggacgcat
     4021 cggatcgacg ccgcgatttt caaaacgcgc cagcgtgtca gcctggaaac gttcggcact
     4081 gttttccaga tcgccagtat ttatcagcag cgtattggca ggaaaataac tgaacagtgg
     4141 cggcagcggt tcgctgaaga atagcggctg ccagtactcg atcccggcag gtaatgtgcc
     4201 tttactcact tgctggtaaa tatgttccgg atcgcgcttc acttcgaagg tatcgcgcca
     4261 ctggctgcgg aacagttcaa ttgccgcttt atcggtcgga aattcgtgcg cgggcagcag
     4321 attgatctct tctacttcct ccagcgtgcg ctggctatcg acgtcaaaca cccgcaggct
     4381 gtcgatttca tcatcaaaga aatcaagacg ataaggcagc tcactcccca tcgggaagag
     4441 atccagcaac gcgccgcgcg tggcgtattc gccgtgctcc atcacctggt caacatggcg
     4501 ataaccggcg ctgtccagtt gggttcgtaa tgcatctcgt gacaggcgct gacctttttt
     4561 catcaccagc gcatgaccgt ggagaaagtt gtgcgggcaa acgcgctgca taagcgtatt
     4621 caccggaaca atcagtacgc cacgctgcat ggtcggtagc tggtaaaggg tggaaaggcg
     4681 cgaggagata atgtcctgat gaggcgaaaa actgtcgtaa gggagggttt cccagtccgc
     4741 cagattcatc accatctgat cggtgaactg gctgatttca tcatgcaaac gcagagcatt
     4801 ttgcatatct ggtgcaatga gtaccaccgg accggcgtga cgttcggcaa tttccgctac
     4861 cagcgtcgcg caggcagcgc cggttaactc gcccagcaga cgctgctcac ccgctttgac
     4921 gggcagcgta taacgatatt gttcaggcat aacggttgtc agattctcgt taggatatgc
     4981 ctcaacatat gggggcatat ctctgataag caatgctgtt cattatccgt aatcgacatg
     5041 gtttagcaaa ttgaatcgcc ccggctgggg cgatggctta gcgggagtaa ctgacggccg
     5101 cgggcagaga aggcggggag aacaacagat cgctgaatgc gcgctgtgaa agttttctta
     5161 ccagcaagcc cgcaacagta cagatgaaca aactaacaaa ggggtaaacc agcaggagtg
     5221 tcagttcgac ttgcggcgac cagcgtgctg cgttcatttg cgcaagcaga gttaagctga
     5281 atatttcaac cagaatgcga tgggtggtgt agatagcaat ggtgttggaa ccaatcacat
     5341 tcagcaggct ggaggaacgc ataccgaaac gctgctcgta ctgataaaac agcttcatga
     5401 ttaccacaat cgataccagc gacaacagca gcgagatagt aaacaaccag gcaccgaccg
     5461 ccagaacggt cagcaaagaa gccatcagca gatggcggcg caacggcacc tctttaacac
     5521 aggtcattat tgttgcgcca aaccatgcgc caaggctgta ataaggcaaa ttgcggatca
     5581 cactgttcat tccccaccac ggcgtgggaa cgaaattaac cgccacactc atcagtacaa
     5641 acagaacaaa taacggcagg gccaggcggt taaaaatttt acatatcacg aaatagacaa
     5701 ttaaagcata cagataccac aagctggtgc tggcggtgat catcccgtgc aggaactcac
     5761 cggtagaatc ggcataagcg gcattggagg cattacttaa attgcgctca ggtgccagcc
     5821 actgatttag tgcacttatc gccagccact gcactacgcc ccaaagtgcc agcacccaga
     5881 agatgttcca gatgcgttta tcgagacaat ttccccacgg tacgctgtcg atatagcggc
     5941 gaatcaaata gccagagata aagaaaaaaa ccggcatacg aaagggtgca aggtaaagat
     6001 tgaaatagat ccagcatttg ctcaggactt ccgataacgg atgctgaaaa gtggtcagat
     6061 gcggataaaa ggtaatgacc gagtgataaa tcactaccag acaaatacat aaccctttga
     6121 tctggttaat ccatagctct ttttgtttca tcaggacaac acgctctttt tatttaacat
     6181 gctgttaatc ctgcggggcg agttgcgtgg tgtaatgcgt atctgtctgt atttggttat
     6241 ttttcagata aagatcagcc cacaaggccg atgagaaaat gaggcccttt agccgattga
     6301 tttacggggg cttttcagat tagccctgac gatcacttac agttcagacg tttacccatc
     6361 ccgctttcgc ttatatactc gtgtctttgc tacagcaacc agacggattt catgtaccaa
     6421 cctgtcgctc tatttattgg cctgcgttac atgcgtgggc gtgcagcgga tcgcttcggt
     6481 cgtttcgtct cctggctttc taccatcggc attaccctcg gggtgatggc gctggtcaca
     6541 gtattgtcag tgatgaacgg ctttgagcgc gagctgcaaa acaacatcct tggcctgatg
     6601 ccacaggcaa ttctctcttc tgagcatggc tctcttaacc cgcagcaact cccggaaacg
     6661 gcagtcaaac tggacggcgt taatcgcgtc gcacctatta ctaccggtga tgtggtactg
     6721 caaagcgcgc gcagcgtggc ggtcggggtg atgctgggta tcgacccggc gcaaaaagat
     6781 ccactaacgc cgtatctggt caatgtgaaa caaactgacc tcgagccggg gaaatataat
     6841 gtcatcctcg gtgaacagct tgcctcacag ttgggcgtca atcgcggtga tcaaatccgc
     6901 gtgatggtgc catctgctag ccagttcacg ccgatggggc gtattccaag tcagcgcctg
     6961 ttcaatgtga ttggcacttt cgccgccaac agtgaagtcg atggctatga aatgctggtg
     7021 aatattgagg atgcctcgcg tctgatgcgt tatccggcag gcaatattac cggctggcgt
     7081 ttgtggctgg atgagccgct gaaagtcgac tcattaagtc agcaaaaact gcctgaaggc
     7141 agcaaatggc aggactggcg tgaccgtaaa ggcgagctgt tccaggccgt acgcatggaa
     7201 aaaaatatga tgggcttgct gctgagcctg attgtcgccg ttgcggcatt taacattatt
     7261 acctcgctgg gactgatggt gatggagaag cagggcgaag tggccattct tcagactcag
     7321 gggctgactc cgcgtcaaat catgatggtc tttatggtgc aaggggccag cgccgggatt
     7381 atcggtgcga tcctcggagc ggcgcttggc gcactgcttg ccagccagtt gaataatctg
     7441 atgccgataa tcggcgtcct gcttgatggt gcggcgctgc cggtcgctat cgagccttta
     7501 caggtcattg ttattgcgct ggtggcgatg gctatcgcgc tgctgtctac gctttaccct
     7561 tcctggcgcg ctgccgccac tcaacccgct gaggctttac gttatgaata agatcctgtt
     7621 gcaatgcgac aacctgtgca aacgctatca ggaaggcagt gtgcaaaccg atgtactgca
     7681 caatgtcagt ttcagcgtgg gcgaaggtga aatgatggcg atcgtcggca gctctggttc
     7741 cggtaaaagc accttgctgc acctgttagg cgggctggat acgccaacct ccggcgatgt
     7801 gctctttaac ggtcaaccga tgagcaaact gtcgtcggcg gccaaagcgg aactgcgtaa
     7861 ccagaagctg ggatttattt atcagtttca ccacctgttg ccggatttta ccgccctgga
     7921 aaacgtggct atgccgctgt tgattggcaa gaaaaagccc gctgaaatca acagccgtgc
     7981 gctggaaatg ttaaaagcgg tagggctgga gcatcgtgcg aatcatcgcc catctgaact
     8041 ttctggcggc gaacgccagc gtgtggcgat tgcccgtgcg ctggtcaata acccgcggct
     8101 ggtactggcg gatgaaccta ccggtaacct cgatgcacgt aacgccgaca gcatcttcca
     8161 gttgcttggg gaattgaatc gcttgcaggg caccgccttc ctggtggtta ctcacgacct
     8221 gcaactggcg aaacgtatga gccgccaact ggaaatgcgt gatggacgtc tgacggcgga
     8281 actgagcctg atgggggcgg agtaatggcg atgcctttat cgttattgat tggcctgcgt
     8341 tttagccgcg gacggcgacg cggcggcatg gtgtcgttga tctccgtcat ttctaccatt
     8401 ggcattgccc tcggcgtggc ggtattgatc gtcggcttaa gcgcgatgaa cggctttgaa
     8461 cgcgaactga ataaccgcat tctggcggta gtaccgcacg gtgaaattga ggcggtagat
     8521 cagccgtgga ccaactggca ggaagcactg gataacgtgc agaaagtgcc gggtattgcc
     8581 gccgctgcgc cgtatatcaa tttcaccgga ctggtagaaa gtggtgccaa tctgcgcgca
     8641 atccaggtga agggcgttaa cccgcaacag gaacagcgtc tgagcgcatt accctcgttt
     8701 gtccagggcg atgcgtggcg caattttaaa gcgggcgaac agcaaattat catcggcaaa
     8761 ggcgtggcgg atgcgctgaa agtgaagcag ggggattggg tgtcgattat gatccccaac
     8821 tcgaatcctg agcataaact gatgcagcca aaacgtgtgc gtttgcacgt tgccggtatt
     8881 ttgcagttga gtggccaact cgatcacagt tttgccatga tcccgctggc cgatgctcag
     8941 caatatcttg atatgggttc cagcgtgtca ggtattgccc ttaaaatgac ggatgttttc
     9001 aacgccaata agctggtacg cgatgcgggg gaagtgacca acagctatgt ttatattaaa
     9061 agctggattg gtacttacgg ctatatgtat cgcgatatcc agatgatccg cgccattatg
     9121 tatctggcga tggtactggt gattggtgtg gcctgtttca acatcgtctc caccttagtg
     9181 atggcggtga aagacaagag tggcgatatc gcagtattaa gaacgctggg ggcgaaagat
     9241 ggtttaattc gcgccatctt tgtctggtat ggattgctgg cagggctgtt cggcagcctg
     9301 tgtggtgtga ttatcggcgt agttgtttca ctgcaactta ccccgattat tgagtggatt
     9361 gaaaagctga tcggtcatca gttcctctcc agcgatatct attttattga cttcttgcca
     9421 tcggaattgc actggctgga cgtcttctac gtactggtca cagcattgtt gctgagtctt
     9481 ttggcaagct ggtatccggc gcggcgcgcc agtaatattg accctgcgcg agtccttagc
     9541 ggccagtaaa ggcagtacat taaaacaagg agcggcaatg tattacgggt ttgatattgg
     9601 tggaacaaaa attgcgctag gcgtgtttga tagcggtcgg cagttgcagt gggaaaagcg
     9661 ggtgccgaca ccgcgtgaca gctatgacgc atttttagat gcagtgtgcg agctggtagc
     9721 cgaagctgac cggcgttttg gctgtaaagg ttctgtcggc atcggtattc cgggaatgcc
     9781 ggaaacagaa gatggtacgc tgtatgccgc caatgtccct gctgccagcg gtaaaccgct
     9841 gcgtgccgac ctgagcgcac gtcttgatcg cgatgtacgc cttgataacg atgccaactg
     9901 ctttgccctt tctgaagcat gggatgatga atttactcaa tatccactgg tgatggggtt
     9961 gattctcggc accggcgttg gcggcgggct gattttcaac ggtaaaccca ttaccggtaa
    10021 aagctatatt accggcgagt ttggccatat gcgtctgccg gttgatgcgt taaccatgat
    10081 ggggctggat ttcccgttac gccgctgcgg ctgtggtcag catggctgca ttgaaaatta
    10141 tctgtctggt cgcggttttg cgtggctgta tcaacactat tatcatcaac cgttgcaggc
    10201 tcctgagatc attgcgcttt atgatcaagg cgatgagcag gcaagggcgc acgttgagcg
    10261 ttatctggat ttattagcgg tttgtctggg aaatatcctg accattgttg accctgacct
    10321 ggtcgtcatt ggtggtggct tatcgaattt cccggcaatc acaacgcaac tggcggaaag
    10381 gttgcctcgt catctcttac ctgtagctcg tgttccgcgc attgaacgcg cgcgccacgg
    10441 tgatgcgggg ggaatgcgtg gtgcggcctt cctacatcta accgattaat caacagaggt
    10501 tgctatgctg tcgcgtcggg gtcatcggtt aagtcgtttt cgtaaaaata aacgccgcct
    10561 gcgcgagcgt ttgcgtcagc gtattttttt cagagataaa gtggtgccgg aagcaatgga
    10621 aaaaccaaga gtactcgtac tgacaggggc tgggatttct gcggaatcag gtattcgtac
    10681 ctttcgcgcc gcagatggcc tgtgggaaga acatcgggtt gaagatgtgg caacgccgga
    10741 aggttttgat cgcgatcctg aactggtgca aacgttttat aacgcccgtc gtcgacagtt
    10801 gcagcagcca gaaattcagc ctaacgctgc acatcttgcg ctggcgaaac tgcaagatgc
    10861 tctcggcgat cgctttttgc tggtgacgca gaatatcgac aatctgcatg aacgcgcagg
    10921 caataccaat gtgattcata tgcatgggga actgctgaaa gtatgttgtt cgcaaagtgg
    10981 ccaggtcctc gactggaccg gagacgttac gccagaagat aaatgccatt gttgccagtt
    11041 cccggcaccc ttgcgcccgc acgtagtgtg gtttggcgaa atgccactcg gcatggatga
    11101 aatttatatg gcgttgtcga tggccgatat tttcattgcc attggtactt cggggcatgt
    11161 ttatccggcg gctgggtttg ttcacgaagc gaaactgcat ggcgcgcaca ccgtggaact
    11221 gaatcttgaa ccgagtcagg ttggtaatga atttgccgag aaatattacg gtccggcaag
    11281 ccaggtggtg ccagagtttg ttgaaaagtt gctgaaggga ttataaagcg ggaaaaattg
    11341 cctgatgcgc tacgcttttc aggcctgtga aggggcgcaa ttaattgaat ttgcgggttt
    11401 tgtgggccgg ataaggcgtt cgcgccgcat ccggcataat acatgcgaac tttactgcct
    11461 cttttaccgt ttactcgtcc atagtaacgc ggcaataaac gtacagacac caatcagccc
    11521 tgcccagagt ggcgatattc tggtaatcgc aaagctgtta tcgatataca aacataaagc
    11581 ctgataaccc atattggcaa cagccatcgc cactgcaaat tttaacgcat aataaaggcg
    11641 cgttgtgtag ttaaaaaatg cagcaaagac gatgaatgca actgcccagg caaaaaggcc
    11701 taatgtcgtg gtactgccgg gtggatcatt ttcatgctgt tgtccgggat gcggataata
    11761 tgtcagtggt ggcgtcggtt gttttaatat ctgtgcttgt aaaacagcca atgcattatt
    11821 gataccctga taccagagat ccgcatgtaa ataagccagc gtgtgatttt ctaccagaag
    11881 gccgagatgt ttatcgtcca tactgctttc gacctggccg ttggttatca taattaaccg
    11941 tggctcgcgg gtagtaaaga gtaacagaac acttcctttt acattactca ttgaagatgc
    12001 cgcatgcgcc agcatcgctt tgccattttg cctgagactg gcttcattat taatgtcggg
    12061 taaaaccaca ataaacggtg caactccggt ttgttggcga aaaagttcgg ctttatgcgc
    12121 gatctcttca gcatctggta cagtaccggt aacatccgta aaaagtgtct tcattattgg
    12181 aattaagggc ttactgattg ccgtcagagg caggaacatc agtaaactga gcagtatcat
    12241 gatttttttc atgggcggga cttccgtttc ctgcgtgtct tttttgagga gggcatactg
    12301 attaataaac cgacggcgat aaaaataacg cccataatgc cgataaaccc ccatttaaaa
    12361 aaattaaccc acgggttatt tatctcagcc ttttcaggat gtcctggaga ataataaaca
    12421 ttaactttat cgccttcgct gtcttcataa aaatcgctgc caatactcga attgaaaatt
    12481 agcgtgtaat ctggcgtcgg acgaaaggca acgacgggat accacgaacc atttttaccc
    12541 gtgcgatgag aatgactgga gtgccagacg gtatcaacaa tgacgccttc agtatgaatc
    12601 gcgtctttgc tataggtgaa ctggttataa aagataacaa cagaaatgag gatcagtacc
    12661 agaccgatac cgaagaatat acgaaagaaa actctactgt cctggctcat tagccacatc
    12721 cttgctaaat aaaaaacggg cggtaatacc accgcccact tgctgaatta acgtcctgct
    12781 ttcagcttct gataatactc ttcataaatg ctgctggcgg agcctacgtc attttgccat
    12841 tcaccatttt taatggtttc agcatccggg tagagcgttt tatcgttcgc cacttccgga
    12901 cttaacagct tacgcgccgc aaggtttggc gttggataac cgatagtttc agcaacctgt
    12961 tttgccacat ccgggcgcag caggaagttg atcaatttca gcgcgccttc tttgtttttg
    13021 gcatttgccg ggatcgccag gctgtccatc cagaaaatgc cgccttcttt cggccatacc
    13081 acatcaattg gcgtacccgc ctggcgtgca acgaaagcag aaccgttcca gatcatgccg
    13141 aggttaactt cgccttccat atacgggtta gcggggttat cggagttaaa cgccgcgacg
    13201 tttggcatca gttttttcag ctcgttgtat gcagcttcaa tctctttcgg atcggtggtg
    13261 ttaccggagt agcccagctt acgcagcgcc atctggaaca cttcacgggc atcgtcggtc
    13321 agcagcaggc tgcctttgta ctcaggcttc cacagatcgg cccagctggt gacagatttc
    13381 ggatccaccg catcaccgtt aacgccaatc gccgttgcac cccagatata cggaatggaa
    13441 tagtcgttat tcgggtcaaa cggcttgttg agcatgtctg gatcgaggtt gctgaaattg
    13501 cttaacttcg acttgtcgat cttctggatc atcccttctt tacgcatttt atcgacgtaa
    13561 taggttgaag gaaccaccag gtcataggca ccgtctttgt acgttttcag cttcgcgtac
    13621 atggtttcgt tcgactcgta agtcgaatag ataaccttaa taccggtttc tttggtgaac
    13681 tgttcaagca gtcctggcgg cacgtactcg gtccagttgt agaaatacag cgtgttgttg
    13741 tcatcggcgt gagcggcgct catgcccagt gccagagcac ccgccgcgag caggtggcgt
    13801 gaccattttt tcatttaacg tcccctgtgt tacctttcgt tttatcacga gcaataagct
    13861 ggctggcaat taccatcacc agcgacagca ccagtaatat ggttgccagc gcgttaactt
    13921 ccggcgatac gccgactttg accatcgaat aaatttttaa cggcagaatt tcataactcg
    13981 gcccggtgac aaacgaagaa accaccacgt cgtccatcga cagggtaaag cttaacaccc
    14041 agcccgccgc taccgctggc attgccagag gcagaatgat ttttcgcaga atggtaaatt
    14101 cgctggcacc gagatctttc gccgcttcca gcattcgcac gtcaaaacct ttcaggcgcg
    14161 aatacactgt caccaccaca aatggcaggc agaaggtgat atgcgagaac agcagcgacc
    14221 agaagccaag ctgaataccc agcagcataa acagcaccag cagcgaaatc gccatcacga
    14281 tatctggtga catcatcacc acaaacagca ttccgctaac gaacggctta ccacgaaagc
    14341 gataacggta cagcgcaact gccgtcagtg aaccgataag cgtagcaaac gtcgccgaaa
    14401 acaccgccat tgttagtgaa tgctgcgctg cctgtaacag gctgtcgttg ttcatcagca
    14461 ggctatacca tttggtggta aaaccctgcc agttgatgcc aaagcgcgag ctgttaaagg
    14521 agttcacaat caaaataatg attgggatat acaggtacgc gtagatagcg gtcataaaac
    14581 cgccgcgaag cagtcgaccg atcattcgag ttccaccttc ttattcataa ggtggagcac
    14641 tttcaggatt ttgaatttat tcaatttttt cctttctcca aaatctctcc aaaacgtttc
    14701 cccaaaacta ggccacgcat tttacaacac ttttttcctg agatggtgac ggatattcca
    14761 tatggtgttt gtcaaataac caacttaacc cattcctgac ctcgagtatc gttatagcga
    14821 tcggtggttg cctggacttt atgtcctagt aatgtttttg tatcgatacc ctgtgcacgg
    14881 tacagccgtt ctgatagaga gcgttgttca tgaaatgttg gcggagtttt tcctgctggt
    14941 ggaattatcc cagccagatc ccgtgctttg gcaaagtagt cgctcaggtt gtctttactc
    15001 atcggcttcg gttgtttctg gtgccgacta tggattagat atggacttaa tattctgtct
    15061 cggcacccat caataacttc ttttaacgtt atcccaatgg catcacagcg tagtgtaagc
    15121 ggtaacgcca gacgcattcc ggtttttccc tgggtgatat gcaagtgttc gttccacaca
    15181 tctgaaaaac gcatgtggca aatgtcatca cggcgctgac cagtaacaat cgcaagaagc
    15241 attgcgttac ggataaagtg tttttcaggc gttgcattgt aaattttttg ccagtcttcc
    15301 atggtgagcc tggctctggt tactttaggg atcggtttac gggtagcctc cggaggattc
    15361 catccaggag gaacttcccc tgcatgctgt gcttctttat aaatatcaac ccataatcca
    15421 cgatttactc tcgctgtgct gaccatgtct ttatccagcc actcatccag tattaatgca
    15481 aagtctctta cttccagttc tttcaatggg tggtttccca gacgggaaac caggtatgca
    15541 gccattcgag ttttttcttt gtgagttgta gctgcaatat ctccattttt cagtcgcgtg
    15601 tcctgtattt tcagatatcg atcaacccat gcctttaatc tgataccccg acgttttgtt
    15661 gctgacggac tttcatcaat tttgcgcatg aaatattcag cttctgctgc agctattcgc
    15721 tgattggctg tggaagcgat tttttctgcc ttacctttgt ctgttccgag tccgtgaaat
    15781 tttccagtca caggattttt atactggtag taaactctgc cagttctgcg atcaaacttt
    15841 tcgtaaagac cggctacgtc agtgctgttt tttcgtggcc tcggtgacat gagttaaaat
    15901 ctccttcagt gcatcatcat cgccagtatg aatttccggc tcaattcccg tttcaccagg
    15961 cccaacaaat actgctcggc gatctatcag ccaacgccca cgaatttttt gtggccttgg
    16021 aacgatgtat cctagttttc cgtatttcac cagggtagtg tttgttattg ggagactgaa
    16081 ccgttttggt ttccactcgt cgagcgttat caggtactgt tcgctcatgg ctatcactcc
    16141 ggaacgcgcc agttgcagaa tatcaacgac aactggcgac ggttgaacat taaaaatcag
    16201 cctgactcgg gatcagtttt tgccagataa ctgaaacgta ttttgcctgg taacgggcgt
    16261 catcaagtgc attatggcgc tcaccttcga atggaatagc cgttctggca tcgaagtcta
    16321 tggctttccc cagctcaacg attgtgcgta catctcgatc gttgtagtaa cgccacgggc
    16381 aggggatccc ctgccgttcg tatgaacggc gcaaaatcgt gttgtcgaag ttggctccat
    16441 ttccccagac ctgaacaaaa aattcaccgg agttttcgtc gataaattcc cgcaattgta
    16501 acagtgcatc atctaacggg atttcatcgg tcataatggc agattgcgct tcgcgtgatt
    16561 gcttaagcca ccatttaatg gtgtcccgat caatgacccc gccagcagtt tccagatcga
    16621 tagtcttact aaattccggt cccatatctc cggtttgcgg atcgaaaaat attgcaccta
    16681 ttgagatgat cggggcatca ggattttttc ccatggtttc aaggtcgatc attagatggt
    16741 cacacgtcct gctggtggat gtgataacat gatgaccgtt caccgtaatt aagggatctg
    16801 ccgtctcgcc agtttcacta tcgctggcgt gatcctgagc gctgccagca ttctccttgt
    16861 gtggatgttc agcgccttcc atttccttcg gatcattttc ctgaacttca acctgattct
    16921 cttcatcgaa tgtttcctgg tatgttgcgt cgcccatcac cgcgccacaa tcagggcagt
    16981 tgccgccgcc ggtctgaccg caggcggtgc agatcttttc cggttcctgt tgcactactg
    17041 gttcaggttg tttcgtttct ggctcgtttt gtaacgcatt tgggctgttt tgttccgctt
    17101 tctggtcgtt ctgttccgat tcttgctggt tctggtttac agaatcgcgg gtttcaatcc
    17161 cctttaccca tttcggatca ttcgggtcgc taatccctgc aacaaattct ccgcgagagg
    17221 cagcaagcaa cttatcggcg tcaggctggc tgatattggc tgcctgcata attttgttta
    17281 cttcgtcagc ggtaactttt acctgtgact tgtcatccag cggctgcggg tcctgatgat
    17341 gttcaactgt atccgctgcc attgtttcag ccgttgcctg ttcatctgcc attgcgccag
    17401 atggttgcgg gttatcttca tcattttttt cttcttctgt tttgcactca gcagccagtc
    17461 cgccgttaat ttcttccagg atatcttttt ccggtgtatg ccgggcagcc atgagcgttt
    17521 cagctgtggg attctcgtga tcagtttctg tcaggttggc attgatatac ccctgaaggc
    17581 gccccgggta gtgataaaac tcagggtggg cgcttcggat aagtgcaaaa atagctgcgc
    17641 gggaatagtc caggatacca ggggttgcgc gaagtgctgc ggaccattcc ctgaacggac
    17701 tttctttttt caggacgatt tcttttgcgc gacgataaac gctgcccgga atttcataaa
    17761 tattaaaatc catcggaagt gtggctgctg caatctccac atccagcgca tcgaaggtgt
    17821 gtactaaatt cggattgcga tcggttttgt tcccgccacc ggcatttgca ccggaagccg
    17881 tacgggtgat acgcgaaaca cgatttcctt tcatccactc ttttgtcagc agaccccgat
    17941 cagtgtaatc agcgtccagg tatgcctcga aaaaagcagt tatcagtccc aggtctgaat
    18001 taccgggatt agggaaaact ttgtcagtgt cgcgtaccag tttgtgaagg tcgcgaatct
    18061 ccagcgggtc gagcagcttt gttttgtgag aaatggccag ggcagtaaca gccggcagtt
    18121 cttccgcccg tgctatatgt aatgcctgaa gttcttcccg tgcaacgtgc gttactggtt
    18181 tttcgctgct gtgttgcgca agccagcgaa tgggcagctc ctgaccagaa accggcagga
    18241 gcatattctc ctcaatctcc gtcatgtctt cgccgttgac gttggtattg tcagtactgg
    18301 ctggtttctc ctgcacggag ggagagggcg cgataaatac cattgtaatg ccatcttccc
    18361 cgcctttttc gtatcggttg cagaattcgg tatcaaacac gccttcaggt ggaaggtcat
    18421 tcacaacggg taaatggacg cgaacgggtt ttttaaagtc gtcttcatca taatcgttgt
    18481 catccattgc ggtaatgcag cgggagatgg caacagataa tttttttgct gtagtccagt
    18541 aaaaaccacc tttaattccc agacgttttc tgactttgtc attttttgct tcgcaatata
    18601 gcgcaaattc ttctttatca gtgctcattg ataaacctca ttacagattt aagggtgaac
    18661 aaatccctgc cattgctggc atttttaatc cgttggtatg gtgttaatat ggctggaggg
    18721 ttatccagcc ggtgtttcgt tattcaggta cagcgatact ttttttaccg ggaggcattc
    18781 accagaaatt ttttgctcgt ctcttgcctg gaggcaggat tctttactgg cataaattcc
    18841 ggtaatcaca ttctgtgatt cacccgttat aagaaaaacc gtcatcatca gtgcaaatgc
    18901 tgaagtcatt gacgctctcc gaaaatacca agttcaagaa gagcaattcg ggaaagtatg
    18961 gaattatcat tgagcagata aggctcatat ttcctcatat taatggcatc ttcagtaaac
    19021 tcccggttac tgagcagaac accaatatca aaacaacctt cagacgtatt aacgtttggt
    19081 aataacgttt ccattatcgc gtcctcaaca atgaattttg tgatgcagtg cctggtgcct
    19141 ccaggtgacg ttaaccagtt aacaattaac gccgggttag ttgatgctcg ttacgccagt
    19201 aaaagaccgc cttactgctt taactgttcc gcgtgcgcat agccgcattc accgcatcac
    19261 aaaattcact ttaaaaaggg cggcagagca gtcacggagt aaaactgata ccgccaaacg
    19321 tcaccagaaa attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca
    19381 acctgacaac ccggtgtcct caacggggaa ggaataaccc cgtcatactt accgccgcgc
    19441 catttcgcgg agtg
//
LOCUS       DAZFRG010000079        19381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFRG010000079 DAZFRG010000000
VERSION     DAZFRG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.079"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..1065
                     /locus_tag="ACHKUC_002163"
     CDS             136..1065
                     /locus_tag="ACHKUC_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO6618417.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            1161..3497
                     /gene="arcB"
                     /locus_tag="ACHKUC_002164"
     CDS             1161..3497
                     /gene="arcB"
                     /locus_tag="ACHKUC_002164"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO6618418.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            3727..4380
                     /gene="elbB"
                     /locus_tag="ACHKUC_002165"
     CDS             3727..4380
                     /gene="elbB"
                     /locus_tag="ACHKUC_002165"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO6618419.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            4377..5105
                     /gene="mtgA"
                     /locus_tag="ACHKUC_002166"
     CDS             4377..5105
                     /gene="mtgA"
                     /locus_tag="ACHKUC_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO6618420.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(5102..5734)
                     /gene="yrbL"
                     /locus_tag="ACHKUC_002167"
     CDS             complement(5102..5734)
                     /gene="yrbL"
                     /locus_tag="ACHKUC_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO6618421.1"
                     /translation="MIRLSEQTPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(5948..6220)
                     /gene="npr"
                     /locus_tag="ACHKUC_002168"
     CDS             complement(5948..6220)
                     /gene="npr"
                     /locus_tag="ACHKUC_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO6618422.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(6217..7071)
                     /gene="rapZ"
                     /locus_tag="ACHKUC_002169"
     CDS             complement(6217..7071)
                     /gene="rapZ"
                     /locus_tag="ACHKUC_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO6618423.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(7117..7608)
                     /gene="ptsN"
                     /locus_tag="ACHKUC_002170"
     CDS             complement(7117..7608)
                     /gene="ptsN"
                     /locus_tag="ACHKUC_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO6618424.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(7726..8013)
                     /gene="hpf"
                     /locus_tag="ACHKUC_002171"
     CDS             complement(7726..8013)
                     /gene="hpf"
                     /locus_tag="ACHKUC_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO6618425.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(8036..9469)
                     /gene="rpoN"
                     /locus_tag="ACHKUC_002172"
     CDS             complement(8036..9469)
                     /gene="rpoN"
                     /locus_tag="ACHKUC_002172"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO6618426.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(9517..10242)
                     /gene="lptB"
                     /locus_tag="ACHKUC_002173"
     CDS             complement(9517..10242)
                     /gene="lptB"
                     /locus_tag="ACHKUC_002173"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO6618427.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(10249..10806)
                     /gene="lptA"
                     /locus_tag="ACHKUC_002174"
     CDS             complement(10249..10806)
                     /gene="lptA"
                     /locus_tag="ACHKUC_002174"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO6618428.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(10775..11350)
                     /gene="lptC"
                     /locus_tag="ACHKUC_002175"
     CDS             complement(10775..11350)
                     /gene="lptC"
                     /locus_tag="ACHKUC_002175"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO6618429.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRNKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(11347..11913)
                     /gene="kdsC"
                     /locus_tag="ACHKUC_002176"
     CDS             complement(11347..11913)
                     /gene="kdsC"
                     /locus_tag="ACHKUC_002176"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO6618430.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTHIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(11934..12920)
                     /gene="kdsD"
                     /locus_tag="ACHKUC_002177"
     CDS             complement(11934..12920)
                     /gene="kdsD"
                     /locus_tag="ACHKUC_002177"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO6618431.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(12934..13911)
                     /locus_tag="ACHKUC_002178"
     CDS             complement(12934..13911)
                     /locus_tag="ACHKUC_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO6618432.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            14121..14930
                     /gene="mlaF"
                     /locus_tag="ACHKUC_002179"
     CDS             14121..14930
                     /gene="mlaF"
                     /locus_tag="ACHKUC_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO6618433.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVSRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            14938..15720
                     /gene="mlaE"
                     /locus_tag="ACHKUC_002180"
     CDS             14938..15720
                     /gene="mlaE"
                     /locus_tag="ACHKUC_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO6618434.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            15725..16276
                     /gene="mlaD"
                     /locus_tag="ACHKUC_002181"
     CDS             15725..16276
                     /gene="mlaD"
                     /locus_tag="ACHKUC_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO6618435.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            16295..16930
                     /gene="mlaC"
                     /locus_tag="ACHKUC_002182"
     CDS             16295..16930
                     /gene="mlaC"
                     /locus_tag="ACHKUC_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO6618436.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            16930..17223
                     /gene="mlaB"
                     /locus_tag="ACHKUC_002183"
     CDS             16930..17223
                     /gene="mlaB"
                     /locus_tag="ACHKUC_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO6618437.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            17383..17637
                     /gene="ibaG"
                     /locus_tag="ACHKUC_002184"
     CDS             17383..17637
                     /gene="ibaG"
                     /locus_tag="ACHKUC_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO6618438.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            17692..18951
                     /gene="murA"
                     /locus_tag="ACHKUC_002185"
     CDS             17692..18951
                     /gene="murA"
                     /locus_tag="ACHKUC_002185"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO6618439.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18999..19277)
                     /gene="sfsB"
                     /locus_tag="ACHKUC_002186"
     CDS             complement(18999..19277)
                     /gene="sfsB"
                     /locus_tag="ACHKUC_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO6618440.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
BASE COUNT         4580 a         4767 c         5190 g         4844 t
ORIGIN      
        1 gtgcatgaaa tttcggtaat tttgatttat tatgcatttg ataaaagcag gccgggacga
       61 ttgatccagg tcatcgccga attgggctaa aaatggcagg ctttggcctc ttttcaaggc
      121 gcggtctatc agattatgca gttacagaaa ttagtcaata tgtttggtgg tgatctcacc
      181 cgtcgttatg ggcaaaaggt gcataagctg acgctgcatg gcggttttag ctgccctaac
      241 cgtgacggta ccatcgggcg tggcggctgc acattctgta atgttgcctc gtttgccgat
      301 gaagcgcagc agcatcgttc cattgccgag caactggcgc accaggcgaa tttagttaac
      361 cgcgctaaac gctatctggc ctactttcag gcgtatacca gcacctttgc ggaagttcag
      421 gtgctgcgtt cgatgtatca gcaggcggtg agccaggcca atattgtcgg tttgtgtgtt
      481 ggtacccgcc cggactgcgt gccggatgcg gtgctggatc tgctttgcga atataaggac
      541 cagggctacg aagtgtggct ggagctgggg ctacaaaccg cccacgacaa aacactgcat
      601 cgcatcaatc gcggtcatga ttttgcctgc tatcagcgca cgacgcagct ggcgcgtgag
      661 cgtgggctga aggtgtgttc tcacttaatt gttggcctgc ctggcgaagg gcaggctgaa
      721 tgtttgcaaa cgctggagcg agtggttgaa accggcgtgg acggcataaa gctgcatccg
      781 ctgcatatag tgaaaggcag cattatggcg aaagcctggg aagcggggcg tttgaacgga
      841 attgaactgg aggattacac gctcactgcc ggagaaatga ttcgccatac gccaccggaa
      901 gtgatctacc accgcatttc cgccagcgcc cgtcgcccga cgctgcttgc tccgctgtgg
      961 tgcgaaaatc gatggacggg aatggtcgag ctggatcgtt atctgaatga acatggcgta
     1021 cagggatcgg cgctgggacg tccgtggcta cctccaacgg cgtaggccat tgaggggaat
     1081 tccgcatttc tcacacaatt tataacgtaa ctgtcagaat tgggtattat tggggcaggt
     1141 tgtcgagaag gaattcccta atgaagcaaa ttcgtctgct ggcgcagtat tatgttgacc
     1201 tgatgatgaa gttaggtctg gtgcgcttct caatgttgct ggcgctggcc ctcgtcgttc
     1261 ttgccattgt ggtacaaatg gcggtaacca tggtgctgca tggtcaggtc gaaagcattg
     1321 atgttattcg ttctatcttc tttggtttgc tgattacgcc gtgggcggtc tactttctat
     1381 cggtggtcgt cgagcaactg gaggagtcac gacaacgtct gtcacgactg gtgcaaaaac
     1441 tggaggagat gcgcgagcgc gatttgagcc tcaacgttca gttaaaagat aatattgccc
     1501 agctaaatca ggaaatcgcc gttcgtgaaa aagcggaagc agaactgcag gaaaccttcg
     1561 gccaactgaa aattgaaatc aaagagcgcg aagagacaca aattcagctc gagcagcaat
     1621 cctcattctt acgttccttc cttgacgctt cacccgacct ggttttttat cgtaacgaag
     1681 ataaagagtt ttccggctgt aaccgcgcga tggagctgct gaccggaaaa agcgaaaaac
     1741 aactggttca cctgaaacct gctgatgttt actcaccgga agccgctgcg aaagtcattg
     1801 aaaccgatga aaaagtgttc cgtcataatg tgtcactgac ctatgagcag tggctggatt
     1861 atcccgacgg acgcaaagcc tgctttgaaa tccgtaaagt accgtactac gaccgcgtgg
     1921 gtaaacgtca cggtttgatg ggctttggtc gcgacattac cgagcgtaag cggtatcagg
     1981 atgcgcttga gcgcgccagc cgcgacaaaa cgacgtttat ctccaccatc agtcacgaat
     2041 tgcgtacacc gctgaacggt atcgtcggcc tgagccgcat tctgctggat accgaactca
     2101 ccgccgagca ggaaaaatat ctcaaaacca tccatgtttc ggctgtcacg ttggggaata
     2161 tcttcaacga tattatcgac atggataaga tggaacggcg caaggtccag cttgataatc
     2221 agccggttga tttcaccagc ttccttgccg atctggaaaa tctctccgca ttgcaggcgc
     2281 aacaaaaagg attgcgcttt aatctggagc cgacgctgcc attaccgcat caggtcatta
     2341 ccgacgggac gcgtttacgg cagatcttgt ggaacctcat cagtaacgcc gtcaaattca
     2401 ctcagcaagg ccaggttacc gtgcgcgtgc gctacgatga aggcgatatg ctgcattttg
     2461 aagtggaaga ctctggtatc ggcattccgc aggatgagct ggataaaatc ttcgccatgt
     2521 attaccaggt gaaagacagt catggcggta aacctgccac cggcaccggt attggtctgg
     2581 ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat tacggttacc agcgaacagg
     2641 gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc ggtagcagaa gaggttgatg
     2701 atgcgtttga tgaagacgat atgcctttac cggcgctgaa tgtgctgctg gtggaagaca
     2761 ttgaactgaa cgtgattgtc gcgcgctctg tgctggaaaa gttaggtaac agcgttgatg
     2821 tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc gggcgaatac gacctggtgt
     2881 tgctggatat tcagttgcca gatatgaccg ggctggatat ctctcgtgaa ctgacgaagc
     2941 gttatccgcg cgaggattta ccgccgctgg tggccttaac cgctaacgtg ctgaaagaca
     3001 agcaagagta cctcaatgct ggaatggatg atgtgctgag taagccgctt tctgttccgg
     3061 cgctaaccgc gatgatcaag aaattctggg atacccagga tgatgaggag agtacggtga
     3121 cgacagaaga gaacagtaaa tcagaagcat tgctcgatat tcccatgctg gaacagtatc
     3181 tcgaacttgt aggaccgaag ctgatcaccg acgggttagc ggtgtttgag aggatgatgc
     3241 cgggctatgt cagcgtgctg gagtcgaatc tgacggcgca ggataaaaaa ggcattgttg
     3301 aggaagggca taaaattaaa ggtgcagcgg ggtcagtggg gttacgccat ctgcaacagc
     3361 tgggtcagca aattcagtct cctgaccttc ccgcctggga agataacgtc ggtgaatgga
     3421 ttgaagagat gaaagaagag tggcgtcacg acgtagaagt actgaaagcg tgggtggcaa
     3481 aagccactaa aaaatgaccc cggctagacc ggggtgcgcg aatactgcgc caacaccagg
     3541 gaaatcttgg ctgcgccgta aattattatg atgagttaca agggcacagc actgtttttc
     3601 aggccgcacg cagttaagat agcaaaactt aaatgatttg ttacatgaat cagttaaatg
     3661 tgtgatgcgt agcattttta ttaaaaaaat taatgtgttt cagtcaattg cgaaaggaag
     3721 atcacaatga agaaaattgg cgtaattctg agcggatgcg gcgtctatga cggttctgaa
     3781 attcatgaag cggtattgac gttgttagct atttcacgca gcggtgcgca ggcggtctgc
     3841 tttgcaccgg ataagcagca ggttgatgtt atcaaccatt taactggtga agcgatgacg
     3901 gaaacgcgca atgtgctgat tgaagcggca cgaataacgc gcggtgaaat ccgtcctctg
     3961 gcccaggccg atgccgctga actggatgcg ttgattgtgc cgggaggttt tggcgcggcg
     4021 aagaatttaa gcaattttgc cagtcttggt agcgaatgca ccgttgaccg tgaattaaag
     4081 gcgctggcac aagcgatgca tcaggccgga aaaccgcttg gttttatgtg tattgcaccg
     4141 gcgatactgc cgaaaatttt cgatttcccg ctacgtttga ccatcggtac tgatatcgat
     4201 accgcagaag tgctggaaga gatgggcgcg gagcatgtgc cgtgtcctgt cgatgatatc
     4261 gtggttgatg aagacaataa gattgtcacc accccggcat atatgctggc gcagaacatt
     4321 gcagaagcgg cgagcggcat tgataagctg gtttcccgcg tgctggttct ggctgaatga
     4381 gtaaaagccg cttaacggtg tttagtttcg ttcgccgttt tcttctgcgg ttaatggttg
     4441 tcctcgccat tttctggggc gggggcatcg cgttgtttag cgttgcgcct gttcctttct
     4501 cagcggtaat ggtcgagcgg caagtcagcg cctggctgca tggcaatttt cgttacgtgg
     4561 cacattctga ctgggtcagt atggatcaaa tctcgccgtg gatgggactg gcggtgattg
     4621 ccgcagaaga tcagaaattt cctgagcact ggggctttga tgtcgcttcc attgagcaag
     4681 ccctggcgca caacgagcgc aatgaaaacc gtattcgcgg tgcttcaacg atttctcagc
     4741 agacagccaa aaatctcttt ttatgggatg ggcgtagctg ggttcgaaaa gggctggaag
     4801 ccggattaac gctggggata gaaacagtct ggagcaaaaa gcgtatcctg acggtttacc
     4861 tgaatatcgc cgaatttggc gacggtgtgt ttggcgtcga agctgcggca caacgttatt
     4921 tccacaaacc cgcgagcaaa cttacccggt cggaagctgc attgctggca gctgtattac
     4981 ctaatccact tcgtttcaaa gtctccgcgc catcgggcta cgtgcgtagc cgtcaggcgt
     5041 ggattttacg gcagatgtat cagttaggcg gtgagccgtt tatgcaacaa caccagcttg
     5101 attaagcttc gcgcgaagtg agggccagcg ttttgctctc acgaggttgc agatctttct
     5161 gcagggcaat agccagcgcc ggttgggcga taaatcgttt ccataaccgc tcttgcttac
     5221 gcagacaaca ccacttcgac caggtcgcca gtggaatcaa tgtgctttca ccaatgttgt
     5281 cgcagacgac gggggtaacc tctgactcac tgatgcggtg acagaggata ttctgcggct
     5341 tcagcgacat cgtcacgata cggttatcct gcaaataacg cttcagctgt ttcagtaact
     5401 ggcgcagttg tgcaatgtct tcttcgtaac ggcattgttc tgcaaattcg gtcagcgtaa
     5461 tggaaggctt gccgtcgaaa tcggcaatca catcgtagac ataaccggtt ccgcaatccg
     5521 tctcaacggt accgtgatag cgcggtattc cactccagtc ttttaaccgg cgaccaagat
     5581 gcgcgtagta ctttaactca cggcggatct ctttatcgcc accatcgcca cgatggtaga
     5641 caatcttgat acagcgttgg gcatcttccg gatgcgcata acatttccga tgtcttccgg
     5701 tgcccagggg agtttgttca gataagcgaa tcatgccatc tcttggaaat attttctgac
     5761 gatctccagt atggtcgatt aacttaaacg aaatctaaac aatgcttctt aaggttttct
     5821 taaggttctt ctgaaagtaa aaaagcgcac gaaatgtaca atcgacgttg attgataacg
     5881 atttcagtac gcgcttttct tattgccggg ggagcttgaa gggatttgaa tgtcaaagtg
     5941 atgaagatta atcttcatca aaaccagagt taaagagggc gataacggcg gccagtgctt
     6001 cctcttcctg tggaccggtc gcttcaactt caatctgccg tcctttggca gaatccaaca
     6061 tcagcagcgc aataacgctg ttggcttcag cctcggtgcc ttcgtcatta cgtaacagca
     6121 cttcagcgtc aaaaccctgc attaattcaa acagcttcat tgcaggccgg gcatgcatgc
     6181 ccagcttgtt tgtgatttca acagtttgct tgacggtcat ggtttacgtt tttccagcgt
     6241 ccgatggcgt gactggacgt ttttaccgcg cgagcggaag tagtctgcca gttgctctgc
     6301 aatatacacc gaacggtgct tcccgccggt acaaccaatg gcgaccgtca ggtagctacg
     6361 gttgttggtt tccagcatag gtaaccatag ctcaagatag cttcgcgtct ggtagataaa
     6421 attgtgtact tctgtgtggc ggtcgaggaa cgcggcgaca ggtttatcaa gacctgtcat
     6481 tgggcgcagt ttcggatccc agtgcgggtt cggcaagaag cgcacgtcaa agacgtaatc
     6541 tgcatcgata gggataccgt gtttgaagcc gaaagactca aagaccatgg tcagttcacg
     6601 ttcacgttta cccagcagac gggtacgcag catttctgcc agctcgtgaa cggacatttc
     6661 tgaggtatcg acaatcagat ccgctcgcga acgcagaggc tccagcaaat cgctttcttt
     6721 gtcgatagca ctttccagcg acaggttttt gctggaaagc ggatgcagtc ggcgcgtgtc
     6781 actgtaacga cgaattaagg tattacggtc ggcatccagg aacagtagtt gcggtgagaa
     6841 agcgtcaggc aggttactca tcgcctgttc gaatatttct ggtgactccg gcatattacg
     6901 aacatcaatg ctgacggcgg cagaaatctc tcgatcggcc agagttcgag ccagatcggg
     6961 taacaacact acgggaaggt tatccacgca gtaaaaaccc atatcttcca gcgcacgcag
     7021 ggcgacagat ttacctgaac ctgaacgtcc gctgacgatc atcagtacca tgtactgttt
     7081 ctcctcacaa cgtctaaaag agacattacc gaataactac gcttcatccg gagtaccttc
     7141 ggtatccgta atgatttgat acagctcttc atcgctctgg gctgcgcgta aacggcggca
     7201 gatggttttg tccgccagac gtttcgccac cagcgacaga gtatgcaggt gcgttttagt
     7261 ttggtctgcc ggcaccagca gggcaaaaag taagtccacc ggttggttgt cgatggcatc
     7321 gaaagctata ggtgtttcga gctgaacgaa aacgccaacg gcacgcagag tatcttcttc
     7381 cagtttgcca tgcggaatgg caataccatt gccgataccg gtactgccca ttttttcccg
     7441 cgtcaggata gcttcaaaaa ccacctgagg cgccaggcta agttgtttcg ccgccagctc
     7501 gctgatgatt tccagggcgc gttttttgct ctggcagtgg acgcggcttc gcgtacattc
     7561 cctgttaaga acactgctaa gctgtagagt tgtatcatta tttgtcataa tttcacctaa
     7621 gaacctgccc actcaaacag tggaacaggc tcggagcatt aaccgtacaa atggcccgtt
     7681 gtgccgcaca acaggccgcc atgcacatgc taattgcccg gacaattagt gttgtttcag
     7741 tttatcttta tgtttggtca actggcgtgc cagcttatca attaagccat caatggcagc
     7801 gtacatgtcc tgaccttccg cgctggcatg aatttcacca ccgtttacat gcagtgttgc
     7861 atctgaggtg tgggtgacct tctccacttt cagaacaaca tagacctggt tgattcggtc
     7921 aaaatattgc tcaagtttgg caaatttggc tgtaacaaat tcgcgcagtg cctcggtgat
     7981 ctcgacgtta tttccggtaa tgttgagctg catagtgtct tccttatcgg ttgggtcaaa
     8041 cgagctgttt acgctggttt gacggcggaa tggataaaga ctctcggtac ttcgcaacag
     8101 tgcggcgtgc caccatgata ccttgttccg acagcaaaga ggttaacttg ctgtcgctca
     8161 acggtttcgc tgggttttcc gccgcgatta atttcttcac cagtgcgcga atcgctgtgg
     8221 aagaggcttc acccccgcct tcagtattga cgtgactgga aaagaaatac ttcagttcaa
     8281 aaatgcctcg tggactatgc aggtattttt gcgtggtcac gcgagatatc gtcgattcat
     8341 gcatctcgac ggcctgggcg atatcagcca gtaccatcgg tttcatatat tcttcgcctt
     8401 gctcaaagaa ggcttgctgc tgttcaacga tacagcgact cacgcgcagt agcgtatcgt
     8461 tacggctttc cagactcttg atcaaccatt tggcatcctg cagattgctg cggataaact
     8521 ggctatcacc atcgttgcgc gcgttattgc acatcgaggc gtagtgctgg ttgatttgca
     8581 aacgcggaat gctgtcactg ttgagttcta ccgtccagtg accgttatgc ttacgcacca
     8641 gcacatctgg aatgacatat tcaggttcgc cagtctggat cgactgcccg gggcgcggat
     8701 cgagcgactg gatcagattg acggcttctt tcagcacatc ttctttcaga cgcgtgacgc
     8761 gcattaaagt gcggaagtcg tgattggcta atagatcgag atgatcgcta atgatcagtc
     8821 tggcctcttc cagccatggc gtggtcttat cgaattggga gagttggatc agcaggcagt
     8881 cacgcagatc ttttgccgcc acaccgaccg gatcaaacct ttggatccgc ttaaggacgg
     8941 cttcaacttc gtcgatgtca atctcttcat cgcccatact ttcgagaata tcttccagcg
     9001 ggacagtcag ataaccggtg tcatcaacgg catcgacgat agaggtagca atagcgcggt
     9061 cagtgtcgga aaacggtgtc aactcgacct gccacatcag gtaatcctgt aaggtctgtg
     9121 tcgtttcgcc ctgatagacc ggcagctcgt cgtcaatgta gtcaccgctg gtgccggatg
     9181 gtgtaccagc ggtgtaaatg gtgtcccaac tggcatcgag cggcagctct tccggcatct
     9241 ctttttgttc gagcgcgtcg gcggtgtcca gtgtttcact gtcttgcgtt tcgcgggtgt
     9301 cgatttcttc atgagtatcg atttgctcaa gcagcggatt actctccagc gcctgctgta
     9361 gctcctgctg aagttccagc gtcgacaact gcaacagacg aattgcctgt tggagctgtg
     9421 gcgtcatcgc cagttgttgg ctaagcctga gttgcaaacc ttgcttcatg ttcagaatcg
     9481 tactctcctg ctaaaacgtc gcaaacttct accctatcag agtctgaagt cttccccaag
     9541 gtatacacgc ttaacgtgtt cgtcttgtaa gatttctgta ggcgtgccgt gggcgatcaa
     9601 atgcccctga ctgacgatat aagcgcgttc acaaaccgcc agtgtttcac gcacgttgtg
     9661 gtcagtgatc agtacgccca ggccgctgtc gcgcaggtgc tcaatgatgc gtttaatgtc
     9721 gataaccgag atcgggtcaa ccccggcaaa cggttcgtcg agcagaataa atttcggatt
     9781 cgcagccagt gcgcgggcaa tttctacacg gcgacgttca cccccggaga gtgactgccc
     9841 catgctgtca cgcaggtgct caatgtgaaa ctcttccatc aactcgttcg cgcggtcttc
     9901 acgttgttca gcagacaagt catcacgaat ttgcagtacc gccatcaggt tatcgtaaac
     9961 gctgaggcga cggaaaatgg aggcttcctg cggcagatag ccgataccgc ggcgcgcgcg
    10021 tgcatgcaga ggcagcagac tgatatcgtc atcatcaata atgatgttgc ccgcatcgcg
    10081 cggcacaatg ccaacaacca tgtagaaagt ggtggtctta ccggcaccgt ttggccccag
    10141 cagaccgaca atttccccgg agttgacggt caggctgacg tcttctacca cgcgacggcc
    10201 tttataggct tttgcaaggt tctttgcagt taatgttgcc ataacgaatt aattaccctt
    10261 cttctgtgcc ggggtctggc ctttattgtt tttgtcctgc agctgcgacg gcaccagaac
    10321 ggtggttacg cgcttgcctt tgtcgctgaa agcctgcatt ttctgctctt tcaccaggta
    10381 agtgatctta tcgcccttaa tgttgctatc gacctgctgc agataagcat tacccgtcag
    10441 aacgacaaaa tcttttgcca gttcgtagtg catctgggaa gcgtgacctt caacgggttt
    10501 accgttgtcc tgcatctggt agaacgttgc cggtttaccg tagccgtcaa tcacttcttt
    10561 accttgttcg ccgcccggac gggtaacgac cactttgtcg gcattaattt tgatggtgcc
    10621 ctgggtgacg atgacattgc cggtaaaggt aaccacgttg ccttgcatat caagagattg
    10681 ctggtccgat tcaatgtgga tcggctgatc agtgtctccg gttacggcaa atgccggaat
    10741 gctggcggcc agaagtgagc tggcaagcac aagattaagg ctgagtttgt ttgttttgaa
    10801 tttcatagga tgttctaacc ttttcaatca gctcggcgtt cttgttgcgt aagttgccgc
    10861 gcattttcag accgctggag ttaaatgttg ttccgtataa cgtgacgagg tcttcagagg
    10921 taacatcctg cgtcaccaga ttgatctgcg cgttatccgt cgtgattctg cgaagttgag
    10981 agtccggcac gagtgcattg acttcaacgt gtccatataa atagagcatc cggtcattgg
    11041 tcagcttggc tttatctgct tttacggacc atgtcgggat tttatcctta tcaaacgtgg
    11101 taagtaccgg ctgcgtaaac cacgaaacgg cctgatcgga ataatattca acgtgttgag
    11161 caatcaatcg atagcttagt gccccttctg ggttatagac gagcgtgtcc gtatgctcgc
    11221 ttttataggt gggatcattg ttgttgacga ccacctgggc ggtatcgtct ttttcggcca
    11281 tattaatgcc gatcatcacc agaaccgcca gtgatagcac aatgataacc caacgtctgg
    11341 ctttactcat atcgattgcc ctttggcttc atccagtttg ccctgcgcca ggagtaataa
    11401 gtcgcaaact tctcgcactg cgccacgacc gccagcaatg tgcgttacgt aatcggcgcg
    11461 cgggatcaac agtggatgcg catcggccac ggcgacgctt aaacccactt tttccattac
    11521 cggccagtcg atgagatcat cgccgacata agccacattt tccggggcaa tcgccagttt
    11581 ttccagcaga tcgctaaagg cgatcagttt gtttgactgc ccctgataca agtgagtgat
    11641 ccccaatgtg gcacaacgat cttctaccag tttagccttt cgcccggtaa tgatagcgac
    11701 ttcaatatca gaggtgagcg cacaacgaat gccataaccg tcacgaacat tgaacgcttt
    11761 cagctcttcg ccgttattgc ccatataaat caggccatct gacagtacgc catcaacgtc
    11821 gaggatcagc agacgaatgt tctctgcttt tgccataacg tcggcgctga cagggccgta
    11881 acaggtcgca agcgacgcac ctgctttgct cattgttgtt tatccttgaa tctttacact
    11941 acgcctgcac gcagtaaatc atgcatatgt aacacaccga gtaaatggtc gccatcggca
    12001 accatcacgg aggtgatatg gcgggactgc attaagttca gtgcctcaac ggccagaatg
    12061 cctgggcgca cgcgtattcc ccccggcgtc atcacatcgg caatacttaa ctgacgaaca
    12121 tccacgccca tatcgaagac acggcgtaaa tcaccgtcgg taaagatgcc ttcaatcatc
    12181 atattgtcat cgcaaatgac agtcatacca agatttttgc gggtaacttc cagcaatgcg
    12241 tcacgcagac tggccgtttt cttaacatgc gggatctcat cgcccgtatg cataatatcg
    12301 tttacgcgca gcagaagttt acgacccagt gcgccgcctg ggtgtgagag cgcaaaatct
    12361 tcagcagtaa agccgcgtgc ttttaacagc gcgacagcga gggcatcgcc cataaccagc
    12421 gtggcggttg tgctgctggt cggtgccagc cctaacggac aggcttcttt cgccacttta
    12481 acacacagat gcacatctgc ggcgcgcgcc atgctgctct ccgggcgacc ggtgatgcag
    12541 attaacggta cgtgaagacg cttaagcact ggaattaagg ccgtgatttc gctggattca
    12601 cctgagttag agatagcaat caccacatcc tgtggggtga ccatgcctaa atcaccatgc
    12661 gcggcttcgc caggatggac gaaaaatgaa ggtgtaccgg tgctggcaaa tgttgctgcc
    12721 attttgcgcc cgatgtgccc cgatttgccc atccccatga cgacaacttt ccctttacac
    12781 cagaacatct tttcacaggc aagcgtgaaa ttctgattga tgtattgatc aagctccgcc
    12841 aggcattcac gttcaatcgc caggacttct ttacctgctt gctgaaagtc aaaacccggt
    12901 tgtaactcta cgtgcgacat aatgcgtttc cagttattca acgagtattg gcgataacca
    12961 gtacaacatc gccagccata cgataaatcc gccagttaat aataccccta caccacggcc
    13021 cggttgcggg gagcgccgcc agcacagcaa cgcaaaaata atgctcacca gcaacatcac
    13081 gctgtagtca cgactgtacg ccagtggatc aatctctcct ggcgttatca gcgcgggtaa
    13141 acccaacacg atgacaatat taaaaatgtt tgcgccaatg atatttccga ccgcaatgtc
    13201 gttttcacct ttgcgaaccc ccgctattgc ggttgccagt tccggcaggc tggttccgat
    13261 agcaattgcc gtcagaccca tcgtcaactc gctgatggca aagtaattcg ccagcaccgt
    13321 ggcgttatca actaccatcc gcgtggccac tggcataatg atcagtgcga tgccgagcca
    13381 taaaaatgcg acgggcaatc caccttcacg cggcagctct gcaagctgct ctctggtcag
    13441 gctgtcagtc ccctgacgtt cagcctgacg tgcaagttta acaatgaaca gcagccatag
    13501 cacagccaga aagaggagaa agataccatc gctgcgacta agttgtccgt catagagtac
    13561 ggaaccggcc accacgctga ccaacaacat taagggtaat tcacggcgta gaacatcaga
    13621 atggacggta aaaggacgaa ccagcgcagc cagaccgagg atcagcaata tattgataat
    13681 gtttgagccg agggctgtac caacggctaa atcgcgttgt tcgtgcaaag acgcggcaag
    13741 cgagacgatg atttctggta acgatgtacc aatactgacc accgtcatgc cgatgatcaa
    13801 cggcgggatg ccaaaggttc ggcaaagaat agacgcggca aaaaccaggc ggtcggcact
    13861 gtaaacgacc aaaagtaaac caacaattaa cagtgccgta gctaaaagca tctaacgtcc
    13921 tttcttcagg tatactcgcc ggtccgctga agattttcag aaagccgtaa cagattctta
    13981 attttgactt tatgcggcta aaaagtaaaa caaatgccag ctttcgctaa ccacggcagg
    14041 taatattctg taaatatgtt gggttcaagg ttaaattgag cgccatgctt agaaaatcaa
    14101 cgcaagacga agggtgaatt atggagcagt ctgtggcgaa tttagtcgat atgcgcgatg
    14161 tcagttttac gcgtggcaat cgctgcatct tcgataatat ttccctgacc gtgtcgcgag
    14221 ggaagatcac ggcgatcatg gggccatcgg gcatcggtaa aacgacgcta ctccgtctga
    14281 ttggcgggca aatcgcacca gatcatggtg agatcctttt cgatggtgag aatattccgg
    14341 cgatgtctcg ttcgcgcctg tatacagtgc gcaaacggat gagcatgtta tttcagtccg
    14401 gggcgttgtt cactgatatg aacgtatttg acaacgtcgc ctatccactg cgggaacata
    14461 cccaacttcc cgcgccattg ttgcatagta cggtgatgat gaagctggag gccgtggggc
    14521 tgcgtggagc ggctaaactt atgccttctg aactttccgg tgggatggcg cggcgtgcag
    14581 cgctggcacg tgcgattgcg ctggagccgg atctcatcat gtttgatgag ccctttgttg
    14641 ggcaagatcc cattaccatg ggcgtgctgg tgaagctgat ttctgagctg aacagcgcgc
    14701 tgggcgtgac ttgtgtggtg gtttctcacg atgtgccgga agtgttaagt attgcggatc
    14761 acgcctggat cctggcggac aaaaaaattg tcgctcatgg cagtgcccag gcgttgcagg
    14821 cgaatcctga tccgcgcgta cgtcagtttc tggacgggat agctgacggg cctgttccgt
    14881 tccgctatcc tgccggcgat tatcacgctg atcttttacc agggagttaa gccactcatg
    14941 ctgttaaatg cgctggcgtc gctcggacat aaagggatta aaaccctgag aacgttcggg
    15001 cgggccgggt taatgttatt caatgcgctg gtcggcaaac cggaatttcg caaacatgca
    15061 ccgctgctgg tgcgccagct ctataatgtc ggcgtcctgt cgatgctgat tattgttgtt
    15121 tctggcgtgt tcatcggaat ggtgttgggg ctgcaaggtt atctggttct gaccacttat
    15181 agtgcggaaa ccagtctggg tatgctggtg gcgttatcgc tactgcgtga actggggccg
    15241 gtggttgccg cgttgttgtt tgccgggcgt gctggttcgg cgctaaccgc agaaatcggc
    15301 ctgatgcgcg ctacagagca actctctagt atggagatga tggcggtgga tccgctgcgt
    15361 cgggttattt ctccccgttt ctgggctgga gttatttcat taccactgtt gacggttatc
    15421 ttcgtcgccg tggggatctg gggcggatcg ctggtcggcg tcagttggaa aggcattgat
    15481 agtgggttct tctggtcggc aatgcaaaat gccgtcgact ggcgtatgga tctcgtcaac
    15541 tgtctgatta agagcgtggt gttcgccatc acggtgacgt ggatttcgtt gtttaacggc
    15601 tacgacgcca tcccgacgtc tgccgggatt agccgggcaa ccactcgcac cgttgtccac
    15661 tcgtctctgg ctgttctggg gctggatttt gtgctgaccg cattgatgtt tgggaattga
    15721 gttcatgcaa acgaaaaaaa atgaaatttg ggtgggtatc tttttattag cagcactgct
    15781 tgcggcgctg tttgtttgcc tgaaggcagc gaacgtgacg tctatacgta ctgaaccgac
    15841 ctatacgctt tatgcgacgt tcgataacat tggcggcctg aaagcccgct ctccggtcag
    15901 cattggtggc gttgttgtgg gccgggtggc ggatattacg ctggacccga aaacctatct
    15961 gccgcgcgta acgctggaaa ttgaacaacg ttataaccac attcctgata ccagttcgct
    16021 gagcattcgt acttccggcc tgctggggga acaatatctg gcattaaacg tcggttttga
    16081 agacccggaa ctgggaactg ctatcctgaa ggatggcgat acaattcagg acaccaagtc
    16141 tgcgatggtg ctggaagatc tcattggtca gttcctttac ggtagtaaag gcgatgacaa
    16201 taagaatagt ggcgatgcgc cagctgctgc gccaggtaat aatgaaacca ctgaacctgt
    16261 gggtacaacg aaataatttc aggagaaccg acgcatgttt aaacgtttaa tgatggtcgc
    16321 tttgctggtg attgcaccgc tgagtgcggc aaccgcggca gaccagacca atccgtataa
    16381 gctgatggac gaggcggcgc agaaaacgtt cgatcgcctg aagaatgagc aaccgcaaat
    16441 tcgggccaac ccggattatc tgcgtaccat tgttgatcag gaactgttgc catacgtaca
    16501 ggtgaaatac gccggtgcgc tggtgctggg ccagtattac aagagtgcga ccccagctca
    16561 acgtgatgct tactttgccg ctttccgtga gtacctgaag caggcttacg gtcaggcgct
    16621 ggcgatgtat cacggtcaaa cctatcagat tgcgccagaa cagccgctgg gtgataaaac
    16681 cattgtgccg attcgcgtta ccattattga cccgaatggc cgcccgccgg tgcgtctgga
    16741 cttccagtgg cgtaaaaact cccagacggg caactggcag gcttacgaca tgattgctga
    16801 aggcgtcagt atgatcacca ccaaacaaaa cgagtgggga acgctgctgc gtaccaaagg
    16861 tatcgacggc ctgactgcgc aactgaaatc gatttctcaa cagaaaatca ctctggaaga
    16921 gaaaaaataa tgagcgagtc actgagctgg atgcagacgg gtgacacgct ggcgttatcc
    16981 ggagagctgg atcaggacgt tctgctaccg ctttgggaaa tacgtgagga agcggtgaag
    17041 gggattacca gcatcgatct tagccgtgtg tctcgcgtgg atacgggtgg actggcactg
    17101 ctgctccatc ttatcgatct ggcgaaaaag cagggcaaca atgtgacgct tcagggggta
    17161 aacgacaaag tgtataccct ggcgaaattg tataatttgc ctgctgatgt tctgcctcgt
    17221 taaatttttt caggccggat atggcgttaa ccgcctgtcc ggcaaacctg cctgcaagtc
    17281 gagccttgtc tgatttatca gcgaggcttt ttgcttattt atgccagcgc aactttgctc
    17341 taagatattt cgctgattta ttaactgatg attgaagatc ccatggaaaa taatgaaatt
    17401 cagagcgtgt tgatgaacgc tctctccctc caggaagtcc acgtttccgg cgatggcagc
    17461 cactttcagg ttattgccgt gggtgagttg tttgacggca tgagtcgggt taaaaaacag
    17521 cagacggtct atggcccgct gatggaatat attgcggata accgcattca tgctgtgtcg
    17581 atcaaagcgt atacccctgc ggagtgggcg cgcgatcgca aactgaacgg cttttgagct
    17641 atgggcgatt cgccggtagc ggatatgaat tgttaactga gaacaaacta aatggataaa
    17701 tttcgtgttc aggggccaac gaagctccag ggcgaagtca caatttccgg cgctaaaaat
    17761 gctgctctgc ctatcctttt tgccgcacta ctggcggaag aaccggtaga gatccagaac
    17821 gtcccgaaac taaaagacgt cgatacatca atgaagctgc taagccagct gggtgcgaaa
    17881 gtagaacgta atggttctgt gcatattgat gcccgcgacg ttaatgtatt ctgcgcacct
    17941 tacgatctgg ttaaaaccat gcgtgcttct atctgggcgc tggggccgct ggtagcgcgc
    18001 tttggtcagg ggcaagtttc actgcctggc ggttgtacga tcggcgcacg tccggttgat
    18061 ctacacattt ctggcctcga acaattaggc gcgaccatca aactggaaga aggttacgtt
    18121 aaagcttccg tcgatggtcg tttgaaaggc gcacatatcg tgatggataa agtcagcgtt
    18181 ggcgcaacgg tgaccatcat gtgtgctgca acccttgcgg aaggcaccac gattattgaa
    18241 aacgcagcgc gtgaaccgga aatcgtcgat accgcgaact tcctgattac gctgggtgcg
    18301 aaaattagcg gtcagggcac cgatcgtatc gtcatcgaag gtgtggaacg tttaggcggc
    18361 ggtgtctatc gcgtgctgcc ggatcgtatc gaaaccggta ctttcctggt ggcggcggcg
    18421 atctctcgcg gcaaaattat ctgccgtaac gcgcagccag atactctgga cgccgtgctg
    18481 gcgaaactgc gtgacgctgg agcggacatc gaagtcggcg aagactggat tagcctggat
    18541 atgcatggca aacgtccgaa ggctgttaac gtacgtaccg cgccgcatcc ggcattccct
    18601 accgatatgc aggcccagtt cacgctgttg aatctggtgg cagaagggac cggattcatc
    18661 accgaaacgg tctttgaaaa ccgctttatg catgtgccag agctgagccg tatgggcgcg
    18721 cacgccgaaa tcgaaagcaa taccgttatt tgtcacggtg ttgaaaaact ttctggcgca
    18781 caggttatgg caaccgatct gcgtgcatca gcaagcctgg tgctggctgg ctgtattgcg
    18841 gaagggacga cggtggttga tcgtatttat cacatcgatc gtggctacga acgcattgaa
    18901 gacaaactac gcgctttagg tgcaaatatt gagcgtgtga aaggcgagta attgtctgag
    18961 agctggcagc cccgttcgcg gggctaccgc caggccactc attttttcgg tttagtgtag
    19021 ctacgcatca actgcgttct gtcgataaac tcatgggtct gcggatcatg gtagcgtgat
    19081 ggccagataa cccaggggtc agttcccagg gctttcgcaa taatcatctc tcctttcggc
    19141 catgggcgtg ataatgcgtt cgccagcgtt gaggaactca aaccatttct gcgagactcc
    19201 gccgccattg acgttccctt cttgcgcaaa cctgcaatga tgtcagcggg atgccagtca
    19261 atgaaattac tttccatctt tcctcactcc ttaagttagt cgctttcatt cagaacactt
    19321 cttctgtttc agtcgagcat cactatactc ctgatggcct attgctctaa aaagttcctg
    19381 t
//
LOCUS       DAZFRG010000080        19227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFRG010000080 DAZFRG010000000
VERSION     DAZFRG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.080"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            307..1446
                     /gene="wza"
                     /locus_tag="ACHKUC_002187"
     CDS             307..1446
                     /gene="wza"
                     /locus_tag="ACHKUC_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO6618441.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1452..1895
                     /gene="wzb"
                     /locus_tag="ACHKUC_002188"
     CDS             1452..1895
                     /gene="wzb"
                     /locus_tag="ACHKUC_002188"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO6618442.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1898..4060
                     /gene="wzc"
                     /locus_tag="ACHKUC_002189"
     CDS             1898..4060
                     /gene="wzc"
                     /locus_tag="ACHKUC_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO6618443.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4189..5028
                     /gene="wcaA"
                     /locus_tag="ACHKUC_002190"
     CDS             4189..5028
                     /gene="wcaA"
                     /locus_tag="ACHKUC_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO6618444.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTKLKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5031..5519
                     /gene="wcaB"
                     /locus_tag="ACHKUC_002191"
     CDS             5031..5519
                     /gene="wcaB"
                     /locus_tag="ACHKUC_002191"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO6618445.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYDVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5516..6733
                     /gene="wcaC"
                     /locus_tag="ACHKUC_002192"
     CDS             5516..6733
                     /gene="wcaC"
                     /locus_tag="ACHKUC_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO6618446.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQDKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6708..7925
                     /gene="wcaD"
                     /locus_tag="ACHKUC_002193"
     CDS             6708..7925
                     /gene="wcaD"
                     /locus_tag="ACHKUC_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO6618447.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMITLGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7936..8682
                     /gene="wcaE"
                     /locus_tag="ACHKUC_002194"
     CDS             7936..8682
                     /gene="wcaE"
                     /locus_tag="ACHKUC_002194"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO6618448.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8698..9246
                     /gene="wcaF"
                     /locus_tag="ACHKUC_002195"
     CDS             8698..9246
                     /gene="wcaF"
                     /locus_tag="ACHKUC_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO6618449.1"
                     /translation="MQDLSGFSVPKGFRGSNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9273..10394
                     /gene="gmd"
                     /locus_tag="ACHKUC_002196"
     CDS             9273..10394
                     /gene="gmd"
                     /locus_tag="ACHKUC_002196"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6618450.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10397..11362
                     /gene="fcl"
                     /locus_tag="ACHKUC_002197"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10397..11362
                     /gene="fcl"
                     /locus_tag="ACHKUC_002197"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO6618451.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11365..11844
                     /gene="gmm"
                     /locus_tag="ACHKUC_002198"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11365..11844
                     /gene="gmm"
                     /locus_tag="ACHKUC_002198"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6618452.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEERAGVPGL"
     gene            11841..13055
                     /gene="wcaI"
                     /locus_tag="ACHKUC_002199"
     CDS             11841..13055
                     /gene="wcaI"
                     /locus_tag="ACHKUC_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO6618453.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAASSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKVAQLA
                     TAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVDAL
                     RNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMA
                     QQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVI
                     TAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQAFLLPKHNTVAREYAERTLDKE
                     NVLRQFINDIRG"
     gene            13058..14494
                     /gene="cpsB"
                     /locus_tag="ACHKUC_002200"
     CDS             13058..14494
                     /gene="cpsB"
                     /locus_tag="ACHKUC_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6618454.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGERYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14687..16057
                     /gene="cpsG"
                     /locus_tag="ACHKUC_002201"
                     /gene_synonym="manB"
     CDS             14687..16057
                     /gene="cpsG"
                     /locus_tag="ACHKUC_002201"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6618455.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLCDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16112..17506
                     /gene="wcaJ"
                     /locus_tag="ACHKUC_002202"
     CDS             16112..17506
                     /gene="wcaJ"
                     /locus_tag="ACHKUC_002202"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO6618456.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17508..18986
                     /gene="wzxC"
                     /locus_tag="ACHKUC_002203"
     CDS             17508..18986
                     /gene="wzxC"
                     /locus_tag="ACHKUC_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO6618457.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISYLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSSALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4604 a         4805 c         5346 g         4472 t
ORIGIN      
        1 tgtcacctta tattcggaaa ttaatttccg caataaccct ttctcatcaa cgactgtatg
       61 cagagagggt ttgaaaaatt aagctaaatc agtgtattgg tagctaaaaa gccaggggcg
      121 gtagcgtgtc tggatgcctg agagaccagt ctgaattatt ctgtcaatag cctgcggatg
      181 aaatcaattt ttttaggact gatgcctgtt aaatttttat tcaattaact gcaaggtaaa
      241 tgtgcatggc gacattgcta ttaatagtgc acaggataat tactctgcca aagtgataaa
      301 taaacaatga tgaaatccaa aatgaaattg atgccattat tggtgtcagt aaccttgata
      361 agcggttgca cagtacttcc gggcagcaat atgtcgacga tgggtaaaga cgtcatcaaa
      421 cagcaggacg ctgatttcga tctcgacaaa atggtgaatg tttatccgct gaccccgcgc
      481 ctgattgacc aattacgccc acgccccaac gtagcgcgcc ccaatatgac gctggaaagc
      541 gagatcgcga attaccagta tcgcgtcggg ccgggggacg ttcttaatgt caccgtctgg
      601 gatcacccgg agctcaccac gccagccggt cagtaccgca gctccagcga caccggcaac
      661 tgggtacagc ctgacggcac catgttctac ccgtatatcg gcaaggttca cgtcgtgggg
      721 aaaacgctcg ctgaaatccg cagtgatatt accgggcgct tagcgacgta catcgctgat
      781 ccgcaggtgg acgttaatat cgccgccttc cgctcgcaaa aagcctatat ctccggtcag
      841 gtgaataaat ccggtcaaca ggccattacc aacgtgcctc tgaccattct cgacgctatc
      901 aacgccgcag gtggcctgac cgacaccgct gactggcgca acgtggtgct aacacacaat
      961 ggtcgtgaag agcgcatttc tttgcaggcg ctgatgcaaa acggcgacct caatcagaac
     1021 cgactgcttt accccggcga tattctttac gtgccacgta atgatgatct gaaagtgttt
     1081 gtgatgggtg aagtgaagaa acagagcacc ctgaaaatgg actttagcgg catgaccctg
     1141 actgaagccc taggcaatgc tgaaggtatc gacatgacca cctccaacgc cagcggcatc
     1201 tttgtcattc gtccgctgaa aggcgagagc gggcgtaacg gcaagattgc caatatctac
     1261 cagctggata tgtccgatgc gacgtcgctg gtaatggcga cagaattccg cctgcaacct
     1321 tatgacgtgg tgtatgtcac caccgccccg gtatcccgct ggaaccgtct gatcaatcag
     1381 ttgctgccaa ctattagcgg tgtccgttac atgacggata cagccagcga cattcataac
     1441 tggtaatcgt catgtttaac aacatcttag ttgtctgtgt cggcaatatt tgccgttccc
     1501 cgacggcgga acgcttactg caacgttatc acccggagct aaaagtggag tccgctggac
     1561 tcggcgcgct ggtcggaaag ggcgctgatc ctaccgctat cagcgtcgcc gcagaacatc
     1621 aactgtctct ggaaggtcat tgcgcacggc aaatcagccg ccgtctgtgt cgcaactacg
     1681 acctgattct gaccatggaa aagcgccata tcgaacgctt atgcgagatg gcacccgaga
     1741 tgcgcggcaa agtgatgctg tttggtcact gggataacga atgtgaaatc cccgatccat
     1801 atcgcaaaag ccgggaaacg tttgcagcgg tgtacacatt acttgaacgg tctgcccgcc
     1861 agtgggcgca ggcattgaac gcagagcagg tataagaatg acagaaaaag taaaacaaca
     1921 tgccgctccg gtaacgggca gtgatgaaat cgatattggt cgcctggtcg gcaccatcat
     1981 tgaagcacgc tggtgggtga ttggcattac cgccgtattc gccctctgtg ccgtggttta
     2041 caccttcttc gctacgccga tatacagcgc cgacgcactg gtacaaatcg aacaaagcag
     2101 cggcaattcg ttagtgcagg atatcggatc ggcattagcc aacaaaccgc ctgcatcgga
     2161 cgccgagatc cagttgattc gttcgcgtct ggtgcttggt aaaacggtgg acgatctcga
     2221 ccttgatatt gcggtgagca aaaacacgtt cccgattttc ggtgcgggct gggatcgcct
     2281 gatgggacgc cagaacgaga cggtgaaagt gaccaccttt aaccgaccga aggagatggc
     2341 ggatcaggtg tttacgctta atgtgctgga caacaaaaac tacaccctga gcagcgatgg
     2401 cggctttagc gcccgtgggc aagcgggcca gatgctgaaa aaagaaggcg tcacgctgat
     2461 ggttgaagcc attcacgcca gcccgggcag tgagtttacc gtcaccaaat actccacgct
     2521 ggggatgatc aaccaactgc aaaacagcct gacggtaacg gagaacggca aagacgcagg
     2581 tgttctgagc ctgacttaca ccggtgaaga tcgcgaacag atccgcgaca ttcttaacag
     2641 catcgcccgt aactatcagg aacaaaatat tgagcgcaaa tcggcggaag cgtcgaaaag
     2701 cctcgctttc ctcgcgcaac agttaccgga agtacgtagc cgccttgatg ttgccgaaaa
     2761 caaactgaat gccttccgtc aggataaaga ttcagttgat ctgccgctgg aagcgaaagc
     2821 ggtgctcgat tcgatggtga acatcgacgc gcagttgaac gaactgacct ttaaagaggc
     2881 ggaaatctcc aagctgtaca ccaaagttca cccggcgtac cgcacgctgc tggagaaacg
     2941 gcaggcgctg gaagacgaaa aagccaaact taatggtcgc gtaacggcga tgccgaaaac
     3001 ccagcaggaa attgtgcgtc tgacccgcga tgtcgagtct ggtcagcagg tctatatgca
     3061 actgctgaat aaagagcagg agctgaaaat caccgaagcc agcaccgtcg gcgatgtgcg
     3121 cattgtcgac ccggcaatca cccagccagg tgtactgaaa ccgaagaaag ggctgattat
     3181 ccttggggcg attatccttg gcctgatgct ctctatcgtg ggtgtgctgc tgcgctcgtt
     3241 gtttaatcgc ggtatcgaaa gcccgcaggt gctggaagaa cacggtatca gcgtttatgc
     3301 cagcatcccg ctgtcggagt ggcagaaagc gcgcgatagc gtcaaaacca tcaaagggat
     3361 taaacgctat aaacagagcc agctactggc ggtggggaat ccaaccgatc tggcgattga
     3421 agccatccgt agtctgcgta ccagtctgca cttcgcgatg atgcaggcac agaacaatgt
     3481 gttgatgatg accggggtta gcccgtcaat cggtaaaacc tttgtctgcg ccaacctggc
     3541 ggcggtgatt agccagacca ataaacgggt gttgttgatc gactgcgata tgcgcaaagg
     3601 ctacacccac gagctgttgg gcactaataa cgttaatggc ctgtcggaaa ttctgattgg
     3661 acagggcgat attactaccg ctgctaaacc gacctctatt gccaaatttg acctgatccc
     3721 gcgcggtcag gtaccgccaa atccttctga actgttaatg agcgaacgct ttgccgaact
     3781 ggtgaagtgg gcgagtaaaa actacgacct ggtgttgatt gatacgccgc cgattctggc
     3841 agtgaccgat gcggcaattg ttggtcgtca tgtcggaacc acgttgatgg tggcgcgtta
     3901 tgcggtcaac acattgaaag aagtggaaac cagtctgagc cgctttgagc aaaacggtat
     3961 tccggtgaaa ggggtgattc tgaactccat cttccgccgc gccagcgcgt atcaggatta
     4021 tggctattac gaatacgaat ataagtcgga tgcgaaataa cgagatccgc tgtcggatgc
     4081 gatgctgacg catcttatcc gacctacgtt cacgccgcat ccggcaacag aacctgtaaa
     4141 ccagcttata aaccggcaga acatctcgat actccgggag aacaacctat gaaagacaat
     4201 ccgctgattt caatctatat gccgacctgg aaccgccaac aactggcgat tcgggcgata
     4261 aaatcggtcc tgcgccagga ctacaacaac tgggagatga tcatcgtgga tgattgctcc
     4321 acctcctggg aacaactaca acagtacgtc accgccctca acgatccgcg tatcacttac
     4381 attcacaacg acattaacag cggggcgtgc gcggtacgta accaggcgat tatgctggcg
     4441 caaggggaat acatcaccgg gatcgatgac gacgatgaat ggatacccaa ccgcctgagc
     4501 gtcttcctcg cccataaaca gcaactggtc acacacgcct ttttgtacgc taacgactac
     4561 gtttgccagg gcgaagttta ttcccaaccg gcaagcttgc cgctgtatcc caaatcgccg
     4621 tattcacgcc gcctgtttta caaacgcaat atcattggca atcaggtctt tacctgggcg
     4681 tggcgtttca aagagtgctt gttcgatacc aaactgaaag cggcacagga ttacgacatc
     4741 ttcctgcgga tggtggtgga gtacggcgaa ccgtggaaag tggaagaggc gacgcagatc
     4801 ctgcatatca atcatggtga aatgcagatc acctcgtcac cgaaaaaatt ctccggatac
     4861 ttccactttt accgcaagca caaagacaaa ttcgaccgcg ccagcaaaaa atatcagctg
     4921 tttaccctct accagatccg caataaacgc atgacctggc gcactttgct gacgctgctg
     4981 tcggtgcgta acggcaaacg tctggctgat ggtattcggg ggcgctaaag atgctggaag
     5041 atctgcgcgc caacagctgg agtttacgtc cgtgctgcat ggttcttgcc tatcgcgtcg
     5101 ctcatttttg ttcggtgtgg cgcaaaaaga acgtcctcaa caatctgtgg gcggccccgc
     5161 tgctggtgct gtatcgcatt atcaccgaat gctttttcgg ttatgaaatc caggccgccg
     5221 cgaccatagg tcgacgcttt actatccatc acggttacga cgtagtgatc aataaaaacg
     5281 tggtcgcggg ggatgatttc accattcgcc acggcgtcac tatcggcaat cgtggtgccg
     5341 ataacatggc atgtccacac attggcaacg gcgtcgaact cggtgccaac gtcattattc
     5401 ttggtgatat cacgcttggt aacaacgtca ccgtgggcgc gggcagcgtg gtgctcgact
     5461 ctgtcccgga caacgcgctg gtggtgggag aaaaagcgcg agtgaaggta attaaatgaa
     5521 tattctgcaa ttcaatgtgc gactggcgga aggcggggca gcaggtgtgg cgttagatct
     5581 ccaccagcgt gcgctgcaac aggggctggc atcacatttt gtctacggct acggcaaagg
     5641 cggcaaagag agcgtcagtc atcaaaacta tccgcaggtc atcaaacata cgccgcggat
     5701 gaccgcgatg gcgaacattg cgttgtttcg tctgtttaat cgcgatctgt tcggcaattt
     5761 caatgagtta tatcgcacca ttactcgcac accgggtccg gtggtcctgc attttcatgt
     5821 gctgcacagc tactggttga atcttaagag cgtggtgcgc ttttgcgaaa aagtgaaaaa
     5881 ccataaaccg gatatcactc tggtctggac gttacacgat cactggagcg ttaccggacg
     5941 ctgcgccttt accgacggtt gcgaaggctg gaaaacgggc tgccagaaat gcccgacctt
     6001 aaataactat ccaccggtga agattgatcg cgcgcaccag ctggtggcgg gtaaacgcca
     6061 gttattccgc gagatgctgg cactgggctg tcagtttatt tcccccagcc agcatgtggc
     6121 tgatgctttc aatagtctgt acggtccagg gcgttgccgg attatcaata acggtattga
     6181 tatggcaacc gaagcgattc tggcggactt gcctccggtg cgcgaaaccc aggacaagcc
     6241 gaaaatcgcg gtggtggcgc atgatctgcg ttacgacggc aaaactaacc agcaactggt
     6301 acgtgagatg atggcgctgg gcgacaaaat tgaactgcat acctttggta agttctcgcc
     6361 gttcaccgct ggcaacgtgg ttaatcacgg ctttgaaact gacaagcgca agttgatgag
     6421 cgcgctcaat cagatggatg cgctggtatt cagttctcgc gtcgataact acccgctgat
     6481 tttgtgtgag gcgctatcga ttggcgtgcc ggtgattgcc acccatagcg acgcggcgcg
     6541 ggaagtgctg caaaaatccg gcggtaaaac cgtcagcgaa gaagacgtgc tgcaactggt
     6601 gcagttaagc aaaccggaaa tcgcgcaggc gatatttggt accacgctgg ctgagttcag
     6661 ccaacgcagc cgcgccgcct acagtggaca acagatgctg gaggagtatg tcaacttcta
     6721 tcagaatctg tagctacctg ctgctgccgc tgatttacct gttggttaat gtcaaaatcg
     6781 cccagcttgg cgaaagtttc cccatcacca tcgtcacttt tttacctgtc ttgttactgc
     6841 tgtttttaga acgcatcagc gttaaaaaat tgatgattac cttagggatt ggcgcgggac
     6901 tcacggcgtt taactacctg tttggtcagt cgttggatgc cggtaaatac gtcacttcaa
     6961 ccatgctgtt tgtctatatt gtgatcatta ttggcatggt gtggagtatt cgctttaaaa
     7021 cgatttcgcc acacaaccat cgcaagatat tacgtttctt ttatctggtg gtcgggctgg
     7081 tggtggcgct ggcggcagtg gagatggcgc aaattatcct caccggcggc agcagtatta
     7141 tggagtcgat ttcgaaatat ctgatttaca gcaacagcta tgtgctgaat ttcatcaaat
     7201 tcggcggcaa gcgcacgaca gcactttatt tcgaaccggc atttttcgct ctggcattaa
     7261 tctcaatttg gctcagcatc aaacagtttg gtatcaaaac gcctaaaacc gatgctatga
     7321 ttctcgcagg gataatatta tccggatcgt tttcaggggt tatgaccttt atcctgtttt
     7381 atttgctgga gtgggcattt caatatctga ataaagaggc gattaagaaa aagttaccgt
     7441 tagcattgat ttctctggct gtattcctgg ttggtgtggt aatcgcgttt ccttatattt
     7501 ccacccgtct gggcgattta ggtacagaag gatcgtcatc gtattatcgt attgtcggtc
     7561 cgctggtgat ggtcggttat tctttgaccc atattgacgg tgtagtcaga tttggctcac
     7621 tttatgaata tgtcgcatca ttcggaatat ttaacggtgc ggatgtcgga aaaaccatag
     7681 acaatggttt gtatctgctg attatttatt tttcctggtt cgcagtgttt ttatcgctgt
     7741 ggtacatggg gaaagtgata aaaatgatga tcaacgcttt tggtgataac cgcaattttc
     7801 gcgtgcaatt atatcttttt actccggtat cgctgttttt taccggttcg atatttagcc
     7861 cggaatatgc atttttaatc gtctgtccgt ttattttgcg aaaagcgtta aatattacga
     7921 ggtaagaata agaacatgtt gcttagcata atcactgtcg cgtttcgtaa cctcgcaggg
     7981 atagtcaaaa cacatgcctc gctggcgcat ctggcgcagg cggacgatat cagcttcgaa
     8041 tggattgttg ttgatggcgg ctccaacgac ggcacccgtg agtatctgga aaatctcaat
     8101 ggtatctata acctacgctt tgtcagcgaa ccagataacg gtatctacga cgccatgaac
     8161 aaaggtattg aaatggcgca aggcaagttc gcgctgtttc tcaattcagg cgatattttt
     8221 catcaggatg ccgcaaattt tgtccgtaag ttaaaagtgc aaaaagataa catgatgatc
     8281 accggcgatg cgctgctgga ttttggcgac gggcataaaa ttaaacgtag cgccaaaccg
     8341 ggttggtata tttaccacag cctgcccgcc agtcatcagg cgatattttt cccggtgtcc
     8401 ggcttgaaga aatggcgtta tgacctggaa tataaagttt cttccgatta cgcgctggcg
     8461 gccaaaatgt ataaagccgg ttatgcattt aaaaaactca atggcctggt gtctgaattt
     8521 tctatgggtg gggtatctac caccaataat atggaattgt gtgctgacgc caaaaaagtc
     8581 caacggcaga tattgcacgt gcctggcttt tgggctgaat tatcctggca tttacgccaa
     8641 cgtacgacct caaagacgaa agccttatat aacaaaagct gaatataagg gaaaacgatg
     8701 caggatttaa gcggtttctc ggtgccgaaa gggttccggg gcagcaatgc tattaaagtg
     8761 caattgtggt gggcggtaca ggcaacaata tttgcctggt cgccacaagt attgtatcgc
     8821 tggcgggcat ttttattacg tttattcggc gcaaaaatag gaaaaaacgt agttattcgt
     8881 ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag gtgattacgc gtgggtcggc
     8941 gatgaggtca atttatatac cctcggtgaa ataaccattg gcgcacattc ggtgatatcg
     9001 caaaaaagtt atttatgcac cgggagccac gaccatgcaa gtcaacattt caccattaac
     9061 gccacgccta ttgtgattgg cgagaaatgc tggctggcaa ccgatgtctt tgttgcccca
     9121 ggcgtcacga tcggcaacgg caccgtcgtg ggtgcgcgaa gcagtgtttt taaatcgctt
     9181 ccggcaaatg tggtttgccg ggggaacccc gcagtggtga tacgcaaacg cgttgaaact
     9241 gaataaattc aaaaaataca gaggaataag acatgtcaaa agtcgctctc atcaccggtg
     9301 taaccggaca agacggttct tacctggcag agtttctgct ggaaaaaggt tacgaggtgc
     9361 atggtattaa gcgtcgtgca tcgtcattca acaccgagcg cgtggatcac atttatcagg
     9421 atccgcacac ctgcaacccg aaattccatc tgcattatgg cgacctgagt gatacctcca
     9481 acctgacgcg cattttgcgt gaagtgcagc cggatgaagt gtacaacctg ggcgcaatga
     9541 gccacgttgc ggtttctttt gagtcaccgg aatataccgc agacgttgat gcgatgggga
     9601 cgctgcgcct gctcgaggcg atccgcttcc tcggtctgga aaagaaaacc cgtttctatc
     9661 aggcttctac ttctgaactg tacggtctgg tgcaggaaat tccgcagaaa gagaccacgc
     9721 cgttctaccc acgctctccg tatgcggtcg ccaaactgta cgcctactgg atcaccgtta
     9781 actaccgcga atcctacggc atgtacgcct gcaacggtat tctctttaac catgaatccc
     9841 cgcgccgtgg cgaaaccttc gttacccgca aaatcacccg cgcaatcgcc aacatcgccc
     9901 aggggctgga atcgtgcctg tacctcggca atatggattc cctgcgtgac tggggccatg
     9961 ccaaagacta cgtaaaaatg cagtggatga tgctgcaaca ggaacagcca gaagatttcg
    10021 ttatcgcgac cggcgttcag tactccgtac gtcagttcgt ggaaatggcg gcagcacagt
    10081 tgggcatcaa actgcgcttt gaaggcacgg gtgttgaaga gaagggcatt gtggtttccg
    10141 tcaccgggca tgacgcgccg ggcgttaaac cgggcgatgt gattatcgcc gttgacccgc
    10201 gttacttccg tccggcggaa gttgaaacgc tgctcggcga cccgaccaaa gcgcacgaaa
    10261 aactgggctg gaaaccggaa atcaccctca gagagatggt gtctgaaatg gtggctaatg
    10321 acctcgaagc ggcgaaaaaa cactctctgc tgaaatctca cggctacgac gtggcgatcg
    10381 cgctggagtc ataagcatga gtaaacaacg catttttatc gctggccatc gcggaatggt
    10441 cggttccgcc atcaggcgac agctcgaaca gcgcggtgat gtggagctgg tattacgcac
    10501 ccgcgatgaa ctgaacctgc tggacagccg cgcggtgcat gatttctttg ccagcgaacg
    10561 tattgaccag gtctatctgg cggcggcgaa agtgggcggc attgttgcca acaacaccta
    10621 tccggcggat ttcatctacc agaacatgat gattgagagc aacatcattc acgccgcgca
    10681 tcagaacgac gtgaacaaac tgctgtttct cggatcgtcc tgtatctacc cgaaactggc
    10741 aaaacagccg atggcagaaa gcgagttgtt gcagggtacg ctggagccga ccaacgaacc
    10801 ttacgccatt gccaaaatcg ccgggatcaa actgtgcgaa tcttacaacc gccagtatgg
    10861 acgcgattac cgctcggtca tgccgaccaa cctgtatggc ccgcatgaca acttccaccc
    10921 gagtaattcg catgtgatcc ctgcattgct gcgtcgcttc cacgaggcga cggcacagaa
    10981 tgcgccggac gtggtggtat ggggcagcgg tacaccgatg cgtgaatttc tacacgttga
    11041 tgatatggca gcggcgagca ttcatgtcat ggagctggcg cacgaagtct ggctggaaaa
    11101 cacccaaccg atgctgtcgc acattaacgt cggcacgggc gttgactgca ctatccgcga
    11161 gctggcgcaa accatcgcca aagtggtggg ttacaaaggt cgggtggttt ttgatgccag
    11221 taaaccggat ggtacgccgc gcaaactgct ggatgtgacg cgcctgcatc agcttggctg
    11281 gtatcacgaa atctcactgg aagcggggct tgccagcact taccagtggt tccttgagaa
    11341 tcaagaccgc tttcgggggt aatgatgttt ttacgtcagg aagactttgc cacggtagtg
    11401 cgctccacac cgcttgtctc tctcgacttt attgtcgaga acagtcgcgg cgagtttctg
    11461 cttggcaaaa gaaccaaccg cccggcgcag ggttactggt ttgtgccggg agggcgcgtg
    11521 cagaaagacg aaacgctgga agccgcattt gagcggctga cgatggcgga acttgggttg
    11581 cgtctgccga taacagcagg ccagttttac ggtgtctggc agcactttta tgacgataac
    11641 ttctccggca cggatttcac tactcactat gtggtgctcg gttttcgctt cagagtagcg
    11701 gaagaagagc tgttactgcc ggatgagcag catgacgatt accgctggct gacgccggac
    11761 gcgttgctcg ccagtgacaa tgttcacgct aacagccgcg cctattttct cgctgaggag
    11821 cgtgccggag tacccggatt atgaaaatac tggtctacgg cattaactac tcgccggagt
    11881 taaccggcat cggcaaatac accggcgaga tggtggaatg gctggcagca caaggtcatg
    11941 aggtgcgggt cattaccgcg ccgccttact acccgcagtg gcaggtgggc gagaactatt
    12001 ccgcctggcg gtacaaacgg gaagaggggg ccgccacggt gtggcgctgc ccgctgtacg
    12061 tgccaaaaca gccgagcacc ctgaaacgcc tgttgcatct cggcagtttt gccgccagca
    12121 gtttctttcc gctgatggcg caacgtcgct ggaagccgga tcgcattatc ggcgtggtgc
    12181 caacgctgtt ttgcacgccg ggaatgcggc tgctggcgaa actgtctggt gcgcgcacgg
    12241 tgctccatat tcaggattac gaagtggacg ccatgctggg gctgggcctt gccggaaaag
    12301 gcaaagtggc acagctggca acggcgttcg aacgtagcgg actgcataac gtcgataacg
    12361 tctccacgat ttcgcgctca atgatgaata aagctatcga aaaaggcgtg gcggcggaaa
    12421 acgtcatctt cttccccaac tggtcagaaa ttgcccgttt tcagcatgtc gcagacgccg
    12481 atgttgatgc ccttcgtaac cagcttggcc tgccggataa caaaaaaatc attctttact
    12541 ccggcaatat tggtgaaaag caggggctgg aaaacgttat tgaagctgcc gatcgcctgc
    12601 gcgatgaacc gcttattttt gccattgtcg ggcagggcgg cggcaaagcg cggctggaaa
    12661 aaatggcgca gcagcgtgga ctgcgcaaca tgcaattttt tccgctgcaa tcgtatgacg
    12721 ctttacccgc attgctgaag atgggggatt gccatctggt ggtgcaaaaa cgcggcgcgg
    12781 cagatgccgt attgccgtcg aaactgacca atattctggc ggtaggcggt aacgcggtga
    12841 ttaccgcaga agcccacaca gaactggggc agctttgcga aacctttccg ggcattgcgg
    12901 tttgcgtgga accggaatcg gtcgaggcgc tggtggcggg gattcgtcag gcgttcctgc
    12961 tgcccaaaca caacacggtg gcacgtgaat atgccgaacg cacgctcgat aaagagaacg
    13021 tgttacgtca atttataaat gatattcggg gataattatg gcgcagtcga aactctatcc
    13081 agttgtgatg gcaggtggct ccggtagccg cttatggccg ctttcccgcg tactttaccc
    13141 caagcagttt ttatgcctga aaggcgatct caccatgctg caaaccacca tctgccgcct
    13201 gaacggcgtg gagtgcgaaa gcccggtggt gatttgcaat gagcagcacc gctttattgt
    13261 cgcggaacag ctgcgtcaac tgaacaaact caccgagaac attattctcg aaccggcagg
    13321 gcgtaacacc gcacctgcca ttgcgctggc ggcgctggcg gcaaaacgtc atagcccgga
    13381 gaacgaccca ttaatgctgg tgctggcggc ggatcatgtg attgccgacg aagatgcgtt
    13441 ccgcgccgcc gtgcgtaacg ccatgccgta tgctgaagcg ggcaagctgg tgaccttcgg
    13501 cattgtgccg gatctacctg aaaccggata tggctatatt cgtcgcggtg aagtgtctgc
    13561 gggtgagcag gatacggtgg cctttgaagt ggcgcagttt gtcgaaaaac cgaatctgga
    13621 aaccgctcag gcctatgtgg caagcggcga atattactgg aacagcggca tgttcctgtt
    13681 ccgtgccgga cgctatctcg aagaactgaa aaaatatcgc ccggatattc tcgatgcctg
    13741 cgaaaaagcg atgagcgccg tcgatccgga tctcgatttt attcgtgtgg atgaagaggc
    13801 gtttctcgcc tgtccggaag agtcggtgga ttacgcggtc atggaacgca cggcagatgc
    13861 cgttgtggtg ccgatggatg cgggctggag cgatgtcggt tcctggtctt cattatggga
    13921 gatcagcgcc cacaccgccg agggcaacgt ttgccatggc gatgtgatta atcacaaaac
    13981 tgaaaacagc tatgtgtacg ccgaatctgg cctggtcacc accgtcgggg tgaaagattt
    14041 ggtggtagtg cagaccaaag atgcagtgct gattgccgac cgtaatgcgg tgcaggatgt
    14101 gaaaaaagtg gtcgagcaga tcaaagctga tggtcgccat gagcatcggg tgcatcgcga
    14161 agtgtatcgt ccgtggggca aatatgactc tatcgacgcg ggcgagcgct accaggtgaa
    14221 acgcatcacc gtgaaaccgg gcgaaggctt gtcggtacag atgcatcatc atcgcgcgga
    14281 acactgggtg gttgtcgcgg gaacggcaaa agtcactatc gacggtgata tcaaactgct
    14341 tggtgaaaac gagtccattt atattccgct gggggcgacg cattgcctgg aaaacccggg
    14401 gaaaattccg ctcgatttaa ttgaagtgcg ctccggctct tacctcgaag aggatgatgt
    14461 ggtgcgcttc gcggatcgct acggacgggt gtaaacgtcg catcaggcgc tgtttgtcgg
    14521 atgcggtgtg aacgccttat ccgacctacg gttcgggttt gtaggcctga taagacgcgg
    14581 cagcgtcgca tcaggcaatg aatgcgaaac cgcgatgtaa ataacgacaa aaacaaaatt
    14641 ggccgtttcg gtcagggcca actattgcct gaaaaagggt aacgatatga aaaaattaac
    14701 ctgctttaaa gcctatgata ttcgcggaaa attaggcgaa gaactgaatg aagatattgc
    14761 ctggcgcatt ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg
    14821 tgatgtccgc ctcaccagcg aaaccttaaa actggcgctg gcgaaaggtt tacaggatgc
    14881 gggcgtcgac gtgctggata ttggtatgtc cggcaccgaa gaaatctatt tcgccacgtt
    14941 ccatctcggc gtggatggcg gcatcgaagt taccgccagc cataacccga tggattacaa
    15001 cggcatgaag ctggtgcgcg aaggggctcg cccgatcagc ggcgataccg gactgcgcga
    15061 cgtccagcgt ctggcagaag ccaacgactt tcctcccgtc gatgaaacca aacgcggtcg
    15121 ctatcagcaa atcaatctgt gtgacgctta cgttgatcac ctgttcggtt atatcaacgt
    15181 caaaaacctt acgccgctca agctggtgat taactccggg aacggcgcgg cgggtccggt
    15241 ggtggacgcc attgaagccc gctttaaagc cctcggcgca ccggtggaat tgatcaaagt
    15301 gcataacacg ccggacggca atttccccaa cggtattcct aacccgttgc tgccggaatg
    15361 tcgcgacgat acccgcaatg cggtcattaa acacggcgcg gatatgggca ttgcctttga
    15421 tggcgatttt gaccgctgtt tcctgtttga cgaaaaaggg cagtttattg agggctacta
    15481 cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca
    15541 cgatccacgt ctctcctgga acaccgttga tgtggtgact gccgcaggcg gcaccccggt
    15601 aatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag acgccatcta
    15661 cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg acagcggcat
    15721 gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaact
    15781 ggtgcgcgac cgtatggcgg cttttccggc aagcggtgag atcaacagca aactggcaca
    15841 acctgttgag gcgattaacc gcgtggaaca gcactttagc cgcgaggcgc tggcggtgga
    15901 tcgtaccgat ggcatcagca tgacctttgc cgactggcgc tttaatctgc gctcctccaa
    15961 caccgaaccg gtggtgcggt tgaatgtgga atcacgcggt gatgtgccgc tgatggaagc
    16021 gcgaacgcga actctgctga cgttgctgaa cgagtaatgt cggatcttcc cttaccctac
    16081 tgcgggtaag gggctaaaaa caggaacaac gatgacaaat ctaaaaaagc gcgagcgagc
    16141 gaaaaccaat gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt
    16201 tgccgggcta tggctggttt gcgaagtgag cggactgtca ttcctctaca tgcacctgtt
    16261 ggtggcgctg attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg
    16321 ctcatggcgc ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacgtt
    16381 aagcgtgatt ttcagcgccg gactggtggc gttcaacaat gatttcgaca cgcaattgaa
    16441 aatctggctg gcgtggtatg ggctgaccag catcggactg gtggtttgcc gttcatgtat
    16501 tcgcattggg gcgggctggc tgcgtaatca tggctataac aagcggatgg tcgcggtggc
    16561 gggggattta gccgccggac aaatgctgat ggagagcttc cgtaatcagc cgtggttagg
    16621 gtttgaagtg gtgggcgtat accacgaccc aaaaccgggc ggcgtttcta acgactgggc
    16681 gggcaatctg caacagctgg tagaggatgc aaaagcgggc aagattcata acgtctatat
    16741 cgcgatgcag atgtgcgacg gcgcgcgagt gaaaaaactg gtccatcaac tggcggacac
    16801 cacctgttcg gtgctgctga tccccgatgt ctttaccttc aacattctcc attcccgtat
    16861 cgaagagatg aacggcgtac cggtggtgcc gctttacgac acgccgcttt ccggggttaa
    16921 ccgcctgctc aaacgtgcgg aagatattgt gctggcaacg ctgatcctgc tgctgatctc
    16981 cccggtgctg tgctgtattg cgctggcggt aaaactcagt tcaccagggc cggttatttt
    17041 tcgccagact cgctacggca tggatggcaa gccgatcaaa gtgtggaagt tccgttccat
    17101 gaaagtgatg gagaacgaca aagtggtgac ccaggcgacg cagaacgatc cgcgcgtcac
    17161 caaagtgggg aactttctgc gccgcacctc gctggacgaa ttgccgcagt ttatcaatgt
    17221 gctgaccggg gggatgtcga ttgtcggtcc acgtccgcac gcggtggcgc ataacgaaca
    17281 gtatcgccag ctcattgaag gctacatgct gcgccataag gtgaaaccgg gcattaccgg
    17341 ctgggcgcag attaacggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg
    17401 cgtcgagttc gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt
    17461 tttcctgacg gtattcaagg gcttcgttaa caaagcggca tattgatatg agcttacgtg
    17521 aaaaaaccat cagcggcgcg aagtggtcgg cgattgccac ggtgatcatc atcggcctcg
    17581 gtctggtgca aatgactgtg ctggcgcgga ttatcgacaa ccaccagttc ggcctgctga
    17641 ctgtgtcgct ggtgatcatc gcgttggcag atacactttc tgacttcggt atcgctaact
    17701 ccattattca gcgaaaagaa atcagctacc ttgaactcac cacgctgtac tggctgaacg
    17761 tcgggctggg gcttgtggtg tgcgtggcgg tgtttttgtt gagtgatctc atcggcgact
    17821 tgctgaataa cccggacctg gcaccgttga ttaaaacatt gtcgctggcg tttgtggtga
    17881 tcccccacgg gcaacagttc cgcgcgttga tgcaaaagga gctggagttc aacaaaatcg
    17941 gcatgatcga aaccagcgcg gtgctggcgg gctttacttt tacggtggtt agcgcccatt
    18001 tttggccgct ggcgatgacc gcgattctcg gttatctggt caatagtgcg gtgagaacgc
    18061 tgctgtttgg ctactttggc cgcaaaattt atcgccccgg tctgcatttc tcgctggcgt
    18121 cggtggcacc gaacttacgc tttggtgcct ggctgacggc ggacagcatc atcaactatc
    18181 tcaacaccaa cctttcaacg ctggtgctgg cgcgtattct cggcgctggc gtggctgggg
    18241 gatacaacct ggcgtacaac gtggccgttg tgccaccgat gaagctgaac ccgatcatca
    18301 cccgcgtgtt gtttccggcg ttcgccaaaa ttcaggacga tacagaaaag ctgcgcgtca
    18361 acttctacaa gctgctgtcg gtagtgggga ttatcaactt tccggcgctg cttgggctga
    18421 tggtggtgtc gaataacttt gtaccgctgg tctttggtga gaagtggaac agcattattc
    18481 cggtgctgca attgctgtgt gtggtggggc tgctgcgctc cgtgggtaac ccgattggtt
    18541 cgctgctgat ggcgaaagcg cgggtcgata tcagctttaa attcaacgta ttcaaaacct
    18601 ttctgtttat tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc
    18661 ttggcttcct gctggtgcag attatcaaca ccattctgag ctatttcgtg atgattaaac
    18721 cggtgctcgg ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttatctct
    18781 cgctgccgac gctggtggtt agttctgcgc tgggcattgt gctgaaaggg caactggcgc
    18841 tggggatgct gctggcggtg caaatagccg cgggcgtgct ggcgtttgtg gtgatgattg
    18901 tgctgtcgcg ccatccgctg gtggtggaag tgaagcgtca gttttgtcgc agcgaaaaaa
    18961 tgaaaatgct tttacgggcg gggtgaatgg ttgttttcgg ttccggcaac ggttgccgga
    19021 tgcggcgtaa aacgccttat ccgtcctggg gggctgggct tggatcttgt aggcctgata
    19081 agacgcgcca gcgtcgcatc aggcactgaa tgccggatgc ggcgtgaacg ccttatccgg
    19141 cctacggttc tgtgctcgga tcttgtaggc ctgataagac gcgtcagcgt cgcatcaggc
    19201 actgaatgcc ggatgcggcg taaaacg
//
LOCUS       DAZFRG010000081        19083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFRG010000081 DAZFRG010000000
VERSION     DAZFRG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.081"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1246
                     /gene="pdeC"
                     /locus_tag="ACHKUC_002204"
     CDS             <1..1246
                     /gene="pdeC"
                     /locus_tag="ACHKUC_002204"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO6618458.1"
                     /translation="FLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTY
                     MQRGNYVAVINPLFWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDL
                     TVQRNGYLYATVYSTKRPIAAIVATSYQRLITHFYNYLIFALPAGILGSLVLLLLWLR
                     IRQNYLSPKRKLQRALEKNQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEF
                     IPLAEKEGMIGQVTDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQK
                     TEQHAVRPQQIKFEVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSL
                     NVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGV
                     RYCQGWFFAKAMPPQVFMQWMG"
     gene            complement(1282..1605)
                     /gene="soxS"
                     /locus_tag="ACHKUC_002205"
     CDS             complement(1282..1605)
                     /gene="soxS"
                     /locus_tag="ACHKUC_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO6618459.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1691..2155
                     /gene="soxR"
                     /locus_tag="ACHKUC_002206"
     CDS             1691..2155
                     /gene="soxR"
                     /locus_tag="ACHKUC_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO6618460.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2701..4050
                     /gene="ghxP"
                     /locus_tag="ACHKUC_002207"
                     /gene_synonym="yjcD"
     CDS             2701..4050
                     /gene="ghxP"
                     /locus_tag="ACHKUC_002207"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO6618461.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4201..5850
                     /gene="yjcE"
                     /locus_tag="ACHKUC_002208"
     CDS             4201..5850
                     /gene="yjcE"
                     /locus_tag="ACHKUC_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6618462.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5886..6770)
                     /locus_tag="ACHKUC_002209"
     CDS             complement(5886..6770)
                     /locus_tag="ACHKUC_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6618463.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            6874..7284
                     /locus_tag="ACHKUC_002210"
     CDS             6874..7284
                     /locus_tag="ACHKUC_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6618464.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            7277..7966
                     /locus_tag="ACHKUC_002211"
     CDS             7277..7966
                     /locus_tag="ACHKUC_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFO6618465.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDMLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARVMF"
     gene            complement(8005..9654)
                     /gene="actP"
                     /locus_tag="ACHKUC_002212"
     CDS             complement(8005..9654)
                     /gene="actP"
                     /locus_tag="ACHKUC_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO6618466.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9651..9965)
                     /gene="yjcH"
                     /locus_tag="ACHKUC_002213"
     CDS             complement(9651..9965)
                     /gene="yjcH"
                     /locus_tag="ACHKUC_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO6618467.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(10348..12306)
                     /gene="acs"
                     /locus_tag="ACHKUC_002214"
     CDS             complement(10348..12306)
                     /gene="acs"
                     /locus_tag="ACHKUC_002214"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO6618468.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGHPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12698..14134
                     /gene="nrfA"
                     /locus_tag="ACHKUC_002215"
     CDS             12698..14134
                     /gene="nrfA"
                     /locus_tag="ACHKUC_002215"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO6618469.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            14179..14745
                     /gene="nrfB"
                     /locus_tag="ACHKUC_002216"
     CDS             14179..14745
                     /gene="nrfB"
                     /locus_tag="ACHKUC_002216"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO6618470.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14742..15413
                     /gene="nrfC"
                     /locus_tag="ACHKUC_002217"
     CDS             14742..15413
                     /gene="nrfC"
                     /locus_tag="ACHKUC_002217"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO6618471.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            15410..16366
                     /gene="nrfD"
                     /locus_tag="ACHKUC_002218"
     CDS             15410..16366
                     /gene="nrfD"
                     /locus_tag="ACHKUC_002218"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO6618472.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            16386..18104
                     /gene="nrfE"
                     /locus_tag="ACHKUC_002219"
     CDS             16386..18104
                     /gene="nrfE"
                     /locus_tag="ACHKUC_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO6618473.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSVLFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGALLSGWR
                     GRKRDE"
     gene            18097..18480
                     /gene="nrfF"
                     /locus_tag="ACHKUC_002220"
     CDS             18097..18480
                     /gene="nrfF"
                     /locus_tag="ACHKUC_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO6618474.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            18477..19073
                     /gene="nrfG"
                     /locus_tag="ACHKUC_002221"
     CDS             18477..19073
                     /gene="nrfG"
                     /locus_tag="ACHKUC_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO6618475.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSKQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
BASE COUNT         4167 a         4859 c         5300 g         4757 t
ORIGIN      
        1 ttttttatgc tcatcgctga tagcgtctgt aaatggctat acgattgcac cggccgatta
       61 taagcgcgaa cctaacgttt ctatctatta ttaccgtgat acgccttttt tctctggcta
      121 taaaatgacc tacatgcagc ggggaaatta tgtggcggtt atcaaccctc tcttctggag
      181 tgaagtgatg tctgatgatc cgacattgca atggggagtg tatgatacgg tgacgaaaac
      241 ctttttctcg ttaagcaacg aggcctcggc agcaacgttt tctccgctga ttcatttgaa
      301 tgatttaacc gtacaacgaa atggctattt atatgcgaca gtttattcga caaaacgccc
      361 aattgcagcc attgttgcga cttcatatca acgtcttata actcattttt ataattatct
      421 tatttttgcg ttgcccgccg gtattttggg gagtcttgtt ctgctattac tctggctacg
      481 tattcgacaa aactatttgt ctcccaaacg caaattgcaa cgcgccctcg aaaaaaatca
      541 actttgcctt tattaccagc caataatcga tatcaaaaca gaaaaatgta tcggcgctga
      601 agcgttgtta cgttggcctg gtgagcaggg gcaagtaatg aatccggcag agtttattcc
      661 gctggcagaa aaggagggga tgatcggaca ggtaactgat tatgttattg ataatgtctt
      721 ccgtgatttg ggcgcgtatc tggcaacaca taccgatcgc tatgtttcta ttaacctgtc
      781 agcctccgat tttcatacct cacggttgat agcgcgaacc aatcagaaaa cagagcaaca
      841 tgcggtgcgc ccgcagcaaa ttaaatttga agtgactgaa catgcatttc ttgatgtcga
      901 caaaatgacg ccgattattc tggctttccg caaggccggt tacgaagtgg caattgatga
      961 ttttggtatt ggctactcta acttgcataa ccttaaatca ttgaatgtcg atattttgaa
     1021 aatcgataaa tcgtttgttg agacgctgac cacccacaaa accagtcatt tgattgcgga
     1081 acacatcatc gagctggcgc acagcctggg gttaaaaacg atcgctgaag gtgtcgaaac
     1141 tgaggcgcag gttaactggc tgcgcaaacg cggcgtgcgc tattgccagg gatggttttt
     1201 tgcgaaggcg atgccgccgc aggtgtttat gcaatggatg gggtaattac ccgcgcggga
     1261 gttaacgcgc gggcaataaa actacaggcg gtggcgataa tcgctgggag tgcgatcaaa
     1321 ctgccgacgg aaaacgcggg agaaggtctg ctgagagaca taacccaggt ccattgcgat
     1381 atcaaaaatc ggacgctcgg tggtgcgcaa ctcaacggcg gccagtaaca gacggcgctg
     1441 gcgaatgtaa tcgccaagcg tctgatgcgt caccgtgcgg aacatccgtt gcaagtacca
     1501 ctttgaatag ccagattttt ttgcgacgac atcaatgtta agcggctggt caatatgctc
     1561 gtcaatccat gcgataagat cctgaataat tttctgatgg gacataaatc tgcctctttt
     1621 cagtgttcag ttcgttaatt catctgttgg ggagtataat tcctcaagtt aacttgaggt
     1681 aaagcgattt atggaaaaga aattaccccg cattaaagcg ctgctaaccc ccggcgaagt
     1741 ggcgaaacgc agcggtgtgg cggtatcggc gctgcatttc tatgaaagca aagggttgat
     1801 taccagtatc cgtaatagcg gcaatcagcg gcgatataaa cgtgatgtgt tgcgatatgt
     1861 cgcaattatc aaaattgctc agcgtattgg cattccgctc gcaaccattc gtgaagcgtt
     1921 tggcgtgttg ccagaagggc atacgttaag tgcgaaagag tggaaacagc tttcgtccca
     1981 atggcgagaa gagttggatc ggcgcattca taccttagtg gcgctgcgtg acgaactgga
     2041 tggatgtatt ggttgtggct gcctttcgcg cagtgattgc ccgttgcgta acccgggcga
     2101 ccgattagga gaagaaggta ccggcgcacg cttactggaa gatgaacaaa actaaagcgc
     2161 cacaagggcg ctttagtttg ttttccggtc tttgtctttc tctctatccc gctggtacac
     2221 aggagggttt cccccgacgt caacacacct cattcgagca cgtggtggag gttccggttg
     2281 gtgttgatgc tttaattgta tgttaccggc gtttcttcgc cagtgtaaaa gtacactttt
     2341 tagccgcaat atttttgtca tctcagacga ttttttatcg caatcctgaa cggtatacgg
     2401 ctcgataacg ctgcaatctt gcgcatcgac gataacgttt gcgcatcaat tgcctggttt
     2461 ttcatcgtca agacaataaa agagaaaaaa gcagcaaact tcggttgaaa aagccgctat
     2521 gatcgccgga taatcgtttg cttttttacc acccgttttg tatgcgcgga gctaaacgtt
     2581 tgcttttttt gcgacgcagc aaattgtcgc aaacctggag caggaagata acgtttcgct
     2641 ggcaggggat tgtccgccac gcatcttgac gaaaattaaa ctctcagggg atgttttctt
     2701 atgtctacgc catcagcgcg taccggcggt tcactcgacg cctggtttaa aatttcacaa
     2761 cgtggaagca ctgtccgtca ggaagtggtt gccgggttaa caacgtttct ggcgatggtc
     2821 tactcggtta tcgtcgttcc aggcatgttg ggtaaagcgg gcttcccgcc tgcggcagtt
     2881 ttcgttgcaa cctgtctggt tgccggactc ggttctatcg tgatgggtct gtgggctaat
     2941 ctgccgttgg cgattggttg cgccatctct ctgacggcgt ttaccgcatt cagtctggtg
     3001 ctggggcaac atattagcgt acctgtcgcg ctgggtgccg tgttcctgat gggtgtgctg
     3061 tttacggtaa tttctgccac gggtatccgt agctggattt tgcgcaactt gccgcacggt
     3121 gtggcgcacg gcacggggat tggtatcggt ctgttcctgc tgctgattgc tgctaatggt
     3181 gtcggtctgg tgattaaaaa cccacttgat ggtctgcccg tcgcgctggg ggatttcacg
     3241 acctttccgg tgatgatgtc gttggtgggg ctggcagtga ttatcggcct ggaaaaactg
     3301 aaagtccctg gtggcattct gctgaccatt atcggtatct caattgtcgg tttgatcttc
     3361 gatcctaacg tccatttctc cggcgttttc gccatgcctt cattgagcga tgaaaacggc
     3421 aattcactga taggcagcct ggacattatg ggtgcgctga atcctgtagt cctgccaagt
     3481 gttctggcac tggtgatgac ggcagtattt gatgccaccg gaactatccg tgccgtcgcc
     3541 ggtcaggcga acctgctgga taaagatggg cagatcatcg acggcgggaa agcactgacc
     3601 accgactcca tgagcagcgt tttttctggc ctcgtgggtg cagctccggc agcggtatac
     3661 atcgagtctg cggcgggtac ggcggcgggc ggtaaaaccg gtttgacggc tatcaccgtt
     3721 ggcgtgctgt tcctgctgat tctgttcctc tctccgctct cttacctcgt tccggggtac
     3781 gcaacggctc cggcgctgat gtacgttggc ctgctgatgc tgagcaacgt ggcgaaaatc
     3841 gactttgctg attttgttga tgcgatggcg ggtctggtta cggcggtatt catcgtgctg
     3901 acctgtaaca tcgtaacagg catcatgatt ggcttcgcga ctctggtgat tggtcgtctg
     3961 gtttccggcg aatggcgcaa gttgaacatc ggtacggtcg ttattgccgt ggcgctggtg
     4021 accttctatg cgggtggctg ggctatctaa tcttacatag gattctgaaa acgggtggca
     4081 atggctgccc gtttttattt tctccgcaca ttgtgtgagc ttttgcgata ttctgaaaaa
     4141 atgagaattc aggcataacg cctttctcag atcataaaaa acatcgcaaa cagggaacgc
     4201 atggaaatct tcttcaccat actgataatg accctcgtgg tctcgctgtc cggggtggtc
     4261 actcgtgtca tgccctttca gatcccgctt ccgcttatgc aaatcgccat cggtgcgcta
     4321 ctggcgtggc cgacgtttgg tttgcatgtg gagtttgatc ctgaactctt tttagtcttg
     4381 tttatcccgc cgttgctgtt cgctgatggc tggaaaacgc cgacccgtga atttctcgaa
     4441 catggacgag agattttcgg tctcgcgctg gcgctggtgg tggtcaccgt ggtcggcatt
     4501 ggctttctta tttactgggt ggtgccgggc attccgctga tccccgcctt tgcgctggcg
     4561 gcggtgcttt ctccgaccga tgctgtggcg ctctcgggga ttgttggcga agggcgcatc
     4621 ccgaaaaaaa tcatgggcat tttgcagggc gaagcgttga tgaatgacgc ctccggcctg
     4681 gtgtcgttga agtttgccgt agcagtggcg atggggacga tgatcttcac cgtcggcggt
     4741 gcgacggtcg aatttatgaa agtagccatt ggcggtattc tcgccggttt tgtggtgagc
     4801 tggctgtacg gtcgctcgct gcgattcctc agccgctggg gcggtgatga acccgcgacg
     4861 cagatcgtcc tgctgttcct gctgccattc gcttcttatc tgattgccga acatattggc
     4921 gtttccggca tcctcgctgc ggttgccgcc gggatgacca tcacccgctc cggtgtgatg
     4981 cgccgtgcgc cgctggcaat gcgcctgcgc gcaaacagta cctgggcgat gctggaattt
     5041 gtctttaacg gcatggtatt cctgctgtta ggtctgcagc tgccgggtat tctggagacg
     5101 tcgctgatgg cggcagaaat cgaccctaac gtcgaaatct ggatgctgtt taccgatatc
     5161 attctgattt atgcggcgct gatgctggtc cgtttcggct ggctgtggac gatgaaaaag
     5221 ttcagcaacc gcttcctgaa gaagaagccg atggagtttg gttcgtggac cacacgagaa
     5281 atcctgatcg cgtctttcgc cggggtgcgt ggggcgatca ctctggctgg tgtgctctct
     5341 atcccgctgc tcttgccgga tggtaacgtc ttcccggcgc gctatgagct ggtgttcctg
     5401 gcggctggcg tcattctctt ctcgttgttt gtcggcgtgg tgatgttgcc tattctgcta
     5461 caacacatag aagtcgcgga ccattcgcaa caattgaaag aggaacgtat tgcacgagcg
     5521 gcaacggcag aagtggcgat tgtggcgatc cagaaaatgg aggagcgttt ggcggcggat
     5581 accgaagaga atatcgataa ccagctgctc acagaggtta gttctcgcgt cattggtaac
     5641 ctgcgtcgtc gcgccgatgg acgtaatgat gtagaaagtt caatcctgga agagaacctg
     5701 gagcgtcgct tccgtctggc ggcattgcgt tctgaacgtg ctgaactgta ccacctgcgc
     5761 gccacgcggg agatcagtaa cgaaacgctg caaaaattac tgcacgatct cgatttgctt
     5821 gaagcgttgc tgattgagga aaatcagtaa tctgcatggt tggcgggcga gcccgccaac
     5881 ccgacttact ctggcgttaa ccagtatcct tcacaacagc tcagccacgc gtgggcagag
     5941 tgggaaaggt aaacgccctc acgccaaatc attcctaatt tccagcttag ttcgctttcc
     6001 agcggtagcc attttaaggt gtttttatct aatcgctggc aaatgggttc cggcaatatg
     6061 gctattccga caccggcctg aaccatcgcg gcgagaaaat cccattgccc gctgcgaact
     6121 gcaattctcg gcttaacgcc atgttcatcg aataacttca tcaattgctt acttaaggca
     6181 aaatcttcgt tatagataag tagaggatgt tccgccaatg tctccggtga gacggcatca
     6241 cattcaagcc atttacctga atgtggcgtc agtacacata gtggatgact aaacagcgat
     6301 aacgttgtca ggccgctttc ttctactggc agggctgtca gtgccatatc cagttcaccg
     6361 ttcatcaccg cttgttgtac cgtcagacca ccaaactccg aaattttcaa ttctacgccg
     6421 gggtaacgct ggcgaaaaag gccaatcggc ccggccatca acatcccaac catcggcggg
     6481 attcccagcc gcaaaacgcc ggttttaagc tgattgatat cgctaagctc agcttccagt
     6541 tgccgaaact cagccagtaa agccagcccg cgctcaaata cgacgcgacc agtatcagtg
     6601 agaagcagcc gacgaccatc gcggatcaac aatatacagt tcagctcatc ttcgagattc
     6661 ttcagcatct tgctgatagt tggttgggtg acaaacaact tctccgctgc gcgggtgaag
     6721 ctttgctggc gcacgacttc gacaaaataa cgcagcgttc tgatatccat aactattccc
     6781 tttgactata cctgcaatga ttttaattca tttcagtcag gggcgagcgc tctctatact
     6841 taccattctg ttttattttc aggatatcct ctcatgcccg tggcgcttcg ccgcattacc
     6901 cctgccgtag tgcaatctat ccaggtattt tttcaggttg tgctgtatgc aggtttattt
     6961 atttttgcgc agtatctcgt ctcctggctg catttgccgc tgcctgccaa cctggtgggg
     7021 atgatcctga tgctggcgct gattgtttgt cgcattcttc ctctgcaatg ggttcgagcc
     7081 ggggcgcgtt ggctattggc cgaaatgttg ctgttttttg tccctgccgt ggtggctgtg
     7141 gtgaactatg cacaactatt actggtggat ggctggcgta tttttgctgt gatcgcgctg
     7201 agtacggtga tggtgctggg cacgactgcc tgggtagtgg aaaaagtcta tcgctatgaa
     7261 atgcgcaggt taaaccgtgg ctaattttca gctaagtatg ctctgtctgg caattacgct
     7321 agttatctat tttcttaaca aacgccttta tcgccgcttt cgtaaattgc cattgatgcc
     7381 tctggtgctg acgcctacgc tactggttat gctgctggtt tttggcaata tttcctggca
     7441 aaactatatt ggcgaagcgc actggttact atggctatta ggtccggcca ccatcgcctt
     7501 tgcggtacca gtgtatgaca atcgggcgat cattaagcgt cactggatgt cgttgacggc
     7561 aggcgtactg acggcaacgg tggtggcagt gaccagctca gtgtggctgg cgcgattgtt
     7621 taccctgcca gatgagatcc agcgtagcct ggcggtgcgt tcggtcacta cgccatttgc
     7681 tttggcagcg agccagtcgc tcggcggaca gcctgatctg gtggcgctgt ttgtggtggt
     7741 caccggtgtg tttggcatgg cgattggcga catgttgttt ttacgtctct ctattcgcga
     7801 agggatggcg aaaggtgctg gattcggcgc ggcatctcat ggtgccggga ctgcgcggtc
     7861 gtatgaactg ggtcaacaag aaggcgtagt tgcaagcctg gtcatgatgc tttcaggtgt
     7921 attgatggta ttggtttcgc cgctggtggc cagggtgatg ttctgaagaa attccccgat
     7981 caagcgaccg gggaagggga gagattaatg cgcgcggcct tgctcaacgc caaagccagt
     8041 ctgggagcgg ataaactgcg cacggaacag ttcacgctcg cgcgcacctt ccgctgaatt
     8101 atccgttgct gagaagaacc agataccgag gaatgccacg ctgatagaga acagcgccgg
     8161 gtattcatac gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tagtcgggcc
     8221 gaggatcatc agcaccaccg cagtaatcag tcctaaccag ccgcccagca ttgcgccacg
     8281 ggtagtcagt ttcgaccagt acatggaaag cagaatgatc gggaagttac agctcgccgc
     8341 gatggcaaac gccagcccca ccataaaggc gatgttctga ttctcaaaca gcacgccgag
     8401 gataatggca atcacaccga ggatcagtac ggtgattttc gatacccgca gctcttcacg
     8461 ttcggtcgca ccttttttga agacgttagc gtacaggtcg tgagacaccg ccgatgcgcc
     8521 cgccagcgtc agacccgcaa ccaccgcaag aatggtggcg aaagcaaccg ctgaaataaa
     8581 gccgaggaac aggttgccgc ccaccgcatt ggccaggtga actgctgcca tgttgttacc
     8641 accaatcaga tggcccgccg cgtctttata ttccggattc gcaccgacca gcatgatcgc
     8701 gccaaagccg ataataaagg tcaggatgta gaagtagccc ataaatccgg tggcgtagaa
     8761 cacgctctta cgtgcttcgc gggcatcgct gaccgtaaag aagcgcatca gaatatgcgg
     8821 caagcccgcc gtaccaaaca tcagtcctag ccccaaagag agcgcggaga taggatcttt
     8881 caccagcccg cccggcttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct
     8941 gaacagattg ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc
     9001 accgaacagc aacagcacgg ctttaataat ttgtacccag gtggtcgcca gcatgccgcc
     9061 gaacaggacg tacatcatca tcagcacgcc gaccagcact accgcaatgt gatagttaag
     9121 gccaaacagc agctcgatca gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc
     9181 caccaccacc agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacggta
     9241 agaggccaca tcggcaaagg tgtagcgacc gaggttacgc agacgttcgg caatcaggaa
     9301 cagaatgatc ggccagccca ccaggaagcc cagcgaataa attaagccgt catagccgga
     9361 ggtaaacacc agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat
     9421 cgccagcccg ttctgaaaac cggtgatatt gccgcctgcg gtgtagtagt cattacgcga
     9481 acgtacgcgt tttgacgccc agtaggtaat gccgagcgta aacacaacga aaatcaggaa
     9541 cataataata gcctgccagt tcgttggctg acgctctacg gccccgctaa tagcatccgc
     9601 ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct
     9661 tgtacctcat gcaggacttc gttattaaga cggtcgaatt cgccgttcgc ccgccagatg
     9721 tagataccag tgaggacaaa ggagatcaca attaccccga caccgactgg aataccgcgt
     9781 gtgacgctgg tgttcggatt cagcggggtg cccagccagc cgggcgcgaa ggcgatcagt
     9841 aaaataaagc cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc
     9901 cgtttttcga ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatcgtacca
     9961 ttcatcacag attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg
    10021 agaaggttaa cgcttgggta acccttgccg aatgtaggcc ggataaggca ttcacgccgc
    10081 atccggcaat caatgcctga tgcgacgctg tcgcgtctta ttaggcctac agccgttgcc
    10141 gaatgtaggc cggataaggc gttatacgcc gcatccggca atcaatgcct gatgcgacgc
    10201 tgtcgcgtct tatcaggcct acagccgttg ccgaatgtag gccggataag gcgttcacgc
    10261 cgcatccggc aaccagcgcc tgatgcgccg ctgtcgcgtc ttatcaggcc tacaaaccgt
    10321 taccgactcg catccggcaa ttgtgggtta cgatggcatc gcgatggcct gcttctcttc
    10381 aagcagcttc tcgactacgc caggatcggc aagcgtcgag gtatcgccca ggttgctggt
    10441 atcgcccgcc gcaattttgc gcagaatacg gcgcataatt ttgccggagc gggttttagg
    10501 cagggagtcg gtccagtgca gcacgtctgg cgtcgccagc gggccaatct ctttacgcac
    10561 ccagttgcgg acttctgcgt acagttctgg tgacggttcc tccccgtgat taagcgtgac
    10621 gtaggcgtag atcgcctgac ctttaatatt gtgcggaata ccgaccacag cggcttcggc
    10681 aattttcgga tgcgccacca gcgccgactc aatctctgcc gtccccaggc ggtgaccgga
    10741 gacgttcagc acgtcgtcca cgcgcccggt gatccagtaa tagccatctt catcgcgacg
    10801 cgcgccgtcg ccgctgaaat acatattttt gaaggtggag aaataggtct gctcaaaacg
    10861 ttcgtgatcg ccaaacagcg tacgcgcctg acccggccag gagtcagtaa tcaccaggct
    10921 gccttcggtg gccccttcca gcgggtgacc ttcgttatcg accagcgccg gttgcacgcc
    10981 gaagaacgga cgtgttgccg aaccggcttt tagctcggta gcgccaggca gcggggtgat
    11041 catgaaaccg ccagtttctg tctgccacca ggtgtcgacc accgggcatt tctcgttgcc
    11101 gatttttttc cagtaccact cccacgcttc cgggttaatt ggctcgccca cggaaccgag
    11161 aatgcgcagc gacgaacggt cggtgccttc gatcgcttta tcgccttccg ccatcagcgc
    11221 gcgaatcgcc gtgggcgcgg tatagagaat attgacctga tgcttgtcca ccacctgcgc
    11281 catacgggca ggcgtcggcc agttcggtac gccttcaaac atcagcgtgg tcgcgccgca
    11341 ggccagcggg ccatacagca aataactgtg tccggtaacc cagcccacat cggcggtgca
    11401 ccagtagatg tcgcccggat gataatcaaa gacatattta aaggtcagcg ccgcgtacac
    11461 cagataaccg ccggtagtgt gcagcacgcc ttttggctta ccggtagaac cggaggtata
    11521 gagaataaac agcggatctt cggcgttcat ctcttccgcc tggtgctgag cgctggcttg
    11581 ctcaaccagg tcgtgccacc acaggtcgcg cccttcctgc cagtcaattt ttccgccagt
    11641 acgcttcagt accaccacat gctctacgct ggtgacgttc gggtttttca gcgcgtcatc
    11701 aacgtttttc ttcagcggaa tactacgtcc ggcacgcaca ccttcgtcgg aagtgatcac
    11761 cagtcgtgaa ctggagtcga taatgcgtcc ggcaacggct tccggcgaga agccgccgaa
    11821 aatcaccgaa tgtaccgcgc caatgcgggc acaggccagc atcgcgaccg cggcttccgg
    11881 taccatcggc atataaatcg caactacgtc acctttttta atgcccagtt cgagcagggt
    11941 attggcgaag cggcagacgt cgcggtgcag ctctttatag ctaatatgtt tgctctggct
    12001 ggcgtcgtcg ccttcccaga tgatggcggt acgatcgccg ttttcttgca gatggcggtc
    12061 aaggcagttt gccgccagat tcagcgtgcc gtcctcgtac catttaatgg acacattacc
    12121 gggggcaaag gaggtgtttt tcaccttctg gtacggtgtg atccagtcga gaatttttcc
    12181 ctgttcgccc cagaaggtat ctggtgcgtt aatagattgt tgatacatcg cctcgtactg
    12241 ctgagggttt atcaggcaac ggtctgcgat gttggcagga atggtgtgtt tgtgaatttg
    12301 actcatgctt ttgttctcct tgtaagatgt taataatatg tggcataagc gttaaatgta
    12361 ggggtattgg cagttttgtt tagtatttgg gcgacagatc acgcaaaagt agaattgtgc
    12421 aaataaacgg cagggtaatt tttgaaggtc agcaacaaaa gttgattaat tctttgagga
    12481 acatgcagtt atgcatgctg ttgaaaaaga ggaagatact gactaactct aaagtggtat
    12541 tttacatgca cttacaattg attaaagaca acattcatag tgtggttatt tgttacacat
    12601 agggacgagc aatgtcatga cagtgtaggt gcggttactg tcgtgaaaaa caataaaaac
    12661 cgccattgca acaatggcgc aattcggatg aagccctatg acaaggataa aaataaacgc
    12721 acgccgtatc ttcagcttat tgattccttt tttctttttc acttctgttc acgctgaaca
    12781 aacggctgct cccgcaacac ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca
    12841 gcatcccgat caatatctct cctggaaagc tacctcggaa cagtcagagc gtgttgacgc
    12901 cctggcggaa gatccacggc tggtgatcct gtgggcaggg tatcccttct cgcgcgatta
    12961 caacaagccg cgtggacatg cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg
    13021 tgcgccgaaa aacgctgaag atggtccgct accgatggcg tgctggagtt gtaaaagccc
    13081 ggatgtggcg cgtctgatcc agaaagacgg cgaagatggc tacttccacg gaaaatgggc
    13141 gcgcggcggc ccggaaatcg tcaacaactt aggttgtgcc gattgccata acaccgcctc
    13201 accagagttc gccaaaggca aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc
    13261 gatggaagcc attggtaaac ctttcgagaa agccggacgt ttcgaccagc aatcgatggt
    13321 ttgcggtcag tgccatgtgg agtattactt cgacggcaaa aacaaagcgg ttaaattccc
    13381 gtgggatgac ggcatgaaag tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc
    13441 tgactggact aactccctgt cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga
    13501 aacctggaca gcgggcattc acggcaaaaa caacgtgacc tgtatcgatt gccatatgcc
    13561 aaaagtgcag aacgccgaag gcaaactcta caccgaccat aaaattggta atccgtttga
    13621 taacttcgcc cagacttgtg cgaactgcca tacccaggac aaagctgcct tgcaaaaagt
    13681 ggtcgcggaa cgtaagcagt cgattaacga cctgaaaatc aaggttgaag atcaactggt
    13741 tcacgctcac ttcgaagcga aagcggcgct ggatgcaggc gcaacggaag ccgaaatgaa
    13801 gccgattcag gacgatatcc gtcatgccca gtggcgttgg gatttggcga tcgcttccca
    13861 cggcattcat atgcacgcac cggaagaagg tctgaggatg ctcggtacgg cgatggataa
    13921 agcggcggat gcacgcacca aactggcgcg cctgctggcg accaaaggca taacccatga
    13981 aatccagatc ccggatatct cgaccaaaga gaaagcccag caggccattg gcctgaacat
    14041 ggagcaaatc aaggccgaga agcaggactt catcaaaacg gtgatcccgc agtgggaaga
    14101 gcaggcacgt aaaaacggtc tgttaagcca ataaccccgt tccgcctcgc aaggggcgga
    14161 aaacacaatg gagtgaatat gagcgtatta cgttcgttat taactgccgg ggtgctggcg
    14221 tcgggcctgt tgtggagcct gaacggaatt accgctaccc ctgcggcgca ggcatctgat
    14281 gatcgctacg aagttaccca gcagcgtaac ccggatgccg cctgtctgga ctgtcataag
    14341 ccagataccg aaggtatgca tggcaaacat gcttctgtta tcaacccgaa taacaaactg
    14401 ccggtcacct gcaccaactg ccacggtcag ccatcaccgc aacaccgcga aggggtgaag
    14461 gatgtgatgc gctttaacga gccgatgtac aaggttggag agcagaacag cgtctgtatg
    14521 tcctgccatc tgccggaaca gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc
    14581 aaagtggcgt gcgccagctg ccattccctg catccacagc aagatacgat gcagacgtta
    14641 agtgacaaag gacggattaa gatttgcgtc gattgccaca gcgatcagcg caccaatccg
    14701 aactttaacc cggcgtctgt tccgttgctt aaggagcagc catgacctgg tctcgtcgcc
    14761 agtttctcac cggcgtcggc gtgctggcag ccgtcagcgg caccgcagga cgcgtggtgg
    14821 cgaaaacgtt gaatatcaat ggggtgcgtt acggcatggt gcatgacgag tcgttatgca
    14881 tcggctgtac cgcctgtatg gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct
    14941 cgcgcctgac gattattcgt agcgagccgc agggcgaatt tcctgatgtg aaatatcgct
    15001 tcttccgtaa gtcttgccag cactgcgatc atgcgccgtg cgttgacgtt tgcccgaccg
    15061 gtgcgtcttt tcgcgacgct gccagtggga ttgtcgatgt taatccggat ctctgcgtcg
    15121 gttgtcagta ctgcatcgcc gcctgtccgt accgcgtgcg ctttatccat ccggtcacga
    15181 agacggcgga caaatgcgat ttctgccgta agacccattt gcaggccggt aagctgcccg
    15241 cgtgcgttga ggcttgcccg acgaaggcgc tgacgtttgg caatctggac gatcccagca
    15301 gtgagatttc gcaactgttg cgccagaagc ccacttaccg ctacaagctg gcgctgggaa
    15361 ccaaaccgaa gctgtaccgc gtaccgttta aatacgggga ggtgagccaa tgacgcagac
    15421 ttccgcattt cattttgaat cgctggtgtg ggactggccg attgccatct acctgttttt
    15481 gattggtatt tctgccggtc tggtgacgct ggccgtgctg ttacgtcgtg tctacccgca
    15541 ggcgggcggt gcagacagta cgttgttgcg caccacgctg attgtcgggc ctggcgcggt
    15601 gatcctcggt ctgttgatcc tcgtcttcca cctgacaaga ccgtggacct tctggaagct
    15661 gatgttccac tacagtttta cctcggtgat gtcgatgggg gtgatgctgt ttcagcttta
    15721 catggtggtg ctggtgctgt ggctggcgaa aatctttgaa catgatttgc ttgccctgca
    15781 acaacgctgg ttgccgaagc tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt
    15841 tcatcgcgga ctggaaacat tgatgctggt gctggcggtg ttgttggggg cttataccgg
    15901 ctttctgctg tcggcgctga aatcgtatcc gttcctcaat aacccgatcc tgccggtgct
    15961 gttcctcttc tccggcatct cgtctggtgc ggcggtggcg ctgatcgcca tggcgatacg
    16021 ccaacgcagt aacccgcatt ccacggaagc gcactttgta caccgtatgg aaatccccgt
    16081 ggtatggggt gaaatcttcc tgctggtggc gttttttgtc ggtctggcgc tgggcgatga
    16141 cggtaaagtg cgtgcgctgg tggcggcatt gggcggcggt ttctggacgt ggtggttctg
    16201 gcttggtgtc gccgggctgg ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg
    16261 cagttccggc attcctgccg tgctggcggc atgtggggcc agcctggtcg gcgtgttgat
    16321 gctgcgcttt ttcattctct acgccgggca gttgacggtg gcgtaagcca gaaaagaggt
    16381 ggtttctgga cgtattcctt cctgaagtcg gttttctggc gttgttgtta agtctcgggg
    16441 tcaacgtgtt gaccccgttg acggccttcg cgggagtgcg gttgcgctgg cctgccatga
    16501 tgcgactcac ttgcatcggc attctggcgc agttcgcgct cctgctgctc gcctttggcg
    16561 tactgacgta ttgttttctc atcagcgatt tctcggtcat ttatgtcgcc caacatagct
    16621 atagcctgct gtcgtgggaa ctcaaactgg cggcggtgtg gggcggtcat gaaggttcgc
    16681 tgctgctttg ggtgctgctg ctttccgcct ggagcgtgct gtttgcctgg cattatcgac
    16741 agcaaaccga tccgctattt ccgctcacgt taacggtttt atcgctgata cttgccgcac
    16801 tgctgctgtt tgttgtcgga tggtccgatc cttttgtgag gatatttccg cccgcaatag
    16861 aagggcgcga tctcaaccca atgctgcaac atcccggtct tatctttcat ccaccgctgc
    16921 tttatctcgg ctacggcggt ttgatggtgg cggcgagcgt ggcgctggcg agtttactgc
    16981 gcggcgagtt tgatggtacc tgcgcccgaa tttgctggcg ttgggcacta cctggctgga
    17041 gcgcattaac cgcagggatc attctcggtt cctggtgggc ttattgcgaa ctgggctggg
    17101 gcggttggtg gttttgggat ccggttgaaa acgcgtcgtt attaccctgg ctttcatcta
    17161 ctgcgttgct acacagtttg tctttgacac gccagcgggg gattttccgc cactggtcgc
    17221 tgttgctggc gatagtaact ctgatgctgt cgctgctggg caccttaatt gtccgttctg
    17281 gcattctggt ttcggttcat gcgttcgcgc tggataacgt ccgcgccgtg ccgttgttca
    17341 gcctgtttgc actgattagc cttgcgtctc tggctctgta tggctggcga gcgcgggacg
    17401 gtggcccggc ggtgcgtttt tcggggttat cgcgggaaat gttaatcctc gctacgctgt
    17461 tgctgttttg cgcagtgcta ctgatcgtgc tggtgggaac gctttatccg atgatttacg
    17521 gcctgctggg ctggggacgc ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac
    17581 cgtttgctct gttgatgctg gtggtgattg tgctggcgac gtttgtctct ggcaaacgtg
    17641 ctcagcttcc ggcgctgctg gcgcatgcgg gcgtgctgtt atttgccgcg gggattgtgg
    17701 tttccagcgt cagccgtcag gagatcagcc tgaatttaca gccgggtcag caggtgacgc
    17761 tggcaggata caccttccgt tttgagcgcc tcgatctgca agccaaaggc aattacacca
    17821 gcgaaaaagc gatagtggca ctgtttgacc atcagcaacg cattggtgaa ttaacgccgg
    17881 agcggcgttt ttacgaagca cgtcgtcagc aaatgatgga accgtcaatt cgctggaatg
    17941 gcatccatga ctggtatgcg gtcatgggcg agaaaaccgg agcggatcgt tacgcttttc
    18001 gtttgtatgt acaaagcggt gtgcgctgga tctggggggg aggattgttg atgatttcgg
    18061 gcgcattgtt aagcggatgg cggggaagga agcgcgatga ataaagggct tctcacgcta
    18121 ctgctgttat ttacctgttt tgcgcgcgct caggttgtag acacctggca attcgccaat
    18181 ccgcaacaac agcaacaggc gttaaatatt gccagccagt tacgttgtcc gcagtgccag
    18241 aatcagaact tactggaatc caatgcgccg gtggcagtca gtatgcgcca tcaggtttac
    18301 accatggtgg aagaggggaa aagtgaagtc gaaatcattg gctggatgac cgaacgctac
    18361 ggagattttg ttcgctataa cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg
    18421 ccagtagtgt tgttactgct gatggcactg atcctctggc gagtgagggc gaagcgatga
    18481 aacagcccca aataccggtg aaaatgctga caacactcac gattttgatg gtatttctct
    18541 gtgtcggcag ttatctgtta agtccaaaat ggcaggcggt acgtgcggag tatcagcgtc
    18601 agcgcgatcc gctgcatcag tttgccagcc agcaaacccc ggaagcgcag cttcaggcat
    18661 tgcaggataa aatccgtgct aatccacaaa acagcaaaca gtgggcgtta ctgggcgagt
    18721 attatctgtg gcaaaacgat tacagcaatt cgctgctggc gtaccgtcag gcgttgcaac
    18781 tacgtggtga gaacgctgaa ctgtatgcgg cgctggcgac ggtgctttat taccaggcca
    18841 gccaacatat gaccgtccag actcgcgcaa tgatcgacaa agccctcgcg ctggactcta
    18901 acgaaatcac cgctctgatg ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc
    18961 aagccatcga actatggcaa aaagtgatgg atctcaactc accccggatc aaccggacac
    19021 agctggtcga gtcgattaat atggcgaaat tgttgcagcg gagatcagat taatcatctg
    19081 gtg
//
LOCUS       DAZFRG010000082        18901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFRG010000082 DAZFRG010000000
VERSION     DAZFRG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.082"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..1036
                     /locus_tag="ACHKUC_002222"
     CDS             83..1036
                     /locus_tag="ACHKUC_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6618476.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1077..2072)
                     /gene="pgl"
                     /locus_tag="ACHKUC_002223"
     CDS             complement(1077..2072)
                     /gene="pgl"
                     /locus_tag="ACHKUC_002223"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO6618477.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2227..3045
                     /gene="ybhA"
                     /locus_tag="ACHKUC_002224"
     CDS             2227..3045
                     /gene="ybhA"
                     /locus_tag="ACHKUC_002224"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO6618478.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3046..4104)
                     /gene="modC"
                     /locus_tag="ACHKUC_002225"
     CDS             complement(3046..4104)
                     /gene="modC"
                     /locus_tag="ACHKUC_002225"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO6618479.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLALLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4107..4796)
                     /gene="modB"
                     /locus_tag="ACHKUC_002226"
     CDS             complement(4107..4796)
                     /gene="modB"
                     /locus_tag="ACHKUC_002226"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO6618480.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4796..5569)
                     /gene="modA"
                     /locus_tag="ACHKUC_002227"
     CDS             complement(4796..5569)
                     /gene="modA"
                     /locus_tag="ACHKUC_002227"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6618481.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5736..5885)
                     /gene="acrZ"
                     /locus_tag="ACHKUC_002228"
     CDS             complement(5736..5885)
                     /gene="acrZ"
                     /locus_tag="ACHKUC_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO6618482.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6014..6802
                     /gene="modE"
                     /locus_tag="ACHKUC_002229"
     CDS             6014..6802
                     /gene="modE"
                     /locus_tag="ACHKUC_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO6618483.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPINEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6870..8342
                     /gene="modF"
                     /locus_tag="ACHKUC_002230"
     CDS             6870..8342
                     /gene="modF"
                     /locus_tag="ACHKUC_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO6618484.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASHQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDVLGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GEMQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8604..9620
                     /gene="galE"
                     /locus_tag="ACHKUC_002231"
     CDS             8604..9620
                     /gene="galE"
                     /locus_tag="ACHKUC_002231"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6618485.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9630..10676
                     /gene="galT"
                     /locus_tag="ACHKUC_002232"
     CDS             9630..10676
                     /gene="galT"
                     /locus_tag="ACHKUC_002232"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO6618486.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10680..11828
                     /gene="galK"
                     /locus_tag="ACHKUC_002233"
     CDS             10680..11828
                     /gene="galK"
                     /locus_tag="ACHKUC_002233"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO6618487.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11822..12862
                     /gene="galM"
                     /locus_tag="ACHKUC_002234"
     CDS             11822..12862
                     /gene="galM"
                     /locus_tag="ACHKUC_002234"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO6618488.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13065..13817
                     /gene="gpmA"
                     /locus_tag="ACHKUC_002235"
     CDS             13065..13817
                     /gene="gpmA"
                     /locus_tag="ACHKUC_002235"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO6618489.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13984..15036)
                     /gene="aroG"
                     /locus_tag="ACHKUC_002236"
     CDS             complement(13984..15036)
                     /gene="aroG"
                     /locus_tag="ACHKUC_002236"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO6618490.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15352..15732
                     /locus_tag="ACHKUC_002237"
     CDS             15352..15732
                     /locus_tag="ACHKUC_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO6618491.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNIDNNGVNTGSDGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15846..16787
                     /gene="zitB"
                     /locus_tag="ACHKUC_002238"
     CDS             15846..16787
                     /gene="zitB"
                     /locus_tag="ACHKUC_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO6618492.1"
                     /translation="MAHSHSHASSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16784..17503)
                     /gene="pnuC"
                     /locus_tag="ACHKUC_002239"
     CDS             complement(16784..17503)
                     /gene="pnuC"
                     /locus_tag="ACHKUC_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO6618493.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17541..18584)
                     /gene="nadA"
                     /locus_tag="ACHKUC_002240"
     CDS             complement(17541..18584)
                     /gene="nadA"
                     /locus_tag="ACHKUC_002240"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO6618494.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4770 a         4937 c         4708 g         4486 t
ORIGIN      
        1 attaatttaa gttggctaaa tgcagttaaa atttttaact gccagtcacc caaaatcgtc
       61 gcttccattt tccggagtaa taatgaagca tgaattatca agtatgaagg catttgtcat
      121 actggcagag tccagttcat ttaacaatgc tgctaaatta ctcaatatta cgcaacctgc
      181 attaacgcga agaataaaaa agatggagga agatttacac attcaactat ttgagcgtac
      241 aactcgcaag gttacgttaa caaaagcagg aaaaaggttg ctcccggagg cgcgggaatt
      301 aataaaaaaa ttcgatgaaa cgctttttaa tattcgtgat atgaatgctt atcatcgtgg
      361 tatggtgacg ttagcgtgta ttccgaccgc agtattttat tttttaccgc tggcaatcgg
      421 taaatttaat gagctgtatc ccaatattaa agtgcggatt ctggaacaag gcacgaataa
      481 ttgcatggaa tcggtgctgt gtaacgaatc agactttggc ataaacatga acaacgtcac
      541 aaattcatcc attgatttta ccccactggt caacgagccg ttcgtgctgg cctgtcggcg
      601 tgaccatcca ttggccaaaa agcagcttgt agaatggcag gagttagtgg gttacaaaat
      661 gattggcgta cgctcctcca gcggcaaccg actattgata gaacaacagc tagccgacaa
      721 gccctggaag ctggactggt tttacgaagt gcgtcatctt tcgacgtcat tagggctggt
      781 ggaggcggga ctggggattt cggcgcttcc tggtctggca atgccccatg cgccctattc
      841 ttcgattatt ggtatcccgc tggtggaacc ggttatacgt cggacattgg ggattattcg
      901 ccgcaaagat gccgtgcttt ctccggcagc tgaacgcttt tttgccttgc tgattaattt
      961 gtggactgac gataaagaca atttgtggac caatatcgtc gaacgccaga gacatgcgtt
     1021 acaggagata ggttaataca ccggcgcaga agagactgcg ccgggtaaat cagcggttag
     1081 tgtgcgttaa ccaccaccca cattggtccc tgcccgaccg catagcggcc tttttcatgc
     1141 agtagcccct gctcgccaac aatttcgtat accgagatgt ggtgagattt ttgcccggcg
     1201 gcaatcagat acttgccgct gtgatcaaca ttgaagccgc gcggctgggt ttcagttggc
     1261 tggaagcctt ctttactcaa cacgctgcca tcttccgaaa cgctgaaaac agtaatcaga
     1321 ctggcggtac ggtcgcaggc gtataaatga cgaccatccg gggtgatatg aatatcagcc
     1381 gcccaacggg tgtcggagaa gttttccggc atcatatcca gcgtctggac acattcgata
     1441 ttgccatgcg gatctttcag ttcccagaca tccactgagc tgtttaactc attgacgcaa
     1501 tacgcatatt gttcgtttgg atggaatacc atatggcgtg ggccggcccc ttcaacggta
     1561 gtcacttccg cagggtcctg cgctaccaga tgaccatcat cgctgaccgt aaacaggcaa
     1621 atgcgatcct gctttaatgc cggaacccac aacgtacggt tgtccggtga gatattggcg
     1681 gaatggcaac cgtccagccc ctcgaccaca tcgacgacgc ccactggcag gccatcttcc
     1741 agacgcgtta cgctcacgtt acccgcattg taagaaccga caaagacaaa ctgcccctgg
     1801 tgatcggtgg aaatatgcgt cgggctaccc ggcagcgcag actctgcagc aaaggtcagc
     1861 gcgccatcat ccggtgcaat acgatacgcc agaacacgaa actccggacg gacaccaacg
     1921 tagaggtaac gtttgtccgg gctgaccacc atcggctgca cctgccccgg cacatcgact
     1981 acctgtgtca gcgtcagtgc gccttcatga ttcaggttcc agacgtgaat ttgctggctc
     2041 tcagggctgg cgatataaac tgtttgcttc atgaatgctc ctttgcattt agcaactgac
     2101 tgtaacagct aaaattagtc gcttttggcg gtgaatgctg aattaaaacg cagaattttg
     2161 tccgatagtg gtatcggtgt accattcgcg cagacgaaaa ttcttaacct taatctggaa
     2221 tacgccatga ccacacgcgt gattgctctc gacttagacg gcaccttatt gaccccgaaa
     2281 aagaccctgc ttccttcatc gatagaagcc ctggcccgtg ctcgcgaagc aggctaccga
     2341 ttaatcatcg tcacaggtcg ccatcacgtc gctattcatc ctttttatca ggcgctggcg
     2401 ctggatacac ctgctatttg ctgtaacggc acctatttgt atgattatca tgcaaaaacc
     2461 gtactagaag cggacccaat gcctgttaac aaagccctgc aactcattga gatgctgaat
     2521 gaacaccaca ttcacggtct gatgtatgtg gatgatgcga tggtttatga gcacccgacc
     2581 ggacatgtca ttcgcacgtc taactgggcg caaaccctgc cgccggaaca gcgtccgact
     2641 ttcacacaag tcgcttctct ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc
     2701 gccctgacgc acgatgacct gccgcaattg cagaattttg gtaagcatgt cgaacatgaa
     2761 ctgggactgg agtgtgaatg gtcctggcac gatcaggttg atattgcacg cggcggcaac
     2821 agcaaaggta aacgtttgac gaaatgggtt gaggcgcaag gctggtcgat ggaaaacgtc
     2881 gtggccttcg gcgataactt caatgatatt agtatgctgg aagccgctgg tacaggcgtg
     2941 gcgatgggca acgccgatga cgcagtaaaa gcgcgcgcca acattgtgat tggtgataac
     3001 accaccgaca gcattgccca gttcatttat agccacctga tttaatcagg cggttatcga
     3061 cacactttta atttgcgcat acagccacag gccaggtttg atcgccagtt catccctggc
     3121 ccacgggctg attcgcgccc acagcgtttt accgccgact tccagctcca cttccacctg
     3181 gccgttgtcg tcataactat taacaacttt tgcccgcaat acgttacgaa tgctggtttg
     3241 ctgcggcggt tgtaaaacca gcgagacatc ggaagcctga atgcggatgc gtagcgcagc
     3301 ttgcagcggt tcgtccagct tattgaccca caaatgctga tcgcccagcg ccagcgcggt
     3361 catcgcgtaa tgcggatgat gctcaagtac tgtcactttc agaatgctac tttgttgctc
     3421 tttcggtagc cacggattca tcacgctact accccagact tcttccagcg cgccaaaggc
     3481 tttcacctga ccgttttcca gtaccatcac tctgtctgcc agatggagga tttcatccag
     3541 cgaatggctg acatacaaca tcggaatgtt gatttcccgc gtcagacgtt gcagataagg
     3601 caacagttcg cgtttacgcg gaatatccag taacgccagc ggttcatcca gcaacagcaa
     3661 ttccggtgct gtcagcaaag cccgaccaat cgccacgcgc tgtttttcgc ctccggacag
     3721 gctgcctggt aaacgatcaa gcaacggttc aatgcctaaa agcgccacca gcttatcgaa
     3781 ctgattgacc atgcttttcg ccatgccgta gcgcaaattg ccacgcactt tataatgcgg
     3841 aaacagccgc gcatcctgaa acacatagcc tacgcgacgc ttttctggcg tcaggcagat
     3901 acctttatcg gcgtcattta gtacccgacc attgaggaca atccgccctt tttgcgggcg
     3961 cgtcagccca ctgatggcgt taatcagcga ggtttttccg gcaccagaga caccaaaaat
     4021 agcggtgatg ccattggcgg gcagcgtttc attaatcgtc aggcaatggt tgcccaacgt
     4081 ctgggaaaaa ttcagttcca gcatgattag cgccccgccc gctcacggct gattctggcc
     4141 agccattctg aaatcaacag ggagatcatc gccagcgcaa tagaaataat acacagtctc
     4201 gccgcgccac tttccccgcc aggggtctgg atcagggtat acatggcaga aggaatggtc
     4261 cgcgtttcac caggaatgtt cgacacaaag gtgatggttg cgccaaattc accgagcgaa
     4321 cgagcgaatg ccagtaccgt accaacaata attcccggca acgtcagcgg taaggtaata
     4381 gtgaagaaga cacgccagcg cccggcgccc aacgttcttg cggcctgctc cagtttgata
     4441 tcaacacctt ccagcgccag acgaatcgcc cgcaccatca gcggaaaaga catgacagcg
     4501 gcagcgagaa ccgcaccgcg ccagctaaac gcgaaagaaa gaccaaacca gtcatacaga
     4561 cgttcaccga taaatccgcg ccgtcccatc gaaactaata ataggtaacc gacgaccacg
     4621 ggcggtaaca ccagcggtag atgcagtaca ctgtcgagca gagctttgcc cggaaacgtg
     4681 caacgtacca gtaaccaggc aaaaaagatc ccaaacggca ggctaaacag cacagccagg
     4741 gaagaaactt tcaggcttaa taaaactgcc tgccattctg gatcggtcag tatcattact
     4801 tggttgtaaa tccgtaacgt ttaaagattt ccgcagcctg cggccccttc agataatcgt
     4861 aaaatgcttt cactgtcgca ttgttatgcc cttccacaac agcaaccgga tattccactt
     4921 ttttatgtga atcttccggg aaggtggcaa ccacttttac ccctttgctg gcaactgcgt
     4981 cagaaccgta aacaataccc agcggcgctt cattgcgttc gaccaacgcc agcgcgccac
     5041 gaacatcttc cgccggagcc agtttcgggg agagcgtatc ccatgcgccc agtttttgca
     5101 gtgcttcttt cgcataaatg ccagcaggga catgttccgg atcaccaacc gcgaggcgac
     5161 cgccattcag cagcgaagtc cagttggttt tgctgtcgat ggtgaaatct ttctgctcgc
     5221 tggctttcgg tgctacaacg accaggctat tgccgagcag tgtctggcgc gaagccgtat
     5281 cgatcgcttt tttatcaacc gcataatcca tccatttctg atcggcagaa ataaacagat
     5341 ccgcaggcgc acccgcttca atctgacggg ccaaagtaga tgacgaagcg aaagaagaaa
     5401 ctacatccac gcctttctct tttttatact gcgtagcaat gtcctgcatt gcgttagtca
     5461 gtgatgcggc ggcgaacacc gtgattttcc cttcatctgc cagtgcattg ccagcaacag
     5521 cgaaagagag tgccgccccg gtaaacaagt tcaaccattt acgagccatt ggtaacccct
     5581 taatgtaacg ttatgtaggc gtcaatataa cggcaagttc ggaattttcc cagcagttat
     5641 tgctaacctt tgtgaggtag atgagaaaaa atatcggcag aaaaagcagg aagttgagaa
     5701 aaagaaaatg cccgactaag cgggcattca gggaatcaat gattttgtcc gggctggtct
     5761 tttttaccaa caccagaaaa gatgttgaaa acttcaccaa gaccgtaaat cagacccagg
     5821 atgatggcca tcacgacagg taccatgatt acggcgaata ccagactttt taataactct
     5881 aacatggtca actccagata tagtcatgag actattctaa ccgctaagca cagaaaagca
     5941 ctcccctttt gtgcggtcag ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg
     6001 ccaggatatt gttatgcagg ccgaaatcct tctcaccctt aagctccaac aaaaattatt
     6061 cgccgacccg cgccgcattt cgctactaaa acacattgcg ctttccggtt ccattagcca
     6121 gggagcgaaa gatgccggta tcagctataa aagcgcctgg gatgccatta acgagatgaa
     6181 tcagttaagt gagcatattc tggttgagcg cgcaacaggc ggtaaaggtg gaggcggcgc
     6241 agtactgacc cgctatggtc agcgactgat tcagctctat gacttactgg cgcaaatcca
     6301 gcaaaaagcc tttgatgtgt taagtgacga tgacgccctg ccgctgaaca gcctgctggc
     6361 cgcgatctca cgtttttcac tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac
     6421 cgcccgcgat catgatgacg ttcaacagca tgttgatgtc ttactggctg atggaaaaac
     6481 acgcctgaaa gtcgcaatta ccgcacaaag cggcgcacgt ctggggctgg atgaaggcaa
     6541 agaagtgttg atattgctaa aagcgccgtg ggtagggatt actcaggacg aggcggtcgc
     6601 gcaaaacgct gacaaccaat taccgggtat tattagtcat attgagcgcg gcgcagagca
     6661 gtgcgaagta ttaatggcgc tacccgacgg gcaaacactg tgcgccacag tgccgataaa
     6721 tgaagcgact tctcttcagc aaggacagaa tgtcacggcc tactttaatg ccgacagcgt
     6781 gattatcgcc acgctgtgct aagcgtgttg acaatttgtt atgaaacacg tatccctgtc
     6841 agtaatcgct gcacaaaatg ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt
     6901 ttcgtcttgg cgacacaaaa acgctgcaat tgcctcggct aacgttaaac gcgggtgata
     6961 gttgggcgtt tgtcggttcg aatggaagcg ggaaatcggc gctggcccgc gcgctgtcgg
     7021 gggaacttcc gcttttgaaa ggtgaacggc aaagccagtt ttcccacatc actcgtctct
     7081 ccttcgagca attgcaaaag ctcgtcagcg acgaatggca gcggaataac accgatatgc
     7141 ttagtcctgg cgaagatgac accgggcgta ccacggctga gatcattcag gatgaagtaa
     7201 aggatactcc gcgttgcatg caactggcgc agcagttcgg tattaccgcc ctcctcgacc
     7261 gacgctttaa atacctttcc actggcgaga cgcgaaaaac cctgctgtgt caggcgctga
     7321 tgtcggagcc tgacttgttg atccttgatg aaccgttcga tggcctggat gttgcctcac
     7381 atcagcaact ggctgagcta ctcgcctcgt tacatcagtc cggtattact ctggtactgg
     7441 tgctcaatcg cttcgatgag atcccggaat ttgtccagtt tgctggcgtg ctggcggatt
     7501 gcacgttagc ggaaactggc gctaaagaag aactgctcca acaagcactc gttgcgcaac
     7561 tggcgcatag cgaacagctt gaaggtgtgc aactgccgga gccggatgaa ccttcagcac
     7621 gtcacgcctt acccgccaac gaaccgcgca ttgtgctgaa caatggcgtg gtttcttata
     7681 acgaacgccc cattcttaat aaccttagct ggcaggtgaa tccaggcgaa cactggcaaa
     7741 ttgtcgggcc aaatggcgcg ggaaaatcga cgttattaag cctgattact ggcgatcatc
     7801 cgcaaggtta cagcaacgat ttgacgcttt tcggacgacg tcgcggcagc ggcgaaacca
     7861 tctgggatat caaaaagcat atcggttacg tcagcagtag tttgcatctg gattaccggg
     7921 tcagcactac tgtgcgtaat gtgatccttt ctggctattt tgattcgatt ggcatttatc
     7981 aggccgtttc ggatcgccag caaaaactgg tgcagcagtg gctggatgtt ctcggcattg
     8041 ataaacgcac ggctgacgct ccgttccata gtctttcctg gggacagcag cgtctggcgc
     8101 tgatcgtccg cgcgctggtg aaacatccga cgttgcttat tctcgatgaa ccactacagg
     8161 ggcttgatcc gctcaatcgc cagcttatcc gccggtttgt tgatgtgctg attagcgaag
     8221 gtgaaatgca attgttgttt gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc
     8281 atcgtcttga gttcgtgccg gacggtgact tttatcgcta tgcgctgaca aaaataaact
     8341 gagtcggtag tgctgaccgt gccggaggcg gcgtaaacgt gttatccggc caatggtttg
     8401 acgcatgcag gcatgaaacc gcgtcttttt ttcagataaa aagcgcaatc attcataaac
     8461 cctctgtttt ataatcactt aatcgcacat taaaaacggc taaattcttg tgtaaacgat
     8521 tccactaatt tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata
     8581 ccataagcct aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg
     8641 gaagtcatac ctgtgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc
     8701 tctgtaacag taagcgcagc gtactgcctg ttatcgagcg tttaggcggc aaacatccaa
     8761 cgtttgttga aggcgatatc cgtaacgaag cgttgatgac cgagatcctg cacgatcacg
     8821 ctatcgacac cgtgatccac ttcgccgggc tgaaagccgt tggcgaatcg gtacaaaaac
     8881 cgctggaata ttacgacaac aatgtcaacg gcaccctgcg cctgattagc gccatgcgcg
     8941 ccgctaacgt caaaaacttt atctttagct cctccgccac cgtttatggc gatcagccca
     9001 aaattccata tgttgaaagc ttcccgaccg gcacaccgca aagcccttac ggcaaaagca
     9061 agctgatggt ggaacagatc ctcaccgatc tgcaaaaagc ccagccggac tggagcattg
     9121 ccctgctgcg ctacttcaac ccagttggcg cgcatccgtc gggcgatatg ggcgaagatc
     9181 cgcaaggcat tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg
     9241 actcgctggc gatttttggt aacgattatc cgaccgaaga cggtactggc gtgcgcgatt
     9301 acatccacgt aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca
     9361 agccaggcgt acacatctac aacctcggcg ctggcgtggg cagcagcgtg ctggacgtgg
     9421 ttaatgcctt cagcaaagcc tgcggcaaac cggttaatta tcattttgca ccgcgtcgcg
     9481 agggcgacct tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc
     9541 gcgtaacgcg cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc
     9601 cacagggata tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac
     9661 atcgccgcta caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc
     9721 gcccctggca gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatccag
     9781 attgcttcct ctgcgcaggc aatgtgcggg tgacaggcga taaaaacccc gattacaccg
     9841 gcacttacgt tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag
     9901 aaagcaacga tccgctaatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct
     9961 tttcaccgga tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg
    10021 tcaaaacctg gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtacaggtct
    10081 ttgaaaacaa aggcgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg
    10141 caaatagctt cctgcctaac gaagctgagc gcgaagaccg tctgcaaaaa gaatatttcg
    10201 ccgaacagaa atcgccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc
    10261 gtaccgttgt cgaaaccgaa cactggttag ccgttgtacc ttactgggct gcctggccgt
    10321 tcgaaacgct actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc
    10381 agcgcagcga tttggctctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc
    10441 agtgctcctt cccctactct atgggctggc acggcgcgcc atttaatggc gaagagaatc
    10501 aacactggca gctgcacgcg cacttttatc cgcctctgtt gcgctctgcc accgtacgta
    10561 aatttatggt tggttatgaa atgctggcag aaacccagcg agacctgacc gcagaacagg
    10621 ccgcagagcg tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa
    10681 tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc
    10741 acaccattca ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg
    10801 gtttcgttct gccctgcgcg attgattatc aaaccgtgat cagctgtgcg ccacgcgatg
    10861 accgtaaagt tcgcgtaatg gcagccgatt atgaaaatca gctcgacgag ttttccctcg
    10921 atgcgcccat tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga
    10981 aacatctgca actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg
    11041 tgccgcaggg ggccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat
    11101 tgcagcagct ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag
    11161 cagaaaacca gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg
    11221 gcaagaaaga tcatgccttg ctgattgact gccgctcact ggggaccaaa gcagtttcca
    11281 tgcccaaagg tgtggctgtc gtcattatca acagtaactt caaacgtacc ctggttggca
    11341 gcgaatacaa cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag
    11401 cgctgcgcga tgtcaccatt gaagagttca acgctgttgc gcatgaactg gacccgatcg
    11461 tggcgaaacg cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg
    11521 cgctggagca aggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta
    11581 tgcgcgatga tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag
    11641 ctgtgattgg cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg
    11701 tcgcgctgat cccggaagag ctggtgcctg cagtacagca agctgtcgct gaacaatatg
    11761 aagcaaaaac aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac
    11821 agtgctgaac gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt
    11881 gcgtaacaac gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttccgc
    11941 ccgtattccg ctttccgatg gcagcgtccg cgaggcgctg cttggctgcg ccagcccgga
    12001 atgctatcag gatcaggccg catttctggg ggcctctatt ggtcgttatg ccaaccgcat
    12061 cgccaatagc cgttatacct ttgacggtga aaccgtgacg ctttcaccaa gtcagggcgt
    12121 caaccaattg catggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca
    12181 gaatgatcgt caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gtttcccggg
    12241 taatctcggc gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta
    12301 tcgcgccaca gttgataaac cttgcccggt gaatatgact aatcacgtct atttcaatct
    12361 tgacggcgag cagtctgacg tgcgcaatca taagttgcag attctggcgg aagaatatct
    12421 gccggttgat gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacctcttt
    12481 tgatttccgc aacgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa
    12541 agtgaaaggt tacgatcacg cgttcttgtt acaggccaaa ggcgatggca agaaagtggc
    12601 ggcgcatgtc tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctccggc
    12661 tctgcaattc tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta
    12721 cgccgactgg caagggctgg ctctggaaag cgaatttctg ccggacagcc cgaaccaccc
    12781 tgaatggccg caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga
    12841 atatcagttt attgctcagt aatgttaagc cctcgtcatg agggctttat cttcatattg
    12901 ttcaaatcac cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact
    12961 gcgacactat tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat
    13021 aatgagaatt attatcatta aaagatgatt tgaggagtaa gtatatggct gtaactaagc
    13081 tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt
    13141 acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga
    13201 aagaggaagg ttacagcttt gactttgctt acacctctgt gctgaaacgc gctattcata
    13261 ccctatggaa tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga
    13321 aactgaacga acgtcactac ggtgcattgc agggtctgaa caaagcggaa accgctgaaa
    13381 agtatggcga cgagcaggtg aaacagtggc gtcgtggttt tgcggtgact ccgccggaac
    13441 tgactaaaga tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa ctgagcgaga
    13501 aagaactgcc gctgacggaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga
    13561 atgaaaccat tctgccgcgt atgaagagcg gtgagcgcgt catcatcgcc gcacacggta
    13621 actcgttacg tgcgctggtg aaatatctcg ataacatgag cgaagaagag attcttgagc
    13681 tgaatatccc gaccggcgtg ccgctggttt atgagtttga cgagaacttc aaaccgctga
    13741 aacgctatta tctgggtaat gctgacgaga tcgcggcgaa agcagcggcg gttgctaacc
    13801 agggtaaagc gaagtaaacg tcattcgttt aaaaatgaga aagccgaccg caaaatgagt
    13861 cggctttttt atttttcgtt gctaccaatg gagcacatcg cctgatgcga cgctgcgcgt
    13921 tttatcaggc ctccggtgat taatcggata cgccactctg acggcgcatc cgacaattaa
    13981 actttacccg cgacgcgctt ttactgcatt cgccagttga cgtaacagag catcggtatc
    14041 atcccagcca atgcaggcgt cggtgatgct cttaccgtag gccagcggtt ccccgctctc
    14101 gaggctctga ttgccttcca ccagatggct ttccaccatc acgccaataa tggccttttc
    14161 gccaccggca atctgctggc aaacgtcagt acaaacatcc atctgctttt tgaattgttt
    14221 tgacgagtta gcatggctga aatcgatcat cacctgtgct ggcaggcctg ctttgttcag
    14281 cccttctttc acttcagcaa cgtgcttcgc gctgtagtta ggctctttac caccgcgcag
    14341 aatgatatgg caatcgccgt taccgctggt attcacaatc gccgaatgac cccatttggt
    14401 cactgaaagg aagcagtgcg gcgcaccggc ggcattaatg gcatcgatag ccactttaat
    14461 cgtaccatca gtgccatttt tgaaacctac cggacaagaa agaccagacg ccagttcgcg
    14521 gtgcacctgc gattcggtgg tacgtgcacc aattgcgccc cagctcatca ggtcagcgag
    14581 atattgtggg gtgatcatat ccaggaattc acccgccgct ggcagaccgc tgtcgttaat
    14641 atcaagcagc aatttacggg caatacgcag accgtcgttg atctggaagc tgttatccat
    14701 atgcggatcg ttaatcagcc ctttccagcc caccgtggta cgcggctttt caaaatagac
    14761 gcgcattacg atttccagct catctttcag ctcttcacgc agcgccagca agcgagcggc
    14821 atactcttta gccgcgacag gatcatgaat tgagcatggg ccaatcacca ccaacaggcg
    14881 atcatcatta cctttcagga ttttatggat cgcttttcgg gcatgggcga ccgtattcgc
    14941 ggcattttca gtagcgggga atttttccag caatgcgaca ggaggaagta actctttgat
    15001 ttctttgatg cgtaaatcgt cgttctgata attcatgtct gttccagtgt tgccatactt
    15061 atcttagtga atgcaatact tccaatctat atcttccgtc ataatgtgta aacggggttt
    15121 tacactatga acggattaat cctggaatta gcaagaaaaa cgccagattg tcgcgaaaaa
    15181 cgagatctct cctacaattt ctaactgtaa ctcctttgtt tgttaattat ttcaagattc
    15241 tctgctgggt atctttgcct ggctgaaatc ttaaaccatt gccttatatt cacctgcaaa
    15301 tgcactgttg gaagaggtta tccgacataa cgaccataac aggagcatcc tatgaaaatg
    15361 acaaaactgg ccacactatt tttgactgcc actctaagcc ttgccagcgg tgccgcactg
    15421 gctgccgata gcggagcgca aagcaataac ggccaggcaa acgccgcagc tgatgcgggc
    15481 caggtagccc ctgacgcccg tgaaaatgtc gcgccaaata acatagacaa taacggggta
    15541 aataccggtt ccgacggcac aatgctgcat ccggatggtt cttcaatgaa caatgacggc
    15601 atgaccaaag atgaagagca caagaacacc atgtgtaaag atggtcgctg cccggatatc
    15661 aataaaaagg tacaaactgg tgatggcatc aataatgatg tcgataccaa aaccgacggt
    15721 accacgcagt aaccctgcaa aatagccaat aatgggagag cggagtctct cccttttaat
    15781 ttttcccttc tacaagcagt atgatgtcat aaagttaagt gagaataatt aagggatgac
    15841 tcgtcatggc gcactcacac tcacacgctt cctcacacct gccagaagat aataatgctc
    15901 gtcgcttgtt gtatgctttc ggcgtgactg ccgggtttat gctggtagaa gtcattggcg
    15961 gttttctttc cggttctctg gcattgctgg ccgatgcggg tcatatgttg accgatactg
    16021 ccgccctgct tttcgcccta ctcgccgtgc aattttcacg tcgacctccc accattcgcc
    16081 acactttcgg ttggctaaga ttgaccacgc tcgcagcatt tgtgaatgct atcgccctgg
    16141 tggtgattac cattttgatt gtctgggagg cgatagaacg cttccgcaca ccgcgtccgg
    16201 tcgagggcgg catgatgatg gcaattgccg tggctgggct gttggcaaat atactttctt
    16261 tctggttact tcatcacggt agcgaagaga aaaacctcaa cgtgcgagca gcggcactgc
    16321 atgtgctcgg ggacctgctg ggttcggttg gggctattat tgccgcgttg attattatct
    16381 ggaccggctg gacacctgct gaccccattc tctcaatact ggtgtcgctt ctggttctgc
    16441 gtagcgcgtg gcgactgttg aaagatagcg tgaatgaatt acttgaaggt gcaccggtat
    16501 cgctggatat cgccgaactg aagcgtcgta tgtgccggga aatcccggaa gtccgcaatg
    16561 tgcaccatgt acatgtatgg atggtaggcg agaagccggt gatgacgctg catgtgcagg
    16621 tgatcccgcc acacgatcac gatgccttgt tagatcagat ccaacactac ctgatggatc
    16681 actatcagat tgagcacgcc actattcaga tggaatatca accttgtcat gggccagact
    16741 gccacctgaa tgaaggggtg tctggccatt cacatcatca ccattaatgg gacagcgcgc
    16801 gtgaacctct ttcgcgtgcg ctgttgatcc acatccggct gccgttgagg gcaataaagg
    16861 tcagaatgat gtactccaga gacatggcgt aaacgccctg aagtgcaaaa ataacgacgc
    16921 taatcacgtt aataatcacc cacaacagcc agttttccac atacttacgc gtcatcagaa
    16981 tcattgccac gatagataac accatcatgc atgaatccca gaacgggaaa gcgtccggct
    17041 gtagttcagg catcgcaacc tgtaatccta atgcttgcat gatcatgact gccacgcggg
    17101 tcaaaaatgc aaacaccgga ttgataaata ccgtcatcag accaatcgaa acaacgcaaa
    17161 ccgccaacca gcttagcgcc ttcggcaatg gcaaccagcg aattttcaac tccgcctcgt
    17221 tctgactggt ttgtcgcgac cacgcatacc agccgtaaat attcgcggca aagaaaaaca
    17281 cctgtaataa caggctggca tacagctgaa tctgaaagaa aataatgcca aacaaggtga
    17341 cgttgatcag gccaaagaag tagttactga tcttctccag actggcaagg ccaatacaca
    17401 gcaacccggc gatcgtgcct accgcttcga tccatgagag atcataaccg cctgccccta
    17461 ttggtatatg taccaggata ttctgcacac taaaaaaatc catcgttccc ccaaagcgta
    17521 atgtgacgcc ttattatcaa ttatccacgt agtgtagccg caaaatccag catacgattg
    17581 agcggcacca gcgccctctc tcgcagcctt tcatcaacat aaacctcgtg attgcttcct
    17641 tccagttcta atgcctctgc gatggcctga aggccattca tggccatcca cgggcaatgc
    17701 gcgcagctgc gacaggttgc gccctcaccc gcggttggtg cttccagtaa ctctttatct
    17761 ggcaccgcct gctgcatttt gtagaaaatg ccccgatcgg ttgccacgat aagccgctga
    17821 tgtggcaatg ttttcgcagc agcgatcagt tgactggtgg aaccgaccgc atccgccatc
    17881 tctacaatag cttgcggtga ttctggatgc accagtatgg cagcatccgg gtattcttct
    17941 tgcaagcggg ttaacgcttg agtcttaaat tcatcatgca caatgcaggc accctgccag
    18001 cataaaatgt ctgcgcccgt ctgtttttgc acgtaacgcc ccagatgttt gtcaggtgcc
    18061 cagatgattt tttcacccaa actatccaga tgatcaataa gttcgacggc aatgcttgaa
    18121 gttaccaccc aatctgcgcg cgcttttacc gcagcagaag tgttggcgta gacgacgaca
    18181 gtacgatcgg gatgggcatc gcaaaatgcg ttaaattctt caacagggca gccgagatcc
    18241 agtgaacatt cagcctgaag tgtcggcatc agaattgttt tttccggact gagaattttg
    18301 gcggtttctc ccataaatct caccccagca actaacaacg tagaagcggg atgctttgca
    18361 ccgaagcgcg ccatttccag agaatcagaa atacagccac cggtttcttc tgccagttgt
    18421 tgaatttcgg gatcggtata gtagtgggca accatcaccg cattacgttc ttttagtaga
    18481 cgttttatct tctcgcggta atacgctttt tcatcaatgc ttaacggcgt cggcttcggg
    18541 gggaaaggat aaatcgccgt gtctggatca aacatcacgc tcatcttact atctcgtttt
    18601 acaggcttaa cgttaaaacc gatttaagcg tgatattcgc tattatcggc gtaatttgtt
    18661 ttatatgcta aacaagatag tcgaatggtg gagaaaagtc actcgaatta cgtatgagat
    18721 gccgaatgcc acactttgcg tcttattcct ccgacgtaga gttgtttacg cagcaaacgc
    18781 ggagtatgtc atcagggtca agaattgaaa ttgagagatg cctgaatagc aaaaaagcgc
    18841 ctttagggcg cttttttgca ttggtgggtc gtgcaggatt cgaacctgcg accaattgat
    18901 t
//
LOCUS       DAZFRG010000083        18874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFRG010000083 DAZFRG010000000
VERSION     DAZFRG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.083"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1053
                     /locus_tag="ACHKUC_002241"
     CDS             <1..1053
                     /locus_tag="ACHKUC_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6618495.1"
                     /translation="AIKLKGLFYYPSSVYQSCLHAAEQGYSLAWYSLGKMYYEGDEVS
                     QDLKLAFNWFTRAAQHNVIDALYALGIMYSDGRGTDKNICEARKWFLLAAQNGNTSAQ
                     YELARISRFAVEPLRNYEEALQWYLSAATQGHEVAQYELGQMYIQGIGVERDEVQAHR
                     WFLQSAEQGYLHAQYHTARLYSGSESISQDQEKALYWFTKAAKNGADGAGDAMYELGK
                     YYLTNNDDPENNAEATQWLTGAAQRGRIEAIFLLAEMYLYGTKDTIKDENHALHWYEK
                     AARLGSTEAQHQTAAMYAQGTGTKIDNKQAWMWLTIAGNNNPWIEKDALRCLMSEQDI
                     QDAEQTAANCKRLLRI"
     gene            complement(1109..2452)
                     /gene="gdhA"
                     /locus_tag="ACHKUC_002242"
     CDS             complement(1109..2452)
                     /gene="gdhA"
                     /locus_tag="ACHKUC_002242"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO6618496.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            2688..2960
                     /locus_tag="ACHKUC_002243"
     CDS             2688..2960
                     /locus_tag="ACHKUC_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO6618497.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2926..3333)
                     /gene="nudG"
                     /locus_tag="ACHKUC_002244"
     CDS             complement(2926..3333)
                     /gene="nudG"
                     /locus_tag="ACHKUC_002244"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO6618498.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            3420..4040
                     /locus_tag="ACHKUC_002245"
     CDS             3420..4040
                     /locus_tag="ACHKUC_002245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6618499.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(4049..5356)
                     /gene="ynjE"
                     /locus_tag="ACHKUC_002246"
     CDS             complement(4049..5356)
                     /gene="ynjE"
                     /locus_tag="ACHKUC_002246"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO6618500.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFIYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5423..6076)
                     /locus_tag="ACHKUC_002247"
     CDS             complement(5423..6076)
                     /locus_tag="ACHKUC_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6618501.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGKNLLLALP
                     ATLNGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVTLRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(6076..7611)
                     /locus_tag="ACHKUC_002248"
     CDS             complement(6076..7611)
                     /locus_tag="ACHKUC_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO6618502.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7584..8741)
                     /locus_tag="ACHKUC_002249"
     CDS             complement(7584..8741)
                     /locus_tag="ACHKUC_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6618503.1"
                     /translation="MRLCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDATFA
                     RVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKIASG
                     DLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAVWGD
                     PSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(8751..9299)
                     /locus_tag="ACHKUC_002250"
     CDS             complement(8751..9299)
                     /locus_tag="ACHKUC_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6618504.1"
                     /translation="MGLPPLSKIPFILRPQTWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9299..10006)
                     /locus_tag="ACHKUC_002251"
     CDS             complement(9299..10006)
                     /locus_tag="ACHKUC_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6618505.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(10020..10697)
                     /gene="ydjY"
                     /locus_tag="ACHKUC_002252"
     CDS             complement(10020..10697)
                     /gene="ydjY"
                     /locus_tag="ACHKUC_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO6618506.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10702..11412)
                     /locus_tag="ACHKUC_002253"
     CDS             complement(10702..11412)
                     /locus_tag="ACHKUC_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6618507.1"
                     /translation="MNAERKFLLACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(11579..12385)
                     /gene="xthA"
                     /locus_tag="ACHKUC_002254"
     CDS             complement(11579..12385)
                     /gene="xthA"
                     /locus_tag="ACHKUC_002254"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO6618508.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12831..14051
                     /gene="astC"
                     /locus_tag="ACHKUC_002255"
     CDS             12831..14051
                     /gene="astC"
                     /locus_tag="ACHKUC_002255"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO6618509.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYACMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGSNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            14048..15082
                     /gene="astA"
                     /locus_tag="ACHKUC_002256"
     CDS             14048..15082
                     /gene="astA"
                     /locus_tag="ACHKUC_002256"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO6618510.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGEFPACLVANENYHHFRVVLVRTDPATGRLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            15079..16557
                     /gene="astD"
                     /locus_tag="ACHKUC_002257"
     CDS             15079..16557
                     /gene="astD"
                     /locus_tag="ACHKUC_002257"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO6618511.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRATGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAGYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16554..17897
                     /gene="astB"
                     /locus_tag="ACHKUC_002258"
     CDS             16554..17897
                     /gene="astB"
                     /locus_tag="ACHKUC_002258"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO6618512.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFRGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPHVALLGDEGAANHNRLGGHYGEPGMQIFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNHVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSAYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            17890..18858
                     /gene="astE"
                     /locus_tag="ACHKUC_002259"
     CDS             17890..18858
                     /gene="astE"
                     /locus_tag="ACHKUC_002259"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO6618513.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4476 a         4835 c         5121 g         4442 t
ORIGIN      
        1 gccataaaat taaaaggtct tttttattat ccatcttctg tataccagtc ctgcttacat
       61 gctgcagagc aaggctattc gctagcctgg tattctcttg gcaaaatgta ttacgaaggt
      121 gatgaggttt cacaggatct aaaattagcc tttaactggt ttacccgcgc cgcgcagcat
      181 aatgttatcg atgctctgta tgcgctgggt atcatgtata gcgatggaag gggcacggat
      241 aagaatattt gtgaggcacg aaaatggttt ttacttgctg cccaaaatgg caacacatct
      301 gcgcaatatg agcttgcccg tatctcacgc tttgctgttg agccattacg aaattatgaa
      361 gaagcattgc agtggtattt atctgccgcc acacaaggac atgaggttgc ccagtatgag
      421 ttgggacaaa tgtatataca agggattggc gtggagcgcg atgaggttca ggcccatcgt
      481 tggtttttgc aatcagcaga acagggatat ttacatgcgc aatatcatac tgccaggttg
      541 tatagcggaa gtgagagtat ttcacaggac caggaaaaag cgctgtactg gtttaccaaa
      601 gcagcaaaaa atggggcaga tggggcaggg gatgccatgt atgaattggg gaaatattat
      661 ttaaccaaca atgatgatcc tgaaaataac gcagaggcta ctcaatggct tactggtgca
      721 gcgcagcgtg gtcgaataga ggcgatattt ttgttagctg aaatgtatct gtacggtaca
      781 aaagatacca taaaagatga aaatcatgcg ctgcactggt acgaaaaagc ggcgcgttta
      841 gggtcgaccg aggcgcagca ccagactgcg gcaatgtatg ctcaggggac gggcactaaa
      901 attgataata aacaagcatg gatgtggcta acaattgccg gaaataacaa cccctggatt
      961 gaaaaagatg cgttgcgatg cttaatgtct gaacaggata ttcaggatgc agaacaaacg
     1021 gctgcaaatt gcaaacgtct gctaaggatt taattaactc agggaaaatc gtctggttat
     1081 aaggaaaacc agacgattgt aataacgatt aaatcacgcc ctggcttaac atcgcatccg
     1141 caaccttcac aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac
     1201 caccatgctc aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa
     1261 ctttctcggc tttccagccc aggcgcgcag cgttttgcgc catttccagg cccgatgttg
     1321 ctacaccgcc agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca
     1381 gttcagtcgc ttcgatggtg gtcggcatat ttgccccttc ggcgacggct ttaacgccat
     1441 tggcgataag ctgatgcgcg gcgtcaacat ccagttcgtt ctgggtggcg caaggcaggg
     1501 cgatatcaac cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt
     1561 tggcgtaatc tgccactcga ccatcgcggc tggctttgat ttcgataaga cgcgccagtt
     1621 tttctttcgt gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca
     1681 ctcgagcacc aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag
     1741 aaacggaaac gcgcattcct tcaaaaccca taccgtggcg ttttagcatt gcttctgtga
     1801 aataaaccag accgtagccg gtagcttccg ggcgaataag actgccgcca aatgaaaggc
     1861 ccttaccggt gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa
     1921 agccgacttc acgaccacca acgccgatat cacctgccgg aacgtcagta tccgcgccca
     1981 ggtggcgata cagttcagtc atcagcgcct ggcagaaacg cataacttcg ccttcgcttt
     2041 ttcctttcgg gtcgaaatcg ctgccgcctt taccaccgcc catcggtaga gtagtcaggg
     2101 catttttgaa ggtttgttcg aagccgagga atttgagaat ggagaggtta actgacggat
     2161 ggaagcgcat accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac
     2221 ggttgacctg cacctggttg cgatcatcaa cccatactac gcgaaattgg atcacgcgct
     2281 ccggttcaac cagacgctcc agtaatgaca tctgacgata tttgggattt tgttcaagaa
     2341 aaggccagag tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt
     2401 cgcgcttttg gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa
     2461 aacccttata tattaatacg attatgctta tgttgtttat gtcatgtgct tttgcagttt
     2521 tcgactatac catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat
     2581 taataacgag agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccgcc
     2641 cggctaccgt tatctgtaga agagacaata acgttaagga gttccctgtg agtcgagcat
     2701 tgatcgccgt agtattagca tttccattaa ttacactggc taacccgcat tatcatccgg
     2761 acgtggaggt aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct
     2821 gtactcagtg ctgcgtttat caggatcaaa attattctga aggggcagta attaaagccg
     2881 aaggaatact gctgcaatgc caacgggatg acaaaaccct aagcactaat ccgctggtct
     2941 ggcggcgcgt gaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata
     3001 tcgcagcgcc tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt
     3061 cccgtggaag tcgggtacgt gccaggcatg aagatggata atccgccccg aaacttctcg
     3121 ctgatggctg gcaacatagt caccaacagt tgcttcgatg cccagttctt cgtttaactc
     3181 acgcaccagc gcctgccgct ggctttcatc cggctcgact ttaccaccgg caaactccca
     3241 taatcccgcc tgatcgctat gggcagggcg ttgcgcgagt aaaattttgc catcacgttc
     3301 gatgatggcg gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa
     3361 aatgccataa tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg
     3421 tgctagaccg ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc
     3481 ttgataaacc gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc
     3541 tggcactgcc ttttctggcg ctgggctggt atctggcggc actagtcgcc attttgttga
     3601 acaggctgct tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg
     3661 gcggctttct cgatatttct ctcgattttc ttttttacgc gctggtgcct tttgggttta
     3721 tcctcgccgc accggagcaa aacgccctgg taggcggctg gctgctgttt gcgtttatcg
     3781 gcacgggcag cagttttctc gcttttgctg cgctggcggc gaaacatcag attgataatc
     3841 ctggttatgc gcataaatcg ttttattatc tgggcgggtt aaccgaaggc agcgagacga
     3901 ttttgctgtt tgttctgagt tgcctctttc cggcctgttt tccgtggttt gcgtgggtat
     3961 ttggtgcgtt gtgctggatg acgaccttta cgcgggtgtg gagtgggtat ctgacgctga
     4021 agtcgctcca gcgtcagtaa ttgcaatgtt atttactgct gtccgggccg cgttcaccgg
     4081 ttgctaccgg attttttgga tcgctgctcc attcgtacca gccaccgtcg taaacggaga
     4141 cgttattcca gcccattgcg cgtgcgtaaa taaaggtttc tgacgcgcgc cagccagtgc
     4201 cgcagtagaa tgaaacttgc tgatctggtt tgatattcca tgctttccac atagcggtta
     4261 tatcatcggc gctgcgcatg gtgccatccg ggttatggaa atcttccata tgcgtcgagt
     4321 cgctacctgc gtgtccccaa cgtgctccgg ctatttcacc ttttggttta atatagctgt
     4381 aaccgctggt cgtaccgata aattctggcc acgaacgaat gctcaccagc gatgcatcct
     4441 ggcgatgcag cagtccacgc gcttgttcca tatcaagcat cagttgcggt tgtgccggga
     4501 tcttcacgcc gaaatccggt tccgctttca ctttcggtgg cgttccgcgc tcaacgggca
     4561 gacccgcgtc ggaccaggtt tgccagcctc cgtccagcag gcgcacatct ttcacgccgg
     4621 cataaagcat aatttgcgcc acacgcgctg cagcgtatac gtcacgcccg tacagaatga
     4681 ccgtggtgtc atggcgaata ccgtgttttg ccagcatcgc tttcagttgt tcatcagaaa
     4741 ctttgttcca cagcggttcg ctttccactt cgttggtgtc gatgtagtca gcgccgggaa
     4801 tatggctgat aaggtaaaat tttggagcgc cccaggccgc ttcaatgact ttccagtcac
     4861 cggcaggttt cgccgtaacc tctttacctt gttgcaggtc atgcagccac tgcggatata
     4921 ccagctgttc aaagtgcggc agtttttgca gacgggaagg ttcgcttagc gcgtcactca
     4981 ggatggagat atgcgtaaaa cctgcttttt gcagtcgcgt tttgacggcg tcgacatctt
     5041 tgtcattacc gtacagcgcc accggggcat cggctttcag gttatgttgc ttgatccact
     5101 cgttgagctg ttcggtgctc attttgtcga gccagctagc agagaggttt aaggcggcag
     5161 gttcatgacc ggaagggcca tttaaggttt gtggccagcc gttataaaac gcgctggggc
     5221 gagtatcaat cgctttgcca ttttgttgtt gaagctggtc aagtgtaaga ggcttcgcca
     5281 gttcagcggc ccacgaagaa gcgcaagcca gccctaaagc cattgccagc gcggtcattt
     5341 gagaaacacg tttcatcgaa aatcccgaca ttgtagtttt gagagcgcca ttctgaactg
     5401 acgcatcggg aaaaactttg cgttatcgca gtttgttgta attttctgac cactgcgcca
     5461 tatccagaac ggggctatca ggaggaacat cctgaagatc gtgcgtcacc tgcacaacag
     5521 ggatcgccat ggcgcgaatt tcgctgaaca cccaatggcg aaaattatcg cgcagagtca
     5581 catcaagacg gctgaatggc tcatccagta gcaacgcttt tggctgagcg agaagggcgc
     5641 gaagcagagc aacgcgcgct cgctgaccgc cagacaaagt ggcaggatcc tgatggaacg
     5701 ctccgtcaag gcctgaacgc tcaagtgcat cattcacggc attacgtcgg gcattcccgt
     5761 taagtgtcgc cggtagcgcc agcagtaaat ttttcccgac gctgaactgg tcaaataaca
     5821 gtgcatcctg gaaaagaatg ccaatctgac gctgtgcggt gggtagcatg tcaatccgtt
     5881 gctcattaag ccatagctcc cctgtacaag aaaactgtcc agccagcgca ccaatcatcc
     5941 acgaaaacag agtggatttc ccgcagccgg atggtcccat taacgtgaca atgtcacctt
     6001 tatctactgt aaagttaacg tttgtcagca agcggctttc tggtaaacgt agcgaaacat
     6061 ttttcacgca gagcattagc ggagtccttg tctgacataa cctacccatt ttgcgactaa
     6121 cgcggtcagg gcaaaaataa taagcggtaa cagcagttgc cataaagcct gggcggcgag
     6181 aataccgttg ctgccgccgc tgcttaatgc caccgcttca gtggtgagcg tcggaaaacg
     6241 ccccgcgccc agccacagcg tcggcatata ctgcgcaata ctgactgaaa atcccaccgc
     6301 gaaggcaatc agcgcagggc gcaacatgag tgggcatttt acgtaaaaga agattttggc
     6361 ccgcgaccag cccagtgttt gcgcaatcaa aattaaccgt gaatcaatgc gctgccaggc
     6421 gggttgcagg ataaacagca tccacggcat tacccatagc agatgccccc agaccaccgc
     6481 cgtccagctt ccatccaggt tcagccatag cgccagcgtg tactggcccg ccaccagcgg
     6541 cagagcgggt aataaaatgg gcaaccatag ccacaactgg cgacgctgtg gcccccattc
     6601 cagccacagc agtaacagga gcaaagcgat gaatgccgcc accagcccca ttgtcaggct
     6661 gttgatgagc gcttcactat tgatcgtcga ctgatccgcg aggatcgcca gcagaaccac
     6721 acacagcaca ccggttaagg gtaaaaaact cgccagcgta ttgcctggca ataaaggcgt
     6781 ggcaggctta cgaacgccat ctacgcgggg aatagtacgc cgccagccac gccatagcag
     6841 atagcccagc aaaacgtagg cggcgagtaa cagcatcagc agcaggctgg cgagtgcacc
     6901 tttcgtttgt tgatcggcgt cgccctgagt taaccactgc cagctaatta ccgccagcgt
     6961 cgggggatta cctggcccaa gaataatcgc cacatccacg accgacagcg accaggcgac
     7021 aatcgccagc atcgccattg ccagcgcaga cgcgacggag ggcaacagca accagttcag
     7081 gcattgccag cggctgtagc ccagcgaatc cagcacaatg agttgctgca acagccgttt
     7141 ttcgctcaat actgccgcta agatccacag cagaaatgcg ctttctttca ccgccagggt
     7201 gaggcccagc ccaataccca atttgtccat tggcggagtg aaatacggga aatagtcata
     7261 aagcagccct ccgtcagcaa agagcagaag ggcgctggtg gcaaaagcca catggggaat
     7321 ggcgagcagc cacggcagac gggcgcacat acgctgccat ttcggccctg gccacaacgc
     7381 cacaatcacc agcagggcaa tcaacaacgc cccgaccgcc gcaatggttg tcgacaccag
     7441 cgtcgccagt aatgcctgcg gtaactgtgg atcggcaaat aacgcctgcc agtgcgtcaa
     7501 cgacaaagca ggcgatatca gcgtgagagc tgccgggatc agcggtgcat aaatcaccgc
     7561 tatcatcgcc cacagcaaaa agattaatgc gtaccgtaac ggtgtagcca ttcttgttcc
     7621 agcgcattta cccaacctgc gtgcggttca gccagtaccg gcggcagatc ctgcggcatt
     7681 ctcgattgca atgtttcgcg ctgcccgtca ggcagttttt gcggatcgag aacagaagga
     7741 tcgccccaga cagcgggatc tgctttacgc agttgcgcat cgggtgagag caggaaattg
     7801 gcaactacct tcgccgcagc actggcattc gcgttggcag gaatggtgac gaaatggacg
     7861 ttgccaatca tcccctcgcg aaaaccaaaa ctgtaactgc ttgcaggcaa atcgccgctg
     7921 gcgatttttt gctgcgcatg tgcagggtta aaggtcagcg acaggcgcaa tgtgttagct
     7981 ttcagcagag catccatccg cgcgggcgaa ggcgggaaat cctttccttc gcgccataaa
     8041 tacggatgca gtgcatcaag atattgccac aagggcgcag tgacgcgggc gaaagtcgca
     8101 tcgtccggcg cttcttttaa tgctgtggga tctggcgtta gcataatcag caactgttca
     8161 agaaacgccg tgccggtaaa gtccggtggg cggggatagg taaccgtgcc gggattggct
     8221 ttagcaaact ccagtaaggc ttgcggcgtt tgtggcgtaa catcgcggcg ggcgataaac
     8281 gtcagttgtg cgccgcccca gggcgattca gccccttctg taggcactga aaaatcttcc
     8341 cgcaccggaa gctgtgtgtc gacatagcgc cagttgggca gagtctccgc ccagcccgtt
     8401 tgtaataaat tggcctcttt taaggtgcgg aagttttcgc cgttcaccca gagcagatcc
     8461 accgaaccgc ccgttttacg tccggcagca acttcggtct gaatgcgttt caccgcgtct
     8521 gcggcatccg cgagacgaac aatcttcagg tttatagcgt aatgggtttt catctcgccg
     8581 ctaacccagt cgagatagcg gttaattgcg gtatcgccgc cccaggcgtt aaaccagacg
     8641 gtttgtcctt tggcctcatt ttttatttcc tgccagtcac tggcatgtgt tgccaaagaa
     8701 aacagcgata acaatcccag caaccaccca caaaggcgca tcttctctcc ttaagaacgt
     8761 ttttcaggaa tacggcacag cccctgagcg ggaatgtcca tggcagaatt aaaacgggct
     8821 gacagatttt gcaggcctat caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc
     8881 gtagccagtc gggcgcgcag ggcatcatcg accgttggcg gcgttacgct tgcggcttcg
     8941 gcgtactcca gcgccagccg ttcttcatcg ctaaagagcg ggctttgccg ccagtcagcc
     9001 actgccagca gtttatcggt gctgccggta cgctcggcga ctttcatact ggtgatatcc
     9061 acgcaaaact cacacaggca catttgcgca atgcgcgcgc tgacaagcga tcgtactacc
     9121 ggatcgagcg gtgagcgttt gcgctccagc cagccgacaa acatcgacac cagataaaag
     9181 ataaacggga tccgccccca ccagcgaatc gggcttagca cctcgccgta atggcgacga
     9241 tgcagccacg tctgcggacg taaaatgaaa ggaattttgc taagcggcgg taaacccatc
     9301 aggcattcct cttctggcgc tcaagccata ttttcttcgc cataaaaatc accacggtca
     9361 gggcaaacag aataaacagt ccggtgacaa accagaaagt accgcctgtt aacatgctgc
     9421 ccgcccagga ataaacaata gtcgccggta attgaccaag cccggtggcg ataaaaaacg
     9481 agcgaaaacg tattgaagtc aaaccggcag catagctgat tggatcgaaa gggacaaaag
     9541 gcaataaccg acataccagg atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac
     9601 tgtcaagtac ggttttgccg gttaattttt ccaccacttc gcggcccatc actctggcga
     9661 taaaaaagca cagtgccgcg ccggccatcg aactggtcca cgacagtaag ccgccccaga
     9721 acgcaccaaa cagcgacgca ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa
     9781 tggcctgtaa aatcatcaac aagaacgaga caactgccgc tagtgcgccg taagactgaa
     9841 taaagcgttc tataccctgt tggtccacgg cggcaaacgc cgcaacgctg cgattgataa
     9901 actcatggac gccgggaagc agcgcccatg ccagcagcac agcgaacaac aggatgatga
     9961 gggttatacg gtagtaccag attttacgcg attgcatctt catcatccgt tatcctgctt
    10021 tattcggtga ttttaaaggt aaccgttgcc agcgtgttat ccgccgggag aactgaggca
    10081 ttgcctttga atttcacttc gccgcgtttt tccaccgcac cataagtgta tgttgcattg
    10141 ctgacgatgc cgaccgggca gctatccaga cacaccaggc aacccgtttt cttctcttct
    10201 gctgccgtta aattaccgcc aaagcgcatg tccagtttct tgccattact gtcaacaatc
    10261 acttcatcga aggaatacgc ttttgccgcc ccttgccagt tgaccgaaat atccagtttg
    10321 ctgcctgtga catgagtcgt ttctttatta tccatcgtca tgttttcgcc cggcgtacca
    10381 cctgcctctt tcagggcttc ataaaatgct ttcggggtcg cataacccat aaacagcgat
    10441 ttatgtccgt tggagccatc tttaaacaca ataccgtgac gagtgtcgtc ggtgaggaaa
    10501 cgaccattaa tttgcaccag catggttacc gtatggttat tggcatcgac acgcagcggt
    10561 tggctgttcg aaagtccgtc gtattccact tttgcagccg cattctcttt ttgatcgcag
    10621 ccgctaagcc cagcaacagc cagtaagcag agtgcagcca gtcctttttt ccatgacact
    10681 gaataatgtt gcaacatcgt tctatccttc attttttggg tgagaaagtg ggctgcggcg
    10741 agaagcagac agatccacat gtttgtgtcg ggcgtagagt tttgcgagct ggacgagaat
    10801 aaacagcgcc aggcccgcca gacagagttg taaaataaag cgcagcgtaa tgccttcatt
    10861 ggcgagatcg cttgccatca cggtataaat aacaataccg ggtagggtcg tgagtgccga
    10921 aataagggta taaggccaga aggcgattgt ggttaatccg taagcgtaat tttgaatatt
    10981 gtaaggaaac aacggtatta agcgggtcag aataagaaaa tcaataccgt tacgcgcaat
    11041 gcctttttca atggcctgaa aggtatggct atgaccaacg tatttcagca gtaaatcacg
    11101 tcccagccag cgcgccagca ggaatgagca cgaggaggcc agcgtggcgg caattaatga
    11161 tagtagtgtc cctaagagcg ggccaaaaac tattcctccc gcgatcacca gtatgcttcc
    11221 tggtaacaga aagagggtgg caatgatgaa taacagaata tagaggctat agccgaacaa
    11281 tccgctctgg cgaatgagtg tctgtaagtg gggtaaatcg gtgagcagat cgaataaacc
    11341 gaaagcgtgg atagcgtaaa tgaccagcgc aaaaataaga caggctaaaa gaaatttacg
    11401 ctcagcgttc atcgtggagt ttcctatatc aaacttgata atagagaagt ggtcagacaa
    11461 ctaaaaacgc agtgacggta taacaaagga cggcaggcaa caaatcatta ctgacagttt
    11521 gttgattttt ctcaaggtta attctcctga cccagtttga gccaggagat cagctaaatt
    11581 agcggcggaa ggtcgcccag acgggggcgt gatcggacgg tttttccatg ctgcgaattt
    11641 catagtcgat gccggtttct acgcaacatt ctgccagcgg ttggctggcg agcagcaggt
    11701 cgatgcgcag accgcgatta tcgtcaaaac cttttgagcg gtaatcaaac catgagaaac
    11761 gatctgctgt ttgcggattc gcatggcgga aggtatcgac caacccccag ctcatcagcc
    11821 tctccatcca ttcgcgttcc tccggaagga aagaacattt tccggtacgc agccagcgct
    11881 tacggttctc ttcgccaatg ccaatatcca ggtcgccagg actgatattc atatcgccca
    11941 taatcagcac cggattttca cgcttgagtt cggtttccag gtagttttgc agattctgat
    12001 aaaactgcgc ctttgccggg aattttatcg gatggtcgcg gctttcaccc tgcgggaagt
    12061 aaccgttgat cacggtgaca ttgcccagcg gcgaggggat ttccgccata ataatccgcc
    12121 gttgcgcttc ttcgtcgtca ccaggaaagc cgcgacgcac ggcaatcggc gtctctttgg
    12181 tcagcagcgc cacgccataa tggcctttct gcccgtgata aaacacgttg tagccgagct
    12241 tcgccacctc ttcgagcgga aacatatcgt catgaacttt tgtctcctgc aggccaatca
    12301 catccggttg gtgcttttcg acgatggctt caagctggtg aggtctggcg cgcaggccgt
    12361 tgatattaaa agagacaaat ttcatagtcg ctgccattta ttcagataaa gagtgcgtgg
    12421 atggtagcag aatttcgctt tgcttaccgc ctgttgttaa gtgatttgat cgatggttgt
    12481 taatgatgtc aacgatggcg caaaaaatgc ccgcttttgg tgcgcgttgc gtcagaatgg
    12541 cgcagtaatt tccagtaaat ttcgtcaatg atcacaattc cagaattata ttttacttct
    12601 gcataacatt gcgtttttta ttctttttat tcacccacat cgcaaacgta ttcactttat
    12661 atgcacttta aatgcatatg atgtggttat aactgcttga tttccagtta gcctccgccg
    12721 tttatgcact tttatcactg gctggcacga accctgcaat ctacatttac agcgcaaaca
    12781 ttacttatta ttaacatata aataacgaat tatttactgt agaggtcgct atgtctcagc
    12841 caattacgcg tgaaaacttt gatgaatgga tgatacctgt ttacgctccg gcaccgttta
    12901 taccggtacg tggcgaaggt tcacgcttgt gggatcagca ggggaaagag tatatcgact
    12961 tcgcgggtgg cattgcggtg aacgcgctgg gccatgcgca tccggaactg cgtgaggcgc
    13021 tgaacgaaca ggcgagtaag ttctggcata ccggcaacgg ttacaccaac gagccggtac
    13081 tgcgactggc gaaaaaattg atcgacgcca cgtttgccga tcgcgtcttc ttttgtaact
    13141 ccggtgcgga agccaacgaa gcggcactaa aactggcgcg taaattcgct cacgaccgct
    13201 acggcagcca taagagcggc atcgtggcgt tcaaaaatgc gtttcatggt cgcacgctgt
    13261 ttactgtcag tgcgggtggg cagccagcct attcacagga ttttgcgcca ctgccgccgg
    13321 atattcgtca tgctgcatat aacgatatta actctgccag tgcgctgatt gacgacgcca
    13381 cctgtgcggt gattgtcgaa cccatccagg gggaaggcgg tgtggtgcca gccagcaacg
    13441 catttttaca aggtctgcgt gaattgtgtg accgccacaa tgcgttgctg atctttgatg
    13501 aagtacaaac cggtgtcggg cgcaccgggg aactgtatgc ctgtatgcac tacggcgtga
    13561 cgcctgatct gttaactacc gccaaagcgc tgggcggcgg tttcccggtt ggtgcgttgt
    13621 tggcaaccga agagtgcgcc agcgtgatga ccgttggcac tcacggcacc acctatggcg
    13681 gcaatccgct ggcctcggcg gtggcaggca aagtgctgga tctcatcaac acgccagaga
    13741 tgcttaacgg ggttaaacag cgtcacgact ggtttgttga gcgtctaaat tccattaatc
    13801 accactacag tttgttcagt gaagttcgcg gcttaggtct tttgattggc tgtgtactga
    13861 atgccgatta cgccgggcaa gcgaaacaga tctctcagga agcggtgaaa gcaggcgtga
    13921 tggtgctgat tgcggggagc aacgtggtgc gctttgcgcc tgcgctcaat gtcagcgaag
    13981 aagaggtgac gaccggactg gatcgctttg cagctgcttg cgaacacttt gttagccgag
    14041 gttcatcatg atggtcattc gtcccgttga acgctcagac gttgcggcgc tgatgcagct
    14101 tgccagcaaa acgggcggcg gcctgacgtc gcttcccgcc aatgaagcca cgctttcggt
    14161 gcgtattgaa agggcaatca aaacctggca aggcgaactg cccaaaagtg agcagggtta
    14221 tgtgtttgta ctggaagata gtgagacagg cactgtggcg gggatttgcg ccattgaggt
    14281 ggcggttggg ctgaacgatc cctggtacaa ctatcgtgtc ggcacgctgg ttcacgcttc
    14341 gaaagagctg aatgtctata acgcattgcc gacgctgttt ctcagtaacg atcacaccgg
    14401 cagcagcgag ctgtgcacgc tgtttctcga cccggagtgg cgcaaagagg gcaacggcta
    14461 tttgctgtcg aaatcgcgct ttatgtttat ggcggctttt cgcgacaagt ttaatgacaa
    14521 agtggtcgcc gaaatgcgcg gggtgattga cgaacacggc tattcaccgt tctggcaaag
    14581 cctcggtaaa cgcttctttt cgatggattt tagccgcgct gattttctct gcggcaccgg
    14641 gcaaaaggca tttattgcag aactgatgcc gaaacatccg atctataccc actttttatc
    14701 ccaggaagcc caggacgtca tcggtcaggt acatccgcaa accgcgcctg cccgtgcggt
    14761 gctggagaaa gaaggttttc gctaccgtaa ctatatcgac atctttgacg gcgggccgac
    14821 acttgaatgt gatatcgacc gcgtgcgcgc catccgtaaa agtcggctgg tggaagtggc
    14881 tgaagggcag cctgcgcagg gcgaattccc ggcctgcctg gtcgccaatg aaaattatca
    14941 ccatttccgc gtggtgctgg tgcgtaccga tccagcgacc gggcgtttga ttttaaccgc
    15001 tgcacaactg gatgctctca aatgccacgc aggggatcgc gttcgtctgg tgcgcctgtg
    15061 cgcagaggag aaaacagcat gactttatgg atcaacggtg actgggttac gggccagggc
    15121 gcatcgcgtg tgaagcgtaa tccggtatcg ggcgaggtgt tatggcaggg caatgatgcc
    15181 gatgccgctc aggtcgggca ggcttgtcgg gctgcccgtg cggcgtttcc gcgctgggcg
    15241 cgtctctcct ttggcgatcg tcaggtaaga gttgaacgct ttgccggatt gctggaaagc
    15301 aataaagccg aattaaccgc gattattgcc agagaaaccg gtaagccgcg ctgggaagcg
    15361 gcaaccgaag tgacggcgat gatcaataaa atcgcgatat caattaaggc gtatcacgtt
    15421 cgtaccggtg agcagcgtag cgaaatgccg gacggcgcgg cgagcctgcg acatcgcccg
    15481 cacggcgtgc tggcggtgtt tgggccgtat aatttccctg gtcatttgcc gaacggacat
    15541 atcgttccgg cattgctggc aggtaacacc attatcttta aacccagcga actgacaccg
    15601 tggagtggcg acgcggtaat gcgtttatgg cagcaggctg gcttgccgcc gggcgtgctg
    15661 aacctggtgc agggcgggcg tgcaacgggt caggcgctga gtgcgctgga ggatctcgac
    15721 ggcttgctgt ttaccggcag cgccaatacc ggctaccagc tgcatcgcca gctttccggt
    15781 cagccggaga aaattctcgc ccttgagatg ggcggtaata acccgctaat tatcgatgag
    15841 gtggcggata tcgatgccgc tgtccatctg accattcagt cggcgtttgt cactgccggg
    15901 caacgctgca cctgcgcccg ccgtttattt ctgaaaagtg gaacgcaggg cgatgcattt
    15961 cttgctcgcc tggttgccgt cagccagcgg ttaacgccgg gcacttggga tgacgaaccg
    16021 cagccattta ttggtgggct gatttctgag caggccgcac agcaggtggt tactgcctgg
    16081 caggaactgg aggcgatggg cggacggacc ctgcttgcgc cgcgcttatt acaagcaggg
    16141 acatcgttgc tgacgccggg gatcattgaa atgacaggcg ttactggcgt gccggatgaa
    16201 gaagtgtttg ggccgttatt gcgcgtctgg cgttatgaca atttcgatga agcgattcga
    16261 atggcgaata acactcgctt cggcctctct tgcggtctgg tttcccccga gcgggaaaaa
    16321 ttcgatcaac tgttgctgga ggcacgggcg gggattgtta actggaacaa accgcttacc
    16381 ggtgctgcca gtaccgcgcc attcggcggc attggcgctt ccggcaatca tcgccccagc
    16441 gccgggtatg ccgcagatta ttgtgcctgg ccgatggcga gcctggagtc ggactcgtta
    16501 acgttgcccg caacgcttaa ccccgggctg gatttttccg atgaggtggt gcgatgaacg
    16561 cctgggaagt caattttgac gggctggtag ggctgacgca ccattacgcg ggcctgtcgt
    16621 ttggtaatga agcctctacc cgtcaccgtt ttcaggtgtc taacccgcga ctggcggcaa
    16681 aacagggctt actgaaaatg aaaaaacttg ccgatgcggg attcccccag gccgtgatcc
    16741 cgccgcacga gcgtccgttt attccggtgc tgcgtcagtt gggattcagg ggtagcgatg
    16801 agcaggtact ggaaaaagtt gcacgccagg caccgcactg gctttccagc gtcagttccg
    16861 cttcgccaat gtgggtagcc aatgcggcaa cgatcgcgcc atccgccgat acgctggatg
    16921 gcaaagtgca tctcactgtt gctaacctga acaataaatt tcaccgttcg ctggaagccc
    16981 ccgtcaccga atcgctgtta aaagcgattt ttaacgacga agagaaattt agcgtccatt
    17041 cggcgttgcc gcatgtggct ttgctcggtg acgaaggagc cgcgaatcat aatcgtctcg
    17101 gcggtcatta cggtgaaccg ggaatgcaga tttttgttta cgggcgagag gagggcaatg
    17161 atacccgacc gtcccgctat ccggcgcggc agactcgcga agccagcgag gcggtggcaa
    17221 ggctgaatca tgtgaatcca cagcaggtga ttttcgccca gcaaaacccc gacgttatcg
    17281 accagggcgt ttttcataat gatgtgattg ccgtgagtaa ccgccaggtg ctgttttgcc
    17341 atcaacaggc gttcgctcgc cagtcacagt tactggcaaa tctgcgtgca cgggtcagtg
    17401 gttttatggc aatagaggtg cccacggcgc aggtttccgt gtcagatgcg gtgtctgctt
    17461 atctgtttaa cagccaactg ctgagccgcg atgatggttc catgatgttg gtgctgcctc
    17521 aggaatgtcg ggaacatgct ggagtatggc gttatctcaa tgaattgctt gctgctgaca
    17581 acccaataag ctcactaaaa gtttttgatt tacgtgaaag catggcgaat ggcggtggcc
    17641 cggcgtgcct gcggttgcgc gtggtgttga cagaagaaga acgccgggcg gtgaatccgg
    17701 cggtgatgat gaacgatacg ctgtttaatg cgctcaatga ctgggtggat cgttactacc
    17761 gcgatcgtct tactgcggcg gatcttgccg atccacaatt gctgcgcgaa ggaagggaag
    17821 cgctggatac actgactcaa ttattggatc ttggttcggt ttatccattc cagcgcgagg
    17881 gagggggcaa tggataattt tcttgctcta accttaacgg gtaaaaaacc ggttataacc
    17941 gagcgagaaa tcaacggcgt tcgctggcgc tggctgggcg atggtgtgct ggaactgacg
    18001 ccattaacgc caccgcaagg cgcgctggtg atttcagcgg ggatccacgg taatgagacg
    18061 gcacctgtgg aaatgctgga cgcgttgctt ggcgcgatat ctcacggtga gatcccgtta
    18121 cgttggcggt tactggtgat cctcgggaat cctcctgcgc tgaagcaagg gaaacgttat
    18181 tgccatagcg atatgaatcg aatgtttggc ggtcgttggc aactgtttgc tgaaagcggg
    18241 gaaacctgtc gggcgcgcga actggaacag tgcctggaag atttttatga ccagggcaaa
    18301 gaatctgtgc gctggcacct tgatctacat accgcaattc gtggctcctt gcatccgcag
    18361 ttcggtgtat taccgcaacg cgacattccc tgggacgaga aatttctgac gtggctgggt
    18421 gcggcggggc tggaggcgct ggtgttccat caggagcctg gtggtacgtt tacccatttc
    18481 agcgccagac attttggcgc gctggcctgt acgctggaac ttggcaaagc gttgcccttt
    18541 gggcaaaacg atcttcgcca gtttgcagta actgccagcg caattgctgc gctgctatct
    18601 ggtgagagtg tcggtatcgt gagaacaccg ccgctccgtt atcgggtggt ttcgcaaatt
    18661 actcgccact cgccgtcctt cgaaatgcat atggcaaatg acacgctgaa ttttatgccg
    18721 tttgagaaag gaacattgct ggcgcaggac ggggaggaac gttttaccgt aacccatgat
    18781 gtagagtatg tgttattccc taatccgttg gtagcgttgg gattacgtgc gggattaatg
    18841 ctcgaaaaaa taagctaata cttacccgca gaaa
//
LOCUS       DAZFRG010000084        18612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFRG010000084 DAZFRG010000000
VERSION     DAZFRG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.084"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..384
                     /gene="cmtB"
                     /locus_tag="ACHKUC_002260"
     CDS             <1..384
                     /gene="cmtB"
                     /locus_tag="ACHKUC_002260"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO6618514.1"
                     /translation="WQEAINFSMASLLAKNYISENYIQAIKDSTISNGPYYILAPGVA
                     MPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELL
                     CEEEILEQLLTASSEKQLADIISRG"
     gene            412..1800
                     /gene="cmtA"
                     /locus_tag="ACHKUC_002261"
     CDS             412..1800
                     /gene="cmtA"
                     /locus_tag="ACHKUC_002261"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO6618515.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1815..3092
                     /gene="yggP"
                     /locus_tag="ACHKUC_002262"
     CDS             1815..3092
                     /gene="yggP"
                     /locus_tag="ACHKUC_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6618516.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLTQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LAWLVEKTHGIWNEQAEKYLLAQFGVYIGEAAQ"
     gene            3089..3404
                     /locus_tag="ACHKUC_002263"
                     /pseudo
     CDS             3089..3404
                     /locus_tag="ACHKUC_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3426..3935
                     /gene="fumE"
                     /locus_tag="ACHKUC_002264"
     CDS             3426..3935
                     /gene="fumE"
                     /locus_tag="ACHKUC_002264"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO6618517.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQN
                     EMPGFADDMAWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            3932..4645
                     /locus_tag="ACHKUC_002265"
     CDS             3932..4645
                     /locus_tag="ACHKUC_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO6618518.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(4617..5294)
                     /locus_tag="ACHKUC_002266"
     CDS             complement(4617..5294)
                     /locus_tag="ACHKUC_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6618519.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQDAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVASLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(5288..5965)
                     /locus_tag="ACHKUC_002267"
     CDS             complement(5288..5965)
                     /locus_tag="ACHKUC_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6618520.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(5953..6660)
                     /locus_tag="ACHKUC_002268"
     CDS             complement(5953..6660)
                     /locus_tag="ACHKUC_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO6618521.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     TMILLMLAEFGAWIWLR"
     gene            complement(6661..7239)
                     /locus_tag="ACHKUC_002269"
     CDS             complement(6661..7239)
                     /locus_tag="ACHKUC_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO6618522.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTTVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7266..7694)
                     /locus_tag="ACHKUC_002270"
     CDS             complement(7266..7694)
                     /locus_tag="ACHKUC_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO6618523.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQSCALSGYDELHISPR"
     gene            8066..9085
                     /gene="epd"
                     /locus_tag="ACHKUC_002271"
     CDS             8066..9085
                     /gene="epd"
                     /locus_tag="ACHKUC_002271"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO6618524.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9135..10298
                     /gene="pgk"
                     /locus_tag="ACHKUC_002272"
     CDS             9135..10298
                     /gene="pgk"
                     /locus_tag="ACHKUC_002272"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO6618525.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            10513..11592
                     /gene="fbaA"
                     /locus_tag="ACHKUC_002273"
     CDS             10513..11592
                     /gene="fbaA"
                     /locus_tag="ACHKUC_002273"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6618526.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            11783..12643
                     /gene="mscS"
                     /locus_tag="ACHKUC_002274"
     CDS             11783..12643
                     /gene="mscS"
                     /locus_tag="ACHKUC_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6618527.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            12782..13417
                     /gene="argO"
                     /locus_tag="ACHKUC_002275"
                     /pseudo
     CDS             12782..13417
                     /gene="argO"
                     /locus_tag="ACHKUC_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
     gene            13510..14250
                     /gene="yggE"
                     /locus_tag="ACHKUC_002276"
     CDS             13510..14250
                     /gene="yggE"
                     /locus_tag="ACHKUC_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO6618528.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            14408..15298
                     /gene="ygfI"
                     /locus_tag="ACHKUC_002277"
     CDS             14408..15298
                     /gene="ygfI"
                     /locus_tag="ACHKUC_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6618529.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASRFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFNISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPT
                     IIYRPYSFMAKISAVEREHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRN
                     HRYTATMLQHIAEDRDGTNH"
     gene            complement(15295..15447)
                     /locus_tag="ACHKUC_002278"
                     /pseudo
     CDS             complement(15295..15447)
                     /locus_tag="ACHKUC_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
     gene            complement(15472..16351)
                     /locus_tag="ACHKUC_002279"
                     /pseudo
     CDS             complement(15472..16351)
                     /locus_tag="ACHKUC_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
     gene            complement(16554..17447)
                     /gene="argP"
                     /locus_tag="ACHKUC_002280"
     CDS             complement(16554..17447)
                     /gene="argP"
                     /locus_tag="ACHKUC_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO6618530.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            17588..17818
                     /locus_tag="ACHKUC_002281"
     CDS             17588..17818
                     /locus_tag="ACHKUC_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6618531.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            17874..18533
                     /gene="rpiA"
                     /locus_tag="ACHKUC_002282"
     CDS             17874..18533
                     /gene="rpiA"
                     /locus_tag="ACHKUC_002282"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO6618532.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
BASE COUNT         4581 a         4510 c         4956 g         4565 t
ORIGIN      
        1 tggcaggaag ctatcaattt ctcgatggca tcactgttag caaaaaacta tatcagcgag
       61 aattacattc aggctattaa agattccacc atcagcaatg gtccttatta tattctcgca
      121 ccaggcgtgg caatgcctca tgcgcgaccg gaatgtggag cgcttaaaac cgggatgtct
      181 ttgacgttac ttaaacaagg cgtttatttt ccggggaatg acgaaccgat taaattactc
      241 atcggacttt ctgctgccga tgccgattcg cacattggtg ctattcaggc gttaagtgaa
      301 ttactgtgcg aagaagaaat actcgaacaa ctcttaaccg catcatcaga aaaacaatta
      361 gcggacatta tcagccgcgg ataattactc tctcctctac aggataatat tatggaaaac
      421 aagtctgctc gtgcaaaggt ccaggctttt gggggatttt tgactgcaat ggtcatcccc
      481 aatatcggtg cttttattgc ctggggattt ataacggcat tatttattcc caccggctgg
      541 ctgccgaatg aacatttcgc caaaattgtt ggtccgatga ttacctattt attgcccgtg
      601 atgattggtt ctaccggtgg tcatctggtc ggcggtaaac gcggggcggt catgggcgga
      661 ataggtacta ttggtgtgat cgtcggcgca gagatcccga tgttccttgg ctcaatgatc
      721 atgggaccgt taggtgggct ggtcataaaa tatgtcgata aggcactgga aaaacgcata
      781 cctgccggtt ttgagatggt tatcaataac ttctcattag gtatcgcggg gatgctcctt
      841 tgtctgctgg gttttgaagt tatcggcccg gcggtgttaa ttgctaatac tttcgtcaaa
      901 gagtgtattg aggcgctggt acatgcgggt tatctacctt tgctgtcagt catcaatgaa
      961 ccggcgaaag tgcttttcct taataatgcg atcgatcagg gcgtctatta tccgctggga
     1021 atgcaacagg cttcggttaa cggtaaatcc atcttcttca tggtggcctc caatccaggt
     1081 cctggtctgg ggctgctgct ggcgtttacc ttgtttggta aagggatgag taaacgttct
     1141 gcgcccgggg cgatgattat tcacttcctc ggtgggatcc acgaactgta tttcccgtat
     1201 gtgctgatga agccgctgac cattattgcc atgattgcgg gcggtatgtc tggcacctgg
     1261 atgtttaact tactggacgg tggtctggtg gctggcccaa gtccggggtc tatctttgct
     1321 tacctggcac tgacgccgaa aggctcgttc ctggcgacaa ttgccggtgt tacggtaggt
     1381 accctggtgt cctttgctat tacttcgctg atactgaaga tggaaaaaac ggtggaaacg
     1441 aagagcgaag atgagtttgc tcagtcagcc aatgcggtta aggcgatgaa acaagaaggt
     1501 gcattctcgt taagccgtgt taagcgtatt gcctttgttt gcgatgcagg gatgggctcc
     1561 agtgcgatgg gcgcaaccac cttccgtaaa cgcctggaaa aagcggggct ggcaatagaa
     1621 gtaaaacatt acgccataga aaacgtgcct gcggatgcgg atatcgtcgt tactcacgcc
     1681 agtctggaag ggcgcgtgaa acgtgtgacg gataaaccac tgatattgat taataactat
     1741 attggcgatc caaaactcga cactttattt aatcaattaa ccgctgaaca taaacactga
     1801 ttacagaggt aaaaatgaaa accaaagttg ctgctattta tggcaagcgg gatgtccgtc
     1861 tgcgcgaatt tgagctgcca gaaattaccg ataatgaact gttagtgaat gtaatttctg
     1921 acagcgtttg tttatccacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtcc
     1981 ccgacgattt agaaaatcat ccggtcatta ccgggcatga atgtgccggg gttattgtcg
     2041 aagtgggtaa aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg
     2101 cgatggggtt accaggcgga tattcagcgg gctacagcta cgaatatttt ggcggtaacg
     2161 ccacctatat gattattccc gaaatcgcca ttaacctggg ctgcgtatta ccgtatcacg
     2221 gctcttattt tgctgcggcc tcgctggcgg aaccgatgtg ctgcattatt ggcgcttatc
     2281 atgccaatta tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggtg
     2341 gcaatattgc gctactggcg tgtgcaggcc cgatgggaat tggcgctatt gattacgcca
     2401 ttaacggcgg catacaaccg gcgcgggtgg tggtagtcga tatcgacgac aaacgcctga
     2461 cgcaggtaca aaagctgctg ccggtggaac tggcggccag taaaggcatt gagctggtgt
     2521 atatgaatac caaagggatg agcgatccgg tccagacgct gcgggcgctg acggatgatg
     2581 ccggatttga tgacgtattt gtctatgcag cggtgccttc agtcgtcgag atggctgatg
     2641 aattactggc ggaagatggc tgcctgaact tctttgccgg gccgacggat aaaaacttca
     2701 aagtgccgtt taatttctac aacgtccatt acaacagtac gcacattgtc ggcacgtccg
     2761 gcggttccac ggacgacatg aaagaggcga ttgcccttag cgccacgggg cagttacagc
     2821 cgtcgtttat ggtgacccat atcggtggcc tggatgcggt gccagaaacc gtgctcaatc
     2881 tgccggatat ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctgaccg
     2941 ccattgccga ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ctggcgtggc
     3001 tggttgagaa aacgcacggt atctggaatg aacaggccga gaaatatctg ctggcacaat
     3061 ttggcgttta tattggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt
     3121 tacggaacag gcagcgttgg ctgcctggcc gcaaaccggc tgcggcgaca aaaataaaat
     3181 tgatggcctt gcggtcaccg cgatgcgcca ggcattaaac gacgttgctt ttcgtgggcg
     3241 agtggttatc ggcgaggggg aacgatatcc tctttagcgc tacgggcgtg acgggcggcg
     3301 agttggtgaa cggtatccaa cagacggcga atggggtgcg gacgcagaca ttactgatcg
     3361 gcggcgcgga ccaaacgtgt aatataatag actccctgca ttgatggtga tctatcggaa
     3421 gaccaatggc gacgctgaca gaagatgatg tgcttgagca actgaatgca caggacaatt
     3481 tattttcatt tatgaaaact gcgcattcta ttttgctcca ggggatacgc cagtttctgc
     3541 cgtcgctgtt tgtcgataac gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg
     3601 cccaaagcgg cccgcttgac gatattgatg ttgcgctgcg tttgatttat gcgctgggga
     3661 aaatggataa atggctgtac gccgatatca cgcatttttc ccagttttgg cattacctga
     3721 acgaacagaa tgaaatgccc ggatttgccg atgacatggc ctgggatttt atcagcaacg
     3781 tcaatagcat aacccgcaat gcgacgctct atgacgcgtt gaaagcgatg aagttcgccg
     3841 atttcgccgt ctggtcagag gcgcgtttta gcgggatggt caaaacggcg ctgacgctgg
     3901 cagtaacgac aaccttaaag gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct
     3961 gaagatacag gcacagtacc agaacgaaga gattgaaaat gtacataaac cgttgctgcg
     4021 tatgctggcg gccttacaga cagtaaatcc gcagcggcgt acggtggttt ttctttgcgc
     4081 tccgccgggg acgggtaaat ctaccctaac caccttctgg gaatatctcg cacaacaaga
     4141 cccggaactg cccgctattc agacactgcc gatggatggt tttcaccatt acaatagctg
     4201 gctggatgcg catcaattgc gccccttcaa aggcgcacca gagacattca acgttgcgaa
     4261 actggcggaa aatctgtgcc gggttgtgga aggggattgt acgtggccgc agtacgatcg
     4321 acaaaagcat gatcctgttg aagatgcgct gcacgttacc gcaccactcg tcatcgtcga
     4381 aggaaactgg ttgttgctgg acgacgagaa gtggtgccag cttgcgcaat tttgtgattt
     4441 ctcgatcttt atcaaagctc ccgcatctgc gttgcgggag cgtctggtgg ggcgcaagct
     4501 ggcgggaggg ttatcgctgg cagacgcaga agccttttat gaccgtaccg acgggccgaa
     4561 tgttcgccgg gtgctggaag agagtctgcc tgcaaatttg accttaatga tgaccgctac
     4621 gggtgaatat cgtctgatgg attaacgttc ctgattacac catcttccag tcgcaccaca
     4681 cgagagaaat gacgccgggt aaatgcggcg tcatgactga ttgctacgac gctcgtgcct
     4741 cgctggcggc acttttccag ccagctttca aacacgctca accaattttc gtcaaaatcc
     4801 cgactcggct cgtccagtag caataggggt ggagagagtg cttccaggct ggcgacggcg
     4861 accatccgtc gctgcgcact atgcagatct aacggatgcg cgctggcagt gtctgctaac
     4921 tggcagcatt gcagggcagc attggtacgt tgagtgattt catccgccgg gcatttttgc
     4981 agtttcaaac caaatgcgat ctcgtcagca acagtgctgt gaaaaagctg gttttccgcg
     5041 tcctgaaaca gtacgccaat ctttgccgcg cgctggcggt ttttcaggtt tttcatcgct
     5101 tgttgttgca gcataaccgt accggctgtg ggcgtaagaa gacctgccat tactcgtaac
     5161 agcgtcgatt ttcccgcgcc attgtcgccg gtcagcgcca accattcacc ttgtttgagt
     5221 tgcagagaaa tgtcgcaaag acaatccgtt gccgcgccag gccagcgata agatatctga
     5281 tttaacgtta acatagtggc tggagcgctc cgttttgcaa ttgccacgct tgctggcagt
     5341 ggttcaggaa aggtgtgtga tgacgttcaa aaagaataat cagcgaacca cgttcaagcg
     5401 cccagtgttg taagcgctgc aacagcattt cgcgggcctg cggcgtcagg cggctaaagg
     5461 cttcatcaag aattaagagc tttggctgca tcgcgatggc gcaggcgatg accacacgct
     5521 gtgtctctcc accggagagt gttgccgggt gacgatggcg caacggctgg cactcggtca
     5581 gggccagggc ggcgtcaatc cgcgccataa tctctttttc agcaagacac agattttctg
     5641 gcccaaaggc aacttcttct tccacgctaa aggtacagcc ggagagctgc aaatagggcg
     5701 attgctggac gagctggatg gtggcggatt gttcattaag cgggagacgc ccaatgggcg
     5761 taccgagaag cgtccccgtg ccggttatct ctcctggtag aaagtccgga taccagcccg
     5821 ccatcagctg cgccagcgta cttttgccac tgccgttgtc accaaagatg gcgaccatcc
     5881 ccggtttgac atagtgaaag tcataacaga acggaggatg cgttgagtgt gtcgggcaat
     5941 agcgaaactg ttctaacgta accatatcca cgctccgaat tcagccagca ttagcaatat
     6001 catggtgtaa cgtgctacgc gttgtagggt gctatcggct ggtgcccata acgtagtgcg
     6061 cctattgttg attcgaaatg cccgcatatc cagcgccgca ccacggacag caagatcgtt
     6121 tagcgcattg tgtgttagcg gaataattaa cgcgggcatc gcacgtaaac gctgatacca
     6181 gccctcatcc agcggcacgc cgcgggcgcg ctgcgcttca tgaataatcg ccaattgccg
     6241 ttttaactgc tcaacgacca ataacggccc ggcaaacaga taagcgacgc ccggcggcag
     6301 gcgagaagca aacaaagcgc ggataaaacg ttgcacgggg acgtactgca tccacaactg
     6361 tgaggttgaa acaatcgcca gaatccgcaa ccagagcgta atagcatgcg cccaccgttc
     6421 tggagagcgt ggcgtgccgc ttagccattc tgtcagccag ccaccgtgca ccagccacaa
     6481 tccagccccc agcgagaaca tcagccagac gacatatttt gcccgccgcc gcgtggcctt
     6541 tagcgcaata aggcagaaaa aggtcgccgc gctatagact ggcaatattg tctgtgtggg
     6601 caggataagc gtggtgcagg ccgccagcgc ccacagcgtt aaggatgtaa acggatgcat
     6661 ttagcgcacg gcagccattg ccggaaaatg gcgcgtggtg cgaatcggca gctgacgcag
     6721 taaaagccag acaatcaccg ttgtgagaat tttatcgacc aggttcgcgc ctatcaccgt
     6781 aatcgccacc gactccacca ggttttgccc catcgagtgc atccaggcaa cgaacaaatc
     6841 ggctccgctt cctgtcacgc cgccgaacag cgcagtacgc agcggtacag cgacgaccgt
     6901 taccgcaagc gtaataatca cgccgctaac aacgactttt ggcagggtgc gaaaccaacc
     6961 cgctcgcgcc agccaaccgg cgaccaggcc gatgaccatc gcgacgggcg caaatgcggc
     7021 ggcgatcggg tcggtcagca atccccagag taaattggtc agtaaaccgg ttagcatccc
     7081 gatcaccgga ccaagcagta ccgcgctaat aagtgtgccg atggagtcga gaaaaatagg
     7141 taatttgacc atgctggcca gttgcccgcc gatcatattg atggcgatag agataacgat
     7201 caacaccaat gcctgactgg aaaagtggcg acgcgccata agaaaacctc actgaaagta
     7261 gttatttaac ggggggaaat atgcaactcg tcgtaaccag aaagcgcgca agactggcgg
     7321 ctaaacgcca gttcctcaag gcagccgatc accagttcaa gcgtggtccg cacggtacag
     7381 ccgggcatca tatgaccgcc aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga
     7441 tgttcaccac tctgttccag cgtgccgttc aatgcgatga cttcgaactt accgcttagc
     7501 agggcagtgt tttcttgccc ggcatagcgt aaggcgacgt cagtcagact gccggtacaa
     7561 cctgcaatcc acgcggcgtg gagttgttgt tgttgcgcga aggcgcgtaa ttgggagaga
     7621 acttcctgcc cggggagcag gcgcagcgcg taaaaacgtg cggaggaaga atatgggtag
     7681 ggcaatgtca tcattggagt actccgaaac tcggacgtat tgcggataat atcgacgctg
     7741 catcagtcat attctgatat aagtcagggt ttgtgacggc acaacgaaaa gattttgcta
     7801 cgcttattcg tgattctgca aagtggaata aattccgcag gggcggggct gaaaacgtga
     7861 cgtaagtcag ataattgtct tatttgactg gacaatcatt ccttttattc cacgtttcgc
     7921 ttatcctagc tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag
     7981 tttgcgacag gtaaggtttc cccgaacgat ttgctggatt actctgtctg gcaaatttgc
     8041 tcagggaaaa ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg
     8101 catcgggcgt aatgtggttc gtgctttgta tgaatccgga cgccgtgcgg aaattaccgt
     8161 ggtggcaatc aacgaactgg cggatgctgc gggtatggcg catttgttga aatatgacac
     8221 cagccatggc cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga
     8281 tgacgccatc cgtgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg
     8341 cgttgatgta gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gtgaagcaca
     8401 cattgccgcc ggggctaaaa aagtgctctt ttcacatcct ggcagtaacg atctcgacgc
     8461 gaccgttgtt tatggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa
     8521 cgcttcctgt accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg
     8581 tattgagtcc ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga
     8641 tgcataccat cctgacctgc gtcgcactcg ggcagccagt cagtcgatca ttccggtcga
     8701 tactaaactg gccgccggta tcacacgatt ttttccgcaa tttaacgatc gctttgaggc
     8761 gattgcggta cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa
     8821 gaaacctgta aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt
     8881 tcatggtata gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc
     8941 gcacagtgcc attgtcgatg gcactcaaac ccgggtcagt ggcgcacacc tgatcaaaac
     9001 gttggtctgg tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc
     9061 tatggctact gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa
     9121 cgagaggatt caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg
     9181 tatttatccg tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc
     9241 gtatccgtgc ttctctgccg accatcgaac tggccctgaa acaaggcgca aaagtgatgg
     9301 taacttccca cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc
     9361 cggttgttaa ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc
     9421 tcgacggcgt tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca
     9481 aaggcgagaa gaaagatgac gaaactctgt ccaaaaaata cgctgcactg tgtgacgtgt
     9541 tcgtaatgga cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta
     9601 aattcgctga cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta
     9661 aagcactgaa agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta
     9721 ccaaactgac cgttctggac tccctgtcta aaatcgctga ccagctgatc gttggcggtg
     9781 gtatcgctaa cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag
     9841 ctgacctggt tgacgaagct aaacgtctgc tgaccacctg caacatcccg gttccgtctg
     9901 atgttcgcgt agcaaccgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg
     9961 atgtgaaagc tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg
    10021 aaatcctgaa gaatgcgaaa accattctgt ggaacggtcc ggttggcgtg ttcgaattcc
    10081 cgaacttccg caaaggtact gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct
    10141 ccatcgctgg cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa
    10201 tctcctacat ctccactggc ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc
    10261 ctgcagtagc gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc
    10321 tgcccttgtt tcagcgcgct tttagagcac gcactttttc atactctaaa taattcgagt
    10381 tgcaggaagg cgacaagaga gtgaatcccc aggagcttac ataaataagt gattggggtg
    10441 agcgaacgca gacgcagcac atgcaacttg aagtatgacg agtataaggc ccgacgatac
    10501 aggacaagag acatgtctaa gatttttgat ttcgtaaaac ctggcgtaat tactggtgat
    10561 gacgtacaga aagttttcca ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac
    10621 tgcgtcggta ctgactccat caacgccgta ctggaaaccg cagctaaagt taaagcgccg
    10681 gttatcgttc agttctccaa cggtggtgct tcttttatcg ctggtaaagg cgtgaaatct
    10741 gacgttccgc agggcgctgc tatcctgggt gcgatctctg gtgcgcatca cgttcaccag
    10801 atggctgaac attatggtgt tccggttatc ctgcacaccg accactgcgc gaagaaactg
    10861 ctgccgtgga tcgacggtct gctggacgcg ggtgaaaaac acttcgctgc taccggtaag
    10921 ccgctgttct cttctcacat gattgacctg tctgaagaat ctctgcaaga gaacattgaa
    10981 atctgctcta aatacctgga gcgcatgtcc aaaatcggca tgactctgga aatcgaactg
    11041 ggttgcaccg gtggtgaaga agacggcgtg gacaacagcc acatggacgc ttctgcactg
    11101 tacacccagc cggaagacgt tgattacgca tacaccgagc tgagcaaaat cagcccgcgt
    11161 ttcaccatcg ctgcgtcctt cggtaacgta cacggtgttt acaagccggg taacgttgtt
    11221 ctgactccga ccatcctgcg cgactctcag gaatatgttt ctaagaaaca caacctgccg
    11281 cacaacagcc tgaacttcgt cttccacggt ggttccggtt ctactgctca ggaaatcaaa
    11341 gactccgtaa gctacggcgt agtgaaaatg aacatcgata ccgataccca atgggcaacc
    11401 tgggaaggtg ttctgaacta ctacaaagaa aacgaagctt acctgcaagg ccagctgggc
    11461 aacccgaaag gcgaagatca gccgaacaag aaatactacg atccgcgcgt atggctgcgt
    11521 gccggtcaga cttcgatgat cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc
    11581 gacgttctgt aagatattcc tttctgctta tctcaaggcc cgctctgcgg gtcttttttt
    11641 cgccaaaagc aaggtgatac agatgagaaa tctgtgatct atttggcaaa attatgcttt
    11701 attgtttacc cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga
    11761 gtcagttgaa aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggcgc
    11821 gggaagctgg ctggtggcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc
    11881 ggcactcgcg atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa
    11941 tcgcctgatg atctcccgta aaatcgatgc cactgttgct gattttcttt ctgcattagt
    12001 ccgttacgga attatcgcct ttacgctaat cgcagcactg ggacgcgtgg gcgtacaaac
    12061 agcgtcagtc attgctgtac tcggtgccgc aggtttagct gttggtctgg ctttgcaggg
    12121 gtcactgtct aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg
    12181 agaatatgtt gacctcggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac
    12241 caccatgcgt actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg
    12301 gaatattatt aatttctccc gcgagccagc tcgccgtaac gaatttatta ttggcgtggc
    12361 gtatgattcc gatatcgatc aggttaagca gatcctgacc gatattatcc agtctgaaga
    12421 tcgcattttg aaagatcgcg aaatgactgt gcgtctgaac gaacttggcg catcgtcgat
    12481 taatttcgtg gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt
    12541 gctggagcgt attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat
    12601 ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga
    12661 taatactctc tggcctctct cttattagtt tttctgattg ccaattaata ttatcaattt
    12721 ccgctaataa caatcccgcg atatagtctc tgcatcagat acttaattcg gaatatccaa
    12781 cgtgttttct tattactttc aaggtcttgc acttggcgca gctatgatcc taccgctcgg
    12841 tccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca ttatgattgc
    12901 cttactttgt gctatcagcg atttggtcct gatttgtgcg gggatttttg gtggcagcgc
    12961 gttattgatg cagtcgccgt ggttgctggc gctggtcacc tagggcggcg tagccttctt
    13021 gctgtggtat ggttttggcg cttttaaaac agcaatgagc agtaacattg agttagccag
    13081 tgccgaagtc ctgaaacaag gcagatggaa aattatcgcc accatgttgg cagtgacctg
    13141 gctgaatccg catgtttacc tggatacttt tgttgtgctg ggcagccttg gcgggcaact
    13201 tgatgtggaa ccaaaacgtt ggtttgcgct cggaacaatt agcgcctctt tcctgtggtt
    13261 ctttggtctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca
    13321 gcgcattatc aatctggtag tgggatgtgt tatgtggttt attgccttgc agctggcgag
    13381 agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa
    13441 acagaggcgc taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga
    13501 gagactaacg tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg
    13561 gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagc
    13621 gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat
    13681 gccgctactg ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgag
    13741 ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaact tacgcactca gccagattat
    13801 gattatcagg atggtaaaag tatcctgaaa ggctaccgcg cagtgagaac ggtggaagtc
    13861 acgctccgtc agttagacaa actgaattcc ttgctggacg gcgcgctaaa ggcgggtctt
    13921 aacgaaattc gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg
    13981 cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa tggctttaat
    14041 cgcaaactgg ggccggtata tagcgtgcgt tatcatgttt ccaactatca gcccagccca
    14101 atggtgcgga tgatgaaagc tgatgctgca ccggtgtccg cacaggaaac ttacgagcag
    14161 gccgctattc agtttgatga tcaggtcgat gtagtcttcc agttagaacc tgtggatcaa
    14221 ccccccgcta aaacacctgc tgcacattaa tacttgtagc ccgacgtatt cgtcgggcta
    14281 atgtctttac ccgatattgt ctcctttttc tgtaaaacca tgacgtaatc acctatcttt
    14341 gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag gatgcgggag
    14401 aggggggatg gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac
    14461 caataatatt gcccgggcgg cagaaaaaat ccatatgacg gcttcgcgat ttggcaaaag
    14521 cattgccgcg ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat
    14581 cagcctcaat aaagccggac aggaactgta tcaaaaactg ttcccggttt atcaacgact
    14641 ttctgcgatt gataatgaaa tccataactc aggacgtcgt tcacgagaaa ttgtgatagg
    14701 catagataat acttatccga ccattatttt tgatcagttg attagtctcg gagataagta
    14761 cgaaggcgtg accgcccagc cagttgagtt cagcgaaaat ggcgttatcg ataatctttt
    14821 tgaccgtcag ttagatttta atatctcccc acagcatgta tctgcacgag ttcaggaact
    14881 ggaaaacctg actatcagtg aactaccgcc gctgcgattg ggctttctcg tttctcgccg
    14941 ttatgaagag cgacaagagc tgcttcagga gctgccctgg ctacaaatgc gctttcagaa
    15001 tcgggccaat ttcgaagcaa tgattgatgc gaatatgcgt ccttgtggga tcaatccaac
    15061 cattatttat cgtccctaca gttttatggc caaaatcagc gccgtggagc gggaacattt
    15121 tctgacggtg atcccgcatt ttgcctggcg actggtgaat ccggcaacgc tgaaatattt
    15181 tgatgcgccg cacagaccga tgtatatgca ggaatatctt tattcaatca gaaatcatcg
    15241 ttataccgcc acgatgcttc agcatattgc tgaagatcgt gacggaacaa accattaacc
    15301 cagcatcgat ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc taagatcgtg
    15361 gtgaatatgt ccgccggacg cattttccag atagcgatgc agataatccc gacacatagg
    15421 atgtgcacaa tgatcaatgg tggtgcggat aacgttgtta tacggatcct gtgcgccaaa
    15481 tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcgg cgttcataaa
    15541 cagatccatg ccaatgccga agaaaaacga caggcgaggg acgaagccat caattttcag
    15601 cccggcggag attgctgctt tgatgtactc aatcccatcg gcgagcgtaa atgctacctg
    15661 ctgcacgcag ttggcacccg cttcacccat gtggtaaccg ctgatactga tggtattaaa
    15721 tcgcggcatg ttgccggaac accaggcgat gatgtctgcg ataatacgca ttgacggttt
    15781 tggcgggtaa atataggtgt tgcggcaaag atactctttg aggatatcgt tttgaatggt
    15841 gccggtcagt ttatcgggtg taacaccttg ctcttctgcg gcgacaatat aaaacgccag
    15901 tactggtagt actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg
    15961 gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt tgccgacgtc
    16021 gcccgccacg cgtgggttat cggagtcgta gcctcggtgg gcggccaggt tacggcgata
    16081 aaaagcgttg gactcctttt gttgtcgaga aaccagcata ctgacggatg gtccacggtt
    16141 gggcggtata catagtggca cgcgggccac gaacgtaggg tggtaaaccg ggaagggtac
    16201 ccgtcacctc cagattatcg agatcggctt cggtatacag cggcttgata gcgatccctt
    16261 ccgcggtttg ctgaaccagc gagtcgacag ttttctcccg acggctcaat tccttgttgg
    16321 caagctgttg ccactcctgc acgttagaca ttatttgctc cgtaaaacgc aatcaaaatg
    16381 agggtatttg cgcttacggt gagcttttta cgtgggtttg tcgccagcaa aaaaatgcat
    16441 tcagacgtcg ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac
    16501 aaaaagaagt gaggataacg cctgatatgt gctatcaggc gttatttgat ggattaatcc
    16561 tgacgaagaa ctttgtgacc ataatcgagc aacgcatcag tgactttacg catcatgcgg
    16621 ctttcaggag caaagcggtg ccagtagagc atccggcgtt gaaatagccc tggcgttaag
    16681 tcaatcagtt cgccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag
    16741 gtggtgccct gacgagcaag ttgcacgaac gcttccgaag aattaacgat atggcagggt
    16801 acgctgccag gaggcagatc gaaattttgc tgcaaaaagg cctggtgcat atcgtcaaga
    16861 tggtcaaacg cgaccactgg cgctttcagt aatgccgaac gcgttacgcc gttagggaaa
    16921 tatttttcgg caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg
    16981 acaagacaac tcggcagcgc ctgatgttga atactcactg cgccgaccac ttcgccgcga
    17041 cgcaaacgtt cctgagtgcg ggtttcatct tctacctgca agttgaggcg gatgggcgaa
    17101 tcagccaaca caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc
    17161 gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc
    17221 agcagctcca cctggcgtag tagcgccaac agtttttgcc cttgttccgt cgggcgcggc
    17281 ggtacggtac gcactaacaa cggctgcccg aacatatttt ccagttgctt aatgcgctgt
    17341 gagacggctg attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt
    17401 atcaccgcat ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc
    17461 tcctggtaaa tgctttttcc tgcactatga cacaattttg tgtcgggttg catataccga
    17521 agccgttggc gggtaacatt tgtatcagtg gttaacgcgc agcttaaaag atcagggatt
    17581 tgcgctaatg catttcgctc agcgagttcg cgccctggtt gtgttgaatg gcgtggcgtt
    17641 attgcctcag tttgcctgta aacaggggct tgcgaacggg gaactggtgc gcctgtttgc
    17701 accgtggagc ggcatcccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc
    17761 cgccattgcg cgatatttta tggatgagtt aaccacgcgg cttgccaacg gggtctgaat
    17821 cacttttttt gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc
    17881 aggatgaatt gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggcacca
    17941 ttgttggtgt aggtacaggt tccaccgccg cacactttat tgacgcactc ggtacaatga
    18001 aaggccagat tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc
    18061 tcggcattca cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg
    18121 cagatgaaat caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg
    18181 aaaaaatcat tgcttcggtt gcggaaaaat ttatctgtat tgcagacgct tccaagcagg
    18241 ttgatattct gggcaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag
    18301 tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtagtgaccg
    18361 ataacggcaa cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg
    18421 aaaacgccat aaatgcgatt cctggtgtgg tgactgttgg tttgtttgct aaccgtggcg
    18481 cggacgttgc gctgattggc acacctgacg gtgtcaaaac tattgtgaaa tgatctgacg
    18541 ggggaacctc ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgttt
    18601 ttacctggca at
//
LOCUS       DAZFRG010000085        18485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFRG010000085 DAZFRG010000000
VERSION     DAZFRG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.085"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..107
                     /locus_tag="ACHKUC_002283"
     CDS             <1..107
                     /locus_tag="ACHKUC_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO6618533.1"
                     /translation="VKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(158..964)
                     /gene="trpA"
                     /locus_tag="ACHKUC_002284"
     CDS             complement(158..964)
                     /gene="trpA"
                     /locus_tag="ACHKUC_002284"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO6618534.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEVLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(964..2157)
                     /gene="trpB"
                     /locus_tag="ACHKUC_002285"
     CDS             complement(964..2157)
                     /gene="trpB"
                     /locus_tag="ACHKUC_002285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO6618535.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2169..3527)
                     /gene="trpCF"
                     /locus_tag="ACHKUC_002286"
     CDS             complement(2169..3527)
                     /gene="trpCF"
                     /locus_tag="ACHKUC_002286"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO6618536.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFTNGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTREQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(3531..5126)
                     /gene="trpD"
                     /locus_tag="ACHKUC_002287"
     CDS             complement(3531..5126)
                     /gene="trpD"
                     /locus_tag="ACHKUC_002287"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO6618537.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLKPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5126..6688)
                     /gene="trpE"
                     /locus_tag="ACHKUC_002288"
     CDS             complement(5126..6688)
                     /gene="trpE"
                     /locus_tag="ACHKUC_002288"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO6618538.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGYLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6710..6803)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6780..6824)
                     /gene="trpL"
                     /locus_tag="ACHKUC_002289"
     CDS             complement(6780..6824)
                     /gene="trpL"
                     /locus_tag="ACHKUC_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO6618539.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6962..7843
                     /gene="rnm"
                     /locus_tag="ACHKUC_002290"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6962..7843
                     /gene="rnm"
                     /locus_tag="ACHKUC_002290"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO6618540.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALESAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7840..8460
                     /gene="yciO"
                     /locus_tag="ACHKUC_002291"
     CDS             7840..8460
                     /gene="yciO"
                     /locus_tag="ACHKUC_002291"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO6618541.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8488..10377
                     /gene="yciQ"
                     /locus_tag="ACHKUC_002292"
     CDS             8488..10377
                     /gene="yciQ"
                     /locus_tag="ACHKUC_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO6618542.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLTFLFCSLDLLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGVYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            10590..11465
                     /gene="rluB"
                     /locus_tag="ACHKUC_002293"
     CDS             10590..11465
                     /gene="rluB"
                     /locus_tag="ACHKUC_002293"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO6618543.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTINFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(11505..12095)
                     /gene="cobO"
                     /locus_tag="ACHKUC_002294"
     CDS             complement(11505..12095)
                     /gene="cobO"
                     /locus_tag="ACHKUC_002294"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6618544.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(12092..12850)
                     /gene="yciK"
                     /locus_tag="ACHKUC_002295"
     CDS             complement(12092..12850)
                     /gene="yciK"
                     /locus_tag="ACHKUC_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO6618545.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEEIGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVTQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13070..14119
                     /gene="sohB"
                     /locus_tag="ACHKUC_002296"
     CDS             13070..14119
                     /gene="sohB"
                     /locus_tag="ACHKUC_002296"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO6618546.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKSLM"
     gene            complement(14155..14406)
                     /gene="yciN"
                     /locus_tag="ACHKUC_002297"
     CDS             complement(14155..14406)
                     /gene="yciN"
                     /locus_tag="ACHKUC_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO6618547.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14786..17383
                     /gene="topA"
                     /locus_tag="ACHKUC_002298"
     CDS             14786..17383
                     /gene="topA"
                     /locus_tag="ACHKUC_002298"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO6618548.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            complement(17438..18346)
                     /locus_tag="ACHKUC_002299"
     CDS             complement(17438..18346)
                     /locus_tag="ACHKUC_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO6618549.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
BASE COUNT         4472 a         4590 c         4900 g         4523 t
ORIGIN      
        1 aagtgaaata taaatccaac ggcgcagtgc tgcctgcagg gcgttattct gacaacgtac
       61 gccttgaccc gtgggtattt atgttctctg cgggttaccg cttctaattt tttaccatca
      121 ataaaccgcc agatgttttc tggcggtttg tctcaaatta actgcgcgtc gccgctttca
      181 tcggttgtac aaaagctttc agtgccgcca gcattttctc tggctcatta atatgttgct
      241 cgatgatttt aacgatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgcgg
      301 cttttacctg atccggggcg gaaataccaa atccctgcaa tggaggcgca gcgttgtact
      361 ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca
      421 cgcccgctcg cgacagcaaa taggtgtaac cacgaccgta agaggctatc tggcgcagca
      481 aatcatcgtc ggcattcggc gggcaaataa agataggtgc gacattatga cgcaacgcgg
      541 cctggcggaa gggcgcggac tcttccacgg gcacatcggc aaccagcacc gaatcgacgc
      601 cgactttctc gcactcggca taaaactcat caatgccttt gttaaacacc aggttggcat
      661 acatcaaaag gccgatggga atggtcgggt gcttctggcg aatgagtgcc agcacctcaa
      721 agcactgcgc cggggttact cccgccgcaa aagcacgcag tgtggcgttt tgaatcgtcg
      781 ggccatccgc cagtgggtcg gagaagggga tgcctaactc cagcgcgtca gcaccggctt
      841 caattagcgt atcgataatt ttcaacgact gctcaatgcc cggatcaccg agggtgacga
      901 aaggaacgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt
      961 ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc
     1021 gcgaccggaa aggttaacca ccagtagctg ctctttttcc ggattttcgc gcatcatttt
     1081 cagcgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt catgcaggca
     1141 aagcgtttta aaggcttcca gggcttcatc gtcggtaata gacacgtaat cagcgcgtcc
     1201 agtgctgttg agatacgcat gttgcgggcc gacggacggg aaatccagcc cggcagaaat
     1261 ggagtaagat tcttcaattt gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa
     1321 atagatgccc acgcgaccat gttttaacgg tgcgccgtgc tcgccagttt cgataccgtg
     1381 gccgccaggc tccacaccaa tcaggccgac gtcggtttcg ttgatgaaat ctgcaaacat
     1441 accgatggca ttcgaaccac cgccaacaca ggcgataacg gcatccggca ggcgaccttc
     1501 tctttccaga atctgcgctt tcgtttcttc gccaatcatc cgctgaaact cacgcacaat
     1561 ggtcgggtaa ggatgcgggc cagctgcggt acccagcata tagtgcgcgg tttcataact
     1621 gccggaccag tcgcgtagcg cctcattaca ggcatctttc agggtcgcgg aaccgctatg
     1681 taccgggatc acttccgcac ccattaagcg catccggaaa acgttgggcg actggcgttc
     1741 aacgtctttg gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc
     1801 cgacgccacg ccatgctgac cggcaccggt ttcggcaata atttcagttt tacccatccg
     1861 cttcgccagt aaagcctgac cgagcacctg gttagtttta tgcgcgccgc cgtgcagcaa
     1921 atcttcgcgc ttcagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt
     1981 cagcgcggtt ggacgcccgg catagttttt cagcaggtcg ttgaactgag cctgaaattc
     2041 aggatctttt tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat
     2101 ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt
     2161 tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg
     2221 atacccggtt gcgactctac agcagaatta aaatcaagcc cggcgcagcc ggtttgtgcc
     2281 gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga
     2341 ccatttaata gtgaccagtc gaaacgttgt ccgctcccgc cctgaccgtt gtcgaataca
     2401 tatttatcga tgtgctgaaa atcgcgcgcg ggaagagttt caccgacact taaagccttc
     2461 cagatggcga cgtgtgctgg cagagcctca cgcagagtgt cgatatacag ctgatcttca
     2521 ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca
     2581 atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc
     2641 gcctgttcaa cgttgacgca acgcggtgat gtcgcaacaa aaatcagccc accgtaaatc
     2701 gcgcccgcgt cataagctgc tttagcatct tgttcacgcg tcaggccaca tactttattc
     2761 tcacccagca acacccgacg cacggcggcg ttcaaatcgt catgggccat caacgccgaa
     2821 ccaatcagaa agccgttagt gaagtggctt aactcgcgca cctgagcgta agtattgatg
     2881 ccggattcgc tgattaccgt cacgttgtgc cccagtttcg gtgcaagctc gcgggtacgg
     2941 ttgagatcaa tcgacaaatc gcgcagatcg cggttgttga tgccaacgac ctttgccccc
     3001 aatgcaatgg cgcgctccag ttcctcttca ttactgactt cggtcagcac acccatctcc
     3061 agactgtgcg cgacggctgc aagctggcga tattgttcgt catccagtac tgaaagcatt
     3121 aataagcagg catcggcctg gtaatagcgc gccagataga tctggtaagg atcgataatg
     3181 aagtctttac ataaaatcgg ctgcggggcg atttggctga cgatggggag gaaatcaaag
     3241 ctcccctgaa aatatttctc atcagtcagc actgaaattg ccgaagcgta atgtttataa
     3301 atggcagcaa tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct
     3361 tttttgcact ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgc
     3421 gtgctcggct gaatctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggcttct
     3481 acccaaatcg ccttgtctgc gacgattttc gctaaaacgg tttgcattat ttaccctcgt
     3541 gccgccagtg cggtgactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt
     3601 tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacattcgcc
     3661 gcgacggctg cttcgtgagc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca
     3721 cggttttctt ccggtgttcc gcctgccagt tgctcctggt ggtagggtgt caggccaaaa
     3781 tcttcagcgg tcaattgata gctcttaatt tcgccgtcat gcagttcggc aacgattgtc
     3841 ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac
     3901 cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt
     3961 aacgccagcg gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagtgtgcgg
     4021 gtttttagtt gctggcgaac cggcattgca tggcggaatc cggtgtgata tttcggtgca
     4081 aagaggaaac agacgcctaa ctcatccagc gcctggcgcg atttatcggc gttcatatca
     4141 agattaatac cgaacgccgc cagcagatcc gacgagccgg atttactgga gacgctacgg
     4201 ttgccgtgtt tcgccacttt cagcccgcag gccgcggcga caaacgcact ggcggtagaa
     4261 atattgatgc tgttactgcc gtcaccgccg gtgccgacga tatcggcaaa cagataatcc
     4321 gggcgcggga acggcgcggc gttttccagt agcgcggttg ctgctccggc gatttcgttc
     4381 gggtgctcgc cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggtttcagt
     4441 tcgccacgta ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgggcc
     4501 tgatacagtt tttccagaat cggttgcagc gtgttggttg gctttagttt ctgctgcgcc
     4561 caggccagcg tttgttccag caggcgagcg ccctgggtag taagaatgga ttccggatgg
     4621 aactggaatc cacaaacgcg atccgcatca tgacgcaccg ccatcaccat gccattaaaa
     4681 tgggcgttga tggttaaacc ggccggaata ttactgccaa ccagcgagtg ataacgcgcc
     4741 actggcagcg ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac
     4801 gctttaccgt gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatc
     4861 gcctgatgtc cgaggcaaat gccaataatt ggcagcttgc cacgcaagcg ggtgaggagt
     4921 tccggcatac aaccggcttc gctcggcaca ccggggccag gagaaagcat cagcaccgga
     4981 ttgctcatcg tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc
     5041 accacgttat gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata
     5101 ttatcgagca gcagaatgtc agccattaga acgtctcctg tgcatgatgc gcggtggcaa
     5161 tagcgcgcag tacagcgcgg gctttattac gggtttcgtc tgcttccgac tgcggaacag
     5221 aatcaaggac tacgccagca ccggcttgca cggtggcgat accgttttcc accagcgccg
     5281 agcggatcac aatgcaggtg tcgagatagc catgcgcggt aaaataacct accgcgccgc
     5341 cgtagctgcc gcgtcgacga ccttctgctt cggcaattaa ctgcatagcg cgtactttcg
     5401 gtgcaccgct taacgtcccc atattcatac aggcgcggta agcgtgcagg gcgtcgagat
     5461 cgtggcgcag ctcaccaaca acgcgggaga ccaggtgcat cacgtaagag taacggtcaa
     5521 ctttggtgag atcggcgacg tagcggctgc cgggtgtgca aatgcgtgcc aggtcattac
     5581 gggcgagatc caccagcatc agatgttcag aaagctcttt atgatcggta cgcatctcca
     5641 gttcgatgcg gctgtcgagg tctctatcca gtgaaccatc ggcacgacga ccgcgtggac
     5701 gtgttccggc aatcgggtaa atctcaatct ggcggctggt ggcgtcatac ttgagcgaac
     5761 tttccggcga cgcgccaaac agggtgaaat cattatcctg cataaaaaac atgtacgggc
     5821 tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga
     5881 aacggcgaga tggcaccacc tggaaaattt ctccggcgcg aatcgctttt tgcaacaaac
     5941 gcactacgcc accgaactct tcatcgctct ggttacattc acaacgcata tgcggcacgg
     6001 aaaccaccgg cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag
     6061 cagtgagacg ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc
     6121 tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc
     6181 agctattttc cgctgacagt tgcggtaaat tttcaaatcc cgccacaagg tcataagaga
     6241 acaggccgcc gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgta
     6301 ataagcggaa agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg
     6361 gactgacagg cgggaagcgc agtacgcggc agtttggtga ttgttcattt tccacacccg
     6421 caggcaaggc gttatccagt agtgtcaaca gggcttctcc attgccggaa agcgcctgga
     6481 ttgtgacagt gtcacttaat gctgtaatgc gcagcgcact gtctaccagc agcaggcttt
     6541 ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgatccc
     6601 cacacaactg gtgaaaaagc gcagtcgggt tgtcgcgata agcgcctttg caggttagca
     6661 gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc
     6721 cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt
     6781 caggaagtgc gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt
     6841 acgtgaactt gcgtactagt taactagttc gatgattaat tgtcaacagc tcatttcaga
     6901 atatttgcca gaaccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc
     6961 cttgagcgac acgaattacg cagtgattta cgacctgcac agccatacca cagcttccga
     7021 tggctgcctg acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct
     7081 ggcgatcacc gaccatgaca ccacagctgc aattgcacca gcaagagagg aaatttcacg
     7141 ttcgggactg gcgctgaatc ttattcctgg tgtggaaatt tccacggtct gggaaaatca
     7201 tgaaattcat attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct
     7261 cgcgcagcag acagaacggc gcaatcagcg ggcgcagctg attgccgaaa gacttgaaaa
     7321 agcgcaaata cctggggcac tggaaagcgc gcaacgactg gcgcagggcg gtgcggtgac
     7381 gcgcggtcat tttgcgcgtt tccttgtgga gtgcggaaaa gccagttcaa tggcggatgt
     7441 ctttaaaaag tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat
     7501 agagcaatct attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg
     7561 gcggtacaat cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca
     7621 cggcgacgcg atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc
     7681 gacacttgcg cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg
     7741 cccgtggatc gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca
     7801 gctatgggaa cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt
     7861 catcctgata acccgcagca acgcctgatc aaccaggcgg tggagatcgt gcgtaaaggc
     7921 ggggtgattg tttatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa
     7981 aacgcgatgg agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg
     8041 atgtgtcgcg atctttctga actctcgacc tattcatttg tcgataacgt tgcgtttcgt
     8101 ttgatgaaaa acaacacgcc aggcaactat accttcatcc tgaaggggac gaaggaggtg
     8161 ccacgccgtc tgttgcagga aaaacgcaaa accatcggta tgcgtgtgcc gtctaacccg
     8221 attgcgcagg cgctgcttga agcattgggc gaaccgatgc tttccacttc gctaatgctg
     8281 ccaggcagtg aatttaccga atccgatccg gaagaaatta aagaccgtct ggaaaaacag
     8341 gtggatttga ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctc
     8401 accgatgata cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa
     8461 caaattgcgt tccacggatg gaagatgatg gcagggatat atcgctgcat tttgctgttg
     8521 atagttgggc tttttttctc ttcgttgagt tatgcgaaaa acacggagat cccttcttat
     8581 gaagaaggga tctcgctctt tgatgttgaa gccactctgc aaccgaatgg ggtgctcgac
     8641 atcaaagaga acattcattt tcaggcgcga aatcagcaga ttaagcacgg attttatcgt
     8701 gatttaccgc gactctggat gcagcctgat ggggacgctg cactgctgaa ctatcatatt
     8761 gttggcgtca cccgtgatgg tattcctgaa ccctggcatc ttgactggca tatcgggtta
     8821 atgagtattg tcgtgggcga taaacaacgt ttcttgcctc aaggcgacta tcattatcaa
     8881 attcattatc aggttaaaaa tgctttcctg cgtgaggggg attctgatct gctaatctgg
     8941 aacgtgaccg gtaaccactg gccgtttgaa atttataaga ccctattttc actcaagttg
     9001 cctgatattg cgggtaatcc atttagcgaa atcgatctct ttactggaga agagggcgac
     9061 acatatcgaa atggccgcat ccttgaggac ggaagaattg aatctcgcga tccgttttat
     9121 cgtgaagatt tcacggtcct ctaccgctgg cctcacgcat tacttggtaa tgccccggct
     9181 ccacaaacga cgaatatttt cagtcatctt cttttaccct caacgtcatc gttgttaatt
     9241 tgctttccga gtctgttcct ggcgtgtgga tggttatatc tctggaagcg caggccgcaa
     9301 tttacgccgg tagatgtgat tgaaaccgat gtcattccgc cagattacac gcccggcatg
     9361 ttacgtcttg atgcgaaact ggtttacgac gataaaggtt tttgcgccga tatcgtaaat
     9421 ctgattgtga aagaaaaaat tcatctggaa gatcattatg acaagaacca gcaaatcctg
     9481 attcgtgtta atgaaggcgc gaccagaaat aatgcggtat tactgcccgc agagcagtta
     9541 ttactggaag cgttatttcg taaaggcgat aaggtcgttc ttacggggag acgcaacaga
     9601 gtcttacgca aggcattttt acggatgcag aaattttatc tgccgcgtaa aaagtcttcg
     9661 ttttaccgac ctgatgcgtt tttgcaatgg ggcggaatgg caatattggc ggtcattctc
     9721 tacggtaacc tgagtcccgt aggctgggaa ggaatgagtc tggtaggcga tatgtttatt
     9781 atgatctgct ggcttcttac tttcttattt tgttcccttg atcttttgtt tgcccgcgat
     9841 gatgacaagc cttgcgttaa tcgtgtcatc atcactttgt ttttaccact gatttgttca
     9901 ggtgtggctt tttactctct ctacatcaat gtcggagatg tattctttta ctggtatatg
     9961 ccagcgggtt attttagcgc ggttttcctg accggttatc tcactggcat ggggtatatt
    10021 tttctgccaa agtttaccca aaccgggcag caacgttatg cccacggtga agctatcgtt
    10081 aactatcttg cgcgtaaaga ggcagcaaca cacagtgggc ggcggaggaa aggggaaaca
    10141 cggaaactgg attacgcgtt gttaggttgg gctatatcgg caaatttggg gagggaatgg
    10201 gcgttacgca tcgccccttc gctttcttcg gcgatttgcg ctccagagat tgcccgtaac
    10261 ggcgttttat tctcattaca gacgcacctg agttgcgggg tctataccag tttgttgggg
    10321 cggagctatt ctggtggtgg ttctggcgct ggtggcggag gcggtggtgg ctggtaatca
    10381 agctgatgtt aatcgaaaca agctttaacc ataaagatag tgtagtatgt tgcgcctcaa
    10441 agccaggcaa gttaaaacgt cgagtcgttt acttaaggcc tgaagagttc aaacagactt
    10501 tactatcagt gagtcagatg taagttctga ctcattgttg cacaaaagca tatcagacgc
    10561 ctgggaaggc gacaccaaaa ggaagctcta tgagcgaaaa gctacagaaa gtgctggcgc
    10621 gtgccggcca cggttctcgc cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg
    10681 ttgatggcaa aattgctaaa ctcggcgatc gcgttgaaat tacccctgga ctgaaaatcc
    10741 gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt cgcgttctgg
    10801 cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg cgtccaacgg
    10861 tgtttgaccg tctgccaaaa ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg
    10921 ttaacacctg tggtctgttg ctgttcacca ccgacggtga actggcaaac cgtttaatgc
    10981 atccaagccg tgaagttgaa cgtgaatatg ccgtgcgtgt atttggtcag gttgacgacg
    11041 cgaaactgcg tgatttgagt cgtggcgtgc agctggaaga tggtccggca gcctttaaaa
    11101 ccatcaactt cagcggcggc gaagggatca accagtggta caatgttact ctgaccgaag
    11161 gccgtaaccg tgaagttcgt cgtctttggg aagcggttgg tgtgcaggtg agccgcctga
    11221 ttcgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt ggctggacgg
    11281 agctggacct cgcccagact aactatctgc gcgaactggt ggaactaccg ccagaaacca
    11341 gctctaaagt cgctgtagaa aaagaccgtc gtcgtatgaa ggcgaatcaa attcgtcgtg
    11401 cggtgaaacg tcacagccag gtgagcggcg gtcgtcgttc tggtggacgt aataataacg
    11461 gttaatcaaa gtatgccgga cgtcatatcc ggcattttta cagattagta atcaatccct
    11521 atctgcgctt ttacaccggc atcaaacgca tgtttgatgg ggcgcagctc acttaccgta
    11581 tccgccagtt caagaatatc ccgatgacaa ccacgacctg tgataatcac cgtctgttga
    11641 tgtggacgtt catttaacgc ctgaatcacg tcttccagtg gcaaataatc atacgctacc
    11701 atatatgtca gttcatcgag caaaaccata tccagtgagg aatcagccag catccgcttt
    11761 gcatgttgcc agacttcgcg gcaggcggcg gtatcagact ctcggttttg tgtatcccag
    11821 gtaaagcccg ttgccattac ctgaaactca acgccgtgtg gctccagcag atttcgttca
    11881 ccattaggcc aggtgccttt aataaactgc acgacgccca ctttttttcc gtgaccaact
    11941 gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt ttccattgcc ggtaaagacg
    12001 atgataatac cgcgttcatc ctgggcctgg gccacacgag catctacttt ttctttcact
    12061 cgctgctgac gctgttggta gcgttcatca ctcattggga aattcctggt ttacggcccg
    12121 gttgggcgtc aaaggtcatg ccggttttac ggcggctgtc atcgcccatc agccagaggt
    12181 agagcggcat gatatcagcg ggggttttaa gtttctgtgg atcttcggtc gggaaggcgc
    12241 tggcccgcat tgcggtgcgc gtaccgcctg ggttaatgca gttgacgcgc aggcgctgct
    12301 gatattcatc ggccagtacc tgcatcatcc cttcggtggc aaatttcgac gctgcatatg
    12361 cgccccagtt tgcacgcccc tgacgtccaa cgcttgatga ggtaaagacc agtgaaccgg
    12421 catccgattt gagtaataaa ggaagcagtg cctgtgtgag cataaaggtg gcattaacgt
    12481 tgacctgcgt gacgtcctgc cagacctgcg gatcttgttc gctcattggg caaacatcgc
    12541 cgagcaatcc ggcattatgc aaaacaccat ccagacgcgg ataattaacg gcaatgcgct
    12601 gtgccagttg ttggcaatct tcggacgtgc aggttagcaa atcgagaata aaccactgtg
    12661 gctgacgtcc aatttcttcg tttatgtggc tggctacctg acgtaatttt tcttcattac
    12721 ggcccaaaag aatcactgtc gcaccatagc gtgcatacgt catcgctgct tcacgaccaa
    12781 ttccatcgct ggctcccgtc accaggataa tgcgatcatt gagtaaatct tgttttggct
    12841 ggtaatgcat ggctactcct caacgacgtt gtctgtcgcg ctgccagtgt actttatgac
    12901 tttggggctt tatgcctgaa acagaagggc atttcaatca gcctggggcg ataacaccgc
    12961 aaaatggata ctttgtcata ctttcgctgc aataacatct ctgcgagacg gctaaacatg
    13021 cctgttgtaa actgtgagcc aaagcgttgt ttaaccaagg tggggactcg tggaattgtt
    13081 gtctgaatat ggtttgtttt tggcgaaaat cgttaccgtt gtgctagcga ttgcggcgat
    13141 tgccgccatt attgtcaatg ttgctcaacg taataaacgc cagcgtggcg agttacgagt
    13201 caacaatctc agcgaacagt ataaggagat gaaagaagaa ctggccgcgg cgctgatgga
    13261 cactcatcag caaaaacagt ggcacaaagc gcagaagaaa aagcacaagc aagaagcgaa
    13321 agccgcaaaa gcgaaagcta aactgggcga ggtggtaact gacagtaaac cccgcgtctg
    13381 ggtgctggat tttaaaggca gcatggacgc ccatgaagtg aactcgctac gtgaagagat
    13441 aacggcggta ctcgcagcat tcaaaccgca ggatcaggtt gtgctccgtc tggaaagccc
    13501 aggtggcatg gtgcatggtt acgggttggc ggcttcgcag ttgcagcgtc tgcgtgataa
    13561 aaacattcct ttaactgtta cggtagacaa agtcgctgcc agcggcggtt acatgatggc
    13621 ctgtgtggcg gacaaaattg tttccgcacc gtttgctatt gtgggttcca ttggggtggt
    13681 ggcgcaaatg cccaacttta accgcttcct gaaaagcaaa gatattgata tcgaactgca
    13741 caccgccggg cagtataagc gcacgctgac gttgctgggt gaaaataccg aagaagggcg
    13801 ggagaaattc cgcgaagagt tgaacgaaac gcatcagtta tttaaagatt ttgtgaagcg
    13861 tatgcgtccg tctctggata ttgaacaggt ggcaacgggt gaacactggt acggacaaca
    13921 ggcagtagag aaaggcctgg ttgatgaaat caacaccagt gatgaagtta ttcttagcct
    13981 gatggaaggc cgggaagtgg tcaatgtacg ctatatgcag cgtaaacgac tcattgaccg
    14041 attcaccggc agcgcggcag agagcgccga tcgattgttg ttacgctggt ggcagcgtgg
    14101 gcaaaagtcg ttgatgtaaa agacaaacgc gaggataaga cctcgcgttt tgctttaatc
    14161 aaccaagtga tatttttctg aaagcacatg ggccaggtgt ttgaacatat taaacaccgc
    14221 ggtgcttttg gcagttggta atccttgttc atctaaaaag tagtcgccgg taaataccag
    14281 cacgccatta cgctgcgtga cgccggtggc ttcaatccct gcgagcgtat cctcatgctc
    14341 acgaatgatt ttgttggcct ctttcaacag cgtttcgcgg tcgatgggtt gagtctcttt
    14401 gttcattatt tactccttaa acaaggacat tagtctacgc caggcatggc ttgcagacaa
    14461 atataccacg ctggtggcaa gagcgcctta ctggcaactt tggattttgc atgctaataa
    14521 agttgcgtat cggattttat caggtacagt gtgatgcttt cgtcaatctg gcaatagatt
    14581 tgcttgacat tcgaccaaaa ttccgtcgtg ctatagcgcc tgtaggccaa gacctgttaa
    14641 ctcagtcacc tgaattttcg tgagcagagt cacgacaagg ggttgatatc cgcagagagc
    14701 gagtccatat cggtaactcg ttgccagtgg aaggtttatc aacgtgcgac gcattcctgg
    14761 aagaatcaaa ttaggtaagg tgaatatggg taaagctctt gtcatcgttg agtccccggc
    14821 aaaagccaaa acgatcaaca agtatctggg tagtgactac gtggtgaaat ccagcgtcgg
    14881 tcacatccgc gatttgccga ccagtggctc aactgccaaa aagagtgccg actctacctc
    14941 caccaagacg gctaaaaagc ctaaaaagga tgaacgtggc gctctcgtca accgtatggg
    15001 ggttgacccg tggcacaatt gggaggcgca ctatgaagtg ttgcctggta aagagaaggt
    15061 cgtctctgaa ctgaaacaac tggctgaaaa agccgaccac atctatctcg caaccgacct
    15121 tgaccgcgaa ggggaagcca ttgcatggca cctgcgggaa gtgattggtg gtgatgatgc
    15181 gcgctatagc cgagtggtgt ttaacgaaat tactaaaaac gcgatccgcc aggcatttaa
    15241 caaaccgggt gagctgaata ttgatcgtgt taatgcccag caggcgcgtc gctttatgga
    15301 ccgcgtggtg gggtatatgg tttcgccgct tctatggaaa aagatcgctc gtggtctgtc
    15361 tgccggtcgt gtgcagtcgg tggcggtccg cctggtggtc gagcgtgagc gtgaaattaa
    15421 agcgttcgta ccggaagagt tctgggaagt cgatgccagc acgaccacgc catctggtga
    15481 agcgttggcg ttgcaggtga ctcatcagaa cgacaaaccg ttccgtccgg tcaacaaaga
    15541 acaaactcag gcagcggtaa gtctgctgga aaaagcgcgc tacagcgtgc tggaacgtga
    15601 agacaaaccg acaaccagta aacctggcgc tccttttatt acctctacgc tgcaacaagc
    15661 tgccagcacc cgtcttggtt ttggcgtgaa aaaaaccatg atgatggcgc agcgtttgta
    15721 tgaagcaggc tatatcactt acatgcgtac cgactccacc aacctgagtc aggacgcggt
    15781 aaatatggtt cgcggttata tcagcgataa ttttggtaag aaatatctgc cggaaagtcc
    15841 gaatcagtac gccagcaaag aaaactcaca ggaagcgcac gaagcgattc gtccttctga
    15901 cgtcaatgtg atggcggaat cgttgaagga tatggaagca gatgcgcaga aactgtacca
    15961 gttaatctgg cgtcagttcg tagcctgcca gatgacccca gcgaaatatg actccacgac
    16021 gctgaccgtt ggtgcgggcg atttccgcct gaaagcacgc ggtcgtattt tgcgctttga
    16081 tggctggacg aaagtgatgc ctgcactgcg taaaggcgat gaagatcgta tcttaccggc
    16141 agtcgataaa ggcgatgctc tgacgctcgt tgaactgaca ccagcccagc actttaccaa
    16201 gcctccagcc cgtttcagtg aagcatcgct ggtcaaagag ctggaaaaac gcggtatcgg
    16261 tcgtccttct acctatgcgt cgatcatttc gactattcag gatcgtggct atgtgcgagt
    16321 agaaaatcgt cgtttctatg cggaaaaaat gggcgaaatc gtcaccgatc gcctggaaga
    16381 gaatttccgc gagttaatga actacgactt caccgcgcag atggaaaaca gcctcgacca
    16441 ggtggcaaat cacgaagcag agtggaaagc tgtactggat cacttcttct cggatttcac
    16501 ccagcaatta gataaagctg aaaaagatcc ggaagagggt ggtatgcgcc cgaaccagat
    16561 ggttctgacc agcattgact gtccgacttg tggtcgcaaa atggggattc gcacagcgag
    16621 caccggggta ttccttggct gttctggcta tgcgctgccg ccgaaagagc gttgcaaaac
    16681 caccattaac ctggtgccgg aaaacgaagt gctgaacgtg ctggaaggtg aagacgctga
    16741 aaccaatgcg ttgcgcgcaa aacgtcgttg cccgaaatgc ggcacggcga tggacagcta
    16801 tctcatcgat ccgaaacgta agttgcatgt ctgtggtaat aacccaacct gcgacggtta
    16861 cgagatcgaa gagggtgaat tccgcattaa aggttatgac ggcccgatcg ttgagtgtga
    16921 aaaatgtggc tctgaaatgc acctgaaaat ggggcgtttc ggtaaataca tggcctgcac
    16981 caacgaagag tgtaaaaaca cacgtaagat tttacgtaac ggcgaagtgg caccaccgaa
    17041 agaagatccg gtgccattac cggagctgcc gtgcgaaaaa tcagatgcct atttcgtgtt
    17101 gcgtgacggt gctgccggtg tgttcctggc ggccaatacc ttcccgaaat cgcgtgaaac
    17161 gcgtgcgccg ctggtggagg agctttatcg cttccgcgac cgtctgccgg aaaaactgcg
    17221 ttatctggcc gatgcgccac agcaggatcc ggaaggtaat aagaccatgg ttcgctttag
    17281 ccgtaaaacc aaacagcaat atgtctcttc ggaaaaagac ggaaaggcga ctggctggtc
    17341 agcattttat gttgatggca aatgggttga agggaaaaaa taacctttaa ttctgcctgg
    17401 tttttataaa caaagggtcg cgaaagcggc ccttttttta cttagtagag ataaacggaa
    17461 ctgtgctgcc ggggatcatg gtatcgggca gtttaccgtc ccaacgctca gcagtggtga
    17521 gggcaacaag tccgggattc tcccgcagag cttcaccgcg caggcggata gcttctgctt
    17581 cagccgcact ttttaaacga atagtttcag cctctgccgc gcctcttacg cgaatagttt
    17641 ccgcttctgc tttggctgcc gctagtttac tgtctgcttc cgcctgggcc tgagttaccg
    17701 ctatttgcgc ctggattttc tccgtttcca gattttgctt acgcgtggca atggcgactt
    17761 ctgctttcat tcggtcttca atcgactttt cataagcgtc agaaaaatca atattttcaa
    17821 tctggacccc gtcaattacc actggcccaa caacggcttt gcgcatggca ttttgcagat
    17881 cctgaaccag ttttgtgcga tcctgtacgg cgctgatggc ggtatattgt ccgaaaacgt
    17941 tctccagttg agttggcaac tgacggacaa tcagacgatc ttttaatgct tcaatggtgt
    18001 tgtaggtggt ataaaccgcg cctgcttctg aaggtttaat gtggaagctg actgatactg
    18061 tcatttgtgc cggttgctgg tcgcggctgt aggcctgtaa tccctgatag accacagcct
    18121 gattacgcgt ggaaattttc tctacgcttt ccataaaagg gatcttaaaa cccagccccg
    18181 gctcggcaac ttttacgatt tttccgtagc gcagcaaaat gccgcgttca ccttcattaa
    18241 cggtgtaata cgagagaaat ggcagcacaa ccacggcaag cacgccaatg gcgatagcaa
    18301 gagatttttg tgggcgaaat gaggttaatg aaacaggtgc tttcatatga atcttccttg
    18361 agtaaagtgg ggaatatgtc acatgaggta aggagaaaat agccgacagg tgagaattca
    18421 gaatctataa aggggtatag cgaaaaaacg ttgcattaaa aaagtatgtt ttcggacatt
    18481 caata
//
LOCUS       DAZFRG010000086        18394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFRG010000086 DAZFRG010000000
VERSION     DAZFRG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.086"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..628)
                     /gene="rsmG"
                     /locus_tag="ACHKUC_002300"
     CDS             complement(5..628)
                     /gene="rsmG"
                     /locus_tag="ACHKUC_002300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO6618550.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(692..2581)
                     /gene="mnmG"
                     /locus_tag="ACHKUC_002301"
     CDS             complement(692..2581)
                     /gene="mnmG"
                     /locus_tag="ACHKUC_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO6618551.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2960..3403)
                     /gene="mioC"
                     /locus_tag="ACHKUC_002302"
     CDS             complement(2960..3403)
                     /gene="mioC"
                     /locus_tag="ACHKUC_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO6618552.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3493..3951)
                     /gene="asnC"
                     /locus_tag="ACHKUC_002303"
     CDS             complement(3493..3951)
                     /gene="asnC"
                     /locus_tag="ACHKUC_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO6618553.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4103..5095
                     /gene="asnA"
                     /locus_tag="ACHKUC_002304"
     CDS             4103..5095
                     /gene="asnA"
                     /locus_tag="ACHKUC_002304"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO6618554.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWVGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5100..6551)
                     /gene="viaA"
                     /locus_tag="ACHKUC_002305"
     CDS             complement(5100..6551)
                     /gene="viaA"
                     /locus_tag="ACHKUC_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO6618555.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6545..8041)
                     /gene="ravA"
                     /locus_tag="ACHKUC_002306"
     CDS             complement(6545..8041)
                     /gene="ravA"
                     /locus_tag="ACHKUC_002306"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO6618556.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8264..10132
                     /gene="kup"
                     /locus_tag="ACHKUC_002307"
     CDS             8264..10132
                     /gene="kup"
                     /locus_tag="ACHKUC_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO6618557.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIVNPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDISLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10299..10718
                     /gene="rbsD"
                     /locus_tag="ACHKUC_002308"
     CDS             10299..10718
                     /gene="rbsD"
                     /locus_tag="ACHKUC_002308"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO6618558.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10726..12231
                     /gene="rbsA"
                     /locus_tag="ACHKUC_002309"
     CDS             10726..12231
                     /gene="rbsA"
                     /locus_tag="ACHKUC_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO6618559.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12236..13201
                     /gene="rbsC"
                     /locus_tag="ACHKUC_002310"
     CDS             12236..13201
                     /gene="rbsC"
                     /locus_tag="ACHKUC_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6618560.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13226..14116
                     /gene="rbsB"
                     /locus_tag="ACHKUC_002311"
     CDS             13226..14116
                     /gene="rbsB"
                     /locus_tag="ACHKUC_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO6618561.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14242..15171
                     /gene="rbsK"
                     /locus_tag="ACHKUC_002312"
     CDS             14242..15171
                     /gene="rbsK"
                     /locus_tag="ACHKUC_002312"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6618562.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15175..16167
                     /gene="rbsR"
                     /locus_tag="ACHKUC_002313"
     CDS             15175..16167
                     /gene="rbsR"
                     /locus_tag="ACHKUC_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO6618563.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16133..17560)
                     /gene="mdtD"
                     /locus_tag="ACHKUC_002314"
     CDS             complement(16133..17560)
                     /gene="mdtD"
                     /locus_tag="ACHKUC_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO6618564.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17583..18275)
                     /gene="yieP"
                     /locus_tag="ACHKUC_002315"
     CDS             complement(17583..18275)
                     /gene="yieP"
                     /locus_tag="ACHKUC_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO6618565.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4420 a         4557 c         4811 g         4606 t
ORIGIN      
        1 aaaattaaat tttatttgct ttaatcacca ccagatgacg ttcgccatcc agggctggaa
       61 cctgaagttt aaccactgat tcgacctgat attcttcggg caacaaagcg atttcatctt
      121 ccggcatttg ccctttcagc gcgtagaaac ggccttgctc accaggaaga tggtggcacc
      181 agctcaccat atcgttcaga gaggcaaaag cgcggctaat tacgccatca aatggcggct
      241 ctgacggaaa ctcttctacc ctgctctgta ctggctcaat attctccagt ttaagctcat
      301 gttgcacctg acgaaggaaa cgcacgcgtt taccaaggct atccaacaac gtgaaatgag
      361 cttcaggacg cacgatagag agtggaatgc caggcagtcc cggtccggtg ccgacatcga
      421 taaaccgttc accttgcaga tacggtgcca ccacaatgct atcgagaata tggcgtacca
      481 gcatctcatt aggatcgcgg accgaagtca ggttgtacgc tttattccat ttatgcagca
      541 tattcacgta ggcaataagc tggtttttct ggtgatcggt aagcgaaata cctgcgtctt
      601 tcagcagtaa ggagagtttg ttgagcacgg tgattacctg ttcttgatgc gttgcctggt
      661 aagcgggtgc ttaccaggca tttttaatgc gttatgcgct acgacgcagc ataccctgtt
      721 ttttcagcca caccagcaga atggagatag ccgcaggcgt gacgccagaa atacgcgaag
      781 cctggccaat agaggccggt ttgtgatcgt taagtttggc gatcacttcg ttagaaagac
      841 cggatacctg gcggtaatcc agcgtcgctg gtagcagggt attctcgtta cgcagctgct
      901 tttcgatctc atcttgctgg cgcgcgatat aaccttcgta tttaacctga atctcaacct
      961 gttccgcagc ctgttcatct gtcaacgcag gggcaaacgg tgtcagcgtg gttaattttt
     1021 cataagtcat ttcaggacga cgtagcagat cttcaccact ggcttcacgg gaaagcggcg
     1081 cagtcaggtg agcattcact tcggctgcag cttccgccga cggggttacc caggtcgatt
     1141 tcagacgctg acgctcacgc tcgatattct caagtttctc gttaaagcgc gcccaacgtt
     1201 cgtcatccac caggcccagt tcgcgaccga tttcagtcaa acgcagatca gcattatctt
     1261 cgcgcagcat cagacgatat tctgcgcgcg aggtaaacat acgatacggt tctttggttc
     1321 ctaaagtgca caggtcatca accagtacgc cgagatatgc ctgagaacgc gccggagccc
     1381 aaccttcttt gtcagcagac agacgggcag cgttaagacc agccagcaaa ccttgcgcag
     1441 cggcttcttc gtaaccggta gtgccgttaa tctgaccagc aaagaacagc ccctggataa
     1501 acttgctctc cagcgtcggt ttcaggtcgc gtggatcgaa gaagtcatac tcaatggcat
     1561 aacccggacg cacgatcttc gcgttttcca tcccctgcat ggagcggacg atttgcatct
     1621 gcacatcgaa cggcaggctg gtggagatac cgttcggata aatttcgtta gaggtcagcc
     1681 cttccggttc aaggaagatc tgatgctgat ttctgtcggc gaagcgcatg actttgtctt
     1741 cgatcgacgg gcagtagcgt gggccgacac cttcgatcac ccctgcgtac attgggctac
     1801 gatcgaggtt actgcggatc acatcatggg ttttctcgtt ggtatgagtg atataacacg
     1861 gcacctgctg gggatgctgg gacgcattgc ccataaacga gaatactggc attgggttat
     1921 cgccatgctg ttgcgccagt acgctaaagt cgatggttcg agcatcaata cgcggtggtg
     1981 tcccggtttt cagacgacca acgcgcagcg gcagttcacg caaacggcga gaaagcggaa
     2041 tggacggcgg atcaccagca cggccaccgc tgtaattatc cagaccgata tgaattttac
     2101 cgtcgaggaa cgtcccaacg gtgagcacga cagctttggc acggaacttc agtcccattt
     2161 gggtaacggc accgaccacg cgatcgtttt cgacaataag atcttcaact gcctgctgga
     2221 agatcatcag gttcggttgg ttctccagcg ccgtacgtac cgcctgacgg tagagcacac
     2281 gatctgcctg agctcgggta gcgcgaaccg ccggtccctt gcttgcgttt agtatcctaa
     2341 actggatacc cgcctgatcg atcgctttcg ccatcagacc gccgagtgca tccacttctt
     2401 ttaccagatg tcccttccca ataccgccga tcgccgggtt gcagctcatc tgccccagag
     2461 tgtcgatatt gtgtgtcaaa agcagagtct gttgacccat acgcgccgcg gccatcgcgg
     2521 cctcggtgcc tgcatgaccc ccgccaatga tgatgacgtc aaaaggatcc ggataaaaca
     2581 tggtgattgc ctcgcataac gcggtatgaa aatggattga agcccgggcc gtggattcta
     2641 ctcaactttg tcggcttgag aaagacctgg gatcctgggt attaaaaaga agatctattt
     2701 atttagagat ctgttctatt gtgatctctt attaggatcg cactgccctg tggataacaa
     2761 ggatccggct tttaagatca acaacctgga aaggatcatt aactgtgaat gatcggtgat
     2821 cctggacagt ataagctggg atcagaatga ggggttatac acaactcaaa aactgaacaa
     2881 ccgttgttct ttggataact accggttgtt ccaagcttcc tgacagagtt atccacagtg
     2941 gatcgcacga tctgtatact tatttgagta aattaatcca cgatcccagc cattcttctg
     3001 ccggatcttc cggaatgtcg tgatcaagaa tgttgatctt cagtgtttcg cccgtctgtt
     3061 ttgcaccgga atttttgagt tcagcctcga gtttatcaat agccccacaa aaggtgtcat
     3121 attcacgact accaataccg attgcgccaa agcggaccgc agaaagatcg ggcttctgtt
     3181 cctgcaatgc ttcatagaaa ggagaaaggt tgtccggaat atctccggca ccgtgggtgg
     3241 agctgataac cagccagatc cccgaggcag ataaatcttc caacagcgga ccgtgcagcg
     3301 tttcggtggt aaaacccgcc tcttccagct tttcagccag gtgttctgct acatattcgg
     3361 caccgccgag ggtgctgccg ctgataagag tgatatctgc cataaaccgc cacctttatt
     3421 aagagtggcg tattgtacgc tgtgaaagcg ttgggatcta cctgtggaaa agtatgggat
     3481 taaaaaagcc gatcagggct taatggtacg catgatcggg ttctgcagga cgatcagtgt
     3541 ctcggtggac tgaatttcat caattgtttg gatcttgttg ataagtacat gctggagagc
     3601 atcgatcgaa cggcacatca cttttataaa gatgctgtag tggccggttg tgtaataggc
     3661 ttcagtgact tcatcgaggc tttccagctt tgccagcgcg gaaggatagt ctttggcgct
     3721 ctttaatata atgccgataa agcagcctac gtcataacca agctgcttcg ggctgacatc
     3781 aatacgcgcc ccggtgatga tccccgcctg cttcattttc tctactcgaa cgtgaatcgt
     3841 ccccggactg acgccaaatt gtttcgccag ttcggcgtaa gcggtgcgcg cattgcccat
     3901 taatgcttcc aggatgccac ggtccagatt gtcgattaga taattttcca taggattttc
     3961 ttatgcggat tgatgattca ttctatttta gccttctttt ttaatgaatc aaaagtacgt
     4021 gaggcttttt attgaatgat tattgcatgt gtgtcggttt ttgttgctta atcataagca
     4081 acaggacgca ggagtataaa aaatgaaaac cgcttacatt gccaaacaac gtcaaattag
     4141 cttcgtgaaa tctcactttt ctcgtcaact ggaagaacgt ctggggctga tcgaagtcca
     4201 ggcgccgatt cttagccgtg tgggggatgg cacgcaggat aacttgtcgg gctgtgaaaa
     4261 agcggtgcag gtaaaagtga aagctctgcc tgatgcccag tttgaagtgg ttcattcact
     4321 ggcgaagtgg aaacgtcaga ccttagggca acacgacttc agcgcgggcg aagggctgta
     4381 cacgcacatg aaagcccttc gccccgatga agaccgtctt tccccgctgc actcggtcta
     4441 tgtagaccag tgggactggg aacgcgtaat gggcgacggc gagcgtcaat tctcgactct
     4501 gaaaagcacg gtagaggcga tctgggtggg aattaaagca accgaagctg cggttagcga
     4561 agagtttggc ctggcaccgt tcctgccgga tcagatccat ttcgtacaca gccaggagtt
     4621 actgtctcgt tatccggatc tcgatgccaa agggcgtgag cgggcgattg cgaaagatct
     4681 tggcgcggta ttcctcgtcg ggattggcgg caagttgagt gatggtcatc gccacgacgt
     4741 gcgcgcaccg gattatgatg actggagcac cccgtcagag ctgggccatg cgggtctgaa
     4801 cggcgatatt ctggtatgga acccggtact ggaagatgcg tttgagcttt cttccatggg
     4861 gatccgcgtg gatgccgaca cgctgaagca tcagctggcg ctgaccggtg acgaagatcg
     4921 cctgcagctg gagtggcatc aggcgctgct gcgcggtgaa atgccgcaga ccatcggcgg
     4981 cggtatcggg cagtctcgtt tgaccatgct gctgctgcaa ctgccacata tcggccaggt
     5041 tcagtgtggt gtatggccgg cagctgttcg tgagagcgtc ccttctctgc tgtaataatt
     5101 tatcgccgcc agcgtctgag caggcggctt cgcatcccgg tatcaaagcg ccagatatga
     5161 tcgaaaatgc gcatgatgcc gggtttgcca tgtgccgaca tcgccacggc atgaaagcga
     5221 tgctgatgta cccgctgcaa ctctttcact ttactcgtca cgtcgtcagg caaccgctgg
     5281 gcgataaaat cagaaatcac caccgcatcg gcatcaaacc attccctgct ttgcaagcgt
     5341 tccataatgg cgcgaaaaca actggcaaga tcggtgccgc cacgaaactg ctgacttaaa
     5401 aagcggatcg cttgctcaat accttgtggc cctgaaagct cataacggac gatctcagtg
     5461 gaaaatagca taatatagca gcgccggttt tcagcgaggg ctatgcgcat caaggccagg
     5521 cagaacgctt tcgcacactg ttcattaaag ccgcccattg agccggaagt atccacgcag
     5581 acaataaacg gtccgcgcgg ctgttcgtcg taatctttat gcaccaccgg gcgttcgatc
     5641 actttttcac gccacgactc accatgcagg cgataggtga gcaactgttt ttccaccagc
     5701 cgacggtaaa actcatactc cagttccgtt atccctagtg tcgccagttc tggcggcaag
     5761 agacgtaaaa tatcatcgct ttgctgcaga ccatcaacct gctcaggaac tgtcgccggt
     5821 tcgcgcacca tggtgcggaa ggtttccatc tgcgcatcgt tgcgcggtat tgatttggct
     5881 tcccgggaac gccccagctg ttctgccaga cgtttcagtt ccggctgttc gttaagaaat
     5941 tcaccgtatt tcacaatcaa ctgatagtcg ccacgtttaa gctgaccggc actcatatcc
     6001 cacagacgac cagctgcggt attgttatct gcgagaatcg gttcaagttg cccgctcagc
     6061 gtcatgcgtt cctgaacttc actcaacagt tgttcgcgtt cttcttctaa tagctgctga
     6121 tttaacgtcg ttgcttgcac gatcaggctt aaacgccagc gctggagaaa aagcgtgtgt
     6181 aacgctgaag tgatcgtact gttagcatca accaactgtc gggcttgttc tgcccatggg
     6241 gaattcagac gatgcagtaa gtccaggatt tgtggtagct gcacaataaa ctgcggcgtg
     6301 gagaggagct ggctttgctg atagcacatc acctcttcgg tgagttccgg cgggactcgg
     6361 gcatctttca gccgactgcg cagcgcctca cgccagcggg gaacatcatc agtgattgcc
     6421 gccttcagtc gtgggaattt ttcaaagaag actgccagct gcggtgaggc cagcagcgcg
     6481 atgatcatct cttcgatcaa tccctcttcg ctgacggcca gcatcacatt aagcgtatcc
     6541 agcgttagca ttgttgtgcc tggcgaatct gtgcgccgac atcctgtagg ctggcttcaa
     6601 tgcgacctaa ccagtcgcca gggataaaca gacatttttg ctgttcgctg aataaagcgt
     6661 gttgcttgcg ccagtcgctt tcaagctctt ccagttgttg ttttatttcg ctgggcagac
     6721 cttcagccga tgaaccggga agtgctagcg tactgccttg taaactcaca tcgcgtacaa
     6781 ccagatgttg ggcgctatcg acttccagat tcagtttctg ggcaaagccg ataccgttca
     6841 gtttgccgcg aatctcacca cctttactca gccactgttc cagcgcgcta cgctcaaagg
     6901 agatatgaac cacttccata tcatgcagtt ttaacggttt ttgcagcagc agagtcagag
     6961 tggaagcagt aacgttaaca gggagttgat actgctgacg acggctaaaa atgccgccca
     7021 gacgaattac cgttaaggct gttttatcgc tttgttgctg ctgaagttgc aggtgacgtt
     7081 gcacaatcgc gcccaggcgg gtcaacatcc cttgctgttg ccaggcgtga ccggtcatca
     7141 atacatcaat ttgttgttgt atcaaattca ggctttgcgc gtcataccac aggcaatctt
     7201 tcagcaaaat gagatcaacc ggggcgacag cactgcgacc gctaaaaaag gcgctggcct
     7261 gcaataagcg gatcgctttt ttccagcgac gatccgagac ataaggcgca tccggtaatt
     7321 tatccagttg ctggcgcagc ataaaaatca gctcaaatac atgatcgggc agcgtaattt
     7381 caccaatctc tttctgccag cgttcatatt cttcatctgt gatctgcaag gaggcaggaa
     7441 ccggattgtc attttcatcc tgttgactgg tcagcatgga gcggaaattc gctttatcct
     7501 gcactttatc taaccacaga cgaatcagca tgcggtcata taacgcttcc agactgctgt
     7561 ctgcttccgg cagctcgttg gaggccgcca ccagcagacg catcgggatt ttttctacga
     7621 gtgcgccgtt gcggaactgg cgctcgttaa tggcggtgag cagggtatta agaattgccg
     7681 gacccgcttt ccagatctca tccagaaaga cgatttcagc ttccggcagg taaccgctgg
     7741 ttaaacgttc atagcgccct tcatctttta gcgcctgaat agaaagggga ccaaaaactt
     7801 cttccggcgt ggagaagcgg gtcatcagat attcaaacgc gcgggcattc tgaaaggcga
     7861 attttaagcg ccgggcgatc aaacttttgg caatacctgg tgggccaagg aggaacacac
     7921 tttcaccact taatgccgct aataaacaca agcggatggc gtggctacgt tcataaagcc
     7981 ccttttccaa cgaactgctc aggcgggaaa ttctttccgc taataaatga gggtgagcca
     8041 taatgaagtg gcgtcctttc atcaaaagtt ctgcgtaaat tgcgagtata gacgtttctt
     8101 gctggtggct aaaatagtct caaaggaggg gtatttttct ttgagccagg ttaatgtggc
     8161 cgcatttagg agtacgattt tgccgttaat cgtgcatact gtgcgctttt ttgtgggcca
     8221 agggactaag cacacatttc atatttcaac gaaagactag tctatgagca ctgataataa
     8281 gcaatcattg cccgcgatta ccctcgcggc gattggagtt gtctacggcg atattggtac
     8341 cagcccgtta tatacacttc gtgaatgttt gtccggccag tttggttttg gcgttgaacg
     8401 cgatgccgtg tttggctttt tatcgctgat cttctggctg ctaatctttg tggtttccat
     8461 taaatatctc accttcgtga tgcgggcaga taacgccggt gaagggggga tcctgacgtt
     8521 gatgtcgctt gccgggcgta atacgtcggc gcgaaccaca tcaatgctgg tgattatggg
     8581 gctaatcggc ggcagctttt tctatggtga agtcgtcatt actcccgcta tttcggtgat
     8641 gtccgccatt gaaggtctgg aaatcgtcgc cccgcagctg gatacctgga tagttcccct
     8701 ctcaattatc gttctcacat tattattcat gattcaaaaa catggcacgg ctatggtcgg
     8761 taagctgttt gcgccgatca tgctgacctg gtttttgatt ctggcagggc tggggttacg
     8821 tagcattatt gttaacccgg aagtgctgca tgcactgaat ccgatgtggg cggtgcattt
     8881 cttccttgaa tacaaaacgg tttcttttat tgcattaggg gcagtggtgc tgtcgattac
     8941 gggggtcgag gcgctgtatg ctgatatggg gcactttggt aagttcccta ttcgtctggc
     9001 gtggttcacc gtcgtattgc cttccttaac ccttaattac ttcggccagg gagcgctgtt
     9061 gttaaagaat ccggaagcga ttaagaaccc gttcttcctg ttggcaccgg actgggcgct
     9121 gatcccgctg ctgatcatcg ccgcactggc gacggtaatt gcctcgcagg cggttatctc
     9181 tggcgtcttc tcattgacgc gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat
     9241 tattcacact tccgaaatgg agtcagggca aatctatatt ccctttgtga actggatgct
     9301 ctatgtcgcg gtcgtgattg tgattgtcag ctttgagcac tccagcaacc tggcggcggc
     9361 gtacgggatt gcggtgaccg gaaccatggt gctgacgtct attctctcga ctaccgtggc
     9421 acgtcagaac tggcactgga ataagtattt tgttgcgctg atcctgattg ctttcctttg
     9481 tgtcgatatt tccttgttca ccgctaacct cgataaactg ctctccggcg gctggttgcc
     9541 attgagcctc ggtacggtga tgtttatcgt gatgaccacc tggaagagcg agcgtttccg
     9601 cttgctgcgg cggatgcatg aacatggtaa ctctctggaa gcgatgattg cttcgctgga
     9661 gaaatcaccg cccgttcgcg tgcctgggac cgcggtgtat atgtcgcgtg caatcaacgt
     9721 cattcccttt gcgctgatgc ataacctgaa acataacaag gtattgcatg agcgggtgat
     9781 tctgttaact ctgcgcaccg aagacgcgcc atatgtccat aacgtccgtc gggtacagat
     9841 tgaacaactg tcgcccactt tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc
     9901 aaacgtggaa gaagttttcc accgctgcgg tctggaagga ttaagttgcc ggatgatgga
     9961 aacctccttc tttatgtcgc atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg
    10021 tctgcgcggc aagctgtact tgctgctgca acgtaatgcg ctgcgggcac cggatcaatt
    10081 tgaaatcccg ccaaacagag ttatcgaact gggtactcag gtcgaaatct aacgccagac
    10141 gcctcctttc tccataaggg ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt
    10201 tcgaggttga tcacatttcc gtaacgtcac gatggttttc ccaactcagt caggattaaa
    10261 ctgcgggtca gcgaaacgtt tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta
    10321 attctgatat ttcatcggtg atctcccgac tgggacatac cgatacgctg gtggtgtgtg
    10381 atgctggttt acccatcccc aaaagtacaa cgcgtatcga tatggcatta acccagggtg
    10441 taccttcttt tatgcaggtg ctgggtgtag tcacaaatga aatgcaggtc gaggctgtca
    10501 ttatcgcgga agagatcaaa caacataatc cgcaactcca cgaaacgttg ctcactcacc
    10561 ttgagcagct gcaaaaacac cagggaaaca ccattgaaat tcgttacacc acgcatgaac
    10621 aattcaaaca acaaaccgca gaaagtcagg cggtaattcg cagcggagaa tgttctccgt
    10681 atgcgaatat cattctctgt gctggcgtga cgttctgagg ccgtcatgga agcattactt
    10741 cagcttaaag gcatcgataa agccttcccg ggcgtaaaag ccctctcggg cgcagcgtta
    10801 aatgtctatc cgggccgcgt gatggcgctg gtgggcgaaa acggcgcggg taaatccacc
    10861 atgatgaaag tgcttactgg tatctatact cgcgatgccg gtacgctttt atggttgggg
    10921 aaagaaacga catttaccgg gccgaaatct tcccaggaag ccgggattgg gattatccac
    10981 caggaactga acctgatccc gcagttaacc attgccgaaa acattttcct tggtcgtgag
    11041 tttgttaatc gctttggcaa aattgactgg aaaaccatgt atgccgaagc ggataaattg
    11101 ctggctaaac ttaacctgcg ctttaaaagc gacaagctgg tgggcgatct ttccatcggt
    11161 gaccagcaaa tggttgaaat cgccaaagtg ctgagctttg agtcgaaagt catcattatg
    11221 gatgaaccga ccgatgcgct gaccgatacc gaaaccgaat ccttgttccg cgtcatccgc
    11281 gagctgaaat cgcaaggccg tggtattgtc tatatctccc accgcatgaa agaaatcttc
    11341 gagatttgcg atgacgttac cgtttttcgt gacggacaat ttattgctga gcgcgaagtg
    11401 gcatcactga ccgaagattc tctgattgag atgatggtgg gccgcaagct ggaagatcaa
    11461 tatccgcacc tggacaaagc gccgggagat atccgcctga aagtcgataa tctctgcgga
    11521 cctggcgtta acgatgtctc ttttacttta cgcaaaggcg aaattcttgg cgtctctggt
    11581 ttgatgggcg ctgggcgtac cgaactgatg aaagtgctct acggtgcact gccgcgcaca
    11641 agcggttacg tcaccttgga tgggcatgaa gtcgttaccc gttcgccgca ggatggcctg
    11701 gcaaacggca ttgtttatat ctccgaagac cgtaaacgtg acggtttagt gctgggcatg
    11761 tcagtaaaag agaacatgtc gctgaccgcg ctgcgctact tcagccgtgc tggcggcagt
    11821 ttgaagcatg ccgatgaaca gcaggctgtg agtgatttca ttcgtctgtt taatgtgaaa
    11881 acgccatcga tggaacaggc aattggtctg ctttccggtg gcaatcagca aaaagtagcg
    11941 attgcccgtg gtctgatgac acgccctaaa gtgttgatcc tcgatgagcc tacccgtggc
    12001 gtagatgtcg gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc
    12061 ttgagcatca ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc
    12121 atcgtcatgc atgaagggca tctcagcggg gaatttactc gtgagcaggc cacccaggaa
    12181 gtgttaatgg ctgccgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgac
    12241 aacccagact gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc
    12301 gcttatcgct ctgctggtgc tgatcgcgat tgtctcgacg ttaagcccga actttttcac
    12361 catcaataac ttattcaata ttcttcagca aacctcggtg aacgccatta tggcggtcgg
    12421 gatgacgctg gtgatcctga cgtcgggtat cgacttgtcg gtaggttctc tgttggcgct
    12481 gaccggcgca gttgctgcat ctatcgtcgg cattgaagtc aatgcgctgg tggctgtcgc
    12541 tgccgctctc gcgttaggtg ccgcaattgg tgcggtaact ggggtgattg tagcgaaagg
    12601 tcgcgtccag gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat
    12661 ggtttatacc aatggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg
    12721 ctggtttggt attggtcgtc cgctgggcgt accgacgcca gtctggatca tggggattgt
    12781 cttcctcgcg gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct
    12841 gggcggcaac gaagcggcaa cgcgtctttc tggtatcaac gtcaataaaa tcaaaatcat
    12901 cgtctattct ctttgtggtc tgctggcatc gctggccggg atcattgaag tggcgcgtct
    12961 ctcctccgca caacccactg cggggactgg ctatgagctg gatgctattg ctgcggtggt
    13021 tctgggcggt acgagtctgg cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc
    13081 attaattctt ggcttcctta ataatggatt gaatttgtta ggtgtttcct cctattacca
    13141 gatgatcgtc aaagcggtgg tgattttgct ggcggtgctg gtagacaaca aaaagcagta
    13201 ataacgacta caggacatct tgaatatgaa catgaaaaaa ctggctaccc tggtttccgc
    13261 tgttgcgcta agcgccaccg tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt
    13321 ggtctccacg cttaacaacc cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc
    13381 ggataaactt ggctataacc tggtggtgct ggactcccag aacaacccgg cgaaagagct
    13441 ggcgaacgtg caggacttaa ccgttcgcgg taccaaaatt ctgctgatta acccgaccga
    13501 ctccgacgca gtaggtaatg ctgtaaagat ggctaaccag gcgaacatcc cggttatcac
    13561 tcttgaccgc caggcaacga aaggtgaagt ggtgagccac attgcatctg ataacgtact
    13621 gggcggcaaa atcgccggtg attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat
    13681 cgagctgcaa ggcattgccg gtacatccgc agcccgtgaa cgtggcgaag gtttccagca
    13741 ggccgttgct gctcacaagt ttaatgttct tgccagccag cctgcagatt ttgatcgcac
    13801 caaaggtttg aacgtaatgc agaacctgtt gaccgctcat ccggatgttc aggctgtatt
    13861 cgcgcagaat gatgaaatgg cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc
    13921 ggatgtgatg gtcgtcggat ttgacggtac accggatggc gaaaaagcgg tgaatgatgg
    13981 caaactagca gcgactatcg ctcagctacc cgatcagatt ggcgcgaaag gcgtcgaaac
    14041 cgcagataaa gtgctgaaag gcgagaaagt tcaggctaag tatccggttg atctgaaact
    14101 ggttgttaag cagtagtttt aatcaggtta catgacctga tggtgacata aatacgtcat
    14161 cgacagatga acgtgtaata taaagaaaag cagggcacgc gccaccctaa cacggtggcg
    14221 cattttatgg acatcccgaa tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt
    14281 aatgctgacc acattcttaa tcttcaatct tttcctactc caggcgaaac cgtaaccggt
    14341 aaccactatc aggttgcatt tggcggcaaa ggcgcgaatc aggcggtggc tgctgggcgt
    14401 agcggtgcga atatcgcgtt tattgcctgc actggcgatg acagcattgg tgagagcgtt
    14461 cgccagcagc tcgccactga taacatcgat atttctccgg tcagcgtgat caaaggcgaa
    14521 tcaacaggtg tggcgctgat ttttgttaat ggcgaaggtg agaatgtcat cggtattcat
    14581 gccggcgcta atgctgccct ttccccggcg ctggtggaag cgcaacgtga gcgtattgcc
    14641 aacgcgtcgg cattattaat gcagctggaa tcaccactcg aaagtgtgat ggcagcggcg
    14701 aaaatcgccc atcaaaataa gactatcgtt gcgcttaacc cggctccggc tcgcgaactt
    14761 cctgacgaac ttctggcgct ggtggacatt attacgccaa acgaaacgga agcagaaaag
    14821 ctcaccggta ttcgtgttga aaatgatgaa gatgcagcga aggcggcgca ggtactgcat
    14881 gaaaaaggta tccgtactgt actgattact ttaggaagtc gtggtgtatg ggctagcgtg
    14941 aatggtgaag gtcagcgcgt tcctggattc cgggtgcagg ctgtcgatac cattgctgcc
    15001 ggagatacct ttaacggcgc gttaatcacg gcgttgctgg aagaaaaaac attgccagag
    15061 gcgattcgtt ttgcccatgc agccgctgcg attgccgtaa cacgtaaagg cgcacaacct
    15121 tccgtaccgt ggcgtgaaga gatcgacgca tttttagaca ggcagaggtg acgcttggct
    15181 acaatgaaag atgttgcccg cctggcgggc gtttctacct caacagtttc tcacgttatc
    15241 aataaagatc gcttcgtcag tgaagcgatt acagccaaag ttgaagcggc gattaaagaa
    15301 ctcaattacg cgccatcagc tctggcgcgt agcctcaaac tcaatcaaac acataccatt
    15361 ggcatgttga tcactgccag taccaatcct ttctattcag aactggtgcg tggcgttgaa
    15421 cgcagctgct tcgaacgcgg ttatagtctt gtcctttgca ataccgaagg cgatgaacag
    15481 cggatgaatc gtaatctgga aacgctgatg caaaaacgcg ttgatggctt gctgttactg
    15541 tgcaccgaaa cgcatcagcc ttcgcgtgaa atcatgcaac gttatccgac agtgcctacc
    15601 gtgatgatgg actgggctcc gttcgatggc gacagcgatc ttattcagga taactcgttg
    15661 ctgggcggag acttagcaac gcaatatctg atcgataaag gtcatacccg tattgcctgt
    15721 attaccggtc cgctggataa aactccggcg cgcctgcggt tggaaggtta tcgggcggcg
    15781 atgaaacgtg cgggtctcaa cattcctgat ggctatgaag tcactggtga ttttgaattt
    15841 aacggcgggt ttgacgctat gcgccaactg ttatcacatc cgctgcgtcc tcaggccgtc
    15901 tttaccggaa atgacgctat ggctgttggc gtttaccagg cgttatatca ggcagagtta
    15961 caggttccgc aggatatcgc ggtgattggc tatgacgata tcgaactggc aagctttatg
    16021 acgccaccat taacaactat acaccaaccg aaagatgaac tgggggagct ggcgattgat
    16081 gtactcatcc atcggataac ccagccgacc ctacagcaac aacgattaca acttactccg
    16141 attctgatgg aacgtggttc ggcttagatt tacgctgtct tttgatcaaa ttattaccat
    16201 cggttgtttt cagtagcatg aacattgctg ctgaagcaac agtaataatg cccatcgtga
    16261 taaacgtata gtggaattgt tcgactgtcg ttgtgccttc catcccttcg taaacgcgaa
    16321 ggactgccgc acttacagca acgcctaaac tgatcgacag ttgctgcgtg accgccagaa
    16381 cactgttacc gctgctggcg ttgtcatcgg tcagatcggc aagtgtgatg gtattcatcg
    16441 cggtaaattg tgtcgacata gccatcccta atataaacaa cggcaagatc agcatccata
    16501 tcgccattgc tggtgattgc aaagagaact gagcgatcat tagccctata atcaccgtga
    16561 tccccactaa cgtatggcga tagcccagac gacgtaagac ttgggtaacc atcgattttg
    16621 caataatgga acctaacgct gtcggtgcca tcatacagcc agcaataaac gcctgatacc
    16681 caaatcctac ctgtaacatc aatggcataa ggaacggtac acagccggtc cccagacggg
    16741 ttgcaatatt gcctacgata ccgatcgaga aagtgcgggt tttaaataaa tctaatgaaa
    16801 ttaatgggtt tggcgtgcgt cgcgcatgga gaatatagag aagcagtaac ccgatgctgg
    16861 tgacaattac cgtcaaggca atccagctgg cgacaatctt ttccccgaat agctctattc
    16921 cgcttgagaa gagaacaagg ctgaggccaa acagcaaaaa gccagtgata tcgaatctgc
    16981 gtcgtgcggt ggtgaaattg ggcatatgtt tgcgcgcgta aagaaggccc gcaataccta
    17041 tggggatatt gattaaaaat atccagtgcc aggttgccca ggtgaccagc acgccgccaa
    17101 gaacggggcc taaaattggc cccaccagac ccggcatggc gacaaaattc aatacaggaa
    17161 gaagttcatt acgaggataa gcgcgcagta aggccagccg agcaacaggc atcatcattg
    17221 cgccgcctat cccctgaata acccggaaga caaccagctg tggtagcgag ttggaaagtg
    17281 cgcaggccag agaacccaac gtgaacagac tcacggcaag ggtaaaaatg cgacgtgtac
    17341 cgaagcgatc ggctagccat ccgcttaccg gaataagcat cgccaccgtc agcgtataac
    17401 tgatgatggc tgattgcatc gcgagaggag aacgattaag gctatgagcg attgcgggta
    17461 aggcggtatt cagaatagtg gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc
    17521 acggcaaacc cgccatactg cgcttctttt tatcgctcat tcaatgtcct gttatcgggt
    17581 tatcacttat ctggtgagcg tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg
    17641 ctttggataa tcgcatcgac aatcgcttgg tgcagatcca gctttatcac tgtgtcgctg
    17701 gtaattgacg tgaagtaagt gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa
    17761 aacggattgg cgctcatttc atagatatgc tcatgccagg ccatatcgac ttcgatccag
    17821 cgttcacggc gaaagttctc ttttaatgcc gccatttcgg ccattaacgt attgagatgc
    17881 gccttctgtt ccgcggtgcc aacctttgct gccagtaggc aagcttgcgg ctccagacag
    17941 atacgcataa ccagaaagtg atcgatgacc tgatgaaagt tctcttctgt catccaccag
    18001 gtaagcaatt cctgatcaag aaaattccag ttcgattgtg gcatgactcg ggtaccaatt
    18061 cgcggtcgcg gtaaaaccat cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct
    18121 gtacgactca ctccaaattg ctcgcccagc tcaatctcac caggcaaaat ggtgccgggt
    18181 tcatattcac cttttaagat ccgctgcgcc agcttctcag ccagaacata cgaaaggttt
    18241 ttctgtgcag ctaactgttg tgcgcttaaa ggcattacta atcttccttt ttctttttat
    18301 tccttcttag tatgccacca ggaagtgtga ttacggttgc aaaaacggca aattgcttgt
    18361 tttatggcac attaacgggg cttttgctga aaaa
//
LOCUS       DAZFRG010000087        18384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFRG010000087 DAZFRG010000000
VERSION     DAZFRG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.087"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..921
                     /gene="yfbL"
                     /locus_tag="ACHKUC_002316"
     CDS             4..921
                     /gene="yfbL"
                     /locus_tag="ACHKUC_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO6618566.1"
                     /translation="MIFYQPWVRALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDN
                     LNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVANYGPADGPLIIIGAHYDSVSSY
                     ENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLVAYASEEPPFFRSDEMGSA
                     VHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINA
                     VRQVKAALLSSRDLSVYSMNAPGFIPGVDFSDHLNYWQHDIPAVMITDTAFYRNKQYH
                     LPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1024..1527
                     /gene="yfbM"
                     /locus_tag="ACHKUC_002317"
     CDS             1024..1527
                     /gene="yfbM"
                     /locus_tag="ACHKUC_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO6618567.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1594..3051)
                     /gene="nuoN"
                     /locus_tag="ACHKUC_002318"
     CDS             complement(1594..3051)
                     /gene="nuoN"
                     /locus_tag="ACHKUC_002318"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO6618568.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SASGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3058..4587)
                     /gene="nuoM"
                     /locus_tag="ACHKUC_002319"
     CDS             complement(3058..4587)
                     /gene="nuoM"
                     /locus_tag="ACHKUC_002319"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO6618569.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4751..6592)
                     /gene="nuoL"
                     /locus_tag="ACHKUC_002320"
     CDS             complement(4751..6592)
                     /gene="nuoL"
                     /locus_tag="ACHKUC_002320"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO6618570.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6589..6891)
                     /gene="nuoK"
                     /locus_tag="ACHKUC_002321"
     CDS             complement(6589..6891)
                     /gene="nuoK"
                     /locus_tag="ACHKUC_002321"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO6618571.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6888..7442)
                     /gene="nuoJ"
                     /locus_tag="ACHKUC_002322"
     CDS             complement(6888..7442)
                     /gene="nuoJ"
                     /locus_tag="ACHKUC_002322"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO6618572.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7454..7996)
                     /gene="nuoI"
                     /locus_tag="ACHKUC_002323"
     CDS             complement(7454..7996)
                     /gene="nuoI"
                     /locus_tag="ACHKUC_002323"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO6618573.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8011..8988)
                     /gene="nuoH"
                     /locus_tag="ACHKUC_002324"
     CDS             complement(8011..8988)
                     /gene="nuoH"
                     /locus_tag="ACHKUC_002324"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO6618574.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8985..11711)
                     /gene="nuoG"
                     /locus_tag="ACHKUC_002325"
     CDS             complement(8985..11711)
                     /gene="nuoG"
                     /locus_tag="ACHKUC_002325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO6618575.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIIGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSQTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11764..13101)
                     /gene="nuoF"
                     /locus_tag="ACHKUC_002326"
     CDS             complement(11764..13101)
                     /gene="nuoF"
                     /locus_tag="ACHKUC_002326"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO6618576.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13098..13598)
                     /gene="nuoE"
                     /locus_tag="ACHKUC_002327"
     CDS             complement(13098..13598)
                     /gene="nuoE"
                     /locus_tag="ACHKUC_002327"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO6618577.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13601..15403)
                     /gene="nuoC"
                     /locus_tag="ACHKUC_002328"
     CDS             complement(13601..15403)
                     /gene="nuoC"
                     /locus_tag="ACHKUC_002328"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO6618578.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15497..16159)
                     /gene="nuoB"
                     /locus_tag="ACHKUC_002329"
     CDS             complement(15497..16159)
                     /gene="nuoB"
                     /locus_tag="ACHKUC_002329"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO6618579.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16175..16618)
                     /gene="nuoA"
                     /locus_tag="ACHKUC_002330"
     CDS             complement(16175..16618)
                     /gene="nuoA"
                     /locus_tag="ACHKUC_002330"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO6618580.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17247..18185)
                     /gene="lrhA"
                     /locus_tag="ACHKUC_002331"
     CDS             complement(17247..18185)
                     /gene="lrhA"
                     /locus_tag="ACHKUC_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO6618581.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4591 a         5099 c         4754 g         3940 t
ORIGIN      
        1 tttattattt tttaccaacc ctgggtcagg gcattaccac caacgccccg acacgctagt
       61 cctgagcaat tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtagcgcc
      121 gacaatattg ataatctgaa taggtccgca gagtatataa aagaggtctt tatcagtagc
      181 ggtgccagag ttaccgcgca ggatgtcccc attacgggtg gcccctacaa aaacattgtt
      241 gctaattatg gtcctgccga tggaccgctg attattattg gtgcgcacta tgactctgtc
      301 agtagttatg aaaacgatca attgacctat acgccgggcg cggatgataa cgccagcggc
      361 gtggcaggat tactcgaact ggcacgtttg ttacagcagc agactccagg gattggcgtc
      421 caactggtcg cctatgcgtc ggaagaaccg cccttctttc gtagcgatga aatggggagc
      481 gcggtacatg cagcttcgct tgagcgtcca gtgaaattaa tgatagcgct ggagatgatt
      541 ggttattacg actcaacgcc tggtagccag gattacccat ccccggcaat gtcctggctt
      601 tatcccgatc ggggagactt tattgccgtg gtcggcagaa tgcaggatat caacgccgtt
      661 cgtcaggtaa aagcggcatt gttatcatcc cgggatttat ctgtttattc tatgaatgcc
      721 ccaggtttta ttcccggtgt tgatttctct gatcacctta attattggca acacgatatt
      781 cccgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt
      841 gatatcgcag acagattaaa ttatcagaaa atggctcagg tagtggatgg agttacaacc
      901 ttgttataca acagtaaata ataaaaattt ctcgaggcgg atccagaaaa agcaacatgc
      961 gcattttatt ttctttcgtc tgttttaaga tgcgaaacat gaaattcaac ggaggaaaaa
     1021 taaatgggaa tgattggcta ttttgcagaa attgactcag aaaagattaa ccaactgctt
     1081 gagtctacta aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga
     1141 ctggatattg ataaacgctg ggatttttta cactttgggc tgacaggcac ctctgctttt
     1201 gatcccgcga agaatgaccc gctgagtcgc gccgtgcttg gtgaacacag cctagaagat
     1261 ggcatagatg gcttcctggg attgacctgg aatcaggagt tagccgcgac aatcgaccga
     1321 ctggaatcac tggatcgaag tgagctgcgt aaacagtttt ccattaagcg actcaatgaa
     1381 atggagattt atccgggagt aaccttttca gaagagttag aggggcaact ctttgcttcg
     1441 atcatgcttg atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat
     1501 catgcgctga cggttattgt tgggtgatgg tgtcagggca ccaaaaaaaa ccgccgaaca
     1561 tgtcggcggc tttctgactt acaaactgac tgtttacatc agcggcattg ctaaccgcac
     1621 aatgctaatc agcggttgtg gccatacacc cagcaccagt accaacagtg cagagatcag
     1681 caccacgata ccgcttgcgc tgtactgcca gtttgatggt gcatcgcgac ccggttgttc
     1741 cggagcgtgc aggtataagc tcaccgccac gcgcaggtag tagtaaaggc cgattgccga
     1801 accaacaacc acggcaccca ccagccacca caagtgtgcc tggacaccga ccgccagcac
     1861 gtagaactta ccgataaagc ccagcgtcat tgggatacca gccagtgaaa gcatcatcac
     1921 cgtcatcact gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga
     1981 atcagcatcc gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag
     2041 gctgctgaac agataaccga ccaggtaaac ccctaccgct tccatcgaca tctcgccggt
     2101 ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag
     2161 cagacgtttg atattggtct ggctcagcgc catcaggtta ccgaagatga tagaggcaaa
     2221 ggcgataatc gccagcacta cgcgaatcgc ttcactgtca cccaccggtg cgtaaaggaa
     2281 caaacgcatc accacgccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac
     2341 cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag
     2401 tttgaaaccg aggccaacaa tcatcaggcc aaaacctgcc agcaacagcg gctcgttgag
     2461 cataccgtcg ccgaggtttt tacccagtgc gacaaacgac aggtcgccag actgcgcata
     2521 caccagcgcc ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt
     2581 gatactggct tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg
     2641 caaagagatc agttcgatac cgaggaacag agacgccaga tggttggcat tcgccagcag
     2701 gatcccgccc agcgcggcaa ttaacaccaa caggtagaac tcgtccttgt tgtcgttata
     2761 gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc
     2821 ggtgtaaagc atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc
     2881 ctggccgaca aaccagagcg ataccagcgc cgcgttaagc ccaataaccg agagcgtagc
     2941 gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc
     3001 gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta
     3061 cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg
     3121 cgaggtatcc agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa
     3181 caggatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct
     3241 tttcgcttta ccgaagtaag cgcgatgcag catcgccagc gaataaacag aagcaaagac
     3301 cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat
     3361 cataaattcg ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa
     3421 cagcgacagt gctggcagcc atttcatttt gctccacaga ccgcccatca tgcgcatgtc
     3481 gcgggtatgg atacgttcat aaagctgacc acaaagaata aacagacccg ccgccgacaa
     3541 gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat
     3601 agcaatcagc acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc
     3661 ggtctgggcg aaggccatcc acgcaccata gaagatgccg ataacaccca gccacatagc
     3721 gattggcgcg aactctgccg acgcgttcgg gaacagcggt agggagaaac gcagcagacc
     3781 gtaagcggcg gttttcagca agatccccgc gaggtcaacg gaaccggcgg tcggtgcctg
     3841 ggagtgcgca tccggtaacc agccatgcag cggaaccacc ggcattttca ctgcgaaggc
     3901 gatgaagaag cccagcatca acaggtattc cacgccgtta gacatcggcg tattcagcag
     3961 ctcttcatag ttgaaggtcc agacgccggt cgcattgtag tgaacgaaaa ccagcgccag
     4021 gatggcgatc aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccgc
     4081 cgtgatacgc gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat
     4141 cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac
     4201 gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc
     4261 gatctctttc cacgaacaga gtaccgccag cacaccgagc agaccggtca gcacgaccat
     4321 cagcaacgac agaccgtcaa tggcgagatg aatagagata ccaaaacgcg ggatccacgg
     4381 catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc
     4441 ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggttacca gcgcgatcca
     4501 gcgcggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc cgataaaggg
     4561 aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag
     4621 gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg
     4681 gatgcgccgc gaagacggct tatccgacct acgttcttat atgcccaggg gcaatcccac
     4741 aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca
     4801 tggatgccac ataccagcgc agataaccgt tctcgcttaa cagcagacct ttacctgcaa
     4861 agcgggaaag gacagccggg atgttcatca ttgagttcag cggatcgcgt ttcagcaacc
     4921 aggcaatacc caggaacggc ttgacgaaca ctttgtcgta cagccagtca aagccccagg
     4981 cgttgtacca ccaggtaccc agcagacggc ccggcgcact gttggcgatg gaggtcacca
     5041 gagtacgttt acccagccac agccaggcag ccagcagaat gccgaccacc gcgaccacgc
     5101 cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca
     5161 cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa
     5221 tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt
     5281 ggaagacgat gaaaatcata cggaaggtgt aaagcgaggt cataaacgca ccgaccagac
     5341 ctgccaccat cagattgata tgtccattcg ccatcgcacc cgcgaggatc tcatccttac
     5401 tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc
     5461 agagataaac cagcggaata gatttacgca gaccacccat cttaaagatg ttctgttcgt
     5521 gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt
     5581 gggtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt
     5641 agccaatctg gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg
     5701 ccgcaaaacc ggccagcagc agcgtaaccg ccccaacaat gcccaccaga tgcagaactt
     5761 ccggcgtcat caggaacaga ccgtgggtac gggcgatcag gtagacaccc gcggttacca
     5821 tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcggcaagcc
     5881 atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg
     5941 cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc
     6001 ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag cgcgaaagcg aggaagacgt
     6061 cacccacacg ggtcacgacg aacgctttca ttgccgctgc gccattcttc ggatcggtgt
     6121 aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca
     6181 gcagcaggtt gtcggcaagc accagaacta ccatgctggc gatgaacagg ttggtgtaag
     6241 cgaagaagcg agagtagccc tcttcaccgc gcatatacca ggaggcgtac atgtgaataa
     6301 ggaaacccac accagtgacc accgagagca tggtcagcga caggccgtcc agcaccaggt
     6361 taaaaccgat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct
     6421 gctcaccgtt agcgaagaag tcaacgccga taaaggcagt taccagcgcc gccaggccca
     6481 cagagccaac gccgacgatc gccgagacgt tttcagacca gcgcccacgg gagaacgcca
     6541 gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat
     6601 ctcacttact gaatcaatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc
     6661 aaggccgata ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc
     6721 gtcggtctgg ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat
     6781 gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc
     6841 aagaacgaat aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc
     6901 tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct
     6961 tcacgaccaa cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccaattcc
     7021 accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta
     7081 ccgtcgatac cctgatcgtt aacaccgaga atggcgtaaa caatcaccac cagcatgatg
     7141 gctgacaaaa ttgccggacc aatccacacc tgcggtttca gccactggcg ttcctgttcg
     7201 atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca
     7261 cccgcataga cgataatttc cagcgcaccg gcgaagtaag cgcccagtga gaagaacacc
     7321 ccagagatcg ccagcagcga aataatcagg tacagcagcg cgtgtaccgg attggtatgg
     7381 gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc
     7441 attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg
     7501 cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg
     7561 ggtatttgcc cggaccggag atcagcagat cctctttctc gtaaaccaga tcctggcgct
     7621 tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt
     7681 cgcacagacc acagaaaatg cagcgtgaga agttgatgcg gaaaaattcc ggataccagc
     7741 gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcgc
     7801 agaggttaca ggctacgcaa cgctcttcgc cgtccggatc gcgggtcaga acgatacgac
     7861 cacgataacg gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg
     7921 cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtaccgaaa cctactaaca
     7981 attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc
     8041 cgttaccagc aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac
     8101 ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa
     8161 cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa
     8221 gaacagcgtc accatcagtg cagagatggt cacaatcccg atgtattcgc ccacgaagaa
     8281 cagaccgaac ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc
     8341 ttccggctgg tcaaacgggt gacggtgaca caccgccacg cccgcgatgg caaaggtaat
     8401 aaaaccaaag aattgcggga tgacgttcca cacatgcgcc tggctgttga cgatgtcggt
     8461 catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc
     8521 gtagctcagg gtctgcgcag aagcacgcat cgcccccagc agcgagtatt tgttgttact
     8581 tgaccagccc gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa
     8641 aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa ttgcaaaggc
     8701 cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacac gatccgagaa
     8761 tttcgggatc cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga
     8821 accgccccag ccaacacggt taggtccata acggttctgg aacagaccca gcaggcgacg
     8881 ttcgccaaag ctcatgaatg ccccacaggt gactaccacc agcaggatca ccaccgcttt
     8941 aaggacggtc agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc
     9001 ttgagatcct caagatgcgc gccagccagc accggagcaa tgccggacat acccatcggc
     9061 aagcccacct gccctgctgt cagcccttcg gcgatttcaa ccggcagcgt gaccgtgttg
     9121 ccatcgtaac taaaggagac gcgtgtacct gcgttcacac ccaacttcgc ggcatccgct
     9181 gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac
     9241 aattcatcgc tgccaaacag gtggtagtac ggcgcgatac gccatttccc gtcctgcggc
     9301 tggaagcgtg ccggtacgct ggtgaagtaa tccagaccgt tttcgctggt ttcaaacaga
     9361 cgcacgcccg gatcgccaaa acgcagtttg ccgcccactt cgtcctggaa tttgttccac
     9421 gcctgcgggg agttccagcc tggcgcccag gcaaacggca cttgcgaacg gtgcgcggtc
     9481 ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc
     9541 ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctatagcg atgcggttca
     9601 cgggccagtt tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgatacct
     9661 gccagttccg ggattttcgc cacaacagcg tcaatcacgt ggtcgagctg cgtccagtcc
     9721 acttcacggc tcagcagggt gctatgcagc gagtgcagcc agcgccagct ttccagcatg
     9781 acagtttggc tgtcgtaata agccggatcg taaacttgga agaaacgttg ggcgcggcct
     9841 tcgttgttga tcaccgtacc gtcgctttca gcaaagctgg cggcggaaag taccagatga
     9901 gcgttttcca taatcgctgt gcgttgatgg tcaaccacca tcaccagcgg cgctttagcc
     9961 agcgcagcat tcacgcgggt agcggaagcg tgacgatgca gatcgttttc cagcaccacc
    10021 accgcgtcgg cgcgtccggt ttccagttca gttaacgctt cttcaagcga tccaccgcct
    10081 ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc gacgtcagcg
    10141 ccgcgacctt tcagggcttt cgccacgtta gccgcagcct gaatcacttc agcgctacca
    10201 gcgttcgtcc cggagataat caacggtttc ttcgcaccag ccagtgcctg cacgatgacg
    10261 tcgattttgc tttgcagctc aggctctatg ccgtcaactg ccggtgcgga gttgtccagc
    10321 gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac
    10381 gccgcgatat catccagacg ggtgtcatca acgttggtaa caaacagcgg atgcttcgca
    10441 cgttgaccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgccgcc
    10501 atttcgcgcg ctttaccttt taccgcctgg cgcaccgcca gcgcaacgcg cgcgccggtc
    10561 tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc acgcagagcc
    10621 ggagtataaa tgccgccttc acgcagtact ttcagcgcca gttgcagacg ttcctgctca
    10681 ccgtgagcga taccggtata gaagttttct tcgcccacca gctcacgcag cgcaaagttg
    10741 ctttccacgc tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata
    10801 tctgctgcac cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc
    10861 tgtactggct gacgcggacg atctttcagg ttgacgtaac cgtaaccgaa acgaccacgg
    10921 tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg
    10981 ccataacgct cgccggggct gatgttacag ccgatggaac attgctggca gatgctcggc
    11041 gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtgaatacg
    11101 ccggtcgggc aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc
    11161 gggcgaccga agtagacgtt gtcgtgcgca ccgtaaacgc ccaggtccgt accgtcagcg
    11221 taatctttgt agtaacgcac acagcggtag caggcgatgc agcggttcat ttcgtgagag
    11281 atgaatggcc ccaaatcctg attacggtgg gtacgtttag tgaaacggta gcgacggaag
    11341 ctgtgtccgg tcatcacagt catatcctga agatggcagt taccgccctc ttcacatacc
    11401 ggacagtcgt gcgggtggtt ggtcatcaac cactcgacca cactttcacg aaactgtttc
    11461 gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc
    11521 accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa
    11581 gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac
    11641 agacaagctt ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga
    11701 attgtagcca ttagcatgct tccagttttc tcagtcagag actgagcgtt aatcgaaatt
    11761 cggttaccag cgctctttca gcaggtttgg ctgaatccca ttaatcaaat gggtattgct
    11821 gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa
    11881 cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca
    11941 cagttgctca agtgtttcga tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag
    12001 aattttcacg ctccacggca gaccgtcgcg gcatggcgta caccagccgc aggactcacg
    12061 ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc
    12121 catcgccagc gccgtaccca gacggcttcc cgctttaccg atactttcga attccatcgg
    12181 caggtcaagg tgcgcttcgg tcaggaagtc agtccccgct ccgcctggct gccaggcttt
    12241 gaatttcaga ccgtcacgca taccaccggc gtagtcttcg aggatctcgc gtgcggtggt
    12301 gccgaacggc aattcccaca gacccggatt tttcacccga ccagagaagc ccatcagctt
    12361 ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat
    12421 cgccggaacg ttacacaggg tttcgacgtt gttaacgcag gttggtttac cccatgcacc
    12481 ggaggttgcc gggaagggcg gtttcgagcg tgggttagcg cgacgccctt ccagggagtt
    12541 gattaacgct gtttcttcac cgcagatgta gcgccctgcc ccggtatgga cgaacagttc
    12601 gaaatcgaaa cctgttccca taatgttttt gccaagcaga cccgcttcgg tggcttcggc
    12661 aatggcacga cgcagattca ctgccgcttc gatatattcg ccgcgcagga agatatagcc
    12721 acggtaagct ttcagcgcaa acgcggagat gagcatgcct tccaccagca ggtgcggcag
    12781 ttgctccatc agcagacggt ctttataggt gcccggctcc atttcatcgg cgttacacag
    12841 taggtaacgg atattcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga
    12901 gaagcctgcg ccaccgcgcc ctttcagacc agagtctttt acctgattaa cgatttcgtc
    12961 cggagacaac ccggtcagcg ccttacgcgc gccttcgtaa ccgtttttgc tgcgatattc
    13021 gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg
    13081 agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc
    13141 agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg
    13201 cagcaagttg gcagcagcgt gaagcgaccg tcaaatgtcg tttgccctgg tttgatgttc
    13261 agttttttct ccagcgccgc ctgaataccc tggtaaccgt tgatgtgaca gaccacgctg
    13321 tcgcaataac ggatcacatg gcggcctact ggctgacgga agatctggct ataaaacgtt
    13381 gccacgcctt cgacgtcgct cgccggaata cccagcacat cggcgatcgc gtggatcgcg
    13441 ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca
    13501 cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc
    13561 tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca
    13621 taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg
    13681 ccgccggaat ttgctgcaaa tgcgcataac tcggcgtacg gatacgggtg cgataactca
    13741 tggtgctgcc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaaccatct
    13801 ggaaggattc attggcaggc atcaccggac cccacgacac ttgcaggaag tgggtgatca
    13861 gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg
    13921 ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatacgc agactctggc
    13981 gcaactcttc tactttcagc attacgcggg tgtagcagtc agaaacacca ccgcccaccg
    14041 gaatttcgaa gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt
    14101 cgatcccggt ggcacgcagg cccgcgccgg tggtgcccca ctccagcgcc tctttcgcgc
    14161 cgtaagcggc aacgccctgg gaacgacctt tcagaatggt gttttgcagc gccgctttct
    14221 cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcagg cgatcccagc
    14281 cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacgaaaac
    14341 cagtgattgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacactg
    14401 gcgtcatcgc gccgacgtcc tgaataaagg tggagatata cagcaggtga ctgttgatgc
    14461 ggaacagttc ggagagcatt acgcgaatga cgttaacgcg atccggcacg gtgatcccag
    14521 ccagtttctc taccgccagc acataaggca tttcgttaac gcagccgccg aggtattcga
    14581 tacggtcggt atacggaatg tagctgtgcc aggactggcg ttcgcccatt ttctctgcgc
    14641 cacggtggtg gtaaccgatg tctggtacgc agtcgacaat ctcttcgcca tcaagctgca
    14701 aaacgatacg gaaagcaccg tgcgcagacg ggtggttcgg accgaggttg aggaacatga
    14761 agtcctcatt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt
    14821 ccatctccag atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg
    14881 ccgggtaatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca
    14941 ggttcgggtg accgtcgaat gtaatgccaa atagatccca ggtttcacgc tcataccagt
    15001 tagcgttcgg gaataatttg gtaaaggtcg gtacgtgcag gtcgttttct gccagcgcca
    15061 ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat
    15121 ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa
    15181 acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gcccacttcc agtaattgtt
    15241 cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg
    15301 gcccaaaacg gttgcgcagt tcgccaatca ccggatcatc aagatgatcg cgggtctgcc
    15361 aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt
    15421 gactgtcggc gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaattccctt
    15481 tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggtaacg gcaatgcgtt
    15541 caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa
    15601 ccacccagga gagcggacga cgttctttac cgatagattc ctgcaacagc atcagtgcct
    15661 gcatgtacgc ttcaggacgc ggcggacagc ccgggatata cacatcaacc gggatgaatt
    15721 tatcaacgcc ctgcacaacg gaataaatat cgtacatacc gccggagttg gcacaggcac
    15781 ccattgagat aacccatttt ggttccagca tctggtcata caaacgctga ataaccggtg
    15841 ccattttggt aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac
    15901 gcaatacttc agcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct
    15961 caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt
    16021 taaccatgtc attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt
    16081 cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc
    16141 gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt
    16201 tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa
    16261 tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa
    16321 agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa
    16381 cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg
    16441 cggagccgac cgagtcgata ccggattcaa acggaacgtt ttttgacctc gcgcgtgcgc
    16501 gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa
    16561 gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg
    16621 cttactcatc aaaagtagcg cctgattctc tgctctttac ggcagatgga cgccacatcg
    16681 attcatgggg aggaataaaa aaaccttaca ataactgcag aaactatttt atacagctaa
    16741 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa ggtttcttca
    16801 cactgattta cacaatgaca acaacatttg catttggcgc cctttctccc cagatcattg
    16861 acgactccag gaaaattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    16921 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    16981 gatctgtgac acgcttttgt cattctataa caaaaatgca gcaacaaatt cacgtatttt
    17041 ttaacattat tgtagcagga gatttttttc aggtgattat ttgtgcgtac ggggcgtgaa
    17101 tctctggtga ttgaaaaatg aacagttttg tacgttctgc acgatacgag gaaggctttt
    17161 tactaaaaaa aagccgctgg ggtttaaaat aaacccagcg gttcgttttt acactattgt
    17221 ctcaggaatt atctatcgtc cgtcgattac tcgatatccc tttcaatcaa caatgagtca
    17281 tctccttccg gagcagacat tgggctgtat tgccacggat tatggtagct ctccatcgcc
    17341 tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag caaatattcg
    17401 gtatcaggta gcggcggtaa gccatctact ccactcagca cacgcaggtc cggactcatc
    17461 atctcgaccg gccttgccgt cacgccaaga ccggctttca ctgccgcacg aacggccgga
    17521 agcgtcgatg cgacataagc cagtcgccag ggaatatctg ctttattaag cgtcgccagc
    17581 accatatcgc gaaacggact tggatcatcc agcaacacaa gagggatcgg ctcacctttt
    17641 tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc
    17701 ttaaacgttg aaggacgatg ggtggttacc atcaaatcca cttcctggga ttccagcatt
    17761 tctgccatat aggcattacg tttaacgcgg acatccagcg ctagtttcgg ataaaccgaa
    17821 ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga agcaccgata
    17881 gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc attaaaacgc
    17941 agaatttttc tggcgtaacc aagaagttga atgccatgtt cagttaacag tttgttgcga
    18001 ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat ttgctgactt
    18061 acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt cagatcggca
    18121 acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg atttgcactt
    18181 atcatatatt atcacttact ggcggctcat actgagctgg ttaatgctgt gcacacacaa
    18241 acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc atccggaacc cggacgaaag
    18301 taaaaatgca tatgagttgc actaaaaaag tgactcacat tgttccgtta taatgcctga
    18361 agtagatcac agaatatatc ttca
//
LOCUS       DAZFRG010000088        18380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFRG010000088 DAZFRG010000000
VERSION     DAZFRG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.088"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..954
                     /locus_tag="ACHKUC_002332"
     CDS             175..954
                     /locus_tag="ACHKUC_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO6618582.1"
                     /translation="MEHKPTIRVLEILEALAAENQGVSLTHLSEKTGILKGTIFPILK
                     TLVEQRYISYDANTQLYNLGISCSILSRAFLDRSYWLKMVYKEMNNIVKECNEVCQMG
                     ILDGADVLYINKVQAAQTVQLVSHIGTRLPAIYSALGKAIICEYSDQQIRQLYPDGFV
                     PMTSYSVTTLEQLRQQLEDARVNGYAFDNREINEETVCYAVALKQRNKTLAAISISIP
                     VFRATKEKIADVTRILLNARSRIEQELNTLQDINIGIDSHH"
     gene            complement(1031..1762)
                     /gene="rlmB"
                     /locus_tag="ACHKUC_002333"
     CDS             complement(1031..1762)
                     /gene="rlmB"
                     /locus_tag="ACHKUC_002333"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO6618583.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1942..4383)
                     /gene="rnr"
                     /locus_tag="ACHKUC_002334"
     CDS             complement(1942..4383)
                     /gene="rnr"
                     /locus_tag="ACHKUC_002334"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO6618584.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(4422..4847)
                     /gene="nsrR"
                     /locus_tag="ACHKUC_002335"
     CDS             complement(4422..4847)
                     /gene="nsrR"
                     /locus_tag="ACHKUC_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO6618585.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(5052..6350)
                     /gene="purA"
                     /locus_tag="ACHKUC_002336"
     CDS             complement(5052..6350)
                     /gene="purA"
                     /locus_tag="ACHKUC_002336"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO6618586.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(6454..6651)
                     /gene="yjeT"
                     /locus_tag="ACHKUC_002337"
     CDS             complement(6454..6651)
                     /gene="yjeT"
                     /locus_tag="ACHKUC_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO6618587.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6733..7737)
                     /gene="hflC"
                     /locus_tag="ACHKUC_002338"
     CDS             complement(6733..7737)
                     /gene="hflC"
                     /locus_tag="ACHKUC_002338"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO6618588.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7740..8999)
                     /gene="hflK"
                     /locus_tag="ACHKUC_002339"
     CDS             complement(7740..8999)
                     /gene="hflK"
                     /locus_tag="ACHKUC_002339"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO6618589.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(9085..10365)
                     /gene="hflX"
                     /locus_tag="ACHKUC_002340"
     CDS             complement(9085..10365)
                     /gene="hflX"
                     /locus_tag="ACHKUC_002340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO6618590.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(10441..10749)
                     /gene="hfq"
                     /locus_tag="ACHKUC_002341"
     CDS             complement(10441..10749)
                     /gene="hfq"
                     /locus_tag="ACHKUC_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO6618591.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10835..11785)
                     /gene="miaA"
                     /locus_tag="ACHKUC_002342"
     CDS             complement(10835..11785)
                     /gene="miaA"
                     /locus_tag="ACHKUC_002342"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO6618592.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11778..13625)
                     /gene="mutL"
                     /locus_tag="ACHKUC_002343"
     CDS             complement(11778..13625)
                     /gene="mutL"
                     /locus_tag="ACHKUC_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO6618593.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTISLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAASWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(13635..14966)
                     /gene="amiB"
                     /locus_tag="ACHKUC_002344"
     CDS             complement(13635..14966)
                     /gene="amiB"
                     /locus_tag="ACHKUC_002344"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO6618594.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPVVAKRVETPAVVAPRASEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14985..15446)
                     /gene="tsaE"
                     /locus_tag="ACHKUC_002345"
     CDS             complement(14985..15446)
                     /gene="tsaE"
                     /locus_tag="ACHKUC_002345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO6618595.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYSLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSSGELLLARLAG"
     gene            complement(15418..16965)
                     /gene="nnr"
                     /locus_tag="ACHKUC_002346"
     CDS             complement(15418..16965)
                     /gene="nnr"
                     /locus_tag="ACHKUC_002346"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO6618596.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTHSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16964..18103
                     /gene="queG"
                     /locus_tag="ACHKUC_002347"
     CDS             16964..18103
                     /gene="queG"
                     /locus_tag="ACHKUC_002347"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO6618597.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEQFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(18086..18139)
                     /locus_tag="ACHKUC_002348"
     CDS             complement(18086..18139)
                     /locus_tag="ACHKUC_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618598.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         4260 a         5170 c         4568 g         4382 t
ORIGIN      
        1 gagacctgca taaaaaacaa tcgcagttct ttttttatcc gttttatgac cagtatcacg
       61 ctaaaaaagg actgatgggc tcaacagcag tcaaggaacg cgagggggtt aatttacttg
      121 aattataatt ctccctagaa tggtgcatta ctcatggtaa ggaaaatagt cattatggaa
      181 cataagccta ccatccgggt gttagagata ctggaagcac tggcagcgga gaatcagggt
      241 gtttcattaa ctcacctttc ggaaaaaaca ggcatcctga aaggaacgat ttttcccatc
      301 ctcaaaacgc tggttgaaca gcggtacatc agttatgacg ctaataccca gctttataat
      361 ctggggatct cctgctcgat tctgtcccgt gctttcctcg acagatcgta ctggttaaaa
      421 atggtttaca aagagatgaa caacattgtc aaagagtgca atgaagtttg tcagatgggg
      481 atcctggacg gtgcagatgt tttatatatc aataaggtgc aggcggcaca aaccgtacaa
      541 ctggtttcac atattgggac tcgtctgcct gctatctatt ctgcgctggg aaaagccatt
      601 atctgtgaat acagtgatca acaaattcgg caactctacc ctgacggttt tgttcccatg
      661 acatcgtata gcgttacgac cctggaacaa ttgcgccaac aacttgagga tgccagagta
      721 aatggctatg catttgataa cagggaaatc aatgaggaga cggtatgtta tgctgttgcc
      781 ttaaaacagc gaaataaaac gcttgccgcc atcagcattt caatacccgt atttcgtgcg
      841 acaaaagaaa aaattgccga tgtcacgcgt atcctgctga atgcacgttc gcgtattgaa
      901 caggaactca acaccttaca ggatatcaat atcggtattg attcccacca ttaagggtat
      961 tttccattcg aagcccgata cgtaaaaatt atcgggctaa caaaatatcc attgaatgga
     1021 tagcgatgaa ctagctgcgc tggcgcaccg cttcaaataa gcaaattccg gtcgcaaccg
     1081 aaacgttcag ggaagaaacg cttccagcca tcgggatgct gatcaactca tcgcaatgtt
     1141 cacgagtcag gcgacgcata ccttcacctt ccgcacccat caccagcgcc aggcgtccgg
     1201 tcattttgct ctgatagagt gtatgatccg cctcgcctgc cgtaccgacg atccagatat
     1261 tctcttcctg caacatacgc atggtgcgcg caaggttagt cacccgaatc agcggaacgc
     1321 tttctgccgc gccgcaggct acttttttcg ccgtagcgtt gagctgtgcg gagcgatctt
     1381 tcggcacaat caccgcgtga acgccagcag cgtccgcgct acgcaggcac gcgccgaggt
     1441 tgtgcggatc ggttacaccg tcgaggatca gcaggaacgg ttgatcgagc gaagcgatca
     1501 aatccggcag atcgttttcc tgatactgac gtcctggctt cacacgggcg ataatgccct
     1561 gatgcacggc accgtcgctt ttctcatcga gatactgacg gtttgccaac tggataacca
     1621 cgccctggga ctcaagggcg tgaatcagcg gtaacagacg tttatcttca cggcctttta
     1681 aaataaagac ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc actgcgtgga
     1741 tgccgtaaat catttcgctc attaatgtac tcgttgttta cgttttgcct gatgcactac
     1801 gtttatccgg cctacatgat ctctgcaata tattgaattt gcgtgattat gtaggccgga
     1861 taaggcgttc acgccgcatc cggcatgaac aaagcgcatt ttgtcagcaa tctaactctc
     1921 ttcttttaaa gagggtattg atcactctgc cacttttttc ttcgccgcac gcttcgcttt
     1981 ggtcgctgcg gctattttct gcgttttcgc cgatggcttt ttcgcttttc tcgcgtcttt
     2041 cttcgccgct ttcggcttcg tttttttctc accgcggaag gcgctgtctg gctcaaagtt
     2101 taccttttta ccgacctgac gacgcttgcc gccttttttg cctgcatcgc cttttttcgc
     2161 tttctcgcgc gccgttttac cgacattgcg cggtgcgcgt tcgctggaga tcaggctaaa
     2221 gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga acttccacgc gatcgcccag
     2281 gcgataagtc tggccgctgg attcacccat cagccgttgc cctacctggt caaagcgata
     2341 gtagtcattg tccagcgaag agacatggac cagaccatca atgaacaagt cgtccagacg
     2401 gacgaagaag ccaaagccag tgacgctgga aattacgcct ttaaagacgt tacctacctg
     2461 gtcgagcatg aagtcacact tcagccagtc agcgacatcg cgcgttgctt cgtcggcacg
     2521 acgttccgcc atcgaacagt gctgacccaa ttgcagcatc tcttccatcg aataatggta
     2581 gccgccggtt tcagtggtgt tgccctgatg cccctgctct ttcgccagca gatatttaat
     2641 ggcgcggtgc agcgtcaggt ctggataacg acgaatcggc gaagtaaagt gcgcatagga
     2701 ctgcaatgcc aggccaaagt gaccacggtt ttctggatcg taaatcgcct gtttcatcga
     2761 gcgcagcagc atggtttgca gcatttctgc atcaggacga tcggcaaccg actccagcag
     2821 ctccgcgtag tcacgcggtt ccggcttatt accgcccggc agttccagcc ccagctccgc
     2881 cagcactgaa cggaaagagg taatcgcttc ggtgctcggc ttgtcgtgaa tacggaacag
     2941 tgccggttct ttggctttct caacaaaacg cgccgccgag atattcgcca gaatcatgca
     3001 ctcttcaatc aacttgtgtg cgtcgttacg ctgagtctgt tcgatacgtt caatacggcg
     3061 ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat gaaatcccgc cgcgttcttc
     3121 acgggcttta tccagcactt tatagaggtt atgcaactct tcgagatgct taaccagtgg
     3181 ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata tgccagactt tggtgtaggt
     3241 cagacgcgcg tgagagctca tcaccgcttc gtagaatttg tagccagtca ggcggccttt
     3301 cgacgaaacc gtcatctcgc acaccataca caggcggtct acctgcgggt tgagcgaaca
     3361 caggccgtta gagagcactt ccggcagcat cgggataacc tgcgaaggga agtacaccga
     3421 cgtgccacgg ttacgcgctt ctctgtccag cggcgttgac ggacgcacat agtagctgac
     3481 gtcggcaatc gcgacccata aacgccagcc gccgccgcgt tttttctcgc agtaaactgc
     3541 atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc agcggtaaat cgcgcaaatc
     3601 gacacggccc gcttttgctt cttccggcac ttcttctttc agcccggcaa cctgttgctc
     3661 aacagcctgc ggccagatat acggaatttc atgggtacgc agagcgatat caaccgccat
     3721 gccggtgccc atattatcgc caaggacttc gacgatttta cccaccgctt tggtgcggcg
     3781 agtcggacgc tgagtcagtt cgactacgac cacaaagccc atccgcgcgc ccatgatctg
     3841 atcgggcggg attaagatat cgaagctcag acggctatcg tcaggaacca caaagccgac
     3901 gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt tttggcacca gtacgcggac
     3961 aatacgcgct tcacgacgac ctttacggtc agcgcccagc ggctgagcca gcacctgatc
     4021 gccatgaatg caggttttca tctgctcgct ggagagatac aaatcatctt tacgcccttc
     4081 aacccgcaga aagccgtagc catcacggtg gccaataacg gtacctttca ccaggtcgag
     4141 gcgttccggc agcgcatagc actgacgacg agtgaagacc agttgaccat cgcgctccat
     4201 cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg ccttcaatgt gcagttctac
     4261 cgccagctca tcacggctgg ccggtttttc acgtttggtt aaatgttcga ggataaattc
     4321 ccgactaggg atgggattcg cgtatttttc agcttcgcgt tcctggaaag gatcttgtga
     4381 catctcggtt cctccgttgt catctctgat gaagattttc gtcactccac cagcaataat
     4441 ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg tgtagttatc cagttccgta
     4501 agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc gacaggcagg tgtaatgtgg
     4561 caaaactcac tgctgcaatt caccagcgat aagggctcca gctcgcgcac cacatcacca
     4621 atacgtatcg cactcgccga tttacccagg cgaatgccgc cattttttcc acgaacagca
     4681 gtcacgtagc cggcacgact aagttgattg attattttga ccatatgatt acgggagacg
     4741 ccgtagacgt cagtcacttc agaaatactg gtcatccgcc cttctggcaa tgacgccatg
     4801 tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta actgcacatc aacctcaaaa
     4861 gggaaatcgg gaaaaaatac atttatattg atgataaacc agccacaagc tgtgtcgcta
     4921 atttatttca gttcggggaa ggaaaaaagc gaggatttaa cactgtgtcg gatagcgtaa
     4981 tggcttaacc gacacagcaa tgtatcacac tgttagcccg gcaggcaaaa tatctgccag
     5041 gcgtaccaga attacgcgtc gaacggatcg cgcagaatca tggtttcagt acgatccgga
     5101 ccggtagaga tgatatcgat cggcacacca gtcagctctt caatacgctt gatgtagttc
     5161 agcgccgcct gcggcagacc gctacgatct ttcacgccga aggtggattc agaccagccc
     5221 ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt cagctgccag cggagtggtc
     5281 gtcacttcac gaccatccgg catacggtaa gccacacaga gtttcacctc tttcaggcca
     5341 tccagaacgt ccagtttagt caggcagaag ccagacaggg agttcagctg taccgcacga
     5401 cgaacggcaa cggtgtccag ccagccggta cgacgacgac gccccgtagt tgcgccgaat
     5461 tcgttaccct gcttgcagag gaactcgcca gtttcatcaa acagttcggt cgggaacgga
     5521 cctgcaccta cacgagttga gtaagctttg aggataccca gaacataatc aacataacgc
     5581 gggcccaggc cggaaccggt cgccacgcca ccagcagtgg tgttggaaga agttacgtac
     5641 ggataagtac cgtggtcgat atccagcagc gtaccctgcg caccttcaaa catgacgaaa
     5701 tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt caaccaccat agaagtcagg
     5761 atgtcggcaa cagccatcgt atcatccaga actttctggt aatcaaccgc ttcagctttg
     5821 tagtagttaa ccaactggaa gttgtgatat tccatcactt ctttcagttt ttcagcgaag
     5881 gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac gtgctacttt atcttcataa
     5941 gcaggcccga taccacgacc ggtggtgccg atcgctttcg cgccacgcgc tttctcacgc
     6001 gcgttatcca gcgcaacgtg ataatcaagg atcagcggac atgcttcaga cagcagcaga
     6061 cgctcacgaa cggggatgcc acggtcttcc agttctttca tctctttcat cagcgcggcc
     6121 ggagacagca caacaccgtt accgatgatg ctggttacat tctcgcggag aatacctgat
     6181 ggaataagat ggagaacggt tttttcaccg ttgattacga gagtatggcc tgcgttgtga
     6241 ccgccctggt agcgtacaac atatttagcc cgttcagtca gaagatcgac gatcttacct
     6301 ttaccttcgt caccccattg ggtgcccagt acgacgacgt tgttacccat ttttcaaaat
     6361 caccgtttgc ttaaaaatgg attctaccat cgctttttca gagttacagc actttttgca
     6421 ctcaaaaatg acctcgatca gtctgttttt tgttcagcca atcgttttcc tcaacatgta
     6481 gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa cgacgtaaaa tattatcggg
     6541 caaattggtc atcgcagaga tcatcttctt ccatgccttc gggtaaagca tcggccctaa
     6601 accttccagt accaaaacca gggcaagcgc cagccagatt gtcgaattca ttcattttcc
     6661 ttatagaaaa agaaaaccac cgacatccct gaggatgcgg tggctttatt gacctgtacc
     6721 gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc atgtagcgga agaaatcgct
     6781 atccgggctc atgaccatca cgtcctgatt gccagagaag ctgttctcat aagcacgcag
     6841 gctacggatg aatgcgtaga agtccggatc tttactgaat gcatcagcaa acagtttggc
     6901 tgcttcggca tcgccttcac cacgcatgat acggccctga cgctcagctt ctgccagcgt
     6961 tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc gcttcttcct gaccttgtga
     7021 acggtgacga cgcgctaccg cttcacgctc ggcgcgcata cggttgtaga tcgcttcaga
     7081 cacttcggta ggcaggttga tctgcttaat tcgcacatcg acaacttcaa tacccagcgc
     7141 cgccatactg ttcgggttga tgaccggaac tttgcccttc gtctcagccg ttacgcgctc
     7201 tgccgcttcg gcaatggcgt tatctgccgc cggggtagta acttcatctt ctgtacccgc
     7261 agaaccggag ttcagcgcgt cacgtacttc gagggtcaga cgaccacggg aatcggtgac
     7321 gatgtctttc acgtccaggc gaccaatttc agaacgcaga cggtcagaga acttacgttt
     7381 caacagcact tccgcttgcg aaatgtcgcc accacccgtt gccaggtagt aacggctgaa
     7441 atcgctgatg cgccatttga tgtaagagtc gacgatcagg tctttcttct ctttggtcac
     7501 aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg tcgagcattt tcaccgtttc
     7561 aatgaacggt atcttgaaat gcagacccgg ctcataaacc agaggtttgt tgtcatcgtc
     7621 acgcagtacc ttaccaaaac gcagcgtaat accgcgctca ccttctttga cgacaaagac
     7681 agacatgtaa agcactacca gcacgatgat gataatcgcg ataactgact tacgcatcgt
     7741 tattccccct gacgctggta gtcgttacgc tgcgcgttgg cgcggcgttg gtccataata
     7801 tcgccctgac tggtggacga cgtgttgctt gctccactgg ttgtggagga agaggctggc
     7861 ggcagacgca gcagattgct ggcaccgtta tcgctcttcg ccgcaggggc gttaccacct
     7921 ttcagcatct ggtctaacgg cagaaccatc aggttgccac ctttatcgtt aaccagcact
     7981 ttgcgggtgt tgcccaacac tttttccatc gtctcgatat acagacgctc gcgagtaatt
     8041 tccggcgcgg ctttatattc cggcagaagt ttagcaaagc gcgccacttc accctgggct
     8101 tccaggatgg tctgggcctt gtacgcacgc gcctcttcga ggatacgttg cgcctgaccg
     8161 ttcgcacgcg gctgaacttc gttggtatac gcttctgctt cacgaatgta ttgctgttcg
     8221 ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta cttcttccgg cggacgagca
     8281 gcctggaagt tgacgtccag cagagtgatg cccatgtcat acggacgaat cgtctcttcc
     8341 agttcgcgct gagtatcgct acgaatcacg gtacgacctt ccgtcagaat gcggtccatg
     8401 gtgtatttac cgataacccc gcgcagggcg ctgtcggtag cctgacgcag actgtcatcc
     8461 gggctggtca cgctatacag atatttttcc ggatcggtga cgcggtactg cacgttcatc
     8521 tcaacgcgca ctacgttctc gtcagacgtc agcatcacac cagaagctgc cagttcacgc
     8581 acggcttcca cgttcaccgg tttgacttcg tcaataaacg tcggtttcca gttcagaccc
     8641 ggctcaacca gatggctgaa tttaccaaag cgtgttacca cgccgcgttc ggcttcttta
     8701 atggtataga aaccactggc cgcccagata atgacaatcg ctgccgctgc gatggtaacg
     8761 acgcgaccgc caagctgcgg gcgcgggcct tgcgatgaac tgccaccgcc agatccggtg
     8821 cctttaccgc cgcccagacc accgagcttt ttgctcagtt tgcggaagat atcatctaaa
     8881 tcaggtggcc cttgatcgcg accgcctttg tttccatttc cctcagagtt gccgccaggt
     8941 ttgctgcttc cccacgggtc gcggtcttgt ccgttattac cgggctgatt ccacgccatg
     9001 tttgtgctcc atatttgtta tgcggtgatc cctgttgtct ttcgacgcca gaggatatga
     9061 ctccacgctt cagacgctac gccgttagat caggtaatcg atcaacgccg gttcttgttt
     9121 acagaggcga cgccagtcaa cgatcggcat acgaacttgc agacttacgc tgccgtcctc
     9181 ctccatccac tctttttcta ttgcctgaag ctgataaaaa cgacttctca ggcgcccttc
     9241 ctgcggcggc agacgcaatg tatgctgcgc cacctcgcca gaaagacgct ccgtcaaagc
     9301 ctgaaaaagc tgtggtatcc ccgctccggt ctgcgcagaa agccagacac ggatcggttt
     9361 gttctcttca tctctgtcaa tacgcggttc gaaatcgtcc agcatatcga tcttgttcat
     9421 caccagcagg gttgggatct cgtgagcatc aatctcttca agaaccgtat tcaccgcttc
     9481 gatgttttct tgtacacgca catccgccgc gtcaatgacg tgcagcagca atgtggcttg
     9541 ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga tcatgcggca ggtggcgaat
     9601 aaaccctacg gtatctgcca acacggtttc accgacatcc gcaacgtcaa tacgccgcaa
     9661 cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag acccgcgctt cggtgattcg
     9721 attgaaaaga gtagatttac cggcgttggt atatcccacc agcgaaacag taggaacgtc
     9781 ggctttgata cgcgattgcc gcccctgctc acgctgcttt tcaactcttt ccaggcgcga
     9841 ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg gtttcgagct gggtttcacc
     9901 cggaccacgc aaaccaatcc cgcctttctg tctttcaagg tgggtccagc cacgcaccag
     9961 gcgcgtagcc agatggcgca actgcgccaa ctcaacctgc aacttaccct catgggtacg
    10021 cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg tcgatgacac gacactcgca
    10081 caaacgctcc aggttacgct cctgcgccgg gctcagggca tggtcaaaaa gaacgaccga
    10141 agctcccgtc gcttttacgg cttccgcaat ttcaactgct ttaccttcac ctacaaaata
    10201 ctttgggtgc ggcgctttac ggctaccggt aatcacctgc aatgcttcga caccggcgga
    10261 agagaccaga gattcaaact cctggaggtc ttccatatct ttgtcttgcg taaaatagat
    10321 gtgtaccagt accgcctgct caccagcatc ataacggtca aacaagcgta taaccctcta
    10381 aatagatcag cggggaacgc aggatcgctg gctccccgtg taaaaaaaca gcccgaaacc
    10441 ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc tgcgcgctgc taccatgatg
    10501 atagttactg ctggtaccgc caccggcgtt gttactgtga tgagaaaccg ggcgagacgg
    10561 gacaacagta gaaatcgcgt gcttgtaaac catctggctg accgtgtttt tcaacaggat
    10621 cacgaactga tcaaaagact cgatttgtcc ttgcagctta ataccattca ccaaataaat
    10681 agaaactgga acacgttccc gacgcagtgc gttcaggaac ggatcttgta aagattgccc
    10741 cttagccatt ctctcttttc cttatatgct tatttgtact ttgaaccttt cgattctgaa
    10801 aaaattgcgc acgatacgtc tcaattgtac acattcagcc tgcgatagca ccaacaacct
    10861 gtaatacttc gtcacgcgcc tgttctggtt tttcactgtc aagccagtga accccttccc
    10921 aaccacgcag ccaggttatc tgccgcttcg ccaactgtct cgtggcgcaa acacctcgat
    10981 aaaccatttc atcgtatgag atttcgcctt caaggtaaga ccacatctgg cgataaccca
    11041 cgcaacgaat ggaaggcaaa tccgtatgca aatctcctcg ggcaaaaagc gcccggactt
    11101 ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg ctcaattcgt tgatggagca
    11161 gttcacggct cgccggggcg atggcgaact gatgcacctg atacggtaga gcgtctcctg
    11221 acgtttgcgt cagttccgtt aaagttttac ccgaaatgaa aaaaacttcc agtgcccggg
    11281 aaagcctttg tggatcattt ggatgaatcc ttgctgccgc aaccggatcg atctcctgaa
    11341 gttgacgatg caatgactcc caaccttgct ctgccgcctg ttgctcaatt ctggcccgta
    11401 cttccgggtc tgccgacggt agcggcgaca acccttccag caatgccttg aaatacaaca
    11461 tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt gatatcggcc atttccgcca
    11521 gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga cggatcgcga atattcagca
    11581 atcggtgcgg cgcggcgagt aactcttcag cgttcggctt cgccgtcccg atatccatcc
    11641 ctttgtaaat aagggcagaa tcaacgctta tcaactctac tggtaaaatt ttacgcagct
    11701 caatggctaa cgccgtttta ccggaggccg tcggccccat caaaaaaatc gccttaggca
    11761 ggctcgcctt actgatatca ctcatctttc agggctttta tcgccggatg taaatcaaca
    11821 gattgtaaca gaccacccgg cggcgttttc acaagttgcg gacataaccg ttccacgtcc
    11881 gccagcaggg ttatggcctg tgccattgac cactgcgcat gttcgctcat cagatttcgt
    11941 gcaatccact gcgcaatatt gccaggttcg aatacggact gcttcgccag gtagcctatc
    12001 agttcaggaa tcaagatttg taaattttgt tggcgtaagg gtaaaggcac ggccctgatg
    12061 gtcacatgct gcgcatcgga ctggaaatca atacccagtt ccgccagggc agactgcgct
    12121 ttttctaatg ccgatttttc ttcgccagaa acttttagcc gcaacggaat aagcagcggt
    12181 tgggcgcaaa ctggcacttc acccggcgtc aattgtgcct gacgcagcca acgttctgcc
    12241 actggcaagg ataaaagtga aatgttgcca tcgcgctcaa gcaacgcaca gtcggaatgg
    12301 acgatagtca gtacccgacc aaaactctga ctgttcgccg caagtgcagg ttcctgcggt
    12361 tctggcgctt ttggtttttg catcggagcg ggcgtttgca aaagctggcg atacacttca
    12421 ccttgctgtt tctggtaacc aggctgcgca ttcggccagg aggcagccgg acgactgcct
    12481 gaggcgggcg caggagagta gcgcggagct accggctcac gaactgccgg ttcagcaaag
    12541 tgattgcgcc cagccgccac gcgattttcc ggaatggcac gcggtgcagg ttgtggttca
    12601 tcgtccagcg gtagcggcgt ttccagttgc tgttgtagca cgctcagcac gccctgatag
    12661 ataaagtcat gcaccagacg cgactgatgg aaacgcactt cgtgtttggc agggtgcacg
    12721 ttgacgtcca cctgatgcgg gtcgatctcc agatacaaca caaatgccgg ttgctgatcg
    12781 gcccccagtt tgtcttcgca ggcctggcgg atcgcgtgat tgatcaggcg atcgcgcatc
    12841 atgcgaccgt tcacgtagca atactgaatt tctgccagtg cgggcgtagt gtgatttgga
    12901 tcggccaccc agccgcgcag cgtgagatcg ccgtgttgcc attcaatcgc cagcgcttgt
    12961 tcgagaaaag cggtgccgca aatcgcgcct aaacgccgtt ctttttgccc gccttccggc
    13021 actgcacgat actggcgtac gattttgccg ttatgcgaca gactgatagt gacgtcgaaa
    13081 cgcgccagcg caatgcggcg gatgatttca tcgatatggt taaattcggt tttctcggtg
    13141 cgcaggaatt tgcgccgcgc cggggtgttg tagaacaggt ccagcacctc cagcgtcgtt
    13201 cccaccggat gcgccgccgg tttaaccgtc acgtccatat cgcgcccttc ggcataggcc
    13261 tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg tcaggcggga aaccgaactg
    13321 atactcgcca gcgcctcacc gcgaaagccc agactgataa tggcttcgag atcgtccaga
    13381 gaggcgattt tactggtggc atgacgcgcc agcgccagcg ccagctcgtc ttttttgata
    13441 ccgcagccgt tatcacgaat gcgaataagt ttcgccccac cgcgttcgat atcaatatcg
    13501 atacgcgtcg cacctgcatc gaggctgttt tccaccagtt ctttgactac cgacgcaggt
    13561 cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt gtggcggtaa gacctgaatt
    13621 ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg tcgtcaccgt actggccgtt
    13681 tgtgccgttg caccctgcgg cgcagattgc atcggatgcg caaggaagta attgcgcagg
    13741 cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt cgctcgccag caaacgttct
    13801 tcgctgttgt tactgataaa accggtttcg accagtactg acggaatatc cggcgaacgc
    13861 agaacgccga gactcgcgtg ttctggtcgg cgtttatgta tttcgccaat gcgttgcaac
    13921 tgactgatca tactggtcgc tacatcatac cctacccgct gggaatgacc gaactgtaaa
    13981 tccagcaccg cctggcttaa ataggggtca gactgactgt tcgccagcac atcacccgcc
    14041 ccgcccagca gctccgactg tttctcgtgc tgctccagcc agctggccat ttcgctgttg
    14101 gcgcgacggt tagagagcac ccatacggaa gcgccagtcg cactgcggtt cggtgctgca
    14161 tcggcatgaa tcgacacaag gaaattggcg ttttgcttac gtgccacatc actgcgcccc
    14221 atcaccgaga taaagtaatc cccgtcacgg gttaaaacgc ccttaaacat cggatcgtta
    14281 ttgagcaaag tacgcaattt acgcgcgatg gcgatggtga catttttctc ccgcgtacca
    14341 ccggggccga tagcgccagg gtcctgaccg ccgtgtccgg catcaatagc gatgataatt
    14401 ttatcgccag tgttagccgt cgcgcgggct gccggacgcg ttaccgtatt actgctgata
    14461 acacccgtag tgcggttact ttccgtttta aacggattgc gcgccggttc gctggcgcgc
    14521 ggtgcgacaa ccgcaggcgt ttcaacgcgt ttcgcaacca ctggcggagg cggtggcggc
    14581 gcatcggcgt taatcgtaaa gacaacagtg tagttgctgc cattctgccg cttcaccgct
    14641 tcggttttac cgttttcggt gagatcgacc accagccgta gcgtttgtgc atctttaggc
    14701 gttccggagc gaatcgcctt caccagatta ttaccgctga acaacaacgg cagcccctga
    14761 attacccccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    14821 taatcaggat cgccaataaa actcaacgtt atccgtgcct gttgattacc gttagaaacc
    14881 tgaatatcag agagcgtcgc ggcacccacc ggcgcgcaca gcagcagcaa cgtcgctacc
    14941 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    15001 gccagcaaca attcacccga agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    15061 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    15121 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    15181 tcctcgggat cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgaa
    15241 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc tagagcctgt
    15301 aaaaagcccc ggctaaaggt ggttttacct gcacctaaat cgccatacag atagattacg
    15361 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    15421 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    15481 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    15541 tacgtcagct gccgcaccgt gcgcgacaca gcccgcacag gctgcatcat aagggctcat
    15601 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    15661 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    15721 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt gggcgcaatg
    15781 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg
    15841 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    15901 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcaggg cttttttccc
    15961 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    16021 ggtaagagag tccatagtca gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg
    16081 cgcaatgttc tcactgtggg tcagtactcg gactaaacca gcaccagcac gcagcgccgc
    16141 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccaataa tcaccagccg
    16201 cccgtgatcg cctttatgcg aagtcgggcg acgcggtatc agccagtgag aaagttgttc
    16261 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    16321 gtcaaaatgc agttgtccgg taacatcccg tgcttttcca gtgagcaagc ctggtttcag
    16381 cgcaataaaa gtgatggtgt gatcggcgtt gattaccgcg cctggcgtag cgccggtttc
    16441 agccagaagg ccggaaggga tatcaaccgc cacaatcggc gcagggtgag tattagcgtg
    16501 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtgc cgagcagcgc
    16561 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgcctcc
    16621 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcactttc
    16681 ctgggccagc agcgtgacat caataccgat cgctttggcc agccgcgcga ccacgtagcc
    16741 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggataagc
    16801 cgaacgacac acctggaatg cggcttcgcc agcgcgaagc atcagctcat agagtgtgag
    16861 ccccagcgca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagactgt
    16921 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    16981 tctcaatcaa ttagcgcaaa atattaaaca gtgggggctg gaactgggct ttcagcaggt
    17041 aggtattacc gataccgatc tcagcgcgtc cgagcccaaa ctgcaagcat ggctggacaa
    17101 acaataccac ggcgaaatgg actggatggc gcgtcacggt atgctgcgcg ctcgccctca
    17161 cgaactattg cccggtacgc tgcgtgtgat cagcgtgcgg atgaattacc ttcctgctaa
    17221 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    17281 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    17341 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttcttgagcg
    17401 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    17461 tgaggccggc tcgttcttct ttttaggcga attgctggtc gatattccgc tacccgtgga
    17521 tcaaccagtc gaggaaggat gcggtaaatg cgtggcctgt atgacgattt gcccgaccgg
    17581 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    17641 actggaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    17701 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    17761 tttcagcccg cgtaagccgc tacacgctcc ggaactcatt gagttattcg cctggagcga
    17821 agagcagttt ttaaaagtca cggaaggttc ggcgattcgc cgtataggtc acctgcgttg
    17881 gctgcgtaat atcgccgtag cattaggcaa tgccccctgg gatgaaacga ttttgacagc
    17941 gctggaaagt cgtaaaggtg agcacccact tcttgatgag cacatagcgt gggcgattgc
    18001 gcagcaaatc gagagacgaa atgcgtgcgt ggtcgaagtg caattaccga aaaaacagcg
    18061 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    18121 aataaaaata aaaacgcatt gcaattcaag ccggaaaaaa tcgtcaagtg atcgatacga
    18181 caaaacgaaa tcttaatttt taacttatct atcaataggt taaaaaaata ctattcaccg
    18241 tgcgaagttt ctttgaaatt aagattacgg cctgaggctg tggataactc tgtttacaag
    18301 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt
    18361 gagagaagaa tttggagcgg
//
LOCUS       DAZFRG010000089        18232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFRG010000089 DAZFRG010000000
VERSION     DAZFRG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.089"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            62..1282
                     /locus_tag="ACHKUC_002349"
     CDS             62..1282
                     /locus_tag="ACHKUC_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6618599.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMFQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1255..1884
                     /locus_tag="ACHKUC_002350"
     CDS             1255..1884
                     /locus_tag="ACHKUC_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO6618600.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(1885..3045)
                     /gene="ybdL"
                     /locus_tag="ACHKUC_002351"
     CDS             complement(1885..3045)
                     /gene="ybdL"
                     /locus_tag="ACHKUC_002351"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO6618601.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3154..4242
                     /gene="hcxA"
                     /locus_tag="ACHKUC_002352"
     CDS             3154..4242
                     /gene="hcxA"
                     /locus_tag="ACHKUC_002352"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO6618602.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNHQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4252..4449)
                     /locus_tag="ACHKUC_002353"
     CDS             complement(4252..4449)
                     /locus_tag="ACHKUC_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6618603.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4562..6667)
                     /gene="cstA"
                     /locus_tag="ACHKUC_002354"
     CDS             complement(4562..6667)
                     /gene="cstA"
                     /locus_tag="ACHKUC_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO6618604.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6848..7261)
                     /gene="entH"
                     /locus_tag="ACHKUC_002355"
     CDS             complement(6848..7261)
                     /gene="entH"
                     /locus_tag="ACHKUC_002355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO6618605.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7264..8010)
                     /gene="entA"
                     /locus_tag="ACHKUC_002356"
     CDS             complement(7264..8010)
                     /gene="entA"
                     /locus_tag="ACHKUC_002356"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO6618606.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8010..8867)
                     /gene="entB"
                     /locus_tag="ACHKUC_002357"
     CDS             complement(8010..8867)
                     /gene="entB"
                     /locus_tag="ACHKUC_002357"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO6618607.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTINAW
                     WKLLSREVK"
     gene            complement(8881..10491)
                     /gene="entE"
                     /locus_tag="ACHKUC_002358"
     CDS             complement(8881..10491)
                     /gene="entE"
                     /locus_tag="ACHKUC_002358"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO6618608.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKLGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEAGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10501..11676)
                     /gene="entC"
                     /locus_tag="ACHKUC_002359"
     CDS             complement(10501..11676)
                     /gene="entC"
                     /locus_tag="ACHKUC_002359"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO6618609.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11865..12821
                     /gene="fepB"
                     /locus_tag="ACHKUC_002360"
     CDS             11865..12821
                     /gene="fepB"
                     /locus_tag="ACHKUC_002360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6618610.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(12825..14075)
                     /gene="entS"
                     /locus_tag="ACHKUC_002361"
     CDS             complement(12825..14075)
                     /gene="entS"
                     /locus_tag="ACHKUC_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO6618611.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITMLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14186..15190
                     /gene="fepD"
                     /locus_tag="ACHKUC_002362"
     CDS             14186..15190
                     /gene="fepD"
                     /locus_tag="ACHKUC_002362"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO6618612.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15187..16179
                     /gene="fepG"
                     /locus_tag="ACHKUC_002363"
     CDS             15187..16179
                     /gene="fepG"
                     /locus_tag="ACHKUC_002363"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO6618613.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDTPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16176..16991
                     /gene="fepC"
                     /locus_tag="ACHKUC_002364"
     CDS             16176..16991
                     /gene="fepC"
                     /locus_tag="ACHKUC_002364"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6618614.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(16988..18121)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUC_002365"
     CDS             complement(16988..18121)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUC_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO6618615.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLSLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLRPSLPVKKD
                     GPGKSIIVILSALIGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         4324 a         5163 c         4673 g         4072 t
ORIGIN      
        1 atcctataat tagcggattc atctcacgaa tcaccccgta acgttcagag agcgttttta
       61 gatgtctatt tataaaatcc cgcttccact gaatattctg gaagctgcaa aagaacgtat
      121 tacctggaca cttaataccc taccccgtat ctgtgtttct ttttcgggtg gtaaagattc
      181 cggattaatg ctgcatctga cagccgaaat tgcccgacaa atgggcaaaa aaatctgcgt
      241 tttgtttatc gactgggaag cccagttctc atgcactatt aactatgttc agtcattacg
      301 tgagttttac gccgatgtca tcgaagagtt ttactgggtt gcgctcccgc ttacgacgca
      361 aaattccctt tcacaatacc aacccgaatg gcagtgctgg gaacctgatg tcgaatgggt
      421 gcgtcagccc ccgcaagatg cgataaccga tcctgacttt ttctcctttt accagcctgg
      481 tatgaccttc gaacaatttg tgcgtgagtt tgccgaatgg ttttcgcaaa agcgtccggc
      541 ggcgatgatg atcggcatcc gcgcagatga gtcctacaac cgttttgtcg ccatcgccag
      601 tttaaataaa caacgttttg ccgatgataa gccctggacc accgccgcac caggcggtca
      661 tagctggtac atttacccca tctacgactg gaaagtggct gatatctgga cctggtatgc
      721 taaccatcaa cagctctgta acccactgta taatataatg tttcaggcag gcgtccctct
      781 gcgtcatatg cgaatttgcg aaccttttgg cccggagcaa cggcaaggat tatggctcta
      841 tcacgttatc gaaccggatc gctgggctgc tatgtgcgca cgagtcagcg gggtaaaaag
      901 tggcggcatt tacgccggac atgataatca tttttatggt catcggaaaa tcctcaagcc
      961 agaacattta gactggcaag aatatgcatt attgctgctc aatagcatgc cggaaaaaac
     1021 ggctgagcat taccgcaata aaatcgctat ttatttgcac tggtatcaga aaaaaggcat
     1081 cgaagttcca caaacccagc aaggggacat tggcgcgaaa gatatcccct cctggcggcg
     1141 aatatgcaaa gttttactca ataacgatta ctggtgccgg gcattatcat ttagtcctac
     1201 gaaagcgaag aactatcagc gttataacga acggataaaa ggaaaacgtc aggaatgggg
     1261 gatactatgc aacaacgatt aacgcaggat ttaacccaat ttctcgccag cttgccggaa
     1321 gatgatcgca tcaaagcgat taatgaaatc cgcatggcga tccatcaggt tagccccttt
     1381 cgtgaagagc cagtggattg cgttctgtgg gttaaaaaca gccaacttat gcctaatgat
     1441 tacaacccaa acaacgtggc accacccgaa aaaaaactgc tgaaaaaatc cattgaaata
     1501 gatggtttta cccaacccat tgtagtcacg catacggata aaaatgctct ggaaatcgtg
     1561 gatggttttc accgccatga aattggtaaa ggctcatcat cgttaaaatt acgcttaaaa
     1621 ggttatttgc cggtgacctg tctggaaggt acgcgtaatc aacgcatcgc cgcgactatt
     1681 cgtcataatc gcgctcgtgg acgtcaccaa atcactgcca tgtcagaaat cgtccgagaa
     1741 ctcagccagt tgggatggga cgataataaa attggcaaag agctgggaat ggacagcgac
     1801 gaagtgttgc gcctgaagca aattaacggc ctgcaagagt tgtttgccga ccgtcaattt
     1861 tcccgcgcct ggacggttaa ataactacag ctggcgcagg cgttctgctg ctgccagcaa
     1921 cgtcgattcc ttcttggcaa aacagagacg aatcagttta tgtgggaagg gatcggcgca
     1981 aaacaccgac agcggaatcg ccgctacgcc gtgctcccgc gtcagccact ggcaaaactc
     2041 aacatcatcc agcgtagata cggcgctgta atccaccagc aaaaagtatg taccttcgca
     2101 cggtaaaatc tccagccgac tttcatttaa ggcattcacc agaatatcgc gcttctggcg
     2161 ataaaagtcc ggtaacgcaa gataatgctc aggttctgca cgtagcatat cggcaagcgc
     2221 caactgtgcc ggggtattca ccgaaaaggt cagatactga tgcactttgc ggatctcggc
     2281 gctgatgggc gcaggcgcaa cgcaataacc cactttccag ccggtcatat gataggtctt
     2341 gccaaacgat gacaccgcca ccgctcgctc acgcaattgc ggatgcgcca gcacgctggc
     2401 atggccctgt tgtgaaaaat tgatgtgctc gtagacttca tcgctaatga caaaaatctc
     2461 gtgcccggcg atcgcctgcc acagagcggc gaaatcagtc tgctgccaga cagttgcact
     2521 ggggttatgc ggggtgttga ggatcaccag tcgggtgcgc tcgcttaaca atgcggcaaa
     2581 ttcctgccag tcaacgcgaa aatgcggcgg ttgcagtgca atacgtttca ctattccccc
     2641 cgaaagtgcg atggcggggg cgtaactgtc atagctggga tcaaaacaaa tcacttcgtc
     2701 gccattgcgc accaatgcgg ttatcgccgc gtataacgcc tccgtcgccc ctgccgtcac
     2761 ggtgatattg ctgtcgacat ccggttgata gccatacaga cgttccgttt tctgagcaat
     2821 cgcctcgcgc aaggcctgca cgccggtcat gggtgcgtat tggtttgccc cctgggcaac
     2881 atggtacgcc agccgctcct gtaaatagcg cggaccatca aaatcaggaa agccttgcga
     2941 aaggttaatg gcctggtgtt gctgcgccag cgcgctcatc cgggtgaaaa tagtggtgcc
     3001 aagttgtgga agtttgcttt gtggaatcag agggttattt gtcattttta ttgtacctga
     3061 cgaagttgcg tgttgaaggc actataacac gatgctagta tttggcaatc aagacgttta
     3121 gatgtctaaa taaaacaata aggacaacac aacatgcctc acaatcctat ccgcgtggtc
     3181 gtcggcccgg ctaactactt ttcacatccc ggaagtttca atcacctgca cgattttttc
     3241 actgatgaac aactttctcg cgcggtgtgg atctacggcg aacgcgccat tgctgcggca
     3301 caaaccaaac tgccgcctgc gtttgaactg ccaggggtaa agcatatttt gtttcgtggt
     3361 cattgcagcg aaagcgatgt gcaacaactg gcggcggagt ccggtgacga tcgcagcgtg
     3421 gtgattggcg tcggcggcgg cgcactactc gacaccgcga aagccctcgc ccgccgtctc
     3481 ggtctgccgt ttgttgccgt tccgacgatc gccgctacct gcgccgcctg gacaccgctc
     3541 tccgtctggt ataacgatgc cggacaagcg ctgcattatg agattttcga cgacgccaat
     3601 tttatggtgc tggtggaacc ggagattatc ctcaacgcgc cgcaacaata tctgctggcg
     3661 gggatcggtg acacgctggc gaaatggtat gaagcggtgg tgctggcccc gcaaccagaa
     3721 acgttgccgc taaccgtgcg gctagggatc aataatgcgc aggctattcg cgacgtcttg
     3781 ttaaacagta gcgaacaggc gctgagcgat cagcaaaatc accagttaac gcaatcattt
     3841 tgcgatgtgg tggatgccat tattgctggt ggtgggatgg ttggtggtct gggcgatcgt
     3901 tttacgcgtg tggcggcggc ccatgctgtg cataacggtc tgaccgtttt gccgcaaacc
     3961 gagaagtttc tccacggcac caaagtcgcc tacggaattc tggtgcaaag cgccttgctg
     4021 ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc agcgttttca tctgccgact
     4081 acgctggcgg agctggaagt ggatatcaat aatcaggcgg agatcgacaa agtgattgcc
     4141 cacaccttgc gtccggtgga atccattcat tacctgccgg tcacgctgac accagatacg
     4201 ttgcgtgcag cgttcgaaaa agtggaatcg tttaaagcct gacgtacata tttagcagca
     4261 gcgcgcgccg ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa actcctcgta
     4321 ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt tcgacatagt tgtcgtaatc
     4381 aggcatacca atcatcagtt tcgccgcctg acctaaatat tttccggctt ttgccagtga
     4441 atcaaacatc taaacctccg gtaatacaaa tgccctcctg aattaagagg gcttaattgc
     4501 tatcaacacg ctcgggatga gagtaagggg agcgctcagc acattccccc tcactctgac
     4561 tttagtgcgc gcctttcgcc tgcgccacga tctcctcgac attttccggc atcggctcat
     4621 atggcgtttc tttcgccgtt ggcttcggct ctttcaatgc cgccagtgcc gtcttaatcg
     4681 agaacagcgc cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac
     4741 ggttgttgaa cacgagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc
     4801 tgtcgatcat tgcctggaac ttgttagcaa tcgccaggaa tccgactttc gcatccgggc
     4861 taaacgcttt ctgccagcct gcggtcaagg tacaaatcag cagccaggcc gttggtacca
     4921 gcgccaccca ggcgtaacgt tgacgtttca ttttgaacag caccacagca caaagcatca
     4981 gcgccatccc tgccagcatc tggttggcaa taccaaacag cggccacaag gtgttaatac
     5041 cgcccagcgg atcgaccacg ccctgatgca ggaagtagcc ccacgccagc acgcacagcg
     5101 ccgttgccag caggttagca ggcagtgaat cggtccgttt caggcccgga gacaccacgc
     5161 ccagcagatc ctgcaacata aagcgcgcag cacgcgtacc cgcatccacc gccgtcagaa
     5221 taaacagtgc ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcatgccgc
     5281 ccagcgcacc gtgcagaatg taggccatcc ccaccgccag ggtcggcgca ccgcccgcac
     5341 gagaaatgat cgactgttca cccacttcgc tggcaatctg gtttagcgta tccggggtaa
     5401 tcgcaaagcc ccagctactc accacctgcg cggcagaagc aaccacatcc gccgtccctg
     5461 ccggagccag caccgccatc gggctgttca tggcgaagta cacacccgga tcgatgatac
     5521 aggcggaaac cagcgccata atcgccacga aggattccat caacatccca ccgtagccga
     5581 taaagcacgc ctgcccttcg ttcgccagca tcttcggtgt agtgccggaa gagatcagtg
     5641 catggaagcc agacaccgcg ccacaggcaa tggtgataaa caggaacggg aacaggttac
     5701 cggtccatac cgggccagtg ccatcgacaa atttagttag cgcaggcatg gtcagcgtcg
     5761 ggcgcataat caaaatgccc accgccagac cgacgatagt cccgattttc aggaaggttg
     5821 agaggtagtc acgcggagcc agcagcaacc acaccggcag caccgccgcc acaaaaccgt
     5881 aacccaccag catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccacgtcg
     5941 ggctttctgc cacccagccg ccagagataa tggcgaaaat aaggaacacc agaccaatca
     6001 ccgacacttc gccaatacgc cccggacgca aatagcgcag gtagatcccc ataaacagcg
     6061 ccagcggaat ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttaa
     6121 ccacgatcat cgccagcacg gcaaggataa tgaccatgat cataaagcag gccaccagcg
     6181 ctatcacccc ggcggttggc cccatctctt ctttgaccag ctcacccagc gagcgaccgt
     6241 cacggcgcgt ggaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc
     6301 ctgcgagcag ccagatcatc cctggcaggt aacccatttg cgccgccagt accggcccaa
     6361 ccaacggacc tgctccggca atggccgcaa aatggtgacc gaacagcact tttttgtccg
     6421 tcggcacata gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa
     6481 ccgccagcac atttttggcg atatacagcc cataaaaacg gtaagcgatc agatagatac
     6541 agaccgacgc caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt
     6601 atcccagagc aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt
     6661 tgttcatagt tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca
     6721 tctttgtaca atttaacaac acagcaacca tgtaaatcgg agttgaaaca aatatttaac
     6781 ttaacaatgt taactcgatc actccgttaa ccgaattttc ctgcaatctc attttattct
     6841 gtcgggttca tcccaaaacc gctgtgccca gccgacaggt gcagcaacgc cgcccctgtt
     6901 catcgaaaac gacgatttcc cagctctgat tttgtcgtcc aagatgcagc ggttggcaga
     6961 caccgcgcac ctttccctca gacaccgggc gatgatgtgt ggcgttaagt tccgtgccca
     7021 ccacacactg tccgtcgcgg gtcatcataa atccggccat cgatcccagc gtttccgcca
     7081 gcgccgccga cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg
     7141 gcatttcggc ttccagcaca tcatcgccca gacgggtata cacaattccc agatgcgcca
     7201 ccattgtgtt atcgctggtg gcattcagct cgtcgagcgt taaatggcgt ttccagatca
     7261 tgcttatgcc cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct
     7321 ggcgagatca gaggcgagga acaaaatcgt attggcaatc tcctgcggac gggcgatttt
     7381 ccctagcgga atgccgagtt taaactgctc gccaaagccg cgaatacgct gctcttcggc
     7441 gtcatcgcta acccacagcg tgcgttgcat atcggtgtcg gtggagcccg gcgaaaccac
     7501 attacagcgc acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag
     7561 cgccgctttc gacgcgccat aagcactcat accaatgcgc ggcgtgtgtg cggcgtcgga
     7621 cgcaacagta acaatcgccc cgccccgctg acggcgaaac tggttcatgg tctgctgaaa
     7681 caggttaaac gcaccgccga cgttgaccgc aaaagtctgc tgccagtcct ctttactgag
     7741 ctgatcggtc gcgcccatgc gcaaaattcc cgccgcattg accagcacgt ccagtcgctc
     7801 cgtttcagct aacagtcgct gacatacctg cgccacctgc gcagcatcgg caacatccat
     7861 tacttcggtc gcaaaaggat attgctcctg agtaaacgct tgatcaaaac ctgtaacttt
     7921 cgcccccgcc tcaacaaatg ccagcgccgt ggcatagccg atacctttcc ccgcgccagt
     7981 tacccagaca tttttaccgc tgaaatccat tatttcacct cgcgggagag tagcttccac
     8041 caggcgttga tggtcgggtt tttcgccagc atgacaaagt cgatatcgcc atgcactttg
     8101 cgccagcgcg ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg
     8161 tcgtcatcga agggttcatc ggactcgtcc agcaacggca ggatcacctc acgcagcgcc
     8221 gctttgctgg cggggatagg tgctggcagc aattcttcgg tcatcaccac ccgaccagaa
     8281 cgcccggcca catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca
     8341 tccgccacca taaacggttt aatatcgcgc ataaatgcgt ccgttgcggt ggtcatacag
     8401 ccaatgtggg catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt
     8461 tgctccagcg gagaacgatg aaatgcgctg tagcgccatt tcaccagcac cgtgtcgtcg
     8521 gcatctggcg tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggccaggc
     8581 ccccacatat cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg
     8641 taataaaccg ggatattgtg ctgtttgcag tagtcgcgta gtgccgcaat attcgcgatc
     8701 acctgctcca tcatcgggca gttctcaccc cagaagctga caaaatagtc ctgcatatca
     8761 tggattaaca acgcggcacg ttgcggttca aaggcccagt cgactttatt ctgcggaata
     8821 tcatgagact ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct
     8881 tcaggctgat gtgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac
     8941 cgccgtcagc ggaagtgaat ctacacactc cacgcgatcc ggtaatttaa attcggcaat
     9001 accctgttca cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag
     9061 ataagcacag cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcgtagat
     9121 caccgccgga tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc
     9181 acggttgatc tgatctttct cgcgcccctg cacagtgatg taaccctctg gatcaataga
     9241 gatcagatcg ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg
     9301 gcttttgtaa tagccacgga aggtgtacgg cccgcgcgtc atcaggcgtc cagcttcccc
     9361 ttgcggcagt ggatttcctt cggcatcggc aacccatact tcatcgtccg ggcacattgg
     9421 gtaaccctgg gtatggataa ttttctccgc actatcatca agacgggtgt agttcaccag
     9481 cccttccgcc atgccaaaca cctgctgcaa ctgacagcca atctcagcgg gaatacgcgc
     9541 cgcaagggtg gcagaaagac gggcgccgcc gacctgtaac agtttcagcg aggcaagctg
     9601 cgcccggctt tcgccttcag ccagtgcctg caaccacagg ctgactgctg gcggcaccag
     9661 cgcggtgacg ttcacctgat gtttttcaat caatgggaag caaagcgtgg cgctggggtc
     9721 ggcagccaga acgacagtgc cgccagcgag aaataccccc aacgatcccg gtgaactcat
     9781 ggcgtagtta tgagccgccg ggatcgcgca caggtagcgc gtctgttgtg tgaactgaca
     9841 aatctcgacg ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt
     9901 cggagtgccg gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggagatgg
     9961 cgtggcagta aaatcctcag ccggatggtt aatcgcatcc tgcaagttat gctcaccgct
    10021 gtcgttgagc agctgcacca cgcgaatcga agaatgctct gcaacaaatg tgttgaggaa
    10081 atcatccccg ctaaatagcg catgttggcg atcggcaatc agtaacgccg gttcaatctg
    10141 gctggcatag gcgttcagtt cactacgctg atggctgaac agcgccagca ccggcgcaac
    10201 gcccagtttc agcagcgcga aaaaggtaat gtaaagttca gcgacgttac ccagttgtac
    10261 cagcgcggtt tcaccaagtt taatgccctg acggcgcaaa ctacaggcga ggttatccgc
    10321 cgcctgattc agctcccgat aactcaactg ccgctcgccg tcgataaccg cgatgctgtc
    10381 actcgcagcg tggcgagtca gaatgtcggt cagtggcaaa tcctgccagt agcctttttc
    10441 ccgatagcga cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc
    10501 ttaatgcaat ccaaaaacgt tcaacatggt agaaagtttg acgcccgttt cgcgccactc
    10561 acccaacggt gaggacgcag gcacaatccc cgctccggca aacagacgca cctgattttc
    10621 ccgcagcttc gcgcagcgga tggtcaccac ccattcaccg ttaccttcgc tgtcacacca
    10681 acccacaatg ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgagt
    10741 cgcggcctga tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aagccaaagt
    10801 cagtgcgttt tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt
    10861 cggcgtggta atcaattgtg gagaggaggg aacgtgtaac tcactactgc gtttgcgcag
    10921 tacctctttc atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag
    10981 cagacgatta cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc
    11041 taacggaatg gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc
    11101 cagcagaacg ccaccatcag ccaacggaac atggaagttg taactaaccg ggttttgcgc
    11161 aatcaaccgt tccagcaata cgccactatc aatggcggcg tcagtagtga tatcaatcaa
    11221 ccgtgacaac accactttgt cgacctgcgg cgtggcggta agtgcggcag cacgggcaac
    11281 catctgttca aacgtggttt gttccggaat ggcctgacgt tccaccacgt tcagcgactg
    11341 gctgcgggtg aaacggcgtg cggaagcttg tttttcctga cgggagaacg actgccagga
    11401 ttcgggaata tacagcgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac
    11461 cggatttttg atgccctgcg ctttggcatc ggcaaacaac gcggcgagtt tttgctggaa
    11521 gggactgtcg ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga
    11581 cgtcgtaaaa ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat
    11641 ggtctgctgt acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag
    11701 gctttaataa tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta
    11761 tgtcaactct tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat
    11821 gaaaatgaga agcattaact tcattaataa caggaagctg acttgtgaga ctcgccccgc
    11881 tctaccgcaa cgcccttcta ttaacaggac ttttgctttc aggaatagcc gcagttcagg
    11941 ccgccgactg gccgcgtcag attactgaca gccgtggcac tcataccctg gaaagccagc
    12001 cgcagcgtat agtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc
    12061 cggtgatcgc cagcggcgcg accacgccaa ataaccgcgt cgcggatgac cagggctttt
    12121 tacgccagtg gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac
    12181 cgagtgcaga agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaaccggcg
    12241 gcgattcggc gctggcgctg tacgatcagc tttctaccat cgccccaaca ttaatcatca
    12301 attacgacga caaaagctgg cagtcgctgt taacgcagct tggcgaaatt accgggcatg
    12361 agaaacaagc ggcagagcgg attgcacagt ttgataagca actggcggcg gcgaaagagc
    12421 aaatcaaatt accgccgcag ccggtcactg ccatcgtcta taccgccgct gcacacagtg
    12481 ccaatctctg gacgccggaa tcagcacaag ggcagatgct ggaacaactc ggctttacgc
    12541 tggcgaagtt gcccgcaggc ttaaacgcca gccaaagcca gggcaaacgc catgacatca
    12601 ttcagcttgg tggagaaaat ctggctgcag ggttaaatgg cgagtcacta ttcctgttcg
    12661 ccggtgatca gaaagacgcc gatgctattt atgctaatcc gctgctcgcg cacctgcctg
    12721 cagtacaaaa caagcaggtt tatgcgctgg gaaccgagac gttccgcctg gattactaca
    12781 gcgccatgca agtgctggat agacttaagg cgctgtttta agcattaact gtcggacgct
    12841 gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag cagcaacaac
    12901 acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg aagcaaccgg tgtcatcatc
    12961 gctcccaaac cgcccagcag cgccgcgcct atggcatcgc ccgtcacgtt ttgcgccgtc
    13021 cacaaaccgt taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtatat
    13081 tgcagcaacg aactaaccgc gctcagccag ccgaacagcg ccagacaaac cacgcctaaa
    13141 atccacatcg gcatcaggcc aaacagacca atggcgagga acgcccccaa tgtggagagc
    13201 aacatcaaca gacctggccg tacgctatgc gccagttttc cactggttaa cgcgccaata
    13261 gccgcaccga gcgggattgc cgcatagaga aagccaattt gcgccgccga catctgccag
    13321 ttgtcagcca gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaacccccc
    13381 agcaacgcaa tccctcccac cagcgggctg gcgagtagaa aacgaaatcc tgccagtaat
    13441 gatttcaacg gatgctcacg cggctgcggc ggtggtggca acgccggaag gcttaacaac
    13501 ggtagcatgg taataaatgt gcccgccgcc gccagcccgt agttccaggc tacgcctccg
    13561 gtcgccagca gtaaaccgcc aatcatgggc gaaatcaccg atcccagacg cacagtcaac
    13621 atagtgatcg ccccggcctg cattaagttt tcacgcccta ccagtgcagg tgtcgccgcc
    13681 agtagcgccg taacgccaag tgatgcgaaa aaaccatccc ataaaccaag taaatagatt
    13741 gccagcaatg acggctccgg cagcagcgcg ttcaggcaca gtccaatgaa gccaatgcca
    13801 caggtgccgc gcgccagcaa aatcactttt ttacgctcat agcgatccgc cagcacaccg
    13861 ccgaccatca ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc
    13921 caggtggaat gcgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga
    13981 gaaacaattg agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac
    14041 aggctgaggt taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt
    14101 taaataccta ttcttaggct gcacatgcta acatatccaa ataagatcga taacgataat
    14161 taatttcatt atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc
    14221 cgtgcccgga ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc
    14281 aaaatcactc cccgcttccg tagtgctgga ggccttctcc ggcacctgcc agagcgccga
    14341 ctgcaccatc gtgctcgacg cacgactgcc gcgcaccctt gccggtttac tggcaggcgg
    14401 tgcgcttggc cttgccgggg cgttaatgca aaccctcacc cgaaacccac ttgccgatcc
    14461 tggtttgctt ggcgtgaacg ccggtgccag ttttgccatt gtgctgggtg cggcgctgtt
    14521 tggttactct tccgcacagg aacaactggc gatggccttc gccggggcgc tggtggcctc
    14581 gttgattgtt gcctttaccg gcagtcaggg cggcgggcag ttaagtccgg tgcgtttaac
    14641 cctggcaggc gtggcgctgg cggcggtgct ggaagggctg accagcggca tcgccctgct
    14701 taatcccgac gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa
    14761 tctacatacc ttaaaagtgg tgctgatccc ggtgcttacc gctggagcaa ctgcgctatt
    14821 actgagccgc gcgctgaaca gtttaagcct tggcagtgac accgcgacgg cactgggcag
    14881 tcgcgtagcg cgcacacagt tgattggtct gctggcaatt accgtgcttt gtggtagtgc
    14941 gacggcggta gttggcccga ttgcctttat tggcctgatg atgccgcaca tggcgcgctg
    15001 gctggtaggt gccgatcatc gctggtcgct gcccgtcacg ctactcgcta cccctgccct
    15061 gctgctgttt gccgatatca ttgggcgggt gattgttccc ggcgaactgc gcgtttctgt
    15121 agtcagtgcc tttattggcg caccagtgct gatcttcctc gtacgacgta aaacgcgagg
    15181 tggcgcatga tttacgtctc tcgccgatta attatcacct gtctgctgtt gctaatagct
    15241 tgtgtgatgg caggcgtgtg gggattacgt agcggtgccg tcacgctgga aacttcgcag
    15301 gtattcgccg cgttgatggg cgacacgccg cgcagtatga cgatggtggt caccgaatgg
    15361 cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg
    15421 atttttcagt cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc
    15481 ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg gtcaggacct gacggctatc
    15541 gcgctggcag caatggtagg cggcattgtc acttcgctgt tggtctggct gctcgcctgg
    15601 cgtaacggta tcgacacctt tcggttgatt attatcggta tcggtgttcg cgccatgctg
    15661 gtggccttta atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcgggt
    15721 ttgtggaatg ccggatcgct caatggcctg acgtgggcaa aaacctcgcc ttccgcaccc
    15781 atcattatat taatgttaat tgccgccgcc ttgctggtgc ggcgaatgcg cttgctggaa
    15841 atgggcgatg ataccgcatg tgcgctgggc gtcagcgtcg aacgttcgcg actgttaatg
    15901 atgctggtcg ccgtagtgct taccgcagct gccactgccc tagcggggcc aatttccttt
    15961 attgctttag tcgcgccaca cattgcccgc cgcatcagtg gcaccgctcg ctgggggctg
    16021 acccaggcgg cgctgtgcgg ggcgttgtta ctgctggtgg ccgatctctg cgcccaacaa
    16081 ctgtttatgc cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac
    16141 cttatcgtct tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc
    16201 gcggcgaaca gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag
    16261 aaatacctga tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccacgt
    16321 tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg
    16381 agcacattca acattacgcc agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa
    16441 atgctaccac accgggcgat atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc
    16501 atcaaccgct gtttacccgc tggcggaaag aagatgaaga ggcagtcaca aaagcgatgc
    16561 aggccacggg aataactcat ctggcggatc aaagcgtgga caccctttct ggcggacaac
    16621 gccagcgagc gtggatcgct atggtgctgg cccaggaaac ggcaattatg ctgctcgatg
    16681 aacccacgac ctggctggat atcagtcatc agattgattt gctggaactg ttaagcgaac
    16741 tgaaccgcga gaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc
    16801 gttacgccag ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcaccga
    16861 aggagattgt cactgctgaa ctgatcgagc gcatttatgg tctgcgctgc atgattattg
    16921 acgatccggt tgccggaacg ccgctcgtgg tgccgctcgg acgaacggca ccttcaaccg
    16981 caaagattta aactaagtgg tctgccatca tggcatcctg ttttctggat gccatcgcat
    17041 ggcgcaacag cacaccacca caagccacca tcccgccgat caacgcggaa aggatcacaa
    17101 taatcgactt acccggaccg tcttttttta ctggcaatga cggtcttaac tgatatttaa
    17161 acggcgtaaa attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactggc
    17221 gattacgtaa ttcgccgttc agttccgcaa cgtcagtaac cgctttttca atttccagtt
    17281 tgcgttcaat accgtcagca ccgagagaaa tagagaaatc ggggtcatct ttaacggcct
    17341 gaccattact gtatacgggc tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat
    17401 tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt
    17461 tttctttttc aaactgggtt ttgatctcca gtttattacg gacgttttct atcgactctt
    17521 tcaccaccaa cgcagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac
    17581 tggttggcgc ggtaaaactt agcgtccagg aggtatacag tgacggttca tcttttttct
    17641 tactggcatt gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca
    17701 aatccagttc gtcgattttc gcctctttta attggtccat tacataaggt gatgagcgca
    17761 ggtactcttc tagtaagcta acagactgaa acttcttgat aaacaggtta aatgcttctg
    17821 tacgatcaat tttgatatcc agatccagca cacgaagctt agtgaatgtt ttctccagct
    17881 cttgccactg aacaggttct ggaggcgtga caaccgccgc gctagtccat ttttgcggca
    17941 ggatgaaaga gatcagcaag cctgcgcagg caaacgcaaa aacgaccgcc atgaccgttt
    18001 ttttggcccg ccataaaact tcaattagac taagtaaatc aatttcatta ttactgggcg
    18061 acgccagagg atactcggga aaatgagctt cacttccctg tttaatattc agtgatgaca
    18121 tggggtataa cctgaaaatg agtccaataa gagtaaatct ccacggcccc cactctacca
    18181 gttggtgaga tttatccgaa gctgaagttt gtaaattact cccactgaat gc
//
LOCUS       DAZFRG010000090        17970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFRG010000090 DAZFRG010000000
VERSION     DAZFRG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.090"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(132..878)
                     /gene="cutC"
                     /locus_tag="ACHKUC_002366"
     CDS             complement(132..878)
                     /gene="cutC"
                     /locus_tag="ACHKUC_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO6618616.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(892..1458)
                     /gene="yecM"
                     /locus_tag="ACHKUC_002367"
     CDS             complement(892..1458)
                     /gene="yecM"
                     /locus_tag="ACHKUC_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6618617.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1674..3407
                     /gene="argS"
                     /locus_tag="ACHKUC_002368"
     CDS             1674..3407
                     /gene="argS"
                     /locus_tag="ACHKUC_002368"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO6618618.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3460..3852)
                     /gene="flhe"
                     /locus_tag="ACHKUC_002369"
     CDS             complement(3460..3852)
                     /gene="flhe"
                     /locus_tag="ACHKUC_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO6618619.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(3852..5930)
                     /gene="flhA"
                     /locus_tag="ACHKUC_002370"
     CDS             complement(3852..5930)
                     /gene="flhA"
                     /locus_tag="ACHKUC_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6618620.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5923..7071)
                     /gene="flhB"
                     /locus_tag="ACHKUC_002371"
     CDS             complement(5923..7071)
                     /gene="flhB"
                     /locus_tag="ACHKUC_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO6618621.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7280..7924)
                     /gene="cheZ"
                     /locus_tag="ACHKUC_002372"
     CDS             complement(7280..7924)
                     /gene="cheZ"
                     /locus_tag="ACHKUC_002372"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO6618622.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7935..8324)
                     /gene="cheY"
                     /locus_tag="ACHKUC_002373"
     CDS             complement(7935..8324)
                     /gene="cheY"
                     /locus_tag="ACHKUC_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO6618623.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8339..9388)
                     /gene="cheB"
                     /locus_tag="ACHKUC_002374"
     CDS             complement(8339..9388)
                     /gene="cheB"
                     /locus_tag="ACHKUC_002374"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO6618624.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     VKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISVGQAIRI"
     gene            complement(9391..10251)
                     /gene="cheR"
                     /locus_tag="ACHKUC_002375"
     CDS             complement(9391..10251)
                     /gene="cheR"
                     /locus_tag="ACHKUC_002375"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO6618625.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQKILRRFVPLLKPDGLLFAGHSENFSHLERRFMLR
                     GQTVYALSKD"
     gene            complement(10366..10496)
                     /locus_tag="ACHKUC_002376"
                     /pseudo
     CDS             complement(10366..10496)
                     /locus_tag="ACHKUC_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(10542..12203)
                     /gene="tar"
                     /locus_tag="ACHKUC_002377"
     CDS             complement(10542..12203)
                     /gene="tar"
                     /locus_tag="ACHKUC_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO6618626.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12348..12851)
                     /gene="cheW"
                     /locus_tag="ACHKUC_002378"
     CDS             complement(12348..12851)
                     /gene="cheW"
                     /locus_tag="ACHKUC_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO6618627.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12872..14836)
                     /gene="cheA"
                     /locus_tag="ACHKUC_002379"
     CDS             complement(12872..14836)
                     /gene="cheA"
                     /locus_tag="ACHKUC_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO6618628.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     GQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGDERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14841..15767)
                     /gene="motB"
                     /locus_tag="ACHKUC_002380"
     CDS             complement(14841..15767)
                     /gene="motB"
                     /locus_tag="ACHKUC_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO6618629.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAINRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15764..16651)
                     /gene="motA"
                     /locus_tag="ACHKUC_002381"
     CDS             complement(15764..16651)
                     /gene="motA"
                     /locus_tag="ACHKUC_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6618630.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16778..17356)
                     /gene="flhC"
                     /locus_tag="ACHKUC_002382"
     CDS             complement(16778..17356)
                     /gene="flhC"
                     /locus_tag="ACHKUC_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO6618631.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17359..17709)
                     /gene="flhD"
                     /locus_tag="ACHKUC_002383"
     CDS             complement(17359..17709)
                     /gene="flhD"
                     /locus_tag="ACHKUC_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO6618632.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4301 a         5000 c         4417 g         4252 t
ORIGIN      
        1 cacccgctca acagatcacg gcaatcgttc gttttttata ctgctcaggg ataaaaaaag
       61 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
      121 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      181 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      241 ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct atggacttcc
      301 agcactccgg catcgaggaa gtggtgcagg ttttccgcac ggactcctgc tccggccata
      361 atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      421 tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgtaag attattgagt
      481 gtatttaaag ggttagcgca catatcgaag gcacgatgaa aagtcactgc cagcggaccg
      541 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaacg
      601 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcttc aagaatggcg
      661 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcggataat cggatgcact
      721 gggatcgtca cccgctggcg cacggatttc agtaccccca acgacggcgt taagcccccc
      781 tcttttggag ctgcgcataa ttcaactctg tcagcgccgt tttgctgcgc cgttagcgcg
      841 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta gttacgccga
      901 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatgg ttgttttacc
      961 atcggtgacc gccagcgtcg gattcggtaa tcgttcatgc tcaccttttg gcgaactggt
     1021 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1081 attcagcgtt tctggatcac ccggtagaac gatttcaata tgttcccaac cttcgtgcgg
     1141 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1201 tagcggttca tgcaatttaa acagacaaat cggtctgcca ttgatcatat tttctgacaa
     1261 aagctcgccg cactgttcaa acccgcgacg ccagcgttca gcggtgacgt tttgatggca
     1321 acgcaaggaa atgtgatcgg cagtcagcgg agtgatatcc aaccccagac ggcgggaaag
     1381 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1441 agattgccag ttcgccataa tcactcttcg tctttcatta aaagcgctaa tttaccctgt
     1501 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatggt gaaaacggca
     1561 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1621 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1681 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1741 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caggctaacg
     1801 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1861 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     1921 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1981 atcgtctcgg tgttgctacg ccagaaaaac aaaccattgt ggttgactac tctgcgccaa
     2041 acgtggcgaa agagatgcat gtcggtcacc tgcgctctac cattattggt gacgcagcag
     2101 ttcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2161 gcactcagtt cggtatgctg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2221 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2281 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2341 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2401 acgatcgtct gaacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2461 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2521 gcgcgaccgt tgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2581 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2641 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2701 aacacctgat gcaggcgtgg gctatcgtcc gcaaagcagg ctatgtaccg gaatccgtac
     2761 cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg ttcaaaaccc
     2821 gcgcgggtgg tacagtgaaa ctggcagacc tgctggatga agccctggag cgtgcacgcc
     2881 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2941 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     3001 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3061 cttacacgcg tgtattatcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3121 cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3181 ttgaagaaac cctcactgtg gttgcccgtg aaggcacgcc gcatgtgatg tgtgcttacc
     3241 tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc ctcagcgcag
     3301 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3361 agctgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3421 caacagccga tgtcattgca gacatcggct tttttatcat taacgataat ttacaatcac
     3481 ttcattccgt tgaatcttta gcggcggaat taaccgccca ccgccaggca cttcccagat
     3541 aaagcgcaac ggttctgccg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3601 ctctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3661 accgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3721 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3781 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3841 taaagttctc atcatttacc accaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3901 agttccagat tcgacagcac cactaactgc ggcaagctgc ggcgcaggaa gcgagacaat
     3961 aatggtcgca gcgcgtggtt caccaacagt accggcggcg cacccaacat ctcctgacgg
     4021 gatagcgctt cctgagtttg cgccaacaag cgatccgcca gccctggctc cagtcccccc
     4081 ccgccctgca atgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatgg
     4141 acttcatctt tgccaggaaa ccactgctgg gtaatcgcac gtcccaacgc cacgcgcacg
     4201 acagcggtta attcatgcgg atcgctctgg atgggcgcat gttccgccag cgtttcgaga
     4261 atggtgcgca tatcgcgaat cggcactttt tcatcgagca gattttgcag cactttatgc
     4321 agtgtggtaa gcgtgacgac gccaggaacg agatcttccg tcagctttgg catctcctgg
     4381 gcgacgcgat ccagcagctg ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg
     4441 ctaatgaggt ggttaagatg cgttgccacc accgtgctgg cctcaaccac ggtgtacccc
     4501 tgaatctgcg cctgttcttt aagcgcactt tcaatccaga tagcattcag gccaaatgcc
     4561 ggatcaacgg tcgcttcacc aggtaatgtt ccggcggcag tgcctggatt aattgccagc
     4621 cagcgccccg gataagcatc accactcccc atttcgacac ctttcatcaa gatacgatag
     4681 cgggctggtt gcaaatccat attgtcgcga atgtgtacca ctggcggcag aaaccccatc
     4741 tcctgggcaa atttcttgcg gatactgcgt atacggccca acaactcacc atcctgctgg
     4801 aaatcgacca tcgggatcag tcgataaccc acttccattc ccagagaatc ttccagttgt
     4861 acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac cggttttggt
     4921 tcagcaggcg ctttttgttc acgtccgcgg atccaccagg ccagccccag caatccggca
     4981 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     5041 gcggcgctta ataacataac gctcggatta ctgaaaagct gattcaccat ctgctcgcca
     5101 acatcctgat cggtgctgac acgtgtaacg atgacgcccg cggcggtaga aatcaccagt
     5161 gccgggattt gtgccaccag accgtcgcca atggtcaata gcgtataact ttccgccgcg
     5221 tgccccatgc tcatgccatg ttgcagcaca ccgaccagca atccgccgac aacgttaatg
     5281 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatc
     5341 gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc ttcatcttca
     5401 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttacccgg cataccatcg
     5461 agaacaaagc gcgcgcccac ttccgcaata cgcccggcac ctttggtgat aaccataaag
     5521 ttgatgatca cgagaatgac gaacaccacg ataccgatag cgaaattgcc accaacgagg
     5581 aagtgaccga acgcttcgac cacctttcca gccgccgccg cgccggtatg cccttccatc
     5641 aaaatgatac gggttgaagc cacgttaagt gccagacgca acagcgtggt aaacaacagg
     5701 atggtcggaa acgcagcaaa ctcgagcgtg cgctgggtaa acatcgccac cagcaacacc
     5761 atgatcgaca aggcaatatt gaaggtaaac agcaggtcga gtatgaatgc gggcagtggc
     5821 agtaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccattgt
     5881 gtcgatttca ggtttgcagg caggcgcagc atcgcggcca gattactcat gggtcggttt
     5941 ctcgttaata aaatccaagg cttccggcac cggaagatga gtaggttgta cagggcgctg
     6001 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6061 ggcgtacagt tgacccggga tttgttgacc gatctccgca tgtcgataca gcgcacgcgc
     6121 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     6181 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     6241 ctgcaacgct accgaatagt gggtcgggtt attgacaatg acatccgctt tcggcacatc
     6301 ggccatcatc cgacgccgtg cggccgctcg ctgcatctga cggatccgcc ctttaacatg
     6361 ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgtgaca ttcgcagctt
     6421 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accattggaa tgacaccaag
     6481 caccaccagc agcgcgcata gccctaccag atccatcgca ttacccatgg cggtaatcgg
     6541 agactcggcc attaagcgca tcatctgcgg ccagtgatgc cagagaaaaa accccgtcac
     6601 gctgccaacc aggatggttt tcaaaattgc cttaagcaac tccgcgccag tctgtgccga
     6661 gaacatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     6721 tccgctaaat accagccccc ccagcataac cggagaaata atcgccacca gcaccacgcc
     6781 gctaatcagc ggtagcagcg ccagcatggc ttctctgatc agcagaataa tctgcccgag
     6841 gatcagattc gggtcattga taatactgtg atcaaaatgc agccctgcgg agagcatgcc
     6901 cgacaatcga cgggccagcg acacaccgcc aaaccagata acactaacgc ccaccagcaa
     6961 aatcagcagt gaagtcagtt cgcgggaacg cgggatttgc ccctcttccc gcgctttttc
     7021 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7081 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agtgttcaat ggtttgagta
     7141 aggggcaaaa caggcgggat ttagggcttt tgccacagca catcaggcat ggtgacatgt
     7201 ttgtcgggta cggcgtaaac gccttatcca accttgaaga tctgatgtga cgcggccacg
     7261 tcacatcagg caatacaaat caaaatccaa gactatccaa caaatcgtcc acctgatcct
     7321 gactggctac cacaccggct ttgctggtat cgacctgagg tccattaagc aaactctggt
     7381 tttcacgttt tggacgcgac tcctgttccg ggatgttttc caacagcacc atcagcaact
     7441 gacgttcgat ctcctgaatg acatccatca tccgcttaat gacctgtccg gtgagatcct
     7501 gaaaatcctg cgccatcatg atttccagca gttgcgcgtt agtaaagctg gtatgtgcgg
     7561 ggacatctgc cagaaattgc cgcgtgtctg ttaccagttc acgggcgtcg gcaaggtcaa
     7621 tcggatcggc aaaccagtca tcccaacgtt gggttaacgc ttttgctgat ttctccattt
     7681 gatcctgatg cggttgtgac gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg
     7741 tcatctgcac aacatagtac aaacgatcgc gcgcatcggg gatagcttcc gcggcttcgg
     7801 caatggcctg atccagcccc agttcccgca aactgtcgcg cagcatacgc gtcaggctgc
     7861 cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc aggtttgatt gatggttgca
     7921 tcatagtcgc atcctcacat gcccagtttc tcaaagattt tgttgagttt ttcctccagc
     7981 gtcgcggcgg taaatggttt caccacatag ccactggccc ccgcttgcgc cgcagcaatg
     8041 atgttctctt tcttcgcttc tgcagtcacc attaacactg gcaatgccga catcgcgcca
     8101 tccgcacgaa tcgttttcag caactccagg ccatccatat tgggcatgtt ccagtcggag
     8161 ataacaaatc cataaccgcc tgcctgcaac ttattgagag cgtcgaggcc atcttccgct
     8221 tcctcaacat tattgaatcc cagctctttc agcaggttac gcactatgcg tcgcatggtg
     8281 gaaaagtcat ccacaaccaa aaatttaagt tctttatccg ccatttcaca ctcctgattt
     8341 aaatacgtat cgcctgtccg acactaattt ttgccagcat ttgctgactt acctggctaa
     8401 gatcgaccac ttcgcagaca ccacccatat tgatggcctc gcgcggcatg ccgaacacca
     8461 cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc ctgacgcatc gccaacattc
     8521 ccgccgcgcc gtcgttgccc ataccggtta ggattacccc aaccgcatta cgccccgcct
     8581 gtttggccac agaatggaac aacacatcta ccgatggccg atggcgatta accgccgggc
     8641 catcgtgaat tttgatttga taatttgcgc cactacgcgc cagctccata tgccgatcgc
     8701 ccggcgcgat ataggcatgt cccggcaaga cgcgctctcc gtcttcggct tctttaactc
     8761 cgatctggca aagcttatta agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat
     8821 gctgggtaat taacagtgcc ggactggaaa gcggcaacgg ttgcagtacg tgacgaattg
     8881 cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc aacaacggcc
     8941 ccgccttcag cgttgtcggt accgacaatg gcttatgtgc tgcaaggctc gcctttgctg
     9001 ccgtacgcac cttttcagca atcatttcgc tatatgccag cattccttcg cgaataccca
     9061 gttgcggttt ggtgacaaaa tctatcgccc ccagctccag cgcgcgcagc gtaacttctg
     9121 accctttgcc ggtcagggaa gaaaccatca caacgggcat tggacgcaaa cgcattaatt
     9181 tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc aatacatcgg
     9241 gattgaattt cttaaccaaa tcacgcgcaa ccagcggatc aggggcggtc gccaccattt
     9301 ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt gccgaatcat
     9361 cgacagataa caccctgatt ttgctcatcg ttaatcctta cttagcgcat acaccgtctg
     9421 accacgcagc atgaagcggc gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag
     9481 caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atcttctgct gggtagtttg
     9541 atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg gcacggtgta
     9601 ctgtttcgcc agtaaattca gcggggcaaa atcaacatag ttcgccagct cctgacgcac
     9661 gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg
     9721 cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt ccagcacttc
     9781 ggtgtcgata tcgctggcaa acactttcca gcgtccgggc gcggtgccca atgtatcagc
     9841 cagcgtcatc gctatgctgt acggctcttc gccggttgaa gccgccgcac tccatacgcg
     9901 atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga ggaaaatggt gtgcctcacg
     9961 gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact caccgctgtg
    10021 ctgattagat tccagcaagt tcaggtaatg accgaaatcc gccagtccca gcgcacgcaa
    10081 acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc
    10141 tcgttgatag atcaattgac ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc
    10201 ggtcatctgt aacaataaag acgtttgccc acagggcaga gatgaagtca tagcgccttc
    10261 tcaatcactt caggatacca ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac
    10321 ttcatgttct tcaagggtaa ataccgccac acgcgacgaa agatggtcgg cctgattcag
    10381 tcgttgcaaa tcgtcgcgaa atgcccaaaa gacatgccgt tactgccaat ctgcaagatc
    10441 ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt aaacatcaac
    10501 gctccccatc aggcggcaat gaccgcttta gtaaatactc gtcaaaatgt ttcccagttt
    10561 ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg gttgctcact ggcaggacga
    10621 gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacggaacgc ggaaaccgct
    10681 tgcgttaaac gactcgcctg ttcttccagc gcagctgcgg cggcagctga ttcctgcacc
    10741 agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc gacttgatcg
    10801 atgccacgac tctgttcatc cgatgctgat gcaatctcgc ccataatgtc agtcacgcga
    10861 gtgacagcat tgacgatatt gttcattgtt tcccctgcgc tttcgaccag caccgaaccg
    10921 gtatcaacgc gcgagacgga gtcttcaatg agggctttga tctcttttgc cgcctgggcg
    10981 ctgcgactgg caagattacg cacttcgccc gccaccacgg caaaaccacg gccttgttca
    11041 cccgctcgcg cggcttcaac agcggcattc agcgcgagga tattagtctg gaaggcaata
    11101 ccatcgataa cgctgataat gtcggcaatt ttcttcgaac tatcggcgat ctcatgcatc
    11161 gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga ggcactttgc
    11221 gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc ggtgagctgc
    11281 tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttcagtacg ggaggaaaga
    11341 tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga accttcgcgc
    11401 acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg cgccaggtcg
    11461 cccatttcac tgcgcccgtc aatggtcagg gtattcgcca ggttaccacc ggcaatttcg
    11521 cgaatatgcg caataatttt tgccagcggt gtaagcaaca tacggcgaat gccgtaccac
    11581 gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca ctgggcaaat
    11641 cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact gctgagggcg
    11701 tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg agcgaaataa
    11761 gctccagtat tgccataatc aagataatca atcagttcag ttaacgctgt gtgatagttt
    11821 ttatattttt catcaatatt acgactggta gcgaccattt caggtaacgg tgccatgctt
    11881 ttgaattttt tataatgagt cgcggcctgg gccaatgttt tcctggcact atcgagcaac
    11941 tcaactttgg cattactttg ctgattggat gaatccatca tcatccgcac cgctgaacga
    12001 ctcaggttaa tgcgcgtttg tagcattaaa tcccatgttg acgtcagctc gccctgctgt
    12061 tcccgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga agaaaaaaac
    12121 agactgccgg aaataagctg taacagtgcg aataccccca gcaccattac caacagcgtg
    12181 actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttgat ctcgttatcg
    12241 gcaaggaggg gggaaacttt attgctgatg ccacccgccg cgaaattgaa ataaaaaacc
    12301 cgatgcgcag atcatcgggt tcatttcaat tgaggacatc gggagaatta cgccacttct
    12361 gacgccgcgc tatctaacag cgccatctct tcgctgttca gcagtttttc gatgttcacc
    12421 agaatcaaca tgcggtcgcc cagtgcgccc agtcctgtga gatattcggt tgaaagcgtc
    12481 acggcaaatt ccggtgccgg acgaatttgc tccgccgtca atgaaagcac gtctgagacg
    12541 ccgtcaacca cgatgccgac cacccgctgt ccgagattca ggacgataac taccgtgttg
    12601 tcgttatagt ccacatccac ctggctgaac ttaattcgta agtcaacaat cggcacaata
    12661 acgccgcgca gattcgtgac gcctttgata aacgctggcg tgttcgcaat ccgtgtcacc
    12721 tgatcgtagc cacggatctc ctgcactttc agaatatcaa taccgtactc ttcatcacca
    12781 agggtaaata ccagaaattc ctggcctgac ggctcgctgg ccagctttgt tacattcgtc
    12841 ataccggtca tattgttacc tttttactca ttcaggcggc ggtgttcgcc atacgttgtt
    12901 cgcggtttat cgcctgcaag gcggagacat caacaatcag tgccacgctg ccgtcgccaa
    12961 gaatggtcgc agcagaaatg ccggggactt tgcgatagtt actttcaagg tttttaacca
    13021 caacctggtg ttgaccaatt aattgatcca ctagcaaggc gtagcggcga ccgccacttt
    13081 gtaagatcac cacaattccc tgggtggctt cggttttcgc gcccgcgacg ttgaacactt
    13141 tccacagttc gacgataggc agatattcac cccgcacttc cagcacccgc tcgtcgccag
    13201 ccagtggatg cagatcggct tcacggggtt gcagtgattc catgacagca ttcagcggca
    13261 gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc aagaattgcc agcgtcagcg
    13321 gcagtaaaat gcggatcgta gtgccagtac cctgcatcga ctggatctcg acatgaccgc
    13381 ccatctcctg gatattacgt ttaacgacgt ccatgccgac gccacgcccg gagacgtcgg
    13441 tgacctgctc cgccgtggag aagccaggtg caaatatcag cattgcaact tcgtcgtcgc
    13501 tcatgttttc gctgacggtc agaccttgcg aggccgcttt tgccagaatt cgttcacggt
    13561 ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt gccgccctga tgttcggcag
    13621 acagaattaa atttccgacg ctgtttttac ctgcagcgag ccgtttttct ggcagttcaa
    13681 taccgtgatc gaggctattg cgtaccaggt gggtcagcgg gtcgataatg cgttcaatca
    13741 ggctcttgtc gagttcagta gaactgccca ccagcgtcag ttctacctgc ttgccgagtt
    13801 ttcccgccag atcacgcacc agccggggat agcgactaaa gacatattcc atcggcatca
    13861 tgcgaatcga catcactgat tcctgcaaat cacgagcgtt acgttgtaac tgccccatgc
    13921 tggtaatcaa atcaccatga ttaaccgggt ccagttcact ggagcgctga gcaagcatgg
    13981 actgggtgat gaccagctcg ccgacgaggt taattaattg atcaaccttt tctaccgcta
    14041 cacggatgct ggtggattca ctgctgcgcg tcgttttttc ccgctccacg cgaccggttg
    14101 gcgcttgttc ggctgccagt ttaagcactg gtggggtgga tatttttggc gagacgtcta
    14161 ctgtttcaaa ggtaatctga ccggcttcaa tcacaaaaca gagtaccgct gtgatgtcat
    14221 cttcggcgat gtcgcccggt aatattgccg agagagaatc cgcccctttc accacgtcag
    14281 ttaacgttgt cagatgcccc aactcttctt ccagcaggtc gacttcgccg gccttcaggc
    14341 gcgaaaggat aattcgtcgc ggtgactgac tacgactctg ctcatcttgc ggttcacttt
    14401 tggcaaccac acttaatcgg gtcactgcag atggcgtttc gcctttcgct tctaatgcca
    14461 gttgacgcaa ggcctggcag atataatcga agctggcggc atccggctct tgcgactgtt
    14521 tataagcgtc gagctgttct tgcatgatgt ccttcgtttc caaaaacaga ttgataatgt
    14581 cggtgttgag ttgcatctca cctcgtctgg cttcatcgag caggttttcc atcagatgcg
    14641 tggtttcctg caaaacgctg aagccaaagg ttcctgcccc tcctttgatc gagtgggcag
    14701 cccgaaagat ggcattcaac tgttcggcat ctggcgcttc cggctgcaaa accagcagat
    14761 gctgctccat atcagccaac agttcgtccg cttcatcaaa aaatgtctga taaaaatcgc
    14821 ttatatccat gctcacgctg tcacctcggt tcggctgatg gcattgtggg aacactgacc
    14881 tgtggtgcaa cctcaggttt ttccagggcg cttactggct cattctggct ttcggcgttt
    14941 tcatgcaaaa tggcctgttc ggcgtgtttg ttcagtacca gcaggctgat gcgacggttg
    15001 atggcatcat caggtccgcg atcgcttaag cgcatcgttg ccgccatgcc gacgacacgt
    15061 aacactttgc cgccattcaa cccgccaacc atcagttcgc ggcgggatgc attggcccga
    15121 tcggcagaaa gctcccagtt gctatatcct ttctcaccac tggcgtaagg gaaatcatcg
    15181 gtatgacccg aaaggctaat acggttggga ataccattca gaacgggcgc gatggcgcgc
    15241 agaatgtcgc gcatataggg ttcgacgtcg gcactgccgg ttctgaacat cgggcgattc
    15301 tggctatcga tgatctgaat acgtagacct tcctggacca gatcgatttt gagatgtgga
    15361 cgtaacgccc gcagtttcgg atcggactct atcaattggt cgagatcgcc acgcaatttc
    15421 cgcaatcgac tttgctccat gcgttttttc agctcttcga tgttcggctg cttattcact
    15481 tccccctggc tttgggtgta atcatcacca ccgccgggaa ttgggctttc actattagaa
    15541 atgcgatcgc cgcccgtaac cgctgtcgcc agcggagtcc ggaagtactc cgcaatctga
    15601 atcagctctt ttggactgga gatagagatc agccacatca ccagaaaaaa ggccatcatt
    15661 gcggtcataa agtcggcata agcaatcttc cacgatccat gtgctgcccc gtggcttttg
    15721 gctttgcgtc gtttgacgac aataatcgga tgcgcttgat tcttcatgct tcctcggttg
    15781 tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc ttccagctca ataaacgacg
    15841 gacgttcgct ggaatagagc gttttgcgac caaactcaac ggcgataggc ggtgcgtaac
    15901 cgttcagatt agaaagcaga gtgactttga cgcactgcat cattttgctg gtttcggcgc
    15961 ttttctgacg taaaacactc gctaatgggg aaataaatcc gtaagccaat aaaataccga
    16021 ggaaagtccc caccatcgca tgagcgataa gcgcccccag ctcggcggca ggacgatcgg
    16081 ctgaacctaa cgcgtgaacg acccccatta cagccgcaac aataccaaac gccggaagtg
    16141 agtccccgac cagcgccaga ctgttcgccg ggacttctgc ctcgctttcg tgcgtctcaa
    16201 tctcttcatc catcagagct tcgatttcga aggtgttcat gtgaccactg ataatcaggc
    16261 gcagataatc gacgataaat tcaagcatga cgctatccgc aaggatgcgt gggtagctgg
    16321 cgaagatctc actctcacgg ggattttcaa tatcgcgttc cagcgaaaac atccccatct
    16381 gccgcgattt cgccatcaac cgataaagca gagccagcag atccatatac attgctttgg
    16441 tgtatttgga gcgacgaaac agcaacggca gcgccttcag cgtgccttta atcgctttgc
    16501 cattattgcc gacgataaac gacccaatcc ctgcaccggc aataatcacc agttcagcgg
    16561 gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa actgtaccga
    16621 gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt tgaccatgac
    16681 aggatgttca gtcgtcaggc gttaacacgc gataggggca aaaaaaagca gcggtacatc
    16741 gttaccgctg ctggaatgtt tcgccacacc gtatcagtta aacagcctgt actctctgtt
    16801 catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt ctttttactg
    16861 cccgggatgg cggctgacat aagctgcagg caaagctgcc aacaggctgg tgagcgtggg
    16921 taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca ctttcaacaa
    16981 accgcaccaa tgtccaggca cgggttaatg ccagcagcgg tccttcttct gcttgcgggc
    17041 actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta cacaagccag
    17101 ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacattt tgttcccagg
    17161 tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt ccgcgcagtt
    17221 ctttatagag ttttatcaga cgtccgcgac ttaactgtgt ttcgctttcc agcatctgca
    17281 aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccgcgct tcctgaacaa
    17341 tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag gctgattaac
    17401 atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga gatcgtcaac
    17461 gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga agtgacaaac
    17521 cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg ctaacgttgt
    17581 cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct gaacaatcag
    17641 acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca gcaactcgga
    17701 ggtatgcatt attcccaccc agaataacca actttatttt tatgcggtca caccgcaccc
    17761 cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt tgcgataagc
    17821 tgcaataagc agacccacct ttttggttta ataagtcctt acaaatagaa gggggtcttt
    17881 acacttatct aagatttttc ctaaatcgac gcaactgtac tcgtcactac acgcacatac
    17941 aacggagggg ggctgcgatt ttcaataatg
//
LOCUS       DAZFRG010000091        17688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFRG010000091 DAZFRG010000000
VERSION     DAZFRG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.091"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..394
                     /locus_tag="ACHKUC_002384"
                     /pseudo
     CDS             59..394
                     /locus_tag="ACHKUC_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            986..1519
                     /locus_tag="ACHKUC_002385"
     CDS             986..1519
                     /locus_tag="ACHKUC_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6618633.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGKHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDWVNTIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1677..1859
                     /locus_tag="ACHKUC_002386"
     CDS             1677..1859
                     /locus_tag="ACHKUC_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618634.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1874..2005
                     /locus_tag="ACHKUC_002387"
     CDS             1874..2005
                     /locus_tag="ACHKUC_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618635.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVALFLSMDWMMLSSSGLLG"
     gene            2008..2475
                     /locus_tag="ACHKUC_002388"
     CDS             2008..2475
                     /locus_tag="ACHKUC_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6618636.1"
                     /translation="MNRMLSAFIVILLVACGALWMATDHYRDNAITYKAQRDNKASEL
                     KLANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPQLADTVTRDYFTLRERLMTMHKQLEGAQDYIRTQCLK"
     gene            2787..3113
                     /locus_tag="ACHKUC_002389"
     CDS             2787..3113
                     /locus_tag="ACHKUC_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO6618637.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            complement(3236..3415)
                     /locus_tag="ACHKUC_002390"
     CDS             complement(3236..3415)
                     /locus_tag="ACHKUC_002390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618638.1"
                     /translation="MVSKSKAHCRRMLQALQQTRAGIFDQLENCQHTLPEYIAISSET
                     SATLIHRVPPEKKKK"
     gene            4058..4606
                     /locus_tag="ACHKUC_002391"
     CDS             4058..4606
                     /locus_tag="ACHKUC_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6618639.1"
                     /translation="MNVNKKKLADIFGVDVRTITAWQSQGLPLVSGGGKGTESVFDTT
                     AAIQWYAQREADIKNEKLRKEIEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPDISPVHLDAVKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            4578..6506
                     /locus_tag="ACHKUC_002392"
     CDS             4578..6506
                     /locus_tag="ACHKUC_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6618640.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVPVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAADNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYC
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            6490..6696
                     /locus_tag="ACHKUC_002393"
     CDS             6490..6696
                     /locus_tag="ACHKUC_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO6618641.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            6693..8285
                     /locus_tag="ACHKUC_002394"
     CDS             6693..8285
                     /locus_tag="ACHKUC_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6618642.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETATK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            8275..9780
                     /locus_tag="ACHKUC_002395"
     CDS             8275..9780
                     /locus_tag="ACHKUC_002395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6618643.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAVQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAATLAAMPE
                     RTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVQGGVSQASEDGDADVKMLMAMP"
     gene            9817..10164
                     /locus_tag="ACHKUC_002396"
     CDS             9817..10164
                     /locus_tag="ACHKUC_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6618644.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     GATGKLVVWDGQKAGSAVGILVLPLEGTEAVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFTGSALSHAALP"
     gene            10222..11250
                     /locus_tag="ACHKUC_002397"
     CDS             10222..11250
                     /locus_tag="ACHKUC_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6618645.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPIVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            11302..11685
                     /gene="gpFI"
                     /locus_tag="ACHKUC_002398"
     CDS             11302..11685
                     /gene="gpFI"
                     /locus_tag="ACHKUC_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6618646.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVMVPSPLATAMIQHGTAEHA"
     gene            11678..12031
                     /locus_tag="ACHKUC_002399"
     CDS             11678..12031
                     /locus_tag="ACHKUC_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6618647.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            12047..12580
                     /locus_tag="ACHKUC_002400"
     CDS             12047..12580
                     /locus_tag="ACHKUC_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618648.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELASALKQQLRIAIKR"
     gene            12577..12972
                     /locus_tag="ACHKUC_002401"
     CDS             12577..12972
                     /locus_tag="ACHKUC_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6618649.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            12980..13726
                     /locus_tag="ACHKUC_002402"
     CDS             12980..13726
                     /locus_tag="ACHKUC_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618650.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDGWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTGTVEKGKTTTLTVTVEPENATDKTFRAISADPSKATISVKDMTITVAGV
                     KDGKVSIPVISGNGQFAAVAEITVNNVPGG"
     gene            13745..14176
                     /locus_tag="ACHKUC_002403"
     CDS             13745..14176
                     /locus_tag="ACHKUC_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6618651.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            14203..14616
                     /locus_tag="ACHKUC_002404"
     CDS             14203..14616
                     /locus_tag="ACHKUC_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6618652.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSVDVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            14597..17158
                     /locus_tag="ACHKUC_002405"
     CDS             14597..17158
                     /locus_tag="ACHKUC_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6618653.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMAKQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGNWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRGFTR
                     SVLSMLTEIFLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGSQLMKAVYDMARKGAQDELRLQLRDGGLLSGSGR"
     gene            17155..17484
                     /locus_tag="ACHKUC_002406"
     CDS             17155..17484
                     /locus_tag="ACHKUC_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618654.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            17484..>17688
                     /locus_tag="ACHKUC_002407"
     CDS             17484..>17688
                     /locus_tag="ACHKUC_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6618655.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEM"
BASE COUNT         4228 a         4073 c         5463 g         3924 t
ORIGIN      
        1 aacctgtatt tcaaaatcaa agaggaccgg cgtaaggcgg cgcggggaga gtaaagtgat
       61 gaataaaaaa tatgaactgg ttgttaaggg gataaataat tacggggata aggttactgt
      121 tactgtgaag ccggaaggtg acgggcaagc gtcgctgttg ttgccagatg tggcgattag
      181 tcttgaccgt actgaaggtg ccacgctgga gttttacgaa gctgaggcga aaaagcaggc
      241 gaagcagttt ttcatggatg ttgctgccgg gttatgtgaa ggggatggtc cgttgccgga
      301 aaagcgcccc gtaattttag aggcgcagga tgtgttgata acctacaaag gaaagctacc
      361 gggaagaatt acttgttctc tgaagatgcc gccgtcaaca ctgcggtcag aaaaagatga
      421 tgttgaatca cgtattgaaa aactggagag ctacgtcgtt gagctgaata agaaatggtc
      481 gatattggtg ccttctggcg atgaaaagca gtttgctgcg tttgacgatt attgtcggaa
      541 agtgatgagc agaaatctcg cagagtgttt cagtattcat aatgataatt tcagtgaccc
      601 ggaatgggag tgtaaccggc catcctttgt tgtatccggt gatgctggga aaataaccat
      661 ctcagaaaat gggaaagtaa cacctccatc gcaccagcac agtgaggagc tcattgaatt
      721 tgccattgat tacctgaaga acaataaaaa gcaggggctg atgaagcgcg ttggccgttg
      781 catgggatat ctgcaggtag ctgctgagat tgaagcgctg gccagtggtg ctgataagga
      841 tgcaattgtg cgggaggctc ttcttcgtga ttttaatact ccacccttta aaaaagtgcc
      901 ggcttactgg cttcatccgg ggctgactta tcttaaagtg cgtatttagt gggccaggga
      961 cagcggctga atatttaata tatccatgaa caccaaaatc aaatacggcc tgtcggctgc
     1021 cgttctggcg ctgattgccg ctggtgcgcc tgcgcctgac attctcgacc agtttctgga
     1081 tgaaaaggaa ggcaagcaca ccacagcata ccgtgatggt gcgggtatct ggaccatctg
     1141 ccgaggtgcc atcatggtgg atggtaagcc tgtgattcct ggcatgaagc tgtcgaagga
     1201 aaaatgcgac tgggttaaca ctatcgaacg ggataaggcg ctggcgtggg tggagaaaaa
     1261 catcagagtg ccactgaccg aaccccagaa agcggggatc gcgtcattct gtccgtacaa
     1321 cattggtccc ggtaagtgtt tcccgtcgac gttttacaga cgaattaatg ctggtgatcg
     1381 aaaaggtgcc tgcgaagcga ttcgctggtg gattaaggac ggtggcagag actgccgtat
     1441 tcgttcaaac aactgttacg gtcaagtatc ccgtcgtgac caggagagcg cgctggcgtg
     1501 ctggggtatc gacagataag cagaatattt tgctgaaaaa atgaggaatg gccacgcggg
     1561 cggataacac gaaatcctgc gaactggcga aacgtaagtg aataaaagta aaaaccccgt
     1621 ttgttggcac caagcgaggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
     1681 cgaagtataa acgaattctg ttgaggttga ctatgaaaaa tggccttgaa ctgaaagcgc
     1741 ctgtaactga tgacatcagc agagcactgg cttttgccat taagtgggtg gcggtcggtg
     1801 ttgctgtgtc cccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
     1861 gggaggatta agcatgtcag acaaactcat aacgctggcg aagatcctct gtgtaattgt
     1921 cggcatttca ttttcactaa tgctggttgc tctttttctt tccatggact ggatgatgtt
     1981 gtcttcgtcg gggttgctgg ggtgaacata aaccgaatgc tttccgcgtt tatcgttatt
     2041 ctgctggtgg cctgtggtgc gctgtggatg gcaacagacc attaccgtga taacgcgatt
     2101 acctacaaag cgcagcgcga taacaaagcc agtgaactga agctggcgaa cgcaaccatt
     2161 actgatatgc aggtgcgcca gcgcgatgtt gctgcgctcg atgcaaaata ctcgagggaa
     2221 ttagccgatg cgagagctga aaatgaaact cttcgcgctg acgttgccgc tggtcgtaag
     2281 cgcctgcgga tcaacgccac ctgtccaggc tccgtgcgtg aagcccccac cacctccggc
     2341 gtggataatg caaccggccc ccaactggca gacaccgtta cacgggatta tttcaccctc
     2401 agagagcggc tgatgacgat gcacaagcaa ctggaagggg cacaggacta tatccgcact
     2461 cagtgcctga aataagtttt gttgatgcgc cgtatcgtcg ctatattccc tcattaacag
     2521 agaccgcagc ccgacaggga gactcctctg cgcgagtgtg cggggataat taaaaacgat
     2581 gcacaccggg ttttttaccg cgttaatgat tcgcgggttt atcccggtgc gatggtggaa
     2641 gaaacaggaa gctgtattac agaaagtgct actactgtat cccgatgcga tgtatgtaat
     2701 gtgagtcaga taatggcaca ggatgtggtg atgtggcagt ctggaacaca ggatatattg
     2761 tcagaataag acccgtagga ataaaaatga aaagacgcct tttactactt tttctgttat
     2821 ctgtcctggc agtgggatgc tcgcagcaaa aagctgatga gccccggcaa ttagtgacgg
     2881 tgtatccacg atatccggaa tatgctgcag caaattatat caaggggctg gttgaggtta
     2941 agttcgatat tggtgctgat gggactgtga cacggatcgt ttttctccgc tcagagcctc
     3001 ataatttgtt tcgtgatgaa gtggtgaagg ccatggcgaa atggcgattt gaaaagaatc
     3061 gcccctgtca gggagtgaag agacaattta tctttacgcc gtcacgtcct tgatgcttcc
     3121 aggtagagag gggctggacg caggagaaaa atgaaagagc cagcggttat atttttgtca
     3181 tggctgacga ggaatgatgg aagaaggcgt tgtatgccac acaacgcctc actgttcatt
     3241 tcttcttttt ctctggtgga acccgatgaa taagagttgc actggtttcc gatgagatgg
     3301 cgatatactc gggcaaagta tgctggcagt tttccaactg gtcaaaaata cctgctctcg
     3361 tctgttgcaa tgcctgcagc atgcggcggc aatgcgcctt gcttttacta accatctttc
     3421 cttcctctat cagtcgctgc gtgaactcat catgtaatac caggtaaatg cggatgttat
     3481 cggttttggc tacgcagcat agtacaaaac ggacaggtgc atcccgggac gggggaggcg
     3541 tcacatgtcc ctgtgatggt tgttccgggt aatgtactgt gtggggcata aaaatgtccg
     3601 ataattttac tttctaccgc agttagttga ttcgttggtc ctggtagcac attgggcgag
     3661 gatttaaatg ccaggcaact gaaggatgat gtcgcaaggg agatagcgag aatatttctg
     3721 attttcattt gatgatgcct ctgtgtgaaa tgacggtaaa cgacgcactt gtgccggcac
     3781 ataatagcaa gcaccataat agatcagatt cgattcttgc tgtaagtgat aattattctc
     3841 gttttcgggt cctttccgtc gatccaacag gttacggggc ggcgacctcg cgggttttcg
     3901 ctatttatga aaatttttag ggaaaaatca gatccgtttt tcttcttttt aactgattga
     3961 ttatcaatag aattttaaaa atataaaagg atctaacaaa ggctgttttt gttagaaaac
     4021 gccattttca gatcctttct ggttcccggg ggagtgtatg aacgtcaata agaaaaaact
     4081 ggccgatatt tttggcgttg atgtcaggac catcaccgcc tggcagagtc aggggttacc
     4141 actagtttct ggtggaggga aagggactga atcagttttt gatacaactg ctgccattca
     4201 gtggtatgcg cagagggaag ctgatattaa aaacgaaaaa ctccgtaaag agatcgagga
     4261 tttgagggct gccagcgaat cagaccttca gcccggcacc attgattacg aacgttaccg
     4321 actgacgaag gcacaggccg atgcacagga gctgaaaaat gctcgtgagg aaggccttgt
     4381 cctcgagacg gagttattta cctacatctt tcagcgagtg gcacagaata tatcagggat
     4441 ccttgtccgt gtccctcaga cactgcagcg taaataccct gatatatcac ccgtacatct
     4501 tgatgctgtg aaaactgaaa tcgcgaaagc atccgatgtg gcttctgaag ccggtgagaa
     4561 tgtgcgcagg tggattgatg atttcagacg aactgagggc ggctaattct gcaggagcga
     4621 tagcaaccgg cctccttgcg ctaaaaattc ctgtccctct gacgacagtt cagtgggcag
     4681 atcgacatta ttaccttccg aaagagtcat cttacacccc ggggcggtgg gaaacactgc
     4741 cgtttcaggt tgccatcatg aacagcatgg ggaatgaccg gatccgcact gttaatctga
     4801 ttaaatctgc ccgtgttggt tatacaaaga tgttgctggg agtggaggct tattttattg
     4861 agcataaatc acgcaacagc cttcttttcc agcccacgga ttctgctgct gaagatttta
     4921 tgaaatctca tgtggaaccc acgatcaggg atgtgccggt tttactcgat cttgcaccgt
     4981 ggtttgggcg taaacatcgt gataataccc tcacgctgaa acgtttttca tcgggcgtgg
     5041 gcttctggtg cctgggcggg gctgccgcta aaaactaccg tgaaaaatcc gtggacgtgg
     5101 tctgctatga cgaactttcc tcgttcgaac cggatgtcga aaaagagggt tcgccaaccc
     5161 tgcttgggga taaacgtatt gagggctcgg tatggccaaa atccattcgc ggctcgacgc
     5221 ctaaaataaa aggctcctgc cagatcgaaa aagcggccaa cgagtcggcg catttcatgc
     5281 gtttttatgt gccctgccca cactgtgggg aggagcagta tctgaaattt ggcgatgaat
     5341 ccacgccttt tggccttaaa tgggagaaag acagccccga aagcgttttc tacctctgtg
     5401 aacatcatgg ctgcgtgatc catcagtctg agcttgacca gagcaacggg cggtggatct
     5461 gtgaaaacac ggggatgtgg acccgtgacg gtctgacgtt tttcagcgcc gcggataatg
     5521 aaattccgcc gccgcgctcc atcacgttcc atatctggac ggcgtacagt ccgttcacca
     5581 cctgggtaca gatagtctat gactggctgg atgcactgaa agatcccaac ggcctgaaaa
     5641 cctttgtgaa caccacgctg ggcgagacct gggaagaggc cgtgggcgaa aaactcgatc
     5701 accaggtact gatggataag gtcgtgcatt acacggcggc ggtgccagcc cgggtggttt
     5761 atctgacggc gggcattgac tcgcagcgaa accgttttga gatgtatgtc tggggatggg
     5821 caccgggaga ggaagctttt ctggtggata aaatcatcat tatgggccgt cccgatgagg
     5881 aagagacgct gttacgtgtg gatgcggcga tcaacaaaaa atactgccat gcagacggaa
     5941 ccgaaatgac catttcccgt gtctgctggg acaccggggg gatcgatggt gaaattgtct
     6001 atcagaggtc aaaaaaacac ggtgttttcc gggtgctgcc ggtaaaaggt gcatctgttt
     6061 atggcaagcc ggtgatcacc atgccaaaaa cccgcaatca gcggggcgtg tatctgtgcg
     6121 aagtggggac ggacaccgca aaagaaattc tctatgcccg tatgaaagcc gatcccacgc
     6181 ctgcggatga agccacgtcg tatgccatcc gttttcctga tgatccggag attttttcgc
     6241 agacagaggc gcagcaactg gtggcggaag agcttgtgga gaagtgggaa aaaggaaaga
     6301 tgcgtctgct gtgggataac aaaaagcggc gtaacgaagc gctggactgc ctggtgtatg
     6361 cctatgcggc attacgtgtg tccgtgcaac gctggcagct tgatctggct gtactggcaa
     6421 aatcccggga agaagagacg acccggccaa cccttaaaga actggcagcg aagctgtccg
     6481 gaggagtgaa tggttacagt cgctgaacta caggcgctgc gtcaggcgcg ccttgattta
     6541 ttaaccggta aacgggtggt gtctgtccag aaagatggtc gcagaattga atatacggcg
     6601 gcctctctgg atgagcttaa ccgtgcgatc aatgatgctg agttggtact ggggacaacc
     6661 cgccgtcgcc gtcgtccgct gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg
     6721 tgaacggcgt tccgcttcgg gagagcctca gctacaacgg gggcggcgca ggatttggcg
     6781 ggcaaatggc ggagtggttg ccaccggcgc agagtgccga tgcggccctg ctgcccgcgt
     6841 tgcgtctggg gaatgcccgg gcagatgatc tggtgcgcaa taacgggata gcggccaatg
     6901 cggtggccct gcataaggat cacattgtcg ggcatatgtt tcttatcagc taccgcccga
     6961 actggcgctg gctggggatg cgggagaccg cgacaaaaag ttttgtcgat gaggtggagg
     7021 cggcctggtc agaatacgca gaagggatgt ttggtgagat cgacgtggaa gggaaacgca
     7081 cgtttacgga atttatccgt gaaggtgtgg gcgttcatgc gtttaacggc gaaatctttg
     7141 tgcagccggt ctgggatacg gagagcacgc aactgtttcg tacgcgtttt aaagccgtga
     7201 gtccgaaacg ggtggacacg ccaggacacg gtatcgggaa ccgttttctg cgggccggtg
     7261 tggaggttga tcgatatggc cgtgccgttg cgtaccatat ctgtgaggat gattttcctc
     7321 gctccgggag tggacgatgg gaacggatcc cgcgtgaact tcccaccggg cgtccggcca
     7381 tgctgcatat tttcgagccg gtggaggacg ggcagacccg tggggccaac cagttttaca
     7441 gcgtcatgga acggctgaag atgctcgatt ccctgcaggc aacacagctt cagtcggcca
     7501 ttgtcaaggc gatgtatgca gcgacaattg aaagtgaact ggataccgaa aaggcctttg
     7561 aatatatcgc cggtgcgccg caggggcaga aggataatcc gcttattaat attctggaga
     7621 agttctccag ctggtatgac acgaataacg tgacgctggg cggtgtcaaa attccgcacc
     7681 ttttccctgg tgatgatctg aaactacaga cagcgcagga ttcagacaat ggattttctg
     7741 cgcttgaaca ggcgctgctg cggtatatcg ccgccggtct tggcgtttcc tacgaacagt
     7801 tgtcccgtga ttactcgaag gtcagttact caagtgcccg cgcctccgcc aatgagtcgt
     7861 ggcgctattt tatggggcgg cgaaaattta ttgcgtcccg gctggccacg cagatgtttt
     7921 cctgctggct ggaagaggca cttctgcggg ggattatccg tccgccacgg gcacgttttg
     7981 atttttatca ggcgcgttca gcctggtcac gggctgagtg gattggtgcc ggaagaatgg
     8041 ccattgacgg gctcaaggag gttcaggaat cggtgatgcg tattgattcc ggactgagca
     8101 cgtatgagaa agagctggcg ctgatgggcg aggattatca ggacattttc cgccagcagg
     8161 tcagggaatc tgcagagcgg gaaaaagccg gactctcacg tccggtgtgg atagcgcagg
     8221 cgtatcagca gcagatagcg gagagtcgca ggccggaaga ggagacaaca ccacgtgaga
     8281 cgtaatcttt cacacattat tgcagcagca ttcaatgaac cgctgcttct ggagcccgcc
     8341 tatgcgcggg ttttcttttg cgcgctcggg cgcgagatgg gggcagcaag tctttcggta
     8401 ccgcaacagc aggtacagct tgatgctccc gggatgctgg ctgaaacgga cgagtacatg
     8461 gccggaggta aacgaccggc ccgtgtttac cgggtggtga acggtattgc ggtactgccg
     8521 gtgaccggca cgctggtgca ccggctgggg gggatgcggc cattttccgg aatgactggc
     8581 tatgacggca ttgtcgcctg tcttcagcag gcaatggcag atagccaggt gcggggcata
     8641 ctgctggaca ttgacagtcc gggcgggcag gccgccggcg cgtttgactg cgctgacatg
     8701 atttaccgcc tccggcagca gaagccggtc tgggcactgt gcaatgacac ggcctgttct
     8761 gcagccatgc tgctggcgtc ggcctgctcc cgacggctgg ttacccagac atcccgtatc
     8821 ggctccattg gcgtgatgat gagccatgtc agctatgccg gtcatctggc gcaggccggt
     8881 gtggatatca cgctgattta tgccggggcg cacaaggtgg atggcaatca gtttgaagcg
     8941 ttgccggcag aggttcgcca ggacatgcag cagcggattg atgcggcgcg ccggatgttt
     9001 gccgaaaaag tggcgatgta taccggtctg tctgttgatg ccgtcacggg aacagaggct
     9061 gccgtttttg aaggtcagtc cggcattgag gccgggctgg cggatgaatt aatcaatgcg
     9121 tcggatgcca tcagtgtgat ggccacggcg ctgaacagta atgtcagagg aggcactatg
     9181 ccgcaattaa ctgcaacgga agccgccgtg caggagaacc agcgagtgat ggggatcctg
     9241 acatgccagg aagcgaaagg acgtgaacag cttgccacga tgctggcagg gcaacagggc
     9301 atgagcgttg aacaggcccg ggcgattctg gccgcggcgg caccgcagca gccggtggca
     9361 tccgcacaga gtgaagccga tcgcattatg gcgtgtgaag aagcgaacgg tcgtgaacaa
     9421 ctggcagcaa cgctggcggc gatgccggag aggacggtgg aaaaagcccg cccgatcctg
     9481 gctgcttcac cgcaggcgaa tgccgggccc tcacttcgtg atcagatcat ggcactggat
     9541 gaggcaaaag gggctgaggc gcaggctgaa cagctggctg cctgcccggg aatgactgtg
     9601 gagagcgccc gggctgtgct ggctgcggga tcaggtaagg cagaaccggt ctctgcatcc
     9661 acaaccgccc tgtttgaaca tttcatggcg aaccattcac cggctgcggt ccaggggggc
     9721 gtgtcacagg cgtcagaaga cggtgatgcg gacgtgaaaa tgctcatggc catgccatga
     9781 agtcagtgct gaacatcaat acgaggtttt aacaatatgg tgacgaaaac catcactgaa
     9841 cagcgtgcgg aagtacgtat ttttgccggt aatgatccgg ctcacaccgc cacaggcagc
     9901 agcgggattt cctcggcaac accggcactg acacccctga tgctggatgg ggccaccggg
     9961 aaactggtgg tctgggacgg acagaaagcc ggtagtgcgg ttggcatact ggtactgccg
    10021 cttgaaggca cagaggcggt gctgacgtat tacaagtcgg ggacctttgc gacggaggca
    10081 atccgctggc ctgaaagtgt ggatgaacac aaaaaggcca acgcctttac cggcagtgcc
    10141 ctgagtcacg cggcgctgcc gtaacacgtt atcaggccac cgcgttggcc tgactgattt
    10201 cttaatgaaa ggaactgatt tatgggattg tttacgaccc gccagttact cggttatacc
    10261 gaacaaaaag tgaaattccg tgcgctgttt ctggagctgt ttttccgccg tacggtgaat
    10321 ttccacaccg aagaggtgat gctggacaaa attaccggaa aaacgccggt ggcggcctat
    10381 gtctccccga tcgttgaagg aaaagtgctt cgccatcgcg gtggtgaaac ccgcgtgtta
    10441 cgtccgggct acgtcaagcc caaacacgaa tttaattacc agcaggcggt tgagcgcctt
    10501 cctggtgaag atccggctca gctgaacgac ccggcctacc gtcgtctgcg tatcattacc
    10561 gataacctca aacaggaaga gcacgccatt gtccaggtgg aagaaatgca ggcggtgaat
    10621 gccgtgctgt atggcaaata caccatggaa ggggatcagt ttgatactgt cgaggtggat
    10681 ttcgggcgct ctgaaggaaa taacattgag caggctgacg gtaaaaaatg gtctgagcag
    10741 gaccgtgata cgtttgatcc gacgcatgat attgacctct actgcgatca ggccagcggc
    10801 cttgtgaata tcgccattat ggacggtacg gtctggcgtc tgctgaatgg ctttaagctg
    10861 ttccgcgaaa aactggatac ccgtcgcggc tcaaattcac aactcgaaac ggcagtgaaa
    10921 gacctggggg cggtggtgtc cttcaagggg tattacggcg atctggccat tgtggtggcg
    10981 aaaacgtctt atgtggcaga ggacggtacc gaaaaacgtt atctgccgga gggcacactg
    11041 gtcctgggga atacggcagc agagggcatt cgttgctatg gtgccattca ggatgcgcag
    11101 gcgttgtccg aaggtgtggt ggcctcttcc cgttatccga aacactggct gaccgtgggc
    11161 gatccggccc gtgaattcac catgacgcag tccgcaccgc tgatggtgct gccggatccg
    11221 gatgagtttg tggtggtaca ggtgaaataa tccgtgagcg ggggcgaaat gcccccgtgt
    11281 cttttttcac aggaggctga gatggcaaca aaagaagaaa atctgaatcg tcttcgtcaa
    11341 ctggctggcc tgctggggcg cgaggcggat atgtcgggga gtgctgcgga tattgctcaa
    11401 cgtgtgtctg agtgggaaga ggagcttgct gtttccccgg agggcattat gcactctgat
    11461 gagagcgggg ctgatcaaaa tcacacagac gatggtgagc agttgcacaa cactgatgct
    11521 acggatgatg ttaaagcggt ccgtgtgcgg aaatgcctgc atgtgatggg gtattgcccg
    11581 gagacaggcc gtcccgttga actgacgtac cggggcatgc gtgttatggt gccatcacca
    11641 ctggcgacag ccatgataca gcacggaacg gctgagcatg cgtgattttc agaatgcctt
    11701 tgatgctgcc cttgccgggg tggacagcac gattgttgaa gtgatgggga tccgtgcgca
    11761 gttcacctcc ggagcacaac gtggcggcga agttcagggg gtttttgacg atccggagtc
    11821 gctgggtttt gccggtggcg gggtccgtat tgaaggaagc agcccgtcat tatttgtgcg
    11881 gacggatacg gtgcgtgccg tgcggcgtgg tgacacgctg accattaacg gcgagatgtt
    11941 ctgggtggat cgtgtttctc cggatgacgg gggaagctgt tatctctggc tcaaccgtgg
    12001 gcaaccaccc gctgttaacc ggcgacgata aacgcagggt gaaattatgg cgataaaagg
    12061 gcttgatcag gcgattgata atctgagccg ggttcgtaaa aacgccattc cggcggcttc
    12121 agcaatggcc attaaccgcg tggccacaac ggcgattaat cagtcttcat cacaggttgc
    12181 ccgggagaca aaggtacgcc ggaaactggt aaaggaacgg tccagactga aacgggcgac
    12241 ggtcagaaat ccgaacgcaa aaattatcgt taaccgcggt gatcttccag tgattaagct
    12301 ggggatcagg atgctgggcc gtcgtccgaa cagcatactt aaagccggtc agcatcggta
    12361 tcagcgggca ttcattcagc gattaaaaaa cggtcgctgg catgtcatgc agcgtgtggc
    12421 cgggaaaaac cgttacccta ttgatgtggt gaaaatcccg atggcggccc cactgaaaca
    12481 ggcgtttgat gagaatgttg accgtatccg gcgtgaacgc ctgcccggag aactggcatc
    12541 cgcgctgaaa caacaactga ggattgcgat aaaacgatga aacacactga cattcgtgcc
    12601 gcagtgctgg atgcactcga gcagcatgaa cacggggcga cgctgtttga tggtcgcccc
    12661 gttgtttttg acgaagagga ttttcctgcg atcgcggttt atctgacgga tgcagagtat
    12721 accggtgaag agctggatgc agatacctgg cgggccacgc tgcatattga ggtgttttta
    12781 ccggcacagg taccggattc agagcttgat cagtggatgg aaagccggat ttacccggcg
    12841 atgaccgcga tcccggcact ggcaggactg attaccacga tggttacgca gggctatgag
    12901 tatcgtcgtg atgacgatat ggcgttatgg agttctgcag atctgactta ttccattaca
    12961 tacgagatgt gaggacgata tggcaacacc aaatcccctt gagccggtaa aaggtgccgg
    13021 taccactctg tgggtttaca acggcaaggc tgatgcttat gcaaacccgt tgtcagacga
    13081 tggctggcag cgactggcta aggtgaagga tctgacgccg ggcgagatga cggctgaacc
    13141 ctacgatgat aactacctgg atgatgaaga cgcggactgg accgcgaccg ggcagggaca
    13201 gaaatctgca ggtgatacca gttttacgct ggcctggaaa ccgggagagg aaggccagaa
    13261 agggcttata ggctggtttg aaagcggcga tgtccgggcc tataaaatcc gttttccgaa
    13321 tggcacggtg gatgtgtttc gtggctgggt cagcagtatc ggtaaggccg tgacggcgaa
    13381 agaagtgatc acccgcacgg tgaaagtcac taacgtgggt aaaccttctg tagcggaaga
    13441 acgcagcaaa attacgccgg tcactgcgat taaggtgacg ccgacaggta cggttgaaaa
    13501 agggaaaaca accaccctga ccgttactgt ggaaccggaa aatgcaacgg ataagacatt
    13561 cagggcgatt tccgccgatc catcaaaagc caccattagc gtgaaagata tgacgattac
    13621 tgtggcgggg gttaaggatg gaaaagtcag catccctgtg atttccggta atggtcagtt
    13681 tgctgcggtg gctgaaatta ccgttaataa tgtgccgggt ggctaaagag ctgagagata
    13741 agcgatgttc ctgaaaacag aacaatttga atataacggt gtatccgtca cgctttctga
    13801 gctgtctgcg ctgcagcgta ttgagcatct tgccctcctg aaacggcggg cagaagaggc
    13861 tgaagccagc ggcaacctgc aggtgagtgt ggaagatctt gtcagaaccg gcgcgtttct
    13921 ggtggcgatg tccctgtggc ataaccatcc acagaaaacg cagtcaccgt caatgaatga
    13981 ggccgtgatg aagatagagc aggaagtgct caccacctgg cctgccgatg ccattgcccg
    14041 ggcggaagac gtggtgttgt gcctgtccgg gatgatcgaa gctgttcgtc cggatactga
    14101 tattactgaa gtggcgaaaa ataacacgct gactgatgat gatttttctg cgggaaagtc
    14161 ttcgacggcg agctgaactt tgccctcaga ctggcgcgtg agatggggag acccgactgg
    14221 cgcgccatgc ttgccgggat gacatccacc gaatatgccg actggcaccg tttttaccgc
    14281 acgcattatt ttcaggatac ccagctggat atgcattttt ccgggctgac gtacgctgta
    14341 ctcagcctgt ttttttgcga tccggatatg catccctctg atttcagtct gcttgtcccc
    14401 cggcatgagg aagagcaggt ggagaggccg gatgaggaca aaatgctgat gcagaaagcg
    14461 gcaggacttg ccggaggcgt ccggttcggt ggggacggag ggcgcgatat tttatcgtct
    14521 gtggatgtgg cggatgtcat ggtggatgat gccgcattaa tgatggcttc agcggggatt
    14581 ccgggaggtg tgagatatgt cccagccggt tggtgatctt gttattgacc tgagtctgga
    14641 tgctgtccgt ttcgatgagc agatgagccg ggtaaggcgt catttttcag gtctggatac
    14701 cgacgccaga aaaaccgcca gtgctgttga acagggcctg agccgccagg cgctggctgc
    14761 acaaaaagcc gggatttccg tcgggcagta taaagcggcc atgcgaaccc tgcccgcaca
    14821 gtttacggat atcgccacgc agcttgccgg tggtcagaat ccctggctga tcctgctgca
    14881 acagggcggt caggtgaagg actccttcgg cgggatgatc cccatgttca gggggcttgc
    14941 cggtgcgatc accctgccga tggtcggggt cacctcgctg gcggtggcga caggtgcgct
    15001 ggtgtacgcc tggtaccagg gagattccac gctttcagcg tttaataaaa ccctggttct
    15061 ttccggtaat cagtccggac tgactgccga tcgcatgctg acgctctcca gagccggaca
    15121 ggccgcaggg ctgacgttta accaggcgag tgagtcactg gcagccctgg tgaatgccgg
    15181 tgtgcgtggt ggtgaacagt ttgatgccat caaccagagt gtcgcgcgtt ttgcttctgc
    15241 atccggtgtg gaggtggaca aggttgcaga ggctttcgga aaactgacca ctgacccgac
    15301 gtcggggctg attgcgatgg cgaagcagtt ccataacgtg acggcggagc agattgcgta
    15361 tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg cgaacgaggc
    15421 cgcaacgaaa gggtttgatg accagacccg acgcttgaaa gagaacatgg gcacgctgga
    15481 aacctgggca gacaggacag cacgggcatt caaatccatg tgggatgcgg tgctggatat
    15541 tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggccgcgt ttaagaaagc
    15601 ggacgatatc tggaatctgc gcaaggatga ttattttgtt aacgatgaag cgcgggcgcg
    15661 ttactgggat gatcgtgaaa aggcccgtct tgcgcttgaa gccgcccgaa agaaggctga
    15721 gcagcagagt caacaggaca aaaatgcgca gcagcagagc gataccgaag cgtcacggct
    15781 gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc tggagaaata
    15841 taccgcccgt caggaagaac tgaacaaggc actgaaagac gggaaaatcc tgcaggcgga
    15901 ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga aaaagccgaa
    15961 acagtccggc gtgaaggtgt ctgcgggcga tcgtcaggaa gacagtgctc atgctgccct
    16021 gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcaa atgagaaaat
    16081 cagccagcag cgccgggatt tgtggaaggc ggagagtcag ttcgcggtac tggaggaggc
    16141 ggcgcaacgt cgccagctgt ctgcacagga gaaatccctg ctggcgcata aagatgagac
    16201 gctggagtac aaacgccagc tggctgcact tggcgacaag gttacgtatc aggagcgcct
    16261 gaacgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa aacgggccgc
    16321 cattgatgcg aaaagccggg ggctgactga ccggcaggca gaacgggaag ccacggaaca
    16381 gcgcctgaag gaacagtatg gcgataatcc gctggcgctg aataacgtca tgtcagagca
    16441 gaaaaagacc tgggcggctg aagacctgct tcgcgggaac tggatggcag gcctcaggtc
    16501 cggctggagt gagtgggaag agagtgccac ggacagtatg tcgcaggtaa aaagtgcagc
    16561 cacgcagacc tttgacggta ttgcacagaa tatggcagcg atgctgaccg gcagcgaaca
    16621 gaactggcgt ggtttcaccc gttctgtgct gtccatgctg acagagattt ttctgaagca
    16681 ggcgatggtg gggattgtcg ggagtatcgg cagcgctatt ggcggggctg ttggtggcgg
    16741 cgcatccgcg tcaggcggta cagccattca ggccgctgcg gcgaaattcc attttgcaac
    16801 cggaggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc accgtggtga
    16861 atttgtcttc acgaaggagg caaccagccg gattggtgtc ggcaacctgt atcgtctgat
    16921 gcgcgggtat gcggaaggtg gttatgtcgg cggtgccgga agtccggcgc agatgcggcg
    16981 ggccgaaggt attaatttta atcagaacaa tcacgtggtg attcagaacg acggtatcaa
    17041 cggacaggcg gggtcgcagc tgatgaaggc ggtgtatgac atggcccgca agggggcaca
    17101 ggatgaactc cggctgcagt tgcgtgatgg cggtctgtta tcagggagcg gtcgatgaaa
    17161 acctttcgct ggaaagtgaa gccggatatg gaggtgaact cgcagccatc ggtgcgtgaa
    17221 gtgcgttttg gtgacgggta ctcacagcgt atggcggcag ggctgaatgc tgacctgaaa
    17281 acatacaggg tgacgctttc cgtgacccgg gaggaggccc ggcatctgga agcgttcctg
    17341 gcagagcacg gtggctggaa ggcatttttg tggaagccac cctatgcata ccggcagata
    17401 aaggtgacct gtgccgggtg gtctgcgcgg gtcgggatgt tgcgcgttga gttcagcgcg
    17461 gagtttaagc aggtggtgaa ctgatgcagg atattcgcga agaaagtctg aacgagtcgg
    17521 ttaagtcaga gcagtcaccg cgggtggtac tctgggaaat cgacctgacg gtacagggtg
    17581 gtgagcggta ttttttctgt aatgagctga atgaaaaagg ggagccggtc acctggcagg
    17641 ggcgtaagta tgaggcatac ccgattgacg gcagcggctt tgagatga
//
LOCUS       DAZFRG010000092        17608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFRG010000092 DAZFRG010000000
VERSION     DAZFRG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.092"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..188)
                     /locus_tag="ACHKUC_002408"
     CDS             complement(<1..188)
                     /locus_tag="ACHKUC_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFO6618656.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLER"
     gene            405..1436
                     /gene="idnD"
                     /locus_tag="ACHKUC_002409"
     CDS             405..1436
                     /gene="idnD"
                     /locus_tag="ACHKUC_002409"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO6618657.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSKLHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            1460..2224
                     /gene="idnO"
                     /locus_tag="ACHKUC_002410"
     CDS             1460..2224
                     /gene="idnO"
                     /locus_tag="ACHKUC_002410"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO6618658.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            2289..3608
                     /gene="idnT"
                     /locus_tag="ACHKUC_002411"
     CDS             2289..3608
                     /gene="idnT"
                     /locus_tag="ACHKUC_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO6618659.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            3675..4673
                     /gene="idnR"
                     /locus_tag="ACHKUC_002412"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             3675..4673
                     /gene="idnR"
                     /locus_tag="ACHKUC_002412"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO6618660.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            4751..6253
                     /gene="yjgR"
                     /locus_tag="ACHKUC_002413"
     CDS             4751..6253
                     /gene="yjgR"
                     /locus_tag="ACHKUC_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO6618661.1"
                     /translation="MSKPLLIARTLDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAAEGTASEKLLARLKNIGVNDWQPHTNPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILRAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPTFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNTPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(6414..7496)
                     /gene="lptG"
                     /locus_tag="ACHKUC_002414"
     CDS             complement(6414..7496)
                     /gene="lptG"
                     /locus_tag="ACHKUC_002414"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6618662.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7496..8596)
                     /gene="lptF"
                     /locus_tag="ACHKUC_002415"
     CDS             complement(7496..8596)
                     /gene="lptF"
                     /locus_tag="ACHKUC_002415"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6618663.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAINVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(8659..8709)
                     /gene="ytgA"
                     /locus_tag="ACHKUC_002416"
     CDS             complement(8659..8709)
                     /gene="ytgA"
                     /locus_tag="ACHKUC_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO6618664.1"
                     /translation="MSIIILATISTTQENK"
     gene            8863..10374
                     /gene="pepA"
                     /locus_tag="ACHKUC_002417"
     CDS             8863..10374
                     /gene="pepA"
                     /locus_tag="ACHKUC_002417"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO6618665.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10728..11171
                     /gene="holC"
                     /locus_tag="ACHKUC_002418"
     CDS             10728..11171
                     /gene="holC"
                     /locus_tag="ACHKUC_002418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO6618666.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11171..14026
                     /gene="valS"
                     /locus_tag="ACHKUC_002419"
     CDS             11171..14026
                     /gene="valS"
                     /locus_tag="ACHKUC_002419"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO6618667.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTDAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(14082..15278)
                     /gene="yjgN"
                     /locus_tag="ACHKUC_002420"
     CDS             complement(14082..15278)
                     /gene="yjgN"
                     /locus_tag="ACHKUC_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO6618668.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCRRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            15471..15974
                     /gene="yjgM"
                     /locus_tag="ACHKUC_002421"
     CDS             15471..15974
                     /gene="yjgM"
                     /locus_tag="ACHKUC_002421"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6618669.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(16020..16436)
                     /gene="rraB"
                     /locus_tag="ACHKUC_002422"
     CDS             complement(16020..16436)
                     /gene="rraB"
                     /locus_tag="ACHKUC_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO6618670.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            16598..17602
                     /gene="argF"
                     /locus_tag="ACHKUC_002423"
     CDS             16598..17602
                     /gene="argF"
                     /locus_tag="ACHKUC_002423"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO6618671.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         4534 a         4344 c         4518 g         4212 t
ORIGIN      
        1 cgttccagcc agggaagtcg atcttcatca gataatggaa tgccttgcga cattttatct
       61 atatttttgg ctggatgaag atcgtcacca tcaataaatt tcgcagacaa taacgcggca
      121 accttgctac caattaatgt tttaccactc cctgatacgc ccatcaaaat aaagctttca
      181 cccgccattt aaaatctcct gaatcatctt acctgctgcg atactacgcc tggtggcgta
      241 ataatttcac gtttttttat ttctgctgat aagaattaca aggcacatca cgttatgcgt
      301 aacatagtaa tgtaacaatt ttctaacgtg atcttcatca caaataatga caattaaaac
      361 cgcttaaatg cttccgaggt gtctacctga ccagtgagga atttatgcaa gtgaaaacac
      421 agtcctgcgt tgttgcgggc aagaaaactg ttgccgttac cgagcagacg atagattgga
      481 ataataatgg aacattagta caaataaccc gaggtggaat ttgcggttcc gatttacatt
      541 attatcagga aggaaaagta ggtaatttca tgataaaggc accgatggtg ttaggtcatg
      601 aagttatcgg taaagttatt catagcgact catcaaaatt acatgaaggg caaacggtag
      661 ccattaatcc gtctaaaccg tgcggtcact gcaaatactg cattgaacat aacgagaatc
      721 agtgtacaga gatgcgtttt tttggcagtg ccatgtattt ccctcatgtt gatggtggct
      781 ttacccgtta taaaatggtc gaaacgtcgc aatgtgtccc ttatccggcc aaagctgacg
      841 aaaaggttat ggcttttgcc gaacctttag ccgtcgcgat tcatgccgca catcaggccg
      901 gcgagttaca gggcaagcga gtatttattt ctggtgttgg gcccattggc tgcctgattg
      961 tcagtgctgt gaaaacactg ggggccgcgg aaattgtctg tgccgatgtg agtccccgtt
     1021 ccctttcgct gggcaaagag atgggggcgg atgtgctcgt aaacccacaa aacgacgaca
     1081 tggatcactg gaaagcggaa aaaggctatt tcgatgtcag ctttgaagtg tccggtcatc
     1141 cttcatcagt gaatacctgt ctggaggtca ctcgtgcacg cggcgtaatg gtgcaggtag
     1201 gtatgggagg cgcgatggca gaattcccaa tgatgacatt gattggtaag gagatttcac
     1261 tcaaaggctc tttccgtttt accagcgaat ttaataccgc agtgtcatgg ctggcgaatg
     1321 gcgttatcaa tccactgcct ttactgagtg ctgaatatcc cttcacagac ctggaagagg
     1381 cgctacgttt cgccggtgat aaaacccagg cagcaaaagt ccagcttgtt ttctaagaga
     1441 taaataaagg aataatacaa tgaacgatct attttcactg gcaggaaaaa atatcttgat
     1501 taccggttca gcacagggca ttggcttttt actggcgacc ggcctgggta aatatggcgc
     1561 acaaataatt attaatgata ttactgccga acgcgcagaa cttgctgtag aaaaactcca
     1621 ccaggagggt attcaggccg ttgccgcacc ttttaatgtt actcataaac atgaaattga
     1681 tgccgccgtt gaacatatcg aaaaggacat cggccccatt gatgtgctgg tgaataacgc
     1741 cggtatccag cgccgtcatc cttttactga gttccctgaa caagagtgga atgatgtgat
     1801 cgcagtaaac cagaccgccg tgttcctggt gtcgcaagcg gtaactcgtc acatggttga
     1861 acgcaaggca ggtaaagtta ttaatatttg ctcgatgcaa agcgaactgg gacgtgacac
     1921 catcacccct tatgccgcat cgaaaggggc ggtaaaaatg ctcacccgcg gcatgtgcgt
     1981 cgagctggcg cgccacaata ttcaggtcaa cggtattgcg ccgggctatt tcaaaacaga
     2041 aatgactaaa gcactggttg aggacgaagc cttcaccgcc tggttgtgca aacggacccc
     2101 cgccgcacgc tggggagatc cgcaggaact gattggtgct gcggtgttcc tttcttcaaa
     2161 agcctctgat ttcgtaaacg gccacctgtt gtttgttgat ggcggcatgt tagtggctgt
     2221 ttaacagcta ctaccctacg tgcagcgcga aagcgctgct tttctggcac taataagaga
     2281 taaagactat gccattaatc attattgcgg caggcgtcgc gctgcttctt atcctgatga
     2341 tcggctttaa agttaacggc tttattgccc tcgttctggt cgctgccgtc gtcggatttg
     2401 ccgaagggat ggatgcacag gccgtcctgc actctataca aaatggtatc ggcagcacac
     2461 tcggcgggct ggcaatgatc ctcggtttcg gggccatgtt aggcaagctg atttctgata
     2521 cgggcgcggc acaacgtatc gccactacgc tgattgctac ttttggtaaa aaacgcgtgc
     2581 aatgggcgct agtgatcacc ggtctggttg tgggcctcgc catgtttttt gaagtgggtt
     2641 ttgtcctgct gttgccgttg gtatttacca tcgtggcatc atctggatta cccctgttgt
     2701 atgttggcgt accgatggta gcagcgctct ctgtaaccca ctgttttctg ccgccacatc
     2761 cagggcctac tgccatcgcg actatctttg aggctaatct cggaacgact ttactgtatg
     2821 gatttatcat taccattccg acagttattg tcgcaggacc gctgttttct aaactgctaa
     2881 ctcgctttga gaaagcacca ccggaaggct tatttaatcc tcatctgttt agcgaagagg
     2941 agatgccctc cttctggaac agtatttttg ctgcagtgat cccggtcatc ctgatggcta
     3001 tcgccgccgt ttgtgaaatt acgttaccga aaactaacac cgtgcgcctc ttctttgaat
     3061 ttgtcggtaa ccctgccgtt gcgctgttta ttgccattgt tattgcgatt ttcacactgg
     3121 gccgacgtaa tggacgcacc atcgagcaaa tcatggatat cattggggat tctataggcg
     3181 ctatcgcgat gattgtgttt attatcgctg gcggcggcgc gtttaagcag gtattagtag
     3241 atagcggtgt cgggcagtat atttcacact taatgaccgg aactacgctt tcgccgttat
     3301 tgatgtgctg gactgttgcg gcgctgttgc gtatcgctct gggctctgcc accgtcgcgg
     3361 ccattaccac cgcgggtgtg gtgttgccga ttatcaacgt tacccatgcc gatcccgctt
     3421 taatggtact ggcaaccggt gcgggcagcg tgatcgcgtc acacgtaaac gaccctggct
     3481 tctggctatt taaagggtat tttaatctga cggttggtga aacgttgcgt acctggacgg
     3541 tgatggaaac ccttatttct attatgggtt tgctgggcgt gttagccatt aacgccgtat
     3601 tgcactgaaa gaataaaata ttgtgatgta gtttcggggg aagattgaag attcccccga
     3661 cgttgggaga agttatgcgc aatcacagaa tttctttaca ggatatcgct acgctggctg
     3721 gcgtaacaaa aatgaccgtg agtcgttata tccgctcgcc gaaaaaggtg gcaaaggaaa
     3781 caggcgagcg catcgccaaa attatggaag agatcaatta tatccctaat cgcgctcccg
     3841 gcatgttgtt gaatgcacaa agttataccc tcggtatctt gattccgtca tttcaaaatc
     3901 aactcttcgc tgacattctc gcgggaatag aatccgtcac ctctgtgcat aattatcaga
     3961 cccttattgc taattacaat tacgatcgcg attccgaaga agagtcggtg attaacctgc
     4021 tctcttataa cattgacggg attattcttt cggaaaaata tcacaccatc aggacggtga
     4081 aatttctgcg ttcagccacc attccggtcg tggaattgat ggatgtacag ggagaacggc
     4141 tggatatgga ggtcggtttt gataatcggc aggctgcttt tgacatggtg tgtaccatgc
     4201 tggataagcg agtcaggcgc aaaattctgt atctcggttc caaagatgac acccgcgatg
     4261 aacagcgtta tcaggggtat tgcgatgcga tgatgctgca taatctttcc ccattacgca
     4321 tgaatccacg cgccatctca tccattcatt taggaatgca gttaatgcgt gatgccctta
     4381 gcgcaaaccc ggatttagac ggtgtatttt gcaccaatga cgatattgcg atgggtgcgc
     4441 ttctgctgtg ccgcgagcga aacctcgccg tgccagaaca aatttccatt gcgggttttc
     4501 atgggctgga gattggcagg caaatgatcc caagcctcgc cagcgtaatt actccccgtt
     4561 ttgatattgg tcggatggcc gcccaaatgt tgctgagcaa aattaagaat aacgatcaca
     4621 accacaacac tgttgacctc gggtatcaga tttatcacgg taacacgctt taggttattt
     4681 tctcttcatt acacatattc tctttgccgg acgttgccta tactccaggc aggaaactgg
     4741 aggaaatctc atgagcaaac ccctgttaat tgcccgcacg ctggacacag aactgttttt
     4801 actgccggga atggctaacc gtcacgggct gattactggc gcaacgggga caggtaaaac
     4861 tgtcacgctg caaaaactgg cggaatctct gtcagaaatc ggcgttccgg tgtttatggc
     4921 cgatgtaaaa ggtgatttaa ccggggttgc ggcagaaggt acggcgtcgg aaaaactgct
     4981 cgcaaggctt aaaaatatcg gcgtaaatga ctggcaacct catactaatc cggtggtggt
     5041 gtgggatatc tttggcgaga aaggccaccc ggtgcgggcg acggtttcgg atctggggcc
     5101 gctgttgctg gcacgactgt tgaatctcaa cgatgtgcaa tctggcgtgc tgaatattat
     5161 cttccgcatt gcggacgatc agggattgtt gctgctcgac tttaaagatt tgcgggcgat
     5221 tacccagtac atcggcgata acgccaaatc cttccagaat cagtacggaa atatcagtag
     5281 cgcatcggtt ggtgccatcc agcgcggatt actgtcgctg gaacagcaag gcgcagcaca
     5341 cttctttggt gagccgatgc tggatatcaa agactggatg cgcaccgatg ccaacggtaa
     5401 aggcgttatc aatatccttc gcgccgaaaa actctaccag atgccgaaac tgtacgccgc
     5461 cagcctgctg tggatgcttt cagagttata tgaacaattg ccggaagcgg gcgatctgga
     5521 gaaaccaaaa ctggtgtttt tcttcgacga agcgcatctg ctgtttaacg atgcaccgca
     5581 ggtactgctg gataagattg agcaggtgat aaggcttatt cgctcaaaag gcgtgggcgt
     5641 ctggttcgtt tcgcaaaacc cgtcagatat cccggataac gtgctcgggc agctcggtaa
     5701 tcgcgttcaa cacgctttgc gtgcttttac gcctaaagat cagaaagcag tgaaggcagc
     5761 ggcgcaaacc atgcgggcca atccgacgtt tgataccgaa aaggcaatcc aggaactggg
     5821 gacgggcgaa gcgttaatct cgtttctcga tgcgaaagga agtccttctg tggtggaacg
     5881 ggcgatggtg attgcgcctt gttcgcggat ggggccggtg acggaagatg agcgcaatgg
     5941 cttgattaat cactccccgg tgtatggcaa gtatgaagat gacgtggacc gggaatccgc
     6001 ctatgagatg ttgcaaaaag gctttcaggc cagtatcgag cagcaaaata atactcccgc
     6061 gaaaggaaaa gaggtggcgg tggatgacgg tattctcggt ggattgaaag atattttgtt
     6121 cggcactacc ggaccacgcg gcgggaagaa agatggtgtg gtgcaaacaa tggcaaaaag
     6181 cgccgcccgc caggtgacga atcagattgt gcgcgggatg ttggggagtt tgctgggggg
     6241 gagaagaagg taagccaggt gcgatttgtc tttgcctgat gcgacgctgg cgcgccttat
     6301 catgccaaca ccactgcaat atattgaatt ttcatgattt ttcagacatg aacgaagcac
     6361 actttgctaa caataataaa gggagctttc gctcccttta ttcgttcatc aggttacgat
     6421 tttctcatta acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg
     6481 atgatcggcg ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag
     6541 aagacaaaac cgaagctaat accagtgacc acacgcacgc ccatcggtac gctacgcagt
     6601 gggccaaaga tgaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag
     6661 attttgctcc acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac
     6721 ttcacatagt tgtgcaaacc gctgatagag agtgcatccg ggtccagcgc caccacgccc
     6781 agtttgtctg gcgtgaggtt ggttttccag gtgccgctca ccgtctgcga accggtgatc
     6841 tgtttcggat tggtcaaatc agattcatca acctgcgaca gacgccagac tttatgttcc
     6901 gggtcaaact tcgcggtagc ggcatagcgt acggattgca gacgacgatt ctcgttaaag
     6961 gcataaatac tgatgccacc taactcttcg tcacctttaa cccgctcaat gtagacgaag
     7021 ttgttgccat ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc
     7081 gcctgcgcac ggtagttacg cgccatctgc tcgccctgcg gcgcgaccca ttcaccaatc
     7141 gccatcgtca gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata
     7201 cgggtaaaac cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga
     7261 ccaagcaacg ccccaagcag agccgccatc gggaagaaga tttgcacatc tttcggcacg
     7321 ctcagcaagg tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc
     7381 agctgatcga caaacttgat aatgccggac agcgacacca gcatgaacag cgtcatcatg
     7441 atggtggtga aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca
     7501 ccgctccttt acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt
     7561 tgagaacaat cgccaaagcc agataaatca ggttaacggt ccacatccac agcgtcgggt
     7621 ccagcttacc tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat
     7681 acagcagcat ggctggcagc atcgacagta cgcgtccctg acgtgggtta accacgctca
     7741 gcggtacgac cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca
     7801 gttctgcgcg agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg
     7861 tatcgttcgg gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat
     7921 ccgtaatgcg gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag
     7981 tgacgacctg ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag
     8041 aaggacgtgc attacctttt ggtcgaattt gcgcgaggaa cacatctttg aaatcgctgc
     8101 cgtcaacgct ttcgatgaac agcaccgaac tgccattagt cgcttgctgg aattgccctt
     8161 gcgccagcgc cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac
     8221 gcgatgacca cggtcccgcc cacatcacgt taatcgccgc gacgattgcc gtgaataccg
     8281 caaggatcat tgccgctttc accagaaccg ctttgctcag gccgcaggca tgcattaccg
     8341 taatttcact ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta
     8401 atggcaggat aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat
     8461 tcgccggaat atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga
     8521 agatcaaaag caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat
     8581 atcttatgat tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct
     8641 tgtttcatgg cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat
     8701 tatactcaac ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt
     8761 aagattaaca cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc
     8821 tgtagccacc gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa
     8881 agcggtagcc cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt
     8941 cgcctttctc cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg
     9001 ctacgtcggg gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg
     9061 aatgtacttt ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag
     9121 cgtcagtaca agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcaatg
     9181 gaagcggtct gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg
     9241 cgtcaggctg tcgagacggc aaaagagacg ctctacagtt tcgatcagct gaaaacgaac
     9301 aagagcgaac cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa
     9361 ctgaccagcg gtgagcgcgc gatccagcac ggtctggcga ttgccgccgg gattaaagca
     9421 gcgaaagatc tcggcaatat gccgccgaat atctgtaacg ccgcttacct cgcttcacaa
     9481 gcgcgccagc tggctgacag ctacagcaag aatgttatca cccgcgttat cggcgaacag
     9541 cagatgaaag agctggggat gcattcctat ctggcggtcg gtcagggttc gcaaaacgaa
     9601 tcgctgatgt cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg
     9661 ctggtgggta aaggtttaac cttcgactcc ggcggtatct cgatcaagcc ttcagaaggc
     9721 atggatgaga tgaagtacga tatgtgcggt gcggcagcgg tttacggcgt gatgcgtatg
     9781 gtcgcggagc tacaactgcc gattaacgtt atcggcgtgt tggcaggctg cgaaaacatg
     9841 cctggcgggc gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt
     9901 gaagtgctga ataccgacgc tgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt
     9961 gagcgttttg agccggaagc ggtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc
    10021 gcgctgggtc accacattac tggtctgatg gcgaaccata acccactggc ccatgaactg
    10081 attgccgcgt ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtac
    10141 caggaacagc tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg
    10201 gcgattaccg caggttgctt cctgtcacgc tttacccgta agtacaactg ggcgcacctg
    10261 gatatcgcgg gaaccgcctg gcgctctggt aaagcgaaag gcgcaaccgg tcgtccggta
    10321 gcgttgctgg cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaattgcgt
    10381 caggcaaggc tgttattgcc ggatgcggcg tgaacgcctt atccgaccta cacagcactg
    10441 aactcgtagg cctgataaga cgcgcaagcg tcgcatcagg caatgctgtt attgtcggat
    10501 gcggcatgaa cgccttatcc gacctacaca gcaccgaact cgtaggcctg ataagacgcg
    10561 gcagcgtcgc atcaggcaat gctgttattg tcggatgcgg cgtgagcgcc ttatccgacc
    10621 tacacacagc actgaactcg taggcctgat aagacacaac agcgtcgcat caggcgctgc
    10681 ggtgtatacc tgatgcgtat ttaaatccac cacaagaagc cccatttatg aaaaacgcga
    10741 cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt gagcaactgg
    10801 tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtgctcatc gcctgtgaag
    10861 atgaaaagca ggcttaccgg ctggatgaag ccctatgggc gcgtccggca gaaagctttg
    10921 ttccgcataa tttagcggga gaaggaccgc gcggcggtgc gccggtggag atcgcctggc
    10981 cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca agctttgcag
    11041 attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat tctctgaaac
    11101 aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg aatacggcaa
    11161 cctggaaata atggaaaaga catataaccc acaagatatc gaacagccgc tttacgagca
    11221 ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaga gtttctgcat
    11281 catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct tccagcaaac
    11341 catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc tgtggcaggt
    11401 cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga
    11461 agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa tctgggaatg
    11521 gaaagcggag tccggcggca ccattacccg tcagatgcgc cgtctcggca actccgtcga
    11581 ctgggagcgt gaacgcttca cgatggacga aggcctgtcc aatgcggtga aagaagtttt
    11641 cgttcgtcta tataaagaag acctgattta ccgtggcaaa cgcctggtaa actgggaccc
    11701 gaaactgcgc accgctatct ctgacctgga agtggaaaac cgcgaatcga aaggttcgat
    11761 gtggcacatc cgctatccgc tggctgacgg tgcgaaaacc gcagacggta aagattatct
    11821 ggtggtcgcg actacccgtc cggaaaccct gctgggcgat actggcgtag ccgttaaccc
    11881 ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc tggttaaccg
    11941 tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa
    12001 aatcactccg gcgcacgact ttaacgacta tgaagtgggt aaacgtcacg cgctgccgat
    12061 gatcaacatc ctgacctttg acggcgatat ccgtgaaagc gcccaggtgt tcgataccaa
    12121 aggtaacgaa tctgacgttt attccagcga aatcccggca gagttccaga aactcgagcg
    12181 ttttgctgca cgtaaagcgg tcgttgctgc ggttgacgca ctcggcctgc tggaagaaat
    12241 taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtagtta tcgaaccaat
    12301 gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcgg ttgaagcggt
    12361 tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact tctcctggat
    12421 gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc gtatcccggc
    12481 atggtacgac gaagcgggta acgtttatgt tggccgcaac gaagatgaag tgcgtaaaga
    12541 aaataacctc ggtgccgacg ttgctctacg tcaggacgaa gacgttctcg acacctggtt
    12601 ctcttctgcg ctgtggacct tctctaccct tggctggccg gaaaacaccg acgccctgcg
    12661 tcagttccac ccaaccagcg tgatggtatc cggcttcgac atcatcttct tctggattgc
    12721 ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc
    12781 gttccacacc gtttacatga ctggtctgat tcgtgatgac gaaggtcaga agatgtccaa
    12841 atccaagggt aacgttattg acccgctgga tatggttgac ggtatttcgc tgccggaact
    12901 gctggagaaa cgtaccggca atatgatgca gccgcagctg gcggacaaaa tccgtaagcg
    12961 caccgagaag cagttcccga acggtattga gccgcacggt actgacgcgc tgcgtttcac
    13021 cctggcggca ctggcttcta ccggtcgtga catcaactgg gatatgaagc gcctggaagg
    13081 ttaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga tgaacacaga
    13141 aggtcaggat tgcggcttta acggcggtga aatgacgctg tcgctggcgg atcgctggat
    13201 tctggcggag ttcaaccaga ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt
    13261 cgatatcgcc gcaggcattc tgtatgagtt cacctggaac cagttctgcg actggtatct
    13321 ggagctgacc aagccggtaa tgaacggtgg aacagacgcc gaactgcgcg gtactcgcca
    13381 tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca ttccgttcat
    13441 caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg acaccatcat
    13501 gctgcagccg ttcccgcagt atgatgcgtc tcaggttgat gaagcggcgc tggccgacac
    13561 cgagtggctg aaacaggcga tcgttgccgt acgtaacatc cgtgcagaaa tgaatatcgc
    13621 gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt
    13681 aaatgaaaac cgtggcttcc tgcaaaccct ggcgcgtctg gaaagcatca ccgtgctgcc
    13741 tgccgatgac aaaggtccgg tttccgttac gaagatcatc gacggcgcag agctgctgat
    13801 cccgatggct ggcctcatca acaaagaaga tgagctggcg cgtctggcga aagaagtggc
    13861 gaagatcgaa ggcgaaatca gccgtatcga gaacaagctg gcgaatgaag gctttgtcgc
    13921 ccgcgcaccg gaagcggtca tcgcgaaaga gcgtgagaag ctggaaggct atgcagaagc
    13981 gaaagctaag ctgattgaac agcaggctgt tatcgccgcg ctgtaatcaa aacacagtga
    14041 tgaaaacgaa ggccggagca tgctccggcc ttttttatct cttacagaaa tggtaaagaa
    14101 ggcataattc cccgtgaaat actggctaag aagcctttat ctggttgttc ttctttattg
    14161 attaaaggaa ggtcatccaa atcgcccagc aaatacgtat tttttgcctg ccagtcaatc
    14221 atccatattt tcagcagtgg ataagccaga ccgcccgtaa tcccggatat caccaccaac
    14281 gcacacatgc gataaagcat accgtggtat gttaaagttg agcgaaaacg aatacgccca
    14341 tcattcagtg acaggttgct cataaaatga tttcgcaacg acaccgttaa ataactggtg
    14401 ctaacagcaa tcccaaaata atagattaac tgcgcgatta tcattttacg ttgcatttcc
    14461 ataaattgct gtaaattctc aatatcatca tttgcataca cagaactatc atacgcattt
    14521 aatatttgat cgaagataat ataaccagca acagcgagaa aaggtaataa agctaaaaat
    14581 gcgaggatgg catattttat acaatacgca gtatctaatt tcaccttgaa acgatgtata
    14641 ccgaaactgg tattgctcca gagaaaactc attaccaggc tatataaagt accattaaaa
    14701 ataccaatgg aaacaatacc aactattgcc atcagaacca cagatataat aacactactt
    14761 gaactattgg catgtagcat ctttgtcgag ataaagaaaa cagtccccat cccaatggcc
    14821 attaaaatcg gcaagaaaaa ggttacccac cagaaccctt tcatagagca attaaaacta
    14881 aatcttacgc cgttgagact ggtcatcaag gcctgataac gcagtccttt tgctgccata
    14941 aatataagca aaaccaacag tgacaaagtc aaaacacagc caacaagcgg catatctgct
    15001 gacactgtca ttaagattgc gaaatagaaa aaaacaaaaa caagacaact aaaaaaaaca
    15061 ttcccaccag taattccata agaaaatcgt tgtccgttaa cttccatatt agcatagaga
    15121 taacgcctac atttcattaa tgcccatggt aaatagatac ctagcgttat aatcgttaac
    15181 aacacattca ccacacaaat aagaaaatat ctctcacctg taccatgaaa aacaaacgaa
    15241 tgggacggaa catccatttc attaataact tgagccataa aaattccctg aattaaaaaa
    15301 tccattgata taatttatag atacagacag taaaatgttc tgccacgtta caatactctt
    15361 atcagtaata ggctggcaaa aactttattg ctttataaga cgcttgcgca cgtaagaaga
    15421 gagtggtatt aaacaaatgt ataattactt tatttttatt gggttaaact atgaataaca
    15481 ttgcgccgca atcacctgta atgcgtcgcc tgacgttgca ggataatcct gctatcgccc
    15541 gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg
    15601 ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca gccgggtcat gcatattggg
    15661 tggttgagta cgagggtgaa gtggtcggcg gcggtgggat tgcgccatta gcaggtagtg
    15721 aatcggacat ttgcgaactg caaaagatgt attttcttcc ggctattcgc ggtaaagggc
    15781 tggcgaaaaa actggcctta atggcgatgg agcaggcacg agaaatgggc ttcaaacgct
    15841 gctatctgga aacgaccgct tttttaaagg aagccattgc gctttatgag catttgggct
    15901 ttgagcatat cgactatgcg cttggctgca cgggccatgt cgattgtgaa gtgcggatgc
    15961 tacgcgaact ctaaaaaaat gcccgctagt gtgtgctatc gggcatttta atcaattaat
    16021 tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt catcgtcgcc gtcttcgccg
    16081 ttgggatctt caaagtaagt gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc
    16141 gtcatcagtt gttcaacctg ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg
    16201 atgtcgcagc aaatcacgat atcaccatct tcaacttcca gctcttctgg atcggtcact
    16261 tcgtaaccga gtttaaacgc ttcaactgct gctttttcca gggtttccag atcgtctgcg
    16321 gaaagatggt gttcgatggt gtacagtgcg tccggatcgc tgccatcttc cagtaattct
    16381 tcaataatca aacgtgtttc ttcacgctgt tcttccagtt gttccgggtt tgccatggct
    16441 cattcctctt aatgtcctgc cgatactcat attgtcacac accactggca ttgcctccac
    16501 ctttgtgaca aagatttatg ctttagactt gcaaatgaat aatcatccat ataaattgaa
    16561 ttttaattca ttgaggcgtt agccacagga gggatctatg tccgggtttt atcataagca
    16621 tttcctgaaa ttactcgatt tcacgccagc tgaactcaac agcctgctgc agttagccgc
    16681 gaagctgaaa gccgataaga aaagcggcaa agaagaagcc aaactcactg gtaaaaacat
    16741 cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc tctttcgaag ttgccgcata
    16801 tgaccagggt gctcgcgtta cttatctcgg cccaagcggc agtcagattg gtcataaaga
    16861 gtcgattaaa gacactgccc gcgtgctggg tcgcatgtat gacggtattc agtatcgcgg
    16921 ctatggtcag gagattgtcg aaacactggc ggaatacgct ggcgtgccgg tatggaacgg
    16981 cctgaccaat gagtttcacc ccacgcagtt gctggcggat ctcctcacca tgcaggagca
    17041 tttacccggt aaagcattca acgaaatgac gctggtctat gcaggtgacg cacgtaacaa
    17101 catgggcaat tcgatgctcg aagctgcggc gcttaccggt ctggatttgc gtctggtcgc
    17161 gccacaggca tgctggccgg aagctgcgct ggttacggaa tgccgcgctc tggcacagca
    17221 aaatggcggg aatattacgc tgacggaaga tgtagctaaa ggagttgaag gtgctgactt
    17281 tatctatacc gatgtatggg tgtcgatggg tgaagcaaaa gagaaatggg ctgaacgcat
    17341 tgcattgctg cgtgattatc aagtgaacag caagatgatg cagttgaccg gtaacccgga
    17401 ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac caaacgacgc ttggcaagaa
    17461 aatggcggaa gagtttggcc tacatggcgg aatggaagtg actgatgagg tcttcgaatc
    17521 tgccgccagc attgtatttg atcaggcgga aaaccgcatg cataccatca aagcggtgat
    17581 ggtcgcgacg ctcagtaaat aattgata
//
LOCUS       DAZFRG010000093        17508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFRG010000093 DAZFRG010000000
VERSION     DAZFRG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.093"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..385
                     /locus_tag="ACHKUC_002424"
                     /pseudo
     CDS             1..385
                     /locus_tag="ACHKUC_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            537..1583
                     /locus_tag="ACHKUC_002425"
     CDS             537..1583
                     /locus_tag="ACHKUC_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO6618672.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1580..2371)
                     /gene="nei"
                     /locus_tag="ACHKUC_002426"
     CDS             complement(1580..2371)
                     /gene="nei"
                     /locus_tag="ACHKUC_002426"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO6618673.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQY
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2407..3141)
                     /gene="pxpA"
                     /locus_tag="ACHKUC_002427"
     CDS             complement(2407..3141)
                     /gene="pxpA"
                     /locus_tag="ACHKUC_002427"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO6618674.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(3131..4063)
                     /gene="pxpC"
                     /locus_tag="ACHKUC_002428"
     CDS             complement(3131..4063)
                     /gene="pxpC"
                     /locus_tag="ACHKUC_002428"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO6618675.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFESDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(4057..4713)
                     /gene="pxpB"
                     /locus_tag="ACHKUC_002429"
     CDS             complement(4057..4713)
                     /gene="pxpB"
                     /locus_tag="ACHKUC_002429"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO6618676.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4736..5479)
                     /gene="ybgI"
                     /locus_tag="ACHKUC_002430"
     CDS             complement(4736..5479)
                     /gene="ybgI"
                     /locus_tag="ACHKUC_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO6618677.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLEVDAVIVHHGYFWKGESPVIRGMKRRRLKTLLANDINLYGWHLPLDS
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GSELVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5750..7231
                     /gene="dtpD"
                     /locus_tag="ACHKUC_002431"
     CDS             5750..7231
                     /gene="dtpD"
                     /locus_tag="ACHKUC_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO6618678.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDDGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7273..8691)
                     /gene="phrB"
                     /locus_tag="ACHKUC_002432"
     CDS             complement(7273..8691)
                     /gene="phrB"
                     /locus_tag="ACHKUC_002432"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO6618679.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVETVKQVCAENRITHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8688..9197)
                     /locus_tag="ACHKUC_002433"
     CDS             complement(8688..9197)
                     /locus_tag="ACHKUC_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO6618680.1"
                     /translation="MNQQLFDDSTIIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHAQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(9298..9504)
                     /locus_tag="ACHKUC_002434"
     CDS             complement(9298..9504)
                     /locus_tag="ACHKUC_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO6618681.1"
                     /translation="MELYREYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            9817..9906
                     /gene="kdpF"
                     /locus_tag="ACHKUC_002435"
     CDS             9817..9906
                     /gene="kdpF"
                     /locus_tag="ACHKUC_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO6618682.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            9906..11579
                     /gene="kdpA"
                     /locus_tag="ACHKUC_002436"
     CDS             9906..11579
                     /gene="kdpA"
                     /locus_tag="ACHKUC_002436"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO6618683.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     NTLGNAWVDLLRITLWVLTPVALLIALFFIQQGALQNFLPYQAVTTIEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSYTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            11602..13650
                     /gene="kdpB"
                     /locus_tag="ACHKUC_002437"
     CDS             11602..13650
                     /gene="kdpB"
                     /locus_tag="ACHKUC_002437"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO6618684.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            13659..14231
                     /gene="kdpC"
                     /locus_tag="ACHKUC_002438"
     CDS             13659..14231
                     /gene="kdpC"
                     /locus_tag="ACHKUC_002438"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO6618685.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTSNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            14224..16908
                     /gene="kdpD"
                     /locus_tag="ACHKUC_002439"
     CDS             14224..16908
                     /gene="kdpD"
                     /locus_tag="ACHKUC_002439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO6618686.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDAPLARTLNNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            16905..>17508
                     /gene="kdpE"
                     /locus_tag="ACHKUC_002440"
     CDS             16905..>17508
                     /gene="kdpE"
                     /locus_tag="ACHKUC_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO6618687.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQ"
BASE COUNT         3811 a         4730 c         4714 g         4253 t
ORIGIN      
        1 ggaaacactg gtgattgatt tgggtaaatt taacttactg gatattcgcc gccacacaat
       61 gtcgaaaaca ttcagtatca agacaacaaa atcgcaaaat gaccagtgta ccgacggatt
      121 taaggtcagc tcctcgtttt ataccgaaaa aacgttggtt gaagaagata aagcattgtt
      181 gattggtaat ggtctgaagc tacgtatact ggatgaaaac gcctcgcctt atacattcaa
      241 taaatatgct taatacgccg atttcaccag tgatatgctg atctacgaaa aaacctatac
      301 ggctgaatta tcgtccacac ctggcactcc catcgcggtg ggtcccttcg atacagtggt
      361 actttttaag attaactata actgatttaa cacgacgccc ttttcagccg gaaagggcaa
      421 cgtattcaga ttattgctga ttttcccctt ttccttgatt accgttattt tttccagacc
      481 cagctttctt tatgctgtat cgatacacca aagataaaat aatgaggaag cgaagaatgc
      541 ctgtattgca gtggggaatg ttatgtgttc tgtcacttct cctttctatt ggcttcctcg
      601 ctttacatct cccggcggca ctcctgcttg gcccgatgat cgccgggatc atcttcagta
      661 tgcgcggagt cactctgcaa cttccccgct ccgcttttct cgctgcccaa gccattcttg
      721 gctgcatgat tgcacaaaac ctcactggtt cgattctcac cacgctggcc gtcaactggc
      781 cgatcgtgct tgcgatttta ctggtgacgc tgctttccag cgccatcgtg ggttggttat
      841 tggtgcgcta tagctcactg cctggaaata ccggggcctg gggttcttct cctggcggcg
      901 cggcggcaat ggtcgctatg gcgcaagatt acggcgcaga tattcgcctg gtggcgttta
      961 tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt tctggtcacc cgcatgatgc
     1021 tgggcgataa cgctgaagcg gttaaccagc agatcgtctg gttcccgcca gtgagcatta
     1081 atctcctgct tacgattttg ctcgcggtcg ttgccggtac ggcgggatgc ttgctacgtc
     1141 ttccttccgg cacgatgctc atcccgatgc tggcgggcgc agtgctccag tctggtcagc
     1201 tcatcacgat tgaactcccg gaatggctgc tggcgatggc atatatggca attggctggc
     1261 ggattggtct tggtttcgat aagcaaatat tactgcgggc attgcgccca ctgccacaaa
     1321 tcctgctgtc gatttttgct ctgctggcta tttgtgcggg tatggcgtgg gggctgaccc
     1381 ggtttatgca tattgatttt atgaccgcct acctcgccac cagccccggc gggcttgata
     1441 ccgtggcggt catcgccgca gggagcaatg ccgatatggc gctcatcatg gcgatgcaaa
     1501 ctctgcgcct gttcagtatt ttgctgacgg ggcctgccat cgcacggttt atttcaacct
     1561 atgcgccgaa gcgttcggcc tagtgctggc agcctgggca ccagtaaaac gggcgcgatg
     1621 acagcgtggt tttctcaatg atgccgccac agcgttcgca aggttcgcca tcacgatgaa
     1681 aaaccttaaa gcgaaacagc gccccatgat atttgttttc atccacctgc ccccgcgtag
     1741 cgtaagaaag tcgcggaata tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat
     1801 tgagatcttt cgctttatga tttccggtca acccaacctg ccagaggatc tccacccgca
     1861 aataattgcc aagtcctgcc agaaacgcct gatcgagcag taatccagca aactggcggt
     1921 tacgaaagcg cggcgacaat aatcgttcct ttaccacctc cggcgtcaga ttcggatcca
     1981 gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc ggagtcaaca
     2041 tctcaatatc cgaggcgcta taaagcagaa tggttttgtc agccgtttgc agttttaccc
     2101 gcagtactcg cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa
     2161 gctgattatg gctgtagagc gttaagtcgt tggaaaaatg agttaacaac gccttaccac
     2221 gcgtttccac atgggtaacg tattgaccga taagtcgtga ttgataagat tttaactgcg
     2281 ggaaggcaaa ccagacatca gttagtggtt tgcctttgat cgccgcctcc aggttatccg
     2341 ctgcacggcg gatctccggg ccttcaggca tggtgatatc cttttttcat tctttattaa
     2401 gcggggttat gctgcgacaa caatcccctt ttcagcaaat gtagctcgta atcggcgggc
     2461 gaacgccagt gcgtgctcgc catcgccatg taggcagacc gtttgcgccg tgaccattgc
     2521 ccattcgccg gtgatacttt tgactctgcc gtgttgcacc atctccaacg tttgcgccag
     2581 cgcctgttct tcgttttcaa tcaacgcgcc tggctggctt cgcggcacca gcgaaccgtc
     2641 tgcctgataa ccacgatcgg caaacacttc ctcgcgcgtt accaggccat atcgttcgcc
     2701 tgcacgaatc agctcgcttc ctgccagccc gacgagaatc aatgccggat cgcaagcgta
     2761 taccgctctg gcgatggcgt ctgccagttg tgcctctttc gccgcctggt tgtataacat
     2821 gccgtgcggt ttgacatgac acattacgcc gccttgcgca cgggtaatcg ctgccagcgc
     2881 gccaatttga tacagcgtct gggcgaaaac agtttctggc ggcagctgca tggcgctgcg
     2941 accaaaattt tccctgtcgg gaaaactcgg gtgcgcgcca atcgcgacac cgttttttat
     3001 tgcttcacgc acgcaagcct gcatggtttg cgcatcgcct gcatgaaatc cacaggcaat
     3061 attggcagag gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc
     3121 ggcgttcagg tcaattttca ttgtgcagcc gccatgctaa ttgttcgaaa taacgttgtt
     3181 gatcttgccg tgctttcagt gcctcttcca gtgaacactg gacaaaatga atcggctgac
     3241 cgagcggaat ttgcgccaga tggtacatat cagcctcaat gatacaggca atacgcgggt
     3301 aaccaccggt ggtttgcgca tcgttcatca acacaatggg ctgcccgtta tgcggcacct
     3361 gcaccacacc tggtaacaaa ccgtgagata acagttcgcg atcggtggtg cgttttaaaa
     3421 tttgcccctg taagcgatag cccatccggt tactttgcga gctaagctgc cagggcgaac
     3481 gccagaatgc atcctgcgag gcgcgatcga actcatgata ttccggcccc ggcaaggcgc
     3541 gaatgcggtt gccccacagc agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg
     3601 ctttgccaat cgggagtcgg tcaccatccc tcagtaaacg gccttccagc ccgccaatcc
     3661 ccaccttgag atcggtgctg catgatccca ttaccggcgg aacatcaata ccacccgcga
     3721 ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctc
     3781 tcattggcaa tcgccagccg gtccagacgg cattatcatc cagccgtgct tcacagcctg
     3841 cgccagtcag ggcaaaccaa ccatcgcttt caaattcaac cgttaactga ccgagcgtga
     3901 tctcaagtgc gggcgcgttg gcatcattgc ccaccagcag gttagcaata cgtagcgcag
     3961 gcatatccag tgcgccacag tggctgatac ccgactggcg aaaaccgtga cgaccgccat
     4021 cctgcacagt ggtatacatg cccgcacgaa taatcttcag catactccct ccttctgggg
     4081 tacaaagcgc acgctgtctc ccggacgtaa taagatgggt tcgtcacgct ccggatcaaa
     4141 caggctgagt gaggtatgac caatcaactg ccagccaccc ggcgttgcca gcggataaac
     4201 accggtctgc ggcccgccga tcccgacaga acctgccgga acgtgcaagc gcggttcagc
     4261 gcgccgtggc gtgtgtagtt gttccggcaa actcccgaga tacgggaagc ccggttgaaa
     4321 acctaaaaac cagaccacgt attccacgga ggagtgcaat tcaacaacct gtttttcgct
     4381 caatccacaa tgcgccgcga ccaccgccaa atccggtccg gctgcgccac cgtaaaccac
     4441 cggaatttca ataaagcgag actccggctc cagcgcctca ctctcctccc accagcgttg
     4501 caaacgctct atggcatcca gcgccagcga ctcaggatta cgcaaaatca ctgtgatatt
     4561 gttcatgcca ggaatggctt caaccacatt cggcatatcc accagacgct gtgccagtcg
     4621 ccagatccgt ttctggctgg ccagcgtcac cggcggttcc agttccagta ctaccgccgt
     4681 ttcacctatc agataacaac gcgctcgttg cactttcgat ccctctgatt attcgttatg
     4741 caggattagg aatatcaata aaggtcacat caagatcggt attttcattc agccactcgc
     4801 tcaatgcgcg aataccaccg cgttcagtgg catggtgacc cgcagcataa aaatgcaatc
     4861 cctgctcgcg ggctgaatga atggtctgtt cagaaacttc gccagtaata aaagcatcca
     4921 cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc tcccgtgcac caggcgacgc
     4981 gctgaaccag ctcagacccg gtatcgccac accataacgg cttacgtccc agacgcgctt
     5041 caatccagga ggccagttcc agtcccggca caggcatggt cagttcgccc cacggcacca
     5101 acggctcaat ttcgcccatg acggtgatgc caagtaatgc tgccagttgt gcgttattgc
     5161 ccagttcagg atgggaatcc agcggcagat gccagccata caggttgata tcgttagcca
     5221 gcaaggtttt taaacgacgg cgtttcatgc cgcgaatcac cggagattca cctttccaga
     5281 aataaccgtg atgcacgatg accgcgtcga cctccaggcg taccgcttca tcaagcagtg
     5341 cctggctggc ggtaacaccg gtaacgattt tttgcaccgt ctctttgcct tccacctgca
     5401 aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag tttttcgtta atcagttgtt
     5461 ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg atagctacac cgttaacata
     5521 gcgttacctc gcctgttcgt cgatagccaa tcttttgaca tattaagaaa caaacccgac
     5581 gccaaaagac gcaaattgtt tatatcatca cgcttcagct aatgtatatt tatgcaatca
     5641 gacagcatat tgcataaata atcagatctg tatcaaagtt ttaactttaa ggaaacacaa
     5701 gcacgccgcg caactattac gtgacgttgc agtcaattgt ggaattatta tgaataaaca
     5761 cgcatcacaa ccgcgcgcta tttactatgt cgttgcgctg caaatctggg aatattttag
     5821 cttttacggc atgcgtgccc tgctgatcct ctatctcacc aatcaactaa aatatagcga
     5881 taatcacgcc tacgagttat ttagcgccta ctgttcgctg gtatatgtca cgccaatcct
     5941 cggtggcttt ttggcggata aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt
     6001 gttgatggcg atcggtcatg tggtgctggg tgccagtgag atccatccgt cattcctcta
     6061 tctgtccctg gcgattatcg tctgcggcta tggtctgttt aaatccaacg tcagttgcct
     6121 gctcggcgag ctgtatgagc caaccgatcc acgtcgtgat gacggtttct cgctgatgta
     6181 tgcggcgggt aacgtggggt ctattatcgc acctatcgcc tgtggtttcg cccaggaaga
     6241 gtacagttgg gcgatgggct ttggcctggc ggcggtgggt atgatcgcgg gtctggtcat
     6301 tttcttatgt ggcaatcgtc atttcacaca tacccgcggc gttaacaaaa aggtactgcg
     6361 tgcgacaaac tttctcctgc ccaactgggg atggctactg gttctgctgg tggcaacgcc
     6421 tgcgctgatt accgtgttgt tctggaaaga gtggtcggta tacgccttga ttgtcgcgac
     6481 gatcattggc cttggggttc tggcaaaaat ttatcgcaaa gcagaaaacc aaaaacagcg
     6541 taaggagctg gggctgattg ttaccctcac cttcttcagc atgttgttct gggcattcgc
     6601 acaacagggg ggtagctcga ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt
     6661 tggttatacc gttccgactg ctatgttcca gtcgattaat gccttcgcag ttatgctgtg
     6721 cggtgtgttc ctggcgtggg tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat
     6781 ctggggaaaa tttgctcttg gccttggcct gatgagcgcc ggattctgca ttctgacctt
     6841 aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg ccactgatgg tgttaggcct
     6901 ggcggtgatg ggctttgcgg aactgtttat cgatccggtt gctatgtcgc agattacgcg
     6961 cattgaaatc cccggcgtga ccggcgtatt aaccggcatt tatatgctgc tttccggcgc
     7021 gattgcgaac tatctggcgg gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc
     7081 ttccggggcg atcaactact ccatcaatgc atatattgaa gtatttgatc aaattacctg
     7141 gggcgcactg gcgtgtgtag gactggtact gatgatttgg ctgtatcagg cgcttaagtt
     7201 cagaaaccgc gcgctggcgc tggagtctta ataagccagc accatgcctg ataaaacgct
     7261 cgctgcgttt tatcaggctc ctttcctcgc ttcttcgtac gctgccagcg ttcgcagcct
     7321 tgcctctttg tgatcgacta tcggttgcgg ataatccaac gtcacacctg ttttctctgc
     7381 ccacttccac ggctcatgca ccgctttccc cggtacatcg cgcagctccg gtagccactg
     7441 gcggataaat tcgccttcgc ggtcaaattt ctcgccctga gttgtcgggt tgaaaatacg
     7501 aaaatacggc gctgcatcag tacccgtaga agcagcccac tgccagccac cgttattagc
     7561 tgccaaatca ccatcaatca actgcgacat gaaatatcgc tcgccttcgc gccagtcgat
     7621 caacaaatct ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc
     7681 agtgctgtta agctgacgca tggcagcatc gacaatcggg tatcccgttt tgccctcctg
     7741 ccaggccttt aaatgggcgg gattgctttg ccactgtaca cgatccgtcc aggcaataaa
     7801 cggacgatgt ttacacagcg aaggataata cgtcatcaaa tggcggtaga actcgcgcca
     7861 tatcagctca ttaagccaga cgctccccgg tccgccttcc agcacctgcg gttgttccgc
     7921 caacaggcga tgtaaacact ggcgaggcga taacccgccc gttgccaggc tggcggacaa
     7981 acgactggtg ccttccactg ccggaaaatc tcgttgttgc tcatactctc cggcaccgtt
     8041 ctggcaaaac tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt
     8101 atcgaaagac tgacgaggat aattcagcgt aatggatggc gcgggctcta tcgatccgct
     8161 actacgaact tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcaacca
     8221 tgcgttttta aaaggcgtaa agactttgta catctcatga ttgccagtca tcaccgcccc
     8281 cggcggcagg atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgtcctctc
     8341 agcctgcaca tcccgcgccc gctcattcac ctcatactga tagttataaa acagatgggt
     8401 aatgcgattt tccgcgcaca cctgtttaac tgtttcgaca ctggcagcaa agtcatccac
     8461 ttcgcggaac aataaaggaa tgcctttttc cgccagcgcg ttttgcagcg cattaagttg
     8521 tgtattgata agttcagcct gacgcggcga cacattatgc gccgcccact ggcgtggtgt
     8581 agcgatatac aacgccagca cgcgcgcaga cgaattgcgg caggcggcag ccagcgcgag
     8641 attatcgtgc tgacgtaaat cctggcgaaa ccagaccaga tgggtagtca taaaactcct
     8701 gcatggcgca agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag
     8761 gcgtcaggat aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagcggttgc
     8821 tcgccacggc gataactgtc gataagcgcc gccagctgct ggcgttctgt ggaatcaata
     8881 tgtgggcgaa aatatccctg agcgtgcatc aaaacgttag tatgatcacg ccggttggca
     8941 gggtgagaaa gcaaaacaat tacgcgttgg cggtactggt tgtaatagtc atccagactt
     9001 tgccaccggt gaatatcagc cacaaacggg ccaagtttgc ggtactccgg ctgagaatgt
     9061 gccagaaaga cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttagtcct
     9121 cgatctttga gattgtgtaa ctcatgcaaa gcaaaaatgc ggataatggt gctgtcatca
     9181 aaaagttgtt gattcattac tgctcctgaa cggtttacag gagtaagtgt agacgccgaa
     9241 aaaagaaaac cgccagcgtt tccaccggcg gttttcttta aacgtcatgt ttggcaatca
     9301 gtaaaaatca ccggttgcct tttcggcctg atccatccat accggtttat cgctggtttt
     9361 cgaccagacg cggtgcaggt agctataaaa gcgggcgcgg tctttccaga aaagcatgaa
     9421 aggcagcgcc agaacgcccg ctgcaaccgc acaagtacgg cgtaaaaaga taagccatgc
     9481 aggatattct ctgtagagtt ccatactttt ctcccccttt tgtatctacc cggtgaatgg
     9541 caccggaaaa atgaatttgt ttatctgatg aaaatagtac cgccttttgt gtaattttac
     9601 tactcatccg accacttatt tttgcttatt gatggtttat ttacattcat accgtaatta
     9661 agttacacaa aggttaaatt aatactaaac attagttaaa tcatggcttt tgccattttt
     9721 atactttttt tacaccacgc ccgcagattt ttgcgaaatc tttgcagcca gaactctacc
     9781 cttccagtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc
     9841 gtgttgctgg tgtttttatt actgggttat ctggtttatg ccctgatcaa tgcggaggcg
     9901 ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc
     9961 tggcgcgtcc cttaggtagc gggctggcgc ggctgattaa tgatattcct cttcctggta
    10021 cgacgggcgt tgaacgcgta ctctttagcg cgcttggcgt ctctgaccgc gagatgaact
    10081 ggaagcagta tctctcagcc attcttggcc tgaatatact ggggctggcg gtgctgtttt
    10141 ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt
    10201 gggatctggc gctcaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata
    10261 gcggtgaaac cacgttgagc tatttcagcc agatggcggg cttaacggtg caaaactttc
    10321 tttctgccgc cagcgggatt gcggtgattt ttgcgcttat tcgcgcgttt acccgccaga
    10381 gcatgaacac gctgggtaat gcgtgggtag acctgttgcg gattacgtta tgggtgttaa
    10441 cccccgtagc attgctgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttt
    10501 tgccttatca ggctgtgacg accattgaag gagcgcaaca gctgttacca atggggccag
    10561 tagcttctca ggaagcgatc aaaatgctcg gaactaacgg cggtggcttt tttaatgcca
    10621 actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcaa atgctggcga
    10681 ttttcttgat cccaacagcg ctgtgctttg cctttggtga agtggcgggc gatcgtcgcc
    10741 aggggcgcat gttgctgtgg gcgatgtcgg tgatttttgt catctgcgta ggcgtagtga
    10801 tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcggac agcagcatca
    10861 atatggaagg taaagagagc cgcttcggcg tgctggtcag tagcctgttt gcggtcgtga
    10921 ctacggcggc ttcctgcggc gcggtgattg cgatgcatga ttcgtatacc gctcttggtg
    10981 gcatggtgcc gatgtggttg atgcaaattg gtgaagtggt attcggcggc gtcggttccg
    11041 gcctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg
    11101 gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa ctgactgcac
    11161 tggcgattct ggtcaccccg acgctggtgc tgatgggcgc ggcgctggca atgatgaccg
    11221 acgccggacg tagcgccatg ctcaaccctg gcccgcatgg ttttagcgaa gtactgtacg
    11281 ccgtgtcgtc cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc
    11341 cgttctggaa ctgtttactg gcgttgtgca tgtttgtcgg gcgcttcggg gtgattatcc
    11401 cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca
    11461 cattgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg
    11521 gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat
    11581 gatattgagt gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt
    11641 gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat
    11701 ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg
    11761 gcaagcggtg tgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg
    11821 gtcaccgtac tgttcgctaa tttcgccgag gcgttggcag aaggccgcag taaagcgcag
    11881 gccaacagtc tgaaaggggt gaaaaaaacc gcttttgctc gcaaactgcg cgaaccgaaa
    11941 tatggcgctg cggcggacaa agttcctgct gatcaactgc gtaaaggtga catcgtgctg
    12001 gtagaagctg gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc
    12061 gatgaaagcg ccatcaccgg ggaatcggct ccggtgatcc gtgaatccgg cggtgatttt
    12121 gcctccgtca ctggcggcac gcgtattctt tctgactggc tggtgattga gtgcagcgtt
    12181 aaccccggcg agacatttct ggatcggatg atcgcgatgg tggaaggtgc gcagcgacgt
    12241 aaaacgccaa acgagattgc cctgaccatt ctcctgattg ccctgactat cgtcttttta
    12301 ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgcagt cagcgtaacg
    12361 gtactggtgg cactgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg
    12421 attggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt
    12481 gcagttgaag cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcacc
    12541 ctcggtaacc gtcaggcgtc agagtttatc cccgcacaag gcgtggagga aaaagcgctg
    12601 gccgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggccg cagtattgtg
    12661 atcctcgcca agcagcgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc
    12721 tttgtacctt ttactgcaca aagccggatg agcgggatca acatcgacaa ccgcatgatc
    12781 cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg tcacttccct
    12841 gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gagccacgcc gctggtggtg
    12901 gtggaaggtt ctcgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt
    12961 aaagagcgct tcgcccagct gcgtaaaatg ggcattaaaa cggtgatgat taccggcgat
    13021 aaccgcctga ctgccgccgc gatagctgcg gaagcgggtg tcgatgattt tctcgccgaa
    13081 gcaacaccgg aggccaagct ggcattgatt cgtcagtatc aggcagaagg ccgtttagtg
    13141 gcgatgaccg gcgacggcac caacgatgct ccggcgctgg cgcaggcaga tgtcgcggtg
    13201 gcgatgaact ccggtactca ggcggcgaaa gaagcgggca atatggtgga tctcgactct
    13261 aacccgacca agttgatcga ggtggtacat attggcaaac agatgctgat gacccgtggc
    13321 tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat tattccggcg
    13381 gcgttcgcgg caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc
    13441 gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt tttgattccc
    13501 ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac
    13561 ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat
    13621 ttactgctaa ccgtttgcgg tctggtgtga ggtttaccat gcgtggatta cgtccggcat
    13681 tatcgacatt tctctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg
    13741 cactggggca atggtggttt ccctggcagg ccaacggttc attgattcgt gaaggtgata
    13801 cggtgcgcgg ttcggcatta atcgggcaaa attttaccag caacggctat tttcatggtc
    13861 gcccgtcggc aacagcagaa atgccctata atccacaggc ttctggcggg agcaacctgg
    13921 cggtcagtaa cccggagctg gataaacaga tagccgcacg cgttgctgca ttacgggctg
    13981 ctaacccgga tgccagcacg aacgttccgg ttgaactggt gacggcgtca gcaagcgggc
    14041 tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcgtg gcgaaagcgc
    14101 gtaatctcag cgttgaacag ctcacgcaac tgatcgcaaa atacagccaa caaccgctgg
    14161 tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca ctggataagc
    14221 ttgatgaata atgaaccctt acgtcccgac cccgatcgtc tgctggaaca agctgccgcg
    14281 ccgcatcggg ggaagctgaa agtattcttc ggtgcctgcg caggcgtcgg gaagacctgg
    14341 gcgatgctgg cggaagccca gcgactgcgg gcgcaagggc tggatattgt ggttggcgtg
    14401 gtagaaaccc acggtcggaa agataccgcc gcaatgctga aaggtctggc tgttctgccg
    14461 ttaaaacgcc aggcgtaccg tgggcggcac atcagcgagt ttgatctcga tgccgccctc
    14521 gcccgccgtc cggcactgat cttaatggac gaactggcgc acagtaatgc gccaggttct
    14581 cgtcatccta aacgctggca ggacatcgaa gagctgctgg aagcaggcat tgatgttttc
    14641 actaccgtca acgttcagca tctggaaagc ctgaacgatg tggtcagcgg cgtcaccgga
    14701 attcaggtac gggagaccgt gcccgatcct ttttttgatg ccgccgacga cgtagtactg
    14761 gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt ctatattgcc
    14821 gggcaagcgg agcgcgccat tgaacatttt tttcgcaaag gcaatctgat cgccctgcgc
    14881 gaactggcac tgcgccgtac tgccgatcgc gtagatgagc aaatgcgcgc ctggcggggg
    14941 catcctggcg aagagaaagt ctggcacaca cgcgacgcga tccttttatg catcgggcat
    15001 aacaccggca gtgagaaact ggtccgcgcg gcggcgcggt tggcctcgcg cctgggcagt
    15061 gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga gaaaaaacgt
    15121 cgggcaattc tcagcgcctt acgtctggcg caggagctgg gcgcggagac ggcaacactt
    15181 tctgatccag cggaagagaa agcggtagtg cgttatgccc gtgaacataa tctcggcaag
    15241 attattctcg gtcgcccggc ttcgcgccgc tggtggcgtc gggaaacgtt tgctgaccga
    15301 ctggcgcgta tcgcccccga tctcgatcag gtgctggtcg cacttgatgc ccccctcgcc
    15361 cgcacgctta ataacgcgcc ggatagccgc tcttttaaag acaagtggcg ggtacagatt
    15421 cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc catgcagtgg
    15481 ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tgcttggcgt cgtggtggtg
    15541 gcactatttt acggacgctg gccttcggtg gttgccaccg tcattaatgt ggtgagtttc
    15601 gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca atatctgctg
    15661 accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc tggcgtgcgt
    15721 tatcaggcgc gggtagcccg ttaccgcgaa caacgcacac ggcacttata tgaaatgtcc
    15781 aaagctctgg ctgtgggccg tagttcgcag gatatcgccg ccaccagcga acagtttatt
    15841 gcctccacgt ttcatgcccg tagtcaggtg ttgctgcctg atgacaacgg taaattgcag
    15901 cctttaacac atccgcaagg aatgacgccg tgggacgatg ccatcgcgca gtggagtttt
    15961 gataaaggcc tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc gtaccagatt
    16021 ttgccgctaa aaagcggcga gaaaacctac ggactggtgg tggtggaacc ggggaatctg
    16081 cgccagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct gttagtcgcc
    16141 aatgcccttg agcgactgac actgaccgcc agcgaagaac aggcgcggat ggcaagcgaa
    16201 cgtgaacaga tccgcaacgc cctgctggcg gcgctttcgc atgatttacg cacgccgctt
    16261 acggtgctgt tcggtcaggc agaaatctta acgctcgatc tggcaagcga aggatcgccc
    16321 cacgcccgcc aggccagcga gattcgtcag catgtgctga acacgacccg actggtgaat
    16381 aatctgctcg atatggcgcg aattcagtcc ggcggcttta atttgaagaa agagtggtta
    16441 acgctggaag aagtggtcgg cagcgcgctg caaatgctgg aaccgggttt atcgtcgccc
    16501 atcaatcttt ctctgccaga gccgctgacc ttaatccacg ttgacgggcc actctttgaa
    16561 cgggtgctga ttaacctgct ggagaacgcg gtgaaatatg cgggggcgca ggccgaaatt
    16621 ggtatcaatg cccacgttga gggcgaaaat ctacaactgg atgtctggga taacggcccc
    16681 ggtcttccac caggccagga gcagacgata tttgataagt ttgctcgcgg gaataaagag
    16741 tcggcggtac cgggtgtagg gcttgggttg gcaatttgtc gggcgatagt ggatgtacac
    16801 ggcggcacca ttaccgcatt caaccgaccg gaaggtggtg cctgttttcg tgttacactt
    16861 ccccagcaaa cagcccctga acttgaagaa tttcatgagg atatgtgaca aacgttctga
    16921 ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag ggcgacggta
    16981 tgcgcgtctt tgaggccgaa acgctgcaac gcggcttgct ggaagcggca acccgtaagc
    17041 cagatttgat tattctcgat ctcggcctgc ccgacggtga tgggattgaa tttatccgcg
    17101 acctgcgcca gtggagcgcg gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg
    17161 ataaaatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg tttggcattg
    17221 gcgaattgca ggcccgtctg cgcgtcgcat tacgccgcca ctccgccacc accacgcccg
    17281 atccgctggt gaaattttct gatgttaccg tcgatttagc cgcccgcgtt atccaccgcg
    17341 gcgaggaaga ggtgcatctc acaccgattg agttccgcct gctggccgtg ctgctcaaca
    17401 acgccggaaa agtgctcacc cagcgccagt tactcaatca ggtgtgggga ccaaacgcgg
    17461 tcgaacacag ccactatttg cgtatttata tggggcatct gcgacaaa
//
LOCUS       DAZFRG010000094        17494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFRG010000094 DAZFRG010000000
VERSION     DAZFRG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.094"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..325
                     /locus_tag="ACHKUC_002441"
     CDS             137..325
                     /locus_tag="ACHKUC_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6618688.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(595..804)
                     /locus_tag="ACHKUC_002442"
     CDS             complement(595..804)
                     /locus_tag="ACHKUC_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618689.1"
                     /translation="MSKKHRSADEIFAEQRRLLLQSIISRQQEKARAKQMPPNTEPAP
                     TRFALDDFLDKHPRVMKANKNRPPG"
     gene            complement(862..1041)
                     /locus_tag="ACHKUC_002443"
     CDS             complement(862..1041)
                     /locus_tag="ACHKUC_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6618690.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKESE"
     gene            1241..1444
                     /locus_tag="ACHKUC_002444"
     CDS             1241..1444
                     /locus_tag="ACHKUC_002444"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6618691.1"
                     /translation="MLNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGSHHA"
     gene            1437..1901
                     /locus_tag="ACHKUC_002445"
     CDS             1437..1901
                     /locus_tag="ACHKUC_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6618692.1"
                     /translation="MRKNRLQKIITGLYASPVAHMVTGFSSLNTSGGQPPTSDKRFFC
                     VRKPTHTRIMAGRNGGAFVLAGFQMTSLLTLLRLATMFSSVVARLLKIHLGAFHMAVF
                     ARPYFVWRFMQCLTDSIAIFTVTAATEREARAQLPHAHLIFVARIRQGGSHA"
     gene            1894..2121
                     /locus_tag="ACHKUC_002446"
     CDS             1894..2121
                     /locus_tag="ACHKUC_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618693.1"
                     /translation="MHKIPFDVLVHSENALIRAKEMDALLLKLIDVPESGDESDSMMF
                     SVVRTLLTPVINELNTVMAIHENNKAQHTGE"
     gene            2127..2426
                     /locus_tag="ACHKUC_002447"
     CDS             2127..2426
                     /locus_tag="ACHKUC_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6618694.1"
                     /translation="MKNKNSGVTASGPARPEFMNGDIYRDKYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            2423..4171
                     /locus_tag="ACHKUC_002448"
     CDS             2423..4171
                     /locus_tag="ACHKUC_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFO6618695.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQMLAGRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTNNGIEYTPPAPGENIRDNAPNFH
                     KWLEHAAGKDSRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTTVIRAVVLATNNNPMIFTERAGGVSRRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFADPEKARALLIEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSSRYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDDCDAFL"
     gene            4461..4718
                     /locus_tag="ACHKUC_002449"
     CDS             4461..4718
                     /locus_tag="ACHKUC_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618696.1"
                     /translation="MHTSGKFNNQKKSHNRAIDLTEHWLRVAIKIIDRNAGEGYAKAH
                     PELISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            4715..5125
                     /locus_tag="ACHKUC_002450"
     CDS             4715..5125
                     /locus_tag="ACHKUC_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6618697.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMASMAVPLPCNQADDGQ
                     ATMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGSRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            5136..5393
                     /locus_tag="ACHKUC_002451"
     CDS             5136..5393
                     /locus_tag="ACHKUC_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6618698.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMLLVETDKEREHITMRRL
                     ECCRKSTRPALGPDNFSDIKKAADRVYAEMGLDKLKGETCQ"
     gene            5384..5644
                     /locus_tag="ACHKUC_002452"
     CDS             5384..5644
                     /locus_tag="ACHKUC_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618699.1"
                     /translation="MPVTTEEIKRHRELFGLEHPAHTTTERYRQHLAAGAIFWVDHHG
                     FLRSTLSGEILAAGPEQIDALIAYLEKVKPRTVQSVPPEDVN"
     gene            5928..7097
                     /locus_tag="ACHKUC_002453"
     CDS             5928..7097
                     /locus_tag="ACHKUC_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6618700.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAVADEERSKPGKASQTTDPAELRNYIVTGDVRSLSTTTNGGKDG
                     GYTVIPELDREVMRLLSDESVMRMIATVKTAKSNEYQKLVSTGGATVGRGTEGNKRTE
                     TETPKVERVSIKLNPIYAYPKTTQEILDFSEVDVLGWLSSEIADTFATTEEDDFVNGD
                     GTGKPKGFLNYTRDTNNDKARAFGTIEKMVTTTDAITADELIDILYKLKAKYRKNAVW
                     VMNSNTAAALQKLKNQSGDYVWRDSLKEGAPDTLLGRPVYYLESMPDIGKGKTPLAVG
                     DFKRGYFIVDHETGIRTRPDNITEPGFYKVHTDKYIGGGVVDSRAIKVLEMKNGD"
     gene            7133..7708
                     /locus_tag="ACHKUC_002454"
     CDS             7133..7708
                     /locus_tag="ACHKUC_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6618701.1"
                     /translation="MGEKNMKKSMEIRSSEITTSGASTLTGYVVRWNNLSDLLWGEFY
                     EKFQRGAFSEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTST
                     GRDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSG
                     VDLARRSLYEQHPEKMPRADNRRWWTDLAGV"
     gene            7710..8924
                     /locus_tag="ACHKUC_002455"
     CDS             7710..8924
                     /locus_tag="ACHKUC_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6618702.1"
                     /translation="MWPFRRKQEHRSLTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MVRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYRITDAYSGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EIEVKDDKEGKS"
     gene            8921..9259
                     /locus_tag="ACHKUC_002456"
     CDS             8921..9259
                     /locus_tag="ACHKUC_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6618703.1"
                     /translation="MRAGSMRDRIVIMSAKLSRLPSGAMKSTWEDVATVWAEVKGISG
                     RERMTAGAETAQATVRVWIRFRGDVTASSRIRVLTGAYSGNELDIVGPPVPDGKRTRL
                     EILCKTGAPR"
     gene            9256..9552
                     /locus_tag="ACHKUC_002457"
     CDS             9256..9552
                     /locus_tag="ACHKUC_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6618704.1"
                     /translation="MTAELITLEEVKLHCRIDGNEEDQLISGYIAAALEVCQKHIGRR
                     FDDGLEFTPAIKIGCMMYVSQLYEYRATVGDADTKEIPLAVGMLWSVYRDVGVY"
     gene            9552..9991
                     /locus_tag="ACHKUC_002458"
                     /pseudo
     CDS             9552..9991
                     /locus_tag="ACHKUC_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            9975..10160
                     /locus_tag="ACHKUC_002459"
     CDS             9975..10160
                     /locus_tag="ACHKUC_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618705.1"
                     /translation="MAMMTEQEQNRLIRGLIRQHDAWKTQETGHKANRTGRTERTTAK
                     RLTDRDREVMECFRNRW"
     gene            10280..10636
                     /locus_tag="ACHKUC_002460"
     CDS             10280..10636
                     /locus_tag="ACHKUC_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO6618706.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            10620..12281
                     /locus_tag="ACHKUC_002461"
     CDS             10620..12281
                     /locus_tag="ACHKUC_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6618707.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCAHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALDMGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEMNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECTGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            12295..12576
                     /locus_tag="ACHKUC_002462"
     CDS             12295..12576
                     /locus_tag="ACHKUC_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618708.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRT"
     gene            complement(13091..13234)
                     /locus_tag="ACHKUC_002463"
                     /pseudo
     CDS             complement(13091..13234)
                     /locus_tag="ACHKUC_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            13646..14011
                     /gene="mdtJ"
                     /locus_tag="ACHKUC_002464"
     CDS             13646..14011
                     /gene="mdtJ"
                     /locus_tag="ACHKUC_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO6618709.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLTKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            13998..14327
                     /gene="mdtI"
                     /locus_tag="ACHKUC_002465"
     CDS             13998..14327
                     /gene="mdtI"
                     /locus_tag="ACHKUC_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO6618710.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(14366..15187)
                     /gene="ydgD"
                     /locus_tag="ACHKUC_002466"
     CDS             complement(14366..15187)
                     /gene="ydgD"
                     /locus_tag="ACHKUC_002466"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO6618711.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVAGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(15287..15370)
                     /locus_tag="ACHKUC_002467"
     CDS             complement(15287..15370)
                     /locus_tag="ACHKUC_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618712.1"
                     /translation="MVGRYSFEFILIILILCALIAARFYLS"
     gene            complement(15463..15771)
                     /gene="asr"
                     /locus_tag="ACHKUC_002468"
     CDS             complement(15463..15771)
                     /gene="asr"
                     /locus_tag="ACHKUC_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO6618713.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            16042..16146
                     /locus_tag="ACHKUC_002469"
     CDS             16042..16146
                     /locus_tag="ACHKUC_002469"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO6618714.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(16196..17449)
                     /gene="ynfM"
                     /locus_tag="ACHKUC_002470"
     CDS             complement(16196..17449)
                     /gene="ynfM"
                     /locus_tag="ACHKUC_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6618715.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
BASE COUNT         4596 a         4193 c         4740 g         3965 t
ORIGIN      
        1 tttatcctga ttattcctct ttctgtaaac catacggcct caacatttgc caactctgaa
       61 tcatcctgac acaatcagac acatcccaac gcaatagaac acgaaataac tctttaagaa
      121 acgaaagggg gcaatagtgt taagcactga tcggtttata cgtgaaaaag aatgcgaaaa
      181 actaaccggc cttagccgta cgtgtcgcta ccgcctggaa aaggccggac aattcccatc
      241 acgtcgtaaa cttggcggtc gttccgttgg ctggtcttta tccgaggttc tggcctggaa
      301 ggatagctgc aaggcagttc attaatcacg ctggcggcac gcagccgcca cacatcaatc
      361 atctgaacac agagctataa ccatgaagat tgaatatacg ccagaacgtg ggcggggatt
      421 cgttcgccct ggtgaaactg aaaaacaaca aaattggggt ttttcaggaa taaaaaaagc
      481 ggccccgaaa tggagccgcc tttctgaaca gataacccgc tgcgccgttt gtgtgtgtga
      541 tctcaaacat aagcacgggg atgatagccg ctatcaggct ggtgggcaat gtgatcagcc
      601 tggtgggcgg tttttgtttg ctttcattac cctggggtgt ttatcgagaa agtcatcaag
      661 cgcaaatctt gtaggcgctg gttctgtatt tggtggcatt tgtttagctc gcgccttctc
      721 ttgctggcgt gaaatgatac tttgcaaaag caatcttcgt tgttctgcaa aaatctcatc
      781 tgctgatcgg tgttttttgc tcatagtact gacctgtaaa gcaatgcgcc ggagtacctc
      841 acgccacggc gctgatagtg attattctga ttctttggcc ttgcgacgct ggcggtattc
      901 aacttcccgc tttaatgcag ctgtaacaaa ctgccctgta ctttcgccgg gcatttttac
      961 cgcctcaaca ttgttcataa cttcatgtgg aacccttgcc gcaactgttt gtgattttgc
     1021 gtttactgct tttgtcgcca tgttgagcac ccttcataaa aaacaaatgc agtatgcagg
     1081 aaaaaaaata agtgttcaac acttgacgtg tttaacacct cgatttaaat tggtgttcaa
     1141 caccttatta aggcaaggtg tagaaacgac gaaaccccgc actgtaggag cagtaacggg
     1201 gcttctaacc accaacgata gcgaaagtat cgaggtagct atgttaaatc ataccacaca
     1261 cccgcaaggg cgggattcgc acaacctgaa taaatacatc tggcgtttta tcgccctgag
     1321 cacggcacaa ccgcgcgtga ttaccattga ggccaccagc gaacaggaag cacgccagca
     1381 atccccggct ggctgcgtga tggtattcgc cgcccgtatt cgtcaggggt cacatcatgc
     1441 gtaaaaaccg cttgcaaaaa attatcacgg ggctgtatgc ttcccccgtc gcccacatgg
     1501 tgaccgggtt tagcagcctg aatacatctg gcggacagcc gcccacatcc gataagcggt
     1561 ttttttgtgt ccgtaaacct acccataccc gcattatggc ggggcgtaac gggggagcct
     1621 ttgtgctcgc tggtttccag atgaccagtc tgctaaccct gttacgtctc gccaccatgt
     1681 ttagcagcgt agtagcgaga ctccttaaaa ttcatctggg agcctttcac atggctgtat
     1741 tcgcacgccc ttattttgtc tggcgcttta tgcagtgcct gacagacagt attgcaatat
     1801 tcaccgttac cgctgccact gagcgcgaag cacgtgcaca gctgccgcac gcacatctta
     1861 tttttgtcgc ccgtattcgc caggggggga gtcatgcata aaataccctt tgatgttctt
     1921 gttcattctg aaaacgcatt aatccgcgca aaggaaatgg acgcattact acttaagtta
     1981 attgatgtgc cggaaagtgg cgatgaatct gattcaatga tgttttctgt cgtgcgtacg
     2041 ctattaacgc ctgttattaa tgaattaaat acagtgatgg caattcacga gaataataaa
     2101 gcgcagcaca ccggagaata aaaaacatga aaaataaaaa ttctggcgtt actgccagcg
     2161 gccccgctcg tcctgaattt atgaacggcg atatttaccg cgataaatat ggcggcatgg
     2221 taacgattaa aggcgtggca gaacggcgca tcacctaccg tcgtgaaggc tacgaatatg
     2281 attgcgtgat gcctgtttat cagtttcggc gtgatttttc tctggtacag gcagcgcccc
     2341 gcagtaaacc aaccagcagg gagaaagcac gcgccaatat tcaggaaata aaaaagatgc
     2401 ttaacgtatt caggggtaaa aaatgaaact ggcaccgaac gtaaaaaaac agccacgcgg
     2461 aataaaacac aaagacacag aggtaattat tttcgcgggt agtgatgcct ggtcgcacgc
     2521 gaaacaatgg caggagcagg atggccccgc atccggcgat aatgtgccgc ctgtgtggct
     2581 tgggccaaat cagcttgccg aacttgatgc actgaaaatt gttccggatg gaaaaaaacg
     2641 cgtaaggctg taccaggccg gagaactgga tttggtggag accaaaaaga ttggtcagaa
     2701 gctggcggcg gcagatattc aggacgcaaa tttttacccc gaaggaatgc acgtccagaa
     2761 gtgtgaaaac tggcggcgct atctgaatgc tgagcgtgaa aatattgccg cagggcttac
     2821 catgccggag cagaaaaata cgcaactggc acaaatggca gacagtgagc gcgcacagat
     2881 gcttgctggt cgatttgatg gcgtttgtgt gcatccggaa agtgaaatcg ttcacgtatg
     2941 gcgcggcggg gtatggtgtc cggtcagcac aatggaactt agccgcgaaa tggtggcgat
     3001 ctattcagag cacagggcca ctttcagcaa gcgcgtaatc aataacgccg tggaagcgtt
     3061 aaaagttatt gccgaaccaa tgggcgagcc gtccggcgat ttgctgccgt tcgccaatgg
     3121 tgcgcttgac ctgaaaacgg gggaattttc cccgcacacg ccggagaact ggatcaccac
     3181 gaacaacggc attgagtaca cgccaccagc accaggggag aacatccgcg ataacgcgcc
     3241 aaactttcat aaatggcttg agcacgcagc cggaaaagac tcgcgcaaga tgatgcgtat
     3301 atgtgccgcg ctgtacatga ttatggcgaa ccggtacgac tggcagatgt ttattgaggc
     3361 caccggagac ggcgggagcg gtaaaagtac attcacacac atcgccagcc ttctggcagg
     3421 gaaacagaac acggtgagcg cggagatgac atcgcttgat gatgctggtg ggcgtgcgca
     3481 ggttgtcggg agtcgtctta tcgtcctggc agaccagccg aaatatacag gcgaaggcac
     3541 gggcatcaag aaaatcacgg gcggcgaccc cgtggaaatc aacccgaaat atgaaaagcg
     3601 tttcactacg gtaatcaggg cggtggtgct ggcgaccaat aacaacccga tgatattcac
     3661 cgaacgggcc ggaggcgtgt cacgtcgtcg ggttattttc cgcttcgaca atatcgtaag
     3721 cgaggcagaa aaagacaggg agctaccgga aaagattgcg gctgaaatcc ctgtcattat
     3781 ccgccgcttg ctggcgaact ttgccgaccc tgaaaaggca cgggctttac tcattgaaca
     3841 gcgagacggt gatgaagcac tggcgataaa gcagcagacg gatccggtta ttgagttttg
     3901 ccagttcctt aactttctgg aagaagcacg cggcctgatg atgggcggcg gcggtgactc
     3961 ttccaggtac acgaccagaa acagccttta ccgcgtctat ctggcgttta tggcgtatgc
     4021 aggcaggagc aaaccgctaa acgtaaatga ctttggcaag gctatgaagc cagccgcgaa
     4081 agtttacgga catgaatata ttacgcggaa agttaaggga gtaacgcaga ctaacgcaat
     4141 aacaacagac gattgcgacg cgtttttata atttttggca attgctgtct accttgtcta
     4201 cctgattaca taaaagttgt tttaattcag tgtattaatt taggtagaca gctttttttt
     4261 actgtctaca agctgtctac cttgtctacc tgtttttgta gccagccgga gggatgagta
     4321 gacatcaggt agatagcgat tttttactgt ctacttccat taaacccgcg tcattactgg
     4381 cctgagcatc tatcaggtag acagagtaga caaggtgggg gtaggtccaa aactttttaa
     4441 acgagggggt aaaaataaaa atgcacacat caggaaaatt taacaatcag aagaaatcac
     4501 ataaccgcgc cattgacctt acagagcact ggctgagggt ggcgataaaa atcatcgacc
     4561 gcaacgcggg agaaggatac gcgaaagcac atcctgaact aataagcgca ttcatgacca
     4621 cagcggcggc aaactttgcc acgctgacag aacgggagat tgccgaagcg gaacaggtaa
     4681 caaccatcaa cgttaaaacc ggagaggtgg aatcatgaca gcacagatag ccgcttacgg
     4741 gcggctggtg gctgacccgc agttaaagac caccagcaag ggcacacaaa tggctatggc
     4801 aagcatggcg gtccccctgc cgtgcaatca ggcagatgac ggacaggcga cgatgtggtt
     4861 atccgtcctg gcgtttggca gacaggccga cgcactggca aaacaccaca aaggcgagct
     4921 ggtgagcgtg gcgggtaaca tgcaggtaag ccagtggaca ggccagaacg gcgaaacgcg
     4981 gcagggctgg caggttattg cagacagcgt aatcagtgcc agaacggcgc gacctggtgg
     5041 gagccgacgc aagaccacag gcacacaggg taatcagcca ccagcgggag gcgatgaccc
     5101 ttacggtgat gatattccgt tctgagaggg tgacgatggt acatgaccgc atagcggaag
     5161 aactcgaggc gaaaggcttt taccggagag cgtcggcgcg atggggtgaa gtcatgctac
     5221 tggtggagac agacaaggaa cgggagcaca tcacgatgcg acggctggaa tgttgcagaa
     5281 aatcgacaag gccagcactc gggccggaca atttcagcga tattaaaaaa gccgctgatc
     5341 gtgtttatgc tgaaatggga ttagataaat taaaggggga aacatgccag taaccacaga
     5401 ggaaattaaa cgacaccgcg aattattcgg ccttgaacat ccggcccaca cgacaacgga
     5461 gcgttatcgc cagcacctgg cggcgggggc gatattctgg gttgatcatc atggtttttt
     5521 acgcagcacg ctttccggtg aaattctggc ggctggtcct gaacagattg atgcgctcat
     5581 cgcatatctg gaaaaggtga agccccgcac agtgcaatca gtaccaccgg aagacgttaa
     5641 ttaatggccc agccctggtt taaccagggc tttttttgtc agcttttgta aattatttgt
     5701 gcgtggttgt tcgtggttgt tcagcggcat ggctggctta ttttatcagg cccgaaccgt
     5761 ggttattctc gccactgttg ccaggacgct gggaccattt tcccgcctgt taatctcatc
     5821 agcgttaaca ggcgggaagc ctgcatcggt gtgcatttct ttttgttctc gtaccgtgag
     5881 tcttgccgcg tcctggtgtt ttcctctttg catcaggact tttttttatg ccgagaatta
     5941 tcgaattgcg ccagcagaaa accgccatca aaaatcagat gcgcgacatg ctggagaacg
     6001 cggaaaaaga aaaccgcagc cttaacgacg ccgagggcgc aaaatttgac gaattacgcg
     6061 ctaaagctga atccctcgat aaagacattt cccgccttga agccgttgcc gacgaagagc
     6121 gcagcaagcc aggtaaagcc agccagacca ctgaccccgc agaacttcgc aactacatcg
     6181 taacgggtga cgttcgctca ttatccacca ccaccaacgg cggcaaggac ggtggttata
     6241 ccgttatccc tgagcttgat cgcgaggtta tgcggttact gagtgatgaa agcgtcatgc
     6301 gaatgattgc gacggtgaaa acggcgaaat caaacgaata ccagaaactg gtttccacag
     6361 gtggcgcaac ggtggggcgt ggcactgagg gcaacaagcg aaccgaaaca gaaaccccga
     6421 aagttgagcg cgtaagcatc aaacttaacc cgatctatgc ctacccgaaa accacgcagg
     6481 aaatcctgga tttttcagaa gtggatgtgc tgggctggtt atcgtctgaa atcgccgaca
     6541 cgttcgccac gaccgaagaa gatgattttg taaatggtga cggtactggc aagccgaagg
     6601 gtttcctgaa ttacacccgt gataccaaca acgacaaagc ccgcgccttt ggcaccattg
     6661 aaaaaatggt tacaacaacc gacgccatta cagcggacga actgatcgac atcctctaca
     6721 agctgaaagc gaaataccgt aagaacgccg tgtgggtgat gaactcgaac actgcggcag
     6781 cattgcagaa gctgaaaaac cagagcggtg attacgtatg gcgtgacagc ctcaaagagg
     6841 gcgcacctga cacattgctg ggccgtcctg tttattacct ggaaagtatg ccggacatcg
     6901 gcaaaggcaa aacgccgctg gctgtcggtg actttaagcg cggttatttc atcgttgacc
     6961 atgaaacggg gatccgtacc cgaccggaca acattacaga gccgggcttc tacaaggtac
     7021 acacggataa atacatcggc ggcggggttg ttgactcccg cgcgataaaa gtcctcgaga
     7081 tgaaaaacgg cgattaacag aaaaaggggc taaaaagccc ctttggtgtt ttatgggtga
     7141 aaaaaatatg aaaaagagca tggaaatccg gtcatcagaa atcaccacca gcggagccag
     7201 tacgctgacg ggttacgttg ttcgctggaa taacctttct gatctgctat ggggtgagtt
     7261 ttacgaaaaa ttccagcggg gggcgttcag tgagtggctg gcggcgggta atgacgttcg
     7321 tggcctgtat gagcatgacc acagcatgtt actggggcgc acccgttccg gcacgctgaa
     7381 actggaagag gacgacacag ggttacgctt tgaactgacc ccaccggata ccagcacagg
     7441 gcgggacgtt atcgaactgg ttaaacgtgg tgatatatcc ggcatgagct ttggctttcg
     7501 ttcccgtaag gatgtatggg ataccacaac agatccatgc gtgcgcaccg tgctggtggc
     7561 ggaactgtac gaaattaccg ttacatccgt accggcttac cctgattccg gcgtggatct
     7621 ggcccgccgt tccctgtatg agcagcaccc cgaaaaaatg ccacgcgcgg ataatcgccg
     7681 ctggtggacg gatttagcgg gggtgtgata tgtggccttt cagacgaaaa caagagcatc
     7741 gcagccttac gctggatgaa tttatggcgc tggctggcac atcgaacacg ggggcgggtg
     7801 agtacgtatc atcggggaca gcggaatcac tccccgccgt catgaacgcc gtcacggtca
     7861 tctctgaggc ggtggctact atgccatgct acctgtacct ggtacgcaat gagaagggga
     7921 aggaggcccg cgagtggctt gattctcatc cggtcgatca tatcctcaac gagcgcccga
     7981 acgcgtggca gacgccctat cagtttaagc gaatgatggt ccgccactgc ctgttaaacg
     8041 gtaatgctta tgcggtgatt cagtgggggc gtgatggttt tccggtggct ttgcaccctt
     8101 acccgccgca gtcggtgaat gttgagcaga caggggaaca taactggcgt taccgcatca
     8161 ctgacgctta cagcggaaac acccgcaact atttaccgtg ggaggttctc caccttcgtt
     8221 actccacgga tgacggcttt atggggcgct cacctgtgac catctgccgc gaatcgctgg
     8281 ggcttgggct ggcccaacag cgccacggcg cgagcgtgat gcgtgatggc atgatggcag
     8341 caggggttat cacgtcaggc gaatggctgg acggcgtgaa gggcaaacag gcattagccg
     8401 cactggaacg ctacaaagga gccagaaacg ccggaaaaac gcccattctt gaagggggta
     8461 tgagctatca gcagcttggc atgagtaacc aggatgctga atggctggcc tcccgtcgct
     8521 tcaccattga agacatcgcc cgaatgttca acgtctcgcc gatttttctg caggaataca
     8581 gcaacagcac ctacagcaat ttcagcgagg caagccgcgc gtttctgacc atgacgatgc
     8641 gcccgtggct ggcgaacttt gagcagcaga taaaaaatgc tttgctggtg gcctcgcctg
     8701 tacctggtat ccggtatcag gtggagtttg acagcgcgga cctgttacgg gccacacctg
     8761 gcgaacgctt tgccacctat gaacgcggca tcaagtccgg cgttatgtgc ccgaacgaag
     8821 cccgcgaacg tgagggatta tcgccgcgtg atggtggcga tgagttcagc caggcttgga
     8881 aacaggagat cgaagtaaag gacgataaag agggcaaatc atgagagcgg ggagcatgag
     8941 ggatcgcatt gtcatcatga gcgcgaaact ttcccgcctc ccttccggtg caatgaaaag
     9001 cacatgggag gatgttgcca ccgtatgggc ggaggtgaaa gggatcagcg gacgggaacg
     9061 catgaccgca ggtgcagaaa cggcacaggc aaccgtaaga gtctggatcc gttttcgtgg
     9121 ggatgttacg gcatccagcc ggatacgggt actcacgggg gcttattccg gcaatgagct
     9181 tgatattgtt ggcccccccg ttcccgacgg taaaaggact cgtctggaaa ttctttgtaa
     9241 aacaggtgcg ccacgatgac agcagaatta atcaccctgg aagaagtgaa actccattgc
     9301 cgcattgatg gcaatgagga agaccagtta atcagcggat acatcgccgc cgcgcttgag
     9361 gtgtgccaga aacacatcgg caggcgcttt gatgacgggc tggagttcac gccagccata
     9421 aagattggct gcatgatgta cgtgtcgcaa ctgtacgaat acagggccac ggtcggggat
     9481 gcagacacaa aagaaatccc gctggctgta gggatgctct ggtcggttta tcgtgatgtg
     9541 ggggtgtact gatgccgtgg caaccattaa ggcgatgcac tgagccgggc tgtaataagc
     9601 gcgtgaagtc cggcaagtgt gaagagcaca ggcgggctgc atggcgtgca gaggatgcca
     9661 gacggggaca ccgccgcgca cgtgggtatt cccgacagtg ggacaaatac cgcgccatgt
     9721 acctgaaaaa aatccgttat gtgtgcgttg tctggctgag ggtatttata cgccagcctt
     9781 tgtggtggat cacattatcc ccatcaatgg tggcggtgat gttctcttct ggcctgagtg
     9841 gaatcaccag gcattgtgcc agacgtgcca caaccgtaag acgacgcggg aagatcccgc
     9901 cacgaaagca aaccgtaaag cgggcatgta tcgcgagcag gaagaacggg cggcacaccg
     9961 taacgactgg atgtatggcg atgatgactg aacaggaaca aaacaggctg attcgtggac
    10021 tgataaggca gcatgacgca tggaagacac aggagacagg gcacaaagcc aacaggacag
    10081 ggcgcacaga acgcaccaca gcgaagcgat taaccgaccg tgaccgcgag gtcatggaat
    10141 gttttcgcaa tcgctggtga ggccgtcaga gggggtgggg gtggttttca ggacgaaacc
    10201 gtccctgccg gacaccgacc gcctcctcaa atttttatgc acgggaattt tttgaaaaat
    10261 aatctgacga aaaataagca tggcaagacc accgaaagcc cccgcctacc tggatgacat
    10321 cgccgtgaag cagtggcggg aaaaatcgcg gcagcttgcg gaacggggag acctgacccc
    10381 cgccgactgg agcaatctgg aactgtattg tgtcaactac tccatttacc ggaaagccgt
    10441 cgcagacctt gcggcgcgcg ggttcagcat tgttaacagt cagggcggcg agagcagaaa
    10501 ccccgcatta agcgcaaaat ccgacgctga aagagtgatg ataaaaatgg cctccttgct
    10561 cggttttgac ccgataagcc gccgtaaaaa tccaccggaa acagaagaag aggacgagct
    10621 tgaccgcctg gaataagtac gcagaagacg taaaaacggg caaaattccg gcctgtaaac
    10681 ggctgaaaca ggccgttaaa cggtactttt cggaccttga aagccccctt tacacgttcg
    10741 atcgtgaggt tgtggagcgg tttattgcct tttccagggt gtgcgcgcac gtaaaagggc
    10801 cgatgcgtgg cagacccatt gagctggagc cgtggcagca gttcgccttt gcgtgcatcc
    10861 tcggctttaa ggttaaggcc accggacggc gcaaatacac gagcgccttt atcgaagtgc
    10921 cgcgcaaaaa tgcgaaatcc acgaccgccg cgattctggc taactggttt ttgattatgg
    10981 agaacggtca gcaggatatc tacaccgcag cggtgagccg tgaccaggcg cggatcgtct
    11041 ttgatgatgc gcgtcagatg tgccttttat cccgaccgtt acgcaggcgg gtgaatattc
    11101 aggcgcataa ggtgatacac ccgaaaacca acagcctgtt aaagccactg gcagcaaaag
    11161 cggcaaccat tgaggggaca aacccgagtc ttgccattgt ggatgaatat cacctgcacc
    11221 cagacaacgg ggtttattcc gcacttgata tggggatggg ggcgcgtccg gaggggctgt
    11281 tatttgccat caccacatcg gggagcaacg ttgtttcagc ctgtaaacaa cactatgact
    11341 attgctgtca gatactggat ggtgaagaga tgaacgaatc catgttcgtt ctgatttacg
    11401 agctggatga tgaaagcgag gttgacgatc cggcgatgtg gataaaggct aaccctaaca
    11461 tcgatgtttc cgtggatcgt gaaaaactgg cctcaaccat ccagaaagcg cggggtattc
    11521 cgtcgcagtg ggtggagatg atgacaaagc gatttaatat ctggtgtcag gggactaccc
    11581 cgtggatggg taatggtgca tgggctgagt gtaccggaac gttcacggag gaagatttac
    11641 acggtcagga gtgttacgcg gggctggact tatcatcaac cagcgatatt tccagcgtat
    11701 gctatgcctt tccggtcggt aaaaatatca tgctggtttc ccgtcattat ctgccggagt
    11761 tccagctaca gaacccagcc aataaaaatc gcgctgtcta tcgccagtgg gctaaagcgg
    11821 gctggatacg cacaacgcct ggcgactgca ttgattacga ccggatcaga gatgacatca
    11881 tgcaggacgc ggagaaattt aatatcaggc tggtgggctt tgatacgtgg aacgccacgc
    11941 atctcagaac gcaattacag ggggcaggtt ttgaggtgga gccgttcccg caaacctacc
    12001 tcagattcag tccggcggcg aaatcgttcg aagtttttgt taaccgcagg gtgattgtgc
    12061 atcgtggcga tccggtgttg tcctggtcga tgagtaacgt cgtgatgcag agtgacgcga
    12121 acgccaatat caagccgaac aagaaaaaat caccgaacaa gatagacccg agcgtggcgg
    12181 cgctgatggc gtttggtaca ttccaggcag agcatgagga ttttgctttt gatataagcg
    12241 acagccaccg ccagaaactg gaagaattta gcggggtgta atgaggtcag cagcatgaca
    12301 gaagccgaaa taacgggatt aatccgccgc gttgccggaa tcagccagca ggctgacgaa
    12361 caggccacgc agccggacag cgtgacagcc gaaaactatg cgcgtgttgt tgctgaggtg
    12421 atgcgccgtg atggtataga acttaatggc gtggatatgc gcaacatacg aaccagagtt
    12481 cttgagttgc tggcctaccg tcgccgcgtg gagatgtatc gggagaagga gaaaataacg
    12541 taccactgga agaagccgga acgactgcgt acgtaatacg tacggacatc gagataaaga
    12601 cggtttaacc atccattaaa aattgacgtg attttgtgct ttccggatga cgtggccaac
    12661 gtcatttttt agcgaaaaat tctgattaag gtcattggct gaatggtcat aatgaccacg
    12721 cagaccatca aggcaaccat aacgattttc gttacggttg atgtgtgcct tcgtccggca
    12781 ttcggacgat ataagggatg gtcataatga ccactcctac agaacaggta aaacccacca
    12841 gcctgattaa cgtttaaccg gaaaaaaatc caggtaggtg gagatcccca tatcgacagc
    12901 aacgcccctc atgaattggt gcgcttcccc ctggaaagat gatctgcctg tatatttctt
    12961 gtgtctattt gttccacgtt gtttcataca gtgcaccgaa cggtgtagtt actggtgtag
    13021 tcattttgcg atttttggca ctttttgtgg tgcaaattat ttaattaaat caatgtgtta
    13081 agtgaaataa gttatatata acagcaataa ataatgcgag aataaacggc acgatgatct
    13141 cggcggcaaa acggataccg cagagaataa tgaccagcat tcccaacata atgacgattt
    13201 ttaggccatt gagcgtgatg atcggctttg ccatgcttgt ccccgttttt tcttcctttg
    13261 ctaataataa tgtctttttc gctccgtcat cataacgaaa atcaaaccgc tcaggtaaag
    13321 aagtgaaaat attttgagtt aattcttaag ctatgataca aatcaggcgt gttcaactac
    13381 cgaggacaat tatcatccgc gaggacgaga agcaacactg cggataattg taatattatg
    13441 gacaatttgt tcaggacgtt attttctaca tttacccatc ttcgtacctc atccactatc
    13501 ttgctcgtag gtgagcagca ctggcgcaac gcgctttagt cctttcttct gccaaagctt
    13561 gcgctaagcg cggggcttga ccactttact ttggtttcgc tgaattaagc gaaaattaaa
    13621 ataattctct tgcaggagaa ggacaatgta tatttattgg attttattag gtctggctat
    13681 tgctacagaa attaccggta cgctgtccat gaaatgggcg agcgtcagtg agggaaatgg
    13741 cggctttatt ttaatgctgg tgatgatttc tctgtcgtat atatttctct ctttcgccgt
    13801 taaaaaaatc gccttaggcg tggcttatgc gctgtgggaa ggtattggta ttttatttat
    13861 taccttgttt agcgttttgt tattcgacga aagtttatcg ctgacgaaaa ttgccgggtt
    13921 aaccaccctg gtggccggga ttgtgttgat caagtcaggt acccgtaaag cgcgtaaacc
    13981 tgaactggag gtgaaccatg gcgcagtttg aatgggttca cgccgcctgg ctggcattgg
    14041 caatcgtgct ggaaatcgtt gctaacgtct ttttgaaatt ttctgacggc tttcgtcgca
    14101 aaatatttgg cttgctctct ctggcggcgg tgctggctgc ctttagtgcg ctttctcagg
    14161 ccgttaaagg gatcgacttg tctgtcgctt atgcattgtg gggcgggttt ggtattgccg
    14221 ccacgttagc cgcaggttgg atcttgtttg gtcaacggtt aaatcgtaaa ggctggattg
    14281 gcctggtgct gctgttggct ggaatgatca tggtgaaact tgcctgatga acacgctgcc
    14341 cgcgctgtcg ggcagcgttt gaacattatt tttgcgacag ttgatccagc ttgtcgcgaa
    14401 aaccggtaac agaaatggcc cggttatcgg cgcgccagcg atctttcgcg gcaggagccg
    14461 agctttgcac cccaattaat tgccagccgt catcggtatg caacatcaga ggcgaaccgc
    14521 tgtcacccgg caaggtatcg cactgatgcg acatcaccga cgtttgcgcc cagccagcca
    14581 cttcacagtt ttgatgactg tacaacgtat cgagatgatc ttcagggtag cccgcctgag
    14641 tcactttacg accagccgct tttaatgcgg cggtaagcgc ggctttatct ccctcaaata
    14701 acggcaacgg tgtaatgcca gaagggggat tacgtagcac aatcaaaccg aagtcccagg
    14761 gcgcagctgc gggaggtaca atccagccat ccccatctgc ttttaaccgc tttcccagtg
    14821 tcggatcaac gcggccttct atgtcgtgga tctcataacg ccaaagacct ttatttgaca
    14881 cgaaacgcag cgctaccgct ttatcggctt taccctttgg cggcgtcaat aaacagtgtc
    14941 ctgccgttaa tgccagattg ggtgcaatca gcgtcgccgt acataaattg ccgctggccg
    15001 tttccagttg gccaaccgca tcccacggtg attgggtcgt gtcattcact ggcacacgat
    15061 catcatgacc aaaaaacagg gtgctgacct catcgtttgc cgatttggca acgtctggtt
    15121 tatctgcaaa cacaaaagca gacgtcaaac taattgcacc caacactaca gcaatggttg
    15181 tacgcatatc acactctggt gggtaattat gattattaaa agcaaaccct cataaatact
    15241 atagacggga cgggatgaaa gtgggagtaa aatcagataa ctacgatcag gaaagataaa
    15301 aacgggcggc aatcagtgcg cataaaataa ggatgataag aatgaactca aagctataac
    15361 ggcccaccat actaccctcc gctaaaggcg gtgctaaacg ttacccgctt agcaccgtaa
    15421 ttttcaggcg cgagggggcg cgccagcatt actgttgaaa acttacgctg cgggttgtgc
    15481 agcaggtttt gccggttgct gatggctgtg tttcttggcg tgtttcttcg ccgcctgcgc
    15541 tttttgttca ggggctttct gttcagcttt ggcattttta tgatgctttt tagccgcctg
    15601 cgctttctgg gcaggtgctg ctttatgctg ttttttatga tgtgtggttt tcgcaggcgc
    15661 tgctttggtg gtcgtcgcag tcggagcagg tgtggtcgca gtctctgcag caaaggcagc
    15721 agaagacaga cccatagcag cggcaacaac cagagctaat acttttttca ttgtcatacc
    15781 ctcaatttgt tttttcattt aaccccactg cggggccgtt gaaataacta tatccctggg
    15841 aatggcagac ttccgtgagt ggttggtttc agcgtgtaac gatatgtaca aacgctgaat
    15901 agattacagc attgataata acgtattgtg acttaaggaa aatttagcta ccaataatag
    15961 tagtcttgat cgtgttaact attttactac ttacaaagcg gtagaataac tgcgcatcag
    16021 taataaatga cacacagcaa aatgaatccg tttatttggg tacttataat cctgatgacg
    16081 ctagatgcgc tgcgggaatt ggctggcgct tcgtctattt taggatggct attaacgttg
    16141 gtttgagcag gcaataagtc cggacgggga tttacctcag tccggactta ttttttcagg
    16201 cgtgcagacg atgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    16261 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    16321 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    16381 gttttgcgcg ggggccgatc cagctgctgg caacggagtg ggctgcgaag aatccggctg
    16441 agaagagtaa cattccggca aagatcagcc acagcgagct gaataaggtc atcagtaaac
    16501 caaacagcat aacccccgtc gaaaacaaca tcaccggacc gcgtccatag cgggtggtca
    16561 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    16621 ccacggcctg actgacatgc cagggggaaa gcatcaacct atagccgatg taattaaaca
    16681 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    16741 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagagaa gtcgggcgaa
    16801 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    16861 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacaccg ctaattaagc
    16921 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccattgaa aaggccacga
    16981 aactggggtg gatttcctcg ctaagataag tcatgccaac ggctgccacc ccacttaacg
    17041 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    17101 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    17161 catcggatag cgggccagta aacagcaaac cgatagccaa catcgccgtt gaaatggaca
    17221 gtgaaatact actgttcgcg ggtgttaagc caaactcctg cgaaagcacc ggaaggatag
    17281 gttgcacaca atagagaagt gcgaaggttg ccagtccggc agagaaaagc gccagggtga
    17341 cgcgcataaa ttgcggagta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    17401 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    17461 gctaaatatg cctgtggatc aggcttatga atag
//
LOCUS       DAZFRG010000095        17464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFRG010000095 DAZFRG010000000
VERSION     DAZFRG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.095"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..341)
                     /locus_tag="ACHKUC_002471"
     CDS             complement(<1..341)
                     /locus_tag="ACHKUC_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6618716.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPIAAGFKGLLPHGKKSRLQ
                     CDDDEGIMIDGCVARLRKYKPEEYELIIAHFVIGISLRAIAKKRKCSDGTIRKEMQTA
                     MGFIDGCLSFF"
     gene            complement(341..598)
                     /locus_tag="ACHKUC_002472"
     CDS             complement(341..598)
                     /locus_tag="ACHKUC_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618717.1"
                     /translation="MNNQTMTFTPEQLRKQAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            complement(595..1995)
                     /locus_tag="ACHKUC_002473"
     CDS             complement(595..1995)
                     /locus_tag="ACHKUC_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6618718.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILVTLPADVFS
                     VRAYRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMASLSTEKGIIQPVHIDDVL
                     PEVVERVECRNQGQEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVTHFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGE
                     ISKGLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMQQPQPR
                     RYSNKRTDSSKMERFF"
     gene            complement(1992..2891)
                     /locus_tag="ACHKUC_002474"
     CDS             complement(1992..2891)
                     /locus_tag="ACHKUC_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6618719.1"
                     /translation="MMTIDQREKQTRLQARMDDLRAEMDELRAEIAFAQKGEKPWPYR
                     SCLMREGRGYCEKHGKYRTHILVWIDRNGEDREKISCCPDCLIAEASDLTMELSSLKA
                     EELTDNAGIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSC
                     GTGKNHLAVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLD
                     LLIVDEVGVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRIT
                     DGGRNWLSFNWPSYRSRIGGVAA"
     gene            complement(2902..3828)
                     /locus_tag="ACHKUC_002475"
     CDS             complement(2902..3828)
                     /locus_tag="ACHKUC_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6618720.1"
                     /translation="MSSKILGNVWDACAAYGVKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLEQLARTERD
                     KVAELKQQRRLSVLRDPSDSELSKSEPSESVCSGVFEPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGIKPETMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDYWQQPA
                     AHGGGRQRQVDVLAGLGAMSDKFGKSSNKLTF"
     gene            complement(3825..4049)
                     /locus_tag="ACHKUC_002476"
     CDS             complement(3825..4049)
                     /locus_tag="ACHKUC_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618721.1"
                     /translation="MIRHIVNSLYHRYNRCPRVGQWFTTSNGLVLRVCLVNAESQKVV
                     CQVQGRTYTLSYPLVAFQSGKMFKRLGGVV"
     gene            complement(4046..4240)
                     /locus_tag="ACHKUC_002477"
     CDS             complement(4046..4240)
                     /locus_tag="ACHKUC_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618722.1"
                     /translation="MISYEIIISTTEYRNDVSVRTDVSVWHRRYKSRKTAELKAAEMC
                     ETISMKGSPVKYVTTAEVRP"
     gene            complement(4237..5088)
                     /locus_tag="ACHKUC_002478"
     CDS             complement(4237..5088)
                     /locus_tag="ACHKUC_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6618723.1"
                     /translation="MIRHQHIDSAISGRYTVSAPYKAGAGRGNPKFNIEHNRAHAVFS
                     CHEHRYAQIMVGRAGPTSVGPGSMLTGISTPVRLTTYMVVESLGGEFIEFNIGAVTMT
                     TLPTQSHPEITIINGRVVTTSLAVANYFTKRHERVLDRIRNLECSAEFTEHNFVLSEY
                     TDASGRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNLMEKSLSGADTSDMSA
                     VARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDSRLNG
                     SDKEVRP"
     gene            complement(5085..5747)
                     /locus_tag="ACHKUC_002479"
     CDS             complement(5085..5747)
                     /locus_tag="ACHKUC_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO6618724.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELHTAIYDAPASF
                     LSSALTHARIMVGWAGEPKGSPVSVCAGTANPVQSATSEFRSSGGGSYPLHTEAAIMA
                     TVPTSPFLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAETVQGHSAFRRNQREV
                     HL"
     gene            complement(5856..6563)
                     /locus_tag="ACHKUC_002480"
     CDS             complement(5856..6563)
                     /locus_tag="ACHKUC_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6618725.1"
                     /translation="MNTAIFNGKASMTSLEIAELVGSQHKDVKRSIERLMDKGIIRSA
                     PMANFEIINNLGLKRNVGAYIFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            6683..6976
                     /locus_tag="ACHKUC_002481"
     CDS             6683..6976
                     /locus_tag="ACHKUC_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618726.1"
                     /translation="MKVQAVGLFWFRDAIQYHELKNIFTDADVLSDSYTEWKHDAEKL
                     IKRVERGGQRVIKVEADTAEFIAWCTSEGIGINAEGRMQFASFKAYQQLLSER"
     gene            complement(7047..7256)
                     /locus_tag="ACHKUC_002482"
     CDS             complement(7047..7256)
                     /locus_tag="ACHKUC_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618727.1"
                     /translation="MTLYEILKIQFKTNAAIGRRFPKKGRPRGSQGVGKWKTRGVPED
                     VAILCHLDPSIPYTHPSLAHTEDGK"
     gene            7368..8024
                     /locus_tag="ACHKUC_002483"
     CDS             7368..8024
                     /locus_tag="ACHKUC_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6618728.1"
                     /translation="MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVAAALRCSPQWLQNGDEKDKHWENNVKSCPQRDTAHSYPVINWVQAGLFAT
                     SGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
                     GCYVIARKKSTNEATFKKYISDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
                     "
     gene            8128..8631
                     /locus_tag="ACHKUC_002484"
     CDS             8128..8631
                     /locus_tag="ACHKUC_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6618729.1"
                     /translation="MINKATTLDCLEELKNLGSLITLIAKATPDATLSSDIESCAGLA
                     WDMTNSISRKLSSAMLLQNKNSAINNRLRTQREACGLTTAELARLLDLDEEIIIQWES
                     GEYEPTISMLIPLANILGCDPMWLLTGEVTPPEQPKSEEQQHHDASQQVCPLSREALL
                     RKNQYQW"
     gene            8919..9125
                     /locus_tag="ACHKUC_002485"
     CDS             8919..9125
                     /locus_tag="ACHKUC_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618730.1"
                     /translation="MTETTSQQDNYEGICLEPDSFAVNVYHLLHATQVLHMSSNHETK
                     TLGSEILSFACEYTKAAAEKELAQ"
     gene            9320..9514
                     /locus_tag="ACHKUC_002486"
     CDS             9320..9514
                     /locus_tag="ACHKUC_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618731.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVIFIRHVHDDELVGTLSTFLFIA
                     EKAGYDVIAPADEDEDEDEE"
     gene            9520..10101
                     /locus_tag="ACHKUC_002487"
     CDS             9520..10101
                     /locus_tag="ACHKUC_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618732.1"
                     /translation="MQYDEFQAEATANGIRTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDILNEHGTTEHA
                     VVYNGMYGFMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDMGREGLEILLDEIINEMNTHGMQSETDIK"
     gene            10350..10640
                     /locus_tag="ACHKUC_002488"
     CDS             10350..10640
                     /locus_tag="ACHKUC_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO6618733.1"
                     /translation="MATDSQLTIETALNVGLALLGWLYIMSRTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQERAEKGESA"
     gene            10637..11371
                     /locus_tag="ACHKUC_002489"
     CDS             10637..11371
                     /locus_tag="ACHKUC_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6618734.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSIELDAAKQRIAELEGNFTALAAENAGIKSAIPESRDIEDDNMDDVSLAEDFGFNHA
                     IERMRRQIPETPTTDAFLAEVLAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMYPG
                     SDLVGKVEMTEWLVDFAAQLRKGGNQ"
     gene            11368..11853
                     /locus_tag="ACHKUC_002490"
     CDS             11368..11853
                     /locus_tag="ACHKUC_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6618735.1"
                     /translation="MSEINYQALREVAERAIPAMERLLMLPADDDLLSEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNLQYIKSRDQENEDIALTVGKLRVELEAEKQRA
                     KVLFMENARLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSIINSIATGIRIKG
                     E"
     gene            11855..12118
                     /locus_tag="ACHKUC_002491"
     CDS             11855..12118
                     /locus_tag="ACHKUC_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618736.1"
                     /translation="MATLTKKEQAWLSELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            12129..12641
                     /locus_tag="ACHKUC_002492"
     CDS             12129..12641
                     /locus_tag="ACHKUC_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6618737.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLA
                     AEPVAKIIAHYPLGVDVGKQKFVQAIRELPDFGGYLFAAPPAPIVPEEMYWQDAPVEG
                     SSKAAAYATGWSDCREAMLQSGNFRENKDSSTNNFRKIPEASTSSPVTPDGWISCSER
                     MPDDRQEVNQ"
     gene            12726..12968
                     /locus_tag="ACHKUC_002493"
     CDS             12726..12968
                     /locus_tag="ACHKUC_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6618738.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            12972..13118
                     /locus_tag="ACHKUC_002494"
     CDS             12972..13118
                     /locus_tag="ACHKUC_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618739.1"
                     /translation="MNKAFERWVHQRYGNRYDLTRDVDGFYCREVVKRMFEVWCHCRG
                     LSVV"
     gene            13127..13363
                     /locus_tag="ACHKUC_002495"
     CDS             13127..13363
                     /locus_tag="ACHKUC_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6618740.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRARKECWMVGREYI
                     HVSPDGNPKPSSECMYNRKAIDAWVASMKNKQPR"
     gene            13419..14732
                     /locus_tag="ACHKUC_002496"
     CDS             13419..14732
                     /locus_tag="ACHKUC_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6618741.1"
                     /translation="MDKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRTGSFDYAAQFPDSPNLQAFGVSKKEITVKELEEKWLDLKRMEISAN
                     AFNRYESVARTMVPKIGGSRLVSSVTKEELLYIRKDLLTGYQNSTKNKAAAKGRSVVT
                     VNYYMTTIAGMFQFAADHGYLEANPFQGIKPLKRARAEPDPLTRDEFIRLIDACRHQQ
                     TKNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVV
                     HLIQPAISVLKNQAEMTRLGKQYNIKVQLREYGRSVNHECTFVFNPQVVRKSEQVGFV
                     YKVDSVGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVF
                     NVYGAWMTDSNAEQIAMLNQKLTDYVPMMPHSHQSDIRGLLKSVS"
     gene            14714..15142
                     /locus_tag="ACHKUC_002497"
                     /pseudo
     CDS             14714..15142
                     /locus_tag="ACHKUC_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            15214..16737
                     /locus_tag="ACHKUC_002498"
     CDS             15214..16737
                     /locus_tag="ACHKUC_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6618742.1"
                     /translation="MKRVISYIRFSSERQAKGDSLRRQSKMVNDWLKAHPEYYLDQDL
                     SFRDLGVSGFTGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDVDQASELLR
                     NILRSGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRSHEESLRKSERIQAAWNRKK
                     ELISEGIKISKRCPAWLKLNDDRKSFSVIPEKVETVKRCFQLRLEGKSIWEIVRTLND
                     EGHQTLNQYKAGSFGQTSVQDLLKNRSVIGYFTPKGCEEIAGYYPAIIDETTFYKVQQ
                     LSYSRYGRKPASDKPMAVNLFKGVIRCSECGHALIITGFSDTRSGIYRCPLRNENRCS
                     AKGLSRKQTDGTLLTLLSRLDRFDSDMTNTIDTLKLHREHISSNIEKLVQLSLEIEDL
                     SAITIKLKQLKGELSQVDADIEKETQRLKSIDVGCLSDCDLTTKAGRIEAQLIIKSAV
                     KEVVLNTAKRRCKVSFHNGKVIDLSITENPSEDVTEAIQSLSEVTERGLIDVDEVII"
     gene            complement(16752..>17464)
                     /locus_tag="ACHKUC_002499"
     CDS             complement(16752..>17464)
                     /locus_tag="ACHKUC_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO6618743.1"
                     /translation="NVDAFLTLSATENMEFDSPMQVTTQNMQSGQTVTDNVQRAPRTI
                     TISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQKQIISVICRDGIKIDDSIITSF
                     KASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATGKTATTNDGGATSKKNTGNTTT
                     SLGNGMLMCQELYNRSTSELTNEQLQARINCGRSVKVENGKSTFSDAANEQASKVLSN
                     AGTGSTLKKFSVNPNKRY"
BASE COUNT         4820 a         4060 c         4088 g         4496 t
ORIGIN      
        1 atgaaaaatg acaagcagcc atcaataaac cccattgctg tttgcatctc tttccgtatt
       61 gttccatctg aacatttccg ttttttagca atagcgcgta atgagatacc aataacaaag
      121 tgagcaatga tcaactcata ctcttcgggt ttatactttc gcaaccgagc cacacaaccg
      181 tctatcatga taccttcgtc atcatcacac tggagacgtg actttttacc gtgtggtaaa
      241 agccccttga agcctgctgc tatcggctgc cagtccacac cactgttatc tgctgcagcc
      301 caggcccccc aacgatctaa aacttcgtac atatcacgca tcagcgcagt acctcctgca
      361 ccagtttttc aaacttacca actctggttt ccagctctgc cacacaatcc accagctcat
      421 ccactgcttt ttgtgcacgg tgttttgcct gcatcagttc cctgagcgct ggcaccatat
      481 cccgacgaat ggcatctttt gttacacctg ttttttccag ttgttccgcc tgccgcaaca
      541 tttcctgtgc ctgtttacgt aattgctcag gggtaaaagt cattgtctgg ttgttcaaaa
      601 gaaacgctcc atcttactgc tgtcagttcg tttgttgctg tatctgcgcg gctggggctg
      661 ttgcattggg gtggaaagaa cctgtgcgct ttcctgatcc acaggcagaa aatgtccgtt
      721 ataaaaacgc cgataaatcg tccccagaga accgttacgt tgtttcgtga tattgatttc
      781 tgcgatgccc ctggcctgcg tatccgggtt gtacacttca tccctgtaaa gcatcagaat
      841 gatgtctgca tccgcctcta tttctccgga atttttcagg tctgagttca tggggcgttt
      901 attgggtctg gactccacac cgcgggagag ctggctcagc gcaatcaacg gaaaaccacc
      961 ggattttgcc aggcctttaa gcccctttga gatttcaccc acggcaaggt catgacgccc
     1021 cgtggttcgg gtttttatca gcccgagata atcaaccacc accagcgccg tttccggatg
     1081 tttaatcagg tgatgtttcg ttgttgcgca tatctcatca atggtcaggt tcgcctggtc
     1141 caccatccag atattgcgcc cggtcatccg ccccactcct tgtgagaaac gtgcccagtc
     1201 ttcgtcttca aagtgagtga cagatttcag gcgtgatacc ggcattcctc cagccgcaga
     1261 taccatgcgt tcaccaatct ggatgttcgc catttccatt gtgaacagga gaacgccgtg
     1321 cccctgttca gtcaccttgt cgatgatatc cagcgccagt tcggttttac ccatcgaagg
     1381 acgagcagca ataaatacca ggtcgccggg ctccatgccg cctgtttttg cgtccagttc
     1441 atcaataccg gtcatcaacg ttctggattt ctcctgtccc tgattccggc attcaacacg
     1501 ctcaaccacc tccggaagca catcatcaat atgtaccggc tgaataatgc ccttttccgt
     1561 cgacaatgag gccatcatgt tctgcgcatc cttcagggca tcttcagctg cttcacaggt
     1621 atgcgcatca cgtaatttct gcaacgcctc attcagcgtt ttttctgcat cgcgcaatgc
     1681 ggcattgcgc cgcaacgctg caacatagtg ctccagtgaa gacttcaccc aggttttgcg
     1741 cccggtatca gtaatcaccg gggcaagttc cggcatctca ttacacaaca gcacagggtc
     1801 aatcacgcct gaaacacggg cctgtctgca gatgcctgtg tagatatccc tatacgctcg
     1861 tacagaaaaa acgtccgcag gtagtgtgac cagaatatcc atcacttcag gatctgcccc
     1921 acgcagaaag aacgcaccga tgacagcgcc ttccaggtca tcgttacgcc atgccggggt
     1981 gttctggctg gtcatgcggc aacacctccg atacgagaac ggtagctggg ccagttaaac
     2041 gacaaccagt tgcgcccgcc atcggtgatt ctgtcggcaa tccggggact gatgaacgcc
     2101 cacaattctt ccggtgaaag gttgctgatc aggatagttg gcaaaatacc ctcataccgg
     2161 gcattgataa tttcctgcaa aatggccatt tcagccgcac tgccaaactg aacgccgact
     2221 tcgtcgacaa tcagcaaatc cagtgacgca taatgctcaa tgacgtcatc cgctgttttt
     2281 tcactgtcat tccgccagca gtttttcaca gcccgggtaa ggcgcatcac gtcggtgatc
     2341 tccacactgg ccagatagtt acggatgatg tgttttgcca ttgataccgc cagatgattt
     2401 ttcccggtac cgcaactgcc ggtcataaca agactggtac cgttctccag catatctggc
     2461 cagttctccg catagcggcg acaggccgca agatttctgg ctgcgtcagg attaacctcc
     2521 agataattat caaactcgca gtcccgaaaa cgcagagcaa ttccggcgtt atcagtcagt
     2581 tcttccgcct tgagggacga cagttccatg gtcaaatcac tggcctcagc gatcaagcag
     2641 tcagggcagc atgaaatttt ttctctgtcc tcgccattac gatcgatcca caccagtata
     2701 tgcgtacgat atttaccgtg tttttcgcaa tatccgcgac cttcacgcat caggcaggaa
     2761 cgataaggcc atggcttttc gcccttctga gcaaatgcaa tctctgcccg taactcatcc
     2821 atttctgccc gtaaatcatc cattcgcgcc tgtagtcttg tttgtttctc acgttggtca
     2881 atcgtcatca tcgctgtcac ctcagaatgt caatttgtta ctggatttac cgaatttgtc
     2941 agacatggct cccaggccag ccaggacatc gacctgtcgc tgtcgcccac ctccgtgagc
     3001 ggctggctgt tgccagtaat cttcgaagtg acgatcgggt ccaaagaacg tcgcagcctg
     3061 cttcacgaac tgtgtgccgg tatttcctgt agcacgtacc caggcggcat accgcttcac
     3121 gccatcaagc atggtctccg gttttattcc ctccctgata cgggctttcc aggctttgaa
     3181 ggctgcagat tttgaattgc caccagcacg tttgggatat tcctgccagg cctgttcaaa
     3241 ttccggtgaa tattcctgtc tggcagaacg cgctggtgca gacgcgtcag cggatgcgcc
     3301 aatatcttgc ggttcatgtt ttgaatttac tggtggttca tgttttaaac cttgtggatc
     3361 tggagtcaga ttctgaaggg tcaaacgcgt atttttgcca gaatctgaag gctcaaacac
     3421 gcctgaacat acagattctg acggttcaga ttttgaaagt tcagaatctg aagggtcacg
     3481 taatactgaa agtctgcgct gctgtttcag ttctgcaacc ttatcccgct cagttctggc
     3541 aagctgctca agccgatcag cattcagatg ataaagattg gacgtattac ggttaccttt
     3601 gcggcgtgac tgacgcgtca gccagccatc agcctccagt tcggagattg ccgttctgac
     3661 tgtactttct cccaatccaa gctgtcgaca tatggtttca acacccgggt agcacacccc
     3721 gtcatcattc gaataatcag ccaggcgcgc cataatcatc agttttgcac ctttgacgcc
     3781 atatgctgcg catgcatccc agacattacc gaggatcttg ctactcatac gacacctccc
     3841 agacgcttaa acatttttcc ggactgaaac gccaccagcg ggtaactcag ggtataagta
     3901 cgcccctgca cctggcagac aaccttctga ctttctgcat tgaccaggca aacccgcaga
     3961 acgaggccgt tgctggtggt aaaccactgc cccactcggg ggcaacggtt gtatcggtga
     4021 tacagggaat taacgatgtg gcggatcatg gacgcacctc cgccgtagtt acgtatttaa
     4081 ccgggctacc tttcattgag atggtttcac acatctctgc cgctttcagt tccgctgttt
     4141 ttctggattt atagcgacgg tgccagacag atacatccgt gcgaactgat acatcgtttc
     4201 tgtattccgt agtggagatg atgatttcgt aactaatcat gggcgaacct ccttgtcaga
     4261 accattcagc ctggaatcaa caagtgcagc gccaaaaaca gcatcaccaa cacggtcgta
     4321 cagtttgcta gccagcggag attcaacggc cttaagcatt ggataaagct ggcttgtcca
     4381 gatttgatgg atttcacgca aatgcaggta tacgcctctg gcgtttcgtg cgacagctga
     4441 catatcagac gtatcggcac ctgataaact cttctccatc aggttaaagg cgttgatgta
     4501 tgcctctttg aaccgggcag cacgtttacc agtgaagccc atggcaagga acgcgaagcc
     4561 atcgcgggtg atttgatagc aaggtagttt gcggcctgat gcgtcggtgt attcacttaa
     4621 cacaaaattg tgttcagtaa attcagcgga acattcgagg tttctaattc tatctaaaac
     4681 ccgctcatgc cgtttagtaa agtaattagc tactgcaaga gatgtggtga caacgcgacc
     4741 attgataatc gtgatttcag ggtgagattg ggttgggaga gtagtcatgg tgacagcccc
     4801 tatgttgaat tcaatgaact caccaccaag gctttccacg accatatagg tggtgagacg
     4861 tacaggggtg gaaataccgg tcaacataga acccggccca accgaagttg gccctgcacg
     4921 ccccaccata atttgggcgt aacgatgctc atgacacgaa aaaaccgcat gagcgcggtt
     4981 gtgctctata ttgaatttcg ggtttccacg cccggcaccc gctttataag gtgcagagac
     5041 agtgtaacgt cccgaaattg cagaatcaat atgctggtgg cggatcatag gtgcacctcc
     5101 cgttgattac gacggaacgc ggagtgaccc tggacggttt cagcctcatg gaatgcttca
     5161 atacagctct cgtagtaccg cattgtgcgc agacttaacc caagctgaag catcatcaga
     5221 ccatcaaggg tgatgtaata accacgcagg gagtcaccgt agatgtgata agtacccggt
     5281 atgaaattgc gggtaaaaaa ctcgcgtgag cagttcagat actcgatttt gtcgacgatg
     5341 ttctggtgca tgcgcttaaa gtggcaggca acatgcaggg agaaaataac ggccttgccg
     5401 ttgacaactt caattttgag gaatggggaa gttgggactg tagccatgat ggcagcctcc
     5461 gtatgcaatg gataacttcc accaccggag ctgcgaaact cactggtggc agactgaaca
     5521 gggttcgcag taccggcgca tacggaaacc ggcgagcctt tcggctcccc tgcccagccc
     5581 accataattc tggcgtgcgt gagcgcggac gataaaaaag acgctggcgc gtcgtatatc
     5641 gccgtatgca attccgggct gcgacccccg gcacccgctt tataaggtgc ggagacagtg
     5701 taacgtcccg aaattgcaga atcaatattt ggtcttgaaa tgatcatata gctgctgata
     5761 tctttagaac tgttcttgga tgtttcggag ccgttttatg cgaaacagct ccccgttatt
     5821 gatgttgagt gagccgggtt actcccggct ttttttcatc gctgccaacc aataacctga
     5881 aataacccca ttttcgggtg ataccagcga gtccctcgcg gttctgcttc ctccataacc
     5941 cgataaaaag cagccataaa cggttccaca gcaacaattg cgcgacgtga caacaatccg
     6001 tccggcgtca tgaactcatg tgtgtctgta ggaatttgat aggcgttcac cagattgcgg
     6061 catttatcat ctgacaaacc ggtttttgct ttcagttggc gatatccggc atagccctca
     6121 cgaatagtgc cctttttaat ttgctcgact gtttcagcaa cgtggctgac tttttcttcc
     6181 acctgagtga tccgtttctg ttggcgaacg gcttcaagag ccatcgcggc aaccatttcg
     6241 atttcgctca ttggcttacg gatctgttct tccagttcgc gccagcgatc taccaggcga
     6301 gctgtgaatt caggacagag ctgtgcgacg acaatgatgc tgtcgcgctt accttgttcg
     6361 ccttcgaaga tgtaagcacc tacatttcgt tttaatccta agttgttgat tatttcgaaa
     6421 ttcgccattg gcgcacttcg aataatcccc ttatccatga gcctttcaat gctgcgctta
     6481 acatctttgt gttggctgcc caccaactct gcgatttcaa ggctggtcat ggatgctttg
     6541 ccgttaaaaa ttgcggtgtt cactgccatc tccttacgga taaattcttt taagattccg
     6601 cacattcgta cttattggtg ccgaaccttc cttcagttat ccttttgatc cctataaaca
     6661 aaagaaccaa aggaggttcg acatgaaagt tcaggccgtt ggtttattct ggtttcgcga
     6721 tgcgattcag tatcatgagc tcaaaaatat ttttactgat gctgatgtgc tctccgacag
     6781 ttacaccgag tggaaacacg acgctgaaaa attgattaag cgtgtcgaaa gaggcgggca
     6841 acgagttatt aaagttgaag cggatacagc cgagttcatc gcttggtgta caagcgaagg
     6901 cattggaatc aatgccgaag gtagaatgca gttcgcatcc tttaaggctt accaacaact
     6961 tctcagcgaa cgctaatgtg atcggggcaa tcgaaatggt tgtcccatcg tatttaatag
     7021 ttattttttc gctcatatca ccaccatcac ttcccatctt ccgtgtgcgc taggcttggg
     7081 tgtgtatatg gaatgctcgg atccaaatga caaagaatgg caacatcctc cggaacacct
     7141 cgcgttttcc actttccaac accttgactg ccacgaggcc ttcctttctt tgggaacctg
     7201 cgaccaatag cggcattggt tttaaattga atttttaata tttcataaag ggtcattctt
     7261 tagtctcaca ccagatactt tgttatccaa cgatgttaac cacaggaatc caaagtatca
     7321 agaaattctg ttactttagt atcaacagcc atgagaggag aagaaaaatg aagtctttag
     7381 gtgaacgtct catcaacgca cggcaaaaag ctgggttaac acaagatgcg ttggctaaaa
     7441 aagctgggat caccagagtt gcaatcagta aagccgagca aggccttaca aaaagtttca
     7501 acggtgacac cctttttaaa gttgcagctg cactgcggtg ttcaccgcag tggcttcaga
     7561 acggagatga aaaagataag cattgggaaa ataatgttaa gagctgccca cagagagaca
     7621 cagcacactc ttaccctgta attaactggg ttcaggcagg attattcgca acttctggtg
     7681 atgactacaa catgtatgat caggataatt ggaggcattc tgtaaaatac gctggtgaga
     7741 gggggttctg gctggaagtg cacggagact caatgacttc gcccgtagga ataacatttc
     7801 ctgaaggaat gtcgatcctt gtcaacccag ataaagaagt tttttcaggg tgttacgtca
     7861 tcgccagaaa aaaatctacc aatgaagcaa cattcaaaaa atatatttct gacatgggaa
     7921 aggcgtttct aaagcccctt aatccacaat atccaatcat agaaatggac aatgattgcg
     7981 aaatagtagg tgttgtggtt gatgccaggt gggatatttt ctgaccagac acaaaacaca
     8041 aaaagaaacc aaagtatcaa aaatcacttg ccacaccttg ataccttagt taccataaaa
     8101 caaagttcgt aactgaggta tcatctcatg atcaataaag ctacaactct tgactgtctc
     8161 gaagaactga aaaacctcgg cagcctcatt acactaatag caaaagcaac acctgatgct
     8221 acgctctcta gcgatataga gtcatgcgca ggactggcat gggatatgac aaatagcata
     8281 tccagaaagc tatcgtcagc aatgctttta cagaacaaaa attctgcaat caacaaccgt
     8341 cttcgcaccc aacgcgaagc ctgcggctta acaaccgccg aactcgccag gctgctcgat
     8401 ctcgatgaag aaattatcat ccagtgggag agcggagagt atgaaccaac tatcagtatg
     8461 cttatcccac tggcaaatat tcttggctgc gatccgatgt ggctgttaac tggtgaggtt
     8521 actcctccgg agcaaccaaa aagtgaggag cagcaacacc atgacgcatc tcaacaagtt
     8581 tgccccttat ctcgcgaagc tcttctgcgg aagaaccaat accaatggtg acataatcgc
     8641 cgcttcgccc ttcaaggtac atgcgaacat ttttatcaat cattgcggaa acagtctcaa
     8701 tatgaaaaca cttctgagac tcgctatata gcagaacata taagtcagct gaggaagcca
     8761 tgaaaaagtt cgaaaacata actgttctcc atgttgatga ctttgattat acaaacccgg
     8821 aacttctccc ggaggttgta aaggcaatag atgttgccga tatagtgatt agagaaaaga
     8881 gaattgtcaa aaacaggctc gcatgcactt caggagcaat gacagaaaca acctcacagc
     8941 aagataatta cgaaggcatt tgtctggagc ctgattcatt tgcggtaaat gtttatcatt
     9001 tattgcatgc aacacaggta ttacatatgt ccagtaatca cgaaacgaaa acactcggca
     9061 gcgaaattct gagttttgca tgtgagtata caaaagctgc tgccgaaaaa gaattagcgc
     9121 aataacaaca aatatgccct gaacgtttat tgcggtttta tcgccgggga ttattacaac
     9181 cttaatccac aggaggcttt attgtgactt ttataaagaa tatggcatca cacaagaccg
     9241 cctgccttat tgcacaatac ggtgaaaatt acatgcatat tgcctgctta tttctgcgta
     9301 aagcatacgg gagataataa tgcatcagaa aacagcagaa cacgaacaaa ccagagtatt
     9361 gctgaccatc aaaaacggga aagtaatatt cattcgccat gttcatgacg atgaacttgt
     9421 aggaactctt tcaacattcc tgtttattgc agaaaaggca ggatatgacg ttattgcacc
     9481 agcagatgaa gatgaagatg aagatgagga ataaatatca tgcaatacga tgaattccag
     9541 gctgaagcaa cagccaatgg tatacgaact ggcagtatga cgattgatta tcacgacgcc
     9601 atacgtcgtc tggatgccgg agaattcgat actcctaatg tgcgaggttt acgtatcctt
     9661 cagtgtctgg cgcaagccga cgaagcagga ttactgggta aacttccggt tgagatgaag
     9721 gttgctcagt ggcgatggtt gtatgtgacg acattcatca acgaagaaga agacaagaac
     9781 ggcacaattg atatcctgaa tgaacacgga acaactgaac acgccgtggt atataacggg
     9841 atgtatgggt ttatgacgat atatcccggc cccattcgat ttgccttaca acagtatatt
     9901 gaatggaatt taattcaaaa atacggcgaa gctgaaggaa tgggaagagc gctgtttctt
     9961 tatcagaaaa tgctcactac ttcccctgat aaaggtttca ttctttcaga tatgggtcga
    10021 gaagggcttg aaattcttct ggatgaaatt attaacgaaa tgaatactca tggcatgcaa
    10081 tccgaaacag atattaagta aaagggacca catgaccgtt atcgagtata tctaggaaaa
    10141 tccagattgc agtagagaag atatatccct cgcacttgga agaagcgcaa cttctatcag
    10201 taatgaatta tcacggttat tgtggaatgg gttaatagta cgaactggag aaaaaaacaa
    10261 aatgattctg tactgcgtaa acaatctgcc gtttggatac agcaatcccc taagtgttat
    10321 gttcaaccag ttacttaaac aggtaagaaa tggcaactga ctcacaacta accatagaaa
    10381 cggccctgaa tgtcggcctg gcacttctcg gttggcttta catcatgtcc cgtacatggc
    10441 gatggctggg ttccattttc ctaaaacagt ggaaaaaacg gcgcaaacag gaactacgcc
    10501 agaaggcatt agaagcgttc tatgacgcat ttgagcttag ccgcattgaa ccaggtacaa
    10561 cagccaggat agcgacaaaa ggcgacctga tgatagtgat gttccgacag gagagagcag
    10621 agaaagggga atcagcatga aattttccaa attttctgag ttggtgaatc gtattttgtc
    10681 caacaaccac agccatcgtc gcgatatgga tgtaacgatc gttgttcatt cgcctggcag
    10741 cattggttca acaccctcag ttgaagttca gtcaattcac gctggttttg attgggattc
    10801 cgggaaagtg ctgattttcc cagcacagcc actgaccact ctaacaccag aacagattac
    10861 tgatattact gatagtgtgc gcaaaggtca gtcctggcac gcatatcagg aatacaagaa
    10921 gcataaagag cagttggaaa aattatcgat tgaacttgat gccgcaaaac agcgcattgc
    10981 agagctggag ggtaatttca cggcgctggc agcggagaat gcgggaataa agtctgcaat
    11041 tccagaatca cgggatattg aagatgacaa tatggatgac gtatctctcg cggaagactt
    11101 cgggttcaat catgcaatag aacggatgag gagacagata cctgaaacgc caaccactga
    11161 tgctttcctg gctgaagtcc tggcgcaggg ggtggatgct gctatagaag ctgcaaaaaa
    11221 tctggtggcc caagaatatg agtataagga tttcaaagcg gcgcagagtg attgctgtat
    11281 gtaccctggt tcagacctgg tagggaaggt tgaaatgact gagtggttag ttgactttgc
    11341 tgcccagctt cgcaaaggag gcaaccagtg agcgaaatta attaccaggc actgcgtgag
    11401 gtggcggaac gtgcaattcc agcaatggaa cgcctgttaa tgttgccagc tgatgatgac
    11461 ttgttaagtg aacaggaact taaagattac ggtgtggata ttgatgcgct caacgccttc
    11521 aaatttctga ccggaccaga aaccgtgctg gcactgctgg atgaacggga aagaaacctg
    11581 caatacatca aaagccgcga tcaggagaac gaggatattg cgctaacggt agggaagctg
    11641 cgcgttgagc ttgaagcaga aaaacagcgg gcaaaagttc tatttatgga aaatgctcgg
    11701 cttaagtcag gcatagccgg tctgatacac ctcggtattc gatatgcaga tgttgaggtc
    11761 atgaaaattg ctggagatgc ccagctttct accccatgca ctgacagcat cataaacagc
    11821 attgcaacag gcattcgcat caaaggagag tgatatggca actttgacaa aaaaagaaca
    11881 agcatggttg agcgaattac aggacgttct tgatcgctgt ccatcaccga aaaaaattgg
    11941 tttttacacc attggcgata aaagcattta cctgtatgac ctgcgccgca tggatgaaat
    12001 catggaggct cttgataatc gttcgtcgat ggattggtgt gttgctgttc atgatatgaa
    12061 tgcagggttt gatgaaaaga ttttgttccc ctcatcagtt gaaagcactg cgggttaagg
    12121 agtaacacat gaccactata accaaagagc gactgctgac aatcaagcag tggcgcgaaa
    12181 catacggacc gggtagcaac gttgtgcttc cagcagaaga agcggaagag ctggcacgga
    12241 ttgcgctggc atcactggca gcagagccgg tggcaaagat tatagctcat tacccattag
    12301 gagttgacgt aggcaaacaa aaattcgtac aggccattag agagcttcct gactttggcg
    12361 gatatctatt tgccgcccct ccagcgccga tagtgccgga agaaatgtat tggcaggatg
    12421 cgccagttga aggcagcagc aaagcggctg catacgctac aggctggagc gattgccgcg
    12481 aagccatgct tcagtccgga aactttcggg aaaataaaga ttcgtcaacc aataattttc
    12541 ggaaaatccc ggaagcgtca accagctctc cggtaactcc ggatggttgg ataagctgta
    12601 gtgagcgaat gccggacgac aggcaggagg tgaatcaatg agctggcctg atgcaatcgt
    12661 aactctgggg gtggtattcg cagcagcgtt tgttgtgttc tcgatttgtc gatggggata
    12721 accacatgtt cgctttgatt caacgcggtc agatatacac ggacagagcc ggataccccg
    12781 tggtgattac tcgcatcact gagcactcag tgttctttcg acggatggac ggacgatccg
    12841 ggcgggtacg cattggtgag ttaaactgcc tgttcgaaca tattgaccac caggagtacc
    12901 gcaaaattct cgcggacact gagcaggaaa agcacctgaa aaaattacga gccataaaaa
    12961 ggaagtaaag aatgaataaa gcatttgaac gatgggtcca ccagcgttac ggcaatcgct
    13021 atgacctgac gcgagatgtt gacggcttct actgtcgtga agttgtgaag cgaatgtttg
    13081 aagtgtggtg ccactgccgt gggctgagtg ttgtgtgagg taatacatgg gcaatgtgat
    13141 tcaactggct cccaatgaat gggtttgtga aagcgttctt atcgcaatta ccgggctcaa
    13201 accaggcaca attcttcggg cccggaaaga atgctggatg gttggaagag agtatattca
    13261 cgtatcacca gacggtaatc caaagccttc cagtgaatgt atgtataaca gaaaagcaat
    13321 agatgcctgg gtcgcctcaa tgaaaaacaa acaacccagg tgatttaata tcatgaaata
    13381 tgtaagctcg tatcgctctt gggcgtctgg aggtatcgat ggataaagtc aaatatccaa
    13441 caggcgtcga aaaccacggc ggcacattac gcatctggtt taattttaaa ggtaaacgtg
    13501 tcagggaaaa tcttggtgtc cctgacactg ccaagaacag gaagatcgcc ggggaactgc
    13561 ggacatcagt atgttttgcc atccgcacag gaagctttga ttatgctgca cagttccctg
    13621 actcccccaa ccttcaggct tttggggtaa gtaaaaaaga aattacggtg aaggaacttg
    13681 aagaaaagtg gctggatctg aaacgaatgg aaatctctgc aaatgcattc aatcgctatg
    13741 aatccgttgc aagaacgatg gttccgaaaa ttggaggcag tagactggtg tcatcggtaa
    13801 ccaaagagga attgctgtat atcaggaaag atttgctgac cgggtatcag aattcaacga
    13861 aaaacaaagc agcagcaaaa ggacggagcg tcgttactgt aaattattac atgacgacaa
    13921 tcgctggaat gtttcagttt gctgcagatc acggttactt agaagcaaat cccttccagg
    13981 gaattaagcc tcttaaaaga gccagggcag agccagatcc gctaactcgt gacgaattta
    14041 ttcgcctgat agatgcttgc cgacatcagc agacgaaaaa cctgtggtca ttggctgtgt
    14101 acacaggaat gcgtcacggt gaactggtct ccctggcctg ggaagatatc gatctgaagg
    14161 caggaacaat taccatcagg cgcaattata cgaaacttgg tgagttcact ctaccgaaaa
    14221 ctgaagcaag cacaaacagg gttgtgcacc ttatccagcc cgctatcagt gtcctgaaaa
    14281 atcaggctga aatgacaaga ctgggtaagc agtacaacat caaggtgcaa ctacgtgaat
    14341 atggacgttc agtgaaccat gaatgtactt tcgtgtttaa ccctcaagtg gttagaaaaa
    14401 gcgaacaggt aggttttgtc tacaaagtcg attctgtagg tgactcatgg gaaacagcca
    14461 ttaagcgtgc agggatcagg catcgaaagg cataccagtc acgacacact tatgcgtgct
    14521 ggtcattatc tgccggagca aacccaagct tcattgccag ccagatgggc catgcaagtg
    14581 cccagatggt attcaatgta tacggagcat ggatgactga cagcaatgca gaacagatcg
    14641 caatgctgaa tcagaagctg acagattatg tcccaatgat gccccatagt caccaaagtg
    14701 acatcagagg cttattaaaa tcagtaagtt aatccttaat acccgtcatg ttaactgcgt
    14761 ggagggtaac accacgcttt atgccctgcc gaaacccgag gttgtcctgc gctggcgtga
    14821 gcagaccaca gatgacttcc gcttctgttt taagtttccg gcgaccattt cgcatcaggc
    14881 agcattacgg cattgcgatg atttagtgac tgaatttttg acccgcatgt caccgttggc
    14941 tccgcgcatt ggacaatact ggctgcaact gcctgccaca ttcggcccac gggagctgcc
    15001 tgcgctttgg cattttctcg attctcttcc cggtgaattt aattatgggg tggaagtccg
    15061 ccatccacag tttttcgcca aaggggaaga ggaacaaacg cttaatcgcg gtttacatca
    15121 gcgcggcgtt aatcgggtga ttttcatcag acacgtttaa atacaccacc taaagcgata
    15181 caatcgcgct attagttttt atggtggtaa agaatgaaac gtgtaatcag ctacatcaga
    15241 ttcagttctg agcgacaagc caaaggcgac tcactacgca gacagtctaa gatggttaat
    15301 gactggctta aggctcatcc tgagtactac cttgaccaag atttatcctt ccgtgatttg
    15361 ggggtgagtg ggttcactgg taagcacttg aaaggtggct taggtgactt tttaaccgct
    15421 atcgagaaag ggcttgttaa agctggtgat acgctcttga tagaatctct tgaccgtctg
    15481 tcacgtcaag atgtagacca agcaagtgag ctactcagaa acattcttcg ctctggtgta
    15541 gatgtggtca ccttgtccga tggtgaacat tacacacgag aatctcttaa agacccgtta
    15601 gctctgatta aaagcattct gataatgcag cggtcgcatg aggagagttt gaggaagtct
    15661 gagcgtatac aagctgcatg gaaccgtaaa aaagaactca tctctgaagg tatcaagatt
    15721 tctaaacgtt gtcctgcatg gcttaagctt aatgatgacc gtaaaagctt ctctgtaatc
    15781 cctgagaaag tggagactgt gaagaggtgc tttcagctac ggttagaggg taagtctatc
    15841 tgggagattg tcaggactct taatgatgaa ggtcaccaga cgcttaacca atataaagcg
    15901 ggttcatttg gtcaaacctc agtacaagac cttctgaaaa atcgctctgt aattggttac
    15961 ttcacaccta aaggctgtga ggagatagct ggttattatc ctgcaattat agatgaaaca
    16021 acattctaca aagttcaaca actatcatat tctcgttacg gtagaaaacc tgcttcagat
    16081 aagccaatgg ctgtgaatct tttcaaaggt gttatccgtt gttctgaatg tggtcatgct
    16141 ttgattatca caggctttag cgatacaaga agcgggattt atcgttgtcc ccttcgtaat
    16201 gaaaaccgtt gtagtgctaa aggactttca cgtaagcaaa ctgatggtac attacttacc
    16261 ctgctatctc gtcttgaccg atttgatagc gatatgacga ataccataga tacgctgaag
    16321 ctacaccgtg aacacatctc aagcaatatt gaaaagctgg tacagctatc acttgaaatt
    16381 gaagatttgt cagctattac catcaaacta aagcagttaa aaggtgagct atcacaggtt
    16441 gatgcggata tcgagaagga gacacaacgc cttaaatcta ttgatgttgg ttgtttgtct
    16501 gattgtgact tgactacaaa ggcgggaagg attgaagcac aactgataat caaaagtgct
    16561 gttaaagagg tcgttttaaa tactgctaaa cgtcgctgta aagtaagttt tcacaacggt
    16621 aaggtaattg acctatcaat aacagaaaac ccctcagagg acgttacaga ggctatacag
    16681 tcgctttctg aagtcactga gaggggttta atagatgttg atgaggttat catatagttg
    16741 agattctatc gtcagtatcg cttgttagga tttacagaaa atttcttcaa agtactacct
    16801 gtaccagcat tactaagcac tttcgaagct tgctcattag cggcatctga gaaggtgctt
    16861 tttccgttct caactttgac acttctacca caattgatac gggcttgaag ttgctcatta
    16921 gtgagttcac tcgtagagcg attatatagc tcctgacaca tcaacatacc attacctaga
    16981 cttgtcgtag tgttacccgt atttttctta gaagtagcac caccatcatt tgtggtagcg
    17041 gtctttccag ttgcagcaga gatatcagtc tgacccacaa tggctttgaa gttaatttcc
    17101 tgaaaagtta gctggattct tagaccattt gaaattccaa catctttaga ggctttaaaa
    17161 ctcgtaataa tggaatcatc aattttgata ccgtctcgac agatgacaga aataatctgc
    17221 ttctggtcac gccagctttc aagagtgtcg atgaaatttt ctactaattg accctgacga
    17281 gttaataata agcttccttc atagccaact acaacgacac cactaatagt gattgttctg
    17341 ggtgctcttt gcacgttatc tgttacggtt tgccctgatt gcatgttctg tgtagtcacc
    17401 tgcatagggc tgtcaaattc catgttttca gttgctgata aggttaagaa ggcatctaca
    17461 ttat
//
LOCUS       DAZFRG010000096        17332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFRG010000096 DAZFRG010000000
VERSION     DAZFRG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.096"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..533
                     /locus_tag="ACHKUC_002500"
     CDS             15..533
                     /locus_tag="ACHKUC_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6618744.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            549..1088
                     /locus_tag="ACHKUC_002501"
     CDS             549..1088
                     /locus_tag="ACHKUC_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO6618745.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1181..2758)
                     /gene="guaA"
                     /locus_tag="ACHKUC_002502"
     CDS             complement(1181..2758)
                     /gene="guaA"
                     /locus_tag="ACHKUC_002502"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO6618746.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2827..4293)
                     /gene="guaB"
                     /locus_tag="ACHKUC_002503"
     CDS             complement(2827..4293)
                     /gene="guaB"
                     /locus_tag="ACHKUC_002503"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO6618747.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4455..5831
                     /gene="xseA"
                     /locus_tag="ACHKUC_002504"
     CDS             4455..5831
                     /gene="xseA"
                     /locus_tag="ACHKUC_002504"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO6618748.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFELQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASLIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     AQQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5900..13951)
                     /locus_tag="ACHKUC_002505"
     CDS             complement(5900..13951)
                     /locus_tag="ACHKUC_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO6618749.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPTKTHEVAFTAKPSEFNADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPAVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLMLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEGGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNEQGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANAIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGDGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDIPFTLKRGDGYTRSEEKHVAGSSDALVAPVVVNGGLADETSLNNTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTARLYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGAVFKRPLLLKELSSTSGRTAIAEDNEDWAQFTQAQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADLTKFDRMNNVVKVKKGEEAVLRVTTKDAQ
                     GKPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQNDFLSTTLVIYGVTGAD
                     GTTTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQLTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGAINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMSVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGNVV
                     AMDDLVVQPLSGSAVTLSESGNTISGMTGADGTASFSLRQDNTPGYKMPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNSKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14072..15049)
                     /locus_tag="ACHKUC_002506"
     CDS             complement(14072..15049)
                     /locus_tag="ACHKUC_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO6618750.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELVTKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15104..17281)
                     /gene="sinH"
                     /locus_tag="ACHKUC_002507"
     CDS             complement(15104..17281)
                     /gene="sinH"
                     /locus_tag="ACHKUC_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO6618751.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINFATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRLNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDLLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDQAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMVQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4088 a         4600 c         4316 g         4328 t
ORIGIN      
        1 tataacaagg aatagtgatg aaatttaaaa aatgtcttct gcctgtggca atgttagcgt
       61 cattcactct ggcaggatgc cagtcaaatg ctgatgatca tgccgccgat gtttatcaaa
      121 ccgatcaact gaataccaaa caagaaacta aaaccgttaa tattatttcc attcttcccg
      181 caaaagttgc cgtagacaac gcccaaaata aacgaaatgc acaagccttc ggcgcgctta
      241 ttggtgcagt cgctggcggt gtaatcggcc acaacgtggg gtctggcagc aattccggaa
      301 cgacggcagg tgcagttggc ggcggagctg ttggcgcggc agcgggttct atggtgaatg
      361 ataaaacctt agtggaaggt gtttctctaa cgtataagga aggcaccaaa gtgtatacct
      421 ccacccaggt gggtaaagag tgccagttta cgacaggttt agccgttgtt attaccacga
      481 cgtataacga aacgcgtatt cagccaaata cgaaatgtcc tgaaaagagc taatgatcag
      541 gaggagtcat gaagaaagtt tttctttgcg ccatcttagc ctccttaagc tatccggcta
      601 tcgcctcatc attgcaggat caactctcgg ctgtcgcaga agcggaacag caaggtaaaa
      661 atgaagagca aaggcagcat gacgaatggg tcgcggagcg caacagggaa atccagcaag
      721 agaagcaacg tcgcgcaaac gcccaggccg cggctaataa aagagcggca acggcagcgg
      781 cgaataagaa agctcgtcag gataaactgg acgccgaagc cactgcggac aaaaaacgcg
      841 atcaaagtta tgaagatgag ctacgcagct tagagattca gaagcaaaaa ctggcgctgg
      901 cgaaagaaga agcccgcgtt aagcgagaaa acgaatttat cgatcaggaa ctgaagcaca
      961 aagctgcgca aaccgatgtg gtgcaatctg aagctgacgc aaacaggaat atgactgaag
     1021 gcggtcgcga tctgatgaaa agcgtgggca aagcagaaga gaataaatca gatagctggt
     1081 ttaattaatc gatgttagta acttcaatcc tatgattcgt gagtataaaa aaccctctgt
     1141 agtaacagag ggttttgttc attcatagtg cagagtcaaa tcattcccac tcaatagtcg
     1201 ctggtggctt gccgctgatg tcatacacca cgcgggaaat accattcact tcattgataa
     1261 tgcggttgga aacgcgaccg aggaaatcgt acggcagatg tgcccagtgt gcggtcataa
     1321 agtcgatggt ttcgacagca cgcagagaga caacccagtc atacttacga ccatcgccca
     1381 ttacgccaac ggaacgtact ggcaggaaga cggtgaacgc ctggctgact ttgtcgtaca
     1441 ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc acggcgcagc aggtcacaat
     1501 actctttctt cacttctccc agaacacgaa cgccaaggcc tggtcccggg aacgggtgac
     1561 ggtacaacat gtcatacggt aggcccagct ccagaccaat cttacgcact tcgtctttga
     1621 acagctcttt cagcggttca accaggccca tcttcatctc tttcggcagg ccacccacgt
     1681 tgtggtgaga tttgatgacg tgtgctttac cggttgcaga agcagcagat tcgataacgt
     1741 cagggtagat ggtgccctgc gccagccact tcacgtcttc cagtttcagc gcttcttcat
     1801 cgaatacttc aacgaaaacg cgcccgatga ttttacgttt tgcttccgga tcattttcgc
     1861 cagccagcgc tgacaagaag cgatcttctg ccggtacgtg aacaatattc agaccaaagt
     1921 gatcgccaaa catatccaga acctgctctg cttcgttgag acgcagcagg ccgttgtcga
     1981 cgaatacgca agtcaggttt ttaccgatag cgcggtgcaa cagcattgcg gttacggagg
     2041 aatccacgcc accagagagg ccgaggatga ctttatcgtc gcccacttgc tcgcggatgc
     2101 gggctacagc gtcgtcgata attttcgctg gcgtccacag ggcttcacac tggcagatat
     2161 cacgcacaaa acgctccagc atgcgcatac cctggcgggt gtgagtcact tccgggtgga
     2221 actgtacgcc atagaagcgt ttttcttcgt tggccatgat ggcaaacggg cagctttcgg
     2281 tgctggctac ggtgacgaag tcggacggga tagcggtaac tttatcgccg tggctcatcc
     2341 agacgtccag cagcggttta ccatctgcgg tcagcgcatc ttcgataccg cgaaccagtg
     2401 cgctgtcgtt tacgacttca acctgcgcgt agccaaattc acgttcgtta gaggcttcaa
     2461 cgtgaccgcc caactgcatt gccatggtct gcatgccata gcaaacgccg aataccggta
     2521 cgcctgcttc aaagacatac tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg
     2581 ggccgccgga aagaataatg ccgcttggat tgaagtcacg aatttgtgct tctgtcacat
     2641 cccacgccca cagttcgcag taaacaccca gctcacgcac gcggcgcgca accagttgag
     2701 tgtactgaga accgaagtcc agaatgagga tgcgatgctt atgaatgttt tccgtcattg
     2761 acgcttattc cgaggcaagt gaaacagata atataaatcg cccgacatga agtcgggcga
     2821 agagaatcag gagcccagac ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac
     2881 gtggctttcc tgaatacccg caccgctgat acgtacaaac tccgctttag tacgcagttc
     2941 gtcgatagta ccacagccgg tcaggcccat acaggagcgc aggccgccca tctgctggtg
     3001 aatgatctct ttcaggcgac ctttataggc tacgcggcct tcgatacctt ccggcaccag
     3061 tttgtcggca gcgttgtcgc tctggaagta acggtcagaa gaacctttgg acatcgcgcc
     3121 cagggaaccc ataccacggt aagatttgta agaacggccc tggtagagtt cgatttcacc
     3181 cggagattct tcagtacctg ccagcatgga acctaccatc accgcgcttg cgccagcggc
     3241 gatagcttta gcgatatcgc ctgagaaacg gataccgccg tcagcgataa ccggaatgcc
     3301 ggtaccttcc agggcttcta ctgcatcagc aacggcggtg atctgcggaa caccgacacc
     3361 agtcacgata cgcgtcgtac agatagaacc agggccgata cccactttaa cggcactgca
     3421 accggcttct gccagcgcgc gggcacctgc agcagttgcc acgttgccgc cgatgatttg
     3481 caggtcagga tatttagcgc gagtttcacg gatacgctgc agaacacctt cagagtgacc
     3541 gtgagaggag tcgatcagca gaacgtcaac gcctgcggca accagcgcat caacacgctc
     3601 ttcgttacca gcgcccgcgc caaccgctgc acctacacgc agacggcctt gttcgtcttt
     3661 acaggcgttc ggtttacgtt ccgctttctg gaagtccttc acagtgatca tgccgatcag
     3721 atggaattca tcatcaacca ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag
     3781 caccacttca cgggcttcgc cttcacgcac ggttaccaga cgctctttcg gcgtcatgta
     3841 aacactaacc ggctggctca agtcggtcac aaaacgcacg tcacgaccgg tgataatgcc
     3901 gaccagttcg ttttcttcgg taacaaccgg gtagccagca aagccgttac gctcggtcag
     3961 ttctttcact tcacgcagcg tcgtggttgg cagtacggtc tgcggatcgg ttaccacgcc
     4021 agattcgtgt tttttcacac ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt
     4081 gtggataaag ccgataccgc cttcctgagc cagagcaata gccaggcgcg cttccgttac
     4141 ggtatccatt gctgcggaaa gcatagggat attcagacga atagttttcg tcagctgagt
     4201 gctgaggtca gcagtattcg gcagaacggt agaatgagca ggaacgagga gaacgtcgtc
     4261 aaacgtcaga gcttctttag cgatacgtag catgggcaat atctcgacca gagtggttaa
     4321 taaatattgc cgcggcatta tacagagcgt aaccgattgc atctacccct ttttgcaaaa
     4381 aatgcttgct atccccggag ggcgggttac tatcgactga ataacctgct gatttagaat
     4441 ttgatctcgc tcacatgtta ccttctcaat cccctgcaat ttttaccgtt agccgcctga
     4501 atcaaacggt tcgtctgctg cttgagcatg agatggggca ggtgtggatc agcggcgaaa
     4561 tctctaattt tacacaacca gcctccggtc actggtactt tacgcttaaa gacgataccg
     4621 cgcaggtgcg ctgtgcgatg ttccgcaaca gcaaccgccg ggtcaccttc cgcccacagc
     4681 acgggcaaca ggttttagtt cgcgccaata ttacactcta cgagccgcgc ggcgattatc
     4741 aaattatcgt cgaaagtatg cagccggccg gtgaaggatt gctgcaacag aagtacgaac
     4801 agcttaaagc gaagttgcag gctgaaggtt tgttcgagct gcaatacaaa aaatcactcc
     4861 cctcccctgc gcattgcgtt ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata
     4921 ttttgcatgt gttaaaacgt cgcgatcctt ctctgccggt gatcatctac cccaccgccg
     4981 tacaggggga tgatgcgccg gggcaaatcg ttcgtgccat tgaactggcg aataagcgca
     5041 acgagtgtga cgtgttgatc gttggccgtg gcggcggttc gctggaagat ttgtggagtt
     5101 ttaacgacga acgcgtagcg cgggcgattt ttgccagcct cattccggta gtaagtgccg
     5161 tcgggcatga aaccgatgtg acgattgccg attttgttgc tgatttgcgt gcgccaacgc
     5221 cgtctgccgc cgctgaagta gtgagccgca atcaacagga gttactgcgt caggtgcagt
     5281 ctgcgcagca gcgcctcgaa atggcaatgg attattatct cgccaaccgc acgcgtcgtt
     5341 ttacgcagat ccatcatcgc ttacagcaac agcatccgca gctccggctg gcacgtcagc
     5401 aaactatgct cgaacgtctg caaaagcgga tgagctttgc gctggagaat cagcttaaac
     5461 gcgcgggtca gcaacagcag cgattaacac ggcaactcgt ccagcaaaat ccgcagtcgc
     5521 gtattcatcg cgcgcaaacg cgcattcagc aactggaata tcgtttagca gaaaccctgc
     5581 gcgcacagct tagcgccacg cgtgaacgtt tcggtaatgc agtaacacac ctcgaagccg
     5641 taagcccact gtcaacgctg gcgcgcggtt atagcgtcac cagcgccgct gatggcgcgg
     5701 tgttaaaaca ggttaagcaa gtgaaagtgg gtgagacact gaccacacga ctgggagatg
     5761 gtgtggtgat tagtgaagtc agcgccgtaa caaaaacccg caaatcacgt aaaaaaacgt
     5821 ctaatccgta gaccggataa gaggcgccag catcgcatcc ggcaataact cccaggctac
     5881 tccagatccg caatgctggt tactggaagc aggtcaccag acccggttta tttattagcg
     5941 tgatactaca atcctgtgct ccggtacctt cgtccatccc acactgttga gaggacaaat
     6001 aaggaaaact gggtgaagac ggccatccca gtgaattaaa aacagtatgc aacaactcta
     6061 attcattgta tgtcggtgca ttttctatgc tgccacactg ccttgcgata tcccatttac
     6121 tggcatcaat gatatgtgca gaagcccagt tttcgtagtt aataatcggt gaggatacgg
     6181 gattagcccc ggacggcaat tccgtcgtta acaacggtcg atgtagcgat ttactattca
     6241 ccactaccgt gtctgccata tgtccccaaa agtgcgccga tgaaacattt gggctggttg
     6301 gcaccgtaaa aatagcgtcc agcgtgtctg tcgcggaagc gtaattagcc agagtaaccg
     6361 tcagtggcat tttataacca ggggtattat cctggcggag cgaaaaactt gccgtcccat
     6421 ccgcgcctgt catgccacta atggtattac cgctttcact cagcgtaaca gccgaacctg
     6481 ataacggctg aacgacgaga tcgtccatcg ccactacatt gccatacaac gttgccccag
     6541 cgttacgcgg cgatgcctca ccacgtttca aagtaaattc aacattcggc accgtattcc
     6601 cggcgctgtc tttcaccgtc acggtaacgg acatggcttc gccttttttc actttcgccg
     6661 cctgagtggc tgcatcaaac gctgttgagg tcagtgtgac gctggaaacc gttgaacgcg
     6721 cgttagccag gcaaggctga aatgattttg ttgaacccgt atacagcgtg ccatttctca
     6781 aatcaaccga ctggtcttta ccatcggcgg atttatcctc tgcccaccag gtgtaatacg
     6841 caggccagcc agtttcagtg acaaacgtgt tattcggata acgcgcagac aacgaactta
     6901 aatcattgaa tagtggctgc cttgctactt cacaagggct cacactcgca tctgatttct
     6961 gtgttggggt aaagtacggc cagtcttcat tattgttatt gatcttgccg acgccagaag
     7021 tcgatgtcag ttcactccac aatttcgggc gttcaaatac cgcaccattc actgccgtca
     7081 aggttgctgg catatgcccc cagtagttag ccttcgcaga atccgggctg gtcactaccg
     7141 tgaatatcag gtcctgatta gccgtaacct gcggatttcg cgccaatgaa gctgtaaacg
     7201 gcgttaccag accaacggtg ttattttggg taacgttgaa cgttatcgtg ccatcactgc
     7261 cggtgaagcc gctccatgcc gacgtattct ggttgaagga gatacgcgcc gatgtcgggg
     7321 tgacttcatc taaaatgaga tcatctacgg tattggagga tacagagatc cctgaacggc
     7381 ttttcgcctg cacgcgcttg agcgtaacac cttcgccgcc aacaggatta ccattggcat
     7441 ctttgatcgt caccacgatg ggaagcatct cacctttttt cacagatgac gtgggggtac
     7501 caccattgct ggcagttcgg ccctcatcat aggcagtcga tgacagcgta aggcttgctg
     7561 gtgccgaatg tggctgcgtc agacagagca ttaaagcgta tccatttata gtcgtcgtcg
     7621 attgtcccgt tttcaaatta atggcgccgt aataccacgt caacgcgtca gtggtgctca
     7681 tttcataagt ccaccaattt ccacttccgg cagttatagg caaccccaaa tctgtcagta
     7741 ttgtcccgcc tggatgatca ttatatagcc cctgtaactc attgagacgc ggctgatact
     7801 caaccgggca atccggcgta actttattgg tataaaaaag gtatgaccac gactcattat
     7861 tattacttac cgaggacccc aaggtcgctt ctgctgccag tagcggtcgt ttaaaggtca
     7921 cccccgccct actggtaaac gtgtcgggca tatgacccca gtattttgct ttcggtgagt
     7981 tcggactggt tatcacagtg aaaataacat ccatcgtggc actttttgtg gtgtcgcgca
     8041 tcaatgttgc agtgagcgga gttgccagcc cataagtctc gttttgcgtc acatcaaaag
     8101 tcgctttacc atcacttcct gtcgtcagaa gtgcctgtgc cccattatta tagagtgaca
     8161 tgtttactcc ggcaggggtg accggcacga tagtaagatc atcatacgta gaatctagcc
     8221 tgttttttgc acgatccaac gcagtcccgc gtatgagatt aaagtttgca cctggcaaca
     8281 gatttcctgc gctatcccgc acaatcaccg tcatccggat cgccgtacct ttcgccgccg
     8341 ttgcactagg gcgaccatcg ttgctggccg ttttctccgc atccagtgcc gtggaggtca
     8401 tctctataga agctgccact gcatgagggt ttaccagaca ggacacaaat gccacggtgt
     8461 ttgataatgc cctggagctt gatccattcg ataaattaat agactgatcg taccagacgt
     8521 tcgcgtaggc aattttatcg taagcccacc aggtattagt cagcggaagg cctaaatcgg
     8581 tccctatcgc accattagga tgttcactgt ataactcttg aaattcgctt aataacggtt
     8641 gataaggttc gccgcagtct gatttgctga catcattttt tgctgccgta ttcaaagaaa
     8701 gccatgattc attattagcc atggttgact gccccgctgg atgctcggca gtaagcaatg
     8761 gccgcttaaa ggcaattccc gaccgggtag taaacgtttc aggcatatgc ccccagtaac
     8821 ttgccagtgg cgtatccggg ctggttatga cggtaaacat gaccggtaat ttgttggatt
     8881 ctatcgtcag ctgatacaac tgggcgtaca tattggttac caggcccata gaatcatctt
     8941 gttttaccgt aaaggaggta gtcccgtccg cgccagtcac cccataaagc aggcttgaag
     9001 aactattcat cctcgcagaa gcgcctgccg cgttaattac cgtaatcgac ttatttatca
     9061 tctcggcatt ttgacggttc ttcgagcctt cacgtctgag aataaagggg acattgggca
     9121 cgctatttcc ctgcgcattt ttagtcacca cgcgaatcac cgcgtcttcg ccttttttca
     9181 ctttcaacgc actcaaggtg tcgtcaactt tgacaaattg cgccgtatca ttcgcctcaa
     9241 caatgatctg actggcaacg gcgacaggcg tggtccggca gctcagtaat gcaccaccag
     9301 atgagggcat ctgcgtttca tctcctgtct tcagcgaaac gacatcatac gtaaaggttt
     9361 gagtgaacgt atctgcaacg gtattcgaga tatagaacct cgacactggc cagccaatag
     9421 cgtcatgtaa tgcgctactc ccctggtgcg cataaatggc cctaagttca ttgtcagttg
     9481 gaacatactc cgcgccacaa ccatttatcg aggtatcgtt agcctggccc gacgggaacg
     9541 tagaccaggc ctccccctct tccaccaggg taccgatact ggtggtagca ctattctcgt
     9601 ccctcagcaa aggccttcga taaataagac cgtcatcgcc tgtcgccgtt tcagccatat
     9661 gcccccagaa tttggctttt ggcgaatccg ggctggtaag caccgtaaag acaacgggaa
     9721 gcgtcgattt agtgctgctg gaggaatcca acgctgccgt gagttctgtt tttagcccgg
     9781 tagtctggtc ttgcttcaat gtgaaagtgg tggtgccatc agcccccgtc acgccataaa
     9841 taaccagggt cgttgaaaga aaatcatttt gagtatttcc ccaggcatcc gttaccgcca
     9901 gtgaagcaat cgacgtcgta gttgacacat tggccctgtt cacgctggtg tttcgtttga
     9961 gggtaaatgc ggtatttccc acaggcttac cctgcgcatc tttagtggtc actcgtaata
    10021 cggcttcttc accctttttc actttgacaa cattattcat acggtcaaat tttgtgaggt
    10081 ctgcagcttc aagaacgatt tggcttgcct taacaatcgg cgtcgtctgg caggtaagca
    10141 gttcttttgt ggcagatgga attatcgaac tggtgcctga actgagattg acggccttga
    10201 agtcacgctg ttccaggcta ctactacctg tcgtgctgga aaggtaactt gaggcgacgg
    10261 gccagccctg aaccgttttc atcgcatttc cgcggttggc ttcatacaat gactccagcc
    10321 cggcctgaga gggaacatat tcactaccac aaccattaga ggatgtgctt atagcctgag
    10381 cttgggtaaa ttgcgcccag tcttcattat cttccgcaat cgctgttcga cccgacgtac
    10441 tggataactc tttcaacaat aatgggcgtt taaagaccgc tccatctgca gccgtcactg
    10501 tttcaggcat atgcccccac attttcgcct tatcgctgtc cggactggtt acaacggtaa
    10561 aaatggtatc gagggtcgct tttttcgttg tatctgaata gagcctagcg gtcagcgacg
    10621 ttttcgtgcc atgggtatcc gggcgggtaa tagtgagaat tttagttccg tcgctgccgg
    10681 tcatggcgct gtatgctgca gcggtattat taagcgaagt ttcgtccgcc aggccaccat
    10741 tgaccacaac cggcgcaacc agcgcatcac tgctaccagc aacgtgtttt tcctctgagc
    10801 gggtataacc atcgccacgt ttcagggtaa agggtatatc acccaatgga ttgccctgcg
    10861 catcttttac cgtcaccttt acctgtagcg tttcgccttt tttcagcttc gccgcatttg
    10921 cagccgcgtt ccactgcgcc ggatccacca cttccagcgt aatgctactg gcaggattgt
    10981 tagccgtcgt caggcaactg acataaaccg ccgtgctacc gccaacggtc cgcgccccat
    11041 cgttgagggc tatggtgtaa taatgcggta cctgatctgc gggcgaactg ctccagtagg
    11101 gctgacgctg cgttggccag ccgtgtactg tctgtacacc gtcaccgtta ttggcatcat
    11161 agagcgcgct aagctgagaa cggcgtggga gcatattggc gccacagcca cctttactgg
    11221 tgctggaggc gttggcctgg ctgactcgcg accagacttc gttattttcc gttaatgtgc
    11281 cgtccggact gaacacttcc gcagccagtc ggggtcgggc aaaggttaaa gcatcgacgg
    11341 taatcgtctc atccatatgc ccccacatct gcgcgccaac cgcatccgga ctggtgagcg
    11401 tagtgaaaat cacattgtaa ttaacagtat tgggaatata ggagttcaca ggcacaatat
    11461 ttagcggcgt cgataagccc acaccttgcg attgcttaat ttccacagtc gcaaaaccct
    11521 gcgcatccgt agtaccggca tagactaaag acggttgcga tgttccgtag agtgtgccat
    11581 tcatctcgat tgcgccgcgg gttggatcat caaaacctgt agtctgcccc tgacgatttt
    11641 tccccatatc tttagtaatg gtgaacgcgg tatacggcac gggtgcatta ttcaaggcat
    11701 taaaggtgcg taccgtcatg gtgatcgtgt cacccacttt ggcctgcgct ctgctgcctg
    11761 cggaaacatc acgatcggtg gtcgcggcaa tctgggcgac catctcattg cccgaacaag
    11821 agagatagtt ttgcttaaag ccggaatagg tgtcgatgtt gcccgtggcc aggtttaccg
    11881 acgcgtgcga cgtctcttcc accgcactaa gatacgcttt ttgcaacgtt ggccagccat
    11941 attcggtgcc gatggcattg gctggatgag cggcaaacaa actgtgcaat gaactttggc
    12001 tcggaatatg acctaaacca cactcagcct gcatactgct attctgatca aacagcgccc
    12061 agtcttcgtt attttcccgc accgatccct gttcgttgtc ggtttcatcc gccagccgtg
    12121 ggcgtttaaa gatattgttc gcttcaataa tgcccaacat atggccccac atccgcgctt
    12181 tgtcggtatc ggggctggtg atcaccgtaa aaataacgtc tttctcatcc gaggcagtaa
    12241 aatcactgcg cattctcgcg gtgatgtgag ttttcacccc cacgccgcct ggctgggtca
    12301 gcgtcagagt ggcctctccg ttagcatcag tgatcccttc ataggtatgg gcgtcaactt
    12361 tgcgaacgtt actgccgcca tcaatttgta ccggatgcgt ctcccacgcg gcgtcagttt
    12421 gattcttacg gttaatgccg tcatccagat gcagcgaaaa gtagtagtaa gccagcggct
    12481 gatcgttgtt cttgctatcg gtaatcgtca gacgtaaaga agcctcttcc cccaccttta
    12541 ctttcatcgc ttttatcgtc gaatcatagc tgcccggcgt cagtaccagc ttcggttcaa
    12601 catcgggcgc ttctttatcg acacagctca caataaacgt cgtatcgtcc ggtttttgcg
    12661 cttcgctgcg gttcgaaaca tccgccgcat ggtgctggcc gctcatcccc gccagtgatg
    12721 accagtagaa attaccctgc atcggccaac cagccacatc ctgcactgac gttggcacca
    12781 ccgtcgccag cgcaccgaac tgacgcattc cgggcaaaat gtcgcaatgt ttatccgcgc
    12841 cgctccaggt aaaggtggac caggtttcat tgtgatcgtt aaccgtaccg ccttcattat
    12901 cgacttccgc cgccagtttt ggtcgactaa aagtgtaatc acgggccttc agcgtatcgg
    12961 gcatatgacc ccacattgta gcctgcggaa catccgggct ggtgaggacg gtaaagatca
    13021 ctgccgtttc actggcctga gcaatacccg cgaggctcgc gaccaacggc gtttttacac
    13081 cagggccgtt ggcctgggtg accgtaaccg tggcaacacc attcgcatcg gtagtaccac
    13141 gatattccgt cgcggtggtg gttaattcgg tcgtgcccag ttttacaggc gcagtattgt
    13201 tcaccacacc ctggcggttt tcagcatctt tgcgtttgat aacaaaagga atattcccca
    13261 caacattacc gacgcaatct ttggtggtga ccgttaacgt gatactgccc ccaaccgtca
    13321 ttttcgccgc acctgaggat tcgtcatagc ccgcagcgct gacggaaata aggtcaccct
    13381 gcgcgggcgc aatcttcagc gtcactttct gatcggcgag catcagaaca ctttcatagg
    13441 gatagcccgt cgaggtgagt aaatataaca gcggcatggt ctgctgggga tcaggctgcg
    13501 cccaggcgac cagcgaacgc ccggcaagtc cctgtgcaca aaacgagcga tctgcgactg
    13561 gctgtgggtc cagtaaggtg tccggcgtgg cggcatccgc ccaaactaaa gatacagccg
    13621 gtggcgtttg gttttcccaa cgtggggtgg caatgatatc gccttctctg tcttgcgggt
    13681 tggtgacgag caatttagag acgctaacat ccgcgttgaa ttcactcggt ttagcggtaa
    13741 acgctacctc atgcgtcttt gtaggatcga gaaagacact gccctggtag acgggtatgc
    13801 tggcgctgtc ggcggtgggc acggtaccgt taaattcgcc gaccgtatct gaagagtctt
    13861 gccatgcaga ttctgcccat gaccacccag gcagcattgc gcttatcagc aatgcgcata
    13921 acacaaggat tttattcagg gtcagggaca tgacagaaac tccaccggtt gttaaacgga
    13981 cgcttgtcgc atcaacatat tttgcttaat ccgggattaa gcaaaatatg cggaaacagc
    14041 attcccttgc gggaatgctg ctgagtacgc gttatttgtg tttgtaaata atgtgaagtt
    14101 tgtaaccctg taccccgtcg ccgttggtcg ggatggaaag tgcctctttt ccatcgcgcg
    14161 cagggccgtt aaggttggtt ctggcttcgt cgttggtcgc agggatgacc aaattgttat
    14221 tatcgaagct tgagttaacg atgccacccg aagtgccaag ctgttggctg ctgccagaaa
    14281 gtacccaacg gaagtcataa ttggcggtga cgtcagcatc ggtacccgca tcaaacttgc
    14341 catttttgtt ctcatccgag tacagtttgg ccacgtaggt tttgccaagt tcgattggcg
    14401 tgctaccgtt gataaggttg atactggtac catcaacaaa gatggcgact ttcaggttct
    14461 ggttaaccac cggccccggt gcaacgttat cgctgtcgct tccgccgccg ctggcagtag
    14521 aaacgtcata aagatgcagc gcagtcccga cgtttggcgt accggtctgc gtggttggcg
    14581 taatttcaat accgatataa cgaccacggt cggcatcggt aagagtgtaa ctggtcacct
    14641 tcgttaccag ctcttttgga tcacccccct gcgcatcctt gtagctcatc cagcggatag
    14701 tatcgatagt tttcgcgtta tcgcccaatc ccccaagatc cacgtcgcct tcggtatcac
    14761 caatttccca acttacggtt aatttatcac ccgagtaaag acgcttgtcc gtttcactgc
    14821 cttgcgggcg agcgcctttt agatcagaag tcaacgttac acggtcggca tctgtcgtag
    14881 cacttccccc ttccgaatag agccacggaa tggtcccctg gaaaatattg gtttcgccgc
    14941 tgtcgaccca ggcactgtct tcagccagcg ccacaggtgc cacacaataa cctgccagtg
    15001 ccagcgctaa tgcgatccgg gtcagaccgc gtcttttatc ctgcttcatt gatgctctcc
    15061 ttaatgaacc ttcagcgact ggccgactgg ccttacgcca atcttatttt ttggtataca
    15121 gcacgcgtaa accgtatccc tgcaagccat ctcccgcttg tgcgccatag gtttgcgccg
    15181 cttcacggtt agtggccgga atcacaatgt cctcattgga agtgttcgcc tgtgcaccca
    15241 cttcaccatt gatgctcttg ccggtaaatt cccacttgta gtcgtactgc accatctctt
    15301 cgtcagtcag tttttcgacc tgctggaaga caccgtcttt attgttgtcg cgccataaaa
    15361 caaatcgata aatgttgttg agcggcaacg gttctttatc tttacctatc aggttgctgg
    15421 gtttagccgc ccccacacga tagataaaag catttagccc accaatttct gccccgagga
    15481 aagtgacatc cacatagtta ctgtcatcgt agggcgcggc ttttgctttc cagcgatagg
    15541 tgccaggtaa ggtactggag agggtgacag tgcccttttc tttatcttgt ttggcaataa
    15601 aaacgcaggg gttatcggtc gagcagggct tgttggttaa cgtcactcgt ttgtcctgcg
    15661 cgtcgtaaag ctgaggcgta aggttatctt ccggcaacgc atcgccgttg cggtcggtgt
    15721 tatcgccaaa gctgtcggtt acatcgagat ccatcactac accatcaacg ccatctgcct
    15781 gtgcgctggc ttcgtggctg tggttgttct cttctggcac gttcgcgatg ttattaacaa
    15841 tcaactctat tttacggtcc tgctgaacgg tgatttccac cacgccagag ttctgtcggt
    15901 tgcccttttc atcttccagc gccagacgta ctcgccaggt attgagtttt tcttgttcgg
    15961 cttgatcgga actgtactgc caggcaggca gcaccaggtt ccagtggtta ttgttaccct
    16021 ctgtcagagt attctctttg ataaccggca tcaccagcgt gcgggcggca ttgtttttcg
    16081 cctgccagga ggccgcaacg attttgtaat gatgattaat gagtgcgtta atggtccact
    16141 tacatttttc cagaccgacg gccgcttcgg gagtgtcctt aatgacgcac tcagggtgct
    16201 cgttggtggc atccgctttc aaccacaggg tgacatcaag cggatctttt tctttgtatt
    16261 ccagaacaat aaagttgtta cgttcaacaa agtcatgacg gctacccatg agcgagtgag
    16321 ccgcgctcac tttgtcagga tcaagcaggt ctttgagcgg gacgccaaac tggtagttca
    16381 gcgttgcgtt aacagaaaga tcggtattat cgccagtgcc agctttgaaa tctgtaccga
    16441 caacaatgag cggaacgggc tggtatttca cacccagcgt gacagcgaag ggatctttct
    16501 ccagactatc ggtaccaaaa agcgcgactt cattcccata atattgttcg aagacaattt
    16561 ttccgccaag ctgcgggtaa gcgggtagcc aggcttcagc gcgaatatcc caaccgcgcg
    16621 cagggcgttc ttcataaaag tcgaaatctt cagagtcttt ccagtcagaa agtgggtgat
    16681 aatagttgcc tgagaatttt aaataatccg tccaggcttc ggcgcctaaa cctaaacggc
    16741 gatgtccacg ggtaaagtca taatcataaa atatatttcc acccaataga tatttatcaa
    16801 aattatacct taccccaagg ccaatattcc cgatcgtacg atcttctttt cgttgcgcag
    16861 aaaattggct gaaataaaca gtggtttgat tatcgtacca gggaacaaaa taatcgatgg
    16921 aactgccatc cagatcgccg ccctgaccaa tggagaggtt caaacggacc ttaccgtagg
    16981 gagagagtgt gtcttcaata taagattgtg cggttgaggt gatttgactt tgcagatagc
    17041 ttctggcctg agattccagc gcctcagggg tcaagttttc aaacccttgc gtggcgaagt
    17101 tgatgacgta atcggctccg cgctctttca gcgatttacc cttgttggtt tgttcatcct
    17161 gttgtgctgc ctggctacct aaatcaggta acgattcgtt ttgcttaaca tttatctctg
    17221 gcgccgcgaa agcagcacca gagataaatg ttagaataat acagcgtttc catcgcagca
    17281 tgcactttcc ctgtgacaat gatacagtca aaaataagct ttaatattgt at
//
LOCUS       DAZFRG010000097        17198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFRG010000097 DAZFRG010000000
VERSION     DAZFRG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.097"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..78
                     /locus_tag="ACHKUC_002508"
     CDS             7..78
                     /locus_tag="ACHKUC_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618752.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(75..200)
                     /gene="yqfG"
                     /locus_tag="ACHKUC_002509"
     CDS             complement(75..200)
                     /gene="yqfG"
                     /locus_tag="ACHKUC_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO6618753.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVVMAIESRFKYMMLLF"
     gene            complement(202..1650)
                     /gene="uacT"
                     /locus_tag="ACHKUC_002510"
     CDS             complement(202..1650)
                     /gene="uacT"
                     /locus_tag="ACHKUC_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO6618754.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSLGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            2086..4005
                     /gene="ygfT"
                     /locus_tag="ACHKUC_002511"
     CDS             2086..4005
                     /gene="ygfT"
                     /locus_tag="ACHKUC_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO6618755.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            4005..4493
                     /gene="ygfS"
                     /locus_tag="ACHKUC_002512"
     CDS             4005..4493
                     /gene="ygfS"
                     /locus_tag="ACHKUC_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6618756.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRIRQQRIAASGAN
                     PL"
     gene            complement(4529..5896)
                     /gene="ghxQ"
                     /locus_tag="ACHKUC_002513"
                     /gene_synonym="ygfQ"
     CDS             complement(4529..5896)
                     /gene="ghxQ"
                     /locus_tag="ACHKUC_002513"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO6618757.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(5932..7248)
                     /gene="guaD"
                     /locus_tag="ACHKUC_002514"
     CDS             complement(5932..7248)
                     /gene="guaD"
                     /locus_tag="ACHKUC_002514"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO6618758.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVYNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(7266..8666)
                     /gene="xanQ"
                     /locus_tag="ACHKUC_002515"
     CDS             complement(7266..8666)
                     /gene="xanQ"
                     /locus_tag="ACHKUC_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO6618759.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(8831..11701)
                     /gene="xdhD"
                     /locus_tag="ACHKUC_002516"
     CDS             complement(8831..11701)
                     /gene="xdhD"
                     /locus_tag="ACHKUC_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO6618760.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(11698..12477)
                     /gene="ygfM"
                     /locus_tag="ACHKUC_002517"
     CDS             complement(11698..12477)
                     /gene="ygfM"
                     /locus_tag="ACHKUC_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO6618761.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQNLELDWIDWDNGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(12528..13856)
                     /gene="ssnA"
                     /locus_tag="ACHKUC_002518"
     CDS             complement(12528..13856)
                     /gene="ssnA"
                     /locus_tag="ACHKUC_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO6618762.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(13859..16957)
                     /gene="ygfK"
                     /locus_tag="ACHKUC_002519"
     CDS             complement(13859..16957)
                     /gene="ygfK"
                     /locus_tag="ACHKUC_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO6618763.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWEEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTTLKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLSDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSITVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNINSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         4347 a         4364 c         4411 g         4076 t
ORIGIN      
        1 agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgtcga
       61 caggtaaaaa ccatttaaaa aagcagcatc atatatttga agcgactttc aatggccatt
      121 acaacacagt cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata
      181 gcgcgcatta aaagattcat attattctcc atgctcattt ttcttcagct tgcgcatcag
      241 aagccacata cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag
      301 atcagcatga tgctgatagc cattaaagaa gacgttcagc acaacggcgc taagggttgc
      361 gagcataatg ccgctatgca gcaacggttg cagtacggcc ggtaacttag aaaagaaatc
      421 gtgagagagc gttggcgtca tgccaacacc gagactgatc gccacaatat agaggttgta
      481 acggttagtg gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc
      541 gaacatcacc agccccgcgc cgcccagcac aaattgcgga atcgaggcca ccagcaccgc
      601 cattttgggc accatgccga acaggatcaa aatgataccg gaagcaatgc acacccagcg
      661 gctatgaacg cgcgtcacgc taaccaggcc aacgttttga gaaaatgacg tgtgggggaa
      721 gctgttaaac gttccgccta tcattgtccc tacaccatcg acacgcagcc cgcgaataat
      781 atcgtgtgaa gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga
      841 ctcgataaac acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat
      901 cggcatacca aatgacatcg gcgtgacaat cgcaaaccat gaagcatcat gtagcccgga
      961 taaattgact tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac
     1021 ggcgacgttg gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac
     1081 ggcaaaggag atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc
     1141 ggcccagtca atacccacct gaatgatgct aagcccgata gaagtaatca ccacaccggt
     1201 aaccagtggc gggaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat
     1261 aaaacccgcg gcgatagtgg caccgaatat ccccagcagg ccgatatccg ggttcatacc
     1321 aatggctatc attggtgtta cagcggcgaa ggtcaccgac ataatcaccg gcaggcggat
     1381 ccccataaag cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag
     1441 atccgagcta atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat
     1501 aagaggaaca gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat
     1561 cagctttcct agtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga
     1621 tgagggaagt tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa
     1681 aaagcaaccg tttacgttca tgaaactgtc taaacacatg ctcgtggagt gccagttttt
     1741 gcgaggaagg ggagttagag ataggaattg cggaggcaat cacataatcg tggttaaacc
     1801 acgaaaatga gaagagaacc tgacaatgga attatctgta attttctcat attgatattt
     1861 cattaatcat tctggcatgt cgcactctcg catttaatcg tttttatctg gatagcattc
     1921 ttttgatcgg caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac
     1981 tcagttgcaa aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat
     2041 cgcgaaattt gagaagacat aactcattaa atgaaaggga tgcaaatgaa taagtttatc
     2101 gctgctgaag ctgcggaatg tataggctgc catgcctgtg aaattgcctg tgcggtagca
     2161 cacaatcaag aaaactggcc gctgagtcac agtgactttc gaccgcgtat ccacgttgta
     2221 gggaaaggcc aggctgcgaa tccggtggcc tgccatcact gcaacaatgc cccttgcgtt
     2281 acggcttgtc cggttaatgc tctgactttc cagtccgata gcgtacaact ggacgagcaa
     2341 aaatgtattg gttgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc
     2401 gatacgattg cacagaaatg cgacctttgt aaccagcgca gttccggcac gcaagcctgt
     2461 attgacgtct gcccaacaca ggcgttacgg ctgatggacg ataaagggtt gcagcagata
     2521 aaggtggccc gccagcgcaa aacggcagca ggaaaagcgt catcagacgc tcagccatct
     2581 cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt
     2641 gaacggaaaa cccactttgg cgaaatctat tgcgggctgg atccacaaca agcgacttat
     2701 gagagtgacc gctgtgttta ttgtgccgaa aaagctaact gcaactggca ttgtccgctg
     2761 cataacgcta ttccggatta catccgtctg gtacaggaag gaaagattat tgaagcggca
     2821 gaactttgcc accagaccag ttccttaccc gaaatctgcg gcagggtatg tccacaggac
     2881 cgtctttgtg aaggtgcatg tactttgaaa gatcactctg gcgcagtctc tatcggtaat
     2941 ctggaacgct acatcaccga tactgcgctg gcgatgggct ggcgtcccga tgtcagcaaa
     3001 gttgttcccc gtagcgaaaa agtggcggtg attggcgctg gacctgcagg attagggtgt
     3061 gctgatattc tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca tccagaaatt
     3121 ggcggtatgc tgacttttgg cattcctcct ttcaaactcg ataaaacggt attaagccag
     3181 cggcgagaga tattcaccgc aatgggaatc gactttcatc ttaactgtga aattggccgc
     3241 gatatctctt tcaacgaatt aacggcggaa tatgatgctg ttttcctcgg cgtggggact
     3301 tacgggatga tgcgagcaga tctgccgcat gaagatgcgc ctggtgtcat tcaggcgtta
     3361 ccgttcctga ccgcccatac ccgccagctc atgggattgc cggagtctgc agagtatccg
     3421 ctgacggatg tggaaggtaa gcgagttgtg gtattgggcg gtggcgatac gacaatggat
     3481 tgtttgcgga cttccatccg ccttaatgcc gccagcgtga cctgtgcgta tcgtcgtgat
     3541 gaagtcagta tgccgggctc gcgcaaagag gtggtcaatg cccgcgagga aggtgttgag
     3601 tttcagttca atgttcagcc gcaatatatc gcttgtgatg aagatggacg cttaacggcg
     3661 gtgggcctga ttcgtaccgc tatgggtgag ccggggccgg atggtcgccg tcgtcctcgt
     3721 ccggttgcgg gttcagagtt tgaattgccc gccgatgttc tcattatggc ctttggtttc
     3781 caggcacata ccatgccgtg gttgcagggc agcggaatta aactcgataa atggggcctg
     3841 attcaaaccg gcgacgtcgg gtatttacct acccagacgc atctgaaaaa agtctttgct
     3901 ggtggtgatg cagttcatgg cgcggatctg gttgtcactg caatggccgc aggaaggcag
     3961 gcggcgcgcg atatgttaac tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta
     4021 tcgttaatcc ggctgactgt attggctgcc gtacttgtga agtggcctgt gtggtcgctc
     4081 acccttcaga acaggagttg aatgccgata tctttttacc ccggctgaag gtacaacggc
     4141 tggatagcat cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg
     4201 cttgccccgt gggggcgctg acgatgggcg agcaggtggt gcaggccaat tctgcccgtt
     4261 gtattggctg tcagagttgc gttagcgcgt gcccgtttgg gatgatcacc attcagtcat
     4321 tgccgggtga tacccggcaa caaatcgtga aatgcgatct ctgtgaacag cgggaagagg
     4381 ggccagcctg cgttgaatct tgcccgacac aggcgttgca gttgctgact gaaagagaac
     4441 tcaggcgtat ccgccagcag cgtattgctg ccagcggcgc gaatcctctt tgataaaaaa
     4501 ctccgcgttg aaaggcgcgg agaatctatt agatagccca accacccgcg taaaacgcga
     4561 ccagtgcggc agtaatgatc accgtaccaa tattcagctt ttgccattcg cgcgcaaaga
     4621 cgcgacccac caccagtgtt acaaagccca gcataatacc ggtaacgata ttacaagtca
     4681 gaacgatgaa cacggcacac accaggccag ccattgcgtc aataaaatcg ttgaaatcca
     4741 gcttcgagac gttgcttaac atcagcaaac ctacgtacat cagtgcggga gctgtggcat
     4801 aaccggggat cagaaacgat aacggtgata aaaacagaat catcaggaac aacacaccaa
     4861 ccacggttgc ggttaaaccg gttttccccc cggcggcggt tcctgccgct gattcgatat
     4921 aaaccgccgc gggcgctgcg cccaccaggc cggagaatat tgaacttact gagtcactgg
     4981 tcagggcttt gccgccgttg atgatctggt tgtctttatc caacaaattc gcctgaccgg
     5041 cgacggcacg gatggtgcca gtggcgtcga acactgcggt catcaccaat gccagtacac
     5101 tcggaagtac agttggctgg agtgcgccca taatatcgag gctgaaaatc agagacttac
     5161 catcttcgcc agtcaggctt ggcattgcca ccagaccgtg gtacttcacc gctgggtcaa
     5221 agattaagcc gatgatcgaa attgcaataa tcaccaacaa gatcccgccg ggtacgcggc
     5281 acttttccag accgaagatg accgccagcc ccagtaagct catcatcacc gggaaggagg
     5341 taaaggcacc gagcgccact ggcaagcctt caatcgggtt tttgataacc attcccacac
     5401 cgttagcagc aatcagcagc agaaacaggc cgataccgat acctgtaccg tgagcgatcc
     5461 ccatcggcaa attacgtaag atccaggtac gcacaccggt tacggaaata gcggtgaaga
     5521 tgacgcccat cagaaagact gcgcccagtg cgacaggaac gctaatttgt tgcccgagta
     5581 ccagactgaa tgcggtaaac gccgtcaagg aaatagcgca accaatcgcc attggcaaat
     5641 tggcccataa ccccatcagc aacgagccga agcccgcgac cagacaggtg gcaacaaaca
     5701 cagctgcggg aggaaaacct gctttgccca gcattcccgg aacgacgata acggaataaa
     5761 ccatggccag aaaggtcgtt aagccagcca gtacttcctg acgaacggta ctgccacgag
     5821 cggtaatttt aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta
     5881 ggatgtctcc agacataatg atgtcctcac gaggatgtta aagttttatt attagttgcg
     5941 ttcgtacacc agacgaccat caacgtaggt gcggtagatc gaacggtcat cgcccaacgt
     6001 catcatcacg aacaatttgt cgactaaaga aacagagttg tcatagcgca gctgctgtag
     6061 cggagtggca gtgggttcca tcaccacgaa atcagcctct ttgccaggta aaaagttgcc
     6121 aatcaaatcg tcaaggccca gagatttcgc tccgccgagc gtggccaggt aaaacgcttc
     6181 atatgccgag aggcgatagc cttgtaattg caataccttg taggcttcgt tcagcgtttg
     6241 cagcatgtta aaagttgttc cggcaccgat atccgttccc atgcccactt tgactttctt
     6301 ctgccatgct tttttcaagt tgaataagcc gctgccgagg taaaggttgg aggtcggaca
     6361 gaaagcaatg ctggatttgg tttcgctgag acgatcccac tctttttctt cgagatggac
     6421 gcagtgagca aagacacaat ttttaccggt caggccgtac tgatggtaaa catcaagata
     6481 accatcatgg tcaggataaa gcgatttcac ccaggcaatt tcatctttgt tttcacagag
     6541 atgggtatgt acccatgtat ccggatattc ttctttcagg cgttgcgcca tcgccatctg
     6601 ttcaggagat gaggtagggg cgaagcgtgg cgtaatcgca tacaacagac gaccattttt
     6661 gtgccagcgt tcaatcagtt ctttgctttg gtgatagctg ctttcggcag tgtcgagcag
     6721 ataatccggt gcgtttcggt ccatcataac tttaccggca atcatacgca tattgatatg
     6781 actggcggct tcaaacagcg catcaacgga ttgtggatga acagtgccaa acaccagcgc
     6841 cgtggtggtt ccgttacgta aaagctgctt gatgaagaac gccgacattt cgcgggcgta
     6901 ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt ttattcaacc actccagcaa
     6961 ttgttcacca taggccccca ccatttcact ttgcggataa tggatatgtg tatcgacaaa
     7021 gcccggtact atcagtttgc cgcgatagtc gcgcacgcga atggtgtcag gaatttgatg
     7081 ctttccgtct tcccattcgc caaaccattc cacttttccc tgtttaatga gtaataaacc
     7141 atcctcaata aaccgcagcg cagaggcaat ttcttccggg ttatagaccg tacgggtgac
     7201 atcaataaaa ctgcctcgta ccgcttttaa cgtgtgttct cctgacatca ttgactcctt
     7261 tactgttaat ccatctcttc cgctgaggta ataccaggca gaacgttttc ctgtcggtag
     7321 ccaccaggga ggataatatt taataaaatc gcagttaacc cgccagcaca aatagggttt
     7381 tcaactaata cataaataga ggctggcaat attttaaaaa tttcgggatc gtaggagaca
     7441 ccaagcccta aacctaaaga cgtggcgaca ataagcgttt cacggcgctt taaaccgttg
     7501 gtgatgatga tgcgaatccc tgcgatggca atcatggaaa acatcaacgt cattgcgcct
     7561 cccagaactg ctgagggaat ggtcgtgaag aagccgccaa tcatcggaaa taagccgagg
     7621 ataaccagca ttaccgcgat ggttcgcccg acataacgtg aagcgacgcc agtcatctga
     7681 ataaccccat tattttgcgc aaacgtggtt aatggtaatg aaccgacagc ggaggcgata
     7741 acagaaacca gaccatctgc cagcacgccg cctttcagtc gggactgata ctcttccccc
     7801 tgaatggggc ggcgggaaac cattgctgtg gcggtgatat cgccgacagc ttccagcacg
     7861 ctaagcagat aaatcgtgcc aaccaccagg aactgatgga agctaaaact aaagccgtat
     7921 ttgaacggat gcgggatggt gattaacggc aaattgcgca tactgctgaa atccaccatg
     7981 cccaggcata acgatgcgat atagccgaca catagcccaa tggcgatccc acccatgcgt
     8041 agcaacggac tgcggcagca gttaaagccg atcaccacaa tcaaaaccaa taagccaacg
     8101 ccgagatgct cgtaattgcc gaacgtaccg ctgcttttag ctgcaaaacc accgccaaaa
     8161 tcaataatgc caactttaat cagacttaag ccgatcatca gcaccacaat gccgctgacg
     8221 gtaggcgtaa taacccgacg taaatagggc aggataaaag aagagccgac aaccagaaat
     8281 gcgccaacga aggagacgcc gagtagcgac gacatgatta actcttcgtg aaaaccgtcg
     8341 cttttcatgc tgctacccag cgcaatcatg accgtaacaa atgaaaaatt gactgactga
     8401 attgaaagta aaccagaacc gacgatgccg tagcggttta cttgtaacca ggtaccaata
     8461 ccagaggcga tcatcgccat agaaacaaga taggcagttg tttcagcgga aagctgtaag
     8521 gccgcaccca cgattaacgc gggggttacc atcggaacga aaattgccaa cagatgggta
     8581 atggcaccta cgagagcctg atgaaaggga gggcgatcct ccagttcaaa tataaggtca
     8641 gaacctgcat ggtttatatc agacatccct tcactccttt taaagtaaat ttgatcatct
     8701 gaattagtaa aataaattcc cacgaatttg gggcgcagga cgccttgaga acggcttatc
     8761 tcattttcgc agtcactatt tagtcatcgt tcaaacagag aaacaggccg ccgaagcggc
     8821 catttattac ttatattttt tccagcgcgg tgagtatttt ctccggtgtg aaatgccatt
     8881 cgcgtaacca gatgccgcag gcatcgtgaa ttgcggtagc aatcgccgga gctgcgccat
     8941 ttacaccgat ttccgagatc gatttcgccc cgaatgggcc gactttatcg tcgctcggca
     9001 ccagcacagc gcggaaatca cgcggaatgt caccaatttt cggtgcgccg taactgcgta
     9061 aatcacgcgt taacgggtga ccttcggcgt cataaatgat ctcttcgctc atactgtggc
     9121 cgatagctcg cagagtggca ccgtagattt gtcccaacgc taactctgga ttgaccggtg
     9181 taccgcagtc cagcaaggcg tagaatttat ccaggcggat ttcacccgta cgcgtgttga
     9241 cggcaacttc agcgaagttt gcgccatacg ggaaggcgaa atcaggtgtg atataactgc
     9301 cagttcccac cagtgaacca aagccggtac cggtttcgcc tttatgggct atctctccga
     9361 aactaacttc gcctttcttg ccgcgaacga cgcccggcgt tgctagttga acatctgcca
     9421 ctggctcacc caatatttgc gcgccgtgga acaggatttt ctcccgcaga ttttccgctg
     9481 ccaaacgcgc agcgttaccc gagaagcaag taccggacga ggcatatgcg cctttatcaa
     9541 acagtgcatg atcggtatca ccagagataa catgcacgtc ctgcgggggg cagtgcagca
     9601 cttctgctgc cagtttggtc accacagtat ccagaccagt accaatatcc gcaccaccgg
     9661 aatgaacgat aaaggtgcca tccgattcca gtttgatcat gcagttagcc tgatcgatat
     9721 ccgggatacc cgatttctgc atgataatgg cgacgccgcg accgatgtgc cagtcaccat
     9781 tttgcggttt tggtgaagac cattggatca tctcgcgacc ctgacgcagg atctcttcca
     9841 gtgcgcagct ggcggcggaa ggaacggagg tcggcgcttt accttcaccg attgcaccga
     9901 gaattttcag ctcttgccct tcgtgtaccc ggttacgttc gataatttcc agttggtcga
     9961 tctgtaactg ttcagccagt tccgctaatg ccatggtgat agcgaagtta cctttcggtg
    10021 cgccataacc ctgataagca ccatttgggc aaatgttgct gtagtaggtg gtgacctgaa
    10081 aatcgacgtt atcgcatgga tataacggca gcgacagcgc cggtccgtta cacggtacgg
    10141 tgagtgagtg gttaccgtaa ggacccgtgt tggcgcggaa atccatcttc actgccgtca
    10201 ggcgaccatc ttttttcgcg cccagtttga cggtgacttt cgcgacgtga cgagaggtgt
    10261 tagcaataaa ctcttcttca cgggtgtagc ggaaaagtac cggacgcccg gtcacgcaag
    10321 ttgcccaggc gcacacttct tccagcagga tgtcctgttt agagccaaaa ccgccgccta
    10381 cacgttcttt aatgacatga actttatgtt gtttcatgcc cacgaggcgc gcgacctggc
    10441 ggcgtaagtg ccatggtacc tgggtggagg cgtggatcac cagacgatcg ccgtccatac
    10501 gggtaaagca gatatgtgtt tcagtcgggc actgctgcgc ctgcgttgag ttataggttc
    10561 gctcaatgat cacatcggca tcggcaaagc ctttgtccat atcgccaata tgaccatgaa
    10621 tactggcagc gatatttttg cgtgggcgag aaccgatcgg gaagttgatg atcatatgct
    10681 cgccgcgctg ggccgcatgg ctgttatcat cttccagagt atctggtgca ccagcaacat
    10741 acaccaccgg ttcatcgtgc acgacaggcg catcttccgc cattgcctcg tcaatcgaca
    10801 ttaccggctt aagcacttca tattcaacgt cgatgagctt cagtgcttcg agcgcaatgt
    10861 cttcactttc ggctactacc gcagcaacgc gatcgccgac gtgacgcatt ttcttgccga
    10921 acattcggcg gtcaagcggt gacggttccg gtgcgctctg accacccggg gtatagtaaa
    10981 tatccgggca attcaggtga gtaataacgt gaacgacgcc cggtaaggct tcagctttgc
    11041 tgacatccag atgagtaatc agtgcgtgag cgtgtgggct acgtaacatt tttatgacgc
    11101 aggcgtcagc cgttacgcga tcttcaacat agcagggttt cgcctgcacc attttcgcgg
    11161 catcagtttt aggataatgc ttgccaatga cttcaaggtc gtcacggaaa gtcggagcga
    11221 tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc cagttcaatg acctgatagt
    11281 attgctgcca gccagcatcg cggctaaaca gacctgagag tgcgtcgtcg atctcttcgc
    11341 gggtaggtgc gtcgatgcga tcgagaagat cggtgataat cagagccgca gctggatcgt
    11401 tataaccaga ctgcaccacg ccaacatcaa ccatggcctg ttgaaccaga cttaactcat
    11461 tccatttgcc cagagattct gcggtacgaa tatctgcctt ctctaactgt gcggcgataa
    11521 gcaaggacgc gttaacgata ttaccgttaa agataattgc gtcagaaccg gcaaacccaa
    11581 aaccatcatc actgttgcgt acagagtgca ttcccatgtt aaacaacagc ttttgcacgt
    11641 tttcgcctgg attaacggtt agctcctgag gcgcgccatt taaagtaaag tggatgatca
    11701 tacggcttcc tccccagcct gttggcagtc ggcatacagg tcggctacca gaactcccgt
    11761 gatatagcgt ttataggcca cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc
    11821 gacagcctgt tcaagtgcat tgccttccag attttgagtt tcaacatcgt gcagacgcag
    11881 tgctttactg gcgacgccat ccagcgcaat tcgcataccg tcgtggtctg tcattgcaac
    11941 ggctgccgtc acgacggtta aacctgcctg agaacggcta attttgcgag tcgcacaggt
    12001 gcgatacgga tctttaatga taatttcggt taacaggcga tcgcatgggc aggccaggta
    12061 gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt tcagcatcca gtgccagcag
    12121 gacgggaagc agcaccgact cttcctgccg ggcggcgatt tcaccaccaa tggtcgactg
    12181 attacgaaca tggcgtgagt aaacaaaacc gagagcttca cgcagtgctg caggaataaa
    12241 tcgtgcatca cgcagtgact gcaagcgaga cattgcgcca attcgtagtg caccgttatc
    12301 ccagtcaatc cagtccagct ccagattctg caaggaaatg gcaatctttt tatcggtacg
    12361 ggttggtgta gcgttgagtt tgctgccccc ggcgaaccag acggcttcat cctggtagcg
    12421 gcgcttcagt tccagcgctt gttcgacaga gtcgggtctg aaaaattgtt caatcatcgt
    12481 ctttccttaa tttcccctct tctctgaaag aagaggggag ctgttattta tgccagcgca
    12541 tccatccgac gccacatact ggcagcggct tttctggctt gcgcataaat ggaatcgcaa
    12601 tcgaagttaa actgacggtc ttcatagacc atcacaccat tgaccatcac gctgtgaacg
    12661 ctgcctgagc ccataccgaa agcgatatgc ccggcgatat tgtctgccag cagcggcgtt
    12721 ggcgagttgt aatcgcaaat ggttaaatcg gctttgtaac cggcttccag aagcccaaat
    12781 ttcgcgccaa agttgcggct catcagttcg ttgccgttag ccagtgcttt ggcaaaactg
    12841 tcaggccaca gcgggccacc cgcatcgcga tgtttaaaga aggcaaattt catctcttca
    12901 aacatgtccg aaccaatgcc gtccgttccc aacgccagat tgcggatgtc gctaaggtga
    12961 tggttgtagc cgacatggtt gttcatgttt gaacgggcgt tatgcaccag gaacgcatcg
    13021 cgctgattga gtagggcgat gtcatctttc gacaagtaca gcccatgagc gaccagcgtt
    13081 ttgctgtcga tgagatcgaa ttgcgccagt cgtgccagca ggtctttgcc gtaccaatgg
    13141 tgactgtagg aaacgtcgta aaggtcttcc gcagcgtgaa tatgcaaacc acgacctgtg
    13201 cttttcacgg cttcacgcag catctccaga ccggcatccg gtacggtaaa cggcgcgtga
    13261 gcgccgatat gtgcttccac cagatacggc tcggttgctg ctttcttcgc ctcatcaatc
    13321 tgacgggcga agcggatgtt ttcttctaca ccttcctgca actctttgat gccgctgtta
    13381 cggtcagtag tttcaaaaca ggtcatcgcg cgcaggccaa cttttaaaaa tgcgtcgcgc
    13441 aatgtggaga gcgacccgcc gatataggct ggagaggcat ggtgatcgat aaccgatgta
    13501 catccgctct taatcgcttc cagggaacaa atcagtccgc tgtaatagag cgactcttca
    13561 tcaagggcgc gatcgagccg ccaccagaga tttttcagcg ttgagatgaa atccgggcag
    13621 ggggcgatgt ttgccataat tccgcgggaa agccccgagt aaaaatggtt atgcgagcag
    13681 acaattcccg gcatcacaat ccgaccatgc atctctttgt agctggcatc ggggtagcgt
    13741 tgcgtcaggg catcgccgat agcgacaatc acatcatttt cgatggcgat atcaacgcct
    13801 tcctgcactt tcgccgggtg taactgcact gcggtgacat tcttcagaat caacatgatt
    13861 acacctccac gcggcccagc agataatgat gatgctggtg gacatggctg atgatacggc
    13921 acatatcgtt cagctccggt ggtacgttgt tgaactgacc ttcgctgttg atgtttaaca
    13981 cccagctttg gttattcaga cgtacgcgta cccggcaatc ttccacaagg aagcctgggt
    14041 tgctgctgtt atcaaagtct tgcgacaggc tgaagacggt gattttgtct ttgtacggtt
    14101 taccgttcca cgggcagaat tgggcgcagt tgccacattc gttacagtaa gcgtcgaggt
    14161 gcagcgtctg gaaacggttc tggaagcctg ggaccgtaat ggatacgttg gcgcggttcg
    14221 ggcagacatc cacacacttg ctgcaaacgt agttacattc aaggcagcga gcggcttcct
    14281 gcgcgacaaa cgcatcacga tcgtcactgt tcaccagagt gacagagata tcgcctttac
    14341 gttgatagat ttccgccgga ttgacgttat tccagtattt atcgttctgg tgggaacgga
    14401 tattttcccg actcaggatg gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa
    14461 tggaggatgg tccgcgctgt acgtcgccga tcataaagac gtcactcaga cgcgtttcgc
    14521 cattatggtc gacgtctggc cagccgtttt tgtccagcgg cacgcccatc gcattcaggg
    14581 cttcagtatc ctgctgttca ccaatggcgg tgatcaggct gtctacatgc agtgttactg
    14641 tttcgttggt ttcaaccgga cggcgacgac ctttctcatc aggttcgcca agcgacataa
    14701 cgcgcaaggt taaggtgcca tcagcatcga aacgttccgg attattcagg aaacggaact
    14761 ccacgccgtc gtgcaacgct tcttcatact cttcgcgcca tgctggcatc tcttgcagtg
    14821 aacgacggta aacgaccgtt gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc
    14881 agtccattgc ggtgttaccc gccccgacaa cgaccacatg tttgcccagc ttgagcgtgg
    14941 tgcccttgtt gtattcacgc aggaagggga gtgatttcca gacattttgg ttgtcgcccg
    15001 ccagtttcac accgctattt ttatcggtac cggtggcaat cagaacatag tggaagccct
    15061 gattttttaa ctgttcaacg gtcaaatcgg gtgagcaacc gtactcaaat ttcacgccgt
    15121 gatcggcaac aaaatcgata tcgtgctgaa ttaactctgc aggaatacgg aactgaggaa
    15181 tgatattttt caccacgccg cccgcattgg cttcgcgttc aaacagcgta accggatggc
    15241 ccgctctggc aaggaagtaa cctgcggcca gacccgccgg acctgcacca atcacggcaa
    15301 ccggatggcg tgaaccagaa ccggctggtt tgtgccagcg ttgcttatat tcctcccaac
    15361 ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg taatccaggc
    15421 gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggcg ggcagggcgt
    15481 tgcgttgata gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc agacggatgt
    15541 attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg tagcaatcgg
    15601 tcagcggcag gtcttcagcc acttcaatac gctcttctgg cttccagtgt ttctgggtgt
    15661 attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca acatggtcaa
    15721 gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc agatagccgc
    15781 caggtttcag caggtcggtt gccatagtaa tagggcgaat accagtatca aaaatatcgc
    15841 ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc
    15901 gagagagaac tgctgcaacg ttgatggaga gcgggaacag cgcacggcct gacatataca
    15961 tctcttcacc gggcaatgcg cctttattgt tgatggtgcc gagagtgtta gtcagtttta
    16021 cgccaaagcc gagtgatttt tcttttgcca gtgccatcag gcgttccagc atttccagtg
    16081 cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagccgatg taaccgaaac
    16141 cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg ttgagtttca
    16201 caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg atttcatgcg
    16261 gaggacagcc gtgcatggtg gagagggtaa ccccttgtac catgctggtg gggatgcgag
    16321 cgggtaaggc ttgcaagctt tcgcgttttt cctgcaatcc gtggcgggct aaaaatgcct
    16381 catcctggag tagcttatta agtgcatcgc ggtattgagc gaatttcgga tggtcagatg
    16441 cgtccatcat attgtcgatg aactgctgca tcggcggttg cttaatacct tcgaggttgt
    16501 aaccgacgct catattaaag atgaacgatt taccggaatc agaaggctgg aacatcgctt
    16561 cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta agcagggtaa
    16621 attcggtaga ccattcggtg ttaaagcact cgtcttcggc atcgatacag ggcttttcca
    16681 gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg ccagtcagcc
    16741 aggaggtgac gatgttttgc gcaagttgcg tgtgcgggcc agcggcaggg ccgacgggag
    16801 tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacgggtgag taaaactgtt
    16861 gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa
    16921 gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta gcttatacag
    16981 atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga gcaacaatca
    17041 caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa
    17101 tggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact cctgaaaatt
    17161 attattgcca gcccatttat cactttttct cttttctc
//
LOCUS       DAZFRG010000098        17054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFRG010000098 DAZFRG010000000
VERSION     DAZFRG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.098"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..317
                     /gene="bssS"
                     /locus_tag="ACHKUC_002520"
     CDS             63..317
                     /gene="bssS"
                     /locus_tag="ACHKUC_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO6618764.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            432..1550
                     /gene="solA"
                     /locus_tag="ACHKUC_002521"
     CDS             432..1550
                     /gene="solA"
                     /locus_tag="ACHKUC_002521"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO6618765.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPAHDNTLLITG
                     LSGHGFKFASVLGEMAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1598..1711
                     /locus_tag="ACHKUC_002522"
     CDS             1598..1711
                     /locus_tag="ACHKUC_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO6618766.1"
                     /translation="MRRLVNYLINNIREHLMLYLFLWGLLAIMDLNYVFYF"
     gene            1972..2538
                     /gene="yceJ"
                     /locus_tag="ACHKUC_002523"
     CDS             1972..2538
                     /gene="yceJ"
                     /locus_tag="ACHKUC_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6618767.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGTQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKYDTLKRMLGKSSSDYGV"
     gene            2542..3117
                     /gene="yceI"
                     /locus_tag="ACHKUC_002524"
     CDS             2542..3117
                     /gene="yceI"
                     /locus_tag="ACHKUC_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO6618768.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3159..4211)
                     /gene="trhO"
                     /locus_tag="ACHKUC_002525"
     CDS             complement(3159..4211)
                     /gene="trhO"
                     /locus_tag="ACHKUC_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO6618769.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPDEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4436..5356
                     /gene="lpxL"
                     /locus_tag="ACHKUC_002526"
     CDS             4436..5356
                     /gene="lpxL"
                     /locus_tag="ACHKUC_002526"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO6618770.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5528..6754
                     /gene="mdtG"
                     /locus_tag="ACHKUC_002527"
     CDS             5528..6754
                     /gene="mdtG"
                     /locus_tag="ACHKUC_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO6618771.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            6837..7211
                     /gene="msyB"
                     /locus_tag="ACHKUC_002528"
     CDS             6837..7211
                     /gene="msyB"
                     /locus_tag="ACHKUC_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFO6618772.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(7212..7439)
                     /gene="yceK"
                     /locus_tag="ACHKUC_002529"
     CDS             complement(7212..7439)
                     /gene="yceK"
                     /locus_tag="ACHKUC_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6618773.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDVHHGPYE"
     gene            complement(7611..10154)
                     /gene="mdoH"
                     /locus_tag="ACHKUC_002530"
     CDS             complement(7611..10154)
                     /gene="mdoH"
                     /locus_tag="ACHKUC_002530"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO6618774.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEVKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10147..11682)
                     /gene="mdoG"
                     /locus_tag="ACHKUC_002531"
     CDS             complement(10147..11682)
                     /gene="mdoG"
                     /locus_tag="ACHKUC_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO6618775.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12076..13233
                     /gene="mdoC"
                     /locus_tag="ACHKUC_002532"
     CDS             12076..13233
                     /gene="mdoC"
                     /locus_tag="ACHKUC_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO6618776.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13241..14722)
                     /gene="clsC"
                     /locus_tag="ACHKUC_002533"
     CDS             complement(13241..14722)
                     /gene="clsC"
                     /locus_tag="ACHKUC_002533"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO6618777.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPQLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFIHHLVDGTLSLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGWINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(14664..15197)
                     /gene="ymdB"
                     /locus_tag="ACHKUC_002534"
     CDS             complement(14664..15197)
                     /gene="ymdB"
                     /locus_tag="ACHKUC_002534"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO6618778.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15292..15603)
                     /gene="ymdA"
                     /locus_tag="ACHKUC_002535"
     CDS             complement(15292..15603)
                     /gene="ymdA"
                     /locus_tag="ACHKUC_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO6618779.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSAVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15724..16056)
                     /gene="csgC"
                     /locus_tag="ACHKUC_002536"
     CDS             complement(15724..16056)
                     /gene="csgC"
                     /locus_tag="ACHKUC_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO6618780.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNIPPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16115..16573)
                     /gene="csgA"
                     /locus_tag="ACHKUC_002537"
     CDS             complement(16115..16573)
                     /gene="csgA"
                     /locus_tag="ACHKUC_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO6618781.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16614..>17054)
                     /gene="csgB"
                     /locus_tag="ACHKUC_002538"
     CDS             complement(16614..>17054)
                     /gene="csgB"
                     /locus_tag="ACHKUC_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO6618782.1"
                     /translation="LFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAII
                     GQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQG
                     AYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4273 a         4351 c         4039 g         4391 t
ORIGIN      
        1 tcagataaaa acgcattgaa cctcgaataa cgttgtctag taacacgaat tagggggcca
       61 tgatggaaaa aaataatgaa gtcattcaga ctcatccgct cgtagggtgg gacatcagca
      121 ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta ccagacccca aataagtccg
      181 agcaggaagg gactgaagtt ggtcagacgc tctggttaac cactgatgtt gccagacaat
      241 ttatttcgat attagaagca ggaatcgcca aaattgaatc cggtgatttc caggtaaacg
      301 agtatcggcg tcattaacat gacatgacca tccacccaaa aggcacctca ccggtgcctt
      361 taccatttct gggttgtata tctgcattca gttaattact ctgcttaaac catgagagca
      421 gagagaaaaa aatgaaatac gatctcatca ttattggcag cggttccgta ggcgctgccg
      481 ccgggtatta tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc catatgccac
      541 cacatcaaca cggcagccac cacggcgata cgcgattaat tcgccatgct tatggtgaag
      601 gtgaaaagta tgtcccgctg gtcctccgcg cgcaaatgct gtgggatgaa ctttcccgcc
      661 acaacgaaga tgatcccatt tttgtacgct ctggtgtcat taacctcggt ccggctgact
      721 ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac gttgaaaagc
      781 tcgacgcgca agggattatg gcccgctggc cagaaatacg cgtcccggac aactacatcg
      841 gtttatttga aactgattcc ggttttttgc gcagcgaact ggcgattaaa acctggatcc
      901 aactggcgaa ggaagcgggc tgtgcgcagc tgttcaactg cccggtcacc gcaattcgtc
      961 atgacgatga tggcgtaact attgaaacgg ctgacggtga gtatcaggcg aaaaaagcga
     1021 ttgtctgcgc gggaacatgg gtaaaagacc tactcccgga gctgcctgtc cagcccgtac
     1081 gcaaagtatt tgcctggtat caggccgatg gccgctatag cgtgaagaat aaattcccgg
     1141 cttttaccgg tgaactgccc aatggcgatc aatattatgg ttttccggca gaaaacgacg
     1201 cgttgaagat tggcaaacat aacggaggcc aggttatcca ttcagcggat gaacgtgttc
     1261 cgtttgcgga agtggccagt gatgggtcag aagccttccc gttcttgcgc aatgtattgc
     1321 cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta tgataattcg cctgacgaag
     1381 attttattat cgatactcta cccgcccacg ataatacact gctcattacc ggcctgagtg
     1441 ggcacggttt taaatttgcg tcagttttag gggaaatggc tgccgatttt gcgcaagaca
     1501 aaaaaagcga ttttgatttg acgccattca ggctttcccg cttccaataa tcatcattac
     1561 ggcctccaga atgaggccgt ttttacagga gtatttaatg cgtcggttgg tgaactatct
     1621 cattaataat attcgcgagc atctgatgct ttatcttttt ctctggggat tgttggcaat
     1681 tatggatctg aactatgtgt tttattttta gttttccgca ctgacataaa ctgacaaata
     1741 tttaatctcc ctttcattta gcgtgaaaaa ttgaatgaga ttattcctgc ttctacagca
     1801 ctcaccagac atctctgatg ctgaacccac taaacttatg aagattcttt cattattaat
     1861 tcaaaattat tgaaataaaa ataaatatca ttcaatttgt tatttttgtg ttgcaattca
     1921 ttctccgaca cgccattttc tttctctcgc aaattcgttt tggatgcact tatgtcattc
     1981 acaaataccc ctgaacgcta tggtgttata tctgcggcct tccattggct aagcgcaatt
     2041 attgtctacg gcatgtttgc tttaggtctg tggatggtca cgctcagtta ttacgatggc
     2101 tggtatcaca aagcacccga actgcataaa agcatcggta ttttgttaat gatggggctg
     2161 gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg gtccgctgcc gagttattcg
     2221 cccatgactc gtcttgccgc aaaagctgga catctcgcat tgtacctatt gctgttcgcc
     2281 atcggcatca gcggctatct gatctcaact gccgatggta aaccgatcag cgtttttggc
     2341 tggtttgacg tccccgcgac cctcgccgac gctggcacac aggctgactt tgccggtgcc
     2401 ctgcattttt ggctcgcgtg gagcgtcgtc gtactgtccg ttatgcacgg atttatggct
     2461 ctgaagcatc atttcatcga taaatacgac actctgaagc gcatgctggg aaagtcgtca
     2521 tctgactatg gagtataaaa aatgaaaaaa agcctgcttg gtttaacctt cgcttccttg
     2581 atgttctctg ccggttcagc ggttgccgcc gattacaaaa ttgacaaaga aggtcagcac
     2641 gcctttgtta acttccgcat ccagcatctt ggctatagct ggttatatgg cacctttaaa
     2701 gatttcgacg gtacttttac ctttgatgaa aaaaatccag ctgccgataa agtgaatgtg
     2761 acaattaaca ccaccagcgt cgatactaat cacgccgaac gcgataaaca tcttcgcagt
     2821 gcggatttcc tcaataccgc aaaatatcca caggcaacat tcacctccac cagcgtgaag
     2881 aaagacggtg acgaactgga tattaccggc gatctgacgc tgaatggtgt aaccaagccc
     2941 gtcacactgg aagcgaaatt aattggtcag ggtgacgacc cgtggggcgg taaacgtgca
     3001 ggctttgagg ccgaaggcaa aattaagctc aaagacttca atatcaagac agatttaggt
     3061 ccagcgtctc aggaagtgga tctgattatt tcagtggaag gcgtacagca gaagtaagag
     3121 tgaaatgttg ccggatggca cgcatccggc aatgatattt attctgttgg atcaggaatg
     3181 cccagtgttg tattcagacg tccacgagac ttattaaaga tcttattgcc attttcacgt
     3241 cctgcccgac ggcgtcgctg ttcatctggc ggtaacgcgc tttcttcgca gcaaatctca
     3301 ctacaacaac ctttgtattt ttccgcacat actggacact gaataaacag cagatggcag
     3361 ccatcatttt tacagttggt atggctgtcg cacggcgcac cgcactggtg gcagtgcgcg
     3421 ataatctcat cagatatacg ttcgcccatc cgctcgtcaa aaacaaaatt tttgccaata
     3481 aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt attcaataat tccgccctcg
     3541 atatgccaga ctttgttgaa tccgttatgt ttcatccagg cactggcctt ttcacaacga
     3601 atgccgccgg tgcagtacat gacaattttt ttatctttat gtgcctgcat catctcaact
     3661 gcttttggca gctgctcacg gaaggtatct gccggtattt ccagcgcgtt ttcaaagtgc
     3721 cccacttcat actcatagtg gttacgcatg tcgataaaca atgcatcggg atcgtcaagc
     3781 atggcgttca cttccgccgc ttgcagatac tcaccaacat tgctggcatc aaagtgagga
     3841 tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca tacgcagtac ccagaaggat
     3901 ttcccgtcat catccaacgc gatgttcagg cgtaaaccct ctaaagccgg gtcgaaggca
     3961 tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta cgctgatttg cgcgttaatg
     4021 ccctcatgcg ccagatacac tcgcccaaaa acattcagcg cggtaaacag ttgatataaa
     4081 gcgtcacggg tcaccttagg atcggcaatg tggaaatact tataaaacga aatggtggtt
     4141 cgcggttcgc tctcagccaa cattttggct tttaacgcgt cgttggaaat gcggttgtgt
     4201 aacactggca tggtgtacgg ttcctgcgag atgggaaagt aaaaatccgc ggcatgatat
     4261 agcaattatc gataattaac atccacacat tttacgctac attttagcat taaaaattat
     4321 ttgttattta caagcgcggc aatttcgccc agtcttcagt cacaaatttt ggttgcggac
     4381 gaaaaaatgc gacaatacat acaattgccc gtataggttg aaaaacagga ttgatatgac
     4441 gaatctaccc aagttctcca ccgcactgct tcatccgcgt tattggttaa cctggttggg
     4501 tattggcgta ctttggttgg tcgtgcaatt gccctacccg gttatctacc gcctcggttg
     4561 tggattagga aaactggcgt tacgttttat gaaacgacgc gcaaaaattg tgcatcgcaa
     4621 cctggaactg tgcttcccgg aaatgagcga acaagaacgc cgtaaaatgg tggtgaagaa
     4681 tttcgaatcc gttggcatgg gcctgatgga aaccggcatg gcgtggttct ggccggaccg
     4741 ccgaatcgcc cgctggacgg aagtgatcgg catggaacac attcgtgacg tgcaggcgca
     4801 aaaacgcggc atcctgttag ttggcatcca ttttctgaca ctggagctgg gtgcgcggca
     4861 gtttggtatg caggaaccgg ggattggcgt ttaccgcccg aacgataatc cactgattga
     4921 ctggctacaa acctggggcc gtttgcgctc aaataaatcg atgctcgacc gcaaagattt
     4981 aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc tggtacgcac cggatcatga
     5041 ttacggcccg cgctcaagcg ttttcgtccc gttgtttgcc gttgagcagg ctgcgaccac
     5101 gaccggaacc tggatgctgg cacggatgtc cggcgcatgt ctggtgccct tcgttccacg
     5161 ccgtaagcca gatggcaaag ggtatcagtt gattatactg ccgccagagt gttctccgcc
     5221 gctggatgat gccgaaacta ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat
     5281 gatggcacca gagcagtata tgtggttaca ccgtcgcttt aaaacacgcc cggaaggcgt
     5341 tccttcacgc tattaaatct tccatgctgg atgcttcaga atggcatccg gcattaccac
     5401 agaaaatccc cctgatttag cgataaaagc tctctggatt gcgccccctg gaagtcgggc
     5461 gcataattag tgtgcttatc ttttcttctt atgttcaccg cgcctggcgc accaacagcg
     5521 gattgctatg tcaccctgtg aaaatgacac ccctataaac tggaaacgaa acctgatcgt
     5581 cgcctggcta ggctgttttc ttaccggtgc cgccttcagc ctggtaatgc ccttcttacc
     5641 cctctacgtt gagcagcttg gcgttaccgg ccactccgcc ctgaatatgt ggtccggtat
     5701 tgtcttcagc attacatttt tattttcggc catcgcctca ccgttttggg gtggactcgc
     5761 cgaccgtaaa ggccgaaaac tcatgctatt acgctctgct ctcggcatgg gcatcgtgat
     5821 ggtgttgatg ggactggcac aaaatatctg gcagtttttg atcctgcggg cgcttcttgg
     5881 tttacttggc ggatttgtcc ccaacgctaa tgctcttatc gccacacaag taccgcgtaa
     5941 taaaagcggc tgggcgctgg gtacgctctc cacaggcggc gttagcggcg cgttgctcgg
     6001 cccaatggct ggcggtttgc tcgccgatag ctacggctta cgtccggtat tctttattac
     6061 cgccagtgtg ctcatactct gctttttcgt caccctgttt tgcatcagag aaaaattcca
     6121 gccggtcagc aaaaaagaga tgctgcatat gcgggaagtg gtgacatcac ttaaaaaccc
     6181 gaaactggta ctcagcctgt ttgtcaccac gttaatcatc caggtggcga cgggctcaat
     6241 tgcccccatt ctgacgctgt atgtccgcga actggcgggt aacgtcagta acgtcgcttt
     6301 tatcagtggc atgatcgcct cggtgccagg cgtggcggct ctgctaagtg caccacgact
     6361 cggcaaactt ggcgatcgaa tcggccccga aaagatcctg attacggcgc tgatcttttc
     6421 tgtactgctg ttgatcccaa tgtcttacgt tcagacgcca ttacaacttg ggattttacg
     6481 ttttttgctc ggtgccgccg atggtgcatt actccccgcc gtacagacac tgttggttta
     6541 caactcgagc aaccagatcg ccgggcgtat cttcagctat aaccaatcgt ttcgtgatat
     6601 tggcaacgtt accggaccat tgatgggagc agcgatttca gcgaactacg gtttcagggc
     6661 ggtatttctc gtcaccgctg gcgtagtgtt attcaacgca gtctattcat ggaacagtct
     6721 acgtcgtcgt cgaatccccc agatatcgaa ctgatttttc gcctttcata cttgcaaaag
     6781 cggagaatca gctatccttt tccctgaaac ctcatcaact caaagggaga atcgtgatga
     6841 ccatgtacgc aacgcttgaa gaagccattg acgctgcccg cgaagaattt cttgcagaca
     6901 accccggcat cgacgccgaa aacgcgaatg tgcaacagtt caatgcccaa aaatacgttt
     6961 tgcaggacgg cgacatcatg tggcaagttg agttttttgc cgacgaaggg gaagaaggtg
     7021 aatgtttacc catgcttagc ggtgaagctg cgcaaagtgt ctttgacggc gactatgatg
     7081 agatagagat ccgccaggag tggcaagatg agaatacatt acatgaatgg gacgaggggg
     7141 aatttcagct tgagccaccg ctggataccg aggaaggacg cactgcagct gatgagtggg
     7201 atgaacgtta atcactcata cgggccatga tgtacgtcga tcggcagcag taaagtatcg
     7261 aaaaccaggg agaatggcag atcaaggatc gtgacgtaac gccaggcgga gtcacgcacg
     7321 tcccattgca caccgggata atattggttg ccatgcccct gccccggtat agtgcgacta
     7381 atgatgctgc cacagccact cagcaaggtc accattatgc tcatcacaat taaacgcatt
     7441 tgatacctct tccgtaaaaa ataccggcat aacgccggta tttttattgg ctcgttgttt
     7501 ttgccggatg cggcgtgaac gctttacccg acctacaaaa tcttgcaaaa tcaataaatt
     7561 gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc aactacgttt ttattgcgaa
     7621 gccgcatccg gtttacgcaa tgccagtgga ttgagcttta tcccttcgta ataactcacc
     7681 catgaagaat atctctccgg ggaattccag acgcggaaat gcagacgggc catcgtcacc
     7741 ggatcgctta gcagcaccag gcgacgatcg cgattcagct tctctggcgt ctcgttcagc
     7801 gcctgttcaa cgtggcggtc acgggcgatt tccagcacct tgctggcgcg gtgacgcgcg
     7861 gtcgccattg cggttgccag agcgttaaat gacgggttaa acaccgcgtg cataaaacca
     7921 tcatcaaggg agcgttgacg attcatctca aggaaccgat cggtatcaac cagcacctgc
     7981 ggcggtgaat actcttccgg gatcaggaac agtttccagc gtttggttcg cagaccaacg
     8041 gtggcacggc tggaaataac cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt
     8101 gccagccaga acagaaaacg cagatccagc cacgccatcc caacagccca cactaaccct
     8161 aacagcagct gtgagccgtg gcgtttgaac gcttcccccc aggaagtgga gtcatcatca
     8221 cgctgcggcg aattccacac tacttcccag ccaaggaacg cgctgacgac gaagaccgta
     8281 tggaacaaca tgcgaaccgg agccagcagc acggaaaaca gcacttccag cagcaacgat
     8341 aatgtaacgc gccagaagcc gccgtattct ttcgttcctt tgcaccagat aagcaaaatg
     8401 ctcaatagct tcggcaggaa caacagcacc atggtcgaag caaaaagtgc aatcgccagc
     8461 tcaggacgcc actgcggcca taccgggaac aactgccgtg gttgcaggaa gtattgcggt
     8521 tcggtcaacg catgtactac ctgcaatgca gtagagagcg cgaggaacat aaaccacagc
     8581 ggagcggaga gataagacat cacgcccgtc aggaacaccg cacggtgaac cgggtgcata
     8641 cccttcacca ggaacagacg gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt
     8701 tttagctcat caagcaagtt aggcggtaat tcttcataag aacccgggag atcgtaagca
     8761 atccagaccc cccaacctgc acggcgcatc aacgccgctt ccacgaagtc atgtgacagg
     8821 attgaaccgg caaaagaacc ttcgcccggc agcggagcca gtgcacagtg ctcgataaac
     8881 ggtttcacgc ggataatcgc gttatgcccc cagtagtgcg actcgccaag ttgccagaag
     8941 tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg cgaactgctg acagcgcgca
     9001 tacagcgtat ccatgccgga cgctttcggc gacgactgaa tgatcccggc gttcgggttg
     9061 gcttccatca ggcgcaccag gccgcacaaa caatcaccgg tcattaccga gtcagcatcc
     9121 agcaccacca tgtagctgta ctggctgccc cagcgacggc agaagtcatc gatattaccg
     9181 cttttacgct tcacgcgacg gcggcggcgg cgatagaaaa tctgaccttc gccaccgact
     9241 tcagcgataa gctccatcca ggctttttgc tctgcgacgc agatatccgg gttataactg
     9301 tcactaagaa tgtagacatc aaaatgtttg gcattcccgg tggcttttac tgattcccac
     9361 gttgcacgca agccagcaaa aacacggttc acgtcttcgt tacagatagg catgatcaac
     9421 gccgtgcgat gctccgggtt taatggttca tcgccaactg ttgacgcaga tatactgtat
     9481 ttatcgcgac caataagcag ttgcaggaag cccatcaacg ccgtccagaa tccggcggac
     9541 acccaacaga acagtaccgc aaagaggatc aggataccgg tttgcagcat ataaggcaga
     9601 agctgcataa aggaaaccca cacatcctga ccaaccatat ccataggatt aatcagcgcc
     9661 cacccctgat aaggaagaat ggtcttcata taccaggtcg cgacaacagt ttgcgcgagc
     9721 gtcaggatca acagaatgta acggcggatg gtaccgacgg tacgccactt ttgctcactc
     9781 tcctgctctt ctttggtcaa acgagccaga tagcgcggcg tcacatcgcg tccacgcagg
     9841 cgatcccaga aacggcctac cgggttggta cgccacgggt cgggaaacat cgaggagcgt
     9901 tttacttctg gcatcgcctt tagctgatcg cgcccttcgt cgtctttaat taactgtcca
     9961 tcagcaagtg aatctggcca ggcttgttcc agacgcgcct ttaccgagcc ttgcggggag
    10021 tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg cctgatgaac ggcgcggata
    10081 tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg gcattgcgtc aatgtactca
    10141 gttgtcttat tcattggcag gtaactggta gctccaggtt tcactcaacg tctgatcggc
    10201 attcaccagc gcagcacgca tttcagtggt tttcttggca tctttcactt tcacacgcat
    10261 caccagacgc cagcctttgg taaccgggtt ataacgcacc gtgctttcaa ctatctcacc
    10321 attatcacca atgctggttt gcgctgtgac cggggtatcc tctggcagtt ttttcatctc
    10381 agcgccggta aaatcgacca caaaggcgat agtaccgtca ggctggcgaa tcaggttcga
    10441 ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg
    10501 cagtttgtct tcatcacggc tgaaggtgat ggtgtattta aagttcatct ctttacccgg
    10561 ctccggcagc tgatccggcg tccagtaagc gacgatgtta tcgttggttt catcgttggt
    10621 tggaatttcc accagctcaa cgctgccttt gccccactcc cctttcggag tcacccatgc
    10681 gcttggacga agatcgtaac gatcatcgag atcttcaaag cgggagaaat cacgaccacg
    10741 ctgcaataga ccgaagcctt gcgggttttc catcgagaag ctgctgaccg ctaaatgttt
    10801 cgggttattc aacggacgcc agatccattc gccattacca gcatggatag acagaccgtt
    10861 agagtcgtgc aactccggac gatagttatt tgcaggcgac ggttggttcg gcccaaacag
    10921 gaacatactg gttaacggtg caacccccag tttgccgact ttatcgcgca gatagatttt
    10981 cgactgcaca tccacaaccg tgtcacgtcc tggcataact acgaatttgt aagcacctgt
    11041 cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt ttatcagtcg gttttggacg
    11101 ctcgatccag aactctttga agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat
    11161 tgccaggccg cgtgcagaaa ggccataaac ctgacctgca ccaatcacgc ggaaatagct
    11221 ggctccgagc atgctgacga tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac
    11281 tttgaaaccg gcaaaaccaa ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa
    11341 agtgaaataa tccgggctgt atttgattcg tttgactgcg gtggcagtca cttcatttat
    11401 tttgaccggg gtatcgaagt acataccctg atggtagaac tcgagtttga atggggtctt
    11461 cagattgttc cagtacgctt tgtcatgatt aaactggatc tgctgatagt ccgcgtattt
    11521 catatcgcgg aaaacggagg gcaagttgct tttgggcgcc tcatagcctt tcccggctaa
    11581 ggattgagct tgctttgcga catcatcaat actgaaagcc cagcttgaag atgtatacag
    11641 ggttaacatt actgcagcac tcaaccaacg cattttcatc atttgtagtt tatgtttcat
    11701 aattagtaag cacttccccc tttgtgtgct tatatcgatc cgatctattt taatggataa
    11761 cctgccattc cgacaacaga atcccggtat cgttcagcac actggctcat ggattaagca
    11821 cctgattgca accgttttca ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt
    11881 gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca gacgcttcca ttctggttag
    11941 tggtagcgat gatgctatgc ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg
    12001 tgtcgctatg gcaacaagcc taaaggcata acccgacagg gataagacga aaaatccgag
    12061 attagttaac catatatgaa tccagtaccc gcgcaacgtg aatatttcct cgactccatc
    12121 cgcgcctggc tgatgttgtt agggatccct tttcatattt ctttaatcta ttcgagccat
    12181 acatggcatg tgaatagcgc cgaaccgtca ttgtggctga ccctttttaa tgacttcatc
    12241 cactcgttcc gcatgcaggt gtttttcgtt atatccgggt acttttccta catgcttttt
    12301 ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg aacgtgtagg gatcccgatg
    12361 ttaacagcca tccccttact gacattgccg caatttatta tgctgcaata cgtcaaaggg
    12421 aaagcggaaa gttggcctag gctgtcattg tatgacaaat ataatacgtt ggcctgggaa
    12481 ttaatatcac acctgtggtt tttactggtg ttagtggtca tgacgacgct gtgcgtatgg
    12541 atatttaaac gcatcagaaa taatttagaa aattctgata aaacgaataa aaaattctcg
    12601 atggtaaaat tatcggtgat ttttttgtgc ctcggcatcg gttatgcggt aataagaaga
    12661 acgattttta ttgtgtatcc acccattctg agtaacggca tgttcaattt tattgtcatg
    12721 caaacgttat tttatttgcc gttctttatc ctcggcgcac tggctttcat tttccctcat
    12781 cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt
    12841 gtcgcttatt tactcaacca gcgctatggc agtggcgatg cctggatgta tgaaaccgag
    12901 tcggtgatca ctatggtcct cggtctgtgg atggtgaatg tggtcttctc ctttggccac
    12961 cgtttgctta acttccagtc agcgcgggtg acttattttg ttaacgcatc gctgtttatc
    13021 tatctggttc accacccgtt aacgctgttt ttcggcgcat acattacacc acacatcacc
    13081 tccaactggc ttggttttct ctgtggcctg atattcgtag tagggattgc gataattctg
    13141 tatgaaattc atttacgcat cccattattg aagtttttgt tctctggtaa accggttgtt
    13201 aagcgtgaga acgataaagc accagcccgt taagtcgcgt ttacaataac cattccacgg
    13261 gcaatatcga cgccagtttg accataaccc gcttccagaa actggtggcg ggttcttttt
    13321 tgagaataat ctctttcttt gcatgacgat caacccagtt gatccatccc cacctgtcca
    13381 gacggagctg ccaggccgca tcatactggc tctgaataaa gcgtttatca attaactgtg
    13441 ccagcgtttc gctctctatc acgaagccca tttcagtatt gagcaacgtt gaacgcggat
    13501 cgaaattgaa agagccgata aacaccgttt taccatcgat actaaaggtt ttagcatgca
    13561 ggctggcccc tgaattaccg gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg
    13621 tcggcttgag ttcatataat tccaccccat agcggagcaa ttttttgcgc cagcgcgcgt
    13681 atcctgcatg gacgacagca acatcgttag cggcaagaga attggttagg atcgcaatct
    13741 ttaccccttt tctcaccatc cgtaagagtt gcgccacacc tgcacgtgtc ggtacaaaat
    13801 aggaagagat aatatcgatg cgttcactgg gtgagcccat gatatcgaac aggcgctgcg
    13861 gtaacagtga atgccgtttt gccttgccct cccctttcgc cggatcatca cttaataaac
    13921 gtgttttcgc ccagataagc gacaacgttc catcaaccag atgatgtata aacggactgg
    13981 attccatttt gcgtaaataa cgatgcgtca tggcatcgtc atgccaggat gccggtaact
    14041 cgatgcgatc cgccatttca ccttccggga catccagcac ctgctgtaag ggtgaaaccg
    14101 atttgcaata ccaatagcgg gcgaaatcat cggcaacgtc ctctaccacg ggtcctattg
    14161 ccatgacatc taaatccgaa aaaagtggct cttcccctgc accaaaatag gcatcaccaa
    14221 tatttcgtcc tcccaccagc gtcaccacgc catcgacagt gaaactttta ttgtgcatac
    14281 ggcgattgag acgggaaaag tcggtgatat aaccaagcgg acgtagcaag cgaaacgaga
    14341 aaggattaaa aagccggact tcaatgcgtg gatggctgtc aagcaagcgt aaaatgttat
    14401 caagtccggg cgtattgttg tcgtccagca acaaacgtac gcgaacgcca cgctttgctg
    14461 cggctaacag ggcggaaaac agtaaccgac ccgacatatc gtcctgccag atgtaatact
    14521 gaacatccag tgtatgctct gccatttcag ccagacgata ccgggcggca aacgcatcca
    14581 gacttttctc cagaggaaaa agtccacact gaccgggatg ttgcgaacac agtggcagca
    14641 ccgcgctcgc cagctgaggc aaatcattca tctccttgtt gggtaaggag tctttcgtag
    14701 aggtgggcgt tttcttcatc ataacagaca aagtatacct gttcgggtaa agcgtgacgg
    14761 gtaataaatt ctgaaacggt ttttactgcg atttcagccg ctgccgcacg ggggtaacca
    14821 taaaccccag tactgattgc aggaaaagcc actgacgtat agctgtttgc tgccaccagt
    14881 cgcaggctat tgagataggc atcctgcaaa agctggtctt cgttttgttc accaccacgc
    14941 cagactggcc cgacggtgtg cactacggct ttcgcgggaa gatcgcctgc aagcgtaata
    15001 acggcatgcc ccgtagggca atcgccctgc tgctgcctga cttttaaaca ggcatccagc
    15061 agggccggac ccgctgcgcg atgaatggcc ccatcgacgc cgccgcctcc cattaatgac
    15121 ggattagccg cattcacaat cacatcaacg gccagtttgg taatatcacc ctgcacaaca
    15181 tgaatacgcg ttttcataac gcctccttac accttcttgt ttcttaagtg tacagcaggc
    15241 tttttggtta aatcgcattt ttctgctcag caacgtgagt tgttcagaaa cttagttata
    15301 ttcgatgttc attacagcca gctttttctg tttatcaaga tactgcactt ttactgaggc
    15361 tatttgcgcg tttttaacat tgccgctcat taacgctttg ctggaatagg ttttacgcgg
    15421 aacagaacca ttctgtgggc agttcatttc tacggctgat gactgggtgc tgacgtcaca
    15481 tgccggttcc acaatttggc cataaaaatg gatcacgccc ccttgcgcgc tgcttcctgc
    15541 aatggcaata aaaggaaaaa acaaactgcc gagcataaga gagtttaaaa aaggtctgaa
    15601 catttattct atttcctcaa tgattaatca tccttgaggg ttgtgttatc catactttca
    15661 ggttggctga ttcgcagcag accattctct ccaggttcat cttatgctcg atatttcaac
    15721 aaattaagac ttttctgaag agggcggcca ttgttgtgat aaatgaagtg actgtccatc
    15781 agaaacagta acaactattt tcacccgatc gtccggggga atatttaaac tcaacttcgt
    15841 caaagcaatg agttgattag caggcaatga aagggtcttt tcttgcttcg tctgactttg
    15901 ccctgaactg ccttcgcgca gggacaatat ttgtactctg cacagacaag attgagtaag
    15961 agtgacttca ggaataatgg tgtacatatc cccttgctgg gtcgtattaa aggttatctg
    16021 actggaaagt gccgcaagga gtaataacgc attcatattc ttctcccgaa aaaaaacagg
    16081 gcttgcgccc tgtttctttc atactgatga tgtattagta ctgatgagcg gtcgcgttgt
    16141 taccaaagcc aacctgagtg acgttaacgc tggagttaga tgcagtctgg tcaacagcag
    16201 caccgttacc gccaccgaac tgtttaacag tcatagtaga atctttacca ttccactgat
    16261 caagagtagc gctgttgccg aaaccacgct gagtcagatc gattgagctg tcatcagaac
    16321 cttggcccac atctgcgcca ttaccgccgc cgtgctgggt aatggtcaga tcagagttac
    16381 gggcgtcagc ttgcagagca agagcagagt taccgccacc gtactggtaa atattcagct
    16441 ctgaattcgg gccgctgtta ttaccgccac caccgtggtt gccaccgccg ccgccgtact
    16501 gaggaacaac acctgccaga gcgctaccag agaatacgat tgctgcaatt gctgctactt
    16561 ttaaaagttt catgtaaaac ccccatcgga ttgatttaaa agtcgaatgg aaattaacgt
    16621 tgtgtcacgc gaatagccat ttgcgactgt ctctgcacta caattgccgt tttttgagta
    16681 ccatactgtg taatatttgc tttattacca gaacctttct ggataatcat cgcagtatta
    16741 ccataagcac cttgcgaaat actggcatcg ttggcactgc ccgcctgatc aatatatgca
    16801 aggttataat ctcctgtctg atcaatcttt gcccggttgc tactaccttc ttgcgcaaca
    16861 accgccaaaa gttttgagcc tccctgccgt aactgagcac tattattagt cccagcttga
    16921 ccaattatgg ctgcctgatt aaatgaagac ttactcaatt catttaccgc aaagttatat
    16981 tctgaattag ctaaatcata acctgctgcg gctgcaatcc caggcgcacc cagtattgtt
    17041 aacatcataa ataa
//
LOCUS       DAZFRG010000099        16985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFRG010000099 DAZFRG010000000
VERSION     DAZFRG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.099"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..1252)
                     /gene="punC"
                     /locus_tag="ACHKUC_002539"
     CDS             complement(41..1252)
                     /gene="punC"
                     /locus_tag="ACHKUC_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO6618783.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            1365..2297
                     /gene="punR"
                     /locus_tag="ACHKUC_002540"
     CDS             1365..2297
                     /gene="punR"
                     /locus_tag="ACHKUC_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO6618784.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYSF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(2294..3319)
                     /gene="purR"
                     /locus_tag="ACHKUC_002541"
     CDS             complement(2294..3319)
                     /gene="purR"
                     /locus_tag="ACHKUC_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO6618785.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            3618..3707
                     /gene="cydH"
                     /locus_tag="ACHKUC_002542"
                     /gene_synonym="ynhF"
     CDS             3618..3707
                     /gene="cydH"
                     /locus_tag="ACHKUC_002542"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO6618786.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3873..5042
                     /gene="ydhP"
                     /locus_tag="ACHKUC_002543"
     CDS             3873..5042
                     /gene="ydhP"
                     /locus_tag="ACHKUC_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6618787.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLVIPFLARNEFGAAMSMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSVRKQPETVCVANS"
     gene            complement(5188..5769)
                     /gene="sodB"
                     /locus_tag="ACHKUC_002544"
     CDS             complement(5188..5769)
                     /gene="sodB"
                     /locus_tag="ACHKUC_002544"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO6618788.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(5897..6712)
                     /gene="mepH"
                     /locus_tag="ACHKUC_002545"
     CDS             complement(5897..6712)
                     /gene="mepH"
                     /locus_tag="ACHKUC_002545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO6618789.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     NFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            7047..7394
                     /gene="grxD"
                     /locus_tag="ACHKUC_002546"
     CDS             7047..7394
                     /gene="grxD"
                     /locus_tag="ACHKUC_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO6618790.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(7563..7673)
                     /locus_tag="ACHKUC_002547"
                     /pseudo
     CDS             complement(7563..7673)
                     /locus_tag="ACHKUC_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(7766..8413)
                     /gene="rnt"
                     /locus_tag="ACHKUC_002548"
     CDS             complement(7766..8413)
                     /gene="rnt"
                     /locus_tag="ACHKUC_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO6618791.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            complement(8516..8923)
                     /gene="gloA"
                     /locus_tag="ACHKUC_002549"
     CDS             complement(8516..8923)
                     /gene="gloA"
                     /locus_tag="ACHKUC_002549"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO6618792.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(9004..10101)
                     /gene="nemA"
                     /locus_tag="ACHKUC_002550"
     CDS             complement(9004..10101)
                     /gene="nemA"
                     /locus_tag="ACHKUC_002550"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO6618793.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(10138..10737)
                     /gene="nemR"
                     /locus_tag="ACHKUC_002551"
     CDS             complement(10138..10737)
                     /gene="nemR"
                     /locus_tag="ACHKUC_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO6618794.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            10840..11079
                     /gene="ydhL"
                     /locus_tag="ACHKUC_002552"
     CDS             10840..11079
                     /gene="ydhL"
                     /locus_tag="ACHKUC_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO6618795.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKSAPSDEPEQPSLF"
     gene            11128..12024
                     /gene="ydhF"
                     /locus_tag="ACHKUC_002553"
     CDS             11128..12024
                     /gene="ydhF"
                     /locus_tag="ACHKUC_002553"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO6618796.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            12105..12626
                     /gene="sodC"
                     /locus_tag="ACHKUC_002554"
     CDS             12105..12626
                     /gene="sodC"
                     /locus_tag="ACHKUC_002554"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6618797.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(12635..14647)
                     /gene="ydhK"
                     /locus_tag="ACHKUC_002555"
     CDS             complement(12635..14647)
                     /gene="ydhK"
                     /locus_tag="ACHKUC_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6618798.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMSKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(14647..15504)
                     /gene="ydhJ"
                     /locus_tag="ACHKUC_002556"
     CDS             complement(14647..15504)
                     /gene="ydhJ"
                     /locus_tag="ACHKUC_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6618799.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNFYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSGLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(15507..15743)
                     /gene="ydhI"
                     /locus_tag="ACHKUC_002557"
     CDS             complement(15507..15743)
                     /gene="ydhI"
                     /locus_tag="ACHKUC_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO6618800.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            15944..16378
                     /gene="slyA"
                     /locus_tag="ACHKUC_002558"
     CDS             15944..16378
                     /gene="slyA"
                     /locus_tag="ACHKUC_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO6618801.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(16425..16892)
                     /gene="slyB"
                     /locus_tag="ACHKUC_002559"
     CDS             complement(16425..16892)
                     /gene="slyB"
                     /locus_tag="ACHKUC_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO6618802.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
BASE COUNT         4230 a         4197 c         4435 g         4123 t
ORIGIN      
        1 aattttgtta gcagcctaag tataagtata tcgatataga tcagtgtgat tcgctatgag
       61 cgacttcggc attgccatga ttctggcagc caacttcttc acaacgttgc atcatgtaac
      121 ccagagcgac cagcactact gttgataaca tcacgctggt ggtggtgagc aatggcgtac
      181 tgatactgat aagccaggaa actaccagac ttgcgaggaa gcacagaccc agttgcagag
      241 tgttctgcaa cgctgcggcg cgaccggttg catgtgggaa gggacgcagc gcctgggcaa
      301 caacaatcgg gtagatcgcg ccgttggcaa tcgccatcac acagaatggg atcaggattt
      361 cgaccagcga cacatggcta atgaagcccg cagcccaggt cgcaatgacg ctgacagcaa
      421 acagtaccag caaccacggt aataactgct tgccttgcca tttctgcagc gcggcgcgac
      481 agccataacc accaatcaga aacgcgatag tttgcgggac gtaacttaaa ccaataactg
      541 ccggactgta acccatttca ctaaggatga acggtgaacc ggtcagccat gcaaaaaaac
      601 tggctgaaca ggccgcgtat atcagcacgt tgccgcgata ggttttagaa cgtagcaggt
      661 cggtaaaggt cagaccatcc tgactattgt tacgggcctt cgtcgtgggt ttgagccaga
      721 aaataggcag aatcagcacc acggtaatgg caaacagggt ggcgaaaatc gcctgccagg
      781 aaaaatggac cagcagccag cttcctaaca gaggagccag tgccggagac agacccacca
      841 gcggcatgat ggtcgcaaaa atacggttaa ctttctgtga aggataataa tctgtcacta
      901 atgcttgcca gataaccgcc gcggcgcaga cacccacagc ctgtacaaaa cgcaatatca
      961 gcagcgtggc ggcgttttct acccacagca tccccagact acctaacgca aaaattgtca
     1021 ggccgatgaa taacaccggt ttacgaccgt aacggtcgga gagtggcccc cacagaagct
     1081 gggctgcggc aaaaccggca aggaacagac taaggctggc actgacagca gacgcaggcg
     1141 tttgcaggtc ggcttgtatg gcggcgaaag caggcagata catatcggtt gccagaaaac
     1201 cgagtacgct caaacctgcc agccagacta aaaatctttt cccaggttgc atgtatattc
     1261 tctaaatctc tcaattaaac accgccgcaa agtgtaggga gtgaaattag tgttgtgaaa
     1321 cggtaatatt tgatgattgg tttcaaaaat tttgcaggca gactatgtgg tcagaatatt
     1381 ctctggaagt tgttgatgcg gtagcgcgta atggtagttt tagcgccgcg gcccaggagt
     1441 tgcatcgcgt tccttctgcg gtcagctata ccgtgcgtca gctggaagag tggctggcgg
     1501 tgccgctttt tgaacgacgt caccgtgatg tggaactgac cgctgccggg gcgtggtttc
     1561 tcaaagaagg acgctctgtt gtcaaaaaaa tgcagatcac ccgccagcaa tgtcagcaga
     1621 tagcgaacgg ctggcgcggt cagttagcta tcgcagtgga taatattgtc aggccagagc
     1681 gaacacggca gatgatcgtt gatttttatc gccattttga tgatgtcgaa cttcttgtct
     1741 ttcaggaagt gttcaacggt gtctgggatg cactttccga cgggcgcgtg gaactggcga
     1801 ttggcgcaac acgcgcgatt ccggtaggcg gtcgttattc cttccgggat atggggatgt
     1861 taagctggag ttgcgttgtt gccagccacc acccgctggc gttgatggat ggcccgttca
     1921 gcgatgatac gttgcgcaac tggccgtcat tggtgcgcga agacacctcg cgaacgctac
     1981 ctaaacgtat tacctggttg ctggataacc aaaaaagact tgtcgtgcct gactgggaat
     2041 catcggcaac ctgtatctcg gcaggattat gcatagggat ggtgccaaca catttcgcca
     2101 aaccgtggct taatgagggg aagtgggtag cactagaact ggaaaacccc ttcccggatt
     2161 cggcatgttg tctgacatgg cagcaaaatg atatgtcgcc tgcgttaacc tggttgcttg
     2221 agtatctcgg cgatagtgag acgctgaata aggagtggct gcgggagccg gaagagactc
     2281 ccgcaacggg tgattaacga cgatagtcgc ggaacgggcc gtcagccacg gagcggcgtt
     2341 caatcaagcg cggatgcact tcaatggact gcggttcttc gcgtttattg acgatacgat
     2401 ccaacagcat gttgaacgcc gtttcgccca gcgaatcttt tggctgatgg atcgtggtca
     2461 gcgctggcgt aaaatagcgg gcgttgcgca cgttatcata accgatcagc gaaacatcct
     2521 gcggcacgcg cagacccatt tcatcagcag cacaaagtgc gcccattgcc atgatatcgc
     2581 caccacagaa gacggcagta gggcgatgcg gctgcgacag tatttgctgc atggcgcgat
     2641 aaccggattc aggttcaaaa tcaccctgta caatccagct ttccggcacc ttgatcatcg
     2701 cttcttccat cgccttcata aaaccggcaa ggcggcctgc gccggtgtta cgttccagcg
     2761 ggccagggat aacgccgatt tcgcggtgac cgcgttcaat cagataacgc ccggccatgt
     2821 aaccgccttc gaacgcgtta tcaatgaccg catcggtgaa gtcagctttt gcttcacccc
     2881 agtccatcac caccattggg atatggcgat actcttccag catcgccagc aacggctctg
     2941 ggtactcaga acacatcacc agcagaccat cgacgcgttt ttgcgccatc atcgacagat
     3001 aagcccgctg tttctcaaga ttgttccacg cattgcccag aatcagggtg taacctttct
     3061 ggaagcaatt tttttcgacc gcttcaatga tctcggcaaa ataggccgct tcgctgctgg
     3121 tcgccagcaa accgatagac ttggtgtggt taaccttcag gctacgcgcc accgcgctag
     3181 gggagtagtg taattctttg attgctgccc acacggcatt gcgcgtttct tcagcgacga
     3241 aacgtgtttt gttgatcacg tgtgacacag ttgtagtgga aacgtttgct cgtttcgcta
     3301 catcttttat tgttgccatt ccatttcact ccagacccta acttcagctc ctgcaaattc
     3361 gcaaggtaaa cgtttgcctt tacacacctt acgcacaaca aaaatgttgc ggtacgccgg
     3421 aaaaacgaca cggagcgtca ggaaggggtc aatggccagt acgcaaataa tgtaagcgtg
     3481 gaattttggc cgatcttgac gaaaagggga agtagtaaaa acacttatca ttataaatga
     3541 cgggagattt tttcatcaca gtgtgtaaaa atgcgtaata tcactttttg tgtggtaata
     3601 aaaaaggaga aaacttgatg agcaccgacc ttaaattttc actggtaaca acgattatcg
     3661 tcctcggttt gatcgtggcc gtgggtttga ctgccgcgct gcactgattt cctctcgagg
     3721 gagcgccttc tccctcgcat ctccttcctg tttgttagca aaatggcaaa agtgttttct
     3781 ctttttgtta ttttcatttt tttgtgatta ctgtcatgct ttcgccactt taatgaatat
     3841 cgcgtaatga cgctaagagg tggagataag gcatgaaaat taactatccg ttgctggcgc
     3901 tggcgattgg cgcgtttggt atcgggacaa cggagttctc gccaatgggc ttgttgcccg
     3961 tcattgcgcg cggtgtggat gtctcgattc ccgctgccgg aatgttaatc agtgcttatg
     4021 cagttggcgt aatggttggc gcgccgctga tgacgcttct actttctcat cgtgcccgcc
     4081 gcagtgcgtt gattttcctg atggcgattt tcacgcttgg caacgtactt tccgccatcg
     4141 cgccggatta tatgaccctg atgctttcac gcattttgac cagcctgaat cacggggcat
     4201 tttttggttt gggttcagtc gtggccgcaa gcgtagtgcc aaaacataaa caggctagcg
     4261 cagttgccac tatgtttatg gggttaaccc tggcaaatat cggtggcgtg ccggcggcga
     4321 cctggttggg cgaaaccatc ggctggcgga tgtcatttct ggcaacagcg gggctgggcg
     4381 tgatttcaat ggtaagtctg ttcttctcat tacctaaagg tggcgcaggg gcgcgacctg
     4441 aagtgaaaaa agagctggcg gtattaatgc gtccgcaggt gctgtctgca ttgctgacga
     4501 cggtactggg cgctggtgca atgtttaccc tctacaccta tatctctccg gtactgcaaa
     4561 gtattaccca cgtaacgccg gtgttcgtca cggcaatgct ggtgctgatt ggtgtcggat
     4621 tttctatcgg taactatctc ggcggcaaac tggcagatcg ttcagttaac ggcacgttga
     4681 aaggcttttt gttgttgctg atggtgatta tgctggtaat cccgttcctg gcccgcaatg
     4741 agttcggcgc agctatgagc atggtagtgt ggggagctgc aacctttgcg gtcgtaccgc
     4801 cgttacagat gcgcgtgatg cgtgtcgcca gtgaagcgcc aggtctgtct tcatcagtca
     4861 atattggtgc ctttaatctt ggaaatgcgc tgggagcagc tgctggtggt gcggtaattt
     4921 ccgctgggct gggatacagt tttgtgccgg tgatgggggc gattgtcgcg ggactggcat
     4981 tattgctggt gtttatgtca gtcagaaaac aacctgaaac agtttgcgtt gccaacagct
     5041 aattaaacaa aaacgcagaa ggataacctt ctgcgttaca gttgctggca acgtgcgatt
     5101 tatcttgccg aatgcgctgt taacgcctca tccgtgttgg catggcgcgc gaagcgcatc
     5161 aggtaatgtt gcatttgcca tcagttatta tgcagcgaga tttttcgcca cgaattccca
     5221 gttcaccagc gcccagaagt gctccagata gccaggacgt gcattgcgat agtcgatgta
     5281 ataagcgtgt tcccagacat caacggtcag cagcggagtc gcatcggtgg tcagtggagt
     5341 acccgcgtta gaggttgaaa cgatagccag tttgccatcg ctgtttttca ccagccaggt
     5401 ccagccagaa ccaaagtttt tgatcgctgc atcagtaaac tgagctttga aatcggcaaa
     5461 gctgccaaaa gatgcggcga tagcttcagc gacttttcca gtcggttcgc cacccgcgtt
     5521 cggtgccagg cagttccagt agaaagtatg gttccagacc tgagctgcgt tgttgaatac
     5581 gccaccttca gagctgcgaa taatctcttc cagtgattta ccttcaaacg cggtaccttt
     5641 gatcaggttg ttcaggttag tgacataagt ctgatgatgc ttgccgtagt gatactcgat
     5701 ggtttccgca gaaatgtgcg gtgccagagc atctttagca tatggtagtg caggtaattc
     5761 gaatgacatt gctactctcc tttattatta atttgcatac gtacaatagc cttattgtgc
     5821 gtatggatag ggtagcaaat taaaaggtga gacaaaagat gaacttaccc tgttgccgta
     5881 acaacagggt aaagttttag cgaagtgttt ttggggtcat tacccggcga gcgccaacat
     5941 agtggcgctg ccagtagtct tcactgagag aagtgatttg aatttcctga cccgtacgcg
     6001 gtgactgaat aaatttgccg ttgccgacat acacgccgac atgatcggct gtaccgcgtc
     6061 cctgagtacg gaagaagacc aggtcgccgt ttttcagttc actacgttcg attggcgctg
     6121 catcgcgcag gtgatacatt tcattcgccg tacgcggaat acgaattttc accaaatctt
     6181 tataagcgta ataaaccaga ccgctgcaat caaaaccggt acgcggtgaa ctgccacccc
     6241 aacgatatgg cttaccaatt tgctgcatca gtttattcat tgccactttc gtggcttttt
     6301 gcaccttcgc tttgtgcgcg tcggcaaggg ttcctgacgc ggcatttttg actttcacac
     6361 aatgcgattt atagccctta cgtttggtac acttttcggt aaagttggct gaggccgttt
     6421 tattgacggt gtgtttgctg cgagttttgg cgtttttaga agcggtaatg gaagattttt
     6481 taacggaaga agcggttttg cttttggtcg tacttttact ggcggctttt ttcgctgttt
     6541 tctgggtctt tttggtggtt gccgtgcttt tctttttatc tgcttttttg gtgatatgag
     6601 tagtagcaga actctccctg gcttgctttg aagcatgggc cataggcgtt aaaggcaggg
     6661 tggtaaaaag taaagcacag agcgtgatcg aaatacggtt tatccgcgcc actgagcgtc
     6721 cctctgttaa ggcaggtcta tcatgcctgc gtctgattta caaaacttga tgattctaat
     6781 gcatagaaga agagaaagtt aatagctttt attgttaaaa aatacgtttc gtcggcgagc
     6841 gcaaaaaaat gcagcagtga aagctaaaaa tggttataaa tacgccagtt tgccgtgcat
     6901 cagggaagaa acgaatattt gaaatgcaac aaatagagca aagggataaa atagcaaaag
     6961 cgtgatgaaa atgttatttt ccgatgccgc actgacccgc tacaatgtca gattactgcc
     7021 gtaaagaagt ttgaggaagc aagacaatga gcaccactat cgaaaaaatc caacgccaga
     7081 ttgctgaaaa cccgatcctg ctgtacatga aaggttcacc gaaactgccg agctgcggtt
     7141 tctctgccca ggcagtccag gcgcttgccg catgtggcga acgttttgcc tatgttgata
     7201 ttctgcagaa tccggacatt cgtgcggaac tgccgaaata tgcgaactgg ccgaccttcc
     7261 cgcaactgtg ggttgacggc gagctggtgg gcggttgtga tatcgtgatc gaaatgtatc
     7321 agcgtggtga actgcagcag ctgatcaaag aaactgccgc taaatacaag tctgaagagc
     7381 cggacgcgga ataatccgtc ctgcaatact gacggatgcg aacgggcatc cgtcagtcct
     7441 ttctctatcc ccaatccagc ccttgtggcg aacttgaaaa tcccgcctca cgtaaccgcg
     7501 taaacatcgg cgtttgccgg accggtaggt cattcacttc tgttagcgta aagcgcagcc
     7561 gtggttcgcg actggacagc tgtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc
     7621 gccggtgaaa acacgtccgg caggggcgat gaagggtttg gattatttgc cattaacgct
     7681 attccccgtg aatatcatgc atgatgcgtg gaatagcctc actatagatg cctgaatcat
     7741 tgcatgtcat caggcatcga ctcgattaca cctctgcggc ggcaggtagt ggccagcctc
     7801 ccagacgttt ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt
     7861 acagcgcgga gtgcgcctgg gtgctgtcga agtccatgcc agctgtctgg caagcctttg
     7921 acaatacggt ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag
     7981 ggtggaacgg gttacgtttc agtgaggcgc gttctgcggc ggccatcata aagctgtgat
     8041 cgaaattggc gttgtgtgcc accataatgg cgcggttaca accgctcgct ttaatacctt
     8101 tacgtacaac tttaaaaatt tcgtgcagcg cctcgtactc gctgaccgcg ccgcgatcgg
     8161 gatcgtttgg gtcaatgccg ttgaaggcaa gggcctctgg ttgcaaattt gcgccgacaa
     8221 atggttcgac gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt
     8281 tcagggtgat ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt
     8341 caacatcgat cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag
     8401 cgttatcgga catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata
     8461 ttgcttgtcg atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt
     8521 tgcccagacc gcgaccggcg tctttctctt cgattaattc aattttgtaa ccgtccggat
     8581 cttccacaaa tgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt
     8641 tacccccgtt ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga
     8701 tgtgaccata agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa
     8761 tcactgcttc ttcggtttcc gggccgtaac caacaaacgc cagtgagtat ttgtattccg
     8821 ggttttcgct ggtacgcagc agtttcatgc ccagcacgtt ggtataaaaa tcgatggagc
     8881 gttgcagatc gccaacgcgc agcatggtat gaagaagacg catctttata tcctcaaaat
     8941 ggctaacaga ttcaaaaagt tgttttagta tagcggcggc tttgcgccgc tcgcaatgtt
     9001 ggattacagc gtcgggtaat cagtgtaacc ttcagcaccg ccaccataaa aactttcggc
     9061 acgctgcggg ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat
     9121 ccagtcacga ccaaatgcta ccgcatcaat taaccctttg ccgatcagcg tttcagcttt
     9181 ttctactgtg tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc
     9241 gcgaaacgca tcagtatatg gttcacctcc cgcccaatct ggttctgaca tatgaagata
     9301 agcaatgccg cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg
     9361 gccgttatcc gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc
     9421 accccattct tcaatcccgg catcgaccac ttccagcacc agacgcgcgc gattttccac
     9481 gctgccgccg tactgatcgg tacgatggtt tgaagaaggg gaaaggaact gatgcagcaa
     9541 ataaccgtga gcagagtgga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat
     9601 ggcctgacgg aaatcattga cgatacccgg aatctctccc aattcaagcg cacgcggcat
     9661 ggatgtttca acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc
     9721 gctaagtgct gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat
     9781 gcgtccggtg tgccacagct gtacggccat atgaccattt tcagcatgaa cgccagcggt
     9841 aatttttttc catgcggcaa tttgttccgg gctatggatg ccaggtgcac ctgcgtatcc
     9901 ttttgcctgg gcagaaattt gcgtggcttc gctaataatc aaaccggcgc tggcacgttg
     9961 gcgatagtat tccgccatca acggggtagg gatgtcaccc ggttcgatac tgcgcagacg
    10021 cgtcagcggt gccataaaaa tacgatttgc cgccgtgatc gcgcccactt tcagtgggga
    10081 atacagtttt tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta
    10141 aacggcaggc gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa
    10201 actgcgcgaa attttggcct gcagattcgc gccaagccac agtgcgtaaa gcacttgtgc
    10261 ctgttgcagc ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc
    10321 ctgagagagc agggcgatca cgccgcgagc ccctttatcc attgcgctgc gcatatcttc
    10381 tgacagatcg cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg
    10441 ttggcaaaac tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc
    10501 ttcgccggat tgcagcaact cagtcagtcg ctggtgatat gcggcgtaat ggcgctcaag
    10561 catagcaacg ccaaacgctt ctttagaacg aaagtagtga tagaaggacc ctttcggcac
    10621 ttcagcggtt tttaggagtt cgcttagccc catcccggtg aatccacgtt gcaggcaaag
    10681 ttgctcgccc gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg
    10741 ttggagtgta gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca
    10801 gcttcaataa tggtaataat cttatctgag gagggcgagg tgacggagca attagagttc
    10861 tttcccgtcc agagcccgtg ccggggaatt tgccagtctg acgaacgggg attttgtcgc
    10921 ggctgttttc gtagccggga tgagcgtttt aactggaata agatgagcga tggcgaaaaa
    10981 caagaagtgt tgcgcctttg tcggcagcga ttaatgcgca aattacgtgt aaataagtca
    11041 gcgccatcag acgaaccgga gcaaccatca ctcttttgag cctaatcttg cgtatactcc
    11101 tctcatatct gataagagga agcaattatg gttcagcgta ttactattgc gccgcaaggc
    11161 ccggagtttt cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc
    11221 cgccagttgg tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat
    11281 gctgatattt atggtggcta tcagtgcgaa gcggcgtttg gcgaggcgct gaaactggca
    11341 cctcacctgc gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgtgt
    11401 gaagaaaacg tcattggcca ttacatcact gaccgcgatc acatcattaa gagcgccgaa
    11461 cagtcgctaa ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac
    11521 ccgttaatgg atgccgatga agtggcggac gcctttaaac atctgcatca gagcggcaaa
    11581 gtgcgtcatt ttggtgtatc aaactttaca cctgcgcaat ttgcgctgtt gcaatcacgc
    11641 ctgccattta ctcttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg
    11701 ctggatggca cgctcgacca actgcaacaa ctgcgtattc gtccgatggc gtggtcctgc
    11761 cttggtggtg gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct
    11821 gtggtggcag aggagttaaa cgcgggctcg attgaacagg tagtttacgc ctgggtacta
    11881 cgtttaccgt cgcagccgct gccaattatc ggctcaggta aaattgagcg cgtacgggca
    11941 gcagtcgaag cagaaacact gaaaatgacc cgtcaacaat ggttccgtat ccgtaaagcg
    12001 gcactaggtt acgacgtacc gtaagcactt ttaggaatag ctgccgttca aaaatgtgtc
    12061 actggtttac acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc
    12121 tggccattct ggcgctggtt gttgcgaccg gcgcacaagc tgccagtgaa aaagtcgaga
    12181 tgaacctcgt cacgtcgcaa ggggttgggc agtcaattgg tagcgtcacc attactgaaa
    12241 ccgataaagg tctggagttt tcgcccgatc tgaaagcatt acctcccggt gaacatggct
    12301 tccatattca tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg
    12361 cagaatccgc aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag
    12421 gtgccgggca tttaggcgat ctgcctgcac tggtagtcaa taatgacggc aaggcgaccg
    12481 atgccgtcat cgcgcctcgt ctgaaatcac tggatgaagt caaagacaaa gcgctgatgg
    12541 tccacgttgg cggcgataat atgtccgatc aacccaaacc gctgggcggt ggcggtgaac
    12601 gctatgcctg tggtgtaatt aagtaattaa gtaattaaga agccagcgta ccttgcggcg
    12661 gtgcttgctc aagttgcgaa agcgagcagt acagccgcca gacaattgcc gccagctctc
    12721 gggcggcagg ttgatgatga cgggcaaggc tgtcgcaaat ccgctgtaat tcttcaagtg
    12781 tggccgccag tgatttttgc tgaacgccac gctcactcat cactccccgc aacagtgaaa
    12841 tacagttatc ccgtactcgc gataacggat cggaacgcga ttcccagtcg cgcaattgcc
    12901 agacaacatg agaacagttc agcaacacta caccccagcg taataaccaa cggcgagcca
    12961 gcgcatcctg gctgttactc aactgactga cgtgatgata agtgagcgat tcaaattcgc
    13021 tttcactcag tgttggatgg cggcttagtt gatcgacaaa atcccggcgc agcgcgcgaa
    13081 tatggcggcg gcttttacga gcatccgatc ctggacgcag aatggcgaac gctaaccacg
    13141 ccaacgcgac gccaacaatt tttgccagat tatcgttaag aaaatcagca aaatcataca
    13201 ccgggggatt agtgacagcg ataaacgaac ccataaaaac aatcagttgc ccccacagtg
    13261 cggcaaattt ggacatctgc aatttaagaa gctgcatcgt cgccagcagt ggaaagagaa
    13321 acagtaaaaa ttgccacaga tcgctaatct gcaccatcag gccaaatttg accacaaagc
    13381 tgaatagcga aagtaacacc agcgtgcgca tcagcagtga caacgactta aacggtgccg
    13441 cgacggcgga gtagagtacg cagctaattg ctgccagcgt taaagcattg gcaccggcat
    13501 cccattgcga agcaatactc catgcgccaa tcatcattaa cgtacaaaat gtacgcagcc
    13561 cgctccacat agcttcggcg ttatcggtat gacgagccag gccggatgtc cgtgggagtc
    13621 tggcatgatc atctacaccg ctttgcagac gatgtaattc ctgactactt tgcagataaa
    13681 ggcggcaaaa ataacgtagt cgctgccaga aggcgacgtg ccggtagtcg gcgacgttgg
    13741 tcgggcgtag cggggagata atacgtgcga cggtgtaaac atctgtttgc gaactggcga
    13801 gcgccgtcag cagctgttcg agaatttctc gcgtggcatc tggcggtgag ggccagttga
    13861 gcaacatacg tcgcaggctg gagatgacac tggtcatacg taattgctgg tggagtagcg
    13921 cattaaggcg cgcgttttgc tggcgaaaac gatagtggct ccagaaagcc tggatacgca
    13981 gcaaattcat ggtcagtatc tgcccaatca ccccttcatg tgctgcacga atggcatcgg
    14041 ttgtttcagg ccgccagagt aaactggcat gttccagtaa tcgggcgtgc atgtttttca
    14101 atgcggttaa aagggctgta gcatcggaac tgctcggcag gatcatcatc atcatgccgc
    14161 cgcacaaaat accgactatc acctcgcaaa cacgcgcctg agcgatatcc cacagctggc
    14221 tggcctcagt aatattaacc atcggaaagg cgatgatggc agccgtgtag cccgccagtt
    14281 gaaatgcata cgcgacgtta ttcgtgaagt gcgcacaggc ccaggtacaa aagccaagcc
    14341 acgccgacat gctcaataga aaaaaccacg gctcattgag cgtatgtccg gcaagaagca
    14401 gtgccgcaat ggctccgagc aaactgccag cgatgcgtcc gaggcttttg ctgatgacac
    14461 cgccaacggt gggaaagcta accaccgcag ccgaggtcat cgcccaatag ggctcatcaa
    14521 gattcaaata ataggcaacc gtcagcgcca gacacatggc aatggtattg cgtaacgcat
    14581 aacgccattg cgccagcgtg gccctgaacc agggcaaatt gcgcaaggac catgacgatg
    14641 cgttcattat tgacctacgg caatgctaca ggtggtgccc gacaccagcg tgacatcgcc
    14701 tgggacttta tccagcgtaa agcggacggg aacgcgttgg gcgaggcgaa cccaggggac
    14761 gttaggtttc acatccggaa taaggcttga gtcactttca acgctctgat cataaatcgc
    14821 ccgaccgata ctggacacgt gaccctgtaa cgttttgtta tcactataga gggtaatttg
    14881 tgctggtgca ccttcgcgga tatggcgcaa cttggtttct tcaaaataac caatgacgta
    14941 aaacgagtgg ctgtcaacaa gggcaaacag cggtttcccg gtgtcagcgt agtcgccgat
    15001 gcgggtagtg agattagtca cccatccact caccggggcg cgaatttccg tttgtgccag
    15061 ttgccattgt gcctgtttca gcgtcgcctg tgcggcatca acgctggcct gcatcgcttt
    15121 aacattgagg ttagcggtat cgagttcttc ggcagaaata aaattttgtg agagatgacg
    15181 acgacgattc gcttcgttat tggcttttgc gaggccagat tgcgctttcg ccagctgagc
    15241 ctgtgcgttc agctcggcga tctgaaaggg cgttttatcg atcgtgagca gaagatcccc
    15301 cgcgttaacc agctggttgt cttttatatt cagctcaaca atacgtcccg acacctgtgg
    15361 ggtgatagaa acctgttctg cgcgtatttt cccgtcgcgt gtccacggag attgcatgta
    15421 aaaattccac agccaccatc cggcaagaac cgcaactacc gctacaatga ttgttgagaa
    15481 atacttaatt gttttaattg acatagttac cacgcaatca gtattgccag agcaaggcaa
    15541 acgcaaatcg caaacagcga taaatccatt aacaagggat gccagatgtc accggcgtag
    15601 atccagcctc gaagcaggcg gtgtatgaca agccagatga cgaatccaaa tgcgaatgct
    15661 ttgaaaaacg gaggaaagta gacggacgca ccgaacacca gatcttgtaa gggcaatcct
    15721 gttgcattga gcataaactt cacaaagtag attcctttac gacagaatat attgcgttag
    15781 attaataaat attctttaag tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga
    15841 cggtaaccaa atgcagcaat acatttgttt tagcaataca attgctgcac actattctaa
    15901 aagccgcata atatcttagc aagctaatta taaggagatg aaattggaat cgccactagg
    15961 ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc
    16021 gctggagtta acacaaaccc attgggttac gttacacaat atccatcagt tacctccaga
    16081 ccagtcgcaa attcaactgg caaaagcgat tggcatcgag cagccatcac tggtccgtac
    16141 gctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg
    16201 ggctaaacgt attaaactga cggaaaaggc agagccactg atcagcgaaa tggaagctgt
    16261 tattaacaaa acccgcgcgg aaattttaca tggcatctcc gcagaggaac tggaacaact
    16321 gattaagctc atcgcaaaac ttgagcataa tatcattgag ttacaggcca aagggtgaaa
    16381 tgaagggggc gtgtggtcag taacctgacc acacgcaaac ttatttagcg cggggaaacg
    16441 gtcacctgac tgccattgct ggccagtacg acacgttggc ctggagagaa atgagtgttc
    16501 ccttgtttct gtaccaccat gatggtgttg ccatcatctt tacgaatttc cagctcgaca
    16561 ccctgcgttt tgttcattgc actctgtacg ccctgaccgg ctacaccacc tgcaacagcc
    16621 cctgctgcag tagccagaga acgcccggtt ccgccgccaa tagtatttcc caggaaacca
    16681 ccaagtacag caccaccaat tgcgccgata acgttggaat catcaccgcc ctgaatctgt
    16741 acctgacgta cattaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa
    16801 gcggtataaa catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct
    16861 accattgaaa caaccaatac gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg
    16921 ccacgcaagc atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt
    16981 ctacc
//
LOCUS       DAZFRG010000100        16905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFRG010000100 DAZFRG010000000
VERSION     DAZFRG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.100"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..1194
                     /gene="arnB"
                     /locus_tag="ACHKUC_002560"
     CDS             55..1194
                     /gene="arnB"
                     /locus_tag="ACHKUC_002560"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO6618803.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1198..2166
                     /gene="arnC"
                     /locus_tag="ACHKUC_002561"
     CDS             1198..2166
                     /gene="arnC"
                     /locus_tag="ACHKUC_002561"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO6618804.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2166..4148
                     /gene="arnA"
                     /locus_tag="ACHKUC_002562"
     CDS             2166..4148
                     /gene="arnA"
                     /locus_tag="ACHKUC_002562"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO6618805.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4145..5035
                     /gene="arnD"
                     /locus_tag="ACHKUC_002563"
     CDS             4145..5035
                     /gene="arnD"
                     /locus_tag="ACHKUC_002563"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO6618806.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTVQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSH"
     gene            5035..6687
                     /gene="arnT"
                     /locus_tag="ACHKUC_002564"
     CDS             5035..6687
                     /gene="arnT"
                     /locus_tag="ACHKUC_002564"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO6618807.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDEMRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRNKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6684..7019
                     /gene="arnE"
                     /locus_tag="ACHKUC_002565"
     CDS             6684..7019
                     /gene="arnE"
                     /locus_tag="ACHKUC_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO6618808.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7019..7405
                     /gene="arnF"
                     /locus_tag="ACHKUC_002566"
     CDS             7019..7405
                     /gene="arnF"
                     /locus_tag="ACHKUC_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO6618809.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7399..7665)
                     /gene="pmrD"
                     /locus_tag="ACHKUC_002567"
     CDS             complement(7399..7665)
                     /gene="pmrD"
                     /locus_tag="ACHKUC_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO6618810.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7775..9130)
                     /gene="menE"
                     /locus_tag="ACHKUC_002568"
     CDS             complement(7775..9130)
                     /gene="menE"
                     /locus_tag="ACHKUC_002568"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO6618811.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AVQGVVEGSSVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVKRQ
                     Q"
     gene            complement(9127..10089)
                     /gene="menC"
                     /locus_tag="ACHKUC_002569"
     CDS             complement(9127..10089)
                     /gene="menC"
                     /locus_tag="ACHKUC_002569"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO6618812.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCEIPQMPSVAFGVSCALAELAETLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10089..10946)
                     /gene="menB"
                     /locus_tag="ACHKUC_002570"
     CDS             complement(10089..10946)
                     /gene="menB"
                     /locus_tag="ACHKUC_002570"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO6618813.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10961..11719)
                     /gene="menH"
                     /locus_tag="ACHKUC_002571"
     CDS             complement(10961..11719)
                     /gene="menH"
                     /locus_tag="ACHKUC_002571"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO6618814.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQEPAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11716..13386)
                     /gene="menD"
                     /locus_tag="ACHKUC_002572"
     CDS             complement(11716..13386)
                     /gene="menD"
                     /locus_tag="ACHKUC_002572"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO6618815.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13475..14770)
                     /gene="menF"
                     /locus_tag="ACHKUC_002573"
     CDS             complement(13475..14770)
                     /gene="menF"
                     /locus_tag="ACHKUC_002573"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO6618816.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14849..15154)
                     /gene="elaB"
                     /locus_tag="ACHKUC_002574"
     CDS             complement(14849..15154)
                     /gene="elaB"
                     /locus_tag="ACHKUC_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO6618817.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15209..15670)
                     /gene="elaA"
                     /locus_tag="ACHKUC_002575"
     CDS             complement(15209..15670)
                     /gene="elaA"
                     /locus_tag="ACHKUC_002575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6618818.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15735..16652
                     /gene="rbn"
                     /locus_tag="ACHKUC_002576"
     CDS             15735..16652
                     /gene="rbn"
                     /locus_tag="ACHKUC_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO6618819.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCGA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFNV"
     gene            16840..>16905
                     /locus_tag="ACHKUC_002577"
     CDS             16840..>16905
                     /locus_tag="ACHKUC_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618820.1"
                     /translation="MMVTVISNYCQLSQTQLCQTFA"
BASE COUNT         3897 a         4582 c         4328 g         4098 t
ORIGIN      
        1 cactctttat agcaaatata agtggacagg tattcaatga cggatggaaa aacaatgtca
       61 ggatttttgc ctttttcgcg accagcaatg ggcgtagagg aactcgctgc agttaaagag
      121 gttctcgaat ccggttggat cacaaccggt ccgaagaatc aggcgcttga acaagctttt
      181 tgccagttga cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat
      241 atcacgctaa tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc
      301 tgggtttcaa ccctcaatat gatttccttg ctgggtgcaa cgccggtaat ggtggatgtc
      361 gaccgcgata cgctgatggt cacgcctgaa gcgatcgaag cagccattac gccacgcact
      421 aaagccatca ttccggtgca ttatgccggt gcgccagcag atattgacgc cattcgcgcc
      481 attggcgaac gttacggcat cgcagttatc gaagatgctg cccatgccgt cggtacgtat
      541 tacaaagggc gacatattgg cgcaaaaggt actgctattt tttcgtttca cgccatcaaa
      601 aatattacct gtgcggaagg tggcctgatt gttactgata atgaaaatct tgcccgccag
      661 ctgcggatgc tgaaatttca cggtctgggt gtcgatgcct atgacagaca tacctggggc
      721 cgtgcaccgc aggctgaagt cttaacgccg ggctataagt acaatctgac cgatattaac
      781 gccgcgattg ccctgacaca gttagtcaaa ttagagcacc tcaacacccg tcggcgcgaa
      841 attgcccagc aatatcagca agcactggca gctctcccct ttcagccatt aagccttccc
      901 gcctggccgc acgttcatgc ctggcatctg tttattattc gtgtcgatga acaacgttgt
      961 ggtatcagtc gcgatgcgtt gatggaagcg ttaaaagaaa gaggcattgg taccgggtta
     1021 catttccgcg ccgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta
     1081 ccgaataccg aatggaatag cgaacgcatc tgttcgttgc cgctgttccc ggatatgacc
     1141 accgccgatg ccgaccgcgt catcacagcc cttcagcaac tcgcaggaca atgagccatg
     1201 tttgaaatcc accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa
     1261 agcttaccgg aattaatcag gcgcaccacc gcagcctgtg aatcactggg gaaagagtat
     1321 gaaatcctgc tgattgatga cggcagtagc gataattccg cgcatatgct ggtcgaagcc
     1381 tcacaagcgg agggcagcca tattgtgtct atcttgctta accgcaatta cgggcaacat
     1441 tcagcgatta tggcgggatt cagtcacgtc accggcgact taattattac cctcgatgcc
     1501 gatctccaga atccgccaga ggaaatcccc cgtctggtgg caaaagccga tgaaggttac
     1561 gacgtggtag ggactgtacg ccagaaccgc caggacagct ggtttcgtaa aaccgcttcg
     1621 aagatgatta accggcttat tcagcgcacc actggcaaag cgatgggtga ctatggttgt
     1681 atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgcagc
     1741 acctttatcc cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtgcac
     1801 catgccgagc gtgagtttgg tgaatccaaa tacagcttta tgcgcctgat taatttgatg
     1861 tacgacctgg tgacctgcct taccacaacg ccgctacgta tgttgagttt gctcggcagc
     1921 attattgcga ttggcggttt tagcattgcg gtgctgctgg tgattttacg cctgaccttc
     1981 ggaccacaat gggcggcaga aggcgtcttt atgctatttg ccgtactgtt tacttttatt
     2041 ggcgctcagt ttatcggcat gggattgctc ggtgaatata tcggcaggat ctacaccgat
     2101 gtccgcgccc gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat
     2161 gaataatgaa aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagccc
     2221 tgctggctgc cggttacgaa attagcgcta tttttactca tactgataat cccggtgaaa
     2281 aagcctttta tggttcggta gctcgtctgg cggcggaaag aggcattccg gtttatgcgc
     2341 cggataacgt taatcatccg ctgtgggtgg aacgcattgc ccaactatca ccagaggtga
     2401 ttttttcttt ttattatcgc catcttattt gcgacgaaat tttgcagctc gctcccgcag
     2461 gtgcatttaa tctgcatggt tcgctgttac ccaaatatcg tggtcgcgcg ccgctgaact
     2521 gggtgctggt gaacggtgaa acggaaactg gcgttacatt gcaccgaatg gtgaaacgtg
     2581 ccgatgccgg ggccattgtg gcacaactgc gcgttgccat tgcgccagac gatatcgcca
     2641 ttacgctgca tcataaattg tgccatgccg cgcgccagct actggaacag acattacccg
     2701 ccattaaaca cggaaatatt ctggaaatcg cccagcgcga aaacgaagcc acctgttttg
     2761 gtcgcagaac gccggatgac agtttcctcg aatggcataa gccggcatcc gtactgcaca
     2821 acatggtgcg tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt ggcaatcaga
     2881 aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag ccggggagcg
     2941 tgatttctgt tgcaccactg ctgattgcct gtggcgatgg cgcgctggaa atcgtcactg
     3001 gacaggcggg cgacggcatt actatgcagg gctcgcaatt agcgcagacg ctgggcctgg
     3061 tgcaaggttc acgcttgaat agccagcctg cctgtgccgc ccgacgccgt acccgggtac
     3121 tcatcctcgg ggtgaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag
     3181 atcattatga agtttacggt ctggatattg gcagcgatgc gataagccgt tttctgaatc
     3241 atccgcattt tcactttgtc gaaggcgata tcagtattca ttccgaatgg attgagtatc
     3301 acgtcaaaaa atgtgatgtc gtgttgccgc tggtggcgat agccacgccg attgaatata
     3361 cccgcaaccc gctgcgcgta tttgaactcg atttcgaaga gaatctgcgc attatccgct
     3421 actgcgtgaa gtaccgtaag cgaatcatct tcccgtcgac ttcagaagtt tatgggatgt
     3481 gtagcgataa atacttcgat gaggatcatt ctaatttaat cgtcggcccg gtgaataaac
     3541 cacgctggat ttattcggtg tctaaacaat tacttgatcg ggtgatctgg gcctatggcg
     3601 aaaaagaagg tttacagttc accctcttcc gcccgtttaa ctggatgggg ccacgactgg
     3661 ataaccttaa tgcagcgcga attggcagct cccgcgctat tacgcaactc attctcaatc
     3721 tggtagaagg ttcaccgatt aagctgattg atggcggaaa acaaaaacgc tgctttactg
     3781 atattcggga tggtatcgag gcgttatacc gcattatcga aaatgcggga aatcgctgcg
     3841 atggcgaaat tatcaacatt ggcaatcctg agaacgaagc gagcattgaa gaactgggtg
     3901 agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca ttacttccca ccgtttgcgg
     3961 gctttcgtgt tgtcgaaagt agcagctact atggcaaagg atatcaggac gtagagcatc
     4021 gtaaaccgag catccgcaat gcccgccgct gcctggactg ggaaccgaaa attgatatgc
     4081 aggaaaccat cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac
     4141 catcatgacc aaagtcggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg
     4201 cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag
     4261 cgtcggcccg gacaatatgg gccgccatct ctggcgactg gtgaagccac agtttttgtg
     4321 gaagatgctg cgctcaaacg cggcatcgct ttatggctgg gatattttac tggcaggtac
     4381 ggcctggcca ggtaaagaga ttggtcatgc caatgctgat atcattcgtg aagcggctaa
     4441 acatcacgaa gtcggcctgc acgcctggga tcaccatgcc tggcaagccc atagcggtaa
     4501 ttgggatcgg caaacaatga tcgacgatat tgcacgcggc cttcgcaccc tggaagagat
     4561 tatcggtcaa ccggtaacct gttctgccgc tgctggctgg cgtgctgacc aacaggtgat
     4621 cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg cgatgccgtt
     4681 tcgtccattg ctcgaatcag gaacccccgg cactgtgcaa attccggtga ccttacccac
     4741 ctgggatgaa gtgattggtc gggatgtgaa agcagaagat tttaacggtt ggttactcaa
     4801 ccgcatccag cgagataaag gcacgccggt ttataccatt catgccgaag ttgaaggctg
     4861 cgcttatcag cataattttg tggatctcct gaaacgcgca gctcaggaag gcgtgacatt
     4921 ttgcccttta agcgaactat tatcagggac gttgccgctc ggacaagttg ttcgcggaaa
     4981 tattgccgga cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc actgatgaaa
     5041 tcggtacgtt accttatcgg cctcttcgca tttattgcct gctattacct gttaccgatc
     5101 agcacgcgtc tgctctggca accagatgaa atgcgttatg cggaaatcag tcgggaaatg
     5161 ctggcatccg gcgactggat tgttccccat ctgttagggc tacgttattt cgaaaaaccc
     5221 attgccggat actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt
     5281 gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg cgctggtgac ctggtttact
     5341 ctgcgcttat ggcgcaataa acgtctggct ttactcgcca cagtaattta tctctcattg
     5401 tttattgtct atgccatcgg cacttatgcc gtgctcgatc cgtttattgc cttctggctg
     5461 gtggcgggaa tgtgcagctt ctggctggca atgcaggcac agacgtggaa aggcaaaagc
     5521 gcaggatttt tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc
     5581 gcccttgccg tgccggtatt aagcgtgctg ccatgggtag caacgcaaaa acgctggaaa
     5641 gatctcttta tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg
     5701 ggactggcga tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat
     5761 attcaacgct ttgcactgga tgatgcccaa catagagcgc cgttctggta ctacttgcca
     5821 gtcatcattg ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacgctggc
     5881 tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg
     5941 ctgtttttct ccgtcgctaa aggcaaattg cccacctata ttctttcctg ctttgcacct
     6001 ctggcaatgc tgatggcgca ttacgcgttg ctggcagcaa aaaataatcc tctggcgttg
     6061 cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg
     6121 gtctccccct ggggaccaat gaacacaccg gtgtggcaaa ccttcgagag ctataaagtc
     6181 ttttgtgcct ggtctatttt ttcgctatgg gcttttttcg gctggtacac cttaacaaac
     6241 gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg ggctggcgtt gctggtagga
     6301 ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca
     6361 caagaatcac ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt
     6421 ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa
     6481 tatggcctta attatccgga tgcaaaaggg agatttgtca gcggtgatga gttcgcaaac
     6541 tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt tgaccgcgat
     6601 gaagatatca acagtctcgc cattccgccc gcagatgtca tcgatcgtca ggagcgtctg
     6661 gtgctaattc agtatcggcc caaatgatct ggttaacatt agtctttgcc agcttgctta
     6721 gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgcggcggta aacaaacggc
     6781 gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggcctt gccatggtgc
     6841 tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga
     6901 attttgtctg ggtaacgctg gctgcagtaa aactgtggca cgagccggta tcgctgcgtc
     6961 actggtgtgg ggtggcgttc attattggcg gcattgtgat cctcgggagt acggtgtaat
     7021 gggcctgatg tggggattat tcagcgtcat tatcgcctcc gctgcgcaat taagcctggg
     7081 ttttgcggcg agtcatctgc cgccaatgac gcacttatgg gattttatcg cggcgctgtt
     7141 agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt
     7201 tttttgttgg tataaaacac tacataaact tgcgctaagc aaagcctatg ccttgctcag
     7261 tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt
     7321 ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct
     7381 gcccacaaca aaacaacgtt actgagtttt ccctgccgct ttacactgcc gttcccactc
     7441 atcggggcta taacagcttg cagaaaggac tttcaccgtg tgcagctttt cacgattaat
     7501 acagtaaagc gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact
     7561 cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa ttacatgtct
     7621 gttatcttgt ttattgcagc acgatttttt gaccagccat tccattgcat tatcctgttt
     7681 gctaagagtt ttcacatcaa tttacagatt aacagataaa ctctttatca acggcaatgg
     7741 aatggcagaa atgagaagtg aaacggtggg ttaattattg ctggcgtttc acccactctt
     7801 ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag ctccggcggc agagtcagcc
     7861 agcgcaccgg ttgttgaaaa cgagccagct tatctttcac ccattcgtca agatcaacgc
     7921 tttgctggtc atactccacc accgctaccg gccgatgacc aaactccttg tcggcaacgg
     7981 gaacgataaa cacctgcaaa accgcaggat gtgcagcaat aacgcgctcg acttcttccg
     8041 gctgaatacc ctcaccgcca ctgaagaata gattgtctaa acgtccgaca atggtcaact
     8101 tgccattatg catctcaccg cgatcgcgcg tggcgtacca gccttcgtca ttaaccagtg
     8161 gaacccgttg cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt
     8221 catcattaac gattttcact tcccgacccg gcagcggcga accaacgtct gccagaccgt
     8281 cggcttcttt cgcacacact gtggaggcaa actcggtcag accatagccg caaaagcacc
     8341 gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta
     8401 aaaccgcttt cagggaaacg gaactacggt taaccagcaa acgccagagt tgtgttggca
     8461 ccagtgaagc gtgagtacag cctgccagca tctgctccaa tggctgttta tcacgcaccg
     8521 tcatccgcgc accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca
     8581 gcggtaaaga gagcaaccag tcatcgtgat caccaaaagg aatcagcgat aacacccctt
     8641 cggcgctggc aagatgagcc tgataagtat gaacagcggc tttcggcaag ccagtggaac
     8701 cagaggttaa cgtcattgag cacagacgcg tcggctgcca cgccgcggca tgtgcgcctt
     8761 caaccagctg aatgtgcagc gacgctaacg ccggaaacgt gttttcccct tccggcacca
     8821 gcgcaaattg cagcgtaaga ttgggcagca attcctcaag cagcggttgc ggcagctgag
     8881 ggttcacggg caacacccgc gccccgcatt gcagtaacgc cagccaggcc agcagcgttt
     8941 gcggcgtatt ccacgcccgc aacatcacgc tgctgccctc aaccacaccc tgcaccgcaa
     9001 agctggaggc taattcatcg acgcgagcac aaagttcgcg ccagttgagt tgctcgtcat
     9061 tgagacgtaa ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag
     9121 agaagatcat aacagccgct ccagtgcatc aacgtccact aacggcagtg ggctccccgg
     9181 ccagcgacga acctgctgcg cctgcatcag atccagcgtg tccagtcccg gaatggtgtc
     9241 tggcgttaac caggcagcaa tccgcgccag ttgcgttaga cctaagctcg actcaatgga
     9301 agagctgatc accgccgtca gccccagcgc atgcgccgcc tgcacttgct cgcgtacttt
     9361 atccaggctg cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagctac
     9421 aaaggcaaaa tccggctcgc gcaagctttc atcccaggca atggcaatgc cggtttcacg
     9481 ggcaaacgca cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtg
     9541 gcgataatcc gggttaacgt atttggcaaa ctgctggcct ttcagcggtg tccaggcgcg
     9601 gttcgcgtca agacgcaaat gcagatccgg aattgcctcc agcaacagat tcaccaccat
     9661 gccgtcgcgc acggcttcgt acaatcccac tttgaccttc gccactttct cgccaggcat
     9721 atcggcaagt ttgaggatca gatcgtcagg atcgccatta cacagcggcg ccgcacggta
     9781 attggccgcc tgcgggagcg tctctgccag ctccgccaat gcacaactta cgccaaaggc
     9841 aacggaaggc atctgcggta tctcgcaatc gcctgccagc cagttattta cccaggcaag
     9901 cagcacactt tgcgcatctt cccaggtttc ctgactgaag cccggcagtg gggagatctc
     9961 cccccaccct tcgcgctcgc cctcacgcag gcaaacatac agcccgtcgc gggtttttaa
    10021 ccgcctgtcg cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc
    10081 gctacgcatt acggattccg tttaaatttg ctgaaatcag gctgacgttt ctggttgaaa
    10141 gcgttgcgac cttcctgacc ttcttccgtc atatagaaca gcatggtggc gttacctgcc
    10201 agctcctgca gacctgcctg accgtcacag tcggcattca gcgcagcttt caggcagcgc
    10261 agtgccatcg ggctgttttg caacatttca cggcaccagc gcacggtctc tttttccaga
    10321 tccgccagcg gtacaacggt gttcaccagg cccatatcca gcgcctgttt tgcgtcgtac
    10381 tgacggcaca ggaaccagat ttcacgtgct tttttctgcc caacaatgcg cgccatatag
    10441 gaagcgcccc agccgccgtc gaaggaaccg actttcgggc cagtctggcc gaagatggca
    10501 ttatcagccg cgatagtcag gtcgcacatc atgtgcagga cgtgaccacc gccgatggag
    10561 tatccagcca ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc
    10621 agcacgttca ggtgatgtac gccggaatca tctttatagc cgccgtaatc accacgtact
    10681 ttctggtcac caccggagca gaacgcttta tcgcctgcac cagtcagaat gatcacgccg
    10741 atgttgtcgt cataacgcgc atccgccagc gcctggatca tctcttttac cgtcagagga
    10801 cggaaggcat tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat
    10861 ttttcataac gaatgtcctc aaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa
    10921 agcattgctt catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg
    10981 ccagacttgc gattacgcca gcgggatttt cccggtgcgc gttatgtccg gcgcgaggaa
    11041 tgacatggca gtcggcagcc agttccgccg ccagagcgcg gaatttgctg tcacgttcac
    11101 cacataaata ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcgggctgga
    11161 cggcgagaga agtcgcctcc agcatggcgg caagggttgc gccattattg ttgctgcgca
    11221 gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc
    11281 aatcggcaaa taccgccgtt aacggttctg tacgaaaacg ctgcgcccat tggcgatcgg
    11341 aacgctgacg ttccgcgcgt tgttcagcat tttgcaatcc aggatgcccg ccttcgacaa
    11401 caactccaca aagccccgcc ggctcctggc aagccgccat catcgccacc cgtccgccaa
    11461 gcgagtaccc caccagccag aagttaagta tgttgtaact aaccagcgtt ttacacaata
    11521 agccggtgac atcatcaaat ccatcgacgc tgatcgtcgc tgaaccaccg tgacctggga
    11581 gatcaacata caaccgcgag tagtcggcaa acgcctcgcc cacttcttgc cattcatggc
    11641 aatcgccaga aaaaccgtgc aaaaacacca accagggtaa acctggtttg ccgtgttttg
    11701 cctgcgcgtg caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc
    11761 accatcggtg tcgttaacca ccatttcaat caccgtggtg gtcggcgtac gccaggcatc
    11821 gactagtgtc gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat
    11881 cgcagcggcg tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cgcgctcgct
    11941 tttcggcgtt ggcaacagcg aaaaaatttg cccgccgttg ttgttcacca caattaatac
    12001 cagcggtgcg gaaacctggc gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag
    12061 atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc tgaacgccgg cggcagtcga
    12121 gagcagcccg tcgataccgc tggcaccacg gttgctgtac accgggtaac ctgccggaag
    12181 ttgcgaaagc gcatcaatca gacgcaccac caggctgtta ccgacaaaca attgcccctg
    12241 ttcaggcaag tagtcgctga tgcgatgcgc cagttgcgct tcgccaaacg catcgcgacg
    12301 ggcaataacc gcctgcattg cctgttccgc caggcgcggg atttcaacgc accagggctg
    12361 gcgtttttct gccggatgca gctccagcca gtcggcaata ttggcaatta agcgacgtcc
    12421 gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca acaatccagt actcttctgg
    12481 ttcacagctt gcctgccatt ggaggagtcg tttccccgtc aggctgcttc ccagttgcac
    12541 cactatttgt gcctgttgca gttcgctggt cgctttggca ttgcctaacc agagatcggc
    12601 acacggcagc ggctgtccgg tttgcgacag cacgtcgcca atcagcggcc agccaagagt
    12661 ttgcgcccac agtgcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac
    12721 gccgcgcttt tgtcgccaga agaaccagtc acgctgtttt tcactttccc gacgaggcgc
    12781 ttcacgcagc cacggtttgt cgtcctgcca ccagtcgccc agccgctgtt gccagctaat
    12841 cccggtgtcg tccatttcgc catacagcgg ttcagcaaac gggcagttga tatggactcc
    12901 accagcatga agcgtaccga gagcgtggtc gatggtagaa accagccaac gtgcggggat
    12961 atcctgggtc gggcgcggca gtgaaatact gtgcgtgggg tgagaggcga acattcccgg
    13021 ctgacgaatc gcctgattcg cgccgcagtc aatcagctcc ggcgggcgat cggcggttaa
    13081 caggatcagt ttttctccgg ttaacccagc ttcaatcagt gccggataga gatttgctac
    13141 cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag
    13201 tgccagatgc cccagtccac gctcatcgaa atgggtatga tgaataaatg cggaattctc
    13261 cgccgccgct aaagttaacg gtgtagaacg cgagccaggg gcgatacaga tgtgtctgac
    13321 gccgtgacgc gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac
    13381 tgacatgagt ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat
    13441 tattgatatg aatcggtaat gatgcgactc attactattc catttgtaat aaagtacgca
    13501 gccctgccgc tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa
    13561 tgcctgcgcc agcatatagc cgcacgatat tgccgctgat ttttgctgag cgcagggaaa
    13621 cgcagaattc actttgctgt aatgagagat agcccgcaga accggcgtac cattctcgag
    13681 tgaacggttc gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg
    13741 ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc
    13801 agatacagcg gcgaagatgc tgtactttac gtagacgcag tacctgcggc ggtaaaacat
    13861 ccagcgtttg ggtatcggcc tgcaatcgtt ggcagatatc ttccaccacc aacatgttct
    13921 cgcgctggtt tttatcatcc gccatcagcc actcacctaa ctgctgcgcc tgcttatcat
    13981 caggatgatt tgctaccgtt cccgccagcg cttcggtacg cagcgctttg tcacgccgcc
    14041 gccataaccg ttccggtgaa gaaccaagaa aagcattttc gccatcaaag gccatgtaaa
    14101 aatggtagca attcagattg agccgacgac tggcagccat catcgctgcc gcattgaccg
    14161 gacttgcgaa atgcaggtcg gttgcccgag caagcacaac tttgtcgagc tcgccttcgg
    14221 cgatggtttt cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt
    14281 gttctcgcgt ggtggttaaa tgtaatccag gcaggggctt gatactcacc agtgtggcga
    14341 taaattcttt tgccttgatt gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc
    14401 gcagcgtggc tttaccgcca caacgtcgcc attccaggcg tggtaaaagt aaattgccct
    14461 gcgacgggtc aaacgcattc aacccccaga tgcgtaagtc ggcgtgttcc gggtgctggc
    14521 gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt aatcgcaccc aggacggcag
    14581 cttcttcatc accattacgt tgttgccagt agaattgcgg atacacctgc tgactggcca
    14641 gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc
    14701 cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa
    14761 gtgattgcac gcggacctct ccctgttaaa agcctcacat tatacggggt acgacaaaaa
    14821 aaatgcagta ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cccagaacca
    14881 gcccaacggc cgcacccaca ccaattcctt gccagggttt ttcgtggacg tagtcatcag
    14941 cacgataaac agcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt
    15001 ttttcacatc atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcgg
    15061 cgggatcgcc agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcat
    15121 catcaatacg tgtatcacca aactgattag acattctcgt tctccatttg tgtaaaacct
    15181 gtcactaact atagacaagg gttattgatt acgcctgaat tacctcgcgt gccatcccaa
    15241 tgtgtgggat accatcttct tcataaatat cggtcacagg gatgaaacca aattgcccat
    15301 aaaagttttg taaatgcgcc tgcgccccca gataaatcgg cttctccggc cagcggcgag
    15361 tacacgattc cagcgcctta ctcatcaatt gctgaccgat tttttcaccg cgtaaggctt
    15421 cgctgacaat cacccgacca atcacaacgg gttgcagttc gtcgtcactt ttcagaatcc
    15481 tcgcatacgc caccagtgtc ccgttatgcc aaccgagaat gtggcgattt tctccctcca
    15541 gatcgtcacc atcgatatcc tggtaaggac agttttgttc cactacaaat accgcacaac
    15601 gcagttgtaa taaggcataa agctgagaaa cggataaatc agaatgatgc agatcttgcc
    15661 agtcgatcat ggaaggctcc tttgtgctgt ttgctttata ctagccggaa tattttcagc
    15721 agcgcgatga actcatggaa ttaatttttt taggtacttc agccggtgtg ccaacccgta
    15781 cgcgtaatgt cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt
    15841 tatttgactg cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggaa
    15901 aactggacaa gatttttatc agtcaccttc atggcgatca tctctttggt ttacccggct
    15961 tactgtgcag tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcacg
    16021 gtatccgtga atttgtggag accgcgctgc ggattagcgg ctcatggacc gattatccgc
    16081 tggaaattgt cgaaattggc gctggcgaaa ttttcgatga tggcctgcgc aaagtgaccg
    16141 cttatccgat ggaacaccca ctggaatgtt atggttatcg tattgaagaa catgataaac
    16201 caggtgcatt aaatgcccag gcattaaaag ctgctggcgt gccgccaggc ccgctgtttc
    16261 aggaattaaa agcgggcaaa acaatcatgc tggatgatgg aaggcagatt aatggcgcag
    16321 attacttagc cgctccagtg ccgggtaaag cgctcgctat tttcggcgat acgggcccct
    16381 gcggtgccgc gctggagctg gctaaaggcg tcgacgtaat ggttcacgaa gcgactctgg
    16441 atatggcgat ggaggccaaa gccaacagtc gcggccatag ttctacacgc caggcggcgg
    16501 cactagcccg tgaggctgga gtcggtaagc taatcattac ccacgtcagc tcgcgctatg
    16561 atgacaaagg ttgtcagcac ctgttacgtg aatgtcggtc aattttcccg gcgactgaac
    16621 tggcgaatga tttcgcggtg tttaacgttt aactctgctt attattaaaa acagggcgaa
    16681 acttgccctg ttatcgcaac ccgcgctaca tatatccacc cacacgggta tattgagcga
    16741 ttttttatat ttattcatct cctcatgcac tatattcaga gctataagtt gcctcttttt
    16801 atcttgattc agcaccacct ttgcaactaa aaggagccta tgatggttac agttatcagc
    16861 aattattgtc aattatctca aacccaactc tgtcagacat ttgca
//
LOCUS       DAZFRG010000101        16853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFRG010000101 DAZFRG010000000
VERSION     DAZFRG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.101"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1739..1957
                     /gene="ccdA"
                     /locus_tag="ACHKUC_002578"
     CDS             1739..1957
                     /gene="ccdA"
                     /locus_tag="ACHKUC_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO6618821.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            1959..2264
                     /gene="ccdB"
                     /locus_tag="ACHKUC_002579"
     CDS             1959..2264
                     /gene="ccdB"
                     /locus_tag="ACHKUC_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO6618822.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            2265..3074
                     /locus_tag="ACHKUC_002580"
     CDS             2265..3074
                     /locus_tag="ACHKUC_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6618823.1"
                     /translation="MSGSVIHSQSAARVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGALINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPLERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAATDGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
     gene            complement(3212..3487)
                     /locus_tag="ACHKUC_002581"
     CDS             complement(3212..3487)
                     /locus_tag="ACHKUC_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6618824.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(3481..4125)
                     /locus_tag="ACHKUC_002582"
     CDS             complement(3481..4125)
                     /locus_tag="ACHKUC_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6618825.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            4354..5325
                     /gene="parM"
                     /locus_tag="ACHKUC_002583"
     CDS             4354..5325
                     /gene="parM"
                     /locus_tag="ACHKUC_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO6618826.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVVD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            5330..5722
                     /locus_tag="ACHKUC_002584"
     CDS             5330..5722
                     /locus_tag="ACHKUC_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6618827.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRTVLTELGASQ
                     SVEKMPPSATDSVQEAMNDVRLKMKKLF"
     gene            complement(5727..6997)
                     /gene="umuC"
                     /locus_tag="ACHKUC_002585"
                     /pseudo
     CDS             complement(5727..6997)
                     /gene="umuC"
                     /locus_tag="ACHKUC_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(6997..7431)
                     /gene="umuD"
                     /locus_tag="ACHKUC_002586"
                     /pseudo
     CDS             complement(6997..7431)
                     /gene="umuD"
                     /locus_tag="ACHKUC_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
     gene            complement(7428..7676)
                     /locus_tag="ACHKUC_002587"
     CDS             complement(7428..7676)
                     /locus_tag="ACHKUC_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6618828.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKGKERIQSLLEEIWQDDSWLPAA"
     gene            8094..8996
                     /locus_tag="ACHKUC_002588"
     CDS             8094..8996
                     /locus_tag="ACHKUC_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6618829.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVLSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLLDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYRWYTEQYGVKWPVGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            8993..9304
                     /locus_tag="ACHKUC_002589"
     CDS             8993..9304
                     /locus_tag="ACHKUC_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618830.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVKVYASPSPFTDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            9380..10063
                     /locus_tag="ACHKUC_002590"
     CDS             9380..10063
                     /locus_tag="ACHKUC_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6618831.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            10064..10285
                     /locus_tag="ACHKUC_002591"
     CDS             10064..10285
                     /locus_tag="ACHKUC_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618832.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            10215..10733
                     /locus_tag="ACHKUC_002592"
     CDS             10215..10733
                     /locus_tag="ACHKUC_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6618833.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RALLKNNTHENN"
     gene            10778..11548
                     /locus_tag="ACHKUC_002593"
     CDS             10778..11548
                     /locus_tag="ACHKUC_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618834.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVFF
                     PYAGEWLTEDEIRAVLDAVRDAVRSVSCRVAEDAQRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            11960..12385
                     /locus_tag="ACHKUC_002594"
     CDS             11960..12385
                     /locus_tag="ACHKUC_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6618835.1"
                     /translation="MQYAKPVTLIVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            12432..12854
                     /locus_tag="ACHKUC_002595"
     CDS             12432..12854
                     /locus_tag="ACHKUC_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6618836.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            12851..13042
                     /locus_tag="ACHKUC_002596"
     CDS             12851..13042
                     /locus_tag="ACHKUC_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618837.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            14005..14235
                     /locus_tag="ACHKUC_002597"
     CDS             14005..14235
                     /locus_tag="ACHKUC_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618838.1"
                     /translation="MTISIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            14287..15648
                     /locus_tag="ACHKUC_002598"
     CDS             14287..15648
                     /locus_tag="ACHKUC_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO6618839.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSEHRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            15695..16258
                     /locus_tag="ACHKUC_002599"
     CDS             15695..16258
                     /locus_tag="ACHKUC_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6618840.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            16258..16476
                     /locus_tag="ACHKUC_002600"
     CDS             16258..16476
                     /locus_tag="ACHKUC_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618841.1"
                     /translation="MTQGWREDKCPSVRAGKRPRAGGAERGCAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLFFFLPL"
     gene            16410..16610
                     /locus_tag="ACHKUC_002601"
     CDS             16410..16610
                     /locus_tag="ACHKUC_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618842.1"
                     /translation="MSVTDDVVVPATATALLFPAALILTTAVLSCLPGAPSAPGCLAA
                     SGRQARLRQAAAGAPASLRRTR"
BASE COUNT         3834 a         4245 c         4634 g         4140 t
ORIGIN      
        1 cactttaaat gaagttaatc tactggagat gaagtaactt cattaatgcg tcataaaaat
       61 aaaaattcct gttttgggaa atatgaatgt gtctgtcccc tccttcggga gggtgttttt
      121 tatctggaaa ctggtttctg gttctgattt ttctttttgt attatagcga gttacgcata
      181 tttaccaatc gatgtctggc aaacagagta tggcgatggt gtgatttgtg tttattggta
      241 aaaaaaacac ccccgtagtg ggggtgttga gacttcaacc tatgaactca ttatcctggg
      301 atggatgagt agcgtgcgat attgaatcat attcagcagg aataatattc gcaaaaaaat
      361 ggatgtgcaa ggaaattttt tatatgcttt tgtgtgtggt ggttaggtaa tcaagaagat
      421 aaatgatgaa gtgtgccagt tcaggatcaa tgtttccatg ggaaagccat ttattaataa
      481 tatcaattaa gttcgaaagc tcagggaaga tttgttcaat gttttcctct gtctcccgca
      541 cagtcatata gcgacgtctc cttggggatt gaaaacaaaa acaataatac aacaagtatg
      601 taacagaagg taggtggcag gggcggaaca ggaaaacgca tgagggcgct tcctggggga
      661 gatgcctggt atctttaggg ttcagttttg agatcgggaa tttttgtaca ttaagtgcat
      721 tatttaatca attgaaacgc gtaatggatt gattttatca gcaactatcg cgtttaagtt
      781 gtcgatcata tttgagttgt ctcattaagg tcgaatcccc tgaaagcggc gtataacgta
      841 ggagttcccc tctatagcaa taggtaaggc tgttcaaaaa aggcttaccg tacaataatt
      901 cgctaggccc cccttgtcat acaagctcgt aagcggccct gacggcctgt caatgcggta
      961 ctgccctgtc gggactgctc tacccacata agtttctaca tagcttgaag ctggatgggg
     1021 aggtattacg gctttagccg taatactttc ttcgtcctcg cagcgctgac agttttcagg
     1081 gatcaaattt aggtccgcca tacactccgt aaagactctg tcagttcaca ggtattacct
     1141 agtgaagacg tatcataaaa tgaaaactgt agttgggtag ttgctccccc tcacggcatg
     1201 caggggctga gggggaaaat ttccttatct tgttgttgct atttctggct tagtcagttt
     1261 tgaattttgc ttcctaagca ataatcccct caccgcaaca atcgttcttc tacactaata
     1321 agatccgaaa aaaggtagtc acggatcttc ctctcccgcc gcacagcgcc atctcccgtc
     1381 agcacaacat gtagtgctga atttggctgt taatgacact atatgttgtg tcatctccct
     1441 gacctgtgat gcgccgcgca ggggcggaaa acagtgatat gatgattttc tcagcgtggt
     1501 acacttccgg aaagttgtga tattaatgaa agtcggatct gacggaaacg ggaatgcgat
     1561 aattacggtt tgtttttata agtcagattt tgatttttat tggttaacat aacgcaaggt
     1621 aaaataaata aggcttacta aaagtaagat aacagtatgt gtatttacgc gctgattttt
     1681 gcatcataat aatatatact tatatgcata cccgaagtat gtcaaaaaga ggtgtactat
     1741 gaagcagcgt attacagtga cagttgacag cgacagctat caattgctca aggcatatga
     1801 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     1861 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     1921 gaacggctct tttgccgatg agaacaggga ctggtgaaat gcagtttaag gtttacacct
     1981 ataaaagaga gagccgttat cgtctgtttg tggatgttca gagtgacatt attgacacgc
     2041 ccgggcgacg gatggtgatc cccctggcca gtgcccgcct gctgtcagac aaagtctccc
     2101 gtgagcttta cccggtggtg catgtcgggg atgaaagctg gcgcatgatg accaccgata
     2161 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagt caccgcgaaa
     2221 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccgtta
     2281 tacacagcca gtctgcagcc agggtaccgg cagtttattc tgccggacag tctccacaac
     2341 ttcctgttgt cattgattat ccggcagctc tggcacttcg ccagatgtcg atggttcatg
     2401 atgaactgcc gaaatatctg ctggctccgg aagtgagtgc cctgctccat tacgtcccgg
     2461 atctgcgccg caagatgctg ctggccacac tgtggaacac cggtgcgctc attaatgaag
     2521 cactggcgct gacgcggggg gatttttcgc tcgcgcctcc gtatccgttt gtgcagctgg
     2581 ccactctgaa gcagcggacc gaaaaagccg ccaggacggc aggaagaacg cctgccggac
     2641 agcagactca ccggctggtt ccgctctccg actcctggta cgtcagtcag ctgcagacga
     2701 tggtggcaac actgaaaatt cctctggaac ggcgtaataa acgaacaggc aggacagaga
     2761 aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg
     2821 ccgccgctgc tactgacggt gtgacgttct ctgtcccggt cacgccacat acgttccgcc
     2881 attcctatgc gatgcacatg ctgtatgccg gtataccact gaaggtcctg cagagcctga
     2941 tggggcataa gtccatcagc tcaacggagg tctacacgaa ggtgtttgcg ctggatgtgg
     3001 ctgcacggca ccgggtgcag ttttcgatgc ctgagtccga tgcggtcaca atgctgaaaa
     3061 acagacacgc ataattcata aatcataatt atgaattgtg atttattctg taaaaaaaga
     3121 gaccactgca atatgtgatc tcttgtatgc aagggtgctt aaacagtatg aattcacaag
     3181 tgtgatttca taagtaataa cttattgatt attacgttag tgtttttaag tattgctcaa
     3241 gggctactct gacgatggca cttacatttt ttggatcttt accattgcgt ttattcctca
     3301 atgccagatc ttctattgtg acaagcatgc tttcaccaag agagattgtc gtccgacatt
     3361 gtttttctgg ttcgggtttt tccggcgcgc cgtatggttt atccgcaagg cgctgagcca
     3421 gagcctctgc ttgttctacc gtaactgcag ctctgtttaa tgcttgctgg ctgggtttct
     3481 tcaccatggg ccaaatacct cattcattaa atgttcaatt tctgctttgg ctgctgtatt
     3541 gttcgtttct actacaccgg tgccgttgct catgcaatcg cgatagactt ttctgaaaca
     3601 aataactgaa tcaaggactt gaattgttgg gaattcttca agatattcaa ggaattcttt
     3661 tctctcattt cctctcaaaa ccgggttggt tgttgccata ctttgtaaac aataaacctt
     3721 aagttccggg ttaaggttac gcattgcgtc tatctgttgt tctagttctg taagagtatc
     3781 cagatctggt tgagaacact gtagtggcgc aattatttga tgtgcgacaa ctccactcgt
     3841 tatgaattcc tttgagtttc ttcctgcgac atccactatt acatcatcga acttttcatt
     3901 cagagcctgt agtgtttgtg tcaggttatc aaatttctca acaagggtta ttgctggtaa
     3961 cagtcctgca gcttctcgtt ccgcatgatg ttttgcggct gttcgttgta gatctccgtt
     4021 taacaggcag acttcccttc ctttatttgc gagagcaata gccaggtttg atgcaatggt
     4081 agttttccct gaaccacctt tgttcccacc aactacagta atcattgcaa cctcataaat
     4141 gtgatgtgtg aagtatgatg atattttgac acggtaacct gaggtgtcaa tatgtgttca
     4201 tatttgtgaa gtatgaaatg tgaagtgtgc tatgtgatgt aataaataat acttgtgttt
     4261 tgtgatttca taactatgat ggtgctcaaa ttgagtatta ccttgcaacc agaaacaata
     4321 atttactcag attgagtatt taaggggcta tagatgaacg tatactgcga tgatggttca
     4381 acaacaatca agttggcatg gaatgataat gggaaaatct gtaagtcgtt gtcgcaaaac
     4441 tcatttcgtc atggatggaa ggttgacggg ttaggaattc gccagacatt taactatgaa
     4501 ctggatggta aaaaatacac gtatgatgaa gttagcaacc aatctattct tacgactcat
     4561 attgagtatc aatatacgga tgttaacctg ttagctgtgc atcacgcttt actgaatagt
     4621 ggattagcgc ctcagcctgt atcattgact gtgactttgc ctatcagtga gttctatacg
     4681 aaagagtgtc aaaaaaatga actcaacatt cagcgtaaaa ttgagaattt aatgaggccg
     4741 attcgtctta ataaaggcga tgttttcacc attgaacacg ttgatgtgat gcctgaatca
     4801 ttgccagctg tgttttctcg tctggtcgtg gataaagttg gtcagttcga aaaatcgctg
     4861 gtagttgata ttggcggcac tacgcttgat gttggtgtta tcgttggtca atttgactct
     4921 gtaagtgcca ttcatggtaa ttctggcatt ggtgtgtctt cagttacgaa agctgcaatg
     4981 agtgcattgc gcatggcatc cagtgatacc agtttcctcg ttgcagatga attgattaaa
     5041 cgtcgtaatg acccggactt tgttcggcag gttataaatg acgaaacaaa aactgatctg
     5101 gtccttaata ccattgaggg ggctattgcc agtttggggg agcaagtagt caatgagctc
     5161 ggtgatttcc atcatgtgaa tcgtgtttat gttgtcggtg gtggggcccc gttgatctat
     5221 gattcgataa aaacggcatg gcaccatctt gggcaaaaag tagtgatgat ggagtcgcca
     5281 cagacagcac tggttgaagc tatcgcggct ttcaaagagg agtaatattg tggacgatga
     5341 acggaaaagg aaaaaataca cactttatct gcatccggaa aaagctgcag actttcaaac
     5401 tttggaagcg atagagtccg ttccgcgttc tgagcgtggt gagctgttcc ggaatgcatt
     5461 tatttctgga atggcgttac accaacttga tcctcgcctg cctgtattgc tgacagccat
     5521 tttgagcgag gagttttcag cagatcaggt tgtaacctta ctcagtcaga cgactggatg
     5581 gaagccttct caggccgata tcagaactgt gttgactgaa ctcggtgctt cgcaatctgt
     5641 tgaaaaaatg cctccttctg ctactgattc ggtgcaggag gcaatgaatg atgtgcgtct
     5701 taaaatgaaa aaattattct gatatatcag aatgaagcaa tggggatctc agtccattgt
     5761 gttgtatacg aaggcgatag catttcccgt ttcatttgcc attcaggggc ggttcctcgc
     5821 cccgcaaacc acactttccc ttttcccgac tggttcagtt catcgagtgt tttcattaac
     5881 ttttcactgt ttttacgggg ctggatttca tcaaataatc ccggctgcgc tatacccgat
     5941 ggtgtgaaat cagccagcat gactcctgcc ttcatatagc ggtacccttc acgccagata
     6001 tggtttaaga ctctgcatgc ggcggcaata atgtcccggc tgtcctgtgt ggcaatgaaa
     6061 gcttttccac agcggcattg ctgtaacagg gttcttttac tgcaaagggg gatgtccgta
     6121 caaatgtcgt cacctgccga caatactgac gctccccacg tagtttctct gcggcccgct
     6181 ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt tcaccaaaac
     6241 tgcgactaca gacaatctgc tgttttgccg gtggtacttc ttccagggat atgctggact
     6301 cgccgttgag ttcgcgtacc gtacgctcaa gaatgacgct gaagtttttc cggatgaatg
     6361 ccgtgttagc ctgcgccagt tgcagtgctg tgttaatacc cagcgcattc agcttttccg
     6421 tcagtctgcg tcctactccc cagacctcac caactggctg cagccccagt agcttcaaga
     6481 tccgattacg gttttctgcc gtcagcgcga ccacaccgga aaactgtggc cattgctttg
     6541 ttgcccactg tgcactttta gccagcgttt ttgtaggcgc aatgcccacc cccatggtga
     6601 gtcctgtcca gctctttacc tgttccctga gctgatgacc aaaaaactcc ggagagatgc
     6661 aatgatttat cccccgcaaa tcaatgaaca tttcatcaat tgagtagggc tcaactgcgg
     6721 gagaaagcga ctccagaaca gccataaccc gttggctcat gctgtggtac agcgcataat
     6781 tgctggaaaa tacatgtatt ttcttctcca ggcgcatttg tctcacctga aaccagggct
     6841 gccccattct gatgccaagg gcttttgcct ccgggctgcg cgcgatcaca cagccatcgt
     6901 tattgctgag tacgatgacc ggttcgttgc gaaggtccgg gcggaaaact ttttcacatg
     6961 aggcgtagaa actgttgata tcagccagtg caaacatcag cgtaactccc gcgtcctgtg
     7021 tatcacgtga gtgacaatac caaaaataca gatgttttcc ggatacagtg tgcggaactc
     7081 cgggctgtct gaaaccggct ccagtgccgg gcgtgggcgc aacagcagtc gtttgacggt
     7141 gaactcaccg tcgatctcag cgataacgat gtccccgtgt tgtggttttt cggccctgtc
     7201 cactaccagc agatcaccat tctgcacgcc agcctggttc atcgattcac cgctggcgcg
     7261 cagaaagaag gtggctgcag gtctgccgat gcaatagctg ttcagatcca gttcctgctc
     7321 agcataatca gtggcaggcg aaggaaaacc ggcctggcaa cgatcggcaa aactagttga
     7381 cgtctaataa tcgcctgaag ggtcagccgg acggtgataa acggtactca tgctgcgggc
     7441 agccagctgt cgtcctgcca gatttcttcc agcagagact ggatacgttc tttacccttg
     7501 tcgttgcggg ctccagtgac agatacagac ggggcgctgc cctggtctat acgcagccag
     7561 atttccgggt agtgtggttt cagacgttgt tcgatttcat tctgtagcgc ctgaagtgtg
     7621 cctgatttga ggttttttgt gctctggcgg tcgaaaagaa tttcaatgcg gatcatgttc
     7681 tcatctccct tttcttttgc tgtatgtata tacagtattc taataactga atccacacac
     7741 agtcaaatgt cattattgtg actaaaaaac gctcaattct ctgagggtgt ggctgtatgc
     7801 ctgctgtgga ctgctgtgtc cagcttatcc acagcatttt gtgcacggtt ctgtggacaa
     7861 aatacttggt tacccaggcc gtgccggcac gttaaccggg ctgcatccga tgcaagtgtg
     7921 tcgctgtcgc cggcctcctc acccggtcac gtttcgtcgt tcctcctcca cgcgctgcgg
     7981 cttcggggcc gcacctgcat tcgtatgcgg tcgcccggtt acaggtgcgg cacggcctga
     8041 tggaggccgt atgtgagagg agaattccca tgccaaactg gtgctcgaat cgtatgtatt
     8101 tttctggtga accggcacag atcgctgaga ttaaacgact ggccagcggt gcagtcacac
     8161 cgctttatcg ccgcgccaca aatgaaggta ttcagctgtt tctggccgga agtgccggac
     8221 ttctgcagac cactgaagat gtgcggtttg aaccatgccc cggactgacg gctgccggac
     8281 gtggtgtttt atcgccggag aatatcgcgt tcacccgctg gctgacacac ctgcaggacg
     8341 gtgtactgct ggatgagcaa aactgcctga tgctgcatga actctggctg cagagcggta
     8401 ctggacagcg tcgctgggaa ggattactgg acgatgtcag ggataccatc accgcacttt
     8461 tcaccgcaaa aagaggtgac tggtgtggct tctggagtaa cgaggatgta tcggtgtggt
     8521 ggaaccgtct gtgtgacaac gtactgccgg aaaaaaccat gccgtttgac ctgctgacgg
     8581 ttctgcccac ccgcctggat gttgaagtga atggctttaa cggtggtgtt ctgaacggtg
     8641 ttccttctgc atatcgctgg tatacggaac agtatggcgt gaagtggcct gtggggtatg
     8701 aggtgaatat cagtagtcag ggagacaact tcattaaggt ggatttcgac acgccgtggt
     8761 gtcagccgga aagcgacgtt attgcagaat taagccgccg tttcagctgc acgctggagc
     8821 actggtatgc cgaacagggc tgtgatttct gtggctggca gttgtatgag cgcggagagc
     8881 tcgttgatgt gctgtggggg gaactggaat ggtcttcccc gacagatgac gatgagctgc
     8941 cggaagtcac cggacctgcg tggatagtcg acaatgtggc gcattatggc ggatgaagta
     9001 tgacggagac gggcgggcaa ccgcccgttt cttttccggt aaaggatgtc gccgtgctcc
     9061 tctttttact gcgccggctg acgcggcgcg gcataaacgc tgcctgtggt cagtgtcccg
     9121 cgtccggcgg gcacgggacg ggcgctttta ccgctcccgg ctggcgtcgg tgaaagtgta
     9181 cgccagcccg tcgcccttta ctgatgaacg cccttcaagc cgctttcgcg gcatagcctt
     9241 gccgtcaaaa agacgacggc tgcggtattc cacggtcggc ctgacccgtt accggacgcg
     9301 gtgaacagcc cacaggcagc ggggaacggg caccacaggg gtgccctccc ggtgccctct
     9361 gtaagagaag gagttttgta tgtcccgttt tgtcctcggt aactgcatcg atgttatggc
     9421 ccgtattcct gataacgcca ttgatttcat actgactgac ccgccgtacc tcgtcggttt
     9481 ccgtgaccgt tccgggcgca ccattgccgg tgatgttaat gatgactggc tgcaacctgc
     9541 cagcaatgag atgtaccgcg tactcaaaaa agacgcgctg atggtcagct tctacggctg
     9601 gaaccgtgtc gatcgcttta tggccgcctg gaaaaatgcg ggattcagcg tggttgggca
     9661 cctcgtgttc accaaaaact acacatccaa atctgcgtat gtcgcgtacc gccacgaatg
     9721 cgcttacatc ctggcaaagg gcagtccggc ccggccccgt aaccctctgc cggatgtgct
     9781 gggctggaaa tattcaggca atcgtcatca cccgacggaa aagcccgtta ccagcctgca
     9841 accactgatt gagagcttca cacatccgaa cgcgattgtg ctggacccgt ttgcaggcag
     9901 cggctcaacc tgcgtcgctg ccctccagtc cggacgccgg tatatcggta tcgagctgct
     9961 tgaacagtat caccgtgccg gacagcaacg actggccgcc gtgcaacggg ccatgcagca
    10021 gggggccgcg aatgataact ggtttgaacc ggaggctgcg taaatgaact atgccggaca
    10081 cgaaaaactt cgcgccgacg tggcggaggt ggccaacacc atgtgtgacc ttcgggccag
    10141 gctgaacgat atggagcatc gctgtcgctt tgattccgat gtgcttgttg aacgcctgac
    10201 acgccagacg ctgtatcgcg ccaatcgcct gttcatggag gcgtataccg aaattctgga
    10261 actggattcc tgcttcaaag attgagaagg agacgagaat gtacggaaca tgcgaaacgc
    10321 tatgccggga gctggcagca aagtatccgg gagacacgcc actgatgctg gttatctggt
    10381 ccccggaaga aattcaggcc ctcgctgacg gaatggatat ttccctgtcc gatcatgaaa
    10441 tcaggactgt cctggcgcgc ctggaggaca tcccggaaga ccagcggact gaatccggta
    10501 tttcttccgg cgtggcgatg gagatcatca ataacgtgag agaaaaccgc caggtgaccg
    10561 tccctgctga actgctggcg tccctgattc agaccgccga acaggcactg tggaaacgtg
    10621 agtgggccgc ccgtgataac ggcctcgccg tcccggaatg cgtcacccgc cgacaggcgg
    10681 tggttaatca ggcccgcgcc ctgctgaaaa acaacacaca cgaaaacaac tgatgttatc
    10741 gccgcctgcg ggcggcgatt cagggagacc atgaatcatg aacgaaacgc tgaatgcact
    10801 gatttatcgc cacgccagca acctgttgct ggcgcagggc tggccggaag agacggatgt
    10861 tgaccagcgt aatccgaagt atccgggctg gatcagcatt tatgttctgc tggatgcgcc
    10921 ccggctggcg acgttactta tcaaccgtca cggcggcgta ctgccgccgc tcctggcctc
    10981 cgccattcag aaactgaccg gaaccggagc ggaacttgtt ctgtccggca gtcagtggca
    11041 gtcgctgccg gtacttccgg cagacggaac acaggtgttt ttcccgtatg ccggagaatg
    11101 gctgaccgaa gacgaaatca gggcagttct tgatgcagtg cgcgatgcgg tacgcagtgt
    11161 cagttgccgg gtggcagaag atgcgcagcg tatcagggcg gcgctgacca caaccggtca
    11221 gacgttgctg acccgccaga cgcgccgctt tcgcctggtt gtgaaggaaa gcgatcaccc
    11281 ctgctggctc gatgaagacg acgaaaacct gcccgtggtg ctcgatgcca tcctgaaccg
    11341 gggagcacgt ttttcggcgg tggaaatgta tctggtcagt gagtgtgtgg agcatatcct
    11401 gtccagtggg ctggcctgcg atgtgctgcg tataccggat gaaccgcccc gccggtggtt
    11461 tgaccgtggt gttctgcggg aggtggtccg ggaagcccgg gccgaaatcc gcagcatggc
    11521 ggatgccctg gcaaaaatca ggggctgatg atggcgggga aaatccccgc ctttttcctg
    11581 ccggtcccgg acagagcaca aaggaagtcg ccgccggctc cgctttaccc ggccatgcgg
    11641 gcatggcctt atgggttttc agttatgtgg cctcagcgtc gtgtgcgggc tgtgccgtgc
    11701 ctccatcgta gccgggcttc tgcgggcggg ttgcaaggta aggtgaaccg ctgcggtcac
    11761 ccggtccctc cttcccgtct gccgtgattt tccggtcgtt cacccgctca gatttcgcgg
    11821 tctcaccctc caactcccgt cagccgtgtg cgtcaggctg cggcttccct tgcgcccttg
    11881 caaccctgca ggcccggcaa agatggaggc acggcaccgc ccggtgtcgc ggaatatgtg
    11941 aacaacggag gaaaacatca tgcaatatgc gaaacctgtc actctgatcg ttgaagagtg
    12001 cgaccgtctg tcttttctgc cttacctgtt tggcctggat tttctgtatg ccgaggcatc
    12061 tgtgtacgcg ctggcgaaaa aaatgatgcc ggaatatcag ggaggcttct ggcacttcat
    12121 ccgcctgccg gacggtggcg gttacatgat gccggatggc gaccgttttc atctggttaa
    12181 cggtgaaaac tggtttgacc gtaccgtgag tgctgatgcc gcaggcatca tcctgacctc
    12241 ccttgtgatt aaccgccagt tgtggctgta ccacgacagc ggggatgcgg gactgacaca
    12301 cttgtaccgg atgcgcgatg cgcagttgtg gagccacatc gaatttcacc ccgaatgtaa
    12361 cgcgatttac gcggcgctgg actgattaac ggacggggcg gcaatgccgc ccctgtaaag
    12421 gagacgagaa catgtactgt actgttaaag aaattatccg cgaagtgctg gatacagacg
    12481 tgccggacag tgaatgcgtt tttgccgtgg tgctgactcg tggggatgtg cgtcacatag
    12541 cccaggactg gagtctgaca gacgatgagc tggaaaccgt catgcagcgg ctggacgatg
    12601 cctttgagca tggtgcggat gtcagcgttg ttcacgacgt tgttcgtgaa ctgatggagg
    12661 aaaagcacgc cagccgtcag gtgacagtcc cggcggtgat gctggaaaaa gtgatggcgc
    12721 tggcaggcag tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg
    12781 atgcgttcgt cagggaagaa cgcgaagcaa tggacgttgt gttacaggcg ctagacgggg
    12841 agcacatgtc atgaatatca gcacagaaac ccgcgaaatt ctgcgtaatt acagggccgt
    12901 gattaatgcg cggcgtcgtg aaatggggca gaaaccgctc accaccgcgc agattgttga
    12961 tgaaatctgc gattttgtgg cgaatcagca ggcggttttc ctgggcggtc actacatctt
    13021 acagggcagc agaaacaggt gatacagggg caggcggtgg aggccgcctg tcagcccgcg
    13081 cgaacccccc tggcaggctg gcgcctgccg gaagggaagc gggccacgct ggcgcgtcgc
    13141 ccgttaccct tcccgtttgt gatgtgtccg gaatgttttt tcctgatgtt atgtttctgt
    13201 ggttactgtt cttcttcttc ctcttctttc cgttcctctc ctgtttatgg ctttcccggg
    13261 cgtcccccag gcggggcggg ctgtcgtgaa cggagccagc ggagctgtct ccgccctggc
    13321 gggcttccat ccctgacgca aaacccccgg cttgcagcga ccgttccggt cgcgccgccc
    13381 ctgtcgcgcc gtggcctgcg ctcctggcat cccggcaaaa agacgccggg atgtaaggcg
    13441 caacgccgtt tcgcgcacgc gctctccgtc gttgcggcgg gtccctgctc attgcctgcc
    13501 gggtgccaca cacccgtatg agtccctgtc gccgggggct gatacagcca caacctgccg
    13561 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa
    13621 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg
    13681 aaggttctca aacgggtgaa gttcatacca cagggaatac agcagcaggt tcccgcgaaa
    13741 cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc tttaaaaatc aggatttatt
    13801 tcaggctccg gcaatacgca tttatgtgtt ctgtcagtca tccggctggc aggtaatgaa
    13861 aacgcaggaa cagaaggagg aaaacaccgg aaaacgtcag atgcagatat gaaaaaagca
    13921 ggtcagggac ctgcttaatc catgccgcag agactgcacg aacagttttt gcggctccag
    13981 tcaatcgtca taagaggcta aattatgaca ataagtattg tttcaccttc cgcagcggct
    14041 gtaaagcccc gccgtcatcc ccgtttcaga cgcgaagaca tccccgcgcc ggaaattgac
    14101 cccgtgctga aagccttcgg gcgacacata gcccggagtt ttcaccgggg gcgtggtgta
    14161 cacatccccg ccatgaaaaa cacggctttc gggcaggtgc tccgcacact cgaactgaaa
    14221 cgcgctttta actgatgagg tgagccgccc cgcgaggggc ggcaacagac ggaaggagaa
    14281 cattttatga cattagcagc aatcaccatg accgccccgg aagccgccag ccctgtgcag
    14341 atgtaccgcg cgacctactc accggatgac aacaaactgc gcctgtatgc cgcgtcacgt
    14401 cttgacccgg agacgtataa aaaagtgcat gatgccggtt tccgctgggc acccaaacag
    14461 gcgctgtttg tcgcgccagc ctggacaccg ggccgggaag acgtgctcct ctcacttgcc
    14521 ggagagattg aggatgaaga cagcacgctc gctgaacgtc aggaagcacg ggcggagcgg
    14581 tttaccggat acagcggaaa gcgggccagt gaatccgcac aggcacttga tgaagtggaa
    14641 agactggccg cgatgatccc gcccggtcag cccattcttg tggggcatca cagcgaacac
    14701 cgcgcccgtc gtgatgcgca gcgtattgaa aacggcatga aacgtgccgt gatgctcttt
    14761 gaacgtgcgg aatactggga agaacgggcg cggtcggcac tgcttcacgc gaagtataaa
    14821 gaacgcccgg acgttcgctg gcgtcgtatc aaaaaaatcg aagctgattt gcgcaaggct
    14881 gaaaagacca tcgcacagtc gcagaaatat ctgacgatgt ggcgggctga atcgctggat
    14941 ctgaatatgg caaaactcat cagcagtcat gaccatatca gcgcctgttt cccgctggat
    15001 acgtatccgc gcccggcaga aaaaagccag tatgaaggga gccggtcgtt atggtcggcc
    15061 ctggatgatg acatcatcac cacggagcag gcccgcgaaa ttgcgatccg ctgtcatgaa
    15121 cggcagattc agcatcagca acgctgggtt aaccactatc agaaccgcct gatctatgag
    15181 cgtgccatgc tggacgaaag cggcggcgtg gttacccgga cacaggattt tgagccgggc
    15241 ggacaggttt tcagccgggg cgagtggctg actatcatcc gcgtgaacaa aagcaacggg
    15301 gcggtgagtt cagtcacaac gccaaattac agttttctcg ggtacagcgg cacgatgaaa
    15361 gtgacgcccg atcgcatcac ggactacaaa gcaccatcgg cagaagaggc tgccgtcgcc
    15421 agtcaggccg cgaagcgtcc gccggtagtc aactatccgg gggaaggttt ccgggaaatg
    15481 acaaaggcgc agtgggccgc cctgccccgg gactgtaagg ccgtgcgcag tgtggaagaa
    15541 gcagaagacc acggggcata ccgctaccgc cgcacaatgg acaataattt ccgtctggtg
    15601 aatgtgtata tcaccgacat gaaaatcacg gaaatcccac agaaataagg tatatccccc
    15661 cgggaaatcc ccgggggatg acggagataa tgacatgcac agtcagttaa gagaacgtat
    15721 ccggctgatg cgcgcaaggc tggataacgc cgcgccggtt gctgaaatcc gggctgaatc
    15781 tcagcttttt gtgagtcctg ccccgatctg tgatcgcctg gtgacgctgg cggaaatcag
    15841 caaccgtgat cacattctgg aaccctctgc cggcaccggg gccatactgc gggcgattcg
    15901 ggataccgcg ccggaggcca tgtgtgatgc ggtggagatt aacagcgggc tggtccggta
    15961 tctgcgggaa aattttaacg gtgtcagggt acagtgtggc gacttcatgg aatggcagcc
    16021 ggtgcagtat tacagccgga tcattatgaa tccgccgttc agtcacgggc aggatatccg
    16081 gcatatcctg cgggcttttt ccctgttgcg tccggggggc gttctggttg ccgtctgtct
    16141 caacgggcca cgccagcagg agaagctgtt accgttttct gacgtccgcg aggagctgcc
    16201 gcgcggcacg tttgcttata ccgatgtccc gacgatgatt attcgtttgc gtgcctgatg
    16261 acacagggct ggcgggagga taaatgcccg tctgtccgcg ccgggaaaag accgcgcgcc
    16321 ggtggcgctg aaaggggctg cgctcacgcg ccgccccttt cattacgcgg acgttctgat
    16381 gggggcttct gtcttttcag acctgtcatt tgtctgttac tgatgatgtt gttgttcctg
    16441 ctactgctac tgctcttctt tttcctgccg ctctgattct gactactgca gtgttgtcct
    16501 gtctgccggg cgcaccttcg gctcccggct gcctggcggc atccggtcgt caggcccgac
    16561 tccgacaggc agctgctggc gcacccgcca gtctccgccg aacccgctga cgcggtttcg
    16621 ggctgtcgct acggtccgat gcgtgaaacc ccccggcttg cagcgaccgt tccggtcgcg
    16681 ccgcctctgt cgcgccgtgg cctgcgctcc ctgcatcccg gcaaaaggcc gccgggatgt
    16741 aaggcgcaac gccgtttcgc aaaccgcgct tcacgtcgtt gcggcgggtc cctgcccgtt
    16801 gttgtgtccg gtgccattca cctgtttaag cccctgtcgc cgggggctga tac
//
LOCUS       DAZFRG010000102        16729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFRG010000102 DAZFRG010000000
VERSION     DAZFRG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.102"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..205)
                     /locus_tag="ACHKUC_002602"
     CDS             complement(<1..205)
                     /locus_tag="ACHKUC_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6618843.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEM"
     gene            complement(205..546)
                     /locus_tag="ACHKUC_002603"
     CDS             complement(205..546)
                     /locus_tag="ACHKUC_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618844.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(539..3781)
                     /locus_tag="ACHKUC_002604"
     CDS             complement(539..3781)
                     /locus_tag="ACHKUC_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6618845.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGGVNEASG
                     STKTLSGVLNGVAGHIDAVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQTTLD
                     NKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGLVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3829..4038)
                     /locus_tag="ACHKUC_002605"
     CDS             complement(3829..4038)
                     /locus_tag="ACHKUC_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6618846.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALNFAAQS"
     gene            complement(4134..4508)
                     /locus_tag="ACHKUC_002606"
     CDS             complement(4134..4508)
                     /locus_tag="ACHKUC_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6618847.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4526..5239)
                     /locus_tag="ACHKUC_002607"
     CDS             complement(4526..5239)
                     /locus_tag="ACHKUC_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6618848.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSFECTVTVSAAA"
     gene            complement(5305..5649)
                     /locus_tag="ACHKUC_002608"
     CDS             complement(5305..5649)
                     /locus_tag="ACHKUC_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6618849.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5646..6092)
                     /locus_tag="ACHKUC_002609"
     CDS             complement(5646..6092)
                     /locus_tag="ACHKUC_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6618850.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(6089..6439)
                     /locus_tag="ACHKUC_002610"
     CDS             complement(6089..6439)
                     /locus_tag="ACHKUC_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6618851.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDTT
                     GGRLEILCRLGGEK"
     gene            complement(6452..6778)
                     /locus_tag="ACHKUC_002611"
     CDS             complement(6452..6778)
                     /locus_tag="ACHKUC_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6618852.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(6775..9197)
                     /locus_tag="ACHKUC_002612"
                     /pseudo
     CDS             complement(6775..9197)
                     /locus_tag="ACHKUC_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(9143..9364)
                     /locus_tag="ACHKUC_002613"
     CDS             complement(9143..9364)
                     /locus_tag="ACHKUC_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618853.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            complement(9409..11346)
                     /locus_tag="ACHKUC_002614"
     CDS             complement(9409..11346)
                     /locus_tag="ACHKUC_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6618854.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRIREGLEITATLVKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIQTIKSFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGHGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGQGKARPLTKFDFESITFSHAKVAAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDTDAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            complement(11411..13072)
                     /locus_tag="ACHKUC_002615"
     CDS             complement(11411..13072)
                     /locus_tag="ACHKUC_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6618855.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
     gene            complement(13069..13632)
                     /locus_tag="ACHKUC_002616"
     CDS             complement(13069..13632)
                     /locus_tag="ACHKUC_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6618856.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(13924..14289)
                     /locus_tag="ACHKUC_002617"
     CDS             complement(13924..14289)
                     /locus_tag="ACHKUC_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6618857.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSDRADDIIGL"
     gene            14331..14531
                     /locus_tag="ACHKUC_002618"
     CDS             14331..14531
                     /locus_tag="ACHKUC_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6618858.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPTSMTNNEK"
     gene            complement(14730..14954)
                     /locus_tag="ACHKUC_002619"
     CDS             complement(14730..14954)
                     /locus_tag="ACHKUC_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618859.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(14951..15445)
                     /locus_tag="ACHKUC_002620"
     CDS             complement(14951..15445)
                     /locus_tag="ACHKUC_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6618860.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKNVREL
                     KLANAAITDMQMRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCTKLAFYY
                     PEDT"
     gene            complement(15447..15584)
                     /locus_tag="ACHKUC_002621"
     CDS             complement(15447..15584)
                     /locus_tag="ACHKUC_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618861.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            complement(15744..16277)
                     /locus_tag="ACHKUC_002622"
     CDS             complement(15744..16277)
                     /locus_tag="ACHKUC_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6618862.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKAKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(16341..16691)
                     /locus_tag="ACHKUC_002623"
     CDS             complement(16341..16691)
                     /locus_tag="ACHKUC_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6618863.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEVGGYPSLLLPDVAISLDR
                     TEGATLELYEAEAKKQAKQFFMDVAAGLCEGNEPLPEKRPVILEAQNVWITYKGNLPG
                     RITGSLKTPPESQP"
BASE COUNT         3511 a         5380 c         4009 g         3829 t
ORIGIN      
        1 tcatctcaaa accgctgccg tcaatcgggt atgcctcata cttacgcccc tgccaggtga
       61 ccggctcccc tttttcattc agctcattac agaaaaaata ccgctcaccg ccctgtaccg
      121 tcaggtcgat ttcccagagt accacccgcg gtgactgctc tgatttaacc gactcgttca
      181 gactttcttc gtgaatatcc tgcatcagtt caccacctgc tcaatcgtac aactgaaatc
      241 actgtacctg gcgttatctg tgacgctcca ctcccggcat accaccctca ccgtccggtt
      301 atgtttcggc ggtcgccaca aaaaggcacg gtaaccacca tgccaggata aaaattcatc
      361 cagccagcgc cgggttggtt catccgtcac ccggaacacc gcctgaaacg tcttcagtct
      421 ggcattaagt cccgtcggtc ggcgctgttc ataaccgtca ccaaaccgaa ccctcaccac
      481 cgacggtttc tcactcacct gcatcccttc acgcgggacc agatgcagcg tttttatctc
      541 agccactcag cattcctccg tcacgtcgca tggacagcat caccgcctgc acccgctggt
      601 caatcagctg cacaagactg cctgccgcct ccggccctat ctgtccgtta gccccgtcat
      661 tctgaatggc gatgtggtag accggggaat acaccagacc ggcactgccg ttcatactgc
      721 ccaccgcgcg tacgcccagc gagccatccg ccgcccgggt caggggcata atggcttcag
      781 gtccggcttc ccccatcagc ccggcccctt ttgcaaacgc aaagtacgtg ggcgtgtcca
      841 caatgctgtt gctgtacgca ctcaggtttg ccgaggtata cacgccgcct tttgcattgg
      901 ccaccgcacc gcccagccag tcaccaatgc tgccaataaa tcctcccgca ccggacatac
      961 cgtttgccgc cgtcttaatt ccgttgacaa tcgcggcatt cataagaact tttgagattt
     1021 cctgcagtac ggatgaggcc cagttgcgcc attccacttt gtttccgttc agcatctccg
     1081 tgatgttatt caccagtcct gaaatcccct ccgttgccag ctgtgctgcc tgagaggcgt
     1141 aatcggatgc attgtccacc cagttactga gcccctcctg caagcctttc tgccagtccg
     1201 cacgctgcgc atccgattcg gcataaaagg ctgcctggtc cttaaggcgt tcgctcagat
     1261 actgcgcgtt ctgtgcccgt gcctgtctgt aaaaatcctc actgatatcc ccggtctgat
     1321 actgagactg aaggtccgca tccttctggc ggaagctgtc gcggatctgc tgcaactccc
     1381 gcatgcgttc cctggctcgt tctccctgcc cgtaccccag cagttcggct tcatttgatg
     1441 cacgcgcagc cacattatca ttcttcaggg tctcttcccg ggatcgcaac tgttcccgga
     1501 ttttttgctg gtcaatcagg gccgcgttac gcagcagttc ctgcttctgc atctccgtca
     1561 gggttttcag ttctccctgc gcagtctggt acttcagctt cgccagttct gtattctgac
     1621 ccgccagtgc cagttgctct ttctgctgtt tcagtagccg ggaaaaactg tcttccgctt
     1681 tttccgtctc tgattttcca ccccgggatt taggtttgtt cgcctcgtta ttacgccagg
     1741 cttccagagc attactgata taacgctgtc tcgcctcctg atacgaatcc cccacaaaac
     1801 cgaggtcatc ggccgcatac cccagccggg cacgctcttt ttcttcccct ttcagtctgg
     1861 acagggccag ctcacgctct gtttttgtca gggcgctctg ctgtttatta tccagagtgg
     1921 tctgtggcag ccgtaacggc acattcacca gtccctgccg ctgctgaagc agttcattcc
     1981 ccagccccag cagacggttg aattccgtat gctgaccgtt cataaccagc atggactggt
     2041 acaccttatt ctgctctgcc gcctgctgac gaattaacgc cacacgacgg tcttccagcc
     2101 cggcaagcac atcctgaatg gactgcgctt tttcctgcat ctgtgccaga cgggactgct
     2161 caacggcaag ctgctctgtt gcctgagaaa gcccttccgt tacggtcttc accgatgtca
     2221 gatggtttat catgaatccg tcaccggttg tccagccagg gttagccaga acatactgat
     2281 atccagcgat tttttcctgc agggatttca cccggctggc ctgttcatca atcagccggt
     2341 tctgctctgt cagcgccgcc cgtgttcgtc cttcattatc tgaggcttca ggcaaagaca
     2401 ttgacggcgt tttatgcgcg atttcatcta tcgtcagtgc atactggcgc gctgactccc
     2461 tggcctgctc ctgattctgg tacagcgtat accatgctgc tgcccccagc atcaccagtc
     2521 cgggtacgcc accaaccagt cccaacgcac cagtcatcag acgtgaaccc accgccgttg
     2581 tactgttcag cgcattctgg gctgcggttc tggcagcaat atttctgttc aggcgttcct
     2641 gtgtggccgc cagacgggct tctgcagcaa tctgcatctc cgtcccgcgg gctgccgcca
     2701 cagcctgctg tgcacggtac acggctgccc ttgcccgcgc cgtggcaatc tgcgtccccc
     2761 tgagctgtgc ttccgccagt gccacttcat tacgtgctgc cgtcacaagt cctgccgtgg
     2821 cagacaccgc tccggaggcc atattgccaa agtaccgggc aaccccgacg gcaaccagcg
     2881 cccccgctgc tgttgccact gcatcaatat gtccggccac accattcagc acgccggaga
     2941 gcgttttcgt cgagccgctg gcttcattca caccgcccac ccaggccata aaggcgtttt
     3001 ccaccttcgt gatactaccg gaaaccgttt ccggcatggc cgcatattca tcacgtaata
     3061 tccccagctg gctgattaac gcagggacca ctttatccgc tgtcagtttt ccgtcatccg
     3121 ccattgcctt cagatcttta cgggccacac ccatccccgc agcaagcgcg cggatcaccc
     3181 ggtcgccatt ttcgttaaca gcattaaatt cttcgccacg cagtacaccc tgtgccagtg
     3241 cctggctgaa ctgggtgatc accgaaccgg attccgcaac tccagcccct gacagtttca
     3301 gtcctgtcga aatggcctcc gtcaccttca gcacatcatc agcactgtaa ccatattcac
     3361 gcattgaggc tgccgagcgg gcaaacaggg ccgcattatc tgaaaatgcc gtgcccgtcc
     3421 gctggctgat atccatcagc actttctgtg atgacgaaaa ttcatcggat gactgcgacg
     3481 cctgtttcag tcgggcattc acggaactcc attcatcggc cagagaaatc aggtgcccgg
     3541 tggcaaaggc accggcaaat gcccccgccg ttccggcagc tgaagcgcgg atttccgtca
     3601 actggctgtt cagctcagcc agggcgcgtc gctgctcccg ggcgactgcg gcagcctgac
     3661 gcccgccatt ctgcagggtc cgataatatt cactgcccat gcgggaagcc cgctggatct
     3721 ccgactggaa tgactgcgaa tttgccgaaa ttttgataat cagttcacgt aacgtcgcca
     3781 ttcacctttc tccgggcgta aaaaaaccgc ctcagcggtt ctcatcattc atgactgtgc
     3841 tgcaaagttc agcgcgtctt ccagcgccgc aaacggatcc acctccggct tatcctcatc
     3901 ctcgccccag cagagcatgg cgtccttcag tgcaacattc atcccctgtg ccccgaaaac
     3961 cgctttcacg atctgtgcat tacggatatc cccgcgctca tcacccagcg gggataccct
     4021 gtcgaactcc atccacatca tcgcctcgct cgcactcagg ctgtgccgca gttcggataa
     4081 ggtgcgcccc agacggagcg caagtcgcat cagaaagcga atttccgggc gggctacttt
     4141 tttctggccg actctgcatc agcgatcagt tccagtgcct gacgcagcaa ccgggcatgt
     4201 accggaccat agacggccag cacctgctca cggtcgtccg gagtgaacac ccgctgcaga
     4261 tccgtatcac acaggacatc gcagaacagc gtcacatccg cttccaggtt acggcgggtt
     4321 ttcgccacca ccgacagggt atcgtcatcc tctccatcac cattgagcac ttcctgccac
     4381 agataccagg cctctgccga aggctcccgc agcaccacgc tgacattacc ccattccggc
     4441 accttcaccg ttttatgacg aaaccctgac agtctggcca gcgccagcgt tttcagatcc
     4501 tttttcatga tgacccatcc ccttatcagg cggctgcgct cactgtcacg gtgcattcaa
     4561 aagacgtcac actctgtgct ttctctgccg aatcggtcac cacgcaggta tatttccccg
     4621 catcagcgga ctgcgcacct ggcttactga aggtgtctgt cgtctgcccg tcaaccggct
     4681 gaccatcctt cttccaggcg tatttatacg gcggcgttcc cccgttggca ctgactgaca
     4741 ttgtcagcag cgcaccggta ttcacggtaa gtgttttatc cagatttttc acaaacgcca
     4801 gcggtaccac aaaggacacc ggtttgcctt tcagacgcag tgagaacgtt gcagccacca
     4861 cgccgttggt accggatgac caggtgtgct gacgcacttc cgccaggaat ttaaagccct
     4921 taccggacgg aaacagcacc ttaaacgcat acaacgcgtc attgtcatag gcatcacgca
     4981 gggcgttctg ggcctgattc agataaaaat tacccgacat ggaaatctca gacgacgccc
     5041 ccagaccgtt gatgttctcc tgctctgtgg agcagagcgt ggtcacatca atatcctgtt
     5101 tctgaccggc ggtgaactgg acttccttga tggtgcagtc caggcgcaga tattccgcct
     5161 tatccatagt ttcagcagtc gccggggcag atgaaatcat cacctgcgtc agctgtgagc
     5221 gttcatacaa agcagacatt ctgcctcctg ataataaaaa acccgcacgc ggcggggtat
     5281 ggttttgtag aaaaaaagaa aaagtcacac cgtgacctga aactccaggg ttgcacggta
     5341 acagcggttt tccggaatat agtcctgcat ttcactgacg gatcccgggg ccagcagcat
     5401 tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc cggcataaac
     5461 gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg ctgacaccga
     5521 tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat ccagcagggg
     5581 gaccacatac gggtacacct gcccgccggc aagatgcgcc agatgaggat acaaatccgc
     5641 ctccgtcatc gtctcagtac ctcatcaatg gcccggttca tccgcgcaat cgccacctga
     5701 gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg tggcatattc
     5761 acggtcccca tttccacaaa ccgccagtag aaagcattgc gcgggttatc cgccttcatg
     5821 gtgttatcgc tgttaccggt gtccggatta acaccacgga tatggacacc ggattccatc
     5881 ccgccatcgc gggagcgccg ggaaaggatc accacattgc ggcgcagttt tcccctgcgt
     5941 accggtgccc gtgacaccac ttcttctttc agcacattcg cacccgcacg ggttgcctca
     6001 cgcagcaccc ggttattttc cgcaccactc agaagctgca aatcgcggct gatgtcctcc
     6061 agccccgaaa aatccagcag ggtttcgatc atttttcccc tcccagccga cagagaattt
     6121 ccagacgccc gccggtcgta tccggcacgg gcagcccgac aacgttcagg atccggtcac
     6181 gccatggacc actcagcaca tgaagtcgtg acgctgctgt gatttcccgg ccggactgac
     6241 cgcgcaccca gatgcggatt tccgcctgag ccatttccgc accggactgc atccgctccc
     6301 ggctgctcct gccccggata tccgcatgaa ttttcccgca tgacacccac tcttccgtca
     6361 tttctccggc agcattacgg gttaacaccg ggttcagaac acttatcatc tgtgtcagac
     6421 gacctgcaga tattgccatc cccccccctc ctcataacac cgtcggacaa cgcaaatcgt
     6481 aaatcagcac ggacacagaa aacggcagtt ccccctgcac gaggtcttcc cgctccgcaa
     6541 gatccggatt ccggtacaac atcccggtca ggcgcatggc agcccccttc atccgggtta
     6601 atgcctcgcc cgggatcagt tcaccgtcct cacggattac cttatcccgg ctgccctgaa
     6661 tgtaggccag cagcacagct gtagcctgac gaaccttgtc catcagcatg tcatcatccg
     6721 cgtcatggtc aacacgcaga tgagccttga tttcttccag tgtcagtaat gctgtcactt
     6781 tccacctcct gcatcccgcc ctcgttttgc agccagggtc cagcctgatg aatgagcttc
     6841 tccgggttta tcaccggtca tgctgttgca gtgccacaac gagccccccc acgtcaccgt
     6901 atcgccgggg tggtaggttt cgccggttct gaatacaccg cgatagagca tcaccggcag
     6961 ggaaaatgtt ttttccgtac actggccact gctctgccgg accaccacag tgaacgaccg
     7021 ttcgccggtc atgctgacgt caatatcggc caccccgtca accaggcatt cccatccccg
     7081 catcccgtgc gttttttcat acgcccgcca gagtccgccc tggtgtgtgg catacgtgcc
     7141 ccggggaaag gatttttgct catcaatcgc cgggaatatt tccagtgccg tagcatcacg
     7201 cccgtcctgt ggagccggca gggcactcac cgcatccaga accgccttct gcagaacttc
     7261 cggatcgtag tcacgaccgt cgcgcggaac ggggatatga ctcacggcat ccgtcaccat
     7321 ctgctcgagc atcggacgca catcatccgg tgtgatactt tttccatctg ccggtaccgg
     7381 aagttttccg acagcgtcat tcaccgccct cagcagcacc tccggatcat aatcacgacc
     7441 atcgcgcgga gcagggatat gactcactgc gtccttcacc atctgctcga tcatcgggcg
     7501 tacatcatcc gcagtgacac ttttaccgtc cgccggtacc ggtattttcg cgaccgcatc
     7561 attcaccgcc tgctgcagca cctccggatc ataatcacga ccatcgcgcg gtaccggaat
     7621 ggcccccacg gcgtcattca tcatcgcctg cagaacaggg cgcacctcat ccaccgtcac
     7681 atgcttctgt aacagagcag acagggaagc cagtttctct tcaaacgccc ccgactgtgc
     7741 ggccatcttc tcctcaaatg tgcgctgtaa atccgccagc accgtggcga attcttcacc
     7801 cagcgcacgg ataatggaca gttcacgctc tgtcattttc gcagtatccc cctgaacatc
     7861 gccttcaccg catcacgctc tgtttcgctt atggccttat taccgtcaga tgcgccgtca
     7921 ggcagctgtg atgaaactgt tttcccggtc gacgcgaacg gatcctcacg ggcatcacga
     7981 cgggacagcg cctccagact gtagttctgc tgctgaagat acagtgcatc accgccggcc
     8041 agaggcggca ggttctcccg tttacgggcc tcattgggcg tgagaagcgt atttttcacc
     8101 gcatccccca gcgttttcat gcgccgctca ctgtccattc tcagcagcgt ggtgacatca
     8161 aattctgtac tctcgttttc ccccgtttcc agcgcctcat ccagtaacag ttcaatggac
     8221 tcaatcagcg tctgcaggca ctgggaataa tactgctgct ccagcgcctc cacgttgtca
     8281 ctggaaggcg gttgtcccac gccaatcttg taggccggga cacggaacac cgaacagaca
     8341 atttcagcgg tcatcttcag ttgttccacc gtctgcgcat ccaccggtga aaacgtcgtg
     8401 gggttgtatt ttgccccgtt gctcagaatg gccgttttcc ccgcattttc gcctgtatac
     8461 ccgctgtccc agttgctctt cagttttttc gcattttctt ccgtaatact gccggggatc
     8521 tcaatcaccc cggacggcct gccgccattt ctgaaaaaag acgtcgaatt ttcctgaata
     8581 tgatgcccct gcgtggccgc cagcccggca gcatacaccg gcggcagccc cacaagcgga
     8641 tgaaaaaaac agttaaaccg gtcgtggatc acttccctgg caggcaccgt caccgcctca
     8701 gtgatcccgc agttccggtc cggtgtaatg cgatagaaca cctcgccgtc atccgccacc
     8761 agaggttcaa cccggctcca gtccagaata cgcagttctt tgatctgccc ccgggagtta
     8821 cggattttca acaccaccgt attgccgtga cgcagtttgg cgttcagcca cagttcaaaa
     8881 aactggatac gattctgctg tgcattggga cgacgacaga gacgggcaat atccccctgt
     8941 cgtttttcac ggcggatccc ctgtgtatcg gtctgcatca ggcgcagccg cattttggcg
     9001 atatcctggg atatcagcga aatgcaagaa aacaccgcgt gaaaggacaa aacggtttcc
     9061 ggatcggctt tcacaccctg ctgccaggca ccggcaaaag gctcagccac cgcctgaaac
     9121 aggctggtcc agcccacctc ttttacatca cgtcctgatt tctggttttt tcgggttcgc
     9181 cgcaaaaggt tccacattcg ccatgctccg catcacgttt ctttttctga cctgccggac
     9241 gtcgcactgt gatgtactcc gccttcccca ggcgaaccag cacctccgca cacggctgtg
     9301 ccacatcacg gatatccccg gcccgggcat catgcgtgcc ctgcagatac tggatttttg
     9361 ccatcagtta ctgcgggaag ctcgcgcctc ccgccctcct catcagactc agccgccgga
     9421 cgcagttccg tagttcacac cggtgatcac cgccactgcc gcggtacggc gacgacgcca
     9481 gttgatccag cgctccgcac ggatggccac gctgccggtc tggaacatgg agaccagctc
     9541 caccggtgac ggtgtgctgc tgtcgctggt cggttcagac tgcatctcca gtgacgcttc
     9601 acgggacata tccaccgcca caccgccgtc atcagccaga taaatatccg gcgcattcac
     9661 cagcaccagc tggtcaccca cgtactggga gacaatcacc ggcaggccct ggaaggtgcc
     9721 gccaagcagg gtcatgtccg gatattcctt ctgacccagc gcatttttac gcatggacag
     9781 cgccagggca tttgtgctgg acatcagcca gaccgcaccg gtgggctgca ggtttgctgc
     9841 cacaaactga ccaaacgcgg cttccgcatc cgtatccggg ttaccggttg atgccgtgcc
     9901 cttcacatca tgggtgatgg acgcagggga aacatcggcc accgccgctt ttttcgggtc
     9961 cacaaagtct gtgtccagac gcgccaccac cgcttccgcc agtgcattac ggaccagcgc
    10021 atcggcggca ggactggaaa aacggatcag ctcttccgtc agtaccgcaa tagccgccac
    10081 cttcgcatga ctgaaggtga tggattcaaa atcaaacttc gtcaggggtc tggccttacc
    10141 ctgccccacc cagccggcag caccaccgga cacctgggca tgcacgcgaa tattgaacgg
    10201 cacctgacga agtgccggga tcccgccatg accaaatcgc ccgataatgg tctgcggacg
    10261 cagataatca ataaagtcct gcgcatattc ctgatattca gacaggctgc ctgcccactg
    10321 tgggtccgtg gtggtccctg cccccaccgc cgacttcagg acatgatgca gacggctgtc
    10381 atccggatac tgacgacggg ccacttccag ggcttcagag cggacacctt tagccgcggc
    10441 cagtgatttg gcaaagcggg cgaaaccaat ccccttctcc agtttctgct caacacggat
    10501 caccggcgct gaagccaccg tggccacatt cccgttaccg gcctgtttca ccggctgtgc
    10561 cgtggcggcc ttactggttt ccagttcacg cagacgcttc aggtgcgcat ccacctgacg
    10621 gatttccgct gcggtgttgt cgtagtgctc ttcctcttcc acatccagtg tgcggccttc
    10681 ctctgcggct ttggtcatga cctcctcaag ggaggctgcc agcgccgcac gcttgttttc
    10741 aaaactttta atctgttcac cagtattcat tgctgacttt tccttatgaa aagaggttat
    10801 tgactgtgcc gaagcgccgg cagaagatgc gattttcacc accggtttcc ggttgccgga
    10861 cgcggcagaa aacgggcggt cgaaagattt aatggtctgg atggtgcatt ccgcattcgc
    10921 gggcacggtg acggcagaca cctccatcag ctcccagcgc agaaaatgca gtccgcctcc
    10981 gtccagataa gtgtattcat ggggccggaa gcccacagaa agccccctga ccagcccggt
    11041 cttaatggca gcccatgcct catccagccg ggcagccagt tgcgacggca tatccggtac
    11101 gggcttcacc agtgttgccg tgatttccag cccttccctg atccgacgca ccgtacactg
    11161 gcctacaggg cgggaatggt catgctgcca gagaaacggg atcgtactgc caaactccgc
    11221 cccctccggc tccaggatgt caccatcccg atccggagaa ggcgttgacg caatcccggt
    11281 gatcacccgt tcatcctcac tgaaggattt caccgtcagc agggaacagg cccgtttaag
    11341 agtcacatca gcctcctgaa aataaaaaaa accgccgcag cggttcatga tggttacagg
    11401 gtgagcaggg ttatatgaaa aaaacctcat acgctttctt tttcggttcc ggattcaggg
    11461 acatcaggga caccgcattg aagagcgcca tcagcgggtc aatttttccc cgtccgctgg
    11521 cctgtttggt aataaggatg gcgttacctt taggctccac ccgggcattg ccgacacacc
    11581 aggccatcag gggctggcca ccatgcacca gtaccccttc agccagtttg cgctcggtgg
    11641 ttttgatggc cccgcccagc ttccagccct ggcttatccc caccacaatt ccgtcgggga
    11701 tcccggcttc cgccagtgaa tccagaatct gccccacccc tgacgggtca ataccgatat
    11761 ggtccagtaa ctcagcctca tgaatgcgac gcacatattc cgccacttcc gccgtgtcat
    11821 ccccgacacg ccggacaatg gtcatatctc cacaggcaac aagatcctga aaccgggacg
    11881 cctcgctctt ccgtcggacc accgcggttt catgcgccca ggcatggccc cagcccagcc
    11941 attcgcgggt ctcccggtca cgcccaatca catacatccc cagcagatca tccagccctc
    12001 cgccgtcaat ccccaccgtc accacatcag cacgacgcag gatatcgtcc aggctgatac
    12061 aacggccctg ctcttcccag aaatcagccc ccgcccagcg gtcagagcgc agggcaagac
    12121 caatttccac attggcgtgt tttgacatga acccccggaa tgtctcttca ccggcttccc
    12181 gggctttacg gtactcccgg tacagaaagg cctcatccac tgaatagccg agattcggat
    12241 tgaccatggc gaggttttcc atcagcaggt gagccccgct ttccaccatt tcaggagggt
    12301 gttcaaatat caccggcaga aagtgcggat catgaatttt gccgtcgcgc acatcccggg
    12361 cgtactgcag tttctgtctg aacaccccgg caggcggttc attcgactgg gtggtcgtat
    12421 acaccacaaa cccttccggg cgggaggcaa ggccgcctat ggcttcacgt aacatgtcct
    12481 ccgccttgca ctgcttgcca aacagccaca actcatcaat cagcgtaccc acggacttga
    12541 taccggacac cgtattcgga tcggcagcca ccaccttcag ggtggtgtct gtcaccctgt
    12601 gggtgatggt ccggatatgg gtctgtacct ggcagaggtc atccagatca tcgtcacgtc
    12661 gtaccatatc ccgggcaggg ttgaaggcgt tggccgccac ctccacagtc ggggccagaa
    12721 tcgtgtaacc cgccgcctgc cgccagttca gtaacagtgc agtcatcatg atccccgcgg
    12781 ccagcgtgga cttcgagttt ttcttgggga taaggataaa aacttccttg atatggcgta
    12841 caccggtctg cgcatcgtag gagccaaaca gggccgccac caggtcaaac acccacggtg
    12901 cacaggactc cccgaacgtc gggctaccag gtgcatccac aatccgcagt tgtttaaaaa
    12961 tcgccagggc atgtgcggcc tcgtccggat aaatcggatc cggaataatc gacagcccct
    13021 ttttcaggcg ctctgcccag tccgggcagg ctgtgctcca tacaggtatc atccgttgcc
    13081 ctcattatcg ttattcacca ccagtcgggg tggcggtggc accgcaaaac ggttagccgc
    13141 ttttttcgcg gcatcacctt ttgccgattt tttcccggta tccccttttt tatggtgcgt
    13201 gaactgcgcc agacgccagg ccgcatccag tgccagtttc ggatcaatgc agaggttttc
    13261 caccaggatc cgccccatgg ctttcaccgg atcgggaaga ccatcctcca tatattcaat
    13321 accaggagac atcaccgcgg acggtggcat ctccggatta ttttcgtccg gctgaggtat
    13381 tgcagccgcc tcacggcgac ggggtttatc ctcctgctct gattttttct gccggtaaac
    13441 aggaacctca tccacctcca ccgtctcgca ttgtttacgg gctataaacg caagcacctc
    13501 cggatctttt gccagctgcg agcctttaac cctggcggtc ttcgccgaat aaccggcggc
    13561 aatggctgac gctgttttgt ttttcccgga catgagcgcc agcgcaaatt ttcgtttttg
    13621 cgttgtcagc acagcctcct cccgggtcca gaacgcactc agccgggtat ggttcagccc
    13681 atttttcccc ggcgtctcat gccgcaaatg ttaactgctg cctggttaac atttgctgaa
    13741 aaagcctgtt aacatttttt ccgcacaaca aactgaataa taaagataaa aaccgcaaaa
    13801 atgcccgggc agccagttaa catgttaact gccctgaaac gggaattttt tctctgcgtg
    13861 agagggggcg cggtgtccaa agcgatcgtt ttttacgccg gatgataccc cccccgggtc
    13921 gggttacagt ccgatgatgt cgtccgctct gtcactacct ccggacacct ccggcagcgt
    13981 cgggtccggc ataccacccg ccgcttcacg agcagacttt tgtcgatggc attcggtaca
    14041 gagcgtccag agattcgtct cctcattacc accaccgaac tgaagtgcaa ttcggtgatc
    14101 gagttcactg tcacagaggt caaccacacg accacagata cagcactgcc cggcgtccct
    14161 cagccagata tgacgcttga gggaaacacg tgcactgcca ctgacacgac gctgttcccc
    14221 cttcagaata ttcacccgtc gggtattcag tgttttgatt ctgctctgga gtgtacgaag
    14281 ctcagccatg taaaatcccc gtcatatggc aatcagtaaa ggaaataaat atgtcatcga
    14341 aaaaccggac ccgcagaacc acaacccgca atatccgttt ccccaatcac ataattgaac
    14401 aaatcaacat agcccttgag cataaagggt ccggtaactt ttcagcgtgg gttattgaag
    14461 cctgcaggag aaggctggca acagatgcaa cgcatctgcg cccgaccagc atgacaaata
    14521 acgagaaatg aacgttcggt ttcttccacc atcgcaccgg acaggcgact atgaggggac
    14581 aacgccgcgc tccgttaacg cggtaaaccc cggtgtgtat cgtttttgat tatccccgca
    14641 cactcgcgca gaggagtctc cctgtcgggc tgcggtctct gttaatgcag gaatacggcg
    14701 acaataccgc gcatggataa taaggtcgct caacacactg gctgtaatgc agcggatacc
    14761 atgcggcatt tagcggcatt catcgtacac tccacggtta gctcttcatt cgtggcattc
    14821 acctgaaagg tccgggagtg taattgcgta catttaccac tgaacgaacc ttcaacaaga
    14881 acacgaccac gctgcaaaat acggaacgga attgttccct gaaaaggttc tacggttacc
    14941 cgtaatttct tcatgtatcc tccggataat aaaaagccag cttagtgcac tgagtgcgga
    15001 tatattcctg cgccccttcc agctgcttct gcattgtcat cagccgttct ctgaggatga
    15061 aataatcccg ttcagcggtg tctgccagtt gggggccggt tgcattatcc acgcgggcgg
    15121 tgccggtggc ttcacgcacg ggacctggac aggtggcgtt gatacgcagg cgcttacgac
    15181 cagcggcaac gtcagcgcga agagtttcat tttcagctct cgcatcggct aattccctcg
    15241 agtattttgc atcgagcgca gcaacatcac gctgacgcat ctgcatgtca gtaattgccg
    15301 cgttcgccag cttcagttct ctgacatttt tgtcgcgctg ggctttgtag gttatggcgt
    15361 tatcacggta atgattcagc cccagactaa gcgcaccaca ggccaccagc agggcaatga
    15421 tgaccacgca cagtacgcgg ttcatttcac caccagcgta tctgaccgat gaaataaccg
    15481 gaggccataa tcacaaacac cagccagata agaatgaact tccaggtgga taatttttca
    15541 gccatcactc gaatctcccg aatcagtttg ctaaaatcaa acacactttc tcctttgact
    15601 tttccagagt caggaaacac aaaaccccgc ttgcagccaa caaacggggt ttttactttt
    15661 attcacttag tttttgtcag ttcgcaggat ttcgtgttat ccgtccgtgt gagcaaaccg
    15721 catttttcag caaaatattc tgcttatctg tcaattcccc agcacgccag cgcactctcc
    15781 tggtcgcgac gggatacctg accgtaacag ttgtttgaac gaatacggca gtctctgcca
    15841 ccgtccttaa tccaccagcg aatcgcttcg caggcacctt ttcgatcacc tgcattaatt
    15901 cgtctgtaaa acgtcgacgg gaaacactta ccgggaccaa tgttgtacgg acagaatgac
    15961 gcgatccccg ctttctgggg ttcggtcagt ggcactctga tgtttttctc cacccatgcc
    16021 agcgccttat cacgctcaat ggcgttaacc cggtcgcatt ttgccttcga caacttcatg
    16081 cctggaacaa caggtttacc atccaccatg atggcaccac ggcagatggt ccagatcccc
    16141 gcaccatcac ggtatgccgt ggtgtggtta ccttcctttt catccagaaa ctggtcgaga
    16201 atgtcaggcg caggcgcacc agcggcaatc agcgccagaa cggcagccga caggccgtat
    16261 ctgatttttg cgttcatgga tatttatcag gatttatcgg tttctgccca cggacaggtt
    16321 tatctgttcc ggtcagtgaa ttaaggttgt gattccggtg gagtcttcag agaaccagta
    16381 attcttcccg gtagatttcc tttgtaggtt atccatacat tctgcgcctc taaaatcacg
    16441 gggcgctttt ccggcaacgg ttcgttccct tcacataacc cggcagcaac atccatgaaa
    16501 aactgcttcg cctgcttttt cgcctcagct tcgtaaagct ccagcgtggc accttcagta
    16561 cggtcaagac taatcgccac atctggcaac aacagtgacg gatacccacc aacttccagt
    16621 gccacagtaa cagtaatctt atccgggtaa ttatttatcc ctttaacaac cagttcgtat
    16681 tttttcttca tcgctttact ctccccgcgc cgccttacgc cggtcctct
//
LOCUS       DAZFRG010000103        16697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFRG010000103 DAZFRG010000000
VERSION     DAZFRG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.103"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(218..754)
                     /gene="yghD"
                     /locus_tag="ACHKUC_002624"
     CDS             complement(218..754)
                     /gene="yghD"
                     /locus_tag="ACHKUC_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO6618864.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAA
                     VFLFCAVYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIIAARKSGTTTQAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVLRLEFGRG"
     gene            complement(756..1934)
                     /gene="gspL"
                     /locus_tag="ACHKUC_002625"
     CDS             complement(756..1934)
                     /gene="gspL"
                     /locus_tag="ACHKUC_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6618865.1"
                     /translation="MSSILEIFFPLCDSDTVRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPFSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1931..2908)
                     /gene="gspK"
                     /locus_tag="ACHKUC_002626"
     CDS             complement(1931..2908)
                     /gene="gspK"
                     /locus_tag="ACHKUC_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO6618866.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHQAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2905..3510)
                     /gene="gspJ"
                     /locus_tag="ACHKUC_002627"
     CDS             complement(2905..3510)
                     /gene="gspJ"
                     /locus_tag="ACHKUC_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6618867.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMALQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3507..3878)
                     /gene="gspI"
                     /locus_tag="ACHKUC_002628"
     CDS             complement(3507..3878)
                     /gene="gspI"
                     /locus_tag="ACHKUC_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6618868.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3875..4438)
                     /gene="gspH"
                     /locus_tag="ACHKUC_002629"
     CDS             complement(3875..4438)
                     /gene="gspH"
                     /locus_tag="ACHKUC_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6618869.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(4442..4897)
                     /gene="gspG"
                     /locus_tag="ACHKUC_002630"
     CDS             complement(4442..4897)
                     /gene="gspG"
                     /locus_tag="ACHKUC_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6618870.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4914..6137)
                     /gene="gspF"
                     /locus_tag="ACHKUC_002631"
     CDS             complement(4914..6137)
                     /gene="gspF"
                     /locus_tag="ACHKUC_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6618871.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQHLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6137..7630)
                     /gene="gspE"
                     /locus_tag="ACHKUC_002632"
     CDS             complement(6137..7630)
                     /gene="gspE"
                     /locus_tag="ACHKUC_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO6618872.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7630..9690)
                     /gene="gspD"
                     /locus_tag="ACHKUC_002633"
     CDS             complement(7630..9690)
                     /gene="gspD"
                     /locus_tag="ACHKUC_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO6618873.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLALVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9720..10679)
                     /gene="gspC"
                     /locus_tag="ACHKUC_002634"
     CDS             complement(9720..10679)
                     /gene="gspC"
                     /locus_tag="ACHKUC_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6618874.1"
                     /translation="MARVVFRDARIYLIQLLTKIRHTLSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEKRPTKAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(10697..11107)
                     /gene="gspS2"
                     /locus_tag="ACHKUC_002635"
     CDS             complement(10697..11107)
                     /gene="gspS2"
                     /locus_tag="ACHKUC_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO6618875.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGAQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11173..11982)
                     /gene="pppA"
                     /locus_tag="ACHKUC_002636"
     CDS             complement(11173..11982)
                     /gene="pppA"
                     /locus_tag="ACHKUC_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO6618876.1"
                     /translation="MLFDVFQQYSAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKINLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12112..16668)
                     /gene="sslE"
                     /locus_tag="ACHKUC_002637"
     CDS             complement(12112..16668)
                     /gene="sslE"
                     /locus_tag="ACHKUC_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO6618877.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRVTG
                     ATCNGESSDGFTFTPGEDVTCVAGNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELA
                     ASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGR
                     LVDSMGNGVVGVNYYTSSGRGVTGENGKFNFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSQAGKNDEREVPDVVRKVFAEYPNVINEIINLSLSNGEA
                     LSEGDQTFERTNEFLEQFESGQAKEIDTAICDSLGGCNSQRWFSLTARNVNEGQIQGV
                     INKLWGVDKDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPH
                     YNSILRCPNGYSWEGGVDKNGQCTRNSDSNDMKHFMQNVLRYLSDDKWTPDAKASMTV
                     GTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYV
                     TQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTG
                     EVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKIFE
                     KFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADL
                     GTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNVSVWLWNKIEYRYENDKDDELGF
                     KTFTEFLNCYANDAYTGGTQCSDELKKSLVDNNMIYGEKSVNKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIESSASVPVTVTVALADDLTGREKHEVALNRPPKVTKTYDLKANDKVTFK
                     VPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDAFVYT
                     TPKKNLEASNFTGGVAEFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKQWYPEGDLPEFYSDREGMKGWNLFQLMHRKARGDDVGKTKFGERNYCAES
                     NGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMNFEGGVSQSAYETLAA
                     LNLPKPQQGPETINQVTEHKMSAE"
BASE COUNT         3680 a         4776 c         4208 g         4033 t
ORIGIN      
        1 caaaacactt aagcatcttt acagtcttag aaaggtgtcg attaacgaag gctacatggt
       61 gaaaaacagg ttccttcagt gttaccgaac attccgtggt ggtctggagg gagataaatc
      121 ttgctacatc ggaactcagc ccggtgagct ggtttgacag ttccgcactc accattgggc
      181 taagggttat caggtggggt taaggaaatg gcaataccta cccccgtcca aactccagtc
      241 gcagcacatt taccatccct ggcttctcag cagcactgac atcaatttgt gtcacccgca
      301 gcgcatattt ttcatccagt gcgtttaacc atttcagaag gtcattaaac accacaggtt
      361 ctatccagac ctgaatattc tccccacgct cggctatccg cttgatgacc accgagtgcg
      421 cggaagcgct gtcactgatg acccgcgata cctgtgcttg cgttgtcgtg ccggattttc
      481 gcgccgcaat aatatccggc gcagtgctct tcagtcgcgc gttcatcgcc accagctgct
      541 gcaacatcgt ctcctgttgt tctatccgct cgctcaacgg ctgccagatg aggacgtaat
      601 atacggcgca aaacaggaac acggccgcgg ccagtaacat gcctttttca cgcggcgaac
      661 gccccgccag gtgctgtgcc agccagtgtt cgccacggct taactggcgt tcacgccatt
      721 gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa cgttacgccg
      781 ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata atctgccgcc
      841 agtgcgctac gcagtttatc gaagctggca aagttcgcag cccgtagctg gaggtgaagc
      901 gtctggcgtt tttgatcaaa ggtgaaacca cgcatttcga tgtcggtaag tgacgctgat
      961 ttcagggtac tggcgatcgc tgacaattct gcgagcagcc gggtatcgtc ggcctgtggg
     1021 cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg cttctgctcc
     1081 gggaatagcg ttaagaactg tttctccgcc tgggtgcggc tttgcgccac ctgttcgctg
     1141 acgctccata acgtcacgcc ccgttccact gccagcgcaa ccagaatcag caatatcggc
     1201 agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg ctgccacggt
     1261 ccagtcagca ggttcccttc cggttcgcca taagtggtaa tggcgggcag agccgtcact
     1321 gtcaggcgtt cggcgtctgc accagcccat gcagacagtt cttccggtgc aatgccgacc
     1381 acggttagtg aaaacggtaa atcctgctca ttgagctgtg cgcggaacat gaccggagcc
     1441 agcgcccgcc cggcgctcca tccccggcat tcatcgatgc ggcagataac ccgttgcgca
     1501 tcgccagcca tatagccaca aggaatggac atccagtctg gcgcgacggt agcgcgggtg
     1561 atgccgtttg cctgcaacca ttgcgcaatg ttacgcatat gctgttggtg aatcactgct
     1621 acggttgcca gttgctggtc gattttcaac ggggcgaaat gcagttcatc gatatcctga
     1681 ttcagctctt cttccagcaa ggcgggcaga atagtcggta tctgcttgcg ggcgacatca
     1741 ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg aatcgcatcg
     1801 gtttgcagcc attgctggag acgttcgtca gcaacgtcag gccagatacc gtgttccaca
     1861 tcgggtgtac ggcgctgcca gcggacggtg tcggagtcgc aaagcgggaa aaaaatctca
     1921 aggatggaac tcactcactt tctcctgtct gatgccagag aacagaaaag tgttgtgggc
     1981 ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg atatctgaac
     2041 gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt ttagtacgct
     2101 catcgacgtc tgcgagtagc ggctgtgcaa gaaactgatc gacatcttcc cagcccttcg
     2161 ccggacgttg ttgtaataac gcccgcgcct gtacagggct taaccacggg tcaaacagcg
     2221 cctcaagaat cacactttgc gtgacatcca gggtgttgat gttgatttgc tggcgggtca
     2281 tcggcagcgc acagaccagc ggtttcagtt tttgataaag tccggcgtcc attccctgca
     2341 ccacgcgcat ctcactgata tcggccagcg gttgattagc ggcgtaaaac ggcaccgaac
     2401 gggcgagata ctcgctgtct tcacgaccca gacgcgtctg cacgctgcgg tcttcgtcaa
     2461 taaactccca caggctttcg gctatcagtt cggcacgata agcaggcaca tcgaggcgcg
     2521 agatcagggc aatcagttgt tgtaccgcga gcggacgcga cgccgtcgtc ggctgagcga
     2581 gggcattcag gttaaagcag gcctgtgcgt cacgcagagt gacggcgatt tgcccctgcg
     2641 gcagtgggaa aaaacgaggg ccagaagccc acggctgtgc ctgatgcaca cgcttttcat
     2701 ttttcaggct cagactgagt gcgctcaacg ccaggctttc cgcactggcg ctgtaccaca
     2761 gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc atccgcccgg
     2821 aaagcgtgat ggtcaccagc atcataaccg ccagcaatac cagcaccacg accagtgcca
     2881 ttccgcgttt tggtggtgag gtgatcatga taattgcggc ccgcgtaaca accagatgcg
     2941 ttcaatttcg ccccattgcg gcgaatgcag ggttatgcgt actgccacag gaatcgcctg
     3001 cactgatgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac ggagcgaatc
     3061 cgccgggatt aatttttgcg ttgttggctt cacgctgcct gccgcatcgg tcagtggcca
     3121 ggctaaccgt tcgagataac caccatgaat gcggtaaccg acggtgagca gattactgcg
     3181 cggcagacgc atcaacggat taaccacgcc gccgcgcaca aaacgcatcc cttcactctc
     3241 agacgccagc acgccagcgc ccgccagtaa cgctggttca cgctggccct gatcgcctct
     3301 taccggacgc ggcatcattt gtgtcagatc gtggttcaaa aaactcatcg tttgctgcat
     3361 gaggtttagt ttttgatcgt gtccggcgac ggcgctattc acgcgtgtaa cgccgtttgt
     3421 cacctgcagc gccatcagtg ccagtgaggc aaaaatggca attgccacca gcatttccag
     3481 taacgtgaaa ccagcgcaag tccttctcac tgttggcctc ccacggcgct aaaccaggcg
     3541 cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat atccactgcc
     3601 tggagcagcg cgttgcctgt actcagcggt gtggttcgcc agtaccagcg gcttcctgcc
     3661 atatcactct cgccctggtg cctgaccgct cgttgttcac gggtcatcag gtaaagcagt
     3721 gcggtctggt tttcagctac ccagcccgct accgtttttt cttcaaggat ttgctgatta
     3781 ctcagcgcgc cgctggcgat ctgtaacacc gccatggcgg ccaacgcaaa aatcgccagc
     3841 gcgagcatta cttccagcaa ggtaaatcca cgcttcatgg catcccctca tcacattgat
     3901 tgagggataa cacgccatcg tgcgccagct ttaccgccca acacgcgttt tgtgccgacg
     3961 cgtaaaaacg cagtgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg atctgcggtg
     4021 tcttcttttt cgcctctttt tgcagctcca gttcaatatc gtgcagcgtc aggcgacgac
     4081 gctgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt aacagcatct
     4141 gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg ggcaaccatt
     4201 gcccgtgacg acgctgcata aactggtaac ctggcgggtc gattagcaca ccgagtgttt
     4261 gcccttcgat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa tcctgcgccg
     4321 cttttttcgc aggcggctct gaagcggtcg caaacgtctg taccacgccc gcactggcaa
     4381 ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt tcaggcactg
     4441 attactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgccc tccccgtttt
     4501 cctgaccatc tgctcccagg gtataaacat caaacagccc tttttcaccc gggctgagat
     4561 actgataatc attgccccac ggatcctttg gcagtcgttt aatgtatcca ccggtacggt
     4621 agttacggga atccgccata ttggccggtt gctggatcag cgcctccagc ccctgttcgg
     4681 tagtcggata acgcccgtta tccagtcggt acatatccag cgcattctcc agcgccacga
     4741 tatcgctgat ggctttttgc cgatcggctt tctctttgtt gcccaacagg ttaggcacca
     4801 ccagacttgc taggacgcca agaataacaa tcaccaccat cacttccagc agggtaaaac
     4861 ctgcccgtgg tttttgtgtg cgggataacg aattcatgtg ataactccgt aaattacatt
     4921 ccaaccatat tgttcagttg cagcatcggt tcgaggatgg cgatgacgat aaacagcacc
     4981 acgcccgcca tcatcaccac cagcgccggc tcaaacagcc ctaacgccag ccccacctgg
     5041 gtatcaaatt cccgttcctg gttgaccgcg gcctgttcaa gcatggtttc cagttcaccg
     5101 ctctgttcgc cggaggcgat catgtacagc atcatcggcg ggaacaggcg caactccgcc
     5161 agcgcggcgc gcaggctgct tccttcgcgg acgcgatctg ccgccagcag cagttgttgc
     5221 tcgacataac gatttgccga cacggcggcg gcggtctgaa tgccttccag cagcggaaca
     5281 ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag tccgcgcgca
     5341 acacgccccg tcacgggcaa acgcagcaag gttttatccc agcgcaggcg catcgcagga
     5401 tttttgagta gccgttgccc cagcaccaga agccccagca aacccgccag ccagtacacg
     5461 ccgctggtct gtaacgtgtc gctcatggcg atgagcattc gggtggaggc aggcagcgcg
     5521 tgtccgagat gatcaaactg ttcgataatt ttcggcacta ctgccgtcag caaaatagtg
     5581 accacgcccg ttgccaccac cagcagtacc agcgggtaga gcatggcctg cagcaggcgt
     5641 gatttcagat gctgccgctg ttcggtgtaa tccgccaggc gattgagcac cacgtcgaga
     5701 tgtccggatt tttctccggc agcgaccatc gaacaaaaca gggagtcaaa gacgcgggga
     5761 tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg cagcgccatt
     5821 ccgaggcttt ttacatgcag tttttcactt tgctcactga ccgcctgtaa gcaggtttcc
     5881 agcggcattg ctgcctgcac cagcgttgcc agttggcgtg tgaacagcgc caaatctgcc
     5941 gccgccacgc gacgatgtgc atgccgccga cgctgcaaca ttccccctgt cgaagcattc
     6001 agccgggctt caatgtgcac ggggataagc tctttaccac gcaacaattg acgggcatga
     6061 cgcgcggaat ccgcctcaat cattcctttg gttttgcgac cattacgctc cagcgcctga
     6121 tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg agagaggttt
     6181 ctccggcgag cactttctca atgccgttgc tgcggatacc cgcagagtgt tgccggacat
     6241 aacgttccag ctccagttcc ccggcctgac ggtggatcaa atcacgtaat gtggcgtcca
     6301 ccacgatcag ctcatggatg gcagtacgtc cgcgaaaacc tttgtgatta caggcgggac
     6361 agccctgcgg atggtacagc gtgacggttc gcgcatcggt aatccccagc aggcgttttt
     6421 cttcgtcggt ggcaggcgcg gactggcggc aatcgggaca cagcgtgcga accagtcgct
     6481 gcgccatcac gcccgtcaga ctggaagaga gcaggaaagg ctcaacgccc atatcctgca
     6541 aacgcgtgat cgcccccacc gccgtgttgg tatgcagcgt ggaaagtacc aggtgtccgg
     6601 tcagtgaagc ctgaacagcg atttctgcgg tttcggtatc gcggatttca ccgaccatca
     6661 ccacatccgg gtcctgacgc aaaatcgcgc gcaggccacg ggcgaaggtc atgccgacgc
     6721 gggtgttaac ctgcgtctga ccgatccctt caatcatgta ttcgataggg tcttcaaccg
     6781 tgagaatgtt acgcgagtgg ttgttcagct cctgcaatcc agcgtacagc gtggtgcttt
     6841 tgccggaacc ggtcggcccc gtcaccagaa aaatgccgtg cggtttgtgt aacagctggc
     6901 gcaactgcgc agtcagttcg agacttaaac ccagacgctc cagcgtcagg cgagcctggt
     6961 ttttgtccag cagtcgcagc accacccgtt ccccccaggc ggaaggcatg gtggagacac
     7021 gcacgtcaat cgcccgaccg cccagcagca gcgcaatgcg cccatcctgc ggtacgcgtt
     7081 tttcggcgat atccagccgt gccatcacct taatacgcga taccagcagc gaggccagtt
     7141 tgcgccccgg acgcagcatt tcatgtagtg tgccgtcaac acgaaaacgg atcaccagac
     7201 tcttttcaaa cgtctcgatg tggatatccg aagcgccttc tttaatcgcc tctgccaaca
     7261 tggcgttgat cagtttgatg attggcgcat cgtcgtcact ttccagcaga tcttccgttt
     7321 ccggtagttc ttctgcgaga gtaaaaaagt cctcagcaga accgagatct tccatcagct
     7381 ggcgggcctc ggaagagtcc cgctgccaga ccgcattcag ccgctgttca aattcggcct
     7441 cgtcgatttg cctcagcgta aaagacgcgt tcagcccccg ctgcagctcc tgcaaaacag
     7501 agagcgacaa cggatgaaca tggaggatct ccagcgacgc ttcgcaccat gccaccaggc
     7561 taaaccgacg gctgaaactg tagggcagac gcacggtgtt agcggtggtt tcctgtgcta
     7621 caggcaccat taacgcgttc tcccggcatt aaggaacgcg cgaacttctg gcggtagagc
     7681 ctgattttgc gctggcagta ccggctgcgc ggtgtgcggc atcaggctca agccttgctc
     7741 atcgcgatag atctgttcgg cgcgcatata gttatatttg cgctgcgaca cgccgtctgc
     7801 cgccataccg tcacgcagaa tggtcgggcg gataaatacc atcaggttac gtttttcttt
     7861 tttatccgcc gtcgatttaa acaggttacc aatcaatggg atatcgccaa gcagcggcac
     7921 tttcgccacg ctttctcccg cctgatcgtc catcagaccg ccaagcacga tcagctcacc
     7981 atcgtttgcc agcacggtgg ttttcagttt gcgctcgcca aacacgacgt cgagactggt
     8041 ctgtccttcc accttcgaca cttcctgctc aatcaccatc tgtaccgcgt ttccttcgtt
     8101 aatctgcggc gtgactttca gcatgatgcc gacttttttc ctctctaccg tattgaaagg
     8161 attgctgtta ttagagccaa cagtagagcc ggttaatacc ggaacgtcct ggcccaccat
     8221 gaagaaggct tcctggttgt ccagcgtggt gatactcggc gtggagagca cgttcgagct
     8281 ggagtcgttt ttgaccgcct gtaccagcgc catccagtcg cctttcacca cgccaaccgc
     8341 cgtaccgcta aagccagaaa gaagctgagc gagcgtggag agatcgccgt tagtatccgg
     8401 gttaatggtg gtagcgccgt tttcactgat caccgtggag cctttctgcg gttttgcctg
     8461 agaaatggct gcacccagcg tgccaatagg gatctgcgta ccgttagcaa actgcattaa
     8521 tccggcatct ttcgacgccc actgcacgcc gaaattgata ttgctacctt cggcaacttc
     8581 cacgatcaac gcctcgacgt gcacctgagc acggcgaata tccagttgtt caatcacgct
     8641 ttgcagcgac tgcatgatgt cctgcggcgc ggtaacaatc agggcattac tgtgtttgct
     8701 ggcggcgatg gagacaacct cacgcccgct accgacggtg ccttccgcct cttctttagc
     8761 cgccgtgagc gtgccgctga cctgcttcag tacatcgacc agatcttcgg ctttgctgta
     8821 tttgagatag aaaacctggc tgttgccgct gcgttccatt tctgagtcca gccgacggat
     8881 caggcggcgc attttgtccc gcgtggccgg gtcaccactg acaatcacac tgttggtgcg
     8941 ttcgtcggcg acaatttgag atttcagcgt cgcaggctgg ttctcgccgc tgtttttcgt
     9001 caggctttcc agcacgcggg cgatttccga agccgaggcg ttatccagcg ggatcacctc
     9061 ttcggtgcga ttacccgcat gatccacgcg ctggatcact tccgtcaggc gctccacaac
     9121 tgaggcgcgt ccggtaagca taatcacgtt ggagggatcg taattaacaa cgttgcctga
     9181 gcctgcgctg tcgatcatct ggcgcagaat cggtgccagc tcgcgtaccg acacattacg
     9241 taccggcacg actttggtga ccatttcatc gcccgcgtag ttgtcgctgc cttcaccaac
     9301 cagcggcagc ggctcgactt tcgcggcact ggatttcacc accttcagca cgtcgttttc
     9361 catcggtacg acggcatacc cctgtgcttc cagcaggtta aggaatagct ggtaatactg
     9421 gcgttcattg agcggggtca tggtgcgaat actcactttc ccctgaacgc ccggccccat
     9481 aatgatggtt ttattaaggt tagcgccgac ggtttcgatg aacgatttca ggtcggtatc
     9541 tttaaaatta gcggtgaaag tggcttcttc cgcccagacc ggtgaactgc ataatgccgc
     9601 tgccagcacc agcgccagta aacgcttttt tgttttgagg ccagttgtct tcttacgcca
     9661 gaccgacaac gtcatatcac gccaaaacac gatgaatgat tctcctggat taaatgcggt
     9721 tagcgcagcg cgatggaaat gtcgtagcgc gcacccttgc gtaaaaccgt aagttgaatg
     9781 gaatccattg aaggtaactg ccgcatcaga gcaatcattg ctcgtggatc agtgaaatcc
     9841 tgctgattta gcgcaatggc gatatcgcct tccttaaaac cgctggcatc gaacagagaa
     9901 cgatctgccc ccggtttcac tgcataaccg acaatccctt ccttacgcac aggcgtaagc
     9961 tggatatagt taaaaatttt ctgcggatct ttcgccagtg cctgacgcac ggcagccggg
    10021 atctcaactg gcgcactggc agcaggctca gcaacagctt gctttgcttc gtcgctgacg
    10081 gcttttttgc tggtcacggc tttggtggga cgcttctctt ctgccagact cagacgttcc
    10141 attttcccct gatagcgcag catcacatgg tcacgattaa tttcctcaat caccgcgttg
    10201 tgagagccaa gccgttcgcc ctgtaaatag acctgctgtt tgccgccttc ttcaatcacc
    10261 gcgccgggtc tggcaccaaa ggcgatcccg cgcagcacca cattaagacg cgtttctgcc
    10321 acaggcgcag gttcgggttg ttttaccggt gcggcgacgg gttgatattt gccaaaccag
    10381 ttttgttggc tgactaattg cacgtcattt ttatcgaacg gttttgcatc cgcacggagc
    10441 ggtttattcg ccgatgagga aaccgccgta tattccgcag aaaaggagaa atagcgccag
    10501 agtgaatacg ccatttttgc agaaataata agcagcatga gccagaacat cccgcgcacc
    10561 atttttcgca gatgctcttt gtctgtatta agagattgtc tctggctaag agtgtgacga
    10621 atttttgtca gcaactgaat gagataaatt cgtgcgtcac gaaaaacaac ccgcgccaat
    10681 ttatatctac ccgacgttat gctttgacta ttccacaggt ggtacgatcc agtttccgct
    10741 gatactggtt tcctgtacgt gtatcattaa tcgttatgct gtaattaatt ccctcggtaa
    10801 tcattaattt caccgtcggg tcagcgcaca tttgtcgttg atagctggtt aaaaaggcat
    10861 caggtgtctg cgtggtctga gcacccgctt cgctgataat ggtcattttt accgtcgtgc
    10921 cgctactttg cgccagcact aaggtatatc ccgcagattt aatcggcagg ttttggctga
    10981 tattttgcgc ctgttttttc gccagtaaac tggcattttc attatggccg gcacagccac
    11041 ttaataatcc tgtaacgctc aacaatagcg atattaatac cctccctggc atttgtttta
    11101 tcgacatggt aaataatctc taaggttatt aataagagtt aaaatgtcac tttgataatg
    11161 acgtggttat cattaaaaca atgcctgtag ataaagtgtt gctataccgc ccagacttaa
    11221 acacggtcca aaaggcagtg tggttgatcc tctttttgta ataacggcat atatcaggcc
    11281 gcagcatgat gcgattaaag caacattggg tagcgacaac ggccccaccc agccacctaa
    11341 cgcagcgaaa agtaatacat cgcccatgcc taatgcttct ttacgcagaa ctattccggc
    11401 tatccagcgc agggagtaaa aagtgataaa ccccaccagg acgccggtaa ctgcgtcttg
    11461 tagcgttaac ggactctgtt gcgcccatgc cgcaatcagc ccggtccaca atacgccctg
    11521 agtaaaaaca tcgggcagcc attggttatc gaggtcaatg acgctcgcgg caatcagcca
    11581 ggcggataat atcatcaccg ccagccccca tccactttct ggccacacca gactcgccag
    11641 caaaaaagcg agtgccgtca ataactccac cagcggataa cgcttgctga ttttcgcctg
    11701 acagtcgcgg cagcgccctt tgagcatcaa ccatgagagc agcggaatat tgtcacgaac
    11761 gcggatggtc tgctggcaat gcggacagtg cgagcgtggc agcgccaggt ttatttttga
    11821 ctgcgtactc ggcatttcac cgtgaaactc cgccatttgt tggcgcagca tgatggggta
    11881 acgccaaatc accacattca aaaaactgcc gatgatcaat cctccgacgg ttgccagtat
    11941 gggcatcccc gcggagtatt gctgaaaaac atcaaaaagc atggttaaag gttgtttgtt
    12001 gtaacttgcc ggatgcgggc ctgcgggtgt atgccatatg gctttcctcc aggcccgatg
    12061 cgccttattt catgccggat gcggcgcgag cgccttatcc ggcatacagg cttactcagc
    12121 tgacatctta tgctcggtaa cctgattaat ggtttccggc ccttgctgcg gtttcggcag
    12181 attaagcgcc gccagcgtct cgtaagccga ctggctcaca ccgccctcga agttcatctc
    12241 gctcgctccc ggtaactggt aagcattcgc gcccggattc catttcttaa agaactccga
    12301 aagatccgtc tgagcgaccc aggaggcaca cagcatcagc ttgtcggcag cgttaccgtt
    12361 ggattcggca cagtaatttc tttcgccaaa cttggttttg ccaacatcat cgccgcgtgc
    12421 tttacggtgc atcaactgga acaggttcca gcctttcatc ccttcacgat cgctgtagaa
    12481 ctcaggcagg tcaccttctg gataccactg tttgatatca aagtttttct ctgcccactc
    12541 cttcagctgc gcgtacatca gcagacggtc gcccgcaccg ccgcgcgccc atgcctgacc
    12601 gttgctctcc tccagatatt ccggcgcgac agtaatatcg tcagcgacac ggttcatctt
    12661 gccgagatag cgatcctgca tgtacagcgc cagaacgttg ttcgctactt cagttgcgcc
    12721 aggaacagtc agcggcgttt cggcggcgtt gtgccctact tcgtgccaga tcaaccagtc
    12781 gttcagcggc gtcgtcggca gcgtggtgct gttcgtcgag aagctgctgt tcattaccgg
    12841 ataacccgag tgcgcatcac cgatggagat ctgcacatcg ttggtgaaac gatgcttgtg
    12901 ccccgtcaag tttttatagg taaacatccg gtgcttaccg tcttcatcat tacgaccgta
    12961 gaagtcattc atcgagctgg caaaggtatc cagatcttta gcgaattctg ctacaccacc
    13021 agtgaaattg ctggcctcaa ggttcttctt cggcgtggta taaacgaaag cgtctgattc
    13081 cagctcgccc agcggcgcag gggagttcag agcgtttttc cattcgccgt ctttatagaa
    13141 cggcgctttt accacaccag taaaggtgaa ttcggctgac tcattctttg ggctgttgcc
    13201 cttgatataa atcagaccac cgtaaggcac cttgaaggtc accttatcat tggctttcag
    13261 atcataggtt ttcgtcactt ttggcggacg gttcagcgcg acttcatgct tctcgcgtcc
    13321 ggtcagatcg tcggccagcg ccacggtgac agtcacagga actgatgcag aagactcaat
    13381 ggtgacctct ttctgagccg gagcccacag gccagtagac tgcatgttac ccgcaaacca
    13441 tttggtcgga ttggagtaca ggctgatggt ttcagtaacc ttctcacctt ctgccgatac
    13501 cgctcccgga tacttctcga catcaacttt gatgttcaga tcccaccagg aacgacccag
    13561 catcaggcgc gtcagcggtt tttccatata gttgagcgga tagctcgggt tcatcatgcc
    13621 cgctttatta acgctcttct cgccgtagat catgttgtta tcgaccagcg attttttcag
    13681 ctcatcagaa cactgcgtgc cgccagtata ggcatcgttg gcgtagcagt tcaggaactc
    13741 ggtgaacgtt ttaaagccca gctcgtcatc cttgtcgttt tcatagcgat attcaatttt
    13801 attccacagc cagaccgaca cgttctggta cagacgttcc agatcgacgc tgctcagacg
    13861 ctcacctttg cgaccattgg tccggaagta gagctcatgc tgatacagac gctgaatgtt
    13921 ggtgcctaaa tccgcagcct gcaccatcgc ttttgccgtg tcggcgttaa ggcttagttg
    13981 cgtatactgt ggaacataca tgccaccagt aaccggaacc cccgtgccag gacgatattc
    14041 cagacagttg acctcgtagt ggtaagttgg gtccttacac tcctttaatc caggaaactt
    14101 ctcaaagatt tttgccttcg cagccttcag agaatcctct gttttatgat cggcctcatc
    14161 aataaaggca taacgcgttt cctgtttgcc atctacatct tccagccagc tggcaacttc
    14221 cagcttcggt ttgtcatcag gtttgttttc tacctgatat ttccacttaa cttcccctgt
    14281 cttactatcg atggtgtacg gcagcgcacc atctacggca ggataacgtt catagaccca
    14341 aatgcccgtt gcgcgctgct gacgaacgcg gttcggatac ccttgcggat cgttatttac
    14401 taccgacttg ttcagtgcca tcgacagacc tgcggcatca agcagacgta caaagccaga
    14461 cgcgctctct tccttaagat tgctcatcac gttttccatg atcagcaccg atccaccttt
    14521 gttcagatag gcgatcagat cggtcacatc ctgctgagtc agcttcggtt tgctggtatc
    14581 tgctcgcagc gggattgcat aaggatcgtt accaacctga gtcacatact caaagccgtt
    14641 gaggatcagc agcggcattt cctgcgggtc gagatcgcca tagctactta aatgctcaac
    14701 agagataccc gcaaaatccg gatgaaagcc gaactcagcg ctgtttcctg taacctgacc
    14761 atgacgtttg aaatagacag tatccaggtt ggtgcctacg gtcatgctgg ctttcgcgtc
    14821 cggcgtccat ttatcgtcgg acagatagcg caacacgttc tgcataaagt gcttcatatc
    14881 attagaatca ctgttacgcg tacactgacc gtttttatca acaccgcctt cccagctgta
    14941 accgttcgga caacgcaaaa tgctgttgta gtgcgggtta ccgataacca tcagtttgcc
    15001 ctcaccgact tgccccagcg aaataaacgg caggttaaag gtggcggtat cgcgcgtaac
    15061 gttttccggc tcaacgagag aaggcgcttc cgtaatgtac gccagctcgt ttttatccca
    15121 ggcgcgtttt tcgccaaacg ccagccagta gtttttatca ttacgcgcca tcagaatcgg
    15181 gaaggccgcg ttggagatat tcaccactgc ctgaccgcgc gcattaccgg tactgccata
    15241 gaagttggta gagtcatgga agacgtggaa tttcgtcact gatttgtaat ctttatccac
    15301 accccacagc ttgttgataa cgccctgaat ctgaccttcg ttaacattgc gtgctgtcaa
    15361 cgagaaccaa cgctgagagt tgcagccccc aagggagtca caaatcgccg tatcaatctc
    15421 tttagcctgc ccggattcaa actgctcaag aaattcgttt gtccgctcaa aggtttgatc
    15481 gccttcgctc aacgcctcgc cattggataa cgagagattg ataatctcgt tgattacgtt
    15541 gggatactcg gcaaagacct tgcgcactac gtccggcact tcacgctcat cattttttcc
    15601 ggcctgggag taacgatgaa taagctgatc aatattcgcg ccgcgaactt cgtcacccag
    15661 ttcagtcaac gcaatggtcg acttattgcc gcgcactgag cccagttcaa aggtgtcgat
    15721 accaaaggag atggtttcgc cccagctgaa gttgaatttg ccgttttcgc cagttacgcc
    15781 acggcctgag ctggtgtagt aatttacgcc aaccacacca ttgcccatgc tatctaccag
    15841 tcggccttcg gagagaatga tttcagtggg ttgatactga taaaactgtt ccgcgttagc
    15901 cgacacgaag gaagcgttca gatccggttt tgttcccggc gtggtgacgg gcacaaccgg
    15961 tgaagtatga gtggatggcg ctttatcggt cgcggcattg ttttccacct cttcattgac
    16021 cagctttttg aactcttccg gtgccagatc gatttgctta tacagcgagt cgaaacgttt
    16081 actctcgatc actgaggaga acgtcaggca aacctgttct gtattcgccg gacagctgtt
    16141 actggacgtt accagcgaaa ccgcattgct tttcttgtca tcggaagctg ccagttcttg
    16201 cgcgtcctca agactaaacg acactttttc aaccgcacgc aggctacgcg cagcttctga
    16261 ctgggtgtcg aaggtagcaa ttgtcgtgtt acccgccacg caagtaacgt cctcaccagg
    16321 tgtaaatgta aagccatcgc tggattcacc attacaagta gcaccagtta cccgctggct
    16381 tccgcccagg gtcagataac ccgttttcgt aggaacaggt tctggctccg gttctggctc
    16441 aggatcaggt gtcggttccg gcgtaggttc tgggtccggc gttggctcag gcgtcggctc
    16501 cgggtttggt gttggatcag gtttcacttc cggcaaagac cctgttccag aatctacagg
    16561 cggcgtatcg gaggaagatc cggagccacc gccatcacaa ccggctaaca gggttgcact
    16621 caaaatagcc gctaaaagcg atttcttata tttaaatttc ttattcatta ataacgcaag
    16681 tgacaaaaca aagttaa
//
LOCUS       DAZFRG010000104        16321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFRG010000104 DAZFRG010000000
VERSION     DAZFRG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.104"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..1617
                     /gene="dgcJ"
                     /locus_tag="ACHKUC_002638"
     CDS             127..1617
                     /gene="dgcJ"
                     /locus_tag="ACHKUC_002638"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO6618878.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1660..2163
                     /gene="yeaK"
                     /locus_tag="ACHKUC_002639"
     CDS             1660..2163
                     /gene="yeaK"
                     /locus_tag="ACHKUC_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO6618879.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(2164..2268)
                     /gene="yoaI"
                     /locus_tag="ACHKUC_002640"
     CDS             complement(2164..2268)
                     /gene="yoaI"
                     /locus_tag="ACHKUC_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO6618880.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2438..2884
                     /gene="yeaL"
                     /locus_tag="ACHKUC_002641"
     CDS             2438..2884
                     /gene="yeaL"
                     /locus_tag="ACHKUC_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO6618881.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2841..3662)
                     /gene="nimR"
                     /locus_tag="ACHKUC_002642"
     CDS             complement(2841..3662)
                     /gene="nimR"
                     /locus_tag="ACHKUC_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO6618882.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3759..4940
                     /gene="nimT"
                     /locus_tag="ACHKUC_002643"
     CDS             3759..4940
                     /gene="nimT"
                     /locus_tag="ACHKUC_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO6618883.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAIIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLLGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4995..5342
                     /gene="yeaO"
                     /locus_tag="ACHKUC_002644"
     CDS             4995..5342
                     /gene="yeaO"
                     /locus_tag="ACHKUC_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO6618884.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5364..5618)
                     /gene="yoaF"
                     /locus_tag="ACHKUC_002645"
     CDS             complement(5364..5618)
                     /gene="yoaF"
                     /locus_tag="ACHKUC_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO6618885.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5801..6826
                     /gene="dgcP"
                     /locus_tag="ACHKUC_002646"
     CDS             5801..6826
                     /gene="dgcP"
                     /locus_tag="ACHKUC_002646"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO6618886.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            6859..6957
                     /gene="yoaK"
                     /locus_tag="ACHKUC_002647"
     CDS             6859..6957
                     /gene="yoaK"
                     /locus_tag="ACHKUC_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO6618887.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6960..7034
                     /gene="yoaJ"
                     /locus_tag="ACHKUC_002648"
     CDS             6960..7034
                     /gene="yoaJ"
                     /locus_tag="ACHKUC_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO6618888.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(7093..7341)
                     /gene="yeaQ"
                     /locus_tag="ACHKUC_002649"
     CDS             complement(7093..7341)
                     /gene="yeaQ"
                     /locus_tag="ACHKUC_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6618889.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7489..7671)
                     /gene="yoaG"
                     /locus_tag="ACHKUC_002650"
     CDS             complement(7489..7671)
                     /gene="yoaG"
                     /locus_tag="ACHKUC_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO6618890.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7675..8034)
                     /gene="yeaR"
                     /locus_tag="ACHKUC_002651"
     CDS             complement(7675..8034)
                     /gene="yeaR"
                     /locus_tag="ACHKUC_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO6618891.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8207..8845)
                     /gene="leuE"
                     /locus_tag="ACHKUC_002652"
     CDS             complement(8207..8845)
                     /gene="leuE"
                     /locus_tag="ACHKUC_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO6618892.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8972..9139)
                     /locus_tag="ACHKUC_002653"
                     /pseudo
     CDS             complement(8972..9139)
                     /locus_tag="ACHKUC_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            9140..9295
                     /locus_tag="ACHKUC_002654"
                     /pseudo
     CDS             9140..9295
                     /locus_tag="ACHKUC_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
     gene            complement(9388..10503)
                     /gene="rnd"
                     /locus_tag="ACHKUC_002655"
     CDS             complement(9388..10503)
                     /gene="rnd"
                     /locus_tag="ACHKUC_002655"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO6618893.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10585..12279)
                     /gene="fadD"
                     /locus_tag="ACHKUC_002656"
     CDS             complement(10585..12279)
                     /gene="fadD"
                     /locus_tag="ACHKUC_002656"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO6618894.1"
                     /translation="MKKVWLNRYPTDVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGF
                     LRIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKD
                     PSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(12484..13065)
                     /gene="yeaY"
                     /locus_tag="ACHKUC_002657"
     CDS             complement(12484..13065)
                     /gene="yeaY"
                     /locus_tag="ACHKUC_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO6618895.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(13105..13800)
                     /gene="tsaB"
                     /locus_tag="ACHKUC_002658"
     CDS             complement(13105..13800)
                     /gene="tsaB"
                     /locus_tag="ACHKUC_002658"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO6618896.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13858..15768)
                     /gene="yoaA"
                     /locus_tag="ACHKUC_002659"
     CDS             complement(13858..15768)
                     /gene="yoaA"
                     /locus_tag="ACHKUC_002659"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO6618897.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIKANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15900..16244
                     /gene="yoaB"
                     /locus_tag="ACHKUC_002660"
     CDS             15900..16244
                     /gene="yoaB"
                     /locus_tag="ACHKUC_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6618898.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4013 a         4209 c         3966 g         4133 t
ORIGIN      
        1 actattaccc ctaaatttcc cctccccagc ggcgcagaga tgagtataat tagcgcccct
       61 gtgccaggcc gcaatcgaac tttatctggt tttctcgttt cactaaccga aggagtgcca
      121 tttatcatga aattgcacca tagaatgctc cggcatttta tcgccgcaag tgtcattgtg
      181 ctgacatctt ccttccttat ttttgaactt gtcgccagcg acagagcaat gagtgcctat
      241 ctgcgctata tcgtgcagag agcagactcc tcctttcttt atgataagta tcagaatcag
      301 agtattgccg cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt gccgccagaa
      361 cagcgtcgcg ccatctgcga ggcttttgag tctgccaata atacccatgg cttaaacctg
      421 actgcccata aatacccggg cttacgcggc acattacaaa ccgcatccac tgactgcgac
      481 acaattgtgg aagctgcagc actattaccc gcttttgatc aggcagtgga aggcaaccgc
      541 caccaggatg attacggttc aggtcttggg atggccgaag agaaatttca ctattatctc
      601 gatctcaatg accgctatgt ctatttttat gagccggtta atgttgaata ctttgcgatg
      661 aataactggt ccttcctgca gtcaggaagt attggtatcg atcgcaaaga tattgaaaaa
      721 gtatttaccg ggcgtaccgt attatcgagc atttaccagg atcagcgtac taaacagaac
      781 gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc taaaagggat tgtgctgctg
      841 gatattaaca aaaacaatct gcggaatatc ttttacactc atgaccgccc tctcctctgg
      901 cgttttctca atgtcacgct aaccgataca gattcggggc gcgacattat catcaaccag
      961 agcgaagata atctgttcca gtatgtcagt tacgtccatg acttaccggg cggcattcgt
     1021 gtctcgttat ccattgatat tctttacttt atcacgtctt cgtggaaaag cgttctgttc
     1081 tggattttga cggcgttaat tttgctgaat atggtgcgga tgcacttccg cttataccaa
     1141 aatgtgtcgc gagaaaatat tagtgatgcg atgactggac tctataatcg caaaatttta
     1201 acccctgaac tggagcagcg gttgcagaaa ctggtgcaat ccggttcttc ggtgatgttt
     1261 attgctattg acatggacaa gttaaagcaa ataaatgaca ccctcggtca tcaggagggg
     1321 gatttagcga ttacgttatt agctcaggcg attaaacagt cgattcgtaa aagtgattat
     1381 gccatccgac tcggtggcga tgaattctgc atcattcttg tcgattcgac gccgcaaatt
     1441 gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc gcagaaagag
     1501 atcggcttct cttccggtat ttacgcgatg aaagaaaacg atacgttaca tgatgcgtat
     1561 aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa
     1621 tcttctgtgg ttgtttgctt gtaatctcag gagcatgaaa tgactgaaat ggctaaagga
     1681 agcgtgacgc atcagcggtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc
     1741 gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg caccgcttta
     1801 ggccaggggg ctaaggcact ggtatgcaaa gtcaaaggca atggcgtaaa tcaacatgtt
     1861 ctggcgatcc tcgctgccga tcaacaggcc gatctgagcc aactcgccag ccatataggc
     1921 ggattacgcg cttcgttggc cagcccggcg gaagtcgatg aactcacagg ctgtgtcttc
     1981 ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga ccccctactc
     2041 ttcgaacgat ttgatgaaat cgccttcaat gcaggcatgc tggataaatc cgttattttg
     2101 aaaaccgcgg attatttgcg tattgcgcaa ccagaactgg tcaatttccg ccgcactgcg
     2161 taactagcct gtccgttcaa taagcagaac ggacaaaacc agcacaactg caatggcaaa
     2221 aaacgatgac gtgataatca gtgtctcgac gaacatttga tcgttcatag tatgcccccg
     2281 ggagatagct gttttcacgt tattgttaac gtacacaaat ggcagtttga tgacagttcg
     2341 tctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcgtaaa
     2401 gtaagcaggc tcattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat
     2461 cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat
     2521 tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa
     2581 acaggggcta agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag
     2641 cgggacgcta ccgccctcaa ccctgattca ctcgtttctg aactggaaat cgctggtggc
     2701 aattgcagtc ggcgttattg tctcctggct gggggggcgc ggcgtgacgt taatgggcag
     2761 ccagccgcaa ctggtcgccg ggctgctggt gggcactgtt ttaggcgtgg cgctgtttcg
     2821 cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca
     2881 gtagttaatc ccgcaatata tcggcctggc gtttgcccca ggcctttctt aaacatagta
     2941 ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct
     3001 ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc
     3061 aaccccgtct ctttcactat cagccgggca agattgcgtt cactcatcgc aaaaaagcca
     3121 gcccattgcc ccaatgagcc ccactcgacg ggctctttcg ccatcatctc caccatcgtg
     3181 cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt
     3241 tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc
     3301 ctgtttgcca gggttaaaat taattcccgg cataagggcg atattttcaa tgtgcaacat
     3361 gttgtcggca ttgtcactgc gctgggctcg ataaacagaa aacagagttc agcgttagct
     3421 gtcacttgat tactatgttt caccccgccc ggtatccaaa ccgcatattg tggcggcacc
     3481 atccacaaag cattttccac cgtacaggta attgcaccat gtagtgccag tatcaactgc
     3541 cctttgcggt gctgatgtac aggactggtt aactcatccg ttccggcgtg aatacaaaat
     3601 gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat tgagattcaa cctgtgcatc
     3661 attttgtccg aatttagcga tattttgtca ttttagcttg attcaacata acaataaaaa
     3721 cggtaaggta cagcctcgtt tgtaacaatg agaagcatat gacctgttca acttcattaa
     3781 gcggcaaaaa caggattgtc cttatcgctg gcattctgat gattgccaca acattacgcg
     3841 tcacctttac cggcgcagca ccgttactgg atacgattcg ttccgcttac tcgctaacga
     3901 cggcgcaaac cggcttattg accaccctgc cattattagc ctttgcgcta atctcaccgc
     3961 tggccgcccc ggtggcgcga cgttttggta tggaacgtag cctgtttgcc gcgttacttt
     4021 tgatctgtgc aggtatcgca attcgctctc tcccttcgcc ttacttatta tttggcggta
     4081 cagcgatcat tggcggtggg attgcattag gcaatgtctt actgccagga ttaattaaac
     4141 gcgatttccc tcattccgtc gccagactta ccggcgcata ttccctgaca atgggagctg
     4201 cggcggcact gggatcggcg atggtagtgc cgctggcttt gaacggtttt ggctggcaag
     4261 gcgcgttgct catgctgatg tgttttcctc tgctggctct ttttttatgg ctgccacagt
     4321 ggcgaagtca acaacatgca aatttgagta cctcacgcgc cttacatact cggggtatct
     4381 ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg gatcaactca ctggtctatt
     4441 acgtgattat tggctggctt ccggcgattc tcatcagtca cggctatagc gaagcacagg
     4501 cgggttcact gcatggtttg ctgcaactag ccacagcagc tcccggtttg ctgatcccac
     4561 ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc ttgatgtgcg
     4621 cagtgggcgc ggttgggctc tgctttatgc cagcacacgc gatcacctgg actctgcttt
     4681 tcggttttgg ttccggcgca acaatgatac tggggttgac gttcattggt ctgcgggcaa
     4741 gttctgcgca tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg
     4801 cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc taacggtgac tggtccgtac
     4861 cactgctggg tgttgcaata ctttcactac tgatggcgat tttcggactt tgcgccggga
     4921 gagacaaaga aattcgctaa tatccggtgc tatagtgacg tgacaaatca tgcgtgaaag
     4981 ggagaacaaa tacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg
     5041 atggttatcg cgtactggtc gaccgcctct ggccacgcgg tatcaaaaaa accgatttag
     5101 cccttgatga atgggataaa gaaatcacgc cgtcaacgga gctgcgcaaa gcctttcacg
     5161 gcgaagtcgt cgattttgca acctttcgcg agcaatatct tgcagaactg gcgcaacacg
     5221 agcaagaagg aaagcggctg gcagacatcg ccaaaaaaca gccgctgacc ctgctctact
     5281 cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc agactggcta cgtagcttgt
     5341 gattttagta cagcatccgg cggttatttt tcaccagccg gatggtcccg tcgccacaat
     5401 gcccattcat caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct
     5461 gtctgcacag gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc
     5521 ataccaatct gcggcggttt aggtgcttct ggctgagaag gggttgaaca accagccagg
     5581 accagcaagc aaggcagaac aaaactgata attttcattt attgatctca catatttatc
     5641 caggcttaga gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc
     5701 aaaacatatt acgccttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt
     5761 aaataatcag catttacccg ccgtatcctg gagttgtttc gtgtcagatc agattatcgc
     5821 ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa agtctggttc gacagcttct
     5881 ggagatgctg gaaatggtca ctgatatgga gtcaacctac ctgaccaaag tggatgtcga
     5941 agcgcgcctg cagcatataa tgtttgcccg taatagccag aaaatgcaca tcccggagaa
     6001 ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc attgatgaaa actgtttttt
     6061 cagcgatgaa gtccccgacc gttggggcga ctgtgttgcg gcacgcaatc ttggcatcac
     6121 cacatttctg agcacgccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc
     6181 cgccagcagt gagaagcgcc agtggagtga acgcgcggaa caggtcttac agttgttcgc
     6241 cggactgatt gcacaatata ttcaaaaaga ggcactggtt gaacagctgc gcgaagctaa
     6301 tgctgcgctg attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc
     6361 gatttttgaa aatctgacga cgctgttttc tctcgcccgg catcttaacc ataagataat
     6421 gatcgcgttt atcgatctgg ataacttcaa attaatcaat gatcgttttg gtcataatag
     6481 tggcgatctg tttctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga
     6541 agttattggt cgtctcggcg gtgatgagtt tttggttgtt tcactgaaca acgagaatgc
     6601 ggatatttcg tcgctgcgag aacgtattca acaacaaata cgcggagaat atcacttagg
     6661 tgatgttgat ttgtattatc ctggtgccag tcttggcata gtagaagtcg atcctgaaac
     6721 gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg atgtatcagg agaaaaaaca
     6781 taaacagaaa acaccttttg tcacgcattc agcgctacat tcctgaggcg tattcacatc
     6841 cttttgattg gtgataacat gcgaatcggt attatttttc cggttgtaat ctttattaca
     6901 gcggtcgtat ttttagcatg gttttttatt ggcggctatg ctgccccggg agcataaaga
     6961 tgaaaaaaac aacgattatt atgatgggtg tggcgattat tgtcgtactc ggcactgagc
     7021 tgggatggtg gtaacgtcac atctaaaaaa tagcaaaggc tgcctgtgtg cagcctttgt
     7081 gcaatttacg cgttaacttt taatcttcct gtagataaat agcacaacaa ttgcaccaat
     7141 aacggcaacc acgaagctgc caaaattgaa gccatcgact ttaccaaagc caaacagcgt
     7201 gctgatccat ccgccaacta cggcaccgac tatccccagt aggatagtca taaagaatcc
     7261 accaccatct ttacctggca tgatccactt cgccagaata ccggcaataa gcccaaaaat
     7321 aatccatgac agaatgccca ttgtttcctc acttatctgt tttgcattag cgggttagtc
     7381 gctgataaaa agcatagcac aacatcggga gggtaagatt tgtgacgagc atcacggagg
     7441 aattttttgc gatggcgcag aaattgcgcc atcaacgatc agtgataatt accaaccaca
     7501 aacatcatgt tcgttttccg tgtcataaga acgcacggta ttcaccagat cttttatcac
     7561 ttcagccgcc acttctggca ccagcaaagt catcggcgtc tctgtttcat aatcgacaga
     7621 aacgccattg ctgttattgg tgacggtcac ggtatacgtt gctttgccca tgattcattt
     7681 cccgttatga atgactttcc gttgttgcgc gccttccatc aggacttcag gagcaacgaa
     7741 gaagtcaatg ttgaaataag tatcgtcagt catggcttca atgttgtgcc acttttctgg
     7801 agggaacacc gcaaactgcc ccgcttcgat aaggatcacc tgatcaggct ctgcactgtg
     7861 ttcatcagcg tagccgagat atttgaccgc cccatgcata acggaaaggc gtgggtaaac
     7921 ccccgggcgc gttcctttat caagatgacg ttcgaatatt ccggcaggcg cagtttgttt
     7981 attccagaaa ggcgttgagc gcgtatgaat ataattctgt gggatttgca gcatcctttt
     8041 ccctccttcg atgaatgcgc tgaaaacggt ttattccagc cgtttcaggg tacgcctgat
     8101 aatttgcatt ttaaatacca tttattggtt actttttagc accgtatcag agaagaatca
     8161 gggaggatta caggtgggaa gcccgcggca acgcgggctg aaagcatcag gattgcagcg
     8221 tcgccagtcg ggcggcgaaa cccacgaaca tcaaaccaat cagggagttg cccactttag
     8281 ccagtttctt tttggtacgt atgtactgcg tgacaaaagc cccagaaata atcagaaagc
     8341 tcaaatagca gaaactcacc agttccagcg tcgtcgccag aataaagaat gaaattcccg
     8401 tatgtggggc attaacatcg ataaactgta cgaaaaacga cacatagaac aaaatggctt
     8461 tcggattagt caggctcaaa attaacgcgc gtttaaaaat ggcaccgtat tggggctcat
     8521 cggatttggc ctcgctattt ttacccttca gggttgcgta gagaatttta ctccccagat
     8581 agagcaaata aaacgcacca agataacgta cgatgttgaa taatatcggg gtggtcttaa
     8641 ttaatgtcgc cactccagcc catgccagaa acatcaatac cgcatcgcca ataaatacac
     8701 cacaggccgc aagataaccg cctttcatac cgctactgac gctatttttg agtacaaaca
     8761 gggtatttgg ccctggcacc aacacaataa aaatggcccc aaccagatag gtccagtaat
     8821 tcagaacccc gtattcagcg aacacattaa cctctttaat tatctttcga tcatgcgcga
     8881 ttaaaggtga atatgctaac caatctgtag cggcttagaa aggagaaaat caggttttaa
     8941 ctcgatatca acccgataat tgaatcatta atcaggcatg ctccagtgaa aaattcgggt
     9001 agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat ccgcactttc gctgacgtcg
     9061 ccagccttga aacataaacg gcccagacat tcgctggctg gtaatattcc ggtagcactt
     9121 gtactaaatg accactggcg ggaaacagcc tggcgacatc gatttttacg tggtccggaa
     9181 aaacaccgaa ggcgaatatt cctcgccagg cggtagagta aatgaaggaa cagagaatga
     9241 agtcgtcatt caggaatcgg tatttactcg tcgtggtgtc gagcgcattt tgcgttaagc
     9301 tcaaataagt tcatttgaac cacctgctgt tggatttgta gtttgccgaa gccggactga
     9361 gcgcgcccag tccggcttcg gaagatttta ctgtggatat tcctgcaata aattgtgtaa
     9421 tgcttccgcc atcaactcac cacgccagcc ggaaatcagc tccggcaaat tgttctgcgg
     9481 tttcagtttc cagtgccagt tcagcagttg gttgatttgc cgacgcgacg ccagcaattc
     9541 ggcgctgatc ttgtgcgttt cgctgacgtc agtaatcagc gacttaatcg ctttaaacgc
     9601 tttacgataa cccggcatgt ccatcaggtt aagcatcggc tgcggtaagg cctcttctgg
     9661 caatgcctgc gctttttcca ccagcgccag caacgtttta ccgtggaagc ggatttcgct
     9721 accggataag cccaggctgt ccagttcgcc cagacttccc ggcatataac gcgctaccga
     9781 ccacaaatgc tcttcacgca cgacaaagtt caccgccaga tcgcgctctc gcgccttgcg
     9841 caatcgccag tcggctaaca gttgcagaca ggccagttgg cgtgtgcgta actgccaggc
     9901 attggtgata tcacgccagg cgtcttccgg cgcaacgact tcctgacgac gcatttgcat
     9961 caggcggcat tcatccagcg ccgcaggtag ccagccggag gcctccgttt ctaccataag
    10021 cttggcggtg atcggtaaca gataccagac atccgccgct gcatattcac actgacgttc
    10081 ggtcagcggt ctggccagcc agtcggtgcg cgattcactc ttgtccagcg taacgccgga
    10141 atactcttcc accatggaag cgaaacccca tgacatcggg cgtccgcaga aggcagcaag
    10201 gatttgcgtg tcaatcaggg gttgtggtaa ttcgccaaag acattgagga acacttccag
    10261 atcttcactg cctgcatgga gaaattttgt gatggacgga tcgcgcagga tcgctttcag
    10321 tggtgaccag tcggagatcc cgagaggatc aattagcgcc agatgctcac catcaaaaag
    10381 ttgaattaga cccagttgcg ggtaataggt gcgcgtacga acaaattcag tatccagggc
    10441 tatcgccgga aaggcacgga cggcttcaca caaagaagcc agcgcatcgt ccgtggtaat
    10501 catttggtaa ttcaaattgt attctcttta gtgggcgtca aaaaaaacgc cggattaacc
    10561 ggcgtctgac gactgactta acgctcaggc tttattgtcc actttgccgc gcgcttcgtc
    10621 acgtaattct cgtcgcaaaa tttttccgac gttagatttc ggtaactcat cacgaaactc
    10681 caccagcttc ggtactttgt agcccgtgag ctgacggcgg caaaaggtca ccagtgactc
    10741 ttcggtaagc gatggatctt ttttcactac gaagattttc accgcttcac cactggagcc
    10801 ggaaggtacg ccaacagccg cgacttcctg tacgccagga tgctgcatga cgacatcttc
    10861 aatctcgttg ggataaacgt taaaaccgga aaccagaatc atgtcttttt tacgatcgac
    10921 aatgcgcagg aatccttctt catccatcac cgcgatgtcg ccggtgtgta accagccatt
    10981 tttgatgatt tcatcggtag catccggacg ctgccagtaa cccagcatca cctgcggtcc
    11041 tttgacacaa agctcacctg gttgaccagg tggtacttca ttatcatcat tatcatcatc
    11101 atccaccagt ttggcttccg tcgacggcac cggcaaaccg atgctaccac tatgataatc
    11161 aatatcatac gggttaacgc tgaccagcgg cgcgcactcg gtcaggccat aaccttccag
    11221 cagatactgt ccagtcagtt taacccaacg ttctgccacc acttgctgaa caggcatccc
    11281 tccgcctgcg gaaagatgca gactggagaa atccagctgc tggaactctt tattgttcag
    11341 caacgcattg aacaaggtgt taacaccagt gatagcggta aacggatatt tcgctaactc
    11401 ttttaccaac cctggaatat cgcgcgggtt agtgataagc aggttctgcc cccccagttc
    11461 gataaacagc aggcagttaa tggtcagtgc aaaaatgtga tacagcggca gcgccgtcac
    11521 caccagctct ttgcccggat gcaacagcgg accataggtc gcgttaacct gttccaggtt
    11581 cgccagcata ttgcggtgag tcagcatcgc gcctttcgcc acaccagtgg tgccgccggt
    11641 gtattgcagg aaagctaaat cttccggcac cagttctggt ttgacgtact gcatccggta
    11701 gccgttgtgc agggcgctac gaaatgaaat ggcatctggc agatggtatt tcggcacaag
    11761 acgcttgatg tatttaacaa cgaaattgac taccgtgcct tttgccgtag atagctgatc
    11821 gcccatacgg gtcagaataa cgtgctgaac ggcggtttta tcaaccactt tttccagagt
    11881 gtgggcaaag ttagacacga taacaatcgc cgatgcgccg ctatcgttaa gctgatgctc
    11941 aagctcacgc ggggtataca acgggttaac gtttacgacg atcatcccgg cacgcaaaat
    12001 gccaaacagc gccaccggat attgcaataa gttaggcatc atcaacgcaa cgcgatcgcc
    12061 tttcttcagc cccaaccctt gttgcaaata agcggcaaac gcgcgactgc gttcttccag
    12121 tttgcggaag gtcatcacct cccccatatt cacaaacgcg ggttgatcgg catagcgcgc
    12181 gaccgactgc tcaaacatat ctaccagaga ttgataacgg tcagggttga tctccgtcgg
    12241 aacgtccgtg ggataacggt taagccaaac cttcttcaat tcttcacctc taaaatgcgt
    12301 gttcgtcgtc atcgcaaccc caagtgatag acatgctgtt aacataatat taactcatct
    12361 taccagcttg ataattaccc aaggaaaagg ttgcgaagcg cgtcactatt tatttttatc
    12421 tttaccgtaa gaatgcagaa acagcggacc ggccgctgtt tctttttctt taaaaacaag
    12481 caattattca gttacaactg tttgtactct cgcggggccg ggattatacc agccccatcc
    12541 accgtagcca tagggccagc cacgaccgcc ataaaaccat ggatcaatcg gctgaggcgg
    12601 cataatcacc tgctgggtta aatgccaacg tttgtaacct gttgcctgca tcaccataaa
    12661 tttatagggc gtgttgccga tttttccgtc aaccgtaccg gtgattggcc cgacaactgt
    12721 caccagctgc ccacggaaat cgaccggatc aagaaaacca ttcacatcgg cataaatgcg
    12781 accgcgagaa ggttctccca gcgtcggtct ggcaccgctg tccagcggta cggtagcaat
    12841 ttccaggcgg gttttccctt gctggttttg taccgcaacc actttgccgc caaagcgtgc
    12901 ctcctgacca acgtacagct gcggcgcact catcacccga actaaatctt gttgcggcgt
    12961 gggactgctg cctttaatgg cgtccggcac agtaacacaa ccgctcagca ttagcgcaaa
    13021 cgtacctgcc agtatgcctt tgataacatt cttttgaacc gccatgatgc gactcctttt
    13081 tttctcaggg catactctta agattcattc tttgcccgga agtttcttcc atgcgacgtt
    13141 gttacgtaaa taaactggtt cagcatgttc caccgccacc gttttaccct cggcaaacat
    13201 ctggcacgca atcggcagca tatcttctgc agcgggcagt aacacttcgc catcgcgcaa
    13261 aaccagcccg ctctctttac cgagatccgg ccaggcttgc cagcccgtcc ctaccgtcac
    13321 ccattcgccg gaaagctgtt gcattcgttc atgaaccagt tcgggtttga gtacggcttc
    13381 ggtttcttca ccatgccaga taccgttttc atcacgctga tattcggccc agtaaacttc
    13441 gcccattcgc gcgtcaatag ctgccagcac gcgggttgcg ccgtttttgc gccacgcccc
    13501 ttgcgccatc gtcattagcg tggagacgcc aatcatcggc agctctgcgc caagtgccag
    13561 cccttgcgca atgccaatac caatgcgcac gccggtaaag ctaccggggc cgcgcccgta
    13621 agccagagcg tttatgtcag tcagagaagt tccgctggtg gtcaggatat cctgcaccat
    13681 cggtaagatt cgttgagtat gttcacgagg gcaaagctca aaatgagcgt tgacagtacc
    13741 gtcgttccac agggcgacag agcaggcctc tgtcgcggta tcgatagcca gaattcgcat
    13801 gggtcttcgt gcttagatca ataaaaaggc gcgcatcata ccatactccg taacaaatta
    13861 cctggaggat ggtatcgcaa ggaaacgaac cgcacgggca atgtcgcggg tgcgcggcgc
    13921 tggcggcaga ctggcgagaa acgtcgcgcc gtaagggcgc atcaccagcc gattgtcgca
    13981 aatcactaac acgccgcgat cgtcggcgtc gcgaatcaat cgccctaccc cctgcttgag
    14041 agtaatgacc gcatccggca gttgtacttc atcgaacgga tcaccaccgc gcaaacggca
    14101 atcttccatg cgtgctttta atagtggatc atccggcgag gtaaacggca atttgtcgat
    14161 aatcaccaat gacaatgtat cgccacgcac gtccacccct tcccagaaac tgctggttgc
    14221 cacaagaagc gcgttaccgg cgctgacaaa ttgctgcaac agttgccctt tgctggtttc
    14281 cccctgcaac aaaacgggaa gcgtcatggt agcgcggaac tgctcggcca gatcgcgcat
    14341 catggcgtgc gaggtacaaa gcataaaaca acgaccgttg ttagctttga tgatcggtcg
    14401 cagcattgcc gccagttgcc gtgcggaacc cggttgattg gtttgcggca ggttgcgcgg
    14461 cacacagagt aacgcctggc ggctgtaatc aaaggggctg ggtaacagca acgactctgc
    14521 ctgttcaatg ccaagccgcg aggtgaaatg atgcagatcg tcgtttaccg acagcgttgc
    14581 tgaggtaaag atccagctac cggttttttg cgccattaac tctttgaatt tatccgccac
    14641 gctgagcggc gtgagagcca gagtaaaatg gcgcgaagtg cattcgtacc agtagctgta
    14701 gcccggctga ttgatctctt ttagccgttt cagccgtgtg cgatataacg tggcgcgttc
    14761 aaatgccgca tccagcaagg cggaacgccc cagcgacagt ttcgccacgt cataacaaag
    14821 ttccagagtg tcatcgagca gtaaaaatgc ccgctgaatt tgcggattag ctaacagctc
    14881 acgcaggtta ccgcgataac ctggctcacc gagttgcagg cgaaaatcct gcgcactctg
    14941 ggcaagacgg tccgcgcact tttgtaactg ctgggtgtct tttaattcgg tgcggtaggc
    15001 gatggtgatg tcttttgcca ggtcgagcag ttgtcgactg gagagtgact gaccaaaata
    15061 ctggctggca atgtcgggca gctggtgggc ttcgtcgaag atcatgacgt ccgcttccgg
    15121 gatcaattcg ccaaaaccgc tctctttcac caccatatcc gccagaaaca gatgatgatt
    15181 caccaccacc acatcggcgt ccatcgcttt tttgcgtgct ttgaccacaa agcaatcttt
    15241 atacatcggg cagtcactgc cgaggcagtt atcgttggtg ctggtgacca gcggccacgc
    15301 ctgtgaatct tccgccacgc tgacgcaggt gctgatatcg ccatcgactg tttgattaga
    15361 ccaggagcgc agcaggatca catcgcttaa gatttgtacc ggcagatcgc cccccgccag
    15421 cgcctgctgt tcgagacgtt cgaggcagag gtagtttgag cgccctttca gcagcgccac
    15481 attgcccgtg tatttcaatg cctttgagac cgttggcaaa tcgcggctgt agagctgatc
    15541 ctgcaacgct tttgagccgg tcgagattat gactttcttt ttcgcccgca gcgcaggggc
    15601 caggtaagcg taggttttgc ccgtaccggt tcctgcttcc accaccagcg gctggccttt
    15661 ttctatcgcc tgggtgacgg ctaccgccat ctgtcgctgt ggttctcgcg gcttaaagcc
    15721 tggtatcgct ttcgctaact gaccgtctgg tgcaaaatcg tccgtcacac taccccctgt
    15781 tgatttgaac agggattatg tcaggatgag ggcgctttcg ccagttgaag tggtgacggc
    15841 gacctcacat tgtggcagtc tttgcagcac gaaaatggga gtataatgag gacaaaatga
    15901 tgactatcgt tcgtatcgat gccgaagccc gctggtctga tgtggtaatc cacaacaaca
    15961 cgctctacta cactggtgta ccggaaaacc tcgacgccga tgcctttgag caaaccgcca
    16021 acacgctggc gcagattgac gccgtgctgg aaaaacaggg cagcaataaa tcgagcattc
    16081 tggatgccac cattttcctg gccgataaaa acgacttcgc ggcgatgaat aaagcgtggg
    16141 atgcctgggt tgtcgcgggt catgcgccgg tgcgctgcac ggtacaagca ggtttgatga
    16201 acccgaagta taaagttgaa attaagattg tggctgcggt gtaagcttta tcgaagcaaa
    16261 ataagtcaga cgataattta tcgacaatac tggtcggttt tgcataaatc gaccagagat
    16321 t
//
LOCUS       DAZFRG010000105        16052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFRG010000105 DAZFRG010000000
VERSION     DAZFRG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.105"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1566
                     /locus_tag="ACHKUC_002661"
     CDS             <1..1566
                     /locus_tag="ACHKUC_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO6618899.1"
                     /translation="VPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMT
                     TRVFRRQFSEMTDLVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIER
                     ANKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVR
                     KLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIATVNDFDSSFGYRNLRIHK
                     SAICEPLQGENAWSYAEKLKLAISSIRNHMFSEFIFCDDAKLNEIEENIWIARNIRHA
                     MEIGELFLVYQPIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGY
                     QIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLC
                     VEITETVIERINEHFYLNIEQLRKQGVRVSIDDFGTGLSNLKRFYEINPDSIKVDSQF
                     TGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMP
                     FSAWDKSGKLVKE"
     gene            1574..2902
                     /locus_tag="ACHKUC_002662"
     CDS             1574..2902
                     /locus_tag="ACHKUC_002662"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6618900.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2949..4274)
                     /gene="rimO"
                     /locus_tag="ACHKUC_002663"
     CDS             complement(2949..4274)
                     /gene="rimO"
                     /locus_tag="ACHKUC_002663"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO6618901.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4487..4870
                     /gene="bssR"
                     /locus_tag="ACHKUC_002664"
     CDS             4487..4870
                     /gene="bssR"
                     /locus_tag="ACHKUC_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO6618902.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            4981..6096
                     /gene="yliI"
                     /locus_tag="ACHKUC_002665"
     CDS             4981..6096
                     /gene="yliI"
                     /locus_tag="ACHKUC_002665"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO6618903.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6093..6719)
                     /gene="gstB"
                     /locus_tag="ACHKUC_002666"
     CDS             complement(6093..6719)
                     /gene="gstB"
                     /locus_tag="ACHKUC_002666"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO6618904.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6965..8167
                     /gene="dacC"
                     /locus_tag="ACHKUC_002667"
     CDS             6965..8167
                     /gene="dacC"
                     /locus_tag="ACHKUC_002667"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6618905.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8214..8972)
                     /gene="deoR"
                     /locus_tag="ACHKUC_002668"
     CDS             complement(8214..8972)
                     /gene="deoR"
                     /locus_tag="ACHKUC_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO6618906.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLIEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9030..9626)
                     /gene="ybjG"
                     /locus_tag="ACHKUC_002669"
     CDS             complement(9030..9626)
                     /gene="ybjG"
                     /locus_tag="ACHKUC_002669"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6618907.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9911..11143
                     /gene="mdfA"
                     /locus_tag="ACHKUC_002670"
     CDS             9911..11143
                     /gene="mdfA"
                     /locus_tag="ACHKUC_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO6618908.1"
                     /translation="MQNKLASGVRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11184..11468)
                     /gene="ybjH"
                     /locus_tag="ACHKUC_002671"
     CDS             complement(11184..11468)
                     /gene="ybjH"
                     /locus_tag="ACHKUC_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO6618909.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11554..12369)
                     /gene="ybjI"
                     /locus_tag="ACHKUC_002672"
     CDS             complement(11554..12369)
                     /gene="ybjI"
                     /locus_tag="ACHKUC_002672"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO6618910.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            complement(12369..13577)
                     /locus_tag="ACHKUC_002673"
     CDS             complement(12369..13577)
                     /locus_tag="ACHKUC_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6618911.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13661..14197
                     /gene="rcdA"
                     /locus_tag="ACHKUC_002674"
     CDS             13661..14197
                     /gene="rcdA"
                     /locus_tag="ACHKUC_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO6618912.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            complement(14372..16051)
                     /locus_tag="ACHKUC_002675"
     CDS             complement(14372..16051)
                     /locus_tag="ACHKUC_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO6618913.1"
                     /translation="MNVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVLVV
                     SLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGSAL
                     VIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLSLG
                     YALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAYRV
                     GPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYPDA
                     HARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRVIR
                     SQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVIGL
                     MIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLMVF
                     MAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGAMM
                     GARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         4162 a         3854 c         4026 g         4010 t
ORIGIN      
        1 gtgccaaaca acgcattatt cgaaatctat ttttattatc ctggtggtaa tttattgaac
       61 gcatcagata aactttttta tctgccgttt gctttcatta ttatcgtatt gctggtggtt
      121 tatttaatga ccactcgagt gttccgtcgg caattttctg aaatgacaga tttggtcaat
      181 acgctggcgt ttttgcccga ctctacggat caaatcgagg ctctgaagat tcgtgaaggc
      241 gatgcgaaag agattatcag catcaaaaat tcgatcgcgg aaatgaaaga tgctgaaatt
      301 gaacgggcaa ataaattgct ctcgctgatc tcttacgatc aggaaagcgg ttttattaaa
      361 aatatggcga ttattgagtc caacaataat cagtatctgg ctgtggggat catcaaactg
      421 tgtggtctgg aagctgtgga agcggtgttt ggtgttgatg aacgcaataa aatcgtcagg
      481 aaattgtgtc agcgaattgc cgagaaatat gcgcaatgct gcgatatcgt gacatttaat
      541 gccgatctct atttactcct gtgccgggaa aatgtacaga cgtttacccg taagatagcg
      601 acggtaaacg attttgacag cagttttggc taccgcaatc tgcgcatcca taagtctgct
      661 atttgtgaac ctttgcaggg ggaaaacgcc tggagttacg cagaaaagct gaaactggcg
      721 atttccagta tccgcaacca tatgttctca gagtttattt tctgtgatga tgcgaaactc
      781 aacgaaatag aagagaatat ctggattgcg cgtaatattc gccatgcaat ggaaattggc
      841 gaactattcc tggtctatca accgattgtt gatattaaca cccgcaccat tttgggcgcg
      901 gaggcgttgt gccgttgggt gtctgcggag cgggggatta tttcaccgct gaagttcatt
      961 accattgctg aagatatcgg gtttatcaat gagctgggct atcaaattat taaaaccgcg
     1021 atgggtgaat tcagacattt tagtcagcgt gcgtcgctga aggatgattt cttactgcat
     1081 attaatgttt cgccctggca gttaaacgaa ccacactttc atgagcgttt tactaccatc
     1141 atgaaagaaa atggcctgaa ggcgaacagc ctttgtgttg agatcaccga aaccgtgatc
     1201 gagcgaatta atgaacattt ctatctcaat attgaacaac tgcgtaaaca aggggtacgg
     1261 gtatcgattg atgactttgg caccggtttg tcaaacctga aacgttttta tgaaattaat
     1321 ccagacagta taaaggtgga ttcgcaattt accggcgata ttttcggtac tgcaggaaaa
     1381 attgtgcgca ttattttcga tctggcacgc tataaccgga tcccggtgat tgcggaaggc
     1441 gtagagagcg aagacgttgc gcgtgaatta atcaaattag gatgtgttca ggctcagggg
     1501 tatctgtacc agaaacccat gccattctcc gcctgggata aaagtggaaa attagtaaaa
     1561 gagtagttta cgtatgtcca gaatcaataa attcgtactt acagtcagtc tgctgatttt
     1621 tatcatgatt tcagcagttg cctgcgggat ctacactcaa atggtaaagg aacgggtgta
     1681 tggcctgaaa cagtccgtta ttgatactgc ttttgcggtg gcaaatattg ctgaatatcg
     1741 gcgtagtgtg gcaattgatc tcatcaacac gctaaatcct acggaggaac agctgttggt
     1801 tggcttacgc acagcttacg ccgactcggt ttcaccctct tatttgtacg atgtcggtcc
     1861 ttatctgatt tccagtgacg aatgtattca ggtaaaggag ttcgagaaaa attattgtgc
     1921 agatattatg caggttgtta agtatcgaca tgtcaaaaat acagggttta tctcttttga
     1981 tggtaaaacc ttcgtctatt acctctatcc ggtaactcac aatcgtagtc tgatattttt
     2041 gcttggtctg gaacgttttt ctttactgtc aaaatcgctg gcgatggata gcgagaacct
     2101 gatgttctcc ctgttcaaga acggtaaatc ggtgaccggt gatgaatata acgctaaaaa
     2161 cgccatcttc accgtttcgg aagcaatgga gcacttctcc tatttgccga ccgggttgta
     2221 cgtatttgct tataaaaaag acgtttattt tcaggtatgt acattgatta ttttctttgc
     2281 cgcattggtg gcagtgatat cgggtgccag ttgcctctat ctggtacgca gagtgattaa
     2341 tcgtggtatt gtggagaaag aagccattat taataaccat tttgaacgcg tactggatgg
     2401 cgggcttttc ttttcggctg ccgatgtcaa aaaactctac agtatgtata actcggcatt
     2461 cctggacgat ctgaccaaag cgatgggcag aaaatccttt gacgaagatt taaaagcgct
     2521 gccggaaaaa ggcggttatt tgtgcctgtt tgacgtcgat aaattcaaaa acattaacga
     2581 caccttcggt catttgctgg gcgatgaagt gttgatgaaa gtggtgaaaa tccttaaatc
     2641 acagatcccg gtagataaag gtaaagttta ccgcttcgga ggtgacgaat ttgcggtgat
     2701 ttatactggc ggcacgctgg aagagttgct atcgattcta aaagaaatcg ttcatttcca
     2761 ggtgggaagc attaatttaa gcaccagtat cggtgtagcg cattcaaatg aatgtactac
     2821 cgttgaacgt ttgaaaatgc tggcggatga gcggctgtat aagagtaaga aaaacggcag
     2881 ggcgcagatt agctggcagt aatcattatt cgcaggccgg acaaatgatt ttgcccggcc
     2941 tgaattaatt aaacccggct accccacaaa tcgtactcat cggcgtgctc gactttcact
     3001 cgcaggatat cacccggctt aacgttggtt tcaccattga gataaaccgc gccgtcgatt
     3061 tccggtgcat ctgccatgct gcgaccaatc gcgccttctt cgtccacttc gtcgataatc
     3121 accagaattt cacggcccac tttctcttgc aggcgctcgg cggaaatttg ctgctgcaac
     3181 tgcatgaaac ggttccagcg ttcttctttc acttcttccg gaacctggtc aggcagggca
     3241 ttggcgtctg caccttcaac cgggctgtat ttaaagcagc caacgcgatc cagacgtgct
     3301 tctttcagga agtcgagtag catctggaaa tcttcttccg tctcgccagg gaagccaaca
     3361 ataaaggttg agcgtagggt cagttccggg cagattttgc gccactgttt gatgcgcgcc
     3421 agttggcgat ctacagaacc cggacgtttc atcagtttga gaatgcgcgg gctggcgtgc
     3481 tgcaacggaa tgtccagata cggcaggatt ttgccctctg ccatcagtgg gatgacgtcg
     3541 tccacatgcg gataagggta aacgtagtgc agacgtgtcc agatccccag tttcgataac
     3601 tgttcgcaca ggctgaccat gctggttttt accggctcgc cgttgtggaa gccagtacga
     3661 tgtttaacat cgacgccata cgcggaagta tcctgcgaga tcaccagaat ttctttaacg
     3721 cccgcatcaa ccagacgttt cgcttcactt aacacttcgc caatcggacg gctcaccaga
     3781 tcgccgcgca tagacggaat aatgcagaag gtgcagcggt gattacagcc ttcagaaatt
     3841 ttcagatagg cataatgacg cggcgtcagt ttcacacctt gttctggcac caggctcagg
     3901 aatgggttgt gtttcggttt tggcacgtag tgatgaacgt gctccagaac ctgctcatag
     3961 ctatgaggcc cggtgatttc cagcactttc gggtggactt cgcggatctg atcttctttt
     4021 gcccccagac aaccggtcac aattaccttg ccgttttcat tcaacgcttc accaatggct
     4081 tccagtgatt cttgtaccgc gctgtcaata aagccgcagg tgttgacgat caccatgtcc
     4141 gcatcatcat agctcggtac cacgtcataa ccttcagtgc gtagttcggt gagaatacgc
     4201 tctgaatcga caaggttttt cggacagcca agggaaacaa agccgatttt cggctgggga
     4261 gttactttgc tcatagctta aaaaatattc agttacagga aaggtcaggg cagggattct
     4321 acagagttct ggataaaatt tgtatcgcaa tctcattcgc tggcggagtt gaaggaaatg
     4381 taaattttgt taattcggcg tgaagaattg atcctggaca gcattttgct caaaaaacag
     4441 ccatactatt taattgcaac aaggctggaa agaggaggat cgaagtatgt tcgttgacag
     4501 acagcgaatc gatctgctga accggttgat cgacgcacgc gttgacctcg ccgcatacgt
     4561 gcaactgagg aaggcaaaag gatacatgtc cgtcagcgaa agcaatcatc tacgagataa
     4621 cttttttaaa ctgaatcgcg aactgcacga taaatcgctg cggttgaatc ttcatctgga
     4681 tcaggaagag tggagtgctc ttcatcatgc tgaagaagca ttagcgacag ccgcagtatg
     4741 tttgatgagt gggcaccatg attgcccgac tgttattacc gtcaacgccg ataagcttga
     4801 aaattgtctg atgagcttaa cgctgagtat ccagagcctg cagaagcacg ccatgcttga
     4861 gaaggcctga aaactaaggg ggagaaagcg tctccccctt catgtttaag tttttgtaaa
     4921 aatgaatttg ttatctcctc cactgactac gctttaagcc agagtcaatc cggaggcgtt
     4981 atgcatcgac aatccttttt ccttgtgccc cttatttgtc tttcttccgc tctctgggca
     5041 gctcctgcaa cggtaaatgt cgaagtactg caagacaaac tcgaccatcc ctgggcgctg
     5101 gcctttttac ccgataatca cggtatgtta atcactctgc gcggcggcga gttgcgtcac
     5161 tggcaagcag gaaaaggatt atctgcgccg ctttccggag tgccggacgt ttgggcgcac
     5221 gggcagggcg gtctgctgga cgtggtttta gcgcccgatt ttgctcagtc gcgccgcatc
     5281 tggttaagtt attccgaagt tggcgatgat ggcaaagccg gaactgctgt gggttatggc
     5341 cgcttaagtg atgatctctc aaaagtgacc gacttccgca ccgtctttcg tcagatgcca
     5401 aaactgtcta ccggcaacca ttttggcggg cggatggtat tcgacggtaa aggttatctt
     5461 tttattgctc tgggcgaaaa taatcagcgc ccgacggcgc aggatctgga taaattacag
     5521 ggcaaactgg tgcgtctgac cgacctggga gaaatcccgg atgataatcc ttttataaag
     5581 gaatccggtg tgcgcgccga gatctggtct tatggcattc gtaatccgca aggaatggcg
     5641 atgaatccgt ggagtaatgc actatggctg aatgaacatg gcccgcgcgg tggtgatgaa
     5701 attaatattc cgcaaaaagg caaaaactac ggctggccgc tggcaacctg gggaatcaac
     5761 tattcgggct ttaagatacc ggaagcgaaa ggggagatcg tcgccgggac cgagcaacct
     5821 gttttttact ggaaagattc gcccgccgtg agcggcatgg ccttctataa cagcgacaaa
     5881 ttcccccagt ggcagcaaaa attatttatt ggcgcgctga aagataaaga tgtcattgtg
     5941 atgagcgtca acggcgacaa agtgacagaa gatggccgta ttttaacgga cagagggcag
     6001 cgaattcgtg atgttcgcac tggacccgac ggttatttat acgttctcac cgacgagtcc
     6061 agtggggaat tacttaaagt tagcccacgc aattagctaa cgggaatcat caccactttg
     6121 cgcactgcgg ggcgttcagt gagttgctga taccagcgtt gcagatttgg acgcggtgtc
     6181 caggtcaggc caacgttaaa caaattgtag atgaacggtg cgatagcgat atcgcccaca
     6241 ccaaactcgt cgcctgagaa ccattttact tttgccagtt ccgcatcgag cagggcaaac
     6301 agggcgtcgc actctttgca actggcatca atggcggcct gatcgcgctc ttccggtggt
     6361 gttctgacta atcccatcag gatcccgcga tgagcattac tgagcgtctg gtttgcccag
     6421 tccatccatt tttctgcttc cgcacgacgt gccggtgagt cgatccacag gcgtttttgc
     6481 ccgtactgtg ccgccagata gcggacaatg gcgtttgatt cccaaagaat gagatcactt
     6541 tcgtcgtcac gcaacaacgg caccagcccg ttagggttca tcgccagaaa atcagcatcg
     6601 tgatttatcc caaactcacg gcccgcgaga atttgctcat aaggtagttc cagttcttcg
     6661 agcgtcagca atactttttt tacgttggtt gaattattcc gaccccacag cgtaatcata
     6721 ctcacccctt ttccgacagg cagccacagg ctgaacagga ctccgatggt gaagtaaagt
     6781 taacttttaa gcaacagctg gcaaaaaaat gacaccagaa gcagcgaagc ggcaggatat
     6841 tgcataaact ttaaaaactt taccaactta cggtttcttt aagtttgtgt gtgcgttatt
     6901 aatcaccgaa cttatcatac ggcgatataa cgtatttttt tgaatggata ctcgggtggc
     6961 atttatgacg caatactcct ctctccttcg tggtcttgca gcgggttctg catttttatt
     7021 cctttttgcc ccaacggcat tcgcggcgga acaaaccgtt gaagcgccga gcgtggatgc
     7081 gcgtgcatgg attttaatgg attacgccag cggtaaagtg ctggcagaag gcaatgcgga
     7141 tgagaaactg gatcccgcga gcctgactaa aattatgacc agctatgtgg ttgggcaggc
     7201 gcttaaggcc gataagatta aactcaccga tatggtgacg gtcggtaaag atgcctgggc
     7261 gacgggaaat ccggcactgc gtggttcatc ggtaatgttc ctcaaaccgg gcgatcaggt
     7321 ttcggtggca gacttgaaca aaggtgtgat tatccagtcc ggtaatgacg cctgtattgc
     7381 gctggctgat tacgttgccg ggagccagga gtcatttatt ggtttgatga atggttatgc
     7441 caaaaaactg ggtctgacca acactacctt ccagacggtg cacggcctgg atgcgccggg
     7501 gcagttcagt accgcgcgcg atatggcatt gctgggtaaa gcattgatcc acgatgtgcc
     7561 ggaagagtac gccattcata aagagaaaga gttcaccttc aacaaaattc gtcagcctaa
     7621 ccgtaatcgt ctgctgtgga gcagcaatct gaatgttgat ggcatgaaga caggaaccac
     7681 ggcaggcgcg ggatataatc tggttgcttc ggctacccag ggcgatatgc gtttaatctc
     7741 cgtagtgctg ggggcgaaaa ctgaccgtat ccgttttaat gagtctgaga aattattgac
     7801 ctggggcttc cgtttctttg aaaccgtgac gccaattaaa cctgatgcca catttgtgac
     7861 tcagcgcgtc tggtttggtg ataagagcga agtgaatctc ggggcaggcg aagcgggctc
     7921 agtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agttatacgt taacggaacc
     7981 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggc accattgatt tccagcttaa
     8041 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
     8101 ctttggtcgg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
     8161 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
     8221 atcaacttaa tacgctgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatcg
     8281 ctcacaacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
     8341 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
     8401 ggcaactctt caagattaaa gcaggtagcg cctttactga catgcacgcc cgcagcggaa
     8461 taaaaagcga tatccgggca aaaattattc agcgtttgct gaaaatcgat gggtttgaaa
     8521 atggcgttgc tggcgtgaaa ttcaccaccg caaagaaacg cgcggcaatg gggtttctct
     8581 ttcagcgcca gaaaagtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
     8641 attgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
     8701 tctatcagcg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
     8761 tgatcgctta acaggtaatg gctggcgctg cgtggttcca gaacaatata gccgccgagc
     8821 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
     8881 agcagggcgg cggcgtcttt aagatgtaac ttatcactgc gttttaattc ttgcagcagc
     8941 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     9001 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     9061 aatgcaaaac agaagcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     9121 tgccagataa tctgggcgct aaggcagcca atcatacctg ccagcaatcc accgagcata
     9181 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     9241 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     9301 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     9361 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     9421 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     9481 ccccacaacc aaagtactgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     9541 aaaatcgcca acgagatcat ccacggcgca gagtctggcg ttgcgttaat aagagagaat
     9601 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     9661 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     9721 tttccctcaa gcggcctgtt tacggtaggt gattgtaacg ggcaaaggtt aaataaaact
     9781 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     9841 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ttgcgcgatt attattggcg
     9901 aagaaattgc atgcaaaata aattagcttc cggtgtcagg cttggacgtc aggcgttact
     9961 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgattca
    10021 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
    10081 gatgactgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgttgtcgga
    10141 tcgtattggc cgccgtccgg tgatgttggc gggagtggtg tggtttatcg tcacctgtct
    10201 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
    10261 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
    10321 cgtttgtatc aagatcaccg cgctaatggc gaacgtggcg ctgattgctc cactacttgg
    10381 tccgctggtg ggcgcggcgt ggatccatgt gctgccctgg gaagggatgt ttgtcttgtt
    10441 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
    10501 gcgtataggc gagaaactgt cgctgaaaga actcggtcgt gactataagc tggtgctgaa
    10561 gaacggccgc tttgtggcgg gggcgctggc gctgggattc gttagcctgc cgttgttggc
    10621 gtggatcgcc cagtcgccga ttatcattat taccggtgag cagttgagca gctatgaata
    10681 tggcttgctg caagtgccta ttttcggggc gttaattgcg ggtaacttgc tgttagcgcg
    10741 tctgacctcg cgccgcaccg tacgttcgct gattattatg ggtggctggc cgattatgat
    10801 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
    10861 cgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
    10921 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg ggatgctgca
    10981 aatgctgatc tttaccgtcg gtattgaaat cagcaaacat gcctggctga acgggggcaa
    11041 cggactgttt aatctcttca accttgtcaa cggcattttg tggctgtcgc tgatggttat
    11101 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgattgct
    11161 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
    11221 atcccttcac ctacgctggc aagatggcga acataaggat gcgggcggta agccggagct
    11281 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
    11341 ctgactgaat caggaaccac aaccactttg tatccactcg gcacatcgac ggtgacactt
    11401 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
    11461 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
    11521 tcaggcattg tgttgccggg cgtgctgcct tttttattgc ttaaatggcg cttcgtgttt
    11581 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
    11641 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
    11701 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
    11761 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
    11821 gatgctgccg ttgccggtgt ggaccggcac cataatatcg ccgatggcct catgtaatgc
    11881 tttttgtact tgtggaatca gttcatcgga aagattcaga ccaaacttaa agaagatatc
    11941 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ccgccaccgt
    12001 tttcatggca tcgtcatatt ttttgagggt ataggcgcta ttttttccgc aggcaataat
    12061 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gcaaacgcat ccttcgacag
    12121 ctcgccatta aaaacatctt tgccttcgct cactacccag ccgccgtttt cagccacaaa
    12181 ggctatttca ttagcaattt cagggaagaa agagatcaac tgataatatt gattcccgct
    12241 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
    12301 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
    12361 aatgctcatc aactattctc catcgccgtc tgcgttttgg tatcgggttt ggcgacggct
    12421 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
    12481 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
    12541 gtcgtagcta ccacgctgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
    12601 atagtcagcg ggaagcccaa cgaggcaccc agtccccaaa gtacaacaga caccccagcg
    12661 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
    12721 gcccgaacca cggcaacgcg actgtaacgg tcaatgaacc aaccgccggt aaagcgtcca
    12781 acggtcatcc ccagggtaaa cccggcataa atcagcgagc cggaagtagg gctaaaaccg
    12841 tgaccatcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
    12901 gccagcacca caacaccaat caacagcaac tggatatccc gataaaaagg tacgcctttt
    12961 tcaccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
    13021 gcgatataaa taggtgcgat gcctaccagc gccgccaata aaatatgcac cgttgccgga
    13081 acgccaaagg ccgtaagtgc catcccgaca cctgcgcctg ccagcgtgcc caggctataa
    13141 aaaccgtgca tcatcggtaa aaccgtctta ttcatttctc tctcaacggc ggcaccttca
    13201 acgtttatcg ccacttccgc agaaccaaaa ctagccccaa agacgccgag accaacggca
    13261 aagagcaagg gcgatgtcag ccagagtgcc agacttaata tcatcatccc aatcaatgcg
    13321 caggacatcg tcacaaggat gacattacgc gtcccaaagc gtttcactaa ccacgccgag
    13381 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
    13441 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
    13501 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
    13561 gaagaattta cggtcataga ccacgtcaaa ataagaagag ggaagacaac attagcaaga
    13621 ctgtgtacat ttgtacacaa ttgcagagag aggaaatgac atgcgtcgcg ctaacgatcc
    13681 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
    13741 tgctgttaca caccgcaaaa ttgctaccct tgccggggtg ccgttgggat cgatgaccta
    13801 ctatttttca ggaattgatg agttgttgct ggaggcgttc agccgtttta ctgagatcat
    13861 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctccaggcg catgccaggc
    13921 tatcaccgat atgatctaca gctcacaggt cgcaacgccg gataatatgg agctgatgta
    13981 ccagctctac gcgctggcta gccgaaaacc gctcttaaaa acggtaatgc aaaactggat
    14041 gcagcgcagt caacaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
    14101 tgcgtttatt gagggaatga cgctgcattt tgtcaccgac cgtaagccgc tatcacgcga
    14161 ggagattttg aggatggttg agaggattgc tgggtagtag ataaatttca ggcaacaaaa
    14221 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
    14281 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
    14341 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
    14401 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc ataggtgccc gcatagccca
    14461 gcgccgggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
    14521 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
    14581 acaagaaaca gataaccact ggcacaagac tgacaatcaa tccggcaatc aacatctgac
    14641 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
    14701 taaacaccat taagccgaac tctttcacca tgcttaatgc accctgcgga atgtaaccga
    14761 aggtcgggtg gttagcgcgc ataaatccca gcataattcc ggcgaataac aacccggcag
    14821 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
    14881 caataacaaa gaaggcgcag aatgccagca agtcagtgac ctggctgtga atcgagataa
    14941 agccgatgcg atcggcgatg gtttttacgc gacgggcatc gccgctgact tgtaaaacgt
    15001 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
    15061 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgtttg cccacggcgt
    15121 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
    15181 aaacttcttt gccgttacgg aagctgggat cgagtcgtgc gtgggcgtcg ggatagccta
    15241 ccaacgctat ttcatcgccc atttgtagca cagcatcacc gtctggattc gccagaatcc
    15301 cgttacgtcg aatacgttca atgtagcagc cagtttgtcg ataaataccc agttcacgca
    15361 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcggtaggcg cggatcaccg
    15421 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
    15481 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
    15541 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
    15601 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
    15661 gtgtcgacgt catagagcca gctaacatac cggccgtcag gccaatatcc cagccaaaca
    15721 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
    15781 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
    15841 cgcagaaaat aaacagcata aagccaagat taagcgcgtc ggtgttaatg ctgaaatgtt
    15901 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
    15961 caagtcgtaa tttcccgaga catagcccaa gcgcgaggac cacaaataat aacagaatgt
    16021 aattcccatt taacaattcg gcgacgttta ta
//
LOCUS       DAZFRG010000106        16051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFRG010000106 DAZFRG010000000
VERSION     DAZFRG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.106"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHKUC_002676"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHKUC_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO6618914.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /gene="yicH"
                     /locus_tag="ACHKUC_002677"
     CDS             1447..3156
                     /gene="yicH"
                     /locus_tag="ACHKUC_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6618915.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(3200..3880)
                     /locus_tag="ACHKUC_002678"
     CDS             complement(3200..3880)
                     /locus_tag="ACHKUC_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO6618916.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(3861..4793)
                     /locus_tag="ACHKUC_002679"
     CDS             complement(3861..4793)
                     /locus_tag="ACHKUC_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6618917.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(4841..5701)
                     /locus_tag="ACHKUC_002680"
     CDS             complement(4841..5701)
                     /locus_tag="ACHKUC_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO6618918.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSLVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(5782..6633)
                     /locus_tag="ACHKUC_002681"
     CDS             complement(5782..6633)
                     /locus_tag="ACHKUC_002681"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6618919.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(6645..7736)
                     /locus_tag="ACHKUC_002682"
     CDS             complement(6645..7736)
                     /locus_tag="ACHKUC_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6618920.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(7761..8075)
                     /locus_tag="ACHKUC_002683"
     CDS             complement(7761..8075)
                     /locus_tag="ACHKUC_002683"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6618921.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(8093..8563)
                     /locus_tag="ACHKUC_002684"
     CDS             complement(8093..8563)
                     /locus_tag="ACHKUC_002684"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO6618922.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(8590..10143)
                     /locus_tag="ACHKUC_002685"
     CDS             complement(8590..10143)
                     /locus_tag="ACHKUC_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO6618923.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(10438..12756)
                     /gene="yicI"
                     /locus_tag="ACHKUC_002686"
     CDS             complement(10438..12756)
                     /gene="yicI"
                     /locus_tag="ACHKUC_002686"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO6618924.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWAFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIWQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(12766..14148)
                     /gene="yicJ"
                     /locus_tag="ACHKUC_002687"
     CDS             complement(12766..14148)
                     /gene="yicJ"
                     /locus_tag="ACHKUC_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO6618925.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            14441..14535
                     /locus_tag="ACHKUC_002688"
     tRNA            14441..14535
                     /locus_tag="ACHKUC_002688"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14475..14477,aa:Sec,seq:tca)
     gene            14835..16019
                     /locus_tag="ACHKUC_002689"
     CDS             14835..16019
                     /locus_tag="ACHKUC_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6618926.1"
                     /translation="MTLTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
BASE COUNT         4120 a         4211 c         3776 g         3944 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ttgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggttgttgtc tattcagggc
      241 accagcttca actttgttgc tccgctgatt atgggcggca cagcgctgaa gaccggtggt
      301 gctgatgttc ctaccatgat ggcagctttg ttcggcacgt tgatgctggc aagttgtacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg ttggactgac ctccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggtct tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg tgattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacacaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc ggtttccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtc
      901 tcggcggtat ttaatacttt cccgaactcc tgcttcgggc agaacaacgg ggtgatccag
      961 ttaaccggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gccgggcgat tctgattatc gcgctgtcgc tggcggtcgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgct cctgaatggc tgaaaaacct gctctcctcc
     1261 gggattgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttcaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg
     1501 atcgctggcc tctattttct tctgcaaact cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaacgtcacg tttggccgtg atggccagcc cgcgaccctg
     1681 gtagcaaaaa gtgtcgacat tgcgctaagc agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcaca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgctgacggc
     2101 agctggcaag tggaaaacct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcaccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtc
     2221 atcgacgctc gtttgcaagg tccggactgg gcggtggccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaggaag gtaaactgtc gatgaacgcc
     2341 agcgagttta tttatggctc gctgcattta tttgatccga ttataaacgc ggaattttcc
     2401 ccgcagggcg tagcactgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcaggaaact ggcaacgcga cgggaaaacg ctgatcctcg atgatgcggc aattgccgga
     2521 ctggaatata ccttgcccaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaatct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcaacg
     2761 tttaatcgtg ttgatgtccg tcgtccgtcg ctggcgctaa ctgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt
     2881 tcacaaacgc cacaacgtca gacccatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac attggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gcgatattca ggtcaatgtc ccgctgaaac ctacggttag cgggcaattg
     3061 catgccgtga atgccgcaaa gcagcaagtg actcaaacca tgaatgcggg cgtagtttcc
     3121 agtggtgaag ttacgtcgac ggagccggtg cagtaaaaat aaggacgcta gcggatatca
     3181 gcgctagcgt tccccgttat tagcgcaaaa ttccatactc accgcacaat aaccatttca
     3241 ccggttcatc ttcttctatc gccggaaaac gtcctcccgg ctcaagaaaa taattatcgt
     3301 gttggtcatc atttaccatc gagatttccc agccgagaac aaacgctttt tccgcccaga
     3361 agcgatgata cagacgcggc ggcaggcaaa tgccctgccc cggctttagc accagcgttt
     3421 caccaggttt catggtacgc cgctgaccat caacactcac ggtaatgact cgttgatcat
     3481 cacagagatt ggcttcgttg gcccaggcca actgcatgca taagtctcca ccgccccgat
     3541 tgaggatatc ttccattttg tgggtgtgaa aatgccaggg agtctgctga tcttgttgga
     3601 tctgcaacat tttctcagca tacggttttg gatatgccgc cgaccccagc gcgccgttgc
     3661 gcatcgttag cagtgttagt cccgtttggt tgaaatcacc acgaccaaaa tcggtgatat
     3721 cccaaccgag ctgtaagtcg accacttctt gccagttatc ccgttcccgc tgcagccagt
     3781 catccaccgt aaaataggcg aactctggca aacaaacgcg gaaggtctgc gcaatggcat
     3841 gtgctttatc aataacataa ttaatttgtg aacgtttcat tggcttctcc gttgcgcata
     3901 gcaagccagg cagcgccacg cattccgcta ctgtcgccgt gctgtgcttt tacaatggga
     3961 gtggtgaatt gatcggtgaa aaccagtgga gcgacttctg cgttcagatc ggtgataagc
     4021 agttccacat tcgaaagccc gccgccgatc acaatcacgc cagggtcaac aacgttaacg
     4081 atggttgcca gcgtgcgggc aagctgttgg cgaaaacgtg ccacctgctg catggcacaa
     4141 gcatcaccac gttgtgccag ggtgacaatc gccgcaggag tgagcaagtt accagtcatc
     4201 tgttgatagc gttcactaaa accgctgccg gaaacaaacg actccacgca gttgtgtttg
     4261 ccgcaatagc aactgactga tggaccatct tcctgctccg tatagcctgg caacgtgata
     4321 tgaccgcatt cggcagcatt tccccaggcc cctataaacg gtcgttgctt gatggcaatg
     4381 ccaccgccgc agcccgtacc aagcgtaata ccaaatacca cgtcataatc ttgcccggca
     4441 ccgtcgcaag cttctgatag cgcgaaacag ttaccatcat tggctatcac caccggttgc
     4501 ccaagcaatt gctgcaaatc agcctgtaac gcatgaccgt taatcacctg aatattcgcg
     4561 tttttaatca ggccagtgag tggcgagata ctgccgggta aggcgatccc tgtcagcatc
     4621 ggtcgttgaa cgtcccgcct aatctgctcg ataagcgcca caacgcatga gacaaattgt
     4681 tgatatgtcg acttttgcgt cgggcagcgg taacggcgaa tctcccagcc atgcgcatcc
     4741 atgacaacgg cggcgatttt ggtcccacca atatccaacc ccagatagtg catatctcgt
     4801 tccttgttgc gtaaaaggcg ggtcaccccg cccttctggc ttagtacagt gatgctttac
     4861 cgagcgaacc gaacagtttc attttgtggc gaatgacttc ccgcgccgcg ttaatcgcat
     4921 ccggatagat gacgttcggg tcccaccagc tctctttagc gagaatttca cgcactttct
     4981 ggaaataggc gtatttcata tcgctggaga tattgatttt gccgacgccg agcgtaactg
     5041 attcagcaat ctccgcgtcc gggtttgccg aaccaccgtg tagtaccagc ggaatatcaa
     5101 tgcgtccggc aatgtcgcgc aaaatgtcca tttgcagctt cggttgcatc ccttttggat
     5161 agatgccgtg tgccgtacca atcgctaccg ccagtgtgtc ggcacctgta cgagcaataa
     5221 aatcggcggc ctgcgccgga tctgtgtagg tcacttgtga aacaccacct tccaccgacg
     5281 ttcctgtttg tccgatggtt cccagttcac cctcaaccga tactccaacc gcgtgagcga
     5341 ggcgaaccac ttctgcggtc agcgccacgt tttcttcata cggcagcagc gaaccgtcga
     5401 tcattactga ggtaaatccg cagcgaatcg cacgcaaaac atgctctaca gaggcaccat
     5461 gatcgaggtg cagtacaaac gggaccagac tacgctgggt aatttcgcga acgtgagcga
     5521 aaaaggcatc gcccacaaaa tcatgttcgc tgggatggat ggcaataatc gccggagtat
     5581 ttgtcgcttc tgcttcatcc acgactgcgc gaaggaaaca gttatccgcc acattaaatg
     5641 cgccaatggc aaagccgtgc tggcgggtag gttgcagtaa ttctttcatc gaaactaaca
     5701 tgttaaatct ccagtttcag cgtgtagtcc gccctgccgg tgcgttattg cactggcata
     5761 aaggccatga cagaaaatga atcaggcttt gccgatagcg ccgctcgcct gtaaatacgg
     5821 tgtaatcgct tcttcgaagg ccagttccat agctttcagc gcgtccacca gttcaatgga
     5881 tatatcgtga cagagggtat gcaggagcgc ggatttcccc cgttcaaaaa tggccgcccc
     5941 cacgttgatt tttgctaccc ccagcgtcgt acagagctgg agctgatcga acggcgtacc
     6001 gctaccgcca tgtaatgcca gaggcacgct cgagcagcgc gaaatctccg ccagacgctg
     6061 aaaatcaata gcaggtacaa cgccaggcgc gtacatgccg tgcgccgtgc ctaccgagac
     6121 ggccagtaga tcaatgcctg tctgcgcgat aaacggttcc acatcagtgg gttgcgtcat
     6181 caggtctgca ttggcggcct ggtcgtaacg cggcgcatct gccagatggc ccagttcgcc
     6241 ttccacgcag agctgcacgc tggcggcgag ttccaccacc gccctggtct ggcgcacgtt
     6301 ttcctccagg ggaaaagcag aagcatcaat cattaatccg ctgaaaccag cacgaaacgc
     6361 ctgacgaata cagtccggat ttttgccatg atcaagcgcc agagcgaccg gtactgaggc
     6421 agattccgcg agcgttttaa ccactgccgc cgcgacgggt gcaggaaaat gcgccagatg
     6481 cccctgatag agattgagaa tgatcggagc gcgattcttc tccgccacgc ggatagctgc
     6541 gtgagcactt tccagattca tacagttgat cgccagtaag gcgtaatgtt catgccaggc
     6601 tttcatcacc atacttttca tatcggcaaa catagggcct ccggttattc gagggtgaaa
     6661 gtgatctctt catctttcag cttttgatcg ctcagttcgt caaactcttc gtcacgttcc
     6721 gttaccggtt tcttccagag tgaaagcatc acgccacaaa tcaccgtgcc tatacccagc
     6781 gccaggcaaa acagcagagg atgggtgaac agaggcacaa caaacatgcc gccatgcgga
     6841 accggactct ccacgcccca caccatgatt aagccgcccg cgaccgccga tgccaccaca
     6901 caagatccca ctactcgcag caggtcacgc gcggcgatcg gaatgactcc ttcagtgatc
     6961 atgcagatcc ccatcggaaa cgcggctttc agcgcctcgc gctccgcacg ggtgtattta
     7021 tggcgtgtca gtaagaacga aagtgtgatg ccaaacggag ggatgattga gccgacaaat
     7081 ttcacggctt ctggtccgta gacgccactg accagcaagc catcggaaaa cagcgacatg
     7141 gttttgttga ccggaccacc gaaatcgaag gttgccatcg cgccaagtat ggcccccatc
     7201 aggaagcgcg atccgccctg catcgactcg agcaaatgaa tcagtgcttc ttgtaaccag
     7261 gcgatgggtt taccaatcag cgtcatcatt aacagcccac cgataatggt gcttaatacc
     7321 ggcaacacca tgattggcat gagtccttgc atcgactgcg gcaggcggat atagcgttta
     7381 agtagcaaaa tggtgtagcc caccaggaaa ccacccaaca taccgccaat aaatccggta
     7441 tggatttgcc cgcagagaaa gccgacaatc aggcccgggg caaatccggg acgatcggca
     7501 atagaatagg caattgccgc gctaatcagc ggcacaatca gccccattcc ccagccgcca
     7561 atctgattaa gcatccacgg gattgttcct gttttttcac caacatctgt accgccaaga
     7621 acttgcccca acgcaataca tatcccggcg gccacaataa ggggtatcat ccacgagatg
     7681 ccggttaata aatgtttttt aatttcctgt gaaaccggaa ttttattgtc tttcatatta
     7741 agatccctcc agtataagga tcatttgttt cccagtgatt tttcgacctt ttcaagaaat
     7801 tgaacgggcg atttaatgac gatctctgtc tttactcgca caatacgttt attggtgaag
     7861 cgttcttctc cttttatttt cacatctaca gcaagaatca cgacgtcagc agcagagata
     7921 tctgccgcct gtaattcatt ttctgtacca atggttccct gggtttcaac tttaatggta
     7981 tggcccagag cttttgctcc tttgattaac ttttcacgag caatataggt atgagcaata
     8041 cccgccgtac aggcggcaac acagacaata ttcatgacat acttcctgtt atttagcaga
     8101 catcggattg tgcatactga ctaaagagat caatgatttt ttgcggactc gtctcaacta
     8161 ataattgttc tatgatgtca tcatccgcca gcgcgcccgc gacctgggaa agtaaacgga
     8221 tatgggtggt attttgatct tcaagacgta ccgcaaataa aataatacag cgcacgctac
     8281 tgccatccag cgtttcccag ggaatatcct ggcgggtacg gccaattgcc agcgtagtat
     8341 taattaccgc agaagattta ccatgcggga tcgcaatatg attttcaaaa ccagtcgagc
     8401 cttccgcttc gcgctgccag acatctttaa taaaggtttc ccgacaatcg atcgcaccat
     8461 cttttttgag aagattagtt aattcttcaa tcgcctccgc tttattcgtc gcttgcatat
     8521 ctaaaataac gcgattcgta ttcaggattt ttgttatgtc cattcgtcat acctcataaa
     8581 attctgacat taaatttcaa cgacccgaga cgtcgcatga tgtaattttt ccgtaatcac
     8641 catttcagtg acccgcttaa tatcggcatc attaaaaaaa gcggtaacat aagcgaccgg
     8701 catagtgact gtaggcaaat taatggtcga aataatcagt tcgatattgt caggcaaaac
     8761 ctgtgataaa ttcgctgctg aaataacatc aacaatcgtc cattcaggaa atgctcgcag
     8821 aatacggctt ttcagtagat gcgaagttcc gattccggtt gaacagacca gtaatacacg
     8881 tttacgggca atttgccgct ccatcgcagc ctgaaaatga acggtcaggt aacccacttc
     8941 atcctcgctc actgcattct caccccaggt tttaaatacc tgattcacca catattgcgt
     9001 caaccgccag acatccgcta attctgcttt aatatcttcc agcaatggat tacggatatg
     9061 aatgcgataa tttagacggt taatcagcgg cttaatatgc accaataagc catcatataa
     9121 tgcgctgtct tcactaaaat cgcagtccac gatgtcactg aataccgtaa ttaaacgcca
     9181 ggtaatcaga cgcgcttcat tgctggcctg catatgtgaa ataatgctaa catctttttg
     9241 tccatcaatc gccacacctg atgaaatgat atattgataa ataaaccaga cttcatcatc
     9301 gggcaatgta tgtgcaattc gttgttctat cttatgaatc atctgactgg caacattaaa
     9361 gatattttca tcatattgtc tggtttgatc ttcttctctt gataacgcat tcccgtgcgt
     9421 gttgcgatac atcataataa ggatgtgagt aaaaatgtta acgtaataag gttctcccaa
     9481 agaccaactt aattcatttt ccatatccag taacaatgac tggacaaata acacctcttc
     9541 ttctccaaaa taatggacta atgctttata gcttccggga tccagacgtg aatacacgac
     9601 accttgcggc tcattatggt taataatgcc gttaatcagt aatgccattg cctgtcggac
     9661 ttgcccttca ctaccttcaa tatgcgtacc acttggactg cggatcaacg ataaccccaa
     9721 cggcgcaagc caggactcaa ttactctcag gtcattaaca atagaggctc cgctgataaa
     9781 gtagcgttct gacaatttac tgatcgacgt ttcattcggt gtttcactta ataactgcga
     9841 cgctatttta acgcgcctgg cattattcat tacaccatca atctcttcac attctgccac
     9901 caacaaatga tcaagatgca gcaaatcagc aatattctcc gcgcttaaca tcacacccgc
     9961 gccagcacgc ttctcaacat ttattctcca ttgtcccagc cattgctcaa gtaaacgtaa
    10021 atcacgttga atggtttttt ccgaaacatt taaatagccg gcaatcgtaa caatagtcgt
    10081 atattccctt cgaggtaaaa ggaatcgtaa taatctgttt tgtcgcgagg taatcatttg
    10141 cattttttac ccttatcaca gattaagaag ccccatatgg aagagcttca gttgccatct
    10201 ctgagggtaa atataaagca gaataatttc acaacaatac taaaatctgt ccgtaatccg
    10261 gacagtgata aaacaaagca aggcggttaa gggatatttt tcagcacaga tcacattttc
    10321 gtgatgaata atcgtgatca tgctcgcaaa tacgatggaa gaaatattta ttaccagatg
    10381 tgagattatt taccggatac gacacaaaac cattcgtatc cggcattctt caatagatta
    10441 caacgtaatt gtcagcgcat tcccttgagg agtcaccacc agtccctgct cactttcagc
    10501 ctgcgaaccg ccttgcagac catttacttt cacaacattg cgcaagcaca gtgtccagtt
    10561 cttcgcctcg cccgtaccag tcacagtaat cgtgttgcca gtacgtgctg cttttaaagt
    10621 aaagatcacc gaaccgtcag cagtgggcac ttcacagaca gcctcatgcc cgtcttgcag
    10681 attaaagagg tggaatgccg taccttcgtg ccacgcgtaa tcgggacgtt gatcgttgtt
    10741 gcccagcgcc agtagggtgc tatcacgcac ataaacgggc agactcagga agctgtgctg
    10801 ctgtttatgc cagcgactac cgtcgagttc atcgttgtgc cacaggtgtg tccagcgacc
    10861 ttccggcagg tagaactgca catcgcccgc ttcagtgaac acaggcgcaa ccatcacgtt
    10921 gtcgcctaac atgtattgac ggtcaaggta atcacaagcc ggatcgtccg ggaactccat
    10981 catcatggcc cgcatcatcg gcgtaccccg ggcgtttgca cgtgcagctt cacgatacag
    11041 atacggcatc atgcggcatt tcagttgcgt gaagaagcgc accacatcac aggactcatc
    11101 atcgtaggcc cacggcacac gataagattt gctgccatgt aaacggctat ggctggaaag
    11161 caaaccaaac gcgcaccagc gtttgtaaac gtgcgccgga gcggtatttt caaagccgcc
    11221 gatatcgtgg ctccagaagc caaaacctga aaggccaata gacaaaccac cgcgcaggct
    11281 ttccgccatt gattcgtagt tagcgtaaca gtcgccaccc cagtgtaccg gaaatttctg
    11341 cgcaccaacg gaggccgagc gggcaaacaa gacggcttct tcctcaccaa cggtgtcctt
    11401 gagcacgttc cacaccagtt cgttgtagat gtacgcataa tggttatgca ttttctgcgg
    11461 atcggaacca tcaaaccact gaacatcggt tggaatacgt tcgccaaagt cggtcttaaa
    11521 gcaatcaacg cccatcgcga ccagaccttt cagtttgtcg gcgtaccatt tgcaggcatc
    11581 cggattggta aagtcataaa tcgccagacc tggctgccat ttatcccact gccacagcga
    11641 accgtccggg cgtttgagta aataaccttt ctcttgtaac tctttaaaga cgggggattt
    11701 ttgccaaata taggggttaa tccagacgca gattttcagc cctttcgctt tcagacggcg
    11761 gatcatccct tccgggtccg gaaaagtcag cgggtcccac tcaaaatcgc accactggaa
    11821 ggctttcatc cagaaacagt caaagtggaa aacatgcagc ggcagattgc gttccgccat
    11881 accatcgata aagctgttta ccgtcgcttc gtcgtagttg gtggtaaatg aagtggttag
    11941 ccacagaccg aaggcccacg cgggcggcag cgccggacgg ccagtaaaac gggtataacg
    12001 atcaagtacc gctttcggcg tcgggccgtc gataacaaag tattcgagat attcactctc
    12061 aacgctgaac tgtactttgg agactttctc cgatcccact tcaaaagaga cgcattgagg
    12121 atgattgacc agtaccccat aaccacggtt agtcatataa aacgggatat ttttatacgc
    12181 ctgttcagta cttgtgccgc cgtcccggtt ccaggtctct accgtctgac cattgcgcac
    12241 cagggcagta aagcgctctc ccagaccgta aactgtttcg ccaacgccaa gatccagccg
    12301 ctcaaacata taatttcgtt gattattcgt gtcctgcacg tagccgttat ttttcacctg
    12361 actaccggta atacgttcgc cgttgcgcag aaaatccagt gaccagaact cacctttgct
    12421 gacacgcgca cttaagttgc cacttttaaa ctcagcgtaa cgttctgtgt tttcgatagt
    12481 gaccttcacg tcctgcaaaa tattgagcgg gtaatgagga ccgttattca gcgccccctg
    12541 aaaatgctca atccgtacac cgacaatacc ttcctgtggg gagaaaaagc gcagcgtaaa
    12601 taaaggcgta tcaagctgcc aggtacgttc acgcacatca cgaggggcag catagaccac
    12661 catttcatta ccctgctgtt caacctcgaa cacctgaagc gggtgaatca aattgaggcc
    12721 aggttgaatc aaccagtttc cgtcgctaat tttcattgcc gttccttagt tctgcacttc
    12781 ttgagaggtg aattgttgct ggcaacggcg tttgccctgc gccagctgtt ccataacggt
    12841 tttcagattg tgcgtcgtga gcgagtagta gcgtttagcg ataatcgcgc tcagcaagta
    12901 acagatcgcc ggaacaatcg tgaatagcgc gataatgatg ctaattgtga cgctgttctg
    12961 cgctttttct gccgcatcat atccgccata agccagcatc cagccgataa gggcaccacc
    13021 gaaggccaac cccagtttga gcacaaacag cgtgccagca aaactgatcc cggtcaggcg
    13081 tttaccattg caccactcgc cgtagtcgac ggtatcggac atcattaccc actggatagg
    13141 tgtcaccagt tgatgcaaca caccaatcac gaagatgaag acaaacatgg tgatgctggc
    13201 ctgcatagga acaaagaaca tcgcgaggct aatcactgcc agcagggcgt tcgtccacca
    13261 gaagatagtg actttacatt tccagtcggt cagcggtttc gccagtgcgg aaccaatcag
    13321 gttacccacg caataagtgg tgagaaaagc gacaaatact tccggcgtgc ccaaaatcca
    13381 tgtgacgtaa tacatcatcg ccccaccgcg tacgcacacc gccaggatat tgaaaatggt
    13441 caggaaaccg acaatacgcc actggtcgtt ttgccagata tcacgtaaat catcccgcat
    13501 agacgtcgtt gtaggtggtg cttcaacgcg ttctttagtg gtgaagaaac agaatgccag
    13561 catcataaat gccaccacgg aaaggaccgc gataccgccc tggaaaccga gaggtttatt
    13621 atcaccacca attaaattaa ccagtggcat catcagaaca gtagagagca tgcctcccgc
    13681 ggtcgccagc acaaaacgcc aggattgcag cgagatacgc tgagtcgggt cattggtgat
    13741 tacaccaccc aatgcgcagt aagggatatt gacgatggta taaagtaagg taagtagggt
    13801 gtaagtaatt gctgcataaa tcatttttcc gttcatactg agatctggcg tgctataggc
    13861 cagtacacag acgatcccga atggcagtgc gccaaacagc acccacggcc gaaatttacc
    13921 ccagcgagag cgcgttcgat cggccaacaa ccccatgcaa ggatcggaaa tcgcatccag
    13981 tgcacgagcg accaaaaaca tggttccgac aaatccggca ggaatgccaa aaatatcggt
    14041 ataaaagaac atcatatata acattacgtt atcgaaaata atgtggctgg cggcgtctcc
    14101 catgccataa ccaattttct ctttaacgga caacacttca ctcttcatct gcttttcctt
    14161 gatgctatag gggtgctgag accgataacg cattttgtaa accatccctg cgagagcgaa
    14221 tattccgata tctggttatt aatttatgat tcttgtttta tgtgatcgtg gtagcgttaa
    14281 tttcgctcat atatcattgt aaaatatgag ttttatatga actataatgt tttcgtgata
    14341 atacgctgcg tgtattaggc ggaaaaaact gatctggggg atgtagaaac tcaaggaagt
    14401 agctataatg cgccccgcct ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt
    14461 gaggcggctg gacttcaaat ccagttgggg ccgccagcgg tcccgggcag gttcgactcc
    14521 tgtgatcttc cgccagttta cctcttctga actctcccaa tgtcaataaa tctcttctaa
    14581 tcccttgtat atcagtattt atttgtatcc tgaggtcaac ctacttcacc ccgtatcaag
    14641 tttcttttgg gggtacttta gggggtgtta tttcctattg aaatacaagc acccccataa
    14701 taggaaaaat gtgatacata acaacggctt ttaacaacat ctttgttata caaggggtgt
    14761 attgggggta tcaggttagt tactggcaga aatccccctc aggaacatga attcatcaaa
    14821 agccggagga tgtcatgaca ttaaccgata tcaaagtgaa aaccgcaaaa cccaaagaca
    14881 aaccctataa gcttgctgat ggcggcggta tgtatctact gattaacact aatggttcta
    14941 aatactggcg catgaagtac cgctttgctg gtaaagaaaa gatgctttct attggcgtat
    15001 accccgatgt aacgctagcc gatgctcgcg agaaacgcag tgaagccagg aaactcctcg
    15061 ctgctggagg cgatccaggg gaggcaaaaa aggaagaaaa aatcgcgcag caaataagtc
    15121 tgaaaaatac gttcgaggct attgctcgcg aatggcacca gtcaaaagcc gatcggtggt
    15181 cattacgcta tcgtgatgaa atcattgata cctttgaaaa agacattttt ccctacattg
    15241 gcaagcgccc tattgccgaa atcaaaccaa tggagttact ggaagcactg cgtaaaatgg
    15301 aaaaacgtgg agctctggag aaaatgcgca aggtccgcca gcgctgtggt gaggtgttcc
    15361 gctatgcaat tgttactggc cgggcagact ataacccagc ccccgatctt gccagtgctt
    15421 tagctacacc gaaaaaagta cattttccct ttcttactgc taatgaactt ccccacttcc
    15481 tcaacgatct ggcgggttat accggaagta taatcactaa gacagctact cagatcatta
    15541 tgttaaccgg cgtacgaaca caagaactgc gttttgcacg ctgggaagat atcgattttg
    15601 agacaaaatt gtgggagatc ccggcagaag taatgaagat gaagcgacct catattgtgc
    15661 cgctctctga gcaggttatt atgctattta agcagcttga accaatctca aaacatcatc
    15721 ccctagtctt tatcggtaga aatgatcccc gcaaaccaat tagtaaggaa agcattaatc
    15781 aagtaattga attacttgga tataagggaa gactcacagg gcacggtttc agacacacca
    15841 tgagtacaat tctgcatgaa caaggcttta actccgcctg gattgaaatg cagttagctc
    15901 atgtggataa aaattccatc aggggtacct ataatcatgc cctgtatctc gatggccgcc
    15961 gtgaaatgat gcaatggtac gctgattaca ttgattcact ctccagccgg gaaagttaag
    16021 tcagaaatac ttttccctga tgttttcctc a
//
LOCUS       DAZFRG010000107        15949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFRG010000107 DAZFRG010000000
VERSION     DAZFRG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.107"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..1862)
                     /gene="typA"
                     /locus_tag="ACHKUC_002690"
     CDS             complement(39..1862)
                     /gene="typA"
                     /locus_tag="ACHKUC_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO6618927.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2235..3644
                     /gene="glnA"
                     /locus_tag="ACHKUC_002691"
     CDS             2235..3644
                     /gene="glnA"
                     /locus_tag="ACHKUC_002691"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO6618928.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3818..4867
                     /gene="glnL"
                     /locus_tag="ACHKUC_002692"
     CDS             3818..4867
                     /gene="glnL"
                     /locus_tag="ACHKUC_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO6618929.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DLSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4879..6288
                     /gene="glnG"
                     /locus_tag="ACHKUC_002693"
     CDS             4879..6288
                     /gene="glnG"
                     /locus_tag="ACHKUC_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO6618930.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6400..6510
                     /locus_tag="ACHKUC_002694"
     CDS             6400..6510
                     /locus_tag="ACHKUC_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO6618931.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6743..8116)
                     /gene="hemN"
                     /locus_tag="ACHKUC_002695"
     CDS             complement(6743..8116)
                     /gene="hemN"
                     /locus_tag="ACHKUC_002695"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO6618932.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8305..8814)
                     /gene="yihI"
                     /locus_tag="ACHKUC_002696"
     CDS             complement(8305..8814)
                     /gene="yihI"
                     /locus_tag="ACHKUC_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO6618933.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8933..9099)
                     /locus_tag="ACHKUC_002697"
                     /pseudo
     CDS             complement(8933..9099)
                     /locus_tag="ACHKUC_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9396..10028
                     /gene="yihA"
                     /locus_tag="ACHKUC_002698"
     CDS             9396..10028
                     /gene="yihA"
                     /locus_tag="ACHKUC_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO6618934.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10410..13196)
                     /gene="polA"
                     /locus_tag="ACHKUC_002699"
     CDS             complement(10410..13196)
                     /gene="polA"
                     /locus_tag="ACHKUC_002699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO6618935.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13560..14492
                     /locus_tag="ACHKUC_002700"
     CDS             13560..14492
                     /locus_tag="ACHKUC_002700"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6618936.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQEK"
     gene            complement(14533..>15949)
                     /locus_tag="ACHKUC_002701"
     CDS             complement(14533..>15949)
                     /locus_tag="ACHKUC_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6618937.1"
                     /translation="ATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKKKGEHA
                     YDMMLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPITMLMH
                     GNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGK
                     FSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLEEKKFP
                     VGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQ
                     LGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYL
                     PLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFNMKLPLNV
                     ATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMLQLITIDNNTASLQLRYTPGKV
                     FFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3753 a         4075 c         3957 g         4164 t
ORIGIN      
        1 ccatcgctgg caggtttttt tatgactaaa aaacgaaatt aatcgtcttt cggtgcgcgg
       61 ttggcgcgac gacgatcgtt ttccgtcagg tgacgtttac gaatacggat agaggtcgga
      121 gtcacttcta ccagttcgtc atcatcgatg aactccagag cttgttccag agtcatgcgg
      181 ataggcggaa ccagaacaac ggcttcgtca gtaccggaag cacgcatgtt ggtcagtttc
      241 ttaccggtca ggcagtttac agtcaggtcg ttagagcggc tatgaatacc gataatctga
      301 ccttcgtaaa cttctgcacc gtgaccgagg aacagcttac cacggtcctg cagaccgaac
      361 agcgcgaacg cgaccgcttt accctgaccg ttagagatca gtacaccgtt ctgacgctga
      421 cccacttcgc ccggacgtac gtcgtcgtag tggctgaagg tggagtacag cagaccagta
      481 ccggaagtca tggtcatgaa ctcagaacgg aagccaatca gaccacggct tgggatcacg
      541 tagtcgagac gtacgcggcc tttaccgtct ggattcatgt ttttcaggtc gcctttacgt
      601 tcgcccagcg cctgcatcac agaaccctga tgctgttctt caacgtccag cgtcacgttt
      661 tcatacggct cttgtttacg accgtcgatt tcacggaaga ttactttcgg acgggatacc
      721 gccagttcga aaccttcacg acgcatgttt tcgatcagaa cagacaggtg cagttcgcca
      781 cgaccagaaa cgcggaacgc atcggcgtct tcggtttctt ctacgcgcag cgcaacgttg
      841 tgtaccagtt ctttgttcag acgatccagg atctgacgag acgttacgaa cttaccttct
      901 ttaccgcaga acggcgaggt gttaacgcag aagaacatag aaacggtcgg ctcatcaacg
      961 gagagtgccg gcagcgcttc aacgttttgc gtgtcgcaaa cggtgtcaga aatgttcagt
     1021 tcgccaaggc ccgtgatcgc cacgatatcg ccagcttccg ccagatcggt ttcgatacgt
     1081 tccagaccga ggtggcccag cactttaccg actttcgcgt tgcgggtttt gccttcgcta
     1141 tcgatgatag taacctgctg gttcggcttc actttaccgc gcttgatgcg gccaatgccg
     1201 ataacgccaa catagctgtt gtaatcgagc tgagaaatct gcatctggaa cggaccgtca
     1261 aggtcaacgt ccggcgcagg aacgtggtca acaatcgcct ggtacagcgg ggtcatgtct
     1321 tccgccatat cttcgtggtc cagacccgcg ataccgttca gcgcagaagc gtaaacgatc
     1381 gggaagtcca gctgctcgtc ggtcgcgtcg aggttaacga acagatcgaa tacctgatcc
     1441 acaacccaat caggacgcgc gccagggcgg tcaactttgt tgataacaac aatcggcttc
     1501 aggccgtaag caaacgcttt tttggttacg aagcgcgttt gcggcatcgg gccgtcaaat
     1561 gcgtcaacca ccagcagcac tgagtctacc atggacatta cacgttcaac ttcaccaccg
     1621 aagtcggcgt gccccggggt atcaacgatg ttgatacggt aatcattcca tttgatagcg
     1681 gtgtttttcg cgaggatggt aatcccacgc tctttctcca aatcgttgga gtccatcacg
     1741 cgctcttggg tttcggcacg agagtcgaac gtaccggatt gttggagcag cttgtctacc
     1801 agggtggttt taccatggtc tacgtgcgcg atgatggcga tattacgcaa tttttcgatc
     1861 acaactttgc ctcaggcatt agaaatagcg cgttattgta cacggattaa tcgcactaca
     1921 aaacaggatc acaaacatcc tccgcaaaca agtattgcag agtccctttg tgatcgcttt
     1981 cacggagcat aaaaagggtt atccaaaggt cattgcacca atatggtgct taatgttccc
     2041 attgaagcac tatattggtg caacattcac atcatggtgc agcccttttg cacgatgtta
     2101 agcatgataa cgccttttag gggcaattta aaagttggca cagatttcgc tttatctttt
     2161 ttacggcgac acggcccatt taattgcaga tttcgttacc acgacgacaa tgaccaatcc
     2221 aggagagtta aagtatgtcc gctgaacacg tactgacgat gctgaacgag cacgaagtga
     2281 agtttgttga tttgcgcttc accgatacca aaggtaaaga acagcacgtc actatccctg
     2341 ctcatcaggt gaatgccgaa ttcttcgaag aaggcaaaat gtttgacggc tcctcgattg
     2401 gcggctggaa aggcattaac gaatccgata tggtgctgat gccagacgca tccaccgcag
     2461 tgattgaccc gttcttcgcc gattctaccc tgattatccg ttgcgacatc cttgaacctg
     2521 gcaccctgca aggctatgac cgtgacccgc gctctattgc gaagcgcgct gaagattacc
     2581 tgcgttctac tggcattgcc gacaccgtac tgttcgggcc agaacctgaa ttcttcctgt
     2641 tcgatgacat ccgtttcgga tcgtctatct ccggttccca cgttgctatc gacgatatcg
     2701 aaggtgcatg gaactcctcc acccaatatg aaggtggtaa caaaggtcac cgtccggcag
     2761 tgaaaggcgg ttacttcccg gttccgccgg tagactcagc tcaggatatt cgttctgaaa
     2821 tgtgtctggt gatggaacag atgggtctgg tagttgaagc tcatcaccac gaagtcgcta
     2881 ccgcaggtca gaacgaagtg gctacccgct tcaataccat gaccaaaaaa gctgacgaaa
     2941 ttcagatcta caaatatgtc gtgcacaacg tggcgcaccg tttcggtaag accgcgacct
     3001 ttatgccaaa accgatgttc ggtgataacg gttccggtat gcactgccac atgtcgctgt
     3061 ctaaaaacgg cgtcaacctg tttgctggcg acaaatacgc aggtctgtct gaacaggcac
     3121 tgtactacat tggcggtgta atcaaacacg cgaaagcgat taacgccctg gcaaacccga
     3181 ccaccaactc ttacaagcgt ctggtcccgg gctacgaagc accggtaatg ctggcttact
     3241 ctgcgcgtaa ccgttctgcg tctatccgta ttccggtggt ttcttctccg aaagcacgtc
     3301 gtatcgaagt acgtttcccg gacccggcgg ctaacccgta cctgtgcttt gctgccctgc
     3361 tgatggccgg tcttgatggc atcaagaaca agatccatcc gggcgaagcc atggacaaaa
     3421 acctgtatga cctgccgcca gaagaagcga aagagatccc acaggttgca ggctctctgg
     3481 aagaagcact gaacgaactg gatctggacc gcgagttcct gaaagctggt ggcgtgttca
     3541 cggatgaagc gatcgatgcg tacatcgctc tgcgtcgcga agaagatgac cgcgtgcgta
     3601 tgactccgca tccggtagag tttgagctgt actacagcgt ctaatagttg aagttgtact
     3661 acccggcgca acaacgccgg gatttagttg ccgtggaaac tttcagccca tctctgcatg
     3721 ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa aagctataat
     3781 gcactaaaat ggtgcaacct gttcaggaga ctgctttatg gcaacaggca cgcagcccga
     3841 tgctgggcag atcctcaact cgctgattaa cagtattttg ttaatcgatg acaatctggc
     3901 gatccattac gccaaccccg ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt
     3961 tggtacgccg ttaccggaac tgttgagcta cttctcatta aatatcgagc tgatgcaaga
     4021 aagtctggag gcggggcaag gttttaccga taacgaagtg acgctggtca tcgacgggcg
     4081 ctcgcatatc ctttctgtga cggctcagcg tatgccggac ggcatgatcc tgctggagat
     4141 ggcaccgatg gataaccagc gccgcttaag tcaggaacag ctacagcacg cccagcaggt
     4201 tgctgcccgt gatttagtgc gcggcctggc acatgagatt aaaaatccgc ttggcggttt
     4261 acgtggcgcg gcgcagctgc tcagcaaagc attacctgac ctgtcactac tcgaatatac
     4321 caaagtgatt atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc
     4381 gcagctgccc ggtacgcgca ttaccgaaag tattcacaaa gtggctgaac gcgtggtgac
     4441 gctggtgtcg atggaactgc cgaacaacgt gcggttgatt cgggattacg accccagcct
     4501 gccggaactg gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa
     4561 tgcgctacag gcgctggggc cggagggtgg tgaaatcatt ctacgtaccc gcacagcgtt
     4621 tcaactgacc ttacacggcg agcgctatcg gctggcggcg cggattgatg tggaagataa
     4681 cgggccgggc attccgcctc atttgcagga tacgctgttt tacccgatgg tcagcggccg
     4741 cgaaggtggc accgggcttg gcttatccat cgcccgtaat ttgattgatc agcattcagg
     4801 caaaattgaa tttaccagtt ggccaggtca taccgagttc tcggtttacc tgcctatcag
     4861 gaaataaagg tgacgtttat gcaacgaggg atagtctggg tagtcgatga cgatagttcc
     4921 atccgttggg tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac gacatttgag
     4981 aacggcgcgg aagtactgga ggcgctggcg agcaaaacgc cggatgtgct gctttcagat
     5041 atccgtatgc cgggaatgga cgggctggcg ctgctcaagc agattaaaca gcgccatccg
     5101 atgcttccgg tcatcattat gaccgcacat tccgatctgg atgctgccgt cagcgcctat
     5161 caacaagggg catttgatta tctgcccaaa ccgtttgata tcgacgaagc cgtggcgctg
     5221 gttgagcgcg ccatcagtca ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac
     5281 ggcccaacga ccgatatcat cggtgaagcg ccagccatgc aggacgtgtt ccggattatc
     5341 ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg caccggtaaa
     5401 gaactggtcg ctcatgccct gcatcgccac agtccgcgag ccaaagcgcc gtttatcgcg
     5461 ctcaatatgg cggctatccc gaaggatttg atcgaatccg aactgtttgg tcacgagaaa
     5521 ggcgcattta ccggcgcgaa taccattcgt caggggcgtt ttgaacaggc tgatggcggt
     5581 acattatttc tcgatgaaat tggcgatatg ccgctggatg tgcagacgcg tttgctgcgc
     5641 gtgctggcag acggtcagtt ttatcgcgtt ggcggctatg cgccggtgaa agtagatgtg
     5701 cggattatcg ctgccactca ccagaatctt gaacagcggg tgcaggaagg caagttccgt
     5761 gaggatctgt tccaccgcct gaacgtcatc cgcgttcatc tgccgccgct gcgcgaacgt
     5821 cgggaagata ttccccgtct ggcgcgccat tttttacagg ttgccgcgcg agagctgggc
     5881 gtagaagcga agttgctgca tccggaaacc gaagccgcgc tgacgcgtct ggcgtggcca
     5941 ggcaacgtgc gccagctgga aaacacctgt cgctggctaa cggtgatggc cgccgggcag
     6001 gaagtgttga ttcaggattt gcctggcgaa ctgtttgaat caaacgtacc ggaaagcact
     6061 tcacacatgc agccggacag ttgggcaacg ctgttagcac agtgggcaga cagagcgctg
     6121 cgttccggtc atcaaaacct gctttccgaa gcacagccag agctggaacg gacgttactg
     6181 accaccgcgc tgcgacatac gcaggggcat aaacaggaag cggcgcggct acttggctgg
     6241 ggtcgcaaca ccctgacgcg taagttaaaa gagctgggga tggagtgatt cacggcttgt
     6301 gtgtaaagat tgattattga gcgcaaattg ctggtatttt gcgctttact gttccgataa
     6361 gttcagtatg atcttgcccg gaaaacgggg agagtcatta tgctggaatc aataattaat
     6421 ctggtatcga gtggcgcagt tgacagccac acaccgcaaa ctgctgttgc tgccgtgctg
     6481 tgtgccgcga tgattggtct gtttagctga agctcgttgt ttacgccgga tgcggagaaa
     6541 gtcgtaggcc tgataagacg caaccagcgt cgcatcaggc gttgattgcc ggatgcggcg
     6601 taaacgcctt atccggccta caaaagcaat aatttcaaca gattttgcag gcctgataag
     6661 cgtagcgcat caggcaatct tgttctcagc acattgatgg cgctttcgtt ttctactttg
     6721 taaacgaagc gccattcaca atttaaatca cccgagagaa ctgctgcatc cgcgctttct
     6781 ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga cctttcgccg
     6841 tcacctgtat tcccttctca tccacatcca ccagcccatc ttttgctaac ggggcgagca
     6901 gcttgagatc ttccgcaaag taatcagcga agagcaaatc ccactgttgc tcaatagggg
     6961 cgtaatccag acggaagttg cagatgagcg acttaatcac atcgcggcga atacagtcat
     7021 cacgcgttag cgcaatacca cgccacagcg cattgccttg ttcatccact tgctgatagt
     7081 actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg atggcggaaa
     7141 cgcccatccc aagcagatcg gtatcgccct gggtggtgta gccctggaag ttacgatgca
     7201 gcacgccttc acgctgggcc accgccagct cgtcatccgg acgggcaaag tgatccatac
     7261 cgataaactg atagcccgat tgtgtcagga aggcgatggt ttcctgcagg atatcgagtt
     7321 tttgctgcgg actcggcagg tcagcgtctt tgattttgcg ctgagcagca aaaatggtcg
     7381 gcaaatgcgc gtagttaaag acgctcagac gatcggggtt cagctccgcc acgcgtttca
     7441 gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc gtaaatcagg tcgatgttgg
     7501 tggaggtaaa gccaatctca cgcgcatggt taagcagtgc aaagatgaac tcttcatcct
     7561 gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc atgctcagcc
     7621 gattaaagcc ttcggcgcgt aaatgatcga gtacatccag ttcgatttcc cgcggatcga
     7681 cttcgatcga gatctccgca tcggcattga actggaagtt ttcgcgcagc agcttcatca
     7741 gacggctgat ttgcgcttta ttcagatacg tcggcgttcc gccgccccag tgcaattggc
     7801 tgacgtgacg cccggcgaac agcggtgcac gatggacgat ttcttgctcc agcgcgtcca
     7861 gatactgatc ggccttgtgc tgctggcgag taacaatctt attgcaaccg cagaagtaac
     7921 aaagcttatg gcagaacgga atatgtacgt agagagataa tggacgctca ggatagcgcg
     7981 ccacggcttg taaaaacgcc tgttcgccga agtcttctga aaactccagc gcggtcgggt
     8041 acgaggtgta tcgtggcccg gaatagttat atttctggat cagggccaga tcccagtcga
     8101 tttgctgtac agacatgctc actccttccg atggcgtctc tggcggctac ggcgtaccgg
     8161 cggatgcccg tttcgagtca tcagccggtt gcgcagccac ttttgtcgcc gcgacaaccg
     8221 ctgtagttta acgaataacc acaccagata acatataacc ggaagggtta tcagcagaac
     8281 gggaaggccc acggcggcaa tccgttagtt gccccgcagc agacgcatca tgtcttcttg
     8341 cttctcgtct tcttcctctt cttcgtcatc gtcataagag aggccgagtt tctgcatcaa
     8401 ctcatcaata cgatccagtt tggcatccac ccaggattgc tcttcggcgc tcagggtttc
     8461 gcctgcttcc agacgttcca gcagcgcatc cagacgctca tccgtttcca gtaactccaa
     8521 ctccgcctgc ggtgaaagca taggtttctc actcttcggt ttgtgctgct tggtgacttt
     8581 ttcagtcacg cccaatggaa taggggtttt actgccaata cgtggatctt ttggtgcgtt
     8641 ctgacctttg ctgcctgacg tggtgttacc gcccgctgcg cggctgcccg gcgcatgacc
     8701 acgacgtttt ttctgacgct tacggtcacg agcttcctga tccagctcct cgcgtgtttt
     8761 tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa gatgatggtt tcataatggt
     8821 tcgtctttta gccgttttat tcagtataga tttgcggcgg aatctaacag aaagcaagca
     8881 aagaaaaaag gcgacagatt actctgtcgc cttttttcct gactcataac ccttaacggg
     8941 tcttacaatc cttgcaggcc gccaacacac cctgtttttc cattagccgg aacaatccgt
     9001 gttgattcca tttccattta attacgtctc cggacgttta tcttcctgac aatcctctgt
     9061 cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc
     9121 tctatgggtg ctactttacg cctttgcttt aaataaacaa tcaaccgcca tcagtatttg
     9181 cacattttca gagttttata taaaagcaat tcactgattt atatagctta tttgacatac
     9241 gtcgcagaat tttctctgct atttcgcata aagactatcg gcaatgtctc acaaagcgta
     9301 tggcttttac ttacaacgct cacgccgaaa aatccgcctt ttattatggt tcaggtataa
     9361 tcgccgcaaa gctttgactg atggagacga ccgctttgac taatttgaat tatcaacaga
     9421 cgcattttgt gatgagtgcg cctgatattc gccacctacc ttccgatacc ggaattgaag
     9481 tggcttttgc aggccgttcc aacgcaggta aatccagcgc gctgaacacg ctgactaacc
     9541 agaaaagcct ggctcgtacc tcaaaaaccc cagggcgcac ccagcttatc aacctgtttg
     9601 aagtggctga cggcaagcgt ctggttgact tgcctgggta cggttatgcg gaagtcccgg
     9661 aagagatgaa gcgcaaatgg cagcgcgcgc tcggcgagta cctcgaaaaa cgtcagagcc
     9721 tgcaaggtct ggtggtgcta atggatattc gccatccgct gaaagatttg gatcagcaga
     9781 tgattgagtg ggcggtagac agcaatatcg ccgtgctggt actgctgacc aaagcggaca
     9841 aactggcaag cggcgcacgt aaagcgcaat tgaatatggt gcgtgaagcg gtgctggcgt
     9901 ttaacggtga tgtgcaggtt gaaacgtttt cttcgctgaa gaaacaaggc gtggacaagc
     9961 tgcggcagaa actggatacc tggtttagcg agatgcagcc tgtagaagaa acgcaggacg
    10021 gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg cgtatccggc ccgtaaattc
    10081 aatagattac aattctaccg ttggcctgat aaacatggcg tatcaggcat tacggatctt
    10141 ttctttcgcc caataaaaaa cgccccagtc attactgact ggggcggcta aaatattcag
    10201 ccaaatccga ttacgtgaag taaaaggtct gaaagataga acatcttacc tctgtaccct
    10261 acgcgactaa ctttactctt ttttgttcag ttcagaaagc attttttgta attttttttc
    10321 agttgttgca taggaatttc taatagccat cacaaaacgt gacagcttat gttgcttact
    10381 tacgaaaaaa gggcatgttc aggcaaatct tagtgcgcct gatcccagtt ttcgccactc
    10441 cccacttcca ccagcaacgg cacatccaga cgggtacagt tttccatcag ttgatgaatc
    10501 tgcttcgcga cggcatcgac atcatcttta tggacttcaa ataccagttc atcgtgtacc
    10561 tgcatgatca tacgaacgcg cggttgctca gcctgtagcc acgcatcaac ggcaatcatc
    10621 gcccgtttga taatgtcggc ggcggttccc tgcattggcg cgttaatggc tgcacgttca
    10681 gccgctgcac gacgagcacc attgctggat ttgatatccg gcagatacag acggcgtccg
    10741 tccagcgttt caacgtagcc ctgctctttc gcctgagcac gggtgcgttc catatactcc
    10801 agcacgccag ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc tttacgcgga
    10861 atgttcaatt gccgcgccag accgaaagca ctcatgccat aaatcagacc aaagttgatc
    10921 gctttcgcgc tacgacgttg ctcgctggtg acggtttcca gtggcaaacc aaacacttct
    10981 gccgccgttg cacggtggat atcttttcct tcggcgaatg cggtcagcaa gcctttgtca
    11041 cgcgagagat gcgccataat gcgcagttca atctgcgagt agtccgctga gacaatcaca
    11101 taatcctctg gcgcaataaa cgcctggcgg atacggcgac cttcttcgtt acgcaccgga
    11161 atgttttgca ggttaggatc ggttgacgat aaacgtcccg ttgcggttac tgcctggtga
    11221 taagaggtat gtacacgccc ggttttcggg ttgatcatca acggcagctt gtcggtgtag
    11281 gtcgatttca gcttcgccag accacgatac tccagaatca cttttggcaa cggatagtcc
    11341 agcgccagtt cttccagtac ctcttccgac gttgacggcg cgccacccgg agttttcttc
    11401 agcggtttaa tgccctgttt ttcaaacaga atggtttgta actgcttggt ggaagaaagg
    11461 ttaaattctt cacctgcaat ttcatgcgct ttcttttcca gctcagccag acgcaaggtg
    11521 agctcttcag agtgattgtg cagcactttc ggatcgatct tcacaccgtt acgttcaatg
    11581 cgtgaaagca ccggcaccag cggcatttcg atattctcga agacgttcaa cggccctttg
    11641 tgtttttgca gatccggcca cattttcaga tgcaactgca aggtgacatc tgcatcttcg
    11701 gcggcgtaac gtccggcttc ttcgagggca atctggttaa aggtcagttg atttttgcct
    11761 ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca accaacgttc cgcgaggctg
    11821 tccatatcgt gacgtccggc aacgctattg agaatgtagg actccaacat ggtatcaaac
    11881 gcaatcccac gtagttcaat gccgtagttc gccagaatac cgcgatcgta tttcaggttt
    11941 tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt ttagcagctc gagtgcacgc
    12001 tcgcgagaga tttgatcggg cgcatcaaga taatcatgag caaccggaat atatgccgct
    12061 acgcctggct cgatagcaaa agaaagcccg accaggttag cagagatgtt atcaaggctg
    12121 tcagtttcgg tatcaaatgc aaataccggc gctttttcca gcttcgcaat ccactctttc
    12181 agtgtttctt catcaaggat ggtgacgtag ttgtcataag aaatcaccgt tgccgtcact
    12241 tctggcgctt cgtctgcaac actggtttcc tgcggcctgg ctgcgggttt cgccccttta
    12301 gcctgtagcc attttcccgc ttcgacatca gcagtccagc gtttgaactc atactttttg
    12361 aacagcccta acaactcttc cgccgccggt ggctgcactt ccagttgttc acaggtcagc
    12421 tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg agagataagc aacttctttg
    12481 ttttgctcga gcttcgctgc cattgttttc gcgccacgga agctcaaccc agcaattttt
    12541 tctggctcgg catacagcgt atccagcccg ccaagacctt gcagcaatgc ctgcgcggtt
    12601 ttttcaccga cgcccggtac gccaggaatg ttatcagagg agtcacccat cagcgccagg
    12661 aaatcgatga tcagttctgg cggcacgccg tacttattca ccacctcttc cggtccgagg
    12721 atggtattcg tcatggtgtt gataagcgtg atatttggtg tcaccagctg cgccatatct
    12781 ttatcgccag tgctgatcag caccggacgc ccggcttttt cggcttcgcg cgccagagta
    12841 ccaataacgt cgtccgcttc tacgccagaa accgccagca gcggcagtcc catcgcttta
    12901 accatcgcgt gcagaggttc gatttgtgct cgcagatcgt ccggcattgg cgggcgatgt
    12961 gatttgtaat gttcaaacag ttcatcacga aaggtttttc ccttggcgtc aaagaccacc
    13021 gctgcatgcg tcggtttata ttgcatgatc agactgcgca gcatgttgag gacaccatac
    13081 atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg gaaacgcgtg atatgcgcga
    13141 taaaggtaag atgaaccatc tacaaggata agtggatttt gggggatctg aaccataatg
    13201 tccgtgcctg tttatcagat tatgggtaaa ggatgccaca gacaggatga aaacatgagt
    13261 ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg gatcgctcgc aaatcgtcat
    13321 gtggataact ttgtgcataa attatttaca tcgcttttaa tttattatca tgctttttac
    13381 agcatggtgt ttgtctttgt ttttcatgca gttaaaaata aagccatccc taataattga
    13441 gataatggga ttttttctaa tgtggataga tatgaattat ttttctcctt aaggatcatc
    13501 cgttatttgg gtcgttttct gctaaaatct gcgccttgcg cttctttagc gcactgtcta
    13561 tggctaacct tttgaataaa ttcattatga cgagaatact cgctgcgata acccttttgc
    13621 tgagtatcgt attgaccatt ttggtcacta ttttttgttc cgtcccgatc atcattgccg
    13681 ggattgtaaa actattgcta cctgtgccag tcatttggcg aaaggtttcg cgtttttgtg
    13741 attttatgat gtattgctgg tgtgaaggtc tggcggtatt actgcatctg aacccacacc
    13801 tgcaatggga agttcacggg ctggaagggt taagtaagaa gaactggtat ctgcttattt
    13861 gtaaccaccg tagttgggca gatattgtcg tactgtgcgt gctgtttcgt aagcacattc
    13921 cgatgaataa atatttcctc aagcagcagc tggcctgggt gccttttctt ggcctggcgt
    13981 gctgggcgct ggatatgccg tttatgaagc gttattcccg cgcttattta ttacgtcatc
    14041 cggagcgacg tggtaaagat gttgaaacca ctcggcgttc ttgtgaaaag tttcgcctgc
    14101 atcccaccac tattgtcaat ttcgttgaag gctcccgttt cacgcaagaa aaacatcagc
    14161 aaacccactc cacttttcaa aacttgttgc caccaaaggc ggcaggcatt gcaatggcgc
    14221 taaatgtact gggtaaacaa ttcgataaac tgcttaatgt tacgctgtgt tatccggaca
    14281 ataaccgtca gccgttcttc gatatgttaa gcggtaaact gacgcgaatt gtcgtacatg
    14341 tggatttaca gcctattgcc gatgagttac atggcgacta catcaatgac aaaagcttta
    14401 agcgccattt tcagcaatgg cttaactcat tgtggcagga aaaagaccgg cttctgacat
    14461 cgttgatgtc atcacagcgg caggaaaagt aagttttact cgcctctgaa tgagcagagg
    14521 cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac ataaattctt cttcgctcat
    14581 ctcctgaccg ttaaaaaaaa ctttacccgg tgtataacgc agttgcagcg aggcggtatt
    14641 gttgtcaatc gtgattaact gcaacatctg acctaatgtc atcatcccgc tgatttgttt
    14701 atcagcccgc ttttgcgctt tttccgcatc catcccttca gataaattaa gctgttttgc
    14761 ggtttctgtg gcgacattaa gcggtaactt catattaaaa ttgagcgatt taatatcttt
    14821 gtttgtggat gatgaagatt tggcggggtc ggcaatactg atatccagat tggcatttag
    14881 ttcgccgagt gcgttcttcc atcttactgg ttgtttaatg gtcggctcac ttttttgtaa
    14941 taacggtaaa tactctttga acaatgcagc attcacttct tgcagagcaa cttcatcgtt
    15001 tgctaactca gggtgtgcag caagctgctt ctgcatcgca atgttatact ggatgataaa
    15061 ttggcgcacc gctgagggat caatagactc agcaatcaat gaccactcac cactaccgag
    15121 ttgctgattc ccttttgtta acttatcaag ttcgtaggtc agattgacat tgatataatc
    15181 tttgtcttta acgcgatcca gcctggtttt tgcaacgaaa gcatcgattt gggcaacgac
    15241 ttccccctgg ttgataatat taacctgatt aaatttcgac tcgctttcac caaccggaaa
    15301 tttcttttct tctaatcgtg tcagagaatc aatggtaaaa cttttcgccg ttactttgtt
    15361 cattggtgaa aattgcagaa caatatcttc gactttgcct tcaatatcca ggttagacaa
    15421 tgaactatca tcaccattaa aagtaaactg gccttcacca aaagaaaact ttccatactc
    15481 tgcaggggca acatctaatg tcgtggagaa tgagccacca aacgcgaatc gcaatgtcgc
    15541 ttccacgggc gatttatttt tcgcggcgat aaatagcggt tgtgtcagct cattattcac
    15601 taagttcact tttgccgctg ccagtgctgg gatgacatta ccatgcatta acattgtgat
    15661 gggcaacgga ccgtgctcaa catccacatc aaatacaatt ttctggcctg gcttgatatt
    15721 gagatcgggt gcaccgttat caaatgtcat ttgcatttga aaacgggaat taaaaaagcc
    15781 tttgtcaaaa tttttataac ttaacatcat atcgtaggca tgttcacctt tctttttagc
    15841 atcgttaaaa cctttaataa acttttcgac accaggctga atttgcgtac ctgtgtacca
    15901 tgtgccacca ccccagatta cggctaacgc aacaatggca cctgtagct
//
LOCUS       DAZFRG010000108        15775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFRG010000108 DAZFRG010000000
VERSION     DAZFRG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.108"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..380)
                     /gene="acpS"
                     /locus_tag="ACHKUC_002702"
     CDS             complement(<1..380)
                     /gene="acpS"
                     /locus_tag="ACHKUC_002702"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6618938.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(380..1111)
                     /gene="pdxJ"
                     /locus_tag="ACHKUC_002703"
     CDS             complement(380..1111)
                     /gene="pdxJ"
                     /locus_tag="ACHKUC_002703"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO6618939.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1123..1851)
                     /gene="recO"
                     /locus_tag="ACHKUC_002704"
     CDS             complement(1123..1851)
                     /gene="recO"
                     /locus_tag="ACHKUC_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO6618940.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(1863..2768)
                     /gene="era"
                     /locus_tag="ACHKUC_002705"
     CDS             complement(1863..2768)
                     /gene="era"
                     /locus_tag="ACHKUC_002705"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO6618941.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(2765..3445)
                     /gene="rnc"
                     /locus_tag="ACHKUC_002706"
     CDS             complement(2765..3445)
                     /gene="rnc"
                     /locus_tag="ACHKUC_002706"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO6618942.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(3717..4691)
                     /gene="lepB"
                     /locus_tag="ACHKUC_002707"
     CDS             complement(3717..4691)
                     /gene="lepB"
                     /locus_tag="ACHKUC_002707"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO6618943.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(4707..6506)
                     /gene="lepA"
                     /locus_tag="ACHKUC_002708"
     CDS             complement(4707..6506)
                     /gene="lepA"
                     /locus_tag="ACHKUC_002708"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO6618944.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(6704..7183)
                     /gene="rseC"
                     /locus_tag="ACHKUC_002709"
     CDS             complement(6704..7183)
                     /gene="rseC"
                     /locus_tag="ACHKUC_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO6618945.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAIFGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7180..8136)
                     /gene="rseB"
                     /locus_tag="ACHKUC_002710"
     CDS             complement(7180..8136)
                     /gene="rseB"
                     /locus_tag="ACHKUC_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO6618946.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8136..8786)
                     /gene="rseA"
                     /locus_tag="ACHKUC_002711"
     CDS             complement(8136..8786)
                     /gene="rseA"
                     /locus_tag="ACHKUC_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO6618947.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(8819..9394)
                     /gene="rpoE"
                     /locus_tag="ACHKUC_002712"
     CDS             complement(8819..9394)
                     /gene="rpoE"
                     /locus_tag="ACHKUC_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO6618948.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9391..9546)
                     /gene="rseD"
                     /locus_tag="ACHKUC_002713"
     CDS             complement(9391..9546)
                     /gene="rseD"
                     /locus_tag="ACHKUC_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO6618949.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            9802..11424
                     /gene="nadB"
                     /locus_tag="ACHKUC_002714"
     CDS             9802..11424
                     /gene="nadB"
                     /locus_tag="ACHKUC_002714"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO6618950.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(11409..12146)
                     /gene="trmN"
                     /locus_tag="ACHKUC_002715"
     CDS             complement(11409..12146)
                     /gene="trmN"
                     /locus_tag="ACHKUC_002715"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO6618951.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12278..13612
                     /gene="srmB"
                     /locus_tag="ACHKUC_002716"
     CDS             12278..13612
                     /gene="srmB"
                     /locus_tag="ACHKUC_002716"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO6618952.1"
                     /translation="MTVTTFSELELDESLLESLQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(13645..14526)
                     /locus_tag="ACHKUC_002717"
     CDS             complement(13645..14526)
                     /locus_tag="ACHKUC_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6618953.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLALVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            14629..15216
                     /gene="eamB"
                     /locus_tag="ACHKUC_002718"
     CDS             14629..15216
                     /gene="eamB"
                     /locus_tag="ACHKUC_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO6618954.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15272..15655)
                     /gene="grcA"
                     /locus_tag="ACHKUC_002719"
     CDS             complement(15272..15655)
                     /gene="grcA"
                     /locus_tag="ACHKUC_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO6618955.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
BASE COUNT         3778 a         4232 c         3908 g         3857 t
ORIGIN      
        1 taactttcaa taattaccgt ggcacaagca tagtggcgct catcggcaag cgttacatgc
       61 atatttacaa cgccgagctt ttccgccagt tttaatgcct caccccatag ccgtagccgt
      121 ggtttgccga gctcatcatt gaatacttca aactgattaa acgccagacc attgcggatc
      181 cctgtgccaa atgcttttgc cgccgcttct ttcacagcaa aacgcttcgc cagaaaacgc
      241 actggctggt ggtgcgtttt ccagatttcc cattcgttat cgcttaatac gcggcgtgcc
      301 aggcgctcac cggatcgggc gatcaccgct tctatgcgag cgatctctac aatatccgta
      361 cctaaaccta atattgccat tagccacgcg cttccagcat cagacgtttc atttctgcca
      421 ccgcatcttt cagtccggtc atcactgcac gaccaataat ggcatgaccg atattcagtt
      481 catgcatctc agggatggcg gcaatggctt tcacgttgtg ataggtcaga ccgtgtccgg
      541 cgttaacttt cagaccgaga ctagtggcaa aggtcgcggc tttggcgata cgcgccaact
      601 cttgcgcctg ttcggcgtca gttttggcat cagcatagca accggtgtgg atctcgataa
      661 acggtgcgcc aacctctgcc gcagctttga tctgctcttc atcggcgtcg ataaacagag
      721 aaacctggat cccggcatct gccagacgtt tgcaggcatc gcgcattttt tcacgctgcc
      781 ctgcgacgtc caggccgcct tcggttgtta cttcctgacg cttttccggt accagacagc
      841 aaaaatgtgg cttcgtctca acggcgatcg ccagcatctc ttcggtcacc gccatctcca
      901 gattcatgcg ggtatccagc gtctggcgca ggatgcacac atcgcggtcg gtaatgtggc
      961 ggcggtcttc acgtaaatgc acggtaatgc cgtccgctcc cgcctgctca gcaataaacg
     1021 ccgcctgcac cggatccggg taagcagtac cgcgcgcgtt acgcagcgtt gcaatatggt
     1081 caatgttgac gcctaacagt aattcagcca tgacaatcct catcattcat aatgtgtttt
     1141 caccgttcgc ttaggcataa actgccggaa cagttccctg ctctttaatg gtttaccgcc
     1201 aagatacggc ttaagcgcca tgcgggtaaa gcgtttcgcg gcgcgcagtg tgtctgcgtc
     1261 aggaaattcc cgcgcgttta acgcttttaa ctgccttccg gtgaacgttt tattgtcgat
     1321 aacgacgctt gcgataaacc ctttttcttc gcgataacga tacgtcatgg tgtcatctac
     1381 cggctcgccg ctacccgcac aatgggtaaa attgacgcca tagcccagat gcccgagcag
     1441 tgccagttca aagcggcgca acgcgggttc tggcgtacca gtgaccccgg caagagactg
     1501 aatgcagtgc aagtaatcga aaaagagttc agagaagcgc gtctcgtatt ccagtacgcg
     1561 ggagagaagt tcgttgatgt acagaccgct gtaaagcgtg ataccgctta atggcagcgc
     1621 cagcgagaca gcttcagcac tgcgcagcgt tttgacttcg ccacgcccgc caaaacgtag
     1681 caagagaggg gtgaaaggct gtaatgcccc tttcagagta gagcgtttag agcgtgcgcc
     1741 tttggcaacc agacgcacgc gacccgattc ctccgtgaag acgtccagca tcaggctggt
     1801 ttcgctccac gggcgactat gcaggacaaa tgcgcgctgc cagccttcca tcggagttac
     1861 tcttaaagat cgtcaacata accgagactg cgcagtgcgc gttcgtcgtc ggcccaaccg
     1921 gatttcactt ttacccacag ctcaaggtga acaggcgctt cgaacatttc ctgcatgtct
     1981 ttacgcgctt caatcccgat ggttttgatc ttggcccctt tgttgccaat gaccatcttc
     2041 ttctgccctt cacgctcaac gagaatcaaa ccgttgatgt cataaccgcc gcgttcgtta
     2101 gagacgaaac gctcgatctc cacggtcacg gagtacggca gttcagcgcc gagaaaacgc
     2161 atcagttttt cgcggataat ttcagacgcc ataaaacgct gtgagcgatc ggtgatgtaa
     2221 tcttccggga agtgatgggt cgcttcaggc agatgcttac gcacgattgc cgcgatagtg
     2281 tcgacattca gcccggtttc ggcagagatc ggcacgatat cgaggaagtt catctggctt
     2341 gccaggaact gtaagtgtgg cagcagatcg gctttttcct gcacgttgtc cactttgttc
     2401 accgcgagga ttaccggcgc tttgccatcg cgcagtttgt tgagcaccat ttcatcgtcc
     2461 ggcgtccagc gggtgccttc tacgacaaaa atcaccagct caacgtcgcc gatagagctg
     2521 ctcgccgctt tgttcatcag gcggttaatg gcgcgttttt cttccatatg caggcctggg
     2581 gtatcaacgt agatcgcctg atacgcgcct tcagtatgga tccccacaat gcggtgacga
     2641 gttgtttgcg ccttgcggga ggtgatggag atcttctgcc ccagcagttt gttcaataat
     2701 gtggatttgc caacgttcgg acgtccgacg atagcaataa atccgcagta acttttatcg
     2761 atgctcattc cagctccagt tttttcaacg cctgttcggc ggcagcctgc tcagccttac
     2821 gacggcttga acctgtgcca accaccggtt cactcaggcc gctgacctgg cagtggatag
     2881 taaattcctg atcgtgcgct tcgccacgta cctggacgac cagataagtc ggcagcggca
     2941 gatggcgacc ctgcaaatat tcttgcaagc gcgttttcgg atctttttgt ttgtcacctg
     3001 ggctaatttc gtccagacgg gtttgatacc agttaaggat taatttctcg acggtttgaa
     3061 tatcactgtc gaggaatacg ccaccaatta atgcttcgac ggtgtcggcg agaattgact
     3121 cacgacgaaa tccaccgctt ttaagttcac ctggccctaa acgtaagcac tcgcctaact
     3181 caaattcgcg cgccagttct gccagcgtat tgccacggac cagcgtggcg cgcatccggc
     3241 tcatatcgcc ttcatccaca cgagggaaac ggtgataaag cgcattggcg ataacgtagc
     3301 tcagaataga gtcgcctaaa aattctaaac gctcgttatg tttgctgctg gcactacgat
     3361 gggttaatgc ctgctgcaac agttcctgat gattaaaagt gtagcccagc ttccgttgaa
     3421 gccgattaat tacgatgggg ttcatgcgat accaataaat aaatgcgtca acaattcagc
     3481 acacgaaaca gacctgatat acatggctcg gctaactgct tcactgcagt ttcagtttac
     3541 cggtatatgg ggaccaacgc tgtttcgtgt gccgtggcaa cctggaggtg ccaaccttaa
     3601 acttcggggg aatattctat acacaacgac gggggatgtc gttagccacg ggagatttat
     3661 ctcataaata attcacgttg tcgccataac ggcgacaacg tgaacgaaga tggctattaa
     3721 tggataccgc caatgcgact taagcgcaca ccagtcggcc attcgccttc ttgcttatcg
     3781 aagctcatcc agatagccgt tgcccgaccg accagattcg cttcaggcac aaagccccag
     3841 taacggctgt ccgcgctgtt gtcgcggttg tcgcccatca tgaagtattg tcccggcgga
     3901 acaatccagg ttgccagttg ttgccctggc tgctggtaat acatccccac ctggtcctgc
     3961 gcaatcggca ctgtcagaat gcggtgcgtc acatcaccca atgtctcttt acgctcggaa
     4021 agacgaattc cattttcttt ggtttcgttt ttcggcactt caaagaatcc gctggtcgct
     4081 tccccaccat tacggcgtga gaaggtctga acgaaatcgc tcggttccac gttggagtaa
     4141 gtgaccggta gcgcgttttc gcacgcctgg ccggaactgc atcccggttg aatcgtcagc
     4201 tcttttgaga ccggatcata agtgacttta tcgcccggca aacccaccgc gcgcttgatg
     4261 taatcaagct ttggatcttc cggatattta aagaccacga tatcgccgcg tttcggatga
     4321 ccggtttcga tcagcgtttt ctggtagata ggatctttaa tgccataagc aaacttctct
     4381 accagaataa aatcaccgat taacagagtc ggcatcatcg aacctgacgg gatctggaac
     4441 ggttcataaa taaacgaacg cacaatcaat acgatagcca gtaccggaaa aacagaagca
     4501 ccggtttcca gccagccagg cttcggcgca acctttttca gcgttgcttt atccagtgag
     4561 tcaccggcag ccgcctgcgc cgctgcctga cgttcccgcc gtttaggtgc gaaaaagaat
     4621 ttatccacgc accataaaat gcccgtcacc agtgtggcaa tcaccagaat cagggcaaac
     4681 atattcgcca tgccaactcc taagggttat ttgttgtctt tgccgacgtg cagaatggcg
     4741 aggaacgctt cctgcggcag ctcgacgtta ccgatctgct tcatgcgttt cttaccttct
     4801 ttctgcttct gcagcagctt tttcttacga ctgatatcgc cgccataaca tttagccagt
     4861 acgtttttac gcagctgttt cacggtggag cgcgcaatga tgtgcgtacc aatcgctgcc
     4921 tgaatggcga tatcaaactg ctggcgcggg atcagatctt tcatcttctc caccagctcg
     4981 cgaccgcggt tttgcgaatt accacggtgg gtgatcagcg ccaacgcatc aacacgttca
     5041 ccgttgatta atacgtctac acgtaccatg tcggacgcct ggaagcgttt gaagttgtaa
     5101 tccagagacg cataaccacg cgaggtagat ttcaggcgat cgaagaagtc gagcaccact
     5161 tccgccatcg ggatctcgta cgtcagcgcc acctgattac cgtggtaaac catattggtc
     5221 tgcacgccgc gtttttctac gcacagcgta ataacgttgc cgagatatgc ctgcggtagc
     5281 agcatgtgac actctgcaat cggctcgcgc agttcgtaaa tgttatttac cgctggcagc
     5341 ttggatgggc tgtcgacgta gataacttct cttgacgtgg tttcaacttc atacactaca
     5401 gtcggcgcag tggtgatcag atccagatcg tattcacgtt ccagacgttc ctggatgatc
     5461 tccatgtgca gcaggccaag gaagccgcag cggaaaccaa agcccaacgc gctggagctt
     5521 tccggctcat agaacagtga ggcatcgttc aggctgagtt tacccagcgc gtcacggaag
     5581 gcttcatagt cgtcggaact taccgggaac agaccagcat atacctgagg tttgactttc
     5641 ttaaagccag gcagcgcctt ttctgccgga ttacgcgcca gtgttaaggt atcgcctact
     5701 ggagcgccgt ggatatcttt aatcgcacat accagccagc ctacttcgcc acatttcagt
     5761 tcagtacggt caacctgctt cggcgtgaag atgcccagac gatcggcgtt ataggtctgc
     5821 ccggtgctca tgactttcac tttgtcgccc ttacgcaagg tgccgttttt aatacggata
     5881 agtgaaacaa cgcccagata gttgtcgaac caggagtcga taatgagtgc ctgcaacggg
     5941 ccttccggat cgccttccgg cggcggaatg tcgcgcacaa gacgttcgag aacgtcctgc
     6001 acgccaacgc cggttttcgc tgaacagcgc accgcgtcgg tggcgtcgat accgacgata
     6061 tcttcaattt cttccgccac gcgttcagga tcggctgccg gcaggtcaat cttgttcagt
     6121 accggcacca cttcgagatc catttccatg gcggtgtagc agtttgccag ggtttgcgct
     6181 tctacgccct gcccggcatc gaccaccagc agtgcgcctt cacaggcagc cagcgaacgg
     6241 gaaacctcat aggagaagtc tacgtggccc ggggtgtcga taaagttaag ctgataggtt
     6301 tcgccatcag acgctttgta gtccagcgtc acgctttgcg ctttgatggt aatgccacgc
     6361 tcacgctcaa gatccatgga atcgagaacc tgcgcctcca tttcacggtc agacaggcca
     6421 ccgcagatct ggataatacg gtcagacagc gtcgatttac cgtggtcaat gtgagctatg
     6481 atcgaaaagt tacgtatatt cttcataaag tatgattatt gtgccttaat gcccggttaa
     6541 ccaggctttt agaagtcgct gttctgagct taacgtctgt attaatagaa acgccgcatt
     6601 ctacactaca acattgaggc gaggaaatgt tcataccgta tggattgtgg tatctggaaa
     6661 cgtcctcgca tttgttatgc aaaatgcaac aaagccagtg aaatcactgg ctcgcgtctt
     6721 ccgaagatgt ttcaaatcgc acaaggccag gcggcaatgc cacgcttaag atgatcggtt
     6781 gccattcaga ccgggcagca aactttcgag agcagccacg tgcaatcagg aatccaccaa
     6841 cgccaccgaa aatcgcaccg cataatgctg cgacgtcaga agcaaagagt agctgaaata
     6901 gtgaagcgat gaggaatagt cccaccagcg gcgacatata aaccagtaat gcggagctaa
     6961 gcaggctgcc ttcggcgatt cctaattcca ctttttgccc cggcactaac ggctcatcac
     7021 agggtacgac aatggtatgc gtggtttgcg ggccaagttt attcaacacg cggctaccgc
     7081 aaccggcgcg tgaagcgcag ctgctgcatg aggctttaac atcgcaactg accagcgcct
     7141 gcccgttttc ccaggagacg acggtagccc actctttgat cattgcgctg ccccgaactt
     7201 aatattctcg gcaatgcgtt tcgccgtttg cggcggcagt tcaccgacaa tggtgatttc
     7261 ggcgttatca cgtacgcttg tactgacggt tctgcgcccg gtgcgcaaca tctgatcggt
     7321 gctcgatggc gtagcgcggt taacgtttac cgagaagctg aataatccgt cggaatagag
     7381 acgtgattcg ataggcatgt tgtccatcgt cggtagcgga cgtcgactac tggaaacttc
     7441 gctaaaaccc tgtggcagcc aggttggcgt ccagctgaat ttagcttttt caccgacagg
     7501 aacagaaagc aacggcggca aatttgcctt cgccagcgtc tgcatactgc tgccgatatc
     7561 ctgattgacg ttaaaagcaa tcacacgaaa ttgttccagc gtttcaccat cgcgatcaag
     7621 gagatcaacc cgcatcggta atttcgattc ggtgtccatc cacacgatgt agctgtagcg
     7681 tgtaccatct cgggcaacca cgcgaatgac ttcgcaaaga cgatcagcaa tacgcgtgcg
     7741 cccgacggag ataaagtcgt agtaaggaga aaggcgtttg aaatcggtat agatgagcga
     7801 tggcagagaa tcaacaatgt aatcgccatt aagcgtgaac ggttcaagtc ccggttcaaa
     7861 atagctgatt tcattgccgc gctgtaccac ttcccggcgc gggccatcca tttgcaacaa
     7921 ttgtgcaaga ggacggttat cgaggcgtgc atgtcgataa cgcagagact caacaccctg
     7981 tttattgatg ctgatgaatg acagctcgta attcagtgac tgactggcca ggttcatctg
     8041 ctgtaataac gccccggacg cgggagtggc cgaggcgtta gcagagaata acaggctacc
     8101 tgtcactaat gacatggcaa accaaagttg cttcattact gcgattgcgt tcctaaagtt
     8161 tgaattcctg gcacctgtac agcggcttgc tgagtttgtg cctgctcaaa ctgaagctgt
     8221 tcagagtgga gtcggcgttg cagttcgtaa tcctgcaaca ttgcattaat gcgacgacgc
     8281 tgctcctgta cctgctgctg ttgaccatta tttgcggtcg cttcagaagg tactcccaga
     8341 ctaaccgggc tggctttacc catcatcggc agtgtattaa atactggcgt ttcgggctgc
     8401 tgggacgttt cagattgccc attatagtgc tggacgccaa cgataactgc aagcgatacg
     8461 catgctgcta cgcccatttg ggtaagctgt gccgcccacg gacgtacttt ctgccagaat
     8521 ggcattttct gccattgatg cggcgcaggc tgggcttccg ggatcaatgt cgccgattga
     8581 cgtactggct cttcttcaat ggcggccatc acgcgtgaag agatatcgaa atggagcacc
     8641 tcgggagtat caccccgcat tgagtcacgg atcaagtgat agctttccca ggttttctgc
     8701 atttctgggt tatgagccag ttcgttaagc agctcactat ccagcgtttc gccatccatt
     8761 aaagcggaaa gttgttcttt ctgcatgcct aataccctta tccagtatcc cgctatcgtc
     8821 aacgcctgat aagcggttga actttgttat caatagcttc cctcgctcgg aagatacgtg
     8881 aacgcaccgt acctaccgga caatccatga tagcggctat ctcttcatag ctcaggccat
     8941 ccagctcccg caaggttatt gccatgcgta aatcttccgg gagggactca atagttcgga
     9001 aaactatctg tctcagttct tctgacaaca ttaagttctc agggttcgaa atttctttca
     9061 acgcgccgcc actttcgaag ttttcagctt caatggcatc cacatcactg gaaggtggac
     9121 gacgcccctg agcaaccagg taatttttcg ctgtatttac agcaatccga tacagccatg
     9181 tataaaaagc gctatctccc cggaacgaat ccagcgcacg ataggcttta ataaaagctt
     9241 cttgtaccac atcgggaaca tcacccgacg gcacatagcg ggaaaccaga ctcgccactt
     9301 tatgctgata gcgcactacc agtaagttaa aggctttctg atctcccttc tggacccgtt
     9361 caaccaggac ctggtccgtt aactgctcgc tcatccgagg taaagtctcc ccaaaccaaa
     9421 tttccacgcg ctatcgaaac gccactccat tagctgcaat ttgagcaagc aaagggttag
     9481 agtgtctcgt ttttgtaaag ttccgtaacg catctgtttt tgtttgtcat gctgtagacg
     9541 gatcattatc tatcattata agtctacaga atctgaacat cgcattatct gtgtagaaat
     9601 gcccatttaa ctgcctgaag agtaacccaa cggccttttt atttcaccac ctaatcctcc
     9661 accagccagt aacttctcgt tttctcgccg ccctgaggca gcgtgtttag tcactgtaac
     9721 aaatattaaa atagcaggtg tttattcgca caacatgatg ttatgctgac caaaccgtgt
     9781 ttagtaaatt aaacaaagaa aatgaatact ctccctgaac attcatgtga cgtgttgatt
     9841 atcggtagcg gcgcagccgg actttcactg gcgctgcgcc tggctgacca gcatcaggtc
     9901 atcgttctaa gtaaaggccc ggtaacggaa ggttcaacat tttatgccca gggcggtatt
     9961 gccgccgtgt ttgatgaaac tgacagcatt gactcgcatg tggaagacac attgattgcc
    10021 ggtgctggta tttgcgatcg ccatgcagtt gaatttgtcg ccagtaatgc acgatcctgt
    10081 gtgcaatggc taatcgacca gggggtgttg tttgataccc acattcagcc gaatggcgaa
    10141 gaaagctacc atctgactcg tgaaggtgga catagtcacc gtcgtattct tcatgccgcc
    10201 gacgccaccg gtagagaagt agaaaccacg ctggtgggca aggcgcagaa ccatccgaat
    10261 attcgcgtgc tggagcgcag caacgcggtt gatctgattg tttctgacaa aattggcctg
    10321 ccgggcacgc gacgggttgt tggcgcgtgg gtatggaacc gaaataaaga aacggttgaa
    10381 acctgccacg caaaagcagt ggtactggca accggcggtg cttcaaaagt ttatcagtac
    10441 accaccaatc cggatatttc ttctggcgat ggcattgcta tggcgtggcg cgcaggctgc
    10501 cggattgcca atctcgaatt taatcagttc caccctaccg cgctgtatca cccacaggca
    10561 cgcaatttcc tgttaacgga agcactgcgc ggcgaaggcg cttatctcaa gcgcccggat
    10621 ggcacgcgtt ttatgcccga ttttgatgag cgtggcgaac tggctccgcg cgatattgtc
    10681 gcccgcgcca ttgaccatga gatgaaacgc ctcggcgcgg attgtatgtt cctcgacatc
    10741 agccataagc ccgccgattt tattcgccag catttcccga tgatttatga aaaattgctc
    10801 gggctgggga tagatctcac gaaagaaccc gtgccgattg tgcctgctgc acattatacc
    10861 tgcggtggtg taatggttga tgatcatggg cgtacggacg tcgagggttt gtatgccatt
    10921 ggcgaagtga gttataccgg cttacacggc gctaaccgca tggcctcgaa ttcattgctg
    10981 gagtgtctgg tctatggctg gtcggcggca gaagatatca gcagacgtat accttatgcc
    11041 cacggcgtca gtacgttacc gccgtgggat gaaagccgcg ttgagaaccc tgacgaacgg
    11101 gtagtaattc agcataactg gcacgagcta cgtctgttta tgtgggatta cgttggcatt
    11161 gtgcgcacaa cgaagcgcct ggaacgcgcc ctgcggcgga taaccatgct ccaacaagaa
    11221 atagacgaat attacgccca tttccgcgtc tcaaataatt tgctggagct gcgtaatctg
    11281 gtacaggttg ccgagttgat tgttcgctgt gcgatgatgc gcaaggagag ccgtggcctg
    11341 catttcacgc tggattatcc ggaactgctc acgcattccg gtccatcgat cctttctgcc
    11401 ggcaatcatt acataaacag ataaaaagcc tgggtcagcg ccgtatacgc ttcggaatag
    11461 ttctggtctg gcccacgaat gactaagcga tcgctaaagc attctcccgc ctgcggggag
    11521 aatgccagca gcacccgatg cggcagtcgc gcttcgtttt ccgccacatc cgtccgcaaa
    11581 cgtaaatgcc agcccatact taatgccagt tcagtaaaac cattaccaat ctgctctggc
    11641 agcaccacgc agaaaaatcc ctcttcggta atgcactccg ccgcacaagt cagcaacgat
    11701 gggtgatcaa gcgtagtggt atagcgagcc tgttcccgtt gaggtgtcgc gcactctact
    11761 ccctgctgat agtaaggtgg gttgctgatg attaaatcga agcgtactgt ctgctgtgtg
    11821 atccactgca gaatatccgc cgtatggacg ttaatccgct ctgcccacgg ggactggttg
    11881 atattttcct gcgcttgcgc ggcagcttca ctttccagtt caactgcatc aatcatcacg
    11941 ctgtcatctg ttcgctgcgc cagcattaat gccaacaacc cgctacccgc gccgatatca
    12001 aggcaacgtt ttaccccagc cacaggtgcc catgcgccca gcaaaatgcc atccgttccc
    12061 actttcatcg cacagcgatc gtgagcaaca aaaaactgtt taaaagtaaa tccattacga
    12121 cgaaacacgg atgtagactg tgacatgaaa ataaaacctt gcaggaaaaa cggcgatagc
    12181 accgggtgag aacaataccc gagaagcgat atccatacaa acagatgaag attgcagccg
    12241 taacgtctat aatcagcgcc ccacacagag gtagaacatg actgtaacga ctttttccga
    12301 acttgaactc gacgaaagcc tgctggaatc cctccaggat aaaggtttca ctcgcccgac
    12361 cgccattcag gctgccgcca ttccgcctgc gctcgatggc cgtgatgtac tcgggtctgc
    12421 gccgacaggc accggtaaaa cggcggcgta tctgctgcca gcgttgcagc acctgctcga
    12481 tttcccacgt aagaaatccg gtccgccgcg tattttgatc cttaccccga ctcgcgaact
    12541 ggcgatgcag gtggccgatc atgcccgcga actggcgaaa catacgcatc tggatattgc
    12601 caccatcacc ggcggcgtgg cctatatgaa ccacgcagaa gtgttcagcg aaaaccagga
    12661 cattgtggtc gccacgaccg gacgtctgct gcaatacata aaagaagaga acttcgattg
    12721 ccgcgcggtt gaaacgctga tcctcgacga agcagaccgt atgctggata tgggcttcgc
    12781 ccaggatatc gaacatattg ctggcgaaac gcgctggcgt aaacagaccc tgctcttttc
    12841 ggcaactctg gaaggcgatg cgattcagga ctttgccgag cgtctgctgg aagatccggt
    12901 ggaggtttct gccaatccgt ccacccgtga gcgcaaaaaa attcatcagt ggtattaccg
    12961 cgccgatgat cttgagcata aaaccgcgtt gctggtgcat ctgttaaaac agccggaagc
    13021 gacccgctca attgtgtttg tgcgtaagcg tgagcgtgtg catgagctgg caaactggct
    13081 gcgcgaagca ggcatcaaca actgctatct cgaaggtgag atggtacaag gcaagcgtaa
    13141 cgaagcgatt aaacgcttga ccgaaggccg cgtaaacgta ctggttgcta ccgatgttgc
    13201 cgcgcgcggt atcgacattc ctgacgtcag ccacgtcttt aacttcgata tgccgcgcag
    13261 tggcgatact tatttgcacc gtatcggacg taccgcgcgc gccggtcgta aaggcaccgc
    13321 gatttcgctg gtggaagccc atgaccatct gctgctgggc aaagttggtc gctatattga
    13381 agagccaatt aaagctcgcg taattgacga gttacgcccg aaaacgcgtg cgccaagcga
    13441 gaagcagacc ggcaagccat cgaagaaagt actggcaaaa cgcgccgaga agaaaaaagc
    13501 taaagagaaa gagaagccgc gggtgaaaaa acgccatcgc gacaccaaaa atattggtaa
    13561 gcgccgtaaa ccaagcggaa cgggcgtgcc accgcaaacg acagaagagt aatctcaatg
    13621 ccgggtttaa gcccggcatt gtttttatcc cggcaaaaaa cactcttcaa cacactgaat
    13681 aaaagtgtgc atcgccgggc taaccgcttt tccggcatgg tgagcgcaca ttgccgtaat
    13741 ggtctgcgac tgttcgccaa aaggcaattc aattaattct ccgcactcca actcttttac
    13801 caccgcaaaa cgcggcaggt aactgactcc gatatttgcc gcgacacaac gcttgatgct
    13861 ttcgatactt ataagctcaa tggtgttttc caccgtgatc cgccgctggc gcagcgtgct
    13921 ctcaaatatc tgccggaaga cacattgtgg ttcgttgata ataaagctac aggcgttatg
    13981 tcttcccggc tcagtaaaat cgacatctgc aatttgcggt gaagccacca gcgccagcgg
    14041 ttgttcaccc aactctcgtc ggttcagagc atcatcattc cctacgcgat aaaaaacccc
    14101 gacatcggcc tcatcattca gtagcgcatc acggatcacg taacagttca gcgactgcaa
    14161 cgataaacgc acttttggag cgcgttgccg aaaacgctgc aacacctgcg gcattcgata
    14221 agagagcaac gtttcgcctg aaataacgcg tagttccccg tccggatccg actctttttt
    14281 agcagcctca cgaagcgtat ccatcactcg ggtaagttca tagatgtgcg gcagcagctt
    14341 ttttccttcg cgggtaaggc acattcgtcg accaattttc tcaaacaatt gtaccgagaa
    14401 ctcctgctca agctgctgaa tatgaaaagt caccgtcgat tgtgtgcagc acaatttttg
    14461 cgaagctcgc aaaaaggaac cctcttccac cacggtttta agtgtaataa aacggcgcag
    14521 atccataacc ccaaacctat cgaaaatatc gaatttagaa tgtaaaaaca ttcatttttt
    14581 taaatgttcc gtgtcgggta ctgtctacca aaacagagga gataacaagt gacaccgacc
    14641 cttttaagtg ctttttggac ttacaccctg attaccgcca tgacgccagg accgaacaat
    14701 attctcgccc ttagctctgc tacgtcgcat ggatttcgtc aaagtacccg tgtgctggca
    14761 gggatgagtc tgggattttt gattgtgatg ttactgtgtg cgggcatttc attttcactg
    14821 gcagtgattg acccggcagc ggtacacctt ttgagttggg cgggggcggc atatattgtc
    14881 tggctggcgt ggaaaatcgc caccagccca acaaaggaag acggacttca gacaaaacca
    14941 atcagttttt gggccagctt tgctttgcag tttgtgaacg tcaaaatcat tttgtacggt
    15001 gttacggcac tgtcgacgtt tgttctgccg caaacacagg cgctaagctg ggtagttggc
    15061 gtcagcgttt tgctggcgat gattgggacg tttggcaatg tgtgctgggc gctggcgggg
    15121 catctgtttc agcaattgtt tcgccagtat ggtcgccagt taaatatcgt gcttgccctg
    15181 ttgctggtct attgcgcggt acgcattttc tattaacgaa aaaaagcgga agaggtcgcc
    15241 ctcttccgct tagtaacttg ctacttaagc cttacaggct ttcagtaaag gtacgagcga
    15301 taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg aactgcataa ccggatacac
    15361 ggatggtcag ctgcggatat ttttccggat gcttaactgc atcttccaga gtttcacgac
    15421 gcagaacgtt tacgttcagg tgttgaccac cttcaacgcg aacttctggt ttcacttcta
    15481 ctggaacttc acggtactca atgtcaccca gtttgcttac tgcaaccact tcatcttctg
    15541 cataacctgc ttttgcaacg atgcaacgcg cttcgccttt ttcgctgtcc agcagccaga
    15601 aagagttcag cagatcgtcg ttagcggctt tagtaatctg gatacctgta atcatgtgat
    15661 gcctccccgg caaaattatt tgatttgttc agcctgtcgc ggccaattgg taaaaccatt
    15721 gttgcttgag tgtatatata ctcctcaaac acccttgaat ctttgattta aatca
//
LOCUS       DAZFRG010000109        15406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFRG010000109 DAZFRG010000000
VERSION     DAZFRG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.109"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..572)
                     /locus_tag="ACHKUC_002720"
     CDS             complement(<1..572)
                     /locus_tag="ACHKUC_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO6618956.1"
                     /translation="MTESLDYIDLNKLELDTKNPRLPEGVERTPEAMLNHIALTTSIE
                     DLMNAIAENGFFPGEPLIAVKEGDKYTVVEGNRRLTAVKLIHNPYECDRPSSRMIEIA
                     ESAKDKLGTLEKLPVIVRDTRAEILPYLGFRHITGVKQWEPLSKARYIEQLFGLTSPN
                     SPTNDRYHQVARAIGSRKDHIKRNLDALAV"
     gene            complement(948..1982)
                     /locus_tag="ACHKUC_002721"
     CDS             complement(948..1982)
                     /locus_tag="ACHKUC_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6618957.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWIGQEVIRF
                     KNYSLDTDNG"
     gene            complement(1982..3754)
                     /locus_tag="ACHKUC_002722"
     CDS             complement(1982..3754)
                     /locus_tag="ACHKUC_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO6618958.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            3928..4782
                     /locus_tag="ACHKUC_002723"
     CDS             3928..4782
                     /locus_tag="ACHKUC_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6618959.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            4841..5914
                     /locus_tag="ACHKUC_002724"
     CDS             4841..5914
                     /locus_tag="ACHKUC_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6618960.1"
                     /translation="MRPETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATEEPGA"
     gene            5918..6661
                     /locus_tag="ACHKUC_002725"
     CDS             5918..6661
                     /locus_tag="ACHKUC_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO6618961.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVNPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAVTRAPRKTRSVTPAKRGRPKKKAS"
     gene            6761..7270
                     /locus_tag="ACHKUC_002726"
     CDS             6761..7270
                     /locus_tag="ACHKUC_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6618962.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTCLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            7270..7473
                     /locus_tag="ACHKUC_002727"
     CDS             7270..7473
                     /locus_tag="ACHKUC_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6618963.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGVVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            7477..7758
                     /locus_tag="ACHKUC_002728"
     CDS             7477..7758
                     /locus_tag="ACHKUC_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6618964.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            7758..8255
                     /locus_tag="ACHKUC_002729"
     CDS             7758..8255
                     /locus_tag="ACHKUC_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO6618965.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            8270..8695
                     /locus_tag="ACHKUC_002730"
     CDS             8270..8695
                     /locus_tag="ACHKUC_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO6618966.1"
                     /translation="MKKLSLSLMLNVSLALMPALSLIYPQSVAVSFVATWAILAMVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLICTACRQRLEEREPCQS"
     gene            8683..9108
                     /gene="lysB"
                     /locus_tag="ACHKUC_002731"
     CDS             8683..9108
                     /gene="lysB"
                     /locus_tag="ACHKUC_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO6618967.1"
                     /translation="MSKLMIVLVVLLSLAVSGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            9008..9253
                     /gene="lysC"
                     /locus_tag="ACHKUC_002732"
     CDS             9008..9253
                     /gene="lysC"
                     /locus_tag="ACHKUC_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO6618968.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            9216..9683
                     /locus_tag="ACHKUC_002733"
     CDS             9216..9683
                     /locus_tag="ACHKUC_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618969.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            9676..10128
                     /locus_tag="ACHKUC_002734"
     CDS             9676..10128
                     /locus_tag="ACHKUC_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6618970.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            10195..10830
                     /locus_tag="ACHKUC_002735"
     CDS             10195..10830
                     /locus_tag="ACHKUC_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6618971.1"
                     /translation="MNTLANIQELARALRNMIRPGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            10827..11174
                     /locus_tag="ACHKUC_002736"
     CDS             10827..11174
                     /locus_tag="ACHKUC_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6618972.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            11179..12087
                     /locus_tag="ACHKUC_002737"
     CDS             11179..12087
                     /locus_tag="ACHKUC_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO6618973.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            12080..12610
                     /locus_tag="ACHKUC_002738"
     CDS             12080..12610
                     /locus_tag="ACHKUC_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6618974.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTVGAIRRVVEPLGYLINVKE
                     WWETNDPPGTFRLDIGVLESGITEEMYLEMERLIADAKPASRHLIGLNIIQDIPGYLY
                     TGGVVCDGDVITVYPG"
     gene            12621..15362
                     /locus_tag="ACHKUC_002739"
     CDS             12621..15362
                     /locus_tag="ACHKUC_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6618975.1"
                     /translation="MSTKFKTVITTAGAAKLAAATVPGGKKVNLSAMAVGDGNGKLPV
                     PDAGQTKLVHEVWRHALNKVSVDNKNKNYIVAELVVPPEVGGFWMRELGLYDDAGTLI
                     AVSNMAESYKPELAEGSGRAQTCRMVIILSNVASVELSIDASTVMATQDYVDDKIAEH
                     EQSRRHPDATLTEKGFTQLSSATNSTSEKLAATPKAVKAANDNANSRLAKNQNGADIQ
                     DKSAFLDNIGVTSLTFMKHNGMIPTTDNLDSYGPEEKYLGTWSCPSQSTAKPESGYPE
                     DKGNGVLEVFNAGRFHCTQRYTTRTGNIYIRMLDAEWNPASPTWSAWRVITSGTRPLS
                     TSIDLNSLGGAEHLGIWRNSSTSIASFERHFPEDGSFGQGILEVFEGGLYGRMQRYTT
                     RSGTMYIRGLTASWDAENPQWEDWIAVGYQSTGWTYSGDLDDLLKPGIYSVTKQATNA
                     PVTDSKDLAVGSIVEVKKRCDIESYIQTYTTVSATDAYKNRTFQRTRASGEADWGEWA
                     EVYNSKSLLTKLGVGGVTDRLSSLDWQTYDFVPGSMITVRLSDMTNIPDGMEWGVIDT
                     NLINITVGPSEGGGVARSMQVWRSTSNKTNYRFFTVRLYGNPGERSFNIRRLPIIDEA
                     QTWEAKQTFSAGLSGELSGNAATATKLKTARKINNVSFDGTSDINLTPKNIGAFASGK
                     TGDTVANDKAVGWNWSSGAYNATTGGASTLILHFNIGEGSCPAAQFRVNYKNGGIFYR
                     SARDGYGFEADWSEFYTTTRKPTAGDVGALSLSGGQLNGALGIGTSSALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANSVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDV
                     RLGTRVVQTMQKGVMYEKSGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
BASE COUNT         3581 a         4011 c         4394 g         3420 t
ORIGIN      
        1 taaacggcta aagcatctag atttctcttg atatgatctt ttctgctacc gattgcacga
       61 gcaacctgat gatatcgatc attagtcggt gagtttgggg aggtgagacc aaaaagttgc
      121 tcgatataac gagccttcga tagaggttcc cattgtttga caccagtgat atgtctgaaa
      181 cccaaataag gtaaaatttc agctctggta tctctaacga tcactggtaa tttttctagt
      241 gtacctagtt tatctttagc gctttcggcg atttctatca tccgggagct gggtctgtcg
      301 cactcatatg ggttatgaat aagtttaact gccgttaagc gacggttgcc ttccacaacg
      361 gtgtacttat ctccctcttt aaccgcaata agaggctcgc caggaaaaaa tccattttct
      421 gcgattgcat tcatcaagtc ttcaatagag gttgttagag ctatatgatt tagcattgct
      481 tctggggtac gttcaacacc ttcaggaaga cgtggatttt ttgtatcaag ctcaagttta
      541 ttgaggtcga tgtaatcaag gctttctgtc ataactctaa tctcattaga tatggtgata
      601 tgcaaagatt aacactggag ttaaacgtag gctagaactg caattatgtg cattaaaatg
      661 catgcgtaag taactcgcta aaatgaagcc ataaactcac agtggtgcta tgaataatat
      721 gttatgcaac tgcattaaaa ccgacccatg aagcgggcgg gcgaggcggg gaaagcactg
      781 cgcgctggcg gtggtgcgga ttttattttt tcagcgtctg agcgtgtcgt gacggcgttt
      841 agattgtgcg ccggggcatt ggtgtgtctg cggggtgttt tgtgcggtgg tgagcgtgtg
      901 agggcgtgat gacgggatat aaaaaagccg cccgcaggcg gcgatgttca gccgttgtca
      961 gtgtccagtg agtagttttt aaagcggatg acctcctgac cgatccagcc gtttatctcg
     1021 cggatcctat cctgtaacgg gataagctca ttgcggacaa agacctttgc cactttctca
     1081 atatcgccca gtgacccgac gttctccggc ttgccgccca tcaactgaaa ggggatgcgg
     1141 tgcgcgtcca gcaggtcagc ggcgctggct tttttgatat taaaaaaatc gtccttcgtt
     1201 gccacttcac tgagcgggat aattttgatg ccgtcggctt tcccctgtgg ggcatagaga
     1261 aacagatttt taaagttgtt gcggcctttc gacttcacca tgttttcgcg gagcatttcg
     1321 atatcgttgc gatcctgcac ggcatcagtg acgtacatga tatatccggc atgagcgccg
     1381 ttttcgtaat acttgcggcg gaacagcgtg gccgactcat tcagccaggc agagttaagg
     1441 gcgctgagat attccggcag gccgtacagc tcctgattaa tatccggctc cagcagatga
     1501 aacacggagc cgggcgcgaa ggctgtcggc tcgttgaagg acggcaccca ccagtaaaca
     1561 tcctcttcca cgccacgacg ggtatatttc gccggtgagg tttccagtct gatgacctta
     1621 ccggtggtgc tgtagcgctt ttccagaaac gcattaccga acaccagaaa atccagcaca
     1681 aagcggctga aatcctgctg ggaaagccac gggtgcggga taaacgttga agccagaata
     1741 ttacgtttga cgtaaatcgg tgagctgtga tgcacggcag cacgcagact ttttgccaga
     1801 ccggtaaagc tgaccggcgg ctcataccat ctgccgttac tgatgcattc gacgtaatcc
     1861 agaatgtcac ggcggtcgag taccggcacc ggctcgccaa aggtgaatgc ctccattttc
     1921 ggggcgctgg cggtcattgt ttttgccgca ggttgcggtg ttttcccttt tttcttgctc
     1981 atcagtaaaa ctccagaatg gtggatgtca gcggggtgct gataccggcg gtgagtggct
     2041 catttaacag ggcgtgcatg gtcgcccagg cgaggtcggc gtggctggct tcctcgctgc
     2101 ggctggcctc ataggtggcg ctgcgtccgc tgctggtcat ggtcttgcgg atagccataa
     2161 acgagctggt gatgtcggtg gcgctgacgt catattccag acagccacgg cggataacgt
     2221 cttttgcctt gagcaccatt gcggttttca tttccggtgt gtagcggata tcgcgcgcgg
     2281 caggatagaa cgagcgcacg agctggaaca cgccgacacc gaggccggtg gcatcaatac
     2341 cgatgtattc gacgttatat ttttcggtga gtttgcggat ggattcagcc tgggtggcaa
     2401 agtccatgcc tttccactgg tgacgctcaa gtattctgaa tttgccaccg gccaccaccg
     2461 gcggtgccag taccacgcat ccggcactgt cgccacggtg tgacgggtcg taaccaatcc
     2521 ataccgggcg ggagccgaac ggattggcgg caaagggtgc atagtcttcc cattcttcca
     2581 gcgtgtcgac catgcagcgt tgcagctcct cgaacgggaa caccgatgcc ttgtcgtcaa
     2641 caaattcaca catgaacagg tttttaaaat cgtcggcgct gttttcgcgt ttgagctgct
     2701 caatgtcgaa cagcgtgcag ccgcctttca gggcgtcctc aatggtgaca atctgccgcc
     2761 actggccgtc cgcacagaga agacctccgg caagggcgtt atgactgacg tcgatttcca
     2821 cgcgttcggc ggcgctggcg cgtccccggt tgaacagttc acccgaccag aacgggtagg
     2881 cgtcgtgcgc cagcgtggac ggggtggaga aataggtcga gcgcaggtgg ctctgtgagg
     2941 ccatacctga tgccacctta cgcagtacct gaaaattcgg gatccagaaa atctcgtcga
     3001 cgtacaggtc gccgttatgg ctctgtgcgg tgttggagtt ggtgccgaga aaaatcagtt
     3061 ttgcgccgtt attgcccagg acaatcgggt caccggtcag gtcaacgtca accagacggg
     3121 caaaggcgat gatgtattcg cggaacacat acgcctgcgt tttactggcc gacagaaaaa
     3181 tctggttatg accggttttc agggcacgca gcagcgcctc gcgggaaaaa taaaacgtcg
     3241 cgccaatctg gcgggatttc aggatatctc ggatgcggtg ctcaagcccg gcgcgatacc
     3301 agtgcaactg atattcgaaa gactgctcaa agaaaatctg ctccagcttt tcgatggcct
     3361 cgtcactgaa aaaattcttt ttcggtttgc gacgcccgcc tttgttgcgg ttagcgacgt
     3421 tcggattaag gtctgcctcg ttgccggtct gactgtagcg gttgacccgt gccagtcgtt
     3481 caatctggcg tcccagcagg tcaatttcct tgaagtcacc gccggttttc tgcggtttga
     3541 tgatgagctg ggtcagccgc gcttccagac tcatttcgac acggctgatg ggggcaacgc
     3601 tgtcccagcc gtcgcgctgt ttccagctct gcaccgtcgg gcgtttcatc tgcaacatgg
     3661 cggcaatctg cggcacggaa aacccctgcc agtacagcag cgccgcctga cgacgcgggt
     3721 cgtgtaaaag agtggtgtct gtggtgatgg tcatgaatac ctcgccgtga tgaatacacg
     3781 gcaaggctac tgagtcgcgc cccgcgattc gctaaggtgc tgttgtgtca gtgataagcc
     3841 atccgggact gatggcggag gatgcgcatc gtcgggaaac tgatgccgac atgtgactcc
     3901 tctaatcact attcaggact cctgacaatg gcaaaaaaag tctcaaaatt ctttcgtatc
     3961 ggcgttgagg gtgacacctg tgacgggcgt gtcatcagtg cgcaggatat tcaggaaatg
     4021 gccgaaacct ttgacccgcg tgtctatggt tgccgcatta acctggaaca tctgcgcggc
     4081 atcctgcctg acggtatttt taagcgttat ggcgatgtgg ccgaactgaa ggccgaaaag
     4141 attgacgatg attcggcgct gaaaggcaaa tgggcgctgt ttgcgaaaat caccccgacc
     4201 gatgacctta tcgcgatgaa caaggccgcg cagaaggtct acacctcaat ggaaattcag
     4261 ccgaactttg ccaacaccgg caaatgttat ctggtggggc tggccgtcac cgatgacccg
     4321 gcaagcctcg gcacggaata cctggaattc tgccgcacgg caaaacacaa ccccctgaac
     4381 cgcttcaaat taagccctga aaacctgatt tcagtggcaa cgcccgttga gctggaattt
     4441 gaagacctgc ctgaaaccgt gttcaccgcc ctgaccgaaa aggtgaaatc catttttggc
     4501 cgcaaacagg ccagcgatga cgcccgtctg aatgacgtgc atgaagcggt gaccgctgtt
     4561 gctgaacatg tgcaggaaaa actgagcgcc actgagcagc gcctcgctga gatggaaacc
     4621 gccttttccg cacttaagca ggaggtgact gacagggcgg atgaaaccag ccaggcattc
     4681 acccgcctga aaaacagcct cgaccacacc gaaagtctga cccagcagcg ccgcagcaag
     4741 gccaccggcg gtggcggtga cgccctgatg acgaactgct gaccggcgtc agtcagtccg
     4801 gaaaaacctt cacgattaac ccttaatttc aggaaaaact atgcgcccgg aaacccgctt
     4861 taaatttaat gcctacctgt cccgtgttgc cgaactgaac ggcatcgacg ccggtgatgt
     4921 gtcgaaaaaa ttcaccgttg aaccgtcggt cacccagacc ctgatgaaca ccatgcagga
     4981 gtcctctgat ttcctgaccc gcatcaacat tgtgccggtc agcgaaatga aaggggaaaa
     5041 aattggcatc ggtgtcaccg gctccatcgc cagcaccacc gacaccgccg gtggcaccga
     5101 gcgtcagccg aaggacttct cgaagctggc gtcaaacaag tacgaatgcg accagattaa
     5161 cttcgatttt tatatccgct acaaaacgct tgacctgtgg gcgcgttatc aggatttcca
     5221 gctccgtgtc cgtaacgcca ttatcaaacg ccagtccctt gatttcatca tggccggttt
     5281 taacggcgtg aagcgtgccg aaacctctga ccgcagcagc aatccgatgc tgcaggatgt
     5341 ggcggtcggc tggctgcaga aataccgcaa tgaagccccg gcgcgcgtga tgagcaaggt
     5401 cactgacgag gaagggcaca ccacctctga ggtcatccgc gtgggtaagg gcggtgatta
     5461 tgccagcctc gatgcactgg tgatggatgc gaccaacaac ctgattgagc cgtggtatca
     5521 ggaagaccct gaccttgtgg tgattgtggg gcgtcagctg ctggcggaca aatatttccc
     5581 catcgtcaac aaggagcagg acaacagcga aatgctggcc gctgacgtca tcatcagcca
     5641 gaaacgcatc ggtaacctgc cggcggtacg cgtcccgtac ttcccggcgg atgcgatgct
     5701 catcacgaag ctggaaaacc tgtccatcta ctacatggat gacagccatc gccgcgtgat
     5761 tgaggaaaac ccgaaactcg accgcgtgga gaactacgag tcaatgaaca ttgattacgt
     5821 ggtggaagac tacgccgccg gttgtctggt ggaaaaaatt aaggtcggtg atttctccac
     5881 accggctaag gcgaccgaag agccgggagc gtaaccgatg acgagtcccg cacagcgcca
     5941 catgatgcgg gtctcggcag cgatgaccgc gcagcgggaa gccgccccgc tgcgacatgc
     6001 aactgtctat gagcagatgc tggttaagct cgccgcagac cagcgcacac tgaaagcgat
     6061 ttattcaaaa gagcttaagg ccgcgaaaaa acgcgaactg ctgccgttct ggttgccgtg
     6121 ggtgaacggc gtgctggagc agggcaaagg tgcacaggat gacattctga tgacggtcat
     6181 gctgtggcgt ctggataccg gcgatattgc cggtgcgctg gagattgccc gttatgccct
     6241 gaagtacggt ctgaccatgc cgggtaaaca ccgccgcacc ccgccgtaca tgttcaccga
     6301 ggaggtggcg cttgcggcca tgcgcgctca cgctgccggt gagtctgtga atccccgcct
     6361 gctgacggac acccttgaac tgaccgccac ggctgacatg cctgatgaag tgcgcgcaaa
     6421 gctgcacaaa atcaccggtc tgtttctgcg tgacgctggt gatgccgcag gggcgctggc
     6481 gcacctgcaa cgtgcgacac agctcgactg tcaggcaggc gtcaaaaaag agattgaacg
     6541 actggagcgg gagctgaaac cgaagccgga gccgcagcca aaagcggtca cccgcgcccc
     6601 gcgtaagacc cggagcgtga caccggcaaa acgtggacgc ccgaaaaaga aagccagtta
     6661 acaaccgaat gcgccccgcg ccagggcggc acgccggtca gtgagggtga atcacctgac
     6721 actgcaccgg cgtccaccgc ccgacttttc agaggtagtc atgatgacgc tgattattcc
     6781 gcgaaaggag gctcccgtgt ccggtgaggg gacggtggtc atcccgcaac cggcaggcga
     6841 cgagccggtg attaaaaaca cgttcttttt tcccgatatc gacccgaagc gcgtccggga
     6901 acgtatgcgc cttgagcaga ccgtcgcccc cgcccgtctg cgtgaggcca tcaagtcagg
     6961 catggcggag acgaatgcgg agctgtacga ataccgcgaa cagaaaattg ccgccggttt
     7021 tacgtgtctg gcggacgttc cggcggacga catcgacggt gaaagcatca aagtttttta
     7081 ctacgagcgc gccgtgtgtg cgatggcgac cgcatcgctt tatgagcgtt atcgcggcgt
     7141 ggatgccagt gcgaaaggcg acaagaaggc tgacagcatt gacagcacca ttgatgagct
     7201 gtggcgggat atgcgctggg cggtggcgcg catccaggac aagccgcgct gcatcgtgag
     7261 tcaaatctga tgaagacctt tgcgctacag ggcgacacgc tcgacgccat ttgtgtccgg
     7321 tattacgggc gcactgaggg cgtggttgag accgtgctcg ccgcaaatcc gggactggct
     7381 gaactgggtg tggtgctgcc acacggcacc gccgtcgaac tgcccgatgt tcagaccgcg
     7441 cccgtggctg aaactgtcaa tctgtgggag taacgcatga cagcagaaga aaaaagcgtc
     7501 ctgtcgcttt tcatgattgg ggtgctgatt gttgtcggca aggtgcttgc cggtggtgaa
     7561 cccatcaccc cgcgtctgtt tatcgggcgc atgttgctcg gtggttttgt ctcgatggtt
     7621 gccggtgttg ttctggtgca gtttcctgac ctgtcactgc ctgcggtgtg cggcatcggc
     7681 tccatgctgg gtatcgccgg ttatcaggtg attgagattg ccattcagcg ccgctttaag
     7741 ggcaggggga aaccgtaatg ccggtaatta acacgcatca gaatatcgcc gcctttctcg
     7801 acatgctggc agtgtccgaa gggacggcga atcatccgct gacgaaaaac cggggctatg
     7861 acgtgatagt caccggactg gacgggaagc cggaaatttt caccgactac agtgaccacc
     7921 cgttcgcgca tggccgaccg gcgaaggtgt ttaaccgtcg cggtgaaaaa tccacggcct
     7981 ccggtcgcta tcagcagctt tacctgttct ggccgcatta ccgcaaacag cttgccctgc
     8041 cggatttcag tccgttgtca caggacaggc tcgccattca gttgatccgc gaacgcggtg
     8101 cgctggatga catccgggcg ggacgcattg agcgcgccat ttcacgctgt cgcaatatct
     8161 gggcgtccct gccgggtgcc ggttacggtc agcgtgagca ttcactggaa aaactggtca
     8221 ccgtctggcg taccgctggc ggcgtaccgg cttaaacgga gtaaacacca tgaagaaatt
     8281 atccctttca ctgatgctga acgtgtcgct ggcgctgatg ccggcactgt ccctgattta
     8341 cccgcagagc gtggccgtca gttttgtcgc cacctgggcg attctggcga tggttatctg
     8401 tgtggttgcc ggtggtgtcg gcgtgtatgc cactgagtat gtgctggaac gctacgggcg
     8461 ggagctgccg ccggaatcgc tggccgtgaa gattgtcacg tcgctgtttt tgcagccggt
     8521 gccgtggcgc agacgggcgg cggctctgat ggtgatggtg gcgacgttta tctcgctggt
     8581 cgctgccggg tggattttta ccgcgctgat ttatctcgtg gcgtcgctgt tcttccggct
     8641 gatatgtacg gcctgccgtc agcgtcttga ggagcgggaa ccatgtcaaa gctgatgatt
     8701 gtgctggtcg tgttgttatc gctggcggtg tccggtctgt ttctggtgaa acacaaaaat
     8761 gccagcctgc gcgcctcgct ggacagggcg aataacgtcg ccagtgaaca gcagacgacc
     8821 atcaccatgc tgaaaaatca gcttcatgtt gccctcacca gggcagacaa aaacgagctg
     8881 gcgcaggtgg cactgcgtca ggaactggaa aacgccgcga agcgtgaagc tcagcgcgag
     8941 aaaaccatca cgagattact caatgaaaac gaagattttc gccgctggta cggtgctgac
     9001 ctgcctgatg ctgtgcgccg gttgcaccag cgcccggcct gcgcagacgc cagtgattgt
     9061 ccacaacgcc tgcccgaaag tgagtctttg cccgatgccg ggcagtgacc cgcagaagaa
     9121 cggcgattta agtgccgata tccggctgct tgagaacgcg ctggcacgct gtgccagcca
     9181 ggtaaaaatg attaaacact gtcaggacga aaacgatgct caaacccgac agcctgcgca
     9241 gggcgctgac tgatgccgtc acggtgctga aaactaaccc cgatatgctg cggatattcg
     9301 tggataacgg gagtattgcc tccacactgg cgacgtcgct gtcattcgaa aagcgttaca
     9361 cgctcaatgt gattgtgacc gactttaccg gtgattttga cctgctcatt gtgccggtgc
     9421 tggcgtggct gcgggaaaat cagcccgaca tcatgaccac cgacgcaggc cagaaaaagg
     9481 gcttcacgtt ttatgcggac atcaacaatg acagcagctt tgatatcagc atcagcctga
     9541 tgctgaccga gcgaacgctg gtcagtgagg tggacggcgc actgcatgtg aagaatatcc
     9601 cggaaccccc gccgccggag ccggtcaccc gcccgatgga gctttatatc aatggcgaac
     9661 tggtgagcaa gtgggatgaa tgagtttaag cgttttgaag accggctgac cggactgatt
     9721 gagtcgctgt caccgtcagg gcgtcggcgg ctgagtgcag agctggcgaa gcgtctgcgg
     9781 cagagtcagc agcgtcgggt gatggcacag aaagccccgg acggcacacc ctacgcgcca
     9841 cgccagcagc agagcgccag aaaaaagacc ggtcgtgtta agcgaaaaat gtttgcgaaa
     9901 cttatcacca gtcgtttttt gcatatccgc gccagcccgg aacaggcatc aatggaattt
     9961 tacggcggga agtcgccgaa aatcgccagt gtgcatcagt tcggtctgtc ggaagaaacc
    10021 cggaaagacg gtaagaaaat tgattatccg gcgcgccctc tgctcggctt caccggtgag
    10081 gatgtacaga tgattgaaga gattatcctg gctcacctcg accgttagtt gtgccattcc
    10141 cgacacctca tcgtcacatt gccgccggta tgacccggcg gcatccttcc cgttatgaac
    10201 actctcgcaa atatccagga actcgcgcgc gcactgcgca acatgatacg ccccggcatt
    10261 atcgtcgaaa ccgaccttaa cgccggtcgc tgccgagtgc agaccggcgg catgtgcact
    10321 gactggcttc agtggctgac ccatcgtgcc ggtcgttcgc gcacatggtg ggcaccttcc
    10381 gtgggggagc aggtgctgat tctggccgtg ggcggtgaac tcgacacggc gttcgttctg
    10441 ccggggattt attccggcga taaccccgcg ccgtctgcgt cggcggatgc cctgcatatc
    10501 cgtttccctg acggggcggt gattgagtat gaacccgaaa ccagtgcact cacggtaagc
    10561 ggaattaaaa cggccagcgt gacggcttct gattctgtta ctgccacggt gccggtggtt
    10621 atggtgaaag catcaacccg cgtcaccctg gacacaccgg aggtggtctg caccaacagg
    10681 ctgattaccg gcacgctgga agtgcagaag ggcgggacga tgcgcggcaa cattgaacac
    10741 accggcggtg aactctcatc aaacggtaag gtactgcata cccacaaaca cccaggcgac
    10801 agcggcggca caaccgggag tcctctatga cagcgcgtta tctcggaatg aatcgcagtg
    10861 atggcctgac tgtcactgac cttgagcata tcagccagag tatcggcgat atcctgcgca
    10921 caccggtcgg ctcacgggtg atgcgtcgtg attacggctc gttgctggcg tcaatgattg
    10981 accagccgca gaccccggcg cttgagttgc agattaaggt cgcatgttac atggcggtgc
    11041 tgaaatggga accccgcgtc acgctgtcat ctgtcaccac ggcgcgcagt tttgacgggc
    11101 gaatgacggt cacgttaacc ggccagcaca acgacaccgg ccagccactt tcgttaacca
    11161 tccctgtgag ttgaaaccat gccgattatc gacctgaacc agctacccgc accggatgtg
    11221 gtcgaggagc tggactttga aaccattctc gccgaacgca aggcgacact gatttccctt
    11281 tacccggaag accagcagga ggcggtcgcc cgtaccctga cgctggaatc tgagcctctc
    11341 gtcaaactgc tggaggaaaa tgcttatcgt gagcttatct ggcgtcagcg tgtgaatgag
    11401 gctgcacggg cggtgatgct ggcctgtgca gccggtaatg accttgatgt gattggtgcc
    11461 aattacaaca ccacgcgcct gattatcacc ccggcagatg attcgaccat cccgccgaca
    11521 ccggcagtga tggaatctga caccgattat cgtctgcgta ttcagcaggc gtttgaaggt
    11581 ttaagcgtcg ccgggtcggt gggtgcctat cagtatcatg gccgcagtgc cgacgggcgt
    11641 gtcgcggata tctctgtcac cagtccgtct ccggcctgcg tcaccatctc tgtgctgtca
    11701 cgtgagaata acggtgtggc atccgaagac ctgctggctg tggtacgtaa cgcccttaat
    11761 ggcgaggatg tcaggccggt ggccgaccgc gtgaccgtgc agtctgccgc catcgttgaa
    11821 taccagataa acgccacgct ttacctttac cctggtcccg aaagcgaacc catccgcgcg
    11881 gccgcagtaa aaaaactgga agcgtatatc acggcacagc accggctggg gcgcgacatc
    11941 cgtctgtctg ccatttatgc cgctttgcat gtggaaggcg tgcagcgtgt cgaactgact
    12001 gcaccactgg ctgacatcgt gctcaacagt acgcaggcgt ctttctgtac cgaataccgc
    12061 gtcgtgaccg gaggctcgga tgagtgattc gcgactgctg ccgaccggct catcaccgct
    12121 tgaagttgct gccgcaaaag cctgtgcgga aattgaaaaa acgccggtca gtattcgtga
    12181 gctgtggaac ccggatacct gtccggcaaa tctgctgccg tggctggcgt gggcgttttc
    12241 ggtcgacagg tgggatgaaa agtggccgga agcgacaaaa cgcgccgtta tccgcgatgc
    12301 gtatttcatt cattgtcata aaggcactgt cggcgcaatc cggcgtgtgg tggagccgct
    12361 cggctatctc attaacgtaa aggaatggtg ggagacaaac gacccgcccg gaacctttcg
    12421 ccttgatatc ggtgtgctgg aaagcggtat cacggaggag atgtatctgg aaatggaacg
    12481 gctgattgcc gatgccaaac ccgcaagtcg ccatcttatc ggtctgaaca ttatccagga
    12541 cattcccggc tatctgtata caggcggtgt ggtctgtgat ggtgatgtta ttactgttta
    12601 tcccggataa gtgagaaaca atgagcacga aatttaaaac cgttatcact actgccggag
    12661 ccgcaaagct ggcagccgcc actgtccccg gcgggaaaaa agtaaacctg tctgcaatgg
    12721 ccgtgggtga cggtaatggc aaattgccgg tgccggatgc cggtcagacg aaactggtgc
    12781 atgaggtctg gcgtcacgct ctgaataaag tcagcgtgga taacaagaat aaaaactata
    12841 tcgtggctga actggttgta ccgcccgaag tgggcggctt ctggatgcgt gagcttggtc
    12901 tgtatgacga tgccggaaca ctgattgcgg tatccaacat ggcagaaagc tataagccag
    12961 aactggctga aggctccgga cgtgcgcaga cctgccgcat ggttattatt ctcagcaacg
    13021 tggcgtccgt tgagctgagt attgatgcca gcacggtgat ggcgacgcag gattacgtcg
    13081 atgacaaaat cgcagagcat gagcagtccc gccgccatcc tgacgccacg ctgacagaaa
    13141 aaggttttac tcagttaagc agtgcaacaa acagcaccag tgaaaagctg gcggcaacgc
    13201 caaaagcggt caaggcagca aatgacaacg caaattcacg tctggcgaaa aatcagaacg
    13261 gtgcagatat ccaggataaa tcagcttttc tggacaatat tggcgttacc agcctgacgt
    13321 ttatgaaaca caatggaatg attccaacca ctgacaatct tgactcctat ggcccagaag
    13381 aaaaatatct tggaacgtgg tcatgtccgt cacaatccac tgcaaaacca gagtctggtt
    13441 accctgaaga taaaggcaat ggggttctgg aagtattcaa tgctggtcgc tttcactgca
    13501 cacaacgcta caccacccga accggtaata tttacattcg gatgcttgat gcagaatgga
    13561 acccagccag tccaacatgg agtgcatgga gagttattac aagcgggact cgaccattaa
    13621 gcacgtcaat tgacctgaat tctttaggtg gcgcggaaca cctgggaata tggcgcaata
    13681 gcagtacatc aatagcctca tttgaacgtc atttcccgga ggacgggagc tttggtcagg
    13741 gtattctgga agtctttgaa ggcggtctgt atgggagaat gcaacgttac accacccgta
    13801 gtgggactat gtacattcgc ggactgactg cctcatggga tgcagagaat ccacaatggg
    13861 aggactggat tgctgttggg tatcagtcca cgggatggac ttattcaggg gatcttgatg
    13921 atcttttaaa gcctggtatt tatagcgtca cgaaacaggc cacaaatgca ccagtcaccg
    13981 actctaaaga tcttgctgtt ggctcaatag ttgaagtgaa aaaacgttgt gatattgaat
    14041 cgtatatcca gacatatacg acagtttctg caacggatgc ctataaaaac cggacatttc
    14101 aacgaactcg tgccagcggt gaagctgact ggggggaatg ggcggaagtt tacaactcaa
    14161 aatcattact cactaaatta ggtgtcggcg gtgttaccga caggttatcc agtctggact
    14221 ggcagaccta cgactttgtg ccgggcagca tgataactgt cagactctcc gatatgacca
    14281 atatccccga tgggatggaa tggggcgtca ttgatactaa cctgataaat atcactgttg
    14341 gccccagtga aggtggaggt gtcgcccgtt caatgcaggt atggcgcagc acctcaaaca
    14401 aaaccaacta tcgttttttt acagtccgct tatatggcaa tcctggggag cgtagcttca
    14461 atattcgtcg gctaccaatt atcgacgaag cccagacatg ggaggcaaaa cagacattca
    14521 gtgcaggcct ctctggtgaa ctgtccggca atgcggcgac agcaacaaag ctgaaaacag
    14581 cccgtaaaat taataacgtt tcgtttgatg gaacatcaga tattaacctg acgccgaaaa
    14641 atattggtgc atttgcttca ggaaaaacag gagacaccgt tgcgaatgat aaagccgttg
    14701 gatggaactg gagtagcgga gcctataacg caactactgg tggggcatca acgttaattc
    14761 ttcattttaa tatcggtgaa ggaagttgtc ccgcagccca gttccgcgtt aattataaga
    14821 acggcggtat tttttatcgt tctgctcgtg atggttacgg attcgaggct gactggtctg
    14881 agttttatac cacaacgcga aaacctacag cgggagatgt cggtgcactg tcgttatctg
    14941 gtggtcaatt gaatggtgca ctgggcatcg gaacatccag tgctcttggc ggtaattcga
    15001 ttgttttggg tgataatgac acgggcttta aacaaaatgg tgatggtaat ctggatgttt
    15061 atgctaatag cgtccatgtt atgcgctttg tctccggaag cattcaaagt aataaaacca
    15121 taaatattac ggggcgtgtt aatccttcgg attacggtaa ctttgattcc cgctatgtga
    15181 gagatgtcag acttggcact cgtgttgttc agaccatgca gaaaggcgtg atgtatgaaa
    15241 aatcaggcca tgtaatcacg gggcttggta ttgtcggtga agtcgatggt gatgaccccg
    15301 cagtattcag accaatacaa aaatacatca atggcacatg gtataacgtt gcgcaggtgt
    15361 aaattatgca gcatttaaaa aacattaagt caggtaatcc aaaaac
//
LOCUS       DAZFRG010000110        14940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFRG010000110 DAZFRG010000000
VERSION     DAZFRG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.110"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..431
                     /gene="yefM"
                     /locus_tag="ACHKUC_002740"
     CDS             180..431
                     /gene="yefM"
                     /locus_tag="ACHKUC_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO6618976.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            428..682
                     /gene="yoeB"
                     /locus_tag="ACHKUC_002741"
     CDS             428..682
                     /gene="yoeB"
                     /locus_tag="ACHKUC_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO6618977.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            765..1589
                     /gene="yeeZ"
                     /locus_tag="ACHKUC_002742"
     CDS             765..1589
                     /gene="yeeZ"
                     /locus_tag="ACHKUC_002742"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6618978.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1635..2564
                     /gene="yeeY"
                     /locus_tag="ACHKUC_002743"
     CDS             1635..2564
                     /gene="yeeY"
                     /locus_tag="ACHKUC_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6618979.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            2779..2841
                     /gene="yoeI"
                     /locus_tag="ACHKUC_002744"
     CDS             2779..2841
                     /gene="yoeI"
                     /locus_tag="ACHKUC_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO6618980.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            2831..4189
                     /gene="plaP"
                     /locus_tag="ACHKUC_002745"
     CDS             2831..4189
                     /gene="plaP"
                     /locus_tag="ACHKUC_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO6618981.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4263..5690)
                     /gene="sbcB"
                     /locus_tag="ACHKUC_002746"
     CDS             complement(4263..5690)
                     /gene="sbcB"
                     /locus_tag="ACHKUC_002746"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO6618982.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPVQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTTWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            5899..7065
                     /gene="dacD"
                     /locus_tag="ACHKUC_002747"
     CDS             5899..7065
                     /gene="dacD"
                     /locus_tag="ACHKUC_002747"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO6618983.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLNRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7184..7657
                     /gene="sbmC"
                     /locus_tag="ACHKUC_002748"
     CDS             7184..7657
                     /gene="sbmC"
                     /locus_tag="ACHKUC_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO6618984.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            7855..8913
                     /gene="yeeA"
                     /locus_tag="ACHKUC_002749"
     CDS             7855..8913
                     /gene="yeeA"
                     /locus_tag="ACHKUC_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6618985.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            9085..9414
                     /gene="tmaR"
                     /locus_tag="ACHKUC_002750"
     CDS             9085..9414
                     /gene="tmaR"
                     /locus_tag="ACHKUC_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO6618986.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9514..9696)
                     /locus_tag="ACHKUC_002751"
     CDS             complement(9514..9696)
                     /locus_tag="ACHKUC_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO6618987.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVKNN
                     GVTELFALLHTEEGCR"
     gene            9750..9896
                     /locus_tag="ACHKUC_002752"
                     /pseudo
     CDS             9750..9896
                     /locus_tag="ACHKUC_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(10185..10265)
                     /locus_tag="ACHKUC_002753"
                     /pseudo
     CDS             complement(10185..10265)
                     /locus_tag="ACHKUC_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10311..10505)
                     /gene="yeeW"
                     /locus_tag="ACHKUC_002754"
     CDS             complement(10311..10505)
                     /gene="yeeW"
                     /locus_tag="ACHKUC_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6618988.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDGRDSTETPATYASPHQLL"
     gene            complement(10502..10876)
                     /gene="cbtA"
                     /locus_tag="ACHKUC_002755"
     CDS             complement(10502..10876)
                     /gene="cbtA"
                     /locus_tag="ACHKUC_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO6618989.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10965..11333)
                     /gene="cbeA"
                     /locus_tag="ACHKUC_002756"
     CDS             complement(10965..11333)
                     /gene="cbeA"
                     /locus_tag="ACHKUC_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO6618990.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            complement(11407..11628)
                     /gene="yeeT"
                     /locus_tag="ACHKUC_002757"
     CDS             complement(11407..11628)
                     /gene="yeeT"
                     /locus_tag="ACHKUC_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6618991.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11697..12173)
                     /gene="yeeS"
                     /locus_tag="ACHKUC_002758"
     CDS             complement(11697..12173)
                     /gene="yeeS"
                     /locus_tag="ACHKUC_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6618992.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKCALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(12189..12662)
                     /locus_tag="ACHKUC_002759"
     CDS             complement(12189..12662)
                     /locus_tag="ACHKUC_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6618993.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLYEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12925..13746)
                     /locus_tag="ACHKUC_002760"
     CDS             complement(12925..13746)
                     /locus_tag="ACHKUC_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6618994.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(13967..14377)
                     /locus_tag="ACHKUC_002761"
     CDS             complement(13967..14377)
                     /locus_tag="ACHKUC_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618995.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKAQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            complement(14393..>14940)
                     /locus_tag="ACHKUC_002762"
     CDS             complement(14393..>14940)
                     /locus_tag="ACHKUC_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618996.1"
                     /translation="FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAA
                     SVKQALSCDPNARAEQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDG
                     PGGNVTPVTINGTVWLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVW
                     FGEIGVHEFAYLVKNYVAAVN"
BASE COUNT         3611 a         3599 c         3870 g         3860 t
ORIGIN      
        1 ttaatgaatg agcggggaaa ggagggggct aagaggactg tcctctaact tgacagcacc
       61 aaaaagagca aagtctgatt tttcgtctcg ctgacaatgt cttgatctac aaactaatta
      121 ataaatagtt aattaacgcc catcattgta caatgagctg tacaaaagag gagatttaca
      181 tgcgtacaat tagctacagc gaagcgcgtc agaatttgtc ggcaacaatg atgaaagccg
      241 ttgaagatca tgccccgatc ctcattactc gtcagaatgg agaggcttgt gttctgatgt
      301 cactcgaaga atacaactcg ctggaagaga cggcttatct actgcgttcc cccgctaacg
      361 cccggagatt gatggactca atcgatagcc tgaaatcagg caaaggaacg gaaaaggaca
      421 ttattgagtg aaactaatct ggtctgagga atcatgggat gattatctgt actggcagga
      481 aacagataag cgaattgtta aaaagatcaa tgaaattatc aaagatacac gcagaacacc
      541 atttgaaggt aaggggaagc cagaacccct gaaacataat ttgtcaggct tctggtcccg
      601 acgcattaca gaggagcacc gtctggtata cgcggttacc gacgattcac tgctcattgc
      661 agcatgtcgt tatcattatt gaacagattc attcatagaa tgaattgtca gttttgatac
      721 gctagcgtag ccttatagat ctaaggtgta tcaggagata acggatgaaa aaggtcgcaa
      781 ttgtcgggtt agggtggtta ggcatgccgc tggcgatgtc actttcagcg cgaggctggc
      841 aagtcaccgg gagtaaaacc acacaagatg gtgtcgaagc ggcccgaatg agtggcattg
      901 atagctatct gcttcgcatg gagcctgagt tagtttgcga ttctgacgat ctggatgccc
      961 ttatggatgc cgatgcgctg gtcattacgc ttccggcacg tcgtagcggc cccggcgatg
     1021 agttctattt acaagcggta caagagttag tggatagcgc gctggcccat cgtattcccc
     1081 gcattatttt taccagctcg acatctgtct atggcgacgc gcaaggcacg gtgaaagaaa
     1141 ccaccccgcg taatccggtg accaacagtg gacgagtatt agaagaactc gaagactggc
     1201 tgcacaattt acccggtact tcggtcgata ttctgcgtct tgcgggcctg gtcggaccgg
     1261 gacgtcatcc cggacgcttc tttgccggaa aaaccgcccc tgatggtgaa catggtgtta
     1321 atttagtcca tttagaagat gttattggcg ctatcactct gttgttacag gcacctaaag
     1381 gcggacacat ctataatata tgtgcgccag ctcaccctgc gcgtaatgtt ttctatccgc
     1441 agatggcccg tttattgggg ctggaacctc cgcagttcag aaatagtctg gacagcggca
     1501 aaggtaagat tattgatggc agtcggattt gtaatgaact gggatttgaa taccagtatc
     1561 ccgatccgct ggtaatgccg ctggagtaaa cgatcacgcc agcaggacgc cgcatacact
     1621 gcaagggggc gtgtatgaaa ccactgctgg atgtgttgat gatcctcgat gccttagaaa
     1681 aagaaggcag ttttgcggcg gcgtcagcca aactctacaa gacgccgtcc gctctgagtt
     1741 acaccgttca caagctggaa agcgacctta atattcaatt gcttgatcgt agcggacacc
     1801 gcgctaaatt cacccgtacc ggaaaaatgt tattagagaa agggcgggaa gttctgcata
     1861 ccgtgcgaga actggaaaag caggcgatca aactgcatga aggctgggaa aacgagctgg
     1921 tgattggcgt ggacgacacg ttcccttttt ctctgcttgc gcctctcatt gaagcttttt
     1981 atcaacatca tagcgtgacg cgcctgaagt ttatcaatgg cgtactcggt ggttcatggg
     2041 atgccctgac tcaggggcga gcggatatta tcgttggcgc gatgcatgag ccaccatctt
     2101 ccagtgagtt tggtttctcg cggttgggcg atcttgaaca agtctttgcg gtcgcgcctc
     2161 atcatccgtt agcccaggaa gaagaaccgt taaaccgtcg tatcattaaa cgatatcggg
     2221 cgattgtggt gggggacact gcgcaggcag gcgcttctac ggcctcacaa cttctcgacg
     2281 aacaagaagc cattaccgtt ttcgatttta aaaccaagct ggagctgcaa attagcggcc
     2341 ttggctgcgg ctatttacca cggtatctgg cgcaacgttt tctcgatagt ggcgcgttaa
     2401 tcgagaagaa agtggtcgcc caaactctct ttgaacccgt ctggattggc tggaatgaac
     2461 agaccgcagg gcttgccagc ggctggtggc gagatgaaat tttagcaaat agtgcgatcg
     2521 ccggtgttta tgcaaaatct gatgacggaa aatcagccat ttaaagaaaa attattatga
     2581 caagcctctc attctcttgt catttccccc ccatttaggc acaatgcgcc gctgtcaaaa
     2641 aatgactaaa aaccgacgtt taattagcgt cggttatttt ttgcttcaaa ccaatcattc
     2701 ataccaagag gccgggcttc gtaccggata gatatttact aaaaatcgac agttgttgtc
     2761 gctgaggaat ccaaaaaaat ggggcaattt tttgcttacg cgacggttat caccgtaaag
     2821 gagaatgacc atgtcgcata acgttactcc aaacacctct cgcgtggaat tgcgtaaaac
     2881 gcttacgtta gttccggttg taatgatggg tctagcctat atgcagccga tgacgctgtt
     2941 tgatacattt ggtatcgttt caggcctcac ggatggtcat gtgccgacag cctatgcgtt
     3001 cgcattgatt gcgatcctgt ttacggctct gagctacggg aagctggttc gccgctatcc
     3061 ttctgctggc tctgcataca cttacgccca gaaatccatt agcccgactg tcggctttat
     3121 ggtgggttgg tcttctctgc tcgactatct gttcgcgccg atgatcaaca ttctgctggc
     3181 gaaaatctat tttgaagctc tggtgccttc cattccatcg tggatgtttg tggtggcgct
     3241 ggtggccttt atgaccgcct ttaacctgcg tagtctgaaa tccgtagcga acttcaacac
     3301 tgtaatcgtc gtgttgcagg tagtgctgat cgcggtgatt ctgggcatgg tggtttatgg
     3361 cgtatttgaa ggtgaaggcg cgggtacgct ggcgagcacg cgtccgttct ggtctggcga
     3421 tgcgcatgtt atcccgatga ttaccggggc gacaatcctg tgcttctcct ttaccggttt
     3481 tgacggcatc agcaacctgt cggaagaaac caaagatgca gagcgcgtga tcccgcgtgc
     3541 gattttcctg accgcgctga tcggcggcat gatcttcatc tttgcaactt acttcctgca
     3601 gctgtacttc ccggatatct ctcgttttaa agatccggat gcgtcacagc ctgaaatcat
     3661 gctgtacgtt gcgggtaaag cgttccaggt tggcgcgctg atcttctcca ccattaccgt
     3721 actggcgtcc ggtatggcgg cgcacgcagg cgttgcgcgt ctgatgtacg taatgggtcg
     3781 tgacggcgtg ttcccgaaaa gcttcttcgg ttatgtacac ccgaaatggc gtactccggc
     3841 gatgaatatc atcctggttg gcgcgattgc cctgctggca atcaacttcg acctggtaat
     3901 ggctacggcg ctgattaact ttggtgcgtt ggtggcgttc accttcgtta acctgtcggt
     3961 catctcgcag ttctggatcc gtgagaagcg taacaaaacg ctgaaagatc acttccagta
     4021 tctgttcctg ccgatgtgtg gtgcgctgac cgttggtgcg ctgtgggtta acctggaaga
     4081 aagctcaatg gttctgggtc tgatctgggc tgctatcggt ctgatttacc tggcttgcgt
     4141 aaccaagagt ttccgtaacc cggttccgca gtacgaagac gtagcgtaat cttacaaacc
     4201 ggatgacata atcatccggt aaatctcaaa aagccggagt ttgtcgctcc ggcttttttg
     4261 tttcagacga tctcttccgc gtactgccaa agcgctttta acagcgccac tttctctttg
     4321 tcatcggcat actgttgtgc cagcatctgc aattcatcag cgtagccctg caaaaactct
     4381 ggcgtgaaga cctggcggcg gtgctccagc cagcgttgct gctcggcata atccagcgtc
     4441 cccgggaagt tgcgcgctcg ataattgaac aacagttttt caattcgttt atcaacaaaa
     4501 gtgatatcca gtgccggtaa attacgcggc tcggtttcca gcacaatttt cattgctgca
     4561 cgatcggcgt cactgaaaaa gccattataa agctgtgcat ccacgttctc tgaaggcgta
     4621 aacggttcgg cttccgcgaa tatcgccacc actttttcac gcacttgcgg attttcacgc
     4681 agaattttta ggttatcgag gcaatgctga cgattaattc ccagtcggtc ggcatcttcc
     4741 gggcgtagcg tattcgcctg ggccagtacc ggacatttat tgatatgcac cagcttaacc
     4801 ggaacggcgg cgttatcgcc aagatcggct tttgcggtat ataaacgctc gcgcaatgtg
     4861 tcgctatcca gctccagtaa tggcgaaatg tctcctgcca aatccaccat aattacggca
     4921 ttgcgatttt caggatgcca cgccagcggt gccacccagg tggtattgcc gcgccatgcc
     4981 ccaaacattc cggaaacgtg aaccaggggt ttcatctgcg gaacatcaat caacgccatc
     5041 agtttgtgtt tattacgatg ggtaaagaga taatcaaaca ggcgtggctg acgcgttttt
     5101 accagtttcg ccatcgcaat agtggcgtac acatcagcca tcgcatcgtg ggcgttgcta
     5161 tgttcaatac cattcgcttt ggttaaatgc tcaaggcgaa agctcggtag accgtcatca
     5221 ttttcaggcc agtttattcc ttccgggcgc agggcataac aggcacgcat aacatccagt
     5281 aaatcccagc gcgagttatc atgctgccag ctccaggcgt aaggatcgta gaaattacga
     5341 taaaaaacgt tgcgtgtgac ttcgtcgtcg aaacgcacat tgttgtagcc cagaatacag
     5401 gtcttcggta cggtaaaaag cgagtgaata cgggcggcaa acgcggcttc gttttctcct
     5461 ttcgcccgtg cctcctgcgg ggtaataccg gtaattaata ctgctccagg ctggggtaaa
     5521 tagtcatccg cgggcttgca gtaaaagact tcgggttcgc cgatgacatt gaattcgtta
     5581 tcagtgcgaa tggctgcgaa ctgtacaggg cgatctaacg cggggtgcgt tccaaaggtt
     5641 tcgtaatcgt gaaacaaaaa ggtagattgt tgcttaccgt cattcatcat taggttaaat
     5701 ccgttatttc tgctgtctgc cagagtatca aatatcaccg tgctaatcag ctttagcgcg
     5761 acaatttggc agcgagtggc aagagttcat gtcagataaa aatgagatag tagtcacatt
     5821 ttcttgcact ttattccagc cagttcataa gtatttccgt aaaaagaaca gctatttgaa
     5881 acacctgagg gtttgctgtt gaaacgccgt cttattattg ctgcttcttt gttcgttttt
     5941 aatttatcgt ctggttttgc ggcggaaaac attccttttt cacctcaacc tccagagatt
     6001 catgccgggt cctgggtatt gatggattac accaccggtc agattctcac cgcgggtaat
     6061 gagcatcaac agcgcaatcc cgccagcctg acaaagctga tgacgggcta tgtcgtggat
     6121 cgcgctatcg atagtcatcg cattacgccg gacgatattg tcaccgtggg gcgtgatgcg
     6181 tgggcgaaag ataatccggt gtttgtcggt tcttcactga tgtttttgaa agagggcgat
     6241 cgcgtatcgg tacgtgattt aaaccgtggt ttaattgtgg attccggaaa tgacgcttgt
     6301 gttgcactgg ctgactatat tgccggtggg caacggcagt ttgttgaaat gatgaacaac
     6361 tatgccgaga agctgcatct caaggatacg cattttgaaa cagtgcatgg tctggatgca
     6421 cctggccagc atagctcggc ttatgattta gctgtgcttt ctcgcgctat catccacggc
     6481 gagcccgagt tttatcatat gtacagtgag aaaagcctca cctggaacgg tatcacccag
     6541 caaaaccgta acgggttatt gtgggataaa accatgaatg ttgacggcct gaaaacgggt
     6601 catacttctg gtgccgggtt taatctcatt gcttcggctg tagatgggca gcgtcgtctc
     6661 attgcagtgg taatgggggc tgacagtgca aaaggtcgtg aggaagaggc aagaaaatta
     6721 ctgcgttggg ggcaacaaaa ctttactacg gtgcaaattt tgcaccgggg gaaaaaggtc
     6781 ggtacggaac gcatctggta tggcgataaa gaaaatatcg ccctgggaac ggaacaagag
     6841 ttctggatgg tgctaccgaa agccgaaatt ccacatatca aagccaaata tacccttgat
     6901 ggtaaagaac tcaccgcgcc aattagcgcc catcagcggg taggggaaat tgaactttac
     6961 gaccgtgata aacaggtggc gcactggccg ctggttaccc tggaatctgt cggggaaggc
     7021 agcatgtttt cccgcctgag tgattatttc caccataagg cctgaccttt cttttgcagc
     7081 agactggcag gagtgcgagt ctgctcgcat aatcaacact cattccttgt ggttttaata
     7141 ttgcaactat actgtatata aaaacagtat tagtggaggc gtcatgaact acgagattaa
     7201 gcaggaagat aaacgtaccg ttgcaggttt ccatcttgtt ggcccgtggg agcagacggt
     7261 aaagaaaggt tttgagcagt tgatgatgtg gatagatagc aaaaatattg tgccgaagga
     7321 gtgggttgct gtctattacg acaatccaga tgaaacaccc gccgaaaaat tacgctgcga
     7381 caccgtcgtg acggtaccga ataactttac gctccccgaa aacagtgagg gcgtcattct
     7441 gacagaaatt tcaggtggtc agtatgcggt ggcggtggct cgtgtagtcg gtgatgattt
     7501 tgctaaaccc tggtatcagt tctttaatag cctcttgcag gacagtgctt atgaaatgtt
     7561 accaaagccc tgcttcgagg tttacttgaa caatggcgcg gaagatgggt actgggatat
     7621 cgaaatgtat gttgcggtgc agccaaagca tcactaattc atctcagggc ggtgtgttaa
     7681 cgcgaagacc actctttttt tgaaagcgaa aagagtaaga tgcgcctttc aaatttttcg
     7741 ctcctgccgg gaaattacac tgttcccggt atgtccgtcg gataactcag aggcgcgcct
     7801 tctggccgac agatgtgtta tgagcgcttt taatcttcat tacggagttt ctgcgtgcgt
     7861 gccgataagt cattaagccc gtttgaaatc cgggtatacc gccattaccg cattgtgcat
     7921 ggtactcggg tcgcgctggc attcctgctc acttttctca ttatccgcct gtttactatc
     7981 ccggaaagca cctggccgct ggtcaccatg gtggtgatta tggggccaat ctcgttctgg
     8041 gggaacgttg tccctcgtgc cttcgaacgt attggcggta cggtgttagg ttcgatttta
     8101 ggtcttatcg ctctgcaact ggagttaatt tcgttaccgc tgatgttagt ctggtgcgcg
     8161 gcggcgatgt ttctttgcgg ttggctggcg ctgggcaaga aaccgtatca aggattattg
     8221 attggggtga cgctggcaat tgttgtgggt tccccgacag gtgaaattga tacggcgtta
     8281 tggcgaagcg gcgatgtgat cctcggctct ttactggcaa tgttgtttac cggtatctgg
     8341 ccacaacggg cgttcatcca ctggcgcatt caactggcga aaagtctgac cgagtataat
     8401 cgggtctatc aatctgcatt ctcaccgaac ttactcgaac gcccacgtct ggaaagccat
     8461 ctacaaaaac tcctgaccga tgccgtgaaa atgcgtgggc tgattgcgcc cgccagcaaa
     8521 gaaacccgta ttcccaaatc gatatatgaa ggtatccaga ccattaatcg caatctggtt
     8581 tgtatgctgg agttgcaaat caatgcatac tgggccacgc gccccagcca tttcgtgtta
     8641 ttgaacgcgc aaaaacttcg tgatacccag cacatgatgc agcaaatact gctgagcctt
     8701 gttcatgcgc tgtacgaagg taatccgcag ccggtttttg ccaatacgga aaaattgaac
     8761 gatgctgtgg aagagctgcg tcagttgctc gataaccacc atgacctgaa ggttgtggaa
     8821 acaccaatct atggttatgt gtggctgaac atggaaacgg cacatcagct tgagttgcta
     8881 tcgaatctga tttgccgggc cttgcgcaaa taattcctga acttcagaat catcttgctg
     8941 ctgcttcgat tcagcaagga taaagggtat gatagtgaaa agggataaaa gcattgtcat
     9001 ctgcggcagc tatgagtaat gttggcccta acgaatagcg gttgcttaaa cgaatccgac
     9061 tctcacatta tcaggggtat aaaaatggaa actaccaagc cttcattcca ggacgtactg
     9121 gaatttgttc gtctgttccg tcgtaagaac aaactgcaac gtgaaattca ggacgttgag
     9181 aaaaagatcc gtgacaacca gaagcgcgtc ctgttgctgg acaacctgag cgattacatc
     9241 aagccaggga tgagcgttga agcaatccag ggcatcatcg ccagcatgaa aggtgactat
     9301 gaagatcgcg ttgacgatta catcatcaaa aatgccgagc tctccaaaga acgccgcgat
     9361 atctccaaaa agctgaaagc tatgggcgaa atgaaaaacg gcgaagcgaa gtaattcctg
     9421 ttttattcaa tgagggttgc ccggcaaccc tcattgctca ttgattctta tctgttatca
     9481 ccgtcatcat tctcatctga gaacctatcg aaattagcga cagccttctt ctgtatgcag
     9541 caaggcaaaa agttctgtaa ctccattgtt tttaaccgca ctggttacta acacgttgtg
     9601 cgctccagct tcccgtaacc aacatttcac caaagatatt tgttccatac tggctaaatc
     9661 tgctttggtt actactccaa tgaccgggtg attcatggcc cggtagggcg tttttacctc
     9721 taattcctgc ttcagcgctg agattcctgc ttgttgccgg aaaagcaggc acctgccgtc
     9781 tgaacggtat cgatccggaa gcgtatctgc gccatattct gagcatactg ccggaatggc
     9841 cctccaaccg tgttgacgaa ctcctgccat ggaacgtagt tctcaccaat aaataagcgt
     9901 caatacggtg ctccgttgac gcttacttta atccgttttc tgagcttaga tttttctgtt
     9961 cctacccaga ccgtggattt ggaattcatt ttttgtcagg tcaatgctaa tcgttaaaac
    10021 ggccataacg cacctccact ggaaggttta tcagtaatat cctaccgttg cttcatggtc
    10081 ggggcgtcca ttccattatt atcggttttc ctttctgttt ataaatgaaa acgccagctg
    10141 tattcaggct ggcgtcaggg gaaatgaagc ctgttgagtg agattcaccg gttctggtgc
    10201 agaagctgca gatggcgcag ggtaaggaca tcttctcttg cctgcttaat acaggacaac
    10261 agtgctgttt ctgtggttaa tggcactata ccctgtatca ctgcattgtc ttacagcaac
    10321 tggtgagggc tggcataagt tgccggtgtt tcggtagagt cacggccatc caccacgcgg
    10381 agtgaataac cgttttcagc ggccttatta atcagttcag tgatattaac accatcaatg
    10441 tcaacgacaa tgtgccccat atccagcgcc tgtacgttaa cgctgtcggc ttccagtgtc
    10501 atcatttcgc ctccggatac ttacccaggg taatgttatt taccgttctg taattgtcgc
    10561 gggtcatcag gccggtcgcc ctgcgagccc ggaggatatc gatgctgttt attaactgag
    10621 agcgggtaca ggcgctgaat cccggctggt cggtacgcac cagcgcgtat ttttccacga
    10681 gaaagttcac cgcatcacac agtgaaatgc ctgcctcaat atgctgctca atcacacgtt
    10741 catcggcaaa cggtgtgtca ttcagtgtga ggccgtagtg ctggtccagc agtcgggaca
    10801 gcagtatctg ccagatttca acaggagacg ggcgagaact ggccgcctgc ccgggtaata
    10861 caggtaatgt tttcatactg aagattttcc tgatatgcag atataaaaat gggaaagttg
    10921 cgtggtgaaa acaccaggcc gtagcagaag gctattctgg agagttaatt tttcatttcg
    10981 ggcgtcggat aaacagccag ataaacgtaa ccacaggagc ccagggtatc ggcttcgcag
    11041 gtcagtcctt tcgcatacag cgtgacggta tgctgatagc gggggctgag ttcaccactg
    11101 gtgagcatga gctccagttg tttcatcagc aacggaaagg cctggtccag gtggtacgca
    11161 tctgcatcgc tgaacaggcc tctgataccg gcacggtcgg caaggtaatg caaccggtta
    11221 ccctcctgca ccagacgggc gccgaaacag ggcgtcacgg tgcagggtag cccccaccag
    11281 gggcggtcgt gattgtcgtc gggaagtgtt gtcccgggga gtgtgtctga cacgataaaa
    11341 tccctacaga aaatcggtta agaatggtcc ggtggtggcg ataattctgc tcatcagaat
    11401 tcccactcag ttcagggtga cgctcatcag tcggacatac gggccaaaac tgtccttacg
    11461 gcgttcagca aacacggcca gcacaccggg aatatcctgc acttcacgac cggtatacgc
    11521 ttcagcactg ccgtgccagc ggtatttacc ggtacagaac ggaaaaagac gggatgtcgg
    11581 atgttgttgg tgaatacgca tggcttcacc acgggtgata attttcataa taggatacct
    11641 ttgaagacag aagataaaag tgaaaacagg tgtgatgtga ttgtggcggt gacgggttaa
    11701 agcaggccgt gttccgcaaa ggagaaaacc tggctgccac cgactatcag atggtccggc
    11761 acccggatat ccaccaggcc cagtgcctgt accagacgtt cggtgataag ccggtctgcc
    11821 ttactgggtg tgacttcacc ggacgggtga ttgtgtgcca gcaccacggc tgcggcattg
    11881 tgatacaggg cgcatttaat cacttcccgg ggatggactt ccgtgcggtt gatggtgccg
    11941 gtgaagaggg tttcaccggc aatcagctga ttctggttat tcagatatag cacccggaat
    12001 tcttcacgct ccagtcccgc catgttcaga atcagccatt cccgtgccgc acgggtggag
    12061 gtgaaggcca caccgggttc atgaagatga cggtccaggg ttttcagggc ccgcagaatg
    12121 agactgcgct cgccgggcgt catctctccg ggcagaaagg aaagttgttg cattgtactt
    12181 ctctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
    12241 tcccgcagac ggtaataatg aaccgtcatg gcataacact ccgtacggca ggcgtgatga
    12301 ctgtacgtca tcagacaggc agcaatgccg gcggcttccg ggctcatttc agcgcggtta
    12361 ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc
    12421 ataaatgcgc caccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttca
    12481 tacagacggt ccatccagcc gaagacccgg ggctccaggg tcacccactg tggaatgagg
    12541 ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag ggactatggt ggcaaccagt
    12601 tgttgcaggc cagtctccgg tgcacaggcc gaagaggtgg tgttctgaga caatgttttc
    12661 atgtatgaaa tccttgtaat gtgaataaat gagagggagt aaaggccggg ggctgacaac
    12721 atcacgttca tcactgaccg tgagacccgg taatgtcagg gatgaatgat attcggctga
    12781 aattccggac agccgtacgc gtaaccgttc cggtcacaga cggatggcca gatgccattg
    12841 cctgtactca gtctgcacag actgagctct ccggggcgtc gggtgattct ggtgcctgaa
    12901 tcacgccttc agggacagat tgcatcagcg caggctctcc agcatcgttt ctgccatcac
    12961 ccacaatgca cggttgagct taatgtcggt atcaatgctg tgaatggccc gggtatggat
    13021 acgttttcct tttgcactgc gaccggaaat cccgcctttc agcatattct cctggatggt
    13081 ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggcg tcagaatgtc
    13141 ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag
    13201 cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct tctcctctat
    13261 ccggtcaaac acgcccacca cctcgtaagc cccttcaata actttctcca ctacgtctcc
    13321 ccggtgtgga acacgcactt cccccagaga ctgaccacag acgcagccgt tctggcagac
    13381 gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatgag agttgagcag
    13441 aataatttca gggacatgct ctccgtttat ctctccggcc cgccgcagac gcagcatgtg
    13501 tttggtgtat ccccggcggc ccgggtcgcg cacacgggtc tggcaggcga agaatggctg
    13561 aaagccttcc cgctgcaggc tttccagtac ggtgatggtg gggatgtacg tatagttctg
    13621 gcttcgggac gtgtgtttct cttccccgaa aatccccggc acatggtgca tcagctcttc
    13681 atgtgtcagt ggacggtcac gacgtatctg gtttgcagca taaccaaaac gacttgctaa
    13741 tcgcatactt tgctccttat cggtggttaa gatttactgg tgtaataaat gaaaaaagcc
    13801 acgtctcccg gagaagacgc ggcctgacgg gtgaaatgaa tgaggtttat tgtctgagaa
    13861 gcctttcact ggcgagccac gcattaagcg gctttccggc aacaatggct ggggctgtat
    13921 ttacctttga tattaataat gtgcagagaa acgcatggtt tactcctcag gttttaagtt
    13981 tgtccagagc cacaacgaca ggttttccgg atgtgtcgat gactcctacc gtcggtacgc
    14041 catttttgat ttcgaagaac ggtttgttgc tacctgagca cagctggttg tctttgtatt
    14101 cgagcatctg tgctttcccg gttttcaggt taagtaccga gacagtgtag tcgtaacacc
    14161 aggcaccacc acgttcaagc acgaaaacat agctgttatc tttatagtta aacggattct
    14221 tcgtctggca attcataccg tcaatctcag tacaggaaaa cgcctctgta gtattaacgt
    14281 atttgatgtt gtttggcagt tttacttgtt tcgccgtagc gaagacagag aacagaatag
    14341 ttacagcaac gagtaaagtt ttgataaaag ttttcatgac agactcctta aattagttaa
    14401 ccgcagctac gtagtttttc accagatacg cgaattcatg cacgccaatt tcgccgaacc
    14461 aaaccaggta ggccgggtca tctaaaccaa agtagcggta gccaacgact tcatgggttt
    14521 ttgggttata gagtgttacg ataaagtccc agccgcaggt attggctttg caaccgtcgc
    14581 cctggagcca gactgtaccg ttaatggtta ctggcgttac gtttccgcca gggccatcca
    14641 tatacccgcc tttaccgaac atgccgctca gtccgaccgg ttttaatgct ttgtcaagcg
    14701 cagctttgta ggcagcgtca ccttcgtaca actcgtacgt tgttggacac gctccaggtt
    14761 gttcagcccg ggcgtttggg tcgcagctga gcgcttgttt cacgctcgct gcttccacgt
    14821 taaagccagt aaacagaacc gccagaaata acatcagggt atgtaaacgt ttcattgtaa
    14881 atattcttcc gttgtaaatg gcgctggaaa atttctcatc agtgaaacct tccggaaatt
//
LOCUS       DAZFRG010000111        14806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFRG010000111 DAZFRG010000000
VERSION     DAZFRG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.111"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            256..597
                     /gene="symE"
                     /locus_tag="ACHKUC_002763"
     CDS             256..597
                     /gene="symE"
                     /locus_tag="ACHKUC_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO6618997.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(644..1807)
                     /locus_tag="ACHKUC_002764"
     CDS             complement(644..1807)
                     /locus_tag="ACHKUC_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO6618998.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSQ
                     EDFLNIIRLIESYVFRRAVCAIQTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPALPEEVLAVYRAQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1861..2737)
                     /locus_tag="ACHKUC_002765"
                     /pseudo
     CDS             complement(1861..2737)
                     /locus_tag="ACHKUC_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2835..2915)
                     /locus_tag="ACHKUC_002766"
     CDS             complement(2835..2915)
                     /locus_tag="ACHKUC_002766"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6618999.1"
                     /translation="MQTRCVLQQVRDERLKDIGLCREDVV"
     gene            complement(3143..4063)
                     /locus_tag="ACHKUC_002767"
     CDS             complement(3143..4063)
                     /locus_tag="ACHKUC_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6619000.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLAIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPDREHDSWLES"
     gene            4248..5528
                     /gene="yjiN"
                     /locus_tag="ACHKUC_002768"
     CDS             4248..5528
                     /gene="yjiN"
                     /locus_tag="ACHKUC_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO6619001.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALILESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLNADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRIAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            5644..6795
                     /gene="yjiM"
                     /locus_tag="ACHKUC_002769"
     CDS             5644..6795
                     /gene="yjiM"
                     /locus_tag="ACHKUC_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO6619002.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            6805..7572
                     /gene="yjiL"
                     /locus_tag="ACHKUC_002770"
     CDS             6805..7572
                     /gene="yjiL"
                     /locus_tag="ACHKUC_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO6619003.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQKF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            7569..7826
                     /gene="ytiA"
                     /locus_tag="ACHKUC_002771"
     CDS             7569..7826
                     /gene="ytiA"
                     /locus_tag="ACHKUC_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO6619004.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYGVSPR"
     gene            7891..8750
                     /gene="yjiK"
                     /locus_tag="ACHKUC_002772"
                     /pseudo
     CDS             7891..8750
                     /gene="yjiK"
                     /locus_tag="ACHKUC_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            8818..9996
                     /gene="yjiJ"
                     /locus_tag="ACHKUC_002773"
     CDS             8818..9996
                     /gene="yjiJ"
                     /locus_tag="ACHKUC_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6619005.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFFGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLSNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(10009..10563)
                     /gene="kptA"
                     /locus_tag="ACHKUC_002774"
     CDS             complement(10009..10563)
                     /gene="kptA"
                     /locus_tag="ACHKUC_002774"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO6619006.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            10813..11496
                     /gene="yjiH"
                     /locus_tag="ACHKUC_002775"
     CDS             10813..11496
                     /gene="yjiH"
                     /locus_tag="ACHKUC_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO6619007.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11493..11954
                     /gene="yjiG"
                     /locus_tag="ACHKUC_002776"
     CDS             11493..11954
                     /gene="yjiG"
                     /locus_tag="ACHKUC_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO6619008.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            11967..13139
                     /gene="iadA"
                     /locus_tag="ACHKUC_002777"
     CDS             11967..13139
                     /gene="iadA"
                     /locus_tag="ACHKUC_002777"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6619009.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13204..14115
                     /gene="hypT"
                     /locus_tag="ACHKUC_002778"
                     /gene_synonym="qseD"
     CDS             13204..14115
                     /gene="hypT"
                     /locus_tag="ACHKUC_002778"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO6619010.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(14108..14500)
                     /gene="iraD"
                     /locus_tag="ACHKUC_002779"
     CDS             complement(14108..14500)
                     /gene="iraD"
                     /locus_tag="ACHKUC_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO6619011.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14497..14580)
                     /gene="idlP"
                     /locus_tag="ACHKUC_002780"
     CDS             complement(14497..14580)
                     /gene="idlP"
                     /locus_tag="ACHKUC_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO6619012.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(14696..>14806)
                     /gene="ytiD"
                     /locus_tag="ACHKUC_002781"
     CDS             complement(14696..>14806)
                     /gene="ytiD"
                     /locus_tag="ACHKUC_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO6619013.1"
                     /translation="PELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         3580 a         3779 c         3628 g         3819 t
ORIGIN      
        1 ttgcagacgc actgactaac gcggcgataa actaattttc ccgccctcaa aaaagcaata
       61 aagcggctca atagccgctt tattcacatc agcaaaaatt atatttcacg ctaattttca
      121 tcttattttc attaattttt agctggactt tccctgtatt tactgatgat atgtccaggt
      181 atttagcgcg gtgcggatgt gcgccaacac acccgcaccg ctaatcacaa tcactattta
      241 tagggaatag cagttatgac tgacacgcat tctattgcac aaccgttcga agcagaagtc
      301 tccccggcaa ataaccgtca attaaccgtg agttatgcga gtcgctaccc ggattacagc
      361 cgtattcccg ccatcaccct gaaaggtcag tggctggaag ccgccggttt tgccaccggc
      421 actgcggtag atgtcaaagt aatggaaggc tgtattgtcc tcaccgccca accacccgcc
      481 gccgccgaga gcgaactgat gcagtcgctg cgccaggtgt gcaagctgtc ggcgcgtaaa
      541 caaaggcagg tgcaggagtt tattggggtg attgcgggta aacagaaagt cgcctgatac
      601 attgacccgg tagcgccagt gctaccgggt attattcgcc acgttatacc aacgcgctct
      661 ccatctgttt ttcaaatgcc tgacgaatta atcccataat gtaaggaagt tgtgcgaggt
      721 cactgaaacc aatttccaca tcaccattgc cccatcgccc aacatttgtc acatctttgg
      781 caataccttt cggatcgacc agttcatgaa actgcatatt aagcgacaac cgcagtcggc
      841 ttttttgcgg tacaacatca acaaaattcg tttcagcttt aaacgcaata tagagcttta
      901 aaacttcctg cgtgatccct gcgtccaggc gcataacttc atcgcgaaga tgatcaaata
      961 acacccggct atgcgaacca tcagcaagaa aaggataatc actcaggctg taactggttt
     1021 tgttctccgg ctgcgcccga taaacagcta aaacctcttc aggaagagca ggcgcttgcc
     1081 agacctgaac cgcgagatcg gccagacgat ctgcacgggc gcgaatggct gcttcatccc
     1141 atttttcgca ctgtccaaga ccgatattca aatagagcgg gctatgttta aagccgtctt
     1201 caatatcgcg tttttccgcg aaaggtctgt cactgtagcg ggagttatag cgcgttaatg
     1261 tgagattccc caacgtatgc aacagttctt tatgaatgcg ttgccagtca cttcccagct
     1321 cttcacgcca tttagccgac aaattttcgt tctgcggcat aatatgctca atcgtaaact
     1381 cttccacgcg ctctctgcgt ttatcgtttt ccaggcgtcg taaccagtag ctgcgactgc
     1441 ggaagttata gagatcgcga acttttaatt cccgtttaaa ttcatcatcg ttggggaaac
     1501 gacgatatga aggcaggttc ataaaatgta cctgaatgct ttccagatat ttttctttat
     1561 taatgacctt ataaaaagtg gcaaacgtct tgttcagaga atttgtctga atcgcacaca
     1621 ctgcacggcg gaaaacataa gattcaatta aacgaattat gttcaggaaa tcttcttgag
     1681 acaaaacgcc atttttatag tcatgataaa gcgccagtaa gaaaggatac gccacatcga
     1741 cctttaactc gcgcaaatcc tgaaaagccg tggcaagcga tttgtcactt tctttaccca
     1801 atgccattgc acagtaatac tccgcgtaga tgtgaatatc ttcaaccagc cgatccacac
     1861 ctttttcggc aacactcggg ctacgagcat gcagtttaaa tgcctcatag acttcatctg
     1921 tccgagggat ctcccccgtt tttaccgtca gataatgacg cataaaactg tcaaaatatt
     1981 cgctgtaacc ttgctgacca aaagcgactt ccattggacg ccagtgatct tcatacaacc
     2041 gggtttgatg ctctggttcg agacccatca gaataaaatt gcgcaccaga tcggcctgac
     2101 ttaacgcctt accagtagag ttcatacttt caaaaatcag ttgcggatta tcctgaccac
     2161 gattaagcgc cacatcgaca attaataact ttgctaaccc acgacataag gggatcaagt
     2221 catcaccgag tttggcaatt tgctcatcaa agaaagtaaa gttttccatt attcgatggg
     2281 aatagttttc tggcattggt ctttgtttta ttaacgccag caaactgtct ttatcagtct
     2341 cagtcagcaa taatttatat cctttctcgc cggactcata aggattgagc aaataataat
     2401 tacgcaattt cattgccgag aagccatcaa aaacttcttc ttcgccaaga tggcgtgata
     2461 aagcctcaat cagcaacatt gcggtcgtca gccgctgttg accatcaatg accagtagcg
     2521 gagagatacc agaaacctga tacaacccct gctcaatata aacaaccgaa ccgataaaat
     2581 gtgctgatat atcgtcacgc tttccggccc gaatgatatc gtcccaaagt tgacgacatt
     2641 gttgcccggt ccatgaatag gtgcgttgat aaatgggaat aacaaactgc tgcgaacatt
     2701 ttaaaaaatc gagtaggcgg gcttctgtag ctttcattct gacgtctttc cgtgaagcga
     2761 tgatttcgca ataataaccc cttatctgac gaggtgacat ataaatccca tcgtttcgtg
     2821 aaggactctc tccattacac cacatcctcc ctgcataacc caatatcctt taaccgttca
     2881 tctctcacct gctgtaacac gcaccgggtc tgcatttgct gccaccagca gcgttaatgt
     2941 accgttcaca taaatggcgg cggcaacgtt ggtgcaggaa ggtcactatc atcgccacgt
     3001 aaagtgtgct tcgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaccgcg
     3061 caaattcaat agattgcaga gataatgtag gtctgataag cgtagcgcat caggcagctt
     3121 tgcgtttgtc atccgtctct gattagctct cgagccatga atcgtgctca cggtcaggca
     3181 agggctgctc taatgcctgt atttgctctg ccgaaagtcc ggtaatcttt tgtatccatt
     3241 caggatctac cccattctgc aaaaacaatc gaagaatgcg ctgttcccct tttatatatc
     3301 catcattatg aattcgctct gcaatcgtca ttattcgctc cctttgttgt ggcagtcgac
     3361 gggtaatttc actgataaac tcattaaaac gcgcttcatc accagtcagt aaaatgtaat
     3421 ttaacagcgc agttatctgg ctgtcattag cacactctgt aaccagtaat actaccagtt
     3481 ggtcaataag ccccatcaga tcgcgctggc gaatatgctt ttggatcaac tccaacaggg
     3541 cgactctgcg atgctgcaca atctcgtcgt ctggcacgac agtgatatcc accagcggga
     3601 acgcggcggt ataaagcttc cgtgcggtag tcgggtcggc aaattcgtcc agccagcaca
     3661 aagaccaggg ataagggcta cggctaccgt gataaaacag catcgggatg gccagcggta
     3721 gctgccggcg tttatcatgt tcgatatggc gctgcatcac cgccatggaa tatcgcatca
     3781 gacgaaaggc catatgaata tcctcacggc tctgatgttc aatcactaca taaatatagc
     3841 catcgccttc acgggtcttt accgaccaca gaatatcgga atgtagcgcc cgcaattttt
     3901 catcgacaaa actggcggat tccagtttta agctatcgag atcgcacagt tcacgtaaat
     3961 ctttgggtaa gtggatctcc ataaaatccc gcgcggtgtc agagtgtgtg agaaaggatt
     4021 taaataatgc gtcatgcggc gtgctggtcg taaagtttgt catggcgatc cgtcacctgt
     4081 agataagatg gcccaacttt agcgactaac aaagccattc acacctggct atttcctatt
     4141 ccccgaggcg gtttccaggc tatttttatt cttctgcaaa cgatacattt ttatggaaga
     4201 tatctcgctt tttgcgtttt cttaccgtca ggctctattc ttacgatatg aataaactca
     4261 ttgaactcag acgcgccaaa atgttggcgc tctctttact gcttatcgcc gctgctacct
     4321 ttgtcgttac gctgtttttg ccgcccaatt tttgggtgag cggcgtgaag gcgattgctg
     4381 aagcggcgat ggtcggcgca ctggcggact ggtttgcggt ggtggcgctg tttcgccgcg
     4441 tgccgattcc gatcatttct cgtcatacgg cgattatccc gcgtaataaa gaccggattg
     4501 gcgaaaatct cggccagttc gtgcaggaaa aatttctcga tacccaatcg ctggtggcat
     4561 tgattcgacg tcacgaaccg gcgttgttga ttggcaactg gtttagtcag ccggagaacg
     4621 cccgccgcgt tggtcagcat ctgttacaga tcatgagcgg ttttctcgaa ctgaccgatg
     4681 atgcgcgtat tcagcgcctg cttaagcgcg cggtccaccg ggcgatcgat aaggtcgatc
     4741 tttccggcac cagcgcactg attctggaga gcatgaccag aaacgatcgt catcaggtgc
     4801 tgctcgatac gctgatcgca cagttgatcg cccttctcca gcgcgataaa tcgcgcaagt
     4861 ttatcgccca gcaaattgtt cgctggctgg agagcgagca tccactgaaa gccaaaattt
     4921 tgcccactga atggctgggc gaacatagcg cggagttggt ttctgacgcg gtgaattctt
     4981 tgcttgatga tatcagccgc gatcgtgcgc atcagatccg ccatgcgttt gatcgcgcca
     5041 cttttgccct gatcgacaag ctgaaaaacg atccggaaat ggcagcgcga gccgatgccg
     5101 taaaaagcta tctgaaagaa gatgaagctt ttaaccgcta tctcagtgaa ttgtgggggg
     5161 atttacggga atggctgaat gcggatatca acagtgaaga ttctcgtgtg aaagaacgta
     5221 tcgcgcgggc gggtcaatgg tttggcgaaa cgttaattgc cgatgatgcc ttgcgggcgt
     5281 cgttaaatgg tcatctggaa caagccgcac accgcattgc gcctgagttt tccgcattcc
     5341 tgactcgcca tatcagcgac acggtaaaaa gctgggacgc acgggatatg tcgcggcaaa
     5401 tcgagttaaa tatcggcaaa gatctccagt ttatccgtgt caacggtacg ctggttggcg
     5461 gttgtattgg acttattttg tatttgctgt cgcagctccc ggccttgttc cccctcggca
     5521 atttatagaa atccatgaaa aactaagtgg ctaaccgcga gagagatcaa ataaaccaca
     5581 ctaaaagaat ggtaataatg tggcctcttt tcattatctc ccgtggtacg gggaaggaaa
     5641 atcatgtcac ttgtcaccga tctacccgcc attttcgatc agttctctga agctcgccag
     5701 aaaggctttc tcaccgtcat ggatctcaag gagcgcggca ttccgctggt tggcacctac
     5761 tgcaccttta tgccgcaaga gatcccgatg gcagccggtg cggttgtggt ttcgctatgt
     5821 tccacctctg atgaaaccat tgaagaagcc gagaaagatc tgccgcgcaa cctctgcccg
     5881 ctgattaaaa gcagctacgg cttcggcaaa accgataaat gcccctactt ctacttttct
     5941 gatctggtgg tcggtgaaac cacctgcgac ggcaaaaaga aaatgtatga atacatggca
     6001 gaatttaagc ccgttcatgt gatgcagttg ccaaacagcg ttaaggacga tgcctcgcgt
     6061 gcattatgga aagccgagat gctgcgcttg caaaaaacgg tagaagaacg ttttgggcac
     6121 gagattagcg aagatgctct gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca
     6181 ctggccaact tttatcatct tgggcagtta aatcctccgg cgcttagcgg cagcgacatt
     6241 ctgaaagtgg tttacggcgc aaccttccgg ttcgataaag aggcgttgat caatgaactg
     6301 gatgcaatga ccgcccgcgt tcgtcagcag tgggaagaag gccagcgact ggacccgcgt
     6361 ccgcgcattt taatcaccgg ctgcccgatt ggcggcgcag cagaaaaagt ggtgcgcgcg
     6421 attgaagaga atggcggctg ggttgtcggt tatgaaaact gcaccggggc gaaagcgacc
     6481 gagcaatgcg tggcagaaac gggcgatgtc tacgacgcgc tggcggataa atatctggcg
     6541 attggctgct cctgtgtttc gccgaacgat cagcgcctga aaatgctcag ccagatggtg
     6601 gaggaatatc aggtcgatgg cgtagttgat gtgattttgc aggcgtgcca tacctacgcg
     6661 gtggaatcgc tggcgattaa acgtcatgtg cgccagcagc acaacattcc ttatatcgct
     6721 attgaaacag actactccac ctcggatgtc gggcagctca gtacccgtgt cgcggccttt
     6781 attgagatgc tgtaaggagt ggcagtggca tattcgattg gcattgattc cggctcaacc
     6841 gccaccaaag ggatcttact ggcagacggc gtgattacgc gccgtttcct cgttccaacc
     6901 ccctttcgcc cggcaacagc aattactgaa gcctgggaaa ctctgcgcga agggttagag
     6961 acaacgccgt ttctgacgct caccggctac gggcggcaac tggtggattt tgccgataaa
     7021 caggtaacgg aaatctcctg tcacgggctg ggcgcacggt ttcttgcgcc agcaacgcgc
     7081 gcggtaatcg acatcggtgg tcaggacagc aaagtgattc agcttgatga tgacggtaac
     7141 ctgtgcgatt tcctgatgaa tgacaaatgc gcggcgggca ccgggcgttt cctggaggtg
     7201 atctcgcgca cgcttggcac cagcgtcgag caactcgaca gcattaccga aaatgtcacg
     7261 ccgcacgcca tcacgagtat gtgcacagtg tttgctgaat cagaagcgat cagcctgcgc
     7321 tcagcgggcg tcgcgccaga agcgattctc gccggagtga ttaacgcgat ggcgcggagg
     7381 agtgccaatt tcattgctcg tctctcctgt gaagcgccga ttctgtttac tggtggcgtt
     7441 agtcattgcc agaagtttgc ccggatgctg gaatctcacc tgcgaatgcc ggtaaatacc
     7501 catcctgatg cgcaatttgc tggcgcaatt ggcgcggcgg taattggtca acgagtgagg
     7561 acacgccgat gaaagagttt ttatttttat ttcactccac ggtcggcgtc atacaaaccc
     7621 gcaaagcgtt gcaggcagcg ggcatgactt ttcgcgtcag cgatattccc cgtgatttac
     7681 gcggcggctg cgggttatgc atttggctga cctgtccccc cggcgaggaa atacaatggg
     7741 tgatccctgg gctgactgag tcaatttatt gccagcagga tggtgtctgg cgctgcattg
     7801 cacattacgg ggtttctccg cgttaaggat tcattaaggt cgctacgtat acttctcgat
     7861 atcttattga tttgccttaa agggattacc gtgacaaaaa gtatttcgtt aagtaaacgc
     7921 atctccgtta ttgtgattct ttttgccatt gtggcagtgt gtactttctt cgtgcaatct
     7981 tgcgcccgta aaagcaatca tgcagcatca tttcagaatt accacgcacc attgacggca
     8041 aagaaattgc aggcataacg aataatatct cttcgctgac ctggtcggca caaagcaata
     8101 ctctttttag cactatcaac aaacctgccg ccattgttga aatgaccacc aatggtgatt
     8161 ttatccgcac aattcctctg gattttgtta aggatctcga gacgatcgaa tacattggcg
     8221 ataatcagtt tgtcattagc gatgagcgcg attacgccat ttatgtgatt tcactgaccc
     8281 caaactcgga agtaaaaatc ctcaaaaaaa taaaaatccc gctgcaagac tccccaacca
     8341 attgcggttt tgaagggttg gcttattctc gccaggatca cactttctgg ttttttaaag
     8401 agaaaaatcc gattgaggtc tacaaagtta acgggctgtt aagtagcaat gaattacaca
     8461 tcagcaaaga cgaggcacta caacggcaat ttactttaga tgatgtgtcc ggcgcagaat
     8521 ttaaccagca aaaaaatacg cttctggtgc tgtctcatga atctcgcgca ctccaggaag
     8581 taaccctggt tggcgaggtt atcggtgaaa tgtcactaac caaaggcagt cgaggtttgt
     8641 cgcataacat caaacaggcc gaaggggttg cgatggatgc ctctggtaat atttatatcg
     8701 ttagcgaacc aaatcgtttt tatcgcttta cccctcaatc atcgcattaa caggctggca
     8761 tgcttcacct ttttgttatt aacttgttaa caacaaaaat gacaaacaag gttaaacatg
     8821 ccttcgtcca cgcatcccgt agaacgcttt tctttcagca ccgcgctttt cgggatgctg
     8881 gttctgacct taggtatggg tttaggccgc ttcctttata cgcctatgtt gcccgtcatg
     8941 atggcggaag gatcgttttc atttagccag ctctcgtgga ttgccagcgg caactatgcg
     9001 gggtatctgg ctggcagtct gctattttct tttggcgcat ttcaccagcc atcgcgcctg
     9061 cgcccatttc tgttggcttc tgccctggcg agcgggttgt tgatcctcgc aatggcatgg
     9121 ttgccgccat ttattctggt gttattgatt cgcgtcctgg cgggtgtcgc cagcgccggt
     9181 atgctgattt ttggttcgac gctgattatg cagcacacgc gccatccttt tgtgctggca
     9241 gctttgtttt ttggcgttgg cattggcatc gcactgggca atgaatatgt tctggcaggc
     9301 ctgcattttg acctctcctc gcaaacgtta tggcaaggcg ccggggcgct ttctggcatg
     9361 atgctgattg cacttacgct tttaatgccc tcgaaaaaac acgccatcac accaatgcca
     9421 ttggcaaaaa cggagcaaca gataatgagc tggtggttac tggctattct gtacggcctg
     9481 gcgggatttg gttatatcat cgtcgcgact tacctgccgc tcatggcgaa agatgcaggc
     9541 tcaccattgt taaccgccca tctctggacg ttagtcggct tatcaattgt gcctggttgc
     9601 tttggctggc tatgggcagc aaaacgttgg ggggctttgc cctgcctgac ggcgaatttg
     9661 ctggtgcagg ctatctgtgt gctgcttact ctcgccagcg actcgcctct cttgcttatc
     9721 atcagcagtc ttggttttgg cggtaccttt atgggcacga catctctggt gatgactatc
     9781 gcccgccaac tgagcgtacc gggaaatctc aatcttttag gctttgtgac gctcatttac
     9841 ggcattgggc aaatcctcgg cccggcgctg accagtatgc tcagcaatgg aacatcggcg
     9901 cttgccagcg ccactctctg cggcgcggcg gcgttgttta ttgctgcgct tatcagcaca
     9961 gtgcaattat ttaagctaca agtggtcact tcatgaggga agtttccctt accactcaag
    10021 aaactcaacc gcaaccgttg atgtcagcca gaccccgttt tccgcttgcc agaaagggag
    10081 gcctcgtttc gccatttcct gtgctttgac ggttaaaata accggcgatc cgtgtcttgc
    10141 tcccactttg cgtgccgttg cttcatcagc agagagatgt acgtagtgac gctcacctgc
    10201 aatcagcccc tgttttttta tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa
    10261 ctgtggcggt gttttttcgg caaaggaaat agcgacctgc gaagtcgaat gcccctgaac
    10321 cgcgcggatg cacctaccat cactggaata actaaaacgc tttttatcgc tggtggcaac
    10381 cacagtatcc agcaatgcac gggttaaccg cttgcccgct ttttgggcac aaagtatgag
    10441 cttatcgata tccgcccatc cttcacggtc cagtacgata ccaatcgcct ccggtttgtg
    10501 tcggaggaca aaacttaaaa acttgctcgt atccgccagc tctttttcgt tgtactttgc
    10561 catctcgttt tcgtccctgg attttctgtt ttgatttact gatgtcattg tcacttggtt
    10621 tatgaatttt ctgcaactca atcctgcgca gagccttttc cacacagcgt aatttttccg
    10681 ctatgcaaaa aacgcatagc cagaaacata cttgcatttt ccccataccc acatggctga
    10741 taaggttttt cagttccagg aattttcctg gctcaagcaa cacacaaaca caaaaaaaca
    10801 atggggattg ttatgacaca acaaggggat gctgtagccg gggagctggc gacagaaaaa
    10861 gtgggcatta aagggtatct ggcctttttt ctcaccataa tctttttttc cggtgttttt
    10921 tccggaacag acagctggtg gcgcgttttt gatttttccg ttctgaacgg atcgtttggg
    10981 caactgcctg gtgcgaacgg agccaccacc tcatttcgcg gcgcgggtgg cgcgggggct
    11041 aaagatggct ttctctttgc gctggagctg gcaccgtcag tcattctctc tctggggatt
    11101 atttcaatta ccgatggtct tggtggatta cgcgccgcgc agcagttaat gacacctgtg
    11161 ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg cgctgatcgc taacctgcaa
    11221 aacaccgatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga aattaccgaa
    11281 cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat caccaactac
    11341 ttctcttccg gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc
    11401 gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct taacttcgaa
    11461 gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca
    11521 tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc aacctgctgc
    11581 caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc ctgctcgact
    11641 gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attacccggt gaagcggcaa
    11701 ccgtcctttt agccgcatta atgagtatgg gtggcgcagt cggtgttgcc gccagtctgg
    11761 caaccgctgg cgcattaacc ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa
    11821 tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg aatgccaaat
    11881 attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc
    11941 agcttattgt ttaacaagga gttaccatga ttgattatac cgcagccggt tttaccctgc
    12001 tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta
    12061 acggcaaaat tatcgccgtt gccagcaata tcccttctga cattgtaccg aactgcacgg
    12121 ttgtcgatct cagtgggcag atcctctgcc caggttttat tgatcaacac gtccatttga
    12181 ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc
    12241 tgacggaagc gggcgtcacg tcagtggttg gtctgctggg caccgactct atctctcgcc
    12301 acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc agcgcctgga
    12361 tgctgaccgg cgcttatcat gtcccttccc gcaccattac gggttccgtg gaaaaagacg
    12421 tggcgattat cgatcgtgtg attggcgtga aatgcgccat ctctgatcac cgttctgccg
    12481 caccggacgt ttatcacctg gccaatatgg cggcagaatc ccgcgttggc ggtttgctcg
    12541 gcggtaaacc tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg ttacagccta
    12601 tttatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta
    12661 accgcaacgt accgttgttt gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg
    12721 atatcaccag cagcattgac gaaccggtcg cccctgccga aggtattgcc cgcgccgttc
    12781 aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt agccagccgt
    12841 tcttcgatga cgaagggaat ttaacccata tcggtgttgc cggttttgaa acgttgctgg
    12901 aaaccgtgca ggtgctggtc aaagactatg atttcagtat cagcgatgcc ctgcgcccgc
    12961 tcaccagtag cgtagccggt ttccttaacc tgaccgggaa aggcgaaatt ctgccaggca
    13021 atgatgctga cttgctggtc atgacgccag aactgcgcat tgagcaggta tacgctcgcg
    13081 gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa
    13141 cggttgtcag ccgcccgaat ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac
    13201 gtaatggatg actgtggtgc gattttgcat aatattgaaa ccaaatggct ttatgatttt
    13261 ctgaccctgg aaaaatgccg caatttttcc caggcggcag tcagtcgcaa cgtctcgcaa
    13321 ccggcattca gccgccgcat ccgtgcgctg gaacaggcga ttggtgttga attgtttaac
    13381 cgccaggtaa cgccgctgca actctcagaa caagggaaaa tcttccattc gcagatccgc
    13441 catctgttgc aacagttaga gagcaatctg gcagagctgc gtggcggcag cgattacgcg
    13501 caacgaaaaa tcaagatagc cgctgcacac tctctttccc tcgggctgtt accgtccatt
    13561 atcagccaga tgccaccgct ctttacctgg gcaattgaag ctattgatgt cgatgaagcg
    13621 gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga cgaagatttg
    13681 ctggaagcgc cgtttgacca cattcgctta tttgaatctc aattgttccc tgtctgcgcc
    13741 agtgacgaac acggagaagc actttttaac ctcgcgcagc cacactttcc gttactgaat
    13801 tacagccgca actcctacat ggggcgattg attaatcgca ccctgacgcg ccacagtgag
    13861 ttaagtttca gcaccttttt tgtctcttcg atgagcgagc ttttaaagca ggttgccctc
    13921 gacggctgtg ggattgcctg gctgccggag tacgccatcc aacaagaaat tcgcagcggg
    13981 aagctcgttg tactgaatcg ggacgaactg gtgatcccga ttcaggccta tgcataccgg
    14041 atgaataccc gcatgaatcc cgttgccgaa cgcttctggc gtgaactgcg cgagctggag
    14101 attgtgctta gctgacattc tccagcgtcg cactgcgcca accgccatta tcccagacca
    14161 gatcaaacgt tagcggttca tcgtcgtggt acagcattat ctcaaggctg atcgccagag
    14221 tgtggcagta cgcttctttc aaattaacct gcaacgccgc aatccgtggc tcatgaaata
    14281 acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg ctcgggttat
    14341 gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat
    14401 taaaaagagt gaggagatcg ctgcataccg atttacgcag cgatgacgtt ttattgcctg
    14461 aaatttcagg taaaacagcc tgaagtgatt gtcgcatcat tttgcgcact cctgacgttt
    14521 agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct cattttccat
    14581 agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga atcctgtcag
    14641 caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa aaaagtcatg
    14701 atttgataaa aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa tattccgaat
    14761 agttacgtag atgaaaatac ttgtcaccgc tgctacttaa ttccgg
//
LOCUS       DAZFRG010000112        14563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFRG010000112 DAZFRG010000000
VERSION     DAZFRG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.112"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..168
                     /locus_tag="ACHKUC_002782"
     CDS             <1..168
                     /locus_tag="ACHKUC_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6619014.1"
                     /translation="QVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKISASQS
                     AGSKPEEPVTP"
     gene            194..466
                     /locus_tag="ACHKUC_002783"
     CDS             194..466
                     /locus_tag="ACHKUC_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6619015.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            543..758
                     /locus_tag="ACHKUC_002784"
     CDS             543..758
                     /locus_tag="ACHKUC_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6619016.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            763..1296
                     /locus_tag="ACHKUC_002785"
     CDS             763..1296
                     /locus_tag="ACHKUC_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6619017.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPSKCFTSTFYRKLNAGDRKGACAEIRRWIYDGGRDCRNRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1453..1635
                     /locus_tag="ACHKUC_002786"
     CDS             1453..1635
                     /locus_tag="ACHKUC_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619018.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1650..1781
                     /locus_tag="ACHKUC_002787"
     CDS             1650..1781
                     /locus_tag="ACHKUC_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619019.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFFSTAWRVLTLSGLVG"
     gene            1789..2256
                     /locus_tag="ACHKUC_002788"
     CDS             1789..2256
                     /locus_tag="ACHKUC_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6619020.1"
                     /translation="MSRVLCVVIIVLAGGCGALWLATDHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGMDNATGPRLADTAERDYFTLRERLMTMQKQLEGAQEYISTQCLK"
     gene            2558..3409
                     /locus_tag="ACHKUC_002789"
     CDS             2558..3409
                     /locus_tag="ACHKUC_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6619021.1"
                     /translation="MAKLDWKKLEQAFRREHARTGIKLQDWCRQNNICYGTARRYIKL
                     RKNSPKNTEESAQKNAQKSAQIDTQKSAQKNAHGADDESSNDESYGDDGSDEKCAKNC
                     ANSAETKRNSRSGNPHPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVF
                     TRARALSVTGTLKKMFADLKEAADVETRVALYDKILKAEQALDRNIARIESIERSLLT
                     LDVLAETAPKLRADRERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGR
                     LDDIPEE"
     gene            3390..5096
                     /locus_tag="ACHKUC_002790"
     CDS             3390..5096
                     /locus_tag="ACHKUC_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6619022.1"
                     /translation="MTFRKNEPRCDEPSEMTGTEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLALTPQDYKFHFYAWWQDLKYSARVPESGLKLSREKTAYFSAVEKAMN
                     ITLTDEQKHWYICKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGTKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGLSGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            5096..7240
                     /locus_tag="ACHKUC_002791"
     CDS             5096..7240
                     /locus_tag="ACHKUC_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6619023.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPLEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLLVISDDPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMHAVAVASGRVQVRVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNELMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSNASSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEAAAQQQALEQ
                     QQAELQMREMTGRVAKLEAEAARARAAAQRDNAGAQRDVAAAQGQRYVDALNQAHTAE
                     IITGIQNMEQEQEVLQQQMLHTLQQRMHEMPL"
     gene            7398..8405
                     /locus_tag="ACHKUC_002792"
     CDS             7398..8405
                     /locus_tag="ACHKUC_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619024.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDGHEQDGVTEAATENHAD
                     EAAQTQTGDNTAAPTPDASAEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRLVELLTSQIHQAGMKPAPLPENEKISDEKIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGHGGEDLSPVLDAMNAVPVLKTWQNSDPDRFSVA
                     VSIDGKLQNDPAWKDKPLTERFAEVARRTQLAFGEASEPPSDDKADNTDIRKTAEEKV
                     KAAEREMAVPASPSELGNTASVGTGDNFERLLGASHTEIEAAMRGMTDAEIDAILEKL
                     G"
     gene            8428..9642
                     /locus_tag="ACHKUC_002793"
     CDS             8428..9642
                     /locus_tag="ACHKUC_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO6619025.1"
                     /translation="MTTVTTAQANKLYQVALFTAANRNRSMVNILTEQQDAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTADHPEFKKIMINDVLPPTYDRHFFGGDATSFDGIDQSDLFTLGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMTRAVNRS
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSKNDLTATTEEKQAATNIDR
                     AMLLGAQALANAYGQKGGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGRMQD
                     HGVIAVDTAVKL"
     gene            9697..10089
                     /locus_tag="ACHKUC_002794"
     CDS             9697..10089
                     /locus_tag="ACHKUC_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619026.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLFS
                     LPIGVRINALQLVSTKGLGGTATVSVKSGEHELIGDSGAVTASFTKYVPVEPYTTQRD
                     GELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            10140..10601
                     /locus_tag="ACHKUC_002795"
     CDS             10140..10601
                     /locus_tag="ACHKUC_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619027.1"
                     /translation="MSEKIAVVYIGPKPVKKDTLTGSRTLFPRLEPVHVDSALAWQLL
                     AFPDVWVRHEELDGVLKKQQQDEQLRQAQQAQEREQVALAEAENSFVVSVGGQDVDLS
                     KLTSARLATLCEAEELDIHKDPKETADAFRVRVREAFRRRVAETEQHGGTD"
     gene            10585..11148
                     /locus_tag="ACHKUC_002796"
     CDS             10585..11148
                     /locus_tag="ACHKUC_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619028.1"
                     /translation="MAELTDFLPYVRRHISGPLNIMMTDALSMSAVAFCRQSLLCRRE
                     ATLSPSAGEDCVLPYDAENEECVHIIRILADGRELFAGHDVEIRPGRVMHFACSPGEV
                     SVLYAVAPKAGSRQVQDELRAWPEEVAAGALERLFMQTGVSWSDPSRAQYFSVLFSEG
                     IRRAYRDTLATSPYSSYRNPVRRQRFY"
     gene            11148..11798
                     /locus_tag="ACHKUC_002797"
     CDS             11148..11798
                     /locus_tag="ACHKUC_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO6619029.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLNCVPGARQTLPDGAIQLLDVICLSDGSAVKPQSREVLDAQYPDWHMLRG
                     KPECFISSDLAPRVFWLFPAPEEAVSVDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGM"
     gene            11795..13783
                     /locus_tag="ACHKUC_002798"
     CDS             11795..13783
                     /locus_tag="ACHKUC_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6619030.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     DGGYYGMTLEPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTAVKNNQASQSKESAAASAKTASESAKAATSKAT
                     ESAQKASEAAEAATRAGESEKAAAADAKEARRHAETARVAQEAAGDVLKRAEAATVSA
                     EEARRMAENARGPKGDTGPKGDAGPRGETGPAGPRGEKGEQGERGPQGIPGQKGDTGE
                     RGPQGVQGPAGATGPSGPRGATGPQGAMGPRGETGARGEKGDPGGPQGPKGDAGPRGE
                     PGPKGDTGPRGEAGPPGPQGPAGQTGPKGDKGEPGATGPAGPAGPRGETGPAGPAGPA
                     GSVASVPDASTSQKGVVQLSSDTNSDDETKAATPKAVKAVMAEVQAAKTKAEEAATRA
                     AVPGPKGDRGEPGAPGAVGPAGPRGPAGAAGPKGDAGPAGKDGTAGAEGKAGPAGPRG
                     ERGPAGAQGVPGPVGPAGPAGKTGPQGPAGGRGPMGLPGLDGKTGPQGPQGPQGPQGP
                     TGRLVPGEIYSIGTYIIAALGPYITDIGRTYQPGETVSGSRLKRCALIKDENGNYMKA
                     NTDGIDGNLSSVPGSWMVCNEITSTNDNEGIGLFQRAY"
     gene            13803..14381
                     /locus_tag="ACHKUC_002799"
     CDS             13803..14381
                     /locus_tag="ACHKUC_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6619031.1"
                     /translation="MWKKISNPQWADKDHTAVNCMVKFEHIEQAVPFTATASDTEAYG
                     RDIYAACLRGEAGEIAEYVQPSISPEKARELKTAGINAWRNAMEAANYTFEHNGRKWD
                     YGKQTQARLEPSVAAAKAGILPEGFFWTDAENNDVPMTAEALMALSEAAEKAMFTKGM
                     EIHVRQRTMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT         3716 a         3457 c         4342 g         3048 t
ORIGIN      
        1 caggtgatgc tgtacttctg tacggcggtc tgtgtgctgt atcttctttc gggtgggtac
       61 agggctgtgc gcgatttctg gtgcaggcag attgataaaa gggccgctga gaaaatcagc
      121 gccagtcagt cagccggaag caaacccgaa gagcccgtta ctccttaaca acccctttca
      181 gcgagaaaat cccatgtcag aaatcacatc cctggtcact gcagaggcag tgaaggaagt
      241 cctgcgctct gaagaagtcc ggagcgcact gaaacagaaa cttcgccaga atcttgaggc
      301 gcgtcttgat gcagaagttg atgccattct ggatgagctg cttggtgtac aggcagagcc
      361 accgactgaa gcgggagata ccaccgcaga gagcggtgaa gttcagcctg aatcaccggt
      421 cgccgatgcg actgaacctc aacccgaatc ggtcatgatg ctgtaacgag gagtcagggc
      481 catcagtaaa cggctgctgg cctttttcat gttgtgagct tccggattgc gggagacggg
      541 gtatgtacca gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa
      601 cgggctactg gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag
      661 gcgtgctggg gagtctgctg tttgggctgc tgacatatct gacgaacctg tatttcaaaa
      721 ttaaagaaga ccggcgtaag gcggcgcggg gagagtaagc tgatgagcag gaaattccgc
      781 tatggtttat cagcggtcgt tctggcgatg attgctgcag gtgcttctgc gcctgaaatc
      841 ctcgaccagt ttcttgacga aaaagaaggt aatcgcacca cagcataccg tgatggtgcg
      901 ggtatctgga ccatctgccg tggtgccacc cgggtggatg gtaagcccgt cgttcccggc
      961 atgaagctgt cgaaggaaaa atgcgcacag gttaatgcca ttgagcgtga taaggcgctg
     1021 gcatgggtgg agaaaaacat cagagtgcca ctgaccgaac cccagaaagc ggggatcgcg
     1081 tcattctgtc cgtacaacat cgggccttcg aaatgcttca cctcaacgtt ttaccggaaa
     1141 ctgaacgccg gagaccggaa aggtgcatgt gctgaaatcc gccgatggat atatgacggc
     1201 ggcagggact gccggaatcg ctcaaataac tgttacgggc aggtatcgcg gcgtgaccag
     1261 gagagcgcgc tggcgtgctg gggaattgac agataagcag aatattttgc tgaaaaatgc
     1321 ggtttgctca cacggacgga taacacgaaa tcctgcgaac tgacaaaaac taagtgaata
     1381 aaaataaaaa ccccgtttgt tggctgcaag cggggttttg tgtttctgac cttggataag
     1441 gcaagggaga acatggaaaa gtataaacga attctgttga ggttgactat gaaaaacggc
     1501 cttgaactga aagcgcctgt aactgatgac atcagcagag cactggcttt tgccattaag
     1561 tgggtggcgg tcggtgttgc tgtgtccccg atgctgtacg ggctggcaaa actggtcatt
     1621 gcgttgaaat cgtgaaggga ggattaagca tgtcagacaa actcataacg ctggcgaaga
     1681 tcctctgtgt aattgtcggc atttcatttt cactcatgct ggttgccatt ttcttttcca
     1741 ccgcctggcg agtcctgacg ttatcgggac tggtggggtg aaagagagat gagccgtgtt
     1801 ctgtgtgtgg tgattattgt cctggcgggt ggctgtggtg cgctgtggct ggcaacagac
     1861 cattaccgcg ataacgccat cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg
     1921 gagctggcaa acgccaccat tactgatatg caggtgcgcc agcgcgatgt tgctgcgctc
     1981 gatgcaaaat actcgaggga attagccgat gcgagagctg aaaatgaaac tctgcgtgct
     2041 gatgttgctg ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt
     2101 gaagccaccg gcacctccgg catggataat gcaaccggcc cccgactggc agacaccgct
     2161 gaacgggatt atttcaccct cagagagcgg ttgatgacga tgcagaagca gctggaaggg
     2221 gcacaggaat atatcagcac tcagtgcctg aaataagttt tgctgatgcg ccgtatcgtc
     2281 gcagtattcc cgcattaaca gagacctcag ccagacactg gacctcctgc gcgagtgtgt
     2341 ggggataatc aaaaacgatg cacaccgggc tttccacgtt ttcaggtaac gtgagcgttt
     2401 tcccgcatgg ccgcctgtcc ggtgcggtgg tggaagaaac cggataaaac aaccatattg
     2461 tgtaaatatc aatcaaacaa gacgctgctg tgtgaattct gaaaaatcac agcggtcatt
     2521 atgcgtcagt ttttaacaga ggacgtcaga aagtgacatg gcaaagctgg actggaaaaa
     2581 actggagcag gcattccgac gcgaacatgc cagaactggc ataaaacttc aggactggtg
     2641 tcgacagaac aatatctgct atggcacggc tcgtcggtac ataaaactgc gcaaaaactc
     2701 ccccaaaaat acagaggaaa gtgcgcagaa aaatgcgcaa aaaagtgcgc aaattgacac
     2761 tcaaaaaagc gcacaaaaaa atgcgcatgg agctgatgac gagtccagta atgacgagtc
     2821 ttacggcgat gatggcagtg atgaaaaatg cgcaaaaaac tgcgcaaatt cggcagaaac
     2881 gaaacgcaac agcagatcgg gtaatcctca ccccgttgcc cggtttagcg accgtaacac
     2941 ccacgccgtg agacaccgtg gatatgcgaa gtatcttgag gcagataacc tcatggatga
     3001 tgcgtccgat atggtgctgt tcgatgaact ggtgttcacc cgggcccgcg cactttcagt
     3061 aacaggaaca ctgaaaaaaa tgttcgccga cctgaaagag gcggctgacg tggaaacccg
     3121 tgttgccctg tacgacaaaa tactcaaagc agaacaggcc cttgaccgga atattgcccg
     3181 tatcgagtca attgaacgct cattgctgac gctggacgtt ctggcggaaa cagcaccaaa
     3241 acttcgtgct gaccgggaaa gaatcaacgc cgccagagat aaactcagag cggaaaccga
     3301 tattctgacc agccagcgcc ggggggttat tacgccagtc agtgacatcg tgtcatcgct
     3361 gcatgaaatg agtaattcgg ggagactgga tgacattccg gaagaatgaa ccgcgatgtg
     3421 atgaaccgtc agaaatgacc gggaccgaac aacgtctgtt catcatgact aaactgagca
     3481 atccctggtg gcggctcaat catctctaca aaatacagaa cgaaaaaggt gagctggtca
     3541 ccttcagaat gcgaccggcg cagcgccagt tgttccggag catgcacaat aaaaatatta
     3601 tcctgaaagc acgccagctg gggttttcca cggccattga tatttatctt ctcgaccagg
     3661 cattattcat accgcatctc aaatgcggga ttgtcgctca ggataaacag gctgccagtg
     3721 aaattttccg cacaaaaatt gctgtaccgt ttgatcatct ccctgactgg ctgagagcct
     3781 cattcaccat cgttgaacgt cgtagcggag ccagcggtgg ctatatcctg tttggtcacg
     3841 gctcgagtat ccaggtggca acctcattcc gttcaggtac ggtgcagcgc ctgcatatct
     3901 cagagcacgg caaaatttgc gcaaaatatc cggctaaggc gaaagaactg cgaaccggta
     3961 cacttaatgc cgtctctgat gagtgcatta tctttgatga atccactgct gaaggtgtgg
     4021 gtggcgattt ttacgagatg agtaaccggg cacaggaaat cactgcatca gggctggcgc
     4081 tgacgccaca ggattataaa ttccattttt acgcctggtg gcaggatctc aaatacagcg
     4141 ccagagtgcc ggaaagcgga cttaagctgt cacgggaaaa aacagcgtat ttttctgcgg
     4201 ttgaaaaagc aatgaacatc acgcttaccg atgagcagaa acactggtac atctgcaagg
     4261 aaactgaaca gcgtgaggaa atgaagcagg aatttccctc aacgccacag gaggcgtttc
     4321 tgacgtcagg acgacgggtg ttcagtgcgg aaagcacgct gcaggcagag tcattctgtt
     4381 cgccaccgct gattgtttat gacattgaac cggttacagg aacgaagacc aaggcgcagt
     4441 ctctgcgtga cgggaataaa gccgaacagc accggacgct gatgaattat ctgctggtct
     4501 gggagctgcc ggatccggat gaagagtatg tatgcggagc agataccgct gaggggctgg
     4561 agcacggaga ccgctcatcg ctggatatca tcagatgcag taatggtgag caggtggctc
     4621 actggtttgg tcatctcgat gcggagcttt tcgctcatct cattgcgcag gtctgccgta
     4681 tgtacaacaa cgcgtttgtg gggccggagc gtaacaatca cggacatgcc gttatcctga
     4741 aactcaggga actctatccg acgcgttata tctacaacga acagcacctt gaccaggcat
     4801 atgacgacga tacgccccgt cttggctggc tgacaacccg ccagagtaaa ccggtcctga
     4861 cagagggaat gaaaacgctg ctgaataacg gcctgtcggg gatccgctgg tcgggcacat
     4921 tatcagaaat gaacacctac gtttatgacg cgaaaggctc catgaatgca caggaaggct
     4981 gttttgatga tcagcttatg agctacatga ttgcccagga gatgcgcgcc agaatgccgg
     5041 tgagggtaaa acagaaaacg gataaacgca gaaccacaca ctggatggct cactgatgaa
     5101 aaatgaaact aataccatgg cgacgaaaaa cgacaacgga gccacgccgc gtttttctca
     5161 gcgccagtta caggcgcttt gttctgatat tgacagtcag cctaaatggc gcgatgccgc
     5221 aaacaaggcc tgtgcgtatt acgatggtga ccagttgcca ctggaagttc tccaggtgtt
     5281 gaaagatcgc ggtcagccga tgaccatcca taacctcatc gcgcctaccg ttgatggtgt
     5341 tctgggtatg gaggccaaaa cgcggactga tctgttggtg atatcagatg atcctgatga
     5401 tgaaacagaa aaactggctg aagctattaa tgctgaattt gccgatgctt gccgccttgg
     5461 aaatatgaat aaagcccgtt ctgatgccta tgcggaacaa atcaaagcgg ggctcagctg
     5521 ggtggaggtc agacggaaca gcgatccgtt cgggcctgag tttaaggtgt ctactgtcag
     5581 ccggaatgaa gtgttctggg actggctcag ccgggaggct gatttaagtg actgccgctg
     5641 gctgatgcgc cgccgctgga tggataccga tgaggcaaaa gccacatttc cgggaatggc
     5701 tcaggttatc gattatgcca ttgatgactg gcgtggtttt gtcgatacca cggttactga
     5761 aggccagccc agtccgttga tgagtgcatg ggaagagtat cagtcatggg atcgacagca
     5821 gaacgaatgg cttcagcgtg aacgccgtcg tgtgctgctt caggtggttt attaccgtac
     5881 attcgagcgt cttccggtga ttgaactcag taatggacgg gtggtggcct ttgataaaaa
     5941 taatctgatg catgcggtgg ctgtggcatc cgggcgggtg caggtgagag tcgggcgggt
     6001 aagtcgtatt cgtgaagcct ggtttgtggg accgcacttt attgtggatc gcccctgcag
     6061 tgcgccgcag gggatgttcc cgctggttcc cttctgggga taccggaagg ataaaaccgg
     6121 ggagccttac gggctgattt cccgggccat tccggcacag gatgaggtga attttcgtcg
     6181 tataaaactg acgtggttgc ttcaggccaa acgcgtgatt atggacgagg atgccaccca
     6241 gttgtcagac aacgagctga tggagcagat cgagcgtccg gatggcatta ttaaactgaa
     6301 tccggcccga aaaaatcaga aaagcgttgc tgatgttttc cgggttgaac aggattttca
     6361 ggttgccagc cagcagtttc aggtcatgca ggagtcggaa aaacttatcc aggacacgat
     6421 gggggtttat tccgcatttc tcgggcagga ttcaaatgca tcgtccggtg tggcgatcag
     6481 caacctggtg gagcagggag ccacaaccct tgccgaaatc aacgataact accagtttgc
     6541 ctgccagcag gtgggaaggc tgttgctcgc ttatcttctt gatgacctga aaaagcggcg
     6601 caatcatgcg gtggtgatta atcgcgatga tcgtcagcgt cgccagacca ttgtcctcaa
     6661 tgctgaaggt gataatggtg aactgaccaa tgatatttca aggttaaata cacatattgc
     6721 gctggctcct gttcagcaga caccggcgtt taaggcacag cttgcacaga gaatgtcaga
     6781 ggttattcag gggctgccgc ctcaggtgca ggctgttgtg ctcgatctgt gggttaatct
     6841 tctggatgtg ccgcagaaac aggaatttgt cgaacgtatt cgtgcggcgc tggggacgcc
     6901 aaaatcaccg gatgagatga ctccggaaga gcaggaagcg gcagcacaac agcaggcgct
     6961 ggagcaacag caggcagaac ttcagatgcg cgaaatgaca ggccgggtgg ctaaactgga
     7021 agcagaagct gccagggcac gcgcggctgc gcaacgggat aatgctggcg cacaacggga
     7081 tgtcgccgcg gcacagggac agcgttatgt ggatgcgctt aaccaggcac atacggcaga
     7141 aatcattacc ggcatacaga atatggaaca ggagcaggaa gttcttcagc aacagatgct
     7201 gcatacacta caacagcgga tgcatgaaat gccgctctga aaatcctgaa cttaacggaa
     7261 ccccgtcatc gtacggggtt ttttgtttcc ggagataagc gttccgggag cggtgcgctt
     7321 attcgcgggg gcagcgataa gccttattta ctcaaccatt cggatctgtc cgataaacag
     7381 accatgcgga gttatttatg gattttgaat ttacgggtga agaaaccccg gaacaactgg
     7441 aaaaaatgct ggaagggctt ggggatgtgg atattgacgg tcacgaacag gacggcgtga
     7501 cagaggccgc cacagaaaat catgcggatg aggcagcaca gacacagacg ggcgataaca
     7561 ctgcggcacc gacgccggat gccagtgctg agcagacgca ggacgtgaag gagccggagg
     7621 cgaagggtgt gctcacccgc gatggtaaac acgtcattcc ctatgaagtc cttgaggctg
     7681 aacgttccgg taagcagcgg gccgaacagg aagccgcact tcttcgggga cagatagccg
     7741 aagaaaaacg cctggttgaa ctgctgacgt ctcagatcca ccaggccggt atgaaacccg
     7801 caccgttacc ggaaaacgaa aaaatctctg atgagaagat tgcccgtatc agggagatgt
     7861 atccggaaat tggtgacgcg gtggcttcgc tcatccgtaa aaataactat ctccagtccc
     7921 gtgttcagca atcagcacag caggcagaag gtcatggtgg tgaggattta tcaccggttc
     7981 ttgatgcgat gaatgccgtg ccggtgctga aaacatggca gaactccgat ccggatcgct
     8041 tctcggttgc cgtatccatc gacgggaagc tccagaatga ccccgcatgg aaagacaaac
     8101 cgctcactga acgtttcgcc gaagtggccc gccgtacgca gcttgctttc ggtgaagcca
     8161 gtgagccgcc ttctgatgac aaggcagaca acacggatat ccggaaaacg gcggaagaaa
     8221 aagtgaaagc cgctgaacgg gagatggcag tgcctgcttc cccgtcagag cttggcaaca
     8281 cggcttccgt cggaaccggt gataattttg aacggttact tggtgcttcc cataccgaaa
     8341 ttgaagcagc catgcgaggc atgacggacg ctgaaattga cgcgattctg gaaaaactcg
     8401 ggtaaccact gaaggagtac tgacgtaatg accacagtaa caacagccca ggcgaataag
     8461 ctgtatcagg tggcgctttt cacggctgcc aaccgcaacc gctcaatggt gaatatactc
     8521 actgaacagc aggatgcacc aaaagcagtt tcgcctgaca agaaaagcac gaagcagacc
     8581 agtgccggtg caccggttgt gcgaatcacg gacctcaaca aacaggccgg tgatgaagtg
     8641 acgttcagca tcatgcacaa actctctaaa cgtccgacga tgggggatga gcgcgttgaa
     8701 ggccgtggtg aagacctcag ccatgctgat ttttccctga aaattaatca gggccgtcac
     8761 ctggtggatg cgggcggacg tatgagccag cagcgtacga agttcaacct ggcatcctct
     8821 gccagaacgc tgctgggtac gtactttaac gacctgcagg accagtgtgc gatagtgcat
     8881 cttgccggtg cccgtggtga ttttgttgct gacgacacca ttctgccaac ggcagatcac
     8941 cctgaattca aaaaaatcat gatcaacgac gtactgccgc cgacgtatga ccgtcatttt
     9001 tttggtggtg atgcgacgag ttttgacggt attgatcagt cagacctgtt cacccttggg
     9061 ctggtcgata atctctcgct gtttattgac gagatggccc atccgttaca gccggtgcgt
     9121 ctgtccggag atgaactgca tggcgaagat ccgtattacg tcctgtacgt tacgccgcgt
     9181 cagtggaatg actggtacac ctccacgtcc ggcaaggact ggaaccagat gatgacgcgt
     9241 gccgtgaacc gttcaaaagg gtttaatcat ccgttgttca aaggcgaatg tgcgatgtgg
     9301 cgcaatatcc tggtccgcaa gtatgcgggg atgccgatcc gtttctatca ggggtcaaag
     9361 gttctggtat cgaaaaacga cctgacggca accacagaag agaagcaggc cgcaaccaac
     9421 attgaccgcg ccatgctgct gggtgctcag gcactggcga atgcgtacgg tcagaagggc
     9481 ggcggtcact tcaacatggt tgagaagaaa acggatatgg ataaccgtac tgagatagca
     9541 atcagctgga tcaacggtct gaaaaaaatc cgtttcccgg agaagagcgg caggatgcag
     9601 gatcacggtg tgattgctgt tgataccgca gtgaagctct gatttttcct ttcctcatgc
     9661 cgggttatcg cccggctttt tcaggagtca ttaattatgg caaagaccat ccttgccccg
     9721 tcactgagtg aacgggtcta tacgggtacg cacggtaatg agtcggtggc agaaggtgtg
     9781 tttactgtga atgctgcgga agcggacagt gttattcatc ttttttcgtt gccgatcggt
     9841 gttcgtatta atgcgttaca gctggtttct accaagggac ttggcggcac agcaactgtc
     9901 agcgttaaat ctggcgaaca tgaactcatt ggtgacagtg gtgcagtaac tgccagcttt
     9961 accaagtatg tgccagtgga gccgtacacc acacagcgtg acggagagct ggttacggtc
    10021 accattaaga cagcagctgc aaccggtacc ctgaatgttc tgctgcgtta taccgtggtg
    10081 ggatactgat ataaaccttc cggcccgcat catgcgggct tttttatccg gggaattata
    10141 tgagcgagaa aattgccgtt gtctatatcg gccccaaacc cgtgaaaaag gataccctta
    10201 ccggcagtcg tacactgttt ccgcgtcttg agccggtgca tgttgacagc gcgcttgcct
    10261 ggcagttgct ggcctttccg gatgtctggg ttcgtcatga agagcttgat ggtgtgctca
    10321 aaaagcaaca acaggatgag cagttgcggc aggcacagca ggcgcaggaa agagaacagg
    10381 tggctcttgc agaagcggag aacagttttg tggttagcgt tggtggtcag gatgtggatt
    10441 tgagcaaact cacgtccgca cggctggcaa cgttgtgtga ggcagaagag ctggatattc
    10501 acaaagaccc gaaagaaacg gctgatgcat ttcgtgtccg tgtgcgtgag gcatttcgcc
    10561 gtcgtgttgc ggagactgaa cagcatggcg gaactgactg attttttacc gtatgtccgt
    10621 cgccatatca gtggtccgct gaacattatg atgacggatg cgttatccat gtcggcagtg
    10681 gcattctgcc gtcagtccct gttgtgccgc cgtgaagcca cgctgtcacc gtcagccgga
    10741 gaggactgtg tgctgccata tgacgcggag aatgaagagt gcgtacatat tattcgtatc
    10801 ctcgctgacg ggcgtgagct ttttgccggt catgatgtgg aaatccgccc ggggcgggta
    10861 atgcattttg cctgttcgcc cggagaggtg agtgtgcttt atgccgttgc tccgaaagca
    10921 gggagccgtc aggtgcagga tgaactgcgg gcatggcctg aagaagtggc tgcaggggcg
    10981 cttgaacggt tgttcatgca gaccggggtt tcatggtcag acccgtcgcg cgcacagtat
    11041 ttctctgtgc tgttttctga gggtatccgt cgggcatatc gcgacacact ggcgacaagt
    11101 ccgtactcct cataccgcaa tcctgtccgc agacaaaggt tttactgatg acgacgatta
    11161 ctgaaatcat cggacgagtg aacacgcaac tggttgaccc gatgatggtg cgctggcctc
    11221 tggctgagct gtgtgattat tacaatgatg ctgtgcgggc ggtgattctg gcaaggccgg
    11281 atgcaggcgc aagtctggag acgctgaatt gtgttcctgg tgcacgtcag actttgcctg
    11341 atggcgcaat acagcttctt gacgtgatat gcctcagcga tggcagtgct gtaaaaccgc
    11401 aatcccgtga ggtgcttgat gcacagtatc ccgactggca catgctgagg gggaaaccgg
    11461 aatgctttat cagcagtgat cttgccccgc gcgtgttctg gctgtttccg gcaccggagg
    11521 aggctgtgag tgttgatgct gtggtcagcc gcattccgga ggcggtgtat gttctgacgc
    11581 aggatgatga cacgcctgta ccgctggaag aggcttatgt taacccgctg gttgactgga
    11641 tgttgtttcg tgcattcagc aaggatgcag ccggtggtgc agagtcagga ctggcagcac
    11701 aacattatca gagttttgtt gagcagctcg ggattaaaca gggtgcagac agtgcattgt
    11761 ctgcccgcaa gaaggtgttt aacggaggtg gaatgtgagt gttgttgttt cggggacgct
    11821 gaaatcgcct gatggtgagg cgatatcagg cgcaaatatt accctgacgg cgctgacagt
    11881 ttcaccggat gcgctcagcg gcaccagtgc gtcagcagtg actcgtgacg gcgggtatta
    11941 cggaatgacg ctggagccgg gggagtatgc ggtttctgtg acggtgaaag ggaagactgc
    12001 tgtctacgga cgtgtgcgta ttgaggggac tgaaagtacg gtgacgctca atatgctgtt
    12061 acgccgcagt cttgttgagg tgagcatacc cggagaactg ctgacagatt tccggcagat
    12121 acagaacaat gtggcggatg accttgccac gattcgtcgt ctgaacgaag acacggcggt
    12181 gaaaaataat caggcttcac agtcgaagga aagcgctgct gccagtgcga agactgcatc
    12241 agaaagcgcg aaggctgcga ccagcaaagc gacggagtct gcacaaaaag ccagtgaggc
    12301 tgcagaggcg gcgacccggg caggtgagtc tgaaaaggca gcggcagctg acgctaaaga
    12361 ggccagacga catgctgaaa ccgccagagt ggctcaggag gccgccggag acgttcttaa
    12421 acgtgctgag gctgctacag ttagtgctga agaagccaga cgtatggctg agaatgcacg
    12481 aggacctaaa ggcgatacag ggccgaaagg tgatgctggt cctcgtggtg aaacaggtcc
    12541 ggcgggtccg cgtggggaga aaggtgagca gggagagcgc ggacctcagg gcataccagg
    12601 ccagaaaggt gatacaggag aacggggacc tcagggagtg cagggtccag ctggcgcaac
    12661 agggccatct ggcccacgtg gtgcgaccgg gccacaggga gcaatggggc cgcgtggtga
    12721 aaccggggcc agaggcgaaa aaggagaccc gggggggcct cagggaccaa agggggatgc
    12781 tggtccgaga ggtgagccgg ggccgaaagg tgacaccggc ccacgtggag aggccggacc
    12841 acccggacca cagggtccgg cagggcaaac cggcccgaaa ggggataaag gtgaacccgg
    12901 cgcaacaggt ccggcaggtc ccgcaggccc tcggggagaa accggccccg caggtcctgc
    12961 gggtcccgca ggcagtgtcg ccagtgttcc ggatgccagc acatcacaga aaggtgttgt
    13021 acagctgagc agcgatacca acagcgatga tgaaacaaaa gcggcaaccc caaaggctgt
    13081 gaaagcggta atggcagaag tgcaggcggc aaaaacgaag gcagaagagg cagcaacccg
    13141 ggcagcagtc cccggaccga agggggacag gggggaacct ggcgcacctg gtgcagtggg
    13201 tcccgcaggc ccacggggac cggctggtgc agctggtccc aaaggtgatg caggccccgc
    13261 aggaaaggat ggtaccgccg gagcggaagg taaggcaggt ccggcagggc cacgaggtga
    13321 aaggggacca gcgggggcac agggtgttcc ggggccggtt ggtccggcag gtcctgcggg
    13381 taaaacgggg ccacagggcc ctgccggagg gcgaggaccg atgggactgc ccgggttgga
    13441 tggtaaaacc ggaccacagg gaccacaagg accacaggga ccacaagggc caaccggaag
    13501 gctggtaccc ggggaaattt acagcatcgg aacatacatt attgccgcac taggcccgta
    13561 tataaccgac atcggcagaa cctatcagcc aggagaaact gtatcgggtt cacgccttaa
    13621 gcgatgcgcc ctaatcaaag acgaaaacgg aaactatatg aaagctaaca ccgatggcat
    13681 tgacggcaac ctatcctcag ttccgggctc atggatggtg tgtaatgaaa ttacgtcgac
    13741 aaatgacaat gaaggcatcg ggctgttcca gcgcgcatac tgacagacag gagatgacat
    13801 gaatgtggaa aaaaataagc aatccgcaat gggcagataa agatcacact gctgttaatt
    13861 gcatggtgaa gtttgagcac attgagcagg cggttccttt cactgcaaca gccagcgata
    13921 ccgaagcata cgggcgggat atttatgccg cttgtctgag aggagaggcc ggtgaaattg
    13981 cagaatatgt acagccatcc atcagcccgg aaaaagccag agaactcaaa accgccggga
    14041 tcaacgcctg gcgtaatgcg atggaagcgg cgaactacac gtttgagcac aatgggcgta
    14101 aatgggacta cggcaaacag acacaggcgc gtcttgagcc gtcagtggct gccgcgaaag
    14161 caggaatact gccggagggc tttttctgga cggatgcgga gaataacgac gtcccgatga
    14221 cggcagaggc gctgatggca ctcagtgagg cggcggagaa ggcgatgttc accaaaggga
    14281 tggaaatcca cgtccggcag cgcaccatga agaaagagct ggaaaagctg accagtgcgg
    14341 atgagatact ggcatacagg gttggctggg cacaggaata acagataaaa atatggggga
    14401 aacatttctt tccctcatga actattcctc ctgtaaatat tttatgttaa ctgaatagtt
    14461 aattttttct gatgttgtaa ttttgttcac aaattacagt gtaagcagat gtgaaacagc
    14521 aagtctgact ggggtaagtt ttttctgata tctgttccgg cgg
//
LOCUS       DAZFRG010000113        14381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFRG010000113 DAZFRG010000000
VERSION     DAZFRG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.113"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..371)
                     /gene="yqfB"
                     /locus_tag="ACHKUC_002800"
     CDS             complement(60..371)
                     /gene="yqfB"
                     /locus_tag="ACHKUC_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO6619032.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAKQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            416..1849
                     /gene="bglA"
                     /locus_tag="ACHKUC_002801"
     CDS             416..1849
                     /gene="bglA"
                     /locus_tag="ACHKUC_002801"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO6619033.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            complement(2015..4888)
                     /gene="gcvP"
                     /locus_tag="ACHKUC_002802"
     CDS             complement(2015..4888)
                     /gene="gcvP"
                     /locus_tag="ACHKUC_002802"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO6619034.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVVDKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(5006..5395)
                     /gene="gcvH"
                     /locus_tag="ACHKUC_002803"
     CDS             complement(5006..5395)
                     /gene="gcvH"
                     /locus_tag="ACHKUC_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO6619035.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(5419..6513)
                     /gene="gcvT"
                     /locus_tag="ACHKUC_002804"
     CDS             complement(5419..6513)
                     /gene="gcvT"
                     /locus_tag="ACHKUC_002804"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO6619036.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            6804..6887
                     /locus_tag="ACHKUC_002805"
     CDS             6804..6887
                     /locus_tag="ACHKUC_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619037.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            complement(6961..8163)
                     /gene="ubiI"
                     /locus_tag="ACHKUC_002806"
                     /gene_synonym="visC"
     CDS             complement(6961..8163)
                     /gene="ubiI"
                     /locus_tag="ACHKUC_002806"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO6619038.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQWMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFSLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(8186..9364)
                     /gene="ubiH"
                     /locus_tag="ACHKUC_002807"
                     /gene_synonym="visB"
     CDS             complement(8186..9364)
                     /gene="ubiH"
                     /locus_tag="ACHKUC_002807"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO6619039.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQDPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(9361..10686)
                     /gene="pepP"
                     /locus_tag="ACHKUC_002808"
     CDS             complement(9361..10686)
                     /gene="pepP"
                     /locus_tag="ACHKUC_002808"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO6619040.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEHAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(10712..11290)
                     /gene="ygfB"
                     /locus_tag="ACHKUC_002809"
     CDS             complement(10712..11290)
                     /gene="ygfB"
                     /locus_tag="ACHKUC_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO6619041.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            11458..11787
                     /gene="zapA"
                     /locus_tag="ACHKUC_002810"
     CDS             11458..11787
                     /gene="zapA"
                     /locus_tag="ACHKUC_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6619042.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11829..12012
                     /gene="ssrS"
                     /locus_tag="ACHKUC_002811"
     ncRNA           11829..12012
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKUC_002811"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            12033..12635
                     /gene="fau"
                     /locus_tag="ACHKUC_002812"
     CDS             12033..12635
                     /gene="fau"
                     /locus_tag="ACHKUC_002812"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO6619043.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVVTPSKVWEW"
     gene            complement(12737..12796)
                     /gene="ibsC"
                     /locus_tag="ACHKUC_002813"
     CDS             complement(12737..12796)
                     /gene="ibsC"
                     /locus_tag="ACHKUC_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO6619044.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(13025..14257)
                     /gene="serA"
                     /locus_tag="ACHKUC_002814"
     CDS             complement(13025..14257)
                     /gene="serA"
                     /locus_tag="ACHKUC_002814"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6619045.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
BASE COUNT         3481 a         3880 c         3645 g         3375 t
ORIGIN      
        1 aatctccgaa aaatcatgaa attaaatgcg aaatttcaac taacaggcgt gagttcaatt
       61 taaaggcatt taaattcaat cacataaaac tgtgtctggt cgggatagat gtcggcaatg
      121 acctttttca gttcagtcag ggtcatattt tcctgctttg catgtttttc cgtcagcgta
      181 tccagcgtta cggttgaggt tgcggtgact tcaatcgtgc aaaaataacc gtcatcttca
      241 aaccgtccga cacgaagcac atcacccgtt ttgaagtgcg attcagactc gtcgcggatg
      301 gtgatggttt tacgcccagc cagaatgtca tcctggaaac gttgaaaaaa agtgatgtcg
      361 tttggctgca tggtaatatt tcctgtaaga attgactcat ctggagccta tgatagtgaa
      421 aaaactcacc ttaccgaaag atttcttatg gggcggcgca gttgccgctc atcaggtcga
      481 aggcggctgg aacaaaggcg gcaaagggcc gagcatttgt gacgttttga ccggtggcgc
      541 acacggcgtg ccgcgcgaaa tcaccaaaga agttgtgcct ggcaaatact atccaaacca
      601 tgaagccgtt gatttttatg gtcactacaa ggaggacatc aagctatttg ccgaaatggg
      661 cttcaaatgt tttcgtactt ccatcgcctg gacgcgcatt tttccaaaag gcgatgaagc
      721 tcagccaaac gaagaagggc tgaagttcta cgatgatatg atcgatgaac tgctgaaata
      781 caacatcgaa ccggtgatca ccctctccca ctttgaaatg ccgctgcatc tggtgcagca
      841 atatggtagc tggaccaacc gtaaagtggt tgatttcttt gtccgtttcg cggaagtggt
      901 atttgaacgc tataagcata aagtcaaata ctggatgacc ttcaacgaaa ttaacaacca
      961 gcgtaactgg cgtgcaccgc tgttcggtta ctgctgctcc ggcgtggtgt ataccgagca
     1021 tgaaaacccg gaagagacga tgtatcaggt gctgcatcac cagtttgtcg ccagcgccct
     1081 ggcggtgaaa gccgcgcatc gcatcaaccc ggagatgaaa gtcggttgta tgctggcgat
     1141 ggtgccgctc tatccttact cctgtaaccc ggacgatgtg atgtttgccc aggagtcgat
     1201 gcgcgaacgt tacgtcttta ccgatgtgca gttgcgtggc tattacccgt cctatgtgtt
     1261 gaacgagtgg gaacgccgcg gatttaacat caaaatggaa gacggcgatc tggatgtact
     1321 gcgcgaaggc acctgcgatt atctcggctt cagctattac atgaccaacg cggtgaaggc
     1381 cgaaggcggc accggcgatg cgatctctgg ttttgaaggc agcgtaccga acccgtatgt
     1441 taaagcgtct gactggggct ggcagattga tccggttggt ctgcgttacg cgctttgcga
     1501 gctgtatgag cgttaccaga agccgctgtt tattgtcgaa aacggttttg gcgcttacga
     1561 caaagtggaa gatgatggca gcatcaatga cgattaccgc attgactacc tgcgcgccca
     1621 tattgaagag atgaaaaaag cggtgactta cgatggcgtg gacctgatgg gctacacgcc
     1681 gtggggctgc atcgactgtg tgtcgttcac caccgggcag tacagcaaac gctacggctt
     1741 tatctatgtg aataaacatg acgacggtac tggcgatatg tcgcgttcac gtaagaagag
     1801 ctttgactgg tacaaagagg tgattgccag caacggcgag aatctgtaat tagccgatgt
     1861 gcctgatgtg ctccgctcat caggcctggc actcttttaa tatattgagc tcgcaatatt
     1921 ttatattacg cctcagccga aacacacaaa gcgcacttta ttaacaagcg aaaaaggcgc
     1981 cgaagcgcct ttataagata gtcgaatcag taaattactg gtattcgcta atcggtacgc
     2041 aggagcagaa caggttacgg tcgccgtaaa catcatccag acgtttcacg gtcggccagt
     2101 atttgtctgc cacacctgcc gggaatactg caacttcacg gctgtacgga tgcgcccact
     2161 cggcgaccag ttcgctctga atgtgcggcg cgttcaccag cgggttatct tccagcggcc
     2221 agacaccggc tttcacctga tcaatttctg cgcggatagc cagcatcgca tcgataaagc
     2281 gatccagttc cactttgctt tcagattcag tcggttcaac catcagcgta cccgccaccg
     2341 ggaacgacat ggtcggcgcg tggaaaccgt agtcgatcag gcgcttggca atatccagct
     2401 cgctgatgcc ggtttcttct ttcagcgggc gaatatcgag aatacattcg tgcgccacgc
     2461 gaccgtcgcg accggtatac agcaccggga aggcatcctg cagacggctg gcaatatagt
     2521 tggcgttaag aattgccacc tggcttgctt ttttcagccc ttctgcgccc atcatgcgga
     2581 tgtacatcca gctgattggc aggatagagg cactaccgaa cggtgccgca gaaaccgcgc
     2641 cctgacgggt taacatgcct tcgatttgca ccacgctatg acccggtaca aacggagcca
     2701 gatgcgcttt cacaccgatc ggtcccatac ccggaccacc accgccatgc ggaatgcaga
     2761 aagttttatg caggttaagg tgggaaacgt ccgcaccaat aaagcccggc gaggtgatgc
     2821 caacctgagc gttcatgttc gcgccatcaa ggtaaacctg accgccgaac tgatgcacga
     2881 cttcacacac ttcacggatc gtttcttcat acacgccgtg ggtagaagga taggtcacca
     2941 tgatacagga gaggttatcg cccgcctgtt ccgctttcgc gcgcagatca gtcagatcga
     3001 tgttgccgtt tttatcacac gccacaacca ccacctgcat tcctgccata tgcgcggaag
     3061 ccgggttagt gccgtgcgca gaagccggga tcaggcagat atcgcgatgc ccttcgttgc
     3121 ggctttcatg ataatgacga atcgccagca ggcccgcgta ttcgccctgt gcgccagagt
     3181 tcggctgcat acaaacggcg tcgtaaccgg tcagtttcac cagccagtca gccagctgcg
     3241 cgatcatctg ctgataacct tcagcctgtt ccggcgggca gaacgggtgc agttcggcaa
     3301 attccggcca ggtgatcggg atcatctcgg cggcggcgtt cagtttcatg gtgcaggaac
     3361 ccagcgggat catcgcctga ttcagcgcca gatctttacg ctccagcgag tgcatatagc
     3421 gcatcatttc ggtttcgctg tggtagcgat taaacaccgg atgggtgagg atttcgtcgt
     3481 cgcgcagcat cgcagcctga atagagcgac tgtcgtgagc cacgtctttg tccagtgtgt
     3541 cgatgtccag cccgtggtta tcgcccaaca gcacgctgaa aagctgcatc acgttttcgc
     3601 gcgtggttgt ttcatcaagg gtgatcccaa ccgcgttcag aatatcgcta cgcaggttga
     3661 tttcagctgc ttcggcacgc gccagcacgc ccgctttgtc gaccacttcc acacacaaag
     3721 tatcgaaata gtgcgcatgg cgcagcttca gacctttttg ttgcaggccc gccgccagga
     3781 tatcggtcag acggtggatg cggttagcga tacgtttcag gccaaccggg ccgtgataaa
     3841 cggcatacag gctggcgatg tttgccagca gtacctggga agtacaaatg ttggagttcg
     3901 ctttctcacg acgaatatgt tgctcgcgag tctgcatcgc catgcgcaga gcggtgttgc
     3961 cagctgcatc tttcgataca ccgataatac ggcccggcat tgagcgtttg tattcgtctt
     4021 tcgccgcaaa gaatgccgcg tgtgggccac cgtagcccat cggtacgcca aagcgttgcg
     4081 ccgaaccaaa aacaatatcc gcgccctgtt tacctggcgc agttaacagc accagcgcca
     4141 taatatcggc ggcaacgctg accacaattt tgcgtgattt cagttcgcta ataagcgcgg
     4201 tgtagtcgtg aatttcacca gtagtgccga cctgctgtaa cagcacgccg aaaacgtcct
     4261 gatggtcgag cactttttgc gcgtcatcga caatcacttc aaaaccaaag gtttcggcac
     4321 gagtacggac cacatccagc gtttgcggat gaacgtcaga agcaacaaag aagcggttgg
     4381 catttttcag tttgctgacg cgtttcgcca tcgccattgc ttcggcggca gcggtggctt
     4441 cgtcaagaag agaagcagag gccatatcca gcccggttaa atccagcgtt acctgctgga
     4501 agttgagcag tgcttcaagg cggccctggg agacttcagg ttgatacgga gtatacgcgg
     4561 tataccagcc cggattttcc agcatgttac gcaggataac cggcggtagc tgcacggcgg
     4621 tgtaacccat gccgatgtaa gacgtgaagc gtttattgcg actggcaata gccttgagtt
     4681 ctgccagtgc ggcgtattcg gtcgccggtg cgccaacctg cggcggagtc gcaagctgaa
     4741 tatctttcgg cacaatctgg ccggtcagcg cgtttaacga ttgtgcgcca acggcattca
     4801 gcatttcttg ctgttgcgcg gcgtccggtc cgatatggcg ttcaataaaa gcgccgctgt
     4861 tttcaagctg gcttaacgtc tgtgtcatga gcgatggttc ctgaaacgtg cagtgaattg
     4921 tgaacctctc tccttgcgaa gagagtgagg gtgaggcgta aatttcctca ccctgatcct
     4981 ctcccgcaga agaggaataa agccgttact cgtcttctaa caatgcttcg tatgcggttg
     5041 catccagcag tgattccagt tcgctttcat cactggcttt gattttgaaa atccagccgc
     5101 ctgcatacgg ttcgctgttc accagttccg gggaatcgct cagtgcatcg tttaccgcca
     5161 cgatttcacc gcttactggc gcataaatgt ctgacgccgc ttttaccgat tcggcaaccg
     5221 cgcagtcatc gcccgcgcta accgttgcgc ccacttccgg taggtcaaca aacaccatat
     5281 cgcctaacag ctcctgagcg tgttcggtga tgccaacggt gtaagtgccg tcggcttctt
     5341 tacgcaacca ttcgtgttct ttgctgtatt tcagttctgc tggtatgttg ctcatcaatc
     5401 aatctccaaa aaagtaaatc acgcgacggc tttgccgtta cgcacaaaaa caggtttcgt
     5461 cactttaacc ggcatttcac ggttgcgaat ttgcacaatc gccgtttcac caataccttc
     5521 cggcacgcgc gccagcgcaa tgctataacc cagcgttggg gagaaagtac cgctggtgat
     5581 aatgccttca tgctggttgc cctgcgcatc agtaaaacgt accggcagtt cattacgcag
     5641 cacgcctttt tcggtcatca ccagaccaac cagtttttct gtgccatgtt cacgctgcgc
     5701 ttccagagct tcacgaccga taaagtcacg atctgccggt tcccaggcga tagtccagcc
     5761 catattggcg gctaaaggag aaatagtttc gtccatctcc tggctataca aattcatgcc
     5821 cgcttccaga cgcagcgtgt cacgcgcgcc caggccacat ggcttaacgc cagcttccac
     5881 cagcgcgcgc cagaaatcgg ccgctttttc attgggcagc gcaatttcgt agcccgcttc
     5941 accggtgtaa ccggtggtgg caataaacag atcgcccgcc tgcacgccaa agaacggttt
     6001 catcccttcc accgcctgac gctgggcgtc attaaacagt gtggcagctt ttgcctgcgc
     6061 attcggccct tgtacggcga tcatggaaag gtcatcacga acagtaattt cgatgccgaa
     6121 aggttcagcg tgttgggtaa tccaggagag gtctttttcg cgggtagcgg agttaacaac
     6181 gaggcggaag aaatcttcag taaagtagta gacgatgaga tcatctatca caccgccaga
     6241 ggcattcaac atccccgagt aaagggcttt gccgcttttg gtgagcttcg ccacatcgtt
     6301 cgccagcaga taacgtagaa actcccgggt gcggctgccg cgaagatcga cgatggtcat
     6361 atgtgacaca tcaaacattc cggcatcggt acgtaccgca tgatgttcgt cgatttgcga
     6421 accgtaatgc agcggcatca tccagccgtg gaaatccacc atgcgagcgc cgcaaagcgt
     6481 gtgttgttcg tacaaaggag tctgttgtgc catcttgtcc tcattgaata agcggggctg
     6541 acaacttttc catggcgaaa ttatcaccac gaaacccagc atcggagcca ctcccggtcc
     6601 ccaacgcaat cgttctcttt tgcctgaact taccaccgaa acagactgtt aaccataagg
     6661 taaaattgat catcacatta gcttatggtt aaaaaatgca aaaatcgcaa cagaataaaa
     6721 aaccaaaaaa cacatcagtt tctatacaaa gatgatgtga tgagaaaatt aatttgaata
     6781 agacaatatt aagcgctaaa aaaatgtcaa aaaacactaa atcaaaaatc cataacatta
     6841 gaaaatataa tgcgaaaacg gaggttaaat tagtttattt caaatgagga aaatctcccg
     6901 gcgaaaaaac cgggagatga aagtgtgatg ggtatcaaat aaacagcaga agagaaatat
     6961 ttaacgcagc cattcaggca aatcgtttaa tcccattgcc tgacgaataa gttgtggctt
     7021 aacgccagga agcgtatcag ccagtttcag accaatatca cgcagcaatt ttttcgccgg
     7081 attggtaccg gaaaacagat cgcggaatcc ctgcatacca gccagcatca gcgccgcact
     7141 gtgcttacgg ctacgctcat agcgacgcag gtaaatgtac tgcccgatgt ctttaccctg
     7201 acgatgcaac cgtttcagtt cagcaatcag ctctgcagca tccataaagc cgagatttac
     7261 cccctgcccc gccagcgggt gaatagtatg cgcggcgtcg cccaccagcg ccagacggtg
     7321 tgcggcaaac tggcgcgcat aacgccccgt tagcgagaac accaggcgct cactttcgac
     7381 cttgcataag cccaggcgat tatcaaaagc gatatttaac gcgcgattaa attcgtcttc
     7441 gcctgcctgc tgcatccact gcgcttcctc tggcgacagt gaccagacaa tcgagcaaag
     7501 atgcggatcg ctaagcggta aaaaggccag aatgccttcg ccatggaaaa cctgccgcgc
     7561 caccgcatca tgcggttctt ccgtgcgaat ggtcgccacc agcgcgtgat gctgataatc
     7621 ccagaaagtc agcggaatat cggctttgtt gcgcaaccag gaattagcgc cgtctgcgcc
     7681 aatcaccaga cgcgccgtta acatgctgcc atctttcaac gtcaggaagg tttcattttc
     7741 tccccaggcg acctgctgta attctgcggg ggccaacaga gtgatatctg acgactgctg
     7801 cgctttgttc cacagcgcgt agtgaatcac tgagttttca acgatatgcc caagatggct
     7861 atagcccatg ctttgatcgt caaacgagat gtgaccaaaa ctgtctttat cccacacttc
     7921 cataccgtga tagcaacagg ccctacgaga gagaatgtcc tgccagacgc caagacgggt
     7981 gagtaatttt tcgctggcgg cattgatagc cgaaacgcgc agttgtggtg gtgcatccgc
     8041 cgccagaggt tccggtacgc gctgctccag tacggcaacg cgtaagccgc taccctgtaa
     8101 tccgcaggca accgccagcc cgaccatgcc gccgccaaca atggctacat caacactttg
     8161 cattgtttat tccttaaaac cgccttcaac gcgccaccca accaagagtg cgctgcgcca
     8221 gcacatcgcg tgctggggta aataattcca tcgtcatcag cccgatgttg cgcccgacaa
     8281 ccagcggcgt ccaacggttg gcaaaaagat gtacaaggct gtccgtgacg ccgatggttg
     8341 cttcgcgatc gctctgtcga cgctgctgat aacggcacaa tacgccgtaa tcacccatgt
     8401 cttctccgcg ctcctgcgcc tgagtcaggg tttctgcaag gctcatgaca tcacgcatac
     8461 cgaggttaaa tccttgcccg gcgatggggt gcagagtttg cgccgcattc cccaccagca
     8521 cggtacgatg ggtaataggt ttggcggcgc gggttaacgc cagcggataa gcactgcgtt
     8581 taccggcgtg agtaattttc ccaagtcgcc agccaaaggc cgactggagt tcacggcaaa
     8641 acttctcgtc gctccacgac agcacctctt cgcgccgctc cagtggatga caccagacca
     8701 gcgaacagcg tccgtcagac atcggcaaca tcgccagcgg gccatgttgc gtaaagcgtt
     8761 caaaagcgcg cccttcatgc gcaacggaag tcgcaacgtt cgcaatcaca gccagttgtt
     8821 cgtaaggatc ctgctgccag tcaacgccgc acacggtggc taacgctgaa tgggtgccat
     8881 cagcggctac cagcacgcgg cccgtcagcg tctcaccact ctccagcgtc acttcaacgt
     8941 ggctctgagt acgggcaacg ttagccacgc gatcagggca atgcagcgtt acgccaggtg
     9001 ctttgcgcag caatgcaaac agccgttgcc cgacattgtg caattcgaca acctgcccca
     9061 gcgccgccag ttggtaatct tctgcggcga gggtgacaaa tccagcgtga ccacgatcgc
     9121 tgacatgcac ggtggtgatg gcagttgcgc aatcagccag agattgccag acgccgatgc
     9181 gcgccagttg ctgacaggta cccgccgcca gcgcaatagc tcgtccatca aagcccggat
     9241 gagcatgtga ctctggcgcg gtcgcttcaa tcaaatgtac tggcagcgcc ccgtgactta
     9301 accgggaaat agccagcgcc agcgtcgcgc ccgccatgcc gccaccgacg atgattacgc
     9361 tcattgcttt ctcgcagctg ccatcaacgc ttcgatttct tccggctttt tcaccacgct
     9421 ggcggtgagg ttttcattac cggtttcggt aatcacaata tcgtcttcaa tacgaatgcc
     9481 gataccgcga tactgttctg gcacttctgc atccggcgca atatacagcc ctggttctac
     9541 ggtcagtacc atacccggtt ccagaatacg cgagcgatcc tgaccataaa caccaacgtc
     9601 atgaacatcc agtcctaacc agtggctaag gccatgcata aagaaaggac gatgggcgtt
     9661 ctgagcgatc agttcatcaa catcaccttt caggatgccg agttttacca gaccgctaac
     9721 catgatgcgc accacttcac cagtgacttc ctgaatggaa gttcccggac gatacaggcg
     9781 caggctggtt tcaagagact ccagcacaat gtcatagatt tcacgctgag cctgagtgaa
     9841 tttgccgttg accgggaagg tgcgggtaat gtcgccagcg taacctttgt attcacaacc
     9901 cgcgtcaatc aacaccaggt caccgtcacg cagttcacac tcgttttcgg tgtagtgcaa
     9961 aatgcagccg ttttcaccgc tgccaacaat ggtgttatag gaagggtaac gcgcaccgtg
    10021 gcggttaaat tcgtggtgaa tttcgccttc cagatggtac tcgaacatcc ccggacggca
    10081 tttttccatc gcccgtgtat gcgccagtgc ggtgatttca cccgcgcggc gaagtacggc
    10141 aatctcttct ggtgatttga acaggcgcat ttcatgaaca acaggacgcc agtcgatcat
    10201 cgttgccggt gcggtgagat tttgccgcga acctttacgc agtttctcaa gcgcgctgtt
    10261 cacgatttca tcagcatatg catgttcgcc ctgagcgtgg taaaccacat ccagaccatt
    10321 aagtagttga taaagttgct gattgatttc gctgaatgcc agtgcgcggt caacgcccag
    10381 tttctctggc gcggcatcct ggcctaagcg acgaccaaac cagatctctg ccgtcaggtc
    10441 gcgaacgcgg ttaaacaaaa cgctgtggtt atgagtgtca tcgcttttaa tcagcaccag
    10501 caccgcttcc ggttcgttaa agccggtgaa gtaccagaag tcactgttct gacgataggg
    10561 gtattcgctg tcggcgctac gtgttacttc tggtgcagca aaaatcagcg cggcgctgcc
    10621 gggttgcatt tgctctacca gggcctgacg gcgacgctga aactcttgcc gggatatctc
    10681 actcataaca ctctccttac gttttttgtt tttagtgtag agtcggtttt tgtacttctg
    10741 gcgcggtcgg ttgcggatga gtaaaggtgt cgtggcataa cagcgcggca acacgaacgt
    10801 attcgatgat ctcttcaagc gacatttcaa gctcttcctg atcttcgtct tcgtcgtaac
    10861 ccaactgcgc gatgttacgc agatcgtcga tggcttcacc ggtttctccg gtcactttgt
    10921 ccagcttcgg ttgcgtaacg ccaagaccaa gcaggaagtg attgacccaa cctgccagcg
    10981 catcagcccg atcgaaaacg ctgacatcat cgccatcagg cagataaagc tgaaaaagga
    11041 agccgtcatc ctgcagggca tcgctggtgg cagagtgcat tttacgcagt gcctgtgcca
    11101 gctcatgacc gaaagccatg ccttcgttcg tcaggtcgtg aagtagcggc agccatgagc
    11161 tatcatcgtt accgccacat atcatcccac tgattaaacc atgcatctca gcaggggtca
    11221 gacccgtccc ttgttggttc agatactggt tcatttcgtt gtaaccaggc atttcgttct
    11281 gtatagacat aagcattcgt cgtcaaaggg aggaatattc atgatatgct accactttgg
    11341 gccctggtgg accagaaaag ggcttgtctc ttctcatcag ggtagctata gtgtcgcccc
    11401 ttcgcagacc atgggtctaa agacgaaggc agcgcagtca atcagcagga aggtggcatg
    11461 tctgcacaac ccgtcgatat ccaaattttt ggccgttcac tgcgtgtgaa ctgcccgcct
    11521 gaccaaaggg atgcgttgaa tcaggcagcg gacgatctga accaacggtt gcaagatctg
    11581 aaagaacgca ctagagtcac aaatactgaa cagttggtct tcattgccgc attgaatatc
    11641 agctatgagt tagcgcaaga aaaagcgaag actcgtgact acgcggcaag tatggaacag
    11701 cgtattcgga tgctgcagca gaccatagaa caagcgttac ttgaacaagg tcgcatcacc
    11761 gaaaaaacta accaaaactt tgaatgacac ttttcggttt actgtggtag agtaaccgtg
    11821 aagacaaaat ttctctgaga tgttcgcaag cgggccagtc ccctgagccg atatttcata
    11881 ccacaagaat gtggcgctcc gcggttggtg agcatgctcg gtccgtccga gaagccttaa
    11941 aactgcgacg acacattcac cttgaaccaa gggttcaagg gttacagcct gcggcggcat
    12001 ctcggagatt cccttcttac ctggcaccag ccatgacgca actaccagaa ctcccactga
    12061 cattatcccg acaagaaatc cgcaaaatga ttcggcaacg tcgtcgtacg ttaacgccgg
    12121 aacaacagca ggaaatgggt caacaagccg ctacccggat gatgacgtat cccccagtgg
    12181 tgatggcgca tacggtcgct gtattcctct cttttgatgg cgaactcgac acccagccac
    12241 tcatagaaca actctggcgc gccggtaagc gcgtatatct tccagttttg catcccttta
    12301 gtgccggtaa cttgctgttc ctgaattacc atccgcaaag cgaactggtg atgaacaggt
    12361 tgaagatcca cgagccaaaa ctggacgtgc gcgatgtgct tcccctttcc cggttagatg
    12421 tattgatcac gccgctggtg gcttttgatg aatacggtca acgcttggga atgggcggtg
    12481 gtttttatga tcggacctta caaaactggc agcactataa aatgcaaccg gtgggttatg
    12541 cgcatgattg ccagttagta gaaaaactcc cagtggagaa atgggatatt ccactgcctg
    12601 cggtggttac accgtcgaaa gtctgggaat ggtaagggcg atacatcccg catcgccctg
    12661 attgacatcg ttgattcttt gacctaattt agtgagtaag ggtaagggag gattgttcct
    12721 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    12781 tgacaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    12841 tacagcccca tgctgccctg ccatcgtaaa tccccattaa ataaacacaa cgcattgatc
    12901 tgactttgat ttattttctg gagcagactc gcaaagtaga atgcgcaatg cggcaacggt
    12961 gtggagaagg gataaaaaaa cgggcaagtc agtgacctgc ccgttaattt tcagagaagg
    13021 ggaattagta cagcagacgg gcgcgaatgg tacccggaat agctttcatt gcctgcagcg
    13081 ctttttcggc aacgtcttcg tcggcttcaa tatcaataac tacataaccc atctgggcgg
    13141 aagtttgtag atattgcgcg gcgatgttga cgccctgctc ggcaaaaatt ttgttgagcg
    13201 cagttagcac gcccggacgg ttttcgtgga tgtgcatcag acgacgccca ccgtgcagtg
    13261 gcagcgagac ttccgggaag ttcaccgcag agagcgttga gccattgtca gaatacttga
    13321 tcaatttacc cgcaacttcc aagccgatat tctcctgcgc ttcctgagtc gaaccgccaa
    13381 tgtgtggcgt cagaaggacg ttgtcaaatt cacacagcgg agaggtaaat ggatcgctat
    13441 tggtcgccgg ttcagtcggg aatacgtcga ttgccgcccc cgccagatgt ttgctcgcca
    13501 gcgcgtcaca cagcgctgga atatccacca cagtaccgcg cgaagcatta atcagcagcg
    13561 agccgggttt cattagcgaa atctctttcg cgcccatcat atttttggtg gacggattct
    13621 ctggtacatg cagactcacc acatcgctca tattcagcag gtcagaaaga tgctgtacct
    13681 gagtggcgtt gcccagcggc agtttgtttt caatatcata aaagtaaaca tacattccca
    13741 gcgattcagc cagaatgccc aattgcgtac caatatgacc gtagccgatg atacccagct
    13801 ttttgccgcg cgcttcaaaa gaacccgccg ccagtttgtt ccacacgcca cgatgcgctt
    13861 tagcattggc ttccggcacg ccgcgcaata gcagcagcag ttcgccaatc accagctccg
    13921 caacagagcg cgtatttgag aacggtgcgt taaataccgg gatcccgcgc tttgccgccg
    13981 catccagatc aacctggttt gttccgatac agaaacagcc aatagcgacc agtttttctg
    14041 cggcgttgat cacgtcttca gtcagatggg tacgggatcg caggccgatg aagtgggcat
    14101 cgcggatgga ttcttttaat tgttcatcat ccagcgcgcc tttgtgaaat tcgatgttgg
    14161 tgtaaccagc tgcacgaagg ctttccagcg ccttttggtg cacgccttct accagcagaa
    14221 acttaatctt gtctttctcc agcgatacct ttgccattta cccaatcctg tcttttgaaa
    14281 tgttgtgtgc ggatttgcat ccgcctttca acatatcaaa aaataatatt gcggcaatat
    14341 gaacgtttgc gtcgcgatgt tgaggaaata tcacgcaaaa g
//
LOCUS       DAZFRG010000114        14368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFRG010000114 DAZFRG010000000
VERSION     DAZFRG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.114"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..833
                     /locus_tag="ACHKUC_002815"
     CDS             6..833
                     /locus_tag="ACHKUC_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6619046.1"
                     /translation="MLLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYP
                     EYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNL
                     AFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNY
                     GAANENEWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIA
                     SSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1051..3483)
                     /gene="metL"
                     /locus_tag="ACHKUC_002816"
     CDS             complement(1051..3483)
                     /gene="metL"
                     /locus_tag="ACHKUC_002816"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO6619047.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3486..4646)
                     /gene="metB"
                     /locus_tag="ACHKUC_002817"
     CDS             complement(3486..4646)
                     /gene="metB"
                     /locus_tag="ACHKUC_002817"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO6619048.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEHTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4923..5240
                     /gene="metJ"
                     /locus_tag="ACHKUC_002818"
     CDS             4923..5240
                     /gene="metJ"
                     /locus_tag="ACHKUC_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO6619049.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5300..5908
                     /gene="yiiX"
                     /locus_tag="ACHKUC_002819"
     CDS             5300..5908
                     /gene="yiiX"
                     /locus_tag="ACHKUC_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO6619050.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAAGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5969..6181)
                     /gene="rpmE"
                     /locus_tag="ACHKUC_002820"
     CDS             complement(5969..6181)
                     /gene="rpmE"
                     /locus_tag="ACHKUC_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6619051.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6384..8582
                     /gene="priA"
                     /locus_tag="ACHKUC_002821"
     CDS             6384..8582
                     /gene="priA"
                     /locus_tag="ACHKUC_002821"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO6619052.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSIWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTNHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8738..9763
                     /gene="cytR"
                     /locus_tag="ACHKUC_002822"
     CDS             8738..9763
                     /gene="cytR"
                     /locus_tag="ACHKUC_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO6619053.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9855..10814
                     /gene="ftsN"
                     /locus_tag="ACHKUC_002823"
     CDS             9855..10814
                     /gene="ftsN"
                     /locus_tag="ACHKUC_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO6619054.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLVQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPAYTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10907..11437
                     /gene="hslV"
                     /locus_tag="ACHKUC_002824"
     CDS             10907..11437
                     /gene="hslV"
                     /locus_tag="ACHKUC_002824"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO6619055.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11447..12778
                     /gene="hslU"
                     /locus_tag="ACHKUC_002825"
     CDS             11447..12778
                     /gene="hslU"
                     /locus_tag="ACHKUC_002825"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO6619056.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12845..13771
                     /gene="menA"
                     /locus_tag="ACHKUC_002826"
     CDS             12845..13771
                     /gene="menA"
                     /locus_tag="ACHKUC_002826"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO6619057.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13864..14349
                     /gene="rraA"
                     /locus_tag="ACHKUC_002827"
     CDS             13864..14349
                     /gene="rraA"
                     /locus_tag="ACHKUC_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO6619058.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         3498 a         3813 c         3819 g         3238 t
ORIGIN      
        1 tatccattct attaatacct tgcattggaa acgctcagga aattaaaatt gatgaaagct
       61 ggttacatca aagcttgaat gtcattggtc gcacagactc tcgctttggc ccaagactga
      121 ctaacgacct ctaccctgaa tatactgtag caggaagaaa agactggttt gatttttatg
      181 gttatgttga tctaccgaaa ttctttggcg tcggcagtca ctatgatgtt gggatctggg
      241 atgagggctc accactattt acggaaatag aacctcggtt ttccattgac aaattgaccg
      301 gattaaatct tgcgttcggc ccatttaaag aatggttcat tgcaaacaac tatgtctatg
      361 atatgggtga caaccagtca tcccggcaaa gtacatggta tatggggctt ggtacagata
      421 tcgacacggg tctaccaatt aagctttctg ccaatatata cgccaagtat cagtggcaaa
      481 actatggtgc cgctaatgaa aatgaatggg acggatatcg attcaaaata aaatatagca
      541 tccctcttac aaatttattc ggagggcgat tggtatacaa tagttttact aactttgatt
      601 ttggctccga tcttgcggac aagtcacaca ataataaacg aaccagtaat gctattgctt
      661 caagccatat cctttccctt ctatatgaac actggaaatt tgcatttaca ctacgttatt
      721 ttcacaacgg tggacaatgg aatgcgggag agaaggttaa cttcggagat ggtccatttg
      781 aattaaaaaa tacaggatgg ggaacctata ctactattgg ttatcaattt taaattaggt
      841 gacattttag atattgttac ttaatgtcat tttgtaattg tcattaataa cttcaccatt
      901 cactctgtta cttcatcgct caacacaaaa gcccccttat taccgggggc actgaccaaa
      961 ggcaaaatga caatcaatca ccacttcccc aggaagaggt ttaggggtga caacaaaagc
     1021 tggcaactgc cagcttttgt tcaataatca ctacaacaac tgcgccagtc ggttaatatc
     1081 cgactgaatc gccccggcgg tgacgtcgcg ccctgcacct ggcccgcgga tcaccagcgg
     1141 gttgtcgcga taccagcggc tttcgatggc aaagacgtta tcgcatggca gtagtgatgc
     1201 cagcggatgg tcttcacgca ccgcttccac gcccacacgc gctttgccgt tggcatcgaa
     1261 acgcgccacg tagcgcagca ccagccccat ttcgcgggcc gcttccagtc gttgcaccat
     1321 ctgctcgtta agttcatcgc cattttcaaa gaaatggtcg atgctgccgc cttcgcaatg
     1381 ggcaggcacc agtgattcaa cacgtacctg gtccggttca atgttgtaac ccgcttcacg
     1441 cgccagaatc accagcttgc gcatcacgtc tttgccggaa aggtcatcac gcgggtcagg
     1501 ttcggttaag ccctgctgcc acgcctgatc caccagttcg gtaaacggca cgctaccgtc
     1561 gaattgcagg aacagccagg agagcgtgcc ggagaagatc ccactgatcg acaaaatggt
     1621 atcgccgctg tcgatcagat cgcgcacggt atggttgatc ggcaagcctg cgccgacggt
     1681 agcgttgtac agccagtgac gcccggtttt ttcgaaggcg tcgtggatct ggcgatattt
     1741 attgctgtcg ctcgctcctg ccagtttgtt ggcactgatg acgtggaaac cgtggctggc
     1801 gaaatcaaga tactggtcag cgagctgctg gctggcggta acgtccagca ccactaaatc
     1861 atcatacgga tgggcgcgca tccacaggaa cagcgactct tcatcctgct cgaccgcttc
     1921 atcgttgaag aaggctaacg cgcggctggc gtccagaccg tcatagctca acaggctacg
     1981 gcggctgtcc accacgcctg ccagcacaaa ttcaaagccg gtacgtgccg aaagcgtact
     2041 ctgctcacgg gcgaacagtt ccagccagcg ggaaccgata ttgcccttgc cgaacaacac
     2101 caggccgatg cgtttttccg cgcggaagac ggactgatgc agcccctgaa tcaggctttc
     2161 ggtcgggccg gtgcgcagta ctgccaccag gctgatgcca tcatcggact gccaggtaaa
     2221 ctcgaccggc tggcctttca gttgctgcca gaagcggtgg caatgcagcg ggttacgggt
     2281 gacgcccgcc ccaaccatcg ccaccagcgc cagcccctga cgcaggcgca gttcgccagg
     2341 taatcccgct tcgtcgagga ttttcagcgc actgtcggcc acttctgagg tgtagcaaaa
     2401 ttgcagcaac tggcgatcgt tatgcacgcc aaccgccagt gggcgcacct gagcacgttt
     2461 caggatctgg tcgatctctt tatgcgccag tttgaaatcc tggctggcgg gcacctgaaa
     2521 ctcaatcaag cagacatcat catggctggt gacaatacgt gcaccagtac cggaggccag
     2581 cacgcgttca atgcgcgtgg aaccttgatc cggcgtgtag ctacagcgca gttgcaagtc
     2641 gatttcgctg ccagaaacgg gctgtaaagt acgggcatga agaacgggag ccgccagacg
     2701 cgccagttcg ctggcttcat ccagacgcag caacggcagc aggcaggcat ctttcacttt
     2761 acgcgggtcg gcgctatata ccccggcgac gtcgctccag atggttacgc gagaaacacc
     2821 cgccagcgca ccgatttgtg tcgcggaata gtcggaaccg ttacgcccca gcagcaccgt
     2881 ttcaccggcg ttgttacggc tgataaaccc ggtcaccacc agacgtttgc ccggatgttg
     2941 taccagtagc tgttgcagca acgggtaaga aagtccttca tcaacctgcg gttgcgcagc
     3001 gcgttcagcg cgtaaaaact cgcgggcatc aagccaggcc gctggcagcc cttgttgatt
     3061 aagcaccgca gacatcaggc gcgccgacca cacttcaccg tggcccacca cttccgcata
     3121 caccgcatcg ttaataccgc tgtcgagcaa cgccgccagg cgctcaagat cgctgacaaa
     3181 agcgctaatg aggttatcgg cttcttcggc gggtagcagg ccgctaatca gatcgcactg
     3241 ataacgacgt aatgtttgtt gaacctgatg cgcagagaga cgatcggtct ggcttagttt
     3301 caaccagtta atcaactggt tagtggtgct accggcggcg gaaaccacca tcatatcgtc
     3361 aggctgagag tactccgcca taatgcccgc gacacgcaaa taacacttca catcagccag
     3421 actactgcca ccaaatttat gcagttgacg acctttcgcc cctgcctgcg caatcacact
     3481 catttttacc ccttgtttgc agcccggaag ccattttcca ggtcggcaat taaatcttcg
     3541 ccatcttcaa taccggtgga gatacgcagc agcgtctcgg agatcccggc ggcagcacgc
     3601 gcttctggcg ccatgcctgc atgtgtcatg gttgcggcgt gagagattaa actttccact
     3661 ccccctaatg attccgccag cgtaaacaac gacagcccgc ccaggaaacg acgcagcgtg
     3721 tgctcatcgc catccagttc aaaactcaac attgcgccaa agcctttttg ctggcgcgcg
     3781 gcaatttcat gcccctgatt ttccggcagc gacgggtgat acagtttttt caccaacggc
     3841 tgggtttgca gatatttcac aatcgcctgc gcgttgcgct gggccagctc cattcgcggc
     3901 accagcgttc gcaacccgcg cagcagcaga tagctgtcaa acgcgccgcc cgtcacgcca
     3961 atattgtttg cccaccaggc aagttcagtg acaacgtccg ggtctttagc aatcactacg
     4021 cctgccacca cgtcagagtg accgttcaga tatttcgtgc atgaatgcaa caccagatcg
     4081 gcacctaatg ccagtggatt ttgtaatgcc gggcttaaga aggtgttatc caccacgctc
     4141 accgccccga cttcccttgc cagatggcag attttcgcaa tatccacgac gcgtaacaat
     4201 ggattacttg ggctttctac cagtaccagt ttggggtttt ctgccagcgc tgcccgtaat
     4261 gcctgttcat cgctttgatc aacaaacaac acgcgatagc aaccgcgttt cgccaggctg
     4321 tcgaacaggc gatagctacc gccgtagcag tcgtgcggcg caaccagcag atcgccaggt
     4381 ttcaaaaaga cggtcgttac caggtgaatc gcggacatgc cggtattagt aagtactgca
     4441 ccagcaccgc cttccagttc tgccagcgct cgctgaacca catcgcgcgt tgggttgccg
     4501 cgccgcgagt aatcatgcgc gcgcggttca ttaaatccgg taaagttata ggtactggaa
     4561 agatggatgg gagggacaac gcaaccatac tgttcgtcgt catttaaccc gctacgcact
     4621 gcgatggtgg cctgtttacg cgtcatgtga tgaagttccc tgggcttggt cggtgaaatg
     4681 tcaggcacca gagtaaacat tgtgttaatg gacgtcaata catctggaca tctaaacttc
     4741 tttgcgtata gattgagcaa atctcaaata gccgttaaaa ttatatgcat tatcacgccg
     4801 acaggtgcat tacacgatgt cacggtaacg cctgtacggt aaactatgcg ggtttacggt
     4861 cagtacccat attaattgtg tggtctggcc tcaatttatt gacgaagagg attaagtatc
     4921 tcatggctga atggagcggc gaatatatca gcccatacgc tgagcacggc aagaagagtg
     4981 aacaagtcaa aaaaattacg gtttccattc ctcttaaggt gttaaaaatc ctcaccgatg
     5041 aacgcacgcg tcgtcaggtg aacaacctgc gtcacgcgac caacagcgag ctgctgtgcg
     5101 aagcgtttct gcatgccttt accgggcaac ctttgccgga tgatgccgac ctgcgtaaag
     5161 agcgcagcga cgaaattccg gaggcggcaa aagagatcat gcgtgagatg gggattaacc
     5221 cggagacgtg ggaatactaa agcggaaaag agcggcgcgg agttaaccag ccgctttttt
     5281 catacatgga cgtttaacga tgaaaatcag gctactgatt ctcagcctgc tggtttctgt
     5341 gcctgcattt gcctggcaac cacaaaccgg cgacatcatc tttcagatct ctcgctcatc
     5401 gcaaagtaaa gcgatccaac tggcgaccca ttccgattac agccacaccg gtatgctggt
     5461 gatacgcaac aaaaagccct acgtttttga agcggccggc ccggtaaaat acaccccact
     5521 caagcagtgg atcgcccatg gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg
     5581 actgagtgtt gaacaacagc aaaaactggc gcaaacggca aaacgttatc tcggtaaacc
     5641 gtatgactcc agcttctcgt ggagcgacga tcgccagtac tgttcggaag tggtgtggaa
     5701 ggtctaccag aatgcgctgg gaatgcgtgt gggcgagcag cagaagctga aagagttcga
     5761 tcttagtaac ccgctagttc aggcaaagct caaagaacgc tacggcaaaa atattccgct
     5821 tgaggaaacg gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggtcgc
     5881 caaagaatgg ccgctgtttt cctggtaata cgaccaaaat tcaggcacaa aaaaagcgcc
     5941 gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt
     6001 tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt
     6061 ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg attttcatta
     6121 cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca
     6181 tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg
     6241 atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa
     6301 ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc
     6361 gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc
     6421 ctcgtacctt tgactatctg ctgccagaag gcatgacggt taaagccggg agtcgcgtgc
     6481 gcgtgccgtt tggcaaacaa caggagcgca tcggggttgt ggtgtcagtg agcgatgtca
     6541 gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt gagccggtat
     6601 ttactcactc catctggcga ttactgctct gggcggcaga ttactatcat catccgattg
     6661 gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct gcggcgaacg
     6721 cgccgatgtg gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga
     6781 aacgttcgcc aaagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg
     6841 accaggttgc cacgctcgaa tttaatgatg ccgcgttgca ggcactgcgc aaaaaaggtc
     6901 tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt
     6961 ctggtgagcg gttgcggttg aataccgaac aggccaccgc cgttggcgca attcatagcg
     7021 cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg
     7081 tttacctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc
     7141 cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacgtttt aatgccccgg
     7201 tggaagttct gcattccggc ctgaacgaca gcgagcgtct ttcggcgtgg ttgaaagcga
     7261 aaaatggcga ggcggcgatt gtcatcggca cccgctccgc gctgtttacg ccgtttaaaa
     7321 atctcggcgt gattgttatt gatgaagagc acgacagctc ctacaagcag caggaaggct
     7381 ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccaatta
     7441 ttcttggctc tgcaacgccc gcactggaaa cgttatgcaa cgtgcagcag aaaaaatacc
     7501 gcctgctgcg cctgacccgt cgggcgggca atgcgcgccc ggcaattcaa catgtgctgg
     7561 atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc
     7621 agcatttaca ggccgacaac caggttatcc tctttcttaa ccgccggggt tttgcgcctg
     7681 cgctactgtg ccacgactgt ggttggatag ccgaatgccc acgttgcgat cactactaca
     7741 cgctgcatca ggcgcaacag catttgcgct gccaccattg cgacagtcag cgtccggtgc
     7801 cgcgccagtg cccttcttgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac
     7861 agcttgaaca gacgctcgcg ccgttgttcc ctggtgtccc catttctcgt atcgaccgcg
     7921 ataccaccag ccgcaaaggc gcgctggaac aacaactggc ggaagtacat cgcggtggcg
     7981 cgcggatttt gattggcacg caaatgctgg cgaaaggtca ccactttccg gatgtgacgc
     8041 tggtggcgtt actggatgtg gacggcgcgc tgttttctgc ggattttcgc tcggcagagc
     8101 gttttgccca gctttatacc caggtggcag gccgtgccgg gcgtgcgggt aaacagggtg
     8161 aagtagttct gcaaacgcac catcctgaac atccgctgtt gcaaacgttg ctctataaag
     8221 gctacgacgc ctttgccgaa caggcactgg ctgaacgacg catgatgcaa ctgccgccgt
     8281 ggaccaacca tgtgattgtg cgtgcggaag atcataacaa tcagcacgcg ccgctgttcc
     8341 tgcaacagtt gcgtaatctg atcctcgcca gcccgcttgc agacgacaaa ctgtgggttc
     8401 tcggtccggt tccggcactg gcccctaagc gtggtggccg ctggcgctgg caaattttgt
     8461 tgcagcaccc ttcccgcgtg cgattacagc acatcatcaa tggtacgctg gcgcttatta
     8521 atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgtcgatccc atcgagggct
     8581 aatgcgcaaa cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa
     8641 attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg
     8701 gaagtagtac gcctgccgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac
     8761 tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ccgtctcccg
     8821 agcattaatg aatcccgata aagtctccca ggccactcgt aatcgggttg aaaaagcggc
     8881 ccgggaagtg ggttatttac cacagcctat ggggcgcaac gtcaagcgta atgaatcccg
     8941 taccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg
     9001 tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact gtgcgcatca
     9061 aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt
     9121 gttgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc
     9181 gatggtgatg gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa
     9241 tctgaccgcc gcatttgatg cagtaaatta tttatatgag caagggcata atcggattgg
     9301 ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag gctacgtcca
     9361 ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg gcgacttcac
     9421 cttcgaagct ggaagcaaag caatgcagca gctgcttgat cttccacaac cgcctactgc
     9481 tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg
     9541 gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt
     9601 ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat
     9661 gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga
     9721 ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact
     9781 gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat
     9841 acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc
     9901 ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta
     9961 tggtcgctat tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc
    10021 atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac
    10081 caccaaaacc agaagaacgc tggcgctaca tcaaagagct ggaaagtcgc cagccgggtg
    10141 tgcgtgcgcc tacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac
    10201 cagaacaacg ccagcttctt gagcaaatgc aggctgatat gcgccagcag ccaacgcagc
    10261 tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacagcgcc
    10321 agcgtcaggc gcagcaatta gcggagcagc aacgcctggt tcagcagtcg cgtacgactg
    10381 aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc
    10441 gccagtctaa accggcttat actcagcagc cgtaccagga tctgctgcaa acccctgcgc
    10501 acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc
    10561 caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca
    10621 gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaaggc tttgactcaa
    10681 aaattaccac caacaatggc tggaatcgtg tggtgattgg cccggtgaaa ggcaaagaga
    10741 acgcagacag caccctcaat cgcttgaaga tggcgggtca tacaaactgc attcggctcg
    10801 ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact
    10861 tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt
    10921 aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa
    10981 taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc
    11041 gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact
    11101 ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcgaaag actggcgtac
    11161 cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct
    11221 tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc
    11281 cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc
    11341 ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca
    11401 tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac
    11461 cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg
    11521 ttccgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg
    11581 ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga
    11641 aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa
    11701 attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg atctgaccga
    11761 tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga
    11821 actggcagaa gaacgtattc tcgacgtact gatcccaccg gctaaaaaca actggggaca
    11881 gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa aactgcgtga
    11941 aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga
    12001 aattatggct cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa
    12061 cctgggcggt cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct
    12121 gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag atgctatcga
    12181 cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg
    12241 cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt
    12301 agaaggttgc actgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat
    12361 cgcttctggc gcgttccaga ttgcgaaacc atctgacctg atcccggaac tgcaaggtcg
    12421 tctgccaatc cgcgttgagc tgcaggcact gaccaccagc gacttcgagc gtattctgac
    12481 cgagccgaat gcctctatca ctgtgcagta caaagcactg atggcaactg aaggcgtaaa
    12541 tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc aggtgaacga
    12601 atctaccgaa aacatcggtg ctcgtcgttt acataccgtt ctggagcgtt taatggaaga
    12661 gatttcctac gacgccagcg atttaagcgg tcaaactatc actattgacg cagattatgt
    12721 gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc
    12781 gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg
    12841 tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa
    12901 aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg
    12961 tcacttcgat ccactggtcg ccctgctggc attgattacc gccgggctat tacagatcct
    13021 ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat
    13081 tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga aacgggcgct
    13141 cattattacc gttgtgctca tctgtctttc cgggctggca ctggttgcag tggcgtgcca
    13201 tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat
    13261 cacctacacc gtgggcaatc gtccttatgg ttatatcggt ttgggtgata tttccgtact
    13321 ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc atacattgat
    13381 tccggcactg atccttccgg caaccgcatg cggcctgctg gcaacggcag tactgaacat
    13441 taataacctg cgtgatatca atagcgaccg cgaaaacggc aaaaacacgc tggtggtgcg
    13501 cttaggcgca gtaaacgcgc gtcgttatca tgcctgcctg ctgatgggct cactggtgtg
    13561 tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc
    13621 ggcaccgttg ctggtgaagc aagctcgtta tgtgatgcgc gaaatggacc cggtggcgat
    13681 gcgaccaatg ctggaacgca ctgtcaaagg agcgttactg actaacctgc tgtttgtttt
    13741 agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat
    13801 tttgccaaca gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag
    13861 cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg
    13921 gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta
    13981 aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt
    14041 gttcttgtcg tcgatggcgg aggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg
    14101 cgtctggcag tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta
    14161 gatgacctgg aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc
    14221 gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc
    14281 tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat
    14341 attgaatgat aaaaaaggca ccgcaagg
//
LOCUS       DAZFRG010000115        14342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFRG010000115 DAZFRG010000000
VERSION     DAZFRG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.115"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..175)
                     /locus_tag="ACHKUC_002828"
     CDS             complement(<1..175)
                     /locus_tag="ACHKUC_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6619059.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPT"
     gene            complement(302..1237)
                     /gene="alsB"
                     /locus_tag="ACHKUC_002829"
     CDS             complement(302..1237)
                     /gene="alsB"
                     /locus_tag="ACHKUC_002829"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO6619060.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(1296..2186)
                     /gene="alsR"
                     /locus_tag="ACHKUC_002830"
     CDS             complement(1296..2186)
                     /gene="alsR"
                     /locus_tag="ACHKUC_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO6619061.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            2545..2994
                     /gene="rpiB"
                     /locus_tag="ACHKUC_002831"
                     /gene_synonym="alsI"
     CDS             2545..2994
                     /gene="rpiB"
                     /locus_tag="ACHKUC_002831"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO6619062.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            3063..3392
                     /gene="yjdP"
                     /locus_tag="ACHKUC_002832"
     CDS             3063..3392
                     /gene="yjdP"
                     /locus_tag="ACHKUC_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO6619063.1"
                     /translation="MKRFPLFLLFTLFTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(3427..4185)
                     /gene="phnP"
                     /locus_tag="ACHKUC_002833"
     CDS             complement(3427..4185)
                     /gene="phnP"
                     /locus_tag="ACHKUC_002833"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO6619064.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHINHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(4187..4621)
                     /gene="phnO"
                     /locus_tag="ACHKUC_002834"
     CDS             complement(4187..4621)
                     /gene="phnO"
                     /locus_tag="ACHKUC_002834"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO6619065.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(4608..5165)
                     /gene="phnN"
                     /locus_tag="ACHKUC_002835"
     CDS             complement(4608..5165)
                     /gene="phnN"
                     /locus_tag="ACHKUC_002835"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO6619066.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLSLIHQKEKHHACL"
     gene            complement(5165..6301)
                     /gene="phnM"
                     /locus_tag="ACHKUC_002836"
     CDS             complement(5165..6301)
                     /gene="phnM"
                     /locus_tag="ACHKUC_002836"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO6619067.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(6298..6978)
                     /gene="phnL"
                     /locus_tag="ACHKUC_002837"
     CDS             complement(6298..6978)
                     /gene="phnL"
                     /locus_tag="ACHKUC_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO6619068.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            complement(7089..7847)
                     /gene="phnK"
                     /locus_tag="ACHKUC_002838"
     CDS             complement(7089..7847)
                     /gene="phnK"
                     /locus_tag="ACHKUC_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO6619069.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWSVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(7844..8689)
                     /gene="phnJ"
                     /locus_tag="ACHKUC_002839"
     CDS             complement(7844..8689)
                     /gene="phnJ"
                     /locus_tag="ACHKUC_002839"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO6619070.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRHFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGKRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            complement(8682..9746)
                     /gene="phnI"
                     /locus_tag="ACHKUC_002840"
     CDS             complement(8682..9746)
                     /gene="phnI"
                     /locus_tag="ACHKUC_002840"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO6619071.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGDQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQLPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(9746..10330)
                     /gene="phnH"
                     /locus_tag="ACHKUC_002841"
     CDS             complement(9746..10330)
                     /gene="phnH"
                     /locus_tag="ACHKUC_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO6619072.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(10327..10779)
                     /gene="phnG"
                     /locus_tag="ACHKUC_002842"
     CDS             complement(10327..10779)
                     /gene="phnG"
                     /locus_tag="ACHKUC_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO6619073.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(10780..11505)
                     /gene="phnF"
                     /locus_tag="ACHKUC_002843"
     CDS             complement(10780..11505)
                     /gene="phnF"
                     /locus_tag="ACHKUC_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO6619074.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEHQLAVR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ENSPAEYSVSLTRADMIELTMEH"
     gene            complement(11526..12305)
                     /gene="phnE"
                     /locus_tag="ACHKUC_002844"
     CDS             complement(11526..12305)
                     /gene="phnE"
                     /locus_tag="ACHKUC_002844"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO6619075.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(12411..13427)
                     /gene="phnD"
                     /locus_tag="ACHKUC_002845"
     CDS             complement(12411..13427)
                     /gene="phnD"
                     /locus_tag="ACHKUC_002845"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6619076.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKYLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(13452..14240)
                     /gene="phnC"
                     /locus_tag="ACHKUC_002846"
     CDS             complement(13452..14240)
                     /gene="phnC"
                     /locus_tag="ACHKUC_002846"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO6619077.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
BASE COUNT         2967 a         3932 c         4146 g         3297 t
ORIGIN      
        1 tggtcggctc atgtatcccg gataacactt tcattagcgt agatttaccc gcaccatttt
       61 ctcctagtaa tgcatgtatt tcaccaggat aaaccgttaa attaaccgac tttaatgcgt
      121 gaaccggacc aaaggacttg ccgatccccg ccatcgatat atatggcgtg gccataaata
      181 acctctctgc acgaaatatc gtattattgc ctgatggcta cgtttatcag gtctacgttc
      241 aggttatttt gttggccgga taagacactt cattagcatc atcatccggc atcattcagt
      301 tttattgagt gaccaggatt gaatcgacca gtttaaattc cggtgcttta tccagcggga
      361 taaccttgcc ggatttctca gcgtcaacca tcagcttcag acccgttgca ccgatatccg
      421 ccgggttctg ggcaaccgtc gcggtcattt gtccggcttc caccattttg cgggcttccg
      481 gaattccatc tgtaccgacg accaacactt tgcccgtttt tccggcgttt gcgactgcct
      541 gagcaacacc cattgccatc gtgtcattcg cgcaatagat cgctttaata ttcggattac
      601 gttgcaacac gttagtggcg acatccagtg ctttaatgcg gtcccagtcg gcaggctggc
      661 tggcgacaag cttgatctgg cttgcttttt tgaaggcttc ggtggcacca ttacgacgcg
      721 cttcaccgga ggcgttaccg gctttaccct caatgattgc gacttcacca ccttcagcgc
      781 ccaatttatc aataatgaac gacgcgcctt tcgccccgac agcaacgtta tcggtggtga
      841 caaaaccttc cacattgccg ccagcttttt tcagattatc catgtcgatt ttttcatcga
      901 gattaaccag ataaatgcct tttttccatg cgcgggcgac aggcatgacc agattcactg
      961 aggataatgg cgcgaaggcg atacctttgt aatttttatt actgagatct tcaaataact
     1021 gcaattgaga ttgaaaatcg ccttctgaag gagaggcaaa aatatcaacg ctgacgccca
     1081 gcgtttttgc ttcatcttca atgccttttt tcatatctac ccaaaatggg ttggagagtg
     1141 ttttcaatac gacagcatat tcggcggcag caaaagcgct ggttgacaac attaagccca
     1201 cgagtgtgcc gctgaaatat ttcagatatt tattcataat catgttctca cggtaagggg
     1261 agaggtaact ccgcgatggc ggaattacct ttttcttatt tcagcgcgcc tggtgagaag
     1321 aaatcaacaa ttgcgccggt tttttgcata ttaatattag cttgttcaat gttgagctgg
     1381 gcgacagaga caaaaaacgc gtccagcaaa gtcagttgta atattcttgc cgaggcatta
     1441 cgacctaata acggtgtttc cggggccggt gagcaaataa tataatcggc cagcttcgct
     1501 attggtgaat gatagctatg cgttatacaa ataatctttg ccccgttctt ttttgccagt
     1561 tctacagccg cttttacatc actggttcgc ccggaatgag tcaccaccag caccacatct
     1621 ccttcctgta acaacgaagc agacatcatc atgatgtgag cgtccggata ggcctggcag
     1681 cgtacgccaa tgcgtaaaaa cttgtgctgt acatcagcac agatagcatt tgatcctccg
     1741 gcaccgtaca aatcccgctg tctggcctga tagaaaaagc gggcggcacg gtggatctca
     1801 tcaacgttga cgatcgactg accttccata atggtgcgta aagtgatgtt aaatacctta
     1861 ttcaccacat cctgcggcgc ttcatcaaaa gccaactcgg acggcaatac ctgttctgac
     1921 tgagaaaaat aatcttccag cgcactgcgt aagttacgaa agccgctaaa ccccagcagc
     1981 tttgatacct taacgatcat cgcttcagat accgccagag cttctgcgac atctttaatt
     2041 gcgggtgcac aactcaggtt accgggtttg agtaaccact ccacgatgcg gctttcgttt
     2101 tctgtcattc cttcctgctt cattcgcagg taaggcgcta acccgatacc gttcggaagc
     2161 gctgaatcaa actctgactg gctcatcacg ttctcttgtc cttgaattga agacgccact
     2221 atatcaccac agcaagaaca gaaaagatgc tggcaacttc acaaaaaaat aaaatttata
     2281 aagtttgttt tgttgtgaat aatggagagt ggcttaaaat ttcatttcat ttaatcactt
     2341 taaaaacatg atgttatttg tttttctggt ttattggatg atagatgttg ctggatagtg
     2401 agtgtcttta acgctattgc gctgccagat gtaactctgt aaaacagatc gggaaggcgg
     2461 atttcatcga ttttgggtgt ttttaattca tttacatcac aaatgttttt tgattgtgaa
     2521 gttttgcacg ggcggggaag atgaatgaaa aagattgcat ttggctgtga tcatgtcggt
     2581 ttcattttaa aacatgaaat agtggcacat ttagttgagc gtggcgttga agtgattgat
     2641 aaaggaacct ggtcgtcaga gcgtactgat tatccgcatt acgccagtca agtcgcactg
     2701 gccgttgctg gcggagaggc tgatggcggg attttgattt gtggcactgg cgtcggtatt
     2761 tcgatagcgg cgaacaagtt tgccggaatt cgcgcggtcg tttgtagcga accttattcc
     2821 gcgcaacttt cgcggcagca taacgacacc aacgtgctgg cttttggttc acgagtagtt
     2881 ggcctcgaac tggcaaaaat gattgtcgat gcgtggctgg gcgcacagta cgaaggcggt
     2941 cgtcatcaac aacgcgtgga ggcgattacg gcaatagagc agcggagaaa ttgagattca
     3001 tccactactt gcatggatga gtaatgacta atgtggatag agtttctttt tgaggttggc
     3061 taatgaaacg ctttccgcta tttcttttat tcaccctgtt cacgctgtcc accgttccgg
     3121 cccaggccga tattatcgat gacaccattg gcaatattca gcaggcgatt aacgacgctt
     3181 ataaccctga ccgtgggcga gactatgaag attcgcgcga cgacggctgg cagcgtgaag
     3241 tgaacgacga tcggcgcaga caatatgatg accgccgccg ccagtttgaa gatcgccgca
     3301 ggcagctgga cgatcgccag cgccagctgg atcaggagcg tcggcaactg gaagatgaag
     3361 agcggagaat ggaagatgag tatgggcgtt gatgttgagt ttagagcagg catcgcccct
     3421 cacccatcac gccaccccaa tctccatccc atcaaacccc gcctcaaacc ccgacggcag
     3481 tgcgttttcc atcagccacg catcaaactg gtggttgata tgggtcagaa tcacccgtgg
     3541 cgagcggata acctgattca gcgcaagcac ggtatttaaa tcacagtgat tacgcggtgc
     3601 atcctcgcgc ggcgggtggc tgcaatcaat caccattacc tgcggctgat tattgcgtaa
     3661 aaatttcagc gttttttccg gcaagccagc ggtgtcagac agccacgcca cccggctgtg
     3721 tgccgtttcc agcagataac cgaaggtcag ttttgagtgg ttgagcggca ggggcgtgac
     3781 ctgtaacccc tgtaaatcaa acaccacaaa cggttccatc gtgtggctga aatcaagcag
     3841 gcccggatgt ttaaacagat cgtcgcagcc ctgttcatcc ggcgggccgt agaccgggat
     3901 ctgatcgccc acgccccagc gcagcggaaa cagcccctgg acgtgatcca tatgataatg
     3961 cgtcagcaaa aactgctgga acgatcctgg cgaccagcga tcggcgagat cgtgcagccc
     4021 ggcgtcgatc agggtgattg cgtcgttaaa ctttactacg ccgctgcatg gctggcggcg
     4081 atactgcggc gagcgtcgtg ctctggcgca ggccgcgcac tcgcagcccc atgccggaac
     4141 gccctgtgcg ccgccggtgc cggtgagcgt gagggtcagg ctcatgttac agcgccttgg
     4201 tgaagcggaa gtggctctgc tcgtagcctt cgcgcagata gaaacggtgc gcgtcgtggc
     4261 gcttgacgtt ggtggaaagc tcggtcattt ctgccccggc ctggcgggct tcttcttctg
     4321 cccatgccag taatttactg ccgatgttca gaccgcgcgc ctgcggcatc accaccagtt
     4381 cctgaatttc gccgatccag ttgacatgat gcagatgaaa ctgcaaatgc aggccgatca
     4441 tgccgacaac ctcgccatcc aacagcgcca gatggtagcg catattcggg tcacgcaggt
     4501 tggcgttaaa gcccacgcga aacgcctggt ggtcaaactc cgcttgtttt agctcacaaa
     4561 tcagcgcgta aaccgcgtcg gtgtcgtact gcgttgccgg gcgaagttca caagcaggca
     4621 tggtgtttct ccttctgatg aatcagcgac agcagcgtgt cgaccgactg gcgcaggctg
     4681 ccgtcattat tgagtgtatg gcaatcctga ggagtatagc gggcggcgcg cgccagccgg
     4741 gcattaattt cactggcgtt ttcgcggcca cggttttcca ggcgctggcg caggatctcc
     4801 ggcgaaacct gtaaacagat gggcagcagc gccgattgat aacgcgcccg cgcctgcggc
     4861 aggtgggcgc gtgagccgtt gaccaccacg tcaaatccag cgtgcagcca gaggtcgatc
     4921 tcgacgccga cgccataata cagaccgtta gcgtgccagc ttaaggccag aaggttctgc
     4981 cccgcgcggg taaaaaactc ctgctcgctc agggcgatat ggttttcact tccggcgctg
     5041 gcggcgcgcg tgatgtagcg atgcgctacc agtagctgag tttgttcccg ctggcggagt
     5101 tccgtcaaca gactgtcttt tccggagcca gaggccccca ttaaccaaat cagttttccc
     5161 gtcatcagaa caccctttta ccctgacgcc agacgtggtc gatatgaatg tggttgccct
     5221 tgcgatgcgc cagcaccagg tcggcgcgtt tgccctcgcc aatcaccccg cgatcctgaa
     5281 gattaagcgc ctgcgccgga tttttagtca ccagcttcac cgcctgcggc agcgtaaagc
     5341 ggttgctctc gtcatcggcg acgcgaaatg ccgcatcgag caggctggcg gggtagtagt
     5401 cggaagagag gatatccagc aggccaagct gcgccagttc actggccgcc acgttgccgg
     5461 agtgcgaacc gccgcgcacg atattcggcg cgcccatcag cacgttcatg ccatgcttgc
     5521 gcgaggcttc tgccgcttcg aacgtggtgg gaaattcggc gatcacgctg ccaagctgat
     5581 gggattgggc tacgtgggcg tgcgtggcgt catcgtggct ggcgagcgca atgtgtcgcg
     5641 cgcggcacat tgcggcgata gtttcgcgat tcggctgtga ccagcgtgcg gcgagcgcca
     5701 gttgctcctc ttcgtactgt tgcatctgcg catcggtgag cgagtatttg ccctgataat
     5761 attcacgata tttttcgcgg ttggcgaact ggcgctggcc cggtgagtga tccatcagcg
     5821 acaccagcgt taccggttcg cgctgtacca gtttttcaaa cagcggcagc gtggtgtgat
     5881 gcggcagttc gcagcgtagg tgcagacggt gctcggcacg gttgacgccg cgtttctgcg
     5941 tctcttcgat ggcgttgatc atcttctcca gattctccag ccgatcgccg ccgtcgcgta
     6001 catcaccaat cgccacggcg tccagcacgg tggtgatgcc gctcgccacc atcagcgcgt
     6061 cgtggctgct catcgccgag tgggcaggcc agtcaacttt cgggcgcggg gtgaagaatt
     6121 tatccagatt atcggtatgc agctcaatca gccccggtag cagccagccg ccttcgccgt
     6181 ccattgcttc tgacaggtga ctctggcttt cggcaaaggc gcggatttcg ccgtcctgca
     6241 tctccagaga accgtgtacc acctcgtttt ccagcaccag tttgacgtta ttgataatca
     6301 tgaagagact cccattgggt gcaggcggtc ggcgacttgc tcgcgcacgg tttcgtcatg
     6361 gaagataccg acgatggccg cgccgcgggc tttggcttcg cggatcagtt ccactacggc
     6421 ggcgctgttt ttagcgtcca gcgaggcggt aggttcgtcg agcagcagaa tggggtagtc
     6481 gacgataaag ccacgggcga tgttgacacg ctgctgttcg ccaccggaga aggtcgatgg
     6541 cgcgaggtgc cacaggcgtt caggcacgtt caggcgggtg aggagacgcg cggctttagc
     6601 ggcgcaggct tcacgcggta cgccggtatc gagcagcggc tgcatcacca cttccagtgc
     6661 cgaaatacgt gggatgacgc gcagaaactg gcttacccag ccgacagtgg ttttgcggat
     6721 ctccaccact ttgcgcgctg gcgcggtgac caggtcgacc cattcatcac cgtgtttgat
     6781 ctggatttgc ccttcgtcgg gtagatagtt ggcatacagc gagcgtagca gagttgattt
     6841 tccactgccg gaatggccgt ggagtaccac gcattcgccg gcgttgacgg tgagtgaggc
     6901 gccttgtaat acaggcaggc gcacgccgtt ttgctggtgc aggatgaagg ttttactgac
     6961 gttttgtacg ttaatcattt ttcgcctcgt ggttcgggtg tcggatgcga cgctgatgcg
     7021 tcttatccga cctacgggga gcgcatttgt aggccggata aggcgtttac gccgcatccg
     7081 gcatcagctc aattctgcaa aaccgatgac accagcaact gggtatatgg atggtgaggg
     7141 tcgtcgagca cgcggtcggt taacccactc tccaccactt gcccctgttt catcaccagc
     7201 aaacggtccg ccagcaggcg ggcgacgccc agatcgtggg taacaatcac taccgccagg
     7261 ttcagctcca ccaccagacc gcgcagcaga tcgagcaggc gcgcctgtac cgatacatcc
     7321 agcccgccgg ttggctcgtc cataaacacc agcttcggat gcgtcaccag gttgcgggca
     7381 atctgcaaac gctgctgcat accgccggaa aaggtggtcg gcaggtcgtc gatccggttg
     7441 gcgggaatct ccacctcttc cagccacttc tgcgcggtgg cacgaatatc gccgtaatga
     7501 cgcgccccgg ttgccatcaa ccgttcgccg atattgccgc ctgccgacac ctggcggcgc
     7561 aggccgtcaa gcggatgctg atgcaccaca ctccattcgg tgcgcagcag gcgacggcgg
     7621 tcggcctcgc tcatcgcgta cagcgaacgg ttctcgtagc gaatttcccc ttgctgtggt
     7681 gtcaggcggg cggaaattga tttcagtagc gtggtcttcc cggaaccgga ctccccaaca
     7741 atgcccagaa cttctcctgg ccataaatca aaagagacat cgctgaagcc tttgcccggg
     7801 gcataaaggt gggtcaggtt attgaccgaa agtaacggtt gattcattgg cttttggcct
     7861 cgttctgttg gcggcaataa tcggtatcgg agcagacaaa catgcgtttt ccggcgtcat
     7921 ccagcaccac ttcatcaaga tagctgtggg tcgatccgca gatggcgcat ggttcatccc
     7981 actgctggac ggtgaacgga tgatcatcga aatcgaggct ttccacgcgg gtaaacggtg
     8041 gcacggcata gatgcgcttc tcgcgccccg cgccaaacag ttgcagggcg ggcatcatgt
     8101 ccattttcgg gttatcgaat ttcgggatcg gcgacgggtc cattacgtag cgcccgttca
     8161 ccttcaccgg ataggcgtag gtggtggcga tatgaccgaa gcgggcgata tcttcataca
     8221 gtttcacctg catcacgccg tactcctcca gcgcgtgcat ggtgcgcgtt tccgtctcac
     8281 gcggctcaat aaagcgcagc ggttcgggga tcggcacctg gaagataact atctgatctt
     8341 cggtcagcgg cgtttcgggt atgcggtgac gcgtctggat aagtgtcgca tcgtccgtgc
     8401 gctcggtggt gttcaccccg gtcacgcgct taaagaagtg acgaatcgac acggcgttgg
     8461 tggtgtcgtc tgcgccctgg tcaatcacct tcaacacgtc gctttcgcca atcacgctgg
     8521 cggtgagctg aatgccgccg gttccccagc cgtagggcat cggcatctcg cgtccgccaa
     8581 acggcacctg ataaccgggg attgccaccg cttttaagat ggcgcggcgg atcatgcgtt
     8641 tggtctgctc gtcgaggtag gcaaagttgt agccgctcag attagccatg gttctgctcc
     8701 tgttgcagac gtttgagtag ctccagttcg gcctggaaat cgacgtagtg cgggagtttg
     8761 aggtgcgaga caaagcctgc ggcttcgacg ttgtcggcat gcgccagcac gaactcttca
     8821 tcctgcgccg ggcctgtcgc gtgctcgccg tactccgggg cttgtagagc gcggtcaacc
     8881 agcgccatcg ccatcgcttt gcgctcgctc atgccgaaca ccagcccgta gccgcgcgtg
     8941 aagtgcggcg gctcatccgg cgggtcgata aaaccgttga ccatttcaca ttcggtcatc
     9001 agcagttcgc cgacgttcac cgcaaatccc agctcttccg gcacaatcga cacgtcgata
     9061 tagccgctgc ggatttcgcc tgcgaacgga tgattgcgtc cgtaaccacg ctgggtggag
     9121 taggccagcg ccagcaaata gccttcgtcg ccgcgcatta attgttgcag gcgggaggag
     9181 cgtgagcagg ggtaaaccgg cggcgtgcgg gtgatgtcgt ccggctgtgc gccgctatct
     9241 tcttcaaact tcgccagccc ctgacgcgcc agcaggctga aaacgtgcgg cgacggcagt
     9301 tgttcgctgt cggctgtggt cagcgtcggc gtttcgccgt ttgccagcag ggtgaaatcg
     9361 agcaggcgat gggtgtagtc gtaggtgggg ccaagcagtt ggtcgccggg aatgtcttta
     9421 taaattgctg aaatacgccg ttcgagacgc atcccggtgg tgtcgagcgg ctcactgacc
     9481 gccagcttcg ccagcgtggt gcggtaggcg cgcaacagga aaatcgcttc gacgttatcg
     9541 ccgctggcct gtttcagcgc cagcgcggcc agctcgcggt cggcaatacc gccttcggtc
     9601 atcacgcgat ctaccgcgag gttaagctgc tgttcaatct gggcgacgct cagttcaggc
     9661 aaatcagtat cgccccggcg tcggctctct tgcagggcgt gggcggcgtc gatcgccttt
     9721 tcgccccctt tcacggcaac gtacatcagc acacctccac atgagtggtt cgcggaatag
     9781 ccagcaggcg ctcgccgcag gtcaggatca ggtcgatgcc gagcgggaac gggtgcgggc
     9841 gctcggtaag ttcatgaaga atgcactttg gcagttgcgg ggcgatcatc cgttcttcgg
     9901 caatacctgc gccggtgagg cgcaacatac gcccgccgct caggctggtg acctgtaaaa
     9961 tcagcgttgc gcccgcttct ggtgcaacgg cagtgccggt ggaaagggcg ttgagctgct
    10021 cgctggagat cgcctcatct gttaccgcga aggtcgcctg ttccggctga ttaaccagcg
    10081 acgcgttggt atgaaaacgc aggctctgat tgacgatatc gttgctcaat ggcgcggcaa
    10141 gccacaccgg cgtgtcgtta tcggccagcg tcagcagcac gctggtggtg gcgatattca
    10201 gcggctgcca gccgcgcttg agctgatgca gggcgacaat cacgcccggc tcgctcatgg
    10261 cctttaacag gcgacgaaaa ctgtgctggg catcctgcac gggaagcata aaagcggttt
    10321 ccagggtcat gcgttgtctc cgcgaaccat cgtaaagaag tcgacccggc tggcgttcac
    10381 ttcggcctga cgcgcggcaa tgcgtgccat acggtcagcg tccagcgggg cgataagggt
    10441 ttctgataag ttttgaaagt ggcggttttg ctgcatcagc gcatcaatca gcgcacaacg
    10501 ttcggcgtgc tgtttgtcgc gccccagcac ccagctatag ccgagcgtgc cgtcagtcag
    10561 acgcacggcg gcgcgggtga gcgtggcgtc gccagcaaaa aagcgttcgc cggtgccgcc
    10621 cattcgcgcc tgaatctgca ccaggccagt ttcagcggcg cggatcacct cataatcggc
    10681 gttgatgttc agcgcgttca ggcgggctgc cagttcagcc ggttggctgt gcgccagcac
    10741 ggacatccag tgctggcggg tcgcggtatc tgcgtgcatt cagtgctcca tagtgagttc
    10801 aatcatgtcg gcgcgcgtca ggctgacgga gtactccgcc ggactgtttt caccgtcacg
    10861 gtggttaagg gtgcgcacgc acagcagcgg cgacatattc gggatttcaa gacgctggca
    10921 ctctttggcc tgggcgcggc gggcgctgat ccgcgtctgg ctgcggcgta gcgtaatccc
    10981 cgtttgctcg cgcagaaaat cgtgcagcga accgctgtca aagcattgaa gcgttggcca
    11041 gagcgtgaga tctgcgaagt agtggtcgat taaacagagc gcaacgccgt tgacccggcg
    11101 cagggtgcgc aggtggataa cgttctcgcc ctcggcgatc cccagcgcgt cagcgacgtg
    11161 accggaggcg gggcgcagta ccgacagcag tttttcgctg gtgggatggc tgccctggtc
    11221 cagcaggttc tggctaaaac gcgcctgggc gttgagcggg taatcgaacg ggcgcatcag
    11281 caccagcacg ccgacgccct gacggcgctg tacccagcct ttttccacca gttggtcgat
    11341 ggcgcggcgc agggtgtggc ggttcacctc aaagcgcacc gccagttggt gttcggcggg
    11401 aagataatcg ccacagcggt aatgctgacg aagctcctgc tcaagttttg cggctatctc
    11461 ctgatagcgt gttgggtagc tggtcggatg tgtagacaag tgcatagcca tcaatgcctc
    11521 gctaatcaga taaagtgctt acgcaaccgt tgagagagga aatccagcag gctgaccgtg
    11581 acgataatca gcaccatcag ggcgcaggtt tgctggaact ggaaaccgcg aatcgcttcc
    11641 cacagcgtga cgccgatccc gcccgcgcca accataccga cgacggtcgc cgagcggacg
    11701 ttggattcga agcgatagag ggagtaggaa atcagcagcg gcatgacctg cggtagtacg
    11761 ccgtagagga tctcttccag cttgttggct ccggtagcgc gaatgccctc caccgggccg
    11821 ggttcaatcg cttctaccgc ttcggaaagc agtttggaaa gtacgccggt ggtgtggata
    11881 aacagcgcca gcacgccagc gaacgggccg aggccgacgg cgaccacgaa cagcatggcg
    11941 aagaccattt cgttaatggc gcggcaggcg tccatcaggc ggcgtacagg ttggtagacc
    12001 caccacggta ccaggttttc agcgctcatc aggccgaagg ggatggagag aaccaccgcc
    12061 agcgcggtgc cccagacggc gatttgcagc gtgacggcca tttcggtgag gtaatcctgc
    12121 cactggctga aatcgggcgg gaagaagtcg gcggcgaacg tcgccatgtt gccgccatct
    12181 ttaatcagcg taagcggggc catttccgcg ccctgccacg agacgaccag cacggcgata
    12241 acaacggccc agctcagaag cgagaaccag ctgcgcttgg gtggggcgat ggtgatggtt
    12301 tgcatgtttg gctccggtct gtaggccgga taaggtgctt gcaccgcatc cggcatcaac
    12361 gtctgcacat tgtcggatgc gacgccgggg cgtcttatcc gacctacgat ttactgcacc
    12421 gctttactca ccgaactcat tgcgcttaac gcgttgttca ggcggtccaa atcatccagt
    12481 tgcgcctgaa tggcggtggt ttttgccagc ttgtcctgtt cgttcagccc tttgttgtcc
    12541 ttcacgcttt gcatctcttt aaacagtgcg agctggcgaa tcggcaccag ttgcaagtcg
    12601 ctggaggcgc ggaacggcgc ccagcccagg cgctccagca ccgctttctc ttccggcgtt
    12661 ttgccgtaat tcataaagaa gtcgtagatc ttgtctttag tggtttcgga aagatttttg
    12721 cgccagacga tcggatcgcc agggatcagc ggtgatttcc agatcacctt cagttctttc
    12781 agcttctccg gcgcggaggt tttcagcttg tcgaggtttt cggtgttgtt ggtggcgaca
    12841 tccacttgct tgttagcgac ggcgagggcg ttggtttcat gcccggcgtt gacggtgcgc
    12901 ttaaagtcgc tggcggagat attgttttta gcgaagacgt agtaaccggg gacgaggaag
    12961 ccggaggtag agttaggatc gccattgccg aaggtgagat atttccgctt cgccagcaga
    13021 tcgttcaggt tgttgatcgg gctatctttg ttgacgatca acacgctcca gtaacccggc
    13081 gatccatccg ccgcgaccgt ctgggcgaag acctgaccgt tggcgcgatc caccgcttcc
    13141 atcgccgaca ggttgccgta ccaggcgata tccactttat tgaagcgcat cccctggata
    13201 atgcccgcgt agtccggggc aaagaaggcg ttgaccttta cgcccagctt cttctccata
    13261 tcctgtaaga acggcgtcca ttgcggtttc aggttttgct gtgattccgt tgaaataatg
    13321 ccgaaattca gcgccttttc ctgctcttcg gcgtatgccg gatttaacag ggtgctgagg
    13381 ctgaacatgc tggtgaaggc cagcgaggca attatcttag cgttcattcg ttttcctcaa
    13441 tgatggggat gtcaggcagc tttcgcgttc tcttcgacgc ggttaatgct gcggtagaga
    13501 tggtcaaaac gttcgttatc aaactgttgg ctgcagccgt cgtagaagac gtgcccctgg
    13561 cgcagggcga cgatgcgttc gcagtagcgc agggcgtaat ccacctgatg cagcgtgacg
    13621 accacggtaa tgccgtcgtt ctggttgatg tcgcgcaggg tgtccatcac gatgcgtgcc
    13681 gattccgggt ccagcgaggc gatgggttcg tcagcgagaa tgaccttcgc ctgctgcatc
    13741 agcgcgcggg caatcgccac acgctgttgc tggccgccgg agagtgtgga aacgcgttga
    13801 tgggcaaagt gcaccatgcc aacgcgggtc agcgcctgta acgcgcgctg tttctgctcg
    13861 cgggtgaagt agctaaaaca ggttcgccag aatggcgtgc tgccgagcgc gccaatcagc
    13921 acgttctcca gtacgctcag gcgattcacc aggttgaatt gttgaaagat gtagccggta
    13981 tgggcgcggc ttttgcggat atcgcgggcc agacggcctt cgcgctggac agtgcggccc
    14041 agcagctcga tatggctacc gacagattta tcgccggtaa tcaaaccgct taagtgacgt
    14101 aaaagagtgg attttccgga acccgacggc ccaagcagag ccaccatttc accatgatga
    14161 atgttcagat caaccgcgtg cagcgcctga tgctgattga aggttttggc gagcttctcg
    14221 acacggataa tcgtttgcat gatgcagcct ccctaaaaaa gtggctccat cgtggcggat
    14281 tattgtgacg atttggttaa ttaaaagtga ctaacagatg aagagttaac gggaattcga
    14341 tg
//
LOCUS       DAZFRG010000116        14326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFRG010000116 DAZFRG010000000
VERSION     DAZFRG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.116"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(243..1892)
                     /gene="pgi"
                     /locus_tag="ACHKUC_002847"
     CDS             complement(243..1892)
                     /gene="pgi"
                     /locus_tag="ACHKUC_002847"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO6619078.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2129..2325
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2417..3766
                     /gene="lysC"
                     /locus_tag="ACHKUC_002848"
     CDS             2417..3766
                     /gene="lysC"
                     /locus_tag="ACHKUC_002848"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO6619079.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4019..4291
                     /locus_tag="ACHKUC_002849"
     CDS             4019..4291
                     /locus_tag="ACHKUC_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO6619080.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4292..5164)
                     /gene="rluF"
                     /locus_tag="ACHKUC_002850"
     CDS             complement(4292..5164)
                     /gene="rluF"
                     /locus_tag="ACHKUC_002850"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO6619081.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5512..6459
                     /locus_tag="ACHKUC_002851"
     CDS             5512..6459
                     /locus_tag="ACHKUC_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6619082.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDIQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6529..7335
                     /locus_tag="ACHKUC_002852"
     CDS             6529..7335
                     /locus_tag="ACHKUC_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6619083.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNSIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7345..7752
                     /locus_tag="ACHKUC_002853"
     CDS             7345..7752
                     /locus_tag="ACHKUC_002853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO6619084.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7752..8246
                     /locus_tag="ACHKUC_002854"
     CDS             7752..8246
                     /locus_tag="ACHKUC_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6619085.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8312..9109
                     /locus_tag="ACHKUC_002855"
     CDS             8312..9109
                     /locus_tag="ACHKUC_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO6619086.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9120..9944
                     /locus_tag="ACHKUC_002856"
     CDS             9120..9944
                     /locus_tag="ACHKUC_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO6619087.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9996..11225
                     /gene="sorE"
                     /locus_tag="ACHKUC_002857"
     CDS             9996..11225
                     /gene="sorE"
                     /locus_tag="ACHKUC_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO6619088.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLIITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAVILAHHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11606..12295
                     /gene="pepE"
                     /locus_tag="ACHKUC_002858"
     CDS             11606..12295
                     /gene="pepE"
                     /locus_tag="ACHKUC_002858"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO6619089.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12385..14016)
                     /locus_tag="ACHKUC_002859"
     CDS             complement(12385..14016)
                     /locus_tag="ACHKUC_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO6619090.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3488 a         3611 c         3735 g         3492 t
ORIGIN      
        1 ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct gataaaaaat
       61 gccgacataa cgtcggcatt gtttttaagg ctatttaatc aatcgtacta tgtattaatt
      121 gccgaatacg atgtacatat cggcatcgac ctgtaggcct gataagacgc gaccgcgtcg
      181 catcaggcat cggttgccgg atgcggcgtg aacgccttat ccggcctaca tatcgacaat
      241 gattaaccgc gccacgcttt atagcggtta atcagaccat tggtcgagct atcgtggctg
      301 ctgatttctt tgtcatcttt cagctctggc agaatacggt tcgccagctg tttacccagt
      361 tccacgcccc actggtcgaa ggtgaagatg ttcaggatca cgccctgagt aaagattttg
      421 tgctcataca gcgcaatcaa cgcacccagg ctgaacggag tgatttcacg cagcaggatg
      481 gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc
      541 ggatctttac cctgatcacg atattcctgc tcaaccactt cgcgggattt accaaacgcc
      601 agcgcttcgg tctgagcaaa gaagttagac agcagtttct ggtgatgatc agagagcggg
      661 ttatgggtga tagccggagc gatgaaatcg cacggtacca ttttggttcc ctggtggatc
      721 agctgataga acgcgtgctg accgttcgtg ccaggttcac cccagataat cgggccagtc
      781 tggtaatcca caaccttacc gttacggtca acatacttac cgttggattc catattgccc
      841 tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat cgcttcagtt
      901 tccgcgccaa agaaattgtt gtaccagatg ccaatcagcg ccagcagtac aggcaggttt
      961 ttctcggcag gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca
     1021 acgaagttat caaagccgat ggagagaaca atcgacaggc caatcgctga ccacaaagag
     1081 taacgaccgc cgacccagtc ccagaattcg aacatgttgg cagtatcaat accaaactcg
     1141 ccaacggctt tggcgttggt tgagagcgcc gcaaagtgtt tcgcaacgtg cttctcatca
     1201 cctgccgctt tcaggaacca gtcacgcgcg ctgtgggcgt tggtcatggt ttcctgagtg
     1261 gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt tttcagcact
     1321 tccgcgatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg gtttttgtat
     1381 ggacgcagtg cttcagtcac catgtacggg ccgaggtcag aaccgccgat cccgatgttc
     1441 actacgtcag tgattgcctt gccggtatag cctttccact caccggaaat aatcgcttcc
     1501 gagaaggttt tcatcttctc cagcaccgcg ttgacttccg gcataacatc tttgccatca
     1561 accagaatcg gggtattgct acggttacgc agcgctacgt gcagtacggc gcggttttca
     1621 gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag atcgcactct
     1681 ttcgccagat cttgtaattt cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc
     1741 accagcatct gatcgtcgaa ggttgcggag aacttagaaa aacgatcgcc gtctttagca
     1801 aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag tgcctgccag
     1861 gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat tttgagaatt
     1921 gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta gtgccgttag
     1981 cgtaatgttg agtgtaaacc cttagcgcag cgaagcattt attagctgaa ctactgaccg
     2041 ccaggagtgg atgaaaaatc cgcatgaccc catcgttgac aaccgccccg ctcacccttt
     2101 atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg
     2161 gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga ggtgattgaa
     2221 cggctggcta cgttcatcat cggctacagg ggctgaatcc cctgggttgt caccagaagc
     2281 gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata gtagcgaagt
     2341 atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa aatggatccc
     2401 ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc
     2461 tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca acgtacgttt
     2521 agttgtcctc tcggcttctg ctggtatcac taatctactg gtcgctttag ctgaaggact
     2581 ggaacctggc gagcgattcg aaaaactcga cgcaatccgc aatatccagt ttgccattct
     2641 ggaacgtctg cgttacccga acgttatccg tgaagagatt gaacgtctgc tggagaatat
     2701 tactgttctg gcagaagcgg cggcgctggc aacgtctccg gccctgacag atgaactggt
     2761 cagccacggc gagctgatgt cgaccctgct gtttgtcgag atcctgcgcg aacgcgatgt
     2821 tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgatcgat ttggtcgtgc
     2881 agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgcttc cacgcctcaa
     2941 tgaaggctta gtgatcaccc agggatttat cggcagcgaa aataaaggtc gtacaacgac
     3001 gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt tacacgcatc
     3061 tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac gcgtggtttc
     3121 cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg
     3181 tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata tcccggtctt
     3241 tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa
     3301 tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca ctttgcacag
     3361 cctgaatatg ctgcattctc gcggtttcct cgcggaagtt ttcggcatcc tcgcgcggca
     3421 taatatttcg gtagacttaa tcaccacgtc agaagtgagc gtggcattaa cccttgatac
     3481 caccggttca acctccactg gcgatacgtt gctgacgcaa tctctgctga tggagctgtc
     3541 tgcactgtgc cgggtggaag tggaagaggg tctggcgctg gtcgcgttga ttggcaatga
     3601 cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac cgttcaacat
     3661 tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc ccggcgaaga
     3721 tgccgagcag gtggtgcaga agctgcattt taatttattt gagtaaacac agtatggccc
     3781 ggaagacatt atttcgggcc gtattgtttt tcttgtcatt atgcccataa ataaacgagc
     3841 ctgtactctg ttaaccagcg tctttatcgg agaataattg cctttaattt tttatccgca
     3901 tctctaacta attatcgaaa gagataaata gttaagagaa ggcaaaatga atattatcag
     3961 ttctgctcgc aaacgaattc ccgtacactg aactctctgg ctcactgagg gcaccatcat
     4021 ggcactcccc cgtattaccc aaaaagagat gactgaacgc gagcagcgtg aacttaaaac
     4081 actgctggat cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg aaactaacag
     4141 cattaagaaa gagtacatcg ataaattaat ggtcgagcgt gaggctgaag cgaaaaaagc
     4201 ccgccaattg aagaaaaagc aggcttataa accggatcct gaggcgtcat ttagctggtc
     4261 agcaaatacg tcgacgcgcg gcaggcgctg attaacgccc cttctttttg cgccccggcg
     4321 aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt ttttccatct
     4381 taacgactgg acgtttgatg cctaccgttt tcggtttggc cttcgcttta ggttttgcct
     4441 cggaagagga attttcaatg agcttaaaga gatcgattaa ctcatcgtcg gttaaatcgc
     4501 gccactcacc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta cgttccagct
     4561 ttttcacttc gtaaccaaag tgctcgcaca tgcgacgaat ctgacggttc agcccctgca
     4621 ccagggtaat gcggaagaca aacggtgctt cttttttgac tttgcacttt ttagttaccg
     4681 tcccgaggat cggtactccc gcccccatac cacgaataaa ttcatcggta atcggtttat
     4741 caaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc ttattcacca
     4801 gatcgccgtg attggtgagg aaaatcagcc cctgggaatc tttatccagg cggccaatcg
     4861 ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg
     4921 tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct tcggattccc
     4981 gaggctcaat caactgacca tttactttca caacgtcgcc gggtttcacc tgatcgccaa
     5041 tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga tccgcttcgc
     5101 ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat gagtcgggca
     5161 gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga aaaacagggt
     5221 tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc
     5281 ggaagtacga tgcaaatttg cctggcaaaa taagaataat caacaaatta cgcatcgctg
     5341 cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc
     5401 cccccatgca aagagtgaaa aatcgctttg aaaggccatc atgatggggt tatatcagat
     5461 caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac
     5521 agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca ggatatgacg
     5581 caggcgcaaa ttgcgcgcga actgggtatt tatcgcacta ccatcagccg cttgcttaaa
     5641 cgaggccgcg atcagggaat tgtcaccatc gccatcaact atgactacaa cgaaaatctc
     5701 tggctggagc agcaagtgaa gcaaaagttt ggcctgaaag acgttgtggt ggtgtcagga
     5761 aatgatgagg atgaagatat tcaactggcg atgatggggt tacacggcgc gcaactgctg
     5821 gatcgcttgc tggaacctgg cgatattgtc ggtttttcct ggggccgcgc ggtgagcgca
     5881 ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatttgcgt gccgattatt
     5941 ggcggcccgt ccggtaaact cgaaagccgc tatcacgtaa acacattaac ctacagcgcg
     6001 gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggcgctgct ggataaccca
     6061 ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc ctactgggat
     6121 aatctggatg tcgccctggt gggaattggc tcaccggcca ttcgcgacgg cgctaactgg
     6181 catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt tgctggcgat
     6241 atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat gagcgaaaaa
     6301 acactctcta tcgaaatgaa taaattaaag caggcacggt attccattgg cattgccatg
     6361 agcgaagaaa aatacagcgg aattgttggt gcactgcgtg gaaaatatat taattgtctg
     6421 gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg atgtttaccg
     6481 taataaaaat tacggcgggt ccctactatc taaaatcagg agtctgttat gcaaacgtgg
     6541 ttaaatttgc aggataaaat cattattgtc accggtggcg catccggtat tggtctggcg
     6601 attgtagagg aattattagc acaaggcgcg aatgtacaga tggtcgatat tcacggtggc
     6661 gatggtcaat atgaaagcca taaaggttat cagttctggc cgaccgatat ttccagcgcc
     6721 aaagagataa atcatacggt agcagaaatt atccagcgtt ttggtcgcat cgacggtctg
     6781 gttaacaacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg
     6841 cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca gaaaggcgtt
     6901 tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg
     6961 aatgtttcct cagaaagtgg gctggaaggc tcagaaggcc aaagctgtta cgccgcgacc
     7021 aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggcaa gcacggtatc
     7081 cgtgtggtcg gtatcgcgcc agggattctg gaaaaaacag gactgcgcac gccggaatat
     7141 gaagaagcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga aggctatacc
     7201 aaaaattcca ttcctattgg gcgcgccgga agattagcag aagtggctga ttttgtttgt
     7261 tatctgctgt ctgaacgcgc cagctatatc accggagtaa ccactaacat tgcgggcggc
     7321 aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgcgca tggcaaatta
     7381 gcttgcgcca tgctggaatc ggttcagatg gtgtacggcg atgccagggt cgaggccgtt
     7441 gaatttgtgc ccggcgagaa cgccggagac atcgtagcaa agctggaaaa gttagtaagc
     7501 attcacaatc aagatgagtg gctaatcgcc gtggatttac agtgcggcag cccgtggaat
     7561 gcggcagcga tgcttgcgat gcgcaatcct cgtctgcggg taatcagcgg cctttctctg
     7621 ccgctggcgc tcgaactggt tgataaccag gacagcatga atattgatga actgtgtgaa
     7681 cacctgacgc aaatcgcaaa gcaaacctgc gtcgtctgga gacaggtggc aacagcggag
     7741 gaggatttct gatgaacatt acgctcgccc gcattgatga ccgcctgatc cacggccagg
     7801 tcactaccgt ctggtctaaa gtggctaacg cccaacgcat tattatctgc aatgacgaag
     7861 tttataacga tgaagttcgc cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg
     7921 ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa tatcaggacg
     7981 aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcaatggtt cgccaggggg
     8041 taaaaatagg taccctgaat attggcggta tggcctggcg tcctggtaag aaacagttaa
     8101 ccaaagcagt ttcattagat gacgatgata ttaacgcatt tcatgagtta aataatcttg
     8161 gcgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa
     8221 taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc tttaacaggc
     8281 agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac aaatcattgc
     8341 tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat ttcagactca
     8401 tcgtccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa aaaccggaat
     8461 tatgctcggt ggtacgctgg aattgatagc tctcggctgg atgaacgtcg gcgcggcgca
     8521 atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg ttggtcagca
     8581 aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac
     8641 ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag cagcggaaga
     8701 agcacggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc aggcactgcg
     8761 cgttgctatt ccggcactga ttgtctcgct gttcgtcagc gctgatatgg tgagcaatat
     8821 gctgagcgcc attcccgaat ttgtgacccg tggactgcag attgctggcg gttttatcgt
     8881 ggtggtcggt tacgccatgg tgcttcgcat gatgggcgtg aaatatttga tgcctttctt
     8941 tttcctcggt ttcctcgcag gtggctacct cgatctcagt ctgctggcgt tcggtggcgt
     9001 cggcgtgatc atggccctgc tctacatcca gttaaatcca cagtggcgta aagctgaacc
     9061 acatccccag accaccacta tcaccgccct tgaccaactt gatgattaac ggagccatca
     9121 tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc
     9181 aacaggcatc ctttaacttt gaacgtattc acgggctggg tttttgctac gacatgatcc
     9241 ccgccatcaa acgactttac ccattaaaag aggatcaggt tgcggcgctc aggcgacacc
     9301 tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgtc actgccgcca
     9361 tggaagaggc gcgagccaac ggcgcggaaa ttgatgacgg taccattaac ggcatcaaag
     9421 taggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga acgctgcgcc
     9481 cgattaccgc cgcgctcggc gcatctctgg cactttcggg caacattctc ggccccctgc
     9541 tgttcttctt tattttcaac gcggtgcgtc tggcgatgaa gtggtatggc ctacagctcg
     9601 gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg taatttgctg caaaaactca
     9661 ccgaaggcgc gtcgattctc gggctgtttg tgatgggcgt gctggtgacc aaatggacgt
     9721 caatcaacgt accgttggtg gtttcacaaa cgcctgccgc cgatggtgcc accgtcacca
     9781 taaccgtgca gaacatcctc gaccaacttt gccctggttt gctggcgctc ggtctgacgc
     9841 tgctgatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt ttcgccctgt
     9901 ttggtttagg gattatcggt aatgcgctgg gcttcctgtc ctgattcttc gccccggcgc
     9961 aaccgtcggg gccatcgttc aacacgaggt ggtttatgaa aacaacagct ctgcgtcttt
    10021 atggtaaacg tgatttacgc ctggaaacct ttgaccttcc tgaaatgcag gaggatgaaa
    10081 tcctcgcaac ggtggtcact gacagcctgt gcctctcttc ctggaaagag gccaatctgg
    10141 gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa ccccatcatc atcggccacg
    10201 aattttgcgg cgatattctg gccgtgggta aaaagtggca gcacaaattc cagccgggtc
    10261 agcgttatgt gattcaggcc aacctgcaac tccccgaccg cccggattgc cccggctact
    10321 ccttcccgtg ggtaggcggc gaagcgacgc atgtggttat tcccaacgag gtcatggaac
    10381 aagattgcct gctggcatac gacggcgaaa cctattttga aggctcgctg gttgaaccgc
    10441 tttcctgcgt gattggcgcg ttcaacgcca actatcatct tcaggaaggt agttataacc
    10501 acacgatggg gattcgcccg caagggcgca cgctgatcct cggcggcacc ggaccaatgg
    10561 gactgttggc gattgattat gcgctacatg gacccgttaa cccttcgctg ctcatcatta
    10621 ccgataccga caacgacaaa ttgagttatg cgcgcaagca ctatccatca gaaccgcaaa
    10681 cactgattca ttatctcaat gccgccgatg cagcatttga tacgttaatg gcactgagtg
    10741 gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa tgaaggactg gtgactctcg
    10801 cctcttcctt gctggcgaca gatggttgtc tgaatttctt cgccggaccg caggataagc
    10861 acttcagcgc gccaattaat ttctacgatg tgcattatgc atttacccac tacgtgggca
    10921 catcaggcgg caataccgac gacatgcgcg cagcggtcaa attgattgaa gagaaaaaag
    10981 tgcaggccgc aaaagtggta acacatattc ttgggctgaa tgccgcgggc gaaaccacgc
    11041 ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa tacctgccgc
    11101 tgacgtcact cacgcagatt caggatcaag cactggcggt gattctggcg catcatcagg
    11161 ggatctggtc cggtgaggcg gagcaatacc tgctcgccca tgcagaggca atttcccatg
    11221 attaatcgcg ctacttaact ctgcatgtcg ctggctggtc gccgcagcaa cttcgcggcc
    11281 tgatgtgcgg ctgattgcag aagcggcaca atgtgcacga gaagaataaa aaaacatcag
    11341 cgccttaagg ggcgctcaaa ttggtggcaa agtgcacatt gtttatgccg gatacggaat
    11401 gaagccttat tcgcctacat aatcttgaaa attcaatata ttgcagttat ctggtaggcc
    11461 aggtaagcgt agcgcatcag gcaatcctgt gtttgtcatc agtctcagcg cctgtgcaat
    11521 atgtgacatc gtgagccatg tcagtaagtg gttaaattcg cagtggtagc attccttccg
    11581 tgttttctta ctaaccggag aagtaatgga actgctttta ttgagtaact cgacgctgcc
    11641 gggtaaagcc tggctggaac acgcgctgcc gctgattgct gaacagttgc agggtcgccg
    11701 ttctgcggta tttattcctt tcgctggcgt aacgcagacc tgggatgatt acacagcgaa
    11761 aacggctgcg gttctcgctc cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga
    11821 tcccgttgcc gcgattgaaa atgcggaaat tgtgattgtc ggcggcggta atactttcca
    11881 gttgctgaaa cagtgccgcg agcgcgggct gctggcaccc attactgacg tggtgaaacg
    11941 tggcgcgctg tatattggct ggagcgcagg cgctaacctt gcttgcccaa ccattcgtac
    12001 caccaacgat atgccgattg tcgatccgca aggtttcgat gcgctaaatc tgttcccgct
    12061 acaaatcaac ccgcacttca ccaacgcgct gccggaaggc cataaaggtg aaacccgtga
    12121 gcagcgtatt cgcgaactgc tggtcgtcgc gccagaactg acgattattg gtctgccgga
    12181 aggtaactgg atcacagtaa gtaaaggtca cgctacgctg ggtggcccga acaccactta
    12241 tgtgtttaag gctggtgaag aagcggtgcc gctggaagcg ggtcaccgtt tttaagtgaa
    12301 ttgccggatg catgatccgg catcttgttc atagtgcctg atgcgacgct ttacgcgtct
    12361 tatcaggcct tcagggtcac ggtattaata ctcgtcccgt ccttcatctt catccggctg
    12421 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag
    12481 taagcctaaa tgtagcgaac tggtttcaat actctgcacg ttttgctggt gcaggcgatc
    12541 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaaa cgatgtttgc tgcggcgcaa
    12601 acgacgggcg ctggtaacat cgccagagaa gaaaaccgac attgccagtt ttaaattact
    12661 gagcaattgc tcatagagcg catccagttc cttcaagcca tcaagcgaaa atgctcgcct
    12721 tgctgccagc gatttgtcgg caatttcact ccccatgcgc tcgacgatat cggaggcctg
    12781 ttcaaggttg agcgacattt cgatgatctc cgcccagcgg cgcgactctt cttctgccag
    12841 ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa cgttgatgtc
    12901 atcagccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcaa
    12961 cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcggg cagcgttcgc
    13021 cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
    13081 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacgaa
    13141 tggcaacatg accaggcagc gtacaaggtt gtagaagacg tggaaataga tcaccaattc
    13201 cgcttttggc aatggcaact tccccattgt ctctgccagc aaatgaacaa acggcaggat
    13261 aatcaggcta cccaccagct taaacagcag gctacccagc gcgacacggc gggctgcggc
    13321 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggtttg ccccaatcac
    13381 cagacaaagc gccactggga aagagataat gcctgcggcg gtcagagtcg cggtcagcag
    13441 taccgcggca aggctggagt agctgataat ggcgaacatc gcaccaatca gcgcatccag
    13501 caaaatgtcg ccagtcagcg aggcaaagat cacctgaacg ccgtttgcct gagtgatcgg
    13561 cgttacggcc tgcacaatca actccagcgc cagtaaaatc agcccaagac caataccgac
    13621 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
    13681 aataagtaac ggtgacagcc aggataaatc gaaggtgaga atacgcgcca tcagcgccgt
    13741 cccgacatct gcacccagca caatcaccag cgcaggcgcg agagcaacca gatcctgagc
    13801 gacgaacgag gtcaccagca tggtggtggc attactgctc tgcaccagtg cggtaacgcc
    13861 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcag
    13921 acgcgcgccg aagacgcgca ttacgcctgt tcgaacaata tgagtccccc agaccagcag
    13981 ggcgacggca gaaagcaggt gaagcaaagt tagcacggaa tcaggttctc ctttttgtta
    14041 tgcattttcc tgttaagatt tttctaatca cgttgttttt atccaggcat ttctcttgta
    14101 tgtactttca gtataagaga tttatacaag aaagcattta aaaaggcatt tatataaaaa
    14161 tccacctgta tctgttgcta tttctgtcac actttcccct gtcgtttaca ccagataatc
    14221 gttgtcactt ttgtgacctc tgagaagata aacaacgaaa tatttaagac cagatcctgt
    14281 gaataaaaat aattaagtaa caacggtatt acggagattg aacagc
//
LOCUS       DAZFRG010000117        14268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFRG010000117 DAZFRG010000000
VERSION     DAZFRG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.117"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(788..943)
                     /locus_tag="ACHKUC_002860"
                     /pseudo
     CDS             complement(788..943)
                     /locus_tag="ACHKUC_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1186..2208
                     /gene="istA"
                     /locus_tag="ACHKUC_002861"
     CDS             1186..2208
                     /gene="istA"
                     /locus_tag="ACHKUC_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFO6619091.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     DQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            2205..2987
                     /gene="istB"
                     /locus_tag="ACHKUC_002862"
     CDS             2205..2987
                     /gene="istB"
                     /locus_tag="ACHKUC_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO6619092.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTEQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(3054..3182)
                     /locus_tag="ACHKUC_002863"
                     /pseudo
     CDS             complement(3054..3182)
                     /locus_tag="ACHKUC_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(4013..4615)
                     /locus_tag="ACHKUC_002864"
     CDS             complement(4013..4615)
                     /locus_tag="ACHKUC_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619093.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLIHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(4709..4915)
                     /locus_tag="ACHKUC_002865"
     CDS             complement(4709..4915)
                     /locus_tag="ACHKUC_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6619094.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKVGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(5111..6307)
                     /locus_tag="ACHKUC_002866"
     CDS             complement(5111..6307)
                     /locus_tag="ACHKUC_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFO6619095.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLTMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFDSVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            6539..6772
                     /locus_tag="ACHKUC_002867"
     CDS             6539..6772
                     /locus_tag="ACHKUC_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619096.1"
                     /translation="MRKDASARTRDKEGIVFVRTGREAGVLIIAELFSEVVTVSQPVP
                     GEQETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7104..7247
                     /locus_tag="ACHKUC_002868"
                     /pseudo
     CDS             7104..7247
                     /locus_tag="ACHKUC_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(7487..7687)
                     /locus_tag="ACHKUC_002869"
     CDS             complement(7487..7687)
                     /locus_tag="ACHKUC_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619097.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            7887..8456
                     /locus_tag="ACHKUC_002870"
     CDS             7887..8456
                     /locus_tag="ACHKUC_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6619098.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
     gene            8622..9023
                     /locus_tag="ACHKUC_002871"
     CDS             8622..9023
                     /locus_tag="ACHKUC_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6619099.1"
                     /translation="MYAKSFLALDSNGRLTGARTVQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            9011..9421
                     /locus_tag="ACHKUC_002872"
     CDS             9011..9421
                     /locus_tag="ACHKUC_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO6619100.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            9705..11324
                     /locus_tag="ACHKUC_002873"
                     /pseudo
     CDS             9705..11324
                     /locus_tag="ACHKUC_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            11404..11696
                     /locus_tag="ACHKUC_002874"
                     /pseudo
     CDS             11404..11696
                     /locus_tag="ACHKUC_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(11736..12770)
                     /locus_tag="ACHKUC_002875"
     CDS             complement(11736..12770)
                     /locus_tag="ACHKUC_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFO6619101.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(12773..13738)
                     /locus_tag="ACHKUC_002876"
     CDS             complement(12773..13738)
                     /locus_tag="ACHKUC_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6619102.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSKTMKKMEV"
     gene            complement(13792..>14268)
                     /locus_tag="ACHKUC_002877"
     CDS             complement(13792..>14268)
                     /locus_tag="ACHKUC_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO6619103.1"
                     /translation="FYQLRIKPNALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKN
                     TVLADVLPVTMLDGDDRVEKPEGVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAE
                     VVLTINNDPLLVFGNYHNGKIACFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
BASE COUNT         3547 a         3507 c         3739 g         3475 t
ORIGIN      
        1 cagcgcatct gtctccttta agtaaaaaat gttttcacgt attgctgtaa attcatttta
       61 aatcagtgta ttaccttgat attgctgtct cctggtttga tgcgtaatcg ttctgccttt
      121 ctggatgatt ctggtgcttc cgggtgcatt ctcctctgca catgcaacag ttcgtcatcg
      181 ccacgttccc aaaaagcata taaaaacgaa tcgaatttga tatttttttc aatcgttttt
      241 cataggaatt tcgtagggtg ttctccgcta taacctccat atttgctgtc tttttcttca
      301 taaatcgtat gcaatgtagc ctgtctgtca agacctcttc tctgctgttg aggagacgat
      361 ctcatttttg gtcttttccg ggagagaggc tgtcagatga gcgagccgga tttactgtca
      421 gaggtacacc ccgtagcaga cctgtgctcc catttccgcg atccggaacc aacaacgccc
      481 tacggttaat actatttgaa gcgtcaggta ctgcaggaag ttcccccctg cgcggacatc
      541 ggttcctgcc gacatctcac aatacggtca gactgtcatg cagtaacacc cgcaatgcgg
      601 tctgcggttt taagtgcacc gataatgcgt catagtaagt ctgggggagt tacggaatgc
      661 ctgttaagtc gtgccgtggg tggagacagc ttacttttcc acagcaactg cagtcattca
      721 ggatgatcag gagatattct gtcaatgtca cgatcctgcc attttcgtac ccgctaagat
      781 acttccgtta ctggtgctgt ccgcttttca ccatttcatc ctcactgaca ggcgttgcgt
      841 tttgggtcat caccacagct ttacgtttct cttttaaccg gtaacgctct cctttgatat
      901 tcagcgtggt tgagtgatgc agcagtcaat ccaggagctc tgttgtccgg caaccaccat
      961 atcaacttct tcccgggtgt ggcggagagg tggtatcctg gctgagtttc gaagcgaacc
     1021 gttctttttg acgggcgcat tttacgtccg ggctgaatgt agtaacgcgg catggaacgt
     1081 cggcccgtat ggcccattgt gtcacgaacg gtgcaatagt gatccacacc caacgcctga
     1141 aatcagatcc agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag
     1201 acagttatgg aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga
     1261 gaactgggga tctcccgcaa tacggttaaa cgttatttgc aggcaaaatc tgagccgcca
     1321 aaatatacgc cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt
     1381 caacgcatcg ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc
     1441 agagaccagg gatatcgtgg cggaatgacc attctcaggg gattcattcg ttctctctcg
     1501 gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag
     1561 gttgactggg gcactatgcg taatggccgc tcaccgcttc acgtgttcgt tgctgttctc
     1621 ggatacagcc gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggaa
     1681 acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac
     1741 aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat
     1801 ccttcgctgt ggcagttcgg caaggagatg ggcttctctc cccgactgtg tcgccccttc
     1861 agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt acacccgtaa cagtttttac
     1921 atcccactaa tgactcgcct gcgcccgatg gggatcactg tcgatgttga aacagccaac
     1981 cgccacggtc tgcgctggct gcacgatgtc gctgaccaac gaaagcatga aacaatccag
     2041 gcccgtccct gcgatcgctg gctcgaagag cagcagtcca tgctggcact gcctccggag
     2101 aaaaaagagt atgacgtgca tcctggtgaa aatctggtga acttcgacaa acaccccctg
     2161 catcatccac tctccatcta cgactcattc tgcagaggag tggcgtgatg atggaactgc
     2221 aacatcaacg actgatggcg ctcgccgggc agttgcaact ggaaagcctt ataagcgcag
     2281 cgcctgcgct gtcacaacag gcagtagacc aggaatggag ttatatggac ttcctggagc
     2341 atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa acaggcgatg tatacccgaa
     2401 tggcagcctt cccggcggtg aaaacgttcg aagagtatga cttcacattc gccaccggag
     2461 caccgcagaa gcaactccag tcgttacgct cactcagctt catagaacgt aatgaaaata
     2521 tcgtattact ggggccatca ggtgtgggga aaacccatct ggcaatagcg atgggctatg
     2581 aagccgtccg tgcaggtatc aaagttcgct tcacaacagc agcagatctg ttacttcagt
     2641 tatctacgga acaacgtcag ggccgttata aaacgacgct tcagcgtgga gtaatggccc
     2701 cccgcctgct catcattgat gaaataggct atctgccgtt cagtcaggaa gaagcaaaac
     2761 tgttcttcca ggtcattgct aaacgttacg aaaagagcgc aatgatcctg acatccaatc
     2821 tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc agcactgacc tcagcgatgc
     2881 tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa aggagaaagc tatcgactca
     2941 gacagaaacg aaaagccggg gttatagcag aagctaatcc tgagtaaaac ggtggatcaa
     3001 tattgggccg ttggtggaga tataagtgga tcacttttca tccgtcgttg acacattgcc
     3061 ttaatctccg caaagataac ctcgtcattc cggacattct ctgccaggca tatgtcgatg
     3121 taatccataa acgatttcag cttaaccctt ttgtggccgg tctttctggc tggcggttca
     3181 ggggatgttt tctggttatc gtgtaccaca aaactgggat attgccccga tagtcacatt
     3241 tcagattgat cgcggtctga agatgtggca ctgaggtaac gtgctggtgt cactggtatt
     3301 gctgaagtta tggtttttcg gggtcaggtg agcctgttgt gtgtatgctt tttgttcaga
     3361 tcagaggggg gctgtgcgga cagcggcttt tgtctccggg cattcacgcc tgaacgatga
     3421 atgcgatttt cagcaatatt tgtctttgtg acactctctg gttgagaagc taatgcgcgc
     3481 ccctgtttcc acagagaatg aggcttgcca tcagccgaaa catcagtatt ccacgccata
     3541 cagacggcgc ctgaccatgt gctgatgcag gtaaaatgtt tcaactacaa ctgttcgggt
     3601 tgctatgcaa cgaaacagcg attctcacga tctttaactg caacgttttt ccgcctgaaa
     3661 gctgactgcc tcctgctgag gtgggaggct atgattcagt gcgtttatag tctccggggt
     3721 atgcatgtac cggagcatac catttctggt ggataacaag ccggcacatt cagcctgcag
     3781 tgaagatgga ggcacctggt tgtgaagaac gtttctgccc gtggctgctt cgcatcatca
     3841 atggtattca tacccgctca gaaatgaccg ggtgctgaag ccctccgtta tcgcgcagta
     3901 taatgacgta tatgatctgt ggacgagtga gcggcgtgcc tgatgtacgc cgctattact
     3961 gtattttacc acagggctgg ttttctgctg ccagccctgt ggttatcaga catcaactgt
     4021 gagcggcatc aaccgatgta cggtatgcca taagcacgta ctgatccatc agatttgtca
     4081 gtggttcact ctgaccctga cgtgcaatga tccattgctc cagatctttt tctgatttgt
     4141 gtttcaggtt tttcgtccag tcgtcaaggc tgtttccggt cattaagtca agccagcaga
     4201 gccagaccag tttgagacgt aactccgtgc cgccaaactg acggaagagg ctgatcagtt
     4261 tgtttgccag agaatgttgc ttcaggcgct cacattcaga cagcaggatc ccttccagca
     4321 tcggatgatg aatcagcagg tgttcgctgg tgcataaatt caccaccaga tcatcatcca
     4381 gggacgggga gctccgtttg tccggaccat aaatcagccg gtgcagactc tggggaacga
     4441 tggcaatctc cggtttttcg ccattgaatg acacccaccc gggcgtcaga ttatggaaat
     4501 agtagatatt cggatgacac acgccccatg atacggcgtg ccgggtgaag ccggtcagcc
     4561 atttctggta gttttcatgg agcagctcgt aacgggtctg atactgttgt ttcattttca
     4621 tattcctcac gggggataac aggcagcaga gcgccgccgg caggccggag attgcgtctg
     4681 ctttgggcga agggttagtg gttaaaaatt acggacggga ctgtgcaata cgcgcctgca
     4741 gccaggcttc cacttcactt ttcagccagc gggagctgcg ccccattttg atgggggcag
     4801 gaaagccccc aactttgatg agtttgtaaa accacttatc ggttaagccg gtcagttgag
     4861 tgataaacgc catgtcgacc atctggtcat ccatcagcga aactggggta gccatgagaa
     4921 tgttcctccg gatgttgtta aaccagccgg tgtggtcatg aaaacagcgc gccaccttca
     4981 ccggcataac aaccggggag aaacgtaaaa aataatcggg aatctggctg tccggagata
     5041 aacgggggga ttgcgatggc agagccgtag cggaatcccg gggggtgccc ctattaagtt
     5101 gtcaagcatg ttatgacgcc tgcggggtat aaaaagtccc gtcgcgcatc atggcgaaca
     5161 gaacgtcgca gcgtcgtctc gccagggcga taagcgcctg attgtgtcgt tttccctgac
     5221 tcattttgcg ggtgtagtaa gccctggaga gcggatccct gagcgcggcg aaggccgaca
     5281 ggaacaacgc ccgtttgaga gctttattac cccgtcgcga gggatgctca ccgcgtattg
     5341 acgagccgga tcgccgagtt accggcgcaa ggccagcata agcagcgaga tgtgcgacag
     5401 agtcgaaggc gcggcaggcg acctcggtga ggagtctggc tgcggtcctg acaccgactc
     5461 cgggcatact ggtcaggacc gggtaaagag ggtgagcaag aactcgctgt tctaccgcaa
     5521 gcgccacctc gtctctttgc ttacgcagcg tgatgagctg gagtgccaga cgtggcagta
     5581 ctacggcagc ggcattcgtg ccgggaacga cgacggtttg ttcggccagt gcctgagcta
     5641 tgtctgctgc aaggcgttta cccagacgag gcgcaagttt gcagagctgg gctgccagct
     5701 tcttctcacc cagcgaagcg agtttttctg gtgagggata tcgctggaga agatcgagta
     5761 ccgccgggtg atcaagtctc ggaccgagaa cgcgctccag tgccggatgt atctgggtca
     5821 gaaggccgcg gatacggttg ctggcctgcg ttgtctgtgc ggcaagatca tcatcgaagc
     5881 cgcagagcat ggagagttcg gcgatttgct cgtcagccag tttcagcgtg cgtagcgcgt
     5941 gaggcagggt acgggcagct tcggcaatga tggcagcgtc acgagcatca gttttagctt
     6001 caccggcgtg taagtcggct atgcggcgca tggtcagtcc agggaggtat ccgacaagga
     6061 ctccttctga gcgggcaacg gcgacaggta acgcaccgat ggtagctggc tgatcaacaa
     6121 ccagcagtat ctgaccatgt tgtttcaggt cagatattag cgacctgagt ttgttttcgt
     6181 cgttgggtaa tgctttatcg aacaggcgtt tacctgaacg attaatggct acagcgtgat
     6241 gcgtatcttt accgacatca acgccgataa agacctggac ggattcgtaa tcgctggatt
     6301 cggtcattct gtctcccttg tatatgggtt aaccagataa ccacggggag caggtaccgg
     6361 catccacgtt acagacggtc ccggcaaaag tgcctgacct gacccctatt agcggttacc
     6421 agcgccccac cagacccggt gacatcaccc cccggatcat ggacgactgg gggcagtaat
     6481 catgccgggt ctggctggct aacaccccat tataaggggt acgaataaag taacgggggt
     6541 gcgaaaagat gcttccgcac gcacgcgcga taaagagggg atagttttcg tgaggacagg
     6601 gagagaagca ggcgttctga taattgcaga gttgttttcg gaggtggtta cagtgagtca
     6661 gcccgttcct ggcgagcagg agactgatat caccgatggg ataaggttgt ctgattggta
     6721 ttataaatat aagaagtcat cagagagtaa cgtaaaggat ttgccagagt aagctaagag
     6781 agtaaatctc tccggtaaca gagcgctgtg aagcattctg tatcagagtg caaatacatg
     6841 aacgctgatg cgtaaaatca gcagtgacac gccagctccc cctatgccca ttcctgtata
     6901 agacatgaac tgagcgtatt aatgctcctg ccctgagact gagccggtga gtgaggggct
     6961 gagaaatccc tcttgacact gctctaacca gaaatacttc cccggttgtt gcggtgcgtc
     7021 cggatccccg ataacggaac actaaggctg tattgcggga caccaggtaa gtggcagccc
     7081 gtaatacgtc aggagacgcg ggaaaagtga attcccacac gcgttataaa cttacccttg
     7141 cggagctgga agcctttaac tctgccgttg acaaccggct ggcagaactg accgcaaatt
     7201 ttacgagtgc gtgctggctt cagtcttgtc acctgagcgt tcggtaacgg aggcgcgggc
     7261 ggtaatagac tgattttcag gtattaccgg cttacggcgt gtggttgata ttgaggggct
     7321 gtgagtttcg cgtatttgtc agaaacccag acgagttttc ctgtatggga cgaaataagg
     7381 cagcggcaat acctggaaaa atttaatatg gtatgaagga gaacctgtcg gttacgctta
     7441 atatacccgg tacactgtca gctcagggga tacatgaacc gttatctcac cagctacaca
     7501 gtatatacac actgacgctg acagtagtca acaggaccac aataatcagt gccagcagta
     7561 tggtaacgat acggtctgta ttccggtaat agccgaaggg gtcgacagaa ccacaatacg
     7621 ggcaacacac tgtattatga gtaatcgcgt ttccacagtc cctgcacttc tttttgcgta
     7681 tcaccatcac tccctcctca cagccttacc cgtaaccggt tttttacatt aaaaaaacaa
     7741 cgctgaaaat tcaaccgtca gtccggagac gaccgttcgg gttatcacag agtgcctgag
     7801 acagtgccct gccggaggtc acttcaggga ctctgcgcat tttttacgac gtggctattc
     7861 cgtatgaacc atacggagat ttaaccatga cctacaaata caaccccttc tggcagcaac
     7921 gtattcgtga gacagtgcgg cacgcactga atgttcatcc ccgcctgacg gcattgcggg
     7981 ttgacctgcg tttcccggat gtaccggcag caacggacgc agctgtgata tcccgcttca
     8041 tcaatgccct gaaagcccga atcgacgctt accagaaacg taagcatcgg gaaggtaaac
     8101 gcgtgcatcc caccactctg cattacgtct gggcccggga gtttggggag tgcaaaggta
     8161 aaaaacacta tcatctgatg ctgctggtca accgggatac ctggtgtcgt gccggtgatt
     8221 accgcgctcc gggatcactg gccggaatga ttgaacaggc ctggtgcagt gccctggggg
     8281 tggatgccgg atgccatgcc acgctggtgc attttccggc ctggccggcg gtgtggctgg
     8341 cgcgtaatga tgacaccggc tttcagcaag tgctggaacg tgctgactat ctggcgaagg
     8401 agcataccaa agctcactgc accggtgagc gcaactttgg ctgcagtcgt ggctgagcca
     8461 gacagacgag ttatctgact tcagtgtgat gacgtgttca tggccaccac actgattttc
     8521 ccttatgaga ctgaactgaa accgccattc acacctgacc acgctgcccg taccgggacg
     8581 gctgttgcct gcgtgtcgtc tgacggtaag gaacatatac tatgtacgca aaatcctttc
     8641 tcgctcttga tagcaacgga cgtctgacgg gcgcccgtac tgtacaggcc gcaccttatg
     8701 ctcactacac ctgccacttg tgtggcagtg cactcagata ccatccgcaa tacgacactg
     8761 aacttccctg gtttgaacac actgacgaca ggctgacaga gcacggtcaa cagtgccctt
     8821 atgtcaggcc ggagcgcaga gaaatacagt tgattaaacg tctgcagcaa ttcgtaccgg
     8881 atgccttacc cgtggtgcgt aaagccagct ggcactgcag acaatgtcac cacgattatt
     8941 atggggagcg gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc
     9001 aggaggaaat atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt
     9061 tacggcgaaa gaagcagcga agcacaaccg ccaggacgtt ttgtgctgtg tgtcctgtgg
     9121 atgcccgtta acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca
     9181 gatgactgtc gctgagaaaa tcctgctgcg atgcacctgg cttgacccgg cagagaaaga
     9241 ggcccgtcgt ttgcatctgc agggtatgac ggttccggat tatacggtga aggtgagaaa
     9301 gtggttttgt gtgatgtgtg acgaagatta tgaaggagaa aagtgctgcc cgcgttgtgg
     9361 taccggggta tacagcaggg agggggggct gcaggaaggc aactggaagg ataggaactg
     9421 agcggttcag tcgcggaaat cagttgtaga gctgtaatga tatctccggg agtgctgcgt
     9481 tggtaggggc atcccgggat agagaaattt tagcactttg ccctgaatga ttgtagacgt
     9541 cccgggtact cgtaccggtc tgagttatta cagttagata ttcacttttt ctcccgcata
     9601 cttcacatct gtcaacgacg gatgaaaagt ggttcttccg caacctgggt ggaagaaaat
     9661 ggatgaaact ttctggcgaa caaggatcta agctgtttta agttatgggc aacgcaatgc
     9721 actctcttaa gacacttcta cagttacctt gcggatggcg atgcagtcga caaattatta
     9781 gctctgacgg tataaccctc catctccacg gaaaacgcaa aacagcacaa tgtcctgaat
     9841 gctttaagcg tagcgactct gttcatagtt gtcgtcggcg ccggatacag catctaccct
     9901 gctcagggca gacgctatgg cttgtatttt ccgtccgcca ctggtactgc cgtaacccgg
     9961 cttgttaacg taaaattttt gccgagtcgc ttgctccctt cgccggttca caccagcagt
    10021 cttcacaggc gttacaaaat ttacaacgtc aactgggatt aatagctgga ggtgaggctg
    10081 gaaaacgggc tgcaacggca gcgggtctcc gttgcagtgc tgatactctt cttcgcaggg
    10141 ttatcaatac cccggagacg aaacagtcag gcgcgcctca tgtcggtatt gatgagtggg
    10201 cgtggcatcg gggccactgc tgcggtatgt taatagtcaa tcttgatact caccgtcccc
    10261 tcgtcctgct tcccggccgt gatcagcgta cgctggcgac ctggttcaga aaatatccgg
    10321 aaatacaggt tgtctcgcgt gatcgcagtg gagtctatgc gacagcagca cgtgaaggtg
    10381 cacctcaggc cagacaggtg gccgatcgat ggcacctgct aaaaagtatt ggtgatgagc
    10441 ctgaacgaat gatgtacaga catatgcctc tgatacgtct tgttgtcaga gagttatcac
    10501 tgaataaatc acctgagcca gaaatatctg tgcctgtagc atcgctccgt cgtccggaac
    10561 gccttaaaca gcaaacccgc aaaaaacggc atcagcattg gacagaggtt atggccctgc
    10621 ataacaaggg atgtagtttc agggaaatat cccgtattac aggcctgtcg cgagtgacag
    10681 tcagtcgctg ggtgcgttca ggaacattcc ctgaaatgtc aacccggcct ccaaagcgag
    10741 ggcttctgga cccatggagg gagtggttaa aagagcaacg agaaagtggt aattataatg
    10801 ccagccggat atggcgggaa atggtggccc agggggggac aggcagtgaa accatcgtca
    10861 gggatactgt tgccaaatgg cgtaaaggct ggaacccacc ggttactact gccgcaagac
    10921 ttccttcagt gtcccgggta agccgctggt tgatgccctg gagaataatc aggggcgaag
    10981 aaaattatgc atcccgattt attagtctga tgtgtgaaaa agaaccggag ttgaaaatag
    11041 cacagcaact ggtactcgag ttctaccgta ttctgaaaac ctaaaataaa tcacagctaa
    11101 gcagctggtt cactcgagtt cacgaaagcg gctcagcaga acttcggtgc gtagctgcgg
    11161 ggatggaagc tgatgctgcg gctatatgtg aggcaatcag cagtcgctgg agtaatggtg
    11221 ttgtcgaagg tcatgtaaat cgcctgaagg tgctgatacg ccagatgtat ggtcgagccg
    11281 gatttgaact acttaggcgg agggtaatga gtccactggc atgaaaatct tcacctaggt
    11341 tgcggaagag ccacttttgc actgttcgtg acagtatggc ctttatgtac tcactttgtt
    11401 accggtagct gaatcatcac taccgtgctg caaatggtgg tgaacagcat caattgtgga
    11461 atgaatgttg ccgaagcgac caatgcgcct cgtttccacc atcagtggtt gccggactag
    11521 ctgcgtgttg aaaagggttt agtccggata cgctcaagct gctggaagca aaaggtcaga
    11581 aagtggcgct gaatgaggcg atgggcagca cgcaaagcat tatggttggg tcggatggtg
    11641 agttgtacgg cgcatccgac tcgcgcttgg tggatgattt aacggcgggg tattaattga
    11701 catcccccgg cgcagtgtgc cgggggataa tggttttatg cggtcaataa tcgtccggga
    11761 ttatcgatac agagtttttt gaggatcgtt ttatcgacgc cgcgctccgc cagataggcc
    11821 agaaaaacat ctggaacaaa tccccagcca ttaccgccat ttttagccca catttgttta
    11881 aggaagacat cgtggctgag aacaagctga tcggcgtatc ccaactcaat gagatgagcg
    11941 acggcatcgg cagtctcctg cacccctggc gctattccct ctttcgggaa ggtaatgtct
    12001 aggccaatca tgtcgaattc cagccagaca cccttatcaa gcattttccg ctgatacgcc
    12061 acgtcttttc ctgacggatc cgagtgtgcg agagagactt tatttggcga gacgcccatt
    12121 tcacctaaca caatgtcgag tacttcatca ccgcgacgaa gccagcccgg catgtgaata
    12181 ttcatcgcca catgaggatt gttaatctgt gccagcgagg cagcccgcaa gctgttatgc
    12241 tcggcttcgg taaatgtcgg tgagacacct atttcaccga tcattccggc acgaatatcc
    12301 gtatcgccaa tcccttgatt caattcttta tcgatcgttg ccgccagttc atcaaccgtt
    12361 ttatgaattc tctggctttc aaatttttcc aggtagggac cggaggaagc aacaatattc
    12421 agacctgttt ttaatgcgac ttctcgtaat gcctgcgcat ctcgtccgat agactcagac
    12481 cctgtggcat caacaatggt gcgaccaccg agcgagataa agttattaat ttcgaagatc
    12541 acatcttcaa taggtttgcg gtccatatta tcagcgcagc aataaggatc gtgttttaat
    12601 gcccactgga tttctgccgt gactttttta tcaacaagcc gttgtgaaaa aggatagcaa
    12661 ggagcatcca caacagagga aagatcgttg aacagatgtt catgaggaag tgttagcccc
    12721 atatcttcac gggcgacggg gcccgtcaca gtttgtagat aatctttcat gattatacct
    12781 ccattttctt cattgttttc gaggttctgt attccggaat agtggtaaca attgcaccga
    12841 ccagtgcaaa cagtgttccg atgatcgtga ccagataaac ggtattgccc agcgatggaa
    12901 ttaatacgtc aatcaatact gatcccagta actggcccgc cgttgaagca acccccagca
    12961 tcagtaaccc cagacctctg accaaaatgg ccattaaacc gatagacatt agtccaagcg
    13021 ggccgccaag atacatccac cagacatctg gtaattgaac agtgacgtgc cctaatgcga
    13081 cacgaatagc tagcgctgtg cctaaaacac aaaatccaac gatgaaattc caggtgattg
    13141 aaactagcat ggatcccgta gcttcagcta cttttgcatt tccagcgggc tgccatcctg
    13201 caagcaaacc agctaagaac ggaagaatgg caagtaagat gaaagaggta gaatgccact
    13261 gcggtgacac aacaaaaatc gtagcaataa ccgcaaataa tgcaccgatg atgcgataag
    13321 gggtaaagta ttttttctcg tcgacaccga taccaaagcg atcgcaaagt aaaccagaca
    13381 gcaatagcgc ggagatcagt gcggtttgaa acgttgcgac cccgagtgcg ctggctgatg
    13441 cgccttcaga aaataccacc atcgctccac agagaccggc aaaccagttc cagagaggaa
    13501 cttttcggtt tttaatcaga gaaggaattg ccgcaaattg ttgccgggtt tcttttcgcg
    13561 ccataataat aaaaaacatc acgactaaac cactggcaaa ggagataact gcgcatgcat
    13621 taccatcctg taaccagtga cctaattgac cattcaccgc tgattgcata ggggagagca
    13681 tacccgcaag aatcgtggca agcatccata agggagttga gtacttattc tttttcatac
    13741 atcaatcctt ctattatagt aaatggggta actggagtta ccccattatt attatcttgc
    13801 aatatgagtc agtatattta cccacaacgc ggtatagaac ggccaggaca taaactgctg
    13861 tgtaccccag tgcggtgaac agtcactcat aaagcaggct attttcccgt tgtgatagtt
    13921 accaaagacc agtaatggat cgttgtttat tgttaatacc acctcagcat tttctttagc
    13981 gatcgcccgg ttataaccta ggaaaaaagg atattcagag aaccctttaa taactggatg
    14041 ttcaggttgt gatggctgtg caataacacc ctctggtttt tcgacacgat cgtcaccatc
    14101 taacatcgta accgggagca catcagccag aactgtattt ttataattgg cttttgcttc
    14161 aattcccatg aatgagagat agccgccaat catcaataac ccaccaccgt tattcacata
    14221 ttctttaatc agttcgagag cgtttggttt tatgcgaagt tgataaaa
//
LOCUS       DAZFRG010000118        14183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFRG010000118 DAZFRG010000000
VERSION     DAZFRG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.118"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..1214
                     /gene="hmpA"
                     /locus_tag="ACHKUC_002878"
     CDS             24..1214
                     /gene="hmpA"
                     /locus_tag="ACHKUC_002878"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO6619104.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(1259..1597)
                     /gene="glnB"
                     /locus_tag="ACHKUC_002879"
     CDS             complement(1259..1597)
                     /gene="glnB"
                     /locus_tag="ACHKUC_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO6619105.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(1658..2992)
                     /gene="glrR"
                     /locus_tag="ACHKUC_002880"
     CDS             complement(1658..2992)
                     /gene="glrR"
                     /locus_tag="ACHKUC_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO6619106.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGCNRDIDIDVRIISAT
                     HRDLPKAMTRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(2982..3695)
                     /gene="qseG"
                     /locus_tag="ACHKUC_002881"
     CDS             complement(2982..3695)
                     /gene="qseG"
                     /locus_tag="ACHKUC_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO6619107.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(3860..5287)
                     /gene="qseE"
                     /locus_tag="ACHKUC_002882"
     CDS             complement(3860..5287)
                     /gene="qseE"
                     /locus_tag="ACHKUC_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO6619108.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(5863..9750)
                     /gene="purL"
                     /locus_tag="ACHKUC_002883"
                     /gene_synonym="purI"
     CDS             complement(5863..9750)
                     /gene="purL"
                     /locus_tag="ACHKUC_002883"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO6619109.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALINANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVINGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGSEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLVVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DGAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10008..11564
                     /gene="mltF"
                     /locus_tag="ACHKUC_002884"
     CDS             10008..11564
                     /gene="mltF"
                     /locus_tag="ACHKUC_002884"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO6619110.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFVDYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSVELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(11561..12064)
                     /gene="tadA"
                     /locus_tag="ACHKUC_002885"
     CDS             complement(11561..12064)
                     /gene="tadA"
                     /locus_tag="ACHKUC_002885"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO6619111.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(12122..12757)
                     /gene="yfhb"
                     /locus_tag="ACHKUC_002886"
     CDS             complement(12122..12757)
                     /gene="yfhb"
                     /locus_tag="ACHKUC_002886"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO6619112.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            12966..13814
                     /locus_tag="ACHKUC_002887"
     CDS             12966..13814
                     /locus_tag="ACHKUC_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6619113.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            13870..14130
                     /gene="yfhL"
                     /locus_tag="ACHKUC_002888"
     CDS             13870..14130
                     /gene="yfhL"
                     /locus_tag="ACHKUC_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO6619114.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT         3408 a         3863 c         3641 g         3271 t
ORIGIN      
        1 gatgcaaaaa aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca
       61 ccatcccttt actggtggaa acgggaccaa agttaaccgc ccatttctac gaccgtatgt
      121 ttactcataa cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc
      181 aacgtgaagc cctgtttaac gctattgccg cctacgccag caatattgaa aacctgcctg
      241 cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac cagtttccag atcaaaccgg
      301 aacagtacaa catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg
      361 ggcaggaagt gctggacgcg tggggtaaag cctatggcgt cctggctaat gtatttatca
      421 atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc
      481 gcgatttccg cattgtggct aaaacaccac gcagcgcact tatcaccagc ttcgaactgg
      541 agccggtcga cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc
      601 tgaagccgga aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg
      661 atggcaaagg ctatcgtatt gcggtgaaac gcgaagaggg tgggcaggta tccaactggc
      721 tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct
      781 ttatggctgt cgcagatgac acaccagtga cgttaatctc tgccggtgtc ggtcaaacgc
      841 caatgctggc aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt
      901 tccatgcggc agaaaatggc gatgttcacg cctttgccga tgaagttaag gaactgggtc
      961 aatcactgcc acgctttacc gcacacacct ggtatcgtca gccgagcgaa gccgatcgcg
     1021 ctaaaggtca gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca
     1081 gcgatccgac aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttgccgcga
     1141 aacagttagt ggatctgggc gtgaaacagg aaaacattca ttacgaatgc tttggcccgc
     1201 ataaggtgct gtgatttgat gttgccggat ggacacatcc ggcaaccctt gacgcgtttt
     1261 aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat gacccgtgcc acgtcgaaga
     1321 cgaagatttt accgtcaccg attttgccag tttgcgccgt gcgaataatg gtatcgacac
     1381 aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca
     1441 ccatatactc cgcaccgcgg tacagctcgg tatggccttt ctggcggcca aaacctttca
     1501 cctcagtgac cgtcatgccg gtaataccga cttcagccag tgcttcgcgg acatcgtcca
     1561 gcttgaaggg tttaataatc gcatcaatct ttttcatgct attccttgaa aaggtcgcct
     1621 gtcttttgat ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat
     1681 ccagttcgtg tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg
     1741 ccgcgtgggt gacgttgcct ttggtgattt gcagcagctt acgcaaatag ttgagttcaa
     1801 actgattacg cgcctcaaca aaggttggca gcgccgtgtt ttcgccctcc agcgcctgct
     1861 ccaccagcgc atcactaatt accggagatg atgtcagcgc cacgcactgt tcaatcacgt
     1921 tgaccaactg gcgcacgttg cccggccagc tcgcagtcat caggcgtttc attgcatcgg
     1981 tggagaacgc gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg
     2041 ccagtagcgg aatgtcttct gtgcgctccg ccagggccgg aattttcagg ctgacaacgt
     2101 tgaggcggta atagaggtct tcacggaatt ccccgcgcgt catcgctttt ggcaggtcgc
     2161 ggtgggtcgc agaaataatc cgcacgtcga tatcaatatc gcggttacag cccagcgggc
     2221 gcactttacg ctcctgcaac acgcgcagca gtttgacctg caacggtgcg ggcatatcgc
     2281 caatctcatc gagaaatagc gtaccgcctt ccgccgcctg gaataaacct tcgcgattgc
     2341 tgacagcgcc agtaaacgcg ccacgtgcat gaccaaacag ctccgactcc agcaattgct
     2401 cgggtaatgc gccacagtta atagcaataa atggcttgct gttgcgcggg ctggcgttgt
     2461 ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg gccgttaatc aaaacgctaa
     2521 cgtctgattg tgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg
     2581 tgacaatcgc ctcgcgccag cgttcgtcgg tggctggcgc ggattgttcc agcgcatcgt
     2641 caattgcctg atatagcgca tctttatcga caggcttggt gagaaaactg aacacgccct
     2701 gctgtgttgc agcaacggca tcaggaatag aaccatgcgc ggtaagaata attactggca
     2761 ttcccggctg cactttctgg atttcagcaa acagctgcat accgtccatt tcatccatcc
     2821 gcaggtcgct gatgactaaa tctacttttt cgcgattcag tacccgcaat ccttcagcac
     2881 cactttccgc cgtgaccaca ctgtagcctt cgctggtcag gcgcaggcca agcagtttca
     2941 gcaatcccgg atcgtcatcg accagtaata aatgcgcagg tttatggctc atcaggagtg
     3001 acctcatcgg tggatggcgc gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt
     3061 ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg
     3121 gtagtgagtt ccagttgctg ttgtagaacg tgatgttgct ggcgcaaagt atccagctcg
     3181 ctgtcgctgg actgctggag tttgctgtag cgttggcgct cttccgccag ttgcagttgc
     3241 agcgcctgac cgtcgcgcca aagttgatac agtgggcgaa cctgtgccgg gatctcggta
     3301 cttagcgcat caatgcgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg
     3361 gcgagcaaaa tcccttgttt aaaggtattt tgccagttgc cgtcgtcata ttggcgggct
     3421 tgctgacgcg actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa
     3481 agcggattgg tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa
     3541 agatagtcag ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc
     3601 ttattgtgac tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc
     3661 cgtcggggca ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc
     3721 agtgggcgta attgttgcag gcagtttgga catggacagc gcggagaaac cagagcgtac
     3781 atgtcgtacg tgaagatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg
     3841 atgggatggg ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac
     3901 aaacgtcttg cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat
     3961 ccctggcaat gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc
     4021 cctgaaaaaa gggttcgaag atcatggcgc gctcctcttg cggaatgggc gtgcctgtat
     4081 tgatgacatc aatataaacc cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg
     4141 attcagcccc gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca
     4201 ttggctccgc caggcaagct gttgctttga gatcaacgtc ggtatgcatc attttagccc
     4261 gtgcgggcag gctatgagca gaaaccactg tctccaccag cggtgctaac tcaacattct
     4321 ccagttccac cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagct
     4381 tttgcaaatt gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa
     4441 gcggcccgac aacctgatca gccagtaatt cagtgccctc gcgcatactc gccagtggcg
     4501 tttttaattc atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca
     4561 ggcgctcact taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga
     4621 acgagacgct attgcccaga gaacgccctt cccccagccg gttgatcatg cgctcgatat
     4681 ttttcaccgg accgataatc atcctcgtga aaagcagtac catcaccaga ctcaccagaa
     4741 atagcaccag cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt
     4801 gctgcccacg agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg
     4861 caaaggcttc cagacgcgcg gcggcagcag catcgggacc gctgttgtta cactgaagtt
     4921 gagccagatt attcaaatcc tgacgtaatg cctggtagag tttatcgtcc ggcagcacgc
     4981 ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa
     5041 gcgtagggtc gtccagcacg cagtactgac gatagctgcg ctccatctcc agtgccgcgt
     5101 tggtcatcgc ttcactgcgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct
     5161 gatcgctaag tgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggcagca
     5221 gaatcagcaa aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc
     5281 gtttcaaggc gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca
     5341 gatacaacaa agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga
     5401 cgtttcgccc gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt
     5461 gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa
     5521 tgagccactg cttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt
     5581 actgattata cgctgctttg aggggtttat ggcttctccg ctatttgaat gtaaatcagc
     5641 cactgtgtcg ctaaaaagag acaacttaag ataaacttat tagataatat taaaatcaag
     5701 gagttaagtg tcaccaaaaa gcgacacgac aaaccaccca ttgtcgtgat ttaaagacac
     5761 aaaaaagctc ccgaagttgg gagctcataa tagcggttgg tgcttaatgc cggatgcggc
     5821 gtgaatgtct catccgggct gcaataccaa tgggttgacg acttacccca actgcttacg
     5881 tgcattgcgg aaaatgcgca tccatgggcc atcctcgccc cagttttccg gatgccagga
     5941 gttgctgacg gtacggaaaa ctcgttccgg gtgcggcatc ataatggtaa ctcgaccact
     6001 ttcagtcgtg accgccgtaa taccgttcgg tgaaccgttc gggttagccg ggtaggtttc
     6061 agtgactttg ccgaagttat cgacatagcg cagtgccacc agccctttgc tttccagtgc
     6121 cgccagatgc gccccatcac gtacttccac gcgcccttca ccatgagaga cagcaatcgg
     6181 catctgcgag cccaccatcc cctgcaacag cagcgacggg ctttgggtta cttctaccag
     6241 actgaaacgc gcttcaaagc gatcggaggt gttacgcaca aaacgtggcc acaactcgct
     6301 acctgggatc agttcacgca gattagacat catctggcaa ccgttacata cccccagcgc
     6361 cagcgtttgc ggacgatgga agaaggttgc aaactcatcg cgtacacggt cattgaacag
     6421 gatagacttc gcccaacctt caccagcgcc cagcacatca ccgtaggaga aaccaccgca
     6481 cgcgaccagg gcgtggaaac cctccaggcc cgtgcgtcca gccagcagat cgctcatatg
     6541 cacgtcgata gcatcgaagc ccgcacggtg gaaagctgcc gccatttcaa catgggagtt
     6601 cacaccctgc tcacgcagta cggcaacttt cgggcgcgcg ccagtggcaa taaacggtgc
     6661 tgccacatct tcgttgatat caaacgacag tttcacattc aggcccggat cggcgtcgtt
     6721 tgatttcgcc tggtgttcct gatcggcaca ctccgggttg tcacgcaggc gctgcatctg
     6781 ccaggtcgtt tctgcccacc agacacgcaa cgtggtgcgg ctttcgctaa atacagtctg
     6841 cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc tgcccaacat aatggacgca
     6901 atcagcaagc ccatgctgtg ccagtaccgc ttcaacggct tcacggtcag cggctcgcac
     6961 ctgaatcacc gcacccagtt cttcgctaaa caatgcagcc agacgatcgt cacccagagt
     7021 ggcgatatcc gcgtcaatgc cgcaatgacc agcaaaggcc atttccgcca gcgttaccac
     7081 cagcccgcca tcagagcggt cgtgatacgc gagcagctta cgctgtgcaa ccagcgcctg
     7141 aatcgcgtca tagaagcctt tcagttgcgc gacatcgcgt acatctgccg gtttgtcgcc
     7201 aagctgacga taaacctgcg ccagcgccgt tgcgcccagc gcgttattgc ctttgcctaa
     7261 atcaatcagc agcagtgcgt tatcttcggt agaaagctgc ggcgtgatgg tgtgacgtac
     7321 atcttccacg cgggcaaatg cagaaatcac cagcgaaagc ggcgaggtca tttcgcgctc
     7381 ttcgctacct tcctgccagc gggttttcat cgacatagag tctttaccca ccggtatcgt
     7441 caggcccagc gccggacaaa gttcttcacc cacagcttta acggcttcat acaggccagc
     7501 atcttcacca gggtggcctg ccgccgccat ccagttggcg gaaagtttaa tgcgtttgat
     7561 atcgccaatt tgtgttgcgg cgatgttggt tagcgcttca ccgactgcca gacgggcaga
     7621 agcggcgaaa tccagcagcg caactggcgc acgttcgcca attgccatcg cttcgccgta
     7681 gtagctgtcg aggctggcag tagtgaccgc acagttagcg accggcacct gccacggccc
     7741 caccatctga tcgcgcgcca ccatgccggt tacgctgcgg tcaccgatgg tcaccaggaa
     7801 agttttctcc gccacggtcg gcagatgcag cacgcgtttc accgcgtcag cgatggtgat
     7861 cccttcacgg gccagcgcgt cgcctttcgc tttcagcgtt tgtacatcgc gggtcatctt
     7921 cggcgttttg ccaagcagta cgtccagcgg cagatcgatg ggttgattat caaaatgacg
     7981 atcgtgcaga gaaagatgta attcttcggt ggcctcgcca atcaccgcgt agggtgctcg
     8041 ttcacgctta cacagttcgt caaacagcgg taactgatcg gcagcaaccg ccagcacgta
     8101 gcgttcctgg gattcgttac accagatttc cagcgggctc atgcccggtt cgtcgctcag
     8161 gatatcgcgc agttcaaatt taccgccgcg cccgccgtcg ctcaccagtt ccggcatggc
     8221 gttagaaaga ccgcctgcgc caacgtcgtg gataaacagg attgggttgg catcaccaag
     8281 ctgccagcaa cggtcgatca cttcctggca gcgacgctcc atctccgggt tgtcgcgctg
     8341 tacggaagca aagtcgaggt cggcatcaga ctgaccagat gccatagaag acgccgcgcc
     8401 gccgccaaga ccgatgttca ttgccgggcc gccgagaacg accagcttcg caccaacgtt
     8461 gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg atcccacccg ccagcatgat
     8521 cggtttgtga taaccgcgca gctcttcgcc attgtggctg ttcacttttt cttcataagt
     8581 acggaagtag ccgttcagtg ccgggcgacc aaattcgttg ttaaacgccg cgccgcccag
     8641 cgggccttcg gtcatgatgt ccagcgcggt gacaatgcgc tcaggcttac cgaaatcttc
     8701 ttcccacggc tgttcaaagc ccggaatacg caagttggat acggagaaac caaccagacc
     8761 ggctttcggc tttgcgccac gcccggtggc accttcatcg cggatttcac cgccggaacc
     8821 ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg ttgtgggttt caactttcat
     8881 cagaatatgc gccggttcct gatggaaatc gtagcggccc gtttcgtggt cagcaaagta
     8941 gcgacccact tcagaacctt ccatgacggc ggcgttatct ttataagcag agagaacgtg
     9001 gtctggcgtg gtttcgaagg tgtttttgat catcttaaac agcgatttcg gctgctgttc
     9061 accattgata acccagtcgg cgttaaaaat tttgtggcgg cagtgctcgg agttcgcctg
     9121 cgcgaacata tacagctcga tgtcgttcgg gttacgaccc agctttgtga acgcatcctg
     9181 cagatagtca atttcatctt ccgccagtgc caggccaaga cgcaggttag cgttgattag
     9241 cgcctgacgg ccctgcccca gcaaatctac gctggtgacc ggagtcggtt gatggtgtgc
     9301 aaacaactgc tctgcatcat ctaaagcaaa aaagaccgtt tccatcatgc ggtcatgcag
     9361 ttcagcggtc acctgctgcc attgttcatt ggtcagcgta ccggcttcta tatagtaagc
     9421 cacgccgcgc tcaaggcggt ttacctgttg tagaccgcaa ttatgggcga tatcggtcgc
     9481 tttcgaagac cagggagaga tggtgccagg acgcggggtc accagcagga gtttgccttg
     9541 cggggcgtgg ctggcgagtg ccgggccata tttcagcagg cgttcaagtt gtgcgtgctc
     9601 atcatcgttt aacggcgcat tgaggtcagc aaaatggaca tactcggcgt aaatattgtg
     9661 aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg ttgattcgga atgccgacaa
     9721 tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg tcttctaagc tttcggtgta
     9781 cccaaggggg ggaaacgggc gtcattataa agaatctgat gcgctgacga aaccgtttgc
     9841 gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc gccaatggct gtaataagtt
     9901 gccatctggc gcaggtttac gcaaaatgcc gctcatttat gagtaaacct ttcactatta
     9961 ttacgttttt tcaagctggg acgcgcacga cacagagaat taactaattg aaaaaattaa
    10021 agattaatta tctgttcatc ggcattctgg cactgctgct cgcggtcgct ctctggccat
    10081 ccattccctg gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg cggggagagt
    10141 tgcgtgtgag caccattcat acccccctga cttataacga aatcaacggg aaaccttttg
    10201 gcctggatta cgaactggcg aaacagtttg tcgattacct cggcgtaaaa ctgaaagtga
    10261 ccgtgcggca gaatatcagc cagctgtttg acgacctcga taatggtaac gccgacctgc
    10321 tggcggcagg cctggtctat aacagtgagc gggtaaaaaa ttatcagcct ggccctacct
    10381 attattccgt gtcacaacaa ctggtttata aagtcggtca gtatcgccca cgtacgctgg
    10441 gcaacctgac ggcggagcaa ctcaccgttg caccgggtca cgtggtggtt aacgatctcc
    10501 agaccctgaa agacacaaaa ttcccggaat taagctggaa ggtagacgac aaaaaaggct
    10561 ctgtggaatt aatggaagat gtcatcgaag gaaaactcga ttacaccatt gctgattctg
    10621 tcgccatcag cctgtttcag cgcgttcacc cggaactcgc cgtagcgctc gatatcaccg
    10681 atgaacaacc ggtgacctgg tttagcccgt tagatggcga taataccctt tccgccgccc
    10741 tgctcgactt cttcaacgaa atgaatgaag acggtacact ggcacgcatt gaagagaaat
    10801 acctggggca tggcgatgat tttgattacg tcgatacgcg cacattttta cgcgccgtcg
    10861 atgcggtact gccgcagtta aagcccctgt ttgagaaata cgccgaagaa attgactggc
    10921 gtttgctggc cgctattgct tatcaggaat cgcactggga tgctcaggcc acttcaccga
    10981 cgggtgtgcg cggcatgatg atgttaacca aaaacaccgc gcaaagcctc ggaattacgg
    11041 atcgtaccga tgccgaacag agcatcagcg gcggcgtgcg ttatttgcag gatatgatga
    11101 gtaaagtgcc ggaaagtgtg ccggagaacg aacggatctg gtttgccctc gccgcgtaca
    11161 atatgggcta tgcgcatatg ttggatgccc gcgccctgac ggcaaaaacc aaaggtaatc
    11221 ctgacagttg ggctgacgta aaacagcgtc tgcctttact tagccagaaa ccctattaca
    11281 gcaagctgac ttacggctac gctcgtgggc atgaagccta cgcttatgtc gaaaatattc
    11341 gtaaatatca gattagcctg gtgggttatc tgcaggagaa agagaagcag gctacagaag
    11401 cggcgatgca actggcgcag gattatccgg cggtatcgcc tacggagttg ggcaaagaga
    11461 aatttccttt tctctcgttt ctttcccagt cgtcatcaaa ctatttgacc cactctccct
    11521 ctctgctgtt ttccagaaaa gggagtgaag agaaacaaaa ttaatctgtc gaggattgcg
    11581 cttttttctg cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg
    11641 cacactcatc cgccagtatt ccttccgtaa tttccacccg gtgattcata cccggatgat
    11701 gcagcacatc cattaaagat cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca
    11761 cgcgaccaat gcgactgtgg atcatcgctc cggcacacat tacacagggt tcaagcgtga
    11821 catacaacgt ggcgtcgatc agtcgataat tttgcatcac cagaccgccc tgccgcagag
    11881 ccatgatttc tgcatgtgcg gtgggatcat gtcgaccaat cggacggttc cagccttcgc
    11941 cgattacccg attgttatgc actaataccg cgccgaccgg tacttcccgc tcatcccagg
    12001 cacgcttcgc cagcgtcatt gcatgacgca tccagtattc gtggctaaat tcgacttcag
    12061 acaaaaagaa aactccggtt ataaaagcgc ggcgcattat acacggacgc tatgctttac
    12121 tctattccag ttgctggagt tcaccgcgcg gggttacccg ccagcgatgc tggcaaaagt
    12181 aaagcagcgg gttgtcctgg ttactgtcgc tatagccact atacagccgc agcggcgtgc
    12241 cgattttgcg ctccagttgc gcgacctttt catgtcccag acaacgcatc gtcaataccc
    12301 aaccaccata gccacgctga atctgactgg cgataagatt aacccgcggc agccagggcg
    12361 tatcgaaata aaccgcttca accagcggct gcggagagcc ggtaatcaac catatatcag
    12421 catcggaact taacaggtag gtggttaatc gctcctgaac cagcggaaag gcggtaacat
    12481 tgtcacgaaa ccagcgtacg aaatcggcct gcaacgcctg caaacgcgct tcattgtgac
    12541 caaaagtgca tccccacaga agcagactca tcggccagcg tgccgcacga ccttttatca
    12601 ataacgcaat ggctatgatc ggcaacaacg gcagaacaag taacgcattc agcggttggc
    12661 gacgcagtaa ataacgtaaa aaactaccga acatatcctg ctgatgcaat gttccatcta
    12721 agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc atactcctct ggactgaaac
    12781 atcctgatta attttcgcta ctacatagcc taacagatag atcatcactt ttccggcaac
    12841 aatcgaccgc atcatggcta actggaattt ttaattcatg gcaattagcg gcaatggaat
    12901 ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg ccactatttc atcgcgagga
    12961 aagatatgaa cggcttactt cgtatccgtc agcgttacca ggggcttgcc cagagtgata
    13021 aaaaactggc ggattatctg ctgctacaac ctgatacggc gcgccattta agctctcagc
    13081 aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg
    13141 gctataaagg ttttccggca cttaagttag cgttgagtga agcgctggca agccagccgg
    13201 aatctccctc cgtgcccatt cataaccaaa tccgcggtga tgatccgtta cggctggtcg
    13261 gcgaaaaact gattaaagaa aatactgccg ctatgtacgc aacgttaaac gttaatagtg
    13321 aagagaaact gcatgaatgc gtggcaatgt tgcgctctgc gcggcggata attctgaccg
    13381 gtattggcgc ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg aagattggct
    13441 tcaatgctgc cgcagtgcgc gatatgcatg cgctactcgc tacagtacag gcgtcgtccc
    13501 ctgacgatct gttattagcc atttcctaca ccggtgtacg acgcgagtta aacctggcgg
    13561 cagatgagat gctgcgagtg ggcggaaaag tgctggcgat taccggcttt actcccaatg
    13621 ccctgcaaca gcgtgcttct cattgcctgt ataccattgc cgaagaacaa gcgacaaaca
    13681 gtgcttcaat ctctgcttgt cacgcgcagg gaatgttaac ggatttgctg ttcattgcgc
    13741 tgattcagca ggatctggaa ctggcaccag aacgtattcg tcatagcgaa gcgctggtga
    13801 aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag
    13861 agtttcctta tggcgctgtt aatcactaaa aaatgcatca attgtgatat gtgtgaaccc
    13921 gaatgcccga atgaggcgat ttcaatgggc gatcatatct acgagattaa cagcgataag
    13981 tgtaccgaat gcgtagggca ctacgagaca cccacctgcc agaaggtgtg cccgatcccc
    14041 aatactattg tgaaagatcc gcaacatgtc gagacggaag aacagttgtg ggataaattt
    14101 gtgctgatgc accacgcgga taaaatttaa tattttgctc tggaagtaga gtgttaatta
    14161 tcttatagag aaaccttaac gct
//
LOCUS       DAZFRG010000119        14034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFRG010000119 DAZFRG010000000
VERSION     DAZFRG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.119"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..515
                     /gene="hns"
                     /locus_tag="ACHKUC_002889"
     CDS             102..515
                     /gene="hns"
                     /locus_tag="ACHKUC_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO6619115.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(659..1567)
                     /gene="galU"
                     /locus_tag="ACHKUC_002890"
     CDS             complement(659..1567)
                     /gene="galU"
                     /locus_tag="ACHKUC_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO6619116.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEDEMGIKK"
     gene            complement(1769..2782)
                     /gene="rssB"
                     /locus_tag="ACHKUC_002891"
     CDS             complement(1769..2782)
                     /gene="rssB"
                     /locus_tag="ACHKUC_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO6619117.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRVLFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2874..3779)
                     /gene="rssA"
                     /locus_tag="ACHKUC_002892"
     CDS             complement(2874..3779)
                     /gene="rssA"
                     /locus_tag="ACHKUC_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO6619118.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRTVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3892..4350
                     /gene="ychJ"
                     /locus_tag="ACHKUC_002893"
     CDS             3892..4350
                     /gene="ychJ"
                     /locus_tag="ACHKUC_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO6619119.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVAQFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            4400..5242
                     /gene="purU"
                     /locus_tag="ACHKUC_002894"
     CDS             4400..5242
                     /gene="purU"
                     /locus_tag="ACHKUC_002894"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO6619120.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            5402..5486
                     /locus_tag="ACHKUC_002895"
     tRNA            5402..5486
                     /locus_tag="ACHKUC_002895"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5436..5438,aa:Tyr,seq:gta)
     gene            5533..5664
                     /locus_tag="ACHKUC_002896"
     ncRNA           5533..5664
                     /ncRNA_class="other"
                     /locus_tag="ACHKUC_002896"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            5918..7117
                     /locus_tag="ACHKUC_002897"
     CDS             5918..7117
                     /locus_tag="ACHKUC_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO6619121.1"
                     /translation="MPARQVCQNFFRGALAPFHKYRQNALLDATIALINGASLTLTSI
                     GRYLPGNAQVKNKIKRVDRLLGNESLHHDIPLIFRNIISMLTSKLSLCVIAVDWSGYP
                     SQEYHVLRASLICDGHSIPLLSWIVPSEKQQNAKIQQAFLNTLSEAVNPKARVIIVTD
                     AGFQNAWFRHIKSLGWDFIGRIRGNKQLHLARKGECWFRRQELQAGNKPEYLGPGTLS
                     RAEYARCDGHFYLHKKEPKGRRNKRSRCGIARPSQIKDARSAAKEPWLIFSSTDDFKP
                     REIMKLYSRRMQIEQNSRDEKSERFGFGLRASYSCSAGRMLVLSLLATLSTIVLWLIG
                     YHAENQGLHLRYQANSIKTRRVISYLRLAENVLRHSPLILKRTVLSTILNHLTRTYQN
                     MVLVYYR"
     gene            complement(7170..7847)
                     /gene="narI"
                     /locus_tag="ACHKUC_002898"
     CDS             complement(7170..7847)
                     /gene="narI"
                     /locus_tag="ACHKUC_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6619122.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7847..8557)
                     /gene="narJ"
                     /locus_tag="ACHKUC_002899"
     CDS             complement(7847..8557)
                     /gene="narJ"
                     /locus_tag="ACHKUC_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6619123.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8554..10092)
                     /gene="narH"
                     /locus_tag="ACHKUC_002900"
     CDS             complement(8554..10092)
                     /gene="narH"
                     /locus_tag="ACHKUC_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6619124.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENLEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10089..13832)
                     /gene="narG"
                     /locus_tag="ACHKUC_002901"
     CDS             complement(10089..13832)
                     /gene="narG"
                     /locus_tag="ACHKUC_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO6619125.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIARKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3454 a         3719 c         3426 g         3435 t
ORIGIN      
        1 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
       61 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
      121 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
      181 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
      241 ctgctgctga agttgaagag cgcactcgta aactgcagca atatcgcgaa atgctgatcg
      301 ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca
      361 aagctaagcg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
      421 aaacctggac tggccagggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
      481 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acatgctaaa
      541 aatcccgcca tcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
      601 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg aaaacaccga tacggatgtt
      661 acttcttaat gcccatctcg tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
      721 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
      781 tcttcccttt catatgatag gcttcaaccg tttctttttc gatcagcata tcaattgcgt
      841 cggtgagctg aatttcatca ccagctccag gaggggtttt tgccagcaac ggccaaatat
      901 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgcttttg
      961 gtttttcaac cacgccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     1021 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     1081 ccgtttcatc aaagcggcgg atcatctctg ccaggttatc ctgtgataaa tcggattcat
     1141 attcatccag aataacgtca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
     1201 gagcacacaa taccgcgtgt cccaggcctt tcgccagccc ctgacgaact tgcataatag
     1261 tcacgtgtgg cggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     1321 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     1381 agtgagtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     1441 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     1501 acatcctggt tcctaatccc gctacgggga taacggcttt tttgactttc gtattaatgg
     1561 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     1621 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gttagcataa
     1681 ttaagacaat tacttatacg aatagcgacg atagtaccac cagccgacat tagcaggtaa
     1741 tgcaaattta gcccgtgtta tcgtttgctc attctgcaga caacatcaag cgcagccgac
     1801 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     1861 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctga tgttcgccgg
     1921 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     1981 aatagccaac taatagcgga aactgccccg gcagattggc ctgacggagt aaatggttta
     2041 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     2101 gtaataatcc gttaaacaat acgcgtaata acaaggcagc aagtacgcca ttatgtccag
     2161 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     2221 gcaccaggcc gggtttgtcc gcggcaacta attgacgata attaacccgg cattgggaaa
     2281 tcacctgctg aactggcggt tgtagttcct gtaataattt cgccgctgca gcagggttat
     2341 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgg gaattaaaca
     2401 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaaccggtt
     2461 tcagcaaaac atcttcaaca cccagacgta acgctttggc aatatctgcc atattttcag
     2521 tggcagatat caccagaact ggtgtctggt cacctctatt gcgtatatgt tccagcagtt
     2581 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     2641 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     2701 aaaaccacga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     2761 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcacg
     2821 ctattgccac ctacgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     2881 ttggtgcgta ccaacggcaa aagttcgtcc attttctttt ccactgccag ctgtccggcc
     2941 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     3001 atcagaatat cgggcggatc acctgccatg cggttccttt taagtcggtt ctccagcacc
     3061 tggatagaag tggtcataat ctctgttgcc gttggcgctg tcaccgtccg acgtgtcgtt
     3121 atgctgccta acctttcttt cagacgcgca tgccacggca gagaatcacc attctcgcta
     3181 ttttcttcac tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc atcgtgctgc
     3241 aggtcgaccg ctatcacaat atcagccccc aatgcacgcg tgagggaaat aggaattggg
     3301 ttaactacag cgccatcaac cagccagtag ccgttatgcg caacgggtgc cattagtcca
     3361 ggaatactgc atgatgcacg aatggcaaga tggagatcgc cttcagtaaa ccataattca
     3421 cgcccagtac ttaaattggt ggcaacagcc gcgaagcgac gggaacaatt ttcgatctct
     3481 gtttccggca ttatttcgcg atattgattg aagacacgct cgccgcgcag taacccaccg
     3541 cgctgccagg agagatccat caggcgtaaa acatcccaat aactgaaaga ggtcacccaa
     3601 tcttccagcg cagataatcg atcgcatgca taggcagcgc ccaccagcga accaattgaa
     3661 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat tacgccaata
     3721 tgcgaccaac ctcttgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
     3781 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
     3841 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
     3901 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
     3961 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
     4021 caagacgcag attatttaat aaagacctgg catccatctt gtggagcagc ggcgttacgt
     4081 gccgaattga ttgccggatt tgcgcatact gaatggttgg gcttgaccgt atttgaacat
     4141 tgctggcagg acggcggcaa tatcggattt gtcagttttg ttgctcaatt taccgagggt
     4201 ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
     4261 tatattgacg gtacacgtcc gcagtttggc cgcaacgatc cctgcccttg tggttcaggt
     4321 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
     4381 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
     4441 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
     4501 cgtgcagaac aatgaatttg tagatcatcg taccgggcgc ttttttatgc gcacggaact
     4561 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
     4621 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtaattctgg tcactaaaga
     4681 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggcctgg atgtcgaaat
     4741 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcggt ttgatattcc
     4801 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
     4861 tgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
     4921 gccggaattt gtggcacgct tcccgaataa gatcatcaat attcaccatt cattcctgcc
     4981 agcgtttatc ggcgcacgtc cttatcacca ggcctatgag cgtggagtga agattattgg
     5041 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
     5101 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
     5161 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggcg cagcgcgtct ttgtttacgg
     5221 taatcggacg attattcttt aatcgccagc aaaaataact ggttagcttt aatccgttac
     5281 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
     5341 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
     5401 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
     5461 aggttcgaat ccttccccca ccaccatcac tttcaaaagc ccctgaattc tcaaacgaat
     5521 ccgcaattaa atattctgcc catgcgggga aggatgagag gcttcgacca aggttcgact
     5581 cgagtgccag cgagagagcg ttgccgcagg caacgacccg tagggcgaag cgcgcagcgc
     5641 tgagtaatcc ttcccccacc accatttctt cctgaaatac tcaaaacctg ttactccgag
     5701 ctaattctgc ccaatggcac cgcgtttacc ggacctaaaa tatcctcccc tgtaggtcgg
     5761 ataagcgaag cgcatccgac aactcccccc gcaccgtgcc cataaaaaaa acccgccgga
     5821 gcgctgagga atccccagta atgggcgggt aaaaaaagac aggcatagag ttttgtgatc
     5881 tactgacttg tgcaaccaat tcaatgagat ctcctctatg cctgctcgcc aagtatgtca
     5941 gaattttttc cgcggtgctt tagcaccatt tcacaaatac cgacaaaatg ctttgttgga
     6001 tgccactatc gcactaatca atggcgcatc tctgacgctg accagcatcg gacgttattt
     6061 acccggtaat gctcaggtca aaaataaaat aaaacgtgtt gatcgtctgc tcggtaatga
     6121 gtctcttcat cacgacatcc ccctgatttt cagaaacatt atttccatgc tgacgagcaa
     6181 gctgtcactg tgtgtcatcg ccgttgactg gagtggatac ccctctcagg agtatcacgt
     6241 actccgcgcc agcctgattt gcgatggaca ttcgattcca ttgctgagtt ggatagttcc
     6301 ttcggaaaaa cagcaaaatg caaagataca gcaagccttt cttaatactc tttccgaagc
     6361 ggtaaaccca aaagcaaggg taatcatcgt cacggatgca gggttccaga atgcctggtt
     6421 tcggcacata aaatcactcg gctgggattt tattggccgg atacgaggca ataaacaact
     6481 gcatctggcg agaaaagggg aatgctggtt caggcgtcag gaattacagg ccggtaacaa
     6541 acctgaatat ctggggccgg ggacgctttc ccgggcggaa tacgcccgtt gcgatggtca
     6601 tttttatttg cacaaaaaag agccgaaagg ccggagaaat aaacgttcgc gttgcggtat
     6661 agccagacca tcacagataa aagatgccag gtcggcggca aaagaaccat ggttaatatt
     6721 cagcagcaca gatgacttta aaccgcgaga aatcatgaag ttatacagcc gccgaatgca
     6781 gattgagcaa aactccaggg atgaaaaaag tgagcgtttt gggttcggtc tgcgagccag
     6841 ttacagctgt tcggcaggaa gaatgctggt actgagcctg ctggcaacac tgagcacaat
     6901 agtgctgtgg cttattggct atcacgctga aaatcaaggg ttacatctta gatatcaggc
     6961 caacagcatt aaaacgcggc gggtaatctc gtatctgagg ttagcggaga atgtcttacg
     7021 acactccccg ctaattttaa aacgaacggt gctaagcacc attcttaatc acctcaccag
     7081 aacctaccag aatatggtgt tggtttatta tcgctgattt atggggatcc ctcagcgccg
     7141 aagcagggtc tatgtgaact aaaattcgct tagtgacgag cgcgcaccag ctgatactta
     7201 cgcgtcagat actccaccgg gacgctccag atgtgtacca gacgcgagaa cgggaacagc
     7261 aggaataacg tcatccccaa caccaggtgc agacggaaga taaacgccac gccatcaaga
     7321 tgttgagaag cgccaccgtg gaaggtcact acagactgcg cccagccaac cagtttcatc
     7381 atctcgctac cgtccatatg ctgggcggag aacggaatgg tcagcaggcc cagcgcgcac
     7441 tggataacga gcagcgacag gatcaagata tccgctccgg tagtcgttgc acgcacgcgt
     7501 gggctgaaca gacgacgttt caacaacagc acgccgccaa tcagacacag tacgccgctg
     7561 gcaccaccag caaacatcgc cattttctgt ttcacttcaa tcggcagcca ggcttcatac
     7621 atccagtgcg gcgtcagcat accgaagaag tgaccgacaa aaatccccag aataccgata
     7681 tggaacaggt tcgacgccag attcatccct ttgcgatcca gcatctggct ggacgccgca
     7741 cgccatgtgt actgcccgta gtcataacgc agccagctac caatcaggaa gaccgccccg
     7801 gctatgtacg ggtagatatc aaagaagaac atattcagga attgcattag tgctgtcctc
     7861 cggtggtgat attcagatat tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag
     7921 tgattgctga atcaccgcag cctttgtcag caaagaattt aacctgctct tcttcccaga
     7981 ctgcatccag cgcctgcggc gtatcgtcgc gcgcttcatc ggcaattttt tccgccactt
     8041 tgtcgctgtc gattgcggta ttcgccagtt tcagcagcag atcaaacagc acggcataac
     8101 ggctttcacg ctgttgcaga cgcgcgctca gcaatgccag aatcggcgca atatctttca
     8161 aaccctccac ggcttcgctt tgcggcagct gcgccaggta ctccagatac agcggcagat
     8221 ggtcgggcaa ttcgcggctg tttaactgca agccgtgctg ctcgtactgc gccagcagat
     8281 ccaccatcgc ctggccgcgg tcacgggatt caccgtgaac atgttcaaat agcaacagtg
     8341 acgtggcgcg gccacggtcg aacagttcgc tgtactgcgc ctgggcatcg agcggatcca
     8401 tcgtcgttaa atcgcgcagg aaaatgccca gcgcatgggc atcctctttt ggcagatttt
     8461 tcgacgcggc aatcgcctca aacatctctt gttgatgctg ccataaggca gcatccggat
     8521 attcaaggag acgcgataca atcacgagtt cgatcatggg tgcggctccg ttttgctggt
     8581 tacatcgatg gcatcgatac ggcggctgtt gaacagattg aatttggtat ctgaaccgtg
     8641 gcagccatca ccaaaggtaa agccgcagcc atttttctcc gggaaggctt cccgcgccag
     8701 ttcacgatga ctgctcggca ccacaaagcg atcttcgtag ttggcaatag ccagataacg
     8761 atacatctcc tgtgcctggg cttcggtcag accgacctct tccagcgcac gggtatcgac
     8821 tttaccgtca acggtttcag cacgtttgta atgacgcatc gccagcatac gtttcagcgc
     8881 gcgcagtacc ggtttggtat caccggcggt cagcagattc gccagatact gtaccggaat
     8941 acgcaggctt tcgacgtctg gcagaatgcc gttgctgccc agttcacccg cgtctgccgc
     9001 agactgaatc ggcgacagag gcggcacgta ccagaccatc ggcagtgtgc gatattccgg
     9061 atgcagcggc agcgccagtt tccattccat cgccatttta taaactggcg actgctgcgc
     9121 agcctcaata acgctcagcg gaataccgtc tttaatcgcc tgctcgatga ctttcggatc
     9181 gttcggatcg aggaacacat ccagctgacg ctggtagaga tctttctcgt tctcggtact
     9241 tgccgcacgt tcaatcgcgt cggcatcgta cagcagcacg ccaagataac ggatacgacc
     9301 gacacaggtt tctgaacata ttgtcggctg gccggcttca atacgcggat agcagaagat
     9361 gcacttctca gacttaccgc tcttccagtt gaagtagatt tttttgtacg ggcatccggt
     9421 gatgcacata cgccagccac ggcatttatc ctggtcgatc aggacgatgc catcttcttc
     9481 acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat gccgggttca gacagtgttc
     9541 gcacaggcgc ggcaaataca tcatgaaggt gttttcgaac tggctataca tcgccttctg
     9601 gatgttgtcg aagttcttgt ctttcgccag cttgtcaaac tcaccgccca ggtcatcttc
     9661 ccagttcggc cctttttcga ttttcgccat ccgttcgccg gtaatcagcg aacgcggacg
     9721 ggcaatcggc tgtgatttgc tgccttccgg cgcggtatgc aggttctgat aatcaaaatc
     9781 gaacggctcg taataatcgt cgatccccgg cagatgcggg ttagcgaaga ttttacccag
     9841 cagcatggca cggttaccca tgcgcggctg cagtttgccg ttgattttac ggatccagcc
     9901 gcctttgtat ttttccaggt tttcccagtc agtcgggaag ccctggcccg gcttggtttc
     9961 cacgttgttg aaccacgcgt attccacgcc ttcacggctg gtccagacgt ttttacaggt
    10021 cactgaacag gtgtggcagc cgatgcactt atcgagattc agcaccatgc cgacttgtga
    10081 acgaattttc attttacgct ctcctgtacc tggtcattgc cttcgccatc taaccagtca
    10141 atgttcttca tcttacgcac tacaacaaac tcatcgcggt tagaacctac ggtgccgtag
    10201 tagttaaagc catatgccag atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg
    10261 atacgggtga ccgagttatg gataccacca cgctgttggg taatttccga accaggcagg
    10321 ttaacgatac gttcctgcgc gtggtacatc atggtcatcc ctgccggaac acgctggctg
    10381 acaaccgcac gggcagtcag tgcaccgttg ctgttgaaga cttcaatcca gtcgttatcg
    10441 gcgataccca gctctttggc atcggcttca ctcagccaga ccaccggacc accgcgacct
    10501 aaagtcagca tcagcaggtt gtcgctgtag gtggagtgga taccccactt ctggtgtggc
    10561 gtcaggaagt tgagcgcttt ttccgggttg ccattggatt tctggcccat cacttctttc
    10621 accgaacggg tgtcgatcgg cggacgataa accagcaggc tttcaccgaa atcacgcatc
    10681 cattggtgat cctgatacag ctgctgacgg ccagagagcg tacgccacgg gatcagctcg
    10741 tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat cttcaaggcc agaccatgtc
    10801 gggctggaga tgattttgcg cggctgtgcc tgaatatcgc ggaagcgaat cttctcgtct
    10861 tctttattca gcgccagatg cgtatggtca cgaccggtaa attcgctcag ggcagcccag
    10921 gcttttacag ctacctgacc attggtttcc ggtgccagtg tcaggatcat ctctgccgca
    10981 tcaattgcgg tgttcagcat cggctggcct ttcgccggac cttccgcttt ggtgtagttg
    11041 agcttacgca gcagatccat ctcgctctgg gtgttccagg caatcccttt accgccgtta
    11101 ccgattttct ccatcagcgg gccgatagag gtaaagcgtt cgtaagttgc cggataatcg
    11161 cgctctacga ccataatgtg cggtgcagtt ttacctggga tcaggtcgca ttcgcctttt
    11221 ttccagtctt tcacatccag cggctgcgcc agttcagcgg cagagtcgtg ctggataggc
    11281 agcgtgacga tgtcagtttc tttacccaga tgaccaacgc acacttcgga gaatttcctg
    11341 gcgatggctt tgtagatttc ccagtcgctt ttcgcttccc aggccggatc gaccgccgca
    11401 gacagcgggt gaataaacgg atgcatatcc gaagtattca tgtcgtcttt ttcgtaccaa
    11461 gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc tcgacagacg gaagtccagc
    11521 gtcaccacca gatccagctt gccttccaga ccgttgtcct gccagtccac ttcttccggc
    11581 ttcacgccgc cctgctgccc caggtctttg ccctggatac cgtgctccgt ccccagcagg
    11641 tacttgagca taaactcatg acctttaccg gaagaaccga gcaggttaga acgccagatg
    11701 aacagattac gcgggtggtt tttaccgttt tccggctgtt ccgcagcgaa acggatagaa
    11761 ccctctttca gggatttcac cgtatagtcc accggattca tcccggcttt ttctgcttcg
    11821 cgagcgattg tcagcgggtt agtgcctaac tgcggcgcag acggcagcca gcccatgcgt
    11881 tctgcacgga cgttaaagtc gatcaagtgt ccggtatagc gagatttatc cgccatcggc
    11941 gacagcagtt cttccgcagt cacggtttca taacgccact ggctggagtg gttatagaaa
    12001 taagaggtac tgttcatgtg acgcgccgga cgctgccagt cgagggcaaa cgccagcggc
    12061 tgccagccgg tttgcggacg cagtttttcc tgacctacat agtgcgccca gccaccgccg
    12121 ctctgaccga cacagccgca gaaaatcagc atattgatca gaccacgata gttcatgtcc
    12181 aggtgatacc agtggttcag cccagcgccg acgataatca tcgaacgacc gtgcgtttta
    12241 tcagcgttat cggcaaattc acgggcgatg cgaataatct ggctgcgaga aacgccggta
    12301 atctgctctg cccaggcagg ggtgtaagct ttcacatcgt catagctggt tgcacagtta
    12361 acgtcgttca ggccacgttc cagaccgtag tttgccagcg tcagatcata aacggtggtc
    12421 accagggcgg tgctgccatc agccagttgc aggcgtttca ccggcagctt atgcagcagc
    12481 acgttttcca gttccacttt gttgaagtgt tccgtgccat cgccaccaaa gtacgggaag
    12541 cccacctcag cgatctcatc ctggctaccc agcaggctca gttgcagctc ggtttcttcg
    12601 ccagttttgc cgtcgcgctg ctcaagattc catttgccct tctcgcccca gcggaagcca
    12661 atagaaccgt tcggcgcaac catttcacca ttggtattaa aggcgacagt tttccattcc
    12721 ggattgtttt cctggcccag tgcatccacc agatcagcag cgcgcagcat acgacccgca
    12781 gcgtagtaac cgtcgcgctc ttccagcatc accagcatcg gcatgtcggt gtagcggcgc
    12841 acatagtcgg tgaaatactg gcttgggttg tcgaggtgga attcacgcag cattacgtga
    12901 cccattgcca gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac
    12961 agtttggcga tttcagcgta gtctggtgtg acggcaacag ttttggttcc tttgtaacgc
    13021 acttcagtaa agaagtgagc atccggggta cgcgtctgcg gcacgtttga accccaggcg
    13081 atgatgtagc tggagttgta ccagtcagca gattccggta cgtcagtttg ctcaccccag
    13141 gtttgcggag acgcaggagg caggtcgcag taccagtcgt agaagcttaa gcaagtaccg
    13201 ccaatcagcg agagatagcg cgcacccgat gcgtaagaaa ccatcgacat tgccggaatt
    13261 ggcgagaagc cagcgacacg gtccggaccg taatttttga tggtgtaaac gttagatgcg
    13321 gcgatcagtt cgttcacttc ctgccaggaa gaacgaacaa aaccaccgcg tccacgcgcc
    13381 tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag aagcccatgc ctcaaccgga
    13441 tcgctatgca gcgccttcgc ttcacgccac attttcatca ggcgtttgcg catcatcggg
    13501 tatttcaggc ggttagcgct gtaaagatac caggagtagc tggcaccgcg cgggcagccg
    13561 cgaggttcat ggtttggcag gtccgggcga gttcgcggat agtcagtctg ctgggtttcc
    13621 caggtgacca gaccgttttt gacgtagatt ttccagctgc aggagccggt gcagtttacc
    13681 ccgtgggtag agcggacgat tttgtcatgc tgccaacgct ggcgatatcc atcctcccag
    13741 tcacggttgg tattgagaag ctggccatgc ccatcggcaa aggtttcacc cttctgtttg
    13801 aagtagcgaa accggtccag gaatttactc atcggttttc tcctgtggga gcctgtcggc
    13861 cctctgataa atcgacattg ctaatgttta agggcaaggt aacgctctga aacagcgtgg
    13921 gaattgataa cgatcaagag tgatggggat gaaaaataaa gtaaattcct ggaatacccc
    13981 tttaggataa ttaacattta tagcataaca gactgtaaat taagacgttt taca
//
LOCUS       DAZFRG010000120        13871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFRG010000120 DAZFRG010000000
VERSION     DAZFRG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.120"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..962
                     /gene="ydjJ"
                     /locus_tag="ACHKUC_002902"
     CDS             <1..962
                     /gene="ydjJ"
                     /locus_tag="ACHKUC_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO6619126.1"
                     /translation="VLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVV
                     AVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLC
                     HPESFTYKLPDNMDTMEGALVEPAAVGMHAAMLANVKPGKKIIILGAGCIGLMTLQAC
                     KCLGATEIAVVDVLEKRLTMAEQLGATVVINGAKEDTIARCQQFTEDMGADIVFETAG
                     SAVTVKQAPYLVMRGGKIMIVGTVPGDSAINFLKINREVTIQTVFRYANRYPVTIEAI
                     SSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKISD"
     gene            983..1819
                     /gene="ydjI"
                     /locus_tag="ACHKUC_002903"
     CDS             983..1819
                     /gene="ydjI"
                     /locus_tag="ACHKUC_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO6619127.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            1824..2771
                     /gene="ydjH"
                     /locus_tag="ACHKUC_002904"
     CDS             1824..2771
                     /gene="ydjH"
                     /locus_tag="ACHKUC_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6619128.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            2781..3761
                     /gene="ydjG"
                     /locus_tag="ACHKUC_002905"
     CDS             2781..3761
                     /gene="ydjG"
                     /locus_tag="ACHKUC_002905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO6619129.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            3898..4656
                     /gene="ydjF"
                     /locus_tag="ACHKUC_002906"
     CDS             3898..4656
                     /gene="ydjF"
                     /locus_tag="ACHKUC_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6619130.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            4774..6132
                     /gene="ydjE"
                     /locus_tag="ACHKUC_002907"
     CDS             4774..6132
                     /gene="ydjE"
                     /locus_tag="ACHKUC_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6619131.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMHWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(6225..6866)
                     /gene="pncA"
                     /locus_tag="ACHKUC_002908"
     CDS             complement(6225..6866)
                     /gene="pncA"
                     /locus_tag="ACHKUC_002908"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO6619132.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(6877..7893)
                     /gene="ansA"
                     /locus_tag="ACHKUC_002909"
     CDS             complement(6877..7893)
                     /gene="ansA"
                     /locus_tag="ACHKUC_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO6619133.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGALIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASYRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8060..9916)
                     /gene="sppA"
                     /locus_tag="ACHKUC_002910"
     CDS             complement(8060..9916)
                     /gene="sppA"
                     /locus_tag="ACHKUC_002910"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO6619134.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SIRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10077..10628
                     /gene="ydjA"
                     /locus_tag="ACHKUC_002911"
     CDS             10077..10628
                     /gene="ydjA"
                     /locus_tag="ACHKUC_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO6619135.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10745..11788
                     /gene="selD"
                     /locus_tag="ACHKUC_002912"
     CDS             10745..11788
                     /gene="selD"
                     /locus_tag="ACHKUC_002912"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO6619136.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11793..13739
                     /gene="topB"
                     /locus_tag="ACHKUC_002913"
     CDS             11793..13739
                     /gene="topB"
                     /locus_tag="ACHKUC_002913"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6619137.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKHFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGIVASGGGA
                     VKKAPNKRVKKAAKPKTDNTDSAN"
BASE COUNT         3540 a         3195 c         3636 g         3500 t
ORIGIN      
        1 aagttttgat taaagtggaa tatgtcggta tttgtggttc agatgtacat ggttttgaat
       61 caggcccgtt tattccgcct aaagacccaa atcaagaaat tggcctgggt catgaatgcg
      121 ccgggacggt tgtggctgtg ggaagccgcg tgcgcaaatt taaaccgggg gatcgggtaa
      181 atatcgaacc tggcgttcct tgcggtcact gtcgttactg tctggaaggc aaatataaca
      241 tttgcccgga cgttgatttt atggcgacac aacccaacta ccgcggcgca ttaacgcact
      301 atctgtgtca tccggagagc tttacttaca aactgccaga caatatggac acgatggaag
      361 gggcgctggt ggagcctgcc gcagtcggga tgcatgccgc gatgctggca aatgttaaac
      421 ctggtaagaa gataattatt ctgggagcgg gttgtattgg tttgatgacg ttgcaagcgt
      481 gcaaatgcct gggagcaacg gaaattgccg tcgttgatgt gctggaaaaa cgtctgacaa
      541 tggcggaaca gctcggcgcg acagtggtta ttaacggcgc aaaagaagac actattgcac
      601 gctgtcagca atttaccgaa gacatgggcg cagatattgt ttttgaaaca gcgggttctg
      661 cggtcaccgt taaacaggca ccttatctgg tcatgcgcgg cggtaaaatt atgattgttg
      721 gtactgtacc cggcgattcg gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc
      781 agacggtatt ccgctatgcc aatcgttatc cggtcacgat tgaagctatt tcttcagggc
      841 gattcgatgt gaaatcgatg gtgacgcata tttacgatta tcgggatgta caacaggcat
      901 ttgaagagtc agttaacaac aaacgcgaca ttattaaagg cgttattaaa attagcgatt
      961 aattaatacg ggagaacaga acatgctcgc agatatcaga tattgggaaa acgatgccac
     1021 aaataaacat tatgcaattg cccattttaa cgtatggaat gcagaaatgt tgatgggcgt
     1081 tatcgacgca gccgaagaag cgaaatcacc ggttattatt tcttttggca caggttttgt
     1141 cggcaacacc tcatttgaag atttctctca catgatggta tcaatggcac aaaaagcaac
     1201 ggtgccggta ataactcatt gggatcatgg tcggagtatg gagattattc ataacgcctg
     1261 gactcatggt atgaattcat taatgcgtga tgcttctgca tttgatttcg aagaaaatat
     1321 tcgtttaacc aaagaggctg ttgatttctt ccatccgctg ggtattccgg tagaggcgga
     1381 attagggcat gttggtaatg aaaccgttta tgaggaagcg ctggcgggct atcactatac
     1441 cgatcctgac caggcggctg aatttgttga acgtacgggc tgtgactcat tggctgtcgc
     1501 catcggcaac cagcatgggg tttatacgtc agagccacaa ttgaactttg aggtcgtcaa
     1561 acgcgtacgc gatgctgttt ctgttccgct ggttttgcat ggcgcatcag ggatcagtga
     1621 tgccgacatt aaaactgcaa tttcgttggg tattgcgaaa atcaacattc atacggagct
     1681 ctgtcaggcc gcaatggtag cagtgaaaga aaaccaggat cagcctttcc tgcatttaga
     1741 acgtgaagtg cgtaaagctg taaaagaacg ggcattggat aaaattaaac tgttcggttc
     1801 tgatggcaaa gcggaataat aatatggata atctcgacgt tatttgtata ggtgccgcta
     1861 ttgttgatat tccattgcaa ccggtcagta aaaatatctt tgatgtggat tcttatcctc
     1921 ttgaaagaat cgcaatgacc accgggggtg atgcaataaa tgaagcaaca attatttctc
     1981 gtctgggcca tcgcacagca ttaatgagtc gtattggtaa agatgccgca gggcaattta
     2041 ttctcgacca ttgccgcaaa gagaatattg atattcaaag cctgaagcag gacgtcagca
     2101 tagatacctc tattaacgtt ggactggtga cggaggatgg tgagcggacc tttgtcacaa
     2161 accgtaacgg cagtctgtgg aaattgaata ttgacgatgt tgattttgcg cggttttctc
     2221 aggcgaaatt attatcactg gccagtattt tcaacagtcc tctattggat ggtagagcgc
     2281 taacagaaat ttttacgcaa gctaaagccc ggcagatgat tatctgtgcc gatatgatca
     2341 aaccgcggtt gaatgaaacg ctgaatgata tttgcgaagc attgagttat gtcgattatc
     2401 tgtttcctaa ttttgccgag gcaaaattac tcaccgggaa agagacactg gatgaaattg
     2461 ctgactgctt tcttgcgtgc ggcgtaaaaa cggtggtgat taaaacgggt aaagacggct
     2521 gctttatcaa gcgtggtgac atgacgatga aggtgccggc ggtcgcagga ataaccgcta
     2581 tcgacaccat tggtgcgggc gataactttg cttcaggttt tattgcagca ctgttagaag
     2641 gtaaaaatct gcgtgaatgc gcacgctttg ccaatgcaac ggcggctatc tcggttctaa
     2701 gcatcggcgc caccactggt gtaaaaaaca gaaagctggt cgaacaattg ctggaagaat
     2761 acgaaggata atgaaggcaa atgaaaaaga tacctttagg cacaacggat ataacgcttt
     2821 cgcgaatggg gttggggaca tgggccattg gcggcggtcc tgcatggaat ggcgatctcg
     2881 atcgacaaat atgcattgat acgattcttg aagcccatcg ttgcggcatt aacctgattg
     2941 atactgcgcc aggatataac tttggcaata gtgaagttat cgttggtcag gcgttaaaaa
     3001 aactgccccg tgaacaggtt gtagtagaaa ccaaatgcgg cattgtctgg gaacgaaaag
     3061 ggagtttatt caacaaagtt ggcgatcggc agttgtataa aaacctttct ccggaatcta
     3121 tccgcgaaga ggtagaagcc agcttgcaac gtctgggtat tgattacatc gatatctaca
     3181 tgacgcactg gcagtcggtg ccgccatttt ttacgccgat agctgaaact gtcgcagtgc
     3241 ttaatgcgtt aaaggccgaa gggaaaattc gcgctatagg cgctgctaac gtcgatgctg
     3301 accatatccg cgagtatctg caacatggtg aactggatat tattcaggcg aaatacagta
     3361 tcctcgaccg ggcaatggaa aacgaactgc tgccgctatg tcgtgataat ggcattgtgg
     3421 tacaggtcta ctctccgcta gagcagggat tgttgaccgg gaccatcacg cgtgattacg
     3481 ttccgggcgg cgctcgggca aataaagtct ggttccagcg tgaaaacatg ctgaaagtga
     3541 ttgatatgct tgaacagtgg cagccactct gtgctcgtta tcagtgcaca attcccactc
     3601 tggcactggc gtggatatta aaacagagtg atttaatctc cattcttagt ggggctactg
     3661 cgccggaaca ggtacgtgaa aatgtcgcgg cactgaatat caacttatcg gatgcagacg
     3721 caacattgat gagggaaatg gcagaggccc tggagcgtta aatactcaac acgattcacc
     3781 tctgtttctg tgaaaactgt gatgaaaatc tgtgattcaa acaggttatt ttgaaagtaa
     3841 acatcggtta tgcgataatt gcgctaatgt gatgtaaaaa gatacagggg tgtaatcgtg
     3901 gcggcaaaag acaggattca ggcaattaag caaatggttg ccaacgataa aaaggtgaca
     3961 gtctcaaatt tgagtgggat ttttcaggta accgaagaaa ccattcgccg cgatcttgag
     4021 aagttggaag atgaaggctt tttgaccaga acctatggtg gtgctgtttt aaatacagcg
     4081 atgttgacgg agaatatcca tttttataag cgcgcttcat cgttttatga agagaagcag
     4141 cttattgcac gcaaggcact cccctttatc gacaataaaa ccaccatggc agccgattcc
     4201 agtagtaccg taatggaatt gctcaaattg ttacaggacc gtagtgacct gacgttgcta
     4261 accaactcgg cggaagcgat tcatgttctg gctcagtcag aaattaaagt cgtttcaacg
     4321 ggtggggaac taaacaaaaa tacactttca ctgcaaggaa gaattactaa agagatcatc
     4381 agccgctacc atgtcgatat catggtaatg agttgtaaag gtcttgatat taacagcggc
     4441 gcgctggact ctaatgaagc ggaagctgaa atcaaaaaga caatgatccg tcaggcgaca
     4501 gaagttgcgt tattggttga tcactctaag tttgatcgca aagcttttgt ccagttagct
     4561 gactttagtc atattaatta cataataact gataaatcac cgggtgcaga gtggatagca
     4621 ttttgcaaag acaataatat ccagctggtg tggtaattta agcacacatt gtcaatatta
     4681 tttgttcctg cgcctggaaa taaaatgatg ataaagatgc ggcaagtgaa tttgccgcaa
     4741 ttgctacctt ttttagcaaa tccgaggcac ccaatggaac aatatgatca aattggcgca
     4801 agactggacc gcttgccttt ggcccggttt cattatcgta tatttggtat aataagcttt
     4861 agtctgttat taacggggtt tttgagttac tccggtaatg tcgtcttagc aaagctggta
     4921 agcaatggat ggtcaaataa tttcctcaat gccgccttta cctcggcatt aatgtttggt
     4981 tatttcatcg gctctcttac cggtgggttt attggtgact actttggacg gcgcagggcg
     5041 tttcgcataa atcttctcat cgtcggtatt gcggcaacag gggccgcttt tgtccctgat
     5101 atgcactggc tcatcttctt tcgcttcctg atgggaacag gaatgggggc gctgattatg
     5161 gttggctatg cctcatttac ggagtttatc cccgcgacgg tgcgtggaaa atggtccgcg
     5221 cggctctcat ttgttggtaa ctggtcgccc atgctgtctg cggcgatagg cgtggtggtt
     5281 atcgcttttt ttagttggcg gataatgttt ctgctgggcg gtattggcat actgttagcc
     5341 tggtttctct caggtaaata ctttattgag tcgccacgat ggctggcagg gaaagggcaa
     5401 atcgcaggtg cagaaagcca acttcgtgaa gtagagcagc aaattgaaag agagaagagt
     5461 attcgtttac ccccgcttac tttgaaccag agcaacagca aggttaaggt aatcaagggt
     5521 accttctggc tcctgtttaa aggggaaatg ttacgacgta cattagtcgc gattactgtt
     5581 ttaattgcaa tgaacatttc gctttatacc atcaccgtat ggataccgac catatttgtt
     5641 aactccggca ttgatgttga taaatcaata ttaatgaccg ctgttattat gattggcgct
     5701 ccggtaggga tatttattgc ggcattaatt attgatcatt ttcctcgtcg attatttggc
     5761 tctgccttac ttattattat tgctgtgtta ggctatatct attcaattca gactacagag
     5821 tgggcgattt taatctatgg cctggtgatg atcttctttt tatacatgta tgtgtgcttc
     5881 gcgtcggcgg tttatatccc ggaactttgg ccaacgcatt tacgcctgcg tggttcgggt
     5941 ttcgttaatg ccgtcggacg gatcgtcgca gtcttcacgc cctatggcgt tgcggcatta
     6001 ttaacacatt atgggtcgat cacggtgttt atggtgcttg gtgtcatgtt agtgctctgt
     6061 gcgctggttc tctccatttt tggcatcgaa acgcggaagg tgtcgctgga agagatttct
     6121 gaggtgaatt aaatctttcg ttgatggcgt caattaccag atgtatcact gatgtgtttt
     6181 atctggccta caatgccagt acagaattgt aggccagcgt aaaattactc ctgcgtttct
     6241 tcccagtctg ccagcgtata tagcgttgcc ccagctgctg acatctccat aaacgcgtgc
     6301 gcactgtcct gtggctggat atttacgcca cgacaaccat cggtaatcac gttcacctta
     6361 tagcctaact gtaacgcgtc cagcacggta aacttaacgc aatagtcagt agccagaccc
     6421 ataacgatca attcattgat tacatgagcg cgcaaccagt catcgagcgc agttttctgc
     6481 cgacggccgt tatcaaaaaa ggcactgtaa ctgtcaacta aaggattttc gcctttatgg
     6541 aacaccgctg cgatcgcttt ttgcttcagt aacggatgta attgtgcgcc ttcactgttc
     6601 tgcacacagt gatcgggcca gaaggtttgt ggcaaaccgt cgagttggcc cggcgtataa
     6661 ggctctacac cgtgctgact ggcaaaactg ccgtgattcg ccgggtgcca gtcctgactg
     6721 gcgataaccg cttcaccgcg cgactggcac cagtcaatca ggcggttagc gacatccacc
     6781 gtactgtcac cttccggcac ggcgagcgcg ccaccagcac agaaatcatt ttgtaaatcg
     6841 accagtaaca gggcgcgagg gggcattgca gtctccttaa tcatccggcg tcagttcgcc
     6901 acgcaggttt tggctcatgg ccttgcgaat ggtttcagta tcaagttcct ggctaagcag
     6961 gtaatgcagt ttggttagcg tggcttctac agtcatatct gcaccgccaa taacgccggc
     7021 atgggcgagg gcgttaccag tggcataacc acccatgttc actttaccgg acatacattg
     7081 tgtcaggttg accaccacaa taccgcgata gctggcttct tgtaattcct gcaggaaggc
     7141 tttgttttgt ggcgcattac ccacgccata tgagcgcaga atcaatgctt tcacaggttg
     7201 gcgcagaaaa ttgcgcacga cgtcagcaga aatccccgga taaatcgtta ctacgccaat
     7261 tggttgcggc gtgattggat gaacgatcaa tgccccttca ccgtgcgggg cgggtggcgt
     7321 attcaaacgg cgaatatgga tacctgcttc cagtaacgga ggaaggtttg gagaggcaaa
     7381 cgcatcaaaa ccatcggcat gggctttggt ggttctgttg ccacgataca gtcgattatt
     7441 aaaaaagaga gttacttcgt tgataggata attcgccgca acgtacaacg cattcagcag
     7501 attaatttgt ccgtcagagc gtaactcagc cagcgggatt tgtgaccctg tcacaatgac
     7561 cggtttaccg agattctcga gcatgaacga cagtgcagag gcggtatacg ccatcgtgtc
     7621 tgtaccgtgc agaatgacaa aaccatcata atcgtcatag tgcgctttaa tatcttcagc
     7681 aatatgctgc cagtcttctg gcgtcatatc agaagaatcc atcaatggcg tatattcatg
     7741 aatggtgaaa tctggcatct ccgggcgatg gaattccggc atcagcgcca gttggcgctg
     7801 tagatgacct gacaccggaa tataaccctg ctcggaacgc tgcatcccga tggtcccgcc
     7861 cgtgtaggcg acgtaaattg acttcttttg catgatattg atatgaatga tatcgaaaga
     7921 gcaaaagtat atacgtgagg cagggggagc gcacctgata ctatgaggat aaggaaatat
     7981 gtggcgagac agcgggaaga ggattcgtcg acgtaaaaag taatgtggcc gacgatctgt
     8041 cggccactta gtacaagact taacgcacgt tggcgcaggt caggcaaaac gcataacggt
     8101 tttgtgggtc gttaaacgcg gccagcttgt cgctttcact tttaacggta gaggctaccg
     8161 aggccagcgg tgcaggtaac atggcctgga acgcatctgg caacattgcc cggatagaac
     8221 cagacatgtt gtccatcact ttgtcgaaga aggtcggttc atcaacgtag tattccagat
     8281 gccactgttt cacttttgcc agctctgctg ctttggcgac cgcatcatcg aaatccccga
     8341 gactatcgac cagcccgtta gcttttgcat cctgaccggt ccagacgtga ccctgggcga
     8401 ttttatcaat ctgctccggc gttgaatgac gcgcatcagc aaccagcgtg ataaagcgtt
     8461 tatagccatt ctcaatgctt aactgcatca tctgctgcgc ttccggcggc agtgccctgg
     8521 tgatagaaac atctgccagc ggtgaagttg agacaccatc agtatgaaca ccaatcgaat
     8581 ccagactatt ttctacggtg gtgatcacgc cgaagatacc gatagaaccg gtcagggtgc
     8641 tggggttagc cacaatgtaa ttagctggtg tggaaatcca gtaaccacca gatgccgcca
     8701 tgccacccat cgatacaacc acaggcttac ccgctgcccg ggctgctgcc agttcagcgc
     8761 gaatcacttc agacgcggta acgctgccgc cgggactatt aacacgcaga acaatcgctt
     8821 tcactttcgg gtcaaggcga gcgtcgcgga tttgtgccgc agtggtatca ccgccaacat
     8881 tcccctgagt ttcctcgcca tccataattg cgccattagc gaagacgaca ccgatactgt
     8941 caccagtatc tgccggcgtt ttcaatgcgt aatcgtaata actgatggcg cgataatttt
     9001 tatcagtttt actccagccg aactctttgg tcagagtttt ttcgatttcg gcactcgatg
     9061 ccagtgcatc gaccagcttg ttttccagcg catatttcgc ggtatcgcca ccggttttgg
     9121 ttaaaccctc aagcaaccct tgcgcgccag ggaatacctg ctgagcaggg atctggcggt
     9181 tagcggcaac agtattcaga tagttttgcc acagctcacc aatccagcgg ctgtcagctt
     9241 cgcgggctgc cggcgacata tcatcacgaa taaacggttc aacggcagat ttatacgtac
     9301 ccacgcggaa cacatgggtg gaaactttca gcttatccag taacgatttg tagtacagac
     9361 cgttggtggc aaagccgtgc agatcaacca cgccttgcgg agacagccaa attttattgg
     9421 cgaaactggc gagataatat tgcccctggc tgtagttctc gccaacggca taaaccggtt
     9481 tcccgctatc acgaaactct ttcagagctt tgccgatgta ctgcatagac ggctggtcgc
     9541 cgcctgcgaa gtttttcaga tccatcacaa taccggtgat attgcggtcg tccttcgcct
     9601 ggcgaatggt gttgacgata tcaaacagtg agttttcctg cagacgatcg gaactggcac
     9661 caagcagctg gcggcttaat ttactaaacc gctgagaact gtcgggttta tcgacgatca
     9721 caccagaaat gtccagcagc agtgcgccac gactggccgt ttctttcgaa tcaccaccac
     9781 tgacctgcat ccagatcccc acaccaacca gcacgagaaa aataaagaac aggttaagta
     9841 ccatttcacg gacgaaattc agcagacgcc acgtccattt aaaaaatccg gcaataaatc
     9901 gccaaagggt tcgcatgtat tctcccaact taaggtcaca cctgtttcag gcgcaataga
     9961 tgtggttatc gtaatgaccc aaccggcact tgtcagcagg aattgcggac tgggctgtaa
    10021 caaaatccac tctcgtgtta attttgtgaa taaatatcat gacaggagtt aatcaaatgg
    10081 atgcactcga actattgatc aatcgccgta gcgcctcccg cctggctgaa cccgcgccaa
    10141 cgggtgaaca actgcaaaac atcctgcgtg cgggtatgcg tgcgccggac cataagtcca
    10201 tgcaaccgtg gcatttcttt gtgattgaag gggaagggcg cgagcgtttc agcgctgtac
    10261 tggagcaggg ggcgattgct gccggtagtg ataacaaagc aatcgacaaa gcgcgtaatg
    10321 cgccgttccg cgcaccgctc atcataacgg tggtggcgaa atgcgaagaa aatcataaag
    10381 tcccgcgctg ggaacaggaa atgtctgcgg gatgcgcggt catggcgatg caaatggcag
    10441 cagttgccca ggggtttggc ggcatctggc gcagtggtgc attaactgaa agtccggtag
    10501 tccgtgaagc attcggttgc cgtgagcagg ataaaattgt cggttttctc tacctcggta
    10561 cgccgcagtt gaaagcatct acgtcgataa acgtcccgga cccgacgccg tttgtgactt
    10621 atttctgata attgtcgcga aactgtctgg attgtgagca aaggcgcagg aattcagaca
    10681 ctctcactta tcacttcatg gaatgagggt tatcataccc ggattgcatt gacaggagat
    10741 gtccatgagc gagaactcga ttcgtttgac ccaatacagc cacggcgctg gttgcggctg
    10801 taaaatttcc ccaaaagtgt tggaaaccat cctgcacagt gagcaggcga agtttgttga
    10861 tccgaatttg cttgtgggta atgaaacccg cgacgatgcg gcggtatacg atctgggcaa
    10921 tggcaccagc gttatcagta ccaccgactt ctttatgccg atcgttgata atcctttcga
    10981 ttttggccgc attgcggcga ctaacgccat cagcgatatc tttgctatgg gcggcaaacc
    11041 gattatggcg attgcgatcc tcggctggcc gattaacaaa ctttccccgg aaattgcccg
    11101 cgaagtgacc gaaggtggac gctatgcatg ccgtcaggcg gggattgcgc tggctggcgg
    11161 tcactccatc gatgcgccgg agccgatttt tggtctggcg gtaacgggga tcgtaccgac
    11221 cgagcgggtg aagaaaaaca gtaccgcaca agccggatgc aaactgttcc tgacgaaacc
    11281 gctggggatt ggcgttctta ccacggctga gaaaaaatca ctgctgaaac cagaacatca
    11341 gggactggcg acggaagtga tgtgccggat gaacatcgca ggcgcgtcct ttgccaacat
    11401 cgaaggcgta aaagcgatga ccgacgttac gggttttggt ttactggggc atttaagcga
    11461 aatgtgtcag ggcgcaggtg tgcaggcgcg cgtcgactat gacgcgatcc cgaaattgcc
    11521 gggggttgaa gagtacatta agttgggcgc agtgcctggc ggcaccgaac gtaactttgc
    11581 cagctacggt catctgatgg gtgaaatgcc gcgtgaagtg cgcgatctgc tgtgcgatcc
    11641 gcaaacttct ggcggtttgc tgctggcggt catgccggaa gcagaaaatg aggtcaaagc
    11701 tacagccgcc gagtttggca ttgaactgac ggcgattggc gaactggtgc cagcgcgcgg
    11761 cggtcgtgcc atggttgaga tccgttaatt caatgcggtt gtttattgcc gaaaaaccga
    11821 gtctggcgcg cgccattgct gatgtcctgc ctaaaccgca ccggaaaggc gatggcttta
    11881 tcgagtgcgg taatggtcag gtggtgacct ggtgtatcgg tcacctgctt gagcaggcgc
    11941 agccagacgc ctacgacagc cgctatgcgc gctggaatct tgcggatttg ccaattgtcc
    12001 cggaaaagtg gcaattacag ccccgaccct ccgtgactaa acaacttaat gtcatcaaac
    12061 acttcctgca tgaagccagc gaaatcgttc acgccggaga cccggatcgc gaagggcaac
    12121 tgctggtgga tgaagtgctg gactacctgc aactagcacc ggaaaagcgc cagcaggtac
    12181 agcgttgttt gataaacgac ctgaacccgc aggcggttga gcgggcgatc gatcgtcttc
    12241 gctccaatag cgagtttgta ccgctgtgcg tttctgcact ggcgcgggcg cgggctgact
    12301 ggctgtacgg cattaatatg acccgtgcgt ataccattct cggccgcaat gccggttatc
    12361 agggcgtgct ttccgtggga cgcgtgcaga cgcccgtact cggactggtg gtgcgccgcg
    12421 acgaagagat tgaaaacttc gtggcgaaag acttctttga agtcaaagca catatcgtga
    12481 cacctgcaga tgagcggttt accgctatct ggcaaccgag cgaagcgtgt gaaccgtacc
    12541 aggatgaaga agggcgcttg ttacatcgtc cactggcgga gcatgtggtt aaccgcatta
    12601 gtggtcaacc agctattgtc accagctata acgataaacg ggaatcagaa tccgcgccgc
    12661 tgcctttttc gctttcggcg ttgcagattg aagcggctaa acgttttggt ctgagcgcgc
    12721 agaacgtgct tgatatctgc cagaagctgt acgaaacgca caagctaatc acttatccgc
    12781 gttccgattg ccgctatttg ccagaagaac attttgccgg acgccacgcg gtgatgaatg
    12841 ctatcagcgt tcatgcaccg gatctgttgc cgcagccagt ggtagatcca gatatacgca
    12901 accgctgttg ggatgacaaa aaggtcgatg cgcaccacgc catcattccg accgcacgga
    12961 gttctgcgat caacctgacg gagaacgaag cgaaggtcta taacctgatt gcccgtcagt
    13021 atttgatgca gttctgcccg gatgcggtgt tccgcaagtg tgttatcgaa ctggacattg
    13081 ccaaaggcaa atttgtcgct aaagcccgtt ttcttgctga agcaggctgg cgcacgctgt
    13141 taggcagcaa agagcgcgat gaagaaaacg acggcacgcc gctgcctgtg gtggcgaaag
    13201 gcgatgaatt gttgtgcgaa aaaggtgaag tggtagagcg gcaaacccag ccgccacgcc
    13261 attttaccga tgcaacactg ctttcggcga tgaccgggat cgcacgcttt gtgcaggaca
    13321 aagatctgaa aaagatcctt cgtgcgaccg atggtttggg gacagaagca acccgtgccg
    13381 ggattattga actgttgttc aagcgtggtt tcctgaccaa aaaagggcgc tatatccact
    13441 ccaccgacgc cggaaaagcg ctattccatt cgctgccaga aatggcgacg cgaccggaca
    13501 tgaccgcgca ctgggaatcg gtgctgacgc aaatcagcga aaagcagtgt cgctatcagg
    13561 actttatgca gccgctggtg gggacgcttt accaactgat tgatcaggct cgtagcactc
    13621 cggtcaggca gttcagagga attgtggcat cgggtggggg ggctgttaaa aaagcaccca
    13681 ataagcgtgt gaaaaaagcg gcaaagccca aaacagataa tacggattct gctaattaat
    13741 tcgttttcca ctcttaaata aagccaatgg tagatattct gccattggct ttattggtta
    13801 tactcgtcat gctttaagtt gcatgtgctg cgtctgcgtt cgttcagccc agtcacttac
    13861 tcatgtaagc t
//
LOCUS       DAZFRG010000121        13795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFRG010000121 DAZFRG010000000
VERSION     DAZFRG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.121"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..243
                     /locus_tag="ACHKUC_002914"
     CDS             <1..243
                     /locus_tag="ACHKUC_002914"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6619138.1"
                     /translation="GAVSRSRTLCLVDFGLRFNHKGRHYFKAANGEDIALSLSIGAAM
                     FNGHPDYERLIQIADEALYIAKRRGRNRVELCKASL"
     gene            268..2667
                     /gene="dosP"
                     /locus_tag="ACHKUC_002915"
                     /gene_synonym="pdeO"
     CDS             268..2667
                     /gene="dosP"
                     /locus_tag="ACHKUC_002915"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO6619139.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKVW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIECVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRIFALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(2857..3288)
                     /gene="osmC"
                     /locus_tag="ACHKUC_002916"
     CDS             complement(2857..3288)
                     /gene="osmC"
                     /locus_tag="ACHKUC_002916"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO6619140.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            3633..3848
                     /gene="bdm"
                     /locus_tag="ACHKUC_002917"
     CDS             3633..3848
                     /gene="bdm"
                     /locus_tag="ACHKUC_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO6619141.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            3950..4087
                     /gene="sra"
                     /locus_tag="ACHKUC_002918"
     CDS             3950..4087
                     /gene="sra"
                     /locus_tag="ACHKUC_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO6619142.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            4244..5941
                     /gene="maeA"
                     /locus_tag="ACHKUC_002919"
     CDS             4244..5941
                     /gene="maeA"
                     /locus_tag="ACHKUC_002919"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6619143.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASEMLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            6075..7085
                     /gene="adhP"
                     /locus_tag="ACHKUC_002920"
     CDS             6075..7085
                     /gene="adhP"
                     /locus_tag="ACHKUC_002920"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO6619144.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            7271..7549
                     /locus_tag="ACHKUC_002921"
     CDS             7271..7549
                     /locus_tag="ACHKUC_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6619145.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            7549..7833
                     /locus_tag="ACHKUC_002922"
     CDS             7549..7833
                     /locus_tag="ACHKUC_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6619146.1"
                     /translation="MKMVNHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(8062..8715)
                     /gene="fdnI"
                     /locus_tag="ACHKUC_002923"
     CDS             complement(8062..8715)
                     /gene="fdnI"
                     /locus_tag="ACHKUC_002923"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO6619147.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(8708..9592)
                     /gene="fdnH"
                     /locus_tag="ACHKUC_002924"
     CDS             complement(8708..9592)
                     /gene="fdnH"
                     /locus_tag="ACHKUC_002924"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO6619148.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(9605..12652)
                     /gene="fdnG"
                     /locus_tag="ACHKUC_002925"
     CDS             complement(9605..12652)
                     /gene="fdnG"
                     /locus_tag="ACHKUC_002925"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(12065..12067),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6619149.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            12884..13765
                     /gene="yddG"
                     /locus_tag="ACHKUC_002926"
     CDS             12884..13765
                     /gene="yddG"
                     /locus_tag="ACHKUC_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO6619150.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         3392 a         3561 c         3446 g         3396 t
ORIGIN      
        1 ggtgcagtta gtcgatctcg cacgttgtgt ttggttgatt ttggcctgcg gtttaaccat
       61 aaaggtcgac attattttaa agccgcaaac ggcgaggata ttgccctctc actttccatc
      121 ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc tcattcaaat agccgatgaa
      181 gctctgtata tcgccaaaag acgaggtaga aaccgtgttg aactctgtaa agccagtctt
      241 tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg atgcggataa tgccgccgat
      301 ggcatttttt tccccgccct tgagcaaaat atgatgggcg cggtgttaat taacgaaaat
      361 gatgaagtga tgtttttcaa ccccgccgca gagaagctct gggggtacaa acgtgaagaa
      421 gtcattggca ataacattga tatgctgatt ccgcgggatt tgcgtcctgc gcatcctcaa
      481 tacattcgtc acaaccgtga aggtggtaaa gctcgcgtcg aggggatgag tcgggagctg
      541 cagctggaga aaaaagacgg cagtaaagtc tggacccgtt ttgcgctatc gaaagtgagc
      601 gccgagggga aagtttatta cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa
      661 aaagaacaga cccgacaatt gattattgcc gttgaccatc tcgaccgacc ggtgattgtc
      721 ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac
      781 tgcattaacg aagccagcgg tatgcagccc gatacactcc tgaacattcc agaattccct
      841 gctgataacc gtattcgttt acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa
      901 tttctgctgt tgacgcgcac cggtgaaaaa atctggatta aagcctctat cagcccggtt
      961 tatgacgtgc tcgcgcatct gcagaacctg gtaatgacat tctcggatat cacagaagaa
     1021 aggcaaatcc gccagcttga aggcaatatt ctcgccgcca tgtgcagcag cccgccattt
     1081 catgaaatgg gggaaatcat ttgtcgtaac atcgaatgtg ttctcaacga atcgcatgtt
     1141 tcgctgttcg cgctgcgcaa cgggatgccg atacactggg cgtcatcttc gcacggtgca
     1201 gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc agcgcgatgg cgcgcctgcg
     1261 gggatcctgc aaattaaaac ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg
     1321 gcagatatca gccagcatat ggccgcgctg gcgctggaac aggaaaaaag ccgtcagcat
     1381 attgaacaac tcatccaatt tgatccgatg accggtctgc caaatcgcaa taacctgcac
     1441 aattacctcg atgacctggt cgacaaagcc gtctctccgg tggtgtatct catcggtgtt
     1501 gaccatattc aggatgtgat tgatagcctt ggctatgcgt gggccgatca ggcattgctg
     1561 gaagtggtca atcgctttcg tgaaaaactc aaaccggatc agtatctctg tcgtattgaa
     1621 ggtgcgtctt ttgtcctcgt gagcctggaa aacgatgtca gtaacattac ccagatcgcc
     1681 gatgagctac ggaatgtggt cagcaagccg ataatgattg acgataaacc attcccgctc
     1741 accttgagca ttggcatcag ctacgacgtg ggtaaaaacc gcgattacct gctctccact
     1801 gctcacaatg caatggattt tattcgcaaa aatggcggta acggctggca gttcttcagc
     1861 ccggctatga acgaaatggt aaaagagcgt ttggttttag gcgcagcgct aaaagaagcg
     1921 attagcaata accaacttaa actggtttat cagccgcaaa tcttcgcaga aacgggtgaa
     1981 ctgtacggca tcgaagccct tgctcgctgg tacgatcccc tgcatggtca tgtgccccct
     2041 tcacggttta ttcctctcgc agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc
     2101 atcgcggaag cttgccgtca gttagcagaa tggcgtagcc agaatattca tatcccggcg
     2161 ttatccgtta acttgtctgc gctgcacttt cgcagtaatc agctgcctaa tcaggtatct
     2221 gatgcaatgc acgcctgggg tattgacggc caccagctga cggtggaaat cacggaaagc
     2281 atgatgatgg aacacgatac cgaaatcttt aagcgcattc agatcctgcg cgatatgggc
     2341 atcggtttat cggtagatga ttttggtaca ggcttttccg gattatcccg cttagtcagt
     2401 cttccggtaa cggaaatcaa aattgataaa agttttgtcg atcgttgtct gaccgaaaaa
     2461 cgcatctttg ccttactcga agccattacc agcattgggc aaagcctcaa tttaaccgtc
     2521 gtggctgaag gcatcgaaac caaagagcaa tttgagatgc tacgcaagat ccactgtcgc
     2581 gttattcagg gatatttctt ttcccgcccc ctgcccgccg aagaaattcc aggctggatg
     2641 agcagcgtgt taccgctgaa aatctgataa attcctctcg cccgcactcg cgggttttta
     2701 tttaacgtga cactgtcact taaatactgt gatttccgct acgtttagtt ggacaacgac
     2761 aacgcatagc catgtcacaa caatcccttc tcagcctgat gtgattgtgc tgacgcatca
     2821 ggcattcacg cgcctgacgc gtcatccggc aacgctttac gatttcaact ggtaatccag
     2881 cgtaatttcc gctttcagta cctgagaaac cgggcatcct gcttttgctt tctggataat
     2941 gccgtcaaag gtggaggcat caatacccgg caccgcaact tcgctcttca gtgcgatttt
     3001 agtaatcgca aaaccggcat ccactttatc cagcgagaca tcagcggtgg tatcaatcga
     3061 tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga aacatgcggc
     3121 atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt
     3181 aaatccatac ggttgttggt tcagcacgcc gctctcagta gatactgttc ccttcccgcg
     3241 tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg ttgctctcct
     3301 gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata agcaggataa
     3361 tccgaataaa atccggcgaa aatataattc ctgcacttaa attaaatttc cacaatagat
     3421 aaccggaaat atataactca agagatattg catcaatatc aatcaactca tcacaataat
     3481 aatacctatc aatatcatgg agttaaaata taaaaatcat caaccaggac taatcttaac
     3541 aacgaagcgg caaatatttg ccatgctgaa tgtgctttat aaatctgcga tccgtagcag
     3601 acaccataaa tacacagaca cggagaatca ctatgtttac ttattatcag gcagaaaatt
     3661 caacagcaga acccgctctg gttaatgcca ttgagcaagg ccttcgggca gagcacggtg
     3721 ttgtcactga agatgacatt ctcatggagc tgaccaaatg ggtggaagct tctgataacg
     3781 acatcctcag tgatatctac cagcaaacga tcaattatgt ggtcagtggt cagcacccta
     3841 cgctttaagg tgctatgctt gatcggcaac ctaatttagg ggtttagcac gtgtttcttc
     3901 gctacggcga tgttgtcctt aaaactagca acaggattga ggagttaaaa tgaaatcgaa
     3961 ccgtcaggca cgtcatattc ttggactgga ccataaaatt tctaaccagc gcaaaatagt
     4021 taccgaaggt gacaaatcca gcgtggtaaa taatcccacc ggcagaaaac gccccgctga
     4081 aaagtaattc ataaccatca gtcctcaatg acgattaaac accattgcct gcgcaatggt
     4141 gtttttgttt ttatctgctt tatacttggt acttgacgcc ctggcggtaa tgcaaagacg
     4201 ataaaagccc ccagggatgg atattcaaaa aagagtgagt gacatggaac caaaaacaaa
     4261 aaaacagcgt tcgctttata tcccttacgc tggccctgta ttgctggaat ttccgttgtt
     4321 gaataaaggc agcgccttca gcatggaaga acgccgtaac ttcaacctgc tggggttact
     4381 gccggaagtg gtcgaaacca tcgaagaaca agcggaacga gcatggatcc agtatcaggg
     4441 attcaaaacc gaaatcgaca aacacatcta cctgcgtaac atccaggaca ctaacgaaac
     4501 cctcttctac cgtctggtaa acaatcatct tgatgagatg atgccagtca tttacacccc
     4561 aaccgtcggc gcagcctgtg agcgtttttc tgagatctac cgccgttcac gcggtgtgtt
     4621 tatctcttac cagaaccgcc acaatatgga cgatattctg caaaacgtac caaaccataa
     4681 tatcaaagtg attgtggtga ctgacggcga acgtattctg gggcttggtg accagggcat
     4741 cggcgggatg ggcattccga tcggtaaact gtcgctctat accgcctgtg gcggcatcag
     4801 tccggcctat acccttccgg tggtgctgga tgtcggcacc aacaaccaac agctacttaa
     4861 cgatccgctg tatatgggct ggcgtaatcc gcgtatcact gacgacgaat actatgaatt
     4921 cgttgatgaa tttatccagg ctgtgaaaca acgctggccg gacgtactgt tgcagtttga
     4981 agacttcgca caaaaaaatg cgatgccgtt acttaaccgc tatcgcaatg aaatttgttc
     5041 ttttaacgat gacattcagg gcaccgcggc ggtaacagtc ggcacactga tcgcagccag
     5101 ccgcgcggca ggtggtcagt taagcgagaa aaaaatcgtc ttccttggcg caggttcagc
     5161 gggatgcggc attgccgaaa tgatcatcgc ccagacccag cgcgaaggat taagcgagga
     5221 agcggcgcgg cagaaagtct ttatggtcga tcgctttggc ctgctgaccg acaagatgcc
     5281 gaacctgctg cctttccaga ccaaactggt gcagaagcgc gaaaatctca gtgactggga
     5341 taccgacagc gatgtgctgt cactgctgga tgtggtgcgt aatgtaaaac cagatattct
     5401 gattggcgtc tcaggacaga ccgggctgtt tacggaagag atcatccgtg aaatgcataa
     5461 acactgtccg cgtccgatcg tgatgccgct gtctaacccg acgtcacgcg tggaagccac
     5521 accgcaggac attatcgcct ggactgaagg taacgcgctg gtcgccactg gcagtccgtt
     5581 taatccagtg gtatggaaag ataaaatcta ccctatagcc cagtgcaaca acgcctttat
     5641 tttcccgggc atcgggctgg gtgttattgc ttccggcgcg tcacgtatca ccgatgagat
     5701 gctgatgtcg gcaagtgaaa tgcttgctca gtattcgccg ctggtcctga acggcgaagg
     5761 tctggtacta ccggaactga aagatattca gaaagtctcc cgcgcaattg cctttgcggt
     5821 tggcaaaatg gcgcagcagc aaggcgtggc ggtgaaaacg tctgccgaag ctctgcaaca
     5881 ggccattgat gataatttct ggcacgccga ataccgcgac taccgccgta cctccatcta
     5941 agcctgcgcc cggtagtgaa ggctaccggg caattttcct ctcccttttt cagatctcat
     6001 ccatactggg tagtggcgaa taaatctcat ttgcctcacc tgctatgcag aacatcatcc
     6061 gaaaaggagg aactatgaag gctgcagttg ttacgaagga tcatcatgtt gacgttacgg
     6121 ataaaacact gcgctcactg aaacatggcg aagccctgct gaaaatggag tgttgtggtg
     6181 tatgtcatac cgatcttcat gttaagaatg gcgattttgg tgacaaaacc ggcgtgattc
     6241 tgggacacga aggtattggt gtggtggcag aagttggccc tggtgttact tcattaaaac
     6301 caggcgatcg tgccagcgtg gcgtggttct acgaaggatg cggtcattgc gaatactgta
     6361 acagtggtaa cgaaacgctc tgccgttcag ttaaaaatgc cggatacagc gttgatggcg
     6421 ggatggcgga agagtgcatc gtggtcgccg attacgcggt aaaagtgcca gatggtctgg
     6481 actcggcggc ggccagcagc attacctgtg cgggggtcac tacctacaaa gccgttaagc
     6541 tgtcaaaaat tcgtccagga cagtggattg ctatctacgg tcttggcggt ctgggtaacc
     6601 tcgccctgca atacgcgaag aatgtcttta acgccaaagt gatcgccatt gatgtcaatg
     6661 atgaacagtt aaaactggca accgaaatgg gtgcagattt agcgattaac tcacgcaccg
     6721 aagacgccgc cagaattgtg caggagaaag ccggtggcgc tcacgcagcg gtggtgacag
     6781 cggtagctaa agctgcgttt aactcggcgg tggatgctgt ccgtgcaggt ggtcgtgtcg
     6841 tggctgtcgg tctgccgccg gagtctatga gcctggacat cccacggctg gtgctggatg
     6901 gtattgaagt ggtcggttcg ctggtcggca cgcgtcagga tctaaccgaa gccttccagt
     6961 ttgccgccga aggcaaagtg gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca
     7021 ccatctttac cgagatggag gaaggcaaaa tccgtggccg tatggtgatt gatttccgtc
     7081 gttaagaggt ctttgctgcg actgccatgt tcgggtcgca gcctgttttt cgagacgctt
     7141 tccgcaattt cactcttctt cttttcctcc cccatctttc ccctcacaat tcgcgaaaac
     7201 gtagcgaaat ttgttgcgct gcacggaaca atagtgatag tgttcctttc gagggaacaa
     7261 ggatgaagag atgataatga atttcagaca caagggattg cgtgacttgt ttcttctcgg
     7321 caaaacctcc ggcgttatac cgacgcaagt caaacgatta cgccaccgac tcgctgtgat
     7381 tgatgcagca tgctgtcttg ctgatatcga tatgcccggt taccgactac atccgttaag
     7441 cggcgatcgc gatggaattt gggcgatatc tgtctcgggc aactggcgaa tcacatttga
     7501 attcgtcaat ggcgatgcat acatactgga ttacgaggac tatcactgat gaaaatggtc
     7561 aatcatcccc gcccggggga cattattcag gaatcactgg acgaacttaa tgtcagcctg
     7621 cgcgagtttg ccagagcaat ggaaattgcg ccctcaacgg caagccgatt gctgaccgga
     7681 aaagcagctt tgacgccaga aatggcaata aaactttccg tggtgatcgg cagttcgccg
     7741 caaatgtggc tgaatctgca aaatgcctgg agtctggcag aggcagaaaa aacagtggat
     7801 gtgtcgcgac tgcgccgtct ggttacgcaa taaaaaaatg gcaccttata aagcgccatt
     7861 tgaaactgat gacaaacgca acattgcccg atgcgctacg cttatcaggc ctacacgttc
     7921 actgcaatct cctggatttg aacggtttgt aggccggata aggcgttcac gccgcatccg
     7981 gcatgaacaa agtgcacgtt gtctacaata aaaaaatggc gccttttaca gcgccatttt
     8041 cagcttaaag ctcaaaagag attataaccc ttcttcactc tcttttttcg cttctgcctt
     8101 ctcgatttca cgataccagc gcggatggtg tttcttcgcc cagcgacggc tcaccttccc
     8161 ttcgatcatc cctttaatag atcctttcac ccagaatgcc atatacatat ggatcaggat
     8221 ggcgtggatc aggatgatac ccgcagccgc atggatcagc aggctataac ggacaacctg
     8281 tattgggaag tactgcgcaa agtacggacg ccagataatc actccggtca ccagcagcac
     8341 gaaaatcatg ctcatgattg accagaacat catcttttgc ccggcgttgt acttaccgac
     8401 atccgccact ttgtgctcat tgccttttaa tacttcgaca atgttcaaca gccacggaat
     8461 atctttctta tccgggatgt tgtgatgcac aaaacgcaca aacataaaca tcagcgcgac
     8521 gaaaatcgca atgccgaaga acgggtgcaa aatgcgtccc atctgcggcg taccgaaggt
     8581 ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc ccggacagcg ccaccaggaa
     8641 gaagcaaatc accacggtcc agtgacaggc gcgatcaata aatttggtgc gcacaatcat
     8701 tttcgactta ctcatgattt ccctcctcgt catcgtccac ttccttattc gggccaatac
     8761 cgatgtagtg gaaaatcaac ccggcaaaag tggcaataaa gccagccgct gccagcggtt
     8821 tcaacgcgcc tttccacagg cttaccgagg tatcgatctt cggatctttc ggcagaccat
     8881 gatacagctc cggctgatcg gcatgatgca gcacgtacat aacgtgtgta ccaccgaccc
     8941 cttccgggtt gtagacgcca gcatgttcgt aaccacgcgc tttcagtttc gctacgcgct
     9001 gttccgccag ctccagcatc tccttcttgg tgccgaagtg gatagccccg gtcggacagg
     9061 ttttcacaca agccggttcc tggccgacgc tgacgcgatc gacgcagagc gtgcatttat
     9121 atacccggtt atcctctttg ttgaggcgcg gaatattaaa cggacacccg gcaatgcagt
     9181 aaccacagcc gatgcaattt tccgactgga aatcgacaat cccgttagcg tactgaatga
     9241 ttgcaccagc agacgggcac gccttcaggc agcccggatc ttcacagtgc atacagccgt
     9301 ctttacggat cagccactcc agcttgccgt tctgttcggt ttcgctaaag cgcatcaccg
     9361 tccaggactt ggcgctcaga tcggcggggt tatcgtaaac cccgacgcag tgccccactt
     9421 catcacggat gtcgttccac tccgaacacg ccacctgaca ggctttacag ccgatacagg
     9481 tggaaacgtc gataagtttc gctacttctg ctttgtaatc acgcacctga gaaggcggcg
     9541 tgatggagtt agttgcggac cttttgataa tgtcctgcgt ttccatagcc atctgttcgc
     9601 ccccttacgc cttctcgatg ttgactaaga acgctttata ttccggcgtt tgcgagtttg
     9661 catcaccgac attcggcgtc agagtgttag cgatataacc tttacgcgcg acaccctcaa
     9721 agccccagtg gattggaata cccaccgttt caacctgctg accgtttaca ttcagcggtt
     9781 tcagacggcg cgttaccaca gccaccgcgc ggataaagcc acgcttgctg gagacagtga
     9841 cacgatcgcc attggcaatg cctttcgccg ccgccagcgt ttcgctgatt tccacaaact
     9901 gttccggctg agcaattgcg ttgagcaatg cgtgcttggt ccaggtgtgg aagtgctccg
     9961 tcagacgata ggtcgtaccc acatacggga actgctcttt tttacccatc cgcagcgcgt
    10021 cttgttcata cagacgaaca accgggttag acaccacgtt cggatgcaac gggttagtgc
    10081 ccagcggcgt ttcaattggc tcgtagtgtt ccgggaacgg accttccgcc attttgttga
    10141 tggcaaacag gcgtcccatc ccttccggct gcatgataaa cggcccggtt ggcgtacccg
    10201 gtgcggcatt gccgaagtcc ggaatatcgt tacccgtcca cttgctaccg ttccactgga
    10261 tcagcatccg tttcggatcc cacggtttac cgttgatatc cgccgaagca cggttgtaga
    10321 gtacgcgacg gttgagcggc cacgcccagg cccatcccag cgtattcccc agaccggacg
    10381 ggtcggagtt atcgcggtta gccatctggt tgccctgctc ggtccagcta ccggtgtaga
    10441 tccagcaaga agatgcggtt gtaccgtcgt cacgcagatg cgcaaagcta ctcagcaact
    10501 gacctttctt cgcaatcagc acgccattag cgtcatagag atcttccagc gcatagccgt
    10561 tgttctcttt cgccacttcg tcagactgcg gttcgtgcgg ctgcttgtag ttccagctca
    10621 tcttcatcag cggttctacg cctttaccac cctcggcctg gtacagctcg cgcagatgat
    10681 ggtagatacc cgccagaatt tcgccgtcgt tacgtgcttc gcccggcgcg tcctgacctt
    10741 tccagtgcca ctgcaaccag cgaccggagt tagcgataga accatcttct tcagcaaagc
    10801 aggtcgaagg cagacggaat acttcagtct gaatagatgc cggatcgaca tcgttcgact
    10861 cgccgtggtt ctgccagaag gtagaggttt cagtcaccag cggatcgata accaccatgt
    10921 acttcagttt gctcaggcag ctcaccactt tgtttttgtc cgggaaggac gcaaccgggt
    10981 taaagccctg gcagaaataa ccggtgactt tgccttcgtc catcatgttg aaatacttga
    11041 tgacgtcgta ggtctggtcc cacttcggca gccagtcgta gccccagttg ttctctttct
    11101 gcgcggcatc gccgtagaaa gatttcatca ggctaacgaa gaacttcgga tagttgctcc
    11161 agtagttcac ctgatcagcc agcgtcgctt tcggcgtatt cgcttccaga tacgactgca
    11221 aatcaacctg tttttctgac ggtagcgtca gataacctgg caggctggta gagagcaggc
    11281 ctaagtcagt caggccctga atgttggagt gaccacgcaa tgcgttcacg ccgccaccgg
    11341 ccatacccat gttaccgagc agcaactgga tcatcgccat cgtacggatg ttctgcgcac
    11401 ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt ggttgtgcga tccggtgcgc
    11461 tggtggaggc cagcacttca cacactttca ggaagtcggc ttttggcgta ccgcaaatgt
    11521 tttctactac gtccggcgtg tagcgggaaa cgtgcgcttt cagcagattc catacacagc
    11581 gcggatgagt cagcgtgtca tcgcgtttcg catagccgtt ttcatcgaac tgatagttcc
    11641 aggacgactt atcgtattga cgtttttcag cgtcgtagcc gctgaacaga ccgtcttcga
    11701 aagcaaaatc atcacgcacc agcaggctgg cgttggtgta atgcttaacg tattcggcgt
    11761 tgattttgtt gttttcgatc aggtagcgca aaacgccaga caggaacgta atgtccgtac
    11821 cggaacgaat aggtgcgtaa atatccgcca cagaagcggt acgcgtaaaa cggggatcga
    11881 caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat cgcccagcgg aaaccgacgg
    11941 gatgcgcttc agcagcgtta ccgcccatca ccattacgac gttagcgttt ttgatatcca
    12001 cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc aagacttgct accgttggtc
    12061 cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc gagggagcgg gcaaattttt
    12121 gagtcagcat cccggtttcg ttgctggcac cggaagcaca cagcataccg gtagaaagcc
    12181 aacggtttac cgttacgccc tgctcgttct tttcaataaa gttagcgtca cggtcggctt
    12241 tcatcagctt cgcaatacgg gagaatgctt cttcccagct aatgcgctgc catttgtcag
    12301 aacctggcgc acgatattcc gggtagcgca gacggttttc gctgttgacg taatccagca
    12361 aaccggcccc tttcgggcac agcgcaccac ggcttaccgg atgatccggg tcaccttcaa
    12421 tgtgataaat cgcttctctg gcgtttttcg cgccatcacc caggctatac atcaatagcc
    12481 cgcaacctac ggaacagtat gtgcaggtgt tacggatctc tttcgcgcgt aataatttgt
    12541 agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc caatgctgct actgttgttc
    12601 ccgccatacc gcccgcgcag attttaaaaa attgtctgcg actgacgtcc attgctttcc
    12661 tcttttttca ggggtattac tgcgcgagga aactaacagt aaaattctga atctttttgc
    12721 gcgaaatcaa aaagaacacg tgtttgccac aaaataatcc gcctgcgcaa attgacctac
    12781 ctcaatagcg gtagaaaaac gccccactgc ctgacagggc agttaaaaaa atgctataaa
    12841 attcagctta atttttaacg gcaagagaga caaaacagcg agcatgacac gacaaaaagc
    12901 aacgctcata gggctaatag cgatcgtcct gtggagcacg atggtaggat tgattcgcgg
    12961 tgtcagtgag ggactcggcc cggtcggcgg cgcagctgct atctattcat taagcgggct
    13021 gctgttaatc ttcacggttg gatttccgcg tattcgacag atcccgaaag gctatttact
    13081 cgccgggagt ctgttattcg tcagctatga aatctgtctg gcgctttcct tagggtatgc
    13141 ggcgacccgt catcaggcga ttgaagtggg gatggtgaac tatctatggc ccagcctgac
    13201 aattctcttt gccattctgt ttaatggtca gaaaaccaac tggctgattg taccaggatt
    13261 attattagcc ctggtcggcg tctgctgggt gttaggcggt gacaatgggt tacattatga
    13321 tcaaattatt aataatatca ccaccagccc attgagttat ttcctggcgt tcattggggc
    13381 gtttatctgg gcagcctact gcacagtaac gaataaatac gcacgcggat ttaacggaat
    13441 taccgttttt gtcctgctaa cgggagcaag tctgtgggtt tactattttc ttacgccaca
    13501 accagaaatg gtatttagca cgcccgtcat gattaaactc atctctgcgg catttacctt
    13561 aggatttgct tatgctgcat ggaatgtcgg tatattacat ggcaacgtca ccataatggc
    13621 ggtaggttcg tattttacgc ccgtcctttc ctcagcgctt gcagccgtgc tgctcagcgc
    13681 cccgctgtcg ttctcgttct ggcaaggcgc gctaatggtc tgcggcggtt ccctgctctg
    13741 ctggctggcg acacgtcgtg gctaaaataa ttgtcgggtc tacggcccgg cagtt
//
LOCUS       DAZFRG010000122        13787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFRG010000122 DAZFRG010000000
VERSION     DAZFRG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.122"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..1177
                     /gene="tssD"
                     /locus_tag="ACHKUC_002927"
     CDS             44..1177
                     /gene="tssD"
                     /locus_tag="ACHKUC_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6619151.1"
                     /translation="MSDEIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDNNGLKTLNSKAVGRLLAAGGIYNGNIEGFRKTANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFNPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1174..1431
                     /locus_tag="ACHKUC_002928"
     CDS             1174..1431
                     /locus_tag="ACHKUC_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6619152.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1506..1751
                     /locus_tag="ACHKUC_002929"
     CDS             1506..1751
                     /locus_tag="ACHKUC_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6619153.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1920..4181)
                     /gene="metE"
                     /locus_tag="ACHKUC_002930"
     CDS             complement(1920..4181)
                     /gene="metE"
                     /locus_tag="ACHKUC_002930"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO6619154.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4299..5252
                     /gene="metR"
                     /locus_tag="ACHKUC_002931"
     CDS             4299..5252
                     /gene="metR"
                     /locus_tag="ACHKUC_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO6619155.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5140..6039)
                     /gene="bioP"
                     /locus_tag="ACHKUC_002932"
     CDS             complement(5140..6039)
                     /gene="bioP"
                     /locus_tag="ACHKUC_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO6619156.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6115..6915)
                     /gene="yigL"
                     /locus_tag="ACHKUC_002933"
     CDS             complement(6115..6915)
                     /gene="yigL"
                     /locus_tag="ACHKUC_002933"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO6619157.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPEII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6923..7945)
                     /gene="pldB"
                     /locus_tag="ACHKUC_002934"
     CDS             complement(6923..7945)
                     /gene="pldB"
                     /locus_tag="ACHKUC_002934"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO6619158.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLGDP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8056..8676
                     /gene="rhtB"
                     /locus_tag="ACHKUC_002935"
     CDS             8056..8676
                     /gene="rhtB"
                     /locus_tag="ACHKUC_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO6619159.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8737..9357)
                     /gene="rhtC"
                     /locus_tag="ACHKUC_002936"
     CDS             complement(8737..9357)
                     /gene="rhtC"
                     /locus_tag="ACHKUC_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO6619160.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9421..11250)
                     /gene="recQ"
                     /locus_tag="ACHKUC_002937"
     CDS             complement(9421..11250)
                     /gene="recQ"
                     /locus_tag="ACHKUC_002937"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO6619161.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11375..12244)
                     /gene="pldA"
                     /locus_tag="ACHKUC_002938"
     CDS             complement(11375..12244)
                     /gene="pldA"
                     /locus_tag="ACHKUC_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO6619162.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12409..12876
                     /gene="yigI"
                     /locus_tag="ACHKUC_002939"
     CDS             12409..12876
                     /gene="yigI"
                     /locus_tag="ACHKUC_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO6619163.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNKPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12928..>13787
                     /gene="rarD"
                     /locus_tag="ACHKUC_002940"
     CDS             12928..>13787
                     /gene="rarD"
                     /locus_tag="ACHKUC_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO6619164.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDA"
BASE COUNT         3476 a         3595 c         3417 g         3299 t
ORIGIN      
        1 gtttattttt aaaacatcaa gcaataaaga gagttcatca aagatgagtg atgaaattta
       61 cctgaccata accggagaac aacaaggatg tatttcctct cgttgcggaa cgagcgcatc
      121 aataggtaat cgctggcaga ttggccatga agatgaaatc ttcgcgttct cgctatctaa
      181 tagtattacg aatacaggca aagggtcaca attacatggc cttagtttct gtaagttgat
      241 tgacaaaagc tccccgttac ttattaacgc tattaacaat aacgagcagt tgttcatgga
      301 gtttgacttc tatcggatca atagatttgg tcgatgggag aaatattaca acattcaact
      361 ccgcggcgcg ctcttatcag caatcaatca cctgtttact gagaacaatc tggatacaga
      421 aaccatcact gtcagttatg aatatatact ctccaggcat ctgattgcta atactgaatt
      481 tagtcatttg gcatttcccg ataactacaa ccgtctgttt ataccacggc aaaaaactaa
      541 agataataat ggcctcaaaa cactcaatag taaagcggtt ggcagactgc ttgctgctgg
      601 cggcatatac aatgggaata tcgaaggatt caggaaaaca gcaaacaagt tgggaggaga
      661 tgcacctgct ggttacgatc aagttatgga taacaaaggg ttactcatcg ctggtgcttc
      721 aatagttgct ggagttgttc tgggaagaat gagattccct gatttggaat catttgagca
      781 tttcggagcc agaggagcag tatcaggaag accatttaat cccgaccttg ccggtggtcc
      841 aatagaaaac ctgacgatag atggcgtaac aatcaatagg gaaggtatcg ctattgttga
      901 aaagcatatc tccagatttg atcatgatcc agctaatgat gtaatgattt caaggctaaa
      961 aaaaatagca aataaagaat tgcttcctga aaaatacgat ctgaactact atactcatga
     1021 atgccgcgag tatcagcgtt attgtaattt aggatgggaa acaggtgaac cgaatggttt
     1081 agatggatat gaattatgga ataatgcaca tactgcaact ctagaagact ttaaaattaa
     1141 agatactgac ttatttcatc cggatgcaaa aaaatgacga ttacgatagt tgaatttaac
     1201 gtgctgaaag ttatggctga gaaagatatt gactggagct ggatggtgct ggatcgtact
     1261 ttagctatac gaaatattcc aggatttggc aacgtagcga atatcgtaac aaagctggtt
     1321 aatcatgggt tggttgatat cgtaaatggt gagggcaact ctaaaccacg ctacagagtt
     1381 tctcaatatg gattgaatct tatcaaagaa caacaagaca acctcttctg atccagccat
     1441 tacaggagca tgatttacat tgtgatcttt caataagata ctcatcaatt ttggtacaga
     1501 atatcgtgat cactaaaaat gaacttaacg tgctgaacgt tatgactgag aaagatataa
     1561 actggaactg gatgaatctc gatcgcacac tatccataag gcaaatacca ggattcggta
     1621 atgttgttaa tattgttaac aaattagcta acgaaggttt ggttattatt gaggatagcg
     1681 ggcataagtc tatgcctcac taccacgtta gtgaaaaggg atacaatctc gtgcaacgtg
     1741 agaataaata acgtagcgtt gattactgaa cggaatcact caaaactgtg ttttgcgaaa
     1801 aaaacgattc ggttatttct actcctgaag atctgcttca atctggcact catgcaaaat
     1861 ggcttccagt actggaagcc attatcgtac tgcaatttac aatgactcgt taatactcat
     1921 catccccgac gcaaattctg cgccgcctgc accatgttcg ccagtgccgc gcgggtttct
     1981 ggccagccgc gcgttttcag gccacagtcc gggttgaccc acagacgctc tgccggaatg
     2041 cgttttgccg ctttcttcag caaggcttca atccattcca cgctcggtac atttggcgag
     2101 tgaatgtcat agacgccagg accgatttca tttggataat caaactcttc aaacgactcc
     2161 agcaactcca tgtcggaacg cgaggtttcg atggtgatga cgtctgcgtc caacgccgca
     2221 atcgaatcca tgatgtcgtt gaactcgcag taacacatgt gagtgtggat ttgtgtgtca
     2281 tctttcgcca cggcggcgtt gatacggaaa gcctccacgc cccactggag ataggcatcc
     2341 cagtcgctgc gacgcagtgg taaaccttcg cgcaatgccg gttcgtcaat ctgaatgatg
     2401 ccaattccag cggcttccag gtccgcgact tcatcacgca gcgccagcgc aatttgtttg
     2461 gcgatggttt cacggctgac atcttcacgc gggaacgacc agcagagaat agtcaccggg
     2521 ccggtcaaca tccctttcac cggtttatca gtcagggatt gcgcatattt tgcccactcc
     2581 acggtaatcg gcgccggacg actaacgtca ccaataacaa tcggtggctt cacgcagcgg
     2641 gaaccgtagc tctgtaccca accgttttgc gtaaagacaa acccgtcgag gtgctcgcca
     2701 aagtattcca ccatgtcatt acgctcggcc tcgccatgta ccagcacatc cagtcccaaa
     2761 cgttcctgct caacaatggc ctgcttgata tgttccgcaa tgcccgtacg gtagttattg
     2821 gcgtcgagat tacccttttt gaaatccaga cgcagggtac gaatctccgt ggtttgcggg
     2881 aaggaaccaa tcgtggtggt cggccacgcg ggcagtttaa aacgcgcacg ctgggcttca
     2941 gcacgcactt catagacatt cgcacgctga ctgtcctgag cggtgatcgc cgccagacgc
     3001 ttttctactg ccggattatg tacacgagta gagtgacgac gcgcctgaat cggagcgctc
     3061 cactctgcca gagctgccgt atcaccactg ttcagcgcat cgcgcagcaa tgccagttca
     3121 tgacattttt gcagggcgaa ggcaaaccag cttttcactt ctgcatcaag acgcgtttcc
     3181 acgctcaggt cgatggggct gtgcagcaac gagcaggaag atgccaccca caaatcgcgt
     3241 ttgccgacaa tgtccttaat ttgcgcatat ttctcggtaa gatcggcgcg ccagacgtta
     3301 cgaccattga taagacccgc agacagcagc cagtcagaag gcagacgctt gtgcagttca
     3361 gcaacgtcat ctttaccatg tacgagatcg acatgcagac cctgaacagg cagcgcagta
     3421 atcgtgtcga ggtttggcgt tacgccttca aaataggtag tcagcagcag tttcacctgc
     3481 ccctgcagag catcgtaagc gggtttgtac gcatccagcc acgcctgcgg cagttccaga
     3541 accagcgcgg gttcgtcgat ctgtacccac tcaatgccgc gtttcgccag ttctgccagc
     3601 acttgctgat aaaccggcag aatgtcgttc agcaggctaa ggcggtcaaa ctgttcacct
     3661 ttcactttac ccagccacag gtaggtaatc ggccccagca gcacaggttt caccttgtgg
     3721 cccagcgcca gcgcctcgtc cacttcctcc agcaactgcg tccaggtcag tttgaactgt
     3781 tggcctttaa cgaactccgg caccatgtag tgatagttgg tgttaaacca tttggtcatt
     3841 tccgctgccg ccgcaggttc gccagtcggt gcacgtccac gaccaatacg gaacagggtg
     3901 tcgatatcta ccgaaccatc tttgttctgg tgacgcgccg gaacgttacc cagcagcagg
     3961 ctggtggtca ggacatgatc gtaccaggca aaatcgccca ccggcagcag gtcgataccc
     4021 gcttgctttt gttgatccca gtgacgagca cgcaattcac gccctaccgc cagcagttct
     4081 tcacgcgtgg agttccccgc ccaataactc tcttgcgctt ttttcagctc gcgacgcagg
     4141 ccaacgcgag ggaaaccgag ggtgtgatta agaattgtca ttatatgccc cttatggaat
     4201 ttttagtatt tagccgtcca gatgtttaca catccataat tggcggttac tgtatattcc
     4261 tcaagcgcaa attattcatg ccgaagtgaa ggactttcat gatcgaagta aaacacctga
     4321 aaacgctaca agcgttgcgg aactgcggct cactcgcagc cgctgcggcg acgttgcatc
     4381 aaacgcaatc cgccctgtct caccagttta gcgatctgga acaacgcctt ggcttccggc
     4441 tatttgtgcg taagagccag ccgctacgct ttacaccgca gggagaaatc ctgttgcaac
     4501 tggcaaacca ggtactgccg caaattagcc aggcactgca agcctgcaat gaaccgcagc
     4561 agacgcgtct gcgcattgcc attgagtgcc atagctgtat tcagtggctg acgcccgcgt
     4621 tagaaaattt ccataagaac tggccgcagg tggagatgga ttttaaatca ggcgtaacgt
     4681 ttgacccgca gcctgcattg cagcagggcg agctggatct ggtgatgacc tccgatattc
     4741 tgccgcgcag tggcctgcat tattcgccga tgttcgatta tgaagtgcgt ctggtgttgg
     4801 cacctgacca tccactggcg gcgaaaacgc gtattacacc ggaagatctc gccagcgaaa
     4861 cgctgttaat ttatccggtg cagcgtagcc gactggatgt ctggcgccat tttcttcagc
     4921 cagcaggcgt tagcccgtcg ctgaaaagcg tcgataacac cttgttgttg attcagatgg
     4981 ttgccgcgcg gatgggtatt gccgccctgc cgcactgggt ggtggagagt tttgagcgcc
     5041 agggtctggt cgtaaccaaa accctgggcg aaggattgtg gagccgactg tacgccgccg
     5101 tgcgcgatgg cgagcagcgt cagccgatta cagaagcgtt tattcgctca gcgcgcaatc
     5161 acgcctgcga tcatctgccg tttgtgaaga gcgcggagcg acccacttac gatgcaccca
     5221 cagtgaggcc aggatcacca gcgcgcctgt aataaacgtt ggccagtgcg gctgttggtg
     5281 ccagatagcc aggttaacca acagccctgc cggaacgtgc atattattca taatgcccag
     5341 cgttccggcg tccacctgcg tcgcgccgta gttccacata aagtagccaa taccggaagc
     5401 caccacgcca agaaacacca gaatgcccca ttgcagcgtg gtttgcggca ttttctgcgc
     5461 atttcccagc aagaaccatg caatcactgc caccaggaac gcgccaagat agaaccacgc
     5521 aaaggcgtta tgctgcggca tcgggcgagt ttccatcaga cgtttgtaac ccaccatgcc
     5581 gatggcaaaa gtgatattgg agagctgcac cagcaacaag ccagtccaga aatggtcggt
     5641 gacctgatca tagcgaataa tcccggcacc aatcaccgcc aacaaggcgc taaaagcata
     5701 gccccagcgc agacggcgct tactcatgat gtcataaatc agcgtgatgt agagcggcgt
     5761 cagtacggtg aacagcaaca gttcggagac cgtcaggtag agataggcgc ggaaactcag
     5821 catatacatc acaccgagct gcatcgcgcc caccagcata tacaggccaa ccgttttcag
     5881 gctattgcca cgggtgcgca gaaacggcag aaaaacgagt gccgccaggc caacgcgcac
     5941 cagcaccgca aaatagctat cgacgtgccc cgcaaggtac tcgccataaa agctaaagga
     6001 gaaggcccac agaatcgtgg tgatgataag tagcgccaca atggaaaact ctttgattaa
     6061 cgagtatcca ttgtagcgaa gtatcaggtt gacaactgac caaataaaga acgattacga
     6121 caaatagagt ttacgcagat aatgcggcac tgcgtcttcg gcgttagtac caatcacttc
     6181 cagctctgga tgcagatctt tcagccgttg atgcgcgctg cccataatgc agcctttccc
     6241 cgccatcgac agcatttcgg catcgttcat tccatcgcca aaggcaatac aatcttgcag
     6301 gctgtagccc agtttcttcg ccaccgcttc cagcgcatgg ccttttgaaa cgccgcccgc
     6361 catcacttcc aggcaggtta aggtagagaa actgacattg acgcgatcgc cccagcgagc
     6421 gttaatcgcc tgctccagtg gcagaagttt ttcatgggaa tcgcaggtga agaacacttt
     6481 gctgacgcct tccggctcca gcaatcccgg ctcatacagc gcataattga acaccgcttc
     6541 tttgaaaaag cgcatctctt ccgggcgatg gcgattcata aaccattcat catcgcgata
     6601 aacgttagta atgatctccg ggttgtcgtt gaccacgcca aacaggtcac tggcaatgtc
     6661 gcgatccagg ttatgagcaa aaatcaggtt gccatccaga tcgtgaacgc gcgcgccatt
     6721 ggaggtaatc atgtaagact taatctccag attatcgcga atttgcccca catcaacgtg
     6781 gtgacgaccg gtcgcaaaca caaagttgat gccgcgcgcg gtgagcagct tcagagtttc
     6841 tttggcgtaa ggggataacg tatggtcggg agaaagtaac gtgccatcta aatcagacgc
     6901 aacaacctgg tacataagaa atttaaacct ctgtagagcg gtttccgctg ggtgagttat
     6961 gcctgttgaa aaaatcaacg atagcgtgga gcgcgactga gcgcattgcg tccttttcaa
     7021 aaaggatctc atggtacgca cctttaatta ccaacggccg tcctccttcg acaggatggc
     7081 ccgcagcggt gcgtagttca caaaaacggt cgtgcatgcg gttatccacc acgcgttcct
     7141 cttcagcttg taagagaagc gttggcgtgg cgtcatcacc cgcaccggct aacacctgtt
     7201 cgccagccag aatactttcg cgtacccaat ggtaggtcgg cccaccgacg cgaattgttg
     7261 ggtcatcagc atagaaacgt aagttacgtc gatatcgctg tctgctgtgg gtcagtacgt
     7321 tgatagcaaa cggcaacgcg cgccagcgcc cggtgcctat tgcatagcca tcacggaaac
     7381 gtggatgcgc ttcggcccaa ttgaggatct gccgtgccat aaatgacggc atacgaatca
     7441 cgatcccaaa cattggcgca gttagcgcaa tggcgtcaca tacacctgga tggcgttgca
     7501 gaaataatgt ggagatcgca ccgcccatcg aatgtgccag tatatagcgt ttacgccacg
     7561 gaccgggctg aacctcctgt tgccagaatg ccgccagatc atcaacataa tcattaaagc
     7621 gattaacatg cccgagatgt ggatcgccta acaggcgacc ggaacgtccc tgcccgcgat
     7681 ggtcgatgat taaaacatca aaccccaaat ggaacaggtc gtaggccagt tccgcatatt
     7741 ttacgtagct ctcaatacgc cccggacaga tgactaccac ccggtcatgg tgctgtgcgc
     7801 gaaaacggac aaagcgcacc ggaatgtcat ccacaccagt aaactctgct tcatcacgct
     7861 gacgccagaa atcagtcagc ggtcccatgg taaaagcagc aaacgcgttt tctcttgttt
     7921 cccagtcttt ttgctgctga aacatcgggt aatctgcctc ttaaaccacg taaaatcgtt
     7981 tttttaagcg tgcctgacac aacgctgcga cagtagcgta ttgtggcaca aaaatagaca
     8041 caccgggagt tcatcatgac cttagaatgg tggtttgcct acctgctgac atcgatcatt
     8101 ttaagcctgt cgccaggctc tggcgcaatc aacaccatga ccacctcgct caaccacggt
     8161 tatcgcggcg cggtggcgtc tattgctggg cttcagaccg gactggcgat tcatattgtg
     8221 ctggttggcg tggggttggg gacgctattt tcccgctcag tgattgcgtt tgaagtgttg
     8281 aagtgggcag gcgcagctta cttgatttgg ctgggaatac agcaatggcg cgccgctggc
     8341 gcaattgatc ttaaatcgct ggcctccact caatcgcgtc gacatttgtt ccagcgcgca
     8401 gtttttgtca atctcaccaa tcccaaaagt attgtgtttc tggcggcgct atttccgcaa
     8461 ttcatcatgc cgcaacagcc gcagctgatg cagtatatcg tgctcggcgt caccactatt
     8521 gtggtcgata ttattgtgat gatcggttac gccacccttg ctcaacggat tgctctatgg
     8581 attaaaggac caaagcagat gaaggcgctg aataagattt tcggctcgtt atttatgctg
     8641 gtgggagcgc tgttagcatc ggcgaggcat gcgtgaaaat aatgtcggat gcggcgtaaa
     8701 cgccttatcc gacttactct gatgacgcgt ctggcattaa cgcgaaataa tcaaatgaat
     8761 gccaaatccg gcaaataacg ccccggcaaa accatcaatc cacttcgcca gacgttgata
     8821 accacggcgc atttgcggca gagcaaacag gctggcaacg acggtaaacc acgccagtgt
     8881 ttcgacaata atcaacgcga aaatgcccca gcgttcggtg gtgccgacat tatcaccgac
     8941 aaacagcgag aacaccgaac caaagtagat aatcgcttta gggttcgcga gattggtcag
     9001 taagccctta agaaaactgc gcccgctttt cgccagttcg acttgtggcg caggggcaga
     9061 aacaacctct tttttcagtg caccacgtag catctggtaa cccatccagc agagatacag
     9121 gccaccgccc accataatca gcgtatgcag ccaggccatt ttttcgataa tcaaatgtag
     9181 gccaagcaac gcaatcccag cccaaaccat tacgccgcag gtaatgccca gcacgcccat
     9241 catcgcttct ttacgggaac gactaacagc ggtctgagag acaaaaaaga aatcgggacc
     9301 ggggctcata agcgccacaa tgtgcaccat ggcgacggtg aggaataaca tcaacataca
     9361 ttagactcgc gggggaaacg tttacggagt cttcatcctg gcactttttt atgctgctga
     9421 ctactcttcg tcgtcaccat cgacatgcgc acgaatcagc gccataaacg gtttgccaaa
     9481 gcgttccagc ttgcgcatac caacgccgtt aacgctgagc atttcgctgg cggtgatcgg
     9541 catctgctcc gccatctcaa tcaaggttgc gtcgttaaac accacgtacg gcgggacatt
     9601 gctttcatcg gcaatcgatt tacgcagttt gcgtaatttg gcgaacagtt tgcgatcata
     9661 gttgccgccg aacgatttct gcatcgcttt cggtttgagc gccacgatac gcggcacggc
     9721 aagttgcaaa gaggattcgc cgcgcagcac cggacgcgca gcctctgtca gttgtagggc
     9781 agaatgctgg gcaatatttt gcgtcaccag gccgaggtga atcagctggc ggatcacgct
     9841 cacccaatgt tcatggcttt tatcacggcc catgccatag actttcagtt tgtcatgacc
     9901 atagtcgcgg atacgctggt tattagcacc gcgaatcact tccaccacat aacccatccc
     9961 aaaccgctga ttcacacgac caatggtgga aagggcaatc tgcgcatcgg ttgaaccatc
    10021 gtactgtttt ggcggatcga ggcagatatc gcagttcccg cacggctcct gacgcccttc
    10081 gccaaaatag ttcagcaata ccaaacgacg gcaagtttgc gcttcagcaa acgcgcccat
    10141 cgcattgagt ttatgacgtt cgatatcctg caactgcccc tgcggcttct cttccagaca
    10201 acggcgcagc cacgccatat cagccggatc gtaaaacagc atcgcttccg cgggcaggcc
    10261 atcacgcccg gcgcgtccag tttcctgata ataggattcg atattgcgcg gaatatcaaa
    10321 atgcaccacg aagcgaacgt taggcttgtt gatgcccatg ccgaacgcca ccgtcgccac
    10381 cacaatttgc aggtcatcgc gctggaattt ttcctgcaca tcagcgcgaa cattattttc
    10441 cagcccggca tgataggccg ccgcgctaat tcccttgctt tgcaggcgcg cagcggtgtc
    10501 ttctactttc gcacgactat tacagtaaat aatccccgac ttaccgcgtt gttcctgtac
    10561 ataacgcatc aactgatcga gcggtttgaa tttctccatc agcatgtagc gaatattcgg
    10621 acggtcaaaa ctgctgattt gaatcagcgg atcgttcagc cccagcaggc gcacgatatc
    10681 ctggcgcgtg gtgtcatcgg ctgtggcagt cagcgccata aacgggagcg tcggaaaacg
    10741 ctgacgcagc tgaccgaggg cagcgtattc cgggcggaaa tcgtgacccc attgggagat
    10801 acagtgcgct tcatcaacgg ctaataacac cggattccag tgcgccagat gctcaagaaa
    10861 gttatccagc atcaggcgtt ccggcgcgat ataaagcagg ctgatttgcc cggtgcgaca
    10921 gcctgtcatc acttcaagct gctgctcgcg ggtttgcgtc gagttaaggc acgccgccgc
    10981 cacgccgttg gcttgcagtt gatctacctg atctttcatc aacgaaatca gcggtgagac
    11041 aaccacggta aggccgttta gcaataaggc agggatttga tagcaaaggg attttccgcc
    11101 accagtgggc atgacgacca ggcaatcgcg accggaaagt acggtgtcga taatctcttc
    11161 ctggccgggg cgaaactgtt ggtagccaaa ggtttcttgt aaaacctgtt tagctccgga
    11221 ctccagattc aacacttccg cctgcgccac attcaccccg attgcctgaa ataaaaacag
    11281 gcgctatttt cagcgcctgc gcgagaaact gcaatgcttt tagtctctga gagggtaatc
    11341 ctggatgctg gattccagat ttgccaacca ttcatcaaaa caggtcgttt agcataactc
    11401 ccacaccaac gcgggtctgg ttgaagttat agtcgatgag cgattcgccg taaccactgt
    11461 aaacctgagt ataaaggcgc acatgtttgg tgatcgggta gcttaagcct aactccgcgc
    11521 cgccgtaacc ggtgttccag ttgtactgcc ctttcgcgct cagtaccgca tcaccgaggt
    11581 gatagccgat tttaagctgg tagtaaccca tatatttggt gatgtccggg ttatcgtccg
    11641 tattccccac cacataccac ggcttcacct ctaccagcca gttaccgttt tctgccatca
    11701 ggcgggtata aaggcggttc cagctgcggg aagtcgggtc tgaacgcccg tttgagtcgt
    11761 ggttgtagcc catttccaca tcgcgcagcg tccagccagc aaagttgtaa tccgtggcaa
    11821 aaccgaggaa caattgcggt tcgtagttag tttcacgaaa cggtgatgac tcatcgctat
    11881 tagaaagctg ccaccaggat ttctgtgtgt aggaggcgcc cagtacagag tttggcccca
    11941 aaatcccacg ccacagcgga aacgccaggc tcaactggaa ctttacttca tccttacgcg
    12001 cgttttccgc ccagtcgtaa ctggcaatcg cttctttatt cagatcgctg gtttgggtgt
    12061 agatgatgta gttggtgtca taaggataaa gggtgaacgg attgtcatgc tcctgcagca
    12121 tattggcgat aatactgcca cgcaccgctg gcgcgtcatg cacctctttc accgttgcct
    12181 cttgtgcata tactgccata ggcaacataa acaccggcaa caaccagccc tgcagagtcc
    12241 gcatcgctcg tgttctccgt aacgaattat tgataaggtg aatatttttc tgcgggctat
    12301 tttacatact taactccata gccgttagtt atcccttcct aaagtagaaa ccaacaataa
    12361 ataagcataa aatcaacatt tcattaacta atgaagtgta aggatgttat gtctgccgta
    12421 ctgaccgctg aacaagccct caaattagtg ggtgagatgt ttgtttatca catgccattt
    12481 aaccgcgcat tggggatgga actggagcgt tacgaaaaag agttcgcaca gctggccttt
    12541 aaaaataagc cgatgatggt gggcaactgg gcgcaaagca ttttacacgg cggggtcatt
    12601 gcgtcggcgc tggatgtcgc cgcaggtctg gtgtgcgtgg gaagtacctt aacccgccac
    12661 gaaaccatca gtgaagatga actacgccag cggctatcgc ggatggggac cattgatctt
    12721 cgcgtcgatt atctgcgccc aggcagaggc gagcgtttta ctgctaccag tagcctgtta
    12781 cgtgcaggca ataaagtcgc cgtcgcccgc gttgaattac acaatgaaga acagctttat
    12841 attgccagtg ccaccgccac ctatatggta ggttgaagac ataaaagcag gtaaactgct
    12901 gttaccaatt aaaaatgagt attcccgatg gatgcaaaac aaacgcggca gggcgtatta
    12961 ctcgctcttg ccgcttattt tatttggggt attgcgccag cgtacttcaa actgatttac
    13021 tacgtgcccg ccgatgagat cctgacgcat cgcgtgatct ggtcgttttt ctttatggtg
    13081 gtgctgatga gcatttgccg ccagtggtcc tatttaaaaa cgctgattca gacgccacag
    13141 aaaattttta tgctggcagt ctctgccgtg ctgattggcg gcaactggct actgtttatc
    13201 tgggcggtga acaatcacca tatgctggaa gcgagccttg gttactttat taacccgctg
    13261 gtgaacattg tgctggggat gattttcctc ggcgagcgat tccgccggat gcaatggctg
    13321 gcggtgattc tggcgatatg tggagtgctg gttcagttgt ggacttttgg ttcactgccg
    13381 atcatcgcgc tggggctggc gtttagtttt gctttctacg gcctggtgcg caagaagatt
    13441 gctgttgaag cgcaaaccgg catgttaatc gaaactatgt ggttgctgcc cgtggcggcg
    13501 atttacctgt ttgctattgc cgacagctca accagtcata tggggcaaaa cccgatgtcg
    13561 ctgaatttac tgctgatcgc cgccggtatt gtcaccaccg tgccactgtt gtgttttact
    13621 gcagctgcga cacgcctgcg tctctcaacg ttaggctttt tccagtacat tggtccgacg
    13681 ctgatgttcc tgctggctgt gacgttttac ggtgaaaaac cgggtgccga taagatggta
    13741 actttcgcct ttatttgggt ggcgctggca atttttgtga tggatgc
//
LOCUS       DAZFRG010000123        13652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFRG010000123 DAZFRG010000000
VERSION     DAZFRG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.123"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..1184
                     /gene="citC"
                     /locus_tag="ACHKUC_002941"
     CDS             126..1184
                     /gene="citC"
                     /locus_tag="ACHKUC_002941"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO6619165.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1199..1495
                     /gene="citD"
                     /locus_tag="ACHKUC_002942"
     CDS             1199..1495
                     /gene="citD"
                     /locus_tag="ACHKUC_002942"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO6619166.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1492..2400
                     /gene="citE"
                     /locus_tag="ACHKUC_002943"
     CDS             1492..2400
                     /gene="citE"
                     /locus_tag="ACHKUC_002943"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO6619167.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2411..3943
                     /gene="citF"
                     /locus_tag="ACHKUC_002944"
     CDS             2411..3943
                     /gene="citF"
                     /locus_tag="ACHKUC_002944"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO6619168.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3947..4498
                     /gene="citX"
                     /locus_tag="ACHKUC_002945"
     CDS             3947..4498
                     /gene="citX"
                     /locus_tag="ACHKUC_002945"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO6619169.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLISFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4473..5351
                     /gene="citG"
                     /locus_tag="ACHKUC_002946"
     CDS             4473..5351
                     /gene="citG"
                     /locus_tag="ACHKUC_002946"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO6619170.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNISP
                     GGSADLLILTWFLAQI"
     gene            5402..6865
                     /gene="citT"
                     /locus_tag="ACHKUC_002947"
     CDS             5402..6865
                     /gene="citT"
                     /locus_tag="ACHKUC_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO6619171.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6979..7785
                     /gene="rna"
                     /locus_tag="ACHKUC_002948"
     CDS             6979..7785
                     /gene="rna"
                     /locus_tag="ACHKUC_002948"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO6619172.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYDDFDRYVLALSWQ
                     TGFCQSQYDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8015..8425
                     /gene="rnk"
                     /locus_tag="ACHKUC_002949"
     CDS             8015..8425
                     /gene="rnk"
                     /locus_tag="ACHKUC_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO6619173.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8568..8657
                     /gene="yldA"
                     /locus_tag="ACHKUC_002950"
     CDS             8568..8657
                     /gene="yldA"
                     /locus_tag="ACHKUC_002950"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO6619174.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8781..9209
                     /gene="uspG"
                     /locus_tag="ACHKUC_002951"
     CDS             8781..9209
                     /gene="uspG"
                     /locus_tag="ACHKUC_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO6619175.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9330..10895)
                     /gene="ahpF"
                     /locus_tag="ACHKUC_002952"
     CDS             complement(9330..10895)
                     /gene="ahpF"
                     /locus_tag="ACHKUC_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO6619176.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVHGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11066..11629)
                     /gene="ahpC"
                     /locus_tag="ACHKUC_002953"
     CDS             complement(11066..11629)
                     /gene="ahpC"
                     /locus_tag="ACHKUC_002953"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO6619177.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12001..12747
                     /gene="dsbG"
                     /locus_tag="ACHKUC_002954"
     CDS             12001..12747
                     /gene="dsbG"
                     /locus_tag="ACHKUC_002954"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO6619178.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
     gene            12952..>13652
                     /locus_tag="ACHKUC_002955"
     CDS             12952..>13652
                     /locus_tag="ACHKUC_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6619179.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLMT"
BASE COUNT         3310 a         3543 c         3388 g         3411 t
ORIGIN      
        1 gagaaaaaga tatacctcga tcgccccctt tctcccaagt gaaaataaaa ggttatcaat
       61 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
      121 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
      181 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
      241 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta
      301 ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg actggcgctg acattagcca
      361 ctgaactgat aaacctcgcc tatgagcggc atagcacgca tctgtttatt tataccaaaa
      421 ccgaatacga ggcgctgttc cgccagtgcg gtttttccac gctgaccagc gtacccggcg
      481 tgatggtgct gatggaaaac agcgccacgc gactgaaacg ctatgccgaa tcgctgaaaa
      541 aatttcgtca tccagggaac aagattggct gcattgtgat gaacgccaat ccctttacga
      601 atggtcaccg ttatctgatt caacaggctg cggcacagtg cgactggttg catctgtttt
      661 tagtcaaaga agattcttca cgcttcccct atgaagaccg gctggatctg gtgttaaaag
      721 gcaccgccga tattccacgc ctgactgtgc atcgcggctc cgaatacatc atctcccgcg
      781 ctacgttccc ttgctacttc attaaagaac agagcgtcat taaccattgt tacaccgaaa
      841 ttgatctgaa gattttccgt cagtacctcg ctcccgcgct gggtgtaact caccgctttg
      901 tcggtactga acccttttgt cgcgttactg cccagtacaa ccaggatatg cgctactggc
      961 tggaaacacc gactatctcc gcaccgccca tcgaactggt tgaaattgag cggctgcgtt
     1021 accaggagat gccgatatcc gcttcccggg tacgtcaact gctggcaaaa aacgatctca
     1081 cggctatcgc gccgctggtc cctgcagtca cgctgcatta tttgcagaac ctgcttgagc
     1141 actcccgcca ggacgcggca gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat
     1201 gaaaataaac cagcccgccg ttgcaggcac ccttgagtct ggggatgtga tgatacgcat
     1261 cgccccactc gatacgcagg atatcgacct gcaaatcaac agcagcgttg agaaacagtt
     1321 tggcgatgca attcgcacca ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt
     1381 acagctgaat gtcgatgaca aaggcgcact ggactgcatt ttacgtgcac gactggaagc
     1441 cctgctggca cgcgccagcg gtatcccggc tctgccatgg gaggattgcc aatgatttcc
     1501 gcttcgctgc aacaacgtaa aactcgcacc cgccgcagta tgttgtttgt gcctggtgct
     1561 aatgccgcga tggtcagcaa ctccttcatc tacccggctg atgccctgat gtttgacctc
     1621 gaagactccg ttgcattgcg tgaaaaagac accgcccgcc gcatggttta ccacgcgctg
     1681 caacatccgc tttatcgcga tattgaaacc attgtgcgtg taaacgcgct ggattccgaa
     1741 tggggtgtta acgacctgga agccgtcgtt cgcggtggtg cggacgttgt gcgtctgccg
     1801 aaaaccgata ccgctcagga tgttctggat atcgaaaaag agatcctgcg tatcgaaaaa
     1861 gcctgtggtc gtgaacccgg cagtaccggc ctgctggcgg cgattgaatc tccgctgggc
     1921 attacccgcg cggtagaaat cgctcacgct tccgagcgtt tgatcggtat cgccctcggt
     1981 gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc cggaaggaac tgaactgctg
     2041 ttcgctcgct gctccatttt gcaggccgcg cgttctgcgg gtattcaggc gttcgatacc
     2101 gtctattccg acgctaacaa cgaagccgga ttcctgcaag aagccgccca catcaaacag
     2161 ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga ttgatctgct gcacaacctc
     2221 tacgcaccga cccagaaaga agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc
     2281 gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca agatggtgga cggtccggtt
     2341 atcgatcgcg cccgtctggt gctctcccgt gcagaacttt ccggcatccg cgaagaataa
     2401 ggcaatcaaa atgacgcaga aaattgaaca atctcaacga caagaacggg tagcggcctg
     2461 gaatcgtcgc gctgaatgcg atcttgccgc tttccagaac tcgccaaagc aaacctacca
     2521 ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg gaagaagcga ttcgtcgctc
     2581 tggtttacag gacggcatga cggtttcctt ccatcacgct ttccgtggcg gtgacctgac
     2641 cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt aaaaacctga ctctggcctc
     2701 cagctccctg agtgattgcc atgcgccgct ggtagaacac attcgccagg gcgtggttac
     2761 ccgcatttat acctctggcc tgcgcggtcc actggcggaa gaaatctccc gtggtctgct
     2821 ggcggaaccg gtgcaaatcc actctcacgg cggacgtgtg catctggtac agagcggcga
     2881 actgaatatc gatgtggctt tcctcggcgt cccgtcctgt gatgaattcg gtaatgccaa
     2941 cggctactcc ggtaaagcct gctgcggctc cctcggctat gcaatggttg atgccgacaa
     3001 cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct tatccgcata atccggcaag
     3061 cattgagcaa gatcaggttg atctgatcgt caaagttgat cgcgttggcg atgctgcaaa
     3121 aatcggcgct ggtgctaccc gtatgaccac taacccgcgc gaactgctta ttgcccgtag
     3181 cgctgcggat gtgattgtta actctggcta cttcaaagaa ggtttctcca tgcagaccgg
     3241 caccggcggc gcttcgctgg cggtaacccg tttcctggaa gacaaaatgc gtagccgcga
     3301 tattcgcgcc gacttcgccc tcggcggtat taccgcaacg atggttgatc tgcacgaaaa
     3361 aggtttgatc cgcaaactgc tggatgtgca gagctttgac agccatgctg cgcaatcgct
     3421 ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag tacgctaact gggggtcgaa
     3481 aggcgcagcg gttgatcgtc tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca
     3541 gttcaacgtt aacgtgctga ccggctctga cggcgtactg cgtggtgctt ccggtggtca
     3601 ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc gcgccgctgg tacgcggtcg
     3661 tattccgacg ctggtggata acgtgctgac ctgcatcacc ccgggctcca gtgtcgatat
     3721 tctggtcaca gaccacggca tcgctgttaa cccggcacgt ccggagctgg cagaacgtct
     3781 gcaggaagcg gggatcaaag tggtttctat tgagtggctg cgtgaacgtg cgcgtctgct
     3841 gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg
     3901 cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa taagccatgc acctgcttcc
     3961 tgaactcgcc agccaccatg cggtgtcaat tcccgagctg ctcgtcagcc gggatgaaag
     4021 gcaagcacgg caacacgcct ggctcaagcg ccatcctgtt ccactgatct cctttaccgt
     4081 ggttgcgcct gggccgatta aagacagcga ggtcacacgc cgaattttta atcatggcgt
     4141 gacagccttg cgtgccttag ccgcaaaaca gggctggcaa attcaggagc aggctgctct
     4201 ggtttccgcc agcgggccgg agggcatgtt gagcattgcc gccccggctc gcgacctcaa
     4261 gctcgccacc attgagcttg aacatagtca tcctctcggg cggttatggg atatcgatgt
     4321 cctgacgccc gaaggcgaca ttctctcccg ccgcgattat tcactgccgc ctcgccgctg
     4381 cctgttgtgc gagcaaagcg cagctgtttg cgcgcgtgga aaaacccatc aactgaccga
     4441 tttactcaac cgcatggagg cgctgctaaa cgatgtcgat gcctgcaacg tcaactaaaa
     4501 ccacaaagct tgcgacgtca ttaatcgatg agtacgccct gctgggctgg cgcgccatgc
     4561 tgactgaagt caacctgtca ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc
     4621 acaaagatat ggcgctggaa gatttccacc gcagcgcgct ggcgattcag ggctggttac
     4681 cccgtttcat tgaatttggt gcctgtagtg cagaaatggc accggaagcg gtactcaacg
     4741 gattacgccc aatcggtatg gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa
     4801 acacgcataa aggaagcatt ttttctttag ggctactgtg tgcggcaatt ggccgtttgc
     4861 ttcagctcaa ccagccggta acgccaacaa ccgtttgttc tacggcggca agtttctgcc
     4921 gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg actgacggca ggtcaacggt
     4981 tgtaccagca gcttggcctt accggcgcac gcggtgaagc cgaagcgggt tatccactgg
     5041 tgatcaacca cgccttgccg cattacctca ctctgctgga tcaggggtta gatcctgagc
     5101 tggcattgct cgataccttg ctcctactga tggctaccaa cggcgatacc aacgttgcat
     5161 cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga ggcgcaaaca ttattaaaca
     5221 atgggggcat ccgaaccccc gccgatctcg attatctccg gcagttcgac agggagtgta
     5281 tcgaacgaaa tatcagtcca ggcggcagtg ccgacctgct gattcttacc tggtttttag
     5341 cacagattta attatttaag cacttgataa atttggaaat attaattttc ggagaacccg
     5401 tatgtcttta gcaaaagata atatatggaa attattggcg cccctggtgg tgatgggtgt
     5461 catgtttctt atccctgtac ctgacggtat gccgccgcag gcgtggcatt actttgcagt
     5521 gtttgtggca atgattgtcg gcatgatcct cgagccaatt ccggcaacgg cgatcagttt
     5581 tattgcggtt actatttgcg ttattggcag taattacctg ctctttgatg ccaaagaatt
     5641 agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc
     5701 cagcaccacg gtatggctgg tatttggcgc atttattttt gcattagggt atgaagtttc
     5761 cgggttaggc cgtcgtattg cccttttcct ggtgaaattc atgggcaaac gcacgctgac
     5821 gttgggttat gcgattgtca ttatcgacat tctgctggca ccgtttacac cgtccaacac
     5881 cgcgcgtacc gggggtacgg tttttccggt cattaaaaac ctgccgccgc tgtttaaatc
     5941 attcccgaac gatccgtccg cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat
     6001 tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct
     6061 ggagttcgtc agcaaaattg ccggtatcca gattagctgg ttgcagtggt tcctctgctt
     6121 cctgccggtt ggggttatct tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa
     6181 accggaaatc acacacagtg aagaagtggc aacctgggcg ggtgatgaac taaaaaccat
     6241 gggtgcgctg acacgcagag agtggacgct gattggcctt gtattgctca gcttaggttt
     6301 gtgggtattt ggcagtgaag tcattaatgc tactgcggta ggtctgctgg cagtttcgct
     6361 gatgctggcg ctgcacgttg tgccgtggaa agacattact cgctataaca gcgcatggaa
     6421 cacgctggtc aacctggcaa cgctggttgt tatggcgaat ggtctgaccc gttctggctt
     6481 tatcgactgg ttcgccaaca cgatgagtac gcacctggaa ggcttctcac caaacgcaac
     6541 ggtgattgta ctggttctgg tgttctactt tgcccactac ctgtttgcca gcctgtctgc
     6601 gcacaccgca accatgctgc cggttattct ggccgtcggt aaagggattc cgggcgtacc
     6661 aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt atcatgggct gtctgacgcc
     6721 ttatgcaacc ggtcctgggg tgattattta cggctgtggc tatgtgaaat caaaagatta
     6781 ctggcgtctt ggcgcaatct tcggggtgat ttacatctct atgctgctgt tggttggctg
     6841 gccgattctc gccatgtgga actaatacgt tcgccaaacg ctgaataaat catcacgccc
     6901 gccaggtaag cccacctggc gggctttttt atgatttaat agataatatt cagtgtatgt
     6961 atgagttcca cacccattat gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct
     7021 ctgcttccct tctcttctgc caacgccgtg gcgttgcagg caaaacagta tgacgatttt
     7081 gatcgctatg tcctggccct ctcatggcaa accggatttt gtcagagtca atacgaccga
     7141 aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt
     7201 ttgaccgtac atggtctgtg gccaggattg cctaaatcgg ttgctgcccg tggtgttgat
     7261 gaacgccgct ggatgcgctt cggttgcgct actcgcccaa tcccgaatct gccagaagca
     7321 cgtgccagcc ggatgtgttc atcgccggaa accgggttat cactggaaac ggcagctaaa
     7381 ctaagtgaag tcatgccagg agctggcgga cgttcctgcc tggaacgcta cgaatatgcc
     7441 aaacacggtg cctgctttgg ttttgatcca gacgcatact tcggcacgat ggtacgcctg
     7501 aatcaagaaa ttaaagagag cgaagccgga aaattccttg cggataatta cggtaaaaca
     7561 gtgagccgcc gtgactttga cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa
     7621 gcagttaagc taacgtgcca gggtaaccct gcgtatttga ctgaaattca gatctcgatc
     7681 aaagctgacg ccatcaacgc tccgctttct gcaaactcat tcttgccaca accacaccca
     7741 ggtaactgtg gcaaaacctt tgtgattgat aaagcaggtt attaatcctc tgatttacaa
     7801 aacacgctaa ttcgtctccc ataaaccatg acgattaaga tttatacata atgcgctaca
     7861 ttgaggaatt ccctgaatgt gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca
     7921 tcatgtgagt taaaccctcg ccgcctgacg gtgagggttt tcttttgggt atgtttatct
     7981 gcgacactcg cagtaccgac gacatggagt aaaaatgtcc agaccaacta tcatcattaa
     8041 cgacctggat gccgaacgca tcgatattct gctggagcaa cccgcctatg ctggtttgcc
     8101 aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa atgtgttcgc cagaagagat
     8161 gccacacgac gtggtgacaa tgaacagccg ggttaaattc cgcaatctta gcgatggcga
     8221 agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc gacagcaata ctcagctttc
     8281 cgttatggct ccggtaggtg ccgcactgct ggggctgcgc gttggcgatt ccattcactg
     8341 ggaacttccg ggcggcgttg caacccacct tgaagtgctg gaactcgaat accagccaga
     8401 agctgctggc gactacctgc tttaatccca tctgcacaag cgataactcg ctttgcccag
     8461 aggatgcact cgcatcctct ttccgctcaa atatcaacca gatttatcat tttgcgctta
     8521 agcgcagcat tctaatctga actggttatt catctgcgga acctcatatg agcgaagctc
     8581 tcggtatcac tgcattttat atattcatag cagcaattat tgttgctgca gtgctatacc
     8641 ttgaacaacg ctggtagcgc ctggttttat ttattttcca gctcaatagc aaattcagat
     8701 caaatcagcc tcgcaaatta tagattcata accaataatg tgctgttatg tatacatgca
     8761 aaaacaagga gagaatgctt atgtacaaaa caatcatcat gccagtggat gttttcgaaa
     8821 tggaattgag cgacaaagcg attcgccacg ctgaattcct cgcccaggat gacggagtta
     8881 ttcatctact tcacgtacta ccggggtcag ccagcctgag cctgcaccgt tttgccgctg
     8941 atgtacgtcg ttttgaagag catctgcaac atgaagcaga agaacgtttg caaacgatgg
     9001 tcagccactt caccatcgat ccttcccgca ttaagcaaca tgtccgtttt ggtagtgtgc
     9061 gggatgaagt taacgagtta gcgaaagaac ttgatgcaga tgtagtagtg attggttcgc
     9121 gtaacccatc aatttcaact catctgttgg gttctaacgc ctcgagcgta atccgccacg
     9181 ccaatttgcc ggtgctggtt gtgcgttaat tgccagaata agtctcccgc cctgcccgga
     9241 gccatccctg cagggctttt taatgcaata aaaaagccgc caggtttgac cccggcggct
     9301 aagcaattac aggtgaatct tacttcttct tatgcagttt tggtgcgaat caggtagtca
     9361 aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg cttgtacgga
     9421 accgtcgtac agtcacccgc tgcgaacacg cctttgacgt tggtttcgca tttcgcatca
     9481 atgataatct cgcccatgcg attacgttcg actgcgcctt ccagccagtt ggtgttcggc
     9541 agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca
     9601 cgatcgcgat attccagacc tacgactttg ctaccgtcgc ctttcacttc cgtggtttgc
     9661 gcattcagaa taatgtcgac gtttttcagg ctgcgtagtt tgtcctgcag aacctggtcg
     9721 gctttcattt ctggcgcaaa ttccagcagc gttacgtgct caacgatacc cgccaggtca
     9781 attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt gcctttaaac
     9841 agcgggccgt cgcagtgcgg gcaataggtc acgcctttgg tgcgatactg atcttcgcca
     9901 tgaacgttca tgttgcgcca ttttgcacca gtcgccacga taatgctgcg tgctttcagt
     9961 accgcgccag aagctgtttc aatctgatgc aggccgcctt caaccgctgc cgggatcagt
    10021 ttgctcgcgc tctggctgtc gatcacatca acgtcgtatt catcaacatg aactttcagc
    10081 gcacctgcca gtttctggcc ttcggtcttc ggtacagaaa tgtagttttc gatatcaacg
    10141 gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt
    10201 gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac caacgattaa tacgtcataa
    10261 gcatcacgct tattcagctc ttctgccgca cgtttttccg caccagtatc aattttggca
    10321 acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc
    10381 ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca
    10441 gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac cacgtccggg
    10501 cagttgtggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga
    10561 atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca caacaacgcc
    10621 agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg tccctggttg
    10681 gaacctgggt tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta
    10741 aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat
    10801 ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa
    10861 gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc
    10921 atcatgcaag cggcctctgc ggctagctgg atgcagcttg catcatggtg cggggaagaa
    10981 aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatctc gcgccggagt
    11041 tagtagcgcg aaagacaaag gaaatttaga ttttaccaac caggtccaga gacggagcca
    11101 gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt
    11161 actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg
    11221 taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca
    11281 gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga
    11341 tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga
    11401 agtgggtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtaat
    11461 ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga
    11521 agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt
    11581 tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct
    11641 ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca
    11701 ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac
    11761 cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta
    11821 ccctttggta tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa
    11881 cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct
    11941 atgacagtga taggttatgc cttttactcg acttttgcac tgactgaaaa ggacaaatta
    12001 atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt
    12061 cctgccccag taaaagcgat tgaaaaacag ggtattacaa tcatcaaaac gttcgatgcc
    12121 cctggaggaa tgaaaggtta tctcggtaag tatcaggata tgggcgtcac catctacctg
    12181 actccagatg gtaagcacgc tatctctggt tacatgtaca acgaaaaagg tgaaaacctg
    12241 agtaacacgc ttatcgaaaa agaaatttac gcacccgccg gacgtgaaat gtggcaacgg
    12301 atggaacaat cccactggct cctcgacggt aaaaaagacg cgccggtcat tgtctacgtc
    12361 ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggctcg tccgtgggta
    12421 gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaaacc agaaagcccg
    12481 gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca acaatatgaa
    12541 gcctctggtg gcaagcttaa gctaagcgtg cctgcaaacg taagtacaga gcaaatgaaa
    12601 gtgttaagcg acaacgagaa actcatggac gatctggggg caaatgtcac accagctatc
    12661 tattacatga gtaaggaaaa taccctgcaa caagccgtcg gattacccga tcagaaaacg
    12721 cttaatatca ttatgaggaa taaataaagg ataattcatg gatggttgaa atatatcctt
    12781 gctttttaac catcctaaaa acgcatgaat tgctcgtaac ccacctgaca gctatgctat
    12841 tttagaaaat cttaatccaa agttaagcag aaaaacataa cacatataaa tacaacaaca
    12901 ttattgatca agtgaatgat ttatggaaga gacaatacaa cggagtatgt tatggctaat
    12961 ctttatgatc taaaaaaatt tgacctcaat ttactggtca tatttgaatg tatttatcaa
    13021 catctcagca ttagtaaagc ggcagaatca ttatatataa cgccctctgc tgttagtcag
    13081 tcgttacaac gtttacgtgc gcaatttaat gacccgttat tcattcgctc agggaaaggt
    13141 atcgcgccga ctacaacagg gttaaacctg catcaccatc ttgagaaaaa tctcagaggc
    13201 cttgaacaaa cgatcaatat cgttaataag tctgaactaa agaaaaattt tattatctac
    13261 ggtcctcagc ttatttcttg tactaataac aacatgctaa tccgctgtct tagacaagat
    13321 acttcaatcg atatagaatg ccatgacatc ctgctttcag ctgaaaatgc cgaagaactg
    13381 ttggttcaac gcaaaactga cctggcaatt acgctgcaac cagtaattag tcgctctgtc
    13441 atctgcatgc cgttacacac tattcgcaat tcccttatat gtagcaacaa acatcctcgt
    13501 attactgata ccagtaacta tgaacaaatt atcgccgaag aattcaccct gttgatatct
    13561 aaatcagcag ggattgacga aattcaaatg gacatagatg aacgttttat gaaccgtaaa
    13621 gtttcatttc gcagttcttc tttgatgact at
//
LOCUS       DAZFRG010000124        13631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFRG010000124 DAZFRG010000000
VERSION     DAZFRG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.124"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1332
                     /gene="chbC"
                     /locus_tag="ACHKUC_002956"
     CDS             <1..1332
                     /gene="chbC"
                     /locus_tag="ACHKUC_002956"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO6619180.1"
                     /translation="KVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFL
                     SFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERK
                     VDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVHR
                     NWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLA
                     SLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALA
                     AGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINE
                     PILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFN
                     TNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1383..1733
                     /gene="chbA"
                     /locus_tag="ACHKUC_002957"
     CDS             1383..1733
                     /gene="chbA"
                     /locus_tag="ACHKUC_002957"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO6619181.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1741..2583
                     /gene="chbR"
                     /locus_tag="ACHKUC_002958"
     CDS             1741..2583
                     /gene="chbR"
                     /locus_tag="ACHKUC_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO6619182.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2687..4039
                     /gene="celF"
                     /locus_tag="ACHKUC_002959"
                     /gene_synonym="chbF"
     CDS             2687..4039
                     /gene="celF"
                     /locus_tag="ACHKUC_002959"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6619183.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4052..4810
                     /gene="chbG"
                     /locus_tag="ACHKUC_002960"
     CDS             4052..4810
                     /gene="chbG"
                     /locus_tag="ACHKUC_002960"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO6619184.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFVAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(4857..7118)
                     /gene="katE"
                     /locus_tag="ACHKUC_002961"
     CDS             complement(4857..7118)
                     /gene="katE"
                     /locus_tag="ACHKUC_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO6619185.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEGEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCSYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVSADDGTVLSIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADCASGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            7301..7564
                     /gene="cedA"
                     /locus_tag="ACHKUC_002962"
     CDS             7301..7564
                     /gene="cedA"
                     /locus_tag="ACHKUC_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO6619186.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7611..9002)
                     /gene="tcyP"
                     /locus_tag="ACHKUC_002963"
     CDS             complement(7611..9002)
                     /gene="tcyP"
                     /locus_tag="ACHKUC_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO6619187.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANSTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESITSFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9135..9725)
                     /gene="ydjM"
                     /locus_tag="ACHKUC_002964"
     CDS             complement(9135..9725)
                     /gene="ydjM"
                     /locus_tag="ACHKUC_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6619188.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9889..10557)
                     /gene="hxpB"
                     /locus_tag="ACHKUC_002965"
     CDS             complement(9889..10557)
                     /gene="hxpB"
                     /locus_tag="ACHKUC_002965"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO6619189.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLIEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10704..11240
                     /gene="yniB"
                     /locus_tag="ACHKUC_002966"
     CDS             10704..11240
                     /gene="yniB"
                     /locus_tag="ACHKUC_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO6619190.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11281..12141)
                     /gene="yniA"
                     /locus_tag="ACHKUC_002967"
     CDS             complement(11281..12141)
                     /gene="yniA"
                     /locus_tag="ACHKUC_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO6619191.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12247..12537)
                     /gene="ghoS"
                     /locus_tag="ACHKUC_002968"
     CDS             complement(12247..12537)
                     /gene="ghoS"
                     /locus_tag="ACHKUC_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6619192.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            complement(12638..13567)
                     /gene="pfkB"
                     /locus_tag="ACHKUC_002969"
     CDS             complement(12638..13567)
                     /gene="pfkB"
                     /locus_tag="ACHKUC_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO6619193.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLAHENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3438 a         3214 c         3477 g         3502 t
ORIGIN      
        1 aaggtactcc tcccttttgc agttaaaata ggaaagcagc cacacgttaa tgcaatcaaa
       61 aatggcttta ttcgcttaat gccgttaacc cttgcggggg ccatgtttgt attaattaac
      121 aacgtttttc taagctttgg ggaggggtcg tttttttatt ccttaggtat tcgcctggat
      181 gcctcaacca ttgaaacact taatggtctg aaaggtattg gcggcaacgt atataacgga
      241 acattaggga taatgtcttt aatggccccg ttctttattg gcatggcgct ggcagaagag
      301 cgtaaagtcg atgcgctggc ggccgggttg ttatccgttg cagcatttat gaccgtcacc
      361 ccatatagtg tcggtgaggc ctatgcggtt ggcgcaaact ggttaggtgg ggcgaatatc
      421 atctccggga ttattattgg cctggtggtg gcagaaatgt ttacctttat tgttcatcgt
      481 aactgggtca ttaagttacc tgatagcgtt ccgacatcgg tttcgcgctc tttctcagca
      541 ttaattcccg gttttattat tctttccgtg atggggatta ttgcctgggc gttgaatacc
      601 tggggcacca acttccatca gatcattatg gataccatct caaccccact ggcatcgttg
      661 ggtagcgtgg taggctgggc ctatgtgatc tttgttccac tgctctggtt cttcggtatt
      721 catggcgcgc tggcgctgac cgcactggac aacggcatta tgacgccgtg ggcgctggaa
      781 aatatcgcga cctatcagca atatggttcc gtcgaagcgg cgctggcagc cggtaagacc
      841 ttccatatct gggccaagcc gatgctggac tcctttattt tccttggtgg cagtggtgcg
      901 actttaggcc tgatcctggc tatctttatc gcttctcgcc gtgctgatta tcgtcaggtg
      961 gcaaaactgg cgctgccgtc cggcatcttc cagattaacg aaccgattct gtttggtctg
     1021 ccaattatca tgaacccggt gatgtttatc ccgtttgtac tggtacaacc gattctggcg
     1081 gcaatcaccc tggcagcgta ctacatgggc attattccac cggtgaccaa tattgcaccg
     1141 tggaccatgc ccaccggtct gggagccttc tttaacacca acggtagcgt cgccgcattg
     1201 ctggtcgcac tcttcaacct tggtatcgca acgttaattt atctgccctt tgttgtggtc
     1261 gctaacaaag cacaaaacgc gattgataaa gaagagagcg aagaagatat cgctaacgcc
     1321 ctgaaatttt aatcgatgcc ggtacgggta atcgtgccgg taagaataga gaggaacgat
     1381 gtatgatgga tctcgataat attcccgata cgcaaacgga agctgaagag ctggaagaag
     1441 tggtgatggg gcttatcatc aactccggac aagcgcgtag cctggcgtat gcggcactga
     1501 aacaggcgaa gcagggcgat tttgccgcag caaaagcgat gatggatcag tcacggatgg
     1561 cattgaatga agcgcatctg gtacagacga aactgattga aggcgatgct ggcgaaggta
     1621 agatgaaagt gagtctggtg ctagtccacg ctcaggatca tttaatgacg tccatgcttg
     1681 cgcgtgaact gattactgaa ttaattgagc ttcatgaaaa actgaaggca taaggagtcg
     1741 atgatgcagc cagtgattaa cgcgccggaa attgccactg cccgagaaca gcagttgttt
     1801 aatggcaaaa acttccatgt gtttatctac aataaaactg agagtatcag cggactgcat
     1861 cagcacgact attatgaatt tactctggta ttaaccgggc gttatttcca ggagattaac
     1921 ggtaagcgcg tgttactgga acggggcgat tttgttttta ttccgttagg ttcgcaccat
     1981 caaagttttt atgaatttgg tgccacgcgt atattgaacg ttgggatcag taaacgcttt
     2041 tttgagcagc attacctgcc gttgttgcct tattgctttg tagcttcgca ggtataccgg
     2101 accaataacg cttttctcac ctatgtggaa acagtgattt cttcattgaa tttccgcgaa
     2161 acagggcttg aagagtttgt tgagatggtt actttttatg tcattaaccg tttacgtcat
     2221 taccgcgaag aacaggtgat tgatgatata ccgcagtggc tgaaaagtac ggtagaaaag
     2281 atgcatgata aagagcagtt tagtgaatcg gcgctggaga atatggtgac gttgtcagcc
     2341 aaatcacagg aatatttgac gcgagcgact caacgatact atggtaaaac gccaatgcag
     2401 attattaatg aaatccgtat taattttgcc aaaaaacaac tggaaatgac caactattca
     2461 gtgacggata ttgcgtttga ggccggttat agtagcccga gcttgtttat taaaacgttt
     2521 aagaaattaa catcctttac gcctaagagc tatcgtaaga aattgactga atttaatcag
     2581 taagtcgtta tacctgacaa ttcacatatc tgtcctgttg ctggattatt tatgtccggg
     2641 gggcagatat gccagatatc agtattctgt actgaaggga gaaattatga gccagaaatt
     2701 aaaagtcgtc actattggtg gcgggagcag ctataccccg gagttactgg aaggatttat
     2761 taagcgttat cacgaattgc cggtcagcga attatggctg gtggatgtcg aaggtggtaa
     2821 agcgaaactg gatattattt ttgatctctg ccaacggatg attgataacg ctggcgtccc
     2881 gatgaagctt tataaaacgc tggatcgccg cgaagcattg aaagatgctg atttcgttac
     2941 tacccaactg cgcgttggcc aattaccggc gcgggaactg gatgaacgta ttccattaag
     3001 tcatggttat cttggtcagg aaactaacgg cgcgggcggt ctgtttaaag gtctgcgtac
     3061 cattccggtg atttttgaca tcgtaaaaga tgtcgaagaa ctatgtccga atgcatgggt
     3121 gattaacttc actaacccgg cgggaatggt cactgaagcc gtttatcgtc ataccggatt
     3181 taaacgcttt atcggcgtgt gtaatattcc gatcggcatg aagatgttta ttcgcgatgt
     3241 tctgatgctg aaagacagcg atgatttatc tatcgatctg ttcggcctca accatatggt
     3301 gttcattaag gatgtgctgg taaatggcaa atcacgcttt gccgaattgc ttgatggtgt
     3361 ggcatccggg cagttaaaag catctggcgt taaaaatatt ttcgatctgc catttagcga
     3421 aggcttaatt cgttctatga atctgttgcc gtgttcttat ttgctttatt acttcaagca
     3481 aaaagagatg ctggctattg aaatgggcga atactacaaa ggcggcgcac gagcgcaggt
     3541 agtacagaaa gtcgagaaac aactttttga gctatataaa aacccggagt tgaacgttaa
     3601 gccgaaagaa ctggaacagc gcggtggggc ttattattct gatgctgcgt gcgaagtgat
     3661 caacgctatc tataacgaca agcaagcaga acattacgtt aatatcccgc atcatgggca
     3721 tattgataat attccggcag actgggcggt agaaatgacc tgtacgctgg ggcgcgatgg
     3781 cgcgacgcca catccgcgca ttacgcattt cgatgataaa gtgatggggc tgattcacac
     3841 catcaaaggc ttcgagattg ctgccagcaa cgccgcactt agtggagaat ttaacgatgt
     3901 cttactggcg ctaaacctta gtccgttggt gcattccgat cgcgatgctg agctgctggc
     3961 acgcgagatg attctggcgc acgagaaatg gctgccaaat tttgccgact gcatcgcaga
     4021 gcttaaaaaa gcacattaac cgaggctgat tatggaacgc ttactgattg ttaatgccga
     4081 tgattttggc ttaagcaaag gtcagaacta cggcattatc gaggcctgtc gcaatgggat
     4141 tgtcacgtcg acgacggcgc tggtgaatgg gcaggctatt gaccatgcgg tgcagttgag
     4201 ccgtgatgaa ccgagtctgg ccatagggat gcactttgtc cttactatgg gcaagcctct
     4261 gacagctatg ccggggttaa cccgcgatgg tgtgctggga aaatggatct ggcagttggc
     4321 agaagaagat gctttaccgc tggaagaaat tactcaggag ctcgccagtc agtatttgcg
     4381 tttcattgag ctatttggac gcaaacctac gcatcttgat agccatcatc atgtgcatat
     4441 gttcccgcag attttcccga ttgtggcaaa gtttgtggct gaagagggga ttgcgttgcg
     4501 catcgaccgt caaccgctat ctaacgatgg tgatttaccg gctaatctgc gcagttcgca
     4561 gggattcagt agcgcgtttt atggtgaaga gattagcgag gcattgttcc tgcaagtgct
     4621 tgatgattcc agccatcgtg gggaacgctc gttggaagtt atgtgccatc cggcgtttgt
     4681 cgataatacg attcgccaga gtgcttattg cttccctcgg ttaacggaac tgcatgtgct
     4741 gacttcagcg tcgttgaaat atgcgattgc tgagcgtggt tatcggttgg gtagttatca
     4801 tgatgtgtaa gtaaggtggt aaggtcactc cccagggggc gtgaccttat ccatatttac
     4861 gccgggattt tgtcaatctt aggaatgcgt gaccacacgc ggtgtgctgt catcagcgtt
     4921 aacagttcat ccataaaact accagaggcg cagtcagctt ccgcaatccc ttcttcaccc
     4981 tggtcagcga ccttgattgt tgctttaaac ttgcgcgcgt ctcctgccag cgcaatcggt
     5041 ttaaggtgtt tgtaggcttc catcagatag tagttggcat cgccgttgtt agcgatatcc
     5101 gcaatattgc cgcaagggac aatgactgca tcgaccgtca gcgaaggcgc accggcaaag
     5161 gtagcagcta tagacaacac cgtaccgtca tccgcactca cttcccccat tcgggagtag
     5221 agcagtttgg catgaacgcc tttggccttc agtgccttga gaatggccag aaggtctgcc
     5281 gatctcactt catcattaag caaaattgct accacgcgac ctttcacatc accgtcagga
     5341 atggcgtaca gacttaagga tggatccttt ttcagaccgt tgacgtccgg aggtggggtg
     5401 atattcagct ggtcatcagt cagttcgata ccgagatttt tcgccaccgc ctgggccaga
     5461 gtgagatcaa tatgcgccag ctggtcaaca acgcgctcac gaatatacgg acgaactact
     5521 ttgcttaact caaaactgaa accatcgaca atatggcgct gttcgaatgg cgtctgactt
     5581 agccagaaca gacgcggatg ggaataatat tcgccaaacg atggactgcg ctcgcgaact
     5641 ttattgcctt ccacgcgttc ctggtatgat tcaaaaccgc cgcgtttcgg ccccggcggt
     5701 gtttcgcgcg gccagttatc gttgatcgag ttcggttcgt aattcgccgg gttagtgtcg
     5761 atccccatac gatgcatgcc gtcacgctgg aaattatggt aagagcaggt cgggcggtta
     5821 atcggaattt catggaaatt cggcccacca agacgactga tttgtgtatc ggtataagag
     5881 aacaaacgcc cctgcaataa cgggtcgttg gtgaaatcca gaccggggac aatatgccct
     5941 ggatggaaag ccacctgttc gttttcagca aagaagttat ccggattacg attgagcacc
     6001 attttgccga cacgctgaac gggcaccaat tcttccggga taagtttggt tggatcgaga
     6061 agatcgaagt cgaacttgaa ttcgccttct tcaggaatca actggaagcc cagttcgtat
     6121 tcaggaaaat cgcctgcttc aatcgcttcc cacaactcgc ggcggtggaa gtccgggtca
     6181 cgtccggtta atttttgtgc ttcatcccaa acgagtgagg ctttacctgc cagtggtttc
     6241 cagtggaaac gtacgaacgt tgccttccct tcggcattaa tcaggcggaa ggtatgaata
     6301 ccgaagcctt ccatagtgcg gtaactgcgc gggataccgc gatccgacat tgcccacatc
     6361 acgttgtgca gagtttcagg ttgcagagaa acataatccc agaaagtatc gtgggcgctt
     6421 tgcccttgtg gaattgccca gtgcggttct ggttttaccg catgaacaaa atcggggaat
     6481 ttatgtgcat cctggataaa gaagattggc gtgttattgc caacgaggtc aaaaataccc
     6541 tcttcagtat agaacttggt ggcaaagcca cggatatcac gtacggtatc ggcagagcca
     6601 gcgccgccct gaacggtaga gaacctaaca aatactgggg tgattttgtt cgtatctgag
     6661 aggaaatccg ctttggtgat atcgcttaag cttttatatg gctggaaata accgtgagct
     6721 gctgatccgc gtgcatgaac aatacgttcc gggatgcgct catggtcaaa gtgagtgatt
     6781 ttctcgcgca gaataaaatc ttccagcaga gttggaccac ggctaccggc acgcagtgag
     6841 ttttgatcgt cggcgatgcg cacgccctga ttagtggtca gcgcataatt ttcactgcct
     6901 ttgcgtacgt cttccagaga attaagtttt tcgttacgcg tatcaggggc tttcaggctc
     6961 cctggggcgg taggttgtgc accaggcggt gttggttcag ccgctggacg atgagatccg
     7021 tcttcaggtg ccagtgagtc catccccggt ttcgcttcgc tggaatcgtg tagtggtgac
     7081 tggtgctgat gtgggttctt ttcgttatgt tgcgacattg aactcgtctc cttaaattat
     7141 tactgaaagg gccgctgttt tggtcaaaaa accctctaac tatagaccac cagccagatc
     7201 ccgcttcgga aactattctg gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag
     7261 gggctaaatc ggctaaacta cagtttttct gatttttaat atgcgtttag tgaagccagt
     7321 aatgaagaaa ccgctccgtc agcaaaaccg ccagattatt agctatgtcc cacgcacgga
     7381 acccgcgccg ccagaacatg cgataaagat ggattcgttt cgagatgtct ggatgctgcg
     7441 tggcaaatat gttgcgtttg tactgatggg ggagtcattt ctgcgctcac cggcgtttac
     7501 tgtgcctgaa tccgcccagc gttgggcaaa tcagattcgt caggaaaatg aggtcgaaga
     7561 ataaaaaaac acctgtcggg atgacaggcg tttaacgtaa caaatcagga ttaacggtgt
     7621 gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt cagccactgg
     7681 ctggtcagcg tgccagcggt catcgagcca ctaacgttta gcgccgtacg acccatgtcg
     7741 ataagcggtt caacggagat taacaatgcc accagcgtta ccggcaggcc catcgcaggc
     7801 agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc tgcggaacta
     7861 acggtgacaa taccgaccag cgtcgcaatc cacatcgggt ccagtgggtt aatgcccacc
     7921 gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcacaacc gttctgacca
     7981 atggtcgcac cgaaagaggc ggcgaaactg gtgatggatt caggaacacc cagacgacgc
     8041 gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt aaaggcaaac
     8101 gtcagtactg gccatacctt acggaagtac ttcagcgggc tcacgccatt aatgcccagc
     8161 agaaggccat gcactgcaaa cataatcagc agaccgaggt aggacgcaac aacgaaactt
     8221 cccagtttga tgatgtcttg caggttagaa cctgcaacca ctttggtcat cagcgccaga
     8281 acaccgtaag gggtcaactg catgaccagg cgaaccagtt tcatcaccca gctttgcagg
     8341 gtatcgatag cggttaagac gcgttcacct ttcggcgcat catccttcag cagtttcagc
     8401 gcagctacgc cgaggaatgc ggcaaaaatt accacgctga taattgacgt cgaattggct
     8461 ccggtcagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg cggaacgctc
     8521 aggtcagaga ctttaccaac atagttactt tcgatggcgt tcagacgtgc agtttctgca
     8581 ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag cacaccgacc
     8641 agcgctgcaa tcagcgtggt aaacaaaagc gtaccgatgg tcagaaaact gattttgcct
     8701 aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa gactagcggc
     8761 ataacgatca tttgcagcag ttgaacatag ccgttaccaa caatgttaaa ccactgtaca
     8821 gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag gccaaaaacc
     8881 acacccatca caagacccac cagcactttt ttcgccagac tccactgttt gtggcgggtt
     8941 tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa tggaaagttc
     9001 atccccgttc tcctgatctt attatttcta tcactgcgta acagtcatag tgttggcgca
     9061 agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat tccagaaagg
     9121 cgatttttgc ctttttagta ttcaacctga tgcttaataa gccgatgaaa ctggatctgt
     9181 aaggtattga tcatttgcga tgaccaacga acagcgctgt tttcgggtaa tgaatggggc
     9241 atccataccg accagacaaa taatgccatg cagataaaac gttccagttg gttgcccttt
     9301 tggggaacca ggataggcaa gcggaaacgc cagcggcatg gccagagcag gggaacaccg
     9361 gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac ccagcaccat accctgcaac
     9421 gcatcagccg gaatgaacca gctttcagga actttaaggt aaaaggttgc cagcagcgca
     9481 aataccgcca gtagactgtg ggtaaaacca cgatgcccaa aagcgcgggc gatcggtttt
     9541 gatatccact ttaatcgctg cccaagaaac gactttgggt gatcgatgtc cggtaacaaa
     9601 cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag cacgggcgtc
     9661 agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg accttccgcc
     9721 gtcatgataa aactcactaa actgtcgata cgtacagtat agggttttta tacagtgcac
     9781 ggaagtggtg acggtgtaac tctttctctt aaattggcga ttaatccgtc agttgaatgc
     9841 aaattcagat aaagaaccag gggcagtgtg atcactgccc cctggtcatt aaccgagaag
     9901 gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg
     9961 atcattttgc gcttctggcg caggaacgac gatggaacgc atgcgggctg ctttagaggc
    10021 gatcatgcca tttaccgaat cttccagtgc cacgcaggtc agagggtcaa cccccagttt
    10081 tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcaatt tttcagccga
    10141 ggcgagggca tcgaaactgt cgcgtaaatc aaacatggtc aatacttttt ccagcatatg
    10201 cagcggtgac gcggaggcca gccccaccaa taaaccttgt tctttgcata acgcaacggc
    10261 ttcacgcaca cctggcaata atggacgtgt ctcttcaatc agtgaaatgg cacgggcaat
    10321 aacttgttct actacttcct gacggcttgg cccattccat ggttgccggg cgtaccaaag
    10381 atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc agctcgttac gacgggagat
    10441 atccaccccc aggcttgcca tcacatccag ttcggctcga tcccataaag gttctgagtc
    10501 gataagtaat ccatccatat caaaaattgc agcaagaatc tgacgcgggg ttgacattac
    10561 aacctctcct ttagagcatt atatgtaagg tattaagccg tgcttatgat taagcgccta
    10621 tcatatcgcc tttaaagaat gggcgaaaac agatatctac aggtaaacta agtgaaacga
    10681 cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt gctgttttga
    10741 aaaggatttt gggttgggtg atttttatac ctgcattgat ctctacattg atttctttgc
    10801 tgaaatttat gaatacccgg caggaaaacc aggaaggcat taatgcggtc atgctcgact
    10861 ttactcatgt catgatcgat atgatgcagg cgaatacgcc ttttttgaat ctgttctggt
    10921 acaactctcc gacgcccaat tttaatggtg gcgtgaacgt tatgttctgg gtgattttca
    10981 tcctgatttt tgtcggactg gcgttgcagg attccggagc ccggatgagt cgccaggcgc
    11041 gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa ggggaagagg
    11101 ggctaacgcg tgaacaaatt gagtcacgta ttgttgttcc gcaccatact attttcctgc
    11161 agtttttctc gctgtatatc ctgccggtta tttgtattgc tgcaggttat gtgttctttt
    11221 ctctgcttgg gtttatttaa tcaaaaggcc gcacggagcg gccattatcc tcaacccata
    11281 tcatgctgct aataatctat ccagtgactg ctgagcaata accaaatgct gaccgccaaa
    11341 taaccttgca cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt caaggaaatc
    11401 ggcaggtagc ggggatactg actgatagcc gtcatagatt tgtggcggct gttcagtatg
    11461 cagcggtaac atcgccaggt cgcactctcg gtcaccccag taacaggccg ggtcgaaaat
    11521 gtacgggcca tccggaccca gcgcacagtt gccggaccat aaatcgccgt gcaacagaga
    11581 aggctgcggt tgatgcgagg cgagacgctg ctgaatatgc tcgacgatgg catcgatatt
    11641 gccgaaagcg atcccttttt ctgccgccag ttccagctgc cagccaatcc gttgttcagc
    11701 aaaaaaagtt gaccagcgac gttgccaggt gttgggctgt ggagttgtgg agagcgcgtt
    11761 atcgaaatcg aggccaaatt gtggttggtc gctccattga tgtaaacgcg caatttgctg
    11821 accaagaata aatgcactat gcgcatccag cgggcgaggt gggagataat ccatcaccag
    11881 aaaactgtag tcacggtcag cgccaactgc ccaaacctta ggcacggtga cggttttact
    11941 acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca gttccctttc
    12001 atcacatttg acgaaaaagt cacgtcctgc atagcgcaaa tgccatgcgg catggacttc
    12061 tccgccaggc agttcattac gcagttcgat ttcgccttca cctaactgct cgctcaaaag
    12121 acgactaatt gcctgccaca tgatgtctct cccatgttgt ctgccagatc ataaggttag
    12181 cgcaagaatg cggtgtaaaa acacgagcta acgcacacca cagactgatt attgcccaaa
    12241 aaccatttat tttctgttgc tattccattc ctcccaggtc gtgatggtga tttcaggatc
    12301 tttgccggtt gcactgtggg taagccccga aaccagttct ctaatttctt cttcactttg
    12361 ggtactgata agtccaaaag tgttagtccc cagctcatgg atatttccat catcatccgt
    12421 catggtgagc aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa tttcagtcaa
    12481 tgtatcctca tgcaacatga cggttattac ataacggaca agatcgccac tggccatagt
    12541 tcaccccttt gttatcacat gacttttgag catagtcgga gaaacgcgtt gccgacaggt
    12601 tggtggtgat tcccccaatg ctgggggaat gtttttgtta gcgggaaagg tacgcgtaaa
    12661 ttttttgcgt atcgtcatgg gagcacaggc gtgttccctg gttgagtgtg gctgcactcc
    12721 ccgcagctac gccaaaacgg accatctctt caagagaggc attttctgcc agtttaagtg
    12781 tcatcgcgcc gaccatgctg tcaccagcgc caacggtact ctggcttttc accggtggtg
    12841 gcaccacctg aatgcagttt tcactatcaa cgcccagcgc cccttgtgga cccagggaaa
    12901 cgacaacccg tttggccttg ccgctattaa cgatttcctg cgcggctttg cggacatcgt
    12961 ctggctgggt gagttcgcga tttaccagcg cactgagttc tttttggtta ggcttaacca
    13021 actcgatgtt accaatcgcc agtgctgcac ttaacgcttc gccagaactg tcgacgatgc
    13081 agcggatccc ttgtttttgc gcagcggaaa tcagttgggt taatttttcc agcttcacac
    13141 ctggcggcag gcttccgctt atgaccagga tggccccgga ttcaatttcc agaacttgct
    13201 cttcgagctg gcgaaactca tcttcattta atgccgcgcc tggcataaca aaacgatact
    13261 gctcaccgct tgcttccaca tgtacgtgta aattctgccg ggtccagtct ttggcttcta
    13321 cagtagcgac ggggacattt tcatgcgcca acagtgaaac cagatgttcg ccggtcgcgc
    13381 cacccgccgg gaagatcgct gtggcagtgc ctccaagatg ggcaatggcg cgggcgacat
    13441 tgatgccgcc gccaccgggt tcgaacaccg gtgcggtaca gcgcagtttt ccttcgggat
    13501 aaatttgcgg ggtaattgtt gcgctatcaa gagagggcgc aagtgtcaac gtatagatac
    13561 gtaccatcat ttcctcctat aggctgattt cagtctggca ccgaatcagc ggaaagtgaa
    13621 gaaattaaca a
//
LOCUS       DAZFRG010000125        13604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFRG010000125 DAZFRG010000000
VERSION     DAZFRG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.125"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(352..777)
                     /gene="arsC"
                     /locus_tag="ACHKUC_002970"
     CDS             complement(352..777)
                     /gene="arsC"
                     /locus_tag="ACHKUC_002970"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO6619194.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEKLGLAEDKFTHDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1112..1195
                     /gene="dinQ"
                     /locus_tag="ACHKUC_002971"
     CDS             1112..1195
                     /gene="dinQ"
                     /locus_tag="ACHKUC_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO6619195.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1249..2601)
                     /gene="gorA"
                     /locus_tag="ACHKUC_002972"
     CDS             complement(1249..2601)
                     /gene="gorA"
                     /locus_tag="ACHKUC_002972"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO6619196.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2673..3515)
                     /gene="rlmJ"
                     /locus_tag="ACHKUC_002973"
     CDS             complement(2673..3515)
                     /gene="rlmJ"
                     /locus_tag="ACHKUC_002973"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO6619197.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3718..5760
                     /gene="prlC"
                     /locus_tag="ACHKUC_002974"
     CDS             3718..5760
                     /gene="prlC"
                     /locus_tag="ACHKUC_002974"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO6619198.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENDELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5768..6520
                     /gene="rsmJ"
                     /locus_tag="ACHKUC_002975"
     CDS             5768..6520
                     /gene="rsmJ"
                     /locus_tag="ACHKUC_002975"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO6619199.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLAIKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6569..8038)
                     /gene="dtpB"
                     /locus_tag="ACHKUC_002976"
     CDS             complement(6569..8038)
                     /gene="dtpB"
                     /locus_tag="ACHKUC_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO6619200.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8204..8265
                     /locus_tag="ACHKUC_002977"
                     /pseudo
     CDS             8204..8265
                     /locus_tag="ACHKUC_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8355..8789)
                     /gene="uspA"
                     /locus_tag="ACHKUC_002978"
     CDS             complement(8355..8789)
                     /gene="uspA"
                     /locus_tag="ACHKUC_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO6619201.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9180..9515
                     /gene="uspB"
                     /locus_tag="ACHKUC_002979"
     CDS             9180..9515
                     /gene="uspB"
                     /locus_tag="ACHKUC_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO6619202.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9586..11085)
                     /gene="pitA"
                     /locus_tag="ACHKUC_002980"
     CDS             complement(9586..11085)
                     /gene="pitA"
                     /locus_tag="ACHKUC_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO6619203.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11317..12510
                     /gene="yhiN"
                     /locus_tag="ACHKUC_002981"
     CDS             11317..12510
                     /gene="yhiN"
                     /locus_tag="ACHKUC_002981"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO6619204.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDLSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12597..>13604)
                     /gene="yhiM"
                     /locus_tag="ACHKUC_002982"
     CDS             complement(12597..>13604)
                     /gene="yhiM"
                     /locus_tag="ACHKUC_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO6619205.1"
                     /translation="MGIICIALGEFVLTGSGQSEYFVAGHVLISLSAICLALFTTAFI
                     IISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVGMI
                     AACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIWSV
                     ILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVILL
                     GSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRI
                     PMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGSAK
                     K"
BASE COUNT         3470 a         3446 c         3580 g         3108 t
ORIGIN      
        1 tttgtgatgt tttagatatg taataataaa ataggttaac gtatgttgaa attatttatt
       61 gtgtaaacca ctgataaatc ataatccagt acatctttaa actgctgcac tgtatcgctg
      121 ataactaagc agctcactga tacaccatgc tggacgcgat aattggaatc tcacgcaggg
      181 agtactgtgt aaatagcaaa attgcctgat gcgctacgac tgtcaggcct acaaataaca
      241 tacaattcat cgaattatca taacgttgta ggtcggataa gacgcgccag cgtcgcatcc
      301 gacataaata aagcgcattt ttctaacaac ctgtgggggg atatagccgc tttatttcag
      361 gcgattaccc gcctcatcaa ccactttctc gccatcttcc ttggtgaacg cgcctttttg
      421 cgcatctggc agaatttcca gcacgacttc tgaagggcgg cacaggcgag ttcccagcgg
      481 cgtcaccaca atcgggcgat taataagaat cgggtgctga agcataaagt cgattaactg
      541 atcgtgagta aatttatctt ctgcaaggcc cagtttctca tacggctcga cgtttttacg
      601 cagcaacgcg cgtacggtaa tccccatatc ggcaatgagt tttaccagct catcgcgcgt
      661 cggtggtgtt tccagataat agataatggt cggttctgta ccgctgttgc ggatcatctc
      721 cagcgtatta cgcgacgtac cgcaggccgg gttgtgataa atggtaatgt tgctcatatc
      781 agtatctcat tacgatgtga aagagaggtg tagcgccagc gccaccagcg ttacaaacca
      841 aagcggacaa gtcaaaaaca catatggttt aacgaatatg taagtgtaga gaaaggtgta
      901 ggtgtgaatt aagcgttggg cattcatttg aaaggaggtc tgaatcagga cgtaaaaaaa
      961 ccgacttcgc gtcggttttt ttactgttca gccctgagtt ggtggctctg gctggagtga
     1021 gatagcccac atctaacaat gagacatgcg ccgtgacagg cagtggatga gtaagcggat
     1081 gcattctcac tccatcgcat ggagaaaacg ggtgattgat aaagcaatca tcgttcttgg
     1141 ggcgttaatt gcgctgctgg aactgatccg ctttctgctt cagcttctga actgatagcg
     1201 gaaacgtagc taagggctaa gagcacacta ctcttagccc tttaacattt aacgcattgt
     1261 cacgaactct tctgccgctg tcgggtgaat ggcaaccgtg ttgtcgaagt cttttttggt
     1321 tgcacccatc ttcagcgcca ccgcgaagcc ctgcaacatt tcgtccatac caaaaccaat
     1381 gccgtgaata ccgacaatct tctcttccgg tccaacgcat accagtttca tgcggcacgg
     1441 ctggcggtga gtggtgacgg cggtatacat cgcggtgaaa gaggatttat acactttcac
     1501 ctgatcgtcg ccgtactgct cgcgcgcctg cggctccgtt aaaccaacag taccaatcgg
     1561 cggatggctg aagaccacgg tcggaatgtt gctgtaatcc agatgctcat ccggcttgtt
     1621 attaaacagg cgttcagaga gacggcgacc tgctgcaaca gccaccggtg tcagttccac
     1681 tgcacccgtg ttatcgccca ccgcgtaaat accttcaacg ttggtgtttt gatatttatc
     1741 gacgacgata taaccttttt cgttagtttt aacgccagcg gcttccaggt tgatgttgtc
     1801 attggcaggc tcgcgaccaa tcgcccaaat caggcaatcc accgtttcac tgcgaccatc
     1861 ttccagctcc agcgtcaggc taccatcggc atttttcact accgctttcg ggatggcgtt
     1921 ggtgtgcaac tgcgggcctt cggcgttcat cacttcaacc agcgtttcag agatcatcgg
     1981 gtcgaagctg cgcagcggcg catgtttacg cacaaacaga tgcgtcttcg caccgaggcc
     2041 gttaatcacg cccgccagtt caacggcgat gtaacccgcg ccaacaaccg ctacacgctc
     2101 tggcaaagcc ggaagggcga agaagccatc agaatcaata ccgtattcca cgcctggaat
     2161 atccgggtgg ctcggacgac caccggtggc gatcagaata tgatcggccg tgatggtttc
     2221 gccgttgact tcaatggttt tggcatcaac gaagcgggca aagcctttga ttacatcaac
     2281 gttattttta ccgagcacgt tctcatagga agtatgaata cggtcgatat aggcggtacg
     2341 gctggcgatc aacgtttccc agttgaattt attgatagtg gtatcaaaac cataatccgg
     2401 gccgtacata tggatcgctt cacggatttg cgccgcgtgc cacatcactt ttttcggcac
     2461 acagccaaca tttacgcagg tgccgcccag ctctttggct tcaatcagtg cgcatttctg
     2521 gccgtacata gccgcgcggt tgatggaggc gataccgccg ctgccgccgc cgatggcgat
     2581 gtaatcatag tgtttagtca tgacatattg tccttatcgt tgattaccgc gattgtagcg
     2641 cgagacgtaa taggtgccag caatggctgc aattactctg gcacgatcca gcttacggtg
     2701 gcgtgcccgg tgcctgccgg aaccagtttg ctgtgcagcc acggcagcac gttattcatc
     2761 tgctgttcca gcttccacgg cgggttaatc acaatcatgc cagaagcggt catgccacgg
     2821 cgatcgctgt ctggcagcac cgccagttca atttgcagaa ttttgcgaat gccggtcgct
     2881 tccaggtcgt ggatcatgcg cttaatttgc tggcgcagca ccactggata ccacaatgcg
     2941 taagtgccag tggcgaaacg tttgtaacct tctgctatcc cgctgaccac cgcctgatag
     3001 tcggttttca tttcatacgg cgggtcgata aggattaaac cacggcggga aaccggcggc
     3061 agcttggcct taagctgctg gaaaccgtcg gctttttcga cgcgcgcacg gctgtctttc
     3121 tgaaattcag aacgcagcaa cgggtaatcg ctcgggtgca gttcggtcag ttgcaggctg
     3181 tcctgttcac gcagtagctg gcgcgcaatc aacggcgaac cggggtaata acgcaactgc
     3241 ccgctacggt tgaagtgttt taccacattg atgtacgcct ccagctccgc aggcaaatcg
     3301 tcctgctgcc agatacgggc gatgccttcg agatattcgc cggtacgctc ggcatgttcg
     3361 ctgcctaact gataacgccc ggcccctgcg tgggtgtcga gatagagaaa cggtttatct
     3421 ttctctttca gcgactcaat gatcaggctc tgaacggtat gtttaaggac gtcggcgtgg
     3481 ttgccagcgt gaaagctgtg gcgataactg agcatgggta aaggtgttcc ggtaagtaaa
     3541 aatcggcccg tattcggggc gcacaaaagc ctatcaggac agtataccga aaagaggccg
     3601 ccgccgcgaa agcgtaacgt ttctcattga aattcactac acttaacccc atgctacaaa
     3661 cattatgtaa agcgcctgtt gagcgcttcc ttaacctctt taaccaggac tgcgctaatg
     3721 acgaatccgt tactgactcc ctttgaattg cctccgtttt ctaaaattct cccggaacat
     3781 gtcgttccag ccgtgactaa ggcgctgaac gactgccgcg aaaatgtgga gcgcgtagta
     3841 gcgcaagggg caccgtacac ctgggaaaat ctctgccagc cgttggcgga agtggacgat
     3901 gtgctggggc gtatcttctc cccggtcagc catctgaact cggtgaaaaa tagcccggaa
     3961 ctgcgtgaag cgtacgaaca aaccctgccg ctgctgtcag aatacagcac ctgggtaggg
     4021 caacatgaag ggctgtataa agcatatcgc gacctgcgcg atggcgatca ttacgccacg
     4081 ctgaacacgg cgcagaaaaa agcggttgat aacgcactgc gcgatttcga actctctggc
     4141 atcggtctgc cgaaagagaa acagcagcgt tacggcgaaa ttgctacccg tctttctgaa
     4201 ctgggcaatc agtacagcaa caacgtcctc gatgcgacga tgggctggac caaactcgtt
     4261 accgacgaag cggagctggc gggaatgcct gaaagcgcgc tggctgcggc aaaagcccag
     4321 gccgaagcga aagagctgga aggttatttg ctgacgctgg atatcccaag ctacctgccg
     4381 gtaatgacct actgcgacaa ccaggctctg cgcgaagaga tgtatcgcgc ttatagcact
     4441 cgtgcttccg atcaaggtcc gaacgccggt aagtgggata acagcaaggt gatggaagag
     4501 atcctcgcgc tgcgtcacga actggcgcaa ctgctgggct ttgaaaacta cgccttcaaa
     4561 tcacttgcca ctaaaatggc agaaaacccg cagcaggtgc tggatttctt aaccgatctg
     4621 gcaaaacgcg cgcgcccgca aggcgaaaaa gagctggcgc aactgcgcgc cttcgccaaa
     4681 gccgaatttg gcgtcgatga gttgcaaccg tgggatatcg cttactacag cgaaaaacag
     4741 aaacagcacc tctacagcat cagcgatgaa caactgcgtc cgtacttccc ggaaaacaaa
     4801 gcggttaacg gcctgtttga agtggtgaaa cgtatttacg gcatcaccgc taaagagcgt
     4861 aaagatgttg atgtctggca tccggatgta cgtttcttcg agctgtatga cgagaacgac
     4921 gaactgcgcg gcagcttcta cctcgacctg tatgcccgtg aaaacaaacg cggcggggcg
     4981 tggatggatg actgcgtagg ccagatgcgt aaagccgacg gttcgctgca aaaaccggtc
     5041 gcgtatctga cctgcaactt caaccgcccg gtaaatggta aaccggcgct gtttacccat
     5101 gacgaagtga tcaccctgtt ccacgagttc ggtcacggcc tgcaccatat gctgacccgc
     5161 attgaaaccg ctggtgtttc cgggatcagt ggggtgccgt gggatgcggt cgaactgccg
     5221 agccagttta tggaaaactg gtgctgggag ccggaggcgc tggcgtttat ctccggtcac
     5281 tatgaaaccg gcgagccgct gccgaaggag ctgctggata aaatgctggc agcgaaaaat
     5341 taccaggcgg cgctgtttat tctgcgccag ctggagttcg gcctgttcga tttccgcctc
     5401 catgcagagt tccgtccgga tcagggggcg aaaatcctcg aaactctggc agaaatcaag
     5461 aaactggttg ccgtggtacc gtctccgtca tggggccgtt tcccgcatgc tttcagccac
     5521 atttttgccg gtggttatgc ggcaggttac tacagctacc tgtgggccga tgtgctggcg
     5581 gcagatgcct tctcgcgttt tgaggaagag ggcattttca accgtgaaac tgggcaatcg
     5641 ttcctcgaca acattctgag ccgtggcggt tcagaagagc cgatggagct gttcaaacgc
     5701 ttccgtggtc gtgaaccaca gctggatgcg atgctggagc attacggcat taagggctga
     5761 acattcagtg aaaatctgct taattgatga aacaggcgcc ggagacggcg ccttatctgt
     5821 tctggcggcc cgctgggggc tggaacacga tgaagacaac ctgatggcgc tggtgttaac
     5881 gccggagcat ctggaactgc gcaagcgtga tgagccgaaa cttggcggca tctttgttga
     5941 ttttgttggc ggagcgatgg cgcaccgacg caaattcggc ggcggacgtg gtgaagcggt
     6001 ggcaaaagca gtgggcatta aaggcgatta tttgccggat gtggtggacg ccaccgcagg
     6061 attagggcgc gatgcctttg tgctggcttc ggtcggctgc cgcgtgcgga tgctggagcg
     6121 caatccggtg gttgccgcgt tgctcgacga tggtctggcg cgtggttatg cggatgcgga
     6181 aatcggcggc tggttacagg aacgtttgca gttgattcac gcctccagtc tgacggcgct
     6241 gagcgatatc accccgcgtc cgcaggtagt ttatcttgac ccaatgttcc cgcataagca
     6301 gaaaagcgcg ctggtgaaaa aagagatgcg cgtgtttcag tcgctggtgg ggccggatct
     6361 tgatgccgat ggattgttgg agcctgcgcg gttgctggca atcaaacgcg tggtggtgaa
     6421 gcgtccggac tatgcgccgc cgctggcgaa tgtcgccacg ccaaacgcgg tagtcactaa
     6481 agggcatcgc tttgatattt atgccggaac gccggtgtaa aagtaaagcg gggtagcgat
     6541 aacgctaccc cgctttctgc aagaataatt aatggctttc cggcgtcgca atcatgcgtt
     6601 tcagccacgg caccatcaat agcatcacta ctgcaacgcc cagcgtaacc aggccaatct
     6661 taccaaacac gttggtatag acgggcaacg tctcaagtgg atcggtaatg ttgtctggta
     6721 ctgcggtaaa tgttgccaca tagccgccca gcaagaacgc ggcagcctgc gtcaggaacc
     6781 acatcccgag aataaagccc atcaaatgct gcggcaccag ggcagcaatc atcgccaggc
     6841 caagggcgct aataaacagt tcacctaagc tctggaataa gtacaccagc acgataaacc
     6901 atggcgatgt cagcccctgg gcatccgcaa accacattcc agccgctgca gccgtcaaaa
     6961 agcccagtga gcacataaac atgccgagag taaacttcat cggcatcgag agatctttgc
     7021 ctttgttacc cagatgcgtg taaatgcctg ccagtattgg gctggcgagt accacccaga
     7081 acgggttaag cgcctggaag ctgaccgggt tgatggaaaa gccgagaatt tcatgatgca
     7141 cgttgttgat ggcaaagaag ttcagcgacg ttggcatctg ggcgtagaga atgtaaaaca
     7201 ccaccgcttc gagcatcagg acaaaggcga caaacatttt attgcgcccg gttttatcca
     7261 gcttgaatgc ctgacgaaag aagatgatgg tgacgacgat ggagagaaca atcagaacca
     7321 gattggcgac ttctacgttg tgcatcagcc aggcgcagac gaagatcatc accacgctgc
     7381 caagtaatac gtacaacagt ttactgaagc tcatcggccg gaagtcgggt tcagaaccaa
     7441 tgtctttcac cattccacga caggcgatgt aaaccagtaa tgcgataatt aaccctgcac
     7501 cgcacaggtt gtaggtgact gaatagccga atttatcagc gatcacaggg gccagcgata
     7561 acgctatcaa cgagccgata ttgatcgaca tatagaacag ggtgaatgcg ccatcaagcc
     7621 gcggatcttt cggcgggtag cacttcgaaa gcaagctggc tgggttagct ttaaacaggc
     7681 cgttaccgac agcgatagtc cccagggcga tgaaaatcag gtcaggctta agtagcgaca
     7741 tgccggtcat gaagtagcca atcgccagca caagtgctcc gagaacaatg gtgcgtttgg
     7801 ttcccagcag gtggtcgcca acatagccac caatggaaat gaggccatag accagcgcag
     7861 caaaagcacc aaaagtgaca aaagcctgct cttgcgagaa cccaagctgt ttaacgaaga
     7921 aaaccgccag tacgccctgc acgccgtagt agccgaatcg ctcccataac tcgacaaaaa
     7981 agatcatgaa aaatgggcga ggttgctgca gcatccccat gggtgttgtt gtattcatat
     8041 ttattaacct tccaatacca tcctgaaaag tattaacgcg taagggataa cgcgcgaaaa
     8101 agtttacatc tgtcagagat tcttaatatt gatgcggaga atagattaat acactattac
     8161 aacagaaaat aaccagctga tcacactaat gatagaaaat ataatggtgt taaatgttgt
     8221 attggcaggg gttttgattg cgtacgcaga ccgcaggcca gataaggcgt ttacgctgca
     8281 tcaggtaaca aggcgcgcca aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg
     8341 tcgtggaggg aagattattc ttcttcgtcg cgcagcggaa caatcagcat atcaacgtga
     8401 acggtgttga tcagctgacg tgcggaagac atcagtttgc tccagaagtc ctggtggtga
     8461 ccacaaacca ccagatccat atcgtatttc ttgattgcat cgaccagtac ctggcccaga
     8521 tcgccgctgc cgctcagggt ttcagtgatt gggtagcctg cattggttga aagctcggtc
     8581 agtgcatgat gtgtctcttc agagatgcgt ttctgcatat cacccagatt cacatcgata
     8641 agcccggtgt ataggtcaga gtagtttaca tctacgtgga tcagagaaac tttcgcattg
     8701 taggggcgag ccatagagac tgctttctct accagaactt tgctttccgg ggagaggtcg
     8761 accgcgatga gaatgtgttt ataagccata gtgttactcc ttccataaag ttgtcgatga
     8821 ctggccagct agcgtttctt gtgccgttac gtcacccgcg tcctgcgaac tacgtgcgcg
     8881 gggctaaccc agccgtaaag attcagtgtt aagaccatcc ttaccttata gcgacccgga
     8941 tgatccgtca atccgccttg cttactcgtt agataaacaa attttcgaca tgaatatatt
     9001 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
     9061 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
     9121 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
     9181 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
     9241 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
     9301 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
     9361 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
     9421 gccgctgtga gcgggtgcgt cggcagttta ttctgaccag cgcattgtgt ggtctggtgg
     9481 tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa tgacgcataa
     9541 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     9601 ggagagccag tacagcccgc cggaaagcag taccgcagcc ggaagggtaa atacccaggc
     9661 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
     9721 cgcgacagaa gaggagagta cgtgagtagt ggaaaccggc atcccggtat aacttgccag
     9781 gccgatagac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     9841 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
     9901 acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag
     9961 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc caggcatctt
    10021 caccacttta tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
    10081 atcaagcgac agcttgtcgt agctttccac atcggtagtc aacatgcctt tcaggcggtt
    10141 gagcgcgtta atggtattcg acggatggca atggaactcc gcgggttgcg ttgcgccagc
    10201 ttccggagcc ggtactaact gatcagcacc ggtcgcttgt ttcagcagcg caggatgctg
    10261 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
    10321 ggcattcatg ttcaccacga agcctgctgg tgcgacacca atcaatacca acataaccag
    10381 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
    10441 gatcagcgca atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
    10501 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
    10561 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    10621 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    10681 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    10741 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    10801 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    10861 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    10921 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    10981 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    11041 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    11101 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    11161 tagacttttt ttgatttgcc tcctgtatgg attccactca aaaaataatt atcttatata
    11221 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    11281 acaattcacg cccgtttttt cttagaggag cgcaacgtgg aaaggtttga tgccattatt
    11341 ataggcgctg gtgcggcagg tatgttctgt tctgcgctgg caggtcaggc aggacgccgg
    11401 gttctgctga tcgataatgg taaaaaacca gggcgcaaaa tcctcatgtc tggcggtggg
    11461 cgctgcaact ttaccaacct ttatgtcgaa ccgggcgctt atctgagcca gaatccgcat
    11521 ttttgtaagt ctgcactcgc gcgttttacc cagtgggatt tcattgatct ggtcaataaa
    11581 cacggcatcg cctggcacga gaaaacgtta gggcagctct tctgcgatga ctccgcgcag
    11641 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtggcctt cagattgcgt
    11701 agcgaagtgc tgagcgtggc gaaggatgat acaggcttca cgcttgaact gaacggcatg
    11761 actgtcggtt gcgaaaagct ggtcattgcg accggtggac tgtcaatgcc ggggctgggc
    11821 gcatcgccgt ttggttataa gattgccgaa caattcggcc tcaacgtgct gccgacccgt
    11881 gcgggtctgg tgccattcac tctgcataaa ccgttgcttg aagagttaca ggtgctggcg
    11941 ggcgtggcgg tgccttccgt gattaccgct gaaaacggta ccgttttccg tgagaactta
    12001 ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttccag ctactggcaa
    12061 ccgggtgaat ttgtcagcat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    12121 gagcagcgta atgcacatcc gaatcaaagc ctgaaaaaca cgctggcggt tcatctgccg
    12181 aagcggttgg ttgaacggtt acaacaactc gggcaaatcc cggatctctc gctaaaacag
    12241 ctcaacgtgc gtgaccaaca gacactgatt agcacattga ctgactggcg cgtacaaccc
    12301 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgag
    12361 ctctcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg
    12421 atggacgtca ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    12481 gcttgtgcgc aggatttggt ggcagaataa gaagcaaaat gtgctctcga taccagaggg
    12541 attaacctga ataaaaccag ataaggcgtc caacgcctta tctgaagcct tttttattat
    12601 tttttcgctg aacccgcttc taatatagaa acgatggaaa acagtgtaaa acaaactgca
    12661 cctaagccaa ccagaactcg cgaaggaata aagtaagcca tatcggtttg cgcaatttca
    12721 gccaggaacg cagcaaggaa cagacaaaac agacaggtaa ataccggaat catgggtatt
    12781 ctgttggcta acgaacaggt gcgtcgccat accagcgcta acaaccacac tttggagaaa
    12841 atactgtagc agatcatccc caggcaaatg agaataatgc cgggggccag gcgggcgctt
    12901 gcatctgaac tgattaatac gtagatacca aaaataatgg taagtgagcc aagcaaaata
    12961 acccaatagc accacagcca atgttctttc actgagaacg tattccgcgt ttgatggaca
    13021 atagtggcga caaggccaat aagacaagcg caaattgccg ttaaccccat tagtacgtga
    13081 cctgcaacat aatgtggtgt gatatctgca ctacgtaaca gtatgactga ccaaatgaaa
    13141 ccaaagagag taagcagaac gggaacggca attaagcaat taccgattgt tgaagaatag
    13201 gcctgcaatg gcgtaccgtc actcttactg cccgaggcgt ttttggggat caacagaaaa
    13261 tgtccggaag atgccgctac ggttgataca caagcggcaa tcatgccaac gccgaaaata
    13321 acatgaccgg cgacaaattc atctgccatc acattattgc ttgccaacag tgaccatccc
    13381 caaatcatag ttgtagcaga accggcataa ccaataacgg gaaataacct gttgtagaat
    13441 ttattcatgc cgtgagttaa ctgagatata ataataaatg cggtagtgaa caatgccaga
    13501 catatcgcag aaagagaaat cagaacatgc cctgcaacga aatattcact ttgccctgaa
    13561 ccggttaata caaactcacc taatgcgatg catattatac ccat
//
LOCUS       DAZFRG010000126        13489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFRG010000126 DAZFRG010000000
VERSION     DAZFRG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.126"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..868)
                     /gene="tcyN"
                     /locus_tag="ACHKUC_002983"
     CDS             complement(116..868)
                     /gene="tcyN"
                     /locus_tag="ACHKUC_002983"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO6619206.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="ACHKUC_002984"
     CDS             complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="ACHKUC_002984"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO6619207.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="ACHKUC_002985"
     CDS             complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="ACHKUC_002985"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO6619208.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2639..3439)
                     /gene="tcyJ"
                     /locus_tag="ACHKUC_002986"
     CDS             complement(2639..3439)
                     /gene="tcyJ"
                     /locus_tag="ACHKUC_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6619209.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3527..4078)
                     /gene="fliZ"
                     /locus_tag="ACHKUC_002987"
     CDS             complement(3527..4078)
                     /gene="fliZ"
                     /locus_tag="ACHKUC_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO6619210.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4124..4843)
                     /gene="fliA"
                     /locus_tag="ACHKUC_002988"
                     /pseudo
     CDS             complement(4124..4843)
                     /gene="fliA"
                     /locus_tag="ACHKUC_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5163..6473)
                     /locus_tag="ACHKUC_002989"
     CDS             complement(5163..6473)
                     /locus_tag="ACHKUC_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO6619211.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSALRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTISIDL
                     QKIDSSTLGLNGFSVSGQSLNVSDSITQITGAAGTKPVGVDFTAVAKDLTTATGKTVD
                     VSSLTLHNTLDAKGAATSQFVVQSGNDFYSASINHTDGKVTLNKADVEYTDTDNGLTT
                     AATQKDQLIKVAADSDGSAAGYVTFQGKNYATTVSTALDDNTAAKATDNKVVVELSTA
                     KPTAQFSGASSADPLALLDKAIAQVDTFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6721..8136
                     /gene="fliD"
                     /locus_tag="ACHKUC_002990"
     CDS             6721..8136
                     /gene="fliD"
                     /locus_tag="ACHKUC_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6619212.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALECFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSDNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            8152..8562
                     /gene="fliS"
                     /locus_tag="ACHKUC_002991"
     CDS             8152..8562
                     /gene="fliS"
                     /locus_tag="ACHKUC_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6619213.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKASLLSPSLIQDPV"
     gene            8562..8927
                     /gene="fliT"
                     /locus_tag="ACHKUC_002992"
     CDS             8562..8927
                     /gene="fliT"
                     /locus_tag="ACHKUC_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO6619214.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLLLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9005..10492
                     /gene="amyA"
                     /locus_tag="ACHKUC_002993"
     CDS             9005..10492
                     /gene="amyA"
                     /locus_tag="ACHKUC_002993"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6619215.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10526..10939)
                     /gene="yedD"
                     /locus_tag="ACHKUC_002994"
     CDS             complement(10526..10939)
                     /gene="yedD"
                     /locus_tag="ACHKUC_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO6619216.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11126..12331
                     /gene="yedE"
                     /locus_tag="ACHKUC_002995"
     CDS             11126..12331
                     /gene="yedE"
                     /locus_tag="ACHKUC_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO6619217.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            12328..12561
                     /gene="yedF"
                     /locus_tag="ACHKUC_002996"
     CDS             12328..12561
                     /gene="yedF"
                     /locus_tag="ACHKUC_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO6619218.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12670..13338
                     /locus_tag="ACHKUC_002997"
     CDS             12670..13338
                     /locus_tag="ACHKUC_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO6619219.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGGWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFIAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3326 a         3472 c         3352 g         3339 t
ORIGIN      
        1 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
       61 gtttgaaatg gcgtcccgcc ccgactggtg aatcgtcggg gctgattttt tcttattatt
      121 gcagcagaaa cttctccagg aactggcggg tgcgaggctg ctgaggatca gcaaataacg
      181 cttttgctac cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
      241 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
      301 gctgacgaat ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct
      361 catcaaacaa aatcacttca ggacgcattg ccagagcacg cgcaatcgca acgcgctgct
      421 gttgaccgcc agacaaacga cgtggataac tggtttcttt accggccagc ccaactttcg
      481 ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
      541 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa cagattaaag ttctggaaga
      601 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
      661 cagtatcaat agtgatatcg ccaacggtga tggttcccgc ttccggttgt tccagcagat
      721 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataata gccactactt
      781 cgccaggctt cacctcaagg tcgataccat gcagcaccgt ctgaccgtgg aattttttca
      841 ccaggttctt aacttcaatg gcactcattt gggttccctc tcctggcgat taagttgatt
      901 ctcaaaatgg ttctgcaacg tcgataacac agtcgccata atccagtaga tgagcgaagc
      961 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
     1021 cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc tgataaagct
     1081 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt
     1141 ctgccatggc gtcataccaa tactggctgc agcttcccac tgacctttat caatagaaga
     1201 aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttcagcgaca gaccaatcat
     1261 tgctgacgga atgggatcta attcgatacc aaactgtggc agtccgtaat agatcataaa
     1321 cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     1381 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa
     1441 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
     1501 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
     1561 tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg cccttcatct
     1621 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
     1681 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
     1741 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg
     1801 gtcagctcca gttctttcgc aatcgcctgt tggaggttaa ccactttcgg caattgatcg
     1861 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
     1921 ccaacagcca gtccggcgtg agttccggca ctgcccgacg cgaccaccac cgacgaaata
     1981 ttaactgccc cttcacactg ttgcgcaatt tccagcgcac tctccacata acccagcgcg
     2041 cccaaagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
     2101 cgcgtcgcca gctcttccag ttgggtattg ggatcggtca gtgcgtcgca catttcaatc
     2161 tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt
     2221 gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcaactgcg
     2281 gcagtctggc gcacatggtt agactggatc gccccagcag taatcagcgt atcggcacct
     2341 tcacgcagag catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc
     2401 atgggagtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc
     2461 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca gccgtggaaa acgggttaaa
     2521 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
     2581 gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt
     2641 atttggtcac atcagcacca aaccattttt cggaaagggc ttgcagagtg ccgtcttttt
     2701 gcatttccgc aattgcatca ttcactgctt tcagcaggtc ttcatttcct ttacgcaacg
     2761 ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
     2821 tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata cggcctacgc
     2881 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgaca ccctgaacat
     2941 tctgacgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
     3001 gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccag
     3061 aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct
     3121 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttag
     3181 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
     3241 caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa gttccttcca
     3301 gccctaccag cagcgtgccg cgctctttga ctttattaag cagaccttca tctgcaaaac
     3361 ttttaacgct catacccgca accagcgcca cggccatcac acccatcaat gcctgacgtc
     3421 ccagatgtgc taatttcata tttaccccga atgttgttat gtctgtttgc agtgtagagc
     3481 cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag
     3541 aagcaggttg gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt
     3601 gatacttccg taacgcgatg cggtaattgt tggtgctggt ggagggcagc cacggggcga
     3661 ggatttcatc aaggaaaccg tcctgcagct ggtcgaggga aatattttgc tcgtgtagat
     3721 gattccccaa acggcgcaga cgaacgacat attcacgcac cgtaccaggg ctcatttcag
     3781 tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc
     3841 aatgtaaatc accgcaaaat cggcacaatg ccgcccatga ttgttgttcc acttgccagc
     3901 cattttcatc aagcagcgta tccaggcggg aaatctcgat tttattcact attttgccgt
     3961 cgcgaactaa ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt
     4021 gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta
     4081 agaactcctg gtagtcaaaa ttaaagtgcg gcatttactg acgttataac ttacccagtt
     4141 tagtgcgtaa ccgtttaata gcctggctgt gtaactgact gacccgcgat tccccgacct
     4201 ccagcaccgc accaatctct ttgagattca gctcttcctg ataatagaga gttaatacca
     4261 gtttttcgcg ctccggcaac gtttcgatgg cttccatcac ccgctggcgc agattactgt
     4321 ccagcagtta ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat
     4381 cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca
     4441 acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg
     4501 cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc
     4561 gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgta
     4621 tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg
     4681 cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca
     4741 gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg
     4801 agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt
     4861 tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcggg cgattagtgg
     4921 gtgaaatgag gggttatttg ggggttaccg gtaaattgcg ggcagaaaaa aaccccgccg
     4981 ttggcgggga agcacgttgc tgacaaatta cgcttcatgt tgccggatgc ggcgtaacgc
     5041 cttatccggc ctacaaaaat ctgcaaatta aataaattgc aattcacctt gtaggcctga
     5101 taagcgcagc gcatcaggca atttggcgtt gccgccagtc tcagttaatc aggttacgac
     5161 gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt tgccagtacg
     5221 gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc ggtcgcatag
     5281 tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tggtggtgtt gttcaggttg
     5341 gttactgcgg aatccagacg gttttgcacc gcaccgaggg aggagcggaa agtatcaacc
     5401 tgtgcaatag ctttgtctaa aagtgccagt ggatcagcag aagaagcccc tgagaactgt
     5461 gcagtcggtt ttgctgttga taattcaaca acaactttat tatctgttgc ttttgccgca
     5521 gtattatcat caagtgccgt tgaaaccgtt gtagcgtagt ttttaccttg gaatgttaca
     5581 tatcccgcag ccgagccgtc agagtcagcg gcaactttaa tcagttgatc tttctgagta
     5641 gccgcagtcg ttagtccatt atcggtgtct gtgtattcga catcggcttt attcaacgtg
     5701 actttgccgt ctgtatgatt aatcgacgcg gagtagaaat cattgccgga ttgaacgacg
     5761 aactgtgatg tagcagcccc tttcgcatcc agagtgttgt gtaacgtcag gctagaaaca
     5821 tcgactgttt tacctgtcgc agtagtcaga tctttcgcaa cagcagtgaa atcaacacca
     5881 acaggttttg tcccggcggc accggtaatt tgagtaatgg aatcactaac gttaagtgac
     5941 tgacccgaaa cggagaaacc gttcagtcct aatgtagaag agtcaatctt ctgcaaatca
     6001 atgctaatag tctgcccatc attcgcgcca acctgaatct tcatcgaacc atctttcgcc
     6061 agcacattca cgccgttgaa ctgggtctga ccggaaacgc gatcaatttc atccagacgg
     6121 gatttgattt cgtcctgaat agaagacagg tcagaatcag agttagtccc ggtcgtcgcc
     6181 tgaacggtca actcacgcac acgctgcaag ttgttgttga tttcagacag tgcgccttcc
     6241 gtggtctgcg ccagagaaat accgtcgttg gcgttacgtg cggcctgagt cagacctttg
     6301 atgttagaag tgaagcggtt agcaatcgcc tggcccgcag cgtcatcttt agcgctgtta
     6361 atacgcagag cagaagagag gcgctcgata gaagtcgaca gcgcagactg gtttttgttg
     6421 atgttgttct gagtgatcag cgagaggctg ttggtattaa tgacttgtgc cataattcat
     6481 tttcctgttt tcaagtctac ggtttaagta ttgggcttcc acccttcggc ttcgtcgccg
     6541 tcaaccctgt tatcgtctgg tcactcacaa cctttagaat ttttttctag cggtcgaaat
     6601 actccccttc ctcgttgctg ttcgtgccat taaggcaaaa aataaatcgt aaactttacc
     6661 ccgcttacgc cgataacccc ggtattcgtt ctatacacgt cgcatgatta aggaaactat
     6721 atggctagta tttcaacgct tggggttggg tcggggctgg acttaagcag catccttgac
     6781 agtctggagg cggcagaaaa atctaccctg acgccgatat caaaacagca aagctcttac
     6841 accgcgaaac tgagcgccta cgggacgctg aaaagtgcac tagagtgttt ccagacagcc
     6901 aacacagcac tgaataaagc cgatttattt accgccacct caaccaccag cagttcatcg
     6961 gctttcagcg ccaccaccac gggcagcgcg attgctggga aatacaccat tagcgttagc
     7021 caactggcgc aggcacagac gctgacaaca aaaaatagtc aaaaagacag taaagccgcc
     7081 atcgccacca gcgatagcgt actgaccatc caacaaggcg gcggtaaaga tcctgtcacg
     7141 attgatatca gcgcgggtaa ctcgtcactt tccggcattc gcgatgccat taacaacgcc
     7201 aaagctggtg tcagcgccag tatcatcaat gtgggaaacg gcgaatatcg tctgtccatc
     7261 accgctaacg atacgggcag cgataacgct atgaaactta gcgtcagcgg cgacagcgcc
     7321 ctggaaagtt ttatgggtta caacggaacg ccgggtgata gcagcaatgg catgattgaa
     7381 agcgtcaccg cgcaaaacgc caaactgaca gtcaataacg ttgagattga aaacagcagt
     7441 aacactatca gcgactcact ggaagatatc accctcaacc tgaatgacgt caccaccggt
     7501 aatcagacac tgaccatcag taaagacaca tccaaagctg aaaatgcggt taaagcgtgg
     7561 gtggatgcct ataacacatt gcaggataca ttcagcagct taaccaaata caccgctgtc
     7621 gatgccggag ctgaaagtca ggactccagt aatggcgcat tactcggtga ctccacactg
     7681 cgtaccatcc agactcagct taaaaccttg ctcgccaata ctcacagcag ttcaaactat
     7741 aaaacgctgg cacagattgg cattaccagc gatgccagta ccggcaagct ggaaatcgcg
     7801 actgacaaat tgcaaacggc gctaaaaaat gatgccgctg gtatcggtga aatgtttata
     7861 ggtgacggca aaagtactgg ggtaaccacc ggcattagta gcaacctgac cagttggctc
     7921 tcttccaccg ggataatcca ggcagcaaaa gatggcgtca gcaaaacgct gaataatttg
     7981 accgaccagt acaacgcagc cagtgaacgt atcgatacct taatgacgcg ctataaagcc
     8041 cagttcaccc aattagatgt gttaatgaac tcgctgaact ccactagtag ctatctgaca
     8101 cagcagttcg acacctcaaa tagcaactcg aaataatctt caggagagac tatgtacgcg
     8161 gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc
     8221 caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt tagagcgcgc
     8281 ctgtttatgc aggacaacaa tcagcaaggc aaaggtgtct ctttgtcaaa agcgatcaac
     8341 atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga attaacccaa
     8401 aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa tttacgcaac
     8461 gatgtctccg cagtcgaaga ggtggaagca ttaatgcgta atattgccga tgcctggaaa
     8521 gcgtctttac tctccccttc tttgattcag gacccagtct gatgaacaat gcaccgcatt
     8581 tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc ctggcgacag
     8641 aagaacaatg ggacgaactc atcgccagcg aaatggcgta tgtgaatgcg gtgcaggaga
     8701 ttgcacatct gactgaagag attgacccat ccaccacgat gcaggagcag ctccgcccga
     8761 tgctgctcct gattctcgac aacgaaagca aggtaaagca gttattacag attcggatgg
     8821 atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg
     8881 gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc ttaatgagcc
     8941 gatggctcgc gaataatccg attacggcta cgcttctaat attccccttg aatggagtcg
     9001 aagaatgcgt aatcccactc tgttacaatg ttttcactgg tattacccgg aaggcggtaa
     9061 gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta tcaatatggt
     9121 ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct acgactccta
     9181 tgatttattt gatttaggtg agtttgatca gaaaggcagc atccctacta aatatggtga
     9241 taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg cggtgctgct
     9301 ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg
     9361 tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg aaggctggac
     9421 gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg atttcaaatg
     9481 ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta aaattgttaa
     9541 cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta atttcgatta
     9601 tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga ttaaatactg
     9661 ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg cggtcaaaca
     9721 tattccggca tggttttata aagagtggat cgaacacgta caggaagttg cgccaaagcc
     9781 gctgtttatt gtggctgaat actggtcgca tgaagtcgat aaactgcaaa cgtatattga
     9841 tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat tccatgaagc
     9901 atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc tggtggaagc
     9961 cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt tgcaagccct
    10021 cgaagccccc gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt tgttgcggga
    10081 aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattatg aagatgtcgg
    10141 tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg atgagttaat
    10201 tctcgcccgt cagcgtttcg cccacggtgt acagacgtta tttttcgacc atccgaactg
    10261 cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa
    10321 cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata aaacctggcg
    10381 tgattttctg gggaaccggc aagagagcgt agtgaccgac gaaaacggcg aagcaacctt
    10441 cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt aaattcatcc
    10501 ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc gctttatcca
    10561 gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata ccatcgtact
    10621 caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta acgttagtga
    10681 gatcatcaga catcaatgtc aacttacccg gcacggcaat cacccgctgc cactgacggc
    10741 aatcgagggt atcgccctct ttagtgacaa tgaggctgcc tatcgcttcc ggactgacca
    10801 acgcacgttg cggccctttg gtctgccagt aacccgtcag ccagtccggc gcgggggttt
    10861 tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg agcgcacctg
    10921 caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt gtggcattaa
    10981 agccctgatg tcgccagcct ttccaggctc agagagaaat ttgatctaac cattattttt
    11041 gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcgactc ttgtttcaaa
    11101 ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg cctggttgat
    11161 taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca cttactattt
    11221 tggcatcact ggcacatttt gggcagtcac gggtgaattt acccgttggg gcggtcagct
    11281 tttgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta tccatctgga
    11341 aggatcgcca ttaacccgca tcgacggaat gatgatcctc ggtatgtttg gcggctgttt
    11401 tgccgcagcg ctgtgggcca acaatgtcaa actgcgcatg ccgcgcagcc gtatccgcat
    11461 tatgcaggcc atcattggcg gtattatcgc cggttttggc gcacgtctgg caatgggctg
    11521 taacctggcg gcgttcttta ccggaattcc acagttctcg ctgcacgcct ggttctttgc
    11581 catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc ccatcttccg
    11641 tattcccgtg aaaatgcaga aagtttctgc tgcctcaccg ttgacgcaaa aaccggatca
    11701 ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg gctgggcgct
    11761 gctgacggcg atgaaccagc ccaaactggg gctggcaatg ctgtttggcg tcggctttgg
    11821 tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata tgtggatcac
    11881 cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtaagtg ccatcgggat
    11941 cttcagttac gtacagttag gcgttgaacc caaaatcatg tgggcgggac caaacgcggt
    12001 aattggtggt ttactgtttg gttttggcat cgtgctggca ggcggctgcg aaaccggctg
    12061 gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg gcaacgtgat
    12121 cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg ccaccgactg
    12181 ggacaaaatc aacctcctga aaacctttgg cccgatgggc ggcctgctgg tgacatattt
    12241 gctgttgttt gcagctctaa tgttgattat cggctgggaa aaacgcttct tccgccgtgc
    12301 ggcaccgcag attgctaagg agatcgcatg aaaaatatcg ttcctgatta ccgtcttgat
    12361 atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat gccgcagtta
    12421 aaaaaagggg agatcctgga agtggtgagc gactgtccgc agtcgatcaa taatattcca
    12481 ctggatgcgc gtaatcacgg ctatacggtg ctggatattc agcaagacgg gccgaccatt
    12541 cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg gaacatcatt
    12601 acctctctat cacatagatt ttacccgccc cctctcctat tcttttcagt gtgacctcag
    12661 gaggtttcta tgtgtggacg ctttgctcaa tcccaaacgc gtgaagatta ccttgcgctt
    12721 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacctattgg cagatacaac
    12781 gtcgcgccgg gaaccaaagt ccttctgctc agtgaacgtg atgaacacct tcatctggat
    12841 ccggttttct ggggatatgc tcccggaggg tgggataaac cgccgctgat taacgcccgc
    12901 gtagaaactg ccgcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    12961 tgctttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaca gccttatttt
    13021 atctatcgcg cagatggaca acctgttttt atagccgcga taggtagcac accgtttgag
    13081 cgcggtgacg aagccgaagg atttttaatt gtcactgcag cagcagacca gggtctggta
    13141 gatattcatg accgccgccc gctggtactg tcgccagaag ctgcacggga atggatgcgg
    13201 caagagatta gcggtaaaga agcctctgaa attgctgcca gtggctgtgt tccagcaaac
    13261 cagttcacct ggcacccggt atcgtgcgcg gtgggtaatg ttaaaaacca gggcgcggag
    13321 ttaattcaac ctgtttgata agcctggaga ttatctacac ctggatggac atcattgtac
    13381 tatgccccgt acatgcccca tcacatcctc aacatcaaag tattagagcc ttgcaaacca
    13441 ttatgcgtct cagtttgtct catctatctt acgcaaaaaa aaatagaac
//
LOCUS       DAZFRG010000127        13476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFRG010000127 DAZFRG010000000
VERSION     DAZFRG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.127"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            100..1767
                     /gene="fdrA"
                     /locus_tag="ACHKUC_002998"
     CDS             100..1767
                     /gene="fdrA"
                     /locus_tag="ACHKUC_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6619220.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1777..3036
                     /gene="ylbE"
                     /locus_tag="ACHKUC_002999"
     CDS             1777..3036
                     /gene="ylbE"
                     /locus_tag="ACHKUC_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6619221.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQYIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDVGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3047..3862
                     /gene="ylbF"
                     /locus_tag="ACHKUC_003000"
     CDS             3047..3862
                     /gene="ylbF"
                     /locus_tag="ACHKUC_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6619222.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALATSDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3859..4752
                     /gene="ybcF"
                     /locus_tag="ACHKUC_003001"
     CDS             3859..4752
                     /gene="ybcF"
                     /locus_tag="ACHKUC_003001"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6619223.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(5085..6152)
                     /gene="purK"
                     /locus_tag="ACHKUC_003002"
     CDS             complement(5085..6152)
                     /gene="purK"
                     /locus_tag="ACHKUC_003002"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO6619224.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6149..6658)
                     /gene="purE"
                     /locus_tag="ACHKUC_003003"
     CDS             complement(6149..6658)
                     /gene="purE"
                     /locus_tag="ACHKUC_003003"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO6619225.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(6754..7083)
                     /locus_tag="ACHKUC_003004"
     CDS             complement(6754..7083)
                     /locus_tag="ACHKUC_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619226.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFANSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(7194..7916)
                     /gene="lpxH"
                     /locus_tag="ACHKUC_003005"
     CDS             complement(7194..7916)
                     /gene="lpxH"
                     /locus_tag="ACHKUC_003005"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO6619227.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQTAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(7919..8413)
                     /gene="ppiB"
                     /locus_tag="ACHKUC_003006"
     CDS             complement(7919..8413)
                     /gene="ppiB"
                     /locus_tag="ACHKUC_003006"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO6619228.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8587..9972
                     /gene="cysS"
                     /locus_tag="ACHKUC_003007"
     CDS             8587..9972
                     /gene="cysS"
                     /locus_tag="ACHKUC_003007"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO6619229.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(10008..10529)
                     /gene="ybcI"
                     /locus_tag="ACHKUC_003008"
     CDS             complement(10008..10529)
                     /gene="ybcI"
                     /locus_tag="ACHKUC_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6619230.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(10637..10849)
                     /gene="ybcJ"
                     /locus_tag="ACHKUC_003009"
     CDS             complement(10637..10849)
                     /gene="ybcJ"
                     /locus_tag="ACHKUC_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO6619231.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(10851..11717)
                     /gene="folD"
                     /locus_tag="ACHKUC_003010"
     CDS             complement(10851..11717)
                     /gene="folD"
                     /locus_tag="ACHKUC_003010"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO6619232.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            11988..12064
                     /locus_tag="ACHKUC_003011"
     tRNA            11988..12064
                     /locus_tag="ACHKUC_003011"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12022..12024,aa:Arg,seq:tct)
     gene            complement(12080..12681)
                     /locus_tag="ACHKUC_003012"
                     /pseudo
     CDS             complement(12080..12681)
                     /locus_tag="ACHKUC_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            12688..13062
                     /locus_tag="ACHKUC_003013"
                     /pseudo
     CDS             12688..13062
                     /locus_tag="ACHKUC_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(13117..>13476)
                     /locus_tag="ACHKUC_003014"
     CDS             complement(13117..>13476)
                     /locus_tag="ACHKUC_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6619233.1"
                     /translation="SHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQ
                     ALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENV
                     KTKVKGTVVMFSASGKK"
BASE COUNT         3120 a         3490 c         3741 g         3125 t
ORIGIN      
        1 gatattcatc accttattta ctcgttgtca tcgataccgt aatcgccaca ttaacactgc
       61 tcgtgcaatt gccatgggtg caatttttaa ggagttgtta tgatccacgc ctttattaaa
      121 aaagggtgtt ttcaggattc ggtcagttta atgattattt cacgaaaact cagcgaatca
      181 gaaaatgttg atgatgtttc cgtaatgatg ggtacgcccg ccaataaagc gttattagat
      241 actacaggtt tctggcatga cgattttaat aacgccacgc cgaacgatat ttgcgtggca
      301 attcgtagcg aagcggcgga tgcggggatc gcgcaggcgg ttatgcagca gcttgaagag
      361 gcgctaaaac aactggcgca gggttcaggc agcagccagg cgttgacgca ggtgcgtcgc
      421 tgggacagtg cctgtcagaa attacccgat gccaatctgg cgctgatttc agtggctggt
      481 gagtatgcgg cggagctggc gaaccaggcg ctggatcgca acctcaacgt gatgatgttt
      541 tccgataacg tcacgctgga agatgaaatc caacttaaaa cccgcgcgcg ggaaaaaggc
      601 ttgctggtga tggggccaga ctgcggtacg tcgatgattg ccgccacgcc gctggctttt
      661 gctaacgtga tgccggaagg caacattggc gtcataggcg cttccggtac cgggattcag
      721 gagctgtgct cacagattgc gctggcaggg gagggaatta ctcacgcgat tggtcttggc
      781 gggcgcgatc tcagccgtga agtgggcggc atcagtgcgc taacggcgct ggaaatgctc
      841 agtgcagacg agaaaagcga agtgctggca tttgtttcaa aaccacctgc cgaaactgtg
      901 cgtctgaaaa ttgttaatgc catgaaagca actggcaaac cgacggtggc gctgttttta
      961 ggttataccc cggcggtggc ccgcgacgag aatgtctggt ttgcctcctc gctggatgag
     1021 gccgctcgtc tggcttgtct gctttcacgc gtcactgcgc gacgtaacgc gatagcgcct
     1081 gtcagcagcg gatttatttg cggtttgtat accggcggta cgctggctgc tgaagcggcg
     1141 ggattacttg ccggacacct tggcgtggaa gccgacgata cccatcaaca tggcatgatg
     1201 ctggacgccg acggacatca gatcctcgac ctgggcgatg atttttacac cgtcgggcgt
     1261 ccccatccga tgatcgaccc gaccttacgc aacctgttaa ttgccgatct cggcgctaaa
     1321 ccgcaagtgc gcgtgttgct gcttgatgtc gtgattggct tcggtgcgac cgccgatcct
     1381 gccgcctcgc tggtgagcgc ctggcaaaaa gcctgtgccg cgcgttcaga caatcaacca
     1441 ctgtatgcca ttgccacggt gacaggcact gaacgtgacc cgcaatgccg ctcgcagcaa
     1501 atcgccacgc tggaagatgc ggggatcgcg gtagtgagtt cgctaccgga agccaccttg
     1561 ctggctgcgg cgttaattca tccgctctcg cctgccacac agcaacacac accgtcatta
     1621 ctggaaaacg tcgccgtgat taacatcgga ttacgcagct ttgcgctgga gctacaaagc
     1681 gccagcaaac cggttgtgca ttaccaatgg tcgccagtcg ccggcggcaa taaaaaactg
     1741 gctcgtttat tagaacgttt gcaataaggg gttcccatgt ttacatcagt ggcgcaagcc
     1801 aatgctgcgg ttatcgaaca aattcgtcgc gctcgtccac actggctgga tgtgcaaccg
     1861 gcttcttcac ttatcagcga actaaacaag ggcaaaacac tgcttcacgc cgggccgcca
     1921 atgcgctggc aggagatgac cggacccatg aaaggggcat gtgtgggcgc atgtctgttt
     1981 gaaggctggg cgaaagatga agcgcaggca ctggcaatgc tggagcaggg ggaagtgaac
     2041 ttcattcctt gtcaccatgt gaatgctgtc gggccaatgg gcggcattac ttctgccagt
     2101 atgccgatgc tggtggtcga gaacgtgacc gacggcaacc gggcgtactg caacctcaac
     2161 gaaggtatcg gcaaagtgat gcgttttggc gcttacggcg aagatgtcct gactcgccat
     2221 cgctggatgc gcgatgtgtt aatgccagta ttaagtgcgg cgctggggcg catggagcgc
     2281 ggtatcgatc tcaccgcgat gatggcgcag ggcattacca tgggcgatga gttccatcaa
     2341 cgcaatattg cttcctctgc actgttaatg cgtgcgctgg ccccacaaat tgctcgcctc
     2401 gatcatgata aacagtacat tgcggaagtg atggatttcc tcagcgtgac cgatcagttc
     2461 ttcctcaacc tcgcgatggc ctactgcaag gcggcgatgg atgtaggcgc gatgatccgc
     2521 gcaggcagca tcgttacggc tatgacccgc aacggcaata tgttcgggat tcgggtaagc
     2581 gggctgggtg aacgctggtt tactgcgcct gtaaacactc cgcaaggtct gtttttcacc
     2641 ggcttctcgc aggagcaggc gaacccggat atgggcgata gcgcgattac cgaaaccttt
     2701 ggtatcggag gtgcggcaat gatcgccgca cctggcgtaa cacgctttgt cggtgcgggt
     2761 ggcatggaag cggccagagc ggtatctgaa gagatggcgg aaatttacct tgaacgcaat
     2821 atgcagttgc agatccccgg ctgggatttc cagggggcgt gcctggggct ggacattcgt
     2881 cgcgtggtag aaaccggcat tacgccactc atcaataccg gtatcgccca taaagaggcg
     2941 gggatcgggc agattggcgc aggcaccgtg cgggcaccgc tggcgtgctt tgaacaggcg
     3001 ctggaagcac tggctgaaag catggggatt ggttgaggaa cgcacaatga cgatcatcca
     3061 tcctctactt gccagccgta gcgcaccgaa ttatcgccag tcctggcggt tagcgggagt
     3121 gtggcggcgg gcgattaacc tgatgacgga aagcggcgaa ctgttaacgc tgcatcgtca
     3181 ggggagtggt ttcggccccg gcggatgggt gcttcgccgc gcgcaattcg atgcgttatg
     3241 cggtggatta tgcggcaatg aacgaccaca ggttgtggca caagggattc gtctcgggcg
     3301 tttcacggtt aaacagccac agcgttattg tttgctacgt attacgccgc ctgcgcatcc
     3361 tcaaccgctt gccgttgcat ggatgcaacg cgcggaggaa accgggcttt tcgggccact
     3421 ggcattggcg acaagcgatc cgctgcctgc tgaattacgc cagtttcgtc actgttttca
     3481 ggccgcgctc aatggcgtta agaccgactg gcggcactgg ctgggtaaag gccccggatt
     3541 aacgccaagt catgatgaca cgctgagcgg aatgctgctg gcggcctggt attatggggc
     3601 tttagatgcg cgctccggtc gcccgttttt tgcctgttcc gacaatcttc aactcgttac
     3661 cactgcggtg agcgtcagtt atttacgtta tgccgcgcaa ggatatttcg cctcgccact
     3721 cctgcacttt gttcatgctc tgagttgccc gaaacgtacc gcagcggcga tcgattcgct
     3781 gctggcgctg gggcatacgt caggggccga tacgctgctg gggttctggc ttggccaaca
     3841 attattacaa gggaaaccat gaaaacactg gttgtggctc ttgggggcaa cgccttactc
     3901 cagcgcggtg aggcgctgac ggcaaaaaat caatatcgca atattgccag tgctgtaccc
     3961 gcgctggcgc gcctggcccg ttcttatcgg ctggcgattg ttcacggcaa cggaccgcag
     4021 gtggggctgc tggcgttaca gaatctggcg tggaaagagg tagaaccgta tccgctggat
     4081 gtactggtgg cggaaagcca ggggatgatt ggctatatgc tggcgcagag cttgagcgca
     4141 cagccgcaga tgccgcctgt cacgacggtg ctgacgcgca ttgaggtttc gcctgatgat
     4201 ccggcgtttt tacagccaga gaaatttatt ggtccggttt atcagccaga agaacaagag
     4261 gcactggaag cggcttacgg ctggcagatg aaacgtgatg gtaaatattt acgccgggtg
     4321 gtggcgtctc cgcaaccgcg taaaattctc gacagcgaag ccatcgagtt gttgctcaaa
     4381 gaggggcatg tggtgatttg cagtggcggc ggcggtgtgc ctgtgacgga agacggggca
     4441 gggagtgaag cggtgattga taaagatctc gccgctgcgt tgctcgccga gcagattaat
     4501 gccgatggac tggtgatcct caccgatgct gatgcggtat atgaaaactg gggaacgccg
     4561 cagcaacgtg ccattcgcca tgccacaccg gatgagttag cgccatttgc caaagccgat
     4621 ggttcgatgg ggccgaaggt aacggcggtg agtggctatg tcagaagccg tggtaaaccc
     4681 gcgtggattg gggcgttatc gcggattgaa gagacgctgg cgggcgaagc ggggacttgt
     4741 atttcgctgt agtgatgggc accaggcaat tggcacatcc agcctgatgc gatgctggcg
     4801 catcttatca ggcctacagg cggtgtcgga acggtaggcc ggataaggcg tttacgccgc
     4861 atccggcaat cggtacacca tgcctgatgc gatgctggcg catcttatca ggcctacagt
     4921 cggtgttgga gcggtgggcc ggataaggcg tttacgccgc atccggcaat cggcacacca
     4981 tgcctgatgc gatgctggcg catcttatca ggcctacagt cggtgttgga acggtaggcc
     5041 tgataaggcg ttcacgccgc atgcggcaag aaaatgagca tcaattaact gaacttgctc
     5101 tgcgcccaca tcacgccgct ggcatactcc ggcggcagca gtgggattaa ggcttccagc
     5161 gtcgcggtca gacgcgatgt gtcgctgtcg gtcaaattca aatgccccac tttacggccc
     5221 ggacggactt ctttgtcgta ccagtgcaga tgcacaagcg gcagtttcag ccagtcataa
     5281 ttcagatcgc taccaatcag attgatcatc accgacggat tattcactac tggttgcggt
     5341 aacggcagat cggtaattgc ccgcagatgt agctcaaact ggctgatgct ggcaccgttt
     5401 tgcgtccagt gaccgctgtt atgcacacgc ggagccagtt cgttgatcag cagaccttgt
     5461 ggggtgacaa aacactccat tgccatcacg cctacatagc tcagctcctg cataatcgcc
     5521 gacaacatct cttcagcttg cgcctgcagc tgtgcgttgg cctgcgggaa agcgacgctg
     5581 gtgcgcaaaa tgccgtcctg atgcaggtta tgcgtcagcg gataaaacac ggtgctgcca
     5641 tcaaaaccgc gcgcgccaac cagcgacact tcaccagaga agtttatgcc ctgctcgaca
     5701 atacattcgc cgtagcattc agcaggtaac tgttcggttt catcggcgcg taaacgccat
     5761 tgaccgcgtc cgtcatagcc accagtgcgg cgcttaacaa tcgccagctc acctaaacga
     5821 tcaaacaccg caggccactc gctgcgttcg gaaagtaact gccacggtgc ggtcggcagg
     5881 tggagcttat cgaaaagctg cttctgagtc agacggtcgg caataatcgg gaacacatcg
     5941 cggttcacaa aggccggatg gcgcgccagc tcgcgggtta atgcagtttc cggccagcgt
     6001 tcgatctcag cggtaatcac gctttgttga aaaggcaccg ccgccggttc agcgtccagc
     6061 ccgaccggcc agacagcaat gcctaacggt tcgcctgcct gacgcagcat acggcctaac
     6121 tgcccgttac cgaggacgca aacctgtttc atgccgcacc tcgcgggtcc gggttttcca
     6181 gcacttcatc agtttgtgct ttgcgccatt cattcagacg ctggtgcagt tctttatcat
     6241 gagtcgcaag aatttgtgct gccagtaacg ccgcgtttgc cgcgccagct ttaccaatcg
     6301 ccagcgtacc caccggaatg ccgcgcggca tttgtacgat ggagtagagg ctatcgacac
     6361 cgctcagtgc ggcgctctgt actggcacgc ccagcaccgg caccagcgtt ttggcggcaa
     6421 tcatgccagg caggtgcgct gcgccgcctg cgcccgcaat aatcacctga taaccgttct
     6481 cttcggcgct ttcggcgaag ctgaacagtt tatcgggggt gcggtgagca gaaaccactt
     6541 caacgtggtg cgggacattc aggatttcga agatttcggc ggcgaactgc atggtagccc
     6601 agtcgctttt ggaccccatc acgatggcga cacgcgccgg attattgcgg gaagacatgc
     6661 gtcttaaaac tcctgtggtg cacaactctc ggctttagag ggcacagaga atagcacgga
     6721 aagagtgcaa ggaaaacggt tgcgtaggcg gaattaaccg tcagttgttg agtttttaat
     6781 ttgttctgcg tgttgattat gcgctctggc tgaattggca aagaatgccc atatcaacac
     6841 aatgctccat ggaaaaaagc aaataccgac aatcaatata aatctttcta caaaacgttg
     6901 gttatggggg cgggtgagta ctaaataaat gagccagaaa tagactatcc aggaaatgac
     6961 cgccgcagct accagtgaca atgcgccaaa atagaagccg acaagaccga accagctaat
     7021 acacagtggc gctcctgccg taagggcgat gccagaaatc aaaaatccgt tgcagcgttg
     7081 cataataata ttccctgttg ttatttcact cagagattat taccttgctg attttaaaaa
     7141 agatatacag gttgcgttag ttcggtgctt tttcagaatc tgtccattcg tggtcagaaa
     7201 ggaaaatgga ttagctcaac gtcatccgcc gtgactttca ccatcgaacc ttctgtatgc
     7261 caggcaccca gtaccacgcg aaaagccgtt tgctgattgg cgataagttc atgcaccgcc
     7321 gggcgatggg tatgcccgtg gatcagccat tgcacttgat gtttttccat cgcactgacc
     7381 accgcgtttt ggttaacgtc catgatcgcc agcgatttac tgctgttggc ttctttactg
     7441 ttcgcgcgca ttcgcgcggc aatgcgtttg cgcacaaaca acggcagggc gaggaatagc
     7501 gtttgcagcc agggtttgtg gactttggca cgaaaagcct gataacccgc gtcatcggtg
     7561 cacagcgtgt cgccatgcat aatcaacacc cgacgaccat aaagttcgag caccttttct
     7621 tccggcaata acgtcatgcc gctttcacgg gcaaagcgtt tgccgagcag aaaatcacgg
     7681 ttgccatgaa tgaaataaca gggaacgccg gaatcggata ccgctttgat cgctgcggcc
     7741 atctggtgat gaagtgggtt gggatcgtcg tcgccgatcc aggcttcaaa cagatcgcca
     7801 agaatataca gcgcatcggc cttgcgggcc tcccccgcta aaaaacgcag aaaaccggcg
     7861 gtgatcgccg gttcttccgc gcagagatga agatctgcaa taaagagtgt cgccacgatt
     7921 actcgctaac ggtcacgctt tcaatgataa cgtcttcttt tggcacgtcc tggtgcatac
     7981 cgctacgacc ggttgccaca cctttgattt tgtctaccac gtccatgcct tcaaccactt
     8041 cagcaaatac gcagtagccc caaccttgca ggctttcgcc agagaagttc aggaagtcgt
     8101 tatcaaccac gttgatgaag aactgggcag ttgcagagtg cggtgcctga gtacgggcca
     8161 ttgccagcgt accacgggta tttttcagac cgttgttggc ttcgtttttg atcggttctt
     8221 tggtggcttt ttgtttcatg cccggttcaa aaccgccgcc ctgaatcata aagccgttga
     8281 taacacggtg gaaaatggtg ttgttgtaaa aaccttcgcg gcagtagtcc aggaagtttt
     8341 taactgtttc aggtgcttta tcgtcaaaag ttttgatgac aatatcgccg tgattggtgt
     8401 ggaaagtaac catttttgca tcctgttccg tttgattggt gcttcaaccc ggttcgggtc
     8461 atatataggg tggtgttata gcataaccgc acgatcggat catcacgcaa tgtatgctga
     8521 ttcgcgcggg aaatatgggt attatacgca actcaattac ccacacatgt ctaaacggaa
     8581 tcttcgatgc taaaaatctt caatactctg acacgccaaa aagaggaatt taagcctatt
     8641 cacgccgggg aagtcggcat gtacgtgtgt ggaatcaccg tttacgatct ctgtcatatc
     8701 ggtcacgggc gtacttttgt tgcttttgac gtggttgcgc gctatctgcg tttcctcggc
     8761 tataagctga agtatgtgcg caacattacc gatatcgacg acaaaatcat caaacgcgcc
     8821 aatgaaaatg gcgaaagctt tgtggcgctg gtggatcgca tgatcgccga aatgcacaaa
     8881 gattttgatg ctttgaacat tctgcgcccg gatatggagc cgcgcgcgac gcaccatatc
     8941 gccgaaatta ttgaactcac tgaacaactg atcgctaaag gtcacgctta tgtggcggac
     9001 aacggcgacg tgatgttcga cgtcccgacc gatccgacat atggcgtgct gtcgcgtcag
     9061 gatctcgatc agctgcaggc aggcgcgcgc gttgacgtgg tcgacgacaa acgcaacccg
     9121 atggacttcg ttctgtggaa gatgtcgaaa gagggcgaac cgagctggcc gtctccgtgg
     9181 ggcgcgggcc gtcctggctg gcacattgag tgttcggcaa tgaactgcaa acagttgggt
     9241 aaccactttg atatccacgg cggcggttct gacctgatgt tcccgcacca cgagaacgaa
     9301 attgcccagt ccacctgtgc gcacgatggc cagtatgtga attactggat gcactccggt
     9361 atggtgatgg ttgaccgcga gaagatgtct aaatcgctgg gtaacttctt caccgtgcgt
     9421 gacgtgctga aatactacga tgcggaaact gtgcgttact tcctgatgtc gggtcactat
     9481 cgcagccagc tgaactacag cgaagagaac ctgaaacagg cgcgtgcggc gctggaacgt
     9541 ctctatactg ccttgcgcgg cacagacaaa accgttgcgc ctgccggtgg cgaagcgttt
     9601 gaagcgcgct ttattgaagc aatggacgac gatttcaaca ccccggaagc ctattccgtg
     9661 ctgtttgata tggcgcgtga agtaaaccgt ctgaaagcag aagatatggc agcggcgaat
     9721 gcaatggcgt ctcacctgcg taaactttct gccgtattgg gcctgctgga gcaagaaccg
     9781 gaagcgttcc tgcaaagcgg tgcgcaggca gacgacagcg aagtggctga gattgaagcg
     9841 ttaattcaac agcgtttgga tgcccgtaaa gcgaaagact gggcggcggc agatgcggcg
     9901 cgtgaccgcc ttaacgagat ggggatcgtg ctggaagatg gcccgcaagg gaccacctgg
     9961 cgtcgtaagt aattgcgcta ttgccggatg cgagttttcg catccggtta tcgtctgcgc
    10021 caccacaaca ttcccatcag tagcatcccc ggcaaccaca cccacatcaa ttcagaaata
    10081 atcacctgat gcccgtacgg cgtggtgtaa cgagacaatg caaacggcgc gactttaatc
    10141 acctgccagg gagcgaaaaa gcgttcatct gaccacggcc acagccagcc aaccccttta
    10201 ccgccagtgg ttaccgaatc cagcaagctg tgcgatagca acgagacggt taaaaacagc
    10261 cagcagcgaa tcagcccagc cctgaaccat cgacgtccaa tcagcacaca taacagcggg
    10321 acaacaaacg caaacaccag cgaatgggta aacccgcgat gaccaaaaac attgccgtaa
    10381 gcaacgccaa atttaaacga caatacatca gcatcgggca gcatcgccag gatgattccg
    10441 gcaaataaca gacgcggagg gatggctttc gaacccaacc ctaaaccaat gcatagggga
    10501 acggcggcgt gcgtaataac ggttggcatg atggtcgctt cggcaaaatg tcgatgctat
    10561 cagcatggat gaacggggcg tagagggcaa aagtctgaaa agagaaccgg cctgttgata
    10621 caggccggga aagggatcag gcaacaacct gtacgctgtg acctgcaaaa ctcactgtct
    10681 gaccggcgac gattttgcag cgtttgcgcg tttcaaccgc accgtcgact ttcacctggc
    10741 cttcggcaat cgcgattttc gcctgcgcgc cgctttcgct ccagccttcc agtttcagca
    10801 agtcgcacag ctcaacgtgc ggatgtttac ctaaagaaaa tgtcgccatg ttactcatcc
    10861 tgtggatcat gatattcaac gcacgcctgt agcgtgtttt caatcagcgt ggcaaccgtc
    10921 atcgggccaa cgccgcccgg aacaggcgta atgtatgagg cgcgtttagc cgcgtcttca
    10981 aacacgacgt cgcccacaac tttgccattt tccagacggt tgatgccaac atcaatcaca
    11041 attgcgcctt ctttgatcca gtcaccagga ataaagcccg gcttgccgac ggcaacgatc
    11101 agtagatcgg cattttctac gtggtgacgc agatttttag tgaaacggtg agtcacggtg
    11161 gtggtgcaac ctgccagcag cagttccatg ctcattgggc gaccgacgat attcgatgcg
    11221 ccaatcacca cggcgttaag gccgaaggta tcaatgttgt aacgctcaag cagcgtgacg
    11281 ataccgcgcg gggtacaggg acgcagacgt ggcgcgcgct ggcacagacg accgacgttg
    11341 taaggatgga aaccgtccac gtctttgtcc ggatgaatac gttccagcac tttgacgtta
    11401 tcaatacccg ccggtaacgg cagttgaacc agaatgccat caatggtgtt gtcggcattc
    11461 agcgcatcga taagctccag cagctccgct tcgctggtgg tttccgggag gtcataagag
    11521 cgggagacga acccgacttc ttcacaagcc ttgcgtttgc ttgcgacata aatttgcgat
    11581 gcagggttgc tacccaccag cacaacggcc agtcctggcg cccgcagtcc ggctgcaata
    11641 cgcgcctgaa ctttttgagc aacttcagag cgcacctgct gcgcaatcgt tttaccgtca
    11701 ataatctttg ctgccatcag agagaggatt ccatctgtta cgtaggtcga aggggatgcg
    11761 tctattttgt cagaagcgtg gcgcgctgtc agttttcgtt ttctgaaaag taaaaccttc
    11821 tgtacctggc tgattcacac ctatcatgat aaccgtcgtt ggtttgccgc catagtgagc
    11881 tacatcagat gcggtcgtac acttgttcag caactggttc aaaagccatt gactcagcaa
    11941 gggttgaccg tataattcac gcgattacag cacaccgtgg tatcaatgcg cccttagctc
    12001 agttggatag agcaacgacc ttctaagtcg tgggccgcag gttcgaatcc tgcagggcgc
    12061 gccattaaaa ttcaatcagt tacgccttct ttatttcttc tataattcca gagtgggaca
    12121 ttatcaccaa aaatgtcgtc tattttcctc gcgtgctcag tcaaatgatt aggcgcaagg
    12181 tgagcatatc tacgaaccat ttctctggac tcccatccgc ccatttcctg aagcactgat
    12241 aatgggacgc ctgacggaat caaccagctt gccctggtat gtctgaggtc atggaaacgg
    12301 aaattttcaa ttcctgcacg acgacaagct gatagccatg ctgtcttgct gtcaatgcgc
    12361 atcttcctga gcgcaggcat tgatgttcca tctgctcgct tagccgccct ggtatgtaca
    12421 cccacatttg tgatgcttgc ctgtttgatc acgcaacact ttagaggcgg tatcgttcag
    12481 cgccacacca atggcgcagt ttgatttgct atcttctgga ttcaccctgg caacttgtcg
    12541 ctgcatgtca atctgttgcc actccagatt tatgatgttc gactttctca gaacagttac
    12601 cagcgcaaat ttgacgacag atttcagtgg tttggggctc tcatcaatca ggcgtttttc
    12661 ctgattcttt tgtaagtctc ttgtgccatt ccacctcata caggtttgcc tgcaaacagt
    12721 actgatattg cactgccaga tattccggct ggcttcgtgg ttgttttcaa cagtgatgag
    12781 gcatcgtggc atctcgttga agaccatcgg ggtaaaacgg tttatgacgt ggcatcaggg
    12841 gacgcgttat ttatttctga gctcggtccg ttaccggaaa atgttacctg gttatccccg
    12901 gcaggggagt ttcagaagtg gaacggcaca tcatggataa aggatacgga ggaagaaacc
    12961 tcgttgctgg aagcctgtaa gatgtatcgg gtgttgctga accgagttga tacatcaact
    13021 gcccctgata ttgagtggcc tgtgaaccct gtcagagagt aatcattggg attatgccgc
    13081 agcacgtcgt gcgcaagaac gtgctgcggc tggatgctat tttttccctg aagcggaaaa
    13141 cattactaca gtacctttaa ccttggtttt aacattctcg aaatgctctg agagtatatg
    13201 tgttaagcct tcttcggaat cttttgtgtt tgaaaagatg cctttctgat tgtaaatgcg
    13261 catcagtttt tgaccgaagc tattgtgcac aacgccgtca ccaagaattg tggctccgta
    13321 tagagttcca tcgtcagtta aggcctgcgc cgcattgcgt attacacagc tttttgtaga
    13381 tatatttcca ggcaggcagt gaagaaggta aaacatggaa atggaatcaa attgaccatg
    13441 taacgccgcg ggataaggat caaaaacatc atggct
//
LOCUS       DAZFRG010000128        13438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFRG010000128 DAZFRG010000000
VERSION     DAZFRG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.128"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            201..1349
                     /gene="cfa"
                     /locus_tag="ACHKUC_003015"
     CDS             201..1349
                     /gene="cfa"
                     /locus_tag="ACHKUC_003015"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO6619234.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(1389..2030)
                     /gene="ribE"
                     /locus_tag="ACHKUC_003016"
     CDS             complement(1389..2030)
                     /gene="ribE"
                     /locus_tag="ACHKUC_003016"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO6619235.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTQA"
     gene            2245..3618
                     /gene="mdtK"
                     /locus_tag="ACHKUC_003017"
     CDS             2245..3618
                     /gene="mdtK"
                     /locus_tag="ACHKUC_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO6619236.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMQNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPVVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(3659..4915)
                     /locus_tag="ACHKUC_003018"
     CDS             complement(3659..4915)
                     /locus_tag="ACHKUC_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6619237.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            5224..5300
                     /locus_tag="ACHKUC_003019"
     tRNA            5224..5300
                     /locus_tag="ACHKUC_003019"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5258..5260,aa:Val,seq:gac)
     gene            5305..5381
                     /locus_tag="ACHKUC_003020"
     tRNA            5305..5381
                     /locus_tag="ACHKUC_003020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5339..5341,aa:Val,seq:gac)
     gene            5490..5795
                     /locus_tag="ACHKUC_003021"
     CDS             5490..5795
                     /locus_tag="ACHKUC_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO6619238.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            5921..7525
                     /locus_tag="ACHKUC_003022"
     CDS             5921..7525
                     /locus_tag="ACHKUC_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO6619239.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKRENHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7537..8349)
                     /gene="ydhT"
                     /locus_tag="ACHKUC_003023"
     CDS             complement(7537..8349)
                     /gene="ydhT"
                     /locus_tag="ACHKUC_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO6619240.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANCGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(8353..9138)
                     /gene="phsC"
                     /locus_tag="ACHKUC_003024"
     CDS             complement(8353..9138)
                     /gene="phsC"
                     /locus_tag="ACHKUC_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO6619241.1"
                     /translation="MNLSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(9135..9803)
                     /locus_tag="ACHKUC_003025"
     CDS             complement(9135..9803)
                     /locus_tag="ACHKUC_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6619242.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTAASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(9867..10505)
                     /locus_tag="ACHKUC_003026"
     CDS             complement(9867..10505)
                     /locus_tag="ACHKUC_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO6619243.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(10518..12620)
                     /locus_tag="ACHKUC_003027"
     CDS             complement(10518..12620)
                     /locus_tag="ACHKUC_003027"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO6619244.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYTIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLVAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQNPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(12641..13267)
                     /locus_tag="ACHKUC_003028"
     CDS             complement(12641..13267)
                     /locus_tag="ACHKUC_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO6619245.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKNITV"
BASE COUNT         3321 a         3460 c         3100 g         3557 t
ORIGIN      
        1 ctacgataaa tattatgtta ttacggggac aggatcgttc ccgactcact atggatggtc
       61 atttcggcaa gggttcctcc tttccctctg ttctacgtcg gattatagac tcgcggtttt
      121 ttctgcgaga tttctcacaa agcccaaaaa gcgtctacgc tgttttaagg ttctgatcac
      181 cgaccagtga tggagaaact atgagttcat cgtgtataga agaagtcagt gtaccggatg
      241 acaactggta ccgtatcgcc aacgaattac ttagccgtgc cggtatagcc attaacggtt
      301 ctgccccggc ggatattcgt gtcaaaaacc ccgatttttt taaacgcgtc ctgcaggaag
      361 gatcgttggg gttaggcgaa agttatatgg atggctggtg ggaatgtgac cgactggata
      421 tgttttttag caaagtctta cgcgcaggtc tcgagaacca actcccccat catttcaaag
      481 acacgctgcg tattgccggc gctcgtctct tcaatctgca gagtaaaaaa cgtgcctgga
      541 tagtcggcaa agagcattac gatttgggta atgacttgtt cagccgcatg cttgatccct
      601 tcatgcaata ttcctgtgct tactggaaag atgccgataa tctggaatct gcccagcagg
      661 cgaagctcaa aatgatttgt gaaaaattgc agttaaaacc agggatgcgc gtactggata
      721 ttggctgtgg ctggggtgga ctggcgcact acatggcgtc taattatgac gtaagcgtgg
      781 tcggcgtcac catttctgcc gaacagcaaa aaatggctca ggaacgctgt gaaggcctgg
      841 atgtcaccat tttgctgcaa gattatcgtg acctgaacga ccagtttgac cgtattgttt
      901 ctgtggggat gttcgaacac gtcggaccga aaaattacga tacctatttt gcggtggtgg
      961 atcgtaattt gaaaccggaa ggtatattcc tgctccatac catcggttcg aaaaaaaccg
     1021 atctgaatgt tgatccctgg attaataaat atatttttcc gaacggttgc ctgccctctg
     1081 tacgccagat tgctcagtcc agcgaacccc actttgtgat ggaagactgg cataacttcg
     1141 gtgctgatta cgatactacg ttgatggcgt ggtatgaacg attcctcgcc gcatggccag
     1201 aaattgcgga taactacagt gaacgcttta aacggatgtt tacctattat ctgaatgcct
     1261 gtgcaggtgc tttccgcgcc cgtgatattc agctctggca ggtcgtgttc tcacgcggtg
     1321 ttgaaaacgg ccttcgagtc gctcgctaaa ggctattcta tcgccccctc tccgggggcg
     1381 atttcagatc aggcttgtgt acctggttga ttcatggcat tttctcgtgc cgccagtaca
     1441 cgttctaccg tatctaccac tgcctgagtt tgtggatcga tttcaatgtt gacgcgtgcg
     1501 ccgagttttt tcttcccaag agtcgtgcgt tcgagtgttt ccggaattaa atggacgcaa
     1561 aaacgcgttg gcgtgacttc gccgacggtc aggctaatac cgtcgatgcc aataaatcct
     1621 ttgtacagga tatatttcat caactgacta tcctggactt taaaccagat ctgacgatta
     1681 ttttccgagg ttaatatttt cgccacttca gcggtggtca taatatgacc tgacattaag
     1741 tgtccgccaa tttcatcgct gaatttcgcc gcacgctcaa cgtttaccca atcccccact
     1801 tttaaatcgc caagattagt aatgcgtaac gtttctttca tcaggtcaaa actgacatgg
     1861 ttgccgttaa tttccgtcac ggtcaggcaa caaccgttat gcgccacgga agcaccggtt
     1921 tccagaccgt ccagcatgtg gtcgggtaac tccaccacat gcgtgcgaaa atttggtttt
     1981 tcgtcaatcg acaccagttt tgcggtgccc tgtacaatcc ccgtaaacat acttacaact
     2041 cctgaaatca gttaagacat tctgttcagc acaatagcag gtggcaaacg cccttaccag
     2101 tgaaggggta agaatgacta ttttttcact ggagaattaa taaatcctcg ctacaataga
     2161 ctgaatttcc cctgcttctt ctttttgctg cccattcagg cggcttttta gtctctcata
     2221 taactacaaa taaaaggtgt tcacgtgcag aagtatatca gtgaagcgcg tctgttatta
     2281 gcattagcaa tcccggtgat tctcgcgcaa atcgcccaaa ctgcgatggg ttttgtcgat
     2341 accgtgatgg cgggcggcta tagtgccacc gacatggcgg cggtcgctat cggtacttct
     2401 atctggcttc cggcgatcct ctttggtcac ggactgctgc tggcattaac gccggttatc
     2461 gcgcaattaa atggttccgg tcgacgtgag cgcattgcgc atcaggtgcg acaaggtttc
     2521 tggctggcgg gttttgtttc cgttctcatt atgctggtgc tgtggaatgc gggttacatt
     2581 atccgttcca tgcaaaacat cgatccggct ctggcggaca aagccgtggg ttatctgcgt
     2641 gcgttgttgt ggggcgcgcc gggctatctg ttcttccagg ttgcccgtaa ccaatgtgaa
     2701 ggtctggcaa aaaccaagcc gggtatggta atgggcttta tcggcctgct ggtgaacatt
     2761 ccggtgaact atatctttat ttatggtcat ttcggtatgc ctgagctcgg tggcgttggt
     2821 tgtggcgtgg ctactgcggc ggtgtattgg gtcatgttcc ttgccatggt ttcttacatt
     2881 aaacgcgccc gctccatgcg cgatattcgt aacgaaaaag gcaccgctaa gcctgatcct
     2941 gtggttatga aacgactgat tcaactcggt ttgccgattg cgctggcact gttctttgaa
     3001 gtaacgctgt ttgccgtcgt ggctctgtta gtgtctccgc tcggtattgt tgatgtcgca
     3061 ggacaccaga ttgccctgaa ctttagttca ctgatgtttg tgcttcccat gtcgctggcg
     3121 gctgcggtaa ctatccgcgt aggttatcgt ctgggtcagg gctcaacgct ggatgcgcaa
     3181 accgctgcgc ggaccgggct tatggtgggt gtctgtatgg caaccctgac ggccattttc
     3241 acggtttcac tgcgggagca aatcgccctg ttgtacaacg acaatcccga ggttgtaacg
     3301 ctggctgcgc atttgatgtt gctggcggcg gtatatcaga tttctgactc tatccaggtg
     3361 attggcagtg ggattttgcg tggttataaa gatacacgtt ccattttcta tatcaccttt
     3421 acggcttact gggtgctggg cttgccaagc ggctatattc tggcactgac cgatctggtc
     3481 gttgaaccta tggggccagc aggcttctgg ataggcttta ttattggcct gacgtcggca
     3541 gccattatga tgatgctgcg tatgcggttc ctgcaacgtc tgccgtcagc catcattctg
     3601 caacgagcag ctcgctagta aagacaaggc gcaaccttca cgggttgcgc ctgtgttttt
     3661 acgcaggctg gagcgttgcg ccaatcccgt cttcgtctgg ctgtagtttc agtgcgttac
     3721 acagagtttg ccgactggca tctgttatct caacgtgtat atcaccagta acttcccctt
     3781 tcacattgat gaagtcaggt gaaatttctt tcgcactggt ttgcaggcaa atcgctccct
     3841 ctccgcgcag gcgtttcact ttcagttgca catgcgttgc cgcaccagtg attagccaca
     3901 gttttccgcc attgagattg atgttaccaa cactgaaagt gacactgtca ccttttaccg
     3961 tactgccgtc gagaatcaat acaccgccct gctgtacagt aatcgttgat tgttgcagat
     4021 ctgcgccata gccaataatc attgaagcgc cgtccagtaa tgcaacactc ccggtcagtt
     4081 caaccggtgc gcgatcttcc tctacgacaa aattttcgtc cgcgctggtg ggtgacaaca
     4141 ggatcactgt cccaccttgt attaccgcat tttccagcgt accggcatgc acttcagccc
     4201 gaccacccac attaacggac aggttttgcg tcttaccgga actgtagagc tgaagaccat
     4261 ccgtacccag acgatagatg gctccattct caacaatagt gtcagagtct tcaccaccga
     4321 gattattcat ggtgccacca gcaagcaacg tatcattggc tttccccgcc agcataaaaa
     4381 cgccgccctg attaatctca caattgctgg cttccccacc tggatacacc aacaattcac
     4441 caccttcatc taccacgacc gctgaggttg tcccattgac caacaggcca ccctcttgat
     4501 cgagaataat tttttccgcg cgatgtcctt ccagtacacg caggttaccg ccattttcca
     4561 gcaacaaacc gcaggcataa ccctgatcaa cgctgaattc accttcggga tggcggccat
     4621 taaccgtagc gagcgtagag agactaattg cgcctccctc ggactgaaca atatctgtgg
     4681 caatggcacc gtcgctgacc gatagcgtgc cattgttacc gtactgtgtg catttggcga
     4741 tcccaccggt tgccactttc aaccagccag cccccgtgac cacggtattt tcggctcttc
     4801 cgccagcttc aactattaac tcaccttcag taagttgcgt cgcgccaatg acctcgccgg
     4861 tcttaacaat ttgcacgttc ccgtcgctca tcaagttttt cgcatcagat cccatgattt
     4921 attcctttgc tgcatctgtg tgcctttatt gctacctaag tgtaaaggct acggaggatt
     4981 tatccacgac agattttgag atggtggcaa acaactctgt ttaaactctg atacacagat
     5041 tattgggttg tgtcagatgt aaatgcgatc ctgaataaaa atcgcccttg caaatcaaca
     5101 aaatatagcc aatttgctta aaagctctgc aaacggtcga aatagcgcag aaaaatacgt
     5161 tttgcctctt gccaccttcc cactctgccg ctaatattcg tccccgttgt cacctacaac
     5221 gttgcgttca tagctcagtt ggttagagca ccaccttgac atggtggggg tcgttggttc
     5281 gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt tggttagagc accaccttga
     5341 catggtgggg gtcggtggtt cgagtccact cggacgcacc agattttctt aatctggtct
     5401 tctccttttt tccctctatt tcttctatgt atccaatacg ttaaaagatc tacactgtct
     5461 tcgtatgcgt tatcagaagg agaatcgcta tggcaacttt gttacaactt cattttgctt
     5521 ttaatggccc atttggcgac gcaatggctg agcagcttaa accacttgct gagtcgatta
     5581 atcaggaacc tggttttctg tggaaggttt ggacagaaag tgaaaagaac cacgaagccg
     5641 gtgggatcta ccttttctct gatgaaaaaa gcgcccttac ctatctggaa aaacatactg
     5701 cccgactgaa aaatcttggc gttgaggaag ttgtcgccaa agttttcgat gtcaatgtgc
     5761 cactgagtca aatcaatcag gcaaaacttg cctgacagat tttaatcaag ggcggttagt
     5821 gcccttttca tccctgtctg aaatttctca aattctaaaa atctcaacca aacttacctg
     5881 ataacactaa attcgaaaga atgcgtacag gtaagtaaca atgaaaacaa ttgctattgt
     5941 gggtgccggg cctacaggga tctacacctt attctcgctt ctacagcaac aaacgccact
     6001 ttctatttct atcttcgagc aggctgacga agccggtgtc gggatgccat acagtgatga
     6061 tgaaaactca aaactgatgc tggcaaacat tgccagtatt gaaataccgc cgatttattg
     6121 cacgtatctc gaatggctac aaaagcaaga agccagccat ctccagcgtt atggcgttaa
     6181 aaaagaaacc ttgcacgatc gtcagttttt accgcgaatt ctgctgggcg aatatttccg
     6241 cgatcaattt ttacgattag tagaccaggc acgacagcaa aaatttgcag tggctgttta
     6301 tgaatcatgc caggttaccg atctgcaaat tacaaatgct ggcgtcatgc tcgctacaaa
     6361 tctggattta cccagcgaga cgtttgattt agtggtgatc gcaacggggc acgtctggcc
     6421 tgatgaagaa gaagcaacca gaacgtattt tcccagcccg tggtcaggcc tgatggaagc
     6481 aaaggtcgat gcgtgtaacg tgggtattat gggaacatcc ttgagcggac tggatgcggc
     6541 aatggcagtg gctattcagc atggttcgtt cattgaagat gataaacaac acgtcatttt
     6601 tcaccgcgat aacgcaagtg aaaagctaaa tattacgtta atgtcgcgca caggtatttt
     6661 acccgaagcc gatttctatt gccctattcc ctacgagccc ttacacatcg tcactgatca
     6721 agcattaaat gctgagattc aaaaaggcga agagggcctt ttggatcggg tatttagatt
     6781 gatagtagag gaaatcaaat ttgctgatcc agactggagc caacgcatag ccttagaaag
     6841 cctgaatgtc gattcctttg ctcaagcctg gtttgccgag cgcaaacaac gcgacccatt
     6901 cgactgggca gaaaaaaatc tccaggaggt cgaacgcaat aaacgagaaa atcatactgt
     6961 tccctggcgt tatgtcattc tgcgcctgca tgaagccgta caggaaattg ttccacatct
     7021 gaatgaacac gaccataaac ggttcagtaa aggccttgcc cgggttttca tcgataatta
     7081 tgcggcaatc ccttcagagt ctattcgtcg actattggcc ttacgtgaag cgggaatcat
     7141 tcatattctc gctctcggtg aagactacga aatggaaatt aacgagtcgc gcaccgtcct
     7201 gaaaacggaa gacaacagtt actcgtttga cgtttttatt gatgcccgcg ggcagcgtcc
     7261 gcttaaagtg aaagatattc ctttccctgg actacgcgaa caattacaga aaacagggga
     7321 tgaaatccct gatgttggcg aagattatac gttacagcaa cccgaagata ttcgtgggcg
     7381 cgtagcgttc ggcgcgttgc cctggttgat gcacgaccag cctttcgttc agggacttac
     7441 ggcatgtgca gaaattggag aggcgatggc tcgggcggtc gtaaaacctg catcccgtgc
     7501 gcgtcggcgt ctttcgtttg attaaaggta aagctatcag cactcaacaa actgatgttg
     7561 ttcattaaga cgatagcgga ctccggctcg aatattgttt tcaccttcaa tggcgacggc
     7621 gaagcgaact ctttcgccgt catgatatgc gagtgcggcg catccccctg gcccgagaat
     7681 aattgagtcg accacgcccg tgctcactac tgttgaattt tcaccgctgg caatgatgtg
     7741 tacattgtcg ccgcagttgg caattctggc gtttgcgcca aagctggcga tttgcaccag
     7801 gtcaccatta ctggcgacat gacaccgttc acccaatgta caaacgcgta ctcgcattcc
     7861 ggtattcgca atccgactgc tatctccagc gctgcttatc cgcgaagaat taccggttac
     7921 agcaacccgt gcgcgtgcac cggagctgcc aatgtgactg ttaaagccga cacttccaat
     7981 ccgcacgcta taacccatac tggcaatttg cgcggcatac ccagcggttg cgattctggc
     8041 gttatcctca caacagccga tgcgagcatg atcgtcgctg cgggcaaact gactgcaaaa
     8101 tagtcgttct ccgggatctg tggccagttt ttgcatagaa gaaacttcct gatgcgcgaa
     8161 gttttcatcc gccagccatt ttttccacgc atactcaacc agactctccg cccagtccgt
     8221 atatccttcc tcaatcagtg catgatgaat atcggcataa ctaccgccac gtgggaaatg
     8281 gcgcagaaac cagcgataca tcaccgctcc gatacgccat tctcgcaggt ctgctcgtgt
     8341 aatgatcatt cgttagtgcc ggtgatagcc atcgaccata cttttaaagg tttcgtgtgg
     8401 cgtacgcccc gtggtgcaaa gataaagatg accgaagata aaaaagaggc ttataaatgc
     8461 cagagcaaaa tgcgcctgca ataaccagta tcttacgcca ggaaacacat ctcccacggc
     8521 ttgcggatag agacacagca gccccgtcaa tagtaacaac ggcagcaatc catacatgac
     8581 accaacatag gcaacctgct gtaagggatt aaatttagac tgggttgttg ccgggaaagg
     8641 atgttcttcc ccctgcataa tgccaaacaa atagaatcgc gtttgttttg ccgctcgctc
     8701 cagccacccc tgacgacgaa tgcgatagtg gtgaccatta cccccaacgg cattgatcag
     8761 aacaaagccg agccagcatg ccagtaacaa aaatccgcaa acttcatgca ccgcaaccag
     8821 acttttaacc gcagttgcgc ccaccagcgc aaagtgattt atcagcccac ttgccagcaa
     8881 caatacaaag agtaacgcat tcgaccagtg ccataatctg accgctttgc tgtaaagata
     8941 aaccttctca ccatggtcgg tggctgattt cttcacgcca cgagcacgaa gcaaggcatg
     9001 cagccccagc accaaccaca tccctaccag caatagtccg gcaatgatca accacaccgg
     9061 ccaaaattgg ggagtgaaca gtggcacata gttcgccaac tggctctgaa actgttcagc
     9121 atgttgcgac aggttcatac attttccttt ttaatcaaat gttgaccgaa ccggcgatac
     9181 agatgcggtt tcccggcacc aggtagctga tattgatagt atttattttc ctgcaaccac
     9241 gcctgaattt ctgggctatc ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg
     9301 acgcaaatgg gcggaaagcc ttttgccagt cgggactcag cgcagaaatc acatttatct
     9361 gccactttag tgacagggtt aaggtagcga acctggtaag gacaagcgcc aatgcaataa
     9421 ctgcagccga tgcactgcga tttttctacc cgtacgatcc cctgctcatc acgccacgat
     9481 gccgctgtcg gacaaacgtc gatgcacgga gcatcttcgc agtgctgaca tgactggcga
     9541 aaaaagtgat actgcgtttc gttgtcgtta tcggtgactg gaatgtgtgc tatcgataaa
     9601 cggcttccct gagcggggac gtggttagtt ttacggcatg cgcgggcgca aatattacag
     9661 ccattgcacc gtgactcgtc atgaatcatg gcataccgaa cttccttttc ttgcctcgtg
     9721 ttcgctaaca gagaagacgc agaaccagta aaaaagatta ctgttcccat ccccagaaca
     9781 aatttgcgtc gagtgaagga catctgatta tcccctaatg cagtggctgc tactgtccac
     9841 cctatccatg acatctccct gaaatttcag gggttctgaa actgctctac ctcgaaccat
     9901 tcgcataagc catacgcctt gccataggtc ataaatcgct cgctaaacaa gtacaacacc
     9961 gccgtcgatg actccacctg ccgtatatcg gcatattcag gtgcaacatc catcgcggcg
    10021 atcgcggctt caatctgcgc ttcctggaag taatatggcg cttgcatcag catggcgact
    10081 ttgtaaggac gaggatacgt ctggcactca aaacgaacgg cgtgtgcaat agcgcgacaa
    10141 atgtcctgct ccacaacttg caaagacatc gccgcgtagt tttcactcat ggcctgcgtt
    10201 gagtaatagt aatcatcctg gcttcccgtc aaacaagcga tatccgcgta gtctccgttc
    10261 tgcttaatgt tttccagcaa aactgacagt tcctcttctg caaccgaata gggcggcgag
    10321 agaaacacgg cgcgcgccac cagctgccca gccgcagaat gctgacgaat gaactctgcc
    10381 aggatttcag caggttctgg ttcggttacc gtatcaacgg ggtgacgcat cccctgcaac
    10441 cattgccgct tcgcctcatc aacttcgctg actttcgcta acggcaattc atcctgatgg
    10501 ttcatctcac ccattcctta cgcaggcagt agattgtgtg ctgccagatc ggcggcaata
    10561 tcttccagcc ccagacgctg caatgtttca cgggtcggac aaccaagctg tgggtcccag
    10621 cccatttctt tgtagaacat cgtgagcgaa gcatgcatat cgtcacgatc cattttgtca
    10681 gtaccttcag taaagaccgg gttctgcgga tccttgtcga atacccagga acagataaga
    10741 tcgtgttcat tacgcatatc tttagtttgc atcagtttta ccgtgtaggc gcgatgcaac
    10801 gtaaagatac gctctgcaac taaatctaat ttttcctgag tcatatcttc gccagtgatc
    10861 gctttgaaga atttggcttc aagcgccaga tcgccccggt aattacggct tttcagcggc
    10921 gaaacggtca ttggccagac ccagttgcac agggtgacgg cgttatgcaa acagacccgc
    10981 aacagcgacc acttagcata tttaattttt gcgtcattga ttggcgtgta gtttttggtt
    11041 tcatcgtaag catcttcaga accaaaaagt tctttcgcca cttcacgttg cagtttcaat
    11101 ggcaagccgg aaccaataaa gttgatatgg gtatgcgtca tgcagtcacg gttgaacata
    11161 cagttaacaa tggaaccgac ctgcgctgac gcttcattgg catggtgaac cggatagcca
    11221 aacggcgacc agagtttatt tttcgcgtag ccccagtact cttcacccaa attccagcgt
    11281 tctgcgatgg tatatgaacc atcagccagg tgactcagct cacccacacg atgcgccaga
    11341 cggtagtaaa aatctttaat gaagttaacg tcacccgctt ccagttgatc ccagcgaatt
    11401 tccgcatact cttcagctgg tagaacacgc ttgaacaccc ctttgctgta acaataagta
    11461 aagtcgcgat gcagctgccc atagttacac cataggccgt agtcatcgaa tagattcaga
    11521 ccaaccaggt taccaatcac acgaccatcg tctttatctt caaaatcttt cggcccgttt
    11581 gggaagatag tggtatggac aaagtttgct acacaagtgt taccaccggt gccaggcacg
    11641 ccaaactctt tcacccgagg aatattcatt tgggtcatac aacggatcgg gcaagagtgg
    11701 cagccgctca tttttactgt gtatttctct gccgccggac ctaagtcaaa aacggattta
    11761 taggtacgaa agccgaccgt attctgattg cctggcggaa tttcccccgt ttcaatcgga
    11821 ccaccttcag ccgcgcccca aaacagccct ttacgtgctg tccaacgtga cttgggatct
    11881 gaatactctg cccacgattg cggcgtactt ggcacgacat ggttgttatt cgcaccgata
    11941 agttcagtca tcatgtaatc gttgagacgc ttcatctcct gacgatcggc aatgttgacc
    12001 cctttcgtcc cttcaaccgc aatcgctttc aggtttttcg aacccattat tgcgccagtt
    12061 cccgcaccgc cgctgtggtt acggctattc aacatgccag agagaggaac aaggttttcc
    12121 ccagcctgac caatagccgc cacacaggtt tccggactgg tcaagcgaca aatttcttcc
    12181 gtcgttgcgc gcgtcccttt tccccataag aaatcggctt tttcaaggct aactttgtca
    12241 tctttaatct tcagccatac cggtgatttc gctttccctt cgataataat gacgtcgtat
    12301 ccagcgaatt tcatttgcgc tgcaaaaaag ccccccatat gggcatcgac gactaaattt
    12361 cctttggtaa aagtagaaag tgaggtgata tttacgcgag aactacaggg ggcaccagat
    12421 ccagttaatg ggccggtagc aaaaactaat ttattcgctt catcgaaagg tttcgtgcct
    12481 ggcggtactt cgtcatacat aattttgtag ccgaagccca tgccaccgac aaaactttta
    12541 aacttactgg aatcttcgag ggtaatattt cctgtcgtga gattgactct taatatatta
    12601 cctgtccaac cgttagccat gattttttcc tttgcaagat tcacacagta atatttttcc
    12661 actcgataat ttttaacgcc cctgttgggc aggcgtttgc gcattcaccg cataacacac
    12721 atttcgagga ttttttactt tcagtattta cggtggccat catccacggg catgccgtag
    12781 tacaggcgct acagccaata caacgtttat gatcgacggt aatacagcct tctttctgct
    12841 gccaggtaat cgcaccaatc gggcagacgt tcatgcattg cggctctttg cattgacggc
    12901 aggtatccgc ggtatagttg agatcgccat acaggccgcc gccagagcca accccgttgt
    12961 cgccaaagaa ataattgcga tggattttga tacgggagaa gaatgtccct actgagccat
    13021 cgttgaagtt ggtacaagag atttcacagc ggtggcaacc cgtgcatcgc gcacgttggg
    13081 tcaccagcac ccctttgggt gtatttatca accctactgt gccgctatca atatcttcct
    13141 gtttgcaacc caaaagtgac agtaaagcag gggcaattgt taaaccagca aggccttttc
    13201 ctgatatacg caagaattct aatcgcgtta agccaatatt taatagtgga cgatcaaccg
    13261 ggttcattta tttcgcatcc tcttcgcaaa cacagtgctg acaattcagc agcctgatat
    13321 taggcactca actaatacgc tgctgactct gtcctcaaca ccagatattt tccctaaccc
    13381 gatagtgtat gagaaaagat ttaacgctga taaactaatc ctttatggct aggagaaa
//
LOCUS       DAZFRG010000129        13370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFRG010000129 DAZFRG010000000
VERSION     DAZFRG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.129"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..815
                     /gene="yjiC"
                     /locus_tag="ACHKUC_003029"
     CDS             <1..815
                     /gene="yjiC"
                     /locus_tag="ACHKUC_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFO6619246.1"
                     /translation="NALQSQIITDAHFLHHPIVDSEFTRKLKYARMDSENIYLPPLTR
                     GNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHKUC_003030"
     CDS             complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHKUC_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO6619247.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1944..3404)
                     /gene="uxuB"
                     /locus_tag="ACHKUC_003031"
     CDS             complement(1944..3404)
                     /gene="uxuB"
                     /locus_tag="ACHKUC_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO6619248.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETIQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTVTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHKUC_003032"
     CDS             complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHKUC_003032"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO6619249.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIIEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5009..6352
                     /gene="gntP"
                     /locus_tag="ACHKUC_003033"
     CDS             5009..6352
                     /gene="gntP"
                     /locus_tag="ACHKUC_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO6619250.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6595..7497)
                     /gene="fimH"
                     /locus_tag="ACHKUC_003034"
     CDS             complement(6595..7497)
                     /gene="fimH"
                     /locus_tag="ACHKUC_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6619251.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7517..7960)
                     /locus_tag="ACHKUC_003035"
     CDS             complement(7517..7960)
                     /locus_tag="ACHKUC_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6619252.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8033..8563)
                     /gene="fimF"
                     /locus_tag="ACHKUC_003036"
     CDS             complement(8033..8563)
                     /gene="fimF"
                     /locus_tag="ACHKUC_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO6619253.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8573..11209)
                     /gene="fimD"
                     /locus_tag="ACHKUC_003037"
     CDS             complement(8573..11209)
                     /gene="fimD"
                     /locus_tag="ACHKUC_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO6619254.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11276..12001)
                     /gene="fimC"
                     /locus_tag="ACHKUC_003038"
     CDS             complement(11276..12001)
                     /gene="fimC"
                     /locus_tag="ACHKUC_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6619255.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12038..12577)
                     /gene="fimI"
                     /locus_tag="ACHKUC_003039"
     CDS             complement(12038..12577)
                     /gene="fimI"
                     /locus_tag="ACHKUC_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6619256.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12642..13190)
                     /gene="fimA"
                     /locus_tag="ACHKUC_003040"
     CDS             complement(12642..13190)
                     /gene="fimA"
                     /locus_tag="ACHKUC_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6619257.1"
                     /translation="MKIKTLAIVVLSALSLSSTVALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3243 a         3478 c         3241 g         3408 t
ORIGIN      
        1 gcaatgccct tcagagtcaa attattacag atgctcactt ccttcatcac ccaatagtcg
       61 acagtgagtt tacacgtaaa ttaaaatatg cacgcatgga ttcggaaaac atctatcttc
      121 cgcctcttac tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
      181 aacttgatat tagtaaagag ccttggccat ttaattatgt gacaggagca tgcagagaat
      241 ccgatggtat tacaacaaca ggacgtatgc tctatcggaa cttgaaaatc acctcagctt
      301 tagacgaaat atatggtggc atatgtaaaa aggcgcatgc taccacagag ttagcagaag
      361 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttcctggtta tgccggaacc acgataggat
      481 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaaggattg
      541 atatctatat tgatcagata atgacggtaa caggggtagc aaattcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      661 cctatcccgg taccgcggag atccatgcaa aaatgggata taaagttatc ccgggtgatg
      721 aaaatgcacc actcaaacgt atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgaatggaat tatattggta aataactacc aggaagaatg agtactaaca
      841 ttcaggttat acagatagct aaacaatacc ctgcaattta caaagtaata ctcattgcga
      901 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
      961 cgtcgacttt atccagcggc catgaatcaa acagatagcc atcaaaataa atatcgtcaa
     1021 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     1081 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1141 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attggattgt
     1201 tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta tgcgttgctt
     1261 cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc gcactggaag
     1321 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1381 cctgcaaagc ggcaaactcg gcgatgttgc tctccaataa ctgccgcgcc tgtaacagct
     1441 caaaaggacc ggcatcgttg cagacgttgg catccggact gtctgtgttc tggctgcctg
     1501 agttatcaag aacatagata ccggcacccc ggcgtacttc caccagccct ttgatctcca
     1561 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     1621 gctccggcgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga tcgcggatca
     1681 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
     1741 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1801 aacgggcaat tttacggccc ccgttaatat caaactatac cattagcact ggaagttggc
     1861 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1921 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1981 cacagctgct gatatgccgc cgtcactgcg ccaacaaagt cggcattctg cggcagatca
     2041 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2101 gcgttgatct tctggaactc cgccagcatc gggtcgacca cgtcaatggc attaccctgc
     2161 tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgccag gtgacgccag
     2221 ctgccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg taacttctgg
     2281 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2341 atcagcagcg tcgcataggc gttcaggtct gtaccttccg gcatcgacag cgttggcgct
     2401 tgttcctgca tcatcagggc aaaggccgct ttgcgataag ccgggttagt cacggtgtcg
     2461 gcaatggttt catagccgcc gaggtaaccg aggtacgcca gaaaagagtg gctgccgttc
     2521 agcatacgca gcttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     2581 ttatcccaat ccgggcgacc attaacgaag ttatcttcaa tcacccactg acggaacggt
     2641 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtatggtt
     2701 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacattttct
     2761 tcaatccatg ccgccagctg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     2821 gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaacgc tttcaaccct
     2881 ttttcacgac gcagacgcaa cgcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2941 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     3001 tcagcgcagt agcctttttc cgtgaccgtt agagagacaa tcgccgtttg cggacgcgcc
     3061 atcgcgttga gaataccttc gcagccatcg atttccggat gcagcgcttc tttcatcgaa
     3121 ccgataattt tcagctcggt gctctctgcg cctttttccg ctacggtgta cagcagttgc
     3181 tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag
     3241 atgccccagt cgctgtcggt gctttccagc agatgatggg tatacagcgc ctggtgcgcg
     3301 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3361 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3421 cgtcttcaaa gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     3481 gatattaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     3541 ggcctttcag acgaccaatt gcggagtaac ctgggttggt tttcttcttc aggtcgtcca
     3601 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3661 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     3721 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3781 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     3841 cgccgtagga accggtgcac atggtaaaac cgtttgccat gctgtttacg gtatcaacca
     3901 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     3961 gatcgtccgg gtgaacagcc atacgcacgc cgacttcttc agcaactgga ataatcgctt
     4021 tcaggaagac ggcaaagttt tcgcgcagct tcgctttgtc gatatctttg tacagctcca
     4081 ggtgtttacg gaactggtcg agggtgtagc cttcttccgc gcccggaaga ccagcaatga
     4141 tgttacgggt cagacgcgct ttgtcttcat cgctcatagt ggcgaagcgt tcagctgcct
     4201 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg gcgtttcagg atatgcattt
     4261 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4321 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4381 tgccacactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     4441 tgccagtgtg ggttttgata tcttcgtgaa ttggcacgct ttctacgaca gaccacacca
     4501 ggcctgcgtc ttcaatgatc gccttgcgtt tgaggatctc ttcaacggac catacttcgc
     4561 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4621 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4681 tttcacatag tgggttccac agcaaagaga cgcggaaccc tggagttgtt taggtatccg
     4741 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4801 cttttggtaa gacatgtcaa cccgaattct gaaattggtt aaccacatca caagaatttc
     4861 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4921 atgtgacatt catcgcaaca atggttgacc aatttacata acatatcgcc caaaataaca
     4981 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     5041 cggcattggt ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5101 gttggtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     5161 cactatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     5221 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cgcatacgct
     5281 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gcctgatttt
     5341 tggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgttat
     5401 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     5461 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     5521 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     5581 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5641 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
     5701 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     5761 agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg caatgtttat
     5821 tgcgatggtg gttgcattcg tactctttgg caccgcacgt ggtcatgaca tgcagtgggt
     5881 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     5941 gggtggcgtg ctgaagcaga ccatcatcga caccggcatt ggcgacacca tcggcatgtt
     6001 gatgtcccac ggcaatatct cgccctacat catggcatgg ctgatcactg tgctaattcg
     6061 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
     6121 aatcctagat ccagcaactg gtcagctggt tggcgtgaat ccggcgctgc tggtactggc
     6181 gacggctgcg ggttccaaca ccctcaccca cattaacgat gcatctttct ggttgttcaa
     6241 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt
     6301 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaaggacac
     6361 cagagcctgc caatggcagg ctcagactga tgacaaagtc taaaaatgtg cccggacagt
     6421 cccctcgccc ctccggggag agggttaggg tgaggggaac agaccagcaa agtgcgcttt
     6481 gtttatgcca gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg
     6541 tttttagctt caggtaatat tgcgtaccag cattagcaat gtcctgtgat ttctttattg
     6601 ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct gccctccggt
     6661 acgtgcataa tttgccgtta atcccagact caccgccgaa gtccctactg ctcctaacga
     6721 taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg
     6781 tgcaggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg cggttgtgcc
     6841 ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag gaattggcac
     6901 tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat cgcagccgcc
     6961 agtaggcacc accacatcat tattggcgta aatattccac acaaactgga aatcatcgct
     7021 gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag ctttaatcgc
     7081 caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt
     7141 tctcgaatta taaacaacgc gcggcgtttc gctggtggta ggaaatggat agctactgcc
     7201 actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg agcctcgttg
     7261 cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga
     7321 aagatccacg accaggtttt gccccacatt cacgacgggc gcaaggttta cataaacatt
     7381 ggcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg cgaatgacca
     7441 ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc gtttcattac
     7501 aatcatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc tgaatggttc
     7561 cctgagtggc tccgccattt actgtcaatg ctctgacctg taacgggaag tgcgctgatt
     7621 gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg ttgccactgt
     7681 catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtac
     7741 tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga caattagtca
     7801 actcaagcgc aacatcatgc caggccgatg ccgccccggc agacataaga ctgaaagaat
     7861 aaagatcgcc gagatcaacc gtggcattgg tggtggaaac cgtacacggt ttggcgacga
     7921 ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca
     7981 atattgccgc caatacatac ccacgtttgc accatttcat gagcatctcc agttactgat
     8041 attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc acctgtgtcg
     8101 ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg gtcgtccacg
     8161 aaagcgcgga cgatggagca ttaaggggta tttgattttg ctgctcattc agaagctgta
     8221 ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg gcattgtggc
     8281 tatctgcaac gccagtaaac ccaaccttta cggcagaaac ggcattacca cagggtgaca
     8341 gcaaaatacg aaatggaaca acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg
     8401 cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta cagccgttat
     8461 ccctgacata gccgcggata gtaatcgtgc tatccgcagc caaagcgtga ctcaccgcca
     8521 gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc ccttaacgac
     8581 attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt tgataattgg
     8641 cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca
     8701 ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg
     8761 atgtcaccat cgccccaaac ggcagcggct tattattgtg ggtcagcgtc atgagcagtt
     8821 ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg ggaacaacgt
     8881 tagcaaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc agcgccactc
     8941 tattttcccg atattcagtg gcataaggca gcacggcata accacgccag tcggtacgca
     9001 cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca agcaccaccg
     9061 tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc ccaccgctga
     9121 ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg gcattgccgt
     9181 aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctatttcca tcgcctcccc
     9241 cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac gtaccgtata
     9301 caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag ctggcactgg
     9361 catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga atattgacgt
     9421 taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg ctatagctga
     9481 gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc tcatcgacat
     9541 tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat gtgcgcccga
     9601 gttgctgagt aacggtgagt tgtaatttcc cgcgtttgtt ataagcgagg ttgtaatagt
     9661 cggtgaattt cggcttaacc tgaataactc cgtcctgtgt ttcgatgttg tagccattca
     9721 ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa cggtaaccca
     9781 ctaactgaat attcgtgcct gattcattga gcgatttgtt atagagaaaa cgcaccgatt
     9841 gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata tccacagaca
     9901 gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga taacgatccg
     9961 ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt aatgtactct
    10021 ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct gccgtaatgg
    10081 aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt acggtaaaaa
    10141 tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta ttacctgcgg
    10201 catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa atgtcatacc
    10261 cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc accggggcaa
    10321 atcctctttg actatcgggt aacatattgt catctgaggc caattgtgcg ccgcgaaagt
    10381 taataccatc gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc cgggaacgta
    10441 acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt ttgctacctg
    10501 atgatctgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc cacgcaccaa
    10561 tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca atccgattct
    10621 gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg ggatcccata
    10681 actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt
    10741 tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc ctggaccatt gtggttaatg
    10801 gcacacaggc atcatccgcc agcagattca taccggcgac agaagccgta ttcagcccca
    10861 tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg tcgcccgtat
    10921 taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg acgcgatacg
    10981 tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca gcctggggat
    11041 catccgctaa aaagcgcgga ttaaaataga ggtcggcaga tgacaaaggt gcctgtgcgg
    11101 caaaagcaca ggcgacaacg agtcggacaa aaaaaccagc caaacgatgc ttacgaatat
    11161 gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt attcctccgg
    11221 cttaaaatca cccggcagtc aattcttttt attcaggcga aaaattcccc ctgcgttatt
    11281 ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt cggtaagtaa
    11341 tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga ggcaccaatg
    11401 cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa tagggtgtcg
    11461 ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct gcggcctgat
    11521 cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg ataattgcga
    11581 gctgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct ttaacgttca
    11641 tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca agaatacgta
    11701 aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata aaacgaccat
    11761 cctttacacc atcggcattt tccacccatg attgaattaa ataggtacta ttttcatcat
    11821 tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg cgagtcgcac
    11881 ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac atcaggatac
    11941 ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt ttattactca
    12001 ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt aaagagaacc
    12061 aggcctgggc attggcgatg ccgccagtaa cccgacgccc ggtagcacga tatttggcga
    12121 tgaaatgtag cgaagtagag cctgaataaa gccgtttcca gtttgctgga ggacgattaa
    12181 tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg gctatccctg
    12241 gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg tgaaacgcca
    12301 cacctacacg ttcactcacc accgtgctac attcccgtaa atgaataaca aaaggcaccg
    12361 gtgcgctatc ttccccaacc gcatgaaacc ggttactgct gatttgcccc atattgaccg
    12421 tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg ccctgaaatt
    12481 gcatatttcc gccgggcaac gtggtattcc atttatttcc ggccagagca aacattggca
    12541 gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc gcacaagggt
    12601 gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata ctgaaccttg
    12661 aaggtcgcat ccgcattagc agcacccggg gttgcggccc cggttgcaaa ataacgcgcc
    12721 tggaacggaa tggtattggt tccgttattc agggttgttt ctgaactaaa tgtcgcacca
    12781 tccagcgtca gcgcagcacc cgttctgtcc aggatctgca caccaacgtt tgttgcgcta
    12841 cccgcagctg aactctgcag agccagaacg ttggtatgac ccgcatcaat cgccgtacct
    12901 aaaaaggcaa cagcggcttt agatgcaaca ttggtatcgc aatcattcag ctgaatgtta
    12961 aaaccgacag cagaactggt tgctccttcc tgtgccagcg atgcggtacg aacctgtcct
    13021 aactgaacgg tttgatcaac agagcctgca tcaactgcgc aagcggcgtt aacaacttcc
    13081 cctttaaagt gaacggtccc accattaacc gtcgtggcag cggccagagc cactgtagaa
    13141 ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat gctgctttcc
    13201 tttcaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt gttttgtcaa
    13261 cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt tggggccatt
    13321 ttgactcata gaggaaagca tcgcggacaa actttttcag tttatttgtt
//
LOCUS       DAZFRG010000130        13281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFRG010000130 DAZFRG010000000
VERSION     DAZFRG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.130"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..322
                     /locus_tag="ACHKUC_003041"
                     /pseudo
     CDS             149..322
                     /locus_tag="ACHKUC_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(555..1418)
                     /gene="potB"
                     /locus_tag="ACHKUC_003042"
     CDS             complement(555..1418)
                     /gene="potB"
                     /locus_tag="ACHKUC_003042"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO6619258.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPGAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(1402..2538)
                     /gene="potA"
                     /locus_tag="ACHKUC_003043"
     CDS             complement(1402..2538)
                     /gene="potA"
                     /locus_tag="ACHKUC_003043"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO6619259.1"
                     /translation="MGQSKKLNKQPNSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIECLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2788..4014
                     /gene="pepT"
                     /locus_tag="ACHKUC_003044"
     CDS             2788..4014
                     /gene="pepT"
                     /locus_tag="ACHKUC_003044"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO6619260.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(4063..5184)
                     /gene="roxA"
                     /locus_tag="ACHKUC_003045"
                     /gene_synonym="ycfD"
     CDS             complement(4063..5184)
                     /gene="roxA"
                     /locus_tag="ACHKUC_003045"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO6619261.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(5260..6720)
                     /gene="phoQ"
                     /locus_tag="ACHKUC_003046"
     CDS             complement(5260..6720)
                     /gene="phoQ"
                     /locus_tag="ACHKUC_003046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO6619262.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(6720..7391)
                     /gene="phoP"
                     /locus_tag="ACHKUC_003047"
     CDS             complement(6720..7391)
                     /gene="phoP"
                     /locus_tag="ACHKUC_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO6619263.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(7561..8931)
                     /gene="purB"
                     /locus_tag="ACHKUC_003048"
     CDS             complement(7561..8931)
                     /gene="purB"
                     /locus_tag="ACHKUC_003048"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO6619264.1"
                     /translation="MELSSLTAVSPVDGRYCDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(8935..9576)
                     /gene="hflD"
                     /locus_tag="ACHKUC_003049"
     CDS             complement(8935..9576)
                     /gene="hflD"
                     /locus_tag="ACHKUC_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO6619265.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(9612..10718)
                     /gene="mnmA"
                     /locus_tag="ACHKUC_003050"
                     /gene_synonym="trmU"
     CDS             complement(9612..10718)
                     /gene="mnmA"
                     /locus_tag="ACHKUC_003050"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO6619266.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(10772..11233)
                     /gene="nudJ"
                     /locus_tag="ACHKUC_003051"
     CDS             complement(10772..11233)
                     /gene="nudJ"
                     /locus_tag="ACHKUC_003051"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO6619267.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(11243..11896)
                     /gene="rluE"
                     /locus_tag="ACHKUC_003052"
     CDS             complement(11243..11896)
                     /gene="rluE"
                     /locus_tag="ACHKUC_003052"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO6619268.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWREATD"
     gene            12068..>13281
                     /gene="icd"
                     /locus_tag="ACHKUC_003053"
     CDS             12068..>13281
                     /gene="icd"
                     /locus_tag="ACHKUC_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO6619269.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLK"
BASE COUNT         3307 a         3339 c         3408 g         3227 t
ORIGIN      
        1 ttatagtttt atttgtgaat taaatcaatt atggcgatga attacaaggg gttaaatgct
       61 gccgcagcat agcgatattg aaatagcctg gtatgcttcg atacagcagg agccgaatgg
      121 ctggaagacc gtcaccacac agttctacat ccaggaattc agtgagtata ttgcgccact
      181 gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa agatcgttgc tggaggcatg
      241 gaataaatat cgggtattgt tgaatcgtgt tgatacatca actgcacctg atattgagtg
      301 gccgacttca cctgcagagt aatcaacggc gcagggatga tattttgtgc ttgaagtgcc
      361 tccccgacca ttcagatagt cggggagggg tattgggatt atacgcaatc agttttagtt
      421 aaagaccgaa gctctcgaaa aatctttctc caaaaccaga atggaattga ttgattttga
      481 taggatctat aacgtcgtta ccttaaacgg caaaaccact caaaaaacaa acttaatgca
      541 ttgaatataa aggattaatc atctaattcg ctcaccttct tattcagcag acgagaagcg
      601 cgccagtaaa ccagcaacat caggcccatt acgatagtca gcgtaatgct ggtggctgca
      661 ccaaacggcc agtcacgaat attgaggaac tgtaccttga tgacgttacc gatcagcagg
      721 tttttcgcac cgcccatcag gtcggacaca tagaacaggc ccatcgctgg cagcatcacc
      781 agcagacatc cggcaataat tcccggcatc gttagtggga taatgatgcg gataaaggtc
      841 tgcaacttgc tggcaccgag atcgcgcgcc gcctcaagca acggcttatc cagtttttcg
      901 atactggagt acagcggcat caccataaac ggcagcagaa tgtaaaccag accgataatc
      961 accgcaccag gcgtgaacat gatgcgaatt ggtgtatcga taacgcccag ccagagcaaa
     1021 aactcgttga gatagccttt ggtgctgagg aaaattttca gcccgtaaat acgaatcaat
     1081 gagttggtcc agaacggaac aatcagcaga aacagcagca gcggacgcac cttgtgtggc
     1141 agcttcgcca gaaaccaggc aaaaggatag cccagcacca ggcaggcgag ggtggcgatc
     1201 agcgccatat tcagcgagtg caatagcact tcaaaataga gcggatcgag cagacgcgtg
     1261 tagttatcca gcgtaaagac cattttgacg aaactggcgt cgtcgcgggt caaaaagctg
     1321 gtgccaatga tcatcaggtt gggcagaaag acaaacaaca caagccaacc gacaatagtg
     1381 acaatcacta cattctggaa cttacttgtg ttcttcatca gccagtacga cctcccagct
     1441 ttctacccaa ttaatggcca ttttttggtc gagagagtgg tcaaagtcag gatcgtcttc
     1501 attgaagaat tcgctgacca tcaccatctt gccattttcc agctcaacaa ccgactccag
     1561 cgtcatgcct ttgtagttac gctcgcgaac gtaaccaatc agcccttcgg cgtggttgtc
     1621 gtcgttaatc tcttcaacac gtaagtcttc cgggcgcagc agaacatgca gtttttgccc
     1681 cggttcaacg gcgaagttaa cgtagatatt acattcgcgg ccttcaacgt tggcgcgtac
     1741 gcgctgctcg tcgagacatt cgatgacagt ggcgttaaac atattgattt cgccaataaa
     1801 cccggcaaca aacaggtttt tcggctcttc gtagatttca cgcggcgtgc cgtcttgctc
     1861 aatgcgacca tcgcgcatca ccacgatcct gtccgacatg gtaagtgctt cttcctggtc
     1921 gtgagttacg aacacgaatg taatgccaag cttacgctgt aacgctttca gctcgttctg
     1981 catttgcttg cgcagtttgt aatccagcgc tgagagcgac tcatccagca acaacagacg
     2041 aggcttgtta accaccgcgc gagcaatggc gacgcgttgc tgttgaccac cagagagctg
     2101 atgcggtttg cgttgagcga aggtttccag ctgcaccatc cgcagggctt ccatcacgcg
     2161 gggcgtaatt tcagcagcgg gggttttttg catgcgcaac ccaaaggcca cattttcgaa
     2221 cacggtcatg tgggggaaaa gtgcgtagct ttggaaaaca gtgttcacat agcggttttc
     2281 cgccggaacg tgggtgatgt cctcgttatc cagcatgatg cgtccggaat caacagtttc
     2341 cagacctgca atcaggcgaa gaacggttgt tttaccgcag ccagaagggc caagcagcgt
     2401 gaggaactcg ccattgttga tagtcagatc cagctgggga atgacctctt taccatcaaa
     2461 gcatttgcga attcccgcca attgcaccag cggtgaaagc gaattcggtt gtttattcaa
     2521 ttttttactc tgtcccatgt aaacgcaacg gatggcttac cgatgcgggg tttgtggtta
     2581 accaccttgg tgactcttaa tgagggcggt aattctacgg caaaccgctt gaatcgccaa
     2641 tctttgttgt gaattactgg cttagcttta tattcattaa ggtaatgctg ataaatattc
     2701 ccgcttgcag ggctaaaagt gacctgacgc aatatttgtc ttttcttgct tcttaataat
     2761 gttgtcacaa aaagtgaggg tgactacatg gataaactac ttgagcgatt tttgaactac
     2821 gtgtctctgg atacccaatc aaaggcaggg gtgagacagg ttcccagcac ggaaggccaa
     2881 tggaagttat tgcatctgct gaaagagcag ctcgaagaga tggggcttat caatgtgacc
     2941 ttaagtgaga agggcacttt gatggcgacg ttaccggcta acgtccctgg cgatatcccg
     3001 gcgattggct ttatttctca tgtggatacc tcaccggatt gcagcggcaa aaatgtgaat
     3061 ccgcaaattg ttgaaaacta tcgcggtggc gatattgcgc tgggtatcgg cgatgaagtt
     3121 ttatcaccgg ttatgttccc ggtgctgcat cagctactgg gtcagacgct gattaccacc
     3181 gatggtaaaa ccttgttagg tgccgatgac aaagcaggta ttgcagaaat catgaccgcg
     3241 ctggcggtat tgcaacaaaa aaacattccg catggtgata ttcgcgtcgc ctttaccccg
     3301 gatgaagaag tgggcaaagg ggcgaagcat tttgatgttg acgccttcga tgcccgctgg
     3361 gcatacaccg ttgatggtgg tggcgtcggc gaactggagt ttgaaaactt caacgccgcg
     3421 tcggtcaata tcaaaattgt cggtaacaat gttcacccgg gcacggcgaa aggagtgatg
     3481 gtaaatgcgc tgtcgctggc ggcacgtatt catgcggaag ttccggcgga tgaaagcccg
     3541 gaaatgacag aaggctatga aggtttctat cacctggcga gcatgaaagg caccgttgaa
     3601 cgggccgata tgcactacat catccgtgat ttcgaccgta aacagtttga agcgcgtaaa
     3661 cgtaaaatga tggagatcgc caaaaaagtg ggcaaagggt tacatcctga ttgctacatt
     3721 gaactggtga ttgaagacag ttactacaat atgcgcgaga aagtggttga gcatccgcat
     3781 attctcgata tcgcccagca ggcgatgcgt gactgcgata ttgaaccgga actgaaaccg
     3841 atccgcggcg gtaccgacgg cgcgcagttg tcgtttatgg gattaccgtg cccgaacctg
     3901 ttcactggcg gttacaacta tcatggtaag catgagtttg tgactctgga aggtatggaa
     3961 aaagcggtgc aggtgatcgt ccgtattgcc gagttgacgg cgcaacggaa gtaagcgaaa
     4021 agggatgcgg catgtgatgc cgcatccggc ttaaatccaa acttaccctt cgaagaacca
     4081 gtatccgcta ttgaccagcg ccgcgagcat cgcgaggaat gacggatctt ccagcgcatc
     4141 gccaaaattc tccgcggtca gcgcaatgtt gctggcgagc gcatccagtg ccggacggtg
     4201 cggggaatcg atcttctcac cattggcata cacgtcgtcg ccaatgcgca atacgcgcag
     4261 accccccagg cgcaccagca catcaccttg tttcagcgca tcgtagattt catccggctg
     4321 ataaggcggc tctggcggcg cgatgtccag ttcatgacgt gactgggata taaactcgcc
     4381 aaaccattgt ttaaagtgtt ccggctggtt gatcaattcg agcatcatct cacgcagttt
     4441 atccatctct tgcggcagaa cgtccgcagg atgagcgcga ggtggaacat caggatcgct
     4501 gtagtagttg ccgcccagtt cacgttgcag cacataatcg gcaaatccgc taatcagttc
     4561 ccgcgtattt ggcgcgcgaa aacccacgga atagttcatc gcattttcca gcgcgtagcc
     4621 ttcatgcggg aatcctggcg gaatataaag aatatcgcca ggctccagct cttcatcgat
     4681 gatggcttcg aacggatcga cctgtaacag gtccgggtgc gggcagtgct gtttcatttg
     4741 cagcttttcg cccactcgcc agcgacgacg accggtaccc tgaatgataa acacgtcgta
     4801 ctgatcgaga tgcgggccga cgccgccgcc cggtacagaa aaagagatca tcagatcatc
     4861 aatacgccag tccggtagtt cacggaacgg tcgcattagc gcggcggtcg gctcatgcca
     4921 gtggttcact gcttgcacta acaatgacca gttagtttca ccgagatgat cgtagctttc
     4981 gaatggaccg tggctgacct gccatttgcc atcctgatga ctgaccagtc gactgtcgac
     5041 ttcgctttcc atcgccagac ccgccaactc gtctggagag attgggtcaa taaaattatt
     5101 aaagccgcgt tttaacacca ccgggcgttt ctgccagtga cgttcaagaa aatcgggcca
     5161 gttaagagtg agttggtatt ccatgttaag catccacagg ctggtatctg caaccgatta
     5221 taacggatgc ttaacgtaat gcgtgaagta tggacatatt tattcatctt tcggcgcaga
     5281 atgctggcga ccaaaaatca cttccatccg cgcaccgccc agcatgctct ctccggcgac
     5341 gattttaccc tcatattgct cggtgatttc gcgggctacc gccagcccca caccttgccc
     5401 agggcgtaaa gtatcaaccc gttgaccacg gtcgaaaatg acctctcgct tgcttaatgg
     5461 aataccgggg ccatcatcct cgaccacaat atagagatgc tcgtcggttt gccttgcaga
     5521 aatttcgaca aactcgaggc aatatttaca ggcgttatcc agcacgttgc ccatcacttc
     5581 gacaaaatca ttctgctcac caacaaagct gatttctggc gagatatcga gagagatatt
     5641 gacccctttg cgttgataca ctttgttcag cgccgaggtg agattgtcca acagtggggc
     5701 gaccggatgc agctcgcggc taagcaatgt cccgccgcgc atactggcac gatgcaggta
     5761 gtagccaatt tgctgtgaaa tgcggctgat ttgctccagc attaccggct cagcatcact
     5821 gacgctcatc ttctcactac gcagagaacg cagcgtactt tgcagcaccg ccagtggcgt
     5881 tttcagacta tgggtcaggt cggtgagcgt cgtacgatat ttgtcgtaac gttcgcgttc
     5941 actttttaac agtcggttca ggtttcgtac cagactggtc agttctcgcg ttgtggctgg
     6001 attgagcaat tcacggttat gttcttccag ttcgcggact tcttttgcca gggcttcgat
     6061 ggggcgtaaa ctccaccagg cggcgaccca cagcagcggg atcactaaca gcagattggc
     6121 tgagagcaca tagataaacc agctccagac catataggaa ctttttagct ccaccggaat
     6181 ggtatccacc accacaatgg ttaactttgg catccgcgat gttgccgggt agacgtttac
     6241 cgccaccgag tgggtcatct ccgcgtcgtc atcatcttcc cgcacttcct gcaactgttg
     6301 ctgtatcgaa tgatctccac tcagcaagag gctggtatcg ttaacatccg cttcaatttc
     6361 atgaaaacca ttcgatttca gccagtcagg ctggatcatc ttcatcagcc agggcacgtc
     6421 acgttgcgcc cataaaagct gcccgttctc atcataaatt agtgtcatcg tggggctttg
     6481 cttgtcgata ttttcgggta actcgacatg caacttattg ttttcccact tcgcaagggt
     6541 atagaacaga ttgctctcgc cacgtaacag ccgaaacgtg gttttatcga aactgacgct
     6601 ataaccgatc agcgcaacca ttccgtaggc aagcgaaagc accaatacta ctgctgccgt
     6661 tgctaacaga aaacgtaccc gcagcgagag cgggaaaaaa agacgcagta attttttcat
     6721 cagcgcaatt cgaacagata gccctggccg cgaacggtgg taatcacttc ttgcggatat
     6781 tgtgcctgta tttttttgcg cagacgtccc atcagtacat ctatggtatg actttcccgc
     6841 agctccgcat ccggatagag ttggagcatt aacgaatctt tgctcaccac tttgccatta
     6901 ttgcgtatca acgtttccat aatggtgtat tcgaacgcgg tcagtttgat cacttcgtca
     6961 ttaatagata attcacggcg ggagagatca acctgaaacg ggggtagcga aatgacctgt
     7021 gaagccagac cgctattacg ccgcattaat gcctgcattc gcgccatcac ctcttcaata
     7081 tgaaacggtt tagtcacgta atcatcagca ccggcactta atacttcgac tttgtcctgc
     7141 cagctttcac gggcggttaa taccagaatc ggcagtgaaa catcattgct acgccagcgg
     7201 cgaatcagtg acagaccgtc ctcgtcgggc aaaccgagat cgacaatcgc aatatccggt
     7261 aaatgttcat tgagataata atcggcttct ttggcatctt ctgcgtcatc gacctgatga
     7321 ccagcatcct gaatctgaac tttaagatgg tgacgtaaca acgcattgtc ttcaacaacc
     7381 agtacgcgca ttttttattt ctccctgtct taattattaa aatagtgtaa cgcgattatg
     7441 tggttatggg ggtaaacatt aaataaacca gcggggaggg gaggtaaagt gaaaaaataa
     7501 aaagtggata atcttaataa gcaggccgga cagcatcgcc atccggcact gttttgaggc
     7561 ttatttcagc tcatcaacca tggtgatggc gcgaccaatg tagttagccg gcgtcatcgc
     7621 tttcaggcgg gctttctctt cttctggcaa cgccagacca tcgataaact gcttcatgcc
     7681 ttcggcgtca acgcgcttac cgcgagtcag ctctttcagc ttctcgtacg gtttttcgat
     7741 gccatagcga cgcataactg tctggattgg ttcagccagc acttcccagt tgtgatccag
     7801 ttcatccagt agatggtcac ggttcacttc cagtttactc acgcctttca gggtggattg
     7861 atacgcaatc agcgcataac cgatacccac gccgaggtta cgcagcacgg tggagtcggt
     7921 caggtcacgc tgccagcggg aaactggcag tttgcttgcc aggtgctgca ataccgcgtt
     7981 ggaaagcccc aggtttcctt cggagttttc gaagtcgatc gggttaactt tatgcggcat
     8041 ggtggaagaa ccaatctcac cagcaatggt tttctgtttg aagtggttaa gggcgatata
     8101 accccagacg tcacggtcaa agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa
     8161 cagttcggca atgtagtcgt gcggttcgat ctgggtggtg tacgggttcc actgaatacc
     8221 cagcgaggtg acgaactctt cgctgaactg atgccagtca acttccgggt aagcggcgat
     8281 gtgggcgttg tagttaccga ccgcaccatt gattttgccg aggatctcca cctgattaag
     8341 ctggcggtac tggcgctcca tacggtaggc gacgtttgcc atctctttac cgatggttga
     8401 cggcgtggct ggctgaccgt gggtacggga cagcagcggg atatcgcgat actgaacggc
     8461 gagatcttta atgccatcaa tcagttgacg ccagtacggc aggatcactt catcacgcgc
     8521 ggttttcagc attaatgcgt gggagaggtt attgatatct tccgaagtac aggcaaagtg
     8581 gatgaattca gaaaccgcgt gcagttccgg gatctccgcc actttttctt tcaggaaata
     8641 ctcaaccgct ttaacgtcgt ggttagtggt acgctcgatg gttttgatgc gtgctgcatc
     8701 ttcttcgctg aaactggcga caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc
     8761 aggaacttcc ttgatcgctg cgtgcgcggc cagtttttgc agccaacgta cttcaacttg
     8821 tacacggaat ttcagcaaac cgtattcgct gaaaatcccg cgcagcgcgc tgactttatc
     8881 gcagtagcgt ccatcgacag gggaaacggc ggtcagtgag gataattcca tagatcacaa
     8941 ctccggggtt aaatgagcaa gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa
     9001 catcagttgc agacgaccgc cgccgacctg gtgccagagc acggcggcgc gaatgcctgc
     9061 cagcagggtt gcgcgaactt tcgcctgcac ctgtgggctt tgcagtacag caggggaacc
     9121 agtgacctga atgcgcgggc caagcgggct gataacatca acatagatgg cagccatcgc
     9181 gctcatcagc gtttcggact gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat
     9241 ccggttgccc agagtgtcga gcgcaccttt cgctgaggag agtttgcgct caagcaccat
     9301 cagactgagt gtgtagcggg ttaattcggc gtttaagccc tggcgactgc tggcattgag
     9361 tacgccgagc aaggtttcca gcccgacgcg caggttggct tcgctaccgc caaaaaccgc
     9421 cagcgttgag ctggggttca tatcgataat actgttgagt gagacgtgta gcgcatcgcc
     9481 atcacaatgc ccctggtgag cgagttgttg caccaggcgt gccgactgac aaataccggc
     9541 aagggcgagg gtgatgtcat agtaattctt tgccacgttc actgcttcct tgttttaagt
     9601 aaagataata atcagaccgg cagcggcaga cgctgctcaa taataccgcc accgaggcac
     9661 acttcaccgt tatagaaaac ggcagactgg cccggcgtca cggcggcaac cggctcatcg
     9721 aaaatcactt caatgcgatc agcgtccagc gccttgacgg tgcacgggat gtcggtctgg
     9781 cgatagcggg tttttactgt gcaacgcata gtaccggtga acggctcgcg atcgacccag
     9841 tgcagctgct gggcaatcag accgacagac atcagacgcg ggtgttcatg gccttgcgca
     9901 acaatcagaa tgttgttttc gacgtcttta tcgaccacat accacggttc ttcggtgccg
     9961 tctttggtgc caccgatacc cagaccttta cgctgaccga gggtgtgata catcagcccc
    10021 tggtgctcac caatttcatc gccatcgacg gtaatgattt tgcccggttg cgccgggaga
    10081 taacggccca ggaactcgcg gaatttacgc tcgccgataa agcagatacc ggtagaatct
    10141 tttttcttcg cggtgaccag accgagatct tcagcaatct tacgcacctg cggtttttcc
    10201 agttcgccga ccgggaacag gctttgcgca atttgctcat ggctgagcgt ataaaggaag
    10261 tagctctggt ctttattgct gtccagacca cgcagcaggc ggctcttgcc atcgacatct
    10321 gcccgacgta cgtaatgacc ggtagcgata taatcggcac ctaaatcttc ggcggcaaat
    10381 tcgaggaaag ctttaaattt gatctctttg ttgcacagaa tatccggatt tggcgtgcgg
    10441 ccggctttat attcggcaag gaacagttcg aagacgttgt cccagtactc ggcagcaaag
    10501 ttaacggtgt gcagttcaat gccgagcttg tcgcagacag cctgggcatc agccagatcc
    10561 gctgccgctg tgcaatattc ctcaccgtcg tcttcttccc agttcttcat aaacaggcct
    10621 tcgacctgat atccctgttg ttgcaacagc caggcagaaa cggaggaatc gacaccgccg
    10681 gacatgccga cgattacttt ttttgcggtt tcagacattg gatcactcac gacattgaac
    10741 ttcaaggcgg cgtattctat cacgcagcct tttagatgac accctttgta aaaggccagt
    10801 taaaatcgcc aatcatctcc agcggatagc gttgcccgct ttgataacaa cgaatacttt
    10861 ccgccaccag cggcgaacga agatttgacg cctgtaaaat ttcttcggcg ctgacccaac
    10921 ggcagcagtc gatatcgctg tcatgaggct gcgtcgggca tatttgctca agctcaatgg
    10981 caaagaggaa acgcaaaaac ggcgttttat ctggcgcaat ccactgatgc atacgaataa
    11041 agtgttgcgg ctgcgcgctg atgccggttt cttcccacag ctcacgggcg gcggcttcca
    11101 ctaaggtttc atcggcttcc aggtgcccgg caggctggtt ccataacgct ttaccattaa
    11161 tcgtctcttc aacgactaaa aatttgcctt ctgcgtgcac cacgcaggca acggtaacgt
    11221 gcggtttaaa cattttgcat ccttaatctg tcgcttctcg ccattcgcca ttggcaagat
    11281 tatccaaaga gtaattcccc atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat
    11341 gggcggtcat gcggcgcacc tggcgattac gtccttcata taaggtgatc ttcagccagc
    11401 tggtgggaat acttttgcgc tcacgaattg gtggattccg cggccataac cacgcgggtt
    11461 cttcaaccag ctccgcgcct gcgggcaggg tagggccatc atttaaggtt acaccattgc
    11521 gcaaggcttc aagtgcgtct tgtgtgggaa taccttccac ctgcacataa tagatttttc
    11581 cggtgcgttt acccggctgg gttaaacgcg cctgcaacgc gccgttattg gtcagcacca
    11641 gtaacccttc gctatcgcgg tcaaggcgac ctgctgcata aacgccttga accgggatga
    11701 attcttttaa tgttttgcgt ccggcttcat cggtgaactg cggaagaaca tcgtagggtt
    11761 tattgaacag gatcacacgc gtgggctggt tgtcaggttt tcgtctggta gaacgttgcg
    11821 agctgaatcg cttaacctgg tgatttctaa aagaagtttt ttgcatggta ttttcagaga
    11881 ttatgaattg ccgcattata gcctaataac gcgcatcttt catgacggca aacaataggg
    11941 tagtattgac aagccaatta caaatcatta acaaaaaatt gctctaaagc atccgtatcg
    12001 caggacgcaa acgcatatgc aacgtggtgg tagacgagca aaccagtagc gctcgaagga
    12061 gaggtgaatg gaaagtaaag tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa
    12121 cggcaaactc aacgttcctg aaaatccgat tatcccttac attgaaggtg atggaatcgg
    12181 tgtagatgta accccagcca tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa
    12241 aggcgagcgt aaaatctcct ggatggaaat ttacaccggt gaaaaatcca cacaggttta
    12301 tggtcaggac gtctggttgc ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc
    12361 cattaaaggc ccgctgacca ctccggttgg tggcggtatt cgctctctga atgttgccct
    12421 gcgccaggaa ctggatctct acatctgcct gcgtccggta cgttactatc agggcactcc
    12481 aagcccggtt aaacaccctg aactgaccga tatggttatc ttccgtgaaa actcggaaga
    12541 catttatgcg ggtatcgaat ggaaagcaga ctctgccgac gctgagaaag tgattaaatt
    12601 cctgcgtgaa gagatgggcg tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat
    12661 taagccgtgt tcggaagaag gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat
    12721 tgctaacgat cgtgactctg tgaccctggt tcacaaaggc aacatcatga agttcaccga
    12781 aggcgcgttt aaagactggg gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat
    12841 cgacggcggc ccgtggctga aagttaaaaa cccgaatacc ggcaaagaga tcgtcattaa
    12901 agacgtgatt gctgatgcat tcctgcaaca gatcctgctg cgtccggctg aatatgatgt
    12961 tatcgcctgt atgaacctga acggtgacta catttctgac gccctggcag cgcaggttgg
    13021 tggtatcggt atcgcccctg gcgcaaacat cggtgacgaa tgcgccctgt ttgaagccac
    13081 ccacggtact gcgccgaaat atgccggaca ggacaaagta aacccaggct ctattattct
    13141 ctccgctgag atgatgctgc gccacatggg ttggaccgaa gcggctgatt taattgttaa
    13201 aggtatggaa ggcgcaatca atgcgaagac cgtaacctat gacttcgaac gtctgatgga
    13261 aggcgctaaa ctgttgaaat g
//
LOCUS       DAZFRG010000131        13202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFRG010000131 DAZFRG010000000
VERSION     DAZFRG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.131"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(6..68)
                     /locus_tag="ACHKUC_003054"
                     /pseudo
     CDS             complement(6..68)
                     /locus_tag="ACHKUC_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(107..1093)
                     /gene="yeiR"
                     /locus_tag="ACHKUC_003055"
     CDS             complement(107..1093)
                     /gene="yeiR"
                     /locus_tag="ACHKUC_003055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO6619270.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1211..2677)
                     /gene="yeiQ"
                     /locus_tag="ACHKUC_003056"
     CDS             complement(1211..2677)
                     /gene="yeiQ"
                     /locus_tag="ACHKUC_003056"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6619271.1"
                     /translation="MNTIASITLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(2900..3472)
                     /gene="yeiP"
                     /locus_tag="ACHKUC_003057"
     CDS             complement(2900..3472)
                     /gene="yeiP"
                     /locus_tag="ACHKUC_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO6619272.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            3627..3881
                     /gene="yeiW"
                     /locus_tag="ACHKUC_003058"
     CDS             3627..3881
                     /gene="yeiW"
                     /locus_tag="ACHKUC_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6619273.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(3878..5059)
                     /gene="setB"
                     /locus_tag="ACHKUC_003059"
     CDS             complement(3878..5059)
                     /gene="setB"
                     /locus_tag="ACHKUC_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO6619274.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLTCTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAELEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            5427..6557
                     /gene="fruB"
                     /locus_tag="ACHKUC_003060"
     CDS             5427..6557
                     /gene="fruB"
                     /locus_tag="ACHKUC_003060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO6619275.1"
                     /translation="MFQLSIQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDALTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            6557..7495
                     /gene="fruK"
                     /locus_tag="ACHKUC_003061"
     CDS             6557..7495
                     /gene="fruK"
                     /locus_tag="ACHKUC_003061"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO6619276.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            7512..9203
                     /gene="fruA"
                     /locus_tag="ACHKUC_003062"
     CDS             7512..9203
                     /gene="fruA"
                     /locus_tag="ACHKUC_003062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO6619277.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            9659..10600
                     /gene="psuK"
                     /locus_tag="ACHKUC_003063"
     CDS             9659..10600
                     /gene="psuK"
                     /locus_tag="ACHKUC_003063"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO6619278.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            10588..11526
                     /gene="psuG"
                     /locus_tag="ACHKUC_003064"
     CDS             10588..11526
                     /gene="psuG"
                     /locus_tag="ACHKUC_003064"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO6619279.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVIALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            11620..12870
                     /gene="psuT"
                     /locus_tag="ACHKUC_003065"
     CDS             11620..12870
                     /gene="psuT"
                     /locus_tag="ACHKUC_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6619280.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12937..>13202)
                     /locus_tag="ACHKUC_003066"
     CDS             complement(12937..>13202)
                     /locus_tag="ACHKUC_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6619281.1"
                     /translation="NQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLGVSYRHLLYV
                     LAQFINDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ"
BASE COUNT         3232 a         3185 c         3515 g         3270 t
ORIGIN      
        1 tactaggaaa gaaacagcgc gaggccgaca atattcaaca acactatttg cggcaaattt
       61 ttaatcatat tttctctaat tatcttactg aaaaacgcag gcaaccttac gcggtagtcg
      121 ctaaacgaag cttcaacagc gcgctctgca aggcattcca gtcagcttcg ctgctggaaa
      181 tcagctcaat acgactgtcc ggtggcgcaa cgttttgcgt ttcaatgtgc aggtcatcgc
      241 cctgacggtt gattcgcacc agcccttcgg gaatacgtag cacgcctttg acgcgttcca
      301 ccggtgcaag tcgcgcccat tccagaatac caatggtgtc gaataccgta tcagcgtcga
      361 atatccagcc acaggcctga tgcccttgtc cgctgttcag actgcgacgc cagcgttgat
      421 gctctggcag gcttaacgct gctaaccctt ttttcacgct atgctgatga gaatgcgcgg
      481 cgctggcggg caactcggct aaattgcgac gcggcaaatc cagaagatga ccgtcaattt
      541 ttccatgctc gctgtgaatt aattgccgat cgccaccatt ttgctgccac cagcgctcta
      601 gcgcttgctc actttcgggc gtcgcacggt cggatttatt ggcgacaata atgtctgcgg
      661 cagccagttg gtcacggaag ttttcattgc tggcgctttt ttcgtccagc aacaggcgtg
      721 gatcgagaat gcacaacgtg gcgcgcagat ctatccacgg ttcatagacg ggtgcggtta
      781 agagatcgag gatctgtttc ggatggccca gcccggtcgg ctctatcaac aagcggtctg
      841 gttttccctg acgcagtaag gtattcaacc ctacctgcat gggtaaacca ttaacgcagc
      901 acatgcaacc gccggggatc tctttcagca gtgcgccgct atcggcgagc aaagcaccat
      961 caattccgac ttccccaaat tcattaacca gtaccgccca tttttcgttg ggatctttat
     1021 gggctaacag atgaagaatc gacgtagttt tcccgctgcc gagaaaaccg gtgatgaggt
     1081 tggttctggt cacaacggct ccgcaatact ctggtaagtt aactgagcgt taatcctggc
     1141 taaaaaaggc agaaaagaga aggggagagc ggacgaaggc gagcttcatc cgctttaacc
     1201 gcagaaatcg ttaaattttg agggtgttta atagcgcctg atgcgcgccg aattgtgcga
     1261 tttgttgcca ggcttgttcg atggcctgca caaaatgcgg gttatctggc agatcatcac
     1321 cgaaaatttc acgcagggaa agcagggcgg ttacgcgttg ttcactgctg ctgcccgcaa
     1381 caagttcacg aattttatcg ctaagcggat cgcgaacatc aatggcattt ccggcatcat
     1441 caacgccgct gacgtaacgc atccagcctg caacgcctaa tgccagcaac gaccagtccg
     1501 tttcgcgccc cagatgtatg cgaatacctg ccagcatgcg ttgcggtaat ttttggctgc
     1561 catccatcgc gatttgccag gtcttatgtt tcagtgccgg attagcaaaa cgtgcaatta
     1621 acttatccgc atattgtgtt aaatcgacat ctttaatccg cagtgtcggc gcttgctcat
     1681 ccagcattaa tgttctggcg gcatggcgaa atgcgcgatc ctgcatgcaa tcactgatat
     1741 gggcgaatcc tgagaggtaa cccagataag cgagaaaaga gtggctgcca ttaagcatac
     1801 gcagtttcat ctcttcccat ggcaggacat cattcaccat ttgtacacct gcgacttccc
     1861 aggcaggacg cccagcgacg aagttatctt caaccaccca ctggataaac ggttcgcagc
     1921 taatcgcgca gggatcattc acccccagat gctggcttat ttccgccagt gattcgttgg
     1981 ttgcagccgg aacaatgcgg tcgaccatgg ttcccggaaa actgacgtgt tcttttatcc
     2041 acccggcgag ttctggtgaa cgtttttccg ccatccccag caccgcgttt ttcaccacat
     2101 ggccattgtc gggaatattg tcgcaggaga gcacggtaaa cggtgtaagg ccgcgctcac
     2161 ggcggcgttt cagtgcttcg acgagaatac ctggcgcgga gtgaggttct tcaggggttt
     2221 gtagatcgtg aataatccgc ggattgctgg tgtcgagtgc accggtggcc gggtcaatac
     2281 aatagccttt ttcggtaatc gtcagggaaa caattgccac ctgtggctcg caaaacttct
     2341 caataattgc cgctaaggaa tccagttttg cattcaggca ttcgtgaacg gcaccgacaa
     2401 ttatcgcctg attgccgtcc gcccctttct ccagcacggt atataaatgg ttctgtgcgc
     2461 ggagctggct catcagttga tcgccgctga acaagctgat ctcacagatc ccccagtcgc
     2521 cgccctgggc attcagcaca cgatcggtca gtaacgcctg atgtgcgcgg tgaaaggctc
     2581 caaagccaaa atgaacgata cgtgattgca actgctgtcg atcgtagcgt ggagcgtgta
     2641 catgatgcgg gagcgtaatg gaggcgattg tattcatagg agtcacgtct tagtaacgat
     2701 taattaacaa tgacagtgta aagttatatg acaacaattg aaattggtct ggattgttaa
     2761 tgcgatgaag tgtgagggcg atcaatcaac gagtgaaaat tgcgggcgta aggtgttcag
     2821 gaagcgcaaa atagaagatt ggtataataa attaaaaaag ggacagcctg agctatccct
     2881 tttctgcaag cggctgaagt tagtcagcac gacccatata acggcgttct tcgatatgga
     2941 tacgaatttt ttcgcccggg cttaagtatt ctggtacctg aatcaccaga ccagtgctca
     3001 atgtcgccgg tttgttacgg gcgctggcag atgccccttt gatgcctggt gcagtttcaa
     3061 cgatttccag atcaacggtc tgcggcagct caagcgccag caattgaccg tcccaggtca
     3121 gcacctgcat gtccggcatg ccgccctcag gcataaacag caactcttct tcaatctgat
     3181 ctttggtgaa ggtatacggg gtatagtctt ctttatccat aaagacatat tcgttgccat
     3241 cgacatagga gaagtcaacg taacggcggg tcagcgtcac ggtgtcaacg atatcatcac
     3301 ctttgaaacg ctcttctact ttcagcccgg tacggacatc agaaaaacgc attttgtaca
     3361 gcgtagcggc accgcgggca gtgggcgact gaatatcaat gtccttaacc agcagcagtt
     3421 tgccgttgta attcagtacc ataccttttt taatttcgtt cgctcttggc atcgaaaaat
     3481 tcctgtagtc aaaaatgtca aaaatatcgc gacaaagtac tcgcgacggg cctttcgggc
     3541 aagtggtatt cgcacttttg ctggtgcaaa aaaggtggta ctgtgcgcgc tcatcaatcc
     3601 ggtggttaac cttaagagaa caacgtatgg aatgccgtcc gggttgcggg gcgtgttgca
     3661 ctgccccttc aatttccagc cctattcccg gtatgccaga tggcaagccc gccaatacgc
     3721 cctgcattca gcttgatgaa cagcaacgct gtaaaatttt cacctcgcca ctgcgaccga
     3781 aagtctgtgc aggcttacaa gccagcgctg aaatgtgcgg taactcgcgt cagcaggcaa
     3841 tgacctggct tatcgatctg gaaatgttga ccgcgcctta aacatcttta attcgcagta
     3901 agcaaaacag agtggcgata atcatcacca tcgcaaacca gaacacagcg tgataattcc
     3961 agatctcggc gacgatgccc gccactgatc ctgcgatgat ccagcccacg cgcgacgtgt
     4021 tggtatagag cgtggtggct gaacctgcct gaccgggcat caaatcctga aaatagagca
     4081 tcccgatgcc gcccagaatg ccaataaaaa tagcatttag cagctgcaag cccaacagaa
     4141 tgacaggtga atgcgccatc agcattcctg cgtaaaaaca gacgccaccc acggcagcaa
     4201 cgcgcattaa gaagcgctta cccagacgtt tggcaaaata tccggcgatc aacatggtcg
     4261 ggatttccag ctcggcggcg gtccccatca tcacaccggc cagtttctcg ggaagatgca
     4321 gttcgttgat aataaatagc ggcatgttga tgatgtacag gctgttcgag ccccacatca
     4381 atgtacaaat gacaaacagc agcaacgtat cgcgacggtt acggcgcggc gcttcaaccg
     4441 tgccggtcgc cagcggaagc tctttttgca tcgacggcaa aaacagccac accatcacgc
     4501 cgcaaacaat aaacgctacc gctgcgctca gatacattac cgtaaagctg aaacccatcg
     4561 ctaaggcata agccagcggt gggccaatga cccatgccag tgaaacctga gcgcgtaaaa
     4621 aagagctgaa catcaccgcc tcacgtccgg ttttgtcagc atgttcacgg gcaagggcaa
     4681 acatttgcgg gttagcggtt gagccaaagc tgctaagaaa gacgccaacg aatagcaaaa
     4741 caaagtagtt gcgattccag gcaaaaaggg tgcaggtcag cacgcctaac aggcagcaaa
     4801 agacaatcag cgatttgcga tcgccgcgct tatcagagcg cccggcgaga aactgactca
     4861 ccagaatccc aatgactgcg ctgccggtaa agaagaatcc caccatcgcc ggacgggcat
     4921 gtacttcatc agtaagaaaa atactgagtg tcggggtttg cagagcgccc gcaataccgg
     4981 tgagaaaggc aacgattaaa aacgccgtcg aggtcaggtc aaacgatttc gcgctggtga
     5041 ctgcggggga gttatgcatg ctggtcgatc actggaagag gaagacgcgg agtttacgct
     5101 gcttgtcaga aaataaacag acagcgaaat caaaagcgcc acgaataatc aaaatgccat
     5161 ttcactgttg ctgattggct gctgacgctg ctgaaatgaa attgctgata acggattttc
     5221 ccatcagcaa ttaggaaaaa tgacaaaaaa ttgtgcagca catcaaactt ttgctcataa
     5281 ctttacggct ttccttgcgt gctgaaacaa aagtattatg ctttcttgaa acgtttcagc
     5341 gcgatcttgt ctttaaccct aagccaggtt ggcgcttttt ttctcataga agctgaatcg
     5401 tttcaattca gcaagagagg agaactatgt tccagttatc catacaggac atccatccgg
     5461 gcgaaaaggc cggagacaaa gaagaggcga ttcgccaggt cgctgcggcg ctggtgcagg
     5521 ccggtaatgt agctgaaggc tacgtcaatg gcatgctggc gcgcgaacag caaacctcaa
     5581 cgttcctcgg caatggtatt gctattccac acggcactac cgacacccgc gatcaggtgc
     5641 tgaaaaccgg cgttcaggta tttcagttcc cggaaggcgt cacctggggt gacggccagg
     5701 tagcgtacgt ggcgatcggt attgctgcca gctcggatga acatctgggc ctgctacgcc
     5761 agctgaccca cgtactgagc gatgattccg ttgctgaaca actgaagtca gcaacaacag
     5821 cagaagaact tcgcgcattg ctgatgggcg aaaagcagag tgagcagctg aagctcgaca
     5881 acgaaatgct gacgctggat atcgtcgcca gcgatctgct gactcttcag gcgctgaacg
     5941 ctgcgcgtct gaaagaggcg ggggcagttg acgccacttt cgtcaccaaa gccatcaatg
     6001 aacaaccgct gaacctcgga cagggtatct ggctgagcga tagcgccgaa ggcaatctgc
     6061 gtagcgcgat tgcggtaagc cgtgcggcaa atgcttttga tgtggacggc gaaacggcag
     6121 ccatgctggt gagtgtggcg atgaatgacg atcagcccat tgcggttctt aagcgtctcg
     6181 ctgatttgtt actcgacaat aaagctgacc gcttgctgaa agcggatgcg gcaacgttgc
     6241 tggcgctgct gaccagcgat gatgcgctga ccgacgacgt gttaagcgcg gagtttgtgg
     6301 tgcgcaatga acacggcctg catgctcgtc caggtaccat gctggtcaat accattaaac
     6361 aatttaacag tgatattacc gtgacaaacc ttgatggcac cggcaaaccg gcaaacggac
     6421 gtagtctgat gaaagttgtg gcacttggcg ttaagaaagg tcatcgccta cgctttaccg
     6481 cccagggggc agatgctgaa caggcgctga aagccattgg cgacgctatc gctgctggtc
     6541 ttggggaggg cgcataatga gcagacgtgt tgctactatc acccttaatc cggcttatga
     6601 ccttgttggt ttctgcccgg aaattgaacg cggcgaagtg aacctggtga aaaccaccgg
     6661 tctgcatgcg gcgggtaaag gcatcaacgt ggccaaagta ttaaaagacc tgggaattga
     6721 tgtcaccgtt ggcggcttcc tcggtaaaga caatcaggat ggttttcagc aactgttcag
     6781 cgagctgggc attgccaacc gtttccaggt tgtacagggg cgcacccgaa ttaacgttaa
     6841 gctgacggaa aaagacggcg aagtgaccga cttcaacttc tcgggttttg aagtcacccc
     6901 cgccgactgg gaacgctttg tgactgattc tctgagctgg ctcggtcagt tcgatatggt
     6961 ctgtgtcagc ggaagcttac cgtcaggcgt cagcccggaa gcgttcaccg actggatgac
     7021 tcgcctgcgt agtcagtgtc cttgcattat ctttgatagt agccgtgaag cgttagtagc
     7081 aggtttgaaa gcggcaccgt ggctggtgaa acctaaccgc cgcgagctgg aaatctgggc
     7141 aggccgtaaa ctgcctgaaa tgaaagatgt gattgaagct gcgcatgcgc tgcgtgaaca
     7201 aggtattgcg catgttgtta tttcactggg tgccgaaggc gcgctttggg ttaatgcctc
     7261 cggcgaatgg atcgccaaac caccgtcagt cgatgtcgta agcaccgttg gcgcagggga
     7321 ttctatggtt ggtggcctga tttatggctt gctgatgcgt gaatccagtg aacacacact
     7381 gcgtctggcg acagctgttg cagccctggc ggtaagtcaa agcaatgtgg gtattaccga
     7441 tcgtccgcag ttggccgcaa tgatggcgcg cgtcgactta caacctttta actgacagca
     7501 ggagaggcat aatgaaaacg ctgctgatta ttgacgctaa tctcggtcag gcacgcgcct
     7561 atatggcgaa gaccctgctg ggcgcggcgg cgcgaaaagc aaaactggaa atcatcgaca
     7621 atccgaacga cgcagaaatg gcgattgttc tcggtgattc catcccgaat gacagcgcgc
     7681 tgaacggtaa aaatgtctgg ctgggtgata tttcccgggc agttgcgcac cctgagctgt
     7741 tcctgagtga agccaaaggc catgcgaaac cttacactgc gccggtcgct gcgacagcac
     7801 cggttgctgc cagcggtccg aaacgcgtag ttgcggtgac tgcttgcccg actggcgtag
     7861 cacacacctt tatggcggct gaagccattg aaaccgaagc gaaaaaacgt ggctggtggg
     7921 tgaaagttga aacccgtggt tctgttggcg cgggcaatgc aatcacccct gaagaagtcg
     7981 cagcagcgga tctggtgatt gtggcggcag atatcgaagt ggatctggcg aaatttgctg
     8041 gtaaaccgat gtatcgcacc tctaccggtc tggcgctgaa gaaaactgcg caggaactgg
     8101 ataaagcggt tgctgaagca acgccgtatg aaccagcggg caaagctcaa acagcgacca
     8161 ctgaaggtaa gaaagagggt gcaggcgctt accgtcactt gctgacgggc gtctcttaca
     8221 tgctgccgat ggttgttgca ggtggtctgt gtatcgcgct ttcttttgct tttggtatcg
     8281 aagcgtttaa agagccgggt acgttggcag cggcgctgat gcagattggt ggtggttcag
     8341 cctttgcgct gatggtgccg gtactggcag gttatattgc cttctccatt gccgatcgtc
     8401 cgggtctcac gccgggcctg attggcggta tgctggcggt tagcaccggt tctggcttca
     8461 ttggtggtat tattgcgggc ttcctggcgg gttacattgc gaagttaatc agtacgcaac
     8521 tgaaactgcc acagagtatg gaggcactga aaccgatcct gatcattccg ctaatttcca
     8581 gtctggtggt cggtctggcg atgatctacc taatcggtaa accggttgct ggcattctcg
     8641 atgggttgac tcactggctg cagaccatgg ggactgcgaa tgcggttctg ctgggggcga
     8701 tcctcggtgg catgatgtgt actgacatgg gcggtccggt aaacaaagca gcgtacgcat
     8761 tcggtgtggg tctgctgagt actcaaacct atggcccgat ggcggcgatt atggcggcag
     8821 gtatggtgcc accgctggca atgggtctgg caacaatggt ggcgcgtcgc aaattcgaca
     8881 aagcgcagca ggaaggtggc aaagccgctc tggtacttgg tctgtgcttt atttcggaag
     8941 gtgcaattcc gtttgctgct cgtgatccga tgcgtgtgct accgtgctgt atcgttggtg
     9001 gggcgctgac tggcgcaatt tcaatggcga ttggtgcgaa actgatggca ccacacggtg
     9061 gtctgtttgt tctgctgatc cctggcgcta ttacgccggt attgggttac ctggtagcaa
     9121 ttattgccgg tacgctggtg gcgggtttgg cctatgcctt cctcaaacgc ccggaaacac
     9181 aaatagtaga aaaaaacgca taatttctct tcatcatcgg ggctgagaca tctctgcccc
     9241 gatgatgaca aacgcaaaat tgcctgatgc gctatgctta tcagacctac atgaaccctg
     9301 caatatattg aatttttgcg cttttgtagg tcggataagg cgtttacgcc acatccagca
     9361 tgaacaaagc gcgcgttgtc aacaatctga gggcagaaac atctctgccc tttttcttgc
     9421 ctgccatatt gtgtgttaca catcacaact ttctcttttg ttgctgcata aatagacaaa
     9481 ctttgtgacc gccttcatat ctggccgatt tcgttttttc tgatcgattt acattacgtt
     9541 aaacatgctt attatgaaca tgtgtttaaa tctgaacacg tgctctaata gtcttattga
     9601 ctgtaatgac tattttaccc aatttcttcg ttggcaattt attgccaggg ggcgtgctat
     9661 gcgcgaaaag gactacatag taattattgg ctcggcgaat attgatgtgg ctgggtattc
     9721 acatcatcca ttgaattatg cggattcgaa tcccggaaaa attaaattta ccccaggtgg
     9781 cgtgggaagg aatatcgctc ataatctggc gttactgggg aaaaatgcct ggcttttaag
     9841 tgcagtaggt ggtgattttt atgggcaatc attactggcg caaactaatc aatccggtgt
     9901 ttatgtcgat aaatgcttaa ttgttccggg tgagaataca tccagctatt tatcactgct
     9961 cgataataca ggagaaatgc ttgtcgcgat aaatgatatg agtattagtg actgtatttc
    10021 tgcagaattt cttgcccagc atcaggagtt tattcgcggt gcaaaggtta ttgtcgcgga
    10081 ttgcaacctt agtgaagagg cgctggtctg ggttctggaa aatagcgggg aaacgcctgt
    10141 atttattgat ccggtatcgg catggaaatg cgtcaaaatc cgcgatcatc tgagcaaaat
    10201 ccatacgtta aaacctaacc gtcttgaagc cgaaacgttg agcggaattg cactttccgg
    10261 acgtgaagat gtcgcaaagg tcgccgcctg gtttcatcaa cacggcctga accgcctggt
    10321 gctgagcatg ggaggggatg gcgtttacta cagtgatatc aacggtgaaa gtgggtggtc
    10381 ggcccctatt aaaacgaatg tcatcaatgt tactggggca ggcgacgcca tgatggcggg
    10441 gctggcctcc tgttgggtag acggtatgcc gtttatcgat tctgttcgat ttgcgcaggg
    10501 atgttcgtca atggcacttg cctgtgaata taccaacaac cctgaattat caattgccaa
    10561 tgttacatcg ttagtggaga acacagaatg tctgaattaa ctctgtcccc tgaattatta
    10621 caaatatccg ctgaagtaca ggatgcttta aaaaataaaa aaccggttat tgcgctggaa
    10681 tcgaccataa tttctcacgg aatgccattc ccacaaaatg cccaaaccgc aattgaagta
    10741 gaagaaacaa ttcgtaaaca gggcgcagta ccagccacta tcgccattat tggcggcgtg
    10801 atgaaagtgg gcttaagtaa agaagaaatt gaattgctgg gccgtgaagg acataacgtg
    10861 acgaaagtta gtcgtcgtga tttacctttt gtcgttgctg cagggaaaaa tggcgcgact
    10921 accgttgcat caaccatgat tattgcagca ctggctggga ttaaagtttt tgcaaccgga
    10981 ggtattggtg gtgtgcatcg cggagcggaa cataccttcg atatttctgc cgatttacag
    11041 gaactggcaa ataccaatgt cactgttgtt tgtgcggggg caaaatctat tctcgattta
    11101 ggattaacaa cagaatattt agaaaccttc ggtgtaccgt taattggtta tcagacaaaa
    11161 gcgctgcctg cgtttttctg ccgaaccagc ccatttgaag tcagcattcg tcttgacagt
    11221 gcccgcgaaa ttgctcgcgc gatggcagtg aagtggcaaa ccggtttgaa cggcggcctg
    11281 gtggtagcga acccgatccc ggaacagttt gcgatgccag aagaaaccat caatgcagca
    11341 attgatcaag ccgttgctga agccgaagaa cagggtgtta ttgggaaaga gagtacaccg
    11401 ttcctgctgg cgcgtgttgc ggagctaacc ggcggtgata gcctgaaatc caatattcaa
    11461 ctggtcttta ataacgccat tctggcgagc gaaattgcca aagaatatca gcgtctcgtg
    11521 ggttaatttc tggtgggcag aaagccgtcg cccgtactca tcaaacatac caaaacctgg
    11581 cgctgacgcc gggtttactg gcatgaaggg aaaatcatta tggatataat gagaagtgtt
    11641 gtggggatgg tggtgttact ggcaatagca tttctgttgt cggtaaataa aaagagcatc
    11701 agcttgcgca cggttggggc tgcgctgctg ctgcaaattg ccattggtgg catcatgctc
    11761 tattttcccc cgggaaaatg ggcagtagag caggcggcat taggcgtcca taaagtgatg
    11821 tcctacagtg acgcaggaag tgcctttatt tttggttcgc tggtcggtcc caaaatggat
    11881 gttctgtttg acggtgcggg ctttatcttc gcctttcgcg tactcccggc gattattttc
    11941 gttaccgcgc ttatcagctt gctgtactac attggcgtaa tggggctgct gattcgtatt
    12001 cttggcagta tttttcagaa agccctcaac atcagcaaaa tcgaatcttt tgtcgcagtc
    12061 accactattt tcctcgggca aaatgagatc ccggcgatcg ttaaaccttt tattgatcgc
    12121 atgaatcgca acgagctatt tacggctatt tgtagcggca tggcgtctat tgcgggttcg
    12181 atgatgattg gctatgccgg aatgggcgtg ccgattgact atttgttagc cgcttcgctg
    12241 atggctattc ctggcggtat cctgtttgca cgtattctta gtccggcgac ggaaccttcg
    12301 caagttacgt ttgaaaatct gtcgtttagc gaaacaccgc caaaaagcat tattgaagct
    12361 gcggcgaacg gtgcaatgac cgggctgaaa atcgccgccg gtgtggcgac ggtggtaatg
    12421 gcatttgtgg cgatcattgc gctgatcaac ggcattattg gtggagttgg cggctggttt
    12481 ggttttgcca atgtctctct ggaaagcatt ttcggctatg tgctggcccc gttggcatgg
    12541 attatggggg tggactggag tgatgcaaat cttgccggaa gtttgattgg gcagaaactg
    12601 gcgattaatg agtttgttgc ttatctcaat ttctcgccct atctgcaaac gagcggcacg
    12661 ctggatgtca agaccattgc cattatctcg ttcgccctct gtgggtttgc aaactttggt
    12721 tctatcggtg ttgtcgttgg agcgttttcg gctatttcgc caaaacgcgc gccggaaatc
    12781 gcccagcttg gtttacgggc gctggcggcg gcgacgcttt ccaacctgat gagcgcgacc
    12841 attgccgggt tctttattgg tttagcttga gtttcattgc cggatgtacg ccttattaag
    12901 cgttgcatcc ggcaatcgca ctattgccaa taatttttac tgcatcatcc cggagaattt
    12961 attctccggg tccatctcca gcgccagtcc tgacaactgc tttctgtttt taatgagata
    13021 ccctttcttg cttttaatta ataaaccgtc attaatgaat tgcgcaagaa catataaaag
    13081 atgtcgataa gaaacgccta aatactctgc cgcttgcgta tgcttttcgt gataaagatc
    13141 accttcctgc gagagtaata taaaagctgc caggcgattc actaacggaa atgattgatt
    13201 ct
//
LOCUS       DAZFRG010000132        13202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFRG010000132 DAZFRG010000000
VERSION     DAZFRG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.132"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..539
                     /locus_tag="ACHKUC_003067"
     CDS             <1..539
                     /locus_tag="ACHKUC_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6619282.1"
                     /translation="ELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVA
                     ATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQR
                     KLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEK
                     SYEACAAELIPDEEGESL"
     gene            548..2647
                     /locus_tag="ACHKUC_003068"
     CDS             548..2647
                     /locus_tag="ACHKUC_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6619283.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRGD
                     SDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSSSPEPVSTPVKKQKWK
                     KTVTDDVNPWLTSGGWL"
     gene            2644..2856
                     /locus_tag="ACHKUC_003069"
     CDS             2644..2856
                     /locus_tag="ACHKUC_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619284.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2856..4364
                     /locus_tag="ACHKUC_003070"
     CDS             2856..4364
                     /locus_tag="ACHKUC_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6619285.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4309..6336
                     /locus_tag="ACHKUC_003071"
     CDS             4309..6336
                     /locus_tag="ACHKUC_003071"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6619286.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRVQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6423..6746
                     /locus_tag="ACHKUC_003072"
     CDS             6423..6746
                     /locus_tag="ACHKUC_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO6619287.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6739..7014
                     /locus_tag="ACHKUC_003073"
     CDS             6739..7014
                     /locus_tag="ACHKUC_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6619288.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7026..7616
                     /locus_tag="ACHKUC_003074"
     CDS             7026..7616
                     /locus_tag="ACHKUC_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6619289.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRKVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRRLPEARFATGHDHQGRPRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7613..8014
                     /locus_tag="ACHKUC_003075"
     CDS             7613..8014
                     /locus_tag="ACHKUC_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6619290.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8025..8768
                     /locus_tag="ACHKUC_003076"
     CDS             8025..8768
                     /locus_tag="ACHKUC_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6619291.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            8829..9215
                     /locus_tag="ACHKUC_003077"
     CDS             8829..9215
                     /locus_tag="ACHKUC_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6619292.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            9224..9553
                     /locus_tag="ACHKUC_003078"
     CDS             9224..9553
                     /locus_tag="ACHKUC_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6619293.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9525..12599
                     /locus_tag="ACHKUC_003079"
     CDS             9525..12599
                     /locus_tag="ACHKUC_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6619294.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKSLDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIATLQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATDRMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NHRYWQQLEIAQGNWKNGAMRAFQNFTADADNAAGTAEQMLTAAFNSAGNALATFCTT
                     GKLNFKSFTASLLSDLAKIMAQMSMMQAVKGIGSAFGWGSAATASVTPNADGGVYQSA
                     DLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMF
                     APQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQSRDGGLFSGGGR"
     gene            12596..12925
                     /locus_tag="ACHKUC_003080"
     CDS             12596..12925
                     /locus_tag="ACHKUC_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6619295.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            12925..>13202
                     /locus_tag="ACHKUC_003081"
     CDS             12925..>13202
                     /locus_tag="ACHKUC_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6619296.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMA"
BASE COUNT         3192 a         3072 c         4027 g         2911 t
ORIGIN      
        1 tggagttgct tatgtcaaat gtgagcggga tcggtgatgc ttattactgg agtgttttta
       61 aaatcgccga ggcctttggg cttcaccggg acacagtaaa aaaacggctc ctcgcggcca
      121 acactcctgt ggctgcgact gtcaggggga accccgttta cgccctgcag catgtcgggc
      181 ccgccctgtt tagtgtgaag catgaggcag cagactctgt tcatgatcca tcccgtatgg
      241 agccgaaaga gagaaaggac tggtaccagt ctgaaaatga aaggatcaag ctggaaaagg
      301 agcagcgaaa actcatccca gttgatgaag tagtcatcgt ctattcgtcc atgagaaagg
      361 ctgtcgtcca ggttctggag acaattccgg atgttcttga acgcgattgc gccctgactc
      421 ctcaggccgt cggcgttgta cagcaggcca ttgatgacct gcgatacact cttcaggaaa
      481 aatcctacga ggcttgtgct gctgaattaa ttcctgatga ggaaggagag agtctctagg
      541 aggaataatg ggtttttcat cagcccgaaa tttgggaagg gacatatcgg caggattttc
      601 cccaccacgt cgcatgccga tttcggaggc tgttaaaaaa ttcatgcgtg ttcccaaggg
      661 ggctggtaac tcggtgccat gggatcctga actgacaccc tacatcattg agcccatgaa
      721 ctgcctggca tcgcgtgaat acgatgcggt gatttttgtt ggtcctgcgc gaacagggaa
      781 gaccattggt ctgatcgatg gatggattgt ctataccatc gtttgcgatc cttcggacat
      841 gctcgttgtg cagatgaccg aagataaggc ccgcgagcat tctaaaaagc gcctcgacag
      901 aacgttcaga agcagtgcgg cggtaaagaa aagaatgagt ccacgtcgta acgacaataa
      961 tgtccatgat aagacgttca gggatggctc gttccttaaa attggttggc cctcggtcaa
     1021 cattatgtcg tcgtcggatt accggtttgt cgccttaacc gattacgacc gttttccgga
     1081 gaatatcgac agcgagggtg atggtttttc cctggcctca aaacgtacca ccacatttat
     1141 gtccgccggg atgactctgg tggagagctc gccgggacgt gacatctgcg acagcaaatg
     1201 gcgacgtaag tcgcctcatg aagcgccacc gacgactggt attctttccc tttacaatcg
     1261 tggtgaccgc cgccgctggt actggccatg tccgcactgc ggtgaatatt ttcagccagc
     1321 tatggatgcc atgaccggct accgtaatga accggatccc tttaaagcta gtgaggcggc
     1381 gtatctactt tgcccgcact gcagcggcat tatcactgcg gagaaaaagc gtgagctcaa
     1441 tagtgcagga gtctggttgc gtgaaggtca ggtcattgat cgtaacggca acgtttccgg
     1501 tgaaccgcgc cgctcccgta tcgccagttt ctggatggaa gggccagctg ctgcgtatca
     1561 gacctgggcg caactggttt acaaattact gactgcagaa caggagtatg aagcgacagg
     1621 aagcgaagaa acactcaggg cggttatcaa caccgactgg ggattacctt atcttccccg
     1681 cgccagcatg gagcaacgaa aaagtgaact gcttgagcag cgggcagagc cagttccttc
     1741 ccgcagtgtg ccagatggcg ttaattttct tgtggcgaca gtggatgtgc aggcgggacg
     1801 tcatcgccgt tttgtggttc aggtaacggg ctatggcagc cgtggcgaac gctggattat
     1861 tgatcgttac aacatcacgc agtcattgcg cggtgacagc gacggggaga gccagcgaat
     1921 tgatccggcc agctatccgg aagactggga tgtcctgctg acggatgttt ttcataaaag
     1981 ctggccgctg gcctccgatc cttctcaaca aatgcgactg atggcaatgg cggtggactc
     2041 cggcggtgaa gacggggtca ctgataatgc ctataaattc tggcgtcgtt gccgtcgtga
     2101 tggccttggt aaacgtattt acctgtttaa gggcgacagc atccggcgcg caaaactgat
     2161 cacccgtaca ttccctgata acaccggacg aacgggccga cgggcgcagg ccgcaggtga
     2221 tgtgccgctc tggcttcttc agacggatgc actgaaagac cgggtgaata acgcgttatg
     2281 gcgtgactcg ccaggtcccg gctatgtgca tttccctgac tggctgggga gctggtttta
     2341 cgacgaactg acgtatgaag agcggagcag tgacgggaaa tggagtaagc cgggtcgcgg
     2401 tgccaacgaa gcttttgacc tgatggtgta tgccgaggct ctggtcattc tgcatggata
     2461 cgaaaagatc cgctggccgg atgcaccgga gtgggcgagc cgggaaacct ggctggagtg
     2521 tgtcccggac agtaccgaac cgtcatcctc accggaaccg gtatccacgc ctgttaaaaa
     2581 acaaaaatgg aagaaaacag taactgacga tgttaacccc tggctgactt ctggaggatg
     2641 gttatgaacc agaatgatat cgaagccatg attcagcgtt atacggaagc tgaaatggcg
     2701 gtgctggacg gaaaatccgt cacctttaat ggtcagcaga tgaccatgga aaacttatct
     2761 gagatccggc agggacggca ggagtgggag cgccgccttg cggctctgat tacacgacga
     2821 cgggggcatc ccgggtaccg gctggcgagg ttctgatggc aattcttgat gatgtgattg
     2881 gcgttttttc accaggatgg aaagcggcaa ggctgcgttc ccgtgcggtg atccaggctt
     2941 atgaggccgt aaaaacgacg cggacacaca aagcccggcg ggagaaccga actgccgacc
     3001 agttaagcca gtacggggcc gtgtcgttac gtgagcaggc ccgttacctt gataacaacc
     3061 acgatctggt tattggtgta tttgacaagc tggaagaacg ggtggtgggg aaaaacggga
     3121 ttattgtcga gccacatccg gtattacgca atggggccat tgcccgtgat ctggcagcgg
     3181 agattcgcac ccgatggagt gaatggtctg tcagcccgga agtcaccggg cagtttaccc
     3241 gtccgatgct ggaacgtctg atgctgcgta cctggctgcg cgatggtgag gtgtttgccc
     3301 agatggtttc cgggcgcata aacagcctga cgccttctgc cggtgttcat ttctggctgg
     3361 aggcgctcga gccagacttt attcccatga ccagtgatga gagcaacagg ctgaatcagg
     3421 gcgtgtttgt tgatgactgg gggcgtcccg aaaaatatct ggtgtataaa agtcgtcctg
     3481 tatccggacg gcagatggaa accaaagaag tggatgcaga gcgaatgctg catcttaaat
     3541 ttgttcgccg tctgcaccag atgcgcggga cgtctttatt gtccggtgtg ctgatccgcc
     3601 tcagtgccct gaaagagtat gaagattctg agctgactgc agcaaggatc gccgctgctc
     3661 tggggatgta catccgcaaa ggcgacgggc agagctatga accggatggt aatggcagca
     3721 aggataagga acgcgagctt accattcagc caggcattat ttacgacgat ctgaaacccg
     3781 gcgaagaaat cggaatggtg aagtcggatc gccccaatcc taaccttgaa acttttcgta
     3841 atggtcagtt gcgtgccgtg gcggcgggca gtcgtctgag tttttccagt acagcgcgca
     3901 actataacgg cacttacagc gcccagcgtc aggagctggt tgaatctact gatggctacc
     3961 tgatcctgca ggactggttt attggtgccg tcacccgccc gatgtatcgt gcctggctga
     4021 aacaggctgt ggcatccggt gttatcaggc taccccgcga tcttgaccgt tcttcactgt
     4081 ataccgcggt gtattccgga ccagtgatgc cgtggattga ccctgttaag gaggctgagg
     4141 cctggaaaat ccagattcgt ggtggagcgg cgacagaatc agattgggta cgtgctggtg
     4201 gtcgtaatcc ggatgatgtc aaacgtcggc gcaaggccga aattgatgaa aaccgcaagc
     4261 tggatctggt atttgatacc gatccggcca gtgataaagg aggcagcagt gccgcaacga
     4321 aacgacagga gccgcagcac accgacgacc agtccgaaga ataattcctg gttcaggatg
     4381 caggctggtc accagagtga cgcggatatt tatatttatg acgagattgg tttctggggt
     4441 gttacagcga agcagtttat cagtgatctg aatgcactgg gcgatatcac ccacattaat
     4501 ctccatatca attcaccggg tggcgatgtc tttgaaggca tagccatttt taatgcgctg
     4561 aaaacacatg gtgcgtccat taccgtttat gtcgacggtg tggcggcgtc aatggcgtcg
     4621 gtcattgcga tggtgggaaa cccggtcatt atgccggaaa acaccttcat gatgattcat
     4681 aaaccatttg gctttacggg cggtgatgcg gaggacatgc gcacctatgc cgacctgctc
     4741 gataaggttg aggcggttct gttacccgct tatgcacaga aaaccgggaa aaccaccgat
     4801 gaaattgctg ccatgctggc ggatgagacc tggatgtccg gtgccgaatg tctggcacat
     4861 ggatttgctg atcaggtgac gccagccgtt aaggcaatgg catgtattca gtcaaaacgt
     4921 acagaggaat ttaaaaagat gccggaatcc attcgaaaca tgattactcc gccacgcaac
     4981 agtgctccac gcgtacagga tgatgaacct gcagcctccc ggacgccagt gcaggcagca
     5041 gcacccgtgg tggatgaaaa cagtatccgt gtgcaggtac tggcagagca aaaagcgcgt
     5101 gtaaacggta ttaatgatct gtttgccatg tttggcgggc gttatcagac gctgcaggct
     5161 cagtgtcttg ccgatcctga atgttcgctg gagcaggccc gcgaaaagct gttgaacgag
     5221 atggggcgcg agtccacgcc atccaataaa aatacaccgg ctcatattta tgccggtaac
     5281 ggtaattttg tgggggacgg gatccgccag gcgctgatgg cgcgtgccgg atttgaaaaa
     5341 accgaacgtg ataatgtcta caacgggatg accctgcgtg aatatgcccg tatgtcactg
     5401 actgaacggg gtattggggt ttccagttat aacccgatgc agatggtcgg tgcggcgttc
     5461 acacacagta cgtctgactt cggtaatatt ctgctggatg ttgcgaacaa agccattctg
     5521 cagggctggg aagatgctcc tgaaacctat gaacagtgga cgcggaaagg tcagttgtct
     5581 gattttaaaa ttgcccatcg tgtgggtatg gggggcttca gtgctctgcg tcaggtgcgt
     5641 gaaggggcgg aatataaata cgtcaccacc ggagataaac aggccactat tgcactggcg
     5701 acctatggcg agctgttcag tatcacccgt caggccatta tcaatgatga tctgaatatg
     5761 ctgaccgatg tcccgatgaa actgggccgt gcggcgaaat ccactattgc cgatctggtt
     5821 tatgccattc tgacgtctaa cccgaaaatc tccacagata atgtaagtct gttcgataaa
     5881 gcgaaacatg caaacgtact ggagagcgct gcaatggacg tggcatcgct ggataaagcc
     5941 cgccagttga tgcgcgttca gaaagagggg gagcgtcatc tgaatattcg tcctgcgttc
     6001 gtactggtac cgacggcgat ggagtctgtt gctaaccagg tcattcgctc ctcaagtgtc
     6061 aagggggctg acattaacgc cggtattatt aacccggtga aagattttgc gaccgttatt
     6121 gcagagcctc gtcttgatga taacagccag accaccttct acctggctgc gtcaaaaggc
     6181 tccgatacga ttgaagtggc ttatctcaac ggtgtggata cgccatatat tgatcagatg
     6241 gagggcttca gtgtggatgg cgtgacaacg aaagtgcgta ttgacgccgg tgtcgcgcca
     6301 gttgatcacc gcggtctggt gaaatgtacg gcgtaaacgt cgcagataac aactctgatg
     6361 gcccgtaagg gctttttttg tacctgaaat cagcccctga acggggctgt gcggagacag
     6421 ttatggcaaa gaattttgta gaagaaggaa aaacggtggc gattgttgcc agtgcagcca
     6481 tcagcagcgg agatctggtg caggtgggtg atgtttttgc ggtggcgctg accgatattc
     6541 cacagggtga aacaggcgac ggcatgaccg aaggtgtgtt tatgctgcct aagctgaaaa
     6601 cggatgatat gaaaacgggt aagaaggttt atctgaagtc cggaaaagtt cagctgacta
     6661 acagcggctc tgatccgctg gtcggggttg tctgggcaga tgccggaacc agtgcagaag
     6721 aagtgccggt aaaactcaat gtctgatccc ttttcccggc tggcagcgcg tatggatgcg
     6781 atcacggtca gaaagatggg aaagacagcc tcgattaatg atgtcgatat gactgtgatc
     6841 ccgggagaaa cactggcaga gctgaatgct ctgtccggac ctgcggtctc tctggtggtg
     6901 ttttcttcgg gataccgccc acggcgcggg gatcgcgttg tttatgacgg acaacaatgg
     6961 acggtcacac ggcatgaacg ctttaacggt aaaccaatga tctttattga gtaaagaggt
     7021 gtgggatgaa ggggcttgag aatgccatcc gcaatctgaa cagccttgat acccgtatgg
     7081 tgccacaggc cagcgcatgg gcgataaacc gtgtggcaca gaaagcggtc tcggttgcca
     7141 cccggaaggt tgccgggaat accgttgcgg gagataacca ggtgaaaggg atccccctga
     7201 aactggtacg tcagcgtgtc cgggtgttta aagccagtcc gtcaggaaaa atgacggcca
     7261 ggatccgcgt taaccggggc aatctgcccg ccatcaaact gaacacaaca cggcggcgtg
     7321 ctggtgaagg actgagagtg ggaaaatact ttttccgggg ggcatttgtt cagcaactgg
     7381 cgaatggccg ctggcatgtt ctgaggcgtc ttcctgaagc gcgttttgca acagggcatg
     7441 accatcaggg caggccaaga aaaaatcgtc ttcctgtgga ggtagtgaaa atcccgctgt
     7501 ccggaccgct gacacaggca tttgaagatg cccgcgaccg catcattgct gcggaaatgc
     7561 cgaaacagct ggggtatgca ctgaaacaac aactgagatt atggctgacc cgatgaaccg
     7621 acatacacaa atccgccagg tcgtactggc acgccttcgg gaacagtgtg gagacagcgc
     7681 cacgtttttt gacgggcttc cggcatttat tgatgcgcag gaactgcctg ccgtggcggt
     7741 gtggctgagt gatgctcagt acaccggaaa aatgacggat gaagatgact ggcaggctgt
     7801 tctgcatatt gctgtcttca tccgggcaca ggcaccggat tcagagctgg atatgtggat
     7861 ggagagcacc attttcccgg ccctgaatga tgtaccggca ctttccggac tcatcgacac
     7921 cctgatccca ctcggtttta actatcaacg tgataatgag atggccacct gggcgatggc
     7981 ggaaatcacg taccagatca cgtacacgaa ttaaggaggt ggcaatgacc acaccaaatc
     8041 cactggcaaa gacgaaaggt gcggggacga cgttctggat gtataccggc aacggcgacg
     8101 catttgcgaa ccctttgtcg gacactgact ggctgcgtct tgcgatggtg aaggatctgc
     8161 aacctggcga aatgaccgct gatgcagaag atgatactta tctcgatgat gaagatgcag
     8221 actggaaaac gacaacccag gggcagaaat cagtcggtga tacttcggcg acgctggcct
     8281 ggcgtccggg tgacagcggg cagaaaaaac tggttcagtt gttcgactcc ggtgaagtct
     8341 gcgcgtttcg tatcaaatat cccaacggta ctgttgatgt tttccgtggc tggctgagtt
     8401 cactgggtaa aaccattgcc tcaaaagacg tgatgacccg cactgtgaaa atcagcggtg
     8461 tggggcgtcc gtatctggca gaggaaggca ctgaaacagt gagcgttacc gggctgacgg
     8521 tggcaccggc atctgccagt gtaaaagtgg gagcaaccac cacgctgacc tttacagtaa
     8581 aacctgacgg agccagtgac aaagcgatca gtgtgcattc gtcagatcca cagactgcca
     8641 cggtgaccct gaacgggctt gtagccacgg tgaaaggcgt gaagcagggc agtgtcagca
     8701 ttgtgggcat gaccgctgac gggaattttg tggctgtggc tgcggtgact gtcagcgccg
     8761 caggttaaca ggacgatact tatcatttgc cccggttatc cggggctttt ttgcaggtgg
     8821 agaacatgat gtttctgaaa cagggcacgt ttaattatga aaaacagtcc gtggtgctca
     8881 gtgagctgtc cgggctgcag agaattgaat atctggcgtt tgttcagcag cgaacggcaa
     8941 agtttgatgc cggtgaggga gaactgccgg aggctgaacg acagattgct tttctgcgga
     9001 tggggatgga tatcaatgcc tggctggttt cccgctcact gtggaatgcg gatcagtcta
     9061 aggatgtaga gacgctttgc gcatccgtta ttacaacatg gtcgtatgat gccctgggtg
     9121 cgggggcgga gatggttctg tcgctgagcg gtatgggggc cattgataat gccggggatg
     9181 atgagcatga ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt ttgtcatgcg
     9241 gcttgcccgg gagttccggc gggcagactg gcggcggatg ctgtcggaaa tgtcggccac
     9301 tgagcttggt gagtggggcg attatttccg gatgcagagc ttcagtgatg tgtggatgga
     9361 tgcgcagttt gcctcgctga aggcattgat cgtgagaatg gtgtccggca gcagtgatgc
     9421 tgcggtggct gatttcagcc ttttaccgga agagaacggg ataccggagc gaacggacga
     9481 agaactgatg catcttgggg aaggtatttc cggaggtgtg cgttatggac cagatagcca
     9541 acctggtcat tgatttgggg attgatgcgg cagagtttaa aaatgaaatt ccccgtatca
     9601 aaaaccttct gaatggtgca gccagcgatg cagaacggtc ttctgcccgt atgcagcgtt
     9661 ttatggagcg tcagactcag gccgcccggc agacaacgca ggcggcttct tcggctgcaa
     9721 cagccgcatc cgttcatgcg cagacggtgg agaagaacgc acaggctcat gaacgcatgg
     9781 cccgtgaggt ggagaaaacc cgccagcgca tggaggcact gagccagaaa atgcgcgagg
     9841 aacaggcgca ggccatggct ctggcggagg ctcaggataa agcggctgct gcgttttatc
     9901 gtcagattga cagtgtgaaa caggccagtg cggggctgca ggaattacag cgtattcagc
     9961 agcagatccg acaggccaga aacagtggcg ggattggtca gcaggattat ctggcgctga
    10021 tttctgaggt tactgcgaaa acccgtgttc ttacacaggc tgaggaagag gctacccgac
    10081 agaaagtggc gtttatccgt cagcttaaag agcaggcaac ccgccagaat ctttcttctt
    10141 ctgagttgct tcgtgctaag gctgcccagc tgggggtaag cagtgctgca gaagtgtata
    10201 tccgcaaaat ggagcaggca ggaaaagcca cgcattcgct ggggctgaaa agtgcagcgg
    10261 cccgtcagga gataggcgtt ctgataggtg aactggcccg cggaaattta ggtgcgctga
    10321 ggggatccgg aataacgctg gctaaccgtg ccgggtggat agacacattg atgtcaccga
    10381 aaggcatgat gcttggcggg gttattggcg gtattgccgc ggctgtctat ggcctgggta
    10441 aagcctggta tgacggtcag aaggaggggg aagaatttaa ccgccagctg tcgctgacgg
    10501 ggcattatgc cggagtcact gccgggcagc tgtggacgct cagtcgtgct atttccggga
    10561 atggtatcac gcaacatgct gcagccggtg cgctggctca ggtggtgggg agtggtgcat
    10621 ttcgtggaaa cgatatcggt atggtggcga gagctgccgc acagatggag cgatcggttg
    10681 gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg
    10741 ccgcgaagtc tctggacaat gagctgcatt ttcttactgc cactcagctt gagcagatac
    10801 gcgtccttgg agagcagggg cggtccagtg atgcggcacg gatagccatg tctgcactgg
    10861 cagaggaaac cggtcggcgt actgcggata ttgataataa cctcaatgcg ctgggcagta
    10921 cgctgcaaac tttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc
    10981 gtgaagattc gctggatgcg cagattgcca ctttgcagga gaaagtgtcg cgggcgaaaa
    11041 gactcccctg gacggcatca tcttctcagg ttgaatacga tcagcagcgt cttaacgatc
    11101 ttcaggagaa aaaacgccag aaggatttgc aggatgcaaa agagcaggca gagcggaatt
    11161 atcaggagca acagaaacgc cgtaatgctg aaaatgctgc actgaaccgg atgaatgaaa
    11221 cggaagctgc acgacatcag cgtgaaattg cgcgtattaa tgccatgcag tacgccgatc
    11281 aggctgtcag ggatgctgcg atacaacgtg aaaatgaacg ttacgagaaa gccctggcat
    11341 ccggtaagaa aaaaacacgc gaaacccgta atgatgaggc cacccggtta ttgctgcagt
    11401 acagccagca acaggcacag gtggaaggac agattgctgc tgcaagacag tcagcaggca
    11461 ttgccactga caggatgaca gaagcgcata aacagcttct ggctctgcag cagcgcatca
    11521 gcgatctgga cgggaaaaaa ctgacggcag atgaaaagag tgtgctggcc cgtaaagatg
    11581 aactgattca ggcactgacg ctgctggatg taaaacagca ggagcttcag aaacagacgg
    11641 cactcaacga tctgaagaaa aaaacaattc agctgaccag tcaactggct gaagaagagc
    11701 gcgctcagcg tcagcaacat gacctggata tcgccacggt gggtatgggt gatcagcagc
    11761 ggcagcgata tcaggtacaa ctgagtcttc gccagaaata ccagcaacag ctggagcagt
    11821 tgaggcggga tagtgagcag aaagggacat ataacacgga tgactacaga aaggccgagc
    11881 aggcgctgac ggagagcctg aaccgacaac tgaatgagaa tcaccgttac tggcaacagc
    11941 ttgaaattgc tcagggtaac tggaaaaacg gtgctatgcg ggcgtttcag aatttcacgg
    12001 cagatgcgga taatgcggca ggcactgctg agcagatgct tacagcggca tttaacagtg
    12061 caggtaatgc actggcaacg ttctgtacta ccggaaaact gaacttcaaa tcttttaccg
    12121 cctcgctcct ttctgatctg gcaaaaatca tggctcagat gtccatgatg caggcagtta
    12181 aggggattgg ttcggcgttt ggctggggga gtgcagcaac tgccagtgtg acgcccaatg
    12241 ctgatggtgg tgtttatcag tctgctgatt tgagtcgcta cagtggcacg gtggttaatc
    12301 gtccgacgtt ttttgctttt gcaaaaggtg caggtgtgat gggggaagct gggcctgaag
    12361 ccattctgcc tctgcgtcgt ggtgctgacg gtaagctggg ggttgtggcg gatattggtg
    12421 gttcaggtat ggcgatgttt gccccgcagt acaacatcga gatcaataac gatggcacga
    12481 acgggcagat aggtccggct gccctgaagg tggtttatga tctcgggaaa aaagcagcag
    12541 cggactttat gcaacagcag tcccgtgatg gtggtctgtt ctccggaggt ggacgatgaa
    12601 aaccttccgc tggaaagtga aacccgggat ggatgtgaca tcggctcctt ccgtcaggga
    12661 ggtgcgcttt ggtgatggct attcccagcg tgcgcctgcc gggctgaacg ctgacctgaa
    12721 aacgtacagc gtgacgctgt ctgtctcccg tgaggaggcc acggcgctgg agtcgtttct
    12781 ggctgagcac gggggctgga aggcctttct gtggacgccg ccttatggct accggcagat
    12841 aaaggtgacc tgcgcaaaat ggtcgtcgcg ggtcagtatg ttgcgtgttg agttcagcgc
    12901 agagtttgaa caggtggtga actgatgcag gatatccgac aggaaacact gaatgaatgc
    12961 acccgtgcgg agcagtctgc cagcgtggta ctctgggaaa tcgacctgac ggtacagggc
    13021 ggtgagcggt attttttctg taatgagctg aatgaaaaag gggagccggt cacctggcag
    13081 gggcgtaagt atgaggcata cccgattgac ggcagcggct ttgagatgaa cggccggggc
    13141 agcagtgccc gcccgtcgct gacggtgtcc aatctgtttg gccttgtcac cgggatggcg
    13201 ga
//
LOCUS       DAZFRG010000133        13059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFRG010000133 DAZFRG010000000
VERSION     DAZFRG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.133"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            146..1255
                     /gene="livK"
                     /locus_tag="ACHKUC_003082"
     CDS             146..1255
                     /gene="livK"
                     /locus_tag="ACHKUC_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO6619297.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1303..2229
                     /gene="livH"
                     /locus_tag="ACHKUC_003083"
     CDS             1303..2229
                     /gene="livH"
                     /locus_tag="ACHKUC_003083"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO6619298.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMISSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2226..3503
                     /gene="livM"
                     /locus_tag="ACHKUC_003084"
     CDS             2226..3503
                     /gene="livM"
                     /locus_tag="ACHKUC_003084"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO6619299.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3500..4267
                     /gene="livG"
                     /locus_tag="ACHKUC_003085"
     CDS             3500..4267
                     /gene="livG"
                     /locus_tag="ACHKUC_003085"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO6619300.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4269..4982
                     /gene="livF"
                     /locus_tag="ACHKUC_003086"
     CDS             4269..4982
                     /gene="livF"
                     /locus_tag="ACHKUC_003086"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO6619301.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5379..6695
                     /gene="ugpB"
                     /locus_tag="ACHKUC_003087"
     CDS             5379..6695
                     /gene="ugpB"
                     /locus_tag="ACHKUC_003087"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO6619302.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTTVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6793..7680
                     /gene="ugpA"
                     /locus_tag="ACHKUC_003088"
     CDS             6793..7680
                     /gene="ugpA"
                     /locus_tag="ACHKUC_003088"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO6619303.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7677..8522
                     /gene="ugpE"
                     /locus_tag="ACHKUC_003089"
     CDS             7677..8522
                     /gene="ugpE"
                     /locus_tag="ACHKUC_003089"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO6619304.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8524..9594
                     /gene="ugpC"
                     /locus_tag="ACHKUC_003090"
     CDS             8524..9594
                     /gene="ugpC"
                     /locus_tag="ACHKUC_003090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO6619305.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            9591..10334
                     /gene="ugpQ"
                     /locus_tag="ACHKUC_003091"
     CDS             9591..10334
                     /gene="ugpQ"
                     /locus_tag="ACHKUC_003091"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6619306.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10321..10761)
                     /locus_tag="ACHKUC_003092"
     CDS             complement(10321..10761)
                     /locus_tag="ACHKUC_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO6619307.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10881..12614
                     /gene="ggt"
                     /locus_tag="ACHKUC_003093"
     CDS             10881..12614
                     /gene="ggt"
                     /locus_tag="ACHKUC_003093"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO6619308.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPFSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(12652..12936)
                     /locus_tag="ACHKUC_003094"
     CDS             complement(12652..12936)
                     /locus_tag="ACHKUC_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO6619309.1"
                     /translation="MITYHDAFAEANNYLDDANLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIVDKGSGEIHSLGTTKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2939 a         3297 c         3719 g         3104 t
ORIGIN      
        1 gggattttaa tcgctatcat cacaaaatac tgtgctaaac ccttaatcag acaggcaaaa
       61 acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataagaaca aagcaacaca
      121 acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat
      181 gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg ccgttgtcgg
      241 cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc
      301 aattaaagac attaatgcca aagggggaat taagggcgat aaactggttg gcgtggaata
      361 tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaacgacgg
      421 cattaaatat gttattggtc atctgtgttc ctcctctacc caacctgcat cagatatcta
      481 tgaagacgaa ggtattctga tgatctcgcc gggagcgacc aatccggagc tgacccaacg
      541 cggttatcaa tacatcatgc gtactgccgg gctggactct tcccaggggc caacggcggc
      601 aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg acaaacaaca
      661 gtatggcgaa gggctggcac gttcggtgca ggacgggctg aaagcggcta acgccaacgt
      721 cgtcttcttc gacggcatta ccgctggaga gaaagatttc tccgcgctga tcgcccgcct
      781 gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa tggggcagat
      841 gctgcgccag gcccgttccg ttggcctgaa aacccagttt atggggccgg aaggtgtcgg
      901 taatgcgtca ctgtcgaaca ttgccggtga tgccgctgaa ggcatgttgg tcaccatgcc
      961 aaaacgctat gaccaggatc cgacaaacca gggcatcgtt gatgcgctga aagccgacaa
     1021 gaaagatccg tccgggccct atgtctggat cacctacgcg gcggtgcaat ctctggcaac
     1081 tgcccttgaa cgtaccggca gcgatgagcc gctggcgctg gtgaaagatt taaaagctaa
     1141 cggtgcaaac accgtaattg ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt
     1201 cgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca agtgatcatc
     1261 ctaccgtccg tgaaaaacgg gcgggtttag aaaggttacc ttatgtctga gcagtttttg
     1321 tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc gctgatagcc
     1381 atcggctaca ccatggttta cggcattatc ggcatgatta acttcgccca cggcgaggtt
     1441 tacatgatta gtagctatgt ctcatttatg atcatcgccg cactgatgat gatgggtatt
     1501 gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat tgccagcgcc
     1561 tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa gcgcctgatt
     1621 gcgcttattt ccgcaatcgg catgtctatc ttcctgcaaa actacgtcag cctgaccgaa
     1681 ggttcgcgcg acgtggcgct gcccagcctg tttaacggtc agtgggtagt gggtcatagc
     1741 gaaaacttct ctgcctctat tactaccatg caggcggtta tctggattgt tactttcctc
     1801 gccatgctgg cactgacgat tttcattcgc tattcccgta tggggcgagc ctgccgtgcc
     1861 tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg ggtgattgcg
     1921 ctgacctttg tgattggcgc ggcgatggcg gcggttgcgg gcgtgctgct cggtcagttc
     1981 tacggcgtaa ttaaccccta catcggcttt atggccggga tgaaagcctt taccgctgcg
     2041 gtgctcggtg ggattggcag cattccgggg gcgatgattg gcgggctgat cctggggatt
     2101 gcggaggcgc tctcttctgc ctatctgagt acggaatata aagatgtggt ctcattcgcc
     2161 ctgctgatcc tggtgctgct ggtgatgccg accggtattc tgggtcgccc ggaggtagag
     2221 aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgctatg ttctttgtgc
     2281 tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg gtggtcgaca
     2341 cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc tttttcttcc
     2401 agcttgtgcg gcctgctttc cagaaagggc taaaaagcgt ttccgggccg aagtttattc
     2461 tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg ctgctggtgc
     2521 ttgcagtggc gtggccgttt atggtgtcgc gtgggacggt ggatatcgcc actctgacca
     2581 tgatctacat tattctcggt ctcgggctga acgtggttgt tggtctttct ggtctgctgg
     2641 tgctgggata cggcggcttt tacgccatcg gcgcttacac ttttgcgctg ctcaatcact
     2701 attacggcct gggcttctgg acctgtctgc cgattgccgg gttaatggcg gcggcggcgg
     2761 gcttcctgct cggttttccg gtgctgcgtc tgcgcggtga ctatctggcg atcgttaccc
     2821 tcgggttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt accggcggac
     2881 cgaacggtat cagtcagatt ccgaaaccga cgctcttcgg actcgagttc agccgtaccg
     2941 cccgcgaagg cggctgggac acgttcagca atttctttgg cctgaaatac gatccctccg
     3001 atcgcgtcat tttcctctat ctggtggcgt tgctgctagt ggtgttaagc ctgtttgtca
     3061 ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt gaagatgaaa
     3121 tcgcttgtcg ttcgctgggc ttaagcccgc gtcgtatcaa gctgactgcc tttaccatca
     3181 gcgctgcgtt tgccggtttt gccggaacgt tgtttgctgc gcgccagggc tttgtcagcc
     3241 ctgaatcctt cacttttgcc gaatcggcgt ttgtgctggc gatagtggtg ctcggcggta
     3301 tgggctcgca atttgcagtg attctggcgg ctgttttgct ggtggtatcg cgcgagttga
     3361 tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg ctgatgatga
     3421 tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actcaagctg aaaaacggcg
     3481 cagcaaaagg agagcaggca tgagtcagcc attattatct gttaacggcc tgatgatgcg
     3541 cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc aggagatcgt
     3601 ctcgttaatc ggccctaacg gtgccggaaa aaccacggtc ttcaactgcc tgaccggatt
     3661 ctataaaccc accggcggca ccattttact gcgcgatcag catctggaag gtttaccggg
     3721 gcagcaaatt gcccgcatgg gcgtggtgcg caccttccag catgtacgtc tgttccgtga
     3781 aatgacggtg attgaaaacc tgctggtggc acagcatcag caactgaaaa ctgggctgtt
     3841 ctccggcctg ttgaaaaccc cctctttccg tcgcgcccag agcgaagcgc tcgaccgcgc
     3901 cgctacctgg cttgagcgca ttggtttgct ggaacatgcc aaccgtcagg cgagtaacct
     3961 ggcctatggt gaccagcgtc gtctggagat tgcccgctgt atggtaacgc agccggagat
     4021 tttaatgctc gacgaaccgg cagcaggtct taacccgaaa gagacgaaag agctggatga
     4081 gctgattgcc gaactgcgta accatcacaa caccactatc ttgttgattg aacacgatat
     4141 gaagctggtg atggggattt cagacagaat ttacgtggtc aatcagggga cgccgctggc
     4201 aaacggtaca ccggagcaga tccgtaataa cccggacgtg atccgtgcct atttaggtga
     4261 ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa
     4321 tccaggcgct gcatgaggtg agcctgcata tcaatcaggg cgagattgtc accctcattg
     4381 gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat ccgcgcgcca
     4441 ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg gcgaaaatca
     4501 tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgtgt cttctcgcgg atgacggtgg
     4561 aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag gagcgcataa
     4621 agtgggtcta tgaactgttt ccacgtctgc atgagcgccg tgttcagcga gcgggcacta
     4681 tgtctggcgg tgaacagcag atgctggcga ttggtcgcgc gctgatgagc aacccgcgtt
     4741 tgttgttgct tgatgagcca tcgctcgggc tggcaccgat tatcatccag caaattttcg
     4801 acaccatcga acaactgcgc gagcagggga tgaccatctt ccttgtcgag cagaacgcca
     4861 accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggtcat gtagtgctct
     4921 ccgataccgg cgacgcgctg ctggcgaatg aagcagtgag aagtgcatat ttaggcgggt
     4981 aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga caaatgtaaa
     5041 attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata ttgaatttgc
     5101 acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg atgagtaatc
     5161 tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc tcccattttc
     5221 cctcgcccgc gccgtcaccg ccttgtcatc tttctgacac cttactatct taaaaatgta
     5281 acaaaatagt tatttttctg taattcgagc atgtcatgtt actccgcgag cataaaacgc
     5341 gtgaattcgc gcattcggta caacaagaga gataatcaat gaaaccgtta cgttatacag
     5401 cttcagcact ggcgctcgga ctggctttaa tggcaaatgc gcaggcagtg acgaccattc
     5461 cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg gcccaacgtt
     5521 ttaacgccga aaacccggat tacaaaattg taccgaccta taaaggcaac tacgaacaga
     5581 atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt ttgcaggttt
     5641 atgaagttgg caccgccacc atgatggcgt cgaaagccat taaaccggtg tatgacgtat
     5701 ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt tcaggctatt
     5761 actccgacag caaaaccggc cacttactct cccagccgtt caacagttcg accaccgttc
     5821 tctattacaa caaagacgcc ttcaagaaag caggattaga cccggaacaa ccgccgaaaa
     5881 cctggcagga tctggcggac tatgccgcaa aactaaaagc ctccggcatg aagtgcggct
     5941 acgccagcgg ctggcaggga tggatccaac tggaaaactt tagcgcctgg aacggtctgc
     6001 cgtttgccag caaaaacaac ggctttgacg gcacagacgc ggtgctggag ttcaacaagc
     6061 cggagcaggt gaaacacatc gccatgctcg aagagatgaa caagaagggc gatttcagct
     6121 acgttgggcg taaggatgaa tccaccgaga agttctataa cggtgattgc gcgatgacga
     6181 ccgcctcttc cggttctctt gccaacattc gcgagtacgc caaatttaac tatggcgtag
     6241 gcatgatgcc ttacgacgcc gatgcgaaag acgcgccgca aaacgccatt atcggcggag
     6301 ccagtctatg ggtaatgcag ggtaaagata aagaaaccta caccggcgtg gcgaagttcc
     6361 tcgacttcct cgcaaaacca gaaaacgctg ccgagtggca tcagaaaacc ggctatctgc
     6421 caatcaccaa agcggcgtat gacctgaccc gtgagcaggg cttttacgag aaaaacccag
     6481 gagcggatat tgccacgcgt cagatgctga acaagccgcc gttgccgttc accaaagggc
     6541 tgcgtctggg caacatgccg cagatccgcg tgattgtgga tgaagagctg gagagcgtgt
     6601 ggaccggtaa gaagacacca cagcaggcgc tggatactgc cgttgagcgt gggaaccagt
     6661 tactgcgccg ctttgagaaa tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg
     6721 cttatctgac ctggcatcgc gtgtaggccg gataagcgaa gcgcatccgg cacagttcag
     6781 gaattaactg taatgtcatc atcccgtccg gtgttccgct cgcgctggct gccttatttg
     6841 ctggtcgcgc cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg
     6901 ttgtggtact cgctacaaag cgtcgatccg tttggtttct ccagccagtt tgtcggcctg
     6961 gataacttcg tcacgctgtt tcacgacagc tactatctcg acgccttctg gacgacgata
     7021 aaattcagca cctttgtcac cgtcagcggt ttgctggtat cgctgttctt cgcagcgctg
     7081 gtggagtaca tcgtgcgcgg cagccgtttc tatcaaacct tgatgttgct accgtatgcc
     7141 gtggctcccg ccgttgccgc ggtattgtgg atcttcctgt ttaaccccgg tcgcgggctg
     7201 attactcatt ttctcgctga gttcggctac gactggaacc acgcgcaaaa cagcggtcag
     7261 gcgatgttcc tggtggtgtt tgcctcagtg tggaagcaaa tcagctacaa ctttctgttc
     7321 ttctatgccg cgctgcaatc catcccccgt tcgttgatcg aagccgcagc cattgatggg
     7381 gctggcccga ttcgccgctt ctttaagatt gcgctgccgc ttatcgctcc ggtgagtttc
     7441 ttcctgctgg tggtgaacct ggtgtatgcc ttcttcgaca ccttcccggt gatcgacgcc
     7501 gccacgtccg gcgggccagt acaggccacc acgacgctga tttataagat ttaccgtgaa
     7561 ggctttaccg gactggattt ggcttcgtct gccgcgcagt cggtggtgtt gatgttcctc
     7621 gtcatcgtgc tgacggtggt gcagttccgc tatgttgaaa gcaaggtacg ttaccaatga
     7681 ttgagaaccg tccgtggctg acaatattca gccataccat gctgatcctc gggatcgcgg
     7741 tgatcctctt cccgctgtac gtggcgtttg tcgcggcgac gctggataaa caagaagtct
     7801 atgccgcacc gatgacgcta atccccggca cacatctgct ggaaaacatc cacaacatct
     7861 gggtaaacgg ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg
     7921 tgatggcgtt cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcatttgcca
     7981 ttgtctggtt tcgttttccg ctacgtaacc tcttcttctg gatgattttt atcaccctga
     8041 tgctgccggt tgaagtgcgt atcttcccga cggtggaagt catcgccaac ctgaaaatgc
     8101 tcgacagcta cgcaggttta acgctgccgc tgatggcctc ggcgaccgcc acgtttctgt
     8161 ttcgccagtt ctttatgacc ctaccggatg aactggtgga agccgcgcgg atcgacggcg
     8221 cgtcgccaat gcgtttcttt tgcgacatcg tttttccgct gtccaaaacc aatctggcgg
     8281 cgctgtttgt gatcaccttt atttacggct ggaaccagta tttgtggccg ttgttgatta
     8341 ttaccgacgt tgatctcggc accaccgtgg cagggattaa agggatgatc gccacaggcg
     8401 aaggcacaac ggaatggaac tcggtgatgg cggcgatgtt gttaaccctg atcccaccag
     8461 tagtgattgt tttagtgatg cagcgtgcct tcgtgcgcgg cctggtcgat agtgagaaat
     8521 aagatggcag gactgaaatt acaggcagta accaaaagct gggatggcaa aacccaggtc
     8581 attaaaccgc tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct
     8641 ggctgcggga aatcgacgtt attgcgaatg gttgccggac tggagcgggt aacgacgggc
     8701 gatatctgga tcgaccgcaa gcgtgtcacc gaaatggagc cgaaagatcg cgggattgcg
     8761 atggtgttcc agaactacgc gctctatccg catatgagcg tagaggagaa catggcgtgg
     8821 gggctgaaaa ttcgcggcat gggcaagcaa caaattgccg aacgtgttaa agaagcggcg
     8881 cgcattctgg agctggacgg cctgcttaag cgccgcccgc gtgagctatc cggcggtcag
     8941 cgtcagcgtg tggcgatggg gcgtgcgatt gtgcgtgagc cggcagtgtt cctgtttgat
     9001 gagccgctct ctaacctcga tgccaagttg cgtgtgcaga tgcgtcttga actgcaacag
     9061 ctgcaccgtc gcctgaaaac gacttcactc tacgttaccc acgatcaggt tgaggcgatg
     9121 acgctcgccc agcgagtaat ggtgatgaac ggcggcgtcg ccgaacagat tggcacgcca
     9181 gttgaagtgt acgaaaagcc cgccagcctg tttgtggcga gttttatcgg cagcccggcg
     9241 atgaacctac tggctggccg cgtgaataac gaaggtacgc atttcgaact ggatggtggt
     9301 attacgctgc cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat gaccctcggc
     9361 attcgcccgg aacatatagc gctaagctcg caggcagaag gtggtgtgcc gctggtgatg
     9421 gacacgctgg agatcctcgg cgcagataac ctggcgcacg gacgttgggg cgaacagaag
     9481 ctggtggtgc gactggcaca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat
     9541 ctgccggaaa atcagctgca tctttttgat ggtgaaacag gacaacgagt atgagtaact
     9601 ggccttatcc ccgcatcgtc gcccatcgtg gtggcggtaa gctggccccg gagaacaccc
     9661 tggcggcaat cgacgtcggg gcaaaatacg gtcataagat gatcgaattt gacgcgaagt
     9721 tatcgaaaga tggcgagatc tttctgctcc atgacgacaa tctcgaacgt accagcaacg
     9781 gttggggcgt tgcgggcgag ctgaactggc aggatttact gcgcgtggat gcaggcagtt
     9841 ggtacagcaa agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg gcggaacgct
     9901 gccgcgaaca cgggatgatg gcgaatatcg aaatcaaacc caccactggc actggaccat
     9961 taacgggcaa aacggtggcg ttggcggcac gcgaactgtg gaccggtatg acgccgccgc
    10021 tgctgtcatc gtttgagatt gatgctttag aagccgcgca acaggcagca ccggaactgc
    10081 cgcgtggttt attgcttgat gaatggcgcg acgactggcg agaattgacc gcgcgactgg
    10141 gctgcgtctc tattcatctc aatcataagt tgctcgataa agcgcgagtg atgcagttga
    10201 aagatgccgg attacggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt
    10261 tgctgcgctg gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact
    10321 ttacggccca atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat
    10381 gtcgccgttt ctcctctccg gcagcatatg ctgctgattc aacgaactat ccggattacg
    10441 gttgctgttt aacatcccgc cgttggtgtt gggcagcatt tgctgccggg cgggatttcg
    10501 ctcccccggc tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg
    10561 ctgctgttgc agctgcgttt gcgttttcag ttgctggttc agcatccctt tttgctggat
    10621 ttgctgcgtc tgcatttgag tttgcatccg ctgctggctg gggatctgat atcccggctg
    10681 gttagggttg ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt
    10741 caaaagcaga agtcgtttca tcgcgtatcc tcctctgaag atattcttta agtttactcg
    10801 cttcccggca aaacgatgat taattcagag ttatatacca ggcttagctg gggttgcccc
    10861 ttaatctctg gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg
    10921 ctctgctcac aggaagttgt tttagtacgg tagccgcgcc acctgtttct tatggtgtgg
    10981 aggaagatgt ctttcatccg gtacgcgcta agcagggaat ggtggcgtct gtagatgcta
    11041 ctgccactca ggtgggggtg gatatcctca aggagggcgg gaatgccgtt gatgccgccg
    11101 tggcggtggg ctacgcgctg gcagtaacgc atccgcaggc agggaatctg ggcggcggtg
    11161 gttttatgct gatccgctcg aaaaatggca ataccacggc tatcgatttc cgcgaaatgg
    11221 cacccgccaa agcgacccgc gatatgttcc tcgatgatca gggcaacccg gacagcaaaa
    11281 aatcactcac ttcgcatctg gcttccggca cacccggtac ggtagcgggt ttctcgttgg
    11341 cgctggataa atacggcacc atgccgttga ataaagtggt gcaaccggcg tttaaactgg
    11401 cacgcgatgg ttttatcgtt aacgacgcgc tggctgacga tctcaaaacc tacggcagtg
    11461 aagtgctgcc gaaccacgaa aacagcaaag ctatcttctg gaaagagggc gaaccgctga
    11521 aaaagggcga caagctggtg caggcgaacc tggcaaagag cctggagatg attgccgaaa
    11581 acggcccgga tgaattctac aaaggcacga tagcggaaca gattgcccag gagatgcaga
    11641 aaaacggtgg cttgatcagc aaagaagatt tagcggccta taaagcggtc gaacgcacac
    11701 cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca tcttccggcg
    11761 gtattcatat cgtacaaatc ctcaatattc tggaaaactt cgatatgcag aagtacggct
    11821 ttggcagcgc cgacgcgatg caaatcatgg cagaagctga gaaatacgcc tatgccgacc
    11881 gctcggaata tcttggcgac ccggattttg tcaaagtacc gtggcaggcg ctgaccaata
    11941 aagcctatgc caaatctatt gccgatcaaa ttgatatcaa taaagcgaag ccattcagcg
    12001 aaattcgtcc tggcaagctt gcgccttatg agagtaatca aactacccat tactcagtag
    12061 tggataaaga cggtaacgcc gtggcggtga cctatacgct gaacaccacc ttcggtacgg
    12121 gcattgtcgc gggcgagagc ggtattttgc tcaataacca gatggatgat ttctccgcca
    12181 aaccaggcgt accgaacgtt tacgggctgg tgggcggcga tgccaacgcc gtcgggccga
    12241 ataaacgccc tctgtcgtcg atgtcaccaa ccattgtggt caaggatggt aaaacctggc
    12301 tggtcacagg aagcccaggc ggtagccgga ttatcaccac tgtgctgcaa atggtggtga
    12361 atagcatcga ttatggaatg aacgtcgccg aagcgaccaa tgcgccgcgt ttccaccatc
    12421 agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg atcaagctgc
    12481 tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca caaagcatta
    12541 tggttgggcc ggacggtgag ctgtacggtg catccgaccc gcgctcggtg gatgatttaa
    12601 cggcggggta ttaaggttag cggccctctt agtgggaaga gggccgtatt gtcagggcaa
    12661 gccgaaggta gcctttttta tttcataatc ctgtagatat tcttccagtg gctttgtcgt
    12721 tcccagagaa tgaatttcac cactgccttt atcaacaata aaaggtgcgt taccagctaa
    12781 gcgcgcggcc tcatctccag tttcgagaaa ttctcgtgct tcgaaacaga aataccagcc
    12841 ctggctaaag cgtccatgta gagtaatgac gaccgggaga tttgcatcat cgaggtaatt
    12901 gttcgcttcc gcgaatgcgt cgtgataagt aatcataatt ataataaata ttattgttta
    12961 gcgttatatt attattcctg cgttatacat tgagcaaaga cgcgttcatc ttcgtcaaag
    13021 atattattaa atgctggttt tgaaaagagt tcatcggat
//
LOCUS       DAZFRG010000134        13022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFRG010000134 DAZFRG010000000
VERSION     DAZFRG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.134"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..227
                     /locus_tag="ACHKUC_003095"
     CDS             <1..227
                     /locus_tag="ACHKUC_003095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619310.1"
                     /translation="HWDLCRIPEDPHHWNGFSGEKRILYGQSAHHFFFSSSTIHVSLP
                     RLITDYEMQKYLSFQLPVIRYRERFWQYME"
     gene            234..2657
                     /gene="pgaA"
                     /locus_tag="ACHKUC_003096"
     CDS             234..2657
                     /gene="pgaA"
                     /locus_tag="ACHKUC_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO6619311.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQTAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2666..4684
                     /gene="pgaB"
                     /locus_tag="ACHKUC_003097"
     CDS             2666..4684
                     /gene="pgaB"
                     /locus_tag="ACHKUC_003097"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO6619312.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHQGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVAVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4677..6002
                     /gene="pgaC"
                     /locus_tag="ACHKUC_003098"
     CDS             4677..6002
                     /gene="pgaC"
                     /locus_tag="ACHKUC_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO6619313.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWSWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDHMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6004..6417
                     /gene="pgaD"
                     /locus_tag="ACHKUC_003099"
     CDS             6004..6417
                     /gene="pgaD"
                     /locus_tag="ACHKUC_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO6619314.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6467..7255)
                     /gene="phoH"
                     /locus_tag="ACHKUC_003100"
     CDS             complement(6467..7255)
                     /gene="phoH"
                     /locus_tag="ACHKUC_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO6619315.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7882..9153)
                     /gene="efeB"
                     /locus_tag="ACHKUC_003101"
     CDS             complement(7882..9153)
                     /gene="efeB"
                     /locus_tag="ACHKUC_003101"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO6619316.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMLHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9159..10286)
                     /gene="efeO"
                     /locus_tag="ACHKUC_003102"
     CDS             complement(9159..10286)
                     /gene="efeO"
                     /locus_tag="ACHKUC_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO6619317.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKSADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10344..11174)
                     /gene="efeU"
                     /locus_tag="ACHKUC_003103"
     CDS             complement(10344..11174)
                     /gene="efeU"
                     /locus_tag="ACHKUC_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO6619318.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            complement(11394..12902)
                     /gene="putP"
                     /locus_tag="ACHKUC_003104"
     CDS             complement(11394..12902)
                     /gene="putP"
                     /locus_tag="ACHKUC_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO6619319.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT         3449 a         3296 c         3047 g         3230 t
ORIGIN      
        1 ggcattggga tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg
       61 agaaaaggat tttatatgga caatctgctc atcatttctt cttctcatca tcaacaattc
      121 acgtgtctct tccgcgttta ataacggatt atgagatgca aaaatatctt tcttttcagt
      181 tacctgtaat tagatataga gagagatttt ggcaatacat ggagtaatac aggatgtatt
      241 caagtagcag aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat
      301 ttttagcagc gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta
      361 ttgaagctcg caaaggaaat actcagccag ctttgtcatg gtttgcacaa aaatcaacac
      421 tcagtaataa ccaaattgct gactggttac aaattgcctt atgggccggg caagataaac
      481 aggttattac cgtttacagc cgctaccgtc atcagcaatt accagcgcgt ggttatgcag
      541 ctgtcgccgt cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa
      601 aggcgctctc tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg
      661 cagatgcggg tcactatgat actgcgctgg ttaaacttaa gcagcttaaa tctggagcgc
      721 cggacaaagc caatttactc gcagaagcct atatctataa actgacgggg cgtcatcagg
      781 atgaattacg ggcgatgaca gagtcattac ctgaaaatgc atctaagcaa caatatccca
      841 cagaatacgt gcaggcatta cgtaataatc aactagctgc cgcgattgac gatgccaatt
      901 taacgccaga tattcgcgct gatattcatg ccgaactgat cagactgtcg tttatgccta
      961 cgcgcagtga aagtgaacgt tatgccattg ccgatcgcgc cctcgcccaa tacgctgcat
     1021 tagaaattct gtggcacgat aactcagacc gcactgccca gtaccagcgt attcaggttg
     1081 atcatcttgg cgcgttatta actcgcgatc gttataaaga cgttatttct cactatcagc
     1141 gtttaaaaaa tacgaggcaa attattccgc cctgggcgca atattgggtt gcgtcggctt
     1201 atctcaaaga acagcagcca aaaaaagcac agtcaataat gacagagctc ttttatcaca
     1261 aggagaccat tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc
     1321 tggagagtga aaattatccc ggcgcgctaa ctgtcaccca acataccatt aatacttcgc
     1381 cgcctttcct tcggttaatg ggcacgccta cgagcatccc gaatgatacc tggttacagg
     1441 ggcattcgtt tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga
     1501 tagccagaga gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga
     1561 gtgtgttaca agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag
     1621 tgatcgagcc acgtaatatt aatctggagg ttgaacaagc atggacagca ttaacgttac
     1681 aagaatggca gcagacagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc
     1741 ccggcgttgt acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag
     1801 ctggttcaac aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa
     1861 ccaccatcgt ttattcacca ccgctgaagg ataactggcg cggttttgct ggattcggtt
     1921 atgccgatgg acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg
     1981 agtggcggtc acgtaatatc tggctcgagg cggagtacgc tgaacgcgtt ttcaatcatg
     2041 agcataaacc cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg
     2101 gttcgcaact ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta
     2161 caggcaacag tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg
     2221 tctcctgggc tttcactgat ttttccgaca gtaaccagcg tcaggaagtc tcacttgagg
     2281 gtcaggaacg catctggtct tcaccatatt tgattgtcga tttcctaccc aatctgtatt
     2341 acgaacaaaa tacagaacac gacactccat actacaaccc tataaaaacg ttcgatattg
     2401 ttccggcatt tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa
     2461 tcttcagcgc aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc
     2521 aactcggcta cgggcaacgt attagctgga atgacgtgat tgatgctggc gcaacgctac
     2581 gctgggaaaa acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata
     2641 tgacattcag attttaagga taaatatgtt acgtaatgga aataaatata tcctgatgct
     2701 ggtgagtata attatgctca ctgcgtgcat tagccagtca agaacatcat ttataccgcc
     2761 acaggatcgc gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat
     2821 ttcatggcat aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc
     2881 agcactgcgt gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc
     2941 tcaaattcgt gaagcacatc aaggaggaaa accgctaccg gaaaaagcag tagtgctgac
     3001 ttttgatgac ggctaccaga gtttttatac ccgtgtcttc ccaattcttc aggccttcca
     3061 gtggccggct gtatgggctc ccgtcggcag ttgggtcgat actccagcgg ataaacaagt
     3121 gaaatttggt gatgagatgg tcagtcgaga atattttgcc acgtggcaac aagtgcgaga
     3181 agttgcgcgt tcccggctcg ttgcggtcgc ttctcataca tgggattctc actacggtat
     3241 tcaggctaat gccaccggca gcttattacc tgcatatgta aatcgtgcat attttactga
     3301 ccatgcacgg tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa
     3361 aatgacggaa tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta
     3421 tggcgaagcg aatggcatag caatcgcgga attaaaaaaa ctcggttatg acatgttctt
     3481 cacccttgaa tcaggtttgg caaatgcgtc gcaattagat tccattccgc gagtattaat
     3541 cgccaataat ccctcattaa aagagtttgc ccagcaaatt attaccgtac aggaaaaacc
     3601 actacaacgg gtaatgcata tcgatcttga ttacatttat gacgaaaacc gccagcaaat
     3661 ggatcgcaat attgatgtgc taattcagcg ggtaaaagat atgcaaatat ctaccgtgta
     3721 tttacaagca tttgctgatc ccgatggtga tgggctggtt aaagaggtct ggtttccaaa
     3781 tcgtttgcta ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tgcgtacccg
     3841 ctcaggtgta aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt
     3901 aacgcgagta aaatacttac caactgggga gaaaaaagca caaattcatc ctgaacaata
     3961 ccgccgtctc tctcctttcg atgacagggt cagagcacaa gttggcatgt tatatgatga
     4021 tcttgccgga catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga
     4081 ttatgaagat gccagtgcag cggctatcgc ggcttatcag caagctggct ttagcgggag
     4141 tctgagcgaa attcgacaaa acccggagca atttaaacag tggacccgct ttaaaagtcg
     4201 tgcgttaact gacttcactt tagaacttag tgcgcgcgta aaagccattc gtggtccaca
     4261 tgttaaaact gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg
     4321 gtttgcacag aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat
     4381 gccttatatg gaaggcgtca cagaaaaatc ggctgatcaa tggttaacac aattgaccaa
     4441 tcagatgaaa agcatcccgc aggctaaaga caaatcaatt ttagaattac aggcacaaaa
     4501 ctggcagaaa aatggtcagc atcaggctat ttcttcacaa caactcgctc actggatgag
     4561 cctattacaa ctgaatggag tgaaaaatta tggttattat cccgacaatt ttctgcataa
     4621 ccaacctgaa atagacgtta ttcgtcctga gttttcaaca gcctggtatc cgaaaaatga
     4681 ttaatcgcat cgtatcgttt tttatattat gtctggtgtt atgcatacct ctatgcgtag
     4741 cgtactttca ctctggtgaa ctgatgatga ggttcgtttt cttctggccg ttttttatgt
     4801 ccattatgtg gattattggc ggcgtctatt tctgggtcta tcgtgaacgc cactggtcgt
     4861 ggggagaaaa cgcaccagcc ccccagttga aagataatcc atctatctcc attatcattc
     4921 cctgttttaa tgaggagaaa aacgttgagg aaactataca tgccgcttta gcacagcgtt
     4981 atgagaacat tgaagttatt gccgtaaatg atggttcaac agacaaaacc cgtgccatcc
     5041 tggatcacat ggctgcacaa attccccgtc tgcgggtcat tcatctggcg caaaaccagg
     5101 ggaaagccat tgcgcttaaa accggagctg ccgcggcgaa aagtgaatat ctggtgtgca
     5161 ttgatggcga cgcgttatta gaccgcgatg cagcggcata tattgtggaa ccgatgttgt
     5221 acaacccacg tgtgggtgcc gtaaccggta atcctcgtat tcgcacacgt tctaccctgg
     5281 tgggtaaaat tcaggttggc gagtattcct caattattgg tttgatcaag cggacccagc
     5341 gtatctatgg aaacgtattt accgtttccg gtgttattgc cgcatttcgt cgcagcgccc
     5401 tggcagaagt gggttactgg agtgacgata tgatcaccga agatattgat attagctgga
     5461 agctgcagtt gaatcagtgg acgatttttt acgagccacg ggcgctgtgc tggatattaa
     5521 tgcctgaaac gttaaaaggg ctgtggaaac agcgcctgcg ctgggctcag ggcggtgcag
     5581 aagtattcct caaaaatatg acaaggttgt ggcgaaaaga aaactttcga atgtggccac
     5641 tgttttttga atactgcctg acgacaatat gggcctttac ctgcctggtc ggtttcatta
     5701 tttacgcagt ccaacttgca ggtgtaccgt taaatattga attgacacat atcgcagcga
     5761 cacatactgc cggaatatta ttgtgcacgt tatgtttact gcaatttatt gtcagtctgg
     5821 taattgagaa tcgctatgag cacaatctgg cttcatcgct tttctggatt atttggtttc
     5881 cggttatttt ctggatgcta agcctggcaa cgacattggt atcatttaca cgtgtcatgt
     5941 tgatgcctaa aaaacaacgc gctcgttggg taagtcccga tcgtgggatt ctgagaggtt
     6001 aatatgaaca atctaattat tacgacccga caatcgccag tacgtttact ggttgattat
     6061 gttgccacaa ccatcttgtg gacattattt gctttgttca tattcttatt cgccattgat
     6121 ctgctgacgg gttattactg gcaaagcgag gccagaagcc gacttcagtt ctattttttg
     6181 ctggcagtgg cgaatgccgt cgtgttaatc gtctgggcat tgtacaataa gctgcgtttt
     6241 aaaaaacagc agcatcatgc agcataccaa tatacgccgc aagaatatgc agagagctta
     6301 gcaatacctg atgagcttta tcagcaacta caaaaaagcc acaggatgag cgtacacttc
     6361 accagtcagg gacaaataaa aatggtggtt tcagaaaaaa cgctagtccg ggtataaaca
     6421 cccaaaacaa agcccggttc gcccgggctc tgcaccgata atacacttaa ctgtatgcat
     6481 gtagcgtacg ttggcaaagt gccgaacgta cgcaatcctc tttaccgaat cggacgatcc
     6541 cgaccatttc atcctcttcg aaacgttcca gcgcgtcact taatccggag cgcacgccgc
     6601 gaggcaaatc gcattgcgtg atatcaccgt tgacgataac cgtcacgttc tccccgaggc
     6661 gggttaaaaa cattttcatt tgcgcggcag tcacattctg cgcctcgtca agaatgacga
     6721 ctgcattttc aaaggtacgt ccacgcatat aggcgaacgg cgcaatttcc accttcccta
     6781 tttccggtcg caggcagtac tgcataaagg aagcccctaa gcgacggacc agcacgtcgt
     6841 agaccgggcg aaaataggga gcaaactttt ctgcgatatc accaggtaag aaaccaagat
     6901 cttcatcggc ttgcagaact ggacgggtaa cgataatcct gtcgacatcc ttatgtatca
     6961 gggcctctgc cgcttttgct gcgctgatcc aggtttttcc gcacccggct tcgcccgtgg
     7021 caaatatcag ctgcttactc tcaatagcct tcagatagtg caattgcgct tcatttcgcg
     7081 cgaggatggg cgaggtatcg cgactgtcgc gggccatacc aatggcttct acgccgctca
     7141 tctgcacaag cgaggtgacc gattcttctt cacgctgctt atggctgcgc gaatcccgtc
     7201 tcagcacacg ttttgcctcg cgacgagctt tgatcactgc tttttgtctt cccatggaga
     7261 gcaccttgag ttgtttgtat tcatcacacg cgccgttggc agcgcgatta tgcgcacgaa
     7321 catcagaggg ttggcttcct tgtaagccat agtttgcttt tggataaaat gccgaaaaac
     7381 ggctacgcgc accgtttacg gcgtcggtaa cacatgaaaa gaaaggatga ggttgaaaat
     7441 acaaagtgac gagatgacta ccggaggaga aatctccgcg agtggtggcg cgctgattat
     7501 ctaaaacatg tccagtacag gacgttacca tccgcgatct ccatagtgac tgactatcac
     7561 tgccgggaac ttccgctgct acttaataag tacaacagat ttcgcattca ttgcaacaat
     7621 atattcactt atatttaact ataaaacacc atttcagtga cattagtttc tactggaaag
     7681 atgacagagt gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat tcgcagtact
     7741 ggtaaaagct tgccggatgc ggcgtgaacg ccttatccgg cctaccgacc cgcaccgatt
     7801 gtaggcatga taagacgcgc aagcgtcgca tcagtgtacg aacgcctggt gcggcgtaaa
     7861 cgccttatcc gcctaaaaac attaaacccg caataatgcg cttccgagat aatcgttcgc
     7921 gtccttcaca cccggcagcg caaagaaata gccgccaccg ataggtttga catattcctc
     7981 cagcgcctcg ccattcaacc ttttttgtac tgtcaggaag cccttttcca gatcgtgttg
     8041 gtagcagaca aacagtagcc ccatatcgag ttgcccggaa ttggtgacgc ccaacgaata
     8101 actgtagcca cgacgcagca tcaggctgga ctcgctctcc gccgtgcggg gattcgccag
     8161 ccggatatgg ctgtccagcg caatcacctt cccttccgga tcgctagcgt aatcgggaac
     8221 atcatgctca tgcagcatgc ccagcggcgc accggtttgc ttatcacgac caaatatcgt
     8281 ctgctgctct ttcagcggcg ttctgtccca aaattccact cgaaactgaa tcaagcgtac
     8341 tgcctgatag ctgccaccga ttgtccacgc aggctcctgc tgatctgccg tcacccacac
     8401 cactttttgc atcaacttcg cattctggct atcgggattg gcggtgccgt ctttaaagcc
     8461 cagcaaattt atcggcgtct ctttgccttt actacgcgct gcgtggtcgg aaataaaccc
     8521 ttcccgcttc cagcgcacgc tgagcaaatc cggcgtgtgt ttgatgatat cgcgcagcgc
     8581 atggatcacc gtgtcctggg tgttggcgca aatttgtagc aacacatcac catgacataa
     8641 cgccgcatcc agcgagtcgt tagggaaacg cgtcatcttc tgcaactttt ttggcatctg
     8701 tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc accgataacg tgatggtgag
     8761 attatcgggc gcaatgtagc cgccaagaat gccggaatcg agtggtggca ggcgcggatt
     8821 tggcgtttct ggtgccgctc cgccctgagt cagaaaagca aaacgctgag tcaacaagcg
     8881 aaacaaccgt tcaagatcgg ctttatcgct ggcaagcaca tcaaacgcca ccagcatcat
     8941 tgcagcctgt tgtggtgtca ggatccctgc ctgatgctca ccataaaacg gctgtttctc
     9001 attgcgtgca tccggtgaaa gcgtccccgg cgcactttgc gttttttgtg catgagcgac
     9061 cggacaactt cccgccagcg ccagcgcacc aatccctttc agtaaacgtc ggcgtgacgg
     9121 ttcattcacg ccgttttcat cttcatactg cataacgctt agtccagccc cagcacaccg
     9181 cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc ctttcagtgc attccggtca
     9241 gcatcggtca gtttgtcgta attttcaaaa ccgtctttag tacggtattt cgccagaatg
     9301 gtatcgacct ttttgaagtt ggcatcgact tttgccaaca gttccggatt cgctttttgt
     9361 agttgtggac gcagcagatc gacaattttc tgcgagcctt caacgttagc ctggaaatcc
     9421 cacagatcgg tgtggctgta gcgatcttct tcaccgctga ttttgctggc tgccacttcc
     9481 tcaatcagtc cggctgcacc gccgaccact tttgaaggag ggaaagccag ttcactgatg
     9541 cgtttttgca aatcgaccac atcggtataa agctggtcag catacttatc catccctttg
     9601 gtggtgttat cgccaaacaa tgctttttcc agacggtgga aaccggtgaa tttcggatcg
     9661 gcggattttt gctcgtaatc atcttcacgg gcgtcaatgc tgccatccag atcggagaac
     9721 agttcagcaa tcggttcgat gcgctcatag tgctggcgcg tcggcgcata cagtgctttc
     9781 gctttttcga tatcgcctgc tttaatagcg tcggtaaagg ctttggtgtc ggtcaccagt
     9841 tgcgtggttt ccgccatgac atacgcttta tatgcagtaa ttgcaccacc aagacttaac
     9901 agcgcatcac tttgcgccgc atccgccgtt gcttcacctt tgacgatcaa cttccctttc
     9961 gggttagtca gcagaccgca ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt
    10021 ttctggctaa aaccaggggc gatattttcc cgctcttcca ccaccatcac gcctttgagg
    10081 atctcccact ccagcgcctt ctggctgtgg ttctgaataa tgaactgtgt tttcccggcg
    10141 ttaaccgtaa tggtcatcgg ctcgcactgc ttatccgtca cggtcacttt gacttgcgga
    10201 atatcagcgg cgttagccat aaaagcagaa gagaacagcg cagccacgct caactgcaat
    10261 gcgttacggc ggaagttaat ggtcatgacg agactatccc tttaaagtat gttgtaacta
    10321 agcaagagtt tgcgtcgtat ttgctacacg gagcgggacg cagtcgcccc tgcgcgtggt
    10381 ggcagaacaa atgccaccag cgccgggatg agataaataa accagacggc gacttcgctg
    10441 acgctcggtg cttcctgata gccaaaaatc ccttccatca gtgtgccaaa cagcgagtga
    10501 gttgagagca ccgcgctcat atcgaaggcg atttcctgaa agtggttcca caatccggct
    10561 tcatgaaatg cgcgaatagc accagctgcc agccctgcgg cgacgaagag aataaacagg
    10621 ctggtccatt taaaaaatgc accaagattg aggcgaatac cgccccagta gagcaggaag
    10681 ccgagcacca cggctgtagc aagaccgagc attgcaccca gcggcggcca gatcccgaca
    10741 tcttgttgaa acgccgccag caggaaaaag accgactcca gcccctccct tgcaacggca
    10801 aaaaagacca tcatcaccag cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg
    10861 actgcctgct ccagttgtac tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc
    10921 caggtaagga tcaccacggc gatcaccgcc acgatacctt caaacagttc ctgttctttt
    10981 tgcggaaatt cgccggtggt ttcgttaatg aagataccca agcccaggca caacgcagcg
    11041 gcaagcaaca cgccaatcca cattacacca atccatcggc ctcgctgggt acgcttaaga
    11101 tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga
    11161 aacggaacaa acatgccacc cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa
    11221 agtaaatcgc agtgatagcg attatcatta cgatgaagaa aaatacaagt ggaatgtagt
    11281 gataatgatg agaagatgta aaaatatttt gagaaaaaac gcagagggca atcactgatt
    11341 gccctctgcc tgcttcctgt tatgacgtga aatttccggt aaaacgcctt cccttaccct
    11401 tcctgcaacc gtgacggcgg agccgaatga tagtgcgcat cggcctcggc aaagcgtttt
    11461 tgcatcgccg ctgacggcgc tttacccagc aaactaaaca ctacaatccc gatactgccg
    11521 aagataaagc ccggaataat ttcgtacaga cccagccagc cgaactgttt ccagacgata
    11581 accgtcagcg caccgatgat catccccgcc agcgcaccgt tgcgcgtcat gcgtgaccac
    11641 atcaccgaga acagcaccac cggaccaaac gctgcgccaa agcctgccca cgcgtagctc
    11701 actaagccca gcacgcggtt ttccgggttt gccgccagcg caatcgccac cagcgccacc
    11761 accagcacca tcactcgccc tacccacacc agctctttct ggctggcctg tttacgcaga
    11821 aacgctttgt ataaatcttc ggtaatcgca ctggaacata ccagcagctg gcaacttaac
    11881 gttgacatta ccgccgccag aatcgccgac agcaaaatcc cggcaatcca cgggttaaac
    11941 agaatttgcg ccagttcaat aaacacgcgt tcagcgttct ggtttaccgc acctgctacc
    12001 gccgggtgct cgttaaagta agcgatacca aagaagccga cagccaccgc ccctgccagg
    12061 cagaggatca tccaggtcat actgatacga cgcgcatgga caatgctgtg gtgagaatct
    12121 gccgccataa aacgcgccag gatgtgcggc tgcccgaagt aacccagccc ccaccccatc
    12181 agcgagataa tggcgacaaa attcaaccct ttgagcatat ccacgttttc gatgctcttt
    12241 tgtttgatta cttccagcga gtcgccaaac ccaccgacgc taatgatcac gataaccggc
    12301 gtcaggatca gggcaaaaat catcaggctg gcctgtacag tgtcagtcca gctcaccgcg
    12361 aggaaaccgc caataaaggt gtaaaggatc gtcgccgcag cccccgccca cagagccgtt
    12421 tcgtagctca tgccaaaggt actttcaaac agacgcgcgc ctgccacaat gcccgaagcg
    12481 caataaatgg tgaagaacag caaaataacc agcgcggaga taatgcgcaa aatgcggctt
    12541 ttatcttcaa agcgcccggt gaaataatcc ggcaatgtta aggcgttatt gttgtattcg
    12601 gtatgcacac gcaaccgccc ggccaccagc ttccagttga tccacgcgcc taatgtcaga
    12661 ccaatggcga tccagctttc ggaaatcccg gaaagaaaaa cagcgcctgg caaccccatt
    12721 aacagccagc cgctcatatc cgacgcaccc gccgataatg ccgtcacgaa tggcccaaga
    12781 ctacgaccgc ccagaatata gtcgtcgaag tttttcgttg atcgccaggc gataaaccca
    12841 atcaatatca tgccaaagat atagacacaa aatgtcacca acatcggtgt gctaatagcc
    12901 atctaaagtc tccaaaaaat tattatcggc aatgtcgaaa cttgccgtta tatctgccac
    12961 cggaacgggg taacagagtt tatgttttac cagggcgacc gtatcctgcc ggaagcgctg
    13021 gt
//
LOCUS       DAZFRG010000135        13020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFRG010000135 DAZFRG010000000
VERSION     DAZFRG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.135"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..797
                     /locus_tag="ACHKUC_003105"
     CDS             60..797
                     /locus_tag="ACHKUC_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6619320.1"
                     /translation="MKSIMKFFACAVLVMSCLTAQVNAASGDSTVSLGFAHIRFPGLK
                     DFVRDAGVYNRDTFRNVVNVNHFNSSAEYENAIARGHDGTAKSPLGMSIRYRYEITDE
                     LGVIASFTWARSMTNAQAFIDVKPSDPSREVKKPAASARTDIRANYWSLLSGPSWRFN
                     ECLSVYAMAGMGVAKVTTDLKINDNLNHGAGSFSESNSTKKTSIAWSVGAQFNFNESV
                     TMDVTYESSGSGDWKTDGLFAGIGLKF"
     gene            1030..1170
                     /locus_tag="ACHKUC_003106"
     CDS             1030..1170
                     /locus_tag="ACHKUC_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6619321.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            1227..1628
                     /locus_tag="ACHKUC_003107"
     CDS             1227..1628
                     /locus_tag="ACHKUC_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619322.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSKSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            1722..2378
                     /locus_tag="ACHKUC_003108"
     CDS             1722..2378
                     /locus_tag="ACHKUC_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619323.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     KKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVIGQIDTTTRGQVSQADKYIAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGNGGGGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            2381..2827
                     /locus_tag="ACHKUC_003109"
     CDS             2381..2827
                     /locus_tag="ACHKUC_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619324.1"
                     /translation="MEYGKYETLARAGYSGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPNSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGKLVSA"
     gene            2837..3088
                     /locus_tag="ACHKUC_003110"
     CDS             2837..3088
                     /locus_tag="ACHKUC_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO6619325.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAMGAAVGGLPGAAIGAVIGGIAGSLF"
     gene            3099..4361
                     /locus_tag="ACHKUC_003111"
     CDS             3099..4361
                     /locus_tag="ACHKUC_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619326.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKTQQLLQKWQTWAAGDPVKQEFVKATAANMPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASASAEKIKGWMK"
     gene            4446..12824
                     /locus_tag="ACHKUC_003112"
     CDS             4446..12824
                     /locus_tag="ACHKUC_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO6619327.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFNNPENAVDH
                     STSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDALVNNPVKTLAGLSSFMTDVYMPPS
                     STQKELGAPPSVGDAIKNIPDGTGKNAVTDTVGKGLKATGKAVSDGAKASDEWLTDQM
                     SPGAVRALNTPMSEGYDDSAVLLTKGVNLIGALVPDLVAGGVVRKVGQVALKRTLTAG
                     LEKKYIAAGVQPERATALAAEAVDKKMTDFFHAGLITHSTASAQGQSAMSAADAVLNA
                     DYAELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLAMRGTAKTVKSGIVRNATEQGTINAAQGGYSRYQENTALRETAG
                     MDVSPGEGVADATIEGAALGAAMGAPFGAVAGYRGKRQAAEETAMREAEVVQQGEAAP
                     QPEPADPVAQHRESMQGMNREQLLEQYADADMAPEGDTSAVHRREAASQLLNELDEQA
                     KRQAVMDELKAKPRPELLEEYRKLSLKEGRTDTEEQQLQAIRDVLRPQQEARPEAQPQ
                     PENADDGDGSIYPTVRFRDPDEVRIEINGSGASRPAERIEKVRPDNRYFTDEKSALGS
                     DVFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPENAAGRQ
                     GEILEGETVRRGLPSPDEQNATALVREGLPAPDITHNVRMPQPDQLPRTVRNSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLASEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDKASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLTKAKA
                     WFAEQEEKSRAKGDEKTAPSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRV
                     FKDGRYVDAEYPVRQEVYDAFKGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARTAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTL
                     KTRKTDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSD
                     LKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEI
                     IHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIRDAWRKVDASYGKESPEVQAGEFLAH
                     MAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTP
                     REDGAGASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVLR
                     HLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHNPDAIYRSATERNAVVML
                     LDAVDKNGDPVVSAVHMKATDKRLEINKVASVYGTENGKKLKSMEMAGLTLYRKEKLS
                     RDNPQYSGLQLPKEERSYRGSVDKILYPEDIRKGAYYSRTSSLTPEETIASRFVRQMQ
                     DKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLAN
                     YKISQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQ
                     YDRLAGIVDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTG
                     KGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKD
                     YWQVFTDDRPDTMRTIAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIK
                     LHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMN
                     LKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFV
                     EDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENAL
                     RLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVR
                     TLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKV
                     PSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGN
                     VVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFG
                     TPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTGR
                     FISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKS
                     LTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSV
                     ERDMKIAVDRFNREYNKKVGVE"
BASE COUNT         3371 a         2976 c         3888 g         2785 t
ORIGIN      
        1 tgtaaaacga aagcgttcct ttcagacatc gcatccttta attcagagaa atacttaaaa
       61 tgaaaagtat catgaaattt tttgcgtgtg cagttctggt gatgagttgc ttaactgcac
      121 aggtaaatgc agcatcaggc gatagtacgg tatctctcgg gtttgcacac atacgcttcc
      181 ccgggctgaa ggattttgtt agagacgcgg gtgtttataa ccgggatacg ttcaggaatg
      241 ttgtaaacgt aaaccatttt aattcatcag ctgaatacga aaatgctatt gcccgggggc
      301 atgacggcac agccaaaagc cccctgggga tgagcatcag gtatcgttac gagataactg
      361 atgaactggg tgttatagcc tcttttacat gggcgcgctc catgactaat gcgcaggcat
      421 ttattgatgt taagccttcc gatccatcac gagaggtaaa aaaaccggca gcttcagcga
      481 gaactgatat cagggctaat tactggagtc tactatccgg tccttcgtgg cggtttaacg
      541 aatgcctcag tgtgtatgca atggctggca tgggggttgc aaaagtcacc actgacttga
      601 aaattaacga caatcttaat catggtgccg gtagtttttc tgaaagtaac agcaccaaaa
      661 aaacgtctat tgcatggtct gtcggcgcgc aatttaactt caatgaaagt gttactatgg
      721 acgtcactta tgaaagctca ggctctggcg actggaaaac tgatggttta tttgcaggca
      781 ttggcctgaa attctgagta atacccgaca acatgtcata acccgctgtg atggcgggtt
      841 ttttgttgcc cgtgcagggc aaaaatcgta gattatgcgc gggtgccttt cggctgatgg
      901 ctggagggag aacctgaagg cctgatgtgg aaaggccccg agtcaaacat tttgtttaac
      961 ccgaggccct aaccatcata ccttaagcaa gtagaaggtt agcgcctctc cactcaggag
     1021 gcaagcgcta tgtcgcaaaa atcgctcatc accgtcacaa tttgtatgac ggtaatcttc
     1081 accatctgga tgttacacgg ctcgttgtgt gagttccggc tgaatttgtg gggagcggag
     1141 tttgcggcgt tcttacagtg taagcagtag gaaaaccgtg acggggacga aagtccccgt
     1201 caactggttg ctgaggttca gccgatatgg cacccgtttc aggtgagaga atggacgaca
     1261 aaattctccg gtatatgcag cgtgttgtga gaaattcccg caaccctgaa tttatgaatg
     1321 aagttaaaga cgcctgcctt aaaaagcagg cgttttgctt tgaagcgccg gatggttttc
     1381 tggtgctgcg ttctgtgctc agtgacgatg gtatccctta tgttctggtg ttactgggcg
     1441 tgtgtacggg gagtaagagt gttgagcgtt atctgccgga ggtgaagaca ttaacccgcc
     1501 tggctggcgg acgctgggcc gaatttcata cggcaagacg aggatttatc cggctgggaa
     1561 aacgtctggg ctttgagcga atgccggatg atgaggatgg cttcatggtg ttcaggatag
     1621 cagtctgact gccactgtat tcatcatcga gcgtaaaccc acattgcaat tcacattctg
     1681 accctgctcc ggcagggttt tttgttatct gaggagccat tatgggcgga agtaaaggtg
     1741 gtggtgatac caaagtaaaa ccaacagcag cgcaaatagc acaggaagag gtggcctgga
     1801 aagggtggca ggattacaaa aatattctcc gtccggcaga agataacttc atgaaaaagg
     1861 tcgatgacct taacagtgag cagcagtacg aaaatatcgc aggcacaaca aatcttgggt
     1921 atcagaaaca gtttggtgaa gcgcgaaggg agctggcggg taatcttgct cagtccggcg
     1981 ttgacccatc cagtggtcgt tttaatgcag taatggatgc aaatcaaagc gaccaggtga
     2041 tcgggcagat tgacacgacc acacgggggc aggtatcgca ggcagataaa tatattgccg
     2101 ggctacagga tgttgcggct ctcggttccg gtcagaaggc agatgcgtta cagagtttta
     2161 actccctggc tgacagcagt ctggcaaaag ccaaatcgga tgcacaggcg gcgtttacga
     2221 aacaacaggg gcgagcttct cttgttggcg ctggtctggg agctgtaggt gcgtacgcga
     2281 tgcataaggc tggcggtaac ggaggtggtg gcggcgctaa aacgccaggc accggcgcta
     2341 atgctattca gcatcaggct cagaactgga gactgtaatt atggagtatg gcaaatacga
     2401 aactctcgca agggctggtt attcaggcgc agcccgccca cagggtgact ggcagacatc
     2461 cgccgcgctg acacgccagc aatacgacga ctggcgaacc agatatttgc cccgcgtggc
     2521 aaggctggct gaccttggtg agaacaacag cctgatgaat gcacagcttg cccgggtagg
     2581 tggccttgcc acttccagtc tccgtacagc gcagatggcg caggataacc agatggcacg
     2641 atatggcgtt aaccgtccgg ataatcccaa cagtaacacg ctggggttac gtaatgcact
     2701 ggcaattgct ggcgcgaaaa atggtatacg ggaagcagag caggatcgcc agatgaacat
     2761 actgactggt gcttctgcac ctgcaagaca gcaactgagt gttggtggaa aactggtgtc
     2821 agcgtaaggg ggcaatatgg gatacggttt actggatatt gcgaatcagt cgcggcgtga
     2881 ggcattacag ggaataagtg atgccgacag acgacgtgaa gaaattgagg ccgcgaacaa
     2941 acagatggcg gcgcaacaga aagcgcagaa caaacagaat atcggcacgg gaattggtac
     3001 gggtgcagct atgggcgctg ctgttggtgg tctccccggt gcggctattg gtgctgtcat
     3061 aggtggtatt gctggttctt tgttttaagg agtggtgaat gagcggattt gcacagggtt
     3121 tacttgccgg attcagcacg gttgaccagg caatgacccg tcgtaaggaa cttggtttgc
     3181 gagaagcaca gcttgctcaa caacagaaaa ataacgagcg cgattttgaa tttgcacagt
     3241 cgcagtttga acataataaa gacgttgatc agcggaactt tgattacaga gccaaagtcg
     3301 acgaccgtaa ttatgcactg caggaaaggg agtttaacgc caaccagaat taccggaatg
     3361 cgtcgctggg gatggaacag caacgactcc agttgcagaa atacaaccag cgacggcttg
     3421 agtataacga tatgctggcg cgcgatcagc ctgtgatggc ggcgctggga aaagcgattg
     3481 atgcgggtga ccatgatgcg gcttctcatc tgttcggtaa attatcggac gccaatccgc
     3541 ttaagatgat gtcaacggta ggctatgctg cgaaagcggg tcaggccgtg aacaacctgc
     3601 agaaaatctt tgatgataag ccggacaggg cgattgcttc gctgaatacc ccggaaaatc
     3661 tcgatgtgct ttccggcgtg tttgccccgg aactacaaca gcgtattggc atgcctgatt
     3721 caaccgggga aaaaacgata aaagaggcca ggattggcag tatcgtaccg gcgcagcagg
     3781 aagggtacgt acttattggc cttgatctca catacagcga tggctccacc gcgcataaac
     3841 ctgtaacaga atacggcagt gcgcaccctg atgatcaaac cgtgctggcg atacccgttg
     3901 ataaggctat cgctcaggtc agggatcgca gcaaatttgc agagatatcg aaaaattatg
     3961 gttattttat gccgaagcag cagggacttt ctctgaaaga gcttcagaag ggggccagca
     4021 acgtagcggc ggacgcgatc aagaatggcg gtaatgcaca ggctgcggtg gatgaatatt
     4081 atgctgcgac tggttcacaa ccgcatcaac agaaaattca gcaacagaaa acccaacagc
     4141 ttttacagaa gtggcagacg tgggcggcgg gcgatccggt taaacaggag tttgtaaaag
     4201 cgactgccgc caatatgcct gctttgctgg aacctggtcg tgaaaaggag gtggaaattt
     4261 attaccagaa tcacctcagg gataaaaagg cagagcatga aaaggcgctg gattatagcg
     4321 cttccgcatc tgccgagaag atcaaaggct ggatgaagta aaaatacgcg gcagtaacgc
     4381 actcaccgtt aatctcgttc acttacgccc gaccatcgtg tcgggctttt tttactggag
     4441 tctgtatggc tttttcagat gagcagcgcc ctgaagcgca attgggtaac cagaaccgca
     4501 gcagtctgaa cattaagcag ccgggagaaa attactggca ggattttttc aacaacccgg
     4561 agaacgctgt tgatcacagc acgtcgttca gcctgggcga tgtattgcca acaatgggta
     4621 aaggtttcgc ccagtccgtc cggggaacag gggaaatggc ccgtggactc ggtgatgcac
     4681 ttgtaaataa tcctgtaaaa acgcttgccg ggttgtcgtc tttcatgacg gatgtttaca
     4741 tgccgccttc ctccacgcaa aaggaactgg gcgcaccacc ttctgttgga gatgcgatta
     4801 aaaacattcc cgatggaaca gggaaaaacg cggtgactga tactgtcggt aaaggtctga
     4861 aggccacagg caaggcagtc agtgatggtg ccaaagcctc ggatgaatgg ctgacagatc
     4921 agatgtcgcc gggtgctgtt cgtgcgctga acacgccaat gagtgaaggc tatgatgatt
     4981 ctgcggtctt gctgacgaag ggtgtaaacc tgattggtgc gcttgtacct gatctggttg
     5041 ctggcggtgt ggttagaaag gtgggacagg tcgcactgaa aagaacgctg accgccgggc
     5101 tggagaaaaa atacatcgcg gcaggagtgc agccggaaag agccacggca ctggcagcag
     5161 aagctgtcga taaaaaaatg acggatttct tccatgcggg cctgatcacc cattccacag
     5221 ccagtgcaca ggggcagagt gcaatgtcgg cagcagatgc tgttcttaat gcggattacg
     5281 ctgagctggc gcagtcaccg aaatttcagc agacgttttt gtcaattgat gcagatccac
     5341 agcacgcaca gcttactgat cgccagaaaa tggacctggc aaaagagcgt gttgccgatg
     5401 aggtgcgcgc gcagctggca accgatcctg aattgctggc tgtgaatgcc atggcggcaa
     5461 aactgggcga tgctcagttg tttaatctgg cgatgcgggg cacggcgaag accgttaaaa
     5521 gcggcattgt cagaaatgcc acggaacagg ggacgattaa tgcggcgcag ggtggctatt
     5581 cacgctatca ggaaaacacg gcattgcgtg agaccgccgg aatggatgtg tcaccggggg
     5641 agggcgtggc tgacgcaacg atcgaaggtg cagcccttgg tgctgcgatg ggcgcaccgt
     5701 tcggtgcggt tgccggatat cgtggaaaac gtcaggccgc agaagaaacc gccatgcgtg
     5761 aggctgaagt cgtacagcag ggtgaagcag ccccgcaacc cgaaccggct gatccggtgg
     5821 cacagcatcg cgaatccatg cagggcatga atcgcgagca acttctggaa cagtatgctg
     5881 atgcggatat ggcacctgag ggagacacgt ctgccgttca tcgccgggaa gccgccagcc
     5941 agctgttaaa tgaactggat gaacaggcga agcgacaggc ggtgatggat gagctgaagg
     6001 cgaagccgcg ccctgaactg cttgaggaat accgaaaact cagcctgaag gaagggcgta
     6061 ctgatactga agagcagcag ttacaggcta tccgtgatgt gcttcgccct cagcaggagg
     6121 ccagaccgga ggcacagcca cagccggaaa atgcggatga tggcgacggg agtatttacc
     6181 cgacggtgcg gttccgcgat ccggatgaag ttcgtattga aattaacggg agtggtgcgt
     6241 ccagaccagc agaacgcatt gaaaaggtgc gtccggacaa ccgttatttc acggatgaaa
     6301 aaagtgctct ggggagcgac gttttccgca atgccagcgc caccggcctg aaaccgtccg
     6361 tagtgaagaa aggcgagaat cagtatgccg ttgaaatgga taatcctgcg ttctctgaag
     6421 atgtggcaac ggagaccatt aacactctgg ctgacggaga gcgtattact gatgctgatc
     6481 cgatggaaca gcccgcgttc atgcgtgacc cgcgattccg tggttttacg ggggatgata
     6541 cagaagtgca ggcccgtctt gcccgtggca acgcgccgac ggcggaagaa ctggtgcgtt
     6601 cccagatggc tgaaggtgat gccggtccga cagcacagga gttaactgag cgtccacgcc
     6661 ttcccgctcc cggcgatatt catcccggac agggatatcc gttaccggga gaagtagcgc
     6721 gtacgccgga tgaaaatcag gccggacgtg gtggtcgttt taccacaacc ggtgaggtga
     6781 agggccagag tttccagaaa ggacgtgcgc aggcaccgga aaatgccgct ggtcgccagg
     6841 gggaaatact ggagggcgaa acagttcgtc gtggtctgcc gtcaccggat gagcagaacg
     6901 ccacagcgct ggtacgtgaa ggtctaccgg ctccggacat tacacacaat gtccgtatgc
     6961 ctcagcctga tcagcttccc cgaactgttc gtaactcatt gcctgagctc gcacagcagg
     7021 cagaagtacg tcgacaggcc ggaggaaatc gtgacatccc gcagcctgag acaatcgcac
     7081 ctgaatctga aacaactgtc tctactgaca gggaagctac tgtgcgcgga ggtgaagtca
     7141 ggggcaaaaa aattgaagac tttggcgagg aaattaaagg ggcagccaaa caccgttatg
     7201 cacagcttgc tgaaacactg ggtaaaacgc tggaagacag ggattatgcc acgcagccgc
     7261 tgagcaaact gttcccgaaa ccggactacg caaaactggc gagcgaaggg gctgatgctg
     7321 acaccctggc aatgatagcg ctgtatcgta gcgatattcc ggcgaagacg aaacacaata
     7381 cggctggctg gggggagagc gtaaaaaaag tacgacacag tgtatcggaa atgctgaacg
     7441 gaacggtcag cgcgaaacgc ctcgcagaat ggatggaagg cagaatgccc tcccgttacg
     7501 cggatacctg gcaactgtta cgcactctgc caccctcaca gatggacaag gcttctgctt
     7561 atcgggtggt atccggtgtg tatcaggcgg ccggagggaa gcgttacgat ccgccacaga
     7621 aactttattc actgcgcaat aaggacaata aggggagtaa cctctttttc tcggaaagca
     7681 gggatgaatt actgacaaag gcgaaagcct ggtttgcaga gcaggaggaa aagtcacggg
     7741 cgaaaggtga tgaaaaaaca gcaccgtcac cagatgacaa aatcagcttt gacgtttacc
     7801 ggaatacccg tagtggcgat atttttatcg cttacggcaa aaacaaaatg cgggtgagag
     7861 gtggttttaa gtcagccagt gatgcgcgta agtacattga ttcacatcgt gatgagcttg
     7921 ttcgtcatgt gaaggagatg cgggagattt cgcgtgagga gcagcgtaac gccactaacc
     7981 gcgaccgcac cggaccagaa cgccgcaaag gggatgtttc gccggagcag ttcagtgatg
     8041 cgtttggttt ccgtggtgtg cagtttggta attatgtgga aggtccgcgt cgtcaggctg
     8101 atttgaaccg ggcttatgac tcgctgcatg acctggctga cgtgctgaac gttccgacaa
     8161 aagcgctttc cctgaatggt cgtcttgggc ttgcttttgg tgcccgtggt aagggtaagg
     8221 cggcggcaca ctatgagccg ggtgaggtgg caatcaacct gacaaaaggt aacggaccgg
     8281 gggcgctggc ccacgaatgg ttccattcac tggataatta ttttggtcgt tatgatgttt
     8341 ccactgacgg gaagatcacg tcaggtggcg actatatgac ggaagcacaa cgtgccgggc
     8401 gcgtatttaa agacggcaga tatgttgatg cggaatatcc ggtacgtcag gaggtttacg
     8461 acgcgtttaa aggcgtgatg aaagcgatca acagcagtga catgccgcgt cgttcagcgc
     8521 ttctcgatga ggtgcgctca aaaccgtact ggtcaacgga tgttgaaatg gcggcgcgtg
     8581 cctttgagcg ttatgttcag gataaagcgc gtacggctgg cgtggagaat gattatctgg
     8641 tcaatatccg taaggcacct gagcacaaca cagataacac ctgggcttat ccgacgaatg
     8701 cggaactgga tggcggtatt cgtgatgcat tcgatcacct gttcagtacg ctgaaaaccc
     8761 gtaagacgga caagggcgtt gcgttttatt cccgtaaggg cgtgacccgc acacctgaag
     8821 gcaatctcat ttcggatgtt aaccgcagtg cggaagccaa aggcagcccg gtcccgcagg
     8881 ttgaagcggt tgcccgtggc gtgatgagtg gcattaagga cagtgacctg aaggtccgcg
     8941 tggtgaagtc acagaaagag gctgaagcgc tggcgggtga atcgttcgac ggttacggca
     9001 gggtgcatgc gttctatcgt ccggataaac gtgaaattgt cctggtggcg gataacatcc
     9061 ctgacgggcg gaccgttcgc gagaagttac gtcacgagat tattcaccat gcaatggagc
     9121 atgtagtcac gccagcggaa tatcagacga ttattaagac cgtactgaaa acccgtgaca
     9181 gtgataacgc caccatccgt gacgcctggc gtaaggttga tgcgtcttat ggtaaggaat
     9241 caccggaagt gcaggcaggt gaatttctgg cacatatggc ggagaaacag ccgaataaat
     9301 ttgtggcggc gtgggagcgt gttgttgccc tggtcaaagg ggtactgcgt cgcacggggt
     9361 tactgaagcc gacagagctg aacgatatca gacttgttcg cgaaactatt cgcacgttag
     9421 gccagcgtgt gcgggaaggt tacacgccgc gtgaggatgg cgctggcgca tcgtctcagt
     9481 actcccgtag cggtaagcct gatccgttca aagtgccgga aggtgagggc gagcgttatc
     9541 gtgatgacct tgccagaatg atgaagtcgt tacgttctgg tgcaatgact gcaaacatcg
     9601 ggcgtacgcc gccggtattg cgccaccttg gcgcaccaga tttgccgctg gttatctccc
     9661 gcgacactgt gcggaaggct accaatggcg tgaaacatgt ggtgccaatg gatgttatcg
     9721 agagacttcc ggaactgatg cacaatccgg atgcaattta ccgttccgcg acagaaagaa
     9781 atgcggttgt gatgctgctt gatgctgtgg ataaaaatgg tgatccggtc gtctcggcag
     9841 tgcatatgaa ggcgacagat aaacgtctgg aaattaacaa ggtggcatct gtttacggta
     9901 cggaaaacgg gaaaaagctg aaaagtatgg aaatggctgg attaacgctt taccggaagg
     9961 agaaattaag ccgcgataac cctcagtaca gtgggctcca attgcccaaa gaggagcgtt
    10021 cttatcgcgg ctctgtagat aaaatactct atcctgaaga tattcgcaag ggggcgtatt
    10081 actcccgcac cagcagtctg acaccggaag agaccattgc atcgcgtttt gttcgccaga
    10141 tgcaggataa attccaggtg ctgaaggcgg tgcaggagaa tatccgtaaa actggcggaa
    10201 aaatagatga cagcaataac gcctatatgg ctgaagaact cttccacggg aaggcggaaa
    10261 acgacctgaa cgtaatgaag gagcgctacg ttcagccgct ggccaaattg ctggcgaact
    10321 acaaaatctc tcaggccgat ctggatgagt acctctacgc ccgtcacgcg ccggaacgta
    10381 acgcacatat tgcgaaaatc aaccctaaaa tgccggacgg cggttcgggg atgaccaacg
    10441 cggaggcggc ggaaatcatg cagcgtgtgc gtaacagcgg caaacaggca cagtatgacc
    10501 gtctggcagg gattgttgac gatatgctgg cccgtcgccg tgagcttatc cgtgaggccg
    10561 gacttgaaga gaacggtgtg gtggatgcct ggcagaacgc ctaccgttac tacgttcccc
    10621 tgaaaggtca ggatgttgac ggtgtggtgt cacttccccg tacaggtaag ggcttcacca
    10681 tcggcggacg tgaaagcagg caggccatgg ggcgtgcatc ccgtgcacag tctccgtcca
    10741 ctcaggcgat acaggacctg agcgaatcgc tgatccgcca tcgcaaaaac gaagtgggta
    10801 acgccttcct gaaactggtg caggataatc ccgacaagga ttactggcag gtattcaccg
    10861 atgacagacc ggataccatg cggacgattg cagagcgcaa ggaccaggaa actggtgaaa
    10921 cccgtcgcga agttgtcgaa cgccctgtac cgatggcaat gatggcagac cgctacttca
    10981 ccaccaaaaa gaacggcaaa acgtactaca tcaaactcca tgacccgcgc ctgatgcgtg
    11041 cgatgaagaa tatgggaccg gaaaccagta acgccgtaat ccgtacgctg gggaaagtta
    11101 accgcttcct ggcaacggtg aacacgtcgt ataacccgga attcctggtc agtaacttca
    11161 tccgtgacgt gcagacggcg gtgatgaacc tgaaggctga gcagggaagg agtgacggta
    11221 aactgaaagg gctggataac ttatccgccc tggctgtagt caaagacagc cgttctgcca
    11281 tgtcagccgt atacgccagt ctgcgtggta aaaccctcac gggcaaaggt gcacagtggc
    11341 agaaggtgtg gaaagagttt gttgaggacg gaggtaaaac cggttggttt aacatgggtg
    11401 accttgaagg ccagcagaag gaaatggatc gccttgtttc gctggcgaag ggaggatgga
    11461 aaggccagag tatcggtgca tggaattcgt tccttaacct tgtcgaggat gccaacggcg
    11521 cggttgaaaa cgcactgcgt ctgtcagctt ataaacacgc ccgtgatgcc ggtttgtcac
    11581 gccagcaggc ggcgtctctt gccaaaaaca tgacggtgaa ctttaaccgt cgtggtgagc
    11641 agggggcgct gatgaactcg ctgtacatgt tcgccaacgc cagtattcag ggaacggcaa
    11701 atctggtgag aacgctcgga catcttaatg gtgagggacc tttactggag cgccttcgct
    11761 ggaagaatct gaatgttccg cagaaaatcg cgcttgccgc tgtgggagca ggttatctgc
    11821 ttggctcgct taaccgcagt gtggcgggtg aggatgatga cggggttaac tggtatgaca
    11881 aggtgccgtc tcatgtgaaa gagcgtaacc tcgtcattat gaaatcggtg ttcgggggca
    11941 aggccggaga gtactggagt attcctctgc cttacgggta caacgttttc ttcctgctcg
    12001 ggcataccgc tgaaggtgtg gcggcgggtg acctgacggc gtcccgtgct gccggtaatg
    12061 ttgttggtgg tgtgcttggt gcattcagcc cgattggcag tgagacgtcg gaaacactgt
    12121 ccggggcatt gctgaaaaat gcagcgccga ccattctgcg tccgtttgcg aaccttgcca
    12181 tgaatgaaaa cttcatgggg gcgcagattt accaggagaa catgccgttt ggtacaccaa
    12241 aacctgacag ccagctggga agacgttcaa cgccagaagc gtacaaggcg tttgcatcct
    12301 ggctgaatgc gttctcaggt ggcagccagt accgtcccgg cgcggtggat gtcacgccgg
    12361 aatcactgaa atactggatt gactatatct ctggtggtac agggcgcttc atttccaaaa
    12421 ccacggatgc ggcggtgaaa tcgctgaatg gtattgatat accggagcag caggtgcctt
    12481 tcctggggaa aatttcaggt gaggtgatgc cgtatgctga ccagcagaag atgtacgacc
    12541 ggatgacaga ggttgcgcag taccacgcag agctgaagag tctgaccggt gcagaaagaa
    12601 cggcgttcat tgacgagaac aacggaaaat tgtcgatgaa cgggcttatg caggataccc
    12661 ggaagagact gaaggatttg cgtaaacagc gtgatgccat ttacgccgac agtactctca
    12721 gtctggcgca acagtcggcg atggtgaaat cggtagagcg ggatatgaaa attgccgtgg
    12781 atcggtttaa ccgcgagtac aacaaaaaag tgggagtgga gtaaagaaca tgctccgtac
    12841 ggaagtgcgg ggctgatata aagtcacgca gggaaatatt gtagatgaac tgaacagtta
    12901 aattcggata atgttgtgat attgatcaga aattacagtg taagcagatg tgaaacagaa
    12961 tgcctgatga gagtaagttt tttctgatgc ctgttccggt ggtgtagtcg tggttctgag
//
LOCUS       DAZFRG010000136        12842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFRG010000136 DAZFRG010000000
VERSION     DAZFRG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.136"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..1418
                     /locus_tag="ACHKUC_003113"
     CDS             246..1418
                     /locus_tag="ACHKUC_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619328.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAIKRHKQQLGAIEEYTQWL
                     KKEPRASYFFLFRLYTRIHNTFFPKKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSAVVPPTNENTSPPEDTPDNTPARGNTGQAEKTRNSGLTPIPEKRSGM
                     PPEHLRFATGFPPQPKIAGPKGKPMRTVHPDKIYREIIWFCSGYLRKSGPEATRTIIN
                     SIFCEWASIFNDYSSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRR
                     DERADGSVPLKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(1949..2206)
                     /locus_tag="ACHKUC_003114"
     CDS             complement(1949..2206)
                     /locus_tag="ACHKUC_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619329.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(2260..3027)
                     /locus_tag="ACHKUC_003115"
     CDS             complement(2260..3027)
                     /locus_tag="ACHKUC_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6619330.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(3024..4082)
                     /locus_tag="ACHKUC_003116"
     CDS             complement(3024..4082)
                     /locus_tag="ACHKUC_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6619331.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(4101..5090)
                     /locus_tag="ACHKUC_003117"
     CDS             complement(4101..5090)
                     /locus_tag="ACHKUC_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6619332.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(5101..7266)
                     /locus_tag="ACHKUC_003118"
     CDS             complement(5101..7266)
                     /locus_tag="ACHKUC_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6619333.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVDDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSIGHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            7695..8129
                     /locus_tag="ACHKUC_003119"
     CDS             7695..8129
                     /locus_tag="ACHKUC_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619334.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIHLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            8347..10731
                     /locus_tag="ACHKUC_003120"
     CDS             8347..10731
                     /locus_tag="ACHKUC_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO6619335.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMETEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WECSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELLTGVYTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            10728..11633
                     /locus_tag="ACHKUC_003121"
     CDS             10728..11633
                     /locus_tag="ACHKUC_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO6619336.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            11630..12700
                     /locus_tag="ACHKUC_003122"
     CDS             11630..12700
                     /locus_tag="ACHKUC_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO6619337.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
BASE COUNT         3188 a         3375 c         3380 g         2899 t
ORIGIN      
        1 ccggagaata ccagtcaggc ttaaaaagac aataaataca gtgagttaaa tacacagaga
       61 aaacgaaaac gtagtatcag agggtacatc ttacaacaga gactgaattt ccggtttttc
      121 ataaccggcc attccggaaa gtgcaggcgg cccggagctc ttctgacgcc agtacctgcc
      181 agattgttct gtacattctg tacatcatgt acggaggttt ttgtttgctt tcttaaaatc
      241 tgcaaatgtg ggaagagcag gtgaggtgta ttgtgggaaa ggaactggat gatatctgca
      301 ccagctgtcc gtatatcgat gctataaaac ggcacaaaca acagctcggt gccatagagg
      361 aatatacaca gtggttaaaa aaagaaccac gggcatctta tttttttctg ttccggctgt
      421 atacccgcat tcacaataca tttttcccca aaaagcaaca actcccgttc actcccggtg
      481 gcactcactg cccggaacct gatgtcaccc ttcgggactt aacgctttcc ccgggctacc
      541 acagtgatta tgcccctcaa cccattccgg agatggacag ttctgctgtt gttcccccaa
      601 ctaatgaaaa cacctcgcct ccggaagata ctcccgacaa cactccggcc agaggcaata
      661 ccggacaagc ggaaaagacc agaaactcag gactcacacc cattcctgaa aaacgttcag
      721 gaatgccccc ggaacatctg cggtttgcca cagggtttcc accgcaaccg aagattgccg
      781 gtcccaaagg aaagccgatg cgcaccgtgc atccggataa aatctaccgt gaaattatat
      841 ggttctgctc cggttacctg cggaaatccg gaccagaggc tacgaggacg atcatcaact
      901 caatattctg tgaatgggcc agcatcttca atgattactc ctcccccttt tcgtgggtgg
      961 acagcaggga cagtgaacag tgtgactggt tatggaacgc catgcaggtc cggtgtgtgg
     1021 gaaccccgct gaatcccctt accccggagc agaaatactg gttcgcctgc gccacgtttg
     1081 ataactggga aggctggaat gagcaacagg tacagttttt actggaaagt aatcccagac
     1141 ggaacagagc gaagtttacg caggcctcct tccaggcccc caggattcag cataaagcga
     1201 ttcttcttga tgagctgaaa agtgcccggg agcaacaaaa aaggcgtgat gaacgtgctg
     1261 atggttccgt cccgctgaaa ctgtccggaa aaatccacaa acagcttgaa agtattgccc
     1321 gaagtcgtgg tgtcctccca aaaaaactgc tgaatgaaat gattgagcag gcgtaccagg
     1381 actttgtggc aaatgagcaa cacaagacac tgagttaagg accgcagggc ctccggttac
     1441 cgattctgca gcatacacaa cgaatgtgtc cggctcttac ctgtgggggc cgcactaaag
     1501 ttacgcattg agtatgagtg ggtaatcccg gatgagaata tcccggtttt tacactattc
     1561 actgaaggtg cgacatatca ttcagtctct ctgaattctt catttcagtg gccccggcta
     1621 cagtcgttta atctcctcca cgaaccgggg ccgcaacttg ttgtgtcaat accaaattgt
     1681 tggtcattac tgtcttgtgt cactggcgct gacaagtgaa ctgaaatccg caccttaata
     1741 ttctgactgg ctgcgtacat tcatggcatg aagcggccgg agcggctgaa ctgaaggtcc
     1801 gttgtgagcg aacagcggac attcactaca catggttcaa acatgaaccg gagctttatt
     1861 gtctagctgt cagtgtactg cgatttaccc gctcggctgt ccagaaaatc ggtctgaaat
     1921 ccagtcatta aagggcctta gcctgacatt aagcaacttg gtaggcggat agataataga
     1981 aataggaatg cgcaggtctg tcatgttgcc tagaacggtg acaagctgtc cggtgcgaaa
     2041 tcattccggc agcgacaggc tcatgtgtca gcccgacgcc cagaccgtca acgagcatgt
     2101 ggatctgcga ggcgttatcg ttcgcagcga tgttaaaacg aacaatctgc tcatcttcta
     2161 gaagcacaag tgctttaatg acattaaggc ggtctatatg ttgcattaca gacttcctat
     2221 tgatctttac ctgaggctga agcaaccacg ttaatccgac tatttttcga ttgcaccatc
     2281 cactatcacc atcgatcttc cctgagcaca acgttcaact ctcccgcgta cgccgtatac
     2341 ctgcgcaagg ctctctgcat ggatcaccgt ttgcgggtcg ccgctgtcga taagtttccc
     2401 ctctttcagc atgatgacct gggcggcatg ccgcagtgcg atattgatat cgtgtaagac
     2461 gaccagcgta accatattgc gtcttcgcgt ttcgcgcgat acaacatcca tcacatggaa
     2521 ctgataattg agatccagcg cgctcagtgg ttcatccagc aataataatg cggggcggcg
     2581 aataagcgac tgtgcaagcc ccaccagctg cttctgaccg ccggacaggc tatcgaggta
     2641 gttcattgcc agatgtgcga tgcctagctc ttcgagcagc gctatagcct gcgcctgatt
     2701 ttgcccggcg ccggaggcgc gctgggcgac aaccaccgac tccagcacct gtaaatgcac
     2761 gccctgcggc agggactgcg gcaggaacac aactttttca gcccgacgag caaacggcag
     2821 cgataagaga ttctcttcat tcagccaggc ttcgccactg gcgcgattta gcccggccag
     2881 ggcgcgcagt agcgttgatt taccgcagcc attaggcccc agcagtacag tgacttcgcc
     2941 gcgcggcagc gtagaaatcg acagatgctc aataatccgc cgtttgccgt aaccggcgca
     3001 cagcgcatta atcgtaagcc cgctcatgac atactccctc gatgacgcat tacaatgctc
     3061 agaaagaacg gaacgcctac cagcgaggta acaattccta ccggtaaaat gacaccggga
     3121 atgatgttct tcgacgctac cgaggccagc gatagcacca gcccaccgat aaggatgctt
     3181 ccgggcaaat aaaagcgatg atcttctccc agcaacaggc gggaaatatg cggtgcaacc
     3241 aggccaataa agccaattgg ccccacaaac gcaaccgaaa gggccgccag caggctgatg
     3301 cgcagcaggg aacccaggcg caaacggcga acatcaatgc caaaactcat cgcccgctct
     3361 tcgccgaggc gtagcgcggt aagctgccag gcacgacgca gcgaccaggg taaaacaatc
     3421 gccatcgccg ccgccagcac cgccagtttc tcccatgatg cgcgagccag gctgcccatc
     3481 gtccagaaca ccaggccctg caaagtatct tcatcggcga caaactgcat aatggacacc
     3541 agcgcattga aggtaaacac cagcgcaatc ccaaacagca caacgcccga cgtcggcacg
     3601 cgcgtccagc gggtcagact atcgagcagc agggcggaca ggagcgcaaa aagaaaagcg
     3661 tttgccggta taaacccgct ttcgggaacg ccgggaatac cgatgcccag aacaatcgcc
     3721 agcgctgcgc caaaggccgc cgccgacgaa acgccaagcg taaacgggct ggcgagggag
     3781 ttattcagaa tagtttgcat ctctgccccg gccaaaccca gcgccattcc taccaggagc
     3841 gccatcagcg catagggcaa acggatgtcc caaacgataa cccgcatgcc gttagtcgcc
     3901 ccggacggat gcatcaaagt ctttatcagc gaatgtaaag ggagccctga ggggccgagg
     3961 gtgaaatcga gcagcagcga agcaatgata gcgataaaaa taaccgccag tattaccaga
     4021 cgctggcgaa aaagctggcg atagcggttc tcgaccgaca gcgtactttc caatacagtc
     4081 atggtttttc acagcctccg ttagcgcggc ggattgagca catcgctcat ctgcgcatcg
     4141 cttaacgtca ggccaaagaa ctgccgataa aactcacgcg tctctttgcg tatatccaca
     4201 tcagcaaatc gcgcaggata aagcgttttc gccagccaca aaaattgcag cgcctcttcg
     4261 ctggtttcac ggcaccacca gaacattcct ttcggattaa cgtaaacgcg atgatgaatg
     4321 accgcgtcca cgcttgccca ctgcggagaa ctcagaatgg catccgcgtc gcgcttgttc
     4381 atcgcgatga taaccgccgg attggcagtg acaattttct ccagcgcgac ttcacccgaa
     4441 cggttttgtt gatgctcgcc aaaccaattt tcagcgatat ttttgccgcc ggccaggtca
     4501 atccagtcct ggtttagcga gggtctaccg gtggtggtca gcggattgcc catactgtga
     4561 tagagcgaca cgcgctgtga cgcaggaaga tctttcaggc gcccggtgac cagggccacg
     4621 ttgcgatcga agtagcgttg ataggcgagc gctttttgct gcgcatcggg ccccagcacc
     4681 gctgccgttt gctgtacgcg ctgcgttaac gcccgcagtg aattcgcctc aaacgccagc
     4741 acctgcaccc cagcctgttt cagcaacgcc tgttgaggca ctaccatatt ctgcggtaca
     4801 aaaagcatat cgacccccag cgcgataatc tgttcgggat tgatttcatg cccgctgttt
     4861 acgctaactg ctgcgacttt ctctatgcgc ggcaggctct ggcgaaacag ggggatcctt
     4921 tttgcaatca tcgaggttcc gacaatgtta tcgccgtacc ccagcatggc aataatggta
     4981 ttttgcgccg gccacggtga cgcgatacgc acctccttgt gcgtattttc tgcgtgcagg
     5041 ctggccgcca gcagcagcgc cagcagcgga aataaacggg agagtttcat catttatcca
     5101 tcagaaatcg aaggtgacgg atgcacgcac ttcgcggccg ccgccgataa acgcacttgg
     5161 ggaaccgtta tcgccatcgg tacccgatgg gaagatagaa gcccagtaat gtttatcaaa
     5221 cacgttgttt accaccacgc ggaaagtggt tgggacgttg ctcactttcg tggtgtaacg
     5281 cgttcccaaa tcccatgtgg tatagctgct ggcgtaagag gtgttggtat cgttcgccgc
     5341 gcgtttgccc gtataatgga cgttagcgct gtaaacccat tccggtatgg acggcaaact
     5401 gtattccgcc aacagattgg cctgcacttt cggcacgccg acaacctgtt tattgctggt
     5461 tgaggcattc gccgtatctt tcagtttcgg gtcgaggaag gtcacgccgc tgtaaatatt
     5521 cagcccctgc cacacattcc cggcagcggt taactcaagg ccgttgttaa cctggttacc
     5581 ctgctcttta tacacgttat ccgtatcaag ataggcaaac ggacgttcca gtcggaacag
     5641 cgcggcgcct agattcatct cgccgatgtc cgattttagc cccacttcat actgcttgct
     5701 gcgatacggg tttagcgttt gaccggcatt ttttacgctc tcatccgtcg gtgcggtacc
     5761 gccctgctcc agcgaatcgg catagctaac gtaggccatt gtgttagggg taattttata
     5821 catcagcgcg gcgttcggac ttaaaccatt ttcatcaacc tggttcgttt gattaccgtg
     5881 cttatcgtag tttttagtct gcagccagct ctggctgaga tagaacattg ccgaccactg
     5941 cggcgtaaat gtcaccgtat cgccgagcgt aacgctctgc tgagtattta cgctggagcg
     6001 gtatcgcggc ccgccggtgc ggattttgcc atcgccctgc tcatctatcg catccgggtg
     6061 atacatattt gttgtacccc agctatagct ggaacctgtt cctttggcac tataaagcga
     6121 ccagacatat cccgtggtag aaagtgacag atcgtggccg atagagccgg tatcaatatg
     6181 accattcagc ccggcggtgt tgctcaggac gcgaaaacgt ccggcagcgg tggaatcctt
     6241 catcgaacga gagatatcgc cctgattgtt gagtatttta ctggaaacac tacgcatcgc
     6301 gcggtcagcc tgctgccagc ccacgccagc gttcatggac cagtcgtcgt taaagtagtg
     6361 gatcaggcga gtgctgatgg tatcggtagt gaggtcgttg cccgcagtgc tgagcgccag
     6421 atttttgtcc ttcggattcg gcgcagacgg cagtttgacg ttcggcccat agttaaagct
     6481 accgacatag cctttctgga taaattcgta atggctggcg tcgagctgta gctgagtgcc
     6541 cggctgaata ttccagtcga gcgcaacgga aacgagtttg cgacgcagcg tgctgtcatc
     6601 cacattgcct tccccttcct gatcaagcag gttcacgcga tagccaaacc gtttgttttc
     6661 atcaaaatgg ccacccagat cggcatggcc ggtaaacgca ctgcggcttt gatagcccag
     6721 cgtcacttta cgcagcgtct cttcggttgg gcgcttcgcc acgaaattaa actgccctgc
     6781 tgggctcgcc gggccgtaca gcgcgccggt caaactgtta agcacatcca tgcgctcaag
     6841 catttccacc ggaaacgcgg ttgttgaaac gatattcagc ccgtccagac ggctgttggc
     6901 caccacgctg ccctgcatcc cccgactttg cggacgcccg acatccattc caccgcgcgc
     6961 ctgcatttgc gtactgggag agtatttcag catctcgctg acgctttgcg cctgctgatt
     7021 ttcaatcatc tctttagtaa cagtggtgct ggaatatggt gtttcgatcc aggatttcgt
     7081 tcccagcggg ccgacatcta tatcgttcgt tttgaaaccg gcaccggcgg cagaatcggt
     7141 ggtgtcggcg gcggtgttgc ccgtgacaat catttcatct tcagaagtag acgaggcatg
     7201 agcatgaagc ggtatgaaag acgctaatgc tggcaaaagc gcagcgaaag ttatttgagt
     7261 tttcatgaat cactgatcgt tatgtaaata tttatattac gatcggtgca taggcaaaat
     7321 tatgatccag atcacacgtg aatgtaagtt atttacaaat tacgctaacc ttctgtctcc
     7381 gctctttgca gcataaaagt atggcggcgc gttgttgaaa gcaggagcgg ccgggttgtc
     7441 agaaagctgg tttggacgca agtaatatat aacaactctg attaccgcat actgtcagga
     7501 cagtatctac ttgagcgtcc gcctttggca caaagcggac tgtcagatta gattcagacc
     7561 tgtactcatt agctgataca aatcagccac atatacctgc attcaaaata atttcagaaa
     7621 gatattatct ccgtgaatcc cccgccacct ttccggtgca cggggttttg tcttttttca
     7681 ctgggaatac atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct
     7741 ccctgcctta cccgatttcc cgtcacatcc tggaacatct gcgcaaactc acccgtcacg
     7801 aacccgtgcc tggcattatg ggtaaaagcg gggccggtaa atcctcactc tgtaatgcac
     7861 tgtttcaggg ggaggtcacc ccggtcagtg cactcatgac agtgcttccc ggacatgccg
     7921 ccatccatct gatgacacgc ctgcaggacg aactgcgcac ggagtctgtc cgcactcaga
     7981 cccgtgaaca gtttaccggt gcggtggacc ggatatttga cacggcggag agtgtctgca
     8041 ttgcctctgt tgcacgcacg gttctgcgcg ccgtccgtga ctcggtggtc tctgttgccc
     8101 gcgcggtatg gaactggatt ttcttctgaa cccggtggtg atgtaccagg ctgttgttac
     8161 agatttttaa ccccatgaaa acactatgca aacgcagcca tacccggggt gtccccttgc
     8221 gttgtgttcc tcaagttaat agaatacatc gatcgttact attatattga tcgttataaa
     8281 cgatcgtttg tagtaaatga cctcctccgt ggttgtcatg aaaatgcgtc tcaggaaggt
     8341 taaataatgc acgaaaagaa catcgccctg ctttgtgatg aagccgaccg acttttgcaa
     8401 ctgaacatta atctgctccg gcaaatggtt gaggagccag atgtgttatc tgacagtaag
     8461 aacgaaaaca gactgctttt tgataaacag aaagcactga aaagaattga ggagctggag
     8521 ggcgaacaaa tcaaaaccgc ccgcagggag atggtgctgg ctgttgtcgg cacgatgaaa
     8581 gcaggcaaat caaccaccat caacgccatt gtggggcagg aaattctgcc taaccgtaac
     8641 cgccccatga cctctgtacc gacgctcatc cgccacgttc ccggaaaaac tgagccggtt
     8701 ctccatctgg aacatattca gcctgtccgc aatttattaa tcacactgca ggaaaaactc
     8761 gccaccccgg caggacagca ggtcgcacag accctgcagc aaaccgggga tacccgcgaa
     8821 ctgctggata ttctgacgga tgatggctgg ctcaaaaatg aataccacgg ggaggaggaa
     8881 atctttaccg gactggcatc gttaaacgat ctggttcgtc ttgctgcggc aatggagact
     8941 gaatttcctt ttgatgaata cgcagaagtg cagaaactgc cggtgatcga cgtggaattc
     9001 agccatctgg tggggatgga tgcatgccag ggaacactca cactgctgga tacccccggc
     9061 cctaatgagg ccggacaacc gcagatggaa gtgatgatgc gggatcaact gcagaaagcc
     9121 tctgcggttc tggctgtgat ggattacacc cagatgaact caaaagcgga tgaagacgtc
     9181 cgtaaagagc ttaatgccat tgctgacgta tcagccggcc gcctgtttgt actggtcaat
     9241 aaatttgatg agaaagaccg caatggcgat ggggcagatg ccgtacgcca gaaagttccg
     9301 gcaatgctga acagcgatgt gctgcccgcc tcccgcgttt atcccggatc ctcacgccag
     9361 gcatacctgg ctaaccgtgc gcttcatgag ttacggaaaa acggaaccct tcctgttgat
     9421 gaagcctggg tcgatgattt tgtcagggaa gccttcggtc gcatgaaaaa agattacgtc
     9481 tgtaaagaca gcgaactggc aactgaaggg gcgacagacc tgtgggaatg ctcacttatc
     9541 gatcaactga taacggaagt catactgagt tcacactcca gagctgcggc actggcggtt
     9601 gattctgccg ccgcaaaact gatgcagaat gcagaaaata tcagtgaata cctgtcgtta
     9661 cgccatcagg ggctgatgca aagtattcag tcactgcagg cgcatatcac cagcctgctt
     9721 gaggatatcc gggaaatagc agactgtcag gagcaggtga ccgctgatgt cagaatggcc
     9781 atggaggaga tcgatgccag aacccgggaa ttactgacgg gggtctacac ctcactggaa
     9841 gaggagctga atgactattt cagaagcggt aaacgcaaag aacagcaaat gctggaggaa
     9901 gaaaattcag cgcagccccg ggagagaaac gcattcgctt tctttcacga tattttcgga
     9961 accggaaatc agcatgacag gatgcgggac tttgatcccg acagccccga aataaaattc
    10021 agtgatcgca gggaagccct tgaactgatg acgcaaatcg aatcgaccgt gaccagcctg
    10081 caccgtgagg ctgaagcaca gttccggcct gagctggaga aaatcgtcag cgggattgaa
    10141 acaggttttc gtggcacggc cctgtacgcc acagaaaaca ttgccggtcg catcaatacc
    10201 cgcctggagg atgagggctt taccgtaaaa atcagttttc cggcagtcag tcagttacaa
    10261 acccggctcg cggtaaaaac aaatctgagt gcgcttatgg aggaaagaac ggagaccgtc
    10321 acccgtcgcc gtcggcagag tggtttatgg ggaaagattt gtggagcgtt tggcaccagt
    10381 gactggggct gggaaaccta taaagaggat gtgagccgca gtgttatcaa tatcaacacc
    10441 gtcaggaagg aagttatgtc actgacccgg gcatatttcg gggagctgca ggcatccatt
    10501 gagcaggata ttaaccagcc cgtccgccag gaaatcgatg cctttttctg cgcattcagg
    10561 gagaaagttg aacaactgcg taacacgctg attcagagct ctgaagatca taaacgcgat
    10621 cagcaggcac aggaacggct taccaggcga cttcaggcat taaatgaaag ggttcctgag
    10681 ctcattactg acagtaaggc gctgagggaa gagctggaga caatgctgtg acttcaccat
    10741 ttattcaaca aattgctgat aaccgggtat gtcaggtact cacctgtctt cctgaaaaat
    10801 ttgtggttga tttcgcaaac ggaatcgatg ttgcacagga gcatatccgc acggccggag
    10861 agcgcacgtt cttccggcgt ttaaaagagg gactgaccgg cgaaggcgct gcacggcaga
    10921 atgccatcaa tgcttcggtt gcacagggcc ttgaggcgtc cctgcgctgg ctgacggagc
    10981 tgacaacgtc acttgccacc acaaactacg cgattacccg ggtaaacgac agggtcagtt
    11041 cactggtcag tgatactgcc aggctggcgc attattccgc agatacacgg gagcagttac
    11101 tcatcctggc cgatcaggtt caccataaac tgaatcatct tgaagaaaaa ctccaccgtg
    11161 ttgaccaggt tcagcgggcg cagttacatc ttgagcagat attctcatgg tggagcgccg
    11221 ggcgatatgc gtcattttcc cctgccggac gttgttatgt ggcgcttgaa gagcttcgct
    11281 ggggcgcgtt tggtgatgtg atacgtcagg gcgaaacagg ccaggttaac cagctactgg
    11341 atattctcag acataaagca ttaacgcaga tggcacagga gagtggcggt agtgcaaccg
    11401 tgcgtctgaa cactctggac tggcttggtg gtcagggccg ggagcaggca gataacgagt
    11461 ggcatgacgc gattaactgg ctgggagact ggtgcagtga agagcagcat ccggtgatct
    11521 ggtcaaccac gcaggctgca gaacacttac cggttcgcat gcctcgcctc tgctctgcag
    11581 aacgcctctc tgaaagtatg gtcgatgaaa tatttcagaa gggggctgca tgagtacaga
    11641 aatgaaaacg gggctggtgc tgtccggtgg cggggcggtg ggcgcttatc aggcgggagt
    11701 ggttaaggca ctggcagagt gtggtacaca gatcagcatg gtttcagggg ccagcattgg
    11761 cgcattcaat ggtgccatta tcgcggcctc tcccgatctg tcagaagctg ccgtacgcct
    11821 ggaggcgctc tgggatcatc tggggaataa tcaggtgctg tcggtaaacc gattggttta
    11881 cttttcattg ctgaaaaaat tgttccaggc aatgaacctc tgccagatcc ccggacgtgc
    11941 aggagcactg cttacgacgc ttcttcgcca tatatcgata ctcaacgggt ttgacaatct
    12001 gatggctcag ccgttgttgt cagatgagcc cctgacagcg ctgttggatc attatcttga
    12061 tactgatgct ctggcagacg ggctaccgct gtatgtgtcg ctgtacccca cagaaggggg
    12121 catgcaggat attattgact gcattcgtgc tgaactgggt gccggaacca cgaaaaacgc
    12181 cgtttttcag catatccaga gcctgccccg cggacagcag aaagaggctc tgcttgcgtc
    12241 agccgcgctg cccctgctgt tccgtccccg tgaggttcag gggacaatgt tcggtgatgg
    12301 tggtatggga ggatggcgaa atatgcaggg aaatacccct gtgacgcctc tggtcgatgc
    12361 cggatgcaat atggtgattg tgacgcatct gagtgacggt tctttatggg atcgccaggc
    12421 ttttccggac accacaatcc ttgagatccg tccccggaaa aggctgaaat atgcaggtga
    12481 tggtgacaac agcggcggtc tgctcagttt cacgttggca cataccgacg cctggcgtca
    12541 gcagggctat gaagacacga tgctgacgat ggagcatatc cggaaaccgc tggcagcacg
    12601 tcaggtactg tcccggtcag aaacggtgtt gcagaaaagt ctggagataa cggaagaggc
    12661 agatttggca ctgagaaacg cgatggcccg gattaaataa agacgcccca aagaaacgcc
    12721 accgcctgtg gtggtgtttt tcctggctgg tgttattgaa aatattttca gatatatatt
    12781 acctgagaga aagttttacc cctcacctgt caattcactc actggaaatt taattatgca
    12841 at
//
LOCUS       DAZFRG010000137        12809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFRG010000137 DAZFRG010000000
VERSION     DAZFRG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.137"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(353..439)
                     /locus_tag="ACHKUC_003123"
     tRNA            complement(353..439)
                     /locus_tag="ACHKUC_003123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Leu,seq:gag)
     gene            complement(454..786)
                     /gene="secG"
                     /locus_tag="ACHKUC_003124"
     CDS             complement(454..786)
                     /gene="secG"
                     /locus_tag="ACHKUC_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO6619338.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1014..2351)
                     /gene="glmM"
                     /locus_tag="ACHKUC_003125"
     CDS             complement(1014..2351)
                     /gene="glmM"
                     /locus_tag="ACHKUC_003125"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO6619339.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2344..3192)
                     /gene="folP"
                     /locus_tag="ACHKUC_003126"
     CDS             complement(2344..3192)
                     /gene="folP"
                     /locus_tag="ACHKUC_003126"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6619340.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3282..5216)
                     /gene="ftsH"
                     /locus_tag="ACHKUC_003127"
     CDS             complement(3282..5216)
                     /gene="ftsH"
                     /locus_tag="ACHKUC_003127"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO6619341.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5316..5945)
                     /gene="rlmE"
                     /locus_tag="ACHKUC_003128"
     CDS             complement(5316..5945)
                     /gene="rlmE"
                     /locus_tag="ACHKUC_003128"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO6619342.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVNQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6071..6364
                     /gene="yhbY"
                     /locus_tag="ACHKUC_003129"
     CDS             6071..6364
                     /gene="yhbY"
                     /locus_tag="ACHKUC_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO6619343.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6520..6996)
                     /gene="greA"
                     /locus_tag="ACHKUC_003130"
     CDS             complement(6520..6996)
                     /gene="greA"
                     /locus_tag="ACHKUC_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO6619344.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7244..8677
                     /gene="dacB"
                     /locus_tag="ACHKUC_003131"
     CDS             7244..8677
                     /gene="dacB"
                     /locus_tag="ACHKUC_003131"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6619345.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8718..9890)
                     /gene="cgtA"
                     /locus_tag="ACHKUC_003132"
     CDS             complement(8718..9890)
                     /gene="cgtA"
                     /locus_tag="ACHKUC_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO6619346.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9906..10871)
                     /locus_tag="ACHKUC_003133"
     CDS             complement(9906..10871)
                     /locus_tag="ACHKUC_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6619347.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLADPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(10998..11255)
                     /gene="rpmA"
                     /locus_tag="ACHKUC_003134"
     CDS             complement(10998..11255)
                     /gene="rpmA"
                     /locus_tag="ACHKUC_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO6619348.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11276..11587)
                     /gene="rplU"
                     /locus_tag="ACHKUC_003135"
     CDS             complement(11276..11587)
                     /gene="rplU"
                     /locus_tag="ACHKUC_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO6619349.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11846..>12809
                     /gene="ispB"
                     /locus_tag="ACHKUC_003136"
     CDS             11846..>12809
                     /gene="ispB"
                     /locus_tag="ACHKUC_003136"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO6619350.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALVGLAHIAVQR"
BASE COUNT         3153 a         3548 c         3027 g         3081 t
ORIGIN      
        1 agattttttc tttttaaatg atgcggccat tgcgttctca gtaattaaat aacattatca
       61 atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac aggggcagga
      121 tggtttatcg aggagtaaac atatccgtat tcgtaccgga aatggagttg acacaataaa
      181 gtgccaatta tgtcagcaga agggaaaagc ttataactaa ggcataccgg caccatcgtt
      241 tcaaggtacc agctacgagt aaaacaactg gacgagatac agatacctga caaccattcc
      301 tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctttctggaa tttggtaccg
      361 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
      421 ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt
      481 agccggagca gctggctgag tttgttcggt tttcgccggc gcactcaggt tttcccattc
      541 gctaccttta ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa
      601 taacgttgcc agcagcgctg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
      661 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
      721 cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc
      781 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
      841 gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt
      901 cgcaagggca attttattgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
      961 tcgctgagaa aaaaggcgac gatcgccgcc tttttagcca gttatctaac gctttaaacg
     1021 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
     1081 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg
     1141 ctacccagcg ccgcttcaac ttctgcggtc acagctttga ctgattcatg ctccagcgga
     1201 tcgccgctac ctgcggtgta acgcacgttt accaggattt gcgggaacat tttcatgccg
     1261 ctgcaaaggt cgtgcaggct catatgatta cgcgccatcg ccgccagcac ctgcaaacca
     1321 gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct
     1381 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
     1441 gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc
     1501 aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
     1561 tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg
     1621 ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
     1681 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg
     1741 atagcgataa cgttcgctcc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca
     1801 ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt tgggaacgtg
     1861 gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt
     1921 tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc ctcttctact
     1981 gcatccggta gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg
     2041 ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc
     2101 acggccggtg ttggcatcgg accagtgaag agtgcggaaa ggcccgcagc cgccagaccc
     2161 gcttccagtg ctgactccag catgtagcca gaaatacgcg tgtctttacc aatgatgatc
     2221 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
     2281 tcaggggtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
     2341 cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc
     2401 ttctacggtt tctttgacgt catgaacacg aatgatatgc gcgccttgca tagcggcaat
     2461 gaccgcacaa gccagactac cgctcagacg ctcggacggc cccacgttca gcagctgccc
     2521 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
     2581 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
     2641 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
     2701 atagcgattc acttctgcaa agacatcgtc atacttcggg gcttcctgca tggtctttgg
     2761 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagctg cctccagagc
     2821 ccctggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc
     2881 acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat
     2941 tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgctcc
     3001 tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt
     3061 cgcatgtttc accgcatcta tcagcgagtt atgcgtaccg ccatccgaaa aggaatcagg
     3121 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag tgccctgggc
     3181 aaagagtttc atggtgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
     3241 ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttatttgtc gcctaactgc
     3301 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
     3361 ggactaccat tatcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga
     3421 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
     3481 atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca
     3541 cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
     3601 tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc
     3661 gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat gttacgtgcc
     3721 aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag
     3781 atgatctctt ccgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga
     3841 cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
     3901 cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatt
     3961 gcgtgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag
     4021 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca
     4081 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
     4141 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat
     4201 ggtacgcgac gcatgtgaac cttcaggatc tgctcacgac cgcgaacatc tggcaagcca
     4261 accacaacct gacggtcgaa acggccagga cgcagcagcg ctgggtcgag aacgtccgga
     4321 cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc
     4381 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc tgcgccacgc
     4441 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc
     4501 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
     4561 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc
     4621 gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt
     4681 ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc ttcttttgct
     4741 tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttcagt cagcatgcgc
     4801 gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
     4861 aagaagatcc agacaccaat cagcaacaac atcgggaacc aggagatgaa gatagaagcc
     4921 agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta
     4981 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
     5041 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
     5101 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
     5161 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
     5221 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
     5281 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
     5341 atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta
     5401 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
     5461 acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga
     5521 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
     5581 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
     5641 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
     5701 caagcgatga tgcggccttt gccgccaatt tggttgacca catattgtga ccaaccaccc
     5761 ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
     5821 atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca
     5881 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
     5941 gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
     6001 ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc
     6061 aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc
     6121 gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat
     6181 tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga
     6241 aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt
     6301 catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg
     6361 ctaagattat cctaaagtta cacacattcg ctgtgtaaaa cgaggggttt tccgcaggca
     6421 ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt
     6481 tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat
     6541 tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt
     6601 gccgatcagg ccacgagcaa taggcgagtt tacggaaatc aggttttgtt taaagtcagc
     6661 ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatcgagat tcagcaccgt
     6721 tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg
     6781 cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcaaaagc cctgctgttc
     6841 acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat
     6901 agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt
     6961 ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct
     7021 gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg
     7081 gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt
     7141 ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc
     7201 gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt
     7261 tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta
     7321 cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc
     7381 ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt
     7441 gattactgca ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct
     7501 tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg
     7561 tgccgatccg acgttaagac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc
     7621 tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga
     7681 taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc
     7741 cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga
     7801 tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac
     7861 cctcccccgt ggttctgccg aagcgcaata ctgcgagctg gatgtggtgc caggcgacct
     7921 gaaccgcttt acgctgaccg gatgcctgcc acaacgttct gagccgctcc cgttggcttt
     7981 tgccgtgcag gatggagcca gctatgccgg tgcaattctg aaagatgagt taaaacaggc
     8041 aggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt
     8101 agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc
     8161 ggacaacatg atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc
     8221 tggaacatgg cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt
     8281 cgatattgga aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc
     8341 ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt
     8401 tatctccatg ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca
     8461 tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa
     8521 cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc
     8581 tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga
     8641 aagccgtttg tataaagata tttatcagaa taattagcct aaagagaaac cccggcacat
     8701 ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg
     8761 tcccagtcgt catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctgacgg
     8821 tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg
     8881 accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtccg
     8941 ctcgccgcag agatcagata atatttatct tcccaaccca gcgcctcagc gatcgctttc
     9001 gctttctctt cggcttctgc cttatccagc aggtcgatct tgttgaagac taaccaacgc
     9061 ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt
     9121 tcaaccggat cggtgccatc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa
     9181 cgttccaggt gcttcaggaa gcgaatgccc agacctgcgc cttctgcagc gccttcaatc
     9241 agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccatacgcac cacgcccagg
     9301 cttggtacca gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgca
     9361 cgaataaagg tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc
     9421 atcagctcca gcagcagctc acgcttatcg cccggcgtac cgttagtttt ctgccgtggt
     9481 gtacggttaa ctgacgactt gaaacgggta ttgcccagac cgtgccagcc gcccttagca
     9541 accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccggt accctggtcg
     9601 attacacgcg tacctaccgg tactttaatc gtgacgtctt taccgcgctt accggtacag
     9661 tctcggcttg caccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg
     9721 ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccaccatc accgccgtcg
     9781 ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta
     9841 ccgccatcac ctgcaacgac cagaatcgat gcctcatcaa caaacttcat tttattctcc
     9901 gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc
     9961 caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga
    10021 taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga
    10081 tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc
    10141 tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa
    10201 aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg atctgccaga
    10261 gggaagagcg caattgtaca taaagtgtac agtaaaaaca ggatctgcgg tgaggccagc
    10321 cgacgcaata aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc
    10381 ccaaagataa ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg
    10441 ttaaaaaaca tcaccagacc gctcaggagc atcaatgccc ctacaacctg agtactgcgc
    10501 attttctctt tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca
    10561 atcacctgcg aagcggtcgg actcaagtat tgcaaggatg agctgaacag gatgaagttc
    10621 ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg
    10681 cgtaatggcg gcaaccgctt cttcaccgca agaatggcac caaggccaat actcgccatc
    10741 aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg
    10801 attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc
    10861 tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc
    10921 atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt
    10981 accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct
    11041 ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa
    11101 cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc
    11161 agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg
    11221 ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg
    11281 cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat
    11341 agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa
    11401 cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt
    11461 ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt
    11521 ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg
    11581 cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa
    11641 tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca
    11701 atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt
    11761 acaatcaccc tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat
    11821 cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca
    11881 agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccaactgat
    11941 caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc
    12001 tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct
    12061 gatcgagttt atccacacgg cgactctgct acacgacgac gttgtggatg aatcagatat
    12121 gcgcaggggt aaagctaccg ccaacgccgc atttggcaat gccgccagcg tgctggtagg
    12181 cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggttcac tcaaagtgct
    12241 ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa
    12301 cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctaca gcaagactgc
    12361 gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggagga
    12421 ggagaaaggc ctacaagatt atgggcgcta tctcggcact gctttccagt tgatcgacga
    12481 tttactcgat tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg acgatctgaa
    12541 cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc
    12601 acagatgatt cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct
    12661 ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc
    12721 agacaaagcc atcgcagcgt tacaggtgct cccggacacc ccgtggagag aagcactcgt
    12781 cggcctcgcg cacatcgctg ttcaacgcg
//
LOCUS       DAZFRG010000138        12801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFRG010000138 DAZFRG010000000
VERSION     DAZFRG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.138"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..783)
                     /locus_tag="ACHKUC_003137"
     CDS             complement(79..783)
                     /locus_tag="ACHKUC_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6619351.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(770..1606)
                     /locus_tag="ACHKUC_003138"
     CDS             complement(770..1606)
                     /locus_tag="ACHKUC_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6619352.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(1599..2432)
                     /locus_tag="ACHKUC_003139"
     CDS             complement(1599..2432)
                     /locus_tag="ACHKUC_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6619353.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMTADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(2432..3481)
                     /locus_tag="ACHKUC_003140"
     CDS             complement(2432..3481)
                     /locus_tag="ACHKUC_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6619354.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(3581..5149)
                     /locus_tag="ACHKUC_003141"
     CDS             complement(3581..5149)
                     /locus_tag="ACHKUC_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6619355.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(5524..7671)
                     /locus_tag="ACHKUC_003142"
     CDS             complement(5524..7671)
                     /locus_tag="ACHKUC_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7252..7254),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO6619356.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(7869..9335)
                     /gene="mdtP"
                     /locus_tag="ACHKUC_003143"
     CDS             complement(7869..9335)
                     /gene="mdtP"
                     /locus_tag="ACHKUC_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO6619357.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDASALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQTAPVVEKK"
     gene            complement(9332..11383)
                     /gene="mdtO"
                     /locus_tag="ACHKUC_003144"
     CDS             complement(9332..11383)
                     /gene="mdtO"
                     /locus_tag="ACHKUC_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO6619358.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     VSEINKLQHTIAEGQCWQSDWRLSEDEAVAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(11383..12414)
                     /gene="mdtN"
                     /locus_tag="ACHKUC_003145"
     CDS             complement(11383..12414)
                     /gene="mdtN"
                     /locus_tag="ACHKUC_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO6619359.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASSVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(12433..12708)
                     /locus_tag="ACHKUC_003146"
     CDS             complement(12433..12708)
                     /locus_tag="ACHKUC_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO6619360.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         3061 a         3409 c         3367 g         2964 t
ORIGIN      
        1 tataaagtgt tcgggtggac aaaaaaacaa ctgtaaaaag acctgaaagc agaatgaatt
       61 aatgcaatgt caggtttttt aatccatccg atgttccccc ttttccatgg cgtcgcgatc
      121 aaaaaatcgc tgaatttttc cctcggacat caatgccgct cgatcagaca tatgcgcgat
      181 gacatcggca tcgtgactga ccagcagata tgtcatttgg tgttccagct taagttgatt
      241 caacaaatta aggatttccg cttgtaccga catatccaac gccgaggtgg gttcatccag
      301 cagaagaagt tgtggtcgta acaacagggc gcgagcaata accacacgtt gccgttgacc
      361 accggaaagc tggtgtgggt agcgacgagc ggcgtcgaaa ggcaagccaa cctgttccag
      421 tgcagtctgg acgcgttttt ctatttctct ttcgccacga attttgagtg gttcagccag
      481 cgttcgccac agagtatgat tgggatgcaa cgaggcatag ggatcctgaa acaccatttg
      541 cacattgcgg cggagatcgc cctgaaaacg cattcctggt ttgatggtgt gcccaaacag
      601 tgacacctga ccgtgccagt cgcgctgtaa tccagcaatt acgcgcaaaa tcgtggattt
      661 tccgcagcct gattcgccaa ttaaactaaa tgtctctccg gcattaaccg aaaaactggc
      721 ggcagaaact gcggttttct cagcaaaagt cacctgtaag tgattaattt caacaatcgt
      781 catggtattt ctccggcgtc attgccgtcc tgtccagcgt cggcagcatc tgcccgtaag
      841 tttgtgcatt tgggcggcaa gtccacagtg tgcgcgtgta aggatgcgtt gctgtaggta
      901 gcgcagcagc gtgcatttca tccaccttat ttccctgata catcaccagc acccgatggc
      961 aaaattgggc aactaatggc aggtcgtgac tgatcagcaa cattgccatc tgacgttgtt
     1021 cacactgggt aaccagcagt tccagaatct gattacgcag gcgcacatcc agtgctgatg
     1081 ttggctcatc ggcaatcaat acccggggat cgttgatgag cgcaattgca atcatcactc
     1141 gctgccccat gccgccagaa agttcacgag gatagctttg cagtactcgc gtattgaggc
     1201 cgacagcctg aattgcggtg tttattttct cttctttctc gcggcggttc agacgttgat
     1261 ggagcgtaag cgcctcttcc agttgcgctt ttactgtctt tactggatta agcgcgtaac
     1321 gcggatcttg caataccatc gcgatatcgt taccgcgcaa ctgttgccat ccacgcgcgt
     1381 taagggtgag tgcatcacgc cccagaatgt taagtgtgtc cgcggttact acacccggtt
     1441 tacgcaccag ccccatcagg gcgcgggcgg tcatggactt acctgaaccc gactcaccga
     1501 ccagcgccag acgttcattg cctaatgtaa aactgacatt gttgacgacc cgggactccg
     1561 gataatcgac gcagagattg ttgatactaa gacgtgagtc agtcatgttg aggctccaga
     1621 acatcgcgca agccatcacc cagcagatta aaggccagac tggcaattaa gataattgcg
     1681 ccgggagcgg cggcaatcca ccattgatca aaaatgacct gcataccgtc ggcggtcatg
     1741 gctccccatt ccgccattgg cgggcgagca ccaagcccaa gaaaacccag gccagcagcc
     1801 gccagaatga tacccgctaa atccagcgcc agacggacaa tggcagatgg caggcacaat
     1861 ggcaaaatat gaccagccag cagacgcctg ccgcggatgc ccatcatttc tgcggcggca
     1921 agataatcac tgtgtcgcag acgttggatc tcactccgtg cctgacgcgc ataagcaggc
     1981 caggtggtta atgccagcgc cagtgcgccg ttaaccagcc ccggccccag catggcaaca
     2041 aaagcaaaag ccagaattaa acgcggcatt gacattacta cgtcagtaaa acgcatcagc
     2101 acgcgttcca gccagccgcc gtagtacccg gctaaaatgc caatcagcag accgaccggg
     2161 agtgtaatga ttgtcaccaa tgccaccagc cccagtgccg gacggctgcc gtagattaaa
     2221 cgtgaaagta ggtctcgccc gtaactgtca gtgccgagcc agtgctgacg actcggtgct
     2281 tgtaaccgcg ccgccgcatc ctgccagtta ggatcgtgag gtgccagcca cggcgcgaag
     2341 agcgcaatta gcatcaataa caggataatt atcaacccgc aaaacgcggc gggtgaatgg
     2401 cgtaaacggc gtaaaaagag aaacatcggc atcagcgtaa cctcggatcg gtcagccgga
     2461 ccagcaggtc ggttaagtta tttatcaaca caaagcagac gccaatcagc agcgttcctc
     2521 ccatgattgc tgtggtatca cccgcgaaga gcgctgtagt gaggtagcgc ccaatacctg
     2581 gccatgaaaa gacggtttct gtcagtaccg cgccttccag cattgatgta taggcgaggg
     2641 cgataacagt gagcaaggtg ccgcgaatgt tgggtaaaac atgacgtagc aaaattgtca
     2701 tctcgctggc tcctttggcg cgcgccagca gaatatattc tttattcatt tcgccaagac
     2761 aggcggaacg agtcaggcgc gtaatactgg cgagcgagaa gtacgccagt aataaaaccg
     2821 gaagtatcaa atggttgatt gcattggtaa atgcctcgcg atcgccagaa agccacgtat
     2881 caatcagcgc aaatccgcta cggggttcta tggtgaattg ataaatatca tccagcctgc
     2941 ccggcccggc actccattgc agtctggcgt agaacagcgc cagcatcagc agaccaagcc
     3001 aaaaaacagg gacggaattt cccagtagag ttaatgttcg cacggtgaaa tccagcggag
     3061 aaccgacata acgggcgcaa agtacacccg caatgacgcc gacaacagca ccgacaataa
     3121 gcgccaacgt tgccagttca atggtcgccg ggaatgctga tagcaagtcc tggagtactg
     3181 gttgcccggt ggagctggcg atgcccagat cgccgtgtgc cagattcatc aggtaatgcc
     3241 agaactggac ggctaacggc tgatccaatc ctaactggtg gcgtacctga tcataggtgg
     3301 actggctggc gtgatcgcca acgatttgta gtacgcgatc gaccggtgag agagccgaaa
     3361 gcgcaaaggt aatcagcagc aggccgagta gcgtcagagc aacggtaaac agcccttgca
     3421 acagcgcgag cagagggcgc ttgcctcgcc atctcgcgcg agtggaaaaa acgacagaca
     3481 tgatctttcc atacagtgat aaatcagcgg ctggcctgca atacaggcca gccctggtac
     3541 cgcgcccggt aacatggctg gacgcgagtt gcatacaggg ttatttggtt acctgatcaa
     3601 accagaccat atcggcattc aacccttgtt gatacccctg cacattatca cgcatgacaa
     3661 tttgcgtttt gccttgatca ataaagacat acggggaact acgttgtagt tcttgttgca
     3721 ttttcttata caaatcaaga cgtttagtgc tatcaggttc agcgatggcc gccagcgttt
     3781 ctttgttcag ttccgggatt ttccagccgt ttaacccggc aacggtgctg gatttgccat
     3841 cgttataggc gaaagagctg gcgttggaat gtgcgtcaaa ataatcaggc aaccacatac
     3901 gaatggcccc ctgatgctgg tgggcgcgaa cgcgagagta gacctgactc cctgcggctg
     3961 gcagcaaatc aacttttaca ccgccctgag caaagctggc ctgaattgac tgcgcgatgg
     4021 taataaatgg cggtttattt tcgacatcaa gcgtgaagtg cgggtctttg attccggctt
     4081 tagcgaggat ctcttttgct ttggctggat taaaggtaaa cggtgtttct tccagtgctc
     4141 caggaaagcc cacgggcagg aaactttgat gggtaaaata ttgtcctttt aagagatttt
     4201 tggtgatgcc gtcgtaatcg accaaccagc gagcggcctc ccagaacgca ggattactca
     4261 tcagcgggtt atccttgttt ccggtattga aggccaggta gttctgttcg gcggaggcaa
     4321 tgctcatcac ttttacgcca ggtttaccct gtaatgcatc gatttgatcg gctcccaggt
     4381 cacgagccat atccgcatca ccttgttgga tcagcaggcg acgtgttgcc ggatcgggaa
     4441 catttttgat gatgacgctt ttcagtttcg gggcgccggt tggtgagctg gcgttggctt
     4501 ccatcacgat ggcctgatgc ggttggtaag tacgcatttt aaacgcgccg ctgccagcgg
     4561 aatgcatctt cagccattca ttaccaaagt cgttattttt ggcgtttggc gcaaccagct
     4621 tctcgtcaac aattgaggcg attggtgtgg agagaatatt cagtgccagc gccgggctga
     4681 cgtcggcact ccattgtacc tggacggtat ggtcatcgat tttctttaac tggctggcga
     4741 tattttccgg ctgccatccc agcacattga gaataaacgc accggattta ttcagtgtga
     4801 cagcccggac ataagagtaa atcacatctt ccgggcgtac cggattacca gaggcaaatt
     4861 tcgcatcgct tttcagtttt atggttagcg ttttggctgc ggggtcagcc tgccaacttt
     4921 ccgccagaat cggagtgatt ttcgtcggat cattacggtc cggttgtact acgcgttgat
     4981 acagacttgg tacagtctga atacttgaga gttcattagc ttctgccggg tccagactca
     5041 caatatcatc cagcccctgg gctacaacca acgtattagg cggtgtagcg gcaacggttg
     5101 tggcggaaat tgtgcttaaa accagtaaag gtaatagttt tttaatcata tgcttcccaa
     5161 tccctgaaat tactttgagg ttattaatat gctatcagac ggggtttcgc tgcattaatc
     5221 gcgccagaat atcccgttaa tagaacgagt taaacggtcg atgatttctc accttaccga
     5281 aattttgttt atcaaatgta aagtattatt acgttaggcc gcgatgagaa ttgagtaaag
     5341 ataattctca tttgcaatcg tgactggaat agaagaacaa ccgagaagac gtcgctaata
     5401 accaggatgg gctggtttga ctgcgcaggt atgtataaaa aaatccggaa acagagagtc
     5461 gtttccggat tgatacaaag gcgttgggta atatcgccgc ttttaacgtg agtttacgcg
     5521 gtattacgcc agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac
     5581 gtactgctcg gcggcgcgct gatcggcgat cggctcgacg cgcacggcgc agtatttgta
     5641 ttccggcgtt ttcgtaatcg ggcttaagtt ttcggtcacc agctcgttac aggcaccaat
     5701 ccaccactgg taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg
     5761 ggtgataatt ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatacccag
     5821 acgtttggcg tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag
     5881 tgccgcacag ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga
     5941 cagtaccatc gggtactcgt cggtgagttt gtcgattggc gctacccagt cgcaggtgaa
     6001 gaactgcgcc agaccgttcg gggtatcaaa cttctcttta aacagataag aagttccttg
     6061 atcggcatct gaagtatcgc ggcaaggcca ctgaatgaag cccagttcgc ccattttctc
     6121 gtaagtcgca ccgtagaaat ccgggcacag atgacgcaac tcgtcccaga tctcctgggt
     6181 gttgttgtag tgcatcggat aacccatacg agtggcaatt tcactgatga tttgccagtc
     6241 cgttttcaga tcccacttcg gctcaaccgc cttgaagaaa cgctggaagc cacggtcagc
     6301 cgcagtaaac acgccttcat gctcgcccca cgacgttgac ggtaaaatga catccgccgc
     6361 cgacgcggtt ttggtcataa agatgtcctg aacgataacc agttccagat cttcaaaggc
     6421 tttacgtact gccgacagtt ccgcgtcagt ttgcagcgga tcttcgccca taatgtacgc
     6481 cgctctcact tcaccatgcg ccacgcggtg cggcagctcg ctgatgcgat agccggtatg
     6541 agcaggcagg ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt
     6601 cacgtactga tagcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgcac
     6661 gttgttctga ccacgaaccg ggtttacacc cgcatgtggc ttaccgaggt taccggtcag
     6721 catcgcgagg ctggtcagag aacgcacggt ttccacgccc tgatagaact gggttacccc
     6781 catgccccac aggatggcgg cgctttctgc ctgggcatac atccgcgccg cctgacgaat
     6841 ctcactggcg ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat
     6901 tttacgatac tcttcaaagc cttctgtacg ggaagcgacg aacgctttgt cgtacagatt
     6961 ttcttcaata atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcaa
     7021 tgcaatgtgc atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat
     7081 tttcgccccg ttacgtttag cgttaattac gtgattcgcc acgattgggt gggaatccgc
     7141 cgggttatac ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct
     7201 cattgcgcca ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg
     7261 agcgcagcag tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata
     7321 gttggtttcg ttacccgtac cgcgcgagga gccggtcgtc tggatggcat ccggaccgta
     7381 cttctctttg atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac
     7441 aggttcgagt ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag
     7501 gatctgggta tcgttaatga agtcccagcc ataataaccc ttcagacaca gggtaccctg
     7561 gttggttttc ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt
     7621 gattttgcaa cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc
     7681 tccagttaat caaatcacgc atacgcgctc tcgactacag tatgcatctt ttatgccaca
     7741 ttttatttgg ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa
     7801 acaggctgtc gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg
     7861 tagacatttt attttttctc gacgacggga gccgtctgat acccgccgcc cagcgatttc
     7921 atcaactgaa tgctttggat cacccggcgg ctgtccagca tcagtaatga catctcctcg
     7981 gcaagcactg gcaaccgggc ttcggtggcc tgtaagcggc tggttaagcc gcgctgatag
     8041 gcggcctcgg ctgcgcgctg ggtgaagcgc gttgcttcta cgcgttcagc ctgcatctct
     8101 cgctcgtcgt tgagcgtttg cagacgtgtg ccgttgacgg caacgtcacg caccgcgttc
     8161 agtactgact ggttgtaacg ttcaatcatc atgttgctgg cggcgcgagt gccttcgaga
     8221 ttggcattca accgtccacc gtcaaacagc ggcaatttca gacccgggat gaagttgaac
     8281 tggcgactgg tttttttgaa taaggtatcc aggtggatgg agtccagacc gaaaaacgct
     8341 ttgatatcaa agctcgggta gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc
     8401 tgaacatacc agcgcatggc ttgcagatcc gggcgtctgg cgagcaactc ataagagagc
     8461 gttgccggaa tgccggtctg tactcgtggt aatgccaccg gtttgatctc cggcatatcg
     8521 ctggctcccg cgccaatcaa tgcacgcaga gattctcgcg tttcagtgat ttgccctttg
     8581 acggcagcaa tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact
     8641 tgcgcttcca gaccgtgcgc cactttactc tggtgcgctt tcaccgcgta atcaatcaca
     8701 tcgcgagttt gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc
     8761 tgcgctacgc ccgtggtcag cgatagctct actgctgcgg tttctgccag cgcggcatta
     8821 tgcgcgccaa tggcggcagc aaccgctgag cgatgcacac cccacaaatc gagatccagt
     8881 ccggcaaaca aaccgactgt ggcttccgta tagtacggcc cgtccatacc cagtgctgac
     8941 gcatccatcg cataagggct taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc
     9001 cctaacgctg cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct
     9061 ttttcttccc gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc
     9121 agcgcatcca gctgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag
     9181 ctggcaagat gaatatcatc ggccagtttg atttgttccg gtttgagctg ttgatgaggc
     9241 gcagaatcct tacgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg
     9301 cacaacagca gacgtgaaag ttgacgattg atcattgcgt ggctccttgt gcctgttccg
     9361 tggcgggcgt taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt
     9421 gttgctcctg ttttaataag gtcggcagga tggcctgcga ggcgggtgtt tcttcaagcg
     9481 ttatttgagg agaacggctg agtgcggttg ccagaccggc ggcgtatttc tccagggcgt
     9541 cggcaaactg acctgctctg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc
     9601 aggtggcatc ccaggcgtga agaatatcgc ggccctgacg aataaccgtt tgcgaacgtt
     9661 caatcagtaa tgcgcgttct tcgctgtcca gttgacgctc cagcaccacg cgttggcaca
     9721 tctcttcaca ggcattaaac gccgcatgca gacctatgcg aatttgcaga taagtgcgca
     9781 gagccgtgac ttcctgctgg cgtgggatcc gcattacttt actcaacata cccagcgcgc
     9841 cagccagttt ttgcggcagc gtgcgcgctt cgctttcagg ccagacaaag gtgtaaatca
     9901 ccgcggacac cacggtacca atgatgatac ccagggcgcg atcgcgaatt tccaccaggt
     9961 cgtacaccgg gccaaaaacg ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg
    10021 tgccgatata agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg
    10081 gtgccagcac aaacagcaat tcgacaatat tgtccagcca gggcatgacc aataacgtga
    10141 ataacagcgc cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt
    10201 acgacgaacc gacattggga ttagcgacga tcacgcaggt cagcatacag gtgtgaatgc
    10261 cttcccagtc cacgccgctg tagaaggtgt aacagatcaa acaggcgagc agcgttttta
    10321 ccgcgtagcg catatagtct ggattggtaa aggcatcggc gaccattgat ggcggcttgg
    10381 ctgcaggcgt aggcggcgta ttcgggtcca tctgacccag ttgcaataac gtctggcaga
    10441 tattctccag gttacattct cgtgccgcca ccgcttcatc ttcactaagc cgccagtcgc
    10501 tttgccagca ctgaccttcg gcaatggtat gttgcagctt gttgatttct gagactaatt
    10561 tttgccgaaa ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca
    10621 gcgtcgagta aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ctctgggttc
    10681 gccagttggc atcatccgcg aggcaaaaga cattgagttt ttgcagcgcc agcgcctctc
    10741 tttcaatccg cgtttcgggt agcggtgcga ggctgtccgt caggtggcta atggcatcat
    10801 caagccgatc attaagcgcc tgatgcatct gcgtaatggc acgactggga aaccacagca
    10861 cgccgattaa cgtcatcagc aaggttggat agaggccaac aacgatacac cacagcgtta
    10921 agcgcacgac cacttccgga tagtcgagca tggcggggaa ggtttgcccg taaatagcga
    10981 caatggcgac ggcgaaaaag accagtccca gccgatgggt gcgcatcaaa aacatgcagc
    11041 ccatcaggat cggcccggcg atgatcaatc ggatcaacgg ttcgccatat gaccatttgt
    11101 agatcaaaaa caggctgccg atctccagca ccgtggcaac cacaaacaag atcgcgacaa
    11161 atttggtgta aaacgcgttc gactgaatac cgtaaaacag cactgccagt gataacgcca
    11221 caaaagggat ctcaaaagtc atcgagatca gaatcaccag caggcagcct acccagagtt
    11281 gcatagtctg tggcacgcgc cctggtcgcc gctcgcttaa ctcttcatga aagaacgcca
    11341 gcagcctgac caccggtaat ggcagggagt tgagcgcgct cattattgtg gctcaagatt
    11401 agcgactgcc gaagcgccga tgcggaacat ttccgggtca ggtttatcaa ccatgatttt
    11461 gaccggaaaa cgctgggcaa cgcggaccca gttaatagac cgtgacactt tcggcaggcc
    11521 gcccagcacc aggccgccgt catccggtag cacgccgtag ccaatcgaat ccactttacc
    11581 ctcgaaggtt ttgccgctgt cgctcatcag gcgaatggtt gcgagtgtac ccgagcgaat
    11641 atttttcaga tcggtttcgc ggaagttggc aatcacgtac cagtgacgag tgtcgattag
    11701 agtgaaaata gggcgcatcg cagatgcaaa ctgcccgacg gaggttttga gggaaatgac
    11761 ccggccatca aacggcgcgc gaacggtcgc catttccaga tgcagtttcg tcaacgcaat
    11821 atccgcttcg actgccgcac gttgcgccac cagcgcatcc acgccgctga cggagctggc
    11881 ggctgactgc gcctgcaaca atacggcatt aaggtccgct tctgccgcac gctgtgcggt
    11941 tcttgcacgg tcaacctctt ccgcagaaac aaaaccttct cgcagtagcg gttcggtacg
    12001 gcgtaacgta tcgctggctt gtttcgcggc ggcacgggct ttttctaccg tggcattaac
    12061 cgagtcggca ccaaactgtt gtgcgtcaac gctacgctgg gtgagcataa tttgcttatc
    12121 cagcgccgcg agggaggctt cagctttcgc cagattggct tcgtacgggc gcggatcaat
    12181 gcggaacagc aaatcgccct gtttcaccgc ctggttgtcg gtgactgcca gttctacaat
    12241 gcgaccgctg acttccggca ccacatcaat ggtatctgct gacgcgtaag cgtcattagt
    12301 tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg ccaacgcaac
    12361 cactaacaga gcagggaatt tactgcgagg agcttttttc ggcgtacttt ccatgagcgc
    12421 gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag caaaaagggc
    12481 ggtataaata attccgacaa atgccagatt gatatttgtc cgctgaataa ttcgcctcgt
    12541 gatgagcgtc aaaataaggc tggcaatagc gcagaaaaac cagccgggat aataagcgcc
    12601 gatcaccggg atcgccggag ataatgagca tcccgatacc atgaagacgg gaattatcca
    12661 ggctgtcttt ttgagttgca tcgccatgcg agagagaact gttggcatat taaatacatc
    12721 ctgtctgctg cgaacaatat ctgtagtgaa tatcaataac cattgaaggc gtcagtatgg
    12781 ctattttctt gatgaataaa a
//
LOCUS       DAZFRG010000139        12628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFRG010000139 DAZFRG010000000
VERSION     DAZFRG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.139"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1111)
                     /gene="tyrA"
                     /locus_tag="ACHKUC_003147"
     CDS             complement(<1..1111)
                     /gene="tyrA"
                     /locus_tag="ACHKUC_003147"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO6619361.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVSDAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQSFQSESRVLLRQAND"
     gene            complement(1122..2192)
                     /gene="aroF"
                     /locus_tag="ACHKUC_003148"
     CDS             complement(1122..2192)
                     /gene="aroF"
                     /locus_tag="ACHKUC_003148"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO6619362.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            2402..2767
                     /gene="yfiL"
                     /locus_tag="ACHKUC_003149"
     CDS             2402..2767
                     /gene="yfiL"
                     /locus_tag="ACHKUC_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO6619363.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENTSQ
                     LHEVWRKGADENASTIRLN"
     gene            2916..3434
                     /gene="yfiR"
                     /locus_tag="ACHKUC_003150"
     CDS             2916..3434
                     /gene="yfiR"
                     /locus_tag="ACHKUC_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO6619364.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            3424..4650
                     /gene="dgcN"
                     /locus_tag="ACHKUC_003151"
     CDS             3424..4650
                     /gene="dgcN"
                     /locus_tag="ACHKUC_003151"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO6619365.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            4666..5148
                     /gene="yfiB"
                     /locus_tag="ACHKUC_003152"
     CDS             4666..5148
                     /gene="yfiB"
                     /locus_tag="ACHKUC_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6619366.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(5225..5572)
                     /gene="rplS"
                     /locus_tag="ACHKUC_003153"
     CDS             complement(5225..5572)
                     /gene="rplS"
                     /locus_tag="ACHKUC_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO6619367.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(5614..6381)
                     /gene="trmD"
                     /locus_tag="ACHKUC_003154"
     CDS             complement(5614..6381)
                     /gene="trmD"
                     /locus_tag="ACHKUC_003154"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO6619368.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(6412..6960)
                     /gene="rimM"
                     /locus_tag="ACHKUC_003155"
     CDS             complement(6412..6960)
                     /gene="rimM"
                     /locus_tag="ACHKUC_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO6619369.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(6979..7227)
                     /gene="rpsP"
                     /locus_tag="ACHKUC_003156"
     CDS             complement(6979..7227)
                     /gene="rpsP"
                     /locus_tag="ACHKUC_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO6619370.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(7364..8725)
                     /gene="ffh"
                     /locus_tag="ACHKUC_003157"
     CDS             complement(7364..8725)
                     /gene="ffh"
                     /locus_tag="ACHKUC_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO6619371.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            8892..9683
                     /gene="ypjD"
                     /locus_tag="ACHKUC_003158"
     CDS             8892..9683
                     /gene="ypjD"
                     /locus_tag="ACHKUC_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO6619372.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            9704..10990
                     /gene="yfjD"
                     /locus_tag="ACHKUC_003159"
     CDS             9704..10990
                     /gene="yfjD"
                     /locus_tag="ACHKUC_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO6619373.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQVLMMPLVWLLNAITRILMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(11045..11638)
                     /gene="grpE"
                     /locus_tag="ACHKUC_003160"
     CDS             complement(11045..11638)
                     /gene="grpE"
                     /locus_tag="ACHKUC_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO6619374.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKD"
     gene            11761..>12628
                     /gene="nadK"
                     /locus_tag="ACHKUC_003161"
     CDS             11761..>12628
                     /gene="nadK"
                     /locus_tag="ACHKUC_003161"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO6619375.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSK"
BASE COUNT         3122 a         3384 c         2932 g         3190 t
ORIGIN      
        1 tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaactctgt gcgtaatcgc
       61 cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg ccctgctcca
      121 gcagctcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc agattacgct
      181 ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt cgcccgacca
      241 tcgccagctc aaggcggtaa atcggcgaag agagcgccag tagttgctca agctgaacat
      301 tttcttctgc caggtgcagc ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa
      361 acgccatatt ctgatcgtgc tcgacagcgc taatacgatg caaccgagcg ccccagacct
      421 gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac cagaccacaa
      481 cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc agcaccgggc
      541 catcgtgcgc cgccagcatg gcctgtaatg gtccattttt caccgatgcc agatcaacca
      601 gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta acgtggatgg
      661 gcacactaac aatcaccatt ccggcatcgg aaacaatatc agccgctcga tcccagtcat
      721 gttgctccag aatccgcacc tgataacccg agagtgtcag catcttctcg aacagacgtc
      781 ccatctgacc gccaccaccg acgataacca ccgggcgcag tgacggacaa agcgttttaa
      841 atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa
      901 tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc aacatagatg
      961 cctcgcgctc cggaacataa ataggtagtc caaagcggct tttcacctcg cccacttcag
     1021 caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact tcatcaattt
     1081 gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac gcgagccgtc
     1141 agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat ttcccagcta
     1201 atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg ctcggaagac
     1261 tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg attgccatct
     1321 ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc tttattggaa
     1381 ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc catctctttt
     1441 tcacattgcg caacatccgc agggctatag tttggcgctt taccaccgcg caggatcaca
     1501 tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg gttaatgcca
     1561 acaaaacggt gcggctgggc ggcggcacgc atagcgttaa ttgccgttgc cagactgcca
     1621 tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc acggtgagtt
     1681 tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc caggtattgc
     1741 gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac cagctcaagc
     1801 agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc catatgggga
     1861 tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata gacgcgcatt
     1921 accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg agcatattcc
     1981 agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag acgaggatcg
     2041 cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc ttcttgttgc
     2101 aagcttaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg ttcgtcggta
     2161 atatgtacgt tattcagcgc gtctttctgc atgatggcga tcctgtttat gctcgtttgc
     2221 gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag ttttcaatcc
     2281 atatttagta catatttctt tacataagca atttattcgc gctttttccc cttatttctg
     2341 tataaataaa tttacaccag ctttaaaaac aatccgctat gctttgaaaa aggagaaaga
     2401 aatgatgaaa aaatttatcg cccccttgtt ggctttactg gttagcggat gtcagattga
     2461 tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg gtatggaaga
     2521 tgcgatatcg ggcagcgcca ttaaagatga cgatgcattt agcgattcac aggcggatcg
     2581 cggtctatac cttaaaggat atgccgaagg gcaaaagaaa acttgccaga ccgattttac
     2641 ttatgcccgg ggactttctg gtaaaagctt tcctgccagc tgcaataatg ttgaaaatac
     2701 aagccaactg catgaagtat ggcgaaaagg ggctgatgaa aatgccagca caatacgatt
     2761 aaattaatta cccccccaaa atcaattaag catgagattt tactcattat gttttatttc
     2821 tcacattgat gacgaccgca tgaaaggatg atattttaat gagcattctg aaaaaatatc
     2881 gatgcatttc gagcgaagat ggtgaagatc cctgaatgcg tttttctcac cgactgttct
     2941 tactgctgat tctcctgctg acgggagcac caatactcgc tcaggaacct tctgacgttg
     3001 cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg cctgggctat
     3061 cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc ttacaagaaa
     3121 atgctgctac atctttacct tatctgcctg tcatcattca tactcaacaa gaggcgataa
     3181 tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag atggaattaa
     3241 ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc gagtgcatta
     3301 ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc gtaaacctgg
     3361 atgccttatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc gcacggaaga
     3421 aaaatgatgg ataacgataa ttctcttcat aagcgcccca cgtttaaaag agcattacgc
     3481 aacatcagca tgaccagcat atttatcact atgacgctga tctggctgct gctttccgtg
     3541 acgtcagtgc taaccctgaa acaatacgcg caaaaaaacc tggcactgac agcagcaaca
     3601 atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc aactgaaaca
     3661 ctggcagcgc tgggccagca aggccaattt tcaactgcag aagtgcgtga taagcagcaa
     3721 aatattctgg catcctggca ttacacccat aaggaaccag gcgatacttt cagcaatttc
     3781 ataagccact ggctcttccc tgcccccatc attcagccga ttcgccacaa tggtgaaacc
     3841 attggcgaag tacgcttaac tgctcgcgac agttcaatca gccattttat ctggttttcg
     3901 ctcgctgtcc tgaccggttg tattctgctg gcatcaggca tcgcaattac cctcacccgc
     3961 catttgcaca atggcctggt ggaagcgctg aaaaatatca ccgatgtcgt acatgatgtg
     4021 cgttctaacc gcaatttttc ccgacgagtt tcggaagaac gtatcgctga gtttcaccgc
     4081 ttcgctctcg acttcaacag tctgctggat gaaatggaag agtggcagct tcgtttacag
     4141 gccaaaaatg cgcagcttct acgtaccgcg ctacatgacc cattaaccgg gctggctaac
     4201 cgcgcagcgt ttcgtagcgg catcaacacg ttgatgaaca attccgatgc ccgaaaaacg
     4261 tcggcgttac tatttcttga tggcgataat ttcaaatata tcaatgatac ctggggtcat
     4321 gcgacgggcg atagagtctt gattgaaata gcaaaacggt tagctgaatt tggcgggctg
     4381 cgacataaag cataccgcct gggcggcgat gaattcgcta tggtgctcta tgatgtacag
     4441 tcggaatctg aagtgcagca gatatgctca gcactgacac aaatttttaa tctcccgttt
     4501 gatcttcata atggccatca gactaccatg acattaagca ttggttacgc gatgactatt
     4561 gagcacgcct ctgcggaaaa tttacaagag cttgccgatc acaatatgta tcaggccaaa
     4621 caccagcgtg ccgaaaagct ggtgagataa caaggatata tcgatatgat aaagcacctg
     4681 ctagcacccc tgattttcac ctcgctaata cttactggct gccagtcccc tcagggaaag
     4741 tttactcctg agcaagtcgc cgctatgcaa tcttatggat ttactgaatc cgccggcgac
     4801 tggtcgctgg gcttatcaga tgccattctg tttgcaaaaa atgactacaa attgctcccg
     4861 gaaagccagc aacagatcca aaccatggca gccaaattgg cctcgacagg gctaacacat
     4921 gcccgtatgg atggacacac cgacaactat ggtgaagaca gttacaacga agtcttatca
     4981 ttgaaacggg cgaatgtcgt ggccgatgca tgggctatgg gtggacaaat tccgcgcagc
     5041 aatctcacca ctcagggttt aggaaaaaaa tatcccatag ccagtaacaa gaccgcccag
     5101 ggccgcgccg agaaccgccg cgtcgcagtg gtgattacaa ccccttaaaa cacgagcaaa
     5161 cagacaaaaa aagggccagc caattggcca gcccttctta acaggatgtc gcttaagcga
     5221 aatcttagtt aagacgctct ttgatacgag cagccttacc agtacgctca cgcaggtagt
     5281 acagtttagc tttacgaaca gcaccacgac gtttgacaga aatgctgtca actaccggag
     5341 agtgagtctg gaagacacgc tcaacgcctt cgccgttgga aattttacga acagtgaatg
     5401 cagagtgcag accgcggtta cgaatagcga taaccacgcc ctcgaatgcc tgcagacgtt
     5461 ttttggaacc ttcaacaacc catactttca cttccacggt atcacccgga cggaaggaag
     5521 gtacgtcctg cttcatctgc tcttgttcaa gttgcttaat aatgttgctc ataatttaat
     5581 ctcttatcct gggtaaactg atatctcggg ggcttacgcc atcccatcat gtttatgttg
     5641 ctgttgtgcg tgttccgttt tgaactccgc cagcaacctt gcttgctctt cagtcagagc
     5701 caggttttcc agaagttcag gtcttctaag ccaggtacgg cccagcgact gtttcaaacg
     5761 ccagcgacgt atctcggcat ggttgcccga cagtaacact ggcggaactt ccatcccttc
     5821 taacacctca ggccgcgtat agtgcgggca atccagcaat ccttcagcaa aggaatcttc
     5881 cgttgccgag gcttcatgtc ccagtactcc cggaataaac cgggaaacgg agtcaatcag
     5941 cgtcattgct ggtaactcac caccactgag aacgtaatcg ccgattgacc attcttcgtc
     6001 aatttcggtt tggatcacgc gctcatctat accttcgtag cgaccacaca ccagaatcaa
     6061 tttttgattc gttgccagtt cgctgacgcc cgcttgatca agcttgcgtc cctgtggtga
     6121 cagataaatc acctttgcgc cttcacccgc cgcggctttt gctgcatgaa tggcgtcccg
     6181 caagggttgc accatcatta acatccccgg tccgccgccg taaggacgat cgtccacggt
     6241 acggtgccgg tcatgcgtga agtcgcgagg actccagctc tggatgctca gcaggccatt
     6301 tttaactgcc cggccagtta ccccgtaatc ggtaattgcg cggaacattt caggaaacag
     6361 gctaattatg ccaatccaca tagcgccgtc ttttaccgtt tatccggtgg tttaaaaacc
     6421 aggatcccaa tctacttcga ttgaacgtgt agtgagatcg actttcttga taacctgccc
     6481 atcgaggaac ggtacgagac gttccttgat accaaacgca tctttcaggt ttgccttaat
     6541 gacgagaacg tcattagatc cggtttccat catatctacg actttaccga gatcgtagcc
     6601 ttcagtggtt actacctggc agcccatcag gtctttccag tagtagtccc cctcttcaag
     6661 ctgaggcaac tgcgatgaat ccacgacaat ttcacaattc gtcagcaggt tcgccgcatc
     6721 acgatcgtca acgcctttca gcttgatgat catgtcctga ttgtggtgct tccagctttc
     6781 cagctggact tgctgccact gacccgcctt ctggataaac cagggctgat agtcaaaaat
     6841 gctttcggcg tcttcggtgg aagaaaacac tctgagccac ccacgaatac cgtaagacga
     6901 ccccattttt cccaaaacga tgggatcaac aggtgcttgc gcggtgagtt gtttgctcat
     6961 catgaccacc gtgacagatt aagctgcttt gtttacttct ttgatcagcg cagcaacgcg
     7021 atcagaaata gttgcgccct ggccaaccca gtgagcgatg cgatccagat ccaggcgagt
     7081 gccttcttct ttttcgctag cgattgggtt gaagaaacca acgcgctcga tgaagcgacc
     7141 gttgcgtgca ttacggctgt cagcgacaac aacctggtag aacggacgct ttttagcgcc
     7201 gtgacgtgct aaacgaatag ttaccataac atcctcttgt gtgaataaaa caaccggacc
     7261 ccatcgagga acggagtccg gtgtcatatt aaaagcccga aaattttact gatttctgga
     7321 gaaaatgcaa tcagcagtta ataactctat cgcataaggt catttagcga ccaggaaatc
     7381 ccggcggcat catccctttc atgcttctca tcatcttcgc catcccgccc tttttcattt
     7441 tcttcatcat gcgctgcatg tcgtcgaact gtttcagaag acggttaacg tcctgcacct
     7501 gcataccgca accggcagcg atacgacgct tacgcgaacc tttgatgatt tctggcttag
     7561 cgcgctcttt catcgtcatc gagttgatga tggcttccat acgcaccagc actttatcgt
     7621 ccatctgtga cttgacgtta tccgggatct gccccatgcc cggcagcttg cccatcaggc
     7681 tagccatgcc gcccatattt ttcatctggc gcagctgctc aagaaagtcg ttgagatcga
     7741 agccgtcgcc ttttttcagc ttgctggcta atttctctgc ctgcgcgcgg tcaaccttgc
     7801 tttcgatatc ttcgatcagt gacagtacgt cgcccatgcc gagaatacgg gaggcaatac
     7861 ggtccggatg gaatggctcc agcgcctcgg ttttctcgcc aacaccgagg aacttgatcg
     7921 gtttgccagt gatgtgacga atagagagcg ccgcaccgcc gcgggcatcg ccgtccactt
     7981 tggtcaacac tacaccggta agcggtaacg cttcattgaa tgcttttgcc gtattggccg
     8041 catcctgacc ggtcatggca tcgaccacaa acagggtttc aaccggatta atcgacgcat
     8101 ggacttgttt gatctcgtcc atcatcgctt cgtcaacgtg cagacgaccg gcagtatcca
     8161 ccagcagcac gtcgtagaat ttcagtttgg cttctttcag cgccgcgtta acgatatcta
     8221 ccggcttctg accaacatca gaagggaaga aatcaacgcc cacctgctct gccagcgtct
     8281 caagctgttt gattgccgcc gggcgataga cgtcggcaga aaccaccagc actttcttct
     8341 tgtgcttctc gcgcaggaac ttaccgagtt taccaacgct ggttgtttta ccggcacctt
     8401 gcaggcccgc catcagcacg accgcaggcg gttgcgcagc caggttcagg gtctggttct
     8461 cttcgcccat cgccgcaacc agttcgttac ggacaatttt gacgaactcc tgccccggcg
     8521 tcaggctctt attaacttca tgaccaaccg ctttctcttt tacgcgattg ataaactcac
     8581 gcactaccgg cagagcgacg tccgcctcca gcagcgccat gcgcacttcg cgcagcgtat
     8641 cttttacgtt gtcttcagtg aggcgtccac ggccactgat attgcgcagc gtgcgcgaca
     8701 aacgatcggt taaattatca aacattgtct ctcgcctggg gtggaaacgg ttggccgcaa
     8761 tcgcgacaca tcatcagtat tttgccgcag tataacatga aggcgtcttt gttgttatgc
     8821 aacggttgga gcagcgttca cctgacgcta tactgcttct ctttcttatt gctcaaactg
     8881 tcgacattac tatgcccgtt tttgctctgc tcgcgcttgt cgcctactcc gtcagtcttg
     8941 cgctgattgt tcccggtctg ctgcaaaaaa acggcggctg gcggcgcatg gctattattt
     9001 ctgcggtcat cgcgctggtc tgccacgcaa tcgctctgga agcccgcatc ctgcccgacg
     9061 gtgatagcgg acaaaacctc agcctgctga acgttggttc attggtcagt ttgatgatct
     9121 gtacggtaat gaccattgtg gcttctcgca atcgtggctg gttgttgcta cccattgtct
     9181 atgcctttgc gcttatcaac ctggcgctgg cgaccttcat gcccaatgaa tacatcaccc
     9241 atctggaagc aacgccgggg atgctggtgc acattggctt atcgcttttt tcctatgcca
     9301 cgctaattat cgctgccctg tacgcgctgc aactggcgtg gattgattac caactgaaga
     9361 acaagaagct ggcgtttaac caggaaatgc ctccattgat gagtatcgag cgtaaaatgt
     9421 tccatatcac gcagattggc gtggtgctgc taacgctcac tctttgcact ggcctgttct
     9481 acatgcacaa cttatttagc atggaaaata tcgacaaggc cgtgctctct atcgtggcgt
     9541 ggtttgtcta tattgtgctg ctgtggggac attatcatga aggatggcgt ggacgccgcg
     9601 tcgtctggtt taacgttgcg ggtgcagtca ttctgacact ggcctacttc ggcagccgaa
     9661 tttttcagca gttaatcagc taaacccaga aaaggagttt cccctggaac acatttctac
     9721 tactacgttg atcattattc tgatcatcat ggtggtcatt tcagcctatt tttccggttc
     9781 agaaactgga atgatgacac tcaaccgcta tcgcctgaga cacatggcga agcaaggtaa
     9841 tcgctcggca aaacgcgtcg aaaaattact gcgcaagcca gaccgcctga taagcctggt
     9901 gttaatcggc aataatctgg tcaatatcct tgcttccgca ctcggcacta ttgtcgggat
     9961 gcgcttgtat ggcgatgctg gcgtggcaat tgcgactggc gtgctgacct tcgtagtgct
    10021 ggtttttgct gaagtattgc cgaaaaccat tgccgcactg tacccggaaa aagtcgccta
    10081 tccgagtagt tttctactgg cgccgctaca agtcctgatg atgccgctag tctggttact
    10141 aaatgccatc acccgaattt tgatgcgcat gatgggcatc aaaaccgata tcgtggttag
    10201 cggctctttg agcaaagaag agttgcgtac catcgtgcac gaatcacgct cacaaatttc
    10261 ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg gaaaagatga ccgtcgatga
    10321 catcatggtg ccgcgcagtg aaattatcgg tattgatatc aacgatgact ggaaatcgat
    10381 tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg ctctaccgtg attcgctgga
    10441 cgacgccatc agtatgctgc gtgtgcgtga agcctggcga ctcatgtcgg agaaaaaaga
    10501 gttcaccaaa gaaaccatgc tacgcgccgc ggacgagatc tattttgtcc cggagggtac
    10561 gccgctcagc acacagttgg tgaagtttca gcgcaacaaa aagaaagtcg gcctggtcgt
    10621 caacgagtat ggagacattc agggactggt gacggtcgaa gatattctcg aagagattgt
    10681 cggcgatttc accacgtcga tgtcgccaac acttgccgaa gaggtcacgc cgcaaaacga
    10741 cgggtcggtg attatcgatg gcaccgccaa cgtgcgagaa attaacaaag cctttaactg
    10801 gcatctaccg gaagatgatg ctcgtactgt taacggcgtc attctggaag cgctggagga
    10861 gatcccggta gcaggcaccc gcgtgcgtat aggcgagtac gatatcgata ttctcgacgt
    10921 acaggacaat atgattaagc aggtaaaagt ttttcctgta aaaccgctgc gcgagagtgt
    10981 ggcggagtaa cgaaaacggc ccggcattcg catgctgggc cgtgatttat tacgaaagca
    11041 gaaattaatc ttttgctttc gctacagtaa ccatcgccgc acgaatcgta cgaccgttca
    11101 gcgtataacc tttctgcata atgcccagta cgttacctgg cgcaacgtca tcagattcca
    11161 ccattgcgat ggcctgatgc acatttgggt ccagtgggac gttggtttcg gcgatcactt
    11221 caacgccaaa cttacgcaca acatccagca tcgacttcag cgtcagctca ataccttcaa
    11281 ccatcgcgga catatccggg ttagctttat cagccacttc cagcgcccga tccaggctat
    11341 caatcaccgg cagcaattcg ttgatgaatt tctccagcgc gaatttgtga gctttttcaa
    11401 tatccagttc agtacgacga cgcaggtttt ccatttcggc ttttacgcgc aaaatgccgt
    11461 cacgttcacg ggtctgggct tcagccagct gagcttcgag attcgcaatt ttttcatcgc
    11521 gcggatccac ctgctcagca gaagcttctg gctcaactgc ctcaatctct tcgtgctgat
    11581 ccatgataat ttcttccggg gcttgcccct caggcgtttt ctgttcttta ctactcatga
    11641 atttctccgc gtttttttcg cattcatctc gctaacttcg cttattatgg ggatcagttt
    11701 cagggtttca agggaagcac tcacattgtc atcaatcttc gcaacaagga cctcggaaaa
    11761 atgaataatc atttcaagtg tattggcatt gtgggacacc cacggcaccc cactgcactg
    11821 acaacacatg aaatgctcta ccgctggctg tgcacaaaag gttacgaggt catcgttgag
    11881 caacaaatcg ctcacgaact gcaactgaag aatgtgaaaa ctggcacgct cgcggagatt
    11941 gggcaacagg ctgatctcgc ggttgtcgtt ggcggcgacg gtaatatgct gggcgcggcg
    12001 cgcacgctcg cgcgctatga tattaaagtt attggaatca accgtggcaa cctgggtttc
    12061 ctgactgacc ttgatcccga taacgcccag caacaattag ccgatgtgct ggaaggccac
    12121 tacatcagcg agaaacgttt tttgctggaa gcgcaagtct gtcagcaaga ttgccagaaa
    12181 cgcatcagca cggcgattaa cgaagtggta cttcaccctg gcaaagtggc gcatatgatt
    12241 gagttcgaag tgtatatcga cgagatcttt gcgttttcgc agcgttccga tggactgatt
    12301 atttcgacgc caacaggctc caccgcatat tccctctctg cgggcggtcc tattctgaca
    12361 ccctctctgg atgcgattac cctggtgccc atgttcccgc atacgttgtc agcacgacca
    12421 ctggtcataa acagcagcag cacgatccgt ctacgttttt cgcatcgccg taacgacctg
    12481 gaaatcagtt gcgacagcca gatagcactg ccgattcagg aaggtgaaga tgtcctgatt
    12541 cgtcgctgtg attaccatct gaatctgatt catccgaaag attacagtta tttcaataca
    12601 ttaagcacca agctcggctg gtcaaaaa
//
LOCUS       DAZFRG010000140        12542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFRG010000140 DAZFRG010000000
VERSION     DAZFRG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.140"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(129..1004)
                     /locus_tag="ACHKUC_003162"
     CDS             complement(129..1004)
                     /locus_tag="ACHKUC_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6619376.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            complement(1065..1967)
                     /gene="yihR"
                     /locus_tag="ACHKUC_003163"
     CDS             complement(1065..1967)
                     /gene="yihR"
                     /locus_tag="ACHKUC_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO6619377.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            complement(2034..3275)
                     /gene="yihS"
                     /locus_tag="ACHKUC_003164"
     CDS             complement(2034..3275)
                     /gene="yihS"
                     /locus_tag="ACHKUC_003164"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO6619378.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(3291..4169)
                     /gene="yihT"
                     /locus_tag="ACHKUC_003165"
     CDS             complement(3291..4169)
                     /gene="yihT"
                     /locus_tag="ACHKUC_003165"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO6619379.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(4193..5089)
                     /gene="yihU"
                     /locus_tag="ACHKUC_003166"
     CDS             complement(4193..5089)
                     /gene="yihU"
                     /locus_tag="ACHKUC_003166"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO6619380.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5257..6153
                     /gene="yihV"
                     /locus_tag="ACHKUC_003167"
     CDS             5257..6153
                     /gene="yihV"
                     /locus_tag="ACHKUC_003167"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO6619381.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNEAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHEGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            6187..6972
                     /gene="csqR"
                     /locus_tag="ACHKUC_003168"
     CDS             6187..6972
                     /gene="csqR"
                     /locus_tag="ACHKUC_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6619382.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            7071..7670
                     /gene="yihX"
                     /locus_tag="ACHKUC_003169"
     CDS             7071..7670
                     /gene="yihX"
                     /locus_tag="ACHKUC_003169"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO6619383.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            7664..8536
                     /gene="yihY"
                     /locus_tag="ACHKUC_003170"
     CDS             7664..8536
                     /gene="yihY"
                     /locus_tag="ACHKUC_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO6619384.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            8533..8970
                     /gene="dtd"
                     /locus_tag="ACHKUC_003171"
     CDS             8533..8970
                     /gene="dtd"
                     /locus_tag="ACHKUC_003171"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO6619385.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDADGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9015..9956
                     /gene="fabY"
                     /locus_tag="ACHKUC_003172"
                     /gene_synonym="yiiD"
     CDS             9015..9956
                     /gene="fabY"
                     /locus_tag="ACHKUC_003172"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO6619386.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10020..10928)
                     /locus_tag="ACHKUC_003173"
     CDS             complement(10020..10928)
                     /locus_tag="ACHKUC_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6619387.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            11157..11468
                     /locus_tag="ACHKUC_003174"
     CDS             11157..11468
                     /locus_tag="ACHKUC_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6619388.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            11469..11759
                     /gene="nadS"
                     /locus_tag="ACHKUC_003175"
     CDS             11469..11759
                     /gene="nadS"
                     /locus_tag="ACHKUC_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6619389.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            12118..12396
                     /locus_tag="ACHKUC_003176"
     CDS             12118..12396
                     /locus_tag="ACHKUC_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619390.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
BASE COUNT         3088 a         3099 c         3285 g         3070 t
ORIGIN      
        1 agctgtgggc gtggcgtatc cataaataat ctccatagat ataaaactaa tatttcatgc
       61 catcctgtta ttacaggttt cattaattaa agaatgtgtg cgggtataaa ttaaaacccg
      121 cataatgatt atttactttc ttccgcccga cggattgccg ccactttttt accaaatgcc
      181 agttgcagta taactaacaa ggtaaacgga ggaagaagtg cggcagcggc gggaattccc
      241 agcgcgcctt ttatcaacat agaaccggag gcaatcagca ataaaaagac catcacacgg
      301 aacactttga ccgaaatgcg tttatgcaca tattgtccaa ccagtgaacc gaggatcatg
      361 gtgggaatac aaaacagcac cagtttaaag atagagacag tcaaaattcc gctggtcgcc
      421 agcccgccga tcaccgcgat gttgtttgcg gtgaagaaag cgttcagagt gccgcgaaat
      481 gccgccgggc ctaagttacg cagcataccg tagataacca tcggcggtcc gttggttgaa
      541 aatgctgccc ctaacgctcc tgccacggcg ccgataggtg cggcgatcca gctacggtca
      601 taaatcggca ggcgtggaat aaacatactg taaaaggagt acaggataag gaatccgcct
      661 aagcccactt tcataaaatg gtcgggtaaa tacgacagcg catataaacc gacgggtatt
      721 cccagaaacg agaaaataat cagtaccatt gccgatcgcc aatccgtttc tttacgtgac
      781 agccaggtgg cgtacaaggc ggtcgcggtg ccgacaatca ccgagagcgg agttgccatt
      841 tttacgggga gcattaacgt gacgagcggc atggtggtta aaccaccgcc aaatcccgcg
      901 catatcccca caaaggtata aagaaacatt aacgcgataa cgatacatac cacgtttgtt
      961 tccagcatga ccccatccgg tgaaaataac gtaaaaaagt ccataatatt tcctgttgtg
     1021 taaatacaac tctaaacaat gatctcgttc ttttcttaat aaaattaatt gtgctgcccg
     1081 tgaatattaa aaaacaggcg gtgcgttttc cctggttcca gtaataataa gtcgatacct
     1141 gaattaaagg cgttaggcgg gcaactcatt ggttcaactg ccagtccctg acgttgtaat
     1201 ttttcaccgc tataaacctg cacccagggt tgatcggaac agaggctcac cgaaagcgcc
     1261 tgctgtggat gggtaatagt catttcccac aaatcattag cggttttaaa ggtgtgatcg
     1321 atttttgtcg cggctatttt tttcgcctgc gtgaaattta aatccagctc atcaacgtgg
     1381 tgaagcgttg tcggattagc gtgctcatcg atggcataaa cttggtttgc cggaagctgg
     1441 aacagatatt catcaacaga tgttaagttg caggttaaat aagggtgaat acccacaccg
     1501 taaggcgctg cgacagtgcc gatattctgg cttgcgatct caacggaaag acccgtgcgg
     1561 gcgttgagcg aataaaccac ctgcgaagcc agcataaacg ggtagccgta actgggtggc
     1621 agaaaggcgg ttaatgtgac gctggtggcg cttaattcgc tgatttgcca gtcacgccag
     1681 gcgagcagcc catgaatagc ggcttttgaa ccatgttcat taatgggtaa ctgatattcc
     1741 tgcccctgat agcgataaca gccgttggcg atccggtttg gccacggaat aaggactttg
     1801 cccagatgtg ccagcggcat ctcttcgggt ttatgcggaa taactaaatg acagccctga
     1861 aaggttaatt cagccagacc tgcaccgacg gtcacaatgg tcgcgtgata atcccccgcg
     1921 gcaagtgaaa cggtctgtcc actgcaatgc atgttggtga tttgcatacg aggtaccttt
     1981 attgaataac gacatacccg gcgtgcagcc tgatatgtcg tttttttagt cagttatttc
     2041 gcgttaatat ccagcagacc cgccgcaacc gccggagcca ggcctggcgc taacgggata
     2101 cgtgggatca ccagacaatg cagcagatga taaatatcct gtttgccgtc ccagactttg
     2161 gtggtgacct tattgtccgc atccagctcc tgccaccatg aaccattttc gtagtccatc
     2221 aggtatttga tgcagtagtc ccaccatgtc tgataccaag tttcgtactg gcgatcgcca
     2281 gtgacggtgt agagcgcgta ggccgtaccc atcgcttcga cgataggcca acgcacacgt
     2341 tcgcggacca ccggttttcc ttcccagtca acggtataaa caatcccgtc cgcgccatcg
     2401 ggtgcccagg catcgcgcac ggtggcgtta aacagacctt tggcatcttc tagcagccat
     2461 gctggcggtt gttcgcaacg ggcttccagg gccgcgtgaa tgtgcagcat taaacggccc
     2521 cattcgatcc agtggcccgg agtacctccg aacgcgcgga agcggtgcgc cgggttatct
     2581 ttgttgtaat ctggcagcgg attccactgg gtatcgaagt gttcgttaac gcgataatga
     2641 ttatttctgg cgacgtcgtg gataatcacg gaagccacgc gaatcgcgcg atccagccat
     2701 tttttgtcat gggtgacgtc atagacaatc aggaaggctt ccaccgcgtg catattggca
     2761 ttgccgccgc gatactcttc ggttttgctg aaggcttcat cccaggactc caggcacatc
     2821 tgctcttctt cgctccagaa gtatttctcg ataatttcaa tggtgtaatc gagcagcttg
     2881 cgcgcttccg gatgaccggt agtgacggcg ctggcagcac ccagcagagc aaagaaatgt
     2941 tgatagccct gtttggaggc atccaccacg ccctcgtcat tcacgcaggc ataccaaccg
     3001 ccatattttt tatcgcgcag tgcgccattc atggctttga taccgtgatc aaccaacgag
     3061 taagcgccag gtcggcccat cgccgcagca acggaataaa cgtgcaacat acgagctgtg
     3121 atccacagat gggtgcccat ctcttctttg atttgccctt tattgcctaa ccagccaaat
     3181 ccggtcggta ctacggattt tttaccaaaa tcaaagatgc gatcggtttc ctgttccagc
     3241 caacggttgt gacttaaagt gttaaaccat ttcatttttg tatccccatc ttaacggcgt
     3301 ttagccatca tttcgtcgac gatatcgcca agttgttgta atttcggtgc gcaaacgtca
     3361 cgcagcatca gctcgttgtc tggtaaaccg acgaccgatg cccagacggc acgaccagcc
     3421 aggaagcccg atgcacccgc tgtcattgcc acgcgtacgg cacgcgggaa cagtttttcg
     3481 tcgacaccgg aagagaggat cacccacggc atattgatat ggtcattcag acgttgtgaa
     3541 gcactgagaa gctcttgttg cggaccttta ccgtaaagcg gcatttcaac tttgtagaga
     3601 tcagcgccac tgtcgcccag ctctttggct gcatcgatga ttgcttgttc gcgatcgaat
     3661 ttatcgccac gacgcggtgg gcggacgact ggctcaatga tgcttaccag accgtgtgag
     3721 tggcacattt cgttaaactc tttcaccata tccaggcgtt gctgcgcatc ttcatcacta
     3781 cgccacagca ccagcagttt taaggctttg ccaccgtctt gtttgatttg tagcggattg
     3841 attttgcggt caatgaccac gctatctacc ggaataccgt tgccaggaat gaactcatcg
     3901 gcggcgacaa tcatggcgca acttttggca atcgcgtttt gctcaaccac ctggcggtag
     3961 cagaattgtt gatctaccag aatcgccgat gcataaggtg aaagagtctt cgcggcgtta
     4021 actttgaaat cagttaaaac gctatcggct actggagcgg gtgcgccagc cgcggcaaac
     4081 atcatgcgca tggcttcgcg ctgatcgacc gccagcatgg caaaaccgcc cgatgcgcgc
     4141 gtaatgtcgt tgatggtgta cttattcatt catttattcc tttatccagt cgttacattt
     4201 ttactttggc agtcatcccg gcactgacac ggacctgctc cagaatggcg gaccagtcct
     4261 ggcgaccgcg acccgctgcg cgcgcctggc tataaacctc ccgtgaggcg gcccccagtg
     4321 gcatcggcac atgcaactgg ttggcgacat caagggcgat gccaagatcc ttatgggcaa
     4381 gatcgatcat gaaggcggga gaaagatccc cgctgaggac tttgttcggc caggaagtgg
     4441 tgaagtggcc tttaccggcg gcggtaccgc tcatcacctt gacggcaaca tcgaagggaa
     4501 gattcagggc ttcgcacaaa acggcggctt ctgccgacaa cgcattgagc gcgatgctca
     4561 tgtagttgtt gataagctta acgcggatcc ccatgcccgg accgcccgcg ttgatcaact
     4621 cgctgcccat cgccatcagg atcggcgtgg cgcgttcaac ttgttcagca gtgccgccag
     4681 ccagcagtag cagagtaccg gtaatggcat ttgcagaagt acggcctacc ggaacatcca
     4741 tcatgctgaa gcctctggcc tgcatatcag caatcaattt atcggtttgc agcggatgga
     4801 tggtcgacat atcaatgacc agcgcatcgg aagataagct ctcacagaca ccgttttcac
     4861 cgaacaacac gctgcgcacc agatcaccat tcggcaacat ggtaatgata aattcggcat
     4921 ctttagcggc ctgcgccggg ttggcggcgg gagtcgcacc tttgtctacc agatgccgca
     4981 cagcttcggc gttcacatca aagacgcgaa gttgatgccc ttgctgcaat aaattgctcg
     5041 ccattggcga acccatttgc cctaaaccga taaacgcgat tgctgccata accctctcct
     5101 gaatacagta atgtcacttt ttgtcattta tgacatgcct tgcttgtctg tttttgatcg
     5161 tatttgtaat ttatcgtcaa aaaattgaca gccgtcactt tttaaacaat tggtgaaatt
     5221 aaaatgaacg catcccaaaa tgcttaagga atgaccatga ttcgtgttgc ttgtgtagga
     5281 attaccgtga tggatcgcat ctattacgtg gaaggattgc cgacggagag cggtaaatac
     5341 gtggcgaaaa attatacgga agttggcggc gggccagcgg ctacagcagc agttgcggcg
     5401 gccagactag gtgcgcaggt cgattttatt ggtcgcgtcg gtgacgacga cacaggcaac
     5461 agcctgctgg cagagctgga atcctggggc gttaacaccc gttataccaa acggtataac
     5521 gaggcgaaat cttcgcaatc cgccatcatg gtggatacca aaggcgagcg gataatcatt
     5581 aactacccca gcccggactt gctgcctgac gcagagtggt tggaggaaat tgatttctct
     5641 cagtgggatg ttgtgctggc ggatgtacgc tggcacgaag gtgctaaaaa agccttcacc
     5701 ctggcccggc aggcgggcgt gatgactgtt ttggacgggg acattacgcc gcaggatatc
     5761 agtgagctgg tggcattaag cgatcatgcg gccttttcag aaccgggtct ggcgcgctta
     5821 acgggggtga aagagatggc cagtgcgcta aaacaggcac aaacgctcac aaatggtcat
     5881 gtctatgtga cccagggcag cgcaggctgc gactggctgg aaaatggcgg gcgtcaacat
     5941 caaccggcct tcaaagttga tgtggtagat accacaggag cgggtgatgt ttttcacggc
     6001 gctttggcgg tggcgctggc cacaggtggg gatttagcgg agtcagtccg cttcgccagc
     6061 ggtgtagcgg cgttaaaatg cacacgtccc ggtggacgag ctgggatccc tgactgtgat
     6121 caaacccgat cttttttgtc actttttgta taaaatgcca gggtgatggt ttttcgagga
     6181 attttcatga gccttaccga actaaccggt aacccgcggc acgaccaact cctcatgctg
     6241 atcgccgagc gtgggtatat gaatattgat gagctggcaa atctgctgga tgtctccacg
     6301 cagacggtcc gccgggatat tcgtaaatta agcgagcaag gactgattac gcgccatcac
     6361 ggtggcgcgg gtcgggcttc cagcgtcgtt aatacggcgt tcgagcagcg tgaggtttcg
     6421 caaaccgaag aaaaaaaagc gattgccgaa gcggtggcag attatattcc tgatggttca
     6481 acaatattta tcaccattgg tacgaccgtt gagcatgttg cccgggcgtt acttaaccat
     6541 aaccatttgc ggataatcac caacagcctg cgtgtggcgc atattcttta ccacaacccg
     6601 cgctttgaag tgatggtgcc tggcggaacg ctgcgttccc ataacagtgg gatcattggc
     6661 ccttcagcgg cgtcctttgt ggccgatttt cgcgccgatt atctggtaac aagtgtaggc
     6721 gcaattgaga gcgatggcgc gttgatggag tttgatgtta acgaagctaa cgtggtgaaa
     6781 acgatgatgg cgcacgcgag aaatattctg ctggtcgccg atcacactaa gtatcatgct
     6841 tcggcggcgg ttgaaattgg taatgtggca caggtcactg cgctctttac cgacgagctg
     6901 ccgcccgctg cgctaaaatc acgcttacaa gacagccaaa ttgaaatcat ccttccccag
     6961 gaagacgcgt agaattttgt gaccgttgac ctggcttcat acctgaccac atagccaaac
     7021 ccatcctttc ccgctacagt taatttcttg tggcgcgaaa ggaggcaaaa atgctctata
     7081 tctttgattt aggtaatgtg attgtcgata tcgactttaa ccgtgtgctg ggagcctgga
     7141 gcgatttaac gcgtgttcca ctggctacgc ttaagaagag tttccacatg ggcgaggcgt
     7201 ttcatcagca tgagcgtggg gaaattagcg acgaagcgtt cgcagaggcg ctgtgtcatg
     7261 agatggctct accgctaagc tacgagcagt tctctcacgg ctggcaggcg gtgtttgtcg
     7321 cgctgcgccc ggaagtgatc gccatcatgc ataaactgcg tgagcagggg catcgcgtgg
     7381 tggtgctttc caatactaac cgcctgcata ctaccttctg gccggaggaa tacccggaaa
     7441 tccgtgatgc tgctgaccat atctatctgt cgcaagactt gggtatgcgc aaacctgaag
     7501 cacgaattta tcagcatgtt ttgcaggcag aaggtttttc accagacgat acggtctttt
     7561 tcgacgataa cgccgataat atagaaggag ctaatcagct aggcattact agtattctgg
     7621 tgaaagataa aaccaccatc ccggactatt tcgcgaaggt gttatgctaa aaaccattca
     7681 ggacaaagcc aggcatcgta cccgtccact atgggcctgg ctaaaactac tctggcaacg
     7741 cattgatgag gacaacatga caaccctggc aggtaacctt gcctatgtgt cgttgctctc
     7801 attagtgccg ctggttgccg ttgtttttgc gcttttcgcc gcttttccca tgttttccga
     7861 cgtcagcatt cagttgcgtc actttatttt tgccaacttt ctgcctgcta ctggcgatgt
     7921 tatccagcga tatatcgaac agtttgtcgc caattccaac aaaatgaccg ccgtcggggc
     7981 gtgcgggctg atcgtcacgg cgttattgtt gatgtactcc atcgatagcg cgttgaatac
     8041 catctggcgc agtaaacgag cgcgacccaa aatttactcg tttgccgtgt actggatgat
     8101 tttaacgctg gggccgctgt tggcaggggc cagtctggcg atcagctcct atctgctctc
     8161 tctgcgctgg gcgagcgatc tcaatactgt catcgataat gtgttgcgta tttttccgct
     8221 gctgttgtcg tggatctctt tctggctgct gtacagcatc gttcctacca tccgtgtacc
     8281 taaccgcgac gcgattgtcg gcgcgtttgt cgccgcgctc ctgttcgaag caggaaagaa
     8341 aggtttcgcg ctttatatca ccatgttccc atcatatcag ctcatttacg gtgtgctggc
     8401 ggtgatcccc attctctttg tttgggtcta ctggacatgg tgtatcgtct tgcttggcgc
     8461 ggaaattact gtcactctcg gggaataccg caaattaaaa caagctgctg aacaagaaga
     8521 agacgacgaa ccatgattgc attaattcaa cgcgtaaccc gtgccagcgt caccgtggag
     8581 ggagaagtga cgggcgaaat tggcgcggga cttttggtgt tattgggtgt cgaaaaggat
     8641 gacgacgaac agaaagcaaa ccgtctgtgc gagcgtgtgc ttggctatcg catcttcagc
     8701 gatgccgatg gcaagatgaa tctcaacgtg caacaggcgg gcggcagtgt gctggtggtt
     8761 tcccagttta ccctcgccgc agataccgaa cgggggatgc gcccaagctt ctcaaaaggc
     8821 gcatcaccgg atcgcgcaga ggcgttatat gactatttcg tcgaacgctg ccgtcagcag
     8881 gagatgaaca cgcaaacagg gcgcttcgct gcggatatgc aggtatcgct ggtcaatgat
     8941 ggccccgtga cattctggtt gcaggtatga gccagcttcc aggtgtgtca cgggaaacaa
     9001 gagagagtat cgctatgtat caccttcggg ttccacaaac agaagaagaa ttagagcgct
     9061 actatcagtt ccgctgggag atgttgcgta aacccctgca tcaaccgaaa ggttcggaac
     9121 gcgacgcgtg ggatgcgatg gcgcatcacc agatggtcgt cgacgagcag ggtaatctgg
     9181 tggcagtagg gcggctgtat attaatgccg ataacgaagc gtccatccgt tttatggccg
     9241 ttcatcccga cgtgcaggac aaagggttag gcacgctgat ggcgatgact ctggagtcgg
     9301 tggcgcgtca ggaaggcgtt aagcgcgtga cctgtagcgc ccgtgaagac gcggtggagt
     9361 ttttcgccaa gctggggttt gttaatcagg gagaaatcac cacgccaacc accacgccga
     9421 ttcgccattt tttgatgatt aaacctgtcg ccactctgga tgatattttg catcgcggcg
     9481 actggtgcgc gcagctgcaa caggcgtggt acgaacatat cccgctaagc gaaaaaatgg
     9541 gcgtgcgcat tcagcaatat accgggcaaa aatttatcac caccatgccg gaaacaggca
     9601 atcagaatcc gcatcacacg ctgtttgccg ggagtttatt ctcgctggca acgctcactg
     9661 gttgggggct tatctggctg atgctgcgcg aacgccacct cggcggaacg attattctgg
     9721 cggatgcgca tatccgctat agcaaaccga ttagcggaaa accccatgcg gtagccgacc
     9781 ttggtgcctt aagtggcgat ctcgaccgtc tggcgcgcgg acgaaaagca cgggtgcaga
     9841 tgcaagtaga aatctttggc gacgagacgc cgggtgcagt gtttgaaggc acgtatatcg
     9901 ttctgcccgc gaagccattt ggtccgtatg aagagggcgg aaacgaagaa gagtaggggc
     9961 gttttacttg actgaaaagg ttttcaatct gaaaaccttt tctgggccct ggtccggagt
    10021 taccttcccg ctctccaggc aacatcccgt agcgccgatc gcgcagcctc actgatacca
    10081 cctaactgaa aaaatccgtg gataacgcca aggtagcgtt ggcaggtgca ttgcactcct
    10141 tgccctgtca tgcggtgata caacgcttca ccttcatcgc aaagtgggtc gtattcagcg
    10201 gtaatgatat gtacaggtgg caagccattg aaatcattgc gccaaatagg gctggcttca
    10261 ggatgcctgc gttcaatgcc tggcaggtac atctcaaagc cgctcagtaa agtgtcgcgt
    10321 gtgatgatgt aatcgtgacc attgcgtatg tagctttcga aatgcgcagt cgcatcaagc
    10381 ataggataaa ttaagatgag ttgtgctggt tgccactcgc cagcacgctt caatcgcaaa
    10441 gcagttacca gcgccagatg ccctcccgca ctgtcaccgg caagagttat tcgttgttta
    10501 tcaacgccga gtcgttcggc gtgttgccgg actaattctg cacctcgttg tgcatcatca
    10561 tgggcggcgg ggaaagtatg ttccggtgcc agccgatact ggacggcaat aatccgacat
    10621 tgcccgtaat aggctaactg gcgcagctgg ttgtcgtgag ttgcgaaccc gccgctaaca
    10681 aaacagccac cgtagtaata aattatcgtc gggagtaatg tcggggcatt cagcggtgac
    10741 actattcgta aggtcatccc ttcaagttcg atagtctgaa tatctacccg cgtttctgtc
    10801 tcacccgcaa ggacagcgct ggcgatatag ccctctctgc gctgcgtaat actttgttgg
    10861 cgcgatgagg ggcgacccgc agcgataaac tcttcgacta actctgcaat tcctttttcc
    10921 agtgccatag tagaactcat tttgctgtat gaatgtacag ttttatacac ccgtttttgc
    10981 tggatggata cagggaaatt cacgggcgtg aaaaatctgg aaagcacctc gcaaatgtaa
    11041 agacaaaccc ggcagagacg tggttttgct ggagcactat cagtaagcta gtggtttcaa
    11101 tggtacgtca atgacgtaca aaaaggattg ttactctaag ccaatggtgt ataattatgc
    11161 tcttcatcga aacggaaatt tttactgaag atgttcaaaa actgctgaac gacgatgaat
    11221 tcagtcgttt tcagtttttc ctggccttga atcctgacta tggtgaggta atacctgaaa
    11281 ctggtgggtt aagaaaggtt cgatgggtct caggaggaaa ggggaaacgt gctggtgtca
    11341 gggttattta ctttcatcag gttaaacatt atgagatcag attactgctt atttatcgta
    11401 agggtattaa agatgatctg tctccccagg aaaaagcaat gctccggttg ttaaatacga
    11461 ggtggtaaat ggataaggca ctttttgagc ggttgactca cagcatggcc cagatgaatg
    11521 aaattattga aggaacgcga cagccttcac gtacttttga agttgatgct atgaaaatca
    11581 aagaaatacg ccgcgcttca gggttgtcac aatctaaatt tgccgatctt atttccgtaa
    11641 gcgtagacac gttgcgtaac tgggagcagg ggcgacgttc accgaccggg cctgcaaagg
    11701 ctttgctgcg agcgattgcc aatgatccgc aacatgtgct gcaagcactt aaccgttaga
    11761 ggcatggtta tgccatacag aaagcaccag cattcagaac tcctatgaaa gcggcatagt
    11821 gccgcctgct caggagtttt gctatgaccc gtaatcgtgc tgaacgtcgc catcatatgg
    11881 tgcgattaaa aaaaatccgc tgtcgttatc gcactgcagg cgatggaagc aaaaaagcat
    11941 caggtatttg ctttcgtact ccctgtattt gttcttgttg gatgtgtggg catcgacgct
    12001 atcataatgg tccccgcgta tcggaaattc gtgcaaaagc acgctatgcg aattaaggga
    12061 aaatgatgaa ctattttaag gaagcacatt tttagaactc ttaacaggag actaaaaatg
    12121 tccacgttta atacgcgtaa agatagggat cgtaatggta aagcgaagcc gcataagcat
    12181 tcagggatcc caacacttac aggtaaatgg cgtcaccctt caatggattg ttgggtaaac
    12241 tacagtacac cgggcgatca tgaatggaag cggctctata ctacgcttcg ccgtcgcgca
    12301 gatgatcgac gccaatgtca tcgggtaatg cagggggatg atccggaagg aatcgtctgg
    12361 ttcccggact gttatccaca aatttactac tactaacaat taaagaccgg ttggtgcggc
    12421 actgaccggt cgttatgaat ggtagatttc accttaaacc ctggtgcggt aaagctgctg
    12481 actttttcgg gtacaccatc ctgatgaagc aaatgaagac ccaatcaaaa aatattcttg
    12541 aa
//
LOCUS       DAZFRG010000141        12444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFRG010000141 DAZFRG010000000
VERSION     DAZFRG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.141"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..402)
                     /gene="ynfD"
                     /locus_tag="ACHKUC_003177"
     CDS             complement(97..402)
                     /gene="ynfD"
                     /locus_tag="ACHKUC_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO6619391.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
     gene            510..1220
                     /gene="ynfC"
                     /locus_tag="ACHKUC_003178"
     CDS             510..1220
                     /gene="ynfC"
                     /locus_tag="ACHKUC_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO6619392.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(1223..1783)
                     /gene="speG"
                     /locus_tag="ACHKUC_003179"
     CDS             complement(1223..1783)
                     /gene="speG"
                     /locus_tag="ACHKUC_003179"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO6619393.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(1818..2159)
                     /gene="ynfB"
                     /locus_tag="ACHKUC_003180"
     CDS             complement(1818..2159)
                     /gene="ynfB"
                     /locus_tag="ACHKUC_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO6619394.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            2294..2620
                     /gene="ynfA"
                     /locus_tag="ACHKUC_003181"
     CDS             2294..2620
                     /gene="ynfA"
                     /locus_tag="ACHKUC_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO6619395.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2826..4040
                     /gene="rspA"
                     /locus_tag="ACHKUC_003182"
     CDS             2826..4040
                     /gene="rspA"
                     /locus_tag="ACHKUC_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO6619396.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            4052..5071
                     /gene="rspB"
                     /locus_tag="ACHKUC_003183"
     CDS             4052..5071
                     /gene="rspB"
                     /locus_tag="ACHKUC_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO6619397.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            5129..6496
                     /gene="ydfJ"
                     /locus_tag="ACHKUC_003184"
     CDS             5129..6496
                     /gene="ydfJ"
                     /locus_tag="ACHKUC_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO6619398.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQ
                     PSRSL"
     gene            6585..8045
                     /gene="ydfI"
                     /locus_tag="ACHKUC_003185"
     CDS             6585..8045
                     /gene="ydfI"
                     /locus_tag="ACHKUC_003185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6619399.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     MIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRKWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(8081..8284)
                     /gene="ydfZ"
                     /locus_tag="ACHKUC_003186"
     CDS             complement(8081..8284)
                     /gene="ydfZ"
                     /locus_tag="ACHKUC_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO6619400.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(8462..9148)
                     /gene="rspR"
                     /locus_tag="ACHKUC_003187"
                     /gene_synonym="ydfH"
     CDS             complement(8462..9148)
                     /gene="rspR"
                     /locus_tag="ACHKUC_003187"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO6619401.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATIDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(9237..9983)
                     /gene="ydfG"
                     /locus_tag="ACHKUC_003188"
     CDS             complement(9237..9983)
                     /gene="ydfG"
                     /locus_tag="ACHKUC_003188"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO6619402.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            10120..12165
                     /gene="dcp"
                     /locus_tag="ACHKUC_003189"
     CDS             10120..12165
                     /gene="dcp"
                     /locus_tag="ACHKUC_003189"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO6619403.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDVAQLAGMSEQEIAQAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWMRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSRAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(12210..12290)
                     /locus_tag="ACHKUC_003190"
                     /pseudo
     CDS             complement(12210..12290)
                     /locus_tag="ACHKUC_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12314..12418
                     /locus_tag="ACHKUC_003191"
                     /pseudo
     CDS             12314..12418
                     /locus_tag="ACHKUC_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
BASE COUNT         3148 a         2816 c         3168 g         3312 t
ORIGIN      
        1 atccatatca atagaagggt atgagtaata aggtgggatt atgttgtatg ttcaaatcgc
       61 cggatgtgtc gtatccggcg ttcagtcgat aatgtgttac tgcggttcag caggcgcgtt
      121 atcctgggta gactgcgcgg gagcagagac gttaccgctg gtggtacggg tgtagagaat
      181 tttatgcgta tcattagcgc aatgcccgac gacctgggaa tcaggctgat caacctggtc
      241 attgggtaca atacttaacg tgaagctgct ttcgggtacg ccattattga taatgcgctg
      301 tgatatatcg ctctgtatgc gctcacagga tcccggcgcg gcgagtaccg cgggtgaggc
      361 gagggcgagc agaagcgcgg cacagcaggt tgagagtttc atcataagct ccttacgcga
      421 agataacttc tttaagcata gcatttaacg tgtaaagtac tgtatttgct actatgattg
      481 agaatcatct ctactctcag gtgactgttg tgaaatacaa attactgcca tgcttactcg
      541 cgatattact cacaggatgt gaccgcacag aggtaacact ttcatttacc ccggagatgg
      601 ccagtttctc taatgaattc gattttgatc cgctgcgtgg tcccgtaaaa gatttcactc
      661 agacattaat ggatgagcaa ggtgaagtga cgaaacgtgt ttcggggact ttgtcggaag
      721 aaggctgttt tgattctctc gaattactgg atctggaaaa taataccgtg gttgccctgg
      781 tactggacgc caattattac cgtgatgccg agacgctgga gaaaagagtt cgtttgcagg
      841 gaaaatgcca gctagcagaa ttaccttctg ccggggtgag ttgggaaacc gatgataatg
      901 gtttcgtgat taaagccagc agcaaacaga tgcagatgga atatcgctat gatgatcagg
      961 ggtatccgct gggtaaaacc acgaaaagta acgataaaac attatctgtc agcgccacgc
     1021 catcaacgga cccgatcaaa aaattagatt acacagcggt tactttactg aataatcaac
     1081 gggttggaaa tgtaaaacag agctgtgaat atgacaatca cgccaatccg gtggactgtc
     1141 agctaatcat tgttgatgaa ggagtaaaac ccgccgtcga acgggtttac accatcaaaa
     1201 atacgatcga ttattattaa tactattgtg cggtcggctt caggagagtc tgacccggtg
     1261 ttttgtgctc agccagatac tgatgctgga atatacacat gcgaatggca ttacgatatt
     1321 gaccattaat aaagaactca tgcatcaatt caccttcaac cgtaaagcca agcttgcggt
     1381 aaatgtgaat cgctttttca ttctctttat caacgatcag atacagctta tagagattga
     1441 gaacggtgaa gccatagtcc attgctaatt tggcggcacg ggttgccaga cctttcccct
     1501 gatactccgg ggagataatt atctgaaatt ctgcgcggcg atgaacatgg ttaatttcca
     1561 ccagctccac cagaccggct ttttcgccgt cacattccac cacaaagcgc cgttcgctct
     1621 gatcgtgaat atgcttatca tacagatcag agagttcaac aaaggcttcg tagggttcct
     1681 caaaccagta acgcatcaca ctggcgttat tgtcgagttg atgtacatag cgtaaatctt
     1741 cacgctccag cgggcgtagc ttaacactgt gggcgcttgg cataacgtgt ccttacattc
     1801 cttaaatcaa taacaggtta ggggataata acgcggccag ttcgacggtc caggcagcgc
     1861 aaagtatttg gctcccagta ggcattgatg ttggcgcttt gctcacattt atcgcggtta
     1921 tcaaaagcag cgtcggcttt atcccactct ttttcagtgc gtttattcac tttctggcgc
     1981 agattacgcg tgtcattcca ttgctctttt tccatagcgg cgcgctggcg gctttgtgca
     2041 ctgtcgccag actcaatcac cagtttgtta gtttcggcat gaacagttgt gctcaatgcc
     2101 agtgcgcaag gcagcagaaa agcgagcagg ccgattcgtt tgctgagagt gattttcata
     2161 attcattccc tgtatgaatg attaaagctg attctacacc atccactgcg aacgcaaaac
     2221 gttcaaggag ggtgtttata ttgatgatat tatgtcgccc tataactata cgtgatgtca
     2281 ataagtgaca aagatgatta aaacaacgtt actatttttt gctactgcgc tgtgtgaaat
     2341 tattggatgc tttttgccct ggctgtggtt gaagcgaaac gccagtatct ggctgttgct
     2401 tccggcgggg atttcactgg cgctgtttgt ctggttgtta acgttgcatc ctgcggcgag
     2461 tgggcgtgtt tacgcggctt atggtggcgt ttatgtctgc acggctttga tgtggctgcg
     2521 cgttgtggat ggcgtgaaac tgactcttta tgactggacg ggggcgttga ttgcgctttg
     2581 cggcatgttg atcattgttg cgggctgggg gcgcacgtag gaacataaat ccattttatc
     2641 aataagatac gaggaagtgt cagctgacaa aaggtattct gtttcatctt ttgtcaacca
     2701 ttcacggcgc aaatatacgc ctttttttgt gatcactccg gctttttttg atcttcatac
     2761 ttgtatggta gtagctcagt tgcgtagatt tcatgcatca cgacaagcga tgcaaggaat
     2821 cgaacatgaa gatcgtaaag gctgaagttt ttgttacctg tccggggcgt aatttcgtca
     2881 cattaaaaat caccactgag gacggtatta cgggccttgg ggatgccacc ctaaatggac
     2941 gtgagctttc cgtggcctct tatttgcagg atcacctttg tccgcagctt attggtcgcg
     3001 atgcacaccg tatcgaagat atctggcagt ttttctataa aggtgcttac tggcgtcgcg
     3061 gtccggttac gatgtcggcc atttcagcgg ttgatatggc gctgtgggat attaaagcca
     3121 aagctgccaa catgccgctt taccagttac tcggcggcgc gtctcgtgaa ggggtgatgg
     3181 tttattgcca taccaccggt cacagtattg atgaagctct ggatgattat gcccgtcatc
     3241 aggagctggg attcaaagcc atccgcgtgc agtgcggaat ccctggtatg aaaaccacct
     3301 acggcatgtc gaaaggtaaa ggtctggctt atgaacccgc aaccaaagga cagtggccgg
     3361 aagagcagct gtggtcgacg gagaaatacc tcgatttcat gccgaaattg tttgacgcgg
     3421 tacgtaacaa gtttggtttt gatgaacatc tgctgcatga catgcaccat cgcttaacgc
     3481 ctattgaagc ggcgcgcttt ggtaagagca ttgaagatta tcgcatgttc tggatggaag
     3541 acccgacgcc tgcggaaaac caggaatgct tccgtctcat tcgccaacat accgtcacac
     3601 ccatcgcggt gggtgaagtc ttcaacagca tttgggactg caaacaactg attgaagagc
     3661 aactcatcga ttatatccgc accacgctga cccatgcagg cggaattacc ggtatgcgtc
     3721 ggattgccga ttttgcttcg ctgtatcagg tacgtactgg ctcacacggt ccttccgatt
     3781 tgtcaccagt ctgcatggct gcggcgctgc actttgatct gtgggtcccc aatttcggtg
     3841 tccaggaata catgggttat tccgaacaaa tgctcgaagt cttcccgcac aactggactt
     3901 tcgataacgg ctatatgcat ccgggagaaa aaccgggtct tggcatcgaa ttcgatgaaa
     3961 agctggcggc gaaatatccc tatgaacctg cttatctgcc agtcgcacgt ctggaagatg
     4021 gcacgctgtg gaactggtaa ggagtaaggt aatgaaaagc attttaattg aaaaaccgaa
     4081 tcaactgtcg attatcgaac gtgaaatacc caccccgtca gcgggtgaag tacgagtaaa
     4141 agttaaactt gccggaattt gtggttcaga tagccatatt taccgtgggc ataatccttt
     4201 tgcgaaatat ccgcgcgtca ttggtcatga attctttggc gtcattgatg cagtgggtga
     4261 cggcgtggaa agcgccagag tcggtgaacg cgttgctgtc gatccggtgg tcagctgtgg
     4321 gcattgctat ccgtgctcta taggtaagcc gaacgtttgt acgacacttg ctgtattagg
     4381 tgtgcacgct gacggtggtt tcagtgaata tgccgtggtt ccggcaaaaa atgcgtggaa
     4441 aattcctgaa gcagtggccg atcaatatgc ggtgatgatc gaacctttta ccattgcggc
     4501 taacgttacc ggtcatggtc aaccgactga aaatgatacc gttctggttt acggtgccgg
     4561 cccaatcggc ctgacgatcg ttcaggtatt aaaaggcgtc tataacgtta aaaatgtgat
     4621 tgttgccgat cgcattgatg aacgactgga aaaagcgaaa gagagcgggg cagactgggc
     4681 gattaataac agccagacac cgcttggcga gagtttcgct gaaaaaggta tcaagccgac
     4741 tttaattatc gatgccgctt gtcatccttc aatcttgaaa gaggccgtaa cgctggcttc
     4801 tccagcggca cgtattgtat tgatgggctt ctccagtgaa ccgtctgaag tgattcagca
     4861 aggaattacc ggaaaagaac tctctatttt ctcttcacgc ttaaatgcaa ataaattccc
     4921 ggtcgttatc gactggttaa gtaaagggtt aattaaacca gaaaaactaa ttacccatac
     4981 atttgatttc cagcatgttg cggatgccat tagtttattt gaacaggatc aaaaacattg
     5041 ctgcaaagtc ttactcactt tttctgaata ataccaataa cggcgagtaa gtagtacgca
     5101 tcttacctct tttttagaga taaccattat gacaatagaa aaacacgaaa gaagtactaa
     5161 ggatttggtg aaagcagcag tatcgggatg gttgggcact gcgcttgaat ttatggactt
     5221 ccagttatat tcgctcggcg cagcgttagt gtttcatgaa atattttttc ctgaatcatc
     5281 aacggcaatg gcgttaattc tggcaatggg aacctatggt gcaggttatg tggcacgtat
     5341 tgtcggagca tttattttcg gcaaaatggg cgacagaata gggcgtaaaa aagtgctctt
     5401 tattaccatc accatgatgg ggatctgtac caccttaatt ggtgtgttac cgacctatgc
     5461 acagattggt gtttttgcac ccatcttgct ggtgacgctt cgtattattc aggggttggg
     5521 tgcaggtgcg gaaatttccg gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa
     5581 gcgcggaatt atctcctcat ttgtggctat gggaactaac tgcggaacct tgagcgcaac
     5641 agcaatctgg gcctttatgt tcttcattct cagtaaagag gaactgctgg cgtggggatg
     5701 gcgtatacct ttcctggcga gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa
     5761 tctgaaagaa agcccggtct ttgagaaggt taacgacagc aaccaaccga cagcaaaacc
     5821 tgcacctgct ggtagcatgt tccagagcaa atccttctgg ctggcaacag ggctgcgttt
     5881 tggtcaggct ggtaactcag gtttaattca gactttcctt gcaggctatt tagtgcagac
     5941 gttattgttt aacaaagcaa ttccaacaga tgcattgatg atcagttcga ttctcggctt
     6001 tatgaccatt ccgttccttg gttggttatc cgataaaatt ggtcgccgga tcccgtatat
     6061 tattatgaat acctctgcga ttgtgctggc atggccaatg ctttctatca tcgtagataa
     6121 aagctatgcc ccgagcacca ttatggttgc actgattgtg attcataact gtgcggtgct
     6181 tggattattt gctctggaaa acattaccat ggcagaaatg ttcggctgta aaaaccgctt
     6241 tacccgaatg gctatttcta aagaaattgg tggtcttatc gcttccggtt ttggtcctat
     6301 cctggcgggg attttctgca ccatgacgga atcctggtat ccgatcgcca ttatgatcat
     6361 ggcatattct gtgattggtt taatctctgc gctgaaaatg ccagaagtga aagaccgtga
     6421 tttaagtgcg ctggaagacg ccgcagaaga tcaaccacat gttgtaagag ctgcgcaacc
     6481 gtccagaagt ctgtaaatcc tcaatccctt ctcttaccgg agaggggatt tttattcata
     6541 taacaaaaca tgtagcttgc tatatttaat tttaaggaat tgttatggga aatcatttgt
     6601 tatcagcaaa agcgacgctc cctgtttatg atcgtaataa cctggcccca agaattgttc
     6661 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
     6721 cggaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
     6781 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gctgatgcgt
     6841 ggacggctcg cgtcgttggc gtcgttaaaa aagccttaca cgtacaaata gatggcttag
     6901 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
     6961 aaaaagggta tttccactct ccggcgaccg gacagttaat gcttgatcac ccgatggtcg
     7021 ttgccgacgt gcaaaatccc caccaaccga aaactgcaac agggatgatt gttgaggcgc
     7081 tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
     7141 cagaaaacgg tcatgttatg cgtgacgttg tcacttccta tgcgcaagcc attgatgtaa
     7201 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
     7261 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgcgcgatc
     7321 cggcaggcgt tgcctgtgaa cctttccgca agtgggtaat tgaagataac tttgttgccg
     7381 gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
     7441 agatgaagtt gcgcatgctc aatggcagtc attcattcct ggcgtatctg gggtatcttg
     7501 ccggatatca gcacattaat gactgcatgg aagatgaaca ttatcgtcat gcagcgtatg
     7561 ccttaatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     7621 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     7681 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     7741 tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg
     7801 tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgatccactg ttacctgtta
     7861 ttcagaaggc agtacaaagt agtgctgaag ggacagcgcg cgtccagtca ttgctggcaa
     7921 ttaaggcgat ctttggtgat gatttaccag gcaatagttt gtttacgaca aaagtaacgg
     7981 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     8041 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     8101 tcaggtccag tggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     8161 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     8221 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     8281 tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     8341 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgatgca
     8401 tgcatgagcc ataagtcggg gggaaaagac tctcaccccg gatgggatga gaggaaatga
     8461 attactcttc gctaaaccag tcgctgtttt cctggcggat ctggcgcacg gattcgctga
     8521 tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt ttttgcaggg
     8581 cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaacg tggtcgaaac
     8641 tcatatagcg cacgcgatca atggtcgctt tcaggttctc aatggtatcc cacgccagtt
     8701 gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca aaaaaatcat
     8761 ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc agttggtagc
     8821 actggctttc ggtaatcatg ctcgccgccc gacgcgccac cgcgcactca atggcctgac
     8881 ggataaaact gccgttgcgc acctgggcca tggaaatttt gttgacgtag ctgccacgct
     8941 gcggacgaat ttgaatcaga ccgttttccg ccagtttaat aaaggcttca cgaaccggct
     9001 ggcgtgacac attgaaacga acagaaactt ctttttccga caacggtgtg cctggtgcaa
     9061 tcaggcaatg gacaatgtcg cggcgaagaa tacgataaat ctgctgattg acaggctgtg
     9121 tgggattaag ttgcgtttcg acggtcattc gttcttgctt gtgagtgagt taactgcatg
     9181 agtctaccac ttttttgtga caagcaataa cccggcagtt acgccgggta taaaaattac
     9241 tgacggtgga cattcagtcc ggcatagctt tgggtaaccg gcatcatttc cagggtattg
     9301 atattgacgt gagcaggcag cgttgacacc caccagacgg cttcgctgac atcttctggt
     9361 gtcaatgcaa cggtattttg ataggttttt tccgctttac cgtcatcgcc tttaaagcgg
     9421 acattggaaa actcggtgcc acccaccaga cccggttcga tgtcggtgac gcgcaccgcc
     9481 gtaccatgca ggtccgtacg cagattcagg ctaaattgac gaacaaacgc tttcgtcgca
     9541 ccgtaaacgt taccaccggc atacggccag ctaccagccg ttgagccaat gttaataata
     9601 tgaccatgat tacgttcaac catacccggt aagacggcgc gcgtcatata taccaggcct
     9661 ttgttgttgg tatcaatcat cgtttcccag tcttcaatgc tggctttatg cgcaggctcc
     9721 atgcccaacg ccaggccggc attgtttacc aggatatcaa tattgctcca ctcggcaggg
     9781 agcgatgcca gcatctcttc aatagcggcg cggttgcgaa cgtccagttg ggcgatatac
     9841 agattatctc ccagttcgtc ttttaactcc tgtaaccgct cctggcgacg gccagtggcg
     9901 ataactttat gcccttgttg aataaaacga cgagtaatgc attcaccaaa acctgccgtt
     9961 gctccagtta ctaaaacgat catctcactg ttcctcaacg cttttgtgtg tcaataacat
    10021 agcacgtgat gcttaagagc gggtaatact ggtttgcaag attgcactcc gttatcagta
    10081 agcctacgct atggaacacc tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc
    10141 cttgtgcaaa gcacactgcc gtatctggct ccccattttg atcaaattgc caaccatcac
    10201 tatcgcccgg cattcgatga gggaatacag caaaagcggg cagaaatagc tgccatcgcg
    10261 cttaacccgc aaacacctga tttcaacaat actattctgg cactggaaca aagcggagaa
    10321 ttacttaccc gcgttaccag cgtctttttt gcgatgactg cggcgcatac caatgatgaa
    10381 ttacagcgtc ttgatgaaca gttttccgct gaactggcgg aacttgcgaa tgatatctat
    10441 ctgaacgggg aattattcgc gcgggtagat gctgtctggc aacgccgtga atccctgggg
    10501 cttgatagtg aatccatccg cctggtggag gtgattcatc aacgttttgt ccttgccgga
    10561 gccaaacttg agcaagctga taaagcaaaa ttaaaagtac tgaatacaga agctgcgacc
    10621 ctgaccagcc agtttaacca gcggttactg gcagcaaata aatccggcgg tctggttgtg
    10681 aacgatgtcg cgcagctggc aggaatgagt gaacaagaga ttgcgcaggc ggcagaggcg
    10741 gctcgcgaga aaggtctgga taacaactgg ctgattccgt tgctgaatac cacccaacaa
    10801 ccagcgcttg ctgaactgcg cgatcgcgcg acgcgtgaaa aactgtttac tgcgggctgg
    10861 atgcgagcgg aaaaaaatga tgccaatgat acccgcgcta tcattcaacg tctggtggag
    10921 atccgcgcgc agcaggcgaa actgcttggt tttcctcatt atgccgcatg gaaaatcgcc
    10981 gatcagatgg caaaaacgcc agaagcagcg ctcaacttta tgcgggaaat tgttccagcg
    11041 gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg cggttatcga taagcagcag
    11101 ggcgggttta gcgcgcagcc gtgggactgg gcattttatg ccgagcaggt aagacgtgag
    11161 aaattcgatc tcgatgaatc gcagctcaag ccatattttg aattaaacac ggtgttgaat
    11221 gaaggcgtat tctggactgc gaatcagctc ttcggtatta agtttgtcga acgttttgat
    11281 attcctgtct accatcctga cgttcgtgtg tgggaaattt ttgatcataa tggcgtgggg
    11341 ctggcgttat tttacggtga tttcttcgcc cgtgattcaa aaagcggcgg cgcatggatg
    11401 ggcaattttg ttgagcaatc aacgctgaat gaaacgcacc cggtaattta taacgtctgc
    11461 aattatcaga aacccgctgc cggtgagcct gcgttgttac tctgggatga tgtaataacc
    11521 ttattccatg aatttggtca tacgctgcac ggcctttttg cccgccagcg ttatgccacg
    11581 ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc cgtcgcaaat caacgaacac
    11641 tgggcaacac atccgcaggt attcgctcgc tacgcccggc attatcagag cagagccgca
    11701 atgcctgacg aacttcaaca gaaaatgcgt aatgccagcc tgttcaataa agggtatgag
    11761 atgagcgaac tgcttagcgc cgcacttctc gatatgcgct ggcattgtct ggaagaaaac
    11821 gaagcaatgc aggacgtcga tgatttcgaa ttgcgggcgc tggtggcgga aaatatggat
    11881 cttcctgcta taccgccacg ctatcgcagc agttatttcg cccatatttt tggtggcgga
    11941 tatgccgcag gttattacgc ttatctgtgg acgcaaatgt tggccgatga tggttaccag
    12001 tggtttgttg agcagggcgg attaacgcgt gaaaatgggc ggcgttttcg cgaggcgatt
    12061 ctttccagag gtaacagcga agatctggaa cgcctgtatc gacaatggcg cggtaaggca
    12121 cctcagatta tgccgatgct gcaacatcgt ggcttgaacg tataaacacc accaatggct
    12181 ggcctggatt tatcgccaga ccagcttcgt tatagcagta attgcagcaa ctgggcgagg
    12241 gcaaacttca ccgctaatgc gaatgccgtt tcgcagtcgc cagcaaaatt actttttttg
    12301 tatactcttc cgttataata attttcaaat tcagccggac cagatgatca acattagcgg
    12361 cagtctggat aagaaaagcg cgccggccgt cgttcgggtc actcagttgc agaaataaaa
    12421 ataaattagc ctgatggcct gaac
//
LOCUS       DAZFRG010000142        12234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFRG010000142 DAZFRG010000000
VERSION     DAZFRG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.142"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1637
                     /gene="sapA"
                     /locus_tag="ACHKUC_003192"
     CDS             <1..1637
                     /gene="sapA"
                     /locus_tag="ACHKUC_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO6619404.1"
                     /translation="VLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTF
                     NPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVP
                     FQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKL
                     DNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPYQLS
                     EYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLS
                     ILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAE
                     TAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLK
                     TAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLS
                     CAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLASSL
                     RLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            1634..2599
                     /gene="sapB"
                     /locus_tag="ACHKUC_003193"
     CDS             1634..2599
                     /gene="sapB"
                     /locus_tag="ACHKUC_003193"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO6619405.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            2586..3476
                     /gene="sapC"
                     /locus_tag="ACHKUC_003194"
     CDS             2586..3476
                     /gene="sapC"
                     /locus_tag="ACHKUC_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO6619406.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            3476..4468
                     /gene="sapD"
                     /locus_tag="ACHKUC_003195"
     CDS             3476..4468
                     /gene="sapD"
                     /locus_tag="ACHKUC_003195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO6619407.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            4470..5276
                     /gene="sapF"
                     /locus_tag="ACHKUC_003196"
     CDS             4470..5276
                     /gene="sapF"
                     /locus_tag="ACHKUC_003196"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO6619408.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(5324..6487)
                     /locus_tag="ACHKUC_003197"
     CDS             complement(5324..6487)
                     /locus_tag="ACHKUC_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO6619409.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(6496..7869)
                     /locus_tag="ACHKUC_003198"
     CDS             complement(6496..7869)
                     /locus_tag="ACHKUC_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO6619410.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALFGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(7873..10980)
                     /locus_tag="ACHKUC_003199"
     CDS             complement(7873..10980)
                     /locus_tag="ACHKUC_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO6619411.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYVVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(10980..12107)
                     /locus_tag="ACHKUC_003200"
     CDS             complement(10980..12107)
                     /locus_tag="ACHKUC_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6619412.1"
                     /translation="MKYIATSVVAMLLLSGCDNTHTQSNNSSPSETEVGVVTLKSQPV
                     SVVSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEA
                     RAALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALET
                     ARINLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLL
                     RLRKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQ
                     LLPGMFVRARVDEGVMENAILAPQQGVTRDAKGNANALVVNKDNKVEQRTLETGETYG
                     DKWLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
BASE COUNT         2903 a         3296 c         3078 g         2957 t
ORIGIN      
        1 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
       61 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
      121 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
      181 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
      241 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
      301 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgatgatg
      361 tggtgtttac cttccaacgg atttttgacc gcaacaaccc gtggcataac gtcaacggca
      421 gtaacttccc ctatttcgac agtctgcaat ttgccgataa cgtgaaaagc gtccgcaagc
      481 tggataatca taccgttgag ttccgtctcg cccagccgga tgcttctttt ttgtggcacc
      541 tggcgaccca ttatgcttcg gtcatgtcgg cagaatatgc agggaagtta gagaaagaag
      601 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
      661 accgcgccgg gcaatatatt cgcttacaac gtcatgatga tttctggcgt ggtaagccgt
      721 tgatgccaca agtggtggtg gatttaggct ctggcggtac aggacgattg tcgaaactcc
      781 tgaccgggga gtgcgacgtt ctggcctggc ctgctgccag tcagctatcc attttgcgtg
      841 acgacccacg cttgcgttta acgctgcgtc ctgggatgaa tgtcgcctat ctggcattca
      901 acaccgccaa accgccgcta aataatccgg cggtccgcca tgcgctggca ctggcgatta
      961 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggct gcctctattt
     1021 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccagcaa
     1081 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
     1141 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac cgccgaactg attcaggcgg
     1201 atatggcaca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgt tttcaggagg
     1261 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
     1321 acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc
     1381 tcgcccactg gtgcgatccg aaattcgaca gcgtattgcg taaggcgctc tcttcgcagc
     1441 agctggcggc gcgtattgaa gcctatgacg aagcgcaaag tattctggcg caggaattac
     1501 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtatgat atcaaaggtc
     1561 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
     1621 aggtgaaaaa gccatgatta tcttcacctt acgccgtatt ttgttattga ttgtgacctt
     1681 gttcctgctg accttcgttg gcttcagctt gagctatttc accccacatg cgccgttaca
     1741 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
     1801 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
     1861 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtagg
     1921 aatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
     1981 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ctgacgctgt tttgttcact
     2041 cacactaggt tggttgcccg tttctgggcg tttcgatttg ctctacgaag tgaaaccgat
     2101 taccggtttt gcgttgattg atgcctggct ttcggactca ccgtggcggg atgaaatgat
     2161 cattagcgca atccgtcata tggtattgcc cgtgatcacc ctgtcggttg cgccaacaac
     2221 tgaagtgatc cgactaatgc gtatcagcac tatcgaagtg tatgaccaga attatgtaaa
     2281 agcggcggcg acccgcggtt tgtcacgctt tactattttg cgtcgccacg ttctgcataa
     2341 cgcgttacct ccggttattc ctcgcctggg cttacagttt tctaccatgc tgacgctggc
     2401 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
     2461 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
     2521 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
     2581 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
     2641 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgtgcggggc
     2701 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
     2761 tcggttatca attattgccg ccgtcatggt cgcgctatgg cgaagtttct ttcttcctgg
     2821 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
     2881 taggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
     2941 tcgccggggc gacccacggc ctacgctcag cggtgcttaa ccatattctg gataccttac
     3001 tggcgatccc ttcgctgctg ctggcaatta tcgttgtagc gtttgccgga ccgagtttgt
     3061 ctcacgccat gtttgctgtc tggctggcgc tgctgccgcg tatggtacgt tcgatttaca
     3121 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
     3181 caacgctgaa tattctctgg tttgctgtga tgcccaacat caccgccggg ctggtcaccg
     3241 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
     3301 tcggcgcaca actcccctca cctgaatggg gcgcgatgct cggtgatgcg ctggaactga
     3361 tttatgtcgc cccatggact gtcatgctgc caggtgcggc aattatgatt agcgtgctgt
     3421 tggttaacct ccttggcgac ggtgtccgtc gtgcaattat tgcgggggtg gaataatgcc
     3481 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
     3541 cgtcgaccgc gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggcgaatc
     3601 cggttccggc aaaagtttga ttgctaaagc gatttgtggg gtgaataaag ataactggcg
     3661 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
     3721 acggcgcaag ctggttggtc acaacgtgtc gatgattttc caggaaccac agtcgtgtct
     3781 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atccctgcct ggacctataa
     3841 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
     3901 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
     3961 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctaattgc
     4021 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
     4081 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
     4141 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacggtgg aaaccgcgcc
     4201 gagtaaggag ttggtgacga tgccacacca tccttatacc caggcgctga tccgcgcgat
     4261 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
     4321 cccgctgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
     4381 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
     4441 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctacttgaa gtgcgtaatt
     4501 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
     4561 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattatcggc gagaatggtt
     4621 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt
     4681 tattgattga cgatcatccg ctgcattttg gcgattattc cttccgcagt cagcgtattc
     4741 gcatgatttt tcaggatcct tccacctcgt tgaatccccg tcaacgtatc tcgcaaattc
     4801 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
     4861 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
     4921 tagcacccgg gcaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag
     4981 tcattattgc cgatgaagcc ctcgcctcac tggatatgtc gatgcgttcg cagttgatta
     5041 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
     5101 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
     5161 agcgaggcag caccgcagat gtgctggcat cgccgctgca tgaactcacc aaacggctga
     5221 tcgccggtca ttttggcgaa gcattgacgg cggatgcgtg gcgtaaggac cgttagtgtt
     5281 ttgcacctca tcctgacagg cccccctcag gatgaggtgt tcattacttt tgattgtcga
     5341 ttctggtgag agcaaccagc aatgccagcg tataacacac cgtaattgcc agactcattt
     5401 ttaacagtgt ttctccgcca attcccgaca atggcgcggc tataccgccg aagacaaaca
     5461 tgctcatccc catcaacgca gacgccgttc cagattcctg cgtccctact gcactcatag
     5521 cctctgcccc gccaacagtg ccgatgccgc tacaaaatgc gatggtgaaa aataatgcca
     5581 ccagtgccgg aatgggcaat tgtgtccatg cgcataagac cgtgagcaat gcacacaaaa
     5641 ttgccgctat caggccacct cgcaacaatg tcatcgcgtt aatccgtcgc gccaggcgcg
     5701 agaagatcca tgaagcaatg ataagtccga tgccgttaag gccaaacacc aggctaaatt
     5761 gcattggact aaagccaaat tccttctgca acacgaacga cgaagagccg atatatgcaa
     5821 acaaaccggc cagcataaaa ctttgaatca ggcaaaagcg cataaaacgg cggttctgca
     5881 ccacgcttcg tccggttaat agcaatgatg agcccctttt attttctggc aaggtctcat
     5941 taataaataa caggcagccc agcaacagta cggtgctaat ttcagccatt acccagaata
     6001 aagtgcgcca gtcaaaagtg ctaacaatgt acccgcccag cactggcgac aacaccggtg
     6061 ccaggccatt caccgtcatt aacagcgcaa aaaactgggt caacgttact ccctgatatt
     6121 tgtcacgagc aatagaacga gagagcaccg aaccacccgc ccccgcgatc ccttgaataa
     6181 aacgccagac caccagccag taaatattgt tcgttgtcgc gcacaaaatg gaagaaacaa
     6241 taaacaatag caacgacaag attaacggac gtttacgccc tattttgtcg ctcagagggc
     6301 caaataacag ttgtccgacg ccaagaccaa tcagtgatgc ggtcagggtt aattgcgtta
     6361 tggtcgtggt tgccgccagt tgctcagaca tctccggcaa agccggcaaa taaagatcag
     6421 tacacatcgg gccgataccg gctaaaagac caagaatcaa tgcccatgaa agagagactc
     6481 tagccatatt attccttact tgagtgatga catgccgccg cccagcgact gccagagcgt
     6541 gattcggttt gtaaaatcag tcagttgcag tgccagcagc tcctgctgtg cgctccacaa
     6601 gctgcgctgc gccgtcaaca cggtcaggta atcaccaaca cccgcctgat agcgacgcaa
     6661 gccaacatcg accgtttgtt gttcagcttt aacatactgg cgctgtgcat ccagttgctc
     6721 ttcaagcgtg gtccgccgcg ccagtgcgtt agcgacatct ttaaatgcgc tctgaacggt
     6781 tttttcatag gtggcaatga gtccacgctt ttgtgcttct gcgtaacgaa gctgcgccag
     6841 attacttccg ccagtaaaca gcggtaatgt gaccgatggc gcaaatgacc agatctgcat
     6901 tccgtggctg aacaacgacg acaacgcgtc actgccaaca ccggcactgg cagtcagcga
     6961 aatcgtcggg aaaaagttag cccgcgccgc gccaatatct gcattggcgc tcttcaaatt
     7021 atgttccgct tcctggatat ccgggcgacg cagcagaaca tcagatgata ctcccgcagg
     7081 aaccagactt atcatttgtt ccggcaggct ttccagtgtt cccggtagca gattttccgc
     7141 aagcgttgta cctgccagta agttcagcgc atttttatcc tgcatcactt gtgtctgata
     7201 gctggcaacg ctggcgcgcg cttgctgata aacactcatc gcctcactga catctgtcgc
     7261 tgccgccgtg ccaacctgtt gctggcgctg gataattttc aatgagtttt ctgcactggc
     7321 catcgtttct tttgccaggg ccaggttgct gttatcagcc gccagtgtca gccaggcggt
     7381 gctgatttca gcaatcagcg ttaaacgagt gttctgggct gtaaattcac tggcaagcca
     7441 ggtttcccgc gccgcccgag ataaactctg attacgacca aacagatcta acgtataact
     7501 ggacaccgtg ccgtcagcct cggcagttgt tcccgtacca ttcgccagcg aacgactgcg
     7561 agtcgaactt aaggccgcat tcaccgtcgg aaataacgac gcattagttt gcccaaagag
     7621 cgcccgtgct gagtcaatat cagcaatcgc tttttgcaca tcgcggttac tgttcagtgc
     7681 aatggttact acctgctgca atcggggatc gtggatcacc tgctgccagt catggctgat
     7741 tgccttcccc tggccctgtg ctcccggcaa ggtcgccggg attggggatt caggcgtact
     7801 gtagtgagga tcgagcgaga cacatcccgc actcaacaac accagaaaaa taagagaacg
     7861 acgcaacata acttactcct gacggcgcgg tttaccggca aacaaacgtt tcaccagtac
     7921 aaaaaacaga ggaacgaaga aaatggccag taatgtagcg gtcagcgtgc cgccaataat
     7981 gcccgtacca atggcgatgc ggctgttcgc ccctgcgccg gttgctatcg ccagcggcat
     8041 tacccccgca ataaacgcca gcgaggtcat gatgattggg cgtaaacgag tctgcgccgc
     8101 gcgtaatgcc gcacgactca aggaatagcc ctctgcaacc gccgcttcag caaattccac
     8161 aatcagaatg gcgtttttcg acgacaagcc gatagtggtt aatagcgcca cctggaagta
     8221 aacatcgttg tttaaatcac gcatccaggc cgccagcgcc gcgccaagaa gccccagagg
     8281 gatcaccaga atgaccgaga acgggactga ccagctttcg tacagtgctg caaggcagag
     8341 gaaaaccacc agaatagaaa ctacatacag acttaatgcc tgaccgctgg ccagtttctc
     8401 ctgcaatgac aaaccactcc aggcccaggt tgttcccgca ggaagactgt ttgccagttc
     8461 ctccattttt gtcattgcat cgccggaact aaagccagtc gcgttttcgc cctgaatttc
     8521 ataggctgcc gagccgttgt agcgcaccag tctttccggt ccatacagcc agcgggtggt
     8581 cgcaaaagca gagaatggtg tcatggcgtt atcgctaccg cgcacaaacc atttacctaa
     8641 gtctgacggc gcggaacgaa attcgctgtc gccctgaatg tagacttttt tcacacgacc
     8701 acggtcaata aagtcattca cataagtacc gccccacgca ctggacaggg tgtcggtgac
     8761 atcattcaaa ctcaacccta atgacaccgc tttattgcta tcaatatcta cctgcaattg
     8821 cggcatttgt ggcaaatcat tggcgcgtac agaatgtaac tccggacttt gattcgcttt
     8881 ttctatcaac tgattacgca tttgcagcag tgtttcacga tcggttccac cgttagctaa
     8941 caattcaaac gtaaaaccat tgctttgccc cagcccatca acggctggcg gcgtcatcgc
     9001 aaataccgtg gcatcacgaa ttgtgcccag ctctttggtt gcccgtaggg cgatagcctg
     9061 ggcggtgttt tctgcccctt tacgttgaga ccagtttttc aacgaaacaa acgccatccc
     9121 ggtgttctgt ccgctgccgc taaaactgaa accatcaacg gtaaagatga catcggtatt
     9181 tgctttctcg ttaatcagga accagtcaac aatctggcga ttcacttctg ctgtacgggc
     9241 agcggtcgcc ccggccggca gcgtgtactg caccatgatt tcgccctggt cttcagtggg
     9301 taagaaactg cccggcagtt tccacatcat cagagccatt ccaccgccca gcacgacata
     9361 aagccccatc gttcgggctg cacggcgcag gacataaatt acgcctcgct gatatttatc
     9421 ttcagtacgg cgataaaagc ggttaaatgc gccgaaaaag ccttttttat gtggcggaac
     9481 atgctggagg acggaaccgc acagggcggg agtcaaggtc aatgccacca ccacggaaag
     9541 cagcattgcg gagatgatgg tgatggagaa ctggcgataa attacgcccg tagatccccc
     9601 aaagaaggcc atcggcagga atacggctga caacaccagc gcaatggcaa ccagtgcgcc
     9661 agaaatctcg cccattgatt tttccgtggc ttcacgcgcg ggcaaccctt cgtcacgcat
     9721 aatgcgttcg acgttctcta ccaccacaat ggcatcgtcc accagcaagc cgatcgccag
     9781 caccattgca aacaacgtca gggtattaat cgaatagcca aacaacgcaa gaacgccgaa
     9841 tgtgcccagc aaaacgacgg ggacagctaa cgccggaatc agtgtggcac gcaggttttg
     9901 caggaataaa tacatcacgc aaaccaccaa tacgatggct tcaaacagcg tctgaattac
     9961 atcctctacg gagattttaa taaattcagt gctgtctttc gggtatgcga tgtcgtatcc
    10021 ctgcggcatg ttccgctgga attcggcaat cttatccttg accagcgtcg ccgtattcag
    10081 cgcattcgca ccgggcgaaa gcattacagc aaccccggca gacgggtgac cgttaagttt
    10141 gccaatagcg gtataatctt cactgcccat ctcaacgcga gccacatctt ttatacgaac
    10201 aactgcgccg tctgactggc ttttcacgat aatatttttg aactgatcca ccgtctgcaa
    10261 acgagactgc gcacgtaccg ttgcggtcag ttgctgagta tccggtgaag gcaatgcccc
    10321 tattttcccg gcagtaacct gcacgttttg cgcttcaata gcactttgca cgtctgaagg
    10381 catcagcgag taagacgcca gtttggccgg gtcgagccag atgcgcatag cgtattccgc
    10441 gccaaagact tgcagactcc ccacaccttc aacacgcgcc agcgggtcct gaacgttact
    10501 gaccagccag tcggcgatat ccgaactgga ggctttgtca gtggtgtcat acacggcagc
    10561 aatcagcaaa aagttgcttt gtgacttctc caccgtaaca cccgtttgct gcacttcgct
    10621 gggtaggcgc gactccgcct gctgaatttt attctgcacc tgaacctgtg cagtatctgg
    10681 atcggtacct tgttcaaagg tcacattaat actgaccgaa ccatcagagc tactggttga
    10741 gctgaaatag agtaaattat caagcccagt gagttgttgc tcgataacct gagtcacgct
    10801 gttctccagc gtttcggcag aagcaccagt ataagtggct gaaattttaa tggtcggcgg
    10861 tgcaacgtca ggatattgcg cgacgggcaa tgtgcgaata gccagaatcc ccgccaacat
    10921 aatcaaaatg gcgataaccc aggcaaagac tgggcggcga acaaaaaagc gcgaaaacat
    10981 caggcattgc ctccattagc ctgaacttca acagccttga cggtctgccc tgaagtgact
    11041 ttggcagaac cttcaacaat cagtcggtcg ccgttgtgca ggccgttcag caccagccat
    11101 ttatcaccat acgtttctcc cgtttcgagc gttcgctgct ctactttatt gtctttattc
    11161 accaccagcg catttgcatt gcctttagca tcgcgcgtaa cgccctgttg aggcgcgaga
    11221 atagcgtttt ccatcacgcc ttcatcgaca cgagcgcgaa caaacattcc cggtaataac
    11281 tgctgttgtg gattggggaa aattgcccgt aatgtcaccg aaccggtaga ctcatctaca
    11341 gcgacttcgg tgagttccag acgccctttt tcgctgtagg ttgtgccatc ttccagaata
    11401 agtgagacgc tcatggtgtc actgttggtc gccagtgact gtttacgcag acgtaataaa
    11461 tcgacactgg agcgagtgag gtcgacatac attgtatcca gaccacgaat agtcgttaac
    11521 gctgtatctt gcgacgcggt caccagtgcg ccaggggtta ccgacgaaat gccaatgcgc
    11581 cctgaaatcg gtgcggttac cgtggtccag tcaagattaa tgcgcgcagt ttccagtgcg
    11641 gcttttttcg cctctacact ggctttatct tgtgcacagg tagactgagc atcatcagca
    11701 tcctgttgtg aaacaccgtt ctctttcacc agtcgggcat aacgctgcgc tttctggcaa
    11761 tcggctttta ccagtgcctg tgcttgttgt aatgctgccc gggcttcatt ccatgcagcc
    11821 tgataactgg ccgcatcaat ctggtagaga ggctgtccag ccttgaccag atcaccttcc
    11881 ttaaataagc gtttctggat aattccccca acctgcggac gtacttcagc actgagcgca
    11941 gcactggtgc gtccggttaa ttcactgact accgaaaccg gttgagattt gagtgtaaca
    12001 acgccgactt ctgtttctga cggggatgaa ttgttacttt gcgtatgcgt attatcacaa
    12061 cccgataaga gcagcattgc cactacagat gttgctatat acttcattat tttctccagg
    12121 gtgagaaata ccctcctcat tgaaattaat gaggatatgt cagggatatt cagttataac
    12181 gaacccgcat cgcgaaatga atttcgcaat gaaataaggg ggattaatat cccc
//
LOCUS       DAZFRG010000143        12093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFRG010000143 DAZFRG010000000
VERSION     DAZFRG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.143"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..1399
                     /locus_tag="ACHKUC_003201"
                     /pseudo
     CDS             52..1399
                     /locus_tag="ACHKUC_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
     gene            1643..2609
                     /locus_tag="ACHKUC_003202"
                     /pseudo
     CDS             1643..2609
                     /locus_tag="ACHKUC_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2953..4251
                     /gene="kgtP"
                     /locus_tag="ACHKUC_003203"
                     /pseudo
     CDS             2953..4251
                     /gene="kgtP"
                     /locus_tag="ACHKUC_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
     gene            complement(4248..4571)
                     /gene="yfiM"
                     /locus_tag="ACHKUC_003204"
     CDS             complement(4248..4571)
                     /gene="yfiM"
                     /locus_tag="ACHKUC_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO6619413.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(4617..5972)
                     /gene="pssA"
                     /locus_tag="ACHKUC_003205"
     CDS             complement(4617..5972)
                     /gene="pssA"
                     /locus_tag="ACHKUC_003205"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO6619414.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(6086..8746)
                     /gene="pat"
                     /locus_tag="ACHKUC_003206"
     CDS             complement(6086..8746)
                     /gene="pat"
                     /locus_tag="ACHKUC_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO6619415.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIARLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLSDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAKQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(8778..9476)
                     /gene="tapT"
                     /locus_tag="ACHKUC_003207"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(8778..9476)
                     /gene="tapT"
                     /locus_tag="ACHKUC_003207"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO6619416.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(9545..9964)
                     /gene="trxC"
                     /locus_tag="ACHKUC_003208"
     CDS             complement(9545..9964)
                     /gene="trxC"
                     /locus_tag="ACHKUC_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO6619417.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            10171..11208
                     /gene="yfiF"
                     /locus_tag="ACHKUC_003209"
     CDS             10171..11208
                     /gene="yfiF"
                     /locus_tag="ACHKUC_003209"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6619418.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(11256..11945)
                     /gene="ung"
                     /locus_tag="ACHKUC_003210"
     CDS             complement(11256..11945)
                     /gene="ung"
                     /locus_tag="ACHKUC_003210"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO6619419.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTALGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLLAESE"
BASE COUNT         3024 a         3017 c         3176 g         2876 t
ORIGIN      
        1 tgaactgcac cccaaatgtt ggacataatc tgacatctga aggtgcagtt tatgaaacat
       61 tcatttgaaa taaaacttgc cgctgtaaat cattacctgg ctggtcatgc aggcatcatt
      121 tctacggcaa aactctttca actttctcac accagcctct cacactggat taatcttttt
      181 cttcttcacg gtcctcggca ctggattgca gacacaagcg tagctattct cctgaagata
      241 aactttgcgt ggttctttat gctctcgggc attctgagtc tctaccccgg gtagctgccc
      301 ggtttaacat tcccagccac aacacggtta aaaactggat aaaaggctac cgcaaatctg
      361 gtaatgaggc ctttatcagg cgcaggaagg agaaaagcat gacccgttct gatgataccc
      421 atgaaaacga ggcaaacatg acgccggaag agatgaagaa tgagctgcgt tatcttcgcg
      481 cagaaaatgc atatctgaaa gccatgcagg agcatcttct ggaaaaaaag cgccaggagc
      541 tggagaaaaa acgaaagtca tccagagcct gaggtacgga cactgccagt ctgacctgtt
      601 aaaggccgca gggctggcca gaagcacgct gtactaccag ctgtcactgc agaaggctaa
      661 ggacaagtat gctgatgtga aacagctcat agcctccatc ttccatgaac accggggatg
      721 ttatgggtac cggcggatcc attgtgagct tcagaaaaga ggtcttaagt tcagtggcaa
      781 aacggtgaga aaactgatgc aacagctcgg cctgaagtct cctgtgcgcc tgaagaaata
      841 ccggtcttac cggggaaata tgggactcgc tgcagaaaat atccttcagc gacagttcaa
      901 agcggaagct ccctgtgaga aatgggtgac agatatcacc gaattcaggg ctggcggaca
      961 aaaactgtac ctgtccccga tacttgattt gtttaacggg gaaatcgtgg cgtgggaaac
     1021 ggcctgcagg cctacagagg agctggtgaa gcggatgtta aataaggggc tggaaagcct
     1081 tgctgaaggg gagaaaccgc tgctccacag tgatcaagga tggcattacc ggataaaaag
     1141 ttatcagtcc gccctggcgg acagagggtt agtgcagagc atgtcgcgca agggcaactg
     1201 ccttgataat gcggtaatgg agaacttctt tggtcacctg aaagaagaaa tgtactatcg
     1261 ccgggactac agaaatgtag aagagctgga aaatgccgtt aacgaataca taacttactg
     1321 gaatcaaaaa agaataaaac tcagtcttgg gggactcagc ccggtagaat accggactga
     1381 gtatcaaaaa gccggttaac tgaaactgtc caggttttgg ggttcagttc atgccgcatc
     1441 ttatccgacc ttgtattatc cctccagtgc agagaaaatc ggccagtttt ctctgcctgc
     1501 agtccgcatg ccgtatccgg ccttgggttc taacctgcat ggtagattta tgcggcggac
     1561 tgcctttctc ccgaagtgat aaaccggaca gtatcataga ccggttttcc cggtaatccg
     1621 tatttgcaag gttggtttca ctatggaaca tgaacttcat tatatcggta tcgacaccgc
     1681 taaagagaaa ctggatgtcg atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt
     1741 cgctaacacc actaaagggc acgatgagct ggtgagttgg ctgaaatgtc acaagattga
     1801 ccatgcgcat atctgcatcg aagcgaccgg cacctatatg gaacctgtcg cagagtgcct
     1861 ttacgatgct ggctacatag tgtcagtcat taatcctgca ctgggtaaag ctttcgctca
     1921 gagtgaagga ctgcgtaaca agactgatac cgtggatgcc gcatgctggc agagttctgt
     1981 cgtcagaagc gccctgcagc ctgggaagcg catcacccgc ttgaacgcgc gttgcatgcc
     2041 ctggtagtac gccaccaggc gctgacagat atgcacaggc aggaactgaa tcgcactgaa
     2101 acggcgcggg aagtccagag gccgagcatt gatgctcacc ttctgtggct tgaagcagag
     2161 ctgaagcgtc ttgagaagca gataaaagac ctgacagacg atgatccgga tatgaaacac
     2221 cgcaggaaac tgctggaaag catcccgggt atcggagaga aaacatctgc ggtattgctg
     2281 gcttacatgg tctgaaggac cgttttgccc atgccagaca gttcgccgct tttgcgggtc
     2341 tgacaccacg gcgttatgaa tcaggcagta gtgtgagagg ggcaagccgg ttgagtaaag
     2401 ccggacatgt gtcgcttcgc aggccgttgt atatgcctgc aatggtagcc accagtaaga
     2461 ctgagtgggg acgggcgttg gcagctaatg gcaagaaagg aaaggtgatt ctcggctcga
     2521 taatgcgcaa gctggcacag gtggcgtatg gagtgctgaa gtcaggcgtg ccgttcgatg
     2581 cgtcacggca taatccggta gctgcgtaaa aatcgcggaa gggatgaaac aacagcgcct
     2641 gacggcgctg tgtctggcat gcctgcaatc cgggagactg gaccaggaaa aaacttgctg
     2701 gccgtaacag tatctacgct cccacataag ccagattcaa cagcgaatac gtcttcccca
     2761 aattgcccac ttccatactt cctccttacc agaaatctat ccttaagctc cttaataacc
     2821 attttcctgc taactaaatt catggttaag gttgcataat gatatgcaac aaatgtataa
     2881 cattttttct taccaaaaaa taaacaaaag cgaccgacaa aagcattgga ttacggcagg
     2941 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
     3001 actcgtcgcc gcatttaggc aattgttggg gcctcttcag gtaatctggt cgagtggttc
     3061 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
     3121 aacacgacga ctcaactact gcaaacagca ggtgtttttg ctgcaggatt tctgatgcgc
     3181 ccaataggcg gttggctgtt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
     3241 ctgttatcgg tgtgtatgat gtgtttcggc tcactggtta tcgcctgttt gccaggctac
     3301 gaaacaatcg gtacctgggc tccggcatta ttgcttctcg cgcgtttatt ccagggatta
     3361 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
     3421 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcgggca actgctagct
     3481 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgtactcag agagtgggga
     3541 tggcgtattc ctttcgcgtt aggggctgtg ttagctgttg tggcgttgtg gttacgtcgt
     3601 cagttagatg aaacttcaca acaagaaaca cgcgctttaa aagaagctgg atctctgaaa
     3661 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     3721 ctttgtttct ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg
     3781 catgccaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     3841 ccacttattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     3901 tctctggcaa ccatttttac cgttcctatt ctctcagcat tgcagaacgt ttcctcgcct
     3961 tatgccgctt ttggtctggt gatgtgcgcc ctgctgatag tgagttttta tacttcaatc
     4021 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggtctg
     4081 tcatatgcgg tcgccaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     4141 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     4201 tttctggttt cactaacatt gcaccgtaaa ggaaaaggga tgcggcttta gtgacgggtc
     4261 agttgccaga cggtatagcc ggtgcttgca ccggcgatat cccaggcaaa atccttccag
     4321 ctccagccgc tcccctcagg gcggctatcc caaagctctt ttgacgcccc cagactgaca
     4381 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     4441 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     4501 ctccagttat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     4561 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     4621 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     4681 accttcaccg gataatcggc aatactttgc agatcgcggt agtgcttaac gatggtggta
     4741 tgctcgcgga tcagatccag ttctttctct cgctgtggcg caagctccag ttgcggatcg
     4801 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     4861 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     4921 tctttccata accgaactac taactggtca gtgttgacgt aatactgtaa acgactcagg
     4981 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     5041 tcttccggaa tgtagaagtc attcgcggtt ttatcaccaa caataatttc gacctttttc
     5101 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     5161 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     5221 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     5281 ccctggaaat gataagcggc atcacgcagc tcctggcgga acaggcgaat gtcgttcttg
     5341 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     5401 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gatcagatga
     5461 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     5521 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     5581 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg
     5641 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     5701 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcctcatac
     5761 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc aacaatgcaa
     5821 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     5881 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     5941 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     6001 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     6061 gtggtcaaca tttccagtac cttactcatg attcctcgcg ctgggcaaga tttagcgtaa
     6121 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggccagcg
     6181 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     6241 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     6301 agcgaaccag cacggcaaat tcggcatcga tgttatcagg gtcggagatc gcacgtgtga
     6361 cgccgaggat ctcttccgtt tgatcaattc gtcgtaccgc tacaaatgcc atttcccgat
     6421 cgtagtcgat ctgcgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     6481 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agctgtggct
     6541 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     6601 cttccagctg atgcggataa gggcgcacag ccagccggct ctcgttatca ccttcaaacg
     6661 gcgcgatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     6721 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     6781 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgtgcacga atctttttac
     6841 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     6901 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg attaacggcc
     6961 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     7021 tactttgcac caacaggccg tggacccgcg cctgcggcca ggccattttt acgcgatcga
     7081 aaatagcgtt tgccgcttgc tggacttcat tggctgtacg taggtagagc atgacgccct
     7141 gaacttccga tttatgtgga atatccggcg agcgcaattt cagtgccacc ggatagccaa
     7201 tctgtttggc aatatgtacc gcttcggtgc tatcactggc aatccaggta gggagagtat
     7261 tcataccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     7321 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     7381 gcaacgccgg cgtttcgcgt agttgcttct gattacgtcg gtactcgacc atatgcataa
     7441 aagcagtgat ggttccttcc ggagtacggt aggtcggcag cccggcttcg ctgaataaac
     7501 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     7561 tgcggggatg atgctttacc gcttcaatta atacttgtgc gctttctgtt gcgggagcag
     7621 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     7681 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgagc gggttagaga
     7741 ttgccacatg ccctggcagt gcatcgctca gtttctggca ggtttcttcg cttagcgttg
     7801 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     7861 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     7921 ccgaaaacag ttcgtgggtg tcctgtaccc gcaacaaacc ggcacgctga atagctgcat
     7981 cccacgccgg atccattcct gccgtggtgt tcaacagacg ttgagccgcc gggctacgtc
     8041 cgcttttaat caccaaaatc ggtttattgc gcgaggcact acgggctgcc gaaacaaagc
     8101 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     8161 ccaaatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     8221 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acagcagccg
     8281 attgcgaaat aaacgccagc ttgccgcgtt taatcggcac aggtgaaaag ctggcattca
     8341 gaccttgcca gggagccagt aagcccagac tgtttggtcc tagcaggcgc atgttatggc
     8401 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     8461 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     8521 ataaaaccgc aaggtcgggt gtaaagggca agcgggcaat atccggccag gccaacacac
     8581 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cctcccgcca
     8641 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgatgcg ccaattaccg
     8701 caatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcatatcg gtctcccgca
     8761 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct tcccggatgt
     8821 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgtc
     8881 gccgccctgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     8941 tggccttcag cctgggcttc acgcaggcga taggcagaaa gccgagaaag atcgacggaa
     9001 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttctggccag
     9061 gtaccatcga gcatgataaa tagcggaggc ttaccggcag gtggtgtgaa gatcacttcc
     9121 cgttgctcat cagcatacga agcgggaaag accaccattg gctgatagtc cgggttttgc
     9181 accagatcca gcaaatcttg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     9241 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     9301 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     9361 ttctctggca aaagacagcg ttggcagcga cgaacgcgat taccgcgggc aagaaaagga
     9421 cgtgttgcac gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     9481 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcgccccggt
     9541 aagattaaag agattcgttc agccagctgt cgaacggcgc tttcggtact gcgccattaa
     9601 gcatgtcgac aacctgacca tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     9661 agcggctgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     9721 tacgctcttg cgcaacatct tcaaaaattg gcgcgaagtt acggcagggg ccgcaccacg
     9781 gcgcccagaa gtcgatcacc acaggtagat catctttcag caatttgtcg agcgtttcac
     9841 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     9901 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     9961 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
    10021 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaatgggta
    10081 tgatagacac aggctttgga acgaaatcat ggcgaaccgc gcgcacttcg ggtaatctgc
    10141 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
    10201 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
    10261 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
    10321 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagagtc gccgtggcgc
    10381 acagtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
    10441 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
    10501 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcgat tgttcgcgcc
    10561 tggtttatcc agagtgtaac gccgcgtttt aaagaagcct tgcgctggat ggcagcaaac
    10621 cgcaaagcgt accatgtggt agatgaagcg gaactgacaa aagcgtcagg cacggaacat
    10681 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
    10741 agccaggcag gcgcgcagga ttgtgtactg gcgctggaaa acgaatctaa cccgcataac
    10801 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggcgttgt ggtgcaggat
    10861 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
    10921 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
    10981 accgtagtga ccacttccag cgagcagggg aaaccgctgt tcaaaaccag tctgccagcg
    11041 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
    11101 aacgatttgc gcgtgaagat tgatggtact ggcaacgttg ccgggctaaa tatctctgtt
    11161 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
    11221 gtggcaacgc tgccatccgg cgttttcctg caaatttact cactttctgc cagtaatact
    11281 ggcatccagt caatcggttt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
    11341 tggttgcagc caaagaaccc acgatgcgca gaaagcggtg atggatgcgg cgctttcagt
    11401 atgtgatggc gttgcttatc aataatcgcc cctttctttt gcgcatgtga gccccacaac
    11461 aaaaacacca cgccttcgcg gtgctggtta atcaggctga ttactttatc ggtgaaggtc
    11521 tcccagccga ggctggcgtg ggaatgtgcc tgacctgcgc gaaccgtcaa cacagtattg
    11581 agtagcagaa cgccctgacg cgcccagctt tcaagataac catgattagg gcgggtgaaa
    11641 ccgggaatag tattttccag ctctttatac atattcagca gtgacggcgg tgtcgcaatg
    11701 ccggggcgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
    11761 ccgagaatca ccactttaac gtcacccaac gctgtaaagc ggaacgcgtt aaagacatcc
    11821 ttttgtggcg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgtagggta
    11881 ttaagaaaat agggttgttg cttctcttca gccagcacgt catgccaggt taattcgttt
    11941 gccatctcgc tctcccgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
    12001 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
    12061 tcacacctgc ataagttgat gtaaaacaat aaa
//
LOCUS       DAZFRG010000144        12001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFRG010000144 DAZFRG010000000
VERSION     DAZFRG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.144"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..566
                     /gene="tssB"
                     /locus_tag="ACHKUC_003211"
     CDS             66..566
                     /gene="tssB"
                     /locus_tag="ACHKUC_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6619420.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            625..2163
                     /gene="tssC"
                     /locus_tag="ACHKUC_003212"
     CDS             625..2163
                     /gene="tssC"
                     /locus_tag="ACHKUC_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6619421.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2181..3518
                     /gene="tssK"
                     /locus_tag="ACHKUC_003213"
     CDS             2181..3518
                     /gene="tssK"
                     /locus_tag="ACHKUC_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6619422.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTPGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3515..4180
                     /gene="tssL"
                     /locus_tag="ACHKUC_003214"
     CDS             3515..4180
                     /gene="tssL"
                     /locus_tag="ACHKUC_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO6619423.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4193..5845
                     /locus_tag="ACHKUC_003215"
     CDS             4193..5845
                     /locus_tag="ACHKUC_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6619424.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDAAVLAGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGDEVQVATSTENAMPAQWIAQSGVQE
                     RSQRLCYLLKAESLMQWLNLNVLTALNGPEAKCPPLAMAVGLVTSLPAVDNNLWQVWI
                     TARTGLTTDIADTGTDATLPFPDALLRQLPRQSGFTPLRRACVTMLGITTAAGITMLC
                     LSATANRQLLRHIGDDLHQFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5903..6394
                     /locus_tag="ACHKUC_003216"
     CDS             5903..6394
                     /locus_tag="ACHKUC_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6619425.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6586..9219
                     /gene="tssH"
                     /locus_tag="ACHKUC_003217"
                     /gene_synonym="clpV"
     CDS             6586..9219
                     /gene="tssH"
                     /locus_tag="ACHKUC_003217"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6619426.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAASLCQTRAHAEITIEHWLLKL
                     LEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLIA
                     SLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPAR
                     QPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVFG
                     RDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRTL
                     DLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAANL
                     LKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKAR
                     YAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLTQ
                     LHARLAALDIEREAIEQDCVFCPEASPERLAELTDLRDELQAEAGHLEAQYQQEKALA
                     QQIMTLRQEGTDSTELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKDE
                     QSDLLSMEKSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKTE
                     TALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRPY
                     SVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQLL
                     EEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVARR
                     LRQHYGLETEISNSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHHK
                     PARLRLDWDDEDGIVLEFDEK"
     gene            9231..11741
                     /locus_tag="ACHKUC_003218"
     CDS             9231..11741
                     /locus_tag="ACHKUC_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6619427.1"
                     /translation="MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMAD
                     IISELNGLSDKIQKLSDDATTANADPADMAAQIALITSRINDLTAPVILMHAPKGVAV
                     ASGEHLQLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIESGEYNIQFNFKDDDGIPYANTRYIAFMADGTRKE
                     GITDENGKTDNFNCDKEQEIEVRLLHQSIDMVEGGVNE"
     gene            11734..>12001
                     /locus_tag="ACHKUC_003219"
     CDS             11734..>12001
                     /locus_tag="ACHKUC_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619428.1"
                     /translation="MSDDRGSSTGTAEKKEECVKEFIVSDKFKKMMDDAFNATKSVLK
                     KRAKNLKDWTENDKQEFSQIFGVSGDVIITSTYFAKRVADKLSEN"
BASE COUNT         2802 a         3244 c         3525 g         2430 t
ORIGIN      
        1 cgataatccc ctgaatataa ttatgtcaat aaccatcaga aaaagtggat gatgaggaaa
       61 aggatatggc cgacagtttc cagaatgaag ttcccgccgc tcgtgtaaat atcaagcttg
      121 atctgcatac aggcaatgca aaaaagaaag ttgaactccc cctcaagctt cttgccgtag
      181 gcgattacag taacggaaaa gagcagcgtc cgctgtccga acgggacaag attgatatca
      241 ataaaaacaa cttcaacagc gtcatggctg agttttcgcc tgcggttaat ttaacagtag
      301 aagatacgct aagcggaaac ggtaatgaac aaaatatcgc gcttgagttt aaaagcctga
      361 aggattttga acccgaacag gtagcaaaaa atattccgca gcttcgtgta ttactcgcta
      421 tgcgcaattt actacgtgac ctgaaatcca atctgctgga taacgcaacg tttcgccgtg
      481 aactggaaaa tatcctgaaa gacccgacgc tcagttcaga actgcgtgat gaactggcga
      541 aaattgcgcc gcaggagaac gtttaacccg aacgcgtatt caggttaaac cggagaagaa
      601 aggatcactg aaaaggaaat gctgatgtct gtacaacaag aacatgccac ctctgaaact
      661 gcaacactca ccaccactga gtccggcggc gtttatcagt ccctgttcga taaaattaat
      721 ttaaccccgg tctcttccat tcaggaaatc gatttatggc aaaacagcga aacgctggcc
      781 gatgcctcac ccgatgagcg cgtgacggcg gcgattcacg ttctgctttc ctgtctggcg
      841 aaatcaggcg aggacgtggt taagctcgac aagagcctgc tggattttca tatcgacgat
      901 ctggatcaga aaatcagtaa acagcttgat gcggtcatgc accaccctga attccagaaa
      961 gtcgagtcgc tgtggcgtgg tacatggttc gtcgtacagc gcactgattt tcgcaaaaat
     1021 gtcagaattg aattgctgga tatcagtaaa gaacatctgc ggcaggactt tgatgattct
     1081 ccggaaatca ttcagagtgg tttatatcgc catacataca ttcaggaata tgatacgccg
     1141 ggtggcgaac ctgtagcctc attaatatcc agctatgaat ttgataacag cccgcaggat
     1201 attgccctgc tgcgtaatat ttccagagtg tctgccgctt cccatatgcc ttttatcggt
     1261 tctgtcggac cgaaattctt ccttaaaaat tcgatggaag aagtcgccgc gattaaagat
     1321 atcggcaact actttgaccg cgcagaatat attaaatgga agtcgttccg cgatacggat
     1381 gacagccgct atgtgggatt agtgatgccg cgcgtgctgg gccgtctgcc ctatgggccg
     1441 gacacggtgc cggtacgcag ctttaactat gtggaagaag tcaaaggccc ggatcacgaa
     1501 aagtacctgt ggacaaacgc ctcgttcgcc tttgccgcca atatggtgaa gagctttgtg
     1561 aataatggct ggtgtgtgca gatccgtggt ccacaggcag gtggcgcagt ggccgatctg
     1621 cccatccatc tttacgatct cggcaccggc aatcaggtca aaattccgtc cgaagtgatg
     1681 atcccggaaa cccgcgaatt tgaatttgcc aaccttggct ttattccgct ctcttattat
     1741 aagaatcgcg attacgcctg cttcttctcg gcaaactccg cccagaaacc ggcgttgtat
     1801 gataccgctg acgccaccgc caacagccgt attaatgccc gtttgcccta tattttcctg
     1861 ctgtcccgca ttgcgcatta cctgaaaatc atccagcgcg agaatatcgg taccaccaaa
     1921 gaccgccgcg tactggaact ggaactgaac acctggatcc gcacactggt gacggagatg
     1981 accgatccgg gtgatgaact gcaggcgtct cacccgctgc gcgacggcaa ggttatcgtg
     2041 gaagatattg aggacaatcc gggcttcttc cgcgtcagac tctttgccgt gccgcatttc
     2101 cagattgaag ggatggatat caacctgtca ctggtttccc agatgccgaa agcgaaagcc
     2161 tgaccgggcg gtgatgtgat atgaaaattc atcgaccact gtgggctgag gggacattcc
     2221 tgtcctctca gcagtttcag cagcaggccc gctgggaagc gttcagcaat gacagcattg
     2281 cgcaactttg tattcgtcat ccgtggggga tcgcaaacgt gctctttgac cgggatgccc
     2341 tgacgcccgg aaagctgaaa acgcaggcag tacgcctgcg ttttgctgat ggtacgctga
     2401 tcgattcaga cgtggcggat attctgcctc ctgcctgtga cctgcgcacg ctggagggcg
     2461 gcagcgcgat tgttttgctg gcgctgccgc ttgcgcatgg taacggcggt aatctcggtc
     2521 agggcgagca ggctgaaagg ccgctgcgct atcgtcagga atggcaaaag gtgcaggata
     2581 tctacggcag tgacagtgaa gatatggccg ttgagcgcca tgcactgtcc ctgcgctttg
     2641 cccatgataa caatcaggat tacatcacct gtccgctggc gcgtctggtg cgggatgttc
     2701 agggcaactg gacgcaggat gagggctata ttccgccgtt actggcgttt aacgcccatg
     2761 acgggctggt acagcgtctg gacacactgc tgttgcagct acgggcgaag tgccagcgtc
     2821 tgatggcgat gcgccgggaa agcaaccagc gtatggcgga ctttgccgtc gccgatgtct
     2881 ccctgttctg gctgcttaac gcgctgaact ccgccgagcc ggtactgagt gatttcctgc
     2941 gctacccggc ggttcatccc gaactggtct ggcgggagct ggcgaggctt gccggtgcgc
     3001 tgctgacgtt ttcccttgaa cataacgtca gcgccgttcc gccctatgtc cacgaatcgc
     3061 cgtccatcgt ctttccgcca cttttctccc tgctcagcga actgctggaa gccagcctgc
     3121 cgtcacgggt gatcgcgctg gatctggcgt ccctgccggg caaccgctgg aaagcagacc
     3181 tgcacgatcc gcgcctgcgg gaagaggccg acttttacct gtccgttcgt tcctctctgc
     3241 ctgcgcatca ggtactgcac cagctaccgc tggtctgcaa aatcggtgcg cctgatgacg
     3301 tcacgctgct tatcaatgtg gcgctgaatg gtgttccact ggtgccgctt acctcagttc
     3361 cggcagcgct gccactgcgg ctggagaacc agtattttgc gctggatatg cacagtgacg
     3421 cggctaaatc gatgctggag tccgggtgct gcatgattta tgcgccggga accatgggcg
     3481 acctgaaacc tgaactgttt gcggtgctgc gcacatgaaa aaagagacgg atatcaatat
     3541 cgacgcgctg ctgcgcgata cgtttctgac ggtggtggaa ctgcgtcagg gaaccaccgt
     3601 gcgtcacggt atggagctgt atcgccactg ccagcggcag gtggaactgg tccgcgaacg
     3661 cctgaaagat gccgggttca gccgggagag cgtggagcac attacttacg cgcagtgcgc
     3721 cctgctggac gaaacggtcc tgagccgtgg cggcatggac gacggtcagg ccatctggat
     3781 gaaggatcca ctccagtcgc atttcttcaa cactctgcag gcaggcgagc tgctgtacga
     3841 acggatgaag caggtgttgc aggagcccgc ccccgcgcag gcagtgctga cctgctttca
     3901 ccgtgtcctg ctgctgggat ttcgcgggcg ttatcaggat ccggtggccc cggagcgtga
     3961 ccagcttatt tcgacgctca acgggctggt cgcgccattt ggcgtgctgc cggaaacggc
     4021 ggtactgaac gtgccgttat ccccccgtca gcatcctttg ctgcattcgc cgtttttctg
     4081 gctggtaacg ctgacgctgc tactggccgg tgtctggtgg ggactgcacc actggctgaa
     4141 cgtgctggtg gatgaactgc tgccgcaaag cctgcgttaa agggagccgt gagtgaggaa
     4201 cacgctgaaa caggccatcg tgctgtgggg aatggtgtta ctgctggtgc tgtggtgggt
     4261 gtttgtcagt ccgtctggcg tactgagatg ggccggtgca gcagttatcg ttctggcggt
     4321 tgccgcattg ttgatttacc ggcgcaggca gacgtggacg gagatgaccg gcgatgccgg
     4381 gttgttatcg ctgccgccgg aaacgtaccg acaaccggta gtgctggtct gtggcgatat
     4441 gtcggcgcat ctgttcactg acagcccggt ccggcaggtt tcagaagggc tgtatctgca
     4501 tgtttctgat gaagaacagc ttgtggcgca ggtggagcga ttactgaccc ttcgcccggc
     4561 gtgggcatcg cagcttgccg tggcgtatac cgtcatgccc ggcatgtacc gggatgcggc
     4621 ggttctggcc ggacaactgc gccggttcgc ccacagtatg gcgacagtgc gtcgccgggc
     4681 gggcgtaaac gtcccctggc ttctctggag cgggctgtcc ggctcgccct tgccggagag
     4741 agcgaattca ccgtggttta tctgtaccgg agacgaagtt caggtagcaa catccacaga
     4801 gaacgcgatg cccgcgcagt ggattgcaca atccggcgta caggagcgca gtcagcgact
     4861 ctgttacctg ctgaaagctg aaagcctgat gcagtggctg aatcttaatg tgctgacggc
     4921 actgaacggc ccggaggcga aatgcccgcc actggcgatg gccgtggggc tggtcacctc
     4981 gttgcctgcg gtggataaca acctgtggca agtgtggatc accgccagaa ccggcctgac
     5041 gacggatatc gcggacaccg gcacagacgc cacgctgccg tttccggatg ccctgttacg
     5101 gcagttgccg cgtcagtcgg gctttacccc gctgcggcgg gcctgcgtga ccatgctggg
     5161 cattaccacc gcggcgggta tcaccatgtt gtgcctgtca gccacggcaa atcgccagtt
     5221 attacggcat atcggtgacg atctgcacca gttttatgcc gttccggcgg aggaatttat
     5281 caccaaagcc cgtcacctgt cggtgctgaa agacgatgcg gtcatgctcg atgggtatta
     5341 ccgggaagga gaaccgctgc gtctcggtct ggggttatac cccggtgaac aaatccgcca
     5401 gccggtatta cgcgccattc gcgactggcg tccgcctgag caaaaaatgg aagtgacagc
     5461 ctcgcttcag gcgcagaccg tgcgtcttga cagtatgtcg ctgtttgacg tcggacaggc
     5521 ccgcctgaaa gacggctcga caaaagtgct ggtggacgca ctggtgaaca tccgggcaaa
     5581 accgggctgg ctgatcctcg tggccggata taccgacgcc accggcgacg aaaaaagcaa
     5641 tcagcagtta tcgctgcgac gtgccgaagc ggtgcgtaac tggatgctgc agaccagcga
     5701 catcccggcc acctgttttg ccgtacaggg actgggcgag agccagcctg cggcgaccaa
     5761 cgacacgcca cagggccggg cagtcaaccg gcgtgtcgaa atcagtcttg ttccgcgttc
     5821 tgacgcctgt caggacgtga aataaaacat accgccggaa gaaggcggtg cttcaatcac
     5881 actaacaagg agagtaattc tcatggctat tcctgcttat ctctggctga aagatgacgg
     5941 cggcgcggat atcaaaggtt ccgtggacgt tcaggggcgc gaaggtagca tcgaagtggt
     6001 ggcgctggat cacgatgtgt acatcccgac cgacaacaac accggcaaac tgaccggtac
     6061 ccgtactcac aagcccttta cgtttaccaa agaaatcgat gcctccagcc cgtatctcta
     6121 caaagctgtg accaccggac agaccctgaa aacggcagaa tttaagtttt accgcatcaa
     6181 cgatgccggt caggaagtgg agtacttcaa catcacgctt gataacgtca agctggtcag
     6241 agtcgctccg cttatgcacg acatcaagga tccttccaga gagaagcata accacctgga
     6301 acgtattgag ttccgctacg agaaaatcac ctggacttac aaagacggca acatcattca
     6361 ttccgactcg tggaatgagc gtccttccgc ctgataactg cggcagacag gaaactgtct
     6421 gcctttctgt tgtgtgcttt gtccagtgcc gcaggcaccg gtactgagca aagcataccc
     6481 acaatctggt gaaacctgat gggcctgagt gaggtaacgg ctatgggcgg tagcgtgcct
     6541 gaacgcggat cgcaggaata cagaacacga taaggaaacc cttctatgac aggaaatcac
     6601 cccgccgcgc tgctgcgtcg ccttaaccca tactgtgcac gggcgctgga cgccgcctcg
     6661 ctgtgtcaga cccgcgccca tgcggaaata accattgaac actggctgct gaaactgctg
     6721 gagcagggag aaggcgatat cacggtgatt gcccgccgct atgaatggga tatcgacacg
     6781 ctctggcagt ctctgctggc acatctggac accttacccc gcccggtccg cgaacgtcct
     6841 caactttctg aaccgctggc agcgcttatc cgacaggcgt ggctgatagc gtcactggaa
     6901 ggcgacgatc cacaaatccg cagccagcat ctgctgatgg cgctgacaga aaaaccgatg
     6961 ctgcccgcct gtaatgacct gtgggtattg ctgagtctga gccgcgtgca gcttgagcgg
     7021 ctgcgtcccc tgctggatgc gcagtcggat gaatgtccgg cacgtcagcc acaggtcacc
     7081 gaaccgctga cctctgcact gccggagacg gcaacggcgg acgcaccggc aaaaacgctg
     7141 acggagaaac aggatgacgc cctgctggcg gtgcttaacc gctttaccga agacgtgacg
     7201 gaaaaagccc gcagcgggcg aatcgacccg gtattcgggc gcgacacgga aattcgccag
     7261 atggtcgata tcctctcccg tcgccgcaaa aacaacccga ttctggtggg agaaccgggt
     7321 gttggcaaaa ccgcgctggt ggaagggctg gcgctgcgta tcgccgaagg caacgtgccg
     7381 gacagcctga aaacggtgca tatccgcacg ctggatttgg ggctgttgca ggccggggcg
     7441 ggtgttaaag gtgaatttga acagcggctg aaaaacgtga ttgacgcggt gcagaaatcg
     7501 ccggagccgg tgctgctgtt tattgatgaa gcccatacca ttatcggcgc ggggaatcag
     7561 gcaggcggcg cggatgccgc caacctgctg aaaccggcgc tggcaagggg cgaactgcgc
     7621 accatcgccg ccacaacgtg gagcgaatac aaacagtatt ttgagcgcga cgccgcgctg
     7681 gagcgccgct tccagatggt gaaagtggac gagccggatg atgacaccgc ctgcctgatg
     7741 ctcagggggc tgaaagcccg ctatgcgcag caccacggcg tacatatgct ggacagcgcc
     7801 attcagaccg ccgtgcgcct gtcgcgccgc tatctgaccg gacgccagtt gccggacaag
     7861 gcggttgatt tgctggatac cgccggagca agagtccgca tgagcctcga caccctgccg
     7921 gaaccgttga cgcagcttca tgcgcgactg gcggctctgg atattgagcg ggaagcgatt
     7981 gagcaggact gcgtgttttg tcccgaagcc agcccggagc ggctggcgga actgaccgat
     8041 ttgcgtgatg agctacaggc agaagccggg catctggaag cgcagtatca gcaggaaaag
     8101 gcactggcgc agcagattat gacgttgcgt caggaaggga cagacagcac tgaactgcaa
     8161 cagcaactgc gaacgcatca gggctttgca ccgctgctgg cgctggatgt ggacgcccgc
     8221 gccgtcgcca cggtggtggc ggactggacc ggcatcccgc tctcttccct gctcaaagac
     8281 gagcagagcg acctgctcag tatggaaaag agccttgaaa accgcgtggt cgggcaaagc
     8341 ccggcgctct gcgccatcgc acagcggctg cgggcggcta aaaccggcct cacgccggag
     8401 aacggcccac agggggtatt cctgctgacc ggccccagcg gcaccggtaa aaccgaaact
     8461 gcgctcacac tggccgacac cctgtttggc ggtgaaaaat cccttatcac cattaatctt
     8521 tcggaatatc aggaaccgca taccgtttcc cagttgaaag gttcgcctcc gggctatgtc
     8581 ggctacggtc agggcggcgt gctgaccgaa gcggtacgca aacgccctta cagcgtggtg
     8641 ctgctcgacg aagtggaaaa ggcgcatcgc gacgtgatga acctgttcta tcaggtgttc
     8701 gaccggggct ttatgcgcga cggcgaaggg cgggaaatcg acttccgtaa caccgtgatt
     8761 ctgatgaccg ccaatctggg cagcgaccac atcatgcagc tgctggagga aaaaccggac
     8821 gccacggacg cagacctgca tgaactgctg taccccctgc tgcgagacca ttttcagcct
     8881 gcactgatgg cgcgctttca gacggtgatt taccgcccgc tgggacagga ggcgatgcgc
     8941 gccattgtgg aaatgaaact ggcgcaggtg gcccgccgtc ttcgccagca ctatgggctg
     9001 gaaacggaaa tcagtaacag cctgtacgac gccctgaccg ccgcctgcct gctgccggac
     9061 accggtgcgc gtaatatcga cagcctgctg aaccagcaaa tcctgccggt cttaagccag
     9121 cagttgctgg cgcagcaggc cgtgcatcat aagcctgccc gactgcggct tgactgggat
     9181 gatgaagacg ggattgtgct ggaatttgat gagaaataat ggaggctctt atgaacgtac
     9241 agttcttcga ccacgcacac cacaagctca aaattcgcgg cctgcaaagt ccggtggatg
     9301 tgctgacctt tgaaggccgc gagcaactga gtaccccgtt ccgctacgac attcagttta
     9361 ccagcagcga taaagccatc gcgccggagt cggtgctgat gcaggatggc gcattcagcc
     9421 tgacggcacc gccggtacag ggaatgccgg tacagactgc cctgcgcacc ctgcacggcg
     9481 tcattaccgg ctttaaacat ctctcatcgt cacaggacga agcccgttac gaagtgcgcc
     9541 ttgaaccccg tatggcgctg cttacccgca gccgccagaa cgccatctac cagaaccaga
     9601 cggtgccgca aatcgtcgaa aaaatcctgc gcgagcggca ccagatgcgc ggtcaggact
     9661 tcgtgtttaa cctcaaaagc gaatacccgg cccgcgaaca ggtgatgcag tacggtgagg
     9721 acgacctgac gtttgtttca cgcctgctgt cagaggtggg tatctggttc cgctttgcca
     9781 ccgacgcccg gttgaaaatc gaagttatcg aattttacga cgaccagagc ggctacgagc
     9841 gcgggctgac gctgccgctt cgccatcctt ctgggctgtt tgatggtgaa acggaagccg
     9901 tatgggggct gaacaccgcg tacagcgtgg tggaaaaaag tgtctccacg cgggactaca
     9961 actaccgtga agccacggcg gaaatgacca caggccagca tgacgccacc gggggcgata
    10021 acaccactta cggcgaagcc taccactatg ccgacaactt cctgcaacaa ggcgataaag
    10081 aggcagcgga aagcggcgcg ttttatgccc gtatccgtca cgaacgctac ctgaatgaac
    10141 aggccattct caaaggccag agcaccagtt cactgctgat gccggggctg gaaatcaggg
    10201 ttcagggcga cgacgcgcca gcggtgttcc gcaagggcgt gctgattacc ggcgtgacga
    10261 ccagcgcggc gcgtgaccgc agctatgagc tgacgttcac ggcgataccg tactcagagc
    10321 gctacggcta ccgtccggcg cttatccccc gccccgtaat ggccggaacg ctcccggcgc
    10381 gggtcaccag tacggtgaag aacgacatct acgcccatat cgacaaggac gggcgctacc
    10441 gggtcaacct cgactttgac cgcgatacgt ggaaaccggg ctatgagagc ctgtgggtgc
    10501 gccagtcgcg cccgtatgcg ggagacacct acggcctgca cctgccgctg ctggcaggaa
    10561 cggaagtcag tatcgccttt gaggaaggca acccggaccg accttatatt gcaggcgtaa
    10621 aacacgactc ggcacacact gaccatgtga ccattcagaa ctacaagcgc aacgtactgc
    10681 gtaccccggc gaacaacaaa atccgcctcg acgatgagcg cgggaaagag catatcaagg
    10741 tcagcaccga atatggcggc aagagccagt taaacctcgg ccatctggtg gatgccggga
    10801 aacagcagcg cggggaaggt tttgaactgc gtaccgatct gtggggcgcg gtgcgggcga
    10861 agaaaggcat ttttatcagc gccgatgcgc aggacaaggc gcagggtcag gtcagagaga
    10921 tggccgacat tatcagcgaa ctgaacggtc tgagcgataa aatccagaaa ctgtccgatg
    10981 acgcgacgac ggcgaacgcc gacccggcgg atatggccgc gcagatagca ttgataacca
    11041 gccggataaa tgacctgacc gccccggtga ttttgatgca cgcaccgaaa ggcgtggcgg
    11101 tcgccagcgg cgagcacctg caactggccg cagtgaaaaa cctccagata aatgcgggaa
    11161 acaacgccga tatcggcgtg gtgaagaata tgtttattgg cgtggggcgg gcgctgagcg
    11221 tgtttgtgcg caaggcgggt atcaaactgt ttgccaataa aggggctgtc tcggtacagg
    11281 cacagaacga tctgatggaa ctgttggcac agaaatcgat agagatcacc agtacggaag
    11341 atgagatcaa gatcaccgcg aagaagaaaa tcacccttaa cggcggtggc agctacatcc
    11401 ggctggatgc ctgcgggata gaagcgggaa cgccgggaga atataacgtt aaggccggat
    11461 attacgggcg taaaccaaag gcgaagctga cgccggagct gatggcgttt ccggtgattg
    11521 agagcgggga atataatatt cagttcaatt tcaaggatga tgatgggata ccttatgcaa
    11581 atacgagata tattgcgttt atggccgatg gaacaaggaa agaaggtatt accgatgaga
    11641 atggaaagac tgataatttt aactgtgata aagagcagga aatagaggtc aggttactac
    11701 atcagagcat tgatatggtt gagggtggtg taaatgagtg atgatcgtgg tagttcaaca
    11761 ggtacggcag aaaagaaaga agaatgtgtt aaagagttta ttgtttccga taagtttaaa
    11821 aaaatgatgg acgatgcctt taatgcaact aaaagtgtgt taaagaaaag ggctaagaat
    11881 ctgaaggatt ggactgaaaa cgataaacag gaatttagtc aaatatttgg tgtttctggt
    11941 gatgtaatta ttaccagtac atattttgcg aagagagtag cagataaatt atcagaaaac
    12001 g
//
LOCUS       DAZFRG010000145        11962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFRG010000145 DAZFRG010000000
VERSION     DAZFRG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.145"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1335)
                     /gene="uidB"
                     /locus_tag="ACHKUC_003220"
     CDS             complement(<1..1335)
                     /gene="uidB"
                     /locus_tag="ACHKUC_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO6619429.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRK"
     gene            complement(1332..3143)
                     /gene="uidA"
                     /locus_tag="ACHKUC_003221"
     CDS             complement(1332..3143)
                     /gene="uidA"
                     /locus_tag="ACHKUC_003221"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO6619430.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNHVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPLGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3533..4123)
                     /gene="uidR"
                     /locus_tag="ACHKUC_003222"
     CDS             complement(3533..4123)
                     /gene="uidR"
                     /locus_tag="ACHKUC_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO6619431.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4352..5119)
                     /gene="hdhA"
                     /locus_tag="ACHKUC_003223"
     CDS             complement(4352..5119)
                     /gene="hdhA"
                     /locus_tag="ACHKUC_003223"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO6619432.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5231..6259)
                     /gene="malI"
                     /locus_tag="ACHKUC_003224"
     CDS             complement(5231..6259)
                     /gene="malI"
                     /locus_tag="ACHKUC_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO6619433.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6434..8026
                     /gene="malX"
                     /locus_tag="ACHKUC_003225"
     CDS             6434..8026
                     /gene="malX"
                     /locus_tag="ACHKUC_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO6619434.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8036..9208
                     /gene="malY"
                     /locus_tag="ACHKUC_003226"
     CDS             8036..9208
                     /gene="malY"
                     /locus_tag="ACHKUC_003226"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO6619435.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQPDGWFCDMGKLEAVLAK
                     PECKILLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFIRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            9312..10313
                     /gene="add"
                     /locus_tag="ACHKUC_003227"
     CDS             9312..10313
                     /gene="add"
                     /locus_tag="ACHKUC_003227"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO6619436.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10349..11389)
                     /gene="ydgJ"
                     /locus_tag="ACHKUC_003228"
     CDS             complement(10349..11389)
                     /gene="ydgJ"
                     /locus_tag="ACHKUC_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6619437.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEIAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11632..11757
                     /gene="blr"
                     /locus_tag="ACHKUC_003229"
     CDS             11632..11757
                     /gene="blr"
                     /locus_tag="ACHKUC_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO6619438.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         2905 a         3139 c         2913 g         3005 t
ORIGIN      
        1 tttacgatta tcaatttcaa caacgatttc tttgaatttt ttatccgtga gtggataaaa
       61 ccagataatg acgaatgcca gtagcataaa tccgcaaggt actaaggcaa ttgatgtgcg
      121 gatgcccata ataacttccg gcgtttgcgc ctgattggcg atatatccgc ttaatcccaa
      181 aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa atgagaatag
      241 tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt ctacggtatc
      301 agcttccagt gcccacatca cggtcatggt aacgccctgg ccaattgatg cgatggccaa
      361 cgcaaccaac gccaccggca gtgaccagac ggaaacccag aagaacagca gataaccgca
      421 ggttcccagc aaagcgccaa tcaggaaggt attcttttta ccgatcctcg cgaccatccc
      481 cggcaccagc ggtgccgatg ccacagtacc gaccaggttt tgcaccagta ccagcacagt
      541 gaacagcccg gtatcattta acacatagcg cacgtagaac aacgacgagg cgctgaccgc
      601 gaaggtcgaa atcagcacac acagcgcacc gatgcacaac ataaacagcg ggcgattccg
      661 tttcagggtt tgcagactga tcttcaatga cggctgcgcc acgatacgta ccacattctc
      721 acgcgtcgat ttgaagcaga tgaagtaaag caccattccg gcaatcgcca gcacaattgt
      781 ccagaaatga tataccgaca ccatctcttc cgggctggag ttcttaatgc tcggtcctat
      841 cagaaatgcc aggcagacaa aggtcaatga agcggcaatc ccgcgagccg cgcccagacg
      901 ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaagt gaaccataag gaatattcac
      961 caggctgtag caaagcccga gtcccatgta ggtcaaatat gcatacacaa ctttgctgct
     1021 atggctccag tcggtcggca cccagaatac cagcacgctg aagatcatta acggcgcagt
     1081 accgaagagt aaaaacgggc ggaattttcc ccatcgggta ttcacactgt ccaccactcg
     1141 tccggcaaag acatcggcga aggcatcgaa tacccgcacc agtaacagca tggtgcccgc
     1201 cgcagcggca ccgacgccag cgacgtcggt gtagtaactc aacaggaaga gcgcccccat
     1261 tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag ccgacgatgg cgcgccagga
     1321 gagttgttga ttcattgttt gcctccctgc tgcggttttt caccgaagtt catgccggtc
     1381 cagcgttttt gcagcagaaa agccgcagac ttcggtttgc ggtcgcgagt gaagatccct
     1441 ttcttgttac cgccaacgcg caatatgcct tgcgaggtcg caaaatcggc gaaattccat
     1501 acctgctcac cgacgacagc gctgacgcga tcaaagacgc ggtgatacat atccagccat
     1561 gcacactgat actcttcact ccacatgtcg gtgtacattg agtgcaggcc ggctaacgta
     1621 tccacaccgt attcggtgat gataatcggc tgatgcagtt tctcctgcca ggccagaagt
     1681 tctttttcca gaaccttctc tgccgtttcc aaatcgccgc tttggacata ccaaccgtaa
     1741 taacggttca ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg agcgtcgcag
     1801 aacattacat tgacgcaggt gatcggacgc gtcgggtcga gtttacgcgt tgcttccgcc
     1861 agtggcgcga aatattcccg tgcaccttgc ggacgggtat ccggttcgtt ggcaatactc
     1921 cacatcacca cgcttgggtg gtttttgtca cgcgcaatca gctctttaat cgcctgtaag
     1981 tgcgcctgct gggtttcccc gttgactgcc tcttcgctgt acagttcttt cggcttgttg
     2041 cccgcttcga aaccaatgcc taaagagagg ttaaagccga cagctgcagt ttcatcaatc
     2101 accacgatgc catgttcatc tgcccagtcg agcatctctt cagcgtaagg gtaatgcgag
     2161 gtacggtagg agttggcccc aatccagtcc attaatgcgt gatcgtgcac catcagcacg
     2221 ttatcgaatc ctttgccgcg caaatccgca tcttcatgac gaccaaagcc agtaaagtag
     2281 aacggtttgt ggttgatcag gaactgttcg cccttcactg ccactgaccg gataccgacg
     2341 cgcagcaggt agatatcaca ctctgtctgg cttttggctg tgacgcacag ttcatagaga
     2401 taaccttcac ccggttgcca gaggtgcgga ttcactactt gcaaagtccc gctggtgcct
     2461 tgtccagttg caaccacctg ttgatccgca tcacgcagtt caacgctgac atcaccattt
     2521 gccaccacct gccagtcaac agatgcgtgg ttacagtctt gcgcgacatg cgtcaccacg
     2581 gtgatatcgt ccacccaggt attcggcgtg gtgtagagca ttacgctgcg atggatcccg
     2641 gcgtagttaa agaaatcatg aaagtaagac tgctttttct tgccgttttc gtcggtaatc
     2701 accattccca gcgggatagt ctgccagttc agttcgttgt tcacacaaac agtgatacgt
     2761 acacttttcc cggcaataac atacggagtg acatcggctt caaatggcgt atagccgccc
     2821 tgatgctcca tcacttcctg attatttacc cacactttgc cgtaatgagt gaccgcatcg
     2881 aaacgcagta caatacgctg gcccgcccaa cctttcggta taaagacttc gcgctgatac
     2941 cagacgttgc ccacatgatt acgaatatct gcatcggcga actgatcgtt aaaactgcct
     3001 ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc accaacgctg atcaattcca
     3061 cagttttcgc gatccagact gaatgcccac aggccgtcga gttttttgat ttcacgggtt
     3121 ggggtttcta caggacgtaa cataagggac tcctcattaa gataaaaata ctggtcaacc
     3181 tttaatctga ttagttaacc aattctgtgt tgcacacagt tatctgtgag aaacccatgg
     3241 aagagaaatg gaaataaaaa gaactgacac cgcgatcaaa aaaccaaaaa ctgcaatacc
     3301 cacagcgtga tattgatcgc attaatcttt aaaacagcgt tgacagaagc cacaaaataa
     3361 gcaatcgaac gttcgttcga acatattggg aacgataaac cctcatgata tattgctacg
     3421 atgaatattt aggtgggatt ctccgatccc tgggaggatg atttttgcac agagaaatta
     3481 cgatatgaaa cgcgggctca gttgtcacca gcccgcggaa agagagagag gatcaggatg
     3541 cggttaagat accgccaatc atcgcgcgta aaccctgagc aaacgacgct tcacgagcca
     3601 ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag atccagcagt aaacgtgaag
     3661 tcattgccgt gttgatgtct gggcttatct cgcctttttg ctgggcatca cgcatccgct
     3721 gggcaacaaa ttccgtgatc gtcatatgct tatttttaag catggcggcg acctgtgggt
     3781 tacgcatccc ttccgccata atttcgacca ccagcgctcg ttgcccaaag gcttcatggg
     3841 tgagagagac aatagactcg accatatagt caacgaaatg aatcccttca atcggttccc
     3901 ggaaacgggc cagcgccctc tcctggtcct gtaagataat cgcctgaatc aaggcttctt
     3961 tggagatgaa atggtgatag agcgtcccgg gactaatggc gcaggattta cagatggctt
     4021 tcatcgaggc actgtgaaat ccattttctg aaaaaatctc tctggcagca ttgaggatcc
     4081 gggtccgtgt cggttgtgct tcagtctgca tgttatccat cattaaacca ttatagttag
     4141 catgaataac tagtttaccg cgttgtcagg tgagcgccta tcattgctgt tgaatagtgc
     4201 gaaggcacac tcaattcgta agctttttat ccagatattg gtcaaccaat agccgtgatt
     4261 tggctgccag ttgtttagca gagacgttga tgagttgcac acgctatccc ggtaaacaac
     4321 acatacggct gtttaccggt tcgttagtgt attaattgag ctcctgtacc ccaccaccgg
     4381 agacggtgag aatttgtccg cttacccagc tggctgcagg cgagcaaagg aacagcgccg
     4441 cgttagctat atcttgcggt tggcccagac gtctgattgg tgtgtgttgc aacattttct
     4501 gttcaatctc tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa
     4561 tgccattcac ccgaatattt ttttcaccca ggtcaaacgc catatttctg accagatgac
     4621 tggccgcagc tttagatgat gcataggagg tcatgtttat atttttattt tctgccgcca
     4681 tagaagtaat ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt
     4741 gtgacagatg gaaaaaagaa aatacattca gttcataagc gcggcgaaaa tctgccattg
     4801 gcatatcaaa cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac
     4861 ccaacttact gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcgc
     4921 agcggcaggc aaatgcctga ccacccagtt gttgaatttc atctacaaca tgattagctg
     4981 catcggcgtt aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga
     5041 tttctttacc aatacctgca cccgcacctg taatgatggc gcattttccg tcgagtctca
     5101 ggttgtcaga attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc
     5161 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
     5221 aaagagagga ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
     5281 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
     5341 ctggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggcgtt gcatcggtaa
     5401 atgccgccag cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
     5461 ttcctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
     5521 cggcgctgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
     5581 ggctggaagt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
     5641 tcgcacaata gccccccacc ctttctgccc gggttaatga ggaactttgc cctcccagcc
     5701 aggcgatacg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
     5761 tgttgtccgg gcgaaccgta tcaacgtcat caagataact ggcacgggaa gcgaaaatca
     5821 ccgggatagc tttttcttct gctatccgtc gcaggtcatc gctgcttcct gcagccccgg
     5881 caatgaccac accatcgaca ccctgattca gtaatagtga aaaccgctgt gccagctgct
     5941 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
     6001 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
     6061 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
     6121 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     6181 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     6241 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     6301 ttacctttta tttatcttat acccgctatt atcgttgtgt aatgtgattt atgcctcact
     6361 aaaatttgat aaaacgtttt atcttctcgc gcaatttatt gaatccagat tgttctctac
     6421 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     6481 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     6541 cggcattggt agttctctta gcagccatga tgtcatcacc ctgatcccgg tcctgggcaa
     6601 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     6661 cttgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     6721 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     6781 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     6841 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     6901 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgctggcatt
     6961 cttcggcggt acgcgcttcg taccaatcat ctcctcgcta gtgatgggtc ttgtcggcct
     7021 ggtgattcca ttagtctggc cgattttcgc catgggtatt agcggcttag gccatatgat
     7081 caacagcgcg ggagatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     7141 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     7201 ggaagtctgc ggtcaaaccg tcagcggtgc actgaccatc ttccaggcgc aattgagttg
     7261 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     7321 tgcgtttctc ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga
     7381 aaatcgccat aaaattaaag gtctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     7441 cacgaccgaa ccgctggaat tccttttcct gttcgtagcg ccagttctgt atgtcatcca
     7501 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     7561 taccgacggc aatatcatcg actttgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     7621 gtggtacatg gtgccagtag tggcggcaat ctggtttgtc gtttactaca tcatcttccg
     7681 tttcgctatc acccgcttca atctcaaaac cccggggcgc gatagtgaag ttgccagttc
     7741 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     7801 cgaagcctta ggcggtgccg acaatattgt cagcctcgat aactgcatta cgcgtctgcg
     7861 tttgtctgta aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     7921 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     7981 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     8041 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acgcagtggg attatgtcgc
     8101 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     8161 tgccccctgc attatcgagg cgctgaatca gcgcctgata cacggcgtgt ttggctacag
     8221 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     8281 caccgccatc gatcctcagt cggtggtgta tggcccttct gttatctata tggtttcaga
     8341 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacac ccgcctatga
     8401 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     8461 gcagcctgac ggctggtttt gcgatatggg caagctggaa gccgtgttgg cgaaaccaga
     8521 atgcaaaatt ctgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     8581 tgagctggag atcatggctg acctgtgcga gcgtcatggt gtgcgggtta tttccgatga
     8641 aatccatatg gatatggttt ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     8701 cggagactgg gcgttgctaa cgtcgggatc aaaaagtttc aatattcccg ccctgaccgg
     8761 tgcttacggg attatagaaa atagcagtag ccgcgatgcc tatttatcgg cactgaaagg
     8821 acgtgatggg ctttcttccc cttcggtact ggcgttaact gctcatatcg ccgcctatca
     8881 gcaaggcgcg ccatggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     8941 cgcagataaa atgaacgctg cgtttcctga actcaactgg cagatcccgc aatccactta
     9001 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     9061 tatcgagcaa gaaaagatcg cgatcatgcc gggatatacc tacggtgaag aaggtcgtgg
     9121 ttttatccga ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     9181 aattaacgcc atccgcgctg ttggttaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     9241 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     9301 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     9361 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     9421 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     9481 tgagctttct gactaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     9541 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     9601 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     9661 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     9721 tcggtattat gagccggacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     9781 tagcccaccg tgaccagatt accgcacttg atttagccgg tgatgaactt ggtttcccgg
     9841 gaagtctgtt cctttctcat ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     9901 atgcaggcga agctgccgga ccggaaagca tctggcaggc gattcgtgaa ctgggggcgg
     9961 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
    10021 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtggcgg
    10081 atctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
    10141 ctgacgatcc aggcgtgcag ggagtggata tcattcacga atataccgtt gccgcgccag
    10201 ctgctgggtt atcccgcgag caaatccgcc aggcacagat taatggtctg gaaatggctt
    10261 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
    10321 ggatggtgct aattgcacca tccccttttc acgcaaggca caaagtcgcg cgatgtttgg
    10381 cggattcgat gcccagctca atcaactcca ttacctggat tgcctgactt gccggaaccg
    10441 gattttcacc atcgccattt aacgcatcac gaatagccgc atagtaagcc ggataattcc
    10501 ccggcactgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
    10561 catcgcgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
    10621 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
    10681 gtgctgactc ggctgctgcc agcatggtgc cgtgtaaaat cactcgccgc tgcggatagg
    10741 acaagatagc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
    10801 ccgtcatgct aaccggtaaa ccaaataacg taatggcctg atccagaagg tgcggtgcta
    10861 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
    10921 ggcggaagcg gtcaaaatgg gactcaaagt aagcaatttc acccagcacg ccttccgcga
    10981 gcaaaccttt tagcgtcagg aaatcgctat cccagcgacg gttatggaat acagacagca
    11041 ctcgccccag gctttttgcc agcgcttcca gctctcgtgc ttgtgacagt gtcacggtaa
    11101 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacggaaaat
    11161 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
    11221 tcggctcaga gacaaccgta accgttggcc agtcagcttt tacttttgtt tcatcgctgc
    11281 tggagattac cgccagctcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
    11341 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
    11401 tcattgaagt atgatggcta tttgacacta tcctttaccc tcgctcaaca gtttaataac
    11461 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aatagcgatt tttccaacga
    11521 caaaagctaa atattgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt
    11581 aacatttacc ccctgaagtt aatggatcaa tcaacagaga tgtgggctgt aatgaatcgt
    11641 cttattgaat taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg
    11701 agtcacattg acaactatca gccacctgaa cagagtacta cagtacaaca caagtaagct
    11761 ctgcacttgt tgagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta
    11821 aaccaggaat ttattatctt gttcgatgtt gttgatgatt gtcagggata gtaaagcgcg
    11881 ttactctttc cagagcagtt tttcatccct gaatatttgc catcagcaca ttcttattat
    11941 tgtaataaca caagcgttca ct
//
LOCUS       DAZFRG010000146        11949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFRG010000146 DAZFRG010000000
VERSION     DAZFRG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.146"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..484)
                     /locus_tag="ACHKUC_003230"
     CDS             complement(176..484)
                     /locus_tag="ACHKUC_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619439.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFY
                     LCPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLYLSSDKFIPVKRRK
                     "
     gene            complement(511..1503)
                     /gene="traU"
                     /locus_tag="ACHKUC_003231"
     CDS             complement(511..1503)
                     /gene="traU"
                     /locus_tag="ACHKUC_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO6619440.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(1500..2132)
                     /gene="traW"
                     /locus_tag="ACHKUC_003232"
     CDS             complement(1500..2132)
                     /gene="traW"
                     /locus_tag="ACHKUC_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO6619441.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(2129..2515)
                     /gene="trbI"
                     /locus_tag="ACHKUC_003233"
     CDS             complement(2129..2515)
                     /gene="trbI"
                     /locus_tag="ACHKUC_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO6619442.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(2512..5142)
                     /gene="traC"
                     /locus_tag="ACHKUC_003234"
     CDS             complement(2512..5142)
                     /gene="traC"
                     /locus_tag="ACHKUC_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO6619443.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGMSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(5268..5615)
                     /locus_tag="ACHKUC_003235"
     CDS             complement(5268..5615)
                     /locus_tag="ACHKUC_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619444.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(5643..5861)
                     /locus_tag="ACHKUC_003236"
     CDS             complement(5643..5861)
                     /locus_tag="ACHKUC_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619445.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIESHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            complement(5941..6414)
                     /locus_tag="ACHKUC_003237"
     CDS             complement(5941..6414)
                     /locus_tag="ACHKUC_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619446.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHKRIDNILAFCACIAWVPCIIA
                     TFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEESKSE"
     gene            complement(6407..6628)
                     /gene="traR"
                     /locus_tag="ACHKUC_003238"
     CDS             complement(6407..6628)
                     /gene="traR"
                     /locus_tag="ACHKUC_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO6619447.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(6763..7278)
                     /gene="traV"
                     /locus_tag="ACHKUC_003239"
     CDS             complement(6763..7278)
                     /gene="traV"
                     /locus_tag="ACHKUC_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO6619448.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTITATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(7275..7595)
                     /locus_tag="ACHKUC_003240"
     CDS             complement(7275..7595)
                     /locus_tag="ACHKUC_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO6619449.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(7582..8172)
                     /gene="traP"
                     /locus_tag="ACHKUC_003241"
     CDS             complement(7582..8172)
                     /gene="traP"
                     /locus_tag="ACHKUC_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO6619450.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCPVYFPDDRTNRSNGDKNEHA"
     gene            complement(8162..9589)
                     /gene="traB"
                     /locus_tag="ACHKUC_003242"
     CDS             complement(8162..9589)
                     /gene="traB"
                     /locus_tag="ACHKUC_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO6619451.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(9589..10317)
                     /gene="traK"
                     /locus_tag="ACHKUC_003243"
     CDS             complement(9589..10317)
                     /gene="traK"
                     /locus_tag="ACHKUC_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO6619452.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(10304..10870)
                     /gene="traE"
                     /locus_tag="ACHKUC_003244"
     CDS             complement(10304..10870)
                     /gene="traE"
                     /locus_tag="ACHKUC_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO6619453.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(10892..11203)
                     /gene="traL"
                     /locus_tag="ACHKUC_003245"
     CDS             complement(10892..11203)
                     /gene="traL"
                     /locus_tag="ACHKUC_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO6619454.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIIT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(11218..11580)
                     /gene="traA"
                     /locus_tag="ACHKUC_003246"
     CDS             complement(11218..11580)
                     /gene="traA"
                     /locus_tag="ACHKUC_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO6619455.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLV
                     GFAILSVFIAVGMSVAGF"
     gene            complement(11613..11840)
                     /gene="traY"
                     /locus_tag="ACHKUC_003247"
     CDS             complement(11613..11840)
                     /gene="traY"
                     /locus_tag="ACHKUC_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO6619456.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
BASE COUNT         2888 a         3269 c         2800 g         2992 t
ORIGIN      
        1 ggccattaca tcaaaagcac tcaccctttc cagcagggag aaaaaacgct cctcactgat
       61 aaaaatttct ttttctttat tacttatctt tcgtaaaaat gataaaaact cataacacac
      121 ctttatttat attatacata tacacacacg gttatattta tgagaaccga cagactcatt
      181 tcctgcgttt aacaggtata aacttatctg acgaaagata caaaacaaca acgcttacca
      241 ctgtcagaat aagaaccgtc ttcagataca gtttgacaag cagtattttt tccagtgtat
      301 ctgcatcctg ccagtcgtta acaatgtctg agataatatg ttcagggcaa agataaaacc
      361 acaccacagg gcatatcacc atagcaaaag gaaacagttt taatacattg cctgtcaaac
      421 gaatgaacac tcgggatata ctcatataca tatgaatgta tttactgctt aatttctttt
      481 ccatttaaaa tattcctctt tttgtttaag ttacaggaat acacagttac gtttacgcca
      541 catcaggtag ccgaagtttt tctttgtgtt gggcggattt tttcccgtct cccagcgcat
      601 cacactgcgc ccgaacggat ggcactgccc gctgtccgga tacatattca ccatctgata
      661 acgccagcgt tctttgggca ggattgggga cggatattca ttacagacgg cgttattttt
      721 cccgatggtt tccataatca tgccctgacg gtgtagtttg aacgccatgc gttcactgac
      781 cagcagggag gactgcaacg gactggactc attactcacc cagccattga acgggtacat
      841 acttccctgc gaaccggcac accagaacag aacatcgaga ggcatattaa aggcgctggc
      901 aatggcatct gctgcacagg ccccctgtgc tatcggattg gcaaagatga cagcttccgg
      961 attgagaatg gtggtcaggc tgctgtccgt ccaggtgggg tcaatttcag aaagataagc
     1021 gatatccagg tcaccacctt ccagacagcc cagcgatgtg atgatgttca gccagtacgt
     1081 cagcgggtat ttgtaccagt gaacgtgata gaacgccccg ttcacctgct tctcatcctt
     1141 tttcgccgtt ccctgtgccg ttttaccaaa agccggcagg ctgaagccca ggttaaccat
     1201 gcatcccggt gaccgggtga cgtccgtcag cgccatcggc tcccagtaac caatggccag
     1261 cccgatacgc ctgaacagcg gcggcggtgc cggacaaatc tgaatgggca tcgacgggtt
     1321 cgccgtgtcg gggaccttac cctgactgac tttgatactg cccagcgaga gcgggaaaat
     1381 acagctccag cagatatccg tgatcgggtt tacaaaacgc ccctcacagg cagaatccgc
     1441 agacgctgca gggacatgac cggcgagaag gaataacatc agcagccaca ggcttcgctt
     1501 cattttctgc cctcctctgc cggaataaat tccaccttca ggaagcgatc gccgggaacg
     1561 gcgctcaccc gtgccgggac ctgatcaatg ccgaggcgct gacagagcac accgttctgg
     1621 tcaaagtaaa cacggctgtc cagagacttc tgcatctccg ggatactgcc ctgcacgagg
     1681 ataattttgc tctccagtgt gggcggcgtc tggcgtttca tccaggccac ctgcgccgga
     1741 tcatcgccat tgatgaaata cagcgtctgg ttaaaaggaa catactgcag cggattcatc
     1801 acttcaccct gacgggcaaa cacccgccct tcgttatccc ggatatccgc agccagtctg
     1861 acggacgggt caaacaaccg gctgctgtat ttctccgtgc gtccgatacc gggcacggca
     1921 gggggccgca gactgttgcg gatcacccgc tccttaaagg catccatttt ccggcccatc
     1981 tcaccggact gctccagtgc cgtcaggcgc tgcatgatca ccgtcagcat gtccggctcc
     2041 tttaccggcc acagatcgcc ccaggtgcca agatcggcgg cggccacact ctgcccccat
     2101 atcagcaggg caatcagccc ccggcatctc atggttccgc cctcattcgc cgggcaatat
     2161 cctgctggat ttcccgggtg atatccggag ccccctgtac cacggcaggc gacaccagaa
     2221 tgaccgcgtg gtgtttctgc tgccatgcct gcaggctggc ttccagtgcc gtgttaaaac
     2281 gcgctgaaag ggcttttgac tgtgcttctg acagttgttt ctgactggcg ctgtcaaaaa
     2341 acgcatccac ggtctgtttc atgttgaagg cggcagtcac cggtgcgttc agtctgacaa
     2401 tgccataact gatggctgcg ttcagtaaca ccattgccag gcatcccggc acggtccacc
     2461 accagtggct gcgtcggggg gctgtaacgt cagtcgtttt ttgcgttgtg gtcatgcaac
     2521 actcctgtat ttctcatgtt cttccagcca ggcctccagc gaagccatct ccggccccga
     2581 tttcttccac gccagctgcc acaccgcctc atgaatactc ataccctctc tgcgtttctg
     2641 ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg gctcggctta acgggtccac
     2701 aaacagacgg tgccaggagg aatggttttc cacctgcagc aggaaggaac tgaaccactg
     2761 gtctttggcc gcaccaaact taccaatcat gtcgcgctgc agtggctgga actgatccgg
     2821 gaataactgg ttatatttcg cgaactcctt cgcactctgt ttgaggataa ttttgtagga
     2881 ggagttaccc catgccgcac gggctgcgct ggatgccttg tcagagtcaa agtcgacgat
     2941 gttctgtgtg atggtgatat aggcaccggt atgacggcga caggtgcggt aacctttttg
     3001 aataaactca ccaactttac gatttttgaa gtccagcaga cgccagcctt catcaataac
     3061 gttcagtttc ttgaggttac gcggcgtgcg gtacatcctg ttctcgatgt agataatcag
     3121 ggagaacatc accgcaacca gcagtgacgg acggtcttcc agtccgccca gctccagcac
     3181 caccattttc gcatcatccc gcagggacgg ctcatcggag ttaaaatact ggccgtaagt
     3241 gccgttggca gtgtactggt caagcagcac aatcatttcg tccagacggc tgcggatggt
     3301 cggtgacccg gcatactgct cgctgtcact ggcgtttttc aggaaatcca ccacgtcatc
     3361 aatacgtgcc ctgttctctt tggccagcca ggaggccctg accgcctgca gcagcagacc
     3421 ttcatgcact tcatccaggt taccgttggg gctggccatc actgacaact ggtcacggac
     3481 acgctccgct gactggtcaa tatcggtgat gttcgcaaac gggttaaaac gcagggtttc
     3541 accgtccaga tatacgccac ccatgttctc acacagggac ttgtagccat cccccatgtc
     3601 gaacaccacg gcaaagcccc cggagtccag cacgctgcgg ataagtggct gtatcagccc
     3661 ggttttaccg gccccggagg tgccacagac cgccatgttg tagttggtgt tattcattcc
     3721 ccggaagaaa atatcgataa acgccagctg gttacggtag gtgggtgcca gcagacctgc
     3781 cggtgtcagg gggttatccg ccactaacgg catcaggttg gcaacattaa agctctctgc
     3841 gcgctgaacc actccggcct ctttcagctg tttaaacaac cctttcccgg ccataaaggg
     3901 cagacaggtc aggaaattgc gcatgtggtt aaagcgcggt gaaatcagct caaaaccgtt
     3961 tttacggaag ctgttcagga tgtcctgctc cacttccagc gccgtttcat tgttgtcctt
     4021 gcagaatgct gtgatgttga ggaagtagga cacgacagaa gactgaccgg agcccagacg
     4081 ctggcgcagt tccccccact ccttcgcctc tttctccacg gacggaaacc atttggcata
     4141 ggaggtcttc gactttttct ccaggtccat gtacttcagg ttggcttcgc tgtgggtttt
     4201 cacctggtct tccaccacca gagtcagcgt caggatgaac ggacaggaga tggacagttc
     4261 cgggttcagc aggttgctgt agttgtcggc catattccac aggaaggcga tttccgggtt
     4321 acgggccaga tggaaattca ggatacgggc cgtgctgttc ctgccgttct cacgcaggcc
     4381 cagcgtcagg taatcagccc ggacattcag gtcaaaactg tcctccacac actgataatt
     4441 cagatcagaa tacgggtcca gctgacgtct tttcgggtac agggagtccg ggttatgatt
     4501 aatcatctcc ccgacaatat cgataaaggc ctgtgcatcc accgtctgtg tggcgatact
     4561 ggccccctgt aacgacgccc ggatgatttt caccaggttt tccatttcca gaatgtcagc
     4621 ccggcttttt ttcttcgagg gagaacagta cgagataaat acccggtaat ggcgcagggt
     4681 caggggcaga ttcatcccct ccggcagcgg aaactgtgtc gctgccgctt tcatataata
     4741 ggcacgggta atggcgttaa accgttcagc ctgttcacct gaccaggaga actcacgcag
     4801 cccgtattca atcctgtcac caaccagctg actggacatc agatggatac acaacggaat
     4861 accgcgcggc agtttggtgc gcagcatgtg atcgagtgcc tccacaatgg acttattcgc
     4921 cccattgatg ggaatggctt ccagcataaa gcccatcgtg gtgtcattca tgaacagacc
     4981 ggattcctgg ttgtaatcac ggtacggcag cagacggctg aactgcggga agctcatttc
     5041 gcccagaact tcattggcct ttgcggattc gtcaggcagt ttcagtgctg tgacgaggga
     5101 gttaaccgcc tgagtgacgg cctcaagtgg gttattactc atatgagtat tccttagtgc
     5161 gtttaaaaaa ggaaaatatc agtctgtggc cgtggatata tcacgcagtg ccattcctga
     5221 aaccacagca caaataagag atggaggctt ttaacaggaa accaggatta acgttccttt
     5281 actgaatgag catcatttaa tgcctgaatt tgtttttcgc gaatcttatt gcgctcattt
     5341 tctcttaccc gtttcacctc tgccatttcg tttctttttc tgcattctga tgcgatttta
     5401 tttgccttac caagtgttaa aggttcagac tgataattaa gtgtctcctg aaatgcttct
     5461 gctgcacagg gagtttcctg taaaatctct gtcagttgtt ctggtgttgc aggttgttgc
     5521 gcaaggtaaa cgatgtaaaa aggaatacat acaatcatag ccataacgcc aacaaatata
     5581 aaagccagaa cagattttaa atatatcgac ttcatttcat tttccttcga agcagtaagc
     5641 actcattgcc caccaccatt tagtgtttcc atcacgctgt tcaaaggttc atttgtttta
     5701 acttcaatac actcccctgt atttccaaat cccctgcctc cgtgtgactc gattgtccga
     5761 agaacctgca cgcatacgtc cgtatttggc atgggtgtaa tattccagct tccgccaccg
     5821 ttaatatttt ttatcagcac aagaaaaata aaggtatgca ttctttatta tctccgcttg
     5881 tatattaatc actgaatatc ccttcagtta acttatgagg tcataaaaca ctgcagattt
     5941 tcattcagat ttactctctt cagtaaactt ccggatggta tcctgttcac gctgacgggt
     6001 ggcagactcc tgcattgcat ccactttctc ctgccagaga cgccggatat ccttttcatc
     6061 ccgaccagtc agttttttac cggacagcag acggttaagc agttcctgtc tcgcatccgg
     6121 tacgtcttca gtcagacgca taagctgaag caggctctga cggctgagaa ccatgagtga
     6181 ttccggcagg agggtgaaga agaaaataaa aaagcatacc gcaccacagc aggcaaggac
     6241 cgcaaacagg attttcagat atataccatc aacgccgaac gtagcaataa tacagggaac
     6301 ccatgcaata cacgcacaaa acgccaggat gttatctatt cgtttatgtc tgcgatatat
     6361 ctgttgatgt tcatccagaa tagaatctat tttttcagct tctgatttat gcatagtgtt
     6421 tcctctgcct ttcctgatac gcctgacatt caacgcacaa cgtcacaccg ggaaatattt
     6481 tccgccgggc ttccggaata ggatttccgc atgcttcaca gagataaaca ggaataccat
     6541 gaacatttat tttctggcgt atccggttta ttcctgtcat ggtcagttgt tctgtcactg
     6601 aatatgcttc atcggcttca tcactcacgg aatattcctc ccgacatgtt ctgacacgct
     6661 accgcccatc ccttttcacg cacagggcac tcgttttaca ggcagatttt gaatacagaa
     6721 agcccgccga cattgccggc tgatatacaa cttcagggcc agttaattaa tacgtggttt
     6781 tccccacgca gacggtttaa taacaaaaaa cacgctggat ggctgatgat aaacatcctg
     6841 gttatcaata taaggcgcta tccataatgc tgccgtctgc tcacccgttc ttagcggacg
     6901 aggaggtgtt accggcgtaa ctgaactgac cggaacaacc gttttcactt cccgggcagc
     6961 ggtaaacagc gggcgtggtg ccagcagttt ctgctccgga tgtgctgtca ccgcaggtct
     7021 gctgccggac ggagtggtcg cggtgatggt ctgcaccggc attgtccgga agttcccttc
     7081 agccaggcgc ggcagtgatg ccgcaaccgg ctttgcttct gaggactgct ccagtttttt
     7141 ggccttctca ttggcctgct ccatcgtcat acaggtatcg gatgtggtgg cgttacactc
     7201 aaattccgta tttgttcctg cgcagccgga aagtaataag gttcccagca gaggaataaa
     7261 taaagaaatc tgtttcattg cccttctcct tttttattac tccccacaac agcagaaatt
     7321 aactctgctg tcagatttgt tttccctgca cagggatgtc cggagaccac tgtatttccc
     7381 ggagaagaaa ataagctcat cgacattccc ttcttcatca caccgacagc agccatatta
     7441 aatttgtgcg gcaatgattc agaactgaac accttcggag caggaagaac tatttttgtg
     7501 gtgcagttac tgagaacagc atgcataaaa tcatctgata cgtttttatc cggcacaacg
     7561 cgggctgtaa taacattaat attacgcatg ttcattttta tctccgttgc ttctgtttgt
     7621 tcggtcatca gggaaataaa cagggcattt cctgattttt cccggcaggg aagcgtcttc
     7681 cgtataaacg tccttcagat attcatctgc ggttaccacc cggatacatt tcatttttcc
     7741 gggcagcacc ggatatttcc cggtgatcgc tgccagcgag acatacatca gtgccagtgt
     7801 tgcccataat gcggtgacca gttgcatcag tgaccggtca gtctccctga cataatctgc
     7861 agcatcagcc gcgcggtaat ggcagatatc ctgtatttct gatctgcctg aagaatcaga
     7921 ggaagaaaac cagcattcac tgaccgggaa ctgtccggca ggtgctgttt gtcttacgaa
     7981 gttaatctcc ttaaccagta atttccccgg cgtggtgttg tccttccata aaacaaacag
     8041 agccatcagg gccattgtgg ccagtggcag aatgacggtg tacttcagac accagaacag
     8101 tcctcgcaga acccgggcga caacataccg ggcggcatgg cacgcctgac gggacgacat
     8161 attatttgcc atcgttgcct cctccctggc tgccggacat aaactgctgc ggattcaggt
     8221 tgcccagctg cttcagcatc tggtcagtat taaagccgtt aagatgactt tcagctgatg
     8281 gcggaacagg aaccggactt tccggattat cttcttccgc tctgttctgc gtgcgctcca
     8341 gcgccatctc ttccacggtt ttcagctgga agccgtcctg gaacaccacg gtcacttcgt
     8401 tgcccgcacc aatcggtatc accgggtgat actgttcggc acgtttgatg tagtagtcac
     8461 tgagcgtctg tgcggctttc gatgcgccgc caccgatacc cattttcagg acatcaccag
     8521 ccccgtaagc ggctgtggca cccagcccga cagccggctg ggaggcgcgc tccattccct
     8581 gaccgatacc gtcaacaaat cccgcgcccc atgcccagcc gaggattttg ccgttacgca
     8641 tcaccacttc gcccttgata ccgtttttac cccggaagct gacatgcccc ttaatcggca
     8701 tatcaatagt tttgccgtcc ttcaggcaac tgatattgcg ggtgcgtaca atggcacgtt
     8761 cactggacac atccccccag gcttccagcc ccacaaaaca tcccgttgcg tcatacgtct
     8821 tgctgttcgg catttccacc aggccggtga tacgcaactg catcggcacc gtggattcat
     8881 taccggtgac tgaggcattg gcatccgccc cttcaatcag catggctttc gcaaaacttc
     8941 ctgacggaat gtacggcagc gatggtccct gttttccctc attacgggta aacaccttac
     9001 gctgtatccg gttaggcacc ggcacagact ggtacgtcac ctgtggtggt ggcgtgacac
     9061 cattcccggg ataaaacgcc gtcggcggtg gaacagcaac gctgccctgc ggcgggaagg
     9121 atacgggggt gtttcctggc tgtggttcgc cttccggccc gggcggtgag gcaggcatct
     9181 gaggtacagg ctcaccggtc gccgtgacgg gattagcacc cagggctttt acctgctctg
     9241 ccagggcggc attgtcctgt cccagctttt caatacgacg ctggtcatca ccgcgctgtt
     9301 tgttcaggac atccagttca cgacggattt cctcatactg cttctgcatc tgcgctgccg
     9361 tcacctgcat ctctgtggtg gcatgctgac gcaccttgtc atcaaaggtc gtatccacca
     9421 cacctgtcat atccggcaca ggctcctgtt cagccaccgc ctcaccgtta ccggacatgt
     9481 ccacatcaga cagatacagt accccaccaa tcgcggaggc tgcaccgaca accacaatcc
     9541 ccagccacag gtactgcttg cgtttcacaa tggtattgat actggccatc agttgccctc
     9601 cccgtcacgg atgacataca catccatgcg cgcacctgcc agtaactggc tggcaggctg
     9661 gctgaacatc acggcacggg ttcccggctg ccagaaatca ctttcccgga tattcagggc
     9721 agacagcgtt ttattttcca cggcaaagcg gaccatcttc aggtgattcc ccgtccatac
     9781 ggaatctgcc actgaagaca gccccgccgg ggcctgcagg gtttcctttg tcacaggaac
     9841 ctgataccag cctgcaggta attttccgcc acggacagcc tggctgatgg tcacaagcag
     9901 ggattcgtaa ggcgtggacg tttcccacgc tccggcttct tctccggttc cgcgcaggtc
     9961 actgaccagc tgaatggtac gccccgcgcc ttcacgggga acagcctgaa tggaaaggtt
    10021 cagaccacgc tctgtttcca gaatgaacgt aaagggcttt ttgttgacgg tggccaccac
    10081 cacaccgccg gaggcggtct gctcattatt ggtcagggca ccatccagac tgttcacggc
    10141 gataatccgg tcaccgggaa cggtaaacag attcgggctg gtattgctga tggcaatact
    10201 ggcctgacca ccgttcacca ttggcaccac agtgggggcc agcgtaccgt ttgcggccat
    10261 cacgctgcag gaaaaaaaca ggccgccgaa tattattgcc gtattatttt ttctcatcgt
    10321 ctgtttcccc gaaattatcc agccaggtca ccccgttttc ccgcttcaga ataaggatgt
    10381 aatgtttgat atcagtaaaa ggttcggagt taccaatcca tgttttccga attccacgga
    10441 tttccacacg accatactga ggccagacac ggacactggt ctggaaaaaa gcagaattca
    10501 cgttatcttt tttaatgagt ttcgcttctt cagccagaat aactttcatc tggttctgtg
    10561 cacccgggcg gatatattgc agaagcgcct gatgtgaagc atcgacgatt tccggtgaga
    10621 cattcagacg gagggcaata aatgacagcg ccatctgctg caaataagag gcatcggcac
    10681 tgttctgtga cacggcaaag ggcacattaa atcccattgg ggtcacggca gtgcgttgct
    10741 cattctgaag acggtagtta ttcaccccct gaatgaggtt aacagagagg ctgagaacaa
    10801 taagcactga cataaatata aaggcgatgg ccattacacg actggtactt aaacgggcac
    10861 cgtgttccat ataacaatcc tggtatcagt tctatttaat ccactgccgg aaacacgaat
    10921 cgggaacatt atgaaaaata ccgcgcagca gggctgttgg catataccag taaatcaggt
    10981 cacgtaacca ggaactgccc cgcccttttt tcagtttttt aatcccgaaa taaaccagaa
    11041 ccgctgcacc aataccgaac agatatttcg atgttatgat accccagcca atacagattg
    11101 ctgcggggat cagttcatcc agtggcaggc caaaccagcg gctctggttg gtcagtgttt
    11161 ccgggaaacg atatttctta agtttattct cgtctcccga catcgtttta tttcctgtca
    11221 gaatccggca acagacatac ctactgcaat aaatacagag agtatggcaa agccgaccag
    11281 gaacttgacg tttttggtca tcatgtacat gactgcaccg accagaactt cagccagaac
    11341 aacccattta acgatactgg agtccttacc gaaggtcgcc ttaaccgttt cctgaccttt
    11401 agccatcagg tccgtgcctt taccagcagc catagctaac tcagggaaca acatcatcag
    11461 aacagcaacc gggatcactg cacgaaccag gcgaagcata ttcagacgag tgaatttaga
    11521 aaaaaacgat ttcttttcaa cgggcgcaga agcaccctga acacttaaaa cagtattcat
    11581 aagttacctc tattgtggat agattaataa acctaaagat ctttaattat cgaatcagtt
    11641 tcctctgctc cctcctttat tgcatccaga ttataaaagt caggaaaacg gttcagatga
    11701 tcacgtaacc tgactagcac ctcattagtt tttgttctcc cactacgttc ccgggcacct
    11761 aagagcttgt gattagtagc atcgtccaat accagtaaaa ctttattgcc cggagcaggc
    11821 cttattatat ttctgctcaa gtctcctcct cagccactta gataattaaa tataattata
    11881 cgaatccata aaaatagaaa cgaaaataga gtaaattaca ttaacaaaac aaggtgacat
    11941 aatgtcacc
//
LOCUS       DAZFRG010000147        11864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFRG010000147 DAZFRG010000000
VERSION     DAZFRG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.147"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3867)
                     /gene="entF"
                     /locus_tag="ACHKUC_003248"
     CDS             complement(<1..3867)
                     /gene="entF"
                     /locus_tag="ACHKUC_003248"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO6619457.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHITAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDIPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAASQDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMTPEQAWAPWIAEL
                     DIYRQDCAHVDIISPVAFEKIGPIIRA"
     gene            complement(3864..4082)
                     /locus_tag="ACHKUC_003249"
     CDS             complement(3864..4082)
                     /locus_tag="ACHKUC_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO6619458.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQSQ
                     SQASCQQWLEAHWRTLTPANFIQLQEAQ"
     gene            complement(4085..5287)
                     /gene="fes"
                     /locus_tag="ACHKUC_003250"
     CDS             complement(4085..5287)
                     /gene="fes"
                     /locus_tag="ACHKUC_003250"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO6619459.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQASLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5530..7770
                     /gene="fepA"
                     /locus_tag="ACHKUC_003251"
     CDS             5530..7770
                     /gene="fepA"
                     /locus_tag="ACHKUC_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO6619460.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7945..8604
                     /gene="entD"
                     /locus_tag="ACHKUC_003252"
     CDS             7945..8604
                     /gene="entD"
                     /locus_tag="ACHKUC_003252"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO6619461.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVHALREYGLKGVPAIDHHRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTAGELTDNIITAAEHEQLAGCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLHYQIGNCQHQQMTISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(8606..9541)
                     /locus_tag="ACHKUC_003253"
     CDS             complement(8606..9541)
                     /locus_tag="ACHKUC_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6619462.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGAAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGIKLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(9534..10364)
                     /locus_tag="ACHKUC_003254"
     CDS             complement(9534..10364)
                     /locus_tag="ACHKUC_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6619463.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEGLKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALKELKDA
                     "
     gene            10727..11677
                     /locus_tag="ACHKUC_003255"
     CDS             10727..11677
                     /locus_tag="ACHKUC_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6619464.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDATDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
BASE COUNT         2864 a         3239 c         3062 g         2699 t
ORIGIN      
        1 cgcacgaata atcggcccaa ttttttcaaa cgccaccgga gagataatat ccacatgcgc
       61 acaatcctga cgatagatat ccagctcagc aatccacggt gcccaggcct gttcaggagt
      121 cataccttcc tgaagcgtgc gttcggcaac aaatagcgtc gctttaccat caaacgctac
      181 gctatgagct gtcgtcagca ggcgcacagc atcagcgtag ttgccttcaa tggtggtaaa
      241 caactccgtt gaagtacttc cctgctgtgc cgccaggaag gcttcgcgct cgcggttaat
      301 ctccgccagc acttccgggt ccagaccatt ggcttctttt tcctgccagt tttgcgtttc
      361 tggcggccag gtatccagca agccaagaaa tgccacctgt tcgccacggg cacgcagtcg
      421 cgccgcaatg ccctgcgcca gcgtaccgcc aagtgaataa cccagcaggt aatatgggcc
      481 gtgcggttgt tgttcaagta aggttgccag atgcgcttcg cagacttcat cctggcttgc
      541 cgccgtctgc atggggccat gagggcgcgg cgactgaatg ccgataatcg accattgtgg
      601 atcgagataa cgcgagagca cgctgaactg ccaggcaaaa ccggatgccg gatggaaaca
      661 aaacagcgtc gggccattac cttcacgcaa cggcagaatg gtttcgaatc ccatgcgctg
      721 ggtgctgtct tcttcaccat caataatcgt tgccagtttg gcgacagttg acgcgaccat
      781 cacctgaccc ggcgtcacct gacgggcaaa ctgccgactt aactgcgctg ccagtttcat
      841 tgccagtagc gaatgaccgc caagcgcaaa gaaatcagca tcggcatcct gcacgtcaca
      901 acccagtaac gacgagaatg ccgcggcgat aatcgtttca ctgcccgctt tcggcgcacg
      961 ccccggcgct tgtgccttca gttcaggcaa cggtaaggct ttgcgatcca gcttgccgtt
     1021 ggcgctaagt ggtaactgtg gaagttgcag cagaaccacc ggtaccatat gtggtggtaa
     1081 tgtttcacga agctgcgcct gtaatgcgct ggtatccaac ggcagacctg attgcgacac
     1141 cagatagccc accaactgac gcgcatcacc accagtggct gccgcctggt taatcacaca
     1201 ggcgtgggta acggcctgtt cgacatccgg cagtgcctgc atcacgcgat cgatttcgcc
     1261 cagttcaata cgctgcccgc gaatttttag ctgatcgtca ctgcgcccga ggtactccac
     1321 cgcgccgtta tccagccagc gggcaacgtc tccggtacgg tacatccgtt cgccaggggc
     1381 aaaaggatcg gcaataaagc ggctggcggt cagatcgggt cgtccaagat acccctgcgc
     1441 cagttgaata ccggtgagat agagatctcc cgccacaccc ggcggcaccg gatgcatcat
     1501 cgcatcgaga atgcgcaggc ccgtattcca caccggataa ccaatcggca cactgctgcc
     1561 gcgcacctct gccagttcct cgccaaaagc cggataccaa ctgacatcta ccgccgcttc
     1621 cgtcgggcca tatagattat gcaacggcgc gccggttaac tgttgccatt cgcggcataa
     1681 atcagccggt aaggcttcac cactacagaa aacctgtttc aacgtcgcgc aactctggcg
     1741 agcggtttgc ggcgtcagcg atgcaacaaa tgccgccagc atcgacggca caaagtgcgt
     1801 ggtcgttacg ccatattcgg caaagaattg ctgcatagcg agcgggtcgc ggtgcgcttc
     1861 cggttcagcc atcaccagtt ttgccccggc aataaatggc cagaaaaact cccacaccga
     1921 gacatcaaaa ctgcacggcg ttttttgggc aacgacatct tcgcctgtaa gcgggtaatg
     1981 gttttgcatc caaagcaggc gattaacgat agccgtctgc ccgaccatta ccccttttgg
     2041 tctgccggtg gaaccggagg tgaagatgat ataagccgtg tggtgcggtt gtgaaagctg
     2101 cagcggcgca ctgccctgcg gtgtaagcgg ggcgttatag caaaggctgg ttaaattagg
     2161 gatatcgcta aagcgcggca gttgatcgtc agtggtgatt aacagcgacg gacgcgcatc
     2221 ttccagcatc attttcaggc gatcgtccgg atagccggta tccaacggta accaggccgc
     2281 accagcttca actatcgcat gtagcgccag ggtcaaaaag accgagcgcg gtagagccac
     2341 cgccacgctg tcgcccggtt taacgccgcg ctcacgcagc agattcgcca gcgccaccac
     2401 ctgctcgcgc atttcccgat agctgaactg gtaacgcgca tctgccagcg ccggagcatc
     2461 cggtgttttt gccgcttgtt ctgccaccag cgcgctcagc gtggtttctg gaatctcaat
     2521 ctgagtggcg ttgatctgcg ccagctgcgc atactcacct ggcagcataa tatcgacatc
     2581 gccgcacaac agcgccggat ccgcggcgaa ctgcgcaatc agcattttca ggcgttcagc
     2641 gtgttgaatt aacgttggct catcgtaacg ctgtttattg gcgaggattt caatactcaa
     2701 atcaccgtgt tcatccggga acagggccag ttcaaggtca ttaaccggac cggttgccag
     2761 ggtatgggtt tgcgcctgaa caccaggaat atccagttgg taatcaaata ccttgatatt
     2821 gagtaccgga ccgaacagcg gttcctctcc tgccgctcgc ccgctgtcac ggacaatttg
     2881 ttcggcatcg taacgttgat ggcgacgcat ttttttcagt tgagccgcca gtcgggttgc
     2941 cagttgcggc agcgtttctt gcgccgtaat gtgaataccc aacggcaaaa cgttgagcac
     3001 gggtccggta gccgtcagcg ccgccgagcc cagtcgacgc ataaagataa atccggcagc
     3061 gtagtccatg cggttgcaca atcgccccag ccacaaagct gccaacgcaa gggctaaatc
     3121 ggtacgctgc acacctgaaa gttgcgtagc cagctggcgg aattccccgt cggtaaattc
     3181 cagtttcagg cgcaaaatat ctgccgaagc gctgcgcccc ggtaaaggtg ccggagaaag
     3241 tgacgcgggc ggtggcagtt gacgacgctg ttccgcccag aatgccgcat cacgctgcca
     3301 ggcttcactt tcgcggtact gctgatactc ttccactaca tcagcgaaag gcgtaaacgg
     3361 cgaagcaggc gttggttcgc cacgcagcca tgcgcagtaa atattggcga tctggcgtgt
     3421 aatggccggg aaactgaagc catcgaccag caaatggtga taacgctgat accagtacca
     3481 gcggttatcc gccacctgta tcagctgatg aaagaccagt ggtttaccac tatcgacgcg
     3541 cagatcttgt tgcaaatccg cctgcattaa tgcccgcgca gtaccgtgcg gatcaatgtt
     3601 ggttcgcagg tcgataattt ctggcagttc gaacgtctgc gcatcatcga cccactgcca
     3661 gacttcgccg ttatcttccg taaaacgcat ccgcaacgta tctgcttgcg ctagtccggc
     3721 aaccaccgcg cgggccagta gtggcgcatc aacctctccg gttaactcaa cgtaatgcgc
     3781 cacgctccag gcggagggta aatccgacag tttttctgcc atccagatgc cgggctgtgc
     3841 ggcgaccaaa ggtaaatgct ggctcattgt gcctcctgca actggatgaa attcgccggt
     3901 gtcagagtac gccagtgggc ttccagccac tgctggcagg acgcctgtga ctgcgactga
     3961 cacacaacgt cccagcctgc cggtaaggcg cattgttgcg gccacagact gaattgcccc
     4021 tgcgcattgc gcaaaatgta aaacgctccc tgtggatcat cgaagggatt actgaatgcc
     4081 atattcaact cctgtcatgg aaaagtggtt gccagaggtc gattagcccc tgcatcaagc
     4141 caccgcgcca acaaagcgca tcatgtccgc cgtcaacctg acgccagaaa atggattctt
     4201 ttagggggtg taattgcgca tacagcgcct gattggcctg catgatcatc ggctcgcgaa
     4261 cacccgcttc cagcacaatg cgcagacctt cggcgctaac ttcaccagta tttagctgtt
     4321 caagtaacat gccctcttgg tgcccgcccc gatgcggcca ccagtacgaa cctgactggc
     4381 ttaatacaca gccaaagcgt tcaggccagt gcagtccggc atacagcgcg gaaagcccac
     4441 caaaactctg tccggcgacc acggtgcgat cggcgcgatc gctaaaaggg gcaatagctt
     4501 tcacctgggg taataactct tgctgtactg ccagccagaa atccgcatta cacggcagtt
     4561 cgtgggcgcg gtgcgtggtg tcgatagcgt cgatcaacac atacacggcg ggcggaagtt
     4621 gccgacgatg ggtcagcgaa gtcagcgcag gccagacggg catactctgc gcccaaaatt
     4681 cgccatcaag caaaactgcc agcgggcgtt cttctgctgt tgcatcgccg gtggtaaaaa
     4741 tccatacacg gcgtgaattt ttcaaccgtt cacttttcca gataatttct ttggcaggcg
     4801 tttctggcgc ttgcggacaa tcccatcccg gttgcagaga cgcttgcggc atttcgagtg
     4861 cagaaacagc gtgccctcgc ccgcctttcc agctttgtgg attcagcgga tcggctatcg
     4921 cctggggtaa tagttttcgc cagccttcgc gtaattcgag gcgatcgggg gatggtgcag
     4981 aaaaaatgtc atcgcgttca gtaggaataa agcagtagct gccacgccag ttggcattga
     5041 gttgtgtcgt ccactgccaa acatcggttc ctgcaattcg ctgcatcgac cggggctggc
     5101 tgttctgatg gtgatcggtc acgccagtga tatagaccca tacgcgcttt atcgtcgagt
     5161 attcttcaga accctgggga tcacgccacc agaaagtgac ctcaaacatt tcgtcattca
     5221 gacgttgcca ttccgggcca tgtttcgact gccaccagct ctcacttcct acttttaacg
     5281 ccgtcacacc ataaccccat gtttactgtg caatttttta ttgattgcag aaatatattg
     5341 ataatattat tgataactat ttgcatttgc aatagcgtaa tggcgcgccg tgggaagcgc
     5401 ggacattaat taatcagctg cactgcgtgt ctttcaggat caaaggtttt cgcggtagcg
     5461 ggatgcgtcg tgttgatgac gaccatgccc gacagttgca attcgtggca aaaatgcagg
     5521 aataaaacaa tgaacaagaa gattcattcc ctggccttgt tggtcaatct ggggatttat
     5581 ggggtagcac aggcacaaga gccgaccgat actcctgttt cacatgacga tactattgtt
     5641 gttaccgccg ccgagcagaa cttgcaggca cctggcgttt cgaccatcac cgcagatgaa
     5701 atccgcaaaa acccggttgc ccgcgatgta tcggagatca ttcgtaccat gcctggcgtt
     5761 aacctgaccg gtaactccac cagtggtcag cgtggtaata accgccagat tgatattcgc
     5821 ggcatgggtc cggaaaacac gctgattttg attgacggca agccggtaag cagccgtaac
     5881 tctgtgcgtc agggctggcg tggcgagcgc gatacccgtg gtgatacctc ctgggtgccg
     5941 cctgaaatga ttgaacgtat tgaagttcta cgtggtccgg cagctgcgcg ttatggcaac
     6001 ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag gcagtggtga gtggcacggc
     6061 tcctgggatg cttatttcaa tgcgccagaa cataaagagg aaggtgccac caaacgcacc
     6121 aacttcagcc tgaccggtcc gctgggcgac gaattcagct tccgcttgta tggcaacctc
     6181 gacaaaaccc aggctgacgc gtgggatatc aaccagggcc atcagtccgc gcgtgccgga
     6241 acgtatgcca cgacgttacc agccgggcgc gaaggggtga tcaacaaaga tattaatggc
     6301 gtggtgcgct gggacttcgc gcctctgcag tcactcgaac tggaagcggg ctacagccgc
     6361 cagggtaacc tgtatgcggg tgatacgcag aacaccaact ctgacgctta cactcgctcg
     6421 aaatatggcg atgaaaccaa ccgcctgtat cgccagaact actcgctgac ctggaacggt
     6481 ggctgggata acggcgtgac caccagcaac tgggtgcagt acgaacacac ccgtaactcg
     6541 cgtattccgg aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa agcggcacag
     6601 gattttgtag atatcgatct tgatgacgtg atgctgcaca gcgaagttaa cctgccgatt
     6661 gatttcctcg ttaaccaaac gctgacgctg ggtacagagt ggaatcagca acggatgaag
     6721 gacttgagtt ccaacaccca ggcactgacc ggaacgaata ccggcggtgc tattgatggc
     6781 gtgagtgcca ccgaccgtag cccatattca aaagcagaaa ttttctcgct gtttgccgaa
     6841 aacaatatgg agctgactga cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac
     6901 agtattgtcg gcaataactg gagcccggcg ctgaacatat cgcaaggttt aggcgatgac
     6961 ttcacgctca aaatgggcat cgcccgcgct tataaagcgc cgagtctgta ccagactaac
     7021 ccaaactaca ttctctacag taaaggtcag ggctgctatg ccagcgcggg cggctgctat
     7081 ctgcaaggta acgatgacct gaaagcggaa accagcatca acaaagagat tggtctggag
     7141 ttcaaacgcg acggttggct ggcgggcgtg acctggttcc gtaacgatta tcgcaataag
     7201 attgaagcgg gctatgtagc tgtagggcaa aacgcagtcg gcaccgatct ctatcagtgg
     7261 gataacgtgc cgaaagcggt ggttgaaggt ctggaaggat cgttaaacgt accggttagc
     7321 gaaacggtga tgtggaccaa taacatcact tatatgctga agagtgaaaa caaaaccacg
     7381 ggcgaccgtt tgtcgatcat cccggagtat acgttgaact caacgctgag ctggcaggca
     7441 cgggaagatt tgtcgatgca aacgaccttc acctggtacg gcaaacagca gccgaagaag
     7501 tacaactata aaggtcagcc agcggttgga ccggaaacca aagaaatcag tccgtacagc
     7561 attgttggcc tgagcgcgac ctgggatgtg accaagaatg tcagtctgac cggcggcgtg
     7621 gacaacctgt tcgacaaacg tttgtggcgt gcgggtaatg cccagaccac gggcgatctg
     7681 gcaggagcca actatatcgc aggtgccggg gcgtatacct ataacgagcc gggacgtacg
     7741 tggtatatga gcattaatac tcacttctga agctaacgtc agattgttga caaagtgcgc
     7801 gtagttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaatc ttgccaattc
     7861 aatatattgc aggaccacgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
     7921 tcatcattct ctaatatggt tgacatgaaa actacgcata cctccctccc ctttgccgga
     7981 catacgctgc attttgttga gtttgatccg gcgagttttc gcgagcagga tttactctgg
     8041 ctgccgcact acgcacaact tcaacacgct ggacgtaaac gtaaaacaga gcatttagcc
     8101 ggacggattg ccgccgttca tgctttacga gagtatggtt tgaaaggcgt acctgcaatt
     8161 gatcatcatc gccaacctgt ctggcctgcg ggggtatacg gcagcattag tcactgtggg
     8221 actacggcat tagccgtggt atctcgtcaa ccaattggca ttgatattga agaaattttt
     8281 tctgcacaaa ccgcagggga attgacagac aacattatta cggcagcaga acacgagcaa
     8341 ctcgcaggct gcggtttagc cttttctctg gcgctgacac tggcattttc cgccaaagaa
     8401 agcgcattta aggcaagtga gatccaaact gatgccggtt ttctgcacta ccaaattggc
     8461 aattgtcagc atcaacagat gacgataagc gggccgaatg gcgaccatat cgtgcagtgg
     8521 ttagtaaggg gtaataatgt tattaccttg tgcctggcag ataaacaccc gcacacagcc
     8581 gcatgcgggt tatctggcgg gtaattcaaa gcatttcccg tgcctgagct gcaattccgg
     8641 cagcatcaaa tccatgatgc tgacgcaacc acccacgatc cgccgctgcg gcatattctc
     8701 catccataat gcctgcgcgt tttaatttga tccccgttcc ggcctctgcc aacacttccg
     8761 cgaccaggct accaaggccg ccattgacgt tatgctcttc aaccgtcatc actgctttgc
     8821 atccttttaa cgctgccagc aatgctttgg tgtcgcatgg gcgaatcgac ggtacgctga
     8881 caactgtagc cgcgatacca gactgcgcta aaagttctgc ggcatcgacc acttcatgaa
     8941 cggtagagcc tgtggctacc agcgccagtt cgctgccctc gcgcaaagtc accacggcac
     9001 ctggtacaaa tcggtagtcc tcatcgtgga tttccggcag ggctttaccg tccatacgga
     9061 tataaacagg cccctgatag cgaagagcat aatcaatcac ctgacggcac tcccgaggtg
     9121 aagacggggc aaagatttga atattgccga aaccacgcat aaccgcaatg tcatcaatcg
     9181 catgatgcgt gctcgccagc ggtccataac tcgccccggc attcagacca aacaatttta
     9241 cgttggtaaa gttgtagcag acatcaacct taatttgctc gttagcgcga gcgataagaa
     9301 atggcgcggc attgcaggta acagccacct tgccgcccag cgccagcccg gcagcggcac
     9361 caaccatgct ctgttcggca atacccacat tcaccagtcg gtcaggaaat tgcttaataa
     9421 agggagcaat ttttgccgta gacgttgaat cagccactac aggcaccaaa tcgatcccct
     9481 catttacggc atcgataaat gcctgaacca tcacatttgc cagatgttca ctgttacgca
     9541 tctttgagtt ccttcagtgc ctgttcaatt tcatcgcctt tcggcacccg gtgatgccat
     9601 tccgcacgcc cttgtataaa ggaaatgcct gcccctttag tggtgtgggc aatcaccaca
     9661 ttcggtttgc cttcctgttt aagcccttca agcgtcgcga caaccgaagc catatcattg
     9721 ccctgacatt cggtaacctg cataccgaat gccagccatt tctctgccag aggatcggtg
     9781 ttcatgattt cgcgcgttgg cccggctaat tgcagattat ttttgtcatt aataatgacc
     9841 aggttatcaa gcccgtaatg cgccgccgcc agtgcagctt cccagttact gccctcagcc
     9901 agctcaccat cgccagtaat taagaagata cgacgacggc tttgactttt tttcgccgcc
     9961 agcgcaagtc ctacagccac agggaaacca tgtcccagtg ctccggtatt cagttcaata
    10021 cctggcgttt tttgccgaac cggatgaccg ggtaagtgtg aatttggctg ctgataagtg
    10081 gaaagccatt ccagtgggaa gaatcctgct tccgccagca cgcagtagta acatccaaca
    10141 gcatggcctt ttgactgaat gtatatatcc cggtcatcgt cgtgaaggcg atccggggcg
    10201 acattgagaa tacgaaaata gagtgcggtc agtaattcaa cctgagagag atcggcccca
    10261 gtgtgtccac ctgccgggct attggcattt aagcgcacga tatggcggcg taccgcccgc
    10321 gctttacgct caagggtctg gatatcatag ctatagggat tcatttaata cctctgaatt
    10381 tattttcaac atggaatatt tattccaccg gggactaaaa aaaccagaaa tcccagggcg
    10441 ttgcgcggca agacttacgc tgctgcatca tactcaggaa cattctgcct ccttacaccg
    10501 tcagttacat gtccgggtaa tcatcagttg acctgatatc attttgaata tatattcaat
    10561 aatgattctt tattcaatta tgccgcctgt tttttatttt tgtccatcgc tgattttaag
    10621 gattggttaa agttagaact ccatcacact taactgtctt cttttttcgc gtatgataaa
    10681 agcctctaag ataagggcgg tacgttaatg atttacgggg aaatatatgg ctaagcagga
    10741 tgaacagcgg ctactggtga agatcgccac tctttactat cttgaggggc gaaaacagtc
    10801 tgacatcgcc caacttctct cgctttctca gtcatttatc tccagagcgc taacccgttg
    10861 ccagaaagaa ggcgtggtta aaatcagcgt tgtccagccg tcaaatatct ttctcaatct
    10921 ggaaaaaggc attgaagagc gttacggcat taaacaagcg attgttgtcg ataccgaaga
    10981 tgatgccact gaccatacca ttaaacgtgc tatcggctct gccgccgcgc actatctgga
    11041 aacgcgttta agaccaaaag atttcattgg cgtctcttcc tggagttcta cgattcgcgc
    11101 gatggtcgat gaagttcacg cccaaaactt aaaagccagc ggtgttattc agctgctggg
    11161 cggcgtgggg ccaaacggca atgtgcaggc aactattttg acacagaccc tggcccagca
    11221 tctgaattgc gaagcctggt tgcttccttc tcaaagcatt gaaggttcgg tagaagagaa
    11281 aaaacgcctg gtggcaagcc aggatgtggc tgacgttatc gccagatttg acgacgtcga
    11341 tatcgccatt gtcggcatcg gtatccttga accctcgcag ttgctaaaaa cctcgggcaa
    11401 ctattatcac gaggatatgt tacaggtact ggcggatcgc ggtgctgtgg gcgatatctg
    11461 cctgcactac tacgataaac atggtcagcc cgtgttgcag gacgatgaag atccagtgat
    11521 tggtatggca ctggataaaa tcaaaaaatg cccgaatgtg gtggcactgg cgggcggcaa
    11581 agacaaagtt gccgccatca aaggcgccat gcagggtggt tatattgatg ttttgatcac
    11641 tgatttcccc accgccagaa tgctgattgc ggattaatcc tctcctccct ttcttctgcc
    11701 cgcctttgcg aaaaaatctt ctcgcaaagg cgcttgctta aaaagccttt aatatcaata
    11761 aactgaaagg attcaacaat cctgtttctc gaggctttct caccctgtca ccacgcttct
    11821 aagtgtgata gccagcacat ttgatgattt tttctctagc tcag
//
LOCUS       DAZFRG010000148        11823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFRG010000148 DAZFRG010000000
VERSION     DAZFRG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.148"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..2298
                     /gene="iroN"
                     /locus_tag="ACHKUC_003256"
     CDS             121..2298
                     /gene="iroN"
                     /locus_tag="ACHKUC_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HFO6619465.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDEDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKPPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGDALTTRLYGN
                     LNKTDADSWDINSPVGSKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDRDISGISGTAADRSSENHSQISALYIENNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTSNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2343..3299)
                     /gene="iroE"
                     /locus_tag="ACHKUC_003257"
     CDS             complement(2343..3299)
                     /gene="iroE"
                     /locus_tag="ACHKUC_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HFO6619466.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSATSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3384..4613)
                     /gene="iroD"
                     /locus_tag="ACHKUC_003258"
     CDS             complement(3384..4613)
                     /gene="iroD"
                     /locus_tag="ACHKUC_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HFO6619467.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAETLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKERVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNRRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSLPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4717..8376)
                     /gene="iroC"
                     /locus_tag="ACHKUC_003259"
     CDS             complement(4717..8376)
                     /gene="iroC"
                     /locus_tag="ACHKUC_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HFO6619468.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPVRLPWLACGLLLIAFFDFIGNYVRRGYAGILSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTHSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSDISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYAEEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSTIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFSHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLSAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            complement(8516..9631)
                     /gene="iroB"
                     /locus_tag="ACHKUC_003260"
     CDS             complement(8516..9631)
                     /gene="iroB"
                     /locus_tag="ACHKUC_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HFO6619469.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIRGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGLGADRPVNARVVAERGCGIIPGEVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            10241..10420
                     /locus_tag="ACHKUC_003261"
     CDS             10241..10420
                     /locus_tag="ACHKUC_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619470.1"
                     /translation="MAIFQEVGLPNNFLLIGFIAGDYYLLFVFPTKLLYNIEPSMNHC
                     WSHFTQTTAASSCDS"
     gene            complement(10456..11604)
                     /locus_tag="ACHKUC_003262"
                     /pseudo
     CDS             complement(10456..11604)
                     /locus_tag="ACHKUC_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612101.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2823 a         3151 c         3139 g         2710 t
ORIGIN      
        1 tgctaaatga tattaattat cattcttata taaaaacaat aatggcagta aaaaatatct
       61 ggcaaggatg tgagcttaac gatcaaacat tttaagttag gcatttatta gggaataggt
      121 atgagaatta acaaaatcct ctggtcgcta actgtgctcc tggttgggtt gaatagccag
      181 gtatcagtag ccaaatcctc cgacgaggat aatgacgaga ctctggtggt ggaagccacc
      241 gctgagcagg tattaaaaca gcagccgggc gtgtcggtta ttaccagcga ggatattaaa
      301 aagccccctc cggtaaacga cctttcagat attattcgta aaatgcctgg cgtcaatctt
      361 accggcaata gcgcctcggg cacacgcggt aataaccgcc agatcgatat tcgtggtatg
      421 gggccggaaa acaccttaat tttgattgat ggtgtaccgg tgacgtcacg taactccgtg
      481 cgttatagct ggcgtggaga gcgtgatacc cgcggtgaca ccaactgggt gccaccggaa
      541 caggttgagc gtattgaagt gatccgcggc cctgcggcgg cgcgctacgg ttcgggggca
      601 gccggggggg tggtgaacat cattaccaaa cgtcccacca acgactggca cggttcgctg
      661 tcgttataca ccaaccagcc ggaaagtagc gatgagggcg ctacgcgtcg cgccaatttc
      721 agccttagtg ggcctctggc tggtgatgct cttaccacgc gtttgtatgg taacctgaat
      781 aaaacggatg ctgacagttg ggatattaat tctccggtcg gttcgaaaaa cgcagccggg
      841 catgaagggg tacgtaacaa agatattaac ggcgttgtct cgtggaaatt aaatccgcag
      901 cagattctcg atttcgaagc cggatatagc cgccagggga atatctatgc gggcgatacg
      961 caaaacagtt cttccagtgc agttaccgaa agcctggcaa aatccggcaa agagacgaac
     1021 cgcctgtacc gacagaatta tggcattacg cataatggta tctgggactg gggacaaagt
     1081 cgctttggtg tttattacga gaaaaccaat aatacccgca tgaatgaagg attatccggc
     1141 ggtggtgaag ggcgtatttt agcgggtgaa aagtttacga ccaatcgcct tagttcctgg
     1201 cgaaccagcg gtgagcttaa tattcctttg aatgtgatgg ttgatcaaac gctgaccgtt
     1261 ggtgcagagt ggaaccgcga taagctcgat gatccttcct ctaccagcct gacggtgaat
     1321 gacagagata tcagcggtat ttctggcact gctgcggatc gcagcagtga aaatcattct
     1381 caaatcagtg cgctgtatat tgaaaataac attgagccgg ttcctggcac gaatatcatt
     1441 cccggcctgc gctttgatta tctcagcgac tccggcggga acttcagccc cagtctgaat
     1501 ctttcgcagg aattgggcga ttatttcaaa gtcaaagcag gggttgcccg aacctttaaa
     1561 gccccaaacc tgtatcaatc cagtgaaggc tatctgctct actcgaaagg caatggctgt
     1621 ccaaaagata ttacatcagg cgggtgctac ctgatcggta ataaagatct cgatccggaa
     1681 atcagcgtca ataaagaaat tgggctggag ttcacctggg aagattacca tgcaagtgtg
     1741 acctacttcc gcaatgatta ccagaataag atcgtggccg gggataacgt tatcgggcaa
     1801 accgcttcag gcgcatatat cctcaagtgg cagaatggcg ggaaagctct ggtggacggt
     1861 atcgaagcca gtatgtcttt cccactggtg aaagatcgtc tgaactggaa taccaatgcc
     1921 acatggatga tcacttcgga gcaaaaagac accagtaatc ctctgtcggt catcccgaaa
     1981 tatactatca ataactcgct taactggacc atcacccagg cgttttctgc cagcgtcaac
     2041 tggacgttat atggcagaca aaaaccgcgt actcacgcgg aaacccgcag tgaagatact
     2101 ggcggtctgt caggtaaaga gctgggcgct tattcactgg tggggacgaa cttcaattac
     2161 gatattaata aaaatctgcg tcttaatgtc ggcgtcagta atatcctcaa taaacagatc
     2221 ttccgatctt ctgaaggggc gaatacctat aacgagccag gccgggctta ttatgccgga
     2281 gttactgcat cattctgatc gtagtgtttc aggcgcactg cccgcgtaaa agcgggcagt
     2341 atttagtggc ttaactcatg acaacctgct gtgtaatttg cgttttcacc actgatatct
     2401 aacagtgcct ggcgaaagga tgcattgaac atcggcccgt gtcccaggtt ggggaaatcc
     2461 caaaatacgg cattgacgcc tttatctttc agtatagtga gggtggtatg aattttcgac
     2521 agcaccccga cagcatgcgt ttcccggtta tcaccctgtg tcgccgagcc ttccattatc
     2581 gccaggtgtt tggcgcagaa ttgcagaggc tcaaccgccg taacgcggct tagcaaagca
     2641 tcataacctc tgcccaacga cgggctggcg ctgtagtacg accggaagta agaggaagac
     2701 agccaggaat ccagcacgaa gaggccgccg taggagtgcc cccataagcc gcggcgttgc
     2761 cgatcgatat tcagtccctg ttccactttt ggggcaatac gcgtttccag taactggcgg
     2821 aagttgttgc tgccaccact cttacggcta aaacgccctg agtggagatc tgtttttctg
     2881 ctttctgctg ctggcgtata gtcgtaagcc ctgctgttga gatcgaaagg gaggttggtc
     2941 tgatacccga cagccacgat cactggcggt gttttttctg acaattgttt gagcagttcg
     3001 tcatccaggc ggtccataac tgcgttaccg tcaagcatat ataaaatcgg gtaacccgat
     3061 gccggtgcgg ttgtattcgg cacggccgtc catacccgat aatggcgtgt gccatcgaca
     3121 gagtcgaaag aggtggcgct gaaatggtaa aacacggagc ctttatcggc tatattcggc
     3181 cccagtggcc gcatatccgg cttcgcataa acttgcgcac tacagataag ggtaaggcaa
     3241 gaaagatgaa agaaaatcgc tttatgcggg cgtgttgagc gatactcgcg ggcatacatc
     3301 tgatgattat tccttttccg caggtgacaa tttgatttat tcaggaagtc tgtaccgttt
     3361 cctgaaagtg tttgtgggtc aactcaaccc tgtagtaaac caatcccgtc aatcagtgca
     3421 ccgcgccacc atgcgtaatc gtgaccaccg gtgtagattg cgcaatggct ttcgacgcca
     3481 gcggtaatca gccgctggtg aagttgctga acgtgaggca ctgtcgaacc ttccagcgat
     3541 cccacgcaca ggctgatacg cacgccctgc ggtggggcag aaagcagatg ctcactcacc
     3601 catgaggtat cggtttcgct gaacaagcct ggtagactgg ttctttctgg cgtccaccac
     3661 attgaaggag agtggctgag caccagaccg aacgtttccg gtgcgtaacg agcccccatt
     3721 agtgcactga tcccgccgag gctctgcccg gccagcactg tgcgcgaacg gtctgcccac
     3781 tgacgctgcg gttgttcagc gcggatcatc ggcagcagat gtcctgcgat atcctttatc
     3841 agcttgctgc gcccgccgag tatctcagtg cgttcatgtt cattaatgtt gtcaatgccc
     3901 agtacagcca cgggcacgat gcgcctgtta tttatggcgg cgtcaattgc cgcgcatacg
     3961 ccaaggtgat caaaccaggt ttcaccatcg ggcagcacga ccagcccgag gggctgagaa
     4021 atatcaacgt ccggaatata gagccgaacc tggcgagatt tcccggcgat aatgcgcatg
     4081 gaggtaagca gttgtccggt gtggaagccg ccgttccatt ccgacagggc gggagcttta
     4141 tcaagtgtca gcactgattc ctttgcgttt ccccgaacgt tgatctccgg cattttgttt
     4201 agcggatcgg cctttccggc gagggtggca aaacgacctc cggacagtgc aatcgtctca
     4261 gccgtagtgc cgggggggat ttccagcagc gaataggagc cgcagtaact tgcgggtaaa
     4321 cgcagtgtca gtgtccagat atccgtttcg ggaagggcgc tcatcattcc tttttctacg
     4381 cgctctttat ccgtcacccg gttcagacgc agataaacgc cctgcagagg atgtcgggcg
     4441 cgccacagaa aggtcacttc tcgttcatct tccgctcctt cgactggcgt cacaagagga
     4501 acattcagcg tctcagcttc gcgccagaag tcagtaaggt tagcaatgtg gcccgctgcc
     4561 agttggttgc gcaggctggc gatagcagag ggatgttgtt gcatgttcag cattcatact
     4621 ctttaaaaat taaggatgtc agtataaata attaaagcgg cttttcacgg taccgccgct
     4681 tctgacagtg tttttaatac attacgtgtc cagtaactat cccggggttt cctccttcat
     4741 gttgtgctgg cgatggagcg cagtactgct gacgctgtca tgccataagc gggtatagag
     4801 gccgcccgca gataacagct gttcgtgggt tccgtattcc gcaagtaatc ccttatcaat
     4861 aacggcaatc agatcgcagc gttgcgcggt ggtcagacga tgtgcaacaa tcagcgccga
     4921 gtgcttcccg gcatgcacga catctgttaa cgatgacatc agtcgttctt cggatgtgcg
     4981 atccagacat gacgtggctt cgtcgagcag caggatgtga gcattcgcca gttgcgcgcg
     5041 ggccagcgca atcaactggc gctgacctgc ggacagatcg gctccgccgt tattcaccgg
     5101 tgtccggaat ccctgcggca gattgcacac catctcatac agccctgccc gctgtgaggc
     5161 tatttcaacg tcttcattgg tggcggatgg ccgtgaataa cgaatgtttt ctgcaatatc
     5221 actactaaac agtgctacat cctgatcgac aagcccaatc tggcgacgat actcagtgag
     5281 cgaggcatca tcgagcattt gcacaccgat tctgatgttg ccgtgcgtgg ggaaatacaa
     5341 cccggcaatc agcttaatca gcgtcgactt acccgcaccg ctgcgaccga cgaccgcgac
     5401 aaccattccc tcagggatcg tcagggtaag tttgttcaga gctggctcgt gactgtcggg
     5461 ataactgaac gtgacctcat caagatgcag tgcaccggtg acagggagga ccgaagagga
     5521 cccgaggttc tcagtgcctt ctgtcgccag tagttcatca atatgtttgc cgctggctgt
     5581 cgcctgctgc caggcgtcga caatccctga taactgttgc actggcccat agaattgccc
     5641 cagcaacagg aagaaagccg ccagtacccc ggcagtcatt tctccagcgg cgacctgcga
     5701 agctcccact aacagaacgg cggcatagga ggcctcggtg cagaatgtca ggaacggaaa
     5761 atagactgca aggtattttt gtgctcgcac acgggttgcg cggaagcgct ccgataacgc
     5821 gcgcagccgg gcgccctcca gttcctgctg accatgcgat tggacgacac gcaggccgga
     5881 gactttttcc tgcagggtgc tgttgacttt gcctatttcc agccgtgcct gtgcataagc
     5941 aggggtactg aggcggcgat aaatcatggt tgccagtgcg gccactggca ctgcgcttaa
     6001 tgccgtcagc gccaggaacg ggtcgagcca gaacatggtg gcggcgattg cgaacatcgt
     6061 caccagactg gtggccgcac cggcaaggcc gttttgcaga aagcgggcga gagagtccac
     6121 atcaacggtc atgcgggtca gacgcgaatc ggcatgcttt tcatgccagg ggagtccaag
     6181 acgcagcata tggctgaagc tgcgcaagcg caccgaatgc tggactgatt ccgccgctct
     6241 ggcggcgaag accgtctgca gagaataact gcaccagccc acaatgacca ggcacagggc
     6301 gagcagggca cagaggccga tggtcgacat atcgcctgcg gcgacaccgg agtcaatgcc
     6361 atgctgcaac agtatcagca cccctacgcc tgccgcagaa tcgagtgcga tcagcagagc
     6421 ggcgatgacg aacatccacg ccacaggctt cagtagtgac gtgactttgc gacctttacc
     6481 ggccagcgcc tgctgtactg cattttccgg tacgcgggtc atacgggcga caaaaccttt
     6541 atcccccgta tccgttacat cgtcttgtgt cgctggttca gccggccaca attcgttgtg
     6601 gctattgtgg gacgtggcca gaaaatcgct gtcgccggtc atcagtgcgc ggaacgccgg
     6661 acagcgcgct tcaagttctg ccggggttcc ggtatccacc atacggccct tatccagcac
     6721 cacaacccgg ctggctagct gaagcgttga acgccgtcgg gcaatcacca gcagcatatg
     6781 cccttcttca gcatagcgac ccagcgcggt attaatctcc gcttccgtaa cagcatcaac
     6841 cgctgaggtg gtatcatcca ggattaacac gtccggtgca gtaatgagcg cccgcgccag
     6901 cgctattcgc tgcctctgcc cgccggaaag attcgtaccc cgttcggtta acaggctatc
     6961 gaagcctttc ggcagggcgt tgataaaatc gctggctcct gcagcagctg ccgcacggcg
     7021 aatgtcgtcc gccgttgcct gagggtgacc ataggcgata ttttccgcca ccgtaccggc
     7081 aaacagaaaa gcatcttcaa atacgatgcc tacccggcga cgaagcgcct ccagtctgat
     7141 atcgcgaaga ttttgtcggc cttcgctggt gttgagccat atctttccgg agcagggatc
     7201 ataaaaacgc gccagcagca tcaacagggt cgattttcct gaacccgatg cgcccaccac
     7261 cgccacggtt tcaccggcgc gcagggaaaa ggagatatcg ctgaggatat gtctgtcgcc
     7321 atggtggtag tcaaagctca tattctccag ttccagtccg acaacctgac tgttaatcga
     7381 ctccgtcccg tcctccatct gtgaatgggt gtcgatcagt gcaaacaccc gctccacgga
     7441 cgcctgagtg cgctgtgcga taaccagaaa cgatgccagt acgcgggttg gcccggtcag
     7501 catcgcgagg aagctggcga atgcaacgaa ggtaccgaga tcgatccgcc cgtgcatgac
     7561 cgaccacccg ccgtagcaca gcaggacgat ttgcccgagc accggtagcg ccagcatggt
     7621 ggccccaggc atcgcctgcg agataaccgc accgatgcgt acacgcacaa tctgccgcga
     7681 ctgcctatcg agccagtgcg tttcacgcat ctctgccaca caggatttta ccactgagat
     7741 ctgtgccagc acctcgcgta tatgttcggt gagattcgcc agttggtccg aggccagccc
     7801 ggtttgcgcg aagaccctac gacgcgcacg aagcgcggtg atcgccaggc ataccagtac
     7861 gcacacgacg ataagcgtca tggcagggga catccacagc atcacggcaa tgccggcaat
     7921 gtaataagtg aacactgcca gcggcaccgg gcacatctgc agcagggtat gcacctgctg
     7981 tagatcgctg ttggtccgtg aaatcacctg cccggtgcgc agcgcatcct ggcctgcgcc
     8041 gtcaagtttc tgaatactgt cgaatacccg tcctctgagg gtatgctgca cccagagtga
     8101 gagaatcccg gcataaccac ggcgcacata gttaccgatg aaatcaaaaa aagcgatcaa
     8161 cagtaacccg caggccagcc acggcaggcg gaccggattg cccgccagtg cgtcattcac
     8221 agcctgtctt gtcagcagtg gcgtcagcgc agccagcaaa atagtcgata ctgacgctac
     8281 caccacgatg acactaagtt tcttacgttc ccagcacacg cgtgccaggc gaactatcca
     8341 tgactgtgcc ggtgtgggag tgtgattcgc aggcattaag cctcctccgt ctgtaaaata
     8401 cgatccactg gccggatcgt tccgcaaaaa agccagcacg cttcggtccc gcattgttat
     8461 ggatgcatga ctcctggatg ggtgctggca tatgattatc atgaaggtgt agtggctacc
     8521 ctttctgtac cattgtgatc aggcttttcg ccacttcacc tgggcagggt tgcgccgcca
     8581 tttccgctgc tacctcttca gaggctttgc gaagtgagcg attgttgagg aaagcattga
     8641 tcatgttact cgacaggccg acctcgccgg ggataatccc gcagccgcgt tcggcaacaa
     8701 cgcgggcatt taccgggcga tcggctccca ggccgaagac gatttgtgga ataccagcgt
     8761 gtagcgctgt cagggtattg cctgctccgc catgatgaat gaaaccatct gcgccgttga
     8821 ggaagacacc catgggtatc cagtcaacca ggcgaacatt tgacggcagc gagcgcaaat
     8881 cggaccgcgc attggctgaa atatgcagga tgatctcggc atcgacctca ctggcagaat
     8941 ccataaccca ggcgattagg tcaaggccgt caaccatcgg tttgacagtg ccgagactca
     9001 ccagcagacg tttacgctcg ggccttctct cccaccatgg ttcccacact gcgccaccgt
     9061 tatacgggac gtactgcatc ggaataatgg gttcaccgtc gttttccaga atgctcatac
     9121 tcggcggtgt tacgtcgatc caggccatat cgcgtggtgt ggccccgaca ttgtggcgac
     9181 gataggcgtc cgtgagcgaa cgcgttacac ccctgatatg ccagggggta tggccgaagc
     9241 ctacggtttg catcacgacc ggtatatcat atttggcggc aatcagcggc ccgatgactc
     9301 cgagcggagg gtagatgatg aggtcaggcc gccagtgtcc ggcaaactcc accagatggt
     9361 cggccatttc ttcgctgaag aatgagaagt tgcccatttg ggttccgatg ttactttttt
     9421 tccgctgtgc ctcatggtgg cggtaaccag cttcagaatc cagaccgggg gcggcgtcaa
     9481 acaccaccaa cccggcctct gccgcttttt gtgcgaattg tcctccgctg gcgatcagta
     9541 cttcatgacc gttaacgcga aatgcctgcg ccagggacag cacaggatac aacagcccat
     9601 agagaggcgg gcctacaaac aaaatacgca tgagaaaatc cctctccgct tgacgatcta
     9661 aatcaggcag tcgacgcact catatgccag cagcaggaca cgacttttcg ttaaacctgc
     9721 tttaccagat gctgcgccat ttgtgcttca ccagcgttat ggacagatca gtcagagact
     9781 tttccgtgac atacacaggt gaacagggaa ctccgtatcc ccacttccat taacgcttac
     9841 gcgccattgt cctgttgaac agcccaaacg gcgtcggaca gctacgccgg gatgttctga
     9901 cgcatttgtg cagtgttaga ccgacaatga atctggaaag atccgtgact ttttagacac
     9961 acggcttgca tcatcctgat agtctcgcct tcgctggcct gtaagtaaaa tggccttgta
    10021 ctggcgatgc ttgttccctt tcaaccttac cagagataat tttaacgcaa atgctaatga
    10081 taataattac caatatcatc aggctattgc aacggtaatt aaaaacttaa tgaaaaggga
    10141 agcactcata ggactatctg gggtatttaa attgaaacct acgattccat tctctttcct
    10201 gtatgtaatc ctgacgcggg gaatcaaggg gagaacgtct atggctatct ttcaggaagt
    10261 gggactgcct aataactttt tgctgattgg atttattgct ggtgattatt atcttttatt
    10321 cgtatttccg actaagttgc tttacaacat tgagccaagc atgaatcact gctggagtca
    10381 ctttacccaa accactgcgg ccagtagttg cgacagttga aaaattgatc ttacccacgg
    10441 aatgtggaca cagtgctaag tgagattctg gttttcaaat tgttccgggc tgagaccgcc
    10501 gcacgcattg tggcgacgcc agcgattgta atcacactcg atataattaa cactgtcgtc
    10561 cgcattattt ctcggctggc aaagcgttct ccgtggatac agtccacttt caacgagtaa
    10621 aagaatcttt ccacacagtc attgtcatag cagcagccct ttgcactcat gctcccccgc
    10681 agattattac gtttcagcaa ggtctgataa tccgctgaac agtactggcc actacgtccg
    10741 gagtgaacaa tgacatcttc cggatgttta cgccgccaga gcgccatttg cagtgcatcg
    10801 caggaaagtt gtgtcgtcat acgcggcgac atcgaccagc caatgacggc tcgcgaccac
    10861 agatcaagca tcaccgccag atacagccag ccttcatccg tacgtaagta ggtgatgtca
    10921 cctgtccact tctggtttgg ccgctggcgt aaaaatcctg tttctgcaga ttctctgaca
    10981 cgggcaagcc gtgctcacgg taactgaccg gacagaactt gcgggtcgct ttcgctcgta
    11041 gtccctgacg acgcaggctg gcagcaattg ttttgatgtt gtattccggc aattcgtcag
    11101 caagacgagg cgcaccataa cgttgttttg cctcagtgaa tgctttacgg acagcagcat
    11161 cacagatgag ccggaactgc tggcgcgggc ttatttgatg gcgacgcagg agctcggcat
    11221 accagcggct gcgggcaacc cgaagtacac ggcacatggc tttgacgctg aactcagccc
    11281 gatgtttttc gataaagaca tacttcattt caggcgcttc gcgaagtatg tcgcggcctt
    11341 ttggagggag gccaattcct cgtcccgttc cgccagttgt cgcttaagac gggcaatttc
    11401 aacagacatg tcttgctcac gtttgcaaga ggaatgtgaa tttttcagct tgctacgcca
    11461 ggcataaagc tgagattaat acaggttgag ctcgcgtgca gcggcagcaa caccgatgcg
    11521 ttctgcaagc ttcagggctt cattgcgaaa ttcaggcgag tgttgcttac ggggcttttt
    11581 gctggctgat actggtttag tcatgagtca cctctgattg aaagtgtact cacttagtcc
    11641 cgtgtccact attggcgggt aggatcattg gtaggaatca taagcgtaat gacactggcg
    11701 gaagcagtta accaatcgaa gataaaaatg aaagcgaaag ccacctcgca ttgctcaatg
    11761 aattgaatca tatcgtcagg tgatcaccga tatttcttaa caaaaatata ataaaatagg
    11821 aac
//
LOCUS       DAZFRG010000149        11735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFRG010000149 DAZFRG010000000
VERSION     DAZFRG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.149"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..695
                     /gene="sdhC"
                     /locus_tag="ACHKUC_003263"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             291..695
                     /gene="sdhC"
                     /locus_tag="ACHKUC_003263"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6619471.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            689..1036
                     /gene="sdhD"
                     /locus_tag="ACHKUC_003264"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             689..1036
                     /gene="sdhD"
                     /locus_tag="ACHKUC_003264"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO6619472.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1036..2802
                     /gene="sdhA"
                     /locus_tag="ACHKUC_003265"
     CDS             1036..2802
                     /gene="sdhA"
                     /locus_tag="ACHKUC_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO6619473.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2818..3534
                     /gene="sdhB"
                     /locus_tag="ACHKUC_003266"
     CDS             2818..3534
                     /gene="sdhB"
                     /locus_tag="ACHKUC_003266"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO6619474.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3990..6791
                     /gene="sucA"
                     /locus_tag="ACHKUC_003267"
     CDS             3990..6791
                     /gene="sucA"
                     /locus_tag="ACHKUC_003267"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6619475.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6806..8023
                     /gene="odhB"
                     /locus_tag="ACHKUC_003268"
     CDS             6806..8023
                     /gene="odhB"
                     /locus_tag="ACHKUC_003268"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6619476.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8285..9451
                     /gene="sucC"
                     /locus_tag="ACHKUC_003269"
     CDS             8285..9451
                     /gene="sucC"
                     /locus_tag="ACHKUC_003269"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6619477.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9451..10320
                     /gene="sucD"
                     /locus_tag="ACHKUC_003270"
     CDS             9451..10320
                     /gene="sucD"
                     /locus_tag="ACHKUC_003270"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6619478.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            10650..11519
                     /locus_tag="ACHKUC_003271"
     CDS             10650..11519
                     /locus_tag="ACHKUC_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6619479.1"
                     /translation="MTLQRNGAQSVSGTTNASGSVNLGSTFADDQDALLIVKKEGYSN
                     LVVKCPCAGMTYAISPAMTSLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDA
                     NLDVDDTDSYGPETVTISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSS
                     LVRSYSVPAGKRGNIWTMFKLNPNGEFEDINSVTSANFNDTTLDVRDLATVIMPATGT
                     SASASPAMQNSGDTQLARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLG
                     LAYQKNGNIAEAI"
BASE COUNT         2857 a         2970 c         3215 g         2693 t
ORIGIN      
        1 cgcagatcac tgttcgggat aaaacccgac aaactatatg taggttaatt gtaatgattt
       61 tgtgaacagc ctatactgcc gccaggtctc cggaacaccc tgcaatcccg agccacccag
      121 cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg cagttataga
      181 aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt ctcctggtaa
      241 cagaaagtta acctctgtgc ccgtagtccc ctgggaataa taagaacagc atgtgggcgt
      301 tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta cagaccatcc
      361 ggttccccgt cacggcgata gcgtccattc tccatcgcgt ttccggtgtg atcacctttg
      421 ttgcagtggg catcctgctg tggcttctgg gtaccagcct ctcttcccct gaaggtttcg
      481 agcaagcttc cgcgattatg ggcagcttct ttgtcaaatt tatcatgtgg ggcatcctta
      541 ccgctctggc atatcacgtc gtcgtaggta ttcgccacat gatgatggat tttggctatc
      601 tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt attactgtcg
      661 tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat taggacgcaa
      721 tggcgtacat gatttcatcc tcgttcgtgc taccgctatc gtcctgacgc tctacatcat
      781 ttatatggtc ggttttttcg ctaccagtgg cgaactgaca tatgaagtct ggattggttt
      841 cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta tcttgatcca
      901 tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg ccttgcgcct
      961 gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg gattcgttgt
     1021 ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt gattggtgct
     1081 ggcggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gtggccagac ctgtgcgctg
     1141 ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggtgg tattaccgtt
     1201 gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac cgtaaaaggg
     1261 tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg gccggaagcg
     1321 attctggaac tggaacatat gggcctgccg ttctcgcgtc ttgatgatgg tcgtatctat
     1381 caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc acgtactgcg
     1441 gcggctgccg accgtaccgg tcacgcactg ttgcacacgc tttatcagca gaacctgaaa
     1501 aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaaaaa ccaggatggc
     1561 gcagtggtcg gttgtaccgc actgtgcatc gaaactggtg aagtggttta ctttaaagct
     1621 cgcgcgacag tgctggcgac tggcggggca gggcgtattt atcagtccac caccaacgcc
     1681 cacattaaca ctggcgacgg tgtcggcatg gctatccgtg caggcgtacc ggtacaggat
     1741 atggaaatgt ggcagttcca cccgaccggt attgccggtg cgggcgtact ggtcaccgaa
     1801 ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt tatggaacgt
     1861 tatgcgccga acgccaaaga cctggcgggc cgtgacgtgg tggcgcgttc catcatgatc
     1921 gaaatccgtg aaggtcgcgg ttgtgatggt ccgtgggggc cacacgcaaa actgaaactt
     1981 gaccatctgg ggaaagaagt tctcgaatcc cgtctgccgg gtattctgga actctcccgt
     2041 accttcgctc acgtcgatcc ggtgaaagag ccgattccgg ttatcccaac ctgtcactac
     2101 atgatgggcg gtattccgac caaagtgacc ggtcaggcgc tgactgtgaa tgagaaaggc
     2161 gaagatgtgg ttgttccggg gctgtttgcc gttggtgaaa tcgcttgtgt atcggtacat
     2221 ggcgctaacc gtctgggcgg caactcgctg ctggacctgg tcgtatttgg tcgtgcggca
     2281 ggtctacatc tgcaagagtc tatcgccgag cagggcgcac tgcgcgatgc cagcgagtct
     2341 gatgtagaag cgtctctgga tcgcctgaac cgctggaaca ataaccgtaa cggtgaagat
     2401 ccggtggcga tccgtaaagc actgcaagaa tgtatgcagc ataacttctc ggtcttccgt
     2461 gaaggtgatg cgatggcgaa agggcttgag cagttgaaag ttatccgcga gcgtctgaaa
     2521 aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga gtgcctggaa
     2581 ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt ccgcaccgaa
     2641 agccgtggcg cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa ctggctgtgc
     2701 cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa catggaaccg
     2761 aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga caggaaaatg
     2821 agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc gcgtatgcag
     2881 gattacaccc tggaagcgga agaaggtcgt gacatgatgc tgctggatgc gcttatccag
     2941 ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gccgtgaagg tgtgtgcggt
     3001 tccgacggtc tgaacatgaa cggtaagaat ggtctggcct gtattactcc gatttcggca
     3061 ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc ggtgatccgc
     3121 gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa gccttacctg
     3181 ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga gcagcgcgaa
     3241 aaactcgacg ggttgtatga atgtattctc tgcgcatgtt gttcaacctc ttgtccgtct
     3301 ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc atatcgtttc
     3361 ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag cgatgcattc
     3421 agtgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa ggggctgaac
     3481 ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc gtaaacccag
     3541 gcctgataag acgcgcaagc gtcgcatcag gcaaccagtg ccggatgcgg cgtgaacgcc
     3601 ttatccggcc tacaaaagaa atgcagaaac cgtaggcctg ataagacgca caagcgtcgc
     3661 atcaggcaac cagtgccgga tgcggcgtga acgccttatc cggcctacaa aagaaatgca
     3721 gaaaccgtag gcctgataag acgcgcaagc gtcgcatcag gcaaacagcg ccggatgcgg
     3781 cgtgaatgcc ttatccggcc tacaaaagaa atgcaggaaa tctttaaaaa ctgcccctga
     3841 cactaagaca gtttttaaag gttccttagc ggacgcagaa aacacgacaa gtccgcaaca
     3901 agtgaacccc ggcacgcaca tcagatatga aatgtgcgtg gtagtatcca cggcgaagta
     3961 agcataaaaa agatgcttaa gggatcacga tgcagaacag cgctttgaaa gcctggttgg
     4021 actcttctta cctctctggc gcaaaccaga gctggataga acagctctat gaagacttct
     4081 taaccgatcc tgactcggtt gacgctaact ggcgttcgac gttccagcag ttacctggta
     4141 cgggagtcaa accggatcaa ttccactctc aaacgcgtga atatttccgc cgcctggcga
     4201 aagacgcttc acgttactct tcaacgatct ccgaccctga caccaatgtg aagcaggtta
     4261 aagtcctgca gctcattaac gcataccgct tccgtggtca ccagcatgcg aatctcgatc
     4321 cgctgggact gtggcagcaa gataaagtgg ccgatctgga tccgtctttc cacgatctga
     4381 ccgaagcaga cttccaggag accttcaacg tcggttcatt tgccagcggc aaagaaacca
     4441 tgaaactcgg cgaactgctg gaagcactca agcaaaccta ctgcggcccg attggtgccg
     4501 agtatatgca cattaccagc actgaagaaa aacgctggat ccaacagcgt attgagtctg
     4561 gtcgcgcgac tttcaatagc gaagagaaaa aacgcttctt aagcgaactg accgccgctg
     4621 aaggccttga acgttacctc ggcgcaaaat tccctggcgc aaaacgcttc tcgctggaag
     4681 gcggtgacgc gttaatcccg atgcttaaag agatgatccg ccacgctggc aacagcggca
     4741 cccgcgaagt ggttctcgga atggcgcacc gtggtcgtct gaacgtgctg gtgaacgtgc
     4801 tgggtaaaaa accgcaagac ttgttcgacg agtttgccgg taaacataaa gaacacctcg
     4861 gcacgggcga cgtgaaatac cacatgggct tctcgtctga cttccagacc gatggcggcc
     4921 tggttcacct ggcgctggcg tttaacccgt ctcaccttga gattgtaagc ccggtcgtta
     4981 tcggttctgt tcgtgcccgt ctggacagac ttgatgagcc gagcagcaac aaagtgctgc
     5041 caatcaccat tcatggtgac gccgcagtga ccgggcaggg cgtagttcag gaaaccctga
     5101 acatgtcgaa agcgcgtggt tatgaagttg gcggtacggt acgtatcgtt atcaacaacc
     5161 aggttggctt caccacctct aacccgctgg atgcccgttc tacgccgtac tgtactgata
     5221 tcggcaagat ggtacaggca ccgattttcc acgttaatgc ggatgatccg gaagccgttg
     5281 cctttgtgac ccgtctggcg ctcgatttcc gtaacacctt taaacgtgat gtcttcatcg
     5341 atctggtgtg ctaccgccgt cacggccaca acgaagccga cgagccgagc gcaacccagc
     5401 cgctgatgta tcagaaaatc aaaaaacatc cgacgccgcg caaaatctac gctgacaagc
     5461 tggagcagga aaaagtcgcg acgctggaag atgccaccga gatggttaac ctgtaccgcg
     5521 atgcgctgga tgctggcgat tgcgttgtag cagagtggcg tccgatgaac atgcactctt
     5581 tcacctggtc gccgtacctc aaccacgaat gggacgaaga gtacccgaac aaagttgaga
     5641 tgaagcgcct gcaggaactg gctaaacgca tcagcacggt gccggaagcg gttgaaatgc
     5701 agtctcgcgt tgccaagatt tatggcgatc gccaggcaat ggctgccggt gagaaactgt
     5761 tcgactgggg cggcgcggaa aacctcgctt acgccacgct ggttgacgaa ggcattccgg
     5821 ttcgcctgtc gggtgaagac tccggtcgcg gtaccttctt ccaccgccac gcggtgatcc
     5881 acaaccagtc taacggttcc acttacacgc cgctgcaaca tatccataac ggccagggcg
     5941 cgttccgcgt ctgggactct gtactgtctg aagaagccgt actggcgttt gaatacggtt
     6001 atgccaccgc agaaccacgt accctgacca tctgggaagc acagttcggt gacttcgcca
     6061 acggtgcaca ggtggttatc gaccagttca tctcctctgg cgaacagaaa tggggccgga
     6121 tgtgtggcct ggtgatgttg ctgccgcacg gttacgaagg gcaggggccg gagcactcct
     6181 ccgcgcgtct ggaacgttat ctgcaacttt gcgctgagca aaacatgcag gtttgcgtac
     6241 cttctacccc ggcacaggtt taccacatgc tgcgtcgtca ggcgttgcgc gggatgcgtc
     6301 gtccactggt cgtgatgtcg ccgaaatccc tgctgcgtca tccgctggcg gtatccagcc
     6361 tcgaagaact ggcgaacggc accttcctgc cagccatcgg tgaaatcgac gagcttgatc
     6421 cgaagggcgt gaagcgcgta gtgatgtgtt ctggtaaggt ttattacgac ctgctggaac
     6481 aacgtcgtaa gaacaatcaa cacgatgtcg ccattgtgcg tatcgagcaa ctctacccgt
     6541 tcccgcataa agcgatgcag gaagtgttgc agcagtttgc tcacgtcaag gattttgtct
     6601 ggtgccagga agagccgctc aaccagggcg catggtactg cagccagcat catttccgtg
     6661 aagtgattcc gtttggggct tctctgcgtt atgcaggccg cccagcctcc gcctctccgg
     6721 cggtagggta tatgtccgtt caccagaaac agcaacaaga tctggttaat gacgcgctga
     6781 acgtcgaata aataaaggat acacaatgag tagcgtagat attctggtcc ctgacctgcc
     6841 tgaatccgta gccgatgcca ccgtcgcaac ctggcataaa aaacccggcg acgcagtcgt
     6901 acgtgatgaa gtgctggtag aaatcgaaac tgacaaagtg gtactggaag taccggcatc
     6961 agcagacggc attctggatg cggttctgga agatgaaggt acaacggtaa cgtctcgtca
     7021 gatccttggt cgcctgcgtg aaggcaacag cgcgggtaaa gaaaccagcg ccaaatctga
     7081 agagaaagcg tccactccgg cgcaacgcca gcaggcgtct ctggaagagc aaaacaacga
     7141 tgcgttaagc ccggcgatcc gtcgcctgct ggctgaacat aacctcgacg ccagcgccat
     7201 taaaggcacg ggtgtgggtg gtcgtctgac ccgtgaagat gtggaaaaac atctggcgaa
     7261 agccccggcg aaagagtctg ctccggcagc ggctgctccg gcggcgcaac cggccctggc
     7321 tgcacgtagc gaaaaacgtg tgccgatgac ccgcctgcgt aagcgtgtgg cagagcgtct
     7381 gctggaagcg aaaaactcca ccgccatgct gaccacgttc aacgaagtca acatgaagcc
     7441 gattatggat ctgcgtaagc agtacggtga agcgtttgaa aaacgccacg gcattcgtct
     7501 gggctttatg tccttctacg tgaaagcggt ggttgaagcc ctgaaacgtt acccggaagt
     7561 gaatgcgtct atcgacggcg atgatgtggt ctaccacaac tatttcgacg tcagtatggc
     7621 ggtttctacg ccgcgcggtc tggtgacgcc ggttctgcgt gatgtcgata ccctcggcat
     7681 ggcagacatc gagaagaaaa tcaaagagct ggctgttaaa ggccgtgacg gcaagctaac
     7741 ggttgaagat ctgaccggtg gtaacttcac catcaccaac ggtggtgtgt tcggttccct
     7801 gatgtctacg cctatcatca acccgccgca gagcgcaatt ctgggtatgc acgctatcaa
     7861 agatcgtccg atggcagtga atggtcaggt tgagatcctg cctatgatgt acctggcgct
     7921 gtcctacgat caccgtctga tcgatggtcg cgaatccgtg ggcttcctgg taacgatcaa
     7981 agagttgctg gaagatccga cgcgtctgct gctggacgtt tagtagttta agcttcacct
     8041 gcactgtaga ccggataaga caatattgac ttctccggca atcgaagcct gatgcgacgc
     8101 taatgcgtct tatcaggcct acggtgatca ccaatgtagg tcggataagg cgcttacgcc
     8161 gcatccgaca agcgatgtct gatgcgacat caggcctacg ggtgaccggc agtacccgga
     8221 cgcgatacgt catattcggt ctacggttta aaagataacg attactgaag gatggacaga
     8281 acacatgaac ttacatgaat atcaggcaaa acaacttttt gcccgctatg gcttaccagc
     8341 accggtgggt tatgcctgta ctactccgcg cgaagcagaa gaagccgctt caaaaatcgg
     8401 tgccggtccg tgggtagtga aatgtcaggt tcacgctggt ggccgcggta aagcgggcgg
     8461 tgtgaaagtt gtaaacagca aagaagacat ccgtgctttt gcagaaaact ggctgggcaa
     8521 gcgtctggta acgtatcaaa cagatgctaa tggccaaccg gttaaccaga ttctggttga
     8581 agcagcgacc gatatcgcta aagagctgta tctcggtgcc gttgttgacc gtagttcccg
     8641 tcgcgtggtc tttatggcct ccaccgaagg cggcgtggaa atcgaaaaag tggcggaaga
     8701 aactccgcac ctgatccaca aagttgcgct tgatccgctg actggcccga tgccgtatca
     8761 gggacgcgaa ctggcgttta aactgggtct ggaaggtaaa ctggttcagc agttcaccaa
     8821 aatcttcatg ggcctggcga ccattttcct ggagcgcgac cttgcgctta tcgaaatcaa
     8881 cccgctggtt attaccaaac agggcgatct gatttgcctc gacggcaaac tgggcgctga
     8941 tggcaacgca ctgttccgcc agcctgatct gcgtgaaatg cgtgaccagt cgcaggaaga
     9001 tccgcgtgaa gcacaggctg cacagtggga actgaactac gttgcgctgg acggtaacat
     9061 cggttgtatg gttaacggcg caggtctggc gatgggtacg atggacatcg ttaaactgca
     9121 cggcggcgaa ccggctaact tcctggacgt tggcggcggc gcaaccaaag aacgtgtaac
     9181 cgaagcgttc aaaatcatcc tctctgacga caaagtgaaa gccgttctgg ttaacatctt
     9241 cggcggtatc gttcgttgcg acctgatcgc tgacggtatc atcggcgcgg tagcagaagt
     9301 gggtgttaac gtaccggtcg tggtacgtct ggaaggtaac aacgccgaac tcggtgcgaa
     9361 gaaactggct gacagcggcc tgaatattat tgcagcaaaa ggtctgacgg atgcagctca
     9421 gcaggttgtt gccgcagtgg aggggaaata atgtccattt taattgataa aaacaccaag
     9481 gttatctgcc agggctttac cggtagccag gggactttcc actcagaaca ggccattgca
     9541 tacggcacta aaatggttgg cggcgtaacc ccaggtaaag gcggtactac ccacctcggc
     9601 ctgccggtgt tcaacaccgt gcgtgaagct gttgctgcta ctggcgctac cgcctctgtt
     9661 atctacgtac cagcaccgtt ctgcaaagac tccattctgg aagctatcga tgcaggcatc
     9721 aaactgatta tcaccatcac tgaaggcatc ccgacgctgg atatgctgac ggtgaaagtg
     9781 aaactggatg aagcaggcgt tcgtatgatc ggcccgaact gcccaggcgt tatcaccccg
     9841 ggggaatgca aaatcggtat ccagccgggt cacattcaca aaccgggtaa agtgggtatc
     9901 gtttcccgtt ccggtacgct gacctatgaa gcggttaaac agaccacgga ttacggtttc
     9961 ggtcagtcga cctgtgttgg tatcggcggc gacccgatcc ctggctctaa ctttatcgat
    10021 atcctcgaaa tgttcgaaaa agatccgcag accgaagcga tcgtgatgat cggtgagatc
    10081 ggcggtagcg ctgaagaaga agcagcggcg tacatcaagg agcacgttac caagccagtt
    10141 gtgggttaca tcgctggtgt gactgcgccg aaaggcaaac gtatgggtca cgcaggtgcg
    10201 atcattgccg gtggtaaagg gactgctgat gagaaattcg ctgctctgga agccgcaggc
    10261 gtgaaaaccg ttcgcagcct ggcggatatc ggtgaagcac tgaaaactgt tctgaaataa
    10321 atatctgtaa taagaaatag ccctcgccgc ttccctctac aggaggggcg aagggctgtc
    10381 ggtttcgaca tggttggcca tcttatgatg gcctttttta ttcgggcact aatctgaaaa
    10441 gtttgcccac tgcttttttc ccatcttctg cgcaataatg cccggcaaaa tattccctct
    10501 ccgttctgta aaagcattta tacaagaaga aaattgttct gccagttgct ctggccctga
    10561 tgggtataaa ttcggacgtt tatgcggccg atgtaaatat cgatatttta agtgcgacag
    10621 tgaaagataa acgcattgag ggagcatcga tgacgctgca acgcaatggc gcacaatctg
    10681 tttctggaac cacaaatgca tcgggcagcg ttaatctggg ttctacattt gctgatgatc
    10741 aagatgcttt attgatcgtc aaaaaagagg gctattctaa tctggtggtc aaatgtccgt
    10801 gtgctggcat gacttatgcg attagtccgg caatgactag tctggatggc atgcgtgtag
    10861 ttttgagctg gggcgaaaag ccttttgatc ttgattcaca tttgatcttc cctggcggac
    10921 atatctattt tgatagtaaa gaggggactg atgctaatct ggatgttgat gatacggata
    10981 gctatggacc agaaactgtc actatcagca aaaagcattt tggcgaaagt tacatctatg
    11041 ccgtgcagga ctatagcaat aaagggttgc ccaacagtaa ttatctctct gccagtaagg
    11101 caaaagtgtt tgtttatgta gggagctccc tggtgcgtag ttatagtgtc cctgctggaa
    11161 aacgagggaa catctggacc atgtttaaac tcaatccaaa cggcgagttt gaagatatta
    11221 actctgttac cagcgctaat tttaacgaca ccacattaga cgtcagggat ttagccactg
    11281 tgataatgcc tgcgacgggc acttcagctt cggcttcgcc tgcgatgcaa aacagtggag
    11341 atactcaact ggcaagaaaa tataatcgcg aaggtgaagc tgtttataaa accggtcaac
    11401 ttgagcaagc cattcaactc ttccaacagg caacagaatt agacggcaat tacggacagg
    11461 catttagtaa cctggggctg gcgtatcaga aaaatggcaa catagcggaa gcaatttagg
    11521 caaatcgtaa agcgatctcg ttagcctgtg gagcaaatgc ggcgacaacg cgcgctaact
    11581 cctattataa cattgccaaa atttatggaa ctgctggaca ggacgctgat gcattgcagc
    11641 attatcaact cgctcttcac gatgccattt tataacaatg aaagtggaat catattttta
    11701 aattttatat tcttgcttgt ttttctggtg aagtt
//
LOCUS       DAZFRG010000150        11666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFRG010000150 DAZFRG010000000
VERSION     DAZFRG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.150"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..332)
                     /gene="yehE"
                     /locus_tag="ACHKUC_003272"
     CDS             complement(51..332)
                     /gene="yehE"
                     /locus_tag="ACHKUC_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO6619480.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(595..1704)
                     /gene="apbC"
                     /locus_tag="ACHKUC_003273"
     CDS             complement(595..1704)
                     /gene="apbC"
                     /locus_tag="ACHKUC_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO6619481.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     DSEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1836..3869
                     /gene="metG"
                     /locus_tag="ACHKUC_003274"
     CDS             1836..3869
                     /gene="metG"
                     /locus_tag="ACHKUC_003274"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO6619482.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4010..7798
                     /locus_tag="ACHKUC_003275"
     CDS             4010..7798
                     /locus_tag="ACHKUC_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO6619483.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTNGQNQVKSFAD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGAPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIANIAENRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTIS
                     FDETLKPFVRDASGCRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWTGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMINPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQILSKLWLWEGKAESYGWGSNSTQEAQFSVLDTITASELINDIEALFE
                     "
     gene            7808..11440
                     /gene="yehI"
                     /locus_tag="ACHKUC_003276"
     CDS             7808..11440
                     /gene="yehI"
                     /locus_tag="ACHKUC_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO6619484.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMATLACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDSKAVKELARYWSQDIFSDREASYMSHENHFGYTACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLSTQAQAVVNSYLSAPLKQVIDSTDNSCLPEILVSPPWRSKKKMTAPRLDLASL
                     ELTPQVYWQLGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILHGWNSPEAQLAEQALRSGHVEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARELTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGLRQFTVRFDENLNPVIYDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFHSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11501..>11666
                     /locus_tag="ACHKUC_003277"
     CDS             11501..>11666
                     /locus_tag="ACHKUC_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619485.1"
                     /translation="MIVQKELVAIYDYEIPAPEDPFSFRLEIHKFSELFTGSVYRLER
                     FRLHPTFNDAD"
BASE COUNT         3046 a         2994 c         2839 g         2787 t
ORIGIN      
        1 catgtaaatg atttaagctt tctaaatccg attaaaacca gggtggcaaa ttaatcgtag
       61 cgatttaaca cgattcgacg tttgctatcg ctatctgcag caatcacttc cgtcgttaca
      121 cctttaactg gcgaagaaac ctgatgacgg gtatcaacat tatacgttaa ctgaccacaa
      181 tgctgttgag gttgaccatt aaccatagcc atactgcatg ttgggggact gatcctacca
      241 ttaatcgtta atgtggcagt ttctgaagag aacgcgggag aagccaatcc ataggcaaga
      301 aaaataattc ctgacagcca gtacttattc attgaacgtt atccctgtag taaaggttat
      361 gcctggaaag aatgagtgca taacaaacta tagctgtaca tccactacac agccacgaag
      421 gatgataatg aagcatcgcc ggtacgatca atcgactttg tagagtttcg gacgaaggtc
      481 cgcagaatat tcgcagcatt aaataagtgt tcattaatgc ggcaaaaaca aatacaccgc
      541 atcaatgtaa catctctgcg gtgtatgaag aataatcaga atggttaata cgcgttagac
      601 cgcgcggaag gaaatctcgc ctggaatgac ttcaccctgc cagtagagct gagctgcaac
      661 gcggtcagcc aactggcggt agatagcggt aaattcgcta tcaggacggc tgataaccgt
      721 tggcgtgcct ttatcgagat cctcacgtaa ggagatgtgt aacggcatct gccccagcag
      781 ctgtgtgtgg tatttctcgg ccagtttctc tgcaccaccg gtaccgaaaa tcggctcatg
      841 gtgaccacag ttactgcaaa tatgcacact catattctct acaataccga gcaccggaac
      901 ttcgactttc tcgaacatca caatgccttt tttcgcatcg atcaacgcga tgtcctgcgg
      961 ggtggtgacc acgactgcac cggttactgg aatgttttgt gccagtgtca attgaatgtc
     1021 accggtacct ggcggcatat caagcaccag atagtcgaga tccggccaca aggtttcctg
     1081 caacatttgc attaacgcct tgctcgccat tgggccacgc cacaccatcg cattgtcgtc
     1141 tgtcaccaga taaccaattg aattagttgc caggccatga gacataatcg gtgccatatg
     1201 ggtaccgtcc ggcgaagttg gacgctgatt ttctgcgccc agcatggtag ggattgatgg
     1261 gccgtagata tcggcgtcca gaatgccgac tttcgcccct tccgccgcca gtgccaacgc
     1321 caggtttacc gccgtggagg atttacccac gccaccttta ccggaactga ccgcgataat
     1381 gttcttcacg ccgttaattc ccggctgatt cttcacgcgt ttcagagtgg ctatgttatg
     1441 cgacagtttc cagtcgatag ctttcgctcc ggtgatgcgc agcagttcgg cgctacattg
     1501 ctcttttaac tcttcgaacg cgctatgcca gacgaatggc ataaccagtt ccacatgcag
     1561 cgtgtcgtcc atccaggcga catggtgcaa cgctttcagc gttgtgaggt tatgcttcag
     1621 ggttgggtgc tgaaaattgg ccagtgtccc ggcgaccatt gcgcgcaagg cttctggtga
     1681 tttggcctgg gattgttcgt tcatcccgac tccttttttg tatagataaa ccatcagctg
     1741 atagtttacc tgaagaatat agagaagtac ttacttaaca ttttcccatt tggtactatc
     1801 taagcccttt tcactattaa gaagtaatgc ctactatgac tcaagtcgcg aagaaaattc
     1861 tggtgacgtg cgcactgccg tacgctaacg gctcaatcca cctcggccat atgctggagc
     1921 acatccaggc tgatgtctgg gtccgttacc agcgaatgcg cggccacgag gtcaacttca
     1981 tctgcgccga cgatgcccac ggcacgccga tcatgcttaa agctcagcag cttggtatca
     2041 ccccggagca gatgattggc gaaatgagtc aggaacatca gactgatttc gcaggcttta
     2101 acatcagcta tgacaactat cactcgacgc acagcgaaga gaatcgccag ttgtcagaac
     2161 ttatctactc tcgtctgaaa gaaaacggtt ttattaaaaa ccgcaccatc tctcagctgt
     2221 acgatccgga aaaaggcatg ttcctgccgg accgttttgt aaaaggcacc tgcccgaaat
     2281 gtaaatcccc ggatcaatac ggcgataact gcgaagtctg cggcgcgacc tacagcccga
     2341 ctgaactgat cgagccgaaa tcggtggttt ctggtgctac cccggtaatg cgtgattctg
     2401 aacacttctt ctttgatctg ccctctttca gcgaaatgct gcaggcatgg acccgcagcg
     2461 gtgcgttgca ggagcaggtg gcaaataaaa tgcaggagtg gtttgaatct ggcctgcaac
     2521 agtgggatat ctcccgcgac gcgccttatt tcggttttga aattccgaac gcgccaggca
     2581 aatatttcta tgtctggctg gacgcaccga ttggctatat gggctctttc aagaatctgt
     2641 gtgacaagcg cggcgacagc gtaagcttcg ataaatactg gaagaaagat tccactgccg
     2701 agctgtatca cttcatcggt aaagatatcg tttacttcca cagcctgttc tggcctgcca
     2761 tgctggaagg cagcaacttc cgcaagccga ccaacctgtt tgttcacggc tatgtgacgg
     2821 tgaacggcgc gaaaatgtcc aagtctcgcg gcacctttat taaagccagc acctggctga
     2881 atcattttga cgccgacagc ctgcgctact actacaccgc caaactgtct tcgcgcattg
     2941 atgatatcga tctcaacctg gaagatttcg ttcagcgcgt gaatgccgat atcgtgaaca
     3001 aagtggttaa cctggcgtcc cgtaacgctg gctttatcaa taagcgtttt gacggtgtgc
     3061 tggcaagcga gctggctgac ccgcagttgt acaaaacctt cactgatgcc gctgaagtga
     3121 ttggtgaagc gtgggaaagc cgcgaatttg gtaaagctat ccgtgaaatc atggcgctgg
     3181 ctgacctggc taaccgctat gtcgatgaac aggctccgtg ggtggtggcg aaacaggaag
     3241 gccgcgatgc cgacctgcag gcaatttgct cgatgggcat caacctgttc cgcgtgctga
     3301 tgacttacct gaaaccggta ctgccgaaac tgaccgagcg cgcagaagca ttcctcaata
     3361 cagaactgac ctgggatggt atccagcaac cgctgttggg ccataaagtg aatccattca
     3421 aggctctgta taaccgcatc gatatgaaac aggttgaagc actcgtggaa gcatcaaaag
     3481 aggaagtaaa agccgctgcc gcgccagtaa ctggcccact ggcagatgat ccgattcagg
     3541 aaaccatcac ctttgacgac ttcgccaaag ttgacctgcg cgtggcgttg attgaaaacg
     3601 cagagtttgt agaaggttct gacaaactgc tgcgcctgac gctggatctc ggcggtgaaa
     3661 aacgcaatgt cttctccggc attcgttctg cttacccgga tccgcaagca ctaattggtc
     3721 gtcacaccat tatggtggct aacctggcac cacgtaaaat gcgcttcggt atctccgaag
     3781 gcatggtaat ggctgctggt cctggcggga aagatatctt cctgctaagc ccggatgccg
     3841 gtgctaaacc gggtcatcag gtgaaataat cccccttcaa ggcgctgcat cgacagcgcc
     3901 ttttctttat aaattcctaa agttgttctc ttgcgatttt gtctctctct aacccgcata
     3961 aatactggta gcatctgcat tcaactggat aaaattacag ggatgcagaa tgagacactt
     4021 tatctatcag gacgaaaaat cacataaatt ctgggcggtg gagcagcaag gcaacgagtt
     4081 acatatcagt tggggcaaag tcggcactaa cgggcaaaac caggtaaaaa gctttgctga
     4141 tgctgcggcg gcggaaaaag cggaactgaa gctgattgcg gagaaggtga agaagggata
     4201 tgtggagcaa gcgaaggata attctttgca accttcccaa acggtaacgg actctctcaa
     4261 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
     4321 ggctgacaaa aatacagatg ctgttttacc atggctggcg aaagatattg ctgtcgtttt
     4381 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ctcctgttca
     4441 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agtgtatcgc aacgggataa
     4501 taaaacagcc acatttgact tcagcgcctg ttctttagaa tggcaaaaca ccgtcgcaca
     4561 ggcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcacccg taatggctgt
     4621 actcacggca cttgaaatga aatgcacaag atataaagag cgtgaggatg ttatggatca
     4681 gattattcag gaaggcggtc tggaatatgc tactgatgta ataatacatc ttcaacaaat
     4741 atctattgaa tgggattatg tcaacaataa tattgttttc ctgtcgtctg gcatttcacc
     4801 tgattacttg cagcaatatt ccagctttga attacgccta cgtaaacatt tatcactggc
     4861 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
     4921 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
     4981 aatttcccag cgtctactgg ggcaaaagaa attaccctcg cttgagtggc taaaaatagt
     5041 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
     5101 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttac
     5161 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     5221 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     5281 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc
     5341 ggaacttcta gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgcttat
     5401 ctcgcatccg acactagcag aacaggtcat tccctggctt tcgacacccg cagttgcagt
     5461 gctgaaatca tgtcagcaac aactgacaca gccctcaaac catgccagcg ccgatctact
     5521 gccggccata gtagtctccc caccatggct ttcgaaaaag aaaaaatcgc cgattccggt
     5581 gctggattta gcaccgctca acctcgaatc catatgcaca ataaccgaca cagaagctaa
     5641 agagtttcaa actcattggg actgggaacc gcataagcca agtgaaggtg ctaaaaattt
     5701 cctttactcg ctggggtatc gccgctggga ttttgacaca tataaatata tcggcgcttc
     5761 tgactcggca attgacgcat gggaacgtga ggacttcgct acactcatcc aaatgtttaa
     5821 agcccaccat gcaccgtatc agggggagtg gcatctcaac tcacttccct ttttacctat
     5881 gcaaaaagcg attaagctat gggaatttct cagcaaagag ccgcataccg cgataaaacc
     5941 agtcatgctt tatttgcgac tggcaggtat gagtggtttt ctacactcgt tttcacgcta
     6001 ccctcaagaa ggttttgcgg ttgctaatta ttttgccgca acggaactgg ctcctgccgt
     6061 cgcccgcgcc ttcaacaaac tcaaaacatt acgacaagac gccagtagct ggctgctgaa
     6121 atatccggaa cacgccatta ccggcctgct gcctgcagcg ctcggcaaag ccagtgaagc
     6181 acaggacaac gcccgtgctg ccttgcgtat gcttaccgaa aacggtcatc agccattgct
     6241 gcaagaaatc gcccggcgtt ataaccagcc ggaagtaacc gatgcggtga acgctctact
     6301 tgcgctcgat cccttagata atcacccgac aaaaatcccc actcttccga ccttttatca
     6361 gccatcgctc tggacgcgcc cgttattaaa agcaaacgcc caatcactgc ctgatagcgc
     6421 actcctccac ctcggtgaaa tgctccgctt ccctcaggaa gaggcactgt atccgggatt
     6481 attgcaggtg aaagatgcct gtaccaccga ctcactggct gaatttgcct gggatctgtt
     6541 taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca ctgcgttagg
     6601 cgttctcggt aacgatgaca ccgcccgcaa actgacgcct ttgattcgcg cctggcctgg
     6661 tgaatcccag cataaacgcg ccaccgtcgg gttggatatt ctcgctgcta tcggtagtga
     6721 tatcgccctg atgcagctta acggcatcgc ccagaaactg aaattcaaag cattacagga
     6781 gcgggcgaaa gaaaaaattg ccaacattgc cgagaaccgc gaactcacgg tagcggagct
     6841 tgaagatcgg ttagcaccgg atctcggtct ggatgataac ggttcgttgc tgctggattt
     6901 cggcccacgc cagttcacca tcagctttga tgaaacctta aaaccgtttg tgcgtgacgc
     6961 ttccggctgt cgtctgaaag acctgcccaa accaaacaaa agcgatgatg aatcacaggc
     7021 gaacgatgcg gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg tcgccgccca
     7081 gcaggtagca aggctggaat ccgccatgtg cctgcgtcgc cgctggtcac cggaaaactt
     7141 tcagctcttc ctggttgagc atccgctggt ccgccactta acccgccgtc tgatttgggg
     7201 cgtttatagc gccgaaaacc agctactggc ttgctttcgc gtagcggaag ataacagcta
     7261 cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta ttggtattcc
     7321 tcacgttctg gaaatatcac cgacggatgc tgccgccttc gggcagcttt ttgccgacta
     7381 cgaactgtta ccaccattcc gccagcttga tcgtaacagt tacgcgctga cagaagccga
     7441 gcgcaacgcc agcgaactga cacgctggac aggcagaaaa tgcccgagtg gtcgggtaat
     7501 ggggctggcg aataaaggct ggataaaggg caccccgcag gatgctggct ggatcggctg
     7561 gatgatcaac cccctgggcc gctggtcgtt aatcatggaa atcgatgaag gttttgcggt
     7621 aggcatgtcg ccagccgaac tgagtgccga gcaaatctta agcaagctgt ggctatggga
     7681 aggcaaagca gaaagctatg gctgggggag taattcaaca caggaagcgc agttctccgt
     7741 actcgatacc atcaccgcca gcgagctaat taacgatatt gaagccctgt ttgaataagg
     7801 aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg aacttaaata
     7861 taaaaaaggc aaaacaccac tcagccatcg caattggcca ggcgaaccgg tacctgttat
     7921 cactgaaaac atcattcaga cactgggtga tgaattgcta caaaaagcag agaagaaaaa
     7981 aaacattgtc tggcgttatg aaaacttttc actggagtgg cagtccgcca tcacgcaggc
     8041 catcaacttg atcggcgaac acaaaccctc aatcacggcc cggacaatgg cgacgctagc
     8101 ctgtatcgcg caaaatgata gccaacagtt gctcgacgaa atcgtccaac aagaggggct
     8161 ggaatatgca actgaggtgg tgattgcacg ccagtttatt gtacggtgtt atgagagtga
     8221 tcctctggta gtaacattgc aatatcagaa cgaggactat ggctatggtt atcgctcaga
     8281 aacctataac gaattcgatc tccgattgcg taagcatctc tctctggcag aggaaagctg
     8341 ctggcagcgt tgcgccgaca aactcattgc cgcactgcca ggaataacca aagttcgccg
     8401 cccttttatt gcgctgaacc tcccggaaaa acccgaaatc gccaacgagc tggtaagcct
     8461 tgaatgttcg caaactcatt ttcgctcaaa agagtggtta aaagttgttg ctgatgactc
     8521 taaggcggtg aaagaactcg cacgttactg gagccaggat atatttagcg atcgagaagc
     8581 cagctacatg tcgcatgaaa accacttcgg ttacacggcc tgcgctgccc ttttgcgcga
     8641 acaaggactg gcagccgttc cgcgcctcgc gatgtatgca cataaagaag attgcggcag
     8701 tctgctggta caaattaacc atccgcaagt catccgcacc ttgctactgg tggctgataa
     8761 aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc atgcgacgct
     8821 cgccgcactg gcagaactgc tggcgttaaa agccccccct gcgcgccctg gttatcccat
     8881 catcgaagac caaaagctgc ctgcacagca aaaagcacgt gatgaatact ggcatacgct
     8941 gttacaaacg ctgatggcat cgcagccaca actggcagaa gaggtgatgc cgtgcttaag
     9001 tacacaagcc caggcagtgg tgaatagtta tttatcggca ccgctaaaac aggttattga
     9061 tagcaccgat aatagctgtc tgcctgaaat cctcgtttca ccaccgtggc gtagtaagaa
     9121 aaaaatgaca gctccacgtc ttgatttggc atcgctcgaa ttaactccgc aagtttactg
     9181 gcaactaggc gaacgagaga gacttgccgc cacagagtct gcccgttatt tcagcacggg
     9241 atcttttacg gaacgcatgg aacgaaaaag tggtcgagtt gtactacagg aactgggttt
     9301 tggggatgat gtatggctgt ttttgaatta tatactcccc ggaaaactgg atgctgcacg
     9361 caattcactc attgttcagt ggcattacta ccaggggcga gttgaagaga tcctgcatgg
     9421 ctggaactcc ccggaagcac aattagcaga acaggcgctt cgcagcggtc acgtagaagc
     9481 gttaattaac atatgggaaa atgacaacta ctcacgttat cgtccggaaa agagtgtctg
     9541 gaacctgtat ttattggcac agttgccgcg tgagatggct ttgactttct ggctgcgtat
     9601 caatgagaaa aagcatctgt tcgcgggtga ggactatttt ctcagtatcc tcgggttgga
     9661 tacattacct ggcctgttat tggctttttc acatcatcca aaagaaacat ttccgttaat
     9721 tttaaatttc ggcgcaacag aactggccct gcccgttgcc cgcgtctggc gacgtttcgc
     9781 ggcgcagcgt gatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc
     9841 acttatccct cttgtcttta caaaacccag cgataatagc gaagctgcat tacttgccct
     9901 gcgtttactg tatgaacaag ggcatggcga attactgcaa accgtggcaa accgctggca
     9961 gcgtactgat gtatggcctg ccctggagca attacttaaa ctgggtccaa tagaaattta
    10021 cccggcacgt attccaaaag ctcctgattt ctggcatccg gcaatgtggt ccagaccgcg
    10081 ccttatcaag aataatcagc ctgttaccga tgacgctctg gaaattatcg gcgaaatgct
    10141 gcgctttacc cagggcggac gtttttatag cggactggaa cagctgaaaa cgttttgcca
    10201 gccgcaaacg ctggcagctt ttgcctggga tcttttcacc gcgtggcaac aagctggtgc
    10261 ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc
    10321 tcgggagctg acgacacaga tcctcgcctg gccacaagaa ggcaaatctg cccgtgctgt
    10381 cagtggactg aacatcctta ccctgatgaa taatgatatg gcgctgatac agctgcacca
    10441 tatatcccaa cgggcgaaat cccgctcctt acgtgataac gcggcggaat ttcttcaggt
    10501 ggtcgcagaa aatcgcgggc taagccagga agagttagcg gacagattag tcccaacatt
    10561 gggccttgat gatccgcagg cgttgagttt tgattttggt ctccggcagt ttaccgttcg
    10621 ctttgatgaa aacctcaacc cggttatcta cgatcaacaa aacgttcgcc agaaaagtgt
    10681 tccccggttg cgcgccgatg acgatcaact gaaagcgccc gaggcactgg cccgactaaa
    10741 agggctaaaa aaagatgcca ctcaggtgag caaaaacctg ctcccacgtc ttgaagcagc
    10801 cctacgtatc acccgccgct ggtcgcttgc agattttcat tctctgtttg ttaatcatcc
    10861 ctttacccgc ctggttaccc agcgattaat atggggggtt tatccggcaa atgaaccgcg
    10921 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg agcaagatga
    10981 gccaattgac ctgcctgccg atgctctgat tggcattgcc cacccgttag aaatggcagc
    11041 agaaatgcgc agtgaatttg cacagctttt tgccgattac gaaattatgc cgccttttcg
    11101 ccagttggcg cggcgcacgg tgctgctcac acctgacgag tcagccagta acagcctgac
    11161 tcgctgggaa ggtaaatccg ctaccgttgg gcaacttatg ggaatgcgat acaaaggctg
    11221 ggagtcaggc tatgaggacg catttgtcta tgacttaggt gcgtaccggc tggtccttaa
    11281 gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgctaatga gcttccattc
    11341 tcttcgggtt taccgtgaca ataaatccgt cacttttgcc gaacttgatg tgtttgattt
    11401 gagtgaagcg ttaagcgcac ccgacgttat tttccattaa aacacagcgg gcagtgatgc
    11461 aactgcccgt tatcgacacg acttacctcc gggaaaagcg atgatcgtac aaaaagagct
    11521 ggtcgctatt tacgattatg agattcctgc tcctgaagat ccgttttcct tcagacttga
    11581 gatccataaa ttctctgaat tatttacagg ttccgtctat cgactggaac gattccggct
    11641 acatccaaca tttaatgatg ccgact
//
LOCUS       DAZFRG010000151        11625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFRG010000151 DAZFRG010000000
VERSION     DAZFRG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.151"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..968
                     /gene="yabI"
                     /locus_tag="ACHKUC_003278"
     CDS             204..968
                     /gene="yabI"
                     /locus_tag="ACHKUC_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6619486.1"
                     /translation="MQALLEHFITQSTVYSLMAVLLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPAFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1082..1780)
                     /gene="thiQ"
                     /locus_tag="ACHKUC_003279"
     CDS             complement(1082..1780)
                     /gene="thiQ"
                     /locus_tag="ACHKUC_003279"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO6619487.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(1764..3374)
                     /gene="thiP"
                     /locus_tag="ACHKUC_003280"
     CDS             complement(1764..3374)
                     /gene="thiP"
                     /locus_tag="ACHKUC_003280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO6619488.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQDDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIENIPGRNVKTD"
     gene            complement(3350..4333)
                     /gene="thiB"
                     /locus_tag="ACHKUC_003281"
     CDS             complement(3350..4333)
                     /gene="thiB"
                     /locus_tag="ACHKUC_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO6619489.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESVQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYATANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(4364..4463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4497..6152)
                     /gene="sgrR"
                     /locus_tag="ACHKUC_003282"
     CDS             complement(4497..6152)
                     /gene="sgrR"
                     /locus_tag="ACHKUC_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO6619490.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     STMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTMKIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            6241..6372
                     /gene="sgrT"
                     /locus_tag="ACHKUC_003283"
     CDS             6241..6372
                     /gene="sgrT"
                     /locus_tag="ACHKUC_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO6619491.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(6480..7085)
                     /gene="leuD"
                     /locus_tag="ACHKUC_003284"
     CDS             complement(6480..7085)
                     /gene="leuD"
                     /locus_tag="ACHKUC_003284"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO6619492.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(7096..8496)
                     /gene="leuC"
                     /locus_tag="ACHKUC_003285"
     CDS             complement(7096..8496)
                     /gene="leuC"
                     /locus_tag="ACHKUC_003285"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO6619493.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRSVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(8499..9590)
                     /gene="leuB"
                     /locus_tag="ACHKUC_003286"
     CDS             complement(8499..9590)
                     /gene="leuB"
                     /locus_tag="ACHKUC_003286"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO6619494.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(9590..11161)
                     /gene="leuA"
                     /locus_tag="ACHKUC_003287"
     CDS             complement(9590..11161)
                     /gene="leuA"
                     /locus_tag="ACHKUC_003287"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO6619495.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(11253..11339)
                     /gene="leuL"
                     /locus_tag="ACHKUC_003288"
     CDS             complement(11253..11339)
                     /gene="leuL"
                     /locus_tag="ACHKUC_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO6619496.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         2577 a         3130 c         3143 g         2775 t
ORIGIN      
        1 tgagcaccgt gattctctgc aggtggaata ttttgagact atcgaatgat aggcagaggg
       61 tgtcttttgt gccggttgtg aagaaaaagt gaatgatgta gccgtcaagt tgtcataatt
      121 ggtaacgaat cagataattg acggcttgac ggagtagcat agggtttgca gaatccctgc
      181 ttcgtccatt tgacaggcac attatgcaag cattgctgga acactttatt acccaatcta
      241 ccgtgtattc attgatggcg gtgttattgg tggcctttct ggagtcgctg gcgctggtcg
      301 gtttgattct acccggtacg gtgctgatgg cggggctggg agcgctgatt ggtagcggtg
      361 agttaagttt ctggtatgcc tggctggcgg ggattattgg ctgcctgctg ggcgactgga
      421 tctccttctg gctgggctgg cgttttaaaa agccgttgca tcgctggtca tttctgaaga
      481 agaacaaagc attactcgat aaaaccgaac atgcgctgca tcagcacagc atgttcacca
      541 ttctggtcgg tcgttttgtc ggcccgacgc gtccgctggt gccaatggtg gcgggaatgc
      601 tggatctccc ggtggcaaaa tttattacgc cgaatattat cggctgcttg ctgtggccag
      661 cgttttactt cctgccgggg attctggcgg gtgcggcgat cgatattcct gccgggatgc
      721 agagcggtga gtttaaatgg ttgctgctgg caacagcggt gtttttgtgg gttggaggct
      781 ggctgtgctg gcggttatgg cgcagcggta aagcgactga ccgtttgagt cattatttgt
      841 cccgcggtcg tttgttgtgg ctgacgccgt tgatttctgc catcggcgtg gtggcgctgg
      901 tggtgttaat tcgccacccg ttgatgccgg tgtatatcga tattttgcgt aaagtggttg
      961 gggtttaggg gatagccttg tgcgggttgc ctgatgcgac gcttgccgcg tcttatcagg
     1021 cctgcaaaac gcactacccg taggtcggat aaggcgttca cgccgcatcc gacagtgcat
     1081 attaaccttt aattcccaac agcgccgaag cactcgcctt accgctcaac aactcatcgg
     1141 tcttaccctg ccaggcgata cgcccgtcgg cgaccaccac cgagcgcgtg gcgatccgcg
     1201 ccgcatcttc cacactgtgc gacaccatca agagcgtcat tttttgctgt tggcagctcg
     1261 tgctcaccag cgtcagcatc tcctgacgta gcgccggatc gagcgcggaa aacggttcat
     1321 cgagcaataa aatgggctgt tcgcgtacca gacaacgcgc taacgccacg cgctgtcgtt
     1381 gaccgccgga aagctcgcca ggtaaccgcg ccattaaatt atcaatcccc atctggtggg
     1441 caatcgcgtg cattttcttc tgctgcgccg catttagttt caaccccgga tttagcccca
     1501 gcccgatgtt ctgcgcgacc gtcaggtggc tgaacaggtt gttctcctga aacagcatcg
     1561 acaccggacg gcgtgacggc ggcgtcgtgg tgtgatcaac gccgtcgata gtcagcaaac
     1621 cgctggctgg cgtcagaaaa ccggcgatca aattaagcag ggtactttta cctgcgccgc
     1681 ttggcccgag gatcgccacc tgctcgccgc gttccaccgt taagctaaaa cgcatcggca
     1741 aatggtggta aagccaggtg atatcagtca gttttaacat ttcgccccgg tatattttca
     1801 atcacggtaa acagcagaaa acagagcagc agcagaatta acgcggtgac cgcgccgtcc
     1861 tggctgcgat aggagccaat ttgctggtag agataaaacg gcagggtacg gaaatcatcg
     1921 ttaccaaaca atgccaccac gccaaaatca ccaatcgaca gcacacaagc aaaggccagc
     1981 gcctgcgcca gtggacgttt cagggcgcgc agctccacca cttttaagcg tgaccagcct
     2041 tcaatgccca gcgactgaca caacatgctg tagcgggcgg tgatatcgcg catcgggttt
     2101 tccagtactt tcagcgcata agggattgcc attaacgcat tggtgaaaat cacaatgccg
     2161 tcagcagatt gtggcaggcc gatagtgttg ttgagcagta aaaagaagcc cgtagccagc
     2221 acaatccccg gcatggcgag gatcaacatg ccgctcatct ccagcgcctg acccgccagc
     2281 attttctgcc gcgcccgaag ttcgcgactg ctccatagca gcatcatggt cagcactacg
     2341 cacaatacgc ctgccgccag cgcaatacgc aacgaggtcc acagcgcctg ccacagcacc
     2401 ggttgtgcca gcacttccgg caactggcga tttaccccat cgacgatcac cgccagtaac
     2461 ggtggcagca acagcagcag cgccagcaca attaacgccg tgtcgcaaat gcgactatgc
     2521 agacgatcgt ccgggtcgcg ccagccttgc agcagtgtgg taccaggcgc aatggcctta
     2581 ctcaatcgct gactcaacag caccagcccg aggcagcaca ccatctggat cagcgccagc
     2641 attgccgcgc gggcaggatc gtagtcgtaa ctcagtgcct gatagattgc cagctcgata
     2701 gtggtcgcct gcggaccgcc gcccagcgat agcacggtgg cgaagctggc gaaacagagc
     2761 ataaagatta gtgcggcaac cggcgggatt tgtcgccgta accacggcca ttcgacgaaa
     2821 cggaaaaaat gccagccgcg catcccaagt tgggcggcaa gctgacgttg ttcgccggga
     2881 atgttttcca gtgcctggag taataaacgg ctagccatcg gcagattaaa aaacacatgg
     2941 gccagcaaaa tgccttgcag gccgtagggc gaaaaggtcc actccagacc aagtgattgc
     3001 cagagcgagg ccagccagcc ctggcgacca tagacgctaa gaatgccaaa aacagcaacc
     3061 agcaccggga ggatcaaggt cattgcgcac agacgcaaca gcatctgtcg cccaggaaag
     3121 cgcctgcgat agagggcgcg ggcgaggaat attgcgggta cgacagagag cagcgccgag
     3181 agaaacgcct gccagaagga gaagcgcacc acatgccaca ggtagctgtc ctgccagact
     3241 gccacccagt catcctgcgg cgcgttccac cacagggcga gaaacgccgc cagtgcaacc
     3301 gccaccacca gcgtggcggc gcttacgcct ggaattaacc agccgggaat taacggctga
     3361 cggcgcgttg ccattcgcta atccatgcct gacgttgtgc cgccacttcg gcgggtgtga
     3421 actctagcgt ggtggctggt ttggtcaatt gttcaaatcc tgcaggcaga gtgacgtttg
     3481 ctaccggata catccagttg ccggttggga tcgcattctg aaaagccgga gaaaccataa
     3541 actggaggaa tttttgcgcc agctctggct gcttgctggc agcggtgcgg gcggcgactt
     3601 ccacttgcaa atagtgacct tcgctgaagt tcgcggtggc gtagttatct ttcttctctt
     3661 cgagaatgtg ataagccgga gaggtggtgt aactcaggac cagatcgctt tcacctttta
     3721 aaaacaggcc gtaggcttcg ctccagcctt tggtgaccgt gaccgttttc ttcgccagtt
     3781 tctgccaggc ttgcggggcg ttatcgccat agactttttg catccatagc aacagaccca
     3841 gccccggtgt actggtacgt ggatcttcat aaatcacccg ccagttttga acgctctcaa
     3901 ccaattcttt caggctttgc ggcggatttt tcagtttgtt cttgtcatag acgaaggcga
     3961 aatagccgta atcaaacggt acgaaagtgt cattattcca gccgccagga acgttaacgg
     4021 cttccgctgc cacaccgctt ttagcaaaca gcccggtttt gctggcggcg tctaacagat
     4081 tgttatccag ccccagcacc acatcggctt tgctgttttt gccttccatc cgtagacggt
     4141 tgagcagcga aacgccatct tccagcgcca ccagtttcag ttcgcaatta cagtctgctt
     4201 caaaggcttt tttgactacc ggaccaggtc cccagtcagc ggcaaaggaa tcgtaggtat
     4261 aaacagtcag aacaggttta gcgaaaacag gcgctgtgca cagcaacagc aggggaagac
     4321 atttttttaa cactttgcac ctcacaaaag agtggcaaag gacttgagaa ggagcctcaa
     4381 atcccttcgc cggcgttatc cggatcaggt tcgacgggta ttttctcagc gcacgcggct
     4441 gcgtggcacc ccgttgagaa cggcgttagt gtagtgattt tgttatcaac cagcaatcat
     4501 ggatccggtg gtgcaaacca cgctgattta aaatcgaacc agccgagggt gttcatgcgc
     4561 aggccgcgca tactacgttg cccctgaatg atcagccagt ggtggataag cggcaccatt
     4621 gctttgctgg cgaccagttg ctggcaccag ttcgccaggt tcatttcgcc attgcgccag
     4681 cgggcggcgt cggcttgcca gtcgatcgga atacagtgtt ggagcagcgg cacctcgcac
     4741 aagtgcgcga acagcgaaaa atccagcggc agcgtaaagt tggcgctgtt aagccagata
     4801 tcgctctcga tctctccttc atgccactga tcgtagctga tctctttgat tttcatcgtg
     4861 acctggtgac ttgccagaat ctgctgcatg atcccggcaa tcacccgatg ctcgctgtga
     4921 tcctgataaa aggtcagggt gaggctttcc agaccagccg gtttttcgct ctttatggtg
     4981 cgggcatggt gccaacgggg gagcagtcca taagccggga accacagttg ctggtattgt
     5041 tcctcggcga aatagaccag attcgtcgga gaaagcacat agcttaccca gtctctgact
     5101 tgctgattcg cccctcgatg ggtgcggctg tcgaacagta aatagtagca accttcctcc
     5161 aggcggcttt caatctcttt ttcctcgccc tgtggacctt ttagcatcag ccctccggct
     5221 ggctcgtcgg caatttccgg cagaacccag acgttaactt cgtcgattaa tgcccggtaa
     5281 ccgaagaagt catcgaatgc ctgaattttc agttgattgg tgctgttgcg gatcaccgca
     5341 tacggaccgg tgccgatggg atggctggca aagttactga gggtttccca ttcgcgcggc
     5401 aggatcattg ccggaacttg ccccagcagt aacggcaacc agcggtccgg ttgcgtgaga
     5461 tggatatcca gtgtccaggg cgtcggcgac acaatgtcag caatatgcga atagagcgga
     5521 agcgtattga ttcgttttaa agaggcgatc acatcgtcca tttccagttc acgaccatgg
     5581 tgaaaatgga ctcctggacg caaaaagaaa cgccagtgaa gcggtgatat ttgctgccag
     5641 tggtgggcga tgtctgcttc cagttcccca ttttcctcat ttatgcgcgt tagcgaactg
     5701 aagatttgcc gggcgatatg ggtttcagaa cggcgcaatg cgctgccagg tagcagatta
     5761 cgcaacggac gatagtagag cacgcgcagg atgtgccgcc cctggcggaa gctgcggccc
     5821 agatgagaaa ccagcatttg ccgcacagtc gctttgtcgc caaccaactg cactagttgg
     5881 tcgatacgat cctgctccag caggtcttcc gcccgctgtt gctgaagcgc cagcccggta
     5941 tagaggaatg tcagacgcga gcgtttaccg cgcccgactt ccgcttccca cgtcagccag
     6001 ccgcgatcct gcatggtgct gagcagggtg cgcatatgac gacgcgagca actcagtaac
     6061 gctgccagtt cgttgagcgt ggtctcctga gatttaccct cgcagcattg ccacaggcgg
     6121 atgaactgtt gttgcagacg agcagatggc ataaaagggg aactcctgcg caaaagacag
     6181 caattttatt ttccctatat taagtcaata attcctaacg atgaagcaag gaggtgcccc
     6241 atgcgtcagt tttatcagca ctattttacc gcgacagcga agttgtgctg gttgcgttgg
     6301 ttaagcgtcc cacaacgatt aaccatgctt gaagaactga tgcaatggga ggggagtcat
     6361 tctgactact gatttgcgga catcatgtgt gactgagtat tggtgttaat cgccacgcca
     6421 gcagtgatta tctgctggct tttttctttt tggcgattga gtgccggacg gtgtacaaac
     6481 taacgcataa acgccggttg cttctcttca taagacgcaa ttgcgtcgtc gtgctgcaga
     6541 gtcaggccga tgctgtccag accgttcatc atgcagtggc ggcggaaggc atcgatggta
     6601 aagcgataag ttttctctcc cgctttcacc tcttgcgctt ccaggtctac atcaaaatga
     6661 atccccggat tggctttcac cagcgcaaac agctcatcca cttctgcatc gcttaatttc
     6721 accggcagca gctggttgtt aaagctattg ccgtagaaga tgtcagcaaa actcggcgca
     6781 atcaccacct taaaaccgta atcggtcaat gcccacggcg cgtgctcacg cgaggaaccg
     6841 cagccgaagt tttctcgtgc cagcaaaatg gaagcgccct gatactgcgg gaagttcagc
     6901 acgaagtccg ggtttggctg ttggcctttt tcatccagaa aacgccagtc gttaaacaga
     6961 tgcgcgccaa aacccgtacg ggtcactttc tgcaaaaact gtttcgggat gattgcatcg
     7021 gtatcgacat tggcggcatc caacggaacc accaggcctg tgtgtttgat aaatttctct
     7081 gccatggtgt gctccttatt taatgttgcg aatgtcggca aaatgaccgg tcacagccgc
     7141 ggcggcagcc attgccgggc tgaccagatg cgtgcgcccg ccgcgtccct ggcggccttc
     7201 aaagttacgg ttgctggtgg aggcgcaacg ttcgcccgga ttcaggcggt cgttgttcat
     7261 cgccagacac attgaacaac ctggcaagcg ccattcaaaa ccagcttcga taaaaatttt
     7321 gtccagacct tccgcttccg cctgggcttt aaccggaccg gaaccgggaa ccaccagcgc
     7381 ttgtacgcct ggcgcgactt tccggccttt ggcgatttcc gctgctgcac gtaaatcttc
     7441 aatacgcgag ttagtacagg aaccaataaa cactttgtcg atagccactt ctgtcagcgg
     7501 aatacccggt ttcagcccca tataggccag cgctttttct gccgacgcgc gttcaaccgg
     7561 atcggcaaac gaagccggat cgggaatatt gtcgttcaca gaaatcacct ggcccggatt
     7621 ggtgccccag gtgacctgcg gcgaaatctc ttctgcttgc agagtgacaa cggtatcgaa
     7681 agttgctcct tcgtcggttt gcagggtttt ccagtaggca acggcgtcgt cgaaatcttt
     7741 gcctttcggc gcatgcagac ggcctttaac atagttaaag gtggtttcgt ccggtgcaac
     7801 cagaccggct tttgcgccca tttcgattgc catattgcac agggtcatac gaccttccat
     7861 gcttaaatca cggattgctt cgccgcaaaa ttccaccaca tgcccggtac cgcctgcgct
     7921 gccggtttta ccaataattg ccagcacgat atcttttgcg gtaatgcccg gcgcggcttt
     7981 gccctggact tcaattttca tggtcttcgc ccggccctgt ttcagggttt gcgttgccag
     8041 tacgtgttca acttcggaag tgccgatacc aaaggccagt gcgccaaacg cgccgtgggt
     8101 ggcggtatgt gagtcgccgc agacaatggt catccccggc aaggtcacgc cctgttccgg
     8161 ccccattacg tggacgatcc cctgatacgg gtgattcagg tcatacagct cgacgccaaa
     8221 ttctttgcag tttttgatca gctcctgcat ctggatgcgc gccatttcac cgcaggcatt
     8281 aatgtctttc gtctgggtag agacgttgtg atccatggtg gcgaaggttt tgcccggctg
     8341 acgtaccgag cgaccgtggg cgcgcagacc atcgaacgcc tgcggtgagg tcacttcatg
     8401 caccaggtgg cggtcgatat ataacagcgg ggtttcgttt tcggcttcgt acactacgtg
     8461 agcgtcgaat aatttttcgt ataacgtctt agccatgatt acaccccttc tgccacatag
     8521 cgggcaatga tatcgcccat ttcatcggta ctaacggcgg cagcgccacg ggctaaatcc
     8581 ccggtgcgaa tgccttcttc taatgcgcgg ttaatggcgc gttcaatggc ggaagccgca
     8641 tcatcggcat ccaggctgta acgcagcagc agcgccagcg acagaatttg tgcaatcggg
     8701 ttggcgatat ttttacctgc gatatcgggt gccgagccgc ccgccggttc atacagacca
     8761 aaaccttgct cgttcaggct ggcggaaggc aacatcccca ttgagccagt gatcattgcg
     8821 cactcatcag acagaatgtc gccaaacagg ttggagcaca gcaagacgtc aaactgtgat
     8881 ggatctttaa tcagctgcat ggttgcgttg tcgatgtaca tatgcgccag ttcgacgtcc
     8941 gggtattccg tggcgatctc gttaacgatc tcccgccata aaatagagga ttgcagcacg
     9001 ttggctttgt cgattgaggt gactttgtgg cgacgcttgc gggcagattc aaatgcgatg
     9061 cgggcaatac gctcgatctc aaaacggtgg tacacctcgg tatcaaacgc tttttcatat
     9121 tgtccgctac cttcgcggcc ttttggctga ccgaaataga tgccgccggt cagttcgcgc
     9181 acacacagga tgtcaaagcc gtttgcggca atgtcagcac gcagcggaca gaatgcttcc
     9241 agcccctgat acagttttgc cggacgcaga ttgctgaata atttgaagtg cttacgcaaa
     9301 ggcagcagtg cgccgcgttc tggttgctgg tctggcggca aatgttccca cttcggacca
     9361 cctaccgagc caaacaacac ggcatcggct tgctcgcaac cttcaactgt cgcaggcggc
     9421 agtggctgcc cgtggttatc aatggcggcg ccgcctacat cgtaatggct ggtggtgata
     9481 cgcatcgcaa atcggttgcg cacggcatcc agcactttca gcgcctgggt catcacttcc
     9541 ggaccaatac cgtcccccgg caatacggca atatggtaat tcttcgacat cacacggttt
     9601 ccttgttgtt ttcgttgtgt tgagctttgc gttgcaactc tttttcgact tctgcggcac
     9661 gccagatatt gttcagtacg tgcaccatgg ctttggcgga ggactcgaca atatcggtgg
     9721 ccaggccgac gccgtggaag cggcgaccgt tgtagttggc gacaatatcc acctgaccca
     9781 gcgcatcttt accgtggcct ttggcggtca ggctgtattt caccagttcg acgttatagt
     9841 cagtgatgcg gtttatcgcc tggtagacgg catcgaccgg accgttaccg ttggcggctt
     9901 ctgctttgac ttcttcgcca caggccagtt tgacggcggc agtggcaata tcgttagaac
     9961 ctgactgcac gctgaagtaa tccagacgga aatgctccgg ctcttcttgc tgcttaccga
    10021 tgaaggccag cgcctccaga tcgtaatcaa acacctggcc ttttttgtcc gccagcttca
    10081 ggaaagcgtc gtacaggttg tctaaattat attcactttc tttatatccc atctcatcca
    10141 tgcgatgttt caccgccgca cgccccgaac gagaggtcag attcagctgg atttggttca
    10201 gaccaataga ttctggtgtc atgatttcgt agttttcgcg gtttttcagt acaccatcct
    10261 ggtggatacc ggaggagtgt gcgaatgcgc cgctgccaac aatggctttg tttgccggga
    10321 tcggcatatt acaaatctgg ctaactaact ggctggtgcg ccatatctcc tggtgattga
    10381 tggcggtgtg gacgttgaga atatccttac gaactttgat cgccatgatg acttcttcaa
    10441 gggaacagtt accggcacgc tcgccgatcc cgttcattgc gccttctacc tgccgtgctc
    10501 cggcatgtac cgccgccagt gagttgccga ccgccaggcc caaatcgtcg tgggtatgta
    10561 cggaaataat ggctttgtcg atgttgggta cgcgttcata caggccgctg atgattccgg
    10621 cgaactcaaa tggcatggtg tagcccacgg tgtccggaat gttgatggtg gtggcaccgg
    10681 cattaatcgc cgcttcgacc actcgcgcca gatcggcaat tggggtgcgt ccggcatctt
    10741 cgcaagaaaa ttcaacatca tcggtgtaat tacgggcgcg tttcaccata tagatagcgc
    10801 gttcgatcac ttcgtccagc gtgctgcgca gcttggtggc gatgtgcatt ggcgaagtgg
    10861 caataaaagt atggatacgg aaggcttcgg cgactttcag ggattcggct gccacgtcaa
    10921 tatctttttc cacgcagcga gctaacgcgc atacacggct gtttttaacc tggcgagcga
    10981 tggtctgcac tgattcaaaa tcacccggcg aagagacggg gaaaccgact tccatcacgt
    11041 caacacccat acgctcaagg gccagcgcaa tttgcagttt ttctttcaca ctcaagcttg
    11101 cctgtaacgc ctgttcaccg tcgcgcaatg tggtatcgaa aataatgact tgctggctca
    11161 tggtttgggt ccttgtctct ttttgagcgc ctcgcttcgg gcataaaaaa acccgcgcaa
    11221 tggcgcgggt ttttgtttga ctgcgtgctg gcttaatgct ggatgccgct cactcgtcta
    11281 ccgcgcaaag aagatgcgtt tagtagtagt agaccgataa agcgaacgat gtgagtcatt
    11341 aaatcagctc cagatgaatg cgatatgctt ttagagttac tggatacaaa aacggatgtc
    11401 aaccctgaag caataaaaac gtcacgccat tgtgagttcg gcatccgtaa aattagctaa
    11461 ttgtgctgcg gtggttaaaa caagcgatat taatttctgc ttaaccgccg atgcttttca
    11521 tcggttgaca tatttcagaa taattttttg catctaatca acgagggaaa gggggataaa
    11581 atgcacgcgt tacaaaacct atcctgatga tttgtattga attat
//
LOCUS       DAZFRG010000152        11597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFRG010000152 DAZFRG010000000
VERSION     DAZFRG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.152"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            148..1536
                     /gene="narU"
                     /locus_tag="ACHKUC_003289"
     CDS             148..1536
                     /gene="narU"
                     /locus_tag="ACHKUC_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO6619497.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1631..5371
                     /gene="narZ"
                     /locus_tag="ACHKUC_003290"
     CDS             1631..5371
                     /gene="narZ"
                     /locus_tag="ACHKUC_003290"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO6619498.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMISYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDTVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADNAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCIGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5368..6912
                     /gene="narH"
                     /locus_tag="ACHKUC_003291"
     CDS             5368..6912
                     /gene="narH"
                     /locus_tag="ACHKUC_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6619499.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6912..7607
                     /gene="narW"
                     /locus_tag="ACHKUC_003292"
     CDS             6912..7607
                     /gene="narW"
                     /locus_tag="ACHKUC_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6619500.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPILTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7604..8284
                     /gene="narI"
                     /locus_tag="ACHKUC_003293"
     CDS             7604..8284
                     /gene="narI"
                     /locus_tag="ACHKUC_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6619501.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRVTSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8364..9257
                     /locus_tag="ACHKUC_003294"
     CDS             8364..9257
                     /locus_tag="ACHKUC_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO6619502.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDNILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(9353..10198)
                     /gene="nhoA"
                     /locus_tag="ACHKUC_003295"
     CDS             complement(9353..10198)
                     /gene="nhoA"
                     /locus_tag="ACHKUC_003295"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO6619503.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10373..10942
                     /locus_tag="ACHKUC_003296"
     CDS             10373..10942
                     /locus_tag="ACHKUC_003296"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO6619504.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11259..11474)
                     /locus_tag="ACHKUC_003297"
     CDS             complement(11259..11474)
                     /locus_tag="ACHKUC_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6619505.1"
                     /translation="MFIRPVKPSDVEPLMDMLLDRDQFDQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCASEMMTNDV"
BASE COUNT         2753 a         2992 c         3009 g         2843 t
ORIGIN      
        1 atattctgcc cccgcagtta cgctttctta tctattcttc taatcagcac ttcacctgat
       61 tcatccttaa ttctaataac tggctattgc gccttacctc ctgttttgtc tttttttcat
      121 taccaatgtg tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat
      181 cttttacgcg actggaaacc agaaaatccg gccttctggg aaaataaagg aaagcatatt
      241 gcacgaagaa acctctggat atcagtcagt tgcctacttc ttgccttctg tgtctggatg
      301 ctatttagcg cagttactgt taatcttaat aaaatcggtt ttaatttcac taccgatcaa
      361 ctctttttat taaccgcatt accctccgtt tctggcgcat tattgcgtgt cccctactcc
      421 tttatggtgc ctatattcgg tggacgccga tggacggttt ttagtactgc aatcctgatt
      481 attccttgcg tctggctcgg aattgccgtg caaaatccga ataccccttt tgggatattt
      541 atcgttatcg ctttgctatg cggttttgca ggtgcgaact ttgcttcgag catgggcaat
      601 atcagtttct tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta
      661 ggaaacttag gtgtaagtgt gatgcagctg gttgcaccgc tggtcatttt tgtacccgta
      721 tttgcctttc tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg
      781 aatgccgcat ggatttgggt gccattactg gcgattgcca cgatcgccgc ctggtcaggg
      841 atgaatgata tcgccagttc acgcgcctca attgccgacc agctccctgt cttacaacgc
      901 ctgcatctct ggctgctgag cctgctttat cttgccacct tcggttcgtt tatcggtttt
      961 tctgcgggtt ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg
     1021 gcgttctttg gcccatttat cggtgccatc gcgcgatcgg ttggtggtgc tatttccgat
     1081 aaattcggcg gcgtgcgggt gacgttgatc aacttcattt ttatggcgat tttcagcgcc
     1141 ctgctgttcc ttaccttacc gggcacaggc tccggtaatt ttatcgcatt ttacgccgta
     1201 tttatggggc tgtttctgac cgcgggtctg ggaagtggtt ctactttcca gatgatcgcc
     1261 gtcatctttc gccagataac aatttatcgg gtgaagatga aaggcggtag tgatgagcaa
     1321 gctcaaagag aagccgtcac cgaaacggca gccgctctgg gctttatctc agccattggc
     1381 gcagtgggcg gcttttttat tccgcaggcg tttggcatgt cgctcaatat gaccggctct
     1441 ccggtgggcg cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct actgacctgg
     1501 ctggtttatg gtcggcggaa gttcagccaa aaataagaga tcgatgcctg acacgtctta
     1561 tcaggctaca accgtgtcgc atccgacaag gcatcagatc ccaattcatt cctggagcag
     1621 gagttatgtc atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac
     1681 ctttgccgat ggtcacggac aggtgatgca tagcaaccgc gactgggagg acagctatcg
     1741 ccagcgttgg cagttcgaca aaatcgtgcg ttccacccac ggtgttaact gtacaggctc
     1801 ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga
     1861 ctacccgcgc actcgccctg acctgccaaa tcatgaacct cgcggctgcc cgcgtggcgc
     1921 aagttactcc tggtatctct acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg
     1981 actgattgaa ctgtggcgcg aagccctcaa gcaacacagc gatccggtac tggcgtgggc
     2041 atcgattatg aacgatccgc aaaagtgcct gagctacaaa caagtgcgtg gacgcggtgg
     2101 ctttatccgc tccaactggc aggagctaaa tcagctgatt gccgctgcta acgtctggac
     2161 catcaaaact tacggcccgg atcgcgttgc cggattctca ccgattccgg cgatgtcgat
     2221 gatttcttac gccgccggaa cacgctatct gtcgctgtta ggtggcacct gtttaagctt
     2281 ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg acctggggcg agcaaaccga
     2341 cgtaccggaa tctgccgact ggtataactc cagttacatc atcgcctggg ggtctaacgt
     2401 accgcagaca cgtacgccgg acgcccattt ctttaccgaa gtacgctaca aaggcactaa
     2461 aaccatcgcc attacccctg actactctga agtggccaaa ttgtgcgacc agtggctggc
     2521 accgaaacaa ggcactgata gtgccctggc aatggcaatg ggccatgtca ttttaaaaga
     2581 gtttcatctc gataatccca gcgattattt tatcaactac tgccgccgct acagcgacat
     2641 gccgatgctg gtaatgctgg aacctcgcga cgatggcagc tacgttcccg ggcgcatggt
     2701 ccgcgcatct gacctggtgg atggactggg cgaaagcaat aatccgcagt ggaaaaccgt
     2761 ggcagttaat accgcaggtg aactggtggt gccgaacggt tcgattggtt ttcgctgggg
     2821 agaaaaaggc aaatggaacc tggaatccat tgccgccggt acggaaaccg aattgtcgtt
     2881 aaccctgctc ggtcaacacg acaccgtcgc aggcgtggcc ttcccctact ttggcggcat
     2941 tgaaaatcca cattttcgca gcgtaaaaca caatcctgtc ttggtgcgcc aattgcccgt
     3001 taaaaacctg acattagccg atggtagcac ctgtccggtg gtcagcgttt atgatttggt
     3061 actggcgaat tacggcctcg atcgcgggct ggaagatgaa aacagcgcga aagattacgc
     3121 tgaaatcaaa ccttataccc ctgcctgggg cgagcaaatt accggcgttc cgcgccagta
     3181 tattgaaacc atcgcccgtg aatttgccga taccgcccat aaaacgcatg ggcgctcgat
     3241 gattattctc ggcgcaggtg ttaaccactg gtatcacatg gacatgaact accgcgggat
     3301 gatcaatatg ctgatcttct gcggttgtgt cgggcaaagc ggtggcggct gggcacacta
     3361 tgtcggtcag gaaaaactgc gcccgcaaac cggctggctg cctctggcct ttgcgctcga
     3421 ctggaaccga ccaccgcgcc agatgaacag cacctcgttt ttctacaatc actccagcca
     3481 gtggcgctat gaaaaagtca ccgcgcagga attgctctca ccgctcgccg atgccagtaa
     3541 atacagcggt cacctgattg atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc
     3601 tgcgccgcag ttggggcgta atccgctcgg gattaaagct gaagccgaca atgccggatt
     3661 atctcccaca gaatttaccg cccaggcgct gaaatcgggc gatttacgta tggcctgcga
     3721 acaaccagat agcggcagca atcatccgcg taatttgttt gtctggcgtt ctaacctgct
     3781 tggctcctcc ggcaaaggcc acgagtatat gcagaagtat ctgctgggaa ccgaaagtgg
     3841 gattcagggt caggaactcg gtgccagcga cgggatcaaa ccagaagaag tcgagtggca
     3901 aactgcggcg attgaaggca agctcgacct gctggtgacg ctcgacttcc gtatgtccag
     3961 tacctgcctg ttctccgata tcgttctgcc caccgccacc tggtacgaaa aagacgatat
     4021 gaatacctcg gatatgcatc cgtttattca tccgctttct gcggcggtcg atccggcctg
     4081 ggaatcacgc agcgactggg aaatctacaa aggtatcgct aaagcctttt cgcaagtgtg
     4141 catagggcat ctcggcaaag aaaccgacgt ggtattacaa ccactgctgc atgactcccc
     4201 ggcagagctt tcacagccgt gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat
     4261 tccaggcaaa accgcgccga atattgtggc ggtggagcgc gactaccctg ctacctatga
     4321 acgctttacc tcgctcgggc cattgatgga caaacttggc aacggcggta aagggatttc
     4381 gtggaatacc caggatgaaa ttgatttcct cggtaaactt aattacacca agcgtgatgg
     4441 cccggcacag gggcgtccgc tgattgatac cgcgattgac gcatcagaag tgattctggc
     4501 gctggcaccg gaaaccaacg gccatgttgc agtcaaagcg tggcaggcgc tgggcgagat
     4561 caccggacgc gaacataccc atctggcgct gcacaaagag gacgagaaga ttcgctttcg
     4621 cgatattcag gcgcagccgc gtaaaattat ctccagcccc acatggtctg gtctggaaag
     4681 cgatcacgtt tcctataacg cgggatacac caacgttcat gagttaattc cgtggcgcac
     4741 gctgtcggga cgccagcaac tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag
     4801 tctggtggct tatcgcccgc ctatcgacac ccgtagcgtc agtgaaatgc gccagatacc
     4861 gccaaacggc ttcccggaaa aagcacttaa cttcctgacg ccgcaccaga aatggggcat
     4921 tcactcaacc tacagtgaaa acctgctaat gctgacgctc tctcgcggtg gaccgattgt
     4981 ctggatcagc gaaaccgatg cccgagaact gaccattgtc gataacgact gggtggaagt
     5041 atttaacgcc aacggcgcgc tgactgcccg cgcggtggtc agccaacgtg taccgccggg
     5101 catgaccatg atgtatcacg cccaggaacg cattatgaat attcctggtt cggaagtaac
     5161 tggcatgcgc ggcggcattc ataactcggt cacccgcgtt tgtccgaaac caacgcatat
     5221 gattggcggt tacgcgcagc tggcctgggg ctttaactac tacggcactg tcggctctaa
     5281 ccgcgacgaa ttcatcatga tccgcaagat gaagaacgtt aactggctgg atgatgaagg
     5341 tcgcgatcag gttcaggagg cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt
     5401 aacctcgata aatgtatcgg ctgccatacc tgttcggtga cctgtaaaaa cgtctggacc
     5461 ggacgcgaag gcatggagta cgcatggttt aacaacgtcg aaaccaaacc gggcattggt
     5521 tacccgaaaa actgggaaga tcaggatgag tggcaaggcg gctgggtgcg tgatgtgaat
     5581 ggcaagatac gcccgcgtct gggcagcaaa atgggcgtga ttaccaaaat tttcgccaac
     5641 ccggtagtgc cgcagattga cgattactac gaacctttca ccttcgacta cgaacatttg
     5701 catagcgcgc cggaaggcaa acatattccc accgcccgtc cgcgttcact gattgacggc
     5761 aaacggatgg acaaagtgat ctggggccca aactgggaag aactgctggg cggcgaattt
     5821 gaaaaacgag cccgcgaccg caacttcgag gccatgcaaa aggagatgta cgggcagttt
     5881 gaaaacacct tcatgatgta cctgccgcgc ttgtgcgaac actgccttaa ccccagttgt
     5941 gtagcgacct gccccagcgg cgctatctac aaacgcgaag aagacggcat tgtgctgatc
     6001 gatcaggata aatgccgtgg ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc
     6061 tacttcaact ggaaaagcgg caagtcagaa aaatgtatct tctgttaccc acgaattgag
     6121 tcaggccaac ccaccgtgtg ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg
     6181 ctgctttacg acgccgaccg cattgaggaa gcggcaagca ccgagcgcga agttgacctc
     6241 tatgagcgcc agtgcgaagt gttcctcgat ccacacgatc cctcagtgat cgaggaagcc
     6301 ctgaaacaag gtattccaca aaacgtgatt gacgctgccc agcgttcgcc tgtctacaaa
     6361 atggcgatgg actggaaact ggcgctaccg ctgcaccctg aatatcgcac cctgccgatg
     6421 gtctggtacg ttcctccgct gtcaccgatt cagtcctacg ctgatgcggg aggtttgccg
     6481 aaaagcgaag gcgtgctgcc cgccatcgaa agcctgcgta ttccggtgca atatctcgcc
     6541 aatatgttga gtgccggtga taccggtccg gtactgcggg cgctgaaacg gatgatggcg
     6601 atgcgccact atatgcgttc acaaaccgtg gaaggcgtta ctgatactcg tgccatcgac
     6661 gaagtaggtc tgagcgtcgc ccaggtcgaa gagatgtatc gttacctcgc cattgccaac
     6721 tatgaagatc gtttcgtcat cccgacaagc catcgggaaa tggcgggcga tgccttcgca
     6781 gaacgcaacg gctgcggttt tacctttggc gacggttgcc acggttcgga cagcaaattc
     6841 aacctgttca acagtagccg tatcgatgcc atcaacatca ccgaagtgcg cgacaaagcg
     6901 gagggcgaat aatgcagatc ctcaaagtga tcggcttgtt gatggagtat ccggacgagc
     6961 tgttatggga atgcaaggag gacgcgctgg cgttgatccg ccgcgacgca ccgattctta
     7021 cggattttac ccgcaacctg cttaacgcgc cgctgctgga taaacaggcc gaatggtgcg
     7081 aagtgtttga ccgtggacgt accacatcgc tgctgctgtt cgaacatgtt catgccgagt
     7141 cccgcgatcg cggccaggca atggtggacc tgctggcgga gtatgaaaag gtcggtctgc
     7201 aactgaattg tagggaactg cccgattatc taccgctgta tctggagtat ttaagcgtac
     7261 ttccggacga tcaggcgaaa gaaggattac tcaacgttgc gccgatcctc gccctgcttg
     7321 gcggacgctt aaaacaacgc gaggcaccgt ggtacgcgtt atttgacgcc ctgttgcaac
     7381 tggcaggaag ccctctgtca agtgacagtg tcacaaaaca gattcgcagt gaagagcgtg
     7441 acgacacccg ccaggcgctg gatgcggtgt gggaagaaga acaggtgaag tttattgaag
     7501 ataacgccac ggcgtgtgac agctcgccgc ttaatcaata tcagcgacgc tttagccagg
     7561 atgtcgcgcc gcagtatgtc gacatcagtg cgggaggtgg gaaatgattc agtatctgaa
     7621 cgtctttttt tacgatatct acccgtactt gtgtggcacg gtttttatcc ttggaagctg
     7681 gctgcgctac gattacgggc agtatacctg gcgtgccgcc tccagccaga tgctggataa
     7741 acgcgggatg gtgctatggt cgaacttgtt ccatatcggc attctgggga tcttcttcgg
     7801 tcatctgttc ggcatgttaa cgccgcactg gatgtacgcg tggtttctgc cggtggcggt
     7861 aaaacagcag atggcgatga tcctcggcgg aatatgcggg gtattgacgc tgattggcgg
     7921 tgcggggcta ctggtgcgtc gtttaacaaa tccgcgtatc agggtgacgt cttctggtgc
     7981 ggatatcctg atcctcgcca ttttgttaat tcagtgcatt cttgggttaa ccaccattcc
     8041 tttttcagca caacatccgg acggcagtga aatgatgaag ctggtgggct gggcgcaaag
     8101 cattgtcact ttccagggcg gtgcatctac gcatcttgat ggtgtcgcct ttatatttcg
     8161 cgttcatctg gtgcttggaa tgaccatctt cctgctcttc ccgttcaccc gactggtgca
     8221 tgtctggagc gcgccgtttg agtactttac tcgtcggtat cagattgtgc gttcgcggcg
     8281 ttaattctca cgcgggccgg atagcccttc gctctccggc cacttgcagg ctatgatagt
     8341 tctccagcca caggagaaca ttaatgaaac cgcaggttta ccacgtcgat gcttttacct
     8401 ctcaaccatt tcgcggcaat tctgccggtg ttgttttccc cgccgataat ctcagtgaag
     8461 cgcaaatgca gcttatcgcc cgcgagttag gtcattcgga aactgctttt ctgctgcaca
     8521 gcgacgacag cgatgtgcgc atccgctact ttacgccaac ggttgaagtt ccgatttgtg
     8581 gtcacgcgac agtagctgcg cactatgtac gtgcaaaggt gttaggttta ggaaattgca
     8641 cggtctggca aacatcgctg gcaggaaaac atcgtgtgac tatagaaaag caaaatgatg
     8701 attatcgtat ttcgctggaa caaggtactc caggctttga gccaccgctg gaaggtgaaa
     8761 cacgtgcagc gattatcaac gcgcttcatc tcactgagga caatattctg ccaggcttgc
     8821 caattcaggt ggcaacaaca ggacactcaa aagtgatgat cccactgaag ccggaagtgg
     8881 atatcgacgc cctttcgcct gatcttgctg cgctgaccgc tatcagcaaa cagattggct
     8941 gcaatggttt cttcccgttc cagatccgcc caggcaaaag cgaaaccgac ggtcgcatgt
     9001 tctcgcctgc gattggtatt gtggaagatc cggtgactgg caatgccaat ggcccgatgg
     9061 gtgcatggtt agtacatcac aacgtattgc cccacgatgg caacgtgttg cgtgttaaag
     9121 gccatcaggg gcgcgcatta gggcgtgatg gtgtaattga tgtgacggtg acaattcgag
     9181 ataaccaacc ggagaaagtt accatttccg gcacggcggt gattctattc catgctgaat
     9241 gggccattga tttttgatgc cgttgcctga tgcgcttcgc ttatcaggcc tacaaccgga
     9301 ctaatgtagg ccggataagg cgtttacgcc acatccggca acctgacata aattattttc
     9361 ccgcctccgg gtgcgtatca aacgccgcca tcaccagcgc taactcatcc acagtaaagc
     9421 catgtttcgc atcatctacg cccagaccaa actgttcctg catcactgca tataacgacg
     9481 ccacatctgg tagatttcgc tgctcaactg cgtgcccatt ttcataatgg gtaaaatgaa
     9541 aattggtcag cgttagcttg ccaccgtccg gcaaatggcg acacatcagc aaatgatggc
     9601 gaaaatgcga ctgcggccag tgcgccgacc agaaattgcc catcacataa tcgctttgtt
     9661 gctgctcgca gagatcaaaa cggtacatcg actgccagtg ctgatgatga ttaaactgca
     9721 atacccaatc atcaccctct tgcaacaacc gatactctcc gtgtggtgtg gtttgcatga
     9781 tatccggcat taaacgaatc ggcgcggtta gcgtctgccc accgaaaccg acatcagcaa
     9841 tccatttttc cccttccagt tccaccaaca gcaaacgatg ggtgcgcggc ggtaatgctg
     9901 gcggatttga taacactacg cgacctaaca agctgcgaac gttaaacccc agctcgcgta
     9961 acacccgctc aaacacgcca ttctgctcaa aacagtaacc gccacgacgg gctatcacca
    10021 gtttatcttc cagcgattga tcatcaagct gtatttccct cggcagcaaa acgtcgagat
    10081 tttcaaacgg aatggtgcaa ttgtgtttca ggtgcaatgc acgcagcgta tcaatattgg
    10141 ccgcagcagc tcccaaccag ttaatacggg caaaataatg attcagaatg ggcgtcatca
    10201 ggcgtttccc ttagtggttt tctcaactta taacctggaa tagacgccgc actggctatt
    10261 ttgtgcgatc gcggacatgc atcacgttcg caaatcgcca atcccgttaa tttcgttact
    10321 tattgcgtta cgagctacgc ttttaaaaca cccttcatag cgaggcgata tcgtgagcca
    10381 attcattccc gtcgaattac accatgccag ccgtctgtta aatcatggcc caactgtcat
    10441 gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt atggcagcag cctggtcgat
    10501 gcccgtagag tttgaaccgc cacgcgtggc gattgtggta gataaatcga catggaccag
    10561 ggagttgatt gaacgtaacg gtaagtttgg catcgttatc ccgggcgttg cggcgactaa
    10621 ctggacgtgg gcggtgggaa gtgtgtcggg tcgtgaagaa gataaattta attgctacgg
    10681 cattccggta gtgagaggcc cggtgcttgg tttgccgctg gtcgaggaaa aatgtctggc
    10741 gtggatggag tgtcgcctgc tacctgcaac ttccgcgcaa gaaaaatacg atacgctatt
    10801 tggcgaagtg gtatcagcag cggcagacgc acgggtattt gtcgaaggcc gctggcagtt
    10861 tgatgatgat aagctcaata cgttgcatca tttaggtgct ggcacgtttg ttaccagtgg
    10921 taagcgagtt accgcgggct aaagaatcat atgcagcaac caagttgttt aagcaatgtt
    10981 tccatatgag ttaaagcata ttttatctca cgattgcata tttttagtga agattaattt
    11041 atcctcacca acatcatagt aattgcgtat tatcgcctca ttgataaagc cctgttttgt
    11101 ataaaatgcg cgggcggcac taaaatcgat atggctggcg gtctctacta atagtagtct
    11161 tgcctgttta attgtgagct cttttctatt tcgttaatca gcagatgtct cactccctgg
    11221 cgctgatggg aaggactgat ccagagcatt aataaatttc agacatcatt tgtcatcatt
    11281 tcagaagcac agtaggcaat ccctgccagt cccaagtgtt ctgcactaaa ccataaatcg
    11341 gcagattgac ctgagaaata atgtgttagt gttttttgta catgatgaag gccatcttga
    11401 tcaaattgat ccctgtcaag cagcatatcc attaatggtt caacatctga aggtttgact
    11461 ggtcgtataa acatggaact ccttatttat tgatgaatat ttgaaaattg tttgcatgga
    11521 acgcggttaa cctgaatttt cagcggaagt ttcactcaaa ggctttatta atccgctcct
    11581 tatgtccttt tacagca
//
LOCUS       DAZFRG010000153        11511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFRG010000153 DAZFRG010000000
VERSION     DAZFRG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.153"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..2440
                     /gene="ynfE"
                     /locus_tag="ACHKUC_003298"
     CDS             14..2440
                     /gene="ynfE"
                     /locus_tag="ACHKUC_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO6619506.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAEAAV
                     QQANEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVKQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAHKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2501..4924
                     /gene="ynfF"
                     /locus_tag="ACHKUC_003299"
     CDS             2501..4924
                     /gene="ynfF"
                     /locus_tag="ACHKUC_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO6619507.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWILSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQTNPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNTNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4935..5552
                     /gene="ynfG"
                     /locus_tag="ACHKUC_003300"
     CDS             4935..5552
                     /gene="ynfG"
                     /locus_tag="ACHKUC_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO6619508.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNGNSRPTGDTTGYLANPEEV"
     gene            5554..6407
                     /gene="ynfH"
                     /locus_tag="ACHKUC_003301"
                     /pseudo
     CDS             5554..6407
                     /gene="ynfH"
                     /locus_tag="ACHKUC_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
     gene            6450..7064
                     /gene="dmsD"
                     /locus_tag="ACHKUC_003302"
     CDS             6450..7064
                     /gene="dmsD"
                     /locus_tag="ACHKUC_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO6619509.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDSWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7223..8515
                     /gene="clcB"
                     /locus_tag="ACHKUC_003303"
     CDS             7223..8515
                     /gene="clcB"
                     /locus_tag="ACHKUC_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO6619510.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNNSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLINNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(8468..9163)
                     /gene="bioD"
                     /locus_tag="ACHKUC_003304"
     CDS             complement(8468..9163)
                     /gene="bioD"
                     /locus_tag="ACHKUC_003304"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6619511.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9288..10508)
                     /gene="mlc"
                     /locus_tag="ACHKUC_003305"
                     /gene_synonym="dgsA"
     CDS             complement(9288..10508)
                     /gene="mlc"
                     /locus_tag="ACHKUC_003305"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO6619512.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10643..>11511)
                     /gene="ynfL"
                     /locus_tag="ACHKUC_003306"
     CDS             complement(10643..>11511)
                     /gene="ynfL"
                     /locus_tag="ACHKUC_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6619513.1"
                     /translation="YFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLART
                     NRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSD
                     TLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLM
                     AMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNLTPVITQEVG
                     EAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVTIAEEDAVSEMWLVWPKHHEQSPAA
                     RNFRIHLLNALR"
BASE COUNT         2804 a         2964 c         2949 g         2794 t
ORIGIN      
        1 cagggagtga gttatgtcca aaaatgaaca aatggtgggc atcagccgca gaacactcgt
       61 taaatctacc gcgataggtt ctctggcgct ggctgcaggc ggtttttctt tgccgtttac
      121 cctgcgcagt gcagaagcag cggtacaaca ggccaacgaa aaagtggtct ggggtgcctg
      181 ttccgtcaac tgtggtagcc gctgtgcact tcgtctgcat gttaaagata atgaagtgac
      241 ctgggtggaa actgacaaca ccggcagcga cgagtatggc aaccatcagg tacgcgcctg
      301 tttgcgcggt cgctccatcc gtcggcgtat taatcatccc gatcgcctga attacccaat
      361 gaaacgcgtg ggcaaacgcg gcgaaggcaa attcgaacgg attagctggg atgaagccct
      421 ggatactatc gccagtagcc tgaaaaaaac cgtcaaacaa tatggcaatg aggctgtata
      481 tattcagtac tcttcgggga ttgttggcgg caatatgacc cgctcttctc catcagcctc
      541 ggcggtcaaa cgcctgatga actgctacgg cggttcacta aatcagtatg gctcctacag
      601 cactgcgcaa atttcctgtg ccatgcccta cacctacggc agtaatgatg gcaacagcac
      661 cacggatatt gaaaacagca agctggtcgt gatgtttggt aacaacccgg ctgaaacccg
      721 catgagcggc ggtggcatca cttatcttct tgaaaaagca cgcgagaaat cgaacgcaaa
      781 aatgattgtt atcgatccgc gatataccga tacggctgct ggtcgtgaag atgaatggct
      841 tcctattcgc ccgggcaccg atgccgcgct ggttgcgggt attgcctggg tattgattaa
      901 cgaaaatctc gttgatcaac cttttctcga taaatactgc gtcggctatg acgaaaaaac
      961 cttacccgca gatgcaccaa aaaatggtca ctataaagcc tatattcttg gtgaaggtga
     1021 tgataaaaca gcgaaaacgc cgcagtgggc ttcgcaaatt accggtatcc cggtggaccg
     1081 tatcatcaag ctggcgcgtg aaattggcac agcaaaaccc gcttatatct gccagggctg
     1141 ggggccgcaa cgccaggcaa acggtgagct gacggcacgc gctattgcta tgttgcctat
     1201 tttgacgggc aatgtcggca tcagcggcgg aaatagtggc gcgcgtgaat cgacctatac
     1261 cattaccata gaacgcctgc cggtgctgga taatccggtc aaaacgtcaa tctcctgctt
     1321 cagctggacg gatgctatcg atcatggccc gcaaatgacg gcaatccgcg acggcgtccg
     1381 gggcaaagat aaactggatg tgcccattaa gttcatctgg aactacgcgg gaaataccct
     1441 ggttaatcag cattctgaca tcaacaaaac gcatgaaatt ctgcaggacg aatcgaaatg
     1501 cgaaatgatt gtggtcatcg aaaactttat gacctcatcg gcaaaatatg ccgacattct
     1561 gctgccagac ctgatgaccg ttgagcagga agatattatt cctaacgatt acgccggtaa
     1621 catgggatat ctcattttcc ttcagccagt caccagcgaa aaattcgaac gcaaaccgat
     1681 ttactggatc ctgagtgaag tcgcgaaacg tcttggacct gacgtctatc aaaagttcac
     1741 tgaaggtcgc actcaggaac aatggttaca acatctgtac gccaaaatgc ttgccaaaga
     1801 tccggcgtta ccgtcttacg acgaactgaa aaaaatgggt atctataagc gtaaagatcc
     1861 caatggtcat tttgtcgcct acaaagcatt tcgtgacgac cccgaggcaa atccacttaa
     1921 aacgccttcc ggtaagattg aaatttattc cagcaagctg gcggaaattg cccgtacctg
     1981 ggaactggaa aaagatgaag tgataagccc actgccggtt tatgcctcaa cctttgaagg
     2041 ctgggactcc cctgaacgta gtacctttcc actgcaactg tttggtttcc attacaaatc
     2101 ccgtactcac tcgacctacg gcaacattga tgtcctgaag gctgcctgcc gtcaggaggt
     2161 gtggatcaac cctatagacg cacataaacg tgggattgcc aacggcgata tggtgcgggt
     2221 gtataaccat cgtggcgaag ttcggctacc agccaaagtg acaccacgca ttctccctgg
     2281 agttagtgct atgggccagg gagcctggca cgaggccaat atgtctggcg ataaaatcga
     2341 ccatggcggc tgtgtgaata cgctaaccac tctgcgccct tcaccactgg cgaagggaaa
     2401 cccgcagcac actaatctgg tcgagatcga aaaaatataa ccctctacaa ccataaattc
     2461 tggcatgaca ttttgtttga aaagcaataa gtgagtaatg atgaaaatcc ataccacaga
     2521 ggcgctcatg aaggctgaaa tcagccgtag aagtctgatg aaaacctccg cacttggcag
     2581 tcttgcgctg gcaagcagtg ctttcactct gccattttcc caaatggtcc gagcggcaga
     2641 gacaccggta gaagagaaag cggtctggag ttcctgcacc gttaactgcg ggagccgctg
     2701 tctgttacgt ttgcatgtga aagatgacac cgtgtactgg gtggagtctg atacgacagg
     2761 agacgacgtt tacggtaatc atcaggttcg ggcatgttta cgcgggcgct ctatccgccg
     2821 acggatgaat catcctgaca ggttgaaata tcccatgaag cgcgtcggca agcgcggtga
     2881 aggtaaattt gaacggataa gttgggatga agccctggat accatcagtg ataatcttcg
     2941 gcggatcctg aaagattacg gcaatgaggc tgtacatgtc ctgtacggaa caggcgtaga
     3001 tggcggaaac atcaccaact cgaacgtccc gtaccgtctg atgaactctt gcggtggttt
     3061 tctcagtcgc tatggcagct acagtaccgc acagatcagt gccgcgatga gttatatgtt
     3121 cggtgccaat gatggcaaca gcccggatga tatcgccaat acgaaactgg tcgttatgtt
     3181 cggaaataac ccggcagaaa cccggatgag cggcggtggt gtcacttact acgtcgagca
     3241 agcccgcgaa cgttcaaatg cacgcatgat cgtcatcgat ccacgttata acgacactgc
     3301 tgccgggcgt gaagatgaat ggctgcccat tcgccctggc accgatggcg cactggcctg
     3361 tgcgattgcc tgggtactga ttactgaaaa catggtcgat cagccatttc tcgacaaata
     3421 ttgtgttggt tacgatgaaa aaacgctgcc cgccaacgca ccacgtaacg cgcattataa
     3481 agcctatatt ctgggcgaag ggcctgacgg catagctaaa acgccggaat gggcagcaaa
     3541 aatcaccagc atcccggcag aaaaaattat ccagttggca cgagagatcg gttcagcaaa
     3601 accggcttat atttgtcagg gttgggggcc acaacgacat tccaacggcg agcaaacatc
     3661 ccgcgctatt gccatgcttt ccgttctcac cggtaacgtc ggcataaacg gcggcaactc
     3721 aggtgtacgc gaaggtagct gggatctggg agtagaatgg ttcccgatgc tcgagaatcc
     3781 tgttaaaacg cagatttccg tctttacatg gacagatgcc atcgaccacg gtacggaaat
     3841 gaccgcgacc cgtgatggtg ttcgtggaaa agaaaaactg gatgtcccca tcaagttttt
     3901 atggtgctac gccagtaaca cattgatcaa tcaacatggc gacatcaatc acacccatga
     3961 ggtgcttcag gatgacagca agtgcgagat gattgttggc attgaccact tcatgactgc
     4021 ctcggctaag tattgcgata tcctgttgcc cgacctgatg ccaacagagc aagaagacct
     4081 tatctctcat gaatctgcag ggaatatggg ctatgtgatc ctcgcccaac ccgcaacctc
     4141 agcaaaattt gaacgcaaac ccatctactg gattctgagt gaagtcgcca aacgcttagg
     4201 accagacgtt tatcaaacct ttactgaagg tcgcagtcag catgaatgga tcaaatatct
     4261 ccatgcgaaa acgaaggaac gtaaccctga gatgcccgac tacgaggaga tgaaaacgac
     4321 cgggatcttt aagaaaaaat gcccggaaga acactacgtc gctttccgcg cattccgtga
     4381 agatccacag acaaacccgt tgaaaacacc ttcggggaaa atcgaaattt attctgaacg
     4441 actggcgacg attgcggata cctgggaatt gaaaaaagat gaaattattc atccccttcc
     4501 agcttatacg ccaggttttg atggctggga cgatcccctg cggaaaacct atccactgca
     4561 gttaacgggc ttccactaca aagcgcgtac ccactccagc tacggcaata ttgatgtgtt
     4621 acagcaggcc tgcccacaag aggtgtggat caaccctatt gatgctcagg cacgcggtat
     4681 ccgtcatggc gataccgtgc gggtatttaa cactaatgga gaaatgctga ttgccgcaaa
     4741 agtgactccg cgtattctgc ctggcgttac cgccatcggg caaggtgcgt ggcttaaagc
     4801 ggatatgttt ggtgaccggg tcgatcacgg cggcagtatc aatattctga cctctcaccg
     4861 cccttcaccg ctggcaaagg gaaacccgtc gcacagcaat cttgtccaga tcgaaaaggt
     4921 ttaaggagta gcccatgacc acacaatatg gattttttat cgattccagc cgctgtactg
     4981 gctgtaaaac ctgcgaactg gcgtgcaaag attttaaaga tcttggcccg gaagtcagtt
     5041 tccgccgtat ttatgaatat gctggcggcg actggcagga agataatggc gtctggcacc
     5101 agaacgtgtt cgcttattat ctctccatct cctgtaacca ttgcgatgac cccgcatgta
     5161 caaaagtctg ccccagcggc gcaatgcata agcgtgaaga tggctttgtg gtggttgatg
     5221 aagatgtttg tatcggctgc cgctactgcc acatggcttg tccgtacggc gcaccacagt
     5281 acaatgctga aaaagggcac atgacgaagt gcgatggttg ttattcgcgc gtcgccgagg
     5341 ggaaacaacc catatgtgtc gaatcctgcc cgctgcgtgc acttgaattc ggtccaattg
     5401 aagagctgcg ccagaagcac ggcactctgg ctgccgttgc gccactgccg cgcgcgcatt
     5461 tcacaaaacc caatatcgtt atcaaaccta acggcaacag ccgcccgacc ggcgatacca
     5521 ctggttatct ggctaatccg gaggaagtgt aaaatgggaa atggatggca tgaatggcca
     5581 ctggttatct ttaccgtatt gggtcaatgc gtagtaggcg cgctgattgt tagcggaatt
     5641 ggctggttcg ccgccaaaaa tgacgcagac cgtcagcgta ttgtccgtgg catgtttttc
     5701 ctctggctgt tgatgggcat cgggtttatt gcctctgtca tgcacctggg gtcgccactg
     5761 cgggcgttta actcgctgaa tcgaatcgga gattctggtc tgagtaacga gattgctgcc
     5821 ggttccatat tttttgccgt tggcggatta tggtggctgg tggcagtcat cggtaaaatg
     5881 ccgcaagcgt tggggaaact ctggttactt gtcagcatgg cgctcggcgt cattttcgtc
     5941 tggatgatga cctgcgtgta tcaaatcgac acagtgccaa cctggcataa cggttacact
     6001 acgctggctt tcttcctgac agtattgttg agcggtccaa tacttgctgc cgcaattctg
     6061 cgggcagccc gcgttacatt taataccaca ccatttgcca tcattagcgt tctggcgctg
     6121 atcgcttgtg ccggggtgat tgtgctgcaa ggtttgtctc tggcgtctat tcactcatcc
     6181 gtacaacaag ccagcgcact ggtaccagat tacgcctcct tacaggtctg gcgtgtggta
     6241 ttgttatgcg caggtctagg atgctggctt tgtccgctca ttcgtcgccg tgaacctcac
     6301 gttgccggac ttgttctggg gttaatcctc attcttggcg gtgaaatgat cgggcgtgtg
     6361 ctcttttatg gccgcacatg acggtaggaa tggctattgc aggttaacaa attggtgcgg
     6421 ggcaacccgc acatttcagg atgttaggaa tgacccattt ttcacagcaa gataattttt
     6481 ctgtcgcggc gcgcgtgttg ggcgcgctgt tttattacgc tccagagagt gcggaagccg
     6541 cacctctggt tgcggtactc accaatgaca gctgggaaac tcagtggcct ctaccagaag
     6601 cgtcattagc gccgctggtg actgcttttc agacacagag tgaagagact cacgcccagg
     6661 cctggcagcg tttgtttgtc ggcccgtggg cactgccgtc accgccgtgg ggatcggtct
     6721 ggctggatcg cgaatctgtg ctgtttggcg attcaacatt ggcacttcgt cagtggatgc
     6781 gcgagaaagg cattcagttc gaaatgaagc aaaacgaacc ggaagatcat tttggttcac
     6841 tgctgctgat ggctgcgtgg ttggcggaga atggtcgcca gacagaatgc gaagaattgc
     6901 tggcatggca cctttttccg tggtcaacac gttttcttga cgtttttatc gaaaaagcag
     6961 aacacccttt ctaccgtgca ctgggtgaac tcgctcgctt aacgctggcg caatggcaat
     7021 cacaactgtt gattcctgtc gcggttaaac cgctgtttcg ataggatcac cgtattattg
     7081 ccggatggtg acgattacac gcaattcggc aattaatagg ttacagtgtc acgtttttta
     7141 tctcttaaag cacgcactgc ttttgcggct ggcctctttt gccgcaaaat agtcgcccgt
     7201 gtttcattgc ccatttctgc tcatgcatca tctacacatc tatccggatc tgcgcactat
     7261 gttccgccgt ctgcttatcg caacaatcgt cggtattctt gcggcctttg ccgttgccgg
     7321 gtttcgtcat gcgatgctgc tactggagtg gctgttcctc aataataact ccggcagtct
     7381 ggtcaatgca gcgacaaatc tttccccgtg gcgacggctg ctaaccccgg cgctcggcgg
     7441 actggcggcg ggtttgttgc tgatgggctg gcagaaattt acccagcaac gccctcatgc
     7501 gccgaccgat tacatggaag cgttgcaaac cgatggtcag tttgattacg cagcaagcct
     7561 ggttaaatcg cttgcctctc tactggtagt aaccagcggc agtgcaattg gtcgcgaagg
     7621 tgcgatgatt cttttagctg cccttgcagc ctcgtgtttt gcccaacgtt ttacgccacg
     7681 ccaggagtgg aaattatgga tcgcctgtgg agctgcggcg ggaatggctg cggcctatcg
     7741 cgccccgctt gctggcagtt tatttatagc cgaagtgctg tttggcacta tgatgttggc
     7801 ctctctcggc ccggtgatta tttccgccgt cgtggcgttg ctgattaaca atcttattaa
     7861 tcatagcgac gcattactct acagcgtaca actctcagtg acggttcagg ctcgtgacta
     7921 tgcgctgatt atcagtacag gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt
     7981 aatgaacgcc tgtcatcgtg gatttgtgag tctcaagctt gcgccgccct ggcaactggc
     8041 actgggcggg ttgatcgtgg gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg
     8101 ctatagcacc gtacaatcct ttttaaccgc tccgccactg ttaatgatca ttgccgggat
     8161 cttcctctgt aaactgtgtg ccgtgctggc gagtagtggt tctggcgcgc ccggtggtgt
     8221 ctttacaccg acgctattta tcggtcttgc cattggcatg ttgtatggtc gtagcctggg
     8281 attatggtta cctgatggcg aagaaattac gcttttactc ggattgacag ggatggcgac
     8341 actgttggcg gcgaccacgc acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac
     8401 cggggagtat cagctactcc ccggtttatt gattgcctgc gtaattgcgt cggtaatttc
     8461 gcggacgtta caccgtgact ctatctaccg ccagcacact gcgaagcata gctaagcgga
     8521 tgtattgccc cagttcacgc tgttcggcgc gcggcagata aggcagttca ccaatgagcg
     8581 gtgccggaag ttttttaccg agcacatcaa tgatttccgc ataatgcgcc agtcctgggt
     8641 tgattcggtt agccacccag ccaatgagcg gcagcccgtc gttggcaatc gcctgagctg
     8701 ttagcagtgc atggttaatg caaccttcct gaataccgac aaccatcaac accggcagtt
     8761 gttcctgcac cacccattca gagagcggac gcaaatcatt catcagactg cgccagccgc
     8821 cagtcccttc taccacgaca tgatcgactt tatcggtcag gtttgccagg ccgtttgaaa
     8881 tgagggtgta attgattggg caactgtgcg ccacgctact ttcttcttcg cttaacgcga
     8941 taggattaac tgcttcataa ggcagttcga tggttgaaac actctgcaac accagggcat
     9001 ctttattacg cagcccttcg ggtgtctctt tgctcccctt cgctacgggt ttatatcccg
     9061 caaccgtttt tccctgggag gctaacgctt gtagcaatgc gcgggaaacc accgttttcc
     9121 ctacagaagt gtctgtaccg gtaataaaga aacgcttcag catcactaac tccaccgtta
     9181 tgcttcacaa atataaacca ggaaaataat taaccttgaa agtctaagtt atgctttcct
     9241 ggcccaaatt gagatagcgc aaattttggt agaacagtta aaaaatgtta accctgcaac
     9301 agacgaatca acaaagaacc gttatacatc gcgtctttta ccagcgcagc gcctgccatc
     9361 gtgccctggt tagaaaactg agtactctca acgctaatgt gctgactata cgcaggaagg
     9421 gcctgctgac ggatgctgtc cgagatgact gggaagagga tatctgccgc tttacttaac
     9481 ggtgagccaa tcagtatttt ttgtgggtta aataaattca ccatgatggc aagaatgcga
     9541 ccgacatgcg cgcctacccc ggtaatgatg tcttttgcca gtagatcgcc gcgcaatgcc
     9601 gcctgacaca atgagtccac ggttaacggt tgtccatgta acatcgagct catggattga
     9661 ttgagacgca gctgtgccag ctcaagaata ctgtccacac tggcgatggt ttcgaggcaa
     9721 ccgtgattcc cgcaataaca gcgtttccca tacgggtcga cctgtgtgtg gcctatttcc
     9781 acgaggctac tgctgccggc gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg
     9841 ttgtgatcga taaccacctg aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc
     9901 tctgccatcg tccatgcgct gatatcatgc tggatataaa ccggtacgcc ggtatgctgc
     9961 tccagcgcct cgccgagcgg catctctttt acatcctcgt agaacggcat gcgatgtaca
    10021 atgccatttt ccgtatcaat aattcccggc aaggttatgg caatcgaagt cagacgctca
    10081 agttttttct ggtggcggat aaaaaactga tcgatatggg aaatgatacg atccagcaat
    10141 ggcgagtcat cttttaacgc cagttcctgc gactcttcca ccaccagttt gctgctcaga
    10201 tcgcgcagag caaggaaaat ctccccgcga ctaatgcgca gagaaagata gtgccaggct
    10261 tcagtttcaa ccaccagccc caccgccgga cggccacggt tccccgcttc tttgatttcc
    10321 agctcttgca ccaggtgtgc ttcgagcatc tcacggacaa ttttagtgat actggcagga
    10381 gccagttgcg ccagacggga aagatcgata cgcgagactg gaccaagctg atcaatcagg
    10441 cgataaaccg cgcctgcgtt ggtctgcttt atttgatcaa tgtgcccagg ctgattttca
    10501 gcaaccaccg catactccct atattttcgc gctccgaaat aatctgtagg ctatggtgaa
    10561 gcacttcaat acgtgtcgtc aaatttttac ttaggcatgt gattaacagc acatttttcg
    10621 ggctttttcg ctgaaatttc cctcacctga gagcattcag cagatgaata cgaaagtttc
    10681 gcgcagctgg actttgttca tgatgtttcg gccagaccaa ccacatttca gaaaccgcat
    10741 cctcttcagc aatcgtcacc cagcgcattt cgttgagctg aacacgttta aatgacgcag
    10801 gcaaaattga aacacccaga ccggcggaaa ccagaccgat gatggtcatt gcctcaccca
    10861 cctcctgagt gatgacgggc gtcaaattgt aacgtctcat cagcccgaga atatcgtcat
    10921 acagccctgt tccgacgtgc ggatcaaaaa agacaaaggg ttctttcgcc agttcagcca
    10981 gcgttacact cgggttattt gccaggggat gatcgtgcgg gatcatcgcc ataagcggtt
    11041 catggacgat gactgcgtgt tcaagcgtct ccggtaacgc tgtgttacgc agcaatccca
    11101 tatccagcgt tccttcaatg agcggagcga tttgctcgcg agtgttcatt tcgcgggttt
    11161 gtaaatggac atcgggataa tcacggcgaa acagcgataa cgtatcggac accgcccgaa
    11221 taaaaggagc ggacgaagta aaaccaatgc gcaactcccc cgcttcgccc tgatgcagcc
    11281 tttcagcgcg agcggcagca tcatccacca tagacaggat ttgccgacta tctacaagaa
    11341 actgttttcc tgcttccgtc agcaatacac tgcgattggt ccgtgccagc agtcgggcac
    11401 caatttgttg ctccagcgcc tgaatctgct gacttagagg tggttgcgaa atattcaggc
    11461 gggcagcggc acgcccgaaa tgcagctctt ctgcaacagc gacaaagtaa c
//
LOCUS       DAZFRG010000154        11452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFRG010000154 DAZFRG010000000
VERSION     DAZFRG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.154"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..986)
                     /gene="pyrD"
                     /locus_tag="ACHKUC_003307"
     CDS             complement(<1..986)
                     /gene="pyrD"
                     /locus_tag="ACHKUC_003307"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO6619514.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGATLVQIYSGFIFKGPPL"
     gene            1223..1798
                     /gene="ssuE"
                     /locus_tag="ACHKUC_003308"
     CDS             1223..1798
                     /gene="ssuE"
                     /locus_tag="ACHKUC_003308"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO6619515.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQFIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1791..2750
                     /gene="ssuA"
                     /locus_tag="ACHKUC_003309"
     CDS             1791..2750
                     /gene="ssuA"
                     /locus_tag="ACHKUC_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO6619516.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPPTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2747..3892
                     /gene="ssuD"
                     /locus_tag="ACHKUC_003310"
     CDS             2747..3892
                     /gene="ssuD"
                     /locus_tag="ACHKUC_003310"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO6619517.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVWAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3904..4695
                     /gene="ssuC"
                     /locus_tag="ACHKUC_003311"
     CDS             3904..4695
                     /gene="ssuC"
                     /locus_tag="ACHKUC_003311"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO6619518.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4692..5459
                     /gene="ssuB"
                     /locus_tag="ACHKUC_003312"
     CDS             4692..5459
                     /gene="ssuB"
                     /locus_tag="ACHKUC_003312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6619519.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(5654..8266)
                     /gene="pepN"
                     /locus_tag="ACHKUC_003313"
     CDS             complement(5654..8266)
                     /gene="pepN"
                     /locus_tag="ACHKUC_003313"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO6619520.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTAVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8532..9734
                     /gene="pncB"
                     /locus_tag="ACHKUC_003314"
     CDS             8532..9734
                     /gene="pncB"
                     /locus_tag="ACHKUC_003314"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO6619521.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPKADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9903..11303
                     /gene="asnS"
                     /locus_tag="ACHKUC_003315"
     CDS             9903..11303
                     /gene="asnS"
                     /locus_tag="ACHKUC_003315"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO6619522.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2617 a         2960 c         3035 g         2840 t
ORIGIN      
        1 atcagcggcg ggcctttaaa aataaaacca gaataaattt gcaccagtgt ggcacccgca
       61 gcaatctttt cacgcgcagc gataaccgag tcaatgccgc caacaccaat aatcggcaag
      121 cgtccgttta attcctgtga caagcggcga ataatttcgg tgctttttaa ctgaagcgga
      181 cgaccactta agccaccggt ttgatcacaa tttttcattc cctgaaccag agaacgatcg
      241 agtgtggtat tggttgcaat aacgccatca atattatggc gaactaaact atcggcaacc
      301 tggatcaatt cttcttcaga aagatccggc gcgatcttca ctgcgatcgg cacatattta
      361 tggtgcatca cttgcaaatc attttgttta tttttaatcg cagttaagag atcatccagc
      421 gcttcaccat attgcagtgt gcgtaatcct ggggtatttg gcgatgaaat attgatggcg
      481 atatatcccg cataggcata gattttttcc atacaaatca gatagtcatc tttaccctgc
      541 tccaccggcg tatctttatt tttgccgatg ttaataccca gaacgccgtc ataatgggct
      601 ttttttacgt tctctacgag gttatcaacg ccaagattat taaagcccat acggttgatc
      661 aaaccttcgg catctaccag acgaaagaga cgcggcttgt cattacctgg ctggggacgt
      721 ggcgtgacgg taccgatctc gatggatcca aatcccatcg cgcctaacgc gtcaatgcac
      781 tccccgtctt tatcaagacc ggctgccaga ccaagcggat ttttaaacgt caggcccatg
      841 cagttaacag gtttcgcagg cactttctgc cgcaccagtg cttcaaacgg cgttcctgta
      901 atacggcgta attgctgaaa agtaaactca tgagcgcgct ctggatcgag ctggaaaagg
      961 gctttacgaa cgaaggggta gtacatgaac tctcctggat tcccggtgtg caaaccgggg
     1021 gcgtattatg tgcgagttcg gaccaaaagg gaattgacct gcggcaataa atagcaaacg
     1081 ttttcttatt gcgctttttt tatgcttttt ttggcttttc ttcctcagat aaatcatttc
     1141 gactcgcgga cgccctctgt cagactgacc gaattgttat caatgttaac aaaacaagaa
     1201 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
     1261 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgtaga
     1321 ggtttatcac tggaatttgc aaaacttcgt cccggaagat ctgctttatg ctcgtttcga
     1381 tagtccggca ctcaagacct tcaccgaaca gctgcaacag gccgatgggc tgattgtcgc
     1441 cacgcctgtg tataaagccg cctattccgg tgcactgaaa accctgctcg acctgctgcc
     1501 agaacgtgcg ttgcaaggca aagtggtgct gccgctggcg acaggcggca ccgtggccca
     1561 tctgctggcg gtcgattatg cccttaagcc agttttaagt acgctgaaag cccaggagat
     1621 cctgcacggc gtgtttgccg atgactcgca attcattgat tatcaacaca aaccccagtt
     1681 cacgccaaat ctgcaaactc gtcttgatac cgcgctagaa actttctggc aggcgttgca
     1741 ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaga
     1801 tcattaaact ggcgctggcg ggtttactta gcgtctctac acttgctgtt gccgcggagt
     1861 cttcgccaga ggcgttacgt ataggctatc aaaaaggcag tattggtatg gtgctggcaa
     1921 aaagccacca gttactggaa aaacgctatc cgcaaacaaa aatctcctgg gtggagttcc
     1981 ccgctggccc acagatgctg gaggcgttaa acgttggcag tatcgatctc ggcagtaccg
     2041 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatac gtgggcgtcg
     2101 agccaccgaa acccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
     2161 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttcg cacaatcttt
     2221 tactgcgagc ccttcgccag gccgggctta aattcacgga tattcagccc acttacctga
     2281 cgcctgccga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     2341 atccctacta ctccgctgca ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg
     2401 atctcaatca aactggatcg ttttatctgg cagctcgtcc gtatgcagaa aaaaacggcg
     2461 cttttattca gggtgtgctg acaaccttta gtgaggccga tgcgttaacc cgcagccagc
     2521 gcgaacaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gttattgcct
     2581 catatctgga tcatcgtcct ccccccacca tcaaaccgtt gagcgctgaa gttgccgcct
     2641 tacagcagca aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa aaagtcgata
     2701 ttcgccagcg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     2761 gttctggttt ttaccgaccc acggtgacgg gcattatctg ggaacggaag aaggttcacg
     2821 tccggttgat cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac
     2881 cggtgtgctg atcccgacgg ggcgatcgtg tgaagatgcg tggctggtgg cggcgtcgat
     2941 gatcccggtg acacaacggc tgaagtttct tgtcgccctg cgccccagcg taacctcacc
     3001 taccgttgcc gcccgtcagg ccgccacgct tgaccgtctc tctaacggac gtgcgttgtt
     3061 taacctggtc acaggcagcg atccacaaga actggcaggc gacggcgtgt tccttgatca
     3121 tagcgagcgt tacgaagcct cggcagaatt tacccaggtc tggcggcggt tgttgcttgg
     3181 cgagactgtg gatttcaacg gcaaacatat tcatgtacgc ggagccaaac tgctcttccc
     3241 gccgattcaa cagccttatc ctccgcttta ctttggcgga tcgtcagatg tcgcccagga
     3301 gctggcggca gaacaagttg atctctacct tacctggggc gaaccaccgg aactggtaaa
     3361 agagaaaatc gaacacgtgt gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg
     3421 tattcgtctg catgtgattg ttcgtgaaac caacgacgaa gcgtggcagg ccgccgagcg
     3481 gttaatctcg catcttgatg atgaaactat cgccaaagca caggccgcat tcgcccgcac
     3541 ggattccgta ggacaacagc gaatggcggc gctacataac ggcaagcgcg acaatctgga
     3601 gatcagcccc aatttatggg cgggtgttgg cttagtgcgc ggcggtgccg ggacggcgct
     3661 ggtgggcgat ggtcctacgg tcgccgcgcg aatcaacgaa tacgctgcac ttggcatcga
     3721 cagttttgtg ctttcgggct acccgcatct ggaagaagcg tatcgggtcg gcgagttact
     3781 gttcccacat ctggatgtcg ccatcccgga aattccccag ccacaaccgc tgaatccaca
     3841 aggcgaagcg gtggcgaatg attttatccc ccgtaacgtc gcgcaaagct aaggaacacg
     3901 atgatggcaa cgccagtgaa gaagtggtta ttgcgcgttg ctccctggtt tttaccggtg
     3961 ggcatcgtgg cggtgtggca actggcctcc tcggttggct ggctatcgac gcgtattttg
     4021 ccctcaccgg aaggagtggt gatggcgttc tggacgctct ctgccagcgg cgaactgtgg
     4081 cagcatctgg cgatcagctc ctggcgtgcg ttgattggct tttcaattgg cggatcactg
     4141 ggattaattc tggggctgat tagcgggctg tcacgctggg gagaacggct gctggatacc
     4201 tcaattcaga tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg
     4261 ttcggcattg atgaatccgc taaaatattt ctggtggcgc tcggtacgct attccccatt
     4321 tatatcaaca cctggcacgg catccgtaat atcgaccgtg ggctggtgga gatggcacgt
     4381 agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg tgccctgccc
     4441 tcaattatgg tcggtgtgcg ttttgctttg ggcctgatgt ggctgacgct gatcgttgcc
     4501 gaaactattt ctgccaattc cggcattggt tatctggcga tgaatgcgcg ggagtttctg
     4561 caaacggacg tggtggtagt cgccattatt ctttacgccc tgctcggcaa acttgctgac
     4621 gtcagcgcgc agctgttaga gcgtctctgg ttgcgctgga acccggctta tcatttgaag
     4681 gaggccacgg tatgaatact gctcgtctga accagggcac accattgttg ctcaatgcag
     4741 taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     4801 gtcagtttgt ggcggtggtg ggccgcagcg gcggtggcaa aagtaccctg ctgcgcctgc
     4861 tggcaggtct ggaaacgcca accgctggcg atgtgttagc gggcaccaca ccgctggctg
     4921 agattcagga tgatacgcga atgatgtttc aggacgcgcg tctgctgccg tggaaatcgg
     4981 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgcggcg cgtcaggctc
     5041 tggctgcggt agggctggag aatcgcgcgg gggaatggcc tgccgcactt tctggcgggc
     5101 agaaacaacg tgtggcgctg gcaagggcgc tgattcatcg accgggttta ttgttgcttg
     5161 atgaaccact cggtgcgctg gacgctttaa cgcgactcga gatgcaggat ttgattgtgt
     5221 cactctggca ggagcacggc tttaccgttt tgctggtgac gcatgatgtg agcgaagcgg
     5281 tggcgatggc tgaccgggtg ttgttaattg aagagggaaa aattggtctg gatttgacgg
     5341 tggatatccc ccgtccgcgc cggttagggt cggtgcgatt agcggagctg gaagcggaag
     5401 tgttgcaacg ggtgatgcaa cgtgggcact ctgaacaacc gattcgacgt catggttaat
     5461 atgccggatg tggcgcacaa aaccagcatt ttcaacggga ttgtaggcct gataagacgc
     5521 gtcaagcgtc gcatcaggca gttgggcacg actgcctgat gcggcgtgaa cgccttatcc
     5581 agcctacaaa accagcaatt tcaacggact tgtaggcctg ataagacgcg tcaagcattt
     5641 tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc gtacagatcg ccagagagat
     5701 tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt ttggcatcgt
     5761 aacgtttcag gcgaatcagc ggctcaatca ggcgtgaagc cacctgcggg ttacggctgt
     5821 tgaggtcggt aagcatttcc accaggaact gataaccgct gccatcttcg gcatggaacg
     5881 ctgccgggtt gctgcccgca aatgcgccaa tcaatgaacg aatgcggttc gggttgctca
     5941 tggtaaatga gcgatgctgc aacagaccac gtaccgtctc cagcacattc gccgccgggc
     6001 tggtggcttg caggataaac catttatcca tcaccagacc atcctgatgc cacttgtcgt
     6061 cgtactcctg catcagcgcg tcacggcatg gcagttgtgc agcaaccgcc gcagaaagcg
     6121 ccgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc agcacgtcag
     6181 ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga gtgcgttttg
     6241 caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa atagccagta
     6301 gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact tcagcaatag
     6361 caatcgggtc gatgatatcg aacagttctg ccatttcatt gacggaaggc agcgtcagga
     6421 tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg aaagcgtcag
     6481 ccacatgcac cggcagagac aacggctgcc cttgctgatg acgggcgacg ttcagcttga
     6541 tgtaggttgc cagcaaactt tgcgccgcgt cccagcgaga gaaatcatta cgcgcatgac
     6601 gcatcaggaa ggtcagttgc tgatcgctcc acttatattc cagtttcact ggcgcagaga
     6661 attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca aaggtctgtt
     6721 ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc aacgggatca
     6781 ctttgccttc gttgtcatac agttcgatgg caaacggaat atgcagtggt tgtttttctg
     6841 cctgatccgg tgtagctggc gtgcgctggc tgatggtcag ggtgtactgc tcagtttccg
     6901 gattgtagtc gtctttgacg gtcacaaccg gcgtgccgga ctggctgtac caacggcgga
     6961 aatgggaaag atcgacattc gacgcatctt ccatcgcctg cacaaaatcg tcacaggtcg
     7021 ctgcgctacc atcatgacgc tcgaaataga gctgcatccc tttctggaaa ttttcttcgc
     7081 caagcagagt gtggatcata cgaatcactt ccgcgccctt ctcgtaaacg gtcagggtgt
     7141 agaagttgtt catttcaatg accatatccg ggcgaattgg gtgcgccatc gggctggcgt
     7201 cttctgcaaa ctgcaatccg cgcatggtgc gcacattatt gatacggttt accgcgcggg
     7261 aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct ttcagactga
     7321 gctggaacca gtcgcggcag gtgacacggt tgccggtcca gttatggaaa tattcatggc
     7381 cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc ggtgtcggtg cgggccagca
     7441 catatttgga gttaaagata ttcagcccct tattctccat tgcgcccata ttgaagaaat
     7501 ccaccgcgac gatcatatag atgtcgaggt catactccag gccgaagcgt tcttcatccc
     7561 atttcatgga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg ttgccacgat
     7621 cgacgtacag ctccagcgct acttcgcgac cagaacgcgt ggtgaaagtg tcgcgcagca
     7681 catcaaagtc gcctgccacc aacgcaaaca ggtaacacgg tttcgggaac gggtcctgcc
     7741 actgtaccca atgacgtcca ttttccagtt caccttgcgc aacgcggtta ccattggaaa
     7801 gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc gccagcacat
     7861 ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa
     7921 gcgcatcgcc tgactgataa agcccttcca gcgcagtatt cgccgccggg ctaatttcat
     7981 taacgatctt aagcgtaaaa cgctccggca aattactgat gaccagtgcg ccctcttctt
     8041 ctttccaggc ggtccacggc tcatcattaa tataaacaga aaccagtttg aggtcttcgc
     8101 cgttgagacg aagcggaaca tctgatgcac catgacggac agcctggctg accgcggtaa
     8161 cggccgtctt ttgcgcgtcc aggtcaaagg tcaagtcaat atcagtaatc tggtaatccg
     8221 gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat aacctttagc
     8281 atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat
     8341 cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata caccgcgtta
     8401 tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt gcgaaacaag
     8461 gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta cgaggacgct
     8521 actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg gatacagatg
     8581 cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg
     8641 cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct attcgtgaac
     8701 aggttcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg ctctctgccc
     8761 tgcctttctt taaggccgac tatcttaact ggctacgcga gttccgcttt aacccggaac
     8821 aagtcaccgt gtccaacgat aatggcaagc tggatattcg tttaagcggc ccgtggcgtg
     8881 aagtcatcct ctgggaggtt cctttgctgg cggttatcag tgaaatggta catcgctatc
     8941 gctcaccgaa ggccgacgtt gcgcaagccc tcgacacgct ggaaaacaaa ttagtcgact
     9001 tctcggcatt aaccgccggt cttgatatgt cgcgcttcca tctgatggat tttggcaccc
     9061 gccgccgttt ttctcgcgaa gtacaagaaa ccatcgtgaa acgtctgcaa caggaatcct
     9121 ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa
     9181 cacaggcaca cgaatggttc caggcgcatc agcaaatcag cccggatcta gccaacagcc
     9241 agcgagctgc acttgctgcc tggctggaag agtatcccga ccaacttggc attgcattaa
     9301 ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc gccagtcgct
     9361 atcagggcct gcgccatgac tctggtgacc cggttgaatg gggtgaaaaa gccattgcac
     9421 attatgaaaa gctgggaatt gatccacaga gtaaaacgct ggttttctct gacaatctgg
     9481 atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg
     9541 gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acctctgaat attgtcatta
     9601 agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct ggaaaaacta
     9661 tctgccacga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta
     9721 aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc tttaattccg
     9781 aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag gtaacatagg
     9841 tatcccccca tttacgggtg gacaagtgtt tattttttcc gactattaac agagagaata
     9901 ttatgagcgt tgtgcctgta gccgacgtac tccagggccg cgtagccgtt gacagcgaag
     9961 tcaccgtgcg cggatgggta cgtacccgcc gagattcaaa agctggcatc tccttcctcg
    10021 ccgtttatga cggttcctgc tttgatcctg tacaggctgt catcaataat tctctgccca
    10081 attacaatga agacgtcttg cgtctgacca ccggctgctc ggtcattgtg acgggtaaag
    10141 tcgtggcgtc gccgggccag gggcaacaat ttgaaattca ggccagcaag gttgaagttg
    10201 ctggttgggt tgaagatcca gacacttacc cgatggcggc aaaacgccac agcattgagt
    10261 atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc gtcgcgcgcg
    10321 ttcgccatac gctggcgcag gcgctgcatc gcttctttaa cgagcaggga ttcttctggg
    10381 tttcaacgcc actgattacc gcatctgata ccgaaggtgc tggcgaaatg ttccgtgttt
    10441 caacgctgga tctggaaaac ctgccgcgta acgatcaggg caaagtggat ttcgacaaag
    10501 acttctttgg taaagagtct ttcctgaccg tatcgggcca gttgaacggc gaaacctacg
    10561 cttgcgcatt gtccaaaatt tataccttcg gcccgacttt ccgtgctgaa aactccaaca
    10621 ccagccgtca cctggcggaa ttctggatgc tggagccgga agtggcgttt gctaacctga
    10681 acgatatcgc aggtctggct gaagccatgc tgaaatatgt cttcaaagcg gttctcgaag
    10741 aacgcgctga cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc gtttcacgtc
    10801 tggaacgctt cattgaggcc gatttcgcgc aggtggatta caccgacgca gtgaccattc
    10861 tcgaaaactg cggcaggaag tttgaaaacc cggtttactg gggcgtcgat ctctcttctg
    10921 agcatgagcg ttatctggcg gaagaacact ttaaagcacc ggtagtggtt aaaaactatc
    10981 cgaaagatat taaagcgttc tatatgcgcc ttaacgaaga cggtaaaacc gttgcggcta
    11041 tggacgttct ggctccgggc atcggtgaga tcattggtgg ctcccagcgt gaagaacgtc
    11101 tggacgtgct ggacgagcgt atgcttgaaa tgggcctgaa caaagaagat tactggtggt
    11161 atcgcgatct gcgtcgctac ggtactgttc cgcattcagg tttcggtctt ggttttgaac
    11221 gcctgattgc ttacgtaact ggcgtgcaaa acgtacgtga tgttattccg ttcccacgta
    11281 ctccgcgtaa cgccagcttc taatttagcg tcttcaagag ccagcgcccg ctggctcttt
    11341 ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag caatctattt gcaaccccgc
    11401 cataacgatc atcctgttac ggaatattac gctgcaacat ttacgcacaa aa
//
LOCUS       DAZFRG010000155        11380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFRG010000155 DAZFRG010000000
VERSION     DAZFRG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.155"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..1016
                     /locus_tag="ACHKUC_003316"
     CDS             99..1016
                     /locus_tag="ACHKUC_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6619523.1"
                     /translation="MPAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSAVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDREICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRQI
                     IDLAQRCGALAVTAKGAMTALPCRQELEW"
     gene            1085..2332
                     /locus_tag="ACHKUC_003317"
     CDS             1085..2332
                     /locus_tag="ACHKUC_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO6619524.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDQEHQCVAADVGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2412..2564)
                     /locus_tag="ACHKUC_003318"
     CDS             complement(2412..2564)
                     /locus_tag="ACHKUC_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619525.1"
                     /translation="MLSLMNSQARDTRDRSLNYDNALEISEPCIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(2772..2846)
                     /locus_tag="ACHKUC_003319"
     tRNA            complement(2772..2846)
                     /locus_tag="ACHKUC_003319"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2811..2813),aa:Arg,seq:cct)
     gene            complement(2922..3854)
                     /gene="yfdC"
                     /locus_tag="ACHKUC_003320"
     CDS             complement(2922..3854)
                     /gene="yfdC"
                     /locus_tag="ACHKUC_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO6619526.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSVLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNIIGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKVEARQKAERAENIKKNDKNPA"
     gene            4148..4903
                     /gene="mlaA"
                     /locus_tag="ACHKUC_003321"
     CDS             4148..4903
                     /gene="mlaA"
                     /locus_tag="ACHKUC_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO6619527.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4965..6305)
                     /gene="fadL"
                     /locus_tag="ACHKUC_003322"
     CDS             complement(4965..6305)
                     /gene="fadL"
                     /locus_tag="ACHKUC_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO6619528.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVAPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6677..6961
                     /gene="yfcZ"
                     /locus_tag="ACHKUC_003323"
     CDS             6677..6961
                     /gene="yfcZ"
                     /locus_tag="ACHKUC_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO6619529.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            7155..8465
                     /gene="fadI"
                     /locus_tag="ACHKUC_003324"
     CDS             7155..8465
                     /gene="fadI"
                     /locus_tag="ACHKUC_003324"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO6619530.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            8465..10609
                     /gene="fadJ"
                     /locus_tag="ACHKUC_003325"
     CDS             8465..10609
                     /gene="fadJ"
                     /locus_tag="ACHKUC_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO6619531.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKVEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADIKMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPTTERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCNRLVEMSERGESFWKTTATDLQ"
     gene            10812..11297
                     /gene="sixA"
                     /locus_tag="ACHKUC_003326"
     CDS             10812..11297
                     /gene="sixA"
                     /locus_tag="ACHKUC_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO6619532.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2735 a         2637 c         2988 g         3020 t
ORIGIN      
        1 ttttgtgatc gaatatgcat gttttagtaa atccatgacg attttgcgaa aaagaggttt
       61 atcactatgc gtaagtcaga tgaatttgag ggaaaaaaat gccagccaaa gtatgggttt
      121 taggggatgc ggtcgtagat ctcttgccag aatcagacgg gcggctactg ccttgtcctg
      181 gcggtgcgcc agctaacgtt gcggtgggaa tcgccagatt aggcggaata agtgggttta
      241 taggtcgggt gggggatgat ccttttggtg cgttaatgca aagaacgctg ctaactgagg
      301 gagtcgatat cacgtatctg aagcaagatg aatggcaccg gacatccgcg gtgcttgtcg
      361 atctgaacga tcaaggggaa cgttcattta cgtttatggt ccgccccagt gccgatcttt
      421 ttttagagac gacagacttg ccctgctggc gacatggcga atggttacat ctctgttcaa
      481 ttgcgttgtc tgctgagcct tcgcgtacca gcgcatttac agcgatgacg gcgatccggc
      541 atgccggagg ttttgtcagc ttcgatccca atattcgtga agatctatgg caagacgagc
      601 atttgctccg cttgtgtttg cggcaggcgc tacaactggc ggatgtcgtc aagctctcgg
      661 aagaagaatg gcgacttatc agtggaaaaa cacagaacga tcgggagata tgcgcgctgg
      721 caaaagaata tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct
      781 gttatcgagg acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg
      841 gggcgggaga tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta
      901 cagatgaaag agaaatgcga caaattatcg atctcgctca acgttgcgga gcgcttgcag
      961 taacggcgaa aggggcgatg acagcgctgc catgtcgaca agaactggaa tggtgagaag
     1021 taaacggcga agtcgctctt atctctaaat aggacgtgaa ttttttaacg acaggcaggt
     1081 aattatggca ctgaatattc cattcagaaa tgcgtactat cgttttgcat ccagttactc
     1141 atttctcttt tttatttcct ggtcgctgtg gtggtcatta tacgctattt ggctgaaagg
     1201 acatctaggg ttgacaggga cggaattagg tacactttat tcggtcaacc agtttaccag
     1261 cattctattt atgatgttct acggcatcgt tcaggataaa ctcggtctga agaaaccgct
     1321 catctggtgt atgagtttca tcttggtctt gaccggaccg tttatgattt acgtttatga
     1381 accgttactg caaagcaatt tttctgtagg tttaattctg ggtgcgctct tttttggtct
     1441 ggggtatctg gcgggatgcg gtttgcttga cagcttcacc gaaaaaatgg cgcgaaattt
     1501 tcatttcgaa tatggaacag cgcgcgcctg gggatctttt ggctatgcta ttggcgcatt
     1561 ctttgctggc atatttttta gtatcagtcc ccatatcaac ttctggttgg tctctctatt
     1621 tggcgctgta tttatgatga tcaacatgcg ttttaaagat caggaacacc agtgcgtagc
     1681 ggcggatgtg ggaggggtaa aaaaagagga ttttatcgca gttttcaagg atcgaaactt
     1741 ctgggttttc gtcatattta ttgtggggac gtggtctttc tataacattt ttgatcaaca
     1801 actctttcct gtcttttatg caggtttatt cgaatcacac gatgtaggaa cgcgcctgta
     1861 tggttatctc aactcattcc aggtggtact cgaagcgctg tgcatggcga ttattccttt
     1921 ctttgtgaat cgggtagggc caaaaaatgc attacttatc ggtgttgtga ttatggcgtt
     1981 gcgtatcctt tcctgcgcgc tattcgttaa cccctggatt atttcattag tgaagctgtt
     2041 acatgccatt gaggttccac tttgtgtcat atccgtcttc aaatacagcg tggcaaattt
     2101 tgataagcgc ctgtcgtcga cgatctttct gattggtttt caaattgcca gttcgttggg
     2161 gattgtgctg ctttcaacgc cgactgggat actctttgac cacgcaggct accagacggt
     2221 ttttttcgca atttcgggta ttgtctgcct gatgttgcta tttggcattt tcttcctgag
     2281 taaaaaacgt gagcaaatag tgatggaaac gccagtacct tcagcaatat aaacgtaaac
     2341 tttttccggt tgttgtcgat agctctatat ccctcaaccg gaaaataata ataccaaaat
     2401 gcttagccct gctaataatc gcctaatcca aacgcctcat tcatgttctg gtacagtcgc
     2461 tcaaatgtac tccggataca cggctcgctg atttccaggg cattgtcgta attcagcgac
     2521 ctgtcccgtg tatcacgggc ctgcgaattc atcaaggaaa gcattgtgga gtgaagtatc
     2581 aagtcacgcc atatttcgcg atcaggattg tgtgtgatgg ttacatcgcc cagcccaggg
     2641 ctgtttagtc atcagcgttt tctgaccgtg ttgagatttc aacctgttgc aggaaaaatg
     2701 agtagatagg gcaaatgtgc tgccaaactt atcttttacg gggtgaaggt agatatcgtt
     2761 tgaagggtat ctggtgtccc ctgcaggaat cgaacctgca attagccctt aggaggggct
     2821 cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta atggactacg
     2881 gggtaagtac acagccgctc gattgcttaa accctcgcca tttatgccgg gtttttatca
     2941 tttttcttaa tgttttccgc acgttctgct ttttgacgtg cttctacttt gcgcttgttg
     3001 ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt gccgccgcag
     3061 atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca gtgcagcgta
     3121 ccgttaaaca ccagatagag gatttctaca gaaccgacaa cgatgtgggt ggtgtcaccc
     3181 agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc cgctgcagga
     3241 aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc aaacatctcg
     3301 ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg agtttcttca
     3361 ttgaagatag gcatatattc aaatgcccac gcagcaatac ctgtcccgat aatattaccc
     3421 agcagcacga tgccccataa ccgcataagt aagccgacgt tgctcattgt cggtttttgc
     3481 atgacgggta gtaccgcagt tacggtgttt tcggtaaata attgctggcg ggccattatg
     3541 acgataataa aaccaaaggt ataaccgaga ttctccagca agacgctgcc cggcacgcct
     3601 tccagttcga catgaaatat cccttttgcc agtagcgaag cgcccatcga caggcccgcc
     3661 gcaatggctg accacagtag cgccattgcg tcgcgttcca gctctttttc accatcctgg
     3721 cggatatgct catgaattgc catcgcccgg gaggggagtc ggtcttcatc tatttctatt
     3781 tttttaccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg ttgatcaatt
     3841 ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc gtagcggctt
     3901 ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggccgcg tttgcctttt
     3961 tctgtttcta ttgaatcaag tagcctacag ggcggcgatt accaggctat gatcaaatca
     4021 gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac acttaaacag
     4081 gaggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa aaaacaggga
     4141 gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc tggtggggtg
     4201 tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt ttaaccgcac
     4261 tatgtacaac ttcaacttca atgtattaga cccgtatatt gttcgaccgg tcgctgtcgc
     4321 ctggcgtgat tatgttccgc aacctgcgcg taacggtttg agcaacttta ccggcaacct
     4381 tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc aggggatggt
     4441 ccactttacc cgctttttcc tgaataccat tttgggaatg ggcggcttta ttgatgttgc
     4501 agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta gtacgcttgg
     4561 tcattatggt gtgggttatg ggccgtacgt tcagttaccg ttctacggta gcttcacgct
     4621 gcgtgatgac ggtggtgata tggcggatag tctttacccg gttctttcct ggctgacctg
     4681 gccgatgtct gtgggtaaat ggacgcttga agggatagaa actcgtgcgc agttgttgga
     4741 ttccgacggt ctgctgcgtc agtcgtccga tccttatatt atggtgcgtg aagcgtactt
     4801 ccagcgtcat gatttcatcg ctaatggcgg tgaactcaaa ccgcaggaaa atccgaacgc
     4861 gcaagcgatt caggatgatt taaaagatat cgattctgaa taagaaacaa acaaaaaagg
     4921 tgagtcgcaa aactcacctt ttttcttatt aagctaacgc gttatcagaa cgcgtagtta
     4981 aagttagtac cgaacagcca ggctttacct tcagactcga actgatatgg gccttcgtta
     5041 attttcacgc tctgaccgtg catataagaa acaccaacgt cgactgaagc atctttatta
     5101 aatgcgtaag tcgtacctgc actcagccag aaacggtcct ggtccggaat ggagatagaa
     5161 cgattctgtg ccggaactgg gctgtcatca aaggcgatac cggtacggaa ggtccagtta
     5221 tcatcgtagt aataagtggt acccaacgcg atgcggtaag catctttaaa gccttcatgt
     5281 ttctggaaca gcgtgtcgcc actcgttgag gtcgctttca gctgctggaa ctgactccag
     5341 ctggtgtaag ccaggctata gtgaattgcc cactgcggag caacacggtt ataacccgac
     5401 acttcccaca tttcaggcag gttcagcgtc agataacctg actgcgttgc gccacctgtc
     5461 gcggtaggaa ttggcaaacc gtagttatta aatgcacgat taagatcgct gctgtagtta
     5521 cctttgaagt caattttcac ttcagaacgg taggtcagtg catagcggtt atttttatcc
     5581 agttcataca ggattccggc gttccagcca aaaccccact ggttaccgtt cagatgagcg
     5641 attttggtat tactgtcgat accgttggcg gtagctgcca atgcttgccc ttgctgagtt
     5701 tgtccagcag gagattgcat aatctgaccg gcaaccagct gccccagatc gcctgcgaaa
     5761 cgttcaattt tcgcgcgagc gtagacggcg ttgaaaccaa gaccaaagct ccatgcatta
     5821 tttaagcgat acgcaccgct taagttcagg ttcatggttt caaggtcggt tgtaccaccg
     5881 acagaaccgc ctgcataagt atcgttaaac tctgttgcca gaccatagtt agaggtaata
     5941 gaagcgcccc aaccaaattg gtcgttaatc ggtgcaacaa agtgcatgtt cggaacccat
     6001 gccgtaggcg cgatgttatc ggctttcagg ctacgaccag acggagacgt tccgctgata
     6061 tttacatccg gatcaatata aaccgcaccc gcagaaaatg tcgggcggtc aaacatggta
     6121 atcaatgcgg ggttacggct aacgttacct gcatcatcgg caattgcacc ttcccctgaa
     6181 taagcccggc ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc
     6241 tgggtggaga taagtgccac tgcgactgcg agagcagact ttgtaaacag ggttttctgg
     6301 ctcatgacca taacctcaat gatttatttt tatacaaact atgttacgtg ctgtaacagg
     6361 agcgcgaagt gtagggtctg aggtacatct tagaaatcag accagtggcg agagtatagg
     6421 tcggaccagc tggaatgttg caagtatgtt tctatctttt ttcagatatg atctaagaaa
     6481 cgcgatccag ctggcaaaat tgggaggtgg cgcactcatg gattcgggtg aattttgttt
     6541 tagatcattt ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt aacaactgga
     6601 gcagcacttt ccggaagcgt aaaatatcca caacgtttat ctggcacctt gggtgcacat
     6661 acagaggaaa tcaacgatga gtaaatgtag tgctgatgaa accccggttt gctgctgtat
     6721 ggatgttggc accattatgg acaactccga ctgcaccgcg tcttacagcc gtgtgtttgc
     6781 aaaccgcgca gaagcagaac aaacgctggc ggcgctgact gaaaaagccc gtagcgtgga
     6841 atccgaaccg tgcaaaatca ccccgacttt cactgaagag tctgacggtg tgcgcctgga
     6901 tatcgatttc acttttgcct gcgaagctga aatgctgatc ttccagctgg gtctgcgtta
     6961 attcctctcg ggtacatacg cccctgtcag ttgctggcag gggcgttttc ttttctcttc
     7021 ttcttttctc ttcttctttt ctctctgtgt cataactccc actttccctt ttctccactt
     7081 ggctaaatgt aaaaaaatgg ttaagactat gatcaggtca gactacttta tttatttttt
     7141 tacaggggag tgttatgggt caggttttac cgctggttac ccgccagggc gatcgtatcg
     7201 ccattgttag cggtttacgt acgccttttg cccgacaggc gacggctttt catggcattc
     7261 ccgcggttga tttagggaag atggtggtag gcgaactgct ggcacgcacc gagatccccg
     7321 ctgaagtgat tgaacaactg gtcttcggtc aggttgtgca aatgccggaa gcgcccaaca
     7381 ttgcccgtga aattgttctt ggcactggaa tgaatgtgca taccgatgct tacagcgtta
     7441 gccgcgcttg cgctaccagt ttccaggcgg ttgcaaacgt cgcagaaagc ctgatggcgg
     7501 gaactattcg agcggggatt gccggtgggg cagattcctc ttccgtattg ccaattggcg
     7561 tcagtaaaaa actggcgcgc gtactggttg atgtcaacaa agcccgcaca atgagccagc
     7621 gcctgaaact tttttctcgc ttacgtttgc gtgacttaat gccggtgcct ccggcagtgg
     7681 cggaatactc cactggatta cggatggggg acaccgcaga gcaaatggcg aaaacctacg
     7741 gcatcacccg agaacagcaa gatgcactgg cgcaccgttc gcatcagcgt gccgctcagg
     7801 cgtggtcaga aggtaaactc aaagaagagg tgatgactgc ctttatcccc ccttataaac
     7861 aaccgcttgt cgaagacaac aatattcgcg gtaattcctc tcttgctgat tacgcaaagc
     7921 tgcgtccggc gtttgatcgt aaacacggga cggtaacggc ggcgaacagt acgccgctga
     7981 ccgatggcgc ggcagcggtg atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg
     8041 taccgctggg gtatctgcgc agctacgcat ttactgcgat agatgtctgg caggacatgt
     8101 tgctcggtcc agcctggtca acaccgctgg cgctggaacg tgccggattg acgatgagcg
     8161 atctgacatt gatcgatatg cacgaagcct ttgccgccca gacgctggcc aatattcagt
     8221 tgcttggcag cgaacgtttt gctcgcgacg tactggggcg tgcacatgcc actggcgaag
     8281 tggacgatag caaatttaac gtgcttggcg gttcgattgc ttatggacat cccttcgcag
     8341 cgaccggcgc gcggatgatt acccagacat tgcatgaact tcgccgtcgt ggcggtggat
     8401 ttggtttagt gaccgcctgt gctgcgggtg ggcttggcgc agcaatggtt ctggaggcgg
     8461 aataatggaa atggcatcag cgtttaccct taatgttcgt ctggacaaca ttgctatcat
     8521 caccatcgac gtaccgggtg agaaaatgaa taccctgaag gtggaatttg cctcgcaggt
     8581 acgcgccatt attaagcaac tccgtgaaaa caaagagttg cgtggcgtgg tgtttgtctc
     8641 cgcgaaacca gacaacttca ttgctggcgc agacatcaag atgatcggca actgcaaaac
     8701 ggcgcaagaa gcggaagtac tggcgcggca ggggcaacag ttaatggcgg agattcatgc
     8761 tttgcccatt ccggttatcg cggctattca tggcgcttgc ctgggcggcg ggctggagtt
     8821 ggcgctggcg tgccacggtc gtgtttgtac tgacgatcct aaaacggtgc tcggtttgcc
     8881 tgaagtacaa cttggattgt tacccggttc aggcggtacc cagcgtttac cgcgcctgat
     8941 aggtgtcagc acagcattag agatgatcct caccggaaaa caacttcggg cgaaacaggc
     9001 ggtaaagctg gggttggtgg atgacgtcgt tccgcactcc atattgctgg aagccgctgt
     9061 tgagctggca aagcaggatc gcccatcttc acgccctcta cctgtacgcg agcgtattct
     9121 ggcggggccg ttaggtcgtg cgctgctgtt caaaatggtc ggtaagaaaa cagaacacaa
     9181 aacgcaaggt aactatccga cgacagaacg catcctggag gttgtcgaaa cgggattagc
     9241 gcagggcacc agcagcggct atgacgctga agcacgggcg tttggcgaac tggcgatgac
     9301 gccgcaatcg caggcgctgc gcaatatctt ttttgccagt acggaagtga aaaaagatcc
     9361 cggcagtgat gcgccgcctg cgccattaaa cagcgtgggg attctaggtg gtggcttgat
     9421 gggcggcggt attgcttatg tcactgcttg taaagcgggg cttccggtca gaattaaaga
     9481 tatcaacccg cagggcataa atcatgcgct gaagtacagt tgggatcagc ttgagggcaa
     9541 agttcgccgt cgtcatctca aagccagcga acgtgacaaa cagctggcat taatctccgg
     9601 aacgacggac tattgcggct ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa
     9661 tctcgaatta aaacaacaga tggtggcgga agtggagcaa aattgcgcta ctcataccat
     9721 ctttgcttcg aacacgtcat ctttaccgat tggcgatatc gccgctcacg ccgcgcgacc
     9781 tgagcaagtt attggcctgc atttcttcag tccggtggaa aaaatgccgc tggtggagat
     9841 tattcctcat gcgagtacat cggcgcaaac catcgccacc acggtaaaac tggcgaaaaa
     9901 acagggcaaa acgccaattg tcgtgcgtga caaagcgggg ttttacgtca atcgcatctt
     9961 agcgccttac atcaatgaag ctatccgcat gttgaccgaa ggcgagcgta ttgagcatat
    10021 cgatgccgcg ctggtgaaat ttggttttcc ggtaggccca atccaacttt tggatgaggt
    10081 aggaatcgac accgggacta aaattatgcc tgtactggaa gccgcttatg gagaacgttt
    10141 tagcgcgcct gcaaatgttg tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa
    10201 tggccggggt ttctatcttt atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc
    10261 cgccatttac ccgctcattg gcgcacaagg gcaggggcga ctctccgcac cgcaggttgc
    10321 tgaacggtgt gtgatgttga tgctgaatga agcagtacgt tgtctggacg agcaggttat
    10381 ccgtagcgtg cgtgacgggg atattggcgc ggtatttggc attggttttc cgccatttct
    10441 cggtggaccg ttccgctata tcgattctct cggcgcaggc gaagtggttg caataatgca
    10501 acgactggcc acgcagtatg gttcccgttt taccccttgc aatcgtttag ttgagatgag
    10561 cgagcgtggg gaaagttttt ggaaaacaac tgcaactgac ctgcaataag aaggtcaaag
    10621 ttatatgaat ccgcgctgaa tggcggagtg ttgctcaaaa tgtaaacgca tattgactat
    10681 acttacgcca ttgaggtaaa aaacagcgtt tcattcggtg aatggataag gcacaatgcc
    10741 ggccaccgtt ttctttctct ggtttcagat gaaagaaaac gggcgaatct ggttaacaaa
    10801 agcggtgcaa tatgcaagtt tttatcatgc gtcacggcga cgcagccctc gatgccgcca
    10861 gtgattcggt tcgtcctctg actactaacg gttgtgacga atctcgcctg atggcgaact
    10921 ggctgaaagg tcaaaaagtg gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg
    10981 agcaaacact ggaagaagta ggggattgtc tgaacctgcc ttccagtgca gaagttctgc
    11041 cggagttaac gccctgcggc gatgttggtc tggtcggcgc ctatttgcag gcgttgacca
    11101 atgaaggtgt tgcttcggta ctggtgatct cccacttacc gttagtcggg tatctggttg
    11161 ccgagttatg tccgggcgaa acgccgccta tgtttaccac ttcagcgatt gccagcgtaa
    11221 cgcttgatga aagcggaaaa ggccaattca actggcaaat gagcccttgt aatttgaaga
    11281 tggcaaaagc aatttaagca atcaatgccc ggttacgtgc gcgtacaccg ggcagtttct
    11341 ccgtcataca atcattcttt ttcttgtcta aatgttgtta
//
LOCUS       DAZFRG010000156        11355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFRG010000156 DAZFRG010000000
VERSION     DAZFRG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.156"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(66..1256)
                     /gene="bcr"
                     /locus_tag="ACHKUC_003327"
     CDS             complement(66..1256)
                     /gene="bcr"
                     /locus_tag="ACHKUC_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO6619533.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1284..1979)
                     /gene="rsuA"
                     /locus_tag="ACHKUC_003328"
     CDS             complement(1284..1979)
                     /gene="rsuA"
                     /locus_tag="ACHKUC_003328"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO6619534.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2129..3889
                     /gene="radD"
                     /locus_tag="ACHKUC_003329"
     CDS             2129..3889
                     /gene="radD"
                     /locus_tag="ACHKUC_003329"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6619535.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHEKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4014..4298
                     /gene="rplY"
                     /locus_tag="ACHKUC_003330"
     CDS             4014..4298
                     /gene="rplY"
                     /locus_tag="ACHKUC_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO6619536.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(4437..5444)
                     /gene="yejK"
                     /locus_tag="ACHKUC_003331"
     CDS             complement(4437..5444)
                     /gene="yejK"
                     /locus_tag="ACHKUC_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO6619537.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5626..5853
                     /gene="yejL"
                     /locus_tag="ACHKUC_003332"
     CDS             5626..5853
                     /gene="yejL"
                     /locus_tag="ACHKUC_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO6619538.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5873..7633
                     /gene="yejM"
                     /locus_tag="ACHKUC_003333"
     CDS             5873..7633
                     /gene="yejM"
                     /locus_tag="ACHKUC_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO6619539.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAVGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7708..7784
                     /locus_tag="ACHKUC_003334"
     tRNA            7708..7784
                     /locus_tag="ACHKUC_003334"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7742..7744,aa:Pro,seq:ggg)
     gene            complement(7910..8683)
                     /locus_tag="ACHKUC_003335"
                     /pseudo
     CDS             complement(7910..8683)
                     /locus_tag="ACHKUC_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            9003..9650
                     /gene="narP"
                     /locus_tag="ACHKUC_003336"
     CDS             9003..9650
                     /gene="narP"
                     /locus_tag="ACHKUC_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO6619540.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(9685..10737)
                     /gene="ccmH"
                     /locus_tag="ACHKUC_003337"
     CDS             complement(9685..10737)
                     /gene="ccmH"
                     /locus_tag="ACHKUC_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO6619541.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(10734..11291)
                     /gene="dsbE"
                     /locus_tag="ACHKUC_003338"
     CDS             complement(10734..11291)
                     /gene="dsbE"
                     /locus_tag="ACHKUC_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO6619542.1"
                     /translation="MKRKVLLIPLIIFLAIATALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT         2838 a         3045 c         2790 g         2682 t
ORIGIN      
        1 tgatagattt gtggttgaca tgtatcaaca aaggaagcct tttagcttcc tcgttgtgca
       61 atagatcacc gttttttcgg ccgactggcg tacaaacaga agagaatgga actggttgcg
      121 cagaatgcaa ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagtaat
      181 gcgccaacaa ttgcccctat gccgaaacgg aaggttcctg ccagcgaaga tgccgttccc
      241 gccatatggg gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg
      301 cagcccacaa acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgcg
      361 ctaatgacca tccacgctgc cataataaac tgtatccaca accccgagcg gaacatattt
      421 aacgcgccaa tacggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaga
      481 atgtttagcg caaagtaata accaaagttt tccggcgcga cgtggttgat ttcaatgtag
      541 acaaacggtc cggcgcttaa gaatgagaac atcccggcaa agctgaaacc gctggccagc
      601 atataactca ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga
      661 atgtgaaaag gctggcgacg ctccggtggt aaggtttctt tgatcagaaa gaagatcatt
      721 gccgaagcca gaatcgccgc cacggcgagg atccagaaga tgtaatgcca gctcagccac
      781 accagcaccc agccgccaac tatcggtgcc atcagcggtg caatggtcgt caccagcatg
      841 acaaacgaca tcatccgtga gaactcttct ttcgggtaga tatcacgcat caaagcgttg
      901 ataaccacgc tggccgcagc cgcagccagc ccgtggaaga aacgcatcac aatcagctga
      961 tcgatggttt gcgccaacgc acacgccacc gcggcggcgg caaacaccag cgtaccgcca
     1021 agaaccaccg gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac
     1081 gcaaagccca gaatataagt actcagggtc atctgcgtac tgcccgccgg tacaccaaac
     1141 tgcgctgaaa ttaccggtag cgcaggcaga tacatatcaa tcgacagcgg catcaacatg
     1201 gccagcaggc caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg
     1261 ggctcctgaa agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga
     1321 cgatattcac cgggggctaa atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc
     1381 agctcaacca catggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct
     1441 tcgctgatgg tcagacgaac ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg
     1501 agatcttttt cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct
     1561 acgggtgatt ccagcgtcac cagataggtc ttctcgcaat gatggcgcgg ggaagtaatg
     1621 cggtgcgacc actgaccatc atctgtcatt agtaccagac cggtggtatc gatatccaga
     1681 cgccctgcag cgtgcagttt ccacgctacc ggttcatcaa gaaaatagag caccgttggg
     1741 tgatccgggt cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt
     1801 ggaccgtgtt gctgcgccag cgggttgcca tcgtaagcga catcatgttc agggagcagt
     1861 ttgaacgctg cattacggac gatttcgcca tcgacggtga cacgattgcc gcggatttca
     1921 cgcccggcaa tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg
     1981 tgtgtgattt tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc tgaactgttg
     2041 tctgcccagt atagcggtct aataacttcc ctcaagggaa aaagatccgt ggcatactat
     2101 tagcagaata atctacctac gcgagaccat gatttttaca ctccgcccat atcagcaaga
     2161 agccgtggat gccacgctca accattttcg tcgtcataaa acccctgccg taatcgtgct
     2221 gcccaccggt gcaggtaaaa gcctggtgat agcggaactg gcgcggctgg cgcgtggtcg
     2281 cgtgctggtg ttggcacacg ttaaagaact ggtggcacaa aaccatgaaa aatatcaggc
     2341 gctggggctg gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa
     2401 agtggtattt ggcagcgtgc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt
     2461 ttcgctgttg attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca
     2521 gcaaatcctc actcacctga ccaaagtgaa tccccactta cgcctgctgg ggctgactgc
     2581 cacgcctttt cgactgggca aaggctggat ctaccagttc cattatcacg gcatggtacg
     2641 cggcgatgag aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat
     2701 taaacacggc tatctgacgc cgccagaacg gctggatatg ccagtagtgc aatacgattt
     2761 cagccgcttg caggcacaga gtaacggact gttcagcgaa gccgatctca accgcgagct
     2821 gaaaaaacag caacgcatta cgccacacat catcagccaa atcatggagt ttgctgcaac
     2881 gcgcaaaggg gtgatgattt tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt
     2941 actgcctgcc gaagatgcag cactgattac tggcgacaca cccggcgctg agcgcgatgt
     3001 attaattgat gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cggtactgac
     3061 caccggattt gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcggt
     3121 gagtctttac caacaaattg tcgggcgcgg cctgcgcctg gcccccggca agactgattg
     3181 cttaattctt gattatgcgg gtaaccctca cgatctctac gcgccggaag ttggtacacc
     3241 gaaaggcaaa agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa
     3301 caccttttgg gggaaaacga ctgccgacgg gacattgatt gaacactttg gtcgtcgctg
     3361 tcagggatgg tttgaagatg acgacggaca tcgcgaacag tgtgacttcc gtttccgttt
     3421 taaaaattgc ccgcaatgta acgcagaaaa tgatattgcc gcccgccgtt gccgcgagtg
     3481 tgataccatt ctggttgacc cggatgatat gttaaaagcg gcgctacgac tgaaagacgc
     3541 gctggtatta cgctgtagcg gcatgtcttt gcagcatggg cacgacgaaa aaggcgaatg
     3601 gttgaaaatc acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca
     3661 aacgcccgcc cagcgaactg ccttcgagca gctttttatc cgcccgcata cgcgcacacc
     3721 gggcatcccg ctgcgctgga tcaccgccgc cgatgtcctc gcccagcaag ccttattgcg
     3781 acacccggat tttgtcgtcg cccgcatgaa aggccagtac tggcaagtgc gtgaaaaagt
     3841 gttcgattac gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg
     3901 attgatgtat aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat
     3961 cacttacctg ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta
     4021 ctatcaacgc agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg
     4081 ctaacaagtt cccggcaatc atctacggtg gcaaagaagc gccgctggct gtcgaactgg
     4141 atcacgacaa agtcatgaac atgcaagtta aagctgaatt ctacagcgaa gttctgacca
     4201 tcgttgttga cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca
     4261 aaccgaagct gctgcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa
     4321 ccccgctccg gcggggtttt ttgtctctgc aaattatgcc tgattacggt atcgctattt
     4381 tttgcaggcc agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa
     4441 ttgccgccag acgtccggcg ctgcaattga tcgcgcaagt tcggcggcgt gcctttaatg
     4501 gtcagggtat cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag
     4561 ttaatagtca agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg
     4621 tctgccggga agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc
     4681 acttcgctga cgcctgccag ctctttcgac agcgattcca gttcaatctc ttcacctgcc
     4741 tgcagctgct cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta
     4801 tccagttgtg cttcggcagt gaaatcatcg accgcttgca acagcccacg gttttgcgct
     4861 ttcgcgttta acccttcgct ggcaccgagg aaatccataa agaaatccgc cactttgcgc
     4921 cctactcgcc ctttcaggaa agtgagatag cgggtcgact ctggattggt ttcccactcg
     4981 gttaaatcga tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg
     5041 atgtcgagat tttcgttaac acgcatactg ctcaggttgc tcagaactgc caccagcaga
     5101 tactccaccg ccagatagcg atagtggcaa aacagcacga atccaccgtc agcaaaagga
     5161 tatttcgcta attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc
     5221 tcttcgccct gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag
     5281 ccgtaagctt tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg
     5341 gtttccgtcg gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc
     5401 ttgataagct ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta
     5461 agaccgggcg gtattcaacc accgccggtt aagcgacgca ataaaagatg gcggtcattt
     5521 tacagaaggc agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg
     5581 ctacggtaat atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc
     5641 cgctacagtg atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa
     5701 cataaggctc cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc
     5761 aacaccagca ttgccccggc ccaacgccag gcaattgcca actcttttgc ccgcgcctta
     5821 cagtcctcta tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac
     5881 tcatcgtcag cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc
     5941 actgttcaat attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg
     6001 gccgacaacg cttgctggtc gcatttattc ctacgtaagc attatcggtc atttcagctt
     6061 cctggtgttc gccacctact tgctgatcct cttcccgcta acctttatcg tcggctccca
     6121 gaggctgatg aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc tattactgat
     6181 cgacagcgaa gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagctggt
     6241 tatcaaccca gacgaaaatg agatggcgcg cgactggcag ctaatgttca tcagtgtgcc
     6301 ggttatttta ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct
     6361 gacgcgtcgt cggcgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc
     6421 ctcgcatgtg gtttatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg
     6481 cgctaacctg ccgctttcgt acccgatgac ggcacgacgt tttcttgaga agcatggcct
     6541 gcttgatgcg caggagtatc aacgccgtct tattgagcaa ggcaatccag acgccgtttc
     6601 cgttcagtac ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct
     6661 gttgattact gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc
     6721 aggttttgct gagcaaaata tttcgttcac gcgtcatatg agctccggca acactacaga
     6781 caacggcatc tttggcctgt tttatggcat ctcgccaagc tatatggacg gcattttgtc
     6841 gacccgtacg cctgcggcgt taattactgc gcttaatcag caaggctatc agctggggtt
     6901 attctcgtca gatggcttta ccagcccgct gtatcggcag gcattgttgt cagatttctc
     6961 gatgccgagt gtacgcaccc aatccgacga acagaccgcc acgcagtgga tcaactggct
     7021 gggccgctac gcacaagaag ataaccgctg gttctcgtgg gtctctttca atggcacaaa
     7081 cattgacgac agcaatcagc aggcatttgc acggaaatat agccgagcag taggcaatgt
     7141 cgatgaccag atcaaccgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac
     7201 ggtggtgatt atcactgccg ggcggggtat tccgctgagc gaagaggaag aaacctttga
     7261 ctggtcccac ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca
     7321 gcgtattaat gcgctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct
     7381 acatgtcagc acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg
     7441 ccgccattac tgggttaccg ccgcggataa cgatacgctg gcaattacca ccccgaaaaa
     7501 gacgctggtg ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt
     7561 gaaagatgaa aaaccacagt taagtttgtt attgcaagta ctgacagacg agaagcgttt
     7621 tatcgctaac tgattaatta taaatcagtt agcaaaatat cttacttgca atcggtgtgg
     7681 aaaacggtag tattaacagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca
     7741 tggggtgtcg ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaac
     7801 caacctattg cggttggttt ttttatatct gcatttaatt cgataaacag acagcgacac
     7861 atcacgacct gtttattttc tgttatcaga acgtccagac tacacccgcc tgagttgtgc
     7921 tgctttcttc acgggagaaa cgactatcga taccgagcgt caggcccgtc aatgtttgct
     7981 caaagccagc tcccgcatca gtgttcacgt tgttgcagct gttaattgcc gaactccaca
     8041 tcgccgtatc gtaattaacg ccttttacgc taccaagacg cccacgcacg gtgtccagtt
     8101 ctgcatcaaa taccagcggt tgtgctgccg ccatattcag cacatccgtg gttgaagggg
     8161 gcatttgtac gcgattctct gcgagactcc agctatggtt gccattatcc agcaaggtat
     8221 attcatacgt actgatatca acaacgcttc cggcattgcc caacgtaaat gccgcatcac
     8281 cgcgccctcc tttaatcgtg ggatcagtcg ccgcgccata aacctgtagc actccattat
     8341 tgatcgtagt ctggtttacc gcgccaccca tttgaacaat ttgataacca tcatttaata
     8401 ctgagtattc tgcggtgcca tttatttcaa tgtactgata tcccccgtta atttcagtat
     8461 tactacttac acctaattct tatatatgct gttctccgcc aggattgatt ttcgtattat
     8521 tggcggtcca ataaaccagc tggatatctt tttccaggac aacaccatca actgtctcac
     8581 catactcaac agttaatgcc aatgctatgg gtgaatataa cgctgtagcc accagaacag
     8641 atattgcgga acgacaaaga gaaacagaac cagattggtg cattaagctt tcatcctatg
     8701 aaattaattg ctgttaaaag cattggggac agaaaatacc catagctcca tacccggagt
     8761 cagtttttaa aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt
     8821 ttcctgatga gaagctgatg caaaattccg tctttataat gaagatgatg ccaaagcgaa
     8881 cgacaaagtt gtagttttca ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc
     8941 tggtagcgtt accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta
     9001 ctatgcctga agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac
     9061 gcggtgttcg tcagttactg gagcttgatt ctggctttga agtggtcgcc gaagcgggcg
     9121 acggcgcaag cgcgatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc
     9181 tcaatatgaa aggcatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta
     9241 ccgcgcaaat tattatcctg accgtatcag atgcctccag cgatgtcttt gcactgatag
     9301 acgcaggcgc agacggttat ctgttgaaag acagcgaccc ggaagtgtta ctggaagcaa
     9361 ttcgtgccgg agcgaaaggc agcaaagtct ttagcgaacg cgtcaatcag tacttacgtg
     9421 aacgtgaaat gtttggcgcg gaagaagacc ccttcagcgt gctgacagaa cgcgagctgg
     9481 atgttctgca cgagctggca caggggctgt caaataaaca gattgcctcg gtgttgaata
     9541 tttccgagca gacagtaaaa gtccatattc gcaatctgct gcgtaaactc aacgtccgct
     9601 cacgcgtggc ggccaccatt ctgttcctgc aacaacgcgg ggcacaataa aaatagcccg
     9661 atggcatcac catcgggcca ctttttattt actctcctgc ggcgacaaat gttgcatcgc
     9721 ctgcgcgata ctacgttcga tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa
     9781 catcatctcc cacgcggcaa cggcttcgcc aaatcgttgc tgctcaaagg cattaaatgc
     9841 atacatgctt aacacacgga tattgctatg gtccgttctc accaactggc gcagcagttc
     9901 gccgccgagg cggttgtcgt tgggatcaga tgaacgtgtc aacgcttcag cgtatcccag
     9961 tgcagcatcg ctgtttttcg gatcgaggcg atacgcagtg gcgtatgcat cggtggcgat
    10021 actggcgtta cccagcgcca tgccaacgcg gcccaacata atccagcctt ctatatctcc
    10081 cggatttttt tgcagttgag tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt
    10141 gagcggatcg gctttcggat ccagcgccct gtccagtaac gccggagcct gtgccgtggc
    10201 ctgctgccag attttcacct gctgataatt gccagtctgg tagtagctga cgccagccac
    10261 aattaacgcc accacaatac ccggcagata aacaatatat ccggcacgct taccttccgg
    10321 cacgctttgt tctggaaacg cttccggcac cacgcgtacc cgacgccgcg aacgggcgta
    10381 tatgacccaa ccgccaatgc caatagccac tactggcagc acccacagca gcacggtcag
    10441 cggcgttaac ggcggatcgt aagtgacgaa gttgccgtaa cgcgccacca tataatcgac
    10501 aatctctttc ttacttttgc cttcctgcat cagttcatac actttctggc gcaggtcggt
    10561 ggcaatcatc gagttggaat cggcaatgct gttgttctgg catttcgggc agcgcagttc
    10621 ttcagtgagc tgacggaact gttgttcctg tgcttcatct ttaaactgca acacgtcgat
    10681 agtcgccagc gctgagccgg agatcatcag catcagcacg cccaataaaa acctcattgt
    10741 gcggcctcct tactgtattt ctcccacagt ggcttgatct cttcttccca gacgcgagga
    10801 ttgagatcgc ccgcatggcg atagcgaatg atgccgttgc cgtcaataag gaacgtttct
    10861 ggcgcgccat agacaccgag atccagccct aacatgccgt cgccatcaaa caggcttagc
    10921 gcgtaaggat tgcccagctc tttcagccag ctgattgcct tctggcgatc gtctttatag
    10981 ttcatgccga ccacgcggat gccctgcgca gaaagctgat tcagatattg atgttccgca
    11041 cggcaggtcg gacaccaggt cgcccagacg ttaagcagca ccggtttgcc ctgagtcagc
    11101 acatccgcct gataaaactg ccccggattg tccagtgatt cgaggcgaaa cttcggcact
    11161 ggcttgccaa tgagcgccga ttccagattg gtcggatcat ccccttcggc attacgcgcc
    11221 agctgccaca gtagcgccgt ggcaatcgcc aggaagataa tcaacggaat taacaatact
    11281 ttgcgcttca tacagcctcc ggcgcagttt tctgcgggtt cacgcgctta cgatagcgag
    11341 gatcaaacag gcaca
//
LOCUS       DAZFRG010000157        11326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFRG010000157 DAZFRG010000000
VERSION     DAZFRG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.157"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..692
                     /gene="gspC"
                     /locus_tag="ACHKUC_003339"
     CDS             <1..692
                     /gene="gspC"
                     /locus_tag="ACHKUC_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6619543.1"
                     /translation="VKNEQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQET
                     PKLSIALNGIVLTSNDETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFET
                     HGHYEKVTLHPGLPDIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFT
                     TSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELT
                     GMNGQRNEGTQ"
     gene            676..2628
                     /gene="gspD"
                     /locus_tag="ACHKUC_003340"
     CDS             676..2628
                     /gene="gspD"
                     /locus_tag="ACHKUC_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO6619544.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2638..4097
                     /locus_tag="ACHKUC_003341"
                     /pseudo
     CDS             2638..4097
                     /locus_tag="ACHKUC_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
     gene            4094..5290
                     /gene="gspF"
                     /locus_tag="ACHKUC_003342"
     CDS             4094..5290
                     /gene="gspF"
                     /locus_tag="ACHKUC_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6619545.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5300..5737
                     /gene="gspG"
                     /locus_tag="ACHKUC_003343"
     CDS             5300..5737
                     /gene="gspG"
                     /locus_tag="ACHKUC_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6619546.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5745..6254
                     /gene="gspH"
                     /locus_tag="ACHKUC_003344"
     CDS             5745..6254
                     /gene="gspH"
                     /locus_tag="ACHKUC_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6619547.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVIVADGQITPFSLLMANAGTGKPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6251..6628
                     /gene="gspI"
                     /locus_tag="ACHKUC_003345"
     CDS             6251..6628
                     /gene="gspI"
                     /locus_tag="ACHKUC_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6619548.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6621..7208
                     /gene="gspJ"
                     /locus_tag="ACHKUC_003346"
     CDS             6621..7208
                     /gene="gspJ"
                     /locus_tag="ACHKUC_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6619549.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7201..8184
                     /gene="gspK"
                     /locus_tag="ACHKUC_003347"
     CDS             7201..8184
                     /gene="gspK"
                     /locus_tag="ACHKUC_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO6619550.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPVQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLNTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8199..9362
                     /gene="gspL"
                     /locus_tag="ACHKUC_003348"
     CDS             8199..9362
                     /gene="gspL"
                     /locus_tag="ACHKUC_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6619551.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKFSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLSILSFVGSRGIAFWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHHELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9359..9820
                     /gene="gspM"
                     /locus_tag="ACHKUC_003349"
     CDS             9359..9820
                     /gene="gspM"
                     /locus_tag="ACHKUC_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO6619552.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9820..10497
                     /gene="gspO"
                     /locus_tag="ACHKUC_003350"
     CDS             9820..10497
                     /gene="gspO"
                     /locus_tag="ACHKUC_003350"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO6619553.1"
                     /translation="MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10526..11002)
                     /gene="bfr"
                     /locus_tag="ACHKUC_003351"
     CDS             complement(10526..11002)
                     /gene="bfr"
                     /locus_tag="ACHKUC_003351"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO6619554.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11074..11268)
                     /gene="bfd"
                     /locus_tag="ACHKUC_003352"
     CDS             complement(11074..11268)
                     /gene="bfd"
                     /locus_tag="ACHKUC_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO6619555.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         2974 a         2892 c         2690 g         2770 t
ORIGIN      
        1 tagttaaaaa tgaacagatc attaatcaac caacaaatgc atttcagtct gaattcagcc
       61 ttgccgcttt atggcgcaac gaaaatcatg ctggggtaaa agatgcaaat cccgtggcag
      121 ttaatcagga aacacctaag ctctctatag cgcttaatgg cattgtactc acatcgaatg
      181 atgagacttc ttttgtctta attaatgagg ggaacgaaca aaagcgatac tccctcaacg
      241 aagcccttga gtcagcgccg ggaacgttta ttagaaaaat aaataaaacc agcgttgtgt
      301 ttgaaactca cgggcattac gaaaaagtaa ctctccatcc agggctacct gacatcatca
      361 agcagcctga ttcggaaaat caaaatgtac tcgccgatta tattatcgcc acacctatac
      421 gtgatggaga acagatatac ggtcttcgat tgaatcctcg taagggtctc aatgcattta
      481 ccaccagctt gctgcaacct ggggatatcg ccctcaggat caataatctt tcgctgaccc
      541 accctgatga ggtttcacag gcattaagtt tactgctaac tcaacaaagt gctcagttta
      601 caattcgtcg caacggcgta ccccgcttga taaatgtttc cgtcgcggaa cttacaggaa
      661 tgaatggaca gcgcaatgaa gggactcaat aaaatcacct gctgcttgct ggcagcacta
      721 ctcatgcctt gtgcaggaca cgctgagaac gaacaatacg gcgctaactt caataacgcc
      781 gatatccgcc agttcgtgga aatagtgggt cagcatcttg gcaaaacgat cctgatcgac
      841 ccttcggtac agggaaccat ttccgtacgc agtaatgata cgtttagcca acaggagtac
      901 taccagttct ttttaagtat tcttgatctt tacggttatt ccgtgatcac actggacaat
      961 ggttttctga aagtggtgcg ctcagctaat gtaaaaacat cgccagggat gattgctgac
     1021 agttctcgtc caggcgtagg tgatgagttg gtcacccgaa tcgtaccgct tgagaacgtt
     1081 cctgctcgtg acctggctcc cctgctccgc cagatgatgg atgcgggtag cgtcggtaat
     1141 gttgtgcatt atgaaccctc caacgttctt attctgaccg gtcgtgcctc caccattaat
     1201 aaactgattg aagtcataaa gcgcgttgat gtcatcggca cagagaagca gcaaattatt
     1261 catctggaat atgcatcagc ggaagatctc gccgagattc ttaatcaatt aatcagcgaa
     1321 agccacggta aaagccagat gcctgccctc ctttccgcga agattgtggc ggataagcga
     1381 accaactctc ttatcatcag cgggccggaa aaagcacgcc agcgcataac ttcattactg
     1441 aaaagccttg atgtagaaga gagcgaggaa ggaaataccc gggtctatta cctgaaatat
     1501 gctaaagcca cgaatctggt ggaagtgcta accggtgttt ccgaaaagct gaaagatgaa
     1561 aaagggaatt cgcgtaagcc ttcttctact tctgcgatgg ataatgtggc cattaccgcc
     1621 gatgaacaga ccaactcgtt ggtcattacc gctgaccagt ccgtccagga gaaactcgcc
     1681 acggtaattg cgcgtctgga tattcgccgt gcacaggtgc tggttgaggc aatcatcgtc
     1741 gaagttcagg atggaaatgg actcaacctc ggcgtgcaat gggcgaataa aaacgttggc
     1801 gcacagcaat ttaccaatac gggattaccg gtttttaacg ctgcgcaagg tgtagctgat
     1861 tataaaaaga atggcgggat caccagcgcg aatcctgcct gggatatgtt tagcgcctac
     1921 aatggcatgg ctgcaggctt cttcaatggc gactggggcg tattgttgac tgcgctggcc
     1981 agtaacaata aaaatgacat cctcgccacc ccgagcatcg taacgctgga taataaactc
     2041 gcgtccttca acgttggtca ggatgtgccg gtgctatccg gctctcagac cacttctggg
     2101 gataacgtct ttaatactgt cgaacgcaaa acggtgggca caaaactcaa agtcactccg
     2161 caggtcaatg aaggcgacgc ggtactgctc gaaatagagc aggaggtttc cagcgttgac
     2221 tcttcgtcta attcgacgct cggcccgaca tttaacaccc gtactattca aaacgctgtg
     2281 ctggttaaaa ccggtgaaac ggtggtcctg ggcggattgc tggatgattt ttctaaagag
     2341 caagtgtcaa aggttcctct gcttggcgat attcctttag tgggacaact cttccgctat
     2401 acctcgaccg agcgcgctaa acgcaacctg atggtattta tccgcccgac gattatccgt
     2461 gacgatgatg tttatcgctc actgtcaaaa gagaaataca cccgctaccg tcaggagcaa
     2521 cagctgcgaa tcgacgggaa atcaaaagcg ttggttggct cggaagattt gccggtgctg
     2581 aatgagaaca cgttcaacag tcacgcccct gcgccatcgt cacggtgagg cattcttatg
     2641 agaattcact caccgtaccc cgccagttgg gcgctggcac aacgaattgg ttatctctat
     2701 tcagagggcg agattattta tctcgccgat acgccattcg aacggttact cgatattcaa
     2761 cgtcaagttg gccagtgcca gaccatgacc agcttgtcac aggctgattt tgaagcccga
     2821 ctggaagcgg tgttccatca gaataccggt gagtcgcaac agattgcgca ggatatcgat
     2881 caatccgtcg atcttctctc gctttcggaa gagatgcccg caaatgaaga tctcctgaat
     2941 gaagattcag cggcaccggt tatccgcttg atcaatgcta ttttgagtga ggccatcaaa
     3001 gaaaccgcct ccgatatcca cattgaaacc tatgaaaaaa caatgtcgat ccgttttcgc
     3061 atcgacggcg ttttgcggac aattttacag cccaacaaaa aactggcggc actgcttatc
     3121 tcccgaatta aggtcatggc tcgtcttgat atcgccgaaa aacgtattcc acaggatgga
     3181 agaatcagtt tgcgtatcgg gcgacgtaac atagatgtcc gcgtatccac actgccatcc
     3241 atctatggtg aacgcgctgt gctccgcctg ctggataaaa acagcctcca gctttcattg
     3301 aacaacctgg ggatgacggc agcggataaa caggatttag aaaatctcat tcagcttccg
     3361 cacggtatta tcctggtaac agggccgaca ggctccggta aatttcggcg cttaataccc
     3421 ctggccgtaa tattctgacg gtagaagatc ccgtggaata tgaactggaa ggcataggac
     3481 aaacgcaggt gaatacccgc gtggatatgt ctttcgctcg cggcctgcgc gccatacttc
     3541 gccaggaccc ggatgtcgtc atggtggggg aaattcgtga tacagaaacc gcgcaaattg
     3601 cggttcaggc ctcgctcaca ggccatctgg tactctcaac actccacact aacagtgcat
     3661 caggcgcagt cacccggcta cgcgacatgg gcgtcgaatc attcctgctt tcgtcttcac
     3721 tggcagggat tatcgcgcaa cgtctggttc gtcgtctgtg tccgcaatgc cgacaattca
     3781 cgcccgtatc gccgcaacaa gcgcagatgt ttaaatatca tcagctcgcg gtgacaacaa
     3841 ttggcactcc cgtaggttgc cctcattgcc atcaatccgg ttatcagggg cgcatggcga
     3901 tccacgaaat gatggtggtg acgccggaat tgcgggccgc tattcatgaa aatgtggatg
     3961 aacaagcact ggagcgactg gtccggcaac aacacaatgc cttaatcaaa aatggcctgc
     4021 aaaaagtgat acgcggtgac acctcctggg atgaggttat gcgcgtcgcc agtgccacgc
     4081 tggagaacga agcatgaatt atcgctatcg cgccatgacc caggatggtc aaaaattgca
     4141 ggggatcatt gatgctaacg atgaacgtca ggcacgactg cggctgcgtg aagaagggct
     4201 tttcctgttg gatattcgcc cccaaaaaag ttcgggagta aaaacacgtc gcccgaggat
     4261 cagccatagt gaactaacgc ttttcacccg gcagttggca accttaagcg cagcggcatt
     4321 acccctggaa gagagccttg ccgtaattgg tcaacaaagc agtaataatc gactggctga
     4381 cgtgttaaat caggtacgca gcgccatcct tgaagggcat cccctttccg atgcgttaca
     4441 acattttccc acgcttttcg attcgctcta ccgtaccctg gtaaaagcgg gcgaaaagag
     4501 cggtctgctg gccccggtgt tggaaaagct ggctgattac aatgaaaacc ggcagaaaat
     4561 ccgcagcaag ctcattcagt cactgatcta cccctgcatg ctcactacgg tggcgattgt
     4621 ggtcgtgatt attctcctca ctgctgtcgt gcccaaaatt accgaacagt tcgtgcatat
     4681 gaagcagcaa ctgccactga gtacacgcat tcttttaggt ctgagcgaca cgttgcaacg
     4741 taccggcccg acattattag cgacagtatt tattgtcgct gtgggtttct ggctctggtt
     4801 aaaacgcggc aataaccgcc accgttttca tgccatgtta ctgcgcgttg cgctcatcgg
     4861 cccgctgatt tgcgccatta acagcgcacg ctatctccgc actttaagta ttttgcaatc
     4921 cagcggcgtc cctctgctgg atgggatgaa tttgtccacc gaaagcctca acaacctcga
     4981 aattcgccag cgcctggcaa atgcggcaga aaacgttcgc cagggtaaca gcattcatct
     5041 ttcactggaa caaaccgcaa ttttcccgcc gatgatgctc tacatggtgg cctctggcga
     5101 aaaaagcggg caactcggca cgttaatggt cagagccgca gataaccagg agacactcca
     5161 acaaaatcgg atcgccttaa cgctctccat cttcgagcca gcactcatta ttacgatggc
     5221 actgatcgtc ctgtttattg tcgtgtcggt actccaacct cttcttcaac ttaactcaat
     5281 gattaattaa ggaaatcata tgcgcgcaac ggataagcaa cgcggtttta cattactgga
     5341 aattatggtg gtaattgtca tcattggcgt actcgccagc ctggtggttc ccaacctgat
     5401 gggcaataaa gaaaaggcgg ataaacaaaa agccgtcagc gatattgtgg cgctggaaaa
     5461 cgccctggat atgtacaagc tcgacaatca tcgctatcca accacaaatc aggggcttga
     5521 atccttagta gaagcgccga cactgccacc gctggccgca aactataaca aggaaggtta
     5581 tatcaagcgt ctgcctgccg atccctgggg caatgattat gtcctcgtta atcctggtga
     5641 gcatggtgca tacgatctgc tttcagcagg acccgatggt gaaatgggaa ccgaggacga
     5701 catcaccaac tggggtttga gcaagaagaa aaagtaagtc agcgatgaat cagcaacgcg
     5761 ggtttaccct gctggagatg atgctggtgc tggcgctggt cgctatcacg gcaagtgtgg
     5821 tgctcttcac atatggtcgg gaggatgccg ccagcactcg tgcgcgcgaa acagcggcgc
     5881 ggtttaccgc tgcacttgaa ttagccatcg accgcgccac gctcagtggt cagcctgtcg
     5941 gtatccattt ctctgattca gcatggcgca ttatggtacc gggcaaaaca ccatcagcct
     6001 ggcgctgggt tccattacaa gaagatgccg cagacgagag caaaaatgac tggggtgaag
     6061 aactctcaat tcaacttcag ccatttaaac ctgatgacag caaccaaccg caggtggtga
     6121 ttgtggccga cggacaaatc acgcccttct ctttgttgat ggctaatgcc gggaccggta
     6181 agccgctcct gacgttagtc agttcaggct catggcccct ggatcaaacg ctcgccagag
     6241 atacccgccc atgaacaaac aatcagggat gacactgctt gaagtcttac tggcgatgag
     6301 tattttcacc gctgttgcgc tgactttaat gagcagtatg caagggcaac gaactgccat
     6361 cgagcggatg cgtaatgaga cgctggcatt gtggatagcg gataaccagc ttcagtcaca
     6421 agattcattc gacgaggaaa acacgtccag ttctggcaaa gagttaataa atggcgaaga
     6481 gtggaactgg cgcagcgata tccactcaag caaagacggc acgcttctgg aacacaccat
     6541 aacggtgaca ttacccagcg gtcagacaac ctcgcttacg cgctatcaaa gcatcaataa
     6601 taaatccggg caggcacaag atgattaatc gccagcaggg tttcacattg ctggaagtca
     6661 tggcggcgct ggcgattttc tcaatgctta gcgtgctggc gtttatgatt ttttctcaag
     6721 cttccgagtt gcatcaacgt tcacagaaag aaatccagca gttcaatcag ctacagcgca
     6781 ccatcaccat tcttgataac gatctcctgc aactggtcgc ccggcggaat cggagtacgg
     6841 acaaaatcat ggtgttgggt gaagaagcga tttttaccac gcaaagtcgc gatccgctgg
     6901 cccctctcag tgaagcacaa accttactca ctgttcactg gtatctgcga aatcatacgc
     6961 tctaccgtgc tgttcgtact tctgtggatg gcaggaagga tcaacccgcc caggcgatgc
     7021 tggaacatgt cgagagcttt cttctggaaa gtaacagcgg agaaagccag gagcttcctc
     7081 tctcagtcac gttacatctg aagactcagc aatatggggc gttacagcgc cgttttgcat
     7141 tacctgagca gttagcgaga gaagagtcgc cagctcagac gcaggccggg aataataacc
     7201 atgaataatg aacaacgcgg cgtagcactg ctcattgtac tcatgttact ggcgctcatg
     7261 gctgctctgg ctgcagacat gacgatcagt tttcacggcc agcttcatcg aacccgccag
     7321 gtaaaccatc atttgcaacg gcaatatgat attgagctgg cggagaaact tgcgcttgcc
     7381 agcctcaccc aggacgttaa agataacgac agacaaacca cgttgcagca gtactgggca
     7441 caaccgcaac agttgcagct ggaaaatggc aatacagtga agtggcagtt acgcgatgcc
     7501 cagcactgtt ttaatctcaa tgccttagca aaaatctctg acgccccgct ggcttcccct
     7561 gatttccccg tccaggtatt cagtgccctg ctgattaacg ccggtatcga tcgcggaaac
     7621 accgatgaga tcgtgcaatc aatcgctgac tatattgatg ccgatgattc gccacgcttt
     7681 cacggcgcag aagataactt ttaccaaagc caaacgccgc ctcgccatag cgccaatcaa
     7741 atgctttttc tgaccggtga attgcgtcag ataaaaggca taaccgaaaa catctatcag
     7801 cggcttatcc cgtatgtctg cgttctcccg acctcggaac ttagcatcaa tcttaatatg
     7861 ctaacggaga acgatattcc gctcttcaga gcattattcc tcaataacat cacggatgct
     7921 gacgctcggg tcttattaca aaaaaggccc agggaaggct ggctcaccac agatgcattt
     7981 ctttactggg cgcagcagga tttctccggc gtgaagcccc tggttgctca ggtgaaaggg
     8041 catctctttc cctatagccg ctattttacg ctgaatacgg aaagtatcag cgacgaacaa
     8101 tcccagggat ggcaaagcca tatttttttc aaccgtaaac agcagagcgc ccaaatctat
     8161 cggcgtacgc tgcaactcta ctaatcataa ggatcgtgat gcctgaatcg ttgatggtaa
     8221 tacgttcttt ctcgacgctg cgtaagcatt gggaatggat gacgttctcc gcagatagcg
     8281 tatcttcggt acatacgctc acagacgatc ttccgctgga gtcgctggcc gatcagcccg
     8341 gtgccggaaa tgttcacctt ttgatcccac ctgagggtct tttatatcgt tccctcacgc
     8401 tgcccaacgc caaatacaaa ctcaccgcgc aaacattaca gtggttggcg gaggagacat
     8461 tacccgacgc cagccaaaat tggcactgga cggttgtcga taaacaaaac gaaagtgtcg
     8521 aggtgatagg cattcagtcg gagaaattta gtcgttatct tgagcgttta catactgccg
     8581 gactcaacgt gacgcgggtt ttgccagatg gctgctatct tccctgggaa gtagatagct
     8641 ggactctggt taaccagcaa acaagctggc ttattcgcag tgccgcacat gccttcaacg
     8701 aactggatga acactggtta cagcatcttg ctaaccagtt tccgccagag aatatgcgtt
     8761 gttatggtgt agcgcctcac ggcgttgcag cggccaatcc acttatccag catcctgaaa
     8821 taccatcact gagtttgtat tctgccgata ttgcattcca gcgctatgac atgctgcatg
     8881 gtgtttttcg taaacagaaa actgtaagca aatcgggaaa atggctcgcc cgcctggcag
     8941 taagctgtct cgttctctcc attctttctt ttgttggcag ccggggcatc gcgttctggc
     9001 aaacgcttaa gattgaagat caacttcagc aacaacagca agaaacctgg caacgttatt
     9061 tcccgcagat caaacacacc cacaactttc gtttttactt taagcagcaa ctcgctcagc
     9121 aatatcctga agctgtaccg ttgctctatc atttacaaac acttctgctt gaacaccatg
     9181 aactgcagtt aatggaggcg aactatagcc agaggcaaaa gtcgctgacc ttaaaaatga
     9241 gtgctaagag tgaagccaat atcgatcgtt tctgtgagtt aacacagtcc tggcttccga
     9301 tggaaaaaac agaaaaagat ccggtcagtg gtgtatggac agtaaggaac tcagggaaat
     9361 gattaaatca tggtgggcag aaaaatccac atcggaaaaa cagatcgttg cggcattggc
     9421 ggttctctcg ctcggcgttt tttgttggct tgcggtgata aaaccgatag atacctacat
     9481 tgaagagcat cagtcccacg cgcaaaaaat taagaaggac atcaaatgga tgcaggatca
     9541 agcgagcacc catggtttac tcggtcatcc agcgcttacg cagcccatca aaaatatcct
     9601 tctcgaagaa gcaaaacgtg agaatttgac tatcacgctg gaaaacggtc cggacaatac
     9661 tctgacaatt aaccctgtca cagctcccct cgaaaacgtc tcccgctggc tcactacggc
     9721 acagataaca tacggtattg ttattgaaga ccttcagttt actcttgccg gaaacgaaga
     9781 gatcaccctc aagcatttgt ccttcaggga gcaacaataa tgacaatgct actgccgctc
     9841 ttcattctgg ttggttttat tgcaggttat tttgttaatg tcatggccta tcacctctcg
     9901 cctttggagg ataaaacggc gttaacgttt cgccaggtat tggttcattt ctggcaaaaa
     9961 aaatatgcct ggcatgatac agtgcccctg atactttgcg ttgctgctgc tatcgcctgc
    10021 gccctggcac ctttcacgcc cattgtgact ggcgcactct ttctctattt ctgtttcgca
    10081 ctcacgctca gtgttattga ttttcgcact cagctcctgc ccgacaaact caccttaccg
    10141 ctgctctggc ttggcttggt atttaatgcg cagtctggat taattgattt acatgatgcg
    10201 gtttatggcg cggtcgctgg ctatggggtg ctgtggtgtg tttactgggg cgtctggtta
    10261 gtttgtcaca aagagggatt gggctacggt gatttcaagc tactggccgc tgcaggcgcg
    10321 tggtgtggct ggcaaacgtt gccaatgata ctgctgattg cctcgctggg tggcattggt
    10381 tacgccatcg tttcacaact tctgcaacgc cggactataa ccactatcgc attcggcccg
    10441 tggcttgcgc tcggcagcat gataaacctg ggatatctgg cctggatctc ttattaaccg
    10501 ggagggttct ccctcccgac acggctcaac cttcttcgcg aatctgtgct tgcagataat
    10561 tttgcagtcc catcttctga atcagatcaa gttccgtttc cagccagtcg atatggcctt
    10621 cttcatcgcg caaaatttct atcatcatat cgcggctgac gtaatcatga acgctatcgg
    10681 cataaccaat tgcctcacgc aaattcttcg cgccatccag ctcaagtgcc agatcagaac
    10741 gcagcatttc ctcaacatct tcaccaatgt tcagtttgcc caggtcctgt aagtttggaa
    10801 gaccttccag aaaaagaatg cgctcaatat aacgatcggc gtgtttcatc tcatcaatgg
    10861 attcatgata ctccacatca ttgagacgtt tgagacccca gtttttaaac atccgggcat
    10921 gaagaaagta ctgattgatt gcgacaagct catttcccaa cagtttgttg agataattta
    10981 taactttagt atcacctttc attttttgac tccttcgctt ccactctttg aagcgtagaa
    11041 cggcttacga gtaagtcaaa aaacacactc cgtttatgcg gactccttaa actccggcag
    11101 ttgcattaat tcatcctcca tcacttcacg cgcggcccgg acacatttac cgcactgatt
    11161 gccgaccgga atgaattttt ttaattgctg gaacgagtgg ggggaaaact ggcgcacagc
    11221 ctgacgaatt tttttatcgc tgataccatt acaaagacaa acgtacatag agactcccgt
    11281 atactttctt caaaagtaaa tgaaaatagt tcttatttca attaca
//
LOCUS       DAZFRG010000158        11269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFRG010000158 DAZFRG010000000
VERSION     DAZFRG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.158"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..499
                     /locus_tag="ACHKUC_003353"
     CDS             <1..499
                     /locus_tag="ACHKUC_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO6619556.1"
                     /translation="DINKINTAIAGVIYLFLISLVAIVVASLAPVSAIIVVFSTAGSM
                     FLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERPIWVISCLMIVLWSG
                     IISHGRNKLLELTGKSHSEELWSSVRCAFIGALTLYYYFIGFFGILAAIAITLVWQRH
                     TRFFH"
     gene            complement(535..1491)
                     /gene="ybhN"
                     /locus_tag="ACHKUC_003354"
     CDS             complement(535..1491)
                     /gene="ybhN"
                     /locus_tag="ACHKUC_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO6619557.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1491..2732)
                     /gene="clsB"
                     /locus_tag="ACHKUC_003355"
     CDS             complement(1491..2732)
                     /gene="clsB"
                     /locus_tag="ACHKUC_003355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO6619558.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2729..3490)
                     /gene="ybhP"
                     /locus_tag="ACHKUC_003356"
     CDS             complement(2729..3490)
                     /gene="ybhP"
                     /locus_tag="ACHKUC_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6619559.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYLEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3623..4033
                     /gene="ybhQ"
                     /locus_tag="ACHKUC_003357"
     CDS             3623..4033
                     /gene="ybhQ"
                     /locus_tag="ACHKUC_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO6619560.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3995..5101)
                     /gene="ybhR"
                     /locus_tag="ACHKUC_003358"
     CDS             complement(3995..5101)
                     /gene="ybhR"
                     /locus_tag="ACHKUC_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6619561.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5112..6245)
                     /gene="ybhS"
                     /locus_tag="ACHKUC_003359"
     CDS             complement(5112..6245)
                     /gene="ybhS"
                     /locus_tag="ACHKUC_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6619562.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6238..7974)
                     /gene="ybhF"
                     /locus_tag="ACHKUC_003360"
     CDS             complement(6238..7974)
                     /gene="ybhF"
                     /locus_tag="ACHKUC_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6619563.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     NSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            complement(7967..8962)
                     /gene="hlyD"
                     /locus_tag="ACHKUC_003361"
     CDS             complement(7967..8962)
                     /gene="hlyD"
                     /locus_tag="ACHKUC_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO6619564.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8965..9636)
                     /gene="cecR"
                     /locus_tag="ACHKUC_003362"
     CDS             complement(8965..9636)
                     /gene="cecR"
                     /locus_tag="ACHKUC_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO6619565.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9865..11226
                     /gene="rhlE"
                     /locus_tag="ACHKUC_003363"
     CDS             9865..11226
                     /gene="rhlE"
                     /locus_tag="ACHKUC_003363"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO6619566.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRTESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
BASE COUNT         2610 a         3092 c         2996 g         2571 t
ORIGIN      
        1 agatatcaac aagataaata ccgccatcgc gggcgtaata tatttgtttt taatctcttt
       61 ggtggcgatt gtcgttgcga gtttagcgcc tgtatctgcc attatcgtcg tgttcagcac
      121 tgcgggatcg atgtttttaa tcagtatgct ggcaggtcta ttgtttaaat ttgatcctgg
      181 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
      241 tgccacatta atgagtgaac gacccatttg ggtaataagt tgcttaatga ttgtcttatg
      301 gtcaggcatt atctcgcatg gacgaaataa gctccttgaa ctgacaggaa aaagccatag
      361 tgaagaatta tggagttcgg ttcgttgtgc ttttataggt gcattaacac tctattacta
      421 ttttatcggc ttctttggga ttcttgccgc tatagctata acgcttgtct ggcaaaggca
      481 tacgcggttt tttcattagc cgtaaatgca catactaaag aacttaacta tacttcacat
      541 cgccgcttca tttttcgccc gtagcttctt cgcctggctt tccagcaaca ggtaacagat
      601 cagcgccagc agcagtggga taaagtaata cagcacacgg taagcgagca gggcggcgat
      661 aattgtgcct ttggaggtat gctccccagc cagtagcgcg ataaacaccg cttccagcac
      721 accaattccc gccggaatat gcacgatgac gccagcaata ctactaacca gcaacacgcc
      781 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg ctcccattac
      841 catccagtta acgctggaaa tcagcatctg ggtgagggcg aatttccacg aaggcagcac
      901 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
      961 aaccgcgata atcatcagta agccaatacc gagaatacgc agcgtagttt gatcgacata
     1021 ccagtgatcc ggcaactcca ccacgcctgc ggtaaagata acccccgcta gcaaaatata
     1081 gcctagccag ttggtggtaa tactgagcga aaaaatccgc gtaatagtgc tgcccggtaa
     1141 ccccagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagtgt
     1201 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
     1261 accgcagtag aaacgggcga gcaggtcgta gcaaccgtaa atcagataac tgacgaccac
     1321 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
     1381 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
     1441 ccaggtgagg agtttctttg ctaagcgcca gcgcgggtgt gatttactca tcagggtttt
     1501 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
     1561 ccaacctgcg ccagacgtgg cgtgtgcgcc ggaagccagc ccaccagcgc cgggaagtgg
     1621 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttcggcagc
     1681 atggtctcat cgacctgctg acaatctgcg gcgataatgc cgttcagatt atcgcgcagc
     1741 gtctggttaa aattacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
     1801 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
     1861 ccgtggagcg ggcggcggcg gtactcaaat acctgaacgc cgcctttaac cagatagtta
     1921 tacagcaaac gtgcgccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
     1981 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaaacg atagccgggg
     2041 aagaagtagg cgttggcgat aatcacttcc cgccgcgcct gagtgagcat tttcagatag
     2101 tgacgttcga tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
     2161 tctcccggct ggcggttctc ttccgcttta tgatgacgtc gccaccagcg tcgcgccgcg
     2221 ctctgtcctg gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttcg
     2281 aggcgaaccg cgtaatcctg tttggcctcc gggccgtagc tggacatatg ctcggcggag
     2341 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
     2401 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
     2461 accacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
     2521 ccatccagca agacttccgc ttttaccccg cgttgcgctg ctgccaatag cgccgcatgc
     2581 agctgtttgc cgacgtcatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgc
     2641 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
     2701 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
     2761 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
     2821 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
     2881 ggcgtccgtg ggcgcgggta aaaatctcat ccagcccggc ctgcactttt aacggatgat
     2941 tagctttttg ccgccagtcg ttgaaatcac ccgctaccaa taccggttcg ccgtccggta
     3001 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
     3061 ccagatgtac gcacatcaca tgaatcgctt tcccggtcat cggcggcaca atgcggcagt
     3121 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc acgattctca taatgttcaa
     3181 tgggataacg tgaaagaacg gcgttaccgt gatgtccttc caggtatacg gcattgcgac
     3241 cgtaggcgaa atcgctccac atggtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
     3301 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
     3361 tatcggcgct gacggtacgc acggcgtcgc gaagttccgg caaaatgaag cgtcggttaa
     3421 acgcggtaaa gcctttgtga atattgatgg tgagcacctt gaacgaaaat tgttgtgttt
     3481 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
     3541 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgaa taagtagaga
     3601 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caactggcct
     3661 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
     3721 gagtaaaact ctggttcacg tcggcgtagg attatgcgca ctgtattgtg tcacggtagt
     3781 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
     3841 ggaagagctg accacgacct ggtattttgg tgcagcgctg attgtgctgt ggctgttgtc
     3901 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcaatcc ttgccggccc
     3961 tgcggtggtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
     4021 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
     4081 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
     4141 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
     4201 aaccggaaag gagaatggcg ggcatcataa agacaaatac gccgataaat gcctgctgtt
     4261 gcgttgaaca gagcgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
     4321 ccatcgtaaa gtagaacagc gccagcgatc cggcgaaggg gatttgatac gcccagatac
     4381 caatcgccag cacaatggtg gcctggaagg tggcgacaat taatgccggt acggctttgc
     4441 cgatgaatat ctgccaggtg gtgaggggcg aaaccagcaa ctgatcgagc gtaccttgtt
     4501 cacgttcacg ggcgacggaa agtgaagtga ctatcattac gccgatagtg gtgatcatgg
     4561 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
     4621 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
     4681 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
     4741 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
     4801 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgcgggcttt
     4861 tcagcagcag cacatgggta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
     4921 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttggtcact tccagcgtgg
     4981 cggcgaacgg gaacaggatc acctgaatca gcacgggtaa aatcagaatt gcgcgggttt
     5041 gcggttcgcg cagcagcgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
     5101 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
     5161 accgccgaag cgatcaaaaa cagcacgttc accaccagta ccaccggaat attcccggcg
     5221 aggaacaggc tttgcagggt gctgacgaaa taacgtgcgg gaataatgta cgtcaccgcg
     5281 cggatcaccg cgggcatact gtctatctga aatataaagc cggaaagcat aatcgacggc
     5341 agaaaagccg cgttcagggc cacctgagcg gcgttgaact ggttgcgggt aatcgtggaa
     5401 atcagcagcc ccatccccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
     5461 agcagcgacc cgcgatacgg cacgccgaga ataaacaccg acaccagcat acacagcaac
     5521 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ctccgtgcgg
     5581 gtaatctccg tagagagcaa agcctccatg gtgccgcgtt cccattctcg cgccaccacc
     5641 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc ggggataata
     5701 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
     5761 tcaaaagtct gcccgttatc ctccgcgcgc tgcatttgcc agatctgcca gatcccttcg
     5821 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
     5881 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccaccggaat aaccaccaga
     5941 ccgcgaattt tccccgcctg cattttggcg atcagttcct gacggttatc gctgatggtg
     6001 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
     6061 tgttccagta aaatcccgac gcgcagcttg ctggagtcga ggttaatgcc gtatccaaaa
     6121 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaaca
     6181 atctgccgcg tctctttgac gcacagcgcc cgcacgcgac gccaggacag gatcggatta
     6241 ctcattgcca tgctccttat cccagtcgtg gatcaactga ataaaggcct gctccattgt
     6301 gggatcgggt tgctcgtcgt tggccgactg cgctttcaaa tcatccggcg tgccgctggc
     6361 gattaatttc ccgcggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
     6421 gtgggtggtg accatcaccg tgacgccttt ctctaccata ctgttgatat gtagccaaaa
     6481 ttcacggcgg gtgaggggat caacgccgga cgtcggttcg tcgagaaaca gaatgtccgg
     6541 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
     6601 ggtcgcgtgg gaggcgatac tgttcaggcc gaacgcctcg ctcatacggg agattttttc
     6661 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
     6721 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
     6781 accagaactc tctttcagat ccatgcccag caccagcgcc tgaccagagg tcggtaccag
     6841 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggtc cgagcaaacc
     6901 aaaaatctcc ccgcgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
     6961 tttcttggtt agttccttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
     7021 taatattgcg cccagcggcg attccgaggt tccggcaccg cccagcaaat caataaacgc
     7081 atcttcaaaa cgcggcgtag tttcgttgat gtcgatttcc ggcatcccgt cggcatggcg
     7141 aatatcgtct ggcgtggtct cttttttgag gatcagacgt accgatttcc cctgaatcat
     7201 gccgtcgctg acctgcggca gtttcaatgc ccgttgcaac agtttgcggt tgccctcgtg
     7261 cgggctggtc atcagaaagc tgcgtccggc catggtttga gtcagggccg tcggttctcc
     7321 ctgatacagc agctcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
     7381 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
     7441 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
     7501 tttcggttcg cccaccaggg tacaggccag gccaagtttt tgtttcatcc caccggagag
     7561 cttgcccgcc aggcgtccgg taaacgggcc aagagacgta aactccagca ggcgagcaaa
     7621 agtttgcttc cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
     7681 catcaccgtc agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
     7741 cagcgcagcg tcatttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
     7801 tttcagtaat cccgccagca tccgcatcag cgtggttttg cccgcaccgt ccggcccaac
     7861 cagccccgtc acataaccgg cgtgaatggt gcagtcgagc ggtgcgacgg cgggcttgtc
     7921 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
     7981 cctcgtcacc gaattgcacc gttaccggca ttccctggcg taacgcatca tcggcgtcgg
     8041 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
     8101 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
     8161 ggcgaccatc ggtataaagc aacactttgc gccccggctg ggcctggtca agattacgtt
     8221 catcaacata ggcgcgtacc cacaccggac gcgtcagcga gacggtaaat accgtgccac
     8281 cttcattaag caccgtgccg ggctctaccg cgcgcgttaa cagcgtgcca tcagacggag
     8341 cgaccagcgt cgaatcctgc aaattcagct ccgcctgcgc cagttgcgcc tgcgcttgtt
     8401 cgaggctggc tttcgcctgg gcgatgtcct gttcgcggtt accggaacgg tactgacgca
     8461 atttatcctg cgctgatttc agcgtggcct gcgcctggtc gcgcgaggag cgggcatttt
     8521 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
     8581 tctgcgcata gtcataggca gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
     8641 cttcatctcg atacccggca agcatcaggt catactgcgc ctgagccacc gaaacgcccg
     8701 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
     8761 ccgctttgat cgcatcacct tcgtccaccg ccagcgactc aacgcgcccc ccaacacgga
     8821 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
     8881 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtactact accgccagtc
     8941 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
     9001 gtcaatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
     9061 ccagccggta cgtaacagaa tagtttcttt gccaagacga aacgccagaa tctcaccaat
     9121 caacgcatgg gtatgaagga tcatgcgggt atcgctagcg tcgcagccgg tccaggcggc
     9181 aatcaggcgt gtaaggtgac tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
     9241 ggctgccgtg ggagagagct gctcacggga gataaatttg ctgaggttga cggtgtcatc
     9301 ctgggtgagc agcttaatca tgtttctgca ggcgcgaagg atcagttcac ggatggctgc
     9361 ccgatccggc tgtggttgtg cgaacaaacg ttcggcttct tcggcgtgtg gacggaactg
     9421 ctcgccaata aaatcagcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
     9481 gaagtagtag gtgatggctg cgatattctg cccggcctgg gcggctatct cgcgggtggt
     9541 ggcgttcatt ccatattcac caaactgtgc cagtgccgca gcaatcagct gttttttcgc
     9601 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
     9661 gattgattaa gattatgact ccatagggga gttgtccagt atggctaaga attttagcta
     9721 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
     9781 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
     9841 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
     9901 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
     9961 cctgcggtgc tggaaggccg cgacctgatg gccagcgccc agaccggtac cggcaaaaca
    10021 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    10081 cgtcgtccgg tacgcgcgct cattcttacc ccgacccgtg aactggcggc gcagattggc
    10141 gaaaacgtcc gtgattacag caaatatctg aacattcgtt cgctggtggt gtttggcggt
    10201 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    10261 ccgggacgtc tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    10321 ctcgtccttg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    10381 gtgttaacaa aactgccagc gaagcgccag aacctgttgt tctccgcgac cttctctgac
    10441 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    10501 cgcaatactg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    10561 cgcgaattgc tgtcgcatat gattgggaaa gggaactggc agcaggtgct ggtgtttacc
    10621 cgtaccaaac atggcgctaa ccatctggct gaacagctta ataaagacgg catccgtagt
    10681 gcggcgatcc acggtaataa atcgcaaggt gcgcgtactc gtgcgctggc cgattttaaa
    10741 tcgggcgata ttcgtgtact ggtggcaact gacatcgctg cgcgcggtct ggatatcgaa
    10801 gagttgccgc acgtcgtcaa ctatgagtta ccaaacgtac cggaagatta tgttcaccgt
    10861 atcgggcgta ctggccgtgc ggcggcaact ggcgaagcat tgtcgctggt gtgtgttgat
    10921 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    10981 attccggggt atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    11041 caacgtggcg gcggtcgtgg acagggcggt ggtcgcggtc aacagcaacc acgccgtacg
    11101 gaaagtggtg cgaagtctgg taacgcgaag cccgcagaaa aaccgtctcg tcgtctcggc
    11161 gatgccaaac cggcaggcga acaacaacgt cgccgccgtc cgcgtaaacc tgccgctgcg
    11221 cagtaatctt ttatgccggg ctatgcccgg catgagactg atgacaaac
//
LOCUS       DAZFRG010000159        11257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFRG010000159 DAZFRG010000000
VERSION     DAZFRG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.159"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1270
                     /locus_tag="ACHKUC_003364"
     CDS             <1..1270
                     /locus_tag="ACHKUC_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6619567.1"
                     /translation="FGSFAVMLAIGVPITFAIGLSSLLSIITALPPDAAISVISQKMT
                     VGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLAHCNILANTLFGA
                     ISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPPSNVLIVYSLASG
                     GTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQFLQVFRESIPSLM
                     LIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDILLDSVVTSSIVLL
                     LVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIVGTFMDITPAILI
                     FTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSIGKVSIDRAIKPL
                     LPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(1341..2753)
                     /gene="ftsP"
                     /locus_tag="ACHKUC_003365"
     CDS             complement(1341..2753)
                     /gene="ftsP"
                     /locus_tag="ACHKUC_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO6619568.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(2828..3565)
                     /gene="plsC"
                     /locus_tag="ACHKUC_003366"
                     /gene_synonym="parF"
     CDS             complement(2828..3565)
                     /gene="plsC"
                     /locus_tag="ACHKUC_003366"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO6619569.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(3800..6058)
                     /gene="parC"
                     /locus_tag="ACHKUC_003367"
     CDS             complement(3800..6058)
                     /gene="parC"
                     /locus_tag="ACHKUC_003367"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO6619570.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAVIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQGYGDERRSPLHEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(6196..7803)
                     /locus_tag="ACHKUC_003368"
     CDS             complement(6196..7803)
                     /locus_tag="ACHKUC_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6619571.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(7912..8394)
                     /locus_tag="ACHKUC_003369"
     CDS             complement(7912..8394)
                     /locus_tag="ACHKUC_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO6619572.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLEMDVYVPLA
                     "
     gene            complement(8447..8839)
                     /gene="ygiW"
                     /locus_tag="ACHKUC_003370"
     CDS             complement(8447..8839)
                     /gene="ygiW"
                     /locus_tag="ACHKUC_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO6619573.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8991..9650
                     /gene="qseB"
                     /locus_tag="ACHKUC_003371"
     CDS             8991..9650
                     /gene="qseB"
                     /locus_tag="ACHKUC_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO6619574.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9647..10996
                     /gene="qseC"
                     /locus_tag="ACHKUC_003372"
     CDS             9647..10996
                     /gene="qseC"
                     /locus_tag="ACHKUC_003372"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO6619575.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAEQGGFEAKVSW"
     gene            complement(11042..>11257)
                     /locus_tag="ACHKUC_003373"
     CDS             complement(11042..>11257)
                     /locus_tag="ACHKUC_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO6619576.1"
                     /translation="DNICSIPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFE
                     FWLYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT         2661 a         2988 c         2830 g         2778 t
ORIGIN      
        1 atttggcagt tttgccgtca tgctggcaat aggtgtgcca attacttttg cgattggtct
       61 ttcttcgctg ttatctatta ttactgcctt gccacccgac gctgccattt ctgttatttc
      121 acaaaaaatg actgtagggc tggatggctt tacgctatta gccattccct tcttcgtgtt
      181 agccggaaac attatgaata ccggtggtat agccagacga ctggttaacc tggcgcaagc
      241 attagttggg cgtcttcctg gctcactggc tcattgtaat atcctcgcga atacactgtt
      301 tggtgcgatt tcaggttcag ccgttgcgtc agccgcagcg gtaggtggga ttatgtcacc
      361 actgcaagaa aaagagggat atgatccggc gttttcagca gcggttaata ttgcctctgc
      421 ccccattggc ctgatgatcc caccgagcaa tgtgttgatt gtttattccc tcgccagtgg
      481 cgggacttct gtcgctgcgt tgttcttagc tggatatctg ccgggcatcc tcaccgctgt
      541 tgccttaatg tttgtagctg cactttatgc ccgacgtcac cattatccgg tggccgagcg
      601 cattaattac cgtcagtttt tacaggtatt ccgcgaatca attcctagcc tgatgcttat
      661 ttttatcatt attggcggta ttatcgccgg ggtatttacg cctacagaag catcggcaat
      721 tgcggtaatt tatagtttag ccctggcgat gatttaccgg gaaatcactc ttaagaagct
      781 caatgatatt ctgttagatt cggtagtaac cagttcaatt gttctgttac tggtaggctg
      841 ctcgatgggg atgtcatggg ccatgacgaa tgctgatgtt cctgagttga tcaacgaact
      901 gattacccgc gtttcggata acaaatgggt cattctgttt attatcaata tcattctgtt
      961 gatcgtcggt acctttatgg atatcacgcc ggcgatcctg atatttacac ctattttcct
     1021 gccgatcgcc cagcatctgg ggattgatcc gattcacttc ggcatcatta tggttttcaa
     1081 cttaaccatt ggcctttgca caccacctgt cggcaccatt ctgtttgtgg ggtgcagcat
     1141 tggtaaggtc agtatcgaca gggcaataaa accattactg ccgatgtttc tggcattgtt
     1201 tgtagtaatg gcaattattt gttatttccc gcagcttagt ctgatgttgc caggattatt
     1261 ttcgacctga tacacaatga agactgcata ctgagtaatt ttcaatgccc caccactctg
     1321 tggggcattg aattggtcca ttacggtacc ggattgacca acagttgccc aatcgagcca
     1381 cggtccgcca tttccagcgt ctgactgttg aagtagaacg ggaagtgcgc ccaggaaggc
     1441 tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc aaacggtatc tttccagcct
     1501 ctgtcttccg ggaacggcat cgcgccattc acgttacgga tctggaacat cacgccttca
     1561 atatggaacg cttgcggctc gtccgcgcgc accgtccagc gttcccacgt tccttgctgc
     1621 gcagtaacat caatacggtt gacgtcccac aactgcccat taatccccgg gtcatcaccc
     1681 agactgatat cacgacttcg aatcggcgaa ccggccatga tttcagttgg cagcaagcgc
     1741 atcggaagac tgtcagtgac cagcggcaga aggccggttg ggcgcagcgt tagcaccaga
     1801 gtagaaacca gaatgctgga tggctcaaag aagccacgaa tacgatcaac aatgctcgcc
     1861 gcttcgccac aggtaatcga cacttcatca ccgttgctca tatccaccag aatctcacga
     1921 cgctcgcccg gtgccagcga aagttgcttc accgatacag gagcgggcag gaatccctga
     1981 tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct gtagttgata gcgacgagag
     2041 ttcgacgcgt tcaacagacg caagcgcacc cagccacgcg agacttcaac gtacgggctt
     2101 tgtacaccgt taaccagcag cgtatcacca acaaagccgc cgcttcccgg ttcgttgtat
     2161 tctggcgtac caaagttatc cagccgttta tcctgaataa tgaccggaaa atcatccaca
     2221 ccataatggt tggggatagg cagcgattta ctgacttcat cttccaccag ccacattccg
     2281 gcaaggccgt tatagacctg ctgagccgtg cggttgggag tattggcgtg ataccacaga
     2341 gtagctgcgt tctggcgaat gggcagtacg ggtgcccagt cagcgtttgg cgacatcatc
     2401 cgtgccggac cgcccatcag cgggccaggt acctgtagcc cggccaccgt cattgagaca
     2461 ttttctgtca ggcggttgct gtaaataagc ttaacatcgt cgcccttcca gacgcggata
     2521 gtcggcccca ggtaacgacc attgattccc caaaccgacg cgcgtgtccc tggcgtaaat
     2581 gaccagtgcg cacgttgtac agtcataaac agcggttgcc cacggcgaga ttcaagcagc
     2641 ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg ccttcagggg aacagcgcct
     2701 gcacaaagtg caatccccga tgcctgaatg aactgacgcc gactgagtga catatttgct
     2761 ccatgtaaaa ctggctaaag taataccgtg caggaaagtg ttccccgcaa aaatacagat
     2821 tgccgactta aacttttccg gctgcttcgc gttctgcgac ttctttatcg agctcggcga
     2881 ttttttgttc cattatcgaa cgacaatggg cagccaactc gcgaacctga tctttgccat
     2941 actgactgac gtcaattggc ggcagcattt cgacaatcac cagaccgttg tgtagccgat
     3001 taagattaat cttattcgaa gttgtagaga cgcacacggg tataatcggg acgcccgccg
     3061 caattgccgc gtgaaatgcc ccggtcttga acggcagcag gccgcgacca cggctgcggg
     3121 ttccttccgg gaacatccag atggaaatac ggcgtttttt gaagtgattc actacttccg
     3181 caatagtgcc gtgagcttta gtgcgattgt ttctgtcgat caataagttg ccggttaacc
     3241 agtacaactg cccgaagaag gggatccaca gcaagctctt tttacctacc gtcactgtcg
     3301 gcggctgcac aatgttcgat gccgtcacca tgtcataatt gttctggtgg ttagcgatat
     3361 agatagcatt gccgtagctt tcagcgtctg caggtttgcg gcactccact ttcaggccga
     3421 aaagcggcgc aagacgacca aacatatgcc caaaggtggc cacatgtttc gggttacgcg
     3481 ggctgaaaag gcagtaaatg gagccgaata cacagactaa gatgctgtaa atcacggtaa
     3541 taataagacg aaagatatat agcataacac cctctgaagg tctgagagac tggcattgta
     3601 cgtcacctga aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
     3661 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccggggattg
     3721 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
     3781 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
     3841 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
     3901 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
     3961 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc caggccatcc tcgccacgcg
     4021 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
     4081 gatcgcttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
     4141 aagcatcttc aatcaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
     4201 ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg
     4261 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
     4321 ctccaggcgg caacgttaat ttgccggtga gcggctcgcc ctgaccacgc gccgacggca
     4381 gcgtaatcgg gtcgatggca tagctacgac cggtggaatc aacaaacact accggttggt
     4441 tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
     4501 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
     4561 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcatgca
     4621 acggcgaacg acgttcgtca ccgtagcctt gcgcgtctgc ctgcagttct ttcttcagca
     4681 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
     4741 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
     4801 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
     4861 tcggttcatc ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca
     4921 gcaaaccttc gaggatatgc aggcgcttga gaactttctc cagacgatag ttcagtcggc
     4981 ggcgcacggt atcgcggcgg aacaccagcc attcggagag gatttccagc aggtttttca
     5041 ccgccggacg accatccaga ccgatcatat tgaggttaat gcgatagctc ttttccagat
     5101 cggtggtagc aaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
     5161 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
     5221 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
     5281 gatgcggcaa tgcgctgata accaccgcgc catcttcctt cttccaaacc gcgcgcatac
     5341 gcactgaacc acgtccgttc tcgtagattt tacgaatctc ggcgcgcgaa gtgataattt
     5401 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt
     5461 tcggctggtc gattaaggca attaccgcct gagccacttc acgcaggtta tgcggtggaa
     5521 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
     5581 gggcaggcag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
     5641 tcccctgccc cagctcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
     5701 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcccc ccagttcccc tgaccatcaa
     5761 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcac
     5821 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
     5881 atttggcgct ggcattcagg cccagctcag acatcgcata cacaatgcgg cgctgaacag
     5941 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
     6001 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
     6061 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
     6121 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
     6181 gcggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg
     6241 gggattatgc agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata
     6301 gtagatggga atcagcggtg cctgctggtt gatgatcact tctgcctgct gatacagcgc
     6361 attacgcttt gttgcatcag ccgtttgtgc cgcctggttt agtaaggcgt catactgcgc
     6421 gtttttccag tggccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga
     6481 ggcatcattg tacgtcgcat cccacgactg ccgagacagc ataaaatcac cggctcgtcg
     6541 ggcatcaaga taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt
     6601 tttccattcg gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa
     6661 cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca agacttttgc
     6721 catcgcgacg cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc
     6781 ttttacctct ggcggcgtca gcgtggttgc gggcgttctc aaccccagta ccttttgcgc
     6841 aataagctgt cgatcaaccg taagatatag cgcccgacgc acccgcacat cgttaaatgg
     6901 cggtttctca aggttaaagt tgtaatattc gctgttcaga cgcgggataa ttcgtagctc
     6961 gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac
     7021 ctctcccgcg cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac
     7081 ctgttgcaat actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt
     7141 ttcgttaact acccatttat caagcacaaa ggcaccgttg taaaccatgt tctctggctt
     7201 actccagcta tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca
     7261 ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc gtaacttcaa gagtacgatc
     7321 atccatcgct ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc
     7381 attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac
     7441 cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga ggctgaccgt ctgaccactg
     7501 caaaccgcta cgcagatgga aaatatagcg cttgccgccg tcaagtatct cccagcgttc
     7561 agcctgagcg ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc
     7621 cagcacaatc tgcgcggcgg tattctcctc taccttttgc gggtcgagcg tgcctgggtc
     7681 gctatgattg ttgtaacgaa agacttgttg cggggcgagc ggtgtgttgg cgggaacgtc
     7741 agcagcgtag agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata
     7801 catggtattc cttaccggag aaattataag cccgacaaca atacgcctgt ggcattgttt
     7861 gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc atcacgctaa
     7921 cggtacataa acatccattt ccagtcgctg ctctgtcatc ccttcggcaa gattggtgta
     7981 gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat
     8041 gatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acatggcggg ccacagcata
     8101 acgtccaccg gtaagttcac cattacttac gccataacga ttatcgggga tcggttcgct
     8161 aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca
     8221 ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat
     8281 gataaatttc gccacgctgt agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc
     8341 cacagggatt gatgggaaat cgataatatt tacatccagt gtcaggtttg tcattttgcc
     8401 tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg taacaattac ggatttactt
     8461 tgcggatctg tttgacgtca atttcaacag agttccagtc tttatcgact tcaccctgaa
     8521 tctcaaccgt atctttcggc gtcaccgtca cgccgttcca gcgtttatgg tcgatatcaa
     8581 cattgatagt accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga
     8641 tattgccgcg tagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg
     8701 ttacgctgcc gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag
     8761 aaaaacctcc ctgctctgct gccataaccg gtgcactgca cagggccatt actgcgatta
     8821 ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca
     8881 agatagtcct taacaacttc ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg
     8941 ttacaacacg gtttactggc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt
     9001 tactgataga agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg
     9061 gttttagcgt cgactggttt acacaaggtc gtcagggaaa agaggcgcta tatagcgcac
     9121 cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc
     9181 gcgaatggcg agaaaaaggt cagcgtgagc ctgtattgat cctgactgcg cgcgatgcgc
     9241 tggcggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg
     9301 cgttgataga agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca
     9361 gcaacgagct gcgccacggc aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg
     9421 ctggcgaacc cttaacgctg aaaccaaaag aatttgccct gctggaatta ctgatgcgta
     9481 acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag
     9541 aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg
     9601 attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca
     9661 acgtcttagt ctgcgagtca ggctgacgct aatcttttta attctggcct cggtgacctg
     9721 gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg agttgttcga
     9781 cacccaactg atgctgtttg ccaaacggtt aagtacgctc gatctcaacg aaatcaacgc
     9841 ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc
     9901 gctgactttt gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg
     9961 agaagatatt ccctatagct atcagcggga aggttttgct gacgggcaac tggtcggtga
    10021 agacgatccc tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt
    10081 gggtcaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg gacaattgat
    10141 cccgtggctg gtcgcactgc cgattatgtt aatcatcatg atggtgctac tgggtcgtga
    10201 actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgacccag actcggaaaa
    10261 accactaaac gcgacgggcg tacccagcga agtgcgccca ctggttgagt cgctaaatca
    10321 actgttcgcc cgcacacatg cgatgatggt tcgtgaacga cgctttacct ccgacgcagc
    10381 tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa accgaagttg ctcagctctc
    10441 tgacgatgat ccgcaggcgc ggaaaaaagc gctgctccaa ttacattccg ggatcgatcg
    10501 cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct
    10561 tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta
    10621 ccacacggcg cagcaggcga acattgacgt gcgactgaca ctcaatgcca acggcatcaa
    10681 acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt
    10741 gcgttacagt ccacaaggta gcgtggtaga cgtcacgctg aatgccgata atttcatcgt
    10801 gagggataac ggccctggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    10861 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    10921 cgccaaattg catggcatga atgttgattt tgggaatgcg gaacaaggtg gatttgaggc
    10981 gaaggtaagc tggtaacttc tctgtctatg agagccgtta aaacgactct catagacttt
    11041 actaatagca aaatataaac cgtccccaaa aaagccacca accacaaccc agacaaataa
    11101 cgagcgagaa tacatatagc caaaactcga acatttcccg cataaagagt tttcttaaga
    11161 taagaataat aagtggtgtc agaagaaaaa atgctgcaat cattgcttgt aatatacgat
    11221 cttcgcctga aacagaaggt atactgcaaa tattgtc
//
LOCUS       DAZFRG010000160        11138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFRG010000160 DAZFRG010000000
VERSION     DAZFRG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.160"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            35..139
                     /gene="speFL"
                     /locus_tag="ACHKUC_003374"
     CDS             35..139
                     /gene="speFL"
                     /locus_tag="ACHKUC_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO6619577.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            449..2647
                     /gene="speF"
                     /locus_tag="ACHKUC_003375"
     CDS             449..2647
                     /gene="speF"
                     /locus_tag="ACHKUC_003375"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO6619578.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2644..3963
                     /gene="potE"
                     /locus_tag="ACHKUC_003376"
     CDS             2644..3963
                     /gene="potE"
                     /locus_tag="ACHKUC_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO6619579.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4020..5660)
                     /gene="pgm"
                     /locus_tag="ACHKUC_003377"
     CDS             complement(4020..5660)
                     /gene="pgm"
                     /locus_tag="ACHKUC_003377"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO6619580.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLASEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5686..6231)
                     /gene="seqA"
                     /locus_tag="ACHKUC_003378"
     CDS             complement(5686..6231)
                     /gene="seqA"
                     /locus_tag="ACHKUC_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO6619581.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6416..7180
                     /gene="ybfF"
                     /locus_tag="ACHKUC_003379"
     CDS             6416..7180
                     /gene="ybfF"
                     /locus_tag="ACHKUC_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6619582.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7320..7613
                     /gene="ybfE"
                     /locus_tag="ACHKUC_003380"
     CDS             7320..7613
                     /gene="ybfE"
                     /locus_tag="ACHKUC_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO6619583.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7753..8283
                     /gene="fldA"
                     /locus_tag="ACHKUC_003381"
     CDS             7753..8283
                     /gene="fldA"
                     /locus_tag="ACHKUC_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO6619584.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8493..8579
                     /gene="uof"
                     /locus_tag="ACHKUC_003382"
     CDS             8493..8579
                     /gene="uof"
                     /locus_tag="ACHKUC_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO6619585.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8572..9018
                     /gene="fur"
                     /locus_tag="ACHKUC_003383"
     CDS             8572..9018
                     /gene="fur"
                     /locus_tag="ACHKUC_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO6619586.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9102..9428)
                     /gene="chiQ"
                     /locus_tag="ACHKUC_003384"
     CDS             complement(9102..9428)
                     /gene="chiQ"
                     /locus_tag="ACHKUC_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO6619587.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9478..10884)
                     /gene="chiP"
                     /locus_tag="ACHKUC_003385"
     CDS             complement(9478..10884)
                     /gene="chiP"
                     /locus_tag="ACHKUC_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO6619588.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAIFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2723 a         2777 c         2760 g         2878 t
ORIGIN      
        1 taccttcaac tgacacagaa aaaggtaaag gtaaatggaa aataataacc gcttaatgcc
       61 tcatataagg cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca
      121 tttctttaat gcccgctagt cttctgacta aagggcaccc cagcgtacag gtctccctga
      181 ctttaagcat tacaggttaa tatctgtatc cctcggtgct catatactgc taaccctctt
      241 aaactctaaa taattcgagt cgcagcacat gcaacttgaa gtatgacgag tatagccagt
      301 taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc
      361 ggtggggttt tgcttttttc cggcgtgatc aatttctcct ttgagaaatt gaggacctgc
      421 tattacctga aataaagaga tgaacaaaat gtcagaatta aaaattgccg ttagccgttc
      481 ttgcccggat tgtttttcca ctcatcgtga atgcgtgaat attgacaaaa gtaattatat
      541 tgacgttgcc gccattattt tatcagtcaa tgatgttgaa cgtggaaaac tcgatgaaat
      601 agacgctact ggctatggca ttcctgtttt tattgcaacg gaaaatgaag aacgtgtacc
      661 agcagaatat ctgccacgta tttctggtgt cttcgagcat tgcgaatcac ggaaagagtt
      721 ttatggacgt cagttagaaa ccgctgccag ccattatgaa acgcaactgc gcccgccctt
      781 cttccgcgca ctggtcgatt atgtcaatca gggcaatagc gcttttgact gcccgggtca
      841 tcagggaggt gagtttttcc gtcgccatcc ggcaggcaat cagttcgtgg aatattttgg
      901 cgaaatgctc ttccgctctg acttgtgtaa tgccgacgtt gcaatgggtg atcttctgat
      961 tcatgaaggc gcgccatgca ttgcacagca acatgcggca aaagtgttta atgccgataa
     1021 aacgtacttt gttttaaacg gcacctcctc ctccaacaaa gtggttttaa acgccctgct
     1081 gacaccaggt gatctggtgc tgtttgaccg caataaccat aagtctaacc atcacggggc
     1141 cttgctacag gctggtgcaa caccggttta tctggaaaca gcacgtaacc cgtatggttt
     1201 tatcggtggc attgatgcgc actgcttcga agaaagttat ctgcgtgaac tgattacaga
     1261 agttgcaccg caacgggcaa aagaggcgcg tccgttccgc ctcgctgtga ttcagttagg
     1321 cacctacgac ggtactattt ataacgcccg ccaggtggtg gataaaatcg gtcatctgtg
     1381 tgactacatc ctgtttgact cagcatgggt cggctatgaa cagtttattc cgatgatggc
     1441 ggactgttcg ccactgctgc tggagcttaa tgagaacgat ccgggtattc tggttacgca
     1501 atctgtgcat aaacaacagg ctggtttttc tcagacttca caaattcata aaaaagacag
     1561 ccatatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg catttatgat
     1621 gcacgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat
     1681 gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggttaacg gtattaatgc
     1741 ccgcaaattg atcctcgata actgccagca cattcgcccg ttcgttcctg aactggtgga
     1801 tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttctttaa
     1861 atttgtaccc ggtgagcact ggcattcttt tgaaggctac gcagagaacc aatactttgt
     1921 cgatccgtgc aaactgttgc tgacaactcc aggtattgat gcacgtaacg gcgaatatga
     1981 agcgttcggt gtacccgcaa cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc
     2041 ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa
     2101 actgcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctactggaag ctgatgcgcc
     2161 tctggcggaa gtactgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac
     2221 cctgcgtcag ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg tgaaacaact
     2281 gcaaaaagag atgttccgta aggaacactt cccacgcgtc agcatgaatc cgcaagaagc
     2341 caactacgcc tacttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg
     2401 tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttcctggtga
     2461 aatctggggt ggtgctgttc tgcgttactt cagcgctctg gaagaaggca tcaacctgct
     2521 gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca
     2581 agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc gccctgttga aaggggaaaa
     2641 attatgagtc aggctaaatc gaacaaaatg ggtgtcgttc agttaaccat actgacgatg
     2701 gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga agtcgggaca
     2761 atctcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc
     2821 gcaaaatgcg gtatgttcag ccgtaaatca ggaggtatgg gcggttatgc cgagtatgct
     2881 ttcggtaaat ccggtaactt tatggcgaac tatacctacg gcgtctcgct gctgattgct
     2941 aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg
     3001 tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac
     3061 tttggtggtg cgcgcattac cgggcaaatc agtagcatta ccgtgtgggg ggtcattatt
     3121 ccggtggtag gcctgtgcat tatcggctgg ttctggttta gcccgacact ttacgttgat
     3181 tcctggaatc cgcatcatgc accgttcttc agtgcggtag gttcttccat cgctatgacg
     3241 ctgtgggctt ttcttggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg
     3301 gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc
     3361 gtctccacca acgtgattgc cgggattgtg ccaaatatgg agctggcaaa ttcaacggca
     3421 ccatttggtc tggccttcgc gcagatgttc acgccggaag tgggtaaagt cattatggcg
     3481 ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg
     3541 tttaaatctt catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg
     3601 gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg
     3661 acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc
     3721 aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat
     3781 gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtacagc
     3841 ttttatgcgc tgtactcatc cggggaagaa gccatgctat acggttccat cgtgaccttc
     3901 ctcggttgga cactgtatgg tctggtctca ccacgctttg aactgaaaaa taaacacggt
     3961 taattaagca aagcaaaaaa gggcgatctt ccgaccgccc tttttcttat ttgatatgtt
     4021 tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga
     4081 tgttcttcac caaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga
     4141 cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccgataga agcgccatta
     4201 cccggtgccg ccgtcagacg cgcggtgatc gggtcacctg ccagggtgct ggcgctcacc
     4261 atttccggag acagcttaga caacgccgct ttttgtgcgg aagtcgctgc tgcctgcaaa
     4321 cggttatagc tcggcgcacc aaagcgtttt gccagttcgt tatagtgttc ctgcgggttc
     4381 ttaccggtga cagcggtgat ttccgacgcc agcagacaca taatgatgcc gtctttatcg
     4441 gtggaccacg gcgtgccgtc gaaacgcagg aaggaagcgc ctgcactctc ttcgccgcca
     4501 aagccgaagc tgccatcgaa caaaccatca acaaaccatt tgaaacctac cggtacttct
     4561 accagcttac ggcccaaatc attgaccaca cggtcgatca tcgcagatga aaccagcgtt
     4621 ttaccgacag caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc
     4681 accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca
     4741 taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag caggcccgcc
     4801 atcgcacact cggaggagca gtccatacgg atcgcaccgt ctttatcgag gtgcataaag
     4861 cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca
     4921 atgcgcttcc agtattcgat accggaacca cccagcggat caacgcccag cgtcaggccc
     4981 gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacaaa cggctgcacc
     5041 agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg
     5101 cctttcaggc catcagccag cagtgcgttt gctctgtctt ccaccacttt agtgacgttg
     5161 gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta
     5221 tgggacggtg taatcacgat accgtctgcc agcgggccac cttttttatt gtgaaccaga
     5281 atggcattgg aaatggcagg tgtcggagtg aagccattgt tttcctgcac aatgacatca
     5341 acgccgttcg ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga
     5401 gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt
     5461 gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg
     5521 tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca
     5581 taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct
     5641 gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca
     5701 aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct
     5761 gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtaccag gcacatgttt
     5821 cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg
     5881 tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag
     5941 tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtccga
     6001 aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc
     6061 gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc
     6121 ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt
     6181 gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca
     6241 gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc cggcagcgga
     6301 tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg
     6361 gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa
     6421 attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt
     6481 ccatggtctg tttggcagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga
     6541 tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat
     6601 gaattacccg gcgatggcgc aggatcttgt tgataccctg gatgcacagc agatcgacaa
     6661 agcaacattt atcggtcact ccatgggcgg taaagcggta atggctctta ctgcactagc
     6721 ccccgatcgc attgacaagc tggtggcgat tgatattgcg ccggtcgatt atcacgttcg
     6781 ccgtcatgat gagattttcg cggccatcaa cgcagtcagt gaatcagacg cacaaacacg
     6841 ccagcaagca gcggcaataa tgcgccagca tcttaatgaa gaaggggtga ttcagtttct
     6901 gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccagtattgt gggatcagta
     6961 tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc
     7021 tggcggcaat tctccgtatg ttagcgagca gtaccgcgat gatttactgg cacaatttcc
     7081 acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga
     7141 tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca
     7201 gcgtccgcgt cgctttccgc gcgtggacgt tggcgtgact catcgcgctg atgtattatg
     7261 gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca
     7321 tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg
     7381 gacgaccgaa aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc
     7441 agctaaaacg cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag
     7501 ctgtcgaggc gctgaacgag ctggcggaat cgcgcaatat gagccgtagc gaactgatcg
     7561 aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca
     7621 ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc
     7681 tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag
     7741 gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa
     7801 aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt
     7861 gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc
     7921 tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt
     7981 gatttcaatg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa
     8041 tatttctgcg acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaaccatc
     8101 gttggtcact ggccaactgc gggctaccat ttcgaagcgt caaaaggtct ggcagatgac
     8161 gaccattttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt
     8221 gtagaaaaat gggttaaaca gatttctgaa gagctgcatc tcgacgaaat tctcaatgcc
     8281 tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat
     8341 aattgctaca aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg
     8401 gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact
     8461 tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt
     8521 ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat
     8581 aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgctt gaaaatcctg
     8641 gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg
     8701 atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac
     8761 gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactaaca
     8821 cagcaacatc accacgatca cctgatctgc ctcgactgcg gtaaggttat cgaatttagt
     8881 gatgattcca tcgaagcgcg tcagcgtgaa atcgccgcaa aacatggcat tcgcctgact
     8941 aaccacagtc tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgagcac
     9001 gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc aacctgcagg ttggcttttt
     9061 tatacaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt attactgcga
     9121 atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt
     9181 gcctgaatac actgctgata atccagtacg cggacacttt cctgctcggc aaactgctga
     9241 tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat tttttccggc
     9301 gaaccttgcg cggtgttgat acaggcgctg taagcctctt tcagacgact gtcttcctgc
     9361 ggtgcggttg attgcgcaca agctaccagc cccgatgcca tcatggcgat gagaatcagt
     9421 tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg tcgggcatca
     9481 gaagatggtg aatggtgcga ttaccatgaa tttcacgtcg cgctcatcct ggaaaatgtt
     9541 gccgtaacca ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
     9601 tttgaacatc gtgcccttgg cacgaccgtc ctgaatggta tacaccgcat ccaggctgta
     9661 ggcagactct tcaatagtcc ggtttttatc gtagtacgca tccggattgc tctgccaggt
     9721 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
     9781 ccagtttttc aggtcataca tcgcaccgaa gaagatcgct ttttcgccgt tggcgttgaa
     9841 gtcagaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt aggttggagt
     9901 catacgttgc aggaagtatc cttgctgacc gtcagcctta acccaggtac cttcgaggcg
     9961 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcctgcagcc aggcggtgcc
    10021 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccat agaactggta
    10081 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    10141 atcgatatac ccttcggcct gaccaaacgc cgcttccagt acgaagttat ttttgaagtc
    10201 gtacttcgcc ccaagggagt gtagataatc aactttggtg gttttatcgt tctgataaaa
    10261 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    10321 caacgcacca gcatcgccgt aatcaaaatt cgccccggcc tccgccccct gataagtacc
    10381 cggcataaag ctccagtgtg gcgctaacag cgtctgaccg gttggctgaa tataacctgc
    10441 cctcgcccaa accggaccgt atttaaattt ggctgctgct ttatacaggc ttataccgct
    10501 tttatcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    10561 cgggtgagag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcgg caatatcaag
    10621 gccgaacata tcagcagcat aaccagactg aaaatcgagg ttggcgttcc aggtggagtg
    10681 agaaaggttg gttttgtatt tatcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    10741 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    10801 cggaattgcc ataaagcccg acatagctgt aacaccggcg atagccagcg ccagcgtact
    10861 acgtttgcca ctaaacgcac gcatgggtta atcctctttg acgtataaat tgctgcacca
    10921 aaggtgaaca gcgaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac
    10981 tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt
    11041 aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcagt
    11101 tgaaaggctt gcagaagaat aaaaacgtga aattccag
//
LOCUS       DAZFRG010000161        11060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFRG010000161 DAZFRG010000000
VERSION     DAZFRG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.161"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            328..708
                     /gene="atpI"
                     /locus_tag="ACHKUC_003386"
     CDS             328..708
                     /gene="atpI"
                     /locus_tag="ACHKUC_003386"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO6619589.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            717..1532
                     /gene="atpB"
                     /locus_tag="ACHKUC_003387"
     CDS             717..1532
                     /gene="atpB"
                     /locus_tag="ACHKUC_003387"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO6619590.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1579..1818
                     /gene="atpE"
                     /locus_tag="ACHKUC_003388"
     CDS             1579..1818
                     /gene="atpE"
                     /locus_tag="ACHKUC_003388"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO6619591.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1880..2350
                     /gene="atpF"
                     /locus_tag="ACHKUC_003389"
     CDS             1880..2350
                     /gene="atpF"
                     /locus_tag="ACHKUC_003389"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO6619592.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2365..2898
                     /gene="atpH"
                     /locus_tag="ACHKUC_003390"
     CDS             2365..2898
                     /gene="atpH"
                     /locus_tag="ACHKUC_003390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO6619593.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2911..4452
                     /gene="atpA"
                     /locus_tag="ACHKUC_003391"
     CDS             2911..4452
                     /gene="atpA"
                     /locus_tag="ACHKUC_003391"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO6619594.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4503..5366
                     /gene="atpG"
                     /locus_tag="ACHKUC_003392"
     CDS             4503..5366
                     /gene="atpG"
                     /locus_tag="ACHKUC_003392"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO6619595.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5393..6775
                     /gene="atpD"
                     /locus_tag="ACHKUC_003393"
     CDS             5393..6775
                     /gene="atpD"
                     /locus_tag="ACHKUC_003393"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO6619596.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6796..7215
                     /locus_tag="ACHKUC_003394"
     CDS             6796..7215
                     /locus_tag="ACHKUC_003394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO6619597.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7568..8938
                     /gene="glmU"
                     /locus_tag="ACHKUC_003395"
     CDS             7568..8938
                     /gene="glmU"
                     /locus_tag="ACHKUC_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO6619598.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9099..10928
                     /gene="glmS"
                     /locus_tag="ACHKUC_003396"
     CDS             9099..10928
                     /gene="glmS"
                     /locus_tag="ACHKUC_003396"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO6619599.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2658 a         2753 c         3008 g         2641 t
ORIGIN      
        1 tatcataaac ttgcaaaggc atcatttgcc aagtaaataa atatgctgtg cgcgaacatg
       61 cgcaatatgt gatctgaagc acgctttatc accagtgttt acgcgttatt tacagttttt
      121 catgatcgaa cagggtttgc agaaaagtcg caattgtatg cactggaaaa atatttaaac
      181 atttattcac cttttggcta cttattgttt gaaatcacgg gggcgcaccg tataatttga
      241 ccgctttttg atgcttgact ctaagcctta aagaaagttt tatacgacac gcggcatacc
      301 tcgaagggag caggagtgaa aagcgtgatg tctgtgtcgc tcgtgagtcg aaacgttgct
      361 cggaagcttc tgctcgttca gttactggtg gtgatagcaa gtggattgct gttcagcctc
      421 aaagacccct tctggggcgt ctctgcaata agcgggggcc tggcagtctt tctgcctaac
      481 gttttgttta tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg
      541 gcctggacat tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg
      601 gtggcgttgg cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg
      661 ctggtggttc agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg
      721 cttcagaaaa tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg
      781 acctgcgtac attctcgctg gtggatccac ataacccccc agccaccttc tggacaatca
      841 atattgactc catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta
      901 gtgtagccaa aaaggcaacc agcggcgtgc caggtaagtt tcagaccgcg attgagctgg
      961 tgatcggctt tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg
     1021 ctccgctggc cctgacgatc ttcgtctggg tattcctgat gaacctgatg gatttactgc
     1081 ctatcgacct gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg
     1141 ttccgtctgc tgacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc
     1201 tgttctacag catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc
     1261 cgttcaatca ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt
     1321 ccaaaccagt ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt
     1381 tcattctgat tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg
     1441 ccattttcca catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg
     1501 tctatctgtc gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga
     1561 aacaaactgg agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct
     1621 gtgatgatgg gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa
     1681 ttcctggaag gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt
     1741 atcgttatgg gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg
     1801 atgttcgctg tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac
     1861 taaatagagg cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt
     1921 tgtcctgttc gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga
     1981 aaaacgtcaa aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct
     2041 tgaccttgca aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt
     2101 aatcatcgag caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc
     2161 agaacaggaa cgtactaaaa tcgtggccca ggcgcaggcg gaaattgaag ccgagcgtaa
     2221 acgtgcccgt gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa
     2281 gatcatcgaa cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc
     2341 tgaactgtaa ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc
     2401 aaagcagctt ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg
     2461 gcgtttgccg ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt
     2521 gcgccagaaa cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac
     2581 ggtcagaacc tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt
     2641 ctggagcagt ttattcacct gcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt
     2701 tccgctgccg cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt
     2761 ctgtcacgca aagttaagct gaattgcaaa atcgataagt ctgtaatggc aggcgttatc
     2821 atccgagcgg gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca
     2881 gacgtcttgc agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa
     2941 ctgatcaagc agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact
     3001 attgtttctg taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt
     3061 gaaatgatct ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct
     3121 gtaggtgcgg ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt
     3181 actggccgta tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact
     3241 ctgggtgcac caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa
     3301 gcaatcgctc cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat
     3361 aaagccgttg actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac
     3421 cgtcagaccg gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt
     3481 atcaaatgta tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt
     3541 aaactggaag agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa
     3601 tccgcagcac tgcaatacct ggcaccgtac gctggttgcg caatgggtga atacttccgt
     3661 gaccgcggtg aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac
     3721 cgtcagatct ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc aggcgacgta
     3781 ttctacctcc actctcgtct gctggagcgt gctgcgcgtg ttaacgccga atacgttgaa
     3841 gctttcacca aaggtgaagt gaaagggaaa accggttctc tgactgcgct gccgattatc
     3901 gaaactcagg cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat
     3961 ggtcagatct tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg
     4021 ggtatttccg tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc
     4081 ggtggtatcc gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca
     4141 tccgaccttg acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg
     4201 ctgaagcaga aacagtatgc gccgatgtct gttgcgcaac agtctctggt tctgttcgca
     4261 gcagaacgtg gttacctggc ggatgttgaa ctgtcgaaaa tcggcagctt cgaagccgct
     4321 ctgctggctt acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt
     4381 ggctacaacg acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc
     4441 caatcctggt aacgtctggc ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc
     4501 tcatggccgg cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga
     4561 tcactaaagc gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg
     4621 cggccagccg tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta
     4681 atctggaata taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg
     4741 tggtgtcgac cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc
     4801 tggcggaaat gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct
     4861 cgaaaggcgt gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca
     4921 tgggggataa cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct
     4981 acgacgaagg ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt
     5041 ctcaggttcc gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga
     5101 aacataaatc ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc
     5161 tgcgtcgtta tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc
     5221 aggccgcccg tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag
     5281 agctgcagtt ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga
     5341 tcgtctcggg ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac
     5401 tggaaagatt gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt
     5461 accgcgcgtg tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt
     5521 tcagcagcag ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct
     5581 gcgtcgcggt ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc
     5641 gactctgggc cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg
     5701 tgaagaagag cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc
     5761 tcaggaactg ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg
     5821 cggtaaagtt ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct
     5881 tattcgtaac atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg
     5941 tactcgtgag ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt
     6001 atccctggtg tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac
     6061 cggtctgacc atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga
     6121 caacatctat cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc
     6181 ttcagcggta ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat
     6241 cacctccacc aaaaccggtt ctatcacctc cgtacaggca gtatacgtac ctgcggatga
     6301 cttgactgac ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag
     6361 ccgtcagatc gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg
     6421 tcagctggac ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc
     6481 catcctgcaa cgttatcagg aactgaaaga cattatcgcc atcctgggta tggatgaact
     6541 gtctgaagaa gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca
     6601 gccgttcttc gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga
     6661 caccatccgt ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc
     6721 gttctacatg gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct
     6781 taatcggagg gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa
     6841 atgttctctg gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc
     6901 taccctggcc acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa
     6961 cagcacggtc acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc
     7021 aacgtgaccg ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc
     7081 atggaagcga aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac
     7141 gctcaggcgt ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc
     7201 aaaaaagcga tgtaacaccg gcttgaaaag cacaaaagcc agtctggcaa caggctggct
     7261 ttttttacgc gtattgaccc gtcctgaaaa gcgttcacat agatcctgat gatataaaac
     7321 acccctgttt tcccgtttat tcattgatcg aaataagagc aaaaacatcc acctgacgct
     7381 taaatgaagg tactgcctta attttctgca gacaaaaggc gtgacgatgg tcgaaaatgg
     7441 cgctttcgtc agcggggata atccgttatt gaacaattta tcctctgtcc atttcacgat
     7501 gaaaaaaatg tagttttttc aaggtgaagc ggtttaaatt cgttctcaaa ttacagtcag
     7561 gacgcgtatg ttgaataatg ctatgagcgt agtgatcctt gccgcaggca aaggcacgcg
     7621 catgtattcc gatcttccga aagtgctgca tacccttgcc gggaaagcga tggttcagca
     7681 tgtcattgat gctgcgaatg aattaggcgc agcgcacgtt caccttgtgt atggtcacgg
     7741 cggcgatctg cttaagcagg cgctgaaaga cgacaacctg aactgggtgc ttcaggcaga
     7801 acagctgggt acgggtcatg cgatgcagca ggccgcacct ttctttgccg atgatgaaga
     7861 cattttaatg ctctacggcg acgtgccgct gatctctgtc gaaacactcc agcgtctgcg
     7921 tgatgctaaa ccgcagggtg gcattggtct gctgacggta aaactggatg atccgaccgg
     7981 ttatggacgt atcacccgtg aaaacggcaa agttaccggc attgttgagc acaaagatgc
     8041 caccgacgag cagcgtcaga ttcaggagat caacaccggc attctgattg ccaacggcgc
     8101 agatatgaaa cgctggctgg cgaagctgac caacaataat gctcagggcg aatactacat
     8161 caccgacatt attgcgctgg cgtatcagga aggacgtgaa atcgtcgccg ttcatccgca
     8221 acgtttaagc gaagtagaag gcgtgaataa ccgcctgcaa ctctcccgac tggagcgcgt
     8281 ttaccagtcc gaacaggctg aaaaactgct gttagcaggt gttatgctgc gcgatccggc
     8341 gcgttttgat ctgcgcggta cgcttactca cgggcgcgat gttgaaattg atactaacgt
     8401 tatcatcgag ggcaacgtga ctctcggtca tcgcgtgaaa atcggcaccg gttgcgtgat
     8461 taaaaacagc gtgattggcg atgattgcga aatcagtcca tataccgtcg tggaagacgc
     8521 gaatctggca gcggcctgta ctattggccc gtttgcccgt ctgcgtcctg gtgctgagtt
     8581 gctggaaggt gcacacgtcg gtaacttcgt tgagatgaaa aaagcgcgtc tgggtaaagg
     8641 ctcgaaagct ggtcatctga cttacctggg cgatgcggaa attggcgata acgttaacat
     8701 cggcgcggga accattacct gcaactacga tggtgcgaat aaatttaaga ccattatcgg
     8761 cgacgatgtg tttgtcggtt ccgacactca actggtggcc ccggtaacag taggcaaagg
     8821 cgcgaccatt gctgcgggta caactgtgac gcgtaatgtc ggcgaaaatg ccctggcgat
     8881 cagccgtgtg ccgcagactc aaaaagaagg ctggcgtcgt ccggtaaaga aaaagtaatt
     8941 ctggccgggt aatccggtca tatgggatga ggagataaca taatctcccg cccacaagca
     9001 gtaactataa aaataacccc actctctaca aggctcgggg cgcccgaaaa acgggcatac
     9061 aggttgaccg acaacgatat aaatcggaat caaaaactat gtgtggaatt gttggcgcga
     9121 tcgcgcaacg tgatgtagca gaaatccttc ttgaaggttt acgtcgtctg gaataccgcg
     9181 gatatgattc tgccggtctg gccgttgttg atgcagaagg tcatatgacc cgcctgcgtc
     9241 gcctcggtaa agtacagatg ctggctcagg cagcggaaga acatcctctg catggcggca
     9301 ccggtattgc tcacactcgc tgggcgaccc acggtgaacc ttcagaagct aacgcgcatc
     9361 cgcatgtttc tgaacacatt gtggtggtgc ataacggcat catcgaaaac catgaaccgc
     9421 tgcgtgaaga gttaaaagcg cgtggctata ccttcgtttc tgaaaccgac accgaagtga
     9481 ttgcacattt ggtgaactgg gagctgaaac aaggcggtac tctgcgtgag gcagttctgc
     9541 gtgctatccc gcaactgcgt ggtgcgtacg gtacagtgat catggactcc cgtcatccgg
     9601 ataccctgct ggcggcacgt tctggtagtc cgctggtgat tggcctgggt atgggcgaaa
     9661 actttatcgc ttctgaccag ctggcgctgt tgccagtgac tcgtcgcttt atcttccttg
     9721 aagagggcga tattgcggaa atcactcgcc gttcggtaaa catcttcgat aaaactggcg
     9781 cggaagtaaa acgtcaggat atcgaatcca atctgcaata tgacgcgggc gataaaggta
     9841 tttaccgcca ctacatgcag aaagagatct acgaacagcc gaacgcgatc aaaaacaccc
     9901 ttaccgggcg catcagccac ggtcaggttg atttaagcga gctgggaccg aacgccgatg
     9961 aactgctgtc gaaggttgag catattcaga tcctcgcctg tggtacttct tataactccg
    10021 gtatggtttc ccgctactgg tttgaatcgc tggcaggtat tccgtgcgac gtcgaaatcg
    10081 cctctgaatt ccgctatcgc aaatctgccg tgcgtcgtaa cagcctgatg atcaccttgt
    10141 cacagtctgg cgaaaccgcg gataccctgg cgggcctgcg tctgtcgaaa gagctgggtt
    10201 accttggttc gctggcaatc tgtaacgttc cgggttcttc tctggtgcgc gaatccgatc
    10261 tggcgctgat gaccaacgcc ggtacggaaa ttggcgtggc ctccaccaaa gcattcacca
    10321 cccagttaac tgtgctgttg atgctggtgg cgaaactgtc tcgcctgaaa ggtctggatg
    10381 cctccattga acatgacatc gtgcatggtc tgcaggcgct gccgagccgt attgagcaga
    10441 tgctgtctca ggacaaacgc attgaagcgc tggcagaaga tttctctgac aaacatcacg
    10501 cgctgttcct gggccgtggc gatcagtacc caatcgcgct ggaaggtgca ttgaagctga
    10561 aagagatctc ttacattcac gctgaagcct acgctgcagg cgaactgaaa cacggtccgc
    10621 tggcgctgat tgatgccgat atgccggtta tcgtcgttgc accgaacaac gaattgctgg
    10681 aaaaactgaa atccaacatt gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg
    10741 ccgatcagga tgcgggtttt gtgagcagtg acaatatgca cattattgag atgccgcatg
    10801 tggaagaggt gattgcaccg atcttctaca ccgttccgct gcaactactg gcttaccacg
    10861 ttgcgctgat caaaggcacc gacgttgacc agccgcgtaa cctggcaaaa tcggtaacgg
    10921 ttgagtaata aatggatgcc ctgcgtaagc ggggcatttt tcttcctgtt atgtttttaa
    10981 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca
    11041 cagaatgaaa atttttctgt
//
LOCUS       DAZFRG010000162        10870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFRG010000162 DAZFRG010000000
VERSION     DAZFRG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.162"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..822
                     /gene="adiY"
                     /locus_tag="ACHKUC_003397"
     CDS             61..822
                     /gene="adiY"
                     /locus_tag="ACHKUC_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO6619600.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            959..2296
                     /gene="adiC"
                     /locus_tag="ACHKUC_003398"
     CDS             959..2296
                     /gene="adiC"
                     /locus_tag="ACHKUC_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO6619601.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2400..4043
                     /gene="eptA"
                     /locus_tag="ACHKUC_003399"
     CDS             2400..4043
                     /gene="eptA"
                     /locus_tag="ACHKUC_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO6619602.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4040..4708
                     /gene="basR"
                     /locus_tag="ACHKUC_003400"
     CDS             4040..4708
                     /gene="basR"
                     /locus_tag="ACHKUC_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO6619603.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4709..5809
                     /gene="pmrB"
                     /locus_tag="ACHKUC_003401"
     CDS             4709..5809
                     /gene="pmrB"
                     /locus_tag="ACHKUC_003401"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO6619604.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="ACHKUC_003402"
     CDS             complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="ACHKUC_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO6619605.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5986..7488)
                     /gene="proP"
                     /locus_tag="ACHKUC_003403"
     CDS             complement(5986..7488)
                     /gene="proP"
                     /locus_tag="ACHKUC_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO6619606.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7752..8630)
                     /gene="rdcB"
                     /locus_tag="ACHKUC_003404"
     CDS             complement(7752..8630)
                     /gene="rdcB"
                     /locus_tag="ACHKUC_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO6619607.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8627..10855)
                     /gene="crfC"
                     /locus_tag="ACHKUC_003405"
     CDS             complement(8627..10855)
                     /gene="crfC"
                     /locus_tag="ACHKUC_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO6619608.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TELPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     NSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIAQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2733 a         2863 c         2656 g         2618 t
ORIGIN      
        1 tcccggtatc gcttcgcttt agcgagaact ggtcttttat tcgcactcag gagtacatgt
       61 atgaggattt gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata
      121 agggtgaatg ggaaagaacc tattagcctt aaagctaacc acatggcgtt attaaattgt
      181 gaaaataata ttatcgacgt ctcctctctt aataacactt tggttgctca tattagtcac
      241 gacatcatca aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg
      301 caacgtagcg ctacgcccat cctcaccctg ccatgcctga cgccagacgt ctttcgcgtt
      361 gccgcgcagc atagcatgat gcccgctgaa actgagtcag aaaaggaacg aacacgtgca
      421 ttattattca ctgtgctatc ccgttttctc gacagtaaaa aattcctttc actaatgatg
      481 tatatgttac gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatatccac
      541 aaagactgga atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag
      601 aaaaagctga aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgc
      661 tatgccgtaa atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc
      721 ggctacaaca gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg
      781 ctgcattatg ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag
      841 agctatatta ataacggcat cagcgataac ccggccgata ataattcaac tatcgaatgc
      901 aggcgtatga tacgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat
      961 gtcttcggat gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg
     1021 gaatattatg gggtcaggtg tttttctgtt acctgcaaac ctggcctcca ctggcgggat
     1081 tgccatttat ggatggttgg tgacgattat cggtgcgctg gggctctcga tggtatacgc
     1141 caaaatgtcg ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt
     1201 tggcccgttt ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa
     1261 tatcgccatg gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga
     1321 cccattggta ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat
     1381 tgtcggtccg aaaatgatca cccgcgtaca ggccgttgct accgtgctgg cgctgatccc
     1441 catcgtcggg attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc
     1501 atggaacgtc agcggtctgg gcaccttcgg tgcgattcaa agtaccctta acgttacgct
     1561 gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa
     1621 acgcaacgtc cctatcgcca ccattggcgg ggtattgatt gctgccgttt gctatgtact
     1681 ttctaccacc gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcttcgcc
     1741 attcggtgat gccgcacgga tggcgttggg tgacaccgcc ggggccattg tttccttctg
     1801 cgcagctgcg ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc
     1861 gaaagccgct gccgatgacg gactgttccc accgattttt gcccgtgtaa ataaagcggg
     1921 cacgccagtg gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagtag
     1981 catttcgcca aacgcgacta aagaattcgg tctggtttct tccgtgtcgg tcatctttac
     2041 actggtgcca tatctttaca cctgtgcggc gttactactg ctcggccacg gtcactttgg
     2101 taaagcacgc ccggcatacc tggcagttac taccattgcc ttcctctact gcatctgggc
     2161 cgtagtaggg tccggcgcga aagaggttat gtggtcattt gtcactctga tggtaatcac
     2221 cgccatgtat gcgctgaatt acaaccggct gcataaaaac ccgtatccct tagatgcacc
     2281 aataagcaaa gattaattcc ccttaatcaa gcaaacataa aagccaacct taagaactta
     2341 aggttggctt aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga
     2401 tgttgaagcg cctactaaaa agaccctctt taaatttatt ctcctggtta ttacttgcta
     2461 ctttttatat ctcagtctgc ctgaatatcg ccttttttaa acaggtgcta caggtgttac
     2521 cgctggattc actacataac gtgctggttt tcttgtcgat gccggtcgtc gcattcagcg
     2581 tgattaatat tgtcctgaca ctaggctctt tcttatggct taatcgtcca ctcgcctgcc
     2641 tgtttattct ggtcggtgcg gctgcacaat atttcatcat gacctatggc atcgtcatcg
     2701 accgttcaat gatcaccaat atcattgata ctacaccggc ggaaagttat gcgttgatga
     2761 cgccacgaat gttattaacg ctggggttaa gtggcgtgtt agccgcgatt attgcctgct
     2821 ggatcaaaat caaacccacc acctcacgcc tgcgcagcgt tcttttccgc ggggccaaca
     2881 tcctgatttc tgtgctgctg attctgctgg ttgccgcact gttctacaaa gactatgcct
     2941 cgctgtttcg caacaacaaa gagctggtga aatccttaag cccgtccaac agcattgttg
     3001 ctagctggtc gtggtactct caccagcggt cagccaacct gccactgata cgcattggtg
     3061 aagatgctca ccgcaaccca ttaatgcaaa atgggaaacg caaaaacctg accattttga
     3121 tcgtcggaga aacgtcgcgc gcagagaatt tctcgctgaa tggttatcca cgcgaaacca
     3181 atcctcgcct ggcaaaagat aacgtggtct atttccctaa taccgcatct tgtggcacag
     3241 caacagccgt ctcagtgccg tgcatgttct cggatatgcc gcgtgagcac tacaaagaag
     3301 agctggcaca gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc
     3361 tgtggaatga caacgatggc ggctgtaaag gcgtttgcga tcgcgtacct caccagaacg
     3421 tcaccgcgct gaacctgcct ggtcagtgca tcaacggcga atgctatgat gaagtgctgt
     3481 tccacgggct ggaagattac atcaataacc tgcagggtga tggcgtgatt gtcttacaca
     3541 ccatcggcag ccacggcccg acctattaca accgctatcc gccgcagttc aggaaattta
     3601 ccccaacctg cgacactaac gagatccaga cctgtaccca agagcaactg gtgaacactt
     3661 acgacaacac gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag
     3721 aacatcagga taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag
     3781 gtgaaaatgg catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac
     3841 aggtgccgat gctgctgtgg ctatcggagg attatcaaaa acggtatcag gttgaccaga
     3901 actgcctgca aaaacaggcg caaacgcaac actattcaca agacaattta ttctcaacct
     3961 tattgggcct gactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa
     4021 cttgcaggag agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca
     4081 gggattgatt ctggcggcgc aaaccgaagg ctacgcgtgc gatggcgtga gcaccgcgcg
     4141 gatggcggaa caaagccttg aggccggtca ttacagcctg gtggtactgg atttaggctt
     4201 acccgatgaa gatgggctgc attttcttgc ccgtatccgg cagaaaaaat acactctgcc
     4261 ggtgttaatt ctcaccgccc gcgataccct taccgacaaa atcgccgggc tggatgtcgg
     4321 ggccgacgat tatctggtga agccttttgc gctggaagag ttgcacgccc gtatccgcgc
     4381 cctgctacga cgccataata atcagggcga aagtgagctg aacgttggca acctgacgct
     4441 gaatatgggt cgccgccagg tatggatgag cggagaagaa ctgattctga cgcccaaaga
     4501 atatgctctg ctgtcgcgcc tgatgcttaa agcaggcagc ccggtgcatc gggaaattct
     4561 ctacaacgat atctacaact gggacaacga gccctcgacc aacaccctgg aagtgcatat
     4621 ccataacctg cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gatttggcta
     4681 tatgctggtc gcaaatgagg aaaactaatt gaatctgatg cgttttctgc gccgaccaat
     4741 atcgctgcgc caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat
     4801 cagcgtcttc tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct
     4861 acgcgacaat cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag
     4921 cctgattgtc cccggcgtct ttatggttag cctgacgcta tttatctgct atcaggcggt
     4981 acgccgcatc acccgaccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga
     5041 caacttaacg cccattgcca ttcatagcgc caccctcgaa atcgatgcgg tggtttcggc
     5101 attaaacgat ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc
     5161 tgacgtcgcg cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct
     5221 ggcgaaaacg catcacattg atgtagcacc gttagtggca cgacttgatc agatgatgga
     5281 gagcgtctcc cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta
     5341 tcaacatgta aaactgctgg aagatgtcat tctcccctcg tatgacgaac ttagtaccat
     5401 gctcgaccag cgccagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca
     5461 gggtgatgca accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta
     5521 tagcccacaa ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc
     5581 cgttgaagat gaaggaccgg gtattgatga gagtaaatgc ggggagttga gtaaagcgtt
     5641 tgtacgaatg gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat
     5701 tacacagttg catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg
     5761 ggcctggata cggctgaaaa aagatcagta cgtggcaaac cagatataaa gaaagctgct
     5821 gaccaccagc acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact
     5881 ctcctccgtt aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc
     5941 aattgacctg attagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg
     6001 cggatgttgc tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat
     6061 atcatcgatt ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg
     6121 tatatctgac gccgctggtg tcgcaccttt caacggtcga ttcgccgtct ctttcatggt
     6181 tacgccggtg attaaaccaa ccaccgccac taccatcagg taataggcag gcatcatcag
     6241 attctgcgag ctttcgacca gccaggccgc cagcgttggc gtcagaccgg caaccagcac
     6301 cgaaatatta aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgcagg
     6361 caaggtagaa gccataacgc ccgtaaagca gttaaggatc accgccagca tcaataagcc
     6421 ggcaaaaatc aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac
     6481 aaacagtgca acactaccaa gaagcacaaa cggacgacgg ccaaaacggt cactcagcaa
     6541 gcccatcacc ggttgaacaa acagcatacc gatcataatg gcgataataa tcagcacccc
     6601 gtggtcttcg gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat
     6661 gtagtaagtc acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagtg
     6721 tttggtggca atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc
     6781 ctgttccagt ttatcgacat gctgctggaa cgccggagtc tcttccagcg catggcgcag
     6841 gtaaagcccg ataatcccta acggcagagc gataaagaac ggaatacgcc agccccaatc
     6901 gaggaagttc tcttcgccga caatggtcga aattaacacc actacgccag cacccagcac
     6961 aaacccggca atagaaccaa agtccagcca gctgcccata aagccacgtt tacggtcagg
     7021 ggagtattcc gcaacaaata ttgacgcccc ggtatattca ccgccgaccg agaaaccttg
     7081 tgccatctta cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg
     7141 tattaagcca atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt
     7201 ctggcgacca tatttatcgc ccaacatacc aaagaagagc ccgccaagcg gtcgaatcag
     7261 aaagggaacg gagaaagtgg cgagtgcagc aaccatctgc acgctggggt cagcccccgg
     7321 gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa acaccaaaat cgaaccattc
     7381 cattgcatta cccagtgatg ctgcggtaat ggctttacgc agtttaccat catcaataat
     7441 ggtgacatca cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc
     7501 agagttggcc catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc
     7561 gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ctctccggtt
     7621 aaggaatgta caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc
     7681 taccagtgtt taaatttgtg atcaacttca cacatgtatt tcgtttagga ctcattggtg
     7741 taactgatta tttacaccaa atttacagag aagatttcct cgaccattgc ccccgccaga
     7801 cgctgcgctg aacagagtcg tggcatcccc agtgcgacag ttcgccagcg ttgggtcact
     7861 gaccagcaaa cgggttgttt atctgccgta gaccactttc ccagccaact taaaaaatca
     7921 gcatgatccc cgcccgtcga aacggtcgaa gaggcaaacc actgatggta atagtgacgc
     7981 gtggtcgggg ccgcattgat actctctgcc aaatgttgtg tcgctttcac gcgcaaatca
     8041 cccagcaaca acgccaccgc ttgcgggctt cccagacggc aggcatcgcc aaatgcaccc
     8101 cagcgcaact cctccagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc
     8161 ccagcacgcc agcgggtaaa aatttgctcg ctctgttcac ccgcctgcac caataggcct
     8221 cgttgcgtta acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg
     8281 acaatttgct gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc
     8341 agcgccagat gctgcgcgat ctgcgtcagc gccagatgac cgggatccag catcgacgcc
     8401 agtttctcca ccaatgtcag acgcagacta agcggcgcat ttaacatgcc gccgttcgtc
     8461 catgtctgaa gctgcacgcg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc
     8521 tgatgggctt gtggaaggcg ggcatcatcg ccctgcgcca gatctactaa aaagcgcgga
     8581 tgaaccgact ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg
     8641 ccgtgaaaag tgtttgaata tcatcgcgta acagctgagc gtcggtgtaa atccagccgt
     8701 gagtcatcac agtctgctgc aattgctgtt gcatcagcct gaccaccgat tcattttgtt
     8761 gacgcagagc catactttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact
     8821 cagcgaaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt
     8881 tagccatttg ttggcaataa agatcgactt ctgcgcttaa tgctcgttgc aacacactgt
     8941 aatcaaccgt ctctgtcgga gatttctcat ttccccgtcc ccagtcgggc tgattcaacc
     9001 agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcttcct
     9061 ggcgcgaaat gacttcactg aacagctggc gggtgttgaa gtggggaact acgccgtgaa
     9121 aaacaggaaa atgaaaccca ggacgaaacc cagactcact caattccatt ttgacttgtt
     9181 gcgcaatggg gcgaataaca tcggttaaca cccggcaaag ggtggactca agctcggcaa
     9241 agcgcagcgt aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc
     9301 gggtacgcat ctcacttaac gactccgaat tatcctgaaa caacgcggct aactgcgcat
     9361 tcaccgcatc ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc aactcgattt
     9421 catgtttaat ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt
     9481 cgacctgatg gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa
     9541 aatccaggta ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgcg gcagatcgca
     9601 gagcatacaa cgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga
     9661 acagcgaatc ttcccacaac tgatcggcag catggcgaat atgttcgagg tccgccagat
     9721 cggcatgacg ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt
     9781 gctctggcgc gggtaactta ccgttgttgg ctaactcatg gcgcgcccga ttcgccaggt
     9841 agccccacat cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg
     9901 ttccggaaat cagtgcccgc acctggtcag cgtcgtcact gttacgatcc tgttgatcga
     9961 acttattgac cagcacatac aacggcaccg attgccccac cgccaaaatt gcctcacgga
    10021 cctcttcatc ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg
    10081 cgcgtgccag ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc
    10141 cgggggtatc cagtaacgtc aactgaccgg gataactctc cagccccgcc agatggacaa
    10201 actccacttc aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga
    10261 cgtccagcgc cttcgccagt cgcaccaaat cattcaaact tttcagacaa tgaaaaatag
    10321 gttgagcacc cagataatat ttttcgaaag cgatgccatt ttcgatccgc tgcataagcg
    10381 cacgcatatc tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca
    10441 ggcgctgttg taattgttga attaaatggt cgattggcgc gacatgtgaa aaatgcagta
    10501 ccggttcttt ttgcccgggc gtatggcgaa taagcgtcgg cagctcagtc attgggcgat
    10561 tgcgattagg cagaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt
    10621 tcatagtacc gacaatcgcc agcaccattt ccagtcggga aattttacgc aactcattat
    10681 tcagcatcgc gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg
    10741 catcgtctcc gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa
    10801 gctgtaacag gcgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa
    10861 atttttcctt
//
LOCUS       DAZFRG010000163        10854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFRG010000163 DAZFRG010000000
VERSION     DAZFRG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.163"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..156
                     /locus_tag="ACHKUC_003406"
     CDS             <1..156
                     /locus_tag="ACHKUC_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO6619609.1"
                     /translation="PFCTGKYRWHGSTEAYTGREVQDIPGVLAVFAERRKDSFGPYVR
                     LMSVTLN"
     gene            235..612
                     /gene="cbeA"
                     /locus_tag="ACHKUC_003407"
     CDS             235..612
                     /gene="cbeA"
                     /locus_tag="ACHKUC_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO6619610.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPAITV"
     gene            659..1036
                     /locus_tag="ACHKUC_003408"
     CDS             659..1036
                     /locus_tag="ACHKUC_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6619611.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITRGKHPEAKQ"
     gene            1033..1182
                     /locus_tag="ACHKUC_003409"
                     /pseudo
     CDS             1033..1182
                     /locus_tag="ACHKUC_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            1258..1455
                     /locus_tag="ACHKUC_003410"
     CDS             1258..1455
                     /locus_tag="ACHKUC_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6619612.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            1540..2388
                     /locus_tag="ACHKUC_003411"
     CDS             1540..2388
                     /locus_tag="ACHKUC_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6619613.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEHDNFPEISEANILSTFEQLHQNKDEVFERGVINIFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLDDLERMLHLFSDKPIPENR
                     ENITLRLDDHIRSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYAG
                     KVSSSV"
     gene            2544..4043
                     /gene="istA"
                     /locus_tag="ACHKUC_003412"
     CDS             2544..4043
                     /gene="istA"
                     /locus_tag="ACHKUC_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family transposase"
                     /protein_id="HFO6619614.1"
                     /translation="MTLNTSQVSYYMTQRKKGITQHISAMKAGISVRSGRRIEKGEWA
                     KNSVRHWRTRKDPLEAVWDSMLVPLLKERPALTPTTLLEMLQDKYPGQYPNSLRRTMQ
                     RRVCEWKLQYGAEQEVMFRQRHQPGLRGLSDFTELKGVVVTIAGKLLAHKLYHFRLEW
                     SHWSWMRVVLGGESFSALAEGLQEALGQLGGVPVEHKTDSLRAAWKQQGEDGRRELTE
                     RYAALCQHYGMQGVHNNAGRGHENGSVESAHGHLKRRICQALILRGSNDFSTIEEYQA
                     FITQQVMRHNRNNQDLVKEERLHLKPLPLRRSADYDELTVRVSRSSTINVKHVVYSVP
                     SRLVGQLLRVRLWDDRLSCYVGSSEVMSCPRVRPEKGKTRARRIDFRHVIDSLAKKPG
                     AFCHATLRNDILPDDEWRRLWRRLCNHLEPDMAGRLMVHALKLAAGYDDISVVAKGME
                     QMLNTPGNVDLHRLMRFLGIKEKALPVVNVKQHNLSSYEQLLRGKGGSQ"
     gene            4040..4795
                     /gene="istB"
                     /locus_tag="ACHKUC_003413"
     CDS             4040..4795
                     /gene="istB"
                     /locus_tag="ACHKUC_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
                     /protein_id="HFO6619615.1"
                     /translation="MSNIHHLERSLRKLRLTRVGAEWHALEKRALAEGWTPSRYLLTL
                     CNEELLWRESEKLRRYKKEARLPVAKTLSEYDFSQVPELNGAQFRQLCETTDWVDAGE
                     NVLLFGASGLGKSHLAAAIVDGVVGQGYRARFYSAGELLQELRKARAQLKLNELLLKL
                     DRYRVIVVDDLGYVKRDSAETGVLFELIAHRYERGSLVITSNHPFSMWGSIFVDETMA
                     VAAADRLIHHGYMFELKGESYRKKTAKAVTSAT"
     gene            5108..5767
                     /gene="glnP"
                     /locus_tag="ACHKUC_003414"
     CDS             5108..5767
                     /gene="glnP"
                     /locus_tag="ACHKUC_003414"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO6619616.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            5764..6486
                     /gene="glnQ"
                     /locus_tag="ACHKUC_003415"
     CDS             5764..6486
                     /gene="glnQ"
                     /locus_tag="ACHKUC_003415"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO6619617.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            6603..8828
                     /gene="ybiO"
                     /locus_tag="ACHKUC_003416"
     CDS             6603..8828
                     /gene="ybiO"
                     /locus_tag="ACHKUC_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO6619618.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(8825..9751)
                     /gene="rlmF"
                     /locus_tag="ACHKUC_003417"
     CDS             complement(8825..9751)
                     /gene="rlmF"
                     /locus_tag="ACHKUC_003417"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO6619619.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            10027..10287
                     /gene="mcbA"
                     /locus_tag="ACHKUC_003418"
     CDS             10027..10287
                     /gene="mcbA"
                     /locus_tag="ACHKUC_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO6619620.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            10552..>10854
                     /locus_tag="ACHKUC_003419"
     CDS             10552..>10854
                     /locus_tag="ACHKUC_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO6619621.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         2650 a         2775 c         2882 g         2547 t
ORIGIN      
        1 ccgttctgta ccggcaaata ccgctggcac ggcagcactg aagcgtatac cggtcgtgaa
       61 gtgcaggata ttcccggtgt gctggctgtg tttgcagaac gccgtaaaga cagttttggc
      121 ccgtatgtcc ggctgatgag cgtcaccctg aactgagtca ggaagggcat tctgatgagc
      181 cgaattatcg ccaccatcga accattctta actgatttct gtgaggactt tatcgtgtca
      241 gacacactgc ccgggacaac gcatcccgac gacaatcacg accgcccctg gtgggggctg
      301 ccctgcaccg tgacgccctg tttcggggca cgtctggtgc aggagggtaa ccggttgcat
      361 taccttgccg accgtgccgg tatcagaggc cggttcagcg acgcggatgc ataccacctg
      421 gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg
      481 aatccccgcc atcagcatac catcacactg tatgcgaaag ggctgacctg cgaagccgat
      541 acccttggca gttgtggtta cgtttatctg gctgtttatc cgacaccggc agcgcccgca
      601 atcaccgtat aaccccggat atctcccctt tatcaccacc tgttacagag aacttaagat
      661 gaatacatta cccgacactc acgtacggga ggcatcgggc tgcccgtctc ccgtcaccat
      721 ctggcagaca ctgctcaccc gactgctgga ccagcactat ggcctcacgc tgaatgacac
      781 accgttcgct gatgaacgtg tgattgagca gcatattgag gcagggattt cactgtgtga
      841 cgcggtgaac tttctcgttg aaaaatacgc gctggtacgt accgaccagc cgggattcag
      901 cgcaggagcc ccgtcgcagt taatcaacag cattgatatt ctccgggctc gcagggcaac
      961 cggcctgatg acccggaaca attacagaat ggtaaataac attacccggg gcaagcatcc
     1021 ggaggcaaaa caatgaaact ggccctgacg ctggaagccg acagcgttaa cgtgcaggca
     1081 ctgaacatgg ggcgcattgt cgttgacgtc gatggtattg agctcgctga actgattaac
     1141 aaaatcgctg aaaacggtta ttcactccgc gtggtggatg aacgtgatgc taccgaaaca
     1201 ccggcgactt ctgccagccc tcatcagttg ccggaagaca atacagtgat acagagcgtg
     1261 atgccattaa ccactgaaac agcgctggat atcctcattg cgtggctgca ggacaatatc
     1321 gactgcgaat cgggaattat ctttgacaac gatgaggata aaacggattc ggcagcactg
     1381 ttgccctgta tcgaacaggc ccgggaggat gtccgtactc ttcgtcatct gcagcttctg
     1441 caccagaacc ggtaaatatc actcaccata cttcattcca ctgacgtcag cctgaacagg
     1501 gctggcgttt tcgtttataa accaaaaggg aaaccgataa tgtcagacat cacaatctcc
     1561 cgtccggaag tggtcaccgg gcatacggac gttatctgct caaccagtat ccgccacatt
     1621 ctggctgtac gaaagagtac actgctgcaa atcgacacac ttatccggca actggctgaa
     1681 atatcagcaa tgacagaaag tattggtggt aaagccgccc cggactgggc catgaaacag
     1741 gattttcgct gcggctgctg gctgatggag aaacctgaaa cggcaatgaa agccatcaca
     1801 cgcaatctcg atcgcgaaat ctggcgtgac ctgatgcaac gttcagggat gctttcctta
     1861 atggatgcac aggcccgtga tacatggtac cggtcactgg agcacgataa ttttccggaa
     1921 atcagtgaag cgaacattct gagcacattt gaacaactac accagaataa ggatgaggtg
     1981 tttgagcgag gggtgatcaa catcttcagg ggacttagct ggaattacaa aactaattca
     2041 ccctgtaaat ttggcagtaa aattatcgtc aacaacctgg tgaggtggga ccgatggggg
     2101 tttcatctta tcaccgggca acaggcagat cgacttgacg atctggaaag aatgctgcac
     2161 ctgttcagcg ataaaccaat ccccgaaaac cgggaaaaca tcacccttcg tctggatgat
     2221 cacatccggt ctgttcaggg taaagaggac tatgaagatg agatgttcag catcagatac
     2281 tttaagaagg gctccgcaca catcacgttc aggaaaccag aactggttga caggctgaat
     2341 gacatcattg cgaaacatta tgcgggaaaa gtctcctctt cagtataaac ttacacgcat
     2401 cacagtcctg cgcccggaac aagactggca ggtatattgc attcgctgag tatgagacga
     2461 caataactca gtccgcctgg cgccgattat tctggccggt tttgtaacct gagtgtcatt
     2521 tacatgatcc aacaggaaat ggcgtgacgc tcaatacttc tcaggtcagt tactatatga
     2581 ctcagcgtaa gaaaggtata actcagcata tctcggccat gaaggctggt atctcagtcc
     2641 gttctggtcg tcggatcgaa aaaggagagt gggcaaaaaa cagtgttcgg cactggcgca
     2701 cacgcaaaga tcctctggaa gctgtgtggg acagcatgct tgttcctctg ttgaaagaga
     2761 ggccggctct gacaccaaca actctgctgg agatgctaca ggataaatat cccggccagt
     2821 accccaacag ccttcgaaga acaatgcaac ggcgggtctg cgaatggaag ctacagtatg
     2881 gtgcagagca ggaggtcatg ttccgccagc gacatcagcc cggtctgcga ggtctgtcgg
     2941 actttactga actgaaaggt gtagttgtca ccatcgccgg taagttgttg gcgcataagt
     3001 tgtatcactt ccgtctggaa tggagccact ggagctggat gcgggttgtg ctgggtggtg
     3061 agagcttctc tgctctggct gaaggtctgc aggaagccct cggacaactg ggcggagtgc
     3121 cggtagaaca taaaacggac agcctgaggg cagcatggaa acaacagggc gaagatggac
     3181 gccgcgagct gactgagcgt tatgctgctc tctgtcagca ctacggaatg cagggcgtac
     3241 acaataatgc cggtcggggc cacgaaaatg gctcggttga aagtgcccac ggacatctga
     3301 aaaggcgtat ctgtcaggcg ctgatactgc ggggcagtaa cgacttcagc actatagaag
     3361 aatatcaggc cttcatcact cagcaggtta tgcggcacaa ccgtaacaat caggatctgg
     3421 tcaaggaaga acgtcttcat ctgaaaccgc tgccgcttcg tcgcagtgct gactatgatg
     3481 agctgactgt gagggttagc cgcagcagta ccatcaatgt gaagcacgtc gtctacagcg
     3541 taccttcccg gcttgtaggt caactgttac gggtccggtt atgggacgat cgtctgagct
     3601 gttacgttgg cagcagcgag gtcatgagct gcccacgtgt cagaccagaa aaagggaaga
     3661 cgcgggcccg tcgtatcgac ttccgacatg tgatcgacag tctggcaaaa aagcccggtg
     3721 cgttctgcca tgcaacgctg agaaatgaca tcctgccaga cgatgaatgg cggaggctgt
     3781 ggcgtcgctt atgtaatcat ctggaacccg acatggcagg caggctgatg gtacatgctc
     3841 tgaaactggc tgcaggatac gacgatatct cagtcgtggc aaaaggtatg gagcagatgc
     3901 tgaatacccc gggaaacgtg gatctgcacc ggctgatgcg cttcctgggt ataaaggaaa
     3961 aggcgttgcc ggtagtcaat gtgaaacagc ataacctgag cagttatgag caactactgc
     4021 gtggcaaggg aggttcgcag tgagcaatat ccatcacctt gaacgcagcc tgcgtaaact
     4081 acgcctgaca cgagttggag ctgaatggca cgctctggaa aaacgagcac tggcagaagg
     4141 ctggacacca tcgcgctatc ttctgacgct atgcaatgaa gaactcctgt ggcgcgagag
     4201 tgaaaaactg cgtcgttata aaaaggaggc ccggttgcca gttgccaaaa cgctaagcga
     4261 atacgacttc agtcaggtgc cggaactgaa tggagctcag ttccggcaac tctgtgaaac
     4321 gacagactgg gttgatgcag gagaaaacgt tctgctgttc ggagccagcg ggttggggaa
     4381 aagccatctg gcggcagcga tcgtggatgg cgtagtaggc cagggctacc gggcccggtt
     4441 ctacagcgca ggagagttgt tgcaggaact acgtaaagcc agagcgcagt tgaaactgaa
     4501 tgagctgcta ctgaaactgg atcgctaccg ggtgatagtg gtggatgatc ttggctatgt
     4561 caaacgcgac agcgccgaaa cgggagtact gttcgagtta atagcgcatc gctatgaacg
     4621 tgggagcctg gtgataacca gtaaccatcc gttcagcatg tggggcagca tcttcgtgga
     4681 tgagactatg gcggtggcgg cggcagaccg gctgatccat cacggatata tgttcgaact
     4741 gaaaggtgaa agctacagga aaaagacagc gaaggcagta acaagcgcga cttgatgtcg
     4801 cactgaaggg tgcggccagt atagttggcg cgagtcggca aaactagttg acgtctaata
     4861 gctgagccat taatgataat gcctcaactg agttcaatac acctccatgt acaacaacag
     4921 tactttgggg gccaaattgg gggctacatt tttacaacaa aagaaataaa ccacaattaa
     4981 tcaacgagat aaacatattt cactattccc cgccggggag aggattaggg tgaagggcgc
     5041 aaacccgctc cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga
     5101 acaacatatg cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg
     5161 tgccaaaatg accctgtgga tttcggtcct cggtctggct ggcggtctgg tgatcggatt
     5221 gttggcaggt tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt
     5281 tattgaagtg atccgcggca cacctatcgt cgtccaggtg atgtttatct atttcgccct
     5341 gccgatggcg tttaacgatt tacgcatcga cccgtttact gcggcggtgg tcaccatcat
     5401 gatcaactcc ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa
     5461 aggttttcgt gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt
     5521 cattttaccg ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag
     5581 cattaaagac acttcgctgt ttattgttat cggcgtggcg gaactgaccc gtcaggggca
     5641 agaaattatt gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta
     5701 tctgattatt accctggtgc tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat
     5761 cctgtgattg aatttaaaaa cgtctccaag cattttggtc caacccaggt gctgcacaat
     5821 atcgatttga acattgctca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt
     5881 aaatcgaccc tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt
     5941 gtcgatggcc tgaaggttaa cgatccgaaa gttgatgagc gcctgattcg ccaggaagcg
     6001 ggtatggtgt tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg
     6061 tttggcccgc tacgcgtgcg tggcgcgaat aaagaagagg cggaaaaact ggcgcgtgag
     6121 ctgctggcga aagtcggtct ggcagagcgt gcacatcact acccttccga actttctggt
     6181 ggtcaacagc agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg
     6241 tttgatgaac cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg
     6301 caggatctgg ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc
     6361 gagaaagtag cttcgcgcct gatctttatc gacaaaggcc ggattgcaga agatggcgat
     6421 ccgcaggtgt tgatcaagaa cccgccgagc cagcgcttgc aggaattttt gcagcacgtc
     6481 tcttaacttc tctgcctccc tttcccccga aagggaggca caccagattc ctctcattta
     6541 aaatcgcctc tcccccagca tctatactta tctttttgct ctgttttctc actggaggag
     6601 tcatgcggtg gatcctgttc atcctcttct gcctgttggg cgcacctgcc cacgcggtat
     6661 ccatacccgg cgttacaacc acaacgacaa cggactcaac gactgaaccg gccccggaac
     6721 cggatatcga acaaaaaaaa gcggcctatg gcgcactggc ggatgtgctg gataatgaca
     6781 cctcgcgtaa agagttgatc gaccagttgc gcaccgtcgc cgctacgcct cctgcggaac
     6841 ccgtaccgaa gatcgtgccg ccgacgctgg ttgaagagca aaccgtgctg caaaaagtca
     6901 ccgaagtcag ccgccattat ggtgaagccc tttccgcccg cttcgggcaa ctttatcgca
     6961 atatcaccgg ctccccgcat aagccgttta atccacaaac cttcagcaat gcgctgaccc
     7021 acttttcaat gttagcggta ttagtgtttg gtttttactg gctgattcgt ctgtgcgcac
     7081 tgccgctgta tcgcaaaatg ggccagtggg cgcggcaaaa aaatcgtgag cgcagtaact
     7141 ggttgcagct tccggcgatg atcatcgggg cgtttattat cgacctgctg ttactggcac
     7201 tgacattatt tgtcggccag gtattaagcg acaacctgaa tgcgggcagt cgcactatcg
     7261 ctttccaaca aagtttgttt ctcaacgcct ttgccctgat tgaatttttc aaagccgtac
     7321 tacgcctgat tttttgccca aacgtagctg aactgcgccc gttcacgatt catgacgaga
     7381 ccgcccgtta ctggagtcgt cgcctgagct ggttaagcag cctgattggc tatggtctga
     7441 ttgtggctgt gccgattatc tctaatcagg tgaatgtaca gataggtgcg ctggcgaacg
     7501 tcatcattat gctgtgcatg accgtctggg cgttgtacct gatttttcgt aataaaaaag
     7561 agatcaccca gcatttgctc aactttgcgg agcattcgct ggcctttttc agcctgttta
     7621 tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc gtgctgtttt
     7681 tcttttcgtt gttcgatccg ggcaacagcc tgaaatttat gatgggtgca acggtgcgca
     7741 gcctggcgat tattggtatc gcagcatttg tttccggtat gttttcccgc tggctggcga
     7801 aaaccatcac cctctcgcca catactcagc gaaattatcc ggagctgcaa aaacggctga
     7861 atggctggct ttcggcggca ctgaaaacag cgcgtattct gacagtctgc gtggcggtaa
     7921 tgctgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa aacggcgcgg
     7981 ggcagaaaac cgtagatatc ctgatccgta tcgcactcat tcttttcttc tcggcggttg
     8041 gctggacggt gctcgccagt ttgatagaaa accggctggc ttcggatatt catggccgcc
     8101 cgctacccag cgcccgtacg cgaaccctgc tgacgctgtt ccgtaacgcg ctggcggtga
     8161 ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgtcaat atcgcgccat
     8221 tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa acgctggtga
     8281 aagatattat taccggagta tttattcagt ttgaaaacgg catgaacact ggagatttgg
     8341 tgactatcgg gccgttgacc ggcactgtgg aacggatgtc gattcgctcc gtgggcgtgc
     8401 gccaggatac cggggcgtat cacatcattc cgtggtcttc gataaccacc tttgctaact
     8461 tcgtccgcgg tattggttcg gtagtggcaa actacgatgt tgatcgccat gaagatgctg
     8521 ataaagccaa tcaggcactg aaagatgcgg tagcagaatt gatggaaaac gaagaaattc
     8581 gcgggctggt tattggtgaa ccgaactttg ccgggattgt cggcttaagc aataccgcgt
     8641 ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggtc cgctttgccc
     8701 tcgacagcca ggtgaaaaaa catttcgacc tggcgggtgt tcgcgcgccg gtgcagactt
     8761 atcaggtgct gccagctccg ggcgcggccc cggctgaacc gttgccgccg ggggagccaa
     8821 cgctttaacg ctggcgattg acaaaacggc ggcgctgctc gtcgttcata aaggtccagg
     8881 ctataaagcg gctctgcttt tgcccctggg ccatctcttt ttttaccacc ttcaccgcac
     8941 ctacgtccgt cagagcacga tacaacggcg gtaagttttc accacgagat accagcgagg
     9001 taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt ttgataaaag
     9061 cgacctcacc gccttcacac cacaactcct gttgctgacc accaaagttc agtgcatcgt
     9121 ctttgttcag ccccaggtta cggcgtttac gttcactacc tgcccgtgcc gcagcggcgg
     9181 aatcgtggaa tggcgggtta cacaaggtcg catcgtattg ctcgttttta tggatgatgc
     9241 cgttaaaaat cgccccactc tctttttgcc gacgcagacg aatagcgcgg ttaagccccg
     9301 gattagcact gatgatcgcc tgcgcactgc ttaacgcctg gctgctggtt tcactaccgg
     9361 taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc gcaccaacgc
     9421 cgatatccag aatgctggca ttagctggaa ttgttccgct tgcttcagcc agtaaatcgg
     9481 caaggtgatg aatataatcc gcccggcccg gtactggcgg gcagagaaaa ccgtccggga
     9541 tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc gccttcaccg
     9601 ccagcggatt ggcaaagtct acgctttgct ccccggcggg tgtaagcgtg aggaattgcc
     9661 tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga tgacggttgc
     9721 gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag cggcgtaaga
     9781 tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcatttcta aaaaccttca
     9841 catggctatg gtttatggca attcaccata aaaatcatca tcatgcatgt cattgatttg
     9901 tcttattttg taaaaacata tcgttcaaaa aataagcgca tctctgtcaa ttttttgcgt
     9961 cattttcatc gcgcaggcga cctacactta tctggcacta cccgttgtat ttatgaggat
    10021 gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcgg aatctctctc
    10081 acggcttatg ccgcgcaacc gatgagtaac ctggacagtg gtcaactacg gcccgccggt
    10141 accgtttcgg caactggcgc atcaaaccta agcgatctgg aggataaact ggcagaaaaa
    10201 gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg caggcggaaa tgaccagatg
    10261 ttcggtactg caatcatcta caaataaccc cacaaacgta actgcattcg cctctggatg
    10321 cagttttcgt ccaatttgta agcaaaaaac atcaaatatg aacgtaatgt aaataaatgt
    10381 atttcttttt cgcgcaatgg gtgatagaaa atcgcgccaa gtgataatgc ttatcaaaat
    10441 tattatcact tgcacggacg ctatcacggg attaacagtg gcatcgcatc cgcagagatg
    10501 ctttctcgtg acagtgaaaa tttcaccata taagaaaaag tcacctgcaa aatggaaaac
    10561 aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg tctttgtatt
    10621 ggtattacgc ctgtggcgca ggcactcgct gccgaagggc aagctaacgc ggatgacacg
    10681 ctggttgtcg aagcatcaac gccttcgctt tatgcgccac aacaatctgc cgatccgaaa
    10741 ttctcgcgtc cggtagcgga tactacccgc acgatgacag tgatttctga acaagtgatt
    10801 aaagatcagg gtgcaaccaa ccttaccgac gcgctcaaaa acgtccccgg cgtg
//
LOCUS       DAZFRG010000164        10803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFRG010000164 DAZFRG010000000
VERSION     DAZFRG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.164"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            493..2421
                     /gene="thrS"
                     /locus_tag="ACHKUC_003420"
     CDS             493..2421
                     /gene="thrS"
                     /locus_tag="ACHKUC_003420"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO6619622.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2425..2967
                     /gene="infC"
                     /locus_tag="ACHKUC_003421"
     CDS             2425..2967
                     /gene="infC"
                     /locus_tag="ACHKUC_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO6619623.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3064..3261
                     /gene="rpmI"
                     /locus_tag="ACHKUC_003422"
     CDS             3064..3261
                     /gene="rpmI"
                     /locus_tag="ACHKUC_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO6619624.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3314..3670
                     /gene="rplT"
                     /locus_tag="ACHKUC_003423"
     CDS             3314..3670
                     /gene="rplT"
                     /locus_tag="ACHKUC_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO6619625.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3787..3911
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3793..3837
                     /gene="pheM"
                     /locus_tag="ACHKUC_003424"
     CDS             3793..3837
                     /gene="pheM"
                     /locus_tag="ACHKUC_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO6619626.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4121..5104
                     /gene="pheS"
                     /locus_tag="ACHKUC_003425"
     CDS             4121..5104
                     /gene="pheS"
                     /locus_tag="ACHKUC_003425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO6619627.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAKQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5119..7506
                     /gene="pheT"
                     /locus_tag="ACHKUC_003426"
     CDS             5119..7506
                     /gene="pheT"
                     /locus_tag="ACHKUC_003426"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO6619628.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLDADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7511..7810
                     /gene="ihfA"
                     /locus_tag="ACHKUC_003427"
     CDS             7511..7810
                     /gene="ihfA"
                     /locus_tag="ACHKUC_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO6619629.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7911..8891
                     /gene="btuC"
                     /locus_tag="ACHKUC_003428"
     CDS             7911..8891
                     /gene="btuC"
                     /locus_tag="ACHKUC_003428"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO6619630.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8953..9504
                     /gene="btuE"
                     /locus_tag="ACHKUC_003429"
     CDS             8953..9504
                     /gene="btuE"
                     /locus_tag="ACHKUC_003429"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO6619631.1"
                     /translation="MQDSILTTVVKDIDGKVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9504..10253
                     /gene="btuD"
                     /locus_tag="ACHKUC_003430"
     CDS             9504..10253
                     /gene="btuD"
                     /locus_tag="ACHKUC_003430"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO6619632.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10331..10795
                     /gene="nlpC"
                     /locus_tag="ACHKUC_003431"
     CDS             10331..10795
                     /gene="nlpC"
                     /locus_tag="ACHKUC_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6619633.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         2699 a         2552 c         2938 g         2614 t
ORIGIN      
        1 tttgaggatg atgattgatt attatcgcca gaacgaaatg cgcacgaaaa attgctataa
       61 gaattatgtc acttttatca ttgcgatatg actacttcgt cattaatctc atgatcgttt
      121 aaatactacc gccaaatcac gcaacgaact ttgctggatt cagtttatgc tgctgtaaat
      181 ccgctcgtag taaacctttc agacactctg tgatgttatc agtggttctt taagcgtttt
      241 gctggtgtac tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa
      301 tctccctttt tttagtgtta acatcgttca accgggtgga aggtgagagg ttaatgtctt
      361 tcatttgtgt acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg
      421 aactgttaat aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa
      481 taaggatata aaatgcctgt tataactctt cctgatggca gccaacgcca ttacgaccac
      541 gctgtaagcc ccatggatgt tgcgctggac attggtccag gtctggcgaa agcctgtatc
      601 gcagggcgcg ttaatggcga actggttgat gcttgcgatc tgattgaaaa cgacgcacaa
      661 ctgtcgatca ttaccgccaa agacgaagaa ggtctggaga tcattcgtca ctcctgtgcg
      721 cacctgttag ggcacgcgat taaacaactt tggccgcata ccaaaatggc aatcggcccg
      781 gttattgaca acggttttta ttacgacgtt gatcttgacc gcacgttaac ccaggaagat
      841 gtcgaagcac tcgagaagcg gatgcatgag cttgctgaga aaaactacga cgtcattaag
      901 aagaaagtca gctggcatga agcgcgtgaa actttcgcca accgtgggga gagctacaaa
      961 gtctccattc ttgacgaaaa catcgcccat gatgacaagc caggtctgta cttccatgaa
     1021 gaatatgtcg atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc
     1081 aaactaatga aaacggcagg ggcttactgg cgtggcgaca gcaataacaa aatgttgcaa
     1141 cgtatttacg gtacggcgtg ggcagacaaa aaagcgctta acgcttacct gcaacgcctg
     1201 gaagaagccg cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct gtaccatatg
     1261 caggaagaag cgccgggtat ggtattctgg cacaacgacg gctggaccat cttccgtgaa
     1321 ctggaagtgt ttgttcgttc taaactgaaa gagtaccagt atcaggaagt taaaggtccg
     1381 ttcatgatgg accgtgtcct gtgggaaaaa accggtcact gggacaacta caaagatgca
     1441 atgttcacca cgtcttctga gaaccgtgaa tactgcatta agccgatgaa ctgcccgggg
     1501 cacgtacaaa ttttcaacca ggggctgaag tcttatcgcg atctgccgct gcgtatggcc
     1561 gagtttggta gctgccaccg taacgagccg tcaggttcgc tgcatggcct gatgcgcgtg
     1621 cgtggtttta cccaggatga cgcgcatatt ttctgtactg aagaacaaat tcgcgatgaa
     1681 gttaacggat gtatccgttt agtctatgat atgtacagca cttttggctt cgagaagatc
     1741 gtcgtcaaac tctccactcg tcctgaaaaa cgtattggca gcgacgaaat gtgggatcgt
     1801 gctgaggcgg acctggcggt tgcgctggaa gaaaacaaca tcccgtttga atatcaactg
     1861 ggtgaaggcg ctttctacgg tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt
     1921 gcatggcagt gcggtacagt acagctggac ttctctttgc cttctcgtct gagcgcttct
     1981 tatgtaggcg aagacaatga acgtaaagta ccggtaatga ttcaccgcgc aattctgggg
     2041 tcgatggaac gtttcatcgg tatcctgacc gaagaatttg ctggtttctt cccgacttgg
     2101 cttgcgccag ttcaggttgt tatcatgaat attaccgatt ctcagtctga atacgttaac
     2161 gaactgacgc aaaaactatc aaatgcgggc attcgtgtta aagcagactt gagaaatgag
     2221 aagattggct ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat gctggtctgt
     2281 ggtgataaag aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg taaagacctg
     2341 ggaagcatgg acgtaaatga agtgatcgag aagctgcaac aagagattcg cagccgcagt
     2401 cttaaacaat tggaggaata aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct
     2461 aaccgtatca atggcgaaat tcgcgcccag gaagttcgct taacaggtct ggaaggcgag
     2521 cagcttggta ttgtgagtct gagagaagct ctggagaaag cagaagaagc cggagtagac
     2581 ttagtcgaga tcagccctaa cgccgagccg ccggtttgtc gtataatgga ttacggcaaa
     2641 ttcctctatg aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa agttatccag
     2701 gttaaggaaa ttaaattccg tcctggtaca gatgaaggcg actatcaggt aaaactccgc
     2761 agcctgattc gctttctcga agagggtgat aaagccaaaa tcacgctgcg tttccgcggt
     2821 cgtgagatgg cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa agacgatttg
     2881 caagaactgg cagtggtcga atccttccca acgaagatcg aaggccgcca gatgatcatg
     2941 gtgctcgctc ctaagaagaa acagtaaggc cttcaagtag cactgtctgt ggagcctttg
     3001 ggcttcacag gcattgttcg cctttgtttc gtttattaac aatgcgaagt ggaagttatt
     3061 aaaatgccaa aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa
     3121 ggtggtttta agcacaagca cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa
     3181 cgtaaacgtc acctgcgtcc gaaagccatg gtttccaaag gcgatctggg cctggtaatc
     3241 gcgtgcctgc cgtacgcata agccgttaac gtttttaact ttttaattag aatatagata
     3301 caggagagca catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa
     3361 aattttgaaa caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt
     3421 ccaggctgtt atcaaagctg gtcagtatgc ttaccgcgac cgtcgtcaac gtaagcgtca
     3481 gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta
     3541 cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc
     3601 tgatatcgca gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc
     3661 tctggcataa gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca
     3721 aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag
     3781 gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag cacctgactc
     3841 caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag
     3901 gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggc gtaattgtcg
     3961 cagccagtct ggacacggcc agcgcggaga aaccggagcg tacttaagta cgtgaggatt
     4021 tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggatg ggctctaagt
     4081 ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac atgtcacatc tcgcagaact
     4141 ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt
     4201 gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga
     4261 gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt
     4321 tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gccgcactga atgcgcgtct
     4381 ggcggcggaa acgattgatg tctccctgcc gggtcgtcgc attgaaaacg gtgggctgca
     4441 tccggtgacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt
     4501 ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg
     4561 tcaccacccg gcgcgcgctg accacgacac tttctggttt gacgctaccc gcctgctgcg
     4621 tacccagacc tctggcgtac agatccgcac catgaaagcc aagcagccac cgattcgtat
     4681 catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca
     4741 tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga aaggcacgct
     4801 gcacgacttc ctgcgtaact tctttgagga agatttgcag atccgcttcc gtccttccta
     4861 cttcccgttt accgaacctt ctgcagaagt ggacgtcatg gggaaaaacg gtaaatggct
     4921 ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc
     4981 ggaagtttac tctggttttg ccttcgggat ggggatggag cgtctgacca tgttgcgtta
     5041 cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa
     5101 ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg
     5161 gcgattgata gcgacgcgct ggcaaatcaa atcacgatgg cgggcctgga agttgatggt
     5221 gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg
     5281 cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg
     5341 ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagcgacc
     5401 attggtgctg ttctgccggg tgatttcaaa attaaagccg cgaaactgcg tggcgaaccg
     5461 tctgaaggga tgctgtgctc cttctctgag ctgggaattt ctgacgatca taacggcatt
     5521 atcgagctgc ctgcggatgc gccgattggc accgacatcc gcgaatacct gaaactcgat
     5581 gacaacacca tcgaaattag cgtgacgcca aaccgtgccg actgcttagg tatcattggt
     5641 gttgcgcgcg acgttgccgt gttgaaccag ctgccgctgg ttgaaccgga aatcgttccg
     5701 gttggtgcga ccatcgacga cacgctgccg attgcagtag aagcgccgga tgcctgcccg
     5761 cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg
     5821 aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac
     5881 tatgtgctgc tcgaactggg ccagccaatg cacgctttcg ataaagatcg cattgaaggc
     5941 ggcattgtgg tgcggatggc gaaagagggc gaaacgctgg tgttgctcga cggcactgaa
     6001 gcgaagctgg atgctgacac cctggtcatc gccgaccaca acaaggcgct ggcgatgggc
     6061 ggcatatttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt tctgctggaa
     6121 tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact
     6181 gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa
     6241 cgtgcgaccc gtctgctgat cgacatctgc ggtggtgaag cgggtccggt aattgatatc
     6301 actaacgaag caacgctgcc gaagcgtgca accatcactt tacgtcgtag caaactggat
     6361 cgcctgatcg gccatcatat tgctgatgag caggtaacag acattctgcg tcgtctcggc
     6421 tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg cgccgagctg gcgtttcgac
     6481 atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc
     6541 ccggatgagc cggtacaggc aagcctgatt atggggactc accgtgaagc tgacctgtcg
     6601 ctcaagcgcg taaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc
     6661 ttcgttgacc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca
     6721 agcccgatct ctgttgaaat gtcagcaatg cgtctttccc tgtggaccgg cctgctggca
     6781 accgtggtgt acaaccagaa ccgtcagcag aatcgtgtac gcattttcga aagcggtctg
     6841 cgtttcgtgc cagatactca ggcaccgttg ggtattcgtc aggatctgat gttagccggt
     6901 gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc
     6961 tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt
     7021 gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg
     7081 aaaggtgaac gtattggttt tgttggggtt gttcatcctg aactggaacg taaactggat
     7141 ctcaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg
     7201 cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc
     7261 gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag
     7321 gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag
     7381 agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt
     7441 gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg
     7501 gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg
     7561 gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc
     7621 tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa
     7681 gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg
     7741 cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa
     7801 agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct tttcactgtc
     7861 gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac
     7921 ttgcccgcca acaacagcga caaaatattc gctggttatt atgcttgtca gttttgatgc
     7981 tactggcgct tctcttaagc ctttgcgccg gtgaactatg gattttgcca ggtgactggt
     8041 tttctcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat
     8101 tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc
     8161 ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg
     8221 cggtattact tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg
     8281 gcgcgcttat catcacttta atactcttac gtttcgcccg tcgtcatctt tcgaccagtc
     8341 ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg acgtgggcta
     8401 tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg
     8461 gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatcccggtg ttgttgtgga
     8521 tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg gcgcggcaac
     8581 tgggtttacc cctgtggttc tggcgcaatg tgctggtggc agcgaccggc tggatggttg
     8641 gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc
     8701 tccggctgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg
     8761 cgagcgcatt gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc
     8821 caattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag
     8881 caggacgtta gccgcaaaac acggtctatg attaaaagct aagattttta ctgaccacac
     8941 ccaggagaaa cgatgcaaga ttccattctg acgaccgtag tgaaagatat tgacggtaaa
     9001 gtgaccacgc tggagaagta cgccggtaat gtgctgttga ttgtcaatgt cgcctcaaag
     9061 tgtggcttaa cgccgcaata tgagcagttg gagaatattc agaaagcctg ggctgatcgt
     9121 ggttttgtgg tgctgggatt cccgtgtaac cagtttctgg agcaagaacc gggtagcgat
     9181 gaagagatta aaacttattg caccaccaca tggggggtga cgttcccgat gttcagtaag
     9241 attgaagtta atggcgaagg acgtcatccg ctgtatcaaa aattgattgc cgcagcgccg
     9301 actgcagtcg cgccggaaga gagcggattc tatgcccgaa tggtcagcaa aggtcgtgca
     9361 ccgctgtacc ctgatgatat tttatggaat tttgagaaat ttttagttgg cagggacgga
     9421 ttggtcatcc agcgtttttc cccggatatg acgccggaag atcccattgt gatggaaagc
     9481 attaaactgg cgttggcaaa ataatgtcta ttgtgatgca gttacaggat gttgcggaat
     9541 ctacccgcct ggggccgctt tctggcgagg ttcgggctgg ggagatcctg cacctggtgg
     9601 ggccgaatgg cgcgggtaag agtaccttac tggcgcgaat ggccggaatg accagcggta
     9661 agggaagcat tcagttcgcg gggcaaccac tggaagcatg gtctgcaaca aagctcgcgc
     9721 tgcatcgcgc ctatctttca caacagcaga cgccgccgtt tgcaatgccg gtctggcact
     9781 acctgacact gcatcagcac gataaaacgc gtaccgaact actgaatgat gtcgcagggg
     9841 cgctggctct tgatgacaaa ctcggacgta gcaccaatca actttccggc ggtgaatggc
     9901 aacgcgtacg tcttgctgcg gtggtgctgc aaatcacacc acaagccaat cccgcaggcc
     9961 aattgctgct tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa agtgccttag
    10021 acaaaattct gagcgcgctg tgtcagcaag gactggcgat tgtgatgagc agtcacgatc
    10081 tcaaccacac attgcgtcat gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg
    10141 ccagtggacg cagagaagag gtgctcacgc cggcaaatct ggcgcaggcc tatgggatga
    10201 attttcgccg tctggatatc gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt
    10261 agtatcttga aaatcgggta attacgacgc taaattaatg caagaataaa aaacagagga
    10321 accgtacgga atgcgtttct gccttatttt gatcacagca ctgtttctgg ccgggtgtag
    10381 ccaccataaa gcaccgccgc caaatgccag actttctgat tcgattaccg ttattgccgg
    10441 tttgaacgac cagctacaaa gctggcatgg cacgccgtat cgttatggtg gcatgacgcg
    10501 gcgcggtgtg gactgttcgg gatttgtggt tgtgacgatg cgcgatcgtt tcgatttgca
    10561 gctgccccgt gaaaccaaac aacaagcctc tattggcacg caaattgata aagacgagtt
    10621 gctgccaggt gacctggtct ttttcaaaac gggttccgga caaaatggtt tgcatgtagg
    10681 tatttacgat accaacaacc aatttatcca cgcctctacc agcaagggag tgatgcgttc
    10741 ctcacttgat aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt
    10801 cga
//
LOCUS       DAZFRG010000165        10790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFRG010000165 DAZFRG010000000
VERSION     DAZFRG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.165"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..485
                     /gene="yiaM"
                     /locus_tag="ACHKUC_003432"
     CDS             18..485
                     /gene="yiaM"
                     /locus_tag="ACHKUC_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO6619634.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            488..1765
                     /gene="yiaN"
                     /locus_tag="ACHKUC_003433"
     CDS             488..1765
                     /gene="yiaN"
                     /locus_tag="ACHKUC_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO6619635.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1778..2764
                     /gene="yiaO"
                     /locus_tag="ACHKUC_003434"
     CDS             1778..2764
                     /gene="yiaO"
                     /locus_tag="ACHKUC_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO6619636.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGAIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVFLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2768..4264
                     /gene="lyxK"
                     /locus_tag="ACHKUC_003435"
     CDS             2768..4264
                     /gene="lyxK"
                     /locus_tag="ACHKUC_003435"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO6619637.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQKDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHKPERYAQIGCVMMTH
                     DYLRWCLTDVKGCEESNISESNLYNMGRGEYDPYLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4261..4923
                     /gene="ulaD"
                     /locus_tag="ACHKUC_003436"
     CDS             4261..4923
                     /gene="ulaD"
                     /locus_tag="ACHKUC_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6619638.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAGDFHAQIDSIWGGK
                     RA"
     gene            4916..5776
                     /gene="sgbU"
                     /locus_tag="ACHKUC_003437"
     CDS             4916..5776
                     /gene="sgbU"
                     /locus_tag="ACHKUC_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO6619639.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5770..6465
                     /gene="araD"
                     /locus_tag="ACHKUC_003438"
     CDS             5770..6465
                     /gene="araD"
                     /locus_tag="ACHKUC_003438"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6619640.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6512..8050)
                     /gene="aldB"
                     /locus_tag="ACHKUC_003439"
     CDS             complement(6512..8050)
                     /gene="aldB"
                     /locus_tag="ACHKUC_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO6619641.1"
                     /translation="MTNNPPSARVQPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTIEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8158..9453)
                     /locus_tag="ACHKUC_003440"
     CDS             complement(8158..9453)
                     /locus_tag="ACHKUC_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO6619642.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(9583..10734)
                     /gene="yiaY"
                     /locus_tag="ACHKUC_003441"
     CDS             complement(9583..10734)
                     /gene="yiaY"
                     /locus_tag="ACHKUC_003441"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO6619643.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNILTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         2494 a         2878 c         2827 g         2591 t
ORIGIN      
        1 aaccagaagg actcgttatg aaacggatac tcgaaggcat tcttgcgatc atcatcgcaa
       61 tactgtcttg tattattttt attaatatca ttcttcgata tggattccac acaagtatat
      121 tatcgattga tgaattatcc cgacttttat ttgtctggtt aacctttatt ggcgcaatcg
      181 ttgcttatat ggacaacagc catgtgcagg ttactttttt agtcgaaaaa ctctcaccgg
      241 ctaatcaaca acgtgtttcg ctgctaaccc atacgctcat tttattgctg tgtctggggc
      301 tggcctgggg cgctatagaa aaaacagcgc aagactggag caacttatcg cccattcttg
      361 gcgtccccgt tggcttaatg tacgccgccg ctatccccac cagtctgatt atcgcattgc
      421 tggaattgcg tcacttatac cggcagttca caaatacccc cagccgaaac caacaaggag
      481 cctgacgatg acaatcctta tatttattgt cagtttgtta ggggctatcg caattggcgt
      541 tcccatcgcc tgggcattat tactgtgcgg catttcattg atgttctgga tggatatctt
      601 tgatgtgcaa attctggcac aaacgttggt aaatggcgca gacagcttct ccctgttagc
      661 catccccttc tttgtactgg cgggtgagat catgaacgcg ggcggcctgt cgcaacgtat
      721 cgtcgacttg ccaatgaaac tggtaggcca caggccaggc ggtctgggtt acgtgggcgt
      781 gctggcagcg atgatcatgg caagcctgtc cggttctgcg gttgctgata ccgcagctgt
      841 cgcggcgctg ctggtgccga tgatgcgcca ggccaactat ccggtaaacc gcgccgccgg
      901 gcttatcggt tccggcggga ttattgcggc cattatcccg ccttccatac cactcattat
      961 cttcggcgtc tccagcggac tctccatcag caaactgttt atggcgggca ttgcgccagg
     1021 catcatgatg ggcgtcacgc tgatggttac ctggtggtgg caggcaaaac gactgaattt
     1081 accttgtcag ccgaaagcct cactgcgaga agtctggcaa tccttagttt ccggtatttg
     1141 ggcgctcttt ttaccgataa ttattattgg cggtttccgt tcggggttat ttacaccgac
     1201 cgaagcaggc gcggttgccg ccttctacgc tctgttcgtc tcggtggtgg tctatcgcga
     1261 gatgaccttc tccacgcttt atcatgtgct gattaacgcg gcaaaaacca catccgtggt
     1321 gatgttcctg gtggcctctg ctgcggtatc tgcatggctt attacgatcg ccgaattacc
     1381 catgatggta tcggagctat tacaaccgtt ggttgattcg cctcgcttat tattcatcgt
     1441 cattatgctc gctattatgg tgatcagtac ggtcatggac ttaacgccta ccgtattaat
     1501 tcttacacca gtattaatgc cattagtgaa agaagcggga atagatccgg tctatttcgg
     1561 catcatgttt attatcaact gttcaatatc actgattacg cctccagtcg gtaatgttct
     1621 taacgtggta tgcggcgtgg cgaaacttaa atttgacgat gcggtaaaag gcgttgcgcc
     1681 ttacgtcatg gttttattca tgttgttagc attatttatt tttataccgg agctcattac
     1741 cgctcctctt aaatggatga gttaaaagga aatcataatg aaattacgtt ctgtaaccta
     1801 cgctttatta attgccggag tggcgacgtt cagcacatct tctctggcgg cacaatcatt
     1861 acgtttcggt tatgaaacct cacaaaccga ttcacaacat attgcggcga aaaaatttaa
     1921 tgaattactg caggaaaaaa ccaaaggcga actgaaatta aaactgttcc cggatagcac
     1981 ccttggtaat gcgcaggcga tgatcagcgg cgtgcgtggc ggcgctattg atatggagat
     2041 gtctggctct aacaacttta cgggactctc gcccgtcatc aacctgctcg atgtcccgtt
     2101 cctgttccgc gataccgctc acgcgcataa aacgctcgac ggcaaagtcg gtgatgacct
     2161 gaaagtcttc cttgaaggta aggggctgaa agtactggcc tactgggaaa acggctggcg
     2221 cgatgtcacc aactcgcgtg caccggtcaa aacgcctgcc gacctgaaag ggttgaaaat
     2281 ccgcaccaac aacagcccga tgaatatcgc tgcatttaaa gtttttggcg ctaacccgat
     2341 cccgatgccg tttgccgaag tctataccgg gctggaaacc cgcactatcg acgctcagga
     2401 acacccgatc aacgtcgtct ggtcagcaaa attttatgaa gtgcagaagt acctttctct
     2461 gacccatcac gcctattcgc cgcttctggt ggtgatcaac aaagcgaagt ttgatggctt
     2521 aaccccggag ttccagcaag cactgatctc atctgcacag gaagcgggta gctatcagcg
     2581 caaactggtc gccgaagatc agcaaaaaat catcgacggt atgaaagaag cgggcgtgga
     2641 agtcatcacc gatctcgacc gcaaagcctt tagcgatgcg ctgggtaccc aggtccgtga
     2701 catgtttgtt aaagatgttc cgcagggtgc tgatctgctg aaagccgtgg atgaggtgca
     2761 ataaaccatg acgcaatact ggctggggtt agattgtggc ggtagctggc tgaaagccgg
     2821 gctgtatgac cgagaaggcc gggaggcggg cgtgcagcgc ctgccgctgt gcgcattaag
     2881 tccacagcca ggctgggcag agcgcgacat ggcagaactg tggcaatgtt gcactgctgt
     2941 cattcgcgcc ctgcttaccc attctggcgt cagcggggaa caaattgtcg gtatcggcat
     3001 ctccgcgcag ggaaagggct tatttctact tgataaaaac gacaaaccgc tcggtaacgc
     3061 cattttgtcc tcggatcgcc gggcgatgga aatcgttcgt cgctggcaga aagatggcat
     3121 cccggaaaaa ctctatccgc tgacccgaca aaccttatgg accgggcatc cggtgtcgct
     3181 gttacgctgg ctgaaagagc acaaaccaga acgctacgcg caaattggct gcgtgatgat
     3241 gacgcacgac tacctgcgct ggtgtttaac cgacgtcaaa ggttgcgaag agagcaatat
     3301 ttctgagtcc aatctctaca acatgggtcg cggggaatat gacccgtacc ttaccgactg
     3361 gctggggatc gctgaaatca atcacgccct gccgcctgtt gtcggatctg ccgaaatctg
     3421 cggggaaatc accgctcaga cagccgtact gaccggtctg aaagcgggta cccccgttgt
     3481 cggcggcctg tttgatgtgg tttccaccgc actctgcgcc gggctcgaag acgaattcac
     3541 cctcaatgcg gtgatgggga cctgggcggt gaccagcggc ataacccacg gtttacgtga
     3601 cggtgaagcg catccgtatg tctatggtcg ctacgttaac gatggtcaat ttatcgttca
     3661 cgaagccagc cccacctctt ccggcaatct cgaatggttt accgcacagt ggggagaaat
     3721 ctcatttgct gagattaatc aggccgttgc cagtttgccg aaagccggtg gcgatctctt
     3781 tttcctgccg ttcctgtacg gcagcaacgc cggactcgaa atgaccagcg gtttctacgg
     3841 gatgcaggcc attcacaccc gtgcacacct cttacaggcc atctatgaag gcgtggtgtt
     3901 cagccatatg actcatctca accggatgcg cgaacgtttt actgatgtgc acaccctgcg
     3961 cgtcactggc ggcccggcgc actccgatgt ctggatgcaa atgctggcgg acgtcagcgg
     4021 tctgcgtatc gagctgccgc aggtggaaga aaccggctgc tttggtgcgg cacttgccgc
     4081 ccgcgtcggt accggggttt atcgcgattt cagcgaagcc caacgtgact tacagcaccc
     4141 ggtacgcacc ctgctgccgg atatggccgc gcatcagctt taccaacaaa aataccaacg
     4201 ctatcaacat ctcattgccg cacttcaggg ttatcacgcc cgcatcaagg agcacacatt
     4261 atgagtcgac cacttctgca actggccctc gaccactcat cacttgaagc cgcgcagcgc
     4321 gacgtgacgc ggttaaaaga cagcgtcgat attgtcgaag caggcaccat tctctgttta
     4381 aacgaagggc ttggcgccgt gaaagccttg cgcgaacagt gccctgacaa aatcatcgtt
     4441 gctgactgga aggtcgccga cgcgggtgaa acgctcgcgc aacaggcatt tggcgcaggc
     4501 gctaactgga tgaccatcat ctgcgccgcg ccgctcgcca cggtagaaaa aggccacgcg
     4561 atggcacaga actgcggtgg tgaaattcag atcgagctat tcggtaactg gacgctggac
     4621 gatgcccgtg actggcatcg tattggcgtg cggcaggcca tttatcatcg tggtcgtgac
     4681 gcgcaggcca gcgggcaaca gtggggcgag gccgatctgg cacgcatgaa ggcgctttcc
     4741 gatatcggcc ttgagctttc cattaccggc gggattaccc ctgctgacct gccgctgttt
     4801 aaagagatcc gcgtgaaagc gtttattgcc ggacgggcgc tggcaggcgc agcaaatccg
     4861 gcgcaagtgg ctggcgattt ccatgcgcaa atcgactcca tctggggagg caagcgtgcg
     4921 taatcatccg ttaggcattt atgaaaaagc gctggcgaaa gatctctcct ggccggagcg
     4981 gctggtgctg gcaaaaagct gtggttttga ttttgtcgaa atgtcggtgg atgaaaccga
     5041 cgaacggttg tcacgtcttg actggagcgc cgcacaacgt acttcgctgg ttaccgcgat
     5101 gatcgaaacg ggtgttgcta ttccgtcaat gtgcctgtcc gcacatcgtc gttttccttt
     5161 tggtagccgg gacgacgccg tgcgccagcg ggcgcgggag attatgagta aagcgattcg
     5221 cctggcgcgc gatctcggca ttcgtactat tcaactggca ggttacgacg tctattacga
     5281 agaccacgac gaaggcaccc agcaacgttt tgctgaaggg ctggcgtggg cggtcgaaca
     5341 ggcggcggca tcacaagtca tgctggcagt ggagattatg gacaccgcgt ttatgaactc
     5401 catcagcaaa tggaaaaaat gggacgagat gctcgcctcg ccgtggttca ccgtctaccc
     5461 ggacgtcggc aacctcagcg cctggggcaa tgatgttccc gccgaactga aactgggcat
     5521 cgaccgtatc gccgccatcc acctgaaaga cactcaaccc gttaccgaac atagccccgg
     5581 acagttccgc gatgtgccgt ttggcgaagg ctgtgtcgat tttgttggca tcttcaaaac
     5641 gctgcatgag ctgaactatc gcggatcatt tctgattgaa atgtggaccg aaaaagccaa
     5701 agagccggtg ctggagatta ttcaggcgcg gcgctggatt gaagcgcgta tgcaggaggc
     5761 cggatttata tgttagagca actgaaagct gacgtgctgg cggcaaacct ggcgcttccc
     5821 gttcatcatc tggtgacgtt cacctggggc aatgtcagcg cgatagacga aacgcggcaa
     5881 tggatggtga ttaagccttc cggcgttgag tatgaggtaa tgaccgccga cgatatggtg
     5941 gtggttgaga tagccagcgg caaggtggtg gaaggcagca aaaagccctc ttccgacacg
     6001 ccaacgcatc tggcgcttta ccgtcgcttt gcggagattg gcggcatagt gcatacccac
     6061 tcgcgccacg ccaccatctg gtcgcaggcc ggacttgatc tccccgcctg gggcactact
     6121 cacgccgatt atttttacgg agccatcccc tgcacgcgcc tgatgaccgc agaagagatc
     6181 aacggcgagt atgaatacca gaccggcgaa gtgatcatta aaaccttcga agagcgtggg
     6241 ttgaacccgg cacaaatccc ggcggtactg gtgcattctc acggcccgtt cgcctgggga
     6301 aaaaatgccg ccgatgcggt gcataacgcc gtggtactgg aagagtgcgc ctatatgggt
     6361 ctgttctcgc gccagcttgc gccacagctc cctgcgatgc aaaacgaact gctggataag
     6421 cactacctgc gtaagcatgg ggccaatgcc tattacgggc agtagccatg cctgagagtg
     6481 cccggcaata cacgccgggc agcctttcac atcagaacag ccccaacggt ttatccgagt
     6541 agctcaccag caggcacttg gtttgctggt aatgctcaag catcattttg tgggtttcgc
     6601 ggccaatgcc tgattgtttg tagccgccaa acgccgcatg tgccgggtaa gcgtgataac
     6661 agttggtcca cacgcgccca gcctgaatgc cgcgccccat cttataggcc agattaccgt
     6721 tgcggctcca gacgcccgcg cccaggccat attgcgtatc gttcgccagc tccagcgctt
     6781 cttctatcgt tttgaaggtg gttaccgcca gcaccgggcc aaaaatctcc tcctggaaca
     6841 cgcgcatatt gttctgacca aacagaatcg tcggttcgag gtagtagccg tctttcagtt
     6901 cgccttccag cagcttgcgt cgcccgccgg tgagcacatc agcgccctct tttttaccga
     6961 tatcaatgta gttgaggatg gtttccagtt gcccgtgaga aacctgcgcg cccatttgcg
     7021 tcacgctgtc gagcgggtta ccgctgcgaa tgctttcgac acggcggata gcgcgttcca
     7081 taaagcgttc gtagatagac tcctgcacta aagcacggct tggacaggtg caaacttcgc
     7141 cctggttaaa ggcaaacagt gcaaagcctt ccagcgcttt atcaaaaaag gcatcttctt
     7201 catccatcac atcagcaaag aagatatttg gcgatttgcc gcccagctcc agcgtcaccg
     7261 gaataatgtt ttgcgtggcg tattgcataa tttgttggcc cacttccgtt gagccagtaa
     7321 acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc ccacctgcgc
     7381 cgttgaccac gttcaccacc cctggcggca gtaaatcacc gacgatttcc attagcagca
     7441 gtacagaaag cggagtaaga cgtgcgggtt tcagcaccac acagttgccc gccgccagcg
     7501 cgggagccat tttccagctc gccatcagca gcgggaagtt ccacgggata atctgcccca
     7561 ccacgcctaa cggttcatgg aaatgatagg ccacggtttc gctatccact tcactgatcc
     7621 caccttcctg cgcccgaata cacgaggcga aatagcgaaa atgatcaatc gccagcggca
     7681 cgtcggcagc actggtttcg cgaatgggtt tgccgttatc ccaggtttct gccgtcgcta
     7741 aaagttcaag gttttgttcc attcgatcgg caatcttaaa cagaatcgcc gcgcgatcct
     7801 gcaccgaggt gtgcgcccat ttatctttca ctttatgcgc agcatccagc gccagatcga
     7861 tgtctcgttt gcccgaagac gccacttcgc acagtagctg cccggtcacc ggcgtcaggt
     7921 tctggtaata ctcgccatcg gcaggcgcta cccattcgcc accgataaag ttgtcgtagc
     7981 gggcttttaa tttgaggggg aaaccatact cgccgggctg aacacgtgct gaagggggat
     8041 tattggtcat gatcgtctcc ttgcggtgtg aggtataaca agggtagacg taactggcga
     8101 aatcttcgcc agtcggtgac agctttacga cggctatcac gaatttacgg gcaagggtta
     8161 cagcggataa tgcgcgaagt acacggcttg ctggtcatta attttcttct cttgcgcaaa
     8221 cccttcttta acaattgtcc gtaaatcagc tcgagccgta ttatcagaga cgcccaagct
     8281 cgcgctaatt tcagcaactg tgtactcttt ccccggattg gcgagcatca cattcaggag
     8341 cgttacctgg cgcggattca ggcgtttaag cacaccagag ctaaacagca cactatcgag
     8401 ttttgcgctt cgttgtgtaa tttcatcgat atgctgctgc cagttttgca gagcacgttt
     8461 aatgacaccc gcctgatatt caaggaaata agtcagatcc ataccatctg attcagtgta
     8521 ctggtagctg gcggcatatt tgaccggtgc agcatgaagc aaacggctga tggaaatata
     8581 tttaaagaca tcgtagcccg atttcagcat ataccagtaa aacagcgccc gactggtgcg
     8641 cccgttgcca tcccgaaacg gatgttcatg ggccagcata aaatgcagaa tgcaggcacg
     8701 gaccaacgga tgaatatacc cttcatggct gtttaaccac tggcaaactt tttccagccg
     8761 ttccggtaac aatgccgcag cgggcggttg atgcactata ttgccatcat agtcggcgat
     8821 cacaacatca tctgtttcac gaaattctcc cggacgatat ttcgcgtcat tgattccacc
     8881 cataccaatg gcatgtaact ggcgaataag cgcaggtgtc agcggttctg cgagtaagtg
     8941 cggaatttcc gccatcagcc gggcatttcc cgcaatcata tgctcgtctt cagtgcgtgg
     9001 cgaccgccct gtttccagca tttttctcgc caccagcgtt gtagtattcg caccttccag
     9061 ctgggagctg gtgatcggct cttccaggcg taactggctt aactcggctc cggccccgcg
     9121 taaccgttca gtctgatctt tcagcgccag cgcggtagca cgtttgtcga caagctcgca
     9181 cgcttctgca atcaccgggg taatgttgaa tccggcctgt tcacccgctt cattgcaata
     9241 attaattcgt tggatcgccg cgttgcgcgc cagacgggta agcgtccacg ccacatcgac
     9301 gttttctcct tttttgatgc ggtactgcaa ctcatcaaaa ggcagataac gtcctttttc
     9361 atcggtaacc cggttaaagc gcaggtgggc caggagttcc tcacccgata agtgtgataa
     9421 aggaataagt ccgggaggtg atttgatagc catgtgctta tctcaaaaaa gcgcaatatt
     9481 gagatagtgt atagcaggaa agagaaacta tctcaaaaaa agcaagtttt gagatagttg
     9541 aaaaagcggc tgacaactcg ccagccgttg tggaaatgat gattacatcg ctgcgcgata
     9601 aatcgccaca atttcttcgt gagttgcctg gatcgggtta gtaaagccgc aggcatcttt
     9661 cagggcatta gtcgccagaa ccgcgaaatc ttcttctttc acgttcaggt cgcgtaggcc
     9721 tgccgggata tccactttct tcgccagttc acggatggcg ttaatgcagg cttcagcacc
     9781 ttccgcgtcg tttttacctg tcacgttcac gcccattgca gcggcacaat cacgcagacg
     9841 tgcagcggcg actttgctgt tgaatacctg aacatgcggc agcaaaacgg cgttacatac
     9901 accgtgtggc aggttgtaga aaccgcccag ctggtgcgcc atcgcatgaa cataacccag
     9961 agaagcatta ttgaacgcca taccggcgag gaactgggca taagccattg cttcacgcgc
    10021 tttcgcatta ctgccatctt caacggctaa cggcaggttt tcggcaatca tggtcacggc
    10081 tttcagtgca caagcgtcag tgatcggcgt ggcggcaata gaaacatacg cttcgatagc
    10141 gtgcgttaag gcatccatac ccgttgcggc ggtcagtgac ttcggcatac caatcatcag
    10201 agaggagtca ttgacagaaa gcaacggagt gacatgttta tccacaatcg ccattttgat
    10261 gtgacgcgct tcgtcagtga tgatgcagaa acgggtcatt tcagaggccg tacccgccgt
    10321 ggtgttgatg gcgatcatcg gcagctgcgg ttttgcagag cggtcaacgc cttcataatc
    10381 gcggatgtca ccgccgttgg ctgcaaccag cgcaatacct tttgcgcagt cgtgtggaga
    10441 accaccgcct aaagaaatca cgctatcgca attattttct ttaagtaatt tcaaacctgc
    10501 agcgacgttt tccgtggtgg ggttaggttg ggtgccatca taaataacgc taaaaatatt
    10561 gcgttcttcc agtgcttttt gcacatcacc cgccataccc aatttcgtta atatgttgtc
    10621 ggtgacaatt aaggtacggg taaatccata atctgccatc atattcattg catcattcaa
    10681 tgaatcagca ccaataacat tcacggaagg aataaaaaag gttgaagctg ccataacaca
    10741 ctcctgaaag tgtaaaaaag ggcgggcagc ttactgagga ttttcatttg
//
LOCUS       DAZFRG010000166        10773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFRG010000166 DAZFRG010000000
VERSION     DAZFRG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.166"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..608)
                     /gene="mog"
                     /locus_tag="ACHKUC_003442"
     CDS             complement(21..608)
                     /gene="mog"
                     /locus_tag="ACHKUC_003442"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO6619644.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(723..1676)
                     /gene="tal"
                     /locus_tag="ACHKUC_003443"
     CDS             complement(723..1676)
                     /gene="tal"
                     /locus_tag="ACHKUC_003443"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6619645.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGDIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            1955..3385
                     /gene="yaaJ"
                     /locus_tag="ACHKUC_003444"
     CDS             1955..3385
                     /gene="yaaJ"
                     /locus_tag="ACHKUC_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO6619646.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     CLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            3455..4231
                     /gene="yaaA"
                     /locus_tag="ACHKUC_003445"
     CDS             3455..4231
                     /gene="yaaA"
                     /locus_tag="ACHKUC_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO6619647.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNAQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(4384..4680)
                     /gene="yaaX"
                     /locus_tag="ACHKUC_003446"
     CDS             complement(4384..4680)
                     /gene="yaaX"
                     /locus_tag="ACHKUC_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO6619648.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4894..6180)
                     /gene="thrC"
                     /locus_tag="ACHKUC_003447"
     CDS             complement(4894..6180)
                     /gene="thrC"
                     /locus_tag="ACHKUC_003447"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO6619649.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEISQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(6181..7113)
                     /gene="thrB"
                     /locus_tag="ACHKUC_003448"
     CDS             complement(6181..7113)
                     /gene="thrB"
                     /locus_tag="ACHKUC_003448"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO6619650.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(7115..9577)
                     /gene="thrA"
                     /locus_tag="ACHKUC_003449"
     CDS             complement(7115..9577)
                     /gene="thrA"
                     /locus_tag="ACHKUC_003449"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO6619651.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLMRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9603..9722)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9658..9723)
                     /gene="thrL"
                     /locus_tag="ACHKUC_003450"
     CDS             complement(9658..9723)
                     /gene="thrL"
                     /locus_tag="ACHKUC_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO6619652.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9937..10623)
                     /gene="yjtD"
                     /locus_tag="ACHKUC_003451"
     CDS             complement(9937..10623)
                     /gene="yjtD"
                     /locus_tag="ACHKUC_003451"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6619653.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAA
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         2526 a         2897 c         2650 g         2700 t
ORIGIN      
        1 ccccgcttgg ggggattttt ttattcgcta acttcgcgtc ttgcactctt cggtctgaat
       61 gctgcaacca cttccggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac
      121 ggtacgctgg caaaaatacc gtgcaccaca acgttaccct cagcgtcctt cacaccttcc
      181 agcgtctctt taatagactt cggttgaccg ggtaagttaa ggatcagcgc ctgtttgcga
      241 atcaccccca cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc
      301 atctgttcac caaagcctgg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt
      361 acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca
      421 tccaccagct cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt
      481 tccagttcaa acggcgtggt tagcgccgat gtcagccatt cttccagcgc agggatgcct
      541 ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa
      601 gtattcatca agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag
      661 ggagtgacag acaggtttca gaagaggtag cgtgaccgac ttcccggtca cgctaagtat
      721 gattacagca gatcgccgat cattttttcc agtttttcct ggtcaacagc aaacttacgg
      781 ataccttccg ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg
      841 gactcagtga tacgcgccgg acgcgctttc acttcaccag tgtaagacag tttacgttcg
      901 atatcccctt cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtcg
      961 cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt
     1021 tcataaccat gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct
     1081 tctgccggag cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca
     1141 acaaacggcg agatcaggaa cacgcccgct tccgcacaag cacgagcctg agcgaaggag
     1201 aacagcaggg tcaggttaca gttgatgcct tctttttcca gctgttccgc agcacggata
     1261 ccctgccagg tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg
     1321 tagagtttga tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc
     1381 gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag accaatattt
     1441 acggccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc
     1501 gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga
     1561 atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc
     1621 ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata
     1681 gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg
     1741 acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt
     1801 tctgacactg cgttgatatc gcccgccatt tttatacaaa acctcatgta tgctacgcag
     1861 aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg
     1921 aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa
     1981 cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac
     2041 ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa
     2101 tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc
     2161 agcgcgcgtg ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc
     2221 tggagccgtc ttctggatgt gggttgccgc atttatcggc atggcgacct cgtttgccga
     2281 atgttccctc gcacagcttt ataaagaacg tgatgccaat gggcagtttc gtggcggacc
     2341 ggcatggtat atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt
     2401 tttgctcatc gcctacgcca taattttcag cggagttcag gcgaacgccg ttgcgcgagc
     2461 cctgagtttt tcttttgatt ttcccccgct ggtgacaggt attattctcg ctgtctttgc
     2521 tatgctggcg atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc
     2581 gttgatggcg ataatctggg tactgacctg cctggtgatt tgcgtaatga atatcgggca
     2641 acttccccac gtcatttggt ctatttttga gagtgctttt ggctggcagg aagctgcagg
     2701 cggcgcggcg ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt
     2761 ttccaatgag gcgggaatgg ggtcgacgcc aaacgcggca gcggcagctg cgtcctggcc
     2821 tccgcatccg gcagcgcaag gaattgtcca gatgattggc atttttatcg acaccctggt
     2881 catctgcacg gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc
     2941 gctggaaggt attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc
     3001 ggagtttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat
     3061 ttatgccgaa aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgtct
     3121 gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagtcttc cgctgatgtg
     3181 gcaactggca gatatcatta tggcctgcat ggctattacc aatttgaccg ccatattact
     3241 gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt
     3301 gcgcccggtg tttgatccgt tgcgttatcc ggagatcggt cgccagcttt ctccagacgc
     3361 gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc
     3421 taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa
     3481 aacgcttgat taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga
     3541 caacgcccag cagctgatcc atgaagcgcg gaaactgacg cccccgcaga ttagctccct
     3601 gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc
     3661 agatttcaca ccggagaatg cccgccaggc aattctggcg tttaaaggtg atgtctacac
     3721 tggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg
     3781 aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct
     3841 ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga
     3901 tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg tagtgataaa
     3961 tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat
     4021 caagccagtg ttcctcgatg agaagaacgg caaatttaag ataatcagct tctacgctaa
     4081 gaaagcacgc ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca
     4141 actgactggt tttaatagcg aaggttactt ctttgatgaa gcttcctcca gcaatggcga
     4201 actggtgttt aaacgctacg agcagcgtta agtttgtagt caatgtcgga tgcggcgtga
     4261 acaccttatc cggcctacaa aagcatgcag attcaatata ttgcagaatt cacgtaggcc
     4321 tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg gatagttacc cggcatttgt
     4381 tatttagcgg tgatgtttgc ctggaccatg accgccgtga tgatcatgag gagctttctt
     4441 atggtggcgc ggcgatggcg gcggtccata tgggtgccag cgattgcctc gccattcata
     4501 atgttgtttc caccagccgt ggtcgcgcca gtgaccgcca tcccagtaat agccacgatt
     4561 atcacgatcg cctatctgta attttactga cgggactagc gtaatttccg cagcctgcgc
     4621 tgccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac
     4681 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
     4741 cggcagttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gagaatttct
     4801 ccttttttcc tgtcgttttt ctccatattt tcttcataaa aaagccgggc tgcataaaag
     4861 caaacccggc ctgattgaga taatgaatag atgttactga tgattcatca tcaatttacg
     4921 caacgcagca aaatcggcgg gcaggttatg cgaaagcaag ggtaaatcag cacgttctgc
     4981 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
     5041 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag
     5101 cgcacgataa gctacggcag cgtgcggctc cgaggtatag cccagttctt ttaactcacg
     5161 cattgtctgt tgcgtggttt catcatccac ggctgcatac cccagctctt tcagttgcca
     5221 gattttgcgg cggaacaact cttccacacg cggccagttg tttggctggc taacatccat
     5281 cgcattggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
     5341 tggtacggta tcgttcacgt tggtcgcagc aataaaacgt tttaccggca gaccgagtga
     5401 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agactaccag
     5461 ctggttgcgc gcttcctgcg gcagctgcgc aacagcttca aagtagtagc aaatctgcgc
     5521 cagcaaacgg ctgatgttaa tcgagttagc tgagtttaac cccagcgcca ctttcagttc
     5581 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
     5641 aacagtttcg atattgccgc ccaatgtaca gaacagtttt tcttgcagtg gactgatttt
     5701 gcctcgtgga tagaggataa ccactttcac attcggtaaa ccgtagaaag catgagccac
     5761 tgccgctcca gtatcaccgg atgtcgcggt cagaatggtc actggcttat cgcccgcaat
     5821 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
     5881 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc
     5941 aaacgccgca cgtacgcgct cttccaggat ttcctgcgag atttcatcac caataaacgc
     6001 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
     6061 caggctgaat tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg
     6121 ggttacggct tgcgcaaagc tgacctgctc attgtgatct ttcagattgt agagtttcat
     6181 ttagttttcc agtactcgtg cgcccgccgt atccagctgg caaatatgaa caaaaccttc
     6241 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
     6301 gtcacacaga gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat
     6361 ttccgcaacc gcctgccgcg cctgccggaa gccaggcaat aaccgttcac ggtagggttc
     6421 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
     6481 aatgaagcct gccaaatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
     6541 taaaatagcc ctggcttctg ccgtcgagac tttaatcccc ggatacgcca gcacccacag
     6601 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
     6661 cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgccc
     6721 ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc cacagtgttc
     6781 attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat
     6841 cggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct cctggcaaaa
     6901 acgctcccag cactgataaa cgatattttc ccgtggctct gacggcagct tatcggcaaa
     6961 gcgtccgagg ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
     7021 caatgcacca tcaacaggtg tcaccgccgc cccgagcaca tcaaacccga cgctcatatt
     7081 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
     7141 tacgtagcag atcagcaaag acaccggcag ctgtaacgtc attgcccgca ccatatccgc
     7201 gcagtaccaa cggaagcggc tgataatagt ggctatagaa ggccagggcg ttttcgccat
     7261 ttttcacttt gaacagcgga tcattaccat ccacttcggc aatcttcacg cggcagacgc
     7321 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
     7381 cacgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcgacatcac
     7441 cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt
     7501 cacgtcccgt ttcacgagcg agaatcaata gcttacgcgc cacatccata ccagaaagat
     7561 catctcgcgg gtccggttcg gtataaccca tttcccgcgc cagtgtggtc gcctcggaga
     7621 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg
     7681 agaacttcat caattcatca ccagcattga gcagattttg caggttctca ataaccggta
     7741 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcataac
     7801 gcaactgatg gtagtaatcc atcgacgagg tgttggcctt tttgttcggc gtaacaacgt
     7861 ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaagtacagt
     7921 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgcattaag cgcccgagat
     7981 taaacggctc tttggcttgc gccagttctt cctgccagtt ttccagatta aggccatgta
     8041 cattggtgag cagtgccttc gagttagcaa caccgcagac acgtaagtcg atatgtttat
     8101 tcttcaacca gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
     8161 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
     8221 gcacgccagt ggtcgcatca tcgttattga ccacgacaga gattgagcgt tcagaagatc
     8281 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
     8341 agatcccacg taaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
     8401 tcaccgccaa cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
     8461 cccgttcagc tcgcacacag tcgctttgcg gaacgcagaa actgatactg tattcggaag
     8521 atgattgcgt aatcagcacc acggaaatac gggcgcgtga catcgctgca aagacgcgcg
     8581 ccgccatgcc aaccatccct ttcatccccg ggccggaaac gctgaacatt gccatgttat
     8641 tcagattgga aatgcccttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
     8701 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctga aactgggcga
     8761 tgggggtaat tgtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
     8821 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
     8881 aaacaccgtc aacatccgtc cagatctcgc aacaatcggc gcgtaaacag gccgccagca
     8941 ccgcagcgga gtagtcggaa ccgttgcgtc caagcaccac cagctcgcct ttttcattac
     9001 cggcagtgaa accagccatc agcaccatgt ggtcagccgg aatgcggctt gccgcaatac
     9061 ggcgggtgga ttcagcaata tcaacggtag attcgaggta atgccccact gcaagcagtt
     9121 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
     9181 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
     9241 gccccaacaa actgatgcca tgcaggacat gttttatttg ggcaaattcc tggtcgacga
     9301 aagttttcaa ttgtgccagc ggaaatcccg gctgggcggc ggcgagtccc gtcagaagtt
     9361 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
     9421 tttcaatcat cgctaccaga tggttggtaa ttttggcggg ggcagagagg acggtcgcca
     9481 cctgcccctg cctggcattg ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat
     9541 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
     9601 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
     9661 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
     9721 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
     9781 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
     9841 aagctgctat cagacactct ttttttaatc cacacagaga catattgccc gttgcagtca
     9901 gaatgaaaag ctgaaaatca cttactaagg cgttttttat ttggtgatat ttttttcaat
     9961 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
    10021 ttgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgtcat
    10081 ggctcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg
    10141 ttgttgtatt aatgttgcta attgatagca atagaccatc actgcctgcc ccagattgag
    10201 cgaaggataa tccgccacca tcggcacacc agtaagaacg tcagccaacg ctaactcttc
    10261 gttagtcagc ccggaatctt cgcgaccaaa caccagagcg gcatggctca tccatgaaga
    10321 tttttcctct aacagcggca ccagttctac tggcgtagcg tagtaatgat atttcgcccg
    10381 actgcgcgca gtggtggcaa cagtgaaatc gacatcgtgt aacgattcag ctagtgtcgg
    10441 gaaaacttta atattatcaa taatatcacc agatccatgt gcgacccaac gggcggctgg
    10501 ctccaggtgt gcctgactat caacaatccg cagttcgcta aaacccatcg ttttcattgc
    10561 ccgcgccgct gccccaatat tttctgctct ggcgggtgca accagaataa tcgttatacg
    10621 catattgcca ctcttcttga ccaaataacc gcgaacaggg tgatcattgt caacttatta
    10681 cgcgttgaga atttacaaat tcttaacgta agtcgcagga aaagcccttt gcttagctta
    10741 aaaaaagcta aactatttcc tgactgtact aac
//
LOCUS       DAZFRG010000167        10759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFRG010000167 DAZFRG010000000
VERSION     DAZFRG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.167"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..82
                     /gene="ychE"
                     /locus_tag="ACHKUC_003452"
     CDS             29..82
                     /gene="ychE"
                     /locus_tag="ACHKUC_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO6619654.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            113..760
                     /gene="ychE"
                     /locus_tag="ACHKUC_003453"
     CDS             113..760
                     /gene="ychE"
                     /locus_tag="ACHKUC_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO6619655.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(918..1079)
                     /locus_tag="ACHKUC_003454"
     CDS             complement(918..1079)
                     /locus_tag="ACHKUC_003454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619656.1"
                     /translation="MSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            1498..3129
                     /gene="oppA"
                     /locus_tag="ACHKUC_003455"
     CDS             1498..3129
                     /gene="oppA"
                     /locus_tag="ACHKUC_003455"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO6619657.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3215..4135
                     /gene="oppB"
                     /locus_tag="ACHKUC_003456"
     CDS             3215..4135
                     /gene="oppB"
                     /locus_tag="ACHKUC_003456"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO6619658.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            4150..5058
                     /gene="oppC"
                     /locus_tag="ACHKUC_003457"
     CDS             4150..5058
                     /gene="oppC"
                     /locus_tag="ACHKUC_003457"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO6619659.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GLLVVTLFCFNFIGDGLRDALDPKDR"
     gene            5070..6083
                     /gene="oppD"
                     /locus_tag="ACHKUC_003458"
     CDS             5070..6083
                     /gene="oppD"
                     /locus_tag="ACHKUC_003458"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO6619660.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            6080..7084
                     /gene="oppF"
                     /locus_tag="ACHKUC_003459"
     CDS             6080..7084
                     /gene="oppF"
                     /locus_tag="ACHKUC_003459"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO6619661.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(7137..7466)
                     /gene="yciU"
                     /locus_tag="ACHKUC_003460"
     CDS             complement(7137..7466)
                     /gene="yciU"
                     /locus_tag="ACHKUC_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO6619662.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7501..8961)
                     /gene="cls"
                     /locus_tag="ACHKUC_003461"
     CDS             complement(7501..8961)
                     /gene="cls"
                     /locus_tag="ACHKUC_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO6619663.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9104..9277
                     /gene="yciY"
                     /locus_tag="ACHKUC_003462"
     CDS             9104..9277
                     /gene="yciY"
                     /locus_tag="ACHKUC_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO6619664.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(9332..10585)
                     /gene="kch"
                     /locus_tag="ACHKUC_003463"
     CDS             complement(9332..10585)
                     /gene="kch"
                     /locus_tag="ACHKUC_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO6619665.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2792 a         2474 c         2662 g         2831 t
ORIGIN      
        1 gcaattttaa gtagttagga ggtgaaaaat gctgtcaaaa ggcgtattgt cagcgcgtct
       61 tttcaacctt atttatggct aacattatcc ggcttttgct tcggagctaa ccgtgattca
      121 gacccttttt gattttcccg tttacttcaa atttttcatc gggttatttg cgctggtcaa
      181 cccggtaggg attattcccg tctttatcag catgaccagt tatcagacag cggcagcgcg
      241 aaacaaaact aaccttacag ccaacctgtc tgtggccatt atcttgtgga tctcgctttt
      301 tctcggcgac acgattctac aactttttgg tatatcaatt gattcattcc gtatcgccgg
      361 tggcatcctg gtggtgacaa tagcgatgtc gatgatcagc ggcaagcttg gtgaggataa
      421 acagaacaag caagaaaaat cagaaaccgc ggtacgtgaa agcattggtg tggtgccatt
      481 ggcgttgccg ttgatggcgg ggccaggggc gatcagttct accatcgtct ggggtacgcg
      541 ttatcacagc attagttatc tgtttggttt ctttgtggct attgcattgt tcgctttatg
      601 ttgctgggga ttgttccgca tggcaccgtg gctggtacga gttttacgcc agaccggcat
      661 caacgtgatt acgcgtatta tggggctatt gctgatggca ttggggattg aatttatcgt
      721 tactggtatt aaggggattt tccccggcct gcttaattaa ttcctttcaa atgaaacgga
      781 gctgccatgc tccgtttact tcgtcattat ttttactttg ttcctgcgca gttatcaaaa
      841 gcaaaaggaa taggtaaaaa tattcttctc aaattacagt tagttataag gatttcctta
      901 actgcttctc ctcaccatca tgttattttc gccacatcat aatcctgggc ttgctgaaga
      961 ataattgaaa tgatattagt aattccactg cctttggtag aggaaagtgc taaataataa
     1021 tcaattgtta aattattgtg catttcactg cttgaactgt aatcagaaaa gagagacatg
     1081 cttaaccaac ctctatttga ttgaattgaa agatgtttgt taaggcatgg atgcaagcta
     1141 tagattctgg tacggtcaat aaaagagaat tgcttaacaa ttttgcaaaa tgtattggcg
     1201 agtaagaacc gcatttggta ctttccgggc aaccgccagg cgattcttta ttggtaatga
     1261 gaataattaa caattaaaga tcgtcgcgaa agaataatgt gtctcgacag gggagacaca
     1321 gtacgaatcg acataaggtg atcgtctgaa tcaccagaat aaataaagtc ggtgatagta
     1381 atacgtaacg ataaagtaac ctgacagcag aaagtctccg agcctgtgca gggtcccaat
     1441 ccgggattac acatgctggt taataccagt aattataatg agggagtcca aaaaacaatg
     1501 accaacatca ccaagagaag tttagtagca gctggcgttt tggctgcgct tatggcaggg
     1561 aatgtcgcgc tggcagctga tgtacccgca ggtgtcacac tggcggaaaa acaaacgctg
     1621 gtacgtaaca atggttcaga agttcagtca ttagatccgc acaaaattga aggtgttccg
     1681 gagtctaata tcagccgaga cctgtttgaa ggcttactgg tcagcgatct tgacggtcat
     1741 ccggcacctg gcgtcgctga atcctgggat aataaagacg cgaaagtctg gaccttccat
     1801 ttgcgtaaag atgcgaaatg gtctgatggc acgccagtta cagcacaaga ctttgtgtat
     1861 agctggcaac gttctgttga tccaaacact gcttctccgt atgccagtta tctgcaatat
     1921 gggcatatcg ccggtattga tgaaattctt gaagggaaaa aaccgattac cgatctgggc
     1981 gtgaaagcta ttgatgatca cacattagaa gtcaccttaa gtgaacctgt tccgtacttc
     2041 tataaattac ttgttcaccc atcaacttca ccggtgccaa aagccgctat cgagaaattc
     2101 ggcgaaaaat ggacccagcc tggtaatatc gtcaccaacg gtgcctatac cttaaaagat
     2161 tgggtcgtaa acgaacgaat cgttcttgaa cgcagcccga cctactggaa caacgcgaaa
     2221 accgttatta accaggtaac ctatttgcct attgcttctg aagttaccga tgtcaaccgc
     2281 taccgtagtg gtgaaatcga catgacttat aacaacatgc cgatcgaatt gttccagaag
     2341 ctgaaaaaag agatcccgga cgaagttcac gttgatccat acctgtgcac ttactattac
     2401 gaaattaaca accagaaacc gccatttaat gacgtgcgtg tgcgtaccgc actgaaattg
     2461 ggtatggacc gcgatatcat tgttaataaa gtgaaagcgc agggcgacat gcccgcctat
     2521 ggttacactc caccatatac tgatggcgca aaattgactc agccggaatg gtttggctgg
     2581 agccaggaaa aacgtaacga agaagcgaaa aaactgctgg ctgaagcggg ttataccgca
     2641 gacaaaccgt tgaccatcaa cctgttgtat aacacctccg atctgcataa aaagctggcg
     2701 attgctgcct cttcattgtg gaagaaaaac attggtgtaa acgtcaaact ggttaaccag
     2761 gagtggaaaa cgttcctcga cacccgtcac cagggcactt ttgatgtggc ccgtgcaggc
     2821 tggtgtgcag actacaacga accaacttct ttcctgaaca ccatgctgtc gaatagctca
     2881 atgaataccg cgcattataa gagcccggcc tttgacagca ttatggcgga aacgctgaaa
     2941 gtgactgacg aggcgcagcg tacagctctg tacactaaag cagaacaaca gctggataag
     3001 gattcggcca ttgttcctgt ttattactac gtgaatgcgc gtttggtgaa accgtgggtt
     3061 ggtggctata ccggcaaaga tccgctggat aatacctaca cccggaatat gtacattgtg
     3121 aagcactaat ggcaatacgt ggggcaggag tgtcctgctc cacggtgtct gatttttatc
     3181 gcattacaga aggcacaggc cagaaggtag ggcaatgtta aaatttattc tacgtcgctg
     3241 tctggaagcg attccgacgc tatttattct tattactatt tcgttcttta tgatgcgcct
     3301 cgctccgggc agtcctttta ccggcgaacg tactttacca ccggaagtga tggccaatat
     3361 cgaagcgaaa tatcatctta atgatccgat catgactcag tatttcagct acctgaaaca
     3421 actggcgcat ggcgatttcg gtccatcgtt taaatataaa gattattcgg tcaatgacct
     3481 ggttgcatcc agttttcccg tttctgccaa actgggagcc gcagcatttt ttcttgcggt
     3541 aatactgggt gttagtgctg gcgttattgc cgcattaaag caaaacacca aatgggacta
     3601 taccgtgatg gggctggcaa tgaccggggt tgttatcccc agttttgtgg ttgcgccatt
     3661 attagtcatg atatttgcga tcattttgca ttggctgccg ggcggtggct ggaatggtgg
     3721 agcgcttaaa ttcatgatat tgccaatggt ggcgttatca ctcgcctata tcgccagtat
     3781 tgcgcgtatt acccgtggtt ctatgattga ggttttacac tccaacttta ttcgtactgc
     3841 ccgggcgaaa gggttaccta tgcggcggat cattttacgc cacgcattaa aacctgctct
     3901 gttacccgtg ctctcctata tggggcccgc atttgtcggc attattaccg gttcgatggt
     3961 catcgaaacc atttatggtt taccggggat tgggcaattg ttcgttaatg gcgcattgaa
     4021 ccgtgactat tccttagtgt taagtctgac cattctggtt ggcgcgttaa ccattttgtt
     4081 taatgccatt gtcgatgtgc tatatgcggt tatcgacccg aaaatccgtt actgatactg
     4141 gagctcgcga tgatgttaag taagaaaaac agcgagacgc tggaaaattt cagtgaaaag
     4201 ctggaggtcg aagggcgcag cttgtggcag gacgcacgtc gacgttttat gcataaccgt
     4261 gcggcggttg ccagtctgat agtgctggtg ctgatcgcgt tatttgtaat cctggcaccg
     4321 atgctttcgc agtttgccta tgacgatact gactgggcga tgatgtccag cgccccggat
     4381 atggagtccg gtcactactt tggtactgac tcatccggtc gcgacctgct tgtgcgcgtt
     4441 gcgattggcg ggcgtatctc actcatggtc ggtgttgctg cggcactggt ggcagtggtc
     4501 gtggggacac tttatggttc gctttccggt tatctgggcg gtaaagtgga ttcggtaatg
     4561 atgcgtctgc tggaaatcct caactccttc ccattcatgt tcttcgtcat tttgctggtg
     4621 acctttttcg gtcaaaacat cctgctgatt ttcgtggcga ttggcatggt ttcctggctg
     4681 gatatggctc gtattgtgcg tgggcaaacc ctgagtctga agcgcaaaga gtttattgag
     4741 gcggcacaag ttggcggtgt atcgacgccg ggcattgtta ttcgccacat tgtgccgaac
     4801 gtactcggtg tggtggtggt ctatgcgtcg cttctggtgc ccagcatgat cctctttgaa
     4861 tctttcctta gcttcctggg attaggtacg caagagccgt taagcagctg gggtgcattg
     4921 ctgagtgatg gcgcgaactc gatggaagtt tctccatggt tactgttgtt cccagcggga
     4981 ctcctcgtgg tgacgctgtt ttgtttcaac tttatcggcg atggcttgcg tgatgccctc
     5041 gacccgaaag atcgttaagg agtgcagcca tgagcgtaat tgaaactgca actgtgccgc
     5101 tcgcacaaca acaggctgac gcactgctga acgtgaaaga tttgcgtgtc acctttagta
     5161 ccccggatgg cgacgtcacg gcggtcaatg atttgaattt ttcgctacgt gccggagaaa
     5221 cgctgggcat cgtaggtgag tctggttcgg gcaaatcgca aaccgcattt gcgttgatgg
     5281 ggctgttggc tgccaacgga cgtattggcg gatcggcaac cttcaatggg cgtgaaatcc
     5341 tcaatttgcc agagcgtgaa ctcaataaac tgcgagctga acaaatctca atgatttttc
     5401 aggacccaat gacttcgttg aatccctata tgcgcgtcgg tgaacagttg atggaagtgc
     5461 tgatgctgca taagaacatg agcaaagctg aggcgtttga agaatcggtg cggatgctcg
     5521 atgcggtaaa aatgccggaa gcgcgtaaac gcatgaaaat gtacccgcac gaattttctg
     5581 gcggcatgcg tcagcgagtc atgattgcga tggcattgct atgtcgacct aagctgctga
     5641 ttgcggatga acccactact gcgctggacg tcaccgtaca ggcgcagatc atgacgctat
     5701 tgaatgaact gaagcgggaa tttaataccg ccatcattat gattacccac gatctaggcg
     5761 tggtagcggg gatctgcgac aaagtgctgg taatgtacgc cgggcgcacg atggaatatg
     5821 gcaacgcgcg cgatgtcttt tatcaacccg tacatcctta ttctatcggt ttgctcaacg
     5881 cggtgccgcg tctcgatgcg gaaggtgaaa caatgttgac tattccgggt aatccgccaa
     5941 acctgctgcg attaccgaaa ggttgcccgt tccagccacg ttgtccgcat gcgatggaca
     6001 tttgtagtag cgcaccgccg ctggaagagt ttacgcctgg ccgtctgcgt gcttgcttta
     6061 aaccggtgga ggaactgtta tgaatgctgt aaccgaagga agaaaagtcc tcctcgaaat
     6121 cgccgatctg aaagtgcact ttgaaatcaa agatggcaaa cagtggttct ggcaaccgcc
     6181 gaaaacgctc aaagccgtcg atggtgtaac tcttcgcctg tatgaagggg aaacattagg
     6241 tgtggtaggg gaatcgggat gcggtaagtc cacctttgct cgcgccatca ttggcctggt
     6301 taaagcgacc gacggtcatg ttgcctggtt aggtaaagag ttgctgggca tgaagcccga
     6361 tgaatggcgt gccgtacgca gtgatatcca gatgattttc caggatccgt tggcatcgct
     6421 gaacccgcgt atgaccatcg gcgagatcat cgctgaacca ctgcgtactt atcatccgaa
     6481 aatgtcacgc caggaagttc gcgagcgcgt gaaggcgatg atgctgaaag tcggtttgtt
     6541 gcctaacctg attaaccgct atccgcatga gttctccggt gggcagtgcc agcgtatcgg
     6601 gattgctcgt gctcttattc ttgaaccgaa gctgattatc tgtgatgagc cggtgtcggc
     6661 gctggacgtg tcaattcagg cgcaggtggt caacctgctc cagcagttgc aacgtgagat
     6721 gggattgtca ttaattttta tcgctcacga cttggccgtg gtaaaacaca tttccgatcg
     6781 tgtgctggtg atgtatctcg gccatgcggt agaactgggg acctatgatg aggtctacca
     6841 caatccgcta catccttaca ccagggcatt gatgtcggca gtccccatac ctgatccgga
     6901 tctggagaag aacaaaacca tccagttact ggaaggggaa ttaccgtcgc cgatcaaccc
     6961 gccttccggt tgtgttttcc gtacccgttg tccgattgcc ggtccggagt gcgccaaaac
     7021 acgtcctgtg ctggaaggga gtttcagaca cgccgtttct tgcctgaaag tcgatccgct
     7081 ttaaaagcaa taagggctga cagttgtcag cccttattca cgtcaaaagc gattatttat
     7141 tcccgccaga tgatatggca aagtttgtga tctttttcgc ggcataacag aatgcgcgcg
     7201 aaaacatcgt tgatttcacc atcttcactg tccgccagac caatcaccac ttcggcaaaa
     7261 aagtccgggt tcaaatcgaa atcaacatgt tcctgccagt cttccgccgg atcgaataac
     7321 tccgcgccgc cgcgctcttc aaactgaaga ttgaacagca gaacatcagc tggatcgaga
     7381 ttgtcggcag ccagttcgag aaaaatatcg taggcctgct caagcgtttc atcttcagtc
     7441 aggcgattgt ttagatccat atccatagtc actacctgtt taacctctgt tggcgacgtt
     7501 ttacagcaac gggctgaaga agtaaaacag tcgctcggcg acacgttgcc ataatggacg
     7561 ttttagccat aaacgggcat cgagcagacg agaacgcgaa atataatcgt cctgaacggc
     7621 ggcgaggtct gcaccaaaac ctttatcgtc gattgccagg gtaatctcga aatttagcca
     7681 cagactacgc atatcaaggt taacggtgcc aaccagactt agttcaccat cgaccagcac
     7741 gctcttggta tgcagtaacc cgccttcaaa ctgataaatt ttaaccccag cagccagcag
     7801 ttctgtaaag aatgcgcgac tggcccagcc gaccagcatc gagtcatttt ttcgcggaag
     7861 gataatactg acatccaccc cgcgctgcgc cgccgtgcaa atcgcatgaa gtaaatcatc
     7921 gcttggcaca aaatagggcg ttgtcatgat caaatattca cgagccgaat aagcagcagt
     7981 caataatgcc tggtgaatga gatcttccgg aaagccgggg ccagaagcaa ttgtgtgaat
     8041 agtgtgaccg ctggcctgct caaacggcat aatattgaca tctggtggcg gcggcagaat
     8101 acgtttcccg gtttcaatct cccagtcgca ggaataaata atccccatcg cggtggcgat
     8161 ggggccttcc atacgcgcca tcagatcaat ccattgccct acgcccgcat cttgtttgaa
     8221 gtagcgaggg tcgaccatat tcatgctgcc ggtgtacgcg atgtaattat caatcatgat
     8281 catcttgcga tgttggcgca ggtccatacg gcgtaaaaac acacgcatca gattgacctt
     8341 taaggcttcg acaacttcaa taccagcatt acgcattagc tcgggccacg ggctgcggaa
     8401 aaaagccaca ctcccggcgg agtcgagcat caatcggcaa tgaataccac gccgtgcagc
     8461 ggccattaat gattcagcca cctggtccgc catgccgccg ggctgccaga tataaaacac
     8521 catctcaata ttatggcgcg cgagctggat gtcgcggatt aacgcctgca tcacatcatc
     8581 tgactcggtc atcagttgta gctgattccc tttgacccca gcgatcccct gacgacgctc
     8641 gcaaagcttg aataatggcg cagcgacact gctattttct tcggcgaaga tatgcttaca
     8701 ggctttaagg tcgttaagcc attttgcggt ggaaggccac atcgctctgg cgcgttcagc
     8761 gcggcgtttg cctaaatgga gctcgccaac ggcaagatag gcaataattc cgactaacgg
     8821 cagaatgtaa ataatcaaca gccaggccat cgcggaggga actgcgcgtc gtttcattag
     8881 aatgcgtaaa gttacgcctg caatgagcaa ccagtatccc agaatggcca accaactcac
     8941 caacgtataa acggttgtca tagattagaa atccttttga aagcgcattg ttatgagttt
     9001 acgcatatgg attcatctgg caaataaaaa cgcgggaaaa gcactggtca gcgtcagggg
     9061 cgggggtata atgaccattc tgttgttgca tagagtagtt aacatgaagc ggagtagaac
     9121 ggaagtgggg cgctggcgca tgcagcgtca ggctagccga cgtaaatcgc gttggcttga
     9181 ggggcagtcg cgccgaaata tgcgtatcca cagcatcagg aagtgcattc taaacaaaca
     9241 gcgtaactcg ttattgtttg cgatctacaa tatctaaatg tactgggcac cattgcggtg
     9301 cccaataagt ttactcttcc gtctttaaac tctatttttg cgccgattct ttactgtcag
     9361 ctttcgtttc ttgttcatcg ttatcgctga aaatgccatg accggagttc aacaacattg
     9421 aaacgagcat atcattatta atctcttcac catttaaaac tcgcgccaga atttcgctgc
     9481 caaacagttg tggtgagaga atgatatccg gatgtaccat cttaatctta ttcaggtttt
     9541 tgctatcact gacggcgaga actgttttga catcactgct catatctttt gccgagagta
     9601 caacaaacgc gttatctgca tcgttatcac tcagcgccag aatggcccgg caacgatcga
     9661 ttcccgcttt ctttaacact gaactgtcat tactgtcacc ggggataaca tcggcgttat
     9721 cgcctaagcg ttgctcaagt tgcttgatat catcttcagg caagttgctg ataaccgtta
     9781 cgttttgtcc gcgttgattc agttgcagaa tcgtattgat ggcgagaatc gaatgtccgc
     9841 aaacaataaa atgatcttta cgatgcattg tatgattgtt tccttttaca agtttgttga
     9901 atcccccgcg gataagcgga ccaaaaattg aggtcataga tgtggcaaaa acggtaatgc
     9961 cggaaataat gaccgaaata gtgaacaatc gtgctgattc agaaacaggg acaatatcgc
    10021 catagccgac ggttgacatg gtttctatcg aaaaatagaa cgcggtcatc aaactttcta
    10081 ttcgcggatt aaaaccttcg cttaaataaa gcgcaccgta agtggagtaa aacagtaacg
    10141 tcgtgaaact aataaatgcg aaaattgtcc cggctgctgc gctactgtgg gagaagtctt
    10201 tgcgcagtat cagcaaaaac accagcgtaa aaatgcaaaa tccaatacta aatttcagcc
    10261 agggataaaa atgcagagtg taaattagcg ctatcaacaa caaaatgata ctgattgccc
    10321 atgcgagctt tgcccggaac agtaagccaa tggagttcag gacaagaaat acgccgagca
    10381 taaatagtgg ggcgtttgcc agcaaggaga gatccagctc tgaaaatgct ttgatatgga
    10441 aaatatcaag caaattgacc gacataccat agattgtttt aaaaataagc aatccattta
    10501 aaaagacggc cagcgcgaga atgtcgtgcc gtagggtcac ccataacttt gttgctgttt
    10561 gtttgaatgt agtccagtga ctcactgata cctctgtaac cacaattttg tttaattatc
    10621 tcaacgtgca cattttctga acatacatgc ggcgcgtgaa tgtgttaaac aatagtagat
    10681 gactttatcc gcagtaatca aaggttttga gtgagcaact ctgcgtcagg aaaaggcatt
    10741 ttcctgaata tgttcacat
//
LOCUS       DAZFRG010000168        10753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFRG010000168 DAZFRG010000000
VERSION     DAZFRG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.168"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..1338
                     /gene="nhaA"
                     /locus_tag="ACHKUC_003464"
     CDS             172..1338
                     /gene="nhaA"
                     /locus_tag="ACHKUC_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO6619666.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1398..2303
                     /gene="nhaR"
                     /locus_tag="ACHKUC_003465"
     CDS             1398..2303
                     /gene="nhaR"
                     /locus_tag="ACHKUC_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO6619667.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(2399..2662)
                     /gene="rpsT"
                     /locus_tag="ACHKUC_003466"
     CDS             complement(2399..2662)
                     /gene="rpsT"
                     /locus_tag="ACHKUC_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO6619668.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            2765..2983
                     /locus_tag="ACHKUC_003467"
     CDS             2765..2983
                     /locus_tag="ACHKUC_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO6619669.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            2991..3932
                     /gene="ribF"
                     /locus_tag="ACHKUC_003468"
     CDS             2991..3932
                     /gene="ribF"
                     /locus_tag="ACHKUC_003468"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO6619670.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            3975..6791
                     /gene="ileS"
                     /locus_tag="ACHKUC_003469"
     CDS             3975..6791
                     /gene="ileS"
                     /locus_tag="ACHKUC_003469"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO6619671.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKNVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            6791..7285
                     /gene="lspA"
                     /locus_tag="ACHKUC_003470"
     CDS             6791..7285
                     /gene="lspA"
                     /locus_tag="ACHKUC_003470"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO6619672.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            7393..7842
                     /gene="fkpB"
                     /locus_tag="ACHKUC_003471"
     CDS             7393..7842
                     /gene="fkpB"
                     /locus_tag="ACHKUC_003471"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6619673.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            7844..8794
                     /gene="ispH"
                     /locus_tag="ACHKUC_003472"
                     /gene_synonym="lytB"
     CDS             7844..8794
                     /gene="ispH"
                     /locus_tag="ACHKUC_003472"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO6619674.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            8860..9774
                     /gene="rihC"
                     /locus_tag="ACHKUC_003473"
     CDS             8860..9774
                     /gene="rihC"
                     /locus_tag="ACHKUC_003473"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO6619675.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            9941..>10753
                     /gene="dapB"
                     /locus_tag="ACHKUC_003474"
     CDS             9941..>10753
                     /gene="dapB"
                     /locus_tag="ACHKUC_003474"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO6619676.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLN"
BASE COUNT         2452 a         2599 c         3000 g         2702 t
ORIGIN      
        1 aggtcatcag ccgccgcttt cgggcatcaa aaactcatct ctcgctgatg gcgcaattct
       61 tttaatagat cgtaaaaagc gagctattcc tacactataa tctgatttta acgatgattc
      121 gtgcggggta aaatcgtgaa aacgatctat tcacctgaag agaaataaaa agtgaaacat
      181 ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttctcattat tgccgctgta
      241 ttagcgatga ttatggccaa cagcggtgca accagtggat ggtatcacga ctttcttgag
      301 acgccggttc agctccgggt tgggacactt gagatcaaca agaacatgct gctatggatc
      361 aatgacgctc tgatggcggt atttttcctg ttggttggtc tggaagttaa acgcgagctg
      421 atgcaaggtt cgctggccag tctgcgccag gcggcatttc ctgttattgc cgcaatcggc
      481 gggatgattg tcccggcatt gctctatctg gcttttaact atgccgatcc gattacccgc
      541 gaaggctggg caatcccggc ggcgactgac attgcctttg cacttggtgt gttggcgctg
      601 ttgggaagtc gtgttccgtt agcgctgaag atctttttga tggctctggc tattatcgac
      661 gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc gatggcctct
      721 cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc
      781 cgcacgggcg tttatattct ggttggcgtg gtgctgtgga cagcggtgtt gaaatcgggg
      841 gttcacgcaa ccctggctgg cgtcattgtc ggcttcttta ttcctttgaa agagaagcat
      901 gggcgctctc cggctaaacg tctggagcat gttttgcatc catgggtggc gtatctgatt
      961 ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggtgtcac gctggaaggt
     1021 ttgacctcca ttctgccatt agggatcatc gctggtttgc tgattggcaa gccactgggt
     1081 attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cacatctgcc agagggaacg
     1141 acttaccagc aaattatggc ggttggtatc ctgtgcggta tcggttttac tatgtctatc
     1201 tttattgcca gcctggcatt tggtagcgta gatccagaac tgattaactg ggcaaaatta
     1261 ggcatccttg tcggttcaat ttcttcggcg gtaattggat atagctggtt acgcgttcgt
     1321 ttacgtccat cagtttgaca ggacgatgta ctggggagcc ataaacggct cccttttcat
     1381 tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg tattacttct
     1441 ggcatgtcta caaagaaggt tctgtggttg gcgcagcgga ggcgctttat ttaacaccac
     1501 aaaccattac cgggcagatc cgggcgctgg aagagcgcct gcaagggaaa ctatttaagc
     1561 gtaaaggacg tggtctggaa cccagcgaac tgggagaact ggtctatcgc tatgccgata
     1621 aaatgttcac cttaagccag gaaatgctgg atatcgtcaa ctatcgcaaa gagtccaact
     1681 tattgtttga tgttggtgtg gcagatgcac tttccaaacg tctggtcagc agtgttctgg
     1741 atgccgcagt tgtggaagac gagcagatcc atctacgctg tttcgaatcg acgcacgaga
     1801 tgcttttaga gcagttgagt cagcataaac tggatatgat catctctgac tgtccgatcg
     1861 attccactca gcaggaaggg ctgttttcca tgaaaattgg cgaatgtggt gtcagtttct
     1921 ggtgcactaa cccactacca gaaaagccgt ttcctgcctg tcttgaagag cgtcgtttac
     1981 ttattccggg gcgtcgctca atgttggggc gtaaactatt aaactggttt aactcccagg
     2041 gcttgaacgt cgaaattttg ggtgagtttg atgatgctgc gttgatgaaa gcctttgggg
     2101 cgacgcataa cgctattttc gttgcacctt cgctttacgc taatgatttc tataacgatg
     2161 actcggttgt ggagataggc cgtgttgaga acgtgatgga agagtaccac gcgatttttg
     2221 ccgaaaggat gattcagcac cctgcagtac agcgtatctg caatacagac tattctgcgc
     2281 tgtttactcc agcttcaaaa taaacatttg tactttgcag gccgaatttt ggcctgcaga
     2341 tggatataaa aaaacccgcc agagcgggtt tttttacaaa gctgacaaca acaggcaatt
     2401 aagccagttt gttgatctgt gcagtcaggt tagccttatg acgtgcagct ttgtttttgt
     2461 ggatcagacc tttagcagcc tgacggtcca cgattggttg catttcgtta aatgctttct
     2521 gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac tttcttgatg aaagtacgca
     2581 tcatagagcg acggcttgcg ttgtgcttac gagccttttc agactgaatg gcgcgcttct
     2641 tagctgattt gatattagcc aaggtccaac tcccaaatgt gttctatatg gacaattcaa
     2701 aggccgagga atatgccctt ttagccttct tttgtcaatg gatttgtgca aataagcgcc
     2761 gttaatgtgc cggcactcgt tacgtagtga tggcgcagga ttctaccagc ttgcggggtg
     2821 tgaatacagc ttttccgcga gaaaaattgc agcaggcggt cagtttcttc ccatgatttg
     2881 cgccatagca atgaaaagcc acttctttct gatttcggta ctcaatcgcc ggttaacctt
     2941 gaccgctgta caaggtatac tcggacgatt ttcactgttt tgagccagac atgaagctga
     3001 tacgcggcat acataatctc agccaggccc cgcaagaagg gtgtgtgctg actattggta
     3061 atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca gggcttgcag gaagaagggc
     3121 gcaagcgcaa cttaccggtg atggtgatgc tttttgaacc tcaaccactg gaactgtttg
     3181 ctactgataa agccccggca cggctcaccc ggctgcggga aaaactacgt tatcttgcag
     3241 agtgtggtgt tgattacgtg ctgtgcgtgc gttttgacag gcgttttgcg gcgttaaccg
     3301 cgcaaaactt catcagtgat cttctggtga agcacttgcg ggtaaaattt cttgccgtag
     3361 gtgacgattt ccgctttggc gctggtcgtg aaggcgattt cttgttatta cagaaagcgg
     3421 gcatggaata cggcttcgat atcaccagca cgcaaacttt ttgcgaaggt ggcgtgcgta
     3481 tcagcagcac cgccgtgcgt caggcgcttg cggatgacaa tctggctctg gcagaaagtt
     3541 tactggggca cccgtttgct atctccgggc gtgtagtcca cggtgatgaa ttagggcgca
     3601 ctataggttt cccgacggcg aatgtaccgc tacgccgtca ggtttccccg gtgaaagggg
     3661 tttatgcggt agaagtgttg ggccttggcg aaaagccgtt acccggcgtt gcaaacatcg
     3721 gaacacgccc aacggttgcc ggtattcgcc agcaactgga agtgcatttg ttagatgttg
     3781 caatggacct ttatggtcgc catatacaag tagtgctgcg taaaaaaata cgcaatgagc
     3841 agcgatttgc atcgctggac gaactgaaag cgcagattgc gcgtgatgaa ttaaccgccc
     3901 gcgaattttt tgggctaaca aaaccggctt aagcctgtta tgtaatcaaa ccgaaatacg
     3961 gaaccgagaa tctgatgagt gactataaat caaccctgaa tttgccggaa acagggttcc
     4021 cgatgcgtgg cgatctcgcc aagcgcgaac cgggaatgct ggcgcgttgg actgatgatg
     4081 atctgtacgg catcattcgt gcagctaaaa aaggcaaaaa aaccttcatt ctgcatgatg
     4141 gccctcctta tgcgaatggc agcattcata ttggtcactc ggttaacaag attctgaaag
     4201 acattatcat taagtccaaa gggctttctg gatatgactc gccgtatgtg cctggctggg
     4261 actgtcatgg tctgccaatc gagctgaaag tagagcaaga atacggtaag ccgggggaga
     4321 aattcaccgc cgctgagttc cgcgccaagt gccgcgaata cgctgcgacc caggttgacg
     4381 gtcagcgcaa agactttatc cgtctgggcg tgctgggcga ctggtcgcac ccgtacctga
     4441 ccatggactt caaaactgaa gccaacatca tccgcgcgct gggcaaaatc atcggcaacg
     4501 gtcacctgca caaaggcgcg aagccggtgc actggtgcgt tgactgccgt tctgcactgg
     4561 cagaagcgga agttgagtat tacgacaaaa cttctccgtc catcgacgtc gctttccagg
     4621 cggtcgatca ggatgcgctt aaaacgaaat ttggcgtaag caatgttaac ggcccaattt
     4681 cgctggttat ctggaccacc acgccgtgga cgctgcctgc taaccgcgca atctccattg
     4741 cacctgattt tgattatgcg ctggtgcaaa tcgacggtca ggccgtgatc ctcgcgaaag
     4801 atctggttga aagcgtaatg cagcgtatcg gcgttagcga ttacaccatt cttggcacgg
     4861 tgaaaggtgc cgagctggaa ctgttgcgct ttacccatcc gtttatggac ttcgatgttc
     4921 cggcaattct cggcgaccac gttacgctgg atgccggtac cggtgccgtt cataccgcgc
     4981 caggccacgg tccggacgac tatgtgatcg gtcaaaaata tggtctggaa accgctaacc
     5041 cggttggccc ggacggcact tatctgccgg gtacttaccc gactctggat ggcgttaatg
     5101 tcttcaaagc gaacgatatt gtcattgcgt tgttgcagga aaaaggcgcg ctgttgcacg
     5161 ttgagaaaat gcaacacagc tatccgtgct gctggcgtca taaaacgccg atcatcttcc
     5221 gcgcgacgcc gcagtggttc gtcagcatgg atcagaaagg tctgcgtgcg cagtcactga
     5281 aagagatcaa aggcgtgcag tggatccctg actggggcca ggcgcgtatc gagtcgatgg
     5341 ttgctaaccg tcctgactgg tgtatctctc gtcagcgtac ctggggcgtg ccgatgtcac
     5401 tgttcgtgca caaagacaca gaagaactgc atccgcgtac tctcgaactg atggaagaag
     5461 tggcaaaacg cgttgaagtt gacggcattc aggcgtggtg ggatctcgat gcgaaagaga
     5521 tcctcggcga cgaagctgac cagtatgtga aagtaccgga tacgctggat gtatggtttg
     5581 actccggatc tacccactct tccgttgttg atgtgcgtcc ggaatttgcc ggtcacgcag
     5641 cggacatgta tctggaaggt tctgaccaac accgtggctg gttcatgtct tccctgatga
     5701 tctctaccgc gatgaagggc aaagcgccat atcgtcaggt actgactcac ggctttaccg
     5761 tggatggtca gggtcgcaag atgtctaaat ccatcggtaa caccgtttcg ccgcaggatg
     5821 tgatgaataa actgggtgcg gatattctgc gtctgtgggt ggcatcaacc gactacactg
     5881 gcgaaatggc cgtttctgac gagatcctga aacgtgctgc cgacagctat cgtcgtatcc
     5941 gtaacaccgc gcgcttcctg ctggcaaacc tgaacggttt tgatccggca aaagatatgg
     6001 tgaaaccgga agagatggtg gtactggatc gctgggccgt aggttgtgcg aaagcggcac
     6061 aggaagacat cctcaaggcg tacgaagcat acgatttcca cgaagtggta cagcgtctga
     6121 tgcgcttctg ctccgttgag atgggttcct tctacctcga catcatcaaa gaccgtcagt
     6181 ataccgccaa agcggacagc gtggcgcgtc gtagctgcca gactgcgctg tatcacatcg
     6241 cagaagcgct ggttcgctgg atggcaccaa tcctctcctt caccgctgat gaagtgtggg
     6301 gttacctgcc gggcgaacgt gaaaaatacg tcttcaccgg cgagtggtac gaaggcctgt
     6361 ttggtctggc agacagtgaa gcaatgaacg atgcgttctg ggacgagctg ttgaaagtgc
     6421 gtggcgaagt gaacaaagtc attgagcaag cgcgtgccga taagaacgtg ggcggctcgc
     6481 tggaagcggc agtaaccttg tatgcagaac cggaactggc ggcgaaactg accgcgctgg
     6541 gcgatgaatt acgatttgtc ctgttgacct ccggcgctac cgttgcagac tataacgacg
     6601 cacctgctga tgcccagcag agcgaagtcc tcaaagggct gaaagtcgcg ttgagtaaag
     6661 ccgaaggtga gaagtgtcct cgctgctggc actacaccca ggatgtcggc aaggtggcgg
     6721 aacacgcaga aatctgcggc cgctgtgtca gcaacgtcgc cggtgacggt gaaaaacgta
     6781 agtttgcctg atgagtcaat cgatctgttc aacagggcta cgctggctgt ggctggtggt
     6841 agtcgtgctg attatcgatc tgggcagcaa atacctgatc ctccagaact ttgctctggg
     6901 ggatacggtc ccgctgttcc cgtcgcttaa tctgcattat gcgcgtaact atggcgcggc
     6961 gtttagtttc cttgccgata gcggcggctg gcagcgttgg ttctttgccg gtattgcgat
     7021 tggtattagc gtgatcctgg tagtgatgat gtatcgctcg aaggccacgc agaagctaaa
     7081 caatatcgct tacgcgctga ttattggcgg cgcactgggc aacctgttcg accgcctgtg
     7141 gcacggcttc gttgtcgata tgatcgactt ctatgtcggc gactggcact tcgccacctt
     7201 caaccttgcc gatactgcca tttgtgtcgg tgcggcactg attgtgctgg aaggtttttt
     7261 accttctaaa gcgaaaaaac aataataaac cctgccggat gcgatgctga cgcatcttat
     7321 ccggcctaca gattgctgcg aaatcgtagg tcgcatccgg caaaaatcct caaaatataa
     7381 gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt gcacttcacg
     7441 ctaaaactcg acgatggcac caccgctgag tctacccgca acaacggtaa accggcgctg
     7501 ttccgcctgg gtgatgcttc tctttctgaa gggctggagc aacacctgct ggggctgaaa
     7561 gtgggcgata aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt gccgtcaccg
     7621 gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc agaaattggc
     7681 gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggcgtgat ccgcgaaatt
     7741 aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac cgttcatttt
     7801 gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga tcctgttggc
     7861 caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg aaaacgcgct
     7921 ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc gctacgtggt
     7981 cgatagcctg cgcgagcgtg gggctatctt tattgagcag attagcgaag taccggacgg
     8041 cgcgatcctg attttctccg cacacggtgt ttctcaggcg gtacgtaatg aagcgaaaag
     8101 ccgtgatttg acggtatttg acgccacctg tccgctggtg accaaagtgc atatggaagt
     8161 cgcccgcgcc agccgtcgtg gtgaagaatc tattctcatc ggtcacgccg ggcacccgga
     8221 agtggaaggg acgatgggcc agtacagcaa cccggaaggg ggaatgtatc tggtcgaatc
     8281 gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct ttatgaccca
     8341 aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta aacgcttccc
     8401 gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc gtcaggaagc
     8461 ggtacgcgcc ctggcagagc aggcggaagt agtactggtg gttggttcga aaaactcttc
     8521 caactccaac cgtctggcgg aactggccca gcgtatgggc aaacgcgcgt ttttgattga
     8581 cgatgcgaca gatatccagg aagagtgggt gaaagaggcg aaatgcgtcg gcgtgactgc
     8641 tggcgcatca gctccggata ttctggtaca gaacgtagtc gcacgtttac agcagcttgg
     8701 cggtggtgaa gccattccgc tggaaggccg cgaagaaaat atcgttttcg aagtgccgaa
     8761 agagctgcga gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa gttatgcgaa
     8821 aatgccggtc ttgttaccgg cattttttat ggagaaaaca tgcgtttacc tatcttcctc
     8881 gatacagacc ccggcattga cgatgccgtt gccattgccg ccgcgatttt tacccccgaa
     8941 ctcgatctgc aactgatgac cactgtcgcg ggtaatgttt cggtagagaa aacgacccgc
     9001 aacgccctgc aactgctgca tttctggaat gtggatattc cgcttgccca gggcgctgcc
     9061 gtaccactgg ttcgtgcgcc gcgcgatgcg gcatctgtac acggtgaatc gggaatggct
     9121 ggctatgact ttgttgaaca caaccgtcag ccactgggta taccggcgtt tctggcaatt
     9181 cgtgatgccc tgatgcgtgc gccagagccc gttaccctgg tggccatcgg cccgttaacc
     9241 aatattgcgc tgttactttc acaatgcccg gaatgtaagc cgtatattcg ccgtctggtg
     9301 atcatgggcg gttctgctgg tcgcggcaac tgcacaccaa acgccgagtt taatatcgct
     9361 gccgatccag aagcggcagc ctgtgtcttc cgtagcggca tcgaaatcgt catgtgtggt
     9421 ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt gccggagttg
     9481 aatcgcaccg gaaaaatgct acacgccctg tttagccact accgtagcgg cagtatgcaa
     9541 agtggactac gtatgcacga tctttgcgct atcgcctggc tggtgcgccc ggaattgttc
     9601 actctcaaac cgtgttttgt cgcggtagaa actcaaggcg aatttacctc aggcacgacg
     9661 gtagttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc attggatctg
     9721 aatgtaaaag gcttccagca gtgggtagct gaggtgctgg ctctggtgcc gtaacctgtc
     9781 acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat ccctccctgt
     9841 ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg cgctggttac
     9901 tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg caaacatccg
     9961 cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg cggcgctggc
    10021 attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt tactgggcag
    10081 cgacgccggt gagctggcgg gtgcaggaaa aacaggcgtt actgtgcaaa gcagccttga
    10141 tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag gtacgctgaa
    10201 ccatctcgct ttttgtcgcc agcatggcaa agggatggtg atcggcacta cggggtttga
    10261 cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat attgcgattg tctttgccgc
    10321 caattttagc gttggcgtta acgtcatgct taaactgctg gagaaagcag ccaaagtgat
    10381 gggggactac accgatatcg aaattattga agcacatcat agacataaag ttgatgcgcc
    10441 gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gctctggata aagatctgaa
    10501 agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgcctg gcaccattgg
    10561 ttttgccacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt ttgccgatat
    10621 tggcgagcgt ctggagatca cccataaggc atccagccgc atgacatttg ctaacggtgc
    10681 ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata tgcgagatgt
    10741 gcttgatctc aat
//
LOCUS       DAZFRG010000169        10668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFRG010000169 DAZFRG010000000
VERSION     DAZFRG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.169"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            254..469
                     /gene="essD"
                     /locus_tag="ACHKUC_003475"
     CDS             254..469
                     /gene="essD"
                     /locus_tag="ACHKUC_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6619677.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            469..663
                     /locus_tag="ACHKUC_003476"
                     /pseudo
     CDS             469..663
                     /locus_tag="ACHKUC_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            646..4056
                     /locus_tag="ACHKUC_003477"
     CDS             646..4056
                     /locus_tag="ACHKUC_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6619678.1"
                     /translation="MLGKTYKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVD
                     GLKSVLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDT
                     PITRTITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIK
                     GKTTSQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDMKQCYPN
                     TALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNM
                     AWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLT
                     TQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYS
                     FSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHR
                     AGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRT
                     LTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLK
                     LPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPA
                     VQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTY
                     RFTQLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIDLTPGYFQITATPHLA
                     VYDPTVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGQASDDASGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEW
                     KDANDKWNAMWGVKIEQTEDGRHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANG
                     NETPMFVAQGNQIFMNEVFLKYLTAPTITSGGNPPVFSLTPDGRLTAKNADISGNVNA
                     NSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRNGSYASGTITVTVYDDQAF
                     DRQIVIPPVLFRGGKHENFNSNNQQSYWYSTCKLQVLKNGQEIFQQPATDVSRVFSSV
                     IDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            4115..6175
                     /locus_tag="ACHKUC_003478"
     CDS             4115..6175
                     /locus_tag="ACHKUC_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6619679.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEGFPLSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPEA
                     LRRFEVMVEDVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPYANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGH
                     FHVVTNGKAVNEVYCEYNGVTADKNIRFGGQTNTGERHLFGHIRNFRIWHKELNDRQL
                     KEVV"
     gene            6172..6450
                     /locus_tag="ACHKUC_003479"
     CDS             6172..6450
                     /locus_tag="ACHKUC_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619680.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            6463..6753
                     /locus_tag="ACHKUC_003480"
                     /pseudo
     CDS             6463..6753
                     /locus_tag="ACHKUC_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6848..7705)
                     /gene="sitD"
                     /locus_tag="ACHKUC_003481"
     CDS             complement(6848..7705)
                     /gene="sitD"
                     /locus_tag="ACHKUC_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO6619681.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(7702..8559)
                     /gene="sitC"
                     /locus_tag="ACHKUC_003482"
     CDS             complement(7702..8559)
                     /gene="sitC"
                     /locus_tag="ACHKUC_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO6619682.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(8556..9383)
                     /gene="sitB"
                     /locus_tag="ACHKUC_003483"
     CDS             complement(8556..9383)
                     /gene="sitB"
                     /locus_tag="ACHKUC_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO6619683.1"
                     /translation="MMQSAGIVVNDVAVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(9383..10297)
                     /gene="sitA"
                     /locus_tag="ACHKUC_003484"
     CDS             complement(9383..10297)
                     /gene="sitA"
                     /locus_tag="ACHKUC_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO6619684.1"
                     /translation="MRSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         2819 a         2530 c         2881 g         2438 t
ORIGIN      
        1 ctcggtggca ttctcacaaa tatcatttag tagtttacgt acgtaaaaaa ttggttatgc
       61 tgttaagagt ggttacttcg tcacacagct taaacccgcc gtcgagcggg tttttccatt
      121 ttttgagtct cgatattagc tgataaccca atacctgagt tattcactga ctccgaatct
      181 gttacgtttc tgcctttatt gcgatacgta gtatcccctt aatttacacc cgctttgtct
      241 gcgaggtggg gttatgaaat ccatggataa gttaacaacg ggtgtcgcct atggcacctc
      301 agcaggtagt gccgggtact ggtttttaca gttgctcgat aaagtcacgc cctcacagtg
      361 ggcggcaata ggtgtgctgg gtagtctggt atttggcttg ctgacgtatc tgacaaacct
      421 ttatttcaag attaaagaag ataagcgtaa ggctgcgaga ggtgaataat gtcgccatca
      481 ttacgcaagg ctattgctgc tattggtggt ggggctgttg ccatagcgtc tgtgctcatc
      541 actggtccga gtggtgacga tggcctggaa ggtgtcagct acataccata caaagatatt
      601 gttggtgtat ggactgtatg tcacgggcat acaggaaaag acatcatgct cggtaaaacg
      661 tataaaggca gcagtaaggg gcataccccg cgcgaagcga aggacaacct gaaatccacg
      721 cagttactga gtgtgatcga tgccatcagc gaagggccga ttgaaggtcc ggtggatggg
      781 ttaaaaagcg tgctactgaa cagtacgccg gtgctggaca ctgaggggaa taccaatatc
      841 tccggtgtca cggtggtgtt ccgggcaggt gagcaggagc agactccgcc ggaggggttt
      901 gaatcctccg gctccgagac ggtgctgggt acggaagtga aatacgacac gccgatcacc
      961 cgcaccatca cgtcggcaaa catcgaccgt ctgcgtttta ccttcggtgt gcaggcactg
     1021 gtggaaacca cttcaaaggg tgaccggaat ccgtcggaag ttcgcctgct ggttcagata
     1081 cagcgtaacg gtggctgggt gacggaaaaa gacatcacca ttaagggcaa aaccacctcg
     1141 cagtatctgg cctcggtggt ggtggataac ctgcctccgc gcccgttcaa tatccggatg
     1201 cgcaggatga caccggacag caccacagac cagctgcaga acaaaacgct ctggtcgtca
     1261 tacaccgaaa tcatcgatat gaaacagtgc tacccgaaca cggcactggt cggtgtgcag
     1321 gtggactcgg agcagttcgg cagtcagcag gtgagtcgta attatcatct gcgcgggcgc
     1381 attctgcagg tgccgtcgaa ctataacccg cagacgcgac aatacagcgg tatctgggac
     1441 ggaacgttaa aaccggcata cagcaacaac atggcctggt gtctgtggga tatgctgacc
     1501 catccgcgct acggcatggg gaaacgtctt ggtgcggcgg atgtggacaa atgggcgctg
     1561 tatgtcatcg gccagaattg cgaccagtcg gtgccggatg gttttggtgg cacggagccg
     1621 cgcatcacct gtaatgcctg gctgaccaca cagcgtaagg cgtgggatgt tctcagtgat
     1681 ttctgctcgg cgatgcgctg tatgccggta tggaacgggc agacgctgac gttcgtgcag
     1741 gaccggccgt cggataaggt gtggacctat aaccgcagta atgtggtgat gccggatgat
     1801 ggcgcgccgt tccgctacag tttcagcgcc ctgaaggacc gccataatgc cgttgaggtg
     1861 aactggattg acccggataa cggctgggag acggcgacag agcttgtgga ggacacgcag
     1921 gccattgccc gttacggtcg taacgtcacg aagatggatg cctttggctg taccagtcgg
     1981 gggcaggcac accgagccgg gctgtggctg attaaaacgg aactgctgga aacgcagacc
     2041 gtggatttca gcgtgggcgc agaagggctt cgccatgtgc cgggcgatgt tattgaaatc
     2101 tgcgatgatg actatgcggg tatcagcatc ggcgggcgcg tgctggcggt gaacagccag
     2161 acccggacgc tgacgctcga ccgtgaaatc actctgccat cctccggtac cacgctgata
     2221 agcctggttg acggaagcgg caatccggtc agcgtggagg tccagtccgt caccgacggc
     2281 gtgaaggtaa aagtgagccg ggttcctgac ggcgttgccg gatacagcgt gtgggggctg
     2341 aagttgccga cgttgcgcca gcgcctgttc cgctgcgtga gtatccgtga gaacgatgac
     2401 ggcacgtatg ccatcactgc cgtgcagcat gtaccggaaa aagaggccat cgtggataac
     2461 ggggcgcact ttgacggcga ccagagcggc acggtgaatg gtgtcacgcc gccagcggtg
     2521 cagcacctga ctgccgaagt caccgcagac agcggggaat atcaggtgct ggcgcgctgg
     2581 gacacgccga aggtggtgaa gggggtgagc tttatgcttc gcctgaccgt ggcagcggat
     2641 gacggcagtg agcggctggt cagcacggcc cggacgacgg aaaccacata ccgcttcacg
     2701 caactggcgc tggggcggta cacgctgaca gtccgggcgg taaatgcgtg ggggcagcag
     2761 ggcgatccgg cgtcggtatc gttccggatt gccgcaccgg cagcaccgtc gaggattgat
     2821 ctgacgccgg gctattttca gataaccgcc acgccgcatc ttgccgttta tgacccgacg
     2881 gtacagtttg aattctggtt ctcggaaaag cggattaccg atatcaggca ggttgaaacc
     2941 acagcccgct atcttggcac ggcgctgtac tggatagctg ccagtatcaa tattaagccg
     3001 ggccatgatt attattttta tatccgcagt gtgaatactg ttggcaaatc ggcattcgtg
     3061 gaggctgtcg gtcaggcgag cgatgatgct tccggctatc tggatttttt caaaggccag
     3121 ataactgaat cccatctcgg caaggagctg ctggaaaaag tcgagctgac ggaggataac
     3181 gccagcaaac tggaggagtt ttcgaaagag tggaaagacg ccaacgataa atggaatgcc
     3241 atgtggggcg tcaaaattga gcagaccgaa gacggcaggc attatgtcgc gggtattggc
     3301 ctcagcatgg aggatacgga ggaaggcaaa ctgagccagt tcctggttgc cgctaaccgt
     3361 atcgcgttta ttgacccggc aaacgggaat gaaacgccga tgtttgtggc gcagggcaac
     3421 cagatattca tgaacgaagt gttcctgaag tatctgacgg ctcccaccat taccagcggc
     3481 ggcaatccgc cggtattttc cctgacaccg gacgggcggt tgacggcgaa aaatgccgat
     3541 atcagcggta acgtgaatgc gaactcaggg acgctcaata atgtcacgat taaccagaac
     3601 tgtcggattc tgggaaaact gtctgccaac cagattgaag gtgatattgt caaaacggtg
     3661 ggaaaagcct ttccgagaaa tggcagttat gccagcggta caataacggt cactgtgtac
     3721 gatgaccagg cttttgaccg tcagatagta atcccacccg ttctgtttcg cggtggtaag
     3781 catgaaaact tcaacagcaa caaccaacag tcatactggt attcaacctg taagctgcag
     3841 gtactgaaga acggacagga aatctttcag caacccgcga cggatgtcag cagggtattt
     3901 tcatccgtca ttgatatgcc agcgggacac ggccatgtca ccctgacttt caatgtttct
     3961 tcatatggtg caaataactg gacgccaacg accagtatca gcgaccttct tgttgtcgtg
     4021 atgaagaaat caacagccgg tatcagtatc agttgaattt tataacccaa atacgggcgc
     4081 cagaaatggc gcctttttta tttgtggagt gaatatggca gtacagattt caggcgtgct
     4141 gaaagacggt gcaggaaaac caatacagaa ctgtactatt cagctcaaag caaagcgtaa
     4201 cagcaccacg gtactggtga acacggtggc ctctgaaaat ccggatgaag ccgggcgtta
     4261 cagcatggat gttgagtatg gccagtacag cgtcaccctg ctggttgaag gttttccgct
     4321 ttcacatgcc gggaccatta ccgtctatga aggttccaga ccaggtacgc tgaatgattt
     4381 tctcggtgcc atgacggaag atgatgtcat gccggaggca ttgcgtcgtt ttgaggtaat
     4441 ggtggaggat gtggcacgca acgctgaagc cgcctctcag agcgcagcgg cagcaaagaa
     4501 atcagaaaca gcagcggcat cgtccaggaa cgcggcgaaa acatcagaga cgaatgcagg
     4561 taacagcgcg aaagcggcag cttcttcaaa aacagccgca caaaacgcag caacagcggc
     4621 agaacgttca gagacaaatg cccgtgcgtc agaagaagcc tccgcagaca gtgaagaggc
     4681 ttcccgccgt aatgcagagt cagccgctga aaatgccgga gtcgccacca caaaagcgcg
     4741 ggaggctgca gcagacgcaa caaaggccgg gcagaaaaag gatgaggctc tgtcggcagc
     4801 gacacgagct gaaaaggcgg cagaccgcgc agaagccgca gcggaagtga ctgcagagcc
     4861 ctatgcgaat atagtgccgc cgctgcctga tgtgtggata ccgtttaacg attcactgga
     4921 tatgattgcg ggtttttctc cgggctataa aaaaatagct attggtgacg atgtggttca
     4981 ggtcgccagt gataaacagg ttaatttcag tcgcgcatca acggcaacat atatcaacaa
     5041 atctggcgaa ctgaaaacgg cggaaattaa tgagccgcga tttgagtgtg atggcctgct
     5101 tattgaggga caaagaacga actacatgct caattcggaa agtccagcca gctgggggaa
     5161 gtcatcaaac atggatgtgc ccgaaaccgg gacggatagt tttggtttta cttatggaaa
     5221 gtttgtctgc aacgattctc tggttgggca aacttcggct attaatatgg catcaattgc
     5281 tgcaacaaag tcagttgatg tctcaggcga taacaagtac gtgacaacct catgccgttt
     5341 taaaacagaa cgacaggtaa ggttacgtat acggtttgat aagtatgatg gtagtgcaac
     5401 aacttttctt ggcgatgcgt acattgatac gcaaacgctt gaaattaata tgacaggtgg
     5461 tgctgccggc agaattacgg cacgagtcag gaaggataag accacgggct ggatttttgc
     5521 agaggcaacg attcaggcaa ttgatggtga gttaaaaata ggctctcaga tacagtattc
     5581 tcctgagcag ggtggggcaa cagtatctgg tgactatatt tatcttgcca ccccacaagt
     5641 agagaatggg ccgtgtgtat catcatttat tatttcagga ggcagcgcaa cgacaagagc
     5701 cagtgatttg gttagtatcc ccaccagaaa taatctttat aagttaccat ttactttttt
     5761 acttgagatt cataaaaact gggatattgc accaaacgcc gcaccccgcg tgtgggatat
     5821 agcagcagcc aataccgggc aatcagcaat tgcagcaatc aacagaggta gtggtaagtt
     5881 atatatgagt ctgtcaaacc cttcaggctc gtatgttaat agcgcagcga cagatgtatt
     5941 tgcagagaaa accacatttg gatgtattgc aaaagctgat ggtcactttc atgtggtgac
     6001 aaatggtaaa gcggttaatg aagtttattg tgaatataat ggcgtgaccg ctgataaaaa
     6061 tatccgattt ggagggcaga cgaatactgg agaacgacat ctgtttggcc atattcgcaa
     6121 tttccgcata tggcataaag aattaaatga caggcaatta aaagaggtcg tatgaaagat
     6181 ttaactttga agtttcatga caaactgcag tttaaggcct tcctgtcatc tcttggctgg
     6241 gcggaagatg aagacctcca gaataaactg ttagttgatg aaattggttt cacctacaca
     6301 gaaacagggg taacagaaga gggagaacct gtctgtatcc ggaatgatgg ttattttgtc
     6361 aacattcgca ttcttgatga cttgtttgat gtttctgtat tctctgatta tgtcgtggag
     6421 ctggaaacac cgcttcggga atggagctga aaggaggaaa taatggatat aagcccctta
     6481 cttcatgcac tttgtgctgt ggctgcgcag atactggttg gtctttttac cggaaactgg
     6541 gcttacggag cgatagccgg ttgtacgttc ttcattgcgc gtgaacatac ccaggcagaa
     6601 tatcgctgga ttgaaatgtt cgggcatggc aagcgaatga atatgccgtg gtggggcggt
     6661 tttgatccgc gtgcttggga tgtggcaagc ctgatggatt ttgctgtgcc ggtggtggcg
     6721 tgtctgctgg tctggctgtt ggttaatcgt gggcgaaaaa aggtgagctg tatatgcaac
     6781 ggaggaagaa accccgttgc tggaagccag gaaaaagtat cgggtgttgc tgtcctcgga
     6841 tgttgtgcta attgggtgat aacagatcct gtgtatggga gtttcccttt ttgcgtgcgt
     6901 tgatgctggt aacggcaaac gtcatgatga acaaaaccgc gaataagacg acaatcgtcg
     6961 gagctggtgc gctgtcgata aaaaaggaca accagacacc agtcattgac actattaccg
     7021 atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt accgcaatgg
     7081 cgccaggcgc aatgagcaga gaaatggaga gaatgatacc taccgctttc agcgtcgcca
     7141 caatggtcag agagaccatg cacagcagcc cataatgcag ccatctcgta tgcaaaccgc
     7201 ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat tttacgctaa
     7261 taacaagtgt aaccagcccg gcaataatca tcgtctggat tatatcgccg atggttatcc
     7321 cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttgact gcaatataca
     7381 aaatcagacc tgcggcaaac atgccagaaa aaacaatccc catcactgta tcttgcttga
     7441 ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg gcgacgaatg
     7501 cccctgtagc caaaggtaac cccagaatcc aggcaagcac gatgccagga aaaaccgcat
     7561 gactcatcgc gtcacccatc agtgcccagc ctttcaggac cagaaagacc gacagcagcg
     7621 cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag gtgaactgca
     7681 gtggctccag taataatgcc atcatgagcg atcctccagc gccttatgcg cacgccgacg
     7741 atttgccaaa agcccatggg tgggcgcaaa gacaaatgct aaaagaaaca gcagtgtctg
     7801 cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc acgcgccgag
     7861 gaaactggtt acactgccaa tagttacagc aatcatgagc aggcggggaa aacgatcggt
     7921 taacaaccat gctgttgctc cgggagtcac caccagacag atcaccagaa aggcaccaac
     7981 cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga gttttaatct
     8041 gcccggatgc aatccaatag cacgggcgtg attctcgtcg aaaaaggtca ccataagatc
     8101 tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga gttgcaggat
     8161 atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt tcacagatgt
     8221 cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga tcaggccgat
     8281 gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc ccgcagccag
     8341 tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg cccccgcaac
     8401 acccgggaca atagagtgcg acaacgcatc gccaatcagc gaccagcctt ttagcatcag
     8461 atagcatgac aggaaagcgc agagaccgcc gaccatcgcc gagacccaca tcgcattgag
     8521 catatattca tagctgaagg gttccagtag cacgttcatc tttcttccct ctgcaccgct
     8581 gacggtcgat gagccacaaa agggcgttca tcgtcagtaa taatgctttc ttccgagccg
     8641 ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc ggctgtaaaa
     8701 gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt ataatcgcaa
     8761 aatgtcgtga cggatccgag attgtgcgtt gagacaagca tcgttttgcc ttccgcgcgt
     8821 aactccctca acaggctgat gattttggct tcggttttca catcaacgcc agtaaaaggt
     8881 tcatccagca ggatcacatc accctgctgt gcaatcgctc tcgccagaaa gacgcgtttc
     8941 ttttgtccac cggaaagctc gccgatttgt cgatggcgaa aattcaccat atcgacacgt
     9001 tccagcgcat cggtgacaat ttgacgatcc ctctttttgg cgatacgcaa catacccata
     9061 tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga ccagtcaacc
     9121 tcttctgact gagggacgta ggcaaccagg tttttctgta acgcctgtcg cgtgggaata
     9181 cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc tttaaacaac
     9241 gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact tggtactgtg
     9301 aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagcaac atcattaacg
     9361 acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt ccctgtacca
     9421 gcgtactggt agtaacctta agaaggtcga tatacgtcgg taccgggccg ttttctgtgc
     9481 tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc gcaacctgac
     9541 gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga tttttcttaa
     9601 ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca ttaatcggcc
     9661 acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc ccttcactgg
     9721 tgaccatcca tcgctgattc tcaggaagtt ccgtaatctg cttacgcagg ggggcaaggg
     9781 tttgggtaat cttggcttta taagtatcgg cattgcgttg gtaggtttgt gcatttgccg
     9841 gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca ttatctggcg
     9901 acatccatgc atgggggtta ggtttgccct catagggtcc ttcggtgatc cctacaggcg
     9961 tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa aagcgttgga
    10021 accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc gcacgtttaa
    10081 tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt atggatgaga
    10141 cttctgcagc atctcccgcc acgtttttgg ccatatccgc gatgatggta aatgttgtaa
    10201 tgaccttaaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg agtgcgaggc
    10261 cccccaagag catggttact ttttttattg agcgcataat ttagtaccta tagtgagcaa
    10321 aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacag aatgaaaatg
    10381 attatcatta acatatttga ggcccaaaac agggattagt ttgtttcctg ctggatgaaa
    10441 ggactcttgg gatagcacat gccgcacgaa gagcagcatt acatgatgtt ttgtcgaatg
    10501 agctacgaat cctgatagaa atacgaaaca agctagcaca tggacaatgg atctatccct
    10561 tcaataatga aggaaatact gtggaccctg ataaatataa attaatcaat aaagaaaact
    10621 tgcagtcact ccaattcaaa tattcattag cacagcattt agttgacg
//
LOCUS       DAZFRG010000170        10640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFRG010000170 DAZFRG010000000
VERSION     DAZFRG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.170"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..720
                     /locus_tag="ACHKUC_003485"
     CDS             91..720
                     /locus_tag="ACHKUC_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO6619685.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     CDDVNVAVIDIAMPDENGLSLVKRLRAQKPQFRAIILSIYDTPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGMGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALNHHLLAGH"
     gene            720..2261
                     /locus_tag="ACHKUC_003486"
     CDS             720..2261
                     /locus_tag="ACHKUC_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6619686.1"
                     /translation="MSRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLP
                     HGVYLAFLILHSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLLPAIIAQNV
                     WHRFPLYWQRLTLLLASVTAASLLNTAMLTPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLRLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKSLARELHDEIGQNITAI
                     QIQSQLVTRARDPAQVHSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSELGIHCQFTYQLTTTPENETVRFTLYRLLQELLNNVCKHAGASEVTIILRQ
                     QGEILHLEVSDNGVGIASGKMTGFGIQGMRERVSALGGDLMLEKHNGTRVIVNLPTFL
                     QQIAG"
     gene            2334..3650
                     /gene="uhpC"
                     /locus_tag="ACHKUC_003487"
     CDS             2334..3650
                     /gene="uhpC"
                     /locus_tag="ACHKUC_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6619687.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGIGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKSTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            3647..4678
                     /locus_tag="ACHKUC_003488"
     CDS             3647..4678
                     /locus_tag="ACHKUC_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6619688.1"
                     /translation="MKMPLTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCETET
                     KEFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTERLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDNYGSTDVRKALINK
                     WVSEVKMGK"
     gene            4747..6825
                     /locus_tag="ACHKUC_003489"
     CDS             4747..6825
                     /locus_tag="ACHKUC_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6619689.1"
                     /translation="MSQTLALHPAKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYSWSHFNISWLWYLLPSLLLVRPFNEAKREQRSRHYFDAGWALLCIAFMVIS
                     ATIVGRGLGYAAIVLFIELSAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFANDAGEFAPLAFMNVLSQAHIIQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAVIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFSGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVCSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKSIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            6837..7883
                     /gene="fbpC"
                     /locus_tag="ACHKUC_003490"
     CDS             6837..7883
                     /gene="fbpC"
                     /locus_tag="ACHKUC_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO6619690.1"
                     /translation="MSQKNFVELRNVTKRFGSNMVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRNEVKQRVKDALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQELYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAYFPVQGRGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDTGEHYYL
                     EIHPYGMFVLADSA"
     gene            8152..8390
                     /locus_tag="ACHKUC_003491"
                     /pseudo
     CDS             8152..8390
                     /locus_tag="ACHKUC_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            8689..8877
                     /locus_tag="ACHKUC_003492"
     CDS             8689..8877
                     /locus_tag="ACHKUC_003492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619691.1"
                     /translation="MTNEELQAIALSMFEHQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPS"
     gene            9205..9399
                     /locus_tag="ACHKUC_003493"
     CDS             9205..9399
                     /locus_tag="ACHKUC_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619692.1"
                     /translation="MNENRIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRPDKKHFLECNEDK"
     gene            9760..9888
                     /locus_tag="ACHKUC_003494"
     CDS             9760..9888
                     /locus_tag="ACHKUC_003494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619693.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(10199..10535)
                     /locus_tag="ACHKUC_003495"
                     /pseudo
     CDS             complement(10199..10535)
                     /locus_tag="ACHKUC_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2532 a         2683 c         2677 g         2748 t
ORIGIN      
        1 gttacatcca ttccatatct gtcgctggtt gattgccagc gacgcgtcgt gatgcatgat
       61 aggagaatga gataataaga gaagaaaagt atgattcggg ttgtgctggt ggatgaccat
      121 gtggtggtgc gctctggttt cgcacagtta ctctctctgg aagacgatct ggaggtcatt
      181 ggtcagtaca gtagtgccgc acaggcatgg tcggcattaa tctgtgatga cgtcaatgtt
      241 gccgtcattg atattgctat gccggacgaa aacggcctca gtttggttaa gcgcctgcgt
      301 gcacaaaaac cgcaattccg cgccattatt cttagcattt atgacacgcc aacctttgta
      361 caaagcgctc tggatgctgg tgccagtggc tatttgacca aacgctgtgg gccggaagag
      421 ttagtgcagg cggtgcgctc tgtcggtatg ggagggcatt atctctgcgc cgatgcaatt
      481 cgggctttac gcggcggcgg gcaacccgcc caggcactgg aaattctcac cccacgagaa
      541 cgtgaagtgt ttgatttatt agtcaaaggc gacagtgtaa aagagattgc ctttaagctc
      601 gaactcagcc ataaaacggt acatgtacat cgcgccaacg tgctgggaaa actgaactgt
      661 catagcacca ttgagctggt gcattttgca ctcaaccacc atctgctggc ggggcattaa
      721 tgtcgcgtaa ccttcgtcat tgggtgattt cgctgtttat tgtgctggcc tggggaagcg
      781 gctggctgat gttatggacg ctcagctttt atctgaccaa taacggtcaa caggcagtgt
      841 tatttctgcc gcacggagtt tacctcgctt ttcttatttt gcactctcgc cgttactggc
      901 ctgcgttggt gctcccccct ttgctgatga tgttctggct gcacagcgag caactgttga
      961 acggctatct catgctggca acgccggtta ttagcctgct tcctgccatc attgcacaaa
     1021 acgtctggca ccgttttcca ctttactggc agcgtctgac gttgctgtta gcctcagtaa
     1081 cagcagcttc attgctgaat acagccatgc taaccccttt tatgtcaggc ccaataatgc
     1141 tgcccggtct gacctcattt accggcggcg tattgttgac gccgtttgtc tatcttatct
     1201 ttgagtttct gcgccagcag catcgctatc agcttctgag actcgatacg cataatcccc
     1261 ccttgcgaac ctctttaatc atctggtgta gcctattttt tattattggt atcggcaccc
     1321 agatggtgtt gtcgcctgaa atcgaacgcc tgctgctgat cgtggtattt ctacccaatg
     1381 tagtcatggc ctggaaattt ggctggcaag ggggcgtact ctcggggctt ttgggcagca
     1441 tgatgatcac tattgcccgc cagattggtg tgggctttag taacctggtg gaactggaga
     1501 tttttctcgc aacccaagca ttgctgggaa ttggcctggg cattgccatt agtcggcagc
     1561 aacatctggc gctaaacctg catcattacc gtcagcgact ggaggcagaa ctggcagcac
     1621 ggcgggcatt ggctgaaaag ctgatccata ccgaggaaga cacgcgtaaa agcctggctc
     1681 gcgagctgca cgacgaaatc ggtcagaaca ttaccgccat tcagatccag tcgcaactgg
     1741 tgacgcgggc acgcgatccg gcgcaagttc attccgcagc cagccaaatc aacgaactcg
     1801 cccgccgcat tcatctctcc acccgccagt tgctgcgcca gttgcggccc cccgcgcttg
     1861 atgaactgac attccgcgaa gcattacttc atctgattaa tgaatttgcc tttagtgaac
     1921 tcggcattca ttgccagttt acctatcaac taaccaccac gccagaaaac gaaacggttc
     1981 gctttacact gtatcggtta ttgcaggagt tactcaataa tgtctgtaaa cacgccggag
     2041 ccagcgaagt gacgattatt ctgcgccagc agggagagat attgcaccta gaggtcagcg
     2101 acaacggtgt ggggatcgcc agcgggaaaa tgaccggatt tggcattcag gggatgcgtg
     2161 aacgcgtcag tgccctgggc ggcgacttaa tgctcgaaaa acataacggt acgcgggtaa
     2221 ttgttaacct gcccacattt ttgcaacaaa tcgccggata accaggaaaa agtcttagtc
     2281 atcccggatt ttctctcatc ctctttcact ttcattttca tatagtcagc aacatggaga
     2341 cgaccatgca cgcgcgttca gcgagagaga ttaatcaatg ctaccggaca ttacgtccac
     2401 gattgctgat gtgcatgatt attggctacg ccgcatttta tctgacgcga aaaagcgtga
     2461 attacgtcct tccggcgctg caaaccgacc tcggactgga taaaggcgac attggcctgc
     2521 tgggttcact gttttatctc agctacggat tgtcgaaatt taccgctgga ctgtggcatg
     2581 acagccacgg acaacgcggt tttatgggta tcggcttgtt cgccactggg ctattgaacg
     2641 tggtgtttgc ctttggcgaa tcacttacgc ttctgcttgt ggtctggaca ctgaacggat
     2701 tttttcaagg ctgggggtgg ccaccgtgtg cccgcttact gacccattgg tattcccgta
     2761 atgaacgtgg tttctggtgg ggctgctgga atatgtcgat caacattggc ggagcgatta
     2821 ttccgctgat tagcgccttt gccgcccact ggtggggctg gcaggccgcg atgctgacac
     2881 cggggataat cagcatggca ttagggatct ggttaacgct acaactcaaa agcacgcccc
     2941 aggaagaggg attaccgaca gttggtcact ggcgtcacga tccgctggag ttacgtcagg
     3001 aacaacaaag tccgccgatg gggctgtggc aaatgttgcg caccaccatg ctgcaaaatc
     3061 cgctgatctg gctactgggt gtctcttatg tacttgttta tgtgatccgg attgcgctaa
     3121 acgactgggg caatatctgg ctgacggaaa gccacggcgt caacctgctc agcgccaacg
     3181 ccacggtaat gctgtttgaa gtaggcggct tactcggcgc gttatttgcc ggatggggtt
     3241 cggatttgct gtttagcggg cagcgagccc ccatgatttt gctgtttacc cttgggttga
     3301 tggtttcggt cgctgcactc tggttggcac cggttcatca ctatgccctt ctggctgtgt
     3361 gcttttttac tgtgggcttt tttgtctttg gcccgcaaat gctgattgga cttgcggcag
     3421 tggaatgtgg acacaaagct gcggcgggtt caatcaccgg atttctcggc ctgttcgcct
     3481 atctcggtgc ggcgctggca ggctggccgt tgtcgctggt tatcgaacgc tacggctggt
     3541 cgggaatgtt cagtttactc tcggtcgccg cagtacttat gggtttactg ctgatgccgt
     3601 tactgatggc gggcatcacc accactcacg ctcgaaggat aaaacaatga aaatgcccct
     3661 tacctctact ctgattgctt ctgccgttgc gctggcaacc ttaacgggtg ccgcgcaggc
     3721 aaaaggccgg ctggtggtct attgcagcgc cactaatgaa atgtgtgaga ccgaaaccaa
     3781 agagtttggc gagaaatatg atgtgaaaac ctcgtttatc cgcaacggct ccggtagcac
     3841 cctggcaaaa gtcgatgctg aaaagaaaaa cccacaggct gacgtctggt acggcggcac
     3901 tttagacccg cagtcgcagg ccggcgagat gggcctgtta caggcatata aatcgcctaa
     3961 ccttgaacag atcatggaaa aattccgcga tccggcaaaa gtgaaaggaa acctttcctc
     4021 cgccgtttat gtggggatcc tcgggtttgg cgtcaacaca gagcgtctga aagagaaaaa
     4081 cctgcctgtg ccgaagtgct ggaaagatct caccaaacct gaatataaag gcgaaattca
     4141 gattgccgat ccacaaagtt ccggtaccgc ctacaccgcg ctggctacct ttgcgcagct
     4201 gtggggggaa gatcaggcct ttgattatct aaaacaactt aacgccaacg tctcccagta
     4261 caccaaatca ggcattgccc cggcacgtaa cgccgcccgc ggcgaaacgg cgattggtat
     4321 tggtttcctg catgactact cgctggaaaa agaacagggc gcaccactgg agttgatttc
     4381 cccgtgtgaa ggtacgggct acgaaatagg cggcgtcagc attctgaaag gtgcgcgcaa
     4441 cctcgacaac gccaaactgt tcgtggactg ggtattatca aaagaagctc aagaactggc
     4501 gtggaagaaa gggaaatctt atcagatcct gaccaatacc accgccgaaa cgtcgccaaa
     4561 ttcgctgaag ctcgacgacc tgaaattaat caactacgac atggataact acggttcaac
     4621 tgatgttcgt aaggcattaa ttaataaatg ggtcagcgaa gtgaagatgg gtaaataaac
     4681 ccgtattgtg tgcacttgcc gggtgcagca cagacgcctg ccccggccaa ccataccgga
     4741 actgctatgt cacagacact tgcactccat cccgcgaaaa agcgggatgc gattttcctt
     4801 tgggttttgc tgggctggct ggcatttatc ctgttgccga gctggagcct cgattatggt
     4861 ctgctggagt ccacgagtga tgagatcctc gaggcgtaca gctggtcgca ttttaatatc
     4921 agctggctgt ggtatttact cccgagtttg ctgctggttc gcccgtttaa tgaggccaaa
     4981 cgcgagcagc gtagccgtca ttattttgat gccggctggg cattgctttg tatagcgttt
     5041 atggtcatca gtgccaccat cgttggccgc ggtctaggct acgccgccat cgtgctgttt
     5101 atcgaattaa gcgcgataat gacgctggcc ttaacccgcc tcgaatggct gggtggcgac
     5161 cgctttgtga tcggctcgct gattgcgatt gtggcgctga ttggcatttt tatcgtctgg
     5221 ccgagcatcg ccatttttat cccgatgttc gccaacgacg cgggagagtt cgccccactg
     5281 gcgtttatga atgtgctctc gcaggcccat atcattcagg tgatcgtcaa ctctatcgtc
     5341 ttatcgatag cggtgggtat tggctgcact ttcttcggtc tggtgctggc gatatatacc
     5401 acgcgcatcg ctaaacgcag cgccgtaatc ggccgcatct tttccattct gccaattgtc
     5461 acgccaccgt ttgtggtcgg cttaggcgtt acgctcatga tggggcgctc cggctacata
     5521 acggaattta tggtggagtg gtttgggctg accaacacca actggctgta tggttttacc
     5581 gggatctggc tggcacaggt cctggcattt acgccaatgg cttttatgat cctcgatggg
     5641 gccattaaaa ccattcatcc ctcgctggaa gaagcctcct acaccctacg cgccagccgc
     5701 tggcaaacct ttagcggtgt gtttgtgccg ttgttaaagc ccgcgcttgc caatgccttt
     5761 ttaatcgtcg tggtgcagtc actggctgac ttcagtaacc cgctggtgct tggcggcaac
     5821 ttcgacgtac tggcgacgca aatttacttc tacattaccg gctcgcagct ggattatcag
     5881 gccgccagca cccttggcgc tttcctgctg ctgttctcgc tactggtgtt ctgcgtccag
     5941 tatatgtgga ttggtaaacg ctcttacgtc acggtttccg gaaagtccta ccgcggcgat
     6001 gtccagccac taccggtcac cctggtgtgt agcgtggtcg ccatacttgc gatatggatt
     6061 gcctttaacg ccctgctcta tggcagcatt ttctacggca gcttcaccgt gaactggggc
     6121 gtggactaca ccctaacgct ggataacttt atcaagttat ttggtcaggg gatgagcgac
     6181 ggcgcgtggc cttcactgtt agataccttg ctctacgccg ggattgccgc acccattacc
     6241 gctatttttg gcctgctcat cgcctgggtg gtggtgcgcc aacaattcaa aggcaaaaag
     6301 agcatcgagt tcaccaccat gttgtgcttt gccgtaccgg gtacagtggc gggtgtctcg
     6361 tatattcttg cctttaatag cgccccagtc tatctcaccg gaacggcagc aattgtgatt
     6421 atctcgatgg tgatgcgtaa cgttccggtg ggtattcgcg ccgggattgc aggtctgggg
     6481 cagatcgaca aatcgctgga tgaagcctca cttagcctgc gtgcaggttc cctgcgaacc
     6541 attacgcaca ttttactgcc gttattacgc ccagctattc tctcggccct gatttacagc
     6601 tttgtgcggg ctatcacgac ggtcagcgcc atcgtctttc tcgtcacgcc ggacacccgc
     6661 gtggcaaccg cctacatcct taaccgcgta gaagacggcg aatatggcgt agccatcgcc
     6721 tacggttcga ttctgatcgt ggtgatgctg gcgattattt tcctctttga ctggctgatc
     6781 ggtgaagcac gtatttcccg ttctaaagcc aaaaaccagg cgtaatggag cgcactatga
     6841 gtcagaaaaa ttttgtggaa ctgcgcaacg tcactaaacg attcggcagt aatatggtta
     6901 tcgacaatat caatctcacc atcccacaag ggcaaatggt gacgctgctc ggtccttccg
     6961 gatgcggcaa aaccaccatt ctgcgtctgg ttgccgggct ggaaaaaccg agcgaagggc
     7021 aaatttttat agatggcgaa gatgtcacgc atcgttccat tcagcaacgc gatatctgca
     7081 tggtgtttca gtcatacgct ctgttcccac atatgtcgct gggcgaaaac gttggctatg
     7141 ggctgaaaat gcttggcgtg tcgcgtaatg aagtgaagca gcgggtgaaa gatgcgctgg
     7201 caatggtgga tctggaagga ttcgaagacc gctatgtcga tcagatttcc ggcggtcagc
     7261 aacagcgtgt ggctctggcc cgtgcgttga tcctcaagcc aaaggtgctg ctgtttgatg
     7321 agccgttaag taacctcgat gccaacctgc gccgcagtat gcgcgataag atccgcgagc
     7381 tgcaaaagca gtttaatatc acgtcgctct acgtgactca cgatcaaagt gaagctttcg
     7441 cggtgtccga cactgtactg gtgatgaata aaggtcacat catgcagatc ggttcaccgc
     7501 aggagctcta tcgtcagccc gcctcccgct ttatggcaag ttttatgggc gacgccaacc
     7561 tgttcccggc gaactttagc gaagagtatg tcgatatcta cggttatcgc ctgccgcgcg
     7621 cggcgtattt cccggtgcaa ggtagaggca ccgtcggcgt tcgcccggaa gccatcacct
     7681 taagcaatca cggcgaagag agtcagcgtt gtgttattcg ccatgtcgct tacatggggc
     7741 cgcagtacga agtgaccgta gaatggcatg gtcaggagat tttattgcaa gtcaacgcca
     7801 ctcgtttaca gcccgatact ggtgagcact attacctcga aatccatcct tacgggatgt
     7861 ttgttttagc ggattcggca taaaaatagc gcataaaagg tcccctccag ctcagctgtc
     7921 tcttatacac aaataaccag ccggagattt gtgatgtctg tcagcctgct ttagggggga
     7981 ctctttccat ccggtcgttt ccttcctacc cgcagccatt ttttgaactt ttcttcaccc
     8041 gttgctcttc tcttatattt cattttcttg taagcgcccc atcagcgacg tcttgtgaaa
     8101 attgtcctgt ctggcaacaa tcgcgcccat ctatattgat gggctcgaac gatgaactct
     8161 caaacaacac tagatatcac cggctttcgc acttattttc cagacgcatt acgtctcaga
     8221 tttgacgagc aattatctgc ccgggcaatc gcacagcagt taagactcag ttattccccc
     8281 attcacaatc tctttcaacg aattaacgcc tcaggtattg tctgactatg acccgagtcc
     8341 ctttcattcg cccaaccgat accatacttt atgccaacag aaagaatgaa tgaaccgcgc
     8401 ctaaaatcag tgaagacaca tggcacaaag cgcgacatgt ctcacattta acccctgagg
     8461 ggggcaacaa gtataaaacc gttttttgat taagtggtcg gatatgaaaa agggaaggtt
     8521 tatgtgaaaa atacctgtgt tttctttgaa aacacacaac agtatcaggt tatttatgtt
     8581 tatacagggc gttccaaact gacctgaatt attacgcaag ttgcaacaat taaaacattt
     8641 ttgccgtagt gtgaggtact ccggtaaatt gctttccagg tgcagacaat gactaacgaa
     8701 gaactacagg caatcgcatt atccatgttt gagcaccagc gcgaaaaagt acaccagcaa
     8761 cgagagtctt tgcttaaccc atctccattc accaggccaa aacgattgga gcttgaagca
     8821 ttcttggaca aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc aagctgatag
     8881 ctcacagttt gttctcctat tcaataaagc ggataagaac catcagcgcg gtagcgtaag
     8941 gcattactgc gcattgcttt acgagtaata acaatggatt tatcaccaga agacgcaaag
     9001 tttttacgag aactgcaaca ccgtatgctc tgagaagcca ttatcgaaca gcaaacgggc
     9061 atgagcgcca tagacccatt gcgataaaaa cgggcctcat ctcctttagt tgacgacaat
     9121 tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc gcagtggtgt
     9181 aatgcagcat acgaggaaaa ccggatgaat gagaacagaa taaaaaggct ggagcaactg
     9241 ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgtt gccacatgaa
     9301 cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa aatcaggcct
     9361 gataagaaac actttttaga atgcaatgaa gacaaataac aacaatctgc atattgccac
     9421 caaccacaga tgtggcatcg ttatcagcgc gcttgatttt ctttatatcc actgacgagc
     9481 ttaccggggc acctttgttt gccggtcatt ctggtatcat gcagatattt acggcgtaca
     9541 agcccactag catgacattg taataaggcc tgagcctaac gctttaatgt gtgtcataca
     9601 ctcagtggcg taatcgttga caatatggtg catgtaatcg taagtgtcca gcgaaatcca
     9661 tctggtcata atctgaagca tccgacaaag tggtgtccac caaataagtg atctgcgccc
     9721 aatatcctga gccggaactt tgacgaaggc caacgaaaaa tgggggcgga tgttacagag
     9781 ttctcggtac aggggaaaag tggtacctgt cgccagttct ccgatctgtt taaccgggaa
     9841 attatctcct acagcttgtc agaaaggtcg gtgatggagc atcgttaata cgattctgta
     9901 cgatgcattc acagtgcccg gccagaggat gccccgtcgc tgcatacgga tcagggttgg
     9961 caatatcgaa tcgcaggcta taggcaaagt taaaagcccc atggagtagc acaaaatatg
    10021 ccgcacaaag gaaacggtct gaataacgca gtgatggaga acttcttcgg cacatagcta
    10081 aagcgcatag tgatcgagcg ctgattctgg cgaacaactg aactaacaca tcagaatctg
    10141 cattatgtta aataaatatg aaaagatggt ttaaataccc cgttactttt gacttacact
    10201 atacggtatc gcatcgttta atattcgcac cggccagatt tttacttcta ttagttgtca
    10261 caatactgaa tgcgtacgac cacagtattc tggctcctgt gtggtcatgc tttaattctg
    10321 cgttccgggc agataagcag ttgcttgcag gaatccttct tgtgttaatg tcagttcccc
    10381 tttttaccag tgctgatttc cacattccgt ccaacagagc ttatagcctt tccctggatt
    10441 atagcattgt ccggctgaag ttctttttga ataataatag aagcactgct ggcagatcca
    10501 gtccgttttt cataaccaac tgtactgata accataatct aatcagtaga aattgagtcg
    10561 aaaataagca ctactccata caggataatt agaggtcagt ttgattattc acaattcatc
    10621 atcagcattt tctgtttctg
//
LOCUS       DAZFRG010000171        10611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFRG010000171 DAZFRG010000000
VERSION     DAZFRG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.171"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..70)
                     /gene="yojO"
                     /locus_tag="ACHKUC_003496"
     CDS             complement(<1..70)
                     /gene="yojO"
                     /locus_tag="ACHKUC_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO6619694.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKR"
     gene            179..673
                     /gene="napF"
                     /locus_tag="ACHKUC_003497"
     CDS             179..673
                     /gene="napF"
                     /locus_tag="ACHKUC_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO6619695.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            663..926
                     /gene="napD"
                     /locus_tag="ACHKUC_003498"
     CDS             663..926
                     /gene="napD"
                     /locus_tag="ACHKUC_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO6619696.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            923..3409
                     /gene="napA"
                     /locus_tag="ACHKUC_003499"
     CDS             923..3409
                     /gene="napA"
                     /locus_tag="ACHKUC_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO6619697.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3416..4111
                     /gene="napG"
                     /locus_tag="ACHKUC_003500"
     CDS             3416..4111
                     /gene="napG"
                     /locus_tag="ACHKUC_003500"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO6619698.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4098..4961
                     /gene="napH"
                     /locus_tag="ACHKUC_003501"
     CDS             4098..4961
                     /gene="napH"
                     /locus_tag="ACHKUC_003501"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO6619699.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTFIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4958..5407
                     /gene="napB"
                     /locus_tag="ACHKUC_003502"
     CDS             4958..5407
                     /gene="napB"
                     /locus_tag="ACHKUC_003502"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO6619700.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5417..6019
                     /gene="napC"
                     /locus_tag="ACHKUC_003503"
     CDS             5417..6019
                     /gene="napC"
                     /locus_tag="ACHKUC_003503"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO6619701.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6032..6655
                     /gene="ccmA"
                     /locus_tag="ACHKUC_003504"
     CDS             6032..6655
                     /gene="ccmA"
                     /locus_tag="ACHKUC_003504"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO6619702.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6652..7314
                     /gene="ccmB"
                     /locus_tag="ACHKUC_003505"
     CDS             6652..7314
                     /gene="ccmB"
                     /locus_tag="ACHKUC_003505"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO6619703.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7356..8093
                     /gene="ccmC"
                     /locus_tag="ACHKUC_003506"
     CDS             7356..8093
                     /gene="ccmC"
                     /locus_tag="ACHKUC_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO6619704.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8090..8299
                     /gene="ccmD"
                     /locus_tag="ACHKUC_003507"
     CDS             8090..8299
                     /gene="ccmD"
                     /locus_tag="ACHKUC_003507"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO6619705.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8296..8775
                     /gene="ccmE"
                     /locus_tag="ACHKUC_003508"
     CDS             8296..8775
                     /gene="ccmE"
                     /locus_tag="ACHKUC_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO6619706.1"
                     /translation="MNIRRKNRLWISCAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8772..>10611
                     /gene="ccmF"
                     /locus_tag="ACHKUC_003509"
     CDS             8772..>10611
                     /gene="ccmF"
                     /locus_tag="ACHKUC_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO6619707.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWI"
BASE COUNT         2174 a         2715 c         3173 g         2549 t
ORIGIN      
        1 cccgtttttg ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg
       61 gggtatgcat ctttgacaca tcctttaata tcttagcggc tataaaaatg gcttattaat
      121 tatgcggttt atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt
      181 gaagattgat gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg
      241 tatccgtccg ccctggtccg gtgatgaatc tcattttctg acccattgca cccgctgcga
      301 cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggtg gttatccgag
      361 cgttaatttc aaaaataacg agtgcagctt ctgctacgcc tgcgcgcagg cttgccccga
      421 atcactattt tctccgcgcc acaccagggc ctgggatttg cagttcacta tcggggaagc
      481 ctgcctggcg tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc
      541 aattatcttt cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg
      601 caacggatgt ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct
      661 ccatgcacac taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt
      721 cagacatcag cacccaactg aacgccttta ccggctgtga agttgctgtc agcgacgcgc
      781 cgagcggtca gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca
      841 ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg
      901 aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc
      961 cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt
     1021 tggtcagcag gaagccatta aatgggataa agcgccgtgc cgtttctgcg gtacgggttg
     1081 cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc tgtcagggcg acccggacgc
     1141 accggttaac cgtggcctga actgcattaa gggctatttc ctgcccaaaa tcatgtacgg
     1201 taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg
     1261 cgaatttacc ccaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac
     1321 cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat
     1381 ctgggaaggt tatgccgcgt ccaagctgtt taaagcgggc ttccgttcga acaacatcga
     1441 cccgaacgcg cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat
     1501 ggatgagccg atgggctgct acgacgacat cgagcaggct gacgcgtttg tgctgtgggg
     1561 cgcaaacatg gcggagatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctttc
     1621 taaccagaac gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc
     1681 ggataacggc atcatcttta cgccgcaatc tgacctggtg atcctgaact atatcgccaa
     1741 ctatatcatt caaaacaatg cgataaatca ggacttcttc agcaaacacg ttaacctgcg
     1801 caaaggggcg acggacatcg gctacggttt acgtccgacc catccgctgg aaaaagcagc
     1861 gaagaatccg ggttctgacg cctccgaacc aatgagcttt gaagattaca aagccttcgt
     1921 tgctgaatat acgctggaaa aaaccgccga aatgaccggc gtgccgaaag accagttaga
     1981 acaactggcg cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg
     2041 cttcaaccag catacccgtg gtgtatgggc caacaacctg gtctacaacc tgcacctgct
     2101 gaccggcaaa atttcccagc cgggttgcgg tccgttctcc ctgaccgggc agccttccgc
     2161 gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt
     2221 gactaacgag aaacatcgtg atatttgcga gaagaagtgg aatatcccga gtggcaccat
     2281 tccggcgaaa atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaaact
     2341 gaatgtttac tggaccatgt gtaccaataa catgcaggcc ggaccgaaca ttaatgaaga
     2401 gcgtatgccg ggctggcgcg atccgcgcaa ctttatcatc gtttccgatc cgtatccgac
     2461 ggtcagtgcg ctggcagccg acttgatcct gccgaccgca atgtgggtag aaaaagaggg
     2521 ggcttacggt aacgccgaac gccgtactca attctggcgt cagcaggtac aggcaccggg
     2581 cgaagcgaaa tcggatctct ggcagttagt tcagttctcc cgccgcttca aaactgaaga
     2641 agtgtggccg gaagagttgc tggcgaagaa acctgaactg cgtggcaaaa cgctgtatga
     2701 ggttctgtat gccactccgg aagtgagcaa attcccggta tccgaactgg cggaagatca
     2761 gctgaatgac gaatcccgcg agttgggctt ctatctgcaa aaagggttgt tcgaagagta
     2821 cgcatggttt ggtcgcggtc acggtcacga tctcgcgccg ttcgatgatt accacaaagc
     2881 acgcggcctg cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg
     2941 taacgacccg tacgtgaaag cgggcgaagg ctacaagttc tacggtaaac cggatggcaa
     3001 agcggtgatc ttcgcgctgc cgttcgaacc ggcggcggaa gcaccggatg aagagtacga
     3061 cctgtggctc tctaccggac gcgtcctgga gcactggcat accggcagta tgactcgccg
     3121 tgtaccggaa ctgcaccgcg ccttccctga agcggtcctg tttattcacc cgctggatgc
     3181 gaaagcgcgc gatctgcgtc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt
     3241 gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc
     3301 gttcttcgac gccgcacagc tggttaacaa actgacgctg gacgcgaccg atccgctctc
     3361 gaaagagacg gacttcaaga agtgcgcggt caaactggag aaggtgtaag ccattatgtc
     3421 ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg cgcgatgtcg ttcgcacagc
     3481 aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc
     3541 tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt
     3601 gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgaccctggc
     3661 ctctggtctg tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg
     3721 tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc
     3781 gatcgacgac gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt
     3841 tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac
     3901 cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca
     3961 cagcgacgcc tgtaccggct gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc
     4021 aatcaaggtg ttaccgctgt cactggcgaa aggggagtta ggtcaccatt accgcttcgg
     4081 ctggctggag gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga
     4141 aaaaaggctg gtggcgcagt caccgttggt tagtgttacg tcgcctttgt cagttcttcg
     4201 tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg gatcttgcac ggcaactaca
     4261 gcagtagcct gttattcgac accgttccac tgaccgatcc gctgatgaca ctgcaaagtc
     4321 ttgccagcgg tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcacagtgc
     4381 tctacgccct ggcaggaaag cgattatttt gcagctgggt ctgtccgctg aacccgatta
     4441 ccgacttagc aaattggtta cgcagacgat ttgacctcaa tcagtctgcg acgatccctc
     4501 gccatattcg ctacgtgctg ttggtggtca ttctggtggg atcggcctta actggcacgt
     4561 tcatctggga atggatcaac cccgtttctc tgatggggcg tagcctggta atgggcttcg
     4621 gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg
     4681 gctggtgcgg gcacatttgt ccggttggtg cactgtatgg cgtgctgggt agcaaaggtg
     4741 tgattaccgt tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg
     4801 tttgcccgga accgcatgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg
     4861 tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat
     4921 ttacaataac tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa
     4981 agcgctgtgt caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc
     5041 taatggcgtc gattttagcc agtcgccgga agtatccggg acgcaggaag gggccattcg
     5101 gatgccaaaa gagcaggatc ggatgccgct gaactatgtg aatcagccgc caatgatccc
     5161 gcatagcgtt gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg
     5221 tgtcgaaagt tatcgcacca ctggcgcgcc gcgcattagt cctactcact ttatggacag
     5281 cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt
     5341 accgcaggcc gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg
     5401 gaaataagag gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg
     5461 gaaatggtgg cgtaccccca gccgtctggc gctagggacg ctgctgttga tcggttttgt
     5521 cggcggcatc gtcttctggg gcggctttaa caccgggatg gaaaaagcca ataccgaaga
     5581 gttctgcatt agctgccacg aaatgcgtaa cacggtgtat caggaataca tggattccgt
     5641 gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga
     5701 gtttgtgcca aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt
     5761 tggcgttatt gacacgccgc agaaatttga agctcatcgc ctgacgatgg cacagaatga
     5821 gtggcggcgc atgaaggaca acaactcgca ggagtgccgt aactgtcaca acttcgagta
     5881 tatggataca accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga
     5941 tgggcaaacc tgtattgatt gccataaagg gatagcgcac aagctgcccg acatgcgtga
     6001 agtcgagcca ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact
     6061 ttgcgagcgg gatgaacgaa ccttatttag tggtttatca tttacgctga acgcaggaga
     6121 gtgggtacaa atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac
     6181 ggggttgtct cgccctgacg caggcgaggt tctctggcaa gggcagccct tgcatcaggt
     6241 acgcgacagc taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg
     6301 gctgacggcg ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct
     6361 ggaagccctg gcgcaggccg ggcttgccgg attcgaagat attcctgtaa atcaactttc
     6421 tgccgggcaa caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg
     6481 gatactcgac gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg
     6541 tatggcgcag catacggagc agggggggat tgtgattctg actacccacc agccgctcaa
     6601 cgttgctgaa agtaaaattc gccgcatttc actgacgcaa acgggggccg catgatgttc
     6661 tggcgcattt tccgtcttga gctgcgcgta gcgtttcgcc atagcgccga aatcgccaac
     6721 ccgctgtggt tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg
     6781 caactgctgg cgcgtattgc gccgggcatt atctgggttg ctgcgctgct ttcatccttg
     6841 ctggcactcg agcgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg
     6901 ttgttgccgt tacccttgcc cgccgctgtg ctggcgaagg tgatggcgca ctggatggta
     6961 accggtctgc cgttactcat cctttcgccc ctggtagcaa tgctactggg aatggatgtt
     7021 tatggctggc aagtgatggc gttaacgcta ctgctgggaa cccctacgct tggctttctt
     7081 ggtgcgccgg gcgtggcgct gacagtggga cttaagcgcg gtggtgtgct gctcagcata
     7141 ctggtgttac cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct
     7201 tctatgcatt tgcccgttga tgggtatctg gcaattttag gcgcgttgct ggcaggcacc
     7261 gcgacattaa gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg
     7321 attactgatt tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg
     7381 cgatcccacc acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca
     7441 gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc
     7501 agcagggaaa tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg
     7561 gcatttatgc atcaatggcg gtggcagcgt ttattggcct ggtctggcag atgaaaatgg
     7621 ccaacctggc ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg
     7681 ttaccggctc tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc
     7741 tgacttctga actggtgctg ctgtttctgt atgttggtgt cattgccctg tggcacgcct
     7801 tcgacgaccg tcgtctggct ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga
     7861 atctgccgat tattcattac tccgttgagt ggtggaacac cctgcatcag ggatcaacgc
     7921 ggatgcagca aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct
     7981 tcctgctcct gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg
     8041 aaaaacgccg tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccctgc
     8101 atttgcttcc tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc
     8161 ggtggtgatg accgttattc cgctggtggt tttggtcgtg cactcggtga tgcaacatcg
     8221 cgcaattctg cgtggcgtgg cgcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca
     8281 acagcaggag gctgcatgaa tattcgccgt aaaaaccgct tgtggatttc ctgtgccgtg
     8341 ctggcggggc tggcgctgac tatcggtctg gtgctatatg cgctgcgctc gaatattgat
     8401 ctcttttata cgccggggga gattctctac ggcaagcgtg aaactcagca gatgccggaa
     8461 gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg ggagtgtgca gcgcgatccc
     8521 aattcgctaa aagtaacttt caccatttac gacgctgaag gttcagtaga tgtctcttac
     8581 gaaggcattt tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgaactg
     8641 gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga aacacgatga aaactacacg
     8701 ccgccagaag ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag
     8761 gacccagcat catgatgcca gaaattggta acgggctgct gtgtctggcg ctaggaattg
     8821 cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg cggggacgcg cgcatgatgg
     8881 cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt ggctggcgca tttctggtgc
     8941 tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata
     9001 cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggctcgctcc
     9061 tgctgtgggt gctactgatg agcggctgga cctttgcggt agcgattttt agtcagcgta
     9121 ttccgctgga tattgttgcc cgcgtactgg cgataatggg gatggtcagt gtcggctttt
     9181 tgctgttcat tctctttacc tctaacccgt tctcacgcac gctgccgaat ttcccgattg
     9241 aagggcgcga tcttaaccca ctgttgcagg atccggggct aattttccac ccgcctctgc
     9301 tctatatggg gtacgtcggt ttctcggtgg cgtttgcttt tgccattgca tctttgttga
     9361 gtgggcgtct ggacagcact tatgcgcgtt ttactcgtcc gtggacgctg gctgcgtgga
     9421 tcttcctgac gctcggcatt gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg
     9481 gcggctggtg gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga
     9541 ctgcgctgat gcactcactg gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat
     9601 tactgctggc aatcagtgcc ttctcgttgt gtctgctggg gactttcctg gtgcgttccg
     9661 gcgtgctggt atcggtacac gcgtttgcct ctgatccggc acgcggtatg tttatcctcg
     9721 cctttatggt actggtgatt ggtggttcgc tgctgctgtt tgccgcgcgt ggacacaaag
     9781 ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc tctgctgtta gcgaacaacg
     9841 ttttgctggt cgccgcgatg ctggtggtat tactggggac gctgctgccg ttagtgcaca
     9901 agcaactggg actgggcagc atttcgattg gcgaaccgtt cttcaacacc atgtttacct
     9961 ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc tctggtgcgc tgggggcgcg
    10021 atcgcccacg taaaatccgc aatttattga ttatcgcctt catctcaacg ctggtgctgt
    10081 cgctgctgct gccgtggctg ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg
    10141 caatggcctg ctggattgcg gtgctggcaa ttgcggaagc tgcgctgcgt atttcacgcg
    10201 gtacgaaaac caccttcagt tattggggaa tggtggcggc tcacctgggg ctggcagtga
    10261 caattgttgg tattgccttt agccagaact atagcgttga gcgtgatgtg cgcatgaagt
    10321 ccggcgatag tgtcgatatt catgaatatc gcttcacctt ccgtgatgtc aaagaggtga
    10381 ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt aacgcgcgat ggcaagccgg
    10441 aaacggtgct gtatgcggaa aaacgttatt acaacactgc cgggtcgatg atgaccgaag
    10501 cggcgattga cggcggcatc acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa
    10561 acggcgcgtg ggccgtgcgt ctttactaca aaccgtttgt tcgctggatt t
//
LOCUS       DAZFRG010000172        10579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFRG010000172 DAZFRG010000000
VERSION     DAZFRG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.172"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..730)
                     /gene="aroD"
                     /locus_tag="ACHKUC_003510"
     CDS             complement(<1..730)
                     /gene="aroD"
                     /locus_tag="ACHKUC_003510"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO6619708.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLR"
     gene            complement(761..1627)
                     /gene="ydiB"
                     /locus_tag="ACHKUC_003511"
     CDS             complement(761..1627)
                     /gene="ydiB"
                     /locus_tag="ACHKUC_003511"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO6619709.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1639..2904)
                     /locus_tag="ACHKUC_003512"
     CDS             complement(1639..2904)
                     /locus_tag="ACHKUC_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6619710.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(3131..4345)
                     /locus_tag="ACHKUC_003513"
     CDS             complement(3131..4345)
                     /locus_tag="ACHKUC_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6619711.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(4445..4801)
                     /locus_tag="ACHKUC_003514"
     CDS             complement(4445..4801)
                     /locus_tag="ACHKUC_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO6619712.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4937..5044)
                     /gene="rprA"
                     /locus_tag="ACHKUC_003515"
     ncRNA           complement(4937..5044)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKUC_003515"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5230..6342)
                     /gene="ydiK"
                     /locus_tag="ACHKUC_003516"
     CDS             complement(5230..6342)
                     /gene="ydiK"
                     /locus_tag="ACHKUC_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO6619713.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            6556..9612
                     /locus_tag="ACHKUC_003517"
     CDS             6556..9612
                     /locus_tag="ACHKUC_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO6619714.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTIIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTGFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9609..10019
                     /gene="menI"
                     /locus_tag="ACHKUC_003518"
     CDS             9609..10019
                     /gene="menI"
                     /locus_tag="ACHKUC_003518"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO6619715.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10119..10307
                     /locus_tag="ACHKUC_003519"
     CDS             10119..10307
                     /locus_tag="ACHKUC_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO6619716.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
BASE COUNT         2847 a         2548 c         2619 g         2565 t
ORIGIN      
        1 tacgcaaatc atttaccgag atttgccctg gcgcagaggc tttttttacc gcaccaaaag
       61 ttgccgccga cccaaatact tcaccagcca gacgagaaat tacgccagtt tttgccatcg
      121 acatcgtgat gattggacga tcggcatact gctcctgcat ctccagggtc gcggcaagca
      181 acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcgtcga
      241 aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt
      301 catggttgga catgactact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa
      361 cctgatcatc gccggtaaat aactccagat cgatcatatc aaccaggccg ctgtcgatgg
      421 ctgcacgatt gagtgcaata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg
      481 cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgctg
      541 ccgccatgac agactccaca ttggagaggt cggcatagtg gtccacacgc cattccagaa
      601 tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgctggcg atatctttcg
      661 ccatcagcga gacgatgatt ttaggtgcgc ccgtaccaat gacgagatct tttacagtta
      721 cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga
      781 cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc
      841 cttgccacaa caacatgccg tatccatcaa tcgttttgca acccgcttgt tgcgcctgct
      901 gcaataactt cgtcatatga gggttataca cgcattcagt gaccagaagt cccggatgta
      961 acagactgat atcattaacc aatgattcat tctcaagggg tttcataccc actttggtgc
     1021 cattggttaa aatgtcggcg gaagccaggg cttctgcaaa ggcttgctga tcggcgagat
     1081 cggtgactgt cacgacgcaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagagctt
     1141 tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc
     1201 cctgcgcgcc aattgccgtt gaggcaccac cggcccctaa tagcaccatc gttttgcctt
     1261 tgatatcaaa accgctctct ttaatggcgc gaatatgacc cgtgccgtcg gtgttatagc
     1321 cacgcagata gccatcatca ttaacgatgg tgttgatggc tcccaccagt ttggccgctg
     1381 gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcattgat acaccagttc
     1441 cgcgcatttt gagggctttt aatccttcaa ttgctccggg aaaactatcg ttatccactt
     1501 caaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcgg
     1561 gcgataaact gtggcggatg ggataggcca tcaacccaat caattcgtat tttgcggtaa
     1621 catccataat taactccttt aacctctatg cttaattgtg tttaaccggg tgctaaattt
     1681 atgctcgcca aaccgcacat cattttcagg aataatgaaa acgcggtaat agcggataaa
     1741 aacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata
     1801 ttgcaggccg atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc
     1861 acccatcatc atatatatac tggtgacttt ggctttgctt ttggggaaaa actctgacat
     1921 gaccgaaacg ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggctcc
     1981 ggcattacac accaatggag aagggtacag gtagataatg gcagctgtta ttgttgccag
     2041 tgcagagtta aatacattag cccagatggg acggaccatt tttttcagta gtgcggcaaa
     2101 aataaagaca cagaccagcg aacccatact gtaataagag atggttttta atgcctcagc
     2161 ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa
     2221 ggtcgagaat gcggctacac cgaacagtac tgatgaaaca ccttccagcc agactaacgg
     2281 tttgctgttc atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa
     2341 tttgcttttc aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc
     2401 atagccgtac cagatgttat tgagcaacat atagctcacc agcattgggt agaacatttg
     2461 tccgaatgac accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca
     2521 ttccataagc gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac
     2581 tgccagacag taggcgagag ttaaattcgg gcaagcagga ataccaaaga agaatagcag
     2641 atacattatt actgccatta atatcaccgc ccgacgacca aacttatcgg agatcactcc
     2701 gaagaataaa atactgatca atcgccccaa tccgataccg gaaattaaat aggcaatgcc
     2761 cgcgttgtca gtggaaaact tttctgccaa agatgtcatg ttttgggcaa gcgtaataac
     2821 actaatgccg tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt
     2881 gctgaaagcc ttattttgag acattttcac agctccttgc tgaaagataa tttcgttacc
     2941 gtacgtattg ttctccgacg gtttacataa ttactatact gaagataatt ttccctgggt
     3001 acatggcata aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg
     3061 attttcctta gttagcgcag aaagagttat ttatccgtaa gatcttgcat aacctgaaaa
     3121 gaatattgca ttacccaccc ggagcgatat tttcctttag cgtgtgatgc cgcgtttctt
     3181 tgcgactgcg taagccaata aacagtgcca gtataaagcc gatagccgcg atggcggtat
     3241 cgaaccacat aatatcggca atacttcttt gcgaaagatg agccgtaatc aacggaatgg
     3301 taaatgttgc aatgctgccc gcactgtaat agatccccgt tgcttttcct ttagcgtagg
     3361 gaaaacgttc cgccattaac gtcaggccaa tttgtacaac accgcctgcg gaggtaaaac
     3421 cgatgacgaa agcaaatatt atcaccacat aaaatgtggg atgcaggcag acggtgagca
     3481 gggcgataaa tgagataaag gtgtacagca tcagtaatgt tgttgggcga acggtattac
     3541 gaatgagtgg agcagtaata aatacacaca gtagcgaacc cacggtgtag atactgagta
     3601 atttgatcga catggtgtat gacatgccag cgacaaattg tccgtattgt gccagccact
     3661 ggctaaccag ataaaacgtt gccattgaga tatagccata tagggaataa ctggctaaat
     3721 cgataattga acagcgatgt tccgtagagc tggtggtttt ctttatgaca ggtaagtgac
     3781 gacccggatg gggtgggaac gtacaacggt ataaaaacag agcgttaata aacataatgc
     3841 ctgcggcaat cataaaggac caaccgaacc acagttcagc ccacactaac aggctgataa
     3901 ttagcggtaa taaaaactgt ccgctggaaa caaatgcttt aattaaaata ttggctgtac
     3961 ccggtgagcg tggaaaagct tccatcaaac tgggataggt gcctgcatcg agaaaactgt
     4021 ttgccattcc cgccagaaag ccaaaaacat aagcgataat gatgttattg gtgtgcagga
     4081 tgccaaaaaa gaaggccata tagcagcaca tcccgagcat gataaaaggg cggcgaccaa
     4141 agcgatcgga taataatcct gcaaaaagca agacacttaa tcgaccaatg cccagtgatg
     4201 agataactat cgagacgccc gcggcattag tctgccaaag cgtctccagc gaggccatat
     4261 tcaggctcat caaaatgacg cccataccat gcaccaggta attaaaatac aacccaagtg
     4321 cggtagggta atagggattt ttcataggtt ctacttagca aaggtgcttt aaaaagtgta
     4381 gcgatgttgt gtcagtatcc gttcattatt gttgtaacaa cgacaattcc tttctgtgga
     4441 gaaattaaca aagccgctcc agatcgtgtg catataattc agcggctacc gattgataga
     4501 tcttccagtc aataaaattg ccatcagggt agacttgttg aaacgcggtt aagtcgggga
     4561 aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt ctctatgatg gcatgaagat
     4621 gtttttttcg ttgctgacgc atagccaaca gttgctcgac tacacaatca ggaatggcgc
     4681 atttgccatt ttcccactga cgccagcttt cactgttacc ggtctgggca atccaggtcg
     4741 cgcattcatc aatagtcatg gcaaaaatat ggcgaagtgc ctggagttca taagcgttca
     4801 tcgtgtaatg gctgcatgaa ataaacaata aaatataaat aacattatca gcctgctgac
     4861 ggcttgaaga gagtcacagt atcttgtgca acattattgg aaagagtgag gggcgaggta
     4921 gcgaagcaga aaaatgttaa aaaaaagccc atcgtgggag atgggcaaag actacacaca
     4981 gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt tgatttataa
     5041 ccgtgagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc tcattagttt
     5101 aaaaactgta aagaatttgc gacaggtact accagctgaa tagcggtaag caggaaaata
     5161 tggggaagag caagcaaata tcaattagca ggggttaaca atcaggcggc agagccgcct
     5221 gatgagaaat tacttattcg atttctcgat ttcgccaagt tcttcgagaa tttcttccgg
     5281 ttggtccgtc ggcggtggga cttcttccac ccacgcggca aacagacgcc aggaaacggc
     5341 taacagaacc ggaccaataa acagaccgat catcccgaaa gcaatcaaac caccaataac
     5401 gccagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca ttgggcggat
     5461 gacgttatcg agcgtgccaa ccacaccgct ccaaactaac aataccgttc cccaggtggt
     5521 atcgccagtc cagtagagcc agataatcgc cggaatcagt accggcaacg gtccaagctg
     5581 gacaaggcag gagaggatca ttaacaccgt tagcaaagtt gcataaggta cgccggatac
     5641 ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca cacccagcgc
     5701 caccgcgcgg atagcctgtg ccgccagcag cacggcggca tctccgcgaa cgcctgccag
     5761 acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt acagcagggc
     5821 gctgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc
     5881 aacgaaccag gtggtggtgg tgccaatata aggacggact ttcgccatga tcgccgtccc
     5941 ccccatatcc agcaaattgt gccagcctgc atatagcttc gcgccaatca ccggaatggt
     6001 attaagccac gccagatcgg gtaacgtcat gtcaccactg gaaatggctt taattagcgg
     6061 gccgctgccg tcgacgatac tgttaaccag caaagcaata gggatgataa acaccatcac
     6121 taataacagc gtcatcacca gaacggcgag agagcggcga ccaaacatga tcttttgcaa
     6181 acgtaacaat accggccagg tggcgataac cactgtaccg gcccatgcaa agccgagaat
     6241 aaagggttgt acaatccaca gacatgccac aatcatgatg gctaaaaaca gcaccgaaag
     6301 cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt ttcacctttt
     6361 ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc
     6421 aatttcagcg gtttttaaca ggtcgattct gcccttaatt ctgatgggcg tgttagaaaa
     6481 aaatgtggta caacaactca ggcaacacgc aaacgattaa tctctcatca cagaccgcag
     6541 gaaagggtca atataatgat tccacagatt tcccaggcac ccggcgtcgt tcaactggtg
     6601 cttaattttt tgcaagagct ggagcaacag ggttttaccg gcgatacggc gacaagttat
     6661 gccgatcgtc tgacaatgtc gactgacaac agtatttacc aacttctccc cgatgcggtg
     6721 gtatttccgc gttcaaccgc agatgtagcg ctgatcgccc gtcttgccgc gcaggaacgc
     6781 tattcatcgt tgatctttac cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc
     6841 aaccagggga ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct
     6901 gaagagggct gggtgcgcgt tgaggccggg gtgattaaag accaactcaa tcagtacctg
     6961 aaaccctttg gctacttttt tgcgccagaa ctttcgacca gcaaccgggc aacgctcggc
     7021 gggatgatca ataccgatgc atccggtcag ggatcgctgg tctatggcaa aacgtcagat
     7081 cacgtacttg gcgtacgcgc ggtgttgttg gggggcgata ttctcgatac gcaaccttta
     7141 cccgtcgaac tggcggaaac gctgggtaaa tccaatacca taatcgggcg aatttataac
     7201 acggtttatc aacgttgccg tcagcaacgc cagttaatca tcgacaactt ccccaagctt
     7261 aaccgctttc ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgggttc
     7321 gacctgacgc gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcga
     7381 ctggatatta cgcgcttgcc taaagtacgc cgtctggtga acgtcaaata tgactctttt
     7441 gactccgcgc tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg
     7501 gtggactcaa aagtgctcaa ccttgcgcgg gaagatattg tctggcattc cgtcagcgag
     7561 ttgattaccg atgtgcctga taaagagatg ctcgggctga acattgtgga atttgccggt
     7621 gatgatgagg cactgattga cgagcgagtt aatgcactct gtgtgcggct tgatgagctg
     7681 attgccagcc aacaggcagg agtgatcggc tggcaggtgt gccgcgagct ggcgggtgtt
     7741 gaacgtatct atgcgatgcg caaaaaagcc gttggtctgc ttggcaatgc caaaggtgct
     7801 gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc cggaacacct ggcggattat
     7861 attgctgaat ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac
     7921 gtcgacgcag gtgtcttgca cgtccgtcca gcactggata tgtgcgatcc gcaacaggag
     7981 attttgatga agcaaatctc tgatgatgta gtggcgctta ccgcgaaata cggtggtttg
     8041 ttgtggggcg aacacggcaa aggttttcgc gccgaataca gcccggcgtt ttttggtgag
     8101 gaactttttg cagaactgcg taaagtgaaa gcggcgtttg atccgcataa ccgactcaac
     8161 cccgggaaga tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg
     8221 aagcgcggta cattcgatcg acagatcccc attgcggtac gccagcagtg gcgtggtgca
     8281 atggagtgta acggcaacgg tttatgcttc aactttgatg ctcgtagtcc gatgtgtccg
     8341 tcgatgaaga tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac gctggtacgt
     8401 gaatggctgc gtttgttggc ggatcgcggc gttgacccac tcaaactgga acaagaactg
     8461 cctgaatccg gcgtcagttt gcggacgtta attgcccgca cgcgcaatag ctggcatgcg
     8521 aataaaggcg aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc
     8581 tgtaaagctt gttcgaccca gtgccccatc aaaattgatg tgccggagtt tcgctctcgt
     8641 tttctgcagc tctatcacac ccgttattta cgccctctgc gcgaccacct tgtcgctacg
     8701 gtcgagagct acgcaccgct gatggcacgc gcgccgaaaa cctttaactt cttcattaac
     8761 cagccgctgg tgcgcaaact ctcggaaaaa catattggca tggttgattt gccgctgctg
     8821 tcggtcccct cgctgcaaca acaaatggtg gggcatcgct cggcaaacat gacgctggaa
     8881 cagcttgaag ctctcaatgc agagcagaaa gcgcgcacgg tgctggtggt gcaggacccc
     8941 tttaccagct attacgatgc tcaagtggtg gcggattttg tccgtctggt cgaaaaatta
     9001 ggtttccagc ccgtgttact gccattttcg ccaaatggaa aagcccagca tatcaaaggt
     9061 tttcttaatc gttttgcgaa gacggcgaaa aagacggcgg atttcctcaa ccgtatggcg
     9121 aagctgggta tgccaatggt aggcgtcgac ccggcgctgg tactttgtta tcgcgatgaa
     9181 tataaactgg ctctgggcga ggagcgtggc gagtttaacg tcttactggc aaatgaatgg
     9241 ctggcaagcg cacttgagtc acagccagtg gctacagtca gcggtgaatc atggtatttc
     9301 tttggtcact gtaccgaagt taccgccttg ccgggcgcgc cagcacaatg ggccgcgata
     9361 tttgcccgtt ttggcgcgaa actggaaaat gtcagcgtgg ggtgctgcgg catggcaggg
     9421 acttacggac atgaagcgaa aaaccataaa aattcgctcg ggatctatga attatcctgg
     9481 catcaggcta tgcagcgact gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt
     9541 agtcaggtaa aacgggttga aggcacaggg gtacgccatc ctgtgcaggc tttactggag
     9601 attattaaat gatatggaaa cgaaaaatca ccctggaagc actgaatgct atgggggaag
     9661 gaaacatggt gggattgctg gatattcgct ttgaacatat tggtgatgac acccttgaag
     9721 cgacaatgcc agtagactca cggacaaagc agcctttcgg gttgctgcat ggaggtgcat
     9781 ccgtggtact ggccgaaagt atcggttccg ttgccggtta tttatgtacc gaaggtgagc
     9841 aaaaagtggt tggtctggaa atcaatgcta accacgtccg ctcggcacga gaagggcggg
     9901 tgcgcggcgt atgcaaaccg ttgcatctcg gttcgcgtca ccaggtctgg cagattgaaa
     9961 tcttcgatga gaaagggcgt ttgtgctgtt cgtcacgatt gacgaccgct attttgtgac
    10021 gttgtcacca gaaaagtgtg acgcatattg cttttggtta ttatctgcac agtaaacggt
    10081 ggtatactgt gcaggtcttg tctaaaagcg aggatgctat gtctactcaa cttgatccca
    10141 cccaactggc cattgaattt ttacgccgtg accaaagcaa tctttcacct gcccaatatc
    10201 tcaagcgtct gaaacaatta gaacttgaat ttgctgacct gcttactctc tcttctgctg
    10261 aattaaaaga agaaatctac ttcgcctggc gtctgggcgt gcattgatcc tgatttaatg
    10321 tcgcgagttc gccaaatcgg cctggattaa tcttcgtcca ggttcgaggg cctttttaat
    10381 acagcctatc tacccgcctg ataacgttca aaaataatac aaatcgcagt ttgtgttaaa
    10441 acagtgggtt agctttatga gattatcctt atcgcccctt caagagctaa gccactgtga
    10501 gtgccggaga taagcgccgg atggggtaga aacccttaag cctgtgtcgc acagacttaa
    10561 gggttttctt atttctgaa
//
LOCUS       DAZFRG010000173        10476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFRG010000173 DAZFRG010000000
VERSION     DAZFRG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.173"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..772
                     /gene="artJ"
                     /locus_tag="ACHKUC_003520"
     CDS             41..772
                     /gene="artJ"
                     /locus_tag="ACHKUC_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO6619717.1"
                     /translation="MKKLVLAALLTSFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(801..1928)
                     /gene="rlmC"
                     /locus_tag="ACHKUC_003521"
     CDS             complement(801..1928)
                     /gene="rlmC"
                     /locus_tag="ACHKUC_003521"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO6619718.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTVDLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLTQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLILLVKQ"
     gene            complement(1969..2457)
                     /locus_tag="ACHKUC_003522"
     CDS             complement(1969..2457)
                     /locus_tag="ACHKUC_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO6619719.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2517..3362)
                     /gene="potI"
                     /locus_tag="ACHKUC_003523"
     CDS             complement(2517..3362)
                     /gene="potI"
                     /locus_tag="ACHKUC_003523"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO6619720.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3359..4312)
                     /gene="potH"
                     /locus_tag="ACHKUC_003524"
     CDS             complement(3359..4312)
                     /gene="potH"
                     /locus_tag="ACHKUC_003524"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO6619721.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            complement(4322..5455)
                     /gene="potG"
                     /locus_tag="ACHKUC_003525"
     CDS             complement(4322..5455)
                     /gene="potG"
                     /locus_tag="ACHKUC_003525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO6619722.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5550..6662)
                     /gene="potF"
                     /locus_tag="ACHKUC_003526"
     CDS             complement(5550..6662)
                     /gene="potF"
                     /locus_tag="ACHKUC_003526"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO6619723.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMVF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7012..7488)
                     /locus_tag="ACHKUC_003527"
     CDS             complement(7012..7488)
                     /locus_tag="ACHKUC_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO6619724.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7576..8478)
                     /gene="rimK"
                     /locus_tag="ACHKUC_003528"
     CDS             complement(7576..8478)
                     /gene="rimK"
                     /locus_tag="ACHKUC_003528"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO6619725.1"
                     /translation="MKIAILSRDGTLYSCKRLREVAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8539..9261)
                     /gene="nfsA"
                     /locus_tag="ACHKUC_003529"
     CDS             complement(8539..9261)
                     /gene="nfsA"
                     /locus_tag="ACHKUC_003529"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO6619726.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9245..9532)
                     /locus_tag="ACHKUC_003530"
     CDS             complement(9245..9532)
                     /locus_tag="ACHKUC_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO6619727.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMIPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9692..9949
                     /gene="grxA"
                     /locus_tag="ACHKUC_003531"
     CDS             9692..9949
                     /gene="grxA"
                     /locus_tag="ACHKUC_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO6619728.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(9979..10356)
                     /locus_tag="ACHKUC_003532"
     CDS             complement(9979..10356)
                     /locus_tag="ACHKUC_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO6619729.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         2770 a         2857 c         2502 g         2347 t
ORIGIN      
        1 acggccaaaa atcataagat tgacagacgg gagttccatc atgaaaaagt tagttcttgc
       61 cgcattactt acttccttta ctttcggtgc ttctgccgca gagaaaatca attttggcgt
      121 ttcagccacc tatccaccct ttgaatctat aggtgctaat aatgagattg tcggctttga
      181 tatcgacctg gcaaaagcct tgtgcaaaca aatgcaggca gaatgtactt ttactaatca
      241 cgcgttcgac agcctgatcc cgtcactgaa attcagaaaa tatgacgccg taatctccgg
      301 aatggatatc acgccggagc gtagcaaaca ggtatcgttt accacgccct actacgaaaa
      361 ctcagccgtc gtgattgcca aaaaagatac ctacaaaacg tttgccgatc tgaaaggcaa
      421 acgtattggg atggaaaacg gcaccacgca ccagaaatat attcaggatc agcacccgga
      481 agtgaaaact gtctcctatg acagttatca gaatgccttt atcgatctga aaaatggccg
      541 tattgatggc gtatttggtg acaccgcagt ggtaaacgaa tggctgaaaa ccaatccgca
      601 actgggcgtt gctactgaaa aagtgaccga tccgcaatac ttcggcaccg gcctgggtat
      661 cgctgtacgt ccggataaca aagctctgct ggaaaaactg aataacgcgc tggcagcaat
      721 taaagctgac ggtacatatc agaaaatcaa tgaccagtgg ttcccacagt aagattcaga
      781 aagccgcacc tgcggctttt ttattgcttc accagcagta tcagcacttc gtaatgcgca
      841 gtgtgcggga acatatcaaa aagctgtacc cgttcgatgc gaaacccagg cagttcgcgg
      901 atatctttcg ccatggtttg ggcgttacag ctggagtaga taataaaacg cggtgccatc
      961 gttgagagat aatcacacag cggtttacca atgccgcggc gcggcgggtt aaccagcacc
     1021 agctccggca cttccccctg ggcggtggca aactgagtgg agtccagcgc ctgaaattgc
     1081 aaacgcgtta agcccagttc agcggctgac tgcttcgcac aggcaatggc ctctggtgca
     1141 atttcgatcc cggttaactg catgtcaggc gtcgcgcagt gtaaaccaaa gccacccaca
     1201 ccgcagaaca gatcccacat atgtttaacc ggcagctgtc gtacccagtc gcgcgcggtg
     1261 gcgtacagct ggctggcgac cgccggatta gtctggaaga aactttgcgg acggatccac
     1321 agcggtacgt cattaaaacg ctctgccagt gcctgttgtt cggtcaggta gatctccgtc
     1381 tccccttcca taatcgccat atgtaccggc tgaatattga cggtaataac tttcagctgc
     1441 ggtaattgtt cctgtaacca cggcagcgcc ttacgcagtt gcgtcagttt ggtatcagaa
     1501 cgcagtacaa aacgcagcat catgccgcca tcgctctggc tttcagtcag cagaatgtat
     1561 ttcagttcac cacgtttacg cgccacgttg tagggcgtta accccgcacg ggcgataaac
     1621 ggttttagcg ccgcaaaaac gggcgcaaat gaggctggat aaagcgggca gtcacaaagg
     1681 tcttccggtg taccatctcg atgcagcata ccgagcagtg gtttttcaac gctaccactc
     1741 accaccattt tggctttatt acgaaaccct tgttccgggc ctgacaccgg cgcacaccat
     1801 tcctcaaccg gaaaatcggc gagcagattt ttaagatcga cggttttagc ggagagttgc
     1861 tctggaatcg gctgcgttat ccactgacag gaacgacagc gacccgcgtc gtaaagtgcg
     1921 cactgcatac attgaccttc acatcatcag gggcgacgat tatacacatt attgcaactg
     1981 gaagaaccgc cgactagtcg agggaacgaa cagcagcatc aggatgagca tatccggcag
     2041 cttctgcagc atcaggctat ggaagatttc acgttttgat tcaccgggaa tgctgaacag
     2101 ctccggataa ccatacccca gcgaggccgc ccacaagtaa cttgcggctg tgatttgtgt
     2161 caacagatac agccagcgcg cccagcgacg ccctttcacc agtgaaaacg cacaccagat
     2221 ctcaatgaaa accagcacca gactgctcaa aaagaccagc gttaaactcc aggtttgtac
     2281 gctgcgatga atgaactcgc caataccgcg cacgcccagc gtattaaaaa tcatcagcac
     2341 gtcgaggcca cggatcataa taatggcgag cgccgccacc tgcaccagcg caggcacatt
     2401 caggcgagca tgagatgaag atgttttctt aaaaaatccc aacgtttcgt cttccatgaa
     2461 aacaatgccg cgacatgcgc ggcattatgt agccaggttg gcaaatttta gtgtcttcag
     2521 ccacgtcttg cacgctggat gtcgcgtatc cgctgttttt ctgcgcgcgc catcagatac
     2581 caggcgataa atccgacaat tccgaccgcg ccgaggatca atgttgccag ggcgttgatt
     2641 tccggattca cccccatccg cacgctggaa aagaccagca tcggtaaagt ggtggctccc
     2701 ggcccggaaa caaagctggc gatcaccaga tcatcaagcg acaaagtaaa agccagtaac
     2761 cagccagaaa tgatcgcggg catgatcatc ggtagcgtaa tgacaaaaaa cactttcagc
     2821 ggcgtcgcac cgagatccat cgcggcttct tctatcgagc gatccagttc ccgcagacgc
     2881 gacgaaataa cgactgccac ataagccgta cagaaagtga catgcgccag ccagatggtg
     2941 agcataccgc ggtccgcagg ccagccaata gcatgagcaa gcgcgacgaa taacaacaac
     3001 agcgacaaac ccgtgatgac atctggcatt accagcggtg cggtgatcat gaaggcaaaa
     3061 ccatttgatc cgcgaaacct gccaaagcgc accagcacga ccgccgcaat agtcccgagg
     3121 atcgccgccg ccgtcgccgc acaggccgca attgtcagac ttaaaccaac cgcactcatc
     3181 attgcgtcat cgcgcaataa ttcgccatac cagcgcgttg accagccggc ccacaccgtc
     3241 actaacttcg agctgttaaa cgaatagata acaagcatca gcattggcgc gtagagaaag
     3301 gtgaagccca acagcaaaat cacaatccgc cagggcgaac gaaccaccgg taaattattc
     3361 atccgtgttc tcccacgctt ttttgctgat gtttgtggaa ccacattatc ggcataatta
     3421 gcagcagcaa catgatgatc gctaccgctg aggccaccgg ccagtcgcgg ttgttaaaga
     3481 actcctgcca cagcacgcgc ccgatcatga tgctgtccgg gccaccgagc agttccggga
     3541 tcacaaactc gcccacagcc gggataaaca ccagcatcga tccggcaata atcccacctt
     3601 tagtcagcgg cacgatcaca gtaaagaacg ttttcagcgg tcgtgcaccg agatccagtg
     3661 ccgcttctac cagcgaataa tcaatacgaa tcaacgcggt gtaaatcggc agcaccataa
     3721 acggcacgta ggcgtaaaca atgccaatat aaaccgcaaa gttggtatgc agaatggtca
     3781 gcggttgatc gataaccccc agccacagca gaaaattatt cagcacaccg ttgtttttta
     3841 atattcccat ccaggcgtag acacgaatca gaaacgaggt ccacgacggc aggatcacca
     3901 gtagtaataa gatattacgg gtcgaaggct tactgtgcgc caccgcccac gccagcggat
     3961 agccgatcag taaacagcaa atagtcgaaa tcgccgccac ctgtaacgac tggagataag
     4021 catcaaaata gagcggatcg tcagtcagtt gcagaaaatt accgagatta agagtgatag
     4081 aaagttgccc gtcagcccac tccatcaatt cggtataagg tggaatagcg cgcgccatct
     4141 ccgccaggct tattttaaag acgatcagaa atggcagcag aaacagcaag atcaaccaga
     4201 tatatggcaa cgcaatgacc agtttgcgcc catgcttcat ttgcagctgc gacaaccaca
     4261 gcttaaatcc gcccggcggt ttcgactggg cagcaggttc aagtgtactc atctttgctc
     4321 cttaaaccgt cagcaccaca cagctgtcca cttcccagca caaacgtact tcgtcgcccc
     4381 aggtcggtaa ccctttacga tggcgatggg cgttttgtag ctgggcgcta atcatctgcc
     4441 cacttttcag gcgaacgtga tacaccgaaa gatcgccgag ataggcaatg tgtatcacct
     4501 cccccaccgc gaagttacaa ccattggcgg gcggttcctc gcaaagcatg attttttccg
     4561 ggcgaagcgc cacatgcacc ggcacgttat caaccactga agcatccgca tcgactttca
     4621 gtggatgcac cagccccggc gaataaagca ccagaccatc ttcctgacgc tctttgagta
     4681 caccttcaaa gacattaacc gaaccgataa attcggcgct gtagcgggtg gtcggatgct
     4741 cgtagatctc ttccggctcg ccaatctgga caaatttccc acgattcata atggcgatgc
     4801 gccccgccat agtcatcgcc tcttcctgat cgtgagtaac cattacgcag gtcacaccga
     4861 cgcgctccag aatatccacc acttcaagct gcatccggtc acgcagcttt ttatccagcg
     4921 cgcccatcgg ctcatcgagc agtaatagtt tcgggcgctt ggcaaggctt cgggccaggg
     4981 ccacacgctg acgctgacca ccagaaagct gatgcggttt gcgtttggcg aactcctgca
     5041 tatgcaccag cccaagcatc tcattgaccc ggctggcaat ttccgctttc ggtagtttgt
     5101 cctgtttcag gccaaaagcg atgttctgtt ccacggtcat atgcggaaac agcgcgtaag
     5161 actgaaacat catattgatg gggcgcaggt aaggcggcac ctgtgacaaa tcgacgccat
     5221 cgagcataat ctgcccggca gaaggttgtt caaaacctgc cagcatacgc agcagcgtgg
     5281 acttgccaca gccggatgcg cccagcagcg cgaagatttc acctttgtag atggtcaggc
     5341 tgacatcatc caccgcatgt tgaccatcgt aggatttggt caggttacgg atttctaata
     5401 gcggcgtcag cgccttacgg gttttcgcct gcgggcgagg gatagcgtca ttcacggctg
     5461 ctctccggca ttgaatgtgt gcaagcgcac catgatggtg cgaattgccg gcgggtgtgg
     5521 tgcgcccgtc cggcatctac ggctgcggat tattttccgc tcttcacttt ggtccacgcg
     5581 cgggtgcgca cacggtcgat tttcgggtcc tgcactttca gagtgaacaa cttcgcacga
     5641 acatccgcag gcggataaat acccgggtta tcacggactt ccgcactcac cagcggcgtg
     5701 gctgctttat tggcgttagc atagaacaca tggtcagaga tatgtgccac cacatcagga
     5761 cgcagcaggt aattcaggaa ctgataggct tcgtctttgt ttttggcatc cgcaggcatg
     5821 gcgaatacat caaagaacac catcgcccct tcttttggaa tcgagaacga gacattcacg
     5881 ccattcttcg cttccttcgc gcggtttgac gcctgccaga catcgcccgc ccagccaata
     5941 gcgacacaga tgtcaccatt cgccagatca ttgatgtatt gggatgaatg gaaataacga
     6001 atgttcgggc gcagctttaa cagcagatcg gtagccggtc cggtgtaatc atccgcttta
     6061 gtgctgttag gatctttgcc gagataattc agcacggtag caaacacttc ttctggcgca
     6121 tccaggaaag agacaccgca gcttttcagt ttttccagat tttcaggttt gaggatcaag
     6181 tcccagctat cgacgggcgc gttttcgccc aacaccgctt taactttatc gacgttatag
     6241 ccaatcccgg tcgtcgccca catatagggc atagcaaatt tattgtcggg atcgtgtttg
     6301 gcgaccaact tcagcagctc cggatcgaga ttcttccact ccggcaattt gcttttatcc
     6361 agcggctgga aaactcccgc tgtcagctgg cgctcaagaa agcttgctga cggcaccacc
     6421 agatcaaagc cagtgctccc cgccattaat ttgccttcca gtacttcgtt agaatcgaaa
     6481 acatcgtaga cgactttaat accggtttct ttttcaaagt tggccaccgt gtccggggcg
     6541 atataatcag accagttata aatgtggagt gttttttgtt cagccgcgag cgtgctgaca
     6601 gagacggcca tcagagcccc cgcaaccaga cccgatagcc attttttatt taaggcggtc
     6661 atatctcttt ccttctgaaa attcgttaac aaacgaacaa aagttgtcac actgaaagat
     6721 atgcaataaa cgtgcatatt tttatcggcg cggtgtaaga aaagagaact ctctcccggc
     6781 ctgcactgct cactatataa aagcatcgca agaataactt tagccagggt ttgagactat
     6841 agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct atgtgataaa
     6901 gccaaatcgg cacaagagaa gtgaattaat ctttaataaa aggttaaaaa cagcggataa
     6961 ttttgcggca cgcagtcact gcggcagcga ctgcgtgctc gaaaggagtt atcagtggag
     7021 gaaatagttt tcggtaacgt tattttgccc ttcatcgtca gtcggtaaca gcagttgatg
     7081 ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc
     7141 aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca ccagctttgg
     7201 cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg cattgatggc
     7261 tgataaatcc gccgccagcg gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa
     7321 caggatcgtg ttatcgatca gatcgatttt agcatcaaag acaccgtcga aattctgcat
     7381 atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc ccaggtcatc
     7441 gagccattga cgcaacgtat ccagaccagg aacgaccagc gatgtcataa ttttgtacaa
     7501 cctcttccga taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa accatgatca
     7561 gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag cgtggcgttc
     7621 gatccagcgg atcattttac ccgcgatgtc gataccggtg gttttttcta ttccttccag
     7681 ccctggcgac gcattcactt ccatcaccaa cggcccgcga ttagcacgca gaatatccac
     7741 accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg
     7801 tgtgatactg gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa aatcgccctc
     7861 tttcgctcgc cgttcaatcg cagcgactac ttcatcgcca acaaccagac agcggatatc
     7921 gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca gaccgcggaa
     7981 agcgtcaatc acgctttccg ccgcctgacg cgtctccgcc agcacgacac caattccctg
     8041 cgtgccttca accagcttga ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct
     8101 ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct gacgcgccag
     8161 cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg
     8221 atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg taatggcggt
     8281 gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt agtgaataga
     8341 agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt caaccaggtg
     8401 accgcgctgt atagcgactt cacgcagccg cttacacgaa tagagcgttc catcccggga
     8461 caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca
     8521 tacatcgacg tggcagtttt agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat
     8581 aaatgggcgg ctttctttaa tgattgttcg gcggatatga tcgctccagg tatcccggcg
     8641 attattgctg ccacgggtga ggtaatattc cgccagttgc tcgtcatact gcgccagtgc
     8701 gtctttatcc agcggttgat agctgttttc atgcaccaaa atggaggccg gtaagcgcgg
     8761 cttaagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg gcagaacatg
     8821 ctgcggtaat ttcagcagtt ccgtcaccgc ttcaatatta ttgcgcaggc cgccgatata
     8881 taccccgccc aatcccagcg attccgctgc ggttaatgca ttctgcgcca tcattgccgt
     8941 atcaacgaca ccgagcaaca gttgttccgc caggccgagc tgagcatccg gacagatctg
     9001 taaatggcgg ttaaagtcgg cacagaacac ccaaaactcc gccgcttgtg ctacgtgttt
     9061 ttgcccgccg gtcagcgtca ccagttcttc acgtaacgct ttgtcggtaa tgcgaataat
     9121 gctactgcac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc
     9181 acgctgcgct tcggaaatgg gttcatcagt gaaatggcga atggagcgat ggccacaaat
     9241 aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatat tgcggtggat
     9301 ggttcatcag ctgtggggca agacgttttg ccacctgaag aatcaccacc accgcagcgg
     9361 gaatcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc cgagaaaatg
     9421 gctcaggtag cgacagggag tcgcttaccg acagcagcgc caccgccagc aacatcattc
     9481 cgataaattc cagtatcaac acgcttttag gcaatttacc gatcgcgcgc atacgcttcc
     9541 ctctgcaaag tgagccttca gtctaaaact tttcactgca ttgtgtttaa cagttatagc
     9601 ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt agcgtgatca
     9661 ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg gtcgtccggg
     9721 ttgcccttac tgtgtgcgtg caaaagatct ggccgagaaa ttgagcaatg agcgcgatga
     9781 tttccagtat cagtatgtag atattcgtgc ggaagggatc actaaagaag atctacaaca
     9841 aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg
     9901 cggctatacc gattttgctg catgggtgaa agagaatctg gacgcctgat cgtctgacaa
     9961 gccctcgcgt tgagggcttt attgattttt tctgtgctgt ggtttaaaca agctactgat
    10021 aaataagaaa cacaatgccc ccagcgcaca ccagaacacc gcacttaata accatgccag
    10081 ctcttgccag aatgagcgcg tcggtgaaaa aaacagccgc ataatgagca tcgaacaggg
    10141 tgccgccagc attgcgccaa acagaggttt caggacttct ctacgatgtg aaaagaagct
    10201 ggcaactgca ccaggaagaa tgaaaaatag caaaccgatt tcaggatgcc cggcagcccg
    10261 aaaagcgcct ttcatgtgcg tcgccagaaa aagacacacc acaatgaaga ggacaaaaca
    10321 gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac actgcgtcta
    10381 tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg cattccatgc
    10441 taatataggc caacgcaatt catatagccg ttgata
//
LOCUS       DAZFRG010000174        10347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFRG010000174 DAZFRG010000000
VERSION     DAZFRG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.174"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..105)
                     /locus_tag="ACHKUC_003533"
     tRNA            complement(30..105)
                     /locus_tag="ACHKUC_003533"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Thr,seq:cgt)
     gene            complement(220..1473)
                     /gene="proA"
                     /locus_tag="ACHKUC_003534"
     CDS             complement(220..1473)
                     /gene="proA"
                     /locus_tag="ACHKUC_003534"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6619730.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVIIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(1485..2588)
                     /gene="proB"
                     /locus_tag="ACHKUC_003535"
     CDS             complement(1485..2588)
                     /gene="proB"
                     /locus_tag="ACHKUC_003535"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6619731.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            2877..3932
                     /gene="phoE"
                     /locus_tag="ACHKUC_003536"
     CDS             2877..3932
                     /gene="phoE"
                     /locus_tag="ACHKUC_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6619732.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(3971..4372)
                     /gene="crl"
                     /locus_tag="ACHKUC_003537"
     CDS             complement(3971..4372)
                     /gene="crl"
                     /locus_tag="ACHKUC_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6619733.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTV"
     gene            complement(4430..5674)
                     /gene="frsA"
                     /locus_tag="ACHKUC_003538"
     CDS             complement(4430..5674)
                     /gene="frsA"
                     /locus_tag="ACHKUC_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6619734.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(5766..6224)
                     /gene="gpt"
                     /locus_tag="ACHKUC_003539"
     CDS             complement(5766..6224)
                     /gene="gpt"
                     /locus_tag="ACHKUC_003539"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6619735.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            6485..7942
                     /gene="pepD"
                     /locus_tag="ACHKUC_003540"
     CDS             6485..7942
                     /gene="pepD"
                     /locus_tag="ACHKUC_003540"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6619736.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(7999..8613)
                     /gene="prfH"
                     /locus_tag="ACHKUC_003541"
     CDS             complement(7999..8613)
                     /gene="prfH"
                     /locus_tag="ACHKUC_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6619737.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLSSGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(8610..9749)
                     /locus_tag="ACHKUC_003542"
     CDS             complement(8610..9749)
                     /locus_tag="ACHKUC_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6619738.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(9939..>10347)
                     /locus_tag="ACHKUC_003543"
     CDS             complement(9939..>10347)
                     /locus_tag="ACHKUC_003543"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6619739.1"
                     /translation="QLCAIFLRAVTMTASQHYSPQQIAAWAQIDESRWKEKLAKSQVR
                     VAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIKSESELTVDASITAKP
                     FFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
BASE COUNT         2575 a         2707 c         2544 g         2521 t
ORIGIN      
        1 acttgagtag acttggggag acgttagaat ggtgccgata ataggagtcg aacctacgac
       61 cttcgcatta cgaatgcgct gctctaccaa ctgagctata tcggccctga aaggacatgt
      121 tcacgaacgt gaatcacggt ggacaaggtt aaaactaacc gggcgatgcg tcaatggcct
      181 tgtgaatcaa atggctactt ttgcatcacc cggttttatt tacgcacgaa tggtgtaatc
      241 accaatgccg atccacttat aagtggtcag tgcttccagc cccattgggc cgcgcgcgtg
      301 gagtttttgt gtgcttaccg caacttccgc ccccagtcca aactggccgc cgtcggtaaa
      361 acgcgtagag gcgttaacgt aaacagcgga ggaatccact tcgttaacaa aacgctgggc
      421 gttgcgcata tcgcgggtca ggatcgcatc ggagtgttgc gtgccgtgtt cacgaatatg
      481 ggcaatggcg tcatccagat cgctaacgat tatgacgttc aaatctaatg acagaaactc
      541 atcgtcatac tcttcggctt taacagccac caccttcgcg gggccagcct gcaactgcgc
      601 cagcgcggct gcatcagcgt gtaacgtcac gccgctttcc gccatttgtt tgcttaatgc
      661 gggcaggaag ctatctgcga tgtttttatt taccagcaac gtttctaccg tattacatgt
      721 gctcggtcgc tgagttttcg cgttgacaat tactttcagg gcttcagcga tctctgcgct
      781 ttcatcaacg taaatatggc atacgcctat accgcctgtg atcaccggga ttgtcgactg
      841 ctcgcggcac agtttatgca aaccagcccc accgcgcggg atcagcatgt cgatgtattt
      901 atccatacgc agcatttcac tgaccagcgc acggtcagga ttatcaattg cctgcacggc
      961 acccgccggt aagccgcagg atttcagggc gtcctgaatc accgctaccg ttgcagcgtt
     1021 agtgcgacag gtttctttgc cgccgcgcag gatcaccgcg ttaccggttt tcaggcacag
     1081 ggaagcaaca tcaaccgtca cgttcgggcg cgcttcataa atcacgccaa tcacccccag
     1141 cggtacgcga cgacgctcaa gacgcaggcc gctgtccagt acgccgccat cgattacctg
     1201 ccccaccgga tcggcgagat tgcacacctg gcgcacatcg tcggcaatgc ctttcagccg
     1261 tgcgggcgtc agtgccagac ggtcaagcat cgcttcgcta aggccattgg cacacgcgtc
     1321 tgcaacatcc tgcgcgttag cgttgaggat actttcgctt tgtgcttcca gttcatcggc
     1381 gattttttcc agcacacgat ttttttcgcg gctggagagt tgcgctaatt tatacgaggc
     1441 ttgcttcgcg gcaatgccca tttgttccag catcggccag ctccttaacg ggtaatcatg
     1501 tcatcacggt gaacggcaac cgggccgtat tcatatccca gtattgcatc aatttcttgc
     1561 gagtggtgtc cggcaatacg gcgtaatgca tcgctgttgt aacgactgac gccgtgggcg
     1621 atatcgcgac cttcgaggtt gcaaatacgg atgacttcac cacgcgagaa attgccagtc
     1681 acgcttttaa tgcctttcgg caacagggag ctgccgcgtt ccagaatggc ggcagttgcc
     1741 ccttcatcta ccgtgatttc acccgcaggc ggcgcaccga aaatccagcg tttacggttt
     1801 tcaagcggag tcgcctgggc atggaacagc gtaccgacgg aaatgccttc catcacatca
     1861 ccaataacgc ccggcttgct gcccgcggca ataatggtgt cgatacccgc acggcaagcc
     1921 acgtcagcgg cctgcaattt ggtactcatg ccgccagttc cgaggcctga aacgctatca
     1981 ccggcaatcg cgcgcagtgc gtcatcaatg ccgtacacat ctttaatcag ttctgcctgc
     2041 ggattgctgc gcgggtcagc ggtatacaaa cctttttgat cggtcagcag caacagttta
     2101 tcggcacccg ccagaatcgc cgccagcgca gaaaggttat cgttatcacc gaccttaatc
     2161 tctgcagtag cgacagcatc gttctcatta attaccggaa cgatattgtt atcgagcaac
     2221 gcacgcaggg tatcacgggc gttcaggaag cgttcacgat cttccatatc agcacgggtc
     2281 agcaacatct gcccgacatg aatgccgtaa attgaaaaca gctgttccca cagttgaatc
     2341 agtcgactct gccctaccgc cgccagcagc tgttttgaag cgatagtcgc tggcaattcc
     2401 gggtacccca ggtgctcacg tccggcggca atcgcacccg acgtcacaat aacaatccga
     2461 tgcccggcgg catgtaactg cgcgcactgg cgaacaagtt caacgatatg ggcacggttc
     2521 aggcggcgcg atccgcctgt tagcacactg gtgccgagtt ttaccaccag cgtctggctg
     2581 tcactcatga ttctctgcca ttcaatttta ggaaaaaatg atatcaaacg aacgttttag
     2641 caggactgtc gtcggttgcc aaccatctgc gagcaaagca tggcgttttg ttgcgcggga
     2701 tcagcaagtg tagcggcagt tgtttacgct tttattacag atttaataaa ttaccacatt
     2761 ttaagaatat tattaatctg taatatatct ttaacaatct caggttaaaa actttcctgt
     2821 tttcaacggg actctcccgc tgaatattcg cgcgttaatt aaaatcagga atgaaaatga
     2881 aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgtatcc gtacaggccg
     2941 cagaaatata taacaaagac ggtaataaac tggatgtcta tggcaaagtt aaagccatgc
     3001 attatatgag tgataatgac agtaaagatg gcgaccagag ttatatccgt tttggtttta
     3061 aaggcgaaac acaaattaac gatcaactga ctggttatgg tcgttgggaa gctgagtttg
     3121 ccggtaataa agcggagagt gatactgcac agcaaaaaac gcgtctcgct tttgccgggt
     3181 tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg ttgtatgacg
     3241 tggaagcctg gaccgatatg ttcccggaat ttggtggcga ctcctcggcg cagaccgaca
     3301 actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac ttcttcggcg
     3361 ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac cgcgacgtta
     3421 aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggt ggcagcgatt
     3481 tcgccatcag tggagcctat accaactctg atcgtaccaa cgagcagaac ctgcaaagcc
     3541 gtggcacagg caagcgtgca gaagcatggg caacaggtct gaaatacgat gccaataata
     3601 tttatctggc aactttctat tctgaaacac gtaaaatgac gccaatctct ggcggctttg
     3661 ccaataagac gcagaacttt gaagcggtcg ctcaatacca gtttgacttt ggtctgcgtc
     3721 catcgctggg ttatgtctta tcgaaaggga aagatattga gggcatcggt gatgaagatc
     3781 tggtcaatta tgtcgatgtt ggtgctacgt attatttcaa caaaaatatg tcagcgtttg
     3841 ttgattataa aatcaaccaa ctggaaagcg ataacaaatt aaatattaat aatgatgata
     3901 ttgtcgcggt tgggatgacg tatcagtttt aatgaatatt gccggatgtg atgcatccgg
     3961 cacatttcat tcacaccgtt aacttcaccg gttcatcacg aaaatcatct gccggttcca
     4021 gcttcagatt cagcgtcgcc agcagctcac gcagcttctc gtgaaattca cgcagggtgt
     4081 gctccagtcg ttcaaccact tcggcatctt ttatctgaac actcgtccag tcgcctgctt
     4141 tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct tccagttcca
     4201 tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg catacagcca
     4261 gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac gggcctagtg
     4321 cggtaaattt tttgactaat ctgctcttcg ggtgtccact cggtaacgtc attgcaatct
     4381 cctgttgtga tgcaactgtt ttaccaaatt ggtaaaattt agcaaatttt taacacaaac
     4441 gtttttcgat ccagtctgtg atttcctgaa ggcctttgtc aaaattccga tacaccgggt
     4501 taaatgggat ctctaataat ttaccgtcag cagatgacga cgtgattaag cgtgagtcct
     4561 cttccgggct gaacggatcg ttcttccagt agcctgataa cattggcgtt gggcagcgac
     4621 gtcccagcaa tccttgcact tttaatgaat agcgattcaa ctccactcgc aacgcttcat
     4681 cggaagcatc atgcatccct aaacgactgg ccagaacgtc aagatacatt tccggcacct
     4741 gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga caggcaacca
     4801 ctttcagacg cggcgattca aggtatgcca gacgcacggc aacgttagcg ccgaaacgga
     4861 aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcagc gcctttaaaa
     4921 cgtgctgatg caacaggctg gagtcctggg tgagcttcca ttttgaagaa aagcccaccg
     4981 acggcatatc aatagtcagc atagcaatgc cgcgcggcgc aaaataacgt tcatacaggc
     5041 tgtaatagtc cgtctgcatc gcatccagac caccacacat taatactgtc gggaacgggc
     5101 catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt acggtaaact
     5161 ccatctgtcg catcgtgccc ggcagacgct gagcggcctc ttcataggcg cggtttgaca
     5221 aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggca gcaatgttgt
     5281 acaacgtagc cgcatgcagc cagtgtcgac cgctgagttg cggatcttct tccgcacagg
     5341 ctttttgttg ccacaccatc gcctgggtag cccactcata aatccagttg ccgccgcggt
     5401 agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc atcacaatac
     5461 gcgcctggac atcgaggatt tcacgggggt caatgccgcg ccagatccac atcagacggt
     5521 taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc acaggcggtt
     5581 gtgcgccgtg actaaagcgg cggactagcg tcgaggtttc aggatgttta aagcggggtt
     5641 tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga atacttactg
     5701 gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc caggcgttga
     5761 aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca cggctgttcg
     5821 atccaggtat tttgcgggat atcaacaaca taatcatcaa ccagcggacg accggccggt
     5881 tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc aaccgcagta
     5941 ccaccggtat ctaccaggtc atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt
     6001 ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca aacggtatcg
     6061 acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc gccacggctt
     6121 acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag tttacgtgca
     6181 tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag tgtcccagcc
     6241 tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag attatagaga
     6301 tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc actgcctctc
     6361 cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct tgtgttgaca
     6421 acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga caaggagact
     6481 taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt ttgccaaaat
     6541 ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca ttgttggttg
     6601 ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc tgattcgtaa
     6661 acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc ttgcaggccc acctcgatat
     6721 ggtgccgcaa aaaaacaacg acaccgtgca tgacttcact aaagatccta tccagcctta
     6781 tattgatggc gaatgggtta aagcacgcgg caccacgctg ggtgcggata acggcattgg
     6841 tatggcctct gcactggcgg ttctggctga cgaaaacgtg gttcacggcc cgctggaagt
     6901 gctgctgacc atgaccgaag aagccggtat ggacggtgct tttggcttac agagcaactg
     6961 gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa tctacatggg
     7021 ttgtgcggga ggtatcgact tcacctccaa cctgcattta gatcgtgaag cggttccagc
     7081 tggttttgaa accttcaagt taaccttaaa aggtctgaaa ggcggtcact ccggcggtga
     7141 aatccacgtt ggcctgggta atgccaacaa actgttggtg cgcttcctgg cgggtcatgc
     7201 agaagaactg gacctgcgtc ttattgattt caacggcggc acactgcgta acgccatccc
     7261 gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgccc tgaaatctct
     7321 ggtggatacc tatcaggaga tcctgaaaaa cgaactggca gagaaagaga aaaatctggc
     7381 attgctgctg gactctgttg cgaacgataa agctgccctg attgcgaaat ctcgcgatac
     7441 ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc cgcaactccg acgtggcaaa
     7501 aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc atgaccgaca ataacgtaga
     7561 aattcactgc ctgatccgtt ctctgatcga cagcggtaaa gactacgtag tgagcatgct
     7621 ggattcactg ggtaaactgg caggtgcgaa aacagaagca aaaggcgcat atcctggctg
     7681 gcagccggac gctaactctc cggtaatgca tctggtacgt gaaacctatc agcgtctgtt
     7741 caacaaaacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc tgttcaaaaa
     7801 accgtatccg gaaatggaca tggtgtctat cgggccaacc atcaccggtc cacactctcc
     7861 ggatgagcaa gttcatatcg aaagcgttgg ccattactgg acactgctga ctgaactgct
     7921 gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt aatcgccttc
     7981 cggcagtttc atccttcatt atccttcgat aaaagccatc cctgtgaacg ttcgtcgcgg
     8041 gttgccacgt tcaatctggt gatggaacat tcgccgctgc gatttcagcg ccgcgctatt
     8101 ttcctgttgc tgttgttcca gcttccaggc aatcagtaat cgcgccagcc gtttgttagc
     8161 atgctgactg cgctctgact gaaccttcac gctaataccg gatgacagat gcgtggcgcg
     8221 taccgccgag tcggttttat tgacatgttg accgcccggc cctgacgaac gcagcgtctc
     8281 ataacggatt gcatccgatt gttcctgctc atcagcggta aaacgcccaa tgcccagaaa
     8341 ccagtttttg cgcccatgat gaggccgata cggactcgga caaatccact gaatagtgcc
     8401 gcaccagctt tcgcttaaag cccatgcgtt atcgccatcc agagaaatca gcgccgaacg
     8461 cagtgtgtca gagtagcgac ccgtttctgt ttccagcacc gttaccgcga cgtcctgtcg
     8521 ggtagcttct ttaatcagcc tgtccagtgc ttttttcact gcgagacaac attcttccgg
     8581 cccctgagca gaggagagtt gtagcaagat catccttttt tccctccact gtttttgagc
     8641 gtcagcaccg ggcgcagtcg cgcgacagga ataattaacc cagccagcac cagacattgc
     8701 accacgcttt cagccgattt ataggcttgt ggcgcttctt caaagatgag ttgtttatcg
     8761 cgacaaatta cccgactgcc aagttcagtc cgcgagagtt gcgtcgccgt gtatttcgct
     8821 gccagacgcc ctttacactc ggtgcgcccc catttacgcc ccgccccatg cgccagcgag
     8881 tggagcgttt tttcgttcgc aacaggttta accagccagg agtaatcgcc gcgtgaaccg
     8941 ggaataatca ccagaccgtt gtcatccggc gtggcgcctt tacgatgcaa ccagccctgt
     9001 tgatcgccga tttggcacgc gctaacaaag ttatgcgcca catccagaac cggacttccg
     9061 gtggccttaa cttgttgcat tatgcgcaaa gcgatcagct ggcgattaat acgcgcaaac
     9121 gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt cgtcactgcc ttcaggcaaa
     9181 ccatgatgcg aaaacgaggc aatatgccgc tgtaaaatag actgccccaa tcccctcgag
     9241 ccgctatgaa ccagcagttg cagatgctgc gcatccagac cagccagtgc aaacagttcc
     9301 gcgtcgataa tttgatcaat ctgttgcagt tctgcgaagt ggttaccgcc accgatggag
     9361 ccaagcgagt tgcgccacgg atgctgtgca aatgctgacg gcaggttttc ctccagccag
     9421 ctttcttcag caacgtcatc cagatctgat aatcgctttt caaacttatc ggcgttgtat
     9481 ttgcgagcga gaatatctgt ttgccatagc gccatgccgc agccgatatc attgccgacc
     9541 agtgccgggt aaaaacgacc aacagagaag aacgctgcgc caatcgggta gccgcgtccg
     9601 ggatgtaaat ctggcatccc cacaacgcgc tgcatgttgg gtaaatttgc cgtggtgtgt
     9661 aattgttgga tcgctaaact ctcgatccac aggtcatcag atgcgatggt aaataccgca
     9721 tcggataagg gacgaatata tttgcccatg taccattcca tttatgaata aaaatcagga
     9781 aatggcagac gaatccaggg caagaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
     9841 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
     9901 acatactaga cgggcgttat tttcattgca agctggattt aatgttgcgg tttatatcgc
     9961 atataaaaat tagtaaacca cgttccccgg cattcaacgc gctgctgctt aactgtctga
    10021 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg cgtccaccgt aagttcggat
    10081 tcagacttaa tcaatggttt taacaaagcg ctggcaaccc cgcgtcgggt gtattccggt
    10141 tcaacaaata acatatcgat ataatgtcca atgcaggtaa taaaaccaac cggttgtgca
    10201 ttaataactg caacccgcac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
    10261 atctgcgccc aggcggcaat ttgctgtgga gaatagtgct gactcgccgt catcgtaaca
    10321 gctcgaagga aaatagcgca tagttgc
//
LOCUS       DAZFRG010000175        10182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFRG010000175 DAZFRG010000000
VERSION     DAZFRG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.175"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1157
                     /locus_tag="ACHKUC_003544"
                     /pseudo
     CDS             1..1157
                     /locus_tag="ACHKUC_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
     gene            1184..2662
                     /locus_tag="ACHKUC_003545"
     CDS             1184..2662
                     /locus_tag="ACHKUC_003545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6619740.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSEN
                     AAVMEKFNIPRQMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            2827..3747
                     /locus_tag="ACHKUC_003546"
     CDS             2827..3747
                     /locus_tag="ACHKUC_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6619741.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGE
                     SAFIGKVGDDPFGRFMYQTLSKENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPTFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            3915..5432
                     /locus_tag="ACHKUC_003547"
     CDS             3915..5432
                     /locus_tag="ACHKUC_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6619742.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            5493..6863
                     /locus_tag="ACHKUC_003548"
     CDS             5493..6863
                     /locus_tag="ACHKUC_003548"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO6619743.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            6863..8272
                     /locus_tag="ACHKUC_003549"
     CDS             6863..8272
                     /locus_tag="ACHKUC_003549"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6619744.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDFFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQQGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            8298..9305
                     /locus_tag="ACHKUC_003550"
     CDS             8298..9305
                     /locus_tag="ACHKUC_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6619745.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(9390..10061)
                     /gene="queE"
                     /locus_tag="ACHKUC_003551"
     CDS             complement(9390..10061)
                     /gene="queE"
                     /locus_tag="ACHKUC_003551"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO6619746.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         2594 a         2330 c         2519 g         2739 t
ORIGIN      
        1 gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg gccattattt
       61 tctatgcccc tagcggcgtt attgccgata aattttcaca tcgcaaaatg attacttccg
      121 cgatgatcat taccggatta ctgggtctgt taatggcaac atatccaccg ctgtgggtaa
      181 tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga
      241 ttaaagccgc atcgttgctg ggcgatcata gcgagcaagg gaaaattatg ggctggatgg
      301 aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc
      361 gctttgcacc ggatgacagc gccagcctga aaacggtcat tattatctat agtgtggttt
      421 acattctgct gggcattttg tgttggtttt ttgttagcga taacaacaat ctgcgcagtg
      481 ccaataacga agaaaaacag tcattccagc ttagcgacat cctggccgtt ttgcgcatca
      541 gcaccacctg gtattgcagc atggtgattt ttggtgtctt caccatctac gccattctga
      601 gttactccac caactatctg accgaaatgt acggcatgtc gttggtggcg gcgagctaca
      661 tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttggc ggcattatca
      721 ccacctacag taaagtgaaa tcccctaccc gcgtggtcca aatcctttcc atcatcggtt
      781 tgctggcgtt gacagccctg ctcgtcacga actctaaccc gcaatcggtc gcgatgggga
      841 ttggcttgat tttattactg ggattcacct gttacgcctc acgcgggctg tattgggcct
      901 gccctggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg
      961 tgattggatt cctgccggat gtcttcgttt aaccgattat cggctactgg caagacaccc
     1021 tgcccgctgc agaagcctac cgcaacatgt ggctaatggg catggcggcg cttggcatgg
     1081 tgatcatctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa
     1141 tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat
     1201 catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa
     1261 tgtggtttgc gaaggcaaag gcttattgca gccgatgcac acgccagatg ccgataccgc
     1321 agaacatcct gacgacgatt tatgggcatc actatgtttt gccagtcacg atttgatgag
     1381 ccagtttgcc ggaaataaag aagatattgt cggcattggc ctgggctcaa tccgttgctg
     1441 ccgtgcgtta ttgaaagccg atggtacgcc agccgcgccg ttgattagct ggcaggatgc
     1501 acgcgttaca cgcccttacg aacacactaa tcctgacgtg gcctatgtca cctctttttc
     1561 gggttatctg acacatcgct taaccggcga gtttaaagac aatatcgcca actattttgg
     1621 tcagtggccg gtggattata agagctgggc atggagcgaa aatgccgcgg taatggaaaa
     1681 gtttaatatc ccccgccaga tgctttttga cgtgcaaatg ccaggcactg ttcttgggca
     1741 tatcacacca caagctgcgc tggcgacaca tttcccggct gggctgccgg ttgtttgtac
     1801 caccagtgat aaaccggtag aagctctggg agccgggtta ctggatgatg aaacggcggt
     1861 aatttctctt ggcacctata tcgcgttgat gatgaacggc aaagcactgc cgaaagatcc
     1921 ggtcgcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg
     1981 tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat
     2041 tcaggatgcc aaagcacagg atctttcacc ggaagatttg ctcaacaaaa aagcgtcttg
     2101 cgtgccacct ggttgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga
     2161 accgtacaaa cgcggaatta tgatcggctt tgattccagt atggattacg catggatata
     2221 tcgttcgata ctggaaagcg tggcgctgac gctgaagaat aattacgaca atatgtgtca
     2281 tgaaatgaat cactttgcaa agcatgtgat cattactggc ggcggttcga acagcgatct
     2341 gtttatgcag atttttgccg acgtgttcaa ccttccggca cgccgaaacg ccattaacgg
     2401 ttgtgcaagt ctgggggctg cgatcaatac ggcggtaggt ctggggctat acccggatta
     2461 cacaacggct gtcgataaaa tggttcgcgt gaaagatatc ttcataccga ttgagagcaa
     2521 tgccaaacgc tacgacgcga tgaataaagg cattttcaaa gacctaacca aacatactga
     2581 tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatg cggattcgat
     2641 ccagagctgg tcgaatgcgt aagtgagata aacagtgccg ggtagatgat taatcccggc
     2701 atattcacgt ttccactaca aaataccggg taaccactgc tgaatattgt tctctcctga
     2761 gcagcgttta tgctttaatt gctaaaccga tttaatttgt ttagcagtta gtatattgga
     2821 gcctttatga gcacaagagt atgggtactc ggtgatgcgg ttgttgattt attacccgaa
     2881 agccagggga gactactaca gtgtcctggt ggggcgcctg ctaatgttgc agtcggtatc
     2941 gcaaggctgg ggggggaaag tgcctttatt ggcaaagttg gcgatgatcc tttcggtcgc
     3001 tttatgtatc agacactgag taaagaaaat gttgatacac gttatatgtc tcttgatcct
     3061 caacatcgca cctcaattgt ggctgtagga cttgatgagc aaggagaaag aaactttacc
     3121 tttatggtac gcccaagtgc cgatcttttt ttacaacctg atgaccttcc tacatttggg
     3181 ccgggtgaat ggctccatct ttgttccatt gcgctcagtg cagaaccttc ccgaagtacc
     3241 gcatttctgg ctatggagaa aatacgtcag gctggcggaa acatcagttt tgatcccaat
     3301 atccgcagcg atctctggca gagtgaagcg ctattaagaa aataccttga tcgcgcgctt
     3361 tcgctggcga atatcgctaa attgtccgaa gaagagttgc tattcatcag tggcgaaagc
     3421 caggttcagc aaggcgcata ttcattagta caacgttatt cgttgacttt attgcttatc
     3481 acacaaggaa aaaatggcgt acttgtgtat tttcaggggc agtttatcca ctatcccgcc
     3541 aaacctgttt ctgtcgtcga tacgaccggg gcaggagatg cttttgtcgc tggattactt
     3601 gcaggtctgg ctgattctgg aataccaaca aataccagac agcttgaacg aatcattgca
     3661 caagctcaga tttgtggtgc tctggcgacc acggctaaag gcgcgatgac cgccttaccc
     3721 cgacaacacg atctcccttc acaatagtga tcctgctgtt gtatctgtaa cccgatttgt
     3781 cgtgcctgtc acatttacta aatcggttta gcgaaaataa ttgctctggc taaaaagtca
     3841 gctttattat caatacctaa accggtttag cattttagca atttttataa ttctctgatg
     3901 ggacgcgaca aattatgtat aaaaaaacaa ctttggcagt gttaattgct ttgctgaccg
     3961 gtgctacaac ggtacatgcg caaacggata ttagcagtat cgaatctcga ctggcggcgt
     4021 tggaacaacg tttaaaaaat gcggaatccc gcgcccaggc ggcagaagca agggccaaaa
     4081 cagctgaatt acaggttcag aaactggctg aaacacaaca acaaaatcag ctaacaactc
     4141 aagaagtagc acagagaaca gttcagctcg aacagaaatc cgcagaaaac agtggttttg
     4201 agtttcatgg ctatgcccgt tccgggttac tgatgaatga tgccgcttcc agtagcaaaa
     4261 gtgggccgta tctgactccc gcaggtgaaa ctggtggagc tgttggccgt ctgggaaatg
     4321 aagccgatac ctatgtcgag ttaaatgtag aacataaaca aacactggat aacggtgcga
     4381 ccacacgctt taaagcaatg ttggctgacg gacaaagaga ttacaacgac tggactggcg
     4441 gctccagtaa cctgaatatc cgacaggctt ttgccgaact gggcgcatta ccaagtttta
     4501 ccggagcatt caaagacagt actgtctggg ctggtaaacg ctttgatcgc gacaattttg
     4561 atattcactg gttagactcc gatgtcgtat ttttagcggg aacgggcggc ggtatctatg
     4621 acgtaaaatg gaacgataca ttccgcagta acttttctct ctacggacgt aatttcggcg
     4681 atcttgatga tatcgacaat aacgttcaga actacatcct caccatgaat cattatgcag
     4741 gccccttcca gttgatggtt agcggattac gggcaaaaga taatgatgat cgaaaagatg
     4801 ccaatggtga tctcattcaa actgatgctg caaatactgg cgtacatgcg ttagttggtc
     4861 tgcacaatga cactttctat ggcctgcgtg aagggacggc aaaaacagca ctgctatatg
     4921 gccatggcct gggtgcggaa gtcaaaggga ttggctccga tggcgctctg ctgtctgagg
     4981 cgaatacctg gcgcttcgca tcttacggca caacacctct gggaagcggt tggtatgttg
     5041 cgccagcaat tctcgcacaa agcagtaaag atcgttacgt caaaggcgat agctacgaat
     5101 gggtgacctt caatacacgt ctgatcaaag aggtaacaca gaattttgct ctggcctttg
     5161 agggtagcta tcaatatatg gatctgaagc caaaggggta tcaaaaccac aacgccgtaa
     5221 acggcagctt ctataaactc acctttgctc caactctaaa agctaacgat atcaataatt
     5281 tctttagccg tccggagctt cgcctgtttg ccacctggat ggactggagc agcaaacttg
     5341 atgattttgc cagcaatgac gctttcggca gcagtggttt caatactggc ggagagtgga
     5401 attttggtgt ccaaatggaa acctggtttt aataccagac agcctcacgg atgtgaggct
     5461 tttactgaaa taacaatgaa ttagagggtt atatggattt cgataaaatc gcccaatcgt
     5521 tgcttccttt attaggtggt aaggagaaca tcgccagtgc agcacattgc gccactcgcc
     5581 tacggctggt actggttgac gacacacttg ccgatcaaca tgccattggc cagattgatg
     5641 gagtgaaagg ttgttttcgt aattcagggc aaatgcaaat catctttggc accggcgtgg
     5701 ttaataaagt ctatgctgcc tttattcagg ttgcaggtat tagcgaatcc agtaaagcag
     5761 ataccgctcg actcgccgct caaaaactca atccttttca gcgaatagca cggctacttt
     5821 ctaatatttt tgtccccatc attccagcca ttgttgcttc tggtttatta atggggctcc
     5881 tgggaatggt gaaaacatat ggctgggtga atgccgataa tgcgatttat atcctgctgg
     5941 atatgtgcag ttcagccgca tttattattc tccccatcct tattggcttt actgctgctc
     6001 gtgaatttgg tggaaatcct tatcttggcg cgacattagg agggattctg actcacccgg
     6061 cactcacaaa tgcctggggc gttgctgcgg gatttcagac aatgaacttc ttcggttttg
     6121 aaatcgccat gattggctat cagggaacgg ttttccccgt gcttctggca gtatggttta
     6181 tgagcatagt ggaaaaacag ttacgcaggt ttatccctga tgctctggat ctcattctga
     6241 cgccatttct gactgtcgtc atttctggct ttatcgctct tttgattatt ggtccggcag
     6301 gacgagcgtt aggtgacggt atctctttcg ttctcagcac gctgattgca cacgcaggct
     6361 ggttagcagg gttgttattc ggcgggctat attcagcgat tgttattacg ggtattcatc
     6421 atagcttcca cgcaattgaa gcaggacttt taggtaaccc tgcaatcggt gttaatttcc
     6481 tgctgcctat ttgggcaatg gcaaacgttg cgcaaggcgg tgcatgtctg gcggtatggt
     6541 ttaaaaccaa agatacaaaa attaaagcaa tcaccctacc ctcggccttt tccgcaatgt
     6601 tagggattac tgaagccgct atttttggta tcaaccttcg ttttgttaaa ccttttattg
     6661 cggccttaat tggtggcgct gccggcggtg cctgggttgt ctctgttcac gtttatatga
     6721 ctgccgttgg tctgaccgca atacccggca tggcaatcgt tcagccaaca tcgttggtta
     6781 attatattat tggaatggtg attgctttcg ctgtcgcttt tagcctctct ttattactca
     6841 aatacaaaac ggacgaggag taatgtcgat gccttcacaa atgcccgcga ttttacaggc
     6901 agtgatgaaa ggtcagtcca aagcactggc agatgcccac tatccttgtt ggcatctcgc
     6961 tccagtcacc ggactaatga acgatccaaa tggcttttgt tggtctggag gacgctatca
     7021 tctgttttat caatggaatc cactggcttg tgatcataag tacaaatgtt gggggcactg
     7081 gagttcgacg gatttattgc actggcagca tgaaccatta gcgctaatgc ccgataaaga
     7141 gtacgaccgt aatggttgtt actcaggaag cgcggttaat aatcagggcg ttctcacact
     7201 ttgctatacc ggtaatgtta agtttgatga tggcagcaga acggcatggc aatgcctggc
     7261 caccgaaaat aaccagggcg ggtttgataa actaggccct gtcataccgt tacctgatgg
     7321 ttacaccgga catgttcgtg atccaaaagt ctggaagcac aacagccaat ggtacatggt
     7381 gcttggcgcg caagataaag agaaacgggg gaaagtgctg ctgtattcct ctgtcgatct
     7441 ctatacctgg tcttttcatg gagaaattgc tggtaacggt ctgaatgaaa tagataacgc
     7501 aggatacatg tgggaatgtc ctgatttttt cgcccttgat ggtgaataca ttctcctctg
     7561 ttgcccccag ggaatggtgc gcgaacatga gcgctatctg aatacttatc cctgcgcctg
     7621 gctacatgga cagtttgatt acgagacagg caaatttacg catggcgctt tctcagagct
     7681 ggatgcggga tttgaattct atgcaccaca gacgatggaa gccccggacg ggcgacgatt
     7741 gctggtcggc tggatggggg ttcctgatgg cgaagagatg ttgcagccaa ccagaaagca
     7801 tcattggcag catcaaatga cctgttttcg cgaactctca ttccagcagg gaaaattgtt
     7861 tcagatgcca atcagggaac tcgcgcaact gcgtgaagct gaacacttct ggcaaggaaa
     7921 agcggatcat gcgccgcatg tcgaaattga acgcctggaa atggacatca ttccgtcggg
     7981 tgagctgtat ctgaattttg gcaatgcgct ggcactgcat cttaatgacg atggtattca
     8041 actacagagg agaagccttg cagggcaaga gaaattaact cgttactggc gaggaagcgt
     8101 aacgtcactg aagattctct gtgacagttc cagcgtagag atattcatca ataatggtga
     8161 aggtgtaatg agtaatcgtt attttcccca tcatccagct tcgctgatat tacagggtga
     8221 gtctgacgtc acgttacgct actggtcgct acgcgcctgc atggtagaat gagggttttg
     8281 aattacggat cctcgtcgtg agaaaaacca aacgcgttac tattaaagac attgctgaac
     8341 tggcgggagt gtcgaaagca accgccagtc tggttctcaa tggtcgtggc aaagagttac
     8401 gtgttgcgca ggaaacgcgt gagcgtgtgc tgagtattgc ccaacagcag cattatcagc
     8461 ccagcattca tgcccgctca ttacgggaca accgcagtca taccattggt ctcgtcgttc
     8521 ctgaaatcac taactacgga tttgcagtat tttcccatga actggaaaca ttgtgtcggg
     8581 aagcgggcgt ccagctcctc atatcctgta ctgatgaaaa tccttcacag gaaacaatgg
     8641 tggtgaacaa tatgattgcc cgccaagttg atgggttaat cgtcgcatcc agtatgctgc
     8701 atgataatga ctatcaaaaa cttagcgagc aattacctgt tgtgttgttc gatcgccaca
     8761 tgaacggcag tacattgcca ttggtcatta ctgactctgt ttctcccacc gccgctctgg
     8821 tggcagatat tgcacggagt cacccggatg aattctattt tctgggcgga caaccccgtt
     8881 tgtcaccgac tcgcgatcga ctggaaggat ttacacaagg cttgcagcaa gccggagtaa
     8941 cgctacaacc tgaatggatt attcacggta attatcatcc gagctcaggc tatgaaatgt
     9001 ttgccgcgct atgcgctcgt ttaggacgtc cccctaaagc cttattcact gccgcttgtg
     9061 gattactgga aggcgttctg cgctatatga gccaatataa tttactcgac agtaaaatac
     9121 acctggcgag ttttgatgat cattacttgt atgattcact ttcggtaaga atcgacacaa
     9181 tacaacagga taatcgccaa cttgcttttc actgctttga acttatttcg caactgattg
     9241 aaggtgaaac gccatcccca ttacagcgtt atcttcctgc cagtctacaa aaacgctacc
     9301 gataatcccg tcactatctt gcatcgcccc tgtaaaacgg ggcgattatt ttcatcctga
     9361 acgttaaata ttaatgatga ataatcaaat caggcaatat tcaaatattt atgtgtctgc
     9421 atcgacaaac gccagttgcg cgcaatgcag gtatcgatac ataaacgcgt agcatcttcc
     9481 ttctggctaa ttggctgaag tgcaatgact cgcggtttat catcggtcag cgtcgccagc
     9541 aattcgtcca gtgcttcgat atcacgtacg cgtccaactg ggtgtttgat ttcgttagct
     9601 cgctccaggg cttgtgacaa cacttcatag ccgccgcgca tgttcagctt tggcgatacg
     9661 gtaacccagg tattcggtgt gcagcgtacc tcatgagtac cactggtttc gatctggcag
     9721 ctaaaaccgt tcttttcgag caggtcagtc agtggcagca aatcatgaat gcaaggctca
     9781 ccacccgtaa tcaccacatg acgcgcggtg tatccctgac ggccaatgac agccagcaaa
     9841 tcttcactgc tcgccgcccc ccacttatca ctctctttgg tcttcgccag aatgctgaaa
     9901 agggagactt cccgatcctc aagcttttcc caggtgtgtt tggtgtcgca ccaggcacag
     9961 ccaaccggac atccctgtaa acgaataaaa atggcgggga cgccggtaaa gtaaccctca
    10021 ccttgcaggg tctggaacat ctcgttaatc gggtactgca tagcattctc tgtgaagtgg
    10081 ataattgtta attattgcag atcctgccac aacaatcatg tcttattagc attctgttac
    10141 aggcaggttt gatgcaaaaa ttggctcaga tcattaagaa ta
//
LOCUS       DAZFRG010000176        10158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFRG010000176 DAZFRG010000000
VERSION     DAZFRG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.176"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..349
                     /gene="hokA"
                     /locus_tag="ACHKUC_003552"
     CDS             197..349
                     /gene="hokA"
                     /locus_tag="ACHKUC_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO6619747.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(326..397)
                     /locus_tag="ACHKUC_003553"
     CDS             complement(326..397)
                     /locus_tag="ACHKUC_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619748.1"
                     /translation="MRNTVRKAGIISRRRRLLFQFAG"
     gene            complement(537..749)
                     /gene="cspA"
                     /locus_tag="ACHKUC_003554"
     CDS             complement(537..749)
                     /gene="cspA"
                     /locus_tag="ACHKUC_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO6619749.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1030..1320)
                     /gene="yiaG"
                     /locus_tag="ACHKUC_003555"
     CDS             complement(1030..1320)
                     /gene="yiaG"
                     /locus_tag="ACHKUC_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO6619750.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1754..2464
                     /gene="yiaF"
                     /locus_tag="ACHKUC_003556"
     CDS             1754..2464
                     /gene="yiaF"
                     /locus_tag="ACHKUC_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO6619751.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2514..3488)
                     /gene="ghrB"
                     /locus_tag="ACHKUC_003557"
     CDS             complement(2514..3488)
                     /gene="ghrB"
                     /locus_tag="ACHKUC_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO6619752.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLRPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3592..4251)
                     /gene="yiaD"
                     /locus_tag="ACHKUC_003558"
     CDS             complement(3592..4251)
                     /gene="yiaD"
                     /locus_tag="ACHKUC_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO6619753.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTSEGKAQNRRVEITLSP
                     L"
     gene            4404..6737
                     /gene="bisC"
                     /locus_tag="ACHKUC_003559"
     CDS             4404..6737
                     /gene="bisC"
                     /locus_tag="ACHKUC_003559"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO6619754.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6706..7146)
                     /gene="yiaC"
                     /locus_tag="ACHKUC_003560"
     CDS             complement(6706..7146)
                     /gene="yiaC"
                     /locus_tag="ACHKUC_003560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6619755.1"
                     /translation="MIREVQRSELPAILQLWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7143..7706)
                     /gene="tag"
                     /locus_tag="ACHKUC_003561"
     CDS             complement(7143..7706)
                     /gene="tag"
                     /locus_tag="ACHKUC_003561"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO6619756.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7864..8562
                     /gene="yhjY"
                     /locus_tag="ACHKUC_003562"
     CDS             7864..8562
                     /gene="yhjY"
                     /locus_tag="ACHKUC_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6619757.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8791..9993
                     /gene="yhjX"
                     /locus_tag="ACHKUC_003563"
     CDS             8791..9993
                     /gene="yhjX"
                     /locus_tag="ACHKUC_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6619758.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2442 a         2420 c         2602 g         2694 t
ORIGIN      
        1 gattatatca aggagaagaa acgaacttat taagctagaa tagccacggg tgcttgagac
       61 tgtttgtctc aggtattcac cgaaaggcag acagagaaaa gccccacctg actataaatc
      121 aaagtgaggc ccctctatgc atagacacca taaggttagc ctcttactcg ttggaaatca
      181 acacaggggg ctgggaatgc cgcagaaata tagattactt tctttaatag tgatttgttt
      241 cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc atattaaaca
      301 ggagagttat gagctggcgg cgtttttagc ctgcaaattg aaagagtaag cgtcttcggc
      361 gggaaattat tcccgccttt cttacggtgt tgcgcattct cattgcaccc aaatttattc
      421 ttcacaaaaa taataataga ttttattacg tgatcgatta tttatttcct gaaaaacaaa
      481 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
      541 aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
      601 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
      661 acgtctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
      721 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
      781 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
      841 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
      901 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
      961 tttatgatta atcggttgga aaacggtcct gttatcagga ccgtaaacag caataaagtg
     1021 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
     1081 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
     1141 gactgatacg cccaaaaccc gggcaaaatc atcgattttt aatcctgtcc cttttcgtaa
     1201 ctgctcaatt tcggtacagg atgttgttct gtgcgtcagg gtaattttct gcgtttcatc
     1261 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
     1321 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
     1381 tcgggaaaaa cagacacagc ggggtgttgt cagggcatac tctgactatg attcgattca
     1441 gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
     1501 taccactccc ggtttgtaaa gatttcgaaa atctaacgtt ctgcgaaggc gtaaatctct
     1561 ggctacaaaa aaagcaacgg caatatcatt ttttaacaat gaaacaacaa attaaccttg
     1621 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     1681 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     1741 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     1801 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     1861 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
     1921 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     1981 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa
     2041 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaacgg
     2101 ttcgctgggt gtgctggcgc aacagctgca aaatgcgaag ttgcaggccg atgctgcaca
     2161 ctctgcgtta aaacagagtg atgacctgaa accggttttt gatcaggcat tcaccaaagt
     2221 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
     2281 acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc
     2341 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     2401 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     2461 ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc
     2521 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
     2581 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     2641 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg
     2701 aagacatcca gcccggcggc gtgaatttcc cctttctgca atgcagcaat cagtgcattt
     2761 tcatcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     2821 aattgttctg cgccaaacag atgatgcgtc tcatccgtta acggcaggat caggcaaacg
     2881 aaatctgact cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     2941 gcttctttat ggtggcgacg tgcgttatag aggatgggca tgttgaagcc aaagtgcgca
     3001 cgttgtgcca gcgccatgcc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     3061 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     3121 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     3181 acggtttctg ttaatacggt tggtgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     3241 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
     3301 ttttccagca atgcggcatc aacattctcg tttgaaccca gcaagccttc agcttcggca
     3361 aaaattgctg cattttgttc gacggtttgt gggcggaggt ttgccacctg gtgaacggtg
     3421 aaatgttctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     3481 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     3541 ggaaaactga agaatgccag gtgatttctg catcacctga catgaaaggg attacagtgg
     3601 gcttaaggta atttctacac ggcggttttg cgccttacct tctgaggtgc tgttgctggc
     3661 gattgggtta gccgggccaa ggccttgagt acggatgcgg ctggcgtcca cgccctgggt
     3721 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg
     3781 actccccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     3841 taccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg cgctgctgct
     3901 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     3961 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     4021 aacgccgccg cccagagctg cgcctgctgc tgcgccaatg agcgcgcctt taccgcgatc
     4081 tttcttcgaa gaagagagcg caccaatacc cgcgccaacg agagagccca gacctgcgcc
     4141 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtac agccagatac
     4201 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
     4261 ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgagca
     4321 tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat
     4381 ctaacctttc aggagtcttc ggtttggcca actcatcctc acgatattcc gttctgactg
     4441 ccgcccactg ggggcctatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg
     4501 gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca
     4561 gcaatacgcg ggtgcgattt ccaatggtgc gcaaaggctt tcttgcgtca ccggaaaatc
     4621 cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggagc
     4681 ttattcacca tcaacataaa cgcattcgtg aggcttatgg cccggcatcg atttttgctg
     4741 gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct
     4801 atatggcgct ggcaggcggt tatactgggc atctggggga ttattcgacc ggcgcggcac
     4861 aggcgatcat gccgtatgtc gtgggtggca gcgaagttta tcaacagcaa accagttggc
     4921 cgctggtgct ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc
     4981 tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg
     5041 acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccatc gatttctttg
     5101 gcgataaaat ggagtgggtg gcaccacaca tgggcaccga tgttgcgttg atgctgggga
     5161 tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca
     5221 caggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgacggtata gcgaaaaacg
     5281 ccgaatgggc ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta
     5341 ttttccacca aaatacaacc atgctgatgg caggctgggg aatgcagcgc caacagtttg
     5401 gtgagcaaaa acactggatg atcgtcacgc tggcggcaat gttggggcaa atcggcacac
     5461 ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcgccgcg
     5521 ctgcggtgct ctcttccatg cagggtagct tgccgggtgg caccgatgcg gtagataaaa
     5581 tccctgtcgc ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacataacg
     5641 gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggtggt gccaacttta
     5701 ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag ctggtggtga
     5761 tctccgaatg cttctggacg gctgcggcga aacacgcgga tatcgttctg cctgcgacaa
     5821 cctcttttga gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc
     5881 cgatgaagca agtggtgccg ccacgcgatg aagcgcgtaa tgattttgat gtttttgccg
     5941 acttgagtga acgttgggag aagggcggtt atgcacgttt tacggaagga aaaagtgagc
     6001 tgcaatggct ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg
     6061 aattgccgcc atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc
     6121 cggacagtga gcggtttatt cgcttcgccg attttcgccg cgatccgcag gcgcatccat
     6181 taaaaaccgc cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc
     6241 cggattgccc ggggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac
     6301 cggaacagtt gcaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt
     6361 acagttctct gcgcgaattg tacgcggtgg caaatcgtga gcctgtcacc attcatcctg
     6421 acgatgccca gacgcgcggc atacaagatg gcgatactgt tcggctgtgg aactcacgcg
     6481 ggcaaattct tgccggcgcg gtcattagcg agggaataaa acctggcgtg atttgtattc
     6541 acgaaggcgc atggccagat ctggatttaa ccgctggcgg catttgtaaa aacggcgcag
     6601 tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata
     6661 cggcgctggc atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac
     6721 caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac
     6781 aatgtgaaaa ccctgcgcat ggtagaaatc tatcgccggt tgattttttt gataaacctc
     6841 cagcatcaga tggggatggc gctgctgcac atactgcatc agcgccttac caataccgcg
     6901 cctgacggcc ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac
     6961 aaaaccgaga agcttaccgt cttcttccca gacccagttt tgcgcgttgg caagatacgc
     7021 atcccgcacc agcggaatgc agtcacgcca gtaactcgat tttataaagg gatgccccca
     7081 ggttgtactt tccagccaca gttggaggat cgcggggagt tctgaacgtt gcacttcccg
     7141 aatcatggtt tatttcccgg atggcaacag cagccaacca catgatcatt caccagccca
     7201 catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttt
     7261 agtgccttag atagggcgtc ggaggcgggt gtagatgtgg ggatttcgct caacgttgtg
     7321 gcttgtgtca cctgtggttg atgattgaca aacgaccaga caaagtcggc aaacggttcg
     7381 ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttccca
     7441 cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgcg
     7501 gcaactttca ccggatcgaa ctgatgaaag catgcgcgat agttttcgcg ttttttgagg
     7561 acggtgatcc acgataatcc agcctgctgc ccttccaggc agatcatttc gaacagtttt
     7621 ttactgtcag tttcaggcac gccccattca ttatcatggt aggcaatata aagcgggtcc
     7681 tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtgcccgat cgtaaaaatt
     7741 tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc
     7801 ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg
     7861 ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgcttgc gagcgtggta
     7921 atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg
     7981 caacccggac attccacaga gcgttacacc tgggatagcg atcatcaacc tgattacaac
     8041 gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg
     8101 gcggaagaaa cccctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta
     8161 tatgagcagg ttacaaccgg cccggtcgcg gcattacatt acgatggcac aactacctcg
     8221 atgtataacg agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc
     8281 gtgagtacct taggctggcg tgttgactcc cggcttggcg atcttcgacc ctgggcgcaa
     8341 atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg
     8401 atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg
     8461 ctcaatcaaa atattgctgc ctatgctgcg ctatctcagg cagaaaatac cactaacaat
     8521 agcgactatc tgtatacgat gggggttagc gccagatttt aacgttatag atacaattga
     8581 aacccataaa taacaactaa tgtggcgata gtggggacta acttactaaa taataaattt
     8641 ggcgaataat tgtcgagtca ttcattcctg aactagtgca tttcattccg ttctgatggc
     8701 atttcatgcc gtttttcccc aggcataaag cgcacttcgt tatggttgtc agcagagatt
     8761 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtactcgc
     8821 tggctgacac tcatcggtac tatcattacc cagtttgctc tggggtcggt ttatacctgg
     8881 agtctgttta acggtgcgct ttctgccaag ctgggggctc ctgtaagcca ggttgctttc
     8941 tctttcggct tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag
     9001 gaacgttttg gtgttaaacg cgtcactatg gcttccggca ttttgctggg attaggtttc
     9061 ttcctgacag cacattccaa caacctgatg atgctgtggt taagcgccgg tgtgttggtt
     9121 ggtctggcgg atggtgcggg ttacctgttg acgctctcta actgcgtgaa gtggtttccg
     9181 gagcgtaagg gacttatctc tgcttttgct atcggttctt atggtctggg cagcctgggt
     9241 tttaaattta tcgacacgca cctgctcgaa acggtgggtc tggaaaaaac ctttgttatt
     9301 tggggcgcga ttgtactggt gatgattgtc tttggcgcaa cgttaatgaa agatgcgccg
     9361 aagcaggaag tgaaaaccag caatggtgtg gtggagaagg actacaccct ggcagagtcg
     9421 atgcgtaaac cgcagtactg gatgttagcg gttatgttcc tgactgcgtg catgagtggt
     9481 ctgtatgtga ttggtgtagc gaaagatatc gctcaaagtc tggcgcatct tgatgcaatt
     9541 tccgcagcca atgctgtgac ggttatttcc atcgccaacc tttctggtcg tctggtgctg
     9601 ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtgattgcg
     9661 ctggtgggga tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg
     9721 attgcctgtg tggcatttaa ctttggtggc acgattactg tgttcccgtc gctggttagt
     9781 gagttcttcg gcctcaataa cctggcgaaa aactacggcg tcatttacct cggtttcggt
     9841 atcggcagca tttgtggttc gattatcgct tcactgtttg gcggattcta tgtgactttc
     9901 tacgtgattt tcgccctgct gattctgtca ctggcgcttt ctacgaccat tcgccagcca
     9961 gagcagaaaa tgttgcgtga ggcacatatt tagaaatatg ttgctgctga aataaatacg
    10021 atgagggaga cttcgaagtc tccctttttt tatggttgca tgttaaaaga acaatggcat
    10081 ccaaaaaaat gaatattttt cgtttataca ttctttatgt aaatataaat attttcttaa
    10141 aaaaatggta attaaatt
//
LOCUS       DAZFRG010000177        10078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFRG010000177 DAZFRG010000000
VERSION     DAZFRG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.177"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..1397)
                     /gene="pgtP"
                     /locus_tag="ACHKUC_003564"
     CDS             complement(33..1397)
                     /gene="pgtP"
                     /locus_tag="ACHKUC_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter PgtP"
                     /protein_id="HFO6619759.1"
                     /translation="MLSIFKTGQAADSVPAEKIQVTYRRYRMQALLSVFLGYLAYYIV
                     RNNFTLSTPYLKEQLDLSATQIGVLSSCMLIAYGISKGVMSSLADKASPKVFMACGLV
                     LCAIVNVGLGFSTAFWIFAALVILNGLFQGMGVGPSFITIANWFPRRERGRVGAFWNI
                     SHNIGGGIVAPIVGAAFALLGSEHWQSASYIVPACVAIVFAVIVLILGKGSPHQEGLP
                     SLEEMMPEEKVVLNTRQTVKAPENMSAFQIFCTYVLRNKNAWYVSLVDVFVYMVRFGM
                     ISWLPIYLLTVKHFSKEQMSVAFLFFEWAAIPSTLLAGWLSDKLFKGRRMPLAMICMA
                     LIFICLIGYWKSESLFMVTIFAAIVGCLIYVPQFLASVQTMEIVPSFAVGSAVGLRGF
                     MSYIFGASLGTSLFGIMVDHIGWHGGFYLLGCGIICCIIFCWLSHRGAIELERHRAAY
                     IKEH"
     gene            1796..3013
                     /gene="pgtC"
                     /locus_tag="ACHKUC_003565"
     CDS             1796..3013
                     /gene="pgtC"
                     /locus_tag="ACHKUC_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transport regulator PgtC"
                     /protein_id="HFO6619760.1"
                     /translation="MILLLLLMDVGAVRAQRNELVMATTFSPGAIAWIIQRWQTEPES
                     VMIRTLNRTSASLEQLLDTVNVENVDLILTSSPMLLQHLQEHQKLAPFDDAPAESQNL
                     VPESIRATSVAVAISGFGLLINRPALSVKHLPAPADWDDLALPIYQDALLMSSPSRSD
                     TNHLMVESLLQQKGWVKGWETLLTSAGNLVTISSRSFGVADKIKSGLGVAGPVIDNYA
                     NLLLNDPHISFTYFPRSAVSPTYVAILRKSPHADAARRFIHYLLSPKGQRILADANTG
                     KYPVTPLAADNPRATQQQLLMNQPPLNYHLILKRQRLVQRLFDTAISFRFAQLKDAWR
                     ALHSTEARLKKPLPEIRALLTQMPVAAASSEDPVWLAQFDNKSFAEQQMMEWQLWFLN
                     NQRLAIKKLEELK"
     gene            3010..5019
                     /gene="pgtB"
                     /locus_tag="ACHKUC_003566"
     CDS             3010..5019
                     /gene="pgtB"
                     /locus_tag="ACHKUC_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PgtB"
                     /protein_id="HFO6619761.1"
                     /translation="MKNITLWQRLRQVSISTSLRCAFLMGALLTLIVSSVSLYSWHEQ
                     SSQIRYSLDKYFPRIHSAFLIEGNLNLVVDQLNEFLQAPNTTVRLQLRTQIIQHLDTI
                     ERLSRGLSSRERQQLTVILQDSRSLLSELDRALYNMFLLREKVSELSARIDWLHDDFT
                     TELNSLVQDFTWQQGTLLDQIASRQGDTAQYLKRSREVQNEQQQVYTLARIENQIVDD
                     LRDRLNELKSGRDDDIQVETHLRYFENLKKTADENIRMLDDWPGTITLRQTIDELLDM
                     GIVKNKMPDTMREYVAAQKALEDASRTREATQGRFRTLLEAQLGSTHQQMQMFNQRME
                     QIVHVSGGLILVATALALLLAWVFNHYFIRSRLVKRFTLLNQAVVQIGLGGTETTIPV
                     YGNDELGRIAGLLRHTLGQLNVQKQQLEQEITDRKVIEADLRATQDELIQTAKLAVVG
                     QTMTTLAHEINQPLNALSMYLFTARRAIEQTQKEQASMMLGKAEGVISRIDAIIRSLR
                     QFTRRAELETSLHAVDLAQMFSAAWELLAMRHRSLQATLVLPQGTATVSGDEVRTQQV
                     LVNVLANALDVCGQGAVITVNWQMQGKTLNVFIGDNGPGWPEALLPSLLKPFTTSKEV
                     GLGIGLSICVSLMEQMKGELRLASTMTRNACVVLQFRLTDVEDAK"
     gene            5009..6256
                     /gene="pgtA"
                     /locus_tag="ACHKUC_003567"
     CDS             5009..6256
                     /gene="pgtA"
                     /locus_tag="ACHKUC_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PgtA"
                     /protein_id="HFO6619762.1"
                     /translation="MLSSEYSILLIDDDADVLDAYTQLLEQSGYRVFACNNPFEAQAW
                     IQPDWPGIVLSDVCMPGCSGIDLMMLFHQDDQQLPILLITGHGDVPMAVDAVKKGAWD
                     FLQKPVDPGKLLSLVEEALRQRQSIIARRQYCQQTLQVELIGRSEWINQYRRRLQQLS
                     ETDIAVWLYGAPGTGRMTGARYLHQFGRNAQGEFVYRELTPDNAPQLNDFIALAQGGT
                     LVLSHPEHLTREQQYHLVQLQSQEHRPFRLIGIGDTSLVELAASNHIIAELYYCFAMT
                     QIACLPLTQRPDDIEPLFRHYLCKACQRLNHPVPEVGKEMLKEMMRRMWPNNVRELAN
                     AAELFTVGVLPLAETANPLMHVGTPTPLDRRVEDAERQIITEALNIHQGRINEVAEYL
                     QIPRKKLYLRMKKYGLSKEHYKI"
     gene            6503..6616
                     /locus_tag="ACHKUC_003568"
                     /pseudo
     CDS             6503..6616
                     /locus_tag="ACHKUC_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(6849..7172)
                     /locus_tag="ACHKUC_003569"
     CDS             complement(6849..7172)
                     /locus_tag="ACHKUC_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619763.1"
                     /translation="MSNRYWMISFHKNTRNLTVRSVPKKDIAHNIKLIIRPLNISIEK
                     FLSIEYTNVEGIPLTSRRITIMGLKPGPKPIAKSVGKPDQRRRDNKDTPGNTPGLKPS
                     KSTGK"
     gene            complement(7237..7881)
                     /locus_tag="ACHKUC_003570"
     CDS             complement(7237..7881)
                     /locus_tag="ACHKUC_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619764.1"
                     /translation="MMKKDMADSVSMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPSFRSEKEAESWLNELSFA
                     VIAPLYRVVPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQRQRITKILNESDTRRVAEEIKFRIQYLQDEEQKRVLSDLVNVLISKR"
     gene            complement(7902..8171)
                     /locus_tag="ACHKUC_003571"
     CDS             complement(7902..8171)
                     /locus_tag="ACHKUC_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6619765.1"
                     /translation="MVAQKLEAAGCWRRASARWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIASPSGEIFRKGTPVC"
     gene            complement(8250..8895)
                     /locus_tag="ACHKUC_003572"
                     /pseudo
     CDS             complement(8250..8895)
                     /locus_tag="ACHKUC_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(8880..>10078)
                     /locus_tag="ACHKUC_003573"
     CDS             complement(8880..>10078)
                     /locus_tag="ACHKUC_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6619766.1"
                     /translation="GENVLRAAIARIEWIFETFPSVCLSFSGGKDSTVLFHLLADVAR
                     RKKRRFSVLFIDWEAQYQCTIEHIQKMREMYHDVTETFYWVALPLTTVNGVSQFQPEW
                     ICWEPGVTWVRQPPEEAITDMTYFPFYRYAMTFEEFVPAFSSWFADNKCGVAVLTGVR
                     ADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKTRDIWIYHTRTRAI
                     YNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWARMCERVSGAASGAL
                     YANESGAYFALRKRISKPAHHTWRSYAMFLLDMMPERTAEHYHNKIAVYLRWYQTRGF
                     PDDIPDEQENDLGCRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKERR
                     NEWGIL"
BASE COUNT         2688 a         2490 c         2312 g         2588 t
ORIGIN      
        1 actccccagg gaaacgaccc tggggaaggt aattagtgct cttttatata tgcggctctg
       61 tgacgttcaa gttcaattgc accacgatgt gataaccagc aaaaaatgat gcaacaaata
      121 ataccgcagc caagaagata aaatccgcca tgccagccaa tatgatcgac cataatacca
      181 aacaggctgg tgcccagaga tgcaccgaag atatagctca taaaaccacg taaacctacc
      241 gcggagccaa cagcaaagct gggcacgatc tccatagtct gaacggaagc cagaaattgt
      301 ggaacgtaaa tcagacaacc aacaatggca gcaaaaattg tcaccataaa caacgattca
      361 cttttccagt agccaatcag gcaaatgaaa atcagcgcca tacaaatcat cgccaatggc
      421 atacgacgcc ctttaaacag tttgtctgac aaccaaccgg caagtagcgt ggaagggatc
      481 gcggcccatt caaaaaataa aaatgcgacg ctcatttgtt cttttgaaaa atgtttcacc
      541 gtcagcagat aaataggcaa ccagctaatc atcccgaagc gcaccatgta tacaaatacg
      601 tcaaccagtg agacatacca ggcattttta ttgcgtaata cataagtgca gaaaatctga
      661 aaggcgctca tgttttctgg tgcttttacc gtctgtcggg tattcaggac gactttttct
      721 tccggcatca tctcttccag agagggtaga ccttcctgat gtggggaacc tttaccgaga
      781 atcagcacaa ttaccgcaaa aacgatggcc acgcaggccg gaacgatata actcgcactt
      841 tgccagtgct cgctgccgag tagggcaaaa gcggcaccaa caatcggtgc aacaatacca
      901 ccaccgatgt tatgagagat attccagaaa gcaccaaccc gaccacgctc ccggcgaggg
      961 aaccagttag caatagtgat gaaagaagga ccaacgccca ttccctggaa aagaccattc
     1021 agaataacca atgccgcaaa aatccagaat gcggtgctga atcccaggcc aacgttaacg
     1081 atggcacata acaccagccc acacgccata aagactttcg gactggcttt atcggcaagg
     1141 ctactcatca ctcctttgct gataccatag gcgataagca tacagctact cagtacgcca
     1201 atttgtgtgg cgctgagatc taattgctct ttaagataag gcgttgataa ggtgaaatta
     1261 ttacgcacga tatagtatgc aagatacccc agaaatacgc taagtaacgc ctgcatacga
     1321 tagcgacgat atgtcacctg aattttctcc gccggaacac tatccgccgc ttgccccgtt
     1381 ttaaatattg ataacatgtc ttcacctgta ttttatattg atatgaacat caggagtatt
     1441 ctgacaatga gagttatata attgagatga aatatcaaac tgaaaaaatg tgtcacaatt
     1501 gacacaaaat aataaaatca aagcctgttt ttgacccatt ttattgatgc atagacaggg
     1561 cagatttatg ctttatctgg tttgttgtta gcatgtgcgg atattaacaa atctcatgct
     1621 gattatcggg taatctctga ttaatatatt aacggtctct ttgaacagac gtgttatcag
     1681 acgaagtgtc tgaaacgggt aatttttgtc tcaatgcgat ttttgcggtg tattaatatt
     1741 tatgacctgg atcacaaacc gattttttag ttggtgcgaa atcggcatca aaaccatgat
     1801 tcttttactg ttgttgatgg atgtcggggc cgtcagggct caacgaaatg aactggtgat
     1861 ggccaccaca ttctcacccg gagctatcgc gtggataata cagcgctggc aaacagagcc
     1921 tgagtcggta atgatccgta cgcttaaccg caccagtgcc tcactggaac agttgcttga
     1981 tacggtcaac gtagaaaacg tcgatctgat cctgacttca tcaccaatgc tgctccagca
     2041 ccttcaggag caccagaaac tggccccgtt tgatgatgca cccgcagaaa gccaaaacct
     2101 ggtgccggag tcgatccgtg caacctccgt tgcagtagca atatcaggtt ttggtctgct
     2161 tattaatcgt cctgcgcttt ctgtaaaaca ccttcctgcc cctgctgact gggacgatct
     2221 tgctttgccg atctatcagg acgctttatt gatgagtagt ccgtcgcgtt cagatactaa
     2281 ccatttaatg gttgagtcat tactacagca aaaaggctgg gtgaaaggat gggaaacgtt
     2341 gctgacaagt gcaggaaacc tggtgacaat ctcctcccgc agttttggcg tagccgataa
     2401 aataaaaagc ggactcggcg ttgccggccc cgttatcgat aactatgcta atttactgtt
     2461 aaatgacccc catatctctt ttacctattt cccccggtct gcggtatccc ccacctacgt
     2521 tgcaattctc aggaaaagcc cacacgccga tgcagcccgt cgctttattc actacctgtt
     2581 aagtccgaaa ggacagcgta ttctggccga tgccaataca ggaaaatatc cggtgacacc
     2641 acttgccgca gacaatccgc gggcaacgca acaacagctc ctgatgaatc aacccccgct
     2701 gaattatcac cttatcctga aacgtcagcg tctggtgcag cgcttgtttg atacggcaat
     2761 tagctttcgg ttcgcacagc taaaagatgc ctggcgagcg ctgcacagta ccgaggcacg
     2821 cctgaagaaa ccattaccag aaatacgtgc actgttaaca caaatgccag ttgcagcggc
     2881 cagtagtgaa gatcccgtct ggctggcaca gttcgataac aaaagctttg ccgagcagca
     2941 aatgatggaa tggcaactct ggtttctgaa taaccagcgc ctggcgataa aaaaactgga
     3001 agagctgaaa tgaaaaacat aacgctgtgg cagcgtttaa gacaggtcag tatcagtacc
     3061 agcttacgtt gcgcatttct gatgggggca cttctgaccc tgattgtcag tagtgtcagt
     3121 ctgtattcat ggcatgaaca aagctcacaa attcgttact cgctggataa gtattttccc
     3181 cgtattcact ctgctttcct tattgaaggg aacctgaatc tggtggtaga ccagctaaat
     3241 gaatttttgc aggctcccaa caccacggtg cgattgcaac ttcgtaccca gattattcag
     3301 catctcgaca ccatagaacg gcttagtagg ggactgtcat cccgggaacg ccaacaactg
     3361 acggtcattt tgcaggacag tcgatcactg ttatccgagt tggatcgtgc gctttacaac
     3421 atgtttttac tacgggaaaa ggtgagtgag ctatcagcgc ggattgactg gttacacgat
     3481 gattttacta ccgagcttaa ttctttagtg caggatttca cctggcagca gggaacgctg
     3541 ctggatcaaa tcgcctcccg acagggcgat acggcgcaat acctgaagcg atctcgtgaa
     3601 gtgcaaaatg aacagcagca ggtttatacc ctggcacgca ttgaaaatca gattgttgac
     3661 gatctgcgtg acagactcaa tgagctcaaa tcaggacgtg atgacgacat acaggtggaa
     3721 actcatctcc gttattttga aaatctgaaa aaaacggcag atgaaaatat acgtatgctg
     3781 gatgactggc ctggcaccat taccctgagg cagaccatcg atgaattgct ggatatggga
     3841 atcgtaaaaa acaaaatgcc ggatacgatg cgtgaatatg tcgccgccca aaaagcctta
     3901 gaggatgcca gtcgcaccag ggaagcgaca cagggtcgct tcagaacgtt actggaagcg
     3961 cagcttggca gtactcatca acaaatgcag atgtttaatc aacgaatgga acaaattgtt
     4021 cacgttagcg gtgggctgat cctggtggcg acagcactgg cgttactgct tgcatgggta
     4081 ttcaaccatt attttatccg ctcacggttg gtgaaacgct ttaccctact gaatcaggcc
     4141 gttgtgcaaa ttggtctggg aggcacggaa acgactattc cagtttatgg gaatgatgaa
     4201 ctggggagaa ttgcaggatt attacgccat actctcggcc aactcaatgt gcaaaaacag
     4261 caacttgaac aagaaattac cgatcgtaag gtgatagaag ccgatctgcg tgccacccag
     4321 gacgaactga ttcagacagc aaagttggcg gtagtcgggc aaacgatgac cacgctggcc
     4381 cacgagatca atcagccgct aaatgcgctg tcaatgtatc tgtttacagc ccgcagggcc
     4441 attgaacaga cccagaaaga acaggccagc atgatgcttg gtaaagccga aggggtgatt
     4501 agtcgtattg acgccattat ccgttcacta cggcagttta cccggcgcgc cgaactggaa
     4561 acatcactcc atgccgttga tttagcacag atgttcagtg cggcctggga acttctggcc
     4621 atgcgtcatc gctctctgca agctacgctt gttctgccgc aaggtacagc cacagtttca
     4681 ggtgatgagg tcagaaccca gcaggtactg gttaacgtac tggcgaatgc gcttgatgtt
     4741 tgtgggcaag gcgctgtcat taccgttaac tggcaaatgc agggtaaaac gctgaacgta
     4801 ttcattggcg ataatggccc gggctggcct gaggcattgt tgccttcgtt attgaagccg
     4861 tttaccacca gtaaagaagt aggactgggt attggtcttt caatttgtgt gtcgttgatg
     4921 gagcaaatga aaggggaatt gcggctggca tcaacgatga ccaggaatgc ctgtgtggta
     4981 ctgcaattca gactaacgga tgtggaagat gctaagtagt gaatattcga ttctgctgat
     5041 tgatgatgat gctgacgtgc tggacgccta cacacagtta cttgaacagt cgggctatcg
     5101 tgtttttgcc tgtaacaatc cttttgaggc gcaggcgtgg atacaaccag actggccagg
     5161 cattgtgcta agtgatgtgt gtatgcctgg ctgctctggt atcgatttga tgatgttatt
     5221 ccaccaggat gatcaacagc tccccattct gcttatcacc gggcacggcg atgtgccaat
     5281 ggcagttgat gcggtgaaaa aaggcgcgtg ggattttttg caaaaaccgg tcgatcccgg
     5341 caaattgctt tctctggttg aagaggcact acgccagcgt caatcaatca ttgcgcgtcg
     5401 tcagtattgc caacagacat tgcaggtgga gttgataggg agaagtgaat ggataaatca
     5461 gtatcggcga cgtttacagc agttgtctga gacagatatt gccgtctggc tctacggtgc
     5521 ccctggcaca ggacggatga ccggtgcacg ttatctgcac caatttggac gtaatgcaca
     5581 aggcgaattt gtctaccgcg agctgacacc agacaatgca ccacagctta atgatttcat
     5641 tgcgctggct caggggggaa cattagtgct cagccatcct gaacacctga cgcgtgaaca
     5701 gcagtaccat ctggttcagt tacaaagcca ggaacacaga ccttttcgcc tgattggtat
     5761 tggagacacc tcgctggtcg agctggccgc cagcaaccat atcatcgcgg agctctatta
     5821 ctgctttgca atgacacaaa tcgcttgcct gccattaact caacgccccg acgatattga
     5881 accactgttt cgccattacc tttgtaaagc atgtcaacgg cttaaccatc ctgttccgga
     5941 agtgggaaaa gagatgctca aagagatgat gcgccgcatg tggccaaata atgtgcgaga
     6001 gctggccaat gcagcagaac tatttactgt aggagtatta ccgctggcag aaacagcaaa
     6061 tccgttgatg cacgtcggaa ccccgacacc gcttgacaga cgagtggagg acgctgagcg
     6121 acagataatc acagaggcgc ttaatatcca tcaggggcgt atcaatgagg ttgctgaata
     6181 tttacaaatc ccacggaaaa aactctatct cagaatgaaa aaatacggtc ttagtaagga
     6241 gcattataaa atttaaccat cagacttgtg cgcttgttca taatgttatg tcattgcctg
     6301 atgttgataa aaagacggac gctcctcctg taagacctgt ctcttataca caagtcccca
     6361 cccggggatt tgtgatgtct gtcagtctgc tttagggggg gctctttcca tccggtactt
     6421 tccttcctcc ccggtagcca ttttttgaac tttttcttca cccgttgatc ttctcttata
     6481 tttcatttta ttcgactctg ttatggtact ttctgattgt tattcatggg ctaacgagca
     6541 gtttggacat gcccgtctcg atgatccgcg tagtacacga cgccttgtat cactggcctc
     6601 ttctctcgct catgccaggt cgtgatcgat tcagatgcca ttttgcacct tcagaatttg
     6661 agcttgctgc agttgttcct gataaggatc tggataaata tgacgagtat ttaccagagt
     6721 ctttgcttgc gaaagggtac ggggcaaagg cgtatgatat agaaagaacg agcatctggt
     6781 tgctgaaaca ccttcagtgg actggatata tagtcagtgt ataaatgctt gtttataccc
     6841 ttattttttt acttacctgt ggacttactt ggctttagtc ccggagtatt gccaggagta
     6901 tctttattat cgcgtcggcg ttgatccggt tttcctaccg acttagcaat tggttttgga
     6961 cctggtttaa gacccatgat agttatcctt cgagatgtaa gaggaatgcc ctcaacattg
     7021 gtatattcta tactgagaaa tttttcaatg gaaatgttca acggccgaat gattaacttg
     7081 atattgtgag caatatcttt ttttggcaca gagcgaaccg ttagattgcg cgtattttta
     7141 tggaaggaga tcatccaata acggttactc accgatcttc ttcatggtaa tacgcacctg
     7201 ttttattaac atatagaaaa attactccgg catgttttat ctttttgaaa tcaggacatt
     7261 taccagatca cttaaaactc tcttttgttc ttcatcctga agatattgaa tccggaattt
     7321 aatctcttct gccacccgtc ttgtatcgga ctcattaagt atttttgtta ttcgctgtct
     7381 ctgatgttca ggctggtctt ccatatttcg taaaaccaga tgaaataata tatcataaag
     7441 tgcgcaatgc catgaataac catcattggt taaaataagc tcccaaaccc gctcctgctc
     7501 ggcaataagc tgcagaacaa aggactcaac ctgttctgtt tgttcgggga caacacggta
     7561 tagcggagca atcacagcaa aagacagttc atttaaccag gattccgctt ccttctcgct
     7621 cctgaacgat ggacagtttt gtattcttcg ttcgtattca cgggtaatct gctccagaat
     7681 atcctccggc ggtgtcagcc acttattcct gagcaataca ctggttgatg gatgacagct
     7741 ggctatttca gcaataattt cacgtaatgt ctccgggctg aacttcttca gctgattgta
     7801 gtgctcccgg gtaaaaccat gttcatcgca taaaagttgc catgtcattg acacactgtc
     7861 cgccatatct ttcttcatca ttaatacctg ttacctgaat atcaacagac cggtgtacct
     7921 tttctgaata tctccccgga cgggctggca atccccattc gcctcagcgt ggcatctgca
     7981 gctttcgcca cttcgctgat atccagcttt tcaggtagcg gcggggacga tatctgcgcc
     8041 agacaataat tccggcgcaa caacaaccac tcccgttgtg cttctgtaca ttcaacattt
     8101 cccataacaa aaagccagcg ggcagaagct ctgcgccagc aacccgccgc ctccagcttc
     8161 tgtgcaacca tgtcttccac gacttccggg gtactatttt tcctgctcaa aacatcaact
     8221 cactgaaata atactcatat atgacggtct cagcgaaccg tccatgccgg gctgaaatcc
     8281 tcttcctgaa acaactccgt cagtccgcta atctgtttca gcctcagcac ttcatcctgg
     8341 tccatcccca ggctctgtac caatgcgttc atccgtccag cccaggcgtg acaggtccct
     8401 gacaatatca gacattgagg ttatctggtg tttacccctg gcccggttat gccgtatggt
     8461 ggccgccata cgtgtggcct gtccctttct ttccggatta atacaggcca ctggcaacca
     8521 gcctttcagg cgttttcctg tacctgcttc cctgcccagt aactgacgat gaaaaccatc
     8581 caccaccaga taggggttct tctccgccga caccaccacg ggttgggtga aaccatcttt
     8641 ctccagtgac tgcttcagca gtttcttctc acccggcgcc atgacattgg ggttataatc
     8701 gttggccacc acctcatctg ctttcaccca cagcacacag tccacaggct cctgtgcaaa
     8761 agggctgact tcatgaagta ccctgcgcag atgattcaga gcctgtatag tctctttttc
     8821 tgatcgttcc tggctgaagt actgacgaat catgtcactc agtacctcca cttcaggtgt
     8881 cacagaatcc cccattcatt cctcctttcc ttcatacgct gcaggtaacg ttcatagtgc
     8941 cggggtttgt tcgggctgaa ggacagcgtc cggcaccaga aatcattctt tataagagtc
     9001 ttacagatac gtcgccagga cgggatatcc cggcagccca ggtcattctc ctgttcatcg
     9061 ggaatgtcat ccgggaagcc ccgcgtctga taccagcgca gatagacagc tatcttgttg
     9121 tggtaatgtt ctgctgttct ttccggcatc atatccagca gaaacatcgc gtagctgcgc
     9181 caggtatgat gggctggttt tgatatgcgc ttacgcaggg caaaataagc accgctttca
     9241 ttggcataaa gtgccccgct ggcagcccct gatacccgct cacacatcct ggcccaggta
     9301 tccggctcca gaacatggta aagccacagt cccttacgtt gttcaggacc aaaaggttca
     9361 cagacacgca tgttgcgtaa cggtacgccg gcacggtaca tcaggtcata caggggattg
     9421 tagatggcac gggttctggt gtgatatatc cagatatcac gagttttcca gtcatacagt
     9481 ggatacatgg tgtagtaaaa cccttcaggc gatgccgtgg tccagggctt atcatcggcg
     9541 tatctcagtt tgcgctggga caccagtccc atgaaccggt taagggactc atccgcacgg
     9601 acaccggtca gtactgccac accacattta ttgtctgcaa accaggaaga aaatgccgga
     9661 acaaattctt caaacgtcat ggcataccga taaaatggga aatacgtcat gtctgtaatg
     9721 gcctcttccg gtggctggcg aacccaggtc actcctggct cccagcatat ccactccggc
     9781 tgaaactgcg agacgccgtt taccgtggtc aggggaagtg ccacccagta aaaggtttcc
     9841 gtcacatcat ggtacatttc ccgcatcttc tgaatatgtt caatggtgca ctggtactga
     9901 gcctcccagt caataaacag tacagagaaa cgacgttttt tcctgcgggc cacatcagcc
     9961 aaaagatgga aaagcacggt ggaatcttta cccccggaaa aagagagaca aactgatgga
    10021 aatgtttcaa aaatccactc aatacgagca attgctgcac gcagaacatt ctctccag
//
LOCUS       DAZFRG010000178        10035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFRG010000178 DAZFRG010000000
VERSION     DAZFRG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.178"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..719
                     /locus_tag="ACHKUC_003574"
     CDS             60..719
                     /locus_tag="ACHKUC_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO6619767.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(796..1776)
                     /gene="ygfZ"
                     /locus_tag="ACHKUC_003575"
     CDS             complement(796..1776)
                     /gene="ygfZ"
                     /locus_tag="ACHKUC_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO6619768.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            2019..2285
                     /gene="sdhE"
                     /locus_tag="ACHKUC_003576"
     CDS             2019..2285
                     /gene="sdhE"
                     /locus_tag="ACHKUC_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO6619769.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            2266..2673
                     /locus_tag="ACHKUC_003577"
     CDS             2266..2673
                     /locus_tag="ACHKUC_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO6619770.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2713..3234)
                     /gene="fldB"
                     /locus_tag="ACHKUC_003578"
     CDS             complement(2713..3234)
                     /gene="fldB"
                     /locus_tag="ACHKUC_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO6619771.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            3346..4242
                     /gene="xerD"
                     /locus_tag="ACHKUC_003579"
     CDS             3346..4242
                     /gene="xerD"
                     /locus_tag="ACHKUC_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO6619772.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            4267..4977
                     /gene="dsbC"
                     /locus_tag="ACHKUC_003580"
     CDS             4267..4977
                     /gene="dsbC"
                     /locus_tag="ACHKUC_003580"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO6619773.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            4983..6716
                     /gene="recJ"
                     /locus_tag="ACHKUC_003581"
     CDS             4983..6716
                     /gene="recJ"
                     /locus_tag="ACHKUC_003581"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO6619774.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSAAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6807..7905
                     /gene="prfB"
                     /locus_tag="ACHKUC_003582"
     CDS             join(6807..6881,6883..7905)
                     /gene="prfB"
                     /locus_tag="ACHKUC_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO6619775.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7915..9432
                     /gene="lysS"
                     /locus_tag="ACHKUC_003583"
     CDS             7915..9432
                     /gene="lysS"
                     /locus_tag="ACHKUC_003583"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6619776.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(9475..10023)
                     /gene="idi"
                     /locus_tag="ACHKUC_003584"
     CDS             complement(9475..10023)
                     /gene="idi"
                     /locus_tag="ACHKUC_003584"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO6619777.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
BASE COUNT         2372 a         2502 c         2752 g         2409 t
ORIGIN      
        1 ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttttccg gagtaagtta
       61 tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt gccaacagcg
      121 tcagtcacgg cattgggctg gtgtttggta tcgttgggtt ggtgttgcta ctggttcagg
      181 cggtggatct caatgccagc gccacggcga taaccagtta cagcctctat ggcggcagta
      241 tgatcctgct gttcctcgct tcgacgctct atcacgccat tccccatcaa cgggcaaaaa
      301 tgtggctgaa gaaatttgac cactgtgcca tttatctgtt gattgccgga acctacacgc
      361 cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt gttatctgga
      421 gcctggcatt gctgggtatt ctgtttaaac tgaccatcgc gcaccgattc aaaattttat
      481 ctctggtgac ctatctggcg atgggctggc tgtcgctggt ggtaatttat gaaatggcag
      541 ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggtgtggtt tattcgctcg
      601 gggtgatttt ctacgtctgc aagcgcattc catacaatca tgccatctgg cacggctttg
      661 tgctcggcgg tagtgtgtgc cactttctgg cgatctattt gtatattggg caggcgtaat
      721 tggcgtgcct ttgtaggcct gaaaagacac accagtgtcg catcaggcga taaatgcctg
      781 atgcgataca gagatttact cttccagcga atatggcagt ggctcgatac gcaatgtatt
      841 cgcatcgtca cgcacgcgga agatgctgtc cggttccata tcgttattca tgacaacctg
      901 caccacaacc tgaccatctt ccagtttgac cgcagccagc accgtaccgg tacgacgcca
      961 gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc tggcgctacc
     1021 cttcagcaac cagagcgcgc gtttgttggc accacggaat tttgctcgcg ccaccatctc
     1081 ttgtccggta tagcagcctt tcttaaagct gataccgccc agcgcctgga ggttggtcgc
     1141 ctgggggata aactgcccgc tgttggcggc atcaatcacc ggaaaacccg cttcaatgtt
     1201 taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt tatcggtcag
     1261 catattggca gtagcttcat cggttacgat caggaaacgt tctgccgggt gttcaaacca
     1321 tagcaacgtg gtcgcgcctt ctttgactac ctgtttttct ctcgaaggta gttcactaaa
     1381 gagatttgcc aacgcggcgc gcgcctgaaa accggcaaca ccaagcagca cacgctcgtc
     1441 gtctggcgcg atggtcactt tagagaacac cgcatatttt ttcagttcag tcagctgcgg
     1501 ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga acagacgtaa
     1561 attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct gatcttcagt
     1621 catctggctg acatctgctg tcacctggcc ttgcatatat ttttcgctgt ccgcgccagt
     1681 aatagtggca agcgcccagt catcaagcgt catcagcgtc agtggcaaac gggcagaagc
     1741 cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag ttgcttaacg
     1801 gcgtattaat aactaatggt aaaagagcta tgaggcaatg caagtgcttt cactacgcga
     1861 tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc cttgaattgt tgtgaattaa
     1921 tttcgcgatc gcgattgtgt ttactgatat tcccgtaaaa gaagtcgaaa aacacgttac
     1981 aatagccggg tactggattt tcgtgagaca ggaagaagat ggacattaac aacaaagccc
     2041 gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc atgccgtttt
     2101 tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt cgtctgctgg
     2161 aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg gccgatgcag
     2221 aactggaaat gatggtccga ctcattcaga cacggaaccg ggaacgtggt cctgtggcaa
     2281 tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca tgggctggtt
     2341 gccgctgtta ttttactcat gccctggccg ctcagttaca ccccgttatg gatggtgtta
     2401 ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gcattaatgc tcgccagggg
     2461 gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg gagcatcgtc
     2521 aaagcgccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga cagcggaaaa
     2581 cggcaacatt tatggctggc agctgacagc atggacgaag ccgaatggcg ggatttacgg
     2641 cggatattgt tacaacaaga gacgcaaaga taaagatgac ggcaaagcat atgtgctaag
     2701 ccgtcagtga catcaggcgt aatgctctgc catttcgttg aggatttgct cgcaccagct
     2761 ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca gacccacgaa
     2821 cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt ccgttggcca
     2881 gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac cgagcgcatc
     2941 gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa caattttacc
     3001 ttcgaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct ggatttcgcc
     3061 aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta atttcgggga
     3121 gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc ggattttttc
     3181 tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat tcataacgta
     3241 agcttctcga ttctgctgtg gcttttgcgg ctagtgtatc agaatcgctt tagtcacggc
     3301 acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa acaggatctg
     3361 gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc tgaaaatacg
     3421 ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca ccgcgggttg
     3481 acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg gctggagggc
     3541 gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt gttccagtat
     3601 ctttatcgcg aaaagtttcg tgaagatgat cccagtgcgc atctcgcttc accgaaattg
     3661 ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggtcg aacgtttatt acaggcacca
     3721 ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt gtatgctacc
     3781 ggactgcgtg tctctgaact ggtcgggctg acaatgagtg atatcagcct gcgtcagggc
     3841 gtggtgcggg tcattggtaa aggcaacaaa gagcgcctgg tgccgttagg tgaagaggcg
     3901 gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgttgaa tggtgtgtca
     3961 attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac cttctggcac
     4021 cgcattaaac attatgccgt gctggcgggt atcgacagcg aaaagctgtc accgcatgtg
     4081 ttgcgtcacg cttttgccac tcacttatta aatcatggtg cggatttacg cgtggtgcag
     4141 atgctactgg gccacagcga tctctccacc acgcaaattt atacgcatgt cgctaccgag
     4201 cgtctgcgac aacttcatca acagcatcac ccgcgggcgt gatgtctgaa aagaacagga
     4261 aaatttatga agaaaggttt tatgttattt accttgttgg cagcgttttc aggctttgtt
     4321 caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa aagcagcgat
     4381 attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg tgtgttgtac
     4441 atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag tggcacggct
     4501 ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga aaaagagatg
     4561 atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga tattacctgt
     4621 ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg gatcaccgtg
     4681 cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga aatgaaagct
     4741 atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg taaaagcgtc
     4801 gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt ccagcttggc
     4861 gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg ttaccagccg
     4921 ccgaaagaca tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg taaataattc
     4981 gcgtgaaaca acagatacaa cttcgtcgcc gagaagtcga tgaaacggcc gacttgcccg
     5041 ctgaattgcc tcccttgctg cgccgtttat atgccagccg gggtgtgcgt agcgcgcagg
     5101 aactggaacg cagtgttaaa ggcatgctgc cctggcagca actgagcggc gtcgaaaagg
     5161 ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg gtcggtgatt
     5221 tcgacgccga cggtgcgacc agcacggctc taagcgtgct ggcgatgcgc tcgcttggtt
     5281 gcagcaatat cgactacctg gttccaaacc gtttcgaaga cggttacggc ttaagtccgg
     5341 aagtagtcga tcaggctcat gcccgtggcg cgcagttaat tgtcacggtg gataacggta
     5401 tttcctccca tgcgggggtt gaacacgctc gttcgttggg cattccggtt attgttaccg
     5461 atcaccattt gccaggcgac acattacccg cagcggaagc gatcattaac cctaacttgc
     5521 gcgactgtaa tttcccgtcg aaatcactgg cgggcgttgg cgtggcgttt tatctgatgc
     5581 tggcgttacg cacttttttg cgcgatcagg gctggtttga tgaacgcggc atcgcaattc
     5641 ctaatctggc agaactgctg gatctggtag cgttggggac agtggcggac gtcgtgccgc
     5701 tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt gccggaaagt
     5761 gccgtccggg gattaaagcg ctgctggaag tggccaatcg cgatccacaa aaactcgccg
     5821 ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccgggcga ctggacgata
     5881 tgtccgttgg cgtggcgctg ttgctgtgcg acaacatcgg cgaagcgcgc gtgctggcaa
     5941 atgaactcga tgcgctaaac cagacgcgaa aagagatcga acagggaatg caagttgaag
     6001 ccctgacctt gtgcgagaaa ctggagcgca gccgcgacac gctacccggc gggctggcaa
     6061 tgtatcaccc cgaatggcat cagggcgttg ttggcattct ggcatcgcgt atcaaagagc
     6121 gttttcaccg tccggttatc gcctttgcgc cagctggtga tggtacgctg aaaggttccg
     6181 gtcgctccat tcaggggctg catatgcgtg atgcgctgga gcggctagac acgctttacc
     6241 ccggcatgat gctcaagttt ggcggccatg cgatggcggc aggattgtcg ctggaagagg
     6301 ataaattcga actctttcag caacgctttg gcgaactggt taccgagtgg ttagatcctt
     6361 ctttattgca aggggaagtg gtatcagacg gcccattaag tgcggcagag atgaccatgg
     6421 aagtggcgca gctgttgcgt gatgctggcc cgtgggggca gatgttcccg gaaccgttgt
     6481 ttgacggtca tttccgtctg ttgcaacagc ggctggtggg cgagcgtcat ttgaaggtga
     6541 tggtcgaacc ggtcggcggc ggtccactgc tggacgggat tgcttttaat gtcgataccg
     6601 ccctctggcc ggataacggt gtgcgcgaag tgcaactggc ttacaagctc gatatcaacg
     6661 agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca atttagcgtc
     6721 atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca ccgcattttg
     6781 actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat aatcgcattc
     6841 aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga cgccaagaaa
     6901 gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa cgaacccgaa
     6961 cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga caccctcgac
     7021 caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt agaagctgac
     7081 gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga aaaactggcg
     7141 cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg ctacctcgat
     7201 attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct tgagcgtatg
     7261 tatctgcgct gggctgaatc gcgtggtttc aaaactgaaa tcatcgaaga gtcggaaggt
     7321 gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc ttatggctgg
     7381 ctgcgtacag aaaccggcgt tcaccgcctg gtgcgtaaga gcccgtttga ctccggcggt
     7441 cgtcgccaca cgtcgttcag ctccgcgttt gtttacccgg aagttgatga tgatatcgat
     7501 atcgaaatca atccggcgga tctgcgcatc gacgtttatc gcgcatccgg cgcgggcggt
     7561 cagcacgtta accgtaccga atctgcggtg cgtattactc acatcccgac cgggatcgtg
     7621 actcagtgcc agaacgaccg ttcccagcac aagaacaaag accaggccat gaagcagatg
     7681 aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca ggcgatggaa
     7741 gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct tgatgactcc
     7801 cgcattaaag atctgcgtac cggggtagaa acccgcaaca cgcaggccgt gctggacggc
     7861 agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac caacatgtct
     7921 gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact gaaaacgcgt
     7981 cgtgagaagc tggcgaacct gcgtgagcag gggattgcct tcccgaacga tttccgtcgc
     8041 gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga agaactggaa
     8101 gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgtcgtat tatgggtaaa
     8161 gcgtctttcg ttaccctgca ggacgttggc ggtcgcattc agttgtacgt tgcccgtgac
     8221 gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg cgatatcctc
     8281 ggcgcgaaag gtaaactgtt caaaaccaaa accggtgaac tgtctatcca ctgtaccgag
     8341 ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg cttgcaggat
     8401 caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga atcccgcaac
     8461 acctttaaag tgcgctcgca gatcctctct ggtattcgcc agttcatggt gaatcgcggc
     8521 tttatggaag ttgaaacgcc gatgatgcag gtgatccctg gcggtgccgc tgcgcgtccg
     8581 tttatcactc accataacgc gctggatctc gacatgtacc tgcgtatcgc gccggaactg
     8641 tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa ccgtaacttc
     8701 cgtaacgaag gtatttccgt acgtcataac ccagagttca ccatgatgga actctatatg
     8761 gcttacgcag attacaaaga tcttatcgag ctgaccgaat cgctgttccg tactctggca
     8821 caggatattc tcggtaagac ggaagtgacc tacggcgacg taacgctgga cttcggtaaa
     8881 ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga aactgacatg
     8941 gcggatctgg acaacttcga ctctgcgaaa gcgattgctg aatctatcgg catccacgtt
     9001 gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt ggcagaagca
     9061 catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc gctggcgcgt
     9121 cgtaacgacg ttaacccgga aatcacagac cgctttgagt tcttcattgg tggtcgtgaa
     9181 atcggtaacg gctttagcga actgaatgac gcggaagatc aggcgcagcg cttcctggat
     9241 caggttgccg cgaaagatgc aggtgacgac gaagcgatgt tctacgatga agattacgtc
     9301 accgcactgg aacatggctt accgccgaca gcaggtctgg gaattggtat cgaccgtatg
     9361 gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc ggcgatgcgt
     9421 ccggtgaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg ttttttaatt
     9481 gtgctgcgcg aaagcagaca acaattttct tgcttcacta ttggctgcct gcatcaccat
     9541 ccacggactg aacgcccacg gcgtggcatc aataccgtgt aatacatctg ctaaatcaca
     9601 ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg tgcgtgcggc
     9661 aaataccgga cacacttcat tttccacaat gccattcgga tcggtggcgc gatagtagaa
     9721 gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac ggcaacggcg
     9781 gatcaccgcg tcttcgttgc tttctcccag ttgtgggtgc ccacaaaccg agttagtcca
     9841 cacgccaggc cacgcttttt tgctaagggc gcggcgggta actaataatt gccctttggc
     9901 attaaacagc caactggaga acgcgagatg taagagagtg tctgccgtgt gtgcggcata
     9961 cttttccagc gtacccgtgg gaactccctg tgcattcaat aaaatgacgt gttccgtttg
    10021 cataatttct cacat
//
LOCUS       DAZFRG010000179         9961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFRG010000179 DAZFRG010000000
VERSION     DAZFRG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.179"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..1281)
                     /locus_tag="ACHKUC_003585"
     CDS             complement(121..1281)
                     /locus_tag="ACHKUC_003585"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6619778.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIAAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPVGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1287..1958)
                     /locus_tag="ACHKUC_003586"
     CDS             complement(1287..1958)
                     /locus_tag="ACHKUC_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO6619779.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2106..3587)
                     /gene="tolC"
                     /locus_tag="ACHKUC_003587"
     CDS             complement(2106..3587)
                     /gene="tolC"
                     /locus_tag="ACHKUC_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO6619780.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3792..4421
                     /gene="nudF"
                     /locus_tag="ACHKUC_003588"
     CDS             3792..4421
                     /gene="nudF"
                     /locus_tag="ACHKUC_003588"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO6619781.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4422..4844
                     /locus_tag="ACHKUC_003589"
     CDS             4422..4844
                     /locus_tag="ACHKUC_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO6619782.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4869..5696
                     /gene="cpdA"
                     /locus_tag="ACHKUC_003590"
     CDS             4869..5696
                     /gene="cpdA"
                     /locus_tag="ACHKUC_003590"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO6619783.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            5696..6277
                     /gene="yqiA"
                     /locus_tag="ACHKUC_003591"
     CDS             5696..6277
                     /gene="yqiA"
                     /locus_tag="ACHKUC_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO6619784.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6306..8198
                     /gene="parE"
                     /locus_tag="ACHKUC_003592"
     CDS             6306..8198
                     /gene="parE"
                     /locus_tag="ACHKUC_003592"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO6619785.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8243..9112)
                     /locus_tag="ACHKUC_003593"
     CDS             complement(8243..9112)
                     /locus_tag="ACHKUC_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFO6619786.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGGFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9126..9860)
                     /locus_tag="ACHKUC_003594"
     CDS             complement(9126..9860)
                     /locus_tag="ACHKUC_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO6619787.1"
                     /translation="MNSYLKISLAGITMLSCLSPTLVCAEQPHGFIDYRHEYLDDTRT
                     HADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKK
                     FWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRN
                     NRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWHDGHKNYTASFKVGYKMGSWIPYMS
                     IADYKGVDKTSSNRQIRWRWGLTYTF"
BASE COUNT         2389 a         2406 c         2582 g         2584 t
ORIGIN      
        1 attaaattga gtattaatgt taaataaagg ttggatatgt atgtgtttat atattgatga
       61 gaattatgaa tggtggatta taagagattg ccccgcttat cggggcaata ttaagctgta
      121 ttactcaaca aaaatatgcg gataaaaccg agacatatcc tgggtgatca atgcacggtc
      181 ttcacgaatg ccaattccga caggttgatc gttcaccagc cagctaccaa tcagcatata
      241 gctgtcgccg aatttcggta acgggtggaa ttgctgaaca atcatccctt cttcgccata
      301 cggaccttcc gctgctgcaa tggttttgcc gttctcaatg atcgacacgt ttgcgccttc
      361 acgggagaag atcggtttaa ccacatattt ttccatttgc ggatgatcat cttccgcaaa
      421 ataagcgggc agcaggttcg ggtgattcgg gaacatctcc cacagtagcg gtagaagcgc
      481 tttgttggag ataatgctct tccacgccgg ttccagccag cgtacgcctg catcctccag
      541 cttggtggag aacatctcac gcaacataaa ttcccacgga tacagcttga acaggttgga
      601 aatcacctga tcctgtaaat ccgtgaactg acctttttca cctaacccga tatcgtcgat
      661 gtagaggaac tcggtagcga tttcagcttc cgtcgcgcag tcctgcaaat actgaatggt
      721 tccgcgatct tccaccgtgt cgcgacagca ggtgagatgc agcaactgga agccatactg
      781 ttcacgcagt tcaacgaagc gatcgatcag cttttcttgc aaactgttaa actggtcgct
      841 gccctccggc aagttaccgg cgttaagctg atcttccagc cagatccact gaaagaacgc
      901 cgcctcgtat agtgacgttg gcgtatcggc gttattttcc agaagtttag gttcaccagt
      961 gccatcccat gccagatcaa ggcgcgaata aagcgatggc tggtgcgtca gccatgactg
     1021 gcgcacaaaa ctccaggtgt gttttggtat gcggaatttg gtcatcagct catcgctggc
     1081 gatcactttt tccaccactt tcaggcacat ctggtgcagt tcggcggtga cttcttccag
     1141 cttttcaacc tgagagaggg tcaacttgta gtaagcatct tcacaccagt acggctcgcc
     1201 gtacatggtg tgaaaattga aaccgtattc gtgggctttt tcgcgccagt ccgggcgctc
     1261 ggtaatactg actctttcca tcggtatcag ccacccattg aacgagaaga ggtgccggtt
     1321 gcgctacgct gcatagtgct ttgtttggca acagattcac caaaaccgcc acgggtaaca
     1381 gtagttgtgg tcgccggttt tggtgccatt gccgtcttcg gtacggtcat ggtgcggcct
     1441 ggctgggctg cgccatagtt tttaccggtc gcgtcggtat atttaccgta agccgggctg
     1501 gccgggtttt tcgaggagaa cagcggctgc tgtgcaaatc ccgcgccgcc gcccatcaga
     1561 cgccccatca tgtaaccggc catcagcggc atccagaaac tcccgctgga ttgctgggcc
     1621 tgcgcctggt tttctggtgc catgccagcc tgggctggtg cctgctggca ctgaccttca
     1681 ccaaattcag caacacagtc ttcacgggtg gcgtatttcg gcgcagtacg ttcggcttct
     1741 ttcagcgcat tgttgtacgc ggtggtacat tcggcgcttt tgcctgggtt tgcagctgaa
     1801 cagtcgtcag cattttgata gagagacact gtttcatcac tcttttcaca gccagccagc
     1861 ataaaaacag tggcaaccgc gagagcgact ggtgtcagat ggcgtgcgct ccagtttttg
     1921 cggaacgatg cgtggcgtat ggattttgtc cgtttcattt ttgtcttccg ggaccagtgg
     1981 taaataccca tcagaataga ggatggctgg tcgaaattga agcgagaagg gggaagaatg
     2041 cggcagataa cccgtatctt tacgttgcct tacgttcaga cggggccgaa gccccgtcgt
     2101 cgtcatcagt tacggaaagg gttatgaccg ttactggtgg tagtgcgtgc ggatgtttgc
     2161 tgaacgactg gcgccgggct atcaggcgca taaccatcag caatagcatt ctgttccggc
     2221 gtttgcgggg caacgttttc cggattagtg gaaaccggtt tgctcagcgc attgttcagt
     2281 gccagcagat cctgctcgtt caacgtaccc agagctgact taatattcaa ctggttaatc
     2341 aggtagttat aacgcgcatt tgccagctct tgcttagcgt tgtacagcgt ggtggtcgca
     2401 tccaacacat caacaatggt acgcgtaccg accgagtaac ccgcttccat cgcgtctaat
     2461 gagctttgag cggaaactac ggcttgtttg taggcgttaa tgctactgat agaagcatta
     2521 atgttgttga aggaggaacg tacggtttgc acgacgctac gatgcgcgct ttccagttgc
     2581 tcgctggcac caacaaagtt gtactgggcc tgtttcacct gcgagttaac cattccgccc
     2641 tgataaatcg gcagcgagaa gctcaggcca actttgttct ggcccatatt gctatcgtca
     2701 tactgggtac cagcggcacc acgggttttc gaaccgctat aagaggtgtc agaaatcccg
     2761 gtagaagccg ttaaatccag agtcggtaag tgaccatcct gcgcctggcg aatttgctcg
     2821 cgcgccaggt cctggctcaa gcgcgcctgt aacaacgaca ggttgcgttt ttcggcttct
     2881 ttcagcagcg tgttaaccgg ctgtggtttg tcggttttaa agttttcgac attcagcgcc
     2941 gccagttccg gatagtagtt accggtgatc tggcgcagct gctctaccgc gttatcaagg
     3001 ttattacgtg cggtcacttc gttcgccagc acggtatcgt actgtgcgcg ggcgttctgc
     3061 acgtcggtga tcgctaccag gcccacgtta aaacgttggg tggtttgatc taattgacgg
     3121 tagatcgctt ctttttgcgc ctgtgtatag gaaagaacgt caatcgcatt caacacgttg
     3181 aaataagcgg tcgcggtgtt gaggatcaag gtttgttgat cggtctgata cgtgacgtcc
     3241 tgaatcccag ctgctttttc ctgcagcgtt aacgcacgcc atttcgacat atcaaaaatg
     3301 gattgagtta actgcaggga cgcactggtc gcgttagagt tgatgccgtt cgcgtcgcgg
     3361 tagccgttgc tataggtgta atctgcacct aaacctagct gtggcagtaa tggactgcgc
     3421 gcttcattaa ttttttcaaa ggcagcatca cgatcggcgg cagacttacg caattccggg
     3481 ttactaaggc gtgcttgctg ataaacttgc atcaggttct cggcctggct caacgaactg
     3541 aacccagaga ggctcaggcc gataagaatg gggagcaatt tcttcatttg cattccttgt
     3601 ggtgaagcag tatttagcgc gatcaaactg taaaattatt gcggattcta gcagaagccg
     3661 ctaccgcaaa aggttggcgt tacgtgccat agggcgttaa tgtgcctcaa tttaacacat
     3721 gactgctcaa acggcagtca aacgtcgctg aaattcacat ttaattcact attagtgcca
     3781 ggacattaac aatgcttaag ccagacaacc tgcccgttac atttggcaaa aacgatgtag
     3841 aaattattgc acgagaaaca ctttatcgcg gctttttttc attagatctt tatagatttc
     3901 gtcatcgtct attcaacggg caaatgagtc atgaggtacg gcgggaaatt tttgagcgcg
     3961 gtcacgccgc agtcttgcta ccctttgacc cagtacgtga tgaagttgtg ctgattgagc
     4021 agattcggat tgccgcgtac gacaccagcg aaaccccctg gctactggag atggttgccg
     4081 ggatgattga agagggtgaa agtgtggaag atgttgcccg tcgcgaagcg attgaagagg
     4141 cgggactgat agtcaaacgg accaaaccgg tgttaagttt cctggcaagc ccggggggca
     4201 ccagtgagcg ttcgtcaatt atggtgggcg aagtggacgc cacgaccgca agcggtattc
     4261 acggtctggc tgatgaaaac gaagatattc gcgttcatgt ggtaagccgg gaacaggcat
     4321 accagtgggt agaagagggg aaaatcgaca acgcagcgtc ggtcatcgct ttgcaatggc
     4381 tgcagctgca tcatcaagcg ttaaaaaatg agtgggcata aatgaagcgt tacacacctg
     4441 actttcctga aatgatgcgc ctgtgcgaga tgaacttttc acaattgcgc cgtttgttac
     4501 cgcgcaatga cgcacccggc gaaactgtaa gctatcaggt ggcaaacgca caatatcggc
     4561 tgacgattgt ggaatcgacc cgatacacta ccctggtgac aatagaacag actgcgcccg
     4621 cgatcagtta ctggagcctt ccgtcaatga cggtgcgtct gtatcatgac gcgatggtgg
     4681 ctgaagtgtg ttcaagccag cagatttttc gcttcaaagc gcggtatgat tatcctaata
     4741 aaaagttgca tcaacgcgac gaaaagcatc aaattaatca gtttttagcg gactggttgc
     4801 gatactgttt agcacatgga gcgatggcga ttccggttta ttagcgtcgt gaaacctaag
     4861 gacaccattt ggaaagcctg ttaacccttc ctctggctgg tgaggccaga gtcaggattt
     4921 tacaaattac cgacactcac ctgtttgcac aaaagcacga agccctgtta ggggtaaaca
     4981 cctgggaaag ctaccaggcg gtgctggagg cgattcggcc acaccagcac gaattcgacc
     5041 tgattgtcgc gacaggtgat ttagcgcagg atcaatcctc tgcggcctat cagcatttcg
     5101 ctgaaggcat cgcaagtttt cgtgcgccct gcgtctggct gccgggcaac cacgatttcc
     5161 agcccgcgat gtacagtgcg ttacaggatg cgggtatctc cccggcgaag cgcgtgttta
     5221 ttggtgagca atggcaaatc ctgttgctgg atagccaggt gtttggcgtg ccgcacggtg
     5281 agctgagcga gtttcagctt gagtggctgg aacgtaaact ggccgatgcg ccaggacgcc
     5341 atacgttgct gctgctgcat catcatccgc tacctgcagg ttgtagttgg cttgatcaac
     5401 acagcctgcg taacgcgggc gaactggata ccgtgctggc gaagtttccg cacgtcaaat
     5461 acttgctgtg cggtcacatt catcaggagc tggatctcga ctggaacggt cgccgcttgc
     5521 tggcaacgcc gtcgacctgt gtgcagttta agccgcactg ttctaacttt acgctggaca
     5581 ccatcgcgcc cggctggcgt actctcgagt tacatgctga tggcacgctg accaccgagg
     5641 tgcatcgcct ggcggacgca cgcttccaac ctgataccgc ttcagaaggc tactgatgtc
     5701 tacgcttctt tatttacacg gtttcaacag ctcgccgcgc tctgcaaaag cgagcttgtt
     5761 aaaaaactgg ctggcggaac atcaccctga cgttgagatg atcattccgc agttgccccc
     5821 gtatccttct gacgcggcag aactgctgga atccattgtc ctggaacatg gcggcgattc
     5881 gctgggtatt gtcggttcgt cactgggggg atattacgcc acctggttgt cgcaatgttt
     5941 tatgctgccc gcagtggtgg tcaacccggc ggtgcgcccg tttgaactgc tgacggacta
     6001 tctcggtcag aacgagaacc cctacaccgg gcagcaatat gtgctagagt cacgccatat
     6061 ttacgatctc aaagtcatgc agattgaccc gctggaagcg ccggatttga tctggctgct
     6121 gcaacagacg ggagatgaag tgctggatta ccgccaggcg gtggcgtact acgcttcctg
     6181 ccgccagact gtcatagaag gcggcaacca cgcattcacg ggcttcgaag attatttcaa
     6241 cccgatcgtc gattttcttg gtctgcacca tctctgacga tcaactcgaa ttgctaactt
     6301 aaaccatgac gcaaacttat aacgctgatg ccattgaggt actcaccggg cttgagccgg
     6361 ttcgccgccg tccggggatg tataccgata ccactcgccc taaccatttg gggcaagaag
     6421 ttattgataa cagtgtcgat gaagcactgg cgggccacgc aaaacgcgtg gatgtaatct
     6481 tacatgccga ccagtcgtta gaagtgattg acgatgggcg cgggatgccg gtagatattc
     6541 acccggaaga gggggtaccg gcggttgaac tgattctttg ccgtctgcac gcgggcggta
     6601 aattctctaa caaaaattac cagttctctg gcggcctgca tggcgtgggg atttcggtag
     6661 ttaacgccct gtcgaagcgc gtagaagtta acgtacgccg cgatggtcag atctataaca
     6721 tcgcctttga aaatggcgaa aaggtgcaag atttacaggt tgtcggcact tgcggtaaac
     6781 gcaataccgg taccagcgtg cacttctggc cggatgaaac cttctttgac agcccgcgtt
     6841 tttctgtttc acgcctgacg catgtgctga aagccaaagc ggtactgtgc ccgggcgttg
     6901 agatcacttt taaagatgag atcaacaaca ccgaacagcg ctggtgctat caggacggtc
     6961 tgaatgatta cctggcggaa gcggtaaacg gtttaccgac gctgccagaa aaaccgttta
     7021 tcggtaattt cgctggcgat actgaagcgg tggactgggc gctactgtgg ctgccggaag
     7081 gcggtgaact gctgaccgaa agctacgtca acctgatccc aacgatgcag ggcggtaccc
     7141 atgttaatgg cctgcgtcag ggcctgctgg acgcgatgcg tgagttctgt gaataccgca
     7201 acattctgcc gcgtggtgta aagctgtcgg cggaagatat ctgggatcgc tgcgcctatg
     7261 tgctgtcagt aaaaatgcag gatccgcagt ttgccgggca gaccaaagaa cgtctctctt
     7321 cgcgtcagtg tgcggcattc gtttcgggcg tggtgaaaga tgccttcatc ctgtggctga
     7381 accagaacgt tcaggcggcg gagctgttgg cagagatggc gatttccagc gcccagcgtc
     7441 gtatgcgtgc ggctaaaaaa gtggtgcgta aaaagctaac cagcggcccg gcgctgcctg
     7501 gtaaattggc tgactgtacc gcgcaggatc ttaaccgtac cgaactattc cttgtggaag
     7561 gtgactccgc aggcggatct gccaagcagg cgcgcgatcg cgaatatcag gcgatcatgc
     7621 cactgaaagg taagatcctt aacacctggg aagtctcttc cgacgaagtg ctggcttcgc
     7681 aggaagtgca cgatatttcg gtagctatcg gtatcgatcc tgacagcgac gatttgagcc
     7741 agcttcgtta cggcaagatc tgtatcctgg cggatgctga ctccgatggt ctgcacattg
     7801 ccacgctgct ctgcgctttg tttgtaaaac acttccgcgc attggtgaaa cacggtcacg
     7861 tttacgttgc actgccaccg ctctaccgta ttgacctcgg gaaagaggtt tattacgcgc
     7921 tgacggaaga agagaaagag ggcgtacttg agcaattaaa acgcaagaaa ggcaagccaa
     7981 acgtccagcg ttttaaaggt ctcggggaaa tgaacccgat gcaattgcgc gaaaccacgc
     8041 ttgatccgaa cactcgccgt ctggtgcagt tgactatcga tgatgaagac gatcagcgta
     8101 ctgacgcgat gatggatatg ctgctggcga agaaacgctc ggaagatcgc cgcaactggt
     8161 tgcaagaaaa aggcgacatg gcggagattg aggtctgata atcttaaccc ggcaaacatg
     8221 tgttttgccg ggttgtcgtt gattatttta ttgggcacag tttattacga tctggaaata
     8281 gtgttgccag cgaaccagcc agagttttcc agtccgtagc cattttttct gcttgtgcag
     8341 ttgttattac tgcaccttgt tgtttaataa gctcatcctc cactctggta tagtgaataa
     8401 tggtaaaatc cgtagaggac catttatcct tagagaacat cttcgtcatc gcatgaatgt
     8461 ttgcttgccc gtcatcgata aaaacaatcg cgtcatattt gttccccatt ttatccagca
     8521 tataatcgag aattacgcct ttatcttgcc cggaagaaaa aaatcccccc tggctgtaca
     8581 gccatgaccg tttgagtttc cctttttcaa agggtggcaa agtatcctct ttcttcctga
     8641 gtgcggaaga cgaaaaatca atgccgtttc gctttaattc ccgcaatgtc ggaaagagag
     8701 tatccggtgc acgggatgtc agagcaaaag cggtatgctt ttgttgccac tgttttaaca
     8761 ttgtcggtaa ctgtggctcg gtaagctgca tagggctaag ttcgtaaagc atactgattg
     8821 tattttcata caggcaagga actttttgcg agtcatccgg ctttaaaggc aatttatcgg
     8881 tttgccattg ataccagata tcaccgccaa tgcgagtgcc actcgttaat aacgtgttat
     8941 caatgtcaaa aaccaataat atcttatcgg atggatagag agtttccttt tctgtgataa
     9001 ttttactgac cgcactatat tcgccagtaa ctatattttt tacctgtgcg taagataaag
     9061 caggcagagc agagagcatc atccagagaa tgattttaaa tttattattc attttttatt
     9121 ccagattaga aagtataagt cagaccccag cgccagcgga tttggcgatt acttgatgtt
     9181 ttatccacac ctttataatc cgcaatactc atatagggta tccacgatcc cattttgtac
     9241 cctactttaa atgatgcggt atagttttta tggccatcat gccaggttaa ataaccatta
     9301 ttgtaataga ccaggtttaa ctgatattgg aagttggttt tttgtggtcg atagtcgatc
     9361 cacacatcgt aacggcggtt attacgatca tcgttgccat aacctttgga tttatcggtg
     9421 gctttatcca tgtcataacg aaaccgaccc gaaactttcc attctttgct gaagtcataa
     9481 cccgcagaaa gaccatattc gtaagttgac caatattgcg tggtatcaag ccagaacatg
     9541 ggttctaacc agaacttttt gtcatccagc ggcttaaagc ggtaaacaac agataatcct
     9601 gttccgttat tgccaaattg tgaggggtcc cacttatctt tatcaccttc atcggtgtta
     9661 tagcgtaatt ctcccatcaa accgatacca ttactaaaaa atgtaccaaa ttcaacgcgg
     9721 tccgcgtggg tgcgagtatc atcaagatac tcatgacgat aatcaataaa tccatgcggc
     9781 tgttctgcac agactagtgt aggtgacagg caggataaca ttgttattcc tgcaagtgat
     9841 attttaagat aagaattcat aatattccct ttcagaataa aatatatttt tggtgatcga
     9901 atacaccatc tgatgattcc atgtattaga atgtttttgt atctatagtt tgactttaaa
     9961 t
//
LOCUS       DAZFRG010000180         9841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFRG010000180 DAZFRG010000000
VERSION     DAZFRG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.181"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            216..1373
                     /gene="acrE"
                     /locus_tag="ACHKUC_003595"
     CDS             216..1373
                     /gene="acrE"
                     /locus_tag="ACHKUC_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO6619788.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSTKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1385..4489
                     /gene="acrF"
                     /locus_tag="ACHKUC_003596"
     CDS             1385..4489
                     /gene="acrF"
                     /locus_tag="ACHKUC_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO6619789.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIILVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4742..4963
                     /locus_tag="ACHKUC_003597"
     CDS             4742..4963
                     /locus_tag="ACHKUC_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619790.1"
                     /translation="MKRLIPVTLLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5394..6419
                     /locus_tag="ACHKUC_003598"
     CDS             5394..6419
                     /locus_tag="ACHKUC_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6619791.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6487..7668
                     /gene="yhdX"
                     /locus_tag="ACHKUC_003599"
     CDS             6487..7668
                     /gene="yhdX"
                     /locus_tag="ACHKUC_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6619792.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALLWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7678..8781
                     /gene="yhdY"
                     /locus_tag="ACHKUC_003600"
     CDS             7678..8781
                     /gene="yhdY"
                     /locus_tag="ACHKUC_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6619793.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWVFLQANWVGSTRADCTKSGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRSRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8789..9547
                     /gene="yhdZ"
                     /locus_tag="ACHKUC_003601"
     CDS             8789..9547
                     /gene="yhdZ"
                     /locus_tag="ACHKUC_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO6619794.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDDFFAHPKSERTRAFLSQVIH"
     gene            complement(9610..9725)
                     /gene="rrf"
                     /locus_tag="ACHKUC_003602"
     rRNA            complement(9610..9725)
                     /gene="rrf"
                     /locus_tag="ACHKUC_003602"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9765..9840)
                     /locus_tag="ACHKUC_003603"
     tRNA            complement(9765..9840)
                     /locus_tag="ACHKUC_003603"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9805..9807),aa:Thr,seq:ggt)
BASE COUNT         2338 a         2357 c         2523 g         2623 t
ORIGIN      
        1 attttaattc tatttctttc attccgcgtc aaaacaaaac agtagaatat taatcttttt
       61 ttgtgtttat gtgccttgag ttgccagtat tcataactat tccttacatc gacgaatgat
      121 aatttgtagg atagcgaact gtatttttct ttctgcgagt taacgcattg cctttttggg
      181 caaataacgc gcttttggtt ttttgaggaa tagtaatgac gaaacatgcc aggtttttcc
      241 tcctgccctc ctttattctg atctccgcgg ctttaatcgc cggttgtaac gataagggag
      301 aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca tattgtgaaa acggccccgt
      361 tagaagttaa gactgaatta ccaggccgca ccaatgctta ccgtatagcc gaagttcgcc
      421 cacaggttag cgggatcgta ctgaatcgca atttcactga aggcagcgat gtgcaagcag
      481 gccagtccct gtaccagatc gatcccgcga cctatcaggc aagttatgac agcacgaaag
      541 gcgaactggc gaaaagtgaa gccgccgccg ccatcgcgca tttgacggta aaacgttacg
      601 ttccgctcgt gggtacgaaa tacatcagtc agcaggagta cgaccaggcc attgctgatg
      661 ctcgccaggc cgatgccgcc gtgattgccg caaaagccac agtcgaaagc gctcgcatca
      721 atctcgctta taccaaagtg actgcgccaa ttagcggacg tatcggcaaa tcgaccgtga
      781 ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact ggcgactgtc cagcagctcg
      841 atcctatcta cgttgatgtg acccaatcca gcaacgattt tatgaggctg aagcaatccg
      901 tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa cgtagagttg gtcatggaga
      961 acggtcaaac ctatcccctg aaaggtacgc tgcaattctc cgatgtgacc gttgatgaaa
     1021 gcaccggctc cataacccta cgtgctgtct tccctaaccc gcaacatacg cttttgccgg
     1081 gtatgtttgt gcgcgcacgg attgatgaag gcgtccaacc tgacgccatt cttatcccgc
     1141 aacaaggcgt tagccgcaca ccacgtggtg atgcaaccgt gctgattgtt aacaataaaa
     1201 gtcaggttga agcgcgccct gtcgttgcca gtcaggcgat tggcgataaa tggttgatta
     1261 gcgaaggtct gaaatctggc gatcaagtca ttgtcagcgg cctgcaaaaa gcgcgtccgg
     1321 gagagcaggt taaagccact accgataccc ccgcagatac tgcatcgaag taaggcaatc
     1381 tgacatggca aactttttta ttcgacgacc gatatttgca tgggtgctgg ccattattct
     1441 gatgatggcg ggcgcactgg cgatcctaca attgcccgtc gcccagtatc caaccattgc
     1501 gccgcctgcg gtttctgttt cggcaaacta tccgggtgct gatgcgcaga ccgtgcagga
     1561 tacagtgacg caggttatcg aacagaatat gaacggtatc gataacctga tgtatatgtc
     1621 ctccaccagc gattccgccg gtagcgtgac aattaccctt accttccagt ccgggaccga
     1681 tcctgatatc gcgcaagtgc aggtgcagaa caaactccag ctcgccacgc cgttgctgcc
     1741 gcaggaggtt cagcagcagg ggatcagtgt tgaaaagtcc agtagcagct atttgatggt
     1801 ggcgggcttt gtctctgata acccaggcac cacacaggac gatatctcgg actatgtggc
     1861 ctctaacgtt aaagatacgc ttagccgtct gaatggcgtc ggtgacgtac agcttttcgg
     1921 cgcacagtat gcgatgcgta tctggctgga cgccgatctg ctaaacaaat ataaactgac
     1981 accggttgat gtgattaacc agttgaaggt acagaacgat cagatcgctg ccggacagtt
     2041 gggcggaacg ccagcgttac cagggcaaca gttgaacgcc tcgattattg ctcagacgcg
     2101 gttaaaaaat ccggaagaat tcggcaaagt gaccctgcgc gtaaacagtg acggctcggt
     2161 ggtacgcctg aaagatgtcg cacgggttga acttggcggt gaaaactata acgttatcgc
     2221 tcgtatcaac ggaaaaccgg cggcgggcct ggggattaag ctggcaaccg gcgcgaatgc
     2281 tctcgatacc gcgaaagcca ttaaggcaaa actggcggaa ttacagccat tcttcccgca
     2341 gggaatgaag gttctctacc cttatgacac cacgcctttc gtccagcttt ctatccagga
     2401 agtggtgaaa acgctgttcg aagccattat cctggtattc ctggtgatgt atctgttctt
     2461 gcagaacatg cgggcaacat tgatacccac cattgctgta cctgtcgtgt tgttagggac
     2521 atttgccata ctcgccgctt ttggttactc catcaatacg ctaacgatgt ttgggatggt
     2581 acttgccatc ggacttcttg ttgatgatgc gatagtcgtt gtggagaacg tcgagcgcgt
     2641 aatgatggag gataaactcc cccccagaga agcgacggaa aaatcgatgt cgcaaatcca
     2701 gggcgcgctg gtaggtatcg cgatggtact gtcggcggta tttattccga tggcattctt
     2761 cggtggctca acaggggcaa tttatcgaca gttctctatc actattgttt cagcaatggc
     2821 gctttcagta ctggttgcgc tgattctcac ccccgcgtta tgtgcaacat tgctgaaacc
     2881 agtctctgcc gagcatcacg aaaataaggg aggattcttc ggctggttta acaccacttt
     2941 cgatcatagc gttaatcact acaccaatag cgttggcaaa atcctcggtt ccacagggcg
     3001 atatttactg atctatgcgc tgattgttgc aggaatggtg gtgttgtttt tacgtcttcc
     3061 gtcttccttc ttacctgaag aggatcaggg tgtctttctg accatgattc agttacccgc
     3121 tggcgcgacg caagagcgga cgcaaaaagt gttggatcaa gtgacggatt actatctgaa
     3181 gaacgagaaa gcgaacgttg aaagtgtctt tacagttaac ggctttagct tcagcggcca
     3241 ggcacaaaac gccggtatgg ccttcgtcag tctgaaaccg tgggaagagc gtagtggtga
     3301 cgaaaacagt gcggaagcag taatccatcg tgcaaaaatg gaattgggca agatccgcga
     3361 cggttttgtc attccattta atatgccagc cattgttgaa ctaggcacgg caacgggttt
     3421 cgactttgag ttaattgatc aggctgggct gggtcacgat gccctgaccc aggcccgtaa
     3481 ccagttgctt ggcatggcgg cgcaacatcc tgccagctta gtcagcgtgc gccctaatgg
     3541 cctggaagac accgcgcagt ttaaactgga agttgaccag gaaaaggcgc aggcattagg
     3601 tgtttcactt tctgacatca atcagaccat ttcaacggcg ttgggtggga cttacgttaa
     3661 cgacttcatc gaccgtggtc gcgtgaaaaa ggtgtatgtt caggcggatg ccaaattccg
     3721 tatgctgcca gaagatgtcg ataaacttta tgtccgcagc gccaacggcg aaatggtgcc
     3781 attctctgcc tttaccactt cacattgggt gtatggctct ccgcgactgg aacgctacaa
     3841 cggtctgccg tcaatggaga ttcaggggga agccgcgcca ggaaccagtt ccggcgatgc
     3901 tatggcgttg atggaaaacc ttgcgtcaaa attaccggcg ggcattggtt atgactggac
     3961 gggtatgtca tatcaggaac gtttatcggg aaaccaggct cccgctctgg tagcaatttc
     4021 ctttgtggtt gttttcctgt gccttgctgc actctatgaa agctggtcaa ttcctgtctc
     4081 ggttatgttg gtcgtgccgt tagggattgt cggcgtgcta ctggcggcga cactctttaa
     4141 tcaaaaaaat gacgtctact ttatggtggg cttgctaacg acaattggct tgtcggctaa
     4201 aaacgctatt ttgattgttg agttcgctaa agatctcatg gagaaagagg gtaaaggtgt
     4261 tgttgaagcg acactaatgg cagtacgtat gcgtctgcgt cctatcctga tgacctctct
     4321 cgcctttatt ctcggcgtat taccgctggc tatcagtaac ggggccggca gtggcgcgca
     4381 aaacgcagtc ggtatcgggg taatgggcgg aatggtctct gcaacgttgc tggcaatctt
     4441 cttcgtgcca gtgttctttg tggtgatccg tcgatgcttt aaaggataaa tcagaaacat
     4501 aaaggcgctt tcaggcgcct tttttatttt cagtgaaccc cataaaaatt aataagatat
     4561 tcttcttctc actctttaaa aactttctat agttcccgct cccttcacta tttttacaat
     4621 tcacataaat tgtcattatt cattccgcaa gatttataac ctccagtgat aaaatggcat
     4681 tcagctcgtt aataagagag ttaacttatt aagcgttagc gtttattact gaggtaacac
     4741 catgaaaaga ttaattcctg tgacattgct caccgcattg ctggcgggct gtgctcacga
     4801 ttcgccctgc gttccggttt atgacgatca gggtcgtctg gttcacacca atacctgtat
     4861 gaaaggcacg actcaggata actgggaaac ggctggggct attgccggcg gggcagcagc
     4921 tgttgctggc ctgacgatgg ggatcatcgc tctgtcgaaa taactaaaca gggcgcggag
     4981 ctccgcgctc tttttaacga tacagtatct ttccataaga taaaatattg ctatattctg
     5041 ctgggtaatt ccctgctgat ttagcatgtt atatcacgtt atttcgctgc tttcgaaata
     5101 acgcatcaat ttaactttca cacatctcac cagaaatata ctcacgctaa caatcattat
     5161 tctgcttagt aaaaaacgcg taaatatttt gcgccaaaat gtggcgcatg tttcattttc
     5221 gcaccattgc ggggcgctgt ttttattatt tcctgtctac actcagctta ttgcgacgcg
     5281 aaatcgtgcg caaaagctgg cactactttt gcttataaga ggatggccac agacaggtaa
     5341 aagacgtttc ccaaacgtcc tataacgata attttcgcct cacaggaagc attatgaaaa
     5401 agatgatgat agccacactg gctgccgcca gcgtgctgct tgccgttgca aatcaggcgc
     5461 atgctggcgc gacgcttgat gccgttcaga aaaaaggttt tgtgcaatgc gggatcagtg
     5521 atggattacc tgggttctct tatgccgatg ctgacggtaa gttttcaggt attgatgtag
     5581 atgtttgtcg tggtgttgcc gctgctgtat tcggtgacga tacgaaagtg aaatataccc
     5641 cgctcactgc aaaagaacgc ttcaccgctt tacagtcagg ggaggtggat ttgctctccc
     5701 gtaatacgac ctggacttca tctcgcgatg ccgggatggg aatggcattt actggcgtca
     5761 cttattacga cggcattggc ttcctgacgc acgataaagc ggggctaaaa agcgcgaaag
     5821 aactggatgg cgctaccgtc tgtattcagg cggggactga taccgaactc aacgtcgccg
     5881 attacttcaa ggcaaataat atgaagtaca cgccagtaac cttcgatcgc tctgacgaat
     5941 cagcgaaggc actggaatct ggtcgctgcg atacgctggc ctcggatcaa tcacaactgt
     6001 atgccctgcg catcaaatta agcaacccgg ctgaatggat tgtcttaccg gaagttatct
     6061 ccaaagaacc gcttgggccg gtagttcgtc gtggcgatga tgaatggttc tcgattgtac
     6121 gctggacgct tttcgccatg ctgaatgctg aagagatggg catcaattcc cagaacgtcg
     6181 atgaaaaagc ggctaatccg gcaacgcctg atatggcaca tctgctgggt aaagaaggcg
     6241 attacggcaa ggatctgaag ctggataata aatgggctta caacatcatc aaacaggtgg
     6301 gtaactactc ggaaattttt gagcgtaacg taggttcaga aagcccgctg aaaattaaac
     6361 gtgggcaaaa taatctctgg aataacggcg gaattcagta cgcgccgccc gtgcgttaag
     6421 tcggtgtgag gtaacgagcg tcgctacggc ggcgctctct tcagcgttat gctttccgag
     6481 gtttctatgt ctcatcgccg ctcaaccgtt aaaggctcac tctcctttgc caaccctacg
     6541 gttcgcgcct ggttattcca gatccttgcc gttgttgctg ttgttggcat tgttggttgg
     6601 ctatttcaca acactgtgac gaatctcagt aatcgtggca ttacttcagg ttttgccttt
     6661 ctggatcgcg gcgctggctt cggtattgtc cagcatttga tcgattacca gcagggtgat
     6721 acctacggac gcgttttcat tgttggctta ctcaatacgc tactggtttc tgcattgtgt
     6781 attgtattcg cttctgttct gggcttcttt atcggtctgg cgcgactttc ggataactgg
     6841 ctgctacgaa agctttccac aatttatatt gagatcttcc gtaatattcc gccattgctg
     6901 caaatcttct tctggtactt tgccgtattg cgcaatttgc ccggaccacg acaggcagtg
     6961 agcgcgtttg atctcgcctt tttgagcaat cgtgggcttt atattccgtc gccgcagctg
     7021 ggagacggat ttattgcgtt tatcctcgct gttgttatgg ctatagtcct ttcagttggg
     7081 ctattccgct ttaataaaac ataccagata aagaccggac aactgcgccg cacctggcca
     7141 ttcgccgctg tgttgatcat tggtttgcct ttactggcgc aatggctttt tggcgcagca
     7201 ctactctggg atgtcccagc ccttcgaggc tttaatttcc gcggcgggat ggttttaatt
     7261 cctgaactgg cagccttaac gctggcgctt tcggtttata cttctgcatt tatcgccgaa
     7321 attatccgcg cagggatcca ggcagtgcct tatggtcaac atgaagcagc tcggtcactg
     7381 ggattaccca acccggtcac gctacgccag gtcattattc cccaggcatt gcgggtgatt
     7441 attccaccgt taaccagcca gtatctcaac atcgtcaaga actcctctct tgccgccgct
     7501 attggctatc ccgatatggt ttcgctgttt gctggcaccg tgctgaacca aacggggcaa
     7561 gccatcgaga cgatagccat gaccatgtcg gtctatctga ttatcagcct gactatctcg
     7621 ctgctgatga atatctataa ccgccgcatc gcgatcgttg aacgctaagg agtcatgatg
     7681 acaaaagtat tgctgtctca tcccccgcgc ccggcgagcc ataactcaag ccgcgcgatg
     7741 gtgtgggtgc gaaaaaatct gttctccagc tggagcaata gcctgctgac tattggctgc
     7801 atatggttga tgtgggaact gatcccaccg ttgctgaact gggtattttt gcaggctaac
     7861 tgggttggct caacgcgtgc cgactgcaca aaatccggtg cctgttgggt cttcatccac
     7921 gaacgatttg gtcagtttat gtatggactt tacccacacg accaacgctg gcgaattaac
     7981 ctagcattac tgattggcct tgtttctatc gcaccaatgt tctggaaaat acttccgcat
     8041 cgcagtcgct atattgcggt ctgggcggtg atttacccac tggttgtctg gtggctgatg
     8101 tatggtgggt ttcttggtct tgatcgggtt gaaacccggc aatggggcgg gttgacgcta
     8161 actttaatta ttgcatcagt tgggattgcg ggggcgctac cgtgggggat cttactggcg
     8221 ttaggtcgcc gctcccatat gccgattgtg cgtatcttat cggtcatttt tatcgagttc
     8281 tggcgcggcg taccgctgat taccgtattg tttatgtctt cggtcatgct gccgttgttt
     8341 atggcagaag gcaccagtat cgacaaactg atccgcgcgc tggttggcgt gatcctgttt
     8401 cagtcagcat atgttgcgga agtcgtgcga ggcggattgc aggcgctgcc taaagggcaa
     8461 tatgaagcgg cagagtcgct ggcattgggt tactggaaaa ctcaggggct ggttattctg
     8521 ccacaggcgt tgaagctggt aattcctggg ctggtaaata ccatcatcgc actcttcaaa
     8581 gataccagtc tggtgatcat catcgggttg ttcgatcttt tcagtagcgt tcagcaggca
     8641 accgttgatc ccgcctggtt gggtatgtcg acggaagggt atgttttcgc cgcgctgatc
     8701 tactggatct tctgtttcag catgtcacgt tatagccaac atctggaaaa acgttttaac
     8761 accgggcgta caccgcattg aggacactat gagccaaatt ttactgcaac ctgctaacgc
     8821 gatgattacg ctggaaaacg tcaataaatg gtatggacaa ttccatgttt tgaaaaatat
     8881 aaatttaacc gtgcaaccgg gcgaacggat cgttctatgt ggcccttcag gttccggtaa
     8941 atcgacaacc attcgttgta ttaatcatct ggaagaacat caacagggac ggatcgtggt
     9001 agatggcatc gaacttaatg aagatatccg caatattgag cgcgtcaggc aggaagtggg
     9061 aatggtcttt cagcatttca atctcttccc tcatctgacc gtgttacaga actgtaccct
     9121 ggcaccgatt tgggtacgca agatgccaaa gaaagaggct gaagctctgg cgatgcatta
     9181 ccttgagcgg gtgagaattg ccgaacatgc ccagaagttt cccggacaga tttctggtgg
     9241 tcagcagcaa cgtgttgcca tagcacgttc gctgtgtatg aagccgaaaa ttatgttgtt
     9301 tgatgagcca acgtcggcgc tcgatcctga gatggtgaaa gaggtgctgg atacgatgat
     9361 tgggctggcg cagtcgggta tgacaatgtt gtgtgtaaca catgagatgg ggtttgcacg
     9421 aaccgtcgct gaccgggtga tttttatgga tcgtggggaa atagtggagc aagctgcacc
     9481 tgatgacttt tttgcgcatc ctaaatcaga gcgtacgagg gcatttttat cgcaggtaat
     9541 ccattaattg aatgttagtt cgaaaagcaa aaaggccatc ctttcggatg gccttttgct
     9601 tgatttgatg tctggcagtt ccctactctc gcatggggag accccacact accatcggcg
     9661 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg
     9721 ccagacaaat tcttttctaa tctgccgaac tttaacctaa aaagtggtgc tgatacccag
     9781 agtcgaactg gggacctcac ccttaccaag ggtgcgctct accaactgag ccatatcagc
     9841 a
//
LOCUS       DAZFRG010000181         9730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFRG010000181 DAZFRG010000000
VERSION     DAZFRG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.182"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(48..123)
                     /locus_tag="ACHKUC_003604"
     tRNA            complement(48..123)
                     /locus_tag="ACHKUC_003604"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Lys,seq:ttt)
     gene            complement(127..202)
                     /locus_tag="ACHKUC_003605"
     tRNA            complement(127..202)
                     /locus_tag="ACHKUC_003605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Val,seq:tac)
     gene            complement(254..329)
                     /locus_tag="ACHKUC_003606"
     tRNA            complement(254..329)
                     /locus_tag="ACHKUC_003606"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Lys,seq:ttt)
     gene            complement(332..407)
                     /locus_tag="ACHKUC_003607"
     tRNA            complement(332..407)
                     /locus_tag="ACHKUC_003607"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(372..374),aa:Val,seq:tac)
     gene            complement(443..518)
                     /locus_tag="ACHKUC_003608"
     tRNA            complement(443..518)
                     /locus_tag="ACHKUC_003608"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Lys,seq:ttt)
     gene            complement(683..1474)
                     /gene="cpoB"
                     /locus_tag="ACHKUC_003609"
     CDS             complement(683..1474)
                     /gene="cpoB"
                     /locus_tag="ACHKUC_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO6619795.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGAANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1484..2005)
                     /gene="pal"
                     /locus_tag="ACHKUC_003610"
     CDS             complement(1484..2005)
                     /gene="pal"
                     /locus_tag="ACHKUC_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO6619796.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(2040..3332)
                     /gene="tolB"
                     /locus_tag="ACHKUC_003611"
     CDS             complement(2040..3332)
                     /gene="tolB"
                     /locus_tag="ACHKUC_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO6619797.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3465..4730)
                     /gene="tolA"
                     /locus_tag="ACHKUC_003612"
     CDS             complement(3465..4730)
                     /gene="tolA"
                     /locus_tag="ACHKUC_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6619798.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAVADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4795..5223)
                     /gene="tolR"
                     /locus_tag="ACHKUC_003613"
     CDS             complement(4795..5223)
                     /gene="tolR"
                     /locus_tag="ACHKUC_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO6619799.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5227..5919)
                     /gene="tolQ"
                     /locus_tag="ACHKUC_003614"
     CDS             complement(5227..5919)
                     /gene="tolQ"
                     /locus_tag="ACHKUC_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO6619800.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5916..6320)
                     /gene="ybgC"
                     /locus_tag="ACHKUC_003615"
     CDS             complement(5916..6320)
                     /gene="ybgC"
                     /locus_tag="ACHKUC_003615"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6619801.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6470..6763)
                     /gene="ybgE"
                     /locus_tag="ACHKUC_003616"
     CDS             complement(6470..6763)
                     /gene="ybgE"
                     /locus_tag="ACHKUC_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO6619802.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6763..6876)
                     /gene="cydX"
                     /locus_tag="ACHKUC_003617"
     CDS             complement(6763..6876)
                     /gene="cydX"
                     /locus_tag="ACHKUC_003617"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO6619803.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6891..8030)
                     /gene="cydB"
                     /locus_tag="ACHKUC_003618"
     CDS             complement(6891..8030)
                     /gene="cydB"
                     /locus_tag="ACHKUC_003618"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6619804.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8046..9614)
                     /gene="cydA"
                     /locus_tag="ACHKUC_003619"
     CDS             complement(8046..9614)
                     /gene="cydA"
                     /locus_tag="ACHKUC_003619"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO6619805.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2384 a         2669 c         2327 g         2350 t
ORIGIN      
        1 tacgggtgtt acctgaaaga tttctactcg gaaccaatgt ttaaaattgg tgggtcgtgc
       61 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga
      121 cccgagtggt gggtgatgac gggatcgaac cgccgacccc ctccttgtaa gggaggtgct
      181 ctcccagctg agctaatcac ccgatactac gctggatact acaaactact tccaccgaac
      241 ccaccgtaat cagtggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc
      301 aactgctcta ccaactgagc taacgacccg gtggtgggtg atgacgggat cgaaccgccg
      361 accccctcct tgtaagggag gtgctctccc agctgagcta atcacccgat actacgctgg
      421 atactacaac taactatgtt agtggtgggt cgtgcaggat tcgaacctgc gaccaattga
      481 ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt
      541 gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa
      601 aatttcgatg tagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg
      661 acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgcgcc tgtttagcgc
      721 catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt
      781 cacctttgtc ctgcatgatg acgccgactt taaacatcgc atctgcagcc ttcggtgact
      841 tcggatagtt tttcactacc gaagcaaaat aatacgccgc atcatctttt ttacccttgt
      901 tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat
      961 ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca
     1021 gcgcaatagc tgcattgtaa tccgtgtttg catcaccgct tttcaccggc gcgccagcat
     1081 tcgcagcacc agcatcagct gtcggcgtcg ttgatgccgc cacaccgctt tgatcgccgc
     1141 tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct
     1201 tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg
     1261 aatcaatatc ggattgatta tcagagagtt gttgctggag ttgggttaaa agctggctgt
     1321 gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct gagccgacac
     1381 tactgattgg tgcctgagca aaagcggccc aaggggccgc tataccaacc agtaacgaca
     1441 gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg
     1501 cacgacggtt tttggcatac gccgcttcgt catgacccag tactgcaggt ttttctttac
     1561 cgtaagaaac gatggagatc tggtcagcag aaacgccttt accctgcaga tacatcttaa
     1621 cagcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg
     1681 cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt gcgtgtgcat
     1741 ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt
     1801 tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt
     1861 tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc
     1921 tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc
     1981 ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat
     2041 cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct
     2101 ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg
     2161 tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac
     2221 agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccgttg
     2281 gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa
     2341 ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgcgga
     2401 cgaccggcct ggtcagaagt aaatgccagg ttctggctgt ccgggaacca ggtcggttcg
     2461 gtattgttac tgcgaccatc agttacctgg cggatctgac cagaagccaa atccattacg
     2521 tacaggttca ggctaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag
     2581 aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc
     2641 agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa
     2701 ccgtctggtg accacgccgg agacatcagc ggttgcggtg agcggtgaac gacaaactgg
     2761 ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc gttagtctga
     2821 acaacgtagg caatacgggt acggaacgca cctttaatac cggtcagttt ttcaaatact
     2881 tcgtcactgg cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc
     2941 tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc gacattgtaa
     3001 gaaccatccg gattcggagt gacctgaccg acaaccacag cgtcaatgcc cagcgcggac
     3061 catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcacgatct
     3121 aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc aatatcttca
     3181 ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg accggaatct
     3241 acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga
     3301 aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc
     3361 cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg
     3421 caaccggtgc ctgatgttga ccgtcggaac agtcaacatc gcgattacgg tttgaagtcc
     3481 aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca
     3541 agtttagctg ctgccaacgc agcttgacaa agtgcgggat cgccaccttc aggtttgata
     3601 tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag
     3661 gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc atagttattg
     3721 atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg
     3781 ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa
     3841 atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc
     3901 gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc
     3961 ttttcagcag ccgctttttc tgctgctttt ttgtcggcta ccgctttctc tgctgccgct
     4021 ttcttatcag ctgcagcctt ttcagcagcc gctttcttct cagcttctgc cttggctttt
     4081 tcagcagctt cggttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttca
     4141 gctttcttct tcagcgccgc agcagcagcc tcggcttttt tctgcgcttc gactgcggct
     4201 ttggcggctt ctgcttctgc ttttttcttc gcgtctgccg ccgctttctt cgctgcttct
     4261 tccgcagctt tagcatccgc ttcggccttc gctttagcat ctgccgccgc tttcgctgcc
     4321 gcctcttccg cttgcttctg ctttaactcg gcctgttttg cggcttcttc agcctgcttt
     4381 ttctgctcct gagccgctaa ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc
     4441 gcttgtttct cacgcagttc ttcagcagcc tgctgctcct tcatcttgcg ctgctcatca
     4501 gaacgcttcg cgcttgattc ctggctttgc atacgtttgt actgctcaac taccgcacct
     4561 gaatcaacca tgacagcgtc gatggacgaa ccaccgccac ctccggctga agcttctata
     4621 ttctcatcga acgaactcca gatcagcgcc gcaaataaga tgacatgcag cactgctgaa
     4681 ataattatcg cccgcttgag cttgtcgttt tgttcggttg cctttgacac tctcggtttc
     4741 caaaaactgt tcgcctgtta cccgctctct ttcaagcaag ggaaacgcag atgtttagat
     4801 aggctgcgtc attaaaccaa ccgatttcac acccgcacta tgtaacaagt tcagtgcttt
     4861 aattatttca tcgtaaggca catcttttgc gccaccgatc agaaagaccg ttttcgggtt
     4921 ggccttgaaa cggctggaca cttccgccac cacctgctct ggtggtaaac gctccagacg
     4981 atctttctca accaccacgg tgtactgacc aataccagac acttcaacaa tcactggcgg
     5041 attatcgtta ctgctcaccg cctgtgattc agtagcgtct ggcagatcga cctccacgct
     5101 ctgggtgatg atgggcgctg tcgccataaa gatcagcaac agcaccagca gtacatccag
     5161 caacggtaca atgttgattt cggacttgag atcgcgacga cctcgtccac gcgctctggc
     5221 catggcttac cccttgttgc tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt
     5281 aaactcttcc ataaagttgt cgtaattcag ttccagtttg tttacgcgct ggtttaggcg
     5341 gttataagcc attaccgccg gaattgcggc gaacaaacca attgccgtcg caatcaacgc
     5401 ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt tttaccgccc cgagggcgat
     5461 aaaggcgtgc atgatccccc agaccgtacc aaacagacca atatacgggc tgatggagcc
     5521 aaccgtaccg aggaaaggaa tgtgcgtttc cagattttca agttcacgat tcatggaaat
     5581 acgcatcgca cgcgacgccc cttccactac ggcttccggc gcatggctat tggcacgatg
     5641 caggcgcaca aactctttga agccgctgta aaagatttgt tccgaaccgc tcagattatc
     5701 ccgtttcccc tggctctctt gatagaggcg agagagttcg attccagacc agaatttatc
     5761 ttcaaacgct tcggcttcgc gcgccgctgc gttaagaata cgggtccgct ggataataat
     5821 ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa ccagaaggct
     5881 agccttcagg aacaaatcaa ggatattcat gtcagtcact gcttaaactc cgcgacaata
     5941 gacttgggaa gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac cagaacctct
     6001 gcttcattca acaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg
     6061 ccacgcattg atgttatttc agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg
     6121 taatattcca ccgtcatttt acgtaccaca aaggcaacgc gttcagccat cagcgcctgt
     6181 tgactgaagt gatgatgacg cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa
     6241 ctggcgtggt acaccacacc accggcatcg gtatcttcat agtagacacg aaccggccat
     6301 cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa caaaagttag agcttttaaa
     6361 cgctgctact atacgcgcgc gattgaggtt tgggaatgag agcaagttaa gggagcgtaa
     6421 aaatttatgg gcctgactcg gcccataaat ttttaaagag aattctgatt taaaagaaga
     6481 agaaaatcag cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc
     6541 agagaacctt ctgcggacga aagcccacgc cgtgaatcac accagcacag acggcccaca
     6601 taagcagtaa accatgccag atttccagtt cactggtctt cgcggcgaaa cgtgatgggt
     6661 cccaaaacat acatcctgct aacagaagtg ccatcacgaa ggaaagcgcc cgcagggggc
     6721 gcttgtccat taccgcatac aaggttgcga taatcttact catcagatgt cttcttgacc
     6781 ggctttgcct gattcgacgt gctcaagcgc cagcgcggtg attaccccaa acgaacaggc
     6841 aagaagcgtt cccagaatcc atgcgaaata ccacattttt agctccttac ttagtacaga
     6901 gagtgggtgt tacgttcaat atcttctttg gtgatacgac cgaacatttt ccagtaacac
     6961 caggcggtgt agagcagaat gatcggtacc agaaccaccg caacccaggt catgacgtta
     7021 agcgtcagct ggctggaagt tgcatcccac attgtcagac ttgcgttcat catggtgctg
     7081 gacggcatca caaacgggaa cattgcgata ccggcagtca ggatgatgca ggccagcgtc
     7141 agggaggaga acacaaacgc ccacgcggct ttatccatac gtgcagtcag gatggtcagc
     7201 agcggcagaa ccacacccag tgccggaata gcccacagaa ttggcgtgtt gttgaagtta
     7261 accagccatg cgccagcttc acgaaccact tctttattca gcgggttaga ggctgcgtaa
     7321 tggtccattg tcgatttcac gacataacca tcgataccgt acatcaccca tacgccagcc
     7381 agtgcgaagc agaccagcgt caccagtgcc gccacctgag ccgttgcacg ggtacgcagg
     7441 tgcagttcgc ccacggtacg catttgcaga taggttgcgc cctgagtaat gatcatccct
     7501 acgctcacca cacctgccag caggccgaac gggttaagca gctggaagaa gttaccggtg
     7561 tagtacagac gcagatattc atcaacgttg aatggtacgc cctgcaacag gttaccgaac
     7621 gctacaccaa tcaccagcgg cggaacgaag ctaccaatga agatgcccca gtcccacatg
     7681 ttacgccagc gggtttcttc aatcttggag cggtagtcaa aaccgaccgg acggaagaac
     7741 aaagacgcca gcacgaggat catcgccaca tagaagccgg agaacgcagc ggcatagacc
     7801 atcggccagg cagcaaacag tgcgccgcct gcagtgatca gccaaacctg gttaccgtcc
     7861 cagtgtggtg cgatggagtt aatcataatt cgacgctcgg tgtcgttacg accgaggaaa
     7921 cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga
     7981 acgccaacca gcagccacca gataaaacgc aatacttcat aatcgatcat ttgacgactc
     8041 ctgtcttagc gtgccggctg agtagtcgtg gaagactgct caaagtgata gcgaccggtt
     8101 ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatta agaacaattc tgccaccagg
     8161 aacagggtat acaggccgca aatcagcacc attgagaaga tgagatcgcc tgcggtcagt
     8221 gacgagttcg ccacagctgt cggcagcact tcaccgatag cccacggttg gcggccatat
     8281 tcagccacga accagcccgc ttctacagca atccacggca gcggaatacc gtacagcgcg
     8341 gcgcgcagca gccatttttt ctcgccaatg cggttgcgga tgacactcca gaaagagagc
     8401 gcaatgattg ccagcagcag gaagccacac gccaccatga tacggaacgc gaagtacagc
     8461 ggcgctacac gtggaatgga gtctttggtt gcctgttgaa tctgagcttc agtcgcatca
     8521 gccacgtttg gcgtatagcg tttcagcagc agaccgtaac cgagatcttt cttcatgcta
     8581 ttgaactggt cacgaacggc ctggtcggta gaaccagaac gcagttgttc gagcagagag
     8641 tacgccttca tcccgttacg aatgcgttct tcatgctgca ccatcagttc tttcagaccg
     8701 ataactggag tatccacgga acgcgttgca atgatgccca gcgcgtaagg gatctggatc
     8761 gcaaatttgt tcgtttcctc ttcctgatca ggaatgccga acagagtaaa ggcagcaggt
     8821 gcaggttgcg tttcccactc ggcttcgata gcagccagtt tggttttctg cacgtcgccc
     8881 atttcgtaac cggattcatc acccagaaca ataacagaca gaacggcagc cataccgaag
     8941 ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt cacgaccttt cagcatatac
     9001 catgcgctga taccgaggat gaacatcgcg ccagtcacat aaccagaagc tacggtgtga
     9061 acgaatttca cctgagcaac cgggttaagc accagctcgg agaagctcac catctccata
     9121 cgcatagttt caaagttgaa atcggacgcg attgggtttt gcatccagcc gtttgcaacc
     9181 agaatccaca gtgcggacag gtttgaaccg agcgccacca gccaggtgac acacatatgc
     9241 tgaactttac ccagacgatc ccaaccgaag aagaacagac ctacaaaggt ggattcgagg
     9301 aagaaggcca tcagaccttc gattgccagc ggcgcaccga agatatcccc tacatagtgg
     9361 gaatagtaag accagttagt cccgaactgg aactccatgg tcagaccggt agccacaccc
     9421 agagcaaagt tgataccaaa caacttgccc cagaacttgg tcatatcttt ataaatctgt
     9481 ttgccggaaa ggacgtagac cgtttccata atggccagca ggaacgccat accgagcgtc
     9541 agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg
     9601 actatatcta acatcatgac tccttgctca tcgcatgaag actccgagag tgaaccccgt
     9661 tagaaagggt cacacgcatg ccccaataca aaattatttg ctcgcctcta tcgctgccat
     9721 ttcatcaaaa
//
LOCUS       DAZFRG010000182         9711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFRG010000182 DAZFRG010000000
VERSION     DAZFRG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.183"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..244)
                     /gene="tnpA"
                     /locus_tag="ACHKUC_003620"
     CDS             complement(<1..244)
                     /gene="tnpA"
                     /locus_tag="ACHKUC_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO6619806.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYL"
     gene            complement(424..1962)
                     /gene="emrB"
                     /locus_tag="ACHKUC_003621"
     CDS             complement(424..1962)
                     /gene="emrB"
                     /locus_tag="ACHKUC_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO6619807.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRIGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(1979..3151)
                     /gene="emrA"
                     /locus_tag="ACHKUC_003622"
     CDS             complement(1979..3151)
                     /gene="emrA"
                     /locus_tag="ACHKUC_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO6619808.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(3278..3808)
                     /gene="emrR"
                     /locus_tag="ACHKUC_003623"
     CDS             complement(3278..3808)
                     /gene="emrR"
                     /locus_tag="ACHKUC_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO6619809.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(3899..4234)
                     /gene="ygaH"
                     /locus_tag="ACHKUC_003624"
     CDS             complement(3899..4234)
                     /gene="ygaH"
                     /locus_tag="ACHKUC_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO6619810.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(4224..4961)
                     /gene="ygaZ"
                     /locus_tag="ACHKUC_003625"
     CDS             complement(4224..4961)
                     /gene="ygaZ"
                     /locus_tag="ACHKUC_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO6619811.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGFLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(5085..6269)
                     /locus_tag="ACHKUC_003626"
     CDS             complement(5085..6269)
                     /locus_tag="ACHKUC_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6619812.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(6461..7453)
                     /gene="proX"
                     /locus_tag="ACHKUC_003627"
     CDS             complement(6461..7453)
                     /gene="proX"
                     /locus_tag="ACHKUC_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO6619813.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(7510..8574)
                     /gene="proW"
                     /locus_tag="ACHKUC_003628"
     CDS             complement(7510..8574)
                     /gene="proW"
                     /locus_tag="ACHKUC_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO6619814.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(8567..>9711)
                     /gene="proV"
                     /locus_tag="ACHKUC_003629"
     CDS             complement(8567..>9711)
                     /gene="proV"
                     /locus_tag="ACHKUC_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO6619815.1"
                     /translation="AFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSG
                     SGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMT
                     VLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALA
                     INPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIM
                     QNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGP
                     RSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQT
                     PLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         2392 a         2594 c         2525 g         2200 t
ORIGIN      
        1 tcagatatcc cataaagcct gatacgctca ttttgggcgg gatctccaca agcatatgga
       61 tatgatctgc acagcattca gcttccagaa tccgtacact tttccactca cacagctttc
      121 tcaaaataca gcctattgct ctacgcttct ctctgtagaa cacctgtctt cggtattttg
      181 gcgcaaatac tatgtgatat ttacagttcc atcgggtgtg cgctaagctc ttttcgttcc
      241 ccattgggac ccccttttga tttcttgttt gacacttgca gttgccagac cgcaaggtgt
      301 tttaacaaat caaaaggggt tttaataact ggctcaaagc tgaaagcttt ccggaacccc
      361 cagcctagct gggggttttc tgtgcacaaa aaagccagtt caaatgaact ggcttagttg
      421 tacttagtgc gcaccacctc cgccgccacc tgcgccaaat ggcggtttag caaaccacac
      481 cagccccagc aggacgagga atatcccggc tgacatccag aagatctcat tggcggaaat
      541 aatcagcccc tgattggtta tctgctgggc aatccagcct gatgcctgct gttgcgtcat
      601 accaagccct tccagttgac tgtacatcgc ctgagcattc gggttgaacg ggtttaccga
      661 ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc cacatggtcg tggttatcga
      721 cgtgccgata gaccccgcca gcgttcgcgt aaagttggag aggcttgatg ccgccgccag
      781 tcgttccggt ggcaaaccgg acagcgtaat ggtggtcagc ggcataaaga agcaggccac
      841 cgcaaaaccc tggataaact gtggccaggc cgacgcgcca aaatccatac ccggttcaaa
      901 ggtataggca cgccagtaga agcagacggc atacataata aagctgaagg ttaccagtcg
      961 gcgcatatcc agtttatgcg caaagcggcc gataatcggc gacaggatca ccggaataat
     1021 ccctaccggc gcagaggcca gccctgccca cgtcgccgtg taaccgtaga cctcctgcaa
     1081 caactgcggc agcagaacaa tcgcgccgaa gtagagcata tacgcgaggc tgatacacaa
     1141 gcagccgatg gtgaagttac gcgacttaaa caacgacaga tcgactatcg ggttatcgtc
     1201 ggtcagctcc cagacaatca ggaagcagat agccaccacc gccaccacgg tgaggatgat
     1261 aatttcctgt gatgagaacc agtccagctc tttaccgcgg tcgagcataa tctgcaggct
     1321 gccgatacca ataaccagca gtgccagccc cacggcatca atccgccgcc gttcggtgcg
     1381 ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc accgccacgc caatcggcac
     1441 gttgatgaag aatatccagc cccagtggta attatcactg atataaccgc cgaggatcgg
     1501 gccgcaaatt ggcgcaacaa tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt
     1561 ggctggcggg tagttattca gcaatagact ttgcgaaagc gggatcagcg gcccggcgac
     1621 aatcccctga atcacgcgga agaagatcag catattcagg ctgctggaga caccacacgc
     1681 ccacgacgca atagcaaagg cgatggttga ccagaggaac aatttcactt ccccgatgcg
     1741 ctttgccagc cagccggtaa gcgggatcga gatggcattc gccaccccga aagaagtgat
     1801 cacccacgtt ccctggctga gcgatgagcc cagattcccg gcgatagtgg ggatcgccac
     1861 gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat
     1921 aatgaccagt tgtgcgcctt ccagcggttt ttgctgttgc atcacacgca cctctggatt
     1981 agccagcgtt agcttttacg atatcgtcga tcagtttatt gacaggtgcc aggctgattt
     2041 cacgcgcggt gcttaccgcg accggtgtgg aacgtacttt atttgccagt acctgaccgt
     2101 cacggttagt ggtattgaca ctcaccagcg tggataaacc gatacgcagc gggtattgct
     2161 ccagctgttt ctggtccagt tcaatacgca caggcagacg ctgaacgact ttgatccagt
     2221 taccggtggc gttttgcgcc ggaagcagtg agaacgcgct acctgtgccc atatccagac
     2281 caaccacttt accggtgtat ttcacatcat cgccgtaaat atcggtggtg atggtgaccg
     2341 gctgaccgat acgcatattg gcaatctgcg tctctttaaa gttggcatcc acccacatat
     2401 tggtggctgg aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc ccaggctgta
     2461 ccgcgcggcg ggagacataa ccggtcatcg gactgacaat acgggtacgc tccagcgcca
     2521 gccaggcgtt acgtacttcg gtggcagcct gttgtacggc aggctgatct tccagtttcg
     2581 tccccagaat catcgcctga ttggcattgt attgttgaat agcgacgtcc agttgcgcct
     2641 gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat
     2701 tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt cgcgagggcg attttttgca
     2761 cctcaatatt cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc
     2821 tggaagccag tgcagttttg gctttttcaa atgcctggcg agcatctgtc gggtcgagag
     2881 tgaccagcac gtcgccttct tttacaaaat cggtgttatc ggcccagact ttcgtcacgc
     2941 tgccagacac ctgagacata atttgaatct gattccctgc cacgtatgcg tcatcggttt
     3001 cttcgaaatg acgcagtacc aaaaaccaat aaatccctat cgctacggca ataattataa
     3061 agagcaaggt gagaaggagg agcagacgct tacgtttgcc gctcttcttt accggttgct
     3121 gcggggtttg agtctccgca tttgcgctca tattgttctc cacgatcttc ttaactcatc
     3181 ggctgagccg acctgcttgc caaaaaggcc agcatgttac gatgctggcc ggttattttt
     3241 tcttttataa atctggattt ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca
     3301 caccgtcctg ttccatctgg tcgagacggg agagcaattt gcgggtgatt tgctcgagct
     3361 ggtctttttc tgttgtgctg agcgcggacc agagttgatg caggcagtta tgctgcggcg
     3421 gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc agacagcggc
     3481 gatcgttatc gctttcacga cgttcgatcc aaccgcgttt ttccagttca tcggcaatac
     3541 gcgtcgcgtt ggtacgggat gatccaagag cacaactcaa ttcagaaggc tgaatactgt
     3601 ggttttcctg agactccagc gtaatcaacg ccataaacaa cgtctcgtta atcccctgag
     3661 ccttcagcat tttattgcgg ttttccagca gcttgctttg catatgcatg caaagacggg
     3721 tcagaaggat ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt
     3781 gttcaatggg cgtaaacgaa ctatccattt gggtatgacc tcattaattt cagccgatat
     3841 agtaacgaca gtgacaaata aagtaaatgt attgttttag aaaaatgatt cttgtgggtt
     3901 atataatcgc catcactttc caggcgagcc cataggccag cgcactaagc agtgttggga
     3961 tgataatgct gcgtgtttta tagaaactgg cacccagtac cgcgaagccg accagtgtgg
     4021 gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc agcagagcgc
     4081 atatcgaggc gatgccaatg gtgtcgagca aaatacctac cgcgccacgt ttggttgggc
     4141 gggcattacc cacacgcagg cgcagcggca gatagcggaa gcaataattc gccgcgccaa
     4201 ccagtaaccc aagcagcaga acctcatagc tcatcgggcg ctccttgcca gaatgcctgg
     4261 attaacgcag tgaggcagcc acagacaatg cctgccagaa tggcgacggg aatagaaaat
     4321 agcgttacgc ctgcaagggc accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc
     4381 tggaaagagg cgagcaggaa actcataaag agtgccggaa gcataaaacc taatgctgct
     4441 tcaacggcgg gataaccttg cagaaagccg ctgccggaga atgcccctat taccgtacca
     4501 aatacccacg atgaccatga actgaaggca atgccgatca tccagttttc gctccagcgg
     4561 cgattattgc gtaccagttt tgcggttgcg gcggcaaaaa cctcatccgt caggccaaac
     4621 gcccacaggg cggttttcga tttttgcaga cgctgaataa tacggctacg cagtgacggg
     4681 ccatacaaaa catggcgaac atccattgcc atgacggtca gtgcagcaac ccacaaacta
     4741 ctcccggctg ccagcatcgc ggtaatgacg aactggctcg cgcctgcata aatgatgcag
     4801 gagaaaaaaa cgctttcgag aggagagaat cccagacggg tcgcattcag accgaacgca
     4861 aaggccaccg gaatataact aataacaatc ggtaaactgt ctttgcatcc ttccatgaag
     4921 gtcgccgaac caggagcagg ctgtggagta gggctttcca tagagtgtac gcttaacgat
     4981 tgttaatatt ataaatagac tgaatgaata tcttaacctt atcagactga tgggcttctt
     5041 aacaccctta taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt tgcggcttcc
     5101 tgacgatgaa aacctcgcca ccagaccagt aagttaacca gggcaatcgt cgcgccagcc
     5161 agacacacgc cagcccaacc gccatgttgc caggctgagg ctgaaattag cgaaccggcg
     5221 gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg
     5281 tgtattcgat aaattaccgt ctggttagtg atatgcacgc cctgcacggt gaggtccagc
     5341 accaggatgc cgataatcag cgccagtacg gaagtgtgac caaaccagat tgccagccat
     5401 gaaagtaaca gcagcagcag accgaaagtt gtggtgtggt gcgatttgcc cttatcggca
     5461 aaaccgcccg ccggacgagc gcccaacgct ccggcagctc ccgcaagccc aaacagacca
     5521 attacaccat cgctgtagtt aaaaggtgga gcggcaagca aaaaggccat tgaggtccag
     5581 agaatgctga agttggcaaa ggtcaggcag cccagcaacg cgcgggtgcg caggatctta
     5641 ttgctgataa acatgctgaa aacggaaccc aacaactgtg ggtagttgag gtgggtttct
     5701 gatttcattt gtggcagacc acgccataat gccagcgcca tcagtgccat taacatcgaa
     5761 gcaacccaaa agacggtgcg ccagccgccg agattcgcca gcaatccggc aacggtccgt
     5821 gcgagtaaga tccccaatag cagaccgctc ataatggtgc caaccacttt gccgcgtttg
     5881 tccggtgaag ccagtgtcgc tgccagtgga accagaattt gtgccacgac tgaaaataaa
     5941 ccggttaatg ctgtaccgag gatcatcatc gccagcgact gactgctggc agtgattaac
     6001 atgccgcctg ctgccagtaa ggtcatcgag acaatcaggc ggcggcgttc aaacatatca
     6061 ccgaggggaa caagaaacag cagacctgcg gcatagccca actgtgcggc ggtaacaata
     6121 aagcctgccg aactggcgga aagggaaaag ttacgcgcga tggtgtcgag caatggctgg
     6181 gcgtaatagt tactggctac cgccagaccg gtggcgatag acatcagcac gatcagcgcc
     6241 gggctaagct catgattagg tttagtcatt atttttgttc atcaggtgat gattaagctg
     6301 gaaatcaata atagcggatg ttgatgaata tgggatgaaa gatagattgt tgaaatgtgc
     6361 ggtgtggttt gtaggtatga taagacgcgc cagcgtcgca tcaggcattg tgtacagagc
     6421 ggcggatgag gcaaaaacgc cttatccgcc cgaataaaaa ttacttctgc gctgccagcg
     6481 cttcattcac ccagccatcg aactgctgct ggtgggcttt gatccaacca tcaacgtggc
     6541 cctgaatatc gtcttctgag gctttgccgt catgcatgat ggcgttctgg gcgttaatat
     6601 cagccactgg taactgcata atggcaaaca gtttcgctgc tgccgggttt ttctcggccc
     6661 aggctttgtt ggcaacgata tgcatggtac tgaccgggaa gccgtaattc gcgccattcg
     6721 gcagtttggt atcggcattt ttatcgcccg gcagtgcgga gaacggcacc tgcaaccaga
     6781 ccacatcttt ccccggtttc agttcgttac tcacccagta cggcgtccag gtgtagtaaa
     6841 acaccggctt accctctttg taacgactga tggtgtcagc catcatcgct gcgtagttcc
     6901 cctgattatg cgtcacggta ttggtcagtc catacgcggc aagctggtgg ttgatcgcac
     6961 cttcgcagcc ccagcctggg ttacagccgg ttaaatccgc tttaccgtcg ccgttggtat
     7021 cgaacagttt ggcgatcttc ggatctttaa gttgcgcgat gttggtaatt ttatactggt
     7081 cggcagtttt cttatcgatc aggtaaccct gtgccgcgcc gttaacaaat actccttcac
     7141 gataaaattt cttatcgcca ccggcagctt cgtacatgtt gtcatgcagt ggcgtccagt
     7201 tcactgcggt gaaggttgca tcgccggaag caagcgaggt gtagccaacg ttgtaatcca
     7261 cttcgctggg tttattgacg gtataaccta atttctccag cgcacgactt accagcagtg
     7321 tctggaaggt ttcttcggtg atggtgcttt gaactggatt aacagtaatg cctttgcccg
     7381 gcaggtcggc agcaaaagtt tgtgtagaga taagcgtggc aaacgctgtc gcaaaaagta
     7441 cgctatgtcg cattgttatt cctttttttg gcagggtgat atgcccggcg tcaccgggca
     7501 agtgcagagt tacttaatga atgggcgggt cagcagaccg acagggccag tggtgtacca
     7561 gcgacgattg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat
     7621 aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat ccagacgacc
     7681 gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg cgatgaccac
     7741 catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggttg gcatcgccag
     7801 cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc gcgaggcttc
     7861 aatcagatcc gccggaacct ggttaattcc cagaatcgtc agacggataa tcggcggcag
     7921 cgcaaagatg atcgtcacca ccacgcctgg cacgttgccg ataccaaaca gcatgacgat
     7981 tggcaccaga taaacaaacg cgggcgtggt ctgcatggca tcaagcagtg gacgaataat
     8041 tttcgccgct cgcggacttc tcgccagcca gatccccaac ggcaaaccta tgacgataca
     8101 gaacagcagg gctgttaaca ccagcgccag ggtaaccatt gcctgtgacc aggcaccgat
     8161 tgcgccgatg gcaattagcg aaaccagcgt cgccacgccc attccgaccc cggaaatctg
     8221 ccaggcgatg agagcgaaaa cgataatcgc caccggcgcg ggcataccca gcagcaattg
     8281 ctggaaaccg ttgaggatgt aatcaaccgg aacgcgcacg ccctgaaaga cgggacgaaa
     8341 atgggtaacg acccagtcga tcccttcagt gacccaactg tcgagcggga tcagcgtttt
     8401 atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac tggtcagcca
     8461 gtcagcgcca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt ctgcggattg
     8521 tgcagcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag ccattattta
     8581 ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg acatactgtt
     8641 ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag agcaattcgc
     8701 taagaggcgt ttgtgcatcg accgctaacg gcgcatcaat cagcgccgca tcaagacctt
     8761 gctgctgcgt taacgcggct ttaagcgaat cgatagacac agcgccgaca aacttattac
     8821 cgcgttcgat gacgtagcca tattcgcgat cttcatcctg caataatttc agtgccgaac
     8881 gcgggccgaa gccgggggtt ttacgaatta agccgttcgg tgtacgacgg gcaatatctt
     8941 tcgcactgaa cacctggcta atatcgacgc cacggaagaa ggttcggaca taatcattcg
     9001 ccggattatt aagaatttca tccggtgtac caacctgcac cacttcacca ttttgcataa
     9061 tggcaattcg gtcaccaata cgcatagctt catcgagatc gtgggaaata aagacaatgg
     9121 tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg cgaattaatg
     9181 gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc gctaacgcgc
     9241 gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag ctgtgggcat
     9301 aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt tcttcggcat
     9361 taattccggc caattccata ccgaatgcag tattgtccag cacggtcata tgcggcatta
     9421 aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg agttcggcgt
     9481 cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg ggttcaatca
     9541 ggcgattgag aaggcgtacc attgtggatt tacccgaacc ggataatccc atgatgacaa
     9601 atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat agcccagttt
     9661 tttccagaat ttgttctttt gaaagtcctt gttcgatata tttgaaagct c
//
LOCUS       DAZFRG010000183         9699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFRG010000183 DAZFRG010000000
VERSION     DAZFRG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.184"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..279
                     /locus_tag="ACHKUC_003630"
     CDS             <1..279
                     /locus_tag="ACHKUC_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO6619816.1"
                     /translation="VSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISES
                     GEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            279..5549
                     /gene="traI"
                     /locus_tag="ACHKUC_003631"
     CDS             279..5549
                     /gene="traI"
                     /locus_tag="ACHKUC_003631"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO6619817.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEGASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKDTGFDIRAYRDAADQRAETRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDVMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSIIRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVVSGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTCRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRRAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5569..6315
                     /gene="traX"
                     /locus_tag="ACHKUC_003632"
     CDS             5569..6315
                     /gene="traX"
                     /locus_tag="ACHKUC_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO6619818.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6370..6930
                     /locus_tag="ACHKUC_003633"
     CDS             6370..6930
                     /locus_tag="ACHKUC_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO6619819.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVIDEK"
     gene            7065..7277
                     /locus_tag="ACHKUC_003634"
     CDS             7065..7277
                     /locus_tag="ACHKUC_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6619820.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCRRRCRGRV"
     gene            7522..7983
                     /locus_tag="ACHKUC_003635"
     CDS             7522..7983
                     /locus_tag="ACHKUC_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6619821.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVIRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVTGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8029..8238
                     /gene="hha"
                     /locus_tag="ACHKUC_003636"
     CDS             8029..8238
                     /gene="hha"
                     /locus_tag="ACHKUC_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6619822.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     EHRLAELITGKLYDRIPKEIWKYVR"
     gene            8276..8962
                     /locus_tag="ACHKUC_003637"
     CDS             8276..8962
                     /locus_tag="ACHKUC_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO6619823.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLIRIVDMKRWYLCPQVRGADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSYDARKLLQMTGEWLDTFRTDLQSEPEGSRGENVHRFTRQQQASETAVWKV
                     KSDSCTEGNK"
     gene            8959..9267
                     /locus_tag="ACHKUC_003638"
     CDS             8959..9267
                     /locus_tag="ACHKUC_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619824.1"
                     /translation="MIVPVDAERDERGYWTHPALFGSGCESAADFHYWLRTQGLQCFV
                     MTMWDEVPELFAVRSGGEEPDARDWMPEPPDDDGWFLGSVHDTRYGPACYWLRHIRTG
                     "
     gene            9546..9695
                     /locus_tag="ACHKUC_003639"
     CDS             9546..9695
                     /locus_tag="ACHKUC_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6619825.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVMQVTLA
                     YEARK"
BASE COUNT         2301 a         2331 c         3109 g         1958 t
ORIGIN      
        1 gtgtctcctg ccatcaacga taagaagtca gattcaggtg tgaatgttcc ggcagggggg
       61 atcgagcagg agctgaaaat gaaaccggaa gaagagatgg aacagcaact gccacccggg
      121 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca acaggaaaat
      181 catccggaca tccagcagca gatgcagcgt cgtgaagagg tgaacattaa tgtgcaccgg
      241 gagcgcgggg aggatgttga gccgggagat gatttctgat gttgagtttc agcgttgtta
      301 aatcggcagg cagtgccgga aactattaca ccgacaagga taattactat gtgctgggta
      361 gcatgggaga acgctgggcc ggtcaggggg ctgaacagct tgggctgcag ggcagcgtcg
      421 ataaggatgt ttttacccgt cttctggagg gcaggctgcc ggacggggcg gatttaagcc
      481 gcatgcagga tggcagtaat aagcatcgtc ccggctacga cctgaccttc tccgccccca
      541 aaagtgtctc catgatggcc atgctcgggg gggataaacg tcttattgat gcacataacc
      601 aggctgtgga ttttgctgtc cgtcaggtgg aggcgctggc ctccacacgg gtgatgacgg
      661 acggacagtc agaaacggtg ctgaccggta atctggtgat ggcactgttt aaccatgaca
      721 ccagtcgcga tcaggaccca cagttacaca cgcatgtggt ggtggctaat gtcacgcagc
      781 ataacggcga gtggaagaca ctgagcagtg acaaagtggg gaaaaccgga ttttccgaga
      841 acgtgctcgc gaaccggatt gcgttcggga aaatatatca gagtgagtta cgtcagcgcg
      901 ttgaggcgct gggttatgaa accgaagtgg tcggtaagca cggtatgtgg gagatgccgg
      961 gcgtaccggt ggaggccttt tccggacgct cacaggctat cagggaggcc gtcggggaag
     1021 gggcctcgct gaaatcccgg gatgtggcgg cactggatac gcgtaaatcc aaacagcacg
     1081 tcgatccgga agtcagaatg gctgaatgga tgcagacgct gaaggacacc gggttcgaca
     1141 tccgggcgta tcgtgacgcg gcggatcaac gggcggagac ccgcacgcag gcgcccgggc
     1201 cagcttcaca ggacgggccg gatgtgcagc aggcggtgac acaggcgatt gccggattaa
     1261 gtgaacgaaa agtgcagttc acgtatacgg acgtgctggc caggacggtc ggcatactgc
     1321 cgccggaaaa tggtgtgatt gaacgggcac gcgccggtat cgatgaggcc atcagccgtg
     1381 agcagcttat ccccctcgac cgtgagaagg ggctgttcac atcaggaatt catgtgctcg
     1441 atgagctgtc cgtccgggca ctcagtcgtg acatcatgaa acagaaccgg gtgaccgtac
     1501 atccggagaa aagtgtcccc cggacggccg gttacagcga tgccgtcagt gttctggcac
     1561 aggatcgccc gtcgctggcc attgtgtccg ggcagggcgg tgcagccggg cagcgtgagc
     1621 gggtggctga actggcaatg atggcccggg agcaggggcg ggaggtgcag attatcgctg
     1681 ctgaccgtcg ttcgcagatg aacctgaagc aggatgaacg gctgtccggt gagctgataa
     1741 ccggacgtcg tcagctgctg gagggtatgg ccttcacacc gggcagtacc gttatcgtcg
     1801 accagggcga aaaactctcc ctgaaagaga cgttaaccct gctggacggt gccgcacgtc
     1861 ataacgtaca ggtcctgata accgacagcg ggcagcgaac cggtacaggc agtgcgctga
     1921 tggccatgaa ggatgccggg gtgaacacgt atcgctggca gggcggagaa cagcgaccgg
     1981 ccaccatcat cagtgaaccg gaccggaatg tccgctatga ccggctggcc ggagattttg
     2041 cggccagcgt gaaagccgga gaagagagcg tggcacaggt cagcggggta cgggaacagg
     2101 ccatactgac acaggccatt cgcagtgagc tgaaaacaca gggcgtgctc ggacacccgg
     2161 aggtgaccat gaccgccctg tcaccggtct ggctggacag ccggagccgt tatctgcggg
     2221 atatgtaccg accggggatg gtgatggagc agtggaaccc ggagacacgc agtcatgacc
     2281 gctatgtgat agaccgggtg acagcgcaga gtcacagcct gaccctgcgg gatgcgcagg
     2341 gcgaaacgca ggtggtgcgt atttcctccc tggacagcag ctggtcgctg ttccggccgg
     2401 aaaaaatgcc ggtggcagac ggcgagcgtc tgagggtgac agggaaaatt cccggactcc
     2461 gcgtctccgg cggtgaccgc ctgcaggtgg catccgtcag tgaagatgtg atgacggttg
     2521 ttgtgccggg gcgggctgaa ccggccaccc tgcctgtggc tgattcaccg ttcacggcac
     2581 tgaagctgga gaacggctgg gtggaaacgc ccgggcattc cgtcagcgac agtgcgacgg
     2641 tttttgcctc cgtcacacag atggcgatgg acaacgccac cctgaacggt ctggcccgca
     2701 gcggtcgtga tgtccggctg tattcctcac tggatgaaac ccgtactgcg gaaaaacttg
     2761 cccgccatcc ctcctttacg gtggtttctg agcagataaa ggcgcgtgcc ggtgagacat
     2821 tgctggaaac cgctatcagt ctgcagaaag ccgggcttca cacgccggca cagcaggcca
     2881 ttcatctggc ccttcctgtg ctggaaagta aaaacctggc cttcagcatg gtggacctgc
     2941 tgacagaggc gaagtcgttt gctgcagaag gaaccggttt tactgaactg ggaggggaaa
     3001 tcaatgcgca gataaaacgc ggtgacttac tgtatgtgga tgtggcaaaa ggctatggca
     3061 caggtctgct ggtttcccgt gcgtcgtatg aggcagaaaa gagcattatt cgccatattc
     3121 tcgaaggtaa ggaggcggtc acgccgctga tggagagagt acccggcgaa ctcatggaga
     3181 cgttaacgtc gggacagcgt gccgccaccc gtatgatact ggaaacgtcc gaccgtttca
     3241 cggtggtaca gggttatgcc ggtgtgggta agaccacaca gttccgggcg gtgatgtcag
     3301 ccgtgaacat gctgccggcg agtgagcgtc cccgagttgt ggggctgggg cccacgcacc
     3361 gtgcggtcgg tgagatgcgc agcgccggcg tggatgcaca gacgctggcg tcctttctgc
     3421 atgacacgca gctgcagcag cgcagcggag aaacgccgga tttcagcaac acgctgttcc
     3481 tgctcgatga gagctcaatg gtgggcaata ccgacatggc acgggcatac gccctgattg
     3541 cggccggtgg cggtcgtgcc gtggtcagcg gtgacacgga ccagctgcag gccatcgcgc
     3601 ccggtcagcc tttccgtctc cagcagacgc gcagtgctgc cgatgtggcc atcatgaagg
     3661 agattgtgcg tcagacgccg gaactgcggg aggcggtata cagcctgatt aaccgggatg
     3721 tggaaagggc actgtccggg cttgagagtg tgaaaccgtc tcaggttccc cgtcaggagg
     3781 gcgcatgggc accggagcac tccgtgacgg agttcagtca cagccaggaa gcgaaactgg
     3841 cagaagcgca gcagaaggcg atgctgaaag gcgaggcttt cccggatgtc cccatgacac
     3901 tgtatgaagc cattgtccgc gactatacct gcaggacacc ggaagcacgg gagcagacgc
     3961 tgattgtcac gcacctgaat gaggaccggc gcgtactgaa cagcatgatt catgatgcac
     4021 gggaaaaggc cggtgagctg gggcaggagc aggtcatggt gcctgtcctg aacacagcga
     4081 atatacgtga cggggagctg cgtcgtctct ccacctggga gaataacccg gatgcccttg
     4141 ccctggtgga taatgtgtat caccggattg ccggtatcag cagggatgac gggctgataa
     4201 ccctgcagga tgctgaaggt aacacgcggc tgatttcgcc ccgggaggcg gtggctgaag
     4261 gcgtcacact gtacaccccg gacaccatcc gggtggggac cggtgaccgg atgcgcttca
     4321 cgaagagtga ccgggagcgc ggttatgtgg ccaacagcgt ctggacggtg acagcggttt
     4381 ccggtgacag tgtcacgctg tcggacggac agcagacccg ggtgattcgc cccggacagg
     4441 agcgggcaga gcaacatatt gacctggcct atgccatcac cgcccacggt gcgcaggggg
     4501 caagtgaaac ctttgccatc gcgcttgaag gcacggaagg taaccggaaa ctgatggccg
     4561 gctttgagtc agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag gtgtacaccg
     4621 ataaccgtca gggctggacg gatgccatta acaatgccgt acagaaagga actgcccacg
     4681 atgtgtttga gccgaaaccg gaccgggagg tcatgaatgc gcagcggctg ttcagtacgg
     4741 cacgggaact gcgggacgtg gcggcaggcc gtgccgttct ccgtcgggca gggctggccg
     4801 ggggagacag ccctgcacgg tttattgcac cgggacgtaa atatccgcag ccgtatgtgg
     4861 cactgccggc gtttgaccgt aacggcaggt ccgccggtat ctggctgaac ccactgacca
     4921 cggatgacgg aaacgggctg cggggattca gtggtgaagg acgggtgaaa ggcagcgggg
     4981 atgcgcagtt cgtggccctg cagggcagcc gtaacggaga gagcctgctg gctgataata
     5041 tgcaggatgg cgtccggatt gcccgtgata atcctgacag cggtgtggtg gtgagaattg
     5101 ccggtgaagg tcgtccgtgg aatcccggtg ccatcaccgg tggccgcgtg tggggggata
     5161 tcccggataa cagcgtccag ccgggggccg gaaatggcga accggtcacg gcagaggtac
     5221 tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgc gcagatgaaa
     5281 ttgtcaggaa aatggcagag aacaaacctg acctgccgga cggcaaaaca gagctggctg
     5341 tcagggatat tgccggacag gagcgtgacc ggtctgccat atctgaacgg gaaaccgcac
     5401 tgccggagag tgtgctgcgt gaatcacaac gggagcggga agcggtccgg gaggttgccc
     5461 gggaaaatct gctgcaggag cgtctgcagc agatggagcg ggatatggtt cgtgacctgc
     5521 agaaagagaa aaccctgggc ggagactgat acaggaagac aaacactgat gacaacggat
     5581 aacacgaaca cgacacgtaa cgattcactg gctgcacgga ccgatacctg gttgcagtca
     5641 tttctggtct ggtcacccgg acagcgggat atcatcaaaa cggtggcact ggtgctgatg
     5701 gtactggacc acatcaatct gatattccag ctgaagcagg aatggatgtt tctggccggg
     5761 cgaggagcct ttccgctgtt tgccctggtg tgggggctga atctgtcccg tcatgcgcat
     5821 atccggcaac cggccattaa ccggctgtgg ggatggggaa ttattgccca gttcgcatat
     5881 tatctggccg gctttccctg gtatgagggg aatatcctgt ttgcctttgc agtggcggca
     5941 caggtgctga cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc cattcttctg
     6001 atggccctgt gggggccttt gtccggcacc agttacggca ttgccgggct gctgatgctg
     6061 gcggtcagtt accgactgta ccgggcggaa gacagagcgg aacgtctggc gctgcttgca
     6121 tgcctgctgg ccgttattcc ggcacttaac cttgcctcca gtgatgcagc ggcggtggcc
     6181 ggtcttgtaa tgacggtgct gaccgttggt ctggtgtcgt gtgcaggaaa atcattaccc
     6241 cgtttctggc ccggggattt tttcccggta ttctacgcct gtcatctggc tgtgctgggc
     6301 gttctggcgc tgtgacgggt gtggtatctt tgaccgtaac aggatgattc gtcagaggca
     6361 gaacacagca tgacagagca gaagcgaccg gtactgacac tgaagcggaa gacagaggga
     6421 acagcgcctg tccgcagccg gaaaaccatc atcaatgtca ccacgccacc aaaatggaag
     6481 gtgaaaaagc agaagctggc ggagaaagca gcccgggaag cagagctggc ggcaaaaaaa
     6541 gcgcaggcca gacaggcact gtccatttat ctgaacctgc ccacgctgga tgaggccgtg
     6601 aataccctga aaccctggtg gccgggatta tttgacggtg acaccccccg gcttctggcc
     6661 tgcggtatcc gggacgtgtt actggaagac gtggcgcagc ggaatatccc gctctcgcat
     6721 aaaaaactgc gcagggcgct gaaggccatc acccgttcag aaagctatct gtgtgccatg
     6781 aaagccggtg cctgccggta tgacacggaa gggtatgtga cggagcatat ttctcaggag
     6841 gaggaagcgt atgcggcagc gcgtctggat aaaatccgcc gccagaaccg gataaaggca
     6901 gaacttcagg ccgtgattga tgagaaataa aaaggcctcc ccgaaagagt gaggaggcag
     6961 aaacaataag gcttaaaagg aaataacttc cgtacccgaa aggaagtcta tccagtggat
     7021 tatttaacac cataatacag acgggttaaa ggggagagct aaagatgaat ggattcagaa
     7081 acagttcacg gaacggtcag gtctggcgct accagcgtgc cggtgggcgg gctgttattc
     7141 tggaggtcag tggacgctgg atggaagcgg cagaagcatg gcgacgggct gcctgtatag
     7201 ctccccggac agactggcaa cagtttgccc ggaaaagggc tgaacactgc cgccggcgct
     7261 gccggggaag ggtgtaacga aaaaaacagc cttcattaca ccagaagtca gaaggctgaa
     7321 aggagtttat gagatttatt tttttaatat ccggtcgggc agtatgaccg gacagttctg
     7381 aatagcacag agttttttcc gtattaagtc gcgattttat attttgtgat taatttcaca
     7441 aaataagatg ttgttcagtg tgtgctgcaa tattcagggt gtctgaaata caggtgccat
     7501 ttatttgata tggagaataa catgaggaaa tatattccac tggtattatt tatcttttca
     7561 tggccggtat tgtctgcaga tattcatgga cgggttattc gtgtactgga cggtgatacc
     7621 atcgaggtaa tggactcacg gaaggcggtg cggatcaggc tggtaaatat tgatgcaccg
     7681 gaaaagaaac aggattatgg ccggtggtcg acggacatga tgaaatcact ggttacagga
     7741 aaaacggtta ccgtcacata ttttcagcga gatcgttatg gtcgtattct cgggcaggtt
     7801 tatgcaccag acggaatgaa tgttaatcag tttatggtcc gggccggggc tgcctgggta
     7861 tatgagcagt acaacacaga tcccgtgctg cctgtccttc agaacgaagc cagacaacaa
     7921 aaacgtgggc tctggtcaga tgctgatccg gttcccccct ggatatggag acaccgtaaa
     7981 taaagcgatg gcaatcttat cattcatctg ttctcagcgg gaataaaaat ggcaaaaaca
     8041 aaacaggaat ggctgtatca gttacggcga tgttcatcag tcaatacact ggaaaaaatc
     8101 attcataaaa acagagacag tttatcgacc agtgaacgtg aatcatttaa ttcagcagca
     8161 gagcaccggt tagcggaatt aatcaccgga aagttatatg acaggatacc aaaagagata
     8221 tggaaatatg taaggtaata attcagcata attaattacc aggagtagat aaacaatgaa
     8281 gctgattatt tttattctca ttgttctgat tattgcagcg ttacttatcc ggattattct
     8341 cagaagtgta aatcagcatt ctcctttgct gatgcaattg catgcggcag gtatacggac
     8401 cggtgatgca gagcgaatac tgtccagcgg tgaatactgg caacgtcaga agacgctgct
     8461 gacagaaagg gaagtcagtt ttatgaaagg actgatcaga attgtggata tgaaacggtg
     8521 gtatctgtgt ccgcaggtac ggggcgcgga tatcgtccag ctgaacggga atatccggcc
     8581 acgatcacgc cagtggtggc agttattcag gatggtgtct cagtggcatg ttgatgttgt
     8641 tatcgttgag cggcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca
     8701 tttacgacca gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg
     8761 tattccgttg ctcagaagct acgatgccag aaaactgctg cagatgaccg gagaatggct
     8821 ggataccttc agaacagatc tccagtcaga accggaaggc agcaggggcg aaaatgtgca
     8881 tcggtttacg cgtcaacagc aggcatcaga aacagcggta tggaaggtga aaagtgattc
     8941 ctgtacggag ggcaataaat gatagttccc gtcgatgcag agcgtgatga gcgggggtac
     9001 tggacgcacc ctgcactgtt cggcagtggc tgtgagagcg ctgcagactt tcattactgg
     9061 ctgagaacac aagggttgca gtgctttgtt atgactatgt gggatgaagt gccagagctt
     9121 tttgctgtca ggtctggcgg ggaggagccg gatgcgcgtg actggatgcc ggagccgcca
     9181 gatgatgatg gctggttcct gggatctgtc catgataccc gctacgggcc tgcctgttac
     9241 tggttacgtc atatcagaac gggctgacgc ctccgctccg cgtcgttgtc caaccccgga
     9301 aacgggaaaa agcaagtttt ccccgctccc ggcgtttttg caataaaaac cgggacgtgg
     9361 aggtaatagt cagtaacagg gaatgtggta ttatcgcggc gggtgtctga gcctttctgg
     9421 ttcaggcaag acgcaggtac cagaaatgcg aagaccccac ttgttaatcc attaactcgt
     9481 gaggcctgca tgatgtactt agcactactg attgtagcct cttccttgca gagaggtcaa
     9541 agtttatgac gaaatacacc cttatagggt tgctcgctgt gtgtgccacg gtgttgtgtt
     9601 tttcactgat attcagggag cggttatgtg agctgaatat tcacagggga aatacagtga
     9661 tgcaggtaac tctggcctac gaagcacgga agtaagctg
//
LOCUS       DAZFRG010000184         9597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFRG010000184 DAZFRG010000000
VERSION     DAZFRG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.185"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..1738
                     /gene="aceB"
                     /locus_tag="ACHKUC_003640"
     CDS             137..1738
                     /gene="aceB"
                     /locus_tag="ACHKUC_003640"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO6619826.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCAGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSHGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            1768..3072
                     /gene="aceA"
                     /locus_tag="ACHKUC_003641"
     CDS             1768..3072
                     /gene="aceA"
                     /locus_tag="ACHKUC_003641"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO6619827.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            3121..4845
                     /gene="aceK"
                     /locus_tag="ACHKUC_003642"
     CDS             3121..4845
                     /gene="aceK"
                     /locus_tag="ACHKUC_003642"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO6619828.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(4862..5686)
                     /gene="iclR"
                     /locus_tag="ACHKUC_003643"
     CDS             complement(4862..5686)
                     /gene="iclR"
                     /locus_tag="ACHKUC_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO6619829.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            5886..9569
                     /gene="metH"
                     /locus_tag="ACHKUC_003644"
     CDS             5886..9569
                     /gene="metH"
                     /locus_tag="ACHKUC_003644"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO6619830.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVVHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2291 a         2463 c         2690 g         2153 t
ORIGIN      
        1 tatttttaat taaaatggaa attgtttttg attttgcatt ttaaatgagt agtcttagtt
       61 gtgctgaacg aaaagcgcac aacgatcctt cgttcacagt ggggaagttt tcggatccat
      121 gacgaggagc tgcacgatga ctgaacaggc aacaacaatc gatgaactgg cttttacaag
      181 gccgtatggc gagcaggaga agcaaattct tactgccgaa gcggtagaat ttctgactga
      241 gctggtgacg cattttacgc cacaacgcaa taaacttctg gcagcgcgca ttcagcagca
      301 gcaggatatc gataacggaa cgttgcctga tttcatttcg gaaacagctt ccattcgtga
      361 tgctgactgg aaaattcgcg ggattcctgc ggacttagaa gatcgtcgcg tagagattac
      421 cggacctgta gagcgcaaga tggtgatcaa cgcgctcaac gccaatgtga aagtctttat
      481 ggccgatttc gaagattcac tggcaccaga ctggaacaaa gtgatcgatg ggcaaattaa
      541 cctgcgcgat gcggttaacg gcaccatcag ctatagcaat gaagcaggca aaatttatca
      601 gctcaagccc aatccagcgg ttttgatttg tcgggtacgc ggtctgcact tgccggaaaa
      661 acatgtcacc tggcgtggtg agacaatccc cggtagcctg tttgattttg cgctctattt
      721 cttccacaac tatcaggcac tgttggcaaa gggcagcggt ccctatttct atctgccgaa
      781 aacccagtcc tggcaggaag cggcctggtg gagcgaagtc ttcagctatg cagaagatcg
      841 ctttaatctg ccgcgcggca ccatcaaggc gacgttgctg attgaaacgc tgcccgccgt
      901 gttccagatg gatgaaatcc ttcacgcgct gcgtgaccat attgttggtc tgaactgcgg
      961 tcgttgggat tacatcttca gttatatcaa aacattgaaa aactatcccg atcgcgtcct
     1021 gccagacaga caggcagtga cgatggataa acccttcctg aatgcttact cacgcctgtt
     1081 gattaaaacc tgccataaac gcggtgcttt tgcgatgggc ggcatggcgg cgtttattcc
     1141 gagcaaagat gaagagcgca ataaccaggt gctcgacaaa gtaaaagcgg ataaatcgct
     1201 ggaagccaat aacggtcacg atggcacatg gatcgctcac ccgggccttg ctgatacggc
     1261 aatggcggta ttcaacgata ttctcggctc ccgcaaaaac cagcttgagg tgatgcgcga
     1321 acaagacgcg ccgattactg ccgatcagct gctggcacct tgtgctggtg aacgcaccga
     1381 agaaggtatg cgcgccaaca ttcgcgtggc tgtgcagtac atcgaagcgt ggatctctgg
     1441 caatggctgt gtgccgattt atggcctgat ggaagatgcg gcgacggctg aaatttcccg
     1501 tacctcaatc tggcagtgga tccatcatca aaaaacgttg agcaatggca aaccggtgac
     1561 taaagccttg ttccgccaga tgctgggcga agagatgaaa gtcattgcca gcgaactggg
     1621 cgaagaacgt ttctcccatg ggcgttttga cgatgccgca cgcttgatgg aacagatcac
     1681 cacttccgat gagttaattg atttcctgac cctgccaggc taccgcctgt tagcgtaaac
     1741 caccacataa ctatggagca tctgcacatg aaaacccgta cacaacaaat tgaagaatta
     1801 cagaaagagt ggactcaacc gcgttgggaa ggcattactc gcccgtacag cgcggaagat
     1861 gtggtgaaat tacgcggttc agtcaatccg gaatgcaccc tggcgcaact gggcgcggcg
     1921 aaaatgtggc gtctgctgca cggcgagtcg aaaaaaggct acatcaacag cctcggtgca
     1981 ctgactggtg gtcaggcgct gcaacaggcg aaagcgggta ttgaagcagt ctatctgtcg
     2041 ggatggcagg tagcggcgga cgctaacctg gcggccagca tgtatccgga tcagtcgctc
     2101 tatccggcaa actcggtgcc agcggtggtg gagcggatca acaatacttt ccgccgtgcg
     2161 gatcagatcc aatggtccgc gggcattgag ccgggcgatc cgcgctatgt cgattacttc
     2221 ctgccgatcg tcgccgatgc ggaggccggt tttggcggtg tactgaatgc ctttgaactg
     2281 atgaaagcga tgattgaagc cggtgcagcg gcagttcact tcgaagatca gctggcatca
     2341 gtgaagaaat gtggtcacat gggcggcaaa gttttggtgc caactcagga agctattcag
     2401 aaactggtcg cggcgcgtct ggcagctgat gtgacgggcg ttccaaccct gctggttgct
     2461 cgtaccgatg ccgatgcggc ggatctgatt acctccgatt gcgacccata tgacagcgaa
     2521 tttattactg gcgagcgtac cagtgaaggc ttcttccgta ctcatgcggg tattgagcaa
     2581 gcgatcagcc gtggcctggc gtatgcgcca tatgctgacc tggtatggtg tgaaacctcc
     2641 acgccggatc tggaactggc gcgtcgcttt gcacaagcca tccacgcgaa atatccgggc
     2701 aaactgctgg cttataactg ctcgccttcg ttcaactggc agaaaaacct cgacgacaaa
     2761 actattgcca gcttccagca gcagctgtcg gatatgggct acaagttcca gttcatcacc
     2821 ctggcaggta tccacagcat gtggttcaac atgtttgacc tggcaaacgc ctatgcccag
     2881 ggcgagggta tgaagcacta tgttgagaaa gtgcagcagc cggaatttgc cgccgcgaaa
     2941 gatggctata ccttcgtatc tcatcagcag gaagtgggta caggttactt cgataaagtg
     3001 acgaccatta ttcagggcgg cacgtcgtcg gttaccgcac tgactggctc cactgaagaa
     3061 tcgcagttct aacaaccaac gcccctttcc gtaaggcgag gggcgaatta aggagaggcg
     3121 atgccgcgtg gcctggaatt actgattgca caaaccattt tgcaaggttt cgacgctcag
     3181 tatggtcgat tcctcgaagt gacttccggg gcgcagcagc gtttcgagca agccgactgg
     3241 catgctgtcc agcaggcgat gaaaaaccgt attcatcttt acgatcatca cgtgggtctg
     3301 gtcgtggagc aactgcgctg cattactaac ggccaaagca cggacgcggc atttttactg
     3361 cgcgttaaag agcattacac ccggctgttg ccagattacc cgcgcttcga gattgcggag
     3421 agctttttca actccgtgta ctgtcggtta tttgaccacc gctcgcttac tcccgagcgg
     3481 ctttttatct ttagctccca gccagagcgc cgctttcgta ccattccacg cccactggcg
     3541 aaagactttc accccgatca cggctgggaa tctctgctga tgcgcgttat cagcgatctg
     3601 ccgctgcgtc tgcgttggca gaataaaagc cgtgacattc attacatcgt tcgccatctg
     3661 acggaaacgc tggggacaga caacctcgcg gaaagtcatt tacaggtggc gaacgaactg
     3721 ttttaccgca ataaggcagc ctggctggtg ggcaaactaa taacgccttc cggcacgttg
     3781 ccatttttat tgccgatcca ccagacggac gacggcgagt tatttattga tacctgcctg
     3841 acgacgaccg ccgaagcgag cattgttttt ggctttgcgc gttcttattt tatggtttat
     3901 gcaccgctgc ccgcagcgct ggtcgagtgg ctacgggaaa ttctgccagg taaaaccacc
     3961 gctgaattgt atatggctat cggctgccag aagcacgcca aaaccgaaag ctaccgcgaa
     4021 tatctcgttt atctacaggg ctgtaatgag cagttcattg aagcgccggg tattcgtgga
     4081 atggtgatgt tggtgtttac gttgccgggt tttgatcggg tatttaaagt catcaaagac
     4141 aggttcgcgc cgcagaaaga gatgtctgcc gctcacgttc gtgcctgcta tcaactggtg
     4201 aaagagcacg atcgcgtggg ccgaatggcg gacacccagg agtttgaaaa ctttgtgctg
     4261 gagaagcggc atatttcccc ggcattaatg gaattactgc tccaggaagc agcggaaaaa
     4321 atcaccgatc tcggcgaaca gattgtgatt cgccatcttt atattgagcg gcggatggtg
     4381 ccgctcaata tctggctgga acaagtggaa ggtcagcagc tgcgcgacgc tattgaagag
     4441 tacggcaacg ccattcgcca gcttgccgct gccaacatct tccctgggga tatgttattt
     4501 aaaaactttg gcgtcacccg tcacgggcgc gtggtgtttt atgattacga cgaaatttgc
     4561 tacatgacgg aagtgaattt ccgcgacatc ccgctgccgc gctacccgga agacgaactt
     4621 gccagcgaac cgtggtacag cgtctcgccg ggtgatgttt tcccggaaga gttccgtcac
     4681 tggttatgtg ccgacccgcg tattggtccg ctatttgaag agatgcacgc cgacttgttc
     4741 cgcgctgatt actggcgcgc actacaaaac cgcatacgtg aagggcatgt ggaagatgtt
     4801 tatgcgtatc ggcgcaggca gcggtttagc gtccgattcg tataaatcag aggcgaaaaa
     4861 gtcagcgtat tccaccgtac gccagcgtca cttccttcgc cgctttaatc accatcgcgc
     4921 caaactcggt cacgcggtca tcggtaatac gtgaaatcgg tccggaaatt gaaattgcgg
     4981 caaacggttc gcggtgctca tcgaaaatac acgctgcaag gcaacgcagc cccagcgcat
     5041 gttcctcatc gtcaaatgaa taaccccgtt tgcgcgtttg ggcgagatct tcttttaaat
     5101 gcacaggaga caccagcgtc gcgtgggtat aggcatgtaa ccctttgcgg tgcagcagct
     5161 tcgtcacctg ttcttcgctc agttgggcca gaaaggcttt acccgcaccg gaagcgtgca
     5221 tcggcaattt accgccgata ggcgcggaca ttcgcatcag gtgagtacac tgtacctggt
     5281 cgataataat cgcttcgtga ttgctttgat caagcaccgc catattgacc gtttcgccag
     5341 actcttccat tagattgcgc agaataggat ggacaatcgc taacaaatta cggctctgga
     5401 gaaagctgct accgaccata aaggcgtgtg cgccgattgc ccagtgaccc agttcgccga
     5461 cctgacggac aaagccctgt tgctgcatcg tggttagcag gcggtgggtc gtggaattgg
     5521 gtaacccggc ttgctgtgcc agttccgtga gtgccacact gccattggat tcggcaatcc
     5581 actccagtaa tttcaggcca cgcgttaaag actgaacctg tccagtcgct ggtgcggtgg
     5641 caacggcggg ttttctgccg cgttttgcgg gaatgggtgc gaccatgaca gtctcctttt
     5701 tctgtatcgt ggaaatcatt ttcattttta ttatcagcga atgcaatagt tgctgaacag
     5761 atccgatgag ttaatgttga acaaatctca tgttgcgtgg tggtcgcttt taccacagat
     5821 gcgtttatgc cagtatggtt tgttgaattt ttattaaatc tgggttgagc gtgtcgggag
     5881 caagtgtgag cagcaaagtg gaacaactgc gtgcgcagtt aaatgaacgt attctggtgc
     5941 tggacggcgg tatgggcacc atgatccaga gctatcgact gaacgaagcc gattttcgtg
     6001 gtgaacgctt tgccgactgg ccatgcgacc tcaaaggcaa taacgatctg ctggtgctca
     6061 gtaaaccgga agtgatcgcc gctatccaca acgcctactt tgaagcgggc gcggatatca
     6121 tcgaaaccaa caccttcaac tccacgacca ttgcgatggc ggattaccag atggaatccc
     6181 tgtcggcgga aatcaacttt gcagcggcga aactggcgcg cgcctgtgcc gacgagtgga
     6241 ctgcccgcac gccggagaaa ccgcgctacg ttgccggtgt tctcggcccg accaaccgca
     6301 cggcgtctat ttctccggac gtcaacgatc cggcattccg caacatcact tttgaccagc
     6361 tggtggcggc ttatcgagaa tccaccaaag cgctggtgga aggtggcgcg gacctgatcc
     6421 tgattgaaac cgttttcgac acccttaacg ccaaagcggc ggtctttgcg gtgaaaacgg
     6481 agtttgaagc gctgggcgtt gaactgccga ttatgatctc cggcaccatc accgacgcct
     6541 ccgggcgcac gctctccggg cagaccactg aagcgtttta caactccctg cgccacgccg
     6601 aagcgctgac ctttggcctg aactgtgcgc tggggccaga cgaactgcgc cagtacgtac
     6661 aggaactgtc gcgtattgcg gaatgctatg tcaccgccca cccgaacgcc gggctgccca
     6721 acgcctttgg tgaatacgat ctcgacgccg acacgatggc aaaacagata cgtgaatggg
     6781 cagaagcggg ctttctcaat atcgtcggcg gctgctgcgg caccacgcca caacatattg
     6841 cagcgatgag tcgtgcagta gaaggattag cgccgcgcaa actaccggaa attcccgtag
     6901 cctgccgttt gtccggcctg gagccgctga acattggcga agatagcctg tttgtgaacg
     6961 tgggtgaacg caccaacgtc accggttccg ctaagttcaa gcgactgatc aaagaagaga
     7021 aatacagcga ggcgctggat gtggcgcgtc agcaggtgga aaacggtgcg cagattatcg
     7081 atatcaacat ggatgaaggg atgctcgacg ccgaagcggc gatggtgcgt tttctcaatc
     7141 tgattgccgg tgaaccggat atcgctcgcg tgccgattat gatcgactcc tcaaaatggg
     7201 acgttattga aaaaggtctg aagtgtatcc agggcaaagg cattgttaac tctatctcga
     7261 tgaaagaggg tgtcgacgcc tttatccatc acgcgaaact gttgcgtcgc tacggtgcgg
     7321 cagtcgtggt aatggctttt gacgaacagg gccaggccga tacccgcgca cggaaaatcg
     7381 agatttgccg tcgggcgtac aaaatcctca ccgaagaggt tggcttcccg gctgaagata
     7441 tcatcttcga cccgaacatc ttcgcggtcg caactggcat tgaagagcac aacaattacg
     7501 cgcaggactt tatcggcgcg tgtgaagaca tcaaacgtga actgccgcac gcactgattt
     7561 ccggcggcgt ttctaacgtt tctttctcat tccgtggcaa cgatccggtg cgcgaagcca
     7621 ttcacgcggt gttcctctac tacgctattc gcaatggcat ggatatgggg atcgtcaacg
     7681 ccgggcaact ggcgatttac gacgacctgc ctaccgagct acgcgacgcg gtggaggatg
     7741 tgatcctcaa ccgtcgcgac gatggcaccg agcgtttact cgagcttgcc gagaaatatc
     7801 gcggcagcaa aaccgacgac accgctaaca cccagcaggc ggagtggcgc tcgtgggaag
     7861 tgaataaacg tctggaatac tcgctggtca aaggcattac cgagtttatc gagcaggata
     7921 ccgaagaagc ccgccagcag gctacgcgtc cgattgaagt gattgaaggc ccgttgatgg
     7981 acggcatgaa cgtggtcggc gacctgtttg gcgagggcaa aatgttcctg ccgcaggtgg
     8041 tgaaatcggc gcgcgtaatg aagcaggcgg tggcgtatct cgaaccgttt attgaagcca
     8101 gcaaagagca gggtaaaacc aacggcaaga tggtgatcgc caccgtgaaa ggcgatgtcc
     8161 acgacatcgg taaaaatatc gttggtgtgg tgctgcaatg taacaactac gaaattgtcg
     8221 atctcggcgt gatggtgcct gcggaaaaaa ttctccgtac cgctaaagaa gtgaatgctg
     8281 atttgattgg cctttcgggg ctgatcacgc cgtcgctgga cgagatggtt aacgtggcga
     8341 aagagatgga gcgtcagggc ttcaccattc cgctattgat tggcggcgcg accacctcaa
     8401 aagcgcacac ggcggtgaaa attgagcaga actacagcgg cccgacggtg tatgtgcaga
     8461 acgcttcacg caccgttggt gtggtggcgg cgttgctttc cgacactcag cgtgatgatt
     8521 tcgttgctcg tacccgcaag gagtacgaaa ccgtgcgtat tcagcacgga cgaaagaaac
     8581 cacgcacacc accggtcacg ctggaagcgg cgcgtgataa cgacttcgct tttgactggc
     8641 aggcttacac gccgccggtg gtgcaccgtc tcggtgtgca ggaagtcgaa gccagcatcg
     8701 aaacgctgcg taattacatc gactggacgc cgttctttat gacctggtcg ctggccggga
     8761 agtatccgcg cattctggaa gatgaagtgg taggtgttga ggcgcagcgg ctgtttaaag
     8821 acgccaacga catgctggat aaattaagcg ccgaaaaaat gctgaacccg cgtggtgtgg
     8881 tgggcctgtt cccggcaaac cgtgtgggcg atgacattga aatctaccgt gacgaaacgc
     8941 gtacccatgt gattaatgtc agccaccatc tacgccaaca gaccgaaaaa accggcttcg
     9001 ctaactactg tctcgctgac ttcgttgcgc cgaaggtttc cggcaaagcg gattacatcg
     9061 gcgcatttgc cgtgactggc gggctggaag aggacgcact ggctgatgcc tttgaagcac
     9121 agcatgatga ttacaacaaa atcatggtga aagcgcttgc cgaccgtctg gcggaagcct
     9181 ttgcggagta tctccatgag cgtgtgcgta aagtctactg gggctatgcg ccgaacgaga
     9241 acctcagcaa cgaagagctg atccgcgaaa actaccaggg catccgtccg gcaccgggct
     9301 accctgcctg cccggaacat acggaaaaag ccacaatctg ggagctgctg gaagtggaaa
     9361 aacataccgg tatgaaactc acggaatcct tcgccatgtg gccgggtgca tcggtttcgg
     9421 gttggtactt cagtcatcca gacagcaagt attacgcggt ggcgcaaatt cagcgcgatc
     9481 aggttgaaga ctatgcccgc cgtaaaggaa tgagcgttac cgaagttgag cgttggttgg
     9541 caccgaatct ggggtatgat gccgagtaag tgataacacc ggggtatttg ccccggt
//
LOCUS       DAZFRG010000185         9581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFRG010000185 DAZFRG010000000
VERSION     DAZFRG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.186"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..613)
                     /gene="mocA"
                     /locus_tag="ACHKUC_003645"
     CDS             complement(35..613)
                     /gene="mocA"
                     /locus_tag="ACHKUC_003645"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO6619831.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            717..1487
                     /gene="yqeC"
                     /locus_tag="ACHKUC_003646"
     CDS             717..1487
                     /gene="yqeC"
                     /locus_tag="ACHKUC_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO6619832.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEVIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            1535..3160
                     /gene="yqeB"
                     /locus_tag="ACHKUC_003647"
     CDS             1535..3160
                     /gene="yqeB"
                     /locus_tag="ACHKUC_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO6619833.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3201..4133)
                     /gene="arcC"
                     /locus_tag="ACHKUC_003648"
     CDS             complement(3201..4133)
                     /gene="arcC"
                     /locus_tag="ACHKUC_003648"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6619834.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINHAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4181..5566)
                     /gene="hyuA"
                     /locus_tag="ACHKUC_003649"
     CDS             complement(4181..5566)
                     /gene="hyuA"
                     /locus_tag="ACHKUC_003649"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO6619835.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5619..6830)
                     /gene="ygeY"
                     /locus_tag="ACHKUC_003650"
     CDS             complement(5619..6830)
                     /gene="ygeY"
                     /locus_tag="ACHKUC_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO6619836.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6888..8084)
                     /gene="dpaL"
                     /locus_tag="ACHKUC_003651"
     CDS             complement(6888..8084)
                     /gene="dpaL"
                     /locus_tag="ACHKUC_003651"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO6619837.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8142..9329)
                     /gene="ygeW"
                     /locus_tag="ACHKUC_003652"
     CDS             complement(8142..9329)
                     /gene="ygeW"
                     /locus_tag="ACHKUC_003652"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO6619838.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2243 a         2332 c         2487 g         2519 t
ORIGIN      
        1 tagatatttg aacgcaatcg tttaactacg ttttctaaat ttcagtatac cttttcttcg
       61 ctgtaataaa gtcgtccgga gtatcaatat ctaaaattat ttctgcattt tctatttcga
      121 cggaataatg ttctcccata aggagtgcct gacgcatatt ggtgacattg ggtcgctgga
      181 ttgcctgcat caggcatggt tttgacacta aaatcggatg gccggggatg ccattatgga
      241 gtggcagtat tgcgccatca tttcgtaacg accagatttt tctaaaaata tcgatggtga
      301 gggttggcat atctccgtgg gtgagaaaac aatgttctgt ttgcaccgcg ggtactgcgg
      361 cctttactga tgtcagtaaa ccctgcgcat aatctgggtt gtggataata gtgatattgc
      421 tctggttcgc ataacgttcg tgcagttcat taccacgata gccggtgact aaaataattc
      481 ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag aattgttccc tgttgccagg
      541 gtaacatcat tttccattgc cccattcttg atgataatcc tgcggcggta attatacagt
      601 cgatggctga catagtgatt ccattattta caggagctaa taacgcctga taattgcgtt
      661 acttcctgat ggattacgtt gagggtaatt tatcccagag aggtccataa agacgcgtga
      721 aaagtataat tgacccatcg gcattattca ttgatttagg cgcgcagaaa cgtcccactg
      781 tgatttctat tgttggtgct ggggggaaaa ccagcttgct tttttggctg gcagaactgt
      841 tccaggcgag tggcaggcgt gtattaatca ctacgacgac acatatgttt atgccaacat
      901 ctcactggcc cgtggttttc tgtcgtgatc ccgccatgct tcctcatgcg tcttttatat
      961 cccccatttc attttgtttt cacagctgga aagcgaacca gggaaaagtg cagggattta
     1021 cgccagaagt gattgatgca ctggtacaac gaccagagtg tgacgtaatt ctcattgagg
     1081 cagatggctc gcgtggaatg ccgttaaaag cgcctgatga gcacgaacct tgcataccta
     1141 aaagcagttg ctgcgtgatt gctgtgatgg gaggacatat tttgggtgcg aaagtgagca
     1201 cagaaaatgt ccatcgctgg tcgcagtttg ctgatattac tgggttaaca cctgatgcac
     1261 ccttgcaact gagcgatctc gttgcgctgg ttcgccaccc tcagggggcg tttaaaaacg
     1321 taccgcaagg ttgtcggcga gtctggttca ttaaccgttt ttctcaatgt gagaatgcga
     1381 ttgcgcaaag cgagctcctt caaccgctgc aacaacacaa cgtagaggca atctggctgg
     1441 gcgatataca agaacatcct gcaatcgcgc gcagatttgt gaattagcgg cccgggtatt
     1501 ccgggttact gaaaggatat ccattgaggt tggtatgaat attttcacag aggctgcaaa
     1561 actcgaagag caaaattgtc cgtttgcgat ggcgcaaatt gttgatagcc gaggctcaac
     1621 cccccgccat tctgcacaaa tgttagtgcg cgccgatggt tctatcgtcg gtacaattgg
     1681 tggcggaatg gttgaacgga aggtgattga agagtcgctt caggcattgc aggaacgtaa
     1741 gccgcgatta ttccatggac gtatggctcg taacggtgcg gatgctgtcg ggtcagattg
     1801 tggaggtgca atgtcagtat ttatcagcgt ccatggtatg cgtccacgtc tggtgttgat
     1861 tggtgcgggg catgtcaacc gggcgatagc ccagagtgcg gcgctattag gatttgatat
     1921 cgccgttgct gatatttatc gcgaaagcct caatcctgaa ctattcccac catcaaccac
     1981 gcttctccat gctgagtcgt ttggtgcggc agtggaagca ctggatattc gccctgataa
     2041 ttttgtcctg attgccacga ataatcagga tcgtgaagcc ctcgacaaac tcattgaaca
     2101 acccattgca tggctggggt tgctggcaag tcgccgcaag gttcagcttt tcctgcgcca
     2161 attgcgtgag aaaggcgtgg ctgaagaaca tattgcccgt ttacatgcgc ccgttggtta
     2221 caacataggt gcggaaacgc cgcaggagat cgccatcagc gtgctggcag aaatattaca
     2281 ggtgaaaaat aacgcgccgg gtgggctgat gatgaagcct tctcatcctt ccggacacca
     2341 gctggtggtg attcgcggtg cgggggatat cgccagtggt gtagcgctac gtctgtatca
     2401 tgcgggtttt aaagtgatca tgttggaagt ggaaaaaccg acagtgattc gttgtaccgt
     2461 ggcgtttgcc caggccgtgt tcgatggcga aatgacggtc gaaggcgtca ccgcccgcct
     2521 ggcaaccagc tctgcggaag cgatgaaact taccgaacgc ggatttatcc ctgtgatggt
     2581 agatcccacc tgttcattgc ttgatgaact gaaaccgctt tgcgtggtgg acgctattct
     2641 ggcgaaacag aatttgggaa cgcgggcaga tatggcacca gtaacaatcg cgcttgggcc
     2701 gggctttact gcagggaagg attgtcatgc ggtaattgaa acaaatcgcg ggcactggct
     2761 cggtcaggtg atttactctg gttgtgcgca ggagaatacc ggtgttcccg gcaatattat
     2821 ggggcatacc acccgacggg tgatccgtgc tcctgctgca ggcattatgc gatccaacgt
     2881 gaaattaggc gatctggtga aagagggcga tgtcattgcc tggattggtg agcatgaaat
     2941 taaagcaccg ttgacgggga tggtgcgtgg cttgttgaac gacggactgg ccgtggtcgg
     3001 tggttttaaa attggtgata tcgatcctcg tggtgaaacg gctgatttca ccagcgtttc
     3061 tgataaagcc cgggcgattg gcggcggcgt acttgaggcg ttaatgatgt tgatgcatca
     3121 gggcgttaaa gcgacaaaag aagtgctgga agtggcttaa atgaaaatgg ccggatggta
     3181 ttacccatcc ggcctttaaa ttactgacaa atcaccgttc cggttttgcc ttcaatccct
     3241 tctttcgctt tgctcaatac ggtaatcagt gcttcacgac ctgcgcgaga gcgagcaaac
     3301 gaggcggcag cttccacttt aggcagcata gaacctttcg caaaatgacc ttcttcgata
     3361 aagcgttcag catcacttaa cgacaggcga tcgagccact gttcattctc ttttccgaag
     3421 ttaatggcca ctttttctac cgcagtcaga atgatcagca tatcggcatc aatcatttct
     3481 gctaaacggg cgctggccca gtctttatcg ataaccgcgc tggcaccacg cagatggtta
     3541 ccttcacgaa taacagggat accaccaccg ccaacggtga tcaccacctg tccggcatct
     3601 accagagctt taaccgtttc tttttcaatg atatcaaccg gttttggcga agcaacgaca
     3661 cggcgatagc cgcgacccgc atcttctttc agagtgtaac cctgttttgt cagtagctct
     3721 gcttcttgct cggtaaagaa cgaaccgatc ggcttggtcg ggttgaggaa tgccggatcg
     3781 ttagcatcga cttcgacctg ggtaaccagc gttgctacag gtttattaat gccacgagaa
     3841 agcagttctt ctcttaaggc gttttgtaga tcgtaaccaa tataaccctg gcttaaggca
     3901 acacagacag acatcggcag cattggcgag tgcgcttcag tttttgctgc agcttcaaag
     3961 gcatggttaa tcatgcccac ctgtggcccg ttgccatgag tgacgatgac ctcatgtccc
     4021 tgggcaatta aatcaacaat tgcctgagaa gtaattttta ccgctttcat ttgcccagcc
     4081 aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa ttttcttact catttttcct
     4141 cactcatcat tcttgattgt gtatagatcc ctggcagtgg ttagagcacg ggagggacaa
     4201 acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc tgtaaaggtg ccgtcgcaga
     4261 aaatcgtttc accacgggat aaggttctga caatcgcgcc ctgacaggta aacccctccc
     4321 atggcgagta gtcagcgttg tcgtggagat ggcgatgctg aatttgttgg ctctgacgtg
     4381 ggtctataat caccacgtcg ccatcggaac cgggcgctaa taatcctttt tgtggccaca
     4441 ggccaaacag cctggcgggc attgcactgg ttaactcaac aaaacgctcc ggtgagatac
     4501 gtcccgtcat cacgccactg gagaacagca gttgcatgcg gttctccaca ccgggtaagc
     4561 catttgggca gcggctgaaa tcgcctttag aaatttgcag gcgttgagcc atcgagaagg
     4621 tgcagtgatc ggttgccacc acgtcaatcg caccatcgct gatgccacac cacagtttgt
     4681 cctgctcgcg tacgttacgc agcggtgggc taagaatgaa cttcatgcca tcttctgtat
     4741 cgtaactgcg ttcgtccaac aggagatatt gtgggcaggt ttcaacccag actggctggt
     4801 gattcgcacg ggcaagacgc agataatcca gacctaagcc gttagacagg tgcacgatat
     4861 agagcggggc gttaccggca atttgtgcca ggttaatcat gcgggcgatg gcttccgctt
     4921 cgcattccag aggtcgactc aaggcatgat aacgcggcgc ggttaaccct gcggcgataa
     4981 actccgcccg cttgctggcg atagctgcgt cattttccgg gtgtacggtg gtcagcgcgc
     5041 cggactcatg caagcggcgt aatgcctgca acacttcatc atcgttgagt ttatattgat
     5101 aggttaaata gagtttaaaa ctgctcagtc cttcctcaac catcatcgga atttcgtcga
     5161 ggatagcgtg attaatatgt tgaatgacac cgtgaaagct gtagtcgatg actgctttgt
     5221 gggcggcata accgcgataa acttccagtt gatggcgtaa ccggcagcca tttgggccaa
     5281 atcccatatg gtcaataatg gttgttgtac cgccacacgc agcagcgcgg gtaccggtaa
     5341 aaaaatcatc acaactgcgc gcgatgccga catcaatatt gaaatgcgta tggacatcca
     5401 cgccgccagg gaaaacgtaa cagccagagg catcaatttc ttcgcaaggg agttgcggcg
     5461 aaatatcggt acccaactgg cgaacaatcc cgctttcaat cagcaaatcc tgcttcgcct
     5521 gtccatctgc attaacaaca atgccgtttt ttatcaatac gcgcatagca aactccaaaa
     5581 accctccggc gaaccggagg ggaataagtg aagagtaatt attcggttgc cagccagctt
     5641 aatgggattg cagcgtacat agcggcacag gttaccaggt gagatttcca ggttttttcg
     5701 tttggcgcgt gcgcttccgg ttctttacca gggccaaaac cgatgaccgg aatgccgtgg
     5761 cgacccatga tggatacgcc attagtcgag aaggtccact tatcaacgac aggcgcttta
     5821 ccaaacagcc cttcataagc attcaccagt gctttaacgg tgaagtgatc ttcttccact
     5881 ttccaggtcg ggaagtagca ttcggttggg taaaccaggc cagtccagga cgggcggtcg
     5941 tagttgtaca tagaaacaac cgcgttagct ttctgtactg caggtagagc gcggatctcg
     6001 tccagcgcgc cttcccaggt ttcgccccag gtcagacgac ggtcgattga aactgcgcag
     6061 ctgtctgcta cagcgcaacg acttggggag gtgaagaaga tttcagaaac agtcagcgta
     6121 cctttgccga ggaattcgtc atagcccaga cgttgagaca gttcctgtaa ttcgccaaga
     6181 attggaccca ttttgaaaat ggcgttatca ccacgttctg gtgccgaacc gtggcagcta
     6241 acaccctgaa catcaatgcg aatttccata cgaccgcgtt gaccacggta tacctggcag
     6301 tcggttggtt cggtactgac cacaaattcc gggcgaatgc cggattgttc aataatgtat
     6361 tgccagcaca gaccgtcgca gtcttcttcc tgcacagtac cggtgaccag cagggtatat
     6421 tcatcttcca gaccgagatc tttaatgatt tttccggcat ataccataga cgccatgccg
     6481 ccttcctggt cggaagtacc acgaccaccg atcagctcgt cggtttccat gccttcatac
     6541 ggatcgaagt cccagttttt gatgttgcca atgccgactg tatcgatatg agcgtccatt
     6601 gccaccagac gcgggccgtg gccgatataa ccgagaacgt tgcccattgg gtcgatttca
     6661 actttatcga agccgacttt ttccatctct tctttaatac gatgtactac gcgtttctcg
     6721 tcgcagcttt cactgggaat agcaaccata tcgcgtagga agcgagtcat gtctgcctgg
     6781 taatcttttg ctttttcaag aatcagtttg aatggaatat tcttagccat tatttttctc
     6841 cagatttttt actccccgtt ttgcagggag cagttccaat agggtgatta aggtgctaca
     6901 gcgtgtttgc cttcccagac aacttcgcgg tagtgcttca cgtcggtgtc gccttcagtg
     6961 ctgataacca gcaccacggc atctttgttc agcgccagtt tttccatcag gctttgacgt
     7021 tgcgggtgat aatgaaccgc tgcgagaacg cccaaaccga cagcgccgga ttcaccggag
     7081 atgatgcgcg ggtcgttgcc atacggatta cccagcacgc gcatacctaa tgcggcaacg
     7141 ctgtcctggc aggagatgaa ttgggtggcg cagttacgta ggatttccca gcccagtggg
     7201 ttaggttcgc cgcaggccag gcccgccatg atggtggcca tatcaccgcc aacgttgacg
     7261 atgtcgcctt tgacgccgga gcgataaata cagtcagctt tgtcaggttc aacaataatg
     7321 ctgtgcagat tttgcgggct atagacgtcg accagataac ccagcacacc accggccatt
     7381 gcaccgacac cggcttgcag cagaacgtgt gtcggggtga cgcccatttc acgcatttgc
     7441 tcgacggctt catccgccag tgttgcgtag ccttgcatga tccaggttgg gattttggtg
     7501 taaccttccc atgccgtgtc ttgtaccact tcccagccgt gctgctgcgc gtgttgcatg
     7561 gtcaggcgaa cggtatcgtc atagttcata tccgtgacga tgcactcggc accgaggttt
     7621 agaatggcgt caacgcgttc ctgagcagaa cctttcggca tgtaaatcac cgcattctgt
     7681 ccgagttgct gtgctgccca tgccacaccg cgcccgtggt tgccgtcggt ggtagtcgcg
     7741 aaagtcattt tttcgccgat ggcatttttc aggtgctcaa atgacagcgt ttcgatatca
     7801 agatgatatt tttcacacaa taattgagcg atggcgtacg caccgccgag cattttgaag
     7861 gcgttcagac cgaatcgttt tgattcgtct ttgacgagaa ttttctttac accaaacagg
     7921 tttgcgagat cgtccagcgc acaaagcggt gttgggtgat aaccagcaat tttctggtgg
     7981 aactggcgtg ccagttttgc ctggctttgc gaaaaaagcg gtgatgtttc gccgttgaaa
     8041 aatttgttat cggcgatatc aatcttcaat gagaaaacgg acatatccta tccttttggc
     8101 ctctggaaat agagggccga ccagctgatc ggcccgaggg gttatttcac gcgttcttgc
     8161 gcttctttca gcagttgctc gagcagtgca cctggtttgg cgtatttacg ggacaggatc
     8221 atcgcggcga tgatataagg cttccagctg gcttctttgt aggtagcgat acggtatttt
     8281 tcgaatacgc cttcagtcac ttcaccttct ttgcaggata cgccgctgat atcagctggc
     8341 aggcagtgca tgtacagggc ttcgccatca cgggtcagtt ccatcatctc ttcagtacaa
     8401 tgccagtctt tgtgttgcgc gttctgtgcc agacactgtt tttccagtgc ttttaagcct
     8461 tcgtgatcgt tcgcacgcag caattcagta cgctcttcca tcactttgta aggtgcccat
     8521 gacttcggat aaacgatgtc tgcgtctttg aaggcttctt ccatgctggt gacctgacgg
     8581 aagctaccac cggaggcttt agcattgttt ttagcaactt caacgacatc cgggatcagg
     8641 tcgtagcctt ccggatgggc cagggtgaca tccataccga agcgggtcat cagacctatg
     8701 atgccttgtg gtacagagag cggtttgcca tagcttgggg agtaggccca ggtcatggcg
     8761 atttttttgc ctttcaggtt ttccagcgaa ccaaagtgtt cacgcaacca tgccaggtca
     8821 gccatagact gagtcgggtg gtcaatatcg cattgcaggt tcactaaagc cggacgctgc
     8881 ggcagcacac cctgcttgta gccgtcatca agtgcagcgc caacttcacg catataggcg
     8941 ttgcctgcgc ccagatacat atcgtcgcga ataccgatag cgtcagcgca gaaggagatc
     9001 atattggcgg tttcacgcac ggtttcgccg tgagcgattt gtgatttacc ttcgtcaaga
     9061 tcttgttgtg caaggccgag caggttaagc gcggaagcat aagagaagcg ggtacgggtg
     9121 gaattgtcgc ggaatacgga aatacctaat ccactattaa agactttggt tgagatgttt
     9181 tcagcacgca gtgcttttaa cgcggcggca acgtccagta cttgtttcag ttcgtctggc
     9241 gtctgttccc acgttaacaa aaaatctttt tcatgcaggt gagaggtcag cgaattgata
     9301 tccttaatca gctcattaac agttttcatc ataaagtccc gatagtggaa tgacaggcaa
     9361 agtggtttat ttcactgtga cgtgccaaag tgattggtgt tttcacagct atacctggga
     9421 ctaacaataa gtgtgccaga attatccacg cggctgcgaa aggctaattt gacggataat
     9481 gtgtggggtt gatcacatat tgatgccagt ttccggttgt ttttaaaata cgaaaacgtt
     9541 tggctttttt tatcacgtta tttatttgct gctaagtatc a
//
LOCUS       DAZFRG010000186         9526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFRG010000186 DAZFRG010000000
VERSION     DAZFRG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.187"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..1019
                     /gene="glaH"
                     /locus_tag="ACHKUC_003653"
     CDS             42..1019
                     /gene="glaH"
                     /locus_tag="ACHKUC_003653"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO6619839.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLPELTFTEQTTNR
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSTGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLCRELMRQRGYFAYATHHYQTHQ"
     gene            1039..2307
                     /gene="lhgO"
                     /locus_tag="ACHKUC_003654"
     CDS             1039..2307
                     /gene="lhgO"
                     /locus_tag="ACHKUC_003654"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO6619840.1"
                     /translation="MYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2330..3778
                     /gene="gabD"
                     /locus_tag="ACHKUC_003655"
     CDS             2330..3778
                     /gene="gabD"
                     /locus_tag="ACHKUC_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6619841.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPTGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3792..5072
                     /gene="gabT"
                     /locus_tag="ACHKUC_003656"
     CDS             3792..5072
                     /gene="gabT"
                     /locus_tag="ACHKUC_003656"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO6619842.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMLRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5309..6709
                     /gene="gabP"
                     /locus_tag="ACHKUC_003657"
     CDS             5309..6709
                     /gene="gabP"
                     /locus_tag="ACHKUC_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO6619843.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6730..7392
                     /gene="csiR"
                     /locus_tag="ACHKUC_003658"
     CDS             6730..7392
                     /gene="csiR"
                     /locus_tag="ACHKUC_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO6619844.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVNLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTATVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7393..7842)
                     /gene="kbp"
                     /locus_tag="ACHKUC_003659"
     CDS             complement(7393..7842)
                     /gene="kbp"
                     /locus_tag="ACHKUC_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO6619845.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILIAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7926..8084)
                     /gene="yqaE"
                     /locus_tag="ACHKUC_003660"
     CDS             complement(7926..8084)
                     /gene="yqaE"
                     /locus_tag="ACHKUC_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6619846.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8267..8566
                     /gene="ygaV"
                     /locus_tag="ACHKUC_003661"
     CDS             8267..8566
                     /gene="ygaV"
                     /locus_tag="ACHKUC_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6619847.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8576..9100
                     /gene="ygaP"
                     /locus_tag="ACHKUC_003662"
     CDS             8576..9100
                     /gene="ygaP"
                     /locus_tag="ACHKUC_003662"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO6619848.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQSGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQSLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9147..>9526)
                     /gene="stpA"
                     /locus_tag="ACHKUC_003663"
     CDS             complement(9147..>9526)
                     /gene="stpA"
                     /locus_tag="ACHKUC_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO6619849.1"
                     /translation="NIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEEEQQQRELAE
                     RQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDVNGETKTWTG
                     QGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2152 a         2477 c         2638 g         2259 t
ORIGIN      
        1 atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca ctgaccgccg
       61 tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc atcccgtcgg
      121 cgcaatcccc gcgtctgccg gaactcacct tcaccgaaca gacgaccaat cggttcctcg
      181 aacaggttgc cgagtggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg
      241 taggcaaaat tctcgacgat ctgtgtgcga atcagctgca accgctgctg ttgaagaccc
      301 tgttaaaccg cgctgaaggt gcgctgttga tcaatgcggt gggtatcgat gatgtcgcgc
      361 aggcggatga gatggtgaag ctggcgacgg cggtggcgca tctgattggt cgctccaatt
      421 ttgacgccat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtt gataactcag
      481 acagctatct tcgtcagccg caccgcgtaa tggagctgca caacgacggc acttacgttg
      541 aagagatcac tgattacgtg ctgatgatga aaatcgacga gcaaaacatg cagggcggta
      601 actcgctgct gctgcatctc gatgactggg aacatctgga ccactttttc cgccatccgc
      661 tggcgcgccg tccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgtct
      721 tccatccggt gttcgatgtc gatcagcagg gccgcccggt gatgcgctat atcgaccagt
      781 ttgtccagcc gaaagacttc gaagaaggcg tgtggttgag cgagctttcg gacgccattg
      841 aaaccagtac aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgattaaca
      901 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg tgccgtgaac
      961 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag
     1021 cgcaaaggaa ttgagcggat gtatgatttt ttgattattg gcggcggcat catcggcatg
     1081 tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa
     1141 gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggagtc
     1201 tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg tgccactaaa
     1261 gtcttttgcg atcaaaacgg cattcgctac gacaactgcg gcaagatgct ggtcgccacc
     1321 tctgaactcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc gaacggtatc
     1381 gagcgcgagt ggttaaacgc cgaggaactg cgcgagcgcg aaccgaatat caccgggctt
     1441 ggcggcattt ttgtgccgtc cagcggcatt gtcagctatc gcgaagtgac ggcggcgatg
     1501 gcaaaaatct tccaggccag aggcggcgag attatttata acgccgaagt cagcgccctc
     1561 agtgagcata aaaacggcgt ggtgatacgt acccgtcagg gcggtgaata tgaagcatcg
     1621 acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa
     1681 ccgggcttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac
     1741 cagattgtta accacctgat ttaccccatt cccgaccccg cgatgccatt tttgggcgtg
     1801 catctcaccc gcatgatcga tggcagcgtg accgtcgggc caaacgcggt gctggctttt
     1861 aaacgcgaag gctatcgcaa gcgcgatttc tcgtttagtg acacgctgga gattttaggc
     1921 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggattggg cgagatgaaa
     1981 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     2041 ttaagcgatc tccagccctg gcccgcaggt gtgcgggcgc aggcggtatc gccggacggc
     2101 aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac ctgcaatgcg
     2161 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     2221 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat
     2281 gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa
     2341 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     2401 tggcgaggtc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtacccaa
     2461 aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgctc tgcccgcctg
     2521 gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca atttgatgat
     2581 ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac cactggctga
     2641 agcgaaaggc gaaatcagct acgccgcctc atttattgag tggtttgccg aagaaggcaa
     2701 acgtatttat ggcgacacca ttcccggtca tcaggccgat aaacgcctga ttgttatcaa
     2761 gcagccgatt ggcgtcaccg cggctatcac gccgtggaac ttcccggcgg cgatgattac
     2821 ccgtaaagca ggtccggcgc tggcggcagg ctgcacgatg gtgctgaagc ccgccagtca
     2881 gacgccgttc tctgcgctgg cgctggcaga actggcgatt cgcgcgggcg ttccgactgg
     2941 cgtgtttaac gtggtcaccg gttcggcggg cgcggtcggt aacgaactga ccagcaaccc
     3001 gctggtgcgc aagctgtcgt ttaccggctc gaccgaaatt ggccgtcagt taatggaaca
     3061 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagttgggc ggcaacgcac cgtttattgt
     3121 ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgtaa
     3181 cgccgggcaa acctgcgtct gcgccaaccg cctgtatgtg cagaacggcg tgtatgaccg
     3241 ctttgccgaa aaattgcagc aggcggtgag caaactgcac atcggcaacg ggctggagaa
     3301 aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat
     3361 tgccgatgcg ctggagaaag gcgcgcgcgt ggtttgcggc ggtaaagcac atgaacgcgg
     3421 cggtaacttc ttccagccga ccattctggt ggacgttccg gctaacgcca aagtgtcgaa
     3481 agaagagacg tttggccccc tcgccccgct gttccgtttt aaagatgaag ccgatgtgat
     3541 tgcgcaagcc aatgacaccg aatttggact tgcagcctat ttctacgccc gtgatttaag
     3601 ccgcgtcttc cgcgtgggcg aagcactgga gtacggcatc atcggcatca acaccggcat
     3661 tatttccaat gaagtggccc cgtttggcgg cattaaagcc tcgggtctgg gtcgtgaagg
     3721 ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact
     3781 ggagaatgcg aatgagcagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc
     3841 gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg
     3901 acgttgaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc
     3961 acctgcatcc gaaagtggtt gcggcggtgg aagcgcagtt gaaaaaactg tcgcacacct
     4021 gcttccaggt gctggcttac gagccgtatc ttgagctgtg cgagattatg aatcagaagg
     4081 tgccgggcga ttttgccaag aaaactctgc tggttacgac cggttccgaa gcggtggaaa
     4141 atgcggtgaa aatcgcccgc gccgccacta aacgtagcgg tactatcgcc tttagcggtg
     4201 cgtatcacgg gcgcacgcat tacacactgg cgctgaccgg caaggtgaat ccatactctg
     4261 cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg
     4321 gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat gacgccgcgc
     4381 cggaagatat cgccgccatc gtgattgagc ccgttcaggg cgaaggcggt ttctacgccg
     4441 caacaccagc ctttatgctg cgtttacgcg cgctgtgtga cgaacacggg atcatgctga
     4501 ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaga
     4561 tgggcgtggc gccggatctc accacctttg cgaaatcgat cgcaggcggc ttcccgctgg
     4621 cgggcgtcac cgggcgcgcg gaagtgatgg acgccgtcgc accgggcgga ctgggtggca
     4681 cctatgccgg taacccgatt gcctgcgtag cggcgctgga agtgttgaag gtgttcgagc
     4741 aggaaaatct gctgcaaaaa gccaacgttc tggggcagaa gctgaaagac ggattgctgg
     4801 cgatcgccga aaaacacccg gagatcggcg acgtacgcgg gctgggggcg atgatcgcca
     4861 tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg
     4921 tggcacgcgc ccgcgataag ggtctgattc ttctctcctg cggcccgtat tacaacgtgc
     4981 tgcgcatcct tgtaccgctc accattgaag acgctcagat ccgtcagggt ctggagatca
     5041 tcagccagtg ttttgctgag gcgaagcagt agcaccgcac catgccggag gcgacgctgc
     5101 gcgtcttgtc cggcctacga gaatccaggt cggataaggc gtttacgccg catccgacaa
     5161 tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg ctctccggca
     5221 aaaagaacca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg gcgctctccc
     5281 aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac atgagttagg
     5341 cggcgggctg aagtcacgcc acgtcaccat gttgtctatt gccggtgtta tcggcgcaag
     5401 tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat tactggccta
     5461 tctgttcgcc ggattactgg tggttatgat tatgcggatg ttggcggaaa tggcggttgc
     5521 cacgcccgat accggttcgt tttccaccta tgccgataaa gccattggcc gctgggcggg
     5581 ctataccatc ggctggttgt actggtggtt ttgggtactg gttatcccac ttgaagccaa
     5641 catcgccgcc atgatcctgc actcgtgggt tccaggcatt cccatctggt tattttccct
     5701 cgtcattacc ctcgccttaa ccggcagtaa tttattaagc gttaaaaact acggcgaatt
     5761 tgagttctgg ctggcgctgt gtaaagtcat cgctatcctg gcctttattt tccttggtgc
     5821 agtcgcaatt agcggttttt acccgtatgc cgacgtgagc ggtatttcaa gattgtggga
     5881 tagcggcggc tttatgccca acggcttcgg tgcggtatta agcgcgatgt tgatcaccat
     5941 gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatccg acacgccgga
     6001 aaaacatatt gtccgcgcca ccaactcggt tatctggcgt atctctattt tctatttgtg
     6061 ttctattttt gtcgttgttg cattaatccc gtggaatatg ccagggctga aagccgttgg
     6121 ttcttatcgc tcggttctgg aattgctcaa tattcctcat gcgaaattaa tcatggactg
     6181 cgtgatatta ctttccgtaa ccagttgcct gaactcggca ctgtataccg cttccaggat
     6241 gctctactcc ttaagccgtc gcggtgatgc tcccgcggta atgggtaaaa tcaaccgcag
     6301 taaaacccca tacgtagcgg tgttactctc caccggcgcg gcattcttaa cggtggtggt
     6361 gaactattac gcgcctgcga aggtgtttaa atttctgatc gacagctccg gcgctatcgc
     6421 cctgctggtt tatttagtca tcgccgtttc acaattgcgg atgcgcaaaa ttctgcgagc
     6481 agaaggaagc gaaattcgcc tgcggatgtg gctttatccg tggctcacct ggctggtaat
     6541 cggttttatt acctttgtgt tggtagtgat gctattccgc ccggcgcaac agttagaagt
     6601 gctctccacc ggcttattag cgattgggat tatctgtacc gtgccaatta tggctcgctg
     6661 gaaaaagctg ataatgtggc aaaaaacacc cattcataat acgcgctgat aatgatcagg
     6721 agtcacacca tgaccattac ttctctggat ggctatcgct ggctgaagaa cgatattatt
     6781 cgcggcaatt ttcagccgga tgaaaaatta cggatgagtt tgctgacatc gcgttatgca
     6841 cttggcgttg ggccgttgcg ggaagcgctt tcgcaactgg tggcagaacg actggtcacg
     6901 gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaagagct gctcgatatt
     6961 ttcgatgccc gcgccaatat ggaagcgatg ttggtgaatc tggcgattgc ccgcggtggc
     7021 gatgaatggg aggcagacgt tcttgcgaag gcgcatctgc tcagtaagct tgaggcctgt
     7081 gacgccagtg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt tcatactgca
     7141 attgtggcgg gctgtggttc ttactatttg ctgcaaatgc gtgaacggtt gtttgatctg
     7201 gcagcgcgtt atcgatttat ctggctgcgg cgaacagtgc tttcggtgga aatgctggaa
     7261 gataaacacg atcagcacca gaccctgact gcgacggtac tggcgcgaga taccgcgcgc
     7321 gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca gcaggcgatg
     7381 gctggcaatt aactactctt caggaatacg caacacttgc cccgggtaaa ttttatccgg
     7441 gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag cgttaccgta
     7501 gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa actggctggc
     7561 agtggctggt gtcgccgttt tcacctgatc atcgacactg gcaataccgg aaatattccc
     7621 caccgcaata aggattttct ccttcgcctc ctgacttaag ccgtcaccag tgaccgtcgc
     7681 tttgccgtcg gcaatttgaa tgttcacttt atcggcatca ggtataccgg ttttgttcag
     7741 atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca
     7801 gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct cattcgttaa
     7861 cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg ggggtgagca
     7921 caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat atagccaagc
     7981 agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag cagcacgccg
     8041 agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac tccctataag
     8101 aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt ttagcggagc
     8161 ctgccagttt cagcgtacgc ttttactctc atttacgcgg ctgaggctgg tatatgttgc
     8221 actcttagtt tagagtttac ttaatttaga aaatacttaa ataactatga ctgaactcgc
     8281 acaattacag gccagtgccg aacaggcagc ggccttattg aaatcaatga gccaccctaa
     8341 acggttgctg attctgtgta tgcttagcgg ttcccccggc accagcgcgg gagagctgac
     8401 gcgcattacc ggactgagtg cctctgcgac atcacagcat ctcgctcgta tgcgggacga
     8461 agggcttatc gacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc
     8521 ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc
     8581 tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat
     8641 cgatattcgt gatgctgatg aatatcttcg tgaacatatt cctgaagcag atctagctcc
     8701 attatccgta ctggaacagt caggtcttcc gcctaaatta cgtcgcgagc aaattatatt
     8761 ccactgccag tcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc
     8821 ccccgcagaa atctttttac tcgaagatgg gattgatggc tggaaaaaag caggattgcc
     8881 agtagcggta aataaatccc aatccttgcc gttaatgcgc caggtgcaga tcgccgcggg
     8941 tggtttaata ttaatcggcg ttgtactggg ttataccgta aatagcgttt tcttcttatt
     9001 aagcggcttt gttggtgctg ggttactgtt tgcaggaatt agtggttttt gtggaatggc
     9061 aaggttgtta gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc
     9121 atagcagcga catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct
     9181 gccagcgcct gagcgattgg cttcggtgta cggccctgac cggtccaggt tttagtttca
     9241 ccgttaacat cgatgaattt atatttcgcc ggacgcggct ggcgtttttt accagcgcgt
     9301 ggtgccgcag cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc
     9361 agctccagcc aggtgctaat tttttcctgg cgctctgcca gttcacgctg ctgttgttct
     9421 tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg
     9481 tcaatggaga attcgcgagc catcgcacga agggtgcgaa tgttat
//
LOCUS       DAZFRG010000187         9487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFRG010000187 DAZFRG010000000
VERSION     DAZFRG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.188"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..1068
                     /gene="ulaG"
                     /locus_tag="ACHKUC_003664"
     CDS             4..1068
                     /gene="ulaG"
                     /locus_tag="ACHKUC_003664"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO6619850.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1176..1931
                     /gene="ulaR"
                     /locus_tag="ACHKUC_003665"
     CDS             1176..1931
                     /gene="ulaR"
                     /locus_tag="ACHKUC_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO6619851.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(1928..2677)
                     /gene="yjfP"
                     /locus_tag="ACHKUC_003666"
     CDS             complement(1928..2677)
                     /gene="yjfP"
                     /locus_tag="ACHKUC_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6619852.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2859..3188
                     /gene="bsmA"
                     /locus_tag="ACHKUC_003667"
     CDS             2859..3188
                     /gene="bsmA"
                     /locus_tag="ACHKUC_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO6619853.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3337..3612
                     /gene="yjfN"
                     /locus_tag="ACHKUC_003668"
     CDS             3337..3612
                     /gene="yjfN"
                     /locus_tag="ACHKUC_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO6619854.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3729..5354)
                     /gene="aidB"
                     /locus_tag="ACHKUC_003669"
     CDS             complement(3729..5354)
                     /gene="aidB"
                     /locus_tag="ACHKUC_003669"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO6619855.1"
                     /translation="MHWQTHIVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     APQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGVRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFLEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5438..6601)
                     /gene="yjfC"
                     /locus_tag="ACHKUC_003670"
     CDS             complement(5438..6601)
                     /gene="yjfC"
                     /locus_tag="ACHKUC_003670"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6619856.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGHNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6604..7242)
                     /gene="yjfM"
                     /locus_tag="ACHKUC_003671"
     CDS             complement(6604..7242)
                     /gene="yjfM"
                     /locus_tag="ACHKUC_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO6619857.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFTSR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7252..7650)
                     /gene="yjfL"
                     /locus_tag="ACHKUC_003672"
     CDS             complement(7252..7650)
                     /gene="yjfL"
                     /locus_tag="ACHKUC_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO6619858.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7668..8327)
                     /gene="yjfK"
                     /locus_tag="ACHKUC_003673"
     CDS             complement(7668..8327)
                     /gene="yjfK"
                     /locus_tag="ACHKUC_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO6619859.1"
                     /translation="MSGFFQRLFGKYNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8378..9076)
                     /gene="yjfJ"
                     /locus_tag="ACHKUC_003674"
     CDS             complement(8378..9076)
                     /gene="yjfJ"
                     /locus_tag="ACHKUC_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO6619860.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITTEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9095..>9487)
                     /gene="yjfI"
                     /locus_tag="ACHKUC_003675"
     CDS             complement(9095..>9487)
                     /gene="yjfI"
                     /locus_tag="ACHKUC_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFO6619861.1"
                     /translation="NPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTS
                     RQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKS
                     SLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2444 a         2500 c         2270 g         2273 t
ORIGIN      
        1 gcgatgagta aagtgaaaag tatcacccgt gaatcctgga tcctgagcac tttcccggag
       61 tggggtagct ggttgaatga agaaattgaa caagaacagg tcgctcctgg cacatttgcg
      121 atgtggtggc ttggctgcac cgggatctgg ttaaaatcgg aaggtggcac caacgtttgc
      181 gttgatttct ggtgcggcac tggcaaacaa agtcacggta acccgttaat gaaacagggt
      241 caccaaatgc agcgcatggc tggcgtgaaa aaactgcagc caaacctgcg caccaccccg
      301 tttgttcttg atccgtttgc gattcgccag gtcgacgcgg tactggcgac tcacgatcac
      361 aacgatcata tcgacgtcaa cgtcgctgct gctgtgatgc agaactgtgc tgatgacgta
      421 ccgttcattg gaccgaaaac ctgtgtggat ttgtggattg gctggggcgt accgaaagag
      481 cgttgcatcg tggtcaaacc gggcgatgtt gtaaaagtga aagacattga aattcatgcg
      541 cttgacgctt tcgaccgtac tgcactgatc accctgcctg ccgatcaaaa agcggctggc
      601 gtactgccgg acggcatgga cgatcgcgcg gtgaactatc tgttcaaaac gcctggcggc
      661 tccctgtatc acagcggcga ctcccactac tctaactatt atgcgaagca cggtaacgaa
      721 catcagatcg acgtggcttt aggttcatac ggcgaaaacc cgcgcggtat caccgacaaa
      781 atgaccagcg ccgatatgct gcgtatgggt gaagccctga atgcgaaagt ggttatcccg
      841 ttccaccacg atatctggtc aaacttccag gccgatccgc aagagatccg cgtgctgtgg
      901 gagatgaaaa aagatcgtct gaagtatggc ttcaagccgt ttatctggca ggttggcggc
      961 aaatttacct ggccgctgga taaagacaac ttcgagtacc actatccgcg cggtttcgat
     1021 gattgcttca ctattgaacc ggatctgccg ttcaagtcat tcctgtaatc tgtcgtttat
     1081 accggatagc gtggagtagt tcgcgctatc cggcgtagta tttcctggct atttcaaata
     1141 tcatctaaaa aaatcaaatt ttatcggaat agctcatgac tgaagcacaa agacatcaaa
     1201 tcctcctgga aatgctcgca caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc
     1261 tgggaatttc gcctgccact gcgcgacgcg atatcaataa acttgacgaa agcggcaaac
     1321 tgaaaaaagt gcgcaatggc gcagaagcta ttacccaaca gcgcccgcgc tggacgccga
     1381 tgaatctgca tcaggcgcag aatcacgatg aaaaagtacg tatcgctaaa gcggcctcgc
     1441 agctggttaa tccgggcgaa agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg
     1501 ggcgggaaat gtgtggcaag ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt
     1561 acctgatcga tcaagaacat gacagcgtga tcattatggg cggacagtac aacaaaagtc
     1621 agtccatcac tttaagcccg cagggcagcg aaaacagtct ctatgccggg cactggatgt
     1681 ttaccagcgg caaaggactg acagcagaag ggttgtataa aaccgatatg ctgacggcaa
     1741 tggcagagca gaagatgctg agcgtggtag ggaaactggt ggtactggtt gatagcagta
     1801 agattggcga acgcgcggga atgcttttta gccgtgccga tcaaattgat atgctgatca
     1861 ccggcaaaaa tgctaacccg gaaatcctgc aacaactgga agcgcaaggg gtcagcattc
     1921 tgcgtgttta aagatgctgg cggaaaaatg tcactgcagc atctaacgcc tcaggcgtaa
     1981 tgcggtgacg cacgcctggc tgccatgaac aggttagcag tttatcccgt cccgtctcgc
     2041 ttaaggcctg ctgcaaacgt agtgattcat cggcaggcac aacgtcatcg tcgaggccat
     2101 gccacagcag tagaggtctg tcaccaagtt gttccaggtg gtttgtcgct tcccactctg
     2161 ccagcggcgc gacaatgtta ttgaattcat tctgttgtgc tgccgtttca ggtatcagcg
     2221 gtggaaacag tgaacgggcg agcgatgtaa aatagcctga tcccatcata ctggcggtac
     2281 atttcaccgt ggggtggcga gcggtaattc ccagtgccgt catcgcgccc atcgacgcgc
     2341 caccgactgc cagacggtca tcacgcagcc agttttcttc ggctattgcc gcacgtaaag
     2401 tagtgaattc ctgcatactt tgtagcaaga tttgccagaa ttgatttaac cgccgcgctt
     2461 cgtcgccact aaaacggcta ccgtgatcgg gcgcatccgg catgatcacc cgcaaaccag
     2521 cctgcgccag cgcaacggca aaatagctat acaccaggct ggatgaagta aagccgtgat
     2581 aaaaaattac gcacggtaac ggggtatctt tttgcccgac aggataagca tgaagaacgg
     2641 gaatatctgc cagctcgcgt gattctattt caatcatctg tctctccttt ttgtctggta
     2701 tgacatgaag aaatccttag cacaagtttc tgctggcggt attagttcat aatgttgaga
     2761 tgtgggttac gctttcgtca cgttttcatt cgaaaatcgc gtaccaggta acaattcggg
     2821 aacattcccc caaagttaaa ttaataaggc actacactat ggttagcagg aaacgtaata
     2881 gcgttattta ccggtttgcc agtttattat tggtgttgat gttaagtgcc tgtagcgcac
     2941 tgcaaggtac gccacagcca gcaccgccag taacggacca tccgcaagag attcgccgcg
     3001 accagacgca agggttacag cgaataggta gcgtaagcac gatggttcgg ggttctccgg
     3061 atgacgcatt agcagaaatc aaagcgaaag ctgtcgctgc aaaagctgat tattacgttg
     3121 tcgtcatggt ggacgaaacc atcgtgacag gacagtggta ttcacaagcc attttgtacc
     3181 gtaaataaat cagacaactt acatagtcag ctttacattg ctttgcgtcc ctatgcgttt
     3241 acctgtgcac aataaattac cggctggaag cctgggcgtt tattaccgcg atggaatgcc
     3301 ctgcaacatg tggggaaacg aaaaatggag ctgacgatga aacaattact tgcctcaccc
     3361 tcgctgcaat tagtgactta tcctgcgagc gccacggcac agtctgcaga attcgccagt
     3421 gctgactgcg tgacgggcct gaatgaaata ggtcagatct cagttagcaa tatttcaggg
     3481 gatccgcagg acgtggaacg tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg
     3541 taccgcatta ttaccatgta cgaagaccag cagcctgata actggcgcgt acaggcaata
     3601 ctgtatgcat gagtgtcatc tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt
     3661 gtccggccta caattttcac atatttcatt gagttaatcg aaaccagcgt cgcatcagtc
     3721 gtatacgctt acacacacac tccccccgtc gcccgcagca ataaatcatt ctggatctgc
     3781 tctgacaacc gtacgccgcc gcgcgtatct aacatcacct gacaccacgc ctgcgccatt
     3841 ggcggagagg catatttcaa catttgcgca ccgcagccca gcaggaatag ctgatgagta
     3901 atttctcgcc ccagttcttc agctggctta cgcagctgct gctgtaaacg acgaaccgcg
     3961 cgatcaaaat aacgatcctg ccctttcact tccaaaaatg cttccgacaa taagtcgtat
     4021 acgcccgctt gcttattgag aacgcgcagc acatccaggc acataatatt gccggaacct
     4081 tcccaaatac tgtttaccgg catctcccgg taaagccgcg gcagctcgct ctcctcgcaa
     4141 taaccaatgc cgcccagcac ctccatcgct tcggccacaa acggcatacc tcgtttgcag
     4201 atcacaaatt ttgccgcagg tgtaaataaa cgcgcccaca gagcttcttt ggcatcggcg
     4261 cgccggtccc acgctcgcgc aagacgaaac agcaacgccg tttgcccttc aagctgaagt
     4321 gccatgcgac ttaagacatg acgcataagg ggctgttgga tcaatggatt accaaaaaca
     4381 tggcgttgat gtgcatgata aatcgccagc gaaaatgcac ggcgcatcat ggcatggcta
     4441 cctagggcgc aatcaaaacg cgtcatcccc cccattttca ggatcagacg aactccttcc
     4501 ccttcctgcc ccaacaacca gccaatggca tcctgaaact ccacttcgca gctggcgtta
     4561 gagcgattac ccagcttatc tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca
     4621 ggcaaaaagc gcggcacaaa aaagcaggac aaaccgcccg tagtctgcgc cagcaccaga
     4681 tgcgcatcgc tttgcggcgc cgagaaaaac catttatgcc ccaccagccg ataagagcca
     4741 tcttccaggc gctctgcgcg ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc
     4801 gtcattccca tgccaatcaa caaaccgcgt ttttgcccac ctggcaataa gtgagaatcg
     4861 tagcgatcgc tcagcagcgg cgtggtccag tcctgaaacg gcgtgggtaa catctgtaac
     4921 aacaatggcg tggcggcaaa ggtcatggtt atcggacata acgacccagc ctcaacctgc
     4981 gcatgtaaca taaaacgcgc cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc
     5041 caggcaagat tgtgcacccg attggtacat agtgcctgca tcagcaggtg ccaggcgggg
     5101 tgaaaacgca catcgtccag acggcgtcct tgcgcatcgt agcgcaataa ttcaggcgga
     5161 ttcacattcg ccagccgccc cagttcaagg gattcagccg ttcctaactg ctgaccaata
     5221 ctggctagaa aatcgctatc ccagccagca ccttcacgcg ttaccgcttc gcagagcgcg
     5281 ccatcagaca ggtataagtt gctgttattt aatggtatag gttgattaaa aacgatgtga
     5341 gtttgccagt gcactgtgtc tccctccatc aatggcagtc accattagta tggtcactgc
     5401 catggattca tgacagaaag agattgctaa aacattctta tccagcaatg taatgcggaa
     5461 cgaaacgtga ggtgtctttg gtgatcagcg tgttatcttc acggatcccc attccgcacg
     5521 cttcatcatc gacaatccag ctaccgatga gtgtgtagct atcgccaaat cgcggcagag
     5581 gttgaaacgc ctggtagatc atcggttcat cggcgtaatc accatcagcg tggtcaacga
     5641 cgttattgtg accgtcgaaa atggtgacat ttccgccttc gcgcgagtag atcggtttac
     5701 gcacatagct ttcgccagcg gcaatctgcg gtttttcacc ctcaaaccag gatgcaagaa
     5761 ggttaggatg accagggaag aagcgccaaa gcagcggcat tagcccttta ttactcaaga
     5821 tacttttcca taacggctcc acccattgct cgcgacgctt gcgcagcagc ggaccattat
     5881 cgtcacgcat catccactcc agcggataca gcttaaatgc acgctggatg acattatcat
     5941 caagatcggt cagtacgccg ccgacgccca aaccgagatc ttcaatgtag ataaaccgcg
     6001 actcctgccc tgcctgctgg gcgcagtcct gcaagtacag cacggtactc ctgtcttcat
     6061 cggtgtcctg acagcagcaa aaataaaacg gttcccgact gtaaagctcg ctaaagcgtg
     6121 aaatcaggcg ttcctgaata gcattgtact gatcggcatc acgcggaata atgccgctgc
     6181 gccgggcatc ctccagccac agccactgga aataagccga ctcgtacaat gaagttggcg
     6241 tatcggcgtt gtactccagc agcttcaccg gcgcattgcc acaccaggca aaatccatcc
     6301 gtccatacag cgaaggatcg cgtgcgcgcc agctttcagc gatcacatcc cagtacaacg
     6361 gcgggatcgc cagttgcgtc aggatctctt catcttttac cgcgcgatcc accacctcaa
     6421 ggcacatctg atgcagttcc gcagtcggtt tttcgatctg ctcttcaatc tggcgcagag
     6481 taaaacggta agcccgactc tcatcccaat agatttcatt gtcgatgata tgaaagtcga
     6541 aaccgttgtt agcggcgatc tggtccagat cccgtcgcac aggaacgttg tgtctcagca
     6601 tgattagcct ccccaatgcc cacgggcgct ggaagaacga ccatagccgc cgcgcgaaac
     6661 ggttgacgct tttttggtgg taaagccgcc tgaagagtaa gactcttttt tgccagaacc
     6721 ggagcgccag gagtaatcac cggaagtgtt gcgccagacg gggcgggaag taaatgagga
     6781 tccgccgctg ttatagacga acggctcatc gcgatcttta cgaataaccc ggcttaacaa
     6841 aaatccagaa acgaccggga tccaactctg ttcaacattg tcgtagtagc aattttcgta
     6901 cttagactga caattctgct gagtcatatt cttcggaaca tcggcataaa atgccgcttt
     6961 ggcgttgttc cagccacgcg cgcaaatgtc ggcattatta ccgtcatcaa tacaatcctg
     7021 cactgtcgcg taaaacgttc cgtcgccgtc gttatcgaca tcgctactat cgctacaacc
     7081 tttcaatacg aaaaaagcgg caccgcccat tagcgccaac gttaagtatt tttgtgcgta
     7141 gcgattgcga cgcggttcaa aaggattatt cggtctacca atgcgactaa tcgctccgtg
     7201 accaatttta ctgttatttc ttgatttgcg ttttctggcc atgatcgtcc attaccatgt
     7261 catacaagct gcgttaaaaa taccgccagc cagcgcggcg gttcccataa acattcctgc
     7321 tgcggtattg tgattaataa ttttttcgct taatgcgggc atataaagtc tgacaccagc
     7381 aaaaacgagt aactgaatca ccagtgcaat cccgccccag gcgaaataat ctggaatact
     7441 caccgcattg attgccgcac tggataaggg gataacgtaa cccaacaatg taccgctgaa
     7501 cgccagtgac gccgcggtat tattgttttt gattaactgc cattcgttgt gcggtgtaat
     7561 tttggagtag ataaacagaa aaataatcac catcgccacg ccaataaaaa aataggcact
     7621 aaaggcaaga agtgaatcca gtatatgcat tgtaaacgtc cttgtaatta accaataata
     7681 tgaagtgatg tcagcgggat atcgacaccc aacgcgcgcg aaaataacca ctctggctca
     7741 ccgagatcgt taaaagattc ctcaccattt agcagcaaat attcgtaagt atcttcggtc
     7801 acttgccgct ggtagcccat ggtaaaatta tggacttccc atttagcatg attttgattt
     7861 tctacttttt ccagcatgta gacgggttcg atatttcccg gttcttcgct gttaaaaaat
     7921 cgctgccagc gtttttcctg ccagtttaag gtcattgccc ccatcgtttt ggcgttgatc
     7981 gcctcgcgcc agtgactctc tttactgata ccgtagctct cttcatagac aaagagcttg
     8041 atatcatcaa tgtcatcaat atcttcgccg ccggtggtat tgatttgcag aaactcatcg
     8101 cctgaagtgt agtagcggaa aatctgacta ccgccgccca gatcgatgtg gctgacagcg
     8161 gctaccgtaa attcctcacc cggcagcgca atcagcaatt catcttccag caaacgaaac
     8221 gctaacgtgt cgagcgtaaa gccactattg agatgaagtc ccagcggacc gcgagcgata
     8281 gcaggcttat tatacttgcc aaacagacgc tggaaaaaac cagacatatt ttatccttaa
     8341 aatagttgcc agccttgcag tgaggctggc aaaaaaatta ctcgccctgt tggcgttgca
     8401 gtcttgctaa tacatcctgg gcgctacttt tattgctacc gccaattccg gcttccgcca
     8461 gcttttcgtc aaggtcgcga ccgtctgcga ctttctccaa ctgtgcggca gcatccaggc
     8521 gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa
     8581 cgctggaaga tgcgccaacg gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt
     8641 cagtggcttt aacgacttcc atttgctgct caaactgagc gatacgctgc gctgtcgctg
     8701 taactgcttt ttccacgcca tcacgggaga cttccagatt cgacaacact tgctcttcag
     8761 tggtaatgag attctcaagg cgcgcgattt cttcagcaac ttcgttaatc aacgacggat
     8821 taacgttctt gcttaacgct tccaacgcac gggcttccag actggctttg cgctcacgta
     8881 aatctttcag cttatcgtga cttaatttca cccgcgccag cagatcaacg cgagattttc
     8941 cggctttatc gagttcagct ttagcgtcac ggatatgctg ttccagcatg cgcacgccct
     9001 gggtttcttc aatggattct tccgcctggg agataaacga tttccccagc gtaaataaac
     9061 tttttaaaat tcccataaat acccgtcctt gtaattagtg tgaatattct tctgtaattt
     9121 cagccagatc caatgcgtta tctaccagcg aggttatctc aagcaggata tcttcaagag
     9181 aagatttaag cgataacgcc ccgaaaacaa tgtaatactc ttcctgttgt acgctggaga
     9241 tccctaccga tgacaacggc atcatcttct gatttcttaa taagaaggta ttaaattcat
     9301 ctggattgct gatactactc accggacaaa taaaggtttc gataatcatt tggcgggaag
     9361 taaaaagaat attgatttgc aaatcgccat aatcattcat tttaataatt aatgcgctct
     9421 cgctatttgt cacatcaata ttttgttcag gtacagtttg cagcgccgtc gccagcgcca
     9481 acggatt
//
LOCUS       DAZFRG010000188         9357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFRG010000188 DAZFRG010000000
VERSION     DAZFRG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.189"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            248..1219
                     /locus_tag="ACHKUC_003676"
     CDS             248..1219
                     /locus_tag="ACHKUC_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO6619862.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFGTLSPEQLEMLHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            1283..1756
                     /locus_tag="ACHKUC_003677"
     CDS             1283..1756
                     /locus_tag="ACHKUC_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6619863.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            1753..3024
                     /locus_tag="ACHKUC_003678"
     CDS             1753..3024
                     /locus_tag="ACHKUC_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6619864.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3067..4731)
                     /gene="tsr"
                     /locus_tag="ACHKUC_003679"
     CDS             complement(3067..4731)
                     /gene="tsr"
                     /locus_tag="ACHKUC_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO6619865.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            5108..7258
                     /gene="btsT"
                     /locus_tag="ACHKUC_003680"
     CDS             5108..7258
                     /gene="btsT"
                     /locus_tag="ACHKUC_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO6619866.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            7376..7579
                     /locus_tag="ACHKUC_003681"
     CDS             7376..7579
                     /locus_tag="ACHKUC_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6619867.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            7590..8546
                     /gene="yjiA"
                     /locus_tag="ACHKUC_003682"
     CDS             7590..8546
                     /gene="yjiA"
                     /locus_tag="ACHKUC_003682"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6619868.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(8632..8823)
                     /locus_tag="ACHKUC_003683"
     CDS             complement(8632..8823)
                     /locus_tag="ACHKUC_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO6619869.1"
                     /translation="MSMVKHKRGTLYTLDVHQEAELKALVNKSDEHIDYSDIPASGSE
                     AWADATRGNFLPDPGIHAQ"
     gene            complement(8864..>9357)
                     /locus_tag="ACHKUC_003684"
     CDS             complement(8864..>9357)
                     /locus_tag="ACHKUC_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619870.1"
                     /translation="YASSLFLSLSNLNITLTPWVEYFWKGMAFMLLFFMLMQLIIYFA
                     FLTLVTKLNVYRLFILAGAFIVSTILVAFASKNVEGISYYVLKSTIDFEWRNQIKCGQ
                     LNISRSNEKYFGFNTDKYTVFYSNREGKWGFDELKCKKGSDRRDAYSIENVSEYNVPG
                     WLK"
BASE COUNT         2255 a         2429 c         2277 g         2396 t
ORIGIN      
        1 ataactaaac tcatcagagg gtgttccttg taacgtcatg cattacataa ctttaagata
       61 tacaatcaaa aaatgcatta aaaaccgatt aaaagcagca aagtattacc cggtgcacga
      121 atttatgata catctgcagc cattctgcta ttcgctgact acttttcagt aatgactgct
      181 ttttcataag gtttcactcc cgacaacaat gttggagttt tatttcctta gcatggagta
      241 agtaaagatg agcaaaataa tagcgatgct aattactgtc agtacccttt ttttaccgtc
      301 aataatccag gcaaaaccta tttcaataaa agttgcctat gaaaataacc ctggagaacc
      361 cctggatgtg gtcatgcgtt actgggcaga cctattaaat aaaaaaagta atggcgaaat
      421 aacacttgcg ctctatccca gctcccaatt gggatcaaaa caggatgtca ccgagcaagc
      481 catgatgggc atgaatgtca taaccttgag tgatgtggct tttcttgccg attatgaacc
      541 cgatttgggg attttgtttg gaccgtatct gactgacgat ccgcaaaaac tgttcaaaat
      601 atatgaaagc gactggttta aacaaaaaaa tgaaagtctg aagaaaaagg gaattcatgt
      661 ggtaatgaat aattatctct atggcactcg acagattatt tcgaaaaaac ccattcgcac
      721 cgttgacgat ctcgcaggtt taaaaattcg cgttcctaat aacgtcatgc aaataaaagc
      781 cattcaggcg atgggggcaa caccaactcc catgccatta ggcgaagttt atcccgcatt
      841 aacccagggc gttattgacg gcgtagaaaa cccaatttca gtgctgcaag ggcaaaaact
      901 gtatgagcag gcaagatatc tgacaatggt taactacctg acaaatacct cagtgtggat
      961 aggtggtgaa gccttctttg gcaccctttc accagaacaa ctggagatgc tccaccagac
     1021 cggctatgaa gcgggtgtct atagccagaa actcactctt gagcgtgatg ccgaaatgtt
     1081 gaaaaccatg caggcagccg gcgtcgaagt gatctatccc gatactggac cattccagaa
     1141 gaaagcacgc gaagtttact ctcaattccc ggaatggaca ccaggtctgt acgaaaccat
     1201 tcaacagcaa ctgcaataag cattaatgct cagcgtcctg cctcatctct cttaggcagg
     1261 acacgcacta acaggaggtt tcatgcgtct ttttggcaaa gtggcagaat ttagtgctgc
     1321 ctttgcgcta ttcgttctgg ttgtggtgac aattggagcg gtatttatgc gttatttcat
     1381 cggccaaccg cttcaatgga cagaagaaat gtcgggaatg ctgatgattt gggtggtgat
     1441 gttaggtgga gtggtggcgg aacgtgacag ggcgcacctg accattccct tcctgatgga
     1501 gatgcttcca ggcaaactca ggcgtgttat tgcagtactg gtcgcccttc tctccatcgc
     1561 actgctgctt tatatggcct ggcttggcta ccgactagcc gaaatggcgc agtttaaagt
     1621 gacacaaatt ctgaaggttt cctggttctg gatcgatctg gcggtccctg tcggcgcact
     1681 ggcaacggct atttataccc tctactggtt aataaacgat acaaaacaat ccgatagcgg
     1741 agacaaaatg caatgagttg gttattgatt attccgctaa tgatcgtctg tttgtttctc
     1801 aacattcggg tctatctggc gatgtttatt ggcatcatcg cttacttttt attcttctcc
     1861 agcgtaccga ttgaaattgc cgtgcaaaga ctaatcgcgc caacgcaaag cccatcattg
     1921 ctggcaattc cgttttttat cttgctcggc acactaatga gctacaccgg cattgccgaa
     1981 cgcatcctgc acattgccaa cgttctggtg gggaaaatgc gcggcggact gggcgtggca
     2041 aacattctcg tcagcaccat gatgggcggc gtcagcgcct ctaaccttgc tgacgcggcg
     2101 atgctgtcgc gcatgatggt gccggagatg gagcgtaaag gctacaatcg cgcgtttgcc
     2161 gccgccatta ccgctggtgg ctcgttagtc accccgatca taccgcctgg aatagccctg
     2221 attatttacg gtctggttgc cgacgtctct atcggaaaaa tgtttatcgc cggactggta
     2281 cctggtttgc tatgcgcagt cgtactgatg tttaccgtct accttgtcgc ccgcaaaact
     2341 aacgctaaac cctcgcgtga aagctggcct acgggcaaag aggtggtgtt ctcgctggga
     2401 caggcctggc ctgcgctgtt cctcattttt gctgtggtgg gtggtattcg cgctaacatt
     2461 ttcaccccaa cagaagcagg tgcagcagcg gtgctaatcg tgctggcgat tggctttttt
     2521 atctatcgtg aaatgcggat ttctcatgtc gtcaaagcat tgggcgaaac ggcacgcgca
     2581 accgcatcgg tgatgctggt aatcatggcc agcgccgcat tgggatggat tttctcaatg
     2641 gaacatgctg gtgtcgcagt ggcgaatttt gtgacttcgc tgaccgaaaa caaatacatg
     2701 ttcctgttga ttatcaacat attgctactg acgctgggga tgtttctgga aggaaacgcc
     2761 attttgctga tcctggtacc actattaaaa ccggtggtcg aacactttgg catcgatccg
     2821 gtgcattttg gcatcatcat gatcttcaac ctgtcgattg gcgcattcac gccaccggta
     2881 ggtaccgtga tgctgctggt atgtaatatc acgcaagttt cggtgggaaa tttctttaaa
     2941 caatccctgc ccctactggc ggctctactg ttaatgctaa tgctggtgac atatatcccg
     3001 gcgatttcac tgttcctggt ttaataataa aaacgccgga tgaaatactc atccggcatc
     3061 attacgttaa aacgtttccc agttctcctc gctatctgcc acggccattt tgcgcggcgt
     3121 agctggcgtc acggttttta ccacagccga tgcttcacgc tgctgttgct gctgttgctg
     3181 aatccggaac actgccactg cttcggtcag gcgactggcc tgctcttcca gcgcggcggc
     3241 ggcggcggca gattcttcca ccagcgcggc gttctgttga gttacccggt ccatctcagc
     3301 aaccgctaag ccaacctgat cgataccacg gctctgctca tcagaagcag aagcaatttc
     3361 gcccataatg tccgtcacgc gggtcacggc actgacaatc tccgccattg tttccccggc
     3421 gctttcgacc agcgtagagc caacatccac tttccccacc gagtcttcaa tcaggctttt
     3481 aatttcacgc gcagcctgag cgctacgctg ggccagatta cgcacttctc ccgcgaccac
     3541 cgcaaaacca cgtccttgct cacccgcacg cgcagcctca accgccgcgt tcaaagccag
     3601 aatattggtc tggaaggcaa taccgtcaat tacgctgata atatcggcga ttttctgcga
     3661 actggtggag atatcgcgca tggtctgcac cacgttatcc accactttgc caccgcgttg
     3721 cgccgtttcg gaagcactta acgccagatg gctggcctgg cgcgcatttt cggcattctg
     3781 tttcaccgtt gcggtcagtt gctccatgct ggctgccgtc tcttccagcg aagcggcctg
     3841 ttgctcggtg cgcgaagaga gatcgttatt gccggtggcg atttcgctgg caccgctata
     3901 gatggcattg gccccgttgc gcacatcacc gacggtacgc atcagctctc cctgcatatg
     3961 gcgcaaactc tctgccagtt gccccatctc attagagcca tccacctcaa tcggtttcac
     4021 cagatcgccg cctgcaatat gacgaatgct gtcaatcagg cgattcattg gcgcgaccag
     4081 cgaggcttta ataccgaacc agacggcgag gatgaccgcc agtacgacga tcatcacgcc
     4141 taccagaatc cacatcgcct ggctgtagga ggcattgtta tcgctgacgg cgatatcata
     4201 gagccgatcg ttttgctcca tgtaagccac atactgcttc tcgaaaccgt cctgatatcc
     4261 ctgggtcggc tgatcaaaga actcgttgat tttgcctgca cctaacagtt gtatcagctc
     4321 cgccagcgca ttgtgataaa tatcgtaatt acgtttgatc tctgccgctg cggcggtgct
     4381 ctgacgcggg tcacgcggca acgcttcgta atccgcccag tttttttccg cctgtttcag
     4441 cgaaatactg gcactctgca tcaactcagc aacggttgaa ccgctaccaa tattgttctg
     4501 atccatcatg tagcggatac ccgcgcggtt gagggtgtta cgcgtctgca acaacgcgac
     4561 ccagctgcca ttcagcgtgg attgctgctg gcgaatggtt tgtaaaacag tgaaattttc
     4621 tttgtcgttc tttaaggcat taaagaacag accgcctgaa gtcagttgta aaaggccaaa
     4681 aacggccaaa accagcagta agctggtcac aattttgata cgttttaaca tggtttctct
     4741 ttcctgtgga ccgatgcaag attttcggcc tggaaaggaa aaactttatg aaaatcccgc
     4801 ggagatgaca tcgagtaatt ttattcaccc cctgaattta aaaagccatt atttacataa
     4861 attattcgca atataaatat gtgatataaa tcacatattt atggttactt aaacgacgcc
     4921 tttgccgctc accgccattc ctgaccgctt acgtccctaa aataaccact cagttattta
     4981 ccttacttta cgcgcgcgta actctggcaa catcactgca ggatagcggt caatttacct
     5041 cctcaaacgc aacgcaaacc tagaacggct tcggccaact attaatcaat acatgccagg
     5101 ttttactatg gatactaaaa agatattcaa gcacataccc tgggtgattc tcggaatcat
     5161 cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gcgccctgtg
     5221 gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat cgctactaca gtctgtacat
     5281 cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga
     5341 cggtctgaac tacgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat
     5401 cgccggtgct ggtccgctgg tgggtccggt tctcgccgcg cagatgggct acctgcctgg
     5461 cacgctgtgg ctgctggcgg gggtcgtgct ggccggtgcg gttcaggact ttatggtgct
     5521 gtttatctcc tctcgccgta acggcgcatc tctcggtgag atgatcaaag aagagatggg
     5581 accagtaccg gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatcctcgc
     5641 cgttctggcg ctgattgtgg ttaaagccct ggccgaaagt ccgtgggggg tcttcaccgt
     5701 ttgctcaacc gtaccgattg cgctgtttat gggtatctac atgcgcttta ttcgtccggg
     5761 gcgtgtgggt gaagtctctg tcattggtat cgtgctgctg gttgcctcta tctacttcgg
     5821 tggcgtgatt gctcacgatc cgtactgggg tccggcactg acctttaaag ataccaccat
     5881 tactttcgcg ctgattggct atgcgtttgt ctccgcactg ctaccagtgt ggctgatcct
     5941 cgcaccgcgc gactatctgg caaccttcct gaaaatcggc gttatcgtcg gcctggcgct
     6001 gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcc atgacccagt acattgacgg
     6061 tactggcccg ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg
     6121 tgcggtatct ggcttccacg cgctgatctc ttccggtacg acgccgaaac tgctggctaa
     6181 tgaaaccgac gcgcgtttca tcggctacgg cgcaatgctg atggagtcct tcgtggcgat
     6241 tatggcgctg gttgctgcgt ccatcatcga accgggtctt tacttcgcga tgaacacccc
     6301 gcctgctggc cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc
     6361 gatcatcatg gcacagctga aagacgttac cgcacacgcg gcagcgaccg tcagctcctg
     6421 gggcttcgtg atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgagccttc
     6481 tgtcctgaac cgtgcaggtg gtgcgccaac gctggcggta ggtatcgctc acgtgttcca
     6541 caaagtgctg ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc
     6601 cctgttcatc ctgaccgcgc tggatgcggg tacccgttct ggccgcttta tgctgcaaga
     6661 cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac tctctggttg ccggtatcat
     6721 cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc
     6781 gctgggcggc gttaagagcc tgtggccgct gttcggtatc tccaaccaga tgctggcagc
     6841 cgtggcgctg gtactgggca ccgttgtact gattaagatg aagcgcaccc aatacatctg
     6901 ggtaactgtt gttccggctg tatggctgct tatctgcacc acctgggcgc tgggcctgaa
     6961 actgttcagc accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga
     7021 gaagattgct aacggtactg acctgacggc gcagcagatt gccaacatga accacatcgt
     7081 tgtgaacaac tacaccaacg caggcctgag tattctgttc ctgattgtgg tgtacagcat
     7141 catcttctac ggtttcaaaa cctggctgaa ggtgcgtaac agcgacaaac gtactgacaa
     7201 agaaacaccg tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc
     7261 ggttgcctta tccggcttac aaaatgtcta tcgtaggccg gataaggcgt ttacgccgca
     7321 tccggcaaat ccccagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt
     7381 tggtaactta ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat
     7441 tccagactac gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat
     7501 gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg
     7561 cggtatgcgc tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg
     7621 gttttttagg cgcaggcaaa accaccctgc tgcgccatat tcttaatgaa caacacggct
     7681 acaagattgc cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg
     7741 gtgatcgcgc cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca
     7801 acgagctgga agacgcccta ctcgacctgc tggataatct cgacaagggc aatattcagt
     7861 tcgaccgtct ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct
     7921 ttttctccca tgagatttta tgccagcgtt acctgctgga tggcgtgatt gcgctggtgg
     7981 atgcggtaca tgccgatgag cagatgaacc agttcaccat cgcccagtca caggttggct
     8041 acgccgaccg tattctgctg actaaaaccg acgtcgcagg cgaagcagaa aaactgcgtg
     8101 aacgtctggc gcgcatcaac gcccgtgcac cggtctacac cgtcacccac ggcgacatcg
     8161 acctgggcct gctgttcaac accaacggtt ttatgctgga agagaacgtc gtcagcacca
     8221 agccacgttt tcactttatc gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac
     8281 tggattaccc ggtagatatc agcgaagttt cccgcgtgat ggaaaacctg ctgctggagt
     8341 cggcggataa actactgcgt tacaaaggga tgctatggat tgacggcgaa cctaaccgcc
     8401 tgctgttcca gggcgtccaa cgcctctaca gcgccgactg ggaccgcccg tggggcgatg
     8461 aaaaaccgca tagtacgatg gtgtttatcg ggattcagct gccagaggaa gagattcggg
     8521 cagcgtttgc ggggttgagg aagtaatcgt gaaaggttga gtgtaaagca acccaatgcc
     8581 ccacttagca gtgaggggga aacagaacga cgtgctcccc tccgctaagt tttattgcgc
     8641 gtgaatacct ggatctggca aaaaattgcc gcgtgtggcg tcagcccatg cttcgctacc
     8701 agacgcagga atgtcgctgt aatcaatatg ttcgtcggat ttattaacca aagctttaag
     8761 ttcagcttct tgatgcacat caagtgtgta gagtgtgccc cgtttatgtt taaccatgct
     8821 catttatgac cagaaccttt actatataat ttataaagat gaattatttc agccatccag
     8881 gcacgttata ttctgagaca ttctcaatgg aatatgcatc tctcctgtca gaacctttct
     8941 tacatttcaa ctcatcaaaa ccccatttac cctctctatt ggaataaaat acagtatatt
     9001 tatcagtatt aaacccaaaa tacttttcat tcgaacgtga aatattcaac tggccacatt
     9061 taatctgatt acgccattca aaatctatgg tgctttttaa aacgtagtac gaaatacctt
     9121 ctacattttt tgaagcaaaa gccactaaaa tagtagatac gataaatgct ccggcaagta
     9181 taaataacct atacacattt aatttagtca ccagagttaa aaaagcaaaa tagataataa
     9241 gttgcattaa catgaagaaa agcaacataa aagccatccc tttccagaaa tactcaaccc
     9301 atggagttag cgtgatgttt agattactca aacttaaaaa caaactggaa gcataac
//
LOCUS       DAZFRG010000189         9341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFRG010000189 DAZFRG010000000
VERSION     DAZFRG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.190"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..718
                     /gene="fetA"
                     /locus_tag="ACHKUC_003685"
     CDS             41..718
                     /gene="fetA"
                     /locus_tag="ACHKUC_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO6619871.1"
                     /translation="MQENSPLLQLQNVGYLASDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVHEQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            705..1484
                     /gene="fetB"
                     /locus_tag="ACHKUC_003686"
     CDS             705..1484
                     /gene="fetB"
                     /locus_tag="ACHKUC_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO6619872.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(1547..2401)
                     /gene="cnoX"
                     /locus_tag="ACHKUC_003687"
     CDS             complement(1547..2401)
                     /gene="cnoX"
                     /locus_tag="ACHKUC_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO6619873.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(2462..3271)
                     /gene="ybbO"
                     /locus_tag="ACHKUC_003688"
     CDS             complement(2462..3271)
                     /gene="ybbO"
                     /locus_tag="ACHKUC_003688"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6619874.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(3261..3884)
                     /gene="tesA"
                     /locus_tag="ACHKUC_003689"
     CDS             complement(3261..3884)
                     /gene="tesA"
                     /locus_tag="ACHKUC_003689"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO6619875.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVINASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            3855..4541
                     /gene="ybbA"
                     /locus_tag="ACHKUC_003690"
     CDS             3855..4541
                     /gene="ybbA"
                     /locus_tag="ACHKUC_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO6619876.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEV"
     gene            4538..6952
                     /gene="ybbP"
                     /locus_tag="ACHKUC_003691"
     CDS             4538..6952
                     /gene="ybbP"
                     /locus_tag="ACHKUC_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO6619877.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNCQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(7193..8287)
                     /gene="mnmH"
                     /locus_tag="ACHKUC_003692"
     CDS             complement(7193..8287)
                     /gene="mnmH"
                     /locus_tag="ACHKUC_003692"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO6619878.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     GYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(8356..9282)
                     /gene="allS"
                     /locus_tag="ACHKUC_003693"
     CDS             complement(8356..9282)
                     /gene="allS"
                     /locus_tag="ACHKUC_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO6619879.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
BASE COUNT         2070 a         2441 c         2584 g         2246 t
ORIGIN      
        1 ataaactgtt tttagtaaaa atcagaaaaa gggaacagtg atgcaggaaa atagtccttt
       61 gcttcagcta caaaacgtag gatatctggc gagtgatgcg aagattctta ataacatcaa
      121 tttttcgctg cgtgctggcg aatttaagtt aattaccggt ccttctggtt gtggcaaaag
      181 tacgctgcta aaaatagttg cttcattgat cagcccaacc agcggaacgt tactgtttga
      241 aggtgaggat gtcagcacac taaagccaga aatctaccgc cagcaagtct cttactgcgc
      301 ccagacgccg acgctgtttg gcgatacggt atacgataat ctgatctttc cctggcagat
      361 ccgtaaccag cagcctgacc cagccatttt tctcgatttt ctcgaacgct tcgctttgcc
      421 ggacaccatt ttgacgaaaa atatcgccga gctatctggg ggtgaaaaac aacgcatctc
      481 attgattcgt aacctgcaat ttatgccgaa ggttttattg ctggatgaaa tcaccagtgc
      541 gctggatgaa agtaataaac ataacgttaa tgagatgatc catcgttatg tgcacgagca
      601 aaatattgcc gtgctgtggg tgacacacga taaagacgaa attaatcatg cggataaagt
      661 gattacactg caaccgcatg ccggagaaat gcaggaagca cgctatgaac tcgcataata
      721 ttactaacga atcattggca ctggcattaa tgctggtggt ggtggcaatc ttaattagcc
      781 ataaagaaaa actggcgctg gagaaagata ttctctggag cgtcgggcgc gcgataattc
      841 agctgattat tgtcggctat gtgctgaagt atattttcag cgtggatgat gccagcctga
      901 cattattgat ggtgttattt atctgcttta atgcggcgtg gaacgcgcaa aaacgcagta
      961 aatatattgc taaagctttt atctcatcgt ttattgccat tacggtcggg gcgggaatta
     1021 ctctggcggt gctgatttta tcgggatcga ttgagtttat cccgatgcag gtgatcccta
     1081 tcgccgggat gatagccggt aacgccatgg tagcggtggg gttgtgttac aacaatttag
     1141 ggcaacgggt tattagcgaa cagcaacaga ttcaggagaa actgagtctt ggcgcgacgc
     1201 cgaaacaggc ttcagcgata ttgattcgcg acagtattcg cgcggcttta attccgacgg
     1261 tcgattcagc aaaaacggtt ggcttagtga gtttaccagg aatgatgtcc gggctgatat
     1321 ttgccgggat tgatccggtg aaggcgatta aatatcagat tatggtgacc tttatgctgc
     1381 tctcaactgc cagcctgtcg accattattg cctgctattt aacctatcgt aaattttata
     1441 attcgcgcca ccaattggtg gtgacgcaat tgaagaagaa atgatgcaat gccggatgcg
     1501 cagcatcgca tccggcgttg tggttcatgt gccgatcaac aaaacatcaa tacaacaacg
     1561 catacagctg gcggcgatac ttcgacgcca gtgcatcacc cgtacccagc gcagcaagga
     1621 tctcctggaa cgttttacgt gtctgaccgt ctgcggcggt gagatcttta cgcagatgcc
     1681 caaacagtaa ctccagcgcc tcttcattgc gcccaacctg gtgcagttgc agtgccagtt
     1741 gcgttgccag tgcggcatct tctggattct ctgccacctg ctgttgcaac tgttgaattt
     1801 ccggcgtatc tgccgcctgc ttcagcagtt cgatttgcgc caccagtccc tggtagcggg
     1861 tgtcctgatc ctgcaatgga atggttttaa gcaccgcttc tgcatcttca gaacggttca
     1921 gcgcaatcag cgtttctgcc aacagcaggc cgatctcccc gttctgattc gacaactgcc
     1981 aggcgtcttt cagcaatggc aaggcgtcgg tgtaattgcc ttcctgcatc agctgcatcg
     2041 cctgctgcgc tttcagctct tcttcgcgcg gcagcacttt atccagcagg gcacggatcg
     2101 cctcttccgg ttgcggcccc tggaagccgt ctactggttg cccgttctgg aacagatata
     2161 cggtcggaat cgcgcgcaga ccaaactggg cggcaatcat ctgctccgcg tcgcagtcca
     2221 gcttcgccag aataaattgc ccgttgtact gcgccgcgag gctttccaga attggggtta
     2281 actgcaaaca gtgctggcta cgttcagacc aaaaatagaa cagcaccgga gtggtcatcg
     2341 actgttcaag aacctgttgc aggttagatt cgttaatgtt gacaatattt tctacggaca
     2401 tggagtcgct ctctgttgtc gatattttct ttgacatggg ggcttaagcg cgcgcttcaa
     2461 ctcacccctg caatattttg tccatcacgc gccccggcag taggcgctta agcaccatta
     2521 ccgcccaggt caccagcgtt accggatagc gcatcttcgg cttctcgcta ataaaagcat
     2581 ggcgcacttt gccgaccacc gcttccggtc ccaacgtaaa gcgggcggcg atgccgggat
     2641 tttcgactgg tttatcactt tgcgtctggt tgacgttgtc ggtgaagcga gtacgaatgg
     2701 gaccaggttc gatcaggctg actttaattc cgctgtggcg cagctccatg cgcagtgcgt
     2761 ctgaccacgc ttccagcgca tatttactgg ccgcgtaagc gccacgaccc ggtgtggaga
     2821 ttaatcccat caccgatgag gtcatcacaa tacgcccttc accgtgcggc aacatcgcgg
     2881 gtaacaggcg catggtgagc tggtgtgcgc cgaaaaagtt ggcggaaaac tgctgttcca
     2941 tctgcgcacg gctgatggtg gaaaggggac catacatgcc gaatccggca ttgttaaaga
     3001 tcccatacag acaattatcg gtcagggcga tcacttcgtc ggctgcgcga tcaacacttt
     3061 ctggcgaatc cagatcgatc aacacgccgg taaatcccat gctgttcatg cgctcaacat
     3121 catccggttt ccggcaacct gccaggacat gaaaaccctg gcgttttaat tcgagcgcgc
     3181 tttccaggcc aataccactg gaacatccgg taattaagac cgatttttgc ataactttac
     3241 ctgtcaggat ctccgttgct ttatgagtca tgatttacta aaggctgcaa ctgcttcgcc
     3301 atccagtcgg caataaacgg ctgggcgtcg cggttgggat gaataccgtc atcctgcatc
     3361 cattgtggct tgaggtagac ctcttccata aaaaagggca gcagcggaac atcaaactct
     3421 ttggcgagtt tggggtaaat ggcgctaaag gcttcattat aacggcgacc atagtttgca
     3481 ggcagacgta tttgcattaa caatggttca gcgttggcgg ctttgacatc ctgcaaaatc
     3541 tggcgcagcg tttgctcggt ttgctgtggc tgaaaaccac gcaaaccgtc attgccgccc
     3601 agttcaacca gcacccagcg cggctgatgc tgtttcagca gagccggaag gcgcgccagc
     3661 ccttgttgcg aggtgtcgcc gctgatgctg gcattgatta ccgacgtttt actctgccac
     3721 ttatcattca acaaggcagg ccaggccgcg ctggcagaca ttcgataccc ggcgctcagg
     3781 ctatcaccca gaatcaataa cgtgtccgct gcggcggcac ggaaggttaa caggaccagg
     3841 aacaggaagg gcaaatgcca gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg
     3901 tcggtcaggg ggagcatgaa ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg
     3961 gtgagaccat tgcactggtg ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc
     4021 tcgcagggct ggatgacggc agcagtggcg aagtgagtct ggtgggacaa ccgctacata
     4081 atatggacga agaagcgcgg gcaaagttgc gcgcgaagca cgtaggcttt gtttttcagt
     4141 catttatgtt aattcctacc cttaacgcgc tggaaaacgt cgagcttccg gcgctgctgc
     4201 gcggtgagag tagcgcagaa agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc
     4261 tgggtaaacg tcttgatcat cttccggcac agctttctgg cggcgaacag caacgagtgg
     4321 cgctggcgcg agcctttaat ggtcgacctg atgtgctgtt tgccgacgag cccaccggca
     4381 accttgaccg ccagaccggc gataaaattg ccgacctgct gttttccctc aaccgtgagc
     4441 atggcaccac gttgattatg gtgacccacg acctgcaact ggcggcacgc tgcgaccgct
     4501 gcttacggct ggtgaacggg cagttgcagg aggaagtatg attgcacgtt ggttctggcg
     4561 cgaatggcgt tcgccgtcgc tattaattgt ctggctggcg ctaagcctgg cggtggcctg
     4621 cgtgctggcg ctgggcaata tcagcgatcg catggagaag ggcttaagcc agcaaagccg
     4681 ggagtttatg gcgggcgatc gggcgttgcg cagttcacgc gaagtgccgc aagcgtggct
     4741 ggaggaagcg caaaagcgcg gcctgaaagt tggcaagcag ctgactttcg ccacaatgac
     4801 ctttgcaggc gacacaccgc agctggcgaa cgtcaaagcg gtggatgata tctacccgat
     4861 gtatggcgat ctgcaaacca atccccctgg cctgaaaccg caggcgggca gcgtattgct
     4921 ggccccacgc ctgatggcac tgcttaacct gaaaacgggc gacaccattg acgtgggcga
     4981 tgccaccttg cggattgccg gagaagtgat tcaggaaccg gattccggtt ttaacccctt
     5041 ccagatggct ccgcgtctga tgatgaatct ggcggatgtc gataaaaccg gagccgtgca
     5101 gccggggagt cgggtcacct ggcgctataa attcggcggc agcgagaacc agctcgacgg
     5161 ctatgagaaa tggttgttac cccagcttaa acctgaacaa cgctggtacg gtctggaaca
     5221 ggacgaaggc gcgctggggc gatcgataga acgttcgcaa cagttcctgc tgctttcggc
     5281 gcttctgacc ttgctgctgg cggtggcggc ggttgcggtg gcgatgaatc attactgtcg
     5341 cagtcgctac gacttggtgg cgatcctcaa gacgctgggg gcagggcgag cgcaactgcg
     5401 taagttaatc gtcggtcagt ggttgatggt gctggcgctt tcagccgtta ccggtggggc
     5461 gataggcctg ttgttcgaaa acgtgttgat ggtgctgctc aagcccgttc tgcctgccgc
     5521 actaccgcct gccagcctct ggccgtggct gtgggcgctc ggcaccatga cagtcatctc
     5581 gctgctggtg gggctgcgac cataccgcct gttgttggca acgcagcctt tacgcgtatt
     5641 acgtaatgat gtggtcgcga acgtctggcc gctgaagttt tatctgccga ttgtcaccgt
     5701 ggtggttgtg ctgctgctcg ccggactgat gggtggcagt atgctgctct gggcggtgct
     5761 ggcgggcgcg gtagtactgg ctttgctgtg cggtgtgctg ggctggatgc tgctgaatgt
     5821 acttcgccgc atgacgctga aatcgctacc tctgcgcctg gcggttagcc gcctgttacg
     5881 tcagccgtgg tcaacgttaa gccagctttc ggcattttcg ctctccttta tgctgctggc
     5941 actgttgctg gtgttgcgtg gcgatctgct cgaccgttgg caacaacaac tgccgccaga
     6001 aagcccgaac tactttttaa tcaacatcgc tacggaacag gttacgccgc tgaaagcgtt
     6061 tctcgcggaa catcagatag tcccggaatc gttttatccg gtggtgcggg cgcggttgac
     6121 ggcgattaac gacaagccga cagaaggcaa tggagatgag gcgctcaacc gcgagctgaa
     6181 tctcacctgg caaaatacgc ggcctgatca taacccgatc gtcgccggta actggccgcc
     6241 aaaagccgat gaagtgtcga tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg
     6301 cgataccgtg acttttatgg gcgataccca ggagttccgc gctaaagtga ccagcctgcg
     6361 caaagtggac tgggaaagtc tgcggcctaa tttctatttt attttcccgg aaggggcatt
     6421 agacgggcaa ccgcagagct ggcttaccag tttccgctgg gagaatggca acggcatgtt
     6481 gacgcaactc aactgccagt tcccgaccat tagcctgtta gacattggcg cgattttaaa
     6541 acaggtcggt caggtgctgg agcaggtaag tcgggcgctg gaagtgatgg tggtgctggt
     6601 caccgcctgc ggtatgttgt tgttgctggc acaggtgcag gtgggaatgc gtcagcgtca
     6661 tcaggagctg gtggtgtggc gcactctcgg tgcgggcaaa aaactgctgc gcacgacatt
     6721 gtggtgtgag ttcgccatgc tcgggtttgt atccggcctg gtggccgcaa ttggtgcgga
     6781 aacggcactg gcggtactgc aatcgaaagt gtttgatttc ccgtgggagc cagactggcg
     6841 attgtggatt gttctgccgt gcagcggtgc gctactgctg tcgctttgcg gcggctggct
     6901 gggtgcgcga ctgcttaagg gtaaggcgct gttcaggcag tttgcggggt gatgacaggt
     6961 gttagattgc ctgatgcgct acgcttatca ggcctacgaa gatcgtgcaa tatattgagt
     7021 tttcgaaatt ttgtaggccg gataaggcgt tcacgccgca tccggcagtt gtgcgccgat
     7081 gccagatgcg acgcaggcgc gtcttatcat gcctacgaaa cagcactcac ctgtaggctg
     7141 gataaggcgt tcacgccgca tccggcatga acagcgtgca cagaatcccc catcactgcg
     7201 ccttaaccca ttccgccact tccgcccact caccgcgaaa gacaactttt tccgcttttt
     7261 tctcaagctg atagcgatac atcgggtcgt aatattcttc aagtaacggc accagccagg
     7321 ccagatgacc gtcggtgctg ccggtggtga gttgcgttgt cagcgccgca tccagccttg
     7381 cagccagttc gttatagcgt tgtagtccca gccgacgttt aatcgccgaa agcccgtgat
     7441 gcaggtattc gcaatacccc tgccagccct gttcgtcgcc ataagcgtgg gtaaaatcat
     7501 gatgcatacg caagaaatac tcttcgttca ggcgttcaag acggatctca aacggatctt
     7561 ctaccaccgc aatcgtcgcc tgagtcattc gttcgcgcag acattccggc aggtgattcg
     7621 aaccgatcat ccggctttca tcttccagca cccacaggcg caaatcctga tgggcgtcgg
     7681 tttttagcat ttcggcagcc agcaggtttt caaaactcgc ctggctaagt tgtggttgta
     7741 acgtgcgacc aaaagcagaa ccgcgatggc gcgccagccc ttccagatca acaccgttcg
     7801 gttgttgttg cactaacagc gttttgccgc agccggtaca accgccaatc agcactatcg
     7861 gtttttgtgc cagttcaata gtcgcctgaa tcgcggtctg gcgcagtgcc ttataaccgc
     7921 cttccaccag cggataatca atccccgcgt catgcagcca gcgttgcaca atatgcgagc
     7981 gctgaccgcc acgggcgcag cagagaatac cgtgcggatt ttgcaggcac gctgcccgcc
     8041 aggcgtctat acgctgctga cgaatttcac ccgccaccag cttatgcccc agtgccagcg
     8101 ccgcgtctga cccttgctgt ttatagcaga tgccaacggc ggcgcgttca tcgttattca
     8161 ttaacggtag gttgatagcg gcgggcattg cgccttgctc aaactcgatg ggggcgcgaa
     8221 catcaataat gggcgtatca gcaatcagca gggcacgata gtcctgttcc gtgtgtctct
     8281 cttgcatagt aaaaagtgaa cctcaaatca gcttgcgcgc tattttacgc gccaacgcgc
     8341 aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt tctaaaaatc cgctgatttc
     8401 cggcctgcgc tgggtaaaca aggtcacaat atcttctacc gctttgccgc tgccaaattt
     8461 gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt gggattaccc ggctgaccag
     8521 ttgttgatta tcgagcattg actggcaaag tgattttggc aaaaaaccaa tgcccacgcc
     8581 cgccagatgg gcggcgattt tcgtttccat atcgggaaca ataatctctt tttgccccgg
     8641 caatcgccag gcgacgcgtt tggttaatgt acgggcgctg tcttcaatat tgaccgccgg
     8701 aaagcgccgc aactgtgctt ctgttagcgg ctcttcaacg ttcgccagcg gatgatccgc
     8761 cgccatgaca aagcgccatt gcaccgatcc taagggatca agactaaagg tatttgccag
     8821 cgcctcggtt cccgtgacgc cgatagccag tgaaaaacct tcgtacaata gcgagtccca
     8881 gacgcccata tagatttgtc gggagatgtg aaattgggta aacgggtaac gttcatttag
     8941 ccacgccagc agcctggcaa cggcctgggg gttatagagc aggttgttga tgacgatatt
     9001 cacctggcgt tccacgccat cattcacctg ttgtaactcg ctgggcatac tttccagcca
     9061 gctcagccag tctctggcct gacaaagtag atgctcgcca gccgccgtca acgtcacgct
     9121 gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct tccagaagtt taatgcgata
     9181 actgatcgtc gccgtggttt tacataatcg ttctgccgct tttgaaaaac ttcctgtttc
     9241 agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac atcttcaggt atcccctttt
     9301 aaatccgcaa gttgcgtgat tttcttatcc tctgatttat c
//
LOCUS       DAZFRG010000190         9297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFRG010000190 DAZFRG010000000
VERSION     DAZFRG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.191"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..504
                     /gene="ndk"
                     /locus_tag="ACHKUC_003694"
     CDS             73..504
                     /gene="ndk"
                     /locus_tag="ACHKUC_003694"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO6619880.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            654..1808
                     /locus_tag="ACHKUC_003695"
     CDS             654..1808
                     /locus_tag="ACHKUC_003695"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO6619881.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            2093..3100
                     /gene="rodZ"
                     /locus_tag="ACHKUC_003696"
     CDS             2093..3100
                     /gene="rodZ"
                     /locus_tag="ACHKUC_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO6619882.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            3127..4245
                     /gene="ispG"
                     /locus_tag="ACHKUC_003697"
                     /gene_synonym="gcpE"
     CDS             3127..4245
                     /gene="ispG"
                     /locus_tag="ACHKUC_003697"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO6619883.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            4356..5630
                     /gene="hisS"
                     /locus_tag="ACHKUC_003698"
     CDS             4356..5630
                     /gene="hisS"
                     /locus_tag="ACHKUC_003698"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO6619884.1"
                     /translation="MAKNIQAIRGMNDYLPGETVIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            5648..6268
                     /gene="yfgM"
                     /locus_tag="ACHKUC_003699"
     CDS             5648..6268
                     /gene="yfgM"
                     /locus_tag="ACHKUC_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO6619885.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            6279..7457
                     /gene="bamB"
                     /locus_tag="ACHKUC_003700"
     CDS             6279..7457
                     /gene="bamB"
                     /locus_tag="ACHKUC_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO6619886.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            7575..9047
                     /gene="der"
                     /locus_tag="ACHKUC_003701"
     CDS             7575..9047
                     /gene="der"
                     /locus_tag="ACHKUC_003701"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO6619887.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            9110..>9297
                     /locus_tag="ACHKUC_003702"
     CDS             9110..>9297
                     /locus_tag="ACHKUC_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6619888.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKNFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLIS"
BASE COUNT         2261 a         2259 c         2599 g         2178 t
ORIGIN      
        1 tgttgctcat tataatccgc gccatctcgt acgctggtac agacaacaac agaacaattt
       61 acagaggtaa aaatggctat tgaacgtact ttttccatca tcaaaccgaa cgcggtagca
      121 aaaaacgtga ttggtagtat ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc
      181 accaaaatgc tgcacctgac cgttgaacag gctcgtggct tttatgctga acacgatgga
      241 aaaccgttct ttgatggtct ggttgaattc atgacttctg gcccgatcgt tgtttccgtg
      301 ctggaaggtg aaaatgctgt tcagcgtcac cgcgatctgc tgggcgcgac caatccggca
      361 aacgcactgg ccggtactct gcgtgctgat tacgctgaca gcctgaccga aaatggtacc
      421 cacggttctg attccgtcga gtctgccgct cgcgaaatcg cttatttctt cggcgaaggc
      481 gaagtgtgcc cgcgcacccg ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg
      541 tgcgccagaa tttgtacaat gcagcgcccc cggacgagca gccgctcacc ggggcgtttc
      601 ttttttcaac cctccagggg ccataacgtg taataacgag gccggaatag catatgtctg
      661 aacaattagt cacacctgaa aacgtcacca cgaaagatgg aaaaatcaac ctgctggatc
      721 tcaaccgtca gcagatgcgg gagtttttta aagatttagg tgaaaaaccc ttccgcgccg
      781 atcaggtgat gaagtggatg tatcactatt gctgcgacaa ctttgatgag atgaccgaca
      841 tcaataaagt gttgcgcggc aaactgaaag aggtggcgga aatccgtgca ccggaagtgg
      901 ttgaagaaca gcgttcatct gacggcacca ttaaatgggc gatcgccgtt ggcgatcagc
      961 gcgttgaaac ggtgtatatc ccggaagacg accgtgccac gctctgcgtc tcttcgcagg
     1021 tggggtgtgc gctggagtgt aaattctgtt ccaccgccca gcagggcttt aaccgcaacc
     1081 tgcgggtatc ggaaattatc ggccaggtgt ggcgtgcggc gaaaattgtc ggcgcagcaa
     1141 aagtcaccgg tcagcgccca atcactaacg tagtgatgat gggtatgggc gagccgctgc
     1201 tcaacctgaa caacgtcgtt ccggcgatgg aaatcatgct tgatgatttc ggttttggcc
     1261 tgtcaaaacg tcgcgtcacg ctttccactt ccggtgtggt tccggcgctg gataaactgg
     1321 gcgatatgat cgacgttgcg ctggctatct ccctgcacgc gccgaacgac gaaattcgtg
     1381 acgaaattgt gccgatcaac aaaaagtaca atatcgaaac gttccttgcg gcggtgcgcc
     1441 gttatctgga gaaatccaac gccaatcagg gccgagttac tattgaatac gtgatgcttg
     1501 atcacgttaa cgacggcact gaacacgcgc accaactggc ggaactgctg aaagatacgc
     1561 cgtgtaagat caacctgatc ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt
     1621 cgaacagccg tatcgaccgt ttctcaaaag tgctgatgag ctacggtttc accactattg
     1681 tgcgtaaaac tcgtggtgat gatatcgatg ctgcttgtgg tcagttggcg ggcgatgtta
     1741 tcgaccgtac gaaacgtacc ctgcgtaaac ggatgcaggg tgaagccatc gacattaaag
     1801 cggtctgata gccattacgc cacggttact ttatgtaccg tggcgtaatc ttttatgaat
     1861 gaagtattaa gttgcaattg tctggtcaat attaacggtg cgtttttgct gactttaagg
     1921 cagtatgtaa cgatgcaaca gtaagttagc cttagtgaat gtgggctttg tcacgagcac
     1981 acagacggtc ttatactgta tgataacggt taacttaacg gatgtttcgc ggtgtgggtg
     2041 ggcatgatgg ttcaccggca tctcaattct catttaaacg tacctgcagc gaatgaatac
     2101 tgaagccacg cacgaccaaa atgaagcact tactaccggc gctcgcctgc gtaatgctcg
     2161 cgaacaacta ggacttagtc aacaggccgt tgccgagcga ctttgcctga aggtttccac
     2221 ggtacgcgac attgaagaag ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg
     2281 atatatccgc tcttatgcgc gtctggtaca tattccagaa gaagaactgc tgccagggct
     2341 ggaaaagcag gctccactac gggctgcaaa agttgcgccg atgcagagtt tttccctcgg
     2401 taaacgccgc aaaaaacgtg acggctggct gatgaccttc acctggctgg tgttgtttgt
     2461 ggttattggc ctgagcggtg cctggtggtg gcaagaccac aaagctcagc aggaagagat
     2521 caccactatg gccgatcaat cttctgcgga actgaataat aatcaaagcc agagtgtacc
     2581 gttagatacg tcgacaacca cagatcaggc tatggcgacc acgccgacat cacctgtgga
     2641 taccaccgca accaacacgc aaacacctgc cgtaactgcg ccagcaccag ctgttgatcc
     2701 gcaacagaat gcggttgttc caccttcgca ggcaaatgtt gataccgctg cgactccggc
     2761 accagcggca acaacaacac cagatggcgc tgcgccgttg ccaaccgatc aggctggcgt
     2821 gaccacgccg gcggttgacc cgaatgcgct ggtgatgaac tttactgccg attgctggct
     2881 ggaagtcacc gatgctaccg gtaaaaaact gtttagcggt atgcagcgta aagacggtaa
     2941 tttgaactta accggccagg caccgtacaa actgaaaatt ggtgcgccag ccgcagtaca
     3001 gatccagtat caagggaaac ctgtcgatct gagtcgtttt atcagaacta accaggttgc
     3061 gcgtctgacc ctcaatgccg aacaatcacc ggcgcagtaa cagacgggta acgcgggaga
     3121 tttttcatgc ataaccaggc tccaattcaa cgtagaaaat caacacgtat ttacgttggg
     3181 aatgtgccga ttggcgatgg tgctcccatc gccgtacagt ccatgaccaa tacgcgtacg
     3241 acagacgtcg aagcaacggt caatcaaatc aaggcgctgg aacgtgttgg cgctgatatc
     3301 gtccgtgtct ccgtaccgac gatggacgcg gcagaagcgt tcaaactcat caaacagcag
     3361 gttaacgtgc cgctggtggc tgacatccac ttcgactatc gcattgcgct gaaagtagcg
     3421 gaatacggcg tcgattgtct gcgtattaac cctggcaata tcggtaatga agagcgtatt
     3481 cgcatggtgg ttgactgtgc gcgcgataaa aacattccga tccgtattgg cgttaatgcc
     3541 ggatcgttgg aaaaagatct gcaagaaaag tatggcgaac cgacgccgca ggcgttgctg
     3601 gaatctgcca tgcgtcatgt tgatcatctc gatcgcctga acttcgatca gttcaaagtc
     3661 agcgtgaaag cgtctgacgt cttcctcgct gttgagtctt atcgtttgct ggcaaaacaa
     3721 atcgatcagc cgctgcatct ggggatcacc gaagccggtg gtgcgcgtag cggggcagta
     3781 aaatccgcta ttggtttagg tctgctgctg tctgaaggca tcggcgacac gctgcgcgta
     3841 tcgctggcgg ccgatccggt cgaagagatc aaagtcggtt tcgatatttt gaaatcgctg
     3901 cgtatccgtt cgcgcgggat caacttcatc gcctgcccga cctgttcgcg tcaggaattt
     3961 gatgttatcg gtacggttaa cgcgctggag caacgcctgg aagatatcat cactccgatg
     4021 gacgtttcga ttatcggctg cgtggtgaat ggccctggtg aggcgctggt ttctacactc
     4081 ggcgtcactg gcggcaacaa gaaaagcggc ctctatgaag atggcgtgcg caaagaccgt
     4141 ctggacaaca acgatatgat cgatcagctg gaagcgcgca ttcgtgcgaa agccagtcag
     4201 ctggacgaag cgcgtcgaat tgacgttcag caggttgaaa aataataacg tgatgggaag
     4261 cgcctcgctt cccgtgtatg attgaacccg catggctccc gaaacattga gggaagcgtt
     4321 gagggttcat ttttatattc agaaagagaa taaacgtggc aaaaaacatt caagccattc
     4381 gcggcatgaa cgattacctg cctggcgaaa cggtcatctg gcagcgcatt gaaggcacac
     4441 tgaaaaacgt gctcggcagc tacggttaca gtgaaatccg cttgccgatt gtagagcaga
     4501 ccccgctatt caaacgtgcg attggtgaag tcaccgacgt ggttgaaaaa gagatgtaca
     4561 cctttgagga tcgcaatggc gacagcctga ctctgcgccc tgaagggacg gcgggctgtg
     4621 tacgcgccgg catcgagcat ggtcttctgt acaatcagga acagcgtctg tggtatatcg
     4681 ggcctatgtt ccgtcacgag cgtccgcaga aagggcgtta tcgtcagttc catcagttgg
     4741 gctgcgaagt tttcggtctg caaggtcctg atatcgacgc cgaactgatt atgctcactg
     4801 cccgctggtg gcgcgcgctg ggtatttccg agcacgtaac tcttgagctg aactctatcg
     4861 gttcgctgga agctcgtgcg aattaccgcg atgcgctggt ggcattcctt gagcagcata
     4921 aagaaaagct ggacgaagac tgcaaacgcc gcatgtacac taacccgctg cgcgtactcg
     4981 attcaaaaaa tccggaagtg caggtgcttc tcaacgacgc tccggcatta ggcgattatc
     5041 tggacgagga atctcgtgag cactttgccg gtctgtgcaa actgcttgag agcgcgggga
     5101 tcgcttacac cgtaaaccag cgtctggtgc gcggtctgga ttactataac cgtaccgttt
     5161 tcgagtgggt gactaacagc ctcggttccc agggcaccgt gtgtgcaggt ggtcgttatg
     5221 acggtctggt ggaacaactg ggtggtcgtg caacaccggc agtcggtttt gcgatgggcc
     5281 tcgaacgtct tgtattgtta gttcaggccg ttaatccgga atttaaagcc gatcctgttg
     5341 tcgatatata cctggtggct tcaggtgctg acacacaatc tgcggctatg gcattagcag
     5401 agcgtctgcg tgatgaatta ccgggcgtga aactgatgac caaccacggc ggcggcaact
     5461 ttaagaaaca gtttgcccgt gctgataaat ggggtgcccg cgttgctgtg gtgctgggtg
     5521 agtctgaagt ggctaacggc acagcagtag tgaaggattt gcgctctggt gagcaaactg
     5581 cagttgcgca ggatagcgta gccgcgcatt tgcgcacgtt actgggttaa ggaaggagaa
     5641 ggacagcgtg gaaatttacg agaacgaaaa cgaccaggtt gaagcggtta aacgcttttt
     5701 tgctgaaaat ggcaaagcac tggctgttgg ggtgattttg ggcgttggcg cactgattgg
     5761 ctggcgctac tggaatagcc atcaggttga ttctgcacgc tccgcttctc ttgcctatca
     5821 aaatgcggtt accgcagtga gcgaaggcaa accggatagc atcccagcgg cggaaaaatt
     5881 tgctgctgaa aataaaaata cttatggtgc gctggcttct ttggaacttg cgcagcaatt
     5941 tgttgacaaa aatgaactag agaaagctgc cgcccagtta caacaggggc tggcagacac
     6001 gagcgatgaa aatctcaaag ccgtgataaa tctgcgtctt gctcgcgttc aggtacagct
     6061 caagcaggct gatgccgcgc taaaaaccct tgatgctatc aaaggtgaag ggtgggctgc
     6121 cattgttgcc gacctgcgtg gtgaagcatt gctgagcaaa ggtgataagc aaggtgcgcg
     6181 tagtgcatgg gaagcaggcg tgaaaagcga tgttaccccg gcactgagcg aaatgatgca
     6241 gatgaaaatt aataatttgt ccatctgaga gggacccgat gcaattgcgt aaattactgc
     6301 tgccaggact gctttccgtt acccttttaa gcggctgttc gctgtttaac agcgaagaag
     6361 atgtggtaaa gatgtcccca ttgccaaccg ttgaaaacca gtttacgccg actactgcgt
     6421 ggagcacttc cgttggtagc ggcattggca acttctattc caatcttcat ccggcactgg
     6481 cggacaacgt tgtctatgca gcggaccgcg ctggtttagt aaaagcgctg aatgcggatg
     6541 acggcaaaga aatctggtct gtcaacctgg ccgagaaaga tggctggttc tctaaagatc
     6601 ctgcattact ttctggcggc gtgaccgtgt ctggtgggca tgtctacatt ggcaccgaaa
     6661 aggcgcaggt ttacgcgctg aataccagcg atggtactgt ggcatggcaa actaaagtcg
     6721 cgggtgaagc actttcgcgc ccggtggtca gcgacggtct ggtgttaatc cacaccagta
     6781 acggtcagtt acaagcgctg aacgaagctg acggcgctgt caaatggaca gttaacctcg
     6841 atatgccttc gctctctttg cgtggcgagt ctgcgccggc aacggctttt ggtgcggccg
     6901 tcgtgggtgg cgataatggt cgcgtcagcg cagtgctgat ggaacagggc cagatgattt
     6961 ggcagcagcg tatttcccag gcgaccggtt ctaccgaaat tgaccgtctg agcgatgttg
     7021 acacgactcc cgtcgttgtt aacggcgttg ttttcgcgct ggcctataat ggtaacctga
     7081 cggcgcttga tctgcgcagc ggtcagatta tgtggaaacg cgaactgggt tcggtgaatg
     7141 atttcatcgt cgacggcaat cgcatctatc tggtcgatca aaatgaccgg gtgatggcgt
     7201 tgaccattga tggcggcgtt acgctgtgga cacaaagcga tctgctgcat cgcctgctga
     7261 cttctccggt gctgtataat ggcaacctgg tggtaggtga cagcgaaggt tatctgcact
     7321 ggattaacgt cgaagatggt cgtttcgttg cccagcaaaa agttgatagt tccggtttcc
     7381 agactgaacc ggttgccgct gacggcaaac tgctgatcca ggcaaaagac ggaaccgtgt
     7441 actctattac acgttaatcg tctctgtcgt tcactttgaa aacggctcct ggacaggggc
     7501 cgttttcctg tttttaacaa cgacacgaat atagcgtgcg ttgtctgatg atttataaaa
     7561 atgaggcttt aaacatggta cctgtggtcg cgcttgtcgg gcgccctaac gtaggaaaat
     7621 ccacgttatt taaccgtcta actcgcaccc gagatgcgct ggttgcggat ttcccgggtc
     7681 tgactcgtga ccgtaagtac ggtcgtgcgg aaattgaagg ccgtgagttt atctgcattg
     7741 ataccggcgg gattgatggc acagaagacg gtgtagaaac ccgcatggcg gaacagtcgc
     7801 tgctggcgat tgaagaagcg gacgtcgtac tgtttatggt ggatgcgcgc gcgggcctga
     7861 tgccggcgga tgaagcgatt gctaaacatc tgcgctcccg tgaaaaaccg accttcctgg
     7921 tggcaaacaa aactgacggt ctggatcccg atcaggcagt ggttgatttc tacgcgcttg
     7981 gtttaggtga aatctacccg atcgccgcgt ctcacggtcg tggcgtatta agtctgctgg
     8041 agcacgtgct gctgccgtgg atggaagatc tcgcaccgca agaagaagtc gacgaagacg
     8101 ccgaatactg ggcgcaattt gaagcggaag agaacggcga agaagaagag gaagacgact
     8161 tcgacccgca aagtctgccg atcaaactgg cgattgtggg tcgcccgaac gtaggtaagt
     8221 ctacactcac taaccgtatt cttggtgaag agcgcgttgt tgtttacgac atgcctggca
     8281 cgacgcgtga cagtatctac attccaatgg aacgcgacgg acgtgagtat gtgctcattg
     8341 acaccgctgg cgtacgtaaa cgcggcaaaa tcaccgatgc ggtagagaaa ttctcggtaa
     8401 tcaaaacgtt gcaggccatt gaagatgcca acgtggtgat gttggtgatt gatgcgcgtg
     8461 aaggtatttc cgatcaggat ctctccttgc tgggctttat tctcaatagt gggcgctcac
     8521 ttgtgattgt agtgaacaag tgggacggcc tgagccagga agtgaaagag caggtgaaag
     8581 agacgctgga cttccgtctg ggctttatcg attttgctcg tgtgcacttt atctctgcct
     8641 tgcacggcag tggtgttggt aacttgtttg aatcagtacg tgaagcgtat gacagctcca
     8701 cccgtcgtgt ggggacctct atgctgacgc gcatcatgac gatggctgtt gaagatcacc
     8761 aaccaccgct ggtacgcggt cgtcgtgtga agctgaaata cgcccacgcc gggggttata
     8821 acccgccgat tgtggtgatt cacggcaatc aggtgaaaga cctgcctgat tcctacaagc
     8881 gttacctgat gaactacttc cgcaaatcgc tggacgtaat gggatcgccg attcgtattc
     8941 agttcaaaga aggggaaaac ccgtatgcga acaagcgtaa caccctgacg ccaacccaga
     9001 tgcgtaaacg taagcgtctg atgaagcata tcaagaaaag taagtaatca cttatctggt
     9061 ttccctttcc acacggagag ggaagccgaa taacacgcga ggagtattta tggcactcaa
     9121 ctgtccaacc tgcaaaaccc ctctcgaacg caatggcgag actgctcact gtgcaacttg
     9181 cgacaaaaac tttaccgtcc aggcactttg ccctgagtgt catcaggcgt tacaagtgtt
     9241 aaaagcttgt ggtgctgtgg attatttctg ccaacatggg cacggtttaa tatctaa
//
LOCUS       DAZFRG010000191         9170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFRG010000191 DAZFRG010000000
VERSION     DAZFRG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.192"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..99)
                     /gene="ykiE"
                     /locus_tag="ACHKUC_003703"
     CDS             complement(31..99)
                     /gene="ykiE"
                     /locus_tag="ACHKUC_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO6619889.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            236..757
                     /gene="fecI"
                     /locus_tag="ACHKUC_003704"
     CDS             236..757
                     /gene="fecI"
                     /locus_tag="ACHKUC_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO6619890.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            754..1707
                     /gene="fecR"
                     /locus_tag="ACHKUC_003705"
     CDS             754..1707
                     /gene="fecR"
                     /locus_tag="ACHKUC_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO6619891.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1794..4118
                     /gene="fecA"
                     /locus_tag="ACHKUC_003706"
     CDS             1794..4118
                     /gene="fecA"
                     /locus_tag="ACHKUC_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO6619892.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4163..5065
                     /gene="fecB"
                     /locus_tag="ACHKUC_003707"
     CDS             4163..5065
                     /gene="fecB"
                     /locus_tag="ACHKUC_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO6619893.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5062..6060
                     /gene="fecC"
                     /locus_tag="ACHKUC_003708"
     CDS             5062..6060
                     /gene="fecC"
                     /locus_tag="ACHKUC_003708"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO6619894.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6057..7013
                     /gene="fecD"
                     /locus_tag="ACHKUC_003709"
     CDS             6057..7013
                     /gene="fecD"
                     /locus_tag="ACHKUC_003709"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO6619895.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNSLLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7014..7781
                     /gene="fecE"
                     /locus_tag="ACHKUC_003710"
     CDS             7014..7781
                     /gene="fecE"
                     /locus_tag="ACHKUC_003710"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO6619896.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8338..8595)
                     /locus_tag="ACHKUC_003711"
     CDS             complement(8338..8595)
                     /locus_tag="ACHKUC_003711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619897.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8678..9170)
                     /locus_tag="ACHKUC_003712"
                     /pseudo
     CDS             complement(8678..9170)
                     /locus_tag="ACHKUC_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1990 a         2626 c         2532 g         2022 t
ORIGIN      
        1 aaatccccat cctttagggc agtgaggatg tcaataatag cagaccacat catgagtcat
       61 tctgaccgac acatgccaga agcagaggct ccatcccatt ttatacctac cttataacac
      121 ttagaaaaac aacatgttaa aaatgtctat tggaaacaat tttatttcca attgtaatga
      181 taaccattct catattaata tgactacgtg ataattaact tttgatgcac tccgcatgtc
      241 tgaccgcgcc actaccacag cttccttaac gttcgagtcg ctttatggca cacatcacgg
      301 ctggttgaaa agctggctga cgcgcaaact ccagtctgct tttgatgcag atgacattgc
      361 ccaggacact tttttgcggg taatggtcag cgaaacgctc tcgacgatcc gcgatcctcg
      421 ctccttcctc tgcactatcg ccaaacgcgt gatggtggac ctgtttcgcc gaaacgcgct
      481 ggaaaaagcg tatctggaga tgctggcgct tatgccggag gggggagcgc cttcacctga
      541 ggaacgcgaa agccaactcg agaccctaca actcctcgac agcatgctgg acgggctaaa
      601 cggcaaaaca cgtgaagcgt ttctgctttc gcaactggat ggtctgacat acagcgagat
      661 tgcgcacaaa ctcggtgttt ccatcagctc cgtgaaaaaa tacgtggcga aagccgtcga
      721 gcactgcctg ctgttccgtc tggagtatgg gttatgaatc ctttgttaac cgattcccgc
      781 cgtcaggcgc tgcgttcagc ttcccactgg tatgccgtgc taagcggcga gcgcgtcagc
      841 ccacaacagg aagcgcgctg gcaacagtgg tatgaacagg atcaggataa ccagtgggcc
      901 tggcagcagg ttgaaaacct gcgcaaccag cttggcggtg tgcctggcga cgttgccagc
      961 cgggcgttgc acgatacccg cctcacccgc cgtcacgtga tgaaaggatt actgctgttg
     1021 ctcggcgctg gcggaggctg gcagctctgg cagtcggaaa ccggcgaagg tctgcgggca
     1081 gattaccgca ccgccaaagg cacagtcagc cgtcagcaac tggaagatgg ctccctgctc
     1141 acgctgaata cccaaagcgc ggcggatgtg cgttttgatg cgcatcagcg caccgtccgg
     1201 ctctggtacg gtgaaatcgc cattactacc gcgaaagatg ccctgcaacg ccccttccgt
     1261 gtcctgaccc gtcagggcca gctcactgct ttagggacag aatttaccgt ccgccagcag
     1321 gataatttca cgcagcttga cgttcagcag cacgctgtgg aagtgcttct cgccagtgcc
     1381 cccgcgcaaa aacgcatcgt gaacgctggt gaaagcctgc agttcagcgc ctctgagttt
     1441 ggcgcagtga aaccgctgga tgacgagagt acaagctgga cgaaggacat cctgagcttc
     1501 agcgataaac cactgggtga ggtgatagcc acgctaaccc gttaccgcaa cggcgtgctg
     1561 cgctgcgatc ccgccgttgc cgggctgcgc ctgagcggga cgttcccgct gaaaaatacc
     1621 gatgcgatcc tgaacgttat cgcgcaaacg cttcccgtta aaattcagtc tattacgcgg
     1681 tactggataa acatttcacc actgtaagga aaataattct tatttcgatt gtccttttta
     1741 cccttctcgt tcgactcata gctgaacaca acaaaaatga tgatggggaa ggtatgacgc
     1801 cgttacgcgt ttttcgtaaa acaacacctt tggttaacgc cattcgcctg agcctgctgc
     1861 cgctggccgg tctctcgttt tccgcttttg ctgcacaggt taatatcgca ccgggatcgc
     1921 tcgataaagc gctcaatcag tatgccgcac acagcggatt taccctctcg gttgacgcca
     1981 gcctgacgcg cggcaagcag agcaacggcc tgcacggcga ttacgacgtc gagagcggcc
     2041 tgcaacaact gctggacggc agcggactgc aggtaaaacc gctgggaaat aatagctgga
     2101 cgctggagcc cgcgcccgca ccaaaagaag atgccctgac cgtggtcggc gactggctgg
     2161 gtgatgcgcg tgaaaacgac gtatttgaac atgctggcgc gcgtgacgtg atccgccgtg
     2221 aggatttcgc caaaaccggc gcaaccacca tgcgtgaggt acttaaccgc atccctggcg
     2281 tcagcgcgcc ggaaaacaac ggcaccggca gccacgacct ggcgatgaac tttggcatcc
     2341 ggggcctgaa cccgcgcctc accagccgct cgaccgtcct gatggacggc atccccgtcc
     2401 ccttcgcccc ttacggtcag ccgcagcttt cactggctcc cgtttcgctc ggcaacatgg
     2461 atgccattga cgtggtacgc ggtggtggtg cggtgcgtta cggaccgcag agcgtgggcg
     2521 gcgtggtgaa ctttgttacc cgtgccattc cgcaggactt tggtatcgag gcgggcgtgg
     2581 aaggtcagct cagcccaacc tcttcacaaa acaacccgaa agagacgcac aacctgatgg
     2641 tgggcggcac agcggacaac ggttttggca ccgcgctgct ctactccggc acgcgcggca
     2701 gtgactggcg cgagcacagc gccacccgca tcgacgacct gatgctgaaa agcaaatatg
     2761 cgccggatga ggtgcacacc ttcaacagcc tgctgcaata ttacgacggt gaagccgaca
     2821 tgcccggtgg cctgtctcgc gcggattacg acgccgatcg ctggcaatcc acccgcccgt
     2881 atgaccgctt ctggggtcgt cgcaagctgg cgagcctggg ctaccagttc cagccagaca
     2941 gccagcataa attcaacatt caggggttct acacccaaac cctgcgcagc ggctacctgg
     3001 agcaaggcaa acgcatcacc ctctcgccgc gtaactactg ggtgcgcggt attgagccac
     3061 gctacagcca gatctttatg atcggccctt ccgcgcacga agtgggcgtg ggctatcgct
     3121 atgtgaatga atcaacgcat gaaatgcgtt actacaccgc caccagcagc gggcagttgc
     3181 cgtccggctc aagcccttac gaccgcgata cgcgttccgg caccgaggcg cacgcctggt
     3241 atctggatga caaaatcgac atcggcaact ggaccatcac gccgggtatg cgtttcgaac
     3301 atatcgagtc ataccagaac aacgccatca caggcacgca cgaagaagtg agctataacg
     3361 caccgcttcc ggcgttgaac gtgctctatc acctgactga cagctggaat ctttatgcaa
     3421 acactgaagg ctcgttcggc accgtacagt acagccagat tggcaaggct gtgcaaagcg
     3481 gcaatgtgga accggaaaaa gcgcgaacct gggaactcgg tacccgctac gacgacggcg
     3541 cgctgacggc ggaaatgggg ctgttcctga ttaactttaa caatcagtac gactccaacc
     3601 agaccaacga caccgtcact gcacgtggca aaacgcgcca taccgggctg gaaacgcagg
     3661 cacgttacga tctgggtacg ctaacgccaa cgcttgataa cgtttccatc tacgccagct
     3721 atgcgtatgt gaacgcggaa atccgcgaga aaggcgacac ctacggcaat ctggtaccat
     3781 tctccccgaa acataaaggc acgctgggcg tggactacaa gccaggaaac tggacgttca
     3841 atctgaacag cgatttccag tccagccagt ttgcggataa cgccaatacg gtgaaagaga
     3901 gcgccgacgg cagtaccggc cgcattcccg gcttcatgct ctggggcgca cgcgtggcgt
     3961 atgactttgg cccgcagatg gcagatctga acctggcatt cggtgtgaaa aacatcttcg
     4021 accaggacta cttcatccgc tcttatgacg acaacaacaa aggcatctat gcaggccagc
     4081 cgcgcacgct gtatatgcag gggtcgttga agttctgatg tgttaacgcc cggcttgccg
     4141 ggcttttagc tggaatgtga ttatgttggc atttatccgt tttgtttttg caggcctgct
     4201 gctggtgatc agccacgcct ttgccgccac ggttcaggac gaacacggca cgtttacact
     4261 cgaaaaaacg ccacaacgga ttgtggtgct ggaactctcg ttcgccgatg cgctggccgc
     4321 cgtggacgtc agcccgatcg gtattgccga cgataacgat gcaaaacgca tcctgcccga
     4381 agtgcgtgcg cacctgaaac cgtggcagtc cgtcggaacg cgcgcgcagc cgagcctgga
     4441 agccattgcc gctctgaaac cagacctgat cattgccgac agcagtcgcc atgcgggggt
     4501 ttacatcgcc ttgcagcaaa tcgcgccggt actgctgctt aagtcccgca acgaaaccta
     4561 cgctgaaaat ttgcaatctg cggctatcat cggcgaaatg gtgggtaaaa agcgagagat
     4621 gcaggcacgc ctggaacaac ataaagagag gatggcgcag tgggccagcc agcttcccaa
     4681 agggacacgc gtggcctttg gcacatcacg cgaacagcaa ttcaacctgc atactcagga
     4741 gacctggacc ggcagcgtgc tggcctctct ggggctgaac gttcccgctg cgatggcggg
     4801 tgcgtccatg ccgtccatcg gcctggagca actgctggcg gtcaatcctg cctggctgct
     4861 ggttgcccac tatcgcgaag agagcattgt taaacgctgg caacaagatc cgctctggca
     4921 gatgttaacc gccgcgcaga agcagcaggt tgcttcggtc gacagtaaca cctgggcgcg
     4981 gatgcgcggt atttttgctg cagagcgtat tgccgctgac acggtaaaaa tcttccacca
     5041 tcagccgctt accgttgtga aatgaccgcg ataaaacacc cggtgctgct gtgggggctt
     5101 cccgttgcag cacttattat tattttctgg ctgagtctgt tttgctactc ggccattcct
     5161 gtttccggag cagatgcaac ccgcgccctg ctgcctggac acacgccaac gctaccagaa
     5221 gcgctggtgc aaaaccttcg tttgccacga agcctggtcg ccgttctgat cggcgcaagc
     5281 ctggcgctcg cgggcacgct gctgcaaacc ctgacccaca acccaatggc ctctccttca
     5341 ctgctcggca ttaacagcgg cgcggcgctg gctatggcgc ttaccagcgc gctgagtccg
     5401 acgccgattg caggctattc tctgtcgttc atcgcggcat gcgggggcgg cgtgagctgg
     5461 ctgctggtca tgaccgcagg aggcggattt cgtcataccc atgacagaaa caaactgatc
     5521 ctcgcgggta tcgcgctgtc ggccttttgt atgggcctta cccgcatcac cctgctgctg
     5581 gccgaagatc atgcttacgg catcttttac tggctggcag gcggagtgtc ccacgcccgc
     5641 tggcaggatg tctggcagct cttgccggtg gtggtcactg cagtccctgt cgtgttgctg
     5701 ctggcgaatc aactgaacct gctcaacctc agcgacagca ccgcccatac gctgggagtg
     5761 aacctgacga ggctacgttt ggtcatcaat atgttagtgc tgcttctggt tggtgcgtgc
     5821 gtcagcgtgg caggtccggt ggcgtttatc ggtctgctgg tgccacatct ggcgcgcttc
     5881 tgggcaggct tcgatcagcg caacgtactg ccagtgagca tgctgctggg ggccacgctg
     5941 atgctgctgg cagatgtact cgcacgcgca ctggccttcc ccggagatct gcccgcaggc
     6001 gcagtgctgg cgctgattgg cagcccttgc tttgtctggc ttgtgaggag gcgaggatga
     6061 aaattgcgct ggttattttc atcacccttg ccctggcggg ctgtgcgctg ttatcactcc
     6121 atatgggagt gatccccgtg ccgtggcgcg cgctgctgac cgactggcag gccggacgcg
     6181 agcattatta tgtattgatg gagtaccgac tgccgcgctt gctgctggca ctgtttgtcg
     6241 gtgcagccct cgccgtggcg ggcgtgctga tacaggggat tgtgcgcaac cctctggcat
     6301 caccggatat tctcggtgtt aaccatgccg ccagcctggc ctctgtgggg gctctacttc
     6361 ttatgccgtc actgcccgtg atggtgctgc cgctgctggc ctttgcgggc ggcatggcgg
     6421 ggttgatatt gctgaagatg ctggcaaaga cccaccagcc gatgaagctg gcgctcaccg
     6481 gcgtggcgct ttctgcatgc tgggccagcc tgacggatta tctgatgctc tcgcgcccgc
     6541 aggatgtgaa caactccctg ctgtggctga ccggcagctt atggggccgt gactggagct
     6601 ttgtgaagat tgccatcccg ctgatgattt tatttctgcc gctgagcctg agtttttgcc
     6661 gcgatctcga cctccttgca ctcggcgatg cgcgcgccac cacgctcggt gtgtcggtgc
     6721 cccatacccg attctgggct ttgttactag ctgtcgccat gacatctacc ggcgtggccg
     6781 cctgcggccc gattagcttt attggtctcg tggtgccgca tatgatgcgt agcatcaccg
     6841 gtggacgtca ccgcagactg ctgcctgttt cggccctgac aggtgcgttg ctgttggtgg
     6901 ttgccgatct gctggcgaga attattcatc ccccactgga gctcccggtt ggcgtgctga
     6961 ccgccattat cggtgcgccg tggtttgtct ggttgcttgt gagaatgcga taaatgactt
     7021 tacgaactga aaatctgacg gtcagttacg ggacagacaa ggtacttaac gacgtttcac
     7081 tctcactgcc aacggggaag atcaccgccc tgatcggtcc taacggttgc gggaaatcga
     7141 cgctgttaaa ctgtttttcg cggcttttaa tgccgcagtc tggcaccgta tttctcggcg
     7201 ataatcccat aaatatgctc tcatcgcgcc agttggcccg caggctttcg ctgctgcctc
     7261 agcaccattt aacgccagag gggatcacag tccaggagct ggtttcgtat ggtcgtaatc
     7321 cctggctgtc actctggggg cgtctctccg ctgaagacaa tgcacgagtt aatgtcgcca
     7381 tgaaccagac ccggatcaat catcttgccg ttcgtcggtt aaccgagctt tccggcggtc
     7441 agcgccagcg cgcatttctg gcaatggtcc tggcccagaa tacgcccgtt gtattacttg
     7501 atgagccaac cacctatctt gatatcaatc accaggtgga cctgatgcgg ttgatgggcg
     7561 aactccggac tcaggggaaa acggtggtcg ctgtgctgca cgaccttaat caggctagcc
     7621 ggtactgcga tcaactggtg gtaatggcaa acggacatgt tatggcgcaa ggcacaccag
     7681 aagaggtgat gaccccagga ttgctgagaa cagtattcag cgtggaagcg gaaatacacc
     7741 ccgagccggt atctggcagg ccgatgtgcc taatgaggta gattgcacag gccgtaagaa
     7801 ccaaaccacg actgaatgaa actggactgg cgccagcaag cctgttcaga ctggggctga
     7861 acttttccgg actctgaaag attaccaata ctcatcgtcc atccgcttgc tttaggctga
     7921 caggttcata atcaacgcaa accagagctg tacaggcttg ggcgcggctt tcaaaccagt
     7981 cgtgatcacg gcaatcaatt ttgaactctg cttaacggac atttctgtat aacccttacg
     8041 gcaacgaaaa acgcgaagtt aaaattttag aaacccaaaa acgtgacatg actaagttta
     8101 gatttcaggg gggagatcaa aaaatttcgc tctgtgccag agcggacatt cacggagctg
     8161 gttcattacc aatgaggttg ggcttttgag gataaatcaa tgatcagacg ccaacgtaaa
     8221 tcaaaagcaa ccctggaacg gaattgctaa tccagtttct gaccatcgat ttttctaaaa
     8281 agtgtgcgtt tgctactact taggtaggtg cagctttctt aatcaccggc agccacgtta
     8341 tacaggccag ttgatggatc gattgttatc aatgatatct tcatgagtcg gtgtctcacc
     8401 cagcttaccg aagctggcat aaagtgaagg cagacgggcc cgtccttctc cctttttcgc
     8461 cagagggaaa gcgcgaagca tggtggcagc ctcccagtca caacaatagg atggtgtgca
     8521 cggctgctga cgccatgatt cagcgataga gccggaaaat acggggtcaa agccggtatc
     8581 attaaccaga gtcatcgtga cctgtactgc ggctggatga tctcctgcta ctgcaactgc
     8641 caggcgaccg cggctccctt caggtagtct gttgtggtca actaaaactg gcctccgcgt
     8701 tagagttttt ccagtatcgg ttttctgatt cgtttggtgg taacccacca ttatattcgt
     8761 gcggtcttag tgcgctgtaa tatccaacga tatagtccgt tattgcgtga gctgcatcgc
     8821 tgaagcttac atagcccgtc gctggcaccc attcgttctt cagactcctg aagaagcgct
     8881 ccattgggct gttatcccag cagtttccac gccgactcat actctgcctg atccggtatc
     8941 gccacagtaa ctgccggaac tgcctgctcg tataatgact gccttgatcg cttggaacat
     9001 caccccgacg ggcttaccac gggtttccca tgccatttcc agtgctttca tggtaagcct
     9061 gctgtccggc gagaacgaca tggcccagcc cactggtttt cttgcgaaca ggtcgagaac
     9121 aacggcgagg tacgcccagc gcttacccgt ccagatatag gtcacatcac
//
LOCUS       DAZFRG010000192         9122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFRG010000192 DAZFRG010000000
VERSION     DAZFRG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.193"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..722
                     /gene="ecpR"
                     /locus_tag="ACHKUC_003713"
                     /gene_synonym="matA"
     CDS             180..722
                     /gene="ecpR"
                     /locus_tag="ACHKUC_003713"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO6619898.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            797..1384
                     /gene="ecpA"
                     /locus_tag="ACHKUC_003714"
                     /gene_synonym="matB"
     CDS             797..1384
                     /gene="ecpA"
                     /locus_tag="ACHKUC_003714"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO6619899.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1441..2109
                     /gene="ecpB"
                     /locus_tag="ACHKUC_003715"
                     /gene_synonym="yagY"
     CDS             1441..2109
                     /gene="ecpB"
                     /locus_tag="ACHKUC_003715"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO6619900.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2135..4660
                     /gene="ecpC"
                     /locus_tag="ACHKUC_003716"
                     /gene_synonym="yagX"
     CDS             2135..4660
                     /gene="ecpC"
                     /locus_tag="ACHKUC_003716"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO6619901.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGEAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSSTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4650..6293
                     /gene="ecpD"
                     /locus_tag="ACHKUC_003717"
     CDS             4650..6293
                     /gene="ecpD"
                     /locus_tag="ACHKUC_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO6619902.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6262..6972
                     /gene="ecpE"
                     /locus_tag="ACHKUC_003718"
     CDS             6262..6972
                     /gene="ecpE"
                     /locus_tag="ACHKUC_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO6619903.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7285..7614
                     /gene="ykgJ"
                     /locus_tag="ACHKUC_003719"
     CDS             7285..7614
                     /gene="ykgJ"
                     /locus_tag="ACHKUC_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6619904.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7626..7792)
                     /locus_tag="ACHKUC_003720"
                     /pseudo
     CDS             complement(7626..7792)
                     /locus_tag="ACHKUC_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7863..8477)
                     /gene="yagU"
                     /locus_tag="ACHKUC_003721"
     CDS             complement(7863..8477)
                     /gene="yagU"
                     /locus_tag="ACHKUC_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO6619905.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8692..8877)
                     /locus_tag="ACHKUC_003722"
     CDS             complement(8692..8877)
                     /locus_tag="ACHKUC_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619906.1"
                     /translation="MASGLICHGGFEPHAWGVANLMDVAVLVVAYLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8926..9084
                     /locus_tag="ACHKUC_003723"
                     /pseudo
     CDS             8926..9084
                     /locus_tag="ACHKUC_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2461 a         2306 c         2246 g         2109 t
ORIGIN      
        1 atttaaacct aatcgaaata attaaaactt aatctcgttt aacctatatt gatatgtgct
       61 acgtatctta tttacttccg atttactaaa gaaactgaat gtacctgtaa aaattacagg
      121 tttggaaagt agtgacatgg caaaatgatt acagcaggga ctatgaggtt aaaaaccata
      181 tggaatgtca aaaccgttct gataaataca tctggtctcc ccatgacgcc tacttctata
      241 aaggactatc tgaactgatt gtggatatcg acagattaat ttatctatcg ctggagaaaa
      301 tcagaaaaga tttcgtgttt atcaatctca atacggattc tttaactgag tttataaacc
      361 gtgataatga gtggttatcc gcggtaaagg ggaaacaggt cgtattgatt gcggccagaa
      421 agtcagaagc cttagcaaat tattggtatt acaacagcaa tattaggggc gtggtatacg
      481 ctggactgag tcgtgatatt agaaaagaac tggcctatgt gattaatggc aggttcctga
      541 gaaaagatat taagaaagat aaaatcactg accgggaaat ggaaattatc cgcatgacgg
      601 ctcagggaat gctgcctaaa tcgattgcca gaattgaaaa ttgtagtgtg aagacagtgt
      661 atacccatcg gcggaatgca gaggccaagc tgtactcaaa attatataag ttggttcagt
      721 aaactccagg caagttagtt ttaaaaaatg actcaccggg acttcatgtc ctcaattcaa
      781 ctcgggaaga aaagcaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac
      841 cggtacaggt gtagcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcagc
      901 aacagccaaa aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt
      961 ccagtatgcc gaaggcatta aaggttttaa ctcacaaaaa ggtctatttg acgtggctat
     1021 cgagggtgac tcaacggcta ccgcctttaa actgacctca cgtcttatca ccaacacctt
     1081 aacccagttg gatacctcag gttccacact gaatgtgggc gtggattata acggcgcggc
     1141 agtcgaaaaa actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa
     1201 ccttagccca ctggctaacg gttacaatgc cagcaatcgt accaccgcac aggatggttt
     1261 cactttctcc atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg attacagcac
     1321 tctaccggaa ggcatctgga gcggcgacgt tagcgtacag ttcgacgcta cctggaccag
     1381 ttaatctctc tgatgtacca gcaggggtac ccccctgctt tattcctgga cggaattgtt
     1441 atgaaaaagc accttctgcc tctcgctctg ctgttttccg gaatatcacc ggcccaggcg
     1501 ctggatgtcg gcgatatatc atcgtttatg aacagtgaca gcagcacgct gagcaaaacg
     1561 atccaaaaca gtaccgacag tggccgcctc atcaatatcc gtctcgaacg gctctcttca
     1621 ccgcttgacg acgggcaggt tatcgcaatg gacaagccgg atgagttgct actcactccc
     1681 gccagcttgc tgctacccgc ccaagccagc gaagtgatcc gcttcttcta taagggacca
     1741 gcagatgaaa aagagcgcta ctaccgcatt gtctggtttg atcaggccct cagtgatgcg
     1801 cagcgcgata atgccaaccg tagcgctgtg gccactgctt ccgcccgcat cggcaccatt
     1861 ctggtcgtcg caccccgcca ggcgaactac cactttcagt acgccaacgg ctccctgaca
     1921 aatacaggaa atgcgacgct gcggatcctc gcctacggcc cctgcctgaa agccgccaat
     1981 ggtaaggagt gtaaagagaa ttactacctg atgccgggca agtcgcgtcg ttttacccgc
     2041 gtggacacgg cggataacaa aggacgggtt gcactttggc agggtgataa gttcattccc
     2101 gtgaaataga tagctgtgca gatggataac gacaatgcct ttacgacggt tctccccagg
     2161 actgaaagcc cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc
     2221 tgctgacata agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc
     2281 gcttcaggac ggcatgagcg tcccgctcta tattcatctc gccggtagcc agggtcgcca
     2341 ggacgatcag cgaatcggca gcgcttttat ctggttggat gatggacagt tacgcatccg
     2401 gaaaatacag ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca
     2461 actgatgact ctggccaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa
     2521 cgcgcagttg gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc
     2581 gctgggcacc gtactacgct cacgtagcga agacatcggg cagtccagtg ttaataccct
     2641 cagcagtaat ctgagctata acttcggcgt ctataacaac cagttgcgta acggcgggag
     2701 caacacctcc agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcacgtggt
     2761 gctcgacggc tcactgtacg ggattggtag cggtcaacag gacagtgaat tatataaagc
     2821 gatgtatgaa cgcgattttg ctggtcaccg atttgccggt ggaatgctcg acacctggaa
     2881 cttgcagtcc ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg
     2941 gggaaaccag gccagctcca ccatcttcga cagcagccag tcggccacgc cagtgatcgc
     3001 ctttttaccg gcggcgggcg aagtacatct cacccgtgat gggcggctac taagcgttca
     3061 gaacttcacc atgggcaatc atgaagtgga tacccggggt ctaccgtacg gtatttacga
     3121 tgtggaagtt gaggtaatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa
     3181 taagctgttt agccggggac gtggcgtcgg tgcaccgctg gcgtggcaga tatggggcgg
     3241 tagctttcat atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ccaaagagag
     3301 ttggctggca ggtgcctcga cctccggctc actgagtacg tttagctggg cggcaacggg
     3361 atatggatac gataatcagg cggtgggtga aacccgtctg acgctgccgc ttggggaggc
     3421 gatcaacgtt aacctgcaaa acatgctggc cagtgacagc tcatggagca acatcgccag
     3481 catcagcgcc actctacctg gaggcttcag ttcgctgtgg gttaaccagg aaaaaacccg
     3541 cattggcaat caattgcgac gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa
     3601 cctgaactca ctgtggtcga aactgggtac gttcagcatc agctacaatg atgaccgccg
     3661 ctacaacagc cattattaca cggcagatta ctatcaaagt gtctacagca gtacctttgg
     3721 ttcgcttggc ctgcgggccg gtattcagcg ctataacaac ggcgacagca gcgccaatac
     3781 agggaaatat atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat
     3841 gacccatcaa aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg
     3901 aaccattcgc actgttggtg ccaatctgtc acgagccatc tccggcgata ccggtgatga
     3961 caaaaccctc agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct
     4021 gaacgtcaat agcgcggcgg acggctacat caataccaat ttgaccgcca acggcagcgt
     4081 cggctggcag ggtaaaaaca tcgctgccag cgggcggacc gatggcaacg ctggggtgat
     4141 attcgacact ggcctggaga acgatggtca gatcagcgcc aaaatcaacg ggcggatttt
     4201 cccgcttaac ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga
     4261 gttgcagaac agcaaaaact cactcgacag ttacgatatc gtcagcggtc gcaaaagtca
     4321 tctgactctc tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac
     4381 cgtctccggt cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa
     4441 ccatatcggc cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa
     4501 ataccccact atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact
     4561 caaccaggcg cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg
     4621 ggcggcggtg acgcagacag gagaagagaa tgagagttaa cctactgata gcgatgataa
     4681 tctttgcgct aatctggcct gcaaccgcgc tcagagcggc agtgagcaaa acaacctggg
     4741 cggatgctcc ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg
     4801 tcactcctgg cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa
     4861 aatacaatgg ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca
     4921 acaccggcct ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat
     4981 ctcctttaac tggcttgcgc tgcatcaact ggtacgcagg gtgtaatatg accaccagtc
     5041 ttatcctgcc gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg
     5101 gcgcgaaatg gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc
     5161 ccgtcggcag cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg
     5221 ccagcagcgg cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca
     5281 cccatacgaa agcagcaaat ctgcggttga taaataccca ctcgctggcg gaagtattta
     5341 tcaacagcga cggagtaccg actctgggcg aagggaacgc cgactgccgg acgcaaacca
     5401 tcggcagccg ttcaggatta agttgtaaga tggttaacta taccctgcaa acaaacggac
     5461 tcagcaacac ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg
     5521 tcggggcgta cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata
     5581 ctgcctatta ctacaccttc aacgagatga agagcgcaga ctcgatctat gttttcttct
     5641 cgagcaactt ctttaaacag atggtgaacc tcgggatcag cgatatcaac accaaagatc
     5701 tattcaactt tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta
     5761 cctccaacac gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat
     5821 atactcagac accgtcgcgg gagggatatg tcggcagcgg cgagtcggca ctcgatttcg
     5881 gctatatcgt aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg
     5941 gacccgcgca ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg
     6001 ctaaagtacc gttcccggcg acgctttcct ttattacccg caacggagct acaaaaacct
     6061 acgatgccgg gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac
     6121 cgtggactga tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga
     6181 ttccaatgga taacgccatt tctctgcgta cggtagatga taacggctgg tttggcgaag
     6241 tcagcgcttc aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg
     6301 ctgacagtgg tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg
     6361 actttttcac tgccgtccga gactgacttt gtcagcaaac gtgtcgtgaa taacaacaaa
     6421 agcgcgcgga tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg
     6481 cgcacccgac cggtggatgg tgaactactt ttcgcccccc gccagctggc gttgcaggct
     6541 ggtgagagcg agtattttaa attttactat catggtccac gggataaccg cgagcgctac
     6601 taccgggtct catttcgcga ggtccccact cgtaacctga caaaacgcag cccaaccggc
     6661 ggtgaggtca gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt
     6721 caggttcagt ttaaatggtc cttcgatcag gtgaccggaa cggtaagtaa caccggcaac
     6781 acgtggttta agctactgat taaaccagga tgtgattcga ccgaagagga aggcgatgcc
     6841 tggtatctac gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat
     6901 tatctggtct ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct
     6961 tcggcggact aagacttatc catcagacga gggaagaatt ccacctcaga gactcaaaaa
     7021 tcgtttaaat gatgtggaag cgatcgtcaa tgtcacctgt aacgatgagc tttctcatat
     7081 gcacaccaca gtgttcagga gacacggaaa ggtaccaggc ggcctgctaa cgagtggttt
     7141 cttcactaat taggacgtaa aatcggtaac ggctggaaat cattcaatac tcacactatc
     7201 gaaagtacac cagccaaccg cgatacgtcc ctgcatacga cgtaccgcag ttctctttac
     7261 gatttatagc tgtactggtg aattatgagc aatctgaatc catgcatgac gtgtggtgcc
     7321 tgttgtgcat ttttccgcgt ctctttttac tgggccgaag ccgacgatgc tggcggtacc
     7381 attcccgcca ggcttactga acaaatatcc ccttttcacc gatgcatgag cggtaccaat
     7441 cagaaaaacc cccgatgtat tgcccttgca ggaacaccgg gcaaaaatgc ctgttgcacg
     7501 atatataaaa atcgatcgtc cacatgcaga gaattcgcca tgtctggtga gaacggagaa
     7561 gtcaatgagg cttgcaatcg tgcaagagct aaatacgggc taacaccgtt ataaatatac
     7621 aataattaat tgcattgccc cgccagctgt aatagcgggg cttcgttttt cagggacaac
     7681 aaaacctgaa acttcttcca ttttccagca acaatgcctc ttcttctgta gcgttctccg
     7741 gatctacatc atcctgaagt agcgcgatat gatcactgac tgtccgcatc agaatatgaa
     7801 aagcaacgtt tttaacctat aaaaatggcg ctatatttgc gccattttta tcattcaaag
     7861 cattatctat ttgagcctaa agggatctca ggatctggct catgggtaat tctgtttcgt
     7921 aaatctctgc gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagag
     7981 acattctcat accacggaag atcaaagaga ggtggcgtaa gtcccatgag agggaatgaa
     8041 atcatatgaa caaaaagttg cgctaaagca cctgccagta agccctgcca aagtttaatt
     8101 ttcggaaata cttcggcgac cacacagtaa ccaacggcaa aaactatcga aaagattatg
     8161 tgcgttacac caacccagtt aaagacatgc ccggcgaagg tataaacagc cgaatttgga
     8221 tctgtcagtc ccaaccaatc ccgaagaaaa atgtacggag gattgagaaa gttacgggag
     8281 caatcaattt ggcctgcagc cctgattaat gattccggac cacacgctgc attaaacata
     8341 tcaaccggac tacgtggtgg caatggaact tctgcccccc acttaacaaa tgcggaaaca
     8401 acgccagcaa tcagcccaat gaatgcagca agaccataac gtctgcggtt tggtggagtt
     8461 tgttcaaata tattcatatc taccctgctt gtaccattat gttatacacc tctttaggag
     8521 tattcataaa acaaggcaaa tgtaaagaac tgtattgttt tgtataacca catggttacc
     8581 taatcgccaa ttaatataag ctccattatt tctccatatt tttatgttag aagtgacaag
     8641 gagaaatgta atgtttctct cagagaatat aaatcattcc ttttgcccac cttaatggtg
     8701 ggctttttta tgcaaaacct gaacgtcctg tggtattcct ctggcataat gattttcgcg
     8761 attaaccaac aaccagacca gcaaatacgc caccaccagc acagcaacat ccatcaggtt
     8821 tgccacaccc catgcgtgcg gctcaaagcc gccatggcat attaatccgc ttgccatttt
     8881 cgaacagctg ataggtttag tccggtgttt ccctgcttgc acttaaaaat gagcggatag
     8941 gaaactctcg aaatggttcg tcgatatgct cacctcgcac ctaatcacct taccgaacac
     9001 gcacggtaaa tagattcgat cctgaaccca tcggtcccaa atctgtccca atcaaaaaat
     9061 aaggaaggta ttaatgatgt gtaacttatt gatttaaaat ggataatata aacagattgt
     9121 aa
//
LOCUS       DAZFRG010000193         9023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFRG010000193 DAZFRG010000000
VERSION     DAZFRG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.194"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..496
                     /locus_tag="ACHKUC_003724"
     CDS             <1..496
                     /locus_tag="ACHKUC_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6619907.1"
                     /translation="TIPQGQGKVTFNGTVVDAPCSISQKSADQSIDFGQLSKSYLEAG
                     GVSKPIDLDIELVNCDITAFKKPSAVGGTAKAGTVKLAFTGPIVAAHSDELDTTGGTG
                     TAIVVQAAGKNITFDGVEGGANTLKDGDNVLHYTAIVKKSSVAQSISEGAFSAVANFN
                     LIYQ"
     gene            566..1153
                     /gene="papH"
                     /locus_tag="ACHKUC_003725"
     CDS             566..1153
                     /gene="papH"
                     /locus_tag="ACHKUC_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapH"
                     /protein_id="HFO6619908.1"
                     /translation="MRLRFSVPLFFFGCVFVHGVFAGPFPPPGMSLPEYWGEEHVWWD
                     GRATFHGEVVRPACTLAMEDAWQIIDMGETSVRDLQNGFSGPERKFSLRLRNCEFNSQ
                     GGNLFSDSRIRVTFDGVRGETPDKFNLSGQAKGINLQIADARGNIARAGKVMPAIPLT
                     GNEEALDYTLRIVRNGKKLEAGNYFAVLGFRVDYE"
     gene            1212..3722
                     /gene="papC"
                     /locus_tag="ACHKUC_003726"
     CDS             1212..3722
                     /gene="papC"
                     /locus_tag="ACHKUC_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HFO6619909.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPQSSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLSASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQRQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            3808..4527
                     /locus_tag="ACHKUC_003727"
     CDS             3808..4527
                     /locus_tag="ACHKUC_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6619910.1"
                     /translation="MIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGSEKSMTLDIS
                     NDNKQLPYLAQAWIENENQEKIITGPVIATPPVQRLEPGAKSMVRLSTTPDISKLPQD
                     RESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLILNKVSG
                     GYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLSYINDY
                     GGRPVLSFICNGSRCSVKKEK"
     gene            4564..5145
                     /locus_tag="ACHKUC_003728"
     CDS             4564..5145
                     /locus_tag="ACHKUC_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus-assembly fibrillin subunit"
                     /protein_id="HFO6619911.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELRH
                     YRINERGGRTGSLAISGALLSSPCTLVSNEVPLSLRPENHSAAAGAPLMLRLAGCGDG
                     GALQPGKRGVAMTVSGSLVTGPGSGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     EMLAAWRKRATQEGEYSDARSNPAMLRLSIKYE"
     gene            5155..5691
                     /locus_tag="ACHKUC_003729"
     CDS             5155..5691
                     /locus_tag="ACHKUC_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6619912.1"
                     /translation="MIKSTGALLLFAALSAGQAMASDVAFRGNLLDRPCHVSGDSLNK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNSGRLGIALLDTDGSSLLKPGTSHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            5718..6239
                     /locus_tag="ACHKUC_003730"
     CDS             5718..6239
                     /locus_tag="ACHKUC_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6619913.1"
                     /translation="MKKIRGLCLPVMLGAVLMSQHVHAVDNLTFRGKLIIPACTVQNA
                     EVNWGDIEIQKLVQSGGNQKDFTVDMNCPYSLGTMKVTITSNGQTGNSILVPNTSTAS
                     GDGLLIYLYNSNNSGIGNAVTLGSQFTPGKITGTAPARKITLYAKLGYKGNMQSLQAG
                     TFSATATLVASYS"
     gene            6314..6814
                     /gene="papF"
                     /locus_tag="ACHKUC_003731"
     CDS             6314..6814
                     /gene="papF"
                     /locus_tag="ACHKUC_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial tip protein PapF"
                     /protein_id="HFO6619914.1"
                     /translation="MIRLSLFISLLLTSVAVLADVQINIRGNVYIPPCTINNGQNIVV
                     DFGNINPEHVDNSRGEVTKTISISCTYKSGSPWIKVTGNAMAGQTNVLATNIANFGIA
                     LYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFTSVPFRNGSRTLNGGDFRTTAS
                     MSMIYN"
     gene            6858..7868
                     /gene="papG-II"
                     /locus_tag="ACHKUC_003732"
     CDS             6858..7868
                     /gene="papG-II"
                     /locus_tag="ACHKUC_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151505562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria tip G-adhesin PapG-II"
                     /protein_id="HFO6619915.1"
                     /translation="MKKWFPALLFSLCVSGESSAWNNIVFYSLGDVNSYQGGNVVITQ
                     RPQFITSWRPGIATVTWNQCNGPGFADGSWAYYREYIAWVVFPKKVMTKNGYPLFIEV
                     HNKGSWSEENTGDNDSYFFLKGYKWDERAFDTANLCQKPGEKTRLTEKFDDIIFKVAL
                     PADLPLGDYSVTIPYTSGMQRHFANYLGARFKIPYNVAKTLPRENEMLFLFKNIGGCR
                     PSAQSLEIKHGDLSINSANNHYAAQTLSVSCDVPANIRFMLLRNTTPTYSHGKKFSVG
                     LGHGWDSIVSVNGVDTGETTMRWYKAGTQNLTIGSRLYGESSKIQPGVLSGSATLLMI
                     LP"
     gene            8229..8966
                     /locus_tag="ACHKUC_003733"
     CDS             8229..8966
                     /locus_tag="ACHKUC_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO6619916.1"
                     /translation="MKKVIAVSTLAMAGMFSAQTLADENKTGFYVTGKAGVSVMSLSE
                     QRFVDGEGAWADKYKGSDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESAGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGAGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
BASE COUNT         2341 a         1904 c         2400 g         2378 t
ORIGIN      
        1 aactattcca caggggcagg gtaaagtaac ttttaacgga actgttgttg atgctccatg
       61 cagcatttct cagaaatcag ctgatcagtc tattgatttt gggcagctct caaaaagcta
      121 ccttgaagca ggaggtgtat ccaagccaat agatctggat attgagttag ttaattgtga
      181 tattactgca ttcaaaaaac caagtgctgt aggtggtact gcaaaagcag ggacggtgaa
      241 gttagctttt actggcccaa tagttgctgc ccatagtgat gagttagaca ccaccggcgg
      301 tacaggtacg gctattgtag ttcaggctgc aggtaaaaat atcacttttg atggtgtaga
      361 aggtggcgct aatactctga aagatggaga taatgtgctg cattacaccg ctattgttaa
      421 gaagtcatca gtagctcagt ccatttctga aggtgccttc tcagccgttg cgaatttcaa
      481 cctgatttat cagtaacagt aatacgataa tctggtccgg taaacagcgg aaatattccg
      541 ctgtttattt ctcagggtat ttatcatgag actgcgattc tctgttccac ttttcttttt
      601 tggctgtgtg tttgttcatg gtgtttttgc cggtccgttt cctccgcccg gcatgtccct
      661 tcctgaatac tggggagaag aacacgtatg gtgggacggc agggctactt ttcatggtga
      721 ggttgtcaga cctgcctgta ctctggcgat ggaagacgcc tggcagatta tcgatatggg
      781 ggaaacctcg gttcgggatt tacagaatgg tttctccgga cctgaaagaa aattcagcct
      841 ccggctcagg aactgtgaat ttaacagtca gggtgggaac cttttctctg attcccggat
      901 aagggtgact ttcgatggcg tccgggggga aacgccggat aagtttaatt tatccggtca
      961 ggcaaaaggc attaatctgc agatagctga tgccaggggg aatattgccc gggcagggaa
     1021 agtaatgcct gcaataccat tgacgggtaa tgaagaagcg ctggattaca ccctcagaat
     1081 tgtgcgaaac ggaaaaaaac ttgaagccgg aaattatttt gccgtgctgg gattccgggt
     1141 cgattatgag tgagtcactc cggtgagatg tccggttatt tatctttttt gtgaatctgg
     1201 tgatgcgtgt aatgaaagac agaatacctt ttgcagtcaa caatattacc tgtgtgatat
     1261 tgttgtctct gttttgtaac gcagccagtg ccgttgagtt taatacagat gtacttgacg
     1321 cggcggacaa gaaaaatatt gacttcaccc gtttttcaga agccggctat gttctgccgg
     1381 ggcaatatct tctggatgtg attgttaacg ggcaaagtat ttctcccgca tcgttacaga
     1441 tttcatttgt tgaacctcag tcgtcaggag ataaggcaga aaaaaaattg ccgcaggcct
     1501 gcctgacatc agatatggtc agactgatgg ggttaacagc agaatctctg gataaagttg
     1561 tttactggca tgatggtcag tgtgcggatt ttcatgggtt gccgggagtg gatattcgtc
     1621 ctgataccgg agcgggcgta ttacgcatca atatgccgca ggcctggctt gagtattctg
     1681 atgccacctg gctgcctcct tcacgctggg acgacggcat tcccggactg atgctggatt
     1741 ataacctcaa cgggacggtt tcccgtaatt atcagggagg agactctcat cagttcagtt
     1801 ataacgggac tgtggggggg aatctggggc cctggcgcct gcgggctgac tatcagggaa
     1861 gccaggagca gagtcgctac aacggggaaa aaacgacaaa cagaaatttc acatggagtc
     1921 gcttttatct gttccgtgcc attccccggt ggcgggcaaa cctgacgctg ggcgagaata
     1981 atatcaactc agatatattc cggtcatgga gttatacggg agccagcctg gaaagcgatg
     2041 accggatgct gccgcccaga ctgcgaggct atgcaccgca gattaccggg attgcggaga
     2101 ctaatgcccg tgttgtggtg tcgcagcagg gacgggtgct gtacgactcg atggtccccg
     2161 cagggccatt cagtattcag gacctggaca gttcagttcg cggacgtctt gatgttgagg
     2221 ttattgaaca gaatggacgg aagaaaacct ttcaggtcga tacagcctcg gttccttatc
     2281 tgacgcgtcc gggacaggtc cggtacaaac ttgtctccgg tcgttcccgt ggatacgggc
     2341 atgagaccga agggcctgta tttgcgaccg gagaggcatc ctgggggctc agtaaccagt
     2401 ggtcgctgta tggcggggct gtgcttgccg gtgattataa tgcactggca gccggtgccg
     2461 gctgggacct gggtgtgccg gggacccttt ccgctgatat cacgcagtca gtagcccgta
     2521 ttgagggaga gagaacgttt cagggaaaat cctggcgtct gagctactcc aaacggtttg
     2581 ataatgcgga tgccgacatt acgttcgccg ggtatcgttt ctcagagcga aactatatga
     2641 ccatggagca gtacctgaac gcccgctacc gtaatgatta cagcagtcgg gaaaaagaga
     2701 tgtataccgt tacgctgaat aaaaacgtgg cggactggaa cacctctttt aacctgcagt
     2761 acagccgtca gacatactgg gacatacgga aaacggacta ttatacggtg agcgtcaacc
     2821 gctactttaa tgttttcgga ctgcagggtg tggcggttgg attgtcagcc tcaaggtcta
     2881 aatatctggg gcgtgataac gattctgctt acctgcgtat atccgtgccg ctggggacgg
     2941 ggacagcgag ctacagtggc agtatgagta atgaccgtta tgtgaatatg gccggctaca
     3001 ctgacacgtt caatgacggt ctggacagct acagcctgaa cgccggcctt aacagtggcg
     3061 gtggactgac atcgcaacgt cagattaatg cctattacag tcatcgtagt ccgctggcaa
     3121 atttgtccgc gaatattgca tccctgcaga aaggatatac gtctttcggc gtcagtgctt
     3181 ccggtggggc aacaattacc ggaaaaggtg cggcgttaca tgcaggggga atgtccggtg
     3241 gaacacgtct tcttgttgac acggatggtg tgggaggtgt accggttgat ggcgggcagg
     3301 tggtgacaaa tcgctgggga acgggcgtgg tgactgacat cagcagttat taccggaata
     3361 caacctctgt tgacctgaag cgcttacctg atgatgtgga agcaacccgt tctgttgtgg
     3421 aatcggcgct gacagaaggt gccattggtt accggaaatt cagcgtgctt aaagggaaac
     3481 gtctgtttgc aatactgcgt cttgctgatg gctctcagcc cccgtttggt gccagtgtaa
     3541 ccagtgaaaa aggccgggaa ctgggcatgg tggccgacga aggccttgcc tggctgagtg
     3601 gcgtgacgcc gggggaaacc ctgtcggtaa actgggatgg aaaaatacag tgtcaggtaa
     3661 atgtaccgga gacagcaata tctgaccagc agttattgct tccctgtaca cctcagaaat
     3721 aaatgaaagt ccggaatatt aacggctgat gaatgcggtt atgccatttt ccggaccagg
     3781 tgaaattaca gaatgtggaa attaagtatg attcgaaaaa agattctgat ggctgccatc
     3841 cccctgtttg ttatatccgg ggcagacgct gctgtttcgc tggacagaac ccgcgcggtg
     3901 tttgacggga gtgagaagtc aatgacgctt gatatctcca atgataacaa acaactgccc
     3961 tatcttgctc aggcatggat agaaaatgaa aatcaggaaa aaattattac agggccggtt
     4021 attgccaccc ctccggttca gcgccttgag ccgggtgcga aaagcatggt caggctgagt
     4081 accacaccgg atatcagtaa acttcctcag gacagggaat cactgtttta ttttaatctc
     4141 cgggaaatac cgccgaggag tgaaaaggcc aatgtactgc agatagcctt acagaccaaa
     4201 ataaagcttt tttatcgccc ggcagcaatt aaaaccagac caaatgaagt atggcaggac
     4261 cagttaattc tgaacaaagt cagcggtggg tatcgtattg aaaacccaac gccctattat
     4321 gtcactgtta ttggtctggg aggaagtgaa aagcaggcag aggaaggtga gtttgaaacc
     4381 gtgatgctgt ctccccgttc agagcagaca gtaaaatcgg caaattataa taccccttat
     4441 ctgtcttata ttaacgacta tggtggtcgc ccggtactgt cgtttatctg taatggcagt
     4501 cgttgctctg tgaaaaaaga gaaataatgt accgcaataa cggttaaatg cggatgggat
     4561 attatggttg tgaataaaac aacagcagta ctgtatctta ttgcactgtc gctgagtggt
     4621 ttcatccata ctttcctgcg ggctgaagag cggggtatat atgatgacgt ctttactgca
     4681 gatgagttgc gtcattaccg gataaatgaa cgggggggac gcaccggaag cctggccatc
     4741 agtggcgcac tgctgtcctc accctgcacg ctggtgagta atgaggtgcc gttaagcctc
     4801 cggccggaaa atcactctgc ggcagccgga gcacctctga tgctgaggct ggcaggatgt
     4861 ggggacggtg gtgcacttca gcccggaaaa cggggagttg cgatgacagt ctccggctca
     4921 ctggtaaccg gtcccggaag cggaagtgct ttacttcctg accgtaagct atccggctgt
     4981 gaccatcttg ttatacacga tggtgatacc tttttactgt gccggcctga ccggcggcag
     5041 gaggagatgc tggccgcctg gaggaaaagg gcgacacagg agggtgagta ttctgatgcc
     5101 aggagcaatc cggccatgct gcgtctgagc ataaaatatg agtaagggaa tgtgatgata
     5161 aaaagcacag gcgctctttt attgtttgca gcactgtctg ccgggcaggc aatggcctca
     5221 gatgtggcat tcaggggtaa tctgcttgac agaccctgcc atgtgtccgg tgacagtctg
     5281 aataaacatg tcgtcttcaa aacccgcgcg tccagggatt tctggtatcc gcccggacgt
     5341 tcgccgacag agtcgtttgt catcaggctg gaaaactgcc atgcaacagc agttggcaaa
     5401 attgtgaccc tgacctttaa ggggacggaa gaggcggccc tcccgggcca tctgaaggta
     5461 accggagtga attccggccg gttaggcatt gcactgctgg acaccgatgg cagcagtctg
     5521 ctgaaacccg gcacctccca taacaaaggc cagggggaaa aggttaccgg aaacagtctt
     5581 gagcttcctt tcggcgcgta tgttgtggca acgccggaag ccctgcggac gaagagtgtg
     5641 gtacccggtg attatgaagc aacagccacg tttgaattaa catatcgtta gaaaaaattt
     5701 tgtacaggat atttcagatg aaaaagataa gaggtttgtg tcttccggta atgctggggg
     5761 cagtgttaat gtctcagcat gtacatgcag ttgataatct gaccttcaga ggaaaactga
     5821 ttattcctgc atgtactgta caaaatgctg aagttaactg gggtgatatt gaaattcaga
     5881 agctggtaca gagtggcgga aatcagaaag actttaccgt cgacatgaac tgcccctata
     5941 gcctgggaac catgaaagtt acaataacat caaatggtca gactggtaat tcgatactgg
     6001 tgcctaatac ttcaaccgct tctggtgatg ggctgctcat ttatctttac aatagtaata
     6061 acagtggtat tggtaacgct gtcactttag ggagtcagtt tacgcccgga aaaatcacag
     6121 gaacagcacc agccagaaag attactcttt acgcaaaact tggatataaa ggaaatatgc
     6181 agagtttgca ggcgggaaca ttttctgcaa cggcaacgct ggttgcatca tattcgtaat
     6241 actatcaatt aaaatacgtc gattttatat ctcataaaat aaaatttttt ctgtgccgct
     6301 ctccggaggg ggaatgattc gcttatcatt atttatatcg ttgcttctga catcggtcgc
     6361 tgtactggct gatgtgcaga ttaacatcag gggaaatgtt tatatccccc catgcaccat
     6421 taataacggg cagaatattg ttgttgattt tgggaatatt aatcctgagc acgtggacaa
     6481 ctcacgtggt gaagtcacaa aaaccataag catatcctgt acgtataaga gtggctcacc
     6541 ctggataaag gtcacaggta atgcaatggc tgggcagact aatgtactgg caacaaatat
     6601 agccaatttt ggtatagcgt tgtatcaggg aaaaggaatg tcaacacctc ttacattagg
     6661 taatggttca ggaaatggtt acagagtgac agcaggtctg gacacagcac gttcaacgtt
     6721 cacctttact tcagtgccct ttcgtaatgg cagcaggaca ctgaatggcg gggatttccg
     6781 gaccacggcc agtatgagca tgatttataa ctgagtcata cctaaatgaa taactgtaat
     6841 tacggaagtg atttctgatg aaaaaatggt tcccagcttt gttattttcc ttgtgtgtgt
     6901 ctggtgagtc ctctgcatgg aataatattg tcttttactc ccttggagac gttaactctt
     6961 atcagggagg gaatgtggtg attactcaaa ggccacaatt tataacttcg tggcgcccgg
     7021 gcattgctac ggtaacctgg aatcagtgta atggtcctgg gtttgctgat ggttcctggg
     7081 cttactacag ggagtatatt gcgtgggtag tattccccaa aaaggttatg accaaaaatg
     7141 gatatccctt atttattgag gttcataata aaggtagctg gagtgaggag aatactggtg
     7201 acaatgacag ctattttttt ctcaaggggt ataagtggga tgagcgggcc tttgatacag
     7261 ctaatttgtg tcagaaacca ggagaaaaaa cccgtctgac tgagaaattt gacgatatta
     7321 tttttaaagt cgccctacct gcagatcttc ctttagggga ttattctgtt acaattccat
     7381 acacttccgg catgcagcgt catttcgcga attacttggg ggcccgtttt aaaatcccat
     7441 acaatgtggc caaaactctc ccaagagaga atgaaatgtt attcttattt aagaatatcg
     7501 gcggatgccg tccttctgca cagtctctgg aaataaagca tggtgatctg tctattaata
     7561 gcgctaataa tcattatgcg gctcagactc tttctgtgtc ttgcgatgtg cctgcaaata
     7621 ttcgttttat gctgttaaga aatacaactc cgacatacag ccatggtaag aaattttcgg
     7681 ttggtctggg gcatggctgg gactccattg tttcagttaa cggggtggac acaggagaga
     7741 caacgatgag atggtacaaa gcaggtacac aaaacctgac catcggcagt cgcctctatg
     7801 gtgaatcttc aaagatacaa ccaggagtac tatctggttc agcaacgctg ctcatgatat
     7861 tgccataaat ggtttatccg gagccggata gagtgttgtg gatatctggg atgccccggg
     7921 aagtcacctt tcagccactt catttatgga ttatacgcgg cctgcactga gttacgtcca
     7981 tgtagctcat ctgacgttca gccgctttat ctcgtgctgt tctggtctca atacttccat
     8041 gccgttgcaa ttacctctta aaccattcct tatcattttc atgtcttcga taagaaatct
     8101 tcatcattca tgatcgattt tgtcgatcga ttcgatcgaa ttttctcttt ttaccttgtc
     8161 ttttgcatgg ttttaaaatg agcaaccttc tttatcgggt ggggtaagaa ccaacggaga
     8221 tgattgaaat gaaaaaggtt attgctgttt caacacttgc aatggcaggc atgttttcgg
     8281 cccagactct ggctgatgag aacaaaactg gcttttatgt gaccggtaaa gccggtgttt
     8341 ctgttatgtc actttcagag caacgtttcg ttgatggtga aggggcatgg gcggataaat
     8401 acaaaggcag tgacaaaagt gatacggttt ttggcgcagg ccttgcggtg ggatacgatt
     8461 tttatcagca ctacaatgtg ccggtgcgta ctgaagtgga gttttatggc cgcggtaacg
     8521 ctgaatcaaa atatcgtctg agttattggg aaagtgccgg tggtgccgag tttgatgatg
     8581 cacagaacaa gctcagtgta aacacgctga tgctgaatgc gtattatgat ttcagaaaca
     8641 gcagtgcatt cacaccatgg atatctgctg gtctgggtta tgcacgggtt catcataaaa
     8701 cttcgtacat atataccgat aacagcccgg caggcagtga agtttattca gcttcagcgt
     8761 caaaatacga aaacaacctg gcatggagcc tgggtgccgg tgttaaatac gatgtgacgc
     8821 aggatttcag ccttgacctc agctaccggt atctggatgc aggggattcc actctgactt
     8881 ataaagatga ggatggtgct aaatataaat cctctgttga tgtcagaagt aacgagttta
     8941 tgttaggtgc aacatataac ttctgacagc catttctgcc tgcgctttgg agcacagtaa
     9001 agtgcaggta tgacaaatca ata
//
LOCUS       DAZFRG010000194         9012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFRG010000194 DAZFRG010000000
VERSION     DAZFRG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.195"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            323..826
                     /gene="ftnB"
                     /locus_tag="ACHKUC_003734"
     CDS             323..826
                     /gene="ftnB"
                     /locus_tag="ACHKUC_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO6619917.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(905..1156)
                     /gene="yecJ"
                     /locus_tag="ACHKUC_003735"
     CDS             complement(905..1156)
                     /gene="yecJ"
                     /locus_tag="ACHKUC_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO6619918.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1271..1357)
                     /gene="azuC"
                     /locus_tag="ACHKUC_003736"
     CDS             complement(1271..1357)
                     /gene="azuC"
                     /locus_tag="ACHKUC_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO6619919.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1621..1944
                     /gene="yecR"
                     /locus_tag="ACHKUC_003737"
     CDS             1621..1944
                     /gene="yecR"
                     /locus_tag="ACHKUC_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO6619920.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2116..2613
                     /gene="ftnA"
                     /locus_tag="ACHKUC_003738"
     CDS             2116..2613
                     /gene="ftnA"
                     /locus_tag="ACHKUC_003738"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO6619921.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2651..2890)
                     /gene="yecH"
                     /locus_tag="ACHKUC_003739"
     CDS             complement(2651..2890)
                     /gene="yecH"
                     /locus_tag="ACHKUC_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO6619922.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            3081..4292
                     /gene="tyrP"
                     /locus_tag="ACHKUC_003740"
     CDS             3081..4292
                     /gene="tyrP"
                     /locus_tag="ACHKUC_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO6619923.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQVGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4354..5019)
                     /gene="yecA"
                     /locus_tag="ACHKUC_003741"
     CDS             complement(4354..5019)
                     /gene="yecA"
                     /locus_tag="ACHKUC_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO6619924.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5215..5301)
                     /locus_tag="ACHKUC_003742"
     tRNA            complement(5215..5301)
                     /locus_tag="ACHKUC_003742"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5265..5267),aa:Leu,seq:taa)
     gene            complement(5314..5387)
                     /locus_tag="ACHKUC_003743"
     tRNA            complement(5314..5387)
                     /locus_tag="ACHKUC_003743"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5353..5355),aa:Cys,seq:gca)
     gene            complement(5441..5516)
                     /locus_tag="ACHKUC_003744"
     tRNA            complement(5441..5516)
                     /locus_tag="ACHKUC_003744"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5481..5483),aa:Gly,seq:gcc)
     gene            complement(5668..6216)
                     /gene="pgsA"
                     /locus_tag="ACHKUC_003745"
     CDS             complement(5668..6216)
                     /gene="pgsA"
                     /locus_tag="ACHKUC_003745"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO6619925.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAL
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGITLFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6273..8105)
                     /gene="uvrC"
                     /locus_tag="ACHKUC_003746"
     CDS             complement(6273..8105)
                     /gene="uvrC"
                     /locus_tag="ACHKUC_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO6619926.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8102..8758)
                     /gene="uvrY"
                     /locus_tag="ACHKUC_003747"
     CDS             complement(8102..8758)
                     /gene="uvrY"
                     /locus_tag="ACHKUC_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO6619927.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2258 a         2266 c         2065 g         2423 t
ORIGIN      
        1 aaaatgaaaa aacgttttat aaaatcattg cttcatcatg aattcaaatt catcgattaa
       61 tatcaacaag atacaaaaag caccatcatt aaaattcatt acagttacat tgatttcatc
      121 aatgaaatgt aaaaatatat aaacttgatg atttaagcat tttcttatac ccgttcagac
      181 gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag
      241 aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact
      301 tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca
      361 aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga
      421 acaaagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca
      481 aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat
      541 tgatgttccc ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga
      601 atacgagcaa cgttcaagca cgctggcgca gttagccgat gaagcgaaag aactgaatga
      661 tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggcctgtt
      721 actgcaaacc attcttgatg aagtgcgcag tgcgaaactt gctggtatgt gtcctgtgca
      781 gaccgaccaa catgttctga atgtcgtgtc gcatcagctg cattgatcat acatcggcgc
      841 taatgcattg cgccgatgaa gtttttgaga aaccgctgcc tcattttttt gaagcagcgg
      901 ttttttaatg ggattcgccc tgtggggtaa acttgagttc aatcaacgcg atggcttttt
      961 ggattgcccg catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct
     1021 tcagctggct ggacattttt tcgcgaactt caacgggcgc gatcacatcg agaacatcca
     1081 gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc tgatcggcat
     1141 tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct
     1201 tcacatcttc agcgatagtc acatccaccg tcatcagaac acaaaaaaac ctgccggagc
     1261 aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt
     1321 tattgaacat acttttcagg atcttgcgca gtttcatcgc ggtactccga ccatttgtta
     1381 tacaggtgtt attgtctttg cgtttataat atgacaacca tcacaaaaat caatctttat
     1441 gtgatgtaaa tcacataaat acccctttaa tgttataaaa atgataatca aaaagcagcc
     1501 cccccaattt ctgacacata tagatggcaa gaaatagcgc ctgccaggcg tcttttccgg
     1561 ccattgtcgc agcgctgtaa cgcgtaaaat actgctttct tttactcttc tggctggacc
     1621 atgagacttc tgattctgac tctttcctta ataacgctga caggatgtac ggttactcgc
     1681 caggcccatg tgagcgaagt tgacgcggca acaggtattg ttcggttggt ttatgatcag
     1741 gcttttttgc agcatgcccg taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca
     1801 tgccagcagg caggctatac ccacgctatc ccctttggtc agccagtagg caactgtagc
     1861 ctctttgcgg gttccttatg tttgaatacg gaattcactt tgtcatatca gtgccatcat
     1921 tccgccttcc ctgtctttct ctaaacgttt taatcagcga gggggatctt cgctgattaa
     1981 ataaatagcg gtaatgcgtt ttattcccat tcgtattttt aataaatgat gtttatattt
     2041 taccttttgc aaataataaa ataacaaatt atagtgacgc tacagcaaca aatataacct
     2101 ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac
     2161 ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc
     2221 ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac gcatatgcag
     2281 cgtctgtttg attacctgac tgataccggc aacttaccgc gtattaatac cgttgaatct
     2341 ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa
     2401 ttaatcacgc agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca
     2461 acatttaatt tcctgcaatg gtatgtttct gagcagcatg aagaagagaa actgttcaaa
     2521 tcgattattg ataaattaag cctggcaggc aaaagtggcg aaggtctgta ttttatcgac
     2581 aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg ttttctgccg
     2641 ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt tgaaaaacct tcttctgaag
     2701 gtataaattt gccttttgct gccagaaacg ccaccaactc tcccgcagtc atcccttctg
     2761 ccgagcaggt gtgaaaacgt gcctgttcac caaaacgcgc tttaattgct gcttccagac
     2821 tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca tgaccgtgaa
     2881 tagagtccat cgttcctcct caaaaaaagc ctagcgtagc gattgccgct tatgaagact
     2941 ttgcgccagc gcagaactga acgctttttg ttgtacattt atatttacat catatgtaac
     3001 gtcggtttga cgaatcagcc gttatgcctt aacctgcgcc gcagatatca ctcataaaga
     3061 tcgtcaggac agaagaaagc gtgaaaaaca gaactctggg aagtgttttt atcgtggcgg
     3121 gaaccacaat tggcgcaggc atgctggcaa tgccgctggc tgcggcaggt gttggtttta
     3181 gcgtcacgtt aatcttgttg attgggcttt gggcgttgat gtgctacacg gcgctattac
     3241 tgctggaggt gtaccagcat gtcccggcag ataccggtct gggcacgctg gcaaaacgct
     3301 atctgggacg ctacggtcag tggctgacgg gcttcagtat gatgttttta atgtatgctc
     3361 tgactgcggc atacatcagc ggtgccggtg aattgttggc ctccagcatc agcgactgga
     3421 caggtatttc aatgtcggca accgctggcg tgctgttgtt cacttttgtt gccggtggcg
     3481 tggtttgtgt cggaacttcg ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa
     3541 ttatttttct ggtggtgatg ctggtattgc tgctaccgca tattcacaaa gtgaatcttt
     3601 taaccctgcc gttgcaacag ggactggcac tgtctgcaat cccggtgatt tttacgtcgt
     3661 ttggttttca cggtagcgtg ccgagtattg tcagctatat ggatggcaac attcgtaagc
     3721 tacgctgggt gtttataatc ggtagtgcga tccccctggt ggcatatatt ttctggcagc
     3781 tggcgaccct tggcagcatt gattcaacaa cctttatggg attgctggct aatcatgccg
     3841 gattaaacgg gctgttacag gcgttacgcg aaatggtggc ctctccgcat gttgagctgg
     3901 cagtgcattt atttgctgat ttagccctcg ccacgtcctt cctcggcgta gcgttaggct
     3961 tatttgatta tctggcagat ttgtttcagc gttcaaatac cgttggtgga cgattgcaaa
     4021 ctggtgcaat tacctttctg ccgccactgg cgtttgcact gttttatcca cgaggatttg
     4081 tgatggcgct gggttacgcc ggtgtggcac tggcggtact ggcattgatt atcccttcgc
     4141 tgttgacctg gcaaagcagg aagcacaatc ctcaggtggg ttaccgggtg aaaggtggtc
     4201 gtccggcgct ggtggtggtg tttctctgtg gtattgctgt gattggcgtg caatttttga
     4261 ttgcggcagg cttgttacca gaagtggggt gattagatag cctcaaattc cttattgggt
     4321 gccagaatta acgctgacac ccaatgtgac ctcttaatgc aggcagcact gcttaaattt
     4381 cttaccacta ccgcacggac aaggatcgtt acgccccggt ttctcttctg ctttgatagg
     4441 ttgctgaaca gctttttcct gcggatgcgc catccagtat gcatgtaaat caagcgccgc
     4501 cagtcgaata gcatctacgc tctcttcaaa cgcttctggc gacatcttct ctacccgctc
     4561 gaagttttcc tcagtaccgt gcagcgcaat cgcatccagc gctggtttta acgaatcggg
     4621 caacgttgac cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac
     4681 aatcgtcagc tcgctgccat cgacttctcg caagccgaat aacggctcaa actgctccgg
     4741 gaattcgttc agacgctctg cggtatcagc catatgttga aaagccaggt tcataaagcg
     4801 cgtcatctct ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac
     4861 cagccactgt gccggttcaa tctcttgcgg agaactcaac accgccgtca ataaaccgtc
     4921 cagctccgcc acatcaagaa tggcgtggtc agtgttgtat ttggtcagaa tatcgtccag
     4981 ccattccaac tcactttcgt ttaacggtcc tgttttcata cgcttttcct tgtggatctc
     5041 aactcgccag cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt
     5101 gccggtgatt aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc
     5161 gaaggtggtt tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac
     5221 ccggagcggg acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc
     5281 taccgattcc accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg
     5341 actagacgga tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc
     5401 ctttcagcca gacatccgct gccgccgatg tcttttaaac tggagcggga aacgagactc
     5461 gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat tcccgcactc
     5521 atcaagcaat cagttaatca cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg
     5581 atgcgttgca ttctacttac ctggcgcgat gagtcaacga tatttttcac cacttttgat
     5641 cgtttgctga aaattgcgcc gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct
     5701 caaatattgc aacattgacc acagagtcag taccgcagcc acaaagaaaa gtgtaatacc
     5761 ggcgtactca acccaaatgt tcggacgcca cagcagccag gctaacgcca ccatctgggc
     5821 agtggttttc actttcccaa tccaggagac agccacgcta ctgcgtttac ccaactccgc
     5881 catccattcg cgtagcgcag aaataataat ttcacgggcg atcatcgttg ctgccggtaa
     5941 ggtcacccac cagctgtggt aatgctcggt taccagcacc atggcgatag ccacgagaac
     6001 tttatctgcg acagggtcaa ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc
     6061 cagaaaacca tcgaaccagt cagtcagcgc cgcgacgcag aaaatgagcg cggcggcaaa
     6121 tggcgaccag gtgacaggca aataaaagac caatacaaag aatgggataa ggatgacacg
     6181 gaacagtgta agcaacgtag ggatattaaa ttgcataatg acgggtaact atctgttgtc
     6241 agtaagatta cccctatgtt gctacagaga catcaatgtt tcaacgacca gaagatcttt
     6301 tctgccagac cttgcgaaat acccggcact tttgcaattt cctcgacgct ggcgttacgt
     6361 aaaccttgca aaccgcccat atatttcaac aacatttgcc gacgttttgg cccgacgcct
     6421 tcaatggttt ccagggaact ggtatttttg accttcgccc gttttttacg gtgcccgcca
     6481 atcgcgtgat cgtgtgattc atcgcgaata tgctggataa catgcagcgc gggagaatct
     6541 ggcggcaaac taaatccctc accttccggc tcaaagaaca gcgtttccag cccagcctta
     6601 cgatctgctc ctttggcaac accaagtagc agcggatgat ttttatccca tgagacatcc
     6661 agttcggcga aaacattttt cgcctgcgca agctggcctt tgccgccgtc aataaggatc
     6721 acatccggga tcttactgtc gtcgatagct ttaccataac gccgacgcag cacctgattc
     6781 atcgccgcgt aatcatcgcc cggcgtgatg ccagtaatgt tatagcgccg atactccgca
     6841 cgcagcgggc cgttggcatc aaacaccaca caggaagcga cggtttgttc acccatggta
     6901 tggctgatgt caaagcactc catccgcttc acttccggca atttcaacac gctggcaagc
     6961 gcggtcagcc gctggtgaac ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc
     7021 gcattggtac gcgcgagttt cagataacgc gctctatcgc cacgaggttt ggtttgaaca
     7081 ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga
     7141 ttaaaatcga gcaggatctc acccggtaag gtgcgcatct ggctgccttg taaatagaac
     7201 tgacctacga aggtttccac cacctcgctc agttccgtac caccaggcac tttcgggaaa
     7261 tagctgcggc tgccgagcac tttgccctga cgaatgaaca atacgtggac acaagccata
     7321 cccgcatcga acgccactcc aataacgtca aggtcgtcgc cggtattgga aacaaactgt
     7381 ttttcggtga cacgtcgcac cgcctgaatt tggtcgcgaa tccgcgccgc ttcttcaaac
     7441 tccagattct ggctggcagt ttccatacga ctaatgagtt gcgtaagcac ctgatcatct
     7501 ttgccagaca aaaacaagcg cacatactcg acctgctgag cgtattcttc ttcactcacc
     7561 agtccttcaa cgcacggccc cagacagcgc cctatctgat attgcagaca cggacgcgag
     7621 cgattgcgat aaacactgtt ttcgcactgg cgaatgggaa aaatcttttg cagtagcgcc
     7681 agtgtttcac gtacggcata gccattcggg aacgggccga aatattcacc tttggcatgc
     7741 ttcgctccac gatgcatcgc cagacgcggg tgggtatcgc cactcaggaa gataaaagga
     7801 tatgatttat catcgcgtag caaaacgttg taacgcggct gataaagttt gatgtagttg
     7861 tgttccagta acagcgcttc ggtttctgta tgagtaaccg ttacatcaat ttgctggatc
     7921 tgggcgacca gcgcttcggt tttgcgcgaa gcgaggttgc tacggaaata gctggaaagc
     7981 cgttttttca ggtctttcgc tttgccgaca tagataaccg taccaccagc atcgtacatg
     8041 cgataaacgc ctggctggct ggttacggtt tttaaaaacg cttttgcgtc aaactgatca
     8101 ctcactgact tgataatgtc tccgcattac acagaccatg gcgaattgcc aggtgagtca
     8161 gctcaacatc gccatgaatg tttagtttac tgaacatacg atagcggtag ctgttcaccg
     8221 ttttcggact gagattgagc tgttctgaga tctcattgac cttctggccc ttggtgatca
     8281 tcagcataat ctgcaattca cgttcagaca aactggcaaa tgggctttct gttttttctg
     8341 gttcgatctg gcttaacgcc atttgttgag cgatatcaga agcaatgtaa cgctgccctg
     8401 aatagacaga acgaatcgca ctaacgactt cctgcggagc cgcgcctttg ctgaggtagc
     8461 ccgcagctcc ggcctgcatg actttcgctg gtaaagggtt ttctgtatgg acggtaagca
     8521 tgatgatttt gacatcagct gtggaacgcg cgattttacg cgtcgcctca agaccgccaa
     8581 tgcccggcat actcatgtcc attagcacca cgtcaacggc atttgcccgg caccatttaa
     8641 cggcgtcttc accgcacgat gcctcaccga cgacttttat accctttata tcttccagaa
     8701 tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac aagtagaacg ttgatcaaag
     8761 gaatatctcc agaaataggg ataacgctac tgatagttag tcattcgtat attagcggtt
     8821 tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg atttttctct cgtttttgtc
     8881 aattcgattg tccacagtta tggtcacgcc cgtcaccaac ggcttccggg atcgtttcac
     8941 agtaagcccg gcgatgaaat gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag
     9001 cccgctgaat at
//
LOCUS       DAZFRG010000195         8980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFRG010000195 DAZFRG010000000
VERSION     DAZFRG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.196"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..540)
                     /locus_tag="ACHKUC_003748"
     CDS             complement(<1..540)
                     /locus_tag="ACHKUC_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6619928.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQD"
     gene            1018..1806
                     /gene="fabI"
                     /locus_tag="ACHKUC_003749"
     CDS             1018..1806
                     /gene="fabI"
                     /locus_tag="ACHKUC_003749"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO6619929.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1951..3078
                     /locus_tag="ACHKUC_003750"
     CDS             1951..3078
                     /locus_tag="ACHKUC_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO6619930.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIINNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3146..5080
                     /gene="rnb"
                     /locus_tag="ACHKUC_003751"
     CDS             3146..5080
                     /gene="rnb"
                     /locus_tag="ACHKUC_003751"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO6619931.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5157..5260)
                     /locus_tag="ACHKUC_003752"
                     /pseudo
     CDS             complement(5157..5260)
                     /locus_tag="ACHKUC_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5314..7299
                     /gene="pdeR"
                     /locus_tag="ACHKUC_003753"
     CDS             5314..7299
                     /gene="pdeR"
                     /locus_tag="ACHKUC_003753"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6619932.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRT"
     gene            complement(7281..7409)
                     /gene="ymiD"
                     /locus_tag="ACHKUC_003754"
     CDS             complement(7281..7409)
                     /gene="ymiD"
                     /locus_tag="ACHKUC_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO6619933.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYVRFR"
     gene            7447..7620
                     /gene="yciZ"
                     /locus_tag="ACHKUC_003755"
     CDS             7447..7620
                     /gene="yciZ"
                     /locus_tag="ACHKUC_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO6619934.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7710..8459
                     /locus_tag="ACHKUC_003756"
     CDS             7710..8459
                     /locus_tag="ACHKUC_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO6619935.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            8728..8946
                     /gene="osmB"
                     /locus_tag="ACHKUC_003757"
     CDS             8728..8946
                     /gene="osmB"
                     /locus_tag="ACHKUC_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO6619936.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2240 a         2231 c         2172 g         2337 t
ORIGIN      
        1 atcctgatat atttcctgca gatgttgtgg atcgcttttt tgcggtgtta aacgacgata
       61 aaccgtacct tccatcatgg tagcaaacac ttcaacgcag caacgaatgt gctcgtcgct
      121 aagatgggga tgcattcttt tcatatgagc acaaccgtta gcgaacattc tggcgtcagc
      181 ttcttcaagc attgccatca cccgcgggtt gcgtgtggct tctgcagcca cttccagcat
      241 cagcgcgtcg tcgtcctcat ttaaggattt acgcaaggca agaacttccg gaaagtgatc
      301 ggtacgcgcg tcaatgtcca tttgggcaat acgaaagtcg atgatacggc gaaccatctc
      361 ttcaataatg gcgtctttgt tggcgaagta acgatatatc tgacccacgc taagctgagc
      421 ttcgctggca atttgcgaca tactggcggc atgaaatccg cagcgacgaa aacaccgacg
      481 ggcggcgtta atgatttcat cctgacgctg tttgtggcgt atctccagct tgctggtcat
      541 ttctgtctcc tggcctatca cttggtgaga atgatcgttc tcatttttgc tgattatacg
      601 tttctccaaa gtaatggaaa gtatttttat cttattcatg gtgcatattc tgataatgaa
      661 gagattgtgg agattctgtg aagatagttg acaatattaa gttggggtca attcatcaga
      721 aaggcagcga cttgttgtcg ccagttctgt tttcatcatc aaacttatag gagctaatac
      781 gaatagtttc aaagtcagat tctataagat atttccccaa cctccccctt gcaggcgtca
      841 ggagtagata acgcaaaatg ctctgtaccg cagcttctct ccgctacaga aagcgcaact
      901 atagccaccc acagccaggt tgattataat aaccgtttat ctgttcgtac tgtttactaa
      961 aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg
     1021 ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact gtccatcgcc
     1081 tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac
     1141 gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga catcgttctg
     1201 cagtgcgatg ttgccgaaga taccagcatc gacaccatgt tcgctgaact ggggaaagtt
     1261 tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat
     1321 ggtgactatg ttaacgccgt tacccgtgaa ggcttcaaaa ttgctcacga catcagctcc
     1381 tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg
     1441 ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg
     1501 gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt
     1561 gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcttc cggtatcaaa
     1621 gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact
     1681 attgaagatg tgggtaactc cgcggcattc ctgtgctccg atctctctgc cggtatctcc
     1741 ggtgaagtgg tccacgttga cggcggtttc agcatcgctg caatgaacga actcgaactg
     1801 aaataatcgt tctgttggta aatattgggc ggcgttctgc cgcccgttat ctctgttata
     1861 cctttctgat atttgttatc gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat
     1921 aacgatttcc tttacgacca aggagcgccc atggaacaac gccacatcac cggcaaaagc
     1981 cactggtatc atgaaacgca atccagtact gcggagtatg acgttctgcc tctggtcccg
     2041 gaagccgcaa aggtcagcga tccctttctg ctcgacgtga tccttgatga agaaacgctg
     2101 gcacccttcc tttcatggct ggtccctgcg cgcgttcttg cagtagagtt gttccctgac
     2161 cagcttaccg tgacccgttc acagaccttc accgcttatg aacgcttgtc gacggccctg
     2221 acggttgctc aggtttgcgg cgtccaacgg ttatgtaact actattcggc gcgacttacg
     2281 ccgctccccg ggcctgattc ctccagggaa agtaatcatc ggttggcaca aatcacgcaa
     2341 tatgcccgcc aactggctag ctcgccttct attatcaaca accgatcgcg ccagcatctg
     2401 aatgacgttg gtcttactgt ctgtgactgt gtgatcatta accaaatcat tggttttatt
     2461 ggctttcagg cgcggacaat tgcgacattt caggcttatc tcgggcatcc ggtacgctgg
     2521 ttacccggtc tggagataca aaactacgcc gacgcgtcac tgtttgctga tgaatcaata
     2581 cgctggcgaa gcagctatga ggtggaaaaa ctccctgaag agcacacaaa aagttcaact
     2641 gcagaacttt gccaactggc caacacactc tctctccacc ctatttcact ttcccttctc
     2701 gaaaagttgt taaacagcac acgggtcaat acacagccgg ataatcagct tgcggcgttg
     2761 ttatgcgcgc ggataaatgg cagtcctgct tgttttgccg cctgtatgaa ttcatcaaat
     2821 gaatataaaa aaatcagccc ccttctgcgc aagggcgaaa atgaaattaa ccgatgggct
     2881 gaccgtcatt ctgttgagcg cgctaccgtt caggcgatac aatggctgac ccgagcaccc
     2941 gatcgcttta gcgccgccca gttcagccca ttactcgaac acgaaaaatc atcaacgcag
     3001 attattaatc tgctggtatg gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg
     3061 ttgggtgaga catattaacc ttgccgcgtc agacagattc gcgtaaaact gtcagccgct
     3121 ctaatggcca ccaaaataga caattatgtt tcaggacaac ccgctgctag cgcagcttaa
     3181 acagcaactg cattcccaga cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg
     3241 ctttggcttc ctggaagtcg acgcgcaaaa aagttatttc attccgccgc cgcagatgaa
     3301 aaaagtcatg catggcgacc gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc
     3361 cgcagagcca gaagaactgg ttgaaccgtt cctgactcgt ttcgtgggta aagttcaggg
     3421 caaaaatgac cgtctggcca tcgttcctga tcatccactc ttaaaagacg ccattccttg
     3481 ccgcgcagcc cgtggcctga accacgagtt taaagaaggc gactgggcgg ttgccgaaat
     3541 gcgccgtcat ccgctgaaag gcgatcgttc tttctatgct gaactgacac aatacatcac
     3601 ttttggtgac gatcactttg taccgtggtg ggttaccctt gcgcgtcata atctggaaaa
     3661 agaagcacca gacggcgtcg ctaccgaaat gctcgatgaa ggtctggttc gtgaagatct
     3721 gactgcgctg gattttgtca ccatcgacag tgccagcaca gaagatatgg atgacgccct
     3781 tttcgccaag gcgttaccgg atggcaaact tcagctgatt gtggcgattg ccgatccaac
     3841 cgcgtggatt gctgaaggca gcaagctgga caaagccgcg aaaattcgcg cattcaccaa
     3901 ctatctgcct ggcttcaaca tccctatgct gcctcgcgag ctttctgacg atctctgctc
     3961 actgcgcgcc aatgaagtcc gcccggtact ggcatgccgc atgacgctct ccactgatgg
     4021 caccattgaa gataatatcg aattctttgc cgccaccatc gaatccaaag cgaagctggt
     4081 gtatgaccag gtttctgact ggctggaaaa taccggtgac tggcagcctg aaagcgaagc
     4141 aattgccgaa caagtccgtt tgctagcaca aatttgccaa cgccgcggcg agtggcgtca
     4201 taaccacgca ctggtgttta aagatcgccc ggattaccgc tttattctcg gtgaaaaagg
     4261 tgaagtgctg gatatcgtcg ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc
     4321 gatgattgcc gctaacattt gtgcggcccg cgtactgcgc gataagctcg gttttggcat
     4381 ctataacgtg catatgggct ttgatccggc gaatgccgac gcgctggcag cgttgctgaa
     4441 aacgcacggt ctgcatgtcg atgctgaaga agtgctcacg ctggacggtt tctgcaaact
     4501 gcgtcgtgaa ctggatgcgc aaccaactgg tttcctcgac agccgcattc gtcgcttcca
     4561 gtcatttgct gaaattagca ctgaacccgg tcctcacttt ggcctcggtc tggaagcata
     4621 cgccacctgg acttcgccga tccgtaaata tggcgacatg atcaaccacc gtctgctgaa
     4681 agcggttatc aaaggcgaaa ctgcgacgcg tccacaggat gaaatcactg tccaaatggc
     4741 cgagcgtcgc cgtctcaacc ggatggcaga acgtgatgtt ggcgactggt tatacgcacg
     4801 cttcctgaaa gacaaagccg ggaccgacac ccgtttcgcg gcggaaattg tcgatatcag
     4861 ccgtggcggc atgcgtgttc gtttggttga taacggcgct atcgccttta ttccagcacc
     4921 tttcttacac gctgtgcgcg atgaactggt ttgcagccag gaaaacggca ctgtacaaat
     4981 taaaggtgaa acggcttaca aagtgactga cgttattgac gtcaccattg ccgaagtccg
     5041 catggaaacc cgcagcatta ttgcgcgccc agtcgcgtaa tctcctttca cggcccattc
     5101 ctcatggatg ggccgtttat ttccccgctc aaccttcgtc atatcccggc agatttttaa
     5161 ctgactttcg tttgaaaact ggcgtttttc caaaactgga ttacaaataa atatataaaa
     5221 aaacaatgtg tttagccgcc gttaatttca tgtaagtcat gttgttcctt ttatttaact
     5281 gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga gtccacaacg
     5341 ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag cagcgatgtt
     5401 ttgcgctttt ctaccaccga aatcacagaa cccgatcgta ttttgcaact atctgccgaa
     5461 caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat gagtctgacc
     5521 gtcgatgaaa gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa acgagaatgg
     5581 ggaggcaacg cgtctgcatg gcatgacaca ccggcggttg ctcgtgattt atcacatggg
     5641 ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat tctcgacagc
     5701 cgggggaata tccagcgctt taatcggtta tgtgaagatt acacaggctt gaaagaacac
     5761 gacgtcattg ggcaaagcgt gtttaaactg tttatgagtc gtcgtgaagc tgcggcatcc
     5821 aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga actgtggata
     5881 ccaacacgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca cagcggcagt
     5941 ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga gcgccgcgct
     6001 caggagcgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc gaatcgtaac
     6061 gcaatgcagg agttaatcga tcacgctatt aatcaggcag ataacaataa agttggggtt
     6121 gtgtatcttg atttggataa ttttaaaaag gtcaacgacg cctatgggca tttgtttggt
     6181 gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca tgaccaggtg
     6241 ttggcgcgtc caggtggcga tgagtttctg gtactggcat ccaatacctc acaaagcgcg
     6301 ctggaagcat tggcatcacg aattttgacc cgcttacgac tcccctttcg cattggttta
     6361 attgaagttt ataccagctg ttcagtaggt atttcactct ctcccgaaca tggttcggac
     6421 agcgcggcta ttattcgtca cgccgacaca gcaatgtaca cagcgaagga aggcggacga
     6481 ggacaatttt gcgtttttac cccagaaatg aatcagcggg tatttgaata tctctggctg
     6541 gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta tcaaccgaaa
     6601 atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca gtcacctgaa
     6661 cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagaatcagg gctaattgtg
     6721 cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tagcgaagtg gcgggataaa
     6781 ggcattaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga tcaaaccatt
     6841 ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc tatagatgtt
     6901 gaactgacgg agagctgcct gattgagaat gatgaactgg cactgtctgt tattcaacaa
     6961 tttagccgat taggtgcgca agttcatctg gacgattttg gtaccggcta ctcttcactt
     7021 tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt tgttcgggat
     7081 attcacaaac agcctgtctc gcaatcactg gtccgggcga tcgtcgctgt ggcccaggca
     7141 ttgaatcttc aggtgatcgc cgaaggtgta gaaagtgcaa aggaagatgc ttttttaacc
     7201 aagaacggga tcaatgagcg acaaggattt ttgtttgcca aaccgatgcc cgccgttgcc
     7261 ttcgaacgct ggtataaacg ctacctgaag cgcacataag tagatgaata tatatactta
     7321 gcgctatatt ccgtcattat atatcgtatt cccggatgat aattagccgt tgctgttaaa
     7381 cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc tataaggatg
     7441 tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat tgtgttagat
     7501 atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact taaagcagaa
     7561 cttttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg ggagatttaa
     7621 caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat ttttttattt
     7681 agtattataa cgttataaga attacagcga tgaattcccg acaacaaact attctacaga
     7741 tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact ggagtttctg
     7801 aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc cgacgtgcac
     7861 atggttttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg agcaactata
     7921 cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc gaaaccatct
     7981 ttatcgaaaa tggtagcagc aatgccctgc tcgccaggac tctgggcgag cagaagaaaa
     8041 atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac gcgccttgtg
     8101 aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt ggccctttga
     8161 cacgccagtg catccaacaa gtgcatttca gcaaagcatt tattggtatt gatggctggc
     8221 aacctgaaac tgggtttacc ggtcgcgata tgatgcgtac cgatgtggtc aatgccgtgc
     8281 tggaaaaaga gtgcgaggcg atagtcctga ctgacagctc gaaatttggt gctgtacatt
     8341 catactccat cggccccgtt gagcgattca atcgtgtgat taccgattcg aaaatacgcg
     8401 ccagcgatct gatgcatctt gaacaaagta aactcaccgt tcacgtcgtt gacatttaac
     8461 catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta agatgattcc
     8521 tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa aattattatc
     8581 agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc gagcggattt
     8641 cacggaataa tttcaccagg cttattctta gctattatag ttatagagag cttacttccg
     8701 tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat gaccgcggct
     8761 gttctggcaa ttaccctggc aatgtctctg agtgcctgtt ctaactggtc taaacgggac
     8821 cgcaacaccg caatcggcgc aggtgcaggg gcattaggcg gtgcagtact gaccgatggc
     8881 agtacgttgg gtacattagg tggtgcagcc gtcggtggtg ttattggtca ccaggtcggt
     8941 aaataatcgt aaataaatgt cgattatcga ctcgcaatta
//
LOCUS       DAZFRG010000196         8958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFRG010000196 DAZFRG010000000
VERSION     DAZFRG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.197"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(145..2412)
                     /gene="adiA"
                     /locus_tag="ACHKUC_003758"
     CDS             complement(145..2412)
                     /gene="adiA"
                     /locus_tag="ACHKUC_003758"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO6619937.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKVGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2611..3543)
                     /gene="melR"
                     /locus_tag="ACHKUC_003759"
     CDS             complement(2611..3543)
                     /gene="melR"
                     /locus_tag="ACHKUC_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO6619938.1"
                     /translation="MFQERESSMNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPH
                     IMPTSHWHGQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMA
                     IFNLPMHLFLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQL
                     AIDEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTI
                     NDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGF
                     RSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3802..5157
                     /gene="melA"
                     /locus_tag="ACHKUC_003760"
     CDS             3802..5157
                     /gene="melA"
                     /locus_tag="ACHKUC_003760"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO6619939.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5154..5804)
                     /gene="yjdF"
                     /locus_tag="ACHKUC_003761"
     CDS             complement(5154..5804)
                     /gene="yjdF"
                     /locus_tag="ACHKUC_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO6619940.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(5927..7573)
                     /gene="fumB"
                     /locus_tag="ACHKUC_003762"
     CDS             complement(5927..7573)
                     /gene="fumB"
                     /locus_tag="ACHKUC_003762"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6619941.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7651..>8958)
                     /gene="dcuB"
                     /locus_tag="ACHKUC_003763"
     CDS             complement(7651..>8958)
                     /gene="dcuB"
                     /locus_tag="ACHKUC_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO6619942.1"
                     /translation="ICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVI
                     IAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTI
                     LPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNVTFDGRHLEF
                     LDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDK
                     KLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIIL
                     TKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLGEMVKEYPWAYA
                     IVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQ
                     FDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2112 a         2252 c         2427 g         2167 t
ORIGIN      
        1 aaaaaaaagc gcggggtggg accgcgcaaa aagcatgaca ttttttgttt gcagcaattt
       61 ttatgaaaac gttgccatac ttaaaaggcc tcaagccagg cggcgtaatg ttatttaaca
      121 attacgcctt cagcggaata gtggttacgc tttcacacac ataacgtggt aaataccgtc
      181 aataatttct gtcccttctg tttcgtgttc aaatccaggg aaatgatggt cccaggattg
      241 cagtgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt caccagacag
      301 cagcatcggg attcctggcg gatacgggat aactgagttt gccgcgatac gtcctggcag
      361 attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg
      421 ggtgatttcc gccaccggta ggccagaata ggcttcattc aaccgtgcgc cagggttgtt
      481 ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt
      541 gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt tggcatcata
      601 gtggcgtttg aaggagcaaa gggtgttaac cagagtcccc catttcccac gggtcacgcc
      661 catagagaac aggaacataa tctggaagtc agtggtacgg gtaggcacaa tgccgtggcg
      721 accaagccag gctgtgacca gcgccgccgg aacaccggtt tcttccagtt caccatcttc
      781 acccattccc ggagcaagga tgctgacttt aatcgggtcg agcatactcc agttatccgg
      841 aatatctttg aagccgtgcc agctttcgcc cggatgcatg acccagcagt cctgaacggt
      901 ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt
      961 gacgacttct ttgttccatg gtttgaagaa ccagctaccg tcagcggtga actctttata
     1021 tagccgcgcc atcgcctgac ggaaatcaac cgcttcgtcg atcacttcct gggtcagtga
     1081 caggccgctg ttgccgtcca tcatcgacac tgccacgtcg ttggatgcgc agatggcata
     1141 cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga agttaatcgc
     1201 cccacgacct tcacgtacat gaatataaga ggcctgggac agcgcattca gcagtttgtg
     1261 ggtggagtgg gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc
     1321 atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg
     1381 cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat aacacacgcc
     1441 gtcataggta cagttggtca ccacgcagta agacggtttt tgcccgactt tgtctttggt
     1501 cagcgggctt tcactgattt tcttctgcaa ggtttcaggt tgcatttcct gcggatagat
     1561 tggcccgata atgccgtagc ggttgcggct tggcaccatg tagaccggtt tcgcgcctgt
     1621 cagcatcaag ccttgttcga tggatttatg gcagttacgg tcaacgacca cgacatcgtt
     1681 atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga cgactaccga
     1741 ccaggagcga tcggcaccaa atacgcgtgc ggcatatctt tcgctttcgc caaatgcgcc
     1801 agtatggtca agcaaagaac cgagggaagt gcgttcgatg cccatgtcgg tgcggaacag
     1861 attttcacca taatagtcat ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc
     1921 gccctggtgg cctggcgctg cccaggaata ttcatggatg tcgctatatt tcatcagcgc
     1981 gctgaacagt ggcggcaata gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc
     2041 ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa gctccagcag
     2101 atcccggtcc attgcggcga gggctttttc ccgatcgccc aacaggaaga ccggcacgtt
     2161 ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg
     2221 ttccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa
     2281 accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta aagcatcagc
     2341 cagacgctca acggcgttac cgacccaggt gtcttgatgg agaaactcgc tttcaacaat
     2401 taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg caagtatctt
     2461 ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata tttgttttta
     2521 taaacaatat gttacgtgat tattttaaat tattttcact gcttattatc gacggctaaa
     2581 ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc ggcgctgttg
     2641 gctaagtttg cggtattgtt gcggtgacat gccgacatat ttgccgaacg tgctgtaaaa
     2701 acggctactc gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt tatcggtatc
     2761 gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa
     2821 ttgcatgacc cgctggaata tccccattgc atagttggcg ttaagtttga cgtgctcagc
     2881 cacatcgttg atggtcagcg cctgatcata gttttcggca ataaagccca gcatctggct
     2941 gacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac
     3001 cagaatcggt tcccagccag agaggctaaa tcgcttgagc atcaggccaa tttcatcaat
     3061 ggcgagctgg cgaatttgct cgttcggact gtttaattcc tgctgccagc ggcgcacttc
     3121 aaacgggcta agttgctgtg tcgccagtga tttgatcacc atgccgtgag tgacgtggtt
     3181 aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat taaaaatcgc
     3241 catgctctga caggttccgg tatctgttag ttggtgcggt gtacaggccc agaacagcgt
     3301 gatatgaccc tgattgatat tcactttttc attgttgatc aggtattcca catcgccatc
     3361 gaaaggcaca ttcacttcga cctgaccatg ccagtggctg gtgggcatga tatgcggtgc
     3421 gcgaaactca atttccatcc gctggtattc tgaatacagc gacagtgggc tacgggtctg
     3481 cttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatgactctc tttcctggaa
     3541 tatcagaatt atggcaggag tgagggagga tgactgcgaa caggcgcacg gttttattcc
     3601 tctccctgga ggtgcgagga gagcggatga gaagatttgg gtcgattatt cagcttttca
     3661 ggtaatttat tcccataaaa tcagatctat tgatgtgcca cttaggatct gagtttttgg
     3721 gaatactcaa ccgggaaacc ggaggttttc tgcgatatcg cctgccatga tgaagtcatt
     3781 caagcaagcc aggagatctg catgatgtct gcacctaaaa ttacatttat cggcgctggt
     3841 tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgtgaggc gctgaaaacg
     3901 gcgcatattg ccctgatgga tatcgatccc acccgcctgg aagagtcgca cattgtggtg
     3961 cgtaagctga tggattcagc aggggccagc ggcaaaatca cctgccacac ccaacagaaa
     4021 gaagccttac aggatgctga ttttgtggtg gtggcatttc agattggcgg ttatgaacct
     4081 tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac cattgccgat
     4141 acgctggggc cgggcggcat tatgcgtgcg ctacgtacca ttccgcacct gtggcagatt
     4201 tgcgaggaca tgacggaagt ctgccccgat gccaccatgc tcaactacgt taatccaatg
     4261 gcgatgaata cctgggcgat gtatgcccgc tatccgcata tcaagcaggt agggctgtgc
     4321 cattcggtgc agggaacggc ggaagagctg gcgcgtgacc tcaatatcga cccggctacg
     4381 ctgcgttacc gttgcgcagg tatcaaccat atggcgtttt acctggaact ggagcgcaaa
     4441 accgccgacg gcagttacgt gaatctctac ccggaactgc tggcggctta tgaagcaggg
     4501 caggcaccga agccaaatat tcatggtaat actcgctgcc agaatattgt gcgttacgaa
     4561 atgttcaaaa agctgggcta cttcgtcaca gaatcgtcag aacattttgc tgagtacaca
     4621 ccgtggttta ttaagccagg tcgtgaggat ttgattgagc gttataaagt accgctggat
     4681 gagtacccga aacgctgcgt cgagcagctg gcgaactggc ataaagagct ggaggagtat
     4741 aaaaacgcct cccggattga tattaaaccg tcacgggaat atgccagcac aatcatgaac
     4801 gctatctgga ctggcgagcc gagtgtgatt tatggcaatg tccgtaacga tggcttgatt
     4861 gataacctgc cacaaggatg ttgcgtggaa gtggcctgtc tggttgatgc taatggcatt
     4921 cagccgacca aagccggtac gctaccttcg catctggccg ccctgatgca aaccaacatc
     4981 aacgtacaga cgctgctgac agaagctatt cttacggaaa atcgcgaccg tgtttaccac
     5041 gccgcgatga tggacccgca tactgccgcc gtgctgggca ttgacgaaat atatgctctt
     5101 gttgacgacc tgattgccgc ccacggcgac tggctgccag gctggttgca ccgttaaggg
     5161 gtaatcgctt ccggcgctct ggtgattaag ccaaagcgcc gtagctggcg gcaatgaaag
     5221 cgggcgagga atatcaccgt cgttaatgcg cccagtagtg cgcagaacat atcggattgc
     5281 gtatcccacg ggtcgccttg agtaccgaga aaatcatctg cgccctggcc cattgccagc
     5341 gctgcccacc actcgattaa ctcatacatc gcgcttatcg ccagcgcgac acaacagacc
     5401 agaaacgcca ccattttacg cccgcgaacg tacatcccgc gcacgaggat ctctcgtgcc
     5461 accagtgcag gcaccagccc ctggaaaaaa tgccccagtt tatcatacgg attacggctc
     5521 aaccccagcc attcctgcac ctcaaaacca acggggactt tcgcgtaggt gtattgcccg
     5581 ccgaccatca ggatgatggc gtgaaagaaa atgagcgtat agagcagcgg cgttaacggg
     5641 taacgtttag cggtggcaag cagtagcggc acgacaataa tcaccggtgt cacttccatc
     5701 agccaggtga gtttgtcatg ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc
     5761 agtgcgccgg tgttaagaat taacggcttg agtgtgcggg tcatggtgag ttcgcaagtc
     5821 agggaaaata ctgactattg accagtaagc gtgaaattac aatcgcaaaa gaggtggccc
     5881 agggggatca cctggcagca tgctgccagg cgctgggccg aagaggttac ttagtgcagt
     5941 tcgcgcactg tttgttgacg atctgctgga agaagtcgtt acctttgtca tcgaccagga
     6001 taaacgccgg gaaatcttct acttcgattt tccagatagc ttccataccc agctccggat
     6061 aagcgacgca ttccagatgc ttgatgctct gctgcgccag taccgccgcc ggaccgccga
     6121 tgctaccgag gtagaagccg ccgtgtttat ggcacgcgtc ggtaacctgc tggctgcggt
     6181 tacctttcgc cagcatgatc atgctgccgc cgtgggattg cagcagatcc acgtaggagt
     6241 ccatacgacc tgcggtggtt gggccaagtg aacctgatgg ataaccggca ggcgttttcg
     6301 ccggacccgc gtagtagatc gggtgatctt tgatgtactg cggcagttct ttaccggcgt
     6361 caatcagctc tttcagcttg gcgtgtgcaa tatcgcgacc cacgataatg gtgccggtga
     6421 gcgacaagcg ggtggatacc gggtattgcg aaagctgggc aaggatctct ttcatcgggc
     6481 ggttaaggtc gactttcact gcttcgcctt cacctgcctg acgcagttct tctggaatgt
     6541 actggcctgg attgtgttcc agtttttcga tccagatacc ttcgcggttg attttcgctt
     6601 taatgttacg gtcagcggag caggagacgc ccatgcctac cgggcaggat gcgccgtgac
     6661 gtggcagacg gataacgcgg atgtcgtgcg cgaagtattt accgccaaac tgcgcgccaa
     6721 gaccgagttt ctgggcctct tccagcagtt cctgttccag ctggatatcg cggaacgcct
     6781 gaccatgttc gttcccttcc gtcggcagtt catcgtaata gtgagcgctt gctaacttga
     6841 cggttttcag gttggtttcc gcagacgtac cgccaatcac aaacgcgata tggtacggcg
     6901 ggcaggctgc agtaccgagg gtacgcattt tctcgacgag gaagtttttc agtttgccgg
     6961 gcgtcagcag ggctttggtt tcctggtaga gatacgtttt gttggcagag ccgccgcctt
     7021 tagcgacaca gaggaatttg tactcatcgc catctaccgc gtacaggtcg atttgcgcag
     7081 gcaggttcgt gccggtgttg acctctttat acatgtccag cggcgcgttc tgtgaatagc
     7141 gcaggttatc ttcgatatag gtgttataga cgcctttcga cagcgcttct tcatcaccgc
     7201 caccggtcca cacgcgctga ccttttttac cgacgatgat cgcggtgccg gtatcctggc
     7261 aggtcggcag cacgccttta gcggcgattt cggagtttct taagaattgc agcgccacgt
     7321 acttgtcgtt ttcgctggct tctggatcgt gaagaatagc cgcaacctgt ttctggtgtg
     7381 ccgggcggag catgaaagaa gcgtcgtgaa aggcttgctg cgccagcagg gtcagggctt
     7441 ctggttccac tttcaggatg gtttcgccgt cgaagtcggc aacgctaacg taatcagaag
     7501 tgagtagata gtattcggta ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg
     7561 gtttgtttga catagcttcc agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc
     7621 gcgtagtcac gggcggcacg caaagtgcat ttataagaac ccgtacatcg cggcgaagat
     7681 ccagccgaag acgcacgata cgctcacacc aatcaacccc ggcagaataa agctgtggtt
     7741 gatgacgaag cgaccgatgt gggtggtgcc ggaacggtca aactgaatcg ctgccagatc
     7801 gctcggataa gtcggcagga tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta
     7861 tgccggatca acgccgatcg ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg
     7921 agagtttaca aacttggaaa ccagcagcag aacaatggca taggcccacg gatactcttt
     7981 caccatttca cccagtacgc cctgaatttc agacatatgc gcaccgaaca tggtttcagc
     8041 catccatgcg ataccgtaca ccgccacgat ggcgatcata ccggaacgga agacttcgtt
     8101 ttttgagata gacgcgggat tggttttggt caggataata atcagcgccc cggtcagcag
     8161 cataaacatc tgaataacca gtaccatcga cagcggtttg ccgccgaagg atggacgcag
     8221 gtccgaatca gcaccaagaa gggcgactac agcgattgcc ccgaggaaga tccacattgc
     8281 cagccagttg cttttcggca gttttttatc cagcagcgtc gcggtatcac cgtaaacata
     8341 ctcacggttt tccggtacgg agatgaattt ttggaactct tcgtctttat ccagatcttt
     8401 accgcggaac cagctgaaga taccgatcgc caggataccg attaacgtcg atggaatggt
     8461 gattgccagc aggtcgagga actcaagatg gcgaccatca aaggtgacat tacccagcat
     8521 cgcaaccaaa gatacgaccg caaccgacac cggactggcg ataatcccca tctgtgcacc
     8581 gatagaactt gccgccatcg gacgttccgg acggatgttg ttcttaatag caacgtcgta
     8641 gatgatcggc agaatggtgt aaaccacatg acccgtaccg caaagaatgg tcagcgtaca
     8701 ggtcacaaac ggcgcgacaa ttgagacata tttcgggttg cgacgcagca gcttctcggc
     8761 aatttgcagc atgacatcaa gaccgcccga agcttgcaag gtcgccgatg ccgccaccac
     8821 cgcaatgata accagcatga catcaaccgg tggtttacct ggctgaaggt ggaagacgaa
     8881 gaccagaatg accagaccga taccgcctaa taaacccagc gcgatacccc cctttctggc
     8941 accataaaac agacatat
//
LOCUS       DAZFRG010000197         8849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFRG010000197 DAZFRG010000000
VERSION     DAZFRG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.198"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..788
                     /gene="yeeO"
                     /locus_tag="ACHKUC_003764"
     CDS             <1..788
                     /gene="yeeO"
                     /locus_tag="ACHKUC_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6619943.1"
                     /translation="YIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIP
                     ASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRR
                     LGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWL
                     NALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVW
                     MGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            889..964
                     /locus_tag="ACHKUC_003765"
     tRNA            889..964
                     /locus_tag="ACHKUC_003765"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:922..924,aa:Asn,seq:gtt)
     gene            1126..2388
                     /locus_tag="ACHKUC_003766"
     CDS             1126..2388
                     /locus_tag="ACHKUC_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6619944.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISQSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLRASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPAPPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(2582..3886)
                     /gene="ybtS"
                     /locus_tag="ACHKUC_003767"
     CDS             complement(2582..3886)
                     /gene="ybtS"
                     /locus_tag="ACHKUC_003767"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO6619945.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFVEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(3914..5194)
                     /gene="ybtX"
                     /locus_tag="ACHKUC_003768"
     CDS             complement(3914..5194)
                     /gene="ybtX"
                     /locus_tag="ACHKUC_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO6619946.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5187..6989)
                     /gene="ybtQ"
                     /locus_tag="ACHKUC_003769"
     CDS             complement(5187..6989)
                     /gene="ybtQ"
                     /locus_tag="ACHKUC_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO6619947.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(6976..8688)
                     /gene="ybtP"
                     /locus_tag="ACHKUC_003770"
     CDS             complement(6976..8688)
                     /gene="ybtP"
                     /locus_tag="ACHKUC_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO6619948.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
BASE COUNT         2108 a         2585 c         2297 g         1859 t
ORIGIN      
        1 gttatattgg cgcagttgca attttgtggg tgctggcgat tggttttaat cctgcgctaa
       61 ggatttcgtt aaagagctat tttaaaccgc tgaattttag cattatctgg gaagtcatgg
      121 ggattggtat tccagcgagt gtcgaatcag tgttatttac cagtggtcgg ttattaaccc
      181 aaatgttcgt tgccgggatg gggaccagcg ttattgccgg aaattttatc gcgttctcaa
      241 ttgcggctct tatcaactta cccggaagtg cgctcggttc tgcttctacg atcattacag
      301 gccgaaggtt gggggtaggg cagatagcgc aagcagagat tcagttgcgg catgtgttct
      361 ggctgtccac tcttggatta acggccatcg cctggctaac ggctcccttt gccggggtta
      421 tggcatcgtt ttacacccag gatccacagg ttaaacacgt cgttgtgatt ctgatttggc
      481 taaatgcttt atttatgcct atttggtccg cctcatgggt gctacccgct ggatttaaag
      541 gtgctcgtga tgcccgttac gccatgtggg tttcgatgtt gagcatgtgg ggttgtcggg
      601 ttgtagtcgg ttatgtgctg ggaatcatgc ttggctgggg tgtggttggc gtctggatgg
      661 gaatgtttgc cgactgggct gtgcgggccg tgctgtttta ctggcgaatg gttactggac
      721 gttggctatg gaaataccct cgatccgagc cgcaaaagtg tgaaaaaaag ccagttgtat
      781 cggaataaac gacaaaatgc agattatttc agcaaacgat ttcaaattta aaaaacaggc
      841 tttgacattg tgggtgggca tcgctaatat tcgcctcgtt ctcacgattc ctctgtagtt
      901 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
      961 gccaattttc tgttttcttg catccttgcg aatctttatc tgatgttgat tcaacaggtt
     1021 agtgtgaaaa ctcttctcgg tgcattttga ttttacccta cgcatcggga aaaattggtg
     1081 gtcaaatctg gggtcaggtt agttcgataa tggagtgacc cccatatatc ccttaccgac
     1141 gcaaaaatcc gcaccctcaa gccttctgat aaacccttta aagtctccga ttctcacggt
     1201 ctgtatctgc tggtcaagcc gggtggctcc cgccactggt atctcaaata ccgtattagc
     1261 ggtaaagaat cccgcattgc gctgggtgcc tatccagcca tctcccagtc tgatgcgcga
     1321 cagcaacgtg aaggtatccg taaaatgctg gcgctgaata tcaacccggt acagcagcgg
     1381 gctgctgtac gtggctcacg aacaccggag aaagttttta aaaacgtggc gctggcgtgg
     1441 cataaaagta acaggaaatg gtcgcagaac accgccgacc gtctgcgtgc cagtctgaac
     1501 aatcacatct ttccggtcat cgggaactta cctgtatcag aacttaaacc ccgtcatttc
     1561 attgacctgc tgaaagggat cgaggaaaaa ggtctgctgg aggttgcgtc ccgcacacgg
     1621 cagcacctga gtaacataat gcgccatgcg gtccatcagg agttaatcga tacgaaccct
     1681 gcagcaaacc ttggcggcgt gaccacacct cctgtcagac ggcactatcc tgccccgccg
     1741 ctggagcggc tgcctgaact gcttgaacgt attggggcat atcatcaggg ccgtgaactg
     1801 acccggcatg ccgttctgct gatgctgcat gtgttcattc gctccagtga actgcgtttc
     1861 gcccgctggt cagagattga tttcacaaac cgagtctgga cgatacccgc gacgcgagaa
     1921 cccattattg gcgtgcgtta ttccggccgc ggggcaaaaa tgcgaatgcc gcatatcgtc
     1981 cccctctcag aacagtccat cgccattctg aaacagatta aggatatcac cggtaataat
     2041 gaactgatct tccccggcga ccataacccg tataagccaa tgtgtgaaaa cacggtcaat
     2101 aaggcactgc gggtgatggg ttacgacacg aaaaaggata tctgcggtca cggcttccgg
     2161 gcaatggcat gcagtgcgct gatggaatcg ggtttatggg caaaggacgc agtagaacgc
     2221 cagatgagtc atcaggagcg caataccgtg cgcatggctt atattcataa ggcagagcac
     2281 ctggaagccc gcaaagcgat gatgcagtgg tggtcggatt atctggaagc atgccgagaa
     2341 tcttatgcac cgccttatac aattggtaaa aataagttta tcccatagta tactaacgaa
     2401 tcccacactc tggcaaatta ccaccacctc cacaggaggt ggttctatat agacttttat
     2461 caacttcatg acgaaaaaca cctattttat cccaaaagaa gacaatagag tcacagagga
     2521 atcaaagagc gactcaattg tctttggaca atctggttgt gaggttagaa aacagttact
     2581 cctacaccat taaatagggc gcaatgctcg ctaatttctc ccgggtttct gtcagttcgc
     2641 gttccggcgt agattgcgcg atgattcccg cccccgcctg tatccagcag cgctggctat
     2701 cctgaaatac ggaacgcaga actagcgccg catcaaagcg cgtatcgtcc agcagcagga
     2761 ttgcgccgga ataaagctct cgcggcgtct tctcaatttg cataatcgcg ttcagagcag
     2821 catttttagg gatacctgag gcggtaatcg acggaaacag cacggtaaac gcatcccagg
     2881 catccttgtt ttccgcaagc tgaccgctca cgccagaccc cagatgctga acgctgccgc
     2941 gctggcgaac cgacattaaa tcttcaacca ccacgctgcc cggctggcaa acggcctcca
     3001 gttcagcaat agcttctttg acagaaagga tatgctcaag cacctcttta ctgtcgtgca
     3061 gcagttctgc ctctttcgcc ttattatgct ccgggtttcc catgcgatcg cgggtgcccg
     3121 caagcggttc agtgaccact ttattgcccg tcactgacat caccagttcc gggctaaagc
     3181 ccagcgcttc gcgtccttcc tgacggaaca taaacgagcg cacaggcgtg tttgcctgcc
     3241 gcccgtataa cagcgtggcg ggcatatcaa tccgcgatgg caggggaatg gcgcgcgaga
     3301 caatcacttt gacatactcg ccacggcgga tctccgcaac ggcgcgcgca acctgttgtt
     3361 tatatgcctc accattgagg gcggtatcca ccgccagtgg tgcgttctgc gtcgttgtac
     3421 cggcctcttt tacccactcg cacaaacgtc ggcacccgtc ggcggagtcc gcataaacgg
     3481 tgacatttcc cttttcaaaa ataagttctt cacggggaac ggttaacgtc agcagcggcc
     3541 actccccggc gttaaaggca atcccccgcg catgcgccgc aaagttgaac ccaacctgtc
     3601 catatacccg acgtcctttc acctgaggat gcgccataaa acgacgcgcg cagtcggcaa
     3661 tggaatccac cgcccatttt tgctccccgg catcatcaat aaatgtcgcc tgcgttccgt
     3721 cggcattaat gtgcagccgt gcctggcacc ctttgcctaa ataccaacag ggttgacgct
     3781 catagacata acactcttct tctacgaact ggcgtaaaac gccagaaatc gtcggtagcc
     3841 attgttcctc tggtaacgcc agatgtagaa attcactgat tttcatccct tacctctctg
     3901 tgttattcgc ggatcaatca attgcgcttg tcatcagttc tcttgccgaa tgcagccgga
     3961 tataagcaac ccacgccgcc agcaacgtga atgccccggc aaacaggaag caggccgcat
     4021 agccaaaatg ttgagcaaca acgccaccga taacgcctgc cagaatggcg attgccgcat
     4081 cagtacattg aaagagggtg aagtcgacac cggcctgcaa aggcgatgaa agcgacatca
     4141 gcgtggcgta aagacagacc agcgcgcagg ccagcgaaat ggactggaca atgaccagac
     4201 actggagaat ctgcagcagc agatgacccg gcgccatcat gagcgtcatc accaccgcca
     4261 gcagcgcgat cccctggacg ccataagccg tcaacagcgc tctgccgggt gaggtgtatt
     4321 tcatcagcaa tccgccagcc agcgttcctg ctatgcccgc tgcaatattg ccgccgctga
     4381 acagcgcgcc caatgcggac atgctcaacc catgatccaa caacagcggt gccagaagag
     4441 gcagcacaaa ccgcatgcct gaattcagca tcaataccag cagtaagccc aggcgcgcct
     4501 gcttcctcct tagcgcataa cctaacgccg gaacatgcgg gatagtcgct gttcgcgtcg
     4561 gctccgtaat gcgccacagc gggagtgaca gcgccataat cagcaccgcc atcatcagca
     4621 tggcgacagg ccagccggat gccgccgaca gcatcaggaa caccccgccg ccgcacatca
     4681 ttcccagata gctgccgccg acctgcacgc tgtttcccca gccgtaaccc gtgcgggtca
     4741 gttgatcgac acaaaagccg tcgctggcga tatcaatggt ggaggcgacc gtgcctgcca
     4801 ccataaacag aacaaataac gcgacgattt gcgtatcggg gaatccccct tctcgcccaa
     4861 accagccaat cgctgcggca atagtcagga tcgccgctag cgcgacctga ccacgcagga
     4921 ttaacatgcg ggaacggcgt tcctggctac cgggcggaag ccgccagcgc tcgatccacg
     4981 gcgcccaaat aaacttcagc gcccagggca gcataaacag ggttgtcgca ccggcaagcg
     5041 ccagcgaacc gccagcagcg cgcaccagcg cgggtaagga ctgcatagac agcgccgata
     5101 ccagactctg cgtcacatag acgccggcaa tagcaaaaat cacccgcccg gtcgtcaacg
     5161 tcagcggttt cacattcgac tgaacatcac tcatgcaccc actctccaga cgcggaaacc
     5221 ccgtcgtcgc gccacacgcg cgcggccatt tgcgcctgcc acagcgcctg ataacgccca
     5281 tgatgtgaga gcaattgcgc atgagtaccc tgctcaacca cctgtccctc ttccatcacc
     5341 agaatgtttc cggccccggc gatggtggat aaacggtgag cgatgataat caccgtccgg
     5401 ttgtaaacca ggttatcgat cgctttttgc accgccagct cgctttcaat atccagcgcg
     5461 gcagtcggtt catcgagaat gacgaccggc gcgtttttca ataacgctct ggcaatggaa
     5521 atccgctggc gctcgccgcc cgatagctgg ccgcccatct ctcccattgg cgtcagccag
     5581 ccttgcggaa gacgggaaat aaactcaagg cactgcgccg cgcgggcggc ctcttctacc
     5641 tcctgccgcg tcgcctgtgg gcgcgcgata cggatattcg ccagcagcgt gtcatcaaac
     5701 agccagacat cctggaaaac aacagaaatc aggctattga gctgttccgg cgtcaggcgg
     5761 cgaatatcga cgccgccaat agaaatctgc ccttgctgcg gatcggcata gcgcattaac
     5821 agtttggtga ccgtagtttt gcctgcgccg gaggcaccca ccagcgcgct catactggct
     5881 gccgggaacg tcaaagaaac atgattaagc gcgtggccgt caccttcttc gtagcgatag
     5941 ctgacgttgt caaagcggat atcgtaacgt tccggcatct cgctttgctc tgcgacggga
     6001 agcggtgcta tcgccataaa ctgctcaatc cgttgcaggg cgctggcgat cagttccaca
     6061 accgaggtgt agctgataaa catcgccatc ggttctgcga agcgcataat catcgcgacg
     6121 gcggcaatca aaaaggcgag gttcagagtg cccgtcacta cccagacgat cccggataac
     6181 accaccacct gtaggcccaa ctccacgacg ctggcgatca gcatcgtcgc gccagcgccc
     6241 tgacggtgag tgcgggtctg taagttttcc agcgcattga aatgcgccag cagcgcccgg
     6301 cttttatcgg catcgctgcc gcaggtgcgc aataccatca tcccctgagc aaattcaacg
     6361 atatcgccgc tcagacgctg gtgcgcttcc cccagcgtct gcatttgtcg ccgcatcgcc
     6421 gggcggcgcc agtaataaaa cggcaccagc agagggaaga tcagcaacat caccagcccc
     6481 agccgccagt ctatccacaa tgtcgccagc gacgccgtca gcggcgtgac aatggtgagc
     6541 agcaaaatat tggctatcgc aataacataa ttgaggtttt catccacgct gcccagcagc
     6601 aaggcgttca tttcacccgc cctgccgcgc tggagcttct ccagcggcac gcggcgtaac
     6661 tgctcaccaa gtcgcaggcg caactcatgg gtagcctgcg ccagatgacc acggtattca
     6721 aagcccaggc cataccagcg tagcaccagc gtcacaattg cggcgacgct gaaggccata
     6781 gcccagttaa gaagttgcgg cgcgtctccc cgtaacagcg catcaatgat cggataaaga
     6841 caggcaaagg cgatcccctg catgaatgcc gccagcaaca gcgccagcat actgcgtcgc
     6901 agcatcctgg cctgactgcc aacgctggag ataagctggc gccagatgac gcgccaggcc
     6961 aggttatccg caggattatt gtctttcatt ttccaccacc tcttcctgtg ttccacccgg
     7021 cacccaatgc tgcgcctgct gatagtgttg ccagagccgt tgatacaggc cgccctgcgc
     7081 caacagttgc gtatggttcc ccatttccct gagctgtccg tcggaaaaca gcaatatcac
     7141 atcggcctga gtcaccattg agagacgatg cgcgaccatg atgaccgtcc ggccacgcat
     7201 ggccgccgcg agcgccttga taagcgccgc ttcgttttcc gggtcagcaa acgccgtcgc
     7261 ctcatcgagc accaggatgg ggcgatcctg caaaagcgcc cgggcgatag taatgcgctg
     7321 ccgctggccg ccggagagaa ataccccacg ttccccgact cgagtgttgt aaccttctgg
     7381 cagagcgcta ataaaatcat gggcctgcgc cactctggcc gccgctataa ccgcctccag
     7441 cggcgtatcc ggcgcgccca gacgaatgtt attggctatc gtgtcggcaa aaaggaagtt
     7501 gtcctgaaac acaaacgaga gttgcttcat cagggtgtcc gtctgcatat cacgcagatc
     7561 cacgccgcca atacggatat ggcctttgtc cgggtcggcg taacgcagca gcaagcgcgc
     7621 cacggtgctt tttccggcgc cgcttggccc gaccagcgcc acaatttgcc cggcaggcac
     7681 atgaaagctc acctcctgca acgcggcgcc agtacgcgct tgcggataat gaaagctcac
     7741 ctgctcaaag gtaatgctcg cctcttgcgg ctgctggtca gactgcggca gcgataactc
     7801 cggcatcgcg agaacctgat aaatacgctg aatgcttaaa cgcgtttgcg cgaccaggtt
     7861 attgagcatc atcattggca ttacggcttc ggccattccg ctgcccagca gtaaaacggc
     7921 cacccacgcg ataaaatcga aactgccata gtgcaacagg ccgtatcccg accagatcag
     7981 gacaaacagg gtcgggagag gattcagaat cgagaaggaa aaacgcgctg aaaaaccggc
     8041 tttgcgatac caggttttga gcacatcgac ccactcttca agggcgcgtt gatagcgcaa
     8101 aaaactggtg ctgccgctat caaacgttct caccacgggc atggcctgca cgaactcaat
     8161 aaccgccgcg ctaacctgct cgcgcgcctt atgataacgc tgagccatat cttccgaacg
     8221 ctggcgggcg agcacgagaa caaccgatcc aaacgccagt acccccagcg tcgcgattgc
     8281 cagccgccag tccagccaga acaagataac gattgtcgcc agcggcatga tgatcgcgcg
     8341 ggcatagagc ggcgtgctgt cggccacaaa aatatgtaac gacttcacgt catccagcat
     8401 cactttcgcc actgacccgc tgcccatttg ctgtaacacg ccaggcggaa gctgcaatgc
     8461 tttacgggct aacctgctgc gcagaatgtt ctccagatga aaagccgcgt aatgagaggt
     8521 gttgaacgcc tgtaagcgca gaacaaaagt taagaccacg cagccgatca cgccgcccag
     8581 cggccaggcc ggaatagcgt caggcgtcgc gcgaatgtca cgcaggctcc acgccagaaa
     8641 aaggagcgcc cccaggctgg ttaatgccgc cagccccgcc agcgccatag ctaaaataac
     8701 cttcctgcga accggtgtaa tcacctgcca gagaggaaat cgggacagcg attcagtatt
     8761 ggatgattga gatgacatgg gagtaactga atttcctgat gaatttttac ggacgccaaa
     8821 acgacaattc agggtggcgt tctgagaat
//
LOCUS       DAZFRG010000198         8818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFRG010000198 DAZFRG010000000
VERSION     DAZFRG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.199"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..1372
                     /gene="wcaK"
                     /locus_tag="ACHKUC_003771"
     CDS             92..1372
                     /gene="wcaK"
                     /locus_tag="ACHKUC_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO6619949.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            1369..2589
                     /gene="wcaL"
                     /locus_tag="ACHKUC_003772"
     CDS             1369..2589
                     /gene="wcaL"
                     /locus_tag="ACHKUC_003772"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO6619950.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISRREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLSSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDACALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2600..3994
                     /gene="wcaM"
                     /locus_tag="ACHKUC_003773"
     CDS             2600..3994
                     /gene="wcaM"
                     /locus_tag="ACHKUC_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO6619951.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQIVKINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCENINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGRMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKPASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4169..5062
                     /gene="galF"
                     /locus_tag="ACHKUC_003774"
     CDS             4169..5062
                     /gene="galF"
                     /locus_tag="ACHKUC_003774"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO6619952.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQAVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            5435..6520
                     /gene="rfbB"
                     /locus_tag="ACHKUC_003775"
     CDS             5435..6520
                     /gene="rfbB"
                     /locus_tag="ACHKUC_003775"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6619953.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EISDSERYSFEHADICDAEAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRH"
     gene            6520..7419
                     /gene="rfbD"
                     /locus_tag="ACHKUC_003776"
     CDS             6520..7419
                     /gene="rfbD"
                     /locus_tag="ACHKUC_003776"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO6619954.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTVYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            7477..8352
                     /gene="rfbA"
                     /locus_tag="ACHKUC_003777"
     CDS             7477..8352
                     /gene="rfbA"
                     /locus_tag="ACHKUC_003777"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6619955.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLHYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGHLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            8360..>8818
                     /gene="rfbC"
                     /locus_tag="ACHKUC_003778"
     CDS             8360..>8818
                     /gene="rfbC"
                     /locus_tag="ACHKUC_003778"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO6619956.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESYNQRVFEEAVGRKVE
                     FVQDNHSKSRKGVLRGLHYQLEPYAQAKLVRCIEGEVFDIAVDIRKSSPFFGKWVGVT
                     LSAENKRQLWIPEGFAHGFVVISDTAEFVYKTNNYYSQQAERSIIFDDKDL"
BASE COUNT         2394 a         1993 c         2203 g         2228 t
ORIGIN      
        1 ctgataagac gcgttagcgt cgcatcaggc aaaaaacggc aacgaacaca acaaacacag
       61 cctcacctgg catcaaattt atgaggaaaa aatgaaatta cttattctgg gcaaccacac
      121 ttgcggcaat cgtggcgata gcgccatcct gcgtggctta cttgatgcca tcaatatcct
      181 caatccacat gccgaagtgg acgtgatgag ccgctatccg gtcagttctt cctggctgct
      241 caaccgcccg gtaatgggcg atccgctgtt cctgcaaatg aaacaacaca acagcgcggc
      301 gggcgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc taccagcacc aggtattgct
      361 ctcacgcgtc accgacactg gcaagttgcg taatatcgcc atcgcccagg gattcactga
      421 cttcgtgcgc ctgctgtcag gttacgacgc cattatccag gtcggcggat cgttttttgt
      481 cgatctctac ggcgtgccgc agtttgaaca tgcgctttgc actttcatgg cgaaaaagcc
      541 gctgtttatg attggtcaca gcgtcggccc cttccaggat gagcaattta accaactggc
      601 gaactatgtc tttggtcact gcgacgcgct gatcctgcgc gaatcggtca gccttgattt
      661 gatgaaacgc agcaatatca ccactgcaaa agttgaacat ggcgtcgata ccgcgtggct
      721 agtcgatcac cacattgaag acttcaccgc cagctatgct gttcaacact ggctggatgt
      781 tgccgcacaa cagaaaacgg tggcaattac cctgcgcgaa ctggcaccgt tcgacaaacg
      841 tctcggcacc actcaacaag cgtatgaaaa agcctttgcc ggggtggtca atcgcattct
      901 cgacgaaggc tatcaggtga ttgcgctctc cacctgtacg ggcatcgaca gctataacaa
      961 agacgaccgc atggtggcgc tcaacctgcg ccagcacatc agcgatcctg cccgttacca
     1021 cgtagtgatg gatgaactca atgatctgga aatgggcaaa attctggggg cctgtgaact
     1081 caccgtcggt acgcgcctgc actctgccat tatctcgatg aactttgcca ctccggcgat
     1141 tgccatcaac tatgaacata aatccgctgg gattatgcaa caactggggc taccagagat
     1201 ggcaattgat atccgtcatt tattagacgg cagcctgcaa gcgatggttg cggatacctt
     1261 aggccagctt ccggcgctga acgcacgact taacgaagct gtcagtcgcg aacgtcagac
     1321 gggaatgcag atggtgcaat ctgtgcttga acgcatcggg gaggtgaaat gaaggtcggc
     1381 ttctttttac tgaaatttcc gctgtcgtcg gaaaccttcg tcctcaatca aattaccgcg
     1441 tttattgata tgggatttga ggtggagatt gtcgcgctgc aaaaaggcga cacacaaaac
     1501 acccacgcgg catggacgaa atacaacctt gctgccagaa cccgctggtt acaggacgaa
     1561 cctgcgggca aagtggcgaa actgcgccac cgcgccagcc agaccttacg cggcattcat
     1621 cgtaaaaata cctggcaggc gctcaacctc aaacgctatg gtgctgagtc gcggaacctg
     1681 attttgtctg ccatttgtgg tcaggtcgca acaccgtttc atgccgatgt gtttatcgct
     1741 cattttggcc ctgctggggt aaccgcggca aaactacgcg aactgggtgt cattcgcggc
     1801 aaaattgcca ctatcttcca cggtattgat atctccaggc gggaagtgct caaccactac
     1861 actcccgaat atcagcaact gttccgccgt ggcgatctga tgctaccgat aagcgatctg
     1921 tgggccggaa ggctgcaaaa aatgggctgc ccgagggaaa aaatcgccgt atcgcgcatg
     1981 ggcgtggaca tgacgcgctt tagcccgcgt ccggtgaaag cacctgcaac gccgctggaa
     2041 atcatttccg tcgcacgctt aaccgagaaa aaaggcctgc atgtggcgat tgaagcctgc
     2101 cgtcagttga aagagcaggg cgtggcattt cgctatcgca tcctcggcat tggcccgtgg
     2161 gaacgacgcc tgcgcaccct catcgaacaa tatcaactgg aagatgtgat agagatgccg
     2221 ggctttaaac cgagccatga agtgaaagcg atgctcgatg acgcggatgt cttcctgttg
     2281 tcatcggtta caggtgcgga tggcgatatg gaaggtattc cggtggcgct aatggaagcg
     2341 atggcggttg gcattccggt tgtttctact ctgcacagcg gaataccgga actggtggag
     2401 gccgataaat ccggctggct ggtgcctgag aacgatgcct gtgcactggc gcaacgactg
     2461 gcggcgttta gccaactgga caccgacgaa cttactacgg ttgtcaaacg tgcgcgcgaa
     2521 aaagtcgaac acgattttaa ccagcaggtg attaatcgag aactcgccag cttgctgcag
     2581 gctttataga ggagaacgta tgccatttaa aaaactctcc cgacgcacct tcctgacggc
     2641 aagctcggcg cttgccttcc tccatacccc tttcgctcgc gcgcttcccg cccgacaaat
     2701 cgttaagatt aacgactaca acccacacga ctggatcgcc tcatttaaac aagccttcag
     2761 cgaagggcaa acggtcgtcg tgcctgccgg attcgtttgt gaaaatatca acaccggcat
     2821 cttcattcct cctggcaaaa cgttacacat ccttggaagc ctgcgcggca acggcagagg
     2881 gcgttttgtc ttacaagacg gcagccaggt gacaggggag aagggcggcc gtatgcataa
     2941 catcaccctg gatgtgcgcg gttctgactg catcatcaaa gggttggcga tgagcggttt
     3001 tggcccggta acgcagattt atatcggcgg caaaaacaaa cgggtcatgc gcaacctgac
     3061 catcgataac ctcactgtca gccacgctaa ttacgccatc ttacgtcagg gatttcataa
     3121 ccagattatc ggtgccaata tcaccaattg taagttcagc gacttacagg gcgacgccat
     3181 tgaatggaac gtggcaatta acgacagtga tattttgatc tccgaccatg tgatcgagcg
     3241 catcaactgt actaacggca aaatcaactg gggcattggc ataggccttg cgggaagtac
     3301 ctacgataat aattatccgg aaaaccaggc agtgaaaaac tttgtcgtgg cgaatatcac
     3361 gggatcagat tgtcggcaac tgatccatgt tgaaaatggt aaacattttg ttatccgtaa
     3421 tatcaaagct cgtaatatca cgccggattt cagtaagaaa gcaggtattg ataacgccac
     3481 agttgctatt tatggttgtg acaactttgt gattgataat attgaaatga ttaatagtgc
     3541 cgggatgtta attggctatg gggtaattaa aggcaaatat ctctcgatac cgcagaattt
     3601 ccgagtgaat gatattcaac tggataatac tcatcttgct tataaattgc gcggcatcca
     3661 aatctccgcc gggaatgctg tctcctttgt ggcgctgact aacattgaga tgaagcctgc
     3721 gtcgcttgag ctacacaata aaccgcaaca cctttttatg cgaaatatca atgtgatgca
     3781 ggaatcctct gttggacccg cattgagcat gaacttcgac atgcgcaaag acgtacgcgg
     3841 tgtctttatg gcgaaaaaag aaacactgct gtcacttgca aatgttcatg cgatgaatga
     3901 aaaaggacaa agctccgtcg atatcgacag gattaatcac catattgtta atgtggaaaa
     3961 gattaacttt agattgccgg aacggagaga gtagatttgc gaccattcct ggaaaaatgg
     4021 agccatactt aggaacaatg ctactgcaat ccacaacgaa gcggcgtaac atcacaagta
     4081 attcagtaat caattcaggg taattgatgc tggcgaaaaa aatcgaacaa gctataattc
     4141 agcaaccatt ttacaggtgg atgaaataat gacgaattta aaagcagtta ttcctgtagc
     4201 gggtcttggg atgcatatgt tgcctgccac taaggcgatt cccaaagaga tgctaccgat
     4261 cgtcgacaag ccaatgattc agtacatcgt tgacgagatt gtggctgcag ggatcaaaga
     4321 aatcctcctg gtaactcacg cttccaagaa cgcggtcgaa aaccacttcg acacctctta
     4381 tgaattagaa tctctccttg aacagcgcgt gaagcgtcaa ctgcttgcgg aagtgcagtc
     4441 catctgtcca ccgggcgtga ccattatgaa cgtgcgtcag ggcgaacctt taggtttggg
     4501 ccactccatt ttatgtgcac gacctgctat tggtgacaac ccatttgtcg tggtactgcc
     4561 agacgttgtt atcgatgacg ccagcgccga cccgctgcgt tacaaccttg ctgccatgat
     4621 tgctcgtttc aacgaaacgg gccgcagcca ggtgctggca aaacgtatgc cgggtgacct
     4681 ctctgaatac tccgttattc agaccaaaga accactggat cgtgaaggta aagtcagccg
     4741 cattgttgaa tttatcgaaa aaccggatca gccgcagacg ctggactcag acatcatggc
     4801 cgtgggccgt tatgtgcttt ctgccgatat ttggccggaa ctggaacgta ctcagcctgg
     4861 tgcatgggga cgtattcagc tgactgatgc cattgctgag ttggcaaaaa aacaagcagt
     4921 tgacgcaatg ctgatgactg gggacagtta cgactgcgga aagaaaatgg gttatatgca
     4981 agcgtttgtg aagtatgggc tgcgtaacct gaaggagggc gctaagttcc gcaaaggcat
     5041 tgagaagttg ttaagcgagt aacaaattcc tgactggaag tgtcggtcag taagtaatta
     5101 aaaacggcag tgaagattca tagcataagt tttgctcatt gaatctcact gccgttgtct
     5161 tttttataag tacaataaaa acagcatgtt agcttgcaat catagttcaa atttttccag
     5221 gattttcctt gtttctacag ctgtttggta agacaattag cgtttgaatt tttcggattt
     5281 ggcacgagtg ggttacgctc gtcacatcgt ggaaatgtac gcagtgctct ggtagctgtt
     5341 aagccagggg cggtagcgtg cattaatgca tctattaatc aaatctggag cagtctattt
     5401 cacagcatgc tctctggcta tatggaataa aaaagtgaag atacttgtta ctggtggcgc
     5461 aggatttatt ggttctgctg ttgttcgtca cataataaat aatacgcaag atagtgttgt
     5521 taatgtcgat aaattaacat acgccggaaa cctggaatcg ctcgctgaaa tttctgattc
     5581 tgaacgttat tcatttgagc atgcagatat ctgcgatgcc gaagcgatgg ctcgtatttt
     5641 cgcacagcac cagccagacg cggtgatgca cctggcagca gagagccacg ttgaccgctc
     5701 aataactggc cctgcggcat ttattgaaac caatattgtg ggtacttatg ttcttttaga
     5761 agcggcgcgc aattattggt ctggtctgga tgatgaaaag aaaaaaaact tccgctttca
     5821 tcatatttct actgatgagg tgtatggtga cttaccccat ccggatgaag taaatagcaa
     5881 tgaaacgttg ccgctattta cggaaatgac agcatacgcg ccaagtagtc catattctgc
     5941 ttctaaagct tccagcgatc atttggttcg cgcatggaaa cgtacttatg gtttaccgac
     6001 cattgtgact aattgctcga acaactatgg tccttatcat ttcccggaaa agcttattcc
     6061 actggttatt cttaatgcac tggaaggtaa ggcattacct atttatggca aaggagatca
     6121 gatccgcgac tggttgtatg tagaggatca tgctcgagcg ttatataccg tcgtaaccga
     6181 aggtaaagcg ggcgaaactt ataacattgg tggacacaac gaaaagaaaa acatcgacgt
     6241 agtgttcact atttgtgatt tgttggatga gatagtcccg aaagagaaat cttatcgtga
     6301 gcaaattacc tatgttgctg atcgcccagg gcatgatcgc cgttatgcaa ttgatgccga
     6361 taaaattagc cgcgaattgg gctggaaacc acaggaaacg tttgagagcg ggattcgtaa
     6421 aactgtggaa tggtatctgt ccaatacaaa atgggttgat aatgtgaaaa gtggtgccta
     6481 tcaatcgtgg attgaacaga actatgaggg ccgccactaa tgaatatcct cctttttggc
     6541 aaaacagggc aggttggttg ggaactacag cgtgctctgg cacctctggg taatttgatt
     6601 gctcttgatg ttcactccac tgattactgt ggtgatttta gtaaccctga aggtgtggct
     6661 gaaaccgtta gaagcattcg gcctgatatt attgtcaacg cagccgctca caccgcagta
     6721 gacaaagcag aatcagaacc ggagtttgca caattactga acgcgacgag tgtcgaagcg
     6781 atcgcgaaag cagccaatga agtcggcgct tgggttattc actactctac tgactacgta
     6841 tttccgggga ccggtgaaat accatggcag gaggaggatg caaccgcacc gctaaatgtt
     6901 tacggtgaaa ccaagttagc aggagaaaaa gcattacaag agcattgtgc gaagcacctt
     6961 attttccgga ccagctgggt ctatgcaggt aaaggaaata acttcgccaa aacgatgttg
     7021 cgtctggcaa aagagcgtga agaattagcc gttattaatg atcagtttgg tgcgccaact
     7081 ggcgcagagt tgctggctga ttgtacggca catgccattc gtgtggcact gaataaaccg
     7141 gaagtcgcag gtttgtacca tctggtagcc agtggtacca caacctggca cgattatgct
     7201 gcgctggttt ttgaagaggc gcgcaaagca ggcattcccc ttgcactcaa caagctcaac
     7261 gcagtaccaa caacagtcta tcctacacca gctcgtcgtc cacataactc tcgccttaat
     7321 acagaaaaat ttcagcagaa ctttgcgctt gtcttgcctg actggcaggt tggtgtgaaa
     7381 cgcatgctca acgaattatt tacgactaca gcaatttaat agtttttgca tcttgttcgt
     7441 gatggtggaa caagatgaat taaaaggaat gatggaatga aaacgcgtaa aggtattatt
     7501 ttagcgggtg gttctggtac acgtctttat cctgtgacta tggctgtcag taaacagctg
     7561 ttaccgattt atgataaacc gatgatctat tacccgctct ctacactgat gttggcgggt
     7621 attcgcgata ttttgattat cagcacgcca caggatactc ctcgttttca acaactgctg
     7681 ggtgatggga gccagtgggg gctaaatctt cactacaaag tgcaaccgag tccggatggt
     7741 cttgcgcagg catttatcat cggtgaagag tttatcggtg gtgatgattg tgctttggta
     7801 cttggtgata atatcttcta cggtcacgac ctgcctaagt taatggatgc cgctgttaac
     7861 aaagaaagtg gtgcaacggt atttgcctat cacgttaatg atcctgaacg ctatggtgtc
     7921 gttgagtttg ataaaaacgg tacggcaatt agcctcgaag aaaaaccgct acaaccaaaa
     7981 agtaattatg cagtaaccgg gctttatttc tatgataact acgttgtgga aatggcgaaa
     8041 aatcttaagc cttctgcccg cggtgaactg gaaattaccg atattaaccg tatttatatg
     8101 gaacaggggc atttatctgt tgccatgatg ggacgtggtt atgcatggct ggacacgggg
     8161 acacatcaga gtcttattga agcaagcaac ttcattgcca ccattgaaga gcgccaggga
     8221 ctaaaggttt cctgcccaga agaaattgct tatcgtaaag gatttattga cgcagagcag
     8281 gttaaggtat tagccgagcc gctgaagaaa aacgcttatg gtcagtattt gctgaaaatg
     8341 attaaaggtt agtaataaaa tgaatgttat taaaacagaa attccagacg tactgatttt
     8401 tgaaccgaaa gtttttggtg acgagcgcgg tttctttttc gaaagctata accagagggt
     8461 ttttgaggaa gctgtaggtc gcaaagttga gtttgttcag gataaccatt ctaaatcgag
     8521 aaaaggagta ttgcggggat tgcattatca attagagccg tatgcgcaag caaaacttgt
     8581 gcgttgcatt gagggtgaag tatttgatat tgctgtagat atacggaagt catctccatt
     8641 ttttggtaaa tgggttggtg taacattatc cgctgaaaat aaacgtcaat tatggatccc
     8701 tgaagggttt gctcatggtt ttgtggtgat tagtgatact gcggaatttg tctataaaac
     8761 gaacaattat tacagtcaac aagcagagcg aagcataatt tttgatgata aagactta
//
LOCUS       DAZFRG010000199         8805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFRG010000199 DAZFRG010000000
VERSION     DAZFRG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.200"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            354..920
                     /gene="mepS"
                     /locus_tag="ACHKUC_003779"
     CDS             354..920
                     /gene="mepS"
                     /locus_tag="ACHKUC_003779"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO6619957.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1101..2657
                     /locus_tag="ACHKUC_003780"
     CDS             1101..2657
                     /locus_tag="ACHKUC_003780"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6619958.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2739..4553
                     /locus_tag="ACHKUC_003781"
     CDS             2739..4553
                     /locus_tag="ACHKUC_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO6619959.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            4554..5648
                     /locus_tag="ACHKUC_003782"
     CDS             4554..5648
                     /locus_tag="ACHKUC_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO6619960.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5648..6673
                     /locus_tag="ACHKUC_003783"
     CDS             5648..6673
                     /locus_tag="ACHKUC_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6619961.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFSWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6675..8264
                     /gene="yejF"
                     /locus_tag="ACHKUC_003784"
     CDS             6675..8264
                     /gene="yejF"
                     /locus_tag="ACHKUC_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO6619962.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8268..8612)
                     /locus_tag="ACHKUC_003785"
     CDS             complement(8268..8612)
                     /locus_tag="ACHKUC_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO6619963.1"
                     /translation="MTPLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         2089 a         2204 c         2185 g         2327 t
ORIGIN      
        1 gcctcctgct ttcatccatc gagcgcaaaa atcattctct aagtaaatga atggattgca
       61 tgcgtttcac tcaatcgtac tttaattgac caatcccgct gattaacttt ctgtatcact
      121 ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg
      181 aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca
      241 cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg
      301 cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca
      361 aatctcaacc gattttgaga tatatcttgc gcgggatccc cgcgattgca gtagcggttc
      421 tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag
      481 tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta
      541 atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgctatc
      601 gtctgggcgg cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc
      661 gtgagcaatt tggcttagaa cttccgcgtt cgacttatga acagcaggaa atgggtaaat
      721 ctgtttcccg cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg
      781 gacgccatgt cggtatttat atcggcaaca accagtttgt ccatgcttcc accagcagtg
      841 gtgttattat ttccagcatg aatgaaccat actggaagaa gcgttacaac gaagcacgcc
      901 gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt
      961 taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt
     1021 ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat
     1081 ttcaggaaat taacggaatc atgtttatac gcgctcccaa ttctggacgt aagctcctgc
     1141 ttacctgcat tgttgcaggc gtgatgattg cgatactggt gagctgcctt cagtttttag
     1201 tggcctggca taagcacgaa gtcaaatacg acacactgat taccgacgta caaaagtatc
     1261 tcgataccta ttttgccgac cttaaatcca ctactgaccg gctccagccg ctgaccttag
     1321 atacctgcca gcaggctaac cccgaactga ccgcccgcgc ggcgtttagc atgaatgttc
     1381 gtacgtttgt gctggtaaaa gataaaaaaa cattctgttc atctgcaact ggtgagatgg
     1441 acattccact caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcaa
     1501 tcttacccgg cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc
     1561 ctttgctgaa aaatagcggc gtgtttgccg ctctgaatct caacctgacg ccttcactct
     1621 tttatagttc acggcaggaa gattacgatg gcctagccct cattattggt aatactgcgc
     1681 tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg
     1741 aaactaaaat tgcgggcatt cctctgactg ttcggcttta tgcagatgac tggacatgga
     1801 acgatgtgtg gtacgcattt ttactgggtg gcatgagtgg aactttcgtt ggacttctct
     1861 gctattacct gatgagtgtg cgtatgcgcc caggcagaga aatcatgacc gccatcaagc
     1921 gcgaacaatt ttacgtggtg tatcaaccgg tggttgatac acaagctttg cgggtaacgg
     1981 gcctggaagt actgctacgc tggcggcatc cagtagcagg tgaaatcccc ccggatgcct
     2041 tcattaactt tgccgaagcg caaaagatga ttgtgccact gactcagcac ctgtttgagt
     2101 tgattgcccg cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca
     2161 ttaacattgc gccggcccac ttgcacagcg aaagctttaa agcggatatc cagaaactgc
     2221 tcacttccct gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc
     2281 tgaaagagcg agaagccaca caactcttcg cctggctgca ttcggtcggc gtagaaattg
     2341 ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg
     2401 attatctgaa aattgatcgt ggatttatca acgccatcgg tacggaaacg atcacttcac
     2461 ccgtacttga cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttgctgaag
     2521 gggtcgaaac gccagaacag gcacgatggc taagcgaacg cggcgttaat ttcatgcaag
     2581 gctactggat tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt
     2641 atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg
     2701 attgcggcta atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc
     2761 tgtttatcgc tctgttcacc tttggtgcgc aggcgcaggc tatcaaggaa agctatgcct
     2821 ttgccgtact gggcgaaccc cggtacgcat ttaatttcaa ccattttgat tatgtgaacc
     2881 ccgccgcgcc aaaaggtggg caaataacgt tgtccgccct cggcaccttc gataatttca
     2941 accgctatgc actacgcggc aacccgggcg cacgcaccga gcagctgtac gacacgctat
     3001 ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa agcgcacgct
     3061 atgctgacga ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg
     3121 gttcgcccat tactgcccgc gatgtagagt ttacttttca aaaatttatg accgaaggcg
     3181 tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg
     3241 tgcgcattga gttagctaaa cccggcaaag aagatatgct gagtctgttt tcgctgccgg
     3301 tatttccaga aaagtactgg aaagatcaca aacttagcga cccgctcgcc acgcctccgc
     3361 ttgccagtgg tccgtaccgc attacgtcct ggaaaatggg gcaaaatatt gtctattccc
     3421 gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca
     3481 ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg
     3541 cctttgattt gcgtatggaa aacgacgcca aaaactgggc cacgcgctat accggtaaaa
     3601 atttcgataa aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc
     3661 gctggctggc gtttaatatc caacgtccgg tattcagcga tcgccgggtg cgggaagcga
     3721 tcactctcgc ctttgacttt gaatggatga acaaagcgtt gttttacaat gcctggagtc
     3781 gcacgaacag ttattttcag aataccgaat atgcggccag aaattacccc gacgccgcgg
     3841 aactggtgct tctggcacca atgaaaaaag atctaccgcc agaagtcttc acgcaaatct
     3901 accagccgcc ggtatccaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca
     3961 aacttctcaa cgaagcaggc tgggtgctga agggtcagca acgcgttaat gtcacaacgg
     4021 gtcagccact cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc
     4081 cgttccagca cagcctgcaa cggctgggta tcaacatgga cattcgtaag gtggataact
     4141 cgcaaatcac caaccgcatg cgcagtcgcg actatgacat gatgccgcgc gtatggcggg
     4201 cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca
     4261 cttataatgc ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg
     4321 ccgcgcaggg aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa
     4381 cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg
     4441 ataaattctc ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt
     4501 atgacgttaa taaagcgacg aaactgccgt cagccagaca acagggagag tagatgggcg
     4561 cttatctgat tcgccgtctg ttgctggtga tcccaacgtt atgggcgatt atcactatca
     4621 actttttcat cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc gccgccattg
     4681 agtttggtaa tgccggagta ttacccggcg caggcggtga aggtgttcgc gccagccatg
     4741 cgcaaacggg cgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc
     4801 cagaagtgat cgccgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt
     4861 acttcaaaat gctctgggac tacatccgtt ttgattttgg cgacagcctt tttcgcagcg
     4921 cctcggtgct gacgctgatt aaagacagtc tgccggtctc catcaccctc ggattgtgga
     4981 gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg
     5041 ggagccgttt tgacgtctgg agtagtgcat ttatcatcat cggctacgcc attccggcct
     5101 ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc
     5161 ctctgcgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg
     5221 attatctgtg gcatatcacg ctgccggtgc tggcgacagt gattggcggc tttgcggctc
     5281 tgaccatgct gacaaaaaac tcattccttg atgaagtgcg taagcaatac gtggtgacgg
     5341 cacgtgcgaa aggggtaagt gaaaaaaata ttctctggaa acatgtgttc cgcaacgcca
     5401 tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc
     5461 tgctgattga ggtgatgttt tcactcaatg gcctcggctt actgggctac gaagcgaccg
     5521 tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc
     5581 tgctgaatat tgtcagtgat atcagctata cgctggtcga tccgcgtata gattttgagg
     5641 gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgccataa
     5701 ccgtcgcggc tactggtcgt tatggatttt cctcgtcttg tttggtttga gtttgtgttc
     5761 tgaacttatc gccaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc
     5821 gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta
     5881 tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt gggctccgat
     5941 tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc
     6001 ccggcaaaac tggctgggaa cggatgccaa tggcggcgat gtactggcac gcattctcta
     6061 tggcacgcgg atctcagttc tgtttggcct gatgctgacc ctctgctcca gcgtgatggg
     6121 cgtgctggcg ggggcgctac aaggctatta cggtggtaaa gtcgatctct gggggcaacg
     6181 ttttattgaa gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt
     6241 cgtacagcct aacttctggt ggctgctggc aattactgtc ttgtttagct ggatgagtct
     6301 ggtcggcgtg gtgcgggcgg agtttttacg tacccgtaat ttcgactaca tccgcgcggc
     6361 gcaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc caaatgccat
     6421 ggtcgctacc ctcacttttt taccgtttat tttatgtagt tcgataacca ccctgacctc
     6481 actcgatttc ctcggcttcg gtctgccgct cggttcaccg tcactcggtg aactgctgtt
     6541 acaagggaaa aataaccttc aggccccgtg gcttgggatc accgccttct tatcggtggc
     6601 gatattgttg tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa
     6661 taaggcggtg tagcatgacg caaactctgt tagcgattga aaatttgtca gtgggttttc
     6721 gccatcagca aaccgtacgt acagtagtca atgatgtttc actacagatt gcggctggcg
     6781 aaacgctggc gctggtaggt gagtctggtt caggtaagag cgttaccgcg ctgtcaattt
     6841 tacgcctgct cccttgcccg ccggttgaat atctctccgg cgatattcgt tttcatggcg
     6901 aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca
     6961 tgatttttca ggagccgatg gtgtcgttaa atccattaca taccctggaa aaacagcttt
     7021 atgaagtgct ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta
     7081 actgccttga tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc
     7141 agctctccgg cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg
     7201 aattattaat tgccgatgaa ccaaccaccg cgctggacgt ctctgtccag gcgcagattt
     7261 tacagctgtt gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata
     7321 acctcagcat tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg
     7381 tcgagcaaaa taacgctgct acgctatttg cctcacccac tcatccttac acacaaaagc
     7441 tactcaacag tgaaccatct ggcgacccgg tgccattgcc agaacctgcc tcaacgttgc
     7501 tagatgttga acagcttcag gttgccttcc ccatccgcaa agggattttg aagcgcattg
     7561 tggatcataa tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg
     7621 gtttagtggg cgagtccggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga
     7681 ttaattctca tggcagcatc gtctttgacg gtcagccact gcaaaattta aatcgccgcc
     7741 agctgttacc tattcgtcat cgcattcagg tggtatttca ggatccaaac tcctcactca
     7801 acccacgact caacgttttg caaattattg aggaaggctt acgggttcac cagccgacgc
     7861 tttctgccgc acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc
     7921 ctgaaacacg ccaccgttat ccggcggagt tctctggtgg tcagcggcaa cgtattgcga
     7981 ttgccagggc gttaattctt aagccctcgc tgatcattct tgatgaacca acatcatcac
     8041 tcgacaaaac ggtgcaggcg caaatattga cgctattgaa atcattgcaa caaaagcatc
     8101 aactggccta tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg
     8161 ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgccg
     8221 caccgcagca ggagtatacg cgtcagctac tggcgttgag ctgacgctta aaaaggattg
     8281 tagtttgaaa agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca
     8341 aattcgtcct ggcgatttac aaacaggcac ggctcacctt cacactccag caccgaacat
     8401 ggaacttcga tgtcgcttaa cgcctggctt agtttgtaca tcaccgacca tgcattatca
     8461 ccatccgggc taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc
     8521 ggaatcggtt caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt
     8581 cgatggacga ttgagagttg taatggagtc acttattttc cccggaagca catttatttc
     8641 acagtttatt tacatcaact ttaacacatc atcaacaatc cgtctttata cagatcgtaa
     8701 agattgatgc ctcgccagcg tggcctctat ggctctgatt taaataatat ctgcgggcgt
     8761 ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taact
//
LOCUS       DAZFRG010000200         8748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFRG010000200 DAZFRG010000000
VERSION     DAZFRG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.201"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..1733
                     /gene="iucA"
                     /locus_tag="ACHKUC_003786"
     CDS             9..1733
                     /gene="iucA"
                     /locus_tag="ACHKUC_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO6619964.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHIPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1734..2681
                     /gene="iucB"
                     /locus_tag="ACHKUC_003787"
     CDS             1734..2681
                     /gene="iucB"
                     /locus_tag="ACHKUC_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO6619965.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2681..4423
                     /gene="iucC"
                     /locus_tag="ACHKUC_003788"
     CDS             2681..4423
                     /gene="iucC"
                     /locus_tag="ACHKUC_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO6619966.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4420..5753
                     /gene="iucD"
                     /locus_tag="ACHKUC_003789"
                     /pseudo
     CDS             4420..5753
                     /gene="iucD"
                     /locus_tag="ACHKUC_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            5779..7980
                     /gene="iutA"
                     /locus_tag="ACHKUC_003790"
     CDS             5779..7980
                     /gene="iutA"
                     /locus_tag="ACHKUC_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO6619967.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVNDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8531..8674)
                     /locus_tag="ACHKUC_003791"
     CDS             complement(8531..8674)
                     /locus_tag="ACHKUC_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619968.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHRCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2065 a         2285 c         2331 g         2067 t
ORIGIN      
        1 gtttgactat gaccctgccc tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc
       61 tgaatgcact gattcgtgaa accacagact ggaaactgac agaatacccg ccagacgaat
      121 tgctcatccc gctggatgag cagaaatcgc tccatttcag agtggcttat ttctccccaa
      181 cccagcatca ccgctttgca tttcctgcac gtctggtcac ggcatcaggc agttatcctg
      241 tcgactttac cactctctcc cggcttatta ttgataagct acgccatcaa ctgtttctgc
      301 ccgttcccct ctgcgaaact ttccaccagc gcgtgctgga aagccatgtc catacgcaac
      361 aggcaattga tgcccgtcat gactgggccg ctctgcgtga aaaagcgttg aattttggcg
      421 aggctgagca ggcgctgctg acaggacacg ctttccaccc tgcgcctaag tctcatgaac
      481 cgtttaaccg gcgggaggct gaacgctacc tgcctgatat ggcaccccac atcccactgc
      541 ggtggttttc ggtggataaa acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac
      601 agcggctgac gcgatttgcc gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca
      661 accaatggct gttcccgttg cacccgtggc agggggaata tcttttgcag caggggtggt
      721 gccaggcact tgttgctaaa gggctgatta aagacttagg tgaggccggc acgtcgtggc
      781 tgccgaccac ctcttcccgt tccctctact gtgccaccag ccgcgatatg atcaagttct
      841 ccctgagcgt acggctgacc aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg
      901 ggatgcgcct ggcacgcctg gctcaaaccg acggctggca gatgctacag gtccgcttcc
      961 cgactttccg ggtaatgcag gaggatggct gggccgggct gcgcgatctt aacggcaaca
     1021 tcatgcagga aagtctgttt gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc
     1081 agactaacgt actggtctcc ctgactcagg ccgcacctga tggcggtgat tcgctgctgg
     1141 tttcggcggt aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg
     1201 catgggtcga tgcatactgt cagcaagtgc taaagccgct gtttacggct gaagcggatt
     1261 acggcctggt gctgctggcg catcagcaaa atattcttgt ccagatgctt ggggatctgc
     1321 cggtcggatt tatttaccgt gactgtcagg gcagcgcttt tatgcctcac gcgacagact
     1381 ggctcgattc tattggcgag gcgcaggcgg aaaatatttt cacccatgag cagttgctgc
     1441 gctatttccc ttattacctg ctggttaact ccacttttgc tgtgaccgct gcgctggggg
     1501 ctgccgggct ggacagcgaa tcgaatctga tggctcgtgt acgagcatcg ctggctgaag
     1561 tgcgtgatca ggtgactcat aaaacctgtc tcaactacgt gctggaaagt ccgtactgga
     1621 acgtaaaagg taactttttc tgttatctga acgatcataa cgagaacacc attgttgacc
     1681 cttcggtgat ctatttcgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg
     1741 aggcaaacat tattcacagc agatatggac tgcgctgtga aaaactcgac aagcccctga
     1801 atcttggctg gggactggac aatagcgcgg tgttgcactg tcccggggag ctgccgacag
     1861 ggtggctgtg cgatgcgctg gatcagatat ttatcgccgc tccacaactg tcagcagtgg
     1921 ctctcccctg ggccgaatgg cgtgaggagc cacaggcgct gacgcttttt gggcaggtaa
     1981 aaagcgacat tatccatcgt actgccttct ggcagttacc gttatggttg agttcaccgg
     2041 caaaccgggc ctccggtgaa atggtttttg atgcagagcg tgagatttat ttcccgcagc
     2101 gcccaccccg tccacagggt gaggtttacc gccgttacga tccacgtatt cgcaggatgc
     2161 tgagtttccg cattgccgat cccgtttctg atgcagaacg tttcacccgc tggatgaatg
     2221 atccgcgcgt tgagtatttc tgggagcaaa gtggctcact ggaggtacag accgcctatc
     2281 tggagcgcca gctcaccggt aaacatgcat tcccgctgat tgggtgcttc gacgatcggc
     2341 cattcagcta tttcgaaatc tactgggcgg cggaagaccg cattggtcgt cactattcgt
     2401 ggcagccctt tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggggccc
     2461 actatgtgca aagctggctg cgcgggctga cacattacct gctgctggat gagccccgta
     2521 cgcagcgcac agtactggag ccacgcaccg ataacctgcg cctgttccgc catcttgagc
     2581 ctgcgggata ccggacaatt aaagagttcg acttcccaca taagcgctcg cgcatggtga
     2641 tggcggatcg tcatcacttc ttcacggagg tcggtctgta atgaatcaca aggactggga
     2701 ttttgttaac cgtcggctgg tggcaaaaat gttgtctgag atggagtatg agcaggtttt
     2761 ccacgcggaa tctcagggcg atgaccacta ctgcattaac ctgccgggag cacaatggcg
     2821 cttcatcgct gaacgtggta tctggggctg gctgtggatt gatgcccaaa cgttgcgctg
     2881 cacggacgaa ccagtactgg cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat
     2941 gagtgatgcc actgttgcag agcatatgca ggatttgtat gccacgctac tgggcgacct
     3001 gcaactgctg aaggcccgtc gcggactgag tgccagtgac ctgattaatc ttgatgccga
     3061 tcgtctgcaa tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg
     3121 ctggggcaaa gaggcgctgg aacgatatgc gccagagtat accaacacct tcagactgca
     3181 ctggctggcg gtaaaacgtg aacatatgat ctggcgctgc gataacgatc tggatattca
     3241 gcagttgttg actgccgcaa tggatccgca ggagtttaca cgcttcagtc aggtctggca
     3301 ggaaaacgga ctggatcaca actggctgcc gctgccggta catccgtggc agtggcagca
     3361 aaaaatcgct accgacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga
     3421 gtttggcgac cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagtcgccg
     3481 gggcgggctg gatatcaagc tgccgctgac catctacaac acctcatgct accgggggat
     3541 ccctggcaga tacatcgctg ccggaccact ggcttcacgc tggctacaac aggtttttgc
     3601 gaccgacgcc accctagtgc aaagcggcgc agtgatactt ggtgaaccgg ctgcaggcta
     3661 tgtctcccat gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct
     3721 tggtgttatc tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtccggttct
     3781 gatggcaaca ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatataga
     3841 ccgctccggg ctggacgctg aaacctggct tacgcaactg ttccgggtgg tcgtggttcc
     3901 tctgtatcac ctgctttgcc gctacggtgt cgcgcttatt gcacatggac aaaatataac
     3961 tctcgccatg aaagaggggg ttccacagcg tgttctgctg aaagacttcc agggcgatat
     4021 gcgactggtg aaagaagagt tccccgaaat ggactctttg cctcaggagg ttcgtgatgt
     4081 tacatcccgc ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtaac
     4141 agtactgcgt tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta
     4201 tcaactgctg gcagcagtgt tgagtgatta catgaaaaaa catccacaaa tgtcagagcg
     4261 ttttgcgctt ttctcacttt tcaggccaca aatcattcgc gtggtgctga acccggtaaa
     4321 actgacctgg cctgatctgg atggcggcag ccgcatgctg ccgaattacc ttgaggatct
     4381 gcaaaatccg ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt
     4441 attggtgtag ggacagggcc atttaatctc agcattgctg cgttgtcaca tcagatcgaa
     4501 gaactggact gtctcttctt tgatgaacat cctcattttt cctggcatcc gggtatgctg
     4561 gtaccggatt gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc tgttgcacct
     4621 acaaatccct acagttttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt
     4681 acaagcagac tacgtacagt atcccgtgaa gagttttctg actacctccg ctgggctgct
     4741 gaagatatga ataacctgta tttcagtcat accgttgaaa acattgattt cgataaaaaa
     4801 cgtcgattgt ttctggtgca aaccagccag ggagaatatt ttgcccgcaa tatctgcctt
     4861 ggtacaggaa aacaacctta tttaccaccc tgtgtgaagc atatgacaca atcctgtttc
     4921 catgccagtg aaatgaatct tcgtcggccg gaccttagtg gaaaacggat aaccgtggtt
     4981 ggtggaggac agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa
     5041 gcggcggaaa taaactgggt ctcccggcgt aataatttta acgcactgga tgaggctgct
     5101 tttgctgatg agtattttac acctgaatat atttcaggct tctccggact ggaggaagat
     5161 attcgccatc agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct
     5221 ttactgacca tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat
     5281 atccgtctgc tacccagccg ctcggtaaca actctggaaa gtagtggtcc tggctggaag
     5341 ttgctgatgg agcatcatct ggatcagggc agggagagcc tggaaagtga tgtggtgatt
     5401 ttcgccacag gttaccgttc tgcgttgcca caaatacttc cctcactgat gcccctgatc
     5461 accatgcacg ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc
     5521 ccgaaagaga acaacatctt cgtggtcaac gccagtatgc aaacccatgg catcgccgaa
     5581 ccccagctca gcctgatggc atggagatct gcacgtattc ttaatcgcgt aatgggacgt
     5641 gatttattcg atctcagtat gccgcccgcc ctgattcagt ggcgcagcgg cacctaggga
     5701 aaacgcagcc ggaggctgct tctttaactc gctacacagc atctttgggc tgattttttc
     5761 cgcccgtatg gaggaataat gatgataagc aaaaagtata cgctttgggc tctcaaccca
     5821 ctgcttctta ccatgatggc gccagcagtc gctcaacaaa ccgatgatga aacgttcgtg
     5881 gtgtctgcca accgcagcaa tcgcaccgta gcggagatgg cgcaaaccac ctgggttatc
     5941 gaaaacgccg aactggaaca gcagattcag ggcggcaaag agcttaaaga cgcactggct
     6001 cagctgatcc ctggccttga cgtcagcagc cggagccgca ccaactacgg tatgaatgtg
     6061 cgtggccgcc cgctggtcgt gctggttgac ggcgtgcgtc tcaactcttc acgtaccgac
     6121 agccgacaac tggactctat agatcctttt aatatcgacc atattgaagt gatctccggt
     6181 gcgacgtccc tgtacggcgg cggcagtacc ggtggcctga tcaacatcgt gaccaaaaaa
     6241 ggccagccgg aaaccatgat ggagtttgag gctggcacca aaagtggctt tagcagcagt
     6301 aaagatcacg atgaacgcat tgccggagct gtctccggcg gaaatgagca tatctccgga
     6361 cgtctttccg tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc
     6421 ttgcttgata acacccagac cggcctgcag tactccgatc ggctggacat catgggaact
     6481 ggtacgctga acatcgatga atcccggcag cttcagttga tcacacagta ctataaaagc
     6541 cagggcgacg acgattacgg gcttaatctc gggaaaggct tctctgccat cagagggacc
     6601 agcacgccat tcgtcagtaa cgggctgaat tccgaccgta ttcccggcac tgagcggcat
     6661 ttgatcagcc tgcagtactc tgacagcgct tttctgggac aggagctggt cggtcaggtt
     6721 tactaccgcg atgagtcgtt gcgattctac ccgttcccga cggtaaatgc gaacaaacag
     6781 gtgacggctt tctcttcgtc acagcaggac accgaccagt acggcatgaa actgactctg
     6841 aacagcaaac cgatggacgg ctggcaaatc acctgggggc tggatgctga tcatgagcgc
     6901 tttacctcca accagatgtt cttcgacctg gctcaggcaa gcgcttccgg agggctgaac
     6961 aacaagaaga tttacaccac cgggcgctat ccgtcgtatg acatcaccaa cctggcggcc
     7021 ttcctgcaat cagactatga catcaataat ctctttaccc tcaacggtgg cgtacgctat
     7081 cagtacactg aaaacaagat tgatgatttc atcggctacg cgcagcaacg gcagattgcc
     7141 gccgggaagg ctacatccgc cgacgccatt cctggcggct cagtcgatta cgacaacttc
     7201 ctgttcaacg ccggtctgct gatgcacatc accgaacgcc agcaggcatg gctcaacttc
     7261 tcccagggcg tggagctgcc ggacccgggt aaatactatg gtcgcggcat ctatggtgct
     7321 gcagtgaacg gccatcttcc tctaacaaag agtgtgaacg tcaacgacag caagctggaa
     7381 ggcgtgaaag tcgattctta tgagctgggc tggcgcttta ctggcaataa tctgcgcacc
     7441 caaatcgcgg cctactattc gatttctgat aagagcgtgg tggcgaataa agatctgacc
     7501 atcagcgtgg tggacgacaa acgccgtatt tacggcgtgg aaggtgcggt ggactacctg
     7561 attcctgata ctgactggag taccggagtg aacttcaacg tgctgaaaac tgagtcgaaa
     7621 gtgaacggta cctggcagaa atacgatgtg aagacagcaa gcccatcaaa agcgacagcc
     7681 tacattggct gggcaccgga cccgtggagt ctgcgcgtgc agagcaccac ctcctttgac
     7741 gtgagcgacg cgcagggcta caaggtcgat ggctatacca ccgtggatct gctcggcagt
     7801 tatcagcttc cggtgggtac actcagcttc agcattgaaa acctcttcga ccgtgactac
     7861 accactgtct gggggcagcg tgcaccactg tactacagcc cgggttacgg cccagcgtca
     7921 ctgtacgact acaaaggcag gggccgaacc tttggtctga actactctgt gctgttctga
     7981 cggggatgct tttgcaacaa gggtatgctg taaacaacat ggtccttggt ggccatgttg
     8041 tttacagcca gtataatata cagccttaac aagatattcc gtctccggaa tggactgttc
     8101 accactgaat aacattatgt ctgttttatg gatctttgac tgaaatttat cgcatatcct
     8161 catttcattg tccgtctgaa aactaacctt acaagtaagc gtcgtctcag caccgtctgg
     8221 cagatcctga aattcctgag aggatgatct gcgcccaata tcctgagccg gaactttgct
     8281 gcaatgaagg ccaacgaaaa atgggggagt atgttataga gtacttgcta cagggtaaaa
     8341 agaggtacct gtcgctggtt ctccgttcag tttaaccggg agattttctc ctgcgcctgt
     8401 cataaagtcc ggtaatggag attgttaata cgatggtgca cgatgcattg tcagtgcccg
     8461 gcccggagga tgccccgtag ctacatacgg atcagggttg gcaatatcga atggcaggct
     8521 ataggcaaag ttaaagcccc atggagtagc acaaaatatg ccgcacaaag gaaacggtct
     8581 gaataacgca gcgatggaga acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg
     8641 ctaattcgtc gtgaatcact gaacgatgtt ccatgcgaac agatattcga tcatatcgaa
     8701 atattaaaca ttacccctcc ccacattggg caaaaaaaaa caaaaaac
//
LOCUS       DAZFRG010000201         8718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFRG010000201 DAZFRG010000000
VERSION     DAZFRG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.202"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..1109
                     /gene="allD"
                     /locus_tag="ACHKUC_003792"
     CDS             60..1109
                     /gene="allD"
                     /locus_tag="ACHKUC_003792"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO6619969.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1131..2366
                     /gene="allC"
                     /locus_tag="ACHKUC_003793"
     CDS             1131..2366
                     /gene="allC"
                     /locus_tag="ACHKUC_003793"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO6619970.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2377..3162
                     /gene="allE"
                     /locus_tag="ACHKUC_003794"
     CDS             2377..3162
                     /gene="allE"
                     /locus_tag="ACHKUC_003794"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO6619971.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="ACHKUC_003795"
     CDS             complement(3291..4436)
                     /gene="glxK"
                     /locus_tag="ACHKUC_003795"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO6619972.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQA
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4458..5759)
                     /locus_tag="ACHKUC_003796"
     CDS             complement(4458..5759)
                     /locus_tag="ACHKUC_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO6619973.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5815..7176)
                     /gene="allB"
                     /locus_tag="ACHKUC_003797"
     CDS             complement(5815..7176)
                     /gene="allB"
                     /locus_tag="ACHKUC_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO6619974.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7236..8690)
                     /locus_tag="ACHKUC_003798"
     CDS             complement(7236..8690)
                     /locus_tag="ACHKUC_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO6619975.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         2337 a         2307 c         2092 g         1982 t
ORIGIN      
        1 tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga
       61 tgaaaatcag tcgggaaaca ctccaccagc taatcgagaa taaactctgc caggctgggt
      121 taaaacgtga gcacgctgca accgtggctg aagtattggt ttacgccgat gccagaggga
      181 tccactctca tggcgcggtg cgcgttgaat actacgccga acgcatttca aaaggcggca
      241 ccaaccgcga accggagttt cgtcttgagg aaaccggacc gtgctcggca attttacatg
      301 ccgacaatgc cgccggacag gtcgcagcga aaatgggtat ggaacatgcc atcaaaaccg
      361 cccagcaaaa aggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct
      421 catattttgt gcagcaggca gcccgcgccg gattaattgg catttcgctg tgccagtccg
      481 atccaatggt ggtgccgttt ggcggcgcgg aaatttacta cggcactaac ccactggcct
      541 ttgccgcgcc gggagaaggc gatgagatcc ttaccttcga tatggcgact accgtacagg
      601 catggggaaa agtgctcgac gcccgctcgc gcaatatgtc tatcccggat acctgggcag
      661 tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg ctccccgccg
      721 ctgggccgaa agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg
      781 gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacacgcc gggcgtaatt
      841 tggggcaatt acatatagtt attaacccga actttttctc ctccagcgaa ttattccgtc
      901 aacatcttag ccagaccatg cgcgaattaa ataccattac ccccgcgccc ggttttaatc
      961 aggtttatta tcccggacag gatcaggata ttaaacaacg caaagccgcc gtcgaaggca
     1021 tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat
     1081 acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac
     1141 atttccgtca agctatagaa gaaacgctgc cctggctttc ctcttttggc gctgacccaa
     1201 ccggcggtat gacccgttta ctttattcgc cggaatggct ggaaacccag cagcagttta
     1261 aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga tgaagtgggg aatttatacg
     1321 gtcgcctgaa tggcaccgaa tatccacagg aagtggttct gagcggttcg catatcgata
     1381 ccgtggttaa cggcggtaac ctcgacgggc aattcggtgc gctggcggcg tggctggcta
     1441 ttgactggct gaaaacgcaa tacggcgcgc cgttacgtac ggtcgaagtg gtggcgatgg
     1501 cagaagaaga aggcagccgc ttcccgtatg ttttctgggg cagtaaaaat atttttgggc
     1561 tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt tttgtcgatg
     1621 cgatgaaggc ttgcggattt actctgccga acgccccact aactccgcgt caggatatta
     1681 aagcctttgt cgaactgcat atcgaacagg gctgtgtgct ggaaagtaat gggcaatcaa
     1741 ttggcgtggt gaatgcaatt gtcgggcagc gtcgttatac ggtgacgctg aacggtgaat
     1801 caaaccatgc aggcaccacg ccgatgggct atcgtcgtga tacggtttac gctttcagtc
     1861 gcatttgcca tcagtctgtc gaaaaagcga aaaagatggg cgacccgctg gttctgacct
     1921 ttggcaaagt agagccgcgc ccgaatacgg tgaatgtagt gccgggtaaa accacgttca
     1981 ccattgattg tcgtcatacc gacgctaccg tgctgcgcga tttcacccaa cagttagaaa
     2041 acgacatgcg ggcgatttgc gatgaaatgg atattggtat tgatatcgat ttatggatgg
     2101 acgaagaacc cgtgccgatg aataaggagt tggtcgccac cctgacagaa ttgtgtgaaa
     2161 gcgaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg
     2221 cgcctcgcgt gccgacctgc atgattttca ttcccagcat caacgggatc agccataacc
     2281 cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc
     2341 tttatcaact tgcctggcag aaataaggag ttataaatgg gatatttaaa taacgtcacc
     2401 ggttaccgcg atgatttact ggctaaccgt gcgattgtga aacacggtaa tttcgcactg
     2461 ttaacaccag acggtctggt aaaaaatatt attccgggct ttgaaaattg tgacgcgaca
     2521 atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa
     2581 aacggcggca accaacaggg cttcggtggc gaaggcattg aaacgttcct gtatgtgatc
     2641 tctggaaata tcactgccaa agccgaaggg aaaacattta ccttaagtga aggtggctat
     2701 ctttattgcc cgccaggctc cttaatgacg tttgttaacg cccaggccga agacagccaa
     2761 atctttttat ataagcgtcg ctatattccg gtagaaggtc atgcgccgtg gctggtttct
     2821 ggcaatgcca gcgaactgga acgcattcat tatgaaggca tggacgatgt tattctgctg
     2881 gattttctgc ccaaagagtt aggttttgat atgaacatgc atatcctctc ttttgcacca
     2941 ggtgccagcc acggttatat cgaaacccac gttcaggaac acggtgccta tattctttcc
     3001 ggtcaggggg tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc
     3061 tttatgggcg cttattcttt acaggctggt tatggcgtag ggcgtggtga agcgttcagc
     3121 tatatttact cgaaagattg caaccgcgac gtagagattt aagtcatacc actcgcctga
     3181 ttatttttag ctatgttgga ttttgccacg gtttataccg tggctttttt tgtagtcaat
     3241 tcaggtaaaa aaagatgcga tataaatcgc atccgcttta aaggtaatag tcagttttta
     3301 attccctgac ctattttaat ggcgcaggca atatttcgcg cactgttgaa gagattggtt
     3361 tcaccgctgg cgagcacttc ggctaaaggt gccagacgtg gcaaaatgct gaataccgca
     3421 tcaatgccgt actggtgcac cacttccacg ccatcaccca atacgccagc aatcccaatc
     3481 accgggacat taaactgctt cgccaccgac gccacgccca gcggcgcttt accgcctgcc
     3541 gtttgcgagt cgatgcgccc ttccccggtg atcaccagtg ctgcaccctg cactgcctgc
     3601 gcaagattga ccgcattcaa cacaatctca atacccggtt taatatccgc attgaggaat
     3661 accgccgccg caatgcccat accgcccgcc gcgccaccac ccgccatctg gcagacgttg
     3721 atttcagtta gctgttgtaa aacacgggcg taattttgca gcccctgttc cagctcttcg
     3781 accatctccg gcgttgcccc tttttgcggg ccaaacactg ccgccgcgcc gcgtgcccct
     3841 accagcgggt tatcaacgtc acatgctact tcaatatgac agttcgccag acgcggatcg
     3901 cattcattca tctcaatgct tgccacgcgc gctaaattgc cgccgttagc cgccagcgcc
     3961 tgaccgtcgg catcaaggaa acgcacaccg agcgcctgcg ccatgcccat accgccgtcg
     4021 accgtcgcac tgccgccaat gccgagaata atatggcgaa tgccattatc cagcgcatgg
     4081 cgaattaact cccccgtacc aaaactggag gccagcaacg gattacgctt ttcaggcgcg
     4141 accagcatca ggccacttgc tgctgccatc tcaatcaccg ccgttttccc gtcgccggta
     4201 aggccataaa aagcattcac tttttcgccc atcggcccgc agacttcaag cttcacgatg
     4261 ttaccgtccg tcgcggcgac catcgcctcc accgtccctt cgccgccatc cgctatcggc
     4321 aaacagatat agtgcgcatc gggaaatatg gtcgaaaacc cggctttaat tgcctgacaa
     4381 catttttctg cacttaagct ctctttaaaa gagtctggcg caatgacaat cttcattatc
     4441 aatccttttt caacaaatta ttcgatgcgt tcccacggaa taagattatc cataagaaca
     4501 gccagtaaaa ttccgaccag taagccgtta ctgagcagag gacgaagcgt taatggaagg
     4561 tcttgcagat acacaggcgg taatgccatt aaaaatatgc cgacaaataa cggcaatgcg
     4621 agtcgataaa tattgcgagc ggtaaacgtt atttgctggc taaacaccag cgcggaaaag
     4681 agtaaaggca gataagaaac cagcatgacc gcactactca cgggtaaagg gatactgcaa
     4741 aacagtcgcg tgagtgccgg aaccagcgcc accagcaggc aaataacgct gccataaata
     4801 aacgaacgcc gcgtgtaatc gccagtctgg gttaataaac caatggatga aacaaacggt
     4861 gaaaatggaa ttaccgccag cggcacggtt atcagtgtca taaatccggt cgccacaaag
     4921 ctgcgacgat aacgcgtatt ccccgcgccc tgctgcggat aaaaaacatc cgtgccccga
     4981 atcgcaccgt aggtattgct gatatttacc agacctgtaa tcaccgccgt cagaataatc
     5041 cccggcgaca aagcaccgcc actgccgagc gggaaccact gccagtgcaa ctcaccggag
     5101 agcgaatgcg aagaaggaaa gcaaaagtac cacaacaacc agccggttat cgtaccgacc
     5161 agcaggccgt aacgggcaaa acgttgtggc aggaaaataa tcatcgccag taccaggcac
     5221 atcaccgcca ccgagagcgc gaacggcggt aattgaattt taaaattcgg gtcggctatg
     5281 ccaaacggca gcccgagcat acctttgaaa aagatagtgg tcagttgcgc acccagcatc
     5341 aacataaaca agaccatcac cgacggcgta aataaccgtg ccaggcgatg gcctaatccg
     5401 ctaaaaccaa tcaacatcgt cagcacgccg gagagcgcaa tccccaccgc cagactggta
     5461 gcgatatcgt tgatcggtgt cccgcgcgat gcttcgccaa gggtgatagt caggatggtt
     5521 ccccaccaca ggccgcccgg cccctccata atagcgcgac gatgaccgca gaacgcctga
     5581 gcgaagcagg ccagtgcggt agcaagaaaa gcatattgcg tgagcgtaag caggctactt
     5641 tgcggcaact gaaaagcaga aagtagcgta ggaggaacca caaccgtgtt gcaaaaaata
     5701 aagaaaaacc actggaatcc tgataacagg ctttcgcggc ccactgcaaa attgaacata
     5761 aacacacctt aaccggagaa tgcccgccac aaggacgggc attgcagggc cagattactg
     5821 ctgatgttta aggataaatt gacctttcgg cgcaacaggg aagccctgtt cgatatcgta
     5881 aatcacatca ccacgtaaga tggttttcgt gatacgcgcg ccaatagtac ggccaacata
     5941 cgggctgact ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg
     6001 ctgaatgaag acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc
     6061 gaaaatatct gctgcgttag tcgccattaa tttgccgaac attggcagag acattccgcg
     6121 tttctgtacc gcttcatcga acatcacgtc catgcagctt tgcagaccag cgataccgcc
     6181 ccacgctttc atgatgttac cggctttcat ttccggcggg catggagagt ggtcggaaac
     6241 caggcagtct atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc
     6301 gcggatcggc ggtgaacact tcgccagagt accgatttct tcgaactgat cggtatccag
     6361 cacaaagtaa tgcgggcagg attcacaagt aacatcctga ccttcctgac gtgcacgagt
     6421 cacttcctca acaccttccg ggctgctgac gtggcaaacg tgcagacggc aaccagcgac
     6481 tttcgccagg tacagtacgc ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc
     6541 cacatagtca tgggcagtta cgcgaccttc acgcttcgct tcttcgccca gtgcgtcaca
     6601 gatcagcgcg ttttcgcagt gcaccagcac cggctggcct agttcaccca gcttctgcgc
     6661 gcctttgaag aattgccagt cattgacgtc acggaagtcg ttgtcgatac cgcgatcgcc
     6721 acaggtcgca acgaagcatt tgaaaccgac aacacccact tcatccaact catgcagacg
     6781 gtcaatgttg taagacacca ggccaccgag ttgtgccgca tcgatagtca gctttccttt
     6841 agcggcatcg aacttcagtt caattgaggc gcggtcaacc gttgcaggca gctggttgag
     6901 cggcatttcg atcatggtgg tgataccacc ttttgctgct gcgcgagtac cggtttcata
     6961 accttcccag tggctgcgac ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg
     7021 cgaaaccacc agaccagacg catccataac gtcttttgca tcgcccagat cctggccgat
     7081 agcagcaatt tttccgtctt taacggcgat atctacaacg cgagcttcgt tttctaaaat
     7141 aacggtgccg tttttaatga ttaaatcaaa agacataaca aactccttgt aaaatacaga
     7201 ttggttttta actcaatgtt caatatcgtg attaattaac ctatggtttt ttgttctcct
     7261 gttttttctg ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc gacgatgacg
     7321 ccgacaaacc atgaaacacg tgataacggt tccataaagg gaataaactt accgccaaga
     7381 gataaaataa cggccaccag agttactgaa aacgcggtga ggttaaaacc gttatcgtaa
     7441 tatttataat cgccaggtgc ggtgtacagt tcatcaagat taatttgccc acgcatcacc
     7501 acaaaataat gcgccatcat cacaccaatt accggaccaa gcattccgcc gataatatcg
     7561 aggaacagat aaatactgtc ctgattttcc attaatttcc acgggcagat cagcaggctg
     7621 ataatactgg caatcagtac gccgttttta taggtcagtt tcgtcggtgc aatggcggca
     7681 atctgataac cagccggaat aatattaccg gtggcgttag tagagatagt cgtcatcaga
     7741 ataaccagta ccgcaaagaa cgaggcgaac aggctgtccc aacgctgaac aatatccagc
     7801 acgttccagg tgtcagcgcc gtaatgaata ctggctccgg caataataca taccccggcg
     7861 accgcaaaca gaatataggc cacaactaaa cccagcgttt gccccagcgc ctgctcacga
     7921 aacgagtggg cgttttgcgt aaagtcggat gcgctcaccg ccggtgccgc ccagaccgca
     7981 accaccgcgt ttatcaccac cagaaacagg aagccactgt tttctgcttt ctgaataccg
     8041 cccggaatgt agtcaaagat tggaccgatc ccgaccagcg aaatcgccca aatcgccata
     8101 ccgccgaaaa cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttt
     8161 ccgccgccaa aaccaatacc aacgttaacc agccagaaga gtaaaaaagt aattaagccc
     8221 ggtagagaaa ggcctaacag cgtgaaatcg ccaccgagag tcaaaaatcc cggccagatt
     8281 ttgccaatca gaatcaagca ggccagtgac cccgcgtaac attgcaggcc aaaccacata
     8341 atggcagcaa taccgcctct taataatccg ggaaacagtg caccacgtac gccgtaagaa
     8401 gcacgcagga tcatggcaaa cggtacgccg tatttactgc ccgcagcacc gtttaatacc
     8461 attaccgcgg caatgaaaaa ggcactgagg ataattgcca gcataatact aaaggtagac
     8521 aggccgagaa taaaaaagcc gccaaccatc acataattgg gaacgttatg aaccgaaccc
     8581 atccataagg taaaatagtt aaatgttttc caggtccgct gactttgcgt tttcggcagt
     8641 agatcttcgc tatagccgcg ttgctggaat agttttctct gatgttccat aaatccctct
     8701 taatttcacg tatcgaat
//
LOCUS       DAZFRG010000202         8594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFRG010000202 DAZFRG010000000
VERSION     DAZFRG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.203"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..296
                     /gene="trbF"
                     /locus_tag="ACHKUC_003799"
     CDS             <1..296
                     /gene="trbF"
                     /locus_tag="ACHKUC_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO6619976.1"
                     /translation="FVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMYV
                     IPYTLWAFAGGFLIVYVRNPLNELINGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            283..1656
                     /gene="traH"
                     /locus_tag="ACHKUC_003800"
     CDS             283..1656
                     /gene="traH"
                     /locus_tag="ACHKUC_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO6619977.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            1653..4475
                     /gene="traG"
                     /locus_tag="ACHKUC_003801"
     CDS             1653..4475
                     /gene="traG"
                     /locus_tag="ACHKUC_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO6619978.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            4491..4976
                     /locus_tag="ACHKUC_003802"
     CDS             4491..4976
                     /locus_tag="ACHKUC_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO6619979.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            5025..5756
                     /gene="traT"
                     /locus_tag="ACHKUC_003803"
     CDS             5025..5756
                     /gene="traT"
                     /locus_tag="ACHKUC_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO6619980.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            5959..6696
                     /locus_tag="ACHKUC_003804"
     CDS             5959..6696
                     /locus_tag="ACHKUC_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6619981.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKEITGILEKISFPEKSGSNSLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGYHHRHTGYTWSVSVN"
     gene            6747..>8594
                     /gene="traD"
                     /locus_tag="ACHKUC_003805"
     CDS             6747..>8594
                     /gene="traD"
                     /locus_tag="ACHKUC_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO6619982.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGISTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
BASE COUNT         2191 a         1941 c         2321 g         2141 t
ORIGIN      
        1 tttttgtgtt cgttgctgcc ggaacttttt tatgggtgat tatgcatagt atttttgatg
       61 cctgtattga ttcgtggaaa gctgacccgg aattaaataa ttttcgttat atgtggaaca
      121 tcctgatgta tgtcattccg tatacgttat gggcgtttgc aggtggattt cttatcgtat
      181 acgtcaggaa ccctttaaat gaattaataa acggggggat cagaatattc cggctgaaac
      241 gccgtatgcg ccgggagaag aaattaaggg agggtggcaa taatgcttcg cattaaacct
      301 cttctggttc tttgtgccgc tttactgacg gtcacacccg cagcgtcagc ggatgtgaac
      361 agcgacatga atcagttctt taataagctg ggatttgctt ccaataccac acaaccgggc
      421 gtctggcagg ggcaggccgc cgggtatgcc tatggtggct ccctgtatgc ccgcacccag
      481 gtcaaaaata tccagctgat ttccatgacg ctgccggata tcaatgccgg atgtggcggc
      541 atcgacgcct atctcggctc gttcagtttt attaatggcg atcagttgca gcgttttgtt
      601 aagcagatta tgagcaacgc tgccggttac ttttttgacc ttgccctgca ggcaacggtg
      661 ccggaaatca aaaccgcaaa agacttcctg cagaaaatgg caagcgacat taacagtatg
      721 aacctcagct cctgtcaggt ggcgcagggg attgtcggag gattatttcc tcagactcag
      781 gtgtcacagc agaaggtctg tcaggacatt gccggtgaga gcaatatttt tgctgactgg
      841 gccgcgtccc gtcagggctg cacggtcgga ggacagtcat cgtctgttcg tgacaaggcc
      901 agtgacaaag ataaggaacg ggtgctgaag aacatcaata tcatctggga ctcgctgtcc
      961 aaaaacagga tgcttgatgg taacaaagaa ctgaaagaat ttattatgac gctcaccggt
     1021 acgctgattt ttggggaaga cagcgagatt acccccctgc cggcgcgtac cactgaccgc
     1081 gatatcctcc gggcgattat ggagggggga acggcaaaag tttatcactg caacgattcg
     1141 gacaagtgtc tgaaggtggt ggcggatgca aatgtgacca tcgcccggga taaagccctg
     1201 aaatcacaga ttacaaaact gctcaccagt attcagaata aagcggtgag tgatactcca
     1261 ctggatgaca gggaaaaagg cttcatttcc agcaccaccg tccccgtctt taaatatctt
     1321 attgacccgc agatgctcgg tgtgtccagc agcgtggttt accagctgac ggactatatc
     1381 ggctacgaca tcatgctcca gtacattcag gaactgcttc agcaggcccg ggccatgatt
     1441 gccaccggga actatccaca gaccgtgctg gacagtgtgc tggagaatct gaatcaggca
     1501 cagcaacaga ttgcggtttt ccaggggcag gtaaaggtcc agcaggatgc attgctggtt
     1561 gttgaccgtc agatgagcta tatgcgccag cagctttccg cccgcatgct cagccgttac
     1621 cagaacaact atcacttcgg agggagcacg ctgtgaatga agtctatgtg attgccggtg
     1681 gtgagtggtt acgcaataac ctgaacgcca ttgccgcctt tatggggacc cggacgtggg
     1741 attccatcga aaaaattgcg ctcacattgt ctgttctcgc ggtggccgtg atgtgggtac
     1801 agcggcacaa cgtgatggat ttgctgggct gggtggccgt gtttgtgctt atcagcctgc
     1861 tggttaatgt ccgtacatcg gtgcagatta ttgataacag tgacctggtc caggttcacc
     1921 gggtggataa tgtgcctgtc ggactggcga tgccactttc actgacgacc cgtatcgggc
     1981 atgcaatggt ggccagttac gagatgatat tcacccagcc tgacagcgcc acctacagca
     2041 agacaggcat gctgttcggg gcgaacctga ttgtgaaaag caccgatttt ctgtcccgga
     2101 atccggaaat catcaatctg tttcaggact acgtccagaa ctgcgtgctg ggggatattt
     2161 acctgaacca caaatacacg ctggaagacc tgatggcctc cgctgacccc tacacgctga
     2221 ttttttcccg gcccagcccg ctacggggcg tttatgacag caataataat ttcataacct
     2281 gtaaagatgc gtcggtcacg ctgaaagaca ggctgaatct cgatacaaag acgggaggca
     2341 agacctggca ttattatgtg cagcagatat ttggcggcag accggacccg gacctgttat
     2401 tcaggcaact ggtgagtgac agttacagtt atttctacgg ctccagccag tctgccagtc
     2461 atattatgcg ccagaacgtc accatgaatg ccctgaaaga gggtatcacc agtaatgcag
     2521 cccgtaacgg tgataccgcc agcctggtca gtctggccac gacttcgtcg atggagaaac
     2581 agcgtctggc acatgtctcc attggtcatg tgctgatgcg aaacctgccg atggtgcaga
     2641 ccattctggt gggcattacc attggtatct tcccgctcct ggtactggcg gctgtcttca
     2701 acagactgac gctgtctgtt ctgagaggct atgtgtttgc tctgatgtgg ttccagacct
     2761 ggccgttgct gtatgccatt ctgaacagtg ccatgacatt ctatgcaaag cagaatggtg
     2821 caccggtcgt gctctctgaa ctctctcaga tacagctgaa atactccgac ctggcgtcca
     2881 ctgccggata tatatctgtc atgatacccc cgttgtcatg gatgatggta aaaggccttg
     2941 gcgcaggttt ttccagcgtg tacagccatt tcgcttcctc tgctatcagc ccgactgcca
     3001 gtgcggcagg tagtgtggtt gacggtaatt actcctacgg caacatgcag acggaaaacg
     3061 tgaacggctt cagctggagc accaacagca ccacgtcgtt tggtcagatg atgtaccaga
     3121 ccggcagtgg cgcaactgcc acacagaccc gtgacggtaa tatggtgatg gatgcgagcg
     3181 gggcgatgtc ccggttaccg gtcggtatca atgcaacgcg tcagattgca gcggcacaac
     3241 aggaaatggc ccgggaggcg tcgaacagag cagaaagcgc cctgcatggg ttcagcagca
     3301 gtattgccag tgcctggaac tcgctcagcc agtttggttc taaccggggg agcagtgatt
     3361 ctgtcacagg tggtgctgac agcacgatga gcgcacagga ctccatgatg gccagccgta
     3421 tgcgcagtgc agtggaaagc tatgcgaagg cgcataatat cagtaatgag caggcgactc
     3481 aggaactggc atccagaagt acgagagctt ctgccgggat gtatggagat gctcatgctg
     3541 aatggggggt taaacctaaa attcttggtg ttggtggagg attaggcgtt agaggtggtg
     3601 gtcgggctgg tattgattgg gaagataatg atgcgcatac ggcaagtagt aatactcagt
     3661 cgagccataa tgctcgtcat gatatcgatg ccagagctac acaagacttt aaagaggcca
     3721 gcgattattt taccagtcgc aaggtcagtg agtccggcag ccatacggac aataatgctg
     3781 attcccgtgt ggatcagttg tctgcagccc tgaattcagc gaagcagagt tacgaccagt
     3841 acacgacgaa tatgacccgc agccatgaat atgctgaaat ggcatcccgt actgaaagca
     3901 tgagtgggca gatgagtgaa gacctgtcgc aacagtttgc acagtatgta atgaaacacg
     3961 cgccacagga tgcagaagct attctgacaa acaccagttc accggagatt gcagaacgtc
     4021 gtcgggcgat ggcctggtct tttgtgcagg aacaggtaca gcctggtgtt gataatgcct
     4081 ggcgggaatc ccgtggtgat attggtaaag gaatggagag cgtaccttcg ggtggtggca
     4141 gtcaggatat tattgctgac catcaggggc atcaggccat tattgatcaa agaacgcagg
     4201 acagtaatat ccgtaatgat gtaaaacatc aggttgataa tatggtcaca gaatataaag
     4261 ggaatatagg tgatactcag aacagcgtac gtggcgagga aaatattgta agaggacagt
     4321 attctgaact gcagaatcac cataaaacag aggcattatc tcagaataat aaatataatg
     4381 aagaaaagtc ggctcaggaa agaatgcccg gggctgacag tccacaggaa ctgatgaaaa
     4441 gggcaaagga atatcaggat aagtataagc aataatgctg tttttgggag atgataatga
     4501 gatgtttaac ccatattact ctggtaacgg tgatacagtt tatcgcctgt tatctggcta
     4561 gttgggggaa tgccgaaaca atttttatgc tgttcttcat agtactatgg cagggactgt
     4621 ttatatggtt gttctctcaa atacgaaaaa aacggaatgt gtcggatgag tttaagttta
     4681 gtaaaggcgt ttggtatata acgatgccag tatcttcctt actctctcca ttattaagcc
     4741 tgatggtttt tatcattggt acactatacg aattaaggcg agttagtggt tgtgtaagcg
     4801 taagagaatg gatgcaatct caggtaaatg aacaaactaa tgaagatttg catctggatt
     4861 ttgataatat ggattttcat cgtactaatc ctgcaacagg gttacctatg tcaggtattg
     4921 gtgtggatat cggtggtaat tcatatggtt atagttcaaa acgatatgat gagtgaatct
     4981 taatttgtat attatgacgt tttattcaat atgaaggaac attgatgaaa atgaaaaaat
     5041 tgatgatggt tgcactggtc agttccactc tggccctttc agggtgtggt gcgatgagca
     5101 cagcaatcaa gaagcgtaac cttgaggtga agactcagat gagtgagacc atctggcttg
     5161 aacccgccag cgaacgcacg gtatttctgc agatcaaaaa cacgtctgat aaagacatga
     5221 gtgggctgca gggcaaaatt gctgatgctg tgaaagcaaa aggatatcag gtggtgactt
     5281 ctccggataa agcctactac tggattcagg cgaatgtgct gaaggccgat aagatggatc
     5341 tgcgggagtc tcagggatgg ctgaaccgtg gttatgaagg cgcagcagtt ggtgcagcgt
     5401 taggtgccgg tattaccggc tataactcaa attctgccgg tgccacactc ggtgtaggcc
     5461 ttgctgctgg tctggtgggt atggctgcag atgcgatggt ggaagatgtg aactatacca
     5521 tgatcacgga tgtacagatt gcagagcgta ctaaggcaac ggtgacaacg gataatgttg
     5581 ccgccctgcg tcagggcaca tcaggtgcga aaattcagac cagtactgaa acaggtaacc
     5641 agcataaata ccagacccgt gtggtttcaa atgcgaacaa ggttaacctg aaatttgaag
     5701 aggcgaagcc tgttctcgaa gaccaactgg ccaaatcaat cgcaaatatt ctctgaacct
     5761 cgtcaggagg ctgatctgac cggcctcctt gctgacgcct cgcgttgctc gttgtccaac
     5821 cccgggacgt aacaaaataa attttgtcac ttcccggcgt ttccgaaata aacattaaaa
     5881 cactgagcgg ttgtgcaaat tctttgtgcg accgtgaagt gttgccggcg aagccgggat
     5941 cttccggaga aagtcattat gacatcagac aatatcgctg aatatgttgg tgagaataat
     6001 caatttacgg agaagcctgt aactgaatac cccccggtga tacaggataa tacagtgacc
     6061 actaaagact atctgtacag caataatatt gatactgacc ttttagtggt tatcggaggg
     6121 cttattgtta tattgtttct catgatgctt atcatgtact ttattaattc tctcaggcat
     6181 aaggaaataa caggaatact ggaaaaaata tcttttcctg aaaaatccgg gagcaattcg
     6241 cttaaaccgg aagcggtcag ctgtaatttg ttgtccctgt gtgtgctggc accggtattc
     6301 tgtacaccca gaaagctggc agaagtctcc agggagatca tcgtggcgag tgcgcaggaa
     6361 attgcatgga cgccggggag cagtcgtatt gatggccacc aggcggagaa tatcacctgg
     6421 gcgatttcat cgcagtctcc tgccgataag tatgatctca tcaataaaac gtcccgtgtt
     6481 tctcctgaca gtggccgttt ttatgaaccg gacacagttc tggatcaccg tgaacgggta
     6541 cgccttttta ttcagatcca gtttccgcac gggagtgact ggtctgaagt attgcgcctg
     6601 actgacattg ttacagagaa ggtctcttcc ggcaggactg caggcgggta tcaccaccgg
     6661 catacagggt acacctggtc tgtttctgtg aattaacccc acatcatcct tttctgccct
     6721 gttccagatt ttcccggagt ttcatcatga gttttaacgc aaaggatatg acccagggcg
     6781 gtcagattgc gtccatgcgt atccgcatgt tcagccagat cgccaatatc atgctttact
     6841 gcctgtttat ttttttctgg atactcgttg gtctggtttt atgggtaaaa ataagctggc
     6901 agacgtttgt gaacggctgt atttactggt ggtgtaccac gctggaaggc atgcgggatt
     6961 taatcaagtc ccagccggta tatgagatcc agtattacgg caaaaccttc cggatgaacg
     7021 ctgctcaggt actgcatgat aaatatatga tctggtgcgg agagcagcta tggtccgcat
     7081 tcgttctggc tacagttgtg gcactggtta tttgcctgat taccttcttt gttgtctcct
     7141 ggattctggg gcgtcagggt aaacaacaga gcgaaaatga agtcacaggt ggtcgtcagc
     7201 tgacagacaa tccgaaagac gttgcccgga tgctgaaaaa agacggcaag gactccgata
     7261 tccggattgg cgatctgccg attatccggg attccgaaat ccagaacttc tgtctgcacg
     7321 gaacagtgtc gacgggtaag tcagaggtta ttcgacgtct tgccaactat gcccgtaaac
     7381 gtggcgacat ggtggtgatt tatgaccgtt cgtgtgagtt tgttaaaagt tactatgatc
     7441 cctccatcga taagatcctg aatccgctgg atgcccgctg tgccgcctgg gatttgtgga
     7501 aggagtgcct gacacagccg gattttgata atgtcgcgaa cacgctgatc ccgatgggaa
     7561 cgaaagagga cccgttctgg cagggatcag gacgtaccat ttttgcggaa gcggcgtatc
     7621 tgatgcgtaa tgaccccaac cgcagctaca gcaaactggt ggacacactg ctttccatca
     7681 aaatcgaaaa actgcgtacc ttcctgcgta attcaccggc ggccaacctg gtggaagaga
     7741 aaattgagaa aacggcgatt tccatccgtg ctgtgctgac caactacgtg aaagccatcc
     7801 gttacctgca ggggattgag cataacggtg aacccttcac catacgtgac tggatgcggg
     7861 gtgtccggga agatcagaaa aacggctggc tgtttatctc gtcaaatgcc gacactcatg
     7921 cctccctgaa gccggtggtt tccatgtggc tgtccattgc cattcgtggg ctgctggcaa
     7981 tgggagaaaa ccgtaaccgt cgtgtgtggt ttttctgtga tgagttaccc acgttacaca
     8041 aactgccgga cctggtggag atcctgccgg aagcccgtaa gttcggaggc tgttatgtgt
     8101 ttggtatcca gtcctatgcc cagctggaag atatctacgg tgagaaagcg gcggccacgc
     8161 tgtttgatgt gctgaatacc cgtgcctttt tccgctcccc gagccatcag attgcagagt
     8221 ttgctgccgg tgaaattggt gagaaagagc acctgaaagc cagtctgcag tattcctacg
     8281 gtgctgaccc cgtgcgtgac gggatatcaa ccggtaagga gatggagcgc cagacgctgg
     8341 tcagttattc cgacattcag tctctgccgg atctgacctg ttatgtcact ctgcccggac
     8401 cgtatccggc agtaaaactc tcactgaaat atcagacacg accgaaggtc gctccggagt
     8461 ttattccgcg tgacatcaac ccggaaatgg agaaccgtct gagtgccgta cttgccgcaa
     8521 gggaagcaga aggtcgtcag atggccagcc tctttgaacc ggaggttgcc tccggagaag
     8581 atgtgactca ggct
//
LOCUS       DAZFRG010000203         8525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFRG010000203 DAZFRG010000000
VERSION     DAZFRG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.204"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..1485
                     /gene="fucP"
                     /locus_tag="ACHKUC_003806"
     CDS             169..1485
                     /gene="fucP"
                     /locus_tag="ACHKUC_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO6619983.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1518..3293
                     /gene="fucI"
                     /locus_tag="ACHKUC_003807"
     CDS             1518..3293
                     /gene="fucI"
                     /locus_tag="ACHKUC_003807"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO6619984.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEALLNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3402..4820
                     /gene="fucK"
                     /locus_tag="ACHKUC_003808"
     CDS             3402..4820
                     /gene="fucK"
                     /locus_tag="ACHKUC_003808"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6619985.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4822..5244
                     /gene="fucU"
                     /locus_tag="ACHKUC_003809"
     CDS             4822..5244
                     /gene="fucU"
                     /locus_tag="ACHKUC_003809"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO6619986.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5302..6033
                     /gene="fucR"
                     /locus_tag="ACHKUC_003810"
     CDS             5302..6033
                     /gene="fucR"
                     /locus_tag="ACHKUC_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO6619987.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIKLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(6077..7177)
                     /gene="rlmM"
                     /locus_tag="ACHKUC_003811"
     CDS             complement(6077..7177)
                     /gene="rlmM"
                     /locus_tag="ACHKUC_003811"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO6619988.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7170..7565)
                     /locus_tag="ACHKUC_003812"
     CDS             complement(7170..7565)
                     /locus_tag="ACHKUC_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO6619989.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7584..8501)
                     /gene="gcvA"
                     /locus_tag="ACHKUC_003813"
     CDS             complement(7584..8501)
                     /gene="gcvA"
                     /locus_tag="ACHKUC_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO6619990.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         2142 a         2233 c         2098 g         2052 t
ORIGIN      
        1 taagtgtgac cgccgtcata ttacagaccg ttttttattt gaaaatgaat ccatgagttc
       61 atttcagaca ggcaaatatt cactgatatg aagcccgaac tcgctggttt tgcacttttg
      121 aaaacataac cgattacgtg cttaagcttc tgaacctaag aggatgctat gggaaacaca
      181 tcaatacaaa cgcagagtta ccgtgcggta gataaagatg cagggcaaag cagaagttac
      241 attattccat tcgcgctgct gtgctcactg ttttttcttt gggcggtagc caataacctt
      301 aacgacattt tattgcctca attccagcag gcttttacgc tgacaaattt ccaggctggc
      361 ctgatccaat cggcctttta ctttggttat ttcattatcc caatccctgc cgggatattg
      421 atgaaaaaac tcagttataa agcagggatt attaccggat tgtttttata tgccttcggc
      481 gctgcattat tctggcccgc cgcagaaata atgaactaca ccttgttttt agttggccta
      541 tttattattg cagccggatt aggttgtctg gaaactgccg caaacccttt tgttacggta
      601 ttagggccgg aaagcagtgg tcacttccga ttaaatcttg cgcaaacatt taactcattt
      661 ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt tgtctaacgt gccacatcaa
      721 tcgcaagacg ttctcgataa aatgtctcca gagcaattga gtgcgtataa acacagcctg
      781 gtattatcgg tacagacacc ttatatgatc atcgtggcta tcgtgttact ggtcgccctg
      841 ctgatcatgc tgacgaaatt cccggcattg cagagtgata atcacagtga cgccaaacaa
      901 ggatcgttct ccgcatcgct ttctcgcctg gcgcgtattc gccactggcg ctgggcagta
      961 ttagcgcaat tctgctacgt cggcgcacaa acagcctgct ggagctattt gattcgctac
     1021 gctgtagaag aaattccagg tatgacagca ggctttgccg ctaactattt aaccggaacc
     1081 atggtgtgct tctttattgg tcgtttcacc ggtacctggc tcatcagtcg cttcgcacca
     1141 cacaaagtcc tggcagccta cgcattaatc gctatggcac tgtgcctgat ctcagccttc
     1201 gctggcggtc atgtgggttt aatagccctg actttatgca gtgcctttat gtcgattcag
     1261 tacccaacaa tcttctcgct gggcattaag aatctcggcc aggacaccaa atacggttcg
     1321 tccttcatcg ttatgaccat tattggcggc ggtattgtca ctccggtcat ggggtttgtc
     1381 agtgacgcag cgggcaacat ccccactgcc gaactgatcc cggcactctg cttcgcagtc
     1441 atctttatct ttgcccgttt ccgttctcaa acggcaacta actgaacata ttttccgaat
     1501 aaagtgagga atcagtaatg aaaaaaatca gcttaccgaa aattggtatc cgcccggtta
     1561 ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga acaaacaatg aatatggcga
     1621 aagctacggc cgcactgctg accgagaaac tgcgccatgc ctgcggagct gccgtcgagt
     1681 gtgtcatttc cgatacctgt atcgcgggta tggctgaagc cgctgcttgc gaagaaaaat
     1741 tcagcagtca gaatgtaggc ctcaccatta cggtaacgcc ttgctggtgc tatggcagtg
     1801 aaaccatcga catggatcca acccgcccga aagccatttg gggctttaac ggcactgaac
     1861 gccccggcgc tgtttacctg gcagcggctc tggcagctca cagccagaaa ggcatcccgg
     1921 cattctccat ttacggtcat gacgttcagg atgccgatga cacatcgatt cctgctgatg
     1981 ttgaagaaaa actgctgcgc tttgcccgcg ccggtttagc agtcgccagc atgaaaggta
     2041 aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc cggttccatc gttgatcaca
     2101 acttctttga atcctggctg ggaatgaaag tccaggcggt ggatatgacc gaactgcgtc
     2161 gccgtatcga tcagaagatt tacgacgaag ccgaactgga aatggcgctg gcctgggctg
     2221 ataaaaactt ccgctatggc gaagatgaaa ataacaaaca gtatcaacgc aatgccgagc
     2281 aaagccgcgc agttctgcgc gaaagtttac tgatggcgat gtgtatccgc gacatgatgc
     2341 aaggcaacag caaactggcc gatatcggtc gcgtggaaga atcacttggc tacaacgcca
     2401 tcgctgcggg cttccagggg caacgccact ggaccgatca atatcccaat ggcgacaccg
     2461 ccgaagcgct ccttaatagt tcatttgact ggaatggcgt tcgcgaaccc tttgtcgtgg
     2521 cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat gggtcaccag ctcaccggca
     2581 ccgctcaggt atttgccgat gtgcgtacct actggtcacc agaagcaatt gagcgtgtaa
     2641 cggggcataa actggatgga ctggcagaac acggcatcat ccatttgatc aactccggtt
     2701 ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga aggtaacccg acgatgaagc
     2761 cgcactggga aatctctcag caagaggctg acgcttgcct cgccgctacc gaatggtgcc
     2821 cggcgatcca cgaatacttc cgtggcggcg gttactcttc ccgcttcctt accgaaggcg
     2881 gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg cctgggaccg gtactgcaaa
     2941 tcgcggaagg ctggagcgtg gaattgccga aggatgtgca tgacatcctc aacaaacgca
     3001 ccaactcaac ctggccaacc acctggtttg caccgcgcct caccggtaaa gggccgttta
     3061 cggatgtgta ctcggtaatg gcgaactggg gcgctaacca cggagttctg accatcggcc
     3121 acgttggcgc agactttatc actcttgcct ccatgctgcg tatcccggta tgtatgcaca
     3181 acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc cgcgcacggc atggatattg
     3241 aaggccagga ttaccgcgct tgccagaact acggcccgtt gtacaagcgt taatacatta
     3301 ctccctgatg tgatgcccgg tcgctccggc taccgggcct gaacaagcaa gagtggttag
     3361 ccggataagc aatgttatcc ggctatattg caggagcgat tatgaaacaa gaagttatcc
     3421 tggtactcga ctgtggcgcg accaatgtca gggccatcgc ggttaatcgg cagggaaaaa
     3481 ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc gatggaaaac aacacctggc
     3541 accagtggtc tttagacgcc attttgcaac gctttgctga ttgctgtcgg caaatcaata
     3601 gtgaactgac tgattgccac atccgcggta tcgccgtcac cacctttggt gtggatggcg
     3661 ctctggtaga taagcaaggc aatctgctct atccgatcat tagctggaaa tgtccgcgaa
     3721 cagccgcggt aatggacaat attgaacggt tgatctccgc acagcagttg caggctattt
     3781 ctggagtcgg agcctttagt ttcaatacgt tatataagtt ggtgtggttg aaagaaaatc
     3841 atccacaact gctggaacgc gcgcacgcct ggctctttat ttcgtcgctg attaaccacc
     3901 gtttaaccgg cgaattcact actgatatta cgatggccgg aaccagccag atgctggata
     3961 tccagcaacg cgatttcagt ccgcaaattt tacaagccac cggtattcca cgccgactct
     4021 tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct acagaacagc gccgcagcaa
     4081 tgctcggctt acccgttggc ataccggtga tttccgcagg tcacgatacc cagttcgccc
     4141 tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc ttccggtaca tgggaaattt
     4201 taatggtccg cagcgcccag gttgatactt cgctgttaag tcagtacgcc ggttctacct
     4261 gcgaactgga tagccaggca gggttgtata acccaggtat gcaatggctg gcatccggcg
     4321 tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac tccctggcaa atattgattg
     4381 aagaagctcg cctgatcgcg cctggcgcag atggagtgaa aatgcagtgt gatttattgt
     4441 cgtgccagaa cgctggctgg caaggcgtaa cgcttaatac cacgcggggg catttctatc
     4501 gcgcggcgct ggaagggtta accacgcaat tacagcgcaa tctacagatg ctggaaaaaa
     4561 tcgggcactt taaagcctct gaattattgt tagtcggcgg aggaagtcgc aacacattgt
     4621 ggaatcagat taaagccaat atgcttgata ttccggtaaa agttctcgac gatgccgaaa
     4681 cgaccgtcgc aggagctgcg ctgtttggtt ggtatggcgt aggggaattt aacagcccgg
     4741 aagaagcccg tgcacagatt cattatcagt accgttattt ctacccgcaa actgaacctg
     4801 aatttataga ggaggtgtga aatgctgaaa acaatttcgc cgttaatttc tcccgaacta
     4861 ttgaaagtgc tggcagagat ggggcatgga gatgaaatta ttttttccga tgctcacttt
     4921 cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg gcctgttggt gagcgacttg
     4981 ctccaggcga ttatcccgtt atttgagctg gacagttatg caccaccgct ggtgatgatg
     5041 gcggcggtag aaggtgacac tctcgatcct gaagtagaac gacgttatcg taatgcgctt
     5101 tcactacaag ccccctgtcc tgacatcatc cgcatcaatc gttttgcgtt ttatgaacgg
     5161 gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag cgaagtacgg gaatattctt
     5221 ttaaaaaaag gggtaacacc gtaatctcat accggtacgc ccgcatgacg cgggcggtta
     5281 tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc aagcgatagt cgacctgctg
     5341 ctgaaccata ccagcctgac cacggaagct ctctctgaac agctaaaggt cagtaaagaa
     5401 accattcgtc gcgatctcaa tgaattacag acgcagggta aaattctgcg caatcatggg
     5461 cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg atccctttca catccggctg
     5521 aaaagccatt atgcgcataa agcagatatc gcgcgcgagg cgctcgcgtg gattgaagaa
     5581 gggatggtga tagccttaga cgccagttca acctgctggt atctggcacg ccagttgcct
     5641 gacatcaaca tccaggtctt caccaatagc catccgattt gccatgaact cggtaaacgc
     5701 gaacgcatta aactgatcag ttccggcggc acacttgagc gcaaatatgg ctgttacgtc
     5761 aatccctcgc tgatttccca acttaaatcg ctggaaatcg atctgtttat tttttcttgt
     5821 gaagggatcg atagcagcgg cgcactgtgg gactccaatg ccatcaacgc tgattacaaa
     5881 tcaatgttat taaaacgtgc cgcgcaatcg ttgttattga ttgataaaag taaatttaat
     5941 cgttcagggg aagcccgcat cgggcatctg gatgaggtaa cgcacattat ttctgatgag
     6001 cgccaggtta caacttcttt ggtaacagcc tgacggaaag ggtagcaggc cggagacgac
     6061 gccccggcct tacctgttat cgctcgtcgc gacgaccacc caccgcagcc cagatacggc
     6121 ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg ccgcgcctga atctgagcat
     6181 ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag attgtgtgac acttcttcgt
     6241 agcgtttttt catcggcagt ttgagattga agatagtttc acggcaccag ccattaacca
     6301 gccactgcgc cattaatgcc gcaactttcg ccggtttttc aaccatatcg cataccatcc
     6361 aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc ttcccgcagc cacgtcacct
     6421 gcccggtatc catcagactt tgcgccatcg ggccgttgtc gacggaataa acccacatgt
     6481 tgcgcttcac cagttggtag gtccagccgc ctgggcaagc acctaaatcc accgcccaca
     6541 tcccgttcgc caggcgttca tcccactcat ccgcgggaat aaacacatga aatgcctctt
     6601 ccagtttgag cgtggaacga ctcggcgcat ctgccggaaa tttcaggcgc ggaatgccca
     6661 tatagaacgg cgaattattg ctgctgtatg agtaaccagt atagcaacag cctggtgcaa
     6721 taaagaatac atgcacaacc ggacgcttcg gcgtttcata gttcgccagc acccccgcat
     6781 cacgcaaggc agcgcgtagc ggcacggtga atttacggca gaatttcagc aactctttgc
     6841 tttcgttggt atcggcaact tcaacacgca gttcaccgcc cttctctact acgccctgta
     6901 acatgccgac aatgggggta atacgatctt ctggcggcaa atgctgcagg agttccccca
     6961 ctacaaacca ctggcgggca aaaattaatg aactgaacgg cagctcacgg attaacttat
     7021 cgccatcatc aggttgataa cattcataaa tgacataacc cgcattctct ttcacgcggg
     7081 caaaaccgaa aatttcccgc tggccggctt tatcggtaat ttctgcggcg cactcttttt
     7141 caaagcccgg acggcacagc aatacaacct tattcatgac ttacgccctt gcgctttaaa
     7201 cggatagcac caactaacat taacgcccag cccgcaagga agctcacgcc gccaaccggt
     7261 gtgacaaacg cccacaaacg cagatgggac agcgccaggc aataaaggct gccgctgaac
     7321 agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa accagatact gatgcgacgc
     7381 tgcattgcca ccgccagacc taagatcgcc agcgtatgaa acgcctggta ttcgaggccg
     7441 gtctggatcc agcccatctc aacggccccc atggttttac ttaacacatg cgcgccaaaa
     7501 gcgcccagag ctacaaaaat gaagccgcta atggcggcga aaatcagcat aaaacggctg
     7561 gtcatggtcg taccctacgt aaattattgt tcataacgaa agcggaattt ttcttgttca
     7621 gcagcggctt tcgccaggat ccattggcga aaggcggcta ttttacccag ttctgcctgg
     7681 ctgtcatgac aaaccagata aaaagcgttt ttactgacca gaacatcatt aaacgggcaa
     7741 acaagacgtc cggcctcgat ttcagattgc gccatcacgt tatttgcaag cgccactccc
     7801 tgcccgtgga tagccgcttg cagcaccatg gcgctatggc taaaaattgg cccttgctga
     7861 acgttgatat gatttaaccc caactgtcgg gtatatgtct gccagtcacg gcgtgacgca
     7921 tcatgtaata acgtatgttt agccagatct tccggggtct tcaagggttt ttcgccagtc
     7981 agcagtagcg gcgaacacac cggcaataaa tattcggcgt acagtttttc cacccgtagc
     8041 cccggccagt tgccccgacc ataaaatatc gccacatcaa catcatccgc cagcttatct
     8101 tcctgacgat caaccgcctg gattcgaacg tcaattcccg gataagctga attaaagctg
     8161 gaaagtcgcg gaaccaacca atgaatcgcg aaactgggaa gtaaactgac cgtcaacgcc
     8221 cccttagcgc tacgggcctg gagtttacgc gtcgcttcgg ttaattgcga aaatatctct
     8281 ttgatatcga ggaaatagct ttgcccttcc tcggtcagca ggagtgaacg attacggcgg
     8341 cggaacagtt ttagccccag aaaatcctca agagacttga tttgatgact tactgcggcc
     8401 tgggtcacaa aaagctcttc tgctgcgcga gtgaaactta aatggcgtgc tgcggcatca
     8461 aaaactcgta aggcatttag cggtggtaat cgtttagaca tggcttttaa actttgatgt
     8521 taaat
//
LOCUS       DAZFRG010000204         8442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFRG010000204 DAZFRG010000000
VERSION     DAZFRG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.205"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            181..876
                     /gene="aqpZ"
                     /locus_tag="ACHKUC_003814"
     CDS             181..876
                     /gene="aqpZ"
                     /locus_tag="ACHKUC_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO6619991.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1197..2096
                     /gene="lysO"
                     /locus_tag="ACHKUC_003815"
     CDS             1197..2096
                     /gene="lysO"
                     /locus_tag="ACHKUC_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO6619992.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            2240..3892
                     /gene="hcp"
                     /locus_tag="ACHKUC_003816"
                     /gene_synonym="priS"
     CDS             2240..3892
                     /gene="hcp"
                     /locus_tag="ACHKUC_003816"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO6619993.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLMSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3904..4872
                     /gene="hcr"
                     /locus_tag="ACHKUC_003817"
     CDS             3904..4872
                     /gene="hcr"
                     /locus_tag="ACHKUC_003817"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO6619994.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            5005..6723
                     /gene="poxB"
                     /locus_tag="ACHKUC_003818"
     CDS             5005..6723
                     /gene="poxB"
                     /locus_tag="ACHKUC_003818"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO6619995.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERNFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6760..7761
                     /gene="ltaE"
                     /locus_tag="ACHKUC_003819"
     CDS             6760..7761
                     /gene="ltaE"
                     /locus_tag="ACHKUC_003819"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO6619996.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNATWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWHAFLAR"
     gene            7772..>8442
                     /locus_tag="ACHKUC_003820"
     CDS             7772..>8442
                     /locus_tag="ACHKUC_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6619997.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPALLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMA"
BASE COUNT         1869 a         2162 c         2291 g         2120 t
ORIGIN      
        1 aaatgactat atggggcaac ccgctgttgc cctaaatcaa atttactgga tttatttatc
       61 tggcgaatga tttgccttcg tttttaaaat agccgcgcat tatttccgtc gtcacttttt
      121 tacgacgacc ctataaaacg accatatttt tcacagggtc aatttttaat tgaggtggat
      181 atgttcagaa aattagcagc tgaatgtttt ggtactttct ggcttgtttt tggcggctgt
      241 ggtagtgccg tactggccgc aggcttcccg gaattaggca ttggttttgc cggcgtggcg
      301 ttggcgttcg gtctgaccgt tctgacaatg gcctttgctg ttggtcatat ttctggtggt
      361 cattttaacc cggcggtcac tattggttta tgggctggcg gacgtttccc ggcaaaagaa
      421 gtcgttggct acgtaattgc ccaggttgtc ggcggtattg ttgcggcggc gctgctgtat
      481 ttaattgcca gtggtaaaac gggttttgac gcggcagcca gcggttttgc ttctaacggt
      541 tatggcgagc attcaccagg cggttattcc atgctttccg cgctggtagt tgaactggta
      601 ttgagtgcag gtttcctgct ggtgatccac ggcgcaactg acaaattcgc tcctgctggt
      661 tttgcgccaa ttgccattgg tctggcctta accctgattc acttaatcag tattccggtg
      721 actaacactt ccgttaaccc ggcgcgcagt actgcggttg ctatcttcca gggcggctgg
      781 gcattagaac aactgtggtt cttctgggtg gtgccaattg tcggcggtat tatcggtggt
      841 ctgatttacc ggactctact ggaaaaacgc gattaattag cattggcatg ttgtctgccc
      901 tggtggtttt atcttcgacg ttacaaaccg cacgggtgtt ggtggcgtta aaatcgccga
      961 ggcgttcccc gaaggccggg ggggcaggga gcggcggcga ctggccgctc cctgcgccag
     1021 tggctttata tggcattgca catgaaagga gcgcaacaac gctaaacact catatagaat
     1081 taaaaatccc cctctgatgc ataacatttc cttttaattg caactttact cgtccttccg
     1141 ctttcagtag tgtgtcatcg cgcatttcga atcttctttg caaggactgg tttttcatgt
     1201 tttctgggct gttaatcatt ctggttcccc tgattgtggg ttacctcatt ccgcttcgcc
     1261 aaaaagctgc attaagagtt attaatcagc tattaagttg gatggtttac cttattctct
     1321 tttttatggg tatcagtctg gcgtttctcg ataacctcgc cagtaacttg ttggcaattc
     1381 tgcattattc tgccgtcagt attaccgtta ttttactgtg taatattgcc gccctgatgt
     1441 ggctggagcg cggcctgcca tggcgcaacc accatcatca agaaaaactc ccgtcgcgta
     1501 ttgcgatggc gctggagtcg ctaaaactgt gcggcgtagt agtgattggt tttgccattg
     1561 gtctgagtgg actggctttc ttacaacacg cgaccgaagc cagtgaatac accttaattc
     1621 tgctactttt cctcgtcggt attcagttgc gcaataatgg catgacctta aagcagattg
     1681 tccttaatcg ccggggaatg attgtcgccg tggtggtggt tgccagttca ttaattggtg
     1741 gtttaattaa cgcctttatt cttgatcttc ccatcaatac cgcgctggca atggcctccg
     1801 gtttcggctg gtattctctt tccggtattt tattgaccga atcttttggt ccggtaatcg
     1861 ggagcgcggc gttttttaat gatctggccc gtgagctgat tgctattatg ttaatccctg
     1921 ggctgattcg ccgtagccgc tctactgcac tgggcttatg cggtgcgacg tcgatggatt
     1981 tcaccctgcc cgttcttcaa cgtacaggcg ggctggatat ggtcccggcg gcgattgttc
     2041 acggttttat tcttagtctg ttagtgccga ttctcatcgc ctttttctct gcataatacc
     2101 tctctggcgg tagatctctg ccgccaaaat tgcgctaaat caatctccct taaagttgca
     2161 ttaaaaatct cttttatccc cgcgttaagc gtcttaacct taaacatgta tattaaatat
     2221 aactttaaaa ggtgtgatca tgttttgtgt gcaatgtgaa caaactatcc gtactccggc
     2281 aggaaacggc tgctcatacg cgcaggggat gtgtggtaaa acggcggaaa cctctgacct
     2341 tcaggattta ctcatcgcga cgctgcaagg gctttcggcc tgggcagtga aagcacgtga
     2401 atacggcatc atcaaccacg atgtagacag ctttgcgcca cgtgcctttt tctcaaccct
     2461 gaccaacgtt aacttcgatt ctccgcgtat tgtcggctac gctcgtgaag cgattgccct
     2521 gcgcgaggcg ctgaaagcac aatgcctggc tgtagatgcc aacgcccgcg tcgataaccc
     2581 gatggctgac ctgcaactga tgagcgacga tctcggcgag ctgcaacgtc aggcagcaga
     2641 atttacccct aacaaagata aagcggcgat tggcgaaaac attctcggcc tgcgtctgct
     2701 gtgcctgtat ggcctgaaag gtgcggcggc ctatatggaa cacgcgcatg ttctcggtca
     2761 atacgacaac gatatttatg cccagtacca taaaatcatg gcgtggctgg ggacctggcc
     2821 tgccgatatg aacgcgcttc ttgagtgttc aatggaaatc ggccagatga acttcaaagt
     2881 gatgagcatt ctggacgcag gcgaaaccgg taaatacggt cacccgacgc caactcaggt
     2941 caacgtcaaa gcgacggcgg gtaaatgcat tctgatttcc ggtcacgatc tcaaagatct
     3001 ctacaacctg ctggaacaga ccgaaggcac gggcgttaat gtctacaccc acggcgaaat
     3061 gctgcctgcg catggttacc cggagttgcg taaattcaag catctggtcg gtaactacgg
     3121 cagcggctgg cagaatcagc aagtggagtt cgctcgtttc ccaggcccca tcgtgatgac
     3181 ctcgaactgc atcatcgacc caaccgtagg cgcttatgac gatcgtatct ggacccgcag
     3241 catcgttggc tggccgggtg tgcgtcatct ggatggcgaa gatttctctg cagtcattgc
     3301 ccaggcgcaa cagatggcgg gcttcccgta cagcgaaatt ccgcacctga tcaccgtggg
     3361 ctttggtcgc cagacactgc ttggcgcagc cgatacgctg attgatctgg tgagccgtga
     3421 aaaactgcgt catatcttcc tgcttggcgg ctgtgacggc gcacgcggcg agcgtcacta
     3481 cttcaccgat ttcgccacca gcgtgccgga tgactgcctg atcctgaccc tcgcttgcgg
     3541 taaatatcgc tttaataaac ttgagtttgg cgatatcgaa ggtctgccgc gtctggtaga
     3601 tgctggtcag tgtaacgatg cttactcagc aattattctg gcggtaacgc tggcagagaa
     3661 actgggctgc ggagtcaacg atctgccgct gtcgctggtg ctctcctggt ttgaacagaa
     3721 agcgattgtc attctgctga cgctgctttc tctgggtgtg aaaaatatcg tcaccggccc
     3781 gactgcacct ggtttcctga caccagacct gctggcggtg ctgaacgaga aattcggcct
     3841 gcgttctatc accactgttg aagaagacat gaagcaactg ttgagcgcgt aaggagtttg
     3901 tcgatgacga tgccaacgaa tcaatgcccg tggcggatgc aggttcatca cattacgcaa
     3961 gaaacgccgg atgtgtggac gatttccctg atttgccacg attactaccc gtatcgcgcc
     4021 gggcaatatg cgctggtcag cgtgcgtaac tcagcggaaa cgctgcgtgc ttacaccatt
     4081 tcctccacgc caggcgtgag tgaatacatc accctgaccg tgcggcggat tgatgacggt
     4141 gtcggctccc ggtggctgac gcgcgatgta aaacgcggtg attatctctg gctttcggac
     4201 gcgatggggg aatttacctg cgacgataaa gcagaagata aattcctgtt gctggcggca
     4261 ggctgcggcg ttacgccgat tatgtcgatg cgtcgctggc tggcgaagaa ccgtccacag
     4321 gccgatgtgc aggtgatcta caacgtgcgt acgccgcagg atgtgatttt cgccgatgag
     4381 tggcgtaact atccggtaac gctggtggcg gaaaataacg ttaccgaagg ctttatcgct
     4441 ggtcgtctca ctcgcgaact gctggcaggt gtccctgatt tagcctcacg taccgtgatg
     4501 acctgtggcc ctgctccata tatggattgg gtagagcagg aagtgaaagc gctcggcgtg
     4561 acgcgtttct ttaaagagaa attcttcacc ccagtagcgg aagcggcgac cagcggtctg
     4621 aaattcacca aactgcaacc ggcacgagaa ttttacgctc cggttggcac cacgctactg
     4681 gaggcgctgg aaagcaataa cgttccggtt gtcgccgcct gccgtgcggg tgtttgcggc
     4741 tgctgcaaga cgaaagtgat ttccggtgaa tatacggtga gcagcacaat gacgctgacc
     4801 gacgccgaaa tcgctgaagg ttacgtactg gcctgctcct gccatccgca gggggatttg
     4861 gttctcgcat aatcgcctga tgcccgatga tattcctttc atcgggctat ttaaccgtta
     4921 gtgcctcctt tttctcccat cccttccccc tccgtcagat gaactaaact tgttaccgtt
     4981 atcacattca ggagatggag aaccatgaaa caaacggttg cagcttatat cgccaaaaca
     5041 ctcgaatcgg caggcgtgaa acgcatctgg ggagtcacgg gcgactctct gaacggtctt
     5101 agtgacagtc ttaatcgcat gggcaccatc gagtggatgt ccacccggca tgaagaagtg
     5161 gcggcatttg ccgctggcgc tgaagcacaa cttagcggag aactggcggt ctgtgccgga
     5221 tcgtgcggtc ccggcaacct gcacttaatc aacggcctgt tcgattgcca ccgcaatcac
     5281 gttccggtac tggcgattgc cgctcatatt ccctccagcg aaattggcag cggctatttc
     5341 caggaaaccc acccacaaga gctattccgc gaatgtagtc actattgcga gctggtttcc
     5401 agcccagagc agatcccaca agtgctggca attgctatgc gcaaagcggt gcttaaccgt
     5461 ggcgtttccg ttgttgtgtt accgggcgat gtggcgttaa aacctgcgcc agaaggggca
     5521 actacccact ggtatcatgc gccacagccg gtagtaacac cggaagaaga agagttacgc
     5581 aaactggcgc aactgctgcg ttattccagc aatatcgccc tgatgtgtgg cagcggctgt
     5641 gcgggggcgc ataaagagtt agttgagttt gccgggaaaa ttaaagcgcc tatagttcat
     5701 gccctgcgcg gtaaagaaca tgtcgaatac gataatccat atgatgttgg aatgacggga
     5761 ttaatcggct tttcgtcagg tttccatacc atgatgaacg ccgatacgtt agtgctgctc
     5821 ggtactcagt ttccctaccg tgcgttctac ccgaccgatg ccaaaattat tcagattgat
     5881 atcaacccag ccagcatcgg cgcgcatagc aaggtagata tggcgctggt cggcgatatc
     5941 aaatcaaccc tgcgcgcatt gctgccactg gtggaagaaa aaaccgaacg caattttctg
     6001 gataaggcgc tggaagatta ccgcgacgcc cgcaaagggc tggacgattt agctaaaccg
     6061 agcgagaaag ccattcaccc gcaatatctg gcgcagcaaa ttagtcattt tgccgccgat
     6121 gacgctattt tcacctgtga cgttggtacg ccaacggtgt gggcggcacg ttatctaaaa
     6181 atgaacggca agcgtcgcct gttaggttcg tttaaccatg gttcgatggc taacgccatg
     6241 ccgcaggcgc tgggcgcgca ggcaaccgag ccagaacgcc aggtggtcgc catgtgcggc
     6301 gatggcggtt ttagcatgtt gatgggcgat ttcctctcgg tagtgcagat gaagctgcca
     6361 gtgaaaatta tcgtctttaa taacagcgtg ctgggctttg tggcgatgga gatgaaagct
     6421 ggtggctatt tgactgacgg caccgaacta cacgacacca actttgcccg aattgccgaa
     6481 gcctgcggca ttacgggtat tcgtgtagaa aaagcgtctg aaatcgatga agctctgcaa
     6541 cgcgccttct ccatcgacgg tccggtgttg gtggatgtgg tggtcgccaa agaagaatta
     6601 gccattccac cgcagatcaa actcgaacag gccaaaggtt tcagcctgta tatgctgcgc
     6661 gcaatcatca gcgggcgcgg tgatgaagtg atcgaactgg cgaaaacgaa ctggctaagg
     6721 taaaaagggt ggcatttccc gtcataataa ggacatgcca tgattgattt acgcagtgat
     6781 accgttaccc gaccgagccg cgccatgctc gaagcgatga tggccgcccc ggttggggac
     6841 gacgtttacg gagacgaccc taccgttaat gctctgcagg actatgcagc agagctttcc
     6901 ggtaaagaag ccgccatttt tctgccgacc ggcactcagg ccaacctggt cgctctgctc
     6961 agtcactgcg aacgcggcga agagtatatc gtcggtcagg ccgcgcataa ctatctgttt
     7021 gaagccggtg gcgcagcagt gctgggcagt attcagccgc aacccatcga cgcagctgcc
     7081 gacggcacgc taccactgga taaagtggcg atgaaaatca aacctgacga tatccatttc
     7141 gcccgcacca aattactcag tctggaaaac acccacaacg gcaaagtgct gccgcgtgaa
     7201 tacctgaaag aagcatggga atttacccgc gagcgcaatc tggcgctgca cgttgacggc
     7261 gcgcgcattt ttaatgccgt ggtggcttac ggctgcgaac tgaaagagat cacgcaatat
     7321 tgtgactcgt tcaccatttg cctgtcgaaa ggtcttggga cgccagtcgg ttcattactc
     7381 gtcggtaatc gtgattacat taaacgtgcc attcgctggc ggaaaatgac aggtggcggg
     7441 atgcgccagt ccggcattct ggctgccgcc gggatgtatg ccctgaaaaa taacgtcgca
     7501 cgtttgcagg aagaccacga caatgccacc tggatggcgg agcagttgcg tgaagcaggc
     7561 gcggatgtga tgcgtcagga caccaatatg ctgtttgttc gcgtcgggga agaaaatgct
     7621 gccgcgttag gcgaatacat gaaagcgaga aacgtgctga ttaacgcctc gccgattgtc
     7681 cgcctggtga cccatcttga cgtctcgcgt gaacaactgg cggaagttgc cgcccactgg
     7741 catgcgttcc tggcgcgtta aggagagaga cgtgccgcaa cgcattttag ttctcggtgc
     7801 cagtggctac attggtcagc atctggtgcg cacgctcagc cagcaagggc atcagatcct
     7861 ggcggcggca cgtcatgtcg acagacttgc aaaactgcaa ctggctaatg tcagttgcca
     7921 taaagtcgat ctcaactggc cggataacct tccggccctg ttgcagaata ttgatactgt
     7981 ctattttctg gtgcacagca tgggcgaagg cggtgatttt atcgctcagg agcgccaggt
     8041 ggctctcaac gtccgcgatg cgctacgcga agtaccggta aagcaattaa tctttctcag
     8101 ttcattgcag gcgccgcctc atgaacaatc tgatcatctg cgtgctcgtc aggccacggc
     8161 ggatatttta cgtgaagcgg gtgtcccggt gaccgaacta cgtgccggaa ttatcgttgg
     8221 cgcgggttca gcggcattcg aagtcatgcg cgatatggtt tacaacttgc ccgtgttaac
     8281 gccgccacgc tgggttcgtt cacgcaccac gcccatcgcg ctggaaaact tgctgcacta
     8341 tctggtggcg ctgttagatc atccggcctg cgaacatcgc atcttcgaag ccgccggacc
     8401 agaagtgctc agttatcagc aacagtttga acattttatg gc
//
LOCUS       DAZFRG010000205         8438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFRG010000205 DAZFRG010000000
VERSION     DAZFRG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.206"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(155..1207)
                     /gene="fbaB"
                     /locus_tag="ACHKUC_003821"
     CDS             complement(155..1207)
                     /gene="fbaB"
                     /locus_tag="ACHKUC_003821"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO6619998.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1464..2741
                     /locus_tag="ACHKUC_003822"
     CDS             1464..2741
                     /locus_tag="ACHKUC_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO6619999.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2738..3742
                     /locus_tag="ACHKUC_003823"
     CDS             2738..3742
                     /locus_tag="ACHKUC_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6620000.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNTIDPALKAELDTVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3739..4704
                     /locus_tag="ACHKUC_003824"
     CDS             3739..4704
                     /locus_tag="ACHKUC_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6620001.1"
                     /translation="MSGARLHTLLPELTSRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4678..5424)
                     /locus_tag="ACHKUC_003825"
     CDS             complement(4678..5424)
                     /locus_tag="ACHKUC_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6620002.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5476..6294)
                     /locus_tag="ACHKUC_003826"
     CDS             complement(5476..6294)
                     /locus_tag="ACHKUC_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO6620003.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERINWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6359..7159)
                     /gene="thiD"
                     /locus_tag="ACHKUC_003827"
     CDS             complement(6359..7159)
                     /gene="thiD"
                     /locus_tag="ACHKUC_003827"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO6620004.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7156..7944)
                     /gene="thiM"
                     /locus_tag="ACHKUC_003828"
     CDS             complement(7156..7944)
                     /gene="thiM"
                     /locus_tag="ACHKUC_003828"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO6620005.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(7979..8075)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8167..>8438)
                     /gene="rcnR"
                     /locus_tag="ACHKUC_003829"
     CDS             complement(8167..>8438)
                     /gene="rcnR"
                     /locus_tag="ACHKUC_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO6620006.1"
                     /translation="SHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAAI
                     RGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         1918 a         2218 c         2184 g         2118 t
ORIGIN      
        1 atcacaaaaa cgacaatgtg aaaattaatc agaatgacag gcaaaaaaaa cggcctcccg
       61 ggggaagccg taacgaagtg cgcgtgtttt tatgccggat gcaacgtaaa cgccttttcc
      121 gtcctacggg gtggtgctgt tcgtatgagg cgcatcaggc gatagtaatt ttgctatcga
      181 gataaacgtc ctgcacggcg ttaatcagtt tcacgccgtc agccatcgat ttcttgaacg
      241 ccttacgtcc aagaatcagc cccattccgc ctgcgcgttt gttgataacc gcagtacgca
      301 ctgcatcgct gaggtcagtt tcaccgcctg cagcaccgcc ggagtttatc aacccggccc
      361 ggcccatata gcagttagct aactgataac gcaccaggtc aatcgggttt tcactggtca
      421 gtttgctgta gactcgatcg tcggtataac cgtaattaat cgctttatag ccgccgttat
      481 tttccgccat tttttgtttg acgatatctg cacctatggt tgccgccaga tggttcgcct
      541 gaccagtcag gtcggcggaa acatggtaat caacgccatc tttcttaaag gcggagttac
      601 gcaaataggc ccacagcact gtcaccatgc ccagctcgtg cgcacgttca aaagccgcag
      661 aaatttcttc aatctggcga cgtgactctt ccgagccaaa atagatagtc gccccaaccg
      721 ccaccgcgcc catattgaac gcctgctcca cgctggcata cagcgtttga tcgtaggtat
      781 tcgggtaact tagcgtctcg ttgtgattaa gtttgacgag gaatggaatg cgatgcgcgt
      841 aacgccgcga taccgacgcc agcacgccgt aagttgacgc cacacagtta cagcccgctt
      901 cgatcgccag ttcaacaatg tttttcgggt caaagtagag cgggttagcg gcaaatgaag
      961 ctccggcaga gtgctcaacg ccctggtcaa cgggcagaat agaaagatat cctgtgccag
     1021 ccagacgccc ggtgttgtac aacgtctgca tattacgtaa caccgctggc gggcgattat
     1081 tgtcaatcat cacgcggtct acgtagtcat gtccggggag ataaagctgg tcagaaggaa
     1141 tagtcataca acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac tgcgcaatat
     1201 ctgtcattac tatgctcccg taaattccga ttggatatcg gctatggatt gtcctggccc
     1261 gccttttgcg ggcaatcata atcctggtcg ttacgggcga gtttttccat cttttggatg
     1321 ttttttcagc gttttctgtt ggctcgattc atcagaaaaa atgttaccgc ggtcaagttt
     1381 tcaccgcaaa ggtatttaaa aggtattact aagtggtatt gtcatcgcgt accttacatt
     1441 acctgtcatg aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc atgatgtttg
     1501 ttgaatggtt tatctggggc gcgtggtttg tgccattgtg gttgtggtta agtaaaagcg
     1561 gttttagtgc cggagaaatt ggctggtcgt atgcctgtac cgccattgcg gcgatcctgt
     1621 cgccgattct ggttggctcc atcactgacc gctttttctc ggcgcagaaa gtgctggcgg
     1681 tattgatgtt cgctggtgcg gtgctgatgt atttcgctgc gcaacagacc acttttgccg
     1741 ggttcttccc gttactgctg gcctactcgc taacctatat gccgaccatt gcgctgacta
     1801 acagtatcgc ttttgccaac gtgccggatg tggagcgtga tttcccgcgc attcgtgtga
     1861 tgggcactat cggctggatt gcctctggtc tggcatgtgg attcttgccg caaatgctgg
     1921 ggtatgccga tatctcaccg acgaacatcc cgctactgat taccgccgga agttctgctc
     1981 tgctcggtgt gtttgcattt ttcctgcccg acacgccacc aaaaagcacc ggcaaaatgg
     2041 atattaaagt catgctcggc ctggatgcgc tgatcctgct gcgcgataag aacttcctcg
     2101 tctttttctt ctgttcattc ctgtttgcga tgccactggc gttctattac atctttgcca
     2161 acggttacct gaccgaagtt ggcatgaaaa acgccactgg ctggatgacg ctcggccagt
     2221 tctctgaaat cttctttatg ctggcattgc cgtttttcac taaacgcttt ggtatcaaaa
     2281 aggtattgtt gcttggtctg gtcaccgctg cgatccgcta tggcttcttt atttacggta
     2341 gtgcggatga atatttcacc tacgcgttac tgttcctcgg tattttgctg cacggcgtaa
     2401 gttacgattt ttactacgtt accgcttaca tctatgtcga taaaaaagct cccgtgcata
     2461 tgcgtaccgc cgcgcagggg ctgatcacgc tctgctgcca gggcttcggc agtttgctcg
     2521 gctatcgtct tggcggtgtg atgatggaaa agatgttcgc ttatcaggaa ccggtaaacg
     2581 gactgacttt caactgggcc gggatgtgga ctttcggcgc ggtgatgatt gccattatcg
     2641 ccgtgctgtt catgattttt ttccgcgaat ccgacaacga aattacggct atcaaggtcg
     2701 atgatcgcga tattgcgttg acacaagggg aagttaaatg aaaacagaac gtattctcgg
     2761 tgctctttat gggcaggcgt taggggatgc gatggggatg ccctccgagc tttggccgcg
     2821 cagtcgcgtc aaagcacact ttggctggat tgaccgtttt ctgcctgggc caaaggagaa
     2881 taacgcagcc tgttatttta accgcgccga attcaccgac gatacctcga tggcgctgtg
     2941 tctggcggat gcgttactgg aacgtgaagg caagatcgat ccggatctga ttgggcgtaa
     3001 tattctcgac tgggcgctgc gtttcgacgc ctttaacaaa aacgtactag gtccgacctc
     3061 gaagattgcg cttaacgcca ttcgcgacgg taaacccgtt gctgaactgg aaaataacgg
     3121 cgtgaccaac ggcgcagcga tgcgcatctc gccattaggt tgtttgcttc cggcgcgtga
     3181 tgttgattcc tttattgatg atgtggcgct agcctccagc ccgacacata aatccgatct
     3241 ggcggttgcg ggggcggtag tcatcgcatg ggcgatttct cgtgccattg acggagaaag
     3301 ctggtcagca attgttgatt cactgccttc aattgcgcga catgctcaac aaaaacgcat
     3361 cactaccttc agcgcctcac tggctgcacg tctggagatt gcgctgaaaa ttgtgcgcaa
     3421 tgctgacggc accgaatccg ccagcgaaca gctttaccag gtcgttggcg caggtaccag
     3481 tactattgag tccgttccgt gcgccattgc gctggttgaa ctggcacaaa ccgacccgaa
     3541 ccgttgcgcc gtcctgtgcg ctaaccttgg cggcgacaca gacaccatcg gtgctatggc
     3601 gacggcaatt tgcggcgcgt tgcatggcgt taatactatc gatcctgcgt taaaggcgga
     3661 actggataca gtaaatcagc ttgatttcaa ccgctatgcc acggcgctgg cgaagtatcg
     3721 ccaacaacgg gaggcgatat gagcggcgct cgattacaca cgctgctgcc tgaattaacc
     3781 tcgcgtcagc cagttatggt gattggcgcg gcggtcattg atgtgatcgc cgacgcttat
     3841 gccctcccct ggcgtgggtg cgatatcgaa ctgaaacagc agagcgttaa cgttggcggc
     3901 tgcgccctga atattgccgt ggcattaaag cgcctcggca tcgaagcggg taatgccttg
     3961 ccgctcggtc agggggtgtg ggcggagatt attcgcaacc gcatggcaaa agaggactta
     4021 atcagcctga tcgataacgc cgaaggtgat aacggctggt gtctggcgct ggttgagccg
     4081 gatggcgaac gcacttttat gtcgttcagc ggcgtggaaa atcagtggaa tcgccagtgg
     4141 ctggcgcgat taaccgttgc gcctggcagc ctgctctatt tttccggtta tcaactggcc
     4201 tcgccctgcg gcgaattgtt agtggaatgg ctggaagagc tgcaagacgt gacgccattt
     4261 atcgattttg gcccacgtat tggcgatatc ccggatgcat tactggcgcg gatcatggcc
     4321 tgtcgacctt tagtgtcact caatcgtcaa gaggctgaga ttgccgccga acgttttgct
     4381 ctatccgcag agataacaac actaggcaag caatggcagg agaaatttgc cgcgccgttg
     4441 atcgttcgtc tcgataaaga gggcgcatgg tatttcagca acgacgcttc tggctgcatt
     4501 ccggcatttc caacgcaagt tgtagacacc attggggcgg gcgacagtca tgccggtggc
     4561 gtacttgccg ggctggcctc tggtctgcca ctggcggatg ccgtattact gggcaatgca
     4621 gtggcgtcgt gggttgtcgg gcatcggggc ggtgattgtg cgccaacgcg cgaggaacta
     4681 ctcctcgcac acaaaaacgt atagatcgct gcgacagtgg ctgatgctgt actcaatagg
     4741 ccgttgctgt tggtcaagcg ccacttgctt gatcaccagc accggtattt tgctatctaa
     4801 ctgaatatgt gactgaaact cggcatccgg catccgggcg ctaacgcggg aacgcgtacg
     4861 ttgtgggtaa atgtgctggc tgcggaagta gtcataaaga gaaatcccga tggcatcaac
     4921 atcatgaatt aaatgtgccg gaacccacga ttcctcaatc gataccgcct cttcatcgac
     4981 atagcgaata cgcttaagca aaaagacatc acttcccgct tcgaccgcca gctgctgcgc
     5041 gacttcttcg ggacatttca cgacacgctt gttaacccat aacgtatcgg gctttttccc
     5101 gcgcaatacc acctgctgag aaaacccccg cgcttctttc agcgagtatt cgaagatgtt
     5161 gttgatctgc gtgccgtaac cacgcgaacg cgtcaccacg ccctcttctt ccagcgactg
     5221 catcgccttg cgcaccgtaa tgcgcgaaac gccggttaac tgacttaaat cacgctcacc
     5281 gggcaaaata ttgccatgct ccagcacccc gctgcgcacg gcatttttta ccgtttcggc
     5341 aaacttaata taaagcggcg tgttatccgc cgctaaaata cgctcgttca gttgggcaat
     5401 cagctgggta tgcgcttgtt ccatttgtct ttttcctgcc ggggaatctg gtgggagtat
     5461 atcactgctt catatttaag gcgtttcttt aatcccatca acgaatccct gcaactcctc
     5521 caccgtgcca ttaaacacat taaaatccac cggaccatta atgccatcga cctgtccacg
     5581 gtcggaatgc tgccagaagc gccaggccat gccgtcattg tccgggcgac gttgataata
     5641 gtgagccacc caccacggat attcattgaa atagcccgcc agattggtgt gataaaaaac
     5701 ggctcctgag taaataattg gctttttacc cgtacgtttt tcgaccattt ttagccactg
     5761 acttacccgc ttgcgtaatt cttttgccga taattttccc cgttcttcta cgtccagcac
     5821 ggcagggaaa tcgccttgtg agaaatccac cgtttgcaga aataatctcg cctgaactga
     5881 agcagatacc gacggggaaa aataatgata cgccccgcgc agcaggccat tttcgcggct
     5941 tagttgccag ttacgcgaaa aataggggtc caccagcttt tcgccttccg tcgccttaat
     6001 aaaggcaaat tgtaagcgaa taccgttatc gcgcattttt gccacccgct gccagttgat
     6061 ccgctcctgc cagcgcgaaa catctattcc gtgaatggtg taactggcag gaatgcgaat
     6121 agcaaaagat ttgaccggac gataaccgta gaagtggatg tagtctttaa tctgaattgc
     6181 tgtcgagtag aaaaaattca ccgtctgacg cggataaatt gcaacgatgg aaattagccc
     6241 aagagcgcat aataaaacgg tgaatttttt tcgactggtg attctcaact gcattggttt
     6301 catccctgaa tgtcagcacc aaaggctgac aataaccaaa gcagctatag tacggtgctc
     6361 accaccaggc gtggaagtgg tgaaccggac caataccgtg accaacttcc agcgtgtcgg
     6421 cctgggctaa tgccgatgaa agccagcttt ttgcctcctg tacggtgtca gcccagtttg
     6481 tatggcgcgg gcgtagtgca gccaacgccg cagagagcgt acaaccagtg ccgtgagtgt
     6541 ttttggtcat aatgcgctgt gcggtaaacc gttgttcacc ctcgcgggta aacagccagt
     6601 ccgggctttg ctcatcatcc agatgaccac ctttcattag cactgcgcca cagcccatcg
     6661 ccaacagcga tcgcccttgt tccagcattt cctgttcggt gcgcgcgtgt ggcgcgtcga
     6721 gcaaggcggc tgcttcgggc aagtttggtg ttattaatga aacctgtggc aataatcgac
     6781 tgcgtagcgt ggcaactgcc gaaggtgaaa gcagcgggtc gccgcttttt gccagcataa
     6841 cggtgtcgag taccacgttt tggatctgat aacgttgcaa ccgttctgcc accgcttcaa
     6901 caatatcggt ttccgccaac ataccgattt tagtggtgtc gattcgcaca tcgctgaaca
     6961 ccgaatcgag ctgggcggcg acaaaatcag gctcaatgcg atacaccgac tgtacgccac
     7021 gggtattttg cgccactagt gcagtaataa ctgagcaacc ataagcgcca agtgccgaga
     7081 aggttttaag atcggcctga atccccgcac caccactcgg atcagtaccg gcaatcgtca
     7141 gagcgttaat tcgtttcatg cctgcacctc ctgcgtcaat tgccagagcg catcaaggaa
     7201 atgtggaaca aaactacctg gcccctcgct tctggcgact gcgcgttctc cggcttgttt
     7261 catccagtga caggcagatg cgacattttc cagcgtatcg cctggtagcg cacaacaggc
     7321 agcgacaacc gccgataatg cacagccagt tcctaccact ttggtcatta gcggatcgcc
     7381 accgtgaatg ccaacgatac gatgtccatc ggtaacataa tccacctcgc cagtgaccac
     7441 gacgattgcg ccagtttccc gtgccagtgt ttgtgcagcg ggtatcgcgt tagctgcggc
     7501 atcagtggta tccactcccc gtccaccatt agcaacgcca gctaatgcca tgatttccga
     7561 agcattacca cgtatggcgg ttggcttatg ggacagaagt tccacacaaa aacggcggcg
     7621 ataatcgagc gcaccaaccg caaccggatc aagcgtccag ggcgtttgtg atcgtgttgc
     7681 ttgctcaacg gcagcactca tcgcttgcgc tcgtggctgc gtcagtgtgc caacgttaat
     7741 cagcaacgca ctggcgatag ccgcaaactg actggcttct tcggtttcga taaccatcgc
     7801 tggcgatgca ccgagcgcca gcaaggtatt ggcagtaaag gtttgcacca catcgttggt
     7861 catacagtgc acaagagggg aatgttggtg aaaaaggtgt aacgcgtgcg cagattgcgc
     7921 tgaactcagc aggtcgactt gcatagtttg ctcctgccat aacgtgaaga agcaatgacc
     7981 tggtggtccg tgacttccct acgctggcat tatccagatc aggtgatacg ggtatttctc
     8041 agccttcacg cagaagggca ccccgagtcg tttggttgcg atgataaatc gcaaaggagg
     8101 atggtaatgt ccagcgcacg cgttgtaaac gaggaaaaac ggcagtttta caatcgcgtt
     8161 aataaattat ttgatatatg aatccagcac cttcagaacg acatccagat cttcttcacg
     8221 ttttagctca tccccctggt gaacgatgtg ttccgtcaga tgacctttaa tcacttcccg
     8281 catcagaccg ttcaccgcgc cacggatagc agcaatctgt tgtaaaactg cagcgcattc
     8341 gtgcggctcg tcgagcattt tcttgagcgc cacgacctgg ccctgaatct tactggcacg
     8401 cgctttcagt ttctgtttat cacggattgt atgagaca
//
LOCUS       DAZFRG010000206         8373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFRG010000206 DAZFRG010000000
VERSION     DAZFRG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.207"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..2451
                     /gene="ppsA"
                     /locus_tag="ACHKUC_003830"
     CDS             73..2451
                     /gene="ppsA"
                     /locus_tag="ACHKUC_003830"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO6620007.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPLEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2508..4154)
                     /gene="fadK"
                     /locus_tag="ACHKUC_003831"
     CDS             complement(2508..4154)
                     /gene="fadK"
                     /locus_tag="ACHKUC_003831"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO6620008.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEDGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4211..4504)
                     /locus_tag="ACHKUC_003832"
     CDS             complement(4211..4504)
                     /locus_tag="ACHKUC_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6620009.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4501..5790)
                     /locus_tag="ACHKUC_003833"
     CDS             complement(4501..5790)
                     /locus_tag="ACHKUC_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6620010.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKLPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5845..6783)
                     /locus_tag="ACHKUC_003834"
     CDS             complement(5845..6783)
                     /locus_tag="ACHKUC_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO6620011.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(6803..7567)
                     /locus_tag="ACHKUC_003835"
     CDS             complement(6803..7567)
                     /locus_tag="ACHKUC_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO6620012.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGELLQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTKRKHIILDNDSPEAIAELAEHLKKALN"
     gene            7883..>8373
                     /locus_tag="ACHKUC_003836"
     CDS             7883..>8373
                     /locus_tag="ACHKUC_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO6620013.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYE"
BASE COUNT         1988 a         2170 c         2181 g         2034 t
ORIGIN      
        1 gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc aaaccgttca tttatcacaa
       61 aaggattgtt cgatgtccaa caatggctcg tcaccgctgg tgctttggta taaccaactc
      121 ggcatgaatg atgtagacag ggttgggggc aaaaatgcct ccctgggtga aatgattact
      181 aacctttccg gaatgggtgt ttccgttccg aatggtttcg ccacaaccgc cgacgcgttt
      241 aaccagtttc tggaccaaag cggcgtaaac cagcgcattt atgaactgct ggataaaacg
      301 gatattgacg atgttaccca gcttgcgaaa gcgggcgcgc aaatccgcca gtggattatc
      361 gacactccct tccagcctga gctggaaaac gccatccgcg aagcctatgc acagctttcc
      421 gccgatgacg aaaacgcttc gtttgcggtg cgttcctccg ctactgcaga agatatgccg
      481 gacgcttctt ttgccggtca gcaggaaact ttcctcaacg ttcagggttt tgacgccgtt
      541 ctcgtggcag tgaaacatgt atttgcttct ctgtttaacg atcgcgccat ctcttatcgt
      601 gtgcaccagg gttacgatca ccgtggcgtg gcgctctccg ccggtgttca gcggatggtg
      661 cgctccgacc tcgcatcttc tggcgtgatg ttctccattg ataccgaatc cggctttgac
      721 caggtggtgt ttatcacttc cgcatggggc cttggtgaaa tggtcgtgca gggtgcggtt
      781 aacccggatg agttttatgt gcataaaccg acacttgcgg cgaatcgccc ggctatcgtg
      841 cgccgcacca tggggtcgaa aaaaatccgc atggtttacg cgccgaccca ggagcacggc
      901 aagcaggtta aaatcgaaga cgtaccgctg gaacagcgtg acatcttctc gctgaccaac
      961 gaagaagtgc aggaactggc aaaacaggcc gtacaaattg agaaacacta cggtcgcccg
     1021 atggatattg agtgggcgaa agatggccac accggcaaac tgttcattgt gcaggcgcgt
     1081 ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt atacgctgca ttcacagggt
     1141 aagattatcg ccgaaggccg tgctatcggt catcgcatcg gcgcgggtcc ggtgaaagtc
     1201 atccatgaca tcagcgaaat gaaccgcatc gaacctggcg acgtgctggt tactgacatg
     1261 accgacccgg actgggaacc gatcatgaag aaagcatctg ccatcgtcac caaccgtggc
     1321 ggtcgtacct gtcacgcggc gatcatcgct cgtgaactcg gcattccggc ggtagtgggc
     1381 tgtggtgatg caacagaacg gatgaaagac ggcgagaacg tcactgtttc ttgtgccgaa
     1441 ggtgataccg gttacgtcta tgcggagttg ctggaattta gcgtgaaaag ctccagcgta
     1501 gaaacgatgc cggacctgcc gttgaaggtg atgatgaacg tcggtaaccc ggaccgtgct
     1561 ttcgacttcg cctgcctgcc gaacgaaggc gtgggccttg cgcgtctgga atttatcatc
     1621 aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt ttgacgatca ggaaccgcag
     1681 ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt ctccgcgtga attttacgtt
     1741 ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt tttatccgaa gcgcgtcatt
     1801 gtccgtcttt ctgattttaa atcgaacgaa tatgccaatc tggtcggtgg tgagcgttac
     1861 gagccagatg aagagaaccc gatgctcggc ttccgtggcg cgggccgcta tgtttccgac
     1921 agcttccgcg actgcttcgc gctggagtgt gaagcagtga aacgtgtgcg caacgacatg
     1981 gggctgacta acgttgagat catgatcccg ttcgtgcgta ccgttgatca ggcgaaagcg
     2041 gtggttgagg aactggcgcg tcaggggctg aaacgtggcg agaacgggct gaaaatcatc
     2101 atgatgtgtg aaattccgtc caacgccttg ctggcagagc agttcctgga atatttcgac
     2161 ggcttctcaa ttggctcaaa cgacatgacg cagctggcgc tcggtctgga tcgtgactcc
     2221 ggcgtggtgt ccgaactgtt cgatgagcgc aacgatgcgg tgaaagcact gctgtcgatg
     2281 gcgattcgtg ccgcgaagaa acagggcaaa tatgtcggga tttgcggtca gggtccatcc
     2341 gaccacgaag actttgctgc atggttgatg gaagagggga tcgatagcct gtctctgaac
     2401 ccggacaccg tggtgcaaac ctggttaagc ctggctgaac tgaagaaata aaataaatac
     2461 ccggcggcgt tttgtcgccg ggtttatgtg atccccgaag atgaaactta ttcaatctct
     2521 tcacagacat cctgagttaa tcgccgcata atatctttcc ttaacaaaaa cttttgtatt
     2581 ttacctgaga cagtgcgcgg tagtttttcg attaccacga tatgttcagg atatttatat
     2641 tttgcgaccc gtttacggct aaaaaaagcc accacctctt ccagcgataa tgaatgatgc
     2701 ggcgctttca gcacgacata agcgcatgaa cgttcaccta aacgttcatc gggcatcgca
     2761 accacacagg catcgtgaat tttaggatgc tgcaataaaa tatcttctac ttcacggctg
     2821 ctaatatttt cgccgccgcg gacaataata tctttcttgc gcccggttat ttttatatag
     2881 ccatcctcat ccatgcggca gagatcgccg ctgtaatacc agccttcttc atccagcgca
     2941 cgggcggtta attcaggttc atcaaaatac cccataaaca cattggggcc acgcgaggct
     3001 tcttcacctt cgcaacctgg cggtaaagtc ttgcgtgcgt catcgaccac tttaatctct
     3061 acacctgcgg cagcgtaacc gtcggtgtgc ataaagcgcg gcaaaggatc gtcgagattc
     3121 accaccgcat gcggcgaact ttctgtggaa ccataaacac ttaataattt aatgccgcgc
     3181 tgctggcatt ctcgcgccac ttttttgggg attgtggtac ctccgcaaag aaagaagcgc
     3241 agcgctgaaa ggtctgcggg ttgtttctct agtaaattca aaagatcata gacaaacggc
     3301 gttgcgccga gcatacaggt gcaacgctgc tgctcaagca gcgcgagaca cgcatcagga
     3361 gtgaaaatat ctaacaacac gctgcgagca ccaatcaaaa aaggtgctgt tacgccatgc
     3421 agaaagcccg ttgcgtgacc aagtggcgca ggcatcataa agacatcctg ccaggtcaga
     3481 tttagccgcg cgcaataagc ccgctcactg gcgagaatat tgttatgcgt tagcatcaca
     3541 ccctttggca gaccctcggt tccggaggta aatagcacag cggctaattc atcgccgtgg
     3601 accgttatcg ccgtggtcag aggagtattg tcggcgagaa tctgacttaa tgagagggaa
     3661 gaggtggcgg gagccagttt gtccacgccg acaatttgtt gtagttgtgg aagctgattt
     3721 tgcagcggca ggattaaatc taccggacgc gtttgtttaa acaacgtcgg tgcaaagaac
     3781 atttttgcct gacacttatt gagcacccac accagttctg cttcccgcca ggaaggtaac
     3841 agcggcacgg aaaccgcacc ggttttcagg caggcaagat agataacggt aaattcacac
     3901 cagcctggca gttgaaatgc gatgcgatcg cctgattcaa tacccttcgc taacatccag
     3961 tttgccagac agctcgcggc gtgatcgagc gcgctatagg tgtacgttgc accatgattg
     4021 tcgaccacgg caattttgtc tggcatcgca cgagcggtct gctgccagta atcggccagt
     4081 gaagcatcgc cccataaccc ttgctgacga tacgccgcac gacgttgttc gttgaacgtt
     4141 aatgtcactt tcattttgga ctctcgaaac agaacatcag gcccgagatg cgggcctgtg
     4201 ggatgcatga ttagccgtag cgaaattcaa taccgaaggt gcctgcggga tattgccact
     4261 gttcgagaat agtgttgccg cacagcaccc gacaggtgcc gcactccaga caaccggcgg
     4321 aatcgaaatg aatgtttcct gcgtcatcct gcttataaag tccggcaggg caagctttca
     4381 ttaatttacg gaattcattg atatcgggat ttgccgccaa aatgatatgc gggtggccct
     4441 catcaacatg gaatttattg acgcctaatt tgatgtcgac gttaaccgta gcgttctggc
     4501 tcatagcgcg gttgctccct taatgccatc tttcagcaag ttgatcagcc caattttctt
     4561 cgcgtgtccc atgatcattt tgcgtaccgg ctggtttggt ttgccgtcaa tggtgaacat
     4621 atcgttcatg atgtcggcga ccattcgtgg gtattggcta aacaggcgcg ggttttccat
     4681 cagcgccggg agcttgcgga aatgctgcat atcgcgcata acacaacttt gttccagctc
     4741 acgtttgtat tgcgccagac tgctggcgga gaaatccgcg cgttctttgg cggcgatcac
     4801 cgttgtggcg gcagcctgag ctgatgcaat ggctaaatcc atgccgcgaa ctgtaaaacc
     4861 caaattcagg cagaagcctg cggcgtcacc aacgatcatc acgccatcgt taaccagctg
     4921 cggcaccatt gccagaccgc cctccggcac catatgcgcg gaatattcaa gcagtttgcc
     4981 gccgctaatc agcgggcgaa tggcggggtg ttgtttaaaa tcttccagca tttgcggcac
     5041 gcttttttgc gcatgggcga tatcacccaa tccacaaacc agccccaacg atacggaatc
     5101 cttgttggta tagaggaatc cgccgcccat caggccgtca gaaggggaac cggcaaacag
     5161 ccaggcggca ccttcctcgc ccgtaacatt aaagcgatcg ttgatctgtt ctggtgtcag
     5221 gcctataacc tctttaacac caacagcgta atgatgcgga ttggaagcgg aaaccattcc
     5281 cagcgagcgg ccaagcatcg agttaacgcc atcagccaga atcaccacat tcgcttcgag
     5341 aatatcatcc ccggcctgga cgccagtgac cttgtttcct tcacgaacca gcgcatcgac
     5401 gcgtacgccc gggataaact gcgcgcccgc ctgctcggct tgttccatca accacgggtc
     5461 cagacgatta cgcaatacgg tataagacgc gtgttgcgga acatctggtt gctcgcggtg
     5521 aaaatcgagg gtaacggcgc tttcttcggt taagaaggag attttctcgc gtgtgacctt
     5581 gcgttctacc ggcgctgatg ctgcaaagcc tggaataatt gcttcaagtg tgtgggcata
     5641 aagacgcccg ccggtcatgt ttttacatcc ggcactgtcg ccgcgttcta tcaccaggac
     5701 atccagcccg gctcgcgcca tgaccagtgc ggcaacgcta ccggcaacgc ccgcaccgac
     5761 cacaatggca tcaaatttgt catccgacat gcgttatgct cccttcccgc tgcgtttaca
     5821 gcgggagatg tggttgaaag ggaattagcg attcaactgg ctaatcaggg cagggacgac
     5881 tttgtagata tcgcccacca gaccgtagtc ggcatagttg aagattggcg catttttatc
     5941 tttattaatg gcgacaatca cttttgcgcc gttgccgcca accatatgct ggatctgccc
     6001 ggagatcccc agcgtcaggt agagatcgga tttcagcaac acgccggaga caccgatata
     6061 acgttcacgc tccatccagt tctcgccttc ggcaattgga cgtgaacagc cgacttcagc
     6121 attcagcact gccgccagtt cgtggaccat tttcaggtca tcctgcgccg ccagaccacg
     6181 accgacgcca accacacgtt ttgctttgct caggtccacg ctgcttgccg ctttcgcacg
     6241 gcgttcgcga cagagaattt catgacgcgg ggcaacataa ggtaccgttt ctgtcgggca
     6301 ctgatgagag atatcacggg tgcacggctc ctgaacaccg ggtgcaaggg taatgatcgc
     6361 cagcgggctg ttgatctttt cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc
     6421 gcaaatgtga ccatcgacaa tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag
     6481 ttgcacactt aaccgtgcgg ccagagcttt accacgtttc gtcgcggcca acagcaacat
     6541 agcggggtgt ttatctttca gcagggcagc aatgctttcg gcgtaatttt cagtacgttg
     6601 cagcgcgtcg ttttgctcaa gaacataaat gcattttggc ccataaggca taaccgcctg
     6661 cgcctgctcg gtattttgta caatggcata cacctgttgg ccccattgct gagcgccgcc
     6721 aaacagttca gcataacgtt caggattatc gctaaagacc cagacgctgt ttaattgact
     6781 catcgtttct tctccgtagg cttcagttca gggctttttt cagatgttcc gccagctcgg
     6841 caatggcctc cggcgaatcg ttatcgagaa tgatgtgctt acgttttgtt tgcggcggta
     6901 cgcgaatacc ggtcagttca gcaagtggtg cgctctggct ccagccaata tcacttgcct
     6961 gccactgatt taccggtttt ttacccgcgc cgaggatggc tttcatcgaa ggaatacgtg
     7021 gtacgttaat atcggaggtg acgcagagca cggctggaac agagagttca ataacttcaa
     7081 catcatcttc aagcgtgcgt tcaatcacca gtgtattgcc ctgacgctga atagcactca
     7141 ctgcattaat caccggaagt tgcagaagtt ctccgaccag caagccaacc tgctgggcat
     7201 aaaggtcgcc ggaaccttca ccaaagatca gtaaatcgaa gccgatcttt tcaactgctg
     7261 tcgccagcgc ctttgcggta tcgagaggca gtgcatgctc aagttgtgca tcctgcacca
     7321 tatacaggct gtgcggcccg cgggatagca cgtctttgcg cactttcgag ttctgcaaca
     7381 aagagccgcc gacggtcagc gcggctatct catcgtcatc tgttgcaagc tggcttgcag
     7441 cttcaatggc attgagatcg aactggctga ttttggcgtc ggcattgtcg aaattcaggg
     7501 tgtattctgg agtgacaaca atgtcctgtt cttcaggcac cagcttaaag caggttatta
     7561 ttttcatgat ttctcctgtg agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg
     7621 cctgaaaata gtgtgcgata gcggtgcggg gttttcgacg gagcaagctg ttttccttgc
     7681 caacaatctt gcaaaaaaaa tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag
     7741 ttattccatg accgtgcaat cttgcattca gtgtaaataa tcttgtttag ctctcgctaa
     7801 acgcggttgt ataaccgcaa tacacgccgt attttatccg gcatatattg tttttgtttg
     7861 ctcatggaat agggaggttg tcatgtatca acgctgtttt gataatgcca gtgaaacgct
     7921 gtttgtcgcc ggtaaaacgc cacggctttc acgtttcgca tttagcgatg atccaaaatg
     7981 ggagtctgga catcacgttc atgacaatga aaccgagctg atttacgtca agaaaggggt
     8041 agcaaggttt accatcgatt cttcgttata tgtcgcgcat gccgatgaca ttgtggtgat
     8101 agaacgtggc aggttgcatg cggtggcctc tgacgttaac gatccggcaa cgacgtgtac
     8161 ctgtgcgctg tacggctttc agtttcaggg ggctgaggaa aatcagctac tgcaaccgca
     8221 ttcttgcccg gtaattgccg caggacaggg aaaagaagtc attaaaacct tatttaatga
     8281 gctaagtgtg attttgccgc aaagtaaaaa tagccaaaca tcttcgttat gggacgcatt
     8341 tgcctatacg ttagcaattc tttactacga aaa
//
LOCUS       DAZFRG010000207         8151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFRG010000207 DAZFRG010000000
VERSION     DAZFRG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.208"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            238..1221
                     /locus_tag="ACHKUC_003837"
     CDS             238..1221
                     /locus_tag="ACHKUC_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO6620014.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1293..2441
                     /locus_tag="ACHKUC_003838"
     CDS             1293..2441
                     /locus_tag="ACHKUC_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HFO6620015.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2465..4141
                     /locus_tag="ACHKUC_003839"
     CDS             2465..4141
                     /locus_tag="ACHKUC_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO6620016.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4151..4891
                     /gene="kdsB"
                     /locus_tag="ACHKUC_003840"
     CDS             4151..4891
                     /gene="kdsB"
                     /locus_tag="ACHKUC_003840"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6620017.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAAEAAEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4888..6915
                     /locus_tag="ACHKUC_003841"
     CDS             4888..6915
                     /locus_tag="ACHKUC_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO6620018.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFAALRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWAP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWLAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERTPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTIADLIYQALIVYPTYI
                     HPTLLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            6950..8119
                     /locus_tag="ACHKUC_003842"
     CDS             6950..8119
                     /locus_tag="ACHKUC_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO6620019.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSSYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERILYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAVYY"
BASE COUNT         2096 a         1972 c         2102 g         1981 t
ORIGIN      
        1 taaataggtg ggcaaaattt ggttcccttt ctcgcctgta atattgcagc gtcttcgcac
       61 ctccatgaga cattgcgact taataagaag gtgataagtc ctgcatataa gcatggactg
      121 accatggttt tatattcatt aaaaattttg ttacaaccca ttgatttagc ataaataaat
      181 tatagtgggt tcgggtttgt tgtgactgtg gcattatttc cgtgcaaagg agctgatatg
      241 tctgaaagac atttacctga tgaccagagc agtactatcg atccatatct aattacctct
      301 gttcgccaga ctctggcaga acaaagcgca gcattacaaa acttgtctaa acaactggat
      361 tccgggcagt accagcgtgt ccttaatttg ataatgaact gtaaagggca cgttattctt
      421 tcgggaatgg gtaaatcagg gcatgtcggt cgtaaaatgt cagcgacgct ggcctctacg
      481 ggtacgccta gtttctttat tcatcctgca gaagctttcc atggcgatct gggcatgatt
      541 acgccttacg atcttctgat ccttatttct gccagcggtg aaacggatga aatcctcaag
      601 ctagttcctt cactgaaaaa tttcggcaac cgaattatcg ccattaccaa taatggaaat
      661 tccacgctgg cgaaaaatgc tgatgccgtg ctggaactcc acatggcgaa tgaaacctgc
      721 ccgaataatc ttgcaccaac aacgtctacc acgctgacga tggcgatcgg cgatgcgctg
      781 gcgattgcca tgatccacca acgcaaattt atgccgaatg attttgcgcg ctatcacccg
      841 ggcggttcat taggtcgtcg cctgctgacc cgcgttgctg atgtcatgca gcatgatgtt
      901 cctgcggtac agctggatgc gtcatttaaa accgtgattc aacgtatcac cagcggatgc
      961 cagggaatgg tgatggtaga agacgcagaa ggtgggctag cgggcattat caccgacggt
     1021 gacctgcgtc gctttatgga aaaagaggat tctctgacat ccgccacggc tgcgcagatg
     1081 atgacacgtg aaccgctgac gctaccggaa gacaccatga tcattgaagc ggaagaaaaa
     1141 atgcaaaagc accgcgtctc aacgttattg gtgaccaaca aggcaaataa agtcactggc
     1201 cttgtgcgca ttttcgacta attaagcaac ggggtgcgag aggttagtct ctcattgagt
     1261 ttgcgcccca ctgaaacttt gataatcgtt acatgttgat aaaagtgaag tctgccgtat
     1321 cctggatgcg tgctcgtctg tctgccatct cactggcaga tatccaaaaa cacctggcga
     1381 aaatcatcat tctggcaccg atggcggtgc tgctgatcta tctggctatc ttcagccagc
     1441 ctcgctatat gagcgagtcg aaagtcgcca ttaaacgctc ggatgattta aacagcggca
     1501 gcctgaattt tggtctgctt ctgggtgcct ctaaccccag ttccgcagaa gatgcgttgt
     1561 atctgaaaga gtacatcaac tcgccggata tgctggcggc gctggataag caactaaatt
     1621 ttcgtgaagc gtttagccac agcgggctcg attttcttaa tcatcttagc aaggatgaaa
     1681 ccgcagaagg cttcctgaag tactacaagg accgtatcaa cgtctcgtat gacgataaaa
     1741 ccggattact gaatattcag acgcagggct ttagcccgga gtttgcgctt aagtttaacc
     1801 aaaccgtgct gaaagagtca gagcgcttta tcaatgagat gtcacatcgc atcgcgcgtg
     1861 accagcttgc ctttgcagaa acggagatgg aaaaggcacg ccagcgtctg gacgccagca
     1921 aagcggaatt gctctcttat caggacaaca acaacgttct ggatccacag gcacaggcac
     1981 aggcggcgag cacgttagtg aatacgctga tgggccagaa gatccagatg gaagcggacc
     2041 tgcggaactt gctgacgtat ctgcgtgagg acgccccgca agttgtgagt gcgcgtaatg
     2101 cgattcagtc attgcaggca caaattgacg aagaaaaaag caaaatcact gcgccacagg
     2161 gtgacaagct aaaccgtatg gcagtggatt ttgaagaaat caaatcaaaa gtagagttca
     2221 acaccgagct gtacaaactg accctgacct ccattgaaaa gacccgtgta gaagcggctc
     2281 gtaagctcaa ggtgctgtca gtgatcagtt cgccacagtt gccgcaggaa tcgtcttttc
     2341 caaatatccc ttatttgatc gcctgctggt tactggtgtg ctgcctgctg ttcggcaccc
     2401 tgaaactgtt gctggctgtt attgaagatc accgagacta acgctgtcgc tgaatgagtt
     2461 tgtgatgaaa ttatttaaat caattttact gattgccgcc tgtcacgcgg cgcaggccag
     2521 cgcgaccatt gatattaacg ctgacccaaa cctgacagga gccgcgccgc ttaccggtat
     2581 tctgaacgga cagaagtcgg atacgcaaaa catgagcggc ttcgacaata ccccgccgcc
     2641 cgcaccgccg gtggtaatga gccgtatgtt tggtgctcaa cttttcaacg gcaccagcgc
     2701 ggatagcggt gcgacggtag gattcaaccc tgactatatt ctgaatccgg gtgatagcat
     2761 tcaggttcgc ttgtggggtg cgttcacctt tgatggtgcg ttacaggttg atcccaaagg
     2821 taatattttc ctgccgaacg ttggtccggt gaaagttgct ggcgtcagta atagtcagct
     2881 aaatgccttg gtcacatcca aagtgaagga agtataccag tccaacgtca acgtctacgc
     2941 ctccttatta caggcgcagc cagtaaaagt gtacgtgacc ggatttgtgc gtaatcctgg
     3001 tctgtatggc ggtgtgacgt ctgattcgtt actcaattat ctgatcaagg ctggcggcgt
     3061 tgatccagag cgcggaagtt acgttgatat tgtggtcaag cgcggtaacc gcgtgcgctc
     3121 caacgtcaac ctgtacgact tcctgctgaa cggcaaactg ggactttcgc agttcgccga
     3181 tggtgacacc atcatcgtcg ggccacgtca gcatactttc agcgttcagg gcgatgtctt
     3241 taacagctac gactttgagt tccgcgaaag cagcattccc gtaacggaag cgttgagctg
     3301 ggcgcgccct aagcctggcg cgactcacat tacgattatg cgtaaacagg ggctgcaaaa
     3361 acgcagcgaa tactatccga tcagttctgc gccaggccgt atgttgcaaa atggcgatac
     3421 cttaatcgtg agcactgacc gctatgccgg caccattcag gtgcgggttg aaggcgcaca
     3481 ctccggtgaa catgccatgg tattgcctta tggttccact atgcgtgcgg ttctggaaaa
     3541 agtccgcccg aacagcatgt cgcagatgaa cgcagttcag ctttatcgcc catcagtggc
     3601 tcagcgtcag aaagagatgc tgaatctctc gctgcaaaag ctggaggaag cctcactttc
     3661 tgcccagtca tccaccaaag aagaagccag cctgcgaatg caggaagcac aactgatcag
     3721 ccgctttgtg gcgaaagcac gcaccgtggt tccgaaaggt gaagtgatcc tcaacgaatc
     3781 caatattgat tctgttctgc ttgaagatgg cgacgtcatc aatattccgg agaaaacatc
     3841 gctggttatg gttcatggcg aggtgctgtt cccgaacgcg gtgagctggc agaaagggat
     3901 gaccaccgag gattacatcg agaaatgcgg tggcctgacg caaaaatcgg gtaacgccag
     3961 aattatcgtc attcgtcaga acggtgcggc agtcaacgct gaagatgtag attcactcaa
     4021 gcctggcgat gagattatgg ttctgccgaa atatgaatcg aaaaacattg aagttacccg
     4081 tggtatttcc accatcctct atcagctggc ggtgggtgca aaagtgattc tgtctttgta
     4141 aggggttgaa atgagcaaag cagttattgt cattccggct cgctatggct cctcgcgcct
     4201 gccgggtaag ccactgctcg atattgttgg taaaccgatg atccagcatg tttacgagcg
     4261 tgcgttacag gtggcgggcg ttgcggaagt ttgggtggca acagacgatc cgcgtgttga
     4321 acaggccgta caggcgtttg gcgggaaagc catcatgacg cgcaacgatc atgaatccgg
     4381 caccgatcgg ctggtcgagg tgatgcataa agtcgaggca gatatctaca ttaacctgca
     4441 gggcgacgaa ccaatgattc gcccgcggga tgtagaaacg ctgctacaag gaatgcgtga
     4501 cgatcccgcg ttgccggtgg caacgctatg ccacgcgatt tctgccgcag aagcggctga
     4561 gccaagcacg gtaaaagtgg ttgtgaatac ccgccaggat gcgctttatt tcagccgctc
     4621 accgattccg tatccgcgta atgctgaaaa agcgcgctac ctgaaacacg tcggtattta
     4681 cgcttatcgt cgcgatgtgc tgcaaaacta cagccagtta ccggagtcca tgccggagca
     4741 ggcggaatca ctggagcagc taaggttgat gaacgcgggg atcaacatcc gcacatttga
     4801 ggttgccgca accggtccgg gcgtcgacac cccagcatgc ctggaaaaag tgcgcgccct
     4861 gatggcacag gaactggctg aaaacgcatg attggcattt actcgcctgg catctggcgt
     4921 attccgcatc tggagaaatt tctggcgcaa ccgtgccaga aactttctct gctgcgccct
     4981 gttccacaag aagttgatgc tatcgccgtg tggggacatc gtcccagcgc ggcgaaacca
     5041 gtcgccatcg ccaaagcagc gggaaaaccc gtcattcgtc tggaagatgg atttgtgcgt
     5101 tcgctggatc ttggcgtcaa tggcgagccg ccgctttctc tggtggtgga tgattgttgc
     5161 atttactacg atgccagcaa gccttcagca ctggagaaac tggtacaaga taaagccgga
     5221 aatacagctc tgataagcca ggccagagaa gcgatgcaca ccatcgtgac cggggatttg
     5281 tcgaaatata acctggcacc tgcgtttgtg gctgatgagt cagaacgttc agacatcgtt
     5341 ctggttgtcg atcagacatt taatgatatg tcagtgacgt atggcaatgc tggcccgcat
     5401 gagtttgctg ccatgctgga agccgcgatg gcggaaaatc ctcaagccga aatttgggtg
     5461 aaggtgcatc cggatgtcct ggaaggaaag aaaacaggtt atttcgccgc tctgcgcgcc
     5521 acgcaacgag tacgtttaat tgccgagaat gtcagcccgc agtcgctgtt gcgacacgtt
     5581 tcccgggttt acgtcgtgac ctcccagtac ggctttgaag ccttgctggc aggaaaaccg
     5641 gtgacatgct tcggccagcc ctggtatgca ggctggggct taaccgacga tcgccatccg
     5701 cagtccgctt tgttatctgc ccgacgcggt tctgccacgc tggaggaact ttttgccgct
     5761 gcatacctgc gttactgtcg ctatatcgat ccgcaaacgg gagaagtaag caatctattt
     5821 accgtgctgc aatggctgca attacaacgt cgacatctgc aacagcgtaa tggttattta
     5881 tgggcgccag gcttaacgct gtggaagtca gcgatcctga aacctttctt gcaaacggca
     5941 acaaaccggc tgagtttttc acgtcgctgt actgcggcga gcgcctgcgt ggtatggggt
     6001 gtaaagggag aacagcaatg gctagccgaa gcgcagcgaa aatcactgcc gttatggcga
     6061 atggaagatg gttttctacg ttcatccgga cttggctctg accttttgcc gccgctatcg
     6121 ttggtcctgg ataaacgcgg gatctactat gacgccacgc gccccagcga cctggaagtg
     6181 ctgcttaatc acagccagtt aacactggcg cagcagatgc gagctgaaaa attacgccag
     6241 cgactggttg aaagcaaact gagcaaatac aacctggggg ccgatttctc tctgcctgcc
     6301 gaggccaaag ataaaaaaat catcctggtg ccgggtcagg tagaagacga tgcctcaatt
     6361 aaaacaggca ctgtgtcgat taagagcaac cttgagttat tacgcacagt acgcgagcgc
     6421 actccgcacg cttacattat ttataaaccg cacccggatg tactggtggg gaatcgcaag
     6481 ggcaatattc cgacagaact aattgctgaa ctcgctgatt atcaggcact ggacgcagat
     6541 attattcaat gcattcagcg cgcagatgaa gtgcacacca tgacatcatt gtccgggttt
     6601 gaagcgttat tacatggcaa gcaagttcat tgttacggcc tgcccttcta tgccggttgg
     6661 ggtttaaccg ttgatgaaca tcactgcccg cgtcgcgagc aaaaattaac gatagcagat
     6721 ttgatctatc aggcgttgat tgtttatcca acctatatcc acccaacact gctacaacct
     6781 attacggttg aagaggcggc ggaatatttg atccagacgc cgcgcaagcc gatgtttatt
     6841 acccgaaaaa aagcgggacg agtaatacgc tattaccgca aattaattat gttctgcaag
     6901 gtcagatttg gctaaacaat ttcacagttg actattacac aaattattta tgcaaggtaa
     6961 tgcactaacc gttttattat ccggtaaaaa atatctgcta ttgcaggggc caatgggacc
     7021 ctttttcagt gatgttgccg agtggctaga gtcattaggt cgtaacgctg tgaatgttgt
     7081 attcaacggt ggggatcgtt tttactgccg ccatcgacaa tacctagctt actaccagac
     7141 accgaaagag tttcccggat ggttacggga tctccaccgg caatatgact ttgacacaat
     7201 cctctgcttt ggcgactgcc gcccattgca taaagaagca aaacgctggg caaagtcgaa
     7261 agggatccgc ttcctggcat ttgaagaagg atatttacgc ccgcaattta ttaccgttga
     7321 agaaggcgga gtgaacgcat attcatcgct accgcgcgat ccggattttt atcgtaagtt
     7381 accagatatg cctacgccgc acgttgagaa cttaaaacct tcaacgatga aacgtatagg
     7441 ccatgctatg tggtattacc tgatgggctg gcattaccgt catgagtttc ctcgctaccg
     7501 ccaccacaaa tcattttccc cctggtatga agcacgttgc tgggttcgtg catactggcg
     7561 caagcaactt tacaaggtaa cacagcgtaa ggtattaccg aggttaatga acgaactgga
     7621 ccagcgttat tatcttgctg ttttgcaggt gtataacgat agccagattc gtaaccacag
     7681 cagttataac gatgtgcgtg actatattaa tgaagtcatg tactcatttt cgcgtaaagc
     7741 gccgaaagaa agttatttgg tgatcaaaca tcatccgatg gatcgtggtc acagactcta
     7801 tcgaccatta attaaacggt tgagtaagga atatggctta ggtgagcgaa tcctttatgt
     7861 gcacgatctc ccgatgccgg aattattacg ccatgcaaaa gcggtggtga cgattaacag
     7921 tacggcgggg atctctgcgc tgattcataa caaaccactc aaagtgatgg gcaatgccct
     7981 gtacgacatc aagggcttga cgtatcaagg gcatttgcac cagttctggc aggctgattt
     8041 taaaccagat atgaaactgt ttaagaagtt tcgtgggtat ttattggtga agacgcaggt
     8101 taatgcggtt tattattaac atcttatcaa caatcgacat ctccattata t
//
LOCUS       DAZFRG010000208         8121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFRG010000208 DAZFRG010000000
VERSION     DAZFRG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.209"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..988
                     /gene="yjjP"
                     /locus_tag="ACHKUC_003843"
     CDS             218..988
                     /gene="yjjP"
                     /locus_tag="ACHKUC_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO6620020.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            979..1452
                     /gene="yjjB"
                     /locus_tag="ACHKUC_003844"
     CDS             979..1452
                     /gene="yjjB"
                     /locus_tag="ACHKUC_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO6620021.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1560..2099
                     /gene="dnaT"
                     /locus_tag="ACHKUC_003845"
     CDS             1560..2099
                     /gene="dnaT"
                     /locus_tag="ACHKUC_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO6620022.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2102..2839
                     /gene="dnaC"
                     /locus_tag="ACHKUC_003846"
     CDS             2102..2839
                     /gene="dnaC"
                     /locus_tag="ACHKUC_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO6620023.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2888..3382
                     /gene="yjjA"
                     /locus_tag="ACHKUC_003847"
     CDS             2888..3382
                     /gene="yjjA"
                     /locus_tag="ACHKUC_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO6620024.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3636..5927
                     /gene="opgB"
                     /locus_tag="ACHKUC_003848"
     CDS             3636..5927
                     /gene="opgB"
                     /locus_tag="ACHKUC_003848"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO6620025.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6067..7089)
                     /gene="lgoD"
                     /locus_tag="ACHKUC_003849"
     CDS             complement(6067..7089)
                     /gene="lgoD"
                     /locus_tag="ACHKUC_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO6620026.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7228..>8121
                     /locus_tag="ACHKUC_003850"
     CDS             7228..>8121
                     /locus_tag="ACHKUC_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6620027.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQ"
BASE COUNT         2058 a         2098 c         1979 g         1986 t
ORIGIN      
        1 aaggccaaat aacaaattca ttatagtgat aatattcatg cgatagtttg tttacggcga
       61 gagataattc gctttttatc accgagcatc tcccgataac caggattaat cagcgcaatt
      121 ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt ctcattatct
      181 gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc aacagcgagc
      241 cgtaacacgg ctttgtatcc agtgtggatt atttcttttg caacatggtg cggaaagcgc
      301 gttggttgat gagctttcct cacgactggg tcgggcgttg ggaatggaca gcgtcgagag
      361 ttctatctct tcgaacgcca tagtgctgac aaccattaaa gatgggcaat gcctgacatc
      421 gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag tccagcacat
      481 tgtaattctg gcggagcatc atctgctgga ttacaaaggc gtagagaaac gatttagcca
      541 aattcagcca ttacgttacc caagatggct ggtagcctta atggttggcc tttcctgcgc
      601 ctgtttctgt aaactcaata aaggtggctg ggatggtgcc gtcatcacct tcctggccag
      661 tacggccgcg atgtatatcc gccagctgct ggcacaacgt catcttcatc cacagatcaa
      721 cttttgcctc accgctttcg ccgccaccac catttccgga ttgcttttgc aacttcccac
      781 tttcagcaat acccccacca ttgcgatggc cgccagcgtt ctgctgctgg tgccgggctt
      841 tccgttgata aatgccgtcg ccgatatgtt taaaggccac atcaataccg gactggcacg
      901 ctgggcaatc gccagtctgc tgacactggc tacctgtgtc ggcgtagtaa tggcactgac
      961 gatttggggg ctacgcggat gggtgtgatc gaatttctgt tagcgttggc gcaggatatg
     1021 atcctcgccg ccattcctgc ggtcggcttt gcgatggtgt tcaacgttcc cgttcgagcg
     1081 ttacgctggt gtgcgctgct tggtgcgata ggtcatggtt cccgaatgat cttaatgacc
     1141 agcgggctga atattgaatg gtcaaccttt atggcctcta tgctggtcgg caccattggt
     1201 attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc ggctgttatc
     1261 cctatgttcc ctggcatatc agcttatacc gcaatgattt cggcggtaaa aatcagccag
     1321 ctaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac agcttcatcg
     1381 attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta ccgcaagcgc
     1441 cctcgcgtat aaaattgcct cacttaaagc acgcggatga gagacagcct cctctcctcc
     1501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     1561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     1621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     1681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaaactgg
     1741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     1801 ccgttgctgt accaatgggg aaatttgcca tgtatccgga ctggcaaccc gatgccgact
     1861 ttatccgcct ggcggcgcta tggggcgtgg cactaagaga gccggtgacc gccgaagaac
     1921 tggcgtcatt cattgcctac tggcaggcgg aaggcaaagt ctttcaccat gtgcagtggc
     1981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     2041 gagatgtgaa tacggtcagt gaacctgaca gccaaattcc accaggattc agagggtaac
     2101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     2161 acctgcgttc aaaacgggtg aagaattact ggcctggcag aaagaacaag gggcgatccg
     2221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     2281 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     2341 acaaatgaat gcgttaagca aagcgcgcca gtatgtcgag gagtttgacg gcaacatcgc
     2401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     2461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     2521 ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct
     2581 gagtaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     2641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     2701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     2761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgtgt
     2821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttcaaccttg
     2881 agtaatgatg atgaaaacta ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     2941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     3001 acttggcaac caaaacaaca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     3061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     3121 tctgcaatac tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa acatcaagaa
     3181 tcaggtactg gaaaaactgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     3241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataatat
     3301 tggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     3361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     3421 gcggctctct cctgcccttt tccctgctcc tggacggttt tacccctgtt acacgggaag
     3481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     3541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa aacttaaacg
     3601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     3661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     3721 cggccacatt aacggtactg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     3781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     3841 ccggtgctgg tgtcagcaaa tacatcctgc ctggtatcgg cattgtgctg gggctggcgg
     3901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg tcatcatccg caccattttg
     3961 gttacagcct gctggcactc ttactggcgc tgggttcagt ggatgccagc ccggcatttc
     4021 gtcagataac ggaactggtg aaatcccagt cacgcgacgg cgacccggac tttgcggctt
     4081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     4141 gcgaaagtct cgagcggacc tattttgata acgaggcttt cccggatctc acgcctgaac
     4201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     4261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccatttg
     4321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     4381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     4441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     4501 aaagtgtggt ggccgacccg cactatcgta acgactgggg attctacgac gataccgttc
     4561 tcgatgaagc gtggaaaaaa tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     4621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgcacc tgtaaccgca
     4681 aaaaatatga ttttgacggt aagccgaatc agtcattcag cgcggtaagc tgcagccagg
     4741 agaatattgc gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     4801 tcgtcgtctc ttctgaccat ttagcgatga acaatacggc gtggaaatac cttaataagc
     4861 aggatcgcaa taacctgttt tttgtcatcc gtggcgacaa gccgcagcaa gagacgctgg
     4921 cagtgaagcg taatacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     4981 actatctcgg acttggccgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     5041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     5101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcaggtagcc
     5161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     5221 gcgaatactc agcaccgctg cgtttccagc tggcagattt cgctccacgc gacaatttcg
     5281 tctgggttga ccgttgctac aagatggcac aactctgggc tccggaactg gcactctcca
     5341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcag catgttgaca
     5401 aagcaatatg gaagggcaaa acggcattta aagatacggt catcgacatg gcgcgttaca
     5461 aaggcaatgt cgatacgcta aagattgttg ataacgatat tcgctacaaa gccgacagtt
     5521 tcatctttaa cgtcgctggt gcgccagaag aggtgaaaca gtttagcggg atttctcgtc
     5581 cagagtcgtg gggtcgctgg tccaacgcac agctgggtga tgaagtaaaa atcgagtaca
     5641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     5701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     5761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     5821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     5881 tcgggatcgg catggtggaa attaaagtgg tggaacgtga agggtaattc ctgactgatg
     5941 tgtaggtcgc ataaggtgcg acctacacac cttctccggc ctacaaaaca ttattgagaa
     6001 acagaaaaaa aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc
     6061 gccttatcag aaagtaatta cgcccttaat caactcacga ttgttaatca catcgcgctc
     6121 gtaggtttct gccagcgtgg cgaacggata gcggtgggtt aacatcatgt cggcagtgat
     6181 tttcccttcc gccatcaatc gaccgacttt agcgaaatct tccggcgtgg cgttgcggct
     6241 gcccatcatc gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt
     6301 aaacaggccg acaaatacca ccgtgccgcc gtgacgaatc agatttacgg tgttattcat
     6361 cgcatgttga ttacctgtcg cgtcaatcac tttctgcgcc agcgaaccac caaactgcgc
     6421 ccgcagctgc gcgtcaaaat cttcggctga cgggtccaga acaggtaatt ccagacgcgt
     6481 tgccacatgt tcacggcgcg ccggactggt atccgccacc accacctgcg cgccatcggc
     6541 tttagcgatt gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc
     6601 gctgggtgca atagctgcac gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa
     6661 tgctgccgcc tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa
     6721 atactcacta aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt
     6781 ggtacgaccg cttttacacg ccgggcattg ctgacaagaa atatacggaa ttaccgcaac
     6841 ttgctgtccg atattaaaat tgtgaatgtt ttttcctgat tcaataatct ccccacatat
     6901 ttcatggcct aaaacgcgtg gataactaaa aaatggctga ttgcccgccc aggcatgaat
     6961 atctgtaccg caaatcccga cagttttaat tttaattaat gcttcattat cgcctggcac
     7021 cggtctttcc cggacttccc atactaaatt ctttatttcc tgacaaatta atactttcat
     7081 tgtggacatg attcaccgct ccttttattt tatgactttc agatatcgtc gaatttataa
     7141 gagcgcctga atttacccat aaaatatgcg gatgatcgcg cataaaaatg ttttaaatcg
     7201 gcttttaatg ccggactgga gggaagaatg agtcgttcgc agaatttacg tcatagcgtc
     7261 ataaatcagg tgattgaaga tatggcgagg ggcaatattc cttctccgct gccttcacaa
     7321 agcggactgg cagaaatgta taacatcagt cgcactacgg tacgccacat actccagcat
     7381 ttaagcgcat gtggcgtgct caccctggtg gggaaaaatt atgttatcgc ccgcaaaccc
     7441 gaacagaacg atggatttga ttgcatcacg gcctcgctga aagagcaaaa ccgtcttttc
     7501 gagcaggcat ttttcaccat gattaacctg cgccagttac gcgccggaga aagcttttcc
     7561 gaacttcagc tggcgcgagc ggcaagcgtc agtccggtag tggtacgaga atatcttttg
     7621 aaattcggtc gttataatct tattcaaagc gaaaagcgcg gccaatggag catgaagcag
     7681 ttcgatcaat cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattcc
     7741 ctgcaacact ttctcaacct tcccgatgat gaccctcgct ggctgcaagc aaaaaccatg
     7801 ctcgaacgcc accggacgct gcgcgacagc attggcagca actttcgaat gttttctcaa
     7861 ttagaccgtg attttcatgc catgctgtta tccgctgctg acaacatctt ttttaatcaa
     7921 tcacttgaaa ttatatcggt gatctttcat tttcactatc aatgggatga aagcgatctc
     7981 aaacagcgca atatcattgc tgttgatgaa catatgacca ttctcagcgc cctgatttgc
     8041 cgtagcgatt tagatgcaat aacggcgttg cgaaaccatc tggataccgc caaacaatca
     8101 atgattcgtt ccatcaggca a
//
LOCUS       DAZFRG010000209         8112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFRG010000209 DAZFRG010000000
VERSION     DAZFRG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.210"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..223
                     /gene="ypfM"
                     /locus_tag="ACHKUC_003851"
     CDS             164..223
                     /gene="ypfM"
                     /locus_tag="ACHKUC_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO6620028.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(322..3435)
                     /gene="acrD"
                     /locus_tag="ACHKUC_003852"
     CDS             complement(322..3435)
                     /gene="acrD"
                     /locus_tag="ACHKUC_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO6620029.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(3599..5299)
                     /gene="narQ"
                     /locus_tag="ACHKUC_003853"
     CDS             complement(3599..5299)
                     /gene="narQ"
                     /locus_tag="ACHKUC_003853"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO6620030.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            5505..7484
                     /gene="aegA"
                     /locus_tag="ACHKUC_003854"
     CDS             5505..7484
                     /gene="aegA"
                     /locus_tag="ACHKUC_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO6620031.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            7552..>8112
                     /gene="nudK"
                     /locus_tag="ACHKUC_003855"
     CDS             7552..>8112
                     /gene="nudK"
                     /locus_tag="ACHKUC_003855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO6620032.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMS"
BASE COUNT         2004 a         2149 c         2124 g         1835 t
ORIGIN      
        1 atctcgaaat gttgcggtgc atcacataca aatcatcacc gcaggaccat agtatttaca
       61 taaggtttgt cccccggagg tagtcatcac agcaatcgag taaattagct tcaccacggt
      121 atattgtttc cgtagaatac ccataattat aagagaggtt gttatgattg aacgtgaact
      181 ggggaactgg aaagacttta tcgaagttat gcttcgtaag taattcctga gacaaagtac
      241 aaacagcaag aacccgctga gcaggttctt aatcggaaag ttgaaaaggg cgacacattg
      301 gcatgtcgcc ttttttattg cttattccgg gcgcggcttc agcgggaagc ggcggcgcac
      361 cagcacaaag aacagcggca cgaagtaaat agccagaata gtggccgaaa tcatcccgcc
      421 cattacgcca gtacccaccg catgctgacc accggaaccg gcacctgtgc tggttgccat
      481 tggcaatacg ccgaagataa atgccagcga ggtcatcaga atcgggcgta aacgctgacg
      541 gcaggcgtgg agcgtcgctt caaacaggtc gtggcctttt tggttcatct cgttagcaaa
      601 ctcgacgatc aggatagcgt ttttcgccga taaaccaatg accgttaaca gacccacctg
      661 gaagtaaaca tcgttttcca gcccgcgcat ccaggttgcc agcagcgcgc cgataacccc
      721 cagcggcacg accagcatta ccgagaacgg caccgaccag ctttcataca atgccgccag
      781 acacaggaat accaccagca aggaaatggc gtacagcgcc ggagcctgcg cgccggaaag
      841 ccgctcctga tacgacatcg ccgtccactc cagaccaaag ccgtttggca actgcttcac
      901 taacgattcc ataatatcca tcgccgtacc ggtactgacc cccggtgcgg cttccccaac
      961 aatctcaacc gcagaataac cattatagcg ttccagacgc ggcgagcctg tctcccagcg
     1021 tgaggtcgcg aaagcagaga agggcaccat gccgccatct ttatttcgga cataccagag
     1081 attgatgtca tctggcagca tgcgatacgg cgcagctgcc tgcacataga ctttcttcac
     1141 gcgaccgcga tccataaagt cattcacata gctagaaccc caggcggttt gcagtgtgtc
     1201 gttaatatcg tcgatagcaa cgcccagcgc ctgagcttta cgctggtcga tatcaatctg
     1261 caactgcgga ctgtcgtcga ggccgttatg gcgcacacgg gttagctccg ggttttccgc
     1321 tgccagcgcc agcaactggt ttcgtgctgc catcagcgca tcgtgacccg ctccagcgtg
     1381 gtcctgcaac tccatatcaa aacccgcaga actaccaaga ccgctaattg ctggcgggct
     1441 gctggcgata acgcgagctt ctttaatctt gttaaacgcc ttcgttgcac gctcgataat
     1501 ggcaaacgag gtgccggtct tactgtcgcg ttcgctccag tctttcaggc ggataaacat
     1561 tcgcgccacg ttttgcccgt tacccccagg gccagaaccc acggtggcaa acaccgacat
     1621 gatgttgtct ttttcatggg tgaagtaata tttctcgatt tgctcaacga ctttcagggt
     1681 ctgttgctgg gttgaaccgc tgggcaactg taccgaggta gtaaacatgc cacggtcttc
     1741 cagcggtaag aacgacgtcg gcaaacgcag gaacaggaac accatgccgc caagcagcag
     1801 gacataaatc acaatccagc gcaggctacg gtggagaatt ttcgccaccc ctttttcgta
     1861 gcgttcggcg ttgcggttaa acatctggtt aaaccaggca aaaaagcctt tttgcccgtg
     1921 atgctcacct tttttcagtg gcttaagtag cgtggcacac agagcaggag tgaggatcat
     1981 cgctaccagt actgacagca ccatcgccgc aacaatggta atagagaact ggcgatagat
     2041 ggcaccggtg gtgccgccga agaaggccat tggcacaaat accgccgaaa gcaccatcgc
     2101 aatcccgacc agtgccccct ggatctgccc catcgattta cgtgtggctt cgcgaggagt
     2161 gagtccttcc tcactcataa tacgttcgac gttttccacc accacgatgg catcatccac
     2221 cagcagaccg atcgccagca ccatcgcgaa catggttaag gtgttgacac tgtaaccgaa
     2281 ggcgtaaagc acggagaagg ttcccatcaa caccactggc acggcgatag tgggtatcag
     2341 cgtggcgcgg aagttttgca ggaacagata cataacgagg aaaaccaggg cgatagcttc
     2401 cagaagcgtt ttcaccacgt cttcaatgga ggctttaaca aacgaggtgg tttcatacgc
     2461 caccttgtat tccagtccgt gtggaaaata ctgcgccagc tcgtcgagac gattgagcac
     2521 cagctccgct gtcgccattt cgttcgcgcc ggaggccagt ttaaccccca gcccggaggc
     2581 tggcttacca ttgaagcggc taagataatc gtatttctcc gcccccattt cgacggtggc
     2641 gacatcgccc agccttacct ctgagccgtc ctgattcacc cgcaaggtga tatcgcggaa
     2701 ctgttctggt gtttgcagca gtgactgggc gttaatggtg gcgttgagcg cctgcttatc
     2761 gacggaaggc gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg attcaatggc
     2821 atcggtgaca tctttagccg tcatctggaa actgttgagt ttcgccgggt ccagccagat
     2881 acgcatggaa tattgcgaac cataggcatc gatatccccg acgccattca cgcggcttaa
     2941 cgggtcctga atattactgg caacataatc ggcaatatcc tgtttatcca tcgaaccatc
     3001 ggtagagacg aaggcaatgg tcagaatgtt ggtatcgccg gttttacgca ccgtcacgcc
     3061 ctgattttgc accgcctgcg gtaactttcg catggctgat tgcagctggt tttgtacttg
     3121 ctgcacggct tcgtccggat cggtgcctgc tttaaaactt aacgtgacag atgcctgacc
     3181 ggtgccgctg ctctgagatg acatatacat cagattatcg aggccggtca tattttgctc
     3241 gataacctgg gtcacggtgt tttccagcgt ctgggccgat gcgccgggat agttagcggt
     3301 cactcgcaca ttcggtggcg cgagatcggg gtattgttca acgggcaatg aaaaaatcgc
     3361 cagggtacct gtcagacaca acaggattgc cagcacccag gcaaaaatgg ggcgatcaat
     3421 aaagaaattc gccattaaaa gaggacctcg tgtttctgcg tatcgttgta ggcttggctt
     3481 gcttcactgt agcggcaagg tacgagccaa tcgtggagaa aaaaaggagt taatgtaaat
     3541 tatcatgccc ttttatactg aaccttaagt gccactattc tttggtcaat aagaggcatt
     3601 acatcagctg actttcctca ccctccgcag agcgaaagct aatactcact aacgtgccgc
     3661 cgccggaagg ttgtgaaaaa gtcagcgtcc cacccaatct ttccgcgcgt tcgcgcatga
     3721 tattcagacc ataatggcct tcgggttctt tcggttcacc gatgccaatc ccgttgtcgc
     3781 gaatatagac tgtgtgattg ccatccggcg cggtgacgca gctaacggcg atttcgctgg
     3841 cgttggcgtg cttcatcgca ttcagcaccg cttcgcgaat aatttgcaac aaatgcacct
     3901 gcatttgcgc atccagcgcc agggttggca gacggcagtc gagggtcagt ttggcgctgg
     3961 tttgattttg taacgtatcc agcatttccc tcaacgcgga ggggagatcc gcctgctgca
     4021 gcgtcaggcg gaaagtagtc aacagctcgc gtaactgccg ataagcatca ttcaacgcct
     4081 gggaaaaatc ggccatgata ctttgtgcgg tggcgttatc ttccggtatc gaacgcttca
     4141 gtaacgtcaa ctggatacgt aagtaagaaa gtacctgagc cagtgagtcg tgcaattcgc
     4201 gggcgatggt cgcacgttct tccatcaaca ataactgctg aaaatgcttc tgcgcctgat
     4261 taaagtacaa accgcgtccc agcatcgacg aaacgctgtt aagcagcggt tctgatgatg
     4321 aaacgtgact attttgccag tgcagttcgc cgtaaaccgt ctcttgcatt gtcaccggta
     4381 aaatctgcat cggcaattcc gggtttggct gcccttcgct aatccgccag ttgtcaccga
     4441 catttaactc cagatattca gccgcttcat tgtcgcgaac aatctgcaaa atatggcgga
     4501 aacaatgcac atcaatctgg ctggtgttta gcgcctgcga acactgatac aacacctcca
     4561 gacgacgctt ggcctcgtgg agatcgcggg tcttttcttc tactgacgcc tccagcgaac
     4621 ggtacaattt atgcagctcg ctcgacatct ggttaaaggt ttttgcaagc aggccaagct
     4681 cattcggcag gctggtatcc agcggcggcg agtcgaactg cccgtgttca atacgctgac
     4741 tggcggtaac cagctgattc agcggggcaa ccacctgatg gcgtatgcga cgcaaagtaa
     4801 aaaagaccag cgtgaaaata ccgatgccgc cagccaggga aatcgccacc accagcagca
     4861 ttttgcgttc agcgtagtgc tgtaaagcca gtacgaacag gtctatctga ttaacataat
     4921 tattaatatt ggcctgatac cacggcaaat cacccttgct gagccgatta ttcatctcca
     4981 gccagttggc attcagatgc gcatagcggg tttttactgc ctctggcaca taccagacgt
     5041 tgagattggt taataccggt gaatgcagtg cctgctgaaa taactggcga tgtgcattga
     5101 gttgtggact gccactttgc aaatcgtagc ccaggcggta actctgcata cgcagcgatc
     5161 cggcaatatt gatagcctca gcgtcgcgca aactgctcgc cagcgttagc agagcgatac
     5221 ccgtggaaag aatcgacagc agcacaatgt aaaaaaaggc ccgggccaga ctggccgaga
     5281 cgggtcgttt aacaatcaca cgcgtcttct ccacaaaaaa tcattaatgt tccagttcgc
     5341 tgaaacgcaa tttagtcagt gatgggatta tttgatctgc cacagtttat agcgaaatag
     5401 ttacttattc tgggcacatg aacattgccg attcccctgt aagtcgggta ataacaacac
     5461 tcatataaag aataaggttt ttacaaccaa aaaagaaggt cgttatgaat cgttttatta
     5521 tggccaacag tcagcaatgt ctgggttgtc atgcttgtga aatcgcctgt gtcatggctc
     5581 acaatgacga gcaacatgtc ctgagccaac accattttca tccccgaatt acggttatca
     5641 aacatcagca gcaacgtagt gcagtgacct gtcaccattg tgaagatgcg ccctgcgccc
     5701 gtagctgccc taatggcgca atcagccacg ttgatgacag cattcaggtc aatcagcaaa
     5761 agtgtattgg ctgtaaatcc tgcgtggtgg cctgtccatt tggtacgatg caaatcgttc
     5821 tgacacccgt cgcggcagga aaagtaaaag ccacggcgca taaatgcgac ctttgtgcag
     5881 ggcgcgaaaa cggtcctgcc tgtgtcgaaa actgcccggc ggacgcgctg caactggtca
     5941 ctgacgccgc actctccggc atggcgaaat cccgccgctt gcgcaccgca cgtcaggaac
     6001 aacaaccgtg gcatgccagt accgcggcgc aagaaatgcc ggtaatgagt aaagtcgagc
     6061 aaatgcaggc aacgcccgcg cgtggcgagc cagataaact ggcgattgaa gcgcgcaaaa
     6121 ccggttttga tgaaatttat ctgccattcc gtgccgatca ggcacaacgg gaagcctcgc
     6181 gctgccttaa gtgcggcgag cacagcgttt gtgaatggac ctgcccgctg cataaccata
     6241 taccgcagtg gattgaactg gtgaaagccg gaaacatcga cgccgccgtc gagctttctc
     6301 accagaccaa caccctgccg gaaattaccg gacgcgtttg cccgcaagac cgtttgtgtg
     6361 aaggtgcctg tactattcgc gatgagcacg gcgcagtaac tatcggtaac attgaacgct
     6421 acatttcaga tcaggcgttg gcgaaaggtt ggcgtcctga cttaagccat gtcaccaaag
     6481 tggacaagcg ggtggcgatt atcggtgcgg gtccggcagg gctggcctgt gcagatgttc
     6541 tcacccgcaa tggcgtggcg gtgacggtgt acgatcgcca tccagaaatc ggtggcttgc
     6601 tcactttcgg cattccttct ttcaaactgg ataaatccct gctggcacgc cgtcgggaaa
     6661 tcttcagcgc gatggggatt cacttcgaac tcaattgtga agtgggtaaa gatgtctctt
     6721 tgaattcgct tttggaacaa tacgacgcgg tcttcgttgg cgtaggcact taccgttcca
     6781 tgaaagcggg tttacccaat gaagatgcgc cgggcgttta tgacgcgctg ccgttcctca
     6841 tcgccaacac taaacaggtg atgggactcg aagagctacc ggaagagccg tttatcaata
     6901 cggccggact taacgtcgtg gtactgggcg gcggcgacac cgcgatggac tgtgtgcgta
     6961 ctgcgctgcg ccacggcgcg agtaacgtca cctgcgctta tcgtcgtgat gaagctaaca
     7021 tgccaggctc gaagaaagag gtgaagaacg cccgtgaaga gggggccaac ttcgaattta
     7081 acgtccagcc ggtggcgctt gaactgaatg aacaaggtca cgtctgcggg attcgtttcc
     7141 tgcgcacacg tcttggcgag ccggacgccc aggggcgtcg gcgtccagtg ccggtggaag
     7201 gcagtgaatt tgtcatgcca gccgatgcgg tgattatggc gtttggcttc aatccgcacg
     7261 ggatgccgtg gctggagtcg cacggtgtaa cggtagacaa atggggccgc atcatcgcgg
     7321 atgtggaaag ccagtaccgt taccagacca cgaatccgaa aatcttcgcc ggtggtgacg
     7381 ccgtgcgtgg tgcggatctg gtggttaccg caatggcaga aggacgtcat gcggcacagg
     7441 ggatcattga ctggctgggc gtaaaatcag tcaaatctca ctgatagcct gcgcagacaa
     7501 acccgacttc acagcgtaag ataattgttc atttcgcgct gtggagtcgg tatgacgcaa
     7561 caaatcaccc tcgttaaaga caaaattctc tccgataact atttcaccct gcacaacatt
     7621 acttacgatc tcacgcgcaa agatggcgaa gttatccgcc ataaacgtga agtttacgat
     7681 cgcggtaatg gtgcgacgat cctcctgtac aacgcgaaga aaaagaccgt ggttctgatt
     7741 cgccagttcc gcgtcgctac ctgggttaat ggcaatgaaa gcgggcaact gattgaaacc
     7801 tgcgccgggc tgctggataa cgacgaaccg gaagtgtgca ttcgcaaaga agcgattgaa
     7861 gagacgggct atgaagttgg cgaagtccgc aaattatttg aactgtatat gtcgccaggc
     7921 ggtgtgactg agctaatcca cttttttatc gccgaataca gtgacagcca gcgcgctaac
     7981 gccggtggtg gtgtcgaaga tgaagatatt gaagtgcttg agctgccgtt cagccaggcg
     8041 ctggagatga ttaaaaccgg cgagatacgt gacggtaaga cggtgttatt gcttaactat
     8101 ttgcaaatgt ca
//
LOCUS       DAZFRG010000210         7997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFRG010000210 DAZFRG010000000
VERSION     DAZFRG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.211"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..1112
                     /gene="tdcB"
                     /locus_tag="ACHKUC_003856"
     CDS             123..1112
                     /gene="tdcB"
                     /locus_tag="ACHKUC_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO6620033.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            1134..2465
                     /gene="tdcC"
                     /locus_tag="ACHKUC_003857"
     CDS             1134..2465
                     /gene="tdcC"
                     /locus_tag="ACHKUC_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO6620034.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLLPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            2491..3699
                     /gene="tdcD"
                     /locus_tag="ACHKUC_003858"
     CDS             2491..3699
                     /gene="tdcD"
                     /locus_tag="ACHKUC_003858"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO6620035.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVVLIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            3733..6027
                     /gene="tdcE"
                     /locus_tag="ACHKUC_003859"
     CDS             3733..6027
                     /gene="tdcE"
                     /locus_tag="ACHKUC_003859"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO6620036.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            6041..6430
                     /locus_tag="ACHKUC_003860"
     CDS             6041..6430
                     /locus_tag="ACHKUC_003860"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO6620037.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            6502..7866
                     /gene="tdcG"
                     /locus_tag="ACHKUC_003861"
     CDS             6502..7866
                     /gene="tdcG"
                     /locus_tag="ACHKUC_003861"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6620038.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
BASE COUNT         1944 a         1891 c         2098 g         2064 t
ORIGIN      
        1 aggcaattaa ttgaagttga ttagttattt gttttaattt aaacataaat aatccacctg
       61 tctgttttgc aggtgtcggt tacggttacc tacatattta attcaggcta agaggtttta
      121 taatgcatat tacatacgat ctcccggttg ctattgatga catcattgaa gcaaaacaac
      181 gactggctgg gcgaatttat aaaacaggta tgcctcgctc caactatttt agtgaacgtt
      241 gcaaaggtga aatattcctc aaattcgaaa acatgcagcg taccggttca tttaaaattc
      301 gtggcgcatt taataaatta agttcactga ctgatgcgga aaaacgtaaa ggtgtagtgg
      361 cctgctctgc gggcaaccat gcgcaagggg tttcgctctc ctgcgcgatg ctgggtattg
      421 acggtaaagt ggtgatgcca aaaggtgcgc cgaaatccaa agtcgcggca acgtgcgact
      481 actccgcaga agtcgttttg catggtgata acttcaacga cactattgct aaagtgagcg
      541 aaattgtcga aatggaaggc cgtattttta tcccacctta cgatgatccg aaagtgattg
      601 ctggccaggg aacgattggt ctggaaatta tggaagatct ctatgatgtc gataacgtga
      661 ttgtgccaat tggcggcggc ggtttaattg caggtattgc ggtggcaatt aaatctatca
      721 acccgaccat ccgtgttatt ggcgtacagt ctgaaaacgt tcacggcatg gcggcttctt
      781 tccactccgg agaaataact acgcaccgaa ctaccggcac cctggcggat ggttgtgatg
      841 tctcccgccc gggtaattta acttacgaaa tcgttcgtga attagtcgat gacatcgtgc
      901 tggtcagcga agacgaaatc agaaacagta tgattgcctt aattcagcgc aataaagtcg
      961 tcaccgaagg cgcaggcgct ctggcatgtg ctgcattatt aagcggtaaa ttagaccaat
     1021 atattcaaaa cagaaaaacc gtcagtatta tttccggcgg caatatcgat ctttctcgcg
     1081 tctctcaaat caccggtttc gttgacgctt aattaattcg ttgaggatag gatatgagta
     1141 cttcagatag cattgtatcc agccagacaa aacaatcgtc ctggcgtaaa tcagatacca
     1201 catggacgtt aggcttgttt ggtacggcaa tcggcgccgg ggtgctgttc ttccctatcc
     1261 gcgcaggttt tggcggactg atcccgattc ttctgatgtt ggtattagca taccccatcg
     1321 cgttttattg ccaccgggca ctggcgcgtc tgtgtctttc tggctctaac ccttccggca
     1381 acattacgga aacggtggaa gagcattttg gtaaaactgg cggcgtggtt atcacgttcc
     1441 tgtacttctt cgcgatttgc ccactgttgt ggatttatgg cgttactatt accaatacct
     1501 ttatgacgtt ctgggaaaac cagctcggct ttgcaccgct gaatcgcggc tttgtggcgc
     1561 tgttcctgtt gctgctgatg gctttcgtca tctggtttgg taaggatctg atggttaaag
     1621 tgatgagcta tctggtatgg ccgtttatcg ccagcctggt gctgatttct ctttcgctgc
     1681 tcccttactg gaactctgca gttatcgacc aggttgacct cggttcgctg tcgttaactg
     1741 gtcatgacgg tatcctgatc accgtctggc tggggatttc catcatggtt ttctccttta
     1801 acttctcgcc aatcgtctct tccttcgtgg tttctaaacg tgaagagtac gagaaagatt
     1861 ttggtcgcga cttcaccgag cgtaaatgtt cacaaatcat ctctcgcgcc agtatgctga
     1921 tggttgcagt ggtgatgttc tttgccttta gctgcctgtt tacgctgtct ccggcgaata
     1981 tggctgaagc gaaagcgcag aatattccag tgctttctta tctggctaac cactttgcgt
     2041 ccatgaccgg taccaaaaca acgtttgcga ttacactgga atatgccgct tccatcatcg
     2101 cactcgtggc tattttcaaa tctttcttcg gtcactatct ggggacgctg gaagggctga
     2161 atggtttgat tctgaagttc ggttataaag gtgacaaaac caaagtgtcg ctgggtaaac
     2221 tgaatactat cagcatgatt ttcattatgg gctccacctg ggttgttgcc tacgccaacc
     2281 cgaacatcct cgacctgatt gaagccatgg gcgcaccgat tatcgcatca ctgctgtgcc
     2341 tgttgccgat gtatgccatc cgtaaagcgc cgtctctggc gaaataccgt ggtcgtctgg
     2401 ataacgtgtt tgttaccgtg attggtctgc tgaccatcct gaacatcgta tacaaactgt
     2461 tttaatccgt aactcaggat gagaaaagag atgaatgaat ttccggttgt tttggttatt
     2521 aactgtggtt cgtcttcgat taagttttcc gtactcaatg ccagcgactg tgaagtatta
     2581 atgtcaggta ttgccgacgg tattaactcg gaaaatgcat tcttatccgt aaatggggga
     2641 gagccagcac cgctggctca ccacagctac gaaggtgcat tgaaggcaat tgcatttgaa
     2701 ctggaaaaac ggaatttaaa tgacagtgtg gtcttaattg gccaccgcat cgctcacggc
     2761 ggcagtattt ttaccgagtc cgccattatt accgatgaag tcattgataa tatccgtcgc
     2821 gtttctccac tggcacccct gcataattac gccaatttaa gtggtattga atcggcgcag
     2881 caattattcc cgggcgtaac tcaggtggcg gtatttgata ccagtttcca ccagacgatg
     2941 gctccggaag cttatttata cggcctgccg tggaaatatt atgaagagtt aggtgtacgc
     3001 cgttatggtt tccacggcac gtcgcaccgc tatgtttccc agcgcgcgca ttctctgctg
     3061 aatctggcgg aagatgactc cggcctggtt gtggcgcatc ttggcaatgg cgcgtcaatc
     3121 tgcgcggttc gcaacggtca gagtgttgac acttccatgg gaatgacgcc gctggaaggc
     3181 ttgatgatgg gtacccgcag tggtgatgtc gacttcgggg cgatgtcctg ggtcgccagc
     3241 caaaccaacc agagcctggg ggacctggaa cgcgtagtga ataaagagtc gggattatta
     3301 ggtatttccg gcctttcttc ggatttacgt gttctggaaa aagcctggca tgaaggtcac
     3361 gaacgcgcgc aactggcaat taaaaccttt gttcaccgaa ttgcccgtca tattgccgga
     3421 cacgctgctt cattacatcg cctggatgga attatattca ccggcggaat aggagagaat
     3481 tcaagtttaa ttcgtcgtct ggtcatggaa catttagctg tattaggcgt agaaattgat
     3541 acagaaatga ataatcgctc taactctttt ggagagcgaa ttgtttccag tgaaaatgcg
     3601 cgtgtcattt gcgccgtaat tccgactaac gaagaaaaaa tgattgcttt ggatgccatt
     3661 catttaggca aagtgaacgc gcccgcagaa tttgcataat ttagttgaag tattgtagag
     3721 agattatttt tcatgaaggt agatattgat accagcgata agctgtacgc cgacgcatgg
     3781 cttggcttta aaggtacgga ctggaaaaac gaaattaatg tccgcgattt tattcaacat
     3841 aactatacac cttatgaagg cgatgaatct tttctcgccg aagcgacgcc tgccaccacg
     3901 gaattgtggg aaaaagtcat ggaaggcatc cgtatcgaaa atgcgactca cgcgccattg
     3961 gatttcgata ccaatattgc cactacaatt accgctcatg atgcaggata tattgaccag
     4021 ccgctggaaa aaatagtggg tctgcaaacg gatgcgccat taaagcgtgc gctgcatccg
     4081 ttcggcggca ttaatatgat tagaagttca ttccacgcgt atggtcgtga aatggatagc
     4141 gaatttgaat acatctttac cgatctgcgt aaaacccata accagggcgt atttgatgtt
     4201 tactcaccgg atatgctgcg ctgccgtaaa tctggtgtac tgaccggttt accagatggt
     4261 tatggtcgtg ggcgtatcat cggtgactac cgccgcgtgg cgctttacgg catcagttat
     4321 ctggtacgtg aacgcgaact gcagtttgcc gatctccagt cccgtctgga aaaaggcgaa
     4381 gatctcgaag ctacgatccg tctgcgtgag gagctggcgg aacatcgtcg tgcgctgttg
     4441 cagattcagg aaatggcggc gaaatacggt tttgacatct cccgtccggc gcagaatgca
     4501 caggaagcgg tgcagtggct ctacttcgct tacctggcgg cagtgaaatc gcaaaatggc
     4561 ggcgcgatgt cgctggggcg cacggcatcg ttcctcgata tctacattga gcgcgacttt
     4621 aaagcaggcg tactcaatga gcagcaggca caggaactga tcgaccactt catcatgaag
     4681 atccgtatgg tgcgcttctt gcgtacgccg gaatttgatt cgctgttctc cggcgacccg
     4741 atctgggcga cggaagtgat cggcgggatg gggctggacg gtcgaaccct ggtgaccaaa
     4801 aactccttcc gctatttgca caccctgcac actatggggc ctgcaccgga acctaacctg
     4861 actattctgt ggtcggaagc cttgccgatt gccttcaaaa aatatgccgc gcaggtgtct
     4921 atcgtcacat cctctttgca gtatgaaaac gacgatctga tgcgtactga cttcaatagc
     4981 gacgattacg cgattgcctg ttgtgtcagc ccaatggtga ttggtaagca aatgcagttc
     5041 tttggagcac gcgctaacct ggcgaaaacg ctactgtacg caatcaacgg cggaatggac
     5101 gagaagctga agattcaggt tggcccgaaa acggctccgt tgatggatga cgtgctggac
     5161 tacgacaaag tgatggatag cctcgaccac ttcatggact ggctggcggt acagtacatc
     5221 agcgcgctga atatcattca ctacatgcac gacaagtaca gctacgaagc ttcgctgatg
     5281 gcgctacatg atcgtgatgt ctatcgcacc atggcgtgcg gcatcgcggg cctgtcggtg
     5341 gcaacggact ccctgtctgc catcaaatat gctcgtgtga aaccaatccg tgatgaaaac
     5401 ggcctggcgg tggatttcga gattgacggc gaatatccgc agtatggcaa caacgacgag
     5461 cgcgtagaca gcattgcctg cgatctggtt gaacgcttta tgaagaaaat taaagctttg
     5521 ccaacctatc gcaatgctgt gccgacccaa tcgattctga ctatcacctc taacgtggtg
     5581 tacggtcaga aaaccgggaa cacgccggac ggtcgccgcg ccggaacacc gttcgcgccg
     5641 ggcgctaacc cgatgcacgg tcgtgatcgc aaaggcgccg tggcctcatt gacgtcggtg
     5701 gcgaaactgc cgttcaccta cgccaaagat gggatctcgt acaccttctc catcgtccct
     5761 gcggcgctgg gcaaagaaga tccagtacgt aaaaccaacc ttgtcggcct gctggatggg
     5821 tatttccacc acgaagcgga tgtcgaaggc ggtcaacacc tcaacgtcaa cgtaatgaat
     5881 cgggaaatgc tgctggatgc catcgagcac ccggaaaaat atcctaacct gacaatccgt
     5941 gtctctggct acgccgtgcg ctttaacgca ctgacccgtg aacagcaaca ggatgttatt
     6001 tcacgtacct ttacccaggc gctctgacgc cggaggatgt atgaaaaaga ttatcgaaac
     6061 gcaacgtgcc ccaggcgcaa tcggccctta tgttcagggc gttgatttag gcagcatggt
     6121 cttcacctcc gggcaaattc cggtttgccc acagaccggt gagatcccgg ctgatgtgca
     6181 agatcaggcg cgtttaagcc tcgaaaacgt caaagcgatc gtggttgctg ccgggctgag
     6241 tgtgggcgat atcatcaaga tgaccgtgtt catcaccgat ctgaatgatt ttgccaccat
     6301 caatgaagtc tataagcagt tcttcgatga acatcaggcg acctacccga cccggagctg
     6361 tgtgcaggtc gcgcgtttgc cgaaagatgt gaagttggaa attgaagcca tcgcagtacg
     6421 tagtgcgtaa ggcctcgtga gcgggacggt cgcaaggtcg ttccgctcca ctttactgaa
     6481 cggcaatccg agggtgtgga tatgattagt gcattcgata ttttcaaaat tgggattggt
     6541 ccctccagtt cgcatactgt ggggccaatg aatgccggaa aaagttttat tgatcggctg
     6601 gaaagtagcg gcttattaac cgcgacgagc catattgtgg tcgatctgta cgggtcgttg
     6661 tcactgacgg gcaaaggcca tgccacggat gtcgccatca tcatgggact ggcaggaaac
     6721 agtccgcagg atgttgtcat tgatgagatc cctgcattta tagagttagt gacgcgcagc
     6781 gggcggttgc cggtggcttc tggcgcgcat attgttgatt ttcctgtagc aaagaacatt
     6841 atcttccatc ccgaaatgtt gcctcgccat gagaacggaa tgcggatcac tgcctggaag
     6901 ggacaggaag agctattaag caaaacctat tactctgtcg gcggcgggtt tattgtcgaa
     6961 gaagaacact tcggcctgtc gcacgatgtc gaaacacccg taccttacga tttccactca
     7021 gcaggtgaac tgctgaaaat gtgtgattac aacggcctgt ctatatctgg cctgatgatg
     7081 cacaacgagc tggcgctgcg cagtaaagcg gaaattgacg cagggtttgc ccgtatctgg
     7141 caagtgatgc atgacggtat tgaacgtgga atgaatactg aaggcgtgct gcctggtccg
     7201 ctcaatgtgc cgcgccgtgc cgtagcgctg cgtcgtcaac tggtttccag cgataacatc
     7261 tctaacgatc cgatgaatgt catcgactgg atcaacatgt acgcgctggc ggttagtgaa
     7321 gaaaacgcag ctggcgggcg cgtggtaacg gcaccgacta acggtgcgtg cggcattatt
     7381 ccggcagtgc tggcttatta cgataagttc cgtcgtccgg taaacgagcg gtcaattgcc
     7441 cgctattttc tggcagcggg ggcaattggc gcgctgtata aaatgaacgc ctccatctct
     7501 ggcgcggaag tcggctgtca gggggagatt ggtgtagcct gttcaatggc ggcggcaggg
     7561 ttaactgaac tactgggcgg cagtccggcg caggtatgca atgcggcgga aatcgcgatg
     7621 gagcataacc ttgggctgac ctgcgatccg gttgccggac aggtacaaat cccgtgcatt
     7681 gaacgtaatg ccattaatgc cgtgaaagca gtaaacgccg cgcggatggc aatgcgccgc
     7741 acctcggcac cgcgtgtttc actcgataaa gtgatcgaga cgatgtatga aaccggcaaa
     7801 gatatgaacg ataaataccg cgaaacatca cgcggaggac tggctattaa agtggtctgc
     7861 ggctgacatt gctgtcagct atcatccttg ggtgcacaaa atgtgcaccc ttttttattg
     7921 tgatctgccg caaatagaga ataattattt ctattaactc aatttaatag aaaattatta
     7981 cccacaattg ctattta
//
LOCUS       DAZFRG010000211         7958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFRG010000211 DAZFRG010000000
VERSION     DAZFRG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.212"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..242
                     /gene="ymiA"
                     /locus_tag="ACHKUC_003862"
     CDS             102..242
                     /gene="ymiA"
                     /locus_tag="ACHKUC_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO6620039.1"
                     /translation="MRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVVVALLIWKL
                     WG"
     gene            245..412
                     /gene="yciX"
                     /locus_tag="ACHKUC_003863"
     CDS             245..412
                     /gene="yciX"
                     /locus_tag="ACHKUC_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO6620040.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            526..621
                     /gene="ymiC"
                     /locus_tag="ACHKUC_003864"
     CDS             526..621
                     /gene="ymiC"
                     /locus_tag="ACHKUC_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO6620041.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFCAELLWIITH"
     gene            771..3446
                     /gene="acnA"
                     /locus_tag="ACHKUC_003865"
     CDS             771..3446
                     /gene="acnA"
                     /locus_tag="ACHKUC_003865"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO6620042.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNSGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTC
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELAYYQNDGILHYVIRNMLK"
     gene            complement(3510..4100)
                     /gene="ribA"
                     /locus_tag="ACHKUC_003866"
     CDS             complement(3510..4100)
                     /gene="ribA"
                     /locus_tag="ACHKUC_003866"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO6620043.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4270..5034
                     /gene="pgpB"
                     /locus_tag="ACHKUC_003867"
     CDS             4270..5034
                     /gene="pgpB"
                     /locus_tag="ACHKUC_003867"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO6620044.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWSRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5183..5491
                     /gene="lapA"
                     /locus_tag="ACHKUC_003868"
     CDS             5183..5491
                     /gene="lapA"
                     /locus_tag="ACHKUC_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO6620045.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            5498..6667
                     /gene="lapB"
                     /locus_tag="ACHKUC_003869"
     CDS             5498..6667
                     /gene="lapB"
                     /locus_tag="ACHKUC_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO6620046.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6859..7596
                     /gene="pyrF"
                     /locus_tag="ACHKUC_003870"
     CDS             6859..7596
                     /gene="pyrF"
                     /locus_tag="ACHKUC_003870"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO6620047.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7596..7922
                     /gene="yciH"
                     /locus_tag="ACHKUC_003871"
     CDS             7596..7922
                     /gene="yciH"
                     /locus_tag="ACHKUC_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO6620048.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVALDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1938 a         1861 c         2135 g         2024 t
ORIGIN      
        1 aattcgctgg atttttgact aaagtttctt taggatttgt ctcatcgtat gattaattac
       61 accaatcgtt caacgtcttg ctgataagtg atggcgatta tatgaggtta gcaatgcctt
      121 ctggaaatca ggaaccccgc cgcaatcctg aattaaaacg taaagcctgg ctggcggttt
      181 ttcttggttc tgcacttttc tgggtggttg tcgcactgct tatttggaaa ttgtggggat
      241 aactatggtc ggtcaggagc aactggagtc gtcaccatta tgtcagcata gtgacaatga
      301 accagaagca aaacgggaat gttccgtcgt tattccagac gactggcaac taacatcgca
      361 gcaacaagcc tttatagaac tgtttgctga agatgatcag ccgaaacaat aattatcatc
      421 attcttatta cccattttta atgaattaca gggcttttaa tacaccgcag caataacagg
      481 ttgagttatc tcaacacaaa gtaataaccg ttaagggtgt agcctatgat caacacaaat
      541 atgaaatatt ggtcctggat gggcgcattt tctctgtcga tgctcttctg tgccgaactc
      601 ctctggatca ttactcactg atccctgacc ccgctgtttg ctttgcctca aggtttctcc
      661 tcttttatca atttgggttg ttatcaaatc gttacgcgat gtttgtgtta tctttaatat
      721 tcaccctgaa gagaatcagg gcttcgcaac cctgtcatta aggaggagct atgtcgtcaa
      781 ccctacgaga agccagtaag gacacgttgc aggccaaaga taaaacttac cactactaca
      841 gcttgccgct tgctgccaaa tcactgggcg atatcacccg tctacccaag tcactcaaag
      901 ttttgctcga aaacctgctg cgttggcagg atggtaaatc ggttaccgaa gaggatatcc
      961 acgcgctggc gggatggctg aaaaatgccc atgctgaccg tgaaattgcc taccgcccgg
     1021 caagagtgct gatgcaggac tttaccggcg tacctgccgt tgttgatctg gcggcaatgc
     1081 gcgaagcggt taaacgcctc ggcggcgata ctgcaaaggt taacccactc tcaccggtcg
     1141 acctggtcat tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag gcatttgaag
     1201 aaaacgtacg cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg aaatggggaa
     1261 agcaggcgtt cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta
     1321 acctcgaata tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa tggattgctt
     1381 atccggatac actcgttggt actgactcgc acaccaccat gatcaacggc cttggcgtgc
     1441 tggggtgggg cgttggtggc atcgaagcag aagccgcaat gttaggccag ccggtttcca
     1501 tgcttatccc ggacgtagtg ggctttaaac ttaccggaaa attacgcgaa ggtattaccg
     1561 ccacagacct ggttctcact gttacccaaa tgttgcgcaa acatggcgtg gtggggaaat
     1621 tcgtcgaatt ttatggtgat gggctggatt cactaccgct ggcggatcgc gccactattg
     1681 ccaatatgtc accagaatat ggtgccacct gtggcttctt cccaatcgat gctgtcaccc
     1741 tcgattacat gcgtttaagc gggcgcagtg aagatcaggt cgagttggtc gaaaaatatg
     1801 ccaaagcgca gggcatgtgg cgtaattcgg gcgatgaacc aatttttacc agtgtgttag
     1861 aactggatat gaatgacgtt gaagcgagcc ttgcagggcc aaaacgcccg caggatcgcg
     1921 ttgcactgcc cgatgtacca aaagcatttg ctgccagtag cgaactggaa gtgaatgcca
     1981 cgcataaaga tcgcctgccg gtcgattatg ttatgaacgg acatcagtat cagttacctg
     2041 atggcgctgt ggtcattgct gcgataacgt cgtgcaccaa cacctctaac ccaagtgtgc
     2101 tgatggccgc aggcttgctg gcgaaaaaag ccgtaactct gggcctcaag cggcaaccgt
     2161 gggtcaaagc gtcgctggca ccgggttcga aagtcgtttc tgattatctg gcaaaagcga
     2221 aactgacacc gtatctcgat gaactgggat ttaaccttgt gggatacggt tgtaccacct
     2281 gtattggtaa ctctggaccg ttgcccgatc ctatcgaaac ggcaatcaaa aaaggcgatt
     2341 taaccgtcgg tgcagtgctg tccggcaacc gtaactttga aggccgtatc catccgctgg
     2401 ttaaaaccaa ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg gcgggaaata
     2461 tgaatatcaa cctggcttct gagcctatcg gccatgatcg caaaggcgag ccggtatatc
     2521 tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc ggtagaccaa gtctccacag
     2581 aaatgttccg caaagagtac gcagaagttt ttgaaggcac agctgagtgg aaggaaatta
     2641 acgtcacacg atccgatacc tacggttggc aggaggattc aacctatatt cgcttatcgc
     2701 ctttctttga tgaaatgcag gcaacaccag cacctgtgga agatattcac ggtgcgcgga
     2761 tcctcgcaat gctgggggat tcagtcacca ctgaccatat ctctccggcg ggcagtatta
     2821 agcccgacag cccggcgggt cgatatctac aaggtcgggg tgttgagcgg aaagacttta
     2881 actcctatgg ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc ttcgccaata
     2941 ttcgcatccg taatgaaatg gtgcctggcg ttgaaggggg gatgacgtgc catttacctg
     3001 acagcgatgt cgtctctatt tatgatgccg caatgcgcta taagcaggag caaacgccgc
     3061 tggcggtgat tgccgggaaa gagtatggat caggctccag ccgtgactgg gcggcaaaag
     3121 gtccgcgtct gcttggtatt cgtgttgtga ttgccgaatc gtttgaacga attcaccgtt
     3181 cgaatttaat tggcatgggc atcctgccgc tggaatttcc acaaggtgtg acgcgtaaaa
     3241 cgttagagct taccggggaa gagaagattg atattggtga tctgcaaaat ctacaacccg
     3301 gcgcgacggt tccggtgacg cttacgcgcg cggatggtag ccaggaagtc gtaccctgcc
     3361 gttgtcgtat cgacaccgcg acggagttgg cctactacca gaacgacggc attttgcatt
     3421 atgtcattcg taatatgttg aagtaacgag tatttgcttg ccggttattt tgcttccggc
     3481 aagcaaatta attacacaat gcaagagggt tatttgttca gcaaatgacc cattttctcg
     3541 gctttggtat cgagataatg ttcgttattg gggttacgac ctacaatcaa cggtacgcgt
     3601 tcaataatat taatccctgc ttcggtcaga atttcgactt ttttcgggtt attggttaac
     3661 aagcggactt cattgacgcc aaggagtttg aacatatcag cgcaaagagt gaagtcgcgc
     3721 tcatcagcgg cgaagcctaa ctggtggtta gcctctacag tatcgtaacc ttgatcctgc
     3781 agtgcgtaag cgcggatttt attcagcaga ccaatgttac gaccttcctg acggtgatac
     3841 agcaaaatac cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag ctggaagcca
     3901 caatcgcagc gcaagctgaa cagggcgtca ccggtcagac attcggaatg gacgcgcgca
     3961 agtaccgggg tatgcccgga aatatcgcca tagactagcg cgacatgatc gtgtccggtt
     4021 gccagttctt caaatcccac catcaggaaa tcgccccatg gggttggcag tttggcttct
     4081 gccacacgtt taagctgcat gaaattctcc agataatgct ggttctgtat tggcttattt
     4141 tgccataacg agaaggggtt cacctaatca cgacgcgtcg atcgttcacg gaatggcaca
     4201 aatctgtcaa tttttcctgg aactggcatt ttcagttatg attgtgggac ttatcaaaaa
     4261 ggagaggcca tgcgttcgat tgccagacgt accgcagtgg gagctgcact attgcttgtc
     4321 atgccagtag ccgtatggct ttctggctgg cgttggcaac ctggagaaca aagttggtta
     4381 ctaaaagcgg ctttttgggt tactgaaact gtcacccagc cctggggcgt cattacacat
     4441 ttgattttat tcggctggtt tctctggtgt ctgcgttttc gcattaaggc tgccattatg
     4501 ttatttgcca ttctggcggc ggcaatcctt atgggacaag gcgttaaatc ctggatcaaa
     4561 gacaaagtcc aggaaccacg accttttgtt atctggctgg aaaaaacaca tcatattccg
     4621 gttgatgagt tctacacttt aaagcgagca gaacgcggaa atctggtgaa agaacagttg
     4681 gctgaagaga aaaatatccc acaatatttg cgttcacact ggcagaaaga gacggggttt
     4741 gcctttcctt ccggtcacac gatgtttgct gccagttggg cactgctggc cgttggtttg
     4801 ctgtggccgc gtcggcgaac gttaaccatt gctatcttgt tggtctgggc aacgggagtc
     4861 atgggaagcc gcctgctgct cgggatgcat tggtcacgcg atctggttgt agctacgttg
     4921 atttcgtggg cgctggtggc ggtggcaacg tggcttgcgc aacgaatttg tgggccatta
     4981 acaccacctg cggaagaaaa tcgcgaaatt gcacaacgag aacaagaaag ttaacgctgg
     5041 ttgattttcc gaatttagcc cttaaatcat caacaatgcg cgtggatgcc atttcgcaga
     5101 cggcgcgaaa atggtacttt aaagggctat tgcggtaagt tgaccataat ttattcgccc
     5161 taaccacata acgggaagta atgtgaaata tttactcatt ttcttactgg tgttagcgat
     5221 cttcgtgatt tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt ttaattatct
     5281 gttagcgcaa ggggagtacc gtatttccac attgctggcg gtattgtttg ctgcggggtt
     5341 tgctatcggt tggttgattt gtggcctgtt ctggctgcga gttcgtgttt ccctggcgcg
     5401 cgctgaacgt aaaataaagc gactggaaaa ccagctttca cctgcgactg acgtggctgt
     5461 agcgccgcac tcgtctgcgt cgaaggaata actttctatg ctggagttgt tgtttctgct
     5521 tttgcctgta gccgctgcct atggctggta tatgggccgc agaagtgcgc aacaaaacaa
     5581 gcaagatgaa gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact tcctgcttag
     5641 taatcaacag gataaagcgg tagacctgtt tctcgatatg cttaaagagg atacaggtac
     5701 cgttgaagcc caccttacgc tcggaaacct gttccgttcg cgtggcgaag ttgatcgcgc
     5761 tattcgcatc catcagaccc taatggaaag cgcctcgctg acctatgaac agcggctttt
     5821 ggcgattcaa caactggggc gtgattacat ggctgccggg ttatacgacc gcgcggaaga
     5881 catgttcaat cagctgaccg atgaaactga cttccgcatt ggcgcgctgc aacaactgct
     5941 acaaatttac caggctacca gcgagtggca gaaagcaatt gatgttgccg aacgcctggt
     6001 gaagctgggt aaagataaac agcgcgtcga aattgcccat ttctactgtg agttagctct
     6061 gcagcatatg gccagcgacg atctcgatcg tgccatgact ttgctaaaaa aaggtgcggc
     6121 ggcagataaa aacagcgccc gcgtatccat catgatggga cgcgtgttta tggcgaaagg
     6181 agaatacgcc aaagccgtcg aaagtttgca acgtgtcata tcccaggaca gagaactggt
     6241 cagcgaaacg ctggaaatgc tgcaaacatg ctatcagcag ttgggtaaaa ctgccgaatg
     6301 ggcagaattc ctgcagcgcg cggtggaaga gaacaccggt gccgatgctg aattgatgct
     6361 tgcggatatc atcgaagcgc gcgacggtag tgaggccgca caggtctata ttacgcgcca
     6421 gcttcagcgt catccgacca tgcgtgtgtt ccataagtta atggattacc acttaaatga
     6481 agcagaagaa gggcgtgcca aagagagtct gatggtgctg cgtgacatgg ttggcgagaa
     6541 ggtgcgtagt aagcctcgtt atcgctgcca gaaatgtggt tttaccgcat acactctcta
     6601 ctggcattgt ccgtcttgtc gggcctggtc aaccattaaa ccgattcgcg gtcttgatgg
     6661 cctgtaattt ttaaaaaaat ccgactttag ttacaacata ctaattattg atgttctatt
     6721 gtgctccggc accgacggcg cggaaaagcc tgccagagga gaaatcgcaa ctgttaattt
     6781 tttatttcca cgggtagaat gctcgccgtt tacctgtttc gcgccacttc cggtgcccat
     6841 catcaagaag gtctggtcat gacgttaact gcttcatctt cttcccgcgc tgttacgaat
     6901 tctcctgtag ttgttgccct tgattatcat aatcgcgatg ccgcgatggc ctttgtcgac
     6961 aagatcgacc cacgcgattg tcgtctgaag gtcggcaaag agatgtttac attgtttggg
     7021 ccacagtttg tgagcgaact tcaacagcgt ggttttgata tctttcttga cctgaaattc
     7081 cacgatattc ccaacactgc agcgcacgct gtcgctgctg cggctgactt aggcgtgtgg
     7141 atggtgaatg ttcatgcctc cggtggggcg cgtatgatgg ccgcagcgcg tgaggcactg
     7201 gttccgtttg gcaaagatgc accgcttttg attgctgtga cagtgttgac cagcatggaa
     7261 gccagcgacc tggccgatct tggcgtgaca ctgtcacctg cagattatgc agaacgtctg
     7321 gcggcactga cgcaaaaatg tggccttgat ggtgtggtgt gttctgctca ggaagctgtg
     7381 cgctttaaac aggtattcgg tcaggagttc aaactggtta cgccgggcat tcgtccgcag
     7441 gggagtgatg ctggtgacca gcgccgcatt atgacgccag aacaggcgtt ggcggctggt
     7501 gttgattata tggtgattgg tcgcccggta acgcaatcgg tagatccagc gcagacgctg
     7561 aaagcgatca acgcctcttt acagcggagt gcatgatgag tgattccaac agccgtctgg
     7621 tctactcaac ggaaaccgga cgtattgatg agccaaaagc ggcccctgta cgccctaaag
     7681 gcgacggtgt ggtgcgtatt cagcgtcaga ccagtggacg taaaggtaag ggcgtttgcc
     7741 tgattaccgg tgtcgctctc gatgatgccg aactgacgaa actggcagcg gaactgaaga
     7801 aaaaatgcgg ctgcggcgga gcagttaaag atggaattat tgaaatccag ggcgataagc
     7861 gtgatttatt aaaatcactg cttgaagcga aagggatgaa ggtaaaactc gcaggcggct
     7921 aacataaaaa gccacggata tatccgtggc tttcgaat
//
LOCUS       DAZFRG010000212         7950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFRG010000212 DAZFRG010000000
VERSION     DAZFRG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.213"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1129
                     /locus_tag="ACHKUC_003872"
     CDS             <1..1129
                     /locus_tag="ACHKUC_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO6620049.1"
                     /translation="REIRHQIHGNPELGLQEFKTSALVAEKLRQWGYEVEQGLATTGV
                     VATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGHDGHTTILLGAARY
                     FAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGMHNMPGLPVGKFFF
                     QPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQSIVSRNVDPLEAAV
                     ITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQAQAASFGATAEVT
                     HVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFSFMLEAQPKGGFLL
                     FGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1172..2488
                     /locus_tag="ACHKUC_003873"
     CDS             1172..2488
                     /locus_tag="ACHKUC_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO6620050.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2592..3242
                     /gene="fabR"
                     /locus_tag="ACHKUC_003874"
     CDS             2592..3242
                     /gene="fabR"
                     /locus_tag="ACHKUC_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO6620051.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTTIIPGNVKDE"
     gene            3242..3601
                     /gene="yijD"
                     /locus_tag="ACHKUC_003875"
     CDS             3242..3601
                     /gene="yijD"
                     /locus_tag="ACHKUC_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO6620052.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3641..4741)
                     /gene="trmA"
                     /locus_tag="ACHKUC_003876"
     CDS             complement(3641..4741)
                     /gene="trmA"
                     /locus_tag="ACHKUC_003876"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO6620053.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4855..5045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5110..6954
                     /gene="btuB"
                     /locus_tag="ACHKUC_003877"
     CDS             5110..6954
                     /gene="btuB"
                     /locus_tag="ACHKUC_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO6620054.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLISSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGTYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6899..7756
                     /gene="murI"
                     /locus_tag="ACHKUC_003878"
     CDS             6899..7756
                     /gene="murI"
                     /locus_tag="ACHKUC_003878"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO6620055.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1805 a         1885 c         2168 g         2092 t
ORIGIN      
        1 tcgcgagatc cgccatcaga tccacggaaa cccggagctg ggattacaag aatttaaaac
       61 cagtgctttg gttgcggaaa agctgcgcca gtggggctat gaggttgagc aagggctggc
      121 gacaaccggc gtggtagcca cgttaaaagt gggcgacgga gagaaaagta ttggcctgcg
      181 ggcggatatg gatgcgttgc cgatttatga aaatagtggc aagccctggg ccagtaaaca
      241 tcctggttta atgcacgcct gcgggcatga cgggcatacc accatactgc tgggggctgc
      301 gcgttatttt gccgaaaccc gccgttttaa cggcacgtta cgccttatct tccagcctgc
      361 ggaagagatg attaacggcg gtgagatcat ggttaaagag gggctttttg atcgtttccc
      421 ctgcgatgtc attttcggga tgcacaacat gccgggtttg ccggtcggca agtttttctt
      481 ccagcctggg gcgctgatgg cgtcaatgga tcagttccat attacggttc gtggttgtgg
      541 cggacacggt gcgatcccgc acaaagccat cgatccagtg ctggttgctg cacatatcac
      601 caccgcatta caaagcattg tgtcgcgcaa tgtcgatccg ctggaagcgg cggtaattac
      661 cgtcggcagc attgttgcgg gtgaggcggc taacgtcatt cctgacagtg ctgagatgaa
      721 aatcagtgtc cgctctctta gccgcgatac ccgacaactt cttctgaccc gtattcctgc
      781 tcttgcacaa gcccaggctg ccagctttgg cgctacggct gaggttacgc atgttaacgg
      841 tacaccggta ttagtaaatg acgaggagat ggcgcgcttc gcctggcagg tggcgtgcaa
      901 aacatttggt gaagatcggg ctgagtttgg catcaaaccc ctgatgggca gtgaagattt
      961 ctctttcatg ctggaagccc aacccaaagg cggtttcctg ttattcggta atggcgatgt
     1021 cggggaaggt tcctgcatgg tgcataaccc cggttacgac tttaacgatg caagcctggt
     1081 cccggcaagc agctactggg gcgcactggt tgaagcctgg ctgcaataaa taaaacaaca
     1141 cacaacaaca ctttttggca aggaacatgg aatgacggcg atcgtaaatg aaaccgtgag
     1201 tgtgaagaaa aagcccagcg gcaggcgggt gatctttgcc tcggcgtttg gcaatgcgct
     1261 cgaatttttt gattttggcg tttacaactt ttttgtggtc tatatcagta cgcttttctt
     1321 cccacccagt gccgatcata acgtcgcgct gttactggca tttgcaacgt ttggtgtgag
     1381 cttttttatg cgtccgctcg gtgggattat cgttggtgcc tgggccgatc gcttcgggcg
     1441 taaaccggcg atggtgttca ctattgctct gatgagcctc ggtacgctga tgatcggcat
     1501 cgcaccgacg tatgaaacgg cgggctattg gggaactgca acgctggtgc tggcgcgtct
     1561 aattcagggc gttgcggcag gtggtgaagt cggggcttca atgtcgctac tggtggagtc
     1621 agctccggca aatcgtcgcg gcttttacag cagttggtca ctggcgaccc aagggctggc
     1681 aacgaccttc ggtggggttg ttgctttagg tctgagcgca tggctaccgt tcgccacagg
     1741 ttcagaaacc gtaatggcag aatggggctg gcgtgtgccg ttttttattg gcgtgttatt
     1801 agcacctatc ggttgctggt tgcgtctgag cctggaaaat gacgtacctg agccagtgcg
     1861 caataaaaaa gctgctacca gcgaaagcgc gttttctctg ctcctgcaac ataaggcgac
     1921 tatcgttaat ggcgttttac tcgctattgg tagcacggtc gcaacctata tctccctctt
     1981 ttattacggc acctgggcgg cgaagtatct ggcgatgcct caacattatt ctcatgcagc
     2041 gatgttactg gcgggggtaa ttacgtttgt gggtgcgctg ttggtgggga tgctatgtga
     2101 ttcggttggg cgtaaaaagc tgattttaat ctcccgtgtg atggtgcttc tctgtagctg
     2161 gccgtcattc tggctgttgg tgaattaccc aagccctggc atgttgctga cggtggtttt
     2221 cgtgatggtc agctttacca cgcttggcgg tgtgccagtg atgttgttga tctctgaact
     2281 gttgccgaaa cggattcggg cattaggttt cgcgctggtt tatagcattg gcgtagcaat
     2341 ctttggcggt tttgcgcaat atttcgccac gcaatccatc gtgttgctgg atagtctgac
     2401 ggctccggcg tggtatctgg ggggaggcac gttgctgtcg atgctggcgt tgttgtacgt
     2461 aaaagaaccg gccaaagaat tgcagtaaat atgttttatt gcgttaccgt tcattcacaa
     2521 cactggagca atccagtatg ttcattctct ggtatagtgc cagcagtact tttggcaagg
     2581 attcagacat cgtgatgggc gtaagagcgc aacaaaaaga aaaaacccgc cgttcgctgg
     2641 tggaagccgc atttagccaa ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg
     2701 aagtggcacg agaagcaggc attgctccca cctcttttta tcggcatttc cgcgacgtag
     2761 acgaactggg tctgaccatg gttgatgaga gcggtttaat gctacgccaa ctcatgcgtc
     2821 aggcgcgtca gcgtatcgcc aaaggcggga gtgtgatccg cacctcggtc tccacattta
     2881 tggagttcat cggtaataat cctaacgcct tccggttatt attgcgggaa cgctccggca
     2941 cctccgctgc gtttcgtgcc gccgttgcgc gtgaaattca gcacttcatt gcggaacttg
     3001 cggactatct ggaactcgaa aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag
     3061 caatggtgac gattgtcttc agtgcggggg ccgaggcgtt ggatgtcggc gtcgagcaac
     3121 gccggcaatt agaagagcga ctggtactgc agctgcgaat gatttcgaaa ggggcttatt
     3181 actggtatcg ccgtgaacaa gagaaaacca caattattcc gggaaatgtg aaggacgagt
     3241 aatgaaacaa gcaaatcaag atagaggtac gctgctgcta gcgttggttg ctggcttatc
     3301 gatcaatggt acttttgcag cgctgtttag ctccattgta ccattttctg tattcccgat
     3361 tatttccctg gtgttgacag tttactgcct gcatcaacgt tatcttaatc gcactatgcc
     3421 ggtaggcttg ccgggtctgg cagctgcttg ttttattctc ggcgtactgc tgtacagcac
     3481 ggtagttcgt gcggaatatc cggatatcgg ctctaacttc ttcccggcag tactgtccgt
     3541 cattatggtg ttctggattg gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta
     3601 atcggtatca tgccggatgc aagcgcatcc ggcatgggtt ttacttcgcg gtcagtaata
     3661 cgccgcactc catatggtgc gtgtagggga actgatcaaa tagagccaga cgttcgacct
     3721 tgtgcgtctg gcttaatgtt tccagattct tgcataacgt ttccggattg caggagatat
     3781 acagaatacg tggatacgcc tgcaccattt tctcggtttc actgtccaga ccgctgcgcg
     3841 gtgggtcgac aaaaatggtt tcgcactgat aactctttaa gtcgattcct tgcaggcggt
     3901 taaactcgcg cacaccattc atcgcctgag taaattcttc tgccgccata cgaataattt
     3961 gtacgttatc aatatggtta gcggcaatgt tgtattgcgc ggcggcgact gacggcttgg
     4021 cgatttcggt ggctaatacc cgatcaaaat tgcgtgccag cgctaatgaa aagttaccgt
     4081 tgccgcagta tagctccagt aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt
     4141 ccagcatctg aatattcatc gccgcgttcg gttgggtaaa gctgttttct acctgacggt
     4201 agatcatctc tttccccgcg atcggcagac gttcgtcgat gtaatcctga tccagcgcga
     4261 ttttggtttt cgttgcccga ccaatcagat gcacattcag attctgcgcg cgcagtgcat
     4321 cgcgcagggc ttccgcctgc tgacgccact catcatccag ctttttatgg tacagcaagg
     4381 aaacaaccgc ttgattgctt agcgtagtga ggtaatcaat ctggaacaac ttgtggcgca
     4441 gaatgggatt attacgcaca cccgcaatca tcgccgtcat caactggttg ataagttcac
     4501 tggcggcggg gaagctatcc acacggatac gacttttggt ttgttgatcg aaaatgatgt
     4561 ggtacaggtc atcgccatcg tgccagatgc ggaactccgc acgcatccga taatgactga
     4621 ccggcgagcg aaacacttcc ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac
     4681 gtaccacttt ttcggctaac tgcgcttcat actgttctgt tggaaggtgt tcgggggtca
     4741 tgatgtatcc tgaaaaatta aagtacgcgg cgattgtagg gattgctcat cagatgtcca
     4801 gatcttgatg aattcctatt tgtgagctac gtctggacag taacttgtta caacctgtag
     4861 catccacttg ccggtcctgt gagttaatag ggaatccagt gcgaatctgg agctgacgcg
     4921 cagcggtaag gaaaggtgcg atgattgcgt tatgcggaca ctgccatccg gtgggaagtc
     4981 atcatctctt agtatcttag atacccctcc aagcccgaag acctgccggc caacgtcgca
     5041 tctggttctc atcatcgcgt aatattgatg aaacctgcgg catccttctt ctattgtgga
     5101 tgctttacaa tgattaaaaa agcttcgctg ctgacggcgt gttccgtcac ggcattttcc
     5161 gcttgggcac aggataccag cccggatact ctcgtcgtta ctgctaaccg ttttgaacaa
     5221 ccgcgcagca ctgtgcttgc gccaactact gttgtgacgc gtcaggatat cgaccgctgg
     5281 cagtcgacct cggtcaatga tgtgctgcgc cgtcttccgg gcgtggatat cacccaaaac
     5341 ggcggttcag gtcagctctc atctattttt attcgcggta caaatgccag tcatgtgttg
     5401 gtgttaattg atggcgtccg cctgaatttg gcgggggtga gtggttctgc tgatcttagt
     5461 cagttcccta ttgcgcttgt ccagcgtgtt gaatatatcc gtgggccgcg ctccgctgtt
     5521 tatggttcag atgcaatagg cggggtcgtg aatatcatca cgacgcgcga tcaccccgga
     5581 acggaaattt cagcagggtg gggaagcaat agttatcaga actatgatgt ctctacacag
     5641 caacaactgg gggataagac acgggtaacg ctgttgggcg attatgccca tactcatggt
     5701 tatgatgttg tcgcctatgg taataccgga acgcaagcgc agccagataa cgatggtttt
     5761 ttaagtaaaa cgctttatgg cgcgctggag cataatttta ctgatgtctg gagcggcttt
     5821 gtgcgcggct atggctatga taatcgtacc aattatgacg catattattc tcccggcttg
     5881 ccactggtcg atacccgtaa actctatagc caaagttggg atgccggact gcgctataac
     5941 ggcgaactga ttaaatcaca actgatttcc agctacagcc atagcaaaga ttacaactac
     6001 gatccgcatt atggtcgtta tgattcgtcc gcgacgctcg atgagatgaa acagtacacc
     6061 gtccagtggg caaacaacat catcattggc cacggtaata ttggtgcggg tgttgactgg
     6121 cagaagcaga gcacggcacc gggcacagct tatgttgagg atggatatga tcaacgtaat
     6181 accggcatct atctgactgg gctgcaacaa gtcggcgatt ttacttttga aggcgcagga
     6241 cgcagcgacg ataattctca gtttggtcgt catggtacct ggcaaaccag cgccggttgg
     6301 gagttcatcg aaggttatcg ctttattgct tcctacggaa catcttataa ggcaccaaat
     6361 cttgggcaac tgtatggcac ctacggtaat ccgaatctga atcctgagaa aagcaaacaa
     6421 tgggaaggcg cgtttgaagg cttaactgct ggggttaact ggcgtatttc cggatatcgt
     6481 aacgatgtca gtgacttgat cgattatgat gatcacaccc tgaaatatta caacgaaggg
     6541 aaagcgcgga ttaagggtgt cgaggcgacc gctaattttg ataccggacc actgacgcat
     6601 actgtgagtt atgattatgt tgatgcgcgc aatgcaatta ctgacacgcc gttgttacgc
     6661 cgtgctaaac agcaggtgaa ataccagctc gactggcagt tgtatgactt cgactggggg
     6721 attacttatc agtatttagg cactcgctat gataaggatt actcatctta tccttatcaa
     6781 accgttaaaa tgggcggtgt gagcttgtgg gatcttgcgg ttgcgtatcc ggtcacctct
     6841 cacctgacag ttcgtggtaa aatagccaac ctgttcgaca aagattatga gacagtctat
     6901 ggctaccaaa ctgcaggacg ggaatacacc ttgtctggca gctacacctt ctgaaccacg
     6961 tcccaccgtg ctggtgtttg actccggcgt cggtgggttg tcggtctatg acgagatccg
     7021 gcatctctta ccggatctcc attacattta tgctttcgat aacgtcgctt tcccgtatgg
     7081 cgaaaaaagc gaagtgttta ttgttgagcg agtggtggaa attgtcaccg cagtgcaaga
     7141 acgttatccc cttgcgctgg ctgtggtcgc ttgcaacacc gccagtaccg tttcacttcc
     7201 tgcattacgc gaaaagttcg acttcccggt tgttggtgtc gtgccggcga ttaaacctgc
     7261 tgcacgtctg acggcaaatg gcattgtcgg attactggca acccgcggaa ctgttaaacg
     7321 ttcttatact catgagctga tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg
     7381 ctcggcagag atggttgagt tggctgaagc gaagctgcat ggcgaagatg tttctctgga
     7441 tgcgctaaaa cgtatcctac gcccgtggtt aagaatgaaa gagccgccag ataccgttgt
     7501 actggggtgt acccatttcc ctctactaca agaagaactg ttacaggtgc tgccagaggg
     7561 aacccggctg gtggattctg gcgcagcgat tgctcgccga acggcctggc tgttagaaca
     7621 tgaagccccg gatgcaaaat ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc
     7681 agaagctgaa caattattgc ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact
     7741 ggcagtttta ggctgatttg gttgaatgtt gcgcggtcag aaaattattt taaatttcct
     7801 cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac
     7861 acgccgccgg gtcagcgggg ttctcctgag aatctcaaca gagaaaagca aaaaaatgct
     7921 tgactctgta gcgggaaagc gtattatgca
//
LOCUS       DAZFRG010000213         7814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFRG010000213 DAZFRG010000000
VERSION     DAZFRG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.214"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..873
                     /gene="mipA"
                     /locus_tag="ACHKUC_003879"
     CDS             127..873
                     /gene="mipA"
                     /locus_tag="ACHKUC_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO6620056.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGIAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            963..1817
                     /gene="yeaE"
                     /locus_tag="ACHKUC_003880"
     CDS             963..1817
                     /gene="yeaE"
                     /locus_tag="ACHKUC_003880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO6620057.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1868..2752)
                     /locus_tag="ACHKUC_003881"
     CDS             complement(1868..2752)
                     /locus_tag="ACHKUC_003881"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO6620058.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPLCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVVLTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(2836..3831)
                     /gene="gapA"
                     /locus_tag="ACHKUC_003882"
     CDS             complement(2836..3831)
                     /gene="gapA"
                     /locus_tag="ACHKUC_003882"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6620059.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            4173..4586
                     /gene="msrB"
                     /locus_tag="ACHKUC_003883"
     CDS             4173..4586
                     /gene="msrB"
                     /locus_tag="ACHKUC_003883"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO6620060.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            4628..4900
                     /locus_tag="ACHKUC_003884"
     CDS             4628..4900
                     /locus_tag="ACHKUC_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO6620061.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            5270..6346
                     /locus_tag="ACHKUC_003885"
     CDS             5270..6346
                     /locus_tag="ACHKUC_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6620062.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            6373..7752
                     /locus_tag="ACHKUC_003886"
     CDS             6373..7752
                     /locus_tag="ACHKUC_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620063.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
BASE COUNT         1973 a         1803 c         1993 g         2045 t
ORIGIN      
        1 gtgtcatctg cgtcaggaaa attaaacagt tactataaaa aatgaaaacg taaaaaggtt
       61 gggtttcgat gtattgacgg gtaaactttg tcgcccgcta aacatttgtt taaggaatga
      121 ttaattgtga ccaaactcaa acttctggca cttggagtgc ttatcgcaac gtctgcaggc
      181 gtagcgcacg ctgaaggtaa attttccctg ggcgcaggcg taggtgtcgt tgaacaccca
      241 tataaagatt acgataccga tgtttaccca gtaccggtaa tcaactatga aggcgataac
      301 ttctggttcc gtggcttagg tggtggttac tacctgtgga atgacgcaac ggacaaactt
      361 tcaattaccg cttactggtc gccgctttac ttcaaagcta aagacagtgg cgatcaccaa
      421 atgcgtcacc tggatgaccg taagagcacc atgatggctg gtctgtctta tgctcacttt
      481 acccagtacg gttacctgcg taccaccctg gctggcgata ccctggataa cagcaacggc
      541 atcgtctggg atatggcctg gttgtatcgt tacaccaacg gtggcctgac cgtgactccg
      601 ggtattggtg tgcagtggaa cagcgaaaac cagaacgaat actattatgg cgtatcgcgc
      661 aaagagtccg ctcgcagcgg tctgcgtggc tataacccga acgacagctg gagcccttac
      721 ctggagctga gcgccagcta caacttcctc ggtgactgga gtgtttacgg tatcgcgcgc
      781 tacacccgtc tgtctgatga agttactgac agcccaatgg tggataaatc ctggactggc
      841 ctgatttcta ccgggatcac ctacaaattc tgataatgca tttttccagg gcattttgtg
      901 cataaaaaca gggcgtaatg ccctgttttg cattacggtg gtgcaatacc tgggaggaag
      961 caatgcaaca aaaaatgatt caatttagtg gcgatgtctc actgccagcc atagggcagg
     1021 gaacatggta tatgggcgaa gatgccagtc agcgtaaaac agaagttgct gcactacgcg
     1081 cgggcattga actcggttta accctcattg ataccgccga aatgtatgcc gatggcggtg
     1141 ccgaaaaagt ggttggggaa gcattaatcg gtctgcgtga gaacgtcttt ctcgtctcta
     1201 aagtctatcc gtggaatgct ggcgggcaaa aagcgataaa tgcatgcgaa gccagtttac
     1261 gccgtctcaa tactgattat ctcgatctct acttattaca ctggtctggc agtttcgctc
     1321 tcgaagagac tgtcgcagcg atggaaaaat tgatcgccca gggaaaaatc cgccgctggg
     1381 gcgtttctaa ccttgattat gccgatatgc aggaactctg gcagctgccg gggggaaatc
     1441 agtgtgccac taatcaggtg ctttaccatc tcggttcacg agggattgag tacgatctac
     1501 ttccctggtg ccagaaacag cagatgccgg tgatggctta cagtccatta gctcaggccg
     1561 ggcggttgcg caatggactg ttaaaaaacg cggtagtcaa cgaaattgca catgctcaca
     1621 atatcagtgc ggcacaagta ttgttggcgt gggtaatcag ccatcagggt gtgatggcga
     1681 tcccaaaagc ggcaactgtc gcacatgtcc aacaaaatgc ggctgtgctt gaggttgaac
     1741 tttcgtcagc ggaattagtt atgctggata aagcgtatcc ggcaccaaaa ggaaaaactg
     1801 cgctggatat ggtgtgatgt tgatacgtgc cggatgcatc gcacccggca cacaatatta
     1861 acgcaaatta acgtttcgca acgcgaatgg attgcgccag atgtgcaggt ttctctttgg
     1921 tgactttttg cgtttctgaa gcgtaagccg tttctacaca aacaaaggtc ttgtagccat
     1981 catccggcat atcgcccatg ctaactgaaa gcgccggtcc tgggttccag cctacaacgt
     2041 tcagatgatg ctggtggcct acggcgataa tacgattcag cgcttcgtca ttaattacgc
     2101 tgcaatcttg cggattcaga tacacgcgat cggtacggtc agggaaggtc tgaataccgt
     2161 cggtcagtac atcttctttc gcgtcattta ctttatcaat gaagcgatcg cctagtccac
     2221 tgacgcttac cttagcgata tcacccacgt taaagtaggt atgcagagca gaggtagttt
     2281 caaattcgcc gtgtgcttca agatcgattt cacaggtttt acccacgtgg aaatgcgcta
     2341 acagcgtaaa gtcgtgcggc cagaattttt tcgtctcttc gctttgtgtc aattcaaaag
     2401 ttagcactac accatcagca tcttcacgat gtgatttcag cgtccacggc aggttgcggg
     2461 caaaaccgtg cgcaggcaga ccttgttgtg cagccggacc aaaccacggc cagcaaagcg
     2521 gtatgccacc gcgaatggcg acgccatttt tgaacggtgt gttgttactt aaccacagaa
     2581 cttcttcttc acccgcaggt ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg
     2641 cttttacctg gggatgatcg accacaatga ggtccagttc atccagttta cgacgggaga
     2701 ggacagggga gatttgttcg atgaccggaa gggcaaaaat tttcttaatc atgacgcagt
     2761 cctttaactt cattttatca ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca
     2821 cagagtcatc tcaacttatt tggagatgtg agcgatcagg tccagaactt tgttggagta
     2881 accggtttcg ttgtcgtacc aggataccag tttcacgaag ttgtcgttca gagcgatacc
     2941 cgctttagca tcgaacacgg aagtgcaaac ttcgccgttg aaatcggtag atactacgtc
     3001 atcttcggtg tagcccagaa cgcctttcat ttcgccttca gcagcagctt taacggcagc
     3061 tttgatctgc tcgtaagttg cagctttttc cagacgaacg gtcaggtcaa ctacggatac
     3121 gttcggggtc ggaacgcgga acgccatacc agtcagtttg ccattcagtt ctggcagtac
     3181 tttacctaca gctttagcag caccggtaga agacgggatg atgttctggg aagcgccgcg
     3241 gccgccgcgc cagtctttgt gagacgggcc atcaacggtt ttctgagtag cggtagtagc
     3301 gtgaacggtg gtcatcagac cttcgatgat gccgaagtta tcgttgataa ctttagccag
     3361 cggagccagg cagttggtgg tgcaggaagc gttggaaacg atgtcctggc cagcatattt
     3421 gtcgaagtta gcgcctttaa cgaacatcgg agtgttgtct ttagacggac cagtcataac
     3481 cactttcttc gcaccagcgg tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc
     3541 agttgcttca gcgacaacgt caacaccaac ttcgtcccat ttcaggttag ccggatcacg
     3601 ttcagcggta acacggattt ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac
     3661 ggtaccgtcg aaacggccgt gagtggagtc atatttcagc atgtatgcca tgtaatcagc
     3721 gtctaacagg tcgttgattg caacgatctc gatgtcagaa cgtttctgag cagcacggaa
     3781 aacaatgcga ccgatacggc caaaaccgtt gatacctact ttgatagtca tatattccac
     3841 cagctatttg ttagtgaata aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc
     3901 aagcggaatc gtgtcaatca ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg
     3961 actcgcctca ctcttccttt gggcttgaga ccacatgggg tcggcgcccc gaattttaaa
     4021 gggcaattac gataaaaatg tgattttcat cacgatttag gtgcagccat ttcgtcgaga
     4081 tcaagtttag gacaaaattt tgccagttgg tgttaatgtt ttgttagaat cagtcacgta
     4141 atgtgagcac gattaaagtg agatgtgagc aaatggctaa taaaccttcg gcagaagaac
     4201 tgaaaaaaaa tttgtccgag atgcagttct acgtgacgca gaatcatggg acagaaccgc
     4261 catttacggg tcgtttactg cataacaagc gtgacggcgt atatcactgt ttgatctgcg
     4321 atgccccgct gtttcattcc caaaccaagt atgattccgg ctgtggctgg cccagcttct
     4381 acgaaccggt gagtgaagaa tctattcgtt atatcaaaga cttgtcgcat ggaatgcagc
     4441 gcatagaaat tcgttgcggt aactgcgatg cccatctggg ccatgtcttt cctgacgggc
     4501 cgcagccaac gggcgaacgt tattgtgtta actctgcttc tttacgcttc accgatggcg
     4561 aaaacggcga agaaatcaac ggttgaaaaa acgattcagc aacttattcc acaggagcgg
     4621 aaaaattatg aatcttgatg acattatcaa cagcatgacg cctgaggtat accagcgttt
     4681 gtcgaccgcc gttgaactgg ggaaatggcc tgatggcgtt acgttgaccg aggaacaaaa
     4741 agagaactgc ctgcaactgg tgatgctgtg gcaagcccgc cataataccg aagcacagca
     4801 tatgacaatt gacacaaatg gtcagatggt catgaagagc aaacagcagt taaaagaaga
     4861 ttttggtatc tcagcaaagc ctattgccat gtttaagtaa gtggtttatc ccctttttgc
     4921 cgcggttata aaggggatag ccaacaatga ccccacgact catctcaatt atctcgcccc
     4981 gacctggcta agtccctgtc cgatctcttg cagtcgcgtt cacacctgaa tgacttctcc
     5041 tgaacttaag tctcaggcct atttggccgg gaatccctct cgaatttgct ctttttgcgt
     5101 cccgccttcc atcaacctgt aacagcccca gccattttct gcgtaattgt gatctaagtt
     5161 gtaaaacgca gatcattatc tgtgttttac agatttgatg ttgacctcaa aaatcatgtg
     5221 ggtaagtatg atgaccatct aagcaaaaca aaacacagag gatgacgcaa tgaaagcact
     5281 ggctcggttt ggcaaggcct ttggcggcta caagatgatt gatgtcccac aacccatttg
     5341 tggcccggaa gatgtcgtga ttgaaattaa agccgcggca atctgcggcg cagacatgaa
     5401 gcactacaat gtcgatagcg gttcggatga gtttaactct atccgcggcc atgagttcgc
     5461 aggttgtatt gcgcaggttg gtgaaaaagt caaagactgg aaagtggggc aacgcgtcgt
     5521 gtcggataac agcggtcacg tttgcggcgt ttgcccggcc tgtgaacaag gtgattttct
     5581 gtgttgtaca gaaaaagtaa accttggtct ggataacaat acctggggcg gtggtttttc
     5641 caaatattgt cttgttcctg gtgaaattct caaaattcat cgtcatgcgt tgtgggaaat
     5701 ccctgatggt gttgattatg aggacgcagc cgtacttgac cctatctgta atgcctataa
     5761 atccatcgcg cagcaatcga aattccttcc aggtcaggat gttgtcgtca tcggcactgg
     5821 cccactcggg ctgttctccg tacaaatggc gcggattatg ggggcggtaa atatcgtcgt
     5881 cgttggtctg caagaagatg tggcggtccg cttcccggtt gcaaaagaac tgggtgcgac
     5941 ggcagtagta aatggttcta ccgaagatgt ggtggcgcgc tgccagcaaa tttgtggcaa
     6001 agacaatctg ggactggtga ttgaatgctc cggtgccaat atcgcactga aacaagccat
     6061 cgaaatgctc cgcccaaatg gggaagtagt acgcgttgga atgggcttca aacctcttga
     6121 tttctcgatt aatgacatta ccgcctggaa caaaagcatc attgggcata tggcctatga
     6181 ctccacctca tggcgtaacg ctctccggct attagccagc ggcgcgatca aagtcaaacc
     6241 gatgatcacg catcgtatcg gcctgtcgca atggcgcgaa gggtttgatg cgatggtcga
     6301 taaaactgca atcaaagtga tcatgactta cgactttgat gaataactac tctcctgaag
     6361 gaacgttatc gcatggaaca gataacaaaa ccgcattgtg gtgcacggct ggatcgctta
     6421 ccggattgcc gctggcattc gtcaatgttt gccatcgtcg cgtttggctt gcttgtctgc
     6481 tggagtaatg ccgttggtgg cttgatcctc gcgcagctga aagcgttggg ctggacagat
     6541 aattccacca ctgccacatt ctcagcaatc acgaccgccg gaatgtttct cggtgcgctg
     6601 gtgggcggca tcatcggtga caaaaccggt cgcagaaatg cgtttatcct ctatgaggcc
     6661 attcatattg cttcgatggt tgtcggtgct ttctcaccga atatggattt ccttattgcc
     6721 tgccgttttg tgatgggcgt tgggctggga gctttactgg ttacactgtt tgctggtttc
     6781 accgaatata tgcccggaag aaatcgcgga acgtggtcca gtcgggtttc ctttattggt
     6841 aactggtcat atccgctctg ttcattgatt gcgatgggac tcacgccgct gattagtgca
     6901 gagtggaact ggcgggtaca actgcttatc cctgcaatat tgtcacttat cgctacggcg
     6961 ctggcctggc gctactttcc tgaatccccg cgctggctgg aatcgcgcgg acgttatcag
     7021 gaagccgaga aagtcatgcg gagtatagaa gaaggcgtca tacgccagac gggtaaacct
     7081 ttgccgcctg tggttattgc tgatgacggt aaagcaccac aagcggtgcc gtattcagcc
     7141 ttactgacag gagtattact gaaacgcgtg atattaggct cttgtgtgct gattgccatg
     7201 aacgttgtgc agtacacact aattaactgg ttgccaacga tattcatgac ccagggaatt
     7261 aatttaaaag actcgattgt tttaaatacc atgagtatgt ttggcgcgcc atttggtatt
     7321 tttattgcca tgctggtgat ggataaaatt ccgcgtaaaa caatgggtgt ggggctatta
     7381 atcctgattg cggtgctcgg atatatctat tcactgcaaa ccagtatgtt gctcataacg
     7441 ctgattggtt tcttcctgat tactttcgtc tatatgtacg tttgctatgc ctcggcagtg
     7501 tatgtccctg agatctggcc gacagaggcc aaactccgtg gctccggtct ggcgaatgcg
     7561 gtagggcgaa tcagtggtat tgccgcgcct tatgccgttg cagtgctgct cagtagttat
     7621 ggcgtaacgg gagtctttat tcttctgggg gctgtttcaa ttattgtcgc aattgctatc
     7681 gccaccattg gaattgaaac caaaggtgtc tccgttgaaa gtttaagtat tgatgcagta
     7741 gccaataaat aatactggtg gattcaaaat gaaaaattca aaagcaatat tgcaggtgcc
     7801 gggcacaatg aaaa
//
LOCUS       DAZFRG010000214         7765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFRG010000214 DAZFRG010000000
VERSION     DAZFRG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.215"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..623)
                     /locus_tag="ACHKUC_003887"
     CDS             complement(<1..623)
                     /locus_tag="ACHKUC_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620064.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGV"
     gene            complement(639..1043)
                     /locus_tag="ACHKUC_003888"
     CDS             complement(639..1043)
                     /locus_tag="ACHKUC_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620065.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(1133..1270)
                     /locus_tag="ACHKUC_003889"
     CDS             complement(1133..1270)
                     /locus_tag="ACHKUC_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620066.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            1342..1545
                     /locus_tag="ACHKUC_003890"
     CDS             1342..1545
                     /locus_tag="ACHKUC_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6620067.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            1567..1917
                     /locus_tag="ACHKUC_003891"
     CDS             1567..1917
                     /locus_tag="ACHKUC_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6620068.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHIATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            1928..2206
                     /locus_tag="ACHKUC_003892"
     CDS             1928..2206
                     /locus_tag="ACHKUC_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620069.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            2218..2460
                     /locus_tag="ACHKUC_003893"
     CDS             2218..2460
                     /locus_tag="ACHKUC_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO6620070.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAEHAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            2457..2570
                     /locus_tag="ACHKUC_003894"
     CDS             2457..2570
                     /locus_tag="ACHKUC_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620071.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            2663..3079
                     /locus_tag="ACHKUC_003895"
     CDS             2663..3079
                     /locus_tag="ACHKUC_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620072.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TSEAAKIIEEQSKEIDTLKTQSTVAAMNPSSIGRRIYILGSAIMTQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            3103..3306
                     /locus_tag="ACHKUC_003896"
     CDS             3103..3306
                     /locus_tag="ACHKUC_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620073.1"
                     /translation="MAIKHFPVIRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRK"
     gene            3303..3569
                     /locus_tag="ACHKUC_003897"
     CDS             3303..3569
                     /locus_tag="ACHKUC_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6620074.1"
                     /translation="MTILDYITTHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            3566..3865
                     /locus_tag="ACHKUC_003898"
     CDS             3566..3865
                     /locus_tag="ACHKUC_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620075.1"
                     /translation="MSTTNHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDKVFDFFLNQ"
     gene            4188..4418
                     /locus_tag="ACHKUC_003899"
     CDS             4188..4418
                     /locus_tag="ACHKUC_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6620076.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLFRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADNLRELQQLFQQQNKLH"
     gene            4491..4880
                     /locus_tag="ACHKUC_003900"
     CDS             4491..4880
                     /locus_tag="ACHKUC_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO6620077.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            4877..7660
                     /locus_tag="ACHKUC_003901"
     CDS             4877..7660
                     /locus_tag="ACHKUC_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6620078.1"
                     /translation="MNEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDHRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLISRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPDEIETIVSHVCDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPETEDSARENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQQEERPALWPQLPDGVDVDEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVNSTEIRHNPISSYLK
                     QLGASDPEAAARLASTIQQDQNTMKTPVTVLSDMLRAIRDAEHAQRINETTERARHKA
                     GLLQDKGNSEKKK"
BASE COUNT         2153 a         2095 c         1833 g         1684 t
ORIGIN      
        1 acaccgccta ccaaaacccc tacgcagata aagaatatta tttcttttgg ataaagtcgg
       61 attaattctg aagcacgaag tcggacttct ggtaaggtcg aactctctgt gtggcttgat
      121 gccgattgtt gctctaacca cgacaatgca gactgtaact gagaacgagt aagatcgttt
      181 aaacgtcctg taccgaaatt gatatggcaa taccgcagaa gtttttgtcg aagtccacag
      241 tcttcactgt tacgtagtaa taaacttaca agagccttac aggcatcatg atctttacat
      301 cgctcaagca ttgcatgcag aaaactctcc gctgttttat attgatttac tgtcatatca
      361 tcaataccag ctacaccaat ctccgcatgt actttttgcc aaataataaa cgcttcagta
      421 ttgctagctt ctgcaatagc agcaaccaag ctatttagct ccttacgctg agccttaagc
      481 aaagggcgat cgtcatcatc attattcgaa gggattgcga tattgacggt atgagaacca
      541 tcatatcgct ctatctgaat attcttttcg tgaaaatcac gcccagcaac gcgattgttt
      601 gaaccgtttg agttgacggc catgtcacct ccctactatc acctaccctt agtttcgtta
      661 tagtcacgac cagcgatacg gttattacca ccagaaatat ttaactcacg tcctgatggc
      721 tgagtttcct tttcactgat cgcacctttt aaagccccaa tcaccgcgtt tttcacatct
      781 aacgaagctg ctcgaaagcg agtaatcaac tcctgctcat catcgttata agtttcaggt
      841 gagtgaattc ccaacacaac atactgaaca tcaaggccaa aacgagacag cgctgccaaa
      901 tacgcagcat caggaaagct atctcctttc tcatatctaa gctgagttag ctttttgact
      961 ccaccaatgt cgctcatggc aacttgacta agtcccaatc tttccctttc ctcacgcaac
     1021 cgctgaccaa tatcattttt catacaaaaa ccttgacagg tatctttttt gataccaaaa
     1081 tgatttcacg agctattaga tgatcacaat ataccactat gaaacaagtt cttcacgata
     1141 ccagatcacg cattccgcgt aacaccgcca caggtccaag actggcactt cggctgtccc
     1201 tcgaggagcg agccgtcatt gatgaaatgg cagctaaaga acaacgctca tcctctaaca
     1261 tggcgcgcat gatcttcctt cgcggcctag agctaaccca gaaagaacaa aacaaatctt
     1321 cctgatcagg aggctagtgg gatgtcaggt ataaccatca atatcaatgt gaatgccccc
     1381 tatgtatccc tgcagaaata tgcagagata acaggtatcc ctcttaatac atgcaaaaag
     1441 atgttggctg acggtcgaat tattatccgc cccaaacgcg ccaaaatgga aaagcctgaa
     1501 gtaaaccttg tggcgatgtt aaaagacgct ttggctaaca gctaaaacaa tgaacagagc
     1561 accatcatga aaaaaaacgc taataatcca tactccaaat ttcgtaatgg cgtagaacgc
     1621 catgtacacc acatcgctac cagtgcatca cgtagtaaca gtcgctataa cctgaacgag
     1681 acgcacgcaa caccggatgg ccacgctgta aaacaaatcg gcgagcatgc ctggctgatt
     1741 gagaaagctg gaatcgtggt ccacaaatgc ccacgcaatc catttaccgg aaaccgcatt
     1801 tttgcattga actgcggcga caatcacttc gggcaggatt tcacattata cgaagcactt
     1861 cgcacggttg atcgtctgct tcgcgggcaa agttttatta aacaggctga tttataacag
     1921 gtgctttatg accaaagagc atgcacaagg tgtatttatc cgttttattg attttcgcgg
     1981 tgaactgtta ttacgcgcat cagccattga cggagttgtt ccatccgaaa aaaacgcagc
     2041 tacttacgtt tatctgaacg gtacgcgcct gactgtggag cttccgtacc agaccgtacg
     2101 agaaatcatt agcgaagctg aaaaggcacg tcaggttaat ggcgatgaac cctatatcga
     2161 aattatttgt atggattcag aagctgaaat tcagaaagca gattaaaggg cgttgcgatg
     2221 ggcaaagaat ataaaactct cattaacaaa gcacttgaac gattttattt tcgcttaagt
     2281 gcatcaggcg ctcatgctga acatgcagcc cgtgattcat tgaccagggc aatccggagt
     2341 ttgtatgacg ttgcttttta cgctgatgat ctggatgcac tgaacgaact ttccgagctg
     2401 atctgtgccg cagaatgcgg ggaacacatt gaaccgtata agctggggaa tatcgcatga
     2461 gtatatttat ctcatggctt gttctgatta tttcggtggc ctgcgccatt gggattatgc
     2521 gaattattca ttcagtaaaa aagattgaac gctttttcac tgacaaataa cagcttaaac
     2581 aaaacaccag attaaacctg aaaacctgaa aactatccgc attcgcggag gtattcgcac
     2641 acgtaaataa cggagatata aaatgaacgc aaaagaaaaa aatattatca atacattaaa
     2701 aatagtgtca gcagagcaag acaagctgtc cagggcagcc cagaaagata atcaacatat
     2761 ggcagcgctt tacgcactga ccatcgcaat agccacctct gaagcagcca aaattattga
     2821 ggaacagagc aaagaaatcg acactcttaa aacacagtca acagttgcag ccatgaatcc
     2881 gtccagcatt ggacgccgca tttacattct tggttcggca ataatgacgc aatacaccat
     2941 tattgccgaa ctgcacggca aatacctgat aacgccttac cacacaaaag agtcagagct
     3001 tctgacaaat ctccgcctga tagaacgatc tcaagctgta ttcattgatg acgcgcaacg
     3061 tgccgtattt aacgcatagg gttactggac aaagggggcg caatggcaat taagcatttt
     3121 cccgtcattc gctttacctc cagagggcgc gaatacgagg tcgacgaacg cctgattacc
     3181 actatcgaca aacatcgttc ggaaaaggat gcacaccaca tctacctcac tgacggcact
     3241 tacttctgcg ccaccaacgt ggcgcgggtg aatcttatcc gacaggtaca ggagccacgc
     3301 aaatgaccat tctggactac atcactacac atccgggttg tagcggcgga gaaatcgccg
     3361 cagcactgaa caccccaacc acagctatta atgctgagtt acgccgactt tggcgcggcg
     3421 gcttagtcat cagaacaaac cgcagcacag gtggtcgcgc tcgcaaaact ggaggccagg
     3481 cttcttacca cgtaaacccg atgccgttcg ggtgtagcaa tccacttact cacatgttta
     3541 accagctact gaaggaagcc agaacatgag caccaccaac caccagaagc tacgcgaact
     3601 ggcatttgcc ctgcaacgaa tggcaactcc tcaaaaatta ctggcatttc gcgcaatgct
     3661 ctcgccgtct gctgtgctgg cactgctgga tcagctggag cacgccagaa ccacggctcc
     3721 tgccattcgc ctgacgctcc atcatgaaat cgctgatttc tgcgcaacgc tgggggcacc
     3781 tggcgaaccg gaaacgccgg aagcaatgca gcaagagctg ctgcaacgca ttgacaaggt
     3841 ttttgatttt tttctgaacc agtaagaagc cagaacatgc acacacaaaa aaaccgcttg
     3901 ccatgccgca atcggtcagg ttacatttcc gctgcacctc ataaaacggg tgccgggatt
     3961 ctcaacccga tacagagcaa agcgcataac cgcgccagcg gtttttttgt gcgtactgta
     4021 ttgccacgtc tttttcgcgt cagaattatg gcggggcgta cggggccgac ttcggtcggg
     4081 ccggattctt tgctctccgg tgttgagaac cctgtacgtc tcgccacccc gagattctca
     4141 actctggatg gtgagctatt tctatcaccg agcaaagagg ccacaccatg gcaaaccgca
     4201 aacaacagcg cgcatacgct gcgcgtcgtc acatccagac tgaaatcaac cgtagacttt
     4261 tccgcgcatc acgcgtcgcg caaatcatgc acatcaatat gctgcatgag cgcagccacg
     4321 cgctatcgaa catctattcc gcctctgttt tcagctatct ggcggataat ctgcgcgagc
     4381 tgcaacaact cttccagcag caaaacaaac tccattaatt cctgttccgg gcctttcctg
     4441 caccttgcgg cgggaggcct tcgcacatct gtagtaaaga gaattgcagc atgattgacg
     4501 ctcatgactt cacaagatgg gtgcgcacac aggacacccg tctggctccc gttcttcagg
     4561 gattatttga tctctacatc cgtggtcgtg acaacagagc acgcaccaca aaaccggaga
     4621 atgcagacac cctttatttc acagtagacg actgctaccg cgtggacttc acaccacacg
     4681 ggctggcgtt gcactgcctg acaccacacg gagaatcact gctggcgtat tacgactccc
     4741 cggcctccgt atttgcggca atgctggcgc atcgcactgc tggcgggtgt gcctcgctga
     4801 gtgaatacac cgctgaattt aaccgccttt ctgccctgtt ctcgcaggag tggcagcgcg
     4861 tgacgggata ccagccatga atgagtttgc atggagctgg aatgaaccac ggccagccat
     4921 tgatccggcc agatttacgg agcacaggca ggaaactgaa accgacctgc aacgcgccat
     4981 ccgttactac cttgaggcag acaaaaaggc tctggaagaa caggaagcga aggaggaagc
     5041 ctttttcgca caatccaccg tgggtaaaaa actcatggca tcccttgagg aagccggaca
     5101 gcgtgaaaag ctggcacaaa gcatcatcag caagcgtcag gcaacagaac aagacccggt
     5161 ggcccgtgct tttgccacac tgaaggtgct tcccgtttat ctgcgtgaac ctctgagccg
     5221 ccacctctct ttcctgcgca aaaaacagga agccgatcgc cagaaaggca aaaagagctg
     5281 gcaggctgaa cgctacgcgc gcggaaccct gcgcaaaata ttcgaacgtc tggaccgcac
     5341 cgatcaccgc tggctgacac cgggttatcg ctcccttgcc ggacgcgaac gcctggacga
     5401 tttgctttac ctgccgcagc tcaacaaaca ccagatacag acgctggcca ccatgacggc
     5461 ggcgatgttc agcagcacct tcgaaaaact ctgcgatggc tttggcgcga ctgatggcga
     5521 actgaccatg gatgtaacgc tgaaggcgta tcagatgctg gcccgcatgg cgttacacct
     5581 gcacgccatg cctccacatt atgacgcact gacaacagac aaagaccgga ggaacgaacc
     5641 ggacacggag ctgctgccgg gcgcaatcct tcgcttgacc tgtgcggaat ggtggaaacg
     5701 caaactgtgg ctgttacgtt gtgagtggcg ggaagaacaa ctccgcgccg cctgtctgat
     5761 ttccagaaaa acatccccct atctgagcca ggacgcgtta agcgagtttc gcgcacagcg
     5821 cgagaaaaca cgcgatttcc tgaaaagttt catgctggaa aatgaagacg ggttcacgat
     5881 tgatctcgag acggtgtatt acgcgggagt aagtaacccg gttcaccgta aggcagaaat
     5941 gatggccacc atgaagggac tggaacttct ggccgaagcc cgtggcgaca gagcggtgtt
     6001 tctgaccgtc acctgcccgt caaaatacca cgcaacaacg gagaacggtc atccgaaccc
     6061 caaatggaac ggggccacca tgcgcgactc cagcgattac ctggttaaca cgttttttgc
     6121 ggcggtccgc aaaaaactga accgcgacgg cctgcgctgg tatggcatcc gcacggtgga
     6181 gcctcaccat gacggcactg tgcactggca tatgatggtc tttgcacatc cggacgagat
     6241 tgaaaccatc gtgtcccacg tctgcgatat tgccattcag gaggaccgcc acgagctggg
     6301 cgatgacata actccgcgtt ttaaggcgga gtatgtcgac ggctcaaaag gcacaccaac
     6361 cagctacatc gccacctaca tcggaaagaa cctggacagc cgcgccgtgg atggcatcga
     6421 cccgaaaacg ggcaagccac gcgttgacca cgaaaccgga aaatcaatgg ccgagagcgt
     6481 ggagcgcgcc atcggctggg cgcgccttca ccgggtccgt cagttccagt tctttggcat
     6541 cccctcccgt caggtgtggc gtgaactgcg ccgccttgcc agccagatgg cacgcaatcc
     6601 ggaaggcccg caacggctga aagatgatgc aatggatgcg gtactcgctg ccgccgatgc
     6661 cgggtgtttt gccacctaca ttgaaaaaca gggtggcgta cttgttccac gcaaagacta
     6721 cctgattcgc accgcctacg acctcgcaga tgagctgaac gattacggcg aacagagcgt
     6781 acagatttac gggatctggt cgccactcat cggggaatcc tcccgtgtgt gcacgcatcc
     6841 ggataactgg aagctggtaa gacgtaaacc ggaaacggaa gacagcgccc gcgaaaatgg
     6901 ttttgacctt cagggcggcc ctgccgcccc ttggactcgt ggcaataact gtccccgtgt
     6961 acaggaaacg gacaacaacg ggacagaaca gcaggaagaa cggccagcac tgtggccaca
     7021 gcttcctgac ggcgttgatg tggatgaatg gatgcgctca ctgaaacggc acgaacgccg
     7081 ggcgctgatg cgttcgcttc gtgacaaaca ggcaaaaaac agcagtgatg aaatgcagag
     7141 ctggacacag agccgcaaac agcagcggcc tttgcctgat aaccacgaat tactcgctaa
     7201 agaatggcgg gaatctgccg aatctctcgg cctgcatatc ggtgaacagc agatgcagca
     7261 cctgttacga ggcggcagtc tgtacgttga cggcagcatc attgcaccgc agggatttga
     7321 aattgtacgc aaaccagata cccgaccgga cagccgaatc acgcagctct ggcagcgcct
     7381 gagccgtaat cacggcgtaa acagcacgga aatccgccat aacccgattt ccagctatct
     7441 gaaacagctc ggggcatcag accctgaagc cgctgcacgc ctggcatcca caattcagca
     7501 ggaccagaac accatgaaaa ctcccgttac cgtgctttct gacatgctgc gcgccatccg
     7561 tgacgcagag cacgcacaga gaatcaatga aaccactgaa cgcgcacgtc acaaggcggg
     7621 tctgttgcag gacaaaggca atagcgagaa gaaaaaataa ccaagaaaga aatcatcaat
     7681 tgtatgaagg tagagtttag acatggtaag gacgttccat gaggcacact gtaatttcat
     7741 atagtattcc tcttccctgt ttgat
//
LOCUS       DAZFRG010000215         7749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFRG010000215 DAZFRG010000000
VERSION     DAZFRG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.216"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            669..1757
                     /locus_tag="ACHKUC_003902"
     CDS             669..1757
                     /locus_tag="ACHKUC_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO6620079.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDYEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1768..4047
                     /locus_tag="ACHKUC_003903"
                     /pseudo
     CDS             1768..4047
                     /locus_tag="ACHKUC_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            4040..5176
                     /locus_tag="ACHKUC_003904"
     CDS             4040..5176
                     /locus_tag="ACHKUC_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6620080.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5173..7176
                     /locus_tag="ACHKUC_003905"
     CDS             5173..7176
                     /locus_tag="ACHKUC_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO6620081.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDSAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLFATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            7301..>7749
                     /locus_tag="ACHKUC_003906"
     CDS             7301..>7749
                     /locus_tag="ACHKUC_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO6620082.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGF"
BASE COUNT         1849 a         2027 c         2058 g         1815 t
ORIGIN      
        1 ataagcgagt gaatccccag gagcttacta aagtaagtga caggggtgaa cgaacgcagt
       61 cgcagcacat gcaacttgaa gtatggcgag taaagcttta ttaaaggggg aaacggtatt
      121 cccccttaga tgcgtttcaa tttgcccgtt acttcaacaa atcaatcaac cgtaacagag
      181 ccactaatag ctccagtaca gcaatgagca cggttaactt ttgtgtcagc gtgtccatcc
      241 gctctcctta ttaaggagcg caacaccagc accttaccct tacactgcct gtcgatactg
      301 gtttacgtat gttggtacga aacgttgctc tccggatcag ggcaccgcct gcggcaccac
      361 ccgtccagca gccaggactt aaaattggtc cgatccggca caacctccca acgggcagga
      421 ttataaatct cattcactga attgcaatgg agtgtggata tggttataat ttattcgttg
      481 gtacgaaatg ttgcacaatc agcaagttag cgtcattgta gtaaataaaa gaacctgcct
      541 caccagcagg tttttttatt tactgtgatc tgctttccag atatttttcg ctcaaacaac
      601 taatgcggca aacatttatt gcgcgtaaaa tatcgtttat ttcattaata catttcaggg
      661 atgaatatat gtcaccgcag aataatcatc tgcagcgtcc gcctgccgct gtgttatacg
      721 ccgatgaact ggcaaaatta aaacaaaatg atcacgcacc ttgcccgccc ggttggcagt
      781 taagtttgcc tgcggcccgc gcttttatcc ttggcgatag cgcgcaaaat attagccgta
      841 aagtcgtgat cagtccctcc gctgtcgaac gcatgttagt cactctcgct accgggcgcg
      901 gtttgatgtt ggtgggggaa cctggcaccg caaaatcgct cctgtctgaa ttactggcaa
      961 ccgctattag cggcgatgcc gggttaacca tccagggcgg cgcatctacc accgaagatc
     1021 aaatcaagta cggctggaac tacgccctgc ttatcaacca tggtccgtca acggaagcgc
     1081 tggtccctgc cccgctttac cagggaatgc gcgacggcaa aattgtccgt tttgaagaga
     1141 taacgcgtac accgctggaa gtacaagact gtctgttagg tatgctttct gacagggtaa
     1201 tgacggtgcc ggaactcact ggtgaagcca gccagctcta tgcacgggaa ggctttaata
     1261 ttattgccac tgccaatacc cgcgatcgcg gcgtcaatga aatgagtgct gcgctaaagc
     1321 gtcgtttcga ttttgaaact gtctttccga ttatggattt cgcccaggag ctggagcttg
     1381 tcgccagtgc ttcggcgcgt ttgctggccc atagcggcat tccgcataaa gtgcctgacg
     1441 cggtactgga gttactggtg cgcacctttc gcgatctgcg tgccaacggc gaaaagaaaa
     1501 cgtcaatgga tacactaaca gcgattatgt ccaccgctga agccgtgaac gtggcccacg
     1561 cggtgggcgt ccgcgcctgg tttttagcga atcgcgcagg tgagccagcc gatctggtgg
     1621 actgtattgc cggaaccatc gtcaaagatt acgaagaaga tcgcgcgcgc ctgcgccgtt
     1681 acttcgaaca acgtgttgct acacataaag aagctcactg gcaggcttat tatcaagctc
     1741 gccaccgcct gccgtgagga aagatgcatg agcgagccgt taattgtcgg catccggcat
     1801 catagcccgg cctgcgcccg gctggtgaaa ttgttaatcg aaagccagcg gccacgatac
     1861 gtattgattg aaggcccggc tgattttaat gaccgggtgg acgaactgtt tttagcccac
     1921 cagcttccgg tagctattta cagttattgc cagtatcagg acggtgcggc ccccgggcgt
     1981 ggtgcctgga cgccatttgc tgaattttcg ccggagtggc aggcgctaca agccgcacgt
     2041 cgtattcagg cacaaactta cttcatcgat ttgccttgct gggcgcagag tgaagaagaa
     2101 gacgaatcac ccgatacgca agatgacagc cagaccttac tgctgcgtgc cacctgcatg
     2161 gataacagcg ataccctgtg ggatcactta ttcgaagatg aaagccagca aactgcatta
     2221 ccctctacgc tggcgcacta ttttgctcaa ctgcggagcg attcccccgg agatgcgctc
     2281 aatcgtcagc gcgaagcctt tatggctcgc tggattacat gggcgatgca gcaaaacaat
     2341 ggcgacgtgt tagtcgtctg cggcggctgg cacgctcctg cactggcaaa aatgtggcgc
     2401 gaatgcccgc aggaaattaa caagccagaa ttgccctcgc tggcagatgc cgttactggt
     2461 tgttatctca caccctacag tgaaaagcgc cttgatgtgc tggcaggata cctttccgga
     2521 atgcctgccc cggtctggca aaactggtgc tggcagtgtg gcttacagca ggcaggtgaa
     2581 caactgctga aaacggttct tacccgtttg cgccagcaca aattgcccgc ttctaccgct
     2641 gatatggctg ctgctcatct gcatgctatg gcactggcac agttgcgcgg ccatacacta
     2701 cctctacgca ctgactggct ggatgccata gctggttcgc tgattaaaga agccctgaat
     2761 gcgccgttgc cgtggagcta ccgcggagtc attaatcccg ataccgatcc gattctggta
     2821 acgttgatag acacattagc gggtgacgga ttcggtaaac ttgccccttc tacgtcacaa
     2881 ccgcctctgc caaaagatgt cacctgcgaa ctggaacgta ccggaatctc tcttccggcg
     2941 gagcttacct taaatcgctt tacccccgat ggactggcgc aaagtcaggt gttacatcgg
     3001 ttggcaatac tggagatccc aggggttgtg cgccagcagg gaagtacact gtcacttgca
     3061 ggcaacggtg aagaacgctg gaaattaacc cggccactta gccagcatgc ggcattgatt
     3121 gaagccgcat gctttggcgc cacactctag gaagccgcac gccataaatt agaagccgat
     3181 atgctggacg cgggtggaat cggctgtatc accacatgtc ttagccaggc agcgttagcg
     3241 ggtctggcgt ctttcagtca gcaattactg gaacaactca cattattaat cgcccaggaa
     3301 aatcaattcg ccgaaatggg ccaggcgctg gaagtgctat atgccttatg gcggctggat
     3361 gaaattagcg gtatgcaagg cgcgcagata ttacaaacga cattatgcgc ggccatcgat
     3421 cgcacgctgt ggctatgtga atccaacggc agaccggatg aaaaggagtt tcacactcac
     3481 ctacatagct ggcaggcgct ttgccatatc ctgcgcgatc tacatagcgg cgttaattta
     3541 cccggcgttt cgctttctgc ggcagtagcc ttactggagc gccgcagtca ggcgattcat
     3601 gccccggcgc tggatcgcgg cgcgactctt ggcgcactga tgcgtctgga acatcccaac
     3661 gccagtgccg aaacggcgct gacgatgctg gcgcagttat ccccggcaca atccggcgag
     3721 gcgctgcacg gtttgctggc attagcccgt catcaactgg cctgtcagcc ggtatttatc
     3781 gccggtttca gcagtcattt aaatcaactg agtgatgccg attttatcaa tgccctgccc
     3841 gatttacgcg cggcgatggc ctggctacca cctcgagaac gcgggacgct ggcgcatcag
     3901 gtgcttgagc attatcaact ggtgcaactt cccgtttcgg cactgcaaat gccgttgcat
     3961 tgtccaccac aagacattgc acatcatcaa caactcgaac agcaggcact ggcatcgctg
     4021 caacactggg gagttttcca tgtctgaact gaacgatctt ctgaccaccc gtgagctaca
     4081 acgctggcga ttaattcttg gcgaagcggc agaaacgacg ctttgtgggc tggatgacaa
     4141 cgcccggcag atagaccacg cgctggagtg gctgtatggg cgcgatcctg aacggctcca
     4201 gcgtggtgaa cgttacggtg gattaggtcg ttcaaatctc accacccctg agtggatcaa
     4261 cagcattcac acgctgtttc cgcaacaggt gattgagcgg ctggaaagcg atgccgtgct
     4321 gcgctacggc attgaagatg tggtgacgaa tctcgacgtg ctggaacgta tgcagccttc
     4381 tgaaagcctg ctacgcgccg ttttgcacac caaacatctg atgaatcccg aagtactggc
     4441 tgccgcccgc cggatagtgc accaggttgt tgaagagatt atggcgcgac tggcaaagga
     4501 agttcgtcag gctttttctg gagtacgcga tcgccgtcgc cgctcattta tttcactggc
     4561 gcgaaacttt gatttcaaaa gtactctgcg cgccaacctg caacactggc acccgcaaca
     4621 cggcaagttg tatatcgaat ccccccgctt taacagccgc attaaacgcc atagcgaaca
     4681 atggcaactg gtcctactgg ttgatcaaag cggatcaatg gtggactcgg tgatccactc
     4741 cgcagtgatg gcggcctgtt tatggcagtt acccggcatt cgtacccatc tggtcgcttt
     4801 tgacacaagt gtcgtcgatc tcaccgccga cgttgccgat ccggtagagc tattaatgaa
     4861 agtgcaatta ggcggcggga ccaatatcgc cagtgccgtg gagtatggtc ggcaacttat
     4921 tgaacaacct gcaaaaagtg tcattattct cgtgagcgat ttttatgaag gtgggtcatc
     4981 atcattgctg acgcatcagg tgaaaaagtg tgtccagagc ggcatcaaag tgctggggct
     5041 ggcagcgctc gacagcaccg caacgccttg ctatgaccac gatacggccc aggcgctggt
     5101 taatgtcggc gcacaaatag ccgcaatgac accgggcgag ctggcttcct ggcttgcgga
     5161 gaatcttcag tcatgaattc actacgtccg gaattattag aactgacacc acaggcactg
     5221 acggcgttaa gcaatgccgg ttttgttaag cgcagcctta aggaactgga aaatggcaac
     5281 gtcccggaaa tcagtcatga gaacggcgct ttaatcgcca cgttcagtga cggtgtccgt
     5341 acccagctgg cgaacggcca ggcactgaaa gaggctcagt gcacctgcgg ggccagcggc
     5401 atgtgccgtc atcgcgtgat gctggtgtta agttatcaac gactttgtgc caccgcccag
     5461 cctacggaaa aaaaagaaga agagtgggat tcggcaatct ggctgaaaga actggctacc
     5521 ttgcccgatg ccacccgcaa acgcgcgcag gcgctggtcg ctaaaggcat caccattgag
     5581 ttgttctgtg cgcctggcga aattccctct gcccgattac cgatgagcga tgtgcgtttt
     5641 tattcccgca gcagcattcg cttcgcacgc tgtgattgca ttgaaggcac actttgcgaa
     5701 catgtggtac tggctgtgca ggccttcgtc gaggccaaaa ctcagcaagc ggaatttact
     5761 catttaatct ggcagatgcg cagtgaacac gtcacatcat ctgatgatcc gtttgccagc
     5821 gaagaaggca aaacgtgtcg tcaatatgtt cagcaattaa gccaggcatt atggctgggc
     5881 gggataagtc aaccgccaat ccattacgag gccgctttca gtcgcgcgca acaagcggcg
     5941 gaacgctgca actggcgatg ggtgagtgaa tcgctacggc aattgcgcgc cagtgttgat
     6001 gccttccacg cccgtgccag ccactaccat gccggagaat gcttacgtca gcttgcggca
     6061 ttaaacagtc gattaaattg cgtacaagag atggcgcgac gcgacagtat cggtgaagtt
     6121 cctcccatgc cgtggcgcac ggtcgttggc gctggtattg ccggagaagc aaaacttgat
     6181 catctgcggc tggtgtcttt aggtatgcgt tgctggcagg atattgagca gtatggttta
     6241 cgcatctggt ttaccgatcc cgacacagga agtattttgc atctttcgcg tagttggcag
     6301 agaagcgaac aggaaaactc accggcagcg acgcgtcggc tgtttagttt tcaggctggc
     6361 gcactggcag gtgggcaaat tgtttcacaa gcagcaaaac gcagtgccga tggcgagctg
     6421 ctgttcgcga cccgtaaccg cttgagtagc gttgttccac tgtcgccaga cgcctggcaa
     6481 atgttgagcg cgccgttgcg ccagccgggc attgtggctt tgcgggaata tttacgccag
     6541 cgtccccccg cctgcatacg accactcaac caggtcgata acttatttat tctgccggtc
     6601 gcggagtgta tttcgctcgg ttgggatagc agccgccaga cactggatgc gcaggtaatc
     6661 agcggcgaag gggaagataa tgtgctgacg ttatcccttc cggcatctgc ctgttctcct
     6721 tttgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt gagcctggtt
     6781 tctggctttg tcagttttgt agaggggcaa ttgacactgg aaccacgggt gatgatgaca
     6841 aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg tggcaccgct accttctgcc
     6901 agcgttctgc ctgtgccgtc taccgctcat cagttgctga tgcgctgcca ggcgttactt
     6961 attcaactgc tccataacgg ctggcgctat caggaacaga gtgctattgg tcaggcagag
     7021 ttgctggcga atgacctcac cgcggtgggt ttttatcggc tggcacatgt gttgggacaa
     7081 tttcgtaata cagaaagcga ggcacgggta gaagcaatga ataacggtgt tttgctttgc
     7141 gaacaattat tccccatgct tcagcaacaa ggatgaaata gtgcttttta ttaagagttc
     7201 tactcctgtt ccggactgct cacgccacag tattaggcat atcctatata gcccctggtg
     7261 agagtcacca gttccttgat taaataaaat ggagttttac atgaaggctt tcaataagct
     7321 gttttccctt gttgttgcat ctgttctggt tttctctctt gctggctgcg gtgacaaaga
     7381 agaatcaaag aaattcagcg ccaatctgaa cggcactgaa attgccatta cctatgtcta
     7441 caaaggtgac aaggtgctta agcaatcttc tgaaaccaaa attcaatttg cctccattgg
     7501 tgcaaccacc aaagaagatg ccgccaggac acttgagccg ttaagcgcta aatacaaaaa
     7561 catcgcgggt gttgaagaaa aattaaccta taccgatacc tacgcgcagg aaaacgtgac
     7621 tattgatatg gaaaaagtgg attttaaagc cctgcagggt atttcaggaa tcaacgtttc
     7681 tgctgaagat gccaaaaaag gtatcactat ggcgcaaatg gaactggtga tgaaagccgc
     7741 tggttttaa
//
LOCUS       DAZFRG010000216         7721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFRG010000216 DAZFRG010000000
VERSION     DAZFRG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.217"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1436
                     /gene="sad"
                     /locus_tag="ACHKUC_003907"
     CDS             48..1436
                     /gene="sad"
                     /locus_tag="ACHKUC_003907"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO6620083.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKTAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITTAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            1500..2426
                     /gene="glsB"
                     /locus_tag="ACHKUC_003908"
     CDS             1500..2426
                     /gene="glsB"
                     /locus_tag="ACHKUC_003908"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO6620084.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            2426..2785
                     /gene="yneG"
                     /locus_tag="ACHKUC_003909"
     CDS             2426..2785
                     /gene="yneG"
                     /locus_tag="ACHKUC_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO6620085.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            2924..4342
                     /locus_tag="ACHKUC_003910"
     CDS             2924..4342
                     /locus_tag="ACHKUC_003910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6620086.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            4569..6020
                     /gene="uxaB"
                     /locus_tag="ACHKUC_003911"
     CDS             4569..6020
                     /gene="uxaB"
                     /locus_tag="ACHKUC_003911"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO6620087.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            6217..7131
                     /gene="yneE"
                     /locus_tag="ACHKUC_003912"
     CDS             6217..7131
                     /gene="yneE"
                     /locus_tag="ACHKUC_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO6620088.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAQYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            7196..7597
                     /gene="hipA"
                     /locus_tag="ACHKUC_003913"
                     /pseudo
     CDS             7196..7597
                     /gene="hipA"
                     /locus_tag="ACHKUC_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
BASE COUNT         1829 a         1772 c         2039 g         2081 t
ORIGIN      
        1 gtaaatctgc gtatcttcat accatgactc ataaaggaga taccccgatg accattactc
       61 cggcaactca tgcaatttcg ataaatcctg ccaccggtga acaactttct gtgctgccgt
      121 gggctggtgt tgacgatatc gaaaacgcgc ttcagctggc agcagcaggc tttcgcgact
      181 ggcgcgagac aaatatagat tatcgtgctg aaaaactgcg tgatatcggt aaggctctgc
      241 gcgcccgtag cgaagaaatg gcgcaaatga tcacccgtga aatgggcaaa ccaatcaacc
      301 aggcgcgcgc tgaagtggcg aaatcggcga atttgtgtga ctggtatgca gaacatggtc
      361 cggcaatgct gaaggcagaa cctacgctgg tggaaaatca gcaggcggtt attgagtatc
      421 gaccgttggg gacgattctg gcgattatgc cgtggaattt tccgttatgg caggtgatgc
      481 gtggcgctgt tcccatcatt cttgctggta acggctactt acttaaacat gcgccgaatg
      541 tgatgggctg tgcacagctc attgcccagg tgtttaaaga tgcgggtatc ccacaaggcg
      601 tatatggctg gctgaatgcc gacaacgacg gtgtcagtct gatgattaaa gactcgcgca
      661 ttgctgctgt cacagtgacc ggaagtgttc gtgctggagc ggctattggc gcacaggctg
      721 gagcggcatt gaaaaaatgc gtactggaac tgggtggttc ggatccgttt attgtgctta
      781 acgatgccga tctggaactg gcggtgaaaa cggcggtagc cggacgttat cagaataccg
      841 gacaggtttg tgcagcggca aaacgcttta tcatcgaaga gggaattgct tcggcattta
      901 ccgaacgttt tgtggcagct gcggcagcct tgaaaatggg cgatccccgt gatgaagaga
      961 acgctctcgg accaatggct cgttttgatt tacgtgatga gctgcatcat caggtggaaa
     1021 aaacactggc gcagggtgcg cgtttgttac tgggcgggga aaagatggct ggggcaggta
     1081 actactatcc gccaacggtt ctggcgaatg ttaccccaga aatgaccgcg tttcgggaag
     1141 aaatgtttgg ccccgttgcg gcaatcacca ctgcgaaaga tgcagaacat gcgctggaac
     1201 tggctaatga tagtgagttc ggcctttcag cgaccatttt caccaccgac gaaacacagg
     1261 ccagacagat ggcggcacgt ctggaatgcg gtggggtgtt tatcaatggt tattgtgcca
     1321 gcgatgcgcg agtcgccttt ggtggcgtga agaagagtgg ctttgggcgt gagctttccc
     1381 atttcggctt acacgaattc tgtaatatcc agacggtgtg gaaagaccgg atctgacgct
     1441 acatattcag ctctgatata ctcgcaggtc ttttcagacc tgcggtccag gagtagaaag
     1501 tggcagtcgc catggataat gcaattttag aaaacatctt gcggcaagtg cggccgctca
     1561 ttggtcaggg taaagtcgcg gattatattc cggcgctggc tacggtagac ggttcccgat
     1621 tggggattgc tatctgtacc gttgacggac agctttttca ggccggagac gcgcaagaac
     1681 gtttttccat tcagtctatt tccaaagtgc tgagtctcgt tgtcgccatg cgtcattact
     1741 ccgaagagga aatctggcaa cgcgtcggca aagatccgtc tggatcacca ttcaattctt
     1801 tagtgcaact ggaaatggag cagggtattc cgcgtaatcc gtttattaat gccggtgcgc
     1861 tggtggtctg cgatatgttg caagggcgat taagcgcacc tcggcaacgt atgctggaag
     1921 tcgtgcgcgg cttaagtggt gtgtctgata tttcctacga tacggtggta gcgcgttccg
     1981 aatttgaaca ttccgcgcga aatgcggcta tcgcctggct gatgaagtcg tttggcaatt
     2041 tccatcatga cgtgacaacc gttctgcaaa actactttca ttactgcgct ctgaaaatga
     2101 gctgtgtaga gctggcccgg acgtttgtct ttctggcgaa tcaggggaaa gctattcata
     2161 ttgatgaacc tgtggtgacg ccaatgcagg cgcggcaaat taacgcgctg atggcgacca
     2221 gcggtatgta ccagaacgcg ggggagtttg cctggcgggt gggactaccg gcaaaatctg
     2281 gcgttggtgg cggtattgtg gcgattgttc cgcatgaaat ggccatcgca gtctggagtc
     2341 cggaactgga tgatgccggt aactcgcttg cgggcattgc agtccttgaa caattgacga
     2401 aacaattagg gcgttcagtt tattaatgca gtctctcgat ccactcttcg cgcgtttatc
     2461 ccgttcaaaa tttcgctctc gctttcgttt gggtgtgaaa gagcgtcagt attgtctgga
     2521 gaaaggcgcg ccagtcattg aacaacatgc ggcggatttt gtcgctaaac gtcttgctcc
     2581 ggcattaccg gctaacgacg gtaagcaaac ccccatgcgc gggcatccgg tttttatcgc
     2641 tcaacacgcc accgcgacct gttgtcgcgg ttgtctggct aagtggcata acattcctca
     2701 gggggaagcg ttaagtgagg agcaacaacg ttatattgtg acggtaatct accactggct
     2761 ggttatacag atgaatcagc catgataatt acggaaatcg ataaaatggc attcattagg
     2821 tatctttagt tgccgccgtt gtcagcgaac atgctatcct ttctaagaat ttacttattg
     2881 cgttgagata attgccaccc gcaattgtat ggacagataa ttaatgcatg tacagcccat
     2941 ttcaactttc cgtttgttcc aggaaggtca tctgctacgt aatagcatcg ctatttttgt
     3001 gctaaccacg ctgttctatt ttattggtgc agagttacga ctggttcacg aactttctct
     3061 tttctggccg ctgaatggcg taatggcggg ggtgtttgcc cgctatgtct ggcttaatcg
     3121 actgcattac tatgcgatca gttatgtggc gatgctggtt tatgatgcca taaccaccga
     3181 atgggggctg gtttcactgg ctatcaattt ctccaatatg atgtttattg ttaccgtcgc
     3241 cttactggtc gcgcgggata agcgtctggg aaaaaataag tatgagccag taagtgcctt
     3301 acggctattt aattactgtc tgcttgccgc attattatgc gctattgtcg gggcgattgg
     3361 ttcggtcagt attgatagtc tggatttctg gcctttgctt gccgactggt ttagtgagca
     3421 attctcaacg ggcgtgttga tcgtgccttg tatgctgacg ttggcaattc ctggagtact
     3481 gccgcgcttt aaagcagagc agatgatgcc tgctatcgcg cttattgtgt cggttattgc
     3541 ctcggtagtc attggcggag cggggagtct ggcgtttccg ctccctgcat taatctggtg
     3601 tgcagtgcgc tatacgccgc aggtaacatg tctgttgacc tttgtcaccg gtgcggtgga
     3661 agtcgtactg gtggcaaatt cggtgattga tatctcggtc ggttcgccgt tctccattcc
     3721 gcaaatgttc tccgcacgtc tcggtattgc cacgatggcg atatgcccaa ttatggtttc
     3781 ctttagcgtg gcagcgatca attcgctaat gaagcaagtt gcgctgcgag ccgactttga
     3841 ttttctgact caggtttact cacggtccgg tctttatgag gcgctgaaaa gtccatcgct
     3901 gaaacagaca caacatctga cggtcatgct gcttgatatc gactatttca aaagcattaa
     3961 cgataactat ggacatgaat gtggcgataa agtgttaagc gtgtttgccc agcatattca
     4021 gaagattgtc ggtgataagg ggctggtggc gcgaatgggc ggcgaagaat ttgctgttgc
     4081 agtgccgtcg gtgaatcctg tcgatggcct gctaatggcg gaaaaaatcc gtaaaggcgt
     4141 tgaacttcaa ccgttcacct ggcaacaaaa aacgctttat ctcacggtaa gtattggcgt
     4201 cggtagtggt tgcgcatcgt accgaacgct gactgatgac tttaataaat tgatggtcga
     4261 agccgatacg tgtctgtatc gctcgaagaa agatgggcgc aaccgtacca gcactatgcg
     4321 ttacggtgag gaagttgtct gacatcttta tttactcaga gcgaattaaa tgcaatatgt
     4381 tctaccactt tatgatccaa gaaaaggcct gtttcctcga tcaaaccatg atccgcgcca
     4441 cacttttctc ataaaactgc tcattaatca tcaggagttg atcaaaacag gggttcggcg
     4501 tattaaagtt tgctaacctg tcgtaccaat tcaaacaggt tgtatgacta atcagaaggg
     4561 aacccattgt gaaaacacta aatcgtcgcg attttcccgg tgcacagtat ccagaacgta
     4621 tcattcagtt tggtgaaggt aacttcctgc gcgcctttgt tgactggcaa atcgatctcc
     4681 tgaatgagca caccgatctg aattctggcg tggtcgttgt tcgtccgatt gaaacttcat
     4741 tcccgccgtc actgagcacg caggatggtc tttataccac cattatccgt ggcctgaatg
     4801 agaagggtga agcggtcagc gacgcgcgcc tgattcgctc tgttaatcgt gaaatcagcg
     4861 tctacagtga atacgatgaa ttcctgaaac tggcgcacaa cccggaaatg cgctttgttt
     4921 tctctaacac caccgaagcg ggtatcagct accatgcggg cgataaattc gatgacgcgc
     4981 cagcggtaag ttatccggca aaactgaccc gtctgctgtt cgaacgtttt agccatttca
     5041 acggtgcgct ggataaaggt tggatcatca tcccgtgtga gttgatcgac tacaatggcg
     5101 acgcgttgcg tgaactggtg ctgcgctatg cgcaagagtg ggcactgcca gaagcattta
     5161 ttcaatggct ggatcaggct aacagcttct gttctacgct ggtggaccgt atcgttaccg
     5221 gctatccgcg tgatgaagtg gctaaactgg aagaagaact gggttatcac gatggtttcc
     5281 tcgacaccgc tgaacacttt tacctgtttg tgattcaggg gccgaaatcc ttagcgactg
     5341 aactgcgtct ggacaaatat ccgctcaacg tgctgattgt tgacgatatt aaaccgtata
     5401 aagaacgcaa agtggcaata ctcaacggtg cgcacaccgc gctggtgccg gtggcgttcc
     5461 aggcagggct ggataccgta ggtgaagcga tgaacgatgc tgaaatttgt gcattcgttg
     5521 aaaaagccat ctacgaagag attattccgg tactggattt gcctcgtgat gaactggaat
     5581 ctttcgccag tgcagtcacc ggtcgtttcc gtaatccgta cattaagcat cagctgctgt
     5641 ctatcgcgct taacggcatg accaagttcc gcacccgtat cctgccgcag ctgctggcag
     5701 ggcagaaggc aaacggcaca cttccggcgc gccttacttt cgcattagca gcattgattg
     5761 cgttttatcg cggtgagcgt aatggagaaa catatccggt acaggacgat gcccactggc
     5821 tggaacgtta tcagcaactc tggagccagc atcgtgaccg tgttatcggc acccaggaac
     5881 tggttgctat tgtgttggct gaaaaagacc actgggagca ggacctgacg caagtgcctg
     5941 gtctggtcga gcaggttgct aatgacctgg atgcgattct cgaaaaagga atgcgcgaag
     6001 ctgtacgtcc gttgtgctaa tactctgaac ccggtttcga ccgggtttct tatttcacta
     6061 tattagttac aacatcctat gtgcctttat cattaacagg tgccatatgc cacacctgta
     6121 aatgttgtaa gcttgtaggt aatttgtaag tagtgtcttg agaatgtgat cgtaatcacg
     6181 tttaataggg cgacttttcc acgcctgaag ataaccatga ttgttcgtcc acaacaacac
     6241 tggctgcgcc gtatttttgt ctggcacggt tcagtattat ccaagatatc ctcgcgctta
     6301 ctactcaatt ttctcttttc gatcgctgtt attttcatgc tgccctggta cacgcatttg
     6361 ggcattaaat tcaccctcgc accgttcagc attctcggtg tcgccatcgc catttttctt
     6421 ggttttcgta ataatgccgg gtacgcccgt tacgttgaag cgcgaaaact ttgggggcag
     6481 ttgatgattg cctcacggtc gttactgcgc gaggtaaaaa cgacattgcc agattcggca
     6541 agtgtaaggg agtttgcccg gctgcaaatc gccttcgctc actgtttacg catgacatta
     6601 cgcaaacaac cacaggcgga agtgctggct caatatctca agacggaaga tcttcagcgt
     6661 gtactggctt cgaactctcc agctaaccgt atcttgttaa taatggggga gtggttggcg
     6721 gttcagcgcc gcaatgggca gctttcagat atcctgttta ttagcctcaa cgatcggctt
     6781 aatgatattt cagcagttct ggcaggctgc gagcgcattg cctacacgcc aattcccttt
     6841 gcctacaccc tgattttgca tcgtaccgtt tatctgtttt gtatcatgct gccgttcgcg
     6901 ctggtcgtgg atctgcatta catgacgcct tttatctctg tgctgatttc ctacactttt
     6961 atttcgctgg attgtctggc ggaagaactg gaagatccct tcggtactga aaacaatgat
     7021 ttaccgctgg atgccatctg caacgctatt gaaattgacc tgttacagat gaacgatgaa
     7081 gcagaaattc cagcgaaaat tcttcccgat cgccattacc agctgacgtg ataattactc
     7141 cgtacggcgg gcaacaataa acagacgcgg aaatgccagc aatatctgtc cgttaacaga
     7201 tggccatgca aaaaacttct ccgtatttat tcaggctggc ggcagttatc ggctcacacc
     7261 attttatgac atcatttcgg catttccggt ccttggcggt acgggaatac acatcagcga
     7321 tctcaaactg gcaatggggc ttaacgcatc caaaggcaaa aaaacggcaa tcgataaaat
     7381 ttatccgcga cattttctgg cgacagcaaa ggtgctgaaa ttcccggaag tgcagatgca
     7441 tgaaatcctg agtgactttg ccagaatgat tccggcagca ctggataacg tgaagacttc
     7501 attaccgaca gattttccgg agaacgtggt gacggcagtt gaaaccaatg tgttgaggtt
     7561 gcacggtcgg ttaagccgag aatacggtat taagtaagat atggggtttt ggtcgttgtt
     7621 aatgagcaga acaatcatga accgccaacg actggattct cccttggttt atgttggtgc
     7681 gcataatgta tattatgtta aatttaattt gagtgatgcg a
//
LOCUS       DAZFRG010000217         7688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFRG010000217 DAZFRG010000000
VERSION     DAZFRG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.218"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..98
                     /gene="hisL"
                     /locus_tag="ACHKUC_003914"
     CDS             48..98
                     /gene="hisL"
                     /locus_tag="ACHKUC_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO6620089.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    74..197
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            244..1143
                     /gene="hisG"
                     /locus_tag="ACHKUC_003915"
     CDS             244..1143
                     /gene="hisG"
                     /locus_tag="ACHKUC_003915"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO6620090.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1149..2453
                     /gene="hisD"
                     /locus_tag="ACHKUC_003916"
     CDS             1149..2453
                     /gene="hisD"
                     /locus_tag="ACHKUC_003916"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO6620091.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MACRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2450..3520
                     /gene="hisC"
                     /locus_tag="ACHKUC_003917"
     CDS             2450..3520
                     /gene="hisC"
                     /locus_tag="ACHKUC_003917"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO6620092.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTCEESQRVIDALRAEQV"
     gene            3520..4587
                     /gene="hisB"
                     /locus_tag="ACHKUC_003918"
     CDS             3520..4587
                     /gene="hisB"
                     /locus_tag="ACHKUC_003918"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO6620093.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQLFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4587..5177
                     /gene="hisH"
                     /locus_tag="ACHKUC_003919"
     CDS             4587..5177
                     /gene="hisH"
                     /locus_tag="ACHKUC_003919"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO6620094.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5177..5914
                     /gene="hisA"
                     /locus_tag="ACHKUC_003920"
     CDS             5177..5914
                     /gene="hisA"
                     /locus_tag="ACHKUC_003920"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO6620095.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEKDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5896..6672
                     /gene="hisF"
                     /locus_tag="ACHKUC_003921"
     CDS             5896..6672
                     /gene="hisF"
                     /locus_tag="ACHKUC_003921"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO6620096.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYNVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGTLAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6666..7277
                     /gene="hisIE"
                     /locus_tag="ACHKUC_003922"
     CDS             6666..7277
                     /gene="hisIE"
                     /locus_tag="ACHKUC_003922"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO6620097.1"
                     /translation="MLTERQRRELDWEKTDGLIPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTN
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(7517..>7688)
                     /locus_tag="ACHKUC_003923"
     CDS             complement(7517..>7688)
                     /locus_tag="ACHKUC_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO6620098.1"
                     /translation="KFKYRNREFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLS
                     IPYPGSPFTGRK"
BASE COUNT         1809 a         2063 c         2142 g         1674 t
ORIGIN      
        1 gtttaggtta aaagacatca gttgaataaa cattcacaga gatttttatg acacgcgttc
       61 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
      121 aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcatct
      181 tccgggggct tttttattgc gcagttgata acggttcaga caggtttaaa gaggaataag
      241 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat
      301 gactcacgcg aattactggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg
      361 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
      421 ggtctggtga tggacggcgt ggtagacctt gggattatcg gcgaaaacgt gctggaagaa
      481 gagctgctta accgccgcgc ccagggtgaa gatccgcgct actttaccct gcgtcgtctg
      541 gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg ggatggcccg
      601 ctctccttaa atggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
      661 gaccagaaag gtatctcttt taaatcctgc ttactgaacg gttctgtcga agttgcgccg
      721 cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagct
      781 aatggcctgc gcgaagtcga agttatctac cgctcgaaag cctgcctgat ccagcgcgat
      841 ggcgaaatgg aagaatccaa acagcaactg atcgacaaac tgctgacccg tattcagggt
      901 gttatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgcctggat
      961 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacta ttctaccgct ggcgggtgat
     1021 caacagcgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa
     1081 aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa gatgatggag
     1141 tgatcgctat gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac
     1201 gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc accgttaacg
     1261 atattctcga taacgtgaaa acgcgtggcg atgaggccct gcgtgaatac agcgcgaagt
     1321 ttgataaaac cacggttacc gcgctgaagg tgtctgctga ggagatcgcc gccgccagcg
     1381 aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct
     1441 tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc gtgcgttgcc
     1501 agcaggtcac gcgcccggtg gcttcagtgg gtttgtatat tcctggcggc tccgccccgc
     1561 tcttctcaac ggtattaatg ctggcgactc cggcgcgtat tgcgggctgt aaaaaagtgg
     1621 tgctgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag ctgtgcggtg
     1681 tgcaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg tttggtacgg
     1741 aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc accgaagcaa
     1801 aacgccaggt aagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg
     1861 aagtgctggt gattgctgac agcggcgcaa cgccggattt cgtggcttct gatttgctct
     1921 cccaggctga acacggcccg gactcacagg tgattttact gacgcccgac gccgatatgg
     1981 cgtgtcgcgt tgccgaggcc gtcgaacgcc aactggcaga actgccgcgt gccgaaaccg
     2041 cccgccaggc actgagcgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag
     2101 agatttccaa ccagtacggc ccggaacacc tgatcattca gacccgcaac gcccgcgaac
     2161 tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca ccggaatcgg
     2221 caggtgatta cgcctccggc actaaccacg ttctgccgac ttacggttac accgccacct
     2281 gttccagcct cgggctggcg gatttccaga agcgcatgac cgtgcaggaa ctgtcgaaag
     2341 aaggtttttc cgcgctggct tcgaccattg aaacactggc cgccgccgag cgcctgaccg
     2401 cccacaaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
     2461 gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcagcgcg
     2521 tcgtctgggc ggtaacggcg atgtctggct gaacgccaac gaatatccca ctgccgtgga
     2581 gtttcagctc actcagcaaa cgctcaaccg ctatccggaa tgccaaccga aagtggtgat
     2641 tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca gccgtggcgc
     2701 ggacgaaggt attgaactgc tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct
     2761 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg gcgtcgagtg
     2821 ccgcacagtg ccgacgctgg acaactggca actggactta cagggcattt ccgacaagct
     2881 ggacggcgta aaagtggtct atgtttgcag ccccaacaac cccaccgggc aactaatcaa
     2941 tccgcaggat tttcgcaccc tgctggagtt aacccgcggt aaggcgattg tggttgccga
     3001 tgaagcttat atcgagtttt gcccgcaggc atcgttagct ggctggctgg cggaatatcc
     3061 gcacctggcg attttgcgta ccctttcgaa agccttcgcg ttggcggggc ttcgttgcgg
     3121 atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg ccccctaccc
     3181 gctctcgacg ccggttgccg acattgccgc acaggcgtta agcccgcagg gaatcgtcgc
     3241 catgcgcgaa cgggtggcgc aaattattac agaacgcgaa tacctgattg ccgcactgaa
     3301 agagatcccc tgcgtggagc aggttttcga ctctgaaaca aactacattc tggcgcgctt
     3361 taaagcctcc agtgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
     3421 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaacct gtgaagaaag
     3481 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
     3541 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt
     3601 gacaaactcg cctttgaacc gggcgtgatc ccggagctgc tgaagctgca aaaagcgggc
     3661 tataagctgg tgatgatcac caatcaggat ggtctgggaa cacaaagttt cccgcaggcg
     3721 gatttcgatg gcccgcacaa cctgatgatg cagctcttca cctcgcaagg cgtgcagttt
     3781 gatgaagtgc tgatttgccc acacctgccc gccgatgagt gcgactgccg taagccgaaa
     3841 gtaaaactgg tggagcgtta tctggctgag caagcgatgg atcgcgctaa cagttatgtg
     3901 attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa tggtttacgc
     3961 tacgaccgcg aagccctgaa ctggccaatg attggcgagc aactcactaa acgagaccgt
     4021 tacgcccatg tagtgcgcaa caccaaagag acgcaaattg acgtccaggt gtggctggat
     4081 cgcgaaggtg gcagcaagat taacaccggc gttggcttct ttgatcacat gctggatcag
     4141 atcgctaccc acggcggttt ccgtatggaa atcaacgtca aaggcgacct ctatatcgac
     4201 gatcaccaca ccgtcgaaga taccggcctg gcgctggggg aagcattaaa aattgctctt
     4261 ggcgacaaac gcggtatttg ccgctttggt tttgtgctgc cgatggacga atgccttgcc
     4321 cgctgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagctga atttacctac
     4381 cagcgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc gctttcatac
     4441 accatgggcg tgacgctaca cctgaaaacc aaaggtaaaa acgatcacca ccgtgtagag
     4501 agcctgttca aagcgtttgg ccgcaccctg cgccaggcca tccgcgtgga aggcgacacc
     4561 ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatact ggctgcgcca
     4621 acctgaactc ggtgaagtct gccattgcgc gccacggtta tgaacccaaa gtcagccgtg
     4681 acccggacgt cgtgttgctg gccgataaac tgtttttacc cggcgttggc accgcgcaag
     4741 cggcgatgga ccaggtgcgt gagcgcgagt tgtttgatct catcaaagcc tgtacccaac
     4801 cggtgctggg catctgctta gggatgcaac tgctggggcg gcgcagcgaa gagagcaacg
     4861 gcgtcgattt gctgggcatc atcgacgaag acgtgccgaa aatgaccgac tttggtctgc
     4921 ccctgccgca tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg
     4981 ggattgaaga cggtgcgtac ttttactttg ttcacagcta cgcaatgcca gtcaatccgt
     5041 ggaccatcgc ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact
     5101 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa
     5161 acttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
     5221 tctccatcag ggcgactacg gcaaacaacg cgattacggt aacgacccgc tgccgcgctt
     5281 acaggattac gctgcgcaag gtgccgaagt gttgcacctg gtggatctga ccggggcaaa
     5341 agatccggct aagcgtcaaa tcccgctgat taaaaccctg gtcgcgggcg ttaacgtccc
     5401 ggtgcaggtt ggcggcggtg tgcgtagtga aaaagacgta gcggcgttac tggaagcggg
     5461 cgttgcgcgt gtggtggtcg gctccaccgc ggtgaaatca ccagaaaggg tgaaaggttg
     5521 gtttgaacgt ttcggtgcgg atgccttagt gctggcgctg gatgtccgta ttgacgagca
     5581 aggcaacaag caggtggcgg tcagcggctg gcaagagaac tcgggcgttt cactggaaca
     5641 actggtggaa acctatcttc ccgtcggcct gaaacatgtg ctatgtaccg atatctcgcg
     5701 cgacggcacg ctggcgggat ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc
     5761 acaggtggca tttcagtcct ccggcggtat tggcgacatt aacgatgtgg cggccctgcg
     5821 tggtactggt gtacgcggcg taatagttgg tcgtgcatta ctggaaggta aattcaccgt
     5881 gaaggaggcc atcgcatgct ggcaaaacgc ataatccctt gtctcgacgt tcgtgatggt
     5941 caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtgccactg
     6001 gcaaaacgct acgccgaaga aggtgcagat gaactggtgt tctacgatat caccgcttcc
     6061 agcgatggcc gtgtggtaga caaaagctgg gtatctcgcg tggcggaagt gatcgacatt
     6121 ccgttttgtg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt
     6181 ggcgcggata aaatctccat taactcccct gcgctggcgg acccgacgtt aattactcgc
     6241 ctggcggatc gctttggggt gcagtgtatt gtggtcggca ttgatacctg gtacgacgcc
     6301 gaaaccggta aatataatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact
     6361 cagtgggaaa cactcgattg ggtgcaggaa gtgcaaaaac gcggtgccgg agaaatcgtc
     6421 ctcaacatga tgaatcagga cggcgtgcgt aacggctacg acctcgaaca actgaaaaaa
     6481 gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg gcgcaggcac catggaacac
     6541 ttcctcgaag ccttccgcga tgccgacgtt gacggtacgt tggcagcttc cgtattccac
     6601 aaacaaataa tcaatattgg cgaattaaaa gcgtacctgg caacacaggg cgtggagatc
     6661 aggatatgtt aacagaacga caacgtcgcg aactggactg ggaaaaaacc gacggtctga
     6721 taccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tacatgaacc
     6781 cggaagcctt agacaaaacc atcgaaagcg gtaaagttac cttcttctcg cgcactaaac
     6841 agcgactgtg gaccaaaggc gaaacctcgg gcaatttcct caatgtggtg agcattgcgc
     6901 cggactgcga caacgacacg ttactggtgc tggcgaatcc catcgggcca acctgccaca
     6961 aaggcaccaa cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg
     7021 aacaactgct cgccgaacgt aaatctgccg acccggaaac ctcctacacc gccaaactgt
     7081 atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac
     7141 tggcagcaac ggtacatgac cgctttgaac tgaccaacga ggcgtctgat ttgatgtatc
     7201 acctgctggt gttgttgcag gatcaggatc tggatttaac aacggtgatt gagaacctgc
     7261 gtaaacggca tcagtgagtg gcgtgctgac cggatgcgat gttgttgtcg catccggagc
     7321 cattgcccga tgcgcaagct tatcgtgcct acacattatc tcaacacacg taggccggat
     7381 aagatgcgcc agcatcgcat ccggcaaaat aaatatcctc cggcatagcc ggaggttttt
     7441 caaatgcgcc tataaggctc tcttaccagc cgcgccctaa caggcgcaca cgatctgaca
     7501 tttgcatcca acttcgttac ttacggcccg taaacgggct gcccggatag ggaatcgata
     7561 actgctctcc cattttatcc tcttcaagct ggtgctttat gtaatcctgt atcttcgccg
     7621 tgttcttacc caccgtatcg acgtaatacc ctctgcacca gaactccctg ttcctgtatt
     7681 tgaatttc
//
LOCUS       DAZFRG010000218         7637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFRG010000218 DAZFRG010000000
VERSION     DAZFRG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.219"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..186
                     /gene="rclB"
                     /locus_tag="ACHKUC_003924"
     CDS             <1..186
                     /gene="rclB"
                     /locus_tag="ACHKUC_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO6620099.1"
                     /translation="FSTNADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKV
                     TSMRIDNNSTATAVLYK"
     gene            198..791
                     /gene="rclC"
                     /locus_tag="ACHKUC_003925"
     CDS             198..791
                     /gene="rclC"
                     /locus_tag="ACHKUC_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO6620100.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(951..1820)
                     /locus_tag="ACHKUC_003926"
     CDS             complement(951..1820)
                     /locus_tag="ACHKUC_003926"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6620101.1"
                     /translation="MEFSVLSNNLKMPMMGFGFFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            2068..2925
                     /locus_tag="ACHKUC_003927"
     CDS             2068..2925
                     /locus_tag="ACHKUC_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620102.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(3050..7300)
                     /gene="fdeC"
                     /locus_tag="ACHKUC_003928"
     CDS             complement(3050..7300)
                     /gene="fdeC"
                     /locus_tag="ACHKUC_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO6620103.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMTTA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKTSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGGYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTSLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADNTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         1894 a         1941 c         1694 g         2108 t
ORIGIN      
        1 ttttccacca atgcagatga taaaataatt ctgaaacata tcagcgtctc atcagtatca
       61 gcatcaccga cagttcttga gaatgccatt gctgatatgg ccagaaaata taatgcttca
      121 tcctggaaag ttacatcgat gcgaattgat aataattcaa ccgcaacagc agtattgtat
      181 aaataaggat gtttacaatg gaaagatacc tgcacctgtt aagtcgggga gataaaattg
      241 gcctgacatt gattcgtctg agtattgcga ttgtttttat gtggattggg ttattaaagt
      301 ttgtccctta cgaggcagac agcattacac cattcgtcgc aaacagtcca ctaatgtcgt
      361 tcttttatga acacccggaa gactataaac agcatctgac tcacgaaggc gaatacaaac
      421 cagaagcaag agcatggcaa acggctaata atacctatgg tttttccaac ggtcttggcg
      481 tcgtggaggt gattattgct ctgctggttt tggctaatcc tgtcaatcgc tggttaggtt
      541 tattgggtgg gctgatggca tttaccacgc cgctggtaac actctcattt ttaatcacca
      601 ccccggaggc atgggtaccc gcattgggtg acgctcatca tggtttccct tatttatccg
      661 gtgctggtcg tctggtattg aaagatactc tgatgctggc aggtgctgtc atgataatgg
      721 cagattcggc gcgggatatt cttaaacaac gcagtaatga atccagttca acgttaaaaa
      781 ctgaatattg ataatccgaa cactgtctgt tgttcacttt ttcaggcgtc aggctccctt
      841 tctgatgatg aagcctgacg ccatattacc ggagatatga cagagtgaag gtcaatatca
      901 ctcccctatt aaaagattct tgtcgtctca ggtaaaataa gtgattaata tcaatcatgt
      961 atttttacag caagacaacc acgaacaaac tcaggattaa aatgttttac cgattcccca
     1021 acataaccca aatcaagcga actaatttgc gccatttctt tctctgtcag tgagaaatcc
     1081 caaatagcaa aattttcttc gatacgctgt tgatgtgtcg atttcggaat aacgaccact
     1141 ccccgttgaa tattccaacg cagaatgacc tgagaaatcg atttttgatg ggcatctgca
     1201 atactctgaa gcagattatt ttcaaagggt ttatgtcgtc caccacctaa cggagcccat
     1261 gcttcaggct gcacgttata atacttcatg gttgccagcg cctctggttg ggcaaaatag
     1321 ggatgcaact cgacctggtt gaccatcggt ttaactctta ccgtttcaca aaagttcgcc
     1381 agaacatgag ggtagaaatt ggaaacccca atcgctttta atttgccctc ttcataggca
     1441 tcttcgagtg cacgccacgc actaaaataa tcgcgcatgg cctggtgcaa taaatagaga
     1501 tcgaagtatt ctagccccga tttttttaat gatgcttcaa tacctgctgc tgcaatatcc
     1561 tgattcaaca tatcctgcac ccacaattta gacgttataa ataactcttc tcgggtacat
     1621 aaaccttcag atatggcttc acgaacggct tcaccgacgg catcttcatt accgtatacc
     1681 gctgcagtgt cgatgagtcg ataacccgtg cggatagcat tcaacactga ctgtttgcac
     1741 acgtctttat cggtaacctg aaaaaaacca aatcccatca tcggcatttt cagattgtta
     1801 cttaatacgg aaaattccac tgtcagtccc tccattaggt atgaacaatg agtctacgtt
     1861 aaaacgtaac ctcaaattag tatgtggatt ttgatatcac ttatgcaaaa aattcattaa
     1921 taatgtagga ctgaaacctc tctatttttg gggcaatgaa gcagacgcta tcagtgcttt
     1981 tgccttggga cttgctgttt ttgatacact tagggcccag gacataacga aaatgtgcga
     2041 tatgacaaag aattaacgga gtatgtgatg atcaggcaga agattctaca gcagctcctg
     2101 gagtggattg agtgcaatct tgagcaccct atttcaatcg aagatatcgc acagaaatct
     2161 ggctacagca gacgcaacat ccagcttctg ttccgaaatt tcatgcatgt gcctttggga
     2221 gaatacattc gcaaacgaag gctttgtcgt gccgccattc ttgtccggct ctccgcgaaa
     2281 tctatgcttg atattgcact ctctttgcat tttgattcac agcagtcatt cagccgcgaa
     2341 tttaaaaagt tattcggctg ctctccccgt gaataccgcc accgtgatta ttgggatctc
     2401 gcaaatatct tcccttcttt tttaatacgt caacagcaaa agacggagtg tagattagtc
     2461 aactttcctg agacacctat ttttggcaat tcatttaaat atgatattga agtgtcgaat
     2521 aaattaccgg atgaagaagt caaactgcga cgtcatcatt tagtcagatg tatgaagaat
     2581 tttaagacgg atatctattt cgtctccacg ttcgaaccat caacaaaatc ggtcgatttg
     2641 ctcacagttg aaacttttgc cggtacggta tgcaaacaaa ccgattcaga gatgccgaaa
     2701 gagtggacaa ttaaccgagg actgtatgcc tctttccgct atgaaggtga gtgggagcac
     2761 taccctgaat gggcgcgaaa cctctatctg atggaattac ctgccagggg gttagccaga
     2821 gtgaacggca gtgatattga gcgattttat tacaatgaaa acttcgtcga aattgatagc
     2881 aataacattg tttgcgaaat ttttatcccc gttcgcccgg tttagcctgt aactccatca
     2941 cataagattt accctaccct gccaccgtct aatcggtgcc agggtaggta ttcagtaaat
     3001 aatggcgctg cgcatgtcaa cgccattatt atttattaca tcgtcgtttt tatttctcct
     3061 cagcgccttc agtatctgca ggaacggcgt taattgtcac agtcactggt ttcatttcat
     3121 accctccata tgtcaaagag ttgaatgtga atgtgtatgt acctggcttg ttggtactaa
     3181 atgtacgcgt gattttcccg ctcgcgaaat ggtcattttt cgacggcagg aactgataat
     3241 ccgcctctgt caccccttcc ggagcctcaa tgtcgaccca catggttcct cctacggggt
     3301 tgacacccga catcagagta accgtcagtg ttgcagtagt cttcccgtcc gccaccaaag
     3361 aatctacacg ctctggagat gcagcgagca ccgcgttttt atcatccgcg acgaatttag
     3421 attcggcagt tttcgtcgat tcctgaccgt cggcctgttc cactttcgcc gtgagtgtat
     3481 atgtcgctgc gacagtggtc gtggcggtga aaatagcctg cccttgctca ttcgttgtcg
     3541 ccgtcccatt gggagtcaga actaaatttt ccgggctggc agtcagcgtc acttcgctat
     3601 ctttcagcag gttgttattg gcatcagtca ccgtaacttt atacgtctgt ttggttttgc
     3661 cgtccgccac tgcacggttg ccaatcacct caatactgga gatggtcgcc gtagtgttat
     3721 cggccacaaa cgttacagac tgcgcattcg aaggctggtc atttcctgct gacgccgtga
     3781 tctcataggt tcctgcagtc acggaaacca ctttcagttc cgctttaccc tccttgtcgg
     3841 cgttcaccat gatattaccg tctgcaagcg gtttgacgcc ccgaggcaga ttaaagacga
     3901 ccacagcgcc aggaagaagg ttgccgtatt gatccttaac cgttgccgtc agcgtaaagg
     3961 catctttgcc gtttgccact ttttgagcag cggcgtctac ctccaggctc gcctgaccgg
     4021 tactgaaatc cgccttgaat ttcaccttga ccgtcttctg agagtttttc accgaggcct
     4081 cgatctcaag ttcccctgca accgttgaca taagctcaat gtccactttc cctgccgcat
     4141 tggttgttac tgtattcccc gtcttgctgg tcacaccctg cggtaaagtc agcgtaactt
     4201 cttgcccctg caacgggtta ccatagctgt ccacgacggt cagggttatc tggttagcag
     4261 actgtccatt agccagttgg ttattaacct tcactgtcat atcacgaatc tcagccttag
     4321 atgcgtcacc tgcaacattc agcaccagtg gctgagcaac ggcattttgg ccgttcactc
     4381 gcggcatcac agacaactga cccgcggcag atcccagcgt taaggtcgac acgtagaccc
     4441 cattaccttt ctctgtccag tttcctgctg aaggacgctc actccccgtg ctggcggcac
     4501 cactaaacac tggtgcatct ggcttcagcc cggtgaccgg attcccgtac gcatccttca
     4561 cggtgaaggt gagttccgtc gtcgttttaa ccgttggagt tttattgtcc gcaacaagcg
     4621 tagagttcgc tgatgacatc tctccggcaa taacagtcag ctgcgcggct tcggtggctg
     4681 caggctggcc gttgaagagc ggcatgacac gaagctcgcc cgtctttccg cctgtagtta
     4741 acgtagcaac ataggaaccg tcaccttttt cggtccaact ggaaacggtc gccccttcag
     4801 aggcggtccc cgtcaaactt gccgacaacg aaagaccact gatagcgttg ccatgcgcat
     4861 ctttcgccac cagcgttacg gttgtgcttt caccggcttt tacgtgatct tctgcgacag
     4921 agactttcga ctggtttgaa gataatgcat cagccaccac ggttactttt gccgcatttg
     4981 ctgccgcgtc ctgcccattg agcttcggca taacgtctaa ttcacccgcc gtagtgccga
     5041 gagaaatctg cgcagtccag gtcccgtcgc cattatccgt ccagcctgat gccgtagaac
     5101 cagcagcagc tgcgcccgat aatgacggtg catccggatt gaggctggtc acagggttgt
     5161 cattagcatc ttttgccgtc cagattgccg taactgtacc gccaaccacc ggtttatcag
     5221 gattcagtgt gatggacgaa tgtgctgcat caagcggccc ggcaacaaac ttcagcgttt
     5281 gttgtaatgt gcccagctga tactgctcgc tgaaggcacg cacaacaacg tttccggcac
     5341 gggtcgaaga aaccgtggcg ctgtaacccc ctggttttat ttccgaaatg gcaccaacgg
     5401 ttacaccatt agtgtcttgc ggaacaagtc gcaggcggct ggcttctccc gtcaccggat
     5461 taccctcgga gtccaccgct gtcagtgtca gcgtgtaggc ttgctgacca tcagcaacca
     5521 catcacctga cggctcgtta gcgctcaggg tggagtttgc cacatccatc atcgtggccc
     5581 gcagttctgc agtgacagtt ttgctcatgt catcaacgct aacagtaatc gttgcctcac
     5641 ctgactgcgt tccggtagta aagacagact gatacacccc tgcttcagtt tcggtgaact
     5701 cacccagtgt tggctttgcc tgtgatttag tggccttcag ggtacgagtc acaatatttc
     5761 cagccggttt gaaggttagt tcagtcttga tctgatcttt catgcccgtg actggctggc
     5821 cctcggcgtc gcgcagagac agcaccagcg gcttttgctc attaccgtta gcaagcattt
     5881 gaatacggct ctgaccgtca agcgttaacg ccgtacgatc ggcgctcata ccagctccgc
     5941 taataaccac ttctgtctgc acacgtttcg aggcattgcc tttgttatcg taggcaatcg
     6001 ctgaaatcgc ataataattg tctttgcctg cctgataagc cgggagcgtc acttgccact
     6061 gattgccctg ccccgtaatt tttccgcctg cggccagcaa agacggcgct tcccattgca
     6121 catttttcag accgtgagtt gctttgctga ccacaagccc cagggaaacc gtctggccgc
     6181 ccttgccttc aatacgctca ggcagagcaa tacggatcac ttcagatttg cgatactcaa
     6241 gaacgatatt gttattacgc tcaaccaggt catagcggct gcctgccagc attcgacgct
     6301 cgcgaatgct gtctgtatcg agttgttttt ccagaggttc gccaatccga taattaactt
     6361 ccaggccaaa gcgagtgtca ttctcaccac tcttgccctg cttatgcccg gcactcaggg
     6421 tcagaagagg cactggcgtg taattcactt cagcggtaat cgcatgtgga tctttctggc
     6481 gtttatcttt accaaacagc ccgacttcat cgccataata ctgttcatac atcaggcttg
     6541 cgccaagctg cggccaggca ggtaaatagc cctcagcacg aatatcccag ccattcgccg
     6601 ggcgttcctg ataatcctca acatccggcg atgttttcca gccagaagcc cggatataac
     6661 cattggcgct cagtttcaaa taatcacgcc agtattccgc accaacacca atgcgggtat
     6721 gactacggga taaatcatga tcgataaaag tattcacccc cgccatccag tcattttctg
     6781 aaaaatgacg ccagccaaaa ccaatatttg actgagtacg atcgtcggta cgatgtattg
     6841 ccccctgagt gaacaacata tttgtcggtg tatcataaat cggataaagc atttccagcg
     6901 aagagtcctt cagcgagaaa tttttatcga cattcagttt gacgcgcgca gtaccgtatt
     6961 tcccgagcca ctcctgaatt tcctggttag ctttagcggt ggtcattccg gtaataaagt
     7021 tacgtgtcgc atcgctatct ggctgactgc ttaaaaatgt cccggcatta gcggcaagcg
     7081 acgcgacatt tttctccacg ttattatcag cagttaccgt agtattttcc atgctcaacc
     7141 gtggctgaac cgcatgctgc gcacgtgctg ccattactgg cgtaaaggtg acagcgagtg
     7201 gaaaaagaac ctgaacagag atatttgccc acgccacgca gcgggccaga actgaataac
     7261 gaaatcgtgg ctgtttattg tctgttttat aacgtgacat tgagataatt tcctgatgaa
     7321 cgaattaacg aacataggca gacacaaata atggcgtctg cttgtcagaa aaaccgggga
     7381 tttcccggtt tccttttcca cagaaaagga aataccttta tgtaagaaag tacaccgcca
     7441 cctaatatat ccagtgtctg tagccactca tcaggtggtt gggcgcggga ttatattttt
     7501 atttatgggt aaaaatttag ctttttgtgc aagtcattaa atagatactt aagttatatt
     7561 tcttactctg cgggcagtga ataattcatt attcagagtg aataataatg acccgtcttt
     7621 aattaactgc tattggg
//
LOCUS       DAZFRG010000219         7619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFRG010000219 DAZFRG010000000
VERSION     DAZFRG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.220"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..171
                     /locus_tag="ACHKUC_003929"
     CDS             <1..171
                     /locus_tag="ACHKUC_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO6620104.1"
                     /translation="ASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYF
                     STYMQEELKALV"
     gene            complement(291..470)
                     /gene="yoaH"
                     /locus_tag="ACHKUC_003930"
     CDS             complement(291..470)
                     /gene="yoaH"
                     /locus_tag="ACHKUC_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO6620105.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            544..1905
                     /gene="pabB"
                     /locus_tag="ACHKUC_003931"
     CDS             544..1905
                     /gene="pabB"
                     /locus_tag="ACHKUC_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO6620106.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1909..2487
                     /gene="nudL"
                     /locus_tag="ACHKUC_003932"
     CDS             1909..2487
                     /gene="nudL"
                     /locus_tag="ACHKUC_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO6620107.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2671..4035
                     /gene="sdaA"
                     /locus_tag="ACHKUC_003933"
     CDS             2671..4035
                     /gene="sdaA"
                     /locus_tag="ACHKUC_003933"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6620108.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4166..5764
                     /gene="pdeD"
                     /locus_tag="ACHKUC_003934"
     CDS             4166..5764
                     /gene="pdeD"
                     /locus_tag="ACHKUC_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6620109.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5768..7324)
                     /gene="yoaE"
                     /locus_tag="ACHKUC_003935"
     CDS             complement(5768..7324)
                     /gene="yoaE"
                     /locus_tag="ACHKUC_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO6620110.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7312..7470)
                     /gene="yoaL"
                     /locus_tag="ACHKUC_003936"
     CDS             complement(7312..7470)
                     /gene="yoaL"
                     /locus_tag="ACHKUC_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO6620111.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
BASE COUNT         1855 a         2001 c         1925 g         1838 t
ORIGIN      
        1 gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc tggtttcacg
       61 gaaaaaatgg ttggatgggc aaaaaaaatg gagtctggtg aacgctctgt gattaaaaat
      121 cctgaatact tttcaacata tatgcaggaa gaactaaaag cactggtttg agttaaattt
      181 acatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc ctttttctca
      241 caatcaaata atcatcggct tctataacga aagcgatacg gattagtgtg ttactcgtct
      301 tcatcctcaa aacgcgccac gattcgctcg ccggaatggt tggcacgcag ctcttctgcc
      361 accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg gatccgctcg
      421 acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat tgttaactcc
      481 tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt agagtcagga
      541 ctgatgaaga cgttatctcc cgctgtgatt actttaccct ggcgtcagga cgccgctgaa
      601 ttttatttct cacgcttaag ccacctgccg tgggcgatgc ttttacactc cggctatgcc
      661 gatcatccat atagccgctt tgatattgtg gtcgccgagc cgatttgcac tttaaccact
      721 ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac tgatgacccg
      781 ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccagcgca taacgaagat
      841 ttgccatttc agggcggcgc gctggggttg tttggctacg atctgggccg ccgttttgag
      901 tcactgccag aaattgcgca gcaagatatc gttctgccgg atatggcagt gggtatctac
      961 gactgggcgc tggttgtcga ccaccagcgt caaacagttt ctttgctgag tcataatgat
     1021 gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga agatttcacg
     1081 ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa atttcgccag
     1141 gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca gcgttttcat
     1201 gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc caaccgcgcg
     1261 ccgtttagcg cttttttacg ccttgagcag ggcgcgattt taagcctttc accagagcgg
     1321 tttatccttt gtgataatag tgaaatccag acccgcccga ttaaaggcac gctaccacgc
     1381 ctgcccgatc ctcaggaaga tagcaaacaa gcagagaaac tggcgaactc agcgaaagat
     1441 cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg tgttgccgta
     1501 gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc cgtgcatcat
     1561 ctggtcagca ccataacggc gcaactacca gaacagttac acgccagcga tctgctgcgc
     1621 gcggcttttc ctggtggctc aataaccggg gctccgaaag tacgggctat ggaaattatc
     1681 gacgaactgg aaccgcagcg acgcaatgcc tggtgcggca gcattggcta tttgagcttt
     1741 tgcggcaaca tggacaccag cattactatc cgcacgctga ctaccattaa cggacaaatt
     1801 tactgctctg cgggcggtgg aattgttgcc gatagccagg aagaagcgga atatcaggaa
     1861 acttttgata aagttaataa gatattacgc caactggaga agtaagacgt ggaataccgt
     1921 agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca aattaaccgg
     1981 gaaaccttaa atcatcgtca ggctgccgtg ttaatcccca tcgtccgccg accgcaaccg
     2041 gggttgttgc tgactcagcg ttcgattcat ctgcgtaaac acgctggaca agtggcattc
     2101 cctggaggcg cagtcgatga cacggacgca tcagttatcg ccgctgcgct gcgcgaagct
     2161 gaagaagagg tcgctatacc gccttccgcc gttgaggtta tcggcgtgct gccgcccgtc
     2221 gatagcgtca ctggctatca ggtaacccca gtggtcggca ttatcccgcc cgatctgccg
     2281 tatcgcgcca gtgaagatga agtctccgcc gtctttgaaa tgccgctcgc ccaggcatta
     2341 catctgggtc gttatcatcc tttagatatc taccgccgtg gcgattcaca tcgggtatgg
     2401 ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat tcgtgagctg
     2461 gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca aaacactact
     2521 tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa ttcatgtgaa
     2581 tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt tcgttactgg
     2641 aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat gtttaaggtg
     2701 gggattggtc cctcatcttc ccataccgta gggcctatga aggcgggtaa acagttcgtc
     2761 gatgatctgg tcgaaaaagg cttactggat agcgttactc gcgttgccgt ggacgtttat
     2821 ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat tatgggtctt
     2881 gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat tcgcgacgta
     2941 gaagagcgcg aacgtctgct gctggcacag ggacggcatg aagtggattt cccgcgcgac
     3001 aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat gcaaatccac
     3061 gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg cggcggtttt
     3121 atcgtcgatg aagaacactt tggtcaggat gccgccaacg aagtgagcgt gccgtatccg
     3181 ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc gctgtctggt
     3241 ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga gtatttcgct
     3301 catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga aggcgtactg
     3361 ccaggcccgc tgcgcgtgcc gcgtcgtgcg tctgccctgc gccggatgct ggtttccagc
     3421 gataaactgt ctaacgatcc aatgaatgtc attgactggg taaacatgtt tgcgctggca
     3481 gttaacgaag agaacgccgc cggtggtcgc gtggtaactg cgccaaccaa cggtgcttgt
     3541 ggcattgttc cggcagtgct ggcttactat gaccacttta ttgagtccgt cagcccggac
     3601 atctataccc gctactttat ggcagcgggt gcgattggtg cactgtataa aatgaacgcc
     3661 tctatttccg gtgcggaagt cggttgccag ggcgaagtgg gtgttgcctg ttcaatggct
     3721 gctgcgggcc ttgccgagct gttgggcggt agcccggaac aggtttgtgt ggcggcggaa
     3781 attggcatgg agcacaacct cggtctgacc tgcgacccgg ttgcagggca ggttcaggtg
     3841 ccgtgcattg agcgtaatgc tattgcctct gtgaaagcga tcaatgctgc acgcatggcc
     3901 ttacgccgca ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac gatgtacgaa
     3961 accggtaagg acatgaacgc taaataccgc gaaacctcac gcggtggtct ggcaatcaaa
     4021 gtccagtgtg actaatactt cttactcgct catctgcaac ggatgggcga atttatcccc
     4081 gctttctcgt ctgctgtaat tttccccact acacttccac tgttgcgtca ggcgtttgtc
     4141 gccatacgct tacagggtgg cccgcatgca aaaagcacaa cggatcatta aaacctatcg
     4201 ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt cgaccctgag
     4261 cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat tcgccaatca
     4321 cgctgtagag gaattagata aagtactgct tcccctacag gcaggtagcg aagtcttact
     4381 tccgctaatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac aggcggcaaa
     4441 actccaaact gtgcgatcca ttggcctggt gcaagacggc acactttatt gctccagcat
     4501 ttttggctat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg caccgcaacc
     4561 acttttacga ctgacgaccg accgtgccct gataaaaggc agtccggttt tgattcaatg
     4621 gacgcctgca gcgggcagta gcaatgctgg ggtcatggag atgattaaca tcgacttact
     4681 gacggcaatg ctccttgaac cacagctgcc gcaaatcagt agcgccagcc tgacggtgga
     4741 cagtcggcat ttgctctatg ggaatgggct ggtagattcc cttccgcaac ctgaaaacaa
     4801 tgaaaactat caggtttctt cgcaacgctt tccttttacc attaacgtta atggcccggg
     4861 ggctacggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg tgctgctaag
     4921 tttactggtg ggctacatcg cctggctggc gaccgcttac cgaatgagct tttcccgcga
     4981 gatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc
     5041 acgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc cgcgtcaggg
     5101 ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa ttgtgccact
     5161 gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccaa tgagtagtca
     5221 gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga
     5281 tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg aaatcaccga
     5341 acgcgatgcc ttactggatg ttgattaccg gattgcccgc gagctgcatc gtaaaaacgt
     5401 caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc ttgaaacatt
     5461 acgtcctgac gttctgaaaa ttgataagtc atttaccgca gctataggtt ctgacgcggt
     5521 taactcgacg gtgaccgata tcatcatcgc actggggcaa agactgaata ttgaactggt
     5581 ggcggagggc gtagaaacgc aagaacaggc gaagtatttg cgcagtcatg gcgtgcatat
     5641 tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca aatggctggc
     5701 gggcagccaa ccgccgcccg cccggcataa tggacatatc acacccatta tgccgttacg
     5761 ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga accagatcaa
     5821 cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca atcacatcgc
     5881 ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc gcgatatcat
     5941 cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa tctgtaccgc
     6001 cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca gcatccggga
     6061 attcacccgc aatggcttcc agcacatcca gcggcgtgac caagccttgt accacaccaa
     6121 actcgttggt cacgataaca aagctcccgc gagcacgacg cagcacgccc agcaggttga
     6181 tcggatcgag ggtttcaggg acgataatcg ccggagacgc cgaagcaatc gccgccacat
     6241 caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgatacca atgatttcat
     6301 ccagttcacc gcgacatacc gggaacagac tgtgcggtga agagagcagt tgctcgcgga
     6361 tttcatcgac cccgagatta gcgtcaaccc agcttatttc accgcgcggc gtcatgatcc
     6421 cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt tcttcttcgg
     6481 caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc tgaacattgg
     6541 cctgacgttt cccgcccatc aaacggagaa tcgcatcggc agtacgcgct cgcagcggca
     6601 aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact tcgatgatga
     6661 tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg aaaccttctg
     6721 ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc accgtggggt
     6781 gctggttaac gaatcgcgtc agcggttttg atgccagcag cataaccgcc atcgcaatca
     6841 ctaccgccgc catcatcact ggcagatggt taaccatccc tactgccgta attaccgcat
     6901 ccaacgagaa gacggcgtca aggatgacga tctgtgtgac gaccacccag aaactggcgt
     6961 agcctttacc gtggccggaa tcatgatcgc ggttttccag ccgttcatgc agttcggttg
     7021 ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt ccggagaagg
     7081 aaaaatccat aacggtaaat agcggtttgg tcagcgtgac catccatgaa atcagcgaca
     7141 gcagccccag acgcataatc agcgccagtg ataatcccag caaacgcgct ttatcgcgtt
     7201 gttttggcgg cagtttgtca gcaagaatgg caatgaagac caggttatcg atacccagca
     7261 caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc attaagaatt
     7321 ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg caggcgtaac
     7381 gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt taaaaggcct
     7441 gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat actccatggt
     7501 tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat ttaccttcct
     7561 ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat cgaaagtta
//
LOCUS       DAZFRG010000220         7437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFRG010000220 DAZFRG010000000
VERSION     DAZFRG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.221"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..701
                     /locus_tag="ACHKUC_003937"
     CDS             <1..701
                     /locus_tag="ACHKUC_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620112.1"
                     /translation="NTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQVLDEG
                     EKFVGAAGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPYISDV
                     PDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAWKFHT
                     DNSLRGFRDKQAKLKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNAANAR
                     FVYQPFTRDQRKRG"
     gene            704..1471
                     /locus_tag="ACHKUC_003938"
     CDS             704..1471
                     /locus_tag="ACHKUC_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620113.1"
                     /translation="MQPSPAMQALIEQVYHIFRRYPVPQKFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVVQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYMLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQPEQGTQYQNWEDDWMIDECLCAMYDASSESPGK"
     gene            1474..2079
                     /locus_tag="ACHKUC_003939"
     CDS             1474..2079
                     /locus_tag="ACHKUC_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620114.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALSFSVMGGIGWQFSRRMPPELKNRLHCWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWCSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(2133..3638)
                     /gene="glpD"
                     /locus_tag="ACHKUC_003940"
     CDS             complement(2133..3638)
                     /gene="glpD"
                     /locus_tag="ACHKUC_003940"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO6620115.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARREKGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKTPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSQAS"
     gene            3828..4154
                     /gene="glpE"
                     /locus_tag="ACHKUC_003941"
     CDS             3828..4154
                     /gene="glpE"
                     /locus_tag="ACHKUC_003941"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO6620116.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAAQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            4199..5029
                     /gene="glpG"
                     /locus_tag="ACHKUC_003942"
     CDS             4199..5029
                     /gene="glpG"
                     /locus_tag="ACHKUC_003942"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO6620117.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            5046..5804
                     /locus_tag="ACHKUC_003943"
     CDS             5046..5804
                     /locus_tag="ACHKUC_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO6620118.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(5786..7384)
                     /gene="rtcR"
                     /locus_tag="ACHKUC_003944"
     CDS             complement(5786..7384)
                     /gene="rtcR"
                     /locus_tag="ACHKUC_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO6620119.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETKVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEEYLIHITTGTHVAQICWFLLAEARYLPTRLIQSSPPRKKERPDSPGAVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQES
                     RPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRELFDISRQGKASVNDA
                     DRLRKYLARFGLTWEAMQDQHSSS"
BASE COUNT         1718 a         1874 c         1937 g         1908 t
ORIGIN      
        1 ttaatacatt atttgcaagc ctcgctggta ctgatccttt cacaggcgtg gatattacga
       61 ttgccaactg caaatcggca tactgggatg aaggcattgt ccagcaactc attaatcagg
      121 tcctggatga aggggaaaaa ttcgtcggtg ctgccggtct tgagggattg cttcgatata
      181 atgtcacatt aaatattggc ttaacctcca gcaatgtatg gccgggattt tccctcgata
      241 ccgccacgat cggtcgtctt tgtgcctgtg gtgctgattt tggatttgac ccctatatca
      301 gtgatgtacc agatgttcaa tgtgacctga atacgacaaa tgactttacc gttcatttta
      361 cggcaatgct caatccggat gagcgggtga tcatagcaaa gcgcccgctt aaaaaatgtg
      421 actcttggat tgaggatgtt tatatctttc aggtgtttaa agaagcatgg aaattccata
      481 ccgataacag cttacgcgga tttcgcgata agcaggctaa actgaaactc tacgcgcgtc
      541 attactcagt ggaaaattgc acagaggaaa gctgtcggga ttgtaattat tgcattcggc
      601 ccagcttttc cctctctcgt agttccatta ttcgcctgaa tgcggcgaat gctcgttttg
      661 tttatcaacc ctttacacgc gaccagcgga agagggggta attatgcaac ccagccccgc
      721 catgcaggca ttaattgagc aggtttatca tatttttcgt cgttatccgg tgccgcagaa
      781 atttgtggtt tgttgtgaat attgcctgag tcagcaggaa caaaaggcat tacggaatac
      841 atcgttacga gcgatcccgt attccttaat caatgcctgg aatagtagtc caggacctga
      901 tccgcaaaac agcgatgagg ttcgttattt tctgcctcgt ttactggagt ttgttgtgca
      961 ggggcaattt gacaatattc acgaagtatt ttctctacgg cgtataaacc ttgccagtaa
     1021 agaaaactgg cgtgaggatg aacgggaaat attgcagcaa tttgcttgcc agtatatgac
     1081 tgattgggta tccggggatg aggctgtcga attacagtac atgctggaaa tgtttttccg
     1141 tgccgatatc gctctttcgc cattgcttga tgccattatt tcagttcccg gattttggtc
     1201 ggcagcgtct cttgcctgtt tattgaatac gtatcgtgac ggttatatca gagataacca
     1261 ggacgatatc gataaagcca ttaccactca aactaatact tgggctagta ataatcaatc
     1321 catcctgaaa gaacgcgcgc ggcaggcgat tgagaatccg cttaagcagc ccgaacaggg
     1381 cacacagtat cagaactggg aagacgactg gatgattgat gagtgtttat gcgcgatgta
     1441 tgacgcatca tccgaatcac caggaaaata aaaatgatta aattccggct ttatattccc
     1501 cctgtaattc tcggttttgt tatcgtacca ttattggtat ggccgacggt tattgcctta
     1561 gccgtactta tattcacgtt aacttttctg gcggaaataa tattctcctt tccgctcctg
     1621 gttgtgcgta tttctcttca ggaattacaa cttgagttaa tggttgaata tgcactttct
     1681 ttcagtgtaa tgggtggcat cggttggcaa ttctcccgca gaatgcctcc tgaattaaaa
     1741 aacagactgc attgctggct ggtcttttct ccggcctatt tctggttaat tctctcgaat
     1801 ttcattcttt atattactcc agagaaatca gcgttgctgg aaaatatccg aaacttcttt
     1861 ctgacgtttg tctggcttcc cctgaatttt tccccttttt ggccgcagcc gtggactgat
     1921 tttgtcggcc cgattagtgc acagcttggt tttgcgttgg gatattactg ccagtggtgt
     1981 agcaaaaata gaagccaggg gcagaagtgg gttgagtggg gaacgtgctt aagtttggcg
     2041 attttagctc aggggccgtt attcaattat ttacaatgaa tagtctaagg aattcaaata
     2101 gcccggttgc acttcacaac cgggcatcat ccttacgacg cctgcgacaa cctctgctgc
     2161 gtatattcca ccagccactg actcacacga gattgttgat ccgcatttag ccacatgcct
     2221 tgtttcgtgc gacgccacag ggcatcgtcg gcgcggcgga cccattcgtg atccaccaga
     2281 tatttcagct ccgcttcgta gaactcatga ccgaaatctt caccgaggtc gcttatcgcc
     2341 ccggcattgc cgagcagcag ttcgctgttg ctgccgtaag tgcgcgcgta gtgacgtgcc
     2401 agcgattcgg tcaggaacgg gtaacggcgg cgcaggcggg cggcgtaatc gtcacggtcg
     2461 ccttcaatgg caccacccgg cagcacgctc tctttcgtcc acgccggacc aatgccctga
     2521 taatatggcg tcagtttttc cagcgcgtgc tccgccagtt tgcggtaggt ggtcagctta
     2581 ccgccgaata ccgacagcag cggggttttg ccgttttcat catggatatc aagggtgtaa
     2641 tcacgggtaa tggcctgcgg cgagtcggac tcatcatcac atagcggacg cacgccggag
     2701 taggtccaga cgatatcgtc ccggcttaac tgctttttaa agtgcgtgtt atacactttc
     2761 aacaggtaat tgatttcact ctcttcaatc ttcactgctt ttggatcgcc tttgtattcc
     2821 acatcggtgg tgccgatgat ggaaaactcg tccatccacg ggatcacgaa gacaatacgt
     2881 ttatcttcgt tttgcagaat gtaggcttgc ttctgggtat gcacacgcgg caccacaata
     2941 tggctgcctt tgatcaggcg aatgccataa ggtgaaggca gatgcatccc gtcgtcgaaa
     3001 aattgtttca cccacgggcc ggtagcatta actaggccgc gcgcctgcca tgtatatttt
     3061 ttgccggtat cgatatcttc cgcttccaca atccacaggc ctttttcgcg gcgagcagac
     3121 gtggcacgag tacgagtaag cacttcaccg cctttacgca ccaccatctg ggcgttggcg
     3181 agtaccagac gggcgtcgtc tacccaacag tcagaatatt cgaatccgcg cttaatttcc
     3241 ggttttaaca ctgagtttgc gccaaaacgc aaaccggttg atcccggcag gctggtgcgc
     3301 ttacccagat gatcgtacat aaacagacca atgcgaatca tccacgccgg gcgcagatgc
     3361 ggacgatgcg gcaggcgaaa acgcatcggg aaggcgatat gcggggccat tttcagtagc
     3421 acttcacgtt cagccagcgc ctcgctgacc aggcggaatt catagtgctc aaggtagcgc
     3481 aggccaccgt gaatgagttt tgaactggcg gaagaggtcg cgcaagcgag atcctgcgcc
     3541 tccagcatca gcacggataa accgcgtcca gcggcgtctg ccgcgatacc agcaccattg
     3601 atgccgccac ctatcacaat cagatctttg gtttccatgc tgccctcatt cactttcgtt
     3661 aaagttcata aatgttcgtt atcgagcata ttagcaaaga atcgcgcttt gggtaacatt
     3721 gaaaaaacat tttagagtga tatgtgtaac attatggcgt ttatctgccg cttcgacgta
     3781 aactgtgcgg taaatttgcc cacttgtttg taaagaaaga gagacgcatg gatcagttcg
     3841 aatgtattaa cgttgccgac gcgcaccaga agttgcagga aaaagaggcg gtgctggtcg
     3901 atattcgcga tccacagagt ttcgcaatgg ggcatgcggc gcaggctttc catttaacca
     3961 acgacacatt gggcgcgttt atgcgtgata acgacttcga tacgccagtc atggtgatgt
     4021 gttaccacgg taatagcagc aaaggcgcgg cgcagtatct gctgcaacag ggctacgatg
     4081 tggtctacag cattgatggc ggctttgaag cctggcagcg ccagtttccc gcagaggtgg
     4141 cgtacggcgc gtaacgcttt atactgtccc cttttgtgtg gaataagcga cagcaacgat
     4201 gttgatgatt acctcttttg ctaacccccg agtggcgcag gcgtttgttg attacatggc
     4261 gacgcagggt gttatcctca cgattcaaca acataaccaa agcgatgtct ggctggcgga
     4321 tgagtcccag gccgagcgcg tgcgggcgga gctggcgcgt tttctcgaaa acccggcaga
     4381 tccgcgttat ctggcggcca gctggcaggc gggccatacc ggcagtggcc tgcattatcg
     4441 ccgttatcct ttctttgccg ccttgcgtga acgcgcaggt ccggtaacct gggtgatgat
     4501 gatcgcctgc gtggtggtgt ttatcgccat gcaaattctc ggcgatcaag aagtgatgtt
     4561 atggctggcc tggccattcg atccgacgct gaaatttgag ttctggcgtt acttcaccca
     4621 cgcgttaatg cacttctcgc tgatgcatat cctctttaac ctgctctggt ggtggtatct
     4681 cggcggtgcg gtggaaaaac gcctcggtag cggtaagcta attgtcatta cgcttatcag
     4741 cgccctgtta agcggctatg tgcagcaaaa attcagcggg ccgtggtttg gcgggctttc
     4801 tggcgtggtg tatgcgctga tgggctacgt ctggctacgt ggcgaacgcg atccgcaaag
     4861 tggcatttac ctgcaacgtg ggttaattat ctttgcgctg atctggattg tcgccggatg
     4921 gtttgatttg tttgggatgt caatggcgaa cggagcacac atcgccgggt tagccgtggg
     4981 tttagcgatg gcttttgttg attcgctcaa tgcgcgaaaa cgaaaataat tccagggatt
     5041 tagaaatgaa acaaacacaa cgtcacaacg gtattatcga actggttaaa cagcagggtt
     5101 atgtcagtac cgaggagctg gtggagcact tctccgtcag cccgcagact attcgccgcg
     5161 acctcaacga actggcagag caaaacctga tcctgcgcca tcatggcggt gcggcgctgc
     5221 cttccagttc ggttaacacg ccatggcacg atcgtaaagc cacccagacc gaagaaaaag
     5281 agcgcatcgc ccgcaaagtg gcggagcaaa ttcctaatgg ctcgacgctg tttatcgata
     5341 tcggcaccac cccggaagcg gtggcgcacg cgctgctcaa tcacagcaat ttgcgcattg
     5401 tcaccaacaa tcttaacgtc gctaacacgc tgatggtaaa agaggatttc cgtattattc
     5461 tcgccggtgg cgaattacgc agccgcgatg gcgggatcat tggcgaagcg acactcgatt
     5521 ttatctccca gttccgcctt gatttcggca ttctggggat cagcggcatt gacagtgacg
     5581 gctcgctgct ggagttcgat taccacgaag ttcgcaccaa acgcgccatt attgagaact
     5641 cgcgccacgt tatgctggtt gtcgatcact cgaaatttgg ccgtaacgcg atggtcaata
     5701 tgggcagtat cagcatggta gatgccgtct acaccgacgc cccgccgcca gtaagcgtga
     5761 tgcaggtgct gacggaccac catattcaac tggagctgtg ctgatcctgc attgcttccc
     5821 atgtcagacc aaaacgcgcc aggtatttgc gtagccgatc cgcgtcattg acgctggctt
     5881 taccctggcg cgaaatgtca aacagttcac gtccggcagc agaaagcgac tttgcctggc
     5941 ggcagatagc gataacgtgt tccagttgca tacggtcgaa gagatcgatg ttttctgcct
     6001 ctgcgcccag caacgccgta agcgtggagg ggcgactctc ctgccagtta tagcgcagac
     6061 ggttgatctc atcttccacc gtatccagtg tgatgcgtcc gctagtggca aaggtggcca
     6121 tccgcgtgac gctggcagaa agttcgcgaa agttaccgcg ccatgttgcc tggggagagg
     6181 tcgcaaaggc cagccaggca cgccgcgctt cggtgttaaa acgcacgctg tcgccagtga
     6241 gtgaggcgtg gcgctccact tcataatcca ggttcggttc aatatcttcc tggcgttggc
     6301 gtaatcctgg caaggtgaag gtccagagat ttattcgcgc gtacaggtct tcacgaaatt
     6361 tgccttcggc aaccaactgg cgcaaatcac gcaccgttcc ggcgataagc tgaaaatcac
     6421 tgctcacctg gcgatcgctg ccaaacgggt aaaaggtttt ctcttcaatg gctttcagca
     6481 gcattgcctg ttcgtctgcg cccagttcgc caatctcatc aagaaacaac attccgccgt
     6541 tggcgctgcg taataatcct tcacgcgatt cccgcgcccc ggtaaacgcg cctttcacat
     6601 gaccaaacag cgtcgacatg gcggtatcgc cgcgcagggt ggcgcagttc acttccacaa
     6661 acgcgccgct aaactgatgc cgcgcctgtt ttagctcgaa gatacgtcgt gccagaaatg
     6721 acttacctgc accggtcggg ccgttaagca ggatcggtgc gcgggatttt atcgccactt
     6781 tttcaatctg ctcaatcatg cggttgaagt gggagttacg cgtggcaata ccggacttaa
     6841 gaaaatcaag cgtttgctgg cgttcctcgg caaagcggct ggcgatggcg ttataacggc
     6901 ttaaatcgag atcgataatc gtcactgcgc cagggctatc gggccgctct tttttgcgcg
     6961 gtggtgaaga ttgtatcaac cgggtgggca ggtaacgcgc ttctgccagc agaaaccagc
     7021 aaatctgcgc gacgtgggtg ccggtggtga tgtgaattaa atactcttct ttatcaggtt
     7081 ggaacgcgta accacgggcg aaatcatgca ggcaggcgta aacttcttcg aaatcccacg
     7141 ggttatgcag ttcaatctca acgcccacca cttttgtttc tggcgaaacg ctggcgatat
     7201 cgcgtttaag cgtttcgaac agcgagcgcg agcgggcgtc gtgcaataat tccagccgat
     7261 cgataactaa cgattcttgc tggcatagac agagtgtcgg acgccatttt gaccagcgct
     7321 gactgccgcg cccggcataa tccagcaccg ttccgacaaa gccaaaagcc actgttttac
     7381 gcatcttaga tatccttata aaagacgaga tttcattatc ggtaaatcgg ctttgcg
//
LOCUS       DAZFRG010000221         7436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFRG010000221 DAZFRG010000000
VERSION     DAZFRG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.222"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="ACHKUC_003945"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="ACHKUC_003945"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO6620120.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAPVI
                     IAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQARE
                     FAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..2141
                     /gene="gatZ"
                     /locus_tag="ACHKUC_003946"
     CDS             879..2141
                     /gene="gatZ"
                     /locus_tag="ACHKUC_003946"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO6620121.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSRCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLGIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2151..2603
                     /gene="gatA"
                     /locus_tag="ACHKUC_003947"
     CDS             2151..2603
                     /gene="gatA"
                     /locus_tag="ACHKUC_003947"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO6620122.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALITRE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2634..2918
                     /gene="gatB"
                     /locus_tag="ACHKUC_003948"
     CDS             2634..2918
                     /gene="gatB"
                     /locus_tag="ACHKUC_003948"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO6620123.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGNIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2922..4277
                     /locus_tag="ACHKUC_003949"
     CDS             2922..4277
                     /locus_tag="ACHKUC_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6620124.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIMFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            4325..5365
                     /gene="gatD"
                     /locus_tag="ACHKUC_003950"
     CDS             4325..5365
                     /gene="gatD"
                     /locus_tag="ACHKUC_003950"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6620125.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSLEMSAP
                     QMQGVLRELRFNQLILEAAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5471..6244
                     /locus_tag="ACHKUC_003951"
     CDS             5471..6244
                     /locus_tag="ACHKUC_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6620126.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHKUC_003952"
     CDS             complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHKUC_003952"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO6620127.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMRLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASAWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1856 a         1672 c         1955 g         1953 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcgcagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccagcatgca
      121 tgcgccggtc atcatcgccg gaacgcccgg cacatttact catgccggta cagaaaatct
      181 gatggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcga ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgttc aggcgcgtga
      481 atttgccgag gcaaccggga ttgattccct ggcggtcgcc atcggcacgg ctcacggtat
      541 gtatgccaga gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 tttaccgctg gtgctgcatg gcgcgtcagg attatcgact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccccga agcgactgat ccccgtgatt atttgcagtc
      781 ggctaaatct gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatt ggcatatgtt cagtctgttc tgcacatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgtaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttgcgat tgccgataaa gtcggatttg ctcgtgagcg tattattctc ggcggcgacc
     1141 atctgggacc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa ggcatatgtt cgtgccggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgctga cgattccata ccgttagcgc cagaaacggt tgctgaacgt gccgctgtgc
     1321 tttgtctggc ggcggaaagt gtggcgactg attgccagcg tgagcaactg aactatgtca
     1381 ttggcactga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
     1441 cccaggttga agatgccgcc aatactttac gcacgcatca aaaggccttt attgcccgtg
     1501 gtctggcaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggt gtggaatttg
     1561 accatagcaa tattattcat tatcaggcgc aagaagctca ggcgttggcg caatggatag
     1621 aaaaaaccaa aatggtttat gaagcgcatt ccaccgatta ccagactcag acggcctatc
     1681 gggaattagt tcgcgatcat tttgcaattt tgaaagtcgg tccggcatta acctttgctt
     1741 tacgtgaggc gatatttgcg ctggcacaaa ttgagcagga acttatcgca cccgaaaatc
     1801 gtagccgttg tctggcggta attgaagaag tgatgctcga cgaaccgcaa tactggaaaa
     1861 aatattatcg cacgggtttt aacgattcat tactgggtat tcgttacagc ctgtcggatc
     1921 gtattcgtta ttactggccg catagccgga ttaaaaatag cgtcgaaacg atgatggtga
     1981 atctggaagg cgtggacatc ccactgggca tgattagtca gtatcttccg aaacaatttg
     2041 aacgcattca gtccggggaa ttatcagcaa taccgcatca gcttattatg gataaaattt
     2101 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
     2161 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
     2221 atgagatgct cgcgaaaggt gtggttcatg atacctggcc acaggcatta attaccagag
     2281 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata ccgcattgtg
     2341 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
     2401 ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt ggttattgcg ttgattgtgg
     2461 aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
     2521 atatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
     2581 atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta tttatgaaac
     2641 gcaaaattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
     2701 ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt cgggttaatg
     2761 aaatagaaac ctatatggat ggcgtgcatt tgatatgcac cactgccagg gtggatcgta
     2821 gttttggcaa tattccgtta gttcacggca tgccttttgt ttctggtgtc ggtatcgaag
     2881 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
     2941 gttatattct cgacctcggc cctacggtga tgctgccgat tgtcatcatt atgttttcta
     3001 aaatattagg catgaaggca ggcgattgct ttaaagcagg tctgcatatc gggattggct
     3061 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggcccg gcggcgaaag
     3121 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
     3181 cacccatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg attctggtta
     3241 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     3301 ggcatatgac cttcaccggc gcgttgctgc atctggcaac cggttcatgg atgataggga
     3361 tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     3421 atacccgaaa tttctttgag ctggaaggca ttgctattcc gcacggtacg tcggcgtata
     3481 tgggaccaat tgctgtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     3541 ttaaatttag tgccgacgat attcagcgca aatttggtcc gtttggcgag cctgtcaccg
     3601 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
     3661 tgcagctggc ggtaaaaacg gcggcggtta tgctgctgat gccacgggtg attaaaccca
     3721 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
     3781 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
     3841 tatcggcaag cctgattttt atcccgctca ccattttaat tgctgtttgt gttccgggta
     3901 atcaggtgct gccgtttggc gatcttgcaa ctatcggctt cttcgtggcg atggcggtcg
     3961 cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt atgagcatca
     4021 ccctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     4081 cgttaaaagc cggggggatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     4141 tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc ggcgcaattt
     4201 atctgaccgg tattttcatg acctggcgta gagcgcgtgg ttttattaaa caagagaaag
     4261 ccgttctcgc agaataattt ttacctgagg gggtggtaat cccccttaac aatcaggagt
     4321 ttttatgaaa tcagtggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     4381 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     4441 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
     4501 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac accccggcga
     4561 tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtc tgaaagggtt
     4621 ttattcccag tgcgcaaaat atgattttat tggctcacgg cgtgatggtg gatttgccga
     4681 atatattgtc gttaagcgaa acaatgtctt tgcactacct gcggatatgc ctattgagga
     4741 tggggctttt attgaaccga ttaccgttgg cctgcatgcg ttccatttag cgcaaggatg
     4801 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg caattcagtg
     4861 cgctgtcgcg ctgggagcaa agagtgtgac ggctatcgac attagctcag aaaaactggc
     4921 actggcaaaa tctttcggtg cgatgcaaac atttaacagc cttgaaatga gcgcgccgca
     4981 aatgcagggc gttttacgcg agctgcgctt taatcagctt atcctcgagg cggctggcgt
     5041 cccgcaaacc gtcgaactgg cggtagagat tgccgggcct catgcccagc tggcgctggt
     5101 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
     5161 agagctgacg gttatcggca gttggatgaa ctactccagc ccttggccgg ggcaggagtg
     5221 ggaaacggcg agtcggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     5281 ccgtggaagc tttgaaagct tcgcccaggc ggtgcgtgac atcgctcgta atgccatgcc
     5341 gggcaaagtg ttgctcattc cctgaaacct cgggccagtg agatgctggc ccggtattgt
     5401 gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     5461 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     5521 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     5581 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     5641 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
     5701 tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa tgatccacga
     5761 agggatgacc gttattctcg acagcggaag tacgacaatg cttatcgccg aaggattaat
     5821 gaccgccaaa aatatcacgg tgattaccaa cagtctcccg gcagcatttg ccctttccga
     5881 aaataaagac attactctgg tcgtctgcgg tggcacggtc cgccataaaa cgcgctcgat
     5941 gcatggctcc attgctgagc gttcattgca ggatattaat gccgatttaa tgtttgtcgg
     6001 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggct attcgattag
     6061 cggggcgatg gtcacagccg caaataaagt gattgccgtt ctcgattcat cgaaatttaa
     6121 tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     6181 tgctgtgtct gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     6241 gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt acggattctc
     6301 cctggttcaa cagagaatag tttgattaac gcaataatgg acaatctggt ggtaatcgac
     6361 aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac ggttcgccat
     6421 caagattaaa agtgatttcg tgtggggctt gtatatcgaa ccacgccgaa gcaccttcga
     6481 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
     6541 cgccggtgaa aatgcgcagt tgcagtaagc cgtcgttaat taatgcgttc gggcacaatt
     6601 gctgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     6661 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
     6721 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     6781 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
     6841 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     6901 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcagaattc
     6961 ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     7021 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacat
     7081 atcgtgcggc atcgcctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
     7141 gcaaccgcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     7201 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
     7261 tgtagagcag aaaagaaaag gtagagtcag aacaggatga aagtcggagg ataaaaagtc
     7321 agttcaagta tggaagattc agtggttacc accaggagct tgcaatgggg acagcgaata
     7381 ctaacggcag ccaccgataa atttcaaaaa agagcatata cctaatattc aactaa
//
LOCUS       DAZFRG010000222         7405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFRG010000222 DAZFRG010000000
VERSION     DAZFRG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.223"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(112..2685)
                     /gene="clpB"
                     /locus_tag="ACHKUC_003953"
     CDS             complement(112..2685)
                     /gene="clpB"
                     /locus_tag="ACHKUC_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO6620128.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLDVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2815..3546)
                     /gene="yfiH"
                     /locus_tag="ACHKUC_003954"
     CDS             complement(2815..3546)
                     /gene="yfiH"
                     /locus_tag="ACHKUC_003954"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO6620129.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMVVDAKSSTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3543..4523)
                     /gene="rluD"
                     /locus_tag="ACHKUC_003955"
     CDS             complement(3543..4523)
                     /gene="rluD"
                     /locus_tag="ACHKUC_003955"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO6620130.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4658..5395
                     /gene="bamD"
                     /locus_tag="ACHKUC_003956"
     CDS             4658..5395
                     /gene="bamD"
                     /locus_tag="ACHKUC_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO6620131.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5665..6006
                     /gene="raiA"
                     /locus_tag="ACHKUC_003957"
     CDS             5665..6006
                     /gene="raiA"
                     /locus_tag="ACHKUC_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO6620132.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6109..6234
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6110..6157
                     /gene="pheL"
                     /locus_tag="ACHKUC_003958"
     CDS             6110..6157
                     /gene="pheL"
                     /locus_tag="ACHKUC_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO6620133.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6256..>7405
                     /gene="pheA"
                     /locus_tag="ACHKUC_003959"
     CDS             6256..>7405
                     /gene="pheA"
                     /locus_tag="ACHKUC_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO6620134.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPV"
BASE COUNT         1899 a         1932 c         1743 g         1831 t
ORIGIN      
        1 aaaaaatata aaaactttat tgttcgctca tttttcaggc aaaacatctt taatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggc tcgttttatc attactggac
      121 ggcgacgatc cggtcttcat taacttccag gcgaataact ttacccggaa ccaattcacc
      181 agacagtatt tgctgtgcca gcgggttttc gatctgctgc tggatagcac gcttcagagg
      241 acgtgcacca tagaccggat cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga
      301 aatgtggatt tcataaccac gttcttccag acgtttgtac agacgtttca actgaatctg
      361 cgcaatcgac gcaatgtgtt gttcacccag cggatggaag acgacgactt catcgatacg
      421 gttaatgaat tccggacgga agttatggct taccacatcg agcaccagct ctttcatgtg
      481 cgcataatcc agttcaccaa aacgttcctg aatcagatcg gaaccgaggt tagaggtcat
      541 aatgacgacc gtattacgga agtcgaccgt tctcccctgc ccgtcagtca gacgcccatc
      601 atccagtacc tgcaacagaa tgttgaagac atccggatgc gctttttcca cttcatccag
      661 caggatgacg gaatacggac gacgacgcac cgcttcggtc agatagccac cttcttcata
      721 accaacatat cccggaggcg caccaaccag acgagacacc gagtgtttct ccataaactc
      781 ggacatatca atacggacca tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt
      841 acaaagctct gttttcccca cacccgttgg gccgaggaac aggaatgaac caatcgggcg
      901 atttggatcc gccagccccg cacggctacg acgaatagcg ttagataccg catcaaccgc
      961 ttcgttctga ccaataacgc gatggtgcag ttcttgctcc atacgcagca gtttttcgcg
     1021 ctcgctttcc atcatgcgag aaaccggaat tcccgtccaa cgcgccagca cttcagcaat
     1081 ttcggcatcg gtcactttat tacgcaacag acgcatagtt ttgccttcga gctgcgttgc
     1141 ggcttccagt tgcttttcca gttccgggat tttgccgtat tgcagttcag acatccgcgc
     1201 caggtcaccc acacggcgag cctgttcaat agcgattttc gcctgttcca gttccgcttt
     1261 aatggtctgc gtaccagaaa gcgaggcttt ctctgctttc cactcttctt ctaactcgga
     1321 gtactgacgt tctttgtcgc tcagttcttc gttgagcata tccaggcgtt ttttactggc
     1381 ttcatcagac tctttcatta acgcctgttg ttccagtttg agctggatga tacggcgatc
     1441 gagtcggtcg agttcttctg gttttgagtc aatctgcata cgaatgctgg atgctgcctc
     1501 atcgatcagg tcgatggctt tatctggcag ctgacggtca gcaatgtagc gatgagacaa
     1561 cgtcgccgct gcaacaattg ccgggtcagt aatttgcaca tggtggtgca gctcgtaacg
     1621 ttctttcagg ccacgcagaa tcgcaatggt atcttcaaca gaaggctcgg caacaaacac
     1681 tttctggaaa cgacgttcca gcgcagcatc tttttcgatg tactggcgat attcgtcaag
     1741 cgtcgtggca cctacgcagt gcaattcacc acgcgccagt gcaggtttca gcatgtttcc
     1801 ggcgtccatt gcgccatcgg ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa
     1861 caggatgacg ttgccttcct gtttagcaag atcgttaagt acgcctttta aacgttcttc
     1921 aaactcaccg cgatatttcg ccccagccac cagcgcgccc atatccagcg ccagtacgcg
     1981 gcggcctttc aacccttcgg gcacttcgcc gttgataata cgctgcgcca gaccttcaac
     2041 gatggcagtt ttaccgacgc cgggttcacc aatcaatacc gggttatttt tagtacgacg
     2101 ttgcagcacc tgaatggtac ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt
     2161 gccttgttcg gctcgttcgg taaggtcgat ggtatatttt ttcaaagcct gacgttggtc
     2221 ttcagcacct tgatcgttca cgctttcacc tccacgcatt tgttcaatcg cttgagtaat
     2281 gttggcggtg gtcgcccctg ctgctttcag gatgtcggcc agcgtgccgc gagactcaag
     2341 tgccgccaga acgaacagtt ctgacgagat aaagttatca ccacgttttt gcgccagctt
     2401 gtcgcaaaga ttaagaacgc gcaccagatc ctgtgatggc tggacatcac caccagtacc
     2461 ttcaacctgc ggtaaacgat ttaatgcctg attgatatct gtgcgcaact ggccagcatt
     2521 tatgccagcg gatgttaata aaggactaac cgaaccccct tcctgattca gcagggcgct
     2581 cattaaatga agtggttcga tgaattggtt gtcgtgcccg agtgcaagtg attgggcatc
     2641 ggcaagagca agctggaatt tattagtaag acgatccaga cgcataactc ctcccataac
     2701 ggatcaaagt tgctactgga gattaaatga ggtcatccct caattattca aggttattga
     2761 ccagattaat gtgaaaagaa aatcacgcgt accggatcgt cttgattctt taggttatat
     2821 cagccaaatg aaacttgcca tacgaccggt ggtcttgtcg cgacgataag agaagaaagt
     2881 ctcattttcc gtatatgtac aacggtcacc gccaaaaatt tgttcaacac caacgctcgc
     2941 cagacgctgc cgggcaagct gataaatatc cgccagatac ttatcaccat gctgaatgaa
     3001 agctgtactc gatttggcgt ctactaccat aaacgcctcg cgaacctccg cccccacttc
     3061 gaacgcacgt ggcccaattg ccggccctaa ccaggcgaga atattttccg gattatcagc
     3121 aaaacaggaa accgtctctt ccaacacgcc tgcgcacagt ccacgccagc cagcatgagc
     3181 ggcggcgacc tccgttcccg cacgattgca aaacagcaca gggaggcaat cggcagtcat
     3241 cactgcgcaa accgtgccgg gcgtattgct ataagaggca tctgctcgtt ttgaggcata
     3301 aggctcacca gtaagcttaa gcacatcttt gccgtgtacc tgctcaagcc agaccggttt
     3361 agaaggcaaa ttgcccgcag caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt
     3421 atcaccacaa tgagcgccaa ggttgagaga gtcgtaaggc ggcaagctca cgccgccgat
     3481 acgggtagag ctacaggccg caacaccttt tggcagcggc cactgcggga caatcagctt
     3541 actcataacc agtccacttc atccttatgc tcttcgaaat cggcgcgcat cacctcaatc
     3601 agctccacca tatcttgtgg aataggcgca tgccattcca tttcgatacc ggagatcggg
     3661 tgataaagac gcagcatggt tgcatgaagc gcctggcggt caaacttacg cagcgtggag
     3721 ataaacgctt ccgaagcacc ttttggcgga cgcggacggc caccataaac cggatcgccc
     3781 accagcggat gagtgatatg ggccatatgc acgcggatct ggtgcgtacg tccagtttcc
     3841 agacgcaacc gcagacgcgt gtgcacacgg aagtgttcca tgatgcgata gtgagtgacc
     3901 gccggtttac ccatcggatg caccgccatg tgagtacgtt tggtcgggtg gcggctgatt
     3961 ggttcatcta ctgtaccgcc agcggtcata tgaccaattg ccaccgcttc atactcacga
     4021 gtaatttcac gccgttgcaa agattcgact aaacgcgtct gagccggaac ggtttttgcc
     4081 acaaccatca ggccggtggt gtctttatcc agacgatgga cgatgcccgc acgcggtaca
     4141 tcggcaatgg gcggatagta atgaagcaac gcattcagta ccgtaccatc cgggttaccc
     4201 gcgccaggat gtaccaccag gtcgcgcggt ttattaatga cgatgatatc ttcatcttca
     4261 tagacgatat ccagcgggat atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg
     4321 ttgatggcaa cctgctcgcc acccaatact ttttcttttg gcttatcaca cactttgccg
     4381 ttaaccagca cacgctggtc gaggatccat tcttttatcc gcgaacgtga ataatccggg
     4441 aacatttcgg ccaaagcctg atctaagcgt tgaccgagtt ggttttcgga caccgttgca
     4501 gtgagctgta ctcgttgtgc catatatact gcttcttcgt ttatcgttgg ggttttacgg
     4561 ctttgccgtt taatatagtg tgctattgta gctggtctta accgggagca ggaacagaga
     4621 atctcccgta ttacattttg aggaaagtca aaacgtcatg acgcgcatga aatatctggt
     4681 ggcagccgcc acactaagcc tgtttttggc gggttgctcg gggtcaaagg aagaagtacc
     4741 tgataatccg ccaaatgaaa tttacgcgac tgcacaacaa aagctgcagg acggtaactg
     4801 gagacaggca ataacgcaac tggaagcgtt agataatcgc tatccgtttg gtccgtattc
     4861 gcagcaggtg cagctggatc tcatctacgc ctactataaa aacgccgatt tgccgttagc
     4921 acaggctgcc atcgatcgtt ttattcgcct taacccgacc catccgaata tcgattatgt
     4981 catgtacatg cgtggcctga ccaatatggc gctggatgac agtgcgctgc aagggttctt
     5041 tggcgttgac cgtagcgatc gcgatcctca acatgcacga gctgcgttta gtgacttttc
     5101 caaactggtg cgcggctatc cgaacagtca gtacaccacc gatgccacca aacgtctggt
     5161 attcctgaaa gatcgtctgg cgaaatatga atactccgtg gccgagtatt atacagaacg
     5221 tggtgcatgg gttgctgtcg ttaaccgcgt agaaggcatg ttgcgcgact atccggatac
     5281 ccaggctacg cgtgatgcgc tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa
     5341 tgcgcaagct gaaaaagtag cgaaaatcat cgccgcaaac agcagcaata cataacagaa
     5401 acctgaaaca caaaacggca gccttgagct gccgtttttt tattctgtta gttgtgaaac
     5461 tgaagcgatt tagtcactat cgatctcatc aaatatggct cgctttgaga tattcctcaa
     5521 gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca
     5581 aaattatgag atttccatca cacattttga catcaggaac gttatgctga attcaccaag
     5641 acgggaagac aagaggtaaa atttatgaca atgaacatta ccagcaaaca aatggaaatt
     5701 actccggcca tccgccaaca tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca
     5761 catctgatta atccacatat cattctgtcc aaagagccac aagggtttgt tgctgacgcc
     5821 acaatcaata cacctaacgg cgttctggtt gccagtggta aacatgaaga tatgtacacc
     5881 gcaattaacg aattgatcaa caagctggaa cggcagctca ataaactgca gcacaaaggc
     5941 gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa
     6001 gagtagtcct ttatattgag tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca
     6061 gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag gaaacaaaca tgaaacacac
     6121 accgtttttc ttcgcattct tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga
     6181 aacagaatgc gaagacgaac aataaggcct cccaaatcgg ggggcctttt ttattgataa
     6241 caaaaaggca acactatgac atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc
     6301 gcgctggatg aaaaattatt agcattactg gcagagcgac gcgaactggc cgtcgaggtg
     6361 ggaaaagcca aactgctctc gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg
     6421 ctggaaagat taattacgct cggtaaagcg caccatctgg acgcccatta cattactcgc
     6481 ctgttccagc tcatcattga agattccgta ttaactcagc aggctttgct ccagcaacat
     6541 ctcaataaaa ttaatccgca ctcagcacgc atcgcttttc tcggccccaa aggttcttat
     6601 tcccatcttg cggcgcgcca gtatgctgct cgtcactttg agcaattcat tgaaagtggc
     6661 tgcgccaaat ttgccgatat ttttaatcag gtggaaaccg gccaggccga ctatgccgtc
     6721 gtaccgattg aaaataccag ctccggtgcc ataaacgacg tttacgatct gctgcaacat
     6781 accagtttgt cgattgttgg cgagatgacg ttaactatcg atcattgttt gttggtctcc
     6841 ggcactactg atttatccgc catcaatacg gtctacagcc atccgcagcc attccagcaa
     6901 tgcagcaaat tccttaatcg ttatccgcac tggaagattg aatataccga aagtacgtct
     6961 gcggcaatgg aaaaggttgc acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa
     7021 gctggcggca ctttgtacgg tttgcaggta ctggagcgta ttgaagcgaa tcagcgacaa
     7081 aacttcaccc gatttgtggt gttggcacgt aaagccatta acgtttctga ccaggttccg
     7141 gcgaaaacga cgttgttaat ggcgaccgga caacaagctg gtgcactggt tgaagcgttg
     7201 ctggtactgc gcaatcacag tctgattatg acccgtctgg aatcacgtcc gattcacggt
     7261 aatccgtggg aagagatgtt ttatctggat attcaggcca atcttgaatc agcggaaatg
     7321 caaaaagcat tgaaagagtt aggggaaatc acccgttcga tgaaggtatt gggctgttac
     7381 ccaagtgaga acgtagtgcc tgttg
//
LOCUS       DAZFRG010000223         7392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFRG010000223 DAZFRG010000000
VERSION     DAZFRG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.224"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..294
                     /gene="chaB"
                     /locus_tag="ACHKUC_003960"
     CDS             64..294
                     /gene="chaB"
                     /locus_tag="ACHKUC_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO6620135.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            440..1135
                     /gene="chaC"
                     /locus_tag="ACHKUC_003961"
     CDS             440..1135
                     /gene="chaC"
                     /locus_tag="ACHKUC_003961"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO6620136.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1179..1532)
                     /gene="ychN"
                     /locus_tag="ACHKUC_003962"
     CDS             complement(1179..1532)
                     /gene="ychN"
                     /locus_tag="ACHKUC_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO6620137.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1717..3111
                     /gene="ychO"
                     /locus_tag="ACHKUC_003963"
                     /gene_synonym="iatA"
     CDS             1717..3111
                     /gene="ychO"
                     /locus_tag="ACHKUC_003963"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO6620138.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAASPFDNSNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQLLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3112..3762)
                     /gene="narL"
                     /locus_tag="ACHKUC_003964"
     CDS             complement(3112..3762)
                     /gene="narL"
                     /locus_tag="ACHKUC_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO6620139.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3755..5551)
                     /gene="narX"
                     /locus_tag="ACHKUC_003965"
     CDS             complement(3755..5551)
                     /gene="narX"
                     /locus_tag="ACHKUC_003965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO6620140.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5890..7281
                     /gene="narK"
                     /locus_tag="ACHKUC_003966"
     CDS             5890..7281
                     /gene="narK"
                     /locus_tag="ACHKUC_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO6620141.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1717 a         1815 c         1955 g         1905 t
ORIGIN      
        1 gttttggcaa gagtagatat tgctgaccac acttaatgtt caactttgta aaaggagtca
       61 acgatgccgt ataaaacgaa aagcgatctg ccggaaagcg taaagcacgt gcttccgtct
      121 catgcccagg atatctataa agaagcgttc aacagcgcat gggatcaata taaagataaa
      181 gaagatcggc gtgatgacgc cagtcgcgaa gaaacagcgc ataaagtggc ctgggctgct
      241 gtgaagcatg aatatgccaa aggggatgat gataaatggc ataaaaaatc gtaaaactaa
      301 agctattcgt gcggtgttgc cttgcaagtg gtccgtggat tgcatattgt cccgttagtg
      361 gtttcaaaat gagcagtaaa aatgtccgga agacaccaaa aagttgtcgc agggaagtat
      421 gcagtggcgg aggtgtaagg tgataacgcg tgatttcttg atgaatgccg attgtaaaac
      481 ggcatttggt gccattgaag aatcactctt atggtcagca gaacaacggg cggcttcgct
      541 ggcggcgacg ctggcttgtc gacctgatga gggaccggtg tggatcttcg gttatggatc
      601 gttgatgtgg aatccggcac tggagtttac cgagtcgtgc accggtacac tggttggatg
      661 gcatcgcgcg ttttgcctgc gcctgaccgc cgggcgcggg actgcgcacc agccgggacg
      721 aatgcttgca ctgaaagagg gcggacgcac cacaggcgtc gcctatcgac tgccagaaga
      781 gacgctggag caggaactaa ccctgctgtg gaagcgcgag atgattaccg gctgttatct
      841 gccaacctgg tgtcagcttg atcttgatga tggacgcaca gtaaacgcca ttgtgtttat
      901 tatggacccg cgacatccag aatatgaatc tgacactcgc gctcaggtca tagcgccgtt
      961 gattgcggcg gcgagcggtc cgctgggaac caatgcacaa tacctgtttt cactggaaca
     1021 ggagcttatc aaactgggaa tgcaggacga tgggctgaat gatttgctgg tatcggtaaa
     1081 aaaactgctg gcggagaatt atccggatgg tgtgttacgg ccgggattcg cctgagtaaa
     1141 cttccctcat agtggggcgt cagacgcccc ctcaaacatt aaaatgtgag cactttatcg
     1201 gctgacagcg tccattgcgc cagttcaaca agagtaccga tttccacccc atcaatcaga
     1261 ggaagtgtac taatcccgcg cccgtcggta caggttttgc acaatttcac cggtacattc
     1321 tgagcggtaa ggatctccag catttgctga atgttgtagc cttcccctgg tttttgcccg
     1381 cgcaacccgg ctgtgaccgc atcagacatc aggaacagac gcagatccag attgttctcc
     1441 tgctctcgta acgcaatggc cagccgcaag ctgttaaaca aggattcgct cccgtaaggt
     1501 gcgccattgg caacgatcac gattttttgc attatttact cctgtattca gggaattaga
     1561 cactcatctt ctatcttact gcttctgcag cgtctgacca atcggtcaca tttttaagga
     1621 ttttcctgaa agcgcgagaa aatacgacaa aaggtgccag taatcgttat tctttaaggc
     1681 tatggttttt catttttacc ggaagttacc gacgttttga gccgtttcgt tcctcgcatt
     1741 attccgtttt atttactctt gctggcggca ggcggtacag ctaacgcaca atctaccttc
     1801 gagcaaaaag cggcaagtcc ctttgataat agcaatgatg gtctgccgga tttaggcatg
     1861 gcacctgaaa atcatgatgg ggaaaaacac tttgctgaaa ttgtgaaaga tttcggcgaa
     1921 accagtatga atgataacgg gctggatact ggcgagcagg caaaagcttt cgcattggga
     1981 aaagtccgcg atgcgcttag tcaacaggtt aatcagcacg tagagtcctg gctatcaccg
     2041 tggggaaatg ccagtgttga cgtcaaagtg gataacgaag gacatttcac cggcagtcgt
     2101 ggaagctggt ttgtgccgtt acaagataat gatcgttatc tcacctggag ccagcttggt
     2161 cttactcagc aggatgatgg gctggtgagc aatgtgggcg ttgggcaacg ctgggcgcgc
     2221 ggcagctggc tggtgggtta taacactttt tatgacaact tgctggacga aaatcttcag
     2281 cgagcgggct ttggcgctga agcgtggggc gaatatttgc gactatcggc aaacttttat
     2341 cagccgtttg ctgcatggca tgaacagaca gccacgcagg aacaacggat ggcgcgcggg
     2401 tacgacctga cagcccggat gcgcatgccg ttctatcaac tcctcaatac cagtgtcagc
     2461 gtagaacagt attttggtga tcgtgtcgat ttgtttaact ctggtacggg ttatcacaat
     2521 cccgtcgcgt tgagtctggg attaaattac acccctgtgc ccttagtcac tgtgacggcc
     2581 cagcataaac agggtgaaag tggcgagaat caaaataacc tcgggctgaa tcttaactac
     2641 cgctttggtg taccgctcaa aaaacaactt tctgcgggcg aggttgccga aagtcagtcg
     2701 ttacgtggta gtcgctatga caatccgcag cgaaataatc tgccgactct tgagtaccga
     2761 cagcgaaaaa cgttaacggt gtttctggcg acacctccgt gggatctaaa acctggcgaa
     2821 acagtgccgc tgaaattaca aatccgcagt cgttacggta ttcggcaact gatttggcag
     2881 ggcgatacgc agatattaag tttgacgccg ggagcacaag ccaacagtga ggagggctgg
     2941 acgctgatca tgcctgactg gcaaaacggg gaaggcgcaa gcaatcactg gcgattgtca
     3001 gtggtggtgg aagataacca ggggcagcgt gtctcctcca atgagatcac gctaacgctt
     3061 gtcgaaccgt tcgacgcatt gtcaaacgac gaactgcgct gggaaccgta atcagaaaat
     3121 gcgctcctga tgcacccata ccgctgcttc cacgcgagac ttgagcttca ttttcttcag
     3181 catgtgcttg acgtgcactt ttactgtgct ttcggtgatg tccaggcggc gggcaatcat
     3241 cttgttcggc aaaccctggg caatcagctt gagaatatcg cgctcgcgtg gggttaactg
     3301 gttaacgtcg cgctcagtag tggcacggtt tgcgcgcaag ctggcggcca gaacaggcgt
     3361 taatgcttcg cttaatacca tttcgccagc agctgcctga tgtaacgctt tcagcagatc
     3421 ttccggttcc atatctttta acagatagcc atccgcgccg cgtttcagtg cggtgaccac
     3481 atcttcttca tggttagaga cgctgaatac cacaatgcga ccggagaggg acttttcgcg
     3541 cagtttatcc agcgtttcca gaccgttcat gccgggcata ttgagatcta acaggatcag
     3601 atcgggatca agagattccg ccagttcaat accctgttcg ccattactcg cttcgccaac
     3661 cacagtgata tctggtgcca tactgataag ctgttttacg ccagttcgca acatcgggtg
     3721 atcgtcgatc agcaggatag tagccggttc ctgattactc atgggtatct ccttggacgt
     3781 ctgtgaaagt tttttcggga ataaaggtga ccaccacttc ggtgccacct gattcacgac
     3841 ggcggacgcg gcaatcgcct cgtaaacttt gcgcacggtc gcgcattatt atcatgccgt
     3901 agtgattgct gcggatggca ttttcaggca cgccgcagcc gttatcctgg acggtcagtt
     3961 tgacctgatt atcgttttgc gccaccgtca ccacgacctc actcgcttgc gaatgtttga
     4021 gggcgttact taatgcctca cgggcaattt gcaacaagtg gattgcctga tgcgaaggca
     4081 ccagacgagg cggcaattga taatccagct tcaccgggaa gccaaatttg gcgctgtact
     4141 cttcgcaact cgcctccagc gccggacgta atccaggctc ggtgagctgc aaacggaatg
     4201 tggtgagcaa ttcacgcaac tgcacccagg atgcattcag ttcgttacgg atctgactta
     4261 acagttcgcg gctgctttct ggcagcgcat cgccctgcat ctgtaagcaa ctcacctgca
     4321 tcttcataca agagagagat tgggcaatag aatcatgcag ttcgcgcgca atggtggcac
     4381 gctcttccat cacgatcaac tgttgctgac gttcctgatg gcgatccagc gccagcgtgg
     4441 cggtgagttg ttcaaccagg gtatccacca gttgttgttg atcatggcta agatgacgcc
     4501 cctgcggcag ggtcgccagc aaaataccgt actgcgtatg agtgtcagcc agccgccact
     4561 tcagggttgt gccgcgatcg ccaacgggta atacgccgcg cgggcagagc tggcagcctt
     4621 tatcatcaca agtcatatct gactggcagg taaactcctg atgattctct tcatcatcag
     4681 tgtcatacac ccgcagttcg atatcacgta gcagggttaa attctgtaag tcgttgagta
     4741 caggtgacag gcgttcacac agcggggcgc gggaatgcaa acggcggtta gcctgccata
     4801 aaaaagagag gatctgattt ttatgctcca gcccggcggt tttctcctga acccgctgct
     4861 caagtacggc ataactttcc gccagttctg cagacatatt gttcaacgca gttccaagca
     4921 tcgccatttc gttgcgcccg ctgatgtttg cgcgttgggt aaaatcgcga tggctgacgg
     4981 cactcgccat tgccagcagt tgccgccacg gttgtagcaa tctcgcccgc aaccagataa
     5041 tagtgaatac cagtaaaagt gccataaata ccgccattac ccgatggacc agcaccactg
     5101 tctcaatgcg catttccgtg gtgcgatcaa aaccagatac cagctgatca agcccggcaa
     5161 caaactggct gacatccgct gacaccgttt cgcggttttg tgcacgtatc agtgcaggga
     5221 tcagttcatt acgccagtaa tcttgtaaac cctgtaattg cgccagttgc ccgtctcgtt
     5281 ctgctgctcg agtcaactcg gcgctaaatg ctgtttgttc catctcttta attaagggct
     5341 tgtctttctc gcttaatggc actgctgcca acagacggta actttgcatg cgcagcgatc
     5401 ccgctttgtt gatcgcatgg gcgctgccct gaacgccttg caccagccag ccagaaaccg
     5461 ccatccctgc cagtccaata gcagtagaaa gcaacacaat aagcgcaacc tgattaacca
     5521 gggtgagcgg agagagacaa cgtttaagca tgtaaacctc ttccttcagg ctttaaatga
     5581 gcaataacct taatgaatgt gatgatacat tctggaatgg cagtattctc ggctattggc
     5641 tgaagtatac ccatacccgg aaagagttac tccttatttg ccgtgtggtt agtcgcttta
     5701 catcggtaag ggtagggatt ttacagcacc gtgaaaaatc tcataatttt tatgaaatca
     5761 ctgtactcac tatggataat gataaatatc aatgatagat aaagttatct tatcgtttga
     5821 tttacatcaa attgccttta gctacagaca ctaaggtggc agacatcgaa acgagtatca
     5881 gaggtgtcta tgagtcactc atccgccccc gaaagggcta ctggagctgt cattacagat
     5941 tggcgaccgg aagatcctgc gttctggcaa caacgcggtc aacgtattgc cagccgcaac
     6001 ctgtggattt ccgttccctg tctgctgctg gcgttttgcg tatggatgtt gttcagcgct
     6061 gttgcggtga acttaccgaa agtcggtttt aattttacga ccgatcagct atttatgttg
     6121 actgcgctgc cttcggtttc tggcgcgtta ttacgtgttc catactcctt tatggttcct
     6181 atctttggtg gtcgtcgctg gacggcgttc agcaccggta ttctgattat tccttgcgtc
     6241 tggctgggtt ttgccgtgca ggatacctcc acgccttata gcgtcttcat catcatttct
     6301 ctgctgtgcg gctttgctgg cgcgaacttc gcatccagta tggcaaacat cagcttcttc
     6361 tttccgaaac agaagcaggg tggcgcgctg ggtctgaatg gtggtctggg caacatgggc
     6421 gtcagcgtca tgcagttggt tgctccgctg gtggtatcac tgtcgatttt cgcagtattt
     6481 ggtagccagg gcgtcaaaca gccggatggg actgagctgt atctggcgaa tgcgtcctgg
     6541 atatgggtgc cgttccttgc catcttcacc attgcggcgt ggtttggcat gaacgatctt
     6601 gctacctcga aagcctccat caaggagcag ttgccggtac tcaaacgggg tcatctgtgg
     6661 attatgagcc tgctgtatct ggcaaccttc ggctccttca tcggcttctc cgcgggcttt
     6721 gcgatgctgt caaaaacgca gttcccggat gttcagattc tgcaatacgc tttcttcggg
     6781 ccgtttattg gtgcgctggc gcgttctgca ggtggtgcat tatctgaccg tctgggcgga
     6841 actcgtgtca cgctggtgaa ctttatcctg atggcgattt tcagcggcct gctgttcctg
     6901 accttaccga ctgacggaca gggcggaagc ttcatggcgt tcttcgcagt cttcctggcg
     6961 ctgttcctga cagctgggct gggtagtggt tccactttcc agatgatttc cgtgatcttc
     7021 cgtaaactga caatggatcg cgtgaaagca gaagggggtt ctgacgaacg tgcgatgcgt
     7081 gaagcggcaa ccgacacggc ggcggcgctg ggtttcatct ctgcgattgg cgcgattggt
     7141 ggcttcttta tcccgaaagc gtttggtagc tcgctggcat taacgggttc gccagtcggc
     7201 gcaatgaaag tatttttgat tttctatatc gcctgcgtgg tgattacctg ggcggtatat
     7261 ggtcggcatt ctaaaaaata aatcgtaata tttgattatc ctttgcgcgg catgatgtcg
     7321 cgcttttttt atgcgtcatt tagttacaac atactaatgt tgtatggctt atttcgctgg
     7381 atttcattaa ga
//
LOCUS       DAZFRG010000224         7362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFRG010000224 DAZFRG010000000
VERSION     DAZFRG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.225"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..1188
                     /gene="anmK"
                     /locus_tag="ACHKUC_003967"
     CDS             79..1188
                     /gene="anmK"
                     /locus_tag="ACHKUC_003967"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO6620142.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLSGNLPSVTGASQETVLGAIFPANP"
     gene            1292..1615
                     /gene="mliC"
                     /locus_tag="ACHKUC_003968"
     CDS             1292..1615
                     /gene="mliC"
                     /locus_tag="ACHKUC_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO6620143.1"
                     /translation="MKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTVKL
                     NNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNCQL
                     QNPQR"
     gene            1674..2330
                     /gene="pdxH"
                     /locus_tag="ACHKUC_003969"
     CDS             1674..2330
                     /gene="pdxH"
                     /locus_tag="ACHKUC_003969"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO6620144.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2459..3733
                     /gene="tyrS"
                     /locus_tag="ACHKUC_003970"
     CDS             2459..3733
                     /gene="tyrS"
                     /locus_tag="ACHKUC_003970"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO6620145.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3795..4655
                     /gene="pdxY"
                     /locus_tag="ACHKUC_003971"
     CDS             3795..4655
                     /gene="pdxY"
                     /locus_tag="ACHKUC_003971"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO6620146.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(4699..5304)
                     /gene="gstA"
                     /locus_tag="ACHKUC_003972"
     CDS             complement(4699..5304)
                     /gene="gstA"
                     /locus_tag="ACHKUC_003972"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO6620147.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5410..6912)
                     /gene="dtpA"
                     /locus_tag="ACHKUC_003973"
     CDS             complement(5410..6912)
                     /gene="dtpA"
                     /locus_tag="ACHKUC_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO6620148.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         1862 a         1804 c         1913 g         1783 t
ORIGIN      
        1 attggtcatt ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg caaacgtcag
       61 attgaaaaag gatgaactat gaaatcgggc cgctttattg gcgttatgtc aggcaccagc
      121 cttgatggtg ttgatgttgt gttggcgaca attgatgaac accgggtcgc acagctggca
      181 agtttgagct ggccgatccc ggtgtctctg aaacaggctg tactggatat ttgccagggc
      241 cagcagctta cactttcgca gtttggacag cttgatactc aactcgggcg actttttgct
      301 gatgcggtca atgccttgct taaggaacaa aacctgcagg cgagagatat cgttgcgatc
      361 ggctgtcacg gtcaaaccgt ctggcacgaa ccgacgggcg tggcaccaca cactttacag
      421 attggcgata acaatcaaat tgtggcgcgc accggaatta cggttgtcgg tgattttcgc
      481 cgtcgcgata ttgccttggg aggacaaggt gcaccgctgg tacctgcgtt ccatcatgcg
      541 ctgctggctc acccaaccga gcgacgaatg gtgctcaata ttggcggcat cgccaatctg
      601 tcactgctca ttcctggcca gccggttggg ggctacgata ccggtcctgg taacatgctg
      661 atggatgcct ggatctggcg tcaggccggt aaaccttacg ataaagatgc cgagtgggca
      721 cgggcgggta aagttattct cccactgctg caaaatatgc tcagcgaccc atatttctcg
      781 caacccgcac cgaaaagcac cggacgcgaa tactttaact acggctggct ggagcgccat
      841 ttgcgccatt ttccgggggt tgatccccgt gatgtgcagg ccacagtggc agaactcacc
      901 gccgtgacca tttctgaaca agttttgttg agcggtggct gcgaacgatt gatggtatgt
      961 ggtggaggta gtcgtaatcc gctactcatg gcgcgtctgg cggcattact gccaggcaca
     1021 gaagtcacca ccaccgatgc cgttggcatt agtggcgatg acatggaagc attggcgttc
     1081 gcctggcttg cctggcgaac gctggcggga ttatcaggaa atctgccttc cgtcactggc
     1141 gcaagccagg agacggtact gggggctatt ttccccgcta acccgtgata tcagagttaa
     1201 ctgaattttt cttaccgcgt ctgccgttac acttggaacg ttagggaggg cgtattgccc
     1261 tccagaccag gaaagtcttc gggatatttc tatgaaaaaa ctgttactga tctgtttgcc
     1321 ggtgcttctc accggctgta gcgcgttcaa tcaactggtt gagcgcatgc aaaccgatac
     1381 gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc cgcgccagga
     1441 agtcagtttt gtttacgata atcaactact gcatctcaaa cagggcattt cagcgtctgg
     1501 tgcacgttac accgacggaa tctatgtttt ctggtcgaaa ggcgaggaag cgactgtcta
     1561 taaacgcgac cgcatcgtct tgaataactg tcagttacaa aatccgcagc gttgagattt
     1621 ttccaggggc ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa accatgtctg
     1681 ataacgacga attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa ggcgggttac
     1741 gccgccgcga tctacccgcc gatccattaa ccctttttga acgttggctc tctcaggctt
     1801 gtgaagccaa actggctgac cctaccgcga tggtggtcgc taccgtggat gaacatggtc
     1861 agccttatca gcgcatcgtt ttactcaaac attacgacga aaaaggcatg gtgttttaca
     1921 ccaacctcgg cagccgtaaa gcacatcaaa tcgaaaataa tccgcgcgtt agcctgctgt
     1981 tcccgtggca tacccttgaa cgccaggtga tggtgatcgg taaagcggaa cgactttcga
     2041 ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga tagccagatt ggtgcatggg
     2101 tttcgaagca gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa tttctggagc
     2161 tgaagcagaa gtttcaacag ggcgaagtgc cattgcccag tttttggggc ggttttcgcg
     2221 tcagccttga acagattgag ttctggcagg gcggtgagca tcgcctgcat gaccgctttt
     2281 tgtaccagcg tgaaaatgat gcgtggaaga ttgatcgtct tgcaccctga aaagatgcaa
     2341 aaatcttgct ttaatcgctg gtactcctga ttctggcact ttattctatg tctctttcgc
     2401 atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg agattttgat
     2461 ggcaagcagt aacttgatta aacaattgca agagcggggg ctggtagccc aggtgacgga
     2521 cgaggaagcg ttagcagagc gactggcgca aggcccgatc gcgctctatt gcggcttcga
     2581 tcctaccgct gacagcttgc atttggggca tcttgttcca ttgttatgcc tgaaacgctt
     2641 ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc tgattggcga
     2701 cccgagcttc aaagctgccg agcgtaagct gaacactgaa gaaactgttc aggagtgggt
     2761 ggacaaaatc cgcaagcagg ttgccccgtt cctcgatttc gactgtggag aaaactctgc
     2821 tatcgcggcg aacaactatg actggttcgg caatatgaat gtgctgacct tcctgcgcga
     2881 tattggcaaa cacttctccg ttaaccagat gatcaacaaa gaagcggtta agcagcgtct
     2941 caaccgtgaa gatcagggga tttcgttcac tgagttttcc tacaacctgt tgcagggtta
     3001 tgacttcgcc tgtctgaaca aacagtacgg tgtggtgctg caaatcggtg gttctgacca
     3061 gtggggtaac atcacttctg gtatcgacct gacccgtcgt ctgcatcaga atcaggtgtt
     3121 tggcttgacc gttccgctga tcaccaaagc agacggcacc aaatttggta aaactgaagg
     3181 cggcgcagtc tggttggatc cgaagaaaac cagcccgtac aaattctacc agttctggat
     3241 caacactgcg gatgccgacg tttaccgctt cctgaaattc ttcaccttca tgagcattga
     3301 agagatcaac gccctggaag aagaagataa aaacagcggt aaagcaccgc gcgcccagta
     3361 tgtgcttgca gaacaggtga cacgtctggt gcatggtgaa gaaggtttgc aggcggcaaa
     3421 acgtattacc gaatgcctgt tcagcggttc cttgagtgcg ctgagtgaag cggacttcga
     3481 acagctggcg caggacggcg taccgatggt tgagatggaa aagggcgcag acctgatgca
     3541 ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa ctatcgcctc
     3601 caatgccatc accattaacg gtgaaaaaca gtccgatcct gaatacttct ttaaagaaga
     3661 agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt actgtctgat
     3721 ttgctggaaa taatgcatta agtggaaagg gggagtgaga aatcactccc cctggttttt
     3781 atacagggaa catgatgaaa aatattctcg ctatccagtc tcacgttgtt tatggtcatg
     3841 cgggtaacag tgcggcagag tttccgatgc gccgcttggg cgcgaacgtc tggccgctga
     3901 acaccgttca attttctaat cacacccaat acggcaaatg gactggctgc gtgatgccgc
     3961 ccagccattt aaccgaaatt gtgcaaggca ttgccgccat tgataaatta cacacctgtg
     4021 atgccgtatt aagtggctat ttgggatcgg cggagcaggg tgaacatatc ctcggtatcg
     4081 ttcgtcaggt gaaagcagcg aatccacagg cgaaatattt ttgcgatccg gtaatgggtc
     4141 atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg cggcacggtt
     4201 tgcctgccag cgatatcatt gcgccgaatc tggttgagct ggaaatactc tgtgagcatc
     4261 cggtaaataa cgtcgaagaa gcggttctgg cagcgcgcga actcattgcg caagggccac
     4321 aaattgtgtt ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt tttgaaatgc
     4381 tgctagtcac cgccgatgaa gcctggcata tcagccgtcc gctggtggat tttggtatgc
     4441 gccagccggt aggtgttggt gatgtgacga gcggcttact gctggtgaaa ctgcttcagg
     4501 gggcaacgct gcaggaggcc ctggaacatg tgaccgctgc agtatacgaa atcatggtga
     4561 ccacaaaagc aatgcaggaa tatgagctgc aagtggttgc tgctcaggat cgtattgcca
     4621 atccagaaca ttatttcagc gcaacaaagc tctgaaatat ctcatttcgg cccacaacga
     4681 cctgtgggcc gatggctatt actttaagcc ttccgctgac agcgcgtctt gtacttccgg
     4741 acgttcagcc atacgttgca taaatgccgc aatgtgctct aacccttcca gattcagttt
     4801 caccgcgtat gcccagcgca gaaccgtaaa cagataggca tcagcaattg taaatcgttg
     4861 cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca tattgcagct tcttctccag
     4921 ctgcgcgcga actgtcgatt tgtactcttc cggtgtatca gggcgaaaca gaggtgtgaa
     4981 acctttatgc agctcggtgg cgatgtaatt cagccattcg atggttttat agcgggaaat
     5041 actgtttacc ggtgccagca actggcggtc ggggacgctg tcggcaagat actgcataat
     5101 cgctacgcct tccgtcagca aagtaccatc gtccagcagc aatgcaggta cctgtccctt
     5161 agggttaacg gcaaagtaat cgtcaccgtt ttcgagacgt tttttcatta aatccacact
     5221 gacgagcgta aaatcctttc cgctctcacg cagggtgata tgggaagcga gagagcaggc
     5281 gcccggtttg tagaacaatt tcatcggtaa ctccttttgt ctgtggttta aagtatggta
     5341 gtgcgttgct gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt aaaagagttt
     5401 ccctgaattt tacgctacgg ctgctttcgc cgctttgtct gcagcgtcat cctgcgtcat
     5461 gcggtgcagt ttcggcgcgg taaacagcat cagtactgca ataacggcag tagcgacacc
     5521 aatctgcaag aatacgcgac catagacttc cagtgacatc agcggatcgg taacgttatc
     5581 cggcacagcc atcatacctg caacataacc accaatcagg tttgcaccgg cagtggtcag
     5641 gaaccagcta cccataatga agcccatcag acgctgcgga acgagttgtg caaccattgc
     5701 cagacccagg ccagagatca tcagttcccc gatactctgc aggccatagc tcgcgaccag
     5761 ccagcttaca gacacgatac cagcgtcaga cgcgaatttc gcacccagcg gcagaatcag
     5821 gaacgcacca gaacacatca ccatgccgat agcaaacttg gttggcattg gcagggtatc
     5881 gcccatcttg ttatagatag cggccagaat cgggctacca atgatgatcc agaacgggtt
     5941 cagtgcctga tactgctcag gttctacggc caggcccaga atggtgtgtt caacgttacg
     6001 aatcgcaaag aagttcagtg acgttggcat ctggctataa agcacgaaga agataatggc
     6061 ttcgagcatc aggatgaagg caacgatcat tttacgacgc gcagcacctt tcatcgcgaa
     6121 ggcttcttta ccgaagataa ccacgatacc gaaggcaaca acgcccagcg ccatacgcgc
     6181 aacttcctga ttgtgcagca gccaggtggc gatagcgatc agtgccacaa caccaataat
     6241 ggtcagcagc aggttacggt agttgatagg ctcgaagtct ggttttgaac cgtactgttt
     6301 aacccaacgt tggcagaagg cgaagttaac gatagtgatc agcaggccta caacgctcaa
     6361 cgcaaacgca acactccagc cgtatttcgc ggccagccac ggcgtagcaa tcatagagaa
     6421 gaaagagccg atgttgacgg acatgtagta catggtgaat gcaccgtcca gacgcgggtc
     6481 gtttttctca tagcatgtag aaagcagaga agacgggtta gctttaaaca ggccgttacc
     6541 gaccgcaata gccgccatac ccatataaac gataccggcg tcgtgaccag accaggcaac
     6601 cagagcataa ccaatcgcca gcacaatagc gccgagcata attacgcgtt tagtacccag
     6661 taccttgtca cctaaccagc cgccgatagc gaccagacca taaaccaggg cactaaagga
     6721 agagaaaagg gtgattgaat ccgcttcaga catacccagt tgtttaacca ggtaaacagc
     6781 cataattcct tgtaggccgt aataaccaaa acgttcccat aactcaatcg agaagatgag
     6841 atagaacgcc ttcggttgtt taaaagcgtt caaactgacg ctttcagttg gtttttggtt
     6901 tgcagtggac acttttacct ctttttttac atcccatatt aacgggggtg ctttctcgcg
     6961 ttatgtctaa aaaacatacg cgcaattgtt atagtgggag gggaaacggc aggtaatgtt
     7021 cactatcttg cactttcaga caagtccttt gtaataatct gttacatata actgaggcgg
     7081 ggaattcaac cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc
     7141 agattttttc actaccctga gagggtgatg atggcttaat ggcgatatgt tctactattt
     7201 ggcgtttttc ctagtgagat agtccagttt ctgaaaaata gccagtgtaa tgttttgtag
     7261 gtcaatattt gttgcgtgtg taaatttaaa tgattttaac ctgattcttg caatgttttt
     7321 acattgccac agcatcgtga tcttgatcac gaatgaatag aa
//
LOCUS       DAZFRG010000225         7353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFRG010000225 DAZFRG010000000
VERSION     DAZFRG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.226"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..73)
                     /locus_tag="ACHKUC_003974"
     tRNA            complement(1..73)
                     /locus_tag="ACHKUC_003974"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Arg,seq:acg)
     gene            complement(138..214)
                     /locus_tag="ACHKUC_003975"
     tRNA            complement(138..214)
                     /locus_tag="ACHKUC_003975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Arg,seq:acg)
     gene            complement(218..310)
                     /locus_tag="ACHKUC_003976"
     tRNA            complement(218..310)
                     /locus_tag="ACHKUC_003976"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(274..276),aa:Ser,seq:gct)
     gene            complement(626..811)
                     /gene="csrA"
                     /locus_tag="ACHKUC_003977"
     CDS             complement(626..811)
                     /gene="csrA"
                     /locus_tag="ACHKUC_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO6620149.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1046..3676)
                     /gene="alaS"
                     /locus_tag="ACHKUC_003978"
     CDS             complement(1046..3676)
                     /gene="alaS"
                     /locus_tag="ACHKUC_003978"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO6620150.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3805..4305)
                     /gene="recX"
                     /locus_tag="ACHKUC_003979"
     CDS             complement(3805..4305)
                     /gene="recX"
                     /locus_tag="ACHKUC_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO6620151.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4374..5435)
                     /gene="recA"
                     /locus_tag="ACHKUC_003980"
     CDS             complement(4374..5435)
                     /gene="recA"
                     /locus_tag="ACHKUC_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO6620152.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5515..6012)
                     /gene="pncC"
                     /locus_tag="ACHKUC_003981"
     CDS             complement(5515..6012)
                     /gene="pncC"
                     /locus_tag="ACHKUC_003981"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO6620153.1"
                     /translation="MTDSELMQLSEQVGQALKVRGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6157..7242)
                     /gene="mltB"
                     /locus_tag="ACHKUC_003982"
     CDS             complement(6157..7242)
                     /gene="mltB"
                     /locus_tag="ACHKUC_003982"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO6620154.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1707 a         2074 c         1786 g         1786 t
ORIGIN      
        1 gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct
       61 gagctacgga tgcatcggga aacttacttt actgcagatt ttttgatacc gccactaaag
      121 tcgtatcaag taagagatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
      181 agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa
      241 ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc agcctctcgg
      301 ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc
      361 tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc ccacactttt
      421 atcgtttgca cacttcatgc tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca
      481 acagaaaaac ggaggtctga cagatagcag taatgcaaaa aaatggagac ttaagttgaa
      541 tgaacgggag taaagcgaaa agacaatgga gtgaaagaga aattttgagg gtgcgtctca
      601 ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt tcagcctgga
      661 tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt acgccaatac
      721 gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca atcatgaggg
      781 tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat taaaagagtc
      841 gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag caatgacaaa
      901 cacattacat ctaagcagtc atggcattac attctgttaa acctaagttt agccgatata
      961 cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag
     1021 tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag cctttcacac
     1081 tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac
     1141 gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc cctgctttca
     1201 cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct tcggctaccg
     1261 ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc atggtacgca
     1321 acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca ttaacatcaa
     1381 ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta agctgttgta
     1441 actctttttc cagctgacgc gtacgttcca gtactgaacg cactttatca gccagattat
     1501 tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg
     1561 tggcgatagc gccttctccg gtcaccgctt cgatacgacg aacgcctgca gcagtacccg
     1621 attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga gtaccgccac
     1681 acagctcggt ggagaaatcg cccatgctca gcacgcgtac gcgctcatca tacttctcgc
     1741 caaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg ttggtttcga
     1801 tcggcaagtt acggcgaatc tgtgcgttca ccaggtcttc gaccgcacga atctcttctg
     1861 gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac tttgtcgtta accagtgaac
     1921 ctttctgcga tacatgagta cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg
     1981 cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt
     2041 cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc tggccgtatt
     2101 tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct ttatcaccaa
     2161 cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca gcttcctggc
     2221 ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact ttgccgttca
     2281 gttccagatg gtcatagcct ttaaattcag atgcactgtc aacacggatc attgcgttgt
     2341 aatcggcacc aaagccgctg gcttcgcgcg cacgacgacg ctgctcttcc attgctgctt
     2401 caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa
     2461 ccgggaagcc gtaggtgtcg tacaggcgga aagcagtttc accatccagc gtatcaccag
     2521 aaagttttgc cagctcttca tccagcagcg ccagaccgcg ctccagagta cgggcaaact
     2581 gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac
     2641 ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg
     2701 cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac
     2761 ggttttcgtt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt
     2821 cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact ttcgctaccg
     2881 cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg
     2941 cagcaatacg ctccagaccc ataccggtat ctacagacgg tttcggcagc ggttccatcg
     3001 tgccatcggc ctggcggttg aactgcatga agacgatgtt ccagatctca atgtagcggt
     3061 cgccgtcttc ttccggactt cccggagggc cgccccaaat gtggtcgccg tgatcgtaga
     3121 agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag ttgtcagatg
     3181 cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg atccctactt
     3241 ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc cacagacgct
     3301 ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat
     3361 cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag gtatggtgac
     3421 gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt
     3481 gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca tccttgaact
     3541 ggttcatccc ggcgttggta aacaacagag ttgggtcgtt atggggtacc agggagctgc
     3601 tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga cggatctcag
     3661 cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgct accagccgtt
     3721 gcgtgtcgtt aaccgcccgg ctggtaactg aaaagtggga ataagataag ttttcttgac
     3781 tgggaagtaa aatcccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc
     3841 ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac
     3901 agttggcaaa ggttcgccat attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc
     3961 gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag
     4021 ttcctgacga atacgcgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc
     4081 aacaaatcgg ctgtcatcaa gatagccatg ttcatggcac caggcaataa cgcgctcgta
     4141 atcttctgcc gtggcatcaa tctcttccgg gccatttttg cccataatcg gtgccgcgag
     4201 tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc
     4261 caacaggcga gcatatgccg ggcgacggga tgttgattct gtcatggcat atccttacaa
     4321 cttaaaaaag caaaagggcc gcagatgcga cccttgtgta tcaaacaaga cgattaaaaa
     4381 tcttcgttag tttctgctac gccttcgctg tcatctacag agaaatccgg cgttgagttc
     4441 gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttctgggtta
     4501 tctttcagcc aggcagtcgc attcgcttta ccctgaccga tcttctcacc tttgtagctg
     4561 taccacgcgc ctgctttctc gataagcttc tctttcacgc ccaggtcaac cagctcgccg
     4621 tagaagttga taccttcgcc gtagaggatc tggaattcag cctgtttaaa cggcgcagcg
     4681 attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc
     4741 accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca
     4801 ccggtagtgg tttccgggtt accaaacatc acaccaattt tcatacggat ctggttgatg
     4861 aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc
     4921 atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc
     4981 ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgtgccagg
     5041 gcgtcacaga tttccagtgc ctgctcgccg gtatccggct gggagcacag caggttgtcg
     5101 atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac
     5161 gcacaggttt taccttcgcg ctgcgctgcg gcgatcacct gcaacgtcag cgtggtttta
     5221 ccggaagatt ctggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca
     5281 agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct
     5341 tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc
     5401 gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat
     5461 accggatagt caatatgttc tgttgaagca attatactgt atgctcatac agtatcaagt
     5521 gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct gacgacgcac
     5581 cgcatcacgg tcaccgctga agcattcccg ccgggtaatg ccttcaccac gggcagtggc
     5641 aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg gcccggcgat
     5701 accactaata gacacggcgt aatcagcacg agccgctttc agtgcgccta tcgccatttc
     5761 caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc gcacgccgat
     5821 catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc aggcggagct
     5881 accggcaata tcggtaatca ctttcgctac ccaaccgccg gtacaagact cagcagttgt
     5941 tacggttgcg ccacggactt tcagcgcctg cccaacctgt tcgcttaact gcatcagttc
     6001 actgtcagtc atcacaacct ctgtcagtca taaatttatg ccggacaaga tagcactttc
     6061 gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa aagctgatta
     6121 gccagaggga agctcacgcc cccctctggc aaatagttac tgcacgcgcg ccagcgccac
     6181 ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgtggttat aacgggtaat
     6241 ggtgtagaag ttcggcagac cgtaccagta ctggtaaccg gtaccgacat ccagacgcag
     6301 caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg ccgcggcaag
     6361 ctgcgaaatg ctgtacctgg ttttgaagcc atttggcaag cctggcgcct gaccgtttgc
     6421 cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaataat tcgccacgct
     6481 accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga aatctaccgc
     6541 atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg caaaggaacc
     6601 tttcagattg agcggatcgt cctgctcgtc gcgcgccatc agcaggaagg tttccagctc
     6661 gccagaaaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag
     6721 gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa tcccgacgat
     6781 aatttccggc ggtacaccat acacctgcca cgcgcgattc aacgcatctt catactgatt
     6841 ccagaaaacc acaccgttct gcacgttgtc cggcgtaata aattttttgc gataacgcag
     6901 ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct ggttatccat
     6961 cagccgcagt accgaatcca gacgtttcgc ctgggagaga atttcctgca actgctgacg
     7021 atcgaaaccg tgtttgttca ccattttgtc gatgaactgc tgggcattcg ggttattggc
     7081 gaagtcaccg cccatctgca tcacattgtg ctgcggctca agcaggaagc cgccagacgg
     7141 cgttccggtg gtcgtttcag tctcagtagg ttttggcttg ctgctacagg cggcaagcaa
     7201 cacaaaaagg ggaagcaatg ttacataacg acgcttgaac atgaggggtc catttaacag
     7261 attcaaccaa gggcaagtat ggtaaagcat cccgccccgc acaaggaagc ggtagtcact
     7321 gcccgatacg gactttacat aatcaactca ttc
//
LOCUS       DAZFRG010000226         7317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFRG010000226 DAZFRG010000000
VERSION     DAZFRG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.227"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..880)
                     /locus_tag="ACHKUC_003983"
     CDS             complement(47..880)
                     /locus_tag="ACHKUC_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620155.1"
                     /translation="MSDGWLDSTMQAINDRIKSPLWGYIILAWVWFNWPNLAMLFMSD
                     APVKFRIDYILSQEYFYAHYLLAPVFFGGVLAVITPYAQWLLSLAQKWATDKHNENVY
                     LTKEKEYLDSIRLTGLKVRAAREEEKENAKIDADIKAEVERGKREELVTEELQTEKKL
                     IQKEINNLKQLVSKEKQTIENIEIEKERLQDLIVASLEVMDDFFKVDNSHSLQQLKSR
                     VEELLTVSDIEASTIRNALRQKKELTSSQTMKMFDMVEAKIKKEKEKGNNIESNELIN
                     Q"
     gene            974..1321
                     /locus_tag="ACHKUC_003984"
                     /pseudo
     CDS             974..1321
                     /locus_tag="ACHKUC_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1374..1769
                     /gene="yecN"
                     /locus_tag="ACHKUC_003985"
     CDS             1374..1769
                     /gene="yecN"
                     /locus_tag="ACHKUC_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO6620156.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1810..2553
                     /gene="cmoA"
                     /locus_tag="ACHKUC_003986"
     CDS             1810..2553
                     /gene="cmoA"
                     /locus_tag="ACHKUC_003986"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO6620157.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2550..3521
                     /gene="cmoB"
                     /locus_tag="ACHKUC_003987"
     CDS             2550..3521
                     /gene="cmoB"
                     /locus_tag="ACHKUC_003987"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO6620158.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(3686..6114)
                     /gene="torZ"
                     /locus_tag="ACHKUC_003988"
                     /pseudo
     CDS             complement(3686..6114)
                     /gene="torZ"
                     /locus_tag="ACHKUC_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(6139..7239)
                     /gene="torY"
                     /locus_tag="ACHKUC_003989"
     CDS             complement(6139..7239)
                     /gene="torY"
                     /locus_tag="ACHKUC_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO6620159.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTD
                     LTVVDASGKQPIVRLQGYQMQGSENTLYLAVGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDGTYCAGCHAPIAADHYTVNTWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1703 a         1866 c         1677 g         2071 t
ORIGIN      
        1 gcataggtac taacctgttt atgtcctgtt atttacaggc atagcattat tggttaatta
       61 attcattcga ctctatatta ttaccttttt ctttttcctt tttaatttta gcctctacca
      121 tatcgaacat tttcattgtc tgtgatgaag ttagctcttt cttttgtcgt agcgcgttac
      181 gtatggtaga agcttcaata tcagaaactg ttaaaagttc ttctacccta ctcttcaatt
      241 gctgtaatga atggctatta tcaactttaa agaaatcatc cattacctca agtgaggcaa
      301 caatcaaatc ctgtagtctc tctttttcta tctcgatatt ttcaatggtt tgtttttctt
      361 tagatactaa ctgctttaaa ttattaattt ctttctgaat cagtttcttc tctgtctgta
      421 gctcttctgt gaccagctct tcacgcttac cacgctctac ttccgcttta atatcagcat
      481 caattttagc attttctttc tcttcttctc tggcagcccg aacctttaac cccgttaacc
      541 tgattgaatc caagtattct ttttcctttg taagataaac attttcattg tgtttatctg
      601 tagcccattt ttgagcaaga gaaagtaacc attgagcata aggagtaata acagctagca
      661 cacctccaaa aaagacagga gcaagcagat aatgagcgta aaaatattcc tgagatagta
      721 tataatctat tcgaaactta acaggcgcat cactcataaa gagcattgct aagttaggcc
      781 agttgaacca tacccatgca agaatgatat atccccataa tgggctttta atcctatcgt
      841 ttattgcttg catagtagaa tctaaccacc catcactcat agctaaccct cggaagtctt
      901 atctttctgt ctatcataac cacaagcatc acctttagga accatctgaa actggaggtg
      961 tatttagacc ttgctaatct tagacagccg cccggttcat gcagcacgtc cacacagtga
     1021 agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg tactgacggc
     1081 gaaaaatcca ctgatccgtt ttatcggtag tgatgatatg acgcaaaacc gggagttatt
     1141 tcaggtctgg ttacaaaaat tagcacagtg gcatcagacc actacgcctt atcttttttt
     1201 acatacgcca gatattgccc aggccccgga actggtacat accctgtggg aagacttacg
     1261 taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt ctcttttctg
     1321 aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt tgtatggtaa
     1381 gcgcgctgta tgccgtttta agtgcgttgt tattaatgaa gttctctttt gatgtcgttc
     1441 gcctgcgaat gcagtaccgc gttgcctatg gcgacggcgg ttttagcgaa ctgcaaagcg
     1501 ctattcgcat tcatggtaac gcggtggaat atattcctat cgcgattgtg ttgatgctgt
     1561 ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc gttttgcttg
     1621 ctggtcgtct gatgcattat tacggttttc atcaccgtct gttccgctgg cgacgttctg
     1681 gcatgagcgc gacctggtgt gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc
     1741 cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt tttcccggat
     1801 ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag actgggcgac
     1861 tggacctttg atgaacgggt agctgaagtc ttcccggata tgatccagcg ttccgttccc
     1921 ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt tcaacctggt
     1981 acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac
     2041 attcatcatg ataattgcaa aattattgcc atcgataact ccccggcgat gattgaacgc
     2101 tgccgtcgtc atattgacgc ctataaagct cctacgccag tagacgttat tgaaggtgat
     2161 attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac cctgcaattc
     2221 ctggaacctt ccgagcgcca ggcgttactg gataaaattt atcaagggct gaacccgggc
     2281 ggtgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg tgaattgctg
     2341 ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga gatcagccag
     2401 aaacgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca taaagcacgc
     2461 ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa ctttggttca
     2521 ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt tattctctga
     2581 ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt gctaactggc
     2641 agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cgcggtggaa tttctgcctg
     2701 aaattaaacc gtatcgtctt gatttactgc atagcgtgac ggcagaaagc gaagaaccac
     2761 tgagcgccgg gcaaattaaa cgcattgaaa cgctgatgcg caacctgatg ccgtggcgca
     2821 aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc gactggaaat
     2881 gggatcgcgt tctgccccat ctttctgatt taaccgggcg caccattctt gatgttggct
     2941 gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc gcggtgggga
     3001 tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta ctgggcaacg
     3061 atcagcgcgc acatttgtta ccgttaggta ttgaacaact tccggcactg aaagcctttg
     3121 ataccgtctt ttcgatgggc gtgctttatc atcgtcgttc accgctggag catctctggc
     3181 agttaaaaga ccaactggtg aatgaaggcg aactggtgct ggaaacgctg gttattgatg
     3241 gcgatgagaa cacggtgctg gtgccgggcg atcgttacgc acaaatgcgt aatgtctatt
     3301 tcattccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt gttgatattc
     3361 gcattgcaga tgtgagcgtt accaccacag aagagcagcg acgcaccgaa tggatggtca
     3421 ccgagtctct ggccgatttt ctcgacccgc atgatccggg taagacggtg gaaggttatc
     3481 ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt aatactgccg
     3541 gatgcggcgt gaacgcctta tccggcctac aaagtcttgc taattcaata tattgcaggg
     3601 gctatgtagg cctgataagc atagcgcatc aggcagtttt gcgtttgcat aacctcagcg
     3661 cccgtttccg ggcgcttttc acgtcttatg cctgaacagc tggctgatca aacgccgtta
     3721 acttcagcgc attgcccgta tatttttcga tatacaccag cgcagagtta ccggcacagg
     3781 cattcgccag ctgcgagctg gggatatccg ccgttaacac gttcgcacta ccgtttttac
     3841 acaagccatt ttccagatct ggccatgcac cttcatgcac gcataccacg ccttttttga
     3901 tcccgtcagt caccaccgcg cctgtcagga tctgaccgcg cttgttccag acacgcacca
     3961 gatcgccatt cgcaatacca aagcgagcag catcttcggt gtgaatagtg attggttcac
     4021 gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg tgtaaacggt
     4081 gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc ttccactcat
     4141 caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt tccagcgttc
     4201 tggagtaaat ctcaattttg ccgcttggcg tacccagcgc atttttcacc ggatcggcgc
     4261 ggaaatcacc ataacgaatg tactgttcgt tcttctcgct gcggcgcatt tcgatcagtt
     4321 tattttgctg ccagaaggca ttaaacatcg gcatagtcac gcgttgcgca cgggcacctt
     4381 tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct tcggtataga
     4441 tctcttttcc gccaggtttg agtaattcag caagatcggc aaacacgtca aaatcgttgc
     4501 gcgcttcaaa ttgcgcagcg acagcctgct tcatcggcac aatatgctgg ttgctgtaat
     4561 caccggtcat cgtcaggtca ttgcgctcaa acgatgtggt gatcggtaat acgatatctg
     4621 cgtgtttggc tgctgcggtc cagtagcatt cagaaacgac gatcatctcc ggtttctgcc
     4681 atgctttaat cagacgatta gtatcctggt gatgggtaaa gttaccgcca cccgcccacc
     4741 agatcatttt gatattcgga tacgtttgtt ctttaccgtt atgctgatat ttgctgccag
     4801 gattttccag cgcatcgacg atacgggcaa caggaatcgc cgtcattccg ccatcatcag
     4861 cggcctcgct ggcctgtccg gcaatcgccg cagacatttc tggtagcacg ccgccaacac
     4921 gcgtcgggtt gccaccgttg gagtaatgat aagagaaacc aaacccgccg cccggtgtac
     4981 caatctgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt tcaccatatt
     5041 gctggcgctg aattccccag cctgccatca gcattgtgcg atttgccgcc atcagttcag
     5101 cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgcccag gccgcgctct
     5161 tcggcgtatt atcgctctta cctgtcagat actcttcaaa ctgcggatac ccggtagtgt
     5221 atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc gcaatcctaa
     5281 catcagtgcc acatcggtgc ccatattcgg tgcgatccag gtggcattat cgccaaagaa
     5341 ttcgatggtt tcggagcaga taggatcaat ggcaatcact ggtttgccag attttttcag
     5401 ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt tgagcgtgtt
     5461 cagcgggttc atcccccaca gcaccaccac ctggctgttt tccagaatca gcggccagga
     5521 ggtctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca cctgtgccgc
     5581 tccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca tataacgttg
     5641 cagtaaggtt tgcgctttat gcaacacgcc agaagaacgc cagccataag atccggcaaa
     5701 aatggccgat ggtccgttag ctttgcgaat gcgatcgtgt tgttcatgaa tcagttttaa
     5761 tgcttgttcc cagctcacct gtacataggt atcttcgcca cgacctttca ccggttgcag
     5821 tggattatcg agatagcttt ttctcaccat cggatgctga atacgcgccg tggtgtgtac
     5881 ctgatccgcc gccgtagact gtaatgagtt cggtatggtt ttcgccagcg cgcctgtcga
     5941 agaaacaatc ttgccgtcct tcacttctac gttcatcgct ccccaacggc ccgcggtgag
     6001 aattttgccg cccttttctt ctgcccatgc gggtagcggt gcagccgatg tcaccaccag
     6061 cgtccctgcc gcaataccgc tatgtttaat aaattcacgt cttgttaatg tcataacttc
     6121 ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt actggaagta
     6181 ccgcgtcaaa atgtccagtt cgttttcgct catgctggtt cgcgccccca ttcctttggc
     6241 aatggacggc catgtattga cggtgtaatg gtcggcggca ataggggcat ggcagccagc
     6301 gcaataggta ccgtcaagtt tttcagcgta ttgccatagc ggtttacggt ccgctaatgc
     6361 gggatcgata agcgccccct gcaaagacgc ctgacgccat tgattgccgt attcgtcagc
     6421 ctgccattcc ccgtttaccg ttagcgcctt gataccttct tcacttaatg tggcgagcgc
     6481 cagccgttgg cctaccgcca ggtagagcgt gttttcactg ccctgcattt gataaccctg
     6541 caaccgaaca attggctgtt tgccactggc atcaacgacg gtaagatcgg taccaggagt
     6601 cacggtagcc agttcgccta tgcgagaagt tttgaaagga taaatatgtg cgccattagt
     6661 aactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca tttttatttc
     6721 tggcggaaaa tgggcaatgc ctttatggca atcgatacag gtttcgccgt ccttttgcgc
     6781 tttattatgc attttctgcg cagattcact ttgcgaggca atatccatgg catcaaaaga
     6841 atggcaacta cggcaggttg cagagtcagt tgcttttaat tctttccata ctgtttcggc
     6901 catttcctgg cgatgagctt cgaacttatc atcactgtct attttgccgc taacaaattc
     6961 atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc ctgactttgg
     7021 aatatggcaa tcggcacatt ccgcacgtat ccctttctgg tttgaaaagt ggacagttcc
     7081 ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg cggtatccga
     7141 cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca caaccgctat
     7201 cagcaaaaac aataacccaa tgcgtttttt ccctcgcata gattcctcta attaaattcc
     7261 aggacaccga taccattgta taaataaata cacaacgaat tgactacttg cttaatt
//
LOCUS       DAZFRG010000227         7262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFRG010000227 DAZFRG010000000
VERSION     DAZFRG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.228"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..262)
                     /locus_tag="ACHKUC_003990"
                     /pseudo
     CDS             complement(176..262)
                     /locus_tag="ACHKUC_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            304..534
                     /locus_tag="ACHKUC_003991"
                     /pseudo
     CDS             304..534
                     /locus_tag="ACHKUC_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            586..1521
                     /gene="ttcA"
                     /locus_tag="ACHKUC_003992"
     CDS             586..1521
                     /gene="ttcA"
                     /locus_tag="ACHKUC_003992"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO6620160.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1650..3023)
                     /gene="dbpA"
                     /locus_tag="ACHKUC_003993"
     CDS             complement(1650..3023)
                     /gene="dbpA"
                     /locus_tag="ACHKUC_003993"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO6620161.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVSLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3053..3226)
                     /gene="ynaL"
                     /locus_tag="ACHKUC_003994"
     CDS             complement(3053..3226)
                     /gene="ynaL"
                     /locus_tag="ACHKUC_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO6620162.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3501..4484)
                     /gene="zntB"
                     /locus_tag="ACHKUC_003995"
     CDS             complement(3501..4484)
                     /gene="zntB"
                     /locus_tag="ACHKUC_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO6620163.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4498..4545)
                     /gene="ydaN"
                     /locus_tag="ACHKUC_003996"
     CDS             complement(4498..4545)
                     /gene="ydaN"
                     /locus_tag="ACHKUC_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO6620164.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4739..5971
                     /gene="dgcM"
                     /locus_tag="ACHKUC_003997"
     CDS             4739..5971
                     /gene="dgcM"
                     /locus_tag="ACHKUC_003997"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO6620165.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQI
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5992..6555)
                     /gene="smrA"
                     /locus_tag="ACHKUC_003998"
     CDS             complement(5992..6555)
                     /gene="smrA"
                     /locus_tag="ACHKUC_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO6620166.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6750..>7262)
                     /locus_tag="ACHKUC_003999"
     CDS             complement(6750..>7262)
                     /locus_tag="ACHKUC_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620167.1"
                     /translation="GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVT
                     LTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHT
                     VNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAF
                     LLVPAMNKSS"
BASE COUNT         1940 a         1852 c         1688 g         1782 t
ORIGIN      
        1 cgcaaatata accaccaacc atacccatca tttattacag agaattattt tattcaccga
       61 aaagtttcac aaaatatccc ctgatagaaa actaaaaaac actgcataat atcgctattc
      121 tggcacttgc gaaaaattat attctgcact tgttgtagcg ttctatttct ccgcctggca
      181 acgaatgcac aatgtccgcc acagtttctg ttctaatttc ttcctgtgtt accgcatcct
      241 gtacgggtag cgcaacacca gcatgctaag caaggctttc ccatcagatt tcactacgac
      301 tatttgggag cgagatggaa tgcagcagta aaacgtgctg gtattcgccg ccgtaatccg
      361 taccatacgc gacatacttt tgcctgctgg ctgttgacgg caggagcgaa cccggcattt
      421 atagccagcc agatggggca tgaaactgcg cagatggtgt atgaaattta cggtatgtgg
      481 attgatgaca tgaacgacga acaggtagcc atgttgaatg cgcggttatc gtagttgcaa
      541 agtttgccct caatatgcca catttagtac cagagaactg aaataatgca agaaaatcaa
      601 caaattacaa agaaagaaca atacaacctg aacaaattac aaaaacgtct gcgtcgtaac
      661 gtgggcgaag ccattgctga cttcaatatg attgaagaag gcgatcgcat catggtttgc
      721 ctctccgggg gtaaagacag ctataccatg ctggagattc tgcgcaattt gcagcaaagc
      781 gcgccaatca atttttcgct ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg
      841 gaacacgttc tgcccgagta tcttgaaacg ctgggcgttg agtacaagat tgttgaagag
      901 aacacttacg gtatcgtgaa agagaagatt ccggagggca aaaccacttg ctcactgtgt
      961 tctcgtcttc gtcgcggtat cctttatcgc accgcaacgg aactgggggc gacgaagatc
     1021 gcgctgggtc accatcgtga cgatatcctg caaacgctgt tcttaaatat gttctacggt
     1081 ggcaagatga aaggtatgcc tccgaaactg atgagcgatg atggcaaaca tatcgtgatt
     1141 cgtccgctgg cctactgccg cgagaaagat attcagcgct ttgccgatgc aaaagcgttc
     1201 ccgattattc cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca ggtgattgct
     1261 gacatgttgc gtgactggga taaacgttat ccggggcgta tcgagacgat gttcagcgcg
     1321 atgcagaatg tggtgccgtc gcatctgtgc gataccaacc tgttcgattt caaaggcatt
     1381 acccacggtt ctgaagtggt taacgggggt gatctggcgt ttgatcgcga agagatccca
     1441 ctacaaccag cgggctggca gcctgaagaa gatgaaaatc agttggatga gttacggctg
     1501 aatgtggttg aagtgaaata accaggatag cgcccgatgc gcaagcgtat cgggctactc
     1561 ttatggaggc cggagaagac gcggccagcg tcacatccgg caatcccgaa gaagatgttt
     1621 actcttgcac cctgcaattc aacatttcat tattttaata accgcacccg gcacgttttt
     1681 cctttaatct tcccgccctg tagctgtttc catgctttat gagcaacagc ctgacgcacc
     1741 gcgacataaa catgcgccgg atgcacggcg attttgccaa tatctgcgcc atcaagcccg
     1801 atgtctcctg tcagcgcacc taatacatca cccgggcgca ttttggcttt tttcccgcca
     1861 tcgatacaca acgttgccat ttctgcttcc agcggcacaa tggaactatt cgctggcggc
     1921 gtttgccagt taagttttat ctgcaacatg tcagaaatga tattggcccg ctgtgcttct
     1981 tccggggcac agaaactgat cgccagaccg ctatttcctg cacgagctgt acgaccgatg
     2041 cgatgtacat gaacttcagg gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat
     2101 tttatatcca gaccacgcgc agcaacatca gtcgcgacca atacacgggc gctaccgtta
     2161 gcaaaacgta ccagggtctg atcgcgatcg cgttgctcca gatcgccgtg taacgacaat
     2221 gcactttgcc ctacttcatt cagcgcgtcg cagacagact ggcaatcttt tttggtattg
     2281 caaaacacca cgcaagagga tggctgatgc aagcttaata accgttgcaa cagaggaatt
     2341 ttgcctttgc tggatgtctc ataaaattgt tgttcaatag gtggcagagc atctgttgag
     2401 tcaatttcaa tcgctaaagg atcgcgttgc actcttccgc tgattgcggc aatggcttcc
     2461 ggccaggttg ccgaaaacag aagtgtctgc cgagatgctg gcgcaaaaca gatgacatca
     2521 tcaatggcgt cgctaaatcc catatccagc atgcggtcgg cctcatccat caccagcgta
     2581 ttcaacgcat ccagtgatac agtgcctttt tgcaggtgat ccagcaaacg gcccggcgtt
     2641 gccacgataa tatgcggcgc atgttgcaac gaatcacgct gcataccgaa cggttgacca
     2701 ccgcacaacg tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg caattcacct
     2761 gccacctgat ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc
     2821 gacacatcaa tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt
     2881 ttcgcctgca cgcgaacatc ttttccggca aggatcgccg gaagcgcggc ggcctgcacc
     2941 ggcgtcatgg ttaaataacc caactcatta aggtttgtga gttgggcggg aggcaaaaca
     3001 ttcagggtag aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa agtcagcagg
     3061 cgcgtatcct cgcagatcta cgctcacgat gcgacaattt aatcggttct tcatcgggtg
     3121 gtgggtcagg catgggttgc gggcgaggga tcggatcggg tactggaaca ggatcgccag
     3181 gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc
     3241 attgggtgga ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca
     3301 tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga
     3361 caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaagag agatattgcc
     3421 ctgaatgggt agagagttta ttgacttcgc tcaaacttta cggcgttttt gtatacagac
     3481 agccggaaaa attgcttttg ttacaaccat ttactacgat gcaaccataa agcaacacca
     3541 ccaataagaa caactaacag aatacaaaaa attgaaaatc caaattgcca tccgccgcca
     3601 gggatcccac caaggttgac gccaaataac cctgtcagaa acgtactggg taaaaagacc
     3661 attgccatca acgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg
     3721 atttcatccg ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aagaccgcgc
     3781 ccaaggcgat cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa
     3841 cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcaca
     3901 attaattgtt tgcgcagcag agccagaaat ccacgcggtg gaatttgctg atcgagaaga
     3961 ttatcttcga ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg
     4021 gtcaacgcat cgcacacatc caccagccac cccccgcaat cggtcggacc agtgccctct
     4081 tcaagatcgc tcaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt
     4141 aaccgcccgt ccatatatac acgcatagcg accagttgat cggggcgttc atcggtgctg
     4201 ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga gacggctgac tcggggccgt
     4261 gtgctctcgc ccgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc
     4321 cattgggcgc tatcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca
     4381 atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat
     4441 actgcatccg ggacattaac gtccgatccc ttaatcgctt ccacagtgcc tccatcatca
     4501 acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta
     4561 ccctgaaatg attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg
     4621 tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat
     4681 gacgtggctt ttttgcgatc ggatagcaac aaaaattgat aaaaataatg ggatctcaat
     4741 gattacacac aacttcaata ccctggactt actcaccagt cctgtctgga tcgtttcgcc
     4801 ctttgaggaa cagttaattt atgccaacag cgcggcgcga ctgttgatgc aagacctcac
     4861 gtttagtcag ctacgaaccg gtccctattc cgtctcctca caaaaagaac tgccgaaata
     4921 cctctccgat ctgcaaaatc aacacgatat tatcgaaatc ctcaccgttc agcgtaaaga
     4981 agaggaaaca gcattaagct gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt
     5041 gattattttc gaaggtatcg aagcaccggc aacgctgggt ttaaaagcca gtcgctcggc
     5101 aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc
     5161 aatgttgttg attgacccct cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct
     5221 caatttctat ggttataacc atgaaacgat gtgccagaaa catacctggg aaattaatat
     5281 gctcgggcgt cgcgtcatgc ctatcatgca tgaaatctcg catttacccg gtggtcataa
     5341 acctttgaat tttgttcata aactggcgga tggttcgact cgtcatgtgc agacctatgc
     5401 cgggccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga
     5461 gcaaaaacgg ctggaggagc agctggaaca tgctgcccac catgatgcga tgaccggatt
     5521 actgaatcgg cgacagtttt atcacattac cgagccaggc caaatacagc acctcgccat
     5581 cgctcaggat tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct
     5641 ctatgggcat tctaaaggtg atgaggtgtt atgcgccctt gcccgcactc tcgaaagttg
     5701 cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc
     5761 aagaacccca ctggataccg cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa
     5821 agtgagtatt tcgggcttac cacgctttac tgtcagcatt ggtgtggcgc atcacgaagg
     5881 aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gagcgaaaaa
     5941 tgatggacgc aaccgcgtgc tggcggcata agccgcggat gcgtctcgtg atcaacgact
     6001 gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gctgttttac gtagtgcgac
     6061 gtaacacgcc ccgctgccgc catgatgcgg tagcgcggtg caatatgcct gaacatcatc
     6121 aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcatc
     6181 ccgtcctttg ccatgaataa tcagcacgtt acgcaaacca tccgccaggg cttgttgaat
     6241 gaaactgaac atcattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc
     6301 ctgttgcgga tatttaccac tgcgcagctt atccagcacc ccatgttgca acccttcccg
     6361 ccgaaactcc agcggctgac ttagcgggat gatgtcgaga aatccagtgg tgaggaaatt
     6421 atcaagttgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg
     6481 gacatctgta gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt
     6541 gtcgtcaagg ttcatgtaac ctcctatgca ccttcacacg tcgtgattca gacgggttaa
     6601 ttgttcatac ttactgcatc gttcccttca cagattgaaa caatactcta cttcatacct
     6661 gtgagtatct gcgcaggcgg ggcattttat actagcctgt tagcagtatc gaacagttta
     6721 gcccgagttt aattcagcac aggctccgat caggatgatt tattcatagc aggaaccaac
     6781 aaaaatgcga taaataacag accactgatt agcagaaagg tatccgaaaa tgccatagtt
     6841 tgcgcctccc tcagcatcaa ttgactcaat aatttggttg aagccagtat ttccgatgtc
     6901 tggtcacttc cactgcgatt aaaaaacaaa gcacttctgg aaatgaagtc attcaccgta
     6961 tggggaacag aaaccatttt ttcacccatt ctcgaaaaat gaaaattagt tcgattattg
     7021 agaatagagc cacacagggc aataccaatt gcgcccccaa gattacgtgt taaattaaat
     7081 acaccagagg ctaacttcag ccgttctttt ggaattcccc ccagcgttaa tgttacgatg
     7141 ggggccatcg caaactgctg cgaaatccct ctaattgcct gtggaaacag taactcctgc
     7201 catccccatt cgtgggttat aggggtaaaa agatacattg aaaacacaaa tccgcccagc
     7261 cc
//
LOCUS       DAZFRG010000228         7217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFRG010000228 DAZFRG010000000
VERSION     DAZFRG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.229"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..525
                     /gene="sufA"
                     /locus_tag="ACHKUC_004000"
     CDS             157..525
                     /gene="sufA"
                     /locus_tag="ACHKUC_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO6620168.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            534..2021
                     /gene="sufB"
                     /locus_tag="ACHKUC_004001"
     CDS             534..2021
                     /gene="sufB"
                     /locus_tag="ACHKUC_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO6620169.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            2031..2777
                     /gene="sufC"
                     /locus_tag="ACHKUC_004002"
     CDS             2031..2777
                     /gene="sufC"
                     /locus_tag="ACHKUC_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO6620170.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            2752..4023
                     /gene="sufD"
                     /locus_tag="ACHKUC_004003"
     CDS             2752..4023
                     /gene="sufD"
                     /locus_tag="ACHKUC_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO6620171.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            4020..5240
                     /gene="sufS"
                     /locus_tag="ACHKUC_004004"
     CDS             4020..5240
                     /gene="sufS"
                     /locus_tag="ACHKUC_004004"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO6620172.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPLDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            5253..5669
                     /gene="sufE"
                     /locus_tag="ACHKUC_004005"
     CDS             5253..5669
                     /gene="sufE"
                     /locus_tag="ACHKUC_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO6620173.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            5818..6822
                     /gene="ldtE"
                     /locus_tag="ACHKUC_004006"
     CDS             5818..6822
                     /gene="ldtE"
                     /locus_tag="ACHKUC_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO6620174.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGEKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(6886..7122)
                     /gene="lpp"
                     /locus_tag="ACHKUC_004007"
     CDS             complement(6886..7122)
                     /gene="lpp"
                     /locus_tag="ACHKUC_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO6620175.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         1823 a         1751 c         1972 g         1671 t
ORIGIN      
        1 agcgtgcctg ttaacccaca catcagggtc tatgcttatt aaaagcagga aaaaagcatg
       61 ttttacgcta aagcccctgc gtttgctggg ttgaactgat aatcattatc actaacatgc
      121 tgttatacgc tgaaagcgat gaagtgaggt aaatcgatgg acatgcattc aggaaccttt
      181 aatccacagg atttcgcctg gcagggctta acgctgacac ccgcagccgc tgtccacatc
      241 cgcgagctgg tggcaaagca gccgggtatg atcggcgtgc gcttaggcgt gaagcaaaca
      301 ggctgcgcgg gctttggcta tgtgctcgat agtgttagcg agccggacaa agacgatctg
      361 ctgtttgaac acgacggcgc gaagctgttt gtcccgctgc aagcgatgcc ttttattgat
      421 ggcacggaag tcgatttcgt tcgcgaagga cttaatcaga tattcaaatt tcacaaccct
      481 aaagcccaga atgaatgtgg ctgtggcgaa agctttgggg tataggcggt actatgtctc
      541 gtaatactga agcaactgac gatgtcaaaa cctggaccgg cggcccgctg aattataaag
      601 aaggattctt cacccagtta gccaccgatg agctggcaaa ggggataaac gaagaggtgg
      661 tgcgcgcaat ttcggcgaag cgtaatgagc cggagtggat gctggagttt cgtctcaacg
      721 cctatcacgc atggctggag atggaagaac cgcactggtt gaaagcgcac tacgacaagc
      781 tgaattatca ggattacagc tactactcgg caccatcgtg cggtaattgt gacgacaact
      841 gcgcgtctga acccggcgcg gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg
      901 tggaggcggc gtttgagcag ttgggcgttc ccgtgcggga aggcaaagag gtggcagtgg
      961 atgccatttt cgactctgtt tcggttgcca ctacttatcg cgaaaaactg gcggagcagg
     1021 gaattatttt ttgttccttt ggcgaggcga tccacgatca cccggaactg gtgcgtaaat
     1081 atctcggcac cgtggtgccg gggaatgaca acttctttgc cgcacttaat gcggcggtag
     1141 cctctgacgg tacgtttatt tatgtgccta aaggcgtgcg ttgcccgatg gaactttcca
     1201 cctattttcg cattaacgcg gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg
     1261 acgaagacag ctacgttagc tacattgaag gctgttccgc tccggtgcgt gacagctatc
     1321 agttacacgc ggcggtggtg gaagtcatca tccataaaaa cgccgaggtg aaatattcca
     1381 cggtacaaaa ctggttccct ggcgataaca acaccggcgg tattctcaac ttcgtcacca
     1441 agcgtgcttt gtgcgaaggc gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt
     1501 cagcgattac gtggaaatat cccagctgca ttttgcgcgg cgataactcc attggtgagt
     1561 tttactcagt ggcgctgacc agcggtcatc agcaagcgga taccggcacc aagatgatcc
     1621 acatcggtaa aaacaccaaa tcgaccatta tatcgaaagg gatctctgcc ggacatagtc
     1681 agaacagtta tcgcggctta gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca
     1741 ctcagtgcga ctcaatgctg attggcgcta attgtggggc gcataccttc ccgtatgtcg
     1801 aatgtcgcaa taacagcgca caactggagc acgaggcaac gacatcacgt attggtgaag
     1861 atcaactgtt ttactgcctg caacgcggga tcagcgaaga agacgccatc tcgatgattg
     1921 ttaacggttt ctgcaaagac gtgttctcag aactgccgct ggaatttgcc gttgaagcac
     1981 aaaaactcct cgccatcagt cttgaacaca gcgtcggata aggaataaac atgttaagta
     2041 ttaaagattt acacgtcagc gtggaagata aagctatcct gcgtggatta agcctcgacg
     2101 ttcgtcccgg cgaagttcac gccattatgg ggccaaacgg ttcgggcaaa agtaccttat
     2161 cggcaacgct tgccgggcga gaagattatg aagtgacgag cggcacggtt gagtttaaag
     2221 gcaaagattt gcttgcgctg tcgccggaag atcgtgcagg tgaaggcatc tttatggcct
     2281 tccagtatcc ggtggaaatt cccggcgtca gtaaccagtt tttcctgcaa acggcactta
     2341 atgcggtgcg cagctatcgc ggccaggaaa cgctcgaccg ctttgatttt caggatttga
     2401 tggaagagaa aatcgctctc ctgaagatgc cggaagattt attaacccgt tcggtaaacg
     2461 ttggtttttc cggcggcgag aaaaagcgca acgatatttt gcaaatggcg gtgctggaac
     2521 cggagttatg cattcttgat gagtcagact ccgggctgga tattgacgca ttaaaagtgg
     2581 ttgccgatgg cgtgaactcg ctgcgtgacg gcaagcgttc attcatcatt gttacgcact
     2641 accaacgcat tctcgactac atcaagcctg attacgtcca tgtgctgtat cagggacgaa
     2701 ttgtgaaatc cggcgatttc acgttggtca aacaactgga ggagcagggt tatggctggc
     2761 ttaccgaaca gcagtaacgc actgcaacag tggcatcatt tgtttgaagc tgaaggggcg
     2821 aaacgctccc cgcaagcaca gcagcattta caacaattgc tgcgtaccgg actgccgaca
     2881 cgtaaacatg aaaactggaa atatacgccg ctggacgggc tgaccaatag ccagtttgtc
     2941 agcattgcgg gggagatatc cccacagcag cgtgatgcct tagcgttaac attagacgcc
     3001 gtgcggctgg tgtttgtcga tggacgttac gtgcccgcac tgagcgatgc aactgaaggc
     3061 agcggatatg aagtgagcat taacgacgac cgtcaggggt tacccgacgc tattcaggcg
     3121 gaagtgtttc tgcatttgac ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag
     3181 cgcggtcaac ggccggcaaa gccattgctg ttaatgcata tcacccaagg cgtggcaggt
     3241 gaagaggtga atactgccca ttaccggcat catctggatc tggcggaagg tgccgaagca
     3301 acggtgatcg aacattttgt cagccttaat gatgctcgtc acttcaccgg cgcacggttc
     3361 actatcaacg tcgcagcgaa cgcccatttg cagcatatca agctggcgtt tgaaaacccg
     3421 ctcagtcacc actttgctca taacgatttg ttgctggctg atgatgccac cgcatttagc
     3481 cacagtttcc tgctgggtgg cgcagtgtta cgacacaaca ccagtacgca actcaatggc
     3541 gaaaacagca cggtgcggat caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat
     3601 actcgtacct ggctggaaca caataaaggt ttttgtaaca gccgacagtt gcacaaaact
     3661 atcgtcagcg acaaaggccg cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc
     3721 atcaaaacgg atggtcagat gaccaacaat aatctgctga tgggcaaact ggcggaagtg
     3781 gatactaaac cgcagctgga aatctatgca gatgatgtga aatgcagcca cggcgcgacg
     3841 gtggggcgta ttgatgacga acagatgttt tatctgcgct cgcgcgggat caatcagcaa
     3901 gatgcccagc agatgatcat ttacgccttt gctgccgaac tgacggaagc actgcgtgat
     3961 gaggggctta aacagcaggt gctggcccga atcggtcaac gactgccagg aggtgcaaga
     4021 tgactttttc cgtcgacaaa gtgcgggccg actttccggt gctttctcgt gaggtaaacg
     4081 gtttgccgct ggcttatctc gacagtgccg ccagtgcgca gaaaccaagc caggtgattg
     4141 acgccgaggc tgagttttat cgtcacggct acgcggcggt gcatcgcggt attcatacct
     4201 taagcgccca ggcgaccgag aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta
     4261 atgcccgttc ggcggaagaa ctggtgttcg tccgcggcac gacggaaggg atcaatctgg
     4321 tcgccaatag ctggggcaac agcaatgtgc gggcgggcga taacatcatc atcagtcaga
     4381 tggagcacca cgctaacatt gtcccctggc agatgctttg tgcacgcgtt ggcgcagagc
     4441 tgcgtgtgat cccgctcaac cccgacggta ctctgcaact ggagacgctg cctacgctgt
     4501 ttgatgagaa aactcgcctg ctggcaatta ctcatgtctc caacgtgctt gggacagaaa
     4561 atccgctggc ggagatgatc acacttgcgc atcaacatgg cgcaaaagtg ctggtggatg
     4621 gcgctcaggc ggtgatgcat catccgctgg atgttcaggc gctggattgc gatttttatg
     4681 tgttctccgg gcataaactg tatggcccta ccggaattgg cattctttat gtcaaagaag
     4741 ccttgttgca ggagatgccg ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc
     4801 tgagtgaagg cactacctgg accaaagcac catggcggtt tgaagccggt acacccaata
     4861 ccggaggtat cattggtctt ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata
     4921 acatagccga gtatgaacag aatctgatgc attacgcgct atcacagttg gaatctgtac
     4981 cggatctcac tctctatggc ccacaaaaca ggcttggcgt tattgctttt aatctcggta
     5041 aacaccacgc ctatgatgtt ggcagttttc tcgataatta cggcattgct gtgcgtaccg
     5101 gacatcactg cgcaatgcca ttaatggcct attacaacgt ccctgcgatg tgtcgggcat
     5161 cgctggccat gtataacacc catgaagaag tggatcgtct ggtgaccggc ctgcaacgta
     5221 ttcaccgtct gctgggataa cagggaggca ctatggcttt attgccggat aaagaaaaat
     5281 tgctgcgtaa ttttttacgc tgcgccaact gggaagagaa atatctctac attattgagt
     5341 tgggtcagcg tctgccagaa ttacgtgacg aagacaaaag cccgcaaaat agcatccagg
     5401 gctgccagag tcaggtgtgg attgtgatgc gtcaaaatgc gcagggaatt attgaattac
     5461 agggcgacag cgatgcggcc attgtaaaag ggcttattgc ggtcgtcttt attctttacg
     5521 atcaaatgac gccgcaggat attgtcaatt tcgatgtgcg cccgtggttt gaaaaaatgg
     5581 cgctcaccca acatctcacc ccatcacgct cacaaggtct ggaagcgatg attcgcgcaa
     5641 ttcgcgccaa agccgctgca cttagctaaa ctaaagggac agctttcatc cggttggctc
     5701 acattgccgg gtggtcaatg tttacactgg cctgcacagg agtgttttgg tttcaggtga
     5761 acataagcgc catccgacgt ttcagcgtga gtctccggca aatacaggag gtttacaatg
     5821 aaacgcgcgt ctttgcttac actcacgctt atcggcgctt ttagcgccat ccaggctgcc
     5881 tgggcggttg attatccgct accaccaacc ggcagtcgac tggttgggca aaatcaaacg
     5941 tatacggtgc aagaagggga aaaaaacctt caggccatcg cccgacgttt tgatactgcg
     6001 gcaatgttga tccttgaagc caataacact atcgccccgg tgccaaaacc cggtacgacg
     6061 ataactattc cttcacaact attattacct gatgcgccgc gtcaggggat tattgtaaac
     6121 cttgcagagc tgcgccttta ttattatccg ccgggagaaa atatcgtgca ggtttatccg
     6181 ataggtattg gtttgcaggg gctggaaacg ccggtgatgg aaacgcgtgt ggggcagaaa
     6241 atccctaacc caacctggac gcctacggcg ggcattcgtc aacgttcgct ggagcgtggc
     6301 attaaattac caccagtcgt tcctgccggg cccaataacc cactaggacg ttacgcactg
     6361 cgcctcgcgc atggtaatgg cgaatacctc attcatggca ccagtgcgcc ggacagcgtc
     6421 ggtttgcgcg tcagttcggg ctgtattcgc atgaatgcac cggatattaa agctttattc
     6481 tccagcgtgc ggacgggaac accggtgaaa gtgatcaacg aaccggtgaa atattccgtg
     6541 gaacctaacg ggatgcgtta tgttgaagta catcgaccac tatcggcaga agaacagcag
     6601 aacgttcaga caatgccgta cacgctgcca gcaggcttta cgcaatttaa agacaataag
     6661 gctgtagatc agaagttagt cgataaagcg ttgtatcgtc gggcagggta tccggttgcg
     6721 gtgagcagtg gagcaactcc cgcagccagc aatgcgcctt cagtagagtc agcgcagaat
     6781 ggggaaccag agcaagggaa tatgttacgc gcgacgcagt agcggtaaat agcaggcaaa
     6841 aaaaatggcg cacaatgtgc gccatttttc acttcacagg tactattact tgcggtattt
     6901 agtagccatg ttgtccagac gctggttagc acgagctgcg tcatctttag cagcctgaac
     6961 gtcggaacgc attgcgttca cgtcgttgct cagctggtca actttagcgt tcagagtctg
     7021 aacgtcagaa gacagctgat cgattttagc gttgctggag caacctgcca gcagagtaga
     7081 acccaggatt accgcgccca gtaccagttt agtagctttc attattaata ccctctagat
     7141 tgagttaatc tccatgtagc gttacaagta ttacacaaac ttttttatgt tgagaatatt
     7201 tttttgatgg gaaggca
//
LOCUS       DAZFRG010000229         7173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFRG010000229 DAZFRG010000000
VERSION     DAZFRG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.230"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            153..743
                     /locus_tag="ACHKUC_004008"
     CDS             153..743
                     /locus_tag="ACHKUC_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6620176.1"
                     /translation="MSKKLGFVLSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIDGSAIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            852..1592
                     /locus_tag="ACHKUC_004009"
     CDS             852..1592
                     /locus_tag="ACHKUC_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6620177.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKNVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1627..4215
                     /locus_tag="ACHKUC_004010"
     CDS             1627..4215
                     /locus_tag="ACHKUC_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO6620178.1"
                     /translation="MTIKSTNHLTHIATFCALLYSNSALCAELVEYDHTFLMGKDASN
                     IDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFAVIEGKKNAQACITQKNLLQFHI
                     SSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASGNYNW
                     MTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRMLPPV
                     LASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVTIEEA
                     DGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNNYLTG
                     YTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWNKLFE
                     NTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVTVSIN
                     QPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLNEDGQ
                     TDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTNRISY
                     SVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFVLHSG
                     GLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRIALDI
                     NDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAILNIVRSDGKNIPFAADIYDEQ
                     NNVIGNVGQGGQAFVRGIGQEGNIRITWIEEGKSVSCFAHYQQNTTSEKIAQSIILNG
                     LRCQIQ"
     gene            4232..4804
                     /locus_tag="ACHKUC_004011"
     CDS             4232..4804
                     /locus_tag="ACHKUC_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6620179.1"
                     /translation="MIKTTPHKITILMGLLLSPSVFATDVDVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPNMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFSYQ"
     gene            4816..5424
                     /gene="yadL"
                     /locus_tag="ACHKUC_004012"
     CDS             4816..5424
                     /gene="yadL"
                     /locus_tag="ACHKUC_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO6620180.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGSAKLSAINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIELTPR
                     ALCEGNSNNGPGFANASTAAAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTDVTAGNFTAPATFTVTYN"
     gene            5451..6047
                     /locus_tag="ACHKUC_004013"
     CDS             5451..6047
                     /locus_tag="ACHKUC_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6620181.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNAGAKNVGVVIFSIQYPANTFNVLGATGRSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPTLNVTSGPLISHVLVDIYYE"
     gene            6073..>7173
                     /locus_tag="ACHKUC_004014"
     CDS             6073..>7173
                     /locus_tag="ACHKUC_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO6620182.1"
                     /translation="MQHDNGVKMKTFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSDKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINYHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLENTKSAQMVLKP"
BASE COUNT         2170 a         1560 c         1544 g         1899 t
ORIGIN      
        1 aatgtctgtc caatgatgaa tgaatgttat acttggtaaa aaacaaacag acatcattaa
       61 caaagtaacg ttcagttaca tggcattgca tcaaaatctc atcatcatgc aatgctgtta
      121 tctgctttat ggaatattga taaaggattt ttatgtctaa aaaattaggt tttgtgctgt
      181 ccggtattat gttggcaatg gctgctggta ctgcatttgc tgctaacgat atggacggtg
      241 gtcagttaaa tattagcggc ctggtcgttg ataacacctg tgaagctcgt gttgatggcg
      301 gtaacaaaga cggtctgatc ctgctgcaaa ctgctaccgt tgctgaaatc accgatggtg
      361 tactggatac caccgtaggt gctaaagcta aaccattcag cattactatt gactgtagca
      421 aagctaaccc ggctccgggt actactgcaa aaatgacctt cggttctgtt ttctttggta
      481 acagcaaagg aacactgaac aacgacatgt ctattaacac cccgtctgac ggcgtaaata
      541 tcgctctgca caatattgat ggttctgcta ttaaacaggt tcaggtcaat aatccgggtg
      601 atgtttacag caaaactctg gattcaacgt ctaaatctgc aacctacgat tttaaagcat
      661 cctatgttcg tgcggatgca agcaaagcag ctactgccgg ttatgtaaaa accaactcag
      721 catataccat tacttaccag taaatatcaa ccgcttaaat tgatcggatg aatatataca
      781 gggaataaca tcaaatattt ttacttttga taattattcc ctgttttaat taattctgta
      841 aggaatggtt tatgtttttt aatactaaac ataccgcagc attgtgctta gcgagctgta
      901 tggcttttag ctcatcagct attgcagaca tcgtaatttc aggtacacgc gtagtttata
      961 aaagcgaaca aaaaaacgtc aatgtacgtc tggaaaataa aggcaataac ccattgctgg
     1021 ttcagagttg gttagatact ggcgatgaca acgctgaacc aggcagtatt actgtcccct
     1081 ttaccgctac gccgccagta tcacgtattg atgctaaacg tggccaaaca atcaaactga
     1141 tgtacaccgc cagcagtgct ctgccaaaag acagagaaag tgtgttctgg tttaacgtac
     1201 tggaagtacc tccaaaacca gatgcagcaa aagcagcaaa ccaaagcctg ctacaactgg
     1261 catttcgcac acgtataaaa ctgttctatc gcccggaagg attgagtgga atttcatctg
     1321 atgcaccatt ggctctgaag tggtcctggg caacatctga aggcaaagca gcattacgcg
     1381 ttgataaccc tacgccctac tatgtctctt ttagcagtgg tgatttagaa gccaacggca
     1441 aacgctaccc cctggatatg acaatgattg cgccgttcag taacgacgtc atcaaggtaa
     1501 atgggatgag tggcagaaca agctctgcaa aagtgcattt tttcgccatt aatgattttg
     1561 gtggatctat tgaaggcaac gccaaccttt aatctggcaa ggatataaca cagcaggaag
     1621 cagaacgtga ctattaaatc tactaatcac cttacccaca tcgccacgtt ttgcgctctg
     1681 ctgtatagca atagtgcttt atgcgctgaa ctcgtcgaat atgaccatac atttctgatg
     1741 ggaaaggatg cttcaaatat cgaccttagt cggtatacag aaggcaatcc cacactacct
     1801 ggcatttacg atgtcagtgt gtatgttaac gatcaaccca tcatgagcca aagcattgct
     1861 tttgctgtta ttgaaggtaa aaagaacgct caggcctgca ttacgcagaa aaatttattg
     1921 caattccata tcagttcacc agacaagaat agcgaaaaag ccattttgct taagcgagac
     1981 gaagatcttg gtgattgcct caatctggct gaaatgatcc ctcaatcatc cattcgttac
     2041 gatgttaacg atcaacgcct ggatatcgat gtcccccagg catggattat gaagaattat
     2101 caaaactatg ttgatccctc tttgtgggaa aatggcatta atgcagccat gctttcctac
     2161 aacctcaatg gatatcacag tgaaagtcca ggcagaacaa atgacagtat ctatgcggca
     2221 ttcaacggtg ggataaactt aggcgcgtgg cggttacgtg cctcaggtaa ctacaactgg
     2281 atgaccaatg tccatagcga ttacgatttt cagaatcgct atttacaacg cgatcttgcg
     2341 tcactacgtt cacaactggt tattggtgaa tcttacacga ccggcgagac cttcgattca
     2401 gtcagcattc gcggtattcg cttatacagt gacagtcgaa tgctgcctcc ggtattagcc
     2461 agttttgcgc caatcattca tggcgtagcg aataccaatg ccaaagtaac cgttatgcaa
     2521 aatggttata aaatctatga aacgacggtg ccgccagggg cctttgccat tgacgatctt
     2581 agcccgtcag gttatggcag cgatctcatt gtgactatcg aagaagctga tggcacaaaa
     2641 cggacgttct ctcagccatt ttcatctgtt gtgcaaatgc tgcgccctgg cgttggtcgc
     2701 tgggatatta gcgccggtca ggttttaaaa gacagtatcc aggatgagcc taatttattt
     2761 caagccagct actactatgg tttgaataac tacctgacag gatataccgg tattcagcta
     2821 actgataata actacaccgc cggattgctg gggcttggta tgaatacccc cgtgggggct
     2881 ttttctgtcg atgttactca ctcaaatgtc agcattccgg acgataaaac ctatcaagga
     2941 caaagctacc gtatttcctg gaacaagtta tttgaaaata ccagtacatc attaaatatc
     3001 gcggcctatc gctactccac ccagcattat ctgggcctca atgatgcgct gacgctgatt
     3061 gatgaagtag aacatcctga gcaagatctt gagcctaaat ccatgcgcaa ttactcgcgc
     3121 atgaaaaatc aggtcacggt cagtatcaac caaccgctga aatttgagaa aaaggattac
     3181 ggctcatttt atctctcggg aagctggtca gattactggg cctccgggca aaacagtact
     3241 aattactcta ttggttatag caatagtgcg tcctggggca gttacagtat cagtgctcaa
     3301 cgttcgttga atgaagatgg tcaaaccgac gatagcattt atctcagctt cacaattcca
     3361 atagaaaatt tactgggtac ggaacaccgc agctccggct tccagagtat tgatacacaa
     3421 ttaaacagcg actttaaggg taataaccaa ctcaatatca gcagtagtgg ttacagcgat
     3481 accaatcgta tcagctacag cgtaaacacg ggttatatga tgaataaatc cagtgatgat
     3541 ttgagttata tcggcggtta tgccagttat gaatcgccgt ggggaacgct gtcaggttca
     3601 gcttcagcaa gcagtgataa tagtcgccaa ttctctctca atactgatgg tggatttgtg
     3661 ttacatagcg gcggactgac gttcagtaat gacagtttta gtgattccga cactctggct
     3721 gtcattcagg caccaggagc aaaaggcgct cgtattaact acggaaacag caccgtagac
     3781 cgctggggtt atggtgttac cagcgccctt tcgccatacc atgaaaaccg catcgcactg
     3841 gacattaacg atctggaaaa cgatgttgaa ttgaaaagca ccagtactgt cgccgtcccg
     3901 cgccagggag ccgttgtctt tgctgatttt gaaacagttc agggacaatc ggcaattctg
     3961 aatatcgtgc gaagcgatgg taaaaatatt ccatttgcag cggatattta tgatgaacaa
     4021 aacaatgtca tcggcaacgt aggtcagggt ggtcaagcct ttgttcgcgg tatcgggcag
     4081 gaaggaaata tacgaattac ctggattgaa gagggcaaat cggtcagttg ttttgctcat
     4141 taccaacaaa acacgacatc agaaaaaata gcacaatcta ttattctgaa cggacttcgt
     4201 tgtcagatac agtaaccaca agggacttcc aatgataaaa accacaccac ataaaataac
     4261 aatactcatg ggactattgt tatcgccttc ggtatttgca acggatgttg atgtcgattt
     4321 taccgctact gtgaaagcga caacatgtaa catcaccctg acatcgctga atggttcaaa
     4381 cgtcgttgat aatggcaatg atagttatac cctgataatc cccaatatgg gactggataa
     4441 aatagtcaat aaagcgtcac aatctgaggc caacttcaaa ttagtcgcca gtgggtgtag
     4501 cagtggaatt agctggattg ataccacaat gactggaaat cagtctggta gtacatcgct
     4561 gattattcca caatcttctg actcctcctc aacaaccagt aatattggta tgggatttaa
     4621 aagattagag acttcgggcg atacgtttct gaaacccaat agcgcggaat acatccgctg
     4681 gaaaaccaca gaaattagca ccagtggtct ggaaatgact gtcgcactgc gcgaaacaac
     4741 cccaggacag ggcatacccg ggaaatttcg cgcacaggca acgttcaatt tcagctatca
     4801 ataacaagga tgacaatgat gatattaaaa aaattatgtt atggattatc gggtagtcta
     4861 tttctggctt catcactgtt aaccatttcc tcacatgcag ccactgacag tattggcctg
     4921 actgttacta ctgacgtaga aatgggtacc tgtacatcaa cgttgactga tgacggctca
     4981 gctaaacttt ccgccatcaa tttcggagat gtatatattt ctgaaattaa tgccgaaact
     5041 aaaattaaaa ccttcaaatt acagttcaaa gactgtgcgg ggattcccgg gaaaaaggcc
     5101 aaaatagaat tgactccacg agcgttatgt gaaggaaatt ctaataatgg tcctggattt
     5161 gctaacgcat caacggcagc agcaaaagca gcggcggtcg ccgttgaggt ctggagcaca
     5221 tctacaccag gtaagaacgg cgctaaacaa ttcagttgtg tcacccccgc gacagaagaa
     5281 gtcagtattg ctaatgcgac aggaagtaat gttgtcgatt acccaatgag tgcggtgttg
     5341 gtggtcgcaa aagataaaac cgttacagat gtcaccgcgg gtaatttcac tgccccggca
     5401 acattcaccg tgacttacaa ctaacaggca gactgattca ggaactaaat atgcgtccca
     5461 ctcaacgtaa tctgatgaaa aaatcaattc tgattctgtc gttgctattc agcgccactt
     5521 cccctgtggt tgcaggacag gatgttgaca ttgttgcaaa tataaaaaat aacacctgcc
     5581 aaagtggaat cagcaacaac ggaaatattg atttaggtgt cgttggcgtg ggcgatttta
     5641 ccggaaacat ttcagccgaa aattatcacc ccggtggaaa agagttcact attacagtac
     5701 aggactgcac acttcagggc acagggaatg tgttaaatca gttacatatt gattttcgtg
     5761 cactcagtgg cgtaatggcg acaggttcca gtcaaatatt cgctaatgaa gacaacgccg
     5821 gggctaaaaa tgttggggta gttatattct ctattcagta cccggcaaat accttcaacg
     5881 ttctcggcgc cactggtcgc tcacgttccg tttatccggt catgtcatca gcactaaaca
     5941 attcatcctg gaaatttagc acccgaatgc aaaaaatcga tccaacactg aatgtcacct
     6001 ctggcccact gataagccat gtgttagtgg atatctacta cgaataacag acaaagtgct
     6061 acaagtatta tcttgcagca tgacaatgga gtaaaaatga agacattctt cagatacttt
     6121 ctttttttag cactatgctc ttgttgttat acagcaagtg ccggcaccga tgataacgtc
     6181 agctatcttg ttgggaatga ttacggagtg ggtccgggag accaaaagtg gagagaaaca
     6241 ggccccagtg cagacattac tgtcaaattc aggtattcaa caagcacaaa taacttagtt
     6301 ttttataaac caacccaact cggaccaaca ggtgtgtcat tatactggtc tcagttaaat
     6361 agcgcttcag gtggaggtta cctttactgc aacagaagta atactgcaag cggtgagcct
     6421 atggcaattg aaaatcaaat ggttgattcg ggtaaacgtt atggcgggca taaactattt
     6481 gaaacatctg tccctggcct gtattataca ttaaagatat caaacatttg gtcatcatac
     6541 accacaacca atatcagttc atcaggtatg tatatcggcg attctccaac tcaatatttt
     6601 cggtggatcc ccgaaagtga agccactcta tattggtctt gtaacaatgc aaactcatct
     6661 gataaatact gggccgtagg tggagtttgg cagtcgctca cggtggaatt ttataccgat
     6721 accaactttg accccacaaa gacaaacaag gtaacattat ctaagccggg tggttatctc
     6781 tactcattca aaggatatgg tcttggtggc ggtatcaact atcacagcta tttccttaaa
     6841 atagattttg acctggataa tatgacgtta tccaacccaa cgtgttttac tgctgcactt
     6901 actggcccat ctgtaagcgg ttcaacagtt aaaatgggag attatacccc agcgcaaata
     6961 aaaaatggcg caacagcggt tccttttgat atcacgctac aaaactgtat tcgtgttcgc
     7021 aatatagaaa caaaacttat gtcaaacaag gtgggcagtg taagcaaaga acttctggct
     7081 aacacactca caggaaataa cgcagcaaaa ggcgttggag tactgattga aggtttagaa
     7141 aacactaaaa gtgcacaaat ggtactgaaa cca
//
LOCUS       DAZFRG010000230         7148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFRG010000230 DAZFRG010000000
VERSION     DAZFRG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.231"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..168
                     /locus_tag="ACHKUC_004015"
     CDS             <1..168
                     /locus_tag="ACHKUC_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFO6620183.1"
                     /translation="YRGSPVYFSQWKICNSFLALASPLITPKITIELFPMQSQLYHHD
                     YQLIHSSNGQN"
     gene            314..1164
                     /locus_tag="ACHKUC_004016"
                     /pseudo
     CDS             314..1164
                     /locus_tag="ACHKUC_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1113..1382
                     /locus_tag="ACHKUC_004017"
     CDS             1113..1382
                     /locus_tag="ACHKUC_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6620184.1"
                     /translation="MPTAPLFGRDLKKCMELKWVYLHCCYDNACVESFFHSLKVECLH
                     GEHFISREIMRATVFNYIECDYSRWRRHSWCGGLTLEQFENQNLA"
     gene            complement(2189..3124)
                     /gene="ldtA"
                     /locus_tag="ACHKUC_004018"
     CDS             complement(2189..3124)
                     /gene="ldtA"
                     /locus_tag="ACHKUC_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6620185.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(3186..4265)
                     /gene="cobT"
                     /locus_tag="ACHKUC_004019"
     CDS             complement(3186..4265)
                     /gene="cobT"
                     /locus_tag="ACHKUC_004019"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO6620186.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(4277..5020)
                     /gene="cobS"
                     /locus_tag="ACHKUC_004020"
     CDS             complement(4277..5020)
                     /gene="cobS"
                     /locus_tag="ACHKUC_004020"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO6620187.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            complement(5017..5562)
                     /gene="cobU"
                     /locus_tag="ACHKUC_004021"
     CDS             complement(5017..5562)
                     /gene="cobU"
                     /locus_tag="ACHKUC_004021"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO6620188.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(5714..5887)
                     /locus_tag="ACHKUC_004022"
                     /pseudo
     CDS             complement(5714..5887)
                     /locus_tag="ACHKUC_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(5866..6054)
                     /locus_tag="ACHKUC_004023"
                     /pseudo
     CDS             complement(5866..6054)
                     /locus_tag="ACHKUC_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         1965 a         1807 c         1521 g         1855 t
ORIGIN      
        1 tatagaggtt cacctgttta tttctctcaa tggaaaatat gtaactcatt tctcgcatta
       61 gcctctccac tcatcacccc taaaataact attgagctat ttcctatgca gtcccaactt
      121 tatcaccacg actatcagct aattcattcg tcaaatgggc agaattgaat cgccacggat
      181 aatctagaca cttctgagcc gtcgataata ttgattttca tattccgtcg gtggtgtaag
      241 tatcccgcat aatcgtgcca ttcacattta gagatcatcc ggcataatca atctgccaac
      301 gaaggagagc gctatgcgta aagcccgttt tactgagcat cagatcatcg ccgtgattaa
      361 gtcggttgaa gccggacgaa ctgttaaaga tgtctgtcgc gaggccggaa tctctgaagc
      421 cacctattac aactggaagt ccaaatacgg cggcatggag gcttctgata ttaagaaaat
      481 caaggatctt gaggacgaga accgccgtct aaaacaaatg tttgccgacc tgagtctgga
      541 gaaccgggcg ctgaaagacg ttatcgaaaa aaagctttaa aaccagcctt taagcgtgag
      601 ctggttactc atctgataac gacgttcgaa ctcagtatcc gtcaggcctg ccggagtctg
      661 aacctgagct ggtctgtcga ttttatgcat gatgccctgg tctgtggccg tcgttttcgc
      721 atgttcaatg tcgttgatga ttttaaccgt gaggcattgt cgattgaaat cgatctgaat
      781 ctgccagctc agcgagtggt ccgtgtactc gacaggatcg cggcaaaccg tggctatccg
      841 gccatgcttc gtctggataa cggcccggaa tttatctccc tggcactggc tgaatgggca
      901 gagaaacatg caataaaact ggagtttatc cagccgggta agccgacgca gaacgctttc
      961 attgagcgct ttaaccggac ataccgaaca gaaatactcg atttttatct gttcagaacg
     1021 ctgaatgaag tacgggaaat cactgaaaaa tggttatcaa aatataactg tgaacgcccg
     1081 catgaatcgc tgaataatat gacaccggag gaataccgac agcgccatta tttggccggg
     1141 atctcaaaaa gtgtatggaa ctaaaatggg tctatttaca ctgctgctac gataatgcct
     1201 gcgtggaaag cttctttcat tcactgaaag tggaatgtct ccatggagaa cactttatca
     1261 gccgggaaat aatgcgggca acggtgttta attatatcga atgtgattac agtcgctggc
     1321 gacggcacag ttggtgtggc ggtctcactc tggaacaatt tgaaaaccag aacctcgctt
     1381 agagcagtgt ccatattacg agggtaggat cagtcgtttc tttcgcggta actttatatg
     1441 atcgctgtgt accagcatcg agagaagaaa ttcaggatgt attcaacact gagttcacaa
     1501 gctctgtagc tattaagtca gtattattta cgtttacgat acatgttatt atcgcaggat
     1561 ctttatcttg gtagtgatac tgcgagtgga tacaccccca ctcagtatct tctataacta
     1621 tggcttttgg ttcttcaggc atcatacctt ccataacacg ttcttgatgg atacgtaact
     1681 tgtagtactt ctctggagat gaagaaattt caacgagtac agattgtaga tgtacgaagc
     1741 ctagaaattt aaaacactcg gattttgtag aggattttaa tgataaagtc cagataacaa
     1801 acacgttatt ggaaccatca ccataatttt tagtgtcaat cttatctacc agctcctcgt
     1861 tgattaacat agactgattt agcaacttcc gcttcacgcc ctgagcggac tgttatcgct
     1921 ctttagacac agttccacta ggggagagga tcagcgcttc ccacgtaggg acactatagt
     1981 aactatcacc acatgcagta gggaagctca aaccaaattt atgtagccag ctcctattgg
     2041 tggtcattct ggtggtcttg acaggaagat aactctggtt tagcttactt attagccact
     2101 tactggcaaa ggcgatccca gtcagaggag ccaaattcaa aaaagcctgc tttcgagcag
     2161 gctttttact ttctaattac caacgctctt aaaacatctg tcttgaacca gaactaattt
     2221 gcacaggcat tcccgatcga tgttgcaacg cagcatttgc gcgatttaca tcaacttctt
     2281 gcccgttgat aaacgcccgc aaagatgggg ttaccggcaa tggcactttt cggtcagact
     2341 catattctgc acgattgcgc gacagcggct catgaacttc cagccacttt gaaccatccg
     2401 gttccgttgt gtatttcact ggctgatcaa taatttgtac acgagtgcct acaggaacat
     2461 tatcaaacag atatttgata tcgtcattac gcagacgaat acagccctga cttacacgga
     2521 gcccaatacc aaaattggca ttggtaccgt ggatggcata caacttgcca atataaatcg
     2581 cgtacagccc catggggtta tcgggccccg caggaacaaa tgcgggcaaa ctctcccctc
     2641 gtttcgcata ttcgcgccgg gtgttcggcg ttggcgtcca ggttggtgct tcttgtttac
     2701 gttcaacggt agtcacccag ttacgcgggg tttctcgccc agcctggccg ataccaatag
     2761 gaaagacttc cactgtatta ctgtctggcg gatagtaata aagacgcatt tcagcgacgt
     2821 taaccacaat ccctttacga acagtggcgg gcaaaatcag ttgctgcgga atggtgagtt
     2881 gtgagccagg cttcggcaaa aaaacatcag cgcccggatt cgcttccagc atattactta
     2941 acccttgccc gtattgtgcg gcaaaagtct ccagcggctg agtattgtga tcaggaacag
     3001 ttacagtaag cgactgcccc actaaacggc taccctctgg aggtaatgga taagttaccg
     3061 ccaggctatt ttggctggca aaaagcagag caaatgagca aagaatattt acacgacgca
     3121 tcatgtccct ttcctaagtc gcgaatacta tccgataagt atagctttta tcagactttt
     3181 cgtttttagc tgttcaaatc agaagtcgta ttccccggta gaacaatatt actggcagca
     3241 agttcgccca tgttgttgta tatcgcacaa gcagcttcga tgatggacat cgccagagct
     3301 gcaccactcc cctcacctaa acgcatatcc atattgagat aaggctccag ccccaaatgc
     3361 gagagcgcta tacgcgcgcc tttttctgcc gacaagtgag aaggaatgag atacggtttg
     3421 attgcaggag acatctggca ggctgcgagc gcagcagcat aagaaagaaa tccatccagc
     3481 aacacgggta aaccgcagga agcagcacct aacatcactc cagctattcc gaccaaatca
     3541 aatccaccca cttttgccag gacatcaaca ccatcctgag gatttggttg attcaacgta
     3601 atcgcccgac gcacaacatc aattttatta gccagtttat ctgtcggcag gtttgcgcca
     3661 atcccaacca cttcttcagg atcccggcca gtgattgtgc tgactattgc cgctgccggt
     3721 gtcgtgtttg ccatccccag ttcacctaca ccaaacagcg tgacaccgtt ttttgccagc
     3781 tcccgcgtat aacatatgac gtccaaaagc aacttttcag cctgacggcg actcattgcc
     3841 ggagctgaag caatattgcc gctacctcgt gcgacacgca tgttgataag cccggggata
     3901 ggctcagcag tatcaatacc aacatcaatt acgtggacgt tagcgcccgc ttgtgctgcc
     3961 agcacacaca cgccggttgt tccacgggtc atattttcag cctgtatggc tgtcacttct
     4021 tttggagaaa tagcgacccc ttcctcccag acgccgtgat cggcacacat aaccagtact
     4081 gcttttttgc ccacatgcgg tatgccattc aaccccggca ttcctgccag ttgtatggca
     4141 agcgcctcca gctttcccag gctaccaaca ggtttgagta acccgtcaac atgccgttgt
     4201 gcacgcgaca tagcggcaga attgatggct gggatcgtat tcagtaaatc ggcaagtatt
     4261 tgcatgtcac gtctctttat agcagtgcca gcaggaaaac cagttcacca aactctatgg
     4321 ctgctcccag cgtatcgccc gtttgcccgc ccagcgtacg tttgagaagc tgaccgagga
     4381 tgaaaatcgc caccatcgtc accaccatag cagccacacc atgcataccc ggcaataata
     4441 cggcagcaaa aatagcggct aagccgagtg tgacgcaggt ttgtcgccca tcaattttgc
     4501 caatgaacac attgccaagc ccttcttccc gcgcataacg atgacgatac atcaataatg
     4561 cggcagtccc gcggctgacc gcacatgctg ccgccagcga tgcaaggatc ggttcgccac
     4621 gcagagccaa ctcgcttaac accagaatct ttgccagtac cacaaaaatc aatgccagac
     4681 cgccgtgggt gcctaaacgg ctatcacgca tgatttccaa catgcgatcg cggctacgtg
     4741 cagaaaatac gccatcacag gtatcagcga ggccgtccag atggaatccc ccggtcatca
     4801 gcgccagcac aagtacgcta aacagtgccg ccagtggtac gccacaccat gcctgcagca
     4861 ccatgaagac cagcccgcta atcgcgccaa gcaataatcc aatcaaagga aaagtaataa
     4921 taccgcgaga ataatgctcg aaatccagtc cctgggacca gcgacgcggt acaggcagac
     4981 gcgtaataaa tgagagcatc gcccaaaata atttactcat ttgattttta ctccaatacc
     5041 cgaaaccacc agccatactt catttgccgc agcggccaac tgctgattta cccgcccggc
     5101 aatatcacga aaatgtcgtg ccagacgact ctccggcaca atccccattc ccacttcgtt
     5161 agtcactaat acaacctttg cggggcaacg ttggcaggca gcaatcaacg actgaatctc
     5221 agcattaatc gcctgttcca tcgcctgata atcccattca tcagggtctt tatcgccgcc
     5281 ataatcaaac aacagattag tcaccattgt ggtaacgcat tccagcaaca cagcctcatt
     5341 cgggttaatg tctgcatgaa ttaattcatc aagatgttgc cagcgctcca ctgtgcgcca
     5401 gtgctccggg cggctttgcc gatgatgttc tatccgtgca gccatctcat catcaaggat
     5461 ttgcgaggta gcgatataca gaacctgtga agagtcccca ataagcacct ctgcgtggcg
     5521 actcttcccg ctccgtgcgc ctcccgtcac caaaatcatc ataccgactc ctgattcaaa
     5581 tgtatgatgg tattcattga acagagcaat ggatccccca ccgttgctga ccgcaaaaca
     5641 tttacgcgat taatactgcg cgtaatataa ttaaaaatcc agcattctca atccattacg
     5701 aaataagatg gcattacgga atagccagac tttctcgtgt taacgtccga caaaatgcgt
     5761 cattgacagc ggtttctgtg gattgacaca tttcattcag ataatgaatt aatgctactg
     5821 ccggaccaac cagcaaacgg gcattgtgtt ctgaaatcca ggctattgat tcaacgtcag
     5881 cgacagatct gcgctgtaaa agaagttgta aaacaacgca ataaaattat ccggaatggt
     5941 ggcgactatg cactagaaca ccacaatttc gctctctcgg taaggcactg tcacagcatt
     6001 caacagaatg tgacttgcca gatttattag cgccaccaga tgtttaaccg ggtaaccacg
     6061 acccagtcgg ggatatattc catcttctcg ctgacatcat caagaataaa aaggtttatc
     6121 acactcagag caacgttctt cagcaggcgg gataattctt caacatttaa aagatgtgtt
     6181 attaaaggca tacaaactgg acgtattgta ttttcttttg gtggttgacc taaggtagca
     6241 gtttatcctg atgcgctgag atttctgtaa tatctacgtc aacattctcc tcgaatagaa
     6301 aatgcagcct cggcaagtga ttcacatttt ttaacaaagc gttgttagcg tgccattttc
     6361 agaagctctc caagaaagcg aattcttttc tcaaattctg cattctcaga cacagctttc
     6421 tactccatgg caatggccca aatacgaagt tgctcaggat cattagtggt gctgagaagt
     6481 taaataacca tgcggagtac cactcgtcat cacgtattta tatatcatga agctattgtt
     6541 gtgttttgta catcagtaga tgtattctga tatactcctt tttctagaca taacctttca
     6601 cctgcttgca aagcttctgt gttctgacat tgccaaattg ttgcaattct gtatccagcc
     6661 ttctttcagt catagcttcg ggccgcgata agactcactg atctgaccct gattcctctt
     6721 gcagacttta tagaccaatt aaaatgcagt ttctgcaggt caacgtctga ccatcattgt
     6781 catcactctg accattagag taaccttctg cattcatcct tttgtaaaaa gtttatgtca
     6841 gtatcagcaa ttaaccggac ctgatactga tatgagtctt accgcatata cggtcaattt
     6901 cagcaattaa ttacattatc cacgccaaag tgtttgtcat cgcaatgatg gtaccttctt
     6961 tcagacacca ttttttcaac tccgttttcc acggaccgca ctcttatgtc aagagtgcgg
     7021 tccgtggata caaccagaga ccgactgaca cgagtcagag gaaacgacgg atatgttcag
     7081 tcgtaaaata tctatcaaaa aacatgatta aggtcaaaaa tgtttgatat ttacaattta
     7141 tgaagatg
//
LOCUS       DAZFRG010000231         7143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFRG010000231 DAZFRG010000000
VERSION     DAZFRG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.232"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            162..380
                     /locus_tag="ACHKUC_004024"
     CDS             162..380
                     /locus_tag="ACHKUC_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6620189.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(596..1525)
                     /gene="fdhE"
                     /locus_tag="ACHKUC_004025"
     CDS             complement(596..1525)
                     /gene="fdhE"
                     /locus_tag="ACHKUC_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO6620190.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1522..2157)
                     /gene="fdoI"
                     /locus_tag="ACHKUC_004026"
     CDS             complement(1522..2157)
                     /gene="fdoI"
                     /locus_tag="ACHKUC_004026"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6620191.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2154..3056)
                     /gene="fdoH"
                     /locus_tag="ACHKUC_004027"
     CDS             complement(2154..3056)
                     /gene="fdoH"
                     /locus_tag="ACHKUC_004027"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO6620192.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3069..6119)
                     /gene="fdnG"
                     /locus_tag="ACHKUC_004028"
     CDS             complement(3069..6119)
                     /gene="fdnG"
                     /locus_tag="ACHKUC_004028"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5532..5534),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6620193.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6313..>7143
                     /gene="fdhD"
                     /locus_tag="ACHKUC_004029"
     CDS             6313..>7143
                     /gene="fdhD"
                     /locus_tag="ACHKUC_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO6620194.1"
                     /translation="MKKTQQKEIENVTSVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1595 a         1900 c         1912 g         1736 t
ORIGIN      
        1 accatttttg agtaacaatt gaaataactg aaactgttac attatctttg gatgccgtct
       61 cgcaaaaaca aaacaatatg aaaaaatccg ctttcgttaa ttaactttcc ggcgtaattt
      121 tcgcacatac attaatgagt aatatatgta aggtggtggt aatggcgatg aatacggttt
      181 ttcttcattt atcagaagag gcaattaaac ggctgaacaa gcttcgggga tggcgtaagg
      241 tttcgcgctc tgcaatttta cgcgaagcgg tagagcaata tctggagcgg cagcaatttc
      301 cggtgcgtaa ggcaaaaggc ggcaggcaaa gggacgaggc agttggtgtt gaagaactat
      361 gtaagcagca taaggaatga caaaagtttt tttattcatg aatataaaaa accagatgcc
      421 cttattctgg tattaataca aggctgtttt acttgaactt ataataactg caactgttac
      481 atcatatctg gaaaacgcct cgcgaaatac gagagatttt gcgggaatag caggtgtcgt
      541 cacaccctat gagctgtaat ccgggcgacc agcgccaccc ggaaaacgtc agagattact
      601 ccccttcacc cggaaacagg aacgggttaa tggaactacg ggcatagcct tcttgctcca
      661 ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac ggcttcaact ttcggttctt
      721 tttcctgata aagaatcttc aggtaagtgc cgcagtcatc gcagctttcg gctttaatcg
      781 cggcctgttc gtcatccagc gaccagtaat gcagtttgcc gctttgttca cagttgctgc
      841 attttacgcg cactacgtgc cattcagttt cacacaggtt gcagtgcagg taacgcagac
      901 cctgagtggt gccaatttgc accatgctgg acaccggcat agagccacat accgggcaat
      961 attgacgttg ttcgccgtat tcagcgcggg ctttgccggg gatcagatta gccatctgcg
     1021 cccagtagag cgacagcgca gcccagataa acggcgcttt atcgctgctg acggacgaga
     1081 aatcagaggc aaacagtgcg ctggccatat cttccagctc ctgagtcgat gccttctcca
     1141 gattctcaat cactgccagc gccgggccgc tcatttcagg tttcagctca gcaatcagcg
     1201 ccatcagcag cttttgccag tgcttatcac gcggcagaac gtgaatatcc agcgggggct
     1261 tgccttgtgc gctggcttct ttaatgcgcg tggtcagatc catctccagc ggatggtcgt
     1321 acagcaccac ttcctgggcg tgggcgataa gcgcagcaaa gcgcaggtaa tcacccagcg
     1381 gattattttc tgccagctcg cgcagacgct cggcgcggcg gttgtataaa ttcttgagcc
     1441 gagggaacaa taacggcgga atcatatccg ccgtacgttt ctcgctcgaa cccagctcat
     1501 cttgcgggat tatgcgaata ctcattcagc tttcttttcc gttgtcttgc ggacctcacg
     1561 gtaccagcgc gggtgatgtt tctttgccca tgcgctggtc acccatcctt ccaccatggc
     1621 ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc accatgataa ccacaattaa
     1681 cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg atcaccggga ttgagaaagc
     1741 aggcgcaaaa taaggacgcc agataatcac gccgctcacc agcaacagga ccaggaaaat
     1801 aatcgccgcc cagaaaacgc atttctgacc gaagttataa cgcccggtgt cacctacttc
     1861 ctcgttgacg acgatcttac gaatattctt cgcccaaaag atatcatccc gattgattag
     1921 gttgtgatgc cagtaacgga aaaacatgat gatgaacgag gcaaacataa ccacgccgac
     1981 aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg atttgcatca accagttgaa
     2041 ggacgggaac aaaaagccca gcccgctcac cgccgccagg atgaagcaga aggcggtgat
     2101 ccagtggttg atacgttccg gcgcggtgta gcgcacgatg gtgtcacgtc gtttcatttg
     2161 cgctcctcgt ctttctcttc gtgcagatta ttctcttcct catccgcacg gttcggaccg
     2221 acacccacgt agtggaagat actggctgcg aaggtagccg caaagccaac agctgcgagc
     2281 ggtttccaga tgcctttcca gaatttcacg gtttcgctga tttccgggtt ctccggcaag
     2341 ccatgataca gatttggctt gtcagcatgg tgcagcacgt acataacatg tgtaccaccg
     2401 acgcctgccg ggtcgtacaa acccgcattg tcgtaaccac gggttttcag ctccgccacg
     2461 cgctcgctcg ccagcgtttt catcgactct ttcgtaccga agtgaatcgc gcccgttggg
     2521 caggtcttca cgcaggccgg ttcttgccca accaccacgc ggtcaacgca cagcgtacat
     2581 ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt cgaacggaca gcccgcaatg
     2641 caataaccgc agccgatgca ctgctcggac tggaagtcga cgataccgtt ggcatactga
     2701 ataattgccc cttccgccgg gcacgctttc aggcagcctg gatcggaaca gtgcatacag
     2761 ccgtctttgc ggatcagcca ttccagtttg tcgttctgct ccacttccga gaatcgcatc
     2821 accgtccacg atttggcact taaatcattg gggttgtcgt acaccccaat gttattgccg
     2881 acggtatcgc gaatgtcgtt ccactctgaa cacgccacct gacaggcttt acagccgata
     2941 caggtggtga cgtcgatgag tttcgccact tcttcctgga agtcccgcgc ctgcggcgcg
     3001 ggggtcagac cgttagtcgc ggaacgacga atgatatctt gcgattgata agccatatgt
     3061 cgtctccgtt acaccttttc cacattcaca aggaaggact taaactccgg cgtctgcgtg
     3121 ttcgcatcac cgacgaatgg cgtcaacgta ttggcaataa agcctttttt cgcaacacct
     3181 tcatagcccc agtgaatcgg aataccgatg gtatcgatat ctttgccgtt tgctttcagc
     3241 gtgcgaatac gtttggtcac taccgctttg gctttgatat agccacggtt ggaggagact
     3301 ttcacagtat cgccctgggc aatgccaagt ttattcgcca gcgactcccc gatttccaca
     3361 aactgctctg gttgcaaaat cgcgttcaac agcgcgtgtt tggtccagta gtggaagtgc
     3421 tcggtcagac gataggtggt tccgacatac gggaacttat cggctttacc taatgcttcg
     3481 gcatcgtctt taaagatacg cgccgccgga ttcgagataa cgtttggatg cagcgggtta
     3541 gttcccagcg gcgtttcaaa cggctcgtag tgttccggga acggaccttc cgccatctta
     3601 tcgagggcaa acagacgtcc catgccttcc tgctgcatga taaacggccc tacgccgctg
     3661 cccggaggcg ctgcgctgta atccggaata tcccagccgg tccacttagt gccgtcccat
     3721 ttcagcaact gacgcttcgg atcccacggg ttaccctgcg gatctgcgga ggcgcggtta
     3781 tacagaatgc ggcggttaag cggccatgcc catgcccagc ccagcgtgtt accgaggcca
     3841 gacggatcgg cgttatcacg acgcgccatc tggttgcctt ccggcgtcca gctaccggcg
     3901 aaaatccagc agccacagga ggttgtgccg tcatcgcgca gttgggcgaa cgagctaagt
     3961 tgttggcctt tcttgacgat aaccgcaccg gttgccgggt cggtaatatc ggccagcgct
     4021 ttgccgttgc tctccattgc cacttcttct gaagatggct catgcggaat ggcgtagttc
     4081 caggtcatgt tcagcacctg gtccgggttc gcgccaccct gttcggcata catcttgcgc
     4141 aagcgcagga agataccgga gaggatctcg ccatcagtca gcgcaatccc cggggcgtcc
     4201 gcacctttcc agtgccactg taaccagcga cctgagttaa cgatagaacc gttctcttcc
     4261 gcgaagcagg tcgatggtag acggaacact tcggtctgga tcttcgacga gtcaacttcg
     4321 ttcagctcac cgtggttctg ccagaagtta gaggtttcag tgttcagcgg gtcgatagtc
     4381 acgaggaatt tcagcttcga cagacagccg atcactttgt ttttgttcgg gaatgaggca
     4441 acagggttaa agccctggca gatatagcca ttgaccttgc cctctttcat catctcgaag
     4501 tattgcagga cgtcgtagcc tttatcccac ttcggcaacc agtcaaagcc ccagctattt
     4561 tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag agacgaagaa tttcgggtag
     4621 ttgccccagt agttgacctg gccttccagc agtggttttg gcgtgttggc agcgaggtaa
     4681 gtttgcagat cggtctgctt ctcgcttggc aacgtcatgt aacctggcag actctgcgac
     4741 agcagcccca ggtcggtcag cccctgaata ttggagtgac cgcgcagggc gttaacgccg
     4801 ccgcctgcca tccccatgtt gccgagcagc agctggatca tcgccatcgt acgaatgttt
     4861 tgcgcaccaa tggagtgttg cgtccagccg agcgcgtaaa ggaacgaggc agttttatcg
     4921 tgagcactgg tctctgcgat gtattcgcag actttcagga acgcgtcttt cggcgtacca
     4981 cagatgtttt caaccacatc tggcgtataa cgggaaacgt gctgtttcag caagttccac
     5041 acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga agccgttttc gtccagctcg
     5101 taggtccagc tggatttatc gtacttgcgt ttttccgcgt cgtagccggt gaacaggcca
     5161 tcttcaaagc cgtaatcctc acgcacgatc aggctagcgt tggtataggc ttcggtgtat
     5221 tcgcggttga atttttcatt gttcagcagg tacagcaata cgcctgacag gaaagcaatg
     5281 tcagtcccgg aacgaatagg ggcatagtag tcagccaccg ccgccgtacg cgtaaagcga
     5341 ggatcgatca caatcagctt cgcgccgttg tgaattttgg cttccatcgc ccagcggaac
     5401 ccgaccgggt gagcttcagc ggcattaccg cccatcacca cgacgaggtt ggcattcttg
     5461 atgtcgaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc
     5521 gttggtccgt gtcagacacg cgcctggttg tcgaccgcga gcatacccag cgcgcgggag
     5581 aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg aagcacacag catcccggtg
     5641 gagagccagc ggttaacagt cacgccttcg gcgttttgcg caatgtagtt cgcatcgcgg
     5701 tcttctttca tcagtttggc gatgcgatca aacgcctctt cccaactgat ttgttgccat
     5761 ttatcagaac ctggcgcacg gtattccgga aacttcaggc ggctttcgga gtggatgaaa
     5821 tccaccaggc cagcgccttt cggacaaagt gctccgcggt tgaccgggtg atccgggtca
     5881 ccttcgatat ggaagataga tgctttggcg ttttttgctc cgtcaccgag gctgtacatc
     5941 aacagcccac agcctacgga acaataggtg caggtattac gggtttcgcg ggtgcgcagc
     6001 agtttatact gccgggtttc cgcgagtgct acgctgggtg caaaacccag tgccgctgcc
     6061 gtggtgcctg ccataccgcc agcgcagatc ttaaagaact gccttctgct gacctgcatg
     6121 gattgctcct tgtttcgaca ttgtcacgtc ccatttacat tcgcttgctg cgtgtgcagg
     6181 gagtgggagt tatttttctt tgcggaaggg gccgcaaagg tccagaattg gctcaatttc
     6241 cctccatcca ggaagggttt gtaacagaat accataatgt tggtgtgtgt gttcttatct
     6301 ggttaagaga aagtgaaaaa aacacagcaa aaagaaatcg aaaatgtgac aagtgtcaca
     6361 ggtgttcgtc aaattgagtt atggcggcgt gatgatttac aacacccacg gttagatgag
     6421 gtggcggaag aggttcccgt tgcgctggtc tacaacggca tttcgcatgt agtgatgatg
     6481 gcgtcaccca aagaccttga gtactttgcg ctcggttttt cgctttccga ggggattatc
     6541 gaaagtccgc gcgatatctt cggtatggat gtcgttcctt cctgtaatgg tctggaagtg
     6601 caaattgagc tttccagccg ccgctttatg gggttgaagg agcgtcgccg ggcactggcg
     6661 ggacgtacgg gatgtggcgt gtgcggcgtg gagcaactta atgacatcgg aaaaccggtg
     6721 cagccgctac cgttcaccca gacgtttgat ctcaacaaac tggatgatgc attacgtcat
     6781 ctcaatgatt ttcagccagt ggggcaactg accggttgta ctcacgccgc cgcctggatg
     6841 ttgccatctg gcgaactggt cggcgggcat gaagacgtgg gtcgccatgt ggcgctggat
     6901 aaactgttag gccgccggtc acaagaaggg gaaagctggc agcaaggtgc ggtgctggtt
     6961 tccagccgtg ccagttatga aatggtgcaa aagtcggcga tgtgcggcgt agagattttg
     7021 tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg ctgagcgctg taatctgaca
     7081 ctggtaggtt tttgtaaacc gggtcgggca acggtttata cccatccgca gcgtttgagc
     7141 aat
//
LOCUS       DAZFRG010000232         7116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFRG010000232 DAZFRG010000000
VERSION     DAZFRG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.233"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..199
                     /gene="ydgT"
                     /locus_tag="ACHKUC_004030"
     CDS             <1..199
                     /gene="ydgT"
                     /locus_tag="ACHKUC_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO6620195.1"
                     /translation="LLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAEL
                     VSGGRLFDLGQVPKSVWRYVQ"
     gene            285..725
                     /locus_tag="ACHKUC_004031"
     CDS             285..725
                     /locus_tag="ACHKUC_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6620196.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            802..1383
                     /gene="rsxA"
                     /locus_tag="ACHKUC_004032"
     CDS             802..1383
                     /gene="rsxA"
                     /locus_tag="ACHKUC_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO6620197.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            1383..1961
                     /gene="rsxB"
                     /locus_tag="ACHKUC_004033"
     CDS             1383..1961
                     /gene="rsxB"
                     /locus_tag="ACHKUC_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO6620198.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            1954..4083
                     /gene="rsxC"
                     /locus_tag="ACHKUC_004034"
     CDS             1954..4083
                     /gene="rsxC"
                     /locus_tag="ACHKUC_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO6620199.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDNVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVE
                     AAIARAKARKLEQQQQANAEPEEQVDPRKAAIEAAIARAKARKLEQQQANAEPEEQID
                     PRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            4084..5142
                     /gene="rsxD"
                     /locus_tag="ACHKUC_004035"
     CDS             4084..5142
                     /gene="rsxD"
                     /locus_tag="ACHKUC_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO6620200.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDTIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLATLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGMAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            5146..5766
                     /gene="rsxG"
                     /locus_tag="ACHKUC_004036"
     CDS             5146..5766
                     /gene="rsxG"
                     /locus_tag="ACHKUC_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO6620201.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            5770..6465
                     /gene="rsxE"
                     /locus_tag="ACHKUC_004037"
     CDS             5770..6465
                     /gene="rsxE"
                     /locus_tag="ACHKUC_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO6620202.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            6465..7100
                     /gene="nth"
                     /locus_tag="ACHKUC_004038"
     CDS             6465..7100
                     /gene="nth"
                     /locus_tag="ACHKUC_004038"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO6620203.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         1630 a         1831 c         1907 g         1748 t
ORIGIN      
        1 cttattaaaa tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct acgaccatct
       61 taattacacc ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg
      121 tcgcgcagag ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt
      181 ctggcgctat gtccagtaaa gaaagtagcg atcggttata aaagccttat aataacgccc
      241 ctaaaattag atttgtctgt ttgcgtcgct attctggaga acctatgacc accacaacgc
      301 cacaacgtat tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttga
      361 gtacgacgct ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa
      421 cgctcggcgg acaagcactg acgacacaaa tcttatgggg cgtttctttt attaccgcta
      481 tcgccatgtg gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg
      541 ttcctaaaca ctatattatc tggctgctta tttccgtatt gctggcagta aaagcgttcg
      601 ctttttcacc cgtagaagat ggcatagccg ttcgccagtt gctgtttact ttgttggcaa
      661 ccgcattgat cgtcccctat ttcaaacgct cgtcgcgggt gaaagcgacg tttgtgaatc
      721 cgtaataacc ttacagttaa cctgttgtcg cctgctctgg attaacggat aataggcggc
      781 ttttttattt caggccgaaa aatgactgac tacctgttac tgtttgtcgg aactgtactg
      841 gtcaataact ttgtactggt caagtttctc ggtctctgtc cgtttatggg ggtttccaaa
      901 aagctggaaa ccgcgatggg catggggctg gcaacaacgt ttgtaatgac gctggcgtct
      961 atttgcgcct ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc
     1021 accctggcgt ttattctggt gattgctgtg gttgtgcagt tcaccgaaat ggtggtgcgc
     1081 aaaaccagcc cggtacttta ccgtctgctg gggatttttt tgccgcttat caccaccaac
     1141 tgtgccgtgc tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtca
     1201 gcgctgtacg gtttttccgc cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc
     1261 atccgcgaac gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg
     1321 ttaattaccg caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg
     1381 taatgaatgc tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg
     1441 ccattctggg ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa
     1501 ttgacgaaat cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct
     1561 acgcggaagc catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag
     1621 ctgtgatgct aaaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag
     1681 cgcaagagct aacgccagcg cggatggtgg cggtgattga tgaaaataac tgtattggct
     1741 gcactaaatg tattcaggcg tgtccggtag acgccatcgt tggcgctacc cgcgccatgc
     1801 atacggtaat gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacac
     1861 actgcatctc gttgcaaccg gtcgcagaaa ctcctgactc ctggaaatgg gatctgaaca
     1921 ccattcccgt gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga
     1981 aaaaataaaa tctgggattt caacggcggc atccatccac cggagatgaa aacccagtcc
     2041 aacggtacgc ccctgcgcca ggtaccactg gcgcagcgtt ttgtcattcc actgaaacaa
     2101 catattggcg ctgaaggtga gttgtgcgtt agcgtcggcg ataacgtatt gcgcggtcag
     2161 ccgcttaccc gtggtcgcgg caaaatgctg ccggttcacg cgcccacctc gggtaccgtt
     2221 acggctattg cgccccactc tacggctcat ccttcagctt tagctgaatt aagtgtgatt
     2281 attgatgccg atggcgaaga ctgctggatc ccgcgcgacg gctgggccga ttatcgctct
     2341 cgcagtcgcg aagagttaat cgagcgcatt catcagtttg gtgttgccgg gctgggtggt
     2401 gcgggatttc cgacaggcgt taaattgcag ggcggcggag ataagattga aacgttgatt
     2461 atcaacgctg ctgagtgcga gccgtacatt accgccgatg accgtttgat gcaggattgt
     2521 gcggctcagg tcgtagaggg tatccgcatc cttgcgcata ttctgcagcc acgcgaaatt
     2581 cttatcggta ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggca
     2641 gactctcacg atatttctct gcgggtgatt cccaccaaat atccttctgg cggtgctaaa
     2701 caattaacct acattctgac cgggaagcag gttccacatg gcgggcgttc atccgatatc
     2761 ggcgtattaa tgcaaaacgt cggcaccgct tatgcagtga aacgtgccgt tattgatggc
     2821 gagccgatta ccgagcgtgt cgtgaccctg actggcgaag caatcgctcg cccgggcaac
     2881 gtctgggcac ggttggggac gccagtgcgt catttattga atgacgccgg attctgcccc
     2941 tctgccgacc aaatggtgat tatgggcggc ccgctaatgg gctttacctt gccgtggctg
     3001 gatgtcccgg tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgaacttggc
     3061 gaaccacagg aagaacaaag ttgcatccgt tgtagcgcct gtgctgacgc ctgccctgcg
     3121 gatcttttgc cgcaacagct gtactggttc agcaaaggtc agcaacacga taaagccacc
     3181 acgcataaca ttgccgactg cattgaatgt ggagcttgcg cgtgggtttg cccgagcaat
     3241 atccccctgg tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa
     3301 gaaaagcgcg ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc
     3361 gaaaaagcgg ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat
     3421 aaagatgcga ttgctgccgc cctggcgcgg gtgaaagaga aacaggccca ggctacacag
     3481 cctattgtga ttaaagcggg cgaacgcccg gataacagtg caattattgc agcacgggaa
     3541 gcccgtaaag cgcaagccag agcgaaacag gcagaactgc agcaaactaa cgacgctgca
     3601 accgttgctg atccacgtaa aactgcagtt gaagcggcta ttgcccgcgc caaagcacgc
     3661 aagctggaac agcaacaagc caatgcggag ccagaacaac aggtcgatcc gcgcaaagcc
     3721 gccgtcgaag ccgctatcgc ccgtgccaaa gcacgcaagc tggaacagca acaggccaat
     3781 gcggagccag aagaacagat tgatccgcgc aaagctgccg tcgaagcggc tattgcccgt
     3841 gccaaagcgc gcaagctgga acagcaacaa caggctaatg cggaaccaga agaacaggtt
     3901 gatccacgca aagccgccat cgaagcggct attgcacgcg ccaaagcgcg caagctggaa
     3961 cagcaacagg ctaatgcaga gccagaagaa cagattgatc cgcgcaaagc ggcagttgct
     4021 gcggctattg cccgcgttca ggccaaaaaa gccgcccagc aaaaggttgt aaacgaggac
     4081 taaatggtat tcagaatagc tagctcccct tatacccata accagcgcca gacatcgcgc
     4141 attatgctgt tggtattgct cgcagccgtg ccaggaatcg cagcgcaact gtggtttttt
     4201 ggttggggta ctctcgttca gatcctgttg gcgtcggtca gtgctcttct ggccgaagct
     4261 ctcgtcctca aactacgcaa gcagtcggta gctgcaacgt tgaaagataa ctcagcattg
     4321 ctgacaggct tattgctggc ggtaagtatt ccccccctcg cgccatggtg gatggtcgtg
     4381 ctgggtacgg tgtttgcggt gatcatcgct aaacagttgt atggcggtct ggggcaaaac
     4441 ccgtttaatc cggcaatgat tggttatgtg gtcttactga tctccttccc ggtgcagatg
     4501 accagttggt taccgccaca tgaaattgcg gtcaacatcc ctggttttat cgacaccata
     4561 caggttattt tcagtggtca taccaccagt ggtggtgata tgaacacgct acgcttaggt
     4621 attgatggca ttagtcaggc gacaccgctg gatacattta aaacctctgt ccgtgccggt
     4681 cattcggttg aagagattat gcaatatccg atctacagcg gtattctggc gggcgctggt
     4741 tggcaatggg taaatctcgc ctggctggct ggcggcgtgt ggttgctatg gcagaaagcg
     4801 attcgctggc atattcccct cagcttctta gcaacgttgg cgttatgcgc aacgttgggc
     4861 tggttgttct caccagatac tctggcagca ccgcaaattc atctgctgtc tggtgcgacc
     4921 atgctcggcg cattctttat tttgactgac ccggttaccg cttctacgac caatcgtggt
     4981 cgtcttattt tcggcgcgct ggcgggctta ttagtctggt tgatccgcag tttcggcggc
     5041 tatcctgacg gcatggcttt tgccgtcctg ctggcgaaca tcacggttcc tctgatcgat
     5101 tactacacgc gtccgcgcgt ctacggtcat cgcaaagggt aaaccatgct gaaaactatc
     5161 cgaaaacacg gcattacgtt ggcgctcttt gcagcgggtt caacagggtt aactgcggcc
     5221 atcaaccaga tgaccaaaac gacaattgct gaacaggcca gtctgcaaca aaaggcgtta
     5281 tttgatcagg tgctgccagc tgaacgctat aacaatgcgc tggcacagag ttgctatctg
     5341 gtcactgcgc cagagttagg taaaggtgag catcgggttt acatcgccaa acaggatgac
     5401 aaaccggtag ctgccgttct ggaagcaacc gcgccagatg gctattcagg tgcgattcag
     5461 ctgctggtag gagccgattt taacggcacg gtacttggca cgcgcgtgac tgagcaccac
     5521 gaaacgccag ggcttggcga taaaatcgaa ctgcgccttt ctgactggat cacccatttt
     5581 gcgggtaaaa aaatcagtgg tgcggatgat gcgaactggg cggtgaagaa agatggtggt
     5641 gatttcgacc agttcaccgg cgcgacgatt actccccgcg cggtggttaa tgcggtaaaa
     5701 cgcgccggat tgtacgctca gacgttaccc gcacaacttt ctcaacttcc tgcctgtgga
     5761 gaataaaacg tgagcgaaat taaagacgtt attgttcagg ggttgtggaa aaacaactct
     5821 gcgctggtcc agctgctcgg cctttgtcct ctgttggcgg tcacgtccac tgccactaac
     5881 gctctgggtt taggacttgc gactacgctg gtactgacgc taaccaacct gaccatttcg
     5941 acgctgcgtc actggacacc cgccgagatc cgcattccca tttacgtgat gatcatcgcc
     6001 tcggtggtca gcgctgtaca gatgctgatc aacgcctacg cctttggcct gtatcaatca
     6061 ttagggattt ttattccgct gattgtcact aactgtatcg ttgtgggccg cgctgaagcc
     6121 ttcgccgcca aaaaaggtcc ggcgctttcg gcactggacg gcttttcaat tggtatgggc
     6181 gcaacctgcg ccatgttcgt gctgggttca ctacgcgaaa ttatcggcaa tggcacgttg
     6241 tttgacggtg cagatgcgct gttaggtagc tgggcgaaag tattacgcgt ggagattttc
     6301 cacaccgact cccctttcct gctggcgatg ctgccaccag gtgcatttat tggcctggga
     6361 ctgatgctgg caggaaaata cctgattgat gaaaaaatga aaaagcgccg tactgaagca
     6421 gctgccgaac gtgcattgcc aaacggtgaa acagggaatg tctgatgaac aaagcaaaac
     6481 gcctggagat cctcactcgc ctgcgtgaga acaatcctca tcccaccacc gagcttaatt
     6541 tcagttcgcc ttttgaattg ctgattgccg tactgctttc cgctcaggcg accgatgtca
     6601 gtgttaataa ggcgacggcg aaactctacc cggtggctaa tacgcctgca gcgatgcttg
     6661 aactgggcgt tgaaggggtg aaaacctata tcaaaacgat tggcctttat aacagcaaag
     6721 cagaaaatat catcaaaacc tgccgtatat tactggaaca acataatggc gaggttccgg
     6781 aagatcgtgc tgcgcttgaa gccctgcctg gtgtaggtcg taaaacagcc aacgtcgtat
     6841 taaacactgc attcggctgg ccgactattg ctgtcgacac gcacattttc cgcgtttgta
     6901 atcgtactca atttgcgccg gggaaaaacg tcgaacaggt agaagaaaag ctactgaaag
     6961 tggttccggc agagtttaaa gtcgactgcc accattggtt gatcctgcac gggcgttata
     7021 cctgcattgc ccgcaagcct cgctgtggct cttgtattat tgaagatctt tgtgaataca
     7081 aagagaaagt tgacatctaa agatcagggg taacac
//
LOCUS       DAZFRG010000233         7063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFRG010000233 DAZFRG010000000
VERSION     DAZFRG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.234"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..1914
                     /locus_tag="ACHKUC_004039"
     CDS             136..1914
                     /locus_tag="ACHKUC_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6620204.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1954..2433)
                     /gene="xdhC"
                     /locus_tag="ACHKUC_004040"
     CDS             complement(1954..2433)
                     /gene="xdhC"
                     /locus_tag="ACHKUC_004040"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO6620205.1"
                     /translation="MNHSETITIECVINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2430..3308)
                     /gene="xdhB"
                     /locus_tag="ACHKUC_004041"
     CDS             complement(2430..3308)
                     /gene="xdhB"
                     /locus_tag="ACHKUC_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO6620206.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGSDVLIQLHHHND
                     RYRHIVDIHNLAELQGITQAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3319..5616)
                     /gene="xdhA"
                     /locus_tag="ACHKUC_004042"
     CDS             complement(3319..5616)
                     /gene="xdhA"
                     /locus_tag="ACHKUC_004042"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO6620207.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6030..6785
                     /gene="actS"
                     /locus_tag="ACHKUC_004043"
     CDS             6030..6785
                     /gene="actS"
                     /locus_tag="ACHKUC_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO6620208.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6864..6937
                     /locus_tag="ACHKUC_004044"
     tRNA            6864..6937
                     /locus_tag="ACHKUC_004044"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6896..6898,aa:Gly,seq:ccc)
BASE COUNT         1766 a         1689 c         1792 g         1816 t
ORIGIN      
        1 cataatttta ttagaaagtg ataaaaatct tatgttttta tcaaagccaa tttcgcctga
       61 gttaatatca tcatgatctg tgaaccatca acgtcttcaa tgatcgactt ttaagtaagt
      121 ctggaaggta aagctatgga gcttgcgact acgcagtcag tattgatgca aattcaaccg
      181 acaattcagc gttttgccag aatgcttgcc agcgttttgc agcttgaggt tgagatcgtt
      241 gatgaaaact tgtgtcgcgt cgccggaacg ggcgcgtatg ggaagtttct tggccgccag
      301 ttgagcggca actcacgcct gctccgccac gtcctggaaa cgaaaactga aaaagttgtg
      361 acacagtctc gcttcgatcc cctttgtgaa ggttgcgata gtaaagaaaa ttgccgcgaa
      421 aaagcatttc tgggtacgcc tgtcatttta caggatcgtt gtgttggggt gataagtttg
      481 attgccgtta cccacgagca acaagaacat atcagtgata atttacgcga attttccgat
      541 tacgttcgcc atatatccac catttttgtt tctaaacttc tggaggatca gggaccagga
      601 gataacatca gtaaaatatt cgcgaccatg atcgataata tggatcaggg cgtgttagtg
      661 gttgatgctg ataatcgtgt tcagtttgtt aatcagacag ctttaaaaac actcggtgtt
      721 gtacaaaata atattattgg aaaacctgtt cgtttcagac cattaacatt tgagagtaat
      781 tttacccatg gtcatatgca acatattgtt tcgtgggatg ataaaagtga attaatcatt
      841 ggtcagttgc ataacatcca gggccgacaa ctatttttaa tggcgtttca ccaatcgcat
      901 accagttttt ctgtagcaaa tgcacctgat gaaccgcata ttgaacaatt ggttggcgag
      961 tgccgtgtta tgcggcaatt aaaacgactc attagccgta ttgcacccag cccatccagc
     1021 gttatggtgg ttggtgaaag cggcacgggt aaagaagtcg tcgctcgtgc aatccataag
     1081 ttgagcggaa gacggaataa accctttatt gctatcaact gtgccgcgat tccggaacag
     1141 cttctggaga gcgaactgtt cggttatgtt aaaggcgcat ttactggcgc ttctgccaac
     1201 ggtaaaacag ggttgattca ggcggcgaat acgggcacgc tgtttctcga tgaaattggt
     1261 gatatgccat taatgttgca ggctaaatta ctgcgcgcta ttgaggcgcg tgaaattctg
     1321 ccgattggtg ccagtagccc aatacaagtc gacatccgca tcatttctgc aactaatcag
     1381 aatttggccc agttcattgc cgaaggtaaa ttccgcgaag atctcttcta ccgacttaat
     1441 gttatcccga taactcttcc accgctgcgt gaacgtcagg aagatattga attactggtg
     1501 cattactttt tacatctgca tacccgtcgt ctgggatcgg tttatcctgg cattgctcct
     1561 gatgtcgtcg aaatattgcg taagcatcgt tggcccggaa acctgcgcga gttaagcaat
     1621 ttgatggaat atctggttaa cgttgttcct tcaggtgaag ttatcgacag cacgctattg
     1681 ccgccaaatc tgctgaataa tggcacaacg gagcaaagtg atgtaacaga ggtcagtgag
     1741 gcgcacctgt cactcgatga tgcgggcggc acggcgctgg aggagatgga aaagcaaatg
     1801 atccgcgagg cgctttcacg tcataacagc aagaaggaag ttgctgatga actgggcatc
     1861 ggcattgcta cgctctatcg caagattaag aaatatgagt tgttaaacac ataacctctg
     1921 agaatccggc ctgaattcag gccggatatc cttttacttc gttttctcgc aatccagaac
     1981 tgtatttaca atcatctgat accccgtgca gcgacaaaga tttcccgcca gtccacgacg
     2041 aatttccgta atggttaatg gtttttcgcg tggttttgcc agcatcgccg tggtagccat
     2101 aatcaggccc ggcgtacaaa acccgcattg cactgcgccg gatttcgcat aagccagttg
     2161 gacatgagaa agtttaccgc ctttcgcttc accttccagc gtgcggatct cttttccttc
     2221 agcccaggcc gcaaggaata agcaactgtc tatcgcagtg ccgtcgacca gcaccgtaca
     2281 ggcaccgcat tcgcctacgc agcaaccttg tttgacacta agaagccctt gttctcggag
     2341 tagttccgaa agcggcattc ctggcgcggc gtgaagctga aaaggcatcc cgttaatgac
     2401 gcattcgatg gtaattgttt cgctgtgatt cattgcaatt ttccccccgc cgcggcgacg
     2461 gcttcgctaa tcactttttt ggtcatcgtc tggatgagat gcagacgaaa ctctttactg
     2521 gcccgccatg aagaacgcgg ggcgacatct tgcaggactg attcgctgat ggcttccagc
     2581 gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg catgttggca gcgaatcggc
     2641 gttggcgcgg caacaccaaa tgccaggcgt aattcgctga aattgccgtt atccagtcgg
     2701 caatgtgcgg cgcagccaat tgttgaaata tccattgcgt cgcgcatggc atatttaaaa
     2761 tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat gaaaggcgac aaggatttcg
     2821 tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc cattaatcgg gacgaaacga
     2881 acaccgcgcg gggagtggat ctcaagcttc gcgtcataaa ttagcgttgg cgtggcagaa
     2941 tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg tagcgacgtt acggatctgc
     3001 ggtccggcaa tggatgaggc cgcagcacat aacgccggga gatgacgttg agttacagga
     3061 tcttcaatga gctgagtaaa tgttgtcgcg gagccgattc gcagcgcgcc atcttccgcc
     3121 tgtgttattc cctgaagctc tgccagattg tggatatcaa caatatggcg atagcggtca
     3181 ttgtgatggt gaagctgtat cagtacgtca gagccaccgg caagcaattt ggcctgcggg
     3241 ttgtcagcca gcaggttgat ggcatcggca agggttgctg cgcgatggta agaagcaaaa
     3301 tcaaacatga tgttatcctc aaatcaatcc tgccagatgg aattcttcat ataatcgttt
     3361 tggcgttagc ggcagtgtat tgattgcaac accggtagcc atcttcaccg cgttacgaat
     3421 agcagcggct acaggaatta tgggtggctc acccagtgac ttatgtccgt atgcggattg
     3481 cggttcattg atttcgacaa acgcgctttc cagttgtggc agatccggca tggtcggcat
     3541 tttgtaatcc agcagattgg ggttacggac cacgccgctt ttcgcatcga tgatcatctc
     3601 ttcaaatagc gcccagccaa tgcccatccc cattccgccg tgtacctgac cttctgctag
     3661 cagcggatta agaatatggc ccgaatcatg aacgttgagg atgcggttga tggtgacttt
     3721 acacagtgca atatcgaccg tcagatcaac aaaggtacag ccaaacgccg gtgggttagt
     3781 ggtggttttg atggagcttt cggcagagag ctgcccgccg cgttcagggt ggtagaaagc
     3841 gtccatcgcc aaatctttta acgacattaa cggctcttcc ggtcgttcaa ccagcacgat
     3901 atggcctttt atcagggtca gattcatcgc tgactgatgt agcatgactg cggcgtgagc
     3961 gatgattttc tcttttaata acagtgccgc actgcgcagc gcaggcgcgg caacatagct
     4021 ctgacgtgag gcaaatgcgc cggggtcgaa tggtgtaacg tcggtgtctt gtgtcgaaat
     4081 tacgcggaca tcgctgaccg gaactcccac ggtttctgcc accatttgcg agaacacggt
     4141 gtcggcaccc tggccgattt ccgtcgcgcc gctttgcacg ttgatggttc catcctgatt
     4201 catcaacagg cgcgcgcctg ctatttctac gccgacaggc caggtgttag aggtgtagct
     4261 aaaacaggcg acgccaacgc cacgacgtaa attgccttgc tggttctggc actctgcacg
     4321 gcgtttttcc cattcaaaga ttttccggcc tttttcaaga cactccggca accctgcgct
     4381 gtaaatacgt tttcccgtga gcggattagc atctccttcg cgggcggcgt tgcgtaaacg
     4441 aatttcaaca ggatcaatac ctaacgctgt cgcggcatca tcaagcatag actcaacggc
     4501 aaatacgact tgtggcgcgc cataaccacg catcgcacca gccgagggga ggttggtata
     4561 gcaggtcttt gaactgtaag cgtaggcaca acgaggataa aggtaagcga ctttattccc
     4621 cccagcagaa gcaatggagt gcccgtgaga tgcataagcg ccggtgttag acagaacatc
     4681 cagactataa cctttcaatg ttccgtcgcg gttcacgccc atttgcccgt caatagtaaa
     4741 agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg ctaagggaaa ctttcaccgg
     4801 aatgccgcca agtttgctgg tcaggaatgc cgccattggc tcttccagta catcctgttt
     4861 attaccaaaa ccgccaccga taaacggttt gatgactcgt acgcatgacc agggaatatc
     4921 cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc tgggtgctgg aaacgatggt
     4981 aattcgcgag tcatcctcca tccatgccag cgatgtcacg ctttccatat gacaatgttg
     5041 aataacggga gtctgatagt gcccctgtac ctggtagtcg gcggcatcga ttgtttgttg
     5101 gacattaccc gtcgacatcg tgctttgttt cagtaaattg ccaccgttat ggattggtgc
     5161 agcgtcttct gccagcgccg cttctggcga ggtgataacg ggtaattctt gccactcaat
     5221 gctgaccaat tgcgccgctt tttctgccgt gagttcatcg cgggccacga cgatggcaac
     5281 ggcgtcgcca tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt
     5341 gttttcgtca agtgtccagg catgccctgc cgtggcgaat gggatttctg gcacatcttc
     5401 ccaggtaaaa atcgccagga cgcccggcaa actcctggct tgttcatcat taatacttac
     5461 ggcataacca tgtgcgatag gactacgtac atatttcgcg taacacatgc ccgccataac
     5521 ataatcgtca gtatatcgtg cccgcccggt gaccttagca atggcatcga cgcgcatgca
     5581 tgattcaccc gtagcggttg cttcccgcgc ttccatagaa atcccctcgg tttgaacatt
     5641 tggttcttat ggcaaagatt tagtcttagc aggcaagaat tacgccagaa acgactatat
     5701 gcgtgagggg aggaaggaag tgtgatgacg ttaaaaaaac acagatatga tacgaaacag
     5761 gactctgaat agggacagtg atacgtatca atatgagtgg cgataaaatc aatgtgataa
     5821 attaggatta ttacgcatca aggtgggaaa tagggtaaaa gtcaggaact tcgataccac
     5881 tacagcaaat attttgctga tgatacttcc ctttgccaaa tgaaattaac ctctttctct
     5941 attacatcgc ttttccagaa gcaagagatt gggtatccag aagccggctg ctatgatatg
     6001 gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc
     6061 tgggtatcgt gatgttgtta tcggttggac tgcttttggc gggctgttcg ggtagcaaat
     6121 catccgatac aggaacgtat tccggctccg tttacaccgt gaaacggggg gatacgctat
     6181 atcgtatttc gcgcaccacg ggaaccagcg taaaagaact ggcgcgactg aacggcattt
     6241 ccccccctta caccattgaa gtaggtcaga aactaaaact gggtggggcg aaaagtagca
     6301 gtagtacacg taaatcaacc gccaaatcaa cgaccaaaac cgcatcggtt acaccgtcat
     6361 cagcggtacc gaaatcttcc tggccgccag tagggcaacg ttgttggtta tggccaacga
     6421 cagggaaagt tatcatgccg tattcgacag cagatggcgg caataaaggg attgatatct
     6481 cagctccacg gggtacacct atttacgccg cgggtgcagg aaaggtggtg tatgtgggca
     6541 accagctgcg tggctacggt aatctcatca tgattaaaca cagtgaagat tacattacgg
     6601 cttacgccca taatgacacg atgctggtaa ataatgggca aagcgtgaag gctgggcaaa
     6661 aaatcgccac tatggggagc actgatgcgg catctgttcg cctgcatttc cagattcgtt
     6721 accgtgcaac ggcaattgat ccgctacgtt acttgccgcc tcagggcagc aagccaaaat
     6781 gctgatggcg aattaatcag cagtcagcag cgtggtactt gctaaggaga gcgtaaggtt
     6841 tataatgcct tacgcatctc gaagcgggcg tagttcaatg gtagaacgag agcttcccaa
     6901 gctctatacg agggttcgat tcccttcgcc cgctccaatt tatcttcaca tcatctacct
     6961 gccatttttg ttgtatctga ttttctatat tatcttaatt caaggcttca gacttttcgc
     7021 aatcgccgta atacttcccc atttttttgt aaataaaaag cgt
//
LOCUS       DAZFRG010000234         7008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFRG010000234 DAZFRG010000000
VERSION     DAZFRG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.235"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1454
                     /gene="cscA"
                     /locus_tag="ACHKUC_004045"
     CDS             21..1454
                     /gene="cscA"
                     /locus_tag="ACHKUC_004045"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6620209.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQKNAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHEHLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEKRELSLYASHGVAVLQHGALWQLG"
     gene            complement(1462..2457)
                     /locus_tag="ACHKUC_004046"
     CDS             complement(1462..2457)
                     /locus_tag="ACHKUC_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6620210.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTLHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFYM
                     ATGDDHYTDLESLLNAHFQSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRETRIPCPLLIRCST"
     gene            2681..2905
                     /locus_tag="ACHKUC_004047"
                     /pseudo
     CDS             2681..2905
                     /locus_tag="ACHKUC_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2923..4251
                     /gene="dsdA"
                     /locus_tag="ACHKUC_004048"
                     /pseudo
     CDS             2923..4251
                     /gene="dsdA"
                     /locus_tag="ACHKUC_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(4359..5897)
                     /gene="emrY"
                     /locus_tag="ACHKUC_004049"
     CDS             complement(4359..5897)
                     /gene="emrY"
                     /locus_tag="ACHKUC_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6620211.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVAFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5897..7006)
                     /gene="emrK"
                     /locus_tag="ACHKUC_004050"
     CDS             complement(5897..7006)
                     /gene="emrK"
                     /locus_tag="ACHKUC_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO6620212.1"
                     /translation="MLAVVLFIAFSGAYAYWSMELKDMISTDDAYVTGNADPISAQVS
                     GSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
                     EVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAIQAYKANK
                     ALVMNTPLNRQPQVIEAADATKEVWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSL
                     MAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGKNVVFHGRVTGINMGTGNAFS
                     LLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLA
                     STVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1910 a         1667 c         1653 g         1778 t
ORIGIN      
        1 gcttaaccag gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag
       61 caaaacttca tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgcctcctg
      121 ccgggtggat gaacgatcca aacggcctga tctggtttaa cgatcgttat cacgcgtttt
      181 atcaacatca cccgatgagt gaacactggg ggccaatgca ctggggacat gccaccagcg
      241 acgatatgat ccactggcag catgagccta ttgcgctagc gcctggagac gagaatgaca
      301 aagacgggtg tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca
      361 ccggacacgt ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc
      421 tggctaccag tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag
      481 aaggaatcat gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg
      541 tagtcggggc gaaagaccca ggtaacacgg ggcagatact gctttatcgc ggcagttcat
      601 tgcgtgaatg gactttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt
      661 gggaatgtcc ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg
      721 gaatgaatgc cgagggatac agttatcgaa atcgctttca aagtggcgta atacccggaa
      781 tgtggtcgcc aggacgactt tttgcacaat ccgggcattt tactgaactt gataacggac
      841 atgactttta tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct
      901 ggatggatat gtgggaatcg ccaatgccct caaaacgtga aggctgggca ggctgcatga
      961 cgctggcgcg cgagctatca gagagcaatg gcaaactcct acaacgcccg gtacatgaag
     1021 ctgagtcgtt acgccagcag catcaatcta tctctccccg cacaatcagc aataaatatg
     1081 ttttgcagaa aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg
     1141 atgccgaaca ttacggatta caactcggca caggaatgcg gctgtatatt gataaccaat
     1201 ctgagcggct tgttttgtgg cggtattacc cacacgagca tttagacggc taccgtagta
     1261 ttcccctccc gcagggtgac atgctcgccc taaggatatt tatcgataca tcatccgtgg
     1321 aagtatttat taacgacggg gaggcggtaa tgagtagccg aatatatccg cagccagaaa
     1381 aacgggaact gtcgctctat gcctcccacg gagtggctgt gctgcaacat ggagcactct
     1441 ggcaactggg ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg
     1501 cgtctctctt cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat
     1561 gtcatgtgga agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag
     1621 attatcaaag cccattacgg cgacatcctg cgggatacgt acccccttcg ccagaagaac
     1681 ctgataagcc acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt
     1741 gccagattgg aagtgggcat tgagtaaact ttcgagatcg gtgtagtgat catcacctgt
     1801 tgccatgtaa aattgtttca cctcagccag atctcgtcca gcatcacgcc aggcctgctc
     1861 aaatccctgc cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataacgg
     1921 ttgacgatag cccgccgcga gcaaatgcag tgttgattca tattgtgcag tgtaatcatc
     1981 agggatataa ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc
     2041 accatacaga gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc
     2101 atccggacgg tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat
     2161 attgattaaa aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat
     2221 atcaacagag aaaggagtgg tagcagtgtc ctgtgccagc acggcgagag tcgacggctt
     2281 acgtccttga gcgcgcattt tacgggcgga aagatcagga acataattca gggtctggat
     2341 tgcctgcaat acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg
     2401 ggagactgtc atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc
     2461 caagccgttt gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa
     2521 cgatattctc gacgccctat cacccgacac ggcaatacaa taaaaagtaa caataattcc
     2581 cggacaattg tacccaattt cgcctctgtt ctcgccaacg agtcaccatg taggcaagta
     2641 ggtaaaacat gccaacctgg agaagaagat ataaatatgg atcattgtgc tgccgctgta
     2701 tcccgacatc agcccggaaa ttattgcgat tgctatcggt tccggtgcga ttggctgcac
     2761 gatcgttacg gactcgcttt tctggctggt gaagcaatat tgcggcgcta cgctcaatga
     2821 aacatttaaa tactatacga cagcgacatt tatcgcttca gtcatcgctc tggcgggcac
     2881 attcctgctg tcatttatca tctaagcgca aagagacgta ctatggaaaa cgctaaaatg
     2941 aattcgctca tcgcccagta tccgttggta gaggatctgg ttgctcttaa agaaaccacc
     3001 tggtttaatc ctggcacgac ctcattggca gaaggtttac cttatgttgg cctgaccgaa
     3061 caggatgttc aggacgccca tgcgcgcctg tctcgttttg cgccgtatct ggcaaaagca
     3121 tttcctgaaa cggctgccgc gggggggatt attgaatcag aactggttgc tattcctgct
     3181 atgcaaaaac ggctggaaaa ggaatatcac cagccgatcg ccgggcaatt gctactaaaa
     3241 aaagacagcc atttgccgat ttccggctcc ataaaagcac gtggcgggat ttatgaagtc
     3301 ctggcccatg ctgaaaaact ggctctggaa gcgggtttgc tgacacttga ggatgattac
     3361 agcaaactgc tttccccgga gtttaaacag ttctttagcc aatacagtat tgctgtggga
     3421 tcaaccggaa atctggggtt atcaattggt attatgagcg cccgcatcgg ctttaaagta
     3481 acggtgcata tgtctgctga tgcccgcgcc tggaaaaaag cgaaactgcg cagtcatggc
     3541 gttactgtcg tggaatacga gcaagattat ggtgttgctg tcgaggaagg acgtaaagca
     3601 gcgcagtctg acccgaactg tttctttatt gatgacgaga attcccgcac gttgttcctt
     3661 ggatattccg tcgcaggcca gcgtcttaag gcgcaatttg ctcagcaagg tcgcatcgtc
     3721 gatgctgata accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg tcctggtggc
     3781 gtcgcattcg gacttaaact ggcgtttggc gatcatgttc actgcttttt tgccgaacca
     3841 acacactccc cgtgtatgtt attaggcgtc cataccggat tacacgatca gatttctgtt
     3901 caggatattg gcatcgacaa ccttaccgct gccgatggcc ttgcagttgg tcgcgcatcg
     3961 ggctttgtcg ggcgggcaat ggagcgtctg ctggatggtt tctataccct tagcgatcaa
     4021 accatgtatg acatgcttgg ctgactggcg caggaagaag gtattcgtct tgaaccatcg
     4081 gcactggcgg gtatggccgg gtctcagcgc gtgtgcgcat cagtaagtta ccaacagatg
     4141 cacggtttca gcgccgaaca actgcgtaat gccactcatc tggtgtgggc gacgggaggt
     4201 ggaatggtgc cggaagaaga gatgaatcaa tatctggcaa aaggccgtta ataacgtttc
     4261 aacgcagcat cgccatcctt tccctgggtg agcgatgctg ccgatgacgc agacttaaga
     4321 ttctgccgtt ttacctgcta tagcctcctc tttttccatc acccaacgcc tttcgctgta
     4381 aacggcggtt tcgcaaacca aaccaaaacc gtaagtaaga taaaagcaat agccgccata
     4441 cggaaaatct catttgcaga aatagaaagt gactgctgag ttatttcatt attaatttca
     4501 ttaagaactc ctgaaagcga accatagtat ttatccataa tttgtgacga tgaattaaac
     4561 acggggttaa attgatcgat ggttgctgtc aactgactat ggtgtaacga ttcgcggcgt
     4621 ccccacagtg tcattgtcaa cgacgtacca actgatcctg acaaggtgcg aaaaaaatta
     4681 ctcatactcg aggcattggc aaatttatta tctggcaagc ctgaaaacga aatcgttgtt
     4741 aagggtaaaa agaaacaggc aacggcgaat ccctgaaaaa actgtggcaa aatgatgcct
     4801 gtaaaatcaa tcgttggcat aaatgtcaca gaacgccagt aatagcaaac cgcatacatc
     4861 aaaaaactaa atgccaccaa caaccgcata tcaattttgt tgccataacg tcctatcaaa
     4921 ggtgatatta atagcggcat gataccgatg ggcgcatagg caagtccggc ccatatcgca
     4981 ttatacccca tcgtttcctg gagtaactgc ggcataagaa ctatcgctcc agagtaaaat
     5041 aaatacgcgc atgtgatact cacaatacca atggtgaaat tacgggactt aaacaaactg
     5101 aggtcaagaa tcggattctc tgaggtcgac tcccaaatga ctaaagagat cagagaaata
     5161 actgatacta ctgttagtag tattattgta ctcgagttga accaatccag atcgcgccct
     5221 ttatcaagca taatttgcaa gccaccaaca ccgagcacta acagggtcag tcctggtaga
     5281 ttcattttga ccggtgaagt ctcagtttct cttcctttaa gtaaggttaa gcataatgtc
     5341 aggacgataa tccccatagg gacattgatt aaaaatatcc aaccccagct aaagttatcg
     5401 caaatatagc cgcccaatat cggcccacat atcggagcga taatcacggt cattgaccat
     5461 aatgccagag caaatgttct tttttctggt ggataattcc ttaataataa actctgtgac
     5521 agtggaatta acggccccgc cattaacccc tgaacgactc taaaaaatat cagcacatct
     5581 agattggtcg ataaactaca cattaatgaa gacagcgaaa aaaaggttac tgaaagtaaa
     5641 aataacctta attcgcctat tctttgtgcc agcctgccgg taacagggat cgcaatggca
     5701 tttgctacgc caaacgaggt gataacccag gtgccttcgt ctgttgatgc tcccagaaag
     5761 ccagatattg tcggtattgc gacgttagaa atagtggaat ccaacatttg cataaacgtc
     5821 gctaatgaca atgcaatagt gacgcaccat aacgtcccac cggttaatgg tgcaggagtt
     5881 gatttagtga ttgccattaa agttgtccat tatgcgaaat aatgttgcta atttcttttt
     5941 ctatcggact ggtatcgata actaaagcct tactggtata agcaggcatg gacgtcacgg
     6001 ttgaagccag atcgggcatc tcggcaatgt cttcattctt cgtatcaata gttgcagtca
     6061 tcgataaacc aatgcgcaaa gggtgttcca tgagttcttt tggatccaga gaaacttcaa
     6121 ccggtacacg ctgaacgatt ttgatccagt tccctgtcgc attttgtgca ggtaataagg
     6181 agaacgcatt gccggttccc atattgatcc ctgtcacccg accatgaaac acaacatttt
     6241 taccataaag atcgctgata atattgaccg attgaccaat ccgtacatcc gtgagttgtg
     6301 tttctttaaa gttggcatta acccacattt gacgcgccgg tacgacagcc attaatgatt
     6361 gtccgggact cactgtttcg ccgacctgaa cacttctctg ggcaatataa ccggtaaccg
     6421 gactcttaat atccgtacgt ttaagcgcca accagacttc tttggttgca tctgcagctt
     6481 caatgacttg tggctgacgg tttaatggtg tgttcattac taaagcttta ttcgctttat
     6541 aagcctggat agcagcattc aatgccgctt tgctacttat taacgtatct ttggtatgct
     6601 ccagcgcttc ttttgaaata accccttgct tcgctaaagg cacacgacgg ttataatctt
     6661 ctaaagattg ttgatactga atacgtgctg aagcgacttc ggcactgtat tgtttatcct
     6721 gtaagtatag tttattcgtt tgccgaacaa tatttgccag attattttta gctttattga
     6781 gtgcgatagt ggcatcagtt ttatccagtg aaactaaaat gtcaccttgt cgaacgtagt
     6841 tcgtatcttt atgattaacg acagtgacgc tgcctgagac ttgtgcagaa attggatctg
     6901 catttcccgt gacataggcg tcatctgtac taatcatgtc ttttaattcc attgaccaat
     6961 aggcataggc acctgaaaac gcaataaata aaactaccgc taacaaag
//
LOCUS       DAZFRG010000235         6946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFRG010000235 DAZFRG010000000
VERSION     DAZFRG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.236"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..455
                     /locus_tag="ACHKUC_004051"
     CDS             <1..455
                     /locus_tag="ACHKUC_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO6620213.1"
                     /translation="IGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDD
                     VVILISNSGETAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNL
                     GLAPSCSSTVVLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            527..1474
                     /locus_tag="ACHKUC_004052"
     CDS             527..1474
                     /locus_tag="ACHKUC_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6620214.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1774..3150)
                     /locus_tag="ACHKUC_004053"
     CDS             complement(1774..3150)
                     /locus_tag="ACHKUC_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO6620215.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3150..3578)
                     /gene="tssE"
                     /locus_tag="ACHKUC_004054"
     CDS             complement(3150..3578)
                     /gene="tssE"
                     /locus_tag="ACHKUC_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6620216.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQISERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3582..4118)
                     /gene="tssJ"
                     /locus_tag="ACHKUC_004055"
     CDS             complement(3582..4118)
                     /gene="tssJ"
                     /locus_tag="ACHKUC_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6620217.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4099..5178)
                     /gene="tssG"
                     /locus_tag="ACHKUC_004056"
     CDS             complement(4099..5178)
                     /gene="tssG"
                     /locus_tag="ACHKUC_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6620218.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPDGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5142..6902)
                     /gene="tssF"
                     /locus_tag="ACHKUC_004057"
     CDS             complement(5142..6902)
                     /gene="tssF"
                     /locus_tag="ACHKUC_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6620219.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGIQQFWLRLPGQERRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPERITGFEIDV
                     VLAENWPHDLPFNTDNLRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLNLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1605 a         1817 c         1857 g         1667 t
ORIGIN      
        1 ttattggtgt tggtaagtcc ggtattattg ccaggaaact cgccgcaaca tttgccagca
       61 ccggaactcc ctcgtttttt gttcacggta cggaagctgt acacggcgac cttggaatgg
      121 tggcgaaaga cgatgttgtt attcttattt caaacagtgg tgagacggcg gaaatcctgg
      181 cgacactgcc tagtctgaaa aaaatgggta attatttgat ctcttttacc cgcagtcatc
      241 actcatcgct tgcgataagt tgtgatttat ctgttgaaat ccccgtcaaa agtgaggcgg
      301 ataatttagg tttagcaccg tcatgctcgt cgactgttgt tctggttgtt ggtgatgcgg
      361 tggcgcttgc actctctgaa ttgaaaaaat ttactcgtgc tgatttcggc ttatatcatc
      421 caggaggtgc actcggcatt aaagcaaatt cataatttca aaatataaat acagtaaccg
      481 gtcacgccat cttttggggt ggcgttcttt gaagtgagtg tccgctatga aaaatgttct
      541 ggtcaccgtc cccagctttt cagcacgctg tgtttccgcc agcaagttgc taagagagaa
      601 taattttaat cttattatta agaataatgt ggagcatctg cttaaatcag aatccactgc
      661 attacgagaa tctatttgcg cggtcatcgc cggaaaagat ggttatcagg cggacacgct
      721 atcattgctg cctggtgtga gaataatatc gagattcggg acggggatag acaatattga
      781 tcttcgtgcg gcacagcaat ccggtattgt tgtcaataat gcggttggga ttaattcaaa
      841 tgcggttgcg gaatttatca ttggacttat cttcgcgagc atgagaaata tccctggcag
      901 ctatcatgcg atgcaaaatg gctactgggg tgagtcgcat ggctgtgaat tacaagggaa
      961 acggattggc ctggttggct acggtaatat aggtaaaact ctggcgaaaa ggttatccgg
     1021 ttttgatgtt gagcttttag cttttgacaa acaacccgac tatcaggttg ctgacaaagc
     1081 tggagttcag tttgtatcaa ttgaagatat ctttatgcag tcgcatgtca tcattgttct
     1141 tttgcctttt tcttctgagc tggagaactt cattagtcat aaatatttgt cgatgatgcg
     1201 caatggtgct ctgatcatca atgccgcaag gggaaagttg ctggatgagg gcgctttact
     1261 tcaggtgatt gaagaacgaa atgtgtttgc ggcgctggat gtgtttagct ctgaaccgtt
     1321 ggcgcaattt agcccactgc tacatgccaa aaatatcatt acaacgccgc atatcgcggc
     1381 cgccaccgtt gagtcatacc agcaaaccgg gatacacgtt gcccagtcga taattgatta
     1441 cttcgcagga agggagataa aaaacgtgtt gtgagttcag cgaacaaaga ttttaaatac
     1501 aaaaaaaccg caatttgtag aacacattgc ggtttgtcag cattctaata aattggttgc
     1561 gggggccaca gtcaagaggt taatccaatc ttttgaaact taaggtattc actgttcccg
     1621 aacccaagct atctaccgaa atatacactc attatctgat gtgaatcaac gagtagatcg
     1681 aatggcctgt tcctctcttt ttttgtacac agagactcct ttaaaaaatg gcaaggtcac
     1741 atatccctta ttggtacata aatcccccga tgtttactga ctttgttcca ccttcgtatc
     1801 cagtaaaatg acatagcgat tcaacaactg atttaaccgg gtatcaattt gcgtcaacag
     1861 cgttggcgaa acctctccgg tctgtttttg ctcttccagt tgccgaagca gttcttccag
     1921 cggcggctcc tttaacgact gccggatgcc gtacaccgcc gtttttagtt ccgaaccggt
     1981 cagatatctc cccttacgtt catccagtgc atccagctgc gccgacaggc gctgtagctg
     2041 gctctgcgcc tgagcatagc cattcagtgc ctcagcagac agggcgctca gctcccgctg
     2101 tttgcgccac tgcgcgttca gcgccctgac ctgaggatta tccggccaca gggcatcaag
     2161 ctggcgtagc tggcgatatc cctgctccag tggccagcgg gcaggcaatt tgtttaactg
     2221 ctccagttgc atctgacttg ccgcaattgt tctgtcctga gccagcagcg ccttatcctt
     2281 tacatccagc tttgccagtt cgccgctctc cagcgacagc ggcaacggca tgacagacag
     2341 ttgtattcgc tggattagcg cgtccggctg attaagctgc gaccagcccc ataatcctgt
     2401 tccgccgaga ccggccatca caaccattcc ggccataaat atcggccatg tccgctgccg
     2461 ggagatttca ggggatggaa cgggtggact ggcatcctta tcgttttgct gaacggatct
     2521 ggttgcaaaa accggctgtg gactttcctc tgactttttc ggttgattca tttcccggcg
     2581 gggtaattca gggggagtga tagtggtttc cgctccctga ggcgccagcc gttccagacg
     2641 caatgcggta ttgcgaaact gcccggccag tagatccagt ttcgtcatat gccacagttc
     2701 acactgttgc agcgtggttt cgatggcgga aagatgctgt acacaacggt agatctgcgg
     2761 cagatcctga tactgaatat ccagcgttct cagtgcctgc tgcatttttt ctgtgaccca
     2821 gctgagcagt gatattctgg aatgtacctg taccggccag aagtcagccc agcgctggag
     2881 cagtatatcc agtgaaccga gtccgtctgc catccctgcc agcccctgcc gtctggttat
     2941 cgccagtacg taacagacca gcgtctgtag ttccacgcca ttttgccgga acagcgcgag
     3001 gcagagtttt tccacctgac gccagtccac atccggtcgg gccgggtgag tcagtttact
     3061 catttcagcc tgcagttcac cggattcctt ataagtacag ggatcgccgc ccatgacgaa
     3121 ctggctgata aaattcgcgt tacttgccat cagtacgccc gctcctgttg tttgagatga
     3181 cgcagaacaa ttcgtccttc cggagaaaat acgccatcaa agcgtatcca gccggtatcg
     3241 ggaagctgcg cgtggatcag gtaattaaga tgccccggac ggggctgggg gagtaattcc
     3301 acctgtatct gactcaggcg tggttcataa cgcagcagtg tttcttcaat ctgacgcatc
     3361 aggccatgaa tacccgcagc catcccctga tggatcagac tgagatccgg cagaccgtaa
     3421 tccggcaaat gcgccagcgt gcctgcccgg cagttgataa tgcgttgcat attgtccatc
     3481 acggaaagga taagctgatc ccgctcgctg atctgttcca gcggtaactc gccgctgaaa
     3541 ctgaacgtca gcatttcata taaggaaggt gtcgacatcg gttatttatc cttgagaggc
     3601 agcagtcgca ggtcgcttct catcagttca atcgagcgcg gcttgtcggc ctccagttcg
     3661 tcccttttga tcaccagacg ccagctgtcg cttttttcat cagggtgata aaactgccca
     3721 ataatggcga caaactgcgt ctctttatcc agcggcacat tcagggatgc cgtgccttcc
     3781 ggttttaccc agacggtgtg ttttgccagt acgtccttct ccagcgtctt ctcttcctgc
     3841 ataaacaggg tgtcgtaatc cgccttgtca aaatcttcgc gggttttcag ttgccagaca
     3901 tgcaccatcg tggaaagcgg cgtatcttcc gcatccgtat tcagggcgct gcgggagaag
     3961 aaatcgagat gcagtatttt gatttgtttg tagaaaacgg ctgaggccac gcttttcgat
     4021 tcatccacca ctttctgaat cagcccgcag ccactcagaa caaccgtcag catcgccata
     4081 cagagcaacg ccggacgttt aaaacgaata ggaaacatga gtcatactct ccctgttacg
     4141 aatggaaaaa gcgggtatta atccctgata acgccccaga ctgaccgtca ctgttccggt
     4201 cgcgggcgcg gcaaaggagg cgcgaaggat gccggtacgg ctaatctgta cacgctgttt
     4261 tcccagccgg gcagcaggca acaggctgac gggcagcgtc agttgcagac gggcctgaca
     4321 gcgccagccc agataaacgc gcagcagcac cagcaaatcg ctgtgcagtt gcccgtcggg
     4381 cagccagcca cgtgcttctg cggcatcttc ggtatacagt ttcagcagca actggctgtt
     4441 gacgtcggta ccggtacgcc ccagcagcgt tcgcgttttc aggctgatgc gtgaattttt
     4501 tgacagaccc gcaggggccg ggagaattac aggctgcgga tcgtgaggca ccaccgtcgc
     4561 tttggtcagc ggggcaagca gccctaccag tgccgtgacg ccttccgccg tgcgcgtcgg
     4621 caggcgcatc acgctgagca gggccagaaa acgggatacc ggcgtggcga tatggttctc
     4681 gctgccggga atacccagcc cgatgagtcc cagcagacaa cgggaaatgt catccacggc
     4741 acctgcttca aacgacgccg ggtagttata tttgcgccag atgcggtagt actgagtaat
     4801 aaagcgatgg ttaaaaatat cgagaaacgc catcactgcg tcatgtccgt catgacgctg
     4861 ggtaatgtag tccagatagg cggttggcag cggcgaatcc acgccgtaca ggccgagaaa
     4921 agtggtgcgc accgtggggg gcgcgtcggg attgcccacg tcacgttcaa cgttttttaa
     4981 ctcgctgacc ggaaagccca tacctggatg aggacgaaag cgcaccgcat cggtggcggg
     5041 gctgtcggta ctgcccagcg gcgcgttctg ctggctctgt tccagcaact ggcaaaaacg
     5101 gtaaaaattg attcgcgcaa tatccggggc cagccggtcg gtcagcccgg tacgcgctgg
     5161 ctgtgatgct ctgtccattg caggcacttt cctgtgggtt gcagaattaa ggtcagttga
     5221 gtgaataagt ggatatcggc atacagtgcg aaaaagcggt gcagcagttc accaaacagc
     5281 gtgatatcac cttctccggc aaagccgtta ctgtccagcg tcacctgaat atccacgcca
     5341 cgcagcagaa agcctctttc aaaacgctgt atcaggctgt gctgaacgtc gacaatcgcc
     5401 tccagacgac ggcggttcat ctcgctttcg gtccagtcgt acagtgccag cgtgccgcgc
     5461 agaatttcgg cgttatccat catcgataaa aagttagaac caaggtgact gagcacccgc
     5521 cagtggaagc ggtcacgcgc agggggatag cagggctgcg tcggagcgca caggttgcgc
     5581 acccgcagga cgttctgtga ggcatgaact ggcgtatcga gcagcgtgct ctgtaaagcc
     5641 ttacggggaa gctgcccgtt agtaccggtc aggtttaaag agagcgtttc atcctccagc
     5701 atccggtcag tatcaaacgc atcgccgccc agaatcagcc aggtgtcatg caggccggaa
     5761 gggccgcgct ttacccgcgt gtggtaatag cgttccggcg catcgtggcg cagcatcccg
     5821 ccccggtgac gaaagctgga gaaggggaca tacgcctgac tgccggtatg gcgcgaagaa
     5881 ataatgttgt cgacggaata aatctcggta tgcccgtcct gaatgcgcat cgggcgcagc
     5941 agaaattcgt tttccagcgg cgaaaggtgt aacggatcgg cttcgagcgg gaacaggttg
     6001 atgaccggca cgcagtgcag gcggagattg tcggtattaa acggcaaatc atgaggccag
     6061 ttttccgcca gcaccacgtc aatctcaaag ccggttatcc tttccggcca gatgacgttc
     6121 tccagcccgt tcagcgccac aaacataaac ttctcacgga aggtgaaata ttccagcaac
     6181 aactggtagc cgctgaacgc cgactcgcct ttcggccaca gtcggtcatc gtcatcaaaa
     6241 cccatcgggg aaaagtgggc gtccagcaca cggcgttcct gccccggcag acgcagccag
     6301 aactgctgga tgccaagcgt taaagcccgg tgcagcgcac aggccaccgg gctgtcggca
     6361 ttgagataga gcggcaggcg ggataaatcg atctgtgacc agtcaccaac cagcgggccg
     6421 cattcaaagc gcagacggat aaccgagcgt ccgtccggtt catattgcaa tgacacgtcc
     6481 ggcaacgcca gcggatgcag cgtgatatca cgcgtggcgc tgtagacgca gcgtgttctg
     6541 cgttcgccga tgggacggga tagcacctga aactctttca gcgtatcact ttgcttcatt
     6601 tgctggtgat ccgtggtcag ttcgaccacg gaaagcgacg gaatggtgcg caggtagtgc
     6661 ggccacagca ggctgaccag cccctcggtc agttcgggca ggtcgtcgtc cagcttttcg
     6721 cgcagtcgcc ccattaaaaa agcgaagcct tcgaacaggc gctccacata gggatcgcgt
     6781 gcgccgggtt tatcaagatt gagcatcgcg gcgcggtcgg ggtaagcctg cgcaaattct
     6841 ttccccgctt cacgcaggta gcgcatttcg gcttcgtagt agcgttgggt caggtcgtcc
     6901 atcggaggat ttatcttcct tttattgaaa ttaataccaa cgagta
//
LOCUS       DAZFRG010000236         6915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFRG010000236 DAZFRG010000000
VERSION     DAZFRG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.237"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="ACHKUC_004058"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="ACHKUC_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO6620220.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="ACHKUC_004059"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="ACHKUC_004059"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO6620221.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQKMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="ACHKUC_004060"
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="ACHKUC_004060"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO6620222.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="ACHKUC_004061"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="ACHKUC_004061"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO6620223.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVYLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="ACHKUC_004062"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="ACHKUC_004062"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO6620224.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHKUC_004063"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHKUC_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO6620225.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
BASE COUNT         1736 a         1621 c         1806 g         1752 t
ORIGIN      
        1 taatgagtac agtgataatt aatggcgaaa tgaatgacgc gcattttcca catctttaag
       61 ttgcggttat tacaccattg caaaaactca acgccaggta atgaggccta ttgactggtt
      121 aaaaagaaaa catcccgcat gggtaccaaa gacaacaagg acttccaggc taatcttatg
      181 gattattctg ctgtgcactc gataatttgt ctgacaaatt cgcttttcct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag ttaaaaggta
      361 atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggtttcc tggtgaagca
      541 accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgaa gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgctatcga
      661 cagcctggct gccagtggcg cgaaaggttt cgttatttgt actccggacc cgaaactcgg
      721 ctctgccatc gtggcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggctgcgac
      841 caaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggcgattac ctccaacgaa ctggatacgg ctcgtcgccg
      961 tactacgggg tctatggatg cgctgaaagc tgcaggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat tgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gtatcggtat
     1201 taacggtgtg gatgcggtga gtgaactgtc taaggcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggtta taagtccagc gaaatgcttt acaactgggt
     1321 tgcaaaaggt gttgaaccga ccaaattcac cgaagttacc gacgtggtgc tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggaaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcagaaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggggtg gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgtt cattgctgaa
     1861 ttatgaggca ggtttacaaa ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaagcgc tggcgcgtaa
     1981 cgccaaaatt atcgcctttg atgagccaac cagctccctc tctgcccgcg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcacc gtctttaaag atgggcgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcactggtgc aggcgatggt
     2221 cgggcgagac attggcgata tctacggctg gcaaccgcgt agttatggcg aggagcggct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggagaaatt gtcggtctgt ttggtctggt cggggcaggg cgtagcgaat taatgaaagg
     2401 catgtttggc ggtacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ctattaccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcctg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 caaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatta tgaatctctc
     2701 aggtggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggtgct aagcacgaaa tatataacgt
     2821 gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgcgaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg ttctggagca
     3061 cctaagtcgt cattcagctt tgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgcttt ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttctatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 actgcggtgg ttatcaacct gactgaaagc ctgtggattg gcgtggcagc gggtttgttg
     3361 ctgggcgttc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgcc
     3421 ctcatcacga cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc caactggttt
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtttga tcatctttgg cttgctgctg
     3601 aataaaacca cctttggtcg taataccctg gcgattggcg ggaacgaaga ggctgcgcgt
     3661 ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc tggcctggta
     3721 tcagcgatag ccggaattat tctggcttca cgtatgacca gtgggcagcc aatgacgtcg
     3781 attggctatg agctgattgt gatctccgcc tgcgttttag gtggcgtttc tctgaaaggc
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcacagtacg tagttcgcgg cttaatcctg
     3961 ctggcagcgg tgattttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttccacaac aatttagcgt tttttcccac cacagccagc cgccacaacg gttggctgtt
     4081 cttcgttgca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaac acaataagaa aagagaagga ggagaaccgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatctgcga aatatgcctg gttttttgat ctcgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aagtcgtcgt gcctgacact attctgcaag gacttcagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatttca gggcgctcaa tggtggagct
     4381 tgacgcactg gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacgc atatcgttta tctgccggat gcgattgcgc gtgatattag
     4501 cgtgcaactg catacggtca tcgcccagta tcccggcgcg gagctggagg cgaaagggat
     4561 ggcttttgcg ctgcattatc gccaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg aaaggtacca gtaaaggtga ggcgattgct gcttttatgc aggaagctcc
     4741 ctttatcggg cgaacgcccg tatttctggg cgatgatctg accgatgaat ctggcttcgc
     4801 agtcgttaac cggctgggcg gaatgtcagt aaaaattggc acaggtgcaa ctcaggcatc
     4861 atggcgactg gcgggcgtgc cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagac gagcacgccg ccagtgccgg tggccttgcc gttggcatac
     5041 tgggggcact gaaagccgca ggtggactgt ggtttggctg gagcggtgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaagg gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaaca aattctccaa tgccgttctc tggcccgctt
     5221 ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggatggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccctttgcgc atgaattacg caaacggggc gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacggaga
     5521 acgatcgtct ggcgttcctg gattgccttt ctaacctgac ccgcgtcacg acacgtagcg
     5581 caaaaagcca tacggcctgc ggcaaagcgt ttcgaacgga agtttacccg atcggcattg
     5641 agccgaaaga aatagcgaaa caggctgccg gtccactgcc gccaaaactg gcgcaactca
     5701 aagcagaact gaaaaacgta caaaatatct tttctgtcga acgactggat tattccaaag
     5761 gtttgccgga gcgttttctc gcctatgaag cgttactgga aaaatatccg caacatcacg
     5821 gtaaaattcg ttatacccag atcgcgccaa cgtctcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagccg gacgaattaa tggtaaatat gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtggcaa
     6061 aagagtatgt tgccgctcag gaccctgcta acccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa tccctacgat cgtgacgaag
     6181 ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cagaaagcca gcagcgcgat aaagttgcta
     6361 cctttcctaa gcttgcgtag gtgcggttaa tctcccgtaa gtggaactaa cagcacatcg
     6421 acctcacttg aggtaatcac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatgtttgc gacaaacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg cgtttcttcc tgcatcacat tgcgtaaatc ttcaagcatc
     6661 ggcgcagcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta
     6781 accgcaacgg cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac tcagttaaac
     6901 aggtaaatat tagtc
//
LOCUS       DAZFRG010000237         6859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFRG010000237 DAZFRG010000000
VERSION     DAZFRG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.238"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..623)
                     /locus_tag="ACHKUC_004064"
     CDS             complement(204..623)
                     /locus_tag="ACHKUC_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO6620226.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVELHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
     gene            complement(704..1141)
                     /locus_tag="ACHKUC_004065"
     CDS             complement(704..1141)
                     /locus_tag="ACHKUC_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFO6620227.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGR
                     REFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
     gene            1402..2973
                     /locus_tag="ACHKUC_004066"
     CDS             1402..2973
                     /locus_tag="ACHKUC_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO6620228.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGSGGGGGTGG
                     SKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNE
                     ALRHNSTHLEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKE
                     IEKEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNT
                     RLSSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATR
                     LRARAGDEMEEKQKQVTASETRLNQISSEINGIQKAISQANNKRSTAVSRIHDAEDNL
                     KIAQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALA
                     AFEKYKDVLNKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVS
                     DILKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAF
                     IDKNKLNTINEVLGI"
     gene            complement(2970..3311)
                     /locus_tag="ACHKUC_004067"
     CDS             complement(2970..3311)
                     /locus_tag="ACHKUC_004067"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO6620229.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            3359..3496
                     /locus_tag="ACHKUC_004068"
     CDS             3359..3496
                     /locus_tag="ACHKUC_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO6620230.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            4180..4367
                     /locus_tag="ACHKUC_004069"
                     /pseudo
     CDS             4180..4367
                     /locus_tag="ACHKUC_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4519..4710)
                     /locus_tag="ACHKUC_004070"
     CDS             complement(4519..4710)
                     /locus_tag="ACHKUC_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO6620231.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4777..5100
                     /locus_tag="ACHKUC_004071"
     CDS             4777..5100
                     /locus_tag="ACHKUC_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO6620232.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5309..5743
                     /locus_tag="ACHKUC_004072"
                     /pseudo
     CDS             5309..5743
                     /locus_tag="ACHKUC_004072"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5796..6338
                     /locus_tag="ACHKUC_004073"
     CDS             5796..6338
                     /locus_tag="ACHKUC_004073"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO6620233.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRD
                     AISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVT
                     GGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSG
                     VKGENNWTVEKKNAVRLVRE"
     gene            6345..6578
                     /locus_tag="ACHKUC_004074"
     CDS             6345..6578
                     /locus_tag="ACHKUC_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO6620234.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
BASE COUNT         1777 a         1466 c         1910 g         1706 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagggatt atggtaaatc cacttactgt cttccctcgt agccatcgag ataaaccgca
      241 gaaatcgtct cagccagcag cctggatttt ttcggggtaa ggtctttgct gcggtaatca
      301 gtacccgcaa attgagttgt gccccggagt gagtttaaaa attcctggct gtgaaactgc
      361 tgaatgcgtt gctgcacatc actccgtgat tttcctgtcg ctttatcctt tgctcccata
      421 tcccagaact gccggaaaaa aggcaccatc attcgtgttg tgtcggcttt ttgggtggaa
      481 aagttgggat caaaaccgtc atcagaagca tatacgtaat gccgggtatg tagttccacg
      541 ctgccctttg gggtacaggc tgtgagagct actgttgctg ttatcatggc gataagacaa
      601 agtttgtttc cggtacgtct catgagagtc tccctacctt aaagtattta ttttcggtga
      661 gttattataa gaagactatt ttatcatcag tccatgacag cgttcagcga ccaaacaacc
      721 gggaccagaa tccccgtttc ttttcctgct ccagcgcctg cctgagctgg gctatctcat
      781 tctgtagctg ttcccgttct gcttcctggc gccggcgctc cgtatcctgg gcttgtttat
      841 cctcaagcat cagcgtcagg cattgtttca gctcattcag ctcccggaga atgcgttctg
      901 tctgctgatc atgtggattt tctgcctgtc ccgcactgtg cctttctggt gtctcatcct
      961 gctttaattc gccgtatgcc cggatcagtt cgctggtttc aaactcccgt cgtccgtcct
     1021 ggctggtacg gtaactgaca aggcctgcgc gcatatcacg gtaaaactgt gaacgcgatc
     1081 tgcctgtcat tttcagtgcg tcctggagtg tgtgccatgc catgaagtga cagtgtccca
     1141 ttggtgtctc atatcatggt ttcagtaaaa catactgagg ccggagaacg gtaatgtttg
     1201 tgctggtttt tgtggcatcg ggtggaaata gcacgtggtg tggggggctg tttttttgat
     1261 cgttttcaca aaaatgaaaa tccacagggt tgacagggaa aatgcagcgg cgtaattttt
     1321 atgctgtata taaaaacagt ggttatatat acagtatatt gcttttaact atttgttttt
     1381 aatgtcaaag aggattttat aatggaaaca gctgtagcgt actataaaga tggtgttcct
     1441 tatgatgata aggggcaggt aatcattact cttttgaatg gtaatccaga cgggagtggc
     1501 tctggctctg gcggtggtgg tggaactgga ggtagcaaaa gtgaaagttc tgcagccatt
     1561 catgccacag ctaaatggtc tactgctcaa ttgaagaaaa cgcaggcaga acaggctgcc
     1621 cgagcaaaag ctgccgcaga agcacaggct aaagcaaaag caaaccggga tgcgctgact
     1681 caacatctga aggatattgt gaatgaggcg cttcgccata attccactca tctggaggtt
     1741 attgacctgg ctcatgccaa taatgcagcg atgcaggcag aagcagagcg gttgcgcctt
     1801 gcaaaagcag aagaaaaagc tcgtaaagaa gcggaagctg cggaaaaggc ttttcaggaa
     1861 gcagaacaac gacgtaaaga gatagagaag gagcaggctg aaacagaacg ccaattgaaa
     1921 ctggctgagg atgaagagaa acgcctggca gcattgagtg aagaggcccg ggctgtggag
     1981 gtggcacaaa aaaatcttgc tgctgcacaa tctgagctgg cgaaagtgga tgaagagatt
     2041 aatacgctca atacccgttt aagctccagt attcatgccc gtgatgcaga aacgaatacg
     2101 ctgtccggaa aacgaaatga gttggatcag gcatctgcta aatataaaga actggatgaa
     2161 agggttaaac tgttatctcc tagggcgaat gatccgcttc agagtcgtcc tttttttgag
     2221 gccaccagac tacgggcgag agctggcgat gagatggagg aaaaacaaaa gcaggtaaca
     2281 gcatcagaaa cgcgtcttaa ccagattagc tctgagataa atggaatcca aaaggctatt
     2341 tctcaggcta ataataagcg aagtacagca gtttcacgta ttcatgatgc tgaagataat
     2401 ttgaaaatag cacagactaa tctcctgaac tcgcagatta aggatgctgt ggatgcaaca
     2461 gttagctttt atcaaacgct atctgaaaaa tatggtgaaa aatattcaaa aatggcacag
     2521 gaacttgctg ataaatctaa aggtaagaaa atcagcaatg tgaatgaagc tctagctgct
     2581 tttgaaaaat acaaggatgt tttaaataag aaattcagca aagcagaccg tgatgcaatt
     2641 ttcaatgcac tggagtcggt taagtatgaa gactgggcta agcatttaga tcagtttgct
     2701 aagtacttga agattacggg acatgtttct tttggatatg atgtggtatc tgatatccta
     2761 aaaattaagg atacaggtga ctggaagcca ctatttctta cattagagaa gaaagctgta
     2821 gatgctggag ttagttatgt tgttgtttta ctttttagtg tgcttgctgg aactacatta
     2881 ggtatctggg ggattgctat tgttacaggc attctatgtg cctttattga taagaataaa
     2941 cttaatacta taaatgaggt gttgggtatt taattattct ttattgatat aaacagtcct
     3001 agcaatccaa atgggattgc taggaccaaa caaagtagat tgtatagcat aaataggttt
     3061 aattttgcca cgggggcatt atttaggttt tttctttttc tgaaaaaatc tttctttatg
     3121 aacttaaaaa atatgtgttc aatagcatat tttgagtatg ggcatagaat agtgcttatc
     3181 actattgcgt atagcatctt atctgacgct aggaaataat acccttcgtt gttttttgtt
     3241 ataagatata tatatataag tgcgcagtat aggccaaaca aaatattttt tatgtagtat
     3301 cttaaactca taaattaaac ctcgttatat attcttttca ttttataagg atcgagttat
     3361 gaggaaaaga ttttttgtgg gaatattcgc tataaacctc cttgttggat gtcaggctaa
     3421 ctatatacgt gatgttcagg gagggactgt ggctccatca tcttcttcta aattgacggg
     3481 gatctctgtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3541 atctgctgcc tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa ggttaagtca
     3601 gttggggaac tgcttaaccg aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3661 ccttccagtg tagccgtagt cgggccacta cttcaagaac tctgttgcac catttgcgcc
     3721 atcatcgctc tgctaaccaa gttaccaacg gctgctgcca gtggcgctaa gtcgtgtctt
     3781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgagccgag ataccaacag
     3901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt gtgatgctcg
     4081 tcaggggggc ggagcctatg gaaaaacgcc agcgggcggc cttcttcctg ttcctggtct
     4141 tttgctcaca tgttctttcc ggcgttatcc cctgattctg tggataacag catttcgttt
     4201 ttgagtgagt agataccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg
     4261 aggaagcgga agagcgtcgg aagttgcctt ttctccttac gcatctgtgc ggcatttcac
     4321 atcggctatg gtgcactttt catacaatcc gcactgatgc tgcatagtta agccagtata
     4381 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc taaaacccgc
     4441 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacaggcaag ctgtgaccgt
     4501 ctccgggagc tgcatgtgtc agaggttttc accgtcatcc ccgaagcgtg cgaggcagct
     4561 gcggtaaagc tcgtcggcgt ggtcgtgaag ggcttcgcag atgtccgcct ggtcgtcggc
     4621 gtccagttca ttcagttttt ccagcaggat cagcgtctgg cttctgataa agcgggccat
     4681 gttaagggct gttttttcct gtttagtcac agttctgttc tccatgtgtg gggagtctgt
     4741 cggaggtttt taatgatacc ggtgaaacgc gaaaggatgc tcacgatacg ggtcacggat
     4801 gaggaacatg cgcggctgct ggcacgttgt gagggaaaac aacttgcgtc atggatgcgt
     4861 aaggtctgcc tgggtgcacc gccatcgaaa acgtcaggtc ttccgacact ggcaccgccg
     4921 ctgttgcgcc agttcgcttc ggtcggcaat aacctgaatc agatagcccg gaagataaac
     4981 agcggacact ggtcggggca tgaccgggtt catgtggtgg ctgcactgat ggccatcgga
     5041 cgtgaactga gtgagctgag ggatgaggtc cgtaaacagg gagaacggga tgatagttaa
     5101 gtttcatgcc aggggaaaag gcggtggcag tggtccggtt gattacctgc tgggccggga
     5161 gcgtaaccgg gaaggggcga gaattctgcg gggtgatccc gaagaggtgc gggaactcat
     5221 cgatgccacg ccgtttgcga aaaaatacac ctcgggggtt ctgtcttttg cagagcagac
     5281 actgccaccg ggagagcgtg aaagggtgat ggagagcttt gagcgggtac tgatgcccgg
     5341 tcttgaaaaa aatcagtaca gcatcctgtg ggtggagcac caggacaagg ggcgtcttga
     5401 gctgaatttt gtcatcccga atatggagct ggtgagcgga aaacggctcc agccctatta
     5461 cgaccgtgcg gaccgtccgc gaatcaatgc ctggcagaca ctggtgaatc atcattacgg
     5521 gctgcacgac ccgaacgcac cggagaacag gcggacactt gtcaccccga ataatctgcc
     5581 gaaagcgaaa caggaggccg cagaagcgat tacgaggggg ctggaggctc tttatcatgc
     5641 cggagcgcta aaaacgcgtc aggatgtcac tgaggcgctt acagcggcag gttttgaggt
     5701 ggtgaggaca accaggagca gtatcagtat cgccgacccg gagggcgggc gtaatctcag
     5761 gctgaaaggg gcgctgtatg agcaatcttt tacaaatggg gacagacttc gagaagaaac
     5821 tgaaagagcg tgccgtctct acagagaaca tgctgaacag cgagttcagg aagctcgaag
     5881 cgtctgtaga cagggaactg aacttaaacg ggcagaaaat cagagacgct atcagcgcac
     5941 acacagcagc gctgaaagaa caactggaga cgctgaacag tgccgtgaac gcgcagttca
     6001 gcgccacaga gaacgaactg gcccggcggc aggaagagct gctctggaag ctgatcaaag
     6061 ggcggatact gtacccgtcg ctgacggcgc tttgcgtcac gggcgggatt tttctggcgt
     6121 catgggggct gattcagtgg caggaaagcc ggattgcggc gaacatcctg gctatcaggg
     6181 accaggaaga gacgctggcg aaactccggg aaaaaacatg gggtgtgacg tatcacgagg
     6241 gcaggaacgg gaaatttctg gtattgccgt ccggggtgaa gggggaaaac aactggacgg
     6301 tggagaagaa gaacgcagta agactggtca gggagtaagt gagtatgaca gagctggaaa
     6361 tgcacttact gaacgcctta gagcaattgc agcaggacta tacgcagcgg ctgaaagact
     6421 gggagagcgc cttcacggat ttgcagcata tgttcgagct gacacgacag gagaacgggc
     6481 aactgaggga acagtgcgac gacttgagtc agcaggtgca gagattagcc gggcaggtgg
     6541 accgcttgag tcggttattt atcacgaaca gcagataagg gatgaacagg aaaggctggc
     6601 acgcctggaa cgggagcgtg cgcggtcctg gcatgaagag caggaacggg aagtacgggt
     6661 atggcgtggc ccgtcaatgg ggatgtgacg gtgagttgtt ccgggaggtt gccagatggt
     6721 accggaacgc gttaaaaatg catccgggcg cgattctggc gtgtcgtttt tgttgctgtg
     6781 ttttgcctct ttgccagatc gcgctcattc ctcagcctca tcccgcgatg tctgcgtatg
     6841 tcatcaactg aaaagatgg
//
LOCUS       DAZFRG010000238         6840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFRG010000238 DAZFRG010000000
VERSION     DAZFRG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.239"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..905
                     /gene="chuT"
                     /locus_tag="ACHKUC_004075"
     CDS             3..905
                     /gene="chuT"
                     /locus_tag="ACHKUC_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HFO6620235.1"
                     /translation="MTRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWTFSVT
                     AAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEGILSL
                     RPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVPEQGE
                     ALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAGAENV
                     ATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRIITVDQ
                     NLILGMGPRIADVVESLHQQLWPQ"
     gene            925..2262
                     /gene="hutW"
                     /locus_tag="ACHKUC_004076"
     CDS             925..2262
                     /gene="hutW"
                     /locus_tag="ACHKUC_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO6620236.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEREAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            2275..2769
                     /gene="hutX"
                     /locus_tag="ACHKUC_004077"
     CDS             2275..2769
                     /gene="hutX"
                     /locus_tag="ACHKUC_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO6620237.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            2769..3392
                     /gene="chuY"
                     /locus_tag="ACHKUC_004078"
     CDS             2769..3392
                     /gene="chuY"
                     /locus_tag="ACHKUC_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO6620238.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3441..4433
                     /gene="shuU"
                     /locus_tag="ACHKUC_004079"
                     /gene_synonym="chuU"
     CDS             3441..4433
                     /gene="shuU"
                     /locus_tag="ACHKUC_004079"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO6620239.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4430..5200
                     /locus_tag="ACHKUC_004080"
     CDS             4430..5200
                     /locus_tag="ACHKUC_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO6620240.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5252..5899)
                     /locus_tag="ACHKUC_004081"
     CDS             complement(5252..5899)
                     /locus_tag="ACHKUC_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO6620241.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(5963..6289)
                     /gene="hdeB"
                     /locus_tag="ACHKUC_004082"
     CDS             complement(5963..6289)
                     /gene="hdeB"
                     /locus_tag="ACHKUC_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO6620242.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6405..6737)
                     /gene="hdeA"
                     /locus_tag="ACHKUC_004083"
     CDS             complement(6405..6737)
                     /gene="hdeA"
                     /locus_tag="ACHKUC_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO6620243.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1701 a         1730 c         1684 g         1725 t
ORIGIN      
        1 tcatcaccag gccgtttctt ttttccccac taacgttatg tatttcagcc gtggtctctg
       61 ccgcgaagac catggtaaaa cgaaaaaaat tgtttacggc attactggct cttagctgga
      121 cttttagcgt aacggctgcc gaacgtatcg tggtcgcagg aggatcgctg acggagctaa
      181 tctacgcgat gggcgctggc aaacgcgtgg ttggtgtcga tgaaacgaca tcctatccac
      241 cagaaaccgc caaactgccg catattggtt actggaaaca actcagcagc gaaggcattt
      301 tgtcacttcg cccggatagc gtaattacct ggcaggatgc aggaccgcaa attgtgctcg
      361 accaactgcg ggcgcaaaaa gtcaacgtcg tcactctgcc gcgtgtacca gccacccttg
      421 agcagatgta cgccaacatt cgccaactgg caaaaacgtt acaggttcct gaacaaggcg
      481 aggcgctggt gacacagatc aaccaacgcc tggagcgagt acagcaaaac gtggcggcca
      541 aaaaagcccc ggttaaagcc atgtttattc tctccgcagg cggaagtgcg ccacaggttg
      601 ccggtaaagg cagcgtcgcg gacgccattc tttcgcttgc cggagcagaa aacgtcgcta
      661 cccaccagca gtacaaaagc tacagcgcgg aatcgctgat tgcggctaac cctgaagtga
      721 ttgtcgtcac ctcacaaatg gtcgatggcg atattaatcg gctacgttct attgccggaa
      781 ttacccacac tgccgcatgg aaaaaccagc gcattatcac cgttgatcaa aacctgattc
      841 tgggaatggg tccgcgtatt gctgatgtcg ttgagtcttt acaccagcag ctttggccgc
      901 aataaatgga tttaaatcag acgaatgaac acaaataaca ccctggatct cacgccacat
      961 tttgctttag acggtgatca gccctttaaa gacaggcgcg ccatgatgcc gtttcgtggg
     1021 gccattccgg ttgccaaaga gcaactggca cagacctggc aagagatgat caatcaaacg
     1081 gtatcaccac gtaagcgact ggtttaccta cacattcctt tttgtgcgac acactgcacg
     1141 ttttgtggtt tttatcagaa ccgttttaat gaagatgcgt gtgctcatta taccgacgcc
     1201 ctgattcgtg aaatagaacg ggaagcagac agcgtattgc atcagtcggc ccccatccac
     1261 gcggtctatt tcggtggcgg tacgccttcc gcgctttcgg cacacgattt ggccagaatc
     1321 atcaccacat tacgagaaaa gctgccactg gcccctgatt gtgaaatcac tatcgaaggc
     1381 cgggtactga attttgacgc tgagcgaatc gatgcttgcc ttgatgctgg agcaaaccgc
     1441 ttctcaattg gtattcagtc gtttaacagc aaaatccgca agaaaatggc ccgcacctcg
     1501 gatggcccaa ccgctattgc gtttatggaa agcctggtca aacgcgaccg tgccgcggtg
     1561 gtctgtgacc tgctgtttgg tctgccgggt caggatgcgc aaacctgggg agaagatctg
     1621 gctattgccc gcgatatcgg tctcgacggc gtcgatctct atgcgctcaa tgtcctgccc
     1681 aatacaccgc tgggcaaagc cgtggaaaat gggcgtacta ccgtgccctc tccggcagaa
     1741 cgtcgcgatc tttacctgca agggtgtgat tttatggacg atgccggttg gcgctgcatc
     1801 agtaacagcc actggggccg taccacacgc gaacgcaatc tctataacct gctgattaaa
     1861 caaggtgccg attgtctggc ctttggttcc ggagccggcg gttcgattaa tgggtactcc
     1921 tggatgaacg aacgcaatct tcagacctgg catgaatccg tcgcagcagg caaaaaaccg
     1981 ctgatgatga tcatgcgtaa cgccgaacgt aacgcgcagt ggcgtcatac cttgcagtcg
     2041 ggcattgaaa cggcgcgtgt accgctggac gaacttacgc cacatgccga aaaactcgcg
     2101 ccgttactgg cccagtggca ccaaaaaggc ttaagccgcg atgcgtcaac gtgtctgcgg
     2161 ttgaccaatg aaggtcgttt ctgggcaagc aacattttgc agtctcttaa tgaactgatt
     2221 caggtactta atgcgccagc aattgcgctt gaaaaaccat aacaggagtg atttatgagc
     2281 catgtctcgt tacaggaatt tttgaaaacg gagccagacg gtacgctgga ggcgatcgcc
     2341 gaacaataca acaccacgct gctggaggta gtgaaaaatc tgccctctcc gactgttgtg
     2401 tcaggtgata aattcgatac tgtctgggat accgtctgcg aatggggcaa cgtcaccacg
     2461 ttagtacata ctaccgatgt aatactcgaa tttagcggcg aactgccttc gggtttccat
     2521 cgccacggct atttcaacct acgcggaaag cacggcatgt ccgggcatat caaagcagaa
     2581 aactgcacgc atattgcctt gatcgaacgt aaatttatgg gcatggacac cgcctctatt
     2641 ctctttttca ataaggaagg cagcgcaatg ctgaaaatct tcctcggtag ggatgatcat
     2701 cgtcagctcc tgagcgaaca agtcagcgcc ttccatgctc tggcagcatc gttaaaggaa
     2761 cacgcctgat gacaccgtgg ctactctttg gcgcaggcgg aaaaggtgtg ggtgccagaa
     2821 cgcttgaact ggcgctggcg gaacaacgtc cggtcgttgc tgtcattcgt catgccgatg
     2881 ccgccacgaa actggcgcaa caaggcgtac aggtttttac aggcgacgcc tgtgatgcca
     2941 gcgtagtcgc ggctgcttgc cgcgcagctg gcccggatgc gcttatcatc tcaacaatgg
     3001 gcggagctca ggattatctg gcgcacagaa cagtgattga tgaggccgag aaggctggca
     3061 tcagtcgcat gattctggtg acctctttgg gatgcggcga tagctggccg tttttatcag
     3121 agcgggcaaa ggctgcgttt ggtcaggcgg tacgggaaaa aacgctggcg gaaagctggt
     3181 tgcaaaccag ccagcttgat tatgccattc tccgccccgg cggtctgctt gatggtgcgg
     3241 caacgggcaa agcacaacgg atacaaaatc aggagtgtca tggttttgtt aaccgtgctg
     3301 atgtcgccgc acatatccat gaactggcaa atgcgccagc acttaaccag caggtctaca
     3361 gtctgattga accggacctg aaaccggcgt gatcccccgg cgcggcgtgt ccgcgccgta
     3421 atgtcgtaac ggagttttgc gtgctcaagg attcactctc ttccgccagg gtctttatgg
     3481 ggctatcact attattgcta gctctggtgc tgtttggtgc cagtcaggga gcgttaaaga
     3541 tcagttttga tgcccttttt gatgaggaat accgcgatat ctggctcaat attcgtctac
     3601 caagggtttt gctggcggtg ctggtaggtg cagcgttggc aaccgcaggg gtaattatgc
     3661 aagggctttt tcgcaaccca atggctgacc ctggattact tggcgtcagt agcggttctg
     3721 cattaatggt tggcgttgct attgtgctgc ccttctcctt cccagtggtg ctggtgcttt
     3781 atgagcaaat ggtgtttgcc attgccggaa gtttagtggt ctgcaccatc atttttctca
     3841 tcacgcagcg ccatcgcgat ggcagcatga tgcaattatt actcgccggt atcgccatca
     3901 atgccctgtg cggcgcagcg atcggcatcc tgagctatat cggcgatgag cagcagctac
     3961 gacaactcac attgtggatg atgggcaatc ttggacaggc gcaatggccg acgttattgg
     4021 ttgccagttc attcatccta ccggccatta tcgcaacaac ttgtctcgcc ggaacgctga
     4081 atttactgca gctcggtgat gaagaagccc actacctcgg cgtgaacgtg aagcgtaaac
     4141 gccagcaatt actgttagtg agctcactgc tcgttggtgc tgccgtatcg gtaagcggca
     4201 ttatcggctt tattggcctg gtgatcccgc atctgattcg catgactacc ggggcaaatc
     4261 accgctggct aatcccttgt tccgccctcg ccggagcctg tttattgctg atggcagaca
     4321 cgcttgcccg cacgctggta cagccagcag aaatgcccgt gggattatta accagcctgc
     4381 ttggtggccc ttattttatg tggttgattc tgcgcaaccg gaggatcaca tgatcagcgc
     4441 ccagaatctg gtttatagcc ttcaggggcg gcgtctgact gataatgtct cgctgacttt
     4501 tcccggtggg gaaattgttg ccatcctcgg gcctaatggt gcgggaaaat ctacgctgct
     4561 tcgccagcta accggctatc ttcaacctga tagcggcgaa tgtcggttgt ttaataagcc
     4621 attaaatgaa tggtcgatta ctgaactggc gaaacaccgg gccgtgatgc gtcaaaatag
     4681 ccacatggcg tttccattca gcgtacaaga agttatccag atggggcggc atccgcatcg
     4741 tactggtaat caggataatg aaacagcaca aataatggcg ttatgtgact gtcaggcgtt
     4801 agcaaaccgc gactatcggc aattatccgg tggtgaacaa cagcgcgtgc aattagcgcg
     4861 cctgctagtg caactctggg aacctactcc ctcaccgaaa tggttatttc ttgatgaacc
     4921 aacttcggcg ctggatattc accatcagca gcacttgttt cgtttattac gccagttagt
     4981 tcacgaacgg cagttcaatg tttgctgcgt attgcacgat ctaaatcttg ccgcgcgtta
     5041 tgcagaccgt gttgtattaa tgcaaaaagg aaaggttatc gcaaatggta agccgcagga
     5101 cgtattgaca cagcaggcgc tgacaatgct ctacggcgcg gatattaccg tacttaaaga
     5161 tcctgccaac cattcaccac tgatcgtcct cgatcattaa aaccaacagg agtaccgcac
     5221 tggtactcct gcgaataaat aactatacag attagctaat tgaaacgcct ttcacccctg
     5281 ccataccttt taataatcgc aacagatctt ctatcgacgt cgtggcatga agctggatat
     5341 caatggcgac cacttcatga tcttcatttt cctgcaacga aactaaatcc gtttttattt
     5401 tttgctgctt aaaccagtcg agcattgata ccgtattacc gttcactaat gtcagctgta
     5461 aatggtaatt tttgttcatc aggcggaagg ttaattgatg gaagacttcc agcaccagca
     5521 aggtcatcac tgaaccataa atacccagtt cgtacatacc gctgccaata accataccaa
     5581 tggcggcggt cacccagata tccgctgccg tcgtcagacc tacaatattt tggttacgaa
     5641 cgagaatgtt acctgcaccg ataaacccga cgcccgtcac aacctgagca gcaatacggc
     5701 tgggatccag tccgacgtga cctaaagaca gcacgtcagc aaaaccatat ttcgaaacaa
     5761 tcataaacag ggcgcttccc atgccaatta atacatgtgt gcgtaaccct gctcctttgc
     5821 cgcgcatttg cctttccatg ccaatagcgc cacaggcaat taccgcaagt ataagtcgta
     5881 taataaattc tgctgtcata taaaaatgca aaaaggagca gcaagatggc tcaacttgct
     5941 actccttttt acttgtacct tattaattcg gcaagtcatt agatgcttga tttttgaagg
     6001 tatacaaatt tttctgcggg tttttcttgc agtattcgat cactttagga atttgagtga
     6061 gatcggtttc atttaaagta acggtatcgc cacctttata tactgtttct tcatgcagca
     6121 tccaccacgc aactggtgtc attgcttttg gattcagatc aataaattcc tggcaggtca
     6181 tatctttagc ggattcattg gctgccagcg cagattgtgc gttcaccagt gacaaagccg
     6241 ctacagcgcc cataaaaata aacgctttac ggagagatga aatgttcatt ttgtaaccca
     6301 ttcaatatag agattatata caaacaggag gaatagctgc ttttaaagaa gatattccgg
     6361 cctgttcaat atgcaaggaa atacgatgta acgcattccc ggaattacat atctttctta
     6421 attttgtccc attcgccttt aactttatct ttaaagctgg cttttttatc ctgagtacaa
     6481 gcctgaacga tagctggggt tacggttgca ataccctgaa catctaaaac cgcatcttct
     6541 ggtttatctt tgttgttcag tgcttcagca aaaccaactg cagttggctg gaaggattcg
     6601 tccacagcca ggaaatcttc acaggtccag gagttgaccg gttttttgtt atcagctgct
     6661 ttttgtgcat ccgctgcatt gctcactact ggcagaagaa gcagaccacc aagaataacg
     6721 cctaatactt ttttcatcgt aatatcctca actataaagt gaaagagccg tcacgaatca
     6781 atttcgacac tgaggttata acctggtttt ctgtatatgt catgttgatg gaaaatatca
//
LOCUS       DAZFRG010000239         6821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFRG010000239 DAZFRG010000000
VERSION     DAZFRG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.240"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..990
                     /locus_tag="ACHKUC_004084"
     CDS             <1..990
                     /locus_tag="ACHKUC_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6620244.1"
                     /translation="IFLALVNKVSENTKQRNLFSKKVTLCITLFFVLGGPIVAHIFSS
                     HYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRCDIWNGVSVIA
                     FCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALRVTKDIAHRDP
                     LTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNI
                     IGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGDNPEYAIPQKV
                     TISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILINRKDDE"
     gene            complement(1095..1182)
                     /locus_tag="ACHKUC_004085"
     tRNA            complement(1095..1182)
                     /locus_tag="ACHKUC_004085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1146..1148),aa:Ser,seq:gga)
     gene            1416..2354
                     /gene="ghrA"
                     /locus_tag="ACHKUC_004086"
     CDS             1416..2354
                     /gene="ghrA"
                     /locus_tag="ACHKUC_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO6620245.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2409..3146
                     /gene="ycdX"
                     /locus_tag="ACHKUC_004087"
     CDS             2409..3146
                     /gene="ycdX"
                     /locus_tag="ACHKUC_004087"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO6620246.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3170..3724
                     /gene="ycdY"
                     /locus_tag="ACHKUC_004088"
     CDS             3170..3724
                     /gene="ycdY"
                     /locus_tag="ACHKUC_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO6620247.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3826..4317
                     /gene="ycdZ"
                     /locus_tag="ACHKUC_004089"
     CDS             3826..4317
                     /gene="ycdZ"
                     /locus_tag="ACHKUC_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6620248.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(4381..5214)
                     /gene="csgG"
                     /locus_tag="ACHKUC_004090"
     CDS             complement(4381..5214)
                     /gene="csgG"
                     /locus_tag="ACHKUC_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO6620249.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5241..5657)
                     /gene="csgF"
                     /locus_tag="ACHKUC_004091"
     CDS             complement(5241..5657)
                     /gene="csgF"
                     /locus_tag="ACHKUC_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO6620250.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     EIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5682..6071)
                     /gene="csgE"
                     /locus_tag="ACHKUC_004092"
     CDS             complement(5682..6071)
                     /gene="csgE"
                     /locus_tag="ACHKUC_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO6620251.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6076..6726)
                     /gene="csgD"
                     /locus_tag="ACHKUC_004093"
     CDS             complement(6076..6726)
                     /gene="csgD"
                     /locus_tag="ACHKUC_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO6620252.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECCFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1753 a         1527 c         1666 g         1875 t
ORIGIN      
        1 atatttctgg cattggtaaa taaagttagt gaaaacacaa aacagcgaaa tttgttttcc
       61 aaaaaagtga ctttgtgcat aactttgttt tttgttcttg gggggccgat agttgctcac
      121 atattttcta gccattatga gagctacgat ttacacattg ccgagttaac caatgaaaat
      181 ggtcaggtgg tttggaaagc ctcatatgtt accataatga ttttcatgtg gttaaccttg
      241 ctttcggtta atctatactt taatggttta cgctgtgata tctggaacgg tgtgtcggtg
      301 attgcttttt gtgccgtgct gtacaatatc tctctgttat ttatgagcag gtatagcgtg
      361 tccatttggt atattagtcg cactattgaa gtagtcagta agttaaccgt tttggtaatt
      421 tttatgtgcc atatattcag tgcgctacga gtaacaaagg acattgcaca tcgcgatccc
      481 ttaaccaata tatttaacag aaattatttt tttaatgaac tgacggttca atcagcatcg
      541 gccaaaaaaa caccttattg cgtcatgatt atggatattg accacttcaa aaaagtcaac
      601 gacacctggg gacatccggt tggcgatcaa gtgataaaga cagtggtgaa tatcattggg
      661 aaaagcatac gaccagatga ccttttagcg cgcgttggcg gcgaagagtt tggcgtcttg
      721 ctgacggaga tcgatactga atgtgcgaaa gctttagcgg aaaggattcg ggaaaatgtt
      781 gaacgtttaa ctggcgataa tcctgaatac gccataccac aaaaagtgac gattagtatt
      841 ggcgctgttg taacgcagaa aaatgagtta aaccctaagg agatctatca actggctgat
      901 aacgcacttt acgaggcgaa agagaccggg cgtaataagg tggtagtaaa agaggcagaa
      961 attcttatca accgcaagga tgatgaatga gtctgtcgcc agttgtcgaa ccccgatcgg
     1021 ggcttctcat ccccccggtg tgtgcaatat acgaaaaaaa agcccgtact ttcgtacgag
     1081 ctcttcttta aatatggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca
     1141 cactttccag gcgtgctcct tcagccactc ggacacctca ccaaattgtc gttcctgtct
     1201 tgctggaacg ggcgctaatt tagggaaatc atgacctgag gtcaacaaac ttttttaaaa
     1261 aatcgctcga ttattcaaac ttcaatcaat atgcagtttt aataagcgaa atttgctttt
     1321 tttgccaccg accacggatt tgttatgctg gtggcttttg tagatcataa cgataagtgc
     1381 gaataaattt cgcgcaacgc ttttcgggag tcagtatgga tatcatcttt tatcacccaa
     1441 cgttcgatac ccaatggtgg attgaggcac tgcgcaaagc tattcctcag gcaagagtca
     1501 gagcatggaa aagcggagat aatgactctg ctgattatgc tttagtctgg catcctccag
     1561 ttgaaatgct ggctgggcgc gatcttaaag cggtgtttgc acttggggca ggtgttgatt
     1621 ctattttgag caagctacag gctcaccctg aaatgctgaa gccttctgtt ccactttttc
     1681 gcctggaaga taccggtatg ggcgagcaaa tgcaggaata tgctgtcagt caggtgctgc
     1741 attggtttcg acgttttgac gattaccgca tccagcaaaa tagttcgcat tggcaaccgc
     1801 tgcctgaata tcatcgggaa gattttacca tcggcatttt gggtgcaggc gtactgggca
     1861 gtaaagttgc acagagtctg caaacctggc gcttcccgct gcgttgctgg agtcgaaccc
     1921 gtaaatcgtg gcctggcgtg caaagctttg ccggacggga agaactctct gcatttctga
     1981 gccaatgtcg ggtattgatt aatttgttac cgaatacacc tgaaaccgtc ggcattatta
     2041 atcaacaatt actcgaaaaa ttaccggatg gcgcgtatct cctcaacctg gcgcgtggtg
     2101 ttcatgtcgt ggaagatgac ctgctcgcgg cgctggatag cggcaaagtt aaaggcgcaa
     2161 tgctggatgt ttttaatcgt gaacccttac cgccggaaag tccgctctgg caacatccac
     2221 gcgtgacgat aacaccacat gtcgccgcga ttacccgtcc cgctgaagct gtggattaca
     2281 tttcgcgcac cattgcccag ctcgaaaaag gggagagggt ctgcgggcaa gtcgaccgcg
     2341 cacgcggcta ctaataaagc atctggtttc ctgctatcct tggcgggaat tgaatacagg
     2401 agagagttat gtatcccgtc gaccttcata tgcataccgt tgccagcacg catgcatata
     2461 gcacattgag tgattacatt gcccaggcca aacaaaaggg cattaaactt tttgcgatca
     2521 ccgatcatgg cccggatatg gaagatgcgc cgcatcactg gcacttcatt aacatgcgta
     2581 tctggccgcg agtggttgat ggggtaggga tcctgcgcgg catcgaagct aacattaaaa
     2641 atgttgatgg tgaaattgat tgcagcggca aaatgtttga ttcgctggat ctgattattg
     2701 ccggttttca tgagccggtt tttgcgccac atgacaaagc gaccaataca caagcgatga
     2761 tcgccactat cgccagcggc aatgtgcata taataagcca tcccggaaac ccgaaatatg
     2821 aaatagatgt taaagccgtt gctgaagcag ccgcgaaaca tcaggtggcg ctggaaataa
     2881 ataattcctc ttttttacac tcacgtaagg gaagtgaaga caattgtcgt gcggtagccg
     2941 cagcggtacg tgacgctgga ggttgggtgg cattaggctc ggattctcac accgcgttta
     3001 ccatgggtga atttgaagag tgtcttaaaa tcctcgacgc ggtagatttt ccgccagagc
     3061 gcattttgaa tgtttctccg cgccgcttac ttaacttcct tgaatctcgc ggtatggcac
     3121 cgattgcgga atttgcagac ctttaattac tagtaatgga aatatattaa tgaacgagtt
     3181 ttctatcctc tgtcgtgtgc tgggttcgct ctattaccgc caaccacaag atcctttact
     3241 ggtgccgctg tttaccctga ttcgtgaggg gaaactggct gcgaactggc cactggagca
     3301 ggatgagttg ctgacacgtt tgcagaaaag ctgtgatatg acgcaagtct ctgccgatta
     3361 caatgcgttg tttatcggcg atgaatgtgc tgtgccgcca tatcgtagcg catgggttga
     3421 gggcgcaacg gaagcggaag tgcgcgcttt tctttccgaa cgagggatgc cactagcgga
     3481 tacgccagcc gatcacatcg gcacattgct gcttgcggct tcctggctgg aagatcagtc
     3541 aacggaagat gagagcgaag cactggaaac actgttcagt gagtatctgt taccctggtg
     3601 tggtgcgttc cttggcaaag tggaggccca tgcaactacg cctttctggc gcaccatggc
     3661 accgctaacc cgcgatgcta ttagtgcaat gtgggacgag ctggaagaag attctgaaga
     3721 gtaaatgtga tctgtatcac tcataactgc aacgagtaat ttgttgttgc ataaaatgtg
     3781 tgctcgatct cattcatggc cgcgttttct gctatcatgc gcggcatgaa catacttctc
     3841 tctattgcaa tcacaacagg cattctctcc ggtatctggg gatgggtggc tgtttccctg
     3901 ggcttactaa gctgggcggg cttcctgggc tgtacggcct actttgcctg cccgcaaggt
     3961 gggctgaaag ggctggcgat ctccgctgca accctgctta gtggcgtggt gtgggcgatg
     4021 gtcattattt acggtagtgc attggcacca catctggaaa tactcggtta tgtaataacc
     4081 ggtattgtcg cgtttctgat gtgtatccag gcgaaacagc tgctgctttc atttgttccg
     4141 gggacgttta taggcgcatg cgcgacgttt gccgggcagg gtgactggaa actggtgtta
     4201 ccttcgctgg cgcttggact ggtatttggt tacgcaatga aaaacagcgg cctgtggttg
     4261 gcggcgcgta gtgcaaagac agctcaccgt gagcagcaaa tcaaaaataa agcgtgaggg
     4321 gcactcacgc tttcgcttaa accgtaaaat gccggatgat aattccggct tttttatcta
     4381 tcaggattcc ggtggaaccg acatatggcg gtatttcacc agaatgtcat tctgccgttc
     4441 tgctttattt tgcaaatccc acagaccacg gtcgatacca tcattaatca ggaaaatgac
     4501 ccctgtttcg atagccgaca tcaggcacag cataacaggt tcgttcgagg tgtaacccac
     4561 ttccccttcc agcaagcgct ggtagtcaat aaagcggaaa accccggcct gaacttcata
     4621 agaaagtatc gtcttactgg tgttcaccga ggagaggatc tcaccggtac tcacattgac
     4681 gacgcgcagg ttcacggcaa tctgatcgag ctggtattgc gtgtcggcac cgattccaaa
     4741 atatcttgcc ccaaccccgc ccgatttgac gttgctttca taaccgataa tcgaaccttc
     4801 aaccatgata tttgccgccg ttaaagattg cagcgggatt cggttattaa tggcaactgt
     4861 gccgttttct tgtgccgcac gaataatctt gcgctcgtta agcagatttt gtaagccctg
     4921 gcgctccagc ggtataaacc agcgagaatc tttcaatgcc gtgaccagca ttgccgtggc
     4981 gctttgcgga acagcagtgg agaagttgct tgccgggtag ggtttaaatt gcccggtttc
     5041 gtcctgaatg ttgtataccg aaacaaagat cttacccgtc ggcgctggca gatgggtcaa
     5101 atctttgtag ctctgagcac gaggcattaa tgtcggtctg gcggcttctt taggcggggc
     5161 ggttaagcat ccgctcagta acatgacggc aaccaaaaga aataagcgct gcatgattat
     5221 tttccttatg aagctggggc ttaaaaatcg gttgagttat tttgtaaacc cgaaacctgg
     5281 atggtcgagg tttgtccggt tttacgatct gtcacgttca actgcaattg accatcgcgg
     5341 ttagcaatct caacaatata atcgttggtc accatgcggc ccggtttacc ggtattaata
     5401 ttcgacagta gcccacccaa aatttgtgac tggatggcct gagtaaagtt atctaacgcc
     5461 gagggcgttt caataccaaa gtcatcgtta tagctcggat ctttataaga gttttgggcc
     5521 tgagcgctat ttaacaaaaa agcgccatta tttgggttac caccaaagtt tggattacgg
     5581 aactggaaag tcatggttcc agcccaactt aatggcgaaa taagcatgag tagaactact
     5641 gcatgtttga cacgcattgc agcctccgaa caatttatta tttaaaattc atcatgcgcc
     5701 aaatcgcccg tacttaataa cgcctggttt atctggcgac gatttagtgc ttcttcagtt
     5761 tgaatcagtg caaagacgac agttttctcg aagtctcttt tcaacggaaa taaaaaagtc
     5821 tggaaaataa cgtcctgatt caccgttatt gtgatccagc ttccccatcg tgcactgggc
     5881 ctttcattaa tcgttaagtt acccgtatag tcactttccc atttatcact aaaggctcgg
     5941 taaaaatcat ggccaataga tgaaacagta tggtcagtta acaatcccgg gacttctacc
     6001 tcaacggcgt gaagattccc tgcggcgaac agaaattctg ccgccacaat ccagcgtaaa
     6061 taacgtttca tggctttatc gcctgaggtt atcgtttgcc caggaaaccg cttgtgtccg
     6121 gttttttacg gctatcttct tgaaaagatt ataaagatgc gttttaaccg tattttcgct
     6181 gatgaacaac gaacgagcga tctcgttatt agacgcgccg atacgcagct tattcaggat
     6241 ctctttttcc cgatgagtaa ggagggctga ttccgtgctg ttataacgat agttacctga
     6301 atgcgtaatc aggtaactgg caagcttttg cgtaaagcag cattcgccgc gcaggacgcc
     6361 ttgcaaccca ttgacaacac gttcttgatc ctccatggca taaaaaacgc cgttgatatg
     6421 aggccagttt tcaatgtcgc ggtacgggta atcttcaggc gtatttagca acaatatttt
     6481 gatattgttg tttttcctgc tcaaagtatc ctgccaataa tggataagct ttttatccgc
     6541 ttccatcata tccagaagaa taatagagcc tgaagatata tcgtccagag aacgttgaat
     6601 attatgtaat tttcctgtaa ttgccagcga ttgtttaagg tgctgcaaga gagctgtcgc
     6661 ctgcaaagaa ggtttagtga tcaacaataa tgtatgacca tgaatactat ggacttcatt
     6721 aaacatgatg aaaccccgct ttttttattg atcgcacacc tgacagctgc ctctaaaata
     6781 gaagcaccag aagtactgac agatgttgca ctgctgtgtg t
//
LOCUS       DAZFRG010000240         6803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFRG010000240 DAZFRG010000000
VERSION     DAZFRG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.241"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..783)
                     /gene="artM"
                     /locus_tag="ACHKUC_004094"
     CDS             complement(115..783)
                     /gene="artM"
                     /locus_tag="ACHKUC_004094"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO6620253.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(783..1499)
                     /gene="artQ"
                     /locus_tag="ACHKUC_004095"
     CDS             complement(783..1499)
                     /gene="artQ"
                     /locus_tag="ACHKUC_004095"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO6620254.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAVIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1506..2237)
                     /gene="artI"
                     /locus_tag="ACHKUC_004096"
     CDS             complement(1506..2237)
                     /gene="artI"
                     /locus_tag="ACHKUC_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO6620255.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2255..2983)
                     /gene="artP"
                     /locus_tag="ACHKUC_004097"
     CDS             complement(2255..2983)
                     /gene="artP"
                     /locus_tag="ACHKUC_004097"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO6620256.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3201..3716)
                     /gene="ybjP"
                     /locus_tag="ACHKUC_004098"
     CDS             complement(3201..3716)
                     /gene="ybjP"
                     /locus_tag="ACHKUC_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6620257.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3842..4165
                     /gene="ybjQ"
                     /locus_tag="ACHKUC_004099"
     CDS             3842..4165
                     /gene="ybjQ"
                     /locus_tag="ACHKUC_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO6620258.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4162..4992
                     /gene="amiD"
                     /locus_tag="ACHKUC_004100"
     CDS             4162..4992
                     /gene="amiD"
                     /locus_tag="ACHKUC_004100"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO6620259.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4989..6002)
                     /gene="ybjS"
                     /locus_tag="ACHKUC_004101"
     CDS             complement(4989..6002)
                     /gene="ybjS"
                     /locus_tag="ACHKUC_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6620260.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQGELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6101..>6803)
                     /locus_tag="ACHKUC_004102"
     CDS             complement(6101..>6803)
                     /locus_tag="ACHKUC_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO6620261.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLQALWRVV
                     NQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKRIARLAEQSTD"
BASE COUNT         1666 a         1779 c         1791 g         1567 t
ORIGIN      
        1 aaataaaaaa agacggacag attagtgggt tgcccgtctt cataataaga atttatgcac
       61 tatgtaggcc ggataaggcg tccacgccgc atccggcatc agcatgcagc tacattaatt
      121 ccgccgttcg aatgccagtg ctttacgctc gatcagacgc atcatcagcg tcagcaggcc
      181 gttgacgacc aggtaaataa tccccgccgc gccaaatacc attacatcgt aggtgcgtcc
      241 gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtaag ccagagaggt
      301 acttttgaac accagcacca cttcgttgga ataagaagag agcgagcgtt taaacgcgta
      361 cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac aggactgcca
      421 ttgaccttcc gggatcgcgc gaatcgcacc gtaaaacagc tgcgtggtgt acgccgcact
      481 gttcagcgac aacgcaatca gcgcacataa ccacggttct gacaacaaat gccacagtgc
      541 cggatactcc tgcaacgttg ggaactggcc cggtccgtaa taaatcagga agatctgcac
      601 cagcagcggc gtaccggtaa acagcgtgat atagccccgc accagccaca ccagcaccgg
      661 cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca caatcagcga
      721 ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc agttcgggta aatactcaaa
      781 cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt gagaatgtac
      841 tgactgagca gggtgatcac caggtaaatc accgccgcca caatgtacca ggtaaacggt
      901 tcctgggtac gggtagcgat gcttttggtt tgtagcatta aatcattcac actaatcaaa
      961 ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgagacc cggcagcgca
     1021 tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga cagccccagc
     1081 gcctgaccgg attcccactg acccaccggc accgctttta atgcaccgcg cagcgtttgc
     1141 gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa cgggctaacg
     1201 tcgaagttct caatgtccat ctgcaccggg atctgcacga acccaagatt gatagtgaag
     1261 ccatccgaaa gcgtcagcag cagctgcgag gagccaaaat agataaacag caccaccaga
     1321 atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc gacaggacgc
     1381 catttcgcag actcccatac cgcaaagaac atcgccagcg ccagcccgac aatcaatgca
     1441 caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa ttcattcatc
     1501 aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc tttcttcact
     1561 ttttccagcg cagtgttgag tttctgctgc agttcagtgt tgccctggcg tactgcgatg
     1621 ccgaggccag taccgaagta atctttatcg gtcactttat cgcccactgc cgccagtttc
     1681 gggttatctt tcagccattc tgttaccact gcggtgtcac caaatacgct gtcgatacga
     1741 ccgttttgca gatccaattt tgcgttctgg taactgtcat atggcacggt agtgatttcc
     1801 ggatgcttat ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc gacttttttg
     1861 cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag ggcagagttg
     1921 tcatagtacg gggtggtaaa cagcacctgc ttttcacgct ccggagtgat atccatgccc
     1981 gccatcactg cttctacgcg acggaatttc aggcttggga tcaggctgtc aaacgcctgg
     2041 ttagagaaag tgcaggttgc atcaatctct ttacacaatg cctgcgccag gtcgacatca
     2101 aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggatagga ggcttcggtg
     2161 gcaaaacgaa tggtttcggc tgctgtggcg gaaagactaa aacctgcaat taacgcggca
     2221 atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag tttttaaatg
     2281 cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata tgaccgtttt
     2341 ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg gtgacgatca
     2401 cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt gccgtaattt
     2461 cggggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc atcatcaacg
     2521 cacgggcaat agcaacacgc tgctgctgac ctccagaaag atgcagcggg taacgatcgc
     2581 tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc gcctgatctt
     2641 tactcaaccc cagtacacga cagggcgctt caatcaggtt ttgctgcacg gtcagatgcg
     2701 gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg cgaatcgctt
     2761 tgtcagaggg ggttttggtg aaatcgaaat ggttgcctgc aatgttgagc gtaccggagc
     2821 gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg ccgctggggc
     2881 caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac agcgcctgat
     2941 gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact cgtatactgg
     3001 cagtctgata gctattgagg tcgaagatag tacctttgac agaataatta tgcaatattt
     3061 ctgctttaaa agttaaaagc aaagcgcatt attcaataaa catagcacaa aataacgggg
     3121 gcggtggtcg gcgagcataa atgtcggcat tcctcacgaa atgccggaca atttacgggg
     3181 tttattggtt gatcaagacg ttagcgattc tcgatggatt gacggagcgt acccgccgtg
     3241 gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga cccaacactg gccttcctga
     3301 atcattaaca cttcatcctg ccagccctgg tcaccctgct tgagatccac gcgcaatgga
     3361 atgttacggg catcacgatt agggatagta gatgcactgg caacgtgggc gctgtctggc
     3421 aaggtggttc gactggagaa cggatcgctg cttaacaatt cacgatgact attatcgcgg
     3481 ctggcatcgc taagtagggt tgccagtttg tcactcaaat aggggcgcag agcggtaatg
     3541 tcattgctgc gatgttgaat gtggtagtca tagaactgtt gagccacgct atcaggcccc
     3601 ccctggacac aggaaccggt gcgggggccg ttatctttat aagctggcgt gacggtggta
     3661 caggcactga gtagcagtgc gcaagggata agcatggtca atttgctata gcgcatgatg
     3721 atttccttat aagcgaacac cttgaaagcg ttctacgata ataatgatat cctttcaata
     3781 atagcgtatc agtccgataa tgctttgaag atcgaaggct tagcaaacaa ggagatcgat
     3841 catgcaattt tctacaaccc caactctgga aggccagacc atcgttgaat attgcggtgt
     3901 ggtgactggc gaagcgattt taggcgcgaa tattttccgt gatttctttg ccggtatccg
     3961 cgatatcgtt ggcggacgct ccggtgccta cgaaaaagaa ctgcgtaaag cgcgggagat
     4021 tgcctttgag gagctcggct cccaggcgcg agcgctgggg gctgatgccg tcgtcggtat
     4081 tgatatcgac tacgaaacgg tcgggcaaaa cggcagtatg ctgatggtta gcgtcagcgg
     4141 tacggcggtg aaaacgcgtc gatgagaaga gtcttctggc tggtcgctgc cgctctgtta
     4201 ctggcagggt gcacaggcga aaaaggcatt gtcgagaaag agggatatca gcttgatacc
     4261 cgacgccagg cgcaggcggc gtatccgcgc attaaagtgc tggtgatcca ctacaccgca
     4321 gatgattttg atagctcgct ggcgacactg accgataagc aggtcagctc gcattatctg
     4381 gtccctgcgg tgccaccgcg atacaacggt aaaccgcgca tctggcaact ggtgccggaa
     4441 caagaactgg cctggcatgc ggggattagc gcctggcgcg gggcaacgcg ccttaacgac
     4501 acctctattg gtattgagct ggaaaatcgt ggctggcaga aatcggcggg ggtgaaatat
     4561 tttgccccgt ttgaaccggc acagattcag gcgcttattc cgctggcgaa agatattatt
     4621 gcccgttatc acatcaagcc ggaaaacgta gtagcacatg cggatattgc gccgcagcgc
     4681 aaagacgatc cggggccatt atttccctgg cagcaactgg cacagcaggg gattggtgcc
     4741 tggccggatg cgcagcgggt gaacttttac cttgccgggc gcgcgccaca cacaccggta
     4801 gagaccgcgt cattgctgga gcttttggcg cgttatggtt atgacgtaaa gcctgatatg
     4861 actccgcgcg agcagcggcg cgtgattatg gcgttccaga tgcatttccg cccgacgtta
     4921 tataacggcg aagcggatgc agaaactcag gcgattgccg aagcgttgct ggagaaatac
     4981 gggcaggatt agcgcggcag ttttccgtgg tcgcgtagcc aggcggcagt tttctcgata
     5041 ccttcatcca gggtgaggac tggctgataa cctaactctc cctgcgcacg cgtaatatcc
     5101 agcgtaaagt caaaattcaa cttggagacg ccgtagtggg tcagcggcgg ctctttagct
     5161 gacttgcgac ctaaacgctc catgctgcgg gcgatcatat ccagcatcgg gtaaggaacc
     5221 gaacgaatac gacagtcaat attcaactcg tcgatcagct tctgcacgat gctgcgcagt
     5281 gtgcgatgct cgccgttggt gatgttgtac acacgcccgg aaggtagctt atcgcacgct
     5341 tcctggcttg ccagccacat ggcgtgcacg gcattttcat agtaggtcat atcgaccagc
     5401 gcactgccgc catgcggtaa cagaatactg ccgtagtgat gcatcatatg cgccagacgg
     5461 gggataaaga ctttatcgtg cggtccgaac agactttgtg ggcgcagaat agtaaagcgc
     5521 gtttgtggat tcgcctgcga aagcatattg atcacttctt cactggctgc tttgctgcgg
     5581 gcaaactcgt tggcgaagcg gtgagggcga aaatcttctt taatatcgcg atggtggtga
     5641 taatcgaagt acagggaggg agaagagata tgaataaagt tacgcacacc ccaggcgaca
     5701 gcccattcac ccaggcggcg agtggcgcga acgttagcca gatcgaaagc ctgttgtgtc
     5761 ccccagggcg aggtaaagct ggagcagtgc cacagcgtat caatgcccgc gagcatcact
     5821 ttagcttgtg atgagaccag ctcggtcaga tccgccggaa caaactctgc gcccattttt
     5881 tccagcaatt tacccattgc ctcattgcga ccggtcgctc gcacgctgat gcctttctgg
     5941 cataaaaact ctaccgcgtt tcgacctaag ccgctggtgg cgccggtaac cagtaccttc
     6001 atatcaatcc actgttgttg agaaaatgac gtgcgcattc ttccgtgatt tcccccttga
     6061 tgcaatggga aacatgaaag aataacgcag gttttgtcga ttaatctgtg ctttgttctg
     6121 ccagtctggc gattcgtttt gccattccgc gaaaaataaa cagatgcgcg gggatcatca
     6181 ataaccagta aaacagcccc ggcataccgt gcggatgcca gaaagcacgg acatcaatag
     6241 tacgatagtc gcctttatct tccagggtaa aacacagtcg tcccagcccc ggcgctttca
     6301 tgccaaataa caacgtaagt tgtttttccg gttcaacgac aatcactttc cagctatcca
     6361 ccgcatcgcc ggtctgtaaa tattcgcgct ccgggcggcc tttcgccagc ttatgaccga
     6421 tcgcaaggtc catcaacgcc cgtgtctgcc acaaaatatt gccaaagaaa taacgctctt
     6481 taccgccgat ttgattgacc acccgccata gcgcctgaag gctggcagac gttttaacgg
     6541 taaaccccgc ctgtttggca aaataaccgt attctggtcg ccagcgagca aaagcctgcg
     6601 cgtcatatcc ccagtcgctg gagttgacca gtttttcttc ctctttcaac gtgctacgta
     6661 ccgcgtcatc gaaagcgatc agccgttgtg ggatgagcgc acgtagcgcg gtgtcatccg
     6721 ccagcagatc gtgtttcagc ccctgaatca acgccctggc ggtggtgggc ggtactgaag
     6781 taatcacatt aagaaaccac acc
//
LOCUS       DAZFRG010000241         6787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFRG010000241 DAZFRG010000000
VERSION     DAZFRG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.242"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            184..1398
                     /locus_tag="ACHKUC_004103"
     CDS             184..1398
                     /locus_tag="ACHKUC_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620262.1"
                     /translation="MPVRLNTIPDAEPYPAPPAHIKWLSALLLMLSVGVLLTSLFASD
                     ELAKNGPHFWGLACGLPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILHETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFVQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1455..4783
                     /locus_tag="ACHKUC_004104"
                     /pseudo
     CDS             1455..4783
                     /locus_tag="ACHKUC_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
     gene            4776..6386
                     /gene="tssA"
                     /locus_tag="ACHKUC_004105"
     CDS             4776..6386
                     /gene="tssA"
                     /locus_tag="ACHKUC_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO6620263.1"
                     /translation="MSELLQKLTRSCFANRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSSVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMVLLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMDALLGTLVAINPVRAAVLCDTQHKE"
     gene            6392..>6787
                     /locus_tag="ACHKUC_004106"
     CDS             6392..>6787
                     /locus_tag="ACHKUC_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO6620264.1"
                     /translation="MGRGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGN"
BASE COUNT         1464 a         1909 c         2064 g         1350 t
ORIGIN      
        1 aataacaata ctgaaagaat ttaagaattc acatggtttt aaattaagct ttaaatgata
       61 tttctgtata aaaagcaaga tgtattttaa ttagtgcgta cctaagttgg tagtgaatta
      121 cttacatagt ttaaacatcc tgatattcat tacatttata tttcagaata atggattatt
      181 acaatgcctg tccgacttaa cactattcct gatgctgaac cctatcccgc accgccagcc
      241 cacattaaat ggttgtcagc cttattactg atgctgtctg tgggcgtgct cctgacttca
      301 ttattcgcca gcgatgaact ggcaaaaaac ggcccgcatt tctgggggct ggcctgcggt
      361 ttgcctgcct ttatctggtc gctggttgct tcagttcgct ggctgttttt tatcacgcag
      421 tacattcggg ctgatgcctg gaataagcgc cgggaagagg tgattttgca tgaaacgcga
      481 cggggacgac gggcgttgca gatacttagt ttttccgtac aaaccgcgct gaatggtgat
      541 tccgttgcag aaactacaac agcatttctg gcgcgtcagc aggtgttgaa tacttacgct
      601 gatttgcgcg gagaagatac cgtgcgtcgc agtgtcattc ctgtattaac caataaaccg
      661 gttaccgggc gtttatccga aattatttcc cgtcttttta ttgatatcag accgcaattg
      721 tttctgctgc cccctgattt tcatatcaac gtactactgg aaattgatgc gccgctttca
      781 ggggcctccg tgagaactat ctggcaggac gaatggcaaa aagccggact cccggaagcc
      841 cgtctgtttt ctgccccgga accggggctg gccgccgtgg acgactggct ggacaacttt
      901 gttcaggaaa aggccgtact gctggtgatt tccgtacgac ttgaaccgaa gaacccggaa
      961 cggacggcag aatccgccac cgcgctgttg ctggcaaacc gactgacgca aaccgcgctg
     1021 acgccgctgg ccctgctgca tcgtcctgaa cgtatcacag atacagagat gatggcgtca
     1081 ggcattgcac aggcgctgga ctggatgccg gtgcagccag atgccatatc cggtatgtgg
     1141 actgccgaac tggacaggga acagcgtgcc gcgttgttaa gcctgaacca gccgtttgct
     1201 caggaagccc tgatgtacga gctggatgcc tttctggggc gctccggacc ggctgcgccg
     1261 tggctgtccg tcgctgtggc aacgctggcg gcaatacaat cacaacaccc ccaactgacg
     1321 ctcagcggcg ttcagggcgg gcattattca tgggcgaccg tggtgtcgcc attcgtttcc
     1381 cctcaggagg catcatagtg gttccgggat taaaccgtct ccggccccgt tcggtttcag
     1441 ggcctgccat cgtcatgacc gtggctgttg tggttgcgct ttgcacgttt attcaccgta
     1501 aaccggcatt gctgggcgtt gaaccgggta gcccacagca aatgtactgg atggctggcg
     1561 tgattgccgc aggcgtggtc tttctgatta tcatgggggt ttatcatctg ctgaaccgcc
     1621 acgctgccgt gcagaacttt gaactccgca gacagcttgc cgccggagat gactatcgtg
     1681 cggtgacgac aaagaaagcc gacagccgtc atccggcaat ggtggaactc gccgccttcc
     1741 tgcgcgaccg ctacggccca ttctggcggc gtaaagtccg cctgctgctg gtgaccggcg
     1801 agcctgaaga ggcagaggcc atcgcgccgg ggctgaccgg gcaacactgg ctggagggcg
     1861 accacacggt gctgatatac ggcggcaggc catcagcgga gcctgatgtc gcgctactga
     1921 ccgccttaaa aaaactgcgc cgcagccgtc cgctggacgg catcatctgg ccgctgacag
     1981 aagaacagag ccgccagacc gcacaactcg acaaaggctg gcgcggactg ataaacggcg
     2041 gtaagcgact cggttttcag gctccactct atttgtggca ggtctgtgac gacggtgatt
     2101 atcagaccgg acgccccctg caaaacgtcg gctgcctgct gccggaacgc tgtaccccgg
     2161 aacaactggc tgtaatgctg gaagcacaaa ccctgccgct gacggaacag ggcatgtcgc
     2221 aactgctgac caataaccgc catgatttcc tgctgcgtct ggcccatacc cttgcagagc
     2281 ggggtatcgc tcactggcaa agcgttctga aaccgctgct ggcaggcggc gcattttctt
     2341 ccctgcgcct gcgtggcctg atgttcagcc cgccgcttgc cgccgtgccg gaggccgcac
     2401 ctcatgcgtg gctgccgtca ccggtctggg cgggcgtgac gggtgataac gcgcgcgggc
     2461 gcacggtggg ttttccgtgg ctgcgtaccg cactgatgtc cgctgtctgc gtgctggtga
     2521 tatgggggcc ggaatgacga cctcgttctt cgccaaccgc gcgcttgttc aggaaaccag
     2581 catccagacg gcgcgtgcgc tcgatacccg cctgccgctg gcagaacaac tggtggcact
     2641 gcataccctg cagggcgaac tggagcgcct gcaataccgt atccgtgagg gtgcgccgtg
     2701 gtatcagcgt tttggccttg aacgtaacca gcaactgctc gctgccgcct ttcccggcta
     2761 tgcgcaggcg gcaaaccggc tggtgcgcga cgtggccgtt gaccatctgc aacagcaact
     2821 gaacgccttt gtcgcgctgc cgcccaacag tcctcagcgt accgccaccg gtgaacaacg
     2881 ctataagcag cttaaggcat tgctgatgac ttcccgcccg gaaaaggccg acgctgcctt
     2941 tttcagtacc acgctgatgg cagacggcct gcgctacgag aatatcccgg aaggcgtgcg
     3001 gcagagcgtg ttgccgtcgc tgctgacctt ctggatggcg aacctgccgg aacacccgca
     3061 gtggaaaacc gcgccgccgc cggaactgac cggcgcggta cgtaaaatcc tgctgcgcca
     3121 gattggtgtg cgtaatgccg aaaacaccct ctaccagaac gtgctgcaac aggtgtcccg
     3181 caactacgcc gatatgacgc tggcggacat gaccggggat accctcaccg aatctctttt
     3241 cagtacagaa cagacggtgc cggggatgtt cacccgtcag gcgtgggaag gacaggtcag
     3301 ggaagccatc gagcaggtgg tgacggcgcg gcgcgaggaa atcgactggg tactcagcga
     3361 ccgccagcag gatacctctg cggatatctc gccggatacg ctgcgtaacc gtctcacctc
     3421 tcgctacttt accgacttcg ccggaagctg gctggcgttt ctcaacagca ttcgctggaa
     3481 aaaggaagac tcactctccg gcattctcga ccagctgaca ctgatggccg atgcccgtca
     3541 gtcgccgctg attgcgctga cggacaccct cgcgtggcag gcggcggcag gcagggaaaa
     3601 ccgtggtctg tcagactcgc tggcgaaatc ggcacaggaa ctgtttaacg gcaaggagaa
     3661 aacgccgcag caatcccgtg aaggtgacga cgtgcctgtc gggccgctgg ataaaacctt
     3721 cgcgccgctg ctgcggttgc tgggcgataa ggccggaggc ggcgacagcc agctgagtct
     3781 acagacctac ctcacccgcg tcacccgcgt gcgtctcaaa ctgcaacagg tgaccaacgc
     3841 ccccgacccg caggagatga cccaacagct ggcgcagacg gtcttacagg gtaaaaccgt
     3901 tgacctcaca gacacccgcg actacggacg gttaatcgcc gccagtctgg gcgaggaatg
     3961 gagtggcttc ggtcaggcgc tgttcgttcg cccggtagag cagtcgtggc ggcaggtgct
     4021 gacgcctgcg gcggacagcc tgaaccgcca gtggcagcgg gcgattgtca gactctggga
     4081 tcaggacttc gccggtcgct acccgttcaa agcctcacaa aacgatgcat ccctccccct
     4141 gctggcgcag tacctgcgcg atgacgggcg catcaaccag tttattgcca acaacctttc
     4201 cggcgtactg aaacgtgaag gccgctactg ggtggctgac gccatgaaca cgcaggggct
     4261 gacggttaac ccggacttta tccgcgccct gaaccgcctg cgcgacgtgg ccgataccgc
     4321 ctttgccagc ggcgatgccg gaatacattt tgaactgcgg gcaaaaccgg cccgtgacgt
     4381 gatgaaaaca catctggtga ttgacgggca ggaactggaa tatttcaacc agaaagaacg
     4441 ctggcagcgt tttaactggc cggacgaaca gtggcaaccc ggcgtatcgc tgagctggac
     4501 cagcacacag gcgatggaac gcatactggc ggattaccgg ggaagctgga gccttattcg
     4561 cctgctggaa caggcgcagg tgacgccggt ggacagcagc acctttaagg tggtgtggaa
     4621 agcgcaggac ggcctgccgc tgaattacct gctgcgggtt gaacagggca aagggccgct
     4681 ggcgctgctg gaactgaaaa acttccgctt gccggggcag gtatttctga ccggcaggag
     4741 tatgacggat gcggaagaat atggggaaga cgccgatgag tgaactgttg caaaagctga
     4801 cccgtagctg ttttgccaac cgggacgcac tggatgtggc gcgaacacag gcggcactgt
     4861 ggcagacgtg gctgctgccg gtgacggcag atacgccggt cggggaagac cccggctacc
     4921 acgacgattt cctgcgtatc cgcgacgaaa tgaacaaact ttccggcgcg gataccggcc
     4981 tcatctgcca gcttgccgaa tccctgctgc tgacgcaggc aaaagacgtg cgcatcgcca
     5041 cttactacat ctgggcgcga ctgcaccgcg acggtgagcg cggactggct gaggggctgg
     5101 cgctgttagc cgggctggtg gaacgcttcg gtacgcaact gctgccctcg cgtccggcaa
     5161 gccgaaaaat ggcgctggag tggctggcag gtgaaaaaat gctcgacagc cttgcccgct
     5221 acccggaagt ggcaaaagag gattttgcga acattgttgc cgctcttaat caactgacgg
     5281 tttccttcac tgcatggccc gaagaccagc attccccctc cctgatgccg ctgattaacg
     5341 cgctggaatc acgtctggcg cagtcgggtg ggatgaacgc ggtggtgcca caaaacagca
     5401 gcagcgttcc tgcaccgtct tccccggtgg atgcgccaca ggtacagacc atcacttccg
     5461 ggcgcgacct gctcgaccag gcgaaagtgc tggcccgtta tctgaacgaa cagccgcagg
     5521 gctggctttc agcacaccgg ctgatgaaaa ccctgcgctg ggacactgtg catgaactgc
     5581 cgccggatgt ggatggcaaa acccggctgg ccccgccgcg caccgaatca cgcaaccagc
     5641 ttaagcggct gtatgcccag cagaactgga cggaactgct ggagcaggcc gacctgatgt
     5701 tcagcaccgg tgtcagccat ttctggctgg atatccagtg gtatctgcat caggcgctgg
     5761 cgaaggcggg tgctccgtgg gacagatgga ctgcggttat ccgtcaggat ctggcgctgc
     5821 tgctggaacg cctgccaggg ctggaaaatc tggcatggaa cgacggcaca ccgttcgccg
     5881 acgaggtgac gcgcaactgg atagcgcagc aggtcatgat gcgggaagac ggcgcatggc
     5941 ttgcggggaa agctgctgtg ccgacagacg acgccacaaa cgacgtactg gcgctggagc
     6001 cggaagcgct ggagatggca gacagtcagg gcgtggaagc ggcactgggc tggatacaga
     6061 cccgtccagg catcaccacg gcacgacagc gcctgttatt gcggctgctg atggcacggg
     6121 tggcggaaca gtacggtaaa aacgagatgg tgctcctgct gctggaagaa ctggatactg
     6181 ccgcacaggg aataacgctg acgcagtggg agccggaact gctgtttgag gtaaaagccc
     6241 gacaactgaa actgctgcgt ctgcgggcgc accgctatgc ggataaagcc ctgctgaacc
     6301 gaaaaatgga tgccctgctg ggtacactgg tggcaatcaa tccggtgcgg gcggcggtac
     6361 tgtgtgatac gcagcataag gagtgatgac gatgggaaga ggttattttc tggtcagggg
     6421 cgataagacc acctgcggcg gcaaaattat tgaaggggcg gacgaccata cgatcatggg
     6481 tatcccgcag gcgcgggata tggaccgggt cacctgcggc agatatccgg gaatgtttat
     6541 tattgtgggc ggcgttcctg aaacagatat tcacggcagg ctgatggcgg ggacgctgga
     6601 cagtcagagc agttgcccgt gtaaagcacg gtttattgcg tcaatgatgg atgatacgta
     6661 tgagacggat gacggggaaa gtgaagcgga acaacatgcg cagtcggcga aaaaggatct
     6721 gacttctggt tctgattcat cctctgatga tgtaaaactg gattacagaa ttaaattatc
     6781 aggtaat
//
LOCUS       DAZFRG010000242         6780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFRG010000242 DAZFRG010000000
VERSION     DAZFRG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.243"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..2316)
                     /gene="maeB"
                     /locus_tag="ACHKUC_004107"
     CDS             complement(37..2316)
                     /gene="maeB"
                     /locus_tag="ACHKUC_004107"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO6620265.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2605..3555
                     /gene="tal"
                     /locus_tag="ACHKUC_004108"
     CDS             2605..3555
                     /gene="tal"
                     /locus_tag="ACHKUC_004108"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6620266.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3575..5578
                     /gene="tkt"
                     /locus_tag="ACHKUC_004109"
     CDS             3575..5578
                     /gene="tkt"
                     /locus_tag="ACHKUC_004109"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6620267.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5655..6653)
                     /locus_tag="ACHKUC_004110"
     CDS             complement(5655..6653)
                     /locus_tag="ACHKUC_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO6620268.1"
                     /translation="MSLVWAAPAQRAFSDWQVTCNNQNFCVARNTGDHNGLVMTLSRS
                     AGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDKWRISPWLLVTDDTAT
                     ITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVGSETAWIKKGDEPPLS
                     VPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSLDPLRREVNVTALTDD
                     KALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQETNELELMNATFDEK
                     SRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNWHGPDAWPTLWITR"
     gene            complement(6592..>6780)
                     /locus_tag="ACHKUC_004111"
     CDS             complement(6592..>6780)
                     /locus_tag="ACHKUC_004111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620269.1"
                     /translation="TNPIEPRYLRNEDTTCAVCLNFWGRTVHAISHFPSLFFRFVADV
                     FGVGGTSATGVFRLAGHL"
BASE COUNT         1632 a         1831 c         1760 g         1557 t
ORIGIN      
        1 tgaatattca gggtaagcgt gagagttaaa aaagaattac agcggttggg tttgcgcttc
       61 aaccacggcc agcgccacca tgttgacgat acgacgtacc gatgcgatcg gcgttaacac
      121 gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca gtcacacctt ccgagctgga
      181 aacgcgcagt aagttgtaac taatgcgggc agcttccatg ttcggcatca ccagaatatt
      241 agcggaacct ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc
      301 tgcatcgccg tgcatttcac catcaatcat cagttctggc gcacgttccc tgaccagttc
      361 cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga
      421 caacagagcc acacgcggct caataccaaa acgacgaaca gtttctgccg ccatcaaggt
      481 gatctccgcc agctcttctg catcaggctc atcattgacg taggtatcgg caataaaggt
      541 attaccactc ggcaacagca gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata
      601 accaaagaca tttttcacca cgctaaagtg ttcatgataa tcgcccaccg taccgcaaat
      661 cattgcatcg gcttcgccac gctgaaccat gatcgcgccg atcactgtcg ggttactgat
      721 cagcgcacgc tgcgcctgtt cctgagtgac accgcgacgc ttcatgatct ggaagtattc
      781 ggtccagtac tctttaaagc gcggatcgga ttcgttattg acgatctcaa aatcaacgcc
      841 cgccttgatc tgcaagccca gtttctgaat gcgcatttcg atcacgttcg gacgaccaat
      901 aaggatcggt ttcgccagtc ccagcgttac cagttcctga gtggcatgca gaacgcgcgc
      961 ctcttcccct tccggcagaa ctacgcgctt cggcgctttg cgagcctggg agaaaatcgg
     1021 cttcataaac aggttagttt tgtaaacgaa ctctgtcagc ttgtcgatgt agacgtcgaa
     1081 atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc gctttcgcca ccgcaggagc
     1141 gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg atgtattccg gaccaaagct
     1201 cagatcctga tcgccatacg ctgaagccac cacttcactc tgttccgcat gggccagttc
     1261 tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg atggcggttg cgccaacgtc
     1321 cagcgcgcca cggaagataa acgggaagca aaggacgttg ttcacctggt tcgggtagtc
     1381 ggaacggccg gtacagatga tggcatccgg acgcacttct ttcgccagcg gcggcagaat
     1441 ttccggttcc gggttcgcca gcgccaggat cattggcgca cgggccattt tcttcaccat
     1501 ttcctgggtc agcactttcg ggccggaaca gccgaggaaa atatccgcgc cttcaatcac
     1561 atcatcgagg gtacgtttac cgtcatccac caccgcgtat gcggctttgg tttccgccat
     1621 gtttggctca cggccctgat agataacgcc ttttgaatcg caaaccacga tgttatgttt
     1681 ttgcagaccc agcgctacca gcaggttcat acaggcgatt gctgcggcac ccgcgccaga
     1741 aaccaccatc cgcacgtcgg agatgttttt ctccaccacg cgcaagccgt tgaggatggc
     1801 ggcagtgctg ataattgccg tgccgtgctg atcgtcgtgg aataccggaa tattcatccg
     1861 ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt cgaggttgat
     1921 gccgccgaag gtcggttcga gcgcagcgac gacttcaata aatttgtccg ggtcgagttc
     1981 atcaacttca atgtcaaata catcaatccc ggcgaatttc ttaaacagaa cgcccttgcc
     2041 ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc
     2101 gttagagatc accgctacca ggttaccgcg cgcggtatat ttgtaggctt ttaacgggtc
     2161 tttttcgatt tcaagacaag gtgcggcaac gcctggtgag taggccagcg ccagatcgcg
     2221 ctgcgttgcc agaggcttgg ttggagaaac ctggattttc cctggaactg gaaattcatg
     2281 gaaatcaagt gcactttgtt ttaactggtc atccatttgg ttgttccttt cacgtaacgt
     2341 tcacaaataa agtgtgtggg caacagcccc tgtccacaac gtggcgcaca ttattaccct
     2401 gccggagtct acagactttg agcaagtcca aactctcacc attaatataa tgttttggta
     2461 ataatcctat aacactgatg ttacctgctt aatccagcaa taccatgcct gtctgctatg
     2521 cttttttgat gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact catctaacac
     2581 tttacttttc aaggagtatt tcctatgaac gagttagacg gcatcaaaca gttcaccact
     2641 gtcgtggcag acagcggcga tattgagtcc attcgccatt atcatcccca ggatgccacc
     2701 accaatcctt cgctgttact caaggctgcc ggattatcac aatatgagca tttaatagac
     2761 gatgctatcg cctggggtaa aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg
     2821 tgtgacaaac tggcggtcaa tttcggtgct gaaattctca aaatcgtacc cggtcgcgtg
     2881 tcaacagaag ttgatgcacg cctctctttt gataaagaaa agagtattga gaaggcgcgc
     2941 catctggtgg acttgtatca gcaacaaggc gttgagaaat cacgcattct gatcaagctg
     3001 gcttcgacct gggaaggaat tcgcgcggca gaagagctgg aaaaagaagg tattaactgc
     3061 aacctgacgc tgctgttttc ttttgcacag gcgcgggcct gtgcagaagc aggcgttttt
     3121 ctgatttcgc cgtttgtcgg gcgtatttat gactggtatc aggcacgcaa gccgatggac
     3181 ccgtatgtgg tggaagaaga tccaggcgtt aaatcagtac gcaatatcta cgactactat
     3241 aagcaacacc actatgaaac cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc
     3301 ctcgccttaa ccggctgcga ccgactgact atcgcaccga atttgctgaa ggagctacag
     3361 gaaaaagttt cgccagtggt acgtaaatta atcccacctt ctcagacgtt cccacgccca
     3421 gctcccatga gcgaagcgga gttccgttgg gagcacaatc aggatgcgat ggcggtagaa
     3481 aaactgtctg aaggcattcg tctgttcgcc gttgatcaac gcaaactgga agatcttctt
     3541 gccgccaaac tataaaccag ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc
     3601 gattcgcgca ctcagtatgg atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc
     3661 gatgggcatg gctgatattg ccgaagtgct gtggaacgat tttcttaaac ataaccccac
     3721 cgacccaacc tggtatgacc gtgaccgctt tattctttcc aacggtcacg cgtcgatgct
     3781 gctctacagt ttgctgcatc tgaccggtta cgacctgcca ctggaagaac tgaagaactt
     3841 ccgtcagttg cattcgaaaa cccctggtca cccggaaatc ggctatacgc caggtgttga
     3901 aaccaccacc ggtcctcttg gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga
     3961 gcgtacgctg gcggcgcagt ttaatcagcc ggatcatgaa attgtcgatc acttcaccta
     4021 tgtgtttatg ggcgacggct gcctgatgga aggtatctct cacgaagtct gttcgctggc
     4081 gggcacgctg gggctgggca agctgattgg tttttacgat cacaacggta tttccatcga
     4141 cggtgaaacc gaaggctggt ttaccgacga taccgcaaaa cgatttgaag cctatcactg
     4201 gcatgtgatc catgaaatcg acggtcacga tccacaggcg gtgaaggaag cgatccttga
     4261 agcgcaaagc gtgaaagata agccgtcgct gattatctgc cgtacggtga ttggctttgg
     4321 ttcgccgaat aaagcaggta aggaagaggc gcacggcgca ccactggggg aagaagaagt
     4381 ggcgctggca cggcaaaaac tgggctggca ccatccgcca tttgagatcc ctaaagagat
     4441 ttatcacgcc tgggatgccc gcgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa
     4501 gtttgccgcc tataaaaagg ctcatccgca actggcagaa gagtttaccc gtcggatgag
     4561 cggcggtttg ccgaaggact gggagaaaac gactcagaaa tatatcaatg agttgcaggc
     4621 gaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat acgcttaacg cttacgggcc
     4681 gatgctgcct gagttgctcg gcggttcggc ggatctggct cccagcaacc tgaccatctg
     4741 gaaaggttct gtttcgctga aggaagatcc ggcgggcaac tacattcact acggggtgcg
     4801 tgaatttggt atgaccgcta tcgccaacgg catcgcgcac cacggcggct ttgtgccgta
     4861 taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac gccgcgcgga tggcggcact
     4921 gatgaaagcg cggcagatta tggtttatac ccacgactca attggtctgg gcgaagatgg
     4981 tccgacgcac caggctgttg agcaactggc tagtctgcgc ttaacgccaa atttcagcac
     5041 ctggcgaccg tgcgatcagg tggaagcggc ggttggctgg aagctggcgg ttgagcgcca
     5101 caacggaccg acagcactga tcctctccag gcagaatctg gcacaggtag aacgaacacc
     5161 ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc
     5221 agatattatt ctgattgcca ccggttcaga gatggaaatc accctgcaag cggcagagaa
     5281 attagcggga gaaggtcgca atgttcgcgt agtttccctg ccctcgaccg atattttcga
     5341 tgcccaggat gagaaatatc gggagtcggt actgccttca aatgtttctg cccgcgtggc
     5401 agtagaagcg ggtattgccg attactggta caaatatgtt ggcctgaaag gggcaattgt
     5461 cgggatgaca ggttatgggg aatcagctcc ggcggataag ctgttcccgt tctttggctt
     5521 taccgccgag aatattgtgg caaaagcgca taaggtactg ggagtgaaag gtgcctgatg
     5581 gtgatcgccg gatgctgatt gtcggatgtg acgctggtgc gccttatccg gtctacatgt
     5641 ccccgccatt ttgtttaacg ggtgatccac aacgtgggcc aggcgtctgg cccatgccag
     5701 ttatcgcagg tgggttctgc ggcgtaacgc accaggcgaa aacgctgacc gtcaaagcgc
     5761 cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt ttgctaaggt caccagttca
     5821 cgcgattttt catcgaatgt tgcgttcatc agttccagtt cattggtctc ctgaccgttg
     5881 ttgaacggca aacgcaaccg taccgggcgc gaggccagcg gctttttacg cgacacaatc
     5941 catgccaaat caatggtgtt ataggcccca gcctcacagc taatcatcat cagcgcttta
     6001 tcatcggtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga
     6061 ccattcatcc gccagtttcc ataatccagc aaatcgttac gttcttcgag tgagagtggc
     6121 gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgctggcgg tacgctgagc
     6181 ggcggttcgt cccctttctt tatccacgcg gtctcactgc caacgcgctt ttgctgggca
     6241 tcaataaaca acaacgccgc ttttaagcca ctgagagaaa tagtctgatt gccgtcgcgt
     6301 aaggtgatcg ccctcccttc ctgaatcatc tgcaaaaacg cggtgatggt tgccgtatca
     6361 tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaacgc caacggctca
     6421 ccatctaaca gcagccgtgg cgctatctcc ccttctgacg catccggcga cttcaacccg
     6481 ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccgg cgctgcggct cagggtcatc
     6541 accagtccat tatgatcgcc cgtattgcgc gctacacaga aattttgatt attacaggtg
     6601 acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga catcggcaac
     6661 aaagcgaaaa aaaagagaag gaaaatgcga tatcgcatgg acggtacgac cccagaagtt
     6721 caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcggatttgt
//
LOCUS       DAZFRG010000243         6680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFRG010000243 DAZFRG010000000
VERSION     DAZFRG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.244"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..1832
                     /gene="ybjD"
                     /locus_tag="ACHKUC_004112"
     CDS             174..1832
                     /gene="ybjD"
                     /locus_tag="ACHKUC_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO6620270.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1829..2785)
                     /gene="ybjX"
                     /locus_tag="ACHKUC_004113"
     CDS             complement(1829..2785)
                     /gene="ybjX"
                     /locus_tag="ACHKUC_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO6620271.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            2936..4051
                     /gene="macA"
                     /locus_tag="ACHKUC_004114"
     CDS             2936..4051
                     /gene="macA"
                     /locus_tag="ACHKUC_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO6620272.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4048..5994
                     /gene="macB"
                     /locus_tag="ACHKUC_004115"
     CDS             4048..5994
                     /gene="macB"
                     /locus_tag="ACHKUC_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO6620273.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNIIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6067..6291)
                     /gene="cspD"
                     /locus_tag="ACHKUC_004116"
     CDS             complement(6067..6291)
                     /gene="cspD"
                     /locus_tag="ACHKUC_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO6620274.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6441..6515
                     /gene="yljB"
                     /locus_tag="ACHKUC_004117"
     CDS             6441..6515
                     /gene="yljB"
                     /locus_tag="ACHKUC_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO6620275.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6614..>6680
                     /gene="clpS"
                     /locus_tag="ACHKUC_004118"
     CDS             6614..>6680
                     /gene="clpS"
                     /locus_tag="ACHKUC_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308994.1"
                     /note="involved in the modulation of the specificity of
                     the ClpAP-mediated ATP-dependent protein degradation;
                     binds to the N-terminal domain of the chaperone ClpA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HFO6620276.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDAL"
BASE COUNT         1581 a         1594 c         1861 g         1644 t
ORIGIN      
        1 attatttgat tttgttgttt ttgcagactt atcagcaaga gggagtataa cgcgattatt
       61 cgctcatttt tcagacattt gccatgctta aatgtgatgt catcacgtat tagcatggcc
      121 tttcccgtta tactgccagc gtaaaggata agtcacatat ttctggaggg gatatgattc
      181 ttgagcgcgt tgaaattgtg ggttttcgcg gtatcaaccg tttgtcgttg atgctggaac
      241 aaaacaacgt cctgattggg gagaacgcgt ggggtaaatc cagcttgctg gacgccttaa
      301 ccctgctgct atcgccagaa tcagatctct accattttga gcgcgacgat ttctggttcc
      361 cgccgggaga tatcaacggg cgagaacatc atctgcatat tattttgacc ttccgcgaat
      421 cgctgccagg ccgacatcgg gttcgccgct atcggccgct ggaagcgtgc tggacgccat
      481 gcaccgatgg ttatcaccgt attttttatc gtctggaagg ggagagtgcg gaagacggca
      541 gcgtgatgac actccgcagt tttctcgata aagacggaca tccgattgat gttgaggata
      601 ttaacgatca ggctcgtcat ctggtgcgtt taatgccggt gctgcgcttg cgtgatgccc
      661 gttttatgcg ccgtattcgt aacggcacgg tgccaaatgt ccccaatgtg gaagtcaccg
      721 cgcgccagct cgatttcctc gcccgtgaat tatcctcaca tccgcaaaat ctctctgatg
      781 ggcagattcg tcagggactt tccgcaatgg tacagctgct tgagcattat ttctctgagc
      841 agggggccgg acaggcgcga tatcgtttaa tgcggcggcg agccagcaat gagcaacgaa
      901 gctggcgcta tctggatatc atcaaccgga tgattgaccg acctggaggg cgctcgtatc
      961 gggttatttt gctcggcctg tttgctactt tgttgcaggc aaaaggcaca ttgcgactgg
     1021 ataaagacgc ccgtccattg ttgctgatcg aagatccaga aacccgttta caccccatta
     1081 tgctttcagt tgcctggcat ctgttgaatc ttctgccatt gcagcgcatt gccaccacca
     1141 actctggcga gctgctttcg ttaacgccgg tagagcatgt ttgccgactg gtacgtgagt
     1201 cctcgcgcgt tgccgcctgg cgtctggggc cgagtggttt gagtaccgaa gatagccgac
     1261 gcatctcttt tcacattcgt tttaatcgtc cgtcatcgct gtttgctcgc tgctggctgt
     1321 tggtggaagg ggaaacagaa acctgggtta tcaatgaact ggcgcgtcag tgcggacatc
     1381 attttgatgc cgaagggatc aaggtcattg agtttgccca gtccgggcta aagccactgg
     1441 ttaaatttgc ccggcgaatg gggattgaat ggcatgtact ggtcgatggc gatgaagcag
     1501 ggaagaaata tgccgctacg gtacgcagcc tgttgaataa cgatcgggaa gccgaacgag
     1561 aacatttaac ggcgttaccg gcgctggata tggaacattt tatgtatcgc cagggatttt
     1621 ccgatgtgtt ccaccgcgtg gcgcaaatcc cggaaaatgt accgatgaat ctgcgcaaag
     1681 ttatctcgaa agcgatccat cgctcttcca aacccgatct tgccattgaa gtggcaatgg
     1741 aggccggacg tcgtggtgtg gactccgtac cgacgctgct gaaaaaaatg ttctcacgcg
     1801 tgctgtggct ggcgcgcggt cgggccgact aactacaaaa catcgtggcc atttgtggct
     1861 gaatagcgtc gagcatctca tagcgccgac ggtattcagc ccgtttttta ctggcgattt
     1921 cggcaatctc ttttcgtgct atctgtgctg gaaggcggta atggcgttca gcatcacata
     1981 cgccgccaac cgactcccag aacgcgttgt aatcggcatg gatcttgccc tctttatcgc
     2041 gataacgcag gctgcggtaa atatgcgttt cattgctgac ggcaataatc tgctctacct
     2101 gcaaacgttg ggcaaacaga caggccgctt ccatcacgag gcgtttggga aatagcccgt
     2161 ggcaggcttt cgtcgcattc tggatttcct ggtgtggaat ttcccatttt gcgccttgca
     2221 gtccgccaat aaacatcgtc cttttcccct gatattcaca cagggtaaaa gtgagctctg
     2281 ccagaggaat accttcgctg ttgcggaaca ggatggtgct gtcaccttct ttatccattg
     2341 agatcatcat ggtcagctca agcgtgaact gctcgccgtt tttgccttcc agcttcgcca
     2401 gttgcagccc gggggtattc aaatataagc tgaattcttc cgccgacata cacccgcgga
     2461 gtaacgcata atggtaacgt aacgcctcca gcaattgctt acggctaaga ttcgccgcaa
     2521 ggtaagggcg atgtagacgt acaggcagtc gcggctggcg cgttaacaac acattgagat
     2581 taggccagtg ggaaagttcg ttcatccact caacgcttaa acgcggcata atcaacgagc
     2641 gcagcaaaaa tttctggcga aaactacggc gatgccagaa tttacccggc cgacactgtc
     2701 ctcgtgccag actaagaaaa agtgacaggc tgctgagaga ttcagatggc gtaaaggtcc
     2761 gttcagttag ctgcgacata ttcatgaaat caatggttat acatgacgtc gatttaacca
     2821 ttgcgtatct taaccaaaca tcaatagtgt gattattaac gtaaatttta gtgttttgtt
     2881 gatatttcgt tgaagttaat gacccggatt ggcatatgga gtattcagaa tatttatgaa
     2941 aaagcggaaa accgtgaaga agcgttacgt tattgcgatg gtgatagtca tcgccgggtt
     3001 gattacgtta tggagaattc ttaacgcacc cgtgccgact tatcagacgc tgattgtgcg
     3061 ccccggtgat ttacagcaaa gcgtgctggc gaccggaaag ctggacgcgc tgcgtaaggt
     3121 tgacgtgggc gcgcaggtca gcggtcagtt gaaaacgctg tcggtggcga ttggcgataa
     3181 agtaaaaaaa gaccagcttt taggggttat tgatcctgaa caggctgaaa accagatcaa
     3241 ggaggtggaa gcaacgctga tggagctacg cgcgcagcgg cagcaggcgg aagcggagct
     3301 gaaactggcg cgggtgacgt attcccgtca gcaacgtctt gcacaaacgc aagcggtttc
     3361 acagcaggat ctcgataacg ccgcgacgga gatggctgtg aaacaggcgc aaattggcac
     3421 cattgacgcg caaatcaagc gcaatcaggc ctctctcgat acggctaaaa ccaatctcga
     3481 ttacacccgc atcgttgccc cgatggccgg ggaagtcacg caaatcacca ctctgcaagg
     3541 ccagacggtg attgccgcac aacaagcacc gaacattctg acgctggcag atatgagcac
     3601 catgctggta aaagcgcagg tttctgaagc ggatgtaatc cacctgaagc cggggcaaaa
     3661 agcctggttt acggtgcttg gcgatccact gacgcgctac gaggggcaaa tcaaggatgt
     3721 gctaccgacg ccggaaaaga ttaacgacgc tattttctat tacgcccgtt ttgaagtacc
     3781 caaccccaat ggtttactgc ggctggatat gaccgcgcaa gtgcatattc agctcaccga
     3841 tgtgaaaaat gtgctgacga tccctctgtc ggcgttaggc gatccggttg gcgataatcg
     3901 ttataaagtc aaattgttgc gtaatggtga aacacgcgag cgtgaagtga cgattggcgc
     3961 acgtaacgat accgatgttg agattgtcaa agggctggaa gcgggcgatg aagtggtgat
     4021 tggtgaggcc aaaccaggag ctgcgcaatg acgcccttgc tcgaattaaa ggatattcgt
     4081 cgcagctatc ctgccggtga tgagcaggtt gaggtgctga agggcatcag cctcgatatt
     4141 tatgccggtg agatggtagc gattgtcggc gcttcgggtt ccggtaaatc gaccctgatg
     4201 aatattctcg gctgcctgga taaggccacc agcggcacct atcgcgtcgc cggtcaggat
     4261 gttgccacgc tggacgccga tgcgctggcg caactgcgcc gcgagcattt cggctttatt
     4321 ttccagcgtt accatttgct ttcgcattta accgccgagc agaacgttga agtacccgcc
     4381 gtctatgccg gtcttgagcg gaaacagcgg ctgcttcgcg cccaggagtt actgcaacga
     4441 ctggggctgg aagaccgtac agagtattac ccggcacagc tttcgggtgg ccagcaacag
     4501 cgcgtcagta tcgcgcgggc attgatgaac ggcggtcagg taattcttgc cgatgaaccg
     4561 accggcgcac tggacagcca ttctggcgaa gaggtgatgg cgatcctgca tcagctgcgc
     4621 gatcgcgggc atacggtgat tatcgtcacc cacgatccgc aggttgctgc tcaggccgag
     4681 cgggtgatcg aaattcgcga cggcgaaatt gtgcgcaatc ctcccgccgt tgaaaaagtg
     4741 aatgctactg gcggaacgga acctgttgtc aacacggcgt ctggctggcg gcagtttgtc
     4801 agcggtttta acgaggcgct gacgatggca tggcgggcgc tggcagcgaa taaaatgcgt
     4861 actttactga ccatgctggg gattattatc ggtattgcgt cggtggtttc cattgtcgtg
     4921 gtgggcgacg ccgccaaaca aatggtgctg gcggatattc gttctattgg tacgaatatt
     4981 attgatgtct atcccgggaa agattttggc gatgacgatc cgcaatatca gcaggcgctg
     5041 aagtacgacg acttaatcgc catccaaaaa caaccgtggg ttgcctctgc cacgcccgcc
     5101 gtctcgcaaa acctgcgcct gcgttataac aatgttgatg tggctgccag tgccaatggt
     5161 gtgagcggcg attattttaa tgtctatggc atgaccttca gtgaagggaa cacctttaat
     5221 caggagcagc tgaacggtcg tgcgcaggtc gtggttctcg acagtaatac tcgccgccag
     5281 cttttccccc ataaagcaga tgtggttggc gaggtgattc tggtcggcaa tatgcccgcc
     5341 agagtcattg gtgtggcgga agaaaaacag tcgatgtttg gtagcagtaa agtgctgcgt
     5401 gtctggctac cttacagcac gatgtccggg cgagttatgg gccagtcgtg gctgaactcc
     5461 attactgtca gggtgaaaga aggatttgac agcgctgagg cggaacagca actcacgcgt
     5521 ttactttcac tgcgccacgg taagaaggat ttctttacct ggaacatgga tggcgtcttg
     5581 aaaaccgttg aaaagaccac acgtacttta caactgtttc tgacgctggt tgcggtgatt
     5641 tcgctggtgg tgggcggtat tggtgtaatg aatattatgc tggtgtcagt gaccgagcgg
     5701 acgcgggaaa ttggcattcg catggctgta ggtgcgcgag caagcgatgt tttgcaacag
     5761 tttttgatcg aagccgtact ggtttgcctg gttggtggcg cgttgggaat aacactgtca
     5821 ctgttaattg ctttcacctt gcagcttttc ttacccggct gggagattgg tttttcaccg
     5881 ttggcactgc tgctggcgtt tctctgctcg acggtcaccg ggattttatt tggctggtta
     5941 cccgcacgaa atgcggcacg actggatcca gtagatgctc tggcacgaga gtaatttttg
     6001 agataaaaat gccagccgat cgggctggca ttttgccttt aggatgtaca caatgagaca
     6061 gaagagctac gcgactgccg cttctacttc gacgggcaca ataacactgg cgtgattgcc
     6121 ttttggcccc tggtggacat caaactgaac ggattgtcca gcttttagcg ttctgtaacc
     6181 atccatctga atggtggaat aatgagcgaa aatatcttcg ccgccgcctt cagggcagat
     6241 gaaaccaaac cctttggcat tgttgaacca cttgacagta cccttttcca tgcttcgaca
     6301 tccttcgcaa atcttataca agtaagatgg aataaaccgg ggtcagagag ggggctgttc
     6361 aaaacctcgc caactctaga aatacaattt agagaattag ggcgagccgt caagcatttg
     6421 acaggggcca gggggcaggt atgaatcaaa aatttgaagc agttaacgct attgacagga
     6481 atgtgacaga tgtcgctgat gccaacgata gatgatagtt atctatcatg tggagtagat
     6541 tggtcaggca aataagctct tgtcagcggc agggcgttct gccgataacc gtaaccgaag
     6601 atgataactg acaatgggta aaacgaacga ctggctggac tttgatcaac tggcggaaga
     6661 aaaagttcgc gacgcgctaa
//
LOCUS       DAZFRG010000244         6635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFRG010000244 DAZFRG010000000
VERSION     DAZFRG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.245"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(15..134)
                     /locus_tag="ACHKUC_004119"
                     /pseudo
     CDS             complement(15..134)
                     /locus_tag="ACHKUC_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            212..1306
                     /gene="ddlA"
                     /locus_tag="ACHKUC_004120"
     CDS             212..1306
                     /gene="ddlA"
                     /locus_tag="ACHKUC_004120"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6620277.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1330..1542)
                     /gene="yaiZ"
                     /locus_tag="ACHKUC_004121"
     CDS             complement(1330..1542)
                     /gene="yaiZ"
                     /locus_tag="ACHKUC_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO6620278.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1802..2110
                     /gene="yaiY"
                     /locus_tag="ACHKUC_004122"
     CDS             1802..2110
                     /gene="yaiY"
                     /locus_tag="ACHKUC_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO6620279.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2169..3263)
                     /gene="yaiW"
                     /locus_tag="ACHKUC_004123"
     CDS             complement(2169..3263)
                     /gene="yaiW"
                     /locus_tag="ACHKUC_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO6620280.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3276..4496)
                     /gene="sbmA"
                     /locus_tag="ACHKUC_004124"
     CDS             complement(3276..4496)
                     /gene="sbmA"
                     /locus_tag="ACHKUC_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO6620281.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDKVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4848..6005
                     /gene="ampH"
                     /locus_tag="ACHKUC_004125"
     CDS             4848..6005
                     /gene="ampH"
                     /locus_tag="ACHKUC_004125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO6620282.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGTRVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6006..6629)
                     /gene="iprA"
                     /locus_tag="ACHKUC_004126"
     CDS             complement(6006..6629)
                     /gene="iprA"
                     /locus_tag="ACHKUC_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO6620283.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRNGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLCREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1682 a         1715 c         1594 g         1644 t
ORIGIN      
        1 tcaccacatg gtcatttaag ccaggaataa attagcttcc cgttcatcat ttttgtgatc
       61 ttgcacacat ctttgagata acgcactttc ttcaccagta cgttgctcgc tttgtcagca
      121 gaagattgca acattctgtc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg
      181 cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt
      241 tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc
      301 cattgataaa agtcgcttcg atgttgtgct gctgggcatt gataaacaag ggcaatggca
      361 cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg
      421 cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc ttattgacgc
      481 acaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct
      541 gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc
      601 tgatgttctg gcttcagcgg cctgtatgga taaagatgtc accaaacgtc tgctgcgcga
      661 tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag
      721 ttttgccgaa gtggagtcta aactggggtt accgctgttt gtaaaaccgg ctaatcaggg
      781 ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacacaattg ccgtcgatct
      841 ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc gtgaaatcga
      901 atgcgcagtt cttggcaacg ataacccgca agcaagcacc tgtggcgaga tcgtactcac
      961 cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggtagt
     1021 tccggcagcc attgcgccag aaatcaacga taagatccgg gcgattgccg ttcaggctta
     1081 tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga
     1141 agtggtgatc aacgagatca acaccctgcc tggcttcacc aacatcagta tgtatccgaa
     1201 gctgtggcaa gccagtggtc tgggttacac cgacctgatc acgcgtttga ttgagctggc
     1261 gctggagcgt cacgctgcgg ataacgcact gaaaaccaca atgtaatatt caaacgcccg
     1321 gtaatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcaaccccgc
     1381 cagccagaaa ctaatcaccc atgtcaccag accgacggca taggtctgcc aaccttttgc
     1441 ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc
     1501 atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc cgttaacgca
     1561 aaacaacatc cttgcatatt ctgtaacaac tgtctgtggt gtagacaaca ctttcgcaat
     1621 tgttaataaa tttaacttaa ggttgctatt ttgtgactca caacgaagat tcacgcttcg
     1681 ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa tgacctgtca
     1741 gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt
     1801 tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc
     1861 aacgcctggc aatattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcaca
     1921 attgctgaac ctgctagtgc atgcgcccgg cgtctatgag cgtttaatgc aggtccagga
     1981 aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accatttttg ggctgatccc
     2041 gtttttagta ggctgcctca tttttgcagt agtggcgcta tggctgcact ggcgacatcg
     2101 ccgccagtaa gatttacgtt gccagatgcg gcctaaaacg ccttatctgg cgtgacattc
     2161 ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt cgcaaaccag
     2221 gccgtagtca gattgcgggt gattttcggg ctttccagtt gaatgccagg caacatttcg
     2281 cggggtagtg atttaccggt tttcgcttcg gcaagttgat aaactttctt gtacagcgcc
     2341 gtctcctcaa aagagaagct gtcccctttc tctaactgac ggcgaatttc gctgtcgctc
     2401 atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg ctctttactg
     2461 tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt actgaccgcg
     2521 ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc aaaacggtat
     2581 atcggcgcgc tatagctagc gggataattc agtaaatggt aagtaccaaa ccacaacccg
     2641 ccgcggcgac tgaagacttc ctgacggact gtaccgtcca ttttccacgg ataccctttg
     2701 gtatgctgtt cggcaaaagc aatactgacc tgcatcggcc caccggtgcg caccggattg
     2761 agcgaaccaa acagtgtctg ccccattggc accatgctga tcagatcgtc aaaaatcgcg
     2821 ctcaattgct tttctgtacg caccgaatct aaccgttcgc tatagctttt gccgtttggc
     2881 gatttgattt ttagcgcggt atgcaccaga aaagcaggaa tgtgcatccg ttcagcacga
     2941 cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc tgcctgataa
     3001 tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt cggtgccagc
     3061 ccctgacttt caaaggtggt ggcgagatct ttcgcccagg catcgcggtc tttcacgctc
     3121 gcaggcatct tctgccgcac gacgctcgcc acatctatcg ctttttcgcc ctttttcaac
     3181 ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa tgacagcgaa
     3241 gatagaggat tgacgcgcga cattacttct cctttttagc tcaaggtatg ggttacttcc
     3301 tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta gatagacatc
     3361 aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg aacctgacca
     3421 aaaacgttag taatctgcgt catcaggccg agtgtaatcg taccggcaac aattgacgga
     3481 aacagcaaga acaaaccgaa aaccttatcg acctgcaaat agagaatgcg ggcgatattg
     3541 aaatacatat agtgaaaata gaggcggaaa tagtttttcc gtacggcgct aaaaagctcg
     3601 cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag ctctttacgg
     3661 taggcagcct ctacacgctg gtttttaaac tccagccccg gcagtttgat ccctaccact
     3721 gccagtaatc cggtccccat cagcgaccag acgatagcgg caatcaccag accatacgga
     3781 atgtgcccga caatcggcag ctccggcaca tgtgcggaga gcgttaccag caccggcagg
     3841 aaggcgatca acgtcatgat agcgttgata aagctgacgc ccatattctc cagcgttgaa
     3901 gcaaaacgca tggtgtcttc ctgcacacgc tgcgcggccc cttcgatatg acgcagttgt
     3961 tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac gtagtgactg
     4021 acaaagaagt tgttcagcac actgatcacc accgcgatca gcgcaatccc cagaaagacg
     4081 cccacttcgc ggtaaaattg ttcgatggtg actttatgcg gcgaacttag cgccgtttga
     4141 atcagatcat agaacggcgc ataccaggcg ttgacggcaa ccccgacttc caccaaaaac
     4201 caggtgacga agataatcag cgcagtaccg agtattgacc agtattgcca acggtgcggg
     4261 ctatagataa accagaacaa tgcaaaaagt cctacgcaaa caatgtagta agcgtaaaaa
     4321 atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc ggaagcgccg
     4381 gtgatacgcg ccacccaatc acccccaccg gcttgccaga agataacggc aatcaatgcc
     4441 caaacaaagg ccgagagaaa aaacgttccc ggctttggga aaaaagactt aaacatatgc
     4501 actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat gaccgctaca
     4561 aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta gttcgccagc
     4621 ctgaccataa gaattgtttc gacgggtttc acaaaccaaa gattgtttac ataagcggat
     4681 taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc gtgtttacgc
     4741 gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc atcaatgagt
     4801 agtataaata cgctcagtta ccttcattca atctatggac accaccgttg aaacgtagtc
     4861 tgcttttttc tgccgtgctg tatgcggcgt cattgacctc cgtccatgcg gcgcagccga
     4921 tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat attttttacg
     4981 gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc gtctttcgca
     5041 gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcc gtcatacgta
     5101 ttgcttccct caccaagtta atgaccagtg agatgctggt gaaattgctc gaccagggga
     5161 ccgtgaagtt aaacgatccg ctaagtaaat atgctccacc aggcacacgt gtaccgacct
     5221 acaatgggac gcccattacg ctggtgaatc tggcaaccca taccagcgcc ctgccccgtg
     5281 aacagcccgg tggcgcggca catcgtccgg tctttgtctg gccaacgcgc gagcaacgct
     5341 ggaaatacct ttccactgcc aaactaaaag ccgcgccggg cagccaggcc gcgtactcca
     5401 acctcgcatt cgatctgctg gcagatgcac tggcgaatgc gtccggcaag ccttataccc
     5461 agttgtttga agagcagatt acccgtccgt tggggatgaa agacaccacc tacaccccct
     5521 caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg tgcaataaca
     5581 cgctggcggc aattggcagc ggcggcgtct attccacgcc aggcgatatg atgcgctgga
     5641 tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggctgat cgcatgcaga
     5701 cgttgattta tcagcgcgcg cagtttacaa aagtgattgg catggatgtc cccggcaaag
     5761 cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt ccggggatta
     5821 ttcagaagac aggtggtggt ggcggtttca ttacctatat ggcgatgatc ccgcagaaga
     5881 atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa aatatgagcg
     5941 atggcatcaa tgacctggtg accgagctaa gcgggaataa accgttggtt atccccgcat
     6001 cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt attcatttcg
     6061 atatagccgc ctttacgcaa agcagcgaga acttccgcaa cgacagaacg cgatatacgt
     6121 gtacgttgat ggatataatt catcacgcca atacgtgatc gcaattcttc attccagtca
     6181 atcattgata ataacgtcgc gcggatttgt tcgtaggaat tatgcccaat gagctgaacg
     6241 tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc tcgccagagt
     6301 tgattttgtt caataagagt aatggtttgt ttggctggca aatgatatcc cgtacagttt
     6361 ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc cagccccata
     6421 atataaggag cctgtgtaat accgataagt acgttttctt ctctacacaa agagataacg
     6481 ccctctaaaa taacgaaagt atcttcgcta ttgacatcac tggaaaatat aacttgcttt
     6541 tcattattaa actcgaagcg cgtaccgttt ctggacaaac atttatcgag cttaccaaat
     6601 tcctgaagag gtttaactac agataacatt tgcgc
//
LOCUS       DAZFRG010000245         6604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFRG010000245 DAZFRG010000000
VERSION     DAZFRG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.246"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(298..2331)
                     /gene="betT"
                     /locus_tag="ACHKUC_004127"
     CDS             complement(298..2331)
                     /gene="betT"
                     /locus_tag="ACHKUC_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO6620284.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDS"
     gene            2460..3047
                     /gene="betI"
                     /locus_tag="ACHKUC_004128"
     CDS             2460..3047
                     /gene="betI"
                     /locus_tag="ACHKUC_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO6620285.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAERRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            3061..4533
                     /gene="betB"
                     /locus_tag="ACHKUC_004129"
     CDS             3061..4533
                     /gene="betB"
                     /locus_tag="ACHKUC_004129"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO6620286.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGCQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPHTNFGPLVSFPHR
                     DNVLRYIAKGIEEGARVLCGGDVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4547..6235
                     /gene="betA"
                     /locus_tag="ACHKUC_004130"
     CDS             4547..6235
                     /gene="betA"
                     /locus_tag="ACHKUC_004130"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO6620287.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIFFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKDALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1626 a         1811 c         1782 g         1385 t
ORIGIN      
        1 accttaaaca acaaacggtt acattgattt tagttgattt gcgctacgcg ttccccatga
       61 ataaagcgtc atttcaaatt agcaccaata tttacattaa ataagactaa gcttattgaa
      121 cattcacaac agtccaaacg taggttttgg gcttcaatag ttcttttctt ttttattatc
      181 tgttgcctgg caatcatcaa tcgatactaa gggcttaact gtacacagat ataacgtatc
      241 aagaggtaag agaaaccgcg ccatatgcag acatggcgcg gttttatgca ataacaatca
      301 cgagtccggg aacatcacgc tatggcccgg cgcttcacga tggagatgga taaagttaag
      361 gtgccgctcg tactggtcga gaatatcggt gatcacctgc tctttgctgt agtccatcag
      421 gtcgttgccc tggctgcctt ctaacaggaa ggtttccagc cggtagtagg tcgatttccc
      481 gctacgtgcg cggtaggtaa agcccggcac tgaatattgc tgcggccaaa tctgatagac
      541 aaagttctgc tcttcgccca tatgcaccaa caaatccaga tgccccaact gttgtccctc
      601 ttccggcggc aggtttttta gctccacgta cgcgccgcgc aaccgcagct cttgcgccac
      661 ttcttccatt gccgggtaac agaccgtctc catcatctgt ttagtgtaac gcgtgcccgg
      721 ataattcatc aggcgcgaga gacgtttttt ccagctcagg cgatcctgaa gccccagcgg
      781 tcgcggcgcg gtatcacggt tggcactttc acggcggtaa tcttctacct tcagagattt
      841 atacaacccc gccatcacga agaaaatcac aaagctgaac ggcagcccca tgattaccgt
      901 ggtgttttgc agcgcggata tcccgttggt catcagcatg ccaagcgtca gcagaccaat
      961 cgccaccgac cagaagacgc gcagccagcc gggggcgtcg ctgttgatat ctttaagctg
     1021 cgaggtgaaa ttccccagca ccagcgcccc ggagtccgcc gaggtcacat aaaacagcag
     1081 gccagtaatg gtggcgacgg aggcgctaaa ggtaaacgcc ggatactgcg ccagcaggct
     1141 gtagaagccg cgctccggat ggaccatcgc ttcctcggca aatgccgcgc cgccgtggat
     1201 gatttcatac agcgcgctat tgccgaacac cgagagccat aacagcgtga aggtaaacgg
     1261 aataatcaac gtgcccagca cgaactggcg aatggtacgc ccacgcgaga tacgcgccag
     1321 gaacaagccg acaaacggcg accatgccac ccaccatgcc cagaagaaga gcgtccagtt
     1381 attcatccac tcaaccggac ggtcgaaggc aaaactgttg agcgtcatgc ccataaagcg
     1441 attcacatag tcgccaacat tcagcaccag tgcattaagc aggaacgagg tgtcgcccat
     1501 aaacaacacg aacagaatca atcccagcgc cagcgcaacg ttgagttccg ataacacgcg
     1561 aatgccctta tcaacaccgg atgtcaccga gatagtggcg atgatcaccg acaaggcgat
     1621 cagtgccgct ttcgccgcca tcgaatcggg aatatcaaac agcacgctca agccatagtt
     1681 aagctgtacc acaccgatac cgagcgtagt ggcaataccg aagatggtgc cgatcaccgc
     1741 agcaatatcc actgagtgac ctatcggccc gttaatccgt ttaccgaaga tcgggtacag
     1801 cgccgagcgg atagtgagcg gcaaattata acgatagcta aagtatccga gcgccatgcc
     1861 catcagcgca tacatcgacc agccggttaa gccgtagtga aacagcgtcc agaccatcgc
     1921 ctgacgcgcg gcctcaatcg tctgtcccgc gccttccggc ggctgcatat actgcgttac
     1981 cggttcggct acggagaaga acatcaggtc gataccgatc ccggcagcaa acagcatcgc
     2041 cgcccaactc agcagactga attccggttt tgattgttct ggcccgagct tcaccgaacc
     2101 aaaacgcgaa caagcgatac agaccacaaa gacaatatag agcgttgccg ccagcagata
     2161 gtaccaaccg aaggttttag aaacccagtc cagcgtgcgg ccaatccaca gggccgagaa
     2221 gtcgcgaaac aggatcgttg tcagggaaaa caacaaaatc agtccggcgg aagtgtaaaa
     2281 caccaccgga ttgattttgt ccttttccct gctgtgtgaa aggtctgtca tccagtatcc
     2341 ccactgttat tgttaccatt taaaatcaaa ttagtaacaa ttaagacaca ttttatattg
     2401 aacgtccaat caataaccgc tttaatagat aaacaacgct gatgaatgga gtggcgaaaa
     2461 tgcccaaatt gggaatgcag tcgatccggc gcagacaact gatcgacgcc acgctggaag
     2521 caataaatga agtgggcatg cacgatgcaa cgatcgcgca gatcgcccgc cgtgcaggcg
     2581 tttctacggg gatcatcagc cactatttca gggacaaaaa tggtctgctg gaagcaacca
     2641 tgcgcgatat caccagtcag ctgcgtgacg cggttttgaa tcgattacat gcacttccgc
     2701 agggcagtgc agagcggcga ttacaggcga ttgttggcgg aaacttcgat gaaacccagg
     2761 tgagcagtgc ggcgatgaaa gcctggctgg ccttctgggc cagcagtatg catcagccga
     2821 tgctctatcg cttgcagcag gtcagtagcc gccgcttatt gtcgaatctg gtgagcgaat
     2881 ttcgccgcga gttaccgcgc caacaggcac aggaagcggg ctacggcctt gccgcgctaa
     2941 ttgacggatt atggctgcgc gcggcactga gcggcaaagc gctggataaa cctctcgcac
     3001 actcgctgac ccgtcacttt attactcagc atttacccac cgattaaccg aggaaacgtg
     3061 atgtcccgaa tggcagaaca gcagctttat atacatggtg gttatacctc cgccaccagc
     3121 ggtcgcacct tcgagaccat taacccggcc aacggtaacg tgctggcgac cgtgcaggcc
     3181 gccgggcgtg aggatgtcga tcgcgccgtg aagagtgccc aacaagggca aaaaatctgg
     3241 gcggcgatga ccgctatgga acgctcgcgt attctgcgtc gggccgttga tattctgcgt
     3301 gaacgcaatg acgaactcgc aaaactggaa accctcgaca ccggaaaagc atattcggaa
     3361 acctcaaccg tcgatatcgt taccggtgcg gacgtgctgg agtactatgc cgggctgatc
     3421 ccggcgctgg aaggctgcca gatcccgttg cgtgagacgt catttgttta tacccgccgc
     3481 gaaccgctgg gtgtggtggc ggggattggc gcatggaact acccgatcca gattgctttg
     3541 tggaaatccg cccccgcgct ggcggcgggt aacgcgatga tcttcaaacc gagcgaagtc
     3601 accccgctta ccgcgttaaa gctggctgaa atttacagcg aagcgggcct gccggacggt
     3661 gtatttaacg tgttgccggg cgtgggcgcg gagaccgggc aatatctgac cgatcatccg
     3721 ggcattgcca aagtgtcatt taccggcggc gtcgccagcg gcaaaaaagt gatggctaac
     3781 tcggcagctt cctccctgaa agaagtgacc atggagctgg gcggtaaatc gccgctgatc
     3841 gtttttgacg atgcggatct cgatctcgcc gccgatatcg ccatgatggc gaatttcttc
     3901 agctccggtc aggtgtgtac caacggcact cgcgtcttcg ttccgacgaa atgcaaagcc
     3961 gcatttgaac aaaaggttct ggcgcgcgtt gagcgcattc gcgcgggcga cgttttcgat
     4021 ccgcacacta acttcggccc gctggtcagc ttcccgcatc gcgataacgt gctgcgctat
     4081 atcgccaaag gcatagagga aggcgcgcgc gtactgtgcg gcggcgatgt actgaagggg
     4141 gatagcttcg ataacggcgc atgggttgca ccgaccgtgt tcaccgattg cagcgacgat
     4201 atgaccattg tgcgcgaaga gattttcggg ccggtgatgt ccattctgac ctacgagtcg
     4261 gaagacgaag tcattcgccg cgctaacgat accgactacg gtctggcggc gggtatcgtg
     4321 acggcggacc tgaacctcgc gcatcgcgtc attcatcagc tggaagcggg tatttgctgg
     4381 atcaacacct ggggcgaatc cccggcagag atgcccgttg gcggctacaa acactccggc
     4441 attggtcgcg agaacggcgt gatgacgctc cagagttaca cccaggtgaa gtccatccag
     4501 gttgagatgg ctaaattcca gtccatattc taaccaggag gtttatttgc aatttgacta
     4561 catcattatt ggtgccggct cagccggcaa cgttctcgct acccgtctga ctgaagatcc
     4621 gaacacaacc gtgctgctgc ttgaagcagg cggcccggac tatcgctttg acttccgcac
     4681 ccagatgccc gccgcgctgg cgttcccgct acagggcaaa cgctacaact gggcctatga
     4741 gacggaaccc gaaccgttta tgaacaaccg ccgcatggag tgcggacgcg gcaaaggtct
     4801 gggcggttca tcgctgatca acggtatgtg ctacatccgt ggcaatgcgc tggatctcga
     4861 taactgggca caagaacctg gtctggagaa ctggagttat ctcgactgcc tgccctacta
     4921 tcgcaaggcc gagactcgcg atgtgggcga gaacgactac cacggcggtg atggcccggt
     4981 gagcgtcacc acctccaaac ccggcgtcaa tccgctgttt gaagcgatga ttgaagcggg
     5041 cgtgcaggcg ggctacccgc gcacagacga tctcaacggc tatcagcagg aaggttttgg
     5101 cccgatggat cgcaccgtca cgccgcatgg ccgtcgcgcc agcaccgcgc gtggctatct
     5161 cgatcaggcc aaatcgcgcc ctaacctgac cattcgtact cacgccatga ccgatcacat
     5221 cttttttgac ggcaaacgcg cggtgggcgt cgagtggctg gaaggcgaca gcaccattcc
     5281 gacccgcgcg gcggcgaata aagaagtgct gttatgtgca ggcgcgattg cttcaccgca
     5341 gatcctgcaa cgctccggcg tcggcaacgc tgaactgctg gcggagtttg atattccgct
     5401 ggtgcatgaa ttacctggcg tcggcgaaaa tcttcaggat cacctggaga tgtatctgca
     5461 atatgagtgc aaagaaccgg tttccctcta ccctgccctg cagtggtgga atcagccgaa
     5521 aatcggtgcg gagtggctgt ttggcggcac cggcgttggt gccagcaacc actttgaagc
     5581 aggcggattt attcgcagcc gtgaggaatt tgcgtggccg aatattcagt accatttcct
     5641 gccagtggcg attaactata acggctcgaa cgcagtgaaa gagcacggct tccagtgcca
     5701 cgtcggatcg atgcgctcgc caagccgtgg gcatgtgcgg attaaatccc gcgacccgca
     5761 ccagcatccg gcgattctgt ttaactacat gtcgcacgag caggactggc aggagttccg
     5821 cgacgcaatt cgcatcaccc gggagatcat gcatcaaccg gcgctggatc agtatcgtgg
     5881 ccgcgaaatc agccccggtg tcgaatgcca gacggatgag cagctcgatg agttcgtgcg
     5941 taaccacgcc gaaaccgcct tccacccgtg cggtacctgc aaaatgggtt atgacgagat
     6001 ggcagtggtt gacgccgaag gccgcgtaca cgggctggaa ggcctgcgcg tggtggatgc
     6061 gtcaattatg ccgcagatta tcaccggcaa tttgaacgcc acgacgatta tgattggcga
     6121 gaaaatggcg gatatgattc gcgggaagga tgcgttgccg aggagcacgg caagatattt
     6181 tgtggcaaat gggatgccgg tgagagcgaa aaaaatgagt cgtgatgtga actgactcag
     6241 gaacctatca gattgagagt taccgttcta ctatgaggac cgaatcctta tataaaacac
     6301 tgaggtaact ctcatgcttc atcgtgacaa tctcaccagc aaattctaag ctgacttgct
     6361 caatctggct gatgaaggtg ctatgcatac acagataaac tacagatgaa ataccactca
     6421 gcttaagaat tacgccgcta atgttcctgg ctatggttac ctgttcattc ccgctcacgg
     6481 ttagcatgta cgaaaagggt tcttgtaacg agtacaaaaa taagtcagac caaaaccaca
     6541 tggagcttta aacacggttc gtataattta ttagcagcga tttattttta ctcttcttta
     6601 atta
//
LOCUS       DAZFRG010000246         6549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFRG010000246 DAZFRG010000000
VERSION     DAZFRG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.247"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..830
                     /gene="exuR"
                     /locus_tag="ACHKUC_004131"
     CDS             54..830
                     /gene="exuR"
                     /locus_tag="ACHKUC_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO6620288.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1070..1153
                     /locus_tag="ACHKUC_004132"
     CDS             1070..1153
                     /locus_tag="ACHKUC_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620289.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            1175..1837
                     /gene="yqjA"
                     /locus_tag="ACHKUC_004133"
     CDS             1175..1837
                     /gene="yqjA"
                     /locus_tag="ACHKUC_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO6620290.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1841..2224
                     /gene="mzrA"
                     /locus_tag="ACHKUC_004134"
     CDS             1841..2224
                     /gene="mzrA"
                     /locus_tag="ACHKUC_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO6620291.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2371..2739
                     /gene="yqjC"
                     /locus_tag="ACHKUC_004135"
     CDS             2371..2739
                     /gene="yqjC"
                     /locus_tag="ACHKUC_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO6620292.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKSLSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            2777..3082
                     /gene="yqjD"
                     /locus_tag="ACHKUC_004136"
     CDS             2777..3082
                     /gene="yqjD"
                     /locus_tag="ACHKUC_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO6620293.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3085..3489
                     /gene="yqjE"
                     /locus_tag="ACHKUC_004137"
     CDS             3085..3489
                     /gene="yqjE"
                     /locus_tag="ACHKUC_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6620294.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3479..3778
                     /gene="yqjK"
                     /locus_tag="ACHKUC_004138"
     CDS             3479..3778
                     /gene="yqjK"
                     /locus_tag="ACHKUC_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO6620295.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3964..4356
                     /gene="yqjF"
                     /locus_tag="ACHKUC_004139"
     CDS             3964..4356
                     /gene="yqjF"
                     /locus_tag="ACHKUC_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6620296.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            4426..5412
                     /gene="yqjG"
                     /locus_tag="ACHKUC_004140"
     CDS             4426..5412
                     /gene="yqjG"
                     /locus_tag="ACHKUC_004140"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO6620297.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5695..6063
                     /gene="yhaH"
                     /locus_tag="ACHKUC_004141"
     CDS             5695..6063
                     /gene="yhaH"
                     /locus_tag="ACHKUC_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6620298.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6097..>6549)
                     /gene="yhaJ"
                     /locus_tag="ACHKUC_004142"
     CDS             complement(6097..>6549)
                     /gene="yhaJ"
                     /locus_tag="ACHKUC_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6620299.1"
                     /translation="VIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEV
                     TRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVE
                     KDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT         1633 a         1637 c         1653 g         1626 t
ORIGIN      
        1 catgcataaa gtggtatgat aactttgtcc gcattccact cctggagacg catatggaaa
       61 tcactgaacc acgccgtttg tatcaacaac ttgccgctga cctgaaagag cgcatcgaac
      121 agggcgtcta tctggtgggt gataaactgc ctgccgaacg ctttattgcc gatgaaaaaa
      181 acgtcagccg caccgtggtt cgtgaagcca tcatcatgct ggaagttgaa ggctatgtcg
      241 aagtgcgtaa aggttcaggt attcatgtgg tttccaacca gccgcgccat cagcaggcgg
      301 ctgacaataa tatggaattc gccaattacg gtccgtttga gttgctccag gctcgccagc
      361 tcatcgaaag taatattgcc gagttcgcgg caactcaggt cacaaaacag gacatcatga
      421 aattgatggc cattcaggag caggcacgcg gtgaacaatg cttccgtgat tccgagtggg
      481 atttgcagtt ccatattcag gtagccctgg cgacgcagaa ctccgccctg gcggctatcg
      541 ttgaaaaaat gtggacccag cgtagtcata acccgtactg gaaaaaactg cacgaacaca
      601 ttgatgcccg taccgtcgat aactggtgtg atgaccacga tcaaatcctc aaggcgctga
      661 ttcgcaaaga tcctcatgct gctaagctgg caatgtggca gcacctggaa aacaccaaga
      721 tcatgttatt taacgaaacc agcgacgatt tcgagttcaa tgccgaccgc tatctgttcg
      781 ccgaaaaccc ggtggtgcat ctcgatacag ccaccagcgg cagtaaatga aattcctgtc
      841 cgacaggcgc tacagtgcgc ctgtttgtct cgtcggtaag cgaaaagtat aaagtgtcag
      901 cctgtgtaaa tcctctcgcc tccctcccct gcattcagca aaatcagact ccacggacat
      961 gtaattttga taacgaacaa cgttgacctt tgttacaatt agattcaatt tgaatttatg
     1021 cttttgaatg ctttcttatc tcacgattta acagggaata gttcaggctg tgttgatgta
     1081 tcaaacccgc agaacatacc aagacagcaa taacattgcg gtagtgcatc atttaaaacc
     1141 agcgtggcgt taaccgattc accaggaata atgaatggaa cttttgaccc aattgctgca
     1201 agccctgtgg gcgcaggatt ttgaaaccct ggccaatccg tcgatgattg gcatgttgta
     1261 ttttgtcttg tttgtcattt tgttccttga aaacggcttg cttccggcgg cctttttacc
     1321 gggcgacagt ttactggtac tggtcggcgt attgattgcg aaaggcgcga tgggctatcc
     1381 gcaaacgatt ctgctgctga ccgttgccgc cagcctcggt tgctgggtca gctatattca
     1441 ggggcgatgg ctgggcaata cccgcaccgt acaaaactgg ctatctcatt tacccgcgca
     1501 ttatcatcaa cgcgcacacc atctttttca taaacacggt ttatcggcgc tgttaattgg
     1561 tcgctttatt gcgtttgtca gaacactgct gccgacgatt gccgggttat cagggctgaa
     1621 taacgcgcgc tttcagttct tcaactggat gagtggtctg ctgtgggtgt tgatcctgac
     1681 aactctgggt tacatgctcg gcaaaacgcc ggtattttta aagtacgagg accagctgat
     1741 gtcatgcctg atgctgctcc cggtggtgct gctggtgttt ggcctggcag gttctctggt
     1801 cgtgttatgg aaaaagaaat atggaaatcg ggggtaaggg atgcaaatac ctcgcatgtc
     1861 acttcgccag ctagcctggt ccggcgctgt tttacttctg gtcagcacac tattgctggc
     1921 ctggtccgcg gttcgccagc aagagtctac gctggcgatt cgtgccgttc atcaagggac
     1981 aacgatgcca gacggttttt caatctggca tcaccttgac gctcacggca ttcctttcaa
     2041 aagtatcacc cccaaaaacg acactctgtt aattaccttt gactccagcg accagagcgc
     2101 cgccgcaaaa gcggtcctcg acagaacatt gccccatggc tacatcattg cgcagcagga
     2161 caataacagt caggctatgc agtggctgac ccggttgcgg gataactctc atcgcttcgg
     2221 ataacttcct gttatccgaa acagatcact cactttggtg atttcaccgt aactgtctat
     2281 gattaatgag gcggtggtta acacgaccgc ttcgttgtct ggacaatcgt tcctttgtaa
     2341 taggtccgaa tcacaatgga aggttcaaga atgaaatacc gcatcgcttt agctgtttct
     2401 ctctttgctc ttagtgccgg tagttatgcc actaccctgt gtcaggaaaa ggagcaaaat
     2461 atccttaagg agatcagcta tgccgaaaaa caccaaaacc agaatcgtat tgacggtctg
     2521 aataaatccc tgagtgaagt ccgggccaac tgttcagata gccaactgcg tgccgatcat
     2581 cagaagaaaa tcgcaaagca gaaagatgag gtggcggaac gccagcaaga tttagccgag
     2641 gcgaagcaaa aaggcgatgc agataagatt gccaaacgcg aacggaaact ggcagaagcg
     2701 caggaagagc tgaaaaagct gaaagcgcgc gactactaac tcacaatagt cactacttac
     2761 tcacctggag aaaactatgt cgaaagaaca cactacggaa catctgcgtg ctgagttgaa
     2821 atccctttcc gatacgctgg aagaggtgct tagctcatct ggcgagaagt caaaagaaga
     2881 gttgagtaag attcgtagca aagcggagca ggcactgaaa cagagccgtt atcgcctggg
     2941 tgaaaccggt gatgccattg ccaaacaaac ccgtgtcgcg gcggcgcgtg ccgatgagta
     3001 tgtgcgtgaa aatccgtgga cgggcgtggg cattggcgca gcaatcggtg tagtgctcgg
     3061 cgttctgctg tcgcgtcgtt aattatggcg gacactcatc acgcgcaagg gcccggtaaa
     3121 agcgttctgg gcatcgggca gcgaattgtt tccatcatgg ttgaaatggt ggagacacgt
     3181 ctgcggctgg cggtggtgga gctggaagag gaaaaagcga atctctttca acttttactg
     3241 atgctgggcc tgacgatgct tttcgctgca tttggtctta tgagcctgat ggtgctagtt
     3301 atctgggcgg ttgatccgca atatcgcctg aatgcgatga ttgccaccac cgtggtgttg
     3361 ctgctactgg cactgattgg cggcatctgg acgttacgta aatcgcgtaa gtctacgttg
     3421 ctgcgccaca cgcgccatga gctggcaaac gatcggcagc tgctcgagga ggagtcccgt
     3481 gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg cttagccaga tccagcaaca
     3541 acggctggat ctttccgcca ctcgtcgtga atggctggag gcaacaggcg cttacgatcg
     3601 tcgctggaat atgctgttga gtctgcgctc ctgggcgctg gttggcagta gcgtgatggc
     3661 tatctggacg attcgccatc ctaatatgct ggtccgctgg gccagacgcg gttttggtgt
     3721 atggagcgcc tggcgtctgg tgaaaacaac cctcaagcaa caacagcttc gcggttaact
     3781 tcctctctgg ccggagaaat tccggcctta tccctcaaat tttttgaaaa tatttgacag
     3841 ttttccttgc taacaatcac gcttcgtcac gtttatgatt ctctccatcg acagcaacga
     3901 cgctaatagc gcgcccttgc acaaaaaaca atcagcagcc tgagcggcgc agtggagagt
     3961 atgatgaaaa aattagaaga tgttggtgta ctggtagcgc gtattttaat gccgattctg
     4021 tttattacgg caggctgggg aaaaattact ggctacgcgg gtacccaaca atatatggaa
     4081 gcaatgggcg tccccggctt tatgctgccg ctggtgattc tgcttgagtt tggtggtggt
     4141 ctggcaatcc tgttcggttt cctgacacgt accacagccc tgtttactgc gggctttacg
     4201 ctgctgacgg catttttatt tcacagcaac tttgcagaag gcatgaactc gctgatgttt
     4261 atgaaaaacc tgactatttc tggcggattc ctgctgctgg caattaccgg tccaggcgca
     4321 ttcagcatcg accgcctgct gaataaaaag tggtaagcac gcgctatact taacgataaa
     4381 aaacgagggg gaagctcctc gtttttgcta ttggaggata gaaaaatggg tcaactgatt
     4441 gacggcgtct ggcatgacac ctggtacgat accaaatcca ccggcggtaa atttcaacgt
     4501 tcagcttccg catttcgtaa ctggctcact gccgatggcg cgccaggccc cactggcaaa
     4561 ggcggttttg ccgcagagaa agatcgttat catctctatg tttcactcgc ctgcccgtgg
     4621 gcacaccgca cgctgatcat gcgtaagctc aaaggactgg aaccgtttat ttccgtttcc
     4681 gtagtgaacc cgctgatgct ggaaaacggc tggacctttg atgacagttt tccgggagca
     4741 accggcgaca cgctctatca acatgaattt ctgtatcagc tttatctcca cgccgatcca
     4801 cactacagcg gacgagtgac tgttcccgtg ctgtgggaca aaaaaaacca caccatcgtc
     4861 agcaacgaat cagcagaaat catacgcatg ttcaataccg cgtttgatgc gctgggtgcg
     4921 aaagctggag attactaccc accagccctg caaccgaaaa ttgacgaact taacggctgg
     4981 atttacgaca ccgttaacaa tggcgtgtat aaagccggtt ttgccaccag ccagcaagcc
     5041 tacgacgagg cggtggcgaa agtgtttgaa tcgctggcgc gactggaaca gattttaggt
     5101 cagcaccgtt acctgaccgg caaccagcta accgaagccg atattcgcct gtggaccacg
     5161 ctggtgcgtt ttgatccagt gtatgtgacc cacttcaagt gtgataagca ccgcatcagc
     5221 gattacctga atctgtatgg tttcctgcgc gatatctacc agatgccggg aattgccgaa
     5281 acagtcaatt tcgatcacat ccgtaatcat tacttccgca gccataagac catcaaccct
     5341 acgggaatta tttcaattgg tccgtggcag gatctcgatg aaccgcatgg acgagatgtt
     5401 cgcttcggtt aaaaaaaggc accttacgag gtgcctctga taattagcta agtacgtgtt
     5461 gttcatatcg gatgcggcgt aaacgcctta tcaaacctac aaaacattta tattcaatat
     5521 gttgcaatga ccgcgcaggc cagataagcg tagcgcatct ggcaattttg cgcttgccct
     5581 tatccttcac agcgccttat taattcatcg cgataatatt tactgccatt tcattcacgt
     5641 ctattcttaa tttgctgctt taaaaacaag tgattgagca aaattgaggc aaaaatggac
     5701 tggtatctga aagtactaaa aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag
     5761 tactggatgt ttattctggt caacatcatc tttacgttcg tgctggggct actggataaa
     5821 atgttaggct ggcaacgtgc cggtggcgaa ggcatcctga cgacaatcta cggtattctg
     5881 gtgtttttac catggtgggc agttcagttc cgccgcctgc acgacaccga ccgctcggcg
     5941 tggtgggcac tgctattctt aatcccgttc atcggctggc ttattatcat cgtctttaac
     6001 tgccaggcgg gtacgccagg cgaaaaccgc tttggaccag atccgaaact ggaacaagaa
     6061 taataattaa gcaccttgta aaaggtgctt aatcgattat tttccgctaa aaagtttggg
     6121 aatttcccgc agacaccagg atttcgcctc ccccatactg tcacgacgcc aggccataat
     6181 aatatcgatc tcgctggtcg attccgggct gacgacacgc aaccgccctt ccgcaatatc
     6241 tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta atgcctgacg
     6301 tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcaact gtacggtcaa
     6361 caccgggcgc tcacgagcgg tatccgccac cgcaattcca cgatatttca cgcgcgtcac
     6421 ttcagataac ggttccggct cctgatgaat cgggtgatcc ggcgcggcaa catagacgtt
     6481 cattaaggta tagagtttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg
     6541 cgcgataac
//
LOCUS       DAZFRG010000247         6521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFRG010000247 DAZFRG010000000
VERSION     DAZFRG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.248"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..1174
                     /gene="cyoA"
                     /locus_tag="ACHKUC_004143"
     CDS             227..1174
                     /gene="cyoA"
                     /locus_tag="ACHKUC_004143"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO6620300.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1196..3187
                     /gene="cyoB"
                     /locus_tag="ACHKUC_004144"
     CDS             1196..3187
                     /gene="cyoB"
                     /locus_tag="ACHKUC_004144"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO6620301.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3177..3791
                     /locus_tag="ACHKUC_004145"
     CDS             3177..3791
                     /locus_tag="ACHKUC_004145"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO6620302.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3791..4120
                     /locus_tag="ACHKUC_004146"
     CDS             3791..4120
                     /locus_tag="ACHKUC_004146"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO6620303.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4132..5022
                     /gene="cyoE"
                     /locus_tag="ACHKUC_004147"
     CDS             4132..5022
                     /gene="cyoE"
                     /locus_tag="ACHKUC_004147"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO6620304.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5171..>6521
                     /locus_tag="ACHKUC_004148"
     CDS             5171..>6521
                     /locus_tag="ACHKUC_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620305.1"
                     /translation="MNDYKMTSGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPDNVMADEKLEKSLLTTPGVKDVLLAKLEHSVYVKIDSKITNRFEIERV
                     "
BASE COUNT         1387 a         1627 c         1742 g         1765 t
ORIGIN      
        1 ttgattatac caattgccca cccaaactcc ttctgcactc ccctttcgtt ataacgccct
       61 tttgcaacag cttcttaaaa tcaacttgat atgttttgca acatatgtga cctgacagcc
      121 aaatccaagt aacagaaatt taatcatgtt tacagtaatg taaccttccc gtaaaatgcc
      181 cacacacttt aaacgccacc agatcccgtg gaattgaggt cgttaaatga gactcaggaa
      241 atacaataaa agtttgggat ggttgtcatt atttgcaggc actgtattgc tcagtggctg
      301 taattctgcg ctgttagatc ccaaaggaca gattggtctg gagcaacgtt cactgatact
      361 gacggcattt ggcctgatgt tgattgtcgt tattcccgca atcttgatgg ctgttggttt
      421 cgcctggaag taccgtgcga gcaataaaga tgctaagtac agcccgaact ggtcacactc
      481 caataaagtg gaagctgtgg tctggacggt acctatctta atcatcatct tccttgcggt
      541 actgacctgg aaaaccactc acgctcttga gcctagcaag ccgctggcac acgacgagaa
      601 gcccattacc atcgaagtgg tttccatgga ctggaaatgg ttcttcatct acccggaaca
      661 gggcattgca accgtgaatg aaatcgcttt cccggcgaac actccggtgt acttcaaagt
      721 gacctccaac tccgtgatga actccttctt cattccgcgt ctgggtagcc agatttatgc
      781 catggccggt atgcagactc gcctgcatct gatcgccaac gaacccggta cttatgacgg
      841 tatctccgcc agctacagcg gcccgggctt ctcaggcatg aagttcaaag ctattgcaac
      901 accggatcgc gccgcattcg accaatgggt cgcaaaagcg aaacagtcgc cgaacagcat
      961 gtctgacatg gcagcgttcg aaaaactggc cgcgcctagc gaatacaacc aggtggaata
     1021 tttctccaac gtgaaaccag acttgtttgc cgatgtgatt aacaagttta tggctcacgg
     1081 taagagcatg gacatgaccc agccagaagg tgagcacagc gcacatgaag gtatggaagg
     1141 catggacatg agccacgcgg aatccgccca ttaaaggggt tgaggaagaa taaagatgtt
     1201 cggaaaatta tcacttgatg cagtcccgtt ccatgaacct atcgtcatgg ttacgatcgc
     1261 tggcattatt ttgggaggtc tggcgctcgt tggcctgatc acttacttcg gtaagtggac
     1321 ctacctgtgg aaagagtggc tgacctccgt tgaccataaa cgtctcggta tcatgtatat
     1381 catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac gccattatga tgcgtagcca
     1441 gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca cctcaccact acgatcagat
     1501 cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg atgcctttcg ttatcggtct
     1561 gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac gttgcgttcc cgttcctcaa
     1621 caacttaagc ttctggttta ccgttgttgg tgtgattctg gttaacgttt ctctcggcgt
     1681 gggcgaattt gcgcagaccg gctggctggc ctatccaccg ctatcgggaa tagagtacag
     1741 tccgggagtc ggtgtcgatt actggatatg gagtctccag ctatccggta taggtacgac
     1801 gcttaccggt atcaacttct tcgttaccat tctaaagatg cgcgcaccgg gcatgaccat
     1861 gttcaagatg ccagtattta cctgggcatc actgtgcgca aacgtactga ttattgcttc
     1921 cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg gatcgctatc tgggcaccca
     1981 tttctttacc aacgatatgg gtggcaacat gatgatgtat atcaacctga tttgggcctg
     2041 gggccacccg gaagtttaca tcttgatcct gcctgtattc ggtgtgttct ccgaaattgc
     2101 ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg ctggtatggg caaccgtctg
     2161 tatcaccgtg ctgtcgttca tcgtctggct gcaccacttc tttacgatgg gtgcgggcgc
     2221 gaacgtaaac gccttctttg gtatcaccac gatgattatc gccatcccta ccggggtgaa
     2281 gatcttcaac tggctgttca ccatgtatca gggccgcatc gtgttccatt ctgcgatgct
     2341 gtggaccatc ggttttatcg tcaccttctc ggtaggcggt atgactggcg tgctgctggc
     2401 agtaccgggc gcggacttcg ttctgcataa cagcctgttc ctgattgcgc acttccataa
     2461 cgtgatcatc ggcggcgtgg tcttcggctg cttcgcaggg atgacctact ggtggcctaa
     2521 agcgttcggt ttcaaactga acgaaacctg gggtaaacgc gcgttctggt tctggatcat
     2581 cggcttcttc gttgccttta tgccactgta tgcgctgggc ttcatgggca tgacccgtcg
     2641 tttgagccag cagattgacc cgcagttcca caccatgctg atgattgcag ccagcggtgc
     2701 ggtactgatt gcgctgggta ttctctgcct cgttattcag atgtacgttt ctattcgtga
     2761 ccgcgaccag aaccgtgacc tgactggcga cccgtggggt ggccgtacgc tggagtgggc
     2821 aacctcttcc ccgcctccgt tctataactt tgccgttgtg ccgcacgttc acgaacgtga
     2881 tgcattctgg gaaatgaaag agaaaggcga agcgtacaaa aagcctgacc actatgaaga
     2941 aattcatatg ccaaaaaaca gcggtgccgg tatcgtcatt gcggctttct ccaccatctt
     3001 cggtttcgcc atgatctggc atatctggtg gctggcgatt gttggcttcg caggcatgat
     3061 catcacctgg atcgtgaaaa gcttcgacga ggacgtggat tactacgtgc cggtggcaga
     3121 aatcgaaaaa ctggaaaacc agcatttcga tgagattact aaggcagggc tgaaaaatgg
     3181 caactgatac tttgacgcac gcgactgccc acgcgcacga acacgggcac cacgatgcag
     3241 gcggaaccaa aatcttcgga ttttggatct acctgatgag cgactgcatt ctgttctcta
     3301 tcttgtttgc tacctatgcc gttctggtga acggcaccgc aggtgggccg acaggtaagg
     3361 acattttcga actgccgttc gttctggttg aaactttctt gctgttgttc agctccatca
     3421 cctacggcat ggcggctatc gccatgtaca aaaacaacaa aagccaggtg atctcctggc
     3481 tggcgttgac atggttgttt ggtgccggat ttatcgggat ggaaatctat gaattccatc
     3541 acctgattgt taacggcatg ggtccggatc gcagcggctt cctgtcagca ttctttgcgc
     3601 tggtcggcac gcacggtctg cacgtcactt ccggtcttat ctggatggcg gtgctgatgg
     3661 tgcaaatcgc ccgtcgcggc ctgaccagca ctaaccgtac ccgcatcatg tgcctgagcc
     3721 tgttctggca cttcctggat gtggtttgga tctgtgtgtt cactgttgtt tatctgatgg
     3781 gggcgatgta atgagtcatt ctaacgcaag cagcggcgca tcccatggca gcgtaaagac
     3841 ctacatgaca ggctttatcc tgtcgataat cctgacggta attccgttct ggatggtgat
     3901 gacaggggct gcctctccgg ccgtaattct gggaacaatc ctggcaatgg cagtggtaca
     3961 gattctggtg catctggtgt gcttcctgca catgaatacc aaatcagatg aaggctggaa
     4021 tatgacggcg tttgtcttca ccgtgctaat catcgctatc ctggttgtag gctccatctg
     4081 gattatgtgg aacctcaact acaacatgat gatgcactaa gagcggcggt tatgatgttt
     4141 aagcaatacc tgcaagtaac gaaaccaggc atcatctttg gcaacctgat ctcggtgatt
     4201 gggggattcc tgttggcctc aaagggcagc attgattatc ccctgtttat ctacacgctg
     4261 gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta acaactacat cgacagggat
     4321 atcgacagaa agatggaaag gacgaagaat cgggtgctgg ttaaaggcct gatctctcct
     4381 gctgtctcgc tggtgtacgc caccttgctg ggtattgctg gctttatgct gctgtggttt
     4441 ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct ttgtggttta tgtcggcgtt
     4501 tatagcctgt acatgaaacg tcattctgtc tacggcacct tgattggttc gctctccggc
     4561 gctgcgcctc cggtgatcgg ttactgtgcg gtaaccggcg agttcgatag cggcgcagcg
     4621 atcctgctgg ctatcttcag cctgtggcag atgcctcact cttatgccat agccattttc
     4681 cgctttaagg attatcaggc ggcaaacatt ccggtactgc cggtggtgaa aggcatttcg
     4741 gtggcgaaga atcacatcac gctgtatatc atcgcctttg ccgttgccac gctgatgctc
     4801 tctcttggcg gctacgctgg gtataaatat ctggtggtcg ccgctgcggt gagcgtctgg
     4861 tggttaggca tggctctgcg cggctataaa gttgctgatg acagaatctg ggcacgcaag
     4921 ctgttcggtt tctctatcat cgccatcact gccctctcgg tgatgatgtc cgttgatttt
     4981 atggtgccgg actcgcatac gctgctggct gctgtgtggt aacacaacct ctcagttaaa
     5041 aaggtgctac ggcacctttt ttcttagcat tagaatcata tccctctcga aatatttact
     5101 aaaaaatccg catgtttacc ccattcgttt gccgctttac actagtcgcg aatttaaaac
     5161 agaggtggta atgaacgatt ataaaatgac gtcaggtgag cggcgcgcga cctggggttt
     5221 aggcaccgtt ttctcgttgc gcatgctggg catgttcatg gttctgccgg ttctgaccac
     5281 gtacggcatg gctctgcaag gtgccagcga agcattaatc ggtattgcca ttggtattta
     5341 tggtctgact caggccgttt ttcagattcc gtttggcctg ctttcagacc gcattggtcg
     5401 caaaccatta attgtcggtg ggctggcggt gtttgccgcc ggtagcgtta tcgctgcgct
     5461 ctctgactcc atctggggaa ttattctggg ccgggcgcta caaggctccg gtgcgattgc
     5521 cgctgccgtt atggcgctgc tttccgatct cacgcgcgaa caaaaccgca ccaaagcgat
     5581 ggcgtttatc ggcgtgagct ttggcattac ctttgccatt gcgatggtgc ttggcccgat
     5641 catcactcac aaacttgggc tgcacgcgct gttctggatg atcgctattc tggcaacaac
     5701 cggcattgcg ttgaccattt gggttgtgcc caacagtagc actcacgtac ttaatcgtga
     5761 gtccggaatg gtgaaaggca gtttcagtaa agtgctggcg gaaccgcggc tgctgaaact
     5821 caactttggc attatgtgtc tgcatatttt gctgatgtcg acctttgttg ccctgcccgg
     5881 acagatggct gatgcggggt tcccggcggc tgaacactgg aaggtctacc tggcaacgat
     5941 gttaatcgcc tttggctcgg tcgtgccttt cattatctac gctgaagtta agcgcaaaat
     6001 gaagcaagtc tttgtcttct gcgtcgggtt gatcgtggtt gcggaaattg tgttgtggaa
     6061 cgcacaaacg cagttctggc aactggtggt cggcgtgcag cttttctttg tggcgtttaa
     6121 tttgatggaa gcccttctgc cttcacttat cagtaaagag tcgccagcag gttacaaagg
     6181 tacggcgatg ggtgtttact ccaccagcca gtttcttggc gtggcgattg gcggttcgct
     6241 gggtggctgg attgacggca tgtttgacgg tcagggcgta tttctcgctg gcgcaatgct
     6301 ggccgcagtg tggctggcag tcgccagtac catgaaagaa ccgccgtatg tcagcagctt
     6361 gcgcattgaa atcccggaca acgtgatggc agatgaaaaa ctggaaaaaa gcttactgac
     6421 aacgcctggt gtcaaagatg tactgctggc gaagttagag catagcgtct atgtgaaaat
     6481 agacagtaaa atcaccaatc gcttcgaaat agagcgagtt a
//
LOCUS       DAZFRG010000248         6494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFRG010000248 DAZFRG010000000
VERSION     DAZFRG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.249"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(240..728)
                     /locus_tag="ACHKUC_004149"
                     /pseudo
     CDS             complement(240..728)
                     /locus_tag="ACHKUC_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(755..1081)
                     /locus_tag="ACHKUC_004150"
     CDS             complement(755..1081)
                     /locus_tag="ACHKUC_004150"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620306.1"
                     /translation="MTITFRLNVVRDVIDNSLSIREAAVKYGIPAFNTISKWIKKYSE
                     VDFSMKGQKEDLRPKTPAVYQRNRSTQLTISEREELEFLRVENAYLKKLKALAQEKLR
                     LEQKKK"
     gene            complement(1038..1256)
                     /locus_tag="ACHKUC_004151"
     CDS             complement(1038..1256)
                     /locus_tag="ACHKUC_004151"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6620307.1"
                     /translation="MSKFSLEFKREVSEKYLEGELSLKSVGKMYNIDPSTVRKWAYVY
                     RKHGLKTLTGQKGRYDDNIQIECGQRCY"
     gene            1385..2536
                     /locus_tag="ACHKUC_004152"
                     /pseudo
     CDS             1385..2536
                     /locus_tag="ACHKUC_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2999..4174)
                     /gene="senB"
                     /locus_tag="ACHKUC_004153"
     CDS             complement(2999..4174)
                     /gene="senB"
                     /locus_tag="ACHKUC_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFO6620308.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4243..>6494)
                     /locus_tag="ACHKUC_004154"
     CDS             complement(4243..>6494)
                     /locus_tag="ACHKUC_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6620309.1"
                     /translation="KLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKT
                     TSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGSISVNIRGMSGFGR
                     VNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGYEGINAL
                     AGSANMRTIGVDDVIFNGNTYGFRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVM
                     AAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTYE
                     NKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNRS
                     DALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPSG
                     QQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDKEDG
                     FNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQAG
                     FNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSIAEVIGNDWEGIS
                     DEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFGAG
                     DMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLPQ
                     IPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGRT
                     WIFGGDIRF"
BASE COUNT         1642 a         1527 c         1471 g         1854 t
ORIGIN      
        1 cattctagta tagataaaaa aataatggat agcagtgtta gtttattatc atattgtgtt
       61 gtttgttttt cattttttta tctttttagt gtttgtttgt gatttttact cccgattttt
      121 cgtaccaagt agttattaag cacgtaagcg tacagcctga accgtctggt cagaatctga
      181 cgaattagac aaagtggtgt ccaccaaata agtgatctgc gccccaaatc ttggacatcc
      241 tatgaaaaat gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg
      301 cgatggtgat tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta
      361 ttgtatttat ttagatagta acattctgat tttaaagtac taaaaaagct ttccattgct
      421 gcattatcta agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac
      481 caacgttgcc aacgaggcat ctgatattgc catccctgat cactatgcaa caagggctta
      541 tcgtctttat tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat
      601 gggcgctcag taaggttata tgagactatt tctccattat acaggtccat gattggtgat
      661 aaatagagct tcctgccatc gaccttaaac tctgtgacat ctgttaccag taattgtgat
      721 aatggatgac attgccttaa ctcattaata atgattactt tttcttttgt tccaatcgta
      781 atttttcctg agctaaggct ttcagctttt ttagataagc gttctcaact ctcagaaact
      841 caagttcttc ccgttcactg attgtcagtt gtgtcgaacg atttcgttga taaacagcag
      901 gagttttcgg cctcaaatct tccttttgtc ccttcatcga aaaatcaacc tcactgtatt
      961 ttttaatcca tttggatatg gtgttaaaag cagggatccc atacttcact gcagcttccc
     1021 ttatagaaag agaattatca ataacatctc tgaccacatt caatctgaat gttatcgtca
     1081 taacgtcctt tttgcccagt cagtgttttt aatccgtgct tacggtaaac ataagcccat
     1141 ttccttaccg tgctggggtc aatattgtac atttttccaa cagacttcaa actcagctca
     1201 ccttccaggt atttttcgga tacttccctt ttgaattcta atgaaaattt gctcatacgc
     1261 tcctcagtta cagatctggt gtctaactat tggggcgcac ttcataagta gtgggaacca
     1321 atgatcttac ccaccaatag tggacacagg actaagtgag taaactctca accagaggta
     1381 actcatgaca aaaccagtat caatcagcaa gaagccccgt aaccaacata cgcctgaatt
     1441 tcgtaacgaa accctgaaac tcgctgaaca catcggtgtg gccgccgcag cccgtgaact
     1501 cagcctgtat gaatctcagc tttatgccag gcgcagtaaa cagcagcaac aaatgagttc
     1561 gtcagagcgc gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga
     1621 gcaggctgag gaactggcca tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
     1681 atgaagtatg tcttcatcga aaatcatcgg gcagagttca gcatcaaagc gatgtgtcgt
     1741 gtacttcggg ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc
     1801 ctgcgccaac agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca
     1861 aagcagcgat acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc
     1921 attgccgcta gcctgcgtcg tcaggggctg cgggcgaaag cctgccggaa gttcagcccg
     1981 gtcagctacc gtgcacatgg cctgcccgta ttggagaatc tgctggagca ggacttcagc
     2041 gccagtggcc cgaaaccaga agtgggcggg tgacatcacg tacttgcgta ccgatgaggg
     2101 ctggttgtat ctcgcagtag tcatcgacct gaggtcacgc gccgtcattg gctggtcgat
     2161 gtcaccgcga atgacagcac aactggactg tgatgcactg caaatggcgt tgtggcggag
     2221 aagacgcccg gaaagcgtca ttgttcatac ggaccgtggt ggtcaatact gttcagggga
     2281 ttatcaggcg ctgctgaagc aacacaacct gcgtggcagt atgagtgcga aaggctactg
     2341 ttatgacaat gcctgtgtgg aaagcttctt tcattcgctg aaggtggaat gtatccacgg
     2401 ggaacgcttt agcagccagg aaataatgcg ggcaacggtg tttaattata tcgagagtga
     2461 ttacaatcgc tggcgtcgtc acagtgcctg tggcggtctc agcccggaac aatttgaaaa
     2521 ccataatctt gcttagagcc gtgtccacat tacgtgggta ggatcaattt taccgctgct
     2581 ggtccctcat tcagtgactg ccccagggtt tttcatgctc tccttatagt ataataattt
     2641 ccatatccaa actcacccct actttaactt ttatcatagt caaaacctct taacgtgagt
     2701 ttttgttcca ctgagcgtca gccctctaaa actactgaat aatcagttag aaaacaaaca
     2761 atcggaaaaa tctggggttc cggcctagaa ccccatgaac aggcatagct gtgatttcat
     2821 tgaattattg gcacaaatca gttgatcgta caatgctgtg cctgtctcgt tttctggaga
     2881 ttcctcagcc tcatggagcg gcgtaacagg cgtaccggca ggacagagat gtttgctttt
     2941 cctagtccgg ccagtttagt ggaataacgg agtttttatc ccatgttttt tcccccgatc
     3001 acgacttttt caaatcaatg ctcagaatac gttctttagc tccttccaca aacacatcag
     3061 gatcccaatc gcggttaaag ccttctttga tgccaatgta aagggtatcc atatcgtccg
     3121 acaacaccat ctggtttggg taaaccggca tgttaaattc acgggtgatg ctgtatttgg
     3181 tggcatccac tacgctgatg gtgccgctag tgaagttggt gacatacagt tcgttatatt
     3241 tcggattgta tttcaccgcg ttagctccgg ctcctgtgtg cacatagcca agctgtttac
     3301 cgtcgcgtaa gtcaaaaata tagagtccat cttttgtacc ggaacgatcg tcatggtcga
     3361 ataccaccac aaaggcacgg tcagaagcgg agtccaccgc cagattctgc acacttgtga
     3421 tttgtggtct cggatctttc aacgggatgg tacgcagcag cgtctgattt ttcgcatcaa
     3481 tttcgttgat gtaatctctt ccgccaacgt agattttctg tgttttttca tccatcgtca
     3541 gaccatacgc tgtacctttg taagcatcct taacctcgcc ttttaaggca agcgtcgtcc
     3601 cgtcgagcat taacagctta tgcagaggct tcatgccctc ggccgttttc agcatattgc
     3661 tataactcac aaacagggta tcggaatcct ggctgtaaac catatggcgc aaatgctcaa
     3721 aacgcgaatc tgcagcgttt gccgctttaa aacttaacct gtcgctggtt cttaccagtt
     3781 tgccggttgg ggtgtcaaac atactgatac gcagggacgc tgactgggtg tgtccgatat
     3841 agagctgatg tttatcctga tttatcgcca gcgaaaatgc ccggtatggc atcgtgtatt
     3901 tattttcgac tttcagcgaa tctgaattaa acgaatacag atagccttta ttggcttcac
     3961 gatttacacg atcagtgact gctgcaaaca cctttttact ggcattatca aacgccagtt
     4021 catagacgcc ttcatcaggg gactgaaggt aattcgtttt cagggcaaat cgcgaaagtt
     4081 cgcgttcagg tggatcgata gccatggcca tcgtgggtaa aaatagtgaa attaacaggt
     4141 atagcggtgc tttggataaa gtgaaaatat tcatttttcg ttcctgcaat aaaattgaca
     4201 taagagagaa aacgtattgt ctgaacctag cggaaattgt catcagaaac gaatgtcccc
     4261 gccaaatatc catgttctgc cacgcgcgga attggctggg tgggtctcgt caccaagacc
     4321 tggcatcatg ttgagcttat tcagcgcctc cgaataatct ctgttcatca ggttttgtac
     4381 cgaaagtttc agtgtcaggt tgcggttgta ctcgtaagta ccatagagat cgataatcgt
     4441 tgggatctgc ggcaaatcct gtttgattat tgcgccggta tgttcgtcct gctcaaaatc
     4501 aggcgacagg cgacgagcct tgcctgtgta ttttatgata gtgcccaggg tcaacgcgtt
     4561 atcgaagaag cgaacaccag tatccagcgt catgtatttt ctgggcagtt cggtcatatc
     4621 ccctgcgcca aagtaggtgc tggcgatgga ggttggctgg tctgtttgct gctggctgaa
     4681 agagagtcgg ccaaaagcaa aacctgcatc ataatccatc tccagttcga agccctttgc
     4741 gataacgtcg cttgccgagt taacgtagat gtacatattg tcgctgtatt catcgctaat
     4801 gccctcccag tcattgccaa tcacctcagc aatactgcat ttacgacctc cagaacaaac
     4861 cagataagac tcgctgtaga tgtagttctg gatcctgctg cggtacgcca gagccttaaa
     4921 gcgcagggca tcctgttcga ccaataaatc tctggtatca atgttaaaac ccgcctgcca
     4981 ggtttctgca cgttcaggct tcaggaatgg gttcatggat gcgcctcctg aattagagaa
     5041 gaacatctcc tggatgttcg gggcgcgcat ggatttgctg tagccaatga acggctgaag
     5101 ccatggtgtt acctgagcag aaagctgaac tgaagggttg aagccatcct ctttatcgtc
     5161 aatatcgtag ctaccctgtg gcacgcagat aacgcgtgaa tcgcaggcgg gcttgtaccc
     5221 tgtgatcctg ttacgtgtgt agttgagatt gaaatctgcc tcccagatgc cgcgcgtaac
     5281 cttcagcccg gtatacagcg ctgaaatatc ctgctggccg gagggtgcaa acggattgtt
     5341 ctcgatcgat tcctgattcg ctttcgggtc gcccgccgct gagtgaatgg tccggtcata
     5401 gcgggtacgc atcagtttgc tgcccagcat aaactccagt tcattgtccg cgacagtgaa
     5461 ccggctggta ttgttgatat ccagcgcgtc agaacgattt tgcgctgagg ttttgtagaa
     5521 agtatacagc gagccatcac gatacttttg attaccgcga ctggtactgg ccgttacgtt
     5581 aaagtcaatt aattcagaaa aaggggtgta atgatattta atgtaatagt cgtcactggt
     5641 tatatcacga cgtgtaaatt tattttcata ggttcgagcg gaaagttcga agctgttaaa
     5701 ttcgtctggt ctgatatcca ttttatacag ttgggatttg gggttctgct tcatatattt
     5761 atcataacca aactctttgc tgttaattcc agaaccattt gagaaattag agtacacaga
     5821 actgccgctc acagcagcca taacgccaat gcttcccgta tccgtaaaag cgtcgctttt
     5881 tccaccaagg gcgatcattc cgctgcgtcc cagcccatta ctaccgacag agaaacgtga
     5941 acgaaagcca tatgtattac cgttaaatat tacatcgtca acgccaatag tacgcatatt
     6001 tgcactaccg gcaagggcgt tgatcccttc atagccactg ctgtcaccgc gtgtaacatc
     6061 aactgctacc agtaagttag gatctataag tacgccagcc atattgttag ttgaaccatg
     6121 cgtcgttgtt ccggaggtag aggttccgta aaaactctgg gtaataccat cgaccatagt
     6181 gtttacacga ccaaatccgc tcatgcctcg aatattcaca ctgattgatc cctgaccagg
     6241 atctatttga gtataagtac caggcatact acgcactgtt gcgtcaagtg attgcaggtt
     6301 tttgtccgtt gtccgggaac tggtcgctcc tgttttctcg agagcttcct gctcactggt
     6361 cgtcttcccg acagaaacgc tgagcggact gaattctgcc gctccttttt tgtcggaatt
     6421 agttttttca ctgatggatt gtgagtaagc accgcagctg agcaataata tgcctgagcc
     6481 gatagccagt ttta
//
LOCUS       DAZFRG010000249         6484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFRG010000249 DAZFRG010000000
VERSION     DAZFRG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.251"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..257)
                     /locus_tag="ACHKUC_004155"
     CDS             complement(39..257)
                     /locus_tag="ACHKUC_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO6620310.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMIPISPLILVWGIGKII
                     EPVIELYNDVVWAPFNTLHNKINPYKEN"
     gene            complement(259..474)
                     /locus_tag="ACHKUC_004156"
     CDS             complement(259..474)
                     /locus_tag="ACHKUC_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620311.1"
                     /translation="MGIAASYTMHLYCDCRQCTDGKYQSPDFGEYIGTSWAGCAKEAR
                     KDGWRISADKTRAFAPGHKILRSNKGE"
     gene            complement(475..834)
                     /locus_tag="ACHKUC_004157"
     CDS             complement(475..834)
                     /locus_tag="ACHKUC_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620312.1"
                     /translation="MSEINYQEGHEKAGQAKPVAWRYRYVKKGVTDFQGKQWVGDWKY
                     VPTKEDCNDRPNYEIQALFTAPPASVTSEGLVKAVRFYEQVRREDPPVETGAWKDAID
                     WVLKEACLVVNTGIKGG"
     gene            complement(831..1007)
                     /locus_tag="ACHKUC_004158"
     CDS             complement(831..1007)
                     /locus_tag="ACHKUC_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620313.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            complement(1000..1182)
                     /locus_tag="ACHKUC_004159"
     CDS             complement(1000..1182)
                     /locus_tag="ACHKUC_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620314.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIQDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(1278..1634)
                     /locus_tag="ACHKUC_004160"
     CDS             complement(1278..1634)
                     /locus_tag="ACHKUC_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620315.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARICRATKSRTE"
     gene            complement(1612..2073)
                     /locus_tag="ACHKUC_004161"
     CDS             complement(1612..2073)
                     /locus_tag="ACHKUC_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6620316.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            complement(2070..2366)
                     /locus_tag="ACHKUC_004162"
     CDS             complement(2070..2366)
                     /locus_tag="ACHKUC_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6620317.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            complement(2363..2755)
                     /locus_tag="ACHKUC_004163"
     CDS             complement(2363..2755)
                     /locus_tag="ACHKUC_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6620318.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            complement(2771..3541)
                     /locus_tag="ACHKUC_004164"
     CDS             complement(2771..3541)
                     /locus_tag="ACHKUC_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6620319.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNDKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALREVNKHRDIVRQITDSSRRVVSEK"
     gene            complement(3575..4036)
                     /locus_tag="ACHKUC_004165"
     CDS             complement(3575..4036)
                     /locus_tag="ACHKUC_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6620320.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRDESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(4029..5069)
                     /locus_tag="ACHKUC_004166"
     CDS             complement(4029..5069)
                     /locus_tag="ACHKUC_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO6620321.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEEVIDSILQAMQGRVLDGDLITGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKQNEGCHDESRICHDMSRRVTTDKDTDKE
                     LNPTHNAHVRESAPTSESSGTPLQAAEPASLDGLSEPIGKFPMVDDWHPSPDFRRRAA
                     LWGMALPEPEFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(5141..5566)
                     /locus_tag="ACHKUC_004167"
     CDS             complement(5141..5566)
                     /locus_tag="ACHKUC_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6620322.1"
                     /translation="MKIRHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            complement(5550..5825)
                     /locus_tag="ACHKUC_004168"
     CDS             complement(5550..5825)
                     /locus_tag="ACHKUC_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO6620323.1"
                     /translation="MNLRDYLKEKHITQLQFGKLTGLSQVHVSRVLGGYERFSPEKAL
                     RVAEVTNFEVTPHELRPDIYPNPTDGLPVGFKANTPNASELIHENQA"
     gene            5933..6394
                     /locus_tag="ACHKUC_004169"
     CDS             5933..6394
                     /locus_tag="ACHKUC_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620324.1"
                     /translation="MKKTREVIATPEASKNLKAAWNARKKELKLTQELAAELLGFESQ
                     GTVSQYLNGKIPVNTDAALKFAALLKVKPEDIREDLKDLMNYVRSSDTYDDNFSGKGW
                     RLVNEEQAELLNLFEILPASEKAKLLNQLRGLNKLYEEAFENMLALKKRNQ"
BASE COUNT         1491 a         1850 c         1346 g         1797 t
ORIGIN      
        1 tcaaccatgc ctgttctttt tttgtcaaag ttgccatatc agttttcctt atacggatta
       61 attttattgt gcagtgtgtt gaacggagcc cataccacgt cgttatacaa ttcaataact
      121 ggctcaatta tttttccgat tccccatacc agaattaacg gggatatcgg tatcatcaac
      181 acgataaaca gaatgagaaa caaaaattct gtcgctctac tttttcgcgg atattctttt
      241 ctgaataatg taggcacatc actctccttt gttgctcctc aaaattttat gccctggcgc
      301 aaaagcacgc gttttgtcgg cacttattcg ccacccatct ttacgtgcct cttttgcaca
      361 gccagcccat gacgtaccga tatactcacc aaaatctggc gactgatatt tgccatccgt
      421 acactggcgg caatcacaat agagatgcat ggtataactt gcggcaatac ccattcagcc
      481 tcctttgatg cccgtgttta caaccaggca ggcctccttg agtacccagt caatagcgtc
      541 tttccatgct ccggtttcaa ctggcggatc ctcacgccgt acctgttcat aaaagcgcac
      601 agctttaacc agtccttctg atgtcaccga agctggcggg gccgtgaata acgcctgaat
      661 ttcatagttt ggtctgtcgt tacaatcctc ttttgtcggg acatatttcc agtcaccaac
      721 ccactgcttc ccctgaaagt ctgtaacgcc ttttttcacg tagcgatatc gccatgccac
      781 tggttttgcc tgccctgcct tttcatgccc ttcctgataa ttaatctcgc tcattcatcg
      841 ccccactcat cacaatatgc ttcgaccggt gttttccctg cttcataatc atcacgccat
      901 gcttcagcat cagcggcact tccaccgcgt aactctgcat aatccattaa cagttcatgc
      961 cattcttcaa aactggcgtt atatttagtt gaaccaaaat cagccatttt gttcttcctc
     1021 ttcgtctttt atttcgtggt atgagtaatt gcagtagtta aagaaaatat ctttagcttc
     1081 atcctgtatt tcatctggtg ttgcatcatc atccacttcg aattcatcct cgaaatctcc
     1141 accggctatt cccgtttcaa taattatttt gaattttcgc atttcactac cgccctttcg
     1201 gacggcctcc tgatgttctg agggtgcaga aatccctccg gttaaggatt tgattttatt
     1261 tacagtgcta aatttaatta ttcagttctg gattttgtcg ccctgcatat ccgcgctttc
     1321 gcgttacgct caatctgaat taacttttct atatttttcc gcctttcctg ttcctcctgg
     1381 cgcaatagcc ttacatcatc tgccagtctg gtttctcttt tcgccacaga gagcatccag
     1441 tcaaacggct ccacaactgc accgcagatt ttacagcgga cctgacgctc tttttcgtca
     1501 acccgaacag aggcgtgatg acaatatggt ctttccgatg gctcataaag aaaattaacc
     1561 tgattacgag ggtcatcctc ttttaccgga aataaaacga tattgcttaa ctcatcctct
     1621 ggttttattt ccatgctcct ctcctttgat gcgaatgcca gcgacgcgta atgcgtgttc
     1681 taagtcaatc aggtaaagcc aactgccatt ttctttaggt atcatgacat gtcgctcatc
     1741 tgcatttatc gggtgtccat atcgaaggtc gtagcgagtc ggtaattgaa cttcccgcgc
     1801 ttccagttca gcaatacgct tgctcccatc agagataacg ccttcgtaat actcacgctg
     1861 ctcgttgagt tgtgattttg cttcttccag tccatccagc aaatcagcga taatatccgc
     1921 ttcccgatga cggatgtgac gcttaaacgc agcaagagcc gcatcacaat cccgttcagc
     1981 atttgggctg tccgggatag cctgatacca cgccagcgtc gactgatagt tttgtgctgc
     2041 ctcacgaagc gcctcatagt taacctctct cattgagcca cctcctgata aatcaccgca
     2101 tgccccagtt tctccgccag tgccagctct gccttagcgc ccgctgaccg ctgccagcca
     2161 ttcagcatgt aaatcgcatc cacacaacga atcattgcca tgcaaatatc catgtagtgc
     2221 ggctgtgtca gcccgtccgg aagtactgcc gggtttaaga cggtatgccc ttcccgtttc
     2281 agttcctctt ccgccttgtg aaacgcctca cggttgaaat tttcatatcc cgtcattgga
     2341 ccggcaatat aaactctgac cctcactcca tcacctcctg aaagtttccc cgatagaacg
     2401 ccagcacacg ctgcataact tcgctctggc ggcactcacg acaaattatg ttctgccgtc
     2461 tgttgtaacg acgtatttct ccgtcaggta actttcgaat cagtgtcggg tcagcagcct
     2521 tctccggtgt cttacgccat acgcgatacg cctgctctga tggaaatacc ccgcaaccag
     2581 agagccagac atcaccactg gccgcaagcg caccagataa acgacgaata gcggtcttac
     2641 tgacacccgt tttatctgcc agttgtcgaa aagtttctcg tccgctcagg cgcacgaatt
     2701 ccacaatgcg cgccttcact tcttcccgct cttctggtgt aaatactttt gccataagcg
     2761 cctccggcaa tcacttttcc gatacaacac ggcgggaaga atcagtaatc tgtcgaacaa
     2821 tatcccggtg cttgttcacc tcccgcagcg cggcgcagac tcgctcccac ttctgaacat
     2881 cacttttcgc cctgcgcagc gccaggtttg ccctgcgaag ggacggaaaa atcagctcat
     2941 ctgcttgcgt ttcggtaaac gatggcaacg gctgcacaat gtccgccaca gtttctgttt
     3001 taatttcttc ctgtgttgcg gcttcccgga ctggtaacgc agcacctgct ggctgaggaa
     3061 aggccttacc atcactttcc gttaccagcg cggctttcgg ctctgctggt aaattatcgc
     3121 ccggcatgca gtaacgaaat ttatcgttct gattaacgcg tgccagccgc cccgttgcgg
     3181 ttaccaccgc cagcgtggag gcaaccttgc gagtactgac gccgaactta cccgccagtt
     3241 cctcacacgt tttagcacca tcctgaccga taaactcaat catcatgtct gcggtaactt
     3301 tttgttcgac ctccccggtc agcatatcct gtgcttcaga ttttactggc cgctcttcgg
     3361 ttacccggga ttcaccttcg ccagccagaa accaggtgtg accagtttta tcaacgacgc
     3421 catttctttt gagttcccac agctcgttga gaacctcttc acgactgata tcaagtcgcg
     3481 cggccagttc taccgatgtg gcttttccca ttgctttcag tgcgtcaaat acggtttcca
     3541 ttaaaatttc ctccgacaaa atcgtttctc agattcaaat aaaaccagct gccttccggc
     3601 gttcgtattc ctgtttcagc cgttcaattg gcgttggccc ttgcgggtgt ttcgcccctt
     3661 ccagttgtcg tcgcactggc ggaacactca tcccgttacc aacatgcttt gcccatttcg
     3721 tcagttgccg ttccgcaagt cgttttaact caccctgcgt catctggcgc tcaatccctc
     3781 tggtacgcat ttcgaggcag atgtggtaca gcacaggctg aggccacgga tatttgtcgc
     3841 ttccgtcata tcgccaggac tcatcacgcc agcggcggta ctcctccatc acagcatcca
     3901 ccgtcaggcc aaatggattg gccccgcttt ctgaaatcag cgccacaaac tcagccaggt
     3961 ccggaggcca tgtttcaccc gcccggcagc ggtccatgca ctggcggcag acctgtcgga
     4021 tttgctgctc agtcatcgcg ccaatctgtg caatccagag cttcgaaggt gcggccccgt
     4081 tcttctgggt ccagcggttc gaataaacct cccccatgag ttcccacagc ttccagaccg
     4141 tttccgtcgc tgataaatcc gttttcacgt tcccactgct cacgtgctgc ccgaatttcc
     4201 tgaactgccc gtgatgcggt gccacctggt gctgctgcat ggtttacccc cttgctgact
     4261 ggcttaacct gcgccctgac gtgatttacg tgacgggcga atttctgctc ccactgaacc
     4321 tgcgtgaaaa ctttcccctc cgctgcccag tagtcccgga aagcggcaag ttcagcaggt
     4381 gtaaattccg gctccggcaa agccatcccc cacaacgcag cccgtcgtcg aaaatccggc
     4441 gacggatgcc agtcatcgac catcggaaat ttcccgatgg gttcgctcag tccatccagg
     4501 gatgcaggtt ctgctgcctg caacggcgta ccactcgact cactggtcgg agcactctcg
     4561 cgcacgtgcg cgttatgtgt ggggtttaat tctttatctg tatctttatc tgtcgtgact
     4621 cgtcgtgaca tgtcgtgaca tatgcgtgac tcgtcgtgac acccctcatt ctgttttcgt
     4681 aatttttccc tctcgcgctg cgctctcttg cgctctgccg gggatttcgc ggtttgtgaa
     4741 acgttgccat tgtcctcttt cagtacctgg cgtttttccc atccggtgat taaatctcca
     4801 tcaagtaccc gcccctgcat tgcctgtaaa attgaatcaa ttacttcttc cgtcacatca
     4861 agcgcacttg ctaaatcttc cgtcgtgaca tcaatgtgac cacgtagtga cacgccgtga
     4921 catgtcgtga catttcgtga cgcgctcacc agaaggtgga tatacactgc catcactgtt
     4981 gcaattggct gccctgacac cctggcaatt gttcgccact tagggtcatt tggcatgtca
     5041 tgccataatc tgagccaggc gttagccata ctcacctctt ctgataccga atctttttac
     5101 tcacaaattg ccggaagtga tccggtatga atattgcgag tcaatgcaca gccacaatat
     5161 ttcctgcagg gccaccacga ttcatctggt tgaaaccagc gatcgccact gcgacaaaat
     5221 catcagcgtc tctcaccagt cgttcccgcg tctccaccag ctcccgaaaa taagctgaac
     5281 tgtggctgcg cattcgggcc accagcagag gcggcattgc tttttcgatc gctggtaaca
     5341 acgcctgaat ttttttaacc gcatcaggag tgtctttctc cacccagcgg aaaattttct
     5401 gggtattgcg agccagggct tccggatggc tgtcgtcata taattccggg aacgtcatac
     5461 caagctcaaa ataagcccgg gttatttcag ctgccggaac tttttcaccg tccggatgcg
     5521 cccaggcatt catcgccatg cggatgtgct catgcctgat tttcatgaat caactccgat
     5581 gcatttggtg tgttagcctt gaatccaaca ggtaagccgt cggttggatt cgggtaaata
     5641 tcaggccgga gttcatgagg tgtaacctcg aaattcgtta cttcagcaac acgtaatgct
     5701 ttttcagggc tgaatctttc atagcccccc agcactcgac ttacatgcac ctgagataaa
     5761 cccgttagct tcccaaactg tagctgggtg atatgtttct cttttaaata gtctcttaag
     5821 ttcatagcca accttctacg ttatgtctcg agcaaatatt agccccgcta attttaaaga
     5881 tcaatagcca gactatcttt gataatattg gtaaaacaaa taaactctat gtatgaaaaa
     5941 aacacgcgaa gtgattgcaa ctccagaagc gagcaagaat ttaaaagccg catggaatgc
     6001 aagaaaaaaa gagctgaagc tgactcaaga gctggcggct gagttgttgg gattcgaatc
     6061 tcaaggcacc gttagccagt atctgaacgg caagatacca gtaaataccg acgctgcgct
     6121 aaaatttgcg gctctgttaa aggtaaaacc agaggacatt cgagaagacc ttaaagactt
     6181 aatgaattat gtaagatcat cagatactta tgatgataac ttttcaggca aaggatggag
     6241 gctggtcaat gaagaacagg cagagttact taacctcttc gagattctac ctgcgtcaga
     6301 aaaagccaaa ctccttaacc agctacgtgg actaaacaag ctctacgagg aagccttcga
     6361 gaacatgctg gcactaaaga aacgtaacca gtagccaccg ctcactaccc catccacaac
     6421 aaaaaaaccg acgtcttagt cggttttttt gtgccataac ttctgaaaat cagctgtata
     6481 acta
//
LOCUS       DAZFRG010000250         6441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFRG010000250 DAZFRG010000000
VERSION     DAZFRG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.252"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..79)
                     /locus_tag="ACHKUC_004170"
     CDS             complement(<1..79)
                     /locus_tag="ACHKUC_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620325.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLD"
     gene            complement(102..320)
                     /gene="ydfC"
                     /locus_tag="ACHKUC_004171"
     CDS             complement(102..320)
                     /gene="ydfC"
                     /locus_tag="ACHKUC_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6620326.1"
                     /translation="MQKREPVIIVPDYTDDELYEWIHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(350..478)
                     /gene="ydfB"
                     /locus_tag="ACHKUC_004172"
     CDS             complement(350..478)
                     /gene="ydfB"
                     /locus_tag="ACHKUC_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6620327.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(480..635)
                     /gene="ydfA"
                     /locus_tag="ACHKUC_004173"
     CDS             complement(480..635)
                     /gene="ydfA"
                     /locus_tag="ACHKUC_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6620328.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(908..1624)
                     /locus_tag="ACHKUC_004174"
     CDS             complement(908..1624)
                     /locus_tag="ACHKUC_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620329.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDCHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGINNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1674..1889
                     /locus_tag="ACHKUC_004175"
     CDS             1674..1889
                     /locus_tag="ACHKUC_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6620330.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            1886..2311
                     /locus_tag="ACHKUC_004176"
     CDS             1886..2311
                     /locus_tag="ACHKUC_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6620331.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            2334..3296
                     /locus_tag="ACHKUC_004177"
     CDS             2334..3296
                     /locus_tag="ACHKUC_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620332.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDLSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            3303..4049
                     /locus_tag="ACHKUC_004178"
     CDS             3303..4049
                     /locus_tag="ACHKUC_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6620333.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTRKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            4071..4787
                     /locus_tag="ACHKUC_004179"
     CDS             4071..4787
                     /locus_tag="ACHKUC_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6620334.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQEMM
                     AQEEMKTEIVEDIVRLQPSITEMKADDLILPSLHVANRELRRAKSDVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            4784..5101
                     /locus_tag="ACHKUC_004180"
     CDS             4784..5101
                     /locus_tag="ACHKUC_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6620335.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMILRGDFHPSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            5098..5400
                     /locus_tag="ACHKUC_004181"
     CDS             5098..5400
                     /locus_tag="ACHKUC_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6620336.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEDALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGARAELALAEKLGHAVIFQEVGSEY"
     gene            5468..5670
                     /locus_tag="ACHKUC_004182"
                     /pseudo
     CDS             5468..5670
                     /locus_tag="ACHKUC_004182"
                     /inference="COORDINATES: protein motif:HMM:NF027274.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            5657..6091
                     /locus_tag="ACHKUC_004183"
                     /pseudo
     CDS             5657..6091
                     /locus_tag="ACHKUC_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            6258..>6441
                     /locus_tag="ACHKUC_004184"
     CDS             6258..>6441
                     /locus_tag="ACHKUC_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620337.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVV"
BASE COUNT         1702 a         1399 c         1786 g         1554 t
ORIGIN      
        1 ggtcaagagt cgccagttga gagcgaacca cattcgctgc catttcctgg aatggcattg
       61 gtaaatcttt aaattccatt attagcctcg ttggttagct attaacgcgg gtatgtaatc
      121 attctggcaa tgcttaatgc cgctgctttt tccagcctgg tgatatcctg ctccagagcg
      181 gacagatttt cagcctgctt agccctggct tcattggccc atttcaggtc ctgcgcagcc
      241 ttaattttct ggtgtatcca ctcataaagt tcatcatcgg tatagtctgg cacgatgatg
      301 acgggttctc gtttctgcat actgattcct cgcggtgctg tttcgcttat cagccgttag
      361 attttgccga actggaaagc gcctgtttaa actcactgaa gctgagggct tcttcgcctt
      421 cggcaaggcc ttcgaagtat tcttcgtaag ccttttccat gattgtgttg aaatccatat
      481 cactcacctg agtttctttc cagccagcga cgggcaccat tttcggtttt aaacgttttg
      541 cttttggtat acgtcatcgc ggtgaacgta ccgtcctggt tggggaacac gccacatacc
      601 agagattcgc tgttgccaag atcgatagta tccatgctga cctcatttcc ccttaacgcc
      661 agggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc
      721 ttacgcctcg ggctggctac ttaacccctg accactgccg ggtaactcga agtatttccc
      781 tgcgttctgt ggggcggggt gggttggtat gttgttaagg taacaagagt tacctttcga
      841 gtcaatacaa tgttgcaaaa ggtacatttg agggcataaa aaacccgcaa tgaatgcggg
      901 ttctgactca gtctaagtat tgatgtattt gtgaaacttt acctttaatg gtgtaaccac
      961 cattcagttc gatgggtttg taaagcggat tcagtgacaa caaatagatg tttggtccgt
     1021 caatcgcaac tttttttagt gttacgtttg gcgttccttc caattggatt aagattattt
     1081 ttcccaccag ttctctaatg ttacttgagc atggtgtgat cagcacggta gatccgtcgg
     1141 ggatggttgg gaggccgtta gagtttgtca tcgcatctcc ctcaacatgc aataaaaaag
     1201 agttttcagc ggtttttgtc atgacatcaa cccagttatt aataccagga atcttggtta
     1261 ctggacaact catatcccaa taaccagcct gttcccacgt taaaacgggc aaccgggcga
     1321 tgttgtcact aatgtaaggg tactgattca gacgcagatc atcggtttta tcgtgacagt
     1381 cctttccata aagaatccat tcaggagatt tggaaagcaa ttttgacagt agatacaaat
     1441 tctcaccgtc aggttttgaa gagccatttt cccattttgt tacggataca cgagatatgc
     1501 cgattgcttt cgcaacctgc tgttgggtta atccaacgtc ttttcgacga ttccgaatac
     1561 gttcgctgat agtgtttttc atgtaaccaa tgttactacc aagtgatgtt gctatggttg
     1621 acattgttat gtaactattg ttacccttct gctcgaaata acaggagagt tttatgttca
     1681 aagatgatgt tctgcgctat ttcaaaaaaa agcgactagt agctgaggct cttggaattt
     1741 cacatgtggc tgttgtgcgg tggaaagcag ttattcccaa acttcgcgca atggaactgg
     1801 atgaaattac taacggtgaa ttgaaataca acccagaact ttacaagaag caggatagca
     1861 cctcgaacga aggaaagaat gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     1921 gaatgtctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     1981 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccctggc
     2041 ccgtaatacc cagaaaattt tccgttggct ggataaagac acccctgatg ctgttgaaaa
     2101 aatgcaggct ctgttaccgg cgatcgaaaa ggcgatgccg cctttgctgg tggcccgtat
     2161 gcgcagccac agttctgaat attaccgtga gatcgtcgaa cggagggatc ggctggtgaa
     2221 ggatgtcgat gattttgttg cgtcagcggt tgttttgtat gaccagatga atcgcggcgg
     2281 cccggcaggg aatgctgtgg tgatgcacta aaagcacggt gttcgggggt tttatgagca
     2341 gcaagcttca tggtcttgtc tgggaagggt gcgccttcac cggcatgatc ttatccaggg
     2401 tggcggttat ggcccgtctt gcagactaca gcaatgacga gggcgtgtca tggcctgcca
     2461 ttgaaactat ccggcgtcag atcggtgcaa gaagtgaatc cacagtgaaa tcggctattg
     2521 cagaactggc gaaagagggc tggctgacga aggaagagcg taaggtcggt gggcgtaatg
     2581 taagcaatat ctatcggctt aatgtggaaa aactcgaagc agctgcggcg gcggcgcgtg
     2641 agtcatataa accgaaaaga aaaattagcc cggcaaaaaa tgacccgtta acagttgacc
     2701 cgtcaaatat tgacccctca acggttgacc tgtcaaattt tgatggatca actgttgata
     2761 aaaaactgcc gattaggggg gcgatgattg accccgatcc gtcagtatta aaacctgatc
     2821 cgtcagataa aagatcttct tgtccggacg cttcgcaacc ggacccgcag acggctgaac
     2881 aggatttttt aacccgacac cctgacgcgg ttgtgttcag tgcgaaaaaa cgccagtggg
     2941 gaagtcagga agatttggtg tgcgcacagt ggatctgggg acgaatcgtg agtctttacg
     3001 agcaggcggc cagcgatgat ggcgagatca ctagaccgaa agaacccaac tggacagcat
     3061 gggccaatga cgttcgcaca atgcggatgc tggatggcag aactcacaga caaatttgtg
     3121 aaatgtttgg gcgtctccag cgggattcgt tctgggtaaa aaacatcatg agtccggcaa
     3181 aactccggga aaaatgggat gaactggtta tccgcctggg gcgttcgcct gcgcagcgtt
     3241 gcgtgaatca catttctgaa ccggacactg aaataccgcc gggattcagg gggtgacgtg
     3301 tcatgaaaaa cattgcggca gttggggttc ttgaacgtat tcgcagactt gcaccacagg
     3361 ggtcggttcc accgtaccgg acggtggagg agtggcggga atggcaactt gctgaaggac
     3421 gaaaacgcag cgaggagatt aaccgccaga atcgccagtt gcgggtggaa aaaatcctga
     3481 atcgttcggg catccagcct ctgcacagca aatgctcgtt tgcaaattat caggtgcaga
     3541 acgacgggca aaaatacgcg ctgagccagg ccaaatccat agctgacgaa ctgatgaccg
     3601 ggtgcacgaa ttttgtgttc agcggtaaaa ccggcaccag gaaaaatcac cttgcagcgg
     3661 cgatgggcaa ccggctgatg gtgaaggggc gcagcgtgat tatcgtcacc gtgtctgacg
     3721 tcatgagcgt gttgcatgac agctacgaca acggcaaatc cggggaaaaa tttttacagg
     3781 agctttgcgg ggttgatttg ctggtcctgg atgaaatagg cgttcagcgg gagacgaaaa
     3841 acgagcaggt ggtattgcac cagataattg atcgccggac agcatcactg tgcagtgtcg
     3901 ggatgttaac aaacctgaat catgccgcaa tgagtacgct tcttggtgag aggattatgg
     3961 accgcatgac catgaacggt ggtcgatggg tgacgtttaa ctgggatagc tggcgtccaa
     4021 atgtcagcaa tatgagggtt gtgaagtaat tttgtccgga ggaaatatta atggaaaccg
     4081 tatttgacgc actgaaagca ctgaaaagag cctcttcaca ggtagtggcg gcccgccttg
     4141 gaatcagccg tgaagatgcg gtcaacgaac tgtggaaact gaagcgccgc ggtgaagcgg
     4201 ataacaaggg ttcgatgtgg tggctgattc aggctggtga aagtgaaccg gtgtcaccgg
     4261 taccgaaagt gacagcgcaa atgctgactg aggcgattga acaacatggc ccacaaacgg
     4321 cggatgagct ggcactgatg ttcgggatta cctcgcgccg ggcgaattca tcgctggcca
     4381 tggcaatcag caaagggcgt ctgattcgcg tgaatcaggg cggtaaattt cgttactgca
     4441 taccgggcgc tgatttaccg gcagagccgg aagctgcatc cgtagcggaa accgatggta
     4501 aagcctttcc tcagccagca ggtgttgcgt taccagtaca ggaaatgatg gcacaggaag
     4561 aaatgaaaac agaaatcgtg gaagacattg tgaggttaca gccatcgatc accgaaatga
     4621 aagcagatga cctgattcta ccatcgctgc atgtggctaa ccgcgagctg cgccgggcga
     4681 aaagtgatgt ccagaagtgg gagcgagtct gtgctgcgct gcgggaactg aacaaacaca
     4741 gggatattct ccgggatatt accgccacca gagagcagca gcggtgagtg gctggaagaa
     4801 gtggcgctgg gctgaaatcc tgatactccg gcagtgtgcg ggaacgatga gagtcgaaag
     4861 catcggttat ctgattggtc gtagtgagtc agccgtcagg acgaaagcgc gggaactggg
     4921 tatcagcatg attttacggg gtgattttca cccgtcggca aaatattcac agcgtgatat
     4981 tgagctggcg cggcaactgc atcagcgtgg cgtaccccga cgggaaattg ccgaaaagtt
     5041 tgggatgaag ttgggcgcag tgaataacta cgtttatttc gacaggaggg ttcaggagtg
     5101 agggtgagga tttatatcgc cggtccgatg acgggatatg aaaatttcaa ccgcgaggcg
     5161 tttcacaggg cggaagatgc gctgaaacgg gaagggcata ccgttttaaa cccggcagta
     5221 cttccggacg ggctgactca accacactac atggatattt gcatggcaat gctccgttgc
     5281 gtggatgcgg tttacatgct gaaaggctgg cagcagtcgg caggtgcaag ggctgagctg
     5341 gcactggcgg agaaactggg ccatgcggtg atttttcagg aggtgggcag tgaatattga
     5401 cccggcgata acgattgata tggccctgaa caccggcctg gcacttcttg gttatttcta
     5461 catcatgttc tgcagcggac gatggctgtc actgttgttc aaaaatggaa taaacgccgt
     5521 aagcaggagc aacgccagaa ggcaatggat gcatttttcg aagccttcgg gattgacagc
     5581 atggaaccag gggatccagc tcgcgtaatt agcagaggtg acgttgtaat tcttgtatac
     5641 cggaatgaag agaaaaatga gcgagattaa ctatcaggta ctgcgtgaaa aggcagagaa
     5701 agcaactaaa ggaagctaca tcgtagggca tacatctgtt aaccaacacg gcaatttaac
     5761 aggagttttt gtttgtcaaa aatggaaagg agaacccggt ggcgtgattg cggaacgtca
     5821 tgttaactgc ctgattgaaa cagatgctca ggcttatgca aacgctgagt tcatagcaga
     5881 ggctaacccg gctaccgtgc tggcactgct ggatgaacag gaaagaaacc agcaatacat
     5941 caaacgccgc gaccaggaga acgaggatat tgcgcttacg gttgggaagc tgctcgttga
     6001 gcttgaagca gcaaaatcaa aactcaacga gcagcgcgag tattacgagg gagttatctc
     6061 tgatgggtgc aagcgtattg ctgaactgga agcgcgggaa gttcaattac cgactcgcta
     6121 cgaccttcga tatggacacc cgataaatgc agatgagcga catgtcatga tacctaaaga
     6181 aaatggcagt tggctttacc tgattgacct ggaacacgca ttacgcgtcg ctggcattcg
     6241 catcaaagga gaggagcatg gaaataaaac cagaggatga gttaagcaat atcgttttat
     6301 ttccggtaaa agaggatgac cctcgtaatc aggttaattt tctttatgag ccatcggaaa
     6361 gaccatattg tcatcacgcc tctgtccggg ttgacgaaaa agagcgtcag gtccgctgta
     6421 aaatctgcgg tgcagttgtg g
//
LOCUS       DAZFRG010000251         6414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFRG010000251 DAZFRG010000000
VERSION     DAZFRG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.253"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..330)
                     /locus_tag="ACHKUC_004185"
     CDS             complement(<1..330)
                     /locus_tag="ACHKUC_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO6620338.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
     gene            482..811
                     /locus_tag="ACHKUC_004186"
     CDS             482..811
                     /locus_tag="ACHKUC_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO6620339.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(868..1611)
                     /locus_tag="ACHKUC_004187"
                     /pseudo
     CDS             complement(868..1611)
                     /locus_tag="ACHKUC_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            1701..2132
                     /locus_tag="ACHKUC_004188"
     CDS             1701..2132
                     /locus_tag="ACHKUC_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6620340.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            2341..2586
                     /gene="nrdH"
                     /locus_tag="ACHKUC_004189"
     CDS             2341..2586
                     /gene="nrdH"
                     /locus_tag="ACHKUC_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO6620341.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2583..2993
                     /gene="nrdI"
                     /locus_tag="ACHKUC_004190"
     CDS             2583..2993
                     /gene="nrdI"
                     /locus_tag="ACHKUC_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO6620342.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2966..5110
                     /gene="nrdE"
                     /locus_tag="ACHKUC_004191"
     CDS             2966..5110
                     /gene="nrdE"
                     /locus_tag="ACHKUC_004191"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6620343.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTCYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYSKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            5120..6079
                     /gene="nrdF"
                     /locus_tag="ACHKUC_004192"
     CDS             5120..6079
                     /gene="nrdF"
                     /locus_tag="ACHKUC_004192"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6620344.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         1630 a         1625 c         1574 g         1585 t
ORIGIN      
        1 aaataaacca ttcaaataac gttcaagcgc aacacgcgaa ctaaagccat gcgggatacc
       61 gtaatgctca tgacgttcac ccgctaaata gagcggaaat tgctggtaat gatcgcaagt
      121 tttaatatcg gaagcgggtt cagccagtgt tgaactacgt tcttttaatg ttggatgaat
      181 caccagggcg gtacgtccca ttcgcgcttc gcgattaaca tagacataat tttccccacg
      241 gcggtaacca tacgctttct gggttaccac atcgacagta aaaccgactt tttcaagtac
      301 gcgcgccacc tcgtctggtc gtaaatacat atttgatcct cgttattatc aaccgcgggc
      361 ctaccttacc tgattgcgca ttcacaacgc tttcagaaaa gtccataaag ccgcgatgaa
      421 cctcagttaa gaaaattatg gtctacactg aaaattacat cgaattctaa tgggggatca
      481 tatgtttaac cgaccgaacc gcaacgacgt tgatgatggc gtgcaggata tccagaatga
      541 tgtcaatcaa ttagctgaca gtctcgaatc tgtattgaag tcctggggca gcgacgccaa
      601 aggggaagcc gaagccgctc gtagtaaagc gcaggctcta ctgaaagaaa ccagggcgcg
      661 aatgcatggt cgtactcgcg tccagcaagc cgcgcgcgat gccgttggct gcgcggattc
      721 ttttgttcgt gaaagaccct ggtgtagtgt gggtacagcg gcagcggtgg gtatttttat
      781 cggcgcactg ttaagcatgc gcaaatcgta gtgcaaaaat gataataaat acgcgtcttt
      841 gaccccgaag cctggcttcg gggtttttta gcttaaggct ttacgtaatt gttgtgctac
      901 ctgtcgcgcc atagcttcgt taacgatatt agtaaacgaa agcagtaatc caccttcccc
      961 acttgaacgg atacgccagt cgctaagtgc ctgcaccgca aggcctgcag cattggcttt
     1021 acgcgcatgg gcaatatcat cgcctatcat tctgatcacc atctggatac cacctttctg
     1081 cggcacaacc tgaaatcctt gctgcgtcaa cgcttgctca atccattgcc gacgttgagc
     1141 ataatgctga cgcattttct tcagatgccg ccagaaatgc ccctcgcgaa gaaagtctgc
     1201 cagtgtgttc tgccatagaa caggtaccgc acatggagcc agtgacgcct ggtggcggaa
     1261 ttgtgtaatt tgcttcaccg gcaccaccag ccacgcacag cgcaatgcag gaaatagcgc
     1321 tttgctgaat gttccggcat aaattacccg ctgcggtgcg tcgagacttt ttaacgccgg
     1381 taacggctta ccgtgatagc gaaactcact gtcataatca tcttcaataa tccatgcctg
     1441 actacgatct gcccattcca gtatctgatg cctgcgcgct aaagagagcg ccacacccag
     1501 cggactttgg tgtgctggtg ttatcagggc aaaacgcgca tcaggataat tttgtattcc
     1561 gcttgtgata tccagtccat tgtcatcaac cggcacaggc aaaatttcca catctggtct
     1621 gcttttttag gtaatttatg gctctcttta acagtccatt atagtgctac attgcaggcg
     1681 ttaataagag gagaaactgt atgactacat tacgtcagcc ttactacgaa cttagcccgg
     1741 cagtgtataa cgcactagtg caggccaaaa cggcactgga aaatagcacg ctggatacca
     1801 cgttgatgga gttagtttat ttgcgcgtct cgcaaatcaa cggctgcgca ttttgtctgg
     1861 agatgcacag caaagcattg cgcaaatccg gcgtgccaca gcacaaactg gacgccctgg
     1921 caggttggcg cgtaagccat cattttgatg aacgcgagcg ggcggcgctg gcatgggcag
     1981 aatcggtaac cgaaattgcc agaacccatg cggaagacga ggtttatcag cctttgcttg
     2041 agcatttcag cgcagcagag atcagcgact taacgtttgc catcgggctg atgaattgtt
     2101 ttaaccgtct ggccgtttcc atgcggatgt aacttcttga tgccgggtat ttgcccggca
     2161 aaaaaacaca aaatccccta ccccgttgag ccattcatcg gtggcgtaat caatctaaaa
     2221 ttcccatatc ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
     2281 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc
     2341 atgcgcatta ctatttacac tcgtaacgat tgcgtccagt gccacgccac caaacgggcg
     2401 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcggca
     2461 gaagcgctgc gtgctcaggg ctttcgtcag ttgccggttg tgattgcagg cgatcttagc
     2521 tggtctggtt tccgcccgga catgatcaat cgtctgcatc cagcgccaca cgcggccagt
     2581 gcatgagcca gctcgtctac ttttccagca gctccgaaaa tacgcagcgt tttatcgaac
     2641 gtttagggct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     2701 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     2761 gacaggtcat tcgcttttta aacgacgagc ataaccgggc gttgctgcgc ggcgttattg
     2821 cttcgggtaa tcgcaacttt ggtgaggcat atggccgcgc cggagatgtg attgcccgga
     2881 aatgcggcgt gccgtggctg taccgctttg aactcatggg tacgcaaagc gatatcgaaa
     2941 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaacgcc tgacgcagga
     3001 aacgatggat tatcacgctc tgaatgcgat gcttaacctt tacgatagcg caggtcgcat
     3061 tcagttcgat aaagacagcc aggcagtaga cgcctttatg acgacgcatg tgcgtccgaa
     3121 cagtgtggcc ttcagtagcc agcagcagcg cctgaactgg ctggttaacg aaggttacta
     3181 tgatgaaagt gtccttaatc gctactctcg cgattttgtc attacgctgt ttgcccacgc
     3241 gcacgccagc ggttttcgtt tccagacatt cctcggagca tggaagtttt acacctgcta
     3301 tacgttgaag acattcgacg gtaagcgtta tctggaagat tttgccgatc gcgtcacgat
     3361 ggtggcgctg acgctggcac aaggcgatga aacactggcg acgcagctca ctgatgagat
     3421 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     3481 cggcgaactg gtttcctgtt ttctgctgcg tattgaagac aatatggagt cgattggccg
     3541 ggcagtaaat tccgcactac aactgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     3601 gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtgat
     3661 tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     3721 gggggctggg gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgatac
     3781 aaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     3841 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     3901 tgacgtagag cgagtttata gcaagccgtt tgccgatgtc gccatcagtg aacactatga
     3961 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     4021 gcgactggca gaaatccagt ttgagtccgg ctatccgtac atcatgtatg aagacacggt
     4081 taaccgtgct aaccctatcg ccgggcgcat aaatatgagc aatctctgct cagaaatttt
     4141 gcaggttaac agcgcctcag aatatgacga gaatctcgac tatgctcgta caggacatga
     4201 tatttcctgc aatctagggt cgttgaatat tgcccacacc atggattccc ccgactttgc
     4261 ccgcacggta gagactgccg tacgcggttt aacggcggtg tcagatatga gtcatatccg
     4321 cagcgtgccg tccatcgaag ccggaaatgc cgcctcacac gccatcggtc tggggcagat
     4381 gaatttacac ggttatctgg cacgagaagg catcgcttat gggtcgccgg aagcactgga
     4441 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     4501 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     4561 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     4621 gtttgcccgt agcggtatta cgttacctac ccgtgagatg tgggcacagc tgcgcgacga
     4681 cgtgatgcgc tatggtatct acaaccagaa tctccaggcg gtgccgccaa ccggttctat
     4741 ctcttatatc aaccatgcca cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     4801 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     4861 ggcgctgtat caggacgctt acgaaatcgg cgcagaaaag atcatcgaca cctacgcgga
     4921 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     4981 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aagggtatca aaacgctcta
     5041 ttacattcgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     5101 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     5161 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     5221 aaaggtgcca ctgtcgaacg atattcctgc atggcagaca ttaagtgtcg tagaacaaca
     5281 actaacaatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     5341 cgcgccctcg ctgatgcccg atgcactcac cccccatgaa gaggcggtat tatcaaatat
     5401 cagctttatg gaagcggttc atgcccgctc ttacagttca attttctcga cgctgtgcca
     5461 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     5521 agctcagatt attcagcaac attatcgcgg ggatgatccg ctgaaaaaga aaatcgccag
     5581 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     5641 ccgcggaaag ctaaccaata ccgcagacct gatccgtctg attatccggg atgaagcagt
     5701 ccacggttat tacatcggct ataaatatca gaaaaacatg gaaaagatct cccaggcaca
     5761 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactttatg acaacgagtt
     5821 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     5881 ctgttacaac gccaataagg cgttgatgaa tctgggctac gaaccgttat ttcccgcaga
     5941 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     6001 cgatttcttt tccggttcag gctcttcata tgtgatggga aaagcggtcg aaacagaaga
     6061 tgaagactgg aatttctgag ggtgttattt ttcaaaaata tcactacccg cagcagggaa
     6121 ataattcccg ccaaatagct ttttatcacg caaatatttt gtggtgatct acactgacac
     6181 tctgttgcat tattcgcctg aaaccacaat attcaggcgt tttttcgcta tctttgacaa
     6241 aaaatatcaa cttttctcga tttgctctca gcccttatat catgggaaat tccggcgatt
     6301 tgctcacatc aatattcatg ccacatttgc catcaggggt tgcctcagat tctcagtatg
     6361 ttagggtaga aaaaagtgac tatttccatt gggtaatgta tcgacataaa caaa
//
LOCUS       DAZFRG010000252         6393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFRG010000252 DAZFRG010000000
VERSION     DAZFRG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.254"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..146
                     /locus_tag="ACHKUC_004193"
     CDS             <1..146
                     /locus_tag="ACHKUC_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO6620345.1"
                     /translation="DFARAQREAIIKKVTEVLRPKYGHMYGYDASMPANIEMAIRLVG
                     SYY"
     gene            complement(289..1653)
                     /gene="ygbN"
                     /locus_tag="ACHKUC_004194"
     CDS             complement(289..1653)
                     /gene="ygbN"
                     /locus_tag="ACHKUC_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO6620346.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(1742..2518)
                     /gene="ygbM"
                     /locus_tag="ACHKUC_004195"
     CDS             complement(1742..2518)
                     /gene="ygbM"
                     /locus_tag="ACHKUC_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO6620347.1"
                     /translation="MPRFAANLSMMFIEVPFIERFAAARKAGFDAVEFLFPYDYSTQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHAQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(2523..3161)
                     /gene="ygbL"
                     /locus_tag="ACHKUC_004196"
     CDS             complement(2523..3161)
                     /gene="ygbL"
                     /locus_tag="ACHKUC_004196"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO6620348.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(3158..4420)
                     /gene="otnK"
                     /locus_tag="ACHKUC_004197"
     CDS             complement(3158..4420)
                     /gene="otnK"
                     /locus_tag="ACHKUC_004197"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO6620349.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVES
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTEILAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(4417..5325)
                     /gene="ltnD"
                     /locus_tag="ACHKUC_004198"
     CDS             complement(4417..5325)
                     /gene="ltnD"
                     /locus_tag="ACHKUC_004198"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO6620350.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            5521..6288
                     /gene="ygbI"
                     /locus_tag="ACHKUC_004199"
     CDS             5521..6288
                     /gene="ygbI"
                     /locus_tag="ACHKUC_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO6620351.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
BASE COUNT         1612 a         1674 c         1626 g         1481 t
ORIGIN      
        1 ctgattttgc gcgagcccag cgcgaagcaa ttattaagaa agtaactgaa gtattacgtc
       61 cgaaatatgg ccatatgtat ggctacgacg catctatgcc tgctaatatc gaaatggcaa
      121 taagattggt cggttcttat tactaagata aaatcaactt gcaagagatt gtaattatat
      181 tgcaaagtga aaattaacct cggtagcgag atattaccga ggttaatatt ggcatacatt
      241 acccccttat caggcactta aacgatacat catatcttat ctggattatt agatgacgag
      301 ccatacgcac caggtaatta agaatccggt aaaaccgaga atggtcgtta acactgtcca
      361 ggttttcaga ccgtctgcta ccgacaaccc cagatatttg gtcacaatcc agaaccctga
      421 gtcattaata tgcgacgcgc caagcccacc aaagcaggct gccagcgtca ccaatacgca
      481 ctgaatcgga tttaatccca tcaccgcttc tgagagtaac ccgccggttg tcaggattgc
      541 tacggttgct gacccctgcg atgcacgcag cgccagtgaa ataataaatg cggctggtaa
      601 cagaggcagg tcaatcattt gtaacatgtt ggcaagggct ttaccgacgc ccgattccac
      661 cagcacttta ccaaataccc ctccagcacc agtaaccaaa atcactaccg ccgcagtggg
      721 aagggcagag cccataatgt cgctggtgtg ttgtaagctc cagccgcgac gtaaagccaa
      781 taaccagaat gccagcacca gcgcaatcat tagagctacc attggtgagc cgatcaactg
      841 tagcgtacca agcaggggat gcgaaggcgg catcagtgtt gcagaaaccg tacccgccat
      901 gataatcgcg ataggaataa caattagcga ggtgaccagc gcgacgcccg gtggatttat
      961 tttatcgctt aattttgtcg cgccttcctc actggccgga gccagttgca tctgttccag
     1021 tacctcgacc gacatcgcat attggcgctt attgattatt ttcgctgcaa aatagccaac
     1081 aatccctacg ggaatagaaa tcgcaatacc gatgatggtt agccagccga tgtctgcgtg
     1141 gagtaacccc gctgcggcga cagggcctgg atgcggcggt accgccacgt gaacagtgag
     1201 catgatccca gcgacaggca ggccaaattt gagtggtgat attttggcaa ccttggcaaa
     1261 accgtaaatg attggcgcaa gaataataaa gccgacatca aagaagacgg gaataccgag
     1321 gaagaacgct gccagagtca gcgcagcgat agttcgtttg tcacctaact tgcgactgaa
     1381 ataattagcc agtgactctg caccaccaga gtgttcgatc atacgcccca gcatagcgcc
     1441 cagaccaata ataatagtga cggaaccaag tacaccgccc atcccagcga tcatcacttt
     1501 acccacttcg cccgccggta tacccgccgc aagtgcgact aacaggctga cgaggagcag
     1561 agcaacgaat ggttgtacct ttgccttgat gaccagtagc aacagcatga ttacgccagc
     1621 taacgcaatg cataacaatg taattgtgga catgggaaac cctgtctgaa agttatagtt
     1681 aacctacccc atccgtagat ggggggatgt atgggtacgt tgtaattagg gattgagcga
     1741 attagcgcca ggcgtcaaac cagccaagcc cttcttcggt gaggccacga ggtttgtatt
     1801 cacaaccgat ccagccctga tatcccactt catcgaacag gcggaacagc cacggatagt
     1861 tgatttctcc atcgtccggt tcatgtcgat caggtagtcc ggcaatttgt gcgtgcgcat
     1921 atttcccggc gtagtcgcgg attaaatgtg tcaggttgcc atctactttt tgcgcatgga
     1981 aagtatccag ttgaataaac acgttatctc gcgcaacctc ttcaacaata gccagtgcct
     2041 gatactggct ggagaagaga taatgaggct taacgccggg gctgagtgct tcaaccaata
     2101 ttcgcttgcc gtgtggcgca aagcggtcgg cagcgtagcg gagattatca ataaatactg
     2161 cccggtaccg ttcagcatct tcgccagcgg gcacgacgcc tgccatcaca tggacttgtt
     2221 cacaattgag cgccaatgca tattccagtg ccaggtcgat gtctgcgcgt gcttcgtgct
     2281 cacgtccggg aagggcggat aatccccatt ctcccgcatt aatatctccg ggagcggtat
     2341 tgaacagcgc cagtgtcaga tggttttgca ccagttgctg ctggatttgc tgggtggagt
     2401 agtcataggg aaacagaaac tccacagcat cgaacccggc ttttcgcgct gcggcgaagc
     2461 gttcaataaa aggcacttcg atgaacatca tggataaatt agctgcaaaa cgaggcattg
     2521 cattaactcc ttaattccgc aatttcacct gcggtcagat aacggatcgg gcggtcaccg
     2581 agaataaaaa tcagctttgc cgtttcctcc agctcttcca tattgttggc ggcttcttgc
     2641 aggctttcac cgcaaaccac tgggccatga tttgccagta aaaaagcctg attgtctgct
     2701 gccagttccg ccagatcctg tgcgatgcgt ttatcgcccg gtcggtaata gggcaccagc
     2761 gggacattac ccatccgcat caccacgtat ggtgtgaacg gccgaataac gttattgctg
     2821 tccagccctt ccaggcagga aagcgccgtc gaccacgtac tgtgcaaatg cactaccgct
     2881 ttacagcgcg gattgttgcg atacagcgcc agatgaaaga gcacctcttt cgagggtttg
     2941 tcaccactta accattcgcc atccgcggtg actttggaaa gccgctgcgg atcgagattg
     3001 cccaggcatg aacctgtcgg tgtcgccagt aaattcccgt caggtaaaag cagcgacaga
     3061 ttgccagccg aaccggttgc atatccgcgc tgaaagaatg aactggcaat ccgcgtcatc
     3121 tcctctcgca aagactgctc tacttttgcg aaatcgctca tgataaaaac tctctttggg
     3181 ctcgtgaaaa aaaggcttca tcaccgaagt tgccagattt aagggcgagt gagacaggct
     3241 tatccagtgc gtttacccac ggcacgccgg gggaaatggt tgggccaata tgaaaacctt
     3301 ttatgcccag gctctgtgtg actacgccgg aggtctcacc gcctgcgaca ataaagcgtg
     3361 tcacgccttc cgctgctaac cgcgccgcta gttgagaaaa cagagtttct actgcctgac
     3421 tggctttttg tgcaccgtat tgctgttgaa ttgctgccaa tgcgtcagtg ctggcggtgg
     3481 caaaaaccag tggagcaagt acactttcct ggcccagaac ccactctgcc agctcgtgtg
     3541 cataagcggc cagaatttcg gttgagaggc agcgtgccac atcaacctca cgggctggtg
     3601 caatttgacg gtaatgtgcc acctggcggt tggtcatttg agagcatgaa ccggagagca
     3661 ctacgccgcg cccagcgagc ggacgccctg cttcgcgagc ctggttaccg ttttcttgcg
     3721 cccactgccg ggccaggcca atcgccagac cagaaccgcc cgttaccagt ggggcatcgc
     3781 gcaaggcttc cccctgaatt tccagatggt gttcggtcag cgcatcaagc accgcgtagc
     3841 gatagccctc ttgctgtaag cgagccagct cctgacgaac ggctttcaca ccttgttcga
     3901 aaacatgtgc tgaaacgacg ccgcagcgcc ctgtggattg cgattcaacc agacggggaa
     3961 gatagctgtc ggtcatggga tttaccgggt gatggcgcat cccggattcg gccagcagtt
     4021 gattcattac gaacaaatac ccctgataaa ccgtacgtcc gttgactggc agggccggag
     4081 agaagaccgt aaacggtgtg tcgagagcat ccattaatgc atcggtaact gggccgatat
     4141 tccctttcgc cgtactgtcg aaagtagagc agtatttgaa atagatctgt ttgcaacctt
     4201 gctgttgcaa ccagctcaga gccgccagcg attgctgtgt ggcttcaacc accggacagg
     4261 agcgcgtttt caggctgatc accagtgcgt cgattgcttc cggcatttta cctgttggaa
     4321 caccgttaat ttgtaccgtt ggtagaccgt tttccaccag aaaactggcg atatccgtcg
     4381 cgccggtaaa atcatcggcg ataacgccaa tcttgatcat gatttcgctc ccggtagagt
     4441 gatgccagag aaaatcttga taactgcgct atcgtcttct ttcccgtaac ccgcgttact
     4501 ggcgctggtg aacatattca atgctgttga ggccaatggc agcgggaagt gcagggcttt
     4561 ggcggtatcg gcaaccagac caagatcctt aacaaaaata tcgacggctg aatgcggggt
     4621 gtaatcgcca tccaccacat gacgcatccg gttttcgaac atccatgaat ttccggcggc
     4681 attggtcacg acgtcataca tcacatccag cgggatcccc gcacgggctg caagcgccat
     4741 cgcttcggct ccggcagcaa tatgtacgcc cgctaacaac tggtggataa tttttacggt
     4801 cgaacccagt cccggttctg ctccgatgcg ataaactttt ccggcaacgg cttccagcac
     4861 gggtgctagt cgttcaaagg caatatcgct accggaggcc atgacagtca tttcaccgtt
     4921 agcggctttt accgcaccac cagaaaccgg cgcatccagc atttccagac cgaatccagc
     4981 cagagcggta gcaatttctt gcgcatcagc actggcgata gtggaagaaa ccattactgc
     5041 cgtaccgggt ttcagatgtt gtgcaacgcc tttttcacca aacagcacct gtttaacctg
     5101 ggtcgcattg accaccagca ccagcagagc gtccagtttt tcggcaaacg tcgcggcgtt
     5161 atcagaaact ccgcaagcac cagcctcttt caacgtagcg caggcattgc tgttcaggtc
     5221 tgcgccccag gtagaaagac ccgcgcggac acatgacagt gctgctccca ttcccattga
     5281 ccctaagcca acgataccga catgaaactc cgatcccgtt ttcatctgct ctccttgtta
     5341 atttaagtga tattttgttt tgtgttgtga atataagcgc ggaaagataa agatatggtg
     5401 agctaattca caaaagttaa cattgcgtgt tattttatgt gaactaagcg ttagttgcgc
     5461 cgcgcacgtt tcgcaggcaa atagcgtaga atgtcagcag gacaaaggaa ggagcaaaag
     5521 ttgatacccg tagagcgtcg ccaaatcatc cttgagatgg tagctgaaaa aggcattgtt
     5581 agtattgctg aactaacgga cagaatgaat gtgtcacata tgaccattcg tcgggattta
     5641 caaaaactgg agcagcaggg agccgttgtg ctggtgtccg ggggcgtcca gtctccggga
     5701 cgcgtggcgc atgaaccttc tcatcaggta aaaactgcgc tggcaatgac gcaaaaagcg
     5761 gctattggca agctggcggc aagtcttgtt cagccgggac gttgtatcta tctggatgcg
     5821 ggaacgacca cgttagcgat tgcacagcat ctgactcaca tggagccact cacagtggtt
     5881 actaacgact tcgttattgc ggactacttg ctcgacaaca gtaattgcac aattattcac
     5941 actggcggcg cagtgtgtcg ggaaaaccgt tcctgtgttg gggaagccgc tgcgaccatg
     6001 ctgcgtagcc tgatgattga tcaggctttt atttctgcat cgtcatggag tgtgcggggg
     6061 atctctacgc cagcagaaga taaagtcacg gtgaaacggg cgattgccag tgccagccgc
     6121 cagcgagttt tggtctgtga tgcgacgaaa tatggacagg tggcgacatg gctggcgtta
     6181 ccattaagcg agtttgatca gatcattact gacgatggtc tgccggagag tgccagccgc
     6241 gcgctggcga agctggatct ctctttgctg gtagcgaaaa atgaataatg acctgcaata
     6301 acgccgggtt actcatgctt cacagaagaa gcatgagact actttatttt ataaaatgac
     6361 agccgcccgc ttttcggcga tccggtatct ata
//
LOCUS       DAZFRG010000253         6386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFRG010000253 DAZFRG010000000
VERSION     DAZFRG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.255"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..135)
                     /locus_tag="ACHKUC_004200"
                     /pseudo
     CDS             complement(37..135)
                     /locus_tag="ACHKUC_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(194..760)
                     /gene="satP"
                     /locus_tag="ACHKUC_004201"
     CDS             complement(194..760)
                     /gene="satP"
                     /locus_tag="ACHKUC_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO6620352.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(909..1622)
                     /gene="msyB"
                     /locus_tag="ACHKUC_004202"
     CDS             complement(909..1622)
                     /gene="msyB"
                     /locus_tag="ACHKUC_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6620353.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGEFQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1648..2052)
                     /gene="yaaI"
                     /locus_tag="ACHKUC_004203"
     CDS             complement(1648..2052)
                     /gene="yaaI"
                     /locus_tag="ACHKUC_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO6620354.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEEQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2423..4339
                     /gene="dnaK"
                     /locus_tag="ACHKUC_004204"
     CDS             2423..4339
                     /gene="dnaK"
                     /locus_tag="ACHKUC_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO6620355.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4428..5558
                     /gene="dnaJ"
                     /locus_tag="ACHKUC_004205"
     CDS             4428..5558
                     /gene="dnaJ"
                     /locus_tag="ACHKUC_004205"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6620356.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5663..5815)
                     /gene="mokC"
                     /locus_tag="ACHKUC_004206"
     CDS             complement(5663..5815)
                     /gene="mokC"
                     /locus_tag="ACHKUC_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO6620357.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1698 a         1546 c         1627 g         1515 t
ORIGIN      
        1 caagcggggg gatcagactg atgacaaacg caaaactgcc tgatgcgcta cgcttatcag
       61 gcctacgatc ttcttgcaat atattgaatt tgcacgattt tgtaggccgg ataaggcgtt
      121 cacgccgcat ccggcataaa caaagcgcac gttgttaaca atcttatccc ttcgagcggg
      181 gggagcttaa taattagtga gactcaccaa tcggcagaac ggtgcgacca aactgctcgt
      241 tcagcacttc acccatcgcc agatagattg cactggcacc gcagataaga ccaatccagc
      301 cggcaaagtg gatgatcgcg gcgttaccgg caatgttacc gatcgccagc agggcaaaca
      361 gcacggtcag gctaaagaaa acgaattgca gaacgcgtgc gcctttcagc gtgccgaaga
      421 acataaacag cgtaaatatg ccccacagac ccaggtagac accaaggaac tgtgcatttg
      481 gcgcatcggt cagccccagt ttcggcatca gcagaattgc aaccagcgtc agccagaaag
      541 aaccgtaaga ggtgaatgcg gttaaaccga aagtgttgcc ttttttgtac tccagcagac
      601 cggcaaaaat ttgcgcgatg ccgccgtaga aaatgcccat ggcaagaata ataccgtcca
      661 gagcaaaata acccacgttg tgcaggttaa gcagaatggt ggtcatgccg aagcccatca
      721 ggcccagcgg tgccggatta gccaacttag tgttgcccat aattcctcaa aaatcatcat
      781 cgaatgaatg gtgaaataat ttccctgaat aactgtagtg ttttcagggc gcggcataat
      841 aatcagccag tggggcagtg tctacgatct tttgagggga aaatgaaaat tttccccggt
      901 ttccggtatc agacctgagt ggcgctaacc atccggcgca ggcaggcgat ttgcagtacg
      961 gctggaattg tcacgcgata ggcactgccg ctgaccgctt taaccccatt taatgctgcg
     1021 cccacagggc ctcccagacc cgcgccgcgc agcaaaccat gcccaagcac gctcattgct
     1081 gcgtgggtgc gtaaaatgcg ggtcaattgg ctggaaagca aatgcgacac accttttgcc
     1141 aataatttgt ctttcatcag cagcggcagc agctcttcca gctcattcac cctggcatcg
     1201 accgcgtgca gaaactcctg cttatgttcc tcgtccattt tcttccaggt attacgcaga
     1261 aattgttcca gtaactgttg ctcaatttca aacgtagaca tctctttgtc ggctttcagc
     1321 ttcaatcgct ttgaaacatc gagcaaaatg gcgcgataca atttaccgtg tccgcgcagt
     1381 ttgttggcga tactatcgcc accaaaatgc tgaaattctc cggcaatcag ctgccagttg
     1441 cggcggtgtc gctcgggatg tccttccatc gatttaaaca gttcgttgcg catcagtacg
     1501 ctggagaggc gagttttgcc tttttcatta tgggtgagca gccgggcgaa atttgccaac
     1561 tgttcctcac tacaatgctg gagaaaatcc agatctgaat cattcaggta attaacattc
     1621 attttttgtg gtttctatat tctggcgtta gtcgtcgccg ataattttca gcgtggccat
     1681 atccgatgag ttcactgtat gaccggaaaa ggtgattttt gagacgcagc gtttgttgtc
     1741 gttatcgctg ttaatgttga tccagtcagt ggtttgctct tcttttattt ctgaaggaat
     1801 attcagggtc tgactggcgc ttcgggtggc tttgaaataa atcgatgcac cgcttaactg
     1861 caaatcgcca tggtcggctg agagttgtat gcgtttaaca atgcgacaaa caggaagttt
     1921 cagcgccaga tcgttggttt cgtttcgcgg cattgcaatg acgccgagga ttttatggtc
     1981 gtttgcctgc gccgtgcagc acagcatcag gctaatcgcc aggctggcgg aaatcgtaat
     2041 aacggatttc ataaggattc tcttggtggg gaggggtaag gggatgaaac tagtttactg
     2101 ctgataaaga gaagtttcag gtacgtaata ttttcttttt attacaattt tttgatgaat
     2161 gccttggctg cgattcattc tttatatgaa taaaattgtt gtcagtttca cgtcttgtcc
     2221 tgccatatcg cgaaatttct gcgcaaaagc acaaaaaatt tttgcatctc ccccttgatg
     2281 acgtgggtta cgaccccatt tagtagtcaa ccgcagtgag tgagtctgca aaaaatgaaa
     2341 ttgggcagtt gaaaccagac gtttcgcccc tattacagac tcacaaccac atgatgaccg
     2401 aatatatagt ggagacgttt agatgggtaa aataattggt atcgacctgg gtactaccaa
     2461 ctcttgtgta gcgattatgg atggcaccac tcctcgtgta ctggagaacg ccgaaggcga
     2521 tcgcaccacg ccttctatca ttgcctatac ccaggatggt gaaactctgg ttggtcagcc
     2581 ggctaaacgt caggcagtga cgaacccgca aaacaccctg tttgcgatta aacgcctgat
     2641 tggccgccgc ttccaggacg aagaagtaca gcgtgatgtt tccatcatgc cgttcaaaat
     2701 tattgctgct gataacggcg acgcatgggt cgaagttaaa ggccagaaaa tggcaccgcc
     2761 gcagatctct gctgaagtgc tgaaaaaaat gaagaaaacc gctgaagatt acctgggtga
     2821 accggtaact gaagctgtta ttaccgtacc ggcatacttt aacgatgctc agcgtcaggc
     2881 aaccaaagac gcaggccgta tcgctggtct ggaagtaaaa cgtatcatca acgaaccgac
     2941 cgcagctgcg ctggcttacg gtctggacaa aggtactggc aaccgtacta tcgcggttta
     3001 tgacctgggt ggtggtactt tcgatatttc cattatcgaa atcgacgaag ttgacggcga
     3061 aaaaaccttc gaagttctgg caaccaacgg tgatacccac ctgggtggtg aagacttcga
     3121 cagtcgtctg atcaactatc tggttgaaga attcaagaaa gatcagggca ttgacctgcg
     3181 caacgatccg ctggcaatgc agcgcctgaa agaagcggca gaaaaagcga aaatcgaact
     3241 gtcttccgct cagcagaccg acgttaacct gccgtacatc actgcagacg cgaccggtcc
     3301 gaaacacatg aacatcaaag tgactcgtgc gaaactggaa agcctggttg aagatctggt
     3361 aaaccgttcc attgagccgc tgaaagttgc actgcaggac gctggcctgt ccgtatctga
     3421 tatcgacgac gttatcctcg ttggtggtca gactcgtatg ccaatggttc agaagaaagt
     3481 tgctgaattc tttggtaaag agccgcgtaa agatgttaac ccggacgaag ctgtagccat
     3541 cggtgctgct gttcagggtg gtgttctgac tggtgacgta aaagacgtac tgctgctgga
     3601 cgttaccccg ctgtctctgg gtatcgaaac catgggcggt gtgatgacca cgctgatcgc
     3661 gaaaaacacc actatcccga ccaagcacag ccaggtgttc tctaccgctg aagacaacca
     3721 gtctgcggta accatccatg tgctgcaggg tgaacgtaaa cgtgcggctg ataacaaatc
     3781 tctgggtcag ttcaacctgg atggtatcaa cccggcaccg cgcggcatgc cgcagatcga
     3841 agttaccttc gatatcgatg ctgacggtat cctgcacgtt tccgcgaaag acaaaaacag
     3901 cggtaaagag cagaagatca ctatcaaggc ttcttctggt ctgaacgaag atgaaatcca
     3961 gaaaatggta cgcgacgcag aagctaacgc cgaagctgac cgtaagtttg aagagctggt
     4021 acagactcgc aaccagggcg accatctgct gcacagcacc cgtaagcagg ttgaagaagc
     4081 aggcgacaaa ctgccggctg acgacaaaac tgctatcgag tctgcgctga ctgcactgga
     4141 aactgctctg aaaggtgaag acaaagccgc tatcgaagcg aaaatgcagg aactggcaca
     4201 ggtttcccag aaactgatgg aaatcgccca gcagcaacat gcccagcagc agactgccgg
     4261 tgctgatgct tctgcaaaca acgcgaaaga tgacgatgtt gtcgacgctg aatttgaaga
     4321 agtcaaagac aaaaaataat cgccctataa acgggtaatt atactgacac gggcgaaggg
     4381 gaatttcctc tccgcccgtg cattcatcta ggggcaaatc aaaaaagatg gctaagcaag
     4441 attattacga gattttaggc gtttccaaaa cagcggaaga gcgtgaaatc aaaaaggcct
     4501 acaaacgcct ggccatgaaa taccacccgg accgtaacca gggtgacaaa gaggccgagg
     4561 cgaaatttaa agagatcaag gaagcttatg aagttctgac cgactcgcaa aaacgtgcgg
     4621 catacgatca gtatggtcat gctgcgtttg agcaaggcgg catgggcggc ggcggttttg
     4681 gcggcggcgc agacttcagc gatatttttg gtgacgtttt cggcgatatt tttggcggcg
     4741 gacgtggtcg tcaacgtgcg gcgcgcggtg ctgatttacg ctataacatg gagctcaccc
     4801 tcgaagaagc tgtacgtggc gtgaccaaag agatccgcat tccgactctg gaagagtgtg
     4861 acgtttgcca cggtagcggt gcaaaaccag gtacacagcc gcagacctgt ccgacctgtc
     4921 atggttctgg ccaggtgcag atgcgccagg gtttctttgc cgtgcagcag acctgtccac
     4981 actgtcaggg ccgcggtacg ctgatcaaag atccgtgcaa caaatgtcat ggtcatggtc
     5041 gtgttgagcg cagcaaaacg ctgtccgtta aaatcccggc aggggtggac actggagacc
     5101 gcatccgtct tgcgggcgaa ggtgaagcgg gtgaacacgg cgcaccggca ggcgatctgt
     5161 acgttcaggt tcaggttaaa cagcacccga ttttcgagcg tgaaggcaac aacctgtatt
     5221 gcgaagtccc gatcaacttc gctatggcgg cgctgggtgg tgaaatcgaa gtaccgaccc
     5281 ttgatggtcg cgtcaaactg aaagtgcctg gcgaaaccca gaccggtaag ctgttccgta
     5341 tgcgcggtaa aggcgtcaag tctgtccgcg gtggcgcaca gggtgatttg ctatgccgcg
     5401 ttgttgtcga aacaccggta ggtttgaacg agaagcagaa acagctgctg caagaactgc
     5461 aagaaagctt tggtggccca accggcgagc acaacagccc gcgttcaaag agcttctttg
     5521 atggcgtgaa gaagtttttt gacgacctga ctcgctaacc tccccaaaag cctgcccgtg
     5581 ggcaggccag ggtaaaaacc agggtgcgtt gaagatatgc gagcacctgt aaagtggcgg
     5641 ggatcactcc ccgccgttgc tcttactcgg attcgtaagc cgtgaaaaca gcaacctccg
     5701 tctggccagt tcggatgtga acctcacaga ggtcttttct cgttaccagc gccgccacta
     5761 cggcggtgat acagatgacg atcagggcga caatcatcgc cttatgctgc ttcattgctc
     5821 tcttctcctt gacctttcgg tcagtaagag gcactctaca tgtgttctgc atataggggg
     5881 cctcgggttg atggtaaaat atcactcggg gcttttctct atctgccgtt cagccaatgc
     5941 ctgagacaga cagcctcaag cacccgccgc tattatactg cgcttttaat tcacttctgt
     6001 tttatcaatt ctttttctga agacgcctcg catttttgcg gaataatatt tcaatacatc
     6061 aatttattta acttaaatat atccagggat tcacaaaaaa aggtgttttc ctctatcaaa
     6121 agtgaatggc taaaggaaat taattattaa aggttactgt aatactatag agagacaaag
     6181 aaatacgttc agcagctaat gctaagttca tcatgaaaaa tgcctataaa tcaaagggtg
     6241 atgtttaaaa cagacacatt ttctgaaaca cgatatccct ccgcccgttc gcaaaagtaa
     6301 tctgcgatct tcatcacgct tcgatagtca tctttaaata tactggcctg gtcattctta
     6361 ataaaaatga attgtctggt gtatta
//
LOCUS       DAZFRG010000254         6365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFRG010000254 DAZFRG010000000
VERSION     DAZFRG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.256"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1262)
                     /locus_tag="ACHKUC_004207"
     CDS             complement(<1..1262)
                     /locus_tag="ACHKUC_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620358.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLI"
     gene            1549..2301
                     /locus_tag="ACHKUC_004208"
     CDS             1549..2301
                     /locus_tag="ACHKUC_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6620359.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVCDVIRKNLDLDIHIQTVFCIKRVRYMGSIPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            2315..3391
                     /locus_tag="ACHKUC_004209"
     CDS             2315..3391
                     /locus_tag="ACHKUC_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6620360.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            3391..4389
                     /locus_tag="ACHKUC_004210"
     CDS             3391..4389
                     /locus_tag="ACHKUC_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO6620361.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAATWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            4394..5308
                     /locus_tag="ACHKUC_004211"
     CDS             4394..5308
                     /locus_tag="ACHKUC_004211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6620362.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGRDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            5336..5572
                     /locus_tag="ACHKUC_004212"
     CDS             5336..5572
                     /locus_tag="ACHKUC_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6620363.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKIFSMLLTIAETIIKKEMPVFIH"
     gene            5518..>6365
                     /locus_tag="ACHKUC_004213"
     CDS             5518..>6365
                     /locus_tag="ACHKUC_004213"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO6620364.1"
                     /translation="MNDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDAKQQ
                     PFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNISSDK
                     ESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSIDADG
                     RPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFNDWD
                     YSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGVTFG
                     PLIDGHT"
BASE COUNT         1930 a         1369 c         1381 g         1685 t
ORIGIN      
        1 atgatgagtc cggtaatcag gttaagtatc gcgggaatga tcgtcattaa caggcagatc
       61 ccccacaacg cctgtggtgt ttgttctacg ccaggttgat agtccataaa cgctaaagcg
      121 attccgataa accctcccgc cagtcccata ccaaactttt gaaaaaagag aatcccgcca
      181 aatgccagtc cagaaacacg ttgccccgtt ttcatttcac cataatccac tgactcagca
      241 atggatgccc aataaatggg aagctgaata tcagtcagaa aggtcaccac aatataaaat
      301 gtaaaagcta acactataga atttggttgc acgaaataca tcgcaccacc caccacgaac
      361 gccagtaatt gggtataacg aaatattttt attttgtcgt agagtcgagt aagccaggtg
      421 caagcgatca tagccagaac agaggcgatc acgcctgaag ttaaaaacgg tgaaattaaa
      481 gcatccccac cattcaggta atattttgca taatatgcag cgacagagtt acgaataatc
      541 ccgccaaaca taatgatcgc aattacagcg cccaaaatga gccattgatc atttttgatc
      601 agtaaccgaa actgcgcggc cataccgaga ctgggttttg gatggtagat gcgctctctc
      661 acgctaaaaa aacaatacag gcataaacac gttgccagaa ttcccattgc ccccattgct
      721 atttgataac ccagttgtac gttattcttc ccaagataca ttgcagataa aggaacaata
      781 attgttacgg cgaacatggc aatttttgtc ataacaaaac gataaccatt ggcgctcaat
      841 cgctcttgtg gatcatccgt gataacgctg attaatgaaa cataaggaat agcgattaac
      901 gtataggtca aggtaagtaa cacataggta gcccaggccc ataaaagctt aactgaataa
      961 tcggtattag gtgtggtgaa catcaaccaa atgcttatcc caaacggtat tgacataaaa
     1021 agcaaccacg gccgatattt tccccaacgt gtattcgttc tgtctgtcat tgttcccaca
     1081 actggatcaa tcacagcatc aaggatacgt acagaaaata acagaatacc taagtcgacg
     1141 ggtttcagac caaatacatc cgtgtaaaaa taagtgatga tcatggccaa agacatcatt
     1201 actatcgcga tagccatatc accagcacca aaacctatct tttcatttat tgtaagtttc
     1261 atacacagca ctcattgatt acgtgaactt atattaaaaa caaatatatc tgaactcatt
     1321 cagcacataa ctttacgagt gagcataaat gcattggttg ataccacaac aaaccattga
     1381 gtcaatattg agcaacatca cataaaaacc aatttcactt aatctcatga tttatatatt
     1441 taattgtggt tttttctgtt ttatgtcaca gatagtgaac aagcaccatg cgtaataaaa
     1501 gttaaagaat gtagagttgg tatcaaccac ttgaaatgag gctttgtcat gataaaaatt
     1561 tataaagcca gtaaaggtca gctaaagcat cgtgaaatct ttgatgaaat aaagaaaaaa
     1621 gtagataacg gtgaatggaa agaaggcaaa gccataccga cagaaaggga attgtcagaa
     1681 tattatttaa gtagcagaac aactattcga aaggcaatag aaagtcttaa acaacgcggc
     1741 tacctgcata gtgtccacgg gcaaggaaca tttgtattgc cttctcgtag tagagaaaat
     1801 catttattgc atagttttac cgacgatatt aaagctcgag gtggtatacc agcacagaat
     1861 attcttgaga taggctatgt tcctgtctgt gatgttatca gaaaaaatct cgacctggat
     1921 atccatatcc aaaccgtatt ctgtataaaa cgcgttcgct atatgggaag tatccccctg
     1981 ggtattcaga catcatggct tgcattaaat gacaggcaag aaataaccca agaagatctg
     2041 ctggcgacgg gttcgctcta tattttactg gaagaaaaac tcgctattaa acctttagaa
     2101 gcaacagaaa ttatctctgc ccggcttccc tcgccccttg agcgtcaatt actggaactg
     2161 actgccgatg atgtcgtatt aagttgtacc cgcgtcacgt tatccgtgga acgcaaacca
     2221 atggagtatg tagagatggt gtatcccgca agccggtact cctacaaagc acgaattaca
     2281 aaagacagtt ttagcgtgta acagagagaa tattgtgaac aaaatagaac aagctgtaat
     2341 ttataacaaa atattaggtt ctttagcctg cgcaggtatg ggcgatgcat taggcgcagc
     2401 gactgaactc tacagcattg atgaaataaa agctcaatgg ggaggttttt taaatgcctt
     2461 tgtttctcct cctgccgata cattcgctgg ctcattaaat ggtattgcgg gacttatcac
     2521 cgacgattcc agccaaatgt atgtgttttc agaagggtta attgaggctg gatttgataa
     2581 cttcaccaat aatgactggt tagcttgtct tctgcgttgg gccgatatgc agccctacgc
     2641 aaattataaa ggccccacaa cagaacaaat cgtcaaggca ctaaaagaag gtagaccaac
     2701 aaacaccatt gggcgtattg gaacatcttc tcgtcaggca cccaatgtcg gcaccacgaa
     2761 tggcgcagga atgcgtgtag cccctgcagg attgatctgg ccaggcaaga aagaaaaagc
     2821 gtgtcacctg gcgctaataa cttgcctacc ttcgcatgac accaatattg cgattgccag
     2881 cgcttgcgcc atcgctgcgg cgacatcaca agctatgtta ccagaagctt cgctcacatc
     2941 attactggac gccgcaatct ggggagcgaa ttacggcgaa cgcctggcca aacaatatgc
     3001 acgatgtgtc gcagggccat ctatcgccat gcgcatccaa cttgcagcgg acatcgccag
     3061 acgagccaat gacctggagt cttgtctgcg agaaatggaa ggtttggttg gtaactctgt
     3121 tgccgcccat gaatccattc ctgcggcaat cggtctattg ctgtactgca aaggagaacc
     3181 ctgggaaacc attcatgcct gcgccaatat tggcaatgac accgatagca tcgctacgat
     3241 ggcaggcgca attgcagggg catggcgcgg ttttgatgcg ctccctgaag ataaatatgc
     3301 ctttttccgc gccgtaaata ataaagattt cgatattgaa gcgatcgcat cgggtctgac
     3361 attgcttgca ttagaggcac aggagaaata atgaacatgc cagcccgtac tcctgttatg
     3421 ccattagcgc aaactcgtga ggaaataaat gctacggcgg catcgttacg ccatttggca
     3481 gctatctggg gtgaatgttt ttctactttt cagctcgata aacgtgctgt tgaacgcgtc
     3541 tgtatagtgg gatcgggcga ctcctggacg gtggcactat gtgccgccac ctggctgggg
     3601 aaatacacac atctgttttg ttacgcattg caaacgtggg attttcttca gactgattta
     3661 acccgttatc agaaagaaac gttaatcatt attctcagtg caagtggcag accatcaatg
     3721 accgttgacg ccctctgcca cgcagtatgc tcaaacgcgc aagtgctggg tgtgacgaat
     3781 tgccctggaa caccattttg cgcaataaca gaaaacatgc tctatacatg ggcgaataag
     3841 cagggtattc caacgcaaag tagtagcgtc acactttatg ccctcctgcg cttagcgcaa
     3901 aaattatgtc caggcttaac accactgcag attgaagatg atttggaagg gaaatttagc
     3961 cagattaatc agcactggca gcaaaagaag cgccattttt accaacaaaa agagataacg
     4021 tttcttggga gtggcttaag ttggggactg gcaatatcgg ggagcaattt actttcttgc
     4081 ggcccacaga ttcgtgcgac agcccttcca ctggaagaat tttatcattc attacgctta
     4141 catcaggctg gtgtcggtca gcattatatt ttactaccag ccacatcctg cgagtcccca
     4201 ttttaccttg caacccaaaa agcaattgtt gaacagggag cgacagcaga attaatcagt
     4261 tttatacctg acgccagtga gtccaacaat ttatttctgg tcatgcagtg gttatatgaa
     4321 atgtgctggc atttaagttg tgattatgtc gacgcaggcg ggcaacgcgt tagccatagg
     4381 gagaaataag actatgaaga aatttgatgt cgccgcttta ggcagtggaa atatagatat
     4441 ttttttatct atcccgtcac tccccactcg tggcggtaaa gttgtcggga cacgtctggg
     4501 tgaacaagct ggcggcacag tcgccaacag tgcctgtgcg atgggacaac tgggtctcaa
     4561 tgtggtatct gtttcatgtg tgggcaatga tcattcagcc tcgatcattc ttgatggatt
     4621 taaaaaatat catgtaaatt gtgatttcgt tcaggtaatt ccggaattga ttgccaatac
     4681 cgcgattatc tttattgatg aattaggtga aaaaacgttg gtttactcgc ctggtaggga
     4741 tcatgagtgg gataaagaaa aagccctgca ggcaattgcg caaagccgtt atttttatac
     4801 gatgccagca gatatcgaga aatttcgtat gctggccgaa tattcccgta gccagatgac
     4861 aaaagttgtc gttgatattg agcctcatat cattgcaaca cctgaacacc ttgccagaat
     4921 tttacacctt gctgatattg cgatatttaa ttatgacggt tttattcgtg gctatgcaac
     4981 agagccagat tttacacttc tgcatgatat acaagacgag tatcaacttg acgctgtcgt
     5041 ggttacgctg gatgttaggg gagtaattgc agtcaaaggc aatgagcaag taaaaatagg
     5101 tagttataca attcccgtta ttgataccac aggcgctgga gatactttta atggtgcatt
     5161 tgtttatagc ttaataaaaa atatgccctt aattgaagca ctaaaatttg cttctgccac
     5221 agcagcaata aatattactg cattaggtgc cagaggtcat ctgccgacac catcggaggt
     5281 taatcttttt ctttcacaac acgactaaaa tcatatatat acaaaggtaa atatcatgaa
     5341 aataagatat gccgctattt ttctggcatt accgctgtta tgcacaacag cgcaggcaaa
     5401 cagttttctt cattatgctg gggagtttaa cttccggacg ggagaaaagc agaacaatgt
     5461 cacaatcgcg gggctatcag ccatcacatt tgatgcaaaa aaaatatttt ctatgctatt
     5521 aacgatagca gaaacaataa taaagaagga gatgccagtc tttatacact aaaaatacag
     5581 gtatcatcca ggggcattga gcaagtgaat tttctcaacc agcgtccact gcttgatgca
     5641 aaacagcaac ctttcgtgac caatacggtg gatgccgaag gtctggccct gacacacaat
     5701 ggtaaatcat tactctggag ttcggagtta ggtgctccac tgcgtctgag cactctcgat
     5761 ggcgtaatgg agaaagattt tacgtcttta tttcctgctc gctttaatat tagttctgac
     5821 aaagagagca gcaatggtat tcgcagcggc aacgcctggg agggcctgac agttacacca
     5881 gatggaaaat cactttttat cgccgttgaa agcagcctta aacaggatgg gcctattgca
     5941 agtccaataa attcaggcac atcacgctta ctgcaatttt ctatagatgc agacggtcga
     6001 ccttcaaagc aattgcatga atatctctat attactgatc ctgttccgca agtgagtaag
     6061 ttcggtatta atgataatgg cgtatccgag gtcatggcgc tgaatgatca ccaattgttg
     6121 gtcatcgaac gttccggcag aaacgtcagt gctgggttca atgactggga ttactctgtc
     6181 agagtttata tggtcgattt aactgctgcc agcgatatca agaatattga cagtctgcaa
     6241 gattggtcca ataaatccac gttacaaccc gtcagtaaaa aattattaat tgattttgct
     6301 gattacactt cctctgcgga ttgtattgaa ggggtaacct tcggtcctct gattgatggg
     6361 catac
//
LOCUS       DAZFRG010000255         6359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFRG010000255 DAZFRG010000000
VERSION     DAZFRG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.257"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2306
                     /locus_tag="ACHKUC_004214"
     CDS             <1..2306
                     /locus_tag="ACHKUC_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6620365.1"
                     /translation="LDINGNDLTFHKLNAFDDGAIITSNGRLARLTLSLNEKTATIYH
                     GNFKNDLSVTNNTTNNSGDFIVDGGININDNFEQNGGKLFFQGHPVPHAVTSQSVANT
                     LKKLGDDSVLTQPVSFTQDDWETRQFSLKSLVLKDTAFYLGRNAQLTTDISADNSSVT
                     LGSEDVYIDLNDSNEKKVQVIPQQGQSKATKDSDKSTFYGNVSLTNNSTLNINEFFTG
                     GINSTDSTINISSPGATISEYSTLTRSALTVTDNAQLTSKAGLLSDDNVVLGSGATLS
                     LLNEQSTMPAFYSAAAWDLQGSGSTLNVGSGTMMSGDITAGSKATINIGVSTNQGSPL
                     GIYYSGDIKAPGANVSIDQTLWKLNSSSQMQNLALSNSQLVMTTDGKTSSNITVLDKL
                     IAENNILYVKPTRPLNEMSVGDIPLITAKNGTNNTQAFKTVPQQMGFHSMAPDVEVVN
                     TDGATQWRLKGFDVQRNNAALNESQRFMDIRYRNFLSEVNNLNRRMGDLRDTQGEAGA
                     WARFMTTSGSGEGGFTDRHMHLQIGADKKHHFDGGDLFTGIMMTVTDSKASGESYQGK
                     TRSVGGGLYASTLFNSGLYIDVVGKYVHHRDDYTMQTLGLGKADDTAHSWYLGAETGW
                     RYQWKPDVFIEPQAELVYGTLSGNTFNWQYNGMDVSMERKKAKPLIGRTGVEFGKTLD
                     GRDWQVTAKAGLSYQFDLRNTGATTYRDFAGESTVYNGKDGRMLANIGIDTRIKDNTR
                     IGLTIEKSAFGRYNVDNAINANIRYTF"
     gene            2463..3064
                     /locus_tag="ACHKUC_004215"
                     /pseudo
     CDS             2463..3064
                     /locus_tag="ACHKUC_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            3162..4553
                     /gene="selA"
                     /locus_tag="ACHKUC_004216"
     CDS             3162..4553
                     /gene="selA"
                     /locus_tag="ACHKUC_004216"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO6620366.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LENEQRFLEMLLK"
     gene            4550..>6359
                     /gene="selB"
                     /locus_tag="ACHKUC_004217"
     CDS             4550..>6359
                     /gene="selB"
                     /locus_tag="ACHKUC_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO6620367.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQIHRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT         1580 a         1476 c         1743 g         1560 t
ORIGIN      
        1 cgcttgatat taatgggaat gatctgactt tccacaaatt aaatgctttt gatgacggtg
       61 caattattac aagtaatggc agattggcca gactgacact gtccttaaat gaaaaaacag
      121 caactattta tcatgggaat tttaagaatg atttgtctgt gacaaataac accaccaata
      181 attcaggtga ttttattgtt gatggtggta taaatataaa tgataatttt gagcaaaatg
      241 gtgggaaatt gttttttcag gggcatcctg ttcctcatgc ggtgactagt cagtcagtag
      301 caaatactct caaaaaacta ggagatgact cagtattaac acaaccagtg tcatttacac
      361 aggatgattg ggagactcgc caattcagtt tgaagtctct tgttctgaaa gacaccgcgt
      421 tttatcttgg acgaaatgcg cagctcacta ctgatattag tgcagataac tcatctgtca
      481 cccttggcag tgaagatgtc tatattgatc tgaatgacag taatgaaaag aaagtacagg
      541 ttattccgca acaaggtcaa tcaaaagcga caaaggactc agataaaagt acattttatg
      601 gaaatgtatc tctgaccaat aactctactt taaatattaa tgaatttttt acgggtggaa
      661 taaatagtac agatagtact ataaatatat cttcccctgg tgcaactatc agtgagtaca
      721 gtacgttgac ccgttcggca ctgacagtca ccgataatgc acagcttaca agcaaggctg
      781 gtttactatc ggatgacaat gttgtgctcg gttcaggggc taccctctct ttgttgaatg
      841 agcaatcgac gatgccagcg ttttattcag cggcagcctg ggatttgcaa ggttctggat
      901 caacgttgaa tgttggttcc ggtacgatga tgagtggtga tattactgcc ggctcaaaag
      961 cgacaattaa tattggtgtt agtacgaacc aaggttctcc attaggcatc tactattccg
     1021 gagatatcaa agcacctggg gccaatgtta gtattgacca gacattgtgg aagcttaact
     1081 cgtcttctca aatgcaaaat ctggcactat ctaacagcca gcttgtgatg accaccgatg
     1141 gtaaaaccag cagcaatatt acggttctcg ataagttaat cgcagagaac aatattctgt
     1201 atgtaaaacc gaccagacca cttaacgaaa tgtctgtcgg tgatattccg ttgatcacgg
     1261 caaaaaatgg aaccaacaat actcaggcgt ttaaaactgt gcctcaacag atgggttttc
     1321 actcaatggc tcctgatgtt gaggtggtaa atactgacgg tgccacacag tggcgtctga
     1381 aaggattcga tgttcagcgt aataatgctg cactcaatga aagtcaacgt tttatggata
     1441 tccgttacag aaacttctta tctgaggtga ataaccttaa ccgacgtatg ggtgatctac
     1501 gcgatacaca aggagaagcc ggtgcctggg ctcggtttat gaccacttct ggttcgggtg
     1561 aggggggatt cactgatcgc catatgcatc tgcaaattgg tgccgacaaa aaacatcact
     1621 ttgatggagg cgatcttttt acaggtatca tgatgactgt tactgacagc aaagccagtg
     1681 gtgaaagtta ccaggggaaa acacgctctg taggcggcgg tttatatgca tccaccctgt
     1741 ttaactcagg attgtatatt gatgtggttg gaaaatatgt ccatcatcgt gacgattaca
     1801 caatgcaaac attgggactt ggtaaggcag acgatactgc tcactcatgg tatttaggcg
     1861 cagaaacggg ttggcgctat cagtggaagc ctgatgtctt tattgaaccg caggccgaac
     1921 tggtttacgg gacgttgtca ggcaatacct ttaactggca atacaacggt atggatgtca
     1981 gcatggagcg taaaaaggca aaacccttga ttggccgtac tggtgtggag tttggaaaaa
     2041 cactggatgg ccgcgactgg caggttacag cgaaggctgg tttaagttat caatttgacc
     2101 tgcgtaatac tggtgctacc acgtaccgtg attttgctgg tgagtccacc gtgtacaacg
     2161 gtaaagatgg tcgtatgtta gccaatattg ggattgatac acgcattaaa gacaataccc
     2221 gtatcggcct gaccattgag aaatcagcat ttggtaggta caacgtcgat aatgcgatca
     2281 atgctaatat ccgctataca ttctaatatt tagaatctca cacgaaaggt cgttttatct
     2341 acaaggcccg atatgcgtac gtatcgggcc atcttgcgtt tgtcatcaca ctcatatcaa
     2401 tatcacctgc ttttttgtaa ccttgggtca tactcatatt ttgttaacaa aaaggaaaca
     2461 taatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct attctgttgt
     2521 tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg gacaacggcg
     2581 tggcgcaatt taacccgtta gggaaagtgc cggtgctggt gaccgaagag ggcgaatgtt
     2641 ggtttgattc gccgatcatc gctgaatata ttgaattaat gaatgtcgct ccggcgatgt
     2701 tgccccgctg gagtcgttgc gagtgcgcaa aattgaggcg ctggcggatg gcattatgga
     2761 tgccgggctg gtatcggtgc gtgaacaggc gcgtccggcg gcgcagcagt ctgaagatga
     2821 attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag gatatctggt
     2881 cgatggcacg ctcaaaaccg atacggtcaa tctggcgact atcgccattg cctgtgccgt
     2941 cggatatctc aatttccgcc gcgttgcgcc aggctggtgt gtcgaccgcc cacacttagt
     3001 caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac cgccaaaggc
     3061 ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca atccgtggtt atgttaaacg
     3121 cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc gaaacgcgtt
     3181 ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc ttcctttctt
     3241 tgcgtgatac ttatggtcac acccgcgtgg tagaattgct gcgtcagatg cttgacgaag
     3301 cgcgagaagt gattcgtgat agccagacgc tgcccgcatg gtgtgaaaac tgggcgcaag
     3361 aagtcgatgc ccgtctgacg aaggaggcgc agagcgcgct gcgtccggtg atcaacctga
     3421 cgggaaccgt gctgcatacc aatcttgggc gagctttaca ggcggaagct gcggtggaag
     3481 ccgttgcgca ggctatgcgt tcgcctgtga ccctcgagta cgatctggac gacgccggac
     3541 gcggacatcg cgatcgggcg ctggcgcagc tgctgtgccg cattacggga gcggaagatg
     3601 cctgtatcgt caataacaat gcggcggcgg tgttattgat gttggcggcc actgccagcg
     3661 ggaaagaggt ggtggtatct cgcggcgaac tggtggagat tggcggcgcg tttcgtattc
     3721 ctgatgtcat gcgtcaggca ggctgcaccc tacacgaagt ggggaccacc aaccgcacgc
     3781 acgctaatga ttatcgtcag gcggtgaatg aaaataccgc gctgttgatg aaagtacata
     3841 ctagtaacta cagcattcag gggttcacca aagcgataga tgaagcggaa ctggtggcgc
     3901 tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg ctggttgatc
     3961 ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg gcgggcgtca
     4021 gtctggtgag cttctccggc gacaagttgt taggcgggcc gcaggctgga attattgttg
     4081 gtaaaaaaga gatgatcgcc cgcctgcaaa gccacccgct gaagcgtgca ttacgcgcgg
     4141 ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct ttatttacac cctgaagcac
     4201 tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc attcaaatcc
     4261 aggcacaacg tttacaggcc ccccttgccg cacattacgg cgcggagttt gcggtacagg
     4321 ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc ctgccgagcg
     4381 cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca ttagccgccc
     4441 gctggcgtga attgccagtg ccggtgattg gtcgtattta tgacggacga ttgtggctgg
     4501 atttacgctg ccttgaaaat gagcaacggt ttttggagat gttgttgaaa tgattattgc
     4561 gactgccgga cacgttgacc acggcaaaac aaccttattg caggcgatta ctggcgtaaa
     4621 tgccgaccgt ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg gctatgccta
     4681 ctggccgcag ccggatggcc gtgtgcctgg ttttatcgac gtacccggtc atgaaaagtt
     4741 tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg tggtggcgtg
     4801 cgatgacggc gtgatggcac agacccgtga gcatctggcg attttgcagc tgaccggtaa
     4861 cccgatgctg acagtggcgc tgaccaaagc cgatcgcgtg gacgaagcgc gtgttaatga
     4921 ggttgaacgc caggtaaagg aggtgctgcg ggaatacggt tttgctgagg caaaactgtt
     4981 tatcaccgca gcaaccgaag gtcggggaat tgatgccctg cgcgagcatt tgcttcagtt
     5041 accagaacgt gaacacgcca gccaacatag tttccgcctc gcgatcgacc gtgcatttac
     5101 cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta agcggggaag tgaaggtagg
     5161 cgattcactc tggctgactg gtgtaaataa accgatgcgc gtgcgtgcgc tacatgcgca
     5221 aaatcagcca acagaaactg cccatgccgg gcagcgtatc gcgcttaaca tcgcgggtga
     5281 tgcggaaaaa gagcagattc accgtggtga ctggctgctt gccgatgcgc cgccggagcc
     5341 gttcacacgg gtgattgtcg agcttcaaac ccatacaccg ctgacccagt ggcagccgct
     5401 gcatattcac cacgccgcca gccacgttac gggacgcgtt tcgctgctgg aagataacct
     5461 tgccgagctg gtgttcgaca cgccgctatg gctggcggat aacgatcgcc tggtgctgcg
     5521 cgatatctcg gcccgcaaca cgctggcggg ggcgcgtgtg gtgatgctta acccgccgcg
     5581 tcgcggtaag cgtaagccgg aatatctgca atggctggcg tctcttgcac gggcgcagag
     5641 cgatgccgat gcgttatctg ttcatctgga acgcggagcg gttaaccttg cagatttcgc
     5701 ctgggcacgg cagctcaacg gcgaagggat gcgcgaactg ctgcaacagc ctggctatat
     5761 tcaggctggt tatagcttgt tgaatgcgcc ggttgccgcc cgctggcagc ggaaaattct
     5821 cgacacatta gcgacttatc atgagcaaca tcgcgatgaa cctggtcctg ggcgagaacg
     5881 tctgcgacgt atggcgttgc caatggaaga tgaagcgctg gtactgttgc tgattgaaaa
     5941 gatgcgcgaa agcggcgaca tcctcagtca tcacggttgg ctgcatctgc cggatcacaa
     6001 agcgggcttc agcgaagagc agcaggccat ctggcaaaaa gcagagccgc tgtttggtga
     6061 cgaaccgtgg tgggtgcgtg atctggcaaa agagacggga accgacgagc aggcaatgcg
     6121 cctgacgcta cgccaggcgg cgcagcaagg aattattacc gcgatcgtta aagatcgtta
     6181 ttaccgtaac gatcggattg tcgagtttgc caatatgatc cgcgatctcg atcaggagtg
     6241 tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgca agctggcaat
     6301 tcagattctg gaatattttg accgtattgg ctttacgcgt cgtcgtggaa atgatcatt
//
LOCUS       DAZFRG010000256         6319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFRG010000256 DAZFRG010000000
VERSION     DAZFRG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.258"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            992..1591
                     /locus_tag="ACHKUC_004218"
     CDS             992..1591
                     /locus_tag="ACHKUC_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6620368.1"
                     /translation="MNTYNANSGYEMDPQLLTHFNHHLDSLFGVYSKLLPFRMDFAYR
                     KNTFSYGCVCRYAMCAEMLQLINEVGDKLVGYAWVMEYTERKGLHIHFVGYLNGQIHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1962..2354
                     /locus_tag="ACHKUC_004219"
     CDS             1962..2354
                     /locus_tag="ACHKUC_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6620369.1"
                     /translation="MYAKSFLALDGNGRLTGARAAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAEWTV"
     gene            2342..2767
                     /locus_tag="ACHKUC_004220"
     CDS             2342..2767
                     /locus_tag="ACHKUC_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6620370.1"
                     /translation="MDGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPKKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            3089..3412
                     /locus_tag="ACHKUC_004221"
                     /pseudo
     CDS             3089..3412
                     /locus_tag="ACHKUC_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3608..3908)
                     /locus_tag="ACHKUC_004222"
                     /pseudo
     CDS             complement(3608..3908)
                     /locus_tag="ACHKUC_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4086..4775)
                     /locus_tag="ACHKUC_004223"
     CDS             complement(4086..4775)
                     /locus_tag="ACHKUC_004223"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003825705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6620371.1"
                     /translation="MKTVLDSLKGKLIVSCQALENEPLHSPFIMSRMALAAAQGGAAG
                     IRANSVADISSIKEIVSLPMIGIIKRDYPGSEVFITATLREVDELMTVEPEIIALDAT
                     ARPRPGGQTLEELVTQIRARYPSVLLMADIATVEEAVTAERLGFDCVGTTLYGYTAET
                     ARHVLAENDCGFLREVLAAVSVPVVAEGNVDTPERAARCLTLGAHTVVVGGAITRPQQ
                     ITTRFIAAIAS"
     gene            complement(4775..>6319)
                     /locus_tag="ACHKUC_004224"
     CDS             complement(4775..>6319)
                     /locus_tag="ACHKUC_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/glucose-specific PTS transporter subunit
                     IIC"
                     /protein_id="HFO6620372.1"
                     /translation="FSCASSGGWFEKAQRFGKSFMLPIAVLPAAGLLLGIGGALSNPN
                     TLTAYPFLNVDWLQAIFTIMTSAGSIVFANLSVLFAVGVAVGLAKSDKGTAGLAALLA
                     FLVMNATINALLTLNGTLAHKNPGSVGQGMTLGIQTLETGVFGGVVIGLVTCTLHSRF
                     NKVSLPQFLGFFSGSRFVPIISSLAAIVVGAVMTVVWPHFQKLIFGLGGLVDATGYLG
                     TLLYGFILRMLGPLGLHHIFYLPFWTTALGGSEIVNGQLVEGTQRIFFAQLADPNTQQ
                     FYAGTARFMSGRFITMMFGLLGACLAMYHTAKPENRKVVAGLLLSAALTSFLTGITEP
                     VEFSFLFVAPVLYVIHAFFDGLAFMVAHILHITIGQTFSGGLIDFLLFGVLQGESKTN
                     WMYVPIVGIPWFFLYYFTFRYLINRFGWLTPGRENVTLVESGQPQSERAAAVIAGLGG
                     KENLEEVDCCATRLRVTVKESSKVDEAALKVTGARGVIIRGNGVQVIYGPHVTIIKNE
                     VEEILS"
BASE COUNT         1663 a         1610 c         1494 g         1552 t
ORIGIN      
        1 gttaattagt attatttata gaagtatgtt tatgtaactc taccgtgtaa atgaaccctg
       61 taattctagc gtgtaaatga actctgtcat tgtatcttgt aattctacct cgtaaataca
      121 ccttgtaacc aagtcctgca gaattctgtc tcatagaaat atgttcctgt gcagtgatat
      181 tctctgtagt agtacttgta actctgtcct taactctctc ctcagtaatg tagtcccgca
      241 acacctgaat ataaatctgt aatgttcaca cactgactgt aacgtgcttt gttaagtaat
      301 gtctgctatt tttttctgtt atttttcctg ttgttttctg tattttgttg tatggtgtaa
      361 taactattct gaacccgtca tcacctttat agctaagagt ataacttcgc cagtgttgat
      421 actgtggata acggtgatta aggaattact gttaatgatc tgttcctgca gctcacctga
      481 tgagttttgg tagtaatacg taaaagagta tgcctcatgt cattaaaaag tcttccagag
      541 tgttgtttaa ccctgtacct ctgttactca tgtagtacgt tgatatgtgt aatgctacgt
      601 taattgcaat atctggttat ggcgttatgc cttcagtatt aaaaacattt tttaccggag
      661 aaacagggaa gtggaaaggc tttcgggata atgtcaggcc attatgacgt taatcacagt
      721 acaagataac gagagggtaa tggaatttta aaccagagta gttccgctgt tgttcactgg
      781 aaaaagagat ttcccacacg cgttctgtcc gcttcagtct ggttaccaga tcttctggcg
      841 ttatatgcgc tgtatggttg ttacgtatgc tgttctctgc ctagttctga tacccgtgga
      901 agtcgttacc tgggatcacc ctacctgata acgtcttctt caaaaattac gccagcgctt
      961 cttcctgtgt tacagaacac gaggaagaaa catgaatact tacaatgcca attccggtta
     1021 cgaaatggat ccccagcttc tgacacactt taaccatcat ctggattcat tattcggggt
     1081 atattcgaaa ctgttaccgt tcagaatgga ttttgcttac cgaaaaaaca cattcagcta
     1141 cggatgcgtc tgcaggtatg ccatgtgcgc ggagatgctg caactgatta acgaggtagg
     1201 agataaactg gtcgggtatg cctgggtgat ggaatatacg gaacgtaaag ggcttcacat
     1261 tcattttgtg ggttatctga acggtcagat ccacaggagt tcgtatctgg tatccagatt
     1321 gatgggagat atctggaggc gggttaccga gggtaatggt tactaccact ggtgccggtt
     1381 taataaaaat tatccggtaa acattaacca cgtcattcac tattcagatc acaaagcggt
     1441 gaatgcctta cgctatgcca tcagctacct ggccaaacgg gaacagaaag aatgtggcat
     1501 catcctgaaa tgcagcgggc ttcctgaaaa aagtaaccgg ggtcgaccac gactggacag
     1561 ccccctgccg gggatatgtg ctctggtgta agcccctgaa cgacaagtac gggctggtgc
     1621 taatctttgc tgtcggttcc ggcggccact tcagcggcac tgcgcatttt ttacggcgtg
     1681 gccattccgt atgaaccata cggagattta accatgacac attctttacc attccttccg
     1741 gcaaccgata cagcacatcc cccaatctcc atctctgctt actgactgtt ttttacgtca
     1801 gtaggccatt gtgtgacaac cacagtgctg taacaacaac cagcctgttt tacccaaact
     1861 ccagtccgga ttttaactga gccgccattc acaccggatc acgctgcccg taccgggacg
     1921 gctgttgcct gcgtgtcgtc tggcgctgag gaacatatac tatgtacgca aaatcttttc
     1981 tcgcacttga tggcaacggg cgtctgaccg gtgcccgtgc cgcacagact gcaccttacg
     2041 atagctacac ctgccacctt tgcggcagtg cactcagata ccacccgcaa tacgacactg
     2101 aacgtccctg gtttgaacac actgacgacg ggctgacgga gcacggtcat gagtgtcctt
     2161 atgtcaggcc ggaacgcaga gaaatacggt tgattaaacg tctgcagcaa ttcgtcccgg
     2221 atgccttacc cgtagtacgt aaagccagct ggtactgcag acaatgtcac cacgattatt
     2281 atggggagcg gtactgcaca cactgccaga ccgggcgctt cagtgaggag ggggtagcag
     2341 aatggacggt ataaaaacgg ggctggggat cacgccgggt gagcacatta tcagtgccga
     2401 ctctgcgctc agcagaaata tccggcattg tttctgcctt tcctgtcatg gccggttgat
     2461 tctgcagaca gatgctcagg gggcgtggtt tgagcatgat ttacatgccc tgagtgagca
     2521 acagaaggcg gactgtgtgg tgctgaatcc gaaaaagtcc cacccttaca ttgaggatat
     2581 ggcgatgttt ttatcgccat taccggtcgt gctggagtgg cattgtgtga tgtgtgagca
     2641 gttttttcat gggaagaagt tctgtgaggc ctgtggtacg gggatttatt gcagagcggt
     2701 atgtacgaag tctgtttaca gctaccagcc agacttgttt gaggactgcg gaggtgcgag
     2761 tacttaatcc gaaatctccg ctctgcgcag ggcgttcaga ggtatcgcaa ctgaaccgcc
     2821 ccgggtttcc tggagattgt tttatctgtg aactcaggct gccagatcgt aagcgtaaac
     2881 cgaccgccgt atgtagccat tagacaagaa tgggtaactt agacgcccat ctgacacaga
     2941 cggacatcta agtatggaat tacaggactg gcaaaaagaa cctcgtaaaa agtattcgaa
     3001 tgaattgtaa gcgccccatc agcgacgtct tgtgaaaatt gtcctgtctg gcaacaatcg
     3061 cgcccatcta tattgatgga cacgaacgat gaattcccag acaacaaaag atattccctg
     3121 cttccgttct tatttgcctg atgcactgcg tttaagattt gaagataaac tgaccatccg
     3181 ggccatcgct cagcgtctgg gtctcagtca ttccacaata catacgcttt tttagcgatt
     3241 tcttgcatcc ggtatcgcat ggccattgcc cgattcagtt tcattcgctc aacttgacgc
     3301 catcctttat gccaacagaa agaaggaatt aacagagcct caaatcagag aaggctcatg
     3361 gcgaaaagaa cggcgaacca gctatagccg tgaatttaag gtccgtctgg cggcaagaaa
     3421 aaccagagcc gcgccactga tgcagtcact gtatgactgg atacagactc agatgaaaac
     3481 actgtcgcgt cactcagata cggcaaaagc gttctcctat gatgaactct ctcattaatg
     3541 cggcatatcg gatcggcaat aatctcccag ccactgtacc gccgcttgta aagtgctgct
     3601 gagccaatat cactttctga taggcttctg aagcgtcatg gacgttctta cgcaataacc
     3661 cgttatacca ctgtgaccac aaccagacgg taccgccgaa gcaattaaac aaccaataat
     3721 ggtgttgata agacacggaa ggatgtactg ctcgccattc agccataaca gttgcagggt
     3781 atatacagca gtaatggtaa aaccaacctt caccagccgt agtttttgca caatatcagg
     3841 caactggcga aggtggtaat cagcatcaac agggtataac cttcggaaat tttaaagtgc
     3901 agcgcaatca cccgcggttt gtcgtagctc agcgtggcga ataattcgtc cagcgcctca
     3961 atagtccccc tcaccttgca gggtctggaa aatctagtta atcgggtact gcataacact
     4021 ctcaatatgg ggataagcga taattatcgc ttatccccac caaagtatca taccccatcg
     4081 gtgctttacg aggcgatcgc cgcgataaag cgtgtcgtta tctgctgcgg acgagtgatt
     4141 gcccccccaa ccaccacggt atgagcgcct aatgtcaggc aacgtgcggc acgttccggg
     4201 gtatcgacgt tcccttccgc cacaaccgga acgctgaccg cggccaggac ctcacgtaag
     4261 aatccacagt cattttccgc cagcacatgc ctggcagttt ctgcggtata gccgtagagc
     4321 gtagtgccca cacagtcaaa acccagacgt tcagctgtca ccgcctcttc tactgttgct
     4381 atatctgcca tcagaagtac ggacgggtag cgagcgcgga tctgcgtgac cagttcttcc
     4441 agcgtctgac caccagggcg cgggcgcgca gtagcatcca gggcaataat ttccggctca
     4501 acggtcatca actcatccac ttcccggaga gtggccgtga taaacacctc actgccaggg
     4561 taatcgcgtt tgataatccc tatcatcgga agggagacaa tctctttgat agaactaata
     4621 tccgccacgc tgttggcacg gatacctgca gcaccacctt gtgctgccgc cagcgccata
     4681 cgtgacataa taaacggact atgtagaggc tcattttcca gagcctgaca ggagacgatt
     4741 aatttacctt tcagggaatc cagtacagtt ttcattacga taaaatctct tcgacttcgt
     4801 ttttaatgat ggtgacatga gggccataaa tcacctgaac gccgttgccg cgaataatca
     4861 cgccacgcgc tccggtcact ttcagcgcag cttcatctac cttactgctc tctttaaccg
     4921 tcacgcgcag gcgcgtggcg caacagtcca cctcttccag attctctttg cctcccagcc
     4981 cggcaatgac tgccgctgcg cgttcactct gaggctggcc ggattcaacc agggttacgt
     5041 tctcacgacc cggcgtcagc cagccaaagc ggttaatcaa atagcggaaa gtgaagtagt
     5101 aaaggaagaa ccacggtatt cccactatgg ggacatacat ccagtttgtt ttagattccc
     5161 cctgcaacac gccgaagaga aggaaatcga taagcccgcc cgaaaaggtt tgcccaatag
     5221 tgatatgtaa aatatgggcg accataaacg ccagaccatc gaagaaggcg tgaattacgt
     5281 ataatacggg cgcgacaaac aggaaggaga actcaacagg ctcagtgatt cccgtcagga
     5341 aagaggtcaa ggctgccgac aaaagtagtc cggcgacaac ctttctattc tccggctttg
     5401 cggtatggta cattgccaga catgcaccaa gcaggccaaa catcatggtg ataaagcgcc
     5461 cggacataaa gcgagcggta cccgcgtaga actgctgcgt attcggatcg gcaagttggg
     5521 caaagaaaat ccgctgcgta ccttcaacca actggccgtt gacgatttca ctgccgccca
     5581 gcgccgtcgt ccagaacggc aaataaaaga tatggtgtaa acccagtgga ccaagcatac
     5641 gcaggatgaa gccgtacaac agcgtcccca gatagcccgt tgcatccacc aggccgccca
     5701 gtccaaaaat gagtttctgg aaatgcggcc acaccaccgt cataaccgca cccactacta
     5761 tcgcggcaag ggagctgata atgggtacaa aacgagaacc actaaaaaaa ccgagaaact
     5821 gaggtagtga gactttattg aaacggctat gtaacgtgca ggtgacgaga ccaatcacca
     5881 ccccaccaaa cacaccagtt tccagtgtct gaataccaag tgtcatcccc tggccaactg
     5941 aaccggggtt cttgtgcgcc aacgttccat ttagcgttaa taaggcgttg atggtagcat
     6001 tcatcaccag aaacgctagc aatgccgcaa gcccggcagt gcctttatcc gatttcgcca
     6061 gccctacggc gacgccgacc gcaaagagta ccgacaggtt ggcaaacaca atagaacctg
     6121 cgctggtcat gattgtgaag attgcctgca gccagtcgac attcaagaat gggtaggccg
     6181 tcagcgtatt gggattcgac agagccccac caatccccaa caacaacccc gctgcgggca
     6241 ataccgcaat cggcagcatg aacgatttgc cgaaccgctg cgctttttca aaccatcccc
     6301 ccgatgaagc gcagctaaa
//
LOCUS       DAZFRG010000257         6261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFRG010000257 DAZFRG010000000
VERSION     DAZFRG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.259"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            236..517
                     /locus_tag="ACHKUC_004225"
     CDS             236..517
                     /locus_tag="ACHKUC_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO6620373.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(616..1152)
                     /gene="ssb1"
                     /locus_tag="ACHKUC_004226"
     CDS             complement(616..1152)
                     /gene="ssb1"
                     /locus_tag="ACHKUC_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO6620374.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1407..4229
                     /gene="uvrA"
                     /locus_tag="ACHKUC_004227"
     CDS             1407..4229
                     /gene="uvrA"
                     /locus_tag="ACHKUC_004227"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO6620375.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSTNVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4438..4794)
                     /locus_tag="ACHKUC_004228"
     CDS             complement(4438..4794)
                     /locus_tag="ACHKUC_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6620376.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4798..5214)
                     /locus_tag="ACHKUC_004229"
     CDS             complement(4798..5214)
                     /locus_tag="ACHKUC_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO6620377.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5325..6038)
                     /gene="aphA"
                     /locus_tag="ACHKUC_004230"
     CDS             complement(5325..6038)
                     /gene="aphA"
                     /locus_tag="ACHKUC_004230"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6620378.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1492 a         1640 c         1624 g         1505 t
ORIGIN      
        1 gggaagatat acccttaatg ccgctgaaaa aacacataat ctgtacggca ataaaagtca
       61 cggcctaatc tggctaacaa acaaccaatt gtgatatagt tcacaaaatt catgaaacaa
      121 acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt atcacgcctc
      181 ccgtgaacaa cgccgcgctg tccagggcat cagctctttt ccgaggaatt gtttgatggc
      241 taccctcacc actggcgtgg ttcttcttcg ctggcaactt cttagtgccg taatgatgtt
      301 tctggctagc acgctcaaca tccgttttcg tcgggctgat tatgtcgggc ttgcagtgat
      361 cagcagcggt ctgggcgtag tttccgcctg ctggttcgca atggggttgc ttggcatcac
      421 aatggcggat atcaccgcca tctggcacaa catcgagtcg gtgatgatag aagagatgaa
      481 tcagacaccg ccacaatggc caatgatttt gacttgatat gtagaagcct ccataacgga
      541 ggcttctttt ttatggctgg cagatgcttt aatcatccac cttaaaacaa tataacctat
      601 tgttttaatg acaaatcaga acggaatatc gtcatcaaag tccatcggcg gctcgttaga
      661 cggcgctgcc ggagcggact gctgcgggcg agactgcgcg ccgccgctga actgattgcc
      721 accctgcggc tgctgaggct gaccccaacc gccctgcggc tgaccaccac cgatattgcc
      781 acctgcagga gcgccaccac cctgacgacc acccaacatc tgcatggtgc cgccaacgtt
      841 caccacgact tctgtggtat agcgatcctg accggattga tcggtccatt tgcgggtacg
      901 cagctggcct tcgatataaa cctgagaacc tttacgcaga tattcgctgg ccacttctgc
      961 cagtttgccg aacagcacaa cgcggtgcca ttcagtctgc tctttcatct cgccggtcgc
     1021 tttatcacgc caggattcgg aagtagccag cgtaatgttg gcaactgcgc caccatttgg
     1081 catgtagcgt acttccgggt cctgacccag attaccaacg agaataacct tgtttacgcc
     1141 tctgctggcc atgttcgtgt ctcctgaaaa aaaatcgttc tgaataagtg taaacgcgcg
     1201 attgtaccat taccaatagc gcttttacta tgttgtgacc tcggttccgg gaaacaaacc
     1261 tggtcagaca ttgttacaca acactccggg taatgcattc caatactgta tattcattca
     1321 ggtcaatttg tgtcataatt aaccgtttgt gatcgccggt agcaccatgc caccgggcaa
     1381 aaaagcgttt aatccgggaa aggtgaatgg ataagatcga agttcggggc gcccgcaccc
     1441 ataatctcaa aaacatcaac ctcgttatcc cccgcgacaa gcttattgtc gtgaccgggc
     1501 tttcgggttc tggcaaatcc tcgctcgctt tcgacacctt atatgccgaa gggcagcgcc
     1561 gttacgttga atccctttcc gcctacgcgc ggcagtttct gtcactgatg gagaagccgg
     1621 acgtcgacca tattgaaggg ctttctcctg ccatctcaat tgagcagaaa tcgacgtctc
     1681 ataacccgcg ctctacggtg gggacaatca ccgaaatcca cgactatttg cgtctgctgt
     1741 acgctcgcgt tggtgagcca cgctgcccgg accacgatgt accgctggcg gcgcaaaccg
     1801 tcagtcagat ggtggataac gtgttgtccc agccggaagg caagcgtctg atgctgctcg
     1861 cgccaatcat taaagagcgc aaaggcgaac acaccaaaac gctggagaac ctggcaagcc
     1921 aaggttacat ccgtgctcgt attgatggcg aagtctgcga tctttccgat ccgccgaaac
     1981 tggaattgca aaagaaacat accattgaag tggtggttga tcgcttcaaa gtgcgtgacg
     2041 atcttaccca acgtcttgcc gagtcgtttg aaaccgcgct ggagctttcc ggtggtaccg
     2101 cggtagtggc ggatatggac gacccgaaag cggaagagct gctgttctcc gccaactttg
     2161 cctgcccgat ttgcggttac agtatgcgcg agctggaacc tcgcctgttt tcatttaaca
     2221 acccggcggg agcctgcccg acctgtgacg gtcttggcgt acagcaatat ttcgatcctg
     2281 accgcgtgat ccagaatccg gaattgtcgc tggctggcgg tgcgatccgt ggctgggatc
     2341 gccgcaactt ctattatttc cagatgctga aatcgctggc agatcactat aagttcgacg
     2401 tcgaagcgcc gtggggcagc ctgagcacga acgtgcataa agtggtgttg tacggttctg
     2461 gcaaagaaaa tattgaattc aaatacatga acgatcgtgg cgatacctcc attcgtcgtc
     2521 atccgttcga aggcgtgctg cacaatatgg agcgccgtta taaagagacg gaatccagtg
     2581 cggtacgtga agaattagcc aagtttatca gcaatcgtcc gtgcgccagc tgcgaaggaa
     2641 cccgtctgcg tcgggaagca cgccacgtgt atgtcgagaa tacgccgctg cccgccatct
     2701 ccgacatgag catcggtcat gcgatggaat tcttcaacaa tctcaaactc gcaggtcagc
     2761 gggcgaagat tgcagaaaaa atccttaaag agatcggcga tcgtctgaaa ttcctcgtta
     2821 acgtcggcct gaattacctg acactttccc gctcggcaga gacactttcc ggcggtgaag
     2881 cccagcgtat tcgtctggcg agccagattg gtgcaggcct ggttggcgtg atgtacgtgc
     2941 tggatgagcc gtctatcggc ctgcaccagc gcgataacga gcgcctgttg ggtacgctta
     3001 tccatctgcg cgatctcggt aataccgtga ttgtggtgga gcacgacgaa gacgcgattc
     3061 gcgccgctga tcatgtgatc gatatcggtc cgggtgcggg tgttcacggc ggtgaagtgg
     3121 tcgcggaagg tccactggaa gcgattatgg cggtgcctga gtcgttgacc gggcagtaca
     3181 tgagcggtaa acgcaagatt gaggtgccga agaagcgcgt tccggcaaat ccggaaaaag
     3241 tgctgaagct gacaggcgca cgcggcaaca acctgaaaga cgtgacgctg acgctgccgg
     3301 tgggtctgtt tacttgcatc acaggggttt caggttccgg taaatcgacg ctgattaacg
     3361 acacactgtt cccgattgcc caacgccagt tgaatggtgc gaccatcgcc gaaccggcac
     3421 cgtatcgcga tattcaggga ctggagcatt tcgataaagt gatcgatatc gaccaaagcc
     3481 caattggtcg tactccgcgt tctaacccgg cgacctatac cggcgtgttt acacctgtgc
     3541 gcgaactgtt tgcgggcgta ccggaatccc gtgcgcgcgg ctatacgcca ggacgtttca
     3601 gctttaacgt ccgtggcgga cgctgcgagg cctgtcaggg cgacggcgtg atcaaagtgg
     3661 agatgcactt cctgccggac atttacgtac cgtgcgacca gtgcaaaggc aaacgctata
     3721 accgtgaaac gctggagatt aagtacaaag gcaaaaccat ccacgaagtg ctggatatga
     3781 ccatcgaaga ggcgcgtgag ttctttgatg cggtgccagc tctggcgcgt aagctgcaaa
     3841 cgctgatgga tgttggcctg acgtacattc gcctcgggca gtccgcaacc acactttctg
     3901 gtggtgaagc ccagcgcgtg aagctggcgc gtgagctgtc aaaacgcggc accgggcaga
     3961 cgctgtatat tctcgacgag ccgaccaccg gtctgcactt cgccgatatt cagcaactgc
     4021 tcgacgtact gcataaactg cgcgatcagg gcaataccat tgtggtaatt gagcacaatc
     4081 tcgacgtgat caaaaccgct gactggattg tcgacctggg accggaaggc ggcagtgggg
     4141 gcggcgaaat cctcgtctcc ggtacgccag aaaccgtcgc agagtgcgaa gcttcgcata
     4201 cggcgcgctt cctcaagccg atgctgtaat cgttaaggcc gctttcagag cggcctcaga
     4261 ttattgacaa agtgcgcttt gttcttgccg gatgcggcat gaacgcctta tccggcctac
     4321 aaaaacatgc aatttcaata tattgcagag attacgtagg cctgataaag cgtagcgcat
     4381 caggcaattt tgcgtttgtc atcattctca ggccgctttc tgaacggtct tttcctttca
     4441 gagttgcacc agcaatttac gtttttcttc cggcagcaaa ttcaccgcct gctgataaga
     4501 cgcatccacc agataataga tttgcgaatc cggcagcgaa ccgtcgagat acacggtgct
     4561 ccagtgcgct ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag
     4621 ctccgccagc tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac
     4681 catcgcaaac agcacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct
     4741 ctgttctgcg cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttta
     4801 ctccccttga agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag
     4861 ccagatgcct tgccaggtac cggtctgaat acgcccttta tgcaccggca atacaagcga
     4921 tgttcccagc attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg
     4981 ctcataattt ccgttatcgg gaacggcgcg gaggaaaaaa cgctccatgt cgtggcgtac
     5041 ggtgggatcg cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg
     5101 cagtaagccg atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac
     5161 cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg
     5221 ctccttgata aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga
     5281 ttaaaaaaac agcattcggg agagtattac gctctcccgc tctgtcagta ttctgaattg
     5341 acgatcacct cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag
     5401 gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg cggcggtaat atcgttatcg
     5461 gaatcaccat aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttgc
     5521 cctggtttat cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta
     5581 tccgccagcg tttttgaaac cgtttctgtt ttggtcgggc tacgaccagt cacaaagaag
     5641 atcgcgtcac cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg
     5701 ctgaattcat cccagccatt gttcattttt tcccagaaca caggattttt cagataatct
     5761 tcgctttctg gcgagaaggt ttttttgccg cgccagaaac ccggactgga aaaaagtacc
     5821 gtatcatcga tatcaaaccc caccgccatt ggcggacgtc ctgcgaggct attttcaatt
     5881 tgtgcaaccg aaacccaatg aattggcgcc tgttcagcga gtttggctac gttagtaccc
     5941 ggattaagcg gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat
     6001 aagcaaacgg cactcaatgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta
     6061 agttgaaact tattattgtg cgtttgttga aaaaaccatc tgaccataac gccaaccgcg
     6121 ggggaaagga aggattatct gcggatttgt cacataaaaa gagcatgatc acatttggaa
     6181 tacctgtgcg ataaaccaca cattgccacg gttattctgc ttacacattc acacaatggc
     6241 actcttttgg ttctcgcaaa c
//
LOCUS       DAZFRG010000258         6252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFRG010000258 DAZFRG010000000
VERSION     DAZFRG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.260"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2778
                     /locus_tag="ACHKUC_004231"
     CDS             <1..2778
                     /locus_tag="ACHKUC_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6620379.1"
                     /translation="DGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTV
                     ILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAF
                     NRLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMD
                     GGADISGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWES
                     RTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKG
                     TSAVGGASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTS
                     SALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGS
                     GEYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEF
                     HTLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPL
                     VTAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQD
                     AETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTV
                     NSSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFS
                     DNYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDS
                     GAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAEL
                     VYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQF
                     DLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVD
                     NAVNASFRYSF"
     gene            complement(3346..6018)
                     /gene="rcsD"
                     /locus_tag="ACHKUC_004232"
     CDS             complement(3346..6018)
                     /gene="rcsD"
                     /locus_tag="ACHKUC_004232"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO6620380.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1491 a         1456 c         1744 g         1561 t
ORIGIN      
        1 gacgggcacc gtgttgactg gatggtgaac ggacttgcgg gagacgcact tcataaaatt
       61 ggtaagggaa cactggtggt ggccggcagt ggtgaaaatc ctggcaccct gaatacgggg
      121 gacggaacgg tcattctggc acagaaggct gatgctgcag gcagggtccg ggcattcagt
      181 gaagtcagta ttgtcagcgg acgaccagtt gtggtgctgc aggacagtca tcagattgaa
      241 ggggaccgca ttcgctgggg ataccgggga ggtacgcttg atatcaacgg aaatgacata
      301 gccttcaacc gcctggctgc ggctgatgag ggggcagtac tcacaagccg ggcgcgtcct
      361 gccacagtcc gtctggattt cagccagtca gggcagaagg ccgtgatgtg gcatggtcat
      421 tttaccggta acctcagcgt cctgaacaac acgtcatctg cggtggattt tatcatggat
      481 ggcggagcgg atatatccgg cagttttaca cagcagggcg ggggactgta tatccagggg
      541 catccggttg ttcatgctgt cagcagtgag gcggttgcga ctgcattgcg aaagcagggt
      601 gataattctg ttctgacgca gccggtatca tttgctcaga aggactggga gagcagaacg
      661 ttcagcatcg gtcaactgaa actgaagggg gcggcattca gcctttcccg caatgcgaca
      721 ctgacgggag atattgatgc tgataatgcc accatggtgc tgggaagtga ctcgctatat
      781 ctggatatga aggacggcac ggggagcagc agtgcgccgg ttaaggggac gtctgccgtg
      841 ggcggtgcat ccggtagcac tacatttcgc gggaatgtga atatgcgtca ttctgccctg
      901 acggtccgcg accattttac tggcagtata acggcatcgg acagccggat agctgtgagt
      961 tcagagaatg tgcgtctgga agggaattcc cgcctgacct cttctgcact tactgtcagt
     1021 gacggcgggc gtttacatgt gaagggggga ctggagacag atggtggtgt gacactggac
     1081 agaggaaccc ttttggtgga cgggggaagt gtcaggaatg atgtatatga acgtctgctg
     1141 gcctggtcag aggagcgtgg cgggctgaac ggaagcggtg agtatgattt tatgacgggg
     1201 gcagctggtc tcctgcggag ttatgtcaga gggagtgcag gtaatgtgaa tctgcagaat
     1261 gcggcctgga tgatgacggg aaactcatcc gtgaagcatc ttgagtcatc gggaagtgct
     1321 ctgtatttca gcaggccggg cggggagttc cataccctga cggcaggcag tatggatatc
     1381 agcgacagtg ttctggtgat gcggacggac cttaaccact cagaccagct tcgggtgaca
     1441 gaatcgctgc ggggtaagaa taacctgttg ttggttgatt ttactgagcg ttctgacggg
     1501 cagaaggcgc tgaatatccc tctggtgacg gcgccggccg gtaccggtgc ggatgtgttc
     1561 agtgtgaaaa cgcgtgatac cggcttcagt catatcacac ctgttgtcag ggctgaacag
     1621 gggacgggcg gcacggcctg gcagctgaat gttgtgcagc cggaaactgc cgcagagcct
     1681 gtggcgacgc ggcaggatgc agagacgccg aatcctgtgg aagctgtatc accgctccct
     1741 tcaccggttt ctgctccgtc atcggctccg gaggcgtcag tgacggatgt actgaccgga
     1801 gagaatgcgg gggaatcagg tgagtaccgt gatgagaccc ggtggctgct gacaggatac
     1861 cggagtacgg ttaatagctc cgcggtcagg gatgcgggga tgctgatgtc aatggggcac
     1921 cgtaacttca ttaatgaggt caacaacctg aacaaacgta tgggtgacct gcgggacatc
     1981 aacggcgagg ccggtgcatg ggcccgtatc atgagcggaa ccggctctgc cggcggtggc
     2041 ttcagtgaca actacacaca cgttcaggtg ggggcggata aaaaacacga actggacggt
     2101 ctggacctct tcaccggggt gaccatgaca tataccgaca gcaacgccgg cagcgacacc
     2161 ttcagtggta aaacgaagtc agtgggtgcg ggtctgtatg cttcagccct gtttgattcc
     2221 ggggcctata tcgacctgat tggtaagtat gttcaccatg ataatgagta tacggcgtcc
     2281 tttgccggac tcggcacaaa agactacagc tctcattcct ggtatgccgg tgctgaagtg
     2341 ggttaccgct atcatgtcac agaggatgcc tggattgagc cacaggcgga actggtctat
     2401 ggtgctgtct ccgggaaaca gttcagctgg aaggaccagg gaatgaacct caccatgaag
     2461 gataaggact ttaatccgct gattggccgt accggtgtgg atgtgggtaa atctttctcc
     2521 ggtaaggact ggaaagtgac ggcacgtgct ggtcttggct atcagtttga cctgctggcg
     2581 aacggagaaa cggtactgcg tgatgcatcc ggagagaagc gtattaaggg cgagaaagac
     2641 ggccgtatgc tgatgtctgt gggtctgaac gcagaggtcc gtgacaacat ccgttttggt
     2701 ctggagtttg agaaatcagc ctttggtaag tacaacgttg ataatgctgt caacgccagc
     2761 ttccgttatt cattctgacg gttgttactg aaggggatta tggcctgacg gctccctgta
     2821 aataaagaca ggcacagaag attgtgagtt actcagtgtt ccaccaaaaa gtaaggtcgc
     2881 aactctgtgc ctgcttcgta agtgtgcagt gagggccata tggttataac tgaagcatcc
     2941 gacgacgtag tgtctaccag agtgtcagat atgcaggaaa atatgagttc cgacagaaat
     3001 gcagtaaaaa aaggagtctc gcactcccct ccataaaact ccagggctgt aagttgtatt
     3061 aaaaagctga tttttcttta gcgatacaga cctcaataag tataacaccc ttcagttact
     3121 gtggttatgc acaggtgtta tttttaacag tgtggataaa cagtgtcgat tattcagatt
     3181 ggttatgggg catgtatggg acatttttaa tcggcgaaat acgtcgaagt tcgtcgatat
     3241 ggtaaacgaa tcatctatcc aacccttgaa aaacggcgct cctggacgat cttcgtcgat
     3301 ttttaaaatg caacaagtac gttgttcatg taataggcta ccttgctaca gcaagctctt
     3361 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     3421 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     3481 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     3541 atatagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     3601 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgcgccag
     3661 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     3721 gcacaattga ccaggcccaa tttcccgtac gccagactca tcatcgctta aaagcaagcc
     3781 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     3841 tctttcatcg ggtgtgatac aggttgcccc ccaattttct aactggcgag tgacaatatt
     3901 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta atcgctcttc
     3961 ttcctcttca acttccgggt cagctgcgag cattttgata tgcacagagt agcgtgtacc
     4021 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgcgcca gttgatcgct
     4081 cagccagaat gccagcgggt ccgccttgcc atagcgatcg ttttgggtct ggttgataaa
     4141 cgggaagtgc aaattatcca tttcatgaat gcttacgcct tctccagtgt ccagaatgcg
     4201 gaacgtcagg cggtcttcgg aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     4261 ttgcgttgag gtcacggcat attgcatcag tagtagcaaa atacgtcgta aggcatcgcg
     4321 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     4381 acgcttgatg gcaggcaaca ctgaaggcac tacttcatca attaaatcct gcacggagaa
     4441 cagctccgtc tcacttttcc agctatcgtc cgcaagcatg ttcgctaact gtatttcatc
     4501 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgccttccg gggcgttgag
     4561 tttagccgcg ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     4621 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
     4681 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
     4741 aacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
     4801 cccctgatgc tgctccgcca tggtagtgat gttttgcaga ttcaaatgcg gcagcaaatg
     4861 atcggcaatt ttgttactta tgactgtgcg gttcgattcc tgatcgtgaa ccagcaggcc
     4921 gagcggcagc agtgagacta tctcttcatt aattgcccgt aaaatgcgca attcgttatt
     4981 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
     5041 gccaaataat gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
     5101 caacgtatcc agcagtaagg tgccgtaagg aacctgccag accaggcgca tatcggtaga
     5161 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
     5221 cccttctttc tcattttcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
     5281 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
     5341 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
     5401 ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgcaa
     5461 catctctgca cgacgtgaat caacaatgtc gctgacggtc gattctttaa atccggaggt
     5521 gagatctttg aggggcaggg ttgagatcag caccagactg ttatcctgac cattcaggta
     5581 atacatcgac cacggtacat tttctgcgcc ccacaatgtg tccagatagg tggacatccg
     5641 ttgagtcatc tcaagcgttg agttgtcatg agagccaaag attaacgctt ccgttttgcg
     5701 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
     5761 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgccaggtca cgtaacgcca
     5821 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
     5881 cagccaggcg ttaacggcgc tttgtaccat tacacccatc gtcaccagta acacaatgat
     5941 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
     6001 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
     6061 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
     6121 agtgatattt atcatcctgc attgggggaa atagatgtac aagcgccatt ttgctgtaca
     6181 aataacctac aaaaagccta acgaaattaa ttgcaaataa acgaagattg ctggaaatta
     6241 tgctgatgtt aa
//
LOCUS       DAZFRG010000259         6233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFRG010000259 DAZFRG010000000
VERSION     DAZFRG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.261"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..3346)
                     /locus_tag="ACHKUC_004233"
                     /pseudo
     CDS             complement(65..3346)
                     /locus_tag="ACHKUC_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3464..4657
                     /locus_tag="ACHKUC_004234"
     CDS             3464..4657
                     /locus_tag="ACHKUC_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6620381.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4721..5974)
                     /gene="glyA"
                     /locus_tag="ACHKUC_004235"
     CDS             complement(4721..5974)
                     /gene="glyA"
                     /locus_tag="ACHKUC_004235"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO6620382.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
BASE COUNT         1468 a         1673 c         1608 g         1484 t
ORIGIN      
        1 cttattttaa ttatttgagc gtgagcggca gcgcgtgccg ctcaaatatt acagttaata
       61 gggtttagtg aacgtagcta aaaataaaag gcatcacggt ggtgaataat gccgctttcg
      121 gccaggctgg attaatcgcc atcaattcag cgcgagcaga ttcatagtgc gcaacctccc
      181 ctaatcccgc ggccgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta
      241 aatcgctata cagcgacaac aagtcaggct gcgagacggc aaagaaatcc gcttcgatac
      301 tggttttcgc catctctttc gcccactgtt tcatttcact aaacagttgc tgtgcggtgt
      361 gttgttcgcc cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat
      421 cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac
      481 acgtcgcttc agtttcatcg ccctgcgcgt tggcgcatac tgcctgccag aaccagatgt
      541 cgttgtcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg
      601 catgaagcag ctcgctggcc tgctgcggct ctctgtcatc aagatgctgc caggcgcgta
      661 ataactggtt gaggataaac tggctggtga ccttcccttc cccaccttcc cacgggtgga
      721 atttgcgcgt ggcgagaatg tccgccgctt tgtctgcctg accagtgaga tgccacaaat
      781 tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac
      841 gcgccagtcg tttctccggt gtgacaccgc ttaacttatc cagcaaatcc cgttcgaaaa
      901 gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct
      961 cataatcgtg ctgcttattc cacgcatgga tcgccaaccc gcgccagccg tcggcaaact
     1021 ccggcgacat ctctacgcaa cgctgccata aggcaatggc tttaccgtag ctacgcttgt
     1081 tgtagtagaa gcaggccagt aaatgccggg caaaccagca ctcttcgata ctctccagcg
     1141 ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaaggca tcaatagctt
     1201 ttacgaccag ttctccgcgt tcagcttttg gtagcaaact ggcttgcagg taaagcggta
     1261 gcgtgcgctg acagtccagt gcgttcagca tatctgccgc cagggcgggc atgccccagt
     1321 taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacaa agacctcgcc
     1381 actgtacgag ggcagattcg ctacgaccat cgaaccagtt cagccaccac agagtagcgt
     1441 tcagcggata atcacgcagc agtttctcgc gctgcaaacg cgcgttgtcc tgacgaccac
     1501 ttaacaccag tagcaggtta tgcaggcaaa gcacttcctg attggttggg caggcgcgaa
     1561 gactttgttg gcaaaaatcc agaccagcgt caaagttacc attacgcgcc gccagccgtg
     1621 ccagaccata atagccgcct gctttactgt tgccgctcca gaccgcacgc cagaaatcct
     1681 cttcggcttg ttgatattgc ccctgacgtt cgtaagcact ggcgcgaatc aaactcgcct
     1741 gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagcgcctga gtggcatacg
     1801 ccaccgcttg cgggaaatcg gcacggttat attccagcat cgccagcgcc aggttacagc
     1861 gataatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt gaacggctcg
     1921 cgtgatgata ttgctccaga tgttgaccga taaaccaggc ttcatctgta ctggtaatgt
     1981 cttgtgctgc cagtggcgct ttggcgacgt ccggcaacgg caacgcttgc gactgatgtt
     2041 catgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc agcctttcag
     2101 gattgatacc gtgcaacacg ccctagatgg cggtcgctgg tgtcagggcc acggcatcat
     2161 cgagcaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg ttcaacggag
     2221 agatggcata cagcccccac tcaatccccc gctcactacg ctggagtttt atcaccgcat
     2281 cgcgggaggc attttgcacc atgcccagag aatgataagg caggaaatac tgctcgaaac
     2341 gcttctcctc gtaagcatca agccaggtaa aatcaggctg gttatcggca aaaataccgg
     2401 tcatcagttc gatatacggg ccgttattgt cagtcaggct cttatcccac gcctggccaa
     2461 attcactgtg tccccagctc cactgttttt tacctggcgc aatatggtgg ttggcaacgt
     2521 gtagcaaacc accatcttca tcgtgacacc acgcgccaac aaaatcgtac tgtgattttt
     2581 cagccatata tgaggttgga acgggcacat ttttatagcg agaaatgtcc actccggcgg
     2641 agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg gcccgtttac
     2701 cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct tcaccccctt
     2761 tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc
     2821 gactggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta
     2881 aaccgtgcat cggctccgtt tcgccgaccc acaccgtctg tgcgccgtcg tcatgggctt
     2941 cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact
     3001 caatcccgcc ggagatccac ggccccagca gtcccaccag cgccggttta atgacttcat
     3061 tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc cgaccgccca
     3121 gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgccaggatt
     3181 tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg
     3241 atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat
     3301 aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat
     3361 taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt
     3421 tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa
     3481 caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa
     3541 gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cggtcagtaa
     3601 tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat atcgacgatg aaagccagac
     3661 gcgcgggcat agtagcggta catggctgat tgcgccggaa ggagactgga cgctgtgcct
     3721 gaacgtgacg cccaccagca ttgagtgtca ggtcgctaat gcctgtttaa gtccgaaagg
     3781 cgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt ccgaaatcga
     3841 aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc tggctctggc
     3901 aatccacggt caggttgatc cagtgaccgg cgtgtcgcaa accatgccgc aagcgccgtg
     3961 ggcaacaccg attgaggtga agtacctgct ggaagagaag ctgggcattc gggtaatggt
     4021 cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt cgcaggaacg
     4081 ggatttctgc gtaatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca
     4141 aatttatcgc ggcagtttgt atggtagcgg acagattggt cacaccatcg ttaatccgga
     4201 tggcgtcgtc tgcgactgcg ggcgttatgg ctgcctggaa accgtcgcct cgttaagcgc
     4261 attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttgatcc
     4321 tgaaaaattg actaccgcgc agttaatcgc tgcctggcag agtggagaac cgtggatcac
     4381 cagttgggtt gaccacagtg ccaacgccat tggtttgagt ctgtataact tcctcaacat
     4441 cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agaactggct
     4501 taataccatt attcgccaga cgggatttaa cccgttcgac cgcgacgaag gaccgagcgt
     4561 gaaagcgaca cagattggct ttgggcaatt aagccgtgca caacaggtac tgggaattgg
     4621 ctatttgtat gttgaggcgc agttacggca gatttgatgg cgcgataacg tagaaaggct
     4681 tcccgaagga agcctttttt caacagatta accgtttcgc ttacgcgtaa accgggtaac
     4741 gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca tcattgatgc
     4801 tgtccagcac gtcacacatc cagccagcca gttctttcgc ttcggcttct ttaaagccac
     4861 gacgggtaat cgccggagta cctacacgaa taccggaagt cacaaacggg ctcttcggat
     4921 cgttcggtac gctgtttttg ttcacggtga tgttagcacg gcccagagcg gcgtctgctt
     4981 ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta tcagtaccac
     5041 cagaaaccac tttgtagccg cgttcgagga acacttctac catcgctttg gcgtttttcg
     5101 cgacctgctg ctggtaagtt ttgaactcag gttccattgc ttctttcaga gcaaccgctt
     5161 taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg gcagagttca
     5221 gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg cgcggacccg
     5281 ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg ttagggtaga
     5341 caccagcggc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa gcaccgatgc
     5401 tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca gagaagccac
     5461 cgataatcat tttcggtttg tgttctttgg cttgtttttc cagatcggcg tagtcgatat
     5521 gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag aagttaaccg
     5581 gagaaccgtg agtcaggtga ccgccatgcg ccagattcat acccagaacg gtatcgcctg
     5641 gttccagcaa cgcggtgtag accgcaaagt tagcctggga gccggagtgc ggctggacgt
     5701 tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt tcaacgatat
     5761 caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca tatttgttgg
     5821 tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg gaggcgatca
     5881 gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac agttcggcat
     5941 cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc agctaacaat
     6001 aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat aaccgaatca
     6061 ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc aggctacgca
     6121 aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg tttgtgatgc
     6181 aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt aca
//
LOCUS       DAZFRG010000260         6204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFRG010000260 DAZFRG010000000
VERSION     DAZFRG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.262"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..459
                     /locus_tag="ACHKUC_004236"
                     /pseudo
     CDS             166..459
                     /locus_tag="ACHKUC_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(523..948)
                     /gene="tnpA"
                     /locus_tag="ACHKUC_004237"
                     /pseudo
     CDS             complement(523..948)
                     /gene="tnpA"
                     /locus_tag="ACHKUC_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            956..2153
                     /gene="insQ"
                     /locus_tag="ACHKUC_004238"
                     /pseudo
     CDS             956..2153
                     /gene="insQ"
                     /locus_tag="ACHKUC_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            2197..2304
                     /locus_tag="ACHKUC_004239"
                     /pseudo
     CDS             2197..2304
                     /locus_tag="ACHKUC_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2288..2926)
                     /gene="ytfB"
                     /locus_tag="ACHKUC_004240"
     CDS             complement(2288..2926)
                     /gene="ytfB"
                     /locus_tag="ACHKUC_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO6620383.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTSQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3145..3765
                     /gene="fklB"
                     /locus_tag="ACHKUC_004241"
     CDS             3145..3765
                     /gene="fklB"
                     /locus_tag="ACHKUC_004241"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6620384.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGTIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4074..5486
                     /gene="cycA"
                     /locus_tag="ACHKUC_004242"
                     /gene_synonym="dagA"
     CDS             4074..5486
                     /gene="cycA"
                     /locus_tag="ACHKUC_004242"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO6620385.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5531..6193)
                     /gene="ytfE"
                     /locus_tag="ACHKUC_004243"
     CDS             complement(5531..6193)
                     /gene="ytfE"
                     /locus_tag="ACHKUC_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO6620386.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
BASE COUNT         1450 a         1512 c         1626 g         1616 t
ORIGIN      
        1 aaatagcgga tggtgaaaga tattaatatc aaaaatcgct cattatggat ataacgatgc
       61 acccggggag aaaatgactt ccacgcctta ccattcttgt tgtgcggtat gtaattattc
      121 tacgctgtcg gtatcggtgt attcgttaat cagcaggtta gccagccaca aaaagccatt
      181 gagaaaatta ttgattttac atgggattat tatattgcta atccttggtt tttaaaaatt
      241 gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa aatcacagcg tctcttagaa
      301 attaaccatg cgcacttgca gttaatggaa tccttattgg atgaagggaa gaaacacaat
      361 atttttaagc cagatatcga cccattgcag gtgaatatta atattgctgc gcttggcgga
      421 tattatttga tcaaccagca tacgcttggc ctggtttatc cggtgcgccg taaaaccccg
      481 tccttcaggg cggggatata cggcgcggtt ttccacctga ccaggtgttt gctgttgttc
      541 aatgtattga cggatggcgg atattggcgc acctccgcag ctactggcaa aatagccagg
      601 actccacaaa acgcctttgt agtaatacct gactgcaata tctggtcgat ctcgtcgcag
      661 taacctactc gatacgcctt tgaggctgtt taccagactg gatatcgcca gtttgggagg
      721 atagttaatt aacaaatgga cgtgatctgg ttcgccatcc atttcaacca gttcagtttc
      781 aaaatcagca catacatttg aaaagtaagt gcgtagtttt tctgtcgcgt catagtcaaa
      841 aatctgacgt cggtatctgg tgacaaagac cagatgaaca tgcatcagga aaacacaatg
      901 ccttccacgc cggatatcgg tttctttttt cacagaccaa agtatcattt ggcctgtgaa
      961 acgactacag gcatttaaat tccagttaag accaggtggt caacaggagc gtgaaatgag
     1021 gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat catgaggccg
     1081 gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaacgcca
     1141 ctgaaacgca atggcttaaa gattctccct cacagccatt gcaacagtca ctgaaagacc
     1201 ttgagcgggc ttacaaaaac ttcttccaga atcgggctgc ttttccccga ttcaaaaagc
     1261 ggggacagaa tgatgcattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc
     1321 gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag gttacgggag
     1381 ttgtgaaaaa tgtcactgtc agccagtcct gcggtaagtg gtacatcagt attcagactg
     1441 aaaatgaagt atcaactccg gttcaccctt cagcattaat ggtcggactg gatgctggcg
     1501 tggctaaact cgccacgctg tcagatggca cagtctttgg gcctgtaaac agttttcaga
     1561 aaaaccagaa gacgctggcg agacttcagc gacagttaag ccgcaaggtc aaattcagca
     1621 acaactggca gaagcagaaa cgcaaaatac agcgactgca ttcccgtatc gcaaatatcc
     1681 gcagggacta ccttcacaaa gtcacaacga ccgtcagcaa aaaccacgca atgatagtca
     1741 ttgaggattt gaagatcagc aacatgtcaa agtcagcagc gggtacggtc agccagccgg
     1801 ggcgcaatgt ccgggcaaaa tcaggtttaa accgttcgat actggatcag ggctggtatg
     1861 aaatgcgccg ccagcttgag tacaagcagc tctggcgtgg cggtcaggtg cttgctgttc
     1921 cgccagcgta cacaagccag cgttgcgcgt gctgtggtca tacagcgaaa gaaaatcgcc
     1981 tgtcacaaag taaattcaga tgccaggcat gtggatatac agcgaacgct gatgtaaatg
     2041 gcgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggagag atggtgcagt
     2101 caggccgccc gttgaagcag gaacccaccg aaatgattca ggcgacagcc tgaacgtagc
     2161 agggatccac gtccttcagg gcgtggagga tgtcaacaca tcagtatggt ttcaccccag
     2221 gcacaggaag ccagacgtaa ggtcatcaaa gagacaatcc ttagctggct tttggttgac
     2281 ccgtcatcta ccgcgcacga ataaaactgc catccggctg gcgggtgaac aggacctgtt
     2341 gattattccc cgtatcaatg gttaagcccg tcaccacgcc gctggcgttc tggcggattt
     2401 tcaccatctg cccgttttgc aaattactca gcggcttacc cgcgccttct acctgcgcca
     2461 ttgcatagac atcggtagcg ggcagtccgt ggtcgcggaa cagctgcgcc atcgttttac
     2521 cgggttcgac acggtaagag cgccattgat tatcaattcc gctatctggc tggaatggtt
     2581 gcgtttggga agtttgcggc tgctcttctg gctggccttc ctgtataggt tctggagcga
     2641 ccggagccac ctgatcagga tcattttgtg gtgtgactaa ttgcgcccgc agctgttctt
     2701 ctgtcggcgg ttgggattgc gactgaatat ctaactgcgc ttcacgcgtc accaccggcg
     2761 cgttgggcgt atcatcagat ggaagcagaa atccgaccac caatacaatg gcggcaataa
     2821 tgatcccccg acgatgcatc ggcggcagcg ggtccatgaa gcgaaaattg tccggcgcgt
     2881 gccagacttt ctccagggtt ggttttagtt caaagcgccc gggcatggtt tcctcctgct
     2941 ccgcgtcttg ttcctcaatc atagcctatg aataagctaa cgctatgatg tccgtggtaa
     3001 acccgccttt attatattca tacgggatat tgctattgtt tctttttccc cgggatttgt
     3061 catcattcct gcgacaaagt tttacccaaa gaagtgtggc tgatatgctg cccgctactt
     3121 ttatacccta agaaaggaaa tacgatgacc accccaactt ttgacaccat cgaagcgcaa
     3181 gcaagctacg gcattggttt gcaggtaggg caacaactga gtgaatctgg cctggaaggg
     3241 ctgctgccag aagcactggt tgcaggtatt gccgatgcgc tggaaggcaa acatccggct
     3301 gttccggttg atgtggtgca tcgcgcactg cgtgaaatcc acgaacgcgc cgatgccgtt
     3361 cgtcgtcagc gtttccaggc gatggctgct gaaggtgtga aatatcttga agaaaatgcc
     3421 aaaaaagaag gtgtgaacag caccgaatct ggcctgcaat tccgcgtgat caaccagggt
     3481 gaaggcacaa ttccggcacg taccgaccgc gttcgtgttc attacaccgg taaactgatc
     3541 gacggcaccg tgtttgacag ctccgttgct cgtggtgaac ccgctgaatt cccggttaac
     3601 ggcgtgatcc ctggctggat tgaagcactg actctgatgc cggtaggttc taaatgggaa
     3661 ctgactatcc cgcaggaact ggcatatggc gagcgtggcg caggcgcgtc catccctccg
     3721 ttcagcaccc tggtgtttga agtcgaactg ctggaaatcc tctaagcagc gcattctgtt
     3781 cccctcgaac cagaggggag caggcatgca gcaataaacc cttcagtttg ccaaacggcg
     3841 ctattttgtg ttgcaaagac cccgtaagcg tgtatttttg tgagctgttt cgcgttatca
     3901 ccgtgatatg acactcactt taaacataaa attaacatta gatctaaatc ttagtattca
     3961 tcccgcgtat tgttacctaa tatcgatgag tcccgataca gattcgtcgt atcatagact
     4021 gactaaaggc cgtagagcct gaacaacaca gacaggtaca ggaagaaaaa aacatggtag
     4081 atcaggtaaa agtcgttgcc gatgatcagg ctccggctga acagtcgcta cggcgcaatc
     4141 tcacaaaccg acatattcag cttattgcca ttggcggtgc cattggtacg gggttgttta
     4201 tggggtccgg caaaacgatt agccttgccg ggccgtcgat cattttcgtt tatatgatca
     4261 ttggttttat gctctttttc gtgatgcggg caatggggga attgctgctt tcgaatctgg
     4321 aatacaaatc ttttagtgac ttcgcttccg atttactcgg gccgtgggca ggatatttca
     4381 ccggctggac ttactggttc tgctgggttg taaccggtat ggcagacgtg gtggcgatca
     4441 cggcttatgc tcagttctgg ttccccgatc tctccgactg ggtcgcctcg ctggcggtga
     4501 tagtgctgct gctgacgctc aaccttgcca ccgtgaaaat gttcggtgag atggagttct
     4561 ggtttgcgat gatcaaaatc gtcgccatcg tgtcgctgat tgtcgtcggc ctggtcatgg
     4621 tggcgatgca ctttcagtca ccgactggtg tggaagcgtc attcgcgcat ttgtggaacg
     4681 atggcggctg gttcccgaaa ggtttaagtg gcttctttgc cggattccag atagcggttt
     4741 tcgctttcgt ggggattgag ctggtaggta caacagctgc ggaaaccaaa gatccggaga
     4801 aatcactgcc acgcgcgatt aactccattc cgatccgtat cattatgttc tacgtcttcg
     4861 cgctgattgt gattatgtcc gtgacgccgt ggagttcggt agtcccggag aaaagcccgt
     4921 ttgttgaact gttcgtgttg gtggggctgc ctgctgccgc aagtgtgatc aactttgtgg
     4981 tactgacctc tgcggcgtct tccgctaaca gcggcgtctt ctctaccagc cgtatgctgt
     5041 ttggtctggc gcaggaaggt gtggcaccga aagcgttcgc taaactttct aagcgcgcag
     5101 taccagcgaa agggctgacc ttctcctgca tctgcctgct cggcggtgtg gtgatgttgt
     5161 atgtgaatcc tagcgtgatt ggcgcgttca cgatgattac aaccgtttcc gcgattctgt
     5221 ttatgttcgt ctggacaatt atcctttgct cgtaccttgt gtatcgcaaa cagcgtcctc
     5281 atctgcatga gaagtcgatc tacaagatgc cgctcggcaa gctgatgtgc tgggtatgta
     5341 tggcgttctt tgtgttcgtg gtcgtgttgc tgacactgga agatgacact cgccaggcgc
     5401 tgctggtcac tccgctgtgg tttatcgcgc tggggctggg ctggttgttt attggtaaga
     5461 agcgggctgc tgaactgcgg aaataaccgc attatcatgc tggatggcgc aatgccatcc
     5521 agcttttaga tcactcaccc gccagcgcgc gtgggaacag tacattgttt tccaggctga
     5581 tgtgatccat caggtcatca atcagttcat taatgccgtt atacatcgct ttccaggtgg
     5641 tgcaggcttc cggcggcggt gtgacgttat tggtggtgtg tttgatcact tccagcagtt
     5701 cgcccgcttc atcgtgctcg ctttccatta cgctgattgg ccccattgcc tggctgccca
     5761 tgccttgttt gatcatcggg aagaggatct gctcttcttt catcatgtgg ctggaaagct
     5821 cttcatgcag catagtcagg tattttgtca gcccttttgg cacgctcggt ttgtcggcgt
     5881 gaacgcgctc gactttagtc gcttgcagaa tcagctccgg cagttgctcg cggtgacgat
     5941 cgtggtagcg cacgatgata tggtcgatga tttctgccag cggggcgcta cgccagtctt
     6001 tctcaatcgg ttgttcagcg agctttgcca gttcagcttc aatgacctca acatccagtt
     6061 ctttacgtgc cgccgcacgc gccagcgttt gcttaccgcc gcagcagtaa tccatatcat
     6121 atttacgaaa cagagctgaa gcgcgaggaa tagagagcgc cagttcacct aaaggttggt
     6181 cgcgataagc catagctgat acct
//
LOCUS       DAZFRG010000261         6200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFRG010000261 DAZFRG010000000
VERSION     DAZFRG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.263"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..970)
                     /gene="fmt"
                     /locus_tag="ACHKUC_004244"
     CDS             complement(23..970)
                     /gene="fmt"
                     /locus_tag="ACHKUC_004244"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO6620387.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(985..1494)
                     /gene="def"
                     /locus_tag="ACHKUC_004245"
     CDS             complement(985..1494)
                     /gene="def"
                     /locus_tag="ACHKUC_004245"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO6620388.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            1624..2748
                     /gene="dprA"
                     /locus_tag="ACHKUC_004246"
     CDS             1624..2748
                     /gene="dprA"
                     /locus_tag="ACHKUC_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6620389.1"
                     /translation="MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            2720..3193
                     /gene="smg"
                     /locus_tag="ACHKUC_004247"
     CDS             2720..3193
                     /gene="smg"
                     /locus_tag="ACHKUC_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO6620390.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            3222..3764
                     /locus_tag="ACHKUC_004248"
     CDS             3222..3764
                     /locus_tag="ACHKUC_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO6620391.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            3769..4341
                     /gene="tsaC"
                     /locus_tag="ACHKUC_004249"
     CDS             3769..4341
                     /gene="tsaC"
                     /locus_tag="ACHKUC_004249"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO6620392.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            4346..5164
                     /gene="aroE"
                     /locus_tag="ACHKUC_004250"
     CDS             4346..5164
                     /gene="aroE"
                     /locus_tag="ACHKUC_004250"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO6620393.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            5161..5418
                     /locus_tag="ACHKUC_004251"
     CDS             5161..5418
                     /locus_tag="ACHKUC_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO6620394.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(5394..5948)
                     /locus_tag="ACHKUC_004252"
     CDS             complement(5394..5948)
                     /locus_tag="ACHKUC_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO6620395.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(5992..>6200)
                     /locus_tag="ACHKUC_004253"
     CDS             complement(5992..>6200)
                     /locus_tag="ACHKUC_004253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620396.1"
                     /translation="QDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCLLL
                     FHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         1555 a         1469 c         1556 g         1620 t
ORIGIN      
        1 ccgggcttag aagagtggac tatcaggcca gacggttgcc cggtacaaac cattcccgac
       61 gagaattcag gaggtcttgc acgctcatcg ctttcttacc tgcaggttgc attgagagca
      121 gattcaggat gccatcacct gtcgctacct gaatgccttg tttattggct tcaaggattg
      181 ttcctggcgc agcctttgtt gtcgtatcga tgacagatgc tttccagact ttaaccggct
      241 gcccttcaat ttccagccag ctcattggcc atggattgaa agcgcgaatg caacgttcaa
      301 gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac ttttcggcgt
      361 aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc agttgtttca
      421 acgtagtaat aagcccttgt gggccaagct ctgccagttt gtcgtacagc gtaccactgg
      481 tatcttctgc agtaatcggg caggagagct tataaagcat gtcaccggtg tctaaaccaa
      541 catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt gagcgttgga
      601 ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata cagccaagac
      661 gcggcatctc cagcacagct ttaggcagaa ttaaaccata ggcgacgacg accataacat
      721 cagcctgcag atcggcgacc agttgctggt tttcttgtgg acgcagggaa acaggttgaa
      781 aaacgggcag acctttgtcc tcagccagaa ctttaaccgg gctgggcatc agttttttac
      841 cgcgtcctgc cggtcggtct ggctgggtaa acacgccaac gatgttatga ccagaagaca
      901 acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata atacgtagtg
      961 attctgacac gttaattctt atccttaagc ccgggctttc agacgatcca gtttttcaac
     1021 tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca gtttgccgac
     1081 caggtgatcc atctcatgct gaatacagat ggctaacaga ccgtctgctt ccagttcaaa
     1141 tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg gcactaaagc
     1201 acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc ttttttctaa
     1261 aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat caattacaat
     1321 gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt ctgcgtacat
     1381 cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt ctaccggttt
     1441 agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg acataaatct
     1501 ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa atccccctct
     1561 gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg cagggagata
     1621 aggatggtcg atacagaaat ttggctgcgt ttaattagca tcagcagctt gtatggcgat
     1681 gatatggtcc gtatagctca ctggctggca aaacagtcgc atattgatgc ggttgtattg
     1741 cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc gcgaaagagt
     1801 atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc tgcggacagc
     1861 gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact gtttgttgaa
     1921 ggagaactgc acgcgttgca ttcatttcag cttgccgtag tggggagtcg ggcgcattca
     1981 tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgacgcg tggagtgaca
     2041 attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc cttacaggta
     2101 aatggcgtta gcattgctgt attggggaat ggacttaata ccattcatcc ccgccgccat
     2161 gctccactgg ctgccagtct acttgagcaa ggtggtgctc tcgtctcgga atttcccctc
     2221 gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag tggtctaagt
     2281 aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac agcacgttgt
     2341 gcgcttgagc aggggcgtga agtttttgct ttgccaggtc caatagggaa tccgggaagc
     2401 gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc ggaagaaatt
     2461 ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa ttcattttat
     2521 tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa cgtaggagat
     2581 gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc agaggtagtt
     2641 actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg cggctatgtc
     2701 cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa cctatattca
     2761 cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta ccgacgcagg
     2821 atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg cggattatca
     2881 ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc gtatttatac
     2941 ccctgaagag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt tccttgagca
     3001 aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc tcgcgctgga
     3061 taccgctgag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt tgttcaatat
     3121 tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag tgaatgaagg
     3181 tatgctgcat taatcatttc tttaattcag cataagtagt tatggcgaaa tcagcactgt
     3241 tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactagtt attcgatccg
     3301 ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac gtccgtcctc
     3361 tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt tgccctgcat
     3421 gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt tgcagtaact
     3481 accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca attacatgcc
     3541 cccaatgccg gacgggccat ctggttcagc gccgctcccg ttatggcaaa acatttcact
     3601 cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata gctggagaat
     3661 gccctgagtg ccattatccg ctactcatcg aaaagaaaac cgcgcagggt gtaaaacact
     3721 tttgtgccag taaacaatgt ggaaagccga tttcggcgga ataataacgt gaataataac
     3781 ctgcaaggag acgctatcgc agctgcgata gatgtcctca atgaagaacg tgtcatcgcc
     3841 tatccaacgg aagccgtttt cggtgtcggg tgcgatcctg atagcgaaac agcagtgatg
     3901 cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattctaat cgccgcaaat
     3961 tacgagcagc ttaaacccta tattgatgac accatgctga ctgacgcgca gcgtgaaact
     4021 atattttctc gctggccagg tcctgtcacc tttgtttttc ccgcgcctgc gacaacaccg
     4081 cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca tccgttggtg
     4141 gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa cttgagtgga
     4201 ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc gttcccggtt
     4261 gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc cctgacgggt
     4321 gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta atccgatagc
     4381 ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata ttgaacatcc
     4441 ctatggacgc gtgttggcac ccatcaatga tttcatcaac acactgaacg ctttctttag
     4501 tgctggtggt aaaggtgcga atgtgacggt accttttaaa gaagaggctt ttgccagagc
     4561 ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca agcggttaga
     4621 agatggacgc ctgctgggtg acaataccga tggtataggt ttgttaagcg atctggaacg
     4681 tctgtctttt atccgtcctg gtttacgtat tctgctcatt ggcgctggtg gagcatctcg
     4741 cggcgtacta ctgccactcc tttccctgga ctgtgcggtg acaatcacta atcggacggt
     4801 atctcgcgca gaagagttga ctaaattgtt tgcgcacact ggcagtattc aggcgttggg
     4861 tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat ccagtggcat
     4921 cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt attgctatga
     4981 catgttctat cagaaaggaa aaactccttt tctggcatgg tgtgagcagc gaggctcaaa
     5041 gcgtaatgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct tccttctttg
     5101 gcatggcgtt ctgcctgatg tagaaccggt catcaaacta ttgcaacagg aattgtcagc
     5161 gtgaatcagg ccatccagtt tccggatagg gaagagtggg acgagaataa aagatgtgta
     5221 tgttttcccg ctctcgtgaa tggtatgcaa ctgacatgtg cgatctctgg cgagagtctg
     5281 gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg tcagcatcgc
     5341 tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga tgatcaaggt
     5401 tgggtctggt taccttgatc cagatattcg tccttccatt tcacgtagtt atttgcggaa
     5461 tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac ggggctaccg
     5521 agatacagat atccgctctc cagccgttta ttttgtggga ccaggctacc cgcaccaatc
     5581 atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc aaccaaaact
     5641 cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac atcttcgcca
     5701 atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg caacatactg
     5761 ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc acgaatcaca
     5821 acgagcggcc agatccccac atcatcagcc aaacgaacgt caccaatcac gacgctgcta
     5881 tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg gcgtaaaaca
     5941 tcagacatac tcacctcaac aataaatgat ttactaatga ctttgggtgc attattggcc
     6001 ttgtgcaagt cttttagcat gcaaaaaagc accgttttgt gtgcgaatgc agcaaaaagg
     6061 gtgaaaaagc aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa aattgggatc
     6121 cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg gtcgtaacaa
     6181 caaagagaaa aaatcctgaa
//
LOCUS       DAZFRG010000262         6163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFRG010000262 DAZFRG010000000
VERSION     DAZFRG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.264"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1311
                     /gene="alsA"
                     /locus_tag="ACHKUC_004254"
     CDS             <1..1311
                     /gene="alsA"
                     /locus_tag="ACHKUC_004254"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO6620397.1"
                     /translation="KLAAQLGIGIIYQELSVIDELTVLENLYIGRHLTKKICGVNIID
                     WREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTLMLDAKVIIMDEPTSSL
                     TNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVMKDGSSVCSGMVSDVSN
                     DDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDRKKVRDISFSVCRGEIL
                     GFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLDAVKKGMAYITESRRDN
                     GFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQRELLALKCHSVNQNIT
                     ELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYKVMRQLADDGKVILMVS
                     SELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALPHE"
     gene            1290..2270
                     /gene="alsC"
                     /locus_tag="ACHKUC_004255"
     CDS             1290..2270
                     /gene="alsC"
                     /locus_tag="ACHKUC_004255"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO6620398.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            2281..2976
                     /gene="alsE"
                     /locus_tag="ACHKUC_004256"
     CDS             2281..2976
                     /gene="alsE"
                     /locus_tag="ACHKUC_004256"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO6620399.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            2960..3889
                     /gene="alsK"
                     /locus_tag="ACHKUC_004257"
     CDS             2960..3889
                     /gene="alsK"
                     /locus_tag="ACHKUC_004257"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO6620400.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            4162..6147
                     /gene="yjcS"
                     /locus_tag="ACHKUC_004258"
     CDS             4162..6147
                     /gene="yjcS"
                     /locus_tag="ACHKUC_004258"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO6620401.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIIAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHTVLTGQAKMT
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         1554 a         1548 c         1576 g         1485 t
ORIGIN      
        1 aaattagcgg cacaactcgg tatcgggatt atttatcagg aactcagcgt tattgatgaa
       61 ttaaccgtac tggaaaattt atatattggt cgtcatctga cgaaaaaaat ctgtggcgtc
      121 aatattatcg actggcgaga aatgcgtgtc cgcgccgcca tgatgttatt acgcgtgggc
      181 ttgaaagttg atttagatga gaaagtggcg aatttatcta tcagccacaa gcagatgctg
      241 gaaattgcca aaacgctgat gctcgacgcc aaagtcatca tcatggatga acccacctcc
      301 tcactcacca ataaagaggt ggactatctg tttctgatca tgaatcagtt gcgtaaggag
      361 ggtacggcca tcgtctatat ctcgcataaa ttggcggaaa ttcgccgtat ttgcgaccgc
      421 tatacggtga tgaaagacgg cagcagcgtt tgcagcggca tggtgagcga tgtgtcaaat
      481 gacgatatcg tccgtctgat ggtaggccgc gaattgcaaa accgttttaa cgcgatgaag
      541 gagaatgtca gcaaccttgc gcacgatacg gtttttgagg tgcgcaacgt caccagccgt
      601 gacagaaaaa aggtccggga tatctcattt agcgtctgcc ggggagaaat attaggcttt
      661 gccggactgg tcggttccgg acgtactgaa ctgatgaact gcctgtttgg cgttgataaa
      721 cgcgctggcg gagaaatccg tcttaatggc aaagatatct ctccacgttc tcccctggat
      781 gccgtgaaaa aagggatggc ttacatcact gaaagccgcc gggataacgg ctttttcccc
      841 aacttttcta tcgctcagaa catagctatc agccgcagtc tgaaagacgg cggctataaa
      901 ggcgcgatgg ggttgtttca tgaagttgac gagcaacgta ccgctgaagc ccagcgcgaa
      961 ctgctggcgc tgaaatgtca ttcggtaaac cagaacatca ctgaactctc cggcggtaac
     1021 cagcagaaag tcctgatctc caaatggctg tgctgtcacc cggaagtgat cattttcgat
     1081 gaacctaccc gtggcatcga cgttggcgcg aaagccgaaa tttacaaagt gatgcgccaa
     1141 ctggcggacg acggaaaagt catcctgatg gtgtcatctg aactacctga aattatcacc
     1201 gtctgcgacc gcatcgccgt gttctgcgaa ggacgactga cgcaaatcct gaccaatcgc
     1261 gatgacatga gcgaagagga gattatggca tgggctttac cacacgagta aaaagcgaag
     1321 cgagcgagaa gaaaccgttc aactttgcgc tgttctggga taaatacggc acctttttta
     1381 tcctggcgat catcgtcgcc atttttggtt cgctgtcgtc agaatatttt ctgaccacca
     1441 ataatattac ccagattttt gtgcaaagct ccgtgacggt actgatcggc atgggggaat
     1501 tcttcgctat cctggtcgcc ggtatcgacc tttcggttgg cgcgattctg gcgctttccg
     1561 gtatggtgac cgctaaactg atgttggcag gtattgatcc gtttctcgcg gcgctaattg
     1621 gcggtgtact ggttggcggc gcactggggg cgatcaacgg ctgcctggtc aactggacgg
     1681 gactacaccc gttcattatc acccttggca ccaacgctat tttccgtggg atcacgctgg
     1741 tgatctccga tgccaactcg gtatacggct tctcatttga cttcgtgaac ttctttgccg
     1801 ccagcgtaat tgggatacct gtccccgtta tcttctcgct aattgtcgcg ctcatccttt
     1861 ggtttctgac aacgcgtatg cggctcggac gcaacatcta cgcactgggc ggcaacaaaa
     1921 actcggcgtt ctattccggg attgacgtga aattccacat cctggtggtg tttatcatct
     1981 ccggtgtttg tgcaggtctg gcaggcgtcg tctcaactgc acgactcggt gccgcagaac
     2041 cgcttgccgg tatgggtttt gaaacctatg ccattgccag cgccatcatt ggcggcacca
     2101 gtttcttcgg cggcaagggg cgcattttct ctgtggtgat tggcgggttg atcatcggca
     2161 ccatcaacaa cggtctgaat attttgcagg tacaaaccta ttaccaactg gtggtgatgg
     2221 gcggattaat tatcgcggct gtcgcccttg accgtcttat cagtaagtaa ggaattgaac
     2281 atgaaaatct ccccctcgtt aatgtgtatg gatctgctga aatttaaaga acagatcgaa
     2341 tttatcgaca gccatgccga ttactttcat atcgatatca tggacggcca ctttgtgccg
     2401 aatctgacgc tctcaccgtt cttcgtaagc caggtaaaaa aactggcgag taaaccgctc
     2461 gactgccatc tgatggtaac gcgtccgcag gattacattg ctcaactggc acgtgcggga
     2521 gctgatttca tcactttgca tccggaaact atcaacggtc aggcattccg cctgattgat
     2581 gaaatccgcc gtcatggcat gaaagtgggg ctgattctta atccggaaac gccggttgag
     2641 gccatgaaat actatatcca taaggctgat aaaattacgg tcatgactgt cgatcccggt
     2701 tttgccggac aacccttcat tcctgaaatg ctggataaag ttgccgaact gaaggcatgg
     2761 cgtgaacgag aaggtctgga gtacgaaatt gaggtggacg gttcctgcaa ccaggcaact
     2821 tacgaaaaac taatggcggc aggggcggat gtctttatcg tcggcacttc cggcctgttt
     2881 aatcatgcgg aaaatatcga cgaagcatgg agaattatga ccgcgcagat tctggctgca
     2941 aaaagcgagg tacagcctca tgcaaaaaca gcataacgtc gtagcgggcg tggatatggg
     3001 ggcaacgcat atccgctttt gtctgcggac agcagaaggt gaaacgctac actgcgaaaa
     3061 aaagcggacc gcagaagtta ttgctcccga cctggtgtcg ggtatcggcg aaatgattga
     3121 cgagcaactc aggcgcttta acgctcgctg tcatggtctg gtgatgggat ttccggcgct
     3181 ggtcagtaaa gataaacgca ccattatttc tacgcctaac ctgccgttaa cagcggcaga
     3241 tttatatgat ctcgccgata agctcgaaaa tacgctgaat tgtccggttg agttttcccg
     3301 cgacgttaac ctgcaactct cctgggacgt agtagaaaac cgccttacgc aacaactggt
     3361 tctggcagcc tatctcggta cggggatggg gttcgcagtg tggatgaacg gtgcgccgtg
     3421 gacgggtgca cacggtgtgg caggcgaact gggccatatc cccctgggag atatgaccca
     3481 acactgcgcg tgtggcaatt ctgggtgcct ggaaaccaat tgctctggaa tggcgctaag
     3541 acgctggtac gaacaacagc cccgaaatta cccattgagc gatcttttcg tccatgcgga
     3601 aaacgcccct ttcgtccaga gtctgcttga aaacgcggca cgagccattg ccaccagcat
     3661 taatctgttc gatcccgatg cggtgattct gggcggtggc gtgatggata tgcccgcctt
     3721 cccacgcgag actctcattg ccatgaccca aaagtacctg cgccgtccac tgccgtatca
     3781 ggttgtgcgc tttattgccg cctcatcttc tgactttaat ggcgctcagg gtgcagcaat
     3841 attggcgcat caacgttttt tgccacagtc ctgtgctaaa gtcccatgag taaagcgttg
     3901 gcacagtagc gcaatgtccc gctgtgccaa cgacaggtac aatatatata ataattacat
     3961 aaaacagccc atattattcc cagcaatgat agttctactg taattttccc tcttcagcac
     4021 aaatgcctaa taaaatggct cgttccgctc acacttccac ttattaaagt cgcaatttca
     4081 gaattatgat aacgccagct ttaaatgacg tattttgcat ggattacata tttacgacat
     4141 aaatgataaa tggagattta tatgaataac tctcggttat tccgtttgag caggattgtt
     4201 attgcgttca ctgccgccag cggcatgatg ataaataccg cttacgcaac agatgaagcg
     4261 aaagccgcca ctcaatatac ccaacaggtt aatcagaatt acgccaaatc attaccgttt
     4321 agcgatcgtc aggattttga cgatgcccag cgtggattta tcgctccgct gctggatgaa
     4381 ggtattctgc gtgatgcgaa cggtaaaatt tactaccgcg cgaatgatta caaatttgat
     4441 attaatgccg ctgcgccgga aaccgtaaac cccagcctgt ggcgtcagtc gcagatcaac
     4501 ggcatttcag gcctgttcaa agtcaccgat aaaatgtatc aggtgcgcgg ccaggatatc
     4561 tctaacatta cgttcgttga gggcgagaaa ggcattattg ttattgaccc actggtgacg
     4621 ccacctgccg caaaagccgc acttgacctt tacttccagc atcgcccgca aaaacccatt
     4681 attgccgtta tctataccca cagccacgcc gaccactatg gcggcgtgaa aggcatcatc
     4741 tctgaagccg atgttaaatc cggaaaagtt caggtgattg ctccagctgg ctttatggac
     4801 gaagccatca gcgaaaacgt gctggcgggt aacatcatga gccgccgtgc gctctactct
     4861 tacggtctgt tactgccgca caacgcgcaa ggcaatgtgg gtaatggcct tggcgtgacg
     4921 ctggcaacgg gcgacccgag cattattgca ccgactaaaa ctatcgtcag aactggcgag
     4981 aagatgatta tcgacggcct ggagtttgac ttcctgatga ccccaggtag cgaagcgcca
     5041 gccgaaatgc acttctatat tccggccctg aaagccctgt gtaccgccga gaacgccacg
     5101 cataccctgc acaacttcta cactctgcgc ggcgcgaaaa cccgcgacac cagcaagtgg
     5161 accgagtatc tgaacgaaac gttggatatg tggggtaacg acgcggaagt actgtttatg
     5221 ccgcacacct ggccggtctg gggcaataaa catatcaatg attatattgg taaatatcgc
     5281 gatactatca agtacattca cgaccagacc ctgcacctgg cgaaccaggg ctacaccatg
     5341 aatgaaatcg gcgacatgat taagctgccg cctgcgcttg ccaataactg ggccagccgc
     5401 ggctattacg gttctgtcag ccacaacgcc cgcgcggtat ataacttcta tcttggctat
     5461 tacgacggta acccggctaa cctgcatccg tatggtcagg tggagatggg taaacgttac
     5521 gtgcaggcgc tgggcggttc tgcccgcgtc atcaacctgg cgcaagaagc gaacaagcaa
     5581 ggtgattatc gctggtcggc agaactgctg aaacaggtga ttgccgccaa cccgggtgac
     5641 caggtcgcga agaatctgca agcgaataac tttgaacagc tgggctatca ggccgagtcc
     5701 gccacatggc gcggtttcta cctgaccggc gcgaaagagc tgcgcgaagg ggtgcataag
     5761 ttcagccacg gcaccaccgg ttccccggac accattcgcg ggatgtcggt cgaaatgctg
     5821 ttcgacttta tggccgttcg cctcgatagc acgaaagccg caggtaaaaa tatcagcctg
     5881 aacttcaata tgagcaacgg cgataacctt aacctgatgc tgaacgatag cgtgcttaac
     5941 taccgtaaaa cgctgcaatc gcaagccgac gcctctttct acattagccg tgaagatctg
     6001 cacaccgtgc tgaccggaca ggctaaaatg accgatctgg tgaaagcgaa gaaagccaaa
     6061 attattggca atggcgcgaa actggaagaa attatcgcct gtctggataa tttcgatttg
     6121 tgggtgaata tcgtaacccc aaattaatgt cactcgcttt tgg
//
LOCUS       DAZFRG010000263         6136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFRG010000263 DAZFRG010000000
VERSION     DAZFRG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.265"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1428
                     /locus_tag="ACHKUC_004259"
     CDS             <1..1428
                     /locus_tag="ACHKUC_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6620402.1"
                     /translation="FTMTLLSSSLAGLLVMSGGVSAQEEKYTVQYAIGKYGSTYEVVK
                     TGDNGNFRYEVKEKNGKRSLFTFDSEGNVTVKGKGITHTLHDPALKDFAKAVEGKTDH
                     KLINSDVRGVYNKVYGLEKTEITGFSVEDGENGKVSLGSDAKVSGKFSVAVGNGAKAT
                     EKASTSVGSWSAALGQQSVALGVGAYAYANASTAAGTAAYVDGSAIYGTAIGNYAKVD
                     KNATEGTALGAKATVTNKNSVALGANSRTTRDNEVYIGYEAEPGKAYKTRVLGGLSDG
                     TRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEAKKSREYTDSRTTVGVNSDGTLTRA
                     EGASKTLAVNDGLVALSGRTDRIDAAVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQ
                     QRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNE
                     QTAAKAGIAASDGDVSYNMGVNFEF"
     gene            1517..1987
                     /locus_tag="ACHKUC_004260"
     CDS             1517..1987
                     /locus_tag="ACHKUC_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620403.1"
                     /translation="MKVILATRNRYLEYGLQALLKGHSVILAREFFLPENRRYIPDFD
                     ESWLIISDGLLGRLMRCMFQGRHFLQLDAELLRDGEQISDAIHNGVWTYNSAARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGINTKTVNTFLYTGMAKNGLYGVSVRRLVGA"
     gene            2350..3975
                     /locus_tag="ACHKUC_004261"
     CDS             2350..3975
                     /locus_tag="ACHKUC_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620404.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGGGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNAALATEQIISPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFNPAGMDIRYLSTPFDCACVDLVNDVMRVVSGQNMSAIAGGS
                     LPSTIRWHSKVFSLPERTSFSCLRVKSPTPERVGITVLADDVPVIHLAPGSFSGSVVR
                     LPAATGQNWQILVSGFGQVERITLSTSMSELPV"
     gene            3972..5240
                     /locus_tag="ACHKUC_004262"
     CDS             3972..5240
                     /locus_tag="ACHKUC_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6620405.1"
                     /translation="MTKKPWRAGKDLSAVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGAAYALNPGIEMDSSVMVVDFPSKPQNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWHAVAGVA
                     AQTQISVQAVIDQIKEEAAKSPVIEELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLS"
     gene            5255..5536
                     /locus_tag="ACHKUC_004263"
     CDS             5255..5536
                     /locus_tag="ACHKUC_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620406.1"
                     /translation="MAEYGFAIYNRNNVNVTGVLTPIFFLDRFTAESGSKTYTNKPEG
                     KTLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            5542..>6136
                     /locus_tag="ACHKUC_004264"
     CDS             5542..>6136
                     /locus_tag="ACHKUC_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620407.1"
                     /translation="MYGLSITKPDGSLWISPGFTPQCLINKGTIPATEKAFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHQIVRGTNPGVTTVYAFANMVTQPSEY
                     GIAMYNPSGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLV"
BASE COUNT         1575 a         1283 c         1755 g         1523 t
ORIGIN      
        1 tttacaatga cactcctgtc atcttctctg gccgggcttc tggtgatgag tggtggggtg
       61 agtgcgcagg aagaaaaata cacagtgcag tatgccatag gtaaatatgg cagcacctat
      121 gaagttgtaa agacaggcga taatggtaac ttcagatacg aagtaaaaga gaaaaacggc
      181 aagaggtcac ttttcacatt tgattcagaa ggtaatgtga ccgttaaagg taagggtatc
      241 acccatacac ttcatgaccc ggcgctgaaa gattttgcaa aggctgttga gggtaaaaca
      301 gatcacaagc tgataaacag tgatgttcgt ggcgtgtaca acaaggtgta tggcctggaa
      361 aaaacagaaa ttacaggttt cagcgtcgaa gatggggaga atgggaaggt ttctctgggt
      421 agcgatgcga aagtctctgg taagttctca gtagctgttg gtaacggtgc taaggctaca
      481 gaaaaagcgt caacatcagt gggatcatgg tctgcagcac taggacagca aagtgtggct
      541 ttaggtgtgg gagcatatgc ctatgcaaat gcttcaacag ccgccggaac agctgcttat
      601 gttgatggtt ctgctattta tggtaccgct ataggtaatt acgcaaaagt ggataaaaat
      661 gcaacagaag gtactgcgtt aggggcaaag gcaacagtaa caaataaaaa cagtgttgca
      721 ttaggagcta attcacgcac aacccgtgac aatgaggttt atatcggtta tgaagcagag
      781 ccgggtaagg cttataaaac ccgggtgctt ggtggtctga gtgacggtac tcgtccctca
      841 gatgctgcaa ccgttcgtca ggttgaccgt gtaaaagaca gtgttgagca actggctcag
      901 gatacgaata cccgccttgt ggttgaagcg aaaaagtctc gtgaatatac agactccaga
      961 acgacagttg gtgttaactc cgacggaacc ctgacccgtg cagaaggagc cagcaaaacc
     1021 ctcgctgtta atgacggtct agttgctttg tccggcagaa cagaccgtat tgatgctgca
     1081 gttggtagcg ttgaccgccg ggttactaaa aatacacagg ctattcagtc caatactcgc
     1141 cagttgcagg agcataatgc acgcctgaac agtcagcagc gtcaaattcg tgagaatcac
     1201 gaagagatga agcgtgcagc agcacagagt gcagcgctgg cggggctttt ccagccgtac
     1261 agtgtgggga aattcaacgc cactgctgcc ctgggggggt acagtgataa acaggctgtt
     1321 gccgtgggtg ttggttaccg tttcaacgag cagaccgcag cgaaagcggg tattgcagcg
     1381 agtgatggcg atgtgtccta caacatgggc gttaacttcg agttttaatt gctgaaagtg
     1441 acatgaagta tggcggcagg cgcaggactc tggtctgccg tttttcatgg ggcataaaaa
     1501 acaggaaggt gctgcgatga aggtgattct ggctaccagg aaccgctatc tggagtatgg
     1561 tctgcaggcg ttactgaagg ggcacagtgt gatactggcg agggagtttt tcctgccgga
     1621 gaaccgccgt tatatcccgg actttgacga atcctggctg ataatcagtg atggcctgct
     1681 gggcaggctg atgcgctgta tgttccaggg gcgtcatttt ctgcaactgg atgcggagtt
     1741 gctccgtgat ggtgagcaga taagtgatgc catacacaac ggcgtctgga cgtataacag
     1801 tgctgcccgc ccgctgacga tgtcagagat ggtggtgatg ttcggttatg tctaccgcca
     1861 gtcgagaccc tgccgccttg ccagtgagat gggtattaac acaaagacgg tgaatacctt
     1921 tctgtatacg ggaatggcga aaaacgggct gtatggtgtg agcgtcagac ggcttgttgg
     1981 tgcctgatgg cggtcctgcc ggatgcgcgg catcttctga tgattcagaa ggtgcagatg
     2041 atgaacaaag acagagaccg gagatacggg agaaagcgtt acaccacagg cgcaatgtgt
     2101 ttcatgttgt ttgtgtggct tggtggtgtg ctggctcttg ttgctacagc tgcggtggta
     2161 atgcagtaaa aaaaggggag caacatgctc cccgaccaga agaaagacgt ttgataacaa
     2221 ttagtgagtt gttacgtctt gtccagaata tcatagcaac actctgttgc agtgataccg
     2281 atcgcgattt tagcgaattc catcataaat cccctgattt ttaagcctga agcagtcaaa
     2341 ggaatttcta tgccctatat cgatatcacc acgatgcgtg ggatgatgcc gcgcgttgtg
     2401 acatccatgc tgcccgagca ttccgctgta ctggcggagg actgccattt ccggtttggt
     2461 gttattacac cagaacgtca gatatccggg gttgagaaaa cattcacaat taagccaaaa
     2521 acaatttttc attaccgtga cgatttctgg tttgcatggc cggatgtggt ggatgtgatc
     2581 cgcagtccga tcgctcagga cccccacggg cgtatttact acactgacgg gcgttttcct
     2641 aaagtgacgg atgcgaccat tgccacaaaa gggggcggga atcacccgac atcatcgtat
     2701 cgtctgggga tccccgcgcc gacgacagct cctgtctgta ctgttcagca gggcggtgat
     2761 gtttctgacg ataacccgaa tgatgatgaa acccggtttt atacggaaac ctttgtctca
     2821 gattatggtg aagaaggtcc gccaggtccg gcgtctctgg aggtaacact ccgtactccg
     2881 ggaactgcgg tacaactgac gctggctccg gtgccattgc agaatgccag tattaaacgt
     2941 cgtcgtattt atcgttctgc ctcaggggga ggagaagccg attttttact cgtggctgaa
     3001 ctggatgcat ccgtgctcag ttacacggat aaaataccgg cgaaaaatct tggaccttcg
     3061 ctggcgacat gggattacct gccgccgccg gagaatatga caggcctttg cctgatggcg
     3121 aatggtattg ccgccggatt tgccggtaat gaagtgatgt tttcggaagc gtatctgccg
     3181 tatgcatggc cggaagtgaa tcgtcacacg acggcagaag atattgtggc tatctgtccg
     3241 ctcggaacat cactggtggt ggcgacaaag ggtgagcctt atctgttcag tggtgtatcg
     3301 ccttccacaa tttctggctc cagaattcct tcaatgcagg cgtgtctgag caggcggagt
     3361 atggtcgcga tggagggctt tgtgctgtat gcaggaacaa atggcctggt gtctgttgat
     3421 gcaaacggta atgccgctct ggcgacggag cagattattt cgccggaaca gtggcagagt
     3481 cagtttaatc cggcctccat tgtggcttat ccctggcgtg gtgaatacat tgcctgttac
     3541 acgaaaccgg atggtaagca ggatgtgttt gtcttcaacc cggcaggtat ggatatccgc
     3601 tacctcagta caccttttga ctgcgcatgt gttgacctgg ttaacgatgt tatgcgggtg
     3661 gtatcaggac aaaacatgtc tgcgatcgcc ggagggagtt tgccgtcgac aatcagatgg
     3721 cattcaaagg tattttccct tccggaaaga acctctttct cctgtctcag ggtgaagtct
     3781 ccgacgccag agcgggtggg aattactgtg ctggcagatg atgttccggt gattcacctt
     3841 gcacctggaa gcttttcggg aagcgttgtg agattacctg ctgcaaccgg gcaaaactgg
     3901 cagatactgg tttccggttt tggtcaggtt gaacgtatta cactcagtac atcgatgtcg
     3961 gagctgccgg tatgacaaaa aaaccgtggc gcgcaggaaa ggatttatct gcggttgtgg
     4021 aaaatatgga gatagggaca ggacaacgtg gcgatggtcg tcatgctttt gtaacccgtg
     4081 aagaactggt cggtctgaag ctggcccgtc gccggacttc aggtggtgcc gcatatgccc
     4141 ttaatccggg gattgagatg gacagctctg taatggtcgt tgatttccca tcgaaacctc
     4201 agaattttaa ggcgacaggg ggatttggct cggtactgct tgaatgggat atgccgaatt
     4261 atcgcggtca ttcactgact gaaatctggc ggggtacgga ggatgacctt gctgatgccg
     4321 tgctggttgc cacgacgcct gggcaggtct acggcgatcc ggttgatccg ggatggtcgg
     4381 gattttactg gatcaggttt gtgaatgccg caggcgttaa agggccgtgg cacgcagtgg
     4441 caggcgtggc agcacagaca cagatatcag ttcaggcggt gattgaccag ataaaagaag
     4501 aggcggcgaa gtctcccgtc atagaagagc ttcgcaagga aataaaaaac gcgcaggggc
     4561 aggctgtaaa ggatgctgca attaagacaa ccgaagttgt ggggactctc agggaagaaa
     4621 cgacaagaac gattggtggt attgaaaccc gcattagcac actggattcg tcaaccagtg
     4681 aatcgcttaa tgaggtcgac aagcgcatca ctaaactgga taaagaaggc ggtgaggcct
     4741 tccttgcgat gtggtcaaaa aaagcgggag ttgatggcat cactgcgggg atcgggattg
     4801 tcgccggaaa agacagtgaa ggcaggcctg taagtcaggt tgcaatttct gcgtcgcagc
     4861 tgtttgtctt tgacccgaac aacccggata acaccgccta tccgtttgcg gtatcaggtg
     4921 gcaaggtcgt gatcccgaaa gcgatgattt atgacgcggt gattgaaaca ctggtgtcgc
     4981 ggaaggttgt ggcagatgaa gtaaaagccg gggtaagtat cacttcgcca gttatccgga
     5041 gtgccgttat tcagaacgga aactttcagg ttgattctca gggtaacctg aatattggtg
     5101 gcctttttag tgttacgtcg caggggcaac tgacaattcg ttactctaat cagaatgtgg
     5161 ggctggtgat ccgcaatgat aaaattgaag tttatgacca gaacggacgc ctggctgttc
     5221 gcataggcag gttaagctga gccggaggct gacattggca gaatatggtt ttgcaatata
     5281 caacagaaat aatgttaatg ttacgggcgt gcttactccc atatttttcc tggacaggtt
     5341 tacggcggag tcgggatcaa aaacgtatac gaataaacca gaagggaaaa cattacaggc
     5401 agtctgttca ttatttccgt ggaataatgt atttaaggac cggaaagtac cgaagataac
     5461 cattaatggc aatacggtga catggtcgaa tcttgagcag gggatggggt catatattta
     5521 tacattctgg gggtgagttg tatgtatggt ttaagcatta cgaaaccaga tggcagcttg
     5581 tggataagcc cggggtttac gcctcagtgt ctgatcaata agggaactat tccggcgact
     5641 gagaaggctt tttttaaaac gtcaatcccg tcaggaaaaa gttgtttttt ctttatcaga
     5701 acagagaaaa aggccgatgt tatgtacacg catgaacaaa ttgatggata tcatgcctta
     5761 aggcttcatc aaattgtcag gggaacgaat cccggtgtta cgactgttta tgcttttgcg
     5821 aatatggtta ctcaaccttc tgagtatggt atcgccatgt ataacccgtc gggtgagatg
     5881 atttaccatg gtgaaatgat gctgcttgac gcgaagttaa taccagttga tatcaagttt
     5941 gagaaggacc ttggatatcc atgcgcaatt atgcctgcac tggttggata ttataactgg
     6001 aaacgaacac cttatgaccg accgatttac accacatcca ctggtgctac aggaaataaa
     6061 atatattcct gtgagcatta ttccggtggt gcaacatggg atatccgaaa gccgtatata
     6121 gataaagttc tggtta
//
LOCUS       DAZFRG010000264         6004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFRG010000264 DAZFRG010000000
VERSION     DAZFRG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.266"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..1632
                     /locus_tag="ACHKUC_004265"
     CDS             82..1632
                     /locus_tag="ACHKUC_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO6620408.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFSGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQMNASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1658..2110
                     /locus_tag="ACHKUC_004266"
     CDS             1658..2110
                     /locus_tag="ACHKUC_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO6620409.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFSLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2263..3537
                     /locus_tag="ACHKUC_004267"
     CDS             2263..3537
                     /locus_tag="ACHKUC_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6620410.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3530..5632
                     /gene="mchF"
                     /locus_tag="ACHKUC_004268"
     CDS             3530..5632
                     /gene="mchF"
                     /locus_tag="ACHKUC_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO6620411.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECARASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(5693..>6004)
                     /locus_tag="ACHKUC_004269"
     CDS             complement(5693..>6004)
                     /locus_tag="ACHKUC_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620412.1"
                     /translation="TTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADIAGSA
                     IGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSSSSSG
                     S"
BASE COUNT         1717 a         1146 c         1452 g         1689 t
ORIGIN      
        1 ataaggaaga agctgtcagg acacttactt gctcttttat gaacactctt ttatgaaaat
       61 tgaaacggag aatactgcca gatgagtcat cagtgttcac tttctgaact gaatgaaaac
      121 ctggtgcctt tcactgccag gcagatcaag gcctcattaa tctggtgtgc agaggatgtc
      181 agaaatccag acgagctgca aaatgcctgc agttatatta tcgatcctgg cagtacagct
      241 tctgccaaag tgttccatgc agagcgctat ggtggcagtg gtattcagcg taatggaggt
      301 ggtgcacgtt gtgggtttga tggtaactac caggttaaag gaataggaag taatccgttg
      361 gttggcgaag gtactgacga acgtcattct aatggtgcac tcggcgctgt tcatgcaata
      421 tatgaggctt tgtggggaga agtactggct caaatattac cttatagtgc tgtgcgggtt
      481 cgggcggttt tacttacaga tctctatact gaaaaggcat ttgagcgctc cggtaggaaa
      541 tcacgaagag ccctgttggt acgtgagcct gttgttcgtc cggcgcattt tgaacgggca
      601 ccttacttcc aagtaaaacc ggagtattcc agtctgttaa ttcacgatgc ctgtcgggtt
      661 agatctgtga tccacaagct gccaggatat ctacctgtac caccggaaga aattgatgct
      721 gaagcacgaa ctgatccccg gatttattgc attgaggggt tatgtgaact ggcacgtcgt
      781 gaggcctggc aaatggcatt ttgtcgaaca cgtttcctga gattgacaac ttctccttct
      841 aatattgcaa tggatggcag attaatggat tttagcggac tcagttgtct gtttccggga
      901 gattccccag ctgattttgg gtataaacta agattagctg aactggcaaa agaaccgatg
      961 gtacttatgc aagggctgtc tgatctctgc ttgtatatcg gaaaatatat gtttgaccct
     1021 gacttcactc ttgcagcccg tttgaaggtt gaggagatat ttcagaaaac ttttcatgaa
     1081 gcatgttatt actgttatct agaactgttg ggtattcctg gagaatttat aacacaaaaa
     1141 gagatacctg atatattgaa agaactggtt aacagttttg ttgcattact caatcaatac
     1201 tgcgagaaat cacatgccca agatattgtc aatcaggatg gttcaccatt gcaaaagttg
     1261 gttgtgacgc taatccatca taggcataat caaaagcagg cactgaatag tagcatcaag
     1321 aatgatgttt atttcaccgt tgcacaacag tgtttttccc aggccatcca ctggctgacg
     1381 caaggcagta ccagacgtca gatgaatgcc tcattactcc tgaaagaaat tgaacatcat
     1441 accatgaaaa ggctgcaacc cagggaagag ctgaggaaag agaatatgtg cgaaaaaatt
     1501 gccatcctgc tggataatca tggcgatgat cccctttttt tacaagaagc aatttctgat
     1561 atgaaaaatt ttatgcttaa gttttccaga gatgcatttg gatatcttga accgataaga
     1621 aacacagtgt aaaaaatata taagggaaat agtaatcatg tcttatataa gggaaaccat
     1681 cagaggaaaa gatgaatgga ctgtttatga acagatcggt tttgcggtca gttgtatgct
     1741 ctacaatcgt aattacagtc tgtatccggt gttaaccatt caatactgga ctgaatatgc
     1801 gatacagcat aatcagatta aattcctgtt tgattcacga ggttttccac tggcgtatat
     1861 tacctgggca tatcttgagg ctgatacaga agcgcgcctg ctcagggatc cagaattcag
     1921 cttgcatccg tctgaatgga atgaagatgg aaggatttgg attctggatt tctgttgtaa
     1981 accaggcttt ggtcgaaaag ttattgacta tctcatacag cttcagccat ggggagaagg
     2041 agaagtacga tggttaagca ggcgaaagaa aattgtgaca tacatccctg agcggctgca
     2101 taaaacgtag tacctctgaa gatacagaga taattgtaaa ttacggggta aatgcatcgc
     2161 tgatactatt ttgacaggac tctatatttt ctggctttga ttatagcatt tcggttaacc
     2221 agtgaaggta ttacaggaaa agtgtgagta agaggagcta ttttgtttcg tcaggatgct
     2281 ttagaaaaca gaaaaatgaa gtggcaggga cgggcaatat tacttcccgg aataccacta
     2341 tggttaatca tgctgggaag catagtgttt attacggcat ttctgatgtt cattattgtt
     2401 ggtacctata gccgccgtgt taatgtcagt ggtgaggtca caacctggcc aagagctgtc
     2461 aatatatatt caggtgtaca gggatttgtt gtcaggcagt ttgttcatga aggacagttg
     2521 ataaaaaaag gggatcctgt ttatctgatt gacatcagta aaagtacacg tagtggtatt
     2581 gtcactgata atcatcgtcg ggatatagaa aaccagttgg ttcgtgtgga caacattatt
     2641 tcccgtctgg aagaaagtaa aaaaataacg ctagataccc tggaaaaaca acgcctgcaa
     2701 tacacagatg cgtttcgtcg ctcatcagat attatacagc gtgcagagga agggataaaa
     2761 ataatgaaaa acaatatgga gaattacaga aactatcagg caaaagggct gattaataaa
     2821 gatcagttaa ctaaccaggt ggcattatat tatcagcaac aaaacaatct tctcagcctg
     2881 agcggacaga acgaacagaa tgccctgcag ataaccactc tggagagtca gattcagact
     2941 caggctgcag attttgataa ccgtatctac cagatggaac tgcaacggta tgagttacag
     3001 aaagaactgg ttaacactga tgtggagggc gaaatcatta tccgggcgtt gactgacggg
     3061 aaagttgact ccctgagtgt cactgtcggg caaatggtca ataccggaga cagccttctg
     3121 caggttattc ctgagaacat tgaaaactat tatcttattc tctgggtccc gaatgatgct
     3181 gttccttata tttcggctgg tgacaaagtg aatattcgtt atgaagcctt tccggcagaa
     3241 aaatttgggc agttctctgc tacggttaaa actatatcca ggactcctgc gtcaacacag
     3301 gaaatgttga cctataaggg agcaccacag aatacgccgg gcgactctgt tccctggtat
     3361 aaagtcattg cgatgcctga aaagcagatt atcagatatg acgaaaaata cctccctctg
     3421 gaaaatggaa tgaaagccga aagtacacta tttctggaaa aaaggcgtat ttaccagtgg
     3481 atgctttctc ctttctatga catgaaacac agtgcaacag gaccgctcaa tgactaacgg
     3541 gaatttcaga caaattataa atcagcttga tatgcgctgg cgacgtcgtg ttccggttat
     3601 tcatcagacg gagaccgctg aatgtggact ggcctgcctg gcaatgatat gcggtcattt
     3661 tggtaagaat attgacctga tatctcttcg ccggaagttt aatctctcgg cccgtggagc
     3721 aaaccttgca ggaatcaatg gaatagcgga gcagctgggg atggtcaccc gggctctttc
     3781 actggagctg gatgaacttg gtgccctcaa aatgccgtgt attctccact gggatttcag
     3841 tcacttcgtc gtgctggtca gcgtaaagcg taaccgttat gtactgcatg atccggccag
     3901 aggcagaaga tatctcggtc gggaggaaat gagccggtat tttacgggca ttgcacttga
     3961 ggtctggccc ggaagtgaat tcctggcgga aacccagcag acccgcataa gtctccgttc
     4021 actgattaac agtatttacg gtattaaaag aacactggcg aaaattttct gtctgtcagt
     4081 tgtaattgaa gcaatcaatc tggtaatgcc ggtgggaact cagctggtta tggatcatgc
     4141 gattccggcg ggggacagag ggctgctgac gcttatttct gctggcctga tgttctttat
     4201 attgctcagg gccgcggtga gtatgctgcg tgcatggtcc tcactggtta tgagcacgct
     4261 catcaatata cagtggcagt cgggtctgtt taaccatctt ctcagactgc cgctggcgtt
     4321 ttttgaacgc cgtaaattag gtgatatcca gtcgcgtttt ggctcccttg acactttgag
     4381 ggccaccttt accacctgtg ttgttggggc aatcatggac agtattatgg ttgtgggtgt
     4441 ttttgtgatg atgctgttat atggaggata tcttacctgg atagtgctcg gttttaccat
     4501 ggtttacgtt cttattcgtc tggtgacata cggctattac cggcaaatat cggaagaaac
     4561 tcttgtcagg ggggcccggg ccagctccta ttttatggaa agcctgtatg gtattgccac
     4621 ggtaaaaatc cagggtatgg ctgggattcg gggaacacac tggcttaacc tgaaaataga
     4681 tgcgatcaat tcaggtatta agttaaccag gatggatttg ctcttcgggg ggataaatac
     4741 ttttgttgcc gcctgtgatc aggtggcgat tttatggctg ggtgcaagcc ttgtgatcga
     4801 taatcagatg acaataggga tgtttgtggc atttggttct tttcgtgggc agttttcgga
     4861 tcgggttgct tcgctgacca gttttcttct tcaactgaga ataatgagtc tgcataatga
     4921 gcgcattgca gatattgcac tacatgaaaa ggaagaaaag aaaccggaaa ttgaaatcgt
     4981 tgctgacatg agcccggttt cactggaaac cactgattta agctaccggt atgacagcca
     5041 gtcagcacag gtattcagtg gtctgaattt gtctgtggct ccgggagaaa gtgtggctat
     5101 aactggtgcc tccggtgccg gaaaaaccac attaatgaaa gtattatgtg gactgtttga
     5161 accagatagt ggaaaagtac tggttaatgg cacggatata cgtcaacttg gaataaataa
     5221 ttatcaccgt atgatagcct gtgttatgca ggacgaccgg ctattttcag gatcaattcg
     5281 tgaaaatatc tgtgggtttg cagaagaaac agacgacgaa tggatgacag aatgtgccag
     5341 agcaagtcat attcatgatg tgataatgaa aatgccaatg gggtatgaaa cgttaatagg
     5401 tgaactgggg gaaggtcttt ccggcggtca aaaacagcgt atattcattg cccgagcttt
     5461 ataccggaaa cctggaatat tatttatgga tgaggctaca agttctcttg atacagaaag
     5521 tgaacgtttc gtgaatgctg ctataaaaaa gatgaatatc acccgggtga ttattgcaca
     5581 tagagagaca actttaagaa cagttgatcg gatttttaga attacaaatt aggataatat
     5641 atcactaaaa gcagcattgc tgcataataa gctatgctgc tttagaaatt aatcaacttc
     5701 cactactaga agaggatgtt gatgagttaa atcctgagtt taataaacct ccattgtagt
     5761 ttggattaaa acttccaagt cctatacccg gattatagtt aataccgttt gatggtattg
     5821 ttattgtcct atttgaacca tcagcaataa ctcctccaat atatcctcca attgcacttc
     5881 ctgctatatc cgcaccaggg atttttgttg ctttacctaa aaaaccacca gccgcagtac
     5941 ctattgcaac ggatgcatct ttatctaatc ctgcaccatg tattaaagca agttcatcgg
     6001 ttgt
//
LOCUS       DAZFRG010000265         5978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFRG010000265 DAZFRG010000000
VERSION     DAZFRG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.267"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..1262
                     /gene="ompC"
                     /locus_tag="ACHKUC_004270"
     CDS             135..1262
                     /gene="ompC"
                     /locus_tag="ACHKUC_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6620413.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVVDNVTHNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1374..2429
                     /gene="apbE"
                     /locus_tag="ACHKUC_004271"
     CDS             1374..2429
                     /gene="apbE"
                     /locus_tag="ACHKUC_004271"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO6620414.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2503..3567
                     /gene="ada"
                     /locus_tag="ACHKUC_004272"
     CDS             2503..3567
                     /gene="ada"
                     /locus_tag="ACHKUC_004272"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO6620415.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGCCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3567..4217
                     /gene="alkB"
                     /locus_tag="ACHKUC_004273"
     CDS             3567..4217
                     /gene="alkB"
                     /locus_tag="ACHKUC_004273"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO6620416.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLAGDCRYNLTFRQAGKKE"
     gene            4293..5936
                     /gene="yojI"
                     /locus_tag="ACHKUC_004274"
     CDS             4293..5936
                     /gene="yojI"
                     /locus_tag="ACHKUC_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO6620417.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1404 a         1505 c         1632 g         1437 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acgacaagtc tgtagatggc gaccagacct
      301 acatgcgtct tggcttcaaa ggtgaaactc aggttactga ccaactgacc ggttacggcc
      361 agtgggaata tcagatccag ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg
      421 tggcattcgc aggtctgaaa ttccaggatg tgggttcttt cgactacggt cgtaactacg
      481 gcgttgttta tgacgtaact tcctggaccg acgtcctgcc agaattcggt ggcgacactt
      541 acggttctga caacttcatg cagcagcgtg gtaacggctt cgcgacctac cgtaacaccg
      601 acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggt aaaaacggta
      661 gcgtagatgg cgaaggcatg accaacaacg gtcgtggtgc tctgcgtcag aacggcgacg
      721 gcgttggcgg ttctatcact tatgattacg aaggcttcgg tatcggtgct gcagtgtcca
      781 gctccaaacg tactgacgct cagaatggta cttacgtagt tgataatgta actcataact
      841 acatcggtac tggcgaccgt gctgaaacct acactggtgg tctgaaatac gacgctaaca
      901 acatctacct ggctgctcag tacacccaga cctacaacgc aactcgcgta ggttccctgg
      961 gttgggcgaa caaagcacag aacttcgaag ctgttgctca gtaccagttc gacttcggtc
     1021 tgcgtccgtc cctggcttac ctgcagtcta aaggtaaaaa cctgggtgtc atcaatggcc
     1081 gtaactacga cgacgaagat atcctgaaat atgttgatgt tggtgcgacc tactacttca
     1141 acaaaaacat gtccacctac gttgactaca aaatcaacct gctggacgac aaccagttca
     1201 ctcgtgacgc tggcatcaac actgataaca tcgtagctct gggcctggtt taccagttct
     1261 aatctcgatt gatatcgaac aaagggcctg cgggcccttt tttcattgtt ttcagcgtac
     1321 aaacacagtt ttttggtgta ctcttgcgac cgttcgcatg aggataatca cgtatggaaa
     1381 taagctttac ccgcgtggca ctgctggctg ccgcgctctt ctttgttggt tgcgatcaaa
     1441 aaccacaacc cgccaaaacc cacgctactg aagttaccgt tcttgaaggc aaaaccatgg
     1501 gtaccttctg gcgtgccagc attccgggca ttgacgccaa acgcagtgcc gaacttaaag
     1561 aaaagattca gacccaactg gacgctgacg atcagctgct ttcgacctat aaaaaagatt
     1621 ccgcgctgat gcgctttaac gactcgcaaa gtttgtcgcc gtggccggta agtgaagcga
     1681 tggccgatat cgtcactacc tcgctgcgca ttggcgcaag gaccgatggt gcgatggata
     1741 ttaccgtcgg gccgctggtg aatctgtggg gctttggtcc ggaacaacaa ccggttcaaa
     1801 ttccgagcca ggaacagatc gatgcgatga aagccaaaac cggcttacag cacctgacgg
     1861 tcattaatca gtcgcatcag caatatctgc aaaaagacct gccggattta tatgtcgatc
     1921 tctccaccgt cggcgaaggt tatgcggcgg atcacctggc acgcttgatg gagcaggaag
     1981 ggatttcccg ctatctggtg tcggtgggcg gcgcgctgaa cagccgtggt atgaacggtg
     2041 ctggccagcc gtggcgggta gcgattcaaa aaccaaccga taaagaaaac gcggttcagg
     2101 ccgtggtgga tatcaacggt catggtatta gcacctctgg cagctaccgt aactattacg
     2161 aactggacgg caaacgtctt tctcatgtta tcgatccgca aaccgggcgt cctatcgaac
     2221 acaatctggt atccgtgacg gtgattgccc cgacggcgct ggaagccgat gcctgggata
     2281 ctggcttgat ggtactgggg ccggagaaag ccaaagaggt tgttcgtcgg gaagggctgg
     2341 cggtttatat gatcaccaaa gagggtgata attttaaaac ctggatgtcg ccgcagttta
     2401 aaagcttcct tatcagcgaa aaaaattaaa gcgcaagatt gttggttttt gtgcgatggt
     2461 gaccaggcag actaaaggct atccttaacc agggagctga ttatgaaata cgccacatgc
     2521 ttaactgacg atcaacgctg gcaatctgtc ttagcccgcg actcgaatgc cgacggcgaa
     2581 ttcgttttcg ccgtgcgcac cacgggtatc ttttgccgtc cgtcttgccg agccagacat
     2641 gccttgcggg aaaatgtctc cttctacgca aatgccagcg aggcgcttgc cgctggcttt
     2701 cgtccctgca aacgttgtca gccagacaaa gccaatcccc ggcaacatcg ccttgataaa
     2761 attacccacg cctgccgact tctggaacag gaaacgcctg taacgctgga agccttagcc
     2821 gaccaggtgg cgatgagtcc atttcatttg catcggttgt ttaaagcgac taccggaatg
     2881 acgcctaaag cctggcaaca ggcctggcgc gctcgccgtt tgcgcgaatt gctggcgaaa
     2941 ggggagagcg tgactacatc tattattaac gccggattcc ccgacagcag cagttactac
     3001 cgcaaagccg acgaaacgct gggcatgacg gctaaacaat tccgtcacgg tggcgaaaat
     3061 ctggcggtgc gttacgcgct ggctgattgt gagctgggct gttgcctggt ggcagaaagt
     3121 gagcggggga tttgcgcgat attactgggc gatgatgacg cgacactaat cagcgagtta
     3181 cagcagatgt ttcccgctgc cgacagcgcg cctgccgatc tgacgtttca gcaacatgtg
     3241 cgtgaagtga tcgccagcct caatcaacgc gatacgccgc tgacgttacc gctggacatt
     3301 cgcggcactg cttttcagca acaagtctgg caggcactgc gcacgatacc ttgcggtgaa
     3361 accgtcagtt atcagcaact ggctaatgcc atcggcaaac cgaaagcggt acgggccgtt
     3421 gccagtgcct gtgccgccaa caagctggct atcgttatac cttgtcatcg ggtggtccgt
     3481 ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc gcaaagcgca actgctgcgc
     3541 cgcgaagctg aaaatgagga aaggtaatgt tggatctgtt tgctgatgct gaaccgtggc
     3601 aagaaccact ggcggctgga gcggtgattt tgcaccgttt tgcttttaac gctgcggagc
     3661 aactgatccg cgatattaat gacgttgcca gccagtcgcc gtttcgccag atggtcactc
     3721 caggcggata taccatgtcg gtggcgatga ccaactgtgg gcatctgggc tggacgaccc
     3781 atcggcaagg ttatctctat tcgcccattg atccgcaaac aaataaaccg tggcccgcca
     3841 tgccgcagag ttttcatcat ttatgtcaac gtgcggctac ggcggcgggc tatccagatt
     3901 tccagccaga tgcctgtttg atcaaccgct acgttcctgg cgcgaaactg tcgctgcatc
     3961 aggataaaga cgaaccggat ctgcgcgcgc caattgtttc tgtttctctg ggcttacccg
     4021 cgatttttca atttggcggc ctgaaacgaa atgatccgct aaaaagattg ttgttggaac
     4081 atggcgatgt ggtggtatgg ggcggtgaat cgcggctgtt ttatcatggc attcaaccgt
     4141 tgaaagcggg gtttcatcca ctcgccggcg actgccgcta caacctgaca ttccgtcagg
     4201 caggtaaaaa agaataaaaa taagaattat tattgctgtg cgcacgaaga tgtttaaact
     4261 gcgggctgta attcattgtc cgggttttct gcatggagct tcttgtactt gtctggcggc
     4321 agtatcgctg gccatttatc agtgtgatgg cgctaagcct cgccagtgcg gcattaggca
     4381 ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac ggcggatacc agtctgctgg
     4441 tattgccgga gtttctgggg ttattgctgc tgttgatggc cgtcactctc ggatcgcaac
     4501 tggcgctcac cactttgggg catcacttcg tttaccgact gcgtagtgaa tttatcaagc
     4561 ggattctcga tactcacgtc gagcgcattg aacaactcgg tagcgcctcg ttgctggcgg
     4621 gattaaccag cgatgtgcgc aatatcacca ttgcttttgt gcgtctgccg gaactggtgc
     4681 aggggatcat tctcactatc ggttcagcgg cgtatttgtg gatgctgtcg ggcaaaatgt
     4741 tgctggtaac ggctatctgg atggcagtca ccatctgggg cggttttgtg ctggtggcgc
     4801 gggtgtacaa acatatggcg accctgcgtg aaaccgaaga taagctgtac acggattttc
     4861 aaaccgtact cgaagggcgc aaagagctga ctctgaaccg tgaacgcgcc gagtatgtgt
     4921 ttaacaacct ctacattcct gatgcgcaag agtatcgcca tcatattatt cgcgcagaca
     4981 ccttccatct tagtgccgtg aactggtcaa acatcatgat gctgggcgct atcggcctgg
     5041 tgttctggat ggcgaacagt ctcggttggg ctgataccaa cgttgccgcg acctattcgt
     5101 tgacgctgtt attcctgcgt acgccgctgc tttcggcggt tggcgcatta ccgacgctgc
     5161 tgacggcgca ggtggcgttt aacaagctga acaaattcgc gctcgcgcct ttcaaagcag
     5221 agtttccgcg cccgcaggct tttcccaact ggcaaacgct ggagctgcgt aacgtgacgt
     5281 ttgcttatca ggataacgcg ttttccgttg ggccgattaa cctcaccatc aaacgtggtg
     5341 aactgctgtt tctgattggt ggcaacggta gtggaaaatc gacgctggcg atgttgctga
     5401 cgggcttgta ccaaccacaa agcggcgaaa tcttgctgga tggcaaacct gtcagcggcg
     5461 aacaaccgga agattatcgc aaactgtttt cggcagtgtt taccgatgtc tggctgtttg
     5521 atcaactgct ggggccggag gggcaacccg ctaacccgca actggttgag aagtggctgg
     5581 cgcagctgaa aatggctcat aagcttgagt taagcaacgg gcgtattgtt aacctgaagt
     5641 tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc gctggcagaa gaacgcgata
     5701 ttatcctgct ggatgaatgg gcggcggatc aggatccaca cttccgtcgt gagttttatc
     5761 aggtgctgct gccgctgatg caggagatgg gtaaaactat tttcgccatc agtcatgatg
     5821 atcattactt tatccacgcc gaccgcctgc tggaaatgcg caacgggcaa cttagcgagc
     5881 tgacaggcga agagcgcgat gctgcttcgc gtgatgccgt tgcccggacg gcataatatt
     5941 acgacgctga cctggcggac tgctgccggc aggtcaat
//
LOCUS       DAZFRG010000266         5924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFRG010000266 DAZFRG010000000
VERSION     DAZFRG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.268"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            179..760
                     /gene="mdaB"
                     /locus_tag="ACHKUC_004275"
     CDS             179..760
                     /gene="mdaB"
                     /locus_tag="ACHKUC_004275"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO6620418.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            791..1105
                     /gene="ygiN"
                     /locus_tag="ACHKUC_004276"
     CDS             791..1105
                     /gene="ygiN"
                     /locus_tag="ACHKUC_004276"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO6620419.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1150..2037)
                     /locus_tag="ACHKUC_004277"
     CDS             complement(1150..2037)
                     /locus_tag="ACHKUC_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6620420.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2034..2981)
                     /locus_tag="ACHKUC_004278"
     CDS             complement(2034..2981)
                     /locus_tag="ACHKUC_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6620421.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(2992..4029)
                     /locus_tag="ACHKUC_004279"
     CDS             complement(2992..4029)
                     /locus_tag="ACHKUC_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6620422.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4038..5021)
                     /locus_tag="ACHKUC_004280"
     CDS             complement(4038..5021)
                     /locus_tag="ACHKUC_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO6620423.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5018..5827)
                     /locus_tag="ACHKUC_004281"
     CDS             complement(5018..5827)
                     /locus_tag="ACHKUC_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6620424.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1674 a         1603 c         1374 g         1273 t
ORIGIN      
        1 ttatgggaag ttgatttaag tattgtcatt ttcagaaaat atttatcccg catcggtgaa
       61 tcagagaaag atcagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttc
      121 accgtaccgc tctgtgacgt actatagtcg gcaaacgtct caccttgagg ttaaaaaaat
      181 gagcaacatc ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa
      241 cgacaccctg accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat
      301 tgttcgcgcc gacagcgact acgatgtcaa agcggaagta caaaactttc tctgggctga
      361 tgtggtgatc tggcagatgc ctggctggtg gatgggcgcg ccgtggacag tgaaaaaata
      421 tattgatgat gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg
      481 caaagatcca tcaaaaaaat acggttccgg cggcctggta cagggcaaaa aatatatgct
      541 ttctctgacc tggaacgcac caatggaagc cttcaccgaa aaagatcagt tcttccacgg
      601 cgttggtgtt gacggtgtgt acctgccgtt ccataaagca aaccaattcc ttggcatgga
      661 accgctgccg acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac
      721 tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa ctagagctct ggctttagaa
      781 ggagttaacc atgattacag taatcgcaga aattcgtact cgtcctggtc aacatcaccg
      841 tcaggcagtg ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg
      901 ccacggctat gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc
      961 ggattctatc gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca
     1021 aaccccgcac atgaaagcat atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat
     1081 tcgtatcctg cagccggggc tttgacattt ctaaaaagag gccggtgctg ctaccggtct
     1141 cagcccagat tagccattct ttcttcccgt tttcagtcct cgatggaaat cgttaattcg
     1201 cttaatggtt ttaaccgcac gctgcggcac tgcggaagct accgaccaga caatcatttc
     1261 cagacacaac aataccgtac catgtagcgg cgttttccct ttttcatccc cgcgcggaac
     1321 atgaatcacg atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat
     1381 aacagggatc cccagccgcc tcgcctcacg aagcgttgtc agcccttccc ggtgcgcaga
     1441 tttttgcgcc atcatgatca gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag
     1501 gccaataccc gtacggttta gtgccgtggc aggcaaacct atgcggttga atagtcgtgc
     1561 ggtatagtcc gccagaatac ccgaagcgcc aatgccaaaa attgcgactt gccgtgcctg
     1621 caccaacaac gctacagctt gcgcaagcgc gtggcggttt tcaggctctg acaggacatt
     1681 acaggtatac aaatgccctt caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa
     1741 gttactcacc gtcgtagaca tcttttcgct ggaactaagg gctggaccaa gccactgttc
     1801 cagagtgcgt ttcagatcac gcagcccggc aaaccccagg gcctgtatag ccctgataac
     1861 tgtggcatcg gaagtgttca acgtggccgc aatttccatc gccgtttgct cgagtaccac
     1921 ttcacgatgc tcattaatgt agctggcgac aactcttaac ccaggcgtta actgactcgc
     1981 gcgagtgcga aaacgctcac caaaaatatc aacccgcact ttatctttct ggatcattta
     2041 gcctccggta gaggtcgccc tgcaatttgt gattgtatct gcgcagccat cagccccaga
     2101 gatgcaaagg agttagaaat tgcctcttca cgagaaatca gcacataacg cccggaacga
     2161 caggcagtca gaaactgaca ccatcccggc atgacttttt ccattgctgc cagctcatcc
     2221 tgaggtttac caccggtgtc accgcgccag gttgcaaaaa caaaatcggc atccagttca
     2281 ggtaaacgct ccgcgctcac atccattcgc ccaccttcag gaatgctctc aatcagcggc
     2341 ggaaaacgaa aaccagcatc gcgtaatacc cgtcccagcg aatgatagct atgcatcaca
     2401 ttaatttttc cctggtttgc ctgaatcacc gataccgtta ttttttggct gtccagcgtc
     2461 gccttcagcg cattgatttg tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc
     2521 gttcctgtaa gttctgccag cttgcgatag atttctggcg cgcccccttt gagatgatca
     2581 atgctcaccg tgggcgcaat tttttcaagt tgttcaatgg gcgtattacg ggtcggttct
     2641 gtgatgatca agtcaggctt tgccgccaca atcgcttcaa tatcaatatc agcggtacca
     2701 ataaaggcga tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc agatcgaatg
     2761 aaatggctac cgtcaggccg agttcgcccg tggctggcga caggaggtac accgagctca
     2821 atcagaggaa tagtgatatc aagatcgtgt aaagagacaa ttctctttgg atgagcagga
     2881 acagtgactt tgcgccctaa atcatcagta aatacctgaa caggttctgt tgcacgggaa
     2941 aaaaatgaca acagtatcag cagagaaaaa aataaacgca tataactccc gttaaaaact
     3001 gttcctgcgc tgccagagca gtagtaaaaa aaatggtcca ccaatcagtg agataatgat
     3061 tccggcaggc agttgcaaag gcaaaaaagc caaacgacca atgctatccg ccagaataac
     3121 taacaacgcc cccgataccg cgctccccag cagtaatgtc gtctgcccgc cacgtagaat
     3181 aaaacgtgat atatggggtg ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac
     3241 gcagacggct gtcaggatta tgggtgcggc gacacgtaac aacgccagac gagaactatt
     3301 cacccctaac ccggttgcaa cctgatgacc aagcagagcg atatccgcag tcctggcggt
     3361 gaacagcaat aaaagagcgg ctggcaacat ccaacaggct gaaataccga ctaacatcca
     3421 gttagctgca tgcaggcttc ccgataacca catcaatgca gtttgtacat ctcgaacatc
     3481 tgcagtggtc ataaatacac cgataccggc ggcgaaaaac caggaaacac caataccaat
     3541 cagaacaaat cgcgggcggg aaatatcgcg cgcacaaaaa atcacgatta aagccaccaa
     3601 caggccacca gcaagaccag ccactggacg ccagaacatt cccagcattg gaaactggaa
     3661 aatcagccac agcacagcca cgctacatcc ttctttgacg ccgattaaac caggatcggc
     3721 aaggccgtta cgtgcaatgg attgcattgc ggcccctgcc atacctaaca tggccccgca
     3781 tagcaatgcc atgatgactc tcggcaagcg aatatcctgc acaatataac gtgcgtctgt
     3841 gcttacgttt tctggagcaa acaacgctcg tcctacttcc gatgccggaa cagagaaaga
     3901 accctgcatc agaccgaata tcagtattcc aaccgcaagc aggaacagcc caccaagcaa
     3961 cagcagattt ttagggcgca ccagtgtcga aaacttacca acacgtagtg ggcgtagccc
     4021 gacgttcatt atccgactca tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca
     4081 ccaactaacg ccgtcatgac accggtcgct aactcatggg gcgtgaaaag cgtccgggca
     4141 ataatatctg ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg
     4201 cgcagatccg ctgataccag acgccggaca atctgcggta ctagcaggcc gacaaaacca
     4261 atggggccag caatcgatac ggctgcgcca cagagcaacg cgatcgcaag cagggtgaat
     4321 gttcgcgtgc ggagtacaga gacgcccagc ccctgcgcca tgcgatcgcc cagtgccaac
     4381 atgttaaggg agggtgcaag gtaaagggct aacacaaatc cccccagaga aaaccaggcg
     4441 gacgtaccca gcgtcgccca atcctgcccc gctaaatcac ctgctagcca tgtccgcatc
     4501 gccagcagtg tttgttcgtc aagaatcagg attgcagccg ttattgagga cacgaacgcc
     4561 gatagcgcca cgccgcataa agtaactttc attggcgtta accctgaacg ccctgcggag
     4621 gaaagcagca atatcaacag gaacaataac gcgccccccg tagatgccag cagtgggcgt
     4681 ccaaccggaa aggccagtcc aagcgcactg gcggcaacga cagcaagagc ggctccggca
     4741 tttaagccta aaatatgtgg ttcccccaat gggttacgaa tgacagcctg caacaaagca
     4801 ccagcaacac caagagaagc accggtaagc aatgctgccg ctaaacgcgg caaacggagt
     4861 cttacaatta cgttgtgatc gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc
     4921 gtttgtggtg caataaatcg ggcaccaatc cctggatata ccacgcatcc caccagcagt
     4981 aaggcgagga aaattacagt aaccagccct gtgcgcatca tactttttct acaccacgaa
     5041 acggcatgaa gaacggcttt cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa
     5101 agacagcttt taccagttca ggcgtgcaat gctccccctc attcaacaca cgcaccactt
     5161 tcccctggcg taaaaatatc aacgtatcgc cataattgac ggcaaaattc agatcatgca
     5221 acaccacgac gactgtacgc ccgtgatgac gcgttaaatc atgtaataac tcaagaattt
     5281 caacctgata acgtaaatca agccaggttg tgggttcatc cagcaggata taaggcgtct
     5341 tttgcgccaa caccatcgca atccagcaac gctggcgctg cccaccagag agtttttcaa
     5401 caggtaaatg tgcaaactcc tgcgtaccgg ttaatttcag ggcctcttcc accgcctgct
     5461 catcggcgtc gctccattga cgtatgaaat tttgccaggg aaaacgccca cgcgacacca
     5521 gttcataaac ggttaagccc tccggcaata atggcgactg cggcaagatc ccaagtctac
     5581 gggataacgc tttcgttggt tgctcgtgaa tcgctttgcc atcaagcaat atcgaaccgc
     5641 ccataggttg caagatacgt gcaatggtgc tgagcaacgt cgactttcca cagccgtttg
     5701 ccccaaccag aacggtcatt ttttctgtgg gaacggagaa agaaacacca tcaacaatga
     5761 tcttcttgtg atacccggca gaaacattgt cgaggatcag cccctgccct tttgcgttat
     5821 gtgccacgtg tacgccaact taatcaaaga caaacaaaaa taaaactcat tctcctttga
     5881 gagtttgacg ccacaaaatg tagtagtcaa gcagttaaaa gcca
//
LOCUS       DAZFRG010000267         5898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFRG010000267 DAZFRG010000000
VERSION     DAZFRG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.269"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..128)
                     /locus_tag="ACHKUC_004282"
     CDS             complement(<1..128)
                     /locus_tag="ACHKUC_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6620425.1"
                     /translation="MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEP"
     gene            619..828
                     /locus_tag="ACHKUC_004283"
     CDS             619..828
                     /locus_tag="ACHKUC_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620426.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(894..1427)
                     /locus_tag="ACHKUC_004284"
     CDS             complement(894..1427)
                     /locus_tag="ACHKUC_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620427.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(1430..2356)
                     /locus_tag="ACHKUC_004285"
     CDS             complement(1430..2356)
                     /locus_tag="ACHKUC_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFO6620428.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            2533..2931
                     /locus_tag="ACHKUC_004286"
     CDS             2533..2931
                     /locus_tag="ACHKUC_004286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620429.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3135..4049)
                     /locus_tag="ACHKUC_004287"
     CDS             complement(3135..4049)
                     /locus_tag="ACHKUC_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6620430.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
     gene            complement(5356..>5898)
                     /locus_tag="ACHKUC_004288"
     CDS             complement(5356..>5898)
                     /locus_tag="ACHKUC_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFO6620431.1"
                     /translation="VKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTA
                     VRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSP
                     REYLRQRACNGLSDNRCLEI"
BASE COUNT         1594 a         1563 c         1270 g         1471 t
ORIGIN      
        1 ggttcaaagc tctgctgaac aattgcgatc ttttcctgtg tggtacgccg tctgcgtttc
       61 tccggcccta agacatcaat catctgttct ccaatgacta gtctaaaaac tagtattaag
      121 actatcactt atttaagtga tactggttgt ctggagattc agggggccag tctaaccata
      181 tgtcagggat aatgtgcaaa tggaggtatc ggaactgcat ttcagttcat tattaattaa
      241 tgacaaaaaa aacctctgcg agcgcagagg gggtaaggaa tgttcccggt cataatatgt
      301 cgatagtgtt tatatacata cagaacaaga acaacaacca gccaactaat aaatatttta
      361 ttttttagag ctgaaacctt aaagctcact tccattccat acattgttat aaaccttatg
      421 ttgaaaactg aaagtaagta cggcagactt attaagttgc gtgcagtctt ttcacgttat
      481 acaaacaaga acacctgcga agttatttct ccttacatat ccccctatgc caacggggga
      541 ttttttctgg ttcactaacg cataaataat agtgacaccc tatcattcac aaaccgtgat
      601 aatataagga ttatcatcat ggcaaaatta ggtgaaaaca ccccacatat tatcgatgct
      661 actgtagatt ttatggcatc aagccaggca tttcgtgagt atctgaaaaa acttccccct
      721 gataacacaa taccgccagc tcttactgat gagcacgtac cgttatatat acgaaggctg
      781 gaatactatc gtctgcttta tcgaccaaag cagacagaat catattgaaa aaaacggtga
      841 acggttcgcg aaatcctttc gcgggtcgtg aggcgttgga caagcgaagc gcgtcagttt
      901 ttccggctct tccccgtgac aggtttctcc accagtttat cttccagtgt cacaactttc
      961 tgatacaccg ccgctgtggc gttgtagttc ctgtcgagta ttgttttcat tccccatccg
     1021 agcacacaga acataaccag aaaagccact gacaccccac ccagccattt ccagctatac
     1081 cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat atccctgttc
     1141 agggcatccg cgtttgcctt catgtttgcg actgactctt ccagccgctg aaaatcctgg
     1201 ctgatgcgct cacctatcag tcctgccacg tccagcttct gcacctcatt tctgacttca
     1261 tccctgacct gttttgccac accgtcagaa atatcccgat atccgcgaag agtttccctt
     1321 gccacagcca gagaatcagc aacagctttc tgttgctcca ttgcggtatg ttgcagatgc
     1381 tccaccgata acaggagcgt gagtaatttt tcttctgtgc tggccatgtt cagaatccca
     1441 tagaaaatcc acgtgacggt gatttaaccc gttcaggtgc agttcggttc tgtctgctca
     1501 ctctgtctct catgtcaaag ccatgctccc gcgcaacatc atgttgcagc cttctgacca
     1561 gttcatgttg cttaaccgga gtgtccagcc attccctgat gtcatgcccg accgttcgcg
     1621 cctgttcaag atggcgggaa tagtccggtt cagaccgttt ttgcatgatg tcatgacgct
     1681 gggcatcatc aagccgtccg gcagcttcaa aacccagatg cacttcggga cgtcgttcaa
     1741 tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct ttcaaagcca tgctttctca
     1801 tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc tctttttgct
     1861 gagtgagagt caggcttccg gaatcctttt tcgcacctcc ccgctcggga tacctggcgt
     1921 tatagcgccg gaaatactgt tcaggtgtac gttcaatgcc atcattcaca cgctgactca
     1981 tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct cgctttcgga ttgtgaatgg
     2041 caaaactcca ggcgtgccgt tcgccagctt cctgacgtac aaaatcctgc accagttcaa
     2101 ggcgctgttc cggtgtcagt tcacggggta atgcaatttc aagttcccta taggtcgaac
     2161 cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg ggaaggatta tccctggccc
     2221 attccggcat gttcccgtgt gcggaatatt ccaaatcctg acggtcgcgg tatttctctt
     2281 tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt gccaccaaat ttaacgctga
     2341 ggtgataggt tgccacggtt tcagccctct gcaggagcgc actgtttctc gacgaaggag
     2401 agaaacatat aagtgcgctt ccttctaatt ttacgaatta gcacggaaga taccccctgc
     2461 acccgccgac cgcaagcgat atactgcacg accacaacac accgcaaaaa aacaccaggg
     2521 agcatcacga acatgcgcat cagcaacatc gaatggctga agaaacggat cggattcatc
     2581 aggaagctcg gagaacagac cgcacgacag agacagatta ttgacctgat cgacaacgaa
     2641 gccggactca cagaacagga acgcaaactt ctccacgtac tggcaacagc ggaaaaaaac
     2701 gacttacagg cacaggagag cgaacgaaaa caggcggtcc aaaaacgaat cgagggaaaa
     2761 aaacaacgac gggaacgcaa tcaccggctc tttctcgcgg ctgggctact gatcgaagca
     2821 ggactggtag acacaaaaac aggcgagcta tgctataaaa aagacaggat attacaagca
     2881 cttaaggaac ttaagtacga tctggagaca tccccaaacc cagacgcgta agtgcctcca
     2941 tccgtgttcc gattccctgg tgcttcctcg ctcattcgct cgctacgctc ggtcatgaga
     3001 ctgcggcgag cactaccacc acaatcacgc ggaaagccgc gccattgcag ggctgaacgg
     3061 ggaaaaacgg caaaaaacct gctgctcaaa aagacgccag aaaggcgctg ttatctgata
     3121 taggcttatt tggtctactc tacgagacct cgttttttca gttggtaata ccaggcgcga
     3181 ctgatcccta atgcctccca cggtttcagc gtgcgtgcag atgtagctac tgtgctgcgc
     3241 ttagacttag aaccgccctt gcgaccgcgt gcagcctgaa tttctggcgt gtgcgtatca
     3301 gccacatact gtgcgaaagt ttccggcgtg aagttcctgt gcgtgtattt cgcaatgctc
     3361 ttgccaatag cccgacattc agccagtgaa agcggaacgg gaagcgatgc attgtacatt
     3421 tcgacacgct ggatcaccgc ttcaagccac tgtgagaacg ctggccagcc ctgacgaatc
     3481 gccctgtaag cccatttgcg cgtcatttcg aacaggtggc agtttcgccc catcccgtaa
     3541 tgtttatcga tgctacgacg ctctgaggcg ctcaaatcga gataatcagc cagttcatcg
     3601 agggtatagg cttcctcgcg ccactccatc accagccatt ccaggtggaa cggatttttg
     3661 caaatcaggc cgctgtagtt cacatccgca caaagtttct cacacagcga acgttcaacc
     3721 gctgcggcat atttcagcgc cttaaccgag gcatcaggcg cggttctcac ggcgatgttg
     3781 agtgcgtaga gcaggtgagc gtgtccgttc acaggatttt taacggtgat gttcggcgcg
     3841 ggagcgttcc ggtcgctcca gtcaatcgca gccccctcgc ggtcaacgtc aaaaaccagc
     3901 cagaactgtg catgaggctg gttctgctgg atataccgtg caagaagcgc gcgccctttg
     3961 ccggagatac gaaggccgaa agcgagatca tccgtgtgat agggacgatg gggtaaattt
     4021 tcgtcgaagt attgaagcgc ggcgctcatg tgttacgatt ccttccaggt tatcgaccga
     4081 catcgcccct gtattttcgt gaaagtcttg gcggatagac acgaatttca ggggcgtttg
     4141 tttttcagtc gcttacacta cccccaacga caaaacctgt cgagccttcc cgcaaatccg
     4201 atccatttac cataactccc gttgtacgta ccccttaaaa cgccctcagt cgctttctgg
     4261 cgcaaaaaac gcaaaaagac aaggaaacac caggccaaac cagaaaaacg cgtcagaatc
     4321 gttttcaggg cgttgcataa ctatgcacga aagcgatgtg cgatcggttt cgtgcgttct
     4381 gaacaccgaa cggctcaaaa cgacaagttt tctcccctgg tgcgtggcgc tctgacgggg
     4441 tttcctgttt ccaaacttca catctcgcca ggacaaaaaa aaaccgacct ggggtcggtg
     4501 gcaagaatca gagtctatgg gaaatatttt agttatcgga tgctcaatat tcacaaacgc
     4561 aaaaaatgcc tcagtgatgc cataaaacaa cctttccaga aggagtccgc gctttacgat
     4621 gcacggaaga gataaaaaac cccacaaaag tggggaacca agagaaacaa caattacagg
     4681 ttgttggttg tgttcgaaca acgatgaagt agctacgcta aataataatt accgtacagg
     4741 ccctacatat tcaacactaa atttaagatc ctgacgaagg atttataaat gcacgaaaac
     4801 acatagacag acaaattcac taaattcata cacaaaaaca agtgtagttt tctaagtaaa
     4861 ttagattcga caaccaatta gtttatccac atcgcattcc actcttgcta caccaatcac
     4921 atttcttgat atgtaatagc agaaaaaata aaaccagaca ccaatagacc aaaaaaacaa
     4981 acattcaaca agaaacattt tgaacaatat cacaaaactg tcacaaccat catcagacta
     5041 ttgaaaaaaa ctggttgctg ttttataaaa gcgactgtaa caacctccac tctatagttc
     5101 cattagttta aagtgtcaca caaactgagc atccatttgt tttccttact tttgcccctg
     5161 tattccaagg ggttttttta taacttaaag attagcacac cacacaaaaa caataaaaaa
     5221 aacgacaagt tatacaataa aaccagtact atccatacag ctaattacat ataaatacta
     5281 ttaaaccatc tggacattaa aaataaactc ttataacata aaattaatgc ggttccggta
     5341 ccattggatt tgcccctata tttccagaca tctgttatca cttaacccat tacaagcccg
     5401 ctgccgcaga tattcccgtg gcgagcgata acccagcgca ctatgcggat gccattcgtt
     5461 ataatgctcg aacgcctctg caaggttctt tgctgccgtt aacccgtctg gtttgggcat
     5521 gatactgatg tagtcacgct ttatcgtttt cacgaagctc tctgctattc cgttactctc
     5581 cggactccgc accgccgtgt tcttcggttc aagtcccaac atccgggcga actggcgtgt
     5641 ttcattagcc cggtagcatg aaccattatc cgtcagccac tccactggag acgacggaag
     5701 atcgttgccg aagcggcgtt ccaccgctcc cagcatgacg tcctgtactg tttcactgtt
     5761 gaagccgccg gtagtgaccg cccagtgcag tgcctcacga tcacagcagt ccagcgcgaa
     5821 cgtgacacgc agtctctctc cgttatcaca gcagaactcg aacccgtcag agcaccatcg
     5881 ctgattgctt tctttcac
//
LOCUS       DAZFRG010000268         5893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFRG010000268 DAZFRG010000000
VERSION     DAZFRG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.270"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1418
                     /locus_tag="ACHKUC_004289"
     CDS             <1..1418
                     /locus_tag="ACHKUC_004289"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6620432.1"
                     /translation="LWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVPREVTEGVI
                     DGLNYPNHEAIDFYHRYKTDIQLFAGMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFY
                     DDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIHFASTVFTRYKEKV
                     KYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDLNEREQIMYQAVHYELVASALAVQ
                     TGKLINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLN
                     YFARKGFNLDITPDDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNP
                     YIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDY
                     FSSHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEIKKRYGMIYVDKDNEGKGTLE
                     RIRKASFYWYRDLIANNGENI"
     gene            complement(1475..1693)
                     /gene="marB"
                     /locus_tag="ACHKUC_004290"
     CDS             complement(1475..1693)
                     /gene="marB"
                     /locus_tag="ACHKUC_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO6620433.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1725..2108)
                     /gene="marA"
                     /locus_tag="ACHKUC_004291"
     CDS             complement(1725..2108)
                     /gene="marA"
                     /locus_tag="ACHKUC_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO6620434.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            complement(2129..2563)
                     /gene="marR"
                     /locus_tag="ACHKUC_004292"
     CDS             complement(2129..2563)
                     /gene="marR"
                     /locus_tag="ACHKUC_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO6620435.1"
                     /translation="MKNTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2775..3440
                     /gene="marC"
                     /locus_tag="ACHKUC_004293"
     CDS             2775..3440
                     /gene="marC"
                     /locus_tag="ACHKUC_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO6620436.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3465..4655)
                     /gene="ydeA"
                     /locus_tag="ACHKUC_004294"
     CDS             complement(3465..4655)
                     /gene="ydeA"
                     /locus_tag="ACHKUC_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO6620437.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4797..4916)
                     /gene="yneK"
                     /locus_tag="ACHKUC_004295"
     CDS             complement(4797..4916)
                     /gene="yneK"
                     /locus_tag="ACHKUC_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO6620438.1"
                     /translation="MVTPVSISNSISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            complement(4994..5875)
                     /gene="yneJ"
                     /locus_tag="ACHKUC_004296"
     CDS             complement(4994..5875)
                     /gene="yneJ"
                     /locus_tag="ACHKUC_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6620439.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
BASE COUNT         1572 a         1385 c         1440 g         1496 t
ORIGIN      
        1 ttttatgggg cggtgcggta gccgcacatc aattggaagg tggctggaat gaaggaggaa
       61 aaggcatcag tatcgctgat gtaatgactg ctggcgctca cggggtgccg cgtgaagtga
      121 cagaaggcgt tatcgacgga cttaattatc ccaatcatga agcaattgat ttttatcatc
      181 gctataaaac agatattcag ttatttgccg ggatgggatt caaatgcttt cgaacttcca
      241 ttgcctggac acgaatcttt ccgcaaggtg acgaacagga gccgaatgaa gagggtttac
      301 aattttatga tgatctcttc gatgaatgcc tgaagcaggg aatggaacct gtggtgacgc
      361 tttcgcattt tgagatgcct tatcatctgg tgacaaaata tggtggctgg cgaaaccgta
      421 aactgatcga cttttttatc cacttcgcat caacggtctt cacgcgctat aaagaaaaag
      481 taaagtactg gatgacgttt aacgaaatca ataatcaggt gaatttcagc gaaagcctgt
      541 gtccatttac taattccggt atcttgtatt cgccagagga agatctcaat gagcgcgaac
      601 aaataatgta ccaggcggta cattatgagt tagttgccag tgccctggcg gtacagactg
      661 gaaaactgat caatcctgaa tttaatatcg gctgtatgat tgctatgtgc cccatctatc
      721 ctctgacgtg tgcacccaac gatatgatga tggccacgaa agcgatgcat cgtcgttact
      781 ggtttactga tgttcatgct cgtggatatt atccgcaaca tatgctgaat tactttgcaa
      841 ggaaaggatt caacctcgat atcacaccag atgataacgc gattcttgcc agaggttgtg
      901 tcgactttat cggctttagc tactacatgt cttttacgac gcaattttcg ccagataatc
      961 cgcaactgga ttatgttgaa ccacgagatt tggtcagcaa cccttatatc gatacatccg
     1021 aatggggatg gcaaattgat ccggcagggc tacgttattc actcaactgg ttctgggatc
     1081 atttccagtt gccgctgttt attgtcgaaa atggatttgg tgcggttgac cagagacaag
     1141 ctgacggcac ggtgaacgat cactatcgca ttgattactt ttcctcccat attcgggaaa
     1201 tgaaaaaagc cgtagttgaa gatggtgttg acttaattgg ctacaccccg tggggttgca
     1261 ttgatctggt ttctgccgga acaggagaaa taaaaaaacg ctacggaatg atttatgtcg
     1321 ataaagacaa cgaagggaag ggaacgctgg aacggatacg taaagcatcg ttttactggt
     1381 atcgggatct catcgccaac aatggcgaaa atatatgaga cttcaggaag atattacgga
     1441 aatgtcaggc tctgtgatca gagcctgaca ataactacat agcgtgttga ttgtaatagg
     1501 gcacgccgag cgcatccgac ttatcactgc cagtacccat gtgatttaaa tcaaacggtg
     1561 gttgttcctg cgatggggga acaaccacga cattgccaca ggaagtaaca accggctgcg
     1621 tggtttgttc cgcaacgccc tgcgcagaaa agagaataag cgcagttgct attgcggata
     1681 aaagtggttt catgattgcc tcagtgacgt tgtcacgtta tcaactagtt gttgtaatga
     1741 tttaatggat gtaaaaaacg cgattcacct tgcatattgg tcatccggta tttatgcggc
     1801 ggaacatcaa agtaattttt gaaggttcgg gtcagagttt gttgggactc gaagccatat
     1861 cgttctgcca gatagagtat cggctcgtta ctttccttca gcttttgcgc gatttccgtc
     1921 atcttacggc tacggatgta ttggcctaat gaatgaccgg tttctttttt aaacatccgt
     1981 tgcaggtgcc atttggagta acccgaacgc tctgacactt tctccagtga cagtggcgat
     2041 tccaggttgt cctcgatcca gtccaaaatg ctatgaatgg taatagcgtc agtattgcgt
     2101 ctggacatcg tcatacctct tttttcgttt acggcaggac tttcttaagc aaatgctcaa
     2161 gtgttgccac ttcgtccgcc gtcaggtttt ttgttaattc ttgatgcagg tcctggccaa
     2221 ctaattgatg gcattgttca catattgccg cgccgctggt ggtaagtttt accagtacgc
     2281 cgcgcttatc attcgggttc ggcaaccttt ccacccagcc tttacagacc aggcgatcca
     2341 gcatacgggt cagtgccccc aggtcgaccg acaacacttt tttcagttca accggagtaa
     2401 tacacgccgc gcagcggata gagcagagca ccttaaactg tgccgcggta atatccagcg
     2461 gagacagata ctcgttaagc aggcgatctt tcttctgatt aaccatatgg attaagcgac
     2521 ccaatggaat aatttcattg aacagatcgc tggtattttt cacattagtt gccctggcaa
     2581 gtaattagtt gcaggggata atattgccca ggcaagtata agtcaaatga atgaattgac
     2641 cgatgccacg ttttgctaaa tcggttcaag ttttggtcaa tgcgatgcaa ggttagctaa
     2701 agagtgacac tttatccttg tttacccctt atacttttcg ctgataaacc agatacacag
     2761 gataacaacc accaatgtta gatttgttta aagcaatagg cctggggctg gtggtgttac
     2821 tgccgttagc taacccatta acaactgtcg cgttatttct tggcctggca ggcaacatga
     2881 acagtgccga acgtaatcgt cagtcgttga tggcctcggt gtacgtattt gccatcatga
     2941 tggtggcgta ttacgccggg caactggtga tggatacatt tggcatttcg attcccggtc
     3001 tgcgaattgc aggcggctta atcgtcgcgt ttatcggttt tcggatgctc tttccgcaac
     3061 agaaagcgat tgattcaccg gaagcgaaaa gcaagtcgga agagctggaa gatgaaccca
     3121 gtgccaatat tgcttttgta ccactggcaa tgccaagtac cgccggtccg ggaaccattg
     3181 cgatgattat tagctcggcc tccacagtgc gtcagagttc aaccttcgct gattgggtac
     3241 tgatggttgc gccgccgctg atctttttcc tggtggcggt tattttgtgg ggaagtctac
     3301 gcagctctgg cgcaattatg cggctggtgg gcaagggggg tattgaagcg atctcccgtc
     3361 tgatgggatt cctgctggta tgtatgggcg tgcagtttat tattaacggc atcctggaaa
     3421 tcattaaaac gtatcattaa aaaaggcccg aatgggcctt tcaactattg cgtctgttct
     3481 tcgagtgtca ctggccagcg gcgaaaaatg atgattgacc atattaacgc agcaaaagca
     3541 ggcaccgcgc ccacataacc aatcatcgac attgaccagt gcagactcac ctgattacct
     3601 accaacgcac ccgcgccgat tccaatatta aatatcccgg agaatagcgc catcgcgacg
     3661 tcggtggcat ctggtgccag cgccagcact ttaacctgca taccaagccc gatgatcatc
     3721 atcgcgatcc cccagaaaat actcagcacc ccgaggtgta tttcactgtt cgccgcaggt
     3781 agcagcagcg ccaggcacac cagcaatagc gcaatcgctg tactcaccaa cgcagacgca
     3841 tactgattac ccagtttacc gaaaatcacg ctgccaataa tgcccgcacc accgagtaat
     3901 aacagtaatg ccgtggcaaa gttggcgctg aatcccgcaa tgttttgtac aaaaggctcg
     3961 atatagctgt atgccgtgta atgggcggtg acaaccacca cagttaacaa ataaatgctc
     4021 atcaatgccg ggcggcggaa caatagcggg aggcttttca atgaaccgga atgctcactg
     4081 ggcagcaagg gaagtaactt aatcaggcac aaaagggtaa taagcgcccc gataccaatc
     4141 gcgaagaagg tcattcgcca gccaaaatac tgccccacaa tgcgcccgag aggtaaacct
     4201 aataccattg ccagtgctgt accggtggca attaaactca atgcctgtgc tcgcttcccg
     4261 gccggagcca tacggatcgc cagagacgcc gtaatcgacc agaaaatcgc atgtgcaaaa
     4321 gccacaccaa tgcgactgat caccagaacg gtaaaactcc acgataaaaa cgacagtacg
     4381 tggctggcaa taaacaccac aaacaggcag atcagtaatt tgcggcgttc aacctggctg
     4441 gtcattaaca taaaaggcaa tgacattagc gccactaccc atgcataaat tgtcaacatg
     4501 atgccgacct gagcggtttg catgtgaaaa ctatgcgcaa tgtcagagag caggccaaca
     4561 gggacaaatt cggtggtatt gaagataaag gcggcgactg ccagcgtaac gacccgtagc
     4621 cacgctactt tgcgggaaac agtgtttgtt gtcataacag attatcgggt gagttgcgaa
     4681 aaagtgatga aatgatctta aaacggttaa tgatgtgaat acaatgcatt tgtgatctgg
     4741 atcgcatttc catctctctg acggttgttc tgtcacgcat cagagagttg ttaacatcac
     4801 attttaatga cgatcaggaa gggggcctgt tcttctttta cttgaggtgc aatattacga
     4861 acgggaaaat catcaggaag agatatggaa ttgctgatgc ttactggcgt aaccatattt
     4921 gccccatata gtcataagtc atcggaacat cactatatag agcatattag ccgtatgaac
     4981 tatcaaaaat agctcattgg taaccctgtt ttgccgagtc aggcgcatcc agcagttgaa
     5041 taaatgcctc aagcggacgc gttttcgcac cacgacgcca gaccagccag gttgttaacc
     5101 aacgccattg ctcagctaac ggccacgctt caacctggtg atgccccggc atactttcca
     5161 gcattgagcg tggaataagc gcaatgcctg ctcctgcgac cacacaggcc aacatcccgt
     5221 gataagactc catctcgtga atagttcccg gagcggcacc gtcagcatga aaccagcttt
     5281 cgaagtggcg acgatacgaa caattggcgc ggaaggcata aatgttactg ccattcacct
     5341 gactggcacg ggttactggc gcatgtcctt gtggcgtgac gatcattagt tcctcgcggt
     5401 ataccggtat cccgtcgatg gcagtatggt taatgggtcc atcaataaac gccgcattca
     5461 gttttccctc cagcacacct tccagcatag tgccggaagg gccggtggaa agtgaaaact
     5521 gaattttggg ataacgacgg ttaaattccg ccagcgtggc cggaatgcgc actgcagcgg
     5581 tgctttccag cgatcccaga gaaaataaac cttgcggctc atcgccagcg acaacgctcc
     5641 gcgcttcatc cactaacgtg agaatttgtt ggctatagcg taaaaagtta tgcccggcag
     5701 gcgagagacg taaacgctga ttctcgcgaa taaacagatc aacccccagt tctgtttcca
     5761 gctggcgtag acgggtagtg agattcgacg gcacgcgatg cacttttgct gcagcctggg
     5821 taatgctgcc agcttcggca accgcgttga acatctccag ttgggtcaga tccatagcat
     5881 tctcgattcg tga
//
LOCUS       DAZFRG010000269         5870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFRG010000269 DAZFRG010000000
VERSION     DAZFRG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.271"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..836
                     /gene="gadW"
                     /locus_tag="ACHKUC_004297"
     CDS             108..836
                     /gene="gadW"
                     /locus_tag="ACHKUC_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO6620440.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1199..4312)
                     /gene="mdtF"
                     /locus_tag="ACHKUC_004298"
     CDS             complement(1199..4312)
                     /gene="mdtF"
                     /locus_tag="ACHKUC_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO6620441.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     TLFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAETMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4337..5446)
                     /gene="mdtE"
                     /locus_tag="ACHKUC_004299"
     CDS             complement(4337..5446)
                     /gene="mdtE"
                     /locus_tag="ACHKUC_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO6620442.1"
                     /translation="MLTACDDKSAENAAAMMPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5554..>5870
                     /locus_tag="ACHKUC_004300"
     CDS             5554..>5870
                     /locus_tag="ACHKUC_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620443.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASM
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVA"
BASE COUNT         1438 a         1480 c         1485 g         1467 t
ORIGIN      
        1 ttggtcctac caaatatggc agtttttgca cacagaaaca gtctggcgtc atttcattag
       61 tatactgaca ttgaaataat cgcagtaatg aaatataagg gatagtcatg gctcatgtct
      121 gctcggtgat cctcgttcgt cgttcattcg atattcatca tgaacagcaa aaaatatcgt
      181 tgcataacga gagtatcctg ctgctggata aaaatttggc agacgatttt gcgttttgtt
      241 cactggatac gcgacggctg gatatcgaag agctgacagt ttgccattac ttacaaaata
      301 ttcgtcagtt gccacgcaat ttaggattgc atagcaaaga ccgtttgtta attaaccagt
      361 caccccccat acagctggtg acggcgattt ttgatagttt caatgacccc cgggtcaatt
      421 cgccgatact gagcaaaatg ctctaccttt cctgtttatc aatgttttct cataagaaag
      481 aactgatccc cttacttttc aatagtatca gtactgtttc aggaaaagtt gaacgcctta
      541 ttagctttga tatcgctaaa cgttggtatc tacgcgatat cgcagaaaga atgtatacca
      601 gcgagagtct catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt aaaatattac
      661 tcgcctccag gatgtcgatg gccagacgat tactcgagtt acgtcaaata cctctgcata
      721 ctattgcgga aaaatgtggc tatagcagta cgtcatactt tataaataca tttagacaat
      781 attatggtgt aacgccacat cagttttcgc aacattcgcc gggtaccttt tcctgacata
      841 tttcgcattt gaatattggt caggatctca cgtctgcttc atgtgaaact ctttcctgat
      901 gatttctgcc gggctaccgg ctagttctct ttcgcagttt gccaggcggt ataggtattt
      961 tcaggaggaa aagactcggc atgtttggga ttattaagct aacaattcat acgattacgg
     1021 gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa tggctggagt ggtttctact
     1081 ttcagtgctg tgtactaagc ctggtattcc tgacggttag ctggttgttg agtggtgaat
     1141 ggttagcagg aaagagtaag gctgaaccct cacgttcaac cttactctca tttacacgtt
     1201 acgctttttt aaagcgggca aagagatgtt ccactacaac gaaaaagacc ggaacgaagt
     1261 aaattgccag cactgttgcg gcaaacatcc cgcccattac gccggtacct accgcgtttt
     1321 gcgcgccaga gccggcacca tgactgataa ccagcggcag cacgccgaga ataaaggcca
     1381 gagaagtcat caggattggg cgtaaacgca tacgcgctgc ttcgatgatt gcctctaccg
     1441 gcgttttccc ttctttctgc atcatctcaa cggcaaattc gacaatcagg atggcgttct
     1501 tggcggaaag cccgatggtg gtcagcaaac caacctggaa gtaaacgtca ttacttaagc
     1561 cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg
     1621 agaacggaat tgaccagctt tcataaagtg cggcgagggc gaggaacacc acgaccagtg
     1681 aaatggcata tagcgcggga gcctggtttg aggataacgc ttcctgatac gacagcccag
     1741 tccatgagta gccgacgcct gccggaagtt ttgcgaccag gtctgccata aatttcatgg
     1801 cgtcaccggt acttttcccg gcagccgctt cacctaaaat ctccattgac gggatgccgt
     1861 tgtagcgttc cagtcgcggt gaaccatagg tccattcggt agacgaatag gcagaaagcg
     1921 gtgccatcgt gccagaggcg ttgcgtacat accattggtt gatgttatcc ggcaacatac
     1981 ggaacggcgt gcctgcctgg acatacactt ttttcacccg cccctggttg aggaagtcgt
     2041 tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt gatatcagac agcgccacac
     2101 ccatagtctc ggctttcgca gcgttgacgt tcactttgaa catcggcgta tcttccaggc
     2161 cgttcgggcg taccccgatg acctgattcg gtgattgcgc tgccagtgat aacagctcgt
     2221 ttcgcgcctg ggtcagcttt tcgtgcccca gattgccgtt gtccagcagt tccatatcaa
     2281 aacctgacgc ggtacccagt tcagccaccg cgggtaagtt gaacgggaag acgacggctt
     2341 tattgatact gcttagcgca atcattgccc gctgaataat cgcggtaacc gagttttcct
     2401 cgccgacacg ttcagaccac ggcttgaggc tgataaacgc cagaccgttg ttttgcccct
     2461 gaccactgaa gccaaagcca ccaacggtaa acaccgactg aacattattt ttctcttttg
     2521 tcagataata atccgtcacc tgttgtagca ctttcgtggt gttaaccatg gttgcaccgg
     2581 aaggtaactg cgcggtggtc ataaataccc cctgatcctc ttctggtaag aaagaggtcg
     2641 gcgtacgcag gaacagcacc gccatcccgg cgcaaatcag caggtagacc accatgtagc
     2701 gaccggtaca acgcaacagc gagcgggtgc tgtcggtata gtgctgagtt gatttttcaa
     2761 acagcgtgtt gaagcgtgcg aacagggtgt taggtttgtg accgccctcc ggcgcggctt
     2821 tcagaatggt ggcgcacagg gcaggggtca ggctcattgc tacaaatact gaaagcagca
     2881 tggaggagat cagcgtgatg gagaactggc ggtagatctc cccggtcgca ccgctcataa
     2941 aggccatcgg cataaacact gcggaaagaa caacggcaat cccgaccagc gcacgttgga
     3001 tctgccccat cgatttatgc gtcgcttcct tcggcggtag cttatcttcc gcaatgacac
     3061 gctcgacgtt ctccaccacc acgatggcgt catccaccag taaccctatc gccagcacca
     3121 tcccgaacat agttaacgtg ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc
     3181 cgagaataac caccggtacg gcaatcgtcg ggatgattgt ggcgcggaaa ttctgcaaaa
     3241 acagatacat gaccaggaag acgaggatga tagcctcaac cagcgttttg aaaacttcct
     3301 gaatagaaat tttgataaac ggtgtggtgt cgtaaggata aaccgtcttc aggcttgcag
     3361 ggaaataagc cgataaacgg ttcagctcct ctttcaccgc tcgcgaggta tccagggcgt
     3421 ttgctccggt agccagtttg atggcgatcc cggcagcagg tttgccgtta tagcgcgcca
     3481 cggtggaata atcttccgcc ccaagttcga cgcgggcgac atcacgcagc agcacttgcg
     3541 aaccatcttg ctgaactttc aacaggattt tgccaaattc ttccggcgtt tgcagacgcg
     3601 tctgcacaat gatcgaggcg ttcagttgct ggtctgccgc ctgtggcatg ccacccagtt
     3661 gaccaccgga aatctggttg ttttgcacct taatctggga aataacatcg gaaggtacca
     3721 ggttatattt attgagtttt tgcgggtcca gccagatacg catggcgtac tcggaaccaa
     3781 agagctgtac gctaccaacg cccgcggtac ggcttagcgg gtctttgata ttagacgcta
     3841 catagtccgc gatatcgtac tggttgaggc tgccgttatc agaaataaat gccgctacca
     3901 tcaggatatt actgctcgac ttatcgacgc taattccctg ctgctgcacc gcttcaggta
     3961 atgaaggcat agcgagttgc agtttatttt gcacctgaac ctgtgcgata tcaggtgatg
     4021 tccccgtctc gaaggtcaga gtgatggagg cattacctgc cgcatcgctg gttgaagaca
     4081 tgtacatcag gccatcaagc ccattcatat tttgctcaat cacctgagtg accgagtctt
     4141 ctaccgtttg cgcatcggca cccggatagg tagcgctgat ggtaatggtc ggtggcgcaa
     4201 tctgcggata ctgcgcaacc ggtaagttca tgatcgccag accgcctgca agcatcataa
     4261 taatggcaag tacccaggca aaaaccgggc gatcaataaa atagttagcc atgaaagtcc
     4321 ccttaagcct gcaacgttat tgtttcgatt cggtgctggc gttttcctgg ctggaggaaa
     4381 ttgcacgtgc tttgataccc ggacgaatgc gttgcaaacc ggaaacgatc acccgatcgc
     4441 cagcctgcaa gccagaggtg acaacccact ggtcgccgat ggctttgctg gcttcgattt
     4501 cgcgtagctg cacgacatcg tctttatcca gaatgagtgc cgttgcttta ccctgggcgt
     4561 tgtgggtgac gccttcctgc ggcaccagta atacattctg gcggctacct tcatccacta
     4621 atgccgtgac atacatgcca ggcagcaagt caccatttgg gttggggaaa atcgcccgta
     4681 acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga gaatttcagc gtgccggtct
     4741 ggccgtaacg tttaccattt tccagattga gctgtactgg cgtactgccc tgaacctgtt
     4801 tgatttgccc actggcgacc tcttctttca tgcgcaagaa atcctgcacc gactgcgtga
     4861 gatcgacata aatcgggtcc agacgttgta cggtaaccag cgaatctgcc tgattagcgg
     4921 taacgagtgc gccgacggtc accgacgatt tcccgctgac gcccgtaatc ggcgaggtga
     4981 cattcgcgta ttgcagattg atcgtcgcct gttcaacagc cgctttggcg acggtgacat
     5041 tggcttctgc ttcattcaac tgggtgcgtg cggtgtcgta atcctgacgg ctaacatagt
     5101 tggtcttcag caacgatgcc tggcggttaa aggtgatgcg ggcattgctg gcggtagaga
     5161 gcgctttcgc cagcgaacct ttggcggagt tcagctctgc ctgtaaaggc gcaggatcaa
     5221 tctgatacag cgaatcgccc tggttcactt tatcgccttc gataaagttg cgtttaatga
     5281 taataccgcc cacctgggga cgtatctcgg caacttcata aggaacggtt ctaccgggca
     5341 attcgctcaa cacattgacc gaaccggggg agagtgtgac gacaccgacc tcaggcatca
     5401 tggcggcggc gttttccgcc gatttgtcat cgcaggcggt gagcatcgcg ccgcagaata
     5461 acaacggtat taacagtttt cttcttctgt tcattttagt ccctgaaaat tcttgagaaa
     5521 taacggttct ttaaattgcg cgcgaggaga gatatgaggg atttacagac cagcggcata
     5581 gtggggttgt cagccagtaa gatgggatgc aagcacagtg atcgacatgg tgaggtcaac
     5641 gacaaaaaaa atgttgccgt tctgccaaca gtttctgcca gcatgcgggc aacgaagggt
     5701 tctactggtg gatacacata ccaggggaat aaagtcgttt tcgtctctcc ttcaataatc
     5761 accgggggta ttgaaatcag catctgcatc cctcgtcatg ccagccatca atttcagttg
     5821 cttatgtcct tactaaaaat aagatgtgat acccagggtg acgatgtcgc
//
LOCUS       DAZFRG010000270         5792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFRG010000270 DAZFRG010000000
VERSION     DAZFRG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.272"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(307..1433)
                     /gene="intE"
                     /locus_tag="ACHKUC_004301"
                     /pseudo
     CDS             complement(307..1433)
                     /gene="intE"
                     /locus_tag="ACHKUC_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1414..1551)
                     /locus_tag="ACHKUC_004302"
                     /pseudo
     CDS             complement(1414..1551)
                     /locus_tag="ACHKUC_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003841900.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            1626..2435
                     /locus_tag="ACHKUC_004303"
                     /pseudo
     CDS             1626..2435
                     /locus_tag="ACHKUC_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2762..3838
                     /locus_tag="ACHKUC_004304"
     CDS             2762..3838
                     /locus_tag="ACHKUC_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HFO6620444.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTIRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLDVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSRIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQLNSIPITA"
     gene            3855..4163
                     /locus_tag="ACHKUC_004305"
                     /pseudo
     CDS             3855..4163
                     /locus_tag="ACHKUC_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4185..5072
                     /locus_tag="ACHKUC_004306"
                     /pseudo
     CDS             4185..5072
                     /locus_tag="ACHKUC_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774771.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            5069..5770
                     /locus_tag="ACHKUC_004307"
     CDS             5069..5770
                     /locus_tag="ACHKUC_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6620445.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTSL
                     YQNMRANALTDTELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGVMK"
BASE COUNT         1556 a         1343 c         1441 g         1452 t
ORIGIN      
        1 aacgggttat gtgtttggct cttatgtcag acttgtagta atgcttgaag tttttatttc
       61 tgttctaatt tcgtagttcc attttattaa atagatacca agctctatgc ataagagctt
      121 ggtatctatt gaaattaagc tcaataactg atctgctctc cattgattaa cacgtcacaa
      181 tattagtaat gtaactatta ggtttcgaga ttaactgcga acttctgctt tttgctcaaa
      241 gtggactgtt tggttctttt tcaagtgtag ttcgaaattc aaggtggcgt taaggcactt
      301 aatgctttac aacgctactt tactccatcc tttacctcga tcatcatgat aacgatcggt
      361 ttgctgttgc gttttatgac caagtagttt ttgtgtattt aaaccctgtt ctttatacaa
      421 acgttctgat aaagaccttt gttcatgaaa tgttgcggga gaaccttcac cccagttaat
      481 tcctgctaaa tccctcgctt tactgaaatt catcgtcaat gtattggctt taacctgcgc
      541 gccgcgttca gcctgtgagg tagtacgaaa aaaatgtaca aggtatgggc tgacagcata
      601 gtcacggcag cgggctacta catcgcgcaa actccattta atcgcattga gacgcaggga
      661 aagcgggatg gcgattttgc tcccggtttt ttcctggatg acatggagat gatcatccca
      721 aatatcgcta aatttcatac gcgagatatc acctaacctc tggccggtaa ccagtgctaa
      781 cagcatggca ttccccatat aacgatgact ggcatcagcg atatcaaaga ttttttgcca
      841 ctcttcaaga cttaaccgtt gtcgggtaat ttttcttctg ggctgtttgg tggctaatgc
      901 cgggttatag ccagggggac ttctccgtag tgttgcgcct ctttgaaaac atcaatcagt
      961 acggaacgaa ctacttgcgc cattctcggc tgtccggcgg cgatatactc gtcaagcaac
     1021 tgggctatat ccctgacatc aacggctgaa attaacttca gtcctgctcg ttccctgagc
     1081 aaggatactg gtttggcttt ctgtttatag gtgttgagct ttatatcgcc acttttcagt
     1141 ctgtcttcct ggattgcttg atagcgatct aaccaggttg acgttgtgat tgcttttcct
     1201 ttgctggttg cgatcctgtc actgatagct agaatctgcc gggttctttg ctcagctagg
     1261 cgtgtattgg cctcagtggc aatagcaatg gcttcagctt cgtttgtgcc caaagaatga
     1321 aacttcccgg tcactggatg cttatatcgc caataaactt tatttacttt tctgctatag
     1381 agcggataca agttcgggac tgaaacatta ttcttacgtg gtctggctgc cattacttaa
     1441 aatcctttgt agaataatag aatcactttt cttaatgaca ggagtaacta attctccaac
     1501 taactctgcg tcctcacgta ctcgccatag tcgaccttgt ttcattgccg gtgtgtagtg
     1561 gcacactgaa tttggccacc tgaacagagg tgatatgctc acctcagaac aacacaggtg
     1621 ctccaatgaa aaaaagaaat ttcagcgcag agtttaaacg cgaatccgct caactggttg
     1681 ttgaccagaa ctacacggtg gcagatgccg ccaaagctat ggatatcggc ctttccacaa
     1741 tgacaagatg ggtcaaacaa ctgcgtgatg agcgtcaggg caaaacacca aaagcctctc
     1801 cgataacacc agaacaaatc gaaatacgtg agctgaggaa aaagctacaa cgcattgaaa
     1861 tggagaatga aatattaaaa aaggctaccg cgctcttgat gtcagactcc ctgaacagtt
     1921 ctcgataatc gggaaactca gagcgcatta tcctgtggtc acactctgcc atgtgttcgg
     1981 ggttcatcgc agcagctaca gatactggaa aaaccgtcct gaaaaaccag acggcagacg
     2041 ggctgtatta cgcagtcagg tacttgagtt gcataacatc aggttctgcc ggggcaagaa
     2101 gcatcgccac aatggcaacc ctgagaggct tcagaatggg gcgctggctt gccggcaggc
     2161 tcatgaaaga actgggactg gtcagttgcc agcagcctgc gcaccgttat aaacgaggtg
     2221 gtcgtgaaca tgtcactatc ccgaatcacc ttgggcggca gttcgcagtg acagagccaa
     2281 atcaggtatg gtgcggcgac gtgacgtaca tctggacggg gaaacgttgg gcataccttg
     2341 ccgttgttct cgacctgttt gcaaggaaac cggtaggttg ggcaatgtcg ttctctccgg
     2401 acagcagact gaccatcaaa gcgctgaaaa tggcctagga aatccgcagt aaaccagccg
     2461 gggtaatgtt ccacagcgat agtaataatg ccggtatcag tttttatcat cactctgttt
     2521 gctgtttaac cagactggtg tgattactga tgcagtgaag accttcccgc atcctgactc
     2581 acacagcgat cgaccctttg tgtcctgccc tggacctgtc ggttgccgga agcgccttca
     2641 tgcgaggcgt ctcctcaccg atgcgcgtga ctcaagaagg gcctgacggt ttgtctcgtt
     2701 actgtcctgt ccgggttatc tgtctggaga ttcaactctg tttcctcaca ggagctctgt
     2761 tatggcaggt aaagttacgg aaaccgctgt tgtgggtggc gtggatacac ataaagatct
     2821 gcacgttgcc gctgtcgtag atcagaacaa taaagttctg gggacccagt ttttctccac
     2881 aatacggcaa ggttaccggc agatgctggc atggatgact tcgtttgggg cattaaagcg
     2941 aattggtgtt gagtgtactg gcacctatgg atcaggtctg cttcgctatt tacagaatgc
     3001 cgggttagac gttcttgagg tgactgcgcc agatcggatg gagcgacgca aacggggtaa
     3061 aagtgacacg attgatgctg aatgtgccgc tcacgccgca ttctcccgaa taagaaccgt
     3121 cacacccaaa acgcgcaatg gcatgattga gtctctgcgg gtattaaaaa cttgccgaaa
     3181 aacagcaata tcagcccgca gagtcgctct ccagattatc cattccaata ttatctctgc
     3241 cccggatgaa ttacgtgaac agctcagaaa tatgacgcgc atgcagctca tcaggactct
     3301 gggatcctgg cggcctgatg ccagtgaata ccgcaatgtt accaacgttt atcgtatttc
     3361 attaaagtcc cttgcccgac gctatctcga gttacatgac gaaatcgctg atttggatgt
     3421 catgattgcg gcaattgtcg atgagctggc gcctgaactg attaaacgta atgctattgg
     3481 atacgaaagc gcttcgcagt tgctgatcac ggcaggagac aatccccaac gattaagatc
     3541 agaatcaggt tttgcggcac tgtgtggtgt cagccctgtt cccgtatctt caggaaaaac
     3601 gaatcgttat cgacttaacc ggggtggaga tcgtgctgca aatagtgcac ttcacatcat
     3661 tgccatcgga cgtttgcgaa ctgacgataa aacgaaggaa tatgtcgcca gacgagtagc
     3721 ggaagggcat acaaaaatgg aagcaatacg ctgcctgaag cgctatatct cacgcgaagt
     3781 ttatacatta ctgcgtaatc aaaacaggca gctcaacagc atcccgataa cggcttgact
     3841 cttagaaggg cgtccagggc agccactata caagcaggca gttccggcag ttactgtggc
     3901 gttaccagat caaacagagt ctgagtcgac gaggaaattg ctgggataac agcccgatgg
     3961 agcgcttctt caggagtctg aaaaacgagt ggataccggt gacgggttac atgaacttca
     4021 gcgatgctgc ccatgaaata acggactata tcgttgggta ttacaacgcg ctcaggccgc
     4081 acgaatataa cggtgggttg ccaccaaatg aatcggaaaa ccgatactgg aaaaactcta
     4141 aagcggtggc cagtttttgt tgaccactac aaaacactgc tgagatattc aattttccag
     4201 tgccggatgt ggcacaaaag gagcggcgcg tggcagatct cgacgatggt tatacgcgca
     4261 ttgcaaatga gttgctggaa gctgtgatgc tggccggatt aacacagcac cagtttctgg
     4321 tcttcctggc tgtcatgcgc aaaacatatg gctttaataa aagactggat tgggtgagca
     4381 acgagcaact ttccgaattg accgggatat tgccgcacaa gtgttctgct gcaaaaagcg
     4441 ttctggtaaa gcgtgggatt tttattcaga gcgggcggaa tatcggcatt aataatgtgg
     4501 ccagtgaatg gtcaacatta cccgaatcag gtaagaaaaa taaagtttac ctgaaagagg
     4561 taaatttacc tgaatcaggt aagaaaagtt tacccaaatc aggtaaacac aaaagacaaa
     4621 ctaacaaaag acaatataaa acctttttcg tccgagaatt ctgacgaatc ctctgaccag
     4681 ccagaaaacg atcttcctgt ggtgaaaccg gatgctgcaa ttcagagcgg cagcaagtgg
     4741 gggacagcag aagacctgat cgccgcagag tggatgtttg acatggtgaa gaccatcgcg
     4801 ccatcagcca gaaaaccgaa tttgcagggt gggctaacga tatccgcctg atgcgtgaac
     4861 gtgacggacg taaccaccgc gacatgtgtg tactgttccg ctgggcatgc caggacaact
     4921 tttggtctgg taacgtgctg agcccggcca aacttcgcga caagtggacc caactcgaaa
     4981 tcaaccgtaa caaacaacag gctggcgtga cagccggaaa accaaaactc gacctgacaa
     5041 acactgactg gatttacggg gtggatttat gaaaaacatc gccgcacaga tggttaactt
     5101 tgaccgtgag cagatgcgtc ggatcgccaa caatatgccg gaacagtacg acgaaaagcc
     5161 gcaggtacaa caggtagcgc agatcatcaa tggtgtgttc agccagttac tggcaacttt
     5221 cccggcgagc ctggctaacc gggaccagaa cgaactgaac gaaattcgcc gccagtgggt
     5281 gctggctttc cgggaaaacg ggatcaccac aatggaacag gttaacgcag gaatgcgcgt
     5341 agcccgtcgg cagaatcgac cattcctgcc atcacccggg cagtttgttg catggtgccg
     5401 tgaagaagca tccgttattg ccggactgcc aaacgccagc gagctggttg atatggttta
     5461 cgagtattgc cggaagcgtg gcctgtatcc ggatgcagag tcttatccgt ggaaatcaaa
     5521 cgcgcactac tggctggtta ctagcctgta ccagaatatg cgggccaatg cgcttactga
     5581 tacggaatta cggcgcaagg ctgccgatga actgacttgt atgaccgcgc gaattaaccg
     5641 tggtgaggcg atacctgaac cagtaaaaca acttcctgtc atgggcggta gacctctaaa
     5701 tcgtgttcag gcgctggcga agatcgcaga aatcaaagct aagttcgggc tgaaaggagt
     5761 gatgaaatag agctaaagta ttagcttaaa aa
//
LOCUS       DAZFRG010000271         5755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFRG010000271 DAZFRG010000000
VERSION     DAZFRG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.273"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(131..3655)
                     /gene="nifJ"
                     /locus_tag="ACHKUC_004308"
     CDS             complement(131..3655)
                     /gene="nifJ"
                     /locus_tag="ACHKUC_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO6620446.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKHYDFLAQMAGKAEKSNTD"
     gene            3929..4195
                     /locus_tag="ACHKUC_004309"
     CDS             3929..4195
                     /locus_tag="ACHKUC_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO6620447.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4192..4614)
                     /gene="hslJ"
                     /locus_tag="ACHKUC_004310"
     CDS             complement(4192..4614)
                     /gene="hslJ"
                     /locus_tag="ACHKUC_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO6620448.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFNGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4725..5714)
                     /gene="ldhA"
                     /locus_tag="ACHKUC_004311"
     CDS             complement(4725..5714)
                     /gene="ldhA"
                     /locus_tag="ACHKUC_004311"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6620449.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1348 a         1488 c         1483 g         1436 t
ORIGIN      
        1 gcctttattg tcacagattt tattttctgt tgggccattg cattgctact gattttccaa
       61 cacaaaagaa gacaagcccg aacagtcgtc cgggcttttt ttagaattgg atgatcctta
      121 tccagagcat ttaatcggtg ttgctttttt ccgcttttcc ggccatttgt gccaggaagt
      181 catagtgctt ttgcaaatca gctgcagcat ctttccataa ctgttctgcc acttctggct
      241 gctgcgaatt cagacgacgg aaacgctgct cgtgaagtaa cgtttcttcc agtgcttctg
      301 acggcgggcg tgaatccagc gccagcggca gtttgccttc atcggcacga cgaggatcga
      361 agcgatataa cggccagaag ccggtagcag tgagttggcg catctgatcg tggctgagtg
      421 ccagatcgta accatgctct tcacatgggc tataagcgat gatcagcgat ggccccggat
      481 acgcttccgc ttcctgaatc gctttcaccg tctggttcag ctgcgcgccg agagaaatct
      541 gcgccacata aacatgaccg tacatcatca tactgacgcc aagatcttta cgcgctttac
      601 gtttaccatg ctcgccaaac ttagttactg cacccagcgg tgtcgctttc gatgcctgac
      661 caccggtgtt ggaatagcat tgcgtatcca gcaccagaat gttgacgttt tccgtcaaac
      721 tcaatacatg atccagaccg ccaaagccga tatcgtaagc ccagccatcg ccaccaatca
      781 gccagattga tttttccacc agtgcatctg cgtcacgcag cagttcatgt gcttcggcaa
      841 catcgttgag ttgctggcgt aaagctgcaa cctgttcacg acgaacctct ggcgtggcgt
      901 ctgatttcaa cgcagtcagt aattctgccg ggattttatc ggcaaattga tcgagcagac
      961 gcagcacgcg gacacggtgt tgatcgaccg tcaggcggaa accaaggcca aattcggcgt
     1021 tatcttcaaa cagcgagttc gcccatgccg gtccacgacc attggcatcg gtggtatatg
     1081 gtgttgaagg caggttaccg ccgtatattg aagaacagcc agtggcatta gcgatcaaca
     1141 tccggtcgcc atagagctga gtcagcaatt taatatacgg cgtctcgcca caaccggagc
     1201 aagcaccgga atattcaaac agcggtgtaa tcagctgcga tgtacgaata tcaatacgtt
     1261 ccagtttgct acggtcgatt tctggcaggt tgaggaagaa atcgtaattg attttctctt
     1321 cttcgacatg ttccaggcgc gacatcatat tgatggcttt aatctctgga ttctgacggt
     1381 ctttcgccgg gcaaacttcg acgcacaggt tacaaccggt gcaatcttcc ggagccacct
     1441 gcaagacata tttctgcccg cgcatatcac gcgatttcac atccagcgaa tgcaggctgg
     1501 caggggcgtt ttccatcgct tcaggcggta ccacttttgc gcgaatagct gagtgtgggc
     1561 aagcggcgac acagtggtta cattgggtac agagttcctc tttccagatg gggatctctt
     1621 cggcgatatt gcgtttttcc cagcgcgtgg tgcccatcgg ccaggtgccg tctggcggca
     1681 gcgccgaaac ggggagggcg tcgccaagcc cggcgagcat cgcagcggtt acggttttca
     1741 cgaaatcagg agcggcatcg gaaaccactg gcggtcgatt ggcgctatgc ggattaactg
     1801 gctgcagtgg aacttcttct acggattcac gcgccagcgc cagagcctgc cagttgcgtt
     1861 ccaccagatc ctggcctttg ctactgtaac ttttggcaat cgcaccctgc aattctgcga
     1921 gggcactatc gccaggcaga atctgcgtca gatggaaaaa agccatctgc atgacggtat
     1981 taatacgggc cgccaggcca cattcgcggg cgattttcgc cgcgttaatc acatagaagc
     2041 gcgctttttt ctggtttagc acggcctgaa cttcctgcgg caagcgcgac cacacttcag
     2101 ctgcgctgta cggcgtgttg agcaggaaaa tgccgccagg ttttaaacgc tcagccatct
     2161 gatatttatc gataaactgc aactggtggc agccaacaaa atcagcctgg gaaatgagat
     2221 aagcggaacg aatcggctgt tcgctcacgc gaaggtgaga aaccgtcagg ccgcccgcct
     2281 ttttggaatc gtaaacaaaa tagccctgtg cgtaccacgg cgtggaatta ccgataatct
     2341 tgatattgtt tttggtcgcg gaaacgctgc catcactacc aaggccataa aacaaggctt
     2401 ccagtttcgc cgagtttggt agggtgtttt ccggcaacgg cagtgacaga ttggttacat
     2461 catcgtaaat accaaccgta aagcgcgctt tcggtttagc cgcgttgagc tcggcaaata
     2521 ccgccagtac acagtccggg ccaaattctt tggatgaaag accatagcgc ccaccaatga
     2581 cacggggcag ggtttcgcgc tcgccattat taaaggcttc tgccagtgcg gtcatcacat
     2641 ccaggtacag gggttccgct tgcgcacctg gctctttggt tctgtccagt accgccacgt
     2701 tgcgcacgga tcccggcaga gcttgcagca agtgtttggc ggagaagggg cggtacaggc
     2761 gaactttcag cacgccgact ttttcgccac gggtcagcaa ttcatcaacc acttcttcac
     2821 tggtgccaat ggcagagccc atcaggataa tcacccgttc cgcttgcgga tgcccgtaat
     2881 actcaaacgg ctgatactga cgacctgtcg cggcagcgaa atcattcatc gcctgttcaa
     2941 catggtcata gaccgcgttg taccatgggt tggtggcttc gcgagactgg aaataagtgt
     3001 caggattggc ggacgtaccg cggatcaccg gatgttccgg gttgagtgcc cgggcgcgat
     3061 gagcatcaat ttctgcctgc ggcatcagtt caagaatcgt gtcatcggcc agcgggacaa
     3121 ttttattgat ttcgtgggat gtgcggaaac catcaaagaa atgaataaat ggcacgcggc
     3181 ttttcagcgt cgcgatgtgc gaaatgagtg caaagtcttg cgcctcctgg acgtttgctg
     3241 cacacaacat cgcgcaaccc gtctggcgca ccgccataac gtcggaatga tcgccaaaaa
     3301 tagagagtgc atgtgtggca acggtacgtg ccgcaacatg caggacaaac ggtgtgagtt
     3361 cgcctgccag tttgtacagc gttgggatca tcagcagcaa accctgcgat gacgtaaacg
     3421 atgttgagag ggcaccagtt tgcaaagcac catgcacggt agcgatagca cccgcttccg
     3481 actgcatttc aaccacgcgt ggtgtgtctc cccaaacgtt tttcaagccg tttccggccc
     3541 atgcatcagc ctgttctgcc atcgtggaac tgggggtaat agggtagatg gcgataactt
     3601 cactggtgcg aaacgccacc gaagcaaccg cgccattacc gtcaatagta atcatatgac
     3661 acccttacat tgcgcaaatg aggggcgcac gaaattgctg cgcgcccagt agtaatcgtt
     3721 caattttagc aaatggcttt cttctgcatt ttcgcttttg tgtcccccac atcagcgtaa
     3781 tgaatgtttt gatcaaacag agggcaaaaa aatagccata aaaaagtaaa aatgcggggc
     3841 aacgcgcagg acagctctcg acgccgccct ctgtgctgcc tattatttat ggcggtgttg
     3901 tttggcttga agatcagagg aagaaaagat gcgagcagcg ttttgggtag ggtgtgccgc
     3961 tttattgttg tcggcgtgta gtagtgaacc tgttcagcag gcgactgcgg cgcacgtagc
     4021 gccaggttta aaagcgtcga tgtccagtag tggagaagca aattgtgcaa tgatcggcgg
     4081 ttcgctttct gttgcccgcc aactggatgg tacggcgatt gggatgtgtg cattacccaa
     4141 cggcaaacgc tgtagcgaac agtcacttgc cgccgggagc tgtggcagct attaattcat
     4201 taaatccgcc agcttataag ttaatgtctg tttcgcggtc gccagcgtta actggttcgc
     4261 ggtcagatcc acttgtgcac cttctttcag catttcgcta atggtgttat cgagttcatt
     4321 aagctgcggg ttagcgcaca tcatacgggt cattgccagc cctttggcgg tcagttcacc
     4381 attagacagt ttgccttcac cgttaaagcg gttacacatg ctgccggaaa tcatcatctt
     4441 ttcaccaaag ctgatttctg gcggattttt atcgctggtc acgggcttac cgtttacgct
     4501 ttccagcaca aagcgatgat gctgtagctg ttctggcgtt acagcaattt tgtcactact
     4561 tacacatccc gccatcagca ggcttagcgc aacaagcgcg gctactttct tcattgtggt
     4621 tctcaattac tgtttctgat tcaggactat tttaagaata gaggatgaaa ggtctttggg
     4681 gattatctga ataagctccc caggaatgca ggggagcggg aagattaaac cagttcgttc
     4741 gggcaggttt cgcctttttc cagattgctt aagttttgca gcgtagtctg agaaatactg
     4801 gtcagagctt ctgctgtcag gaatgcctgg tgcccggtaa acagcacgtt gtggcaggca
     4861 gacaagcgac ggaatacgtc atcctggatc acgtcgttgg atttatcttc aaagaacaga
     4921 tcgcgttcgt tctcatacac gtccataccc aacgaaccaa ttttctgatt tttcagcgct
     4981 tcaattgctg cctgagaatc aatcaatgca ccgcgactgg tattgacgat catcacgcca
     5041 tttttcatct gatcgaaggc ggcttcgttc aacagatggt agttttccgg tgtcagcggg
     5101 cagtgcagag agataacgtc tgattcagag aacagggttg gcagatcgac atactccaca
     5161 ccgagttcca gcgccgccgc acttggatac ggatcgaacg ccagcagacg cataccaaaa
     5221 cctttcagaa tgcgcagcat cgccacaccg attttaccgg taccgataac gcctgccgtt
     5281 ttgccataca tagtaaagcc ggtcagacct tccagagaga agttagcgtc acgggtacgc
     5341 tgatatgcgc ggtgaatacg gcggttcagc gtcatcatca taccgatggc gtgttcagca
     5401 acggcctctg gatcataggc tggaacacgg actactttga gccccagttc ttttgccgcg
     5461 tcaaggtcga cgttattgaa accagcacag cgcaaggcga tatatttaac gccgtgcttt
     5521 ttcagctctt ccagcaccgg gcggctgccg tcatcgttta cgaaaataca taccgcttcg
     5581 cagccattgg cagttttagc ggttttttcc gtcagcagaa agtcaaaaaa ttccagctca
     5641 aagccaaagg actcgttcac ctgttgcagg tacttcttgt cgtactgttt tgtgctataa
     5701 acggcgagtt tcataagact ttctccagtg atgttgaatc acatttaagc tacta
//
LOCUS       DAZFRG010000272         5731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFRG010000272 DAZFRG010000000
VERSION     DAZFRG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.274"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..395
                     /locus_tag="ACHKUC_004312"
     CDS             <1..395
                     /locus_tag="ACHKUC_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620450.1"
                     /translation="GQAPEMVKKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFV
                     TSLAGFSPKQTTTINKDSVLQAARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNP
                     EDRAYDKVTVTVGADGNATGASVEFIGK"
     gene            425..634
                     /locus_tag="ACHKUC_004313"
     CDS             425..634
                     /locus_tag="ACHKUC_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6620451.1"
                     /translation="MPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIRNAEGQLRW
                     RCWNFEPDAGKQLNSYLASEGILRQ"
     gene            724..1542
                     /locus_tag="ACHKUC_004314"
     CDS             724..1542
                     /locus_tag="ACHKUC_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6620452.1"
                     /translation="MQLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDRHTSRSERY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGNEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1805..2284
                     /locus_tag="ACHKUC_004315"
     CDS             1805..2284
                     /locus_tag="ACHKUC_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6620453.1"
                     /translation="MKIVSQNTTTLSSATAPGNNQPQLVATPVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGKTWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2300..2776
                     /gene="radC"
                     /locus_tag="ACHKUC_004316"
     CDS             2300..2776
                     /gene="radC"
                     /locus_tag="ACHKUC_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6620454.1"
                     /translation="MQQLSFLLGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            2845..3066
                     /locus_tag="ACHKUC_004317"
     CDS             2845..3066
                     /locus_tag="ACHKUC_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6620455.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPCCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3146..3520
                     /locus_tag="ACHKUC_004318"
     CDS             3146..3520
                     /locus_tag="ACHKUC_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6620456.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            3567..3944
                     /locus_tag="ACHKUC_004319"
     CDS             3567..3944
                     /locus_tag="ACHKUC_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6620457.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCTHSQLINSIDILRARRATG
                     LMTRDNYRMVNDISQGKHPEAKQ"
     gene            3941..4090
                     /locus_tag="ACHKUC_004320"
                     /pseudo
     CDS             3941..4090
                     /locus_tag="ACHKUC_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            4166..4363
                     /locus_tag="ACHKUC_004321"
     CDS             4166..4363
                     /locus_tag="ACHKUC_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6620458.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLYQNR"
     gene            4448..5290
                     /locus_tag="ACHKUC_004322"
     CDS             4448..5290
                     /locus_tag="ACHKUC_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6620459.1"
                     /translation="MSEITAPRPEVVNGHTDVICSTSIRHILAVQKSTLLQIDTLTRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARETWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIINNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSVQGKECYEDEMFIIRYFKKGSAHIRFRKPELVDRLNDIIAKHYPG
                     VLPS"
     gene            5524..>5731
                     /locus_tag="ACHKUC_004323"
     CDS             5524..>5731
                     /locus_tag="ACHKUC_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620460.1"
                     /translation="MIRFSCRVYENVFLATDYQPSLKKTSLFFKLSRISCILSAPDTS
                     HPYMAFTCYKDEEVHKCDVKTIKEK"
BASE COUNT         1453 a         1520 c         1436 g         1322 t
ORIGIN      
        1 tcggacaggc gccggaaatg gtcaaaaaag ccgttcactc catagcagag cgtgatgtgt
       61 ccgttttaca ggcatttggt gtacgaatgg aattcacccg cggtgatatg ctgaaggtct
      121 gtccgaaatt tgtcacatca cttgccggtt tttccccgaa acagacgacc actattaata
      181 aagattccgt cctgcaggct gcccggcagg catacgcccg ggaatatgac gaggaaacaa
      241 cagaaaccgc tgattttggc tcttacgaag taaaaggcaa taaggttgag tttgaagtat
      301 tcaatcctga agaccgtgcg tacgacaaag tgaccgtcac ggttggtgct gacggtaatg
      361 ccaccggcgc cagcgttgaa tttatcggaa aatagccggt atgtaggact gccaccctgt
      421 tttattgccc gaaggccctt tctcacgcga acaggcggtt gccgtcacaa cagcttaccg
      481 caatgtgctt attgaagatg accagggaac gcatttccgg ctggttatcc gcaatgccga
      541 agggcagcta cgctggcggt gctggaattt tgaacctgat gccggaaaac agctaaattc
      601 gtatctcgcc agtgagggaa ttctcaggca ataaacgtct tcatttcatc catcaggccg
      661 cgtcttctcc gggagacgcg gccttttcgt ttataccgct aattcattca taaggagcaa
      721 agtatgcaat tagccagtcg ttttggttat gcgaatcaga tacgccgtga ccgtccgctg
      781 acacacgaag aactgatgca ctatgtaccc ggtatttttg gggaagaccg gcatacctcc
      841 cgcagtgaac gctatacgta tatccccacc atcaccgtgc tggaaagtct gcagcgggaa
      901 ggctttcagc cgttcttcgc ctgccagacc cgtgtgcgcg acccgggccg ccggggatac
      961 accaaacata tgctgcgtct gcggcgggcc ggagagataa acggagaaca tgtccctgaa
     1021 attattctgc tcaactctca tgacggtacc tccagctacc agatgctgcc gggttacttc
     1081 aggttcgtct gccagaacgg atgcgtctgt ggtcagtctc tgggggaagt gcgtgttccg
     1141 caccggggaa atgtggtgga gaaagttatt gaaggggctt acgaggtggt gggcgttttt
     1201 gaccggatag aggagaagcg tgatgccatg cagtcgctga ttctgccgcc accggcacgc
     1261 caggccctgg cacaggcggc actgacttac cgttatggta atgagcatca gcccgtcacc
     1321 accgctgata ttctgacacc gcgacgccgg gaggattacg gtaaggacct gtggagtgct
     1381 tatcagacca tacaggagaa tatgctgaaa ggcgggattt ccggccgcag tgcaaaagga
     1441 aaacgtatcc acacccgtgc cattcacagc atcgacaccg atattaagct caaccgcgca
     1501 ttgtgggtaa tggcagaaac gctgctggag agcatgcgct gataaaacat atccctgaag
     1561 gcgtgattca ggtaccagaa tcacccgacg tcccggagaa ctcagtctgt gcagactgag
     1621 tacaggcaat ggcatttggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1681 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtctcacggt cagtgatgaa
     1741 cgtgatgttg ccagctcccg gcctttattt cctctcattt atacacattg taaggatttc
     1801 actcatgaaa atcgtttctc agaacacaac tactctttct tcagccacag caccagggaa
     1861 taaccagccc caactggttg ctactcccgt ccctgatgaa caacgtatca gcttctggcc
     1921 gcagcatttt ggcctcattc cacagtgggt gaccctggag ccccgtgtct tcggctggat
     1981 ggaccgtctg tgcgaagact actgcggtgg tatctggaat ctgtacaccc tgaacaacgg
     2041 tggcgcattt atggcacccg aaccggatga agatgatgga aaaacctggg tactgttcaa
     2101 tgccatgaac ggtaaccgcg ctgaaatgag tccggaagcc gccggcattg cggcctgtct
     2161 gatgacgtac agtcatcacg cctgtcgtac ggagtgttat gccatgacgg tccactatta
     2221 ccggctgcgg gattacgccc tgcaacatcc ggaatgcagc gccattatgc gcatcattga
     2281 ctgaatggag gcagggacaa tgcagcagct ttcctttctg ctcggagaga tgacgcccgg
     2341 cgagcgcagt ctcattctgc gggccctgaa aaccctggac cgccatcttc atgaacccgg
     2401 cgtggccttc acctccaccc gtgcggcacg ggaatggctg attctgaaca tggcgggact
     2461 ggagcgtgaa gagttccggg tactgtatct gaacaaccag aatcagctga ttgccggtga
     2521 aaccctcttc accggcacca tcaaccgcac ggaagtccat ccccgggaag tgattaaacg
     2581 cgccctgtac cacaatgccg ccgccgtggt actggcacac aatcacccgt ccggtgaagt
     2641 cacccccagt aaggcagacc gccttatcac ggaacgtctg gtacaggcac tggccctggt
     2701 ggatatccgg gtaccggacc atctgatagt tggtggcagt caagttttct cctttgcaga
     2761 acacggtctg ctttaacccg tcaccgtcac aaccacatca cacctgtttt cacttttatc
     2821 ttctgtcttc agaggtatcc cattatgaaa atcatcaccc gtggtgaagc catgcgtatt
     2881 caccaacaac atccgacatc ccgtcttttt ccgtgctgta ccggtaaata ccgctggcac
     2941 ggtagcacgg atacgtatac cggccgtgaa gtgcaggata ttcccggtgt gctggccgtg
     3001 tttgctgaac gccgtaagga cagttttggt ccgtatgtcc gtctgatgag cgttaccctg
     3061 aactgaatca gaaagggcat tctgatgagc cgaattatcg ccaccatcga accattctta
     3121 actgattttt tgtgaggatt ttatcgtgtc agacacacac cccgggacaa cgcatcccga
     3181 caacaacaac gaccgcccct ggtgggggct cccctgcacc gtgacgccct gtttcggggc
     3241 acgtctggtg caggagggca atcagttgca ttaccttgcc gaccgcgccg gtatcagggg
     3301 gcagtttagc gacgcggatg cgtatcatct ggaccaggcc tttccgctgc tgatgaaaca
     3361 actggaactc atgctcacca gcggtgaact gaatccccgc catcagcata ccgtcacgct
     3421 gtatgccaga gggctgacct gcgaagccga caccctcggc agctgtggtt acgtttatat
     3481 ggctgtttat ccgacactgg cacccgcaac cacctcataa ccccgaatat ctcaccttta
     3541 gcactaccag ttacagagaa cttaagatga aaacattacc tgacacacac gtacgggagg
     3601 catcgcgctg cccgtctccc gtcaccatct ggcagacact gctcacccga ctgctggatc
     3661 agcactacgg ccttacgctg aatgacacac cgttcgctga tgaacgtgtg attgagcagc
     3721 atattgaggc aggcatttca ctgtgcgatg cggtgaactt tctcgttgaa aaatacgcgc
     3781 tggtacgtac cgaccagccg ggattcagca cctgtaccca ctctcagtta ataaacagca
     3841 tcgatatcct ccgggcccgc cgggcgacag gcctgatgac ccgcgacaat tacagaatgg
     3901 taaatgacat tagccagggg aagcatccgg aggcgaaaca atgaaactgg ccctgacgct
     3961 ggaggcagac agcgttaacg tgcaggcgct gaacatgggg cgcattgtcg ttgacgtcga
     4021 tggtattaag ctcgctgaac tgattaacaa agtcgctgaa aacggttatt cactccgcgt
     4081 ggtggatgaa catgacgcta ccgaaacacc ggcaacttct gccagccctc atcagttgcc
     4141 ggaagacaat acagtgatac agagcgtgat gccattaacc actgaaacag cgctggatat
     4201 cctcattgcg tggctgcagg acaatattga ctgcgaatcg gaaattatct ttgacaacga
     4261 tgaggacaaa acggattcgg cagcactgtt gccctgtatt gaacaggcca gggaggatat
     4321 ccgtaccctg cgccaactgc agcttctgta ccagaaccgg tgagtcttac tcaccagact
     4381 tcattccact gacgccagcc tgaacagggc tggcgttttc atttatctgc aaaaaggaat
     4441 atcgattatg tctgaaatca cagccccccg tccggaagtg gtcaacgggc atacggacgt
     4501 tatctgctca accagtatcc gccacattct ggctgtacaa aagagtacgc tgctgcaaat
     4561 cgacacactt acccggcaac tggcagaaat ctcagcaatg acagaaagta ttggcggtaa
     4621 aaccgcactg gactgggcga tgaaacagga ttttcgctgt ggttgctggc tgatggaaaa
     4681 acctgaaacg gcaatgaaag ccatcacccg caatctcgat cgcgaaatct ggcgtgacct
     4741 gatgcaacgt tccgggatgc tgtcattaat ggatgcgcag gcccgtgaga catggtaccg
     4801 gtcactggaa tacgataatt ttccggaaat cagcgaagcg aacattctga gcacatttaa
     4861 acaactgcac cagaacaaag atgaagtgtt tgagcgaggg gtgatcaacg tcttcagggg
     4921 actgagctgg aattacaaaa ctaattgccc ctgtaaattt ggcagtaaaa ttatcattaa
     4981 caacctggta aggtgggacc ggtggggatt tcatcttatc accgggcaac aggctgaccg
     5041 actcgccgat ctggaaagaa tgttgcacct gttcagcggt aaaccgatcc ccgacaaccg
     5101 ggaaaacatc accattcatc tggatgatca catccggtct gttcagggta aagagtgcta
     5161 tgaagatgag atgttcatca tcagatactt taagaaaggc tccgcgcaca tcaggttcag
     5221 gaaaccagaa ctggttgaca ggctgaatga tattattgcg aaacactatc cgggagtgtt
     5281 accttcataa taaagctcta agaattgatt cgattgtgta ttttagcttc attcccttac
     5341 acaaagtcat ttgggggttc ttatgggggt tactcattta tccaacacag attaatcatt
     5401 taaaatcaaa acattaacac tcatattcga ctcctgtgat cgccaaaatg cctcttcagt
     5461 gatccccatc ctgcttattt ctgagcaacc tgaccctgct gatgtatctg gtagtatgac
     5521 agagtgattc gtttttcttg ccgagtgtac gaaaacgtct tccttgccac cgactatcaa
     5581 ccgtcactca aaaaaacctc tctttttttc aagctatcaa gaataagttg cattttatct
     5641 gcaccggaca ccagccatcc atatatggct tttacctgct acaaagatga agaagtgcat
     5701 aaatgtgatg ttaaaacaat caaagaaaaa t
//
LOCUS       DAZFRG010000273         5704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFRG010000273 DAZFRG010000000
VERSION     DAZFRG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.275"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..1395
                     /gene="adeQ"
                     /locus_tag="ACHKUC_004324"
                     /gene_synonym="yicO"
     CDS             61..1395
                     /gene="adeQ"
                     /locus_tag="ACHKUC_004324"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO6620461.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALCVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            1448..1900
                     /gene="yicN"
                     /locus_tag="ACHKUC_004325"
     CDS             1448..1900
                     /gene="yicN"
                     /locus_tag="ACHKUC_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO6620462.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2111..3301
                     /gene="nepI"
                     /locus_tag="ACHKUC_004326"
     CDS             2111..3301
                     /gene="nepI"
                     /locus_tag="ACHKUC_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO6620463.1"
                     /translation="MSEFIAENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3342..3635)
                     /gene="yicS"
                     /locus_tag="ACHKUC_004327"
     CDS             complement(3342..3635)
                     /gene="yicS"
                     /locus_tag="ACHKUC_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO6620464.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3857..4675
                     /gene="nlpA"
                     /locus_tag="ACHKUC_004328"
     CDS             3857..4675
                     /gene="nlpA"
                     /locus_tag="ACHKUC_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO6620465.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4679..5602)
                     /gene="yicL"
                     /locus_tag="ACHKUC_004329"
     CDS             complement(4679..5602)
                     /gene="yicL"
                     /locus_tag="ACHKUC_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO6620466.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1345 a         1294 c         1481 g         1584 t
ORIGIN      
        1 ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat ggacaaaaaa
       61 atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc gcgattattt
      121 aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat gaccacattt
      181 ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc acaaatggac
      241 ccaaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat tgcgatgggg
      301 gtatttgcta acttacccgt ggcactggct ccggcaatgg ggctgaacgc cttctttgcc
      361 ttcgtggtcg tgggggcgat gggtatctcc tggcagaccg ggatgggcgc gatattctgg
      421 ggcgcagttg ggctattttt gctcacgctg tttcgtatcc ggtactggat gatctccaac
      481 attccattaa gtttacgtat tggtatcacc agcggaatcg gattatttat cgccttaatg
      541 ggattaaaaa atactggcgt tattgtcgcc aataaagaca ccctggtgat gattggcgat
      601 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca
      661 tcacgtcatt ttcatgccgc ggtgcttgtt tctattgtgg tgacgtcttg ctgtggattg
      721 tttttcggag atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc
      781 attggtgaag tggatttgag cggtgcgtta tcacttgaac tcgccggtat cattttctcc
      841 tttatgctga tcaacttatt tgattcatca gggacattaa ttggtgtgac tgataaagct
      901 ggcttaatag atagtaacgg taaattcccc aatatgaata aggcgctgtg tgttgatagc
      961 gtcagttcgg tggcgggtgc gtttatcggt acctcgtctg ttaccgccta tattgaaagt
     1021 acttcaggtg tggctgtcgg tggtcgcacg ggtctgacgg ctgtcgtagt cggcgttatg
     1081 ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
     1141 ggagcgttaa tctttgttgg cgtgctgatg acttcaagcc tggcgcgcgt taactgggat
     1201 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg
     1261 atcaccgaag ggattgcact aggctttatg tcgtactgca tcatgaaagt gtgcaccggg
     1321 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcaa ctctgtttgc actgaagatt
     1381 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
     1441 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1501 ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agcgatcggc tgaacatcga
     1561 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1621 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1681 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1741 acaaaaagcc cgccttgccg cgccgattac tgatgctcag gtcaggctgg cgctgggttt
     1801 tctgcgcgaa accgaacctg aaatgcagga tatcaatgct tttcagatgc gctataacgc
     1861 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1921 ctacagaccg cgcctgacct ggtgggtgga taagacgttc acaccgcatc cggtaatctt
     1981 tgccctgttc cgtttcgatc ttaactccag ctcaagcaac atcgtaaaag ttgcaaaaac
     2041 gttaaatgca tcacacattt caatgttaga ctggtcggct tttcccctga aacatgccac
     2101 gggtaacacc atgagtgaat ttattgctga aaaccgcggc gcgaatgcca tcacccgacc
     2161 gaactggtca gccgttttct cggtggcgtt ttgcgtcgcc tgtctgatta tcgttgagtt
     2221 tttgcctgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc
     2281 cgggcaatcg gtgaccgtga ccgcctttgt ggcgatgttt gccagtttgt ttattaccca
     2341 gaccattcag gccactgacc gccgctacgt tgtcattctg ttcgccgttt tgctgacgct
     2401 ttcctgtttg ctggtttcct ttgccaactc attcagtctg ctgttaatcg gtcgcgcctg
     2461 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     2521 accgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     2581 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     2641 taatgcggcg gcggcgatgg gtgtgctgtg tattttctgg attatcaaat cactgccttc
     2701 gctgccaggt gaaccttcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     2761 agtgatggcc gggatgatcg ccatctttat gtcctttgcc ggacagtttg cttttttcac
     2821 ttatattcgc ccagtgtata tgaatctggc gggattcggt gttgatggct taaccctggt
     2881 gctgctgagt tttggtatcg ccagctttgt cgggacatcg ctttcgtcgt ttattctcaa
     2941 acgttcggtg aaactggcct tagcgggcgc accgtttgtg ctcgccctga gcgcgctggt
     3001 actgacgctg tggggaagcg ataaaattgt tgctactggt gtggcgatta tctgggggct
     3061 gacctttgct ttaattcccg ttggttggtc aacgtggatc actcgctcat tggccgatca
     3121 ggcagaaaag gctggatcta ttcaggtggc cgttattcag cttgctaaca cctgcggtgc
     3181 ggcaatcggc ggttatgcgt tggataacat tggtctgact tcgccgctga tgttgtccgg
     3241 cacattgatg ttgctgactg cattgttggt gactgcaaag gtgaaaatga agaaatcctg
     3301 atatcaggta aagaagcgtt ggtcagtggt tgttgccgtt cttacatatc cgggcattct
     3361 accttaccca acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc
     3421 tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct
     3481 gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gcaataccgt ggttttctct
     3541 tttgccgact gtagagaact gaatggcgac tcggcctgag aaatggctgg aaaaatcagg
     3601 aatacggtaa tcatgagtag catcgttggc ttcatagcgg caaccttctg caaatgtgca
     3661 tcgaatgaag cataagtgta gtttgctttt gccgctctca tcatgctgta cgaaaggtgt
     3721 ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt tttattctta
     3781 tcgatagcgt ttcgtttttt aaactgcagc gaccttaccg ctatagtcag gtaatcatta
     3841 ataaaaggat aaaaaaatga aactgacaac acatcatcta cgggcggggg ccgcattatt
     3901 actggcagga gttctgctgg caggttgcga ccagagcagc agcgatgaaa agcatattaa
     3961 agttggcgtt attaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtagcaaa
     4021 agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga
     4081 tgcgacaaat catggcgagc ttgatgcgaa cgtcttccag catcgaccct ttcttgaaca
     4141 agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccccat
     4201 ggcgggctat tccaaaaaaa tcaaaacagt ggcgcaaata aaagaaggtg cgacagtggc
     4261 gatccccaac gacccgacta accttggtcg cgcgctttta ctgctgcaaa aagagaagct
     4321 aattactctg aaagagggaa aagggttatt acctaccgcg ttggatataa ccgataatcc
     4381 acgccatttg cagataatgg agctggaagg ggcacagttg ccacgtgtac tggacgaccc
     4441 gaaagtggat gtagcgatta tcagcaccac ttacatccag caaacaggac tttctccggt
     4501 taacgacagc gtatttattg aagataaaaa ttcgccgtat gtgaatattc tggtggcacg
     4561 ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga
     4621 agtcgctaaa gcggcagaaa ccatctttaa cggtggcgcg gtgcctggct ggtaattctc
     4681 actcatgccg cgccggacga tttatttttc tggcgcggcg gcgggaatcc attgaaatca
     4741 aaatcaccga tgacagaatc agcagcgttc ccagccagtc cggcaatgtg aaggtgatcc
     4801 ccagcaacaa caaagagagc agcgcgctac ttaatggttc tgcacagctt aaaatgctgg
     4861 cttttggacc accaattaat tgtgctcctt tcaggtacag actaaatgtc agggacgtac
     4921 caatgaccac caaataaaaa aacgccagaa tcaaactgcc gttaacgaca aagtttgtcc
     4981 cttgtctggc ataaaagggc agcaggatca gaccgccaat cagcatactc cagccgacga
     5041 ccggtaacgt gccatagcgg gcaatcagcg ttgagggata ggtggtatag aatgcagcag
     5101 caaatgccga ggcaatcccc cagaataacg cggcaggaga gatcgataat gacgtcggat
     5161 taccgtgtgt caccaataaa aaagtcccga tcagcgatgt caaaatagcg cagaaaacca
     5221 gaatgcccgg gcgggattta cgcaccagtg agaaccaggc gacgataata gtcggtgaga
     5281 ggaattgcag caccgttgcc gtggctgcgt tcgatttttc gatggttagc aaaaaagtga
     5341 gctgtacggt tagcgcgcca accacggaaa aaatcagtag gctaatggca tctttgtgat
     5401 tgttaataat agaaaagatt ttatcaccat gaacaaatga tagcgtcagc agaatcaaac
     5461 cggcgaatat caaacgcgtc atagtcaaaa actgcgacga catctggctt tgctccatga
     5521 tgtattgcgc gcagacccct gaacttcccc acaacacggc ggcaatcaga acgttcagca
     5581 tcccctttct ggtggaaccc atttttccct ctgcatactg ttttgtcttt tcatcaatat
     5641 acgttaagga taccactacc gatgacggtg agggagtaga gtaatcaatc agttttagtg
     5701 aatg
//
LOCUS       DAZFRG010000274         5686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFRG010000274 DAZFRG010000000
VERSION     DAZFRG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.276"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..161)
                     /locus_tag="ACHKUC_004330"
     CDS             complement(<1..161)
                     /locus_tag="ACHKUC_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620467.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGT"
     gene            complement(247..453)
                     /locus_tag="ACHKUC_004331"
     CDS             complement(247..453)
                     /locus_tag="ACHKUC_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6620468.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARITQSRP"
     gene            1371..1550
                     /gene="hha"
                     /locus_tag="ACHKUC_004332"
                     /pseudo
     CDS             1371..1550
                     /gene="hha"
                     /locus_tag="ACHKUC_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
     gene            complement(1883..2080)
                     /locus_tag="ACHKUC_004333"
     CDS             complement(1883..2080)
                     /locus_tag="ACHKUC_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620469.1"
                     /translation="MVICKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            2279..2848
                     /locus_tag="ACHKUC_004334"
     CDS             2279..2848
                     /locus_tag="ACHKUC_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6620470.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHSRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            complement(3023..3412)
                     /locus_tag="ACHKUC_004335"
     CDS             complement(3023..3412)
                     /locus_tag="ACHKUC_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO6620471.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPAIADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNHHELARKFGVSLQWVYSVVK
                     RVRKEELDRMQGKLFADEPDADTEKKE"
     gene            complement(3384..3833)
                     /locus_tag="ACHKUC_004336"
     CDS             complement(3384..3833)
                     /locus_tag="ACHKUC_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO6620472.1"
                     /translation="MIKQRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKRKGFRVKPAGKASSGLPLDNHPQSRKIRALWIEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLNTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            complement(3826..4041)
                     /locus_tag="ACHKUC_004337"
     CDS             complement(3826..4041)
                     /locus_tag="ACHKUC_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620473.1"
                     /translation="MTDESVVCAMFWYCFVGWCTAELHRRSGFYSRYSGAGYWISWSV
                     MFLCWPVALPLYVDYIGGAGKRSNDDD"
     gene            complement(4034..4261)
                     /locus_tag="ACHKUC_004338"
     CDS             complement(4034..4261)
                     /locus_tag="ACHKUC_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620474.1"
                     /translation="MITLSGNSRKLKACRISARYLFARAFFKNVRPGITIGVIAGREQ
                     VEKYMSGAWWNNDPVIAARNIHISWGDIQND"
     gene            complement(4258..4941)
                     /locus_tag="ACHKUC_004339"
     CDS             complement(4258..4941)
                     /locus_tag="ACHKUC_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO6620475.1"
                     /translation="MTDQDKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREAFGVNSIIHPGTYPGVGFYGNR
                     ERAALAVYTWEVLARQLKKARQQYISAQNKRIKTATRTSRGDQFAEGWVLAVISEIQS
                     FALTDDERELMQQWLEHKYPQTQTTRARKPGRSRNGDASRYAGFREGQNVRLHRPVSG
                     QEQQKLEAR"
     gene            complement(4938..5243)
                     /locus_tag="ACHKUC_004340"
     CDS             complement(4938..5243)
                     /locus_tag="ACHKUC_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620476.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLTEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVMYTGDTEQ"
     gene            complement(5253..5525)
                     /locus_tag="ACHKUC_004341"
     CDS             complement(5253..5525)
                     /locus_tag="ACHKUC_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620477.1"
                     /translation="MITPQDAEKRTREIVAEYIRECGCKTPNHIRLVLTKLISMAAQA
                     IVATNGKEAALQVLVNTLTHTAEHEVPYRMETTAEGGLHITVDRKH"
BASE COUNT         1323 a         1464 c         1509 g         1390 t
ORIGIN      
        1 gtgccttttt ctggcagaaa aaagtgggcc agggtcaggc ggtgataacc atgaagatgc
       61 ggagccccca ttccatgagc gacgataagc ctcgtcacgt ttgtcatcca tttctgatgc
      121 tggatgagca gcagctcaca ccggttttca agtttttgca taatattctc tcctttagcg
      181 cgcagcaaaa tgcctccggg ggccggatca tgcctttgct gcggttgaac ggataacggt
      241 caggaattac ggacgggact gtgtaatacg tgcctgcaac caggcttcca cttcactttt
      301 cagccatcgg gagctacggc ccagtttgat gggggcagga aaggcaccat ccttgatgag
      361 cttgtaaaac cacttatcgg tcaggccggt cagctgagtg ataaacgaca tgtcgaccat
      421 ctggtcatcc atcagtgaaa ctggggtatt catgaaaatg ttcctccgga tgttgttaat
      481 ctcaaccggc gttggtgatg aaaacaacgc accaccttcc ccggcataac aaccggggaa
      541 aaacgtaaaa aataatcggg aatgtgaccg tcaggagata aacgggggga ttgcggtggc
      601 tgagccgtac cggtatgtcg ggtgattgtt gcgaaacgat gcttccgcac gcgcacgtga
      661 taaaggaggg aggactttcg tgagacaagg ggaaaactgg cgttctgata attgcagagt
      721 tgtttttgaa ggcggttgtg gtgcatcagc ccgtgacctg tgggcaggcg acaggtatca
      781 ctggtgggat aagactgtct gattggtatt ataaatatat aagagagctc aaacacatga
      841 gttcatatat gtgaaataaa tctgtgagtg tgatcagcag accagtctac ccagtagcaa
      901 cagattgcgt tgtactcaca gggttatccc tgataactgg cgtataacct gcgtacataa
      961 aacgtacctg caatgcacct gaacagagtg cattaagcat ccgcctgagc ggtaaacgta
     1021 tccgatctgg tgagtgacag tctgagagca ttctcagtaa tcgccccctt gtatcctgga
     1081 aattggttaa gataacagcg attagatttg gcaaggtgta tgcagccagc agcttaacat
     1141 ctcagctaac tggctggtta ccttcaggtt gtcgcaatgg catcggacat ccggtgtcgg
     1201 ataactaaac gtagcgttaa ggcgatattg ctgagagacc ggttaagtgg ttgcacggga
     1261 gtaatgagtt tggggagggt accagaatgt cagtctggtg cggtatgctg gcagtatcac
     1321 gttgattaca gcataaagta acgagagacg aatggaagtt aaaaccagag cagttccgtc
     1381 gttgttcatc ccgggagacg ctggaaaaag tgatttccca cacacgttat aaacttaccc
     1441 ctgcggagct ggaagccttt aactctgcgg tcgatcaccg gctggcagaa ctgacaatga
     1501 acaaacttta cgatcgcgtg ccggcttccg tctggaaata tgtcacctga atcttgaatc
     1561 cgcgctacga tgactccggt gttatctgtg caccggaaga tcgggaacat taagcagtat
     1621 aacggcaaca aagaattcga aatcaaaagg gaatggtgta cagaggggca ggcgttcagg
     1681 cattaccggc ctgtatcttc tgaaggggac tcgtatttgt cggggctgag tattccgctc
     1741 gattcgatca cggattggcg gaatacctga ctgtgattga ttcaggtacc attttgcagg
     1801 gagccggtgc gttactgtct ggcacaacaa acgcagcggg tcgctctgcc ccgccgattc
     1861 ctgataatcc ctcctctgta acctactgca acagtacaaa tacactgacg cctgaaacgg
     1921 taaccagaat cagcaccagc agtaacgtca gtaaacagag cagacgatcg gtcttccggt
     1981 aataaccgaa ggggtcgaca gcgccacaat acgggcaaca tactgtatta tgagtaattg
     2041 cgttaccaca gtccctgcac ttctttttgc atatcacgat cgctccctct ccacgcctta
     2101 tccgtaacct gttttttaca ttaaaaaaac aacgccggga attcaatcgt cagtccggcg
     2161 atgaccgttc gggttatcac agagtgcctg agacagtgtc ctgccggagg tcacctcagg
     2221 ggctctgcgc attttttacg acgtggttat tccgtatgaa ccatacggag atttaaccat
     2281 gacctacaaa tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact
     2341 gaatgttcat tcccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc
     2401 agcaacggac gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc
     2461 ttaccagaaa cgtaagcatc gggaaggtaa acgcgtgcat cccacaaccc tgcattacgt
     2521 ctgggcccgg gagtttgggg agtgcaaagg taaaaaacac tatcacctga tgctgctggt
     2581 caaccgggat acctggtgtc gtgccggcga ttaccgcgct ccgggatcac tggccgggat
     2641 gattaaacag gcgtggtgca gtgccctggg agtggatgcc ggacgttatg acacgctggc
     2701 acattttccg gtcaggccgg cggtgtggct ggaacgcgat gatgacaccg gctttcagca
     2761 ggtgctggag cgtgctgact atctggcgaa ggagagtacc aaagcttacg gcaccggtga
     2821 gcgcaacttt ggctgcagtc gtggctgagc ccgacagact gattagactt gagataaatg
     2881 acagggaata agtgagatat ttatgggaag aactgggtca aaatgggaag agaccaggag
     2941 gaatatgcaa taaccttaca gtatgtgaaa atcagaccgg cccgaaaaaa aaacagaact
     3001 cgcgccagcc tgattgcaga tattactctt ttttctccgt atctgcatcg ggttcatcag
     3061 caaatagctt gccctgcatc cgatccagtt cttcttttct tacccgctta accacgctgt
     3121 agacccactg aagcgaaaca ccaaatttgc gggccagttc gtggtggttg cgtccgttaa
     3181 actccctgaa gatttcccgg tcgcgctgac tgaccttcca taccatgccc attgggaaat
     3241 aaacgttttg cccgccccag acctgcatca ttcggttcgc gacagcctga ccaatctggt
     3301 cggcaattgc gggctcaata tcaataatct cgcgaacggt ctcagaggta tgctgtgcca
     3361 gttccaccag gagttccggc cctttacttc gaaactgatt caggtcgctc atgttttact
     3421 cccgcagctc tgcgctgcca cttcttcagt ttctcaataa cactgcttgc ctgttcagta
     3481 ttgagccagc gcagggcgct gatgcccgtt tcccgcttga tccaccgcgc taatgcattt
     3541 tctgaacggt cacgaacaat gccggcagca gccatttcaa tccatagcgc acggattttc
     3601 ctggactgcg gatggttatc cagcggtaaa ccggagctgg cttttccggc aggcttaacg
     3661 cgaaagcctt tccttttcat ggattccagc acgcagttta gttgtgtggt atccattcct
     3721 ttggttgagg ctttaccggt cagcccctgt aacatctggc ggtaggtgtc ttcatccata
     3781 cccagttcat tacgggcaat atgaatgagc tggataagac gttgtttaat catcatcgtt
     3841 gctcctttta cctgcgccac cgatataatc gacatataaa ggaagcgcca caggccagca
     3901 caggaacatc accgaccagc taatccagta gccggcacca ctgtaacgtg aataaaaccc
     3961 tgaacggcgg tgcagttcag cagtacacca accgacaaaa caataccaga acatggcaca
     4021 tacaacagat tcatcagtca ttctgaatat ccccccaact gatatgaata ttacgggcag
     4081 caatgacagg gtcgttattc caccatgcac ctgacatgta tttttcaacc tgttcgcgtc
     4141 cggcaataac accaattgtg atccccggcc tgacgttctt aaaaaaggcg cgggcaaaaa
     4201 ggtatctggc agatattcgg caggctttta atttccggct gttacctgat agcgtaatca
     4261 tctggcctcc agtttctgtt gttcctgccc actgaccggg cggtgcagtc tgacgttctg
     4321 cccttcacga aaccccgcat agcgcgaggc gtcgccattg cggcttcttc ccggtttacg
     4381 cgccctggtg gtttgcgttt gcgggtattt atgttccagc cactgctgca tcagttcacg
     4441 ttcgtcatcg gtcagggcga aggactgtat ttcactgata acggccagca cccagccttc
     4501 agcaaactgg tcaccacggc tggtacgggt ggcagttttt attcttttgt tctgtgcgct
     4561 gatatattgc tgacgcgcct ttttcagctg gcgggccagc acttcccagg tgtacacagc
     4621 cagtgcagcc cgttcccggt tgccgtagaa ccccacaccc ggatatgtgc cggggtgaat
     4681 gatggagtta acaccaaatg cctcgcggat gatgttcatc aggcccagca tgtagcgcgg
     4741 tggacggagg ctgcctgtcg gccagtagtg actgatggtt tcatcaatat cactcatggc
     4801 gatgtcggaa tgtgtgatgc cgtgaacatc catcagttta cgggcacggc gcagtgccag
     4861 agcggcctcg tgagggttgc cggatgcggc cagcgccagc aactttttca gtttctcaat
     4921 gtgtttatcc tggtctgtca ttgttcagta tctccggtgt acatgacgcc cagccgtgcg
     4981 gcaaggcgtt ccagtttttt ctgtttgtgg aagtcaatca gccggtccat cccctgaagg
     5041 cgcaactgct ctgtcatgat ttccacgtct gccagctctg ccgcgaggtc actttcgctg
     5101 ccctgtccgt tcaggttgcg ggcggcactg gccgccagtt cggcggcctc ttctgtcagt
     5161 ttcagggcct gtgcatccgg cccgaaacgc tgcagagcca gacggtagag ggcggtgcgg
     5221 gtgagttcgg tgtttcgtgt catgtcgcgc cctcagtgct tccggtcgac ggtgatgtgc
     5281 aggccgcctt ctgcagtagt ttccatccgg tacggcacct catgctctgc cgtgtgggtg
     5341 agtgtgttca ccagtacctg cagggcagcc tcctttccgt tggtcgccac aatagcctgc
     5401 gcggccatgc tgattaattt ggttaatacc agccggatat ggttgggggt tttacaacca
     5461 cattcacgga tatattccgc gacaatttcg cgggtccgtt tttctgcgtc ctgtggggta
     5521 atcattgcgc gtcctccctg tcagggcgga agaattccat gacgggcacg tccgccgaaa
     5581 aatgctggct gcagtacgga cagaccaggg tgacacgtac tgcaggtacg tggtacttac
     5641 cggacatcac ggcgatggcg ctgtgaaaac gcagggctgt tatatc
//
LOCUS       DAZFRG010000275         5640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFRG010000275 DAZFRG010000000
VERSION     DAZFRG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.277"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..904
                     /locus_tag="ACHKUC_004342"
     CDS             59..904
                     /locus_tag="ACHKUC_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4015 family protein"
                     /protein_id="HFO6620478.1"
                     /translation="MEKKFFIDWNNEIPQEEFINNILADEDLVKIRSLVISRWGECYE
                     ESCQPIIDMFAEHAERFAHLESLFIGDMESEECEISWIKQGDYSRLYAALPNLKKLTI
                     KGTQDLELGEIAHDKLEHLEIISGGTPCNVFASLQNSQLPALKTLIVYIGVEDYGFDT
                     SLDIVMKLASKSLFPSLTHLGLVNSEEQNEIVRRVLESDILPQLEVLALSCGTLTDEG
                     AEHLLKHADRLSHLKLLDLYHHYLTTAMQKKLQKTLTCTVELSEALTPEVYDDEVYMN
                     PMFTE"
     gene            964..1950
                     /locus_tag="ACHKUC_004343"
     CDS             964..1950
                     /locus_tag="ACHKUC_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4014 family protein"
                     /protein_id="HFO6620479.1"
                     /translation="MITLIGHPESKRTFYFCQAASRLQEHINVVNYDQALTLPLNKSM
                     VKIDPPLHQETDIRLINTVSQNYITFLQKMANRREIRFINSPESIIHLLDKRHCKQRL
                     IEHAIPTPPILADDIRSYDQLIACMRSEKVYRVFIKPNFGSGAAGVLALSLHPGDNRQ
                     VLYCAIQVAGNRLVNSKHIIRYENSDDITLIVNTILQQENIIERWLAKARVGNKSYDL
                     HIVYVNGEIVWRVVRTSSQPITNLHLQNEAIRFADLNLPAEKVAEIDTLCLNAMKLYP
                     DVHIAGLDVLLTQNLTPYIIEINGQGDLIYQDTCEQNIIYQRQIQVMREHHV"
     gene            1943..2815
                     /locus_tag="ACHKUC_004344"
     CDS             1943..2815
                     /locus_tag="ACHKUC_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4013/SEN3800 family hydrolase"
                     /protein_id="HFO6620480.1"
                     /translation="MYNVTDEVPENTRTAIHSCDGEINMNDLIAREANVLFVVLDSLR
                     FDIAVQEQEAGNTPNINRYGRWIKCEAAGNFTWPSHHSMFSGFMPKPLEAAPGHSMLF
                     FPKDIGLGRLGPKNAFAFSEETWIKGLENKGYRTICVGGVSFFNNRSGMGKVFPAMFK
                     ESYWYPNFSCTVKESMDNQLTLINKKVNAEHPLMMYINIDTIHYPNHFYIENAKPGDT
                     VETHAAALHYIDARIEKLFDIFRQTGRETLVILCSDHGTCYGEDGKYFHSFNHPIVNT
                     VPYFHFVLDGKTHE"
     gene            2808..4046
                     /locus_tag="ACHKUC_004345"
     CDS             2808..4046
                     /locus_tag="ACHKUC_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HFO6620481.1"
                     /translation="MNNESIYQQYMYSYPHKTAYRELNNIAFADVKSRIYEHKTHLYI
                     HMPFCQSRCGFCNLFTCTGADNAFIDNYIEAIIVQARQMQLAPVDWASFTIGGGTPLL
                     LNVGQLSRLFRGVFDELQVDAQIFKAIETSPSDTTAEKVALLRQLAVNRVSIGVQSFN
                     DLELATLHRRHSTKNAHRALELLRTGNFPILNIDIIYGIPGQTAASLLASLNQALDYQ
                     PDELFLYPLYNMPRQPDMYSLYIEARDRLLHEGYTQTSMRRFVRVNNHSAVESCGFEN
                     SLALGAGGRSYLGNLHYCTPWSQNQPASRQIVADFIARQDKTLINHGYVLSQAEMKRR
                     FVIKNLFFYKGLSLRDYQRQFSSEVLSDFPLLGDFIKQDYCHQQDNRIQLTAQGMALS
                     DQLGPLFISPEVRRLETHKR"
     gene            4043..4897
                     /locus_tag="ACHKUC_004346"
     CDS             4043..4897
                     /locus_tag="ACHKUC_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HFO6620482.1"
                     /translation="MTLKQIQTLIYRGEIKSCNYRCGYCPFHKQDRNDKQQDEAALWR
                     MVEHLPALGSPLNVLITPYGEALRYRYYMAAMAVISRYSHVQAVGIQTNASFNLSALI
                     ATFQHHLGDLAKLRLWCSFHPSQVSKEVFLKQILAIKDAGIACCVGAVASVTDYDAFQ
                     WLRAHLPADIYFWLNANERDKRPTSDERNSQFQRLDPLYHLETSLWPANVAFCLGGKK
                     SVFMNAEGDLFACNISKIKIGNLYQQQLSSPACMARHCHCYLAYHLRQDIPALSQFDA
                     ERCFRIFP"
     gene            4897..5595
                     /locus_tag="ACHKUC_004347"
     CDS             4897..5595
                     /locus_tag="ACHKUC_004347"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFO6620483.1"
                     /translation="MICRLIALDLDGTLLNSKKMISPASVSALQSAQQVGVKIILATG
                     RSHSEALPYYQQLQLTGPMICCNGSYLYHPRQRQILRPLPLDIHKVERLRRWFSQQAL
                     QPHFYTYDDFVLQISLVHRKASLLRQAEEFARQELSLACSWSWHHQLDITQAGCEKGK
                     SLAWYAQQQNIALDEIIAFGDNDNDAGMLQMVGKGIAMGNGSFLAKASSNCVIGHNNT
                     DAIADFLYAQGFSS"
BASE COUNT         1517 a         1291 c         1319 g         1513 t
ORIGIN      
        1 aagccattga ctacccggta agatcttttc cactgcaaca cccacaagga atgagaaaat
       61 ggaaaagaaa ttttttatag attggaataa tgagatcccg caagaagaat ttattaataa
      121 tattttagct gatgaagact tggtaaaaat tcgctctttg gtcattagtc gttgggggga
      181 atgctacgag gaaagttgtc aaccgataat cgacatgttt gctgaacatg cagaacgctt
      241 tgctcatctt gagtccctgt ttatcggcga tatggaaagc gaagagtgtg aaatttcgtg
      301 gataaaacaa ggcgattatt ctcgtctgta tgccgcactg ccgaacctca aaaagctgac
      361 gattaaaggt actcaagacc tggaactggg agaaatcgcc cacgacaaac tggagcacct
      421 ggaaatcatc tctggcggta caccgtgtaa tgtgtttgcg tcattgcaaa actctcaact
      481 gcctgcgtta aaaacactta tcgtttatat tggcgttgag gattatggtt ttgatacatc
      541 gctggatatc gtgatgaaac tggcgtcgaa atccctcttc ccaagcctga cgcatcttgg
      601 tctggtgaac agcgaagagc aaaacgaaat cgtccgccgc gtgctggaaa gcgacattct
      661 gccgcaactg gaagtgttgg cactctcctg cggtacgctt accgacgaag gtgcagagca
      721 tcttctcaaa catgccgatc gccttagcca tctaaagttg ctggatttgt atcatcatta
      781 cctgacaaca gcgatgcaga aaaaactgca aaaaacactg acatgtactg ttgaattatc
      841 agaagcgcta acgcctgaag tctatgatga cgaagtttat atgaatccga tgtttactga
      901 ataaataaca tttttgggag agagtcatct ctcccattat ttatcacaga aaaggaattc
      961 ttcgtgatta cgctaattgg tcatcctgaa agcaagcgca cattttattt ttgccaggca
     1021 gcgtcacgct tacaggaaca tatcaacgtg gtgaactatg atcaagcgct taccctgccg
     1081 ctaaataaaa gcatggtaaa aatcgatccc cccttacatc aggaaacaga tattcgctta
     1141 attaatacgg ttagccaaaa ctatattact tttttacaaa aaatggctaa ccgaagggaa
     1201 atccgtttta ttaactcacc agaaagtatt attcatcttc tggataaacg ccactgtaaa
     1261 cagcgattaa tagaacacgc gatcccgaca ccgccgatat tagcggatga tatccgtagc
     1321 tacgatcaac tgatcgcctg tatgcgcagc gaaaaggttt atcgcgtgtt tattaagccg
     1381 aatttcggat caggggccgc cggggtgctg gctttgagtc tacatcccgg cgataaccgt
     1441 caggtgcttt attgcgccat ccaggttgca ggtaacaggc tggtgaacag taaacacatt
     1501 atccgctatg aaaacagcga tgatattacg ttgattgtga atactatttt acaacaagaa
     1561 aatattatcg aacgctggct ggcaaaagcc agagtgggca ataaaagcta cgatttacac
     1621 atcgtgtatg ttaatggcga aatcgtctgg cgggttgtca gaacatcatc acagccgatc
     1681 accaatttgc atctgcaaaa tgaagctatc cgttttgccg atcttaattt acccgccgaa
     1741 aaagtggctg aaattgacac gctttgtctc aacgccatga aactctatcc ggacgtacac
     1801 attgccgggc ttgacgtatt actcacgcaa aatttaacac cctatattat cgaaattaac
     1861 ggccagggcg atcttattta tcaggacacc tgcgaacaaa acataattta tcaacgccag
     1921 attcaggtaa tgagagaaca ccatgtataa cgtaacagac gaagtaccgg aaaatacccg
     1981 cacggcaatc cattcctgcg atggcgaaat caatatgaat gatttgatag ccagggaggc
     2041 gaatgttctg tttgtagtgc ttgattccct gcgttttgat attgcggttc aggaacaaga
     2101 ggcgggcaat acgccgaaca ttaaccgcta tggtcgctgg atcaagtgcg aagccgctgg
     2161 caattttacc tggccttcgc atcattcgat gttctctggt tttatgccta agccgctgga
     2221 agcggcaccg ggtcattcaa tgctcttttt cccgaaagat attggtttag ggcgtttagg
     2281 gccaaaaaat gcgttcgcat tcagcgaaga gacgtggatt aaagggctgg aaaacaaagg
     2341 ttaccgcact atctgcgtgg gtggcgtctc cttttttaat aaccgttcag ggatggggaa
     2401 agtgttcccg gcgatgttta aagagagtta ctggtatcct aatttctcct gcacggtaaa
     2461 agagagcatg gataatcaac tcaccttaat taataaaaag gtaaatgctg aacatccgct
     2521 gatgatgtat atcaatatcg acaccattca ttatcccaat catttttata ttgaaaatgc
     2581 caagcctggc gatacggtag aaactcacgc cgctgccctg cattatattg atgcgagaat
     2641 cgaaaaactt ttcgatatct ttcggcaaac cgggcgggag acgttagtta ttctctgctc
     2701 cgatcatggt acctgctacg gggaagacgg taaatatttc cacagtttta accatccgat
     2761 tgtcaatacc gtaccctatt ttcactttgt gttagacggc aaaacccatg aataatgaat
     2821 cgatttatca gcaatatatg tacagttacc cgcataagac cgcttatcgg gaattaaaca
     2881 atattgcttt tgcagatgtg aagtcgcgta tttacgagca taaaacacat ctctatattc
     2941 atatgccttt ttgccagagc cgctgcgggt tttgtaatct ttttacctgc acgggcgcag
     3001 acaatgcttt tatcgacaac tacatagagg cgattatcgt ccaggcccgg caaatgcaac
     3061 ttgcgcctgt agactgggcc agttttacca ttggcggcgg tacgccactg ctgcttaatg
     3121 tcgggcaact gtcgcgattg tttcgcgggg tgtttgacga attacaggtc gatgcgcaga
     3181 tttttaaggc cattgaaaca tcgccttccg ataccactgc cgaaaaagtc gcgttgttgc
     3241 gccagcttgc ggtaaaccgt gtcagtattg gtgttcagag ttttaacgat ctggaacttg
     3301 ccacattaca ccgccgccat agcactaaaa atgcccatcg ggcgctggag ctactgcgca
     3361 cgggtaattt cccgatactc aatattgata ttatttacgg tattcccggt caaacagccg
     3421 caagtttatt ggcgtcgctt aatcaggcat tagattatca acctgacgaa ctgtttctgt
     3481 acccactgta taacatgcct cgccagccgg atatgtatag cctctatatt gaagcccgcg
     3541 atcgcttatt gcatgaaggt tatacgcaaa cgtcaatgcg ccgttttgtg cgggtgaata
     3601 atcattcggc ggttgaaagt tgtggctttg aaaattcgct ggcgcttggc gcgggcgggc
     3661 gcagttacct tggcaattta cattactgca cgccgtggtc gcaaaaccag cctgcatcgc
     3721 gacaaattgt cgcggatttt atcgcccgac aagataaaac ccttattaac cacggctatg
     3781 tgctttcgca agcagagatg aagcggcgtt ttgttatcaa gaatctcttt ttctataaag
     3841 ggctttcgtt acgtgattat cagcgccagt tctcctctga ggtattaagc gattttcccc
     3901 tgctgggcga ttttatcaaa caagattatt gccaccagca ggataatcgc attcagctca
     3961 ccgcgcaggg tatggcgctt tcggaccagc ttggcccgct gtttatttcc ccggaagttc
     4021 gcaggctgga gacgcataaa cgatgacgct caagcagatc cagacgctga tttatcgcgg
     4081 cgagataaag tcgtgtaatt atcgctgtgg ctattgtccg tttcataaac aagaccgcaa
     4141 tgataagcag caggatgaag cggcattatg gcgcatggtt gagcatttac cggcgctggg
     4201 ttcaccgtta aatgtgttga ttacgcccta cggcgaagca ttgcgttatc ggtattatat
     4261 ggcggcgatg gcggtaatca gccgttattc ccatgtgcaa gcggtgggta ttcagaccaa
     4321 tgcctcgttt aatttatctg cgttaattgc gacttttcaa catcatctgg gcgatctggc
     4381 aaagctgcgg ctgtggtgtt catttcatcc ttcccaggtc agtaaagaag ttttccttaa
     4441 acagatactg gcaattaagg acgcagggat tgcctgttgt gtgggggccg ttgccagcgt
     4501 aaccgactat gacgcattcc agtggttacg cgcgcatctt cctgccgata tctatttctg
     4561 gctaaacgcc aatgagcgcg ataaaaggcc caccagcgac gaaagaaata gccagtttca
     4621 gaggttggac ccgctttatc atctggaaac cagcctctgg cctgccaatg ttgcgttttg
     4681 tctcggcggc aaaaagagcg tgtttatgaa tgccgaaggc gatctcttcg cctgtaatat
     4741 cagcaaaatt aaaatcggca atttatatca gcagcaactt tcatctccag cctgtatggc
     4801 caggcactgc cactgttatc tggcttatca tctgcgtcag gatatcccgg ccctctcaca
     4861 atttgatgct gaacgctgtt ttcggatctt cccttaatga tctgccggtt aatcgcgctc
     4921 gatctagacg gcacattgct gaacagcaag aagatgattt cccctgcgtc ggtttcggcg
     4981 ctgcaaagcg cacaacaggt aggtgtgaag attattctcg ccaccggacg ttcgcacagc
     5041 gaagccctgc cgtattacca gcaattgcag ttaaccgggc cgatgatttg ctgtaacggc
     5101 agctatcttt atcatccgcg ccagcggcaa atattgcgtc cgctgccgct tgatatccac
     5161 aaggtggaac ggctacgacg ctggttttca caacaggcat tacagccaca tttttatacg
     5221 tatgatgatt ttgttttgca gattagcctg gtacatcgca aggcttcgtt attgcgccag
     5281 gctgaggaat ttgcccggca agaattatcg ctggcctgta gctggtcctg gcatcatcaa
     5341 ctggatatta cacaggcggg gtgtgagaaa ggcaaaagtc tggcctggta tgcacagcag
     5401 caaaatatcg ctctggatga aatcatcgcc tttggcgata acgataatga tgcggggatg
     5461 ttgcagatgg tagggaaagg tattgcgatg ggcaatggta gctttctggc aaaagccagc
     5521 tctaactgcg ttatcggtca caacaacact gacgcgattg cggattttct ttatgcgcag
     5581 gggttcagtt catgatgcct cttatcaggt ctacaaaagc aaaaaaacct gccatcgctg
//
LOCUS       DAZFRG010000276         5636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFRG010000276 DAZFRG010000000
VERSION     DAZFRG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.278"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..648
                     /locus_tag="ACHKUC_004348"
     CDS             <1..648
                     /locus_tag="ACHKUC_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620484.1"
                     /translation="LIYQDPELQDSEIGVRREVNIPLPDSDNSYHADYVMRNFSGRGR
                     VDEVLLEMQGGGETSSTGEITRHIAAWADLEFPTNEILRQPVAANTIETNAWRRQQEQ
                     FLVKGNVVDQTGGKIVFAVGSLLYDYLHRRFRNANLRDLKAHNWTLCILAFKEDTSDE
                     PRPGPIPLIIDDSKTLFTNYSTFVRFLTDQGAPRPELFEGSFLRLDNSSVTIMPR"
     gene            complement(629..1558)
                     /locus_tag="ACHKUC_004349"
     CDS             complement(629..1558)
                     /locus_tag="ACHKUC_004349"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO6620485.1"
                     /translation="MKKKPLKQYKVSSFFAGIGGFDLGLEKAGMEVVFQCEINKFCQK
                     VLRKNWSKVPLHTDITRLNADEIPESNVWCGGFPCQDVSSANQGKRKGLEGARSGLFY
                     TYAKLIEERKPEWLIIENVPGLLNSHNGQDFKVVIDTLVEFGYGVSWRVLDAKYFGTP
                     QRRRRVYIVASLGDMRSARVLFEPGATRIVDKQGQGERANASGASATGLSKADIYSIQ
                     HASIGRKATAGPQAKGYRNDGETYTLDSRGSSDAVCAPDAAFRVRASTGISRELDSNR
                     FRAVGNAVAVPIVEWLGKRIVLVDQQVLNEALS"
     gene            complement(1750..1915)
                     /locus_tag="ACHKUC_004350"
                     /pseudo
     CDS             complement(1750..1915)
                     /locus_tag="ACHKUC_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1998..2372
                     /locus_tag="ACHKUC_004351"
                     /pseudo
     CDS             1998..2372
                     /locus_tag="ACHKUC_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611478.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2460..3673
                     /locus_tag="ACHKUC_004352"
     CDS             join(2460..2751,2751..3673)
                     /locus_tag="ACHKUC_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6620486.1"
                     /translation="MTKKTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKTGCTP
                     ETLRVWVRQYERDTGGGDGGLTTAERQRLKELERENRELHRSNNILRQASAYFGEGGV
                     RPPLEKIMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRCTRAQRDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWVRRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKA
                     YYASIGNNDLAA"
     misc_feature    2741..2857
                     /locus_tag="ACHKUC_004352"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            3844..5292
                     /locus_tag="ACHKUC_004353"
     CDS             3844..5292
                     /locus_tag="ACHKUC_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6620487.1"
                     /translation="MRIRSLDDAATTEETLLTLALTPLVKELKALECNKESQISKVKA
                     ALVKAVNEIAEPHQERFSSISRQIQTGFQHVFPALGVQLSVEMNPPELKIDNLLKQGS
                     GLLVKEAAGNSRIGQQGTGARRALFWAMLQVHNEISRQNEKREALLKSFREQLKKEVR
                     KNVKSENINLLKQQIDAIENGAPVPEDTDDPALPGYILLMDEPENALHPMAARAAQAH
                     LYELGKHPDWQVLLTTHSPYFINPLEDHTTIARMQRSTDGKSLSPRLYVADEANFSPD
                     EKDNLQALQLTDIGFAEIFFGSYPIIVEGDTEHAAFISAITKEKHEMSGKVSIVRARG
                     KAVLVPLIKMLNHFKADFGIVHDIDWPYRRDGSNNGSWTLNTIIRNEIIKCRNNGQKV
                     YHRWSVPDFERFLGGEELGKDKPYTAFNRISRDEKLKEKIQNLIINLFEGKCYDPDDF
                     EPDDDFNAQLMEQLKIWAKNNGESDNVRVMGC"
BASE COUNT         1611 a         1153 c         1323 g         1549 t
ORIGIN      
        1 ctcatttatc aggatcctga attacaggat tcagaaattg gtgtaagaag agaagtcaat
       61 attcctttac cagattctga taatagctac catgcagact atgtaatgag aaatttttcg
      121 ggtaggggaa gagttgatga ggtcctgctt gagatgcaag gtggtggaga aacaagtagc
      181 actggagaaa ttactcgaca tatcgctgca tgggctgatc ttgagtttcc tactaatgaa
      241 attctaagac aacctgtagc agcaaatact atcgaaacaa acgcatggag acgacagcaa
      301 gaacaatttc ttgtcaaagg gaatgttgtc gatcaaaccg gagggaaaat cgtttttgct
      361 gtcggtagct tactctatga ctatttacac agacgtttta ggaatgcaaa tctcagagat
      421 ctgaaagccc ataactggac attatgtatt ttagctttca aggaagatac atcggatgaa
      481 ccacgtccag ggccgatccc gttaatcatt gatgactcaa aaacactatt caccaattat
      541 tcaacatttg taagatttct aacagatcag ggcgcaccac gccctgaact attcgaagga
      601 tcatttttac gtttagataa tagttctgtt acgataatgc ctcgttgagc acctgctgat
      661 ctacaaggac aattcttttt cctaaccatt caacaatagg gaccgcgaca gcgttcccta
      721 ctgctctgaa tcgattagag tccagttctc gggaaatccc tgtagacgct cgtactcgga
      781 aggcagcatc cggcgcacat acagcatctg aacttcctct tgagtcaaga gtgtaggttt
      841 ctccatcgtt cctgtatcct tttgcttgag gtcctgcggt tgcttttcgt ccaatacttg
      901 catgctggat agaatagata tctgcttttg ataggcctgt agcagaggct ccggaagcat
      961 ttgccctctc tccttggccc tgtttatcaa cgattctagt tgctccgggt tcaaaaagga
     1021 ctcgagcgga gcgcatgtct ccaagacttg cgacaatgta cactcttctt ctacgctggg
     1081 gtgttccaaa gtattttgca tccaatactc tccacgaaac gccatacccg aattcgacca
     1141 acgtatcgat gacaactttg aagtcttgtc cattgtggct gttgagcagt ccgggaacat
     1201 tttcaataat aagccattca ggctttcttt cttcgatgag ttttgcatat gtataaaata
     1261 agccgcttcg ggccccttct agacctttcc gctttccttg gttagctgac gacacatcct
     1321 ggcaaggaaa accaccacac caaacgttgc tctctgggat ctcatcagca ttaagtcgtg
     1381 taatatcagt atgtagtgga acttttgacc aattctttct aagtactttc tggcaaaatt
     1441 tgttgatttc gcattggaaa acaacttcca tccccgcttt ctcaagacca agatcgaatc
     1501 caccaatccc tgcgaaaaac gaggatacct tgtactgttt cagtggtttc tttttcatat
     1561 cggctttttc atcacttttt gaaccaagga tcctaacgga aaccagcccc gatggccacc
     1621 ttttttctaa ttaaagccac tttcttggta tccaggcatt gattatgaaa taaactaact
     1681 gtaattatat acagtatatt ttaaatgcca ataaaagtgt acttctttgc gaaaagcctt
     1741 ctaatttgac tagactttcg catagtcaaa cgggctaatc cctttctcgc gacttacatc
     1801 cagaaaatcc actcaccatt gcaacatcag tcgctgcatc tttcaaatgt tccactttat
     1861 ggatataagc agcccgtact tttttacgtt cctgatgact catctgacgt ccaaccactc
     1921 gcgggactga cctgctccct gttgattaat acaccgcgat gttagtaatg tcttcataag
     1981 ctacatgagg acatcccatg aagaagcgtt tttccgacga acagatcatc agtatcctcc
     2041 tcgaagctaa agccggggtt tctgcccgtg agctctgccg cgcattggat caatgagcct
     2101 ttgagcatag tgtcgagttg cgtttaatcc agccgggcaa gccaacgcag aacggattta
     2161 ttgagagctt taacggacga tttcgcgatg aatgcctgaa tgagcactgg ttcagcgata
     2221 tcgttcacgc taggaaagtg attaatgact ggcggcagga ttataacgag tgtcggccac
     2281 attcagcact gaattatcag acgccatcag agcttgcagc actatggcga aatggaaaat
     2341 gtgaaggtaa ccaaaccgac attactaact gataattgta tctaatactg ggggcaggtc
     2401 aggattgaac cgccccggaa atcctggaga ctaaacttcc tgagaaagag gtaaacagga
     2461 tgactaaaaa aacacgtttt tcccccgaag tccgtcaacg ggcagttcgt atggttctgg
     2521 aaagtcaggg cgaatatgac tcacaatggg cggcaatttg ttccattgct ccaaagactg
     2581 gctgtacgcc ggagactctg cgtgtctggg ttcgccagta tgagcgggat accggaggcg
     2641 gtgatggtgg gcttaccacc gctgaacgtc agcgtctgaa agagctggaa cgtgaaaatc
     2701 gtgaactgca ccgcagtaac aatatccttc gccaggcttc cgcttatttt gcgaaggcgg
     2761 agttcgaccg cctctggaaa aaataatgcc actgctggat aagctgcgtg agcagtacgg
     2821 ggtcggaccg gtatgcagtg aactgcatat tgccccgtca acgtattacc actgtcagca
     2881 gcagcggcat catcctgata aacgctgtac ccgtgcgcag cgcgatgact ggctgaagag
     2941 agagatacag cgcgtatacg atgaaaatca tcaggtgtac ggtgtgcgta aagtctggcg
     3001 tcagttgtta cgggaaggaa tcagggtggc cagatgtaca gtggcgcgcc tcatggcggt
     3061 tatgggactt gccggtgttc tccggggtaa aaaggtccgc actaccgtca gccggaaaac
     3121 cgttgccaca ggtgaccgcg taaaccgtca gttcgtggca gaacgtcctg accagctgtg
     3181 ggtggctgat tttacttacg tcagcacatg gcagggcttc gtctatgtgg cgtttatcat
     3241 tgatgtgttt gctggataca tcgtggggtg gcgggtctca tcgtctatgg aaacgacatt
     3301 cgtgctggat gcgctggagc aggcgttgtg ggtccgtcgt ccgtctggca ccatccatca
     3361 cagcgataaa ggctctcagt atgtgtcact ggcctatacg gagcgactaa aagaagccgg
     3421 attactggca tcaacaggga gtacaggcga ctcgtatgac aacgcgatgg ctgagagcat
     3481 caatggtctt tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc gtgcagaagt
     3541 ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc tgggaaggct
     3601 gggccatact cctccggcag aagcagaaaa agcttattat gcttccatcg gaaacaatga
     3661 tctggcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg gttcagttta
     3721 tgggtttggg atgaaacggg aaaaccaaca agaaaaacat gggatcctca gactgaagac
     3781 tggtcagagg acagcaaggc cggtggagcc gataatgttt ttaaatcacg tttgccaaga
     3841 ccaatgcgaa ttagatcact ggatgatgca gcgactacag aagagacatt gctcactctt
     3901 gcgttgactc ccttagtgaa agagttgaag gctctggagt gtaataaaga gtcacaaata
     3961 tcaaaagtta aagcagcttt ggttaaagct gtaaatgaga tcgctgaacc tcatcaagaa
     4021 cgattttctt ctatttctcg acaaattcag acagggtttc aacatgtctt tcctgctctt
     4081 ggtgttcagc tcagtgttga aatgaatcca cctgagctta aaatagacaa tttattaaag
     4141 caggggtctg gtttattagt caaagaggct gcgggaaact ctcgcattgg gcaacagggg
     4201 acaggagcaa gacgagctct tttttgggca atgctccagg ttcataatga aattagccga
     4261 caaaatgaaa agcgagaagc tcttcttaaa tcctttagag aacaacttaa aaaagaggtg
     4321 aggaaaaatg taaaaagtga aaatataaat cttctgaaac aacaaataga tgctattgaa
     4381 aatggcgccc ctgttcctga agatacagac gatccagcct tgcccggata tattctttta
     4441 atggatgagc cagaaaatgc gttacaccca atggctgcca gagcggcgca agcgcatctt
     4501 tatgaacttg gtaaacatcc agattggcag gtgctattaa caactcattc accatacttt
     4561 ataaaccctc ttgaggatca tactacgatt gctcgtatgc aacgttcaac agatgggaaa
     4621 tcactttctc cacgcttgta tgtggctgat gaagctaact tctcacctga tgagaaagat
     4681 aatttacagg cactacaact tacagatatt ggttttgctg aaatattttt tgggagttat
     4741 ccaattattg ttgaaggtga tactgaacat gcggcattta tttcagcaat cactaaagaa
     4801 aaacatgaga tgtcaggtaa ggtaagtatt gtcagagcca gaggtaaggc ggttctggtt
     4861 cctttgataa agatgttaaa tcattttaaa gcagattttg ggattgtaca tgatattgat
     4921 tggccttatc gtagagatgg ttcgaataat ggttcatgga cgcttaatac gatcattaga
     4981 aatgagatta taaaatgcag gaataatggg caaaaagtat atcatcgttg gagtgtcccc
     5041 gattttgagc gttttttagg tggtgaggag ttggggaaag ataaacccta tacagcattt
     5101 aatcgaattt cgcgcgatga aaaattaaaa gaaaagattc aaaatcttat aattaatttg
     5161 tttgaaggga agtgttatga tcctgatgac tttgaacccg atgatgattt taatgctcaa
     5221 ctcatggaac aattaaaaat atgggcaaaa aataatggtg aaagtgataa tgttcgcgtt
     5281 atgggatgct gattataaca ttggttaatg gctgggtttc atataaatga ctttgcataa
     5341 ctaattcgtt tttgctgttt ttatataaag cctttaggac gttgtggaag gggaagtgtt
     5401 gcaatttatt cagtgagata gtttgtttgt agggtattta aacgcatttt ggtgatcatt
     5461 tgttgttcag gatagtgtgg cgaatatgaa tggtaaatat aatgtcagga gtgaattgtt
     5521 agctcgttgt atcggaacag gtagattgaa aggagatgtg gtaagtgatt ttattgggtt
     5581 taatggtagc aaacagatag gttatgtttt attaacttta tttttaataa aagtaa
//
LOCUS       DAZFRG010000277         5631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFRG010000277 DAZFRG010000000
VERSION     DAZFRG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.279"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..706
                     /locus_tag="ACHKUC_004354"
     CDS             <1..706
                     /locus_tag="ACHKUC_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6620488.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="ACHKUC_004355"
     CDS             complement(1909..2100)
                     /locus_tag="ACHKUC_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO6620489.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="ACHKUC_004356"
     CDS             2165..2488
                     /locus_tag="ACHKUC_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO6620490.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2697..3128
                     /locus_tag="ACHKUC_004357"
                     /pseudo
     CDS             2697..3128
                     /locus_tag="ACHKUC_004357"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="ACHKUC_004358"
     CDS             3166..3696
                     /locus_tag="ACHKUC_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO6620491.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="ACHKUC_004359"
     CDS             3703..3936
                     /locus_tag="ACHKUC_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO6620492.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="ACHKUC_004360"
     CDS             4417..5469
                     /locus_tag="ACHKUC_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620493.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5483..>5631
                     /locus_tag="ACHKUC_004361"
     CDS             5483..>5631
                     /locus_tag="ACHKUC_004361"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO6620494.1"
                     /translation="MLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVILSWI
                     VLFIK"
BASE COUNT         1486 a         1154 c         1514 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAZFRG010000278         5545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFRG010000278 DAZFRG010000000
VERSION     DAZFRG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.280"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            162..1346
                     /locus_tag="ACHKUC_004362"
     CDS             162..1346
                     /locus_tag="ACHKUC_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO6620495.1"
                     /translation="MNDPAVMEARAELNSAQSRTEQARSAHWPVITATGSKLLSQSHR
                     YSYDYDSEDILPGIRGEVNIFASGAIEADVRRSESEAEYYHYKVKETGEERIRSFVSL
                     YLDALREKQSIEVLKQSLSRHNSILDDLNTISIHDTGRESEFVQADARRLMVRQQLNT
                     RNRVLKSTLGKLSTWTINPVTEADLENPFSLMTESRLLTDFTQAPQTGNPSWLASQAD
                     VESKKAALRAQELARYPRIDLTGSVTRDDRQIGVNLSWDLFNRNASYGVSEKAAQIAA
                     ATGRLDSVARLIDETGRLSLITVRESRVEMETLRRQEQASAKVVDFYRLQFRVARKTL
                     IELLNAENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSDAKNNGAEGQV
                     NK"
     gene            1343..3490
                     /locus_tag="ACHKUC_004363"
     CDS             1343..3490
                     /locus_tag="ACHKUC_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO6620496.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAEALAASTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRALEDIPSLAVPVLLLLNAQEAIVVVRIKGTGLTREYHVLQPGGLR
                     NKLSHEALMAVYSGYCWFVQPQATQDRRSDLPEYDMSGGWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHNDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNEI
                     VRRPVERERQTHYLTVKDIQGEFRFSDVSFSYKSGLPEALSGLSLSIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVALQQLDPSFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQIGEGGLGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGSRDEILKKLSGKQQNDENRPAAS"
     gene            3550..4797
                     /locus_tag="ACHKUC_004364"
     CDS             3550..4797
                     /locus_tag="ACHKUC_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO6620497.1"
                     /translation="MTEKMERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFIIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQID
                     EKQNKYLTEAGSELVKTETDLAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKKLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5072..>5545
                     /locus_tag="ACHKUC_004365"
     CDS             5072..>5545
                     /locus_tag="ACHKUC_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620498.1"
                     /translation="MKMIFTGKVSGEKTVLAAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDSTEVRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAGGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRTAE"
BASE COUNT         1465 a         1106 c         1509 g         1465 t
ORIGIN      
        1 attatcatgg tgatttctag cgcagttcat tctagtaatt actctaacta tctagagtgt
       61 taacaagaac ataagagatg aaaaatttat tgttctcgtt gttgttactt tcatgttcaa
      121 caattagtgc accgttttat tctttggtta gggaggccgt aatgaatgat ccagctgtta
      181 tggaggcccg agccgagtta aattctgcac aatcccgcac agagcaggct cgttctgcac
      241 attggccggt tatcactgct acgggaagta aactcctttc acagagtcac cgttattctt
      301 atgattatga ctctgaagat attttgcctg gtattcgtgg tgaagtgaat attttcgctt
      361 caggagcaat tgaggcggat gtacgtcgga gtgaatcaga agcagagtat tatcattata
      421 aagtgaaaga aacaggggaa gaaagaattc gttcctttgt atctctatat cttgatgcac
      481 tcagggaaaa acaatccatt gaggtactta aacagagtct ttcccgacat aactcgatac
      541 ttgatgatct taataccatc agtattcacg atacagggcg ggaatctgaa tttgtacagg
      601 cagatgcacg cagattaatg gtccggcagc agcttaatac tcggaacaga gtgcttaaaa
      661 gtacactggg aaaactttcg acttggacaa taaatccggt aaccgaagcc gatcttgaaa
      721 atcctttttc tctgatgaca gagtccaggt tgttaactga ttttacacag gcacctcaga
      781 caggaaatcc atcgtggctt gctagtcagg ctgatgttga gagtaaaaag gcggcgctga
      841 gagctcagga acttgcccgg tatccccgga tagatttaac gggctctgtc acccgagatg
      901 accggcagat aggtgtcaat ctgtcctggg acctttttaa ccgtaatgcc agttacggtg
      961 tcagtgaaaa agctgcgcag atagccgcag ctacgggccg actggactct gttgcccgac
     1021 tgattgatga aaccgggcga ttatctctga taacggtcag agaaagtcgg gtggaaatgg
     1081 aaacgcttag acgtcaggaa caggcctctg ccaaagttgt agacttttat cgtctccagt
     1141 ttcgtgtggc aagaaaaaca ctgattgaat tacttaatgc agaaaatgaa ctgtacagtg
     1201 tcgggctttc ccgggttcag acagaggatc agatgctcca cggaatgctg gattatctct
     1261 attcccaggg aatgctcctg aaatggagcg gagtgaatct ttctgatgca aaaaataacg
     1321 gggctgaagg gcaggtaaat aagtgaagag tatttttgaa ggattatcac aggtaacggc
     1381 attactgggt acacctgtat ctgctgaggc tttggctgcc agtactgtcc ggacagatgt
     1441 atcggggatt gattttcgtt ctgttggcga atttctccgt agcgaagggt ttgataatca
     1501 tctttccagg cgggctctgg aggatattcc ttcacttgcg gttccagtgc ttcttttact
     1561 gaatgctcag gaagcaattg ttgttgtacg tattaagggt acaggtctga ctcgggagta
     1621 tcatgtgctt cagcccggtg ggctccgtaa taaactgtct cacgaagcgt taatggctgt
     1681 ttattctggt tactgctggt ttgttcagcc acaggcaaca caggatcgtc gttctgacct
     1741 gccggagtat gacatgagcg ggggatggtt ctggcgggtt ctgtggcgct tcaggccata
     1801 ttacggacag gttattcttg caagttttat cattaatttt cttgcactgg ttagctcctt
     1861 atatgtaatg aacgtttatg accgggtcat acctaatcga tcctgggaaa cgttatggat
     1921 gctgagtatc ggagtgattg cagccattct gtttgaatgt gctgcaaaat taattcggag
     1981 ctatctgacg gatattgcgg gtaagaaggc agacctgatt atcagctctg ccctgctacg
     2041 ccgggtgatg gcggtgaaaa tgtcggacag accagcatcc tccggttcat acgccagcaa
     2101 tttgcgggaa tatgagtctg ttagggagtt tatgaccagt gccacgttgc tggcttttgt
     2161 ggatttgcct tttatcctgc ttttcatcgg tgtcatcgga cttgtggggg gctggcttgc
     2221 tgttgttcct cttacactgg ttccactggt gattctggca ggaatattac tgcagcgacc
     2281 gctttcccgt catattaatg cctccatgcg ggaatcatcg cagcgccagg gattacttgt
     2341 ggaggctctg gacggaatag aaactctgaa gctgaataat gcggttaact gggtccagcg
     2401 tcgctgggat ggatatactg ccagtgtcgc ccgttcgtcc atccgggtaa aaaataccag
     2461 taatgtcatt atctgttttt ctacagcagt tcagcaactg aatactgttc tgctggtcat
     2521 tctgggaact tatcttattc ataatgatga cccacataac cgaataacca tgggggcact
     2581 gattgccgcg gttattcttt ccggtcgtgc gctggcacca cttggtcaga ttgcatcact
     2641 ggccacccgt tttcagcagg cccgcttggc catgcagggg gtgaatgaaa ttgtccgccg
     2701 tccggtggag cgggaacgtc agacacatta tctgacagtg aaggatatcc agggagagtt
     2761 tcgtttcagt gatgtctcct tttcttataa gtccggatta cccgaggcat tgtccggtct
     2821 gtcactcagt atccgacctg gagagcgggt gggtattatt ggctcaatag ggtgtggcaa
     2881 atcgacgcta ctgaaaatga tggtggggct gtaccgtcag aacaggggac aaattactct
     2941 tgacggtgtt gccctgcagc aactggatcc cagttttgtc cgagacaaag tatttcttct
     3001 tgaacagaat ccccggcttt ttctgggaac tctgcgtgaa aatctgcagc tttcgcgtct
     3061 accgggaagc ctgtcggatg tggatatgat taatgcagcc ggacgtttcg ggtttgaccg
     3121 ttttatccga catcatcctc gcggacttga tatgcagata ggggaaggcg gtctggggct
     3181 gtcaggagga cagaggcaga tggttgccct gacgagaatg gttctgggtt ctccccgggt
     3241 ggtactgctt gatgagccca cgacaggcct tgatctgcag actgagtctg ttgttctgaa
     3301 aggtctgagc cactggagtc ggggacgcac aatggtggtg gtgacacatc gtctgcaggt
     3361 tctgaatatt gttgaccgta ttgtcgtgat gaatgaaggg cgggtaatga tggacggttc
     3421 ccgtgatgaa atactgaaaa aactgtcagg gaaacagcag aatgatgaga acagaccggc
     3481 agcttcctga acctcaaccg gataagaaaa aagagcagat atgtttaaca gtttgtggac
     3541 cgcagggata tgacagaaaa aatggaacgg aataaagcgt cgtcagaagt ggtcaggcag
     3601 tcagacctgg cgctgttgac agatgtacag gcagcccttc agcaggaaaa acatcatgcc
     3661 cttttcctga tggtgatttt tctgctggta ctggtagtga cgtttattat ctgggcatgg
     3721 aacagccctc tggatgaagt gacccgtggt cagggaagta ttattcccgg tagtagggag
     3781 caggttatac agactcttga cccagggatt ctgaaaacac ttgaggtcag ggaaggcgat
     3841 attgttgaga aaggtcaggt actgctgaca ctggatgata cccgaagctc agccatgttg
     3901 agggaaagtg aagcccgggt gaataacctg gaggccgtca gggcaagact ccgtgcagaa
     3961 gcttattctg aatcactgac atttccggat aacgttcctg aggatttgcg ggagcgggaa
     4021 agtacggtct attttctccg gaagacggag ctggcgcaga gcatcgccgg ccttaagcag
     4081 agtaaggctc tgctggacag ggaaatagca atgacacgac caattgtacg tgaaggggcg
     4141 atgtcagaag tcgaactgtt acgtatgcag cgccagtcag ctgaactgca gttgcagata
     4201 gacgagaaac agaataaata cctgacggaa gccggttctg agctggtgaa gacagaaaca
     4261 gaccttgctc aggcaaaaga gaatatggct ggcagagccg acccggttga gcgttcacgt
     4321 ctcagggctc ctctgagagg tatcgtaaaa aatatacggg taaacacact aggaggggtg
     4381 gtttccgcag gacaggacat aatggaaatt attccgctgg aagatcagtt actgattgaa
     4441 gcttatataa atccacggga tgttgcctat gtcagaacag ggatgccagc acttgtaaaa
     4501 ttgactgctt atgattatgc catttatggt ggtcttgacg gagtggtaac actggtaagt
     4561 ccggatactt tacgtgatca gaaacgtccg ggagatttaa aactggaccc taatgaagct
     4621 tattatcggg tattggttac caccagtaat aattatctta cagacaggaa tggaaaaaaa
     4681 ttaccagtta ttcccggaat gatagccagc gtagatataa aaaccgggcg taaaacagta
     4741 ttccagtatc tgattaaacc gataaccaga atgaagcagg cacttcagga aagataataa
     4801 aaagcactta cagggaaggt gttttaaatg agcggaataa tttcattttt attctgccag
     4861 gatcattgca catgctgtat gtgatctgta gctaaagata acacaagatg tttgcctgtc
     4921 aggggaaaat aaaactgaaa aacgtaatga ttgaggaggc ttagaaagcg tcaaattaat
     4981 tatatgtaac tagattcgtt tttatttaat gactcccttt agcggataaa acacaagagc
     5041 atgctggttt tattaacctg aggatactta tatgaaaatg attttcactg gcaaggtcag
     5101 tggagaaaaa acagtactgg cagccggagc ccgtcataca gtaaaggcac aggccgggga
     5161 acagtatgga ctggttgatg aggtgacagg tctggttccg gatggggtgg aagctgaccg
     5221 ttcaggcgat gatttaattc tacgtaaaaa ggaagatagt acagaagtcc ggattgaagg
     5281 attctgggaa gaatgccagc cgggtgaaac acagtgtaca gcagtattta atgttgtggg
     5341 tgaaaatggc caggtaactg aagctgtcct gacccaggat ggccctgtac tggagaattt
     5401 tactgcaggg caaagtggca cattagctgg aggtatcaat aacgggatta tctggggggg
     5461 gattgctttt ggtgcagggc tggctgctat tgcactggct gccggaggcg gtggtggaag
     5521 cagtcgtcat aagcgtacag ctgaa
//
LOCUS       DAZFRG010000279         5538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFRG010000279 DAZFRG010000000
VERSION     DAZFRG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.281"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..1436
                     /gene="manD"
                     /locus_tag="ACHKUC_004366"
     CDS             222..1436
                     /gene="manD"
                     /locus_tag="ACHKUC_004366"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO6620499.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1503..2786
                     /locus_tag="ACHKUC_004367"
     CDS             1503..2786
                     /locus_tag="ACHKUC_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620500.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3038..3955
                     /gene="oxyR"
                     /locus_tag="ACHKUC_004368"
     CDS             3038..3955
                     /gene="oxyR"
                     /locus_tag="ACHKUC_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO6620501.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3938..5338)
                     /gene="sthA"
                     /locus_tag="ACHKUC_004369"
     CDS             complement(3938..5338)
                     /gene="sthA"
                     /locus_tag="ACHKUC_004369"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO6620502.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1240 a         1268 c         1504 g         1526 t
ORIGIN      
        1 catttttaat gattaatgga gtttgttatg tgattattta acgtgttgtt ttaacgatga
       61 tggctaatta ttcaggtggt ttttgagggt gttttttctc ctcggtagaa atttttattt
      121 ttaatgtgat cacggttgaa taatcacttt tgctctcttg tatggtagta ggcgtaaaat
      181 taagtttgaa gtgtctattt acaatacagg agattgcaat aatgaaaatt attgctgtgg
      241 atgtttttgt tacttgtccc gggcgtaatt tcgttacgct taaaataacc actgaaagcg
      301 gactttgtgg attaggagat gccacgttaa atggtcgcga attgtcggtc gcttcttatc
      361 tgaaagatca tctttgtcct caattaattg gccgcgatgc cagccgtatc gaagatatct
      421 ggcaattctt ttataaagga gcttactggc gacgtggtcc cgtcacgatg tcggccattt
      481 ctgccattga tatggcgttg tgggatatca aagctaaagc ggcaaatatg ccattgtatc
      541 aactgcttgg tggcgcttct cgtgaaggcg tgatggttta ttgccacacc accgggcgga
      601 cgattgatga agtccttgaa gactatgcaa agcaccagca gatgggcttt aaggcgattc
      661 gtgtgcagtg tggtgtaccg ggtatgcaaa ccacctatgg tctggcgaag ggcaaaggac
      721 tggcgtatga accagcaacc aaaggcctgt ggccagaaga gcagttgtgg tcttcagaaa
      781 agtatctcga ttttacgccg aagttatttg aagcggtgcg taataagttc ggttttaacg
      841 aacatctgtt gcatgacatg caccatcgcc tgacccccat cgaagcggca cgctttggga
      901 ggagcattga ggattaccgc ctgttctgga tggaagatcc gacgcctgcc gagaaccagg
      961 aatgttttcg tctgattcgc cagcataccg tgacgccgat tgcggtcggt gaagtgttta
     1021 acagtatctg ggactgcaag cagttaatcg aagagcagct gattgactat attcgcacga
     1081 cgattaccca tgcaggcggc attaccggga tgcgacggat tgccgatttt gcatcgcttt
     1141 atcaggtacg taccggttcg catggccctt cagatttatc gcctgtttgt catgcggcgg
     1201 cgctgcattt tgacctctgg gtgccaaact ttggcgttca ggagtacatg ggctattccg
     1261 aacaaatgct tgaagtcttc ccgcacagct ggcgcttcga tgaaggttat atgcacccag
     1321 gcgatgaacc ggggctgggt atttctttcg atgagaaact ggcggcgaaa tatccgtatg
     1381 acccggcgta tctccccgtc gcacgactgg aagatggcac attgtggaac tggtaatagc
     1441 ggttcgctta attattttct gaatattcat caaatcgatt aattccgctc aaggagctaa
     1501 aaatggacgt tgatgtgtca accagtgttg cgggtaataa accgcaacgg attcgtcgta
     1561 ttcagacagt gaccctggtt ttattattta tggcggggat cgttaatttt ctcgaccgtt
     1621 cgtcattgag cgtggcaggt gaagcaattc gtggtgagtt aggattatcc gctaccgaat
     1681 ttggtgtttt gctttctgca ttttctctgt cttatggttt ttcacaactt ccttccggta
     1741 ttttgctaga ccgctttggc ccacgaatag tgttaggcgc aggcttaata ttctggtcat
     1801 taatgcaggc attaacagga atggttaatt cttttagcca ctttatttta atgcgtatcg
     1861 gtctgggtat tggcgaagcg ccatttatgc ctgcgggagt aaagtcgatc accgactggt
     1921 atgcgcaaaa agaacgcggc acggcgctgg ggatttttaa ctcatctacc gttatcggtc
     1981 aggctatcgc gcctcctgct cttgtattga tgcaactggc atggggctgg cggacgatgt
     2041 ttgtcatcat cggcgtagca gggattctgg ttgggatctg ttggtacgcg tggtatcgca
     2101 accgggcgca gtttgtcctg accgaggaag aacggacata tctctccgcg ccggttaaac
     2161 cgcgtccaca gttacaattt agcgagtggc tggcgctgtt taagcatcgg acaacctggg
     2221 ggatgatttt aggtttctct ggcgtcaatt ataccggttg gctctacatc gcgtggttac
     2281 ccggttattt gcaggccgaa caaggtttca gtctggcgaa aaccggttgg gtggcggcaa
     2341 ttcctttcct cgcggcggcg gtcgggatgt gggttaacgg tattgttgtc gatcggctgg
     2401 cgaaaaaagg ctatgacctg gcgaagacgc gtaaaacggc gattgtctgc ggtttgatga
     2461 tgtcggcatt aggcacgttg ctggtcgtgc aatcttcctc gccagcccag gcggtagcgt
     2521 ttatctcaat ggcgctgttc tgcgtgcatt tcgcgggaac atctgcatgg gggctggtgc
     2581 aggtgatggt gtcagaaaca aaagtggctt ccatcgccgg gattcaaaac tttggcagtt
     2641 ttgtttttgc ttcctttgct ccgatcgtaa ccggttgggt cgtggatacc acacactcgt
     2701 ttaatctggc gctggttatc gcggcctgtg tgacgttcac cggggcgctg tgttacttct
     2761 ttattgtcaa agatcggatt gagtaaccgt tactatcagg ctctcttgct gtgggcctgt
     2821 agaataaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
     2881 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
     2941 tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcgttg ctatgctacc
     3001 tatcgccgcg aactatcgtg gcaatggagg atggataatg aatattcgtg atcttgagta
     3061 cctggtggca ttggccgaac accgccattt tcggcgtgcg gcagattcct gccacgttag
     3121 ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagttgggcg tgatgttgct
     3181 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
     3241 gcgtaccgta ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggcgagac
     3301 gatgtccggg ccgctgcata ttggtttgat tcccacggtt ggaccgtacc tgctaccgca
     3361 tattatcccg atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcaca
     3421 gacccaccag ttactggcgc aactggacag cggcaaactc gattgcgtga tcctcgcgct
     3481 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
     3541 tatctatgaa gaccacccgt gggcgaaccg cgaatgcgta ccgatggccg atctggcagg
     3601 ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg
     3661 ttttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg aaactctgcg
     3721 caacatggtg gcggcaggta gcgggatcac tttactgcct gcgctggctg tgccgccgga
     3781 acgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat
     3841 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
     3901 catccgcgca agaatggatg gccatttcga taaagtgtta aaacaggcgg tttaagccat
     3961 ttagcgcagc tacccgatag gcttccgcca tcgtcgggta gttaaaggtg gtgttgacga
     4021 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
     4081 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
     4141 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcacgtgcc agatgtttaa
     4201 actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt tcggttttgc
     4261 ctacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgtg
     4321 cgttggcttc acctttcacc agcgcctgcg cggcaatgcg cccctggtca taggctgccg
     4381 acgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
     4441 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
     4501 acgccagcga atctgtatta ccggtgcgac cgttggcata gagcagacag tcagctttca
     4561 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg atcttctcgt
     4621 actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag agagaatctg
     4681 acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta
     4741 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccgtaaataa
     4801 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcataa atgcgtggat
     4861 gggtgaaatc aacatctgtt ggatgatatg gacgagaacc gcaggcaata acaaattttt
     4921 cagcagttag tgtttcgacg ctgccgtccg ggcaatccag cgccaacgta tgctcatcaa
     4981 caaagcgagc gttcccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca
     5041 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
     5101 ggagcagtcg ggaatggtcg ctgtatagtg ggttttgatt gaattctata atgcggctga
     5161 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
     5221 tttgataacg ctcgataact gcgacgcgcg caccttgctt aaccaggccc atcgcagcgc
     5281 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
     5341 tagggcttac ctattcttat acataaaagc aacagaatgg taacatttta tcgcgggtaa
     5401 gccaattgat ccccgtcatt tatctggcta tatcgtgagc ggcctttgct ttgtcttcct
     5461 ccttcttatt ttatttattc actattaatc gctttttctt atggctgata gttttatctt
     5521 gccgaagcgt gctttttt
//
LOCUS       DAZFRG010000280         5514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFRG010000280 DAZFRG010000000
VERSION     DAZFRG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.282"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..741
                     /locus_tag="ACHKUC_004370"
     CDS             <1..741
                     /locus_tag="ACHKUC_004370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6620503.1"
                     /translation="KPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAK
                     GRALKQHVMAPLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALG
                     RRATGVELETERFEQTVREVQDLASQNG"
     gene            782..857
                     /locus_tag="ACHKUC_004371"
     tRNA            782..857
                     /locus_tag="ACHKUC_004371"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:815..817,aa:Ile,seq:cat)
     gene            867..943
                     /locus_tag="ACHKUC_004372"
     tRNA            867..943
                     /locus_tag="ACHKUC_004372"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:901..903,aa:Arg,seq:tcg)
     gene            958..1034
                     /locus_tag="ACHKUC_004373"
     tRNA            958..1034
                     /locus_tag="ACHKUC_004373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:992..994,aa:Arg,seq:tct)
     gene            1205..1636
                     /locus_tag="ACHKUC_004374"
     CDS             1205..1636
                     /locus_tag="ACHKUC_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO6620504.1"
                     /translation="MLGIFKKKTRRASAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPTLSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            1633..1797
                     /locus_tag="ACHKUC_004375"
     CDS             1633..1797
                     /locus_tag="ACHKUC_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6620505.1"
                     /translation="MTVKMRLAVVALLLFLVVMVDFSSRIMSVLADGVLVAGVVVVAF
                     PLLKKKASGD"
     gene            complement(1891..2022)
                     /locus_tag="ACHKUC_004376"
                     /pseudo
     CDS             complement(1891..2022)
                     /locus_tag="ACHKUC_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2763..4724
                     /locus_tag="ACHKUC_004377"
     CDS             2763..4724
                     /locus_tag="ACHKUC_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6620506.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGAVISTTSNPEYYFVVALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGASCNYNDIIPADHCLHDVQDLSKFSHPKASAAQYGCVG
                     QGLHIAKKLLPFIPANAGILLVPCCRGGSAFLAGDEGTFSESTGASETSARWGVDKPL
                     YKDLLTRTQAALKANPKNILLAVVWMQGEFDLKQGAYATQPGLFDSMVEKYRSDLSEF
                     GGQCLGGSPSSVPWICGDTTYYWKQTYSSQYDAVYGAYKTKSAKKIFFVPFMTDENGR
                     NVGTNEPSEDPDVADIGYYGAGGRTDAKTWTTADRKTHFGSWARRGIISDRLATAILV
                     HAGRNAEFITGKQPDTVKPTGPSGEGTEREPEAPVSNRTLMSLLASGEDLASQGWRYY
                     HKPASGDNVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGVELLKKGGR
                     VSVRFKLPGSLVPNQFALGIYWQLSSLPEGVTLAEEGNDMLMSFFLQTDATNLNAMHH
                     KKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKL
                     QLTGISKAATYTLLIDSVKVEVNNADIAA"
     gene            4860..5042
                     /locus_tag="ACHKUC_004378"
     CDS             4860..5042
                     /locus_tag="ACHKUC_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6620507.1"
                     /translation="MTFIHQVMLYFCTAVCVMYLLSGGYRAVRDFWRRQIDKRAAERI
                     SASQSAGSKPEDPLIP"
     gene            5080..5325
                     /locus_tag="ACHKUC_004379"
     CDS             5080..5325
                     /locus_tag="ACHKUC_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6620508.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            5402..>5514
                     /locus_tag="ACHKUC_004380"
     CDS             5402..>5514
                     /locus_tag="ACHKUC_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO6620509.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAA"
BASE COUNT         1315 a         1310 c         1641 g         1248 t
ORIGIN      
        1 aagccgtccg gacgctggaa cgggtgcaac aaggaaagcc tgcgggcgta tttccccgcc
       61 acagagcgca ttctgttcgc ggaacattat caggggccgt atcgtccgaa agatgccggg
      121 tatgaggcga agggcagggc actgaaacag catgtgatgg ccccgctgat tgcttacttt
      181 cgtgatgcgc gcgctgccct ggggataacg gcaaaacaga tagtggatgc cacaggaaag
      241 aaaaacatgg tgtcgcactg gttcagtgcc agtcagtggc agctgccgaa cgaaagtgat
      301 tatctgaaat tacaggcgct gtttgcccgg gtggcagaag agaagcatca gcggggtgaa
      361 ctggaaaagc cccaccacca gctgctggag acgtatactt cactgaaccg gcagtatgcg
      421 gaactgcaga gtgaatataa gcatctgcgg cggtattttg gcgtgacggc gcaggtgccg
      481 tacacggatg tgtggacgca taaaccggtg cagtactatc ccgggaaaca tccgtgcgaa
      541 aaaccggcag aaatgctgca gcagataatc agtgcgagca gtcgtccggg tgacctggtt
      601 gcagatttct tcatggggtc gggttcgaca gtcaaagccg cgatggcgct ggggcgtcgt
      661 gctacaggcg ttgagctgga gactgaacgt tttgaacaga cggtcaggga agttcaggat
      721 ttagccagtc agaacggatg atattgcagg attagttacg taccgttatt atcctgcgcc
      781 cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa
      841 tccagcaagg gccaccatat cacataccgc cattagctca tcgggataga gcgccagcct
      901 tcgaagctgg ttgcgcgggg ttcgagtcct cgatggcggt ccatttatcg gcattctgcg
      961 ttgttagctc agccggacag agcaattgcc ttctaagcaa tcggtcactg gttcgaatcc
     1021 agtacaacgc gccagactta tttttcccgg ctcgcttttg cgggcctttt ttgtatccgc
     1081 gccacgcccg gcgcatatca accacagagc ctttcggggg tgagcttacg gagtggtcag
     1141 tgtgactttc tctgtgggca gatcgctccc gggcgttggc tcacccaccc aaaggaacgt
     1201 cacaatgctt ggaatcttca aaaagaaaac ccgcagagcg tctgcggaaa ttaaaaagtt
     1261 tgaaaaacgc gatctggcac aggcagtgat taacgctgca tacctggtgg cttatgccga
     1321 tggtgaatgt gaggcttccg agaaagcgaa gatcgaacag gtattacgta accagcccac
     1381 cttgtccgcg tttacgtcag aaattaatgc gattagcgca accattatcg gtcagctgga
     1441 tacgaacttt aaaattggtc gtcgtgcggc gttacgtgag atcgaggatg tgaaacacga
     1501 tacgcgtgaa gcggaagacg tgctggatgt ggcggtggcc attgccgaag cagacggcga
     1561 aatagagccg gaagagcgca aggtgctgga agagattgcc ggtgttctgg gccttcgtct
     1621 ggagaatcat ctgtgacggt aaaaatgcgt ctggctgtgg ttgcgctcct gctgtttctg
     1681 gtggtgatgg tggacttcag cagccggata atgtcagtgc tggctgacgg tgtgctggtg
     1741 gcgggtgtgg tggttgttgc tttcccgttg ctgaaaaaga aagcatcagg cgattagcag
     1801 ggtatcagtt acccgttgaa atttttaaat acctcacaat tcaggcggtt gactgttgtc
     1861 tggtttgcgg ggagtttgtt aaaagaaact ggcatggtga atccccctgt gcggaggggc
     1921 aatcagcaac tggtgttttg tcaccgaccc ttatcctttc tgtgcgggtt caggtgctga
     1981 tactgaactc accgggaggc acccggcacc atgcaatggc acatagtgcc actctccagc
     2041 ccctctccgg aggggctttt ctgtgccgga tacatcacag tttctggaac cttaggtact
     2101 acagtatcag tcagggtgct atattttcag atgtgatgaa agcctgtcag caggcagggc
     2161 gtatcggaaa tgacccagta gagaaaacgt tgactcagat accggtgctg agttaccggg
     2221 aaaccggcat cacatgaccg ctatccttcc aggcccatcc gctccggtgg gcctttttac
     2281 tgcagaaaac aggttccccg ttaaatgcta tgttgctcac aattcagtaa gttgacagtt
     2341 gcctgtcaga ctgggcattt gttaaaaaaa tttcgcatgg tgaatccccc tgagcggagg
     2401 ggcgactggt gacggtataa tctctgatta tcaaaacgag aatgacgcgg gtttagtggc
     2461 accgggctga actcaccggg aggcacccgg caccatgtgc atgatgatac agatacgcgg
     2521 ctttagcccc tctccggagg ggctttcttg tgggcaaaaa aagcccgagt gggttcgggc
     2581 aacagcatga gatatacatt tttataatcg aatggatttt aaccagaatt cataaggctg
     2641 cgcaactgcg cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt
     2701 aacttccgga cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat
     2761 tcatggcatt taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac
     2821 gactgacaca aaaactgaag gaggggtggc agccatttgg cagtccggtg gccatcacgc
     2881 cttataccct gatgcaggcc attgcggcgg aaggtgatgt caccacacct gtggtggtga
     2941 agccgtcgga tggagaaggc gcagttatca gcaccaccag caacccggag tattactttg
     3001 ttgttgccct ggccgggcag tcaaacggta tggcgtatgg tgaagggctt ccgctgccgg
     3061 agacatatga ccgtccggac ccgcgtatta aacagctggc gcgtcgcagc actgtcacgc
     3121 cgggtggtgc gtcctgtaac tacaatgaca ttattcctgc ggaccactgc ctgcatgatg
     3181 ttcaggattt gagtaagttt tcacacccga aagccagcgc agctcagtat ggatgcgtgg
     3241 ggcagggatt acatatcgcg aagaaattgt tgccgtttat tccggcgaat gccggtattc
     3301 ttctggttcc gtgctgccgt ggtggttctg catttttggc gggcgatgaa ggtaccttca
     3361 gcgaatccac cggcgcaagc gagacctcgg cacgctgggg tgtagataag ccactgtaca
     3421 aggacctgct tacccgtact caggccgcac tgaaggccaa ccctaaaaat attctgcttg
     3481 cagtggtctg gatgcagggc gagtttgatt tgaaacaggg tgcatacgcc actcagccgg
     3541 ggctgtttga ttccatggtg gaaaaatatc gttctgacct gtcggaattc ggaggtcagt
     3601 gtctcggggg ctctccgtca tcggttccct ggatttgtgg cgacacgacc tactactgga
     3661 agcagactta ttcttcgcaa tacgatgcgg tgtatggtgc atacaagacg aaatccgcaa
     3721 aaaaaatctt ctttgtgccg tttatgacgg atgaaaacgg gcgaaatgtg ggtaccaacg
     3781 agccgtcaga agatccggat gttgcggata ttgggtatta cggagccggt ggtcgaacgg
     3841 acgccaaaac ctggacgacg gctgaccgta aaacgcattt tggatcatgg gcacgtcgtg
     3901 ggattatttc cgaccgtctg gcaacggcga ttcttgtgca tgccgggaga aacgctgaat
     3961 tcattaccgg aaaacagcct gatacggtga agcccaccgg accttccggt gaaggtacgg
     4021 agagagagcc ggaagccccg gtcagtaacc gaaccctgat gagtctgctg gcgtccggcg
     4081 aagacctggc atcacagggc tggcgctatt atcacaaacc ggcgagcgga gacaatgtta
     4141 acaaaaacat tgctgaagcg gtggtcagtg atgcgggggc tacgggaggt aaggccctgc
     4201 aactgaataa accggaaaac cacatctggt ttctggagca tgatgcagcc ggacagggag
     4261 tggagttact gaagaagggg ggacgtgtga gcgtacggtt taagttgccg ggttcactgg
     4321 tgccgaatca gtttgccctg ggcatttact ggcagttgtc gtccctgccg gagggagtga
     4381 cgctggcaga ggaaggcaac gacatgctga tgtccttctt cctgcagacg gatgcgacga
     4441 acctgaacgc gatgcaccac aagaagccga atgcgaagct ggatacgttc ggggtctttg
     4501 ataacggatg gcacacactg gcttttgagt ttgccggaaa caacagcatt caggtgacgc
     4561 cggtactgga tgagaaacgg gggacgccgt tcacactggt gaaatctccg gcatcagggg
     4621 cggcggacaa actgcaactg acaggcatat caaaggcggc gacatatacg ctgctgattg
     4681 acagtgtgaa ggtggaagtg aacaacgcgg acatcgcggc atgataaaaa aagccgccag
     4741 cggcaggaat ggacgctggc ggaggtaatt ccaatggaga atgtaaagaa aagatgcttt
     4801 cgtatatcgg ttttttaaat gaaaacagtt ctcattgtca accataacgg taagaaatta
     4861 tgacatttat tcatcaggtg atgctgtact tctgtacggc agtctgtgtt atgtatcttc
     4921 tttcgggtgg gtacagggca gtgcgcgatt tctggcgcag gcagattgat aaaagggccg
     4981 ctgagagaat cagcgccagt cagtcagccg gaagcaaacc cgaagatccg ctcattccgt
     5041 agtcactttc ttgacaacac ctttcaacga gaaaatccca tgtcagaaat cacatccctg
     5101 gtcactgctg aggcagtgaa ggaagtcctg cgctctgaag aagtccggag tgcactgaaa
     5161 cagaaactcc gccagaacct tgaggcgcgt cttgatgcag aagtggatgc cattctggat
     5221 gagctgctgg gcgtaccagc ggttccggag ccggaaggta tcgcgggtga cgggagtgct
     5281 tcagatggcg gtgaacccac acctgacagc gacatgatga tgtaagcctg cgcaagggac
     5341 catcggtgtg tgccggtggt ctttatattg ttgtgagctt ccggataacg ggagacgggg
     5401 tatgtaccag atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtgggaac
     5461 gggctactgg ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggc
//
LOCUS       DAZFRG010000281         5495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFRG010000281 DAZFRG010000000
VERSION     DAZFRG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.283"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..172
                     /locus_tag="ACHKUC_004381"
     CDS             <1..172
                     /locus_tag="ACHKUC_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620510.1"
                     /translation="TIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHDMNAGFDEK
                     ILFPSSVESTAG"
     gene            183..350
                     /locus_tag="ACHKUC_004382"
     CDS             183..350
                     /locus_tag="ACHKUC_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620511.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            347..691
                     /locus_tag="ACHKUC_004383"
     CDS             347..691
                     /locus_tag="ACHKUC_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620512.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            983..1138
                     /gene="hokD"
                     /locus_tag="ACHKUC_004384"
     CDS             983..1138
                     /gene="hokD"
                     /locus_tag="ACHKUC_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6620513.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1304..1954
                     /locus_tag="ACHKUC_004385"
     CDS             1304..1954
                     /locus_tag="ACHKUC_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620514.1"
                     /translation="MNENQLAPCWEFQPYLAENYVRHLLAEIANVLEQLYYHKHALDS
                     NWSEGVRAYDWVRNHLIQNEDAIPGLEMISKGLDYVVALNKVPLQFTKDCINNPKKKH
                     RLRRNKVEYEQLSLFGDVEAEQDITWRVIAEPFLSEEGDGELESTLPRWEVALVGFNT
                     YGAQISIVSHQSTASMPLMPLDYNTLPDEAEINKVPLRRRTKDKDLDVSSDGTSGE"
     gene            1935..3038
                     /locus_tag="ACHKUC_004386"
     CDS             1935..3038
                     /locus_tag="ACHKUC_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620515.1"
                     /translation="MEHQVNNFTEYRGDKLKLARMAVGLSCEELAEKIGKTKQFVSKL
                     EKGCRPSEQCLELISSALMIKSSFLFTERKYALESDVCHFRSKKSRTQTLTNSVLARA
                     EILNIIISAVEGEIEFPDVNIPEHPGAELLTPNDIERVAEDCRRAWNLGLGPISSMVK
                     LAESLGVIVAHVTGVDDRVDAFTVHNNRPVIIRNNVKKSICRFRSDLGHELGHLVMHE
                     GITTGDKLTESQADHFSSALLVPRLSFIKEFPRIRGKQFDWNALVEFKLRWKISLKMC
                     IYRASALGLLTQEQARTGYMHLNSRGYTRVEPGDELLRPEEPGMLAEAIEMLDDATWL
                     RILMKTGLSQDLIRELFSINRPITNPRNIFQIV"
     gene            3196..3270
                     /locus_tag="ACHKUC_004387"
                     /pseudo
     CDS             3196..3270
                     /locus_tag="ACHKUC_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3429..3701
                     /locus_tag="ACHKUC_004388"
     CDS             3429..3701
                     /locus_tag="ACHKUC_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620516.1"
                     /translation="MAHNFKFRNSDSAFASRHGWRSHAFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFRKALTLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            3703..4749
                     /locus_tag="ACHKUC_004389"
     CDS             3703..4749
                     /locus_tag="ACHKUC_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6620517.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEETVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            4762..5136
                     /locus_tag="ACHKUC_004390"
     CDS             4762..5136
                     /locus_tag="ACHKUC_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6620518.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            5133..>5495
                     /locus_tag="ACHKUC_004391"
     CDS             5133..>5495
                     /locus_tag="ACHKUC_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6620519.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCY"
BASE COUNT         1485 a         1177 c         1449 g         1384 t
ORIGIN      
        1 caccattggc gataaaagca tttacctgta tgacctgcgc cgcatggatg aaatcatgga
       61 ggctcttgat aatcgttcgt caatggattg gtgtgttgct gtccatgata tgaatgcagg
      121 gtttgatgaa aagattttgt tcccctcatc agttgaaagc actgcgggtt aaggagtaac
      181 acatgaccac tattaccaaa gaacgtattg aattgttcat taaaaacccg cttgaaaacg
      241 ggcttacccg tggtgaacaa atggaactgg cacggattgc gctggcatcg ctggaagcag
      301 agccggttgt gttctggttt gaaaaatatc aagaaggggc tacggcatga cgacttttac
      361 cagagagcag ttaatagctc acgcagagga gactattgaa gcacagagac tgtgcatacc
      421 gggtacaatc gaccatgaca tcatccgcac atataagatg gatattgctg ttctggaaat
      481 cgcactggta tcgctggcag cagagccagc cggtaaattg catgaataca aaccagtggg
      541 atatcagcgt ctggtcgatg agttaaccat gctggtaaag cagttaacct ggcaactgag
      601 gaaagcgaag ccagactgca aattaccgga taaggcgatg agttatctgg agcggaacgg
      661 actgataagc gtggaggata ttttacgatg acctggcctg aagcattaac aacggtagga
      721 attgcgatgg cggtgacgct gatggtgtat tcgatttgcc gctggggata aaaacggttt
      781 gcgggaaaag gagagttaag tagaattgca gcgggtgctt gaggctatct gtctcaggca
      841 tgaacaccaa aaggcagata gagaaaagcc ccagttaaca ttacgcgtcc ggcaagacgc
      901 ttaacattaa tctgaggcca tatctatgct ctacacacgt aggttagcct cttacgtgcc
      961 gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgctaatcg ccctgatcgt
     1021 catctgttta accgtcattg tgacggcact ggtaacgagg aaagacctct gcgaggtacg
     1081 aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg aggagtaaga
     1141 gacccggcgg gggagtaatc tcccgccacc tctgatgtgc caggcatcct caacgcaccc
     1201 gcacttaacc cgcttcggcg ggtttttgca ttagtctggt tgacaaaaat agaaaaatgc
     1261 gaaaatatgt ggtttacgaa ttctaaaaaa agcgaaactt gaaatgaatg aaaatcagtt
     1321 agctccttgt tgggaatttc aaccttatct tgctgaaaac tatgttcgcc acttgttggc
     1381 ggagatcgct aacgtacttg agcagctgta ctatcataag cacgcattag acagcaactg
     1441 gtctgaaggt gtaagggctt atgattgggt cagaaatcat cttattcaaa atgaagatgc
     1501 aattcctggc cttgagatga tttccaaggg gttggactat gtagttgctt taaataaagt
     1561 tccgctacaa tttaccaaag attgcattaa taaccccaaa aagaaacatc gtctgcgtcg
     1621 aaataaagta gagtatgagc agctctcatt gtttggtgat gttgaggctg agcaagatat
     1681 tacatggcga gtcatagctg agcctttttt atccgaagag ggcgatggtg aattagagtc
     1741 cacactgcct cgttgggagg tagctcttgt tggatttaat acttatggtg ctcagattag
     1801 tatagtttct catcaatcta cagcatcgat gccgcttatg cctcttgatt ataacacact
     1861 ccctgacgaa gcggagatta ataaggtgcc tcttcgtcgg cgtacgaagg ataaagattt
     1921 ggatgtgagc agtgatggaa catcaggtga ataacttcac tgagtatcgg ggtgataagc
     1981 tcaaactagc gagaatggct gttgggcttt cttgtgaaga gttagccgaa aaaattggca
     2041 agacaaagca attcgttagc aaactggaga aggggtgcag gccatcggag caatgtcttg
     2101 agttaatatc ttcagcgctt atgattaagt ccagttttct ttttactgaa cgaaaatacg
     2161 ctctggaaag tgatgtctgc cattttcgga gtaagaagtc caggactcaa acgctgacta
     2221 atagtgtatt ggccagggct gagattctta atattataat ttctgctgtt gaaggtgaaa
     2281 tcgaatttcc tgacgttaac ataccggagc acccaggggc tgaattactt actccgaatg
     2341 atattgagcg agtggcagaa gattgtcgcc gtgcctggaa tttaggtctt ggccctatat
     2401 catcaatggt taaattggcg gagagtttag gggtaatcgt tgcgcatgtt acgggagtcg
     2461 atgatcgtgt tgatgctttt actgttcaca ataacaggcc tgttatcatc aggaacaatg
     2521 ttaaaaaaag catatgtaga tttcgctctg atttaggtca tgaattaggg catttagtaa
     2581 tgcatgaggg cataacgaca ggtgataaac ttacggaatc acaagccgat cacttttcga
     2641 gcgccttatt agttcccagg ttatctttca ttaaagaatt tccacgaata cgaggtaagc
     2701 aattcgactg gaatgctctg gttgaattta aacttagatg gaaaatcagc cttaaaatgt
     2761 gtatttatcg agccagcgca ttaggcttat tgacccagga acaggcaaga actggctata
     2821 tgcatcttaa ttccagaggg tatacgagag ttgaacctgg tgatgaactt ttgcgccctg
     2881 aagaacccgg catgctggcc gaggcgattg aaatgctgga tgatgcaacc tggctaagaa
     2941 ttcttatgaa aactggcttg agccaagatt taattcgtga gttgttctcc atcaacagac
     3001 ctattacaaa tccaagaaat attttccaga ttgtttgagt atacccgctt cggcgggttt
     3061 tttgttttta ttttcaacgt gtttgaagtt ttggacggtg ccagaataga atcaaaaata
     3121 cttaagtagc gcgcagggag aagagggatg gaccccgaac agggggagtg ctatttatct
     3181 ggaaggattc tgttgatgaa aatcgaagaa ttacgtgaaa tttttagtga agatggcctc
     3241 tatgctgtgc gcgttgagaa tgggggtatt acctacacag cgttaattcc tgatgatcat
     3301 gtagtgttat ctgttgaggc attcattgaa tacttggaaa gactcggttt caaggtagtt
     3361 cgggaataag ttataatacg tgagccagcc tgaacaactg gcaacctgca gcgccattgg
     3421 agatgacaat ggcgcataat ttcaaatttc gcaattctga ttctgccttt gccagcaggc
     3481 acgggtggcg ttctcacgca ttcaaatatg actggtatca gcacgatccc tgtactgaag
     3541 aacaggccga atggctgatt cataactacc gcagacgtgg atatgagttt aggaaagccc
     3601 tcaccctcga ttatcgtcac tggataatct acgtcagact cccttattcc gaacgcccac
     3661 cgcgtccgtc ccgcacattc cagcaacgca tctggaggta acgtgcgggt attacttcga
     3721 cctgttctgg taccggaact cgggctggtg atcgttaagc cgggccgtga atccatgccg
     3781 gtattccaca atacccgggt actggtggag ccggaaccga aaagcatgcg taatctgccg
     3841 tccggggtcg ttcctgccgt tcgccagccg ctggcggagg ataaatcatt actgccattt
     3901 ttcagcgacg aacgagtgat tcgtgctgct ggtggcgctg gcgcattgtc tgattggtta
     3961 ctgcgccatg ttaaatcctg ccagtggcca cacggcgatt atcaccacag tgaaaccgtc
     4021 attcaccgtt atggtaccgg cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgc
     4081 gaccagacct ccgaatcact cgggcaactt gctcaccaaa acctgtctgc atggatgatt
     4141 gacgtcatac gccatgcaat gaatggctcg caggaacggg aattatcgct ggctgaatta
     4201 tcctggtggg cggtccgcaa tcaggtggcg gacgcgctac cggaagcggt attacgtcgt
     4261 tcgctggggt tgcgtgcgga aaaaatccgc tcaatgtacc gtgaaagcga catcgtaccg
     4321 ggagagcaga ccgccaccag catactgaag cagcgcacaa aaaatcttgc gccgctgcct
     4381 cacgcccacc agcaaaaccc gccacaggaa gagacggtgg tcagcattgc cgttgatcct
     4441 gagtctccgg aatctttcat gaaacgacct aaacgtcgcc gctgggttaa cgagaaatac
     4501 acacgctggg tgaagacaca gccgtgtgcg tgttgtggta agccagccga cgatccccat
     4561 cacctgattg gtcatggtca gggcggaatg gggacaaaat ctcacgatat tttcacgcta
     4621 ccgctgtgtc gggagcatca caacgagctt catgcggatc ctctggcgtt cgaagaaaag
     4681 catggttctc aggttgattt aatttttcgt tttcttgatc acgcctttgc aactggcgtg
     4741 cttgggtaaa agaggtgact gatgctcata gatttggttt taccttaccc gccgacggtg
     4801 aacacttact ggcgacgccg tggcagcaca tattttatct cggaggaggg aaagcgttat
     4861 cgccgggctg tggcgcttat tgttcgccag cagcggctga aattaagcct gtccggaagg
     4921 ctggcgataa aggtgattgc agagccaccg gataagcgtc gtcgcgacct ggacaatatc
     4981 ctgaaagcac cgctggatgc gctgacgcat gcgggagtgt taatggacga tgagcagttt
     5041 gatgaaatca atatcgttcg tggtcagcca gtatctggag gacgtctggg ggtgaagatt
     5101 taccccataa tgcatgaaga gcaggtcaaa aaatgaaact ggaagattta ccgaaatact
     5161 actccccaaa atccccttgc ctgaccgatg catcggcctc aacgtcaaaa gatgcgctga
     5221 gtatcactga tgtgatggcc gcgcagggca tgacacagaa tcgggctgag atggggtttt
     5281 ctgcgttcct ggggaaaatg ggcatcagta tgaatgacag ggcgcgggca acagaattac
     5341 tggcagatta tgcactcagt cggtgcgatc gtgtggcggc gttgagaaaa cttccggcag
     5401 aaataaaacc ggtagtgatg cgcattatgg cttcgtacgc ttttgaggat tatgcccgca
     5461 gcgcagcgag taaaaagcag tgcccttgtt gctat
//
LOCUS       DAZFRG010000282         5470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFRG010000282 DAZFRG010000000
VERSION     DAZFRG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.284"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..491
                     /gene="eutS"
                     /locus_tag="ACHKUC_004392"
     CDS             156..491
                     /gene="eutS"
                     /locus_tag="ACHKUC_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO6620520.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            504..983
                     /gene="eutP"
                     /locus_tag="ACHKUC_004393"
     CDS             504..983
                     /gene="eutP"
                     /locus_tag="ACHKUC_004393"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO6620521.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQTVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            958..1659
                     /gene="eutQ"
                     /locus_tag="ACHKUC_004394"
     CDS             958..1659
                     /gene="eutQ"
                     /locus_tag="ACHKUC_004394"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO6620522.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1656..2459
                     /gene="eutT"
                     /locus_tag="ACHKUC_004395"
     CDS             1656..2459
                     /gene="eutT"
                     /locus_tag="ACHKUC_004395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO6620523.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2456..3472
                     /gene="pta"
                     /locus_tag="ACHKUC_004396"
     CDS             2456..3472
                     /gene="pta"
                     /locus_tag="ACHKUC_004396"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6620524.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     KQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNHESSLQ
                     TLVE"
     gene            3511..3804
                     /gene="eutM"
                     /locus_tag="ACHKUC_004397"
     CDS             3511..3804
                     /gene="eutM"
                     /locus_tag="ACHKUC_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO6620525.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3911..4198
                     /gene="eutN"
                     /locus_tag="ACHKUC_004398"
     CDS             3911..4198
                     /gene="eutN"
                     /locus_tag="ACHKUC_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO6620526.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4210..>5470
                     /locus_tag="ACHKUC_004399"
     CDS             4210..>5470
                     /locus_tag="ACHKUC_004399"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO6620527.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDECGKGTVSRDWVGRDAAKIA
                     AAIDLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNG"
BASE COUNT         1296 a         1429 c         1594 g         1151 t
ORIGIN      
        1 attcatgcgt tgcatatgaa agtttatgca ccacagcaaa tatctctcca ttcttagtga
       61 tctacctcac cttttaaacg cgcttgccga attttgttat ttactctgac gaaaaattgt
      121 cacgatacac gaaagttttt cacaggcggc gactcatgga taaagaacgc atcattcagg
      181 aatttgtgcc cggtaaacag gtcacactgg cgcatctcat cgcgcatccc ggcgaggaac
      241 tggcgaagaa aatcggcgtt ccggatgcgg gcgcaatcgg cattatgacg ctaactcccg
      301 gagaaacggc gatgatcgcc ggtgatttag cgttgaaagc cgccgatgtg catatcggtt
      361 ttctcgaccg ttttagcggc gcgctggtga tttatggctc ggtgggcgca gtagaagaag
      421 cgttatcaca aaccgtcagt ggtttaggac gcttattaaa ttatacgctt tgtgaaatga
      481 ccaaaagtta atacgaggca ttcatgaaac gaattgcgtt tgtgggtacc gtcggggcag
      541 gaaaaacaac gttatttaat gcgttgcagg ggaattatac cctcgcaaga aaaacacaga
      601 ccgtggaatt taatgataat ggcgatattg atacaccggg tgaatatttt agccatcccc
      661 gctggtatca cgccttaatt accacgctac aggatgtgga catgctgatc tatgtccacg
      721 gcgcgaatga cccggaaagt cggttgcctg ccgggttgct ggatattggc gtcagcaaac
      781 ggcaaatcgc cgtcatcagt aaaacggaca tgccagatgc tgacgtcgcc gcaacgcgaa
      841 agttattgct tgagacgggc ttcgaagagc cgattttcga gctaaacagc cacgacccgc
      901 aaagcgtgca gcaactggtg gattatctgg catctctcac caaacaggag gaagcaggtg
      961 aaaaaactca tcacagcgaa tgatattcgt gaagcacacg cgcgcggcga actggcaatg
     1021 tccgtggttc tgcgcgccag cattattacc ccggaagctc gtgaagtggc tgatctgttg
     1081 ggttttacca tcaccgaatg tgatgaatcg atcccggtaa cggcgtctgt tcccgccagc
     1141 gcatcagccg ataaaaccga aagccagcgc atccgtgaaa ccattatcgc ccaactgccg
     1201 gaaggtcagt ttaccgaaag cctggtcgcg cagctgatgg aaaaagtgat gaaggaaaaa
     1261 cagtcgctgg aacagggtgc actgcagccg agctttaaat cggtgaccgg caaaggcggc
     1321 atcaaagtta ttgatggcag cagcgtcaag tttggtcgtt tcgacggcgc acagccacac
     1381 tgcgtaggct tgactgatct cgtgaccgga gacgacggca gcagtatggc cgctggtttt
     1441 atgcagtggg agaacgcatt cttcccgtgg accctgaact acgacgaaat cgacatggtg
     1501 ctggaagggg agctgcatgt tcgccacgaa ggcgaaacta tgatcgccaa agcgggcgat
     1561 gtgatgttta tcccgaaagg ctccagtatc gaatttggta cgacatccag cgtgaaattc
     1621 ctgtatgtcg cctggccggc taactggcaa tccctatgaa agatttcatc accgaagcat
     1681 ggctaagagc gaaccatacg ctcagcgaag gcgcagagat ccatctccct gcggacagtc
     1741 gcctgacgcc ctctgcccgg gagttactgg aaagccgcca tctgcgcatc aagtttattg
     1801 acgagcaggg ccgcctgttt gttgacgatg aacagcagca gccgcagccc gttcacgggc
     1861 tgaccagtag cgatgaacat ccgcaggcgt gctgcgaact gtgccgccag ccggtagcaa
     1921 aaaagccgga tacgctgact cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc
     1981 gtctgggttt tcgcgctgtt ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg
     2041 aactggcgga accgtggcag ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta
     2101 tgcgtgccga tgcgctgggg gaaccgttgg gctgtcaggc gattgtcggg ctttctgacg
     2161 aagatctgca tcggctttct caccagccgc tgcgttatct cgaccacgat catctggtgc
     2221 cggaagccag ccacgggcgc gatgcggcgt tgctgaatct gttacgcacc aaagtgcggg
     2281 aaaccgaaac cgtagcggcg caggtcttta tcacccgcag ctttgaagta ttacgcccgg
     2341 acattttgca ggcgctgaac cgcctttcca gcacggtcta cgtgatgatg atcctgagcg
     2401 taacgaagca gccgctaacg gtgaagcaga tccaacaacg actgggggaa acgcaatgat
     2461 tattaaacgt tgtcgtgaac tggcgttgcg agcgcccgcc agagtagttt ttccggatgc
     2521 gttagatcaa cgtgtgctga aagctgcgca atatttacat caacaaggtc tggcaacgcc
     2581 cattctggtc gccaatccgt ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat
     2641 ggacgggcta caggtgatag atccgcatgg caacctcgca atgcgggaag aatttgctca
     2701 tcgctggctg gcccgcgcgg gcgaaaaaac gccgccggat gcgctggaaa aacttaccga
     2761 tccgctgatg ttcgccgccg caatggtcag cgccggtaaa gcggatgtct gtatcgcggg
     2821 caacctctct tccacggcga atgtgctgcg tgccggatta cgcattatcg gcttgcagcc
     2881 aggctgtaaa acgctctcat ccattttcct gatgctgcca cagtacagcg gtccggcgtt
     2941 gggctttgcc gattgcagcg tggtgccaca gccgacggcg gcacaactgg cggatatcgc
     3001 gcttgccagc gccgaaacct ggcgcgccat caccggagaa gaaccgcgcg tggcgatgct
     3061 gtcgttttcc agcaacggta gtgcccgtca cccctgcgtt gccaacgtca agcaggcgac
     3121 agaaatcgtc cgtgagcgcg caccaaagct ggttgtcgat ggcgagttgc agtttgacgc
     3181 cgccttcgtg ccggaagtgg cggcgcaaaa agcgcctgcc agcccgttac agggcaaagc
     3241 taatgtgatg gtttttccgt cgctggaggc aggaaatatt ggctacaaaa tcgcacaacg
     3301 acttggcgga tatcgtgccg tcgggccatt gatacaagga cttgccgcgc cgatgcacga
     3361 tctctctcgt ggttgtagcg tgcaggaaat tatcgaactg gcgctggtgg cagctgtgcc
     3421 gcgtcagaca gaagtgaacc acgaaagcag tttacaaaca ctggttgaat gaacggtccc
     3481 gttctggacc ctgataaaga ggaaagcaca atggaagcat taggaatgat cgaaacccgg
     3541 ggcctggttg cactgattga ggcttctgac gcgatggtta aagctgcgcg cgtcaagctg
     3601 gttggtgtta agcagattgg cggcggcctg tgtactgcca tggttcgtgg tgatgttgca
     3661 gcctgtaaag ccgcgaccga tgcaggtgcc gcagcggcac agcgtatcgg cgagctggta
     3721 tctgtacacg ttatcccgcg tccgcacggc gacctggaag aagtcttccc gatcggcctg
     3781 aaaggcgata gcagcaacct gtaatcacct tgtgtgacgt cgattcgggc gtcacgctct
     3841 ctcctttttc attttacctt ctgcggaggg tagggggagc ttttacctga aatacgccac
     3901 ggaggcgggt atgaaactgg cagtcgtcac tggacaaatt gtttgtaccg tacgccatca
     3961 cggactggcg catgacaaat tgctgatggt ggaaatgatt gatccacaag gtaatcctga
     4021 cgggcaatgc gccgtcgcca tcgacaatat tggcgcggga accggggagt gggtgttact
     4081 ggtgagtggc agttccgccc gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg
     4141 cgtgattggc attgtcgatg aggtggtgtc tggcggtcag gtcattttcc acaaataagg
     4201 cagaacatca tgaatcaaca ggatattgaa caggtggtga aagcggtact gctgaaaatg
     4261 caaagcagtg acacgccgcc cgccgccgtt catgagatgg gcgttttcgc gtccctggat
     4321 gacgccgttg cggcagccaa agtcgcccag caagggttaa aaagcgtggc aatgcgccag
     4381 ttagccattg ctgccattcg tgaagcaggc gaaaaacacg ccagagattt agcggaactt
     4441 gccgtcagtg aaaccggcat ggggcgcgtt gaagataaat ttgccaaaaa cgtcgctcag
     4501 gcgcgcggca caccaggcgt tgagtgcctc tctccgcaag tgctgactgg cgacaacggc
     4561 ctgaccctaa ttgaaaacgc accctggggc gtggtggctt cggtgacgcc ttccactaac
     4621 ccggcggcaa ccgtaattaa caacgccatc agcctgattg ccgcgggcaa cagcgtcatt
     4681 tttgccccgc atccggcggc gaaaaaagtc tcccagcggg cgattacgct gctcaaccag
     4741 gcgattgttg ccgcaggcgg gccggaaaac ttactggtta ccgtagcaaa tccggatatc
     4801 gaaaccgcgc aacgcttgtt caagtttccg ggtatcggcc tgctggtggt gaccggcggc
     4861 gaagcggtag tggaagcggc gcgtaaacac accaataaac gtctgattgc cgcaggcgct
     4921 ggcaacccgc cggtagtggt ggatgaaacc gccgacctcg cccgtgctgc tcagtccatc
     4981 gtcaaaggcg cgtctttcga taacaacatc atttgtgccg acgaaaaggt actgattgtt
     5041 gtggatagcg tagccgatga actgatgcgt ctgatggaag gccagcatgc ggtgaaactg
     5101 accgcagagc aggcgcagca actgcaatcg gtgttgctga aaaatatcga cgagtgcgga
     5161 aaaggcaccg tcagccgtga ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc
     5221 gaccttaaag ttccggaaca aacgcgactg ctgtttgtag aaaccaccgc agaacatccg
     5281 tttgccgtga ccgaactgat gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg
     5341 gatgccattg cgctggcggt gaaactggaa ggcggttgcc accacacggc ggcaatgcac
     5401 tcgcgcaata tcgaaaacat gaaccagatg gcgaacgcca ttgataccag cattttcgtt
     5461 aagaacggac
//
LOCUS       DAZFRG010000283         5462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFRG010000283 DAZFRG010000000
VERSION     DAZFRG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.285"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..67)
                     /locus_tag="ACHKUC_004400"
     CDS             complement(<1..67)
                     /locus_tag="ACHKUC_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HFO6620528.1"
                     /translation="MMKEKRNDAELKNRKTKRDYDY"
     gene            complement(45..392)
                     /gene="trbJ"
                     /locus_tag="ACHKUC_004401"
     CDS             complement(45..392)
                     /gene="trbJ"
                     /locus_tag="ACHKUC_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO6620529.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(322..867)
                     /gene="trbB"
                     /locus_tag="ACHKUC_004402"
     CDS             complement(322..867)
                     /gene="trbB"
                     /locus_tag="ACHKUC_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO6620530.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(854..1138)
                     /gene="traQ"
                     /locus_tag="ACHKUC_004403"
     CDS             complement(854..1138)
                     /gene="traQ"
                     /locus_tag="ACHKUC_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO6620531.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(1257..1601)
                     /gene="trbA"
                     /locus_tag="ACHKUC_004404"
     CDS             complement(1257..1601)
                     /gene="trbA"
                     /locus_tag="ACHKUC_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO6620532.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(1615..2358)
                     /gene="traF"
                     /locus_tag="ACHKUC_004405"
     CDS             complement(1615..2358)
                     /gene="traF"
                     /locus_tag="ACHKUC_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO6620533.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2351..2608)
                     /locus_tag="ACHKUC_004406"
     CDS             complement(2351..2608)
                     /locus_tag="ACHKUC_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO6620534.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(2635..4485)
                     /gene="traN"
                     /locus_tag="ACHKUC_004407"
     CDS             complement(2635..4485)
                     /gene="traN"
                     /locus_tag="ACHKUC_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO6620535.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(4482..5120)
                     /gene="trbC"
                     /locus_tag="ACHKUC_004408"
     CDS             complement(4482..5120)
                     /gene="trbC"
                     /locus_tag="ACHKUC_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO6620536.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVATTGDCRQVAHDLLAGKGDSGK"
     gene            complement(5129..5434)
                     /locus_tag="ACHKUC_004409"
     CDS             complement(5129..5434)
                     /locus_tag="ACHKUC_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620537.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
BASE COUNT         1348 a         1514 c         1204 g         1396 t
ORIGIN      
        1 cataatcata atcccgttta gtttttctgt tttttagttc cgcatcattt cttttttctt
       61 tcattataag tttcctctga tatttccgat gattcattca gaccgttcat gcggctgtaa
      121 tatatttcag acaccgaaat ccatcccaca attgccccca caaaaaacac aacagaaaca
      181 gaaaaggtaa gacacagcga aacgacaatt tccacagggc tggcgggata aaaaagcccc
      241 tgacggataa aggggatatt caggctgacg attattcccg tcgcgatgcc ggatattccg
      301 gcaatgagta acaccacctg cttatttcgt acctttttcc tctccgtaca tctgcagaac
      361 ggtatccatc cgcgccataa aactggcggc atccgttgcc ccctgcaaaa gcggtaatgc
      421 ctcaagtgtg ttgacgttga ccaggaaggt agtcggtgtg gccaccggga tattcgggaa
      481 gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc ggaaaggcgg tatccccctg
      541 accatccagg gtgtagggaa aaacggaaaa gccgtactgc tgtgccagct gtttcagtac
      601 cgggtcaaac tggtgacagt aagggcaatg cccctgcata aacagcacca ccttccagtc
      661 ggccaggtta acctgcctgc cgttgctgag gcggaaccag cggggagccg gcttttctgt
      721 tctggctgat gtgtctgctg ccggttgtgc aggctgcacg gccataccct gcggattcca
      781 gagtcgttca atttcatccc gggtggaggc ctgcacacct gtggccatca tcaacaaaat
      841 gaccggcagt aatttagtga gagacatgtc cgccctcctg ctgcccttcc atcatggcct
      901 gttgcctggc ttccagtgcc tcacgctctt ccaggctgac acgtgcaatc catgcatcca
      961 gcacctggta tgcgccgaac agcacaagga tggccgcaat caactccgcc agaaaaaaag
     1021 ccatccaggg tttgctgtaa acaagacggg cgacgatatg aaaccagaca cccagggaaa
     1081 atacccacat tcccgtaata tcaagccggg gtaaagagaa tctgtattta cttatcatgt
     1141 taaactccgg ttattattta catgagagta ttcatgtctg taatattcgc gtttgtgtga
     1201 tattaatcag atcaacctca ttattttgta tggtttttat taccgtttta tatgacttag
     1261 cctgtcataa gcccccttat aaaaaaccac atcactccgg accatgctga tgacaacata
     1321 atacaaacaa acatccttct gaaactgtca aacttcgtga taaccggtat ggtactgact
     1381 gccattgaaa caaccggtac ggcacactga ttcataaccg caccacaaag aaacaagaaa
     1441 ttatctggca gcatacatac acttatgaca tttactacaa agaagacaaa cgagctcagc
     1501 agataaactc ctttattatt tggtcctgta aagaaataac caacaacccc cacgataaac
     1561 aggctggcac ctgtaaaaaa acacaaatcc cctgtatgca tatataacct ttggttaaaa
     1621 attcggttta aaatcttcag aaacgttcag gaactgtttt gccaggtcgt cctgtgaaat
     1681 aaagccgtat gataacgggc gaacactgcc ctgtttcggg tcaaccagca tcatggccgg
     1741 gaaatatttc acaccgaggc gctgcgcctg cccctggtca gtccgggaat ccggcaacag
     1801 cggattaatc acgccatcca cggaaacggg aataacactc agaccatagg tatccctgaa
     1861 gccattaatg acctgcgcca gttgcccgtc gatggggtcc tgcccccggt aaaagaacat
     1921 gatgccgtag tgttcagcca gtttcgcaat ggcctgtcgc tgctgcgcct ggtctgccgc
     1981 cagctggttc cgtaccgtgc cgttgtaatg gctgtactgc aggttatagt ccagttcggg
     2041 atgtgccagc atggcctttt tagcactcat ggtgaaaagc ccggcctgct gagtccagta
     2101 attctgcagc cggaaatatt taacaaaatt atccacgccg ggatacagaa tggcttcgta
     2161 cagcgcccgc tttgttgccg tctgcagcgc ggccagtttc tgcataatat ccggctcctg
     2221 acggggggct gcaggtacag gtttattttc tttttccttc ggatttattt tctcgttata
     2281 ccactgccag cctgcatctt tccctgacgc cggaaaaata aagcagcaga gtaacagtgg
     2341 cagtaatgct ttattcatct tcattccttt catcattttt tctttcatca tctttctgca
     2401 ggccagtcag ggcagaaaaa caaaaataca gaataaacag gagcacaccg gagtaaagga
     2461 taacgaaagc agcggcaagc atcccgtctg ccgtggtttc ttttaccgta tcccacgacc
     2521 agataattac cggggaaatg acaatcgctg taatcgaaag ccggataaga aaatcaacaa
     2581 gacgattaga agtgaatata actttcatca tttcccccgg ttattttcca taccttactg
     2641 tcctgcctgt ttcagctggt cagcaatctg ctctttcact ttctgcgtca gaacgccact
     2701 gtccgggatt ttctggttat tcatcaggtc ttcgtagaag ttagtgaaat ccagtctgtc
     2761 gaactgaatt ttctgcagct catcaaccgt aatcccccga cagtcaggat gcttcgcact
     2821 gccaaacccg atacgcaact gcccgttacg cccctgctgc tggactatct gcgccagctt
     2881 cgaatcaaac tggcagtagc tgcgcttttt ctgcagacag acacccagca ctttccggga
     2941 gcagaactcg cccacactca ccgtcagttt gttgtccttt gcctttccca gcgccttttc
     3001 ttcactgttg cacttcgcca gccccacatc ctgaccccag ccgccatcct tacagcagtt
     3061 actgaagccg gctgcggcct tacggcagaa tttcgcctca cccgtgaaag ccctgacatc
     3121 cacaccattc agggcggcaa catccttacc tgcggcggca agcgcagcca gctgtgatac
     3181 tgcttcacca aaatcactgt tttttccgct ctgtgcctta tcgcattcac cgtcaaggca
     3241 gaagacatca ccaccacaga ccatcacttt tcctgatgtt ctggactcgc aggagtacgt
     3301 ggcgtactca tgcagacagg ttccctcatc ggaataaaac gcgcactgac gtgaggccag
     3361 cgtacaggcc ggattatcga catatttctg acaggtgccg ttatctgcgg actgagtgac
     3421 atatttatcc cggtatgccc agcatgcctg tgtgatgttc cagggtttac cttccatcac
     3481 tccggttttc gtaccacccg gctctgaaca ctcagtgcct gtcagcacgc catccgcttt
     3541 attaaacgga caactctcca cccactccac ccggggaacc cattctttat cacggacctt
     3601 catcctgagt ttcagcgtaa aggtggaagc accactgaca agcgattcat agactttcct
     3661 gtcaccattc ccgtgcggga ggcaattacc gttagcagta cagccactac cggtcagaac
     3721 ctgcccctgt gtcacctgca aacctgtcgc cccggacagg gggtaagtat ccgactgccc
     3781 gtttgccagg ccgacattaa aggttgtatt catgaaggta taacgggagt tcaggaagaa
     3841 aaaagcggcg tatacagaca atgatgcact ttccactgta ccggttaccg gagccgtgac
     3901 actgaacacc agtttcagac cgttcatact gaaccggaac tgtgagtgcg gtatcgtcac
     3961 ctcacgatat tcatccgtgt atttccagtc cccggtgata ctggcagtcc gtgtgcagta
     4021 ctcctccacc atcgtgtccc gctcacaggt atagtttgtg aactcactgc ggtttatctg
     4081 ctgcgcactg cactgctgcc cggtgtttcc cacaatgctg tcagcccggt tcaccacatc
     4141 cctgccggtc tgaatgaacg gtgcatccgg tgacagaatg tctttcggct tgttcatgaa
     4201 ggactctgtg atggtttttc cggtttcacc ggtcgcccat tccgtggtgc cgtcattttt
     4261 caggccgcca tccccgccgg cagtcacgcc accgtaatat tttgtctcgt ccggattcgc
     4321 gttatagctg gggatactct cctgtggctt aaagccctga atactgcctg taccctgccc
     4381 ctgaatctga cgggcaaaat cagaaccggc ccggtaatca ctgttgctgt cagcctgtgc
     4441 catgccggca accagagcca gtatcagagg taaaatacgt ttcatttccc ggaatctcct
     4501 ttccctgcca gtaaatcatg agccacctgc cggcagtcac ccgtggtggc cactttctcc
     4561 agcgcctggc cgacccgcag gtttccccgg atgatgtcgt atccctgact gcagaacacc
     4621 accagtgccg gtacgctgcg aatgccgtac tgtgaaaaca gcgtcgggtc gatctgaaca
     4681 ccatcagttg cgccgtcttt caccagggac agcacggctt cagcggtggt cttcaggtca
     4741 ttattcacca tgccccgcag tgtggccgga ataccgtagt gccgggtttc gcccagcatc
     4801 cgtttcagcc cctcttcggg aatggaaaac gacacaaaat acagggcacc ctgccggggt
     4861 ttcccgtcct gcgaagcctg ctgtttacga accagctcat ccagaaaatg gttatctgaa
     4921 cgctgaaggg ggttttccag cacctgtttc tccgcccagg ctttcagctg atggtcaggt
     4981 ttctctcgca gttgtctgct taaattttcc tgctgcttca ggaactggcg gttttcaggt
     5041 gtgttcacat tttctgaagc atgaacagca agatgcatcc ctgctgtcag agcaaaaagt
     5101 gcaaatattt tccggttcat gatatttctc actttctgct atttacaggc atgaactcat
     5161 cagaaaaaag ataattgaga aaaacagcca taaacgcaca tgtaaatgtc accttcagat
     5221 ataatctgac aagtgtcact tgttctgata tatctgcctt cttccattca tgaataattt
     5281 cagcgagaaa actgtcaggt gcaaaataaa ataatataac gggcaccatt acaagaccaa
     5341 aaggaaataa ttttaatgtt ctgcttatca gcctgagcag cacagcactt attttcctga
     5401 ggtgctgaat atatttactg tccattgttg ccattttcat ttcctcttat tcattaaaat
     5461 ga
//
LOCUS       DAZFRG010000284         5453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFRG010000284 DAZFRG010000000
VERSION     DAZFRG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.286"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(162..362)
                     /locus_tag="ACHKUC_004410"
                     /pseudo
     CDS             complement(162..362)
                     /locus_tag="ACHKUC_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            466..2616
                     /gene="dinG"
                     /locus_tag="ACHKUC_004411"
     CDS             466..2616
                     /gene="dinG"
                     /locus_tag="ACHKUC_004411"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO6620538.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2644..3606
                     /gene="ybiB"
                     /locus_tag="ACHKUC_004412"
     CDS             2644..3606
                     /gene="ybiB"
                     /locus_tag="ACHKUC_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO6620539.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            3747..4832
                     /gene="hcxB"
                     /locus_tag="ACHKUC_004413"
     CDS             3747..4832
                     /gene="hcxB"
                     /locus_tag="ACHKUC_004413"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO6620540.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDEDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            4899..4958
                     /gene="ybiE"
                     /locus_tag="ACHKUC_004414"
     CDS             4899..4958
                     /gene="ybiE"
                     /locus_tag="ACHKUC_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO6620541.1"
                     /translation="MRLSANRQGNTCAERSVNA"
     gene            complement(5061..5321)
                     /gene="ybiJ"
                     /locus_tag="ACHKUC_004415"
     CDS             complement(5061..5321)
                     /gene="ybiJ"
                     /locus_tag="ACHKUC_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO6620542.1"
                     /translation="MKTINIVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         1290 a         1375 c         1527 g         1261 t
ORIGIN      
        1 caggcctacg tgaactctgc aatatattga atgtgcatgt ttttgtaggc cggataaggc
       61 gctcgcgccg catccggcat ttcacaacaa gcacttgtca gccatttgcg agcgcggcaa
      121 aaagatttct ctgttttaac gcccacaagc cacccgatgc gttacttctc cgcagccaat
      181 tgtccgcgca acgccattaa aaggtacccc aggcgattct tgccctgacc attaccgcca
      241 tctccccagt aagcatcatt ttgcgtatgc tcaaccagtt ttgcggacgc ggttgcccgc
      301 aagagtgtac gcagttctgc atgttgttcg aatttagcgc gaagcgcttt acgcatcatc
      361 ctgctggtta ttgcgagccg ctttcccgaa acagaaaaac cattacccct gaaaaccgaa
      421 aaatgccaca atattggctg tttatacagt atttcaggtt ttctcatggc attaaccgcc
      481 gcgcttaaag cgcaaattgc cgcctggtat aaggcgcttc aggaacagat ccccgacttt
      541 attccccgtg cgccgcagcg gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa
      601 gaagggcggc atctggcgat tgaagcccct accggcgttg ggaaaacgct ctcctatttg
      661 atccccggca tcgccattgc ccgcgaagag caaaaaacgc tggtggtaag taccgccaac
      721 gtggcattgc aggatcagat ctacagcaaa gatttaccgc tgctgaaaaa gatcattccc
      781 gatcttaaat tcactgccgc ttttgggcgt gggcgctacg tttgcccgcg taatctgaca
      841 gcgctcgcca gcactgagcc cacgcaacag gatctgctgg cgtttcttga cgatgaactg
      901 acgccgaaca atcaggaaga gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact
      961 tataaatggg atggtctgcg cgatcatact gatatcgcta tagatgatga tctctggcgt
     1021 cgtttgagta ccgacaaggc cagctgcctc aaccgcaact gttattacta tcgcgaatgc
     1081 ccgttttttg tcgctcgtcg ggaaattcag gaagcggaag tggtggtggc aaaccatgcg
     1141 ctggtgatgg cggcgatgga aagcgaagcg gtattgcctg acccgaaaaa tttactgctg
     1201 gtgctggacg aaggtcatca cctgccggat gtggcgcggg atgcgctgga gatgagcgcc
     1261 gaaatcaccg cgccgtggta tcggctacag ctggacttgt tcacgaaact ggtcgctacc
     1321 tgcatggagc agtttcgccc gaagaccatc ccgccgctgg cgatccctga acgtttgaat
     1381 gcgcattgtg aagagttgta tgagcttatc gcctcgttaa acaacattct caatctctac
     1441 atgcctgccg ggcaggaggc agagcaccgt tttgcgatgg gcgaactgcc agatgaagtg
     1501 ctggagatct gccagcggct ggcaaaactc accgagatgc tgcgtggcct ggcggagtta
     1561 tttcttaacg atttaagtga gaaaaccggc agccatgaca ttgtacgtct gcatcggttg
     1621 attttgcaga tgaaccgcgc attggggatg ttcgaggcgc aaagcaaact ctggcggctg
     1681 gcgtctctgg cgcaatcttc cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc
     1741 gaagggcagc tacacctttg gtttcactgc gtgggaatac gtgtcagcga tcagctggaa
     1801 aggctgctgt ggcgcagtat tccgcacatt attgttacct ccgcaacctt gcgttcgctg
     1861 aacagttttt cgcgtttgca ggagatgagt gggctgaaag agaaagcggg cgaccgtttt
     1921 gtggcactgg attccccttt taaccactgt gaacagggca aaattgttat tccccggatg
     1981 cgcgttgagc cttccatcga caacgaagag cagcacattg ccgaaatggc ggcctttttc
     2041 cgtgaacagg tggagagcaa aaaacatctc ggtatgttgg tgctgtttgc cagcgggcgt
     2101 gcgatgcagc gctttctcga ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc
     2161 gatcagccac gttatcgctt agttgaactg caccgcaaac gcgttgccaa cggtgagcgt
     2221 agcgtgctgg tgggcttaca gtcctttgcc gaaggtcttg atctgaaagg cgatctgctc
     2281 agccaggtgc atatccacaa gatcgctttt ccgcccatcg acagcccggt ggtgatcacc
     2341 gaaggtgaat ggctgaaaag cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc
     2401 gcttcgttta acctgattca gcaggttggg cgactgattc gaagccacgg ttgctggggg
     2461 gaagtggtta tctacgacaa acgcttgctg accaaaaatt atggcaagcg actactggat
     2521 gcattaccgg tatttccgat agagcaaccg gaagtccctg aaggtatagt taaaaagaaa
     2581 gaaaaaacga aatccccacg ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg
     2641 taaatggact atcgcaaaat cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat
     2701 ttagaccggg atactgcgcg cggtctgtat gcccatatgc tcaatggtga agtccctgac
     2761 cttgagttgg gcggcgtatt gattgcgcta cgtatcaaag gggaagggga agcagagatg
     2821 ctcggctttt acgaagccat gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag
     2881 ccgatgccga ttgtcatccc cagttacaac ggcgcgcgta aacaggccaa cctgacgccg
     2941 ttgctggcga ttctgctgca caaactcggt tttcctgtgg tggttcacgg ggttagcgaa
     3001 gatccaaccc gcgtgctgac cgaaaccatt ttcgaactga tgggtattac gccaacgttg
     3061 cacggcgggc aggcgcaggc gaagctcgac gaacatcaac ctgtctttat gcctgtcggc
     3121 gcgttttgcc cgccgctgga aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac
     3181 agtgcacata ccctggcgaa actggcgacg ccatttgccg aaggtgaggc actgcgtctt
     3241 tccagcgttt cgcatccgga atacattgga cgcgtcgcga agttctttag tgatatcggc
     3301 gggcgggcgc tacttatgca tggtactgaa ggtgaagtgt atgctaatcc gcagcgctgc
     3361 ccgcagatca atctcattga ccgtgaaggg atgcgggtac tgtatgaaaa acaggacact
     3421 gctggtagcg agttactgcc acaagcaaaa gatccggaaa ccacggcgca gtggattgag
     3481 cgttgccttg ctggcagcga accgattcct gaatcgctga aaatccagat ggcttgctgc
     3541 ctgatggcta cgggtgaagc ggcaactatc aacgacggcc tggcgcgcat taatcaggca
     3601 ttttaatttt tatctcagcc ggatgagcca tgtcatccgg cttttacccc accgcaatat
     3661 gaaattcctg catctttatt gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa
     3721 acacataaca taaacaggag ttaaccatgg aaagtggtca tcgctttgat gctcagacgc
     3781 tgcacagttt tattcaggct gtatttcgtc agatgggtag cgaggaacag gaagcgaaat
     3841 tagttgccga tcatttaatc gcggcaaacc tggcagggca tgattcacat ggtattggca
     3901 tgatcccaag ctatgtgcgt tcctggagtc aggggcatct gcaaattaat catcatgcca
     3961 aaatcgttaa agaggcgggg gcggcggtca cgctcgatgg cgatcgcgca tttggtcagg
     4021 tcgcggcaca cgaagcgatg gcgctgggga ttgagaaagc gcatcagcac ggcattgccg
     4081 ccgtggcgct acataactcg catcatatcg gccgtatcgg ttactgggcg gagcagtgtg
     4141 cagcggcggg gtttgtctct atccactttg ttagcgtggt cggtattcca atggtcgcgc
     4201 cgttccacgg tcgcgacagc cgctttggca ccaatccgtt ctgtgtggtt ttccctcgta
     4261 aagataattt cccgctgttg ctcgattacg ccaccagcgc cattgcattt ggcaaaaccc
     4321 gcgtcgcctg gcataaaggc gtccccgtgc cgccaggttg cctgattgac gttaacggcg
     4381 tgccgacgac caatccggcg gtaatgcagg agtcgccgtt gggttcgctg ttgacctttg
     4441 ccgaacataa aggctacgcc cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg
     4501 gcggtaaaac gacgcatcag gaaacgttac aaaccagtcc cgatgccatt cttaactgca
     4561 tgactactat cattatcaac ccggaactgt tcggcgcgcc ggattgtagt gcacagaccg
     4621 aagcctttgc cgagtgggtg aaagcctcgc cgcatgatga agataaaccg attttgctac
     4681 cgggcgagtg ggaagtgaac acgcgtcgcg aacggcagga gcaggggatt ccactggatg
     4741 cgggaagctg gcaggccatt tgtgatgcgg cgcggcagat tggtatgccg gaagagacgt
     4801 tgcaggcttt ctgtcagcag ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc
     4861 gaacaagggt aacataggat caatgagggt tagagcatat gcgtctgtcg gcaaacagac
     4921 agggaaatac ttgtgctgaa cgtagcgtaa acgcctgatc cagccaacgg ttcggatttt
     4981 gtaggtctga taagacgcgg tagcgtcgca tcaggcatct gttgccggat gcggcgtaaa
     5041 cgccttatct gtcctacgaa ttacttataa attaccgcag taccgcttaa tttattgttg
     5101 ttggtggcgg aagtaatgct gtatccactg gcaccggccg ccgcggcttt ctcagccagt
     5161 ttcgcttcca gggcatcgag ggtggatgcg ccatcggcag aaaccacgcc gattttattc
     5221 atgttctgtg cctgggatgc cgttaccggt tccgccgcga acacgccaaa tgacagagtt
     5281 gaaagagcca tagcagcaac aacaatattg atagttttca taattaatct ctcgcaggtg
     5341 atttttgtat aagtgacgtt gtttcgtcga tgtgatgagt atcacgtttt tttacgagag
     5401 ataaaatcga agagaattga ccgccttgtt caaataaatt gattgatatc taa
//
LOCUS       DAZFRG010000285         5448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFRG010000285 DAZFRG010000000
VERSION     DAZFRG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.287"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..1680
                     /locus_tag="ACHKUC_004416"
     CDS             124..1680
                     /locus_tag="ACHKUC_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6620543.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLLITLILGG
                     VFWQTPVMDMGSAVVYGTIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVAIIGGGMKAIRGVGLITL
                     MCGVSTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIDTSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFISTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            1730..2449
                     /locus_tag="ACHKUC_004417"
     CDS             1730..2449
                     /locus_tag="ACHKUC_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6620544.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            2460..3875
                     /locus_tag="ACHKUC_004418"
     CDS             2460..3875
                     /locus_tag="ACHKUC_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6620545.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFRDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            3880..4578
                     /locus_tag="ACHKUC_004419"
     CDS             3880..4578
                     /locus_tag="ACHKUC_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6620546.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSKALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMGAALWDPEKGDENIHIAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCA"
     gene            complement(4987..5228)
                     /locus_tag="ACHKUC_004420"
                     /pseudo
     CDS             complement(4987..5228)
                     /locus_tag="ACHKUC_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
BASE COUNT         1460 a         1160 c         1333 g         1495 t
ORIGIN      
        1 tcccaatttt ggaggcgata ctcattctcc ccctgataac taaacgctct cgtccgacag
       61 ggtgtggaga ctatcaatat ccacagtaat gatgctgact ttgttttcgt ttgaggaaat
      121 gacatgcacg attacctgct ttttttactg ggacttacac cgcttctggc gatgatcatt
      181 ttgattctga aagtcaaaat gcccatacat tatgccgtgc ttagcactct cctgatcacg
      241 ctaatccttg ggggtgtttt ctggcagacg ccagttatgg atatgggatc tgctgtggta
      301 tacggcacaa tcaaaggttt gtggccaatt gtgattgtta tccttgctgc catttacagc
      361 tataacctga tgaatgcgac aaaaagcatg gatattctga gaaatgttct ggcgagcgtt
      421 agtgatgaca agcgtatcca ggtacttctt atctcatggt gttttggcgg atttcttgag
      481 gcggcagccg gttacggtac agctgtagca atccccattg gtatactgat tgcactgggc
      541 tttaacccac taaaagcagc aattgcgtca ctggttgcta atacagtccc tacagcattt
      601 ggagcagtcg gtatacctgt ctctgtactt gctgaacaag ttaatttgcc tgtaaccaca
      661 ctcagtggga cgattattct tcagctggca ctgttcaaca ttctgctccc cttcgtcatc
      721 gtcgcgatta tcggtggagg gatgaaggct atacgaggcg taggattaat taccctgatg
      781 tgcggtgtca gcacattgat acctcagtat ctggtggctg ttaatcttgg tgcagaactc
      841 ccggctttcg cgggaagtct tgtcagtctg atcgttatag tctttatggc aaaaaaccgt
      901 aaagaaaaaa cagaacctga atttcttatt gatacttcag cagatagcgc cagcgcactt
      961 tcatactctc gtggggcatt gttacgtgcg tgctcaatct acatcctgat ttttctgttc
     1021 attctgcttt gctctccact gttcccgacg ataaaaagtg cggtgtctca gattgcctcg
     1081 tcactcagtt tctctctgag taacggcagc aatctgtcga tgaagattga atggatcgca
     1141 acccccggtg ttctgattat cattgccagt gttattggcg gcttgataca aaaagcatcc
     1201 ctgtcatgtc tgttaaacgt atttatcagt acggtaaaac agctcaaaaa ctccattatt
     1261 gccatttctg cgattgttgc catggcaacg gtaatggatg tttgtgggtt aattggcact
     1321 ctggcacaaa gccttgtgga tctcactggt ggagcctacc tcttcattgc tccggttatc
     1381 ggcgctcttg gtacatttgt tacaggcagt gacacaaact ccaacattct gttcggtaaa
     1441 ttgcagacca ctgcggctga aaaattgcat gtggacccgc tctggctggc tgccgcaaat
     1501 acatctggcg caacaggcgg caaaatgatt tctcctcaga gtattgctat cgctgtttca
     1561 gccacccgta tggatggtca gggcagcgta atcatgtcag gaacactgaa gtactgcttt
     1621 gcttatattg ttattcttgg actgaaagta ggccttgtct accacctgtt catggcctga
     1681 gaaaaaacaa caaaaggacg tggaatatta atcaacagga gaaatttctg tgaatgttaa
     1741 ctttttcgtc acctgcattg gtgatgcgtt taaatcccgg atggcacaaa agagtgtgct
     1801 gttactggag caattgggtt gcaatgtcca tttccccgaa aagcagggat gttgtggtca
     1861 gccagcgata aatggcggtt atgttcgtga tgccatccca ggcatgaaaa atctcatatc
     1921 ttcgctggaa gagaatgact ggccggtagt atctcctgcc gggtcatgca cttacacgat
     1981 aaaaaattac gctcgctatc tcagtgaaga acctgagtgg gcagcacgag ctgaaaaagt
     2041 ggctgaacgc ctgcatgatt tgacatcatt tatagtaaat catcttggca tcacagatgt
     2101 tggggcaaaa ctcaatggtc gcgctgtgta ccatccctcc tgcagccttt cccgtaaact
     2161 gggtgtaacc agagagccgc ttttgctact tgagaatgta caggggcttg agctactgcc
     2221 gtttcacgct caggaaacgt gctgcgggtt tggcggcaca ttctctgtaa aaatggcgga
     2281 gatctctggt gaaatggtga aagagaaagt tgggcatatt atggatgtta aacctgattt
     2341 tcttatcggg gctgacgcca gttgtctgct aaacatcggc ggtcggctta accgtgaagg
     2401 gcatcctgta aaagtgatgc atattgctga agtcctgatg cacagatgag gtaaacagaa
     2461 tgtctttgaa aacaagtgat atcaggttta aagagcgtat ccgtattcag attcaggacc
     2521 cgatcatgcg aaaagctgtg gctaacgcac aggagcgcat agggactaat cgacagaaaa
     2581 tggttgatga actgggacac tgggaggaat ggcgttcacg agctgaacag attcgtcggc
     2641 atgttctgga aaatcttgat gcttatttgt atcaattgtc cgaaaaagtt accgcgaatg
     2701 gcgggcatgt atattttgcc cgtaccagag aggatgcaac cagttacata ctgaaggtgg
     2761 cgaaagaaaa aaacgccagg aaggttgtta aatccaaatc aatggtgact gaggaaattg
     2821 gcatgaatgc cgttcttcag aaagccggga tacccgtgat tgaaacggac cttggggaat
     2881 atattcttca gctagcacat gagccgccat cgcatgtggt tgtgccggca atacataagg
     2941 atcgccatca aatccgcaga gttttacatg aacatctggg gtatgacggg cctgaaactc
     3001 cagaagcaat gacattgttc atccgtgaga aaatcagaca agattttctg agtgctgaag
     3061 ttggcgtaac gggatgtaac tttgccgttg cagaaacagg gtcagtttgc cttgtgacaa
     3121 acgaaggcaa cgcacggatg tgcaccacat tacctaaaac acatattgct gttatgggta
     3181 tggagcgtat tgctccgaca ttccaggagg tggatgttct gattaccatg cttgcaagga
     3241 gtgctgtcgg ggcgagactg acaggttata acacctggct tacaggcccc agagaagaag
     3301 gacatgttga tggtccggaa gagttccatc tcgttattgt tgacaatggg cgttcagaga
     3361 ttctcggctc agaattcagc gatgtattac gatgtatccg ctgcggggct tgcattaata
     3421 cctgcccggc ttatcgtcag attggtgggc atggttatgg ttcaatctac ccggggccaa
     3481 tcggcgcggt tatttcccca ttgcttgggg gatatgaaga ctttagggat ttaccctatg
     3541 cctgctcatt gtgcactgcc tgcaatgggg tatgtcctgt aaaaatacct ctggcaacgc
     3601 taatacaaaa acatcgccag gttatggcag agtctgaaat cacaccatta acggaacgcc
     3661 gtataaccag agcattcaat tacataaaca gccatccggc gttgtggaag gtaggtatgg
     3721 ttgctggggc caaaacagcc cgtctgttca tcaggaaagg aaaaacgccg attagtgttg
     3781 gagcgataaa agagtggact gaagccagag acctgccatc acctgatgga gaaagcttcc
     3841 gtagttggtt caagcgacat aagaaggagg ggtaatttta tggaaaacag agaggaattt
     3901 ctcgttgcga tcgccagaaa gcttggcaga ccagtaagac atattcccga agcaatgccg
     3961 gagcctgtaa atacgctggc tacaacacgt ttaacagagt tgacatcaga ccaacgctgt
     4021 gaggcgttta ttaaatttgc ctcagaagtg atgcttgccg agtgtgttct ggtgtcgcct
     4081 gaaaacgcac catcaaaagc cttagacatc tgttggaagt tcggttccgg gccagtcatt
     4141 atcagcaacg atcaacgact ggtcgataca gggattacgc cattgctaca aaaagaaatg
     4201 ggcgctgctc tgtgggatcc ggagaagggc gatgaaaata tccatattgc tgaaaaggca
     4261 aaaactggtg tcgttttcgc tgagtatggg ctgacagaat caggaggcat tgtcttattc
     4321 tccgcaccag aacgtgggcg ctcagtaagc cttttgcctg aagcatcatt atttgtttta
     4381 cgtaaaagca ccattctccc cagagtggcg cagcttgcgc aaaaacttca tgatatggct
     4441 caggaaggac aacggatgcc gtcctgtata aatctgattg gaggcccaag ttctactgca
     4501 gatatagaac tgatcaaagt tgttggcgta catggtcctg tacatgcaac ctatttgatc
     4561 attgatgatt gcgcttaacg attgctaata aaaaaatggc cttaaggtga tattgcaatc
     4621 gtcttgtgaa aaggcaacaa tagactaaag gttaggcata attgtgtttc tttatgcctc
     4681 cgcagcaatt tgtggaggct tttttgctta ctccaaacag tttggcttct tgaaaaagtc
     4741 atatcccaaa cctattgacg cttataaaaa agtaaagaat ggtgagtaag cgtaaactga
     4801 ccgccgtatg tagccatgat gccagtattg gtaacgtagg tgcaccccta tctatttttg
     4861 tagaaccgga ggaaacagac cagatagttt aaatgagctg gttatgcgta acataattta
     4921 tctattttgg ctcttacaaa atagataaag aaggagtatc tgatgagata ctccattatg
     4981 acagactcaa ctcatgtgat ttttcttaaa gttctctgct tgcgccagtc ccccatataa
     5041 acatcatcat tcgctactgg cgggaactta tgcttgtgta gaagcagaat aagttcaact
     5101 ttcagtttcg ctttaatatc atcgcgttta ctccagtcag gatatttcga tgtgttgtca
     5161 accacgcttt tcatctcttt tgccagcgac agcatttttt catcgtcata ggtgaactga
     5221 tatttatcgg taagcgttaa cggagcgccg tattgacact tatttattgg tgagtactac
     5281 gttccatggg taagcgtaca gcctgaaccg tctggtcaga atctgacgaa ttagacaaag
     5341 tggtgtccac caaataagta gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc
     5401 ccggcaggcg aaaaggctgc tctaattatc ctcccgaatt taaacagc
//
LOCUS       DAZFRG010000286         5446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFRG010000286 DAZFRG010000000
VERSION     DAZFRG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.288"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..1002
                     /locus_tag="ACHKUC_004421"
     CDS             157..1002
                     /locus_tag="ACHKUC_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6620547.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLK
                     KQAEDLIQSTQFFNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(1032..3029)
                     /gene="tkt"
                     /locus_tag="ACHKUC_004422"
     CDS             complement(1032..3029)
                     /gene="tkt"
                     /locus_tag="ACHKUC_004422"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6620548.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGRFAEDTAARFRAYGWHVIEGVDGH
                     DPEEVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(3080..4588)
                     /gene="ptsG"
                     /locus_tag="ACHKUC_004423"
     CDS             complement(3080..4588)
                     /gene="ptsG"
                     /locus_tag="ACHKUC_004423"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO6620549.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMAKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            complement(4630..5187)
                     /gene="hxlB"
                     /locus_tag="ACHKUC_004424"
     CDS             complement(4630..5187)
                     /gene="hxlB"
                     /locus_tag="ACHKUC_004424"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFO6620550.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
BASE COUNT         1472 a         1456 c         1204 g         1314 t
ORIGIN      
        1 cgtttaaatt atttttgtgc agttgacgta attcatgaaa attaaccggc ctgttaatca
       61 ttacgttcac tgcacacctt catttttttc acacctatcc acgcctgaaa actatgttat
      121 aatcagcact ttctgtcaga atgaaattta ctaattatga ataacataac cgtaaaaagt
      181 ctgattcaaa gcaattaccc tacactgcat caggcggaaa aaaaggtcgc tgactatatt
      241 cttagccacg cacatgaagt cgtgaactat agtgtcactg aactatcaga aaaaagccat
      301 gccagtgaag cgactatcgt acgcacctgt aagaaattag gttatcaggg ttattaccat
      361 ttaaaaattg cattagcaaa agaagtcatt aatcctgaca atagctatcc tgaaaatacc
      421 gacttcagcg atataacatc tttggcaacc ttcttattaa aaaaacaggc cgaagattta
      481 atacaatcaa cgcaattttt taatgccgac gtgcttgagt ccattctcaa actacttgct
      541 aattgcgata ctattttctt ttttgctgcc ggaaattcaa acccactggc cgtttatagt
      601 gcttataaat tcagccaact cggattaaaa accgttgttc acgtcagccc ggaaatgcaa
      661 attaatgcgg catattctat ggggaaacgc gacctggcga ttggtatttc taactctggc
      721 agcaccaact taatggtcga tatctttaaa gtggtgaaag agcgcggtgc caaatccgtt
      781 tgtatcacta actatattaa atctccgtta tcaaaactct ccacccatca actcaatacc
      841 gcggtcagcg ataaaatctt ctttgaagcc ttcgactcca ctcgcgttcc ggcaatgggc
      901 gttattgata tgctggtgct gttgtttatg tataaaaaca aggcgcatta cgagaaatac
      961 cgcacccgtg aagagtttct tggctcccgt ttcaaggggt aaaaggtcat cctttcaccc
     1021 cttgtgcagg tttagctgtt cagtagctca cgcgcctgcg ccaggacatt ttccggcgta
     1081 aaaccaaagt gtttgaacaa cacattcgcc ggagcagact caccaaagct cgtcatgccg
     1141 atcactttac cgtccaggcc gacgtagcgt tcccagaatc cggcgatgct ggcttctacc
     1201 gccagacgtt tgcgtacagc ttttggcaat accgattctt tgtacgccgc atcctgatta
     1261 tcgaaacgct cggtacacgg catagaaacc acgcgaactt tacgcccttc ttccgttaac
     1321 gcttttgcgg cgctgaccac taactcaatc tctgaacccg cagaaatgag gatcatctca
     1381 ggctgaccgt cacaatcact cagtacataa ccaccacgtg caatctctgc caactgtgca
     1441 gcggtgcgcg gctgttgcgc cagaggttgg cgcgttaata ccaacgacgt tgggccatct
     1501 ttgcgctcaa tcgcctgttg ccaggccacc gccacttcca cctgatcgca accgcgccag
     1561 gtttccatat tcggcgtcag acgcagcgac gccagttgct caacgggctg atgtgtcggg
     1621 ccgtcttcgc ccaggccgat ggtatcgtgg gtataaacaa acaccgagcg gatcttcatc
     1681 agcgccgcca tgcgcagtgc attacgcgca tattccataa acatcaggaa agtaccgccg
     1741 taagggataa acccaccatg cagcgcaaga ccgttcatga tggccgacat accaaattca
     1801 cgcacgccat aggagatgta attccccgcc ggattttctc cggtgaaatc aaccgatttc
     1861 tgatggcgag tcaggttaga gggcgacaag tccgccgagc cgcccatcag ttccggcagc
     1921 atatctgcga agtgattgag gcatttctgg ctaacctgac gggttgccag cgccgccgga
     1981 tgggattgca gatcgtgcac atatttctgc atattctcga cccagccagc aggcaaatca
     2041 cccttcatgc gacgagtaaa ctccgccgcc agttccggcc actgcttcgc gtagtcggca
     2101 aacagtgcat cccattcctg ttgaacctgc gcgcctttct cggtcgcatc ccattcggca
     2161 taaatttcac caggaatttc gaaaggggca tacggccatt gcagacgttc acgcaccagc
     2221 gccacttcat ctgcacccag cgccgagccg tggcaatcgt ggctgttggc tttatttggc
     2281 gaaccaaaac cgataatcgt tttgcagcac agcagactgg gcttatccgt gacgcttttc
     2341 gcttcacgca ccgcagcatc tacctcttct ggatcgtgac catcaacgcc ttcaatgaca
     2401 tgccagccgt aggcacgaaa acgggcagcc gtatcttcag caaacctgcc ttctacgtga
     2461 ccatcgatcg aaataccgtt atcgtcccag atagcaatca gattgccgag cttcagcgtt
     2521 cctgcaagac cacaggcctc gtgggagatc ccttccatca ggcagccatc acccaaaaat
     2581 agccatgtgt gatgatcaac aatattaaag cccggtttgt taaattctgc cgccagcgcc
     2641 ttctccgcaa tcgccatccc caccgcatta gctacgccct gccccaacgg gccggtggtg
     2701 gtttcaacac ccggcgtata accatattct ggatgacccg gcgtgcggga gtggagctga
     2761 cgaaagtcgc gaatatcatc catcgacagg tcatagcctg taagatgcag caaagcgtaa
     2821 atcaacatgg agccatgacc gttcgactga acatagcggt cgcggttaaa ccactgcgga
     2881 ttttttgggt tatgacgcag gtgacgacgc cagaccactt cggcaatatc cgccatcccc
     2941 atcggcgcgc ccggatgacc ggagttcgct ttctggatcg catcaacact taacattcgg
     3001 atggcattag ccagtaattg tgaattcata cttcagctct ctctgtctgg cgggctgaaa
     3061 ccagcccgct aatctcgaat tagttagcac aggccactgt catttctgtc gggcgcggtc
     3121 tgctcattac ggttttgatt tcgtcgcgca ggatttgcga tttgccgcca aatatcacct
     3181 gtacaccgcc atcacccagt ttcactaaac ccgcagcccc caactccttc agccgttttt
     3241 cattcacttt cttatcgtct accagcgcca gccgcaggcg tgttgcgcag gcatccacat
     3301 cttcgatatt gtcgaaacca cctactgctt cgatgatttg gtatggacgt tcttcgcgcg
     3361 ttaatgtggt tgcaacggca atctcttcat cctcacgtcc cggtgttttc agattcaact
     3421 tgcggatcag tacccgaaat acggtgtagt aaatcgctgc atagcccgcg cctgccagga
     3481 acaccagata ccacggcgtg cggtttggca taacgccatt cagtacgaaa tcaataagac
     3541 cgccagcaaa aggatgacca atgtgcacat tcaggatgtt catcagcaca aatgaagtgg
     3601 catataagaa gatgtgaatg atgtagagaa ccggagctgc aaacaaaaat gcatattcca
     3661 gcggctccgt aatcccggta acaaatgtgg tcagcgcacc ggagagtaaa aggcctttaa
     3721 tcagcgcctt acgttcaggt tttgcttcct gatacatcgc caacgcggcg gcaggtaacg
     3781 caaacatcat gatcgggaaa cgacctgtca taaacagccc cgccgtcaac ggaacaccgt
     3841 cgcgcaattg ggcaaagaac accggaatat cgcccgtcac cagttgcccg gatttagcca
     3901 tgtagtcgcc aaagttgtaa taaaacggca cgttccagat atgatgcagg ccaaaaggaa
     3961 tcagcgaacg ttcgccaacg gcgtaaataa aggccgcgaa gttcgcgtta tgcccactgg
     4021 tcaccagcgt tgaaagcgac gccagacctt tttgtaccgg tatccacagc cacggcaata
     4081 aatagccgac aataattgcc gccaccgccg taataatcgg tacaaagcgt tttccaccga
     4141 agaaaccaag aaactgaggc aattcaatat tgtggaagcg ggtatagagc agataaacaa
     4201 agatggcgac aatcaaaccg ccaaacactc ccgtttgtaa cgtcggtacc ccgaggatca
     4261 tcgcatagtc accgttactg gctgcaacgg actccgcggt aatgccaagt tgcgtaccca
     4321 tcgtggcatt catgatcatg tagctgataa tcgctgaaag tgaagaagaa ccatcacctg
     4381 tcaggccaat cacaataccg acacaaaaca gcaaaggaag gttggcgaaa atcaggccgc
     4441 cgcaattcgc cagcagctca atgttaaatg aaatcccgat ggctaaaatc aaacccgccg
     4501 ccggaagaat ggcgattggt aacatcagag cagaagaaaa tttctgcaac ttcttgaagt
     4561 taaacaagga tgttattttt ttgctcattt ttattctcct gtagggtatc gagcaaataa
     4621 cggcgacgac tattccagat tggcgtggcg cttaaacagt tcactttccg gcgcaccact
     4681 ttcagcagcc atcttcatca ctaatgcatc ggcaatcacc agcaaagctt gctcatacaa
     4741 cgacgccatc ggctgtgccg agcggaaaac agaatctttt tgatttttgg aaggggcatt
     4801 aatttgcacg atgacatcga caaactcggt gatagtggcg tgtcgattcg tggttaacac
     4861 cgccacagcg ccgccaagct gacgtgcttt tttcgccagt gccaccagtt gtgctgtctc
     4921 accggaagcg gaagaaacga taaacagatc gccaggccca aagttcggcg taatggtttc
     4981 cgtcaggaag taaacctctt ttcccatatg catcaggcgc atggtgaagg ctttcatgga
     5041 aaagccggca cgtcccaggc catagcagaa aacgcgctgg ctttgctgaa tagcgtcgat
     5101 caatttggtg cagtcatcct cacggataga gtccttaacg aaggttaatt cattgatgat
     5161 ctcagtgatg tacagctttg cgtccatttc acacctcatg aaataatttt tctcaaacac
     5221 aaaatctcat gaaattttat ttcattccaa tgcatcagta tgaaatgtga agctagtcac
     5281 aagaaagaga gtagattgta tataactatt tgaatttaag aattatttct ctttttaaga
     5341 aagataagct tgaggggaag gtgaaatcag ccataaaatt ttgcggctgt aactgccctg
     5401 taaaaatcac aaactgaact cgtctctggc tatacaccga acaact
//
LOCUS       DAZFRG010000287         5396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFRG010000287 DAZFRG010000000
VERSION     DAZFRG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.289"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..1390)
                     /gene="aroA"
                     /locus_tag="ACHKUC_004425"
     CDS             complement(107..1390)
                     /gene="aroA"
                     /locus_tag="ACHKUC_004425"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO6620551.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIHIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLENMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTMLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1461..2549)
                     /gene="serC"
                     /locus_tag="ACHKUC_004426"
     CDS             complement(1461..2549)
                     /gene="serC"
                     /locus_tag="ACHKUC_004426"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO6620552.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVVAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2748..3440)
                     /locus_tag="ACHKUC_004427"
     CDS             complement(2748..3440)
                     /locus_tag="ACHKUC_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO6620553.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3570..5330
                     /gene="ycaO"
                     /locus_tag="ACHKUC_004428"
     CDS             3570..5330
                     /gene="ycaO"
                     /locus_tag="ACHKUC_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO6620554.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1398 a         1381 c         1269 g         1348 t
ORIGIN      
        1 acgctaccat taataaaaat accccacaag gtccgaaaaa aaacgccggc aatgtgccga
       61 cgtctttgaa aagaaaggat tgtctatgtt atcgcccgtt attcattcag ccgggttgac
      121 ttatccgcgc caattgctcg aaatagtctg gaaaagtttt agcggtacat tttggatcaa
      181 gaatcgtcac aggcgtatct gataatgcga ccagcgagaa acacatcgcc atccggtgat
      241 cattataagt ggcgatctca gcaaatttca gtttttccgg tggcgtaatg cgaataaaat
      301 cgtgcccctc ttcgacttcc gcaccgactt tacgcagttc tgttgccatc gcaaacaggc
      361 gatcggtctc tttaacacgc cagttataga tattgcgcag catggtggtg ccttttgcaa
      421 ataacgccgc ggtggcaatg gtcatcgccg catcgggaat atggttcata tccatatcaa
      481 tggcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa atggtcgcac
      541 ccatattttc cagaacatca gcaaagcgaa tatcaccctg catactgttg cgtccaatgc
      601 cggtcacttt tacagtgccg cctctgattg ctgctgctgc caggaaataa gaagccgagg
      661 atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccc ccttttacga
      721 caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc agattgaggg
      781 tgatatcgat ataaggttta gaaaccagat cgcctttaat atgaatcacc gtatcttccg
      841 gcgcaagagg cgcagtcatt aacagtgcgg tgaggaactg gctggaaacg gagccatcaa
      901 catcaacgtt gccgccagta aagccgccct gtaaacgcaa tggcggataa ttttcttgtt
      961 ccaggtaagt gatcttcgcc ccgccctggc gcagcgcatc caccagatga ccaatcgggc
     1021 gttctttcat tcgcggttca ccggtcagta caatatcatt gctacccagg caaagtgctg
     1081 ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactcacgg gcactttctg
     1141 cgtgtaatgg accaccgtta ccgataattt cgcaacgcgt acgatcggct gaaagcgtat
     1201 agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg ctatccagca
     1261 gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg cggttagaaa
     1321 cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt tgtaacgtta
     1381 gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc tggctgtggg
     1441 gattatgcaa aatttcgaca ttagccgtga cggcgttcga actcaaccat gaagtctgtc
     1501 agcgctttaa cgccttccag tggcatggcg ttataaatag acgcacgcat tccgccgacc
     1561 actcggtggc ctttcagcgc atgtaagccc gcagcgaaag actcttcgag gaacaattta
     1621 tcaagcgcgc tatccgccaa ctggaacggc acgttcatcc gcgaacggtt agctttcgcc
     1681 acatcattgc ggtagaaatc gctgttatca atcaccccat acagcagttc tgctttttgc
     1741 tgattgatct tatccattgc aaccacaccg ccgttcgctt tcagccattt gaagaccagg
     1801 ccagataagt accaggcaaa tgtcggcggc gtgttgaaca tggaatcgtt atcgttgagg
     1861 atggagtaat ccagaatcga tgggcaggcg atattcgctt tgcccagcaa atcttcacga
     1921 acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc gtaaattaca
     1981 ccataacggc tgacatcaat cggacgggaa agaatggttg aagagaagtc agcggcgacc
     2041 accacgtctt taccgaagtc tggtgtttca tcgatagcga taccgtcaat ggtttcgttc
     2101 gggcagtaat gcatataagc agcattatca gagagttgcc attcactcat tggcttaact
     2161 gcgcgaagac catcaacggt cactttcgca tcaaacacat ttggtgtgca gtattttttc
     2221 gcttctttga tagcacttgc tgcccagtaa cccgcatcaa cataatctgc agtggtttta
     2281 tcaccaagaa tattcagcgg tacagcagca aattgaccgc gaccaccgcc atggcagaac
     2341 aataccttgt agttggaggg gacgttaaga agatcgcgaa aatccttctc ggcttcctct
     2401 gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt accaagaccg
     2461 ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag cattgccgga
     2521 ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt gccattaacc
     2581 cgcggtcaat caattgcgac cacaggcaat acattattcc tgtggctatc ggttttatca
     2641 ttcagtgaca cgcatcgcaa tgagtaaaac cgttgagggc aaaaatgagg cacgcgtcac
     2701 acaaaagaat ctctcggatt attttcaaaa aaccgatgtt ttggctgtca ggtcacccgt
     2761 ttagcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa ttgtttttcc
     2821 cccgcgttca tatgaatgat ttcactgcaa cggatgcagg catagtccgc cgactccggc
     2881 accactttgt aacgctgagt acagtcactg ggtaaaataa gtaaccccgg tttcaccttg
     2941 tcatcttcaa agaaataaac actgatttgc ccgttggttt cgagaatcgc cagacgtacc
     3001 tgccccaact gctctacacc gcgcaatcgt agctccataa agaactcaaa ttccgtcatg
     3061 ttggagttat tgagtttcga ccaggccagc tcgccatctt caataatgac aaccggcttg
     3121 ccttccagaa gatcttccag tttttcactg tgcgccatca accacattac caggcggtat
     3181 aacaacgcca gagtaataaa gacgataagt accgggacca tcggcacatc atcataaaac
     3241 gccacatctc ccgccgctga acccagtgtc agaatgatta acacttcaaa cagcgacatc
     3301 tgccgcacac cgcgtcttcc ggtcattttg aggaacaaaa agaccagtac aaaggtataa
     3361 agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcgaa tgccatacgg
     3421 tggagatcaa acgccttcat ttttatgctc ttatcgtagt taatccgcta aaagcatagc
     3481 taacagttca tcagcgtggc ggcggtgata gcacctttga gataaaaccc gtattattgc
     3541 gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc ggcaaagatg
     3601 ccgctctgga agattccatc gcacgcttcc agcaaaaact ttcagacctc ggttttcaga
     3661 ttgaagaggc ctcctggctg aatcccgtgc ctaatgtctg gtctgtacat attcgcgaca
     3721 aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg gcgctggctt
     3781 ctgccctcgg tgaatatttc gaacgtctct caaccaacta cttttttgcg gacttctggc
     3841 tgggcgaaac catcgccaac ggcccattcg tgcattatcc caacgaaaaa tggttcccac
     3901 tgaccgaaaa tgacgatgta ccagaagggc tgctcgatga ccgtctgcgc gcattttacg
     3961 atccggagaa tgaactgacc ggtagtatgc tgattgacct gcaatccggt aacgaagatc
     4021 gtggtatttg cggtctaccg tttacgcgcc agtccgacaa tcagaccatt tatattccga
     4081 tgaatatcat tggtaacttg tacgtttcta acggtatgtc tgctggcaat acccgtaacg
     4141 aagcacgcgt tcaggggttg tccgaagttt tcgaacgtta cgtgaaaaac cgcattattg
     4201 ctgaaagcat cagcttgccg gaaatcccgg cagacgtgct ggcacgttat ccagcagtag
     4261 ttgaagcgat cgaaacgctg gaagcggaag gtttcccgat cttcgcttat gacggttcgc
     4321 ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac ggtacctgct
     4381 ttgcctcatt cggtgcgcat cctgattttg gcgttgcact ggaacgtacc gtgaccgagt
     4441 tgctgcaagg tcgtggcctg aaagatctgg atgtgtttac tccaccaacc ttcgatgatg
     4501 aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc ggtttaatct
     4561 cctgggacct gttcaagcag gatgccgatt atccgtttgt tgactggagc ttctccggca
     4621 ccacggaaga agagttcgcc acgctgatgg ctatcttcaa aaaggaagat aaagaagttt
     4681 atattgccga ttacgagcat ctgggtgtct acgcttgccg tattatcgtg cctggcatgt
     4741 ccgatattta tccggctgaa gatctgtggc tggcgaataa cagtatgggc agccatttac
     4801 gtgaaacgat tctttcgcta ccaggtagcg agtgggaaaa ggaagattac ctgaacctca
     4861 tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgaa ctgttgggtc
     4921 tggcgaccgg gtcggataac ggctggtaca ctctgcgtat cggtgaatta aaagccatgc
     4981 tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg acgatggagt
     5041 ttaactcatc agtattcagc ccggaacgcg ccaattatta tcgctgcctg caaacgttgt
     5101 tattactggc gcaggaagaa gatcgccagc cgctgcaata tctgaatgcg tttgttcgca
     5161 tgtacggcgc agatgccgtg gaagccgcca gtgcggcaat gagcggcgaa gcggcatttt
     5221 atggcttgca accagtagat agcgatctgc acgcgtttgc tgcacatcag tcgttgttga
     5281 aggcctacga aaagctgcaa cgcgccaaag cagcattctg ggcaaaataa aatcaaatag
     5341 cctacgcaat gtaggcttaa tgattcgtct gagttatatt acggggcgtt ttttta
//
LOCUS       DAZFRG010000288         5372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFRG010000288 DAZFRG010000000
VERSION     DAZFRG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.290"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1447
                     /locus_tag="ACHKUC_004429"
     CDS             <1..1447
                     /locus_tag="ACHKUC_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO6620555.1"
                     /translation="ATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQS
                     LLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPE
                     HYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGL
                     SQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITI
                     PVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGV
                     QALHKVSMQHYDLLITDVNMPNIDGFELTRKLREQNSSLPIWGLTANAQANEREKGLN
                     CGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDIEALKNNTANDLQLMQEILMT
                     FQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDD
                     SKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(1503..2648)
                     /gene="yfdE"
                     /locus_tag="ACHKUC_004430"
     CDS             complement(1503..2648)
                     /gene="yfdE"
                     /locus_tag="ACHKUC_004430"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO6620556.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(2722..3666)
                     /gene="yfdV"
                     /locus_tag="ACHKUC_004431"
     CDS             complement(2722..3666)
                     /gene="yfdV"
                     /locus_tag="ACHKUC_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO6620557.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(3736..>5372)
                     /gene="oxc"
                     /locus_tag="ACHKUC_004432"
     CDS             complement(3736..>5372)
                     /gene="oxc"
                     /locus_tag="ACHKUC_004432"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO6620558.1"
                     /translation="NNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAAS
                     GFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELD
                     QMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEA
                     LTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIILGKGAAYSQSDEQLREFIESA
                     QIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGKKGWAADT
                     QFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFTTPLVWRDILNIHKQQ
                     NAQKMHEKLSTDTQPLNYFNALGAVRDVLRENQDIYLVNEGANTLDNARNIIDMYKPR
                     RRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFGFSGMEIETICRYNLPVTIV
                     IFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTT
                     GIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
BASE COUNT         1565 a         1293 c         1132 g         1382 t
ORIGIN      
        1 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
       61 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
      121 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
      181 gggtaactat caacttcaac cacaatgggt cgatattcct actttagtcc agaacacttg
      241 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacgtt
      301 tcctgaacat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
      361 actgagtaat gctctcaaat ttactaccga gggggcagta aaaattacga cctccctggt
      421 tcacattgat gacaaccacg ctgtaatcaa aatgacgatt atggattctg gaagtggatt
      481 atcacaggaa gaacaacaac aactgtttaa acgttatagc caaacaagtg caggtcgtca
      541 gcaaacaggt tctggtttag gcttaatgat ctgcaaagaa ttaattaaaa acatgcaggg
      601 tgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
      661 agaaattacc caacaagtgg cggctgtcga ggcaaaagca gaacaaccta tcacactccc
      721 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
      781 ccagctaaat ctattaggat atgatgttga cgaagccact gatggtgtgc aagcgctaca
      841 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatataga
      901 tggttttgag ttgactcgca aactccgtga gcaaaattcg tccttaccca tctgggggct
      961 tacagccaac gcacaggcta acgaacgtga aaaagggtta aattgcggca tgaacttatg
     1021 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     1081 tgcgcatatt gtacctcagt atcgccacct tgatatcgag gccctgaaaa ataatacggc
     1141 gaacgatcta caactgatgc aggagattct catgactttc cagcatgaaa cacataaaga
     1201 tttacccgct gcgtttcatg cactagaagc tggcgataac agaactttcc atcagtgtat
     1261 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     1321 ccagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     1381 ctctgtaaaa gaacacattg cagagctgga ccaggagatt actgttttct gtcagcaaaa
     1441 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     1501 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     1561 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     1621 cccccggctt caatcaacat atttctcgcc tgagtttgtg gtaaattaat ggcctcagcc
     1681 acacttaata acggcgcgac gggcacacca acttcatgta ttctggctaa ccaaacttca
     1741 gctacctgcg tttttaacgt ccgctcaata tattctttaa gaatagcctg gttatgtacg
     1801 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     1861 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     1921 tcgaaaacat caaaaggtgc catgtaggga tggcgatttc ccaggcgttg tggtgacttc
     1981 ccagtcgcga tatatgccat cagtccatgc tccagaaaac tcagagtggc atcaaacatc
     2041 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     2101 ccactgaata aataaacacc gccacagaga tccgcaagag aggtaccaac gcgcactggc
     2161 ggagcatcag ggtatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     2221 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatata
     2281 aggcgcgggt tgatttcttg taacctttcc catgaaaacc cgagtttttc cattgtacct
     2341 gggcgaaaat tctcagctaa tacatcagct tgtttaagca tatttataaa tatactttta
     2401 tcgtgatcat tctttaaatc aagaaccaca ctctcttttc catgattaat aaaactgtaa
     2461 tagagcgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     2521 ggctcaactt taattaccct tgcacccata ttacaaagaa gttgagttcc gaaaggccca
     2581 ttaaggacat gcgtcatatc gataactaat aagccttcaa acggcccttt gcattcatta
     2641 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     2701 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaaaggagc
     2761 cgtgacgaca aaacccagta cgctcacagc caatgacgct gtaccagtgc gggtgtagac
     2821 attaaaccgg ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac
     2881 catcatctgc aaatgttcgc tattcaaatg acatgccata cctacgagaa gcagcgccag
     2941 tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt
     3001 atgtgcagcc agagtcaacc ccgcagcgaa taccgccacc cctgagttag ccttcgcaat
     3061 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
     3121 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt
     3181 actattcttc ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat
     3241 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
     3301 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
     3361 taatgcacat actgctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
     3421 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcaggc gagtgtccgc
     3481 aaaaatcatt tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta
     3541 gtttaatacc agtttattaa atgcccgagc ttgatcctct gaaaaagttt ctcgtctgcc
     3601 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
     3661 tagcataata gtatttccga taagggtccc cttaccatcg cataataagt tggcaagagg
     3721 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
     3781 actttcagtt cctgctgctg ggtcgatgac cacattaata atggtcggtt tgcgcgactg
     3841 aattccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
     3901 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggcgatgg
     3961 tgcaccagcg ccgctgaggt caacaccgtc tcctctgtag atgccgccat tattaaaaat
     4021 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atctccatcc cactgaaacc
     4081 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaggtcacgc tagcaccgat
     4141 ggcatagccc ataccgatgc ccatgacacc ccaggtgcca caatccagac gacgacgtgg
     4201 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcccctt cattaactaa
     4261 ataaatatcc tggttctcgc gcaaaacatc gcgcacggca cccaatgcat taaagtaatt
     4321 taatggttgg gtatctgtac ttaacttttc atgcattttt tgtgcatttt gctgcttgtg
     4381 gatatttaaa atatcgcgcc acaccagtgg agtcgtgaat gtgttttgtt tcaattctgc
     4441 cagcatacct tgcatactgg atgcaatatc gccaacgact ggcacagcaa tggggcggtt
     4501 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
     4561 ttttttaccg tgtgccagta accaattcag tcgtgcacca acaagcatga caacgtcagc
     4621 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcgaggat
     4681 ccctttcgcc atagacattg gcaggaatgg aatctgggca ctttcaataa attcacgaag
     4741 ctgttcatca gattgtgaat acgccgcgcc tttgccaaga ataattaatg gtcgttcagc
     4801 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
     4861 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
     4921 gacatttgct ggcaaatcaa gataaacacc gccaggacga cccgatactg agacccggat
     4981 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
     5041 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
     5101 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcatt
     5161 ggccaatgcg gtcaaaccgt tgaggaatcc tggcgcagac actgtcaggc agatccctgg
     5221 tttttgggta agaaaaccgc ttgctgcagc ggcatagcct gccgactgct catgacgaaa
     5281 accaatataa cgaatgcctt ccgcctgggc atggcgcgcc atatccgtca caggaatacc
     5341 tacaacacca taaatagtgt caatattatt ct
//
LOCUS       DAZFRG010000289         5369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFRG010000289 DAZFRG010000000
VERSION     DAZFRG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.291"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            290..2995
                     /gene="malT"
                     /locus_tag="ACHKUC_004433"
     CDS             290..2995
                     /gene="malT"
                     /locus_tag="ACHKUC_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO6620559.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3038..4054)
                     /gene="rtcA"
                     /locus_tag="ACHKUC_004434"
     CDS             complement(3038..4054)
                     /gene="rtcA"
                     /locus_tag="ACHKUC_004434"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO6620560.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITELFFVVGEKRVSAEVVAAQLVQEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(4058..5284)
                     /gene="rtcB"
                     /locus_tag="ACHKUC_004435"
     CDS             complement(4058..5284)
                     /gene="rtcB"
                     /locus_tag="ACHKUC_004435"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO6620561.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         1287 a         1501 c         1331 g         1250 t
ORIGIN      
        1 agatcatctt tttagatggc actttcatca gaaatgaaga agaaaccctt acttaaatga
       61 aactgatgaa cataaggaaa ataccgtaca acccagtatt cacgctggat cagcgtcgtt
      121 ttaggtgagt tgttaataaa catttggaat tgtgacacag tgcaaattca gacacataaa
      181 aaaacgtcat cgcttgcatt agaaaggttt ctggccgacc ttataaccat taattacgaa
      241 gcgcaaaaaa aataatattt cctcattttc cacagtgaag tgattaacta tgctgattcc
      301 gtcaaaatta agtcgtccgg ttcgactcga ccataccgtg gttcgtgagc gcctgctggc
      361 taaactttcc ggcgcgaaca atttccggct ggcgctgatc acaagtcctg cgggctacgg
      421 aaagaccacg ctcatttccc agtgggcggc aggcaaaaac gatatcggct ggtactcgct
      481 ggatgaaggt gataaccagc aagagcgttt cgccagctat ctcattgccg ccgtgcaaca
      541 ggcaaccaac ggtcactgcg cgatatgtga gacgatggcg caaaaacggc aatatgccag
      601 cctgacgtca ctcttcgccc agcttttcat tgagctggcg gaatggcata gcccacttta
      661 tctggtcatc gatgactatc atctgatcac taatccagtg atccacgagt caatgcgctt
      721 ctttattcgc catcaaccag aaaatctcac ccttgtggtg ttgtcacgca accttccgca
      781 actgggcatt gccaatctgc gtgttcgtga ccagctgctg gaaattggca gtcagcaact
      841 ggcatttacc catcaggaag cgaagcagtt ttttgattgc cgtctgtcat cgccgattga
      901 agctgcagaa agcagtcgga tttgtgatga tgtttccggt tgggcgacgg cactgcagct
      961 aatcgccctc tccgcccggc agaatactca ctcagcccat aagtcggcac gccgcctggc
     1021 gggaatcaat gccagccatc tttcggatta tctggtcgat gaggttttgg ataacgtcga
     1081 tctcgcaacg cgccattttc tgttgaaaag cgccattttg cgctcaatga acgatgcact
     1141 catcacccgt gtgaccggcg aagaaaacgg gcaaatgcgc ctcgaagaga ttgagcgtca
     1201 ggggctgttt ttacagcgga tggatgatac cggcgagtgg ttttgctatc acccactgtt
     1261 tggtaacttc ctgcgccagc gctgccagtg ggaactggcg gcggagctgc cggaaatcca
     1321 ccgtgccgcc gcagaaagct ggatggccca gggatttccc agcgaagcga ttcatcatgc
     1381 gctggcggca ggcgatgcgc tgatgctgcg cgatattctg cttaatcacg cctggagttt
     1441 gttcaaccat agcgaactgt cgctgctgga agagtcactt aaggccctgc cgtgggacag
     1501 tttgctggaa aatccgcagt tggtgttatt gcaggcgtgg ctgatgcaaa gccagcatcg
     1561 ctacggcgaa gttaacaccc tgctggcccg cgctgaacat gaaatcaagg acatcagaga
     1621 aggcaccatg cacgcagaat ttaacgctct gcgcgcccag gtggcgatta acgatggtaa
     1681 cccggatgaa gcggaacggc tggcaaaact ggcactggaa gagctgccgc cgggctggtt
     1741 ctatagccgc attgtggcaa cctcggtgct gggtgaagtg ttgcactgca aaggcgaatt
     1801 gacccgctca ctggcgttaa tgcagcaaac cgaacagatg gcacgccagc acgatgtctg
     1861 gcactacgct ttgtggagtt taatccagca aagtgaaatt ctgtttgccc aagggttcct
     1921 gcaaaccgcg tgggaaacgc aggaaaaagc gttccagctg atcaacgagc agcatctgga
     1981 acagctgcca atgcatgagt ttctggtacg catccgtgcg cagctgttat gggcctgggc
     2041 acggctggat gaagctgaag cgtcggcgcg cagcgggatt gaagtcttgt cgtcttatca
     2101 gccacagcaa cagcttcagt gcctggcaat gttgattcaa tgctcgctgg cccgtggtga
     2161 tttagataac gcccgtagcc agctgaaccg tctggaaaac ctgctgggga atggcaaata
     2221 tcacagcgac tggatctcta acgccaacaa agtccgggtg atttactggc aaatgaccag
     2281 cgataaagcc gccgctgcca actggttgcg tcatacggct aaaccggagt ttgcgaacaa
     2341 ccacttcctg caaggtcaat ggcgcaacat tgcccgtgca caaatcttgc tgggcgagtt
     2401 tgaatctgcg gaaattgtcc tcgaagaact caatgaaaat gcccgcagtc tgcggttgat
     2461 gagcgatctc aaccgtaacc tgttgctgct taatcaactg tactggcagg ccggacgtaa
     2521 aagtgacgcc cagcgtgtgt tgctggacgc attaaaactg gcgaatcgca ccggatttat
     2581 cagtcatttt gtcatcgaag gcgaagcgat ggcgcaacaa ctgcgccagc ttattcaact
     2641 caatacgctg ccggagctgg aacagcatcg cgcacagcgt attctgcgag aaatcaatca
     2701 acatcatcgg cataaattcg cccatttcga tgagaatttc gttgaacgtc tgctaaatca
     2761 tccggaagtg ccagagctga tccgcaccag cccgttgacc caacgtgaat ggcaggtcct
     2821 ggggctgatt tactccggtt acagcaatga gcaaattgca ggcgagctgg aagtagcggc
     2881 aaccaccatc aaaacgcata tccgcaatct gtaccaaaaa ctcggtgtcg cccaccgtca
     2941 ggccgcggta caacacgcgc agaaactgct gaagatgatg ggatatggag tgtgagttta
     3001 gccggataat acgtcggatc cggcttacat ctttatatca ttcaatgctc acccgcgtta
     3061 cgccatccgc ctccaccaga ctaaaccgca ctggcaagaa acgctccacc accgcaatat
     3121 tggtcagcag atggcatgag ggatgggcga ccgtaaattc tcccgcgccc gctaacgcca
     3181 ttggcagtac cagctggtca gcgagatact cccctaccgc cgcagggctc gccaggtaac
     3241 gtttcacctc ttgcactaac tgcgcagcca ccacttcggc actgacgcgc ttttcaccga
     3301 cgacaaaaaa gagttcggtg atattttcac tttcgacttc aagcgagacg gtattacccg
     3361 gcccctggtc gcgcggcagg ttatgaatat tctgttcatg caaggaaaaa ctacccacca
     3421 gtgtagcgat ttcacgctca gcgacatggc gcggcacgcc agctaatagc acctctccac
     3481 gcatctgcac aatgttcccg cgctcgccaa gttgcaagct attaaacgat gccaccggtg
     3541 agacttccgt tgctaccacg ccgcctccgg caggataaaa accgtgacgt aacagcgtgg
     3601 tttgctgatg aactcctatt ttcgccagca gcggctccag cacccggcgg ataaaatccg
     3661 caggcggggc cgacgggtta tcggtgccgc cgctcacttc aacacgcgaa ggtccatcgg
     3721 caaaccacag cgcgggcagc accgtttgca gcaccagcgt acaacttccg gcgctaccga
     3781 tagcaaagcg gtaatcgccg ccgcgcacgg tgccaggtcg gaagaccaga cgctgcgacc
     3841 ccagctcagc gccttccacc gttgccctac aaatttccgc agccgctttt accgcggtca
     3901 gatgttggcg caacagcccc ggtttcgccc gcccggcacg aatgccggtg atggtaaatg
     3961 gctggccggt tatcatcgac aggctcagcg ccgagcgcag gatctgcccg ccgccttcgc
     4021 cctgtgcgcc atccagcgca atcatccttt tcatcattta tccttttacg cacaccacct
     4081 gacgcagggt atagataact tccaccagat cgctttgtgc cgccatcacc gcatcaatat
     4141 ctttatacgc catcgggatt tcgtcgatca cctcggcatc tttacggcac tccacatgcg
     4201 cggtggcacg gatttgatct tccacactga acagtttttt cgctttagta cggctcatta
     4261 cccgcccggc accgtggctg cacgaacaga acgactcttc atttcccagc ccacggacga
     4321 taaagctttt cgcccccatc gaaccgggaa taattccata ttgaccagca cgcgcagaca
     4381 ccgcaccttt acgcgtcaca tagatctctt cgccaaagtg ctgttctttt tgcacatagt
     4441 tgtggtgaca gttgatctct tccatcgcca gcgtttgtgg ctgttttacc gttttctgcg
     4501 taacgctctg caacgccgtt accacgtttt ccatcatcgc atcgcggtta aggctggcaa
     4561 aaagctgcgc ccaagccacg gctttcaggt aatcatcaaa gtattccgta ccttccataa
     4621 agtacgccag gtcacgcgat ggcaacgttt caagcgtttc ctgcatctct ttttgcgcca
     4681 gatcgataaa ataagttccg atggcgttac caattccgcg tgaaccggag tgcagcataa
     4741 tccacacctg gtccgactca tcaaggcaga tttcaataaa gtggttaccc gttcccagcg
     4801 ttcccaggtg tttatagttg ttagtgttaa ggaaacgggg atatttttgc gttaaccact
     4861 gataaccggc ttccagctca gcccatttcg catcgacgtt aacaggcgga ttttcccatg
     4921 cgcctttatc acgtttacaa cgcccggtag tacgtccgtg cggtacggcc gtttcaatcg
     4981 cctgacgcag ctctgccagg ttttcaggca ggtccgccgc cgttaacgcg gtacgcagtg
     5041 cgttcattcc acagccaata tccacgccca ccgccgccgg aataatcgcc cctttggtcg
     5101 ggatcacgct accaatggtg gaacctttac ccaggtgtac atcaggcatt accgcaatat
     5161 gtttgaaaat aaacggcatc ttcgcggtat taataagttg ctgacgcgca tcggcttcta
     5221 ccggcacgcc tttggtccac atttttaccg gagcattttc agtggtcagt aattcgtaat
     5281 tcattttgtt tttctctttt cgttgtttgc tgtcctgata attgcaaccg tcgtgccaga
     5341 aaatcaaata agcatcttaa ttttttaat
//
LOCUS       DAZFRG010000290         5360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFRG010000290 DAZFRG010000000
VERSION     DAZFRG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.292"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..292)
                     /locus_tag="ACHKUC_004436"
                     /pseudo
     CDS             complement(197..292)
                     /locus_tag="ACHKUC_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(289..855)
                     /gene="yecD"
                     /locus_tag="ACHKUC_004437"
     CDS             complement(289..855)
                     /gene="yecD"
                     /locus_tag="ACHKUC_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6620562.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            1165..2937
                     /gene="aspS"
                     /locus_tag="ACHKUC_004438"
     CDS             1165..2937
                     /gene="aspS"
                     /locus_tag="ACHKUC_004438"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO6620563.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2930..3382
                     /gene="nudB"
                     /locus_tag="ACHKUC_004439"
     CDS             2930..3382
                     /gene="nudB"
                     /locus_tag="ACHKUC_004439"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO6620564.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            3411..4151
                     /gene="yebC"
                     /locus_tag="ACHKUC_004440"
     CDS             3411..4151
                     /gene="yebC"
                     /locus_tag="ACHKUC_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6620565.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4186..4707
                     /gene="ruvC"
                     /locus_tag="ACHKUC_004441"
     CDS             4186..4707
                     /gene="ruvC"
                     /locus_tag="ACHKUC_004441"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO6620566.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4709..5311)
                     /gene="yebB"
                     /locus_tag="ACHKUC_004442"
     CDS             complement(4709..5311)
                     /gene="yebB"
                     /locus_tag="ACHKUC_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO6620567.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
BASE COUNT         1342 a         1305 c         1449 g         1264 t
ORIGIN      
        1 ggtgagggaa agtgattttt tggggcatgc acggggcaaa aatatttgtt gtggggcatt
       61 ttggggcatg attgggacac tttgttacat atgaactttg ccgattttca tatgacatgg
      121 caatccaacg ctttgattat tctaaagaaa acacatgctc ttgggcgttc tttagtgatt
      181 tttaaaaatc ccgtatcacg cagttaaagt ggcgggcata ctcttcaagg ctggtgatcc
      241 ccaaccgcac ccatttagga tgcgaccatt gcggcagacc gatgtaaatc ataacgcgtg
      301 gaggatctct tccacactgc gcacacgggc gatgcgcggg tagatatgat taatgctgtt
      361 attgtgctgc tcggcgctag cggcgctaca ggcatcttcg gcaatcacca gattaaaacc
      421 gagttcccag gcattgcggg cggtggattc aacaccgata ttggtcgaga tcccacataa
      481 cactattgta tcgatacccc ggcggcgtaa ttgcaactcc agatccgtac cgtaaaacgc
      541 accccattga cgtttgatga tttcgatatc gctgtcggtt gcacctaatg cagcaggatg
      601 ttgccaccag ttttcgggca acacttttgc gggggagggg gcatcaaccg gctgttttaa
      661 tgcttcggcg taatcggcag accagccaac gcgcaccaga aacacgggct gaccgctggc
      721 gcgaaatttc gccgccagct tcccggcgcg attaaccacc tcatcggcag tatgtggacc
      781 tccggcaaaa ggtaagatgc cttcttgtaa atcaatcacc accagcgcgg tggttttagc
      841 attaagttca agcatgatga ctcctgacaa aacaaaatcg tgcctctcac cttaatgcga
      901 ttagccgatg gcggaacgac aaattttgtt aatttttatg agttcgggca acacctttat
      961 agcaaactgg cgtagagtct gatttcaggc ccgatataaa gtggtaacga ggcgttccag
     1021 cgcgggccag gctcttacgg tgcggcagaa tttccagtat aatagccgcc ttttttcatt
     1081 cagttgtgac atacagctaa cgctgcgact tggtcacctg cgcagcaggg ggcatccgcc
     1141 ggcagctaag ttaagggata tctcatgcgt acagaatatt gtggacagct ccgtttgtcc
     1201 cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc gtcgtcgtga tcttggtagc
     1261 ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc aggtattttt cgatccggat
     1321 cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg agttctgcat tcaggtcacg
     1381 ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg atatggcgac tggcgaaatc
     1441 gaagtactgg cgtcctcgct gactatcatc aaccgtgcag atgttctgcc gcttgactct
     1501 aaccacgtca acaccgaaga agcgcgtctg aaataccgct acctcgacct gcgtcgcccg
     1561 gaaatggctc agcgcctgaa aacccgcgct aaaatcacca gcctggtgcg ccgttttatg
     1621 gatgaccacg gcttcctcga catcgaaact ccgatgctga ccaaagccac gcctgaaggc
     1681 gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta aattctacgc gctgccgcaa
     1741 tccccgcagt tgttcaaaca gctactgatg atgtccggct ttgaccgcta ctatcagatc
     1801 gttaaatgct tccgtgacga agacctgcgt gctgaccgtc agcctgaatt tactcagatc
     1861 gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg aagtgatgga agcgctggtg
     1921 cgtcatctgt ggctggaagt gaagggtgtg gatctgggcg atttcccggt aatgaccttt
     1981 gcagaagcag aacgccgtta tggttctgat aaaccggatc tgcgtaaccc gatggaactg
     2041 accgacgttg ccgatctgct gaaatctgtt gagtttgcag tatttgcagg tccggcgaac
     2101 gatccgaaag gccgtgttgc cgctctgcgt gttccgggcg gcgcatcgtt gacccgtaag
     2161 cagatcgacg aatacggtaa cttcgttaaa atctacggcg cgaaaggtct ggcttacatc
     2221 aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca gcccggtagc gaagttcctc
     2281 aatgcagaaa tcattgaagc catcctggag cgtactggcg cgcaagatgg cgatatgatt
     2341 ttcttcggtg ccgacaacaa gaaaattgtt gccgacgcga tgggggcact gcgcctgaaa
     2401 gtgggtaaag accttggtct gaccgacgaa agcaaatggg caccgctgtg ggttatcgac
     2461 ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg caatgcacca tccgttcacc
     2521 tcaccgaaag acatgacggc agcagaactg aaagctgcac cggaaaatgc ggtggcgaac
     2581 gcttacgata tggtcatcaa tggttacgaa gtgggcggtg gttctgtgcg tatccataat
     2641 ggtgatatgc agcagacggt gtttggtatt ctgggtatca acgaagagga acagcgcgag
     2701 aaattcggct tcctgctcga cgccctgaaa tacggtactc cgccgcacgc aggtctggca
     2761 ttcggtcttg accgtctgac gatgctgctg accggcaccg acaatatccg tgacgttatc
     2821 gccttcccga aaaccacggc ggcggcgtgt ctgatgactg aagcaccgag ctttgctaac
     2881 ccgactgcac tggctgagct gagcattcag gttgtgaaga aggctgagaa taactgatat
     2941 ggcttataag cgtcccgttt cgatcttagt ggtcatctac gcacaagata cgaaacgggt
     3001 gctgatgttg cagcggcgtg acgatcccga tttctggcag tcggtaaccg gcagcgtgga
     3061 agagggtgaa accgcgccgc aagctgccat gcgcgaagta aaggaagagg tcaccattga
     3121 tgttgtcgct gaacaactga ccttaattga ctgtcagcgc acggtagagt ttgaaatttt
     3181 ttcacattta cgtcatcgct atgcgccggg cgtgacgcgt aatacggaat catggttctg
     3241 tcttgcgctt ccgcacgagc gccagatcgt tttcactgaa catctggctt acaagtggct
     3301 tgatgcgtct gctgcggcgg cgctcactaa gtcctggagc aaccggcagg cgattgaaca
     3361 gtttgtaatt aacgctgcct gaacaggcag ggctatctgg agatattttt atggcaggtc
     3421 atagtaaatg ggccaacacc agacatcgta aagctgcgca ggatgctaag cgcggtaaaa
     3481 tcttcactaa aatcattcgt gagctggtaa ccgcggctaa gctgggcggt ggtgatccgg
     3541 acgctaaccc gcgtttgcgt gcagcaattg ataaagcact gtctaacaac atgacccgtg
     3601 acactctgaa ccgcgcaatt gcacgaggtg tgggcggtga tgatgatgca aacatggaaa
     3661 ccatcatcta cgaaggttac ggtcctggcg gcacggcaat catgattgaa tgtctgtctg
     3721 acaaccgcaa ccgtaccgtt gctgaagtgc gtcatgcatt cagcaaatgt ggcggtaacc
     3781 tcggtactga tggttccgta gcttatctgt tcagcaaaaa aggcgtgatc tccttcgaga
     3841 aaggcgatga agacaccatc atggaagcag ccctggaagc cggtgctgaa gacgttgtga
     3901 cctatgatga cggcgcgatt gatgtctaca ctgcatggga agaaatgggc aaagtacgcg
     3961 acgctctgga agcggcaggt ctgaaagcag acagcgcgga agtttccatg atcccgtcca
     4021 ccaaagctga tatggatgca gaaaccgcac cgaaactgat gcgtctgatc gatatgctgg
     4081 aagattgcga cgacgtgcag gaagtttacc ataacggtga aatctctgat gaggttgcag
     4141 cgactctctg atgaggcctg ctaaacagca aaacggagac gcgtgatggc tattattctc
     4201 ggcattgacc cgggttcgcg cgtgaccggc tatggcgtca tccgccaggt aggcaggcaa
     4261 ctgtcctacc tgggtagcgg atgcatacgc accaaagtgg atgatttacc gtctcgtctg
     4321 aagctcatct atgcgggcgt gacggaaatc atcacccaat tccagcctga ttatttcgcc
     4381 attgaacagg tctttatggc gaagaacgct gactcagccc tgaaactggg gcaggcgcgc
     4441 ggcgtggcga ttgtggcggc ggtgaatcag gaattgccgg tatttgaata tgcggcacgt
     4501 caggtaaagc aaacagtggt aggtattggt agtgccgaaa aaagccaggt gcagcatatg
     4561 gtccgcacct tgctgaaact gcccgctaat ccacaggcgg atgccgccga tgcgctggcg
     4621 attgccatca cccactgcca cgttagtcag aatgcgatgc aaatgagcga atcgcgactg
     4681 aacctggcga gagggcgact gcgttaagtt ataccgcctc ggtcagttcc ggctgaggcg
     4741 tttcaactcc caccgcgtga atcaacacca aacccggatg atgccacaaa ctggctggcg
     4801 tgacggtttt acgctcccac ggaatagaac ctaagaacca gaatttccag aaagtgagtt
     4861 ttgcattcgg attgctattt aacaattctc caaacgtctc tatttcaccc accggaatac
     4921 atagcgcctc tttataaata tcaaaaacaa attttgaaca gaactggcgc gaagattcgt
     4981 atttaaaacc ggtgtggtaa agtttgcgta gccgggaagg aacctgttca acaattcgtt
     5041 gtttttgttg ttctgttagt ccggcgtcta atcgctttat agcatagcgt tgattagcag
     5101 agcgtttaat aaaacgggat agcgtagtga tggttgagag gggaacacgg ctttctgcaa
     5161 ccagaaagtc ttcaccgtta tgaccgataa tgatcccgac gtgattactc cagcaatttg
     5221 atgcagctga tatttgacca aataaggcag cacctataca tgtaaagacg atatcaccaa
     5281 tttcatattc agcaggataa ttaatattca cgttgtcatt ccattgaaat agatatgaat
     5341 atacgtaata gcataaaaaa
//
LOCUS       DAZFRG010000291         5360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFRG010000291 DAZFRG010000000
VERSION     DAZFRG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.293"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..1544
                     /gene="cadB"
                     /locus_tag="ACHKUC_004443"
     CDS             210..1544
                     /gene="cadB"
                     /locus_tag="ACHKUC_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO6620568.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1624..3771
                     /gene="cadA"
                     /locus_tag="ACHKUC_004444"
     CDS             1624..3771
                     /gene="cadA"
                     /locus_tag="ACHKUC_004444"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO6620569.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3830..5287
                     /gene="dtpC"
                     /locus_tag="ACHKUC_004445"
     CDS             3830..5287
                     /gene="dtpC"
                     /locus_tag="ACHKUC_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO6620570.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         1259 a         1277 c         1309 g         1515 t
ORIGIN      
        1 tttatctttt catgatatca acttgcgatc ctgatgtgtt aataaaaaac ctcaagttct
       61 cacttacaga aacttttgtg ttatttcacc taatctttag gattaatcct tttttcgtga
      121 gtaatcttag cgccagtttg gtctggtcag gaaatagtta tacatcatga cccggactcc
      181 aaattcaaaa atgaaattag gagaagagca tgagttctgc caagaagatc gggctatttg
      241 cctgtaccgg tgttgttgcc ggtaatatga tggggagcgg tattgcatta ttacctgcga
      301 acctagcaag tatcggtggt attgctatct ggggttggat tatctctatt attggtgcaa
      361 tgtcgctggc atatgtatat gcccgactgg caacaaaaaa cccgcaacaa ggtggcccaa
      421 ttgcgtatgc cggagaaatt tcccctgcat ttggttttca gacaggtgtt ctttattacc
      481 atgctaactg gattggtaac ctggcaattg gtattaccgc tgtatcttat ctttccacct
      541 tcttcccagt attaaatgat cctgttccgg cgggtatcgc ctgtattgct atcgtctggg
      601 tatttacctt tgtgaatatg ctcggcggta cctgggtaag ccgtttaacc acgattggtc
      661 tggtgctggt tcttattcct gtggtgatga ctgctattgt tggctggcat tggtttgatg
      721 cagcaactta tgcagctaac tggaatactg cggataccac tgatggtcat gcgatcatta
      781 aaagtattct gctctgcctg tgggccttcg tgggtgttga atccgcagca gtaagtactg
      841 gtatggttaa aaacccgaaa cgtaccgttc cgctggcaac catgctgggt actggtttag
      901 caggtattgt ttacatcgct gcgactcagg tgctttccgg tatgtatccg tcttctgtaa
      961 tggcggcttc cggtgctccg tttgcaatca gtgcttcaac tatcctcggt aactgggctg
     1021 caccactggt ttctgcattc accgcctttg cgtgcctgac ttctctgggc tcctggatga
     1081 tgttggtagg ccaggcaggt gtacgtgccg ctaacgacgg taacttcccg aaagtttatg
     1141 gtgaagtcga cagcaacggt attccgaaaa aaggtctgct gctggctgca gtgaaaatga
     1201 ctgccctgat gatcctcatc actctgatga actctgccgg tggtaaagcc tctgacctgt
     1261 tcggtgaact gaccggtatc gcagtactgc tgactatgct gccgtacttc tactcttgcg
     1321 ttgacctgat tcgttttgaa ggcgttaaca tccgcaactt tgtcagcctg atctgctctg
     1381 tactgggttg cgtgttctgc ttcatcgcgc tgatgggcgc aagctccttc gagctggcag
     1441 gtaccttcat cgtcagcctg attatcctga tgttctacgc tcgcaaaatg cacgagcgcc
     1501 agagccactc aatggataac cacacagcgt ctaacgcaca ttaattaaaa gtattttccg
     1561 aggctcctcc tttcattttg tcccatgtgt tgggaggggc cttttttacc tggagatatg
     1621 actatgaacg ttattgcaat attgaatcac atgggggttt attttaaaga agaacccatc
     1681 cgtgaacttc atcgcgcgct tgaacgtctg aacttccaga ttgtttaccc gaacgaccgt
     1741 gacgacttat taaaactgat cgaaaacaat gcgcgtctgt gcggcgttat ttttgactgg
     1801 gataaatata atctcgagct gtgcgaagaa attagcaaaa tgaacgagaa cctgccgttg
     1861 tacgcgttcg ctaatacgta ttccactctc gatgtaagcc tgaatgacct gcgtttacag
     1921 attagcttct ttgaatatgc gctgggtgct gctgatgata ttgctaacaa gatcaagcag
     1981 accactgacg aatatatcaa cactattctg cctccgctga ccaaagcact gtttaaatat
     2041 gttcgtgaag gtaaatatac tttctgtact cctggtcaca tgggtggtac tgcattccag
     2101 aaaagcccgg taggtagcct gttctatgat ttctttggtc cgaacaccat gaaatctgat
     2161 atttccattt cagtatctga attgggttct ctgctggatc acagtggtcc acacaaagaa
     2221 gcagaacagt atatcgctcg cgtctttaac gcagaccgca gctacatggt gaccaacggt
     2281 acttccactg cgaacaaaat tgttggtatg tactctgctc cggcaggcag caccattctg
     2341 attgaccgta actgccacaa atcgctgacc cacctgatga tgatgagcga tgttacgcca
     2401 atctatttcc gcccgacccg taacgcttac ggtattcttg gtggtatccc acagagtgaa
     2461 ttccagcacg ctaccattgc taagcgcgtg aaagaaacac caaacgcaac ctggccggta
     2521 catgctgtaa ttaccaactc tacctatgat ggtctgctgt acaacaccga cttcatcaag
     2581 aaaacactgg atgtgaaatc catccacttt gactccgcgt gggtgcctta caccaacttc
     2641 tcaccgattt acgaaggtaa atgcggtatg agcggtggcc gtgtagaagg gaaagtgatt
     2701 tacgaaaccc agtctactca caaactgctg gcggcgttct ctcaggcttc catgatccac
     2761 gttaaaggtg acgtaaacga agaaaccttt aacgaagcct acatgatgca caccaccacc
     2821 tctccgcact acggtatcgt ggcgtccact gaaaccgctg cggcgatgat gaaaggcaat
     2881 gcaggtaaac gtctgatcaa tggttccatt gaacgtgcga tcaaattccg taaagagatc
     2941 aaacgtctga gaacggaatc tgatggctgg ttctttgatg tatggcagcc ggatcatatc
     3001 gatacgactg aatgctggcc gctgcgttct gacagcacct ggcacggctt caaaaacatc
     3061 gataacgagc acatgtatct tgacccgatc aaagtcaccc tgctgactcc ggggatggaa
     3121 aaagacggca ccatgagcga ctttggtatt ccggccagca tcgtggcgaa atacctcgac
     3181 gaacatggca tcgttgttga gaaaaccggt ccgtataacc tgctgttcct gttcagcatc
     3241 ggtatcgata agaccaaagc actgagcctg ctgcgtgctc tgactgactt caaacgtgcg
     3301 ttcgatctga acctgcgtgt gaaaaacatg ctgccgtctc tgtatcgtga agatcctgaa
     3361 ttctatgaaa acatgcgtat tcaggaactg gctcagaata tccacaaact gattgttcac
     3421 cacaatctgc cggatctgat gtatcgcgca tttgaagttc tgccgactat ggtaatgact
     3481 ccgtatgctg cgttccagaa agagctgcac ggtatgaccg aagaagttta cctcgacgaa
     3541 atggtaggtc gtattaacgc caatatgatc cttccgtatc cgccgggagt tcctctggta
     3601 atgccgggtg aaatgatcac cgaagaaagc cgtccggttc tggagttcct gcagatgctg
     3661 tgtgaaatcg gcgctcacta tccgggcttt gaaaccgata ttcacggtgc ataccgtcag
     3721 gctgatggcc gctataccgt taaggtattg aaagaagaaa gcaaaaaata attagctcgt
     3781 acaagggaag tggcttgcca cttccctttt ttgctcataa ggagaacaca tgaaaacacc
     3841 ctcacagccg cgcgcgatat actatatcgt ggcgatccaa atctgggagt acttcagttt
     3901 ttacggcatg cgtgccttac tcattctcta tctcacccat cagcttggtt ttgatgataa
     3961 ccatgccatc agcctgttca gcgcatatgc ttctctggtt tacgttaccc ctattctcgg
     4021 cggctggctt gccgaccgcc tgctcggcaa ccgcactgca gtgattgccg gcgcgctgtt
     4081 aatgaccctt ggccatgtgg tgctgggcat tgatacaaat tcaaccttta gcctgtatct
     4141 ggcgctggca atcattattt gtggctacgg tttattcaaa tcaaacatca gctgtttgct
     4201 tggcgagctc tacgacgaga acgatcatcg acgtgatggc ggtttttcgc tgctgtatgc
     4261 tgcgggcaat atcggttcta tcgccgcgcc catcgcctgc ggcctggctg ctcagtggta
     4321 tggatggcat gttggctttg cccttgcggg tggcggcatg tttatcggtt tgttgatttt
     4381 cttaagtggt catcgtcatt tccagtccac gcgtagtatg gataagaaag cgctcactag
     4441 cgtgaaattt gccttaccag tatggagctg gttagtggtg atgctctgtt tagccccagt
     4501 attttttacc ctgctgctgg agaacgactg gtccggatat ttgctggcga tcgtttgcct
     4561 cattgccgca caaatcattg cccgcatgat gatcaaattc cccgaacacc gccgtgctct
     4621 ttggcaaatt gtattgttga tgtttgtcgg gacgttgttc tgggtactgg cacaacaggg
     4681 cggcagtacc atcagcttgt ttatcgatcg ctttgtgaat cgtcaggcat tcaatattga
     4741 agtacctaca gcactattcc agtcggtgaa tgctattgcg gtgatgctcg ctggggttgt
     4801 tctggcctgg ctggcgtcgc cagaaagccg cggcaattca gcattgcgcg tctggctgaa
     4861 gtttgccttt ggcttactgc tgatggcttg tggctttatg ttgctggcat ttgatgcccg
     4921 acatgcagcg gctggcggtc aagcgtcaat gggcgtgatg atatccgggc tggcgctaat
     4981 gggctttgct gaactcttta ttgacccggt ggcgattgcg caaatcacgc gtctgaaaat
     5041 gtctggcgta ttaactggta tttatatgct ggcaacaggc gcagtcgcca actggctggc
     5101 aggcgtcgtg gcacagcaga cgacagagtc gcaaattagc ggtatggcaa ttgccgctta
     5161 ccagcgattc ttttctcaga tgggagagtg gacgttggct tgtgtcgcga tcatcgtggt
     5221 attggccttt gctacccgtt ttctgtttag cacgccgacg aatatgatac aggagagcaa
     5281 cgattaactg tcgtaaaaac aattggttat gaataaatta actttcagtt tatcacacta
     5341 gtaaatcgag attaataagt
//
LOCUS       DAZFRG010000292         5326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFRG010000292 DAZFRG010000000
VERSION     DAZFRG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.294"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(12..395)
                     /gene="quuD"
                     /locus_tag="ACHKUC_004446"
     CDS             complement(12..395)
                     /gene="quuD"
                     /locus_tag="ACHKUC_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO6620571.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSVAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
     gene            complement(481..621)
                     /locus_tag="ACHKUC_004447"
     CDS             complement(481..621)
                     /locus_tag="ACHKUC_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6620572.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(618..980)
                     /gene="rusA"
                     /locus_tag="ACHKUC_004448"
     CDS             complement(618..980)
                     /gene="rusA"
                     /locus_tag="ACHKUC_004448"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6620573.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYCDNVARIIKN
                     AMLDIGLAMPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(977..1267)
                     /locus_tag="ACHKUC_004449"
     CDS             complement(977..1267)
                     /locus_tag="ACHKUC_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6620574.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIEA"
     gene            complement(1260..1430)
                     /locus_tag="ACHKUC_004450"
     CDS             complement(1260..1430)
                     /locus_tag="ACHKUC_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6620575.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKCKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1430..1885)
                     /gene="ybcN"
                     /locus_tag="ACHKUC_004451"
     CDS             complement(1430..1885)
                     /gene="ybcN"
                     /locus_tag="ACHKUC_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO6620576.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1882..1983)
                     /locus_tag="ACHKUC_004452"
     CDS             complement(1882..1983)
                     /locus_tag="ACHKUC_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620577.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHPASDLCGGEA"
     gene            2333..3376
                     /locus_tag="ACHKUC_004453"
     CDS             2333..3376
                     /locus_tag="ACHKUC_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFO6620578.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            complement(3413..>5326)
                     /locus_tag="ACHKUC_004454"
     CDS             complement(3413..>5326)
                     /locus_tag="ACHKUC_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO6620579.1"
                     /translation="FTLSIKQTAATEPDSEVSAVLTAAPAEQVVGYNINLQLAVKDSQ
                     GNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYSATVTSKRAGKITIGVKSDSHDFSG
                     VKKEITFIEDRQQVTFSQFEASQNNALADGKQRNMVTVSLADRFGNVVPGYAVTLSLP
                     AGITQVGGEHAVSTDENGNAIFALISSTPGSYVITAHAGSQMSTELTVTFASNMTGAS
                     LSLTPESSSLISNIPANGKDAAVLDVQLTNTNASVNGQKIQLITSSEGLSVPTNIVTD
                     STGHVSVPLTTVKAGEYTVTARVTDGSHSVESGSVKLTFVPDVASAELTMSVSKQEIV
                     ADGSENATVNIQLVDANNNAFTGEVDLTITPSTGASLTSSNLQLDAHGQATTQFTASK
                     SGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVLEITSSASSAVVSDTNNLVFTLLLK
                     SKSGEALSGRALNVKTSGPSKYGALVVDKTTVTTDENGQATVSVHGRTVGSYKLTATL
                     NELGSDVSAVKSFSLYADDANGVLTLTKDPGYETNDGSPVGIYARFVDHFGNPLSGTV
                     EFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEFSTYESGYHWIKAKITNSKNTYEKT
                     IRTYVVKLPEKDS"
BASE COUNT         1443 a         1301 c         1127 g         1455 t
ORIGIN      
        1 acaattgcat atcaattaga attattagca acgatatcca tatctaaccg gatatctaat
       61 gccattaaca tcccttcaat tatgccctca gccttctgta accttttccc gatataacca
      121 tccgagcagc aatgcttact tgccagtgac atgaatgtca taccacatac ataataatct
      181 actaataaat cgtgtaaatc gctgttgttc tttttcagac gggccatgca cccgcaaatg
      241 atcatcgcgt catcgtcaca acattgcggg cgagatttta cttttgaagg aattaatccc
      301 ttaaaaccgg cggcaacgga cgaccaggtc acatcttcat gattattagc cgcccacgct
      361 ccccaacgct caagaaccat ctgaatatca cgcatcaact tactccacaa aaatcagacc
      421 agaacgccaa ttacaagcaa aaatcaacaa aacagtatta gttgattgtt atctctgact
      481 tcatactcct gctcctgtca gggttttggc gtaattcttc agtattcggt aatcggtcaa
      541 aacagaacca ggaaaacgat ataagcgcag gcgcacccag cggcggcgaa gacgctctgc
      601 catataagac tcaaacatca ttcatctccc agttcagtga tagtcagctc cagcttccca
      661 cctttggtaa cgggcatctt cacaacgcgg taatcaacga cctgagcatc atccagccag
      721 aaacctgctt tggtgagtgc gtcaaaagcg gccttttgca gattatcaag gtcacggcga
      781 cggcgatccg gcatgtggca ctcaatgctg attttcactg gcatagccaa gccgatatcc
      841 agcattgcgt ttttaatgat tcgggcgacg ttatcgcagt atgcctgccc ctctgcgctg
      901 acgtgcgtgc gcccacgatt atggcggtaa tagcgattat tgctcggagg ccagggtaat
      961 gtgatgctgt aggtattcac gcctcaataa ccccctcttt caaccagata acctgtgttc
     1021 tcgccatacc ttccagcgcg cattcttttg catatccagc gtcaacaaaa tgcgtgcgcc
     1081 ggtcgatttc gtcgtggcag gcagaacatg caatggtggc aatcaggtct ggcggtttga
     1141 taccggtacc gcacaatcca gccagccgga tatgtgccag tactgacgtt tcaggattgc
     1201 cattacatac gccagggatt cttacctggc attcccgacc acgcgctgct tttcttaaat
     1261 cagccatgat tcctccttgc tgccagtcgc aaccattttt tatcaaccag gctggcggta
     1321 tacccgagca gtgttggtat ttcggatggc ttcagctcag gtttacactt acgacgattt
     1381 ggtactttgt agatgtgtcc gttcatgaca cgaataagcg gtgtagccat tacgcctcct
     1441 gcttgtcgcg cagcagctgg aactcgcagc tctgcggaat agtcaggtgg cagccaatat
     1501 tcatcgccca ggcttcaacc ttacacagga agacatacat ctctccggta tcaagatcgg
     1561 aggtatggcg taacgactgg atagtagtga tttcgccggt tacgacatca accaggtcct
     1621 tggtttcata accgaggtat gtgtgtttga gagcatcttt tacccatgct gcggtagcga
     1681 acgatttccc cctgctgatg aggtattcac tgatttcgct gtaccacata tggctgagtg
     1741 cattctggga aagactgcgt ttctcacgcc acggtttaag caccatgcga aagcattttc
     1801 cgtcctccag ataaggctgg atctgctggc cgatagcgat gaagttaccg cgatgcagtt
     1861 tgatgccatc ttgtggtagg ttcacgcttc acctccacag aggtcagacg ctggatgcaa
     1921 aaaaacgcag gtgcatttct gcatctgtga agggagaaga gagtttggat tgtgtgtgcg
     1981 cataaacgtc cccgtttagc gcagaagtca ccggagttgt tcaggctccg gtgatacaat
     2041 tatggcgaat tgattattca taatcaaaca agataaggtc tcaaacttca tgcaagccaa
     2101 gatttatttc tgacagaatt atacaaagaa gctattggtc agaatctact cggactgtaa
     2161 aacatacgca taaccttaag ctctcacttt aagcattgtt gaaataatag ccgtcaagta
     2221 caaccttaac cacgactggg atatttccct ggctaccacg agttgtacgg ctattaaact
     2281 gccgttaaat tcagtaagag aatttcatcc gataagtcaa ggcatgtaaa acatgaaaat
     2341 taacaagata ttatcatctg caacactatt gtatggtatg tcaatggcca tgtcggtcgg
     2401 gagttgtgca acacctgtcc agactaatct tcctggttac accccgggtg cagatatcat
     2461 tagtgtttca ccgaccagaa accaagtcga tctcattggt gatgttgttt attcccagat
     2521 aaaaggaact cgttctgtca gacagcttca catgtcagtt cttgtcccgc gaacaaatga
     2581 tttaaaacca gccattattt attatcccgg cggcggattc atgtcttctg aacatgacaa
     2641 atttattgaa atgagaatgg ctctggcaga agctggtttt gttgtggccg ctgtagaata
     2701 cagaacaatt cctgatacat ttccagcacc agttgaggat ggaaaagctg caatacgtta
     2761 cctgagagag catgccagca attatgggat tgatcctcaa agaatcggag ttctgggtga
     2821 ctctgccggt ggatggcttg ctcagatgat gggaactaca aatggtgaca aaacctttga
     2881 taaaggtgac tttcttcagc aatcctcaga tgttcaggca gttgccacac tttatgggat
     2941 ttctgacttg ttgaatattg gcgaggggtt ccctgaatca gtgcaggagg ttcatcgctc
     3001 tcctgccgta accgaagcct taatgatcaa tggccctgca ttcagaaatt ttgcgggagc
     3061 ccccatcaca gcgtcaaaag aaaaagcgct aaacgccagt ccaatcggac atatgaaagg
     3121 agtaaaaccc ccatttctta ttatgcatgg tagcaaagac actctggttt cacctgagca
     3181 aagcgccaaa ctattcagga tgttgaagaa gaacggcgat aacgctgagt acgtgctggt
     3241 agaaggggcc gagcatggcg ataagacatg gtatcagcca attattataa acagagtcgt
     3301 tgagtggttt actaaaaacc tgggagcgcc tataaaaaca gctccccaac aacaaaaccc
     3361 aaacgctaac ctgtaaaaag agggagggct aagccctccc cattcaattt tgttaactat
     3421 ccttttcagg tagttttaca acataagtcc ttattgtttt ctcatatgta tttttgctat
     3481 tcgttatttt ggccttaatc cagtgataac cactttcata ggtgctgaat tccgaagctg
     3541 cattacctgt ccagtgtaac gttactgtag caggctccat tttgacccgt tgagagtctg
     3601 gactatcttc actaccagca gaaaactcaa ccgtaccaga taatgggttt ccaaaatgat
     3661 cgacaaatcg agcataaata cctactggag agccatcgtt tgtctcataa ccaggatctt
     3721 ttgttagagt caatactcca tttgcgtcat cagcgtacag ggagaatgat ttcaccgcgc
     3781 taacatcact gccaagttcg tttaaggtcg ccgttaattt atacgaacca accgttcgac
     3841 catgtacgct taccgtagcc tgaccgttct catcagtcgt tactgtggtt ttatcaacca
     3901 ccaacgcacc atatttagac ggcccagatg tcttaacatt caatgctcga ccacttaatg
     3961 cttcgcctga cttacttttc aacagtaacg taaataccag attgttggta tcgctcacta
     4021 cagccgaaga tgcagatgat gtgatctcca acacagcccc cttcacatcc gtcacggcat
     4081 caatattctg gcttgcggtt atgcgtttcc catccagaac atactcggcc tgaattgtgt
     4141 actggcctga tttcgatgca gtgaactgcg ttgttgcctg tccatgagca tccagttgca
     4201 gattactact ggtcaatgat gctcccgtcg atggtgtaat cgttaaatcc acctcacctg
     4261 taaacgcatt gttattcgca tcaaccaact gaatgtttac tgttgcgttt tcactgccat
     4321 cggccacaat ctcctgtttt gatacactca tagtaagttc tgcagaagca acatcgggca
     4381 caaaagttaa cttaacgctg ccagattcca cgctatggga accgtcagtc acccgtgcag
     4441 tcaccgtgta ctcaccagct ttcaccgttg taagcggaac agaaacatgc cctgttgaat
     4501 cagtcacgat atttgttggt acagataatc cttcagatga ggtgatgagc tgaatctttt
     4561 gcccattgac cgacgcattc gtatttgtca gctgcacatc taataccgct gcatccttac
     4621 cattagcagg aatattggaa attaaactac tgctttcagg cgtcagtgac agtgaagctc
     4681 cggtcatatt tgatgcaaag gtcactgtta actcagtaga catctgagaa ccagcatgag
     4741 ctgtaatcac gtaagacccc ggagtggagc ttattaacgc aaaaatagca ttaccatttt
     4801 cgtcagttga aacagcatgt tcaccaccaa cttgagttat ccctgctggt aaagacaatg
     4861 tgacagcata accaggaaca acattgccga aacggtccgc aaggcttaca gttaccatat
     4921 ttctctgttt tccatcagcc aatgcattat tttggctggc ttcaaactga gagaatgtaa
     4981 cctgctgccg gtcctcaata aaggtgattt ctttcttaac acctgagaaa tcatgactat
     5041 cagatttaac accgatagta atcttaccag ctcgttttga agttactgtc gcgctataga
     5101 caccgtcttt ttccgttaca gtcccaaact cgactccttc tgcctggtta agagcataaa
     5161 agctcagagt gttatcacca gtgattgcat ttccttgtga atccttcact gccagttgta
     5221 gattgatatt gtaaccaact acctgttctg ctggtgcagc ggttaaaaca gcactgacct
     5281 cagaatcagg ttctgttgct gctgtttgct taatactgag agtaaa
//
LOCUS       DAZFRG010000293         5286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFRG010000293 DAZFRG010000000
VERSION     DAZFRG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.295"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..1476
                     /gene="mltD"
                     /locus_tag="ACHKUC_004455"
     CDS             118..1476
                     /gene="mltD"
                     /locus_tag="ACHKUC_004455"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO6620580.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1523..2293)
                     /locus_tag="ACHKUC_004456"
     CDS             complement(1523..2293)
                     /locus_tag="ACHKUC_004456"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6620581.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2371..3171)
                     /locus_tag="ACHKUC_004457"
     CDS             complement(2371..3171)
                     /locus_tag="ACHKUC_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6620582.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3412..4326
                     /gene="yafC"
                     /locus_tag="ACHKUC_004458"
     CDS             3412..4326
                     /gene="yafC"
                     /locus_tag="ACHKUC_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO6620583.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFNAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(4323..5126)
                     /gene="dkgB"
                     /locus_tag="ACHKUC_004459"
     CDS             complement(4323..5126)
                     /gene="dkgB"
                     /locus_tag="ACHKUC_004459"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO6620584.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1380 a         1262 c         1325 g         1319 t
ORIGIN      
        1 tgaagaacgt tttgcatggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt
       61 catcggctcc gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg
      121 aaggcaaaag cgatattact cgcctctgtc ctgctcgtgg gttgccagag caccggcaac
      181 gttcaacagc acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca
      241 agtcaggcac gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct
      301 ttcattggcg acgagctaaa gatgggaatt ccggaaaacg accggattcg cgaacagaaa
      361 cagaaatatt tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat
      421 atgtactgga tagccgggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta
      481 cccatagtgg agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcgggcatc
      541 tggcagatca ttccgagcac ggggcgcaat tatggtttga aacagacacg caattatgac
      601 gcgcgtcgcg atgttgttgc ttcaacaact gccgcgctga acatgatgca gcgtctgaac
      661 aagatgtttg acggcgactg gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg
      721 gtcatgaagg caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta
      781 ccgttgccgc aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag tgatattctc
      841 aaaaacagca agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg
      901 cgtgtgcacc tgagcagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc
      961 gtcagcaagc tgaagacatt caacgctggc gtgaaaggct ccacgctggg cgcaagtggt
     1021 ccgcagtacg tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctggcttcg
     1081 ggcgaaattg ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt
     1141 gtttacaccg tacgctctgg cgacacgctt tcaagtatcg cttcacgtct cggcgttagc
     1201 accaaagatt tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagc
     1261 ttgacgattg gcgcaggtag tagcgcacag cggttggcaa acaacagcga tagcattacg
     1321 tatcgtgtgc gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc
     1381 aaagatgtga tgcgctggaa cagcgatact gcgaatctgc aaccaggcga taagctgacg
     1441 ttgtttgtga aaaacaacaa catgccagat tcctgacaaa ccagataatg aaaggcaccg
     1501 attcccccgg tgcctttttt atttatgctg ctttatgtgc ttctaccatg atgatatcac
     1561 tggtgaaaga gccatcattc tgcaaggcaa aatacgtttt cacctctgtc gatgcgctct
     1621 gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc gcgacccatg
     1681 aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcat
     1741 tgattaacct caaccactca ccgctggcgt agtttcgtac gtgagaggta tcgcgtaatg
     1801 cttctaccgt ctgtaaccag atgtcgcgca ctgggtggcc cggagacatt acgtccatca
     1861 caatcagcct accgccaggt ttcaatatcc ggttcacttc acgcagtgct gcaccaacat
     1921 catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg
     1981 gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg gcttcggcag
     2041 cttgtgccac gacatccagc atttgggcag ataagtcata cgctaccacc gcgctcacgt
     2101 tttgcgcagc aacaaagctg gcatgccctg ctccgcagcc catatcaagt acacttgcat
     2161 caggataatc agccagacgc accgccaggc gctgtaaatc tcgtccggat gcatgtacgg
     2221 tactggtcag atattcgctg gcttgagagc taaactgctt ttctacgtgg tcatggtggg
     2281 attgtgttgt cattgcgctg tccttttgtt gtttgagtat aaaagggcag cgtcaaacgt
     2341 tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg gactgaattc
     2401 aacgagtagc ggattgtgat cggaagcgcg cgtaaccagt acagaagctt cgctgacgtt
     2461 cagaccacgg tagaaaacaa aatcgagcgg gcgaccaaac gcccgacggc gctgatcatc
     2521 ggtaaaacgc acctggcgta gcgacatttc ccgcgcaaag cgatataacg cgttcatcct
     2581 tctacggctc caggcattga aatctcccgc cataatgacc gggccactgt ggtgagctat
     2641 ctgatcgcca ataggaagta actgcttact atagacatcc acgcccagac tgaagttgac
     2701 ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aagggataga ccgtcaccag
     2761 tgccgatttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc aatacactgg
     2821 atgtgccgcc gaaagggtca ttacgccaga aggatgttgt ggcagcacga aagcgggtac
     2881 ctgatcggcg gcaagatagt tagcagtcgc aaactgtact aactctggcg ttgtctgcgc
     2941 ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg acaaccattc
     3001 agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt cggtacttaa
     3061 cggttctcca ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg gtaagatcct
     3121 ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca cttttgactt
     3181 tgaaacctct atcacgtcaa cccttccgca ctcgggaggg ttctactgtt atagggattt
     3241 tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct tacaccggtc
     3301 aggattttgc tcattattga gaacattaaa tcggatggca tatctcgcat cctgatggtt
     3361 taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa aatgaaagcc
     3421 acgtcggaag aactcgccat ttttgtttca gtcgtcgaaa gcggcagctt tagccgggca
     3481 gcggaacaat tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa gctggagatg
     3541 aaacttggcg ttagcctgct taatcggacc acgcggcaac ttagcctgac ggaagaaggc
     3601 gagcgttatt ttcatcgcgt acagtcaatt ttgcaggaga tggcagcggc agaatcagaa
     3661 attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc aactccagtg
     3721 gtgctgcact ttctgatgcc gttgattaag ccttttcgtg aacgctatcc ggaagtcact
     3781 ttatcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt ggatgtcgcg
     3841 atacgcgccg gtacgttaac ggattccagc ttacgtgcca ggccgttatt taacagttat
     3901 cgaaaaatta tcgcctcccc cgattatatt tcctgctacg ggaagccaga aacgatcgac
     3961 gatttaaagc aacatatttg cctgagattc actgaacccg cttccctcaa tacctggccg
     4021 atagcctgta gcgatggaca attacatgag gtgaagtacg gtttgtcatc caatagtggg
     4081 gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc cgactacatg
     4141 atcgacaaag aaatcgctcg cggagaattg gtggagttaa tggcagataa agtgttgcca
     4201 gtggaaatgc cattcaatgc agtctattac agcgaccgtg cggtaagtac gcgcatccgg
     4261 gcttttatcg atttccttag cggacatgta aaaacagctc ccggaggagc tgtcagagag
     4321 gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt gcaatccagt
     4381 gcggcgatcg cttttttatc ttcggcatca agctgtaaat tttgtgcctt aagattactc
     4441 tccaggtttt cacgtttagt agaagaagga attactgagt aaccttcccc catagcccac
     4501 gccagaatca cttgagccgg agtcgcattg tgtttagctg caatacgagc aataacctta
     4561 tctttcagtg cattgccata cgccagagtc atataggaag taatatggat accgtgctgt
     4621 ttagcccaag cgaccacttt acggttttgc agataaggag agagttcaat ctggttagta
     4681 gcgatgtttt cagcgccaac agcagcaatc gctttttcca tcaatgggat cgtgaagttg
     4741 gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc ctgcataaac
     4801 tcttcagcag agacttcatc gcttggtgac ggccagtgga ttagcgtcag atcaacataa
     4861 tcggtacgca atttttgcag gctctctttc agactcggga tcaatttgtc tttgctgaga
     4921 ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca
     4981 atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa
     5041 tcaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagtg
     5101 cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg
     5161 aggtgattat gggcttcaga cagttgtgga aaaatggcgt aaaacgcaga ggatttttgc
     5221 gattttggca ataatagaaa tacaaacgaa aaaaaacctc gagcgttaac tcgaggcttc
     5281 ttaata
//
LOCUS       DAZFRG010000294         5207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFRG010000294 DAZFRG010000000
VERSION     DAZFRG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.296"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..1037
                     /locus_tag="ACHKUC_004460"
     CDS             186..1037
                     /locus_tag="ACHKUC_004460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6620585.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLSAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1064..2053
                     /locus_tag="ACHKUC_004461"
     CDS             1064..2053
                     /locus_tag="ACHKUC_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6620586.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGNDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2084..2977)
                     /locus_tag="ACHKUC_004462"
     CDS             complement(2084..2977)
                     /locus_tag="ACHKUC_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6620587.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3177..4103)
                     /locus_tag="ACHKUC_004463"
     CDS             complement(3177..4103)
                     /locus_tag="ACHKUC_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6620588.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRTFIDFMVEKRVKIES"
     gene            4260..5180
                     /locus_tag="ACHKUC_004464"
     CDS             4260..5180
                     /locus_tag="ACHKUC_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6620589.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         1426 a         1266 c         1183 g         1332 t
ORIGIN      
        1 tttttatgga cattcatttt tattcatcgg actgtaaatt ccggggatat cgaacttgca
       61 cgactaatct caaccctcaa acagatttat gaattttgtt cataacccct gacaaatcct
      121 gccgtctaat cgagctcttc cctttacgtt atgcttctca tgaacgataa cggaaggata
      181 acaccatgca aaaagtaaaa ctgaacaacg gtattgaaat gcccctgttg ggctttggtg
      241 tatttcagat gaccgatgcc gccgaatgcg aaagagcggt cattgacgct attaacagcg
      301 gttatcgact gattgacacc gccgcctctt atcaaaatga aatccaggtc gggaacgcac
      361 tgaaacaaag cggtatcgcc cgtaacgaac tctttgtcac cacgaaactc tggttgcagg
      421 ataccagtta cgaaggggcc aaagcccagt tcgaacgctc tctgaatcga ctgcaacttg
      481 attacgttga cctgtacttg attcaccagc catatggcga tgtccacgga gcctggcgtg
      541 ccatggaaga gctgcaacag gccggtaaaa ttcgcgctat tggcgtcagt aacttccatc
      601 ctgaccgact ggcagacctt atcgctttta ataacgtcgt tccggcggta aaccaggttg
      661 aagttaaccc tttcaatcag cagctacagg ccgttccctg gatgcaaagt cgtggcattc
      721 agccagaagc ctgggcaccg tttgcggaag ggaaaaatgg tctgttccag catcctgttc
      781 tctcggcaat tggcgagaag tatggcaaaa gcgtgggcca ggtagttctg cgctggatct
      841 accaacgagg cattgtttca ctggcaaaat cagtgcgaaa agaacgcatg gaagagaaca
      901 tcaacattct cgattttgaa ctcagccctg aagatatgct gcaaattacc gccctggata
      961 ccgcaaccag cgcgttcttc tctcaccgcg atcccgcaat ggtggaatgg ctgactggcc
     1021 gcaaacttga tgtttaagcc gcgataaaag aggtattcat tcaatgcaaa aacgttatct
     1081 gggtaaatcc ggactcgaag tctccgccct tgggctcggt tgcatgggct taagccacgg
     1141 ctacggcccg gcgaccgata cccgtcaggc tatcgaactt attcgtgctg cggttgaacg
     1201 tggcgtcacc ttcttcgata ccgccgaagt gtatggtccg tatctgaatg aagaagtggt
     1261 cggcgaagcc ttaaaaccgt ttcgtgaccg cgtggtaatt gccactaaat tcggttttac
     1321 cttcggcaat gataataagc agcagatttt aaacagccgt ccggaacata tccgtgaagc
     1381 cgttgaagga tcattacgtc gtctgaaaac agatgttatt gacctgctgt atcaacaccg
     1441 tgttgatcca gatgttccta ttgaagatgt tgcaggaacg gtgaaagacc tcatcgcaga
     1501 aggtaaggtc aaacatttcg gcctgtctga agcaggagca caaaccattc gtcgtgcaca
     1561 tgcggtacaa cctgtcaccg ccctgcaaag cgaatattcc atgtggtggc gtgagcctga
     1621 acaggaaatt ctgccgttgc ttgaggaact gggaattggt tttgtcccct tcagcccact
     1681 aggtaaaggt ttcctgacag gggcgattaa gccgggaacc actttcggca aagatgatta
     1741 ccgcagcacc gtaccgcgtt tcgcagcaca agccattgaa gccaatgaaa aactggtcac
     1801 cttgttaggt gaactggctg cggagaaagg ggtaacgtct gcacaaatcg cactggcatg
     1861 gctattagca caaaagccgt ggattgttcc catccctggc accactaaac tgcaccggct
     1921 ggaagaaaac ctggccgctg ctgacattgt tctttcgcag aaagatacgc agcagataag
     1981 cgaggcgctg gaaacaatta aaatcgtcgg tgaacgttac tcgcctgaac atcaggcacg
     2041 cgtaggccgc taatacccaa acgggtccag gataaaggac ccgttattcg gtgtagcgaa
     2101 gcgcatcgat caacaacgca aaagctggcg gatgctgctt gcggctgggg tagtaaagat
     2161 aatacccagg aaaagttgga caccactcct gtaacacctg aataagcttg cctgactcta
     2221 tatactcctg aaccatgtct tcagggacac aagcgatccc aaaccctgat aacgcggcat
     2281 caattctttc tggcaatgga ctacacgtca gttgcccatc aaccttaacg cgaagcggtt
     2341 tcccttctcg ctcgaactcc cagtggtaaa gcccaccggc agttggcaaa cgcatgttga
     2401 tgcagcgatg attttgtagc tcgtgaggcg tttcaggcgc agggtttgca gcaaaatacg
     2461 ccggagctcc caccacagcc atccgcatat ctggtccaat cctgactgca accatatctt
     2521 tatcaacatt ttcacccagt cgtatcccgg catcaaaacg gccttcaaca atatcgacaa
     2581 aaccgttatc caccaccagt tcgacattga tttcgggata ctctctgaga aaaggtttta
     2641 gcttcggcca gaccagactt cgcgcggcat gttccccggc agataaccga atattcccgg
     2701 agggcgtgcc gttcaattga ataagtgatt ccagttcctg ttcaagctcg tcaatacgcg
     2761 gttcaaggca ggcaatgatt ctctcccccg cttctgtggg agccacactg cgggtagtcc
     2821 gtgtcaggag gcggatattg agcctttcct ccagagcctt cattgcatgg ctaagtgcag
     2881 actgagagac gccgagtttg cccgctgctt tggtaaaact tcgctctctt gccaccacaa
     2941 gaaagatttg cagttcgttg aagttttctt tgagcattgg cgatttcctt gtagaagcat
     3001 cctcttcaca gacactcatg aaggggggca tagatgctat tattttaacc acattattag
     3061 taacaatgat aatctggctg tatcgaaaat aattctaaac tttcaggaga tttattatga
     3121 ataaacgtat cctcacgatt gcgccattct tcttaccgat attctgacac acatcattaa
     3181 ctttctattt ttactctttt ctccaccata aaatcaataa aagtacgaac tttagagggc
     3241 attctcaccc gtgatgaaac atatatataa aatccaggaa atgaaacggc ccaatcctca
     3301 agaatcggct gtaacttgcc cgcattgata tgttcagcga ttgcaaaatc cagatgttga
     3361 atgatacccg ttccattcag cgccattcgt atcatgcttt cgtcatcatt agaaatatac
     3421 ttacctgaca cctctatttc gaactcttca ttacccccca caacattcga aaactcccat
     3481 gctgagagtg caccgctact tttatgccgc aaccgaagac aattatggtt actcaattcc
     3541 tgaggagttt caggaatacc gtgttctttt aaataatctg gcgatgccac agtaaccagt
     3601 ttaataagcg gagaaacagg aacggcagtc atatgctcat ccagtccttg ctcaagacgt
     3661 atgcccacat cacagccgtc cgcaataatg ttagataaac cgtcatccat aatgagttct
     3721 atctttgttt tagggtaacg agtaagaaac tcgccaatat gaggctcaac aagcatcctt
     3781 gctgcaacac gggaggaatt tattctcaac agtcctgtag gttcaagttg ggtatcaccg
     3841 acatctttca acgcatcatc aatttgcccc aaagccgaca ccagcacttc atacagatgc
     3901 tgtccttcct cagtaagcga catattgcgg gtcgtacggt atatcagctt ggtgttaaga
     3961 cgttcctcaa gagattttaa attctgggac aatgcggccc gcgtaatacc catttcagag
     4021 gctgctctgg aaaaactgcc ggcacgaaca atcagcgcaa accaggcaag agaaggcaaa
     4081 attgaaggct ctattttcat cattgtaaac cttaacttaa cactgtgata gatattagac
     4141 cattgatgat aaccaataca agtcatacta ttacgccgtt cgcgatatga acacgaatgc
     4201 cagttgcgga taatagaaat gatatttaaa tctgtttagg caaatccctg gagcttgaaa
     4261 tgaaaactgt ttcgataaaa cacacttact gggatatagc tgctgatatc tatttccctg
     4321 acgattttaa tagtgaaaaa aagtatcctg ccattatcag tgcccatcca attggaagct
     4381 gcaaagaaca gacatcgggt agtgtgtatg gtgccgccct ggcaaaagct ggctttatcg
     4441 tcattgcttt tgatgccagt tttcagggaa gcagcggtgg agaaccgcgt tatctggaag
     4501 atccaacaat gcgagtaaaa gacttcagca ttgtcgttga ttaccttacc accttacctt
     4561 acgttgatgc cgggcgtatt ggtgtactgg gtatttgtgg tggaggtgga tatgccatta
     4621 atgccgcaat gacagaacgt cggattaaag ccattggtac tgtcacgggt gcaaactacg
     4681 ggcgtcttat gcgtgaggga tttacagcgt ttaatcctat cggagcactc gaagcaatgg
     4741 cgcaacaaag aacagacgaa gctaacggcg caaaacttcg cgtagatgat ttgctccctt
     4801 cttctccgga agcagctctt gaagcgggat taacagaaat agaccttttt gaagcaacag
     4861 aatattatcg ttcgccacga ggctgtgcgc ctaatggggt aaatcgttcg ctattttcac
     4921 atcagactgt tgccgtaggc tgggatgctt ttcatcttgc agaagtcctg ttaactcaac
     4981 cactaatggt tgtagtgggc gatagagtgg gcgcttttgg tgcctatcgt gacggctgcg
     5041 aaattattgg tcgtgcagcc tcaaaacata aagaactggt cgtggttgaa ggttattcac
     5101 attacgacct gtatgataag ccagaacctg tgaaacaggc gctcgataaa ttaattcctt
     5161 tttacaaaac acatttgtaa taaccttgca gggagattca ttatctc
//
LOCUS       DAZFRG010000295         5199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFRG010000295 DAZFRG010000000
VERSION     DAZFRG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.297"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(134..1315)
                     /locus_tag="ACHKUC_004465"
     CDS             complement(134..1315)
                     /locus_tag="ACHKUC_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6620590.1"
                     /translation="MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRF
                     AGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKDPSYEKQASKHALRESIACTFCT
                     LAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGK
                     LEALRKTRQLCSQIFDYAIATGRATINPASTLSSVVLHPEKQQRRMLSEEQLGAFMYQ
                     MKVSGNFMSLVTQILVLTAVRTGELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLS
                     HQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQVGWHTFTTAHGFRH
                     TFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     A"
     gene            1490..2305
                     /gene="ulaE"
                     /locus_tag="ACHKUC_004466"
     CDS             1490..2305
                     /gene="ulaE"
                     /locus_tag="ACHKUC_004466"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO6620591.1"
                     /translation="MLPAGECWLERLQLAKTLGFDFVEMSVDETDERLSRLDWSREQR
                     LALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKAIQFAQDVGIRVIQ
                     LAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYPLMNSISKALGYAH
                     YLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPGVFKNVPFGEGVVD
                     FERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMAKAGMVEAA"
     gene            2305..2991
                     /gene="ulaF"
                     /locus_tag="ACHKUC_004467"
     CDS             2305..2991
                     /gene="ulaF"
                     /locus_tag="ACHKUC_004467"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO6620592.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3120..3395)
                     /gene="yjfY"
                     /locus_tag="ACHKUC_004468"
     CDS             complement(3120..3395)
                     /gene="yjfY"
                     /locus_tag="ACHKUC_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO6620593.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            3722..4117
                     /gene="rpsF"
                     /locus_tag="ACHKUC_004469"
     CDS             3722..4117
                     /gene="rpsF"
                     /locus_tag="ACHKUC_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO6620594.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4124..4438
                     /gene="priB"
                     /locus_tag="ACHKUC_004470"
     CDS             4124..4438
                     /gene="priB"
                     /locus_tag="ACHKUC_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO6620595.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            4443..4670
                     /gene="rpsR"
                     /locus_tag="ACHKUC_004471"
     CDS             4443..4670
                     /gene="rpsR"
                     /locus_tag="ACHKUC_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO6620596.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            4712..5161
                     /gene="rplI"
                     /locus_tag="ACHKUC_004472"
     CDS             4712..5161
                     /gene="rplI"
                     /locus_tag="ACHKUC_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO6620597.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1347 a         1234 c         1352 g         1266 t
ORIGIN      
        1 ttattgtctg caggggcagt tcagaagatg tgagcaaata aaaaagaaaa cataaataaa
       61 acataaataa aaccatccgg cctccaaagc cggatggtta ttcattattt ctgcatcact
      121 gaactttcat tcatcaggcc tgctcagaaa gtgaatcaat gtagtcagcg taccattgca
      181 tcatttcacg acgaccatcg agatactgtg cgtggttgta ggtgccgcgc atgatgtttt
      241 tatcaacatg ggcaagctgg agttcaatcc aggcagagct gaaatcatgg tcatggagaa
      301 tggtactgaa agtatgccga aaaccatgag ctgtagtgaa tgtatgccag ccaacttgtt
      361 gaagaaactc gttgatgcaa ccgcgaccac gaggttgatt atctctgtat acaccaggaa
      421 taaccagctc ggagttacgg ggagctgtga ttatttttat ctctttcaat aacatttgag
      481 tctgatgaga aagtggaaca acatgcggcc ttcgcatttt cattctctct tttgggatta
      541 cccataaggc attggtaaaa tcaatttcat cccaagtagc aaggcgtaat tctcccgttc
      601 taacagcagt tagaaccagt atttgcgtta ccagagacat aaaattgcct gaaactttca
      661 tttggtacat gaaagcgcct aactgttctt cagatagcat ccgtctctgc tgtttttctg
      721 gatgaagaac aacactgctg agagtcgatg ctgggttaat agtggcgcgc cccgtcgcaa
      781 tggcgtaatc aaatatttga gaacagagct gacgagtctt acgtaacgct tcaagtttac
      841 cattcttttc tataaccctc agtgcattca atatttccaa ggggcgaata tcagccacag
      901 gacgtttccc tagcatggga ttaatatgat tctcgaatct tgcacgctgg tccgctaaat
      961 aacccgcagt ccaacgacaa gccatatgtg tgaacaactc ttctgcaaga gtacaaaatg
     1021 tgcatgcaat actttctctt agtgcatgtt tactcgcctg tttttcatat gaaggatctt
     1081 ttccttcagc gaggagttta cgtgcttcat cccgtttctg acgcgccaga gccagtgata
     1141 tacctggata gacaccaatc accaatttct tttctttacc cgcaaatcga tacttcagat
     1201 tccagtaacg agcgccagat ttcttgataa gaaggtaaag tccttgacca tcagaaagtt
     1261 tgtaatcctt gtcagcaggt ttggccgact caatttgccg aacagtaagt ggcatgatgt
     1321 taactccgaa aagtagcccc cagagtagcc cccaaaatta tattgatgat catggaggat
     1381 ggtttacgtc cagagtaaaa gaatttatga taatacctta ttttaaagtg agttaaaggg
     1441 ggtggagaga gttctgttga aatcagtgga aataaacagg gcttaataaa tcctccccgc
     1501 cggggagtgc tggctggaac gcctgcaact ggcaaaaacg ttgggcttcg attttgtcga
     1561 gatgtcggta gatgaaacgg acgagcgcct gtcgcgcctc gactggagcc gcgagcagcg
     1621 tctggcgctg gtcaatgcga ttgttgaaac cggcgtgcgc gtgccgtcca tgtgcctttc
     1681 tgctcatcgt cgtttcccgc tgggcagtga agatgacgcg gtgcgggcgc aggggctgga
     1741 gattatgcgt aaagctatcc agttcgccca ggatgtcggt attcgcgtga tccagctggc
     1801 gggctatgac gtttactatc aggaagccaa taacgaaacg cgtcgtcgtt tccgtgacgg
     1861 cctgaaagag agcgttgaga tggcaagccg cgcgcaggtg acgctggcga tggagatcat
     1921 ggattatccg ttgatgaact ccatcagcaa ggcgctgggc tacgcgcact atctcaacaa
     1981 tccgtggttc cagctctacc cggatatcgg caacctgtcg gcgtgggaca acgatgtgca
     2041 gatggagttg caggccggaa tcgggcatat cgtcgcggta catgtgaaag acaccaaacc
     2101 tggcgtcttc aaaaacgtgc cgtttggcga aggtgtagtg gatttcgaac gttgtttcga
     2161 aacgctcaaa cagagtggct attgcgggcc gtacctgatt gagatgtgga gcgaaacggc
     2221 ggaagacccg gcggcagaag tggcgaaagc gcgcgattgg gtgaaagcgc gcatggccaa
     2281 agcaggcatg gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac
     2341 atggatctgc cgcgctatgg gctggtgacc tttacctggg gcaacgtcag cgctatcgac
     2401 cgcgaacgcg ggctggtggt gatcaaaccc agcggcgttg cctacgaaac catgaaagcg
     2461 gccgatatgg tggtggttga tatgagcggc aaggtggtgg aaggggagta tcgcccatct
     2521 tccgacactg cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc
     2581 catacccact ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat cccggcgtta
     2641 ggcaccacgc acgctgacta cttctttggc gacattccgt gtacgcgcgg gttaagcgaa
     2701 gaagaggtgc agggcgagta tgaactgaac accggcaaag tgattatcga aacgctgggc
     2761 aacgccgagc cgctgcatac gccgggaatt gtggtgtatc agcacggacc gttcgcctgg
     2821 gggaaagatg cccacgatgc ggtgcataac gcggtggtga tggaagaagt ggcgaaaatg
     2881 gcgtggattg cgcgcagtat taacccacaa ctcaatcaca tcgacagctt cctgatgaat
     2941 aaacacttca tgcgtaaaca cggtcctaac gcttattacg ggcagaagta gaacacgcgc
     3001 tgcggaaatt tccttcctcg ggagataact ggtctaattc cgcagccgtt tttcaaaaaa
     3061 agccccctgc gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc
     3121 tagcgataaa tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga
     3181 attacgtggt agtacgttgc gccttgcgca tctgtctctt catttaatgc ctgacgtgct
     3241 tcactttcag tggcgaagtt atggttgata taaatcacgc ctaagctttg cacatcgtcc
     3301 atatttctgg cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa
     3361 agcacagcca gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt
     3421 gtgtgctctc ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat
     3481 ggtgaccatt tctgatgcag ttgttcgaaa aaacaccatg atgaagtgtg atgaactcca
     3541 aatcagcggt ttagaggtta attgcgaaag gggagattta tttcggctct gcccttgagt
     3601 ttagcgaggc atacaagtac tataacgcgt catttttcag ccgaccttta acacgttcct
     3661 tgcctccccg ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc
     3721 gatgcgtcat tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg
     3781 catgatcgag cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga
     3841 agactggggc cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt
     3901 tctgatgaac gttgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt
     3961 caacgatgcc gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc
     4021 tccgatggtt aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac
     4081 cgctgatgat gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt
     4141 gttgtccggc accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca
     4201 ctgccagttc gtgcttgagc atcgttctgt gcaggaggaa gccggctttc accggcaggc
     4261 atggtgtcaa atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat
     4321 aacggtcggc agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg
     4381 actgagcaaa atggttttgc atgccgagca gattgaattg atagattctg gagactagcc
     4441 atatggcacg ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag
     4501 agatcgacta taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg
     4561 tcccaagccg tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca
     4621 aacgcgctcg ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg
     4681 tccattaata cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc
     4741 aaacctgggt agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct
     4801 ggtaccgcag ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg
     4861 tcgcgctgaa ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga
     4921 gaaaatcaat gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact
     4981 gttcggttcc atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt
     5041 ggctaagagc gaagttcgtc tgccgaacgg cgttctgcgt accactggcg aacacgaagt
     5101 gagcttccag gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata
     5161 attcgttatt caacgagacg taaaaacgcc gaccattgg
//
LOCUS       DAZFRG010000296         5191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFRG010000296 DAZFRG010000000
VERSION     DAZFRG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.298"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..937)
                     /gene="glxR"
                     /locus_tag="ACHKUC_004473"
     CDS             complement(59..937)
                     /gene="glxR"
                     /locus_tag="ACHKUC_004473"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6620598.1"
                     /translation="MKLGFIGLGIMGTPMAINLARADHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLT"
     gene            complement(1037..1813)
                     /gene="hyi"
                     /locus_tag="ACHKUC_004474"
     CDS             complement(1037..1813)
                     /gene="hyi"
                     /locus_tag="ACHKUC_004474"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO6620599.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMEPYR"
     gene            complement(1826..3607)
                     /gene="gcl"
                     /locus_tag="ACHKUC_004475"
     CDS             complement(1826..3607)
                     /gene="gcl"
                     /locus_tag="ACHKUC_004475"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO6620600.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3697..4512)
                     /gene="allR"
                     /locus_tag="ACHKUC_004476"
     CDS             complement(3697..4512)
                     /gene="allR"
                     /locus_tag="ACHKUC_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO6620601.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4590..5072)
                     /gene="allA"
                     /locus_tag="ACHKUC_004477"
     CDS             complement(4590..5072)
                     /gene="allA"
                     /locus_tag="ACHKUC_004477"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO6620602.1"
                     /translation="MKLQVLPLSQEAFCAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         1326 a         1423 c         1161 g         1281 t
ORIGIN      
        1 tttttttaat attgctggcc gattcatttt ctgatatcgg ccatttttat tgcgggtatc
       61 aagtcagttt atggttagcc attaattcca gcgcctgcac taacgcagag tgatccaact
      121 ggctgccacc gtttgccgca catgtattaa acaattcctg gcaggtcgcg gtgtttggca
      181 ggttcagcgc cagtgctttc gcactttgca gtgccaggtt gagatctttc tggtgcagcg
      241 caattttgaa acctggatta aaggtgcgtt taatcatgcg ctcaccgtga acttccagaa
      301 tacgtgagga agcaaagccg cccatcagcg cctggcgcac acgtaccggg tccgcaccgg
      361 cttttgaagc aaacaacaag gcttcagaaa ccgcttcaat attgagcgcc acgataatct
      421 gatttgccac tttgcaggtt tgaccgtcgc cgttaccgcc cacgagagtg atatttttac
      481 cgagcagttc aaacagcggt tttacacgtt caaatactgc ttcatcaccg ccaaccataa
      541 tcgataacgt cccttcacgc gcgccgattt caccgccgga gactggcgca tcgagataat
      601 cgccgcccag ttcattcacc tgacgagcga aacgtttagt ttcaatcggg gaaatggagc
      661 tcatatcaac aatggttttg cccttcagcg aggctttggt acaaccattt tcaccaaaca
      721 gaacgtcttc aacctgcggt gtatccggca ccataataaa aatgatgtcc gatgcatctg
      781 ttacctggcg cgcagtttca acactaacgg cgcccagtga cagtaattca tcagcaaccg
      841 gaccaatggt tgtgacatgc agttgatgat cggcacgcgc cagattaatg gccatcggtg
      901 tacccataat gcctaagcca ataaatccca gtttcataat taacctcttt taaatttcgc
      961 ttttcctgaa ttcagacaac acgatcccct gccaacttag cggcctaaaa aagcattgtc
     1021 tgaatagcgt tacgttttaa cggtacggct ccatccagcg taaaccggct tccgtggtgg
     1081 tttggggttt atattcacag ccaacccaac cgttgtagtc agaattttca attaccgtaa
     1141 agagatattc gtaattaatt tctccggttc ccggttcgcc acgatgcgga ttatcagcaa
     1201 tttgcaggtg accaatttta tcggcccact gagtcatggt attagttaat tcaccttcca
     1261 tccgctgcat atgataaatg tcatactgaa ttttcaaatt gcagcaacca acatcgtcaa
     1321 tcagtttcag tgcctgccgg gttccggtga gatggaaacc aggaatatca aaatggttaa
     1381 taggttcaat cagtaataaa atatcttctt tcatcagcat attcgcggca taacgcaggt
     1441 tttctacaag cgttgtgtga atctgttcac tgctgaaacc agtcggcgtt ttaccgacca
     1501 gacagttaat ttttttatta cccagagcac gagcataacg aatcgctgct gctacgccat
     1561 cccgaaactc ttcttcacgg ccaggaatac aggcaatacc ccgctcgccc gccgcccagt
     1621 cacctgccgg taaattgtgc agcgtatgtt cgagtttatt actcgccagc acctgtttta
     1681 attcttcaat gtcgtagtca taagggaaca taaattcaac gccgcgaaaa ccacactgcg
     1741 cagctttctc aaaacgggcg agaaaatcat attcaccaaa taacatcgat aaattagcag
     1801 agaaacgtaa cattatttat ctcccttatt catagtgcat gaaacaggtt tcagtcggtg
     1861 catccgctgc gttatcggcg atatcttcaa attccatgac gttatccagt tcactgccca
     1921 tcgaaatatt agtcacacgc tcgagaataa cttccacgac taccggtacc cgatattgcg
     1981 ccattaagac ttttgcctgt tcaaaggctg gtgcaatgtc ttccggtttg aaaacgcgaa
     2041 ttgctttaca acctaaacct tccgctactt ttacgtggtc gacaccataa ccgttcactt
     2101 cgctggagtt aatattttcg aacgcgagct gtacgcagta atccatatca aaggcacgtt
     2161 gcgactggcg aatcagaccc agataggcgt tattgaccag tacatggatg tacggaatgt
     2221 tgaactgtgc gccaacggct aattcttcaa tcaggaactg gaagtcgaag tcaccggaaa
     2281 tcgccaccac attgcgctct ggatcagcgg cacaaacccc cagcgccgcc ggaatcgtcc
     2341 agcccagcgg gcctgcctgc ccacagttga tccagtggcg gtctttaaag acatgcagca
     2401 tttgtgccgc agcgatttgt gacaggccaa tggtggtgac ataacaaaca tcgcggccaa
     2461 aggctttgtt catctcttca tacacgcgct gcggtttcac cggcacgttg tcgaagtggg
     2521 ttttgcgcag caaagtgcgt ttgcgctgct ggcagtcggc gacccactct ttacgacacg
     2581 gcagacgacc cgctttttgc atctcctgcg ccacttcaac cagcagcgtc agcgccgctt
     2641 tagcatcaga gacaatgcca agatccggac acagtacgcg gccaatttgc gtcggctcaa
     2701 tatcgatatg aacgattttg cgcccttcgg tgtatttctc taccgaaccg gtatgacggt
     2761 tagcaaaacg gttaccgata ccaaacacca tgtcggacgc cagcaacgtt gcattaccgt
     2821 aacgatgcgc ggtttgcagg cccaccatcc cggccatcag ttcatgatcg tccgggatac
     2881 agccccagcc catcagcgtt gggatcaccg gaatgctggt cagttcagca aactgttgta
     2941 acagtacagc tgcgtcagca ttaattaccc cgcctccggc aacaatcacc ggacgttcgg
     3001 cctggattaa catttctaca gctttttcga tctgcatacg gctggcagca ggtttgtaga
     3061 ccggcagcgg ttcgtacata tcaggatcaa actcgatttc cgcaacctgt acgtcgaacg
     3121 gtaaatccac cagtaccgga cccgggcgac cagaacgcat caggtgaaac gcctgttgca
     3181 gcacacgagg caccagcgcc gcttcacgaa ctgtaaccgc cattttgctg accggtttag
     3241 caattgcttc aatatcaacg gcctggaaat cttctttatg cagacgggcg cgcggagcct
     3301 ggccggtaat acacagaata ggaatggaat cagcagaagc ggaatagagc gcggtgatca
     3361 tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc gatattccct gccgttgcgc
     3421 gggtataacc ttccgccatg tgcgaagcac cttccacatg acgcgccaga atgtgacgaa
     3481 taccgccgtg cttacgcatc gctgagtaga acggattgat tgcagctccc ggaacaccga
     3541 aggcggtggt gataccttct ttctccagca catacattgc cgcgtcaacg gctctcattt
     3601 ttgccatttt attcctacct ctattaattg gaaaaatttt ccaactctca aattttgtca
     3661 aaaaaacccg ctccgcagag cgggatacga cagacactat ggatgtgctt tcagtcccaa
     3721 cgccgtgctg atatcgcggg cggtgtctct gaccagctca ccctgactga caaaacgatc
     3781 ttctgtcagt cttgacgaag gcccggagat ggagatagcg gcaacaacac tacctacatc
     3841 atcgtaaatt gctgaagcta tgcaattcag gccgacaaca tgctcttctt tatctacggt
     3901 atagcccagt tcacgcgctt gttccaggtc cttcagcaag gtgggcatat ccacaagcgt
     3961 cgttggcgta aactgctgca aaccggtttg cagaatgatg ctcatcaact cttcttccgc
     4021 cagcggataa agcagcgcct tgcccgctcc ggaagcatgc agcggcagac gactgcccag
     4081 cggcgcacac atcctgacca tcgatttaca ctctaactga ccaattaata ccgcttcatt
     4141 gccgttacgg atcgcgacat tgaccgtttc gccggaaagt aacatcaggc ggcgcataaa
     4201 cggcccggcg acggagagta catcgcggtt atggatgtac gctgcaccga cgttaaagac
     4261 acctaatcct atatgccacc agcctaactg actgtcctga tagacaaaat ccgctgcctg
     4321 taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa tccagattga gagagatatc
     4381 gctaaccgac gaacttcccc cacttttttc cagatattgc agaatcgcaa ttccccgctc
     4441 taacgcctgt gcacccttct gtgccacagg ctccgcctgt cctggcctgc cgcgccgtct
     4501 aacttccgtc atacctacgt taactccgac ctacaaatga gcataaacaa tgaacaattc
     4561 gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac agagttcctg ttcaggaata
     4621 ctttcaacat cacagttgtc actgccgccg cgatcgatgg tcagaaaatc ggtgacgcgc
     4681 tgccaggcga aaagtgggtg atgccagacg ttacgatggt aattcactcc ctgttcgccg
     4741 ttggtaataa acgcccgcag ggttgacagg tctggtttgt cgtcacctaa cgccacgact
     4801 accacaaaca cttcaccttt catcgggata aaggcctgag tgcccaacgg atgacgttcg
     4861 agttcgtgaa tagtcagcgg cagattcgcc ggttgcgcgc ggttaatgct gataagcgta
     4921 cggtcttgct caagaatctc aaccagcgct aaatcgtgat agcgctccac caggccattg
     4981 ttaatatgga aaaaatcccg tttctgcgtt tcgattacgt cgccataagc acagaaggct
     5041 tcctgactta acggtaatac ctgaagtttc atctttttgt ccttgtaaca taattccgtt
     5101 caaccaccgc cgaccggtca gcttggtttc aataacaggc aatctaaaac cttaagagaa
     5161 caacatcaac acaaaagttg gaatattttt c
//
LOCUS       DAZFRG010000297         5181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFRG010000297 DAZFRG010000000
VERSION     DAZFRG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.299"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..585
                     /locus_tag="ACHKUC_004478"
     CDS             <1..585
                     /locus_tag="ACHKUC_004478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6620603.1"
                     /translation="ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNA
                     DRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAV
                     VHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFME
                     TLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
     gene            937..1062
                     /locus_tag="ACHKUC_004479"
                     /pseudo
     CDS             937..1062
                     /locus_tag="ACHKUC_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            1103..1178
                     /locus_tag="ACHKUC_004480"
     tRNA            1103..1178
                     /locus_tag="ACHKUC_004480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Ile,seq:cat)
     gene            1186..1262
                     /locus_tag="ACHKUC_004481"
     tRNA            1186..1262
                     /locus_tag="ACHKUC_004481"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Arg,seq:tcg)
     gene            1276..1352
                     /locus_tag="ACHKUC_004482"
     tRNA            1276..1352
                     /locus_tag="ACHKUC_004482"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1310..1312,aa:Arg,seq:tct)
     gene            1444..2403
                     /gene="stxA2b"
                     /locus_tag="ACHKUC_004483"
     CDS             1444..2403
                     /gene="stxA2b"
                     /locus_tag="ACHKUC_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO6620604.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            2416..2679
                     /gene="stxB2b"
                     /locus_tag="ACHKUC_004484"
     CDS             2416..2679
                     /gene="stxB2b"
                     /locus_tag="ACHKUC_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO6620605.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            3185..5095
                     /locus_tag="ACHKUC_004485"
     CDS             3185..5095
                     /locus_tag="ACHKUC_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6620606.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
BASE COUNT         1402 a         1107 c         1318 g         1354 t
ORIGIN      
        1 ataagctatg agcttacacc acaggaatat aacgcgattg agctattaaa tagcaataat
       61 cttatgtggg gcacttataa cctcaaaaag ttatttggcc ccgcaataag aggtaagcgt
      121 ctgaaaaacg cagatcgtat acccggctcc ttgccttttg ttactgcggg tgaaggcaat
      181 gaaggtattt ctgcatttat cagtaatgat gttgttgtat tccctgaaaa taccattacg
      241 atagatatgt ttggctccgc aaaatacaga aattatgagt atggtgcaga tgaccatgtg
      301 gcagttgttc atacagaatt ctttgacaga aatgccgtta ttttcataac agcatcaatt
      361 cataagtctt cttgtaccgg aaagttcaac tacgggagaa atttctatgc aaaagatgcc
      421 gacgagttaa tggtatcttt gccgtcaaaa gataataacc ctgattattc tttcatggag
      481 acgctaattc tagcagtaca taaactggtc atcaaagatg ttgtccagta cactgataaa
      541 aagctggtag cgtacaggca gattacatct gagaaggttg tttagtgcaa atccccacct
      601 gacagggtgg ggaatggtca ggttatcgtg ccctgatgca gaaaaacagt ttacaatcgt
      661 aaaaatcatc atatcatgac aaaagtggtt acattgccac gctgcttaac ccgccgatgc
      721 gcgggttttt tgtatccaga atcctgtaag ttatacggaa agtacacaga aaggaaggtg
      781 cgaccgcaat taataacaaa atcttaaaaa tcgcatatgg cactattagt tttctaaata
      841 ttgtatattt taagtattgc aggataaccc tgtaacgaag tttgcgtaac agcattttgc
      901 tctacgagtt tgccagcctc ccccagtggc tggctttttt atgtccgtag cgtcaaagca
      961 gcaatggcgc tggggcgtcg tgcaactggc gttgagctgg agactgaacg ttttgagcag
     1021 acggtcaggg aagttcagga tttagccagt cagaacggat gatattgcag gattagttac
     1081 gtaccgttat tatcctgcgc ccggcccttt agctcagtgg tgagagcgag cgactcataa
     1141 tcgccaggtc gctggttcaa atccagcaag ggccaccatc acataccgcc attagctcat
     1201 cgggacagag cgccagcctt cgaagctggt tgcgcggggt tcgagtcctc gatggcggtc
     1261 cattatctgt acactgcgtt gttagctcag ccggacagag caattgcctt ctaagcaatc
     1321 ggtcactggt tcgaatccag tacaacgcgc catacttatt ttttctggct cgcttttgcg
     1381 ggcctttttt atatctgcgc cgggtctggt gctgattact tcagccaaaa ggaatacctg
     1441 tatatgaagt gtatattgtt aaaatgggta ctgtgcctgt tactgggctt ttcttcggta
     1501 tcctattccc gggaatttac gatagacttt tcgacccaac aaagttatgt ctcttcgtta
     1561 aatagtatac ggacagaaat atcgacccca cttgaacata tatctcaggg gaccacatcg
     1621 gtgtctgtta ttaaccacac cccaccgggc agttattttg ctgtggatat acgagggctt
     1681 gatgtctatc aggcgcgttt tgaccatctt cgtctgatta ttgagcaaaa taatttatat
     1741 gtggccggat tcgttaatac ggcaacaaat actttctaca ggttttcaga ttttgcacat
     1801 atatcagtgc ccggtgtgac aactgtttcc atgacaacgg acagcagtta taccactctg
     1861 cagcgtgtcg cagcgctgga acgttccgga atgcaaatca gtcgtcactc actggtttca
     1921 tcatatctgg cgttaatgga gtttagtgga aatgccatga ccagagatgc atccagagca
     1981 gttctgcgtt ttgtcactgt cacagcagaa gccttacggt tcaggcaaat acagagagaa
     2041 tttcgtctgg cactgtctga aactgctcct gtttatacga tgacaccgga agaagtggac
     2101 ctcacactga actgggggag aatcagcaat gtgcttccgg agtttcgggg agagggtggt
     2161 gtcagagtgg ggcgaatatc ctttaataat atatcagcga tactgggcac agtggcggtt
     2221 atactgaatt gccatcatca gggggcgcgt tccgttcgcg ccgtgaatga agagatacaa
     2281 ccagaatgtc agataactgg cgacaggcca gttataagga taaacaatac gttatgggaa
     2341 agtaataccg cagctgcttt tctgaatcgc agggctcact ctttaaatac atccggagaa
     2401 taacaggagt taaatatgaa gaagatattt gtagcggctt tatttgcttt tgtttctgtt
     2461 aatgcaatgg cagctgattg tgcaaaaggt aaaattgagt tctctaagta taatgagaat
     2521 gatacattca cagtaaaagt ggccgggaaa gagtactgga ctaaccgctg gaatctgcaa
     2581 ccgctactgc aaagcgcaca gttaacagga atgacggtaa caatcaaatc aaatacctgt
     2641 gcgtcaggtt caggatttgc tgaagtgcag tttaattaat atcagaagca ttgctggttt
     2701 cgtggtgtgc agcaatgtag ttacagtgta atcaatgtca caattcagtc agttgaaggt
     2761 tgtctgcccg actgagaatt tgttaaaaaa atcctgcatg gtgaatcccc ctgagcggag
     2821 gggcatatca gcgtcacagg tgtttctgtt ttacctctat cctttctgtg cgggttcagg
     2881 tgctgatact gaactcaccg ggaggcaccc ggcaccatgc atgaacggta catagcgcat
     2941 acaccagccc ctctccggag gggctttctt gtgggcaaaa aaaagcccga gtgggttcgg
     3001 gcaatagcat gagatataca tttttataat cgaatggatt ttaaccagga ttcataaggc
     3061 tgcgcaactg cgcggccttt ttcgtatttc gggctgtagt cttccttctg ccattgtcct
     3121 gtaacttccg gacttcagcc cgccccttat ctgactcaca acattatccc ggccgggagg
     3181 attcatggca tttaaacact atgacgtggt cagggcggcg tcgccgtcag accttgcgga
     3241 acgaataact caaaaactga aggaagggtg gcagccttat ggtagtgcgc tgatttcgac
     3301 agctggttat ggtgcggagt tcatccagcc agttgtgagt gaggggagca tctcatcacc
     3361 agaggagcca ggcaaccgtc cgacgacctc agcgccttct gttgcgccag aatattacta
     3421 tgtgatcgcg cttgctggtc agtccaatgg tatgtcatac ggtgagggac tgccattacc
     3481 ggatacattc gacagccctg atccacgtat taaacagtta gcgcgtcgca gtacggtgac
     3541 accgggaggt gctgcatgta tctataacga tattatcccg gcagatcact gtctccatga
     3601 tgtggaggat atgacagggc ttaatcatcc caaagcggat ttatcgaaag gtcagtacgg
     3661 ctgtgtggga caggggctgc atatcgccaa aaagcttctg ccatttatac cggcgaatgc
     3721 gggcattctt cttgttccgt gctgccgtgg tggttcagct ttcaccaccg gaaccgatgg
     3781 cacatacagt gacactactg gcgcctcaga gagttccacc cgctgggggg tgggcaggcc
     3841 gctgtataag gacctcattg gtcgtacaaa agccgcgctg gcgaagaatc cgaaaaatgt
     3901 gctgcttgcc gtggtatgga tgcaagggga atttgatttt aacggaacgc cagcgaatca
     3961 tgcagcccgt tttacagaag tggtggatca ataccgtgcg gaccttgcag atatggcggg
     4021 gcagtgtgtg ggtggctcag ctgacagtgt tccctggatt tgtggagaca caacatattt
     4081 ctggaagcag aagaacgaaa cggcgtacca gacggtgtat ggtagttaca aaaataaaac
     4141 ggaaaagaat atccatttcg tgccgttcat gaccgatgag aacggggtga atgtgccgac
     4201 gaacaaaccg gaagaagacc cggatattcc ggatatcggg tattacggtt caaaatggcg
     4261 aacggacaga agcacctgga catctcagga cagggccagt catttcagtt catgggctcg
     4321 ccgtgggatt atttccgacc gtctggcaac ggcgattctg agctgcgcgg gtaagtcttc
     4381 tgcgtttgtt aatggtactg ccggggtggt tgttccagac agaccggtta ccacctcaga
     4441 gtctgtaatt ttttacgatg ccaaaaaagc ttcagacaat cagctgaaac cttatggctg
     4501 ggacggtatg gatggcagac gcacactggt tgatgacagc ggcaataaag ctctgcgaat
     4561 tgagaaaaat aacagcgcga aatcctggtc aatgtactgt gatattgctg cagacaaggc
     4621 aaaactttta ctggaaaaag gcggggaaat tgctgtccgg tttaaaatcc ccgaaaacgt
     4681 caatcttgag acaaccagaa acaagtatgc ctttggtttg tactggcgaa tagcggaatg
     4741 gccgggtgag ggtggtgaag gctatctgag ttctttcttt gtccagacag ataaagccag
     4801 tattgatgtt gcataccatc atacagttaa tcaacaaaaa gaacttggca cgtttggcgc
     4861 attcgaccat gactggcata cgcttgcatt taaatttaag ggcagtaaca gcattaatgt
     4921 tactccggtg cttgatggtg tggatggaca ggcgtttgac ctggtgaaat gggccaatac
     4981 tgctaatgga ctcaacaggt ttgtcattac ggatattaca ggtagtgcag aaacctaccc
     5041 tgtacttatt gatacggtgg aagttaaagc aaacaaagct ggagcagccg cataattgct
     5101 aaaaaaagcc gccagcggca ggaatggaag ctggcggagg taatcccaat ggagaatgta
     5161 aagaaaagat gctttcgtac a
//
LOCUS       DAZFRG010000298         5153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFRG010000298 DAZFRG010000000
VERSION     DAZFRG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.300"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..255)
                     /locus_tag="ACHKUC_004486"
     CDS             complement(<1..255)
                     /locus_tag="ACHKUC_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6620607.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVT"
     gene            complement(233..481)
                     /locus_tag="ACHKUC_004487"
     CDS             complement(233..481)
                     /locus_tag="ACHKUC_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6620608.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            complement(546..3038)
                     /locus_tag="ACHKUC_004488"
     CDS             complement(546..3038)
                     /locus_tag="ACHKUC_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6620609.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARA
                     RANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGNQTRDSV
                     NQNQQETEQNDQKAEQNSPNALQNSPNALQNEPETKQPESVAQQEVEKVCTACGQTGG
                     GNCPDCGAVMGDATYQETFDEEYQPEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNET
                     GETADHSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGP
                     EFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGE
                     FFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPF
                     EGERHNALDDARYQAKYVSAIWQKLIPNQADF"
     gene            complement(3118..3321)
                     /locus_tag="ACHKUC_004489"
     CDS             complement(3118..3321)
                     /locus_tag="ACHKUC_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6620610.1"
                     /translation="MNTLFVLILTVHLNTGESLDAITGMYNSMKECMAAAAEQKIPGN
                     CYPVDKVIHMDNNEIPAGLKTAP"
     gene            complement(3318..3506)
                     /gene="dicB"
                     /locus_tag="ACHKUC_004490"
     CDS             complement(3318..3506)
                     /gene="dicB"
                     /locus_tag="ACHKUC_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6620611.1"
                     /translation="METSLPNVNTSDGCFNIGVLLSNRDFTEDAINMRKYEPYLLNDN
                     SILSRIALLKLGIFGEWR"
     gene            complement(3517..4371)
                     /locus_tag="ACHKUC_004491"
     CDS             complement(3517..4371)
                     /locus_tag="ACHKUC_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6620612.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGRGNPKSFTAHNRAQAVFLC
                     VKHSHIRIMVGRAGQPQGWPGSVMTGISTPVRLTTLMVVENLGGELIKFIAEVAIMAT
                     ILTLSHPDVTIENGRAVTTSVAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDDAMVCTLDTFRFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            4948..>5153
                     /locus_tag="ACHKUC_004492"
     CDS             4948..>5153
                     /locus_tag="ACHKUC_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620613.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNH"
BASE COUNT         1158 a         1270 c         1231 g         1494 t
ORIGIN      
        1 ggtaacggtt gatgaaccgc cggtatcact aataattttc tcctgcagac tgagcatttg
       61 ttccatttgc tgccgggcaa gacggctgtt cgcttctgct gcaatagttt ctgccagttt
      121 ctggtcaata ctgccgagac cgtgattttt gcctgttatg ggatgcctgt aacgccagta
      181 aactttgtta tttcttttgt caaaatacgg agataatccc ggaacatcgg ttttatattt
      241 tcgcgggcgc gccatcttcc agtatcctct tcaaagcagg gtgatctgtg gcgatcacct
      301 ccggcttgtt taccattccg acaaagcgag cttgcggatc cactcgccag cgtcttccaa
      361 cttttttggg gagaggaaat atcattccgg ctttagcgta tttacttaac gtactcggag
      421 tagggaccgg ttcactgaat tcctcttttg cccactcagt gagcagaata agtcttgcca
      481 tgagcgtcgt tcgctaatca tggtcgccgc cactatagct ggtgggcaac gaccggggtt
      541 gaacattaaa aatcagcctg atttgggatc agtttttgcc agatagctga aacgtatttt
      601 gcctggtaac gggcgtcatc aagtgcatta tggcgctcac cttcgaatgg aatagccgtt
      661 ctggcatcga agtctatggc tttccccagc tcaacgattg tgcgtacatc gcgatcgttg
      721 tagtaacgcc acgggcaggg gatcccctgc cgttcgtatg aacggcgcaa aatcgtgttg
      781 tcgaagttgg ctccatttcc ccaaacctga acaaaaaatt caccggagtt ttcgtcgata
      841 aattcccgca attgtaacag tgcatcatct aacgggattt catcggtcat aatggcagat
      901 tgcgcttcgc gtgattgctt aagccaccat ttaatggtgt cccgatcaat gactccgcca
      961 gcagtttcca gatcgatagt cttactaaat tccggtccca tatctccggt ttgcggatcg
     1021 aaaaatattg cacctattga gatgatcggg gcatcaggat tttttcccat ggtttcaagg
     1081 tcgatcatta gatggtcaca cgtcctgctg gtggatgtga tttcttgatg accgttcacc
     1141 ttaattgagt gatctgccgt ctcgccagtt tcattatcgc tatcgtgatg ctgattgccg
     1201 ccagtgttct ccttgtgtgg atgttcagcg ccttccattt cctccggatc atcttcctga
     1261 acttcaggct gatactcttc atcgaatgtt tcctggtatg ttgcgtcgcc cattaccgcg
     1321 ccacaatcag ggcagttgcc gccgccggtc tgaccgcagg cggtgcagac tttttccact
     1381 tcctgttgcg ccactgattc aggctgtttc gtttctggct cgttttgtaa cgcatttggg
     1441 ctgttttgta acgcatttgg gctgttttgt tccgctttct ggtcgttctg ttccgtttct
     1501 tgctggttct ggttcacaga atcgcgggtc tggttcccct taacccattt cggatcattc
     1561 gggtcgctaa tcccttcaac aaattcacca cgtgatactg caagcagttc atcggcgtca
     1621 ggctggctga tattggctgc ctgcataatt ttgtttactt cgtcagcggt aacttttact
     1681 gaccctggtt gtgcggtcgt gtcagatgca ccagtatttt gttgtgaacc tgagtatgta
     1741 ccgtttttgc gggcgaaata ttcttctttc gtgatttcag tagccccagc agacagcgcc
     1801 ttatccagac cagaaagttt gtttgcgcga ccgtattttt cgccatcctt gtcggtgaag
     1861 aggaagtaga acggcccctc acgttctaca gatggttcga cttccacttt gcattcggtt
     1921 ttttcgttgt ccggaattgc cgtttccact gcatcagttt ctggtactgg cgacgagaga
     1981 gtatcagttg cgctctgatt tgttccttca tcttcaaaca cgccctttgt agtcaggtat
     2041 tcagtaatgt atttgttcag tgccacaggg tctttgtgaa tgtcgatcgg acgttcacgg
     2101 acaaggccaa aaatagtctg gcggtcgtag cgaagggcat caggctgttt gcgcattgat
     2161 gccgagatac gcttccagtc ttcgcggtcg ttgtcgataa cttcattttt tgcccagcga
     2221 tggatgctgc cgtcaatgtt tccggcatcc acatcaccag gccagagagc gtaggccagt
     2281 tcgtcatcca gtgttttcca tgtctgcttg tattcgcgat gaatggcagc aatgaccggg
     2341 ttgatttttc ctgttgaatt ttcagtgtgc tgttgattgg ctctggcgcg ggcgagatca
     2401 acaacagacg tgtattttcc ggcttccttg cgttcacctt cgcgacgttt tttccagatg
     2461 cgcatctctg cctgaatttc gggccatttg gcaccaggct tacatttatg cttaacccac
     2521 ccgatggcat gcagcttaag ctccggatac atggcgttaa cttctggcat tttcatcaac
     2581 gcttcaacga tatgtccgtc gaatgttgcc atgtcttcct gcaacagttc ctgcgcgcta
     2641 atcaccatat caacggtgat gttttcacat gtgtcgaact taaccatgac agcgttctgt
     2701 acttcagggg ccagcttgtc aaaagtgacg ttcatcggat ctgattcagt ctcaaccggg
     2761 acaaaggaag cagacgcctc atcccagcgg ttttcctgca tatattcagc atcccaggaa
     2821 tctagggcag ggcggggtat accgggttta tcctcgcaga caagaaattt ataagcgcag
     2881 tcctgagcag ccggataatg ttccaggaat tgccagtgaa attttgcgcg ggcgcgacgt
     2941 tcatcaccgg cttcaatggc agtggctaca gcgacggcac cttcttcctt tattgcctgt
     3001 tcgtccggaa tggcggcgca aataaagact ttactcattt tgttttacct cattacagat
     3061 ttaagggtga acaaatccct gccattgctg gcatataaaa atgaaactgg atattaatta
     3121 cggcgctgtt tttaatcctg ccgggatttc gttattgtcc atgtgaataa ctttatcgac
     3181 cggataacag ttgccgggaa ttttctgttc cgctgcggca gccatgcatt ctttcattga
     3241 gttgtacatg ccggtgattg catcaagcga ctcaccagta ttgagatgta cagtcagaat
     3301 gagtacgaat aatgtgttca tcgccactct ccgaaaatac cgagtttaag aagggcaatt
     3361 ctggagagta tggaattgtc attcagcagg tagggttcat attttctcat attgattgca
     3421 tcctcggtga aatccctgtt actgagcaga acaccaatat taaagcaccc gtcagacgta
     3481 ttaacgtttg gtagtgacgt ttccattatc gcgtcctcaa caatgaattt ttagcaattg
     3541 ctccacagtc atatttttaa ttgcgctccg gtttacaaga gtccatcctt gtttctccag
     3601 ataaaaccgg aaggtatcca gggtgcagac cattgcgtcg tcaggaacgg tttcagtgaa
     3661 tttgatattg ccgtgttcgt cgagacggat aaccagggtg cgtccgtccc cgtgaatcat
     3721 tttgtcggga gacggggcgt tattctggcg cagttctgct tccatgcggt cgaactcagc
     3781 aatgtatgcc tctttaaatg cagcggcttt tttgccagtg aagcccatca ccaggaaaac
     3841 gaagccgttt ttggtgattt ggtacattgg gagtttgcgc ccggttgagt cggtgtattc
     3901 gcttgacaca aaattgtgct cagtgaattt tgctgaacag tccagattgc gaattttatc
     3961 caacactcgt tcgtggcgtt tgccaaagaa ctcggcgatc gcaacagacg tagtgacagc
     4021 gcgaccattt tcgatggtta cgtcagggtg agaaagggta aggatagtag ccatgatggc
     4081 aacctccgcg atgaatttga ttaactcacc accgaggttt tccacgacca taagggtggt
     4141 gagacgtaca ggggtggaaa taccggtcat cacggaaccc ggccagcctt gcggctgccc
     4201 tgcacgcccc accataatgc gaatgtggct gtgcttaacg cataaaaaaa ccgcctgagc
     4261 gcggttatgc gccgtgaatg atttcgggtt tccacgcccg gcacccgttt tatgaggtgc
     4321 aggtgcacta taattccacc cgttctggtt ttcaatagct acattcaaca ttttctctac
     4381 ctttcatcac cgaagtgaac tttgttgatg cggtgcctgg tgcctccagg tgacgttaac
     4441 cagttaacaa ttaacgtcgg atacagagca ttcccgttac gcccgtaaaa gaccgcttgt
     4501 tttaactgtt ccgcgtgcgc tgagccgcat tcaccgcatc acaaaattca ctttaaaaag
     4561 ggcggcagag cagtcacgga gtagaactga taccgccaaa agtcaccaga aaattgataa
     4621 cagagggcgt tgcagcggag ttgtcactta agcgtatggt caacctgaca acccggtgtc
     4681 ctcaacgggg gaaggaataa ccccgccata cttaccgccg cgccatttcg cggattgcca
     4741 caaccggaag cgcacggtcg acgaaaattt aacgacaggc tatctatgaa ccagctacct
     4801 cgccgagcgc tttcgcgtta tggtctgact tttcagggaa atatcctttc agtaaactgt
     4861 cagtgccgga tgctcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc
     4921 ttaatcacca accctcagga gggtgaaatg tcgactgaaa atgatgaaat cattaactcc
     4981 ctgatacgcc agattaataa ttttgataaa gcattgcagc atgctgcggc gcgtagtgat
     5041 ataactcttt tagcaatttc atttcttgca tctgttatgg ataaaaatga agtcgtacga
     5101 cagagtcttg ttgattatat cgactcgctt caacccggca ctttcaatca tga
//
LOCUS       DAZFRG010000299         5139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFRG010000299 DAZFRG010000000
VERSION     DAZFRG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.301"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..1800
                     /locus_tag="ACHKUC_004493"
     CDS             4..1800
                     /locus_tag="ACHKUC_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO6620614.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFTNKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1818..2486
                     /locus_tag="ACHKUC_004494"
     CDS             1818..2486
                     /locus_tag="ACHKUC_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO6620615.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2501..3172
                     /gene="dsbI"
                     /locus_tag="ACHKUC_004495"
     CDS             2501..3172
                     /gene="dsbI"
                     /locus_tag="ACHKUC_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO6620616.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3218..4006)
                     /gene="ygiD"
                     /locus_tag="ACHKUC_004496"
     CDS             complement(3218..4006)
                     /gene="ygiD"
                     /locus_tag="ACHKUC_004496"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO6620617.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEDGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4149..4922
                     /gene="zupT"
                     /locus_tag="ACHKUC_004497"
     CDS             4149..4922
                     /gene="zupT"
                     /locus_tag="ACHKUC_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO6620618.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
     gene            complement(4980..>5139)
                     /gene="yqiD"
                     /locus_tag="ACHKUC_004498"
     CDS             complement(4980..>5139)
                     /gene="yqiD"
                     /locus_tag="ACHKUC_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO6620619.1"
                     /translation="WYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNSTR
                     EETAEKVE"
BASE COUNT         1308 a         1279 c         1267 g         1285 t
ORIGIN      
        1 tccatgtttg ataaatatag aaaaacactc gtagccggaa ctgtggcgat aaccctgggt
       61 ttgtcagcat cgggggtgat ggctgcgggt tttaaacctg cgccgcctgc cgggcaacta
      121 ggtgcggtca ttgtcgatcc ctacggcaat gcaccactga ccgctttggt tgacttagat
      181 agccatgtta tttctgacgt caaagttacc gtccatggga agggcgaaaa aggcgtagaa
      241 atcagctatc ccgtgggtca ggaatcacta aaaacttacg atggtgtacc gatttttggt
      301 ctttatcaga aatttactaa caaagtgacc gttgagtgga aagaaaacgg caaggtcatg
      361 aaagatgatt atgtggtgca cacttcggcc atcgtcaata attacatgga taaccgctct
      421 atctccgatt tacaacagac caaagttatt aaagtcgcac cgggttttga agatcgcctc
      481 tatctggtta atacccacac ctttaccgcc caaggttccg atctccactg gcatggtgag
      541 aaagataaaa atgccggtat ccttgatgcg ggtccggcaa ccggcgcact cccttttgat
      601 atcgcgccat tcacctttat cgtcgatacc gaaggtgaat accgctggtg gctggatcaa
      661 gacactttct acgatggtcg tgaccgcgat atcaacaagc gtggttatct gatgggtatc
      721 cgcgaaacgc cacgcggcac ctttaccgct gtacaaggtc agcactggta cgagttcgac
      781 atgatggggc aggtgctcga agatcacaaa ctgccgcgcg gatttgctga cgccactcat
      841 gagtccattg agacgccaaa tggcacggta ctgttgcgcg taggtaagag taactatcgt
      901 cgcgatgacg gcgtacacgt caccaccatt cgtgaccata tcctcgaagt cgataaatct
      961 ggtcgcgttg tcgatgtatg ggatctgacc aagatcctcg atccgaaacg cgatgcactg
     1021 ctcggcgcgc tggatgcagg tgcagtttgc gttaacgttg accttgccca tgcaggacaa
     1081 caggcaaaac tggaaccaga tacaccgttc ggcgacgcgc tgggtgtagg gccaggccgc
     1141 aactgggcgc acgtgaactc tatcgcctat gacgcaaaag atgactctat catcctctcc
     1201 tctcgccacc agggtgttgt gaaaattggt cgtgataagc aagtgaaatg gatccttgcg
     1261 ccctctaaag gttgggaaaa accgctggcc agcaagctgc tgaaaccggt tgatgctaac
     1321 ggtaagccaa ttacctgtaa cgaaaatggc ctgtgcgaaa actcagactt cgactttacc
     1381 tacacccagc ataccgcctg gatttccagc aaaggaacgc tcaccatttt tgataatggc
     1441 gatgggcgtc atctggaaca acctgcctta ccaaccatga aatactctcg ctttgtggaa
     1501 tataagattg atgagaagaa aggcaccgtt cagcaagtgt gggaatacgg taaagaacgt
     1561 ggctacgatt tctatagccc gattacctcc atcattgaat atcaagccga ccgtaacacc
     1621 atgtttggct tcggtggttc tattcatttg ttcgatgtcg ggcagccaac cgtcggtaag
     1681 ttgaacgaaa tcgattacaa aaccaaagaa gtgaaagtgg aaatcgacgt gctgtcagat
     1741 aaacccaatc agactcacta ccgtgcgctg ttagtccgtc cacaacagat gttcaaataa
     1801 ttaagaaggg aataaaaatg tcgaaattag ggatctcatc actatttaaa accatactgc
     1861 ttaccgcagc tttggctgtt tcatttaccg cttctgcatt taccgaaggc acggattaca
     1921 tggttctgga aaaacctatt ccgaatgccg acaaaacgct gattaaggta tttagctacg
     1981 cctgcccgtt ctgttacaag tacgacaaag cggttaccgg tccggtatcg gaaaaagtga
     2041 aagatatcgt tgctttcacg ccgttccatc tggaaaccaa aggtgaatac ggcaaacaag
     2101 caagcgaagt ctttgcggtc ttgatcaata aagataaagc agcaggcatt tccttatttg
     2161 atgctaactc tcagtttaag aaagccaagt ttgcctacta cgcggcttat cacgacaaaa
     2221 aagaacgctg gtctgacggt aaagatccgg ctgcttttat caaaaccggt ctggatgccg
     2281 caggtatgag tcaggcggat tttgaagcgg cactgaaaga accagcagtt caagaaacac
     2341 tggaaaaatg gaaagcctct tatgatgtcg ccaaaatcca gggggttcca gcctatgtcg
     2401 ttaatggtaa gtacttgatc tacaccaaga gtatcaaatc catcgacgcg atggcagacc
     2461 ttatccgtga gttagcaagc aaataacagg gaaacaggct atgggtatta agggaatgtg
     2521 gaaagatctt cgcacatcac cagttgacac actggtcaga tggcaggagc aacgcttgtt
     2581 gtggttgtta atggccgttg cgatgggcgc gttaattatc ctggcgcact ctttcttcca
     2641 gatctatctc tacatggcac cctgtgagca atgtgtatac attcgctacg ccatgtttgt
     2701 gatggttatt ggaggattgg ttgcagcgat caacccaaag aacatcatcc taaagctaat
     2761 tggctgcgtg atggcatttt acggcagcat tttgggactg aagttttcgc tcaaactgaa
     2821 cgatatccac catgcagtac ataatccgga tcccgattca ctgtttggcg tacagggttg
     2881 ctctaccgat cccacttttc cctttaacct gccgctggca cagtgggcac caaattggtt
     2941 caagcctacc ggggattgtg gctatgacgc accaatcgtt cctgatggcg tcacactgag
     3001 cagcacgcaa cagtggtttg tggaaatgta tcagcaatct gaaggctggt atctgctacc
     3061 accatggcat tttatgaata tggctcaggc ttgtatgctg gcttttggta tgtgtctggt
     3121 gctgctggtc attatgagtg gcgcatgggc gcttaaaatc atcagaggct aactcagtaa
     3181 cacccgaagc cagtcgttct ggcttcgggt tcttttatta acctatctgc accgacagca
     3241 tacttaaact gcccatttct ataccatcga ccggaatggt aattggctcc tgcccatccc
     3301 acgcccctaa gacatacaac aacggcagat agtgctctgg cgttgggttc gataacgcgc
     3361 catcttcatg gtcgagataa ttcaccagag gatgttgttc caccggccct tgccacgtca
     3421 gattcgcttt cacatactca ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt
     3481 gccacttcac tgtacgcagg ttatgtacca cgttgccact ggcgaccaac attatccctt
     3541 catcacgcag cgccgcgagt ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac
     3601 tgtcgatact caactgcacc atcgggatat cggcgtcagg gtacatctta atcagtacgc
     3661 cccacgagcc gtggtcgaag ccccaggctt ctttatccag cgtcacaggg accggcgcta
     3721 acagctcaac cagatgctgt gccagcgcag gtgaacccgg agcaggataa tgcgtatcgt
     3781 acagcgcctg cgggaagcca ccaaagtcat gaatcgtggg cggcatctcc atcgcggtca
     3841 ctcctgttcc acgggtaaac caatgagccg aaaccaccac tattgcttgc gggcgtggca
     3901 atgtcatccc caacgtctgc cagctgcggg tatacaaatt atcttccagc acgttcatcg
     3961 gactaccgtg acctaaaaac aatgctggca tacgtgttga agacatgatg atatccttaa
     4021 ctaaaggtgt cattttgata tcctcacaat acgcttgttc ggcggagtaa gaacccggat
     4081 aacaatgatg atgatcatca gttattttga cgatctgcct gaaggtgaag atttataagg
     4141 agttgtcgat gtcagtacct ctcattctga ccatactggc gggggcagcc acgtttattg
     4201 gcgcgtttct cggcgttctc gggcaaaaac cctcgaaccg cttactggcg ttttcgctag
     4261 gatttgcggc ggggatcatg ttgctcatct cattaatgga aatgcttcct gccgcattag
     4321 ccgccgaagg aatgtcgcct gtgttgggtt atggaatgtt tatcttcggt ctgcttggct
     4381 attttggcct ggaccgcatg ttgccacatg cgcatccgca ggatttaatg caaaaatcgg
     4441 tgcagccgtt gccaaaatcg atcaagcgca cagccattct gctcactctc ggcatcagtc
     4501 tgcataactt cccggaaggg attgccacct ttgtcacggc gagcagcaac ctggagctgg
     4561 gatttggcat cgccctggcc gtagcgttgc acaatattcc tgaaggtctg gcggttgcag
     4621 gcccggttta cgcggcaacg ggatctaaac gcaccgcgat tctgtgggcg ggcatttccg
     4681 ggctggcaga aattcttggt ggtgtgctgg catggttaat cctcggtagc atgatttctc
     4741 cggtggtcat ggcggcaatc atggcggcgg ttgcaggaat tatggtggcg ctctccgttg
     4801 acgaattaat gccgctcgcc aaagaaattg accccaataa taaccccagc tatggcgtac
     4861 tatgtggaat gtcagtgatg ggattcagtt tagtgctgct acaaacggtg ggaattggtt
     4921 aaaagcaaca tcagaagttg tccagcgcat ggacaacttc tgtcagcttc actaaagtct
     4981 tattctacct tttcggctgt ctcttctctc gtactgttta agtatttgtt ccgcgcttca
     5041 agcagggcgt ctctgtcctg gcgaaacgcc cgacaatatc gtttcaaatg caggaaataa
     5101 cccaccacaa ccagagcaat caatacaatc cagtaccag
//
LOCUS       DAZFRG010000300         5105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFRG010000300 DAZFRG010000000
VERSION     DAZFRG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.302"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..562
                     /gene="srlA"
                     /locus_tag="ACHKUC_004499"
                     /gene_synonym="gutA"
     CDS             2..562
                     /gene="srlA"
                     /locus_tag="ACHKUC_004499"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO6620620.1"
                     /translation="METITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALI
                     NFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAAA
                     SYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWVT
                     DLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            559..1518
                     /gene="srlE"
                     /locus_tag="ACHKUC_004500"
                     /gene_synonym="gutE"
     CDS             559..1518
                     /gene="srlE"
                     /locus_tag="ACHKUC_004500"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO6620621.1"
                     /translation="MIRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGNWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1529..1900
                     /gene="srlB"
                     /locus_tag="ACHKUC_004501"
                     /gene_synonym="gutB"
     CDS             1529..1900
                     /gene="srlB"
                     /locus_tag="ACHKUC_004501"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO6620622.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1904..2683
                     /gene="srlD"
                     /locus_tag="ACHKUC_004502"
     CDS             1904..2683
                     /gene="srlD"
                     /locus_tag="ACHKUC_004502"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO6620623.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2790..3149
                     /gene="gutM"
                     /locus_tag="ACHKUC_004503"
     CDS             2790..3149
                     /gene="gutM"
                     /locus_tag="ACHKUC_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO6620624.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3216..3989
                     /gene="srlR"
                     /locus_tag="ACHKUC_004504"
                     /gene_synonym="gutR"
     CDS             3216..3989
                     /gene="srlR"
                     /locus_tag="ACHKUC_004504"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO6620625.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            3982..4947
                     /gene="gutQ"
                     /locus_tag="ACHKUC_004505"
     CDS             3982..4947
                     /gene="gutQ"
                     /locus_tag="ACHKUC_004505"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO6620626.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4944..>5105)
                     /locus_tag="ACHKUC_004506"
     CDS             complement(4944..>5105)
                     /locus_tag="ACHKUC_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620627.1"
                     /translation="VKQNLREATEAFLRETIRQALAQNHHNWAACARMLETDVANLHR
                     LAKRLGLKD"
BASE COUNT         1151 a         1331 c         1384 g         1239 t
ORIGIN      
        1 gatagaaacc attactcatg gcgcagagtg gttcatcggg ctgttccaga aaggcggaga
       61 agtgtttacc gggatggtaa ccggcattct tccgctgtta attagcctgc tggttatcat
      121 gaacgcattg attaatttta tcggtcagca tcgtattgaa cgatttgctc aacgttgcgc
      181 cggtaaccct gtttcccgtt acctgctgct accgtgcatt ggtacgtttg tcttttgtaa
      241 cccgatgacc ttaagtcttg gtcgctttat gccggaaaaa tacaaaccca gctactacgc
      301 agcggcctct tatagctgcc actcaatgaa tggcctcttc cctcatatca accctggcga
      361 actgtttgtt tatcttggca ttgccagcgg tctgacgacg ctgaatctgc cacttggccc
      421 actggcggtg agctatctgc tggttggtct ggtcaccaac ttcttccgcg gctgggtaac
      481 cgatctgacc accgccattt tcgagaaaaa gatgggcatt caacttgaac aaaaagttcg
      541 tctggcagga gcaacatcat gatacgcatt cggatcgaaa aaggaacggg tggctggggc
      601 ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg tctatatcac cgccggtacc
      661 cgacctgcga ttgttgacaa actggcacag cttactggct ggcaagctat cgacggattt
      721 aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca
      781 ttacgctgcg gcatctatcc gaaacggcgt attcccacca ttaatatcca ctcgacgggc
      841 aagtccggcc cgctggcgca gtacattgtg gaagatattt atgtctctgg cgtaaaagaa
      901 gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct cttccgtggg ccgtgactat
      961 gacaccagca agaaaatcac cgaacaaagc gatggtttac tggcgaaggt gggaatgggt
     1021 atgggttctg ccgttgccgt gctgttccag tctggccgtg acaccatcga cactgtatta
     1081 aaaaccattc tgccgtttat ggcgttcgtc tcggcgctta tcggaatcat tatggcttct
     1141 ggccttggta actggattgc ccacggtctt gctccgctgg cgagccatcc actgggtctg
     1201 gtcatgctgg cgctcatctg ctccttcccg ctgctttcac ctttcctcgg cccaggcgca
     1261 gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg
     1321 ccgcatctgg ctttacctgc actgtttgcc atcaacgcgc aggcggcctg cgacttcatc
     1381 ccggtcggtt tgtcgctggc ggaagctcgt caggacacgg ttcgcgtcgg tgtcccttct
     1441 gttctggtga gccgcttttt aaccggcgcg ccaactgtac tgatcgcctg gtttgtctcc
     1501 ggttttatct atcaatagag gctgaaacat gaccgttatt tatcagacca ccatcacccg
     1561 tattggcgcg agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg
     1621 cgcgcctgcg gatctcgaag agtattgctt cattcattgc cacggcgagt taaaaggtgc
     1681 actccatccc ggtttgcaat tttctctcgg gcggcatcgc tatccggtga ccgccgttgg
     1741 cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt
     1801 aagcgaagcg gaatttccgg gcactgtcca tgtggcaggc cctgtacccg acgatatcgc
     1861 gccgggatcg gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc aggttgccgt
     1921 tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc cacggtctgg ctgccgaggg
     1981 gtatcgcgtg gcggttgtcg atattcagag cgacaaagcc gcaaatgtgg cacaagaaat
     2041 taacgccgaa tatggtgaag gaacagcgta cggttttggt gctgacgcca ccagcgagca
     2101 aagcgttctg gcgctttctc gtggggtaga tgaaatcttt ggtcgcgtgg atttgctggt
     2161 ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac ttccagctcg gcgattttga
     2221 ccgttcgcta caggtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttcgcgttt
     2281 gatgatccgc gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt
     2341 gggcagcaaa cacaactctg gctacagcgc cgcgaaattt ggtggcgtcg ggctgactca
     2401 atcactggcg ctggatctgg cggagtacgg cattacggtg cattcgctga tgctcggtaa
     2461 cctgctgaaa tcgcctatgt tccagtcgct attgccacaa tacgcgacca agctgggtat
     2521 caaaccggag caagtcgagc agtattacat cgataaagta ccgctcaaac gcggctgcga
     2581 ttatcaggat gtgctgaata tgctgctgtt ctacgccagt cctaaggcgt cgtactgcac
     2641 tggtcagtcg atcaatgtca ccggcggtca ggtgatgttc tgatcaacag cggagatctt
     2701 ttaaggatct ccgcgcgact aatagaatgc ctgatgcgct acgcttatca ggcctacagg
     2761 acttccgcca ctacatttaa ggagaagtta tggtatccgc actcatcacc gtcgccgtta
     2821 tcgcctggtg tgcgcaactg gccttaggcg gttggcaaat ttctcgtttt aaccgtgcct
     2881 tcgacacgct atgccagcaa gggagggttg gcgtgggccg ttccagcggg cgctttaaac
     2941 cgcgggtcgt ggtcgctatt gcgctggacg atcagcagcg catcgtcgac accttgttta
     3001 tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg
     3061 tgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
     3121 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
     3181 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
     3241 ccattctgga gtatctacaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
     3301 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
     3361 tcattcgtac ttatggcggc gtggtgttga acaaagagga atccgatccg cctatcgatc
     3421 ataaaacgct tatcaacacc cacaaaaaag agctgattgc agaagctgcc gttggtttta
     3481 ttcatgatgg cgactcaata attcttgatg ctggcagtac cgtgttgcag atggtgcccc
     3541 tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcacatc gtcaatgcgc
     3601 tatccgaact ggataacgaa caaactatac tgatgccagg cggaacattt cgcaaaaaat
     3661 cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc ttcgataaat
     3721 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccaccttt aacgaggtct
     3781 ataccgtcag taaggcgatg tgtaatgccg cgcgcgaagt gattttgatg gctgactcat
     3841 cgaagtttgg tcgtaaaagc cccaacgtgg tttgtagtct tgaaagcgtc gataagctga
     3901 ttaccgacgc aggtatcgat ccagcgtttc gtcaggcgct ggaagaaaaa gggatcgacg
     3961 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
     4021 atgctggagt tgcaggaagc aagccattta ccggaacgtc tgggcgatga tttcgttcgc
     4081 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
     4141 ggccacattg gtaagaaaat cgccgcaacg cttgccagca ccggcacacc ggcttttttt
     4201 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
     4261 ctgtttatct cttattccgg tggcgcgaag gaactggatc tcattattcc gcgtctggaa
     4321 gacaaatcta tcgctctgct ggcaatgacc ggaaaaccga cgtcaccgct gggcctggcg
     4381 gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gtccgatgca ccttgcgccg
     4441 acttccagca ccgtgaatac cctgatgatg ggcgacgcgc tggcgatggc ggtcatgcag
     4501 gcgcgcggat ttaatgaaga agattttgcc cgctcccacc cagccggggc actgggcgct
     4561 cgcttgctga acaaagtgca tcatcttatg cgtcgtgacg atgccatccc gcaggtggcg
     4621 ttaaccgcca gcgtgatgga cgcgatgctg gaacttagcc gcaccggttt aggtctggtg
     4681 gcagtgtgcg atgatcaacg actggtgaaa ggcgtcttta ccgacggtga tttacgtcgc
     4741 tggctggttg gcggcggcgc actcaccacg ccagtcaatg aagcgatgac ggtcggcggc
     4801 accacgttgc aatcgcaaag ccgtgccatc gacgccaaag aaatcctgat gaaacgcaaa
     4861 atcaccgccg caccggtagt ggatgaaaac ggcaaactca ccggcgcaat taacctgcag
     4921 gatttctatc aggccgggat tatttaatcc ttcagcccca gacgtttcgc cagccgatgc
     4981 aggttggcga cgtcggtttc cagcatccgc gcacaggcgg cccagttgtg atgattttgc
     5041 gccagtgcct gacgaatggt ttcacgcagg aacgcttctg tcgcttcacg cagattttgc
     5101 ttaac
//
LOCUS       DAZFRG010000301         5102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFRG010000301 DAZFRG010000000
VERSION     DAZFRG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.303"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..897
                     /locus_tag="ACHKUC_004507"
     CDS             <1..897
                     /locus_tag="ACHKUC_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6620628.1"
                     /translation="WRPRVQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVKFHWDRY
                     HGMTEESSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPLVMGRTYHEDNR
                     SPGSLPGTKTQMTIRSKTYKGSGFNELRFEDATDKEEIYLHAQKNMQVVVLNSKDKRV
                     NYDRAVSIGHDEFLVVANDRKVTVEGKQDHKTTKDHVSLTEGNQGLEVKGDLAQKISG
                     ALGISVQGDIVLQSDSKISLRVGGSFVVIHSGGVDIKGAKINLNGGGSPGDVILPMRP
                     VILKAAAGSGSMFVAHCPKEDK"
     gene            897..1736
                     /locus_tag="ACHKUC_004508"
     CDS             897..1736
                     /locus_tag="ACHKUC_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO6620629.1"
                     /translation="METAPVQYAIVDGAVEEGLLDFLNEVNPPHCCLYAEPIQPDLVA
                     LAPYLVEVVPEVEAWLRDKASPWGIYLTSESSMRELQQHFRRYLWVRIPEQEKPVLMR
                     FYDPRNIWVLAEVLTPRQLLFFINPVRQLSTRYGEECREDNFSSVRPAETMNIRAERP
                     SQLMLSYRQYSQLERKARDNYLDTLSVFIEENAEKEGWDDSSKAESSRILAEDYFSFC
                     QSLNIADDRSVRTMTLILLKKNIIDLRYIPDDWYELFSNQSYPGHIRVHELAQQELGF
                     IPQ"
     gene            1758..3308
                     /locus_tag="ACHKUC_004509"
     CDS             1758..3308
                     /locus_tag="ACHKUC_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620630.1"
                     /translation="MTSPSSTAGKPCVECGIPDPCIFDVISDFPEAGEKHFWSKEGMA
                     RFKLLDDGFGCDGIITITYQFDNSGTHEAWLEEESENSRKILQPFGKPNNVTLYYEGF
                     KGGMNIIDAFHSPWEYLSSITMPHDVLYEPAHYTVRIDSCYEHNSYVEIKVYPSLEIR
                     FTTGLSYEISSSQQTRAVKDRRDERRKAREAMEDTKPKDKNKLRSGWTNTTAEFELFH
                     KTSLTVELGLKVANVEYTHKYVEEIKKLRQIKQLEQMSRIDALIKSINKYFSPDPDNK
                     DGTRKYALFSAKIEPVTLGISYAYQYTDIKDGPCHFYGLYGAPFFKASFRFDIISFIC
                     AYCKMEDLVNRCREYLRKHGTSVECYIEISPEVNLDLGAVYSQKDKAWEFNTKESNIK
                     LGLKGEVSATFETNVFVVKLKSEVGASIEAKAGFGFDKRDDGMDLVLFHDGVTGAFKV
                     KVDISRGDKDRKENSEKPEKENELKWQLCKPLEVDDSPLRINLYGKENTVEKKVTPVT
                     QWQIGTDY"
     gene            3397..4362
                     /locus_tag="ACHKUC_004510"
     CDS             3397..4362
                     /locus_tag="ACHKUC_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620631.1"
                     /translation="MGTGLYSSDNHQKIRSRFNIQESYCAIKTNGVLGFSNRKDVLRE
                     NGDSTGTTSSSTNAMMLMENGENEISLEIGALRWFSDKPASTEERGHFSQKAGCSLDL
                     VRFVKQEETILSSIKVTINQQGIPEAQPDSMHPVIRKEILAEQAEPGFIDPDYFNETY
                     FPKGMKVYQFTQKVSVAGLPEWAWTRATPFTGSDEQLQKLKAAYIEMAAIISSGDRAR
                     LKASHKEALKAWSVTTGDSEDEILLSLYPKEEIEGRKARMQPIRWGDYAIRVMNNGRM
                     VQLYNKSKPTYSPLTYRFIDEDGDEVMGYYAPVFSLIDGQFIPVT"
     gene            4474..4758
                     /locus_tag="ACHKUC_004511"
     CDS             4474..4758
                     /locus_tag="ACHKUC_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO6620632.1"
                     /translation="MPLAARLNDKGTQHDGYRETVIIAASSTVFIDGLPAARQGDPLT
                     PHDKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
     gene            4777..>5102
                     /locus_tag="ACHKUC_004512"
     CDS             4777..>5102
                     /locus_tag="ACHKUC_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO6620633.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTIWQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRRNFR"
BASE COUNT         1493 a         1002 c         1347 g         1260 t
ORIGIN      
        1 tggcgtccac gggtgcagag caaaccgaag gtggacgggc cgcagagtgc gatagtgacg
       61 gggccagcgg gagaggagat tttctgcgac gaacatggtc gggtacgggt gaagtttcac
      121 tgggaccgtt atcacgggat gacggaggag agttcgtgct gggtgagggt gtcacaggcg
      181 tgggcggggc cggggttcgg taacctggcg ataccgcgtg tgggtcagga agtgatagtg
      241 gacttcctga acggggaccc ggaccagccg ctggtcatgg ggcggacgta ccatgaggac
      301 aaccgttcgc cggggagtct gccggggacg aagacgcaga tgacgatacg gtcgaagacg
      361 tataaaggaa gcggttttaa cgagctgcgc tttgaggacg ccacggacaa ggaagaaatc
      421 tacctgcacg cccagaaaaa tatgcaggtc gtggtgctga acagcaagga taagcgggtc
      481 aactatgacc gggcggtcag catcgggcat gatgaattcc tggtggtggc aaatgaccgg
      541 aaagtgacgg ttgaaggtaa acaggaccat aaaaccacca aagaccacgt cagcctgaca
      601 gagggcaatc agggactgga ggtgaaaggc gatctggcac agaaaataag cggcgcgctg
      661 ggtatcagcg tacagggcga tattgtttta cagagcgaca gcaaaatcag tctgcgggtg
      721 ggaggttcat ttgtggtgat ccattccggc ggcgtggata ttaagggcgc gaaaatcaat
      781 cttaacggcg ggggcagccc gggagacgtg atcctgccga tgcgcccggt gatcctgaaa
      841 gccgcagccg ggagtggttc gatgtttgtg gcgcactgcc cgaaggagga taagtgatgg
      901 aaacggcacc cgtacagtac gctattgtgg atggtgctgt ggaagaagga ttgctggatt
      961 ttctgaacga agtgaatccc ccacactgct gtctgtatgc agaacccata cagccggatc
     1021 tggtcgcgct ggccccctat ctcgtggaag ttgttccgga ggttgaagca tggctgagag
     1081 ataaggcgtc gccatgggga atatatctga cttctgagtc atccatgcgt gagctacagc
     1141 aacacttcag acgttatctg tgggttagga tccccgaaca ggagaaacct gttctcatgc
     1201 gattttatga tccccggaat atatgggtat tagcggaagt tcttaccccc cgccagcttc
     1261 ttttttttat caaccccgtc agacagctaa gtacccgcta tggtgaagaa tgtcgtgagg
     1321 ataatttttc ttccgtccgt cccgcagaaa ctatgaatat aagggcagaa cggccatcac
     1381 aactgatgct gagttacaga caatatagcc aactggaacg aaaggcacgg gataattatc
     1441 ttgatacatt atctgtattt atcgaagaaa atgcagaaaa agaagggtgg gatgattcat
     1501 caaaagctga atcatcccga attctggcag aagattattt ctctttttgc cagtcattaa
     1561 atatagcaga tgatcgttcc gtcagaacaa tgacgcttat cttactgaaa aaaaacataa
     1621 tcgatctccg atatattccg gatgactggt atgaactatt cagcaatcag tcatatccgg
     1681 gacatattcg ggtacatgaa cttgcacaac aggaactggg atttatcccg caataaaaag
     1741 acaaacggat agattatatg acatctccat cttcgacagc cggaaagcct tgtgtggaat
     1801 gcggcatacc tgatccatgt atttttgatg ttattagtga ttttccagag gccggcgaga
     1861 aacatttctg gtcaaaagaa ggaatggcgc gttttaagct actggacgat ggttttggat
     1921 gtgatggtat aattaccatt acctaccagt tcgacaacag tgggacacat gaggcatggt
     1981 tagaggaaga atcagaaaac agtcgtaaga tactgcaacc ttttggaaaa cccaataatg
     2041 ttacattata ttatgaaggt ttcaagggtg gaatgaatat cattgatgcg tttcattctc
     2101 cgtgggagta tctgagttca attaccatgc cacatgatgt tctttatgaa ccagcacatt
     2161 acacagtcag aattgatagc tgttacgagc acaactccta tgtcgagatt aaggtttatc
     2221 cttcattaga aattcggttt accacagggc ttagttatga gatttcgtca tcacaacaga
     2281 cacgagcggt aaaagatcga cgtgatgaac ggagaaaagc tcgggaggcg atggaagata
     2341 caaaacctaa agataaaaat aaactacgaa gcggatggac aaatacaacc gctgagtttg
     2401 agttgtttca caagacatct cttaccgttg aactgggatt aaaagtcgct aatgttgaat
     2461 atacacacaa gtatgtagag gaaataaaaa aactcagaca aataaaacaa ctggagcaga
     2521 tgagtagaat agatgcgctg attaaaagca ttaacaaata cttttcgcca gatcctgaca
     2581 acaaagatgg tacgcgtaaa tatgcgttat tcagtgcaaa aatagaacct gtcacactgg
     2641 ggatttctta tgcttatcaa tacactgata ttaaagatgg tccgtgccat ttttatggtc
     2701 tatatggtgc tccatttttc aaagcatcat tccgatttga tatcatctca tttatatgtg
     2761 catactgcaa aatggaagat ctggtaaacc gatgccgtga atatctccgc aagcatggaa
     2821 ccagtgttga atgctatatc gaaatttcac cggaagttaa tctcgatctt ggggctgtat
     2881 attctcaaaa agataaagca tgggaattta acactaaaga aagcaatatt aaacttggtt
     2941 tgaaaggtga ggttagtgca acatttgaga cgaatgtttt tgtcgtgaaa ttgaaatctg
     3001 aggttggtgc ttctatagaa gcaaaagctg ggtttggatt tgataaacga gatgatggaa
     3061 tggatcttgt tctatttcat gatggagtga cgggcgcgtt taaagttaag gttgacatat
     3121 ctcgcggtga taaagataga aaagagaata gtgagaaacc cgaaaaagaa aatgaactta
     3181 agtggcaact atgtaaacct ttggaagtag atgactcacc attacggatt aatttgtatg
     3241 gtaaggaaaa tactgtggaa aaaaaagtaa cgccagtgac gcagtggcag ataggcactg
     3301 attattgaac ggcgaagaat gaatcaaatt aaagataata aggtaattat gaaaataaaa
     3361 aatttaatat cagtcatttt actatcagga ggtattatgg ggactggatt gtactcgagc
     3421 gataaccatc aaaaaatccg cagcaggttt aatatacagg aatcatattg tgccattaag
     3481 actaatggtg tccttggatt cagcaaccga aaggatgtat tgcgagaaaa tggtgattca
     3541 accggaacca ccagttccag cactaatgcc atgatgctga tggaaaatgg tgaaaatgaa
     3601 atcagtctgg agattggggc gttaaggtgg ttttctgata aacctgccag taccgaagaa
     3661 cgagggcatt tctcccaaaa agcagggtgc agtctggatt tggttcgttt tgttaagcag
     3721 gaagaaacca tactttcttc gataaaggtg accatcaacc agcagggaat acctgaagcg
     3781 cagccagaca gcatgcatcc tgttatccga aaagagattc tggctgagca ggcagaaccc
     3841 ggatttattg atccagacta ttttaatgaa acttatttcc cgaaagggat gaaggtgtat
     3901 caatttacac aaaaggtctc ggtggcgggg cttcctgaat gggcctggac gcgagctacg
     3961 ccctttaccg gaagcgatga acagttacag aagctaaaag cagcctatat cgaaatggct
     4021 gcaattatta gcagcgggga cagggcacgt ctgaaagctt ctcacaaaga ggcactgaaa
     4081 gcctggtcag tcacaaccgg tgacagtgag gatgaaatat tgctctcgct ctatccaaaa
     4141 gaagaaatag aaggcaggaa agccagaatg cagcccatca gatggggcga ttacgctatc
     4201 cgggttatga ataatggacg tatggtgcag ctatataata aatcgaaacc aacatattct
     4261 ccgctgactt atcgtttcat agatgaagat ggtgatgaag ttatggggta ttacgctccg
     4321 gtattttcgc tgattgatgg tcagtttatc cctgtcactt gattaacctg ttgggctaca
     4381 aatcaggatg tccggtaagg tgattattgc tgagttaagc accttaccat ttttttaatt
     4441 ttttgtcgct gatattccag acaggaaaac aatatgcctt tagctgccag attaaacgac
     4501 aaaggtaccc agcatgatgg ctaccgtgag acagtcatca ttgcggcttc atcgacggta
     4561 tttattgatg gattacctgc cgcccgccag ggagatccgc tgacgccgca tgataaaccg
     4621 aagcatcccc ctcatccccg aaaaatcgcc agaggctcgt caaccgtctt tattgacggc
     4681 ctgcccgccg ccagaaccgg tgatgctatt gactgcggcg gcgtggttat tggtggcggt
     4741 acggtaaata taggctgata tgtctgagag gtatttatgt ccttaaaagg tcttcgcttt
     4801 acgctggagg ttgacggcca ggagccggac acctttgcag tggtgaattt caggttaatc
     4861 cagaaccagt cttatccgtt tgtgatgagt gtggatgtcg ccagcgattc ttttatgcag
     4921 acggcggaga tgctgctgga gaagaacgca acgctgacta tctggcaggg tgtcatcccg
     4981 ctgcgttatg tcacaggcgt ggtggcgggg tttggtatgc aggagaacaa cggctggcag
     5041 atgcgttatc acctgcgtat tgagccgccg ttatggcggt gcgggctgcg gcggaacttc
     5101 cg
//
LOCUS       DAZFRG010000302         5087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFRG010000302 DAZFRG010000000
VERSION     DAZFRG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.304"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(162..2153)
                     /gene="tkt"
                     /locus_tag="ACHKUC_004513"
     CDS             complement(162..2153)
                     /gene="tkt"
                     /locus_tag="ACHKUC_004513"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6620634.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            2431..3189
                     /gene="loiP"
                     /locus_tag="ACHKUC_004514"
                     /gene_synonym="yggG"
     CDS             2431..3189
                     /gene="loiP"
                     /locus_tag="ACHKUC_004514"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO6620635.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIHDRMSADGVK"
     gene            complement(3280..4200)
                     /gene="speB"
                     /locus_tag="ACHKUC_004515"
     CDS             complement(3280..4200)
                     /gene="speB"
                     /locus_tag="ACHKUC_004515"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO6620636.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4336..5067)
                     /locus_tag="ACHKUC_004516"
     CDS             complement(4336..5067)
                     /locus_tag="ACHKUC_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620637.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1217 a         1378 c         1343 g         1149 t
ORIGIN      
        1 gctacgctta tcaggcctac atacttcctg caatatgttg aatttgcaaa atcttgtagg
       61 acggataagg cgttcacgcc gcatccggca taaacaacgc gcactttgcc aacaatctga
      121 agggccgcat ttgcggccct tctcacaaaa catcttaccg attacagcag cgcttttgct
      181 ttcgcaacaa cgttgtcaac ggtgaagccg aactcttcaa acagctgctc tgccggagca
      241 gattcaccga aggtggtcat gccgacgata gcaccgttca ggccaacata cttgtaccag
      301 tagtcagcaa tgccagcttc caccgcaaca cgagcggtaa ccgctttcgg cagtacggat
      361 tcacggtaag ccgcgtcctg cttgtcgaat gcgtcggtag acggcatgga aaccacgcgc
      421 gctttcacgc cttcggcagt cagtttttcg taggcggcaa cagccagttc aacttcagaa
      481 ccggtcgcga tgaagatcag ttccggctga ccagcacagt ctttcagcac ataaccaccg
      541 cgcgcgatgt ttgccagctg ctcttcagta cgttcctgct gcgccaggtt ctgacgggag
      601 aggataagcg cagtcgggcc gtcctgacgc tcaacgccgt atttccacgc tactgcggat
      661 tcaacctggt cacacggacg ccatgtagac atgttcgggg tcacgcgcag agaagcgatc
      721 tgttctaccg gctggtgagt cgggccatct tcgcccagac cgatggagtc gtgggtgtaa
      781 accatcacct gacgctgttt catcagcgca gccatacgta cggcattacg tgcgtattcc
      841 acgaacatca ggaaggttga ggtatacggc aggaaaccac cgtgcaggga gataccgtta
      901 gcaatcgcgg tcataccgaa ctcgcgaaca ccgtagtgga tgtagttacc tgcagcatct
      961 tcgttgattg ctttagaacc agaccacagg gtcaggttag acggagcaag gtcagcggag
     1021 ccgccgagga attccggcaa cagcggaccg aacgcttcga tagcattctg agacgcttta
     1081 cggctggcga ttttcgccgg attagcctga agtttagcaa tgaactcttt cgctttcgcg
     1141 tcgaagtcag acggcatttc gcctttcata cggcgggtaa attcagcggc ttcctgcgga
     1201 taagctttcg cgtaagcagc gaatttctcg ttccatgcgg actctttcgc ctggcctgct
     1261 tctttcgcat cccactgagc atagatttca gacgggattt cgaacggcgc gtatttccag
     1321 cccagctgtt cgcgggtcag ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg
     1381 tgggtaccgg ctttgttcgg ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac
     1441 ggtttgtcag tcactgcgcg cgcttcttct actgcgcgtt tgatggatgc cgcgtcatga
     1501 ccgtcgatat cgcgaataac gtgccagccg taagcttcga aacgcattgc ggtgtcgtcg
     1561 gtaaaccagc cttcaacgtg accatcgata gaaataccgt tgtcatcgta gaacgcaatc
     1621 agtttaccca gcttcagcgt acccgccaga gagcaaactt cgtgggagat gccttccatc
     1681 atgcagccgt cgcccatgaa ggcgtaggtg tagtggtcga cgatgtcgtg gcccggacgg
     1741 ttaaactgcg ccgccagcgt tttttctgca atcgccatac cgactgcgtt ggcaataccc
     1801 tgacccagcg gaccggtggt ggtttctaca cctgcggtgt aacccacttc cgggtgaccc
     1861 ggagttttag agtgcagctg acggaagttt ttcagttctt ccatcggcag atcgtaaccg
     1921 gtgaggtgca gcaggctgta gatcagcatg gagccgtggc cgttggacag cacgaagcgg
     1981 tcacggtcag cccaggacgg attctgcggg ttgtgtttca ggaaatcacg ccacaggact
     2041 tcggcaatgt cagccatacc cataggggca cccgggtgac cggatttggc tttctgtact
     2101 gcgtccatgc tcagcgcacg aatagcattg gcaagctctt tacgtgagga cattttgact
     2161 ccagatcgga tgatgaaggg cacgccctta acgacttgac gacagcgcgt tttgggctac
     2221 gccggaaaat ttgccaacaa tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt
     2281 tgccgcttca gaaatctctg gatcatgctc gcatgttgcg caatccactc gcccgtccgc
     2341 tgcgcttttc cttatactga gactgagcgt cgattcacct gcaaacggcg catttttaga
     2401 ataatcctga ccttgtgcgg aagagaaaac atgaaaattc gcgccttatt ggtagcaatg
     2461 agcgtggcta cggtgctgac cggttgccag aatatggact ccagtggact gctctcatca
     2521 ggagcggaag cttttcaggc ttacagtttg agtgatgcgc aggtgaaaaa actgagcgat
     2581 caggcatgcc aggagatgga cagcaaagcg acgattgcgc cagccaatag cgaatacgct
     2641 aaacgtctga caactattgc caatgcgcta ggcaacaata tcaacggtca gccggtgaat
     2701 tacaaagtgt atatggcgaa ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc
     2761 gtctatagcg ggctgatgga tatgatgacg gataacgaag tcgaagcggt gctcggtcac
     2821 gaaatggggc acgtggcatt aggccatgtg aagaaaggaa tgcaggtagc acttggtaca
     2881 aacgcaattc gcgttgcggc ggcctctgca ggcggaattg tcggcagttt gtcacaatca
     2941 caactgggag acctgggcga aaagctagtc aattcgcaat ttacccaacg tcaggaatca
     3001 gaggccgatg attactccta cgacttgctg cgccaacgcg gtatcagccc ggcaggtctt
     3061 gccaccagct ttgaaaaact ggcgaaactg gaagaaggcc gccaaagctc aatgtttgac
     3121 gaccatcctg catccgccga acgcgcccag catattcacg atcgtatgag cgcggatgga
     3181 gttaagtaaa gccaggtagt gccgaacgta ggtcggataa ggcgttcacg ccgcatccga
     3241 cattaatgac acatcacccg tgcgcctctt atctggcgct tactcgccct ttttcgccgc
     3301 ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg
     3361 atcgtatgcc ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaagcc
     3421 gcgtaccagt ttaatggcgc gatcggaggt caggccgcca attaccggcg taccggtgcc
     3481 tggtgcaaaa gcaggatcca ggcagtcgat atcaaaggtc aggtaaaccg gcatatcacc
     3541 cacaatctgt ttcacctggg cgataatgtc atccacgctg cgatcgttca cctggcaggc
     3601 gtccagcacg gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga
     3661 atgattcggg tcgatcagac cttctttcgg cgcggtatag aacatggtgc cgtggtcaaa
     3721 ttcacaaccg ttagcgtagg tatcggtgtg ggcatcaaag tgtaccaacg ccattttgcc
     3781 gaaatgcttc gcatgagcgc gcagcagcgg cagcgtaaca aagtggtcac caccgaagga
     3841 gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tctcgctcat
     3901 ctcacgggca tcgccgaagg catataccag atcaccgcag tccacgacgt tcaggcgctc
     3961 acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg
     4021 acggatagct gccggaccgt ggcgtccacc ggcacgacca gaagtggcca tatcgaacgg
     4081 cacgccagta atcacccagt cagcatcgct gtcatacggc tggaagttca tcggcaggcg
     4141 taaaaaacca aaggcgttgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat
     4201 ggatggactc ctattacaaa gtcgatataa aaaaacccct ccgcgtcgtt aggcccgacg
     4261 aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccctaa atgggcaagg
     4321 catcaagcaa ggttactatt tccgacacgg tggcggcgca tagccattgt tatctgccgg
     4381 aaacgcttgt ccagagcacg gcggcagcgc gttttgcgcc gggccttgta attcaaaggc
     4441 ggcaaagccg attacaccgg tattacgcac atcgccttgc gccgaattgg ctgcgtagga
     4501 atcagcaacg ttggcgaact gaaaagccgc ttcggtatgt ttatctttgc ggaatccttt
     4561 aatcgctaac gaatctccgg cattcacaat ataaccgttg ttattcagcg aaccttgctt
     4621 gccgttcagc acatccaggc catcaaccgt agcaacgatc tcgtagttgg tgttacgact
     4681 gtaattacgg acatataatt gataattcat tccgactctg gcatggacaa tatagcgtgg
     4741 ttcattattg gatgaatcat aaacccgcgt aataggtaga gaattaaaat taccatcgcg
     4801 aatagcatat tccagttcgt taatacgaat gctataaacg cggtcgtagc cggaaggaac
     4861 attagtcgaa taactaataa cgatcatctc tgccggatcg cgccaggctc gttcagcaga
     4921 tacgccctga accgttgagt gcacatcccc accccaggcg gtgccgacat tgcctgatgc
     4981 actgctatta tactgcgcat tactggaaca accggccagc agcacggcta atgctatcaa
     5041 cgcgctacgt actttccatt ttttcataac cgttccctct gcattta
//
LOCUS       DAZFRG010000303         5050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFRG010000303 DAZFRG010000000
VERSION     DAZFRG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.305"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(183..339)
                     /locus_tag="ACHKUC_004517"
                     /pseudo
     CDS             complement(183..339)
                     /locus_tag="ACHKUC_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(539..964)
                     /locus_tag="ACHKUC_004518"
     CDS             complement(539..964)
                     /locus_tag="ACHKUC_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6620638.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1043..1906)
                     /locus_tag="ACHKUC_004519"
     CDS             complement(1043..1906)
                     /locus_tag="ACHKUC_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620639.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1906..2874)
                     /locus_tag="ACHKUC_004520"
     CDS             complement(1906..2874)
                     /locus_tag="ACHKUC_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO6620640.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2876..4534)
                     /locus_tag="ACHKUC_004521"
     CDS             complement(2876..4534)
                     /locus_tag="ACHKUC_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO6620641.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4568..4936)
                     /locus_tag="ACHKUC_004522"
     CDS             complement(4568..4936)
                     /locus_tag="ACHKUC_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620642.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT         1201 a         1398 c         1106 g         1345 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagcactcg cacacaaaaa
       61 attaccgcac gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcggga gattactccc ccgcctggtc
      181 tcttacttct cagattcgta gtctacgaag acagcgacct ccgtctggcc ggttcggatc
      241 cgtacctcgc agaggtcttt cctcgttacc agtgccgtca ctatgacagt taaacagatg
      301 acgatcaggg cgaattaaca tcgccttttg ctgcttcata gactgcttct ccttgccttc
      361 cggcacgtaa gaggctaacc tacggttgtg tgacatagat tgggcctcag attaatgtta
      421 agcgtcttgc aggacacgta atgttaactg gggcttttct ccgtctgcct tacggcagca
      481 tgcccgaggc agacaccctc aagcacccgc agtcattcta cagtaaaacc ccgcgctttc
      541 aatttcccgt atgtccggaa aactgactga cctgtgcccc tgatcgttca cgctcaacgt
      601 atctgtccat ttccagttct attccctgca taatcaacat cccctcaata acaccttcag
      661 ctttttgcag tacccgcccg gcccagcaat cagagcgctc atgcttacgg cccagtccca
      721 taagcgtcat cccgaacaca taataatcat acagaaaatc atgcaaatcg ctgttgttga
      781 cgttcagttg cgccatgcaa ttgctgataa tcagcgcatc atcatcggta cactgaggcc
      841 gtgatttaac ctttgatggg atcactccct taaatccggc agcgacgccg gaccatgcca
      901 cactctcatg attatttacc acccatgcac cccatcggga cagaacctgt tggatatcac
      961 gcattattct gtactccgct gtaaccatac tttttctgat ttgaatgctc agggggaaaa
     1021 tgtctactgg atatctccgg cttcattgct cacactcccc aaccagattg agaatgaccg
     1081 ctgaaccatt gtcttccatg tactcatctt ttccgcttgc cagaaaccag cgacacactt
     1141 caaccgcttc atcacgcgtc acaggcttta tttttcccag caaattttcg agccagaact
     1201 gccgatcata tactgactca tgatgttcac ctgtgaaacc gtatttgtcc cctgtttctc
     1261 tccccgctgt ctggcgcatc tcataaagcc agtcccagta aataaactca cgaacaacgt
     1321 ctgacaatgt atgaggctct ggcagtacat cacgatagcc atcaacatat gcacgacgct
     1381 gatcatcaat ttcatttgag cgaacaccgt caatacttcc ggcttttttc tctgccgcag
     1441 tccagcccca gtagtaatcg tcgataaatt tcggggaggt gcgaattatc cgctcagcct
     1501 ccacatcgtc gagtgctgcc tcatagctgc caaatgtggc cctgactgat gaggattttt
     1561 tgatattctc acgggcattc ctgatcgcct gcgccgggtt atccatgcct atggttgcga
     1621 aagcaatctg gaaaggatca acattaatct cctgcaaata acgtccatac cgttcactcg
     1681 cttcttccgg cgttattttg agtttctcca gtgctgcttc ggctgcgtcc agatgtacgg
     1741 cctcgttcag gtgaataacc tccagaatcc agagataagc atcagtctgt ttatgtccgg
     1801 tgatcctcct ttgttctggc agaggtttga tatttgccgg aacggcgctg tacgctgccg
     1861 tcgggatagt gaataaagct ttatgtacct gattgtccgt acgcatcacg caaccgcctt
     1921 ttttctgtaa aaaaccagtt cccgaacctg atcgccgttc atgagcatat tgttgaaatc
     1981 atcatgatca ggccaccgga cacttacccg ctgaatgtca tttcgggcct ggagatttgc
     2041 atgtgcgcat tcacaggccg cagccattcc ggtagcactg tattcatcgc gatcggcaaa
     2101 aataatcaga tgggtgactc ccgccggtac gcggaacttt ttcataaaac cactgttaat
     2161 ggttgcccag gtatttactt tgtaaatctg atgtgcggac aacgcggttt cgataccttc
     2221 agctataccc agtgtactgg ctaccggaaa catcctgata gcaactgacc gggcatggtc
     2281 cagatagtta tcgtcctgta aagatttgag ccttctggcg ctgataccaa tgtctgcttt
     2341 tttagctcca tcaagcaatg tctgatgcag gtaacacagt tctcccttgt catcggtcgc
     2401 cagggcataa agagactgga acatgcgccc gttataattt tgctggctgt tgaagcgaac
     2461 tccctcaacc ggaagactga atatgccacg ggaacgaaga tattcagcac cagatgttcc
     2521 ctgtagcgcc gtgagcgctg aaaatcgacg ctgaacgcgc gatctcaggc tttccgacgt
     2581 acagatcaca ggcggtttat ctctccggta ttcattcccc gtgatgcggt ctatttcagc
     2641 gcaaatttca tgaaacgctc ttttttgtgt cagagcgatc agtttcatgc cgtcaccgct
     2701 accacaaaca cagatccacg ttccggtgcc atcacggtca tcaatacgga acttacctct
     2761 ggcaccacaa accggacatt cccctttgaa gtgcttttta cccgtgactg gcggcagtcc
     2821 gtaatgtttt aaaatttcag gccagtggcc tttcgctgct tctgctgttt tcatcttact
     2881 gactcagact gtttctgttt ttctgaagtt gttgctttgc ctgcatgatc tgccacgact
     2941 cactgccttc cggcacccca gaccggacac catgaagcag ggacagagaa agcgcttcgg
     3001 gggatttttc cgtcatcttc tctgcccggc tacgccctct ggcaaaccgg atgaatttgt
     3061 gtctgatgta attactgacc tccggggtga tttccatcgg aaaactgctc agcccgtcag
     3121 gccattcgcc gaacttctcc cggaaagtgt gagcgcacca tccatcgctg accggtcgcc
     3181 ccagagaggc acgctgacgc tgatagaatt tgatctgact ccaccaggcc tgtttgtcgc
     3241 ttttggtgta aattttttcg tccttgctga gtttcctgat attgcggcgg gtatctgtgt
     3301 tcacatcctc accagccaga ggtttaaatc cgcatttcgg acagacataa acacctgccg
     3361 gtttcatgaa atgacacccc ggacattctt tcggcagttt ttcttcccga atttcgttac
     3421 cacgtgatga tgacacctgc atcccgtcgt ttttgccggg cagttcgtca tactcaatat
     3481 tttcaggaaa tcccaggcga tgaaccgtac cggaatggtc gaaaatcagg catgactcct
     3541 tgcctggcgc tgtacgcaat cctctcccaa tcgcctgaag ccagcgaatc tctgattttg
     3601 tcgggcgtgc gtaaatgatg caccggacat cactgtcaaa tcccgccacc agaacgccaa
     3661 cgctgacaat gattttcgtc gctcccgttt caaaacgact gatcattaac tgacgctcat
     3721 catgtggcgt ttcagcaacc atcacctctg cgttaattcc ggctttgttg aactgcatgg
     3781 tcacgaagct ggcatgcgtt ttattgacgc agaacgccac tgtcggtaaa tcccttccgt
     3841 tacacagcca gttatcgaca atatcgccaa ccagatccgc gccgcacata atctctgcca
     3901 gttgtgattc gtcgtaatcc ctgccgtact ccattgagac tttcgttttt acaccttcca
     3961 gatccggttt cgtgggcgca taaaattcgt aattgctcag ttcgccacgc tggatcagtt
     4021 cccctatagt ggtcggcttg agaagacgct ggtaataacg ccccaggaac gatgaaaagg
     4081 gtgtaccgga caacccaatc accttcacat ttccctctgc aaccagtcgt tcgatttccg
     4141 tgaggatgcg gcgcttacgg agatgcgctt catcaataat cagcaggtcg atattctgag
     4201 gaaaatcacg gcgaatcagc gtatccgcac tggcaatctg aatcagtcgt gacgggtcgt
     4261 tattgggatg atcacgccag ataaagctga tttcatcccc ggaaagcccg tactgaacaa
     4321 agcgctgtgc tgtctggttg atcaaaatgg tataaggaac gacaaacaga acgcgcatgc
     4381 cacggcttac cagaccagca gtgatgaacg ccgccaggcc ggttttacca ctccctgtag
     4441 gcatgtacat catgaaggac tgatatgttt tccagtcatg acgcaacata ttcagcgcac
     4501 gttcctgagc aaaattcggc ttgatattca gcatggtatt ctcccgatga cagggctgat
     4561 acagtgatta cttaaaccgg gtcgcccgga tgacatccac accatcggcg atataacgct
     4621 gtggatgttt gccatcacgg agaaaaacaa atcccgaaat gccttcagga agcgctcgaa
     4681 ttttcatcag tgaacgctga cggggcgatc ggttatccac ctcaactgcg caacgctgac
     4741 catcgggaga aataaccaga agatcgataa atcccctgcg atcacctttc agcctgacag
     4801 tgaaacagcg ttgcaactca cagcccaggg atttgatttt cttttgcaga acgtagtcga
     4861 atgtgacctt gtccattcca tgtccgaatt cctcttccag aaggttgatt agtaccagtt
     4921 tcagttcagc gctcatattt taaccatgat gtactagttt gctgatacga ccgttttttc
     4981 aggggtgccc ccctttagat cgagatctac ctaacctctg tacccgtctg ttggaagatc
     5041 ccgttccagt
//
LOCUS       DAZFRG010000304         5023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFRG010000304 DAZFRG010000000
VERSION     DAZFRG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.306"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..817
                     /locus_tag="ACHKUC_004523"
     CDS             <1..817
                     /locus_tag="ACHKUC_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6620643.1"
                     /translation="LNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHN
                     RMGQIFRKVLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAE
                     KDGYFLQRGMLLALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKL
                     NLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRA
                     NGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(863..1501)
                     /gene="ompW"
                     /locus_tag="ACHKUC_004524"
     CDS             complement(863..1501)
                     /gene="ompW"
                     /locus_tag="ACHKUC_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO6620644.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1740..1829
                     /locus_tag="ACHKUC_004525"
                     /pseudo
     CDS             1740..1829
                     /locus_tag="ACHKUC_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1857..2600
                     /gene="yciC"
                     /locus_tag="ACHKUC_004526"
     CDS             1857..2600
                     /gene="yciC"
                     /locus_tag="ACHKUC_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO6620645.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANIRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            2630..3169
                     /gene="yciB"
                     /locus_tag="ACHKUC_004527"
     CDS             2630..3169
                     /gene="yciB"
                     /locus_tag="ACHKUC_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO6620646.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3275..3673
                     /gene="yciA"
                     /locus_tag="ACHKUC_004528"
     CDS             3275..3673
                     /gene="yciA"
                     /locus_tag="ACHKUC_004528"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO6620647.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3713..4429)
                     /gene="tonB"
                     /locus_tag="ACHKUC_004529"
     CDS             complement(3713..4429)
                     /gene="tonB"
                     /locus_tag="ACHKUC_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO6620648.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTTPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            4653..4949
                     /gene="yciI"
                     /locus_tag="ACHKUC_004530"
     CDS             4653..4949
                     /gene="yciI"
                     /locus_tag="ACHKUC_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO6620649.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1246 a         1185 c         1218 g         1374 t
ORIGIN      
        1 actcaacacg ctgaaacaga aagcggcccc tctcagggta tttgatgaac gttttggcac
       61 cagaccgtta gatgccataa ccgtaaaaga tgtggtatca gtactggaag agtacaaggc
      121 cagaggacat aacagaatgg gacaaatttt caggaaagta ctgatcgatg ttttccggga
      181 agctcagcaa acgggcgatg tcccgccagg ctttaaccct gcagaatcgg caaaaaaacc
      241 gcaggtgcgg atatcaagac agcgactgac ttttgatgag tggatgatga tttataacgc
      301 agcggaaaag gatggttact ttttacagcg cggtatgctg ctggcactga tgacaggcca
      361 gcgcctttca gatatttgca aaatgcaatt ttcggatatc cgggatggtt atcttcatgt
      421 cgaacagcaa aaaacaggaa cccggattgc catccctctg gctctgcgtt gcgataaatt
      481 aaatctcacc ctggatgatg tagtgtcatc ctgccgcgat tgcgttctta gtccgtggct
      541 attgcaccac catcacgcga aagggacagc taagcgcggc gggatggtta agccagcaac
      601 attaaccgtt gcatttaaaa aagcccggga ttctgtggat tacaactggc gtgctaatgg
      661 caccccaccc tctttccatg agcagagatc tttatcagag cgattgttca gagagcaggg
      721 ggttgatacc aaaattttgc tgggccattc gaatcaaaaa atgaccgata tttacaacga
      781 cgcacgcggt aaggaatgga aaaaactggt catttgatga ccagttttgc agaggggttt
      841 tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc ggagaacata aacacccacg
      901 gatccagtcg taccgagtct tttacggtag taacaccaga tttgtactta gccgtggtat
      961 cgatatccat gtaccagacc gacatattca gcaaccagtc acggttgatg agataatcca
     1021 gacctacttg ccccgccacg ccccatgaat ccttcagact aaggtcagat aaaccaagac
     1081 cttcaccggt gtcattgaag tcattattaa aaaaggtggt gtagttaata cccgcaccaa
     1141 tgtaaggacg gaatttactg cctgaatcgc caaaatacca ttgtgccatc agtgttggcg
     1201 gtaaatggtg gacagttgca atatccccgg tagtggctgt gccaacttta tgacgaaacg
     1261 gcgatgccgc cagtaattcc acaccaatgt tgtcggtcgc catataagta aacgtcaggc
     1321 ccagttgagt gttattggtc acgctgaatc cacccagact tcctaacgta ccaccagcac
     1381 cttctgttgg acgtacggtt gcagaacctg cacgcataaa aaattcgccg gcttcatgcg
     1441 caaaggcact gccagagaga agagttgcta ctgccaaagc cgccactgtt aactttttca
     1501 tatccgctcc gtcgttatgg ttataaaaac gtgacgaata tacccacatt ggggtaacaa
     1561 atgatcctac acagatcaca ttacatatgg taattcaccc tttattgatc tggattaatt
     1621 tagaagttgc attgaaaatc ctggttaatg tgatttaaat caatttttat atatttcgct
     1681 acgcaaatat taactcgctc gccacttgca gcggtttgcc cacgcctgaa ggccagcaat
     1741 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccggggct
     1801 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1861 ctatcacggc gcagtccgta taccgtgaca ccggaaattt tttccgtaat caatttatga
     1921 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     1981 cacccagtga tgcacagctt gcgcagctca atgacggcgt acccgttagc ggcagtagtg
     2041 ggttgttcga cctggttcag aatatgtcac cggaacagca acaaattttg ctgcaggctt
     2101 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccggg ggcgtaatat
     2161 taattatcca gctggtgtct gcgggtcaga gagtcagtgc actgcgagct attggtgcca
     2221 gtgcgccgat attgccaaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2281 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gctctggcac
     2341 cggtgatgct ggtacaggac aaaatgggcg tttttgcctc gatgcgtagc agtatgcggc
     2401 tgacatgggc gaatatacgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2461 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2521 tggcgaacac cttgagcaac ctgatttcag ccgtattgct catctatctg ttccgcctgt
     2581 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2641 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2701 ggctactgcg gcgctcatcg tcgccacggc gattgtgctt atatatagct gggttcgctt
     2761 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2821 aacgctgttc ttccacaatg atgagtttat taaatggaag gttactgtca tttatgccct
     2881 gtttgcgggt gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     2941 gctgggtaaa gaactcaccc tgccgcaatc ggtatggtcg aagctgaatc tggcctgggc
     3001 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3061 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3121 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3181 gtccttcagg ggctggcgct gtatctcctc tgttctcctc aggaaaatca tagtagcatc
     3241 gcgcctgtga ttttcctttt aagtcggttt taccatgtct acaacacata acgtccctca
     3301 gggcgatctt gttttacgta ctttagccat gcccgccgat accaatgcca atggtgacat
     3361 ctttggtggt tggttaatgt cacaaatgga tattggcggc gctattctgg cgaaagaaat
     3421 tgcccacggt cgtgtagtga ccgtgcgggt tgaaggaatg actttcttac ggccggttgc
     3481 ggtcggggat gtggtgtgct gctatgcacg ctgtgtccag aaagggacga catcagtcag
     3541 cattaatatt gaagtgtggg tgaaaaaagt ggcgtctgaa ccaatcgggc aacgctataa
     3601 agcaacagaa gcattattta agtatgtcgc ggttgatccc gaaggaaaac ctcgcgcctt
     3661 acccgttgag taataatcaa aagcctccga tcggaggctt ttgactttat gcttactgaa
     3721 tttcggtagt gccgttaatt ttaaacagga tattcaccac aatcccactg cctggcttac
     3781 ccggctcata acgccatctg cgcatcgcat ttttcacctc acgctcaaac atgtttgcag
     3841 gcttggctga gaggatttgt acgttatcca cgcgaccatc tggcgtgaca tcaaatttaa
     3901 ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc cggatactgc ggctgattac
     3961 ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg cttgctggtt gcagccgttg
     4021 ccgtacttga tgtcgggcgc gcgggtgtcg tattttcaaa cggtgatgcc ggacgcgact
     4081 ctacgggttt gacatcgcgt tttggctgct ccaccttttt caccggcttc ggttttggct
     4141 taggtttcgg cttcggcttt tcaatgacca ccggagcttc ttttggcggt tcagggatcg
     4201 gctcaggttc cggttctggc tctaccaccg gctccggtgg tggctgaacg gcttgtggcg
     4261 gttcaagatc agcaggcgca accatcgtga cagaaatcgg ctgcgcaggc gcaggtagtt
     4321 caataacctg atgtaccgag gtatagagca gacccgccac aacagcacca tgaatgcaga
     4381 ccgaaagtaa cgtcggccat gggaagcggc gaggtaaatc aagggtcatt gaagtcataa
     4441 tcatttcagt agaaaaacca ggccccgatt ttaaatgcaa atagcaatca tattcaataa
     4501 ggcaagacga tcaggatcag tgaaaatgtc atttttggcg taattgacct tactttatgc
     4561 atggtcttaa caaagacatc atctttccgt tgcagatctt cgccctttca aataacgtac
     4621 tttacaactt tcccgaacaa ggagttgtgc ccgtgttgta tgttatttac gctcaagaca
     4681 aagctgatag cctcgaaaaa cgcctttccg ttcgtccggc acatttagca cgtttacagt
     4741 tactgcatga tgaaggtcgt ttgttgaccg ccggtccaat gccagcggta gacagtaacg
     4801 atccaggggc tgcgggattt actggctcaa ccgttattgc tgaatttgag tcgctggaag
     4861 cagctcaggc ctgggccgat gctgacccgt acgtagcggc aggagtctat gaacacgttt
     4921 cagtgaaacc atttaagaaa gtgttctgaa tagagattca atatttaagg gcacggtttt
     4981 tgcaaccgtg ttcttgtttg gcctttcctt ataggtcggt cag
//
LOCUS       DAZFRG010000305         4979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFRG010000305 DAZFRG010000000
VERSION     DAZFRG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.307"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..609
                     /locus_tag="ACHKUC_004531"
     CDS             88..609
                     /locus_tag="ACHKUC_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6620650.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGVQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQTQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            666..899
                     /locus_tag="ACHKUC_004532"
     CDS             666..899
                     /locus_tag="ACHKUC_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO6620651.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGAHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            960..2919
                     /locus_tag="ACHKUC_004533"
                     /pseudo
     CDS             960..2919
                     /locus_tag="ACHKUC_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            2974..3408
                     /gene="psiB"
                     /locus_tag="ACHKUC_004534"
     CDS             2974..3408
                     /gene="psiB"
                     /locus_tag="ACHKUC_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6620652.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3405..4167
                     /locus_tag="ACHKUC_004535"
                     /pseudo
     CDS             3405..4167
                     /locus_tag="ACHKUC_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            4341..4493
                     /locus_tag="ACHKUC_004536"
                     /pseudo
     CDS             4341..4493
                     /locus_tag="ACHKUC_004536"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4438..4560
                     /locus_tag="ACHKUC_004537"
     CDS             4438..4560
                     /locus_tag="ACHKUC_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6620653.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
BASE COUNT         1112 a         1321 c         1560 g          986 t
ORIGIN      
        1 ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct
       61 gaaaacatca acagaaggag atacatcatg gcagttcgtg gcattaacaa ggtcattctt
      121 gtcggtcgtc tgggaaaaga tccggaagtc cgttacatcc ccaacggggg cgcagtggca
      181 aacctgcagg tggccacgtc agaaagctgg cgtgacaaac agacggggga gatgcgggag
      241 cagacagaat ggcatcgcgt ggttctgttc ggcaagctcg cggaagtggc tggcgaatat
      301 ctgcgcaagg gcgtgcaggt ctacatcgaa ggtcagcttc gcacccgtag ctgggaagat
      361 aacggtatca cccgttacgt caccgaaatt cttgttaaga ccacaggcac catgcagatg
      421 ctggggagtg caccacagca gaacgctcag acgcaaccgg agcctcagca gaatgggcag
      481 ccacagagtg ctgacgcgac gaaaaaaggt ggcgcgaaaa cgaaaggccg tggacgtaag
      541 gccgcgcagc ccgagcctca gcagccgtct gaacccgcgt atgacttcga tgacgacatt
      601 ccgttctgaa cgactgacag tgacaaccgc cccgtccagt gcggggcatc accggagaga
      661 tgaggatgag cgaatatttc agaatatttc aggggctgcc tgacggctcc tttacccgcg
      721 aacaggctga agccgttgcc gcacagtacc ggaatgtctt tatcgaggat gatcagggag
      781 cgcattttcg cctggttgtc cgcaataaca gtggcctggt ctggcgtacc tggaattttg
      841 aggacggtgc cgggtactgg atgaaccatg tcatccggaa tttcgggatc cttaagtaag
      901 agaggtgccg gacggggatc acgtccggca gacattaact acacaataag gggatagtca
      961 tgtcagtagc aaagtctgaa ccaaaatcta cccgtaaagc aagccgtaaa cctgcaaaaa
     1021 cgcaggaaac tgtcctgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg
     1081 cctcactgat taaatcaccg ctgaatgtgc gcacggtgcc gtattctgcg gagtccgtca
     1141 gcgaactggc ggactccatt aaaggtgtcg gcctgttaca gaatctggtt gttcatgccc
     1201 tgccaggtga ccgttacggt gtcgccgcag gcggtcgccg actggcagca ctcaacatgc
     1261 tggcagagcg caacatcctt ccggctgact ggcctgtacg cgtgaaggtc attccgcagg
     1321 agctggcgac tgccgcatcg atgaccgaga acggtcagcg tcgggatatg caccctgccg
     1381 aacagattgc cggattccgc gcaatggcgc aggaaggcaa aacacctgca caaatcggtg
     1441 atttgctggg ctattcaccc cgccacgttc agcgaatgct gaaactggct gaccttgcgc
     1501 ctgtcatcct cgatgcgctg gcagaagacc gcatcaccac cgagcactgt caggcgctgg
     1561 cgctggagag cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgc cagtcaggat
     1621 ggggcggtaa accggaagtg cagaccattc gtcgtctggt gaccgaaagt gaagtggcgg
     1681 tggcggggaa cagtaaattc cgcttcgtgg ggggctgatg ccttctcgcc agacgaactg
     1741 cgcaccgatt tgttcagcga tgacggggac ggttatgtcg accgcgtggc gctcgatgcc
     1801 gccctgctgg aaaaactcca ggctgtcgca gagttccttc gggaagccga aggctgggga
     1861 tggtgcgccg gacgcatgga gcctgtcggt gagtgccgtg aggatgccgg aatataccgc
     1921 tgtctgccgg agccggaagc ggtgctgacg gaggcggaag aagaacgtct gaacgaactg
     1981 atggctcgtt acgacgcgct ggaaaaccag tgtgaggaat ctgacctgct ggaagcagaa
     2041 atgaagctga tgcgctgcat ggcgaaggtc agagcgtgga cgccggagat gcgtgccgga
     2101 ggtggagtgg tggtgtcctg gcgttatgga aacgtatgtg tccagcgtgg tgtgcagtta
     2161 cgcagtgagg atgatgcggc tgacgacgct gaccgcacgg aacaggtgca ggaaaaagcg
     2221 tcagtggagg aaatcagtct gccactgctg acgaaaatgt cttccgaacg cacgctggca
     2281 gtccaggcag cactgatgca gcaaccgtac aaatccctga cactgctggc atggacgctc
     2341 tgcctgaatg tgtttggcag cggagcgtac agtaaaccag cacaaatcag cctggaatgt
     2401 aaacattatt cgctgaccag cgatgcgcca tcggggaagg aaggtgccgc attcatggcg
     2461 ctgatggcag aaaaagtccg tcttgctgcc ctgttaccgg agggatggtc acgggacatg
     2521 acgacattcc tgtcactcag tcaggaggtg ctgttatccc tgctcagttt ctgcaccgca
     2581 tgcagcctta acggtgtcca gacccgtgag tgtggtcaca cgtcacgcag tccgcttgac
     2641 acgctggaaa ccgctatcgg attccacatg cgcgactggt ggcagccgac aaaagcaaac
     2701 ttcttcggat acctgaaaaa gccgcagatt atcgcagctc tgaatgatgc cggactgtcc
     2761 ggtgccgcac gggatgcaga gaggatgaag aaaggtgatg cggctgaaca tgcagagcac
     2821 catatgaaag acaaccgctg ggtgcctggc tggatgtgtg caccacgtcc acagacggat
     2881 accactgaac gcaccgataa cctggctgat gccgcctgat gaacaaccac accgccccgc
     2941 cggagacggg gcggcagcaa gggagatacc gtgatgaaaa ctgaactgac cctgaatgta
     3001 ttacagacca tgagtgcaca ggaatatgaa gatatccggg ctgccggaag tgatgaacgc
     3061 cgtgagctga cacacgccgt gatgcgggaa ctggatgcgc cggacaactg gacgatgaat
     3121 ggcgagtacg gcagcgagtt cggcggcttt ttccccgtcc aggtccgttt cacgcctgcc
     3181 cacgaacgtt ttcacctggc gttatgttca ccgggcgatg tctctcaggt ctgggtgctg
     3241 gttctggtga atgccggtgg tgagccgttt gcggtggttc aggtgcagcg gcggtttgta
     3301 ccggaagccg tcagccattc actggcactg gcggcgtcac tggatgcgca ggggtacagc
     3361 gtcagtgaca tcatccatat cctgatggcg gaaggaggtc aggcatgagc gtacgttcac
     3421 aggcactggt tcccctcagc gcagagcaac aggccgcatg gcgggcggtg gcggagacgg
     3481 aaaaacgccg ccatcagggt aacacgctgg cggaatatcc gtatgcgggg gcgtttttcc
     3541 gctgtctgaa cggaagccgc aggatatcgc tgtctgacct gcgatttttc atgccgtccc
     3601 tgaccgcaga agaactgcac ggcaaccgtc ttcagtggct gtatgccatc gatgtgctga
     3661 ttgaaacaca gggcgaggtc tgcctccttc cccttccagg cgatgcagca gaacgcttgt
     3721 tcccgtcggt gcggtttcgt gtcagggaac gcagtcgcca taaatccgcg ctggtcatgc
     3781 agaagtacag ccgccagcag gcgagggagg cagagcagaa agcccgtgcg taccaggcgc
     3841 tggtcgctca ggctgaaatc gaactggcat tccattcgcc ggaaacagtc ggaagctggc
     3901 atgcacgctg gagtgaccgg gtggcagagc atgacctgga aaccctgttc tggcagtggg
     3961 gcgagcgttt tccgtcactg accgggatgg aacgctggca gtggcaggat atgccattct
     4021 ggcaggtcat cgcagaggct ggtatggcgg caagggaggc cggtcatgcc gtccgtgaga
     4081 tggagcgatg gatggtgccg aacaaactcc gggaggcggc gtgatgcggc aacaatcaca
     4141 cagattaccc gtaaacagcc tgaatgagcg ggttattttc agggaaagtg agtgtggtca
     4201 gtgcgcaggt atatgggcta tgatgtgccc ggcgcttgag gctttctgcc tcatgacgtg
     4261 aaggtggttt gttaccgtgt cgtgtggcag aaggacaaaa gccccgtagt taatttttca
     4321 ttaacccacg aggcctctgc atgtctagtc cacgtcagta tggcctctta ccgtgcaaat
     4381 tgcaaggagg agtaaaccat gaaactaccg ggaaatgccc ttatctggtg cgtattaatc
     4441 gtgtgctgca cgttgttgat attcacactc ctgacccgga atcgtctgtg cgaagtccgg
     4501 ctgaaggacg gatacaggga agttacggca acaatggctt acgaatccgg cggtaagtag
     4561 caacctggag gcgggcgcag gcccgccttt tcagggatga tgctggtctg actgcactga
     4621 agcgccttta taaaggggct gctggttcgc cggtggcccc tttctccttg ctggcattgt
     4681 gcgggcatga acaaccagac tgccaggaag gccgtttccc tccgggaacg gggaagaacg
     4741 cgcgcctgcg gcgcccgttc ttttcccccg ccctctctgt ttatggccag ttgtcagaaa
     4801 tgacattacc tttcccgtga ttattctctt tttcttcctg ctctgattct gactactgca
     4861 gtgttgttct gtctgccggg cgcaccttcg gctcccggct gcctggcggc atccggtcgt
     4921 caggcccgac tccaacaggc agctgctggc gtacctgcca gtctccgccg aacccgctg
//
LOCUS       DAZFRG010000306         4968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFRG010000306 DAZFRG010000000
VERSION     DAZFRG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.308"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..200
                     /locus_tag="ACHKUC_004538"
     CDS             <1..200
                     /locus_tag="ACHKUC_004538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620654.1"
                     /translation="SIRTALQAYLFLIFIKMLRQLMFLLPIKFQRGKKLRTRRYRGLQ
                     SKEKRKLSLLTTTFCDEVVGG"
     gene            612..2594
                     /gene="chuA"
                     /locus_tag="ACHKUC_004539"
                     /gene_synonym="shuA"
     CDS             612..2594
                     /gene="chuA"
                     /locus_tag="ACHKUC_004539"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO6620655.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            2643..3648
                     /gene="chuS"
                     /locus_tag="ACHKUC_004540"
                     /pseudo
     CDS             2643..3648
                     /gene="chuS"
                     /locus_tag="ACHKUC_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
     gene            complement(3705..4235)
                     /gene="dctR"
                     /locus_tag="ACHKUC_004541"
     CDS             complement(3705..4235)
                     /gene="dctR"
                     /locus_tag="ACHKUC_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6620656.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(4388..4954)
                     /gene="slp"
                     /locus_tag="ACHKUC_004542"
     CDS             complement(4388..4954)
                     /gene="slp"
                     /locus_tag="ACHKUC_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO6620657.1"
                     /translation="MNMTKGALILSLSFLLTACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1326 a         1104 c         1213 g         1325 t
ORIGIN      
        1 attcaatacg cacggcgtta caagcgtatt tatttttgat ttttatcaag atgcttcgtc
       61 agctaatgtt cttgttaccg attaaatttc agcgcgggaa aaagctgaga acccggagat
      121 atcgcggctt gcagagcaaa gagaagagaa aattatcact gttaacgaca acgttttgtg
      181 atgaggttgt ggggggatag ccataaacac aggatggttt tctggatgct ggatagattt
      241 cataaccccc tgtttattgt gttaatggcg gttaaaaaaa gcttgatcat aaaaaattga
      301 tcgataataa ttctcattat cacgtttgag tttaacaaaa tatgacatca tggataacaa
      361 aatcatatct tttgtaaggg aaaacgcaca gcactcttca cttcagacgt ggcgtactac
      421 tttgcctgac aaagagggcg tgcttttgct gaattatcag aaatattcgg caattttacg
      481 ggatatatac gctaatagct tcccgtggtg atatctaatc atttgaattg taaataaatt
      541 tatttttcgc cagccacccc ggtattaacc caggcagcca gcatttttta ttttcctcat
      601 ggagaatcgc tatgtcacgt ccgcaattta cctcgttgcg tttgagtttg ttggctttgg
      661 ctgtttctgc caccttgcca acgtttgctt ttgctactga aaccatgacc gttacggcaa
      721 cggggaatgc acgtagttcc ttcgaagcgc ctatgatggt cagcgttatc gacacttccg
      781 ctcctgaaaa tcaaactgct acttcagcca ctgatttgct gcgtcatgtt cctggaatta
      841 ctcttgatgg taccggacga accaacggtc aggatgtaaa tatgcgtggc tatgatcatc
      901 gcggcgtgct ggttcttgtc gatggtgttc gccagggaac ggataccgga cacctgaatg
      961 gcacttttct cgatccggcg ctgatcaagc gtgttgagat tgttcgcgga ccttcagcat
     1021 tactgtatgg cagtggcgcg ctgggtggag tgatctccta cgatacggtc gatgcaaaag
     1081 atttattgca ggaaggacaa agcagtggtt ttcgtgtctt tggtactggc ggcacggggg
     1141 accatagcct gggattaggc gcgagcgcgt ttgggcgaac tgaaaatctg gatggtattg
     1201 tggcctggtc cagtcgcgat cggggtgatt tacgccagag caatggtgaa accgcgccga
     1261 atgacgaatc cattaataac atgctggcga aagggacctg gcaaattgat tcagcccagt
     1321 ctctgagcgg tttagtgcgt tactacaaca acgacgcgcg tgaaccaaaa aatccgcaga
     1381 ccgttgaagc ttctgatagc agcaacccga tggtcgatcg ttcaacaatt caacgcgatg
     1441 cgcagctttc ttataaactc gccccgcagg gtaacgactg gttaaatgca gatgcaaaaa
     1501 tttactggtc ggaagtccgt attaatgcgc aaaacacggg gagttccggc gagtatcgtg
     1561 aacagataac aaaaggagca aggctggaga accgttccac tctatttgcc gacagtttcg
     1621 cttctcactt actgacatat ggcggtgagt attatcgtca ggaacaacat ccgggtggcg
     1681 cgacgacggg cttcccgcaa gcaaaaatcg attttagctc tggctggcta caagatgaga
     1741 tcaccttacg cgatctgccg attaccctgc ttggcggaac ccgctatgac agttatcgcg
     1801 gtagcagcga cggctacaaa gatgttgatg ccgacaaatg gtcatctcgt gcggggatga
     1861 ctatcaaccc gaccaactgg ctgatgttat ttggctcata tgctcaggca ttccgcgccc
     1921 cgacgatggg cgaaatgtat aacgattcta aacacttctc gattggtcgc ttctatacca
     1981 actattgggt gccaaacccg aacttacgtc cggaaactaa cgaaactcag gagtacggtt
     2041 ttgggctgcg ttttgatgac ctgatgttgt ccaatgatgc tctggaattt aaagccagct
     2101 actttgatac caaagcgaaa gattatatct ccacgaccgt cgatttcgcg gcggcgacaa
     2161 ctatgtcgta taacgtcccg aacgccaaaa tctggggctg ggatgtgatg acgaaatata
     2221 ccactgatct gtttagcctt gatgtggcct ataaccgtac ccgcggcaaa gacaccgata
     2281 ccggggaata tatctccagc attaacccgg ataccgttac cagtaccctg aatattccga
     2341 tcgctcacag tggcttctct gttggttggg tcggtacgtt tgccgatcgc tcaacacata
     2401 tcagcagcag ctacagcaaa caacctggct atggtgtgaa tgatttctac gtcagttatc
     2461 aaggacaaca ggcgctcaaa ggcatgacca ctactctggt attgggcaac gccttcgata
     2521 aagagtactg gtcgccgcaa ggcatcccac aggatggtcg taacggaaaa attttcgtga
     2581 gttatcaatg gtaatcatct gccccgatat ttcggggcat ttatctggaa ggaagagaga
     2641 caatgaacca ctacacacgc tggcttgagt taaaagaaca aaatcctgga aagtacgcgc
     2701 gtgacatcgc aggtttaatg aatatcagcg aagcagaact ggcatttgcg cgcgtcacgc
     2761 acgacgcgtg gcggatgcgc ggcgatattc gtgaaattct ggcggcgctc gaaagtgttg
     2821 gctgaatatg cagtccatga gcaagttggt gcgttcacaa accagcattt gaacggacat
     2881 gccggattga tcctcaatcc gcgcgcgctg gatttacgtc tgtttctcaa tcaatgggcc
     2941 agtgttttcc acatcaaaga aaacacggct cgtggcgaac gccagagtat tcagttcttt
     3001 gatcatcagg gcgatgcatt actaaaagtt tatgccaccg acaataccga tatggcggca
     3061 tggagtgagc ttctggcacg gtttatcacc gatgagaata cgccgcttga gttaaaagcc
     3121 gttgatgcgc cagttgttca aacgcgagcc gatgccagtg tggtcgagca agagtggcgg
     3181 gcgatgaccg acgttcatca gttttttacg ttgctcaagc gccacaacct gacgcgccaa
     3241 caggcgttca atctggtggc agacgatttg gcctgcaaag tatccaacag tgcgttggcg
     3301 caaattcttg aatctgcaca gcaggatggc aatgaaatca tggtgtttgt tggcaaccgt
     3361 ggctgcgtac agattttcac cggtgtggta gaaaaagtgg tgccaatgaa aggttggctg
     3421 aatattttca acccgacgtt tacccttcat ctattagaag agagcattgc tgaagcctgg
     3481 gttacccgta aaccgaccag cgatggccac gtaaccagcc tggaattgtt tgcccatgat
     3541 ggtacgcaga tagcgcaact ttatggtcaa cgtacagaag gtgaacagga gcaagcgcaa
     3601 tggcgtaagc aaattgcttc gttgataccg gaaggcgtta ctgcataacg tataattaac
     3661 gggaggtctg gctgttgctg ggcctcttta tattgttcaa tcgttcacac cagataatta
     3721 atatgctgat ggcgtaccag ctcattgatc cgcttaagat tgaggatcat catgatattg
     3781 tgtttatggc agtagaacgt ttttagcgat attttcagtt tctccagaat atcttccgtt
     3841 gtcatttgat tcgcaataca gctcagaacc tgatgctgct tgcgcgagag ggacacatcc
     3901 gcttcaggca cagagcagta actgccattg atgagtgcga caaagtcgat gatggcgata
     3961 gttcgcggaa caaaggtcac cggtactttc cccaggcagc gtttaatatt aaagggggag
     4021 aaaataatta catggacagg ttttaatcgt tcaatttgag tgagaaaatt agaatgaaat
     4081 acattattca ttaatgtatc aataatgacg aaggcattct gatgcaacat tacgtcgatc
     4141 tcttcttcgt tctgtatatg aacgacatta cctttactca gaatgttttt catcgcagtg
     4201 aaaaacatcg tatccctggt aattataaga aacataaccg actcctggta cggacattaa
     4261 ctttataatc agtttgccaa aggtaattaa caggacaggg cttataataa tagttagctt
     4321 actgtgtatt aatttgatgt aatgcatgat gcagatgctg agaaagcatc tgcatctttc
     4381 ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg tagtaaggag
     4441 ccccccagcc cggttccggc cagaatgcac catagccgta atcccacagg ttataggtgg
     4501 tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact tccaggaagt
     4561 tatacgggac tttattgata aagccaggtt gttcaccctg aatggtaccg aggatggtaa
     4621 caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg agcagtcggc
     4681 cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat acagcgattt
     4741 ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccgcca aagcgcgctt
     4801 gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt tgaatatcag
     4861 gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggtaagc aaaaatgaaa
     4921 ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttat
//
LOCUS       DAZFRG010000307         4946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFRG010000307 DAZFRG010000000
VERSION     DAZFRG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.309"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..1747)
                     /gene="glnS"
                     /locus_tag="ACHKUC_004543"
     CDS             complement(83..1747)
                     /gene="glnS"
                     /locus_tag="ACHKUC_004543"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO6620658.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1967..2728)
                     /locus_tag="ACHKUC_004544"
     CDS             complement(1967..2728)
                     /locus_tag="ACHKUC_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6620659.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2730..3716)
                     /locus_tag="ACHKUC_004545"
     CDS             complement(2730..3716)
                     /locus_tag="ACHKUC_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HFO6620660.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTASKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3709..4944)
                     /locus_tag="ACHKUC_004546"
     CDS             complement(3709..4944)
                     /locus_tag="ACHKUC_004546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFO6620661.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTVIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
BASE COUNT         1150 a         1364 c         1244 g         1188 t
ORIGIN      
        1 aacgccgcag agcgcggcgt ttgggataat ttgcatattt cttgccggat gcggcgtaaa
       61 cgctctatcc gacaacgtaa acttactcgc ctactttcgc ccaggtatca cgcagcccaa
      121 cggtgcggtt aaataccggt ttttccgccg tagaatggcg gctgtcgagg cagaagtaac
      181 cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa
      241 agccctgttt gatcaccagc gactccgggt taatcaccga caggaaatca tccgcagcac
      301 cagggttcgg cacgctgaac agacgatcgt acaggcggat ttcaaccggc agcgcatgtg
      361 ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta
      421 aggtgtcggc gtcataagta cagaagatgg tggtgatatt gccttcggca tctttctcca
      481 cgcgttctgc cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac
      541 gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atctcaccgc
      601 taaatggcac ctgacggctg cccatttccg gtttattcgg atggttcggc atggtaacca
      661 tttcgccttc gccctggtag ttttcgataa ccagtttcac cggatcgata accgccattg
      721 cgcgcggcgc attttcgttg agatcttcac ggatgcagga ttccagtgac gccatctcaa
      781 tggtgttgtc ctgcttggtc acaccaatgc gtttgcagaa ctcacgaata gaagccgcag
      841 tgtaaccacg acgacgcaga ccagaaatgg tcggcatacg cgggtcatcc cagccttcaa
      901 cgtgcttatc ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat
      961 tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg tccagtaccc
     1021 agtcgtacag acggcggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt
     1081 ccagcgcatc gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc
     1141 cagtctggtg gtgttcagca aacttaatgc ggtacagcac cggatcgcgc atcacgataa
     1201 acggcgaagc catgtcgatc ttcgcacgca ggcaggcttt accttcttca aaaccgccgg
     1261 tacgcatttt ctcgaacagc gccaggttct cttcaacgct gcgatcgcgg tacgggctgt
     1321 ttttacccgg ctgcgtcaag gtgccgcggt attcgcggat ctgttccggc gtcagttcat
     1381 caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat
     1441 aatcggagga gtaacggacg ttaccagacc agtggaaacc taaccactct acgtcgtttt
     1501 tgatcgaatc gacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt
     1561 tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc
     1621 caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac
     1681 cactggccag atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct
     1741 cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg
     1801 ggacaggctg acaactgtta gttagcgaaa aaatggtttt gcgtaagata ttggggcgtt
     1861 ttctgcgcta ctgccagcag atcctcgcgc tggaaacgta aggccaaacg tcgccagcct
     1921 gacgcattta ttaaattgcc ggatgagctg gccgcagagt catagattag tcggctatga
     1981 ttaaacgcgt cgacaaggac gcaagcgttc tacgcgtctg cccatcaagc tgactatccg
     2041 taataatctc tgtcagttca ttaagatgcg cgatattaaa cagcgaccat gaaccgtatt
     2101 ttgagctgtc agccaggaga attttgcgcg aagcatgcgc gataagatcg cgttttagtg
     2161 cggctttctc ttccgtgggt gctgtaatac ccttttcaag atcccagctg ttacagctta
     2221 aaaatgccac gtccggccag gtattttgca gcaatttacg accatgttcg ccaatgcagg
     2281 actggctgga gtcgtcgata cgtccgccga taatcgtcac ttcaatttgc ttgaattcag
     2341 ccaaaaaaag cgcgatatgc aggtcgcttg tgatcacgcg aagcggtaaa tgtgtaatct
     2401 gccgcgcaag ttcaatcatt gtcgtaccgg catcaaggac gacggaatcc cccgctttga
     2461 ccattgacgc cgctgcgctg gcgatggcat gtttttccgc caggctgcgc tgcattttct
     2521 catgggtagt cggttgggta ggaataaacc ggttgagggt cacaccgccg tgtgtacggc
     2581 tgataacacc ctgctcatcg agttttatca ggtctcgacg aatcgtagcc ggcgacgcat
     2641 tggttaccgc caccagctca tcaacggtga ccagattatg gcttttcagg taatccatga
     2701 tctgctctaa ccggttatat cccttcattt tattctcgcg aaatctgcat tgctaactgg
     2761 atggaaactg ccatgctctc agacttggct tttcctgtcc aggcaatatc aaacgcggtg
     2821 ccgtggtcgg cagaagtgcg aataaatggc aacccggcgg tgatattcac cccatcataa
     2881 aatccaagca gttttaaagg gatatgcccc tgatcgtggt acatagccac taccatatcg
     2941 aatagccctt catggcattg cataaacacg gtatctggcg ggcacgggcc agtaacatca
     3001 atatcttcgg cctgcattgc ttcaatcgca ggcgcgataa tctcgatttc ctcagtgccg
     3061 aataaaccat gttcccctgc atgtggattt acacccgcca cggcaatacg cggacgctca
     3121 ataccaacac gtttcaggaa gtggttcgca acacgaataa ccgttttgac cctttcacca
     3181 ttcaaggtat ccaggaattt acgcagtgag atatgcgtcg aaatatggat aaccttgagc
     3241 ttatcggtat agagcaccat cgcgtactct ttgctggcgg taagatgggc cagcaattcc
     3301 gtatgccccg gatagttatg cccgccaaga tgcagcgctt ctttattcag cggcgcagtg
     3361 gctatggctt tcacctcgcc tgataaggcc agagccgttg cacgtttgat acagcgatac
     3421 gctaaatcgc ctgccacagc ctgtaccact ccgggtgtca gcgcatcggg gttagccagc
     3481 ggctcatcca tcacgttaat cacgccggga gcaaaatggg cgtcgcacac cttttgaatg
     3541 acgcgtaact ctgcctgcgg cgtgatattc atcgccataa tacgtcgcag cgtacgcaca
     3601 cagcccacca ccaccaccgg ggcaccagca agcccggctt cgctcaggga tttaataatg
     3661 atctccgggc caatacccgc cgggtcaccc atcgttaccg caataatatt attcactaca
     3721 cttctcctcg ataaaattca gaacctgatg caacgtggtc tcatcgccaa acccaccggc
     3781 tttcgtcatc accgaacggg accagacacc gcccagaaag tgaccgtatg gaacgcattg
     3841 cgccaccttc cccgtaatac gaaatccggt cgcgcctaac gcactggctg ttgccattgc
     3901 gacatcacca ccagagagat aaagcgcatc gggcattgtg cgaagcagaa cttgccgggt
     3961 aagttcgcca agaaaccggc aaattttttc cccaagtgca gcgcgactca tctgccaacg
     4021 ctggcaaagg gtgtcgatct gatgtcttgc tacagaatcg ttacaggtgt ggacaatgca
     4081 gtggtcgcct ttctgcaagg cctgcacaat acgcgcgtca tagtcactgg cattgcctgc
     4141 cagaatgtgt tcaacatcga cgtatatctg tgtgatacgt gggtgtgaat gaagtgtggc
     4201 gatctgccgt tgagcgattt cgctcattga accaataatg gccagcacgg tgggtggcgg
     4261 cataatcgcg gagcgccggg caacggcatc acaaatacca gcggagccga ccagcagcag
     4321 cggttgttta gcctgcatca cagcggcagc gatgcgatcc aggtcgctgt ccgtctgcgc
     4381 atctatcacg ccaatccacg gttgctcata agacaaatgg ctgagctgag aaaccgtaac
     4441 gggctggcat ggaatgaccg tctgcaaccg gatgatgtcc gcaattcggg cactcaatac
     4501 cggggttttc ggatcgctgg caaactcggt ttccgttatc cgcacaccgt ttacccaaca
     4561 ctcaccggcc actgtggtcc ttcccgcctg aggaaatgcg ggggcaacca ctgcgcccgt
     4621 tataccgcat gccttcatca gggcttcaat ttctgcgccg atattgcctc gcagcgtgga
     4681 atctatcttt ttaatcacga gcggcgggtt attcgccaga gggagatgac tcatcagcga
     4741 ggttattttc atcgccgcca gcttaggatc catcgcccgg ctgtcgctgt taatcaccca
     4801 tacgctggca tcaccgcgat aaggcatttc aaaggccaca tcaactgtat gccccacctg
     4861 cgccaggctt acccccgcgt cgttggctcc cgtgaaatca tcggcaacga cgagaatgct
     4921 cggcttagtg attccctttt ccatca
//
LOCUS       DAZFRG010000308         4823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFRG010000308 DAZFRG010000000
VERSION     DAZFRG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.310"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(148..224)
                     /locus_tag="ACHKUC_004547"
     tRNA            complement(148..224)
                     /locus_tag="ACHKUC_004547"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Met,seq:cat)
     gene            433..1530
                     /gene="mltA"
                     /locus_tag="ACHKUC_004548"
     CDS             433..1530
                     /gene="mltA"
                     /locus_tag="ACHKUC_004548"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO6620662.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1607..2413
                     /gene="tcdA"
                     /locus_tag="ACHKUC_004549"
     CDS             1607..2413
                     /gene="tcdA"
                     /locus_tag="ACHKUC_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO6620663.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     MVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2464..2907)
                     /gene="csdE"
                     /locus_tag="ACHKUC_004550"
     CDS             complement(2464..2907)
                     /gene="csdE"
                     /locus_tag="ACHKUC_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO6620664.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TTAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2907..4112)
                     /gene="csdA"
                     /locus_tag="ACHKUC_004551"
     CDS             complement(2907..4112)
                     /gene="csdA"
                     /locus_tag="ACHKUC_004551"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO6620665.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4304..4531
                     /gene="ygdI"
                     /locus_tag="ACHKUC_004552"
     CDS             4304..4531
                     /gene="ygdI"
                     /locus_tag="ACHKUC_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO6620666.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         1163 a         1205 c         1297 g         1158 t
ORIGIN      
        1 atatataaat aagatggatg gctttgatgg agggatatta acggtttact cattgtctca
       61 ggcttcactg aaacgggtaa ggaagcgtaa aaacgaaaaa accgcaagcc aatagtagct
      121 ttgcggtttg tcagcattct aataaattgg ttgcgggggc cggatttgaa ccgacgacct
      181 tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgtccgtg gatgcgcact
      241 atactctgct cgcgttttca tgcaaccttt tttttacccc gatcacggat ttggcatgat
      301 tttgaacaaa aacaaatcac taaagcctgt tttttgtgca aaatttgcgt cagggagagg
      361 ctgctcattt cattaaacgc ggtggtttgt tatcttcgtt gcgccttatt ttttaacctg
      421 aagaagagaa caatgaaagg acgttgggta aagtaccttc ttatgggcac ggttgtggca
      481 atgcttgccg cctgctcttc caaaccaacc gatcgcggac agcaatataa agacgggaaa
      541 tttacccagc ctttctctct ggtgaaccag ccagatgccg ttggcgcgcc gattaatgcc
      601 ggtgattttg ccgagcaaat taaccatatc cgtaattcgt caccgcgtct gtatggcaac
      661 cagagtaatg tttataacgc ggtgcaagag tggctgcgcg caggcggtga tacccgcaat
      721 atgcgccagt tcggcattga tgcctggcag atggaaggtg tcgacaacta tggtaacgtg
      781 cagtttacag gctattacac gccggtaatt caggcgcgcc atacccgtca gggcgagttc
      841 cagtatccga tttatcgtat gccgccaaaa cgtggtcgtc tgccgtctcg tgcggagatc
      901 tacgcaggag cgctgagtga taaatatatt ctcgcttaca gtaactccct gatggataac
      961 ttcattatgg atgtgcaggg tagcgggtat atcgactttg gtgatggcag tccgcttaac
     1021 tttttcagct atgcagggaa aaacggtcat gcctatcgca gcattggtaa ggtgttgatc
     1081 gaccgtggcg aagtgaaaaa agaagatatg tcgatgcagg cgattcgtca ctggggcgaa
     1141 acacacagtg aagcggaggt tcgcgagctg ctggaacaga acccgtcttt cgtcttcttt
     1201 aaaccgcaat cttttgcacc ggtgaaaggg gcaagtgcgg tgccgctggt tggccgtgct
     1261 tctgttgcct ccgatcgttc aattattccg ccaggtacta ccttgctggc agaagtgccg
     1321 ctgctggata ataacggcaa atttaacggt cagtacgaat tgcgtctgat ggtggcgctg
     1381 gatgtcggtg gcgcaatcaa aggccaacac ttcgatatct atcaagggat cgggccggaa
     1441 gccggacacc gcgcaggttg gtacaaccac tatggacgtg tctgggtgct gaaaaccgcc
     1501 ccgggcgcag gtaacgtctt tagcggctga tgtgatattc tgtatgccgt ttttacggat
     1561 atgacagggt gaggggtaac ctcacccttt ttaattctga ggtgctatgt ctgtggtaat
     1621 tagtgatgca tggcgtcagc gttttggtgg cacagcgcgt ctgtatggtg aaaaagcatt
     1681 gcaactgttt gctgacgcgc atatttgtgt ggttggtatc ggtggtgtcg gttcctgggc
     1741 ggcggaagcg ctagcgcgca cggggattgg cgcaatcacg cttatcgata tggatgatgt
     1801 gtgcgtcacc aataccaatc ggcaaattca cgctctgcgc gataacgtcg ggctggcaaa
     1861 agcggaagtc atggcggagc gtattcgcca gattaacccg gagtgccgtg taatggtggt
     1921 ggatgacttc gtgacgccgg ataacgtagc gcaatatatg aatgccggtt attcgtacgt
     1981 gattgatgct attgatagtg tacggcccaa agcggcgctg attgcttatt gtcggcgcaa
     2041 taaaatcccg ctggtcacga ccggtggcgc tggtgggcag attgacccga cgcagattca
     2101 ggttaccgat ctggcgaaaa cgattcagga cccgttggcg gcgaagttgc gcgagcgcct
     2161 gaaaagcgat tttggcgtag tgaaaaacag taaaggtaag ctcggcgtgg attgcgtgtt
     2221 ctctaccgaa gcgctggtgt acccgcagtc agacggcacc gtctgtgcga tgaaagccac
     2281 ggcggaaggg ccaaagcgga tggattgtgc ctcaggattt ggcgcggcga cgatggtgac
     2341 cgccactttt ggctttgttg cggtttctca tgcgctgaag aagatgatgg cgaaagcggc
     2401 gcgtcagggg taaacaatca ggtcggatgc gacgcgaacg tcttatccga cctggttctg
     2461 tgcttaaacc tgcttcgccg cagcgataat cgcctcgctt aacgcattca acccctggct
     2521 gcgtgaggcg ctaagttgcg cacgtaatcc cagctcatca aacaatgcca gcggcgactg
     2581 tgcctgcaat tcggcggtgg ttttcccctc aacggcagtc aacaacaccg ccaacaggcc
     2641 gcgcacaatg cgcccttcgc tgtcgccaaa gaaatgcatc ttgccgtttt cagccacggt
     2701 atatcccagc cagacgcggt tttcgcatcc ggcaatctct ttagcctgcg cttttaactc
     2761 ctctggcaaa gccggaagct gtttccccag catgatcaac tggcgatatt tatcttccca
     2821 ttgcgtcaac ggtgcgaagg tattgcgtaa cgtttctgcg gttacggttg tgccgaacgg
     2881 atgtccggcg aattgcgggt ttgtcattaa tccaccaata attccagcgc gcggtcaacg
     2941 gcattcacca gcgcatccac atcactcttt gtattatatg gcgcaaatga ggcgcgcagt
     3001 gtgccggtta cgcctaattc tgccagtagc ggctgagcgc aatgctgccc ggcgcgcagg
     3061 gcaataccgt actccgccag cagtgtcacc atatcgctgt gatgaacacc cgcaaaatca
     3121 aaggccagca ggctggaatc ctggcagcgg aatgaacgaa agcctggacg tttcgccagc
     3181 gcttcttctg caagcgttgc taagctacgg ctccagcttt cggcctggtt gatatcgtaa
     3241 tctgtcagcc attccagcgc cgcgcttaat cctatgacgc cagcgacatt tggcgttcct
     3301 gcttccagtt tccacggcgc agattgagtc gtgaagccgt caaaactcac ttcgtgaacc
     3361 attttgccgc cgcccagcca gggcgacatc gcttccagca gttctggttt gccatacagc
     3421 acgccgatgc ctgtcgggcc atacagtttg tgacctgaaa aagcatagaa atcaatatcc
     3481 agttgctgaa catccgcggg gaaatgcacc gccccctgag caccatcaac cataaccacc
     3541 atcccggctg aatgagcaaa agtaatcgct ctcgccagat ccgggcaacc gccagtgacg
     3601 ttcgacatct ggcccaacgc cagaatccga ctacggggag taatcagttc tggcaacaaa
     3661 tcgacatccg gcagtcgctg cgcattaagc ggcaatttca ccactttggc ccccgtttgt
     3721 tgggcgacca tcagccaggg gacgaggttg gcgtggtgtt ctgccacact gacaataatt
     3781 tcatcgcccg gttgcagacg cggacgggca tagcattgcg ccaccatgtt gatggattca
     3841 gtggtgccgc gcgtccagac gatatttttg tcatccgatg cattcagtaa ttgtgccact
     3901 ttttcccgcg cagcttcgta acgcgcggtc aggcgttggg cttcggcaaa ctggctgcga
     3961 tggacgtttc cggcgctcag gctataaaac tgtcgggtag cttcaaccac ggcttcaggt
     4021 ttaagcgcgg tcgcggcgct gtcgagatag acgcccgcat cctgtagcgc gggaaactgg
     4081 gcgcgaaact gcgcgggatt aaaaacgttc atggtactcc tcggcttgat gcaccgatcg
     4141 tgacgcaaaa ttcactttga tacaaggaga gtgctaacca tcagaattat ctggatatcc
     4201 tggcgaagcc gcgtcagtaa catatgctga atagtgttag gcgttaattt tagcctgtta
     4261 atcaatacta ttaagattta ttaatcgctt taaggagaag atgatgaaaa agactgccgc
     4321 aattatttct gcctgtatgc tgacttttgc cctgagcgcc tgttccggtt cgaactatgt
     4381 gatgcacacc aatgacggac gtaccatcgt ctctgacggc aaaccacaga ctgataacga
     4441 taccggtatg atttcgtata aagacgctaa tggcaacaaa cagcagatca accgtactga
     4501 cgtgaaagag atggtcgaac tggatcagta attcgcgatc gcaaggtaaa aaaaagcacc
     4561 gcaattaggc ggtgctacat taatcactat ggacagacag ggtaaatgta caggaagtga
     4621 aaaaaggtag ctttgctacc atggtctgaa tcgcagacca attgcaaaca caacaacaca
     4681 acatcacaac cgtaagccaa aagttcacca gaacacgcat tccgataaaa cttttcgttc
     4741 cggctcagga agtgccgcca ctataggtat ttgctggtag aagctcaacg gacaatttat
     4801 aatggctcag attaaaaaaa cta
//
LOCUS       DAZFRG010000309         4816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFRG010000309 DAZFRG010000000
VERSION     DAZFRG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.311"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..836
                     /gene="ydiV"
                     /locus_tag="ACHKUC_004553"
     CDS             123..836
                     /gene="ydiV"
                     /locus_tag="ACHKUC_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO6620667.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPHVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            899..2335
                     /gene="selO"
                     /locus_tag="ACHKUC_004554"
     CDS             899..2335
                     /gene="selO"
                     /locus_tag="ACHKUC_004554"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO6620668.1"
                     /translation="MTLSFITRWCDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGIWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGVNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDSERQQLMQSVNPALVLRNWLAQRAIEAAEKDDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2339..2530)
                     /gene="hemP"
                     /locus_tag="ACHKUC_004555"
     CDS             complement(2339..2530)
                     /gene="hemP"
                     /locus_tag="ACHKUC_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO6620669.1"
                     /translation="MRYTDSRKLTPETDASHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2662..3708)
                     /gene="aroH"
                     /locus_tag="ACHKUC_004556"
     CDS             complement(2662..3708)
                     /gene="aroH"
                     /locus_tag="ACHKUC_004556"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO6620670.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3865..4698)
                     /gene="ppsR"
                     /locus_tag="ACHKUC_004557"
     CDS             complement(3865..4698)
                     /gene="ppsR"
                     /locus_tag="ACHKUC_004557"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO6620671.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1193 a         1134 c         1228 g         1261 t
ORIGIN      
        1 acccctgaaa aagtggttaa catggaacaa atgaaagtta ctccttctgt aattgttatg
       61 tcttatggcc tcatcccttt tagccggata ctgaaaaaca tccttcgaga gggacggtta
      121 ccatgaagat ttttttggag aatctttatc attctgattg ctatttcctg ccgataagag
      181 ataaccagca agaccttgtc ggcgttgagt taattaccca tttctccagt gaagatggta
      241 cggttcgtat cccgacgagt cgggttatag cgcaacttac cgaggagcaa cattggcaat
      301 tattttctga acagttggaa ttactaaaat catgccaaca cttttttatt caacataaac
      361 tctttgcctg gcttaattta acgccacacg tggctacatt attattagac agggataatt
      421 tcgcaggcga actattaaaa tatccgttta ttgaactgtt gattaatgag aattacccgc
      481 atttcaatga aggaaaagat aatcgcgatc ttttatcctt atcgcaaatg tatccccttg
      541 tgttaggtaa tttgggagca ggtaatagca caatgaaagc ggtttttgat ggtcttttca
      601 cccgcgtcat gctggataaa agttttattc agcaacaaat cacgcatcgt tccttcgaac
      661 cgtttattcg tgcgatccag gcgcaaattt ccccttgttg caattgcatc atcgcggggg
      721 gcattgatac gccagaaata ttggcacaga ttatcccctt tgatttccat gctctccagg
      781 gatgcctgtg gcctgcggtt cctataaatc agataacgac gttggttcag cgataaccct
      841 cctgtttgct ggtgtttagg acgagagtaa ccgtctacac tatcaaacag gaggatctat
      901 gaccctgtct tttattaccc gctggtgcga tgaattgcca gaaacctata cagcactttc
      961 ccctacgcct ttaaataatg cccggctgat ttggcataat accgaactcg ctaacacgct
     1021 gagtattcca tcgtcgctgt ttaaaaatgg cgcaggtgtc tggggcggcg aaaccctact
     1081 gcctggtatg tcaccactgg cgcaggttta cagtggtcat cagttcggca tctgggcggg
     1141 ccaactgggt gatgggcgcg gcattttact cggcgaacaa ctgcttgctg atggcactac
     1201 aatggactgg catctgaaag gtgctggcct gacgccttat tcgcgaatgg gtgatggacg
     1261 ggcggtttta cgttcgacga tacgagaaag tctcgccagt gaggcgatgc attatctggg
     1321 cattccgacg acccgcgcgt taagtatcgt caccagcgat tcgccagtgt atcgggaaac
     1381 ggtggagtca ggcgcgatgc tgatgcgtgt ggcaccaagt catctgcgct ttggtcattt
     1441 cgaacatttt tactatcgcc gcgagccgga aaaggttcgt cagttagctg actttgccat
     1501 tcgtcattac tggtcacatc ttgatgatga ggaggacaaa taccgtctct ggtttaccga
     1561 tgttgtcgca cgtaccgcct cgttaattgc ccaatggcag acggtcggct ttgctcatgg
     1621 agtgatgaat accgacaaca tgtcgctgct ggggctgacg cttgattacg ggccgtttgg
     1681 ttttcttgat gattacgaac ccggttttat ttgtaatcac tcggatcatc aagggcgtta
     1741 cagctttgat aatcaacctg ccgtcgcgtt gtggaattta cagcgtctgg cgcagacatt
     1801 gtcgccattt gttgccgtag atggcgtgaa tgaggccctg gacagctatc agcaggtttt
     1861 gttgacgcat tatggacaac ggatgcggca gaaactgggc ttcatgacgg agcaaaaaga
     1921 ggataacgcg ctactgaatg aattattcag tctgatggcg cgagagcgca gcgattatac
     1981 ccgcacattc cgcatgctga gtctgaccga gcagcacagc gcggcgtcac cgctacgtga
     2041 tgagtttatt gatcgtgcgg catttgatga ctggtttgcc cgttatcggg ggcgtttgca
     2101 acaagacgag attaccgata gtgagcgtca gcaactgatg caaagcgtta acccggctct
     2161 ggtgttgcgc aactggttgg cgcaacgggc gattgaagcg gcagaaaagg atgatatgac
     2221 ggaattgcac cgcctgcatg aggcgttgcg aaatcctttc agcgacagag atgatgacta
     2281 tgtcagtcgt ccacccgact ggggtaaacg gctggaagtc agctgctcga gttaaaggct
     2341 acttggtcaa cagcagcttg ccagcctgag ttttacggag cagatattct tgcccgtcat
     2401 gatcgataat cagtttgcca tccggaccta acagtgtctg gctggtaatt cgccggatgg
     2461 gctgcgggga agcggtcttg tgactggcat ccgtttcagg cgtgagtttt ctgctatccg
     2521 tataacgcat aaaaattaat aataacttat ataacaatga ttcttattat caatattatg
     2581 gcgggccaga gcaagcagta atttgttgag aagacaacaa gttgcgcaga aatgcccatc
     2641 ccgtcaggaa tgggcacgca ttcagaagcg agtatctacc gcagaggcga gtttttcgac
     2701 caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac cgtaagtgag
     2761 cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg aaactttccg ccataattcc
     2821 agcaatcgcc gtagagccat tgcggatttg ctgacaaata tcctcacaaa cttctaactg
     2881 gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat gttcaggtaa
     2941 atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat aattcggttt
     3001 tttgccacca cgcataataa tgtggccata cgggttgccg ctggtctgat agatggtcat
     3061 ctgaccattt ttgtctggcg agaggaacat atggctggcg cgggctgcgc ggatagcatc
     3121 cacggcaatc cgcgtgttgc catcggtacc atttttaaaa cccaccggac aggagagtgc
     3181 cgaagccatt tcgcggtgga tctgactttc ggtagtacgt gcgccaatcg caccccaact
     3241 gattaaatca gcaataaact gaccggtcac catatcgagg aactcagtcg cggtagggac
     3301 gcccagctca tttacctgta aaagtaattt gcgcgccagc tccagaccgt gatttacccg
     3361 atagctaccg tttaaatctg gatcggagat gagtcctttc cagccgacaa ccgttcgtgg
     3421 tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt tgcgcagcga
     3481 ctgcagacgg gtggcgtact ccatcgcagc ggtgagatcg tggatcgagc aggggccaat
     3541 aatgaccaac agtcgcttat cttcaccatt cagtattttt tcaattctac ggcgggagtc
     3601 ggtgacatgg gtggcgacgc caggcgttac gggataccgt agcgcgagtt cggcgggcgt
     3661 taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta caagtctcag
     3721 aaatttgttg cttctacggc aggactaccg ggaagtgatg cgcatcacga taaaccaatc
     3781 gcagctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca tgataacaaa
     3841 aaaacaagtt aatgcactag ttctctagta cattcggcgg ctaaggccca tgatatcgag
     3901 gatcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg ggatctgatt
     3961 tttacggtac aacgcttcca cttccgcgac ttccatcctg cactgacgaa gcgaggcata
     4021 gcgactgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt cgatagtcag
     4081 gccgaacaat ttgtgctgaa gtggtttgag cgacgcgggt agcaccagat tatccatatc
     4141 gtcggcaata aaggggtaat ttgccgcgcg gataccaaat tgcattgcca gatacagact
     4201 ggtgggggtt ttaccacagc gagaaacacc gagcaggatc acctgagcct ggtccagatt
     4261 gcgcaacgaa ataccgtcat cgtgggcaag ggtgtaatca atcgccgcaa tgcgcgcatc
     4321 atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg gcgttggatc
     4381 cagtttcatc tcttgttgta gcggggcaac cagcgcctga acgatatcct ggcaaaagcc
     4441 ttcactttgc aagatgatag cgcgaatctc cggcaacacg atggagtaga agaccagcgg
     4501 gcgcacacct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc gtgcacggct
     4561 ctcattttcg acaaacggca gcgtaatgct gctgatagtg acgggaaatt gtgacattac
     4621 tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa aaacgtggcg
     4681 atcaacagca ttatccattt tgttcttccc gtgatgcaga cataattcta tgaaagcata
     4741 aattaaaaac gcacagaagc gtagaacgtt atgtctggtt tataaaatga aacttaaatt
     4801 ttatttttta tgaaaa
//
LOCUS       DAZFRG010000310         4809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFRG010000310 DAZFRG010000000
VERSION     DAZFRG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.312"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            533..1174
                     /locus_tag="ACHKUC_004558"
     CDS             533..1174
                     /locus_tag="ACHKUC_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620672.1"
                     /translation="MNADTWTRLQAFRHALELTSCEASLTAGYDHLKDFPAGCSELAS
                     QTLTDYLTEDGSNLYSCIVGMQWDNGPGRYGHVIADPARDYIDLTLDQFPGYHNRIVA
                     EPVESGGQLAADLNREPAISTADGIVASPSDKVNYITEQKNRQPMVIITECYKLIRMT
                     ARFIPMCQSSQLFQHQVFPRLFIISDVGSCSLSRNTLDRLATDWKVTLSILSL"
     gene            2349..3326
                     /locus_tag="ACHKUC_004559"
     CDS             2349..3326
                     /locus_tag="ACHKUC_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO6620673.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3569..4309)
                     /locus_tag="ACHKUC_004560"
     CDS             complement(3569..4309)
                     /locus_tag="ACHKUC_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6620674.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGLRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4430..4618)
                     /locus_tag="ACHKUC_004561"
     CDS             complement(4430..4618)
                     /locus_tag="ACHKUC_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO6620675.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPHHDWL
                     KTGSQRQNAPLRIWFYPQ"
BASE COUNT         1249 a         1191 c         1173 g         1196 t
ORIGIN      
        1 taagatatca cctcaaatat aaaagataat tcttacaaaa acgttccgaa aacaaaaaat
       61 acagataaac accaattaat tcagagcaat ctattgcgaa gaatcagagt aacagggccc
      121 catgatataa tcatggggcc ctgttgcttt tacagatgta aaaacgacca atcatttaac
      181 atctgttatc ggcaccgccc cggacgggtt cggcgcaaat atggtatggt accagcccgg
      241 caattcacct gccgtgcaat ctgacgaagc agtaccggcg tatgcagata tatcgtccag
      301 acatatctgc cgcattcagg cggtgagtaa agccttctct ctcaaaagag gagcaacagt
      361 tatccacaga ttctggggat aactgcgaaa agaatatgtg ccaggaaagt cggctcccgg
      421 gaaaaagacc gctggcggcg cacctgctgg atacgaaagc tgagctgaaa ggatcccggg
      481 aggacgtccg taaacttcag tccctggccc gtcatgacgg gaaggcgaaa aagtgaatgc
      541 ggacacctgg acccgcctgc aggccttccg acatgctctg gagctgactt cctgtgaggc
      601 ttctctcacc gccgggtatg atcacctgaa ggatttcccg gccggttgca gcgagctggc
      661 cagccagacg ctgactgatt atctgacgga agacggcagc aacctgtact cctgtattgt
      721 cggtatgcag tgggataatg gtcctggcag atacggccac gtcattgccg accctgccag
      781 ggactatatc gacctaaccc ttgatcagtt tccaggctat cataaccgga ttgtggcaga
      841 gcccgttgag tccggcggac agctcgcggc agacctcaac agggagcctg ccatcagtac
      901 agctgatgga atagtggcca gcccatcaga taaggttaat tacattactg aacagaaaaa
      961 tcgccagcca atggtgatca ttacagaatg ttacaagctg atcagaatga cggcaagatt
     1021 tataccaatg tgccagtcat ctcaactctt ccagcaccag gtattccctc gcttatttat
     1081 catttcagac gttggctcct gcagtttgtc caggaacacc ttagatcgtt tagctactga
     1141 ctggaaggtg acactgtcca tcctgtcgct atagggcttg atataaaact ggcaggcaag
     1201 ggatctggtt aactcctttc cttcaataaa gtgtttgaag atccagagat acgtatccac
     1261 aagcaccagc cctgtactct gtgtccccga ctggaagacc tgcggttcag ctggtatttc
     1321 cattacatcc atgaccggtg tctgcgcagc agtgcctgtc atcaggatcc tgtcactctt
     1381 tcgaccgaca gattatggca tacagttggg ctcgtaagat ccagagtcaa taactgacag
     1441 ggtttattct gtcatcctcc ggcagcaaag gcatatacga tattcactta ttttgtcggt
     1501 ccaacggctt tttacagtag ctcgttttgc ccaactgatg ctacagctct ttggtttgct
     1561 tcatcacgga tatcagctcc gacacaggca cagctgactg gttgcaacgt ctgcacttct
     1621 ctggacctga aacgtcaatt atcaggattc tgacgattag tcttaagact gtcggtaaat
     1681 taagtgatgc cagttgtcag gatattcatg gagccagtct gcatgcgcct ctggcgttga
     1741 gacaaacata tgaagtgtat ttcggatatg actgtacgct caggaagtac ggcagattcc
     1801 gtcaaaaatt acctgtaaag acagacttca gaaaaaacag tgctcgccgc agaccggtga
     1861 ccgggcgaag cccgcgaacc ggcgaggaag cgcaatatat ctgtagttac agtgagcagg
     1921 tacgccctga agccttcata atcggtagtg tatgttgttt ttctggaatg atttatccac
     1981 atatccagag gcccgatcca gtaaacagat ccatgaatga tcaacaaagg atccattaaa
     2041 gatccccata tcgctgcaaa ccttgtcact catgggccgg gaccacgttc acataagctg
     2101 tggcatgtta tggataaact gtaacaggtt catgataagc tgtattcagt aatgcatata
     2161 ctgaagtaag ttaatgacat aaactatggt cagtacgcca gactcagttg ttaaatacag
     2221 actgcaggtt tttcttcagt cagttgacgg ggttctgaca cacgattttc tgtttattct
     2281 tttactgtcc acaggcagga ggctttctgg aaaacgaaaa ttcagacatc aaaaaactgt
     2341 tcggcgaggt ggataagtcg tccggtgagc tggtgacact gacaccaaac aataacaaca
     2401 ccgtacaacc tgtggcgctg atgcgtctgg gcgtctttgt acctaccctt aagtcactga
     2461 agaacagtaa aaaaaataca ctgtcacgca ccgatgccac ggaagaactg acgcgtcttt
     2521 ctctggcccg tgcagaagga ttcgataagg ttgagatcac cggcccccgg ctggatatgg
     2581 ataacgattt caagacctgg gtggggatca ttcattcctt tgcccgccat aacgtgattg
     2641 gtgacaaagt tgaactgcct tttgtcgagt ttgcaaaact gtgtggtata ccctcaagcc
     2701 agtcatcacg caggctgcgt gagcgcatca gcccttccct gaaacgcatt gccggtaccg
     2761 tgatctcctt ttcccgcacc gatgagaagc acactcggga atacatcacc catctggtac
     2821 agtcagccta ctacgatact gaacgggata ttgttcagtt acaggctgat ccccgccttt
     2881 ttgaactgta ccagtttgac agaaaggtcc ttcttcagct taaggcgatt aatgccctga
     2941 agcgacggga gtccgcccag gcactttaca cctttataga gagcctgccc cgggatccgg
     3001 caccgatatc gctggcgcgg ctgcgtgcac gcctcaatct gaagtctcct gtattttccc
     3061 agaaccagac ggtcagacgg gcaatggagc agttgcgcga gattggatat cttgattaca
     3121 cggagatcca gcgagggcgg acaaaattct tctgtattca ctaccggcgt ccccggttaa
     3181 aagcgccgaa tgatgagagt aaggaaaatc agttgccacc ttcacctgcg gaaaaaatca
     3241 gtccggagat ggcggagaag cttgccctgc ttgaaaaact gggcatcacg ctggatgacc
     3301 tggaaaaact cttcaaatcc cgctgaacat aaactgtagt cagataagag agactcttta
     3361 ctgactacag tttatattaa caggtgcagt gagtgatgtg ctcactgtag tttatattca
     3421 gtttcctgct gcactgtctg tggctgagct gccatctgcc tgttccttac ataaaacacg
     3481 gcttactcac tacagtttat ctacctgcta tagcttatgt tatgcctgtt ctgcttacct
     3541 ccgcttatat aaggaagagc gtatgctgtc acttcagggg aggcagtgta cgcaggatct
     3601 ctgcagcatc gcgcccgtca cctgtgaaag gcactgccag cgtggcagcc atatccagcg
     3661 caaaaactct ggtataaacc tccatagaac gtggatccct gtgaccagcc agtgcctgaa
     3721 tgactttccg gggctggcgg tgataaagca tgtgcatgat atagctgtgc cggaaggtgt
     3781 gtggtgtaac cggaatcgaa aagtgaaccc cgtctgcttc ggcccgtctg acagcctgct
     3841 tcagccagtt gcgcatggtt tcgtcggtca cggcccataa tggttcacga cgccggggcc
     3901 gggtggtgat catccagctt tccatctgcc tgacatagct tatatctgtc agcggaacca
     3961 ggcggacttc atcttttggc gggcgtccgc gtcgcgcacg cactttttcg gacaggatcc
     4021 gcacaaacgg tcgcagacca tccagatcaa atgattccgg cgtcagcatc cgggcttcgc
     4081 caatacgcat tccggtattc cagagggtgg caaacagcat atggtgacgc tgatccggca
     4141 tatagaagag aagggcactg acttccggag ccagcaggta ggccggtgtg gcccccgtgg
     4201 aagtggccat tcttctcagg gagagcgctg ttgcaaaatc cacccccggc gcaatgggta
     4261 acagggagac gcgttctggt gaattctgca ggggaatgac attgttcatg agttattttt
     4321 cacgtagcac acgcactgtt tatggataca ggtattctga cctgaacgcc cggatccttc
     4381 cacgacaata aatcgcagcg cgcggatcct tttgtttgat tatcacgcat tattgcgggt
     4441 aaaaccaaat acgtaaaggc gcattttgcc gctgggatcc cgtcttaagc cagtcatggt
     4501 gcgggctgag ggctggttta ccgtttttgg gagaattggg gtaatgccag gaacaggcag
     4561 atacggagaa aaatcctgca gataaccttc tacgacaact gaaggtgtct gacgccatat
     4621 tcttagctac ttctcagttt taaggcggct tagtcacaac ataaggttat gcataataag
     4681 catattttat tatgttatgg tcttgttcat gcatgaactg ttatctattc agtcactttt
     4741 aacgttttat atcatactgc atatgacaca atcatcttct atattcagat taaaaatatt
     4801 atgacgacg
//
LOCUS       DAZFRG010000311         4786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFRG010000311 DAZFRG010000000
VERSION     DAZFRG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.313"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1971
                     /gene="yihQ"
                     /locus_tag="ACHKUC_004562"
     CDS             <1..1971
                     /gene="yihQ"
                     /locus_tag="ACHKUC_004562"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO6620676.1"
                     /translation="RFQDRLILIHSKDNPCLWIGSGIADIDMFRGNFSIKDKLQEKIA
                     LTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGRLLLELQNDNLNHNRIWLRLAAQ
                     PEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYWTFF
                     PQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEDKATLRFECADTYISLLEKLT
                     ALLGRQTELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSF
                     GKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYINPYVASDKDLCEEAAKRGYLAK
                     DAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCGGWMADFGEYLPTDT
                     YLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEILFFMRAGSTGSQKYSTMMWAGD
                     QNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAF
                     TPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFTTLKPYLKEAVALNAKSGLPVMR
                     PLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWTGEAFRG
                     GEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2017..3402
                     /locus_tag="ACHKUC_004563"
     CDS             2017..3402
                     /locus_tag="ACHKUC_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620677.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3445..>4786
                     /locus_tag="ACHKUC_004564"
     CDS             3445..>4786
                     /locus_tag="ACHKUC_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620678.1"
                     /translation="MSNHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDATP
                     TGHKASILKSFYAIFSNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEE"
BASE COUNT         1093 a         1198 c         1233 g         1262 t
ORIGIN      
        1 cgttttcaag accgccttat tttaatccat agcaaagata atccttgttt atggattggt
       61 tcaggtatag cggatatcga tatgttccgc ggtaatttca gcattaaaga taaattgcag
      121 gagaaaattg cgcttaccga cgccatcgtc agccagtcac cggatggctg gttaatccat
      181 ttcagccgtg gttctgacat tagcgccacg ctgaatatct ctgccgacga tcatggccgt
      241 ttattgctgg aactacaaaa cgacaacctt aaccacaacc gcatctggct gcgccttgcc
      301 gcacagccgg aagatcatat ctacggctgc ggcgaacaat tctcctactt tgatctgcgc
      361 ggcaaaccgt tcccgctgtg gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat
      421 gtcacctggc aggccgactg caaagagaat gcgggagggg attactactg gaccttcttt
      481 ccacaaccca cgtttgttag cacgcagaag tattactgcc atgttgataa cagttgctat
      541 atgaacttcg actttagcgc gccggaatac catgaactgg cgctgtggga agacaaagca
      601 acactgcgtt ttgaatgtgc tgatacatac atctccctgc tggaaaaatt aaccgcgctg
      661 ctgggacgcc agacagaact gcccgactgg atttatgacg gagtaacgct cggcattcag
      721 ggcggcactg aggtgtgcca gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtc
      781 aacggcatct gggcgcagga ttggtccggt attcgtatga cctcttttgg caaacgcgta
      841 atgtggaact ggaagtggaa cagcgaaaac tacccgcaac tggattcacg tatcaaacag
      901 tggaacaaag agggcgtaca gttccttgcc tatatcaacc cgtatgttgc cagcgataaa
      961 gacctctgcg aagaagcggc aaagcgcggc tatctggcaa aagatgccgc cggcggcgat
     1021 tatctggtgg agtttggcga gttctacggc ggcgttgtcg atctcactaa tccggaagcc
     1081 tacgcctggt tcaaggaagt gatcaaaaag aacatgattg aactcggctg cggcggctgg
     1141 atggctgact tcggcgagta tctgcccacc gacacttacc tgcataacgg cgtcagcgcc
     1201 gaaattatgc ataacgcctg gcctgcgctg tgggcgaaat gtaactacga agcccttgaa
     1261 gaaacgggca agctcggcga gatcctcttc tttatgcgcg ccggttccac cggtagccag
     1321 aaatactcca ccatgatgtg ggcgggcgac cagaacgttg actggagtct ggacgatgga
     1381 ctggcgtctg tcgtcccggc ggcgctgtcg ctggcaatga ccggacatgg cctgcatcac
     1441 agtgatattg gcggttacac caccctgttt gagatgaagc gcagcaaaga gctgctgctg
     1501 cgctggtgcg atttcagcgc cttcacgccg atgatgcgca cccatgaagg taaccgtcct
     1561 ggcgacaact ggcagtttga cggcgacgcg gaaactatcg cccattttgc ccgtatgacc
     1621 accgtcttca ctaccctgaa accttacctg aaagaggccg tcgcactgaa tgcgaagtcc
     1681 ggcctgccgg ttatgcgccc tctgttcctg cattacgaag acgatgcaca gacctacacc
     1741 ctgaaatatc agtacctgtt aggccgcgac attctggtcg ccccggtaca cgaagaaggt
     1801 cgcagcgact ggacgctcta tctgccggag gataactggg tccatgcctg gacgggtgaa
     1861 gcgttccggg gcggggaagt taccgttaat gcgcccatcg gtaaaccgcc ggtcttttat
     1921 cgcgccgata gcgaatgggc ggcactgttc gcgtcattaa aaagcatcta atcacatccg
     1981 cccgtgggga aactcacggg caaagggaga acaacgatga gtcacatcac aacggaagat
     2041 ccggctactt tacgcctgcc ctttaaagag aaactctctt acggtatcgg cgatctggcc
     2101 tctaacatcc tactggatat cggcacgctt tatcttttga agttttatac cgatgttctg
     2161 gggctgcctg gcacctatgg cggcattatc tttttgattt caaaattctt taccgccttt
     2221 accgatatgg gtaccggcat tatgttggat tcccgacgca agatcggtcc aaaaggtaag
     2281 ttccgtcctt ttattctgta tgcgtcattt ccggtcaccc tgctggcgat cgccaacttt
     2341 gtcggcacac cgtttgatgt caccggtaaa acggtgatgg ccaccatcct gtttatgctt
     2401 tacggactgt ttttcagcat gatgaactgc tcgtatggcg cgatggtccc cgctattact
     2461 aaaaacccca acgaacgtgc atcgctggca gcatggcgtc agggtggcgc tacattgggc
     2521 ctgctgctgt gtacggtggg attcgtgccg gttatgaatc ttatcgaggg taatcagcaa
     2581 cttggctata tcttcgccgc cacgctgttt tcactgttcg gcctgctgtt tatgtggatc
     2641 tgctattcgg gcgtgaaaga gcgttatgtc gaaacccaac ctaccaatcc ggcgcaaaag
     2701 cctggcttgt tgcaatcttt ccgcgcaatt gcggggaacc gcccactgtt cattttgtgc
     2761 attgccaacc tctgtacctt aggggcgttt aacgtcaagc tcgccattca ggtctattac
     2821 acccagtacg tgcttaacga tcccatcctg ttgtcatata tgggattttt cagcatgggc
     2881 tgtattttca tcggcgtgtt cctgatgcct ggtgcagtca gacgttttgg taagaagaag
     2941 gtctatatcg gcggtctgct gatctgggtg ctgggcgatc tgctcaacta tttcttcggc
     3001 ggcggctcgg tcagctttgt ggctttctcc tgcctggcgt tctttggttc ggcgtttgtt
     3061 aacagcctga actgggcgct ggtttctgac accgtcgagt atggcgagtg gcgcaccggt
     3121 gtgcgttcgg aaggtacggt ctacaccggt ttcaccttct ttcgcaaagt atctcaggcg
     3181 ctggcaggtt tcttcccagg ctggatgctg acgcaaattg gctatgtgcc aaacgtcgcc
     3241 caggctgacc acactatcga agggttgcgc caactgatct tcatctaccc aagcgcactg
     3301 gcagtagtca ccattgtggc aatgggttgc ttctacagtc tgaacgagaa gatgtacgtc
     3361 cgcattgttg aagagataga agcccgtaaa cgcacggcgt aattataata aacaacgccc
     3421 tgcggggcgt tataaggagt gattatgtct aaccataatc cactgacatt aaaactgaat
     3481 ctgcgggaaa aaatcgccta tggtatgggc gacgtcggtt cgaatttaat gcttagcatc
     3541 ggcactctgt atctcctcaa attttatacc gatgaattag ggatgcctgc ttactatggc
     3601 gggattatct ttctggtggc gaaattcttt accgcattta ccgatatgct caccggattt
     3661 ttactcgact cgcggaaaaa tattggacct aaaggaaaat tcaggccgtt tattttatac
     3721 gcagcgattc cggcggcgtt aattgcaacg ctccagttta tcgccaccac ttttagtttg
     3781 ccggttaaaa cgacgattgc caccgcgctg tttatgatgt ttggactttc atacagtctg
     3841 atgaactgct cgtatggcgc gatgatcccg gccattacca aaaacccgaa tgagcgcgcg
     3901 caactcgctg cttaccgcca gggtggtgca accatagggc tattgatctg taccgtggcg
     3961 tttattcctt tgcagtcact attttctgac tccaccgtcg gctacgcctg cgcggcactg
     4021 atgttctcca ttggcggctt tatttttatg atgctgtgct acagaggtgt caaagagcat
     4081 tatgtggacg cgacgccaac cggacataag gccagtattc tcaaatcatt ttacgcaata
     4141 tttagtaatc cacctttgct ggtattgtgc attgctaacc tgtgtaccct ggcggcattt
     4201 aatatcaaac tggcgattca ggtctattac acccagtatg tgctgaatga tattaactta
     4261 ttgtcgtgga tgggattctt tagtatggga tgcattctcg tcggcgtatt actggtgcca
     4321 ctaactgtaa aatgttttgg taaaaaacag gtttatctgg ctggcatggt gctgtgggcg
     4381 gtgggggata tcctgaatta ttgctggggc agtaactcat tcactttcgt catgttctct
     4441 tgtgtcgcct tttttggcac ggcctttgta aacagcctga actgggcact ggtaccagat
     4501 accgtagatt atggtgaatg gaaaacaggt atacgtgccg aaggttctgt ttataccggt
     4561 tataccttct ttcgtaaaat ttcagccgca cttgctggct tcttgccagg cattatgctg
     4621 acgcaaatcg gttatgttcc caacatcgcg caaagcgatg caacattaca gggtttgcgt
     4681 cagctcattt ttatctggcc ttgtgcgctg gcaattattg ctgcattgac gatggggttc
     4741 ttttacacgc tcaatgagaa acggtttgca ctgattattg aggaaa
//
LOCUS       DAZFRG010000312         4764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFRG010000312 DAZFRG010000000
VERSION     DAZFRG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.314"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..1123
                     /gene="hemB"
                     /locus_tag="ACHKUC_004565"
     CDS             149..1123
                     /gene="hemB"
                     /locus_tag="ACHKUC_004565"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO6620679.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1163..2014)
                     /gene="tauD"
                     /locus_tag="ACHKUC_004566"
     CDS             complement(1163..2014)
                     /gene="tauD"
                     /locus_tag="ACHKUC_004566"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO6620680.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAHRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(2011..2838)
                     /gene="tauC"
                     /locus_tag="ACHKUC_004567"
     CDS             complement(2011..2838)
                     /gene="tauC"
                     /locus_tag="ACHKUC_004567"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO6620681.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILLTVWWVVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2835..3602)
                     /gene="tauB"
                     /locus_tag="ACHKUC_004568"
     CDS             complement(2835..3602)
                     /gene="tauB"
                     /locus_tag="ACHKUC_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO6620682.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3615..4577)
                     /gene="tauA"
                     /locus_tag="ACHKUC_004569"
     CDS             complement(3615..4577)
                     /gene="tauA"
                     /locus_tag="ACHKUC_004569"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6620683.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDIPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1101 a         1372 c         1216 g         1075 t
ORIGIN      
        1 aatatgaaat tattgctatt tctattacaa agatgtcctg acctcttttt cattctcttt
       61 ttgtcaacat cgcgacaact ttcgtaaaac acacccagcc tgcttcaggt atactatgct
      121 cctcgattcc acaaacatca ggcagaccat gacagattta atccaacgcc ctcgtcgcct
      181 gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc ttaacgacct
      241 ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg aagccatgcc
      301 aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca tcgccaacgc
      361 cggtattcgt tccgtgatga ctttcggcat ctctcaccat accgatgaaa ccggcagcga
      421 tgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc agaccgtgcc
      481 agaaatgatc gttatgtcag acacctgctt ctgtgaatac acatctcacg gtcactgcgg
      541 tgttctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag gcaagcaagc
      601 tgtggttgca gctgctgcag gcgcagactt catcgcccct tctgccgcga tggatggtca
      661 ggtacaggcg attcgccagg cgctggacgc tgcgggcttt aaagatacag cgattatgtc
      721 gtattcgacc aagttcgcct cctcctttta tggcccgttc cgtgaagctg ccggaagcgc
      781 attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgtg aggcgattcg
      841 tgagtcactg ctggatgaag cccagggcgc agactgcctg atggttaaac ctgccggagc
      901 atacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg gcgcgtatca
      961 ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga
     1021 gaaagtcgtg ctcgaaagct taggttcgat taagcgtgcg ggtgcggatc tgattttcag
     1081 ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taatgatcat tatgccgggt
     1141 tatccaaccc ggcatttccc ccttaccccg cccgataaaa cggaatatcc ccaagaatcg
     1201 tcgcccgatg cataatccgc cgctgtggca ggtaatccgc attggcatag tgctgagtca
     1261 cgcggttatc ccaaatcgca atatcattcg gttgccagcg ccagcgcacc tgaaactccg
     1321 gtttagtgat atgggcaaac aaaaaaccta acaaggcttc gctctctttc tcgctgacat
     1381 caacaattcg tgtggtaaaa ccttcgttca caaacagcgc ctgtttaccg gtcaccggat
     1441 gcgttcgcac caccggatgt agcaacggtg gatttttcgc gaccgcctca cgccagcgtt
     1501 gatgctcagc ctcggttttg cggtacttgt attccgggaa cgatttacgg aaatcatgct
     1561 ccgcacgcaa cccgctcaac agctggcgga agggaacaga gagcgcctca tatgcggcaa
     1621 taccactggc ccagagcgta tcgccgccgg tcgagggtaa ctctttcgca gccagaatcg
     1681 cccctgcggg tggcgtttga ataaatgtca catcggtgtg ccagttgtcg ttatccggcg
     1741 gattatcgtt atgggtatcc aggacgatga tctcgtcaac cccttcagca tgagggtaaa
     1801 cagggtgaat atgcagctcg ccaaaacgat gggccagcgc gcgttgctgc tgcggcgtaa
     1861 tagcttgatc gcgcagaaac accacctgat ggcgcagcac cgcatggtaa agctgttcaa
     1921 actgattatc gcttaacggg cgcgttaagt cggcccccgt aatttgtgcg ccaatatacg
     1981 gccccagcgg ggtaatgctc agacgttcac tcattgtact tctccatgcc agggcgtcag
     2041 gcggcgctgt aacgcgcgca gacccagttc taaaagaaag gcgataatcg caatcaccgc
     2101 gatccccgcc agcaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc
     2161 taaaccgcgc gttgcggcaa tcagctccgc cgccaccagc gtagaccagc ccacacccag
     2221 accaatgcgt aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag
     2281 cacctgcgca cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct
     2341 tttcaccccc gccagcgccg acatcgccac cggcgcaaaa atcgctagat agatcagtaa
     2401 gatcttcgat gtttcgccaa taccaaacca aatcaccatc agcggcaaat aagccagcgg
     2461 cggcaccgga cgataaagct cgattatcgg atccagaatg ccgcgcaccg tcgggcttag
     2521 ccccatggcg atcccgaccg gaataccgat caacaccgcc gcgagtagcg ccagcaaaat
     2581 gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggccc
     2641 ggcaatggtg agcagttttg ccagtacctg ttgcggtggc ggcaaaaaca gcgggctaat
     2701 cagttgcagc gccgcgacta cccaccatac agtgagtaaa atcgctaacg tgccaatgct
     2761 taaggtcacc tgacgcgaga gcggccagcg ccatttcagc cgatgcgaat gcagtttttc
     2821 attaatgagc acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat
     2881 tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca
     2941 aagcggcggg cgaagttgag cggcagccgc tccagcacgc ggcctgggcc gggtgaaagc
     3001 agaaccagtt cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc
     3061 tgctttcccg tctcctgcca aagtttcagc agcagggttt gcatctggtc gcgggtgaag
     3121 gcgtccagcg caccaaacgg ttcgtcgagt aataacagct ggggatttgc cgccaacgcg
     3181 cgggcaatcc ccacccgctg acgttgacca ccggaaagct gccagatgta gcgtttttct
     3241 gcgccttcca gcccgacttt tttcaccatc tggtgtgcga tttccagtcg ctgcattttc
     3301 tctacacctg ccagttgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtaac
     3361 agcccttcat tctgaaaaac tacgccacgc tctgctcccg gtccctgaat acccttaccc
     3421 gccagttgaa tgctgccatg ctgataaggc acaaaaccag caatcaaatt cagcaacgta
     3481 gttttgccgc agccagacgg ccccagaact accagaagct cgccgctttc cagcgtcagg
     3541 ttgatatctt ccagtgccgg tttgccgcca taatcggcgt aaagatgaga aatttgcagc
     3601 atctgcgcct ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct
     3661 acagccggga ctttgccctg ctctttcaaa aactgcgcgg tgtcgatgat cgctttgttc
     3721 accggtccgg tcagttctgc cgtttgctgc tgcggcgtca gataggtatt ccccttcacc
     3781 agccccggaa tgtcaccttc cggcacgccg cttaaccgcg ccagtttgct gatgttttcc
     3841 ggctgtttca gccacgcgtc tgggttagca atgtacggtt gctgagcatc aatggcgctt
     3901 ttagcgaacg ctttcacgac ctcaggatgt ttctcggcaa aatctttccg cacgacccag
     3961 acgtccagtg tcggcgcgcc ccactgcccg acctgttcag aatcggtcag caccttgcca
     4021 tctttttcca gggcgttaac cgccggtgcc cagacataag caccatcaat atctccccgc
     4081 tgccatgcgg cgataatcgc gggcggctgc aggttcacaa tctcaacttg cccaggttta
     4141 ataccccagt gtttcagcgc cgccagcagg ctgtagtggg tggtggagat aaacggtacg
     4201 gcgatgcgtt taccaatcaa gtcttccggt ttactgatag ttttctttac caccagcgct
     4261 tcggagttac ccagttttga cgccagcaag aagacttcaa tcggcacctg ttggctggtt
     4321 gcaaccgcta acgggctgga accgaggttg ccgatttgca cgtcgcccga agccagcgcc
     4381 cgcacgatgc tggctccgct gtcaaactta cgccagtcca cggttgctcc gctttcttta
     4441 gcaaaggtgt tgtcggcctg agccactttc gccggttcgg cggaggtttg atacgccacg
     4501 gtgacgttca ccgcctgcgc ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta
     4561 cgcgatgaaa ttgccatgat tgtctgctcc cccgtcttgt tatgggagca gtattcagga
     4621 ataaaaacat tcattaaaag aattagtcgt tatcgtacag atgattttat tcttagcaaa
     4681 aaagcggcaa aggaaagatt aaatatgcaa ttacgcccac tctgataact cagtaaatta
     4741 ctgcacttca ttttattaat gcat
//
LOCUS       DAZFRG010000313         4755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFRG010000313 DAZFRG010000000
VERSION     DAZFRG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.315"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1336
                     /gene="garP"
                     /locus_tag="ACHKUC_004570"
     CDS             2..1336
                     /gene="garP"
                     /locus_tag="ACHKUC_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO6620684.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1352..2122
                     /gene="garL"
                     /locus_tag="ACHKUC_004571"
     CDS             1352..2122
                     /gene="garL"
                     /locus_tag="ACHKUC_004571"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO6620685.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGIFRSATQKLADTFKK"
     gene            2152..3042
                     /gene="garR"
                     /locus_tag="ACHKUC_004572"
     CDS             2152..3042
                     /gene="garR"
                     /locus_tag="ACHKUC_004572"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6620686.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGM
                     ADHSALACYYEKLAKVEVTR"
     gene            3139..4284
                     /gene="garK"
                     /locus_tag="ACHKUC_004573"
     CDS             3139..4284
                     /gene="garK"
                     /locus_tag="ACHKUC_004573"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO6620687.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4317..4693
                     /gene="rnpB"
                     /locus_tag="ACHKUC_004574"
     ncRNA           4317..4693
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKUC_004574"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1087 a         1143 c         1387 g         1138 t
ORIGIN      
        1 aatgatgctg gacaccgttg acgaaaaaaa gaaaggcgtg catacccgct atttaatatt
       61 actgattatt tttattgtta ccgccgttaa ctacgccgat cgtgcaacgc tgtctattgc
      121 tggtaccgaa gtggcaaaag agttgcagtt aagtgcggtt tcgatgggtt acatcttctc
      181 tgcttttggc tgggcctact tgctgatgca aattcccggc ggctggctgc ttgataagtt
      241 tggctcgaag aaagtttaca cttacagcct ctttttctgg tcgctgttca ccttcctgca
      301 aggctttgtc gatatgtttc cgctggcctg ggctgggatc tccatgttct ttatgcgctt
      361 tatgctgggg ttctcggaag cgccatcatt cccggcgaac gcccgtattg tcgccgcctg
      421 gttcccgacg aaagaacgtg gtactgcctc cgccatcttt aactcggcgc aatatttctc
      481 gttggcgctc ttttcgccgc tgcttggctg gctgactttc gcctggggct gggagcacgt
      541 ctttaccgtt atgggggtga ttggttttgt gctgacggcg ctgtggatca agttgattca
      601 taacccgaca gatcacccac gtatgtctgc ggaagagctg aagtttatct cggaaaatgg
      661 cgcggtggtc gatatggacc acaaaaagcc gggcagtgcg gcagcaagcg gacccaaact
      721 gcattacatc aagcaattgc tctctaaccg catgatgctg ggcgtatttt tcggacaata
      781 ttttatcaac accatcacct ggttcttcct cacctggttt ccaatttatc tggtgcagga
      841 aaaaggcatg tcgattctga aagtgggtct ggtcgcctcg attccggcac tgtgtggctt
      901 tgcgggcggc gtgctgggag gtgtcttctc ggattatctg atcaaacgcg gtttgtccct
      961 gaccctggca cgtaagctac caattgtgct gggaatgttg ctggcttcca ccatcatctt
     1021 atgtaactac accaacaaca ccacgctggt ggtcatgctg atggcgctgg ctttctttgg
     1081 taaaggattt ggtgcgctgg gctggccagt gatttctgac accgcgccga aagagattgt
     1141 tggcctctgc ggcggcgtct ttaacgtctt tggcaatgtt gcctccattg tcactccact
     1201 ggtgattggc tacctggtaa gtgaactgca ctccttcaat gcagcactgg tttttgtggg
     1261 atgttcagcg ctgatggcga tggtctgcta cctcttcgta gtcggcgaca ttaaacgtat
     1321 ggaattgcag aaataagcaa aggtataagc gatgaataac gatgttttcc cgaataaatt
     1381 caaagccgca ctggctgcga aacaggtaca aattggttgc tggtcagctc tctctaaccc
     1441 gattagcact gaagttcttg gtttggctgg gtttgactgg ctggtgctgg atggtgaaca
     1501 tgcgccaaac gatatctcca cgtttattcc gcagttaatg gccctgaaag gcagcgccag
     1561 cgcgccagta gtgcgagtgc cgaccaacga gccagtaatt attaagcgtc ttctggatat
     1621 cggtttctat aacttcctga ttccttttgt agaaacaaaa gaggaagcag agcaggcggt
     1681 ggcatcaact cgttacccac cggaaggcat tcgcggcgtc tccgtttctc accgcgccaa
     1741 tatgtttggc accgtggcgg attatttcgc tcagtcgaac aagaacatca ctattctggt
     1801 ccagatagaa agtcagcagg gcgtagataa cgtcgatgct attgccgcta ccgaaggtgt
     1861 agacggcatc ttcgtcggcc ccagcgatct ggctgcggca ttaggccatc tcggcaatgc
     1921 ctcgcacccg gatgtacaaa aagcaattca gcacattttt aaccgtgcca gcgcgcacgg
     1981 caaacccagc ggcatcctcg caccagtcga ggctgatgcg cgtcgttatc tggaatgggg
     2041 tgcgacgttt gtggctgtcg gcagcgatct cggcatcttc cgctctgcca ctcagaaact
     2101 ggctgatacc tttaaaaaat aaccaccacc gcaacaagag agatgattga tatgactatg
     2161 aaagttggtt ttattggcct ggggattatg ggtaaaccaa tgagtaaaaa ccttctgaaa
     2221 gcaggttact cgctggtggt tgctgaccgt aacccagaag ctattgctga cgtgattgct
     2281 gcaggtgcag aaacagcctc tacggctaaa gcgatcgctg aacagtgcga cgtcatcata
     2341 accatgctgc caaactcccc tcatgtgaaa gaggtggcgc tgggtgagaa tggcattatt
     2401 gaaggcgcga agccaggtac ggtattgatc gatatgagtt ctatcgcacc gctggcaagc
     2461 cgtgaaatca gcgaagcgct gaaagcgaaa ggcattgata tgctggacgc cccggtgagc
     2521 ggcggtgaac cgaaagccat cgacggtacg ctgtcagtga tggtgggcgg cgacaaggct
     2581 attttcgaca aatactatga tttgatgaaa gcgatggcgg gttccgtggt gcataccggg
     2641 gaaatcggtg caggtaacgt caccaaactg gcaaatcagg tcattgtggc gctgaatatt
     2701 gctgcgatgt cagaagcgtt aacgctggca accaaagcgg gcgttaaccc ggatctggtg
     2761 tatcaggcaa ttcgcggtgg gctggcgggc agtaccgtgc tggatgccaa agcgccgatg
     2821 gtgatggacc gcaacttcaa accgggcttc cgtattgatc tgcatattaa ggatctggcg
     2881 aatgcgctgg atacttctca cggcgtcggc gcgcaactgc cgctcacagc tgcggttatg
     2941 gagatgatgc aggcactgcg tgcagatggt ttaggaatgg cggatcatag cgccctggcg
     3001 tgctactacg aaaaactggc gaaagtcgaa gttactcgct aatgacgtcg tgcccggatg
     3061 gcgtattgca atccgggcct ggaatccagg ccgaataaga gaaacgccct ccggcgtcat
     3121 gtggtaacag gcataggtat gaaaatcgta atcgccccag actcttataa agaaagttta
     3181 tctgccagcg aggttgcgca ggcgatagaa aaaggatttc gggaaatttt tcctgatgca
     3241 cagtatgttt ctgttccggt tgccgacggt ggtgaaggaa cggtggaagc gatgattgca
     3301 gccacccagg gggctgaacg tcacgcctgg gttacagggc cgctgggcga gaaagtgaat
     3361 gccagttggg ggatctccgg cgatggcaaa accgcgttta tcgaaatggc ggcggccagt
     3421 gggctggaac tggtacctgc ggaaaaacgc gatccactcg tgaccacttc acgcggtact
     3481 ggtgagttaa tcctgcaggc gctggagagc ggtgcgacaa acattattat cggcattggc
     3541 ggcagcgcca ccaacgacgg cggcgcaggc atggtacagg cgctgggggc gaaattatgc
     3601 gatgccaacg gcaatgaaat tggttttggc ggcggtagtc ttaatactct gaatgatatt
     3661 gatatttccg gtctcgatcc gcgcttaaaa gattgtgtca tccgcgtcgc ttgtgatgtc
     3721 accaacccgt tagtcggcga cagtggtgct tcgcgcatct ttggcccaca aaagggagcc
     3781 agtgaggcga tgattgtcga gctggataat aacctctctc actatgccga tgtcattaaa
     3841 aaagcgctgc atgttgatgt gaaagatgtc cccggcgcag gagctgcggg cggtatgggc
     3901 gcggcgctga tggcgtttct tggtgcagaa ctgaaaagtg gtattgaaat cgtcacgacg
     3961 gcgctgaatc tggaggaaca tattcacgat tgtacgctgg tgatcactgg tgaagggcgt
     4021 attgatagcc agagtattca cggcaaggtg ccgattggtg tcgcaaacgt ggcgaagaag
     4081 taccataaac cggtgattgg cattgcgggt agcctgacca atgatgttgg cgttgtacat
     4141 cagcatggca ttgatgcggt cttcagcgta ctgaccagca ttggtacgtt agacgaagca
     4201 ctccgcggag cttatgacaa tatctgccgt gcttcacgta atatcgccgc gacactggcg
     4261 attggaatgc gcaacgcggg gtgacaaggg cgcgcaaacc ctctatactg cgcgccgaag
     4321 ctgaccagac agtcgctgct tcgtcgtcgt cctcttcggg ggagacgggc ggaggggagg
     4381 aaagtccggg ctccataggg cagggtgcca ggtaacgcct gggggggaaa cccacgacca
     4441 gtgcaacaga gagcaaaccg ccgatggccc gcgtaagcgg gatcaggtaa gggtgaaagg
     4501 gtgcggtaag agcgcaccgc gcggctggta acagtccgtg gcacggtaaa ctccacccgg
     4561 agcaaggcca aataggggtt cataaggtac ggcccgtact gaacccgggt aggctgcttg
     4621 agccagtgag cgattgctgg cctagatgaa tgactgtcca cgacagaacc cggcttatcg
     4681 gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt ttggtgccca
     4741 gggtaagatg aatat
//
LOCUS       DAZFRG010000314         4741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFRG010000314 DAZFRG010000000
VERSION     DAZFRG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.316"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..857)
                     /gene="kduD"
                     /locus_tag="ACHKUC_004575"
     CDS             complement(96..857)
                     /gene="kduD"
                     /locus_tag="ACHKUC_004575"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO6620688.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(887..1723)
                     /gene="kduI"
                     /locus_tag="ACHKUC_004576"
     CDS             complement(887..1723)
                     /gene="kduI"
                     /locus_tag="ACHKUC_004576"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO6620689.1"
                     /translation="MDVRQSIHSTHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2010..3191)
                     /gene="yqeF"
                     /locus_tag="ACHKUC_004577"
     CDS             complement(2010..3191)
                     /gene="yqeF"
                     /locus_tag="ACHKUC_004577"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO6620690.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEALARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3446..4675
                     /gene="yqeG"
                     /locus_tag="ACHKUC_004578"
     CDS             3446..4675
                     /gene="yqeG"
                     /locus_tag="ACHKUC_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6620691.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1242 a         1197 c         1047 g         1255 t
ORIGIN      
        1 tatggattat ttcataacca tgatatggat tatgatgatc tacagatata aaaaaccctg
       61 ccatgcggca gggtcataaa agtaagagga atgaattaac gcgccagcca accgccatcc
      121 acagcaatgg tataaccatt tacataatct gaagcgctgg aggcaagaaa cactaccggc
      181 cccatcaggt cactcggcag tccccaacgg ccagctggaa tgcggtcgag aatttccgcg
      241 ctacgttgtt catctgcccg cagttgttga gtattgttgg tcgccatgta acccggagct
      301 atcgcattaa cattaatatt gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc
      361 atcacaccgc tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc
      421 attgacgcga tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct
      481 gcctgagaca tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt
      541 tcgctgaatt cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca
      601 atatgaccaa actcagctac cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc
      661 agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg tgacctgctt gatggtttca
      721 gtcggttcaa cgatgttaat gccaacaatg tcacagcccg cttgcgccag ccccaacgcc
      781 atcccctggc ccagcccagt atcacaacca gtgacgaccg caactttacc ttcgagagaa
      841 aatgcactta aaatcataac aataccttat tcgtttatgc ccacaactag cgcaaatctt
      901 taacggccac atggtccata tcatcaaaaa cctggttttc accgaccatg ccccagataa
      961 aggtataagc tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct
     1021 gctcgttatg catcacaatg tgacgcgttt cttgcggttg ccccatcatg tggaaaacgc
     1081 aggcgtcatc atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac
     1141 acggcatggt gttccacaag ttacctggag ccagctccgt cagccccata ctcaattggc
     1201 aggtttccag cacatccggg acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt
     1261 tatcgcctaa cgtgactgga gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg
     1321 tatgcgcggg tgcgcaattg taataaaact tcgccggagt ggcggtatca atactggcaa
     1381 agacaacttc ttttgcacct ttaccaacat acagggcgtc gcggtgaccg atttcatagc
     1441 attgaccatc gacagtaatc gtaccggcac cgccaatatt gataacacct aactcgcgac
     1501 gttcaaggaa atagcttacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt
     1561 tagttaccgg cataatgccg ccaacaataa tacggtcaat gtggctgtaa accatggtgt
     1621 actcatcggc gacaaatacc ttttcaacca aaaattcatt gcgcagcccc tgggtatcca
     1681 gcgtttttgc gtgcgtactg tggatgctct gtcttacgtc cacatcaacc tccgataatc
     1741 agtgaagcgt gaaaatagaa aacaacaatg aaacactgtt tcgttttctc tatgagggga
     1801 tgatatgccc atgggatagg gttttcaatt aaaaataaaa caatgtttca ttttcaagtt
     1861 aaggattaaa aaaagtgcag tgtcgatcac gaatatgtca tgagaatgga aaaatcaggg
     1921 agccctgaaa aggtatgtcg actcaccagc catcattatt caacagatta atgacagcag
     1981 atatttatcg gtaaggagga aatgcagagc tactcgtcac gttcaatggt caatgccaca
     2041 ccctggcctc cgccgataca aagcgttgcc agtcctttgc gggcattacg tttcaccatt
     2101 tcatgaacaa gagaaaccag gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg
     2161 atcgcaccac cattgacatt gacccgacgc tcatcccatt caagcatctt gccaaccgaa
     2221 agcgcctgtg cagcaaacgc ttcattagcc tcgataagat cgacatcagc caactgccag
     2281 ccaacacgct ccaggcaacg gcgggtcgca tacaccggcg caattcccat caatgccgga
     2341 tccacaccaa cgctggcaaa tgcgcggatg cgggccagca cgggtaaatt caacgctcgt
     2401 gctttggctt cgctcatcat cattactgca gctgccccat cgtttatgga tgatgcatta
     2461 cccgctgtca cagaaccgag actatcaaat gaaggattta aacgggctaa ggcttctgcg
     2521 ctggtgtcag tgcgtggctg ttcatcggta tcaacaacca acgtctgacc gttactttgg
     2581 gttattaccg ggacgatctc atctttaaat cgtccggcgt caatcgccgc tcgcgctttt
     2641 tgttgcgagc taagtgcgta agcatcctgc aactgacggc tgatgccata ttcgcgagcc
     2701 agattttcgg cggtgacacc aatatgataa tcattgaagg catcccacaa cccatcatgc
     2761 acaagactat caaccaactg gctattgcca agctgtgcgc cggtgcggct atcagtcaga
     2821 acatgtggtg cgcggctcat gttttcctgg ccaccggcga tgacaatatc agcctcgcca
     2881 cactgtatcg cctgagtagc cagatgcagt gctttaagcc cggaaccaca aacgtcatta
     2941 atagtgattg cagaaacgct attaggaaga ccacctttaa tagccgattg tcttgccgga
     3001 ttctgccctg cccctgcagt caacacctga ccaagaatca cttcatccac cgcatatgca
     3061 ggaacgccgg tacgttctat taacgctttg acgaccaggc tgccaagttc cacggcggaa
     3121 tgacccgcta acgcaccacg aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca
     3181 acgtctttca tcacaacctc gcaccaatta acagtgcaat agtaaatgat tgttaattac
     3241 agttatctta attgtttaaa aaaagtgact attatcacaa aggaaatacg cctgagtagt
     3301 agtcagagac ataactggca cgtaaggttt gcaaccacta acccaccaat agagaggtag
     3361 ataaggcgtt aatctcccat acttaacctg gtttatggta aattgccctc cattttgttt
     3421 aatttgtaga tgatacgttc agataatgtc taatatttgg tcaaaagaag aaactctgtg
     3481 gagtttcgcg ctctacggca cagccgttgg tgcaggcacg ctcttccttc ctattcagtt
     3541 aggttcggcg ggggctgtgg tcctgtttat tactgctctg gtcgcctggc ctttaacata
     3601 ttggccacat aaagccttat gccagttcat cctctcatcg aaaacatcag caggtgaagg
     3661 gataacgggc gcggtaacac actactatgg caagaagatt ggtaatctga ttaccacgct
     3721 gtacttcatc gccttttttg tcgtcgtgtt gatatatgca gtggcaatta ccaactcact
     3781 tacggaacaa ctggcaaagc atatggttat tgatcttcgc atccgtatgt tggtgagtct
     3841 gggggttgta ttaatcctga atctcatttt tctgatggga cgccatgcaa ccattcgggt
     3901 aatgggattt ttggtattcc cattgattgc ctatttctta tttctttcta tttacctggt
     3961 cggtagttgg caacctgatc tattaacaac ccaggtagag ttcaatcaga atacccttca
     4021 ccagatatgg atatcgattc ccgtgatggt tttcgctttt agccatacgc ccattatttc
     4081 tacgtttgcc atagacagac gtgaaaaata tggcgaacat gctatggata aatgcaaaaa
     4141 aattatgaaa gtcgcttatc tcatcatctg cataagtgta ttgttctttg tctttagctg
     4201 cctgctttct attccacctt cgtatattga agcggctaaa gaagaagggg ttaccatttt
     4261 atcggcgctt tctatgctgc cgaacgcccc agcatggttg tcaatttccg ggattattgt
     4321 cgcagtagtt gcgatgtcga aatcattctt gggtacgtac tttggcgtta ttgaaggtgc
     4381 cacagaggtc gtcaaaacaa cattacagca ggttggtgta aagaaaagtc gtgcatttaa
     4441 ccgcgcacta tcaattatgt tggtatcgct gattaccttc attgtttgtt gcattaaccc
     4501 gaacgcgatt tcgatgattt acgcgatcag cggcccgctc attgccatga tacttttcat
     4561 catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa
     4621 tctgattacg ctgatcgtgg gtattctgtg cgtatcggta atgttcttta gctaatatcc
     4681 gttgtggatg gttcaaaatt gcgtcaattt tgaaccatcc cagttttcat aatgttcatt
     4741 t
//
LOCUS       DAZFRG010000315         4737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFRG010000315 DAZFRG010000000
VERSION     DAZFRG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.317"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..1277)
                     /gene="rsmC"
                     /locus_tag="ACHKUC_004579"
     CDS             complement(246..1277)
                     /gene="rsmC"
                     /locus_tag="ACHKUC_004579"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO6620692.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPYTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1380..1793
                     /gene="holD"
                     /locus_tag="ACHKUC_004580"
     CDS             1380..1793
                     /gene="holD"
                     /locus_tag="ACHKUC_004580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO6620693.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1762..2208
                     /gene="rimI"
                     /locus_tag="ACHKUC_004581"
     CDS             1762..2208
                     /gene="rimI"
                     /locus_tag="ACHKUC_004581"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6620694.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2223..2900
                     /gene="yjjG"
                     /locus_tag="ACHKUC_004582"
     CDS             2223..2900
                     /gene="yjjG"
                     /locus_tag="ACHKUC_004582"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO6620695.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            2991..4580
                     /gene="prfC"
                     /locus_tag="ACHKUC_004583"
     CDS             2991..4580
                     /gene="prfC"
                     /locus_tag="ACHKUC_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO6620696.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
BASE COUNT         1132 a         1271 c         1271 g         1063 t
ORIGIN      
        1 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcatac catcaattct
       61 taaaaagaat tgctaccacg gaggcgcatt ctagtggttt tcagcttttc gtcaatagtt
      121 aattatcgac agcggtgtaa ttgctggaaa aatgtccatc agcgaattgt ggataattcg
      181 tttgtcggtc agagaaggcg tgtacgccgc atccgacacg gtatgcggcg acatatatca
      241 gatactcaac ctttcttcgc ctggcgggtc ataatggcgc gatacacctt gaagcgcccg
      301 gtttgcgcaa tcacttcgtg gaaaccaaat gtctcatcca gcacgtccgg gtaaggcagg
      361 aaggcgttcg ctacaattcg caactcgccg ccgctgttca gatgacgcac cgcgccgcga
      421 atcagcgttt gcgcggcatc caggctggtt tgcatcccat cgtggaacgg cgggttggag
      481 attatcatat caaagcgacc tttcacctcg gaaaagacgt tactggcaaa gacttcacct
      541 tcaacaccgt tggtcgcaag tgttgcgcga ctggcttcta ccgctggtgc agaaacatca
      601 cacagagtga ggcggatctt cggtgaatgg cgcgcaaaag caacggaaag cactcccgcg
      661 ccgcagccga catccagcac tttacctttc gtgtatggag ttagcgtcga gagcagcaac
      721 tgactgccga catccagacc gtcgcggcta aacacgcccg gcagcgtttt gaccgtcaag
      781 ccatcgacgc tgtattcgcc ccagaattta ttggcatcaa ataccggctg cttttccaga
      841 cgaccaaaat agaggccgca gcgacgggcg ctgtctacct tattcaacgg cgcataatcg
      901 gccagcatct gctcggcgct gcgcacgccg ctgcggttct cgccaacgac aaaaatatcc
      961 gtgcccaccg gaagcagaga aagtaaattc atcaactgga actgggcttc cggtttgttc
     1021 ttcggccagt agtaaatcaa agtatcgcaa tcagcgacat catccgccgt ggcgaccagg
     1081 ctaaaacggg cgttatcccc catctggcgg cttaacacct gccagtggtg gaattgctgg
     1141 gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca ggtcatcctg taagtctccg
     1201 gcaaacagaa tacggctttg ttcgaaatca tcactgtgac gcagcaagac ttcacttgcc
     1261 ggggtaaatg cagacatgga atgctcctca attgatactg gcggcgatta tagccatatg
     1321 ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg acaacaggag cgattcgcta
     1381 tgacatcccg acgagactgg cagttacagc aactgggcat tacccagtgg tcgctgcgtc
     1441 gccctggcgc gttgcagggg gagattgcca ttgcgatccc gccacacgtc cgtctggtga
     1501 tggtggcaaa cgatcttccc gccctgaccg atcctctggt gagcgatgtt ctgcgtgcgt
     1561 taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga aaaaatcgcg atgctgccgc
     1621 aaggcagtcg ctgcaacagc tggcggttgg gtactgacga accgctatca ctggaaggcg
     1681 ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa cccaacggca cgcgccgcgt
     1741 tatggcaaca aatttgcaca tatgaacacg atttcttccc tcgaaacgac tgatttacca
     1801 gcggcttacc acattgaaca acgcgcccac gcctttccgt ggagtgaaaa aacctttgcc
     1861 agcaaccagg gcgagcgtta tctcaacttt cagttaacgc aaaacggcaa aatggcggcg
     1921 tttgcgatta cgcaagtggt gctggatgaa gctacattgt tcaatattgc ggtcgatcct
     1981 gactatcagc gtcagggact gggaagggcg ctgctggaac atctgatcga cgaactggaa
     2041 aaacgcggcg tggcgacgct atggctggaa gtccgtgcct caaatgctgc cgccattgcc
     2101 ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc gcaattacta ccccaccacg
     2161 gacggtcgcg aagacgccat catcatggcg ttaccaatca gtatgtaaga caaggtggaa
     2221 taatgaagtg ggactggatt ttctttgatg ccgatgaaac gctgtttacc tttgactcat
     2281 tcaccggcct gcagcggatg tttcttgatt acagcgtcac ctttaccgct gaagattttc
     2341 aggactatca ggccgttaac aagccactgt gggtggatta tcaaaacggc gcgatcactt
     2401 cattacagct tcagcacggg cggtttgaga gctgggccga acggctgaac gtcgaaccgg
     2461 gtaaactcaa cgaggccttt attaatgcga tggcggaaat ctgtacgccg ttgccggggg
     2521 cggtttccct gcttaacgcc attcgtggca acgccaaaat cggcatcatc accaacggct
     2581 ttagcgcctt gcaacaggta cgtctggaac gcacgggcct gcgtgattac ttcgatttgc
     2641 tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa gaaaattttc gattatgcgc
     2701 tggaacaagc gggcaatcct gaccgttcac gcgtgctgat ggttggtgac actgccgagt
     2761 ccgatattct cggtggcatc aacgccgggc ttgcgacctg ctggctgaat gcgcacaatc
     2821 gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc ttcgttgcac gaactggagc
     2881 agctcctgtg taaacactga ttgcctcccc cccgttgatg ggtaaaatag ccgcaatttt
     2941 tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga agaaagaatt atgacgttgt
     3001 ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc cattatttct cacccggacg
     3061 ccggtaagac taccatcacc gagaaggtgc tgctgttcgg acaggccatt cagaccgccg
     3121 gtacagtaaa aggccgtggc tctaaccagc acgctaagtc ggactggatg gagatggaaa
     3181 agcagcgtgg gatctccatc actacgtctg tgatgcagtt tccgtatcac gactgcctgg
     3241 ttaacttgct cgataccccg gggcacgaag acttctcgga agatacctat cgtaccctga
     3301 cggcggtgga ctgctgcctg atggttatcg acgccgcaaa aggtgtagaa gatcgtaccc
     3361 gtaagctgat ggaagttacc cgtctgcgcg atacgccgat cctcaccttt atgaacaaac
     3421 ttgaccgtga tatccgcgac ccgatggaac tgctcgatga agttgagaac gagctgaaaa
     3481 tcggctgcgc accgatcacc tggccgattg gctgcggcaa gctgtttaaa ggcgtttacc
     3541 acctttataa agatgaaacc tatctctatc agagcggtaa aggccacacc attcaggaag
     3601 tccgcattgt taaagggctg aataacccgg atctcgacgc tgcggttggt gaagatctgg
     3661 cacagcagct gcgtgacgaa ctggagctgg tgaaaggcgc gtctaatgag ttcgacaaag
     3721 agctgttcct tgcgggcgaa atcaccccgg tattctttgg tactgcgctg ggtaacttcg
     3781 gcgtcgatca tatgctggat ggcctggtgg agtgggcgcc tgcgccgatg ccgcgtcaga
     3841 ccgatacccg taccgtagaa gcgagcgaag ataaatttac cggcttcgta tttaaaattc
     3901 aggccaacat ggacccgaaa catcgcgacc gcgtggcgtt tatgcgcgtg gtatccggta
     3961 aatatgaaaa aggcatgaag ctgcgccagg tgcgcaccgc gaaagatgtg gtgatctccg
     4021 acgcgctgac ctttatggca ggcgaccgtt cgcacgttga agaagcgtat ccgggcgata
     4081 tcctcggcct gcacaatcac ggcaccattc agatcggcga tacctttacc cagggtgaga
     4141 tgatgaagtt caccggtatt ccgaacttcg cgccagaact gttccgtcgt atccgcctga
     4201 aagatccgct gaagcaaaaa cagctgctca aagggctggt acagctttcc gaagaggggg
     4261 cggtgcaggt gttccgtccg atctccaaca acgatctgat cgttggcgcg gttggtgtgc
     4321 tgcagtttga tgtggtggta gcgcgcctga agagcgaata caacgttgaa gcagtgtatg
     4381 agtcagtcaa cgttgccacc gcccgctggg tagaatgtgc ggacgcgaag aaattcgaag
     4441 agttcaagcg taagaacgaa agccaactgg cgcttgatgg cggcgataac ctcgcttaca
     4501 tcgctaccag catggtcaat ctgcgcctgg cgcaggaacg ttatccggac gttcagttcc
     4561 accagacccg cgagcattaa ttctagcctc cagggcgcgg tagccgctgc gccctgtcaa
     4621 tttcccttcc ttattagccg cttacggaat gttcttaaaa cattcacttt tgcttatgtt
     4681 ttagctgata tcccgagcgg tttcaaaatt gtgatctata tttaacaaag taatgac
//
LOCUS       DAZFRG010000316         4710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFRG010000316 DAZFRG010000000
VERSION     DAZFRG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.318"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..295
                     /locus_tag="ACHKUC_004584"
                     /pseudo
     CDS             197..295
                     /locus_tag="ACHKUC_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
     gene            376..804
                     /locus_tag="ACHKUC_004585"
     CDS             376..804
                     /locus_tag="ACHKUC_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO6620697.1"
                     /translation="MKRSGGTRYMYLIALITVAAALTACTPKTSLEQHTRHYVYASDD
                     GFDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEKFLQSLR
                     STTTFAGRKYTDSDMPSPEKMKLLADTISAVYLDGYEGRQ"
     gene            1351..1635
                     /locus_tag="ACHKUC_004586"
     CDS             1351..1635
                     /locus_tag="ACHKUC_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO6620698.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            1635..1910
                     /locus_tag="ACHKUC_004587"
     CDS             1635..1910
                     /locus_tag="ACHKUC_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6620699.1"
                     /translation="MELKWTSKALSNLARLYDFLVLASKPAAARMVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(2176..2670)
                     /locus_tag="ACHKUC_004588"
                     /pseudo
     CDS             complement(2176..2670)
                     /locus_tag="ACHKUC_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2947..3822
                     /locus_tag="ACHKUC_004589"
     CDS             2947..3822
                     /locus_tag="ACHKUC_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFO6620700.1"
                     /translation="MRKFILISMIALASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPAVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATSTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            3830..4606
                     /locus_tag="ACHKUC_004590"
     CDS             3830..4606
                     /locus_tag="ACHKUC_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFO6620701.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQTLVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT         1279 a         1149 c         1083 g         1199 t
ORIGIN      
        1 tggatatccc gggttcacag gaggcagcaa cgagctgctg tttaaattcg ggaggataat
       61 tagggcagcc ttttcgcctg ccgggagtca catttttctg catatctgac actttggttc
      121 ccactactta tttggtggac accactttgt ctaatttgtc agattctgac cagacggttc
      181 aggctgtacg cttacatcag aacgggaaca gttacagaat gagatagccc agctcaggca
      241 ggcactggaa caggaaaaga aacggggatt ctggtccaga ctcttcggtc actgaactcg
      301 gtcagaggct gatgataaga tagccatcag ataataactc accgaaaata aatactttaa
      361 ggtaaggaga ctcgcatgaa acgttctggc ggcacacgct atatgtatct tatcgccctg
      421 ataactgtgg cagcagccct cacagcctgt accccaaaaa ccagcctgga acaacatacc
      481 cggcattatg tttatgcttc agatgatggg tttgatccta acttctacac caagaaagct
      541 gacaccacac ggatgatgat tccgttcttt cagcagttcc gggagatggg ggtgaaagac
      601 aaagcagccg gagtatcagc agaaacggca cagcaacgca taaaagaatt ccactcagaa
      661 aaatttcttc agtctctcag aagcacaacc acctttgcgg gcagaaaata cacggacagt
      721 gatatgccat caccggaaaa aatgaagctg ctggctgaca cgatttctgc ggtttatctc
      781 gatggctacg agggaagaca gtaagtggat ttaccataat cccttaaatt gcacgtacca
      841 tctgaaacgc tctcagacgc aactctaggc tgaaccagaa aaatgcgtca gaatcgtttt
      901 cagcgcgctg tataaccatg ctcgaaagcg atgagcgatc ggtttcgtgc gttctcctgt
      961 tccgaccctg ccgtttgccg gatacctgtc cgcctttccc ccttcgggaa gcgtggcgct
     1021 ttctcatagg ggtcagtttg gatatagaga attattgtac ggtaagcctg ttttttgaac
     1081 aggcttacct atcgcgatag ggggtacccc cacgctatat gccatttctc gtggattttg
     1141 tctaatcgga taacctaaat gcgatacgga ttacacgata atttaaaagc gatagccact
     1201 ggcactaaat caatccctta cggggtgact tggttataaa taatgcgcta aacgtacaat
     1261 aatttccaat attcaaactg accccattat ttgccttgtg ccacctgaat gtaatcacta
     1321 tgattaaata tgattaacaa ctaatcggat atacaaatga aaaacaatac cgcacaggca
     1381 acaaaagtaa ttaccgcgca tgtgccatta cctatggctg ataaagtcga ccagatggcc
     1441 gccagactgg aacgctcccg gggctgggtt atcaaacagg cactttctgc atggcttgcc
     1501 caggaggagg agcgtaatcg cctgacgctg gaagccctgg acgatgtgac atccggacag
     1561 attatcgacc atcaggctgt acaggcctgg gcggacagcc tcagtactga caatccgtta
     1621 ccggtgccac gctgatggaa ctgaagtgga ccagtaaggc gctttctaat ttggcgcggt
     1681 tatatgattt tctggtgctg gccagtaaac ctgctgccgc cagaatggta cagtccctga
     1741 cacaggcacc ggtcattctg ttaactcatc cacgtatggg agaacagttg tttcaatttg
     1801 aacccaggga ggtcagacgg atttttgctg gcgagtacga aatccgttac gaacttaatg
     1861 gccagactat ttatgtattg cgcctgtggc acacacgaga aaacaggtag attgcgtgtg
     1921 gggacaactt aacatagcct gcagatatta attctggaac attattatca ggccagatat
     1981 tttgcattcc attaaataat cttgacgtat aggaaattga aaaactgagc gatacccctg
     2041 tccgcgcctt atgccacgtg agcttttctg cttttcccac cactccggtg ttttctctct
     2101 atgtatggtc ggtttttata ccgtaatggg ccgtttttta tacttttcct ggattgcagg
     2161 cgcacctctc ctgcatcagc cagggatata cctcagcagg cttatatccc gcgcgttttt
     2221 cttcagccct gatacattca gccccgcaat ggcccggtgg tataccatcc ggcagccaca
     2281 cagcacgcat tcgaacgggt cacgactcag gaactgcttc accatttgtg catagcacac
     2341 tttcgccact gcttccggtt tatccatccc cagagcacgg tacacctgcg gcagcttctc
     2401 tccacacacc cggttggcaa ggaacccgaa gtacctcacc atcttaaaaa acttctccgg
     2461 gatgtgctgt ttcagcctcg cgaccagctc acgctgtgtc agcgtttccg tcgccgtttc
     2521 tcccgttttg tggtccaggt aacggaagct caggctcgcc cctccgttgt aatgtcccag
     2581 ccgggaggct gctatcggcg gcttcttcag ataacgaccc aggtagcgcg ccgtattcct
     2641 cccgccggcc gttttcttcg acatgtacat caggcgcgga actcatcggg gcgcgggaat
     2701 gaaatcccat tcagactccg gatagattca agcaagccca cttatcgtca aaatcggtgt
     2761 tgcaaaaacg ggagtgacca tagatccgtt ttccaagcgg atcccataat atcggctgat
     2821 aaacatcact cgctagtgca ttctttttgt tggtaatgat aatcatttac attcattctt
     2881 tgatgtttta tcataattaa tgttttgtca ataaatatat ggaaacatat tgtttcagat
     2941 gggaatatga gaaagttcat ccttatttct atgatagcgt tagctagtgt tagtctggga
     3001 gcctgcagac agaatgtcac cataaagcag gatgcgccag ggcagaaagc tttcctgact
     3061 gacgggcaga tttacgattt gcacagtggc aaaattattt ctagcagcga gcttttggcg
     3121 gatcttgcta ccgctcgaca tcttattatt ggtgaaaaac acgataatgc cgagcatcat
     3181 cagattgagc tttggcttat tcaaaatctg ctcatccagc gaccacaggg gagtgtgttg
     3241 cttgagatgt tgacgtcaga gcaacagccc cgagttaacc aagttaaatg ttggctgaaa
     3301 gataatcctg tggttcgaga tagccgtgtt caggaactac ttaactggca aaaagggtgg
     3361 tcctgggaga tgtatggcga catcgtcatg cagctattac gtgggcctta cccgctgctc
     3421 aatgccaaca ttggccggga gcagatgttg gcactgtaca aaaaaaacga atttcctaaa
     3481 ggaaaaaaat ccactgcacc tgcggtacaa gaagctctgc gtgaaaccat catagctatg
     3541 catgaaggca accttgaaag ccagcaactg acgtcaatgc tctctgttca gcagcagcgg
     3601 gatcgctaca tggcccgcca gcttctgagt gccccggtac cgtcgttgtt gattgcaggt
     3661 ggttatcacg cgtcaaaaag catgggcgta ccgttacaca tggaagatct tgccaccagc
     3721 acccatccag tggtgttgat gcttgcagaa aaaggaatga acatcacagt agatcatgct
     3781 gactacgtgt ggtttgtagc gccagataca acaaaaaggt aaaccatgga tgatgaatat
     3841 tctccacttc ccacagtcgg taaaatggag cagctggttt atctgttctc tactgcttca
     3901 tggactgatt ttccttgcgt ttatctggcg tttttccgaa gttcagcccg ctatgtctcc
     3961 tgcaccggcg atcatgctgc aatgggcaga aattatagag gctccttctt ctccactttc
     4021 gcttccggtg ggaatcgctc aacaggagtc cgcagtgaca gaagaaaagc aacaaactga
     4081 ggacagacaa caacgtcctg tcactgaaga cagtgatgca accattgaaa taactaggaa
     4141 gaaaaagtca tccgatggag aaaagaaaaa gacacgtcca cctcgtaaga taaaggctca
     4201 aaccagcgac agcaacccta cagccgtttc cagtaatgct gcacctcaga ctctggtcga
     4261 atcgtcccgt attgctgcgc cttttaacag tgactcaaca aagcgagata attctgaggc
     4321 atcttgggaa agcagagtaa aaggccacct taaccgttat aaacgttacc ctggcgatgc
     4381 cagaaaacgt gcacgaaccg gcacagccgt cgtgaccttt accgtaaaca cggaaggcac
     4441 tattgtcagt agtttcttgg aaatctcttc aggaaccctt tctcttgacc gcgaagctat
     4501 tgctgtttta gaacgcgctc aaccacttcc caaaccaccg cctgaaatat tggagggagg
     4561 attatttaaa gtaaaaatgc cgattacctt taagttgaag gagtaaaggg ataattggcc
     4621 gttcaagttc atttaactga aatttaattt tcatttttat gctgaacatt ttatcaaact
     4681 tttccactgt cgaaaaaatc aaaaagacag
//
LOCUS       DAZFRG010000317         4688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFRG010000317 DAZFRG010000000
VERSION     DAZFRG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.319"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(26..965)
                     /locus_tag="ACHKUC_004591"
                     /pseudo
     CDS             complement(26..965)
                     /locus_tag="ACHKUC_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(967..1821)
                     /gene="istB"
                     /locus_tag="ACHKUC_004592"
     CDS             complement(967..1821)
                     /gene="istB"
                     /locus_tag="ACHKUC_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFO6620702.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGLLTKNTTPISDDEMVKADSISNESILAGMKMA
                     NNGQTSWR"
     gene            complement(1821..2993)
                     /gene="istA"
                     /locus_tag="ACHKUC_004593"
     CDS             complement(1821..2993)
                     /gene="istA"
                     /locus_tag="ACHKUC_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFO6620703.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVNNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            complement(3097..3372)
                     /locus_tag="ACHKUC_004594"
                     /pseudo
     CDS             complement(3097..3372)
                     /locus_tag="ACHKUC_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3374..4280
                     /locus_tag="ACHKUC_004595"
                     /pseudo
     CDS             3374..4280
                     /locus_tag="ACHKUC_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3655..3771
                     /locus_tag="ACHKUC_004595"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            4357..4493
                     /locus_tag="ACHKUC_004596"
                     /pseudo
     CDS             4357..4493
                     /locus_tag="ACHKUC_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856299.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1108 a         1208 c         1161 g         1211 t
ORIGIN      
        1 agaattctgt gtcacgttaa aaattctaca gcgtcatcac attattcagc cgcccttcaa
       61 aataaatggc taactgcgac aatgtcaaat tccagctttg gatgggcatt gtccatttct
      121 cctgtgcgtt catcagtccc agataaagta gcttcaacag gctgttttca ttcgggaaag
      181 cacctttagg ttttggtcag tttcctgaac tggcggtgga ctgattctat cgcgttggtt
      241 gtgtagatca ccttgcggat attcgccgga tagcggaagt acgctgacag attttccctc
      301 ttgcggcgcc aggactggag caccaccgga tattgctgcc cccgtttcgc ttccagctca
      361 tccagcgccg tttccgctgc ttcttttgac accgcacgat acactggctt caggtcggcc
      421 atgaacgctt tatggtgttt tgacgccaca tatttaatcg agttacgtat ctggtgaatg
      481 acgcacaact gtacctctgt ctgcggataa atgctgttta tcgcttcagg gaagccggtc
      541 agcccatcca cgcaggcaat cagaatgtct tcaacgccac ggttttgcag atcacttaat
      601 actgacagcc agaagttagc gccttcgctt tccgacaaat acaggcccag gacttctttt
      661 ttgccttcca gattcagcgc cagtacggta taaactgcct tgctctgata acacccatct
      721 tcgcggattt tatagtgaat ggcgtccagc cagacgaagg gataaacctt ttccagcggg
      781 cgctgctgcc actgttttaa ctcaggaatg actttatcgg taaccgcact gatggtagcc
      841 gtggaaacac tgaaggcata taaatcttcg atctcccggc tgatatcctg gtagctcatc
      901 cccagcgcga acaggcgaat gatcttgcgc tcgagctcat cagacaacgt ggtctggtgc
      961 ttcttgtcaa cgccacgatg tttgaccgtt atttgccatt ttcatgcccg ctaagatact
     1021 ttcgttactg atgctgtccg ctttcaccat ttcatcatca ctgattggcg ttgtgttttt
     1081 ggtcagcaat ccagctttac gtttctcttt taaccggtaa ctctctcctt tgatattcag
     1141 cgtggttgag tgatgtagca accgatccag tatcgctgtt gccagcacgt gatctccgaa
     1201 catttctccc cagtctgcga accctttgtt tgacgtcagt atgatgctcg ctttttcata
     1261 tcgacggttc agtagccgga agaacaggct ggcttcctct ctgttcatcg gcagatagcc
     1321 tatttcatcc aggatcaaca cccgggcata actcagttgc tgcagctgac gctccagccg
     1381 gttttcctgt ttcgctttca tcagtgtcgc gatcagtctg tccagtggca taaacagtac
     1441 ccgatgtccc gcatccaccg ctttcacgcc aagagctatg gccagatgag tttttccgac
     1501 accaggaggt cccagcagga tcacgttttc gctgcgctcc acgaacgcca gaccagccag
     1561 ttcccggaca accttacggt cgatgcccgg ctggaaggta aagtcgaact gctccagcgt
     1621 tttgacccac ggcagacgag cctgcttcag cctggactcc ataccgcgct gatgcctgcc
     1681 gttccattcc tgctgtagcg ccatgcacag gaactcccgg tagttcagct cttttttagc
     1741 tgcctgttcc agcaggcttt caacgtgata actcagatgc tccattttca ggcgactcag
     1801 caggacttcc agctcatgca tcacaacagc tcctcatagg cacttagtgg tcgatgttcc
     1861 acctgactga cctgctgcca gagcggggcg tgatgctccg gcactgtctg ccagccagac
     1921 gatgccgaac agaggcgatg tgaggccacc agtttctcat tactgtagat ccgcaactcg
     1981 tcatccagcg atattcgtat cgataccggc tgaccacaca gcgcttcggg aacgctgtaa
     2041 cgattaccac caacctcgat atagctgtcc caggacacat ggcggatgtc gaagtaactg
     2101 gtatcgaagt ccgtatccgg taacggctgc agatgttcct gctccagcgc gaagcgctgt
     2161 tccggcgttt ctttgaactg gcgaagttcc cgtttgtcag ccacatcggc tatccattgc
     2221 tccagttgtt gattaacatg agtgaagctg tcgaacctgc ggtaccgaac gaagaagttc
     2281 tccttgaggt atttcaccat ccgctcaacc ttaccttttg ttctggccct gcgtggacgg
     2341 catgcccgtg gcaggaagtt atagtggtcg gccagcaaca ggaatccgga gttgaacacg
     2401 actttcccgt tgttattctt cagcaccgca gccttctggt tattaaccag caccgttttc
     2461 acacaaccac cgaagtagcg gaaggcgcga accagtgatt cgtaggtatg ctcagcatcc
     2521 tgttttggtg cggcgaagac atggaagcgg cgggagaacc ccagcgtatt aaccgcaaag
     2581 ttaactttgc accgttgccc ggcaacctcc acctcaactt cgccccagtc gtgctggagc
     2641 tggtatccag gctgagtttc gaagcgaact gttctttttg acggacgcat tttacgtttg
     2701 ggctggatgt agtaacgcag catggaacgt ccgcccgtat aacccattgc cttaatctcc
     2761 gcaaagataa cctcactatt ccagacattc tctgccaggc gcatgtcgat gtaatccata
     2821 aacggtttca gcttaaccat tttgtggcgg gtctttctgg ctggcggttc agggtatttg
     2881 aggtagcgtc tgaccgtccg ttcagagcaa ccaatctgag tcgcaatatc gacaatgtac
     2941 gcgccctgct ggcgcatttg ctttatcatg taaaagtcct ctctgctcag catgttgatg
     3001 tcctttctgg tgtgagaacc ttaaggaaac aacatgttgg gtggagcgga caatccaaat
     3061 ggtgaattac cgtcttatat cactggcgct gacattcttc accagttggg gttcaaaata
     3121 accattacga tcacgtggag ttgccagctc aaagctgcca gtcggggctt taatggtctt
     3181 ttttccggaa ccatttttac gattgacttc aacatcctga gccagatggg agtcgagttc
     3241 agcggccaga gcggtttctg ttaactgttt gattaatggc gttaggatac catccttgcc
     3301 ggttaatgct tggccggatt gaaccgcccc gggaatcctg gagactaaat tccctgagaa
     3361 agaggtaaac aggatgacta aaaatacacg tttttccccc gaagtccgtc aacgggcagt
     3421 tcgtatggtt ctggaaagtc aggacgaata tgactcacaa tgggcggcaa tttgttccat
     3481 tgctccaaag actggctgta cgccagagac tctgcgtgtc tgggttcgcc agcatgagcg
     3541 ggataccggg ggcggtgatg gtgggttcac caccgctgaa cgtcagcgtc tgaaagagct
     3601 ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc cttcgccagg cttccgctta
     3661 ttttgcgaag gcggagttcg accgcctctg gaaaaaataa tgccactgct ggataagctg
     3721 cgtgagcagt acggggtcgg accggtatgc agtgaactgc atattgcccc gtcaacgtat
     3781 taccattgtc agcagcagcg gcatcatcct gataaacgca gtgcccgtgc gcagcgcgat
     3841 gactggctga agagagagat acagcgcgta tacgatgaaa atcatcaggt gtacggtgtg
     3901 cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg tggccagatg tacagtggcg
     3961 cgcctcatgg cggttatggc tctcagtatg tgtcactggc ctatatggag cgattaaaag
     4021 aagcaaaact gctggcatca acagggagta caggcgactc gtatgacaac gcgatggcgg
     4081 agagcatcaa tggtctttac aaagcggagg taatacaccg taagagctgg aaaaaccgtg
     4141 cagaagtgga actggccaca ctcacgtggg tggactggta taacaatcga cgattgctgg
     4201 gaaggctggg ccatactcct ccggcagaag cagaaaaagc ttattatgct tccatcggaa
     4261 acaatgatct ggcagcctga gttcacaaat aaaacactct ccaggaaacc cggggcggtt
     4321 cacttagccg cgtgtccatt attgctggat aagatcacaa cgatacaccg ggttaaacag
     4381 gtggtcactg cttcactgaa gctgcgtgat tatgatggtc aggtagcaga agctatgccc
     4441 atgataccaa aatgacgaaa gctgaaatgt ctgaaagcgt gtgtattatc tgaaaatatg
     4501 accggctccg gggtacgtaa ccgattaaac aaagccaatc gatattacaa aacgagaggg
     4561 actatatatt tcgttttcag ataagactcc ttgagagatt atcttttatg taaaatctca
     4621 aatatcacct gatgtaatgt caatgataat attaagtgcg ctctgaacaa catctttctt
     4681 ggtgtgat
//
LOCUS       DAZFRG010000318         4687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFRG010000318 DAZFRG010000000
VERSION     DAZFRG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.320"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..923
                     /locus_tag="ACHKUC_004597"
     CDS             93..923
                     /locus_tag="ACHKUC_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6620704.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            955..1890
                     /locus_tag="ACHKUC_004598"
     CDS             955..1890
                     /locus_tag="ACHKUC_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6620705.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1983..2225
                     /gene="yhdT"
                     /locus_tag="ACHKUC_004599"
     CDS             1983..2225
                     /gene="yhdT"
                     /locus_tag="ACHKUC_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO6620706.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVSAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2215..3666
                     /gene="panF"
                     /locus_tag="ACHKUC_004600"
     CDS             2215..3666
                     /gene="panF"
                     /locus_tag="ACHKUC_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO6620707.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3678..4559
                     /gene="prmA"
                     /locus_tag="ACHKUC_004601"
     CDS             3678..4559
                     /gene="prmA"
                     /locus_tag="ACHKUC_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO6620708.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT         1057 a         1175 c         1240 g         1215 t
ORIGIN      
        1 acccatgatt ttcttttcga attgtcgata tgattgaagg ataactttca aagaaaaata
       61 caaggggcta tagtgttacg cattaaaggc caatgaagag tgttaacgtg ccgagaaaat
      121 cgtctaacct actgaaacgc agattaaaaa ttgcagaaat tgttgccgcc cagggtgaaa
      181 tcaaagttga tgacttatct gcgcaacttg gtgtctctgg cgtgacgatc cgcggcgatc
      241 tcagttatct ggagcaacag ggatatttaa agcgatcatt tggcggtgct attgcgacac
      301 cactctcctc tgaaccagaa gcgatacaac cgatcgcgcc attgtctctg gcacaacaaa
      361 tggaaatagc acgacattgc gcacgattaa ttcgcgatcg tgacacgcta tttcttggac
      421 atgggacaat ctgccgtaag atcattccat tgctcagcga agtgaaaaaa ctgcgtctga
      481 tcaccaacga tcccgaacat gcgctgctgg caaatcagtt tattgatggc gaaataatac
      541 tggctggcag tgaaatgttg cgccccgaca ccgcactggc cggaaaacag cttgaacagg
      601 cacttcagca tttcaccatt acacatagca ttctggaaat cagccatctg gatgctgatg
      661 gtactttaaa tatcaacgtt cccagactcg ccgctgcctg gcagctctgt ttcgatcatg
      721 cccagaataa gacggttatc gttgccgccg atcctgcgcc atcaacggct gctatcgggc
      781 atttgcatca ggcagaaaat gtgatcgtca gccgcagcat taacgaacaa tatcagcaac
      841 aattgcagtc ggctgatttc gttatccttt ataccagtaa tgagtgcttt acctggtcca
      901 atcgcgaaag gcttcaggaa tgagaggggt tttgtgacaa caactatctg cgctatgggc
      961 gaattgctgg ccgagttttt gtcccgcaac ccacatcaaa aattcactca gcctggggag
     1021 tttatcgggc catttcccag cggtgcgcca gcaatttttg ctgctcaggt ggcaaaactg
     1081 tcccatcggg ccatcttctt tggatgtgtt ggtaatgatg attttgcccg actcattata
     1141 gagcgtctcc gtcatgaagg tgtcattacc gatgggatcc atgttatgaa caatgccgtc
     1201 acaggtacgg cgttcgtgag ttatcaaaat ccccagcagc gggatttcgt ctttaatatc
     1261 cctaacagcg cctgcggttt gtttactgcc gagcacattg ataaggatct gcttaaacag
     1321 tgtaaccatc tgcatattgt gggctcatcg ttgttctcat ttcgcatgat cgatgtcatg
     1381 cgtaaagcaa taacgacgat caaatcggct ggcggcaccg tttctttcga tcccaatatt
     1441 cgcaaagaga tgctgagcat tcctgaaatg gcgcaggctc tcgattattt gattgaatat
     1501 acggatattt ttatccccag cgaaagcgaa ctccctttct tcgcgcgtca caaaaatctg
     1561 tcagaggaac agattgttag cgatcttctc cacggcggcg taaaacatgt ggcgataaaa
     1621 cgcgcccagc gtggggccag ctattacaag cttaaaaacg gtacattaca cgcccagcat
     1681 gttgcaggtc acgatatcga aattatcgat ccaacgggtg caggcgactg ctttggcgca
     1741 acgtttatca ctcttttctt atccggtttc ccggcacaca aggcgctgca atatgcaaat
     1801 gccagcggcg cgctcgccgt aatgcggcaa ggtccgatgg aagggatatc ctcactggca
     1861 gacattgaag actttttgca gcagcactga acggcaattt tctgtcagat cattttccct
     1921 gcctgaagac ttgccgtaga attgcccgtt tctcgtatcc ccccatgagg aggacccctt
     1981 ccgtggacac tcgttttgtt caggcccata aagaggcgcg ctgggcgctg gggctgaccc
     2041 ttttgtatct ggcggtatgg ttagtatccg cttacttacc tggcgttgcc cccggtttta
     2101 ccggctttcc gcgctggttt gagatggcct gcatcctgac gccgctgctg tttattggac
     2161 tgtgctgggc gatggtgaaa tttatctatc gcgatatccc actggaggat gacgatgcag
     2221 cttgaagtaa ttctaccgct ggtcgcctat ctggtggtgg tgttcggtat ctctgtttat
     2281 gcgatgcgta aacggagcac tggcaccttc cttaatgagt atttcctcgg cagccgctct
     2341 atgggcggta ttgtgctggc gatgacgctc accgcgacct atatcagtgc cagttcgttt
     2401 atcggcgggc ctggagctgc ttataaatac ggcctgggct gggtattgct agcgatgatt
     2461 cagcttcctg cagtctggct ttcgctcggt attctcggca agaagttcgc aattcttgcg
     2521 cgccgctata atgcggtgac gctgaacgat atgctgtttg cccgctacca gagtcgtctt
     2581 ctggtatggc tggcgagttt gagtttgctg gttgcgttcg ttggtgcgat gaccgtgcag
     2641 tttatcggcg gtgcgcgcct gctggaaacc gcggcgggta ttccttatga aaccgggctg
     2701 ctgattttcg gtatcagcat tgcgttgtat accgcctttg gcggctttcg cgccagcgtg
     2761 ctgaacgaca ccatgcaagg gctggtgatg ctgattggca ccgttgtgct gcttattggc
     2821 gtggtacatg ccgcaggtgg tttaagtaat gcggtagaca cgctgcaaac catcgatccg
     2881 caactggtta cgccacaagg cgctgacgat attctgtcgc ctgcctttat gacgtcgttc
     2941 tgggtactgg tgtgctttgg cgttataggc ctgccgcata ctgcggtgcg ctgtatctct
     3001 tataaagaca gcaaagccgt acatcggggg atcattatcg gtacgattgt agtcgcaatc
     3061 ctgatgttcg gtatgcatct ggccggagcg ttaggtcggg cggtgatccc cgatctcacc
     3121 gtaccggatc tggtgatccc aacgttaatg gtaaaagtgc tgccaccgtt tgctgccggg
     3181 atcttcctgg ctgcgcctat ggctgcgatc atgtcgacaa ttaacgccca actgctgcaa
     3241 agttccgcta cgatcattaa agatctctat ctgaatatcc gtccggatca aatgcaaaac
     3301 gagacgcgtc tgaagcggat gtcggcggtg atcacgttgg ttctcggtgc gttgctgctg
     3361 cttgccgcct ggaagccgcc agaaatgatc atctggctga atttgctggc cttcggtggg
     3421 ctggaagccg ttttcctgtg gccgctggtg ctgggtcttt actgggaacg cgccaacgcc
     3481 aaaggcgcgc taagtgcgat gatcgttggc ggcgtgctgt atgccgtact cgcgacgctg
     3541 aatattcagt acctgggctt ccaccctatc gtgccctcgt tactactaag tttgctggct
     3601 ttcctggtcg gaaaccgttt cggtacatcc gtcccgcaag ctaccgtttt gactactgat
     3661 aaataaagag ttttgccatg ccttggatcc aactgaaact gaacactacc ggcgcgaacg
     3721 cggaagatct tagcgatgcg ctgatggaag cgggtgccgt ttctatcact tttcaggata
     3781 cccacgatac gccagtattt gagccgctgc cgggcgaaac gcgcctgtgg ggcgacaccg
     3841 atgtgattgg tctgttcgac gctgaaaccg atatgaacga cgtggtggcg attctggaaa
     3901 accatccgct gctcggcgcg ggcttcgcgc ataaaatcga acaactggaa gataaagact
     3961 gggagcgcga atggatggat aatttccacc cgatgcgctt tggtgaacga ctgtggatct
     4021 gcccaagctg gcgtgatgtg ccggacgaaa acgccgtcaa cgtgatgtta gacccagggc
     4081 tggcgtttgg tacgggtacc catccaacta cctctctgtg cctgcaatgg ctcgacagcc
     4141 tcgatttaac cggtaaaaca gtcatcgact ttggctgcgg ttccggcatt ctggctattg
     4201 cggcgctgaa actgggtgca gcaaaagcca ttggtattga tatcgatccg caggcgatcc
     4261 aggccagccg cgataacgcc gaacgtaatg gcgtttctga tcgtctggaa ctctacttac
     4321 cgaaagatca gccagaagaa atgaaagccg acgtggtggt cgctaacatc cttgcaggcc
     4381 cattacgtga actggcaccg ttaatcagcg tcctgccagt ttcaggcggt ttgctgggcc
     4441 tttccggtat tctggcaagc caggcagaga gcgtttgcga agcttatgcc gacagcttcg
     4501 ctctggaccc ggtcgtggaa aaagaagagt ggtgccgcat taccggtcgt aagaattaac
     4561 cttcgcatcg ccgtatggcg acgcagagcg aagtgcgagc gatgtcacaa aaggcacgtt
     4621 aatttgccga ttatttacgc aaatttgcgt gccaaaattt tcattcataa agaaaaattg
     4681 agaactt
//
LOCUS       DAZFRG010000319         4655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFRG010000319 DAZFRG010000000
VERSION     DAZFRG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.321"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..1695
                     /gene="recN"
                     /locus_tag="ACHKUC_004602"
     CDS             34..1695
                     /gene="recN"
                     /locus_tag="ACHKUC_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO6620709.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPLAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            1844..2185
                     /gene="bamE"
                     /locus_tag="ACHKUC_004603"
     CDS             1844..2185
                     /gene="bamE"
                     /locus_tag="ACHKUC_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6620710.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(2247..2537)
                     /locus_tag="ACHKUC_004604"
     CDS             complement(2247..2537)
                     /locus_tag="ACHKUC_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO6620711.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(2527..2964)
                     /gene="ratA"
                     /locus_tag="ACHKUC_004605"
     CDS             complement(2527..2964)
                     /gene="ratA"
                     /locus_tag="ACHKUC_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO6620712.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            3135..3617
                     /gene="smpB"
                     /locus_tag="ACHKUC_004606"
     CDS             3135..3617
                     /gene="smpB"
                     /locus_tag="ACHKUC_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO6620713.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3831..4193
                     /gene="ssrA"
                     /locus_tag="ACHKUC_004607"
     tmRNA           3831..4193
                     /gene="ssrA"
                     /locus_tag="ACHKUC_004607"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1291 a         1184 c         1100 g         1080 t
ORIGIN      
        1 aacagtaatg gtttttcata caggaaaaca actatgttgg cacaactgac catcagcaac
       61 tttgctatcg ttcgtgagct tgagattgat tttcatagcg gcatgaccgt aataactggc
      121 gagaccggcg cgggtaaatc tattgcaata gatgccctcg gcctttgtct cggtggtcgc
      181 gctgaagccg acatggtgcg taccggcgct gctcgtgccg acctgtgcgc ccgtttttct
      241 ctgaaagata cgccagcggc cctgcgttgg ctggaagaaa accagcttga agacgggcat
      301 gaatgtttgc ttcgtcgcgt aatcagcagc gatggtcgct cccgtggttt catcaacggt
      361 acagctgttc ctctgtcaca actgcgcgaa ctgggtcagt tgctgattca gatccatggt
      421 cagcacgctc atcagttact caccaaaccc gagcaccaaa aattcctgct tgatggctat
      481 gccaatgaaa cctctcagct ccaggaaatg accgcacgtt atcatttgtg gcatcaaagc
      541 tgccgtgacc tcgcgcatca tcaacagtta agtcaggaac gcgccgctcg tgcggagctg
      601 ctgcaatacc aattaaaaga acttaacgaa tttaatccgc agcctggaga gtttgagcaa
      661 atcgacgaag agtacaaacg tctggcgaac agcggtcaat tgctgaccac cagccagaat
      721 gcattggcat taatggctga cggtgaagac gcaaacctgc aaagtcaact ttacacggct
      781 aaacaactgg tgagcgaatt gattggcatg gacagcaaac tgtccggcgt acttgatatg
      841 ctggaagagg ctaccatcca gattgctgaa gccagtgatg aactgcgcca ctactgcgat
      901 cgtctggatc tcgatcccaa ccgactgttt gaacttgaac agcgcatctc aaaacagatt
      961 tcgctggcac gtaaacatca cgtcagccct gaggcattgc cacagtatta ccagtcgcta
     1021 ctggaagaac agcagcaact ggacgatcag gccgactcac aagaaacgct cgcgctggcg
     1081 gtaacgaaac atcatcagca ggcactggaa acggcgcgcg cgttacacca gcaacgccag
     1141 cattatgcaa atgagttggc acagctgatc accgatagta tgcatgcact ctctatgcca
     1201 catgggcagt ttacgatcga tgttaaattt gacgagcatc acctgggcgc tgacggcgct
     1261 gatcgtattg agtttcgggt aaccaccaac ccaggtcagc caatgcagcc tcttgccaaa
     1321 gtcgcatccg gtggtgagtt gtcccgcatc gcattggcaa tccaggtcat taccgcgcgt
     1381 aaaatggaaa ccccggcact gatttttgat gaagtggatg tagggattag cggcccaaca
     1441 gcggcagttg tcggcaaact gctacgtcaa cttggcgaat caactcaggt gatgtgtgtt
     1501 acccacctgc cacaagtcgc gggatgtggt catcaacact attttgtcag caaagaaact
     1561 gatggtgcga tgacagaaac gcatatgcaa tccctggata aaaaagcgcg gttacaagag
     1621 ttggcgcgcc tgcttggtgg cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa
     1681 ctgcttgcgg cgtaaacttt tttcctgctt cacggtcaga gtaaacagca aaacgccgta
     1741 agaccggaaa gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg catattacag
     1801 atgagccacg tactgctcgg gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct
     1861 gactgctgca gcagcagtac tattgatgtt gaccgcaggc tgttccactc tggagcgagt
     1921 ggtttaccgt cctgacatca accaggggaa ctatctgacc gctaacgacg tatccaaaat
     1981 acgtgttggc atgacgcaac aacaagttgc gtacgcattg ggtacaccgc tgatgtccga
     2041 tccatttggt acaaatacct ggttctatgt cttccgccag caacctggtc atgaaggtgt
     2101 aacgcagcaa acgctgacgc tgacctttaa cagtagcggt gtgttgacca acattgataa
     2161 caaacctgcg ctgagtggta actaataata aagttgctct caaagacgtt aaaaaaggtg
     2221 ctcaatgagc accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg
     2281 cgaagctctt tcggatcggc aatgagagga cgataaatct ccacccgatc gccatcatgc
     2341 acaatatcgc tgagttttgc cggacggctg taaatgccga ctttgttttt agttaaatcg
     2401 atatcggtac gcaattccag caaaccactg gcgcgaatag cttcttcaac cgtcgcgccc
     2461 tcctgcaacg tcacgcgctg caggtactgc ttctcaggta gcgcataagc cacctcaacg
     2521 gcaattttac ctggcactgt agacctcttt tgcacgaaca gtaaaagcct gcaccatatt
     2581 agctgccagc tctttaaaca cacgaccaaa ggcgagttca atcaacttat tggtaaactc
     2641 aaagtcgaga tgaaactcaa tacggcacgc gtcctggctc aacggcgtaa acttccaacc
     2701 gccgatcaat ttcttaaacg gtccatcaac cagactcatg agaatacttt gattactggt
     2761 caactggttg cgggttgtaa acgttttgct gatcccagcc ttagacacat ctaccgcagc
     2821 agtcatctgc ccaggagtgg actccagaat ccgacttccg gtacaacccg gcaaaaactg
     2881 aggataagac tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggcaccag
     2941 tgcggtccgg ctaatctgag gcataacaat ttccatcaac aaaaatccaa caaataatat
     3001 catttatcct gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta
     3061 agatagagcc ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat
     3121 gattcacgac gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc
     3181 ttaacaagcg cgcccgtcac gaatacttta tcgaagaaga gttcgaagcg ggacttgccc
     3241 tgcaaggctg ggaggttaaa tccctgcgcg caggaaaagc caatatcagc gacagctatg
     3301 tccttctgcg cgacggagag gcatttctgt ttggcgctaa catcacgcca atggctgtgg
     3361 cctccacgca tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg
     3421 aactggactc attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc
     3481 tgtactggaa aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aagaaacagc
     3541 acgataaacg ttcagatatc aaagaacgcg aatggcaggt ggataaagcg cgtatcatga
     3601 aaaacgccca ccgttaaacc tatataagcg atactaatca aatcctcacc gcgcctccct
     3661 atccggtggc gcgaatgaac atcttatcga ctgtcaaatc tgacacagat gtagccatcc
     3721 cattgcttaa tcgaaaaaaa tcatactatg caggcgctaa ttccaggatg ataacgcctt
     3781 ttgggctagt catgctgctc ataaatctgg tatacttacc gttacacatt ggggctgatt
     3841 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     3901 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     3961 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     4021 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     4081 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     4141 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga
     4201 ttggacggtg taaggactac accaacaaaa acaggaagtt ataagtctca gcaggacacc
     4261 gaccagacgg tgaggagaca aaaaaggata cgcaacggag tcgcgactcc ctatgacatt
     4321 aaagcccgct gatgcgggct tttttattgg tcctgacccg ccctgaatag aattcatcca
     4381 attacagtcc agacatatac agtatccctc agctcagtat cagcttctcg acaaatggtc
     4441 actgttattt acccgttgcc agaaagagaa taatttgctc ccatagccag ccttcccgga
     4501 atctgtatat tcattaatct tgttatcgat tccatcaagt aacataggtt ggatgcgatt
     4561 gatcttactc acattagtcg acacggaact cagtaagttg aactctggaa aagcagttta
     4621 aatagcattt aaacaaatgc caccagttcg aaaaa
//
LOCUS       DAZFRG010000320         4618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFRG010000320 DAZFRG010000000
VERSION     DAZFRG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.322"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            223..2655
                     /gene="hsdR"
                     /locus_tag="ACHKUC_004608"
     CDS             223..2655
                     /gene="hsdR"
                     /locus_tag="ACHKUC_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO6620714.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWRKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDEEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLNDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2722..4191
                     /locus_tag="ACHKUC_004609"
     CDS             2722..4191
                     /locus_tag="ACHKUC_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6620715.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4191..>4618
                     /locus_tag="ACHKUC_004610"
     CDS             4191..>4618
                     /locus_tag="ACHKUC_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6620716.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRLGDFTNIIRGITFPGNEKSQFQAPGKIACLRTA"
BASE COUNT         1338 a         1128 c         1131 g         1021 t
ORIGIN      
        1 gtagataaat tagcagaatg acatctaatg ccttagtaca aaaatatttt ttcactcctt
       61 gcaagaaccg aacattcccc atatgaatcc ttatccagct attgatgaaa ctcaagaata
      121 cctttaaaac agtgaattaa cgctgataat actctttgaa cagatgaatt catctatttt
      181 tctgtgtaga atttgcgcat cgcatttaga tgagaacacc ccatggcgga actgaaccta
      241 agtaacctga cggaagcaga catcattacc aaatgcgtta tgccagccat tctcaatgca
      301 ggctgggaca acacaacgca aatcagacag gaggtcaaac tccgggacgg taaagtcatc
      361 gtacgtggta aagttgcggc acgcagaacg gtaaaatctg ctgacatcgt gctgtatcac
      421 aaaccaggca ttcccttagc cgtgattgaa gcaaaagcca acaaacatga aattggcaaa
      481 gggatgcaac agggcattga atatgcgcgc ctgctggacg ttccttttgt ttttgccacc
      541 aacggggatg gctttatctt ccgcgatgcc accgcagccg aaggtgaatg ccttgaaaag
      601 caaataacgc tggatgactt cccctccccc gctgaactct ggcgaaaatt ctgcctctgg
      661 aaaggataca cacaagctca gcttccggtg attactcagg actattacga cgatggcagc
      721 ggtaaatcgc cacgttatta ccagcttcag gcaatcaaca aaaccattga agccgtctcc
      781 aacgggcaaa accgcgttct gctggtcatg gcgaccggaa cagggaaaac ctataccgca
      841 ttccagatca tctggcgcct gtggaaatca aaaaataaaa aacgcatttt gttccttgcc
      901 gatcgcaaca ttctggtcga ccaaaccaaa aataatgatt tccagccatt tggtacggca
      961 atgaccaaag tcagcggacg caccattgat cccgcttatg aaattcacct cgcgctctat
     1021 caggctataa ctggcccgga ggaagaccaa aaagcgttta aacaagtcgc accagatttc
     1081 ttcgatctga tcgtgatcga cgaatgccat cgcggcagcg catccgaaga cagcgcctgg
     1141 cgagagatcc ttgattattt cagttccgcc acccaaattg gcttaaccgc cacgccaaaa
     1201 gagacgcatg aagtctccag cacggattac ttcggcgatc cggtttacgt ctactcacta
     1261 aaagaaggga tcgaagacgg cttcctcgcc ccttataaag ttgtccgtgt tgatattgat
     1321 gttgatctgc aaggctggcg cccaaccaaa gggcaaactg acttaaacgg cgaagtgatc
     1381 gacgatcgta tctataacca gaaagatttc gatcgcacga tggtaatcga cgaacgcaca
     1441 gaactggttg ccagaaccat taccgactac ctcaagcgca ccaacccgat ggataaaacc
     1501 atcgtcttct gtaacgacat cgatcatgca gaacgtatgc gccgcgccct ggtgaatctc
     1561 aacccggagc aggtgaaaaa gaacgacaaa tacgtcatga aaatcaccgg cgatgatgaa
     1621 attggcaaag ctcagttgga taacttcatc aacccaaaaa aaccgtaccc ggttatcgcg
     1681 accacttcag agctgatgac caccggtgtg gatgctaaaa cctgcaaact ggtagtactg
     1741 gaccagaaca tccagtcgat gaccaaattc aagcaaatta tcggtcgtgg tacacgcatc
     1801 gacgagcgtt acggcaaact ctggtttacc atcctcgact ttaaaaaagc caccgaactg
     1861 tttgccgatg agcgtttcga tggcattccc gaaaaagtta tggataccac accagaggat
     1921 atcgccgatc cagaatctga ttttgaagag aaactcgaag aaatcagcga acacgacgaa
     1981 gagcaggtga caggcgttga tgaaccgcct gcgccgccat accaggttac cgataccgat
     2041 gatgttggcc cacttccgga agaagacgag aagaaaatcc gcaagtttca cgtcaatggc
     2101 gtggcggtgg gtgttattgc gcagcgtgtt cagtattacg atgccgacgg taaactggtt
     2161 accgaatctt ttaaagatta cacccgcaaa acactgctca aagaatatgc ctcgctgaat
     2221 gactttaccc gcaagtggca ggacgccgat cgcaaagaag cgatcattca cgagctggag
     2281 caacagggga tcatctggga agtactggca gaagaagtcg gtaaagatct cgacccgttc
     2341 gacatgcttt gccacgtagt gtatggtcag ccgccgttaa cccgcaaaga gcgcgccgag
     2401 aacgtgcgca agcggaacta cttcacaaaa tactctgaag cagcgcaagc cgtgctcgat
     2461 aatctgctgg ataaatacgc cgatgcgggc gtacaggaga tcgaaagtat tcaggtgctg
     2521 aaacttaaac cattcgacag catgggcacc ttaccggaga ttattaaaac cggatttggc
     2581 gaccgtaacg ggtataatca ggcgctcagc gagctggaaa acgaaatcta ccaattaccg
     2641 ccccgctctg cttaacaact ctcttttgcg ccttctgcaa ccatcgtgtt gcagaaggcc
     2701 ttgtttttca tggaatacaa tatgtctatc agttcagtaa tcaaatcatt acaagatatt
     2761 atgcgcaaag atgccggtgt ggacggcgat gcgcagcgtc tcggtcagct ctcctggcta
     2821 ctgtttttga aaatcttcga tgcccaggaa gaggcgctgg aactggagca ggataactat
     2881 caatatccga tcccacagcg ttatttatgg cgcagttggg ccgcaaacgc tcagggcatt
     2941 accggtgatt ccctgctgga attcgttaat gatgatctgt tcccggcgtt aaaaaacctc
     3001 actgcgccta tcgataaaaa cccacgcggc tacgtggtaa agcaggcgtt cagcgatgcc
     3061 tataactaca tgaaaaacgg tacgctactg cgtcaggtga tcaacaagct gaacgaaatt
     3121 gactttacca gcgccagcga acggcatctg tttggtgata tttacgaaca gatccttaaa
     3181 gatctgcaat ctgcgggcaa tgcgggcgaa ttctatactc cacgcgccgt cactcgcttt
     3241 atggtggatc gcgttgatcc gaaactcggc gaatccatta tggacccggc ctgcggtacg
     3301 ggcggttttc ttgcctgcgc atttgatcat gtaaagaaca aatacgtgaa gagcgtcgcc
     3361 gatcatcaga cgctgcaaca acagatccac ggtgttgaga aaaaacagct tccgcacctg
     3421 ctggcgacca ccaatatgct gctacacggc attgaagtgc cagtgcaaat tcgtcacgac
     3481 aacactctga acaaaccgct ttcctcctgg gatgagcaac tggatgtcat tgttaccaac
     3541 ccgccgtttg gtggcacgga agaagacggt attgagaaga actttccagc agagatgcaa
     3601 acccgcgaaa cggcggattt gttcctgcaa ctgattgtgg aagtactggc gaaaaacggt
     3661 cgtgcggcgg tggtattgcc ggatggcacg ctatttggcg aaggcgttaa aaccaaaatc
     3721 aaaaagctgc ttaccgaaga gtgcaatctg cataccatcg tgcgtttacc gaatggtgtg
     3781 tttaacccct ataccggcat taaaaccaac ctgttgttct ttaccaaagg tcagccaacc
     3841 aaagagattt ggttctatga gcatccgtat ccggcgggcg tgaaaaatta cagtaaaacc
     3901 aagccgatga agtttgaaga gtttcaggcg gagatcgact ggtggggtaa cgaggccgat
     3961 ggttttgcca gccgcgtcga gaatgagcag gcgtggaaag tcagcattga tgatgtcatt
     4021 gcgcgcaact tcaatctgga tattaaaaac ccacatcagg cggaaaccgt tagccatgat
     4081 ccggacgaac tgttagcgca gtatgcaaaa cagcaggcgg agatccagac gctgcgtaat
     4141 caactgcgcg atattcttgg cgctgcgctg tctggcaagg aggttaacta atgagtgtgg
     4201 aaaagctgat cgttgatcat atggaaacct ggacctcggc gttgcaaacc cgttccaccg
     4261 ccgggcgcgg cagttccgga aaaattgatt tatatggcat taagaaatta cgtgagctga
     4321 ttctggaact ggctgtgcgc ggtaaactgg tgccgcagga tccgaatgat gaaccggcgt
     4381 cggagctgct gaagcgtatt gcggcggaaa aagcagagct ggtgaaacag gggaaaatta
     4441 aaaagcaaaa accactgccg gaaattagcg aggaagagaa gccatttgaa ttgccggagg
     4501 ggtgggagtg ggtgcgattg ggtgacttta ccaatatcat tcgtggcata acttttccag
     4561 gaaatgagaa gtctcaattt caagccccag gtaaaatagc ttgtttacga acagcgaa
//
LOCUS       DAZFRG010000321         4607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFRG010000321 DAZFRG010000000
VERSION     DAZFRG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.323"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..387
                     /locus_tag="ACHKUC_004611"
     CDS             <1..387
                     /locus_tag="ACHKUC_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFO6620717.1"
                     /translation="EKNKLKSFSLAKIKWLDIKKEKFAKSEKIQSLISESCDPWVSDK
                     TFDVVLFIHSNIAHYFLRRDLLPYQQLLHKHDNGITLSCKAAHKNQIIPLILYWLPNV
                     EIIEPVWLKEAVLTMLGKYLTAGNVS"
     gene            436..1515
                     /locus_tag="ACHKUC_004612"
     CDS             436..1515
                     /locus_tag="ACHKUC_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFO6620718.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDILEKAKERYE
                     EHKGKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIHNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACNVERAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITTQQ
                     DISVSDDVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKDELDDVLASSVVKFDYFSSQS"
     gene            1562..3133
                     /locus_tag="ACHKUC_004613"
     CDS             1562..3133
                     /locus_tag="ACHKUC_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFO6620719.1"
                     /translation="MNVEKFDWTEELHQTMVKSLATSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGDYDSHHYHSHENYIATNRKGKRAHQAGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRVCQKRVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTEK
                     MIVRLIREMWNNLVQAFKILVFNPEGLSPGQLAKAVTKLVAAGVAVAGGVMINEAMAQ
                     LLIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFRRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAALNHFSLHLDSVNSEIERGLLLREEVSRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            3130..3396
                     /locus_tag="ACHKUC_004614"
     CDS             3130..3396
                     /locus_tag="ACHKUC_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6620720.1"
                     /translation="MKLHPPFPCNQCGACCRHVNRAGETQCLDRGDGICRHYQTDSHL
                     CAIYDKRPQICRVEDQYLLNYQSQYSWQEFIALNQAACLILNKL"
     gene            3536..3733
                     /locus_tag="ACHKUC_004615"
     CDS             3536..3733
                     /locus_tag="ACHKUC_004615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620721.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRSLLSRKKTS"
     gene            3799..4440
                     /locus_tag="ACHKUC_004616"
                     /pseudo
     CDS             3799..4440
                     /locus_tag="ACHKUC_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601293.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
BASE COUNT         1303 a          876 c         1131 g         1297 t
ORIGIN      
        1 gaaaagaaca agttaaagtc gttttcatta gctaaaataa aatggctgga tataaaaaaa
       61 gaaaagtttg caaagagtga gaaaatacag tcactgataa gtgagtcatg tgacccgtgg
      121 gtgagcgaca aaacatttga tgtagtgttg ttcattcaca gtaatatcgc ccattatttt
      181 ttgcgacggg atttacttcc ttaccagcaa ttgctacaca aacatgacaa tggcattacg
      241 ctgtcatgca aggctgcaca taaaaatcaa attatcccgt tgattttata ctggctaccg
      301 aatgttgaaa ttattgaacc agtatggctg aaagaagccg tgttaacaat gttaggaaaa
      361 taccttactg ctgggaatgt ttcctgaaat atttcagttg actgatgata aagcaatctg
      421 tacaggagga aaagaatggc gttacctttt attattttgg gtgtggctgc cggcgctgcg
      481 gcattcggcg gaaaaaaagc ttacgatgga tatcaaaaaa agtcagaagc agaggatatc
      541 ctcgaaaaag caaaagagcg ttacgaggag cataaaggaa aatttgagca ggctgaagct
      601 cagacatcat caaaattaac aacgctcgga aataaagagc ttgagattgg taaagatttc
      661 agtgagttcg acaggattgc tgctgagctc ctggcgcgtc tggaaaaaga gggcaataaa
      721 gacctgaaac tgagtgtccc gcaacataac atcaataaga ttcataacct ggccatttca
      781 gcaacagaat atctcagtac ggtcgttgct gccggagttt ccggggctgc tgcgggtttt
      841 gctgtgtata gcggagtgat ggcatttgct gcggcttcca caggtacacc cattgcagcg
      901 ctttctgggg ccgcggctta taacgcgacg atggcggcaa ttggtggggg ctcactggct
      961 gcagggggca tgggaatggc tggtggcgca atggtattag gcactgcagt ggctgcaccg
     1021 ttgattgcca ttgcgggctg ggcttacgac agacatgcga cgaaagctct tgaaaatgcc
     1081 catcagtgtg cctgcaatgt tgaacgggca gttaagaaaa tgaatctggc ccgggagcat
     1141 tttgcaaaag ttaatcaata cgttgatgaa attctcgctg cactgaacag aatgcatgct
     1201 gtatttatta aacactattt tgagccactt aaatccatgc attctgttat tacaacacaa
     1261 caggatatca gtgtatcaga tgatgtcatt gtgcttattg accatggata ttcactggcc
     1321 gctattatga cggatattat tacaacgcca ctgtttaaac cgcggaaaca ggaaaatggt
     1381 gaggcagtga taaaagataa cgttattgaa atggaaacgg atgataatgg aatgaacgtt
     1441 attaataagg atgagctgga tgatgtattg gccagttcgg ttgtgaaatt tgattatttt
     1501 tcatcccaga gttaatgatt taccgggcgt taaagcccgg ctttacaaga agtgaaataa
     1561 catgaatgtg gaaaaatttg actggacaga agagttacat cagaccatgg tgaagagcct
     1621 ggctacatca ttcgggctgg attttctgtt gttaaatgat cgtaaggggg gtgatgttga
     1681 cactatccat aatgtgcgta atggcattta tgccacggag gctgagcgac agcggtatga
     1741 aggtcgtggc gactatgatt ctcatcatta ccacagccat gaaaactaca tcgcgacgaa
     1801 ccggaaagga aagcgtgcac atcaggcagg gactcttact gatgcatata caggcgaagt
     1861 gtttgcttca gacgataaaa aaaatctgga tcatattatt tccgctaaag aaattcatga
     1921 tgatccgggg aggattcttg ctgaacggga tggcgcagaa ctggcaaatg atgcttcaaa
     1981 tctggcattc actcatgaat cgctcaataa atcaaagaaa gcagattcaa tggatgcgtt
     2041 tatttgcaca ctgcagaaaa acagagaaga tacgttaagg cagatagcag agcttgaatc
     2101 tcaggcaacg ctgacagaaa aggaaaagaa acgtctgata agtctgagaa aaaaagctga
     2161 tgcagatact gagcgtatgg aaagggctga taaatatgcc cgggaaaaat atgaggccac
     2221 gattaatcag agctattacc tgagtagtaa gtttgctact gctgtcgcca gtgcttcact
     2281 taattctggt tttcgtatgg ggacgcgaca gatgctgggg cttattcttg ccgagatctg
     2341 gttcgaactg agggaacgaa tcccgcatat tatcaggcag caacggaact cattcagcgc
     2401 cggcaaatgc ctgtccgaaa taacgtcagc attacgtgtc tgccagaaac gagtacagga
     2461 aaaattcagt tcttttttaa ttgcctttaa agatggagtc atcggaggga tactgtcagg
     2521 tattaccaca acgctgttaa atatattcct gacaacggaa aaaatgattg tccggcttat
     2581 cagggaaatg tggaacaatc tcgttcaggc ctttaaaatc ttggtgttta acccggaggg
     2641 attatcgccg gggcagttag ccaaagcggt cactaaactg gtagcagcag gtgtggctgt
     2701 tgccggaggg gtgatgataa atgaggcgat ggcgcagctc cttatttttc catttggttc
     2761 agagcttgct gccttttgtg gtgcgctgac gacaggtatt ctcgggctgg tgatgaatta
     2821 ttatctcgaa tacagcgagg ttatgcagcg ggtgtgggca tttctggaca aatttaagga
     2881 taagttccgg cggtcactgg aatactttca ggcagtcaat gctgaacttg atcgctatct
     2941 tgtagaactt actgcgctgg aattttctgt tgatgctgcc gcgttaaatc acttttcgct
     3001 tcatctggat agcgtaaaca gtgagataga gcgcggactt ctgctgagag aggaagtcag
     3061 ccgacgaggg attgcgttgc cgtttgagtc aggaaatgtg gcttctgttc gcgactggtt
     3121 aaataagcga tgaaattaca ccctccattt ccctgtaacc agtgtggggc ctgttgtcgt
     3181 catgtcaaca gggctggtga aacgcaatgt ctggatcgtg gtgacggtat ttgccgtcac
     3241 tatcagactg attctcatct gtgtgcaatt tatgataaac gcccacagat ttgccgggtt
     3301 gaagaccagt atttactgaa ttatcagtct cagtacagct ggcaggagtt tattgcatta
     3361 aaccaggccg cctgccttat tctgaataaa ctgtaatacc attaatggct gcgtcagtac
     3421 agaaactgag agaacataaa gcagtaaaat gtcataccgt tcagcagcca gcgttgcctt
     3481 ccccggttgc tgttattgta aggcattttc gttaagaatg cagaaggaga ggagcgtgag
     3541 attgctgtta aaagggctcg tcggaatggc gctggcttgg cttgttattg gtttcgggat
     3601 ggagttattt tttctcgccg atcgggttca tccggcatta agagtaattg ttgtcctggg
     3661 gatgttattc ctgctgataa tgattgcggg cggtatgtgc cgttctcttc tgtcacgaaa
     3721 gaaaacatca tgacgatcct gtttacgccg ctacatttgt ggcggcggat gatgcccgtt
     3781 ttatgattat tcagaaaaat gtatttacct gccacgccat attcagcgac cagcgtcgga
     3841 cagaaatagc gctttttctc aattattatg aaaattaatc ttccgttcat aaactggtca
     3901 cagattaaca atgataagcg ccttgtgtta tcagtggttg tctttaccct ttctaatctc
     3961 cttgtatcct ccggcgcgca tctgatctac cgggtgttat tctttatcct ggcacttctt
     4021 attattaagg cgatgaaatc gcttacgctg cgttttattc ttgccttttc gttcacatta
     4081 ctgacagcgg cagatatcag tatcagtttg tattcatggt gtacattcgg tacaacattt
     4141 aatgatggtt ttgcaatcag tatccttcag acagagcctg atgaagtaat aagaatgttc
     4201 agaatgtatg tcgtttatgt tattgcgttt attatcctct ttttattgtt cttctgttct
     4261 gcaataaaga aatcgtcttc atttttttcg aaaaagaaca ctgtaaccac gtttttattg
     4321 ttaattacag tagcgttatg ttcttccttt cagtttgcgt tgaagaaaca gtatcaaata
     4381 aatgaagttg atccttacat agtcgcttcc cggtttgcca catatacagc tttcctccac
     4441 tgcgtataat gattatctga ttaatgcatg gatgggggta acaaagccgg aacaaccgaa
     4501 aacgctggag gaggtgatta cccgctggca gggaaaatca gatgtttttg atgcaaacca
     4561 taacgtgttt gactataacg tattgcgaaa gaactttaat gaacccc
//
LOCUS       DAZFRG010000322         4586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFRG010000322 DAZFRG010000000
VERSION     DAZFRG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.324"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..699)
                     /locus_tag="ACHKUC_004617"
     CDS             complement(<1..699)
                     /locus_tag="ACHKUC_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO6620722.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRC"
     gene            complement(715..3195)
                     /locus_tag="ACHKUC_004618"
     CDS             complement(715..3195)
                     /locus_tag="ACHKUC_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6620723.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVSNI
                     RLEDSQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDSFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLQEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGHQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(3211..3885)
                     /locus_tag="ACHKUC_004619"
     CDS             complement(3211..3885)
                     /locus_tag="ACHKUC_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6620724.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKVTFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3966..4508)
                     /locus_tag="ACHKUC_004620"
     CDS             complement(3966..4508)
                     /locus_tag="ACHKUC_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6620725.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT         1291 a         1103 c          846 g         1346 t
ORIGIN      
        1 acaacgcaga ataatatccc cttttttact atttattgtt gttttgttac ctgtggctgg
       61 taattgtgaa acgggaatac tctctaacgt aattaataaa tcgtccggtt tgcaggttgt
      121 ggttatcggg ttgtatgaca atgtcagatt aaaggaatag acacctgcat tttgtgccaa
      181 tgcatttctg tagctattta cacatgcatc ccatgactta ccacctaata aacacagtag
      241 aaattgtact gcggcactcc cgcgtgtcga tgatgaagca ctggataatg tcgcaactcc
      301 attaatcatc actgaaccac cgccccccgc agaaacattc acttttgcat tactggccgc
      361 aggtgtcacc gtcaccgaat aaccactggt gaaactggtc tgattatttg acaacgtaat
      421 gttgttttta ttggtccatt caaaatgtaa atttaattta atggtatcgt tataaagtcc
      481 aacaataata tccgacgggc tcataggtgt gatagtattt gtactcacgc atgacgaatc
      541 attaaaatta tgctcatatg tcgccatttg tgtttgagta agtgcaaaat ccccctgaac
      601 aaccaaattg tgctgaacag ataactcact ataacatgcc gcatagctca caggcataac
      661 catcatcatt actataaata gcattatgcg aatttccata gaaccacctg actattactg
      721 gcaaatataa tttctacttt catcaatctc tttaccgaag gtgattgtgc atgaaagccc
      781 ttgttgctta tcaattgcca cattaaccga tggcggtact tcattggtgc gaataaataa
      841 ctggcttccc tggccgacaa caccaatatt atgaccatgg atatcattga cttcataacc
      901 aaacgttaat ggttgcccat ccgctcttaa cgcttttata aaccatggct tgcgctgatc
      961 ggtatcaaaa ttaaccagta caaccgcgcc gcgataaggg gcggcaattt tacggttgcc
     1021 acgtaattct gcttcgctat cgctttgtga cacatccaac atcaggtaat tttcccgata
     1081 aggtgtcatt ccgtcgtata ccaccactcc attacggttt gttgtgcgat atttttgccc
     1141 attgacataa gcatctttaa ttcctggcgc attcatcaca gcaaacgttt cagaaagacg
     1201 gtttgccaga ttaacgccac ccgaccaggc gacaatgccc cctgaaacac tggctccagc
     1261 ctgtcgataa gcactcgact gactataact gccattcact gtcgcaaccg gcgcgttcca
     1321 ggttaaattc gccccagctg tcgtttcatt tccctgatac tgataactca ggttgacccc
     1381 atagttaaac tgatcccggc ttccaacggt tcctgataat cccgtattat ttgaggcaac
     1441 cccctgatca tcaaacgtcg ttgagttaga catatatatt tgccgacgag gcgtcgtaac
     1501 gtcatcaccc caatcaaagg gaatcgatat aaaaatatta aaacgtttct cttcatgatg
     1561 attctcgtca taagcatggc ttgccgcgag ggtatagctt atccttcgca ggttgttgga
     1621 ataactcaac tgataatcct tactgctgcc gctacgcccc cagtaatctc gccataacgt
     1681 acttaaggac acagaacccc agccttctgg caatgactga ctcatattgg cagagaagct
     1741 atttttacgg ccaaaatcgt tctggtaata atcggcaatg tcatagatat cgttttcatc
     1801 acggcgataa ttatctttat tgtttgccca tacgtgatca ttaaatgtcc gataatcacg
     1861 cgacgaataa cgccaggccg ccagaccaaa acgtgtcgac gtttggctca caaatttgtt
     1921 gtaggcaatt tgataacttt gcccgtcaaa cacatcgccg ttgtcttgtt tactatgtga
     1981 cttcgtggca tcgacggaaa tggcaccaat gcgtgtattc cagccagtcc ccaaagtgaa
     2041 cgcgtaataa ttattcgcga ccatcgagcc accatacagc gttaataagt tattaaaacc
     2101 atactgatga cccgcctgga caaaatcact ttgtttgctc gccccttcaa tatggctacg
     2161 acccgccgca aaatcatatt tcgacacgcc gggttgcagc atatttggta ccgctgcata
     2221 aggcaccaga taggtggtta ccgaaccgtc cgcctctttc acgctgacat caagatccgc
     2281 tccaccaccg gccaactgca aatctgtaat cgcaaacggg ccaggaggaa cctctttctg
     2341 ataaaccaca aaaccattct gttcaatagt taccagcgcg ttactctgag caatcccctg
     2401 cacccgtggc gtaaaatttt gtttcgagtt aggcaacatc tgcatatcac gaaacaaccg
     2461 cacaccgctg aagcgaacag aatcaaaaat atcgcttgat gtgtacatat cacccacacg
     2521 aagcgtgccg agaagttggg caaatccacg ttccagatac agggtattgc ttttccacac
     2581 ccctggattg ttatttgttt tactgaaact ggcatcagaa tgcagttgcc actcctgtaa
     2641 atttaacccg ctgttaaaac gtacatatgt actcttgcta ttaccggacg ctttataatc
     2701 gctgtaatac tgactcacat aataagaggt ataaaacgca ttaatacccc gctcccagtt
     2761 ttctggtgga acatagccac tttccagctc ttccacccag gcctgcggga cactgaaatc
     2821 gagacgaaaa actccgatat cccaggtata gctcccgccc tgaataagtt gcttaaatgt
     2881 taaacattgc ttaccgctgg caaagctatc ggtattaatg cctaaccgct tgatcatttc
     2941 ccttgataaa catgtttctt gcgggttgtc tttaacaatg atctcatatt tcccacgcca
     3001 ttgtttattg acataaatat cgatgtcata ctgccccggc aagggctgac tatcttcaag
     3061 acgaatattt gaaacctgct ggtctttcat tccacctatc atgaaatggg tatcaaaggt
     3121 ttcttcagct gcataagctt caatgccgat caataacgct acgattgctg atgcaagtgg
     3181 ggtcattctc aacataggca gctcctgcaa ttaaattttg tcactaatat aattgccatg
     3241 gtcatcgata atggtcagat gccagttatt tgcattatta cttttgacat taacactctg
     3301 actttctaag ggggcaatca taatagtttc ataattgact ttgacattat tagctttgac
     3361 atccgaaatc gtcacccaat tagctgagtc attttttatc accaatccat tgccactgcg
     3421 atttaccagt aactttttaa aggtcacttt atttaccgga gcaatacccg ctggccggta
     3481 aaacaactta attctgtttt gcatcgcaaa cttcagtgca ttcttacctt cttgttctgg
     3541 actatttggt ggaatatcca gaacattcag ataaaaaata ctctctttat tagtgggtag
     3601 tttattcggc ataattttaa tttttacttg ctgcccggaa tttgccccta tttttgccac
     3661 tggtggcgtt aacatgaaag gaacctgaat tttttctggt ggtaatgacg tatcgccatc
     3721 atcaatccac gcctgcatca gcgaagaacg gtttccctgg ttcatcaact gcaccatcac
     3781 ttctttgtgc tctgccgggt aaataatacg cgtcccgtag ataacaatac cggcatgtgc
     3841 aggaaggacc atagaagaaa tgagtaaagc taataatcct ttcatcttaa tgcctcttaa
     3901 attaaaaggc cgccatggag atagcggcca tgttaattac ctgaagaatc ttgcaatttt
     3961 atttattaat cctgcgccac acgcataatc agggaagata acacatcacc tgaattcact
     4021 gagttatctg tcagtttcag gtattttgct ttaaacggaa tggcatattg accgtccgct
     4081 gttttataac tctcgatgtt gaaaggtgca gaaacatcca ggacatcgct gttatcacct
     4141 aaatagagat aataacccac ggtatcgttg ctactggcta tcgccttatc gtttccggtt
     4201 attttatcgt tggtaatcaa cgagagtttt acaccagcaa cttctttcgg acagctaatt
     4261 tttaacatgc tgctggttac accgactgca ccatcatagg tcgagtattt agctttacct
     4321 tcaaagaccg tagttggtac agttggcata ttaatactta ctgaatcgcc acctgcttcg
     4381 aatttacacg gagattctgc aatattccct ttaatggtta atgttccggt atcaacgtct
     4441 gcggcaaata ccccagcgct cataaaaaaa gaagaaaaaa cagcggcagc aataattgaa
     4501 cgtttcataa tatgctcctt aataaaatct atttttggtt gttaagagtc tgtatctctt
     4561 ggaaacaaat ttctcacacc aaaaaa
//
LOCUS       DAZFRG010000323         4547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFRG010000323 DAZFRG010000000
VERSION     DAZFRG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.325"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..916)
                     /gene="htpX"
                     /locus_tag="ACHKUC_004621"
     CDS             complement(35..916)
                     /gene="htpX"
                     /locus_tag="ACHKUC_004621"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO6620726.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(1108..3156)
                     /gene="prc"
                     /locus_tag="ACHKUC_004622"
     CDS             complement(1108..3156)
                     /gene="prc"
                     /locus_tag="ACHKUC_004622"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO6620727.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(3176..3874)
                     /gene="proQ"
                     /locus_tag="ACHKUC_004623"
     CDS             complement(3176..3874)
                     /gene="proQ"
                     /locus_tag="ACHKUC_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO6620728.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(3971..4468)
                     /gene="msrC"
                     /locus_tag="ACHKUC_004624"
     CDS             complement(3971..4468)
                     /gene="msrC"
                     /locus_tag="ACHKUC_004624"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO6620729.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
BASE COUNT         1076 a         1241 c         1046 g         1184 t
ORIGIN      
        1 cgatcaggac gcgcttttta gtatttactt catattactt caggtattca cccgtacgca
       61 gagcttcaat acgtttatcc agcggcgggt gagtcatgaa caactcactg agcgatttcg
      121 acttcccgtt aatgcagaaa gccatcatgc tggttgcttc ttgcggttca tagctggttt
      181 tcaggcgttg cagcgcggca atcattttct cgcgaccaac cagttttgct gaacccgcat
      241 cggcgtggaa ttcacgatga cgcgagaacc acatggttat aatgctcgcc agaataccaa
      301 acaccagttc cagaaccgtt gcaaccgcaa agtagatcag cgggttgccg ttgctctctt
      361 caccttcatc acggtttccg cccataaaac ccgcggcaag ctgcgccaga atacgggaaa
      421 taaagataac gaaggtgttc accacgccct gaatcagcgt catggtgacc atatcaccat
      481 tggcgatgtg gctgatttcg tgagcaatta ccgcctcggc ttcatccggg ctcatgttct
      541 gcagcaaacc ggtgctgaca gcaaccagag aggcatcacg gcgcgcaccg gttgcaaaag
      601 cattgatgtc cggcgcatgg tagatagcca cttgcggcat agcgatcccc gcctgacgag
      661 cctgggttgc tacagtattg accagccaac gttccctttc gttacgcggt tgctcgatca
      721 cttccccgcc aacagatcgt aatgccatcc atttggacat cagaagcgaa acgaaggaac
      781 caccaaaacc gaacagcaag gccatgatca tcagcccctg aacgctgctc gactgtatcc
      841 ctgtcaggct cagtaccagc ccgaaaacga ccattacggc caggttcgtt agcaggaaga
      901 gcgcgattcg catcataatt ttctttttac ctcagtttaa caaaacgcaa tatgcgatac
      961 ccacatcgta tgggttacgc gactattttc aagtctggat agtgcgtaag tcaccagaaa
     1021 gacacaactt tacattttgt agcatctgat ttacggcatc ttgccgctgt taaaaaatca
     1081 ggcacaattt cttgtgcctg attgatatta cttgacggga gcgggttgtt ccgcgggtct
     1141 ggctttttca agcttcgcca gatcgagtgc gatattcacc gtctcatcca gataaggatc
     1201 tggctcctgg taatctttcg gtagatcatc cagtttcttc aactccggtt taccttcgcg
     1261 tttaaagcgt tcgttcaaac gcgccagacg cgtcgcatca tcttcattat tctctttctc
     1321 acgcacagcg taattcagag aaacgatatt gcgcttgtcc ttcatagcgt tgaagcgcgc
     1381 gatatccttc atgatgttct ggaactcagg atctttcgcg atacgcgcat tatgttcctt
     1441 cagcagctcc ggttcaaagg ccgttaaatc tcctgatttc acataagtcg cggcatcaat
     1501 gctatcccac ggcagcgcgt tatcttcgaa tttctcaccc gtttccgttt cttcattacc
     1561 cgtcggcatg atgatgtctg gcgttacgcc tttacgttgc gtactgccgc cgttaacgcg
     1621 atagaatttc tggatcgtgt actgcacaga acccagcgct ggccattcag gacgtaacat
     1681 ctgatcgtaa atacggttca atgaacggta ctgctgaacg gtgcctttac caaatgtcgg
     1741 ttcacccaca accagggcac gaccgtaatc ctgcattgcc gcggcaaaga tttctgaagc
     1801 cgaagcactg aagcggtcaa ccagcaccac cagcgggcct ttatagaaga cctgtccgtc
     1861 ggtgtcgctg tcttcacgaa ccttgccgtt gttatcgcgg acctgaacaa tcgggccgga
     1921 tggaatgaac agaccggaga gcgatacggc ttcagtcaac gccccaccac cattgctacg
     1981 caggtcgata atcacactgc tgacattctg tttttccagt ttctgcaatt gcactttgac
     2041 atcgtctgtc aaacctacat agaagcccgg aatatccagc acgccgactt tctctttacc
     2101 gacggtcttc accgacattt taaccgcgcg gtcttcgaga cgaatacgtt cacgggtcaa
     2161 cgttaccgta cgggtcttgg tccctttacc agcaggtaaa atttccagac gaactttact
     2221 gcccttaggc cctttaatta aggccaccac atcatcaaga cgccagccaa tcacgtcaac
     2281 catcggcttg cctgtttgac caacaccgac aattttgtca ccaacgctga tagctttgct
     2341 cttcgctgcc ggacctcctg ccaccatcga attgataacg gtgtagtcat catccatttg
     2401 cagcactgcg ccaatacctt ccagcgacaa actcatttca gtgttgaact gttcggtatt
     2461 acgcggggaa agatagttgg tatgcgggtc gatttcacgc gcaaacgccg tcattgccag
     2521 cgagaaaaca tcttcgctgt tggtttgcgc cagacgacga atggcaaatt tgtagcggcg
     2581 agtcagggtt tcacgaattt ccttatccgt ttttcctgtc agcttcaggc ttaactcgtc
     2641 gaatttgact ttactgtccc acagcgcgtt caactcagcc tcgtttttcg gccagggcgc
     2701 tttgctgcgg tcaaggttat aagtgtcgtt gccggtgaaa tccatcggct tttccagtac
     2761 cgacaaagcg tactggtaac gttcaaaacg gcgcttttgc gccagattgt agagatcgta
     2821 gaaaacgtcg agtttacctg aacgcagttc atcgcctaac tcagttttct ttttcgcgaa
     2881 ctgttcaaca tcgcttgcca gcagcacatt gtggctgtaa tcgagcagat tcaggtagcg
     2941 gtcaaagatt ttggccgaaa atgcctgatc gaggtcgaac tggcgataat gagaacgggt
     3001 gaagcgcgac gttacgcgct cactcaccgt cgcatgctgc gtctcttcct ttaataccgg
     3061 aatttgatca gcacgcgtga tatcttctac agcgaaggtc tggcctgcta ttgcaagcag
     3121 gccagctaac gcggtaagcc taaaaaacat gttcatgcct ggcccggcct ccgtttcaga
     3181 acaccaggtg ttctgcgcgc acaatcaaag acatacccga attcagctgg acgcggacgc
     3241 cgtctttggt gatttctaat acggtggcat ccatcgcgtt ttgacccgct ttcaccttaa
     3301 gggcttgtcc gacagtcaga gctgaaatgt cagaaaccgg ggtgtgctgt tcttcgcgag
     3361 gtgcttttac tgtttttggc gctttctcta ccggcttttg cgaacgaggt ttacgttcag
     3421 cgccttcttt gcggcgtggc gtggtcggac gtggcttgcg ttcgcggcgc ggtgcgtctt
     3481 ctttctcacc agcagctgcg gcagcttcgc gttttttcgc ttgctgttca gcacgctgtg
     3541 cctgaacacg cgctttcgct tcttcaagct gcttgcgagc atgctcgaca tgttgctcgt
     3601 ccagctcacc gcatgggttg ccgtcaagat cgacacgtgt tgcaccgggt ttaacaccgt
     3661 aaagataacg ccagctcgaa gtgtagagac gtaaagcgga tcgcaattgc gttttgctca
     3721 ggttcatttc cccggcaaca cgatcgacca aatcctgaaa aataccgatt ttcagcggac
     3781 gcgcttcacc ttccgcactg aaacagtggg gaaaacgttc ggccagaaac gcgattactt
     3841 ctttactgct attcaactta ggttgatttt ccatgaaatt tcctgattac aacggacgta
     3901 gccaacaagc gcaggcatga acaggcgtca ttataatgac gctatcagta aatgctacgt
     3961 tatccgttga ttatcctgcg acgctcgcaa agaatttttt gtaatccgtc gttgcaagca
     4021 ctttttcaag ctgtgccaca agctgacgta agccttgctc gtcctcgtct gtaaagcgac
     4081 cgaagacggt actatcgatg tcgagaacac caataatctg atttttcacc accagcggca
     4141 gaacaatttc agaattactc gccgcatcac aggcaatatg cccgtcaaac gcgtgaacat
     4201 cctcgatacg ctgcacttga ttgcgggcaa ccgcagtgcc gcatacaccg cgcccaacgg
     4261 gtatacggac acaggcaatt ttgccctgaa atggtccgag caccagtgta tcgtcctcaa
     4321 gcaaataaaa acctgcccag tttacgtcag tgagacgctc atataacaac gcactggtgt
     4381 tcgcaagcgt tgccagaaaa ctggtttctc ccgccatcag cgcgttaaag tcgcgattta
     4441 aatccgcgta aaattctgct ttgttcatta tataatcact tggttgtctt acctggaact
     4501 gccagcctat taaaataagc attaaatgcg ttaatgctca agatcat
//
LOCUS       DAZFRG010000324         4544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFRG010000324 DAZFRG010000000
VERSION     DAZFRG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.326"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..288)
                     /gene="chbB"
                     /locus_tag="ACHKUC_004625"
     CDS             complement(<1..288)
                     /gene="chbB"
                     /locus_tag="ACHKUC_004625"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO6620730.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAA"
     gene            complement(587..925)
                     /gene="osmE"
                     /locus_tag="ACHKUC_004626"
     CDS             complement(587..925)
                     /gene="osmE"
                     /locus_tag="ACHKUC_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO6620731.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1127..1954
                     /gene="nadE"
                     /locus_tag="ACHKUC_004627"
     CDS             1127..1954
                     /gene="nadE"
                     /locus_tag="ACHKUC_004627"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO6620732.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2184..3071
                     /gene="cho"
                     /locus_tag="ACHKUC_004628"
     CDS             2184..3071
                     /gene="cho"
                     /locus_tag="ACHKUC_004628"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO6620733.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWNGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNNQQAS"
     gene            complement(3031..3606)
                     /gene="ves"
                     /locus_tag="ACHKUC_004629"
     CDS             complement(3031..3606)
                     /gene="ves"
                     /locus_tag="ACHKUC_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO6620734.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGYSPDQVQ"
     gene            complement(3809..4294)
                     /gene="spy"
                     /locus_tag="ACHKUC_004630"
     CDS             complement(3809..4294)
                     /gene="spy"
                     /locus_tag="ACHKUC_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO6620735.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1141 a         1094 c         1095 g         1214 t
ORIGIN      
        1 cgcagcctta agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc
       61 aaccggtttg ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc
      121 taataacacg acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc
      181 aataatgacc ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga
      241 ggtagacatg cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc
      301 atcgacgatt atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg
      361 taacttgcga aaccaacatg actttttttg tggctgaaag gagtatacgg tataggcctg
      421 agcaatagta tttctttgac cttataaatt gtctctcctt gacctgtggt tatgaccctc
      481 ttcacatttc gggcaaataa ttcgcgaaga taattaagaa cacaggccaa aaaaaaccgg
      541 cgcaatggcc ggtttccgtt gttactcaag ctttcaggcg aagtgattac ttcgtagcct
      601 gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat
      661 catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct
      721 ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg
      781 caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt
      841 ctttggtacg atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca
      901 gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag
      961 cctggaatac attgggcgct ttttcaagcc cgtgaacgaa acggctcctc tttcagagga
     1021 ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac
     1081 gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca
     1141 acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg
     1201 tagtatcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct
     1261 cgggatcagc ggcggtcagg actccacgct tgccggtaag ctgtgccaga tggcgattaa
     1321 tgagctgcgc caggaaaccg gcaacgaatc actgcaattt attgccgtac gcctgcccta
     1381 tggtgttcag gccgacgaac aagactgcca ggatgccatc gcctttattc aaccggatcg
     1441 cgtattaacc gttaatatca agggcgcggt actcgccagc gagcaggcat tgcgggaagc
     1501 aggcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc
     1561 acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga
     1621 agctattacc ggattcttca ctaaatatgg tgacggcggt acggacatta atccgctgta
     1681 tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct
     1741 ttataagaaa gcgccaacgg ccgatcttga agatgatcgc ccttcactgc cagatgaagt
     1801 ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga
     1861 acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc
     1921 aattaccgtt ttcgatgatt tctggaagaa gtaataattt gcacatattg gtttgtgcga
     1981 aaaagagtac tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta
     2041 tgcaaattca atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt
     2101 gtaacctttg tcatcaaccc gcctcttttt taatcgcttc ccggctgtta tactggatag
     2161 ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg
     2221 aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca
     2281 cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca
     2341 aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca
     2401 tgctacggca atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc
     2461 tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca
     2521 atcgccagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag
     2581 aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt
     2641 tgcaagcatt gcagagcatc gctgatgaac aaaaactttg ttatggcctg ctgggactgg
     2701 aaccgttaag ccgcgggcgt gcttgttttc gttctgcatt aaaacgttgc gctggggcgt
     2761 gttgcggtaa agagagccat gaggaacatg cgctacgttt gcgccagtct ctggaacgtt
     2821 tgcgagtcgt ttgttggccg tggaatggtg cggtggcgct gaaagaacag cacccggaaa
     2881 tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag
     2941 aagcgacaac gttaattcgg gcacccgccg ggtttgatca cgacggttat aaaattcttt
     3001 gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggtg aataaccagc
     3061 aagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg
     3121 cctgccatcg aaccagcacg cgccctggtc ggtagtgagc agtttatcgc ccaattgcca
     3181 cgccccgtta atgacaaaca ctaccccgcc gcgtgagcca aaggtagtaa acgtacgttc
     3241 ggcaattctg actttcgcct tacagacatc cagtcgtgtc atgatgttga aatccatcga
     3301 catctgccct gccgtcagtt tcgcttttac caccaggtcc gctgcaaagg aaaaaggttg
     3361 taacggtttt aaggtatggt taaagcggtc tgcgctttca aggaacatct cgccgccttc
     3421 cagcaacgtc actatccttt ccataccggg aaacaaagaa aactccccat tcgccgcgat
     3481 ggatgtaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc
     3541 gcgcgtttca ccggcagcgt ttcgccacag attcaccgac attttacgca tatcaaagta
     3601 ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt
     3661 tatcggcaac tcgggtgatt tacgttagtg gcgatcaggc tttttttgca tctgtttgag
     3721 aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc
     3781 ggcggtctta cgttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg
     3841 ccgctggacg ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg
     3901 gcgtcaggat gttgtaaatc ttgttctggg tttccatgtg cgccagcatg ttagctttgc
     3961 gctgttcttc catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg
     4021 caatgatgtc atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct
     4081 ggcctttcat gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga
     4141 acatcatgtc ctcatgcgga ccgaacttgc ctttatggtg catcatcggc ttcgcgtcag
     4201 ccggtgctgc ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg
     4261 tagaggcaac aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt
     4321 tattacggct ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg
     4381 tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta
     4441 tggagaaaaa aagaaaattt gatggagagt gatgaaagaa tattacaaca cgatgatttt
     4501 gcagagatta tgaagaacta taccggatga ctggtgataa ataa
//
LOCUS       DAZFRG010000325         4455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFRG010000325 DAZFRG010000000
VERSION     DAZFRG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.327"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(480..653)
                     /locus_tag="ACHKUC_004631"
     CDS             complement(480..653)
                     /locus_tag="ACHKUC_004631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620736.1"
                     /translation="MNGFKNDMDDEKSLKGKGATEHSNKTPEMQPERFLAMQINNNAK
                     TYSLITQLNHGCE"
     gene            833..1573
                     /locus_tag="ACHKUC_004632"
     CDS             833..1573
                     /locus_tag="ACHKUC_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6620737.1"
                     /translation="MNQPIHNAYWLSRFDSLLDSALAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATSLRYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSLGDFSEPSSLATMIQEAWCSALHLEPWQGDGLVHFSRWTPSRKPA
                     SSDARPSSDDTPLSGGCSDTWKASDKKPGEAAVLWIKRGDVGALQKARNRASYLVKYE
                     TKLHNGSGQRNYGCSRGPGRLLDGRRSL"
     gene            2347..4395
                     /gene="ireA"
                     /locus_tag="ACHKUC_004633"
     CDS             2347..4395
                     /gene="ireA"
                     /locus_tag="ACHKUC_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO6620738.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLIKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNIKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSIKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYSNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWHVSANYTFTDSRRKSDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
BASE COUNT         1203 a          899 c         1073 g         1280 t
ORIGIN      
        1 ttaatgctgc tgtaaaacct ttctgtttaa gcaatcaatc tgctattcga ttttactgat
       61 gttacgttta acctttcctg agatttcgag tttcgacgtt agcttgagaa aagtatcttt
      121 acgctaaatc aacccgaact ttcctgaagt aatctgacgt tcttttcgat gtgcttacga
      181 cgattctgtt aaacgacttt ctggatgttt tgataacaat cagattgagg cattaacgat
      241 tcctgacgtt tttcttttcc gtgtaaggtc attctttttg tcatgaggtt tcttcttgca
      301 ttgcgaaagt gtgattatga acggttttga ttcagatgag atttacgtta tcgatggtta
      361 atcgttttta tggtgcgtag tgaaacgtaa cattttcggg atgagaaaat cgattctgat
      421 ggtgatggtg atggtgatga tcgtgttacg gcgttatctg taaacaggtt ttaaacgtgt
      481 cattcgcatc cgtgattgag ttgtgtgatt aagctgtagg ttttggcgtt gttgttgatt
      541 tgcattgcca ggaagcgttc tggttgcatt tctggtgttt tgttcgaatg ttctgtggct
      601 ccttttccct tcaggctttt ctcgtcgtcc atgtcgtttt taaatccatt catgctgttg
      661 ttgcgtattt tgctgctgtg tgcaggatta tcttttcgtt ccgggatggt ttgttccgtt
      721 tcaatcactc ttaatcagag agttgtagta ttgctcaatg ccggatgccg taaagtttta
      781 ctgcgttgat taatgatgtg aagtcaatcc aaatcaacgg agatctctca tcatgaatca
      841 accaatacac aatgcttact ggttatcccg ttttgacagc cttctcgaca gtgccctggc
      901 gcagcaccgt gccgtctcgt taatccgggt ggatttacgt ttccctgagt atatgcctgt
      961 caccatcatg gatcccgatc cggattcagc ggtgatttct cgtttttttg catccctgaa
     1021 agccaaaatt caggcttacc agcggaaaaa aagatgtgcc aatcagcgtg tgcatgcaac
     1081 cagcctgcgt tatttctggt gccgggagtt cggcaagatg tatggcagga aacattatca
     1141 cgtgatatta ctgctcaaca aagatacctg gtgctcgctc ggggatttca gcgaaccgtc
     1201 ttcgctggcg acgatgatcc aggaggcatg gtgcagcgcc ctgcatcttg agccctggca
     1261 aggcgatggc ctggtccatt tttccaggtg gacgccttcc cgtaaaccag catcgtctga
     1321 tgctcgccct tcttccgatg acacgccttt gtcgggtgga tgttctgata cctggaaggc
     1381 ttcagacaaa aagccgggtg aagccgctgt tctctggatc aagcgtggtg atgtgggagc
     1441 gctgcagaaa gcccggaaca gagccagtta tctcgtaaaa tatgaaacga agttgcataa
     1501 cggttccggg caacgtaatt atggttgcag tcgtgggccg ggacgtctac tggatggcag
     1561 gagaagcctg taaaacggca tccggagcct gagcttatgc cacagtaagg gcgtcagcca
     1621 acgcccttag ctcgttttct gaaaaagtcg tcctgaagtc atgtatctgc ttccattctc
     1681 atgttcatat ttcaccttac gctttcacta tctgttattg gaagtaccga tttattttgg
     1741 tgctgctcac ttacggtcat tcacggaaga ggatggtggt gaaccgtacg atcctggctg
     1801 ctggcgaggg aaggttcagg actgggatgg agcaggttta gtggtggaag atgagtcgga
     1861 atttctgact ggtcagtgag gtgatgatct ggtgtaactc aatctgctag ggtaaactct
     1921 ccttgctttt gcgcacaagc agtgggccct ggttcgctta tgtcctgcat ctacaagcga
     1981 atgactgacc ggagactccc atatctggtc agtcgcccat ttttattttc taccaaatca
     2041 gacaagcata ccgtaataaa taacgccagt cccctgtcgc tctcgtgtgc tctactggaa
     2101 tgttgttgat ggagatatcc agatgaaaga gcttacatct tcgacaggac tataaattat
     2161 gtagtaaaga gttccccgga gaaaggggct gttctgctgt tctcatcaag tctggcatgt
     2221 tcaggaaatt attcgaaaat attaactgaa tgagcgaaat gtgatttacc ttcttttctg
     2281 ttcattgtaa atttcctctt tgctaacgca aatcattatc attacgcctt tggcaaaggg
     2341 aaatttatga agaacaaata tattattgct ccgggcattg ccgtgatgtg ttctgcagtt
     2401 atatcatcag gttatgccag ttctgataaa aaagaagata cgcttgttgt tactgcctcc
     2461 gggttcactc agcagctcag aaatgccccg gccagtgtct cagtcattac ttcagaacaa
     2521 ctgcaaaaaa aaccggtttc agatctggtc gatgcagtaa aagatgttga agggattagt
     2581 atcactggtg ggaatgaaaa accggatatc agtatacgtg gtctaagtgg cgattacacg
     2641 ctgattctgg tcgatggacg acgtcagagc ggtcgggaat ccagaccaaa cggcagcggc
     2701 ggttttgaag ccggatttat ccctcctgtg gaagcaattg aacgcattga agtgatccgt
     2761 ggccctatgt cttccctgta tggttctgat gccatcggag gggtcattaa tatcataacc
     2821 aaaccagtta ataaccaaac atgggatggc gtacttggac ttggggggat tattcaggaa
     2881 catgggaaat ttggtaactc aaccacaaat gacttctatc tgtcaggccc attgattaag
     2941 gataaacttg gtcttcagct atatggagga atgaactatc gcaaggaaga tagtatctct
     3001 cagggaacac cggcaaaaga taataagaat ataacggcaa cgctccagtt tactccgact
     3061 gaaagccaga agtttgtttt tgaatatgga aaaaataacc aggtgcatac attaacacct
     3121 ggtgagtctc tcgatgcctg gactatgcgg ggaaatatta aacaaccaaa cagtaaaaga
     3181 gaaacgcata attcacgtag tcactgggta gcagcatgga atgcccaggg cgaaatactg
     3241 catcctgaaa ttgctgttta tcaggagaaa gttattcgtg aggttaaatc aggtaaaaaa
     3301 gataaatata atcattggga tcttaattac gagtcaagaa aaccggaaat aaccaacaca
     3361 atcatagatg caaaagtgac ggcatttctg ccggaaaatg tactgaccat cggaggtcaa
     3421 tttcagcatg cagagctccg tgatgactca gccacgggta aaaaaacgac agaaacacag
     3481 tctgtttcaa ttaaacagaa agctgttttt atagaaaatg aatatgcagc aacggattct
     3541 ctcgccctga ctggaggact gcgtctcgat aatcatgaaa tctatggcag ttactggaat
     3601 ccaagattgt acgctgttta taacctgacc gataatctca cactcaaagg ggggatcgca
     3661 aaagcatttc gggctccttc aattcgtgag gtgagtcctg gatttggaac actgacgcag
     3721 ggtggtgcct ctattatgta tggaaacagg gacctgaaac cggagaccag tgtaaccgaa
     3781 gagatcggta ttatttatag taatgatagt ggtttttcgg cgagcgcgac gctgtttaat
     3841 actgatttta aaaataagtt gaccagttac gatataggta caaaagatcc agtcaccggg
     3901 ttaaacactt ttatttatga taatgtaggt gaggcaaata tcagaggggt ggagcttgca
     3961 actcagattc ctgtgtatga taaatggcat gtatctgcaa actatacatt tactgactct
     4021 cgtcgaaaaa gtgatgacga aagtctcaat ggcaagtcgc tgaaagggga acctctggaa
     4081 agaactccca gacatgcagc caatgcaaaa ctggaatggg attacactca ggatattaca
     4141 ttttattcat ctctgaatta tacgggaaaa caaatctggg cagcacaaag aaatggtgct
     4201 aaggttcccc gcgttcgtaa tggattcaca tctatggata ttggtctaaa ttaccagatt
     4261 ctgccagaca cgctgattaa ttttgccgtt cttaacgtca cagacagaaa gagcgaggat
     4321 atcgatacca ttgatggtaa ctggcaggtc gatgaaggac gccgttattg ggctaatgta
     4381 agagtatcct tctgagagta tataaagaca acctgaggcc gtgagcctca ggtaccagtg
     4441 tagtaatcag taatg
//
LOCUS       DAZFRG010000326         4442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFRG010000326 DAZFRG010000000
VERSION     DAZFRG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.328"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(132..>4442)
                     /locus_tag="ACHKUC_004634"
     CDS             complement(132..>4442)
                     /locus_tag="ACHKUC_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6620739.1"
                     /translation="DSTGTEIAGNNATVTQEGELTVSSGGRGIDITGNNAKVDTKGKM
                     TITGTDSVGVSINGDSATLTNTGDIDVSNSATGFSLVTNEGIISLAGSMKVGDFSTGM
                     ALSGDNNSVTLAAKDINVTGQKATGVNISGESNTVDITGNILVDKDQTADNAVDYFYD
                     PSVGVNISGNSNTVSLDGKLTVIADSELTSRKYMEFDGSQENISGLTVSGDGNTVNLN
                     GGIQFVGEKNALADGSTIADKRSYFGKTPLVSVDGQSKVYLNGDSTISGSLPLGYANI
                     LQLSNKAALEIGSDATFSMQDISVYEHYFAQTPQIIKVDTGSQVVNNGDVDIWNISFA
                     GIWGENSTGINNGNITLSQYDYSSPETSFSEPDHMAFLSSSGGSAVNNGTITAKVMEQ
                     HSVLNLGSTAGVADPRVFNNSVSSMMGMEAYGKGTVLNSESGVIDMYGRGNIGMLAVD
                     DSAADNAGKITLDTLWVDQNDTTTLRTDLPSSTAIDYGVGMATGTNSGGGARSNGVAT
                     NQQGGVITVYNAGAAMAAYGASNMVINQGIINLEKNGNYDGSLGANMLVGMAVYNRGT
                     AINDKTGVININVDTGQAFYNDGTGTILNYGEINLLGSPMDSADSHMGAIPENLDLLT
                     ALTGSGETDMRTASSGGFVTTKALANYGNETLNSNVAAKAWLYNQDKANLTINGELSI
                     GQGLENSGLLNSDTISAAANVYNRASGSIITDQLSLTGSNSFFNEGNFSGSVAGSSYK
                     QNVVNTGTMAVMADGKSLISGSFLLYNEAGATLSNSSSAVSGGENAIVNVTRTGDSLA
                     QVNRGTITAVNGYSAIKTASTGSNSNGKWIWNTDTGVISGVNPNAPLIDLGRGYNFAN
                     AGTINVQGDGAVAISGGTTSYTVQLVNSGTINVGTAQGQADGTNGTGLIGIKGNGSDT
                     TINNAQSGVINVYADNSWAFGGKTKAIINNGEINLLCDTGCDIYAPGTTGTLNDHNST
                     TDIIVPAATSTPTQGSVPTVPADSSAQQKLTNYTIGTNSDGTSGMLKANNLVISDNVK
                     VNTGFSAGTADTTVVINDVFKGENISGAENISSSTVMWNAQGSTDASGNVDVTMTKNA
                     YTDVVTDSSVNNVAQVLDSGYTNNDLYTSLNVGTTAELNSALKQISGSQATTVFNEAR
                     VLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNNTQYDMMALRKSMTLTEYQNLS
                     LEYGIARLEGNGSDTVGDNGVTGGYSQFFGLKHQMAFDNGMSWNNALRYDVHNLDSSR
                     SIAYGDVNKTADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLEGGYQERN
                     AGDFNLSMNSGSETAVDSIVGLKLDYAGKEGWSANATLEGGPNLSYVKSQRTASISGA
                     GSQRFNIDDGQSGGGFNSLATMGVKYSSQESALQLDAFHWKEDGISDKGVMLNFKKTF
                     "
BASE COUNT         1028 a         1212 c          977 g         1225 t
ORIGIN      
        1 aacaagatta ctggatgagt agctctaata tcctgaaaga caccaaaaca aaacgcgtca
       61 agcaccgcat ccggcaataa aaaaaccgca acctgtatca gcgttgcggt ttatgtatta
      121 catgctaaaa attagaatgt tttcttgaag tttagcatca cacctttatc actgatgccg
      181 tcttctttcc agtggaaggc atctaactgc aatgcactct cctggctact gtatttcaca
      241 cccatcgtcg ccaggctgtt gaaaccaccg ccactctgac catcgtcaat gttgaaacgt
      301 tggcttccag cgcccgaaat actcgcagta cgttgacttt tcacatagct cagattcggc
      361 ccaccttcca gcgtggcgtt cgcactccag ccttctttcc ctgcatagtc cagtttcagc
      421 ccaacgatgc tgtccaccgc cgtttcgctg ccgctgttca tgctcaggtt aaagtcaccc
      481 gcattgcgtt cctgatagcc accttccagc gtatgacgca gtttcacacc cgcataaggc
      541 gtcacattca gaccttcacg cagttcaaag gtttttgccc cttcactacg gaattccaga
      601 tactgctgtt tcacattagc atcagcggtt ttgttgacgt cgccgtaagc tatcgagcga
      661 ctgctatcga gattatgcac atcataacgc agggcgttat tccagctcat accgttgtca
      721 aacgccatct ggtgtttcag accgaagaac tggctgtaac cgccagtcac accattatcg
      781 cctacggtat ctgaaccatt accctccaga cgcgcaatac cgtattccag actcagattc
      841 tgatattcag tcagtgtcat ggatttacgc agcgccatca tgtcgtactg tgtattattt
      901 cccagttcag cacgcgggtc gcctttcgct accacgttga aggccagacc gtttgcaact
      961 tccggtgctg catcggacag catgctgaag cggttactta acactcgtgc ttcattaaat
     1021 accgttgttg cctggctgcc gctaatttgt ttcagcgcac tgttcagttc agcagtcgtt
     1081 cctacgttca gactagtgta caggtcgtta ttggtgtaac ccgaatccag tacctgagcg
     1141 acattattaa ctgaagaatc cgtcacaaca tccgtatagg cgtttttggt catcgtgacg
     1201 tctacgttgc cgctggcatc agtgctgccc tgagcattcc acattaccgt ggaagaagaa
     1261 atattctcag ccccggaaat attttcacct ttgaaaacat cattaatcac aacagtcgta
     1321 tcggcagtac ctgcactgaa cccggtattt accttgacgt tgtcgctgat gacaaggtta
     1381 ttggctttca acataccaga ggtgccatcg ctattggtac caatagtgta gttagtcagt
     1441 ttttgctgtg cactggagtc cgcaggtact gtgggtactg acccctgcgt aggtgtgctc
     1501 gttgctgcag gaacgataat atcagtagta ctgttatgat cgttcagcgt accggtggta
     1561 cccggtgcat agatatcaca tcccgtatca cacagcaggt taatttcacc attgttaatg
     1621 atcgctttgg tctttccacc gaaggcccag gaattatctg cgtaaacgtt aattacaccg
     1681 ctttgtgcgt tattaattgt cgtatcactg ccattcccct tgataccaat taaccctgta
     1741 ccgttagtgc cgtccgcttg accttgcgcc gtaccgacat tgatggtccc actattcacc
     1801 agttgaaccg tatagctggt ggttcccccg ctgatagcga ctgcaccatc accctgcaca
     1861 ttaatagtgc cggcgttggc aaagttatag ccgcgaccaa gatcaattaa cggtgcattt
     1921 ggattcacgc cgctaattac acccgtgtcg gtattccaga tccattttcc gttgctgtta
     1981 gaacccgtac ttgccgtttt aattgcactg tagccgttga ccgcagtaat ggtgccacgg
     2041 ttaacttgcg cgagtgaatc gccggtgcgg gttacgttaa caatggcatt ttctccaccc
     2101 gaaacggcgc tgctgctgtt gctgagtgtt gcgcctgctt cgttataaag caggaagctg
     2161 ccactgataa gcgacttacc gtcagccatt acagccatcg ttcccgtgtt aaccacattt
     2221 tgcttatagc ttgagccagc aaccgaaccg ctgaagttgc cctcattgaa aaacgagttg
     2281 ctacccgtta gcgataactg atcagtgata atgctacccg atgctcggtt atagacattc
     2341 gcggctgcgc taatggtgtc agaattgagt aagccgctgt tttccagccc ttgcccgatg
     2401 gatagctctc cgttaattgt caggttggcc ttatcctggt tataaagcca cgctttggca
     2461 gccacattgc tattcagcgt ttcattgccg tagtttgcca gcgctttggt cgtcacaaac
     2521 ccgccacttg aagcagttcg catatccgtt tcgccgctgc ctgtcagtgc tgtcagcaga
     2581 tccagattct ccggaatagc ccccatgtga gagtctgcac tgtccattgg cgagccgaga
     2641 aggtttatct caccatagtt cagaattgtc cctgtaccgt cgttataaaa cgcctgcccc
     2701 gtatcgacgt taatattgat cacgcctgtt ttatcgttga tagcggtgcc tctgttatag
     2761 accgccattc ccacgagcat attcgcgccc aagctgccat cgtaatttcc gtttttctcc
     2821 agattgataa tgccctggtt aataaccatg ttgctggcac cgtaagcagc catagcagca
     2881 ccggcattat aaacagtgat tacaccgcct tgttggttag tcgcaacacc gttgcttctt
     2941 gcgccaccac cactattcgt tccggtggcc ataccaacac cgtaatctat agccgtgctg
     3001 gaagggaggt cagtacgcag cgtcgtggtg tcgttttgat cgacccaaag cgtatccagc
     3061 gtgattttcc cggcgttatc cgctgcggag tcatcaactg ccaacatacc aatgttgccg
     3121 cgcccgtaca tgtcaataac gccgctttcg ctgttaagaa cagtgccttt accgtaggcc
     3181 tccattccca tcatactcga cacgctatta ttgaataccc tcgggtctgc aacaccggct
     3241 gtagagccca gattgagaac actgtgctgt tccatcactt ttgcagtgat cgttccgttg
     3301 ttcacagcac taccgccact ggagcttaaa aaggccatgt ggtcaggttc tgagaacgat
     3361 gtctccggag aagaatagtc atattgagaa agcgtaatat ttccgttgtt aataccggta
     3421 gagttttcgc cccaaatgcc tgcaaaactg atattccaga tatccacatc accattgttg
     3481 acgacctgtg aacccgtatc aaccttgata atttgcggag tttgcgcgaa atagtgctcg
     3541 taaacactaa tgtcctgcat actgaacgta gcgtcactac caatttcaag cgccgcctta
     3601 ttgcttagct gaagaatgtt ggcatagcct aacggcaaag agccgctgat agtagaatca
     3661 ccgttcagat aaactttgga ttgtccatcg acactaacaa gaggtgtctt accaaagtag
     3721 ctgcgcttat cggcaatagt tgaaccgtct gccagcgcgt ttttctcgcc cacaaactga
     3781 atgccgccat tcaaattgac ggtgttacca tcgccagaca cggtaagccc agaaatattc
     3841 tcctggctac catcaaactc catgtatttg cgtgaggtta actcactatc tgcaataacg
     3901 gttagcttac cgtccagtga taccgtgtta ctgttgccgc tgatattcac accaacggat
     3961 gggtcgtaga aataatctac cgcgttatct gcggtttgat ctttatccac cagaatatta
     4021 ccggtgatat caacggtatt actctcgcct gagatgttaa cgcctgtcgc tttctgcccg
     4081 gtgacattga tatctttcgc cgccagcgtc acgctgttgt tgtcgccgct aagcgccatg
     4141 cccgttgaaa aatcgcccac tttcatgctg ccagccagcg agataatccc ctcattggta
     4201 acgaggctga atccggtagc cgaattgctg acgtcaatat cgcccgtatt ggtcaacgtc
     4261 gcactgtcgc cattgatgga cacaccaacc gagtcagtac ctgtgatggt cattttgcct
     4321 ttagtgtcga ccttggcatt gttgcctgta atgtcgatac cgcggccgcc gctgctaacc
     4381 gtgagttcac cttcctgagt aacggtggcg ttattacctg cgatctcggt gccggtggaa
     4441 tc
//
LOCUS       DAZFRG010000327         4441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFRG010000327 DAZFRG010000000
VERSION     DAZFRG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.329"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(251..496)
                     /gene="ymjA"
                     /locus_tag="ACHKUC_004635"
     CDS             complement(251..496)
                     /gene="ymjA"
                     /locus_tag="ACHKUC_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO6620740.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMATEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(630..737)
                     /locus_tag="ACHKUC_004636"
                     /pseudo
     CDS             complement(630..737)
                     /locus_tag="ACHKUC_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(923..1900)
                     /gene="pspF"
                     /locus_tag="ACHKUC_004637"
     CDS             complement(923..1900)
                     /gene="pspF"
                     /locus_tag="ACHKUC_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO6620741.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCSALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            2067..2735
                     /gene="pspA"
                     /locus_tag="ACHKUC_004638"
     CDS             2067..2735
                     /gene="pspA"
                     /locus_tag="ACHKUC_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO6620742.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2789..3013
                     /gene="pspB"
                     /locus_tag="ACHKUC_004639"
     CDS             2789..3013
                     /gene="pspB"
                     /locus_tag="ACHKUC_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO6620743.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELPQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            3013..3372
                     /gene="pspC"
                     /locus_tag="ACHKUC_004640"
     CDS             3013..3372
                     /gene="pspC"
                     /locus_tag="ACHKUC_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO6620744.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMVFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            3381..3602
                     /gene="pspD"
                     /locus_tag="ACHKUC_004641"
     CDS             3381..3602
                     /gene="pspD"
                     /locus_tag="ACHKUC_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO6620745.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            3677..3991
                     /gene="pspE"
                     /locus_tag="ACHKUC_004642"
     CDS             3677..3991
                     /gene="pspE"
                     /locus_tag="ACHKUC_004642"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO6620746.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            4201..>4441
                     /locus_tag="ACHKUC_004643"
     CDS             4201..>4441
                     /locus_tag="ACHKUC_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620747.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSI"
BASE COUNT         1178 a         1056 c         1113 g         1094 t
ORIGIN      
        1 cgtttgtgtt cagaggtcgt aatgagggcc gtgtggttgg tcattgaaca ggtggcgtcg
       61 ccatcagttc aacattcgta cccgtgattt ctttataact ataactcaca gaacaactta
      121 gcgaggagca gagcggtacg atcgtgagaa acccattaaa gcacgctgct gtgcgtgtca
      181 tttttagccg gatgcggcgc gaacgcttta tccgggcaac gatactgacc gatagtctgc
      241 aattaaataa ttactcatta ccccattgat tcaaaaactc tgcgatctca tcaatgcgta
      301 cgggattaat ccccgcttca gtagccattt catgttgggc ttcttcactg atctcttcat
      361 tgttcatcaa acgggtgagc agtaactgga agtagtgggc cagtggtgtg cgttcagcgt
      421 ctgcgacagg ctctgcgcat tcggtcgcgt actcatcggc aatatcaaaa tattttagcg
      481 ggatatcgtg gttcattatt tgcccctggg gttaatgcgc taagcataaa ggcggatgat
      541 agcactcgtg atccgggtca tacatatttt tatagttaat ggcgcggtgc attaccctca
      601 ggcaggataa tgcgccacgc attctactat tacgtttcgt tcaccggcgt tctgtcgcca
      661 tcatacaacg gcactttgcg atagcggcgg atcaaatacc acaaatatgc gccgcctaac
      721 gaagcccata ccatcccggt tgcgcaattc gatgcggcaa tgaaaatttt gcaggatgcg
      781 ctgagagatt aatacgcaga agcagggtta acgcaataat gcctgatgcg cagcgcttat
      841 caggcctgca aacgacgtat tgattatgta tgccggataa agcgttcacg ctgcatccgg
      901 caagttgtat tgcccaactt cgctaaatct ggtgcttttt caacaacgcg cggaactgat
      961 gataggttaa ccccagcaat tcagcggcgc gcttctggtt atacttccct tgttccaagc
     1021 tgacctgaag cagttctttt tcctgctgca tctgaaactc gcgtaaatcc agtggcagcg
     1081 tcggaagcga agtgttttct gaaacggcga tggcttcttc agatggacgg cgtttaaagg
     1141 gatcaataat gatgtcatca agcggataat cgctggtgcc gtggcgatac actgaacgtt
     1201 ccaccacgtt tttcaattca cgaatatttc ccggccagcg ataattcagc aatgtttctc
     1261 tggcgcgctc cgtaaacccc gggaacagag gcagcttgat ttctcgacac atctgaatgg
     1321 caaagtgttc tgccatcagc ataatgtcgc tttcgcgctc gcgcaggggc ggcagttgca
     1381 ctacatcgaa agccagccgg tcgagcaggt cagcgcgaaa agtaccttca ttgaccatcg
     1441 ccgggagatc ggcattcgtc gcgcatacca accgcacatt cacctgcaat ggctggctgc
     1501 cgccaacgcg ctccagttca ccgtactcaa tcacgcgcaa taatttctcc tgtaccatca
     1561 tcggtgccgt agcgagttca tcaagaaata gcgtaccgcc atcggcacgt tcaaatctcc
     1621 caggatgacg tttttgcgca ccggtaaacg cccccgcttc gtgaccaaac agttcggaat
     1681 ccagcagatt ttcatttaac gccgagcagt taagggaaat aaacggcccc tgccaacggg
     1741 aggagagata atgcagacgg ctggcaatca gctctttacc tgtgccgcgc tcgccgatga
     1801 tgagcaccgg tttgtccagc ggtgcgagat gcgaaacctg ttccagcact tcgagaaagc
     1861 tgtttgcctc accaagtaaa ttatctttgt attctgccat gatgaaattc gccacttatt
     1921 agtgtaattc gctaattcat cctggcatgt tgctgttgat tcttcaatca gatctttata
     1981 aatcaaaaag ataaaaaatt ggcacgcaaa ttgtattaat agttcagcag gacaatcctg
     2041 aacgcagaaa tcaagaggac aacattatgg gtattttttc tcgctttgcc gacatcgtga
     2101 atgccaacat caacgctctg ttagagaaag cggaagatcc acagaaactg gtgcgtctga
     2161 tgatccaaga gatggaagat acattggttg aagtacgttc tacttcggcg cgtgcgctgg
     2221 cagaaaagaa acagctgact cgtcgtattg aacaagcgtc ggcgcgtgaa attgaatggc
     2281 aggaaaaagc ggaactggca ctgctgaaag atagagaaga tctggcgcgt gcggcgttaa
     2341 ttgaaaaaca gaaactgacc gatctgatta agagcctgga acatgaagtg acgctggtgg
     2401 acgatacgct agtgcgtatg aagaaagaga tcggcgagct ggaaaacaaa ttgagcgaaa
     2461 cacgcgctcg ccagcaggca ttgatgttac gccatcaggc ggcaaactcg tcgcgcgatg
     2521 tgcgtcgtca gctggacagt ggcaaactgg atgaagcaat ggctcgtttc gaatctttcg
     2581 aacgccgtat cgaccagatg gaagcggaag cagaaagcca cagcttcggt aaacaaaaat
     2641 cgctggacga tcagtttgcc gaactgaaag ccgatgatgc aatcagcgag caactggcac
     2701 aattaaaagc caaaatgaag caagacaatc aataataaca tccaggcggc gtccgaacgc
     2761 gccgccgctc atcgtctaag gagtacttat gagcgcgcta tttctggcta ttccgttaac
     2821 catttttgtg ctgtttgttt taccgatctg gttatggctg cattacagca atcgttccgg
     2881 tcgcagtgaa ttgccgcaaa gtgagcagca gcgattagcg caactggctg atgaagcaaa
     2941 acggatgcgc gagcgtattc aggcgctgga atccattctt gatgcagaac atccgaactg
     3001 gagggatcgc taatggcggg cattaatctc aataaaaaat tatggcgtat tccgcagcag
     3061 ggcatggtcc gtggcgtctg cgccgggatt gccaactatt ttgatgtacc ggtaaaactg
     3121 gtgcgtatcc tggtggtgct gtcgattttc ttcggtctgg cgctgtttac cctggttgct
     3181 tacatcattt tgtcatttgc gcttgatcca atgccggaca acatggtctt tggtgagcag
     3241 ctaccttcca gcagcgaatt gctggatgaa gtcgaccgtg aactggcggc aagtgaaacg
     3301 cgtttacgcg agatggaacg ttatgtcact tccgacactt tcacgctgcg tagccgtttc
     3361 cgtcaactgt gaggaaagtt atgaatactc gctggcaaca ggccgggcaa aaggtgaagc
     3421 ctgggttcaa attagcaggc aagctggtac ttctaaccgc actgcgctat ggcccggcgg
     3481 gtgtggcggg ctgggcgata aaatcagttg ctcgccgacc gctgaaaatg ttgctggctg
     3541 tggcgctgga accgctgtta agtcgggctg ccaataaact ggcgcagcgt tataaaaggt
     3601 gaggggagtt tcgcaaaaat ggttaaatct caggcgtata atggatagca attttcatcc
     3661 atagaaggac gcttacatgt ttaaaaaagg cttactcgct ctgacactgg tgttttcact
     3721 gcccgttttc gccgctgaac actggatcga tgttcgtgtt ccagagcagt atcagcaaga
     3781 acacgttcag ggggccatca atattccctt gaaggaagtg aaagagcgca ttgccaccgc
     3841 cgttccggat aaaaacgaca ccgtgaaagt gtattgcaat gccggacgcc agtcagggca
     3901 ggcaaaagag atccttagcg agatgggata tacccacgtt gagaacgccg gtggcctgaa
     3961 agacatcgca atgccgaagg tcaaaggtta aaagaattca ccatgagcgg cgcttatgcg
     4021 ccgttttttc tgtcacaccc tatttacatc cccatagatt atttgcgtta gctcacaaat
     4081 acgctttttc ccaggtaaaa aaagatttcc tgcgtgacta aaccccttga gctcaattga
     4141 cagtttattt tatgcggagt agtctctcgt ttcatgggac cgctaccacg gaaaggcaac
     4201 atgaaacaga aaattacgga ttacctggac gaaatctacg gtggaacatt taccgcaact
     4261 catttacaga aacttgtaac gcgtcttgag agtgcgaaac gattaattac acagcgacgt
     4321 aaaaaacact gggatgaaag tgatgtcgtg ttaatcacct atgccgatca atttcacagt
     4381 aatgatttaa aaccactacc cacatttaat cagttttacc atcaatggct gcaaagcatt
     4441 t
//
LOCUS       DAZFRG010000328         4427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFRG010000328 DAZFRG010000000
VERSION     DAZFRG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.330"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..749
                     /locus_tag="ACHKUC_004644"
     CDS             51..749
                     /locus_tag="ACHKUC_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6620748.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(794..1693)
                     /gene="eamA"
                     /locus_tag="ACHKUC_004645"
     CDS             complement(794..1693)
                     /gene="eamA"
                     /locus_tag="ACHKUC_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO6620749.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1811..2965
                     /gene="ydeE"
                     /locus_tag="ACHKUC_004646"
     CDS             1811..2965
                     /gene="ydeE"
                     /locus_tag="ACHKUC_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO6620750.1"
                     /translation="MLRIFAMLTIGRGATLPFMTIYLSRQYSLSVDLIGYAMTIALTI
                     GVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVF
                     ATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICS
                     AFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCI
                     SQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIG
                     LVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLG
                     AAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQPALC"
     gene            3092..3187
                     /gene="mgtS"
                     /locus_tag="ACHKUC_004647"
     CDS             3092..3187
                     /gene="mgtS"
                     /locus_tag="ACHKUC_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO6620751.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3187..3291
                     /gene="mgtT"
                     /locus_tag="ACHKUC_004648"
     CDS             3187..3291
                     /gene="mgtT"
                     /locus_tag="ACHKUC_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO6620752.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3406..4296)
                     /gene="dgcZ"
                     /locus_tag="ACHKUC_004649"
     CDS             complement(3406..4296)
                     /gene="dgcZ"
                     /locus_tag="ACHKUC_004649"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6620753.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSSMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         1195 a         1057 c          961 g         1214 t
ORIGIN      
        1 ctaatcaggt ctcaactcct tgttttcaat ggctcttaaa aggtgcaaaa gtgatttttc
       61 aaaagattgc ccgcttactt aaatccgaga tcaatggcaa ttcatggcat gttggtgatt
      121 tgttgccgtc agaagcggaa ctcgctgttc gttataatgt ttcgcgtaat accctgcgta
      181 aggcattatc cctgctggaa ggcgaaggaa ttattcacag aaagcatggt tcaggaacat
      241 acattcaaaa aaagaatttt gttgcacaca ttgatcacat gaacagtttc agtgaaattg
      301 cacataaaag cggcaaagag gcaggaagtc agattatgaa atttgaagtg caggatgctt
      361 ctcctactat tgcgactgag ctgaatttag tgactggtga gcaggtttat tacataaaga
      421 gactgcgatt cattgaggat aatgcagcac aattagaaga aacgtggatg tcagtggcac
      481 gttttcctga tttaaccgta tcgcatatgc aaaaatccaa attttcgtat attgagaacg
      541 aatgcgggat caaaatcatt ggcacctttg aaactttctc cccgactttt cctaccccag
      601 aaatcgccag tattttacgg atcagcccac gggatcccat acttaaaatt cagacccagg
      661 ctgtggatag taactctatt ccgctggatt attcgttact ttacagcaat attttcgagt
      721 tccaggtaaa gtactttttc ccgcgataaa agcagacgca cagagtgctg gcatcacatt
      781 ctgtgcgtat ggtttagcct ctgaccttta ccgctttacg ccaccgcaag ccaaatacat
      841 tgatatacag cccggtcata atgagcaccg caccgaaaaa ttgcagaccc gttaagcgtt
      901 catccaacaa tagtgccgca cttgccagtc caactacagg caccagtaac gataacggtg
      961 caacccgcca ggtttcatag cgtcccagta acgtccccca gatcccataa ccaacaattg
     1021 tcgccacaaa cgccagatac atcagagaca agatggtggt catatcgata gtaaccagac
     1081 tgtgaatcat ggttgcggaa ccatcgagaa tcagcgaggc aacaaagaag ggaatgattg
     1141 ggattaatgc gctccagatt accagcgaca tcactgccgg acgcgttgcg tgcgacatga
     1201 tctttttatt gaagatgttg ccacacgccc aactaaatgc tgccgccagg gtcaacataa
     1261 agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc agtacgccaa
     1321 aaatcgctaa ggcgatcccc gccaattgtt tgccatgtag tcgttcaccg aaagtaaacg
     1381 cgccaagcac gatagtaaaa aacgcttgtg cctgtaacac caacgaggcc agtccagcag
     1441 gcataccaaa gttaatagcg cagaacagga aggcaaactg cgcaaaactg atggttaatc
     1501 cataccccag cagcaaattc agtggtactt tcggtcgtgc gacaaaaaag atggccggga
     1561 aagcaaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta tgaagcccca
     1621 ctttgatgac cacaaaattt agcccccata caaccactac cagtagcgcc aacaccccat
     1681 cttttcgcga cattctaccg cctctgaatt tcatcttttg taagcaatca acttagctga
     1741 atttactttt ctttaacagt tgattcgtta gtcaccgttt acgacggcat taatgcgcaa
     1801 ataagtcgct atacttcgga tttttgccat gctaaccatc ggacgcggcg ctacgctgcc
     1861 gtttatgacc atttacttga gtcgccagta cagcctgagt gtcgatctaa tcggttatgc
     1921 gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga
     1981 taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcta gcggttttat
     2041 tgccattcct ttagtgaata acgtgacgct ggtggtgctc ttttttgccc ttattaactg
     2101 cgcctactcc gtttttgcta ccgtgctgaa agcctggttt gccgacaatc tttcgtccac
     2161 cagcaaaacg aagattttct caatcaacta caccatgctc aacattggct ggaccatcgg
     2221 tccgccactc ggcacgctgt tggtgatgca gagcatcaat ctacccttct ggctggcagc
     2281 tatctgttcc gcgttcccca tgcttttcat tcaaatttgg gtaaagcgca gcgagaaaat
     2341 catcgccacg gaaacaggca gtgtctggtc gccgaaagtt ttattacaag ataaagcact
     2401 gttgtggttt acctgctctg gctttctggc ttcttttgtt agcggcgcat ttgcttcatg
     2461 catttcacaa tatgtgatgg tgattgctga tggtgatttt gccgaaaagg tggtcgcggt
     2521 tgttcttccg gtgaatgctg ccatggtggt tacgttgcaa tattccgtgg gccgccgcct
     2581 taacccggct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcatcgggct
     2641 ggtcggtttt attttttccg gcaacagcct gctattgtgg ggtatgtcag ctgcggtatt
     2701 tactgtcggt gaaatcattt atgcaccggg cgagtatatg ttgattgacc atattgcgcc
     2761 gccaggaatg aaagccagct atttttccgc ccagtcttta ggctggcttg gtgctgcgat
     2821 taacccatta gtgagtggca tagtgcttac cagtctgccg cctttctcgc tgtttatcat
     2881 cttagcgtta gtgatcgttg ttgcgtgggt gctgatgtta aaaggaattc gtgcaagacc
     2941 gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataagaattt aattcagcct
     3001 tcgtttaggt tacctctgct aatatctttc tcattgagat gaaaattaag gtaagcgagg
     3061 aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc
     3121 cgtactggga ataattttat tttctgggtt tctggccgcg tatttcagcc acaaatggga
     3181 tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag
     3241 gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca
     3301 tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc
     3361 gttgatacag tagcatcagt tttctcaatg aatgttaaac ggagcttaaa ctcggttaat
     3421 cacattttgt tcgtcaataa acatgcagcg attccttccg gtttgcttac cctcatacat
     3481 tgccctgtcc gctcttccaa tgaccacatc cagaggctct tcaggaaatg cacgactcac
     3541 accagcagtc acggtaatgt tgatatgccc ttcagaatgt gtgatggcat ggttatcgac
     3601 taactggcaa attctgacac ctgcacgaca tgcttcttca tcattagtcg ctttgacaat
     3661 aatgataaat tcttcgcccc cgtagcgata aaccgtttcg taatcgcgcg tccaactggc
     3721 taagtaagtt gcgagggtgc gtaatactac atcgccgatt aaatgcccgt aggtatcatt
     3781 aaccaattta aatcggtcta tatccaacaa cattaaataa agattcagag gttcagcatt
     3841 gcgtaactga tgatcgaagg attcatcaag aacccgacga cccggcaatc ccgtcagaac
     3901 atccatactg ctacggatcg tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa
     3961 agaaagcaac ccttcctgaa aagcgtcgaa atgcgcgtcc tgccagtgat tttcaacaat
     4021 agccagcatt aattcccgac cacagttatg catatgttga tgggcagaat ccattagccg
     4081 aacgtaaggt aattcatcgt tatcgagtgg ccccagatga tcaatccacc gaccaaactg
     4141 gcacagtcca taagaatggt tatccgttat ttctggctta ctggcatctc tcgcgaccac
     4201 gctgtgaaac atacttacca gccactggta gtgggcatcg atagccttat tgagatttaa
     4261 caagatggca tcaatttccg ttgtcttctt gatcattgcc actccttttt cacagttcct
     4321 tgtgcgcgct attctaacga gagaaaagca aaattacgtc aatattttca tagaaatccg
     4381 gagttatgag catctctgag ataacattgt gatttaaaac aaaatca
//
LOCUS       DAZFRG010000329         4323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFRG010000329 DAZFRG010000000
VERSION     DAZFRG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.331"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            104..879
                     /locus_tag="ACHKUC_004650"
                     /pseudo
     CDS             104..879
                     /locus_tag="ACHKUC_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1012..1585)
                     /locus_tag="ACHKUC_004651"
                     /pseudo
     CDS             complement(1012..1585)
                     /locus_tag="ACHKUC_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1639..2517)
                     /locus_tag="ACHKUC_004652"
     CDS             complement(1639..2517)
                     /locus_tag="ACHKUC_004652"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6620754.1"
                     /translation="MKLKMQDLRLFNIVFESDPGWILDFSNRTLSAFFDEELNIDIDD
                     ERYQKEGTSKAKRVRCLLKQVDRETALRVLGALWQYKTESMPEQAEQSRNDYLALISR
                     LENADTDEAKGVKPVQAWHGVDWHSLIAEMNEMKSLPPHPRGFRFEAWLAELFSIFKL
                     APRSSFRNTGEQIDGSFRLNDEFYLMEAKWHQKRTSAADLHVFEGKLSTKATWTRGVF
                     ISWMGFTPEGLTAFGKGKRVICVSGYDLYHSLNHRIPLPDLLDAKTRHAAETGEPYAE
                     FDRLYALKDKQFGTLK"
     gene            complement(2845..3576)
                     /locus_tag="ACHKUC_004653"
     CDS             complement(2845..3576)
                     /locus_tag="ACHKUC_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6620755.1"
                     /translation="MNQPIHNAYWLSRFESILNGALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRKKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPVPGGCSDTRKDSDKKSGGSAVLWVKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGLGRLLDGR"
BASE COUNT         1243 a         1041 c          990 g         1049 t
ORIGIN      
        1 aactttcccg gataggctag ccccctgaat ctccagtcaa ccaatatcac ttatttaagt
       61 gataatctta atattagttt ttagactggt cattggaaaa cagatgattg atgcctcagg
      121 accggagaaa ctcagacggc atactgcaca ggaaaagatc gctaatgttc agaagagctt
      181 tgaaccagga atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagttactt
      241 ctctggcgca agcaatacca ggagggaagt cttactgcgg tgactgctag ggaacagatc
      301 gttcctgcct ctgaacttgc tgctgccata aggcagatta aagaacgcca gcgcctgccc
      361 ggaacaaaac gatggaaaat gaactcatta aagaagccgt tgaatatgga cgtgcaaaaa
      421 gtggatagcg cacgctccct tattgccggg gtgatgcact gggcggtcac taccggcggc
      481 ttcaacagtg aaacagtaca ggacgtcatg ccaggagcgg tggaacgccg cttcggcaac
      541 gagcttccgg cgtctccagt ggagtggctg acagataatg gttcatgcta ccgggctaat
      601 gaaacacgcc agttcgctcg gatgttggga cttgaatcga agaacacggc ggtgcggagt
      661 ccggagagta acagaatagc agagagcttc gtgaaaacga taaagcgtga ctacatcgtg
      721 ttcatgccca agccagatgt taatgacggc attgcataac catgcagtag caatcgaaca
      781 ttacaatgca gcatagttct aagttatcgc tcgccgcagg tatatatgcg gcagcggatc
      841 agtaatgggt taaatgataa cagatgtctg gaagtatagg gagaaattcc ctgagggatt
      901 cccaaaaaat ttagtaaacc aatatcatat tggtataccg ttgttgtaaa atgttcagcc
      961 ctgcgtcaag tgacaaggtg tttagtatga gtggggaatg tcggaacctg tttaggattc
     1021 tgtgtaaatg ccttttttca gaagtgacag gcagtcaccg aactcgataa taaagcggct
     1081 cattgccata cacctgtcct tcaacgacac cgtccatttc tgggacgcag actggattgc
     1141 cagccacacc acttttttca ccgagtcgtc tgtcgggaac actttacgct ttttgatcgc
     1201 atggcggatc acactgttta gtgactcgat ggcattcgtc gtatagcgca ctttgcgaat
     1261 gtccgtcaga taagcgaaga acgtggtaag attcggtcag ttagcctgcc agctccgact
     1321 tatctagtgc ttgctagcct gtctcttccg tgggtgcctg attaaatctc tttcaggtcg
     1381 cgagtgacgg ctttgtagtc cttccatgac acgaagcgca ggctgttgcg caccatatgc
     1441 acgatgcata atggatgctg gcctccggat atactgtgtt gatggcatct ggaaagcctt
     1501 tcaggctatc tacacaggtg atgaggatat cgttcagacc gcggtttttc agttcagtca
     1561 gcacattgag ccagaacttc gaccctgtac acgattctgt gtgaaacaac ggggagcagg
     1621 tcatcgcttc ccaacccgtt atttcagcgt gccgaactgt ttgtctttca gtgcatatag
     1681 tcggtcgaac tcagcatagg gctctcccgt ttcagccgcg tgccgggttt tagcatccag
     1741 aaggtcgggt aaagggatcc tatgattcag tgagtggtaa agatcataac cactgacaca
     1801 gataacccgc ttccctttac caaaagccgt caggccttca ggcgtaaacc ccatccaact
     1861 gataaaaaca ccgcgtgtcc aggttgcctt ggtactgagc tttccttcaa atacatggag
     1921 gtcggcagcc gacgttcttt tctgatgcca ctttgcctcc atcaggtaaa attcatcatt
     1981 cagtcggaaa cttccgtcaa tctgttcgcc tgtatttcgg aaagacgaac ggggagcaag
     2041 cttaaagata ctgaacagtt cagcaagcca ggcttcaaac cgaaaaccac gtggatgtgg
     2101 cggcagagat ttcatctcgt tcatttcagc gataagtgaa tgccagtcaa caccatgcca
     2161 tgcctggaca ggcttaacgc ctttggcttc atcggtgtca gcgttttcta agcgggaaat
     2221 cagtgcaaga tagtcattac ggctttgttc agcctgttca ggcatggatt ccgttttata
     2281 ctgccagagc gcaccaagaa cccgcagagc ggtctcacga tcaacctgtt tcagcaagca
     2341 tctaactcgt ttggcttttg acgtcccctc tttctgataa cgttcatcat caatatcaat
     2401 gttcaattct tcatcgaaaa aagcagacaa ggtgcggtta gagaaatcca gtatccagcc
     2461 aggatcgctc tcgaacacaa tattaaacag cctcaaatcc tgcattttca gtttcacgcc
     2521 tacctccttt tttcacagta taacgggatg aaagtggggg aggaaatgag tcagaacctg
     2581 tacaccagca ttaggggatt ccgcgtctgg tacaaagcag actatgagaa gagagacgct
     2641 ataacatttg agtcctacca atcgatctgt tcatttctat gaatggcagt aaatgcaaat
     2701 aaccagaaca accaaaggtt tttcgactta tgtgtcacga acggaagtag atggcaatcc
     2761 attgcattac ttcaggacga cttttcagaa agcgagctaa gggcgttggc ataaactcag
     2821 gcatcggatg ctgttttaca gggttcacct gccatccagt agacgcccca gcccacgact
     2881 gcaaccataa ttacgttgac cagaaccgtc atgctgcttc gtctcatact tcacgagata
     2941 acgggctctc tccatggctt tctgcatcgc ttccacatca ccacgcttga cccagagcac
     3001 agcggatcca ccagattttt tgtcagaatc cttccgggta tcagaacatc caccaggcac
     3061 aggcgtatca tcggaagaag ggcgagcatc agatgatgtc ggtttacgga aaggcgtccg
     3121 cctggaaaaa tgaaccagtc cattaccctg ccagggctca agatgcagag cactgcacca
     3181 tgccagttgg atcagcgtcg ccagcgaaga aggaacggtg aaatcccctg gcgagcacca
     3241 ggtatctttg ttgagcagta atatcacgtg ataatgtttc ctgccttttt ccttgccaaa
     3301 ctcccgacac cagaaataac gcagagtggt tgcacgcaca cgcttgttgg cacgtcgttt
     3361 tttccgctgg taagcctgaa tttttgcttt cagggatgca aaaaaacgag aaatcaccgc
     3421 tgaatctaaa tcggtatcca tgatggtggc aggcatatac tcagggaaac gtaaatccac
     3481 ccggattaac gagacggcac ggtgttgcgc cagggcaccg ttgagaatac tttcaaaacg
     3541 ggataaccag taagcattgt gtattggttg attcatgatg agagatctcc gttgatttgg
     3601 attgacttca catcattcat caacgcggta aaactttacg gcatccggca ttgagcaatg
     3661 ctacaacttt ctgattaaga gtgattgaaa cggaacaaac catcccggaa caaaaagata
     3721 atcctacaca cagggtaaaa acactcaaga acagcgaatg gatttaaaaa cgacatggac
     3781 aacgagaaaa gtcagaaggg aaaaggatcc acagaacatt caaacaaaac accagaaacg
     3841 caaccagaac gcttcctggt aatgcaaatc aacaacaacg ccaaaaccta cagcttaatc
     3901 acacaactta atcacggatg cgaatgacat gtttaaaacc tgtttacaga taacgccgtt
     3961 acacgatcat ccccatcaga atcgattttc ccttcctgaa agcgttacgt ttcactacgc
     4021 accataaaaa cgattaacca tcgataacgt aaacttcatc tgaatcaaaa caccaaaacc
     4081 gttcatcaca cttttgcaat gcaagaagag tcacatcatg acgaaaacaa tgaccttaca
     4141 cggaaaagaa aaacgtcagg aatcgttaat gcctaaatct gattgttatc aaaacatcca
     4201 gaaagtcgtt taacagaatc gtcgtaagca catcgaaaag aacgtcagat tacttcagga
     4261 aagttcgggt tgatttagcg taaagatact tttctcatgc taacgtcgaa actcgaaata
     4321 tca
//
LOCUS       DAZFRG010000330         4239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFRG010000330 DAZFRG010000000
VERSION     DAZFRG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.332"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..827)
                     /locus_tag="ACHKUC_004654"
     CDS             complement(<1..827)
                     /locus_tag="ACHKUC_004654"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HFO6620756.1"
                     /translation="MGIHRYYYRTMLFLACSFFFATGIASFNMGHPEILYFSAISAAL
                     SPFFAWCLRYPDEEINEGIWGYNAVLYGIACGMVVPVSVSGIAVLIVGSLGIVLFTPL
                     LSSVLRGLPVLTAPFIIATWLLHSGIMPHDMSVPSGEGAGSVLMKNYPVAFSGSFMEI
                     FMFPDAVGGILVMLGLLAGSRKLLMITVVASFLSVVVSFCIPELSPDKISAGLYGYNA
                     ILTVVAVYAFAGDNQFNNIFLGCGAIILAFIIPVLIGPRIPVPLLTSPFVISIWLYIL
                     "
     gene            complement(1187..2623)
                     /gene="hlyD"
                     /locus_tag="ACHKUC_004655"
     CDS             complement(1187..2623)
                     /gene="hlyD"
                     /locus_tag="ACHKUC_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin T1SS ABC transporter subunit
                     HlyD"
                     /protein_id="HFO6620757.1"
                     /translation="MKTWLMGFSEFLLRYKLVWSETWKIRKQLDTPVREKDENEFLPA
                     HLELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTLSGRSKEIKPI
                     ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQASLEQIRYQILSRSI
                     ELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLT
                     ILARINRYENLSRVEKSRLDDFRSLLHKQAIAKHAVLEQENKYVEAANELRVYKSQLE
                     QIESEILSAKEEYQLVTQLFKNEILDKLRQTTDSIELLTLELEKNEERQQASVIRAPV
                     SGKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEA
                     FPYTRYGYLVGKVKNINLDAIEDQKLGLVFNVIVSVEENDLSTGNKHIPLSSGMAVTA
                     EIKTGMRSVISYLLSPLEESVTESLHER"
     gene            complement(2642..>4239)
                     /locus_tag="ACHKUC_004656"
     CDS             complement(2642..>4239)
                     /locus_tag="ACHKUC_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO6620758.1"
                     /translation="VVMDKVLVHRGFSTLNVITVALSVVVVFEIILSGLRTYIFAHST
                     SRIDVELGAKLFRHLLALPISYFESRRVGDTVARVRELDQIRNFLTGQALTSVLDLLF
                     SFIFFAVMWYYSPKLTLVILFSLPCYAAWSVFISPILRRRLDDKFSRNADNQSFLVES
                     VTAINTIKAMAVSPQMTNIWDKQLAGYVAAGFKVTVLATIGQQGIQLIQKTVMIINLW
                     LGAHLVISGDLSIGQLIAFNMLAGQIVAPVIRLAQIWQDFQQVGISVTRLGDVLNSPT
                     ESYHGKLALPEINGDITFRNIRFRYKPDSPVILDNINLSIKQGEVIGIVGRSGSGKST
                     LTKLIQRFYIPENGQVLIDGHDLALADPNWLRRQVGVVLQDNVLLNRSIIDNISLANP
                     GMSVEKVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNNPKI
                     LIFDEATSALDYESEHVIMRNMHKICKGRTVIIIAHRLSTVKNADRIIVMEKGKIVEQ
                     GKHKELLSEPESLYSYLYQLQSD"
BASE COUNT         1298 a          998 c          759 g         1184 t
ORIGIN      
        1 agtatgtata accatatact tataacaaaa ggagacgtaa gaaggggaac gggtatccgc
       61 gggccaatca aaacaggtat aatgaacgcc agtataattg ctccgcaacc cagaaatata
      121 ttattgaact ggttatctcc tgcgaatgca tatactgcaa cgaccgttaa aatggcatta
      181 tatccataaa gcccggcaga gattttatca ggagaaagct caggaataca gaatgatacc
      241 accacactca gaaacgaagc gacaaccgta atcatcagta gtttccggct ccctgcaagt
      301 agtcccagca taacaagaat accgccgaca gcatcaggaa acataaaaat ctccataaag
      361 ctaccagaaa atgccaccgg atagtttttc atcaaaacag aacctgcacc ttcgcccgaa
      421 ggtactgaca tatcatgagg cattattccg gaatgtaata accacgtagc gataataaag
      481 ggggcggtca atacgggtaa ccctctgagc actgacgaca acaggggagt aaacaaaaca
      541 ataccaagac ttccgacgat aagtacagca ataccggaga ctgacacggg gacaaccatg
      601 ccacaggcta tgccatacag aacagcatta tatccccata taccttcatt aatctcctca
      661 tcaggatacc gcaaacacca ggcaaagaac ggagaaagtg ctgcactgat ggctgagaaa
      721 tacagtattt cggggtgccc catattaaaa gaggctattc cagttgcaaa aaaaaaagaa
      781 caagccagaa acaacattgt tctgtaataa tacctgtgaa tacccattac caaaggaggt
      841 tatcacctgt tttattttca tgaaaaatgt cacacacacc tcatacataa accattctcc
      901 gcttctgcgg gacagtaccg cccctgactc cacctcacag cggattgtgt atttttaaac
      961 aatcacagtc ttctcatata ctttccattc tgaagcttac ctcttcctct gtgataagct
     1021 tccgtcgcgg gatgtgttat acgccctgta agacagttat aaaggacatc aatgccatag
     1081 ttaatgacta ccgaattccg gtggatagtc agtagtggtt tgccacaaaa cagtgcagtc
     1141 acacatgaca ggagaagata tgagccgggt accgcggctc tgaaacttaa cgctcatgta
     1201 aactttctgt tacagactct tccaggggac taagaagata actgattacg cttcgcattc
     1261 cagtctttat ttctgcagtg acagccatac ccgagcttaa tggaatgtgc ttattcccgg
     1321 ttgacaaatc attctcttca acagaaacaa tgacattaaa aacgagtccc agtttctggt
     1381 cttctattgc atctaaattt atatttttca ccttacccac cagataacca tatcgggtgt
     1441 aaggaaaggc ctccacttta atgatggcat tctgcccgac gttaataaaa ccaatatctt
     1501 tattttgtac cagagcagta acctccagcg tgtcatcttc cggaacgatg accatcagtg
     1561 tttccgctgt tgtaacaacc ccaccttcag tatgaacctt cagttgctga acttttcccg
     1621 aaacaggggc tctgattact gaagcctgtt gacgctcttc atttttctct aactccagag
     1681 ttaataactc aatgctgtct gttgtttgtc ttagcttgtc taaaatttca tttttaaaaa
     1741 gctgcgtgac aagctgatat tcttcttttg cagacaatat ctcactctca atttgctcca
     1801 gttgcgattt ataaacccgt aattcatttg ctgcctcgac atatttattc tcctgctcaa
     1861 gtacagcatg ttttgcaatt gcctgtttat gcaataaact cctgaaatca tccagacggc
     1921 ttttttcaac tctcgataaa ttttcataac ggtttatacg ggcaagtatt gttaatcgct
     1981 ctgctctttt cttatccaga ttcagttctt tttgatactt ctgattttgc catgtggaaa
     2041 actgttcttt tatcaaagaa gttaaacgca gtacttcctc ttcagataca ttctgaaaat
     2101 aaggctcatc aggaagcttc agttcaggaa gtttatttaa ttcaattgac cggctcagaa
     2161 tttgataccg aatttgttcc agactggcct gtaacagtga tgactgcgtt tttaacgtat
     2221 cagcttcagc tcccagtgct gtaagcttta ataacacatc ccctttccgg actgactctc
     2281 cttcttttac gataatttct ttaactatcg agttttcaat aggtttaatt tctttgctac
     2341 gcccactgag tgttaatttc ccatttgcag tggcaacaat ttccacctgg cctaaaacag
     2401 ataaaatgaa agcaataacc agaaacccca taataaaata agcaaccaga cgcggccgtc
     2461 tggataccgg cgtttcaatt aattccagat gagcgggtaa gaattcattt tcgtcctttt
     2521 cacgtaccgg agtatctaac tgcttccgga ttttccatgt ttcactccag acaagtttat
     2581 agcgcaacag gaactcgctg aaccccatta accatgtttt catattcttc tgttctttct
     2641 gttagtctga ctgtaactga tataagtaac tgtataaact ttctggttca gaaagcagct
     2701 ccttatgttt accctgttca acaattttcc ctttttccat gacaataatg cggtctgcat
     2761 tttttactgt agacagacga tgagcaatga ttataaccgt tctgccctta catattttgt
     2821 gcatattgcg catgatgaca tgctccgact cataatccag agcactggtt gcttcatcaa
     2881 aaatgagtat tttagggttg ttcaccagcg cccttgcaat tgcgatgcgt tgacgttgac
     2941 ctccggataa tcctgccccc tgttccccga caatggtgtt atacccctca cgcaattcag
     3001 aaataaaatc atgagcacct gctaatttcg ctgcataaat aactttttcg acggacatgc
     3061 caggattagc cagtgaaata ttatcaataa tactgcgatt aagcagcaca ttgtcctgca
     3121 acacaacccc cacctgacga cgtaaccagt taggatcggc caacgcaaga tcatgtccat
     3181 caattaagac ctggccattt tcaggaatat aaaaacgttg aattaattta gttaatgtgc
     3241 tttttcctga accagaacgt ccgacaatac caataacctc cccctgctta atactgagat
     3301 tgatattatc taaaataacc ggagagtcag gcttatagcg aaaccggata ttacgaaaag
     3361 tgatatcacc attaatttcc ggtaatgcca gtttcccatg ataactttca gttggagagt
     3421 taagcacatc accaagtcgg gtaactgata taccaacctg ctggaaatcc tgccagattt
     3481 gtgcaaggcg aataaccggt gcaacaatct gacctgcaag catattaaaa gcaattaact
     3541 gaccaatact taaatccccg gaaataacca ggtgtgctcc caaccacaag ttgatgatca
     3601 taacagtctt ttgtattaac tgtattcctt gttgaccaat ggtggctaat actgtcactt
     3661 taaagcctgc agcaacatat cctgccaatt gtttgtccca tatgttcgtc atctgaggtg
     3721 agactgccat agcttttata gtgttaatcg ccgtgactga ttccaccagg aaagattgat
     3781 tatccgcatt ccgtgaaaac ttatcatcaa ggcgacgtcg caaaatgggg ctaataaaaa
     3841 cagaccatgc agcataacag ggcagcgaaa ataagatcac cagagtaagc tttgggctgt
     3901 aataccacat taccgcaaaa aatatgaatg aaaataataa gtccagaaca gatgttaatg
     3961 cctgtcctgt cagaaaatta cggatctggt ctaattctct taccctggca acagtatcac
     4021 caacacgacg actctcaaaa taagagatcg gtagcgccag taaatgccgg aagagtttgg
     4081 cacccaactc aacatcaatc cgacttgtac tatgtgcaaa aatgtaagtt cttaaaccgc
     4141 tgagtataat ctcaaacacc accacaacag ataatgcgac agtaataaca ttaagggttg
     4201 aaaaccccct gtgtactaat actttgtcca taaccacct
//
LOCUS       DAZFRG010000331         4224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFRG010000331 DAZFRG010000000
VERSION     DAZFRG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.333"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..994)
                     /gene="metF"
                     /locus_tag="ACHKUC_004657"
     CDS             complement(104..994)
                     /gene="metF"
                     /locus_tag="ACHKUC_004657"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO6620759.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(1376..2929)
                     /locus_tag="ACHKUC_004658"
     CDS             complement(1376..2929)
                     /locus_tag="ACHKUC_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6620760.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLVADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3062..4186
                     /locus_tag="ACHKUC_004659"
     CDS             3062..4186
                     /locus_tag="ACHKUC_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO6620761.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTYAIEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKKYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGASLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
BASE COUNT         1098 a         1059 c          961 g         1106 t
ORIGIN      
        1 acaaggctga taaccttatt ttcccgccct catttcgagg cagcattttg tgctctgttt
       61 aaaatttgtg atcactgtgt gattttcaca aaaggcacac tatttataaa ccaggtcgaa
      121 cacccagtgt atggcaaatc gcgtaactca tttcagcgcg gttaagcgta tagaagtgga
      181 aatctttcac tccttcacgg cttaaaatct tcaccatatc catggcaata ttcgcgccca
      241 ccagcttgcg ggtttcagca tcgtcatcca ggccgtcgaa catttgtgcc atccacgccg
      301 gaatacgcac gttggtcatg tcggcaaatt tcttcgcctg tttaaagttc gaaaccggca
      361 aaattcccgg aatgatttcc acatcaatgc ctgccgagac gcagcgatcg cgaaaacgca
      421 ggtagctttc gacatcgaag aagaactgag taatggcgcg gttggctccg gcatccactt
      481 ttcgtctcag gttgagtaaa tcggcctggg cacttttcgc ttctggatgt acttccggat
      541 acgccgccac ggagatatcg aaatctgcca cttcttttaa cagcgtcacc aggtcagaag
      601 catacatttc tggcttacca cttcccggcg gcaggtcgcc acgcagcgcc acgatatggc
      661 gaataccgtt attccagtag tcgcgtgcaa tgatccgcag ctcggcaggc gtcgcatcaa
      721 tgcaggtaag atgcggtgcc gcttccaggc cggtgcgatc tttaatgcct ttaataatgc
      781 tgtgcgtacg gtcgcgctcg ccggagtttg cgccataggt caccgataca aacttcggtt
      841 tcaggctgct aaggcgatcg atggagttcc acagggtctg ctccatttca ctggtacgcg
      901 gcgggaaaaa ttcgaacgaa acgttaatct gcccctggac ttctgccaga ctctgattca
      961 gggcatcccg ctggctggcg tgaaaaaagc tcatacctta cctcatcaat cgcttatcgt
     1021 tatatgttgt gttgtgcaca tctatccgtt tagacgtcca gatgtaaaga tgaaggaaaa
     1081 gccgaagggc gtcaaccgaa aaataaccaa tgatggtgag gaaaaatcag ggaagatgaa
     1141 aaaaactcat cttaattaga ggcgataaaa gatttctcta tagcgcaatc cacctcgcac
     1201 gttttgactc atattctgac aacacagcga cacctgggct aattcgaaaa acaacgtcct
     1261 gccaccggat ggtgatgtta aggcatctga ttcggcctgc gccctcgcgt aggccgaata
     1321 caggatctgc gcaatcaacg ggtaacaaac agttgagtca attcagtgcg cacttttatt
     1381 tgatatcctt cacgcgcata ccgttgagct gttcatccgt aatggtgttg cccgctttga
     1441 agtaatcaac caccgcgtga taaacgtaat aaccgcccgt tgtgttacgc gctttccctt
     1501 cggtaaaggc ggtaaaacca tctccaccat cagcaaggaa actctgagta gcaatgtgat
     1561 aaaccgtcgc atcttcaatg ggtttgccat tcagagtcag tgtgattacc cgctgaccaa
     1621 ccggcttgct gctgtcgtac ttcatttcca ggcctttcga tacctgtaaa acgccattgc
     1681 ttaaacttgc gccgtgttcc atcagactgc gtaattgttt acccgtgagt tccatcgtca
     1741 ccagttcgtt ggggaacggg aacgtactga taacgccgcc catcgtgatc gcccctgcgg
     1801 ggatctcatt gcggatacca ccagagtttg ttaacgccag ttgggtattt ttacccgctg
     1861 ctgccagtaa agcgtcagcc accaggttcc cgagggaagc cgattcacca taggcacgtt
     1921 ttaattcaac cggcgattgc gccaccgttt gttgcaccac ttcatcaagc tttttattcc
     1981 agccgtcgat cacctgtttc gtttgcggat cgggcttcca ctcgtcagcg taaatggttt
     2041 taagctcgaa gtttttcacc gtaaaattat gcggcttctc tttgtagtcg agaaccagtt
     2101 tgcccacatc aatcccgccg ctgtcagtgg agaggatgag cgtattgccg actttaatcg
     2161 gttccggcgt acccacatgt gcatgtccgg tgatcaaaat atccagccct ttcacctgac
     2221 ttgccgtctg aatatcttta tccaacgcgc gacgcacatc tgtgcctccc atactggact
     2281 ggcgggctgg aacaccttcg tggatcaggg cgacggtcaa atcaaccttg cctttgagtt
     2341 catcgatata acgttgtagc catttaattt catcgcgcgc ttcaataccc acgcgcgtag
     2401 cagcagagac agtgtcatta aaggcaaata ctccgtgcaa accaataacg cctattttca
     2461 caccgtcttt ttcgatgatg gtatatggct tatcccagaa tgatttactg ctgttctgat
     2521 aaaaaatatt gccctgcaca atagggaatt ttgcctggct caactgtaat aatgtgttgt
     2581 cccagccgtg gtcaaattca tggttgccta ttgtgacggc atcaaacggc atagtattca
     2641 taatatcgat aatagcctta cctttggtca ggctgctgat atatggcccg gtaaaatagt
     2701 cgcccgcatc aaaaaaccat gtcgctttat tttttgcttt ttcttgctta accagcgtag
     2761 tgatatttgc ccagccgcca atatcccgtt taccgtcagc gatccacggc actttataag
     2821 gttctacatg cgcatggagg tcgttggtat aaataatagt gacatcttta gcgcaagccc
     2881 agaacggcag tgttaatgcg atacccgcag caagtatttt aattttcata aacaatccct
     2941 ttcagtgaga taaaggattg ctacattacg ttctgaaaga cttatttatg tgagagtaat
     3001 cacatccgca ggggatagtt gccggacaaa atcaacgacc ttgtgaaatc gttggtgata
     3061 tatgtcgact ctgtttacga ttaatgcctg taaaacgttc ggttgccgaa atttgggatt
     3121 agcctcatcg gaagattaca gttggccaga ctataaactg ggttatccgg cactgcactg
     3181 tcgggcatgt ggcagttacc cgccgttgtt tgacgagcaa caatttcgtg actggttgtc
     3241 agttcacttg tctacttatg caattgagaa aggccatttc tgcccggtat gttatggcac
     3301 agacatgatt tgttatggtc acaatccaca gggttcgcaa cgcattcaat gccgtaactg
     3361 caaaaaagtc tggacgccta aaaaatatca gaaagaaatt acgcatcccc aggccattga
     3421 aaccgttcaa ctgttcatac cctttcaagg ggccagcgca gtacaaaaac tgtatgtttt
     3481 agtcagtctt gacgccactc gtggcaacat tcttcatctc tccacgaatt atacgcaaca
     3541 tcagacagga gacagtctgc ggtacagtta caagggcaac acagaaccaa ctatgcacca
     3601 tcgcgatatc gtgcagaagg tagatatgcg tgaagcgcaa ttcttgcgcc ggagccagtt
     3661 cgatgaaatt cagtatggca gtgctgtact caagcgcaac ggtaagggag ccatactacg
     3721 cccggttatc acggcacacg ggcatttcag aatactgaaa atccgctatc cagatgtcaa
     3781 aacgcatatt atttcacacg aatgttttct aagaggcgca attattacag cctgggcaga
     3841 tcagttccgc caacaacaag gtgaactttg gttcgtagaa gaagaaatca gtgacagtaa
     3901 cgctgatacg ccctggcatt ttaagggaac gacataccat ggttggtggc aaaatcagtg
     3961 gcagcgctgg gagcagggga ataactgcaa gatggtctgc ctactcacgg gagccagctt
     4021 agaaaggggc gcaaatgttt ctctggcaac cagtcgttgt tttatcacat ggctgacaga
     4081 ccaacacgac tttacccaaa gcgcgttatt atccgcaggt cgcgtaacgc agatgctgac
     4141 ctcactggcg ttaaaataca atgaatcgct cactccatct tgttagtcaa tacactcgac
     4201 aaacgaccat aaattaagag agat
//
LOCUS       DAZFRG010000332         4221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFRG010000332 DAZFRG010000000
VERSION     DAZFRG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.334"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            89..1419
                     /locus_tag="ACHKUC_004660"
                     /pseudo
     CDS             89..1419
                     /locus_tag="ACHKUC_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1447..2757
                     /gene="cyuA"
                     /locus_tag="ACHKUC_004661"
     CDS             1447..2757
                     /gene="cyuA"
                     /locus_tag="ACHKUC_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO6620762.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2818..2982)
                     /gene="yhaL"
                     /locus_tag="ACHKUC_004662"
     CDS             complement(2818..2982)
                     /gene="yhaL"
                     /locus_tag="ACHKUC_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO6620763.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(3005..3706)
                     /locus_tag="ACHKUC_004663"
     CDS             complement(3005..3706)
                     /locus_tag="ACHKUC_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO6620764.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKSESANFQLHGPRAYLQSIHGKFHALTHHKEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            3811..>4221
                     /gene="yhaJ"
                     /locus_tag="ACHKUC_004664"
     CDS             3811..>4221
                     /gene="yhaJ"
                     /locus_tag="ACHKUC_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6620765.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
BASE COUNT          925 a         1013 c         1214 g         1069 t
ORIGIN      
        1 aactcgcctg caaaaacgta gaccgcatat cgccttgatg cggctcggaa aaaaataata
       61 agccctacaa aatattgagc gagaaattat ggaaattgca tcgaataaag gcgtcattgc
      121 agacgcttcg accccggcgg gtcgtgctgg aatgagtgag agcgagtggc gagaagcgat
      181 caaattcgac agtactgaca ccggctgggt gattatgagt atcgggatgg cgattggcgc
      241 ggggattgtt tttctcccgg tgcaggtcgg tttgatggga ttgtgggtat ttttgctctc
      301 atcggtgatt ggttacccgg caatgtatct gtttcagcgg ttgtttatta atacgctggc
      361 agaatcaccg gagtgtaaag attacccgag cgtcatcagc ggttatttag gtaaaaactg
      421 gggcatcctg ttaggtgcac tctatttcgt aatgctggtg atttggatgt tcgtctattc
      481 caccgccatc accaacgata gtgcttccta cctgcatacc ttcggcgtga cggaagggtt
      541 gctgtcagac agtccctttt atggtctggt actgatttgc attctggtgg cgatctcctc
      601 acgcggcgag aaattgttat tcaaaatttc gaccggcatg gtgctgacca agctgctggt
      661 ggtcgcggca ctgggcgtgt cgatggtggg aatgtggcat ctgtataacg tcggttcgct
      721 accgccgctg gggctgctgg tgaaaaacgc cattattacg ctgccgttta ccctgacgtc
      781 gattctgttt atccagacgt taagcccgat ggtgatctct tatcgttcgc gggaaaaatc
      841 aattgaagtg gcgcggcata aagcattgcg ggcaatgaat atcgcgtttg gcattttgtt
      901 tgtcaccgtc tttttctacg ccgtgtcgtt cacgctggcg atgggacatg acgaagcggt
      961 aaaagcctac gagcagaata tttccgcgct ggcgattgcc gcacagttta ttagcggtga
     1021 cggcgcagcg tgggtgaaag tggtcagcgt cattctcaat atctttgctg taatgacggc
     1081 attctttggc gtctatttag gctttcgcga agcaacgcaa gggatcgtaa tgaacatcct
     1141 gcgtcgcaag atacctgccg agaagattaa cgaaaatctc gttcagcgcg gcatcatgat
     1201 tttcgccatt ttgctggcct ggagcgccat cgtactgaac gccccggtgt tgagcttcac
     1261 ctctatctgt agcccgattt tcggcatggt agggtgcctg atcccgggtg gctggtttac
     1321 aaagtaccgg cattgcacaa atacaaaggg atgtctctgt acctgattat cgtcactggt
     1381 ttgttgcttt gtgtttctcc gttcctggca ttttcttgat ttacctgaaa ttttaaggtt
     1441 tttaatatgt ttgattcgac tttaaatccg ttatggcagc gttacatcct cgccgttcag
     1501 gaggaagtaa aaccggcgct ggggtgtact gaaccgattt cactggcgct ggcggcggcg
     1561 gttgctgcgg ctgaactgga aggtccggtt gaacgtgtag aagcctgggt ttcgccaaat
     1621 ctgatgaaga acggtctggg tgtcaccgtt cctggtacag gaatggtggg gctgccaatt
     1681 gcggcggcgc tgggagcgtt aggtggaaat gctaacgccg ggctggaagt gctgaaagac
     1741 gcaactgcgc aggcaatttc cgatgccaag gcactgctgg cggcggggaa agtctcagtt
     1801 aagatccagg aaccttgcga tgaaatcctc ttctcacgcg ccaaagtctg gaacggtgag
     1861 aagtgggcgt gtgtcaccat cgtcggcggg cataccaaca ttgtgcatat tgagacgcac
     1921 aatggtgtgg tgtttaccca gcaggcgtgt gtgacagagg gcgagcaaga gtcgccgctg
     1981 acggtgcttt ccaggacgac gctggctgag atcctgaagt tcgtcaatga agtcccgttt
     2041 gcggcgatcc gctttattct cgattctgcg aagttaaatt gtgcgttatc gcaggaaggt
     2101 ttgagcggta actgggggct gcatattggt gcgacgctgg aaaaacagtg cgcgcgcggt
     2161 ttgctggcga aagatctctc ttcatccatt gtgattcgta ccagcgcggc atccgatgcg
     2221 cgtatgggcg gcgctacgct tccggcaatg agtaactccg gctcgggtaa ccaggggatc
     2281 accgcaacaa tgcccgtggt ggtggtagca gaacacttcg gagcggatga tgaacggctg
     2341 gcgcgtgcgc tgatgctttc gcatttgagc gcgatttaca tccataacca gttaccgcgt
     2401 ttgtctgcgc tgtgtgccgc aacgaccgca gcaatggggg ccgctgccgg gatggcatgg
     2461 ctggtggatg ggcgttatga aaccatctcg atggcgatca gcagtatgat cggcgatgtc
     2521 agcggcatga tttgcgatgg tgcatcgaac agctgcgcga tgaaggtttc gaccagtgct
     2581 tcggctgcgt ggaaagcggt gttaatggcg ctggatgata ccgccgtaac cggtaatgaa
     2641 gggattgtgg cgcatgatgt tgagcagtcg attgccaacc tgtgtgcgtt agcaagccat
     2701 tcgatgcagc aaacggatcg gcaaattatc gagattatgg cgagcaaggc cagataaacg
     2761 ccaggttttg ggtattgtgc cggataaggc gtttacgccg catccggcaa cttgtcttta
     2821 cgcggtagct tcatcttcgg ctaaacgcgt ttcagcatcc gcgacgatgg cttccagctc
     2881 gctcaacatc tcttcatagc caaaattttt ggcggtacga gcaggttctt tcgccaccac
     2941 gggcttcact ggctggcgct ttttggccga ttttttactc atcatcatct ccctgtaaaa
     3001 acaactagac aggcaaatct atcagcaaag cgcgcagtgg ggaatcggca accagcgtaa
     3061 tgttagcctc gtcacgaata aacgccccat caccgcaggt tagtgccgct ttctctttat
     3121 gatgcgtaag cgcatgaaat ttcccgtgaa tcgattgcaa atacgcgcgt ggcccatgca
     3181 actggaaatt cgcactttcg cttttgtcga gcacgatatg gtgcagccac acttgctggc
     3241 gcaattgcag gcttcccatc ccaccttctg gcgaggcgat taattgctgc ttgctcatat
     3301 taagcgccag cttttgaatc agcggattct ctcgctgcgg gcaggcatcc agccaaagct
     3361 gcattcgcgt taacggtttg tctttgctga gattgtgttc gctatagctg acacccggct
     3421 gggtagagag cagcaacacc tcaccggcgc tggcctgaac atgattgcct tcgctgtcgc
     3481 gatactcagc ttctccatcc agaatcacat ttaaaatatc gactttggga taggttcgcg
     3541 gctgaaaggc ggcacctggg gccagcactt cctggttaag cacgcgcagg gaggcatagc
     3601 ctaacaattt cgggtcgaag tagtgtccaa aggaaaaagt ataccgggcc tgcaaccatc
     3661 cgtagtctgc ttgtccacac tgcctggcag ttcgggtagt aatcataatt cttgacctct
     3721 ctttacttcc ttttatggta aggggctgga cgctacattg ttagccagat attctgcccg
     3781 gtatgttcaa atttcctgaa tgagaacgaa atggccaaag aaagggcatt aacgctggaa
     3841 gcactacggg ttatggatgc gatcgatcgc cggggcagtt ttgcggcggc ggcggatgag
     3901 ctgggacgcg tgccttccgc acttagctac accatgcaaa aactggaaga agagctggat
     3961 gtggtgctgt ttgaccgctc gggccatcgc accaaattca ccaatgtcgg gaggatgttg
     4021 ctggaacgag ggcgcgtttt gctggaagcc gcagataaac tgactaccga tgcggaagcc
     4081 ctcgcgcgcg gttgggaaac gcatctcacc attgtgaccg aggcgctggt accgacacct
     4141 gcctttttcc cgttaatcga caaactggcg gcaaaagcca atacccaact ggcaattatc
     4201 accgaagtgc tggcgggggc g
//
LOCUS       DAZFRG010000333         4184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFRG010000333 DAZFRG010000000
VERSION     DAZFRG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.335"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..405)
                     /locus_tag="ACHKUC_004665"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="ACHKUC_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(356..919)
                     /locus_tag="ACHKUC_004666"
     CDS             complement(356..919)
                     /locus_tag="ACHKUC_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6620766.1"
                     /translation="MNLIFSKYSVFFIFFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRISAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(919..3513)
                     /locus_tag="ACHKUC_004667"
     CDS             complement(919..3513)
                     /locus_tag="ACHKUC_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO6620767.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDANQLIPCLSLAD
                     LLSLGINKNALPEQALASSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYIFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKMLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVVWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYYNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELDNPV
                     SYATLECR"
     gene            complement(3550..>4184)
                     /locus_tag="ACHKUC_004668"
     CDS             complement(3550..>4184)
                     /locus_tag="ACHKUC_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6620768.1"
                     /translation="ASAGGFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDS
                     KTEKKSQDFVITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKN
                     TQKNTLQLALQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGI
                     KVQGSKLPNMFVPPKSDITVSSPVTLAGEITYQTINDFGATTERQKGIMQ"
BASE COUNT         1252 a          946 c          770 g         1216 t
ORIGIN      
        1 aaaaatcgtg agtgcaagcc cctctaccga gccttcttcc agcttatata aactggcatc
       61 ttctgaaact gtattaccgg taaactttat atacgcgttg ccaacatcac tacctcagcg
      121 ggttaacgtt atggaaaaat aaaccttttc agtaacgtct cctgcactcc ttaccgaact
      181 actgggtacg tctggcatat tagctgtaaa attttttgac tcatcagaaa taatgcaggt
      241 tttttcggta attaaccctt caatacgaac cactccggca tgagtgatat catcagcaaa
      301 agaacttgtt gatattataa acaaaaataa actaatgaat atcttcatat ctccctcact
      361 gataatcgat atcaaaatac aacagagcat ttgcttgccc ggcatgtaca ttttcctgag
      421 ttgatttata gcgaagttta aacgtcaaat tagcatcacc gttttcattt aacactaaat
      481 cacgaaaacc tgattcacca ttaatggcaa tcgccacatc attgttatcc aatatttcta
      541 ttcccagtcc agtcgcacta tctgcaccgg cggagatacg cagtaatgcg ctattaatag
      601 catctgcatc accagtaaac cgcacattgg cgctcacacc agaactataa acagtacttt
      661 ttgcagtcgg gcatccttgt acattaatcg taaacgtttg actcggagaa acatcaccta
      721 catttaaaaa ttcattagca tcataagtgc ccatttttac cgactgatct ttacttccgg
      781 tagtaatgtc acagcttttt gccaacaaaa tcccagtaaa tgtaacaaca actgaatcat
      841 tggcctgccc tgagagcgga aaagcaaaaa aagcgaaaaa aataaaaaat acagaatatt
      901 tagagaatat taaattcatt agcggcactc cagagttgca tagctaacag gattatccag
      961 ttctgtttca gggagcttgt aattagcctg acatttttgc tgaacaccgt ctccccactg
     1021 gatattaata acaccttcta aaggcaatcc tgagagataa agttgtccat tatcgccgac
     1081 aatcccggta atttgactac tgtttttaat ctgagcgctg gctgtagcac cgaatgggac
     1141 tgatttatta cgataacgaa ctgtgaacaa accacgaata ccgatatgag cagcaaactc
     1201 tgcacgaact aacgcacctt ttcccggaat aacttcagtg gaagtgatat ccagttcagt
     1261 atgcatattc attgacatag gatctaatga aatattatta cgtctatatt ctgttgcata
     1321 aggaacaact gcatatccac gccagtcagt ggatattcct gtcccccgct gaacatccac
     1381 attattcgct ccgggcgctt ttatcaaaac attagtttca cctaatggtt gactcaacgt
     1441 aagaccattt tcatgtacaa caagagcacc actgatgcca tagtttattt gttgattacc
     1501 ttcattatag taactgtagg cggaattcaa ggaaccatat gccccacgat aattaacacc
     1561 aacagaacca ctactgctat tatcctgatt agcaaaacca tgttgtatgt tataactgag
     1621 attatgctgc tccaacagag aaccagatac ccccgtatat tggtttacct gtccgtggtt
     1681 atcctgacta taaccatacg ttgcagtcat ggcattcgat gatgagcgcc catcattcgt
     1741 ggaagataac caattactta taggtaatga gacatttagc gagacaattt tatccgcatt
     1801 tgtccctggt gatttactgt aattccacac aacatttaga tagataccct taatactggt
     1861 gttataacca acttggtata aagaatcttt tttatcagta ttccagtatg tttgctgact
     1921 agccgataaa tacatagacc cgtagttacc gaaaggttgc gatacactag cctgaaattt
     1981 tccacgtttg ctataacgca agttataaaa atcattccag ttataaacgt aatctttatc
     2041 atctaacatt tttggatcaa caacagtccc tgacatttgt tggtatgtcg tatcacttaa
     2101 agtgtaaaag ccctgggttg aataacgata gccaataata tggaatgctg ttcctgtctg
     2161 aactaacgat ttggaataaa gaaaacgata agattgccct tgatgtttgc tcccatccac
     2221 aagcgaactg ttagcctgag taacatcaaa cgatgttgca ccaaatactc ccagattcag
     2281 gccaaggccg agcgccaacg cccggtaatc ctcagagaat tgtgccccac cataagccgt
     2341 aatatcccag ggtaaacccc aaattaattc cccctgaaat atataaggac ttttctggtt
     2401 atagctattg cttcgatatt ggccaaccgt agtagaatat tttgaatgac cttctcgttg
     2461 caggatgggg acagcggcat aaggtacggt atagacagtt tctgaattat cagactcttt
     2521 gatggtaact tccagatctc ccgcagatga tgtcgggtta agatcgctaa tctcaaacgg
     2581 tcccggcggc atataggttt gataaatgac ataaccattc tgtttgattg ttacctgagc
     2641 gcgacttttc gcaattccgc gcaccgtggg agcgaaacca ctttgactat cgggcaacat
     2701 attttcgtca gattccagtt ttatgccacg aatactaaca ctgtcgaaaa catctgatga
     2761 tgaccatgta tctcctagcg ttaattcact tctgaaagga ataataacgc gctgcaacgt
     2821 actactgata tgctccagtt tacctgctga attactccca cgactccagg ttgaataatc
     2881 gcgaaaacgc catgcaccaa tattgatccc agcgcgaaga tttaaataat agttatttga
     2941 agagtaatca ccacgatcgt tattaccaga aaagtcataa ttcagcaaaa aagctgtaat
     3001 accgttatcc cactgttctg gtggaatata tccgcgggcg tcacgtttta ttatcgcctg
     3061 cggaaaggtc agtttaagtc tctgtgcatc cagctccggc atgtaatgca catcaggaaa
     3121 ccagatacgc aaatcaaggc aactattttc agatgaagcc aaagcctgct ctggcagcgc
     3181 atttttgtta attccaaggc ttaataagtc agcaagtgaa aggcagggaa tcagttgatt
     3241 cgcatcgtct gctttaaaag ttacagtacg agagagtaaa ggggaatcat tgacccaaac
     3301 ttcgacttca tagctaccag gaagataagt catccctttc tcgaagcggg ataagtcagc
     3361 aacagcttca gtatcacccg acagaaaagc agggttaaag cgcattcccg cgattgaaca
     3421 tgcaggcata ataaccagaa gaagcggcac aacccgtttc atctgctttt gtgccgccat
     3481 ccaatattcg catcttttga gctttaggtt catatccctg acctgtgttg catctgaact
     3541 attccgttgt tactgcatta ttcctttctg ccgctccgtc gtagcgccaa agtcattaat
     3601 tgtttgataa gtaatttcac cagccaacgt cactggcgac gagaccgtaa tatcactttt
     3661 cggtggtaca aacatattgg gcaatttact cccttgtact ttaattcctg tcagtgtcag
     3721 gtaataaggc gaaggattga taacttttag ttgttttccc tcatacttaa accgcaacat
     3781 atttcgtgct tttcctggtt gcacaggtag attttccggg cgataaaata gcttgatttt
     3841 gttttgtaat gccaactgta aggtattctt ctgtgtattt ttctcatctg ttggcggaat
     3901 tgctttaaca ttcatccaat acaaagtttc acgatcggtc ggcaaattag tcttaccaag
     3961 aaacatgata cgtaatgaag cttctttacg cgttggcaac ataaagaggg gaggagtgat
     4021 aacaaaatcc tggctctttt tttccgtttt actgtcatca acccatgatt gaattaaata
     4081 actggtgttg tcaccgctat tttgcacact gagtgttatt tgcttacttt ctgccggata
     4141 aatcaatcgc gttacgccaa ggccaaaccc gcccgcggac gcat
//
LOCUS       DAZFRG010000334         4180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFRG010000334 DAZFRG010000000
VERSION     DAZFRG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.336"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(141..2054)
                     /gene="mtlA"
                     /locus_tag="ACHKUC_004669"
     CDS             complement(141..2054)
                     /gene="mtlA"
                     /locus_tag="ACHKUC_004669"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO6620769.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWIDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            2591..2953
                     /locus_tag="ACHKUC_004670"
     CDS             2591..2953
                     /locus_tag="ACHKUC_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6620770.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2956..4092
                     /locus_tag="ACHKUC_004671"
     CDS             2956..4092
                     /locus_tag="ACHKUC_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6620771.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1028 a         1076 c         1079 g          997 t
ORIGIN      
        1 attgtgtacc gactgccgga tgcggcgtaa acaccttatc cgaccgacaa aacaaattgt
       61 gctttatgtt gtaggcctga taagcgaagc gcatcaggca attagcccca ccttcttcat
      121 gcggagaggg tgggaatgga ttacttacga cctgccagca gttccagcac ttcatccacg
      181 ctggtggtgt gtgccagacg ctcgatgaca gactcatcat ccagtgcatt ggtcaggctg
      241 gtgataacct gaatgtgctc gttgttacgg gcagcaatac caatcaccag acgggcaatg
      301 tcatcttctt cttcaccgaa gcgcacgcct tccggatact ggcagaacac cacgcccgtt
      361 ttcagtacgc gatctttcgc ttcaaccgta ccgtgtggca ccgcgataga ctcacccaga
      421 taagtcgggg tcagtttttc acgatccagc atcgcctgaa cgtattccgg ctcaacgtaa
      481 ccgcctttca ctagctgctc gccagcaaaa cgaatcgctt cttctttggt ggccgctttg
      541 cgaccgagga agatgttctc cgcgcccagt ttgaacagat tagcactgga atcgtcaaag
      601 ctgtctttca gactgtcttt cactttcact tcgttttcag tatggcgctg ggcagcaacc
      661 agacgttcgg tcaggctggt gtacaggccg ctgtcgagga agttggtcag cgaaatatgc
      721 tgtgcctgcg gaacctggcg catagcgcgt tcggtcaggt cacggtgagt gatgaccagg
      781 tccacatctg gcggcaggtt gttgatcgcg ctgttagtga ccgaaatctg cgacagacct
      841 gcatcctgaa ttttcttacg cagcacacct gcgcccatcg cactggaacc cataccggcg
      901 tcacaggcaa cgatgatttt acgtacgtgg ctcaggtcgt tagtcacatc gcccgcagac
      961 agcggagacg cacctttaga ctcagctttc atgtcctgca tacgacgagt tgctgcttca
     1021 atatcatctt cttctttcac tttgctggtt ttcagcagaa tggcagagac aacgaaggag
     1081 acagccatcg ccgcacacac acccgcgatg ttagcgaagt atgcaccttt tggtgtcatc
     1141 gccagtacag caaggataga acccggagat gccggagaaa ccagcccacc gcccaggata
     1201 gtcagcgtga acacgccagt cataccaccg aggataacgg ccagaatcag acgcggattc
     1261 atcagcacat acgggaagta gatttcgtgg atacccccca ggaagtggat gattgccgca
     1321 ccgcccgcag actgtttagc gctaccacga ccaaagaaca tgtacgccag cagcacgccc
     1381 atacctggac ctgggttagc ttcaatcagg aagaagatgg atttacccag ttcatgggac
     1441 tgctgaatac ccagcggcga gaaaataccg tggttaatag cgttgttgag gaaaaggatt
     1501 ttcgccggtt caacaaagat agacgccagc ggcagcatgt catggacaac catgaagtta
     1561 acgcccgcag ccagcatttt agacagggct tcaacaaccg ggccaatgcc gagaaatgcc
     1621 agaatagcga ggatcatccc gatgatgcct gcggagaagt tattcaccag catttcaaaa
     1681 ccggatttga tcttaccgtc tatccagcgg tcgaagtgct taatgcacca gccgcccagc
     1741 ggacctgcaa tcatagagcc gaggaacatc ggcatatctg cgccgacgat aacgcccatg
     1801 gtggtgatgg caccgactac gccgccacgt tcgccgccta ccagcttacc accggtataa
     1861 ccgatcagca gcggcaggag ataagtgatc atcggcccga ccagcttcgc cagcgtttcg
     1921 ttcggtaacc accctgttgg aataaataac gcggtgatga taccccacgc gataaacgcg
     1981 ccgatatttg gcatcaccat gttgctgagg aaacgaccaa agctttgcac tttgatctta
     2041 atatcggatg acataaaaac accccttctt atgtttgctg tcgcgcaggc tggaagcccg
     2101 aggttttttg ttaatgtggc ggcagaggta gccggacctt gtagatactg cgaaatctgg
     2161 cactgaatcg gttaactgtc cagtcgacgg tcttttgtgt gatatcagtc acataaatat
     2221 agggggtaga gaggtaattg acgtgatgct catcacaaaa tgactgtgta aaaaagcaac
     2281 aagacggcga ttttcgctaa aaatggcatt taaatgtgag taatgtcaca tttttgtttt
     2341 gttagtttgt tgaatctttg tgatctgaat cacaagatat tttcttacgg cgttcacctg
     2401 atgtgatctg cagcatgtta tgccttcgtc ataaaatcgt tatgtaacta agcaactcat
     2461 cacctctaaa aattattctt tggtgttatg gcgagaatcg cgaacccgaa ttattgtcaa
     2521 aatatcagcc tgactccctt ctggctgcca gccaaaaaac aacaccgata aacgtgaatc
     2581 cggggcagat atgtttctaa actatttcgc gttgggagtg ctgatcttcg tctttctggt
     2641 gattttttat ggaatcatcg cgatacatga catcccttat ctgattgcca aaaagcgcaa
     2701 ccatccccat gccgacgcta ttcatacggc gggctgggtg agcctgttta ctctgcatgt
     2761 tatctggccg tttctgtgga tctgggcgac gctctatcaa ccggagcgtg gctggggtat
     2821 gcagtcacat gttgcgtcgc aggagaaagc gactgacccg gaaatcgctg cactttctga
     2881 ccgaatttcc cggctggagc accaactcgc cgccgagaaa aagactgact attccacgtt
     2941 cccggagatc taaatatgga tctattgatt gttttaactt acgtggcgct ggcgtgggcg
     3001 gtctttaaaa tcttccgcat tccggtgaat cagtggacgc tggcgacggc ggcgctggga
     3061 ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact acaaccaccc ttacactttt
     3121 accgcgcaaa aggcagtgat agcgatcccc atcacgccac aggtgacggg aattgttact
     3181 gaagtcaccg acaaaaataa tcagcttatt caaaagggcg aggtgctttt taagctcgac
     3241 ccggttcgtt accaggcgcg agttgacaga cttcaggctg acctgatgac ggcgacgcat
     3301 aatataaaga cgctgcgcgc gcagctcact gaagcgcagg ccaacaccac ccaggtttca
     3361 gcggagcgcg accgtctgtt taaaaattat caacgttacc tgaaaggcag ccaggcggcg
     3421 gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc aaaatttcct cgcgcaggac
     3481 gcgctggtga aaggctcggt ggcggagcag gcgcagatcc agagccagct cgacagtatg
     3541 gttaacggcg agcagtcgca gattgtgagc ttaagagcgc aacttactga agcaaaatat
     3601 aaccttgagc agactgtcat tcgcgcgccg agcaatggct acgttactca ggtactgatc
     3661 cgcccaggta catacgcagc tgccttgccg ttgcgtccgg tgatggtttt catccccgag
     3721 caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc tgttacgtct gaaacccggc
     3781 gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg tgttccacgg caaactgacc
     3841 agtattttac ctgtcgtacc aggcggttcg tatcaggcgc aaggggtatt gcaatcatta
     3901 acggtcgtgc ccggcacgga cggtgtgctg ggaaccattg aactggaccc caacgatgat
     3961 atcgatgctt tacccgacgg catctacgcc caggtggcag tttactccga ccatttcagc
     4021 catgtttcgg tgatgcgaaa agtgctgctg agaatgacca gttggatgca ttatttgtat
     4081 ttggatcatt gagggggaag gggcttggag gtacaggcgc tattggcaca gttctggctt
     4141 actggctatt gccagaactg gtagctaatc tcatgtcatt
//
LOCUS       DAZFRG010000335         4174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFRG010000335 DAZFRG010000000
VERSION     DAZFRG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.337"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            107..901
                     /gene="hyxR"
                     /locus_tag="ACHKUC_004672"
     CDS             107..901
                     /gene="hyxR"
                     /locus_tag="ACHKUC_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO6620772.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1055..1816
                     /gene="hyxA"
                     /locus_tag="ACHKUC_004673"
     CDS             1055..1816
                     /gene="hyxA"
                     /locus_tag="ACHKUC_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO6620773.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYQDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHNWEYSAQFLKTGIRQIWLTEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1858..4155
                     /gene="hyxB"
                     /locus_tag="ACHKUC_004674"
     CDS             1858..4155
                     /gene="hyxB"
                     /locus_tag="ACHKUC_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO6620774.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTDSVTLNKDKTT
                     LDQDVVINNTADKQITAVTINAANEDLNVTFAGHDITAESTADKKFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVISSTGDVVVNGGNFVAKNEKGSATGISLEATTGNN
                     LTLNGTTINAQGNKSYSNGSTAIFAQKGNLLQGFDGDATDNITLADSNIINGGIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGAGSATQNLNLSVADSTIYSD
                     VLALSESENSASTTTNVNMNVARSYWEGNAYTFNSGDKAGSDLDINLSDSSVWKGKVS
                     GAGDASVSLQNGSVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWKDGNGLFIQPYAKLSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
BASE COUNT         1157 a          898 c         1049 g         1070 t
ORIGIN      
        1 aattagattt atattccaag aaaattaaat attcaggcta ttaaaccaaa cctgacattt
       61 tgaatcaaaa tgtcaggttt tgtagcaatg gcgtggacga gtctgaatga agaagcctct
      121 ggttattatc agtgcctgtc agttcactag gttggcactg gagtctctca tcccaacgga
      181 caggtatatc gtgcgggtgt acagcaacgt cactacagag gttgaattcg ttttaaagac
      241 ctcctgtggc tacctgttag ctgatatccc ttcgcttcct tcaggggagt tggttcttct
      301 ggcttgtctc gcccgtttgc ggagtgatgc cgggcaatgg caggtctgcc tgaccggtga
      361 ctccgactgg ttcgataatg ctcctgctgc aagtctgtat aacggcttcc cacgcatcgg
      421 caccatgctg acagcgagtc gttatcagaa tcaaattatc cgctggttac tgcgtccttt
      481 gcccggcgat atgagcgatt tctggctcct gactgcacgg gagttgagcg tgcttaagat
      541 tctgatgcag ggcatgagtt tcagtgagat tgcccgttat gaacaacgca gttcaaagac
      601 cctgcatgcg attgccacac gggcattaat gaaacttgga ctgggaactc tgagcgattt
      661 caggctgctc tatacgggat gcggtgacag cagagtgaaa cggaatatac ggctacatgc
      721 aagatatgtc ggacaacatt ccgctcatcg actcactcag tatgtgcaga acatggtatc
      781 cggaattctt tatccagaga aagttgccgt tggtgaatca ataaattctg acgccatatc
      841 cggaaaagta ctttccggac aaagaaaaaa tatgtctaaa aaaagaccag gattgttatg
      901 acacagcagc gttgttacta ccccccactg cggcgcatac ggactcgcta cagtgtagga
      961 gccaaaatat ttctactgct ctgggctgca ggattgggag gcacgctgta cagcctgtac
     1021 agcgtgctgg cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt tctgtccctc
     1081 aggaattgta ccagcgcagt tcgcagctct atcaaccggt tctgtcggca atgttacctg
     1141 catcagaaca gaagaggagt tacgcaataa attgcggcat aggccacaga gtgtcgtgat
     1201 atcggcaggg cgacctgccg aatgcgctga aatgtggttc cgattttatc aggatcactc
     1261 attcgtggtg gtgttgtgcg tcgcgccctt ttttctgcca ccggatgtga gtatttctgg
     1321 cgttctcaaa aacctgaggt tactgaaacc aggaatgtca gtagagcgtg tcatcagtat
     1381 tgcaaatgcc tctggtgggt tttcagggct gaaacatgca gaaattttgc cagttatgga
     1441 cagttactct gtctttatga aagaggtgaa taaccgcaca aagacaatcg tcatgtcaga
     1501 acgcttcccg gaaaagcaaa agaaggtgtt gtcgttactt cttgccggac acaactggga
     1561 atacagtgcg caatttctca aaacggggat aagacagata tggcttaccg agcagtcgct
     1621 aaaaaagcgt tggggaatac cggacagcat gtcattgcgg gaagccctgc tgctgagcag
     1681 caataaattt cacggtgatg gaaacgcatt ggaaacaacg aatgccatga cattgcgtga
     1741 aaacggaaat accaacaggt attccgtggt ggtaaatgca gggacgcagg ctttatctct
     1801 gcataaatat aaataagtgg aaaattgaaa ttagttacca gaatggagaa tttcttcatg
     1861 aaaaacagta aagcatttta ccgcagcgca ttagcaacag ctattgttat ggctctttct
     1921 gcaccagcat tcgctgctga taacgcggta tcaactgatt cggttacgct gaataaagat
     1981 aaaacgactc tggatcaaga tgttgttatt aacaatacag cagacaaaca gattacagcc
     2041 gtaacaatta atgcggcaaa tgaagacctg aatgttactt ttgctggtca tgatattact
     2101 gctgaatcaa cggcagacaa aaaattcgtt gaaggtgtaa aagttagtgg taacaaaaat
     2161 gttgtgatta atgctacaga ctccaccatc acagctcaag gtgaaggcac ctatgtccgg
     2221 actgcaatgg tcatttcttc aactggcgat gttgttgtta atggcggtaa tttcgttgca
     2281 aaaaatgaaa aaggtagtgc gacagggata tctctggaag cgaccacggg aaataattta
     2341 acgctcaatg gtacaaccat aaatgctcaa ggtaataaga gttacagcaa cggctctacg
     2401 gcaatttttg ctcaaaaggg taatttgttg cagggttttg acggtgatgc aaccgacaac
     2461 atcacccttg ctgactcaaa tattattaat ggcgggattg aaacaatagt tactgccggg
     2521 aataagacgg gaattcatac agtcaacctg aatattaagg atggctcagt aattggggcg
     2581 gctaataata aacaaacaat ttatgcctct gcttcggcac aaggcgcagg ttcagcaacg
     2641 caaaatttaa atttgtctgt tgctgattca accatctact ctgatgtcct ggccctttct
     2701 gaaagcgaga attcagccag taccacaaca aatgtaaata tgaacgttgc ccgctcttac
     2761 tgggaaggta atgcttatac cttcaatagc ggcgataaag cgggtagtga tctggatata
     2821 aatctttccg atagttcagt ctggaaaggc aaagtttcag gggcaggaga tgccagtgta
     2881 tctctgcaaa acgggtctgt ctggaatgtt acgggttcct caactgttga tgctctggca
     2941 gtaaaagaca gtacggttaa tatcacgaag gctacagtca atactggcac gtttgcttct
     3001 caaaacggca ctctgattgt tgatgcctct tctgaaaaca ctctggatat cagcggtaaa
     3061 gcgagcggtg acttgcgtgt ttacagtgcg ggttcattgg atcttatcaa tgaacaaacg
     3121 gcatttattt ctaccggcaa agacagcact ctaaaagcca caggcacaac ggaaggtggt
     3181 ctgtatcaat atgacctgac acagggagct gatggtaact tttatttcgt aaaaaacacg
     3241 cataaagcat ccaacgccag ctccgtgatt caggcaatgg cagctgctcc ggctaacgtc
     3301 gctaatctgc aggctgacac gctctccgcc cgtcaggatg ctgtccgtct gagcgaaaat
     3361 gacaagggtg gcgtatggat tcagtacttt ggcggtaaac agaaacatac caccgcggga
     3421 aatgcatcct atgacctgga tgtaaatggt gtaatgctgg gtggtgatac ccgcttcatg
     3481 actgaagatg gtagctggct ggccggtgtg gcgatgtctt ctgcgaaagg tgacatgact
     3541 accatgcaga gcaaaggtga cactgaaggt tacagcttcc acgcttacct gagccgccag
     3601 tataacaacg gtatcttcat tgatactgct gcacagtttg gtcactacag caacacggca
     3661 gatgttcgcc tgatgaatgg tggcggtacc atcaaagctg actttaacac caatggtttt
     3721 ggtgcgatgg ttaaaggcgg ttacacatgg aaagacggta atggcctgtt tattcagcca
     3781 tatgccaaac tgtctgctct gactctggaa ggtgtggatt atcaactcaa cggcgtggac
     3841 gttcattctg acagctataa ctctgtgctg ggtgaggccg gtacgcgcgt gggttatgac
     3901 ttcgctgtgg gcaacgcgac cgttaaacct tatctgaatc tggccgcact gaacgaattc
     3961 tctgatggca acaaagtccg tctgggtgat gagtctgtca atgccagcat tgacggtgca
     4021 gcattccgcg tgggtgcagg tgtacaggct gatatcacca aaaacatggg agcatatgca
     4081 agccttgact acaccaaagg tgacgacatt gagaacccgc tacagggtgt agttggtatc
     4141 aatgtgacct ggtaagaata aagcagtcgg ttcc
//
LOCUS       DAZFRG010000336         4141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFRG010000336 DAZFRG010000000
VERSION     DAZFRG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.338"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..134)
                     /locus_tag="ACHKUC_004675"
     CDS             complement(<1..134)
                     /locus_tag="ACHKUC_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6620775.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="ACHKUC_004676"
     tRNA            complement(249..324)
                     /locus_tag="ACHKUC_004676"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="ACHKUC_004677"
     tRNA            complement(331..405)
                     /locus_tag="ACHKUC_004677"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="ACHKUC_004678"
     tRNA            complement(522..606)
                     /locus_tag="ACHKUC_004678"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="ACHKUC_004679"
     tRNA            complement(615..690)
                     /locus_tag="ACHKUC_004679"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="ACHKUC_004680"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="ACHKUC_004680"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO6620776.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="ACHKUC_004681"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="ACHKUC_004681"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO6620777.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="ACHKUC_004682"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="ACHKUC_004682"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO6620778.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNTGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1085 a         1025 c          920 g         1111 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgtgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg gagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctctgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtaccaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggtagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaactgt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttaca gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 tattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgcgagcgtg ccagtttaat cctgacgaaa agtgctaatc atgcagtaga
     1981 agaggtcaga ctacgcaaat aatttaaagg ggagttaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagcac cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccaactttt cccagcgcga aagataaggt
     2221 gtcaatcctt cttgctcgaa gagttccagc gccgcgcgta attcacgtat cagcatggcc
     2281 gcaagagtat tacgatcgag attgatcccg gcttcctgca gcgtgatcca cccttgatta
     2341 acgacactct cttcaacacg acgcattgcc atgttgatcc ctgcaccaat gactatttgc
     2401 gccgcatcgc cggttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttaacacga actttatctg cacccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccctgttcc
     2581 agacgccaga acatcgacaa atataagttc gcgccaaaag gcgaaaacca tttccgacca
     2641 cgacgaccac ggccagcatg ctggtattct gcaacacagg catcgccaga tttaagctct
     2701 ccgatgcggt ccaggagata ctgattcgtg gagtcaataa ctggcagcac ggctacactg
     2761 ccgtcatcca gctgactcaa tatcttttcg gcattaagta actggattgg ctcgggcaag
     2821 ctgtatcctt tacccggaac agtaaataca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcgccat tcgctaacag ggcgatcaat ttcagtggca cggtgttatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatgatg cgccagctgt acaacatctt
     3121 cgctttttgc atcgtgctca tttatgagaa ccagcgcctg ttgacggtgt accgcagccc
     3181 caccaatttg cgtcccttta agctggcact gatcaataag ccaacctgca gccagtttta
     3241 ctgaaccatc cacctggggg taatgtgggg ctgttggaaa ttgtgccaac aacgcgttag
     3301 cagtttcggc agaaacaaca ggatttttga agaaactacc cgtattaccg ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatc aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg tacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcctccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgat gacaggaaga gtttatagac acgcaaaaag gcccagtctt ccgactgggc
     4141 c
//
LOCUS       DAZFRG010000337         4138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFRG010000337 DAZFRG010000000
VERSION     DAZFRG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.339"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..306
                     /locus_tag="ACHKUC_004683"
                     /pseudo
     CDS             1..306
                     /locus_tag="ACHKUC_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            547..1512
                     /gene="lipA"
                     /locus_tag="ACHKUC_004684"
     CDS             547..1512
                     /gene="lipA"
                     /locus_tag="ACHKUC_004684"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO6620779.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1612..1815)
                     /gene="tatE"
                     /locus_tag="ACHKUC_004685"
     CDS             complement(1612..1815)
                     /gene="tatE"
                     /locus_tag="ACHKUC_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO6620780.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(1944..2732)
                     /locus_tag="ACHKUC_004686"
     CDS             complement(1944..2732)
                     /locus_tag="ACHKUC_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO6620781.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICGSLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWGRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2828..3211
                     /gene="crcB"
                     /locus_tag="ACHKUC_004687"
     CDS             2828..3211
                     /gene="crcB"
                     /locus_tag="ACHKUC_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO6620782.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3265..3474)
                     /gene="cspE"
                     /locus_tag="ACHKUC_004688"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3265..3474)
                     /gene="cspE"
                     /locus_tag="ACHKUC_004688"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6620783.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3649..>4138)
                     /gene="pagP"
                     /locus_tag="ACHKUC_004689"
     CDS             complement(3649..>4138)
                     /gene="pagP"
                     /locus_tag="ACHKUC_004689"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO6620784.1"
                     /translation="ANADEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKT
                     DRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENF
                     HLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNNGNVYFAWMRF
                     QF"
BASE COUNT         1074 a         1067 c          940 g         1057 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaaatcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttac ccttctgact attttgaagt tgcatatgtc cataaaggaa
      121 tcgttaatgc agcgaaagtg cttaatctgc cccccctcgg tcggagccct ggtccttcct
      181 gtcatctatc gggcgattaa aactcactat ccacagcttt tactgcgcaa cccgcccatc
      241 agcggcgcgg aaaaccaact cagtcagttt caaaccgatc tcatcaccga taacatgttt
      301 tgcacaggac catagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat
      361 cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct
      421 ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca aaaatagttg
      481 ataattacaa caatctattg aattgaaacg cttttcttcg taattcgcaa ctggaacacg
      541 cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag
      601 atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg
      661 gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca
      721 atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa
      781 tgctttaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac ccgccgttgc
      841 ccgttctgtg acgttgccca cggtcgcccg gtagctcctg atgccaatga accagtgaaa
      901 ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt
      961 gatgacctgc gcgatggcgg tgcccagcac tttgcggatt gcattactgc cattcgggaa
     1021 aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgtggtcg tatggatcgt
     1081 gctctggata ttctgactgc aacgccacca gatgtgttca accataacct ggaaaacgta
     1141 ccgcgtattt accgtcaggt acggcctggt gcagattaca actggtcgct taagctgctg
     1201 gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggactg
     1261 ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg
     1321 atgctaacgc tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac
     1381 gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat
     1441 gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc gaaagggatg
     1501 gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga
     1561 agcgggtttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac gtcactcttt
     1621 atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg cagcatcgtc
     1681 atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc ccagcgtacg
     1741 taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca gtttggtaat
     1801 actaatctca cccatagata ccttcttgac atataaccgg caacagaacg cgccttaaaa
     1861 accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt aacaaggtga
     1921 atcaagcacc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg acggttgttt
     1981 aagacgggca gttgcgcgcg tacctgacgc acacgttcgg gcgtcacttc cgccataatg
     2041 agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat aatccggctt
     2101 tgcccgatat ttttgttccc gcactccccc gccgccacca tataacaggt ggtatccagc
     2161 gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccacg accccaggcg
     2221 gcaggaagta ccaggatttc agctccctgt aatgcctgtg ccagcgccag ctctggaaag
     2281 cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag taacggagct
     2341 atttcattac cagcatcaac atggcgtgat tcctgaatgg caaatgcatc atagagatgc
     2401 agtttggcat aacgggcgac gatgtttcct gcctgtagcg ccaccagcat attccatgcg
     2461 cgccccggcg ttgaaggaac atgaatcgtc agaattgtcg tcatcatgtt acgtttactt
     2521 tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt aaccgataga
     2581 tctgcatcat gatcatcacg cgccagtaat gcttccggca ggacaaacag cgaaacgtcg
     2641 ttttccgccg cctgcgccat caacgagcca caaatctcag cgttcttttc ccacacagat
     2701 gtaacagcaa actgtcctgc ggcaaccagc attgccacct cctcctgtca gagtcgtaaa
     2761 attcgccaac ggcgattatt cggcgttaca cttatcactc atacaaatca aatagcagga
     2821 ttttgcagtg ttacaacttc ttttagcagt ttttataggc ggtggtacgg gaagcgtggc
     2881 gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct
     2941 ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat
     3001 gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac
     3061 aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg
     3121 ggcgttactg aacgttttcg tcaatctttt ggggtctttt gccatgaccg cactggcttt
     3181 ctggctgttt tcggcctcaa ccgcacacta aaaaccaaaa aaaacccgct gattaagcgg
     3241 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
     3301 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
     3361 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
     3421 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
     3481 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt
     3541 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgtta
     3601 attattttac tggttgattt taaaggattt actaaaactt catttgtctc aaaactgaaa
     3661 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
     3721 aaacgtcgcg gggccatacc ccacagaggc caatggcagt agaaccggaa gagggatgta
     3781 attccagtta tcgcgcgccg ttacgccagc ggtaaatccc aggcctaaat gaaaattttc
     3841 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
     3901 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
     3961 cgactgacca aaaccgccgc cccacggtcg ctcgttatag cgatcggttt tttctttgtc
     4021 gtaagcaaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttctggctg
     4081 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc cactcatcag cgttagca
//
LOCUS       DAZFRG010000338         3963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFRG010000338 DAZFRG010000000
VERSION     DAZFRG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.340"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1162
                     /locus_tag="ACHKUC_004690"
     CDS             <1..1162
                     /locus_tag="ACHKUC_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6620785.1"
                     /translation="STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNG
                     VFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFN
                     SLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFL
                     AYAGFGMMANAADKVKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYAD
                     TAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKP
                     LWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASL
                     PILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            1193..1290
                     /locus_tag="ACHKUC_004691"
                     /pseudo
     CDS             1193..1290
                     /locus_tag="ACHKUC_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1287..1694
                     /gene="cueR"
                     /locus_tag="ACHKUC_004692"
     CDS             1287..1694
                     /gene="cueR"
                     /locus_tag="ACHKUC_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO6620786.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1698..1901)
                     /locus_tag="ACHKUC_004693"
     CDS             complement(1698..1901)
                     /locus_tag="ACHKUC_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620787.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTEIN"
     gene            complement(1898..2320)
                     /locus_tag="ACHKUC_004694"
     CDS             complement(1898..2320)
                     /locus_tag="ACHKUC_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620788.1"
                     /translation="MMNSSIKSFSLLAVILLTGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(2406..3455)
                     /locus_tag="ACHKUC_004695"
     CDS             complement(2406..3455)
                     /locus_tag="ACHKUC_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6620789.1"
                     /translation="MKADDAQHAIMVAQTDIDTNKASIADTRSDVSAVQSDVTNIKGD
                     VAHAQSTADHANANANTALMNGVKLSSAVTENKNNIEQNRSDIADQQKQLDETQKTVA
                     ATGDVQTAARYQSMIDARQTAAANAQQEQADTQQQQITDQQKQIDATQKTVSALGDAQ
                     TSAHYQEMVNARLSAQNDANARTAAEQKQKIDTLATNQETQQHIDSVQYGEQIQRLAQ
                     DSTQTHEQIDSLTQDATQTHQQLSNTQKRVADNSQQINTLNNHFSSLKNEVEDNRKEA
                     NAGTASAIAIASQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISAD
                     TQSDFGAGVGVGYSF"
BASE COUNT          828 a          937 c         1038 g         1160 t
ORIGIN      
        1 ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg atgttttccg gttatgccta
       61 tgcgcgtctg ggggcgagct atcccagcaa tggcggtatt atcgacttct ttcgtcgcgg
      121 attaggcaac ggcgtttttt cgctggcgct ctcgttattg tacctgttga cgctggcggt
      181 gagtatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt tgcatgaagg
      241 cagccaggag gagcacctta ttttgctcta cgcattgggg atcattgcgg tgatgacgct
      301 tttcaactcc ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg tcggcattaa
      361 aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac cggcgcatat
      421 ttccgtttct gcgcccccca gttccggggc gttcttctcc tgtatcggga taaccttcct
      481 tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag atccggaggt
      541 cataatgcca cgggcgtttc tggtggcgat tggcgttacc acgttgcttt atatctcgct
      601 ggcgctggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg ccgataccgc
      661 cgtagcgcag gctgcttctc cgctcctcgg gcatgtgggg tatgtgatcg tcgtcatcgg
      721 cgctttactg gcgacggctt cagccatcaa cgcaaacctg ttcgccgtgt ttaacatcat
      781 ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg aataaacctc tgtggcggca
      841 gagtacctgg ggcaacatca tcgtcgtggt gttgattatg ctgatgacgg cggcgctgaa
      901 tttaggctca ctcgccagcg ttgccagcgc cacctttttg atctgctatc tggcggtgtt
      961 tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc ttatcgttgg
     1021 tacgttggtt atgttgttgg tgatcgttgg ctttatctac agtctctggt cccagggcag
     1081 ccgtgcgctg atatggatta ttggcgcact cttactcagc cttattgtgg caatggtcat
     1141 gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt acttcctgta ttattgtggt
     1201 ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat atttgttgaa accctttaac
     1261 aaagcacagg aggcgttgcg cgaacgatga acattagcga tgtagcaaaa attaccggcc
     1321 tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca
     1381 gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa cgaactgacc ttactgcgcc
     1441 aggcacgcca ggtgggcttt aacctggagg agagcggcga actggtgaat ctgtttaacg
     1501 atccgcagcg tcatagtgcc gacgtcaaac gtcgtacgct ggagaaggtg gcggagatcg
     1561 aacggcatat cgaagaactg caatccatgc gcaaccagct actggcgctg gcgaatgcct
     1621 gccctggcga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc
     1681 atcgggcggg gtgaaaatca gttaatctcc gtttgcaccg aaagaatatg ctgcaaactt
     1741 tctaccataa tgcgccccga ctgaaataag ccttcctgga tttcgttgtt aacattcaca
     1801 gcgtgaacca gggaggctaa taacaccact tgcaagaagg caaaaataaa cgcaccgata
     1861 atcataatat gcgtcagggg gattttcctt tgctccttca ttttaaataa cccacaaact
     1921 gaccttcctt cattaaggcg acttttcccg tatggtgata aacaatgacg ttataaagac
     1981 ctgactgata aacagtggca tctttattgt cttcatcaat tgcacataat gaatcattga
     2041 gataagcaaa gttcttactg ctcattctaa aggtcatgcc aaatccttta taaatggctt
     2101 tctttgccgc ctgggcatgt tgtgcggctt ctgcattttt aaatgcctgc gcctcggatg
     2161 cacttaaaat agccgtctga cgctgttgtt ctgcgagata acaggtgtca tggctgacgc
     2221 cctgcgcctg gcaatctgcg atgcgggaag tgggtgaact acagccagtc agtaatataa
     2281 ccgccagcag ggaaaacgat ttaatggagc tattcatcat tgttaacctt aaataatgtg
     2341 cgaattaatg aagaggtgaa acccggcaag cttccttgct cttatttata ttgaggattg
     2401 aaatattaga acgaatatcc tacgccgaca cctgcgccga aatcagattg cgtatccgca
     2461 gaaataccgg ctttaagtac cgtatgcgtt ccggcattaa aagatgttcc gacagacacc
     2521 gcagattcac cgttgaaggt tcccgctccc gctgacacca tcatcaagtc accggttttc
     2581 acctgtggtt gtgaggcgat agcgatggct gatgcagttc ccgcattggc ttctttacgg
     2641 ttgtcctcaa cttcgttttt cagcgaactg aaatggttat tgagcgtgtt aatttgttgg
     2701 ctgttatctg cgacccgttt ttgcgtgttg cttaactgct gatgcgtttg ggttgcgtct
     2761 tgtgtcaggc tgtcaatttg ttcatgcgtt tgcgttgagt cttgcgccag acgctgaatt
     2821 tgttccccgt actgcacact atcgatatgt tgttgcgttt cctggttggt cgcaagggta
     2881 tcaattttct gtttttgttc tgcagcagta cgcgcatttg catcattttg tgcgctcagt
     2941 ctggcgttaa ccatttcctg atagtgtgcg ctggtttggg catcgccaag tgcggaaacc
     3001 gttttttgtg tggcgtcgat ttgcttctgc tgatcggtaa tttgttgctg ctgggtgtcc
     3061 gcttgttcct gttgcgcatt ggcagcggct gtctgtctgg cgtcaatcat gctctgatat
     3121 cgggcggcag tctgtacatc accggtggca gcaacggttt tttgggtttc gtcgagctgt
     3181 ttctgttggt cagcaatatc gctgcggttc tgctcgatat tgtttttgtt ttctgtcaca
     3241 gcagaggaga gtttgacgcc gttcatcaga gcggtgttgg cgttagcgtt ggcatggtca
     3301 gccgttgact gggcatgtgc gacatcgcct tttatgttgg tgacgtctga ctgcactgcg
     3361 gagacatcac tgcgggtgtc ggcaatggag gctttattgg tatcaatatc cgtctgcgcc
     3421 accataatgg cgtgctgcgc gtcatcagct ttcagcgatg cccagttggc tgtgtgctgt
     3481 gcgttctgaa tggcggtatc ctgcccattg tcgcggtcga tctgcgcttg attggttatc
     3541 gggttcagcg tactggcggg ctgtacggga gaagaagttc tattttcctg aggcgtcgtt
     3601 gtctggtaat catgcgcaat cagataattt tgtgcggctg cgctattttc tgtcctttct
     3661 aaaaatcgcc caattttctg atcaatttcc atttgcgaca ttcctggctg gattgccgct
     3721 aattctgtcg ctgccgcctc aacagtatct cccgcaaacg cagatggact taccgcggta
     3781 gcaacaacga cagccagtaa agacaacttt acagtattca tgcatttttt ctcgttcaaa
     3841 gtaagtcaca catttgtttc tataagcaac gtaaagtgtt gttatgagaa ttgatctgcg
     3901 ttgcaattta atcgaaatct acgccgccat ttgtgtgatg taaagcgcat ttttgtctgg
     3961 tgt
//
LOCUS       DAZFRG010000339         3962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFRG010000339 DAZFRG010000000
VERSION     DAZFRG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.341"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3740
                     /gene="vat"
                     /locus_tag="ACHKUC_004696"
     CDS             <1..3740
                     /gene="vat"
                     /locus_tag="ACHKUC_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
                     /protein_id="HFO6620790.1"
                     /translation="KGYQSVSFGDGQNSYHIVDRNEHSSSDLHTPRLDKLVTEVAPAT
                     VTSSSTADILNPSKYSAFYRAGSGSQYIQDSQGKRHWVTGGYGYLTGGILPTSFFYHG
                     SDGIQLYMGGNIHDHSILPSFGEAGDSGSPLFGWNTAKGQWELVGVYSGVGGGTNLIY
                     SLIPQSFLSQIYSEDNDAPVFFNASSGAPLQWKFDSSTGTGSLKQGSDEYAMHGQKGS
                     DLNAGKNLTFLGHNGQIDLENSVTQGAGSLTFTDDYTVTTSNGSTWTGAGIIVDKDAS
                     VNWQVNGVKGDNLHKIGEGTLVVQGTGVNEGGLKVGDGTVVLNQQADSSGHVQAFSSV
                     NIASGRPTVVLADNQQVNPDNISWGYRGGVLDVNGNDLTFHKLNAADYGATLGNSSDK
                     TANITLDYQTRPADVKVNEWSSSNRGTVGSLYIYNNPYTHTVDYFILKTSSYGWFPTG
                     QVSNEHWEYVGHDQNSAQALLANRINNKGYLYHGKLLGNINFSNKATPGTTGALVMDG
                     SANMSGTFTQENGRLTIQGHPVIHASTSQSIANTVSSLGDNSVLTQPTSFTQDDWENR
                     TFSFGSLVLKDTDFGLGRNATLNTTIQADNSSVTLGDSRIFIDKKDGQGTAFTLEEGT
                     SVATKDADKSVFNGTVNLDNQSVLNINDIFNGGIQANNSTVNISSDSAILGNSTLTST
                     ALNLNKGANALASQSFVSDGPVNISDATLSLNSRPDEVSHTLLPVYDYAGSWNLKGDD
                     ARLNVGPYSMLSGNINVQDKGTVTLGGEGELSPDLTLQNQMLYSLFNGYRNTWSGSLN
                     APDATVSMTDTQWSMNGNSTAGNMKLNRTIVGFNGGTSPFTTLTTDNLDAVQSAFVMR
                     TDLNKADKLVINKSATGHDNSIWVNFLKKPSDKDTLDIPLVSAPETTADNLFRASTRV
                     VGFSDVTPTLSVRKEDGKKEWVLDGYQVARNDGQGKAAATFMHISYNNFITEVNNLNK
                     RMGDLRDINGEAGTWVRLLNGSGSADGGFTDHYTLLQMGADRKHELGSMDLFTGVMAT
                     YTDTDASAGLYSGKTKSWGGGFYASGLFRSGAYFDLIAKYIHNENKYDLNFAGAGKQN
                     FRSHSLYAGAEVGYRYHLTDTTFVEPQAELVWGRLQGQTFNWNDSGMDVSMRRNSVNP
                     LVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADVHLKDAAGEHQINGRKDGRML
                     YGVGLNARFGDNTRLGLEVERSAFGKYNTDDAINANIRYSF"
BASE COUNT         1091 a          907 c         1014 g          950 t
ORIGIN      
        1 ataaaggata tcagagcgtc agcttcggtg atggtcagaa cagttaccat attgtggatc
       61 gtaatgaaca cagttcatct gatctccaca caccaagact tgataagctc gtaactgagg
      121 ttgctccggc taccgtaacc agctcatcaa cagctgatat attgaaccct tcaaaatact
      181 cggcattcta cagggctggt tcgggaagtc agtatattca ggatagtcag ggtaagcgac
      241 attgggtaac aggtgggtat ggttatctga caggaggaat actcccgaca tcattctttt
      301 atcacggctc agacggcatt cagctgtata tggggggcaa catacatgat catagcatcc
      361 tgccctcttt tggagaggcc ggcgacagtg gttctccatt atttggctgg aatacggcca
      421 aagggcagtg ggaactggtc ggtgtttact cgggagtagg aggggggacc aatttgatat
      481 attctcttat tcctcagagt tttctctcac agatctattc agaggataat gacgctcccg
      541 tcttttttaa tgcctcatcc ggcgcccccc tgcaatggaa atttgacagc agcaccggca
      601 ctggctctct gaaacagggt tccgatgaat atgccatgca cgggcaaaaa ggttctgacc
      661 tgaacgcagg taaaaatctg acattcctgg gacataatgg tcagattgac ctggaaaact
      721 ctgtcacgca gggtgccggt tcactgacat ttactgatga ctacactgtc accacttcaa
      781 acggaagtac ctggaccggg gccggtatta ttgtggacaa ggatgcctcc gtaaactggc
      841 aggttaatgg tgtgaaaggt gacaacctgc ataaaatcgg cgaaggaacc ctggttgtac
      901 agggaaccgg tgttaatgag ggcggcctga aagtcgggga tgggaccgtt gtcctcaatc
      961 agcaggctga cagttcagga cacgttcagg cattcagtag cgtgaatatt gccagcggcc
     1021 gcccgacagt cgtgctggca gacaaccagc aggttaatcc ggacaatata tcctggggct
     1081 accggggggg ggttctggat gttaacggga atgacctgac atttcataag ctgaatgccg
     1141 ccgattatgg cgcaactctc ggtaacagca gtgataaaac ggctaatatc actctggatt
     1201 atcagacgcg tccggcagac gtaaaagtta atgaatggtc atcatcaaac aggggaacag
     1261 taggttcatt atatatttat aataatccct atactcatac cgtcgattat tttatcctga
     1321 aaacaagtag ttatggctgg ttccctaccg gtcaggtcag taacgagcac tgggaatatg
     1381 tcggacatga ccagaacagt gcacaggcac tgcttgcaaa cagaattaat aataaagggt
     1441 atctgtatca tggcaagttg ctgggaaata ttaatttctc aaataaagca accccgggta
     1501 caaccggcgc attggttatg gacggctcag cgaatatgtc cggtacattt actcaggaaa
     1561 acggtcgtct gaccattcag ggccacccgg ttatccatgc ttcaacgtct cagagtattg
     1621 caaatacagt ctcgtctctg ggcgacaatt ccgttctgac acagcccacc tcatttacac
     1681 aggatgactg ggagaacagg acgttcagct ttggttcgct cgtgttaaaa gatacagact
     1741 ttggtctggg ccgcaatgcc acactgaaca caaccatcca ggcagataac tccagcgtca
     1801 cgctgggcga cagtcggata tttatcgaca aaaaagatgg ccagggaaca gcctttaccc
     1861 ttgaagaagg cacatctgtt gcaactaaag atgcagataa aagtgtcttc aacggcaccg
     1921 tcaacctgga taatcagtca gtgctgaata tcaatgatat attcaatggc ggaatacagg
     1981 cgaacaacag taccgtgaat atctcctcag acagtgccat tctggggaac tcaacgctga
     2041 ccagtaccgc cctgaatctg aacaagggag caaatgctct ggccagtcag agttttgttt
     2101 ctgacggtcc agtgaatatt tctgatgcca ccctgagtct gaacagccgt cctgatgagg
     2161 tatctcacac acttttacct gtatacgatt atgccggttc atggaacctg aagggagacg
     2221 atgcccgcct gaacgtgggg ccgtacagta tgttgtcagg taatatcaat gttcaggata
     2281 aagggactgt caccctcgga ggggaagggg aactgagtcc tgacctgact cttcagaatc
     2341 agatgttgta cagcctgttt aacgggtacc gcaatacctg gagcgggagc ctgaatgcac
     2401 cggatgccac cgtcagcatg acagacaccc agtggtcgat gaacggaaac tccacggcag
     2461 gaaatatgaa acttaaccgg acaatagtcg gttttaacgg gggaacatca ccgttcacga
     2521 cactgacaac agataatctg gacgcggttc agtcagcatt tgtcatgcgt acagacctta
     2581 acaaggcaga caaactggtg ataaacaagt cggcaacagg tcatgacaac agcatctggg
     2641 ttaacttcct gaaaaaaccc tctgacaagg acacgcttga tattccactg gtcagcgcac
     2701 ctgaaacgac agctgataat ctgttcaggg catcaacacg ggttgtggga ttcagtgatg
     2761 tcacccccac ccttagtgtc agaaaagagg acgggaaaaa agagtgggtc ctcgatggtt
     2821 accaggttgc acgtaacgac ggccagggta aggctgccgc cacattcatg cacatcagct
     2881 ataacaactt catcactgaa gttaacaacc tgaacaaacg catgggcgat ttgagggata
     2941 ttaacggcga agccggtacg tgggtgcgtc tgctgaacgg ttccggctct gctgatggcg
     3001 gtttcactga ccactatacc ctgctgcaga tgggggctga ccgtaagcac gaactgggaa
     3061 gtatggacct gtttaccggc gtgatggcca cctacactga cacagatgcg tcagcaggcc
     3121 tgtacagcgg taaaacaaaa tcatggggtg gtggtttcta tgccagtggt ctgttccggt
     3181 ccggcgctta ctttgatttg attgccaaat atattcacaa tgaaaacaaa tatgacctga
     3241 actttgccgg agctggtaaa cagaacttcc gcagccattc actgtatgca ggtgcagaag
     3301 tcggataccg ttatcatctg acagatacga cgtttgttga acctcaggcg gaactggtct
     3361 ggggaagact gcagggccaa acatttaact ggaacgacag tggaatggat gtctcaatgc
     3421 gtcgtaacag cgttaatcct ctggtaggca gaaccggcgt tgtttccggt aaaaccttca
     3481 gtggtaagga ctggagtctg acagcccgtg ccggcctgca ttatgagttc gatctgacgg
     3541 acagtgctga cgttcacctg aaggatgcag cgggagaaca tcagattaat ggcagaaaag
     3601 acggtcgtat gctttacggt gtggggttaa atgcccggtt tggcgacaat acgcgtctgg
     3661 ggctggaagt tgaacgctct gcattcggta aatacaacac agatgatgcg ataaacgcta
     3721 atattcgtta ttcattctga tgctgtgcag caaagggact attgctttgt ccctttgctg
     3781 cgattatctg attacggtac aggctggaac ttagattttc cggcgttcac gatacttcat
     3841 gtaacactca ggttgagtaa tcttcatgag ttttcttttg ccgtgtggtg gaattatgaa
     3901 taatatagat ttattaaaaa ctataacaaa ctacaaaaaa atcaaaaatc cggcataccc
     3961 gg
//
LOCUS       DAZFRG010000340         3930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFRG010000340 DAZFRG010000000
VERSION     DAZFRG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.342"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..1712
                     /gene="dpiB"
                     /locus_tag="ACHKUC_004697"
     CDS             54..1712
                     /gene="dpiB"
                     /locus_tag="ACHKUC_004697"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO6620791.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1681..2361
                     /gene="dpiA"
                     /locus_tag="ACHKUC_004698"
                     /gene_synonym="citB"
     CDS             1681..2361
                     /gene="dpiA"
                     /locus_tag="ACHKUC_004698"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO6620792.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2402..3787)
                     /gene="dcuC"
                     /locus_tag="ACHKUC_004699"
     CDS             complement(2402..3787)
                     /gene="dcuC"
                     /locus_tag="ACHKUC_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6620793.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1071 a          937 c         1014 g          908 t
ORIGIN      
        1 aacattttaa ttacatccgt caaagaggct cgggacaacc cgcaaggaaa acaatgttgc
       61 agcttaaaga gaataaacag tttgcatttt tccaaagact ggcatttccg ctgcgtatct
      121 ttttgctgat tctggtgttc tcaatatttg tcattgcagc cctggcgcaa tattttacag
      181 ccagttttga ggactattta acgcttcatg tgcgcgacat ggcaatgaat caggcaaaaa
      241 ttattgcttc taatgacagt atcatcagtg cagtgaaaac gcgtgactac aagaggctgg
      301 cgaccatcgc tgacaaatta caaagagata ctgatttcga ttatgtggtg attggcgatc
      361 ggcactcgat tcgtctttac catcctaatc cggagaaaat tggttatcct atgcagttca
      421 ccaaaccggg cgcgctggag aaaggggaga gctacttcat caccgggaaa gggtcaattg
      481 gcatggcgat gcgtgccaaa acgccaatct ttgatgacga tggaaaagtc atcggcgtgg
      541 tgtcgattgg ctacctggtg agtaaaatcg atagctggcg ggctgagttt ttattaccga
      601 tggcaggcgt gtttgtcgtg ctgttaggga ttttgatgtt gctgtcgtgg ttcctggccg
      661 cgcatatccg tcggcagatg atgggcatgg agccaaagca aatcgctcgc gtagtccgtc
      721 agcaagaggc gctgtttagt tcggtttatg aagggctgat tgcggtggat ccgcacgggt
      781 acattaccgc catcaatcgt aacgcaagaa agatgctggg gctgagctcc cccggacggc
      841 aatggttggg taaacccatt gctgaagtgg tcaggcccgc cgatttcttt accgaacaga
      901 ttgatgaaaa acgtcaggat gtggtggcga actttaacgg tctgagcgtt attgccaacc
      961 gggaagctat tcgttctggc gatgatttac tgggggccat tattagcttt cgtagtaaag
     1021 acgaaatttc caccctcaat gcgcaactga cgcaaattaa acaatacgtc gagagccttc
     1081 gtacattgcg acacgagcat ctcaattgga tgtcgacgct caatggtctg ttgcagatga
     1141 aagagtatga tcgcgtgctg gcgatggtgc agggggagtc tcaggcccag caacagctta
     1201 ttgacagcct gcgtgaggcg tttgccgatc gccaggtggc ggggctgctt tttggtaaag
     1261 tgcagcgcgc ccgcgaactg gggctaaaaa tggtcatcgt cccaggtagc cagctttcgc
     1321 aactgccgcc aggactggac agcaccgagt ttgcagccat tgttggcaat ttacttgata
     1381 acgccttcga agcaagcctg cgtagcgatg aaggaaacaa gatcgttgaa ttattcctca
     1441 gcgatgaagg cgatgatgtg gtgattgaag tcgccgatca gggctgcggc gttccagagt
     1501 ctctacgaga caaaatattt gagcagggtg tcagtacgcg tgctgacgag cccggtgaac
     1561 atggcattgg gttgtacttg attgccagct acgtaacgcg ctgcggtggt gttatcactc
     1621 tcgaagataa tgatccctgc ggtaccttat tttcaatcta tattccgaaa gtgaaaccta
     1681 atgacagctc cattaaccct attgatcgtt gaggacgaaa cgccgctggc agagatgcat
     1741 gcggaatata ttcgtcacat tcccggattc agccagatat tactggcggg aaacctggcg
     1801 caggcccgaa tgatgatcga gcgttttaag ccggggctaa tcttgcttga taactatctt
     1861 cctgacggta gagggattaa tttactgcat gaactggtgc aggcgcatta tcccggcgac
     1921 gtggtgttta ccactgcagc cagcgatatg gaaacggtgt ctgaagccgt acgttgtggt
     1981 gtatttgatt atctcattaa gcccattgcc tatgaacggt tggggcaaac gctaacccgt
     2041 ttccgccagc gtaaacatat gctggaaagt attgatagcg ccagccagaa gcaaattgat
     2101 gagatgttta atgcctatgc tcgcggtgaa cccaaagacg aactgccgac cggcattgac
     2161 cccttgacgc taaacgcggt gcgaaaactg ttcaaagagc ctggtgtgca acatacggca
     2221 gaaacggtgg cgcaggcact gaccatcagc cgcaccactg ccaggcgtta tcttgaatat
     2281 tgcgccagcc gccatctgat tattgctgaa attgttcacg gcaaagttgg cagaccgcaa
     2341 cgcatatacc acagtgggtg acatgataaa gccggaggaa acttgcctcc ggcattacta
     2401 tttacttgcc tgtgaccgct gctgccgttc ctggcaccat cagctctgta gcaacgataa
     2461 caatcaccaa accaacaagc accggcaccg aggtgcgttt tacgacttca aacggcgaga
     2521 tcttcgccat cccggcaacc gcaacgacta cgccagaaac gggcgaaagg gtacggccca
     2581 ggtttgacgc ctgcagcatc gggatagtca aatacgccgg gttaatgccg gaagagtgcg
     2641 ccagtttcgg gatcatctca acaaacgcat aaaacggcgc attgcctgaa ccggtcgtga
     2701 ctgccgccag cattgtcagg atcaccaata ccagcatcag gatgatactc gccgaaccaa
     2761 acgaggtagc gatagaaatc agactttgaa taaagccgat ggtgctaagc ccctgagcga
     2821 ataccccagc ggcaaccagc agcatcacca cgttagcaaa tgcatctgcc ataccgcgat
     2881 aagccacttc cagaccagag aaaactttct gggtattaaa gctgcggatg aactccagaa
     2941 tggaggcaat tagcatacaa atcaccagaa tagtgatgat gtgtaattgc ggaccccatt
     3001 tgccgtcaaa aatcagcact ccgatgatcg gcgtgaacgg caaaatggca taaaacgcag
     3061 gggcagtggt ggtgatttca ctgacatcta acatttcatg agagatgtgc tcttttttat
     3121 ccagataacg ttgccagaag aagtgggcga ttgccatgcc gataattgca gcaattgaga
     3181 taggcagcgt tgttttgaag gcgaagtcaa tcagcgacat ttcggaagcc tgcgccgcca
     3241 gcaccacatc tcctgaagtc ggtgcgagaa taatcgccgc cggggaggca caaatggcag
     3301 cagctgcgcc acgactgata ccaacgttta ccatcaccgg aaacagggtt gccatcagca
     3361 aaacacccag accggttgcg gaagagacag ccagtgacat cagacaggca acaaaatagg
     3421 cggcaatcat cagaaggtaa ggggagttaa tatactgcaa tggttttgac gccagcttga
     3481 ccaccatatc attcgcgccg atatgggtca tgtaagcggc aaagccacac agcatcataa
     3541 tcatcatgcc gaggtcgccg ccgcggctca ttagcaatat tttaacgtat tcaacgatat
     3601 ccgtggcgct gtagcctgtt gaagcctggc tggacggtaa cactttgtgc cccataatgg
     3661 cactgataat cagcaataac aggccaccga caaataacac gccagtggcc gaataccctt
     3721 taatgatgta gcgagctaca cccacaataa ccacaacccc aataaggagt tcaatgaatg
     3781 tcagcataat ttttcctgtc tccaggcccc aaagtaaata ataaaaaatt cctaaaagtt
     3841 aaggaaaaaa tatgcccaat aaattggcgg tgaatgctga ttaaaatcaa gaaaaactgc
     3901 cattaagaca ttgaagttgc tgtttttatg
//
LOCUS       DAZFRG010000341         3856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFRG010000341 DAZFRG010000000
VERSION     DAZFRG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.343"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..519
                     /gene="ykgH"
                     /locus_tag="ACHKUC_004700"
     CDS             <1..519
                     /gene="ykgH"
                     /locus_tag="ACHKUC_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO6620794.1"
                     /translation="YSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSP
                     DVKNREIKLEWSGNKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINS
                     ELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVK
                     KMVDEYQQNSGQ"
     gene            complement(762..1457)
                     /locus_tag="ACHKUC_004701"
     CDS             complement(762..1457)
                     /locus_tag="ACHKUC_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6620795.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1450..2877)
                     /locus_tag="ACHKUC_004702"
     CDS             complement(1450..2877)
                     /locus_tag="ACHKUC_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6620796.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2888..3607)
                     /locus_tag="ACHKUC_004703"
     CDS             complement(2888..3607)
                     /locus_tag="ACHKUC_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6620797.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYTKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1004 a         1030 c          811 g         1011 t
ORIGIN      
        1 tacagtctaa aaataaacca gacaacacca ggcatacttg ttagctgtga tagcaataat
       61 aactttgcct gtcagaccac aatgactgaa gatgttattc agcgaattac tacatttttt
      121 cagaccagcc cagatgtcaa gaacagagaa ataaagctgg aatggtcagg aaataagaga
      181 gatttaccaa ctgctgaagc ggaaatatct cgcgtgcagg cgtctatcat caaatggtat
      241 gcgtcagaat accataatgg caggcaagtt ctcgatgaga tacaaacgcc ttcagcaatt
      301 aacagtgagc tttatacaaa aatgatatac ctgaccagga actggtcact gtatccgaac
      361 ggtgatggct gtgtcactat aagctcacca gaaataaaaa ataaataccc tgctgccatt
      421 tgcctggctc tgggattttt tctaagtatt gtgatttctg tgatgttttg ccttgtcaaa
      481 aaaatggtag atgaatacca acaaaactct gggcagtaat aaacagcatt ctgacagaga
      541 gtgcctgtat aaaactatag tgacagtgac cgggaaggga gcccgtcttc aggaatggat
      601 tcctgtcttt ttaactgtaa atatcctggc attattaatg tcaggatatt tacagaagag
      661 ctgcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat tcacaacctc
      721 gtataccgcc ttcaggttta tgtccagact acatatctct ctcagcaatc ctcaataatc
      781 agatacaccg cttttaccgg accatgaacc ccgacgactt tgataagctc aatgtccgcc
      841 gttgaactgg gaccactgat gatgttaata catgacggca ttcgttcacc ggcctgcgct
      901 ttctgatgca atttttctgc gagttgcgct acacgcggca ggattgtgct tttacgcagg
      961 ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgcccggc ggcggaaaaa
     1021 agaaccacgc ctcccgattc ggttaaacca tattcagcat atacgacgcc cactttagcc
     1081 tgctctgctt gtgatatgtt ctcaatacct ttcgccggat cccaaacaac ggcattgcat
     1141 tcctgctgca aacgttcgct aatccccaat tcctccagcc tcgtgtcacc actaatcacg
     1201 accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc cttcgcctcg
     1261 ctggtcagtt cacagcgcgt caacataaca tcgctggcaa actggataaa cgcgtcacag
     1321 cgctgctgtt gggtaagttg ggtaagccgc tcgttagcat agttgttaag cggcgcatct
     1381 tctgcttgcg gttcaagtcg cagcgggcgc cccagtgcct gagcaacgtt attcaaaaat
     1441 tcgctccgat tatccattct ttttctcctg cgcctgatgt ttcttaaacc aactacggaa
     1501 actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa
     1561 tttgagtggt gttttgccgc cattgataaa ccagcttgcc gcatgagcac cagccatcat
     1621 cccaactttc cacaatcctg gatgactatt ggcataagcg aacattttta tcgcccgttg
     1681 ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca aaatcagttt
     1741 tgacagcgga atacgcaccg gacacacgct gtcacaagcg gtgcataaag agcaggcgta
     1801 gggtaaatct ttaaaatctt tatagccgcc aagtaacggg gaaatcaccg caccaattgg
     1861 ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg gacaagtatt
     1921 catacaagcc ccgcaacgaa tacagcgcag cacatcccga aattcagagg ccagcacctc
     1981 agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa cgtgccccgc
     2041 ttcgcgcggt cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg
     2101 cgccagcatg gtgatcaata catctacctc tgcaaacgtg ggagcaatac gctccattcc
     2161 catcactgca atatgcgttt taggcagcgt ggtacacatt cgcgcattac cttcattggt
     2221 caccaggcat accgaaccgg tctctgccac cgcgaaatta cagccggtaa tacctatttc
     2281 agcactgagg aaatcttcgc ggattttttg ccgaatgaat aaggtcatcg cttcaggcgt
     2341 ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat ggcgatcttt
     2401 atgaattgcc gggaccacaa catgagaggg cggatcttga tccagctgga gaatgtattc
     2461 acccagatcg gtttcaatca cctgaatccc ggcatcctgc aacacatgat tgacaccaat
     2521 ctcttcggtc accatcgatt tagatttcac caccttccgg gcatttttgc gttgggcaac
     2581 ctgtaaaatg tagcgggtgg cgtcttcttt ggtttttgca aaatagacgt gacctccgtt
     2641 ttgcgtcact ttttctgaga gctggtacag ataagcgtcg agattactca gaacatgatc
     2701 acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat cgaccatttt
     2761 ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttacgca tgatcggatc
     2821 ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga tcgacatctt
     2881 tatatcctca gcggctcatc agcacttcag caatatgcat cactttgact ttctgccctt
     2941 cccgttgtaa tcgtccactg atgttcagca ggcaactcac gtcagcacca attaaatact
     3001 cagggcggac ttccatcagg tgcgcaacct tttctttcac catctcgccg gatatttcgg
     3061 ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca
     3121 acagctccag tccacgcaca tttttcagca gcgtaagagg ttcgtccttc acccccagct
     3181 tacgggccag gctacaagat gggtgataca ccgctctccc ttgcaaactg gcacctacat
     3241 cgactacccc taatgtatta acaataaaag aggtgagatc ctgcatacgc gcggcaacct
     3301 tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa ctttttacgg
     3361 cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga
     3421 tcagattttt catccctgga atcgcttctt tggtataacc actattgatc gcaggctgac
     3481 cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc agtagcagca
     3541 cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca aagaaattga
     3601 cattcacaac taatactcca ttacttcatg cccatttatg cccgatggtt ccccccttcc
     3661 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3721 ctcatttgta tgacaaatga taatctttca tctaaacttc aaatgcaata actaatttta
     3781 tttctttcgg catcgctcac attttatagg gaaatcgacg atcatttttg gcataagaaa
     3841 tctatttaaa aattgc
//
LOCUS       DAZFRG010000342         3836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFRG010000342 DAZFRG010000000
VERSION     DAZFRG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.344"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..690
                     /locus_tag="ACHKUC_004704"
     CDS             <1..690
                     /locus_tag="ACHKUC_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO6620798.1"
                     /translation="TEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQSPVILKKI
                     QTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIIDTPLLRKL
                     NQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAEDMN
                     SEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFDAT
                     NSELCAERHEC"
     gene            617..2739
                     /locus_tag="ACHKUC_004705"
                     /pseudo
     CDS             617..2739
                     /locus_tag="ACHKUC_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2909..>3836)
                     /locus_tag="ACHKUC_004706"
     CDS             complement(2909..>3836)
                     /locus_tag="ACHKUC_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO6620799.1"
                     /translation="AIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTM
                     TMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLT
                     MKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEM
                     RSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDC
                     YNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAM
                     ADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1115 a          885 c          876 g          960 t
ORIGIN      
        1 acggaagcag ttgagatcac taccaaagcc ataaaacaaa aaattactcc tagcgatatt
       61 tatcgtcatg ccttgagtgg caacatccta ctatcggtct atttccagtc tcctgtgata
      121 cttaagaaga tacaaacttt taatggaaaa ataaaattca ggcagtttgt gggagaccta
      181 cttgataaac tatgcatgct tgacagggac ggttttattt atggtcaaaa tttaagactc
      241 tgtacagaag ccagatatat ttgtcctgtg caacaaatta tagatacccc cctgttacgc
      301 aaactcaacc agtttcgtac gtttgttcgt aatgtccgcc cgggtgatga actggatgtc
      361 ccggcacaag ttagtgaaaa aaatttaacc ccgccgccgg gtaacagcag tggcaacctt
      421 gagcaacaga tagccagtac ttcacagcta attggatctc tgctcgccga agatatgaac
      481 agcgagcaag cggcaaacat agcacgtgga tgggcctctt ctcaggcttc aggggtaatg
      541 acagactggt taagccgctt cggtaccgca agaatcacgc tgggcgtgga tgaagatttc
      601 agcctgaaga actcccgtga cggcaaccct cgaaaatggc gattcgatgc aacaaacagt
      661 gaactatgtg ccgaacgtca cgaatgctga aatcacgctg gcagcctcga aggatccggt
      721 gattgccgac aataacgatc tcacgacact aacagcaccg tcgctgatac agagggcaat
      781 gcgatagcca acactgaggt aacatttact ctaccggaag atgtgaaggc gaacttcacg
      841 ctgagcgatg gcgacaaagc ggttactgat gccgatggca aagcgaaagt cacgctgaaa
      901 ggtacaaaag caggcgctca taccgtgaca gcatcgatgg ttggcggtaa gagtgagcag
      961 ttggtggtga actttactgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
     1021 gacaatttca tcgccaataa cattggaatg accaggctac aggcaacagt gactgatgga
     1081 aacggcaacc cagtagaagg tataaaagtt aatttccgcg gaacctccgt cacgctaagc
     1141 agcaccagcg ttgaaacgga tgatcaggtt ttcgctgaaa ttcttgtgac aagcaccgag
     1201 gtcggactaa aaacagtttc agcctctctg gcagataaac ctactgaagt catctcgcga
     1261 ttactgaatg ccaaggtaga tgttaattct gcgacgatta ccagtcagga gatacctgaa
     1321 ggtcaggtca tggtcgcaca agacatagca gttaaagctc acgttaacga ccagtttggc
     1381 aacccggtga cgcatcaacc cgcgacattc agtgcagcgc catcctcgca aatgatcatc
     1441 agccagaata cggtctctac taatacgcag ggagtagccg aggtcaccat gacgcctgaa
     1501 agaaacggct cgtatactgt aaaagcatcc ctagcgaatg gagcttcact tgagaaacaa
     1561 ctggaggcta ttgatgaaaa actgacactc acgtccagtc cgcttatcgg tgtcaatgct
     1621 cctaaaggcg ctactctgac ggcaacgcta acctctgcaa atggcactcc agtggagggt
     1681 caggtcatca actttagcgt aacgctagaa ggtgcgacgt taagtggcgg aaaagtgaga
     1741 accaactctt caggtcaggc tccagtcgtt ctgaccagca ataaagtcgg tacatatacg
     1801 gtgactgcat cgttccataa cggcgtcaca atacagacac agacaaccgt gaaggttact
     1861 ggcaacccaa gcaccgcaca tgttgctagc tttatcgctg atccgtcgac tatcgccgcc
     1921 accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg taacctgatc
     1981 gaaggtctca ctgtgtactt cgccttaaaa agcggctcca ccacattaac gtcattaaca
     2041 gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggtgagat aacggggagc
     2101 gtcactgtaa gcgcagtcac aagcgcaggt ggaatgcaaa cagtagatat atcgctagtg
     2161 gcaggcccag cagacgcctc gcagtccatc cttaagaaca accagtcatc actgaaaggg
     2221 gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg caatccgatc
     2281 aaagtttctt aagggataga atttgtgcaa tcaggtacaa acgtgcccta tatgaaaatt
     2341 agcgcaattg attacagtca aaatatcaac ggtgattaca aagctactgt tacaggaggc
     2401 ggagagggta tcgcaacgct gatccccgta ttgaatggtg ttcatcaggc gggtctgagt
     2461 accacgatag aattcattag cgcggaaacc agaccaatga cgggtacagt gtcagtgaat
     2521 agtgcaaacc taccgacagc ttcattccct tcgcagggat ttaccggggc gtattatcag
     2581 ttgaataatg acaactttgc accaggaaaa acggcggctg attattcgtt ttcaagctcg
     2641 gcctcctggg taggcgttga tgctactggt aaagtgacat tcaaaaatga cggtgatagc
     2701 aatactgtaa taatcacagc gcccccaaga tccggtggtg ctatttacca aacagttcct
     2761 cctgaatcgc gttcggtgtg actatgatca ttttcggtga gtattgcttt gatccaaagg
     2821 ctggttttct gtgatgattg accagcctca gagtattgat gacaccaccg ttcataacag
     2881 atcggcatta ctatgcataa gtactgcctc aaattctcgc caaaacactt tttaatgcct
     2941 ttgcgaattc atccagactt atattgtcaa ttttttgccc aacgacgact gactccgaaa
     3001 catcggccat tgcgggccat cgcgttttgt aatcagcata aattgcaaca acaggggtat
     3061 tataggcact tgccatatgt aatattgagg tgtcaggagt tattgcaata taagcatctt
     3121 ttatgattgc tgctgaacgt ttaatcgagg gtgataagga taaacggtag acattattat
     3181 aacaatccac taatgccctg gctttatctt cccctttggg tccataaacg atcactattg
     3241 gaatatctgt ttcactttga atttttgcta ttagattttc tgcaatcgat aatgaaaatg
     3301 tacgttcctg cgagctaccg tctaaattga gcgcaatgta agatcctaac gatcgcattt
     3361 cctcgcgtac ctcatcgagt acatcatcgc ttaatggcaa ttcatatatt gacctgactg
     3421 ccggaaaacc cgcctcacgc atcataaatg cccatgtcat gggtacagga gtccggagat
     3481 tctgatccat ccgggacgcg ttcttacaca aaggggaata gcacttcatg gttaaaccga
     3541 cgacctggaa attcgttttt gctttcaact gactgataaa tatcatcgtt ttcaggttct
     3601 ttttacgcat cgcctctata caaagatccg gcgtaccgta ttgcagtgtt atgttcttcg
     3661 ctaaatcttt tatttctttt aatgttgcgt gatcctgcat agtcattgtg actaatgtta
     3721 atttagtctg ttcaagtttg agcgcattaa agacttctaa attaattgtc gacgtaacaa
     3781 ttaatagatg cttaatttta tgcaattcaa gcgcccgaat aacaggaaag atggcc
//
LOCUS       DAZFRG010000343         3784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFRG010000343 DAZFRG010000000
VERSION     DAZFRG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.345"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..1107)
                     /locus_tag="ACHKUC_004707"
     CDS             complement(97..1107)
                     /locus_tag="ACHKUC_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO6620800.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLIAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            complement(1118..2134)
                     /locus_tag="ACHKUC_004708"
     CDS             complement(1118..2134)
                     /locus_tag="ACHKUC_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO6620801.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            complement(2131..3603)
                     /locus_tag="ACHKUC_004709"
     CDS             complement(2131..3603)
                     /locus_tag="ACHKUC_004709"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6620802.1"
                     /translation="MSGNESIDAAHYDQVTTWPRGGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKATGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKV
                     SAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITRQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
BASE COUNT          996 a          902 c          859 g         1027 t
ORIGIN      
        1 cgtcagagga cgaagtgctt tcatatcctg ctcctggtta atataacaga aacgataaat
       61 acagcgcttt tctatcgtta taataaaaaa tagtgattaa ttctctgcca gataatggaa
      121 tattcctgcc ggaaccggaa taccttcctg ctggcatttt tctttgtagt gcatttgcgg
      181 atcgttcggc gctaacactt tttcgacacc aggagccggt ttaacggcat gcagctccgt
      241 gaccattttc gccatagttt ttgcaaaaac aggattgccg agctttttcg gatcgatggc
      301 aataaccaaa cttgccagct tacgcatctt gtcataatca ccatacatgc gaacaatatg
      361 gttgccaaaa ttcgcattca tcagcacgcc agtcagtgca tcaatagcca gtgcaatgcc
      421 tgaacctttg tgttggccga aaggtagcag gttttcaatc ttatacggat cagtggtgcc
      481 ataaccgttt ttatctatcg ccagcccttc accaatatgt tttccggttt cctttgcatg
      541 aagtattttg ccgaaagccg tggcactggt cgccatatca acaatcatcg gatggctgtt
      601 ttccaccggg aaaccaaagg cgatggggtt agttcccaaa aagcgctcgg cgccgccatg
      661 gggagccaca caggtatcgg tttgcgtcat tacgatagca atcaaccctt tgttggtgat
      721 catctctgca aaataagata acgcaccaca gtgagacgtg tttttaacgc taacaaaacc
      781 gagaccctct tgctgggcca gtttaatagc gtgctcagtg gcgctaatta atgcggaatg
      841 ccccattccg tcgtcagagt cgagaatggc cactgacggt gaaatttgct caatgctaaa
      901 ctgcggggct ttatttaacc ctccggcagc aaggcgggtg caataatgtt cgacacgcat
      961 aacaccatgt gaatgcacac cggtaatatc ggcatgtact aaaacatctg tcacctgttg
     1021 tgtggtgtcg gtatccagcc cggcggtatg taatttgtgg tgcaccagtg attttaaatt
     1081 ttcttcactg acataaacag ttgtcatatt tgttccctta aaaagtgatc acacccttga
     1141 ccatattttt attttctaca acgttacgct ggtaatcttc gccaacacta aagaaattga
     1201 acgcctggtt tttcattaaa ttttcgtgaa ttttattgct gcgcatcagt tcaatcacca
     1261 acgcaaaatc ttcccgtgtg gcattgcggc tgctgagtaa cgttgtctct tttttatgga
     1321 aggtcggatc gtcaataaca agttcaccaa tatacaaacc gatgaaaaca attttgccgc
     1381 catgacgaat aagattgaca tcatgactca ttgaagcttt atttcccgtc gcatccagga
     1441 ctacgcaagc cagttcgccg ccaaacactt cactaagcgc ggcaataaaa ccttcctgtg
     1501 ttgggtcgaa gacattaatt gccagatgat ccacaacgtg ctggcgacgt tgacagtcaa
     1561 tatcagcaac aacaacatgt gcccctttag ccctggcgat ggctgcggta gccagaccga
     1621 ttggcccagc gccaatcacc agtacgtttt gttcagcctt gatctctgcc cgacgaacgg
     1681 catgtgcacc aatggcgaaa cattcaacca atgctccggc actgtcgctg acctcgtcag
     1741 ggagaggcac aaggttgtct tcacgtaccg caaggtactc actaaaaccc ccatcctgat
     1801 gcacgccata cagcgaaaca cgttcgcagc agttcgtttt ctcttcccga caggctgcgc
     1861 actcgccaca cggaatgcat gggatgacgg aatagcgctg gcctgattgt gctgtttggc
     1921 actggctgcc tcgcgaaacc gcttcggcgc atatttcatg acctaataca cgtgggtagc
     1981 taaaaaaagg ctgtctgccg gcaaaagcgt gaatatcagt accacaaata cccacagctt
     2041 tgatttttaa cagcacttca ttatctgctg gtgtgggaat atccttttcc acatattcca
     2101 taacgccagg ctgctgacaa attaatgttt tcatttctgc tcatccgata ataattggct
     2161 gatggtggcc ttgatgcctt tgttttttat atttgcaaag gcgattttta tttcatttat
     2221 aaatggctga tggttgctta aatcaccaaa tatggcgcta aggtgcagta atgcgagcgc
     2281 ctgttgccat gcgtcttgtt gttcctttat tagcgcctga agttggtcat tcattggatc
     2341 agcgacagac tggcttttct caacagcatc aatgacataa tgcaaccaac cagcaacgag
     2401 cagtgacaga acagcattgt tttgtttttg ctgatgcaat ttcagccagg gcgtcagcgc
     2461 tcgctgggga agcttttgcg aaccgtccat agcgatttgc ccggtacggt gtgcaacatt
     2521 acggttgcta aagcgttcaa ttaacaggtc ggcgtattcc tggatgttga tatttaagtc
     2581 tggattaagt gttcgggctt gttcattaat catcagttgg cgggtaatgg aacgaaaatt
     2641 agcgtcttcc atgcattgcc agataaactc atagccagcc agtgagccgt tataagccag
     2701 aaatgaatgg ctaccattta acatgcgtaa cttcatctct tcataaggct gaacattgct
     2761 gacaaacatc gcgctgactt tatcccattc cggtcgtccg cgcacaaaat tatcttcgat
     2821 cacccactga cgaaatgatt cgcagacgat cccgcaggga tcggcataac cggttttagt
     2881 ttccagtaag gcgaattgcg attcagtcat tgccggaaca atgcgatcga ccatcgtgcc
     2941 tggaaatgag acattttctt cgatccactg agccaggggc tggtccagtt gtcgggcaaa
     3001 accgagaacg gcattgcgcg tcagatgccc attgtctggc aagttgtcac aggagagcac
     3061 actaaaagcg gcgagtcctg tcgctttacg ttgttgcaaa gcacagacga tcacgccaat
     3121 agctgacaga ggcgcatccg ggttttgtag atcgtgttgg attaatccat tggtgagatc
     3181 aagtgaacgt gtttgcggat cgaggcaata acctttctcg gtgatggtta atgaaacgat
     3241 ttttacctga ggttcagcca gtttatggat tgctgcggca atgccatctc tgggggtatg
     3301 aataccgcca gccaccgaac gcacaaggcg tgaggtagat tgcgatgcag atgtttccac
     3361 aaccgaaaac agcccatttt gcgcatttaa gttttcgatc agttgagcat caccaaacag
     3421 gttaatctca aagatccccc agcgggtgtc cgaaagttga ttagtgagat ccgtgtagac
     3481 agcctggtgt ccgcgatgaa aagcgccaaa accaatatgg acaatatctg cctgtaaccc
     3541 gccccgaggc caggtggtta cctgatcata gtgggcggca tcgatacttt cgttccctga
     3601 cattatgctc ttactcctgg tggcttgtgt gtataccaat ttacataatt cattagacca
     3661 aaaacagagg gatgcatcac gcttgcagag aaaggtaaaa gtacgaaaca gaacaacaag
     3721 gcattattta tatctgtatc aatgagttat atgaataaaa tgaaaggaaa gttttttaga
     3781 ggtc
//
LOCUS       DAZFRG010000344         3771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFRG010000344 DAZFRG010000000
VERSION     DAZFRG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.346"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..1072
                     /gene="ydcK"
                     /locus_tag="ACHKUC_004710"
     CDS             92..1072
                     /gene="ydcK"
                     /locus_tag="ACHKUC_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO6620803.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTPLAGLL"
     gene            complement(1064..1603)
                     /gene="rimL"
                     /locus_tag="ACHKUC_004711"
     CDS             complement(1064..1603)
                     /gene="rimL"
                     /locus_tag="ACHKUC_004711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO6620804.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(1666..1890)
                     /locus_tag="ACHKUC_004712"
     CDS             complement(1666..1890)
                     /locus_tag="ACHKUC_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO6620805.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(2030..3685)
                     /gene="opgD"
                     /locus_tag="ACHKUC_004713"
                     /gene_synonym="mdoD"
     CDS             complement(2030..3685)
                     /gene="opgD"
                     /locus_tag="ACHKUC_004713"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO6620806.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
BASE COUNT          980 a          896 c          917 g          978 t
ORIGIN      
        1 tatttgaaat gcattttatg gaattttctc gagcatagcc agagccgcag aatttgctac
       61 ggttatagta agtcacctgt gggaaaacgc tatgcgtaaa tatcgtctaa gtgaagaaca
      121 gcgagccttt agttatcagg aagatggcac taaaaaaagt gtgttattac ggcaaattat
      181 cgccatgagc gattttaacg atgtgatagc aggaagcgct ggcggctgga tcgatcgcga
      241 aacggtgtta gcacaggaag gaaattgctg gatttacgac cagaatgcta ttgcgttcgg
      301 cggaacggtg atttccggga atacgcgtat caccggcacc agcgtgctgt ggggagaggt
      361 ttacgcaacg gataatgtct ggatcgataa tagtgaaatc agccaggggg catatataag
      421 tgacagcgtc accattagtg actcattagt ttgcggtcaa tgtagaatat ttggtcacgc
      481 cttaattgat caacattcta tgatcgtcgc cgcacaaggc ttaacgcccg accatcaact
      541 cctgctacaa atttatgatc gcgcgagggt tagcgcctcg cgtattgtcc accaggcaca
      601 aatttatggc gatgccgtcg tccggtatgc ttttattgaa catcgcgccg aagtttttga
      661 ttttgccagt attgaaggta atgaagagaa caacgtctgg ctatgcgatt gcgctaaagt
      721 ttacggtcac gcacaggtga aagctggcat agaagaagat gccattccga cgattcatta
      781 cagttcacag gtggcggaat atgccattgt ggaaggtaat tgtgtgttaa aacatcatgt
      841 cttgattggc ggaaatgccg tggtacgtgg cggaccgatt ctgctcgacg agcacgttgt
      901 catacaaggt gagagccgta ttactggtgc ggtgataatt gaaaaccatg tggagttaac
      961 cgaccacgcc gtcgttgaag cctttgatgg cgacacgatt catgtccgtg gtccaaaagt
     1021 cattaacggc gaggagcgca ttactcgaac gccgttggca gggttattgt gaatcgataa
     1081 tacgcgcgta taagttcaca tcatcatagg catcattcag gaactcagcc tgtttcaggc
     1141 aaccttcaag gataaaacca ttgcgcaaag cgacctggtt gctttgcgca ttgtccaccc
     1201 gacatttgat cacgaagcgt ctaagttcac cagactgagc gtaatgatga atcaatgcct
     1261 gcagcgcctg agaaatgatc ccctgcccct gatgagattc gtcgagccag tagccgattt
     1321 cagcggtttt attcagtggt tcaatacgat taaaagagat aacgccgata agttcatctt
     1381 ctttgaaaat catgaacatt ttggcatagc cgcgttgatg caacatcaca ttaccctgca
     1441 ccgtttttcg cgtgtcctct tcactttgaa caaactgcgg ccagtttagc gactgctgta
     1501 accaggtttt atttttacag attaactgat aaagaggtgt gacatgactt tccgcaacag
     1561 catgtaattc aagtgattcg cttactttta tcgtttcagt catcttatct ccagtcaatc
     1621 catgcagctg ttaaaaaagc cgcctggctg gcggcttagg aaagtttaca cctctttgac
     1681 gctcacctgc tgcaggattt tgagcatctc atcttttaac tgtagctttt gttttttcat
     1741 gcggaccact tccgcattgt accctcgacc gtcggaacct tcctttctgg caatttcatg
     1801 atcaagttta ttgtgtttat cgaacaagga cataaagcga ggattttcgt ttttcagacg
     1861 ggatattaaa tctcggtatt ctggaaacat acgcactccc tgttagtggc tgacaaggtg
     1921 tatctgaaat acacaccatc agattactca tttaacttag ggaaaaatgc gagcgggatc
     1981 gtgtttaacc agacggcacc tcccgaagaa gatgccgaag aaaaaacgat taactcatca
     2041 cgcggtcatc aacatactga cgtttatccg gcgctggcgg gaaatactga tacagccatg
     2101 tttcactgat agcgtcgccc tgacaacgca gatacatccg catatcgacc ggatcagtgg
     2161 agtccgaagt cggataccag tcaaactgaa tacgataacc atcaatgggt tcaatataga
     2221 gaatttcgat ttgtttcgct tccccactgg aaagcgtaat caccggctca atgccttttg
     2281 gcgcggcagc tttcagatca ccaccaacga aatcgacggc aaaacgtcgc gcccattttt
     2341 cgggatagtg ttcacctggc gcccaacctt ccgggaaacc gcccatgccg gtacgcgtcg
     2401 ccataacgcg cgctaatggg caatgaacag gcggttgcgc actccagtac agacgatact
     2461 ggaatgcaaa ctcatcaccc gcttttaccg ctttttctgg ctgccagaag cagacaatgt
     2521 tatccagcgt ttcgcccgtt gttgggattt ccatcaggcc gatggtgccc ttaccccact
     2581 tgttacgcgg ttccacccac agacttgggc gtttgttata ccagcccata atgtcctgat
     2641 aatgggagaa atcacgatcc agttgcaata aaccaaaccc tttcgggttg ttgtcggtgt
     2701 aagcattgaa ctgcaatttt tgcggattgt tcagcggacg gcaaatccac tcgccgttgc
     2761 cccgccacat ggacagacga tcagagtcat ggatttgcgg atgaatggtg tcgcacatcc
     2821 gacgttcatt agtaccgcag ctgaacatac tggtcatcgg tgcaatgccc agctgtttaa
     2881 tgtctttgcg cgcatacagg tgattttcca catccataat cacctgattt ttctcacaat
     2941 ggatagtgaa cttataggca ccagtaatgc tggcgctatc gagcaacgca taaacggtaa
     3001 aggtggttgc ccctggtttt accgtatcaa accagaaggc ggtaaagtcg gggaactctt
     3061 ctttgctgtc ggtgtaagtg tcgatcgcca ggccgcgggc cgacaaaccg tattgatatg
     3121 tgtcatcaac ggcgcggaaa taactcgcgc cgaggaatga tactacatca cggcgcgcca
     3181 gttcgggggc tttaaacacg cgaaaaccgg caaaaccgag atcgctttgc ccttctaatt
     3241 gtttggtatc cacacccgca tcgttgtatt tgaacaactc cgggcgaaag tgaatttcac
     3301 gcgccagatg tgttgctgga tctacagaaa acatacgaac gcggcgacgg aatcccattc
     3361 ccatatggaa aaactgagcg tccagttgac ggttttcaac gttatgccag agcgattttt
     3421 cggcgtcgta ttgaatactg ttataagcct gcggcgtcat tgtcgccaga gtatcaggta
     3481 acggacgtgg tgcaccacgc catgctgttt gcgctaagtc gtgcgccatt gactgtagaa
     3541 tggagaagtc aaaacgctgg gtttgcccgt cggcaatatc agaatcggcc gcgaatgccg
     3601 cctgagaaaa aagagaagca atgccgctgg taccgcacac ggcggccata gccattgaac
     3661 ctttaataaa tcgtctacga tccatacctg aaagtgagtc cttctggtgt tgtgaatggt
     3721 cccgcgctgg tcattttcag cggcaagaaa gtgcttattt atgaccgctc a
//
LOCUS       DAZFRG010000345         3720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFRG010000345 DAZFRG010000000
VERSION     DAZFRG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.347"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..828
                     /locus_tag="ACHKUC_004714"
     CDS             121..828
                     /locus_tag="ACHKUC_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6620807.1"
                     /translation="MENLYISGRKVDSFGDWSPQTEHPAITIQRKEHDERIKLEQSLK
                     MSPKVISFVSPEPQEMPDICKSEALFNLEREYYPQLKAQRIKLDDAYSKVTLMQSAIE
                     PSEFEIQDELSQKPFVYYKYEDNDGFGTFPENIPDVINNLSDGYRIVKMVKASRGAGQ
                     FIYMTNKSDEELAELARQNILASRNKQLDSAKLYLSRELQAMKELVSEYESQKKVAMQ
                     ADIEQLTKISSKYAKAI"
     gene            837..1145
                     /locus_tag="ACHKUC_004715"
     CDS             837..1145
                     /locus_tag="ACHKUC_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620808.1"
                     /translation="MSDLTERDKRIKGVMEPFEDRFNEIKTTLGDIQEVTEYLIGRMD
                     EKPLAELKFEGRDDLTFLIRDLKRDVGRLESIFDKAGLSIRYEIVKNERGVKCIHFLE
                     "
     gene            1168..1374
                     /locus_tag="ACHKUC_004716"
     CDS             1168..1374
                     /locus_tag="ACHKUC_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620809.1"
                     /translation="MIDLTERYELIRAISERLKEQPNKPKRIKSSTSKQNNRIRRIAK
                     WCLDKLPSDCQIVGEDGNYYLVRH"
     gene            1557..3605
                     /locus_tag="ACHKUC_004717"
     CDS             1557..3605
                     /locus_tag="ACHKUC_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6620810.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQKVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTASLKSMEGKINKTFDRLEARAKNTKAFRFTSQVNDAINPPKQPRQ
                     PRISGNRAITAAYSANMSKLKGLNPIVEKYIKSQFYALSSKAGQMDNTKFNEALAKLN
                     SSVREAIAKARGHTSTSINGDRAASSLDSLSSAAVKTAGAFISFQAVLSAYQRVMEVG
                     LQRASSERSIDFVFGDQSTQVKKFIQDLTQITGMDTTETQAQFASFGASATTTMGLEG
                     SEELFKNLTGYARLMGRSDEQIQRALTAVSQMASKGQVMAEELKGQLSEALPGATQAF
                     AKALGLTEKQLFDKMKNGDVKASDALPKFAKELGAQINSRGGWKAIQDSTQTMLGNLK
                     NTWNNSLTDIFRGSENGLQDFTRSLTTLLNSLGGSGKSLGESLGSLMTDMSHGVDSLT
                     DISYRVRAFFDEVTLAYRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSI
                     YGLMTAITRLGQVADTTADNVNRKNGKPSRFNLGNMLLAGSAIYAGYERLASTPERME
                     AQAKLPELAKESGLKPVFTMDDVKGWFNSAAMNLANFQHPELITMRQDAALTNTDIQS
                     LRDEISALSKRIQEPVKVSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD
                     "
BASE COUNT         1235 a          635 c          825 g         1025 t
ORIGIN      
        1 ctagggcttt ttcagctttt aaaaagcata catggcaaac gcttttaagt ctcttctaag
       61 ttcgtaggct gtcaggtgtg ctttttaaaa actgaatatt acttagaaga ggaaaatgaa
      121 atggaaaatc tatatattag cggtagaaag gttgatagct tcggtgactg gtcgccacag
      181 actgaacatc cagctatcac tattcagcgt aaagaacacg atgaaagaat taagcttgag
      241 caatcgttaa agatgtcacc aaaggtaatt tcctttgtgt ctcctgagcc tcaagaaatg
      301 ccagatatct gcaaaagtga agcactattt aatcttgagc gtgaatatta cccacagtta
      361 aaagcacagc gtattaaact tgatgacgct tacagcaaag ttaccctgat gcaatcagct
      421 attgagccgt cagaatttga gattcaggat gaattgtcac agaaaccatt tgtctattac
      481 aaatatgaag acaatgatgg ttttggtact tttcctgaga atatcccaga cgtgattaat
      541 aatctttctg acggatatcg aattgtgaag atggttaagg catcaagagg tgctggtcag
      601 tttatttaca tgactaataa gagcgatgaa gaactcgctg aacttgctag acagaacatc
      661 ttggcatcac gtaacaagca gcttgatagt gctaagttgt atctctcaag agagcttcag
      721 gctatgaaag agcttgttag tgaatatgag tcgcagaaga aggttgctat gcaggctgat
      781 attgagcagc ttacgaagat ttctagcaaa tatgcaaaag ctatttgagg tgattaatga
      841 gtgatttaac agaacgcgat aaaagaatta aaggggtgat ggaacctttt gaagataggt
      901 tcaatgaaat taaaacgact cttggtgata ttcaggaggt cactgaatat cttattggtc
      961 gtatggacga aaagcctctt gccgaactaa agtttgaggg tcgagatgat ttaaccttcc
     1021 ttattagaga cctcaagcga gatgtaggac ggcttgaatc tatctttgat aaagctggtc
     1081 tctcaatacg ttacgaaatt gtaaagaatg aacgcggtgt aaagtgcatt cacttcttgg
     1141 agtgaaaatt gaattatgag gtgatgaatg attgatttaa cagaacgcta tgaactcatc
     1201 agggcaatta gcgaacgatt gaaagaacaa cccaacaaac caaaacgaat caaatcatcc
     1261 acctctaagc aaaataacag aatcagaaga atcgctaaat ggtgtcttga taaattacca
     1321 agtgattgtc agattgtcgg tgaagatggc aattattatc ttgtcagaca ttaaaaagaa
     1381 ttggttgggt aaaaatcaac gggagagatt atcgagtgac atcggtagct ctcccttttt
     1441 tgtatatgtg ctacgtgatg attcggataa gaattatcct catgaaatct gtaaagctaa
     1501 tacctacact gaaaaataga atttacgtac aacacgctta aagaggtgaa acatgaatga
     1561 gcaaattcac agttgatagt tttatcgttg aactaggctt caatgagaac gttattaaag
     1621 gtctgcaaaa agttgaaaag gcagctttgc agtcagcaca aagaattgaa cgtaatttga
     1681 atagagcgtt taaggtcgat accaagcaac ttgacagtaa tttaacggct tctttaaagt
     1741 caatggaagg gaagattaat aagacctttg accgtctgga agctagagca aaaaatacta
     1801 aagctttccg tttcacaagt caggttaatg acgctattaa tcctccaaaa caaccaagac
     1861 agccacgaat tagcggaaac agagcaatca cagcagctta ttcagctaat atgagcaagc
     1921 tgaaaggtct taacccaatt gtcgagaaat acattaagag tcagttttac gcattaagta
     1981 gtaaagctgg tcagatggat aacaccaagt ttaatgaagc cctcgctaaa ctaaattcaa
     2041 gtgtacgaga agcaattgct aaagccagag gtcatacatc caccagcatt aacggtgata
     2101 gagcagctag cagtcttgat agtctgtctt cagcagcagt taaaacagca ggtgcattta
     2161 tcagctttca ggctgtacta tccgcttatc agcgagtaat ggaagttggc cttcaacgtg
     2221 catcatcgga aagaagtatt gatttcgtat tcggtgacca aagtacacag gttaaaaagt
     2281 tcattcaaga tttaactcaa attactggta tggacacaac cgaaacacaa gcacaatttg
     2341 cttcatttgg tgcatctgca acgactacga tgggtcttga agggtcagaa gagttattta
     2401 aaaaccttac aggttatgct cgtttaatgg gtcgttcaga tgagcagatt caaagagcat
     2461 taaccgcagt gtcacagatg gcctctaagg gtcaggtgat ggctgaggag ttgaaggggc
     2521 aattatctga agctttgccg ggtgctactc aggcatttgc aaaggcttta ggtctgactg
     2581 aaaaacaact tttcgataag atgaaaaatg gtgatgttaa agcctctgat gcacttccta
     2641 agtttgctaa agaacttggt gcacagatta attcaagagg tggctggaaa gctattcagg
     2701 actctacgca gacaatgtta gggaacttaa agaatacctg gaataactca ctaacggata
     2761 ttttcagagg ttctgaaaat ggtctgcaag attttacacg aagcctaaca acgcttttaa
     2821 acagtttagg tggaagtggt aaaagtcttg gtgaatctct tggtagcttg atgactgata
     2881 tgtcgcatgg tgtggatagc ctgaccgata tcagttacag agtgagagct ttctttgatg
     2941 aggtgacatt ggcttaccgt ggtttaaccg atgagcaaaa gaaagtagtt gatgctttag
     3001 gttctggctt gattaatagt ttaaaagtct tggctggtgt tattgctgta aataaagttg
     3061 ttggtgtaac cagaagtatt tacgggctta tgactgctat cacaagatta ggtcaggtcg
     3121 ctgatacaac agcggataac gtgaatagaa agaacgggaa accatcaagg tttaatcttg
     3181 ggaatatgct attagctggt agtgctatct atgcaggtta tgagagatta gcttctacgc
     3241 ctgaacgaat ggaagcacaa gctaaattac cagagctggc taaagagtct ggacttaagc
     3301 cagtatttac tatggacgat gtgaaaggtt ggtttaatag tgctgctatg aatcttgcta
     3361 acttccagca tccagaatta atcaccatga gacaagatgc ggcattaacg aatacggata
     3421 tccaaagcct gagagatgaa attagtgctt tgagcaaacg tattcaggag cctgtgaaag
     3481 tttcacttgg tggtgaagtt gccattaaac cagatgaaac cagctttatg acctttagca
     3541 gtaatatcta cgaccaatac gcagaagcta cgctattaag ttcatcattc ccagaagatg
     3601 attgataata gtcgaatgct acgtgaaatc tcctgaacag atatacccac caaaaattat
     3661 ttttcacgta gctcacgctt aacagataaa acacttaaat atcaaaggct tggttaaaaa
//
LOCUS       DAZFRG010000346         3707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFRG010000346 DAZFRG010000000
VERSION     DAZFRG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.348"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..462
                     /locus_tag="ACHKUC_004718"
     CDS             <1..462
                     /locus_tag="ACHKUC_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6620811.1"
                     /translation="VATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            480..929
                     /locus_tag="ACHKUC_004719"
     CDS             480..929
                     /locus_tag="ACHKUC_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620812.1"
                     /translation="MSAIASTPQTELSWFRYGQYNETGIYISIEKTIIITITVSGGCF
                     KKEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRL
                     RLQDQQEETSVTLFQGKMSTDGNNYSIIKGKVSSVLLLLKMLQINHA"
     gene            1345..2877
                     /locus_tag="ACHKUC_004720"
                     /pseudo
     CDS             1345..2877
                     /locus_tag="ACHKUC_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2911..3420)
                     /locus_tag="ACHKUC_004721"
                     /pseudo
     CDS             complement(2911..3420)
                     /locus_tag="ACHKUC_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            3440..3707
                     /locus_tag="ACHKUC_004722"
                     /pseudo
     CDS             3440..3707
                     /locus_tag="ACHKUC_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1052 a          875 c          891 g          889 t
ORIGIN      
        1 gttgccacac aggagggatg gttgtacctc gctggcatca aagatgttta tacgtgcgaa
       61 attgtcggct acgccatggg agagcgcatg acaaaagagc tgacaggtaa agccctgttt
      121 atggcgctca ggagccagcg cccacctgcc gggctaatcc accactctga tcgaggttca
      181 cagtactgcg catacgatta ccgggtcata caggagcagt ttggtctgaa aacatcaatg
      241 tcgcgtaaag gtaactgtta cgacaacgct ccgatggaaa gcttctgggg aacgctgaaa
      301 aatgagagcc tgagccacta tcgttttaat aaccgggatg aagccatctc agtaatacgg
      361 gaatacattg agattttcta caatcgtcag cgtcgtcact ctcgtctggg gaatatctcc
      421 ccggcagcct tcagggaaaa atatcatcag atggctgctt aaaaaaagaa caaatggttg
      481 tgtccgctat tgccagtaca cctcaaactg agttatcatg gttccgatac ggacagtata
      541 acgaaacggg tatttacatc tcgatcgaga agaccattat cattacaatc accgtttctg
      601 gtgggtgctt taaaaaagaa tacagcattg tctcccacat gctggtcact gacaccataa
      661 cagaggccac cctgtacgaa aacgggctgt atacgcgcca catcagactc agtcgcccag
      721 tatctgacag caaatacccc ttgccacccg gatctcagct cattaaaaat atgaccttac
      781 gactgcgcct gcaggatcag caggaagaaa catccgtgac attatttcag ggaaaaatga
      841 gtaccgacgg aaataactat tccatcatca aaggtaaagt aagctcagta cttctgttat
      901 taaaaatgct gcagattaat catgcctgat ctctgtcccg gtattttagc ccgaggtata
      961 ttctgaaata tcattcatca tgaccggcgt ttatacaaac gccagtcatt cgctaacagc
     1021 ttcaccgttc cttcccccat ccaggttccg catgagcgtg ttgtaatgtt cataagcgta
     1081 ttatcaggat tgtctggaaa attcccgata ctttggacta ttactcctga acaactgtaa
     1141 gcgtacagcc tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa
     1201 ataagtagtg ggaaccaaag tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa
     1261 aggctgccct aattatcctc ccgaatttaa acagcagctc gttgggggat gcggacgatt
     1321 tcctggactc tcaggagata aaacatgttt actgaagaag aaaaaatccg cgccattgag
     1381 ctttatttca aatatggtaa gaagctggct cccgtcgtac gcgaactggg ctacccttcg
     1441 aaaagaaatc tgcggcgttg gatccgctca tgggaagcag gtggtggcgc aaaagaatcc
     1501 attcgtcata agcaccgtta ctccgatgag caaaaacagg ttgccgttga acattatctt
     1561 aaccacggct gctgcctggc gtttaccagc agagccctgg gttatccctg cactgatgta
     1621 ctcgctcgct gggttaatga actttatccc gacagacgac gtatatttac cagtaaggcc
     1681 aacccggttg cgccctttga accagaagtc aaacgtcagg ctgtcatggc actctgtaca
     1741 cgacaagtat cagccagtga gatcgccagg cgtattggtg tcagtcgtgc ggtattgtat
     1801 aaatagaaag atgaaattat cggcaacagt gcttaccaga ctatgcgtaa acataacgaa
     1861 ccttccctgg aggcagaacg cgatgcgttg cgggaggaag tcgcccgact gaatcaggaa
     1921 atacgccgcc ggcagatgga gctggatatt ctgaaaaagg cggaggaaat cataaaaaaa
     1981 gacccgggca tcagtatcag tcacctgaac aacagagaaa aaacaaagat cgctgatgcc
     2041 ctgaggcaaa catatcccct gacagaatta cttcatgttc tgggccttac ccgtagcagt
     2101 tatttttatc accgggctgc actgaaagcc ggtgataaat acgccacgat acgtacgatg
     2161 ctgacagata tatttaacag taattaccag tgttatggct atcgtcgcct gcatgcgatg
     2221 ctcaggcatg agggggggcg gctatcagaa aaggttgtac gcagacttat ggtggaggaa
     2281 cagcttgtcg tcagccgtaa ccgtcgtcgt cgctacagct catattgcgg agaaatcgga
     2341 ccggctccgg ataaccttat cgccagagat tttaaggcgt agcaacctaa tcagaaatgg
     2401 ctgacagata tcacggagtt ccaactccct gcaggtaaag tctggctgtc accggtggtg
     2461 gactgcttcg atggaaaagt tattagctgg tctctcagta cacgccccga tgctgagctg
     2521 gtcaacacta tgctggatag cgctgtcgaa acgttaaatg ctggcgaacg accggtgata
     2581 cacagtgaca gaggtgggca ttatcgctgg ccaggctggc tggaaagagt gaatgcagca
     2641 ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg ataatgccgc atgcgaaggt
     2701 tttttcggca gactgaaaac ggaaatgtat tatgggcgta aatggtcggg catcacgcca
     2761 gaaaagttca tgcagcatgt agatgcttac atcagatggt ataacgagcg gcgtataaaa
     2821 ttatcgctgg gtgcagtcag ccctgaaatg taccgccaac aatgtgggct ggcataataa
     2881 agcagtctag gaaatcgtcc gcatccccaa ttcaggggcg gttttttctt tacggttttt
     2941 cgccataaag actattacta tcagactgac aagactaccc gcgaaagccg ggagttccgc
     3001 gccaagatta acagccacca gatactgggg aattaatgta ctgacaccac acatcagggt
     3061 aattagtcca acgcctcgta tagtcttcat accaccaccg ataatcgcga cgatgacgaa
     3121 aggaagcaga atgttgaaca gtgccagttg aagaataatt gtcccgctga gtgtggttac
     3181 aggtaaatta acttgttcag caagtacaga gacaggtata cctactgccc caaacgctgt
     3241 agggactgta ttagcaacca gtgatgcaat cgctgctttt agtgggttaa agcccagagc
     3301 aatcagtata ccaatgggga ttgctaccgc tgtaccgtat ccggctgccg cctcaagaaa
     3361 tccaccaaaa caccatgaga taagaagtac ctggatacgt ttgtcatcac taacactcgc
     3421 tcaatcattt ggtctgctat ctcaacagat tgctttcaac attattgtca gaatctggag
     3481 cagattgaag atcgcatact tcaactcgat caggaaatat ctcgcctgtt acctgagtga
     3541 tctctgtgca atctggtctg ccagttgcag gctcttaaag gtgttggtca actaatcgca
     3601 ataaccctgg ttgctgaact gggtgatttt tcgcgatttt ccaatcctga acagctcatg
     3661 gcttttctgg gactggtgcc aggtgaatat tccagcggaa acagcat
//
LOCUS       DAZFRG010000347         3630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFRG010000347 DAZFRG010000000
VERSION     DAZFRG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.349"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            248..2182
                     /gene="yeaG"
                     /locus_tag="ACHKUC_004723"
     CDS             248..2182
                     /gene="yeaG"
                     /locus_tag="ACHKUC_004723"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO6620813.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            2295..3578
                     /locus_tag="ACHKUC_004724"
     CDS             2295..3578
                     /locus_tag="ACHKUC_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO6620814.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQDGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT          983 a          873 c          911 g          863 t
ORIGIN      
        1 aaatttatgg atgagttgtt gatatgccat tgaaattaag aaagccgtgc aggcaagttt
       61 tccatttgcc atctacgctt aattttgaag gtgtatcacc gggcacgttg ttctcatcgt
      121 caataaaatg gcatgagagt tgctgtgttt tagcaagaga cgtcgttcag tttacctctt
      181 ccgggagcct caactattca tatgaacggc tcttaacctg tgctaaaaaa cgaaaggacg
      241 gcataccatg aatatattcg atcactatcg ccagcgatat gaagctgcca aggacgaaga
      301 gttcacactg caggagtttc ttaccacttg tcggcaagat cgcagtgctt atgccaacgc
      361 ggctgagcgg ctattgatgg ctattggtga gcctgtcatg gtcgatacag cccaggaacc
      421 cagactttct cgactctttt ctaaccgggt cattgcacgt tatccggcgt ttgaagagtt
      481 ttacggcatg gaagacgcga ttgaacagat tgtatcttat ctgaaacacg cggctcaggg
      541 gctggaagag aagaaacaaa tcctgtatct gctggggcct gtgggtgggg gtaaatcatc
      601 gcttgctgag cgactgaaat cattaatgca gctcgtaccg atttatgtat tgagcgccaa
      661 cggtgagcgt agcccggtca acgatcatcc gttctgtctt ttcaatccgc aggaagatgc
      721 gcagattctg gaaaaagagt atggcattcc tcgccgttat ctcggcacca tcatgtcgcc
      781 gtgggcggca aaacgcctgc atgaatttgg tggcgatatc actaagttcc gggtagtgaa
      841 ggtctggccg tcaattctgc aacaaattgc tatcgccaaa acagaacccg gcgatgagaa
      901 caaccaggac atctccgccc tggtagggaa agtcgatatt cgtaaactcg aacactacgc
      961 gcagaatgac ccggacgcct acggctattc cggtgcgctg tgccgcgcca atcaggggat
     1021 catggaattt gtcgagatgt ttaaagcacc gataaaagtg ctgcatccct tgttaaccgc
     1081 cacccaggaa ggtaactaca acgggacgga aggtatctcc gccctgccgt tcaacgggat
     1141 tattctcgcc cactcgaacg aatccgaatg ggtcactttc cgtaataaca aaaacaacga
     1201 agccttcctc gaccgtgttt acatcgtgaa ggtgccgtat tgcttgcgca tttccgaaga
     1261 gatcaaaatc tacgagaaat tgcttaatca cagtgaattg actcacgccc cctgcgcccc
     1321 tggcacgctg gaaacgctgt cacgtttttc tattctttcg cgtctgaaag agccagaaaa
     1381 ctccagcatt tattcaaaga tgcgggttta tgatggtgaa agtttgaaag acactgatcc
     1441 caaagccaag tcgtatcagg aatatcgtga ctacgctggt gtcgatgaag ggatgaacgg
     1501 tctgtcgacg cgttttgcgt ttaagatcct ctcccgcgtg ttcaacttcg atcatgtaga
     1561 agtggcggca aacccggtcc atctgttcta cgtcctggaa cagcagatcg agcgcgagca
     1621 gttcccgcaa gagcaggcag aacgctatct ggagttcctg aaaggttatc tgatcccgaa
     1681 atatgccgag tttatcggca aagagatcca gacggcctac cttgaatcct attcagaata
     1741 tgggcagaac atttttgacc gttatgttac ctacgcggat ttctggattc aggatcagga
     1801 gtatcgcgat ccggataccg ggcagttgtt tgaccgcgag tctcttaacg ccgagctgga
     1861 gaaaatcgag aaaccggcgg ggatcagcaa tccaaaagat ttccgcaacg agattgttaa
     1921 cttcgtactg cgagccagag ccaataacag cggacgtaat ccgaactgga ccagctatga
     1981 aaaactgcgc acggttattg agaagaaaat gttctctaat accgaggagc tgttgccggt
     2041 tatttcgttt aacgccaaaa cgtcaaccga cgagcagaag aaacacgacg actttgtcga
     2101 ccgtatgatg gaaaaaggct acacccgtaa acaggtgcgt ttactgtgcg aatggtattt
     2161 gcgcgtacgt aaatcgtctt aacaaccctg gcccggtaca catgctaccg ggcctacaac
     2221 gacagcgaac cgtgggccag agaagcggca acacaggcgt agcataccgt tggcaaatgt
     2281 agtacggggg gcatatgacc tggtttattg accggcgtct gaacggcaaa aataaaagca
     2341 tggtgaatcg ccagcgtttc ttacgccgtt ataaagcgca aattaaacag tcgatctccg
     2401 aggccataaa taagcgttcg gtgactgacg tcgacagcgg cgagtccgta tccattccca
     2461 cggaagatat tagcgaaccg atgtttcatc aggggcgtgg tggcctgcgc caccgcgtac
     2521 atccgggcaa tgaccatttc gtccagaacg accgaattga acgtcctcag gatggtggcg
     2581 gaggttccgg cagtggtcag ggccaggcca gccaggatgg tgaaggtcag gatgaatttg
     2641 tctttcagat ttcgaaagat gagtatcttg atctgctctt tgaagatttg gccttaccaa
     2701 atctgaaaca aaaccaacag cgccagttaa ccgaatataa aacgcatcgg gcaggttata
     2761 ccgctaacgg cgttccagcc aatatcagcg ttgtacgttc attgcagaac tcactggcgc
     2821 ggcgcacagc catgacggcg ggcaagcggc gggaacttca tgcactggaa gagaatttgg
     2881 ccatcatcag caacagtgaa cctgcgcaac tgctggaaga ggaacggctg cgcaaagaga
     2941 ttgctgaatt gcgcgccaaa attgaacgcg ttcctttcat tgacactttc gatttacgtt
     3001 acaagaacta cgaaaaacgg cccgatccct ccagccaggc agtgatgttt tgcctgatgg
     3061 acgtttccgg ttcaatggat cagtccacta aagatatggc taaacgtttt tatattctgc
     3121 tgtatctgtt cctcagcaga acgtataaga acgtggaagt cgtatacatc cgccatcata
     3181 cccaggcgaa agaagtcgat gaacatgagt ttttctactc gcaggaaact ggcggcacca
     3241 ttgtttccag cgccctaaaa ctgatggatg aggtagtgaa agagcgttat aacccggcac
     3301 agtggaatat ttacgcagcc caagcatcag acggcgataa ctgggccgat gactctccgc
     3361 tttgccatga aattctggcg aaaaaaatat tacccgttgt tcgttattac agctatatcg
     3421 aaattacacg tcgcgcgcat cagacactgt ggcgagaata tgagcatctg caatctactt
     3481 tcgacaactt tgcgatgcag catatccgcg accaggatga tatttatccg gtgttccgtg
     3541 aactgtttca taaacaaaat gcaacagcta aagactaaaa ctgtcagccg ggtcattatc
     3601 gcctggctga tttttgtgca cagaaaaccc
//
LOCUS       DAZFRG010000348         3597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFRG010000348 DAZFRG010000000
VERSION     DAZFRG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.350"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..1004
                     /locus_tag="ACHKUC_004725"
     CDS             150..1004
                     /locus_tag="ACHKUC_004725"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO6620815.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCICRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1040..1930
                     /locus_tag="ACHKUC_004726"
     CDS             1040..1930
                     /locus_tag="ACHKUC_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6620816.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            1991..3490
                     /locus_tag="ACHKUC_004727"
     CDS             1991..3490
                     /locus_tag="ACHKUC_004727"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6620817.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKTDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3491..>3597
                     /locus_tag="ACHKUC_004728"
     CDS             3491..>3597
                     /locus_tag="ACHKUC_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6620818.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVV"
BASE COUNT         1012 a          719 c          896 g          970 t
ORIGIN      
        1 aattaagtta ttttttgtaa gttattttga tgatgttcga catgcatcac gctttttgct
       61 gcttttgctg tgctagtttg ctttcaaaat gaaagtgaag attctctttc ttcattttgt
      121 aaggcgaacg acaaataaca ggagctgcga tgtatattat ttcaagcaaa aacatgctga
      181 aaaaagccca gtcagaggga tatgccgtcc ctgctttcaa cattcataac cttgaaactt
      241 tacaggtagt tgttgagagc gctgcacaac ttcgctctcc cgttatgctg gcgggaacac
      301 ccggtacata tcgttatggt ggcgtgggaa gtttaatttc gttagtacaa tcgctggctc
      361 gcgagtacaa cctgccgtta gtcctgcatc tcgaccatca tgaagagagt gatgacattt
      421 ttaacaaggt tcgcgcaggg atccgttctg tcatgattga tggctcccat ttgccatttg
      481 aagacaacat tgcgcgagtg caagaagtta ccaccttttg ccatcgcttt gatgttagcg
      541 ttgaggccga gctagggcgt cttggcggcc aggaggacga tctgcgggtg gatgaaaaag
      601 attccgctta tacctcacca gccgcagctg cagagtttgt tcaacgtacc caaattgact
      661 ccctggcggt agcaatcggc acagcacatg gtttatatgc gcatgaacca cgactggatt
      721 ttgtgcggct ggaacagatt cgtcagcgag tggagattcc gctggtgttg catggtgctt
      781 cgggcttatc ggaacaggac gttcagcact gtatttgtcg cggtatttgt aaagtgaatg
      841 tcgcgacaga attgaaaatt gcgtttgccg atgcgttaaa aggatatttc tacaacaatc
      901 ctgcggcaaa tgatccacgt cactatatgc agcccgcaaa ggccgccatg aaagaagtcg
      961 taactcgtat tattggcgtc tgtggtagtg aaggaaaaat ttaagacgtt gtaccctaca
     1021 aaaataatga ggattgttta tgaaaaatat taaattactt ggttcagcta tgttggtgag
     1081 cctggcattg ttctcgcaaa gttcgttggc aaaagagtat aaaattggtg cgtcattatt
     1141 aacacaacaa catcctttct atattgatct tgcgaatgcc atgaaagaag aggcaaaaaa
     1201 agacaatgtc aatttaagtg tctctattgc caatcaggat ttaaacaaac aactttctga
     1261 tgttgaggat tttattacta aaaaagtgga tgccattatt atctctccgg ttgattcgaa
     1321 aggcgtccag gcagcgataa ttaaagccga taaggccggt attcccgtta tcaccgttga
     1381 cgtcgctgcg gaaggcgttc cggtcgtatc gcatgttgcg accgataact atgcgggagg
     1441 cgttgaagca ggcaagttaa tgggtaaact tttgcatggt aaaggtactg tagccattat
     1501 ttcttatccg gcactacaat cggtggttgc gcgcgttgat ggttttaaga aagggttaag
     1561 cgatacaccg gatattaaaa ttgttgctga acagcctggt attacgcggg ctgaagcgtt
     1621 aacgactgcg caaaacatta tgcaggcaaa cgccaacctg aatggtctgt ttggctttgg
     1681 tgatgatgcg gcgctggcag cggttatcgc agcaaaatct gcgcataacg acaacatcaa
     1741 aatcattggt tttgacggca tgaaagaagc gcgtgatgcc gttgacagtg aaaaaacgtt
     1801 tgcggccgtg attcggcaat atcccgatca aatgggggct aaagccattg atgccgctgt
     1861 tgatcacctc aatggtaaac cggtagaaaa aatgatcccg gtacaacccg gcgtttatac
     1921 aggtaaatag taaatatctt cacctcacaa caaaacgtta ttggcgtttt gttgtgacac
     1981 cgaggtcatt atgacggatg ttattcttga tgtttcgcat attgcgaaga catttggtca
     2041 tgttcaggca ttaaaagata ttacactctc tttacgtaaa gggcgggtgc atactttatt
     2101 aggcgagaat ggtgccggta aatcgacatt aatgaaaata ctggccggtg tttatccgcc
     2161 aacccaggga acgataacac tacgtggtga aaccatcacc attaataatc cgcagcattc
     2221 tcgccagtta gggattgcga ttatttttca ggaactgagc ctgagtaata atatgaccgt
     2281 ggcggaaaat atttatgcca ataatgaacc gcgacgtttt ggcatcatta acgataaaaa
     2341 aatgctcgca gattgccaga acctcctcgc ggaactgggc atcccactag accctctgga
     2401 aatggtcggc aatatgtcga tggcacatag gcaactggta gaaattgcca aagccttaag
     2461 ctatgcagcg gatgtcgtga ttatggatga accgacatcc tcgctaagtg ataatgaagc
     2521 ggagattctg tttaacatta ttgaaaaatt aaagcagcga ggctgtgcgg ttatttatat
     2581 ctctcatcgc atggatgaaa ttatgcggat ttcagatgat atttcggtga tccgcgacgg
     2641 tgagtatatc gctacgcatg aaaagaaaaa cagtgatatc cagcacttga ttgcccaaat
     2701 ggttggtcgg gaaatgaaaa atatttggcc agcacgtctt ggggaaaaaa cggatgaaaa
     2761 tgttcctgca aaactggaag taaaaaatct ttcccatcct tcgcttttta aagaggtcag
     2821 tttcgctgtg cgtccggggg aagtcctcgg attttttggc ctggtggggg ccgggcgttc
     2881 cgatgtgatg aaggcgctat ttggcctcgt ttcgtatcac gggactgtgt tgattgatgg
     2941 taaggaagtc aggatcgcta atcccaaaca ggctattgat catggcattg cttttgtgac
     3001 cgaaaaccgc aaggaagaag ggttagtgct gatgcacgac gtcaatatga acacgcacca
     3061 tgtggcgttt caatataacg cttcgcggat gggactcatc aatcaccgac aagaagaggc
     3121 gaagacgctg caatcaattg ccagaatgaa cactaaagtg agttctgtgc atcaggcagt
     3181 gggagcactg agcggcggta atcaacagaa aatcgtgtta tcgaagtggc tggagaaaac
     3241 tccgcgtatt ttattgctcg atgagccaac gcgcggagtg gatgtcggcg caaaatttga
     3301 aatctataac gtgatacgtc aactggctgc ggcagggaca gcaattattc tggtgtcctc
     3361 tgaactgcca gaagtcatgg cacttagcga tcgtctggtg gtaatgcgta ataaaacgat
     3421 tgccgatatt tattcatgtg aaaatttaac gcagacacag gtaatgaccg ctgcaacagg
     3481 agtacgttaa atgacagaga aaactcttgc cagtccgaaa ccttcggaaa ttcccattgc
     3541 aaataatttt aaaaaagtct tcaggcaata tggtggtatc ctcagtggaa tggtggt
//
LOCUS       DAZFRG010000349         3597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFRG010000349 DAZFRG010000000
VERSION     DAZFRG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.351"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..747
                     /locus_tag="ACHKUC_004729"
     CDS             <1..747
                     /locus_tag="ACHKUC_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO6620819.1"
                     /translation="RLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNSGFVNT
                     HRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSDMTFFG
                     KIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQTNGWNG
                     YVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWKFYPRW
                     KASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            767..2260
                     /locus_tag="ACHKUC_004730"
     CDS             767..2260
                     /locus_tag="ACHKUC_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6620820.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLANTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAMGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            2389..>3597
                     /locus_tag="ACHKUC_004731"
     CDS             2389..>3597
                     /locus_tag="ACHKUC_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO6620821.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSSSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTL"
BASE COUNT         1024 a          723 c          842 g         1008 t
ORIGIN      
        1 cgacttctta ttgcgtgtac cttgataaca gctatctatc attttcctgc atattcttca
       61 ttagaatata aaggaacctt tggttcaata aatgcgggtt atgcagactg gaatagtgga
      121 tttgtaaaca ctcaccgtgg tgaagtatgg aaagtgactg cggattttgg ggtaaatttt
      181 aaagaagcag aattttactc attttatgaa agtaatgtac tcaatcatgc tgtagcaggg
      241 agaaatcata cggtttcagc aatgacgcat gtcagactct ttgactctga tatgacattc
      301 tttggcaaaa tttatggcca atgggataac tcatggggtg acgatctgga catgttttat
      361 ggattcggtt acctcggctg gaacggcgag tggggctttt ttaaaccgta tattggattg
      421 cataatcaat ctggtgacta cgtatcagct aaatatggtc aaacgaatgg ttggaatggt
      481 tatgttgttg gctggacagc agtattacca tttacgttat ttgacgaaaa atttgtttta
      541 tctaactgga atgaaataga actggacagg aacgatgctt acacggagca gcaatttggc
      601 cggaacgggt taaatggcgg tttaactatt gcctggaagt tctatcctcg ctggaaagca
      661 agtgtgacgt ggcgttattt cgataataag ctgggctacg atggctttgg cgatcaaatg
      721 atttatatgc ttggttatga tttctaattt ttatcaggga ataattatgc agaaaacgtt
      781 aatggccagt ttgatcggcc ttgctgtttg cacagggaat gcttttagtc ctgccttagc
      841 cgcagaggct aaacaaccta atttagtcat tattatggcg gatgatttag gttatggcga
      901 tttagcaaca tatggtcatc agatcgttaa aacacctaat atcgacaggc ttgcccagga
      961 aggggtcaaa tttactgact actatgcccc cgctccttta agttcacctt cacgcgcagg
     1021 gctattaacc ggccggatgc catttcgtac tggaattcgc tcatggattc cttcaggcaa
     1081 agatgttgcc ttagggcgta acgaactcac gattgctaat ctactcaaag cgcaagggta
     1141 cgacacggca atgatgggta agctgcatct gaatgcaggc ggcgatcgca ccgatcagcc
     1201 acaagcacaa gatatgggct ttgattactc actggctaat acggcgggct ttgttaccga
     1261 cgccacgctg gataacgcta aagaacgccc gcgttatggc atggtttacc cgacaggctg
     1321 gctacgtaat gggcaaccca ctccacgagc cgataaaatg agcggtgagt atgtcagttc
     1381 ggaagtcgtc aactggctgg ataacaaaaa ggacagcaag cctttcttcc tctatgttgc
     1441 ttttaccgaa gtgcatagcc ccctggcttc gcccaaaaaa tacctcgaca tgtactcaca
     1501 atatatgagc gcgtatcaga agcagcatcc tgatttattt tatggcgact gggcagacaa
     1561 accctggcgt ggtgtggggg aatattatgc caatatcagc tatctggatg cacaggttgg
     1621 aaaagtgctg gataaaatca aagcgatggg tgaagaagat aacacaatcg ttatttttac
     1681 cagtgataac ggtccggtaa cgcgtgaagc gcgcaaagtg tatgagctga atttggcagg
     1741 ggaaacggat ggattacgcg gtcgcaagga taacctttgg gaaggcggaa ttcgtgttcc
     1801 agccattatt aaatatggta aacatctacc acagggaatg gtttcagata cacccgttta
     1861 tggtctggac tggatgccta ctttagcgaa aatgatgaac ttcaaattac ctacagaccg
     1921 tactttcgat ggtgaatcgc tggttcctgt tcttgagcaa aaagcattga aacgcgaaaa
     1981 gccattaatt ttcgggattg atatgccatt ccaggatgat ccaaccgatg aatgggcgat
     2041 ccgtgatggt gactggaaga tgattatcga tcgcaataat aaaccgaaat atctctacaa
     2101 tctgaaatct gatcgttatg aaacacttaa tctgatcggt aaaaaaccag atattgaaaa
     2161 acagatgtat ggtaagtttt taaaatataa aactgatatt gataatgatt ctctaatgaa
     2221 agccagaggt gataaaccag aagcggtgac ctggggctaa cctgataatc ataataaatg
     2281 taatgtatgc catttagggc tatgcccgcc tgattcagtg ctacgcattg aatcaggttc
     2341 ttttcgcgtt gaacaatatc aagatacggc tattcgcagg gagtggatat gtttaccaac
     2401 gtaaacgttg attgttgcaa aacaccagga tgtaaaaacc tggggttgct gaatagccag
     2461 gattatgtcg cacagggtaa aaatatttta tgccgtgaat gtggttactt gtttccagtg
     2521 atatctgaac agtcgcttaa tatttatcgt aatattgtga atcactcctg gagaggtttg
     2581 atttgccaat gttcaacttg cggaggcacg tccctcaaaa aatatggata ttctgcacaa
     2641 ggccagagaa gaatgtattg ccatcattgt gagaaaacat ttatcactct ggaacatgta
     2701 attaccacac cacgaggagc ccagttagca ttgatgattg agcaagggga ggcacttgcg
     2761 gatatcagaa agtcattact tcttaacagc actggactta gccgtgaact gttaaaatta
     2821 gcgcgtgaag caaactataa agaaagtcga cagtgtttcc cagcttctga tattaccctg
     2881 agtacccgcg cttttcgcgt caagtataat ggtagcaata actctcttta tgctcttgtt
     2941 accgcagaag aacaaagcgg cagggtggtt gccatctcaa ccaattactc ctcatctgcc
     3001 gtagagcaac attatcaata cacatcgaac tatgaagagc gtatgtctcc agggacgctg
     3061 gcacatcatg tccagcgcaa agagttactt actatgcggc gggatacctt gtttgatatt
     3121 gattacggcc cggcagtttt acatcaaaac gatccgggaa tgttggtaaa accggttctt
     3181 ccggcatatc gtcattttga actggtcaga atactgaccg atgagcattc caacaacgtt
     3241 cagcattacc ttgatcacga atgctttata ttgggcggct gcctgatggc taatttgcag
     3301 catattcatc aaggtcgctg ccatatttcc tttgtcaaag agcgcggtgt ggcacccgcc
     3361 accattgatt ttccaccgcg attattcctt agtggtgggg tacgaaataa tgtctggcgt
     3421 gcattttcta accgcaatta ttcaatggct gtatgcaatc tcactggcag taagaaagtc
     3481 cgcgagatgc ggcatgcaac attgaacagt gcgacgcgtt ttatccactt tgtggagaac
     3541 catcctttcc ttatatcatt gaaccgaatg tctcctgcga atgtcgtttc tacatta
//
LOCUS       DAZFRG010000350         3590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFRG010000350 DAZFRG010000000
VERSION     DAZFRG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.352"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..542)
                     /gene="luxS"
                     /locus_tag="ACHKUC_004732"
     CDS             complement(27..542)
                     /gene="luxS"
                     /locus_tag="ACHKUC_004732"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO6620822.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(692..2248)
                     /gene="gshA"
                     /locus_tag="ACHKUC_004733"
     CDS             complement(692..2248)
                     /gene="gshA"
                     /locus_tag="ACHKUC_004733"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO6620823.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKKKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(2321..2749)
                     /locus_tag="ACHKUC_004734"
     CDS             complement(2321..2749)
                     /locus_tag="ACHKUC_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO6620824.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(2746..3312)
                     /gene="yqaB"
                     /locus_tag="ACHKUC_004735"
     CDS             complement(2746..3312)
                     /gene="yqaB"
                     /locus_tag="ACHKUC_004735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO6620825.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          899 a          967 c          840 g          884 t
ORIGIN      
        1 aattaattgt gaagatagtt tactgactaa atgtgcagtt cctgcaactt ctctttcggc
       61 agcgccagtt cttcgttgct gttgatgcgc acgtcacgtt ccagaatgtt acgcgcaata
      121 tcctgcgctt cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc
      181 gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca
      241 gcgacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc
      301 attggcgaga tatcgataat ctctacgcca ttaccgttaa gatggttacg cataaaacca
      361 gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg
      421 cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgtttttgcc
      481 acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc
      541 atttagccac ctccggtaat ttttttaaaa attttctgaa ctctttgttc ccaggcgagt
      601 ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt
      661 ggccactcac gagtggcctt tttcttttct gtcaggcgtg cttttccagc cacaccgcaa
      721 acggttcggt atcagcggct tccatttcct gctgacggcg ttcagacgcc tcgcgctcgg
      781 cgacaaaatc ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtacg
      841 cttcagcaaa cgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac
      901 gggcagagaa agtcagatca ggattatcga agcaggcgac cagttcatca cacactttct
      961 gatacgcttc gccgccgtta atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga
     1021 acagatcttt acccacctgc ggtaacggga actgcgcagt ttcgcagccg atacccagcg
     1081 tcaggcccgg tttgcgacct tcgaggatca cccggttcca gttaacgcgt gtacaggcaa
     1141 gttcgctact gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga
     1201 ggaatcgcac ctgttgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgagc
     1261 ggacttcaat atattcaatg ccgccacgta acagcgcatc agaaggcgac tcgccgctgc
     1321 gggtaacgcg ttttggacga atcggcgcgt acagttcgtt ttcaatctgc agcacgttgc
     1381 tgttgatttg cagccgctta ccgtctttct caataccaat cttcgcgtac tcttccgatg
     1441 gcgttttgat tgcctgttta aggcccgcca cgtactcgta gagatcattg aaggtgatac
     1501 caagattgct ttgcgattta ttggtatagc caagatcgct caaacgaaga gaggtcgcat
     1561 acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt ggttttcctt
     1621 gcaggaaaga agaacaaatc gctggagacg caccaaacag ataaggaatg acccaaccga
     1681 aacgatagta attgcggata acgcggaaat agcccgcaga aattttcttt ttggcatcag
     1741 cgcccgagat atcaccgcac ttcgcttgcc agaatgccat tggcaaagag aaattatagt
     1801 gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacggtaca
     1861 gcgttttaaa gcgaccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt
     1921 ctgcgatata gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg
     1981 tatagcgatg cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg
     2041 taataaattc cagcaatgct tccgcaaaat cggtagtaat ccatttatgg gtcagtgcag
     2101 aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag
     2161 tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg
     2221 ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat
     2281 tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc
     2341 cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa
     2401 agattaccgg tccccacgag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa
     2461 ccacgggcat ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag
     2521 ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag
     2581 ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacccag ggatgactca
     2641 ctccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc
     2701 tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac
     2761 atccacggcg tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa
     2821 gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg
     2881 cgcgggttta tggtgtttga cgtgatcggc agcgacgacg gcgtcaaaat aacggcgtaa
     2941 tcccaggtgt gccagcaatg cctcagcgat ggcgctttca ctccccgttc ctacagccat
     3001 tgggcgacga ccatgccagc ttttcaccac ttcaacaaga ggaagcggct cgacactatc
     3061 cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc
     3121 ggcctgattc agctcaataa tcgcctgagc aatacgccag gtgggcgagc cattaagcgc
     3181 aatcatcgcc tgaacatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt
     3241 acggtgcgta ggctcggtat ccaggatggt gccatccata tcaaaaatta aacctgcata
     3301 acgctcgtac atggtcttct cgcaagtaga aaaatatgac gctactttag cgtaattgct
     3361 tgattttgtc gctgatggag aaggagaaga aaattcagga aatggataaa gtagttattt
     3421 agaaatgaga tatttttgaa ggaaattttt ttgagaagat ggtgcatccg ggaggatgac
     3481 tcactggcgt tcgcccttcg ggccgctgct ggcgcaacgt tatcctccct ggtgcctgcg
     3541 gttaataccg ctacctcttc gataccttca ttgctgaagg ttacggagaa
//
LOCUS       DAZFRG010000351         3555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFRG010000351 DAZFRG010000000
VERSION     DAZFRG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.353"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(140..775)
                     /gene="msrQ"
                     /locus_tag="ACHKUC_004736"
     CDS             complement(140..775)
                     /gene="msrQ"
                     /locus_tag="ACHKUC_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO6620826.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(776..1780)
                     /gene="msrP"
                     /locus_tag="ACHKUC_004737"
     CDS             complement(776..1780)
                     /gene="msrP"
                     /locus_tag="ACHKUC_004737"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO6620827.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAV
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1889..2302)
                     /gene="hiuH"
                     /locus_tag="ACHKUC_004738"
     CDS             complement(1889..2302)
                     /gene="hiuH"
                     /locus_tag="ACHKUC_004738"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO6620828.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2435..3106
                     /gene="hprR"
                     /locus_tag="ACHKUC_004739"
     CDS             2435..3106
                     /gene="hprR"
                     /locus_tag="ACHKUC_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO6620829.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3106..>3555
                     /gene="hprS"
                     /locus_tag="ACHKUC_004740"
     CDS             3106..>3555
                     /gene="hprS"
                     /locus_tag="ACHKUC_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6620830.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDAFRINIDEVSPSLTVTVAKLASAR"
BASE COUNT          971 a          774 c          862 g          948 t
ORIGIN      
        1 tattgaaata aaataaatta ttctggtgat gaaaggttga tgacaaacga aacataactt
       61 gatgcgatct gtgtgggccg gatagggcgc tcgcgccgca tccggccggg ataacgcgaa
      121 gtttgtcggc aatatgaagt tatagagata atttgttgtg ggcctgcttg cgtaaccggt
      181 tgaaaaggga aagcaacttc ttataccgta aggctaaaag cagtacagcc agcccagcgt
      241 agatgagtgg ctgcggtgaa ataatcttca ctgaccacag ataatgtatt ggggccagga
      301 tcgcgacaag atagacgaag ttatgcaact gttgccagtg tttgccgagt tttcgctgca
      361 tcgactgggt tgaagtgaac gctaaagcaa gcaaaataac ccagctgata attcctaacg
      421 ttaaataagg ccgggtaatt aactccttac ctaataacgc cagattattc acgcccaact
      481 ccagcaatgc gtaactggtt aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca
      541 ggcggcgagt gcgtatcaat aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca
      601 aggccgcaag caaaaatttc agtgcagtgc gaccggtaaa atgctgaata tctttcaccg
      661 gatcggcacc tagtccaccg tgattgatcg cccagaccag ccagagaaac ggcaacaatc
      721 cggcaagatg caggcaaact ttcagccata tcacctgctt tgctgtcaga cgcatttaga
      781 aattctcccg caaatccagg ccacgataca gcgatgccac ctggtcggcg taaccattaa
      841 acagtagtgt tggctggcgc tgaacatcga gaatgccgcc tgaaccaata aatcgttcgg
      901 tagcctgtga ccagcgcggg tgatcaacat gcggattaac gttggcgtaa aaaccgtatt
      961 cgtcaggcgc tgccagattc caggtggttg gcggacgctc gcgggtcagc ttaatactga
     1021 cgatcgattt aatcccttta aagccatatt tccacggcac aatcagtcgc accggtgcgc
     1081 cattttgcgg tggtaacgcc ttgccataaa cacctacggt catcagtgtg agcggatgca
     1141 ttgcttcgtc gagacgcaat ccttcgacat aaggatattt cagcccgccg ccgataaagc
     1201 ggtcctgctg gcctggcatc tgttccggcg cataaatcgt ttcgaaagcg acatacttcg
     1261 cattgctggt gggttcggca agcgccagca atttgtgtag cggaaaacca atccacggca
     1321 ccaccatcga ccacgcttcc acgcagcgca tacgataaat acgctcttcc agcgggaagc
     1381 gacgggttaa atcatcatga tcgagggtca atggttttgc cacttcgccg ctgattttca
     1441 gtgtccatgg atcggttttc aggctaccag cattaaccgc gggatcggct ttatccagcc
     1501 cgaattcata gaagttgtta taaccggaga ctttatctgc tggcgtcagt ggcaggttat
     1561 tttgccaggc ggcaggcttg ctgaactcca gcggttttcc ggcgggggcg ggcggacgat
     1621 cgttcccttt aaaccagcta agcagatcgg catgcgcagc gtgaggcaaa gaaaatgcag
     1681 ctgcgctgat gcccagtgct tttaacacct gccgacgctt cataaagaat accgactcgg
     1741 ccgtaacatc tgattctttt aaaaattgat tctttttcat aagccatcac acttttttgt
     1801 cgttagccaa caagagccgt ttactgttaa tctgtcaggg agtcacctcc ctaacagtag
     1861 cagagatcag cttttacata tttacgcatt aactgccacg ataggttgaa tacccatatt
     1921 ggctgagtaa taaaggcaca tgataatgct cgttcacttt attaatatga aattcaacgg
     1981 ggatctcagg gaagaaactt tcaagatttt gtttcttgaa atagtccccg gttttaaata
     2041 cgacacggta atcgccagta gtcgcagttt gctcgggcca cagtgcctta attcgtccat
     2101 ccttatctgt tttggcggta ttaagttgta accagccgtt atccgctttc ttttcaagag
     2161 tgactgtcac gtcggcagcg ggttttcctg tttgctggtt caaaatgtgc acgctaagaa
     2221 tgttttgttg tgcggcataa accaaagaag gtaatgaaaa tgctgccgtt gctaccgaga
     2281 gtactaaata acgctttaac atgtttatat ccttgtcatg tgaatgagtt ttgaggatag
     2341 cgaaaaaacg tccagggtaa ccttacagca gcattacaat tttgtaatga accaggttgt
     2401 ttctataaca tatgatttat ggcatattat tttcatgaag attctactta ttgaagataa
     2461 tcaaaggacc caggaatggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc
     2521 cgtttctgat ggcagagatg ggctttatct tgcgctgaag gatgattatg cattgatcat
     2581 tctggatatt atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacggc
     2641 aaagcaaact cctgttattt gccttacagc aagggattct gtcgatgaca gagtcagagg
     2701 gctggacagt ggagcaaatg attatctggt aaaacctttt tcattttctg agttgctggc
     2761 aagggttcgg gcacaattaa ggcaacatca cgctctgaat tcaacattag aaatcagtgg
     2821 cttaaaaatg gactctgtca gtcaaagtgt gagcagggac aatatcagta ttacactgac
     2881 gcgcaaggag tttcagttac tttggctact ggcctccaga gctggcgaga ttatacccag
     2941 aacggttatt gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga
     3001 tgtcgccatt cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctaatcgc
     3061 cacaatccgg gggatgggct attcattcgt agcggtaaaa aaataatgaa aagattatct
     3121 ataaccgtcc gtttaacctt gctttttata tttctgctgt ctgttgctgg cgctggaatt
     3181 gtatggactc tctataatgg cctggcaagt gagttgaaat ggcgcgatga tacaacactc
     3241 attaaccgga cagcgcagat caagcagttg ttaattgatg gggtaaatcc agatacgtta
     3301 cctgtgtact ttaaccggat gatggatgtt agtcaggata ttttgatcat tcatggtgat
     3361 ggcatcaata aaattgttaa ccggacaaat gtcagtgatg acatgttaaa taacatacct
     3421 gctagtgaga caatcagcgc agcaggcatt tacagaagca ttattaatga tacagagata
     3481 gatgctttcc gaattaatat tgatgaagtt tcgccatcat taacggttac tgtggctaaa
     3541 ttggcttcag ccaga
//
LOCUS       DAZFRG010000352         3548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFRG010000352 DAZFRG010000000
VERSION     DAZFRG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.354"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1088
                     /gene="adrA"
                     /locus_tag="ACHKUC_004741"
                     /gene_synonym="dgcC"
     CDS             <1..1088
                     /gene="adrA"
                     /locus_tag="ACHKUC_004741"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO6620831.1"
                     /translation="FFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGLAGMFL
                     PIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAVLAGMW
                     VGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSA
                     PLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETML
                     RNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIG
                     RFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVGVAPLNPQMSH
                     YREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1105..1914)
                     /gene="proC"
                     /locus_tag="ACHKUC_004742"
     CDS             complement(1105..1914)
                     /gene="proC"
                     /locus_tag="ACHKUC_004742"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO6620832.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHSVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2034..2492
                     /locus_tag="ACHKUC_004743"
     CDS             2034..2492
                     /locus_tag="ACHKUC_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO6620833.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2675..3199
                     /gene="aroL"
                     /locus_tag="ACHKUC_004744"
     CDS             2675..3199
                     /gene="aroL"
                     /locus_tag="ACHKUC_004744"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO6620834.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDLWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            3249..3440
                     /gene="yaiA"
                     /locus_tag="ACHKUC_004745"
     CDS             3249..3440
                     /gene="yaiA"
                     /locus_tag="ACHKUC_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO6620835.1"
                     /translation="MPTKPPYPREAYIVTIDKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          845 a          875 c          969 g          859 t
ORIGIN      
        1 actttttcaa aaaagcggcg gcgcatgggg aggaacctcc tttaactcct caaaacgaac
       61 atcagcggtc cgggctgcgc tttgctcgtc gcgtcagact accccgcgcg gttggcctgg
      121 ctggcatgtt cttaccgatt gcttcaacgc tggtttcgca tccgtcgccg ggctggtggt
      181 ggctgttgtt ggtcggctgg gcgttcgtct ggccgcattt agcctggcaa atagcgagca
      241 gggccgtcga tccgcttagc cgggaaattt acaacttaaa aaccgatgca gtattagcgg
      301 gaatgtgggt aggcgtaatg ggcgtaaacg cactgccttc caccgcgatg ttgatgatta
      361 tgtgtctgaa tttgatgggg gcaggcggcc cccgtctgtt tgtcgcgggt ctggtgttga
      421 tggtggtctc ctgccttgtc accctcgagc tgacgggcat caccgtgtcg ttcaatagcg
      481 caccgctgga atggtggctc tcccttccca ttattgtcat ttatcctctg ctgtttggct
      541 gggtcagcta ccagacggcg actaaactgg cggaacataa acgcaggttg caggtcatga
      601 gtacccgcga cggtatgacg ggcgtgtata accgacgtca ttgggaaact atgttacgca
      661 atgaatttga taactgtcgg cggcataatc gcgatgcaac gttactgatt atcgatatcg
      721 accatttcaa gagcatcaac gatacctggg gccatgatgt gggtgatgaa gcgattgtgg
      781 cgcttacccg acagttacaa attaccctac gcggtagcga tgtgattggt cggtttggcg
      841 gcgatgagtt tgcagtaatc atgtccggta cgccagcaga gagcgccatc accgccatgt
      901 tacgggtgca tgaagggcta aacacgttac gcttgccgaa tacgccacag gtaactttac
      961 ggattagtgt gggggttgcg ccgctgaatc cacaaatgag tcactatcgc gagtggttga
     1021 aatcggcaga tttggcgctc tacaaggcaa agaaagccgg acgtaaccgc accgaagtgg
     1081 cggcctgacg tccggcaaaa gccatcagga tttgctgagt ttttctgatt tttccataca
     1141 cttcgtcatc gcttcgatca ctgcggcacg gaagcctttc tcttccagta cgcgtaccgc
     1201 ctcaatggtg gtgcctcccg gtgagcagac catatctttc agtgttcccg gatgttctcc
     1261 cgtttccagc accatttttg cggagcccat taccgcctga gcggcaaatt tatacgcctg
     1321 ggcgcgtggc atcccgccca gcacggcggc gtcggccatc gcttcgataa acataaagac
     1381 gtaggctggc gaagaaccgc tcacaccgac caccgagtgg atcatcggct cagcaatcac
     1441 ttccgcttcg ccaaagcagc ggaaaatatt cagcacatca gcggtatctt ctggggttac
     1501 cagcgcgttt ggcgttacgg aggtcatacc ggcattaacc agcgcgggag tgttcggcat
     1561 ggcgcggata attttccggt catggcccag cgcgcgggca agctggtcga gcgtgatacc
     1621 tgcagcaata gaaacgacca gagagtcttt attcaggctg gaggtgattt cgctaagtac
     1681 tttaatcatg atgcctggtt taacggcggc aaaaatgatg tcggcgattt gcgctacttc
     1741 ttgcgccgat tctgcggcgt tgatgccgaa ctggtcatgc agggcggtga ctttatccgg
     1801 ggagggggta tatacccaga tttgccctgg aagcacctga ccgctggcaa tcagaccgcc
     1861 gagaatggct tttcccatat tgccgcagcc aataaaaccg attttctttt ccattgcctc
     1921 actcctgccg tgaaattcat tgttttgata atcgctggca gaagcataaa cagaactatg
     1981 ccggaaggca aaagcgcgac acaatagagg attacccaac aaaggatgac tttatgacaa
     2041 tttgggtgga tgccgacgcg tgtcccaatg taattaaaga gattttgtat cgcgcggcgg
     2101 aacgtatgca gatgccgctg gtactggtag caaaccagag tttacgcgtg ccgccatcgc
     2161 gatttattcg tacgctgcgc gtcgcggcag gtttcgacgt tgctgataac gaaattgttc
     2221 ggcagtgtga agcgggcgat ttggtgatca ccgcagatat acctttggct gctgaagcca
     2281 tcgagaaagg cgcggcggcg cttaatccgc gcggcgaacg ttacacgcca gcgaccattc
     2341 gtgagcgcct gacgatgcgc gattttatgg ataccttacg tgccagtggg atccagaccg
     2401 gaggaccaga tagtctttca caacgtgacc gccaggcttt tgctgcggag ctggagaagt
     2461 ggtggctgga agtgcaacgt agtcgtggct aaatgtaatt tattatttac acttcattct
     2521 tgaatattta ttggtatagt aagggatgta ttgagatttc caccttaagt ggaatttttt
     2581 ctttacaatc gaaattgtac tagtttgatg gtatgatcgc tattctcatg acaccggctt
     2641 tcgccgcatt gcgacctatt ggggaaaacc cacgatgaca caacctcttt ttctgatcgg
     2701 gcctcggggc tgtggtaaaa caacggtcgg aatggccctt gccgattcgc ttaaccgtcg
     2761 gtttgtcgat accgacctgt ggttgcaatc acagctcaat atgacggtcg cggagatcgt
     2821 cgaaagggaa gagtgggcgg gattccgcgc cagagaaacg gcggcgctgg aagcggtaac
     2881 tgcgccatcc accgttatcg ctacaggcgg cggcattatt ctgacggaat ttaatcgtca
     2941 cttcatgcaa aataacggga tcgtggttta tttgtgtgcg ccagtatcag tcctggttaa
     3001 ccgactgcaa gctgcaccgg aagaagattt acggccaacc ttaacgggaa aaccactgag
     3061 cgaagaagtt caggaagtgc tggaagaacg cgatgcgcta tatcgcgaag ttgcgcatat
     3121 tatcatcgac gcaacaaacg aacccagcca ggtgatttct gaaattcgca gcgctctggc
     3181 acagacgatc aattgttgat tttcgagcgc ctatacttaa cgttcatccc gtgaaataag
     3241 gaagaacgat gccaacgaaa ccgccttatc ctcgtgaagc atatatagtg acgattgata
     3301 aaggaaagcc aggacagacg gtaacctggt accaactcag agccgatcat cctaaaccag
     3361 actcgttgat cagtgaacat ccgaccgctc aggaagcgat ggatgcgaaa aaacgctatg
     3421 aggaccctga caaagagtga ccgcatcaga ctgctcggaa gggattctga gtgccgccac
     3481 aagggatctg cgtcacattt ttcacaattc atgtttttca aataattaga atattaaaca
     3541 ataacaat
//
LOCUS       DAZFRG010000353         3541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFRG010000353 DAZFRG010000000
VERSION     DAZFRG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.355"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHKUC_004746"
     CDS             <1..216
                     /locus_tag="ACHKUC_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO6620836.1"
                     /translation="SERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLE
                     DYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            372..1907
                     /gene="gadC"
                     /locus_tag="ACHKUC_004747"
     CDS             372..1907
                     /gene="gadC"
                     /locus_tag="ACHKUC_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO6620837.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2038..3357
                     /locus_tag="ACHKUC_004748"
     CDS             2038..3357
                     /locus_tag="ACHKUC_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO6620838.1"
                     /translation="MDICSRNEKLAIRRPAILGALALLLCSCKSTPPESMVTPPAGSK
                     PPAMTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          866 a          886 c          861 g          928 t
ORIGIN      
        1 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
       61 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      121 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgaccaccc gaaactgcag
      181 ggtattgccc agcagaacag ctttaaacac acctgataac gtttaacggt aacggtgtcc
      241 cgaaacgaag ccgtttcggg acaatttcca aagtctgttc actggcatta gcaacggaaa
      301 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      361 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      421 gattttttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      481 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      541 tgggactttg tgctgcggaa atggctaccg tcgacggctg ggaagaaggt ggcgtcttcg
      601 cctgggtatc aaatactctg gggccgagat ggggatttgc agcgatctca tttggctatc
      661 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      721 tcctgaaatg gccagctctg aatgaagacc ccattaccaa aactattgca gcactcatca
      781 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      841 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      901 tttatctgca ctccggtgcc cccgttgcta tcgaaatgga tgcgaagacc ttcttccctg
      961 acttctctaa agtgggcacc ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1021 ttgaagcttc cgcaacccac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1081 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1141 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1201 ttctgatgtc acatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1261 tgttgctggg tgttctggcg gaaatcgctt cctggattgt tggtccttct cgcgggatgt
     1321 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1381 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1441 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1501 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1561 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcta
     1621 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1681 tccagggtga ctctaccgat atgtatgttg aattactggt cgttagtttc ctggtggtac
     1741 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aagcaaagcg aataccggcg
     1801 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1861 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1921 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgtt gtcgtattga
     1981 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2041 gatatctgct cccgaaacga gaaattagcg attagaagac cagcgatact gggtgcactg
     2101 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagcg
     2161 ggttcaaagc caccagccat gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2221 gccacggtgt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taaccccacc
     2281 agccgggcac gtgtacaaca acaggcgatg atcgacaaac tggatcatct gcaacgtctc
     2341 ggcataaaca cggtcttttt ccaggtcaag ccggatggta ccgccctgtg gccatcgaaa
     2401 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2461 ctgcaattta tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2521 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcaccctg
     2581 tctcaacaac cggccagcgt ctatgtgcaa caccgcgact ggatcagaac gtccggcgat
     2641 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgcg
     2701 gaagtggttt cccgctatcc agtagatggc gtgcagtttg atgactattt ctatactgaa
     2761 tcaccaggtt cacggctaaa tgataacgaa acgtaccgca aatacggcgg cgcatttgcg
     2821 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcgaaggt atcgcacacc
     2881 attaaaagca ttaagccgga agtcgaattt ggcgttagcc cagcaggcgt gtggcgtaac
     2941 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3001 gctgataccc gtcgatgggt ggaacaaggg ttgctggatt acattgctcc ccaaatttac
     3061 tggccattct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3121 gttaaaccga ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3181 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3241 gatcttaacg atgctgtgcc ggaaattagc ggcacaatct tgttccgtga ggactatctg
     3301 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3361 cccattctta ctttgtaaca agccggtgct ttacccactg gcttgttacc ctcttgaact
     3421 atcccactag caatcatgct taaatacgtc aaattaacca cgcaattaac cacacttagt
     3481 taaacagcca taattcccct aataaaaatg tatgaatatg aaaaaacata cgatattcgc
     3541 t
//
LOCUS       DAZFRG010000354         3540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFRG010000354 DAZFRG010000000
VERSION     DAZFRG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.356"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..732
                     /locus_tag="ACHKUC_004749"
     CDS             <1..732
                     /locus_tag="ACHKUC_004749"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6620839.1"
                     /translation="KPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAK
                     GSALKQHVMAPLISYFRDARAALGITAKQIADVTGKKNMVSHWFSASQWQLPNESDYL
                     KLQSLFARVAEEKHQRGELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQFYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALG
                     RRAIGVELETGRFEQTVREVQDLIV"
     gene            782..857
                     /locus_tag="ACHKUC_004750"
     tRNA            782..857
                     /locus_tag="ACHKUC_004750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:815..817,aa:Ile,seq:cat)
     gene            865..941
                     /locus_tag="ACHKUC_004751"
     tRNA            865..941
                     /locus_tag="ACHKUC_004751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Arg,seq:tcg)
     gene            955..1031
                     /locus_tag="ACHKUC_004752"
     tRNA            955..1031
                     /locus_tag="ACHKUC_004752"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:989..991,aa:Arg,seq:tct)
     gene            complement(1135..1284)
                     /locus_tag="ACHKUC_004753"
                     /pseudo
     CDS             complement(1135..1284)
                     /locus_tag="ACHKUC_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1473..1688
                     /locus_tag="ACHKUC_004754"
     CDS             1473..1688
                     /locus_tag="ACHKUC_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6620840.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKAARGE"
     gene            1693..2043
                     /locus_tag="ACHKUC_004755"
     CDS             1693..2043
                     /locus_tag="ACHKUC_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6620841.1"
                     /translation="MIQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALGFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            2107..2640
                     /gene="rrrQ"
                     /locus_tag="ACHKUC_004756"
     CDS             2107..2640
                     /gene="rrrQ"
                     /locus_tag="ACHKUC_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6620842.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIEQ"
     gene            2857..3042
                     /locus_tag="ACHKUC_004757"
     CDS             2857..3042
                     /locus_tag="ACHKUC_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6620843.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISLSESG"
BASE COUNT          865 a          840 c         1010 g          825 t
ORIGIN      
        1 aagccgtccg gacgctggaa cggatgcaac aaggaaagcc tgcgggcgta tttccccgcc
       61 acagagcgca ttctgttcgc ggaacattat caggggccgt atcgtccgaa agatgccggg
      121 tatgcggcga agggcagtgc actgaaacag catgtgatgg ccccgctgat ttcttacttt
      181 cgtgatgcgc gcgcggccct ggggataacg gcaaaacaga ttgcagatgt cacaggaaag
      241 aaaaacatgg tgtcgcactg gttcagtgcc agtcagtggc agctaccgaa cgaaagcgat
      301 tatctgaaat tacagtcgct gtttgcccgg gtggcagaag agaaacatca gcgcggtgaa
      361 ctggaaaagc cccaccacca gctggtggat acgtatacgt cactgaaccg gcagtatgtg
      421 gagctgcaga gtgaatataa gcatctgcgg cggtattttg gtgtgacggc gcaggtgccg
      481 tacacggatg tatggacaca taaaccggtg cagttctatc ccgggaaaca tccgtgcgaa
      541 aaaccggcag aaatgctgca gcagataatc agcgcaagca gtcgtccggg tgacctggtt
      601 gcagattttt ttatgggctc aggttcaacg gtaaaagcgg cactggcgct cgggcgtcgt
      661 gcgattggcg ttgaactgga gaccggacgt tttgagcaga cagtcaggga agttcaggat
      721 ttaatcgttt gaaacggatg agattgcaga attaattacg caccattatt attctgctcc
      781 cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa
      841 tccagcaagg gccaccatca cataccgcca ttagctcatc aggaaagagc gccagccttc
      901 gaagctggtt gcgcggagtt cgggtccccg aaggcggtcc attatctgta tcctgcgttg
      961 ttagctcagc cggacagagc aattgccttc taagcaatcg gtcactggtt cgaatccagt
     1021 acaacgcgcc acacttattt tccctggctc gcttttgcgg gctttttttt aaatgtctca
     1081 caattcaggc ggttgactgt tgtctggttt gcggggagtt tgttaaaaga aactggcatg
     1141 gtgaatcccc ctgtgcggag gggcaatcag cgagtaggta tatgggataa tcgcggattc
     1201 aggtgctggt actgaattca ccgggaggca cccggcacca tgcagttgac tgaaaccatg
     1261 tatactctca ggccccacgc acatgtgttg gggccttttt acatgcaaaa aaaaagcccg
     1321 catatggata cgggcggcaa ggaacaaaaa acgtgaagtg atctattcag ccagtggata
     1381 ataccctgac gttaccatat tgcgcaatcg cgcattcttt ctttttcgct ccccttataa
     1441 ctacgccatc cgttcgctgc ggaggtgagg ctatgaaatc catggataaa atttcaacgg
     1501 gcattgccta cggcacctcc gcaggcagtg ctggctactg gtttttacag tggcttgatc
     1561 aggtcagtcc gtcacagtgg gctgcgattg gtgtactggg gagtctggtt ctgggcttcc
     1621 tgacatatct gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gctgcgcggg
     1681 gagagtaatt caatgattca aaactatgaa ctgattgtga aagggatccg caattttgag
     1741 aataaagtta cggtaacttt agcgttacgg gacaaaaaac gctttgacgg tgaaattttt
     1801 gacctggaca tctcgctgga ccgtgttgaa ggtgccgcgc tggggtttta tgaggcagca
     1861 gccagaagga gcatcagaca ggtcttcctg gatgttgctg ccgggttatg tgaaggggat
     1921 gagcagtcgc cggaaaagcg ccccgtaatt ttagaggcgc agaatgtgtg gataacctac
     1981 aaaggaaagc taccaggaag aattactggt tctctgaaga ctcctccgga atcacaacct
     2041 taagtcactg accagaacag ataaacctgt ccgtgggcag aaaccgataa atcctgataa
     2101 atatccatga acgcaaaaat cagatacggc ctgtcggctg ccgttctggc actgattgcc
     2161 gtcggtgcgc ccgcgcctga tattctcgac cagtttctgg atgaaaaaga aggtaaccac
     2221 acaacggcat accgcgatgg ttccggcatc tggaccatct gccgtggtgc catcatggtg
     2281 gatggcaaac ctgtcgttcc gggcatgaag ttgtcgaagg aaaaatgcga ccaggttaac
     2341 gccattgagc gtgataaagc gctggcgtgg gtggagaaaa acatcagagt gccgctgacc
     2401 gaaccccaga aagcggggat cgcgtcattc tgtccgtaca acattggccc cggtaagtgt
     2461 ttcccgtcga cgttttataa gcggctgaat gccggtgatc gtaagggggc atgcgaagcg
     2521 attcgctggt ggattaagga tggtgggcgc gattgccgca tacgttcaaa taactgctat
     2581 gggcaagtta ttcgtcgtga ccaggaaagc gcattagcct gttgggggat agagcagtga
     2641 gcagagtcgc cgcgattatt tatgctctgg ttatctgcac catcgtctgc ctgtcgtggg
     2701 cggtcaatca ttaccgtgat aacgccattg cctacaaaga acagcgtgat aaaaaagtca
     2761 gtgagctgaa gcaggcgaac gccaccatcg ctgacatgca gcagcgtcag cgtgatgttg
     2821 ctgcgctcga tgcaaaatac tcgagggaat tagccgatgc gagagctgaa aatgaaactc
     2881 tgcgtgctga tgttgccgct ggtcgtaagc gcctgcggat caacgccacc tgctccggta
     2941 ccgtgcgtga agccaccggc acctccggcg tggataatgc aaccggcccc cgactggcag
     3001 acaccgctga acgggattat ttcactctca gagagcggtt gatgctgatg cagaagcagc
     3061 tggaagtggc gcaggaatat atccgcactc agtgcctgaa ataagttttg ctgatgcgcg
     3121 gtattgtcgc cgtattcctg cattaacaga gaccgcagcc cgacagggag actcctctgc
     3181 gcgagtgtgc gggaataatc aaaaacgatg cacaccgggt ttttaccgcg tttatggttc
     3241 gcgggtttgt ctctcatgct cgccagtcct gtgcgggggt ggaagaaaca ggacacttac
     3301 acagattctt gtgggcacga tgctatgcct ttctggatta tcccgatgcc attcatgcaa
     3361 ggcgttgtat cagacgttcg tcagagctgt caggctgacg ggtcctcccg gtggggtggc
     3421 ctgccacggg gcgggagcgt cgcggaaaaa gactagtttt tgcatttcca tggcggcggc
     3481 agcatgtttg gtaatttatt gataattaaa agttatttct cttttcacct gtacaatatt
//
LOCUS       DAZFRG010000355         3540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFRG010000355 DAZFRG010000000
VERSION     DAZFRG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.357"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..871
                     /gene="hprS"
                     /locus_tag="ACHKUC_004758"
     CDS             <1..871
                     /gene="hprS"
                     /locus_tag="ACHKUC_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6620844.1"
                     /translation="ICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVE
                     VNALPRELKPLGQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQT
                     RSIAEYQKTIAGNIEELENISRLTENILFLARADKNNVLVKLDALSLNKEVENLMDYL
                     EYLSDEKEIRFKVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGS
                     LNIDIASPGTKINEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLS
                     KHNVFRITLPQRN"
     gene            complement(979..1830)
                     /gene="hchA"
                     /locus_tag="ACHKUC_004759"
     CDS             complement(979..1830)
                     /gene="hchA"
                     /locus_tag="ACHKUC_004759"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO6620845.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2426..3484)
                     /locus_tag="ACHKUC_004760"
     CDS             complement(2426..3484)
                     /locus_tag="ACHKUC_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO6620846.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERIL
                     TDRESGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQS
                     GDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAVGIVYQF"
BASE COUNT          983 a          735 c          812 g         1010 t
ORIGIN      
        1 aatttgcatt gtcgccatta tattgtgctc agtattaagt ccgttgttaa tcagaacggg
       61 gttgcgagag atcaaaaagt tgagtggcgt aacggaagcg ctgaattata acgatagccg
      121 agtgccggtt gaggttaacg cattaccgag agaactaaaa cctcttgggc aggcgttgaa
      181 taaaatgcat catgctttag tcaaagattt tgagcgcctt agtcaatttg ctgacgatct
      241 cgctcatgaa cttagaacgc caattaatgc attactgggt cagaatcagg ttacgctcag
      301 tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggaaacattg aagagctgga
      361 aaatatttcg cggttaacag agaatatact gtttcttgcc agggcagaca aaaacaatgt
      421 tttggtgaaa ctggatgcgc tttctctcaa taaggaagtt gaaaatctta tggactatct
      481 tgaatatctt tcagacgaga aagagattcg ttttaaggtc gagtgcaatc aacaaatctt
      541 tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaacg ccattcgata
      601 ttcaccagaa aaatcgcgta ttcatataac cagttttctt gatgccaacg gctctcttaa
      661 tattgatatc gccagtcctg gaacgaaaat taatgagcct gaaaaactct tccgtagatt
      721 ttggcgggga gataattcgc gtcattccgt aggtcaggga cttggccttt ctttagtcaa
      781 agcgattgcc gaattacatg gaggaagtgc tacgtatcac tatctcagta agcataacgt
      841 gttccgaatt acgttaccgc aaagaaatta acatcgggct tcagtacgcc ataagttaac
      901 cgtgaaaaaa cgcagccacg tcgtgagtat taacgtggct gcgattgatt atgcgcttac
      961 attacaatgt aacagggatt aactcgcgta agctgccagc atttcctgcg ccgccagttt
     1021 acccaacgca ttcgctgcaa aaggactgtc gccggtgaga agtttacggt ccttatgtac
     1081 tcgcccggtg atgtcgtcat taatgatgtt catgcccatt ttcttcaact cttcgccgaa
     1141 gtaccaggtg agatgacccg gcatatagcc aatatctggc gtttgtttgt ctgcggcgtc
     1201 tgggaatgcg caaatagaat aaccattcag tgggttatcg ctgtggcgaa gtgccagaaa
     1261 agccgccgga ccgtggcaaa gggagataac aaaacggtca tttttgattg cccactgtaa
     1321 agcggcagcc acgtcctggc tttctggtaa accaataagt gcgccatgcc caccaggaac
     1381 aaagattgct gcatattcgc tatcagcgtt gaggctggcg acaacatccg cgagtttctt
     1441 cggattacgg aataacgatt tatgctgttc aaagaatggc attacttttt catctttgtg
     1501 cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca cttcgaattc
     1561 gaaacctgca gcatggagat gatacaacgg cagcaacgtt tcaatcggat ggttaccggt
     1621 cgagaacagt tttccgttat cggtcggcaa ataacgttcg tccgcggcga tcaccagaat
     1681 tttatgttta ccgcgatacg gttttggata gtccacgcca tcaagatcag agacgggact
     1741 cgtatattgg ctaagcgaat attctgaagg gaagaacgca ttatcttcag caatatcgac
     1801 ctgcgggttt ttacttgttt gaacagtcat agtgtattcc ttattgttgc ttagttaggg
     1861 tagtcactat atttgcgact gagctggtcg ctatttttaa ccaggctgaa aaattgagtc
     1921 aatatatcga aagcggtagt tggcgggcat aagtatgtaa tgatgatgct gcactggtgt
     1981 tgaatattat tcacgccaaa ctttccttat tgagtaacgt cgtcgcaagt ggcctcaatg
     2041 attttagacc attatcagag gtttgggggc gatggggaga gatttagtgc tgtggaatgt
     2101 tgtggtactg atgaatattt atatcccgga ttggtaggga aaaagctttt ttcatgcact
     2161 atttttgcaa atatttctat atgggaagcg gttttgttac agattaaatt tataaagaaa
     2221 tgcatttggg gtgttggctg gaagagttgc tggtagagga tattgtgcca ggaggggcag
     2281 ggtaaggggc tgtatcgaga tgctttggca attgcgtgtg ccgcagatgg agatgaaatg
     2341 ccaataaaaa aagcctgagt tcgatgaggg acgaactcag gctggtgtta tgttttacag
     2401 taacacgcct cggcgcgttg gctgattaga actggtagac gataccaact gccagttggt
     2461 cgtcggtgtt cagtccatga gcgagaacat aatcactctt atcaagcagg ttgatctgat
     2521 aagcggtata tacgttcata ttcttattga agtaatacca ggtaccgatt tcaatatagt
     2581 tcagacgatc agaatctttg taacctgcta cgtccagtgc tttagaatag gagtaaccga
     2641 tggatggacg cagaccaaag tcgaactgat attgcagaac agcttcgaag ttttgtgttt
     2701 tgttcacaac atcaccgtcg tcggaattca tgttgtgaga ttcaccgtac atcacagcag
     2761 catagacatt gttagcgtca tattttgttg acaacgccca aacttcggct ctttcgcctt
     2821 taccatcacc agactgccaa tcagtacggt cagagtttgc ataagcacca gcaaatgaga
     2881 aaccgtcaat agtgtaagtt gcagataaac catggccatc gccatttgct ttgtagttgc
     2941 ctgtaccttc atttttaccc tgatattgca gagcaaaatt tagaccttca accatgccaa
     3001 agaaatctgt gttacgatag gttgcaacac cgttggtacg gttggtcatg aacaggtcag
     3061 taccagccca tgagtcaccg ccccactcaa caaacatatc agtaaatgct tctgcatcat
     3121 acgcgacacc gacattacga ccgtagtcga atgaaccaaa atctttgtaa ctcaaaccag
     3181 catatgccaa acgagtttgg tcagggttgt gtctgtttga tgcttccagg tctagttcaa
     3241 actgaccgta accagtcagt tcagggttga tctgagtttc acctttcacg cctacacgag
     3301 cataagaggt atcttgagaa ttatcgcctt tttcaccact ttcacggtcg gtcagaatgc
     3361 gctcaccaac catcttgccg tatagctcaa ctttattgcc atttttatta taaatttcag
     3421 ccgcatttgc tgcgccagca actaataacg ccgggaccag cattgccaga acttttcttt
     3481 tcattatgta ttcccttgtg attataatct tcatgaatat atcaataagt gctgttatcc
//
LOCUS       DAZFRG010000356         3515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFRG010000356 DAZFRG010000000
VERSION     DAZFRG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.358"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(194..1372)
                     /locus_tag="ACHKUC_004761"
     CDS             complement(194..1372)
                     /locus_tag="ACHKUC_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6620847.1"
                     /translation="MPLTVRQIESAKPADKDYKLSDGQGLYLLVKKSGSRYWNLKYRF
                     AGKEKKLVIGVYPDISLAQARQKRDEARKLLAEGKDPSYEKQALKFAIRESMACTFRT
                     IAEELLTHMARRWTAGYQADQRARLENHVFPALGNRPVTGIRPMEILNVLRVIEEQGK
                     LEALRKTRQLCAQVFDYAIATGRATVNPASTLSGVLLPPEKQQRRTLSEEQMGAFMHQ
                     MTASGNLMSLATRMLVLTAVRTGELRLASWDEINFTEALWVIPKERMKMRRPHVVPLS
                     RQVLALLKEIKTRTESRHSAFIIPGAFRKDRPRGRKCINEFLEQSGWHALTTAHGFRH
                     TFSTILHDHDFSSAWIELQLAHVDKNVMRGTYNHAQYLDGRREMMQWYADYIDSLSSQ
                     "
     gene            complement(1601..2347)
                     /gene="glnH"
                     /locus_tag="ACHKUC_004762"
     CDS             complement(1601..2347)
                     /gene="glnH"
                     /locus_tag="ACHKUC_004762"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO6620848.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(2751..3254)
                     /gene="dps"
                     /locus_tag="ACHKUC_004763"
                     /gene_synonym="pexB"
     CDS             complement(2751..3254)
                     /gene="dps"
                     /locus_tag="ACHKUC_004763"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO6620849.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT          910 a          812 c          820 g          973 t
ORIGIN      
        1 gccggaataa gtgcaaggcc atattctgta atcaccagcc tgaaaatgtc aatttgtctg
       61 caatcatgtt ttttgtgata attttgcaga tgaaaaaata taattaataa caaaacccct
      121 ccggccctgc aggccggagg gtaaaatact taaccaggaa aaagtatcat ggaaaagtat
      181 ttctgactta actttactgg ctggagagtg aatcaatgta gtcggcgtac cactgcatca
      241 tttcacggcg accatcgaga tactgcgcgt ggttataggt gccacgcatg acgtttttat
      301 caacgtgggc aagctggagt tcaatccagg ccgagctgaa atcatggtca tgaagaatgg
      361 tactgaaggt atgccgaaaa ccgtgagctg ttgtcagggc atgccagcct gactgctcaa
      421 ggaattcatt aatacactta cggccccggg ggcggtcttt cctgaatgcc ccgggaataa
      481 taaaggcgga atgacgcgat tcagtgcgtg tttttatctc cttcagtaac gccagtacct
      541 gacgtgaaag cggcacaaca tgcggtcgtc gcattttcat tctttctttt ggaataaccc
      601 acagggcctc cgtgaagttt atttcatccc agctcgccag ccgcagttct ccggtgcgaa
      661 ccgctgtaag aaccagcatt cgcgttgcca gtgacatcag atttccggaa gccgtcatct
      721 gatgcatgaa agcccccatc tgctcttcag aaagtgtcct gcgctgttgt ttttcaggag
      781 gaagcagtac cccgctgagt gttgacgctg ggttaacagt tgcacgtcct gttgctatgg
      841 cataatcaaa aacctgcgca cagagctggc gtgttttacg cagtgcttca agttttccct
      901 gttcttcaat caccctcagg acgttcagta tttccatggg acgtatcccg gtcacaggac
      961 ggtttccaag ggcagggaaa acatgatttt caagtcttgc acgctggtct gcctggtaac
     1021 ctgctgtcca tcgacgtgcc atatgggtaa gcagttcctc tgcaatggtg cggaatgtgc
     1081 aggccatact ctccctgatg gcaaatttta gcgcctgttt ctcatatgac gggtcctttc
     1141 cttcagccag gagtttacgc gcttcatccc gtttctgacg cgcctgagcc agagatatgt
     1201 caggatagac accaatcacc agtttctttt ccttaccggc aaaacggtac ttcagattcc
     1261 agtaacggga accggatttt ttgacaagaa gataaagtcc ctgaccgtcg gaaagtttgt
     1321 aatccttgtc agcgggttta gctgactcaa tttgtcgaac agtaagtggc ataatgttaa
     1381 tcccgaaaag tagcccccat aatagccccc aaaattatct tgatgcacat ggaagatggt
     1441 tgacatccag agaggagaga tttctgataa tgccttattt taaaagctgt tgaagggaga
     1501 ggatagagtt ctgttgaaat cagttgatag caacagaact taatagatcc tccccggcgg
     1561 ggagagggaa ctgccagtgc gctttacagg cgccgcgtta ttatttcggt tcagtaccga
     1621 accatttttt gtagatttcg ttgtaagttc cgttctcgcg cagggttttc aacgcgccgt
     1681 tgactttgtc acgcagctcg tcgctacctt tcgggaacgc aataccgtat tgctgtgctt
     1741 ccagagagtc acctaccgct ttgaactgac cgttaccggc ggttttgatg aagtacagaa
     1801 tgtttggcgt atcgtgcaga acggcgtctg cgcggttggt gcccagttcc atataggcgt
     1861 tatcgatatt cgggaactga cgcagatctt tagttttgat gtttgctttc gcgtaatcaa
     1921 cggagccagt gccgctcttc acagcaacca ctttcccgtc gagatctttc acgcttttca
     1981 catcgttatt gttagctttc accatcacta acaggccgct tttgtagtag ccgtcagaga
     2041 aatcgatcgc ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc agatcgacgt
     2101 ttttggtttg cagtgctgga atgatcccac tgaaatccat cggcttcagt tcataatcca
     2161 gcttcagttc tttagcgatg gcagcccaca gatcaacgtc aaagcccacg tatttatcgc
     2221 cctgtttaaa ttcaaacgga acgaaggcag tatccgtcgc gacaactaat tttttatccg
     2281 cggcatgaga agaaaccgca aaagccaggg tcagtgcagc cagtgaaact tttaatacag
     2341 acttcatagc atttcctttt ttctatccac gggacgatcc cctgcgtgac attcacatat
     2401 atgaaaaaat cgtgccagtt ttgcaactca ttgatttttc gagatgcggc aaaaacgtga
     2461 tgcacgattt atagggcaat accctgaaga tgcaccattc tggggcacca aactggtgcg
     2521 ctaaaattgt gcactcaaca cagtgattat ttagcgtaaa acctgatgaa aaaacaatct
     2581 ttatgtaacg attgtgtgat gatgtggata caaaaaattt acttaaccag ctggtgatag
     2641 cagatggatg cactaaataa gtgcgttgag gcgggctaca aaacaaaacg gcctcctgtc
     2701 aggaagccgc ttttatcgtg tactaaagtt ctgcaccatc agcgatggat ttattcgatg
     2761 ttagactcga taaaccacag gaatttatcc aggtcgcgag acgcggcggt caggatatct
     2821 gcggtgtcgt catctttcgc ttcgccaatc gctttgcgta cgtcattagc gacgattgcg
     2881 taacggtcag ccagctcttt caggtgatcc tgaacgttgt ggatgtccag cgggtaactt
     2941 ttcagcggag ttttgctgtt gataacttga gtggtcccca gagctacacc gcccagctgc
     3001 actgcacgtt ctgccatggt atccagatga tcgatcagtg cagtgcggaa gccatccagc
     3061 atttcatgta cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg tttggtaatc
     3121 aaagaaagat caataaactg gataacctgg cgattcagca actctactgt tgcttttttc
     3181 tcgctgtcag agacatcgtt gcgggtataa agcagattgg tcgcttttga tttaactaat
     3241 ttagcggtac tcataatttc atatcctctt gatgttatgt cccagtaatt aacgagacta
     3301 agtatagcac cagctatgtg ttccgctatt ctggctgttc ctatcacact aatagtggta
     3361 acaagcgtga aaaaacaaaa ccaataaaaa ctcaatgagt tagatatatt aataagaaca
     3421 attcttattt atttgctcgc caggaatttt cggaataaat atgcttttgc ataattcgat
     3481 atataaagac ggcgtagagg aaaagtagcg agaaa
//
LOCUS       DAZFRG010000357         3499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFRG010000357 DAZFRG010000000
VERSION     DAZFRG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.359"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..379)
                     /locus_tag="ACHKUC_004764"
     CDS             complement(92..379)
                     /locus_tag="ACHKUC_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO6620850.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(454..597)
                     /gene="mgrB"
                     /locus_tag="ACHKUC_004765"
     CDS             complement(454..597)
                     /gene="mgrB"
                     /locus_tag="ACHKUC_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO6620851.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            756..995
                     /locus_tag="ACHKUC_004766"
     CDS             756..995
                     /locus_tag="ACHKUC_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO6620852.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHKUC_004767"
     CDS             complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHKUC_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO6620853.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2107..3480
                     /locus_tag="ACHKUC_004768"
     CDS             2107..3480
                     /locus_tag="ACHKUC_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620854.1"
                     /translation="MPIVQADGLPLPQRYCAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          839 a          883 c          783 g          994 t
ORIGIN      
        1 taatttgaag cagctgcacc atgacagtgc gtataatcag catgaattga gtaacccttc
       61 aacgacgggg ttactcaaga ttgaggaagg attatcgctc ggcgaccaga cgaataatat
      121 cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc tcaggttcgt
      181 ttgcttcgca caaacgacgt aacagtgcat tttgacgttt ttgctgatcc agcaacgcct
      241 caagcagttc aatctgttcg ttagtccgtg aactggcacg attgataaaa aaccacaaga
      301 tgagcccgat aagaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg
      361 aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta ccactgctgg
      421 agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc
      481 acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca
      541 aagcagcaag caagccaaca ccacgacaac cagagcgacc catcgaaact ttttcactcc
      601 aatctccgtt tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta
      661 actatgtcga tattcgtgct ttttcggaat gagtcgcttg cttcagcgca tattgccgct
      721 acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt cgcacagtta
      781 tgctgatagc gctggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa
      841 gcaaagagaa tgccgcggga ttaggtttac aatgtaccta tctgaccgcc agaggcacca
      901 gcacggtaca atacctgcac actaaaagtg gttttctggg gataacagat tgcccgttgt
      961 tacgtaaaag caatattgtg gtcgataatg gttaagggta ataaataacc agattctcac
     1021 tcatgagtaa aatattctta tcgcaagtgc gtttaatata acgccacctt caactttatt
     1081 tttctcatgc taaataaaga acctgtctgt ttgatattca gacaggttct tactttcctc
     1141 agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgccgcg gtatgcaaca
     1201 ttgcgacata ttcctgtaaa cgctcttcag agaaacgcaa cgtcgggaag gagatgctca
     1261 gaccggcaat gaccacgcca aagcgatcaa ataccggaac cgcaatgcaa cgcagtcctt
     1321 cttcctgctc ttcattatct tcgccatacc cctgctcgcg cacctggtcc agaacgggta
     1381 ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc acgccctcaa
     1441 gaatttgcat cacttcatcg cgatcgcgcc atgccagcag taccttacca atcgcggtgc
     1501 tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac attgagtcaa
     1561 ttttgtggat gtaaacaata ctatcttcgt ccagtgcgcc gaggtggata gtttctttgg
     1621 tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg acgttttgca
     1681 acgcgcgagc gcccagttca aacaatttca gggtcagcga atatttctcc gactcccctt
     1741 cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg gtgctttttg
     1801 acatcatgac gcgctgcgac aactcggtta tccctatttc gcgctcttca cccagcgcct
     1861 gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta tccagatctg
     1921 cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg ttttttatta
     1981 taatttcgca ccagggtggt cgcaatccat cttttgccgg ttagttacaa ttctgcgaca
     2041 tccaccgtga atatcagtgc tagaatcata cccctgttga ttattcacca aagatataaa
     2101 attcctatgc caatagttca ggccgacggc ctgccattgc cccagcgata ctgtgcgata
     2161 ttaaccattg tgattggtat ttcgatggct gtccttgacg gcgcaatcgc caacgtcgcc
     2221 ttgccaacaa tcgctacgga ccttcatgcc acgccggcca gttccatctg ggtagtgaac
     2281 gcctatcaaa tcgccattgt catctccctg ctctcgtttt cgtttctggg cgatatgttt
     2341 ggctatcgac gtatttataa atgcggtctg gtcgtttttc tgttgtcttc actgttctgc
     2401 gccctttctg attcgctgca aatgctcacc cttgcgcgtg tcatacaagg tttcggcggt
     2461 gcagcgttga tgagcgttaa taccgcactt atccgcctga tctatccaca acgttttctg
     2521 ggtagaggga tgggcataaa ctcgtttata gttgccgtct cttctgctgc cgggccgaca
     2581 attgctgcag caatcctctc catcgcatcc tggaaatggt tatttttaat caacgtaccg
     2641 ttgggtatta tcgccctgct tctggcgatg cgttttctgc cacccaatgg ttctcgcgcc
     2701 agtaaacccc gtttcgacct gcccagcgcc gtgatgaacg cgttaacctt cggcctgctt
     2761 attactgcat tgagtggttt cgctcagggg caatcactga cattgattgg tgcggaactg
     2821 gtggtaatgg ttgtcgttgg tattttcttt attcgccgcc agctttctct tcccgtaccg
     2881 ctgctaccgg tggatttact gcgtatcccg ctgttttcac tttctatttg cacatctgtt
     2941 tgctctttct gcgcacaaat gctggcaatg gtttcccttc ccttttacct gcaaaccgtg
     3001 ctcgggcgta gtgaagtcga aacaggttta cttctgacac cgtggccgtt agcaacgatg
     3061 gtgatggctc cactggcagg ctatttgatt gaacgcgtac atgcaggatt gctgggtgct
     3121 ttagggttat tcatcatggc tgcggggctt ttttccctgg ttctgctgcc cgcgtcacct
     3181 gcggatatca atattatctg gccgatgatc ttatgtggcg ctggatttgg cttgttccag
     3241 tcacccaata accacaccat tattacctcc gctcctcgcg aacgtagcgg tggagccagt
     3301 ggcatgttag ggacggctcg tcttctgggt cagagtagcg gcgcggctct ggtagcgctg
     3361 atgctaaatc agtttggtga taatggtacg cacgtctcgc tgatagctgc ggctattctg
     3421 gcagtgattg ctgcctgtgt cagtggttta cgtatcactc agccacgatc cagggcataa
     3481 aaaaagcgcg tcgatcagg
//
LOCUS       DAZFRG010000358         3416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFRG010000358 DAZFRG010000000
VERSION     DAZFRG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.360"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            140..1105
                     /gene="dusB"
                     /locus_tag="ACHKUC_004769"
     CDS             140..1105
                     /gene="dusB"
                     /locus_tag="ACHKUC_004769"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO6620855.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1131..1427
                     /gene="fis"
                     /locus_tag="ACHKUC_004770"
     CDS             1131..1427
                     /gene="fis"
                     /locus_tag="ACHKUC_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO6620856.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1513..2397
                     /gene="yhdJ"
                     /locus_tag="ACHKUC_004771"
     CDS             1513..2397
                     /gene="yhdJ"
                     /locus_tag="ACHKUC_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6620857.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVAPDLIAK"
     gene            2481..2660
                     /locus_tag="ACHKUC_004772"
     CDS             2481..2660
                     /locus_tag="ACHKUC_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO6620858.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2663..3325)
                     /gene="acrS"
                     /locus_tag="ACHKUC_004773"
     CDS             complement(2663..3325)
                     /gene="acrS"
                     /locus_tag="ACHKUC_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO6620859.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT          939 a          789 c          807 g          881 t
ORIGIN      
        1 taattatcgc gctacgctca ttttaaatgc aattctttga tccatctcag aggattggtc
       61 aaagtttggc ctttcatctc gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag
      121 tatggtcatt tcttaactca tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc
      181 agcgcccatg gctggcatta cagacagacc ttttcggacg ttgtgctacg agatgggagc
      241 cggattgaca gtatccgaga tgatgtcttc taacccacag gtttgggaaa gcgacaaatc
      301 tcgtttacgg atggtgcaca ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag
      361 cgatccgaaa gaaatggcag atgcagcacg tattaacgtg gaaagcggtg cccagattat
      421 tgatatcaat atgggttgcc cggctaaaaa agtgaatcgc aagctcgcag gttcagccct
      481 cttgcagtac ccggatgtcg ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt
      541 tcctgttacc ctgaaaattc gcactggctg ggcaccggaa caccgtaact gcgaagagat
      601 tgcccaactg gctgaagact gtggcattca ggctctgacc attcatggcc gtacacgcgc
      661 ctgtttgttc aatggagaag ctgagtacga cagtattcgt gcagttaagc agaaagtttc
      721 cattccggtt atcgcgaatg gtgacattac tgacccgctt aaagccagag ctgtgctcga
      781 ctatacaggg gcggatgccc tgatgatagg ccgcgcagct cagggaagac cctggatctt
      841 tcgggaaatc cagcattatc tggacactgg ggagttgctg cccccgctgc ctttggcaga
      901 ggttaagcgc ttgctttgcg cgcacgttcg ggaactgcat gacttttatg gtccggcaaa
      961 agggtaccga attgcacgta aacacgtttc ctggtatctc caggaacacg ctccaaatga
     1021 ccagtttcgg cgcacattca acgccattga ggatgccagc gaacagctgg aggcgttgga
     1081 ggcatacttc gaaaattttg cgtaaacaga aataaagagc tgacagaact atgttcgaac
     1141 aacgcgtaaa ttctgacgta ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc
     1201 aaaaacccct gcgtgactcg gttaaacagg cactgaagaa ctattttgct caactgaatg
     1261 gtcaggatgt gaatgacctc tatgagctgg tactggctga agtagaacag cccctgttgg
     1321 acatggtgat gcaatacacc cgtggtaacc agacccgtgc tgctctgatg atgggcatca
     1381 accgtggtac gctgcgtaaa aaattgaaaa aatacggcat gaactaattc aggttagcta
     1441 aatgcttgat taaaaaggcg ctactcggca tggggaagcg ccttttttat aggtgtcaca
     1501 aagggagtga ccatgagaac aggatgtgaa ccgacccgat ttggtaatga agcaaagacc
     1561 atcatccagg gtgatgcatt aaccgaactt aaaaagctcc ccgccgaaag tgtcgatctg
     1621 atctttgccg acccaccgta taacatcggt aaaaattttg atggtctgat cgaagcctgg
     1681 aaagaagatc tgtttatcga ctggctgttt gaagtgattg cagagtgcca tcgcgttctg
     1741 aaaaagcagg gcagcatgta catcatgaat agcacggaaa acatgccctt tatcgatctc
     1801 cagtgccgca agctttttac catcaaaagt cgcatcgtct ggtcatatga cagttctgga
     1861 gtgcaggcga aaaaacacta cggctccatg tacgaaccca tcctgatgat ggtgaaagac
     1921 gcaaagaact acacgttcaa cggtgatgct attctggtag aagccaaaac cggatcgcag
     1981 cgcgcgttga tcaattatcg caaaaatcct ccacagcctt acaatcatca aaaagtaccg
     2041 ggtaacgtct gggattttcc gcgcgtgcgt tatttgatgg atgaatatga aaaccacccg
     2101 acgcaaaaac cggaagcctt gctgaaacgc attattctcg cctcttccaa cccaggcgat
     2161 atcgttctcg acccgtttgc cggtagcttt actaccggtg ccgtagccgt cgccagcggg
     2221 cgaaaattca ttggtattga gatcaacagc gagtacatca aaatggggct tcgacggctg
     2281 gatgtcgcgt cgcattactc tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc
     2341 aacctgtcaa aacgaagccg gttaagcgaa gttgcccccg atctcattgc aaagtaaagg
     2401 aagtgtaagc ctggttttca gattattcat ttcgtgtata tttcctgcca gacttggtta
     2461 aacatgcaca ggctcaggta atgattcgca agtattggtg gctcgtcgtt ttcgctgtct
     2521 tcgtttttct gtttgatact ttactgatgc agtggattga actgctggca acagaaacag
     2581 acaaatgccg caatatgaac tcagttaatc cactaaaact ggttaactgt gacgaactga
     2641 attttcagga cagaatgtga atttatatga cacttaattc attcgtttta tgaattaatt
     2701 tcgttatgtt ttcatctggc atgaacattc ttaatacgtt atcgaccaga gcgggggctt
     2761 gtttataaag atcataaccc gccatattca ttaaccagtt ttgaacaatt ccgctgaagg
     2821 caccatcaat aataatcatc acaacatcta aatcgagatt atttgctaca caaccttgtt
     2881 gctgacacgc ctgcaatact tcgcggagag tctgcggatt aaagcccatc ttttcgcgta
     2941 tcactccctc ggccagcatc tcatcattaa attcacattt gtgatataag attttcagca
     3001 acgcctgctg gcggggaatt ttggcaatat attgcaaacc gacaatcaat ttttcacgca
     3061 attgctgaaa cgggtcatgc tctaacccag cggtcaagtg atcctggatt aactcccgca
     3121 atgaaggctg ttgcaaccac atctcattaa acagttgagt cttgttttcg aagtgccagt
     3181 agatagcgcc acgcgtaacg ttagcagcgt cggcaatgtc gttgagcgtc gtcttgctta
     3241 cgccatgctg cgcaaactgg gcgatggcag tttcaatcag ttcttgccgg gtcttcaggg
     3301 cttcagcttt ggttcttttt gccatgatta attattcagg aaataaatat attcgacaca
     3361 ggatgagaaa atagcgaagg ttaatctatc acctaatgtg tatttatacg agaggc
//
LOCUS       DAZFRG010000359         3410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFRG010000359 DAZFRG010000000
VERSION     DAZFRG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.361"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(123..842)
                     /gene="dcuR"
                     /locus_tag="ACHKUC_004774"
     CDS             complement(123..842)
                     /gene="dcuR"
                     /locus_tag="ACHKUC_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO6620860.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCRGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(839..2470)
                     /locus_tag="ACHKUC_004775"
     CDS             complement(839..2470)
                     /locus_tag="ACHKUC_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6620861.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2651..2881
                     /gene="yjdI"
                     /locus_tag="ACHKUC_004776"
     CDS             2651..2881
                     /gene="yjdI"
                     /locus_tag="ACHKUC_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO6620862.1"
                     /translation="MDRALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMQDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2893..3165
                     /locus_tag="ACHKUC_004777"
     CDS             2893..3165
                     /locus_tag="ACHKUC_004777"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6620863.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          879 a          780 c          853 g          898 t
ORIGIN      
        1 gcgatgatgg agggataatt atatttgatc tggcacaagt tttactgatg aacgatgtaa
       61 cttgtgccag ggggattttg cattacggta ataattatta cttacagata accagcgatc
      121 cgttattggc aatattgttt cagtagtgag tagtgttctg cctgaatacg gtaacggtaa
      181 accggacgcc ccgtgacgcc ataatggata ctggtgaaca atatgtggca gttgaccagc
      241 cagataaggt atttacggca ggaaacacgc gagatgttaa cctcgttagc cagctcgtcg
      301 gttgaaaact catagtcctg atgcgcgtca atccactggc acagcgtgcg taacgtctgc
      361 ggcgttaagc cttttggcaa gcgacgagga tcctgctcgt tggagctgct gccgtggatt
      421 agctgatcaa gctcggcctg gtcataatac tgatgttttt ccagcgccat tttcttttgc
      481 cgccagccgg tgagcgcctc ttcaaagcgg gaagcctgga agggtttgat caggtaatcc
      541 acaacaccgt aatgcagcga atctttaatg gttactgcat cggcagcgga ggagatgaca
      601 atcacatcac ttttgcaacg cgcgttatgc aggacaggca gtaaatcgag cccgttctct
      661 ttttgcatat agatatctag caatatcagg tcgataggcg tatcgctatt gaagataatc
      721 tctttggctt tctccagcgt cgaagccgtt ccacggcatt gaaagcccgg gatttgtgcc
      781 acgtatcggc gattcagctc cgcgaccatt gcgtcatcat cgataattaa tacattgatc
      841 atctgttcga cctctccccg tcccagggta tctggacaaa aaattgtgtg aaaatcccgg
      901 gttccgattc cacggcgatg ctgccgccga gattttctac ctgttgtttg acaagtgcta
      961 aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt gtcaaaaatg tgatcgattt
     1021 tgtcgggtgc gatccccggt ccatcatcgt taacttcgca gtgcagccag ccgtgacggt
     1081 agtgcaatgt tacgctaatt tcgccaccgg gttccggccc taatgcctcc agcgcgtttt
     1141 ctatcagatt tcccaacgtg gtaatcagcg tcgcgacctg atcctcacta ccgctgtctg
     1201 gcagctggct ttcactgttt aaaatcagcg tatggcctaa atcggtcgcg cggttaatct
     1261 tgctgattaa aaaaccagcg ataaccggag atttgatctt acccagcaga gagccaatct
     1321 cttcctgata gttattggct gttttgagaa tgtaatcttc caactgctta taactcttca
     1381 gatgcaataa tccgagaatc acatgcaatt tattcataaa ttcgtgggat cgttcacgaa
     1441 gtgcgtcagc atagttgacc agaccgtcga gtcgctgcat cagtttacgt acttcagttt
     1501 tgtccctgaa ggttgaaatg gcaccgatga taacgccatt actgcgcacc ggaacggtgt
     1561 tgatcagtaa tagtcggtcc ttaatcgtaa tctcttcgtc gcggcgtggg gttccgtcgc
     1621 gtaacacttc cgagacatct accacctgtg accacgagtg gcttagcgtc gacagtttct
     1681 catcgtcctg cgacttacgg taattcagca attcttgtgc ggcatcgttg atcagcgtga
     1741 cctcgccgcg atcgtccacg gcaacgacgc cttctttgat ggactgcaac atggcctggc
     1801 gttgctcaaa cagtgtggag atttcgtagg gttccaggcc gaaaaggatt tttttcagta
     1861 cgttaaccag aatgcatgtg ccgatcagtc cgaccagcat gccaaatagt accgaccaga
     1921 taatgctcca gcgactgtca ttgatctgtt gggtcacgcg gcttaactca aggccgatcg
     1981 ccaccacgcc aatttgttta tgattttcat cgtagatggg ggtaaatacg cgtaaagcct
     2041 gcgccagaaa accgcgattg atagcgacat tttcttcgcc attcagcgct ttaaggatgt
     2101 catcaccttt aaatggctga ccaatacgct gggcttcagg atgcgagtag cgaaggcttt
     2161 gcatatcggt aacgacaata aacagcagat cgttgcgttt gcgcacggct tccgcgatgg
     2221 cctggattcc actctcctgc ggttttttct gcaagccctg acggatttcc ggcgagtcgg
     2281 cgagggtacg cgctactgcc agtgccttgt tagctagccc gtctcgcgtc atatcactga
     2341 tttgcgagaa gtaaatcaga tgcaccacca atagcaccga gaacagtacc gcgctgacca
     2401 ttaagatcac agtggtactc aatttcatcg gacgtttgcg taacatacgg tagggcaatg
     2461 aatgtctcat cagcttcctt gtgtgacaaa tttcttaagc attatctctg atgaggcggg
     2521 taattcaaag ggagtaagaa tgattggcta tataggggaa gagactctgg caacggaaac
     2581 tgccagtgct gtatgaagat tccggggcta tgcttatagc gataatcata ctgatgagag
     2641 agggaaggtc atggatcggg cgctactgga cgggggttat cgctgttata ccggcgaaaa
     2701 tatcgatgtc tattttaaca ctgcgatatg tcagcattct ggcaattgcg tacgtggcaa
     2761 cggcaagtta tttaatctca aacgaaagcc gtggatcatg caggatgaag tcgacgttgc
     2821 cactgtggtt aaagtgattg atacgtgccc gagtggcgcg ctgaaatacc gtcataaata
     2881 agcgagggta aaatggaaat acgcgaaggc cacaataaat tttacattaa tgacgaacaa
     2941 ggcaagcaaa tcgctgaaat tgtctttgtg ccgaccggag agaatttagc gattatcgaa
     3001 cataccgatg tcgatgaaag cctgaaaggg caagggattg gtaaacagct ggttgcgaaa
     3061 gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc cactatgccc atttgcgaaa
     3121 cacgaatttg ataaaacacg ggagtatgat gatattcgca gttgatggga gagtacagag
     3181 tcacgatatt tttcatgctc tccgcggtgt gatgcaggag agcatctgaa gggtaggggg
     3241 atgcacaaag aatggtcaga gagagcgttt ttttgtccca agtcatcccc tttactgagc
     3301 aaaaaaaaaa gaatatctcc tatatgagaa tcatcattcg gggttaataa gttttgcgtc
     3361 cccagagcgt tcaatattga taggagtcat attatggaag gtaaaaacaa
//
LOCUS       DAZFRG010000360         3400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFRG010000360 DAZFRG010000000
VERSION     DAZFRG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.362"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..301)
                     /locus_tag="ACHKUC_004778"
     CDS             complement(<1..301)
                     /locus_tag="ACHKUC_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6620864.1"
                     /translation="MLIGQALDLISRYDSLRNPLTSLGDYLAPELISRCLAESGTVTL
                     HKRRLPLEMMVWCIVGMALERKEPLHQIANRLDIMLPGNRPFVAPSAVIQTRQRLG"
     gene            complement(427..3102)
                     /gene="adhE"
                     /locus_tag="ACHKUC_004779"
                     /gene_synonym="adhC"
     CDS             complement(427..3102)
                     /gene="adhE"
                     /locus_tag="ACHKUC_004779"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO6620865.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          846 a          821 c          871 g          862 t
ORIGIN      
        1 ttcccagacg ctggcgggtc tgaataacgg cactgggggc aacgaagggg cgattgcccg
       61 gcagcatgat gtccaggcga ttcgcaatct ggtgaagagg ttctttacgc tcaagcgcca
      121 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgtgt agcgttacag
      181 tacctgattc ggcaaggcaa cgagagatga gttcgggggc gaggtaatcc cccagagaag
      241 tcagtgggtt acgcagagaa tcgtaacggg atatcaaatc aagggcctgt ccaatgagca
      301 taaaaaaatc cggaaacgag tgagcgtttc cggattctta cacagccact ggagcggtca
      361 actgatcctt aactgactgg cattacccca gaaggggccg tttatgttgc cagacagcgc
      421 tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcag cttctttctt
      481 cgctgcagtt tcgccttcta cataatcacg accgtagtag gtatccagca gaatctgttt
      541 cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat cgaatgcatc
      601 ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag cttcacggat
      661 agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca gtttctcgat
      721 cttagctgca gtacggtcgc ccggtgcgct cagacccagg tggtcagcaa tttcagcata
      781 acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg tcgggttgtc
      841 attcgcgttg tagcgaataa cgttacaaat cagcagggcg tttgccagac cgtgcggaat
      901 atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga aggcgttcgc
      961 aaacgcgata cccgcgatag ttgctgcact gtgaacacgt tcacgcgcta ccggattttt
     1021 agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg cctgcagagc
     1081 ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg cgtgagttac
     1141 tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacaa ggttggcgtc
     1201 gacaatcgcc atatccgggg tcagtgcata gtctgccagc ggatatttct gaccagtagt
     1261 gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag tggtggtgac
     1321 agcgatcatt ttcgctttca cacccatttt cgggaacttg tagatacgtt tacggatatc
     1381 cataaagcgc agcgccagtt cttcgaagtg agtttccgga tgttcgtaca taacccacat
     1441 gatcttcgca gcgtccatcg gggaaccacc acccagcgcg ataatcacgt ctggtttgaa
     1501 ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtcag cttctacttc
     1561 gaagaagact tcagtttcaa cgcctgctgc tttcaatacg gaagtgatct gatcagcata
     1621 gccattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc catcagtaat
     1681 cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt tcggaagttt
     1741 gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca ggtgtttcgg
     1801 accaacgttt tcagagatgg agttaccacc ccaggaacca caacccagag tcagggaagg
     1861 tgcgagtttg aagttataca ggtcaccgat accaccctga gaagccgggg tgttaatcag
     1921 gatacgagcc gttttcattt tctgaccgaa gtaagaaacg cgagccggtt ggttatcctg
     1981 gtcagtgtac aggcaagagg tatgaccgat accgcccata gcaaccagtt tctctgcttt
     2041 ttctaccgcg tcttcgaaat ctttagcacg gtacattgcc agagtcgggg acagtttttc
     2101 atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa tcttggtgtt
     2161 ttctggtaca gagaagcctg ccagttcagc aattttatag gctggctgac caacgatagc
     2221 cgcgttcagc gcaccgtttt tcaggataac gtcctgaaca gctttcagct ctttaccctg
     2281 caacagatag ccgccgtggg ttgcgaaacg ttcacgtact gcgtcataaa cagagtcaac
     2341 aacaacaaca gactgttcag aagcacagat tacaccgttg tcgaaggttt tggacatcag
     2401 tacagatgca actgcacgtt tgatatcagc agtttcatcg ataacaaccg gagtgttgcc
     2461 cgcgcctacg ccgatagctg gtttaccgga gctgtatgcg gctttaacca tgcccggacc
     2521 accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag acagctcaac
     2581 agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga tagcagcctg
     2641 tagaacgata tcagccgctt tgttggtggc atcttttgca cgcgggtgcg gggagaagat
     2701 aatggcgtta cgggtcttca ggctgatcag cgatttgaag atagcagttg aagtcgggtt
     2761 agtggtcgga acgataccgc aaataatacc gatgggttca gcgatagtga tggtaccaaa
     2821 agtgtcgtct tcagacagaa caccacaggt tttttcatct ttataagcgt tgtagatata
     2881 ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc cggattcggc
     2941 aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg cggcgcggaa
     3001 gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt tttttacacg
     3061 ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct cctgataatg
     3121 ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg cggtgatgat
     3181 ttgcttaacc gatggaaatc aaaagcttac tcgcgctcac actcactgtg atttactaaa
     3241 agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc gtaagcaaga
     3301 ttactcactt ctgggtactg attacgtgat ccaaatcaaa attttgcaaa gctgacacct
     3361 ttcagcatgg cttttcgcca ttatagctaa cagttaataa
//
LOCUS       DAZFRG010000361         3366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFRG010000361 DAZFRG010000000
VERSION     DAZFRG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.363"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..143)
                     /locus_tag="ACHKUC_004780"
     CDS             complement(<1..143)
                     /locus_tag="ACHKUC_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6620866.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMG"
     gene            complement(158..520)
                     /locus_tag="ACHKUC_004781"
     CDS             complement(158..520)
                     /locus_tag="ACHKUC_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6620867.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMH"
     gene            complement(521..1579)
                     /locus_tag="ACHKUC_004782"
     CDS             complement(521..1579)
                     /locus_tag="ACHKUC_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6620868.1"
                     /translation="MRVLLRPVLVPELRLVVLKPGRESIQIFHNPRVLVEPEPKSMCG
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLCDQTSESLEQLAHQNLSAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADALPESVLCRSLGLPVEKIRSVYRESDIVPGEQTATSILKQR
                     TKNIALPLHVHQQQPPLQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(1581..1853)
                     /locus_tag="ACHKUC_004783"
     CDS             complement(1581..1853)
                     /locus_tag="ACHKUC_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620869.1"
                     /translation="MAHKTKFHNSDTDLAIRHERRSHAFKTDWYQHPPCTEEQAEWLI
                     HSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1800..1979
                     /locus_tag="ACHKUC_004784"
     CDS             1800..1979
                     /locus_tag="ACHKUC_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620870.1"
                     /translation="MPDGKVGIRIVKFGFVRHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(2021..2176)
                     /gene="hokD"
                     /locus_tag="ACHKUC_004785"
     CDS             complement(2021..2176)
                     /gene="hokD"
                     /locus_tag="ACHKUC_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6620871.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2785..3099)
                     /locus_tag="ACHKUC_004786"
     CDS             complement(2785..3099)
                     /locus_tag="ACHKUC_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620872.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKGVTDFQENLWVGDWKY
                     VPTKEDCNDRPNYEIQALFAAPPVPVTSEELVKAVHFYEQLKRENPPASGNLITDSQI
                     RQ"
     gene            complement(3096..3272)
                     /locus_tag="ACHKUC_004787"
     CDS             complement(3096..3272)
                     /locus_tag="ACHKUC_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620873.1"
                     /translation="MADFGSTKYNVSFEAWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
BASE COUNT          805 a          889 c          763 g          909 t
ORIGIN      
        1 cccatctcag cccgattctg tgtcatgccc tgcgcggcca tcacatcagt gatactcagc
       61 gcatcttttg acgttgaggc cgatgcatcg gtcaggccag gggattttgg ggagtagtat
      121 ttcggtaaat cttccagttt cattttttga cctgctctta atgcattatg gggtaaatct
      181 tcacccccag acgtccacca gatactggct gaccacgaac gatattgatt tcatcaaact
      241 gctcatcgtc cattaacact cccgcatgcg tcagcgcatc cagcggtgct ttcaggatat
      301 tgtccaggtc gcgacgacgc ttatccggtg gctctgcaat cacctttatc gccagccttc
      361 cggacaggtt taatttcagc cgctgctggc gaacaattag cgccacatca cggcgataac
      421 gctttccggc ctccgagatg aaatacgtat tgccatgacg tcgccagtag gtattcaccg
      481 tcggcgggta aggcaaaaca aattctatgc gttcagtcat tcatgctttc cacttcagga
      541 cacccgaatt tctcgcgtgc attaaaaaac gaatcagcaa caacagctgg ctgccgtgtt
      601 tttcttcaaa atcttttacc ccggcgtgca gttcgttatg acatttacgg cacagcggaa
      661 taacaaacaa atcatcagcc tttgttccca tacctcccag gccatgacca atgatgtgat
      721 gcggatcatc tgcctgatta ccgcacgtca tgcatttctg cgtttttacc cagcgcgtgt
      781 atacaggcat ctcttcccgt tgtggtttct ggcgctggag atactgagcc ggagactccg
      841 gatcaacggc gatgcttact aacgtctttt cctggagtgg gggctgttgc tggtggacgt
      901 gaagtggcag cgcaatattt tttgtgcgct gcttcagtat gctggtggca gtctgttctc
      961 ccggtacgat gtcactctca cggtatacgg agcggatttt ttccaccggt aatcccagtg
     1021 aacgacacaa tacagactcc ggaagcgcat cagccacctg attgcagacc gcccaccagg
     1081 ataattcggc cagcgataac tccctctcct gcgtaccgct gattgcgtga cggatgacgt
     1141 caatcatcca tgctgacaag ttttggtgag caagttgctc aagtgattct gaggtctggt
     1201 cacacaactg gttgtcgcag tgccagcaca acaccattgc gccggtacca taacggtgaa
     1261 tgacggtttc actgtggtga taatcaccgt gtggccactg gcaggattta atatggcgca
     1321 acagccagtc agacagtgca ccagcgccgc cagcagcacg aatcacacgc tcattgctga
     1381 aaaatggcag taatgattta tcctccgcca gcggctggcg aacggcaggg acgactccgg
     1441 atggcaggcc gcacatgctt ttcggttccg gctccaccag cactcgagga ttatgaaata
     1501 tctgtatgga ttcacggccc ggcttaagga ccaccagcct aagctcaggc accagaacag
     1561 gtctaagtaa tacccgcacg ttacctccag atccgttgct ggaaagtgcg ggacggacgt
     1621 ggtgggcgtt cggaataagg cagcctgaca gagattatcc agtgccgata gtcgagactg
     1681 agagctttct taacctcgaa cccgcgcctg cggtaagaat gaatcagcca ttcggcctgt
     1741 tcttcagtac atggtgggtg ttggtaccag tcggttttaa atgcgtgtga acgccgctca
     1801 tgccggatgg caaggtcggt atcagaattg tgaaatttgg ttttgtgcgc catctgtttt
     1861 ctctgctggc gcagcaggtg tcaggtgttc aggctgacgt gcgaattgta aaccagaatg
     1921 ccaggaaaaa acaaaacccg ccgaagcggg ttaagtgcgg gtgcgttgag gatgcctgac
     1981 acatcagagg tggcgaggga tttctccccc gccaggtctc ttactcctcg ggttcgtaag
     2041 ctgtgaagac agcgacctcc gtctggccgg ttcggattcg tacctcgcag aggtctttcc
     2101 tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg attaacatcg
     2161 ccttttgctg cttcatagcc tgcttctcct tgacctttcg gtccgtaaga ggctaatctc
     2221 tatgtgtcgc atagatatgg cctcagatta atgttaagcg tcttgcagga cgcgtaatgt
     2281 taactggggc ttttctctat ctgccttttg gtgttcatgc ctgagacaga tagcctcaag
     2341 cacccgcagt cattctactt aactaagatt tccccgcaaa ccgtttttgt ccggcacagt
     2401 aaatatccaa ctaaaccaat ggcgttcgct gtatttaccg ccagtattca atgcacatga
     2461 ccgccatgaa cccccctaaa aaaagggcat ttatatgtcc aaacattaat atcaaaacat
     2521 caactttttc catatacctt gctgtgaaga tgatgggcat acatgatgcg aacaaccaga
     2581 acgcaacaaa caaaaactgc aatgcgtttt tcattattcc ccctacaatc aatgtgcaat
     2641 aacatttaaa cacacctcaa tttggccgga catataaata tctaaaccag aaaaaatcac
     2701 ttacatagcg ttacaaactc tttagtctaa atattcatcg caaaatattc cccatactta
     2761 tcagcccgtt ctgcgccagg tagctcattg ccttatctgg gaatctgtaa tcaggtttcc
     2821 ggatgctggt ggattttcgc gttttagttg ttcataaaag tgcacagctt taaccagttc
     2881 ttctgatgtc accggaactg gcggggcagc gaataaggcc tgaatttcat agttcggcct
     2941 gtcgttgcaa tcctcttttg tcggtacata tttccagtca ccaacccaca gattctcctg
     3001 aaagtccgta acgccttttt tcacgtagcg atatcgccat gcaactggtt ttgcctgccc
     3061 tgccgtttca tgcccttcct gataattaat ctcgctcatt catcgcccca ctcatcacaa
     3121 tatgcttcga ccggagtttt tcctgcttca taatcatcac gccatgcttc agcatcagca
     3181 gcactgccac cacgtaactc tgcatagtcc attaacagtt catgccatgc ttcaaaactg
     3241 acgttgtatt tagttgaacc aaaatcagcc attttgctct tcctcttcgt cttttatttc
     3301 gtgatatgag taattgcagt agttaaagaa aatatctttt gcttcgtcat gtatttcatc
     3361 aggcgt
//
LOCUS       DAZFRG010000362         3352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFRG010000362 DAZFRG010000000
VERSION     DAZFRG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.364"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..766)
                     /locus_tag="ACHKUC_004788"
     CDS             complement(<1..766)
                     /locus_tag="ACHKUC_004788"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6620874.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGI"
     gene            884..1281
                     /locus_tag="ACHKUC_004789"
                     /pseudo
     CDS             884..1281
                     /locus_tag="ACHKUC_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1427..1852
                     /locus_tag="ACHKUC_004790"
     CDS             1427..1852
                     /locus_tag="ACHKUC_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6620875.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1849..2199
                     /gene="tnpB"
                     /locus_tag="ACHKUC_004791"
     CDS             1849..2199
                     /gene="tnpB"
                     /locus_tag="ACHKUC_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6620876.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            2230..3327
                     /locus_tag="ACHKUC_004792"
                     /pseudo
     CDS             2230..3327
                     /locus_tag="ACHKUC_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          891 a          858 c          791 g          812 t
ORIGIN      
        1 ttatacctgc caggacacca ctctgaataa gagtgtcaat aacctggtta caggctactt
       61 gcatttcctt cgccttaatc acatcattgt ttttgactgc ttcaaatatt tcaagataac
      121 gccaccccat tatattatat gtactaccga ttcccccatc tgcacctgca attaaccctg
      181 atgcaaatat ttcatcgtaa ccattatata acacaagctc tggatgattc cttttgattt
      241 gttccatctg gaataaatca ccggatgtct gttttaatgc acaaaccctg ggtattgtga
      301 caagctcatt tatctgatct aaagaaaaac gcactccgct aagagccggt atattataaa
      361 ctaccatcgg aagtccatct gcagaatcaa taatcttcct gtaatggata caatgctctt
      421 caaatgaaaa aggataataa aatggagtaa cagcagatat agcatgatat ccgtattttt
      481 ttgcagcttt tgcaagtacc tcgctttctg ccgtactgat tccgcccaca tgcgctataa
      541 gtgtcagtct tccatctgat tcatcggcta ctgtttcaag aattttttct cgctctgcaa
      601 cattctgaag aaatgcttct ccagtagagc ctccaacata caatccctta atattttgtt
      661 cgatattaaa acggatcagt tttcgaagac tctccatatc gatttgctgg gattggtcat
      721 aaggagtggg taatgctgat atgacccctt taaattcaca ttgcattttt aatcaccttc
      781 acttaactgc ataatgggat acctagtaca ccatcaagta caaagattat ccaacagaaa
      841 agaagcacaa tgtgaagatt atcaaaaaaa aaccatcaga aagctgtcat gccgctcagt
      901 taaggatcgg ttgatcgatc cagtgtccag ttactattct ctgcgtaacc ctctgcgact
      961 atcttgcccc ggaactgatt acccacagcc ctgaagagtc cgacactgtc acgctacgca
     1021 aacggcgtct tcctctggaa atgatggcct ggtgaattat cagtatggcc catgagcgta
     1081 aggaaccttc accagattgt gaaccggctg gacacatgct gccaggtagt cgcccctttg
     1141 tcgcgccgtg atccatgccc agcagggcca ggaagcgggg ccgtacgcca cgtgttcacg
     1201 taaacagcgc agccctgaca taacaccacg ctgtatccgc tctggtgcaa tatgaacctg
     1261 ctgaccatcg atggcgtgtt ctaactaaca caggacacgc cagaacacga cataaagctt
     1321 cactcgccag atacatggta agcgtaaact gaccgccgta tgtagccatt aagccagtat
     1381 tggtaacgta ggtgcccacc ttttcatcaa ctagtggaca cctgttatgg aacagaaaat
     1441 attatccgca gaaccccgca gaacattttc aaatgagttt aaacttcaaa tggttaaact
     1501 ggcttcacaa ctaggagcct ctgttgcccg tattgccagg gaacacgata tcaatgataa
     1561 cctcctgttc aaatggctca ggctctggca gaacgaaggg cgtatatcgc ggcgtcttcc
     1621 ggtaacaacc tcttctgacg ctggcgttga attattacct gttgagataa cgcctgatga
     1681 gcagaaagag cctatggcgg ctcttactcc gcttttatcc actccttcac aaagcacagt
     1741 cagtgccagt tcctgtaaag ttgagttccg ccacggtaac atgacgctgg aaaatccttc
     1801 accagagctg ctcacggtgt tgatccgtga actgaccggg agggggcgat gatctcgctc
     1861 ccatcagaca ctcgaatctc gctggttgcc ggcgttactg atatgcgtaa atccttcaac
     1921 ggtctgggtg aacaggtaca acatgtgctg gatgagaacc ccttctccgg tcacctgttc
     1981 atcttccgtg gccgacggag tgacatgatt aaaatcctct gggctgatgc tgatggtctg
     2041 tgcctgttca ccaaacgcct ggaggaaggc ctgtttatct ggcctgcggt gcgtgacggt
     2101 aaggtatcca ttacccgctc gcagctggca atgctcctcg ataagctgga ctggcgtcag
     2161 ccaaaaacat cccgtcttaa cgcactgaca atgttgtaaa aatgtcatgg ccggattata
     2221 aaaacggtca tgagtcggaa atacctcatt cgtatcactg agctggaaag gctgctctct
     2281 gagcaggctg aagccctccg tcagagagat ctgcaactga gtctggttga agagacggag
     2341 gccttcctgc gctctgcact ggcacgtgcc gaagaaaaga tcgaagagga ggagcgggaa
     2401 atagaatatc tgagggccca gatagaaaaa ctgcgccgga tgctgttcgg tacccgttct
     2461 gaaaaactgc agcgtgaggt tgaacaggct gaggcccaac tgaaacaacg cgaacaggaa
     2521 agcgatcgtt acagtgggcg tgaggatgac ccgcaggttc cccgccagtt gcgacagtca
     2581 cgtcatcgtc gtccgttacc agcacatctt ccccgtgaaa tacaccgcct ggagcctgaa
     2641 gaaagttgtt gcccggagtg tggcagtgag ctggattatc tgggtgaagt cagtgctgag
     2701 cagctggaac tggtgagtag cgccctgaaa gtgatccgca ctgttcgggt aaaaaaagcc
     2761 tgtacaaaat gtgactgtat agttgaagcg ccagcgccgt cccgcccgat agcgcgcggc
     2821 atcgcgggct ccggattact tgcccgcgtg ttaacgggaa aatactgcga acacctgcca
     2881 ctgtatcgtc agagtgaaat ctttgcccga cagggtgccg aactgagccg tgcattaatc
     2941 tccaactggg ttgacgcgtg ctgccagtta atgactccgc taaatgatgc cctgtaccgt
     3001 tacgtgatga acacccgcaa ggttcacact gacgacacac cggtaaaagt gctgacaccg
     3061 ggcagaaaaa aggcaaaaac aggacgcatc tggacgtatg tccgggatga ccggaatgcg
     3121 ggctcatcag agccaccggc ggtctggttc gcctactcac cagacaggca gggaaaacat
     3181 ccggtacaac accttcgtcc cttccggggt atcctgcagg cggatgcatt cagcggttac
     3241 gatcggctgt tcagtgccaa acgtgaaggt ggtgcacaga cagaagttgc gtgctgggtc
     3301 catgcccggc gaaaaataca cgatgtatga acgacatctc ttctgacgac at
//
LOCUS       DAZFRG010000363         3338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFRG010000363 DAZFRG010000000
VERSION     DAZFRG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.365"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..487)
                     /locus_tag="ACHKUC_004793"
     CDS             complement(29..487)
                     /locus_tag="ACHKUC_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO6620877.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMHRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(542..1393)
                     /gene="manZ"
                     /locus_tag="ACHKUC_004794"
     CDS             complement(542..1393)
                     /gene="manZ"
                     /locus_tag="ACHKUC_004794"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO6620878.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1406..2206)
                     /gene="manY"
                     /locus_tag="ACHKUC_004795"
     CDS             complement(1406..2206)
                     /gene="manY"
                     /locus_tag="ACHKUC_004795"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO6620879.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2269..3240)
                     /gene="manX"
                     /locus_tag="ACHKUC_004796"
     CDS             complement(2269..3240)
                     /gene="manX"
                     /locus_tag="ACHKUC_004796"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO6620880.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT          888 a          861 c          796 g          793 t
ORIGIN      
        1 taaacatagc tttggctaaa ttcataactt attgagttga aacgagaagc ttataaattt
       61 tttccaaatc gtcgatattt cgaacgcgga ttaacagccg acgctgttct aattgcatca
      121 ccaacacgcc atcttccgac aagttcatcg ctttgattcg gctatattca atccagacat
      181 tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg aacgcgaatc cagaatatat
      241 aaaaacccat cagagccagt gcacttaata accatgttgt tataagcgcg ccatgattcg
      301 tgacgttgtt atagataaga atgacaatca atccgacgaa gataacgcta tcgatgcgac
      361 cacgccggag caaaggaatt gccagcaggg tggggccgtt acggcggtgc atgatgaact
      421 gatcgtagat cgcgaaggcc agaagtgcgg cgatgaaaag aatcagtacc aggtccgtga
      481 ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt
      541 cttacagtcc cagcaggccg caagcgtaac cagcgatacc gatgacgaag aagccaacga
      601 tgatccacag cgggttaact tttttgcgca acagccacat acaagcaaag gtcagcagca
      661 gtggcacaag gcctggcatt aactggtcca gaatagtctg gacagtggta acgtgttctt
      721 tgcccgtctg gtcagtaatg cgagagacca ccagcgggat gttgacatgt gtccacttat
      781 taaccaatgc ccccatgaca aacaggccga ggatagacgc cccttccgtc agtttttgca
      841 ggaagccacc acccatatct ttaacgatat cgataccttt ggagtaacca tacgctacgc
      901 cgtagtaacg ggttgccaga cgcaccaggt taaacaggat gaagaacagc agcggaccta
      961 acaggctgcc gctcatcgcg ataccagccc ccagcgctgc aaataccgga cgtaccgttc
     1021 cccagaagat cgggtcgcct acaccagcca gcggccccat caaaccgact ttgataccgt
     1081 tgatagcacc gtcatcgatc tctgcgccat tagcacgctg ttcttccagc gccagggtta
     1141 cgccgagaat cggcgcagcc acgaacggct gggtgttaaa gaactccagg tgacggcgaa
     1201 tagcttgttt acgagcttcg ttgttctcag ggtagaggcg acgaattgcc ggtaccatag
     1261 agaagcagaa acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg aagaggttag
     1321 aacgcaggaa gacgccacga atatcacttt gagtgagttt tttctcggtg gtagtttgag
     1381 ttgtatcaac catttcgctc acctgttagt ccagttcgtt atcgagatcg ttgttaccag
     1441 ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg atgtagagca
     1501 ctgccataac agtaccaatc acacccagag caaccaggtt aaagttggtg aatgctgcgg
     1561 ttacgaagcc gaggtagaag aacggcatca ggtagccagc acgcatcatg ttgataacca
     1621 tcgcataacc aaccaccacg atcatgccac cggcgatatt cagaccattg gtcaccactt
     1681 ccggaatcgc attcagcatg ttctgtactt cgctggtacc aacagacaac gcaacgatga
     1741 cggccggaat agccacacgc attgcttgca ggaacagaga agaaacgtgg atccaggaaa
     1801 tcgctgtcag gttgccgtta tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa
     1861 tagtacgaac gatgatggtc agtacctggc ccgcagcggc cagagggatt gccagtgcga
     1921 tacctgcacc aatgctctga tgacctgcga taaccagaat agtagaaatg atggaagcca
     1981 gagcggcgtc aggcgcaact gcagcaccga tgttcatcca gcccagcgcg atcatttcca
     2041 gcgtaccacc gataataata ccggttttca tatcgccaag aacgataccc accagggtac
     2101 acgcgattag cggacggtga aactgaaatt catcgaggat tgatcccata cctgcgatac
     2161 aggctacgat aaataccagc acaatttgaa gagtggtaat ctccattgta cttctcctgt
     2221 ttacgtaaga cttaagtgtg aaaactgagt gataatcaac acaatacgtt acttatcgat
     2281 tttgctgatc agatccatca ttttcagttt cggatcggtg gaaaccttac ggacttccag
     2341 ctcaataccg cgcgcattca gtttcttgaa cgcctcgata tctttttcat caaccgaaac
     2401 cgcgttattc acctgggttt taccctgacg gaatgccata ccaccgacgt taacagaggt
     2461 gattttcacg ccgccttcaa cgagacgctc tacatctgtt gggttggtaa ataacagcat
     2521 tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga atcattttgg caacatcaac
     2581 tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc
     2641 cgcagccact tcatcactaa caacaataat acgggagaca ttggtttctt tggtccagcg
     2701 ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacga gcaaggccaa taaccatgta
     2761 gtcgtttggt cccattggtt ttgccggagt tggagccgct tttggtgctg cggcagcggg
     2821 tgctggcgcg gctttttcaa ccggtttggc tttcagtgct ttcacgcctt cacggcctgt
     2881 ttctactgcc agcgccacca gttcatcaaa gcttgggtca tcatcacggg ccattaacgt
     2941 ttccacgagc attggaatgt taacgcctgc aatgacttca taatgctctt tgtcgacgac
     3001 aatgcggctg gcagcattga acgggctgcc tccccatgta tcaacgagaa acagcacgcc
     3061 tttagtggtg tcgagttttg ccaactgagc gttgtacttt tcaatcagcg tttcggcatt
     3121 ttcacctgga acgaaatcga tccagccgac gttttcctgc tcgcctaaca gcatttctgc
     3181 cgttttaagc aactgctctg cagcccaacc atgtgtgcct ataacaatag caatggtcac
     3241 ttgctacctc ctttattatc gttaacacct caacgtgcca gatgtatttt tgaatcgtcg
     3301 tccacaatcg aatcgattca gataagggct acaaccaa
//
LOCUS       DAZFRG010000364         3335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFRG010000364 DAZFRG010000000
VERSION     DAZFRG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.366"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..1233
                     /locus_tag="ACHKUC_004797"
     CDS             16..1233
                     /locus_tag="ACHKUC_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO6620881.1"
                     /translation="MSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTTYTIVYNSDS
                     DTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVYDREFHDLMN
                     SLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEYGRVATSAES
                     IKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIAKDVEEQKEG
                     LAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGATKTIAVNDG
                     LVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMK
                     RAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASD
                     GDVSYNVGVNFEF"
     gene            1320..1793
                     /locus_tag="ACHKUC_004798"
     CDS             1320..1793
                     /locus_tag="ACHKUC_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620882.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            2164..>3335
                     /locus_tag="ACHKUC_004799"
     CDS             2164..>3335
                     /locus_tag="ACHKUC_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6620883.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRP"
BASE COUNT          854 a          634 c         1004 g          843 t
ORIGIN      
        1 tttacaaaga cacttctgtc atcttctctg gttgtaggtc tgttgagtgt tagttctttt
       61 acaagtgcaa gtgaatatag ctacactgcc ttggatattg tcggtagaca cggaactact
      121 tacaccattg tttataatag tgattctgac accgcttatg ttaaatggaa aacagtacct
      181 gccggtgctg aagggattga aagtggaagt attgtacaag gattatcaaa aattaataga
      241 gatgggactg tagaagttac taccaaggat ggcactagta catatcgtgt gtatgaccgg
      301 gagtttcatg acctaatgaa ttcactggtc aagcagaaaa gagaaggttc tcttggaaag
      361 aacgagggta ctaaaacaga gcttgtatct gatgctgata ttcggaatct ttttggcaga
      421 attgctgcga tctctgaaat ggaaactaat gttggtctgg atgaatatgg gcgagtggca
      481 actagtgcag agagtattaa agaacgcgtg tctttgcaga gttatctggc tacagaggct
      541 gcaaatagcg caattggcct agataaaaac ggtaagctaa ctctgaatga gacagatatt
      601 gtaacgccgt tacgtatcaa tgacggcgtt gtcactatag cgaaagatgt ggaagaacag
      661 aaagaagggc tggcgatcca gaagaaagga ctgtctgagc tttcatcccg cacagatatc
      721 attgatgcag caacggctgc gaatacgaat aaaattgatt cccgaaccac cgttggtgtg
      781 aatgcggacg gaaccttgac cagcgctgat ggcgctacaa aaacgattgc tgttaatgac
      841 ggtctcgttg ctctgtccgg cagaacagac cgtattgatg ctactgttgg tagcgttgac
      901 cgccgggtta ctaaaaatac acaggctatt cagtccaata ctcgccagtt gcaggaacat
      961 aatgcacgcc tgaacagcca gcagcgtcaa attcgtgaga atcacgaaga gatgaagcgt
     1021 gcagcagcac agagtgcagc gctggcgggg cttttccagc cgtacagtgt ggggaaattc
     1081 aacgccactg ctgccctggg ggggtacagt gataaacagg ctgttgccgt gggtgttggt
     1141 taccgtttca atgagcagac agcagcgaaa gcaggtgtgg cagcgagtga tggcgatgtg
     1201 tcctacaacg taggtgtgaa ctttgagttt taattgctga cagtgacatg aagcatgacg
     1261 gcaggcgtgg agttttgcct gccgtttttt ctcatgtgag catgaaacag gtgacagcga
     1321 tgaaagtgat tctggcgacc cgcaaccgtt atctggagta tggcctgcag cagatgcttg
     1381 aggggtacag gataatactg gcgagggagt tttttacacc agagaacagg aagagtgtac
     1441 cggcacatga tgagtcatgg gtgataatct gtgatgccct ccttggcagg ctgatgtgct
     1501 gcatgtttca gggacgccgt tatcttcaga ttgatgcgga agatgttacg gggcgcctgg
     1561 agacgtacag gaaaatccgg aatggtgagt gggtacataa tacgtatgcg cgacctctga
     1621 cgatgtccga gatggtggtg atgtttggat acgtgtaccg ggagtcaaag ccctgccatc
     1681 ttgcccgtga aatggggata aacacgaaga cggtgaacac ctttctgtat atgggacttg
     1741 gtaaaaatgg tcttaagtac aggagtgtga agcacctggt tggacgtgcg tagccggaat
     1801 gcatattcgt gagtcgggta atgagagtgg aacggtggca gtgagaatgg aacagaaaaa
     1861 aatcagatgc ggaaagcgga aatacacagc gggtgcgatg tgcttcatgc tgtttgtatg
     1921 gctggggggt gtggtgacat tctgtgtgct ggccgctctg ataatgaggt gaaaacggaa
     1981 gagagtttac ttccttaatt cgaactttct gataactgag aacttgttaa atgcctgtgg
     2041 gtttatgtat ttaaatcaat tggttaatgg tgattttatc tttatcagtc aaagttcgtt
     2101 ttagtatgat gattgtgccc gctgtttatt gttgtccggg agaaaataca gggaatccag
     2161 gggatgaaca aaatatactc actcagagtt aatgacagaa aagaactggt tgctgtaccg
     2221 gaaacggtgg ggggcaggaa aaagtcgtcc ggcattcccg gtgcggggat gctttcccgt
     2281 cttcttctgg cctcaggagc cgttgccggc gttctgtttt cttctcctgc gctggcatcc
     2341 gttgtcggga atactctgcc atggcagacc tacagggatt ttgctgaaaa caaaggggcg
     2401 tttcatgccg gggccacaaa tattcctctt tatggtaaga atggggctgt cagtgggcgg
     2461 ctggacaagg ctccgatgat ggatttcagt gttgtggacc agatactggg ggtggcaaca
     2521 ctgatttctc cgcaatatgt ggcgggggtt aagcataacg gcagctacaa tacggtgcgt
     2581 tttggttatg cggacgatac gacatatcgc cttgttgacc gtaatgagca ctggcgtgat
     2641 tttcatactc cccggctgaa caaactggtg acagaagttg ccccggtgag cgtcacggat
     2701 gccggaaccg gcaagggggt ataccagaac cgcagtcgtt atccggtgtt ttatcgtatg
     2761 gggagtggaa cgcagtacac cggagctgct tccggtgcgc tgaccagaat agcggatgct
     2821 tacgtctgga ggactggcgg gacggtggga agtccggtca tttctgactg gagtctggtg
     2881 tcaaacccgg gaaatcttta ccagggtgtt aatggtccgc tggcgtctta cgggacatca
     2941 ggagacagtg gctcaccgct ttttgcctgg gatgcggtga agaagcagtg ggtatttgtt
     3001 gctgtacttc atggttatgc cggtgagaac gggaagacga actggtttac ggtgattcct
     3061 gcaggggatg tgaataacac gataaagcag gacagttcag gcaccgtcgt gccggcagtt
     3121 gccggtggtg acattgtgtg gaattacagt aagggaagcg gagagggaac cctttctcag
     3181 gatgggaagg tatggaagat gaacggcttc aggggaggga gtctgaatga cgggaaagat
     3241 atcacctttg ggggtaaggg cacggttgtt ctgaaggatg atgttgtcca gggggcgggt
     3301 tcgctcacgt ttaatggtga ttatacggtc agacc
//
LOCUS       DAZFRG010000365         3329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFRG010000365 DAZFRG010000000
VERSION     DAZFRG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.367"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            513..2483
                     /locus_tag="ACHKUC_004800"
     CDS             513..2483
                     /locus_tag="ACHKUC_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6620884.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTSWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTEKTDALLSVIIRKRGSIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYRNSTTEPGNPTLTHGDSGLRDRKTAQNDM
                     QFWYQYAPADNSLINVKSTLYLSDITVKTNGHNKTAEWRNNRTSGVNVVNRSHSLIFP
                     GAHQLSYGAEYYRQQQKPKGTATLYPEGHIDFTSLYFQDEMTMESYPVNIIFGSRYDR
                     YNSFNARAGELNAERLSPRAAMSVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKSENNTTREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDIQGNYQSDYVDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHRGVYAGWRASLSAAATHVKKGDPCQDGYAIHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVNLYTRYQW"
     gene            2502..3293
                     /locus_tag="ACHKUC_004801"
     CDS             2502..3293
                     /locus_tag="ACHKUC_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO6620885.1"
                     /translation="MMKNTGYILALCLAASGQTLAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRDFFETPRLYNGKETITLKPTSTDYVYKTDSAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVTYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHATEHKHSAHKAELTFVSN
                     EDGIITIPSLHAGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT          960 a          754 c          769 g          846 t
ORIGIN      
        1 cttattcttt ggccatttta gcactgctga ctgacttgtc tgaaaagcat ggcattatgc
       61 cttaataacc gtaagaatgt tactcctctg agtacaagaa atctattata ggtcaataaa
      121 cattgacaga tattgtccta ctacaataaa tgattctttt tcggagatat attaagacat
      181 actaacagtt taaatatctt tgagtaaaca agccgtcatt tcacatgaga gcatccgtgc
      241 ccaactggtt tctatatatg atgatgaatg tatgagttaa cgctcattcg tcatagaaca
      301 gatttgcagc ttctcctgta cttgaataaa cgttaactaa ttccgtattg ctggttcttt
      361 atcggtagaa aaatcaatat ccatagcatc acattgcaga gcgaccgggt attagagtcg
      421 ctattcactg gttatcactg aacgctgcgt aatagtggtt gcacaaacaa aatgttatgt
      481 tataacattg ctgtattggc atatattttc gaatgtatat gaacgtaatc agaactgtca
      541 tttgtacatt aatcatactt ccggtggggt tacaggcagc gacaagtcat tcttctatgg
      601 ctaaagatac aatcaccgtt gtcgcgacag gaaatcagaa cacggtattt gaaacgccgt
      661 cgatggtcag tgtcgtcacg aatgacacat cgtggagtca gaatgcggtt acatcggccg
      721 ggatgctgaa aggtgttgcc ggtctcagcc agactggtgc aggacggacc aatgggcaga
      781 cctttaatct acgcggctat gacaaaagcg gggtacttgt tcttgttgac ggcgtccgcc
      841 aactcagtga catggcaaaa agcagtggca cttatctgga tccggcactc gtcaaacgta
      901 tcgaagttgt ccgcgggcca aactccagtc tgtacggcag tggcgggctg ggaggtgtag
      961 tggacttcag aactgccgat gcagcagatt ttcttccccc cggagagaca aacggtttaa
     1021 gtctgtgggg aaatatcgcc agtggtgacc acagcacagg ctcggggctt acctggttcg
     1081 gtaaaaccga aaaaacagat gcgctccttt ctgtcattat acgtaaaaga ggaagtatct
     1141 atcagagtga tggtgagcgt gcacctaaca aggaaaaacc tgcagccctg tttgcgaaag
     1201 gctctgtcag tattacagac agtaacaaag caggcgccag cttacgcctc taccggaata
     1261 gcaccactga accgggcaat ccaactctga cacatggtga cagtggcctg cgtgacagaa
     1321 aaacagcaca gaatgacatg cagttctggt accagtacgc ccctgcagat aacagtctca
     1381 tcaatgtaaa atcgacacta tatctcagtg atatcaccgt caagacaaac ggccacaaca
     1441 aaacagcaga atggagaaac aacagaacct ccggtgttaa tgttgtcaac aggagtcatt
     1501 ctctgatttt tccgggagcc catcagttaa gttatggcgc tgaatattac cgtcagcagc
     1561 agaagccgaa aggcactgcc acactatatc cggaaggaca cattgacttt acatcattgt
     1621 atttccagga tgaaatgaca atggaaagct acccggttaa cattatcttc ggttcccgtt
     1681 atgaccgata caatagcttc aatgcccgtg ccggagaact aaatgccgaa cgtctgtccc
     1741 ccagagcagc gatgtctgtc tcaccgacag actggctgat gatgtacggc tccatatcct
     1801 ctgcattccg ggcccccaca atggcagaaa tgtacaggga tgatgtacat ttttaccgca
     1861 agggcaaacc taattactgg gttcctaacc ttaatctaaa atctgaaaat aacaccaccc
     1921 gtgagattgg cgcaggtatt caactggacg gcctgcttac agacaatgac cgactgcagt
     1981 tgaaaggcgg atatttcgga acggatgcca gaaactatat tgccacgcgc gtggatatga
     2041 aacggatgcg ttcttattct tataatgtat cccgggcccg tatctgggga tgggatattc
     2101 agggaaatta ccagtctgat tatgttgact ggatgctttc ttataaccgg acggaaagta
     2161 tggatgccag cagcagggaa tggctgggct ccggcaatcc tgacacactt atcagtgaca
     2221 tcagcatacc tgttggtcat agaggcgttt atgcaggatg gcgtgcttca ctttcggcag
     2281 cagccacgca tgtgaaaaaa ggcgacccct gtcaggatgg ctatgccata cattcctttt
     2341 cactgtctta taagccagta agtgttaaag gctttgaggc gtcggtaact ctggataatg
     2401 ccttcaacaa gcttgccatg aatggaaaag gtgtgccgct ttcaggcaga accgtcaatc
     2461 tttatacccg ttatcagtgg taacatctca ggaaataaat catgatgaaa aatacaggct
     2521 atatcttagc tctttgtctg gcagcatcgg gccagaccct ggcccacgat gtctggatta
     2581 ccggtaaaca ggcagagaac aacgttactg cagagattgg ttatggccat aatttcccct
     2641 caaagggcac gattcctgac agaagggatt tctttgaaac tccccgactt tataacggga
     2701 aagagacaat aacgctgaag ccaacgtcca cggattatgt ctataaaact gactctgcgg
     2761 gcaacggtaa tagctacgtt ctgtcaacga aaatgaaacc gggatactgg tcgagaacct
     2821 catcaggatg gaagcctgtc agccgggagg gcagaaatga tgtgacatac tgtgagtttg
     2881 tgactaaata tgcaaaatct tttattcctg gtgaacagca gatgccagca caactctatc
     2941 agtctccaac aggggatgag cttgaaatca ttccattatc cgatatcagt cgtttcggtg
     3001 aagatgtgaa gctgaaagtt ctgtataaaa cgtcccctct tgccggagcc actctggagc
     3061 ttgactctgt cagttatctg aaatcatccc gtcacactca tgcaacagag cataaacatt
     3121 cggctcataa agcagaactt acctttgtaa gtaatgagga cggtatcatc actatacctt
     3181 cccttcatgc cgggcaatgg ctggctaaag tcaaaaataa gaaagtattt ccggacaaga
     3241 gcctgtgtga tgaaactgtc gatgtggcaa ccttaagctt ctcccgaaat taataatact
     3301 attccctgct ctgaaattta ttggcaggg
//
LOCUS       DAZFRG010000366         3316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFRG010000366 DAZFRG010000000
VERSION     DAZFRG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.368"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            318..1634
                     /gene="shiA"
                     /locus_tag="ACHKUC_004802"
     CDS             318..1634
                     /gene="shiA"
                     /locus_tag="ACHKUC_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO6620886.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1736..3190
                     /gene="amn"
                     /locus_tag="ACHKUC_004803"
     CDS             1736..3190
                     /gene="amn"
                     /locus_tag="ACHKUC_004803"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO6620887.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGRGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          758 a          806 c          806 g          946 t
ORIGIN      
        1 cataactaac ctcgcctccc tactggttat aatgcaatgc agtctatcag aatcatcagg
       61 gtgcaatttt gtgcatatgc ggacttttat gtttcatatc tcgaacctgt gggccctctg
      121 cttaatcctt aaacaacacc agcaactcct gcgctttcat cttccatcga atttttcatg
      181 ttgccgctaa tcagccataa aatcatttgc agatgcgctc tgtcgaggta gtctcataag
      241 gttcgtttat agatcgacgg caatgtgagt taccttttcc atactaatta taaaaagaca
      301 gtacaaacag gatcattatg gactccacgc tcatctccac tcgtcccgat gaagggacgc
      361 tttcgttaag tcgcgcccga cgagctgcgt taggcagttt cgctggtgcc gtcgtcgact
      421 ggtatgattt tttactctat ggcattaccg ccgcactggt gtttaatcgc gagtttttcc
      481 cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc cacctttggt gtcggcttcc
      541 ttttccgacc gctcggcggt gtcattttcg gtcactttgg tgaccggctg ggacgtaagc
      601 gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac agccttgatt ggtattcttc
      661 cttcattctc gaccattggg tggtgggcac ctattttgct ggtgacactg cgtgccattc
      721 agggatttgc cgtcggcggc gaatggggag gcgcagcgtt gctttccgtt gaaagtgcgc
      781 cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt tggctacgga gtaggtttac
      841 tgctttcaac cggactggtt tcattgatca gtatgatgac gactgacgaa cagtttttaa
      901 gctggggctg gcgcattcct ttcctgttta gcatcgtact ggtactggga gcattgtggg
      961 tgcgcaatgg catggaggag tccgcggaat ttgaacaaca gcaacataat caagcggccg
     1021 cgaaaaaacg catcccggtt atcgaagctc tgttacgaca tcccggtgct ttcctgaaga
     1081 ttattgcact acgactgtgc gagttgctga cgatgtacat tgttactgcc tttgcactta
     1141 attattcaac ccagaatatg gggttaccgc gcgaactttt ccttaatatt ggtttgctgg
     1201 tcggtggatt aagctgcctg acaattccct gttttgcctg gcttgccgat cgttttggtc
     1261 ggcgcagggt ttatatcaca ggcgcgttga tcggaacgtt gagcgcattt cctttcttta
     1321 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
     1381 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
     1441 gttatcgcta cagtggtgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
     1501 ttacgccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
     1561 ctatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
     1621 atcaacgcgc ttaatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
     1681 cattcctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
     1741 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
     1801 gcaatctgta gttgctttac gcaacgccat tggcaaatat attacaagtg gcgaattacc
     1861 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
     1921 cggtagcaca actaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
     1981 ttacaccacc acgattactc gccctactct ctttcgttcg tatctcaatg aacaacttac
     2041 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
     2101 ttatccttat gtcatcgatg gctctgaatt aacacttgat cgctcaatga gcgctggttt
     2161 aactcgctac ttcccgacaa cagaactggc gcaaatcggc gatgaaactg cagacggcat
     2221 ttatcatcca acggaattct ccccgttatc gcattttgat gctcgccggg ttgatttttc
     2281 cctcgcacgg ttgcgccatt ataccggtac gccagtagaa cattttcaac cgttcgtctt
     2341 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     2401 cgatcctgat agtccctaca ttgccctttc ttgtgctggc gggaactgga tcactgccga
     2461 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     2521 atggcattta attaccgctg atggtcgggg tattactctg gtgaatattg gcgtgggacc
     2581 gtccaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     2641 gattggtcac tgtggcggat tacgtgaaag ccaggccatt ggagattatg tccttgcaca
     2701 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     2761 gagcattgct gaagtgcaac gtgcgcttta tgacgctacc aagttagtca gcggcaggcc
     2821 tggtgaggaa gtcaaacagc ggctgcgtac aggtactgtg gtaaccacag atgacaggaa
     2881 ctgggagtta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     2941 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     3001 actactgtgt gtttcagata aaccgttaca tggtgagatt aagcttcccg gccaggctaa
     3061 ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt ggcattcggg cgatcgattt
     3121 gctgcgcgca gaaggcgacc gactgcattc acgtaaatta cgaaccttta atgagccgcc
     3181 gttccgataa taagttatga accttcttat cagaaataga tgtgaaggat taagtaagac
     3241 atctggcaaa tagcctgcaa taaacgcggg ctgtttgctt ccttgggcag atgcgagttt
     3301 tattatcgtc ttaatg
//
LOCUS       DAZFRG010000367         3310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFRG010000367 DAZFRG010000000
VERSION     DAZFRG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.369"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1988
                     /locus_tag="ACHKUC_004804"
     CDS             <1..1988
                     /locus_tag="ACHKUC_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO6620888.1"
                     /translation="GVGAFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRD
                     TFNTEQVEVIKGPSGTDYGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDV
                     NQVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQH
                     NTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSITDTATMRFEHD
                     INDNATIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKIL
                     TNQTNLTSTFYTGAIGHDLSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLT
                     RNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITC
                     PAGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQ
                     SGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQ
                     YGKKRVEGYEISVAGNITPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTL
                     WSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQL
                     NVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANMHF"
     gene            2204..2881
                     /gene="ybiX"
                     /locus_tag="ACHKUC_004805"
     CDS             2204..2881
                     /gene="ybiX"
                     /locus_tag="ACHKUC_004805"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO6620889.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            2955..3221
                     /gene="ybiI"
                     /locus_tag="ACHKUC_004806"
     CDS             2955..3221
                     /gene="ybiI"
                     /locus_tag="ACHKUC_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO6620890.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT          821 a          896 c          862 g          731 t
ORIGIN      
        1 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgcgg tgccgatacc tctaacagta tttatatcga tggcattcgc gatatcggca
      121 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt aattaaaggg ccgtccggca
      181 ccgactacgg gcgcagcgca ccgacaggct cgatcaatat gatcagcaaa cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacca ctgcggtgcg cctgaatgta atgggcgaaa
      361 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
      481 acaacacgcc agacggcggc attccgacta tcggtttacc gggttattct gccccatctg
      541 cgggaacagc agccctgaat cattccggaa aagttgatac tcataacttt tacggcacag
      601 attccgatta cgacgattcg atcaccgaca ccgctaccat gcgttttgag cacgacatca
      661 acgataacgc caccattcgc aacactacgc gttggtcgcg cgtaaagcag gattacctga
      721 tgacggcgat tatgggtgga gcgtcgaata ttacccagcc caccagcgat gtgaatagct
      781 ggacgtggtc acgcacggcg aataccaaag atgtgagtaa caaaattctc accaaccaga
      841 ccaacctgac ctcgacattc tataccggcg ctatcggtca tgatctcagt accggcgtgg
      901 aatttacccg cgaaacacag actaactatg gcgttaatcc ggtgacgtta ccggcggtaa
      961 atatttatca tcctgacagc agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg
     1021 ccaatggtca gacggatacc ttcgcaattt atgccttcga tacgctgcaa atcacccgtg
     1081 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcta
     1141 ccgcctgcgg cggcagcggg cgcggtgcca tcacctgccc tgctggcgtg gcaaaaggtt
     1201 ctccggtcac caccgtcgac accgccaagt cgggcaatct ggtgaactgg aaagccgggg
     1261 cgctgtatca cctgacggaa aacggcaatg tctatattaa ctatgccgtt tcccagcagc
     1321 cgccgggcgg caacaacttc gcccttgcgc agtctggcag cggtaacagt gccaaccgca
     1381 ccgattttaa accacaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
     1441 ataaacgcct gctacttacc gccgcgctgt tccgtactga tattgaaaat gaagttgagc
     1501 aaaatgatga cgggacttat tcgcaatatg gtaagaaacg tgtcgaaggc tacgagatat
     1561 ccgtggccgg gaatatcacc cccgcatggc agatgattgg cggctatacc cagcaaaaag
     1621 caactatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccgtataccc
     1681 cggaacacgc cttcacctta tggagccagt atcaggcaac cgatgatatt tctgttggtg
     1741 cgggcgcacg gtatatcggc agtatgcata aaggttcaga cggcgcggtg ggaacgccag
     1801 cgtttaccga aggttattgg gtcgccgacg ccaaactggg gtatcgggtt aatcgcaatc
     1861 tcgacttcca gctaaacgtc tacaacctgt ttgataccga ttacgtcgcc tcaatcaata
     1921 agagcggcta ccgttatcac ccgggcgagc caagaacctt cttgctcaca gctaatatgc
     1981 atttctgatt cagatgtggg gcgcaaggct cactaatgtt gatggcgaga gcaggactgc
     2041 ctgatgcgct gcgcttatca ggcctacaag gttgtgcaat acactgaatt cacatcattt
     2101 ggtaggccgg ataaggcgtt cacgccgcat ccggcacctg agcaccattg cctgctgtta
     2161 aaaatcggtg tggggcgcag gccccacttt ttggagaact tgtatgatgt accacattcc
     2221 cggcgtgtta tcgccacagg acgtcgcccg ttttcgcgaa cacctggaac aagccgaatg
     2281 ggtggatggg cgcgtcacca ccggcgcaca aggtgcgcaa gttaagaaca atcaacaagt
     2341 agacacccgc agcgccctgt acgccgccct gcaaaatgag gtgctgaacg cggttaacca
     2401 acatgctttg ttctttgccg cggccttgcc gcgtaccctt tccacgccgc tgtttaatcg
     2461 ctatcagaac aatgaaacct atggttttca tgtggatggc gcagtacgca gccatccaca
     2521 aaacggctgg atgcgcactg acctttctgc cacgctgttt ttaagcgatc cagaaagcta
     2581 cgacggcggc gaactggtcg ttaatgacac cttcgggcaa catcgggtaa aactcccggc
     2641 aggcgatctg gtgttgtatc cctccagcag cctgcattgc gtgacacccg taacccgcgg
     2701 cgtgcgagtg gcatcattta tgtggatcca gtcgatgatc cgtgatgata aaaagcgcgc
     2761 catgctgttt gaactggaca acaatattca gtcgctgaaa agccgctacg gcgaaagtga
     2821 agagatcctg tcactgctca atctttatca taatctgctg cgggaatggt cggagatctg
     2881 acgcttgaac tgcccgagtt cgtccctata ttagctacac ttaactgtac aagtattgat
     2941 atggggagga cgatatggca tccggttggg ctaatgatga cgccgtcaac gaacagatca
     3001 acagtacaat tgaagatgcg attgcccgcg ctcggggtga aattccgcgt ggcgaaagcc
     3061 tgtatgaatg tgaagagtgc ggtgccccta tcccgcaggc ccgtcgggaa gccattcctg
     3121 gcgtgcgctt atgcattcat tgccaacagg agaaagattt acaaaaacca gcttatacag
     3181 gatataatcg cagaggttcg aaagacagcc agttacgtta actgcaactg acgggatgtt
     3241 tagtgagtgt aatttgtatt ttgcgtggca ttccgtgcct ttaacgctgc cgtgagcacc
     3301 acgcggtaca
//
LOCUS       DAZFRG010000368         3265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFRG010000368 DAZFRG010000000
VERSION     DAZFRG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.370"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..441
                     /locus_tag="ACHKUC_004807"
     CDS             <1..441
                     /locus_tag="ACHKUC_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620891.1"
                     /translation="NAAVCTPAMETAVKQAQYNAMKIDAAQLEPIAEGFMANINSTLD
                     TIGCTDVWPTGSIGINLPSFDTIIRKAKEAAISKACSIAREKMNSVMGNISESVSFDV
                     PGMGNIAGGSISTGTGSTAGGSINVNGNSTTGSDVWNSISDVMK"
     gene            492..3161
                     /locus_tag="ACHKUC_004808"
     CDS             492..3161
                     /locus_tag="ACHKUC_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO6620892.1"
                     /translation="MKIKMEQRSLDIMRIGYIPAAILFMCLLITQNAYAADLFTVPDT
                     DKSKLWFLDVLFPENLAQSPLASTMTILNSAILLVGGILAAYTLIAGTMSTAHDGEML
                     GKKWSSMWLPVRTALGTAMILPAAGGFCAAQVMVLWLINQGVGLADTVWNTYAANPSD
                     GAVITTSASYQELDRIAKTAFINNVCMLKAGQLWKKSHESALFPGVMPVFEMTSEKGK
                     YLYRYNYGANNAGNEMNKNLLVSKSACGSITLTDPEAKASYDEQSQAQAQAAIASGGM
                     YMSYMPQEIKTNISAVVEAHNAAFIALNNNMMTLAEKYVADNNIDIQSDINSATATYV
                     SVIDTAVRTTFSTGNQWDDFKDNVQKDGWFMAGAWSMKLIRIQDAINGAAHNLPVAGQ
                     QSMDYGDIFNSLNAVMAKVSHDMARSTTASRYANGIDAQNRTEANTTGKGGQGSKTDD
                     ASKIITSLKSEADKSISGAMAGFLSSSVINGRKQGKLVAFSTDTTAANLQAINPLLAV
                     KGLGDTLSTAGWSLLGGSAVVGAGLGIWTSFTANWLSGAFGAIGVVMPLVIPLWVAGA
                     TLAVVIPMLPYVMWFGVCVGWMILCLEAMVAAPLWVITHLHPDGDGVVGRGGAGYGLV
                     LSLTMRPALMVTGLIAAYTMLPILGGVINETFSGAFGMMSANAGISIIESLALIAVYI
                     ALMFTVVKKSLSLIHVIPDEIMKWLGVHSGQSMSGYAQSASKGVESAVLAKTVLDQLS
                     HSSNALGNQIRNGQMNKDREQQRELQQQQQVQQGKAQASSRASDTGSAFRTHMSNAGP
                     LDQQDEYQSLESANSAYSAAEAADAVGDTAGAAGYMDVAQKAANRAVSFGEHNRPLLP
                     LNLQSKASPIESFKTPGKGDGGGSSGGSEEPSQ"
BASE COUNT          933 a          635 c          835 g          862 t
ORIGIN      
        1 aacgctgctg tttgcacacc cgctatggaa accgcagtaa aacaggctca atacaacgca
       61 atgaaaatcg atgcagctca actggaaccc atagccgaag ggtttatggc aaacataaat
      121 agtacattag atactattgg ttgcaccgat gtatggccta ctggaagcat cggcataaat
      181 ctgccaagtt tcgatactat aatcagaaaa gcaaaagaag cagcaataag taaagcctgt
      241 tcgattgcca gagaaaaaat gaattctgtt atggggaata tatcggaaag cgtttctttt
      301 gatgttccgg gtatggggaa tattgctggg ggtagcattt caacaggaac aggatcaact
      361 gcgggaggaa gcattaatgt taatggaaac tccacaacag gttctgatgt atggaacagt
      421 atcagtgatg tgatgaagta aaatataaaa atgtaactca cttggtgcgt tgcataaaaa
      481 agggagaaaa aatgaaaata aaaatggagc aacgctcttt ggatataatg aggataggat
      541 acatcccggc agccattctt tttatgtgtt tgctgattac gcaaaatgca tacgcagcag
      601 atttatttac ggttcctgat acggataaat caaaactgtg gttcttggat gttttatttc
      661 ctgagaattt agcccaaagc ccgttagcca gcacaatgac aatcctcaac agtgccattt
      721 tgttggttgg tgggatttta gcagcataca cgctaatagc cggaacgatg tcaacggcgc
      781 atgatggcga aatgctgggt aagaaatggt cctctatgtg gcttcctgtc aggacagcgt
      841 taggaaccgc aatgatactt cccgcagccg gtgggttctg tgcagctcag gttatggtgt
      901 tatggctgat aaatcagggt gtaggtcttg ccgatacggt ctggaatacg tatgccgcaa
      961 atccatcaga tggtgctgtt atcaccacgt cagcgtccta tcaggagctt gaccgcatag
     1021 caaagacggc attcatcaat aatgtttgta tgttgaaggc aggccagcta tggaaaaaat
     1081 cgcatgagag tgcgttgttt cccggtgtta tgcctgtgtt tgaaatgacg tcggaaaaag
     1141 gaaagtattt ataccgatac aattacggtg ctaataatgc tggtaatgaa atgaataaaa
     1201 acttactggt atccaaatca gcgtgtggaa gtatcacatt gacagaccca gaggctaaag
     1261 cgtcttacga tgaacagtct caagctcagg cacaggcggc tatcgcatca ggtggaatgt
     1321 atatgagcta catgccgcag gaaatcaaaa ccaatatatc agccgttgtt gaagctcata
     1381 atgcagcttt tattgccctg aataacaata tgatgacgct ggcagaaaaa tatgtcgctg
     1441 ataataacat tgatattcag tctgatataa acagtgcaac tgctacctac gtttctgtta
     1501 tcgatacggc ggtcagaact acattttcta caggcaacca gtgggatgac ttcaaggata
     1561 acgtccagaa ggatggctgg ttcatggcgg gggcatggag tatgaagttg atccgcattc
     1621 aggatgcaat aaatggtgct gctcacaatc ttcctgtcgc ggggcaacag agtatggatt
     1681 atggcgatat ttttaacagt ctgaatgctg ttatggcaaa agtgtctcac gacatggcga
     1741 gatcgacgac ggcatcaaga tacgcgaatg gtattgatgc gcaaaaccga acagaagcca
     1801 ataccactgg taagggggga caggggtcta aaacagatga tgccagtaaa ataatcactt
     1861 cactcaagag tgaagcagat aagagtattt ctggcgcaat ggctggtttc ctgagttcaa
     1921 gcgtaatcaa tggcagaaaa caaggaaaac tcgtcgcatt ttcaacagat acaacggcag
     1981 caaatttaca ggctatcaac cccctgttag ccgttaaagg ccttggcgat acactcagta
     2041 ccgccgggtg gagtttatta ggtggctctg ctgttgttgg tgcaggtctt ggaatatgga
     2101 cgtcatttac tgcaaactgg ttgtctggag cctttggtgc aattggtgtc gttatgccac
     2161 tggtgatccc gctatgggtt gctggtgcta cattagctgt tgttattcca atgctgccat
     2221 acgtcatgtg gtttggtgtc tgcgtgggct ggatgattct gtgccttgaa gcaatggtgg
     2281 cagcaccatt atgggtaatc acacacttac atccagacgg agatggtgtt gtaggacgag
     2341 gcggtgctgg ctatggtctg gtgctgtcgt taactatgcg cccggcactg atggtaacgg
     2401 gactaatagc cgcttatacg atgcttccga tacttggtgg ggtgattaat gaaacgttct
     2461 ccggcgcgtt cggtatgatg tcagccaacg ccgggattag catcatagaa tcactggccc
     2521 ttattgctgt ttacattgcg ctgatgttta cggttgtgaa gaaatcgctt tcgctgatcc
     2581 atgttattcc agatgaaatt atgaagtggc ttggcgttca tagtggtcag agcatgtcgg
     2641 gttatgctca gtcggcatca aaaggtgtgg aatcggcagt actggcaaaa acagtattag
     2701 atcagttatc acattcttcg aacgcgttag gtaatcagat cagaaacggg caaatgaata
     2761 aagaccgtga acaacaacgt gagcttcaac agcagcaaca agttcaacaa gggaaagctc
     2821 aggcttccag tagagccagt gatactggat ctgcattccg cacccatatg agtaatgcag
     2881 gaccgcttga tcagcaggat gaatatcaat cccttgaaag tgccaactct gcttacagtg
     2941 cggctgaagc tgcagatgct gttggtgata cagcgggagc tgctggctat atggatgtcg
     3001 ctcaaaaagc agctaacaga gcggtatctt ttggcgagca taacaggcca ttgttgccac
     3061 taaatcttca aagtaaagcg tctcctatag agagttttaa gactccgggt aaaggtgatg
     3121 ggggcggatc ttctggtgga agtgaagaac cgagtcaatg atgaataaag gtattagagg
     3181 gcttcggtcc tctttttaat ggattgtatc cttcatcctt attgtatttt cgcctttgac
     3241 gtgtttttag gataaataca tcatc
//
LOCUS       DAZFRG010000369         3264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFRG010000369 DAZFRG010000000
VERSION     DAZFRG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.371"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..347)
                     /gene="ydbL"
                     /locus_tag="ACHKUC_004809"
     CDS             complement(21..347)
                     /gene="ydbL"
                     /locus_tag="ACHKUC_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO6620893.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWMRKF"
     gene            complement(355..540)
                     /gene="ynbE"
                     /locus_tag="ACHKUC_004810"
     CDS             complement(355..540)
                     /gene="ynbE"
                     /locus_tag="ACHKUC_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO6620894.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(537..3176)
                     /gene="ydbH"
                     /locus_tag="ACHKUC_004811"
     CDS             complement(537..3176)
                     /gene="ydbH"
                     /locus_tag="ACHKUC_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO6620895.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLSVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLCDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
BASE COUNT          793 a          900 c          744 g          827 t
ORIGIN      
        1 ccgatattgc ggttttgcta ttaaaatttt cgcatccatt taccgtttat cccttgtaca
       61 tattccccgg gtttggcccg ttctacgagc ttttgcccgg cgagtttcgc gatatcatcc
      121 accgacacat tattctgctt tgccagttgt tgatagctcg ctttacgttc ggcattgatg
      181 tcggttacta atttctcagt ttcagcatct gttttcagcg caaccagata accataaaat
      241 gtttccccta cccgcccctg agttctggct tcatccagag tcaatgccat tacattgctg
      301 cttaccaagc caacaagaaa tgcacaaaga agtaatgttc ttttcatcat cacctcaaaa
      361 aagatcgcta cgggtctcaa gcagctcttc gacatctttg tctgccttga tgatgatctc
      421 atgctcaatt ttaacgttca tattgatagt gatcggttcc ttaggtgcag cgacttcaat
      481 gcgaggcgta cagccaacca gcataaaaga tgacgtcaac gcagccagta aaattttcat
      541 tgtttatcct cacactcttt tccttgcgga cagtcatttc ccggcagacg cgcgttctgc
      601 tccagccatg cctggagatt atcgccaaag cgtaaactgc gccacagcgt aaaaatattc
      661 tcttcatgat ggtaattaag atttaccgta ccgcttttac catcaacgcg actggtacct
      721 gtaatattgg cctgcatggt taataaaccg agattatcta aattaatttt tgtcgatgaa
      781 cggctaattt ccatatagcg caaccagtta attgctgaac ccgcggtcat attgtctttc
      841 accaccgcat ctgcggtgtc tttatccagc cgcaaggtca tcggcccgct atttgccagc
      901 cagccgtctt tcactatcca tttttcgttg tttagccata acggcaatgc accactaaat
      961 gccccggaca tggcgaattg tttcggattg acggcgctaa ccagttcgct ggaggaaaga
     1021 ttattcagtc gcaacagtgc cggatcatgt tgcggcatac gtaattgttt catcagcacg
     1081 ttaccgccta acacatcgac gctaacatca gtcaacagca atggttcttc ttcagtccac
     1141 ggataacgac cttgcaaatc agccgtaata tttttcgctg tcaccagatt aatcacttcg
     1201 gcaattcgca acgtaacggg accgcgagtc cccagatgcc aggctccatc ggcaaaacgg
     1261 aaaggcagga caaaatcgac accgttaacc tgattatctg gcatccaggc actaccgcct
     1321 ttcaacacgc cgtgccctcc cgcgcggaat ccttgttcag gcgcagctga aaatgcaacc
     1381 tgagcataca gttcaccatc gcataagttc atcttccagt cgggtggcac cagcggctgg
     1441 aatacagtca gtgattgttt aggccaccag gcgttaccgc gcagacgaat accgtcccag
     1501 cgaccattta cccgaaccgg gccaatctca ccagcatgta aatcgccttt aaagaggaaa
     1561 tacgtcggat cgcgcccatc gacgctaaat tttaaggttg atggcggtaa cacactgccg
     1621 ccagtgaaca gcgtttgccc ggcgtccagt gacagagcgc cgctgaagga gggatgctgt
     1681 gcgtcacgta cccagacgac gggcttgtcg agaattaatc tcggcttttc taccgtcatc
     1741 gtaccgtatt gtaactggtc gaaaccggtg gagagatcgg tcagcgtaat cgtgctgtca
     1801 tgccactcac cttttcctgc gacatcccag gtggcattca tcggtgtaaa actccctttt
     1861 ccccagtagc gccactgcca gcggccagcg tcagggagaa aatcattcgc cagcccatcc
     1921 atatgcagca cgaaatcgcc cagttcattt tcatgcgcct gcaaaatggc ctgcaaacgt
     1981 ccgtcaacac cacgttgggt gacttttaca cccgctaaag gccagcggat ttcatcgatg
     2041 tccagcgaat caatgactct tccctttgaa cgaagtaacg cgcctggctc aaaggtcagc
     2101 gttgggtcag acagacttcc acttagctgc gcaggtaaac gggcatataa aatgaggtcc
     2161 gcctgtttcg cttcaccggt cagctgcaga ggcagctggc tgttatccat gcttaatttt
     2221 cctgggccaa aatttaacac cgcgttgcct ttacccgctt gcccctgggt cagcacactc
     2281 agtcgcccgc tgaccagagc gttctcgagc cctgcctgcc agttgtcgac tttgacaccc
     2341 agtcggccac ttaaaggaaa acctgcatac ggccagctcc agcgaccatc gcttacggtc
     2401 aattgttgac gagtaatttg ccacggcaaa tcgagcaacg gatcgccgtt atcccgtgcc
     2461 agcacaatca attgcccgct attttcctgc cagtccagct cggcatccac cagtgaaggc
     2521 tcctgcggta agtttaacgt cgcagtagca tgaccactta ccggaagtcc atccggcacg
     2581 agcggcatag taaattcccc caccagtttt accggcggct gattttcaaa cgcggcgaca
     2641 tccagttcgc tgactgtaag ttgttgccct ttcagctggc cttgaaattt aactttttcg
     2701 ccctgataac gcagttgctg gatatcggag gttaatgcga gagagagttt tccctgccat
     2761 tcctgccagg gagaaaaaat cagtttatcg atattgatcc aggtgttagg cagcatggac
     2821 tgccactgcg cgagggtttt tggagcggct ggcgattgct ccgtctgcgg caatttcgcc
     2881 agacaagcag aatcaagttc taccgtgccg acgctcaata accagcggct gggatgtgaa
     2941 aggctggcgt tggtgatatg cgcaagctga caatctccca ccagataacg gagatcgggg
     3001 atgattaaac ctttacgcgt aatgcgtggg ctttcatcta atgcaatacg tgtcccgagc
     3061 ggtagccaga tgcccgccag cgtgggaacc cacagcccga gcgtcatcag cagcgtcaac
     3121 ggcacaagaa taatcagtaa taacagcgcg agaacggctt tatatttacc cagcatgggt
     3181 agttaatatc ctgatttagc gaaaaattaa gcattcaata cgggtattgt ggcatgttta
     3241 accgttcagt tgaaggttgc gcct
//
LOCUS       DAZFRG010000370         3226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFRG010000370 DAZFRG010000000
VERSION     DAZFRG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.372"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..635)
                     /gene="yeeO"
                     /locus_tag="ACHKUC_004812"
     CDS             complement(<1..635)
                     /gene="yeeO"
                     /locus_tag="ACHKUC_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6620896.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGIL"
     gene            796..871
                     /locus_tag="ACHKUC_004813"
     tRNA            796..871
                     /locus_tag="ACHKUC_004813"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:829..831,aa:Asn,seq:gtt)
     gene            complement(909..1859)
                     /gene="cbl"
                     /locus_tag="ACHKUC_004814"
     CDS             complement(909..1859)
                     /gene="cbl"
                     /locus_tag="ACHKUC_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO6620897.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1961..2878)
                     /gene="nac"
                     /locus_tag="ACHKUC_004815"
     CDS             complement(1961..2878)
                     /gene="nac"
                     /locus_tag="ACHKUC_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO6620898.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLREDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT          894 a          821 c          707 g          804 t
ORIGIN      
        1 atcaatatgc cgctaataat aatattaaga atattcaggc taccgttaat cagtagcggt
       61 attttcgtat tccctgcacc acgaagtgcc ccgctaccaa taagagtgat ggcagctgct
      121 ggataactga gtacggtcag ctccagataa gtcaacgcca gtgctttaac gtctgtcgtg
      181 gcatcacccg cgacgaaatc aataatttgt tcgccaaaat gatgaattag cgttgccaac
      241 agtacggcaa acaacgtcat gatcaccaat gactgccgcg tcgccaccct cgctcgtcgt
      301 cgatctcgct taccgagact aaatgctaca acgacagtag taccaagatc gatagctgca
      361 aaaaaagcca taatgaccat attgaagctg tccgccaacc ccacgccggc catcgcatct
      421 tttcccagcc agctgaccag aaaagtgctc aggaccccca tcaacaggac acaggcgttc
      481 tccatgaaga taggaacagc aagcggggtt atctcgcgcc agaacaacac tttgtagctc
      541 ttgcgtttag agtgccagcg agtgccgtga acaacctggc gtaaagcaga ggagatattc
      601 aaagccgacc ttaattgcag aaagtgaaac cacatttcaa atgatgaggg agaatcagca
      661 aagctgcaaa gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt tgatagaaac
      721 ggcaaacagt tgggaaattt aaaaatcggg tttgacaaaa tatttttcgc cgttaagatg
      781 tgcctcaaca gcgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
      841 gtcactggtt cgagtccagt cagaggagcc aaatttctga aaagcccgct tttatagcgg
      901 gctttttgct atatctgata atcaatttcc tcttcactgt tttccatcac ctggcgcttg
      961 atatcctcaa ctgacagtcc tgcattacaa agttccagaa agcgccagac atagttacgc
     1021 tgaagttgcc ctcgcttcag tcccaaccag acagtattag catcgaaaag atgccgcgta
     1081 tccaggcgga ttaaattctt ttcctcttgt tcgccgctgg attgttcggc aactaatccg
     1141 atcccaagcc caagagcaac ataggtttta atgacatcag aatcctgtgc acttaataca
     1201 atatctgccg gcaaaccttt gcgggcaaat gcgtcatcaa tgcgtgagcg ccccgtaatc
     1261 ccctgtcggt aagtaattaa cggccacttc gctattgatt ccagcgtcaa cggtgtaatt
     1321 tgcgtcaagg gatgatcgag tggaacaagc aaactatggt gccaacgaaa ccacgggaag
     1381 gcgacgagct gcgggtcatt actcaaacgc tcgctggcga taccaatatc agcttcgcca
     1441 ttttgcaaca acgtcgcaat ttcctgtggt gtaccctgga ttagctccag ccgaacctcc
     1501 gggaaaagtt cgcgaaaagc tttaatgacc tcaggcaagc tataacgtgc ctgagtatgc
     1561 gtcgttgcaa tagtgagaac gccagacgta tcgttggtaa acaggtctgc gagccgacga
     1621 acattactgg cttcattcag aatgcgttct gcaatgacca gtaatgcttt gcccggttca
     1681 gtcatgccca gcagtcgctt acctcgtcga acaaatattt cgatgccaag ttcatcctcc
     1741 agttcccgaa tatgacggct aacgcctgac tgtgaggtaa aaagcatatt cgcaacctct
     1801 gtcaggttgt aatcctgacg tgcagcctcg cggattatct ttagttgttg gaaattcacg
     1861 gtaaactccg ggcagttcag atatcccgtt attgttaaag tctaatgccc ggcataacaa
     1921 ataataaaaa cccgcatctt attccatccc gatataacgc ttagctcacc aattgccact
     1981 gccttttttc catcactgga gaactaatca ctgacattaa caactctttc actgcctgtg
     2041 cctgtggtga caagtttgct ctggcgggta aatttaagga caaagagaga ctcattgaag
     2101 gagtggtaat gcgtgacatc cacccattta ctgcgccaca taacgaacgc gcagccgatt
     2161 caggtaatac tgcaacgccc atgccgctgg caatcgctgc ggtaagcgtg gcaatagact
     2221 caatttcacc aatgactttt gccgtgagcc gccgtaggga aaaagcctca tcaacacgaa
     2281 gtctaacagc gctgtaatca ctggggagaa aaaggttcat ttgcgcaata gcattaacat
     2341 caacgctttg ccccgggcaa tcttgagttc ctaccaggaa aagatcttct ctcagcaaag
     2401 cctgactgga taccccagcc acaggggaat gctcataaat caccgccata tcgagttggt
     2461 gatttatcag tttttcgtta agcactgcac cactattttc atgaagatag ataacgacct
     2521 ccgggaattc agcgcgaacc gcctgtaata agggcatggt gatggatgac gcagcggttc
     2581 ctggcgcaaa gccaatcgag acttgccccg ataatgactg accaacgtta tgcaccgcca
     2641 gttgggcctg ctcacactga cgtaaaatgg cccgtgcatg ggtatagaga atttttccgg
     2701 cgcctgttgg cgtaacgccc cgttttgtgc gaatcaaaag ctgttgattt aactcacctt
     2761 ccagtgtggc aacctgctgg ctgagcgctg gttgtgcaat atgcaatact tcagcagcct
     2821 gggtcaggct accaatatct acaattttta cgaagtattt caggcgtctg aagttcatgt
     2881 tgcctccggt ttttaagaat cggcccaagt gccgccatta cttacaacca gaatgcaaga
     2941 tgcttgccag ttctatttta gcgttgatgc gcaagctaac caactgtcaa ataagagatt
     3001 atgatagatt cgtcattggc cctttttatc agcagtcgca ttcacctgcc ctataaaagg
     3061 ggggtatgca ccacgatggt tcattaccca ataagattga aagctcacca ctttgttgaa
     3121 attgtcagca aacaaacaaa aaaatgcatt tcaccctttg acatcaccat gcactgccat
     3181 taatatgcgc cccgttcaca cgattcctct gtagttcagt cggtag
//
LOCUS       DAZFRG010000371         3219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFRG010000371 DAZFRG010000000
VERSION     DAZFRG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.373"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..506
                     /gene="hlyC"
                     /locus_tag="ACHKUC_004816"
     CDS             <1..506
                     /gene="hlyC"
                     /locus_tag="ACHKUC_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hemolysin-activating
                     lysine-acyltransferase HlyC"
                     /protein_id="HFO6620899.1"
                     /translation="NNPLEVLGHVSWLWASSPLHRNWPVSLFAINVLPAIQANQYALL
                     TRENYPVAYCSWANLSLENEIKYLNDVTSLVAEDWTSGDRKWFIDWIAPFGDSGALYK
                     YMRKKFPDELFRAIRVDPKTHVGKVSEFHGGKIDKQLANKIFKQYHHELITEVKNKSD
                     FNFSLTG"
     gene            518..>3219
                     /gene="hlyA"
                     /locus_tag="ACHKUC_004817"
     CDS             518..>3219
                     /gene="hlyA"
                     /locus_tag="ACHKUC_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin hemolysin HlyA"
                     /protein_id="HFO6620900.1"
                     /translation="MPTITTAQIKSTLQSAKQSAANKLHSAGQSTKDALKKAAEQTRN
                     AGNRLILLIPKDYKGQGSSLNDLVRTADELGIEVQYDEKNGTAITKQVFGTAEKLIGL
                     TERGVAIFAPQLDKLLQKYQKAGNKLGGSAENIGDNLGKAGSVLSTFQNFLGTALSSM
                     KIDELIKKQKSGNNVSSSELAKASIELINQLVDTAASINNNVNSFSQQLNKLGSVLSN
                     TKHLNGVGNKLQNLPNLDNIGAGLDTVSGILSAISASFILSNADADTGTKAAAGVELT
                     TKVLGNVGKGISQYIIAQRAAQGLSTSAAAAGLIASVVTLAISPLSFLSIADKFKRAN
                     KIEEYSQRFKKLGYDGDSLLAAFHKETGAIDASLTTISTVLASVSSGISAAATTSLVG
                     APVSALVGAVTGIISGILEASKQAMFEHVASKMADVIAEWEKKHGKNYFENGYDARHA
                     AFLEDNFKILSQYNKEYSVERSVLITQQHWDTLIGELAGVTRNGDKTLSGKSYIDYYE
                     EGKRLEKKPDEFQKQVFDPLKGNIDLSDSKSSTLLKFVTPLLTPGEEIRERRQSGKYE
                     YITELLVKGVDKWTVKGVQDKGAVYDYSNLIQHASVGNNQYREIRIESHLGDGDDKVF
                     LSAGSANIYAGKGHDVVYYDKTDTGYLTIDGTKATEAGNYTVTRVLGGDVKILQEVVK
                     EQEVSVGKRTEKTQYRSYEFTHINGKNLTETDNLYSVEELIGTTRADKFFGSKFTDIF
                     HGADGDDHIEGNDGNDRLYGDKGNDTLRGGNGDDQLYGGDGNDKLIGGTGNNYLNGGD
                     GDDELQVQGNSLAKNVLSGGKGNDKLYGSEGADLLDGGEGNDLLKGGYGNDIYRYLSG
                     YGHHIIDDDGGKDDKLSLADIDFRDVAFKREGNDLIMYKAEGNVL"
BASE COUNT         1049 a          551 c          750 g          869 t
ORIGIN      
        1 gaaacaatcc attagaggtt cttgggcatg tatcctggct ctgggccagt tccccactac
       61 acagaaactg gccagtctct ttgtttgcaa taaatgtatt acctgcaata caggctaacc
      121 aatatgcttt attaacccgg gaaaattacc ctgttgcata ttgtagttgg gctaatttaa
      181 gtttagaaaa tgaaattaaa tatcttaatg atgttacctc attagtcgca gaagactgga
      241 cttcaggtga tcgtaaatgg ttcattgact ggattgctcc tttcggagat agcggtgccc
      301 tgtacaaata tatgcgaaaa aaattccctg atgaactatt cagagccatc agggtggatc
      361 ccaaaactca tgttggtaaa gtatcagaat ttcatggagg taaaattgat aaacagttag
      421 cgaataaaat ttttaaacaa tatcaccacg agttaataac tgaagtaaaa aacaagtcag
      481 atttcaattt ttcattaaca ggttaagaag taattaaatg ccaacaataa ccactgcaca
      541 aattaaaagc acactgcagt ctgcaaagca atccgctgca aataaattgc actcagcagg
      601 acaaagcacg aaagatgcat taaaaaaagc agcagagcaa acccgcaatg cgggaaacag
      661 actcatttta cttatcccta aagattataa aggacaaggt tcaagcctta atgaccttgt
      721 caggacggca gatgaactgg gaattgaagt ccagtatgat gaaaagaatg gcacggcgat
      781 tactaaacag gtattcggca cagcagagaa actcattggc ctcaccgaac ggggagtggc
      841 tatctttgca ccacaattag acaaattact gcaaaagtat caaaaagcgg gtaataaatt
      901 aggcggcagt gctgaaaata taggtgataa cttaggaaag gcaggcagtg tactgtcaac
      961 gtttcaaaat tttctgggta ctgcactttc ctcaatgaaa atagacgaat tgataaagaa
     1021 acaaaaatct ggtaacaatg tcagttcttc tgaactggca aaagcgagta ttgagctaat
     1081 caaccaactc gtggacacag ctgccagcat taataataat gttaactcat tttctcaaca
     1141 actcaataag ctgggaagtg tattatccaa tacaaagcac ctgaacggtg ttggtaataa
     1201 gttacagaat ttacctaacc ttgataatat cggtgcaggg ttagatactg tatcgggtat
     1261 tttatctgcg atttcagcaa gtttcatttt gagcaatgca gatgcagata ccggaactaa
     1321 agctgcagca ggtgttgaat taacaacgaa agtactgggt aatgttggaa aaggtatttc
     1381 tcaatatatt attgcacagc gtgcagcaca ggggttatct acatctgctg ctgctgccgg
     1441 tttaattgct tctgttgtga cactggcaat tagtcccctc tcattcctgt ctattgccga
     1501 taagtttaaa cgtgccaata aaatagagga gtattcacaa cgattcaaaa aacttggata
     1561 cgatggtgac agtttacttg ctgcttttca caaagaaaca ggagctattg atgcatcgtt
     1621 aacaacgata agcactgttc tggcttcagt atcttcaggt attagtgctg ctgcaacgac
     1681 atctctggtt ggtgcaccgg taagcgcgct ggtaggggct gttacgggaa taatttcagg
     1741 catccttgag gcttcaaaac aggcaatgtt tgaacatgtc gccagtaaaa tggccgatgt
     1801 tattgctgaa tgggagaaaa aacacggcaa aaattacttt gaaaatggat atgatgcccg
     1861 ccatgctgca tttttagaag ataactttaa aatattatct cagtataata aagagtattc
     1921 tgttgaaaga tcagtcctca ttacccagca acattgggat acgctgatag gtgagttagc
     1981 tggtgtcacc agaaatggag acaaaacact cagtggtaaa agttatattg actattatga
     2041 agaaggaaaa cgtctggaga aaaaaccgga tgaattccag aagcaagtct ttgacccatt
     2101 gaaaggaaat attgaccttt ctgacagcaa atcttctacg ttattgaaat ttgttacgcc
     2161 attgttaact cccggtgagg aaattcgtga aaggaggcag tccggaaaat atgaatatat
     2221 taccgagtta ttagtcaagg gtgttgataa atggacggtg aagggggttc aggacaaggg
     2281 ggctgtatat gattattcta acctgattca gcatgcatca gtcggtaata accagtatcg
     2341 ggaaattcgt attgagtcac acctgggaga cggggatgat aaggtctttt tatctgccgg
     2401 ctcagccaat atctacgcag gtaaaggaca tgatgttgtt tattatgata aaacagacac
     2461 cggttatctg accattgatg gcacaaaagc aaccgaagcg ggtaattaca cggtaacacg
     2521 tgtacttggt ggtgatgtta agattttaca ggaagttgtg aaggagcagg aggtttcagt
     2581 tggaaaaaga actgaaaaaa cgcaatatcg gagttatgaa ttcactcata tcaatggtaa
     2641 aaatttaaca gagactgata acttatattc cgtggaagaa cttattggga ccacgcgtgc
     2701 cgacaagttt tttggcagta aatttactga tatcttccat ggcgcggatg gtgatgacca
     2761 tatagaagga aatgatggga atgaccgctt atatggtgat aaaggtaatg atacgttgag
     2821 gggcggaaac ggggatgacc agctctatgg cggtgatggc aatgataagt taattggggg
     2881 gacaggtaat aattacctta acggcggtga cggagatgat gagcttcagg ttcaggggaa
     2941 ttctcttgct aaaaatgtat tatccggtgg aaaaggtaat gacaagttgt acggcagtga
     3001 gggagcagat ctgcttgatg gcggagaagg gaatgatctt ctgaaaggtg gatatggtaa
     3061 tgatatttat cgttatcttt caggatatgg ccatcatatt attgacgatg atggggggaa
     3121 agacgataaa ctcagtttgg ctgatattga tttccgggac gttgccttta agcgagaagg
     3181 gaatgacctc attatgtata aagctgaagg taatgttct
//
LOCUS       DAZFRG010000372         3137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFRG010000372 DAZFRG010000000
VERSION     DAZFRG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.374"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            153..>3137
                     /locus_tag="ACHKUC_004818"
     CDS             153..>3137
                     /locus_tag="ACHKUC_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6620901.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGAITSELPFGYYSITPAEVNAKGEVVTPQTYNGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSVI
                     DTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATDHLTVDSYSTVNLTEST
                     GWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVVSGVFD
                     INSSDYVLNADLVNDRTWDTAQANYGYGVVAMNSDGHLTINGNGDVDNGTELDNSSVD
                     NVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNSNATFSAANKADLGAYTYQAE
                     QRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVS
                     YFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVD
                     QDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKA
                     GYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTY
                     SEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTD
                     ANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT          868 a          705 c          727 g          837 t
ORIGIN      
        1 gctttatata tgtccgcaat cggacttagg ggaaagaata attgaaccat tcctctataa
       61 cgccgcataa tcgtaaacgc gaaacataat atgttttcta tgcattgata attgatggat
      121 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
      181 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcaaact
      241 atgatacctg ggggtatcat gacaacacca cgaccgattt agtttggccg tatgacactg
      301 acaatgatgg aattgccgat gctggcggca tggacccgat gggttcagac ggtaactacg
      361 taacttacaa tggttttgtt tactataaca atgccaatgg cgatttcgat accgtcttta
      421 aaggcgatac cgttaacggt accatttcta cttattactt gaatcatgat tacgctagcg
      481 gttctgttaa ccaggtagat atcagcaatt cagtaattca tggggcaatc acctctgaac
      541 tgccatttgg ttattacagc attactcctg cagaagttaa tgctaaaggt gaagttgtaa
      601 ctccacagac ctataatggc tataatttct ataacggaat cactactgac cttgactaca
      661 attggtacga tggtgacgta ttcactctga acgtatccaa ttccaccatt gatgatgact
      721 atgaagctct ttacttcact gacacatatc ttgatggtga taaatctaag tcaaccaatg
      781 aaacgttcta taccgctctg ggtgttgcag taaatcttga tgttgaaagc aatatcaata
      841 ttactaacaa ctcccgtgta gctggtattg cgctgtctga aggcaataca agcaacacga
      901 cttatactag cgaatatcat cagtgggaca acaacattaa tgtgtctaac tccactgtaa
      961 cttcagggtc acagactccg ctggaagaaa gcaatggctt cttcggtaaa tctgctgaac
     1021 caagtgatta cgctggtaat ggtggcgaaa atgacgtagc tctgtcattc tctgacaatg
     1081 ctggctcaaa ttattccatg aaaaacaatg taaactttga ccattcaacc ctgttgggtg
     1141 atgtcgagtt cacaagccac tggaataacg atggcgtatt cttctattca accggccatg
     1201 acagcaatgg tgacggtgtc cttgatacca acggtggttg ggtagatgat gctcagaacg
     1261 ttgatgaact gaacattact ctgaacaacg gtagtaagtg ggttggctca gcaaacatga
     1321 gtgctgaagt aattgctccg gcagatatgt atgatgttgc gccgaacagc ctgacccctg
     1381 gtgccactat cgaagctaac gattggggtc gtattattga caacaaggta ttccagagcg
     1441 gcgtatttaa cgtagcactg aacaatggtt cagaatggaa taccgttaat agttcagtaa
     1501 ttgatacatt agctgtaaat aatggctctc aggtaaatgt tactgattcc tctctggtct
     1561 cagataccat tggtttaacc aacggctcct cactgaatat tggtgaagat ggtttggttg
     1621 ctacagacca cctgactgtt gatagctaca gtaccgttaa tttaaccgaa tctactggct
     1681 ggaataacta ttctaacctg tatgccaata ccatcaccgt aactaacggt ggtgtactgg
     1741 atgtgaacgt tgatcagttt gatactgaag ctttccgtac tgacaaactg gaactgacta
     1801 gcggcaacat cgctgaccac aacggcaatg tagtttctgg tgtgttcgat atcaacagca
     1861 gcgattatgt tctgaacgct gacctggtga acgaccgtac ttgggatacc gctcaggcta
     1921 actacggcta cggtgttgtt gctatgaact ctgatggtca tctgactatc aacggtaacg
     1981 gcgacgtaga caacggtact gaactggata acagttctgt agacaacgtt gttgcggcaa
     2041 ccggtaacta caaagttcgt atcgacaacg caactggcgc tggcgcaatc gctgattaca
     2101 aagataaaga aattatctat gtaaacgacg tcaacagcaa cgcgaccttc tctgctgcta
     2161 acaaagctga cctgggtgca tacacctatc aggctgagca gcgtggtaac accgttgttc
     2221 tgcaacagat ggagctgacc gactacgcta acatggcgct gagcatcccg tctgcgaaca
     2281 ccaatatctg gaacctggaa caagacaccg ttggtactcg tctgaccaac tctcgtcatg
     2341 gtctggctga taacggcggc gcatgggtaa gctacttcgg tggtaacttc aacggcgaca
     2401 acggcaccat caactatgat caggatgtta acggcatcat ggtcggtgtt gataccaaaa
     2461 ttgacggtaa caacgctaag tggatcgtcg gtgcggctgc aggcttcgct aaaggtgata
     2521 tgaatgaccg ttctggtcag gtggatcaag acagccagac tgcctacatc tactcttctg
     2581 ctcacttcgc gaacaacgtc tttgttgacg gtagcttgag ctactctcac ttcaacaacg
     2641 acctgtctgc aaccatgagc aacggtactt acgttgacgg cagcaccaac tccgacgctt
     2701 ggggctttgg tttgaaagcc ggttacgact tcaaactggg tgatgcaggt tacgtgactc
     2761 cttacggcag cgtttctggt ctgttccagt ctggtgatga ttaccagctg agcaacgaca
     2821 tgaaagttga tggtcagtct tacgacagca tgcgttatga actgggtgta gatgcaggtt
     2881 ataccttcac ctacagcgaa gaccaggctc tgactccgta cttcaaactg gcttacgtct
     2941 acgacgactc taacaacgat aacgatgtga acggtgattc catcgataac ggtactgaag
     3001 ggtctgcggt acgtgttggt ctgggtactc agttcagctt caccaagaac ttcagcgcct
     3061 ataccgatgc taactacctc ggtggtggtg acgtagatca agactggtcc gcgaatgtgg
     3121 gtgttaaata tacctgg
//
LOCUS       DAZFRG010000373         3131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFRG010000373 DAZFRG010000000
VERSION     DAZFRG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.375"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(26..553)
                     /gene="mobB"
                     /locus_tag="ACHKUC_004819"
     CDS             complement(26..553)
                     /gene="mobB"
                     /locus_tag="ACHKUC_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO6620902.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRSGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(535..1119)
                     /gene="mobA"
                     /locus_tag="ACHKUC_004820"
     CDS             complement(535..1119)
                     /gene="mobA"
                     /locus_tag="ACHKUC_004820"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO6620903.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQESGEWFLFCPCDT
                     PYIPHDLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1189..1458
                     /locus_tag="ACHKUC_004821"
     CDS             1189..1458
                     /locus_tag="ACHKUC_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO6620904.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1535..2521
                     /gene="srkA"
                     /locus_tag="ACHKUC_004822"
     CDS             1535..2521
                     /gene="srkA"
                     /locus_tag="ACHKUC_004822"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO6620905.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTVTFIEQAKVLQEPPLQLTPMY"
     gene            2538..>3131
                     /gene="dsbA"
                     /locus_tag="ACHKUC_004823"
     CDS             2538..>3131
                     /gene="dsbA"
                     /locus_tag="ACHKUC_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO6620906.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKQVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYA"
BASE COUNT          789 a          758 c          762 g          822 t
ORIGIN      
        1 cgcgtttgct ccagcctact cttgcttatc cgtcctgctt ttgcatccac tccaccacaa
       61 aatcagccag cccctcaaca tcattaatat ccagtaacgc gacatcaaga ttaagcggca
      121 catcactggc tacagcaata acatgcctgt ctatcactaa ttcttccggt cgatgtccgg
      181 ctccatcgcg aaacagcaca atctttgcga tctcttcatg cttaaaccct tcgaccagaa
      241 tcaaatccag ctttgaggta tccattcgac ttgcgagaaa atgtagatcc agctcttctt
      301 cgtctggtgt ttctgtcatc aaggcccatc gctgttggct ggcaacgatg gtttgcgccg
      361 cgccagcctt gcgcagctca tagctatctt tgcccggttt atcaacatcc atatcatggt
      421 gcgtatgctt aatcagcccc gaacggatcc ctctggcgca taatgccggg atcaattttt
      481 tcagcagcgt tgtttttccg gtgccactcc atgcagcaaa ggcgagtaac ggtatcatcg
      541 tttttcctgc catcgggcaa gctcctctgg cgtattcacg tttataaatg cctctttacg
      601 atcgctgaaa tcaaccgcat gaccgccagc cagacgcata aatgccataa cccggcgttc
      661 tcctgcttgc agatattcca gtaataaagg ctcaattgcg cggtttacca gagcaatagt
      721 cgggtgatcg cgttcaccgt cgtggaccca cacgacaggc gcatctttgc gctgatgatt
      781 aagccgggag gctaaatcat ggggaatgta aggcgtatcg cacgggcaaa acaaaaacca
      841 ctcacccgat tcctgctgca ttactgaaag cattcctgcc agagggccag ggtaatccgc
      901 cagtgaatct tcaatcactt tcagaccgct tacctgatag atttcctgat gacgattagc
      961 attaaccacg acgtgagaaa gctgcgtcat aagcgcgtca gcgacatgtt gccataatgg
     1021 tttgccgttt aattcaagca atcctttatc tacgccgccc attcgtctgg ctttaccgcc
     1081 cgccagcaca acgcctgtta tcgtcgtcat cagattcacc gatatcgcct cttttattgt
     1141 gggattgacc ctgctaacgt gtctgtctca agagtaagga gcattactat gaaatgtaaa
     1201 cgtctgaatg aagttattga actcctccag ccagcctggc aaaaagagcc agaccttaac
     1261 ctgctgcaat ttttgcagaa actggcgaaa gagtcaggtt ttgacggcga actggcggat
     1321 ttgacggatg acattctgat ctatcacctg aaaatgcgtg attccgcgaa agatgcggtg
     1381 atcccgggtt tgcagaaaga ttatgaagaa gatttcaaaa cggcactatt acgcgcacgt
     1441 ggcgtaatta aagagtaaaa gcttgtaagc ggcgcaacca aaatcatcgt gaaatgatat
     1501 ccttcatcat tcgtaatgtt ttccggatga tgggatgaac aacagcgctt ttactttcca
     1561 gacactacac ccggatacca tcatggacgc tctgtttgag catgggattc gggtggattc
     1621 cggtcttacc ccgcttaaca gctatgaaaa ccgtgtctat caatttcagg acgaagatcg
     1681 tcgacgtttt gtcgtcaaat tttatcgccc tgaacgttgg acagccgatc aaatcctcga
     1741 agaacatcaa tttgcgttgc agctggttaa cgatgaagtt ccggtcgcag cacctgtggc
     1801 ctttaatggt cagactttat tgaaccatca gggattttat ttcgctgttt ttccaagcgt
     1861 cggtggtcgc cagttcgaag ctgataatat cgatcagatg gaagcggttg ggcgttattt
     1921 agggcgtatg caccagacgg ggcgcaaaca gctttttatc catcgcccga ccatcggttt
     1981 gaatgaatat ctcattgagc cacgcaagct gtttgaggac gctacactga taccttccgg
     2041 gttgaaagcg gcattcctga aagcgacaga tgagctgatt gccgccgtta cagcacactg
     2101 gcgggaagat ttcaccgttc tgcggctaca tggagactgc cacgccggga atattctctg
     2161 gcgcgatggt ccaatgtttg ttgatctgga tgatgcacgt aatggtccag ccgttcagga
     2221 tttgtggatg ttgctcaatg gtgataaagc cgagcagcgg atgcaactgg aaactattat
     2281 tgaggcttat gaagaattta gcgagttcga caccgctgaa atcggactga ttgaaccttt
     2341 acgcgccatg cgtttggttt attatcttgc ctggctaatg cggcgttggg ctgatcccgc
     2401 gttcccgaaa aatttcccgt ggttaaccgg ggaagattac tggctacgac agacggtgac
     2461 ttttatagaa caggcaaaag ttctacaaga accccctttg caattaacac ctatgtatta
     2521 atcggagaga gtagatcatg aaaaagattt ggctggcgct ggctggttta gttttagcgt
     2581 ttagcgcatc ggcggcgcag tatgaagatg gtaaacagta cactaccctg gaaaaacaag
     2641 ttgctggcgc gccgcaagtg ctggagtttt tctctttctt ttgcccgcac tgctatcagt
     2701 ttgaagaagt tctgcatatt tctgataacg tgaagaaaaa actgccggaa ggcgtgaaga
     2761 tgactaaata ccacgtcaac ttcatggggg gtgacctggg caaagagctg actcaggcat
     2821 gggctgtggc gatggcgctg ggcgtggaag acaaagtcac agttccgctg tttgaaggcg
     2881 tacaaaaaac ccagaccatt cgttcagcat ctgatatccg cgatgtattt atcaacgcag
     2941 gtattaaagg tgaagagtac gacgcggcgt ggaacagctt cgtggtgaaa tctctggtcg
     3001 ctcagcagga aaaagctgca gctgacgtgc aattgcgtgg tgttccggcg atgtttgtta
     3061 acggtaaata tcagctgaat ccgcagggta tggataccag caatatggat gtttttgttc
     3121 agcagtatgc t
//
LOCUS       DAZFRG010000374         3093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFRG010000374 DAZFRG010000000
VERSION     DAZFRG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.376"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..756)
                     /gene="ulaD"
                     /locus_tag="ACHKUC_004824"
     CDS             complement(106..756)
                     /gene="ulaD"
                     /locus_tag="ACHKUC_004824"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6620907.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(770..1234)
                     /gene="ulaC"
                     /locus_tag="ACHKUC_004825"
     CDS             complement(770..1234)
                     /gene="ulaC"
                     /locus_tag="ACHKUC_004825"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO6620908.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(1244..1549)
                     /gene="ulaB"
                     /locus_tag="ACHKUC_004826"
     CDS             complement(1244..1549)
                     /gene="ulaB"
                     /locus_tag="ACHKUC_004826"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO6620909.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(1565..2962)
                     /gene="ulaA"
                     /locus_tag="ACHKUC_004827"
     CDS             complement(1565..2962)
                     /gene="ulaA"
                     /locus_tag="ACHKUC_004827"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6620910.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMTTIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLEIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
BASE COUNT          763 a          858 c          770 g          702 t
ORIGIN      
        1 tatatactta ttattcaaca atatagcttg attaagttca tccccggcgg ggagcgcttt
       61 ttcatagatg ccaagcggga tttgtttgga caacatatcc gctccttagc cccacagttc
      121 agcgatggaa cgtttgaact gacgtgcggc ttccaccgga gaagcggcat cacggatact
      181 acggcccgcg ataaagacgt gaatcggaat acccttgaac agcggcagat cttccagcgc
      241 caggcctccg gtgacggtga ctttgaagcc catatcggaa agacgtttga tcgcggtgat
      301 gtccgcttcg ccccacgcca cgcctgcggc ctgcgcgtca cggctgcggt gataaaccac
      361 ctgctgaatg cctgcatcgc gccactgttg cgcctgttcc caggtccagt aaccggtcag
      421 ttcgatctgc acgtcgccgt taaactcttt tgccacgtcc agcgcgcctt tggcggtgtt
      481 gatatccgca cagcaaatta ccgtcaccca gtcagcgttg gcttcgaagc acatacgcga
      541 aaggattttg cctgcatcgg caattttggc gtctgccagt acgattttgt gcgggtagag
      601 cgctttcagg tcacgaaccg cacgcacgcc ttcgcccacg cacagaatgg tgcccacttc
      661 gataatgtcg acttcttcgg caatcaggcg agtggtttcg taggcgctat ccatagtctg
      721 gttgtccagc gcgacttgca acatcggtaa tgacatgttc aattccttct taagctgccg
      781 cgttggtgcg gtcaatgaga tccagtactt cctgctcggt acggcaggcg cgtaaacggt
      841 caaaattctc ttcatcttca aacaggttga cgatctgcat gatgcccact tcctggtgag
      901 tattggcatc gaccgccgcc atggtgatga ggatatccac cggatcgttg tcgtcgtggt
      961 taaattccag cggctttttc agcgttacca gagagaaacc tgttttctta acaccctctt
     1021 ccggccgccc gtgcggcatc gccagccccg gagcgataac gaaataaggg ccaaactgtt
     1081 ccacgccatc cagaatcgcc tggtaatagc gcggctcgac aacatccgcc gccaccagca
     1141 ggtcaacgcc gattttcact gcgtcctgcc atgtctctgc ttcagcctgc aggcggatgg
     1201 atttattttc cgccagcgaa tcacgtaatt tcatggcgcg tccttacttc acatcctgcg
     1261 ggaaatgctc tttgatcact tccagcagtt tcgggccaaa gtcagcagga gagagcatgt
     1321 tgcgcacgcc aaccacgtat ttgttgccgg tcacggtgat ttcgcccgca atgtgcgtag
     1381 aagcgatgat gatatccgcg ccgctcaact cgcttttgta ttcgccaacc gcgcagctgt
     1441 ttaccgtgtg gtcaatgttt gattgggtta aaaactggtc cactttcatc ttcatgatca
     1501 tggaactgcc ttgtccgttg ccacacacag ccagaatacg tacggtcata atcaaaactc
     1561 cttattaagc agactgttct gccagttgtt tttctgcatc ttcttctgcg cgcagcgcgc
     1621 ggccagcgaa gaacatataa gccagtgcaa ttacaatgat gacggccata aaggcgatac
     1681 cgatggagaa gaagccttgc atcatcggcg gtgccagaat cgaccagtcc gccatgccca
     1741 tccaggcact cataccggtg agtttcaccg cccagacgca accaaagatt tcgatcatcc
     1801 ccatcaccag acaaatcttc agcgccgcac gccagccgcc gaagtggtta gcgaacacgc
     1861 cgatggtggc gttggagaag aacatcggga taaagccggg aataatcagg atcgaggaac
     1921 cgcaggcgac caggatgcca accgcaatca gctgaccgat ggtgccccac ataaagcccc
     1981 aaaccacggc gttcggcgcg aagctataga tagccgcaca gtcaatctcc agaaccgcac
     2041 cagggatcag gcgctgggag ataccgttaa atgcttcaga gagttccgcc acaaacatgc
     2101 gcacgccctg agtgatgatg aagatcgcca ccgcgaagga gaaaccagtt tgcaggatgt
     2161 acaccgtcca gttcactttg cctgccatcg cctgcacagt gtcgataccg aaggagagca
     2221 gaatggcacc aaagaagatg gtcatcacaa tcgccgtgga gacgatgttg tcgtggaaaa
     2281 tattcagcca gcctggcagt ttgaggtctt caacgctttc ctcttttttg ccgaggaacg
     2341 gcgcaacttt ataggcaatc catgatgcaa actgttgctg gtgaccgatg gagaaaccgc
     2401 agccgtccgt cacttcctga gtcggcttgt acatcatgtt ggaggtaatg ccccagtaga
     2461 gcgaaaccag aatcgccgtg cagataatgg ttgtccacat ggagtagccg aaaataaaca
     2521 acgtaacggc aatcagcccc gcctgctgga acatgatatg accagtcagc atgatggtac
     2581 gaataccggt aatgcgacgc agcagcacgt aacagatgtt cagcgccagc gctaacaaca
     2641 ccgcgtaacc tacccagcta taggcatcgc ccatgcggtc gatggttgtc atcatcgacg
     2701 cgtaagtatc ggaaattgcg ccgttaatgc cgtagacttc ggacattttc gccaccaccg
     2761 gtttgaaggt gctggtgagg atgccggacc ctgcctgcaa caacatgaaa ccaattatgg
     2821 ttttaatcgt gcctttaata ataacgctga cacttttgcg cagtaggatg tagcccagac
     2881 aggtcacaat acccagcaac aacggggcat tggtcatgac ctggttaaaa aacacggtaa
     2941 agatgttgta gaggatctcc ataacgatct cctgaagaag aggtaaccgg gtattccgca
     3001 cacccggaat gttgtgcact cactctaatt ttcaaaagta atcacaacaa gattatttat
     3061 gattaaatgt gacgcgaccc gcaaataatt cca
//
LOCUS       DAZFRG010000375         3065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFRG010000375 DAZFRG010000000
VERSION     DAZFRG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.377"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..1711)
                     /locus_tag="ACHKUC_004828"
     CDS             complement(116..1711)
                     /locus_tag="ACHKUC_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFO6620911.1"
                     /translation="MQKILRIDSNDNLIVALKDLHAGESFSWDDDNITLVTDVKAKHK
                     FATQDIPLDGIVSMYGTPVGKATRPIVKGEAITVDNIRHYAAPVTLEDVEPYHWQAPD
                     VSEWSTRTFKGYVRDDGRVGTASYWLVFPLVFCENRNVSKLTNALNDALGYTNNSLKK
                     FALNLTSGSDELIETARMFPHIEGVRCITVTSGCGGATSDCETMCDVLAAYADHPNVI
                     GMTVFSLGCEKAQQKMFKDALARRNPEFDKPALYFLQQEWDSEERMMQTALQQTFEAM
                     KAVKPTERVEVPLSCLKVGMKCGGSDGFSGISGNPAMGLVSDWLTTLGGASGLAEFPE
                     LCGAEGDMVKRCINLEDKKKFLNLMQGYEKTANFFDTTIADNPSFGNIADGLITDAIK
                     STGAAKKGGRAPIVSVCDYAEPMPDSGLSLVCTPGNDVDAVTGLVAAGCNVVIFSTGL
                     GTPTGNPIVPVLKISTNSTIARRLSDMIDFDCGPVIDGTPLEDVSRELFEKVIETASK
                     DYVVKADKLEQFDFMLWKRSLDL"
     gene            complement(1745..3046)
                     /locus_tag="ACHKUC_004829"
     CDS             complement(1745..3046)
                     /locus_tag="ACHKUC_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620912.1"
                     /translation="MANITQYDNPLLNSAIQKSWKRLVPLMFIIYFVAFIDRVNVGFA
                     KDAMKLDIGLSESAFALGAGIFFAAYALFGIPANLILNKIGAQKWLSITTAIWGLLSV
                     MTGFVTSETQFIILRFLLGLGEAGFYPGILLLASIYFPNKVRGSVVGIFVLGVPLALT
                     LGSPISGALLELHGWMGRPGWFWMFVIEGLPALVLGIFAWFYLDDNPTKARFLTQEEK
                     DALNKQLESERQKTETSSVISALKNAKVWHLALIYGTIQISVYGLMFFLPSQVASLMG
                     ESLGFKQSLVAAIPWACSAFGVYYIPRIADRNPSRRVAISVMCMLAAAVGLALSAFAG
                     PVVAIGALCLSAVGFLSVQPIFWTFPPQLLTGPALAAGIGFCTTMGAFCSFLAPLIRV
                     EVDRAMDSSIAGLMALSVITIGCALLIGLLQKSQQAAKAYS"
BASE COUNT          896 a          788 c          622 g          759 t
ORIGIN      
        1 tagacaaaaa aaaaccatcc aaatctggat ggctattaag aagaaccaaa aatttaacgc
       61 atgcatacga taaaaaatgc gccagtctac atacactgac gctaatgatt ggtcactaca
      121 agtctaacga ccgcttccac aacataaaat caaactgttc gagtttgtct gccttcacga
      181 cgtagtcctt actggcagtt tcaataactt tttcaaataa ttctctactc acatcttcca
      241 gcggagtgcc gtcaatcact gggccacagt cgaaatcaat catgtcagac aatcgtctgg
      301 caatcgtgct gttggtggat atttttaaca ccggaacaat cgggttaccg gtgggcgtac
      361 caagacccgt cgagaagata accacattac atcctgctgc taccaatccg gttaccgcgt
      421 ccacgtcatt gcccggagta cacaccaacg ataatccact atcgggcata ggttcagcgt
      481 aatcgcatac actcacaatt ggcgctcgcc cgcctttttt cgctgctccg gtagatttta
      541 ttgcgtcagt aatcaaccca tccgcgatgt tgccaaaact tgggttatcc gcaatggtgg
      601 tatcgaaaaa gttcgctgtc ttttcgtagc cttgcattag attgaggaac ttttttttat
      661 cctcaagatt aatacaacgc ttaaccatat caccttctgc cccacataat tctggaaatt
      721 ctgctaaccc ggaagcgcca ccaagcgttg tcaaccagtc agaaaccagc cccattgcag
      781 ggttgccgga gataccagaa aaaccatcgg aaccaccaca cttcatcccc actttgagac
      841 aggaaagagg gacctctact ctttctgtcg gcttcaccgc tttcattgcc tcgaaagtct
      901 gctgtaaagc ggtctgcatc atccgctctt cgctgtccca ctcctgttgg agaaagtaga
      961 gcgcaggttt atcaaattca ggattacgtc tcgccagcgc atccttaaac attttctgct
     1021 gcgctttttc acatcctaaa ctgaataccg tcataccgat tacattcgga tggtcagcat
     1081 aagcagctaa tacatcgcac atagtttcac agtctgaagt tgcaccacca caaccactgg
     1141 tgaccgtaat acagcgaaca ccttcaatat gcggaaacat tctcgcggtt tcgatcagtt
     1201 catcgctacc ggacgtcaga ttaagagcaa actttttcag tgagttattg gtataaccca
     1261 aagcatcatt caaagcattg gttaacttac tgacattacg gttctcgcaa aataccaatg
     1321 gaaagactaa ccagtaactg gcagtaccca ctctgccatc gtcacggaca taacctttaa
     1381 aagtgcgggt gctccattca gagacatccg gtgcctgcca gtggtagggt tcaacatcct
     1441 ccagtgtgac aggagctgca tagtggcgaa tattatcaac ggtaatcgct tctcccttaa
     1501 cgatgggacg cgtggccttg ccaactggcg taccgtacat cgaaacaatg ccatccagag
     1561 gaatatcttg cgtcgcaaac ttatgtttag ccttaacatc agttactaac gttatgttgt
     1621 cgtcatccca cgagaacgat tctccagcgt gcaggtcttt caacgcgaca atgagattat
     1681 cgttactgtc gatacgtaat attttttgca taatcatatt cttccagtca gatgttcaat
     1741 cttattaact gtatgctttt gctgcctgtt ggctcttttg cagcaatcca atcaataatg
     1801 cgcagccgat agtaattaca gacaacgcca tcaatccggc gatgctgcta tccattgccc
     1861 gatcaacctc aacacggata agaggagcaa ggaacgagca gaaagcgccc atcgtggtgc
     1921 aaaaaccgat ccccgcagcc agtgccggac cagtaagaag ttgtggaggg aaagtccaga
     1981 atattggctg tactgagagg aaaccaaccg cacttaaaca aagtgcacca atcgccacaa
     2041 caggccccgc aaatgcggaa agggcaaggc cgacagcagc agccaacata cacataaccg
     2101 aaatggccac acgccgggaa ggattgcggt cagcaattcg cggaatgtaa tagacaccaa
     2161 aagcagagca cgcccatggt attgccgcga ctaatgactg cttaaagcca aggctttctc
     2221 ccatcagcga tgcaacctgt gacggaagga aaaacattaa accatatact gatatctgga
     2281 tggtgccgta gattaatgcc agatgccaaa ctttggcatt tttcaatgct gaaatcactg
     2341 aactggtttc tgttttctgt cgttcagatt ccagttgctt attcaaagcg tctttttctt
     2401 cctgggtaag aaatcgcgct ttcgtcggat tatcatcaag gtagaaccag gcaaagatac
     2461 caagcactaa tgccggcaat ccctcaatga caaacatcca gaaccatcct ggacgtccca
     2521 tccagccatg tagttcaagt aaagctcccg atattggcga accgagcgtt aacgccagcg
     2581 gcacccccaa cacaaatatt cccacgaccg acccgcgaac cttgttagga aaataaatgg
     2641 atgcgagcag taaaattccg ggatagaacc ccgcctcgcc cagccctaac agaaagcgca
     2701 aaataataaa ttgtgtttca ctggtgacaa atcccgtcat taccgagaga aggccccaaa
     2761 ttgcagttgt aatacttaac cacttttgtg cgccgatttt attgaggatc aggttagctg
     2821 gaatgccgaa aagtgcatag gcagcgaaaa atattcctgc accgagagcg aatgccgact
     2881 ctgataatcc aatatccagt ttcattgcat ctttggcaaa gccaacattt acccgatcaa
     2941 taaaggcaac aaaatatatt atgaacatta agggtactaa acgtttccac gatttttgaa
     3001 ttgcggaatt caacagcgga ttatcgtatt gggttatatt agccataaat aaatccttag
     3061 ttctg
//
LOCUS       DAZFRG010000376         3055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFRG010000376 DAZFRG010000000
VERSION     DAZFRG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.378"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1208)
                     /locus_tag="ACHKUC_004830"
     CDS             complement(<1..1208)
                     /locus_tag="ACHKUC_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6620913.1"
                     /translation="MTDGGGMHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLAD
                     ARVRRDETRKLLANGVDPGDKKKNDKVEQSKARTFKEVAIEWHGTNKKWSEDHAHRVL
                     KSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSG
                     LIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFI
                     RSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGSKMRTTHLVPLSTQALAILK
                     QIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRTMACSSLVE
                     SGLWSRDAVERQMSHMARNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFE
                     YAKINNPLKQ"
     gene            complement(1451..1535)
                     /locus_tag="ACHKUC_004831"
     tRNA            complement(1451..1535)
                     /locus_tag="ACHKUC_004831"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1499..1501),aa:Leu,seq:caa)
     gene            1731..2750
                     /gene="ahr"
                     /locus_tag="ACHKUC_004832"
     CDS             1731..2750
                     /gene="ahr"
                     /locus_tag="ACHKUC_004832"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO6620914.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(2754..>3055)
                     /gene="idnK"
                     /locus_tag="ACHKUC_004833"
     CDS             complement(2754..>3055)
                     /gene="idnK"
                     /locus_tag="ACHKUC_004833"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate kinase"
                     /protein_id="HFO6620915.1"
                     /translation="YRDILRKGSPHVHFLWLDGDYETILARMQRRAGHFMPVALLKSQ
                     FEALERPQADEQDIVRIDINHDIANVTEQCRQAVLAIRQNRICAKEGSASDQRCE"
BASE COUNT          745 a          765 c          742 g          803 t
ORIGIN      
        1 tactgtttta atggattatt aatctttgca tattcaaatg gactgataaa cctttctctg
       61 tttacatcca gaaaatcggc ccaccactgt agcatcaatc gccgttcttc cagatgctct
      121 gctttatgga tatacgcggc cctcactgaa tttcgcgcca tgtggctcat ctgacgttca
      181 acagcatcac gagaccacag acctgattcg accaatgaac tacaggccat tgttcgaaag
      241 ccatgaccac aaacctctac ttttgtatca taccccatga cccgtaacgc actatttacc
      301 gtattctcac tcatgggttt gtgcgaatcg tgatcaccaa taaatatcaa gtcatgggcc
      361 ccataaaact gttttatctg ctttaaaatt gcaagagctt gcgttgaaag aggcactaga
      421 tgcgttgtac gcatttttga gcctctatgg gaatgtttca ctccaggaat aggctcccgc
      481 tccggtggga tagtccatat agacgcttcg aaatcgatct ctgaccaacg agcaaaacgc
      541 agctcactgg accgaataaa gatcagcaaa gtgagttcta tcgcccatcg ggttagcggc
      601 ctaccagtat agctatctat ttttgtaagc aactcaggga tgcgctttaa ttcaagcgcg
      661 ggacgatgtt gtcgattaca ggaagcaacc gccccagcca tctcttgtgc cgggttataa
      721 tcaattaacc cactttgcac tgcatagcgc atgatggctg tagtgcgctg ctgaagacga
      781 gcggccactt caagacgtcc agacatttct acggccttaa taggtgctaa taaatctcga
      841 gtttttaact cagcgatatt acgttcacca agcgctgcaa aaagattatc ttcaagactt
      901 tttagcacac gatgggcgtg atcttcagac cactttttat tggtgccatg ccactcaatc
      961 gcgacttctt taaaggttcg tgctttactc tgttcaacct tatcattttt ctttttgtct
     1021 cccggatcga cgccattcgc aagcagctta cgcgtctcgt cacgacgtac tctggcatcc
     1081 gctagtgtga tttcaggata aaccccaagt gccagcattt tttgctttcc ctcataacgg
     1141 tactgcaaac gccagtactt agaaccattt ggatggacaa gcagatgcat accgccacca
     1201 tcagtcagct aatattgctt atccgttggc ttggctgctc tgactttgat atctgttaat
     1261 gccatcgtat tccctccctg ttggtacaag cattatcgaa ccggaaatac catcatctgt
     1321 accaacatat gttggtagat gtacgttgat atcggttgac cttgagagag ttaatatagc
     1381 gggaaagata gataaacact ggatttcagg cataaaaaaa gacctcagtt gaggtctatt
     1441 tacatacttt tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt
     1501 gaatcaactg cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt
     1561 gtggattata cctgtcgcac gccaccatgc aagcgccagc ttgctcgcac ctcgctaagt
     1621 gctgaaattt tcagcattgc atacagcgat gtgtaacctt tgtcacactc caggcacccc
     1681 gccctgccat gctctacact tcccaaacaa caccagagaa ggaccaaaaa atgtcgatga
     1741 taaaaagcta tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag tacgatcccg
     1801 gtgagctgag gccacaagat gttgaagtgc aggtggatta ctgcgggatc tgccattccg
     1861 atctgtcgat gatcgataac gaatggggat tttcacaata tccgctggtt gccgggcatg
     1921 aggtgattgg gcgcgtggtg gcactcggga gcgccgcgca ggataaaggt ttgcaggtcg
     1981 gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac gcctgtatta
     2041 gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattatgaat cgcggtggct
     2101 ttgccgagaa gttgcgtgcg gactggcaat gggtgattcc actgccagaa aatattgata
     2161 tcgagtccgc cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca ctgttgatgc
     2221 accatatcac tgctaccagc cgcgttgggg taattggtat tggcgggctg gggcatatcg
     2281 ctataaaact tctgcacgca atgggatgcg aggtgacagc ctttagttca aatccggcga
     2341 aagagcagga agtactggcg atgggtgccg ataaagtggt gaatagccgc gatccgcagg
     2401 cactgaaaac tctggcgggg cagtttgatc tcattatcaa tactgtgaac gtcagcctcg
     2461 actggcagcc ttattttgag gcgctgacgt atggcggtaa tttccacacc gtcggtgcgg
     2521 ttctcacgcc gttgccggtt ccggccttta cgctgattgc gggcgatcgc agtgtctctg
     2581 gctctgctac cggtacacct tatgagttgc gtaagctgat gcgctttgcc gcccgcagca
     2641 aggttgcgcc gactaccgaa ctgttcccga tgtcgaaaat taacgacgcc atccagcatg
     2701 tgcgcgacgg taaggcgcgt taccgcgtgg tgttgaaagc cgatttttga aaatcattcg
     2761 cagcgctgat ctgaggcgct gccctctttc gcacatattc tgttttgtcg tatcgccagc
     2821 acagcctgcc gacattgttc ggtgacattg gcaatatcat ggttgatatc aatgcgcaca
     2881 atatcctgtt catctgcttg tggacgctcc agcgcctcaa actgactttt tagtaacgct
     2941 accggcataa aatgcccagc ccgacgctgc attcgcgcga gaatagtttc ataatcgcca
     3001 tctaaccaga ggaaatgaac atgggggcta cccttgcgta aaatatcacg atact
//
LOCUS       DAZFRG010000377         3037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFRG010000377 DAZFRG010000000
VERSION     DAZFRG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.379"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..2134
                     /locus_tag="ACHKUC_004834"
     CDS             137..2134
                     /locus_tag="ACHKUC_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO6620916.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2197..2535
                     /locus_tag="ACHKUC_004835"
                     /pseudo
     CDS             2197..2535
                     /locus_tag="ACHKUC_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
     gene            complement(2545..3037)
                     /locus_tag="ACHKUC_004836"
                     /pseudo
     CDS             complement(2545..3037)
                     /locus_tag="ACHKUC_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          674 a          751 c          767 g          845 t
ORIGIN      
        1 gtgtccgcta ttgccagtac acctcaacat tccaccatgc attccgatta acgccgcata
       61 gccagttgaa ctttgctact ttgtgagagg tagtaccttc tatccagtgc gaatttaatt
      121 aatggaataa atgattatga gtgaaaatga tacaatccca aagaagtcta caagtcagat
      181 taacaaagcg gtattcttta catctgcttt gctaattttc cttcttgtcg cctttgccgc
      241 cgtattcccg gatgtcgccg acaaaaattt taaactactt cagcaacaaa tcttcacgaa
      301 tgccagctgg ttctacatcc ttgctgtggc cctgatttta ctgagtgtca cgttccttgg
      361 actctcacgc tacggtgata tcaagctggg cccggaccat gcgcagcctg atttcagcta
      421 ccactcctgg tttgcgatgc ttttttcggc agggatgggg atcggcctga tgttctttgg
      481 cgttgccgaa cctgtaatgc attatctttc gccacccgtt ggcactccag aaaccgttgc
      541 ggcagccaag gaagcaatgc gtctgacctt tttccactgg ggactgcacg catgggcaat
      601 ttatgccatt gtggcgctga ttctggcgtt cttcagttac cgtcacggtc tgcctttaac
      661 tctgcgctcc gcactctatc ccattattgg cgatcgcata tacggacctg taggacatgc
      721 ggttgatatt ttcgctgtta taggcacggt ctttggcgtt gcgacatcac tgggttacgg
      781 tgttttgcag gtgaatgccg gtttgaacca tcttttcggg gtgcccatca atgaaacggt
      841 gcaggtaatt ctgatcgtgg tcatcacggg gttagcgacg atttcagtgg tgtccggtct
      901 ggataaggga atacgtatcc tgtctgaact caatctgggt ctggctttgt tgctcctggc
      961 gctggtcctg tgtctgggac caaccgtgct tctgctgaag tcatttgtgg aaaatacggg
     1021 cggttatctt tcggaactgg tgagtaaaac gttcaacctt tacgcgtatg agcccaagtc
     1081 gagcaactgg ctggggggct ggacattact gtactgggga tggtggcttt catggtcgcc
     1141 gtttgtgggg atgttcatcg cacgggtctc ccgcgggcga accattcgcg agtttgtcac
     1201 cggcgtgctg tttgttcccg cgggttttac gctaatgtgg atgacggtgt ttggtaacag
     1261 cgcgatctat ctcattatga accagggggc cacagacctc gccaatactg ttcagcagga
     1321 tgtgtcgctg gccctgttta atttcctgga gcatttcccg ttctcttctg tgctgtcatt
     1381 cattgcaatg gcgatggtca tcgtcttctt tgtaacgtct gctgattcgg gggcaatggt
     1441 tgtggatact ctggcatcag gtggagtggc aaacacaccc gtctggcagc gaatattctg
     1501 ggcctcgctc atgggcattg ttgcaattgc gcttctcctt gccggagggc taagtgcgct
     1561 gcaaacggtg acaatagcga gtgcattgcc cttctcagtg atcttactaa tatccatata
     1621 cggactttta aaagctttgc gccgggattt gaccaagcgt gaaagcctga gcatggcgac
     1681 aattgctcct acggctgcac gtaacccaat tccttggcag agaaggttac gcaatatcgc
     1741 gtatctgccg aagcgatctc ttgtgaaacg ttttatggac gacgttatcc agcccgccat
     1801 gacgctggtt caggaggaac tgaacaagca ggggacgata agccacatta gtgatgcagt
     1861 cgacgatcgt attcgtcttg aagtcgattt gggcaacgag ctgaatttta tatatgaagt
     1921 gaggcttcgc gggtatatct caccgacctt cgcgctcgcc gcaatggata atgatgagca
     1981 gcagactgaa caacatcgat attatcgcgc tgaggtttat ctcaaagaag gcggtcaaaa
     2041 ttatgatgtg atgggctgga accaggaaca gctgattaat gacatactgg accagtacga
     2101 aaaacacctg cacttcctgc acctggttcg ttaatagcaa catgccgtcc tgggggcggc
     2161 aattattatc tcggccgcaa tatgagggaa tgcagaatga tttcacgctg gaaatggatg
     2221 ctgaagcaga caattaaaaa actatggttc agggcaacgt tattcgcaat tgtcgcgata
     2281 ataacggccc ttttatcaat tctttttaaa tcaatgatac ctgagtcggt ttccgtgaag
     2341 gttggtgcgg aagcagtcga taacattctg aacatactgg catcgagtat gctggcagtg
     2401 accacatttt cgctgagtat catggtcaca gcctacggtt cagccactac taatgtgact
     2461 cccagagcta cgcgtttagt tgttgaagac gtcaccacac aaaatgtact ggccaccttc
     2521 atcggttctt ttctcttgaa gtggtcaaca aaaactggcc accgcgttag agtttttcca
     2581 gtatcgattt tccgattcgt ttggggtaac ccaccgttat attcgtgcgg tcttagtgcg
     2641 ctgtaatatc caacgatata gtccgttatg gcgtgagctg cctcgctgaa gcttacgtaa
     2701 cccaccaccg gcatccattc gttcttcaga ctcctgaaga agcgttccat tgggctgtta
     2761 tccctgcagt ttccgcgccg gctcatactc tgtctgatct ggtatcgcca caataactgc
     2821 cggaactgcc tgctcgtata atgactgccc tgatcgctgt ggaacatcac cccgccgggc
     2881 ttaccacggg tttcccatgc catttccagc gctttcatgg tgagcctgct gtccggcgag
     2941 aacgacatgg cccagcccac tggttttctt gcgaacaggt cgagaacaac ggcgaggtac
     3001 gcccagcgct tacccgtcca gatataggtc acatcac
//
LOCUS       DAZFRG010000378         3019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFRG010000378 DAZFRG010000000
VERSION     DAZFRG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.380"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..202
                     /locus_tag="ACHKUC_004837"
     CDS             <1..202
                     /locus_tag="ACHKUC_004837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620917.1"
                     /translation="PSFYIIIQFQLTPDERQCFSTGIKSQLLLSKTHSTNNISADIIL
                     TRTPHITEFSSDSITAQIKRTG"
     gene            669..1541
                     /locus_tag="ACHKUC_004838"
     CDS             669..1541
                     /locus_tag="ACHKUC_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6620918.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1913..>3019
                     /locus_tag="ACHKUC_004839"
     CDS             1913..>3019
                     /locus_tag="ACHKUC_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6620919.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLD"
BASE COUNT          780 a          775 c          800 g          664 t
ORIGIN      
        1 gccttcattt tacatcatca tccagtttca gttaaccccg gacgagcgac agtgtttcag
       61 tacaggaatt aaaagccagc ttttgctcag taaaacacac tcaactaata atatctcagc
      121 agatattatc ctgaccagga cacctcacat tactgaattc tcttccgaca gtatcactgc
      181 acaaataaaa agaacgggat aaatattcag accggaagaa tggtgtcaca aaagcattta
      241 atcattacgc atgaccagga agggaaacac ttaaccacat ggtatcaaca gatatcatta
      301 cgtgtgcact cttataaaaa acacacagaa gaagtaagat aattataaac gtaaatttaa
      361 tatctcccac tgacatctcc cccacaatac actaaaaaat actcacagca gtaaacacag
      421 atacacctga taatcataca gccgataaat attttcccct ttacggagaa atcagcctgc
      481 tgtttaacaa taacggcgta ttttcatgga aggagatcac ccagtaacgg ataagtcctg
      541 atctccttca tgttaatacg caacaatccc atcgattcaa aacaatttca gacagatatt
      601 atctccgtga atcccccgcc acctttccgg tgcgcggggt tttgtctttt ttcaccggga
      661 atacatgtat gaatccgtct gatgccattg aggcaattga aaaaccgctc tcctccctgc
      721 cttactcact ttcccgtcac atcctggaac atctgcgcaa actcaccagt cacgaacccg
      781 tgattggcat tatgggtaaa agcggggccg gtaaatcctc actctgtaat gcactgtttc
      841 agggggaggt caccccggtc agtgatgttc acgccggcac ccgggaagta cggcgcttcc
      901 gtctgagtgg ccacggtcac agcatggtta tcactgacct gcccggggtg ggcgagagcc
      961 gggacaggga tgcagagtat gaagccctgt accgtgacat tctgcccgaa ctggacctgg
     1021 tgctgtggct gattaaagcc gatgaccgcg ccctgtctgt ggatgagtat ttctggcgac
     1081 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
     1141 agccctgcca tgaatgggat atggccggca ttcagccttc tcccgcacag gcacagaaca
     1201 ttcgggagaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgttgccg
     1261 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctaatg acagcacttc
     1321 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
     1381 tccgcgctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
     1441 agagcgtctg tgttgcctct gttgcccgta cggttctgcg tgccgtccgt gacacggtgg
     1501 tctctgttgc ccgcgctgta tggaactgga ttttcttctg aacagtccgc cagggaaatg
     1561 cctgataaca ttacgttaac caactgaaaa catgacacaa acccctcata ctcctcctgt
     1621 ttctcttgcg tgaccgcctt gtcgttaata gaataaaacg atcgataaaa caggtatcga
     1681 tcgttactat cgatcgttta tagtggttaa tggtctgcga aaaggcagct gaatctcttc
     1741 atcatgcaga acggaattgc acacaacgga ctgatacttc tctggcggat gagagagggg
     1801 aagcatttat gtccgggaaa aaccacttta tctgagcatg ctgttttgta cctgccggta
     1861 tccacatttg tgggtaccgg cttttttatt caccctctgt aaggaaaagc tgatgaaacg
     1921 acatctgaac accagctaca ggctggtatg gaatcacatt acgggcaccc tggtggtggc
     1981 ctccgaactg gcccgctcac ggggaaaacg cgctggtgtg gcggttgcgc tgtctcttgc
     2041 tgctgtcaca tcagtcccgg cactggctgc tgacaaggtt gtacaggcgg gagaaaccgt
     2101 gaacgacgga acactgacaa atcatgacaa ccagattgtc ctcggtacgg ccaacggaat
     2161 gaccatcagt accggtctgg agtatgggcc ggataacgag gccaataccg gcggacaatg
     2221 gatacaaaat ggcggtatcg ccaacaacac tactgtcacc ggtggtggtc ttcagagagt
     2281 gaatgccgga ggaagcgttt cagacacggt tatcagtgcc ggaggcggac agagccttca
     2341 ggggcaggca gtgaacacca ctctgaacgg cggtgagcag tgggtacatg aaggcgggat
     2401 tgcaacgggt accgtcatta atgagaaggg ctggcaggcc atcaaatccg gcgcagtggc
     2461 aaccgacacg gttgtgaata ccggcgcgga agggggaccg gatgcggaaa atggtgatac
     2521 cgggcagacc gtctacggag atgccgtacg caccaccatc aataaaaatg gtcgtcagat
     2581 tgtggctgct gaaggaacgg caaataccac tgtggtttat gccggcggcg accagactgt
     2641 acatggtcac gcactggata ccacgctgaa tggggggtac cagtatgtgc acaacggcgg
     2701 tacggcgtct ggcactgttg taaacagtga cggctggcag attgtcaaaa acgggggtgt
     2761 ggccgggaat accaccgtta atcagaaggg cagactgcag gtggacgccg gtggtacagc
     2821 cacgaatgtc accctgaagc agggcggcgc actggttacc agtacggctg caaccgttac
     2881 cggcataaac cgcctgggag cattctctgt tgtggagggt aaagctgata atgtcgtact
     2941 ggaaaatggc ggacgcctgg atgtgctgac cggacacaca gccactaata cccgcgtgga
     3001 tgatggcgga acgctggat
//
LOCUS       DAZFRG010000379         3003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFRG010000379 DAZFRG010000000
VERSION     DAZFRG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.381"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..210
                     /gene="clpS"
                     /locus_tag="ACHKUC_004840"
     CDS             <1..210
                     /gene="clpS"
                     /locus_tag="ACHKUC_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO6620920.1"
                     /translation="TPMEFVIDVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVA
                     ETKVAMVNKYARENEHPLLCTLEKA"
     gene            241..2517
                     /gene="clpA"
                     /locus_tag="ACHKUC_004841"
     CDS             241..2517
                     /gene="clpA"
                     /locus_tag="ACHKUC_004841"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO6620921.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTLYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(2920..3001)
                     /locus_tag="ACHKUC_004842"
     tRNA            complement(2920..3001)
                     /locus_tag="ACHKUC_004842"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2971..2973),aa:Ser,seq:gga)
BASE COUNT          751 a          690 c          859 g          703 t
ORIGIN      
        1 actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga tgtagaacgt
       61 gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg agtctttacc
      121 gctgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga gaatgagcat
      181 ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg ggaggtgcct
      241 atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc gcgcgagcac
      301 cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa cccatctgcc
      361 cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga actggaagcc
      421 tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga cacacaaccg
      481 acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc ctccggtcgc
      541 aatgaggtta ccggtgcaaa cgttctggtc gctatcttta gcgaacagga gtcgcaggcg
      601 gcatatctgt tgcgcaaaca tgaagtcagc cgtctcgatg tggtgaactt tatctctcat
      661 ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc aaacagcgaa
      721 gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa tcagcttgcg
      781 cgagtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg tgctattcag
      841 gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggagtctgg tgtcggtaaa
      901 accgcgattg cggaggggct tgcatggcga attgttcagg gcgatgtgcc ggaagtgatg
      961 gctgactgta cgctttactc tctcgatatc ggttccctgt tagcgggcac aaaatatcgc
     1021 ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga cactaacagc
     1081 atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc tggtggccag
     1141 gtcgatgcgg ctaacctgat taaaccgttg ctctccagcg gtaaaatccg cgtaattggt
     1201 tcgacaacct atcaggagtt cagcaacatt tttgagaaag accgtgctct ggcgcgtcgc
     1261 ttccagaaaa ttgatattac tgaaccgtca atcgaagaaa ctgttcaaat catcaatggc
     1321 ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc ggtgcgtgcg
     1381 gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa agcgattgat
     1441 gttatcgacg aagcgggcgc tcgcgcacgt ctgatgccgg taagcaaacg caagaaaacc
     1501 gttaatgtgg cggatattga atccgtggtg gcccgtattg cacgcattcc agagaagagt
     1561 gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa aatgctggtc
     1621 ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc gcgtgcaggt
     1681 ttaggtcacg aacataaacc ggtcggttcg ttcctgtttg ccggccctac tggggtcggg
     1741 aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagcttct gcgctttgat
     1801 atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc tccgggatac
     1861 gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc acatgcggtg
     1921 ctgctgctgg acgaaatcga gaaagcgcac ccggacgtgt tcaacatcct cttgcaggtg
     1981 atggacaacg gtacactgac cgataacaac ggacgcaaag cggacttccg taacgtggtg
     2041 ctggtgatga ccactaacgc gggggtacgt gaaaccgagc gcaaatccat tggtcttatc
     2101 caccaggata acagcaccga tgcgatggaa gagatcaaga agatctttac accggaattc
     2161 cgtaaccgtc tcgacaacat tatctggttc gatcatctgt caaccgacgt gatccatcag
     2221 gtggtggata aattcatcgt cgagttgcag gttcagttgg atcagaaagg tgtttctctg
     2281 gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg ggcaatgggc
     2341 gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc caacgaactg
     2401 ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa agagaaaaat
     2461 gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc gcattaatct
     2521 gattgtctgg taggttggtg aagtccgtaa tctcgtcagg ggttacggac tttttattta
     2581 tggggggagg aggttcagac ccttttttta atgatgatgc taagttattg ataattagtg
     2641 ctgcgggtag gtaaggataa aaaagggtgg cagcaggaga ttgagatggt tttgctttat
     2701 taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc ttagaaagca
     2761 cacgtcgagc tggtggggat taatgctggg ttcactgccg tacaggcagc ttagaaattg
     2821 ccgcggatcc tgtctgccaa taatgacaag ttcactgccg tacaggcaga taaaatgcga
     2881 aaaaaaaccc gtactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg
     2941 aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca
     3001 cct
//
LOCUS       DAZFRG010000380         2979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFRG010000380 DAZFRG010000000
VERSION     DAZFRG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.382"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..560)
                     /locus_tag="ACHKUC_004843"
     CDS             complement(<1..560)
                     /locus_tag="ACHKUC_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6620922.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGYSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFR"
     gene            1895..>2979
                     /locus_tag="ACHKUC_004844"
     CDS             1895..>2979
                     /locus_tag="ACHKUC_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6620923.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFS"
BASE COUNT          785 a          587 c          724 g          883 t
ORIGIN      
        1 cggaataccg cctccgtttt ttcgcgaatg ttctgtgcct gtgcgggaga gggctgaatg
       61 ccggccatat cccattcatg gcagggctcc gttttgtcgg cctgtgtcac cacaaacagc
      121 acctgctgat gtccgcactg caggatgtgt cgccagaaat actcatccac agacagggcc
      181 cggtcatcgg ctttaatcag ccacagcacc aggtccagtt caggcagaat gtcacggtac
      241 agggcttcat actctgcatc cctgtcccgg ctctcgccca ccccgggcag gtcagtgata
      301 accatgctgt aaccgtgacc actcagacgg aagcgctgca cttcccgggt gccggcgtga
      361 acatcactga ccggtgtgac ctccccctga aacagggcat tacagagtga agatttaccg
      421 gccccgcttt tacccataat gccaatcacg ggctcgtgac gggtgagttt gcgcagatgt
      481 tccaggatgt gacgggaaag cgagtaaggc agggaggaga gcggtttttc aattgcctca
      541 atggcatcag acggattcat acatgtattc ccggtgaaaa aagacaaaac cccgcacacc
      601 ggaaaggtgg cgggggatcc acggagataa tatctgtctg aaattgtttg gaatcgatgg
      661 ggatgttgcg tattaacatg aaggagggca ggagttatcc gttattgggt gatatccttc
      721 catgaaaata cgcggttatt gttaaacagc aggctgatta ctccgtaaag gagaaaaata
      781 ttcatcttgc agcctgatta tcaggtgtgt ttatgtttac tgaggtgagt atttttcagt
      841 atattgtcgg ggcagtcagt gggagatatt aaatttacgt ttagagttat cttgcttctg
      901 ctgtgtattt ttataagagt gcgcacgtaa tgatatctgt tgataccatg ttgttaagtg
      961 tttcccttcc ggttcatgcg tacttgcaaa atggcatctg aatcgatcac ggtttggcca
     1021 aaccgtgatc gaatagagag gaatacgcac gtaatgatta aatgcttttg ttacaccatt
     1081 cttccggcat gaatatttat ccagttccct tttttatttg tgtagtgata ctgtcggaat
     1141 agcgttcagt aatgtgaagt gtcctgttaa aaatctttaa taaatgtgtc atcctggtca
     1201 ggaaatatct gctgagatat tattagttgg tggtgttttt actgagtaat agctggcttt
     1261 taattcctga actgaaatac tgtcgctcgt cagggtttaa ttaaaactgg atgatgatgt
     1321 gaaaatgaag gctggttttt gtgtttgtcg aagaagtgtt attttaatcc attctcatca
     1381 ttggctggcg attatacaga agtatttgaa gaaaaaatgg tgcattttat ctgtgtcagc
     1441 agggaagaag tattttattg atgtatcagt attaaatggt ttttgttatt tataaaaatg
     1501 tcatcctgtc cataaaatat tgtactcagc attcagtgca ggaactgtag tcttctttat
     1561 tttattcttc ttcaggaggg ggggtgattc ctgaaggcgt tattcccggt gttctgttta
     1621 tgattcttcc cggcaggaaa ccggtatctg caatacccgg gatgatgctt tttgttcgta
     1681 cgtggttgtg taaaatttga atagtggatg tgctccgaag ggaaggaggg gacacacaga
     1741 ttcattttgt ctttcttcag cagcaaaata cactgtggtt attatttaac cattaaatca
     1801 acaaaaaatt ttacctcata ttgatttgac tgctattttt gctattaata gccagaattg
     1861 cagtgctttt atatctgtgt atagtgaggt tactatgtca ttcggtcagc cttgtgatga
     1921 gtttcccctg agttcgctgc ctccactcat tcgtgatgcc gttattgaag ctcagcaaat
     1981 tacgcaggct ccccttgggc ttgtggcagc gtctgccctg ggcgctgttt cacttgtctg
     2041 ccagaatctg attgatgtct gcagactgaa tacactgcgg gggccggtct ctcttttttt
     2101 cctgacgctt gccgagtccg gagaacgaaa aaccgcagtg gacaaattat taatgaagcc
     2161 cctgtaccag caggagatgc agttatattc cagatacaaa agtgaactgg ctgtctggaa
     2221 aaacaaggaa gagcttttaa aggctcagaa aaaagcattg ttgtcaaaac tgaataaaga
     2281 actccgcaaa ggcgcggatg agtcagaaac cctcagacaa ctggaagtac ttcagaaaaa
     2341 cagtgcggaa gaacctgtga ggtataaatt catttttaat gatgccacga ctgcggcaat
     2401 taagaatcag ctttgtggta aatggcgttc agttggtatt atgtcagatg aagccggtat
     2461 tatatttgat ggatataccc tgtctgagtt gccttttatt aacaaaatgt gggatggctc
     2521 tgtattgtct gtggacagaa agaatgaacc cgaacagatg attgaaaatg ccagaatgac
     2581 gttatccctc atggtgcaac ccggtctttt tgaccgatat atggagcgaa aaggttctgt
     2641 tgccagggac agtggttttc ttgcccgttg tcttatcagt aaacctgcaa ccacgcaggg
     2701 gaaacgcttt attaacggag ctgtgattcc gggaggatca ttaacggcat ttcatgaacg
     2761 attaatggaa ctggccagag gcagtattga aaaatccagt gaggatgaac ggtattgtct
     2821 gcatttttcg cctgaagcgc aaaaaatatt tattgagcac tataatgttc tggagcagga
     2881 tttatcccct tccggaccct taagcccgtt tcggggacat gtttcaaaaa aaacagaaaa
     2941 cattgcaaga attgcagcgt tatttcagta tttcagcta
//
LOCUS       DAZFRG010000381         2972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFRG010000381 DAZFRG010000000
VERSION     DAZFRG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.383"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(512..1369)
                     /gene="repA"
                     /locus_tag="ACHKUC_004845"
     CDS             complement(512..1369)
                     /gene="repA"
                     /locus_tag="ACHKUC_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO6620924.1"
                     /translation="MTELQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(1362..1436)
                     /gene="tap"
                     /locus_tag="ACHKUC_004846"
     CDS             complement(1362..1436)
                     /gene="tap"
                     /locus_tag="ACHKUC_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO6620925.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCD"
     gene            complement(1670..1927)
                     /locus_tag="ACHKUC_004847"
     CDS             complement(1670..1927)
                     /locus_tag="ACHKUC_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO6620926.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPAKERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            complement(2229..2927)
                     /gene="aphA"
                     /locus_tag="ACHKUC_004848"
     CDS             complement(2229..2927)
                     /gene="aphA"
                     /locus_tag="ACHKUC_004848"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6620927.1"
                     /translation="MKKQNLALISCLLFSSLSFAATDTGELKAGATLSELTEQYPVHW
                     VSVEQVIQGLKSAPLNVGFDIDDTLLYSSPGFFHGKQKYSPESDDYLNKQEFWNEVSG
                     GWDRFAIPKKSAIKLIKYHMERGDNIYFITGRPAPDNGKEDVTATLRDYFKIPEGKLN
                     KVIFAGTDKDAKVKYIKNNGISVYYGDSDNDILDARKAGAEGIRVLRPLNSTNSTEPR
                     NGQFGERVIINSQY"
BASE COUNT          735 a          716 c          697 g          824 t
ORIGIN      
        1 gggccggggc ggggccgctt ttcagttatg agggaggggc tttgtggttt cggttctgcg
       61 ctggtccggg gtttttctgg aggttgtttt tgtgtgttgt aactaaagtg gctccggtcg
      121 gggcccgccg cttgcggtgg gaggtgcata tttgtctgtc cacaggacag gcaatgaata
      181 ggttttcttt ttaaatgagt gtaattaagt agtttaaagg agatataaac aggtgtttaa
      241 aagatacatt gcaccctgta agactggcgg ctggcgcttt atgatatgaa cggttgtaac
      301 cttatgggga agtcccttgc agtttaatgt ggataagcaa aattccccgt ctgtgaggcg
      361 tgttttgtat cgaaaacagg gggggccgga tgcaccagaa gctggatgat gaggttgttt
      421 tttgtatgtg gtgctgattt tttgtgcgct gtcgggcttc agacgtgcgg aagcctccgg
      481 cgcaggccgg attattctga ggaggtcact ttcaggggga agctgtggcc agccggctgt
      541 aattacggtt acgtgacaga atcatgcgct ccttcacacg acgctccact tcgcgtttta
      601 ccgcctcacc attagcagtg aagcgccctt ccgagatttc acgcgtcagc tgccgtttca
      661 ccagggtgac gatatcctga cgttccctgt tcgcatcacg acgcgcacgg gcacgcttta
      721 ttccccggga cttaagctct gtctgataac tgcgaaaacg ctcacgaaca aaacgccagg
      781 ctttcgctat cagttcatcc atgcccaggg tatccagccc ctgctttttg cgctgtctgt
      841 tttcccattc aacacgactg cggcgcgcag ctgccaccgc atcctcagac acatcgagag
      901 cggcaaacag tgccggtgtg aacgtgatgt cggtcggaat gtagcagccg ataagcgggt
      961 catattccgt ctggtaggta atcaacccca gctcagccag gaacgtcagg gcacgggtgg
     1021 cacgggtgat ggagagtttt ccggcaccgg actctgtcgc cagtccgcac tcaatggcca
     1081 gcgtggtgat ggagcactgg acgcggttgg ccagcgggtc atagtggaaa cacagcccct
     1141 gcagcagcgc atcaatggca cgtcgacgca gcaccggtgg catgcgccgg cgcaaaccac
     1201 gggaacgggc atgcgccaca tgaatggcga aatcaaaacg ggaggtgaag cccaccgcct
     1261 tttccatcag tttttcgcag aacttcagcg ttccggcacc tttacggggt gtaaacaccg
     1321 gattcgggtt ctttacctgg cggtaatacg tttgttgaag ctcagtcaca ccatcctgca
     1381 cttacaatgc acagaaggag cgagcacaga aagaagtctt gaacttttcc gggcatataa
     1441 ctatactccc cgcatagctg aattgttggc tatacggttt aagtgggccc cggtaatctt
     1501 tctgactcgc caaagttaaa gaagattatc ggggtttttg cttttctggc tcctgtaaat
     1561 ccacatcaga accagttccc tgccacctta cggcgtggcc agccacaaaa ttccttaaac
     1621 gatcagcaat ctaacatcgc agtactgata cagcaagaaa cgcaaatatt cacaaagatt
     1681 ttctgcgttt cacctctgac tctatcagcc actcaagcat ctgtgcctga gtgatcccgt
     1741 cttcatcagc aagctcactt aacttttctt tcaggcgaag ctgaatgacg gcatgaaacg
     1801 ctttatgagt ggcactcctt cttgcgagag acgctttttg tcgttctttc gccggaacag
     1861 gattacccct tctgtaaggt cgcttttttc ccgatgagga agtctctgca tttactgtct
     1921 gcgacatctt gcctcctctc ttctcagaca gggatcgttt cgcagaggat actacagttt
     1981 tttgaaatca gcgacttgag aattgtgatc atgatctgtg ttggcgcaca aatcatctcg
     2041 gattgtggag atttaatgtg atttttgttg cgaaatagct aaaaacaagg agaaatctta
     2101 actgctgtgc tgcctgccag gagaagccgg cagcacttga gatattaagc acaggcagat
     2161 atctgaatca gaaaggcaaa gatacctgcc aggatgttat cactattaca aataacaata
     2221 atgctctatt agtattgtga attaatgatc accctctcac caaactgacc attacggggc
     2281 tcagtgctgt ttgttgaatt taatggccgg agaaccctaa tcccctcagc accggctttt
     2341 ctggcatcaa gaatatcatt atcagaatcc ccataataaa ctgatatgcc attatttttt
     2401 atatacttca cttttgcatc tttatctgta ccagcaaaaa taaccttatt cagtttccct
     2461 tcaggaattt taaaataatc tctcaaagtt gctgtcacat cctctttccc gttatcagga
     2521 gcaggtcttc cggtaataaa gtaaatatta tcaccccgtt ccatatggta ttttattaat
     2581 tttattgcgg attttttggg tatagcaaaa cgatcccaac caccacttac ttcattccag
     2641 aactcttgct tattaagata gtcatcactt tctggtgagt atttttgctt tccatgaaaa
     2701 aaaccaggac ttgaataaag caaagtgtca tcaatatcaa agcccacatt cagtggtgcg
     2761 gatttcaatc cctgaatcac ctgctcaaca gatacccaat gtaccgggta ttgctccgta
     2821 agctctgata atgttgcccc tgcttttaac tcgccagtgt cagtcgctgc aaaagataat
     2881 gagctaaaaa gcagacatga tattaatgcc agattttgtt ttttcataat aagaatctct
     2941 ttcaaaacta aatcctaata ctatttccct gc
//
LOCUS       DAZFRG010000382         2917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFRG010000382 DAZFRG010000000
VERSION     DAZFRG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.384"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..1543
                     /gene="gltP"
                     /locus_tag="ACHKUC_004849"
     CDS             230..1543
                     /gene="gltP"
                     /locus_tag="ACHKUC_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO6620928.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(2257..2916)
                     /gene="yjcO"
                     /locus_tag="ACHKUC_004850"
     CDS             complement(2257..2916)
                     /gene="yjcO"
                     /locus_tag="ACHKUC_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO6620929.1"
                     /translation="MTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADSWFSSGDLSKA
                     EYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSKEGEVTLAHIL
                     VNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKADDDKATWYFK
                     RSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDTGCEEFEKLTN
                     G"
BASE COUNT          614 a          734 c          785 g          784 t
ORIGIN      
        1 tctataacaa atggttattc attaatcctg ccataaactc gcgttattat gcattaatgc
       61 agcgaaaagc tctgttgtta aagggttgcg caacataccg cgcaaatgat actgatcata
      121 agcgttaaaa aaatctacaa accaacgcaa cacaattcat gccctggcag tatgtcacgt
      181 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
      241 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
      301 ctacctgcac taccatagcg acagccgcga ctggctggtt gtcaatttgc tctctccggc
      361 gggtgatatc ttcatccatc tgatcaaaat gattgttgtg ccgattgtga tctccacact
      421 ggtagtgggt atcgcgggtg ttggtgatgc caaacagctc gggcgtattg gtgcgaaaac
      481 cattatctac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
      541 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
      601 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
      661 gtcgctggtg ccgacgaaca tcgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
      721 cttcttctcg gtgctgtttg gtctggggct ttcatccctg cccgcgacgc atcgtgaacc
      781 gctggtgacc gttttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
      841 gcgttatgcg ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
      901 ctcttctctg tggccactgg cgaaactggt gctgctggtg cattttgcca ttctgttctt
      961 cgcgctggtc gtactgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
     1021 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtgct
     1081 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagtttcgt
     1141 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
     1201 tattttcatc gctcagctgt atggcattga cctctccatc tggcaggaaa tcattctggt
     1261 gctgacgctg atggtgacct cgaaagggat tgctggcgtg ccgggcgtgt cgtttgtggt
     1321 gttgctggca acgctgggca gcgtagggat cccgctggaa ggtctggcgt ttattgccgg
     1381 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
     1441 ggtgctggtg attgccaaat gggaacacaa atttgaccgt aagaaagcgc tggcttatga
     1501 gcgtgaagta ctgggcaaat ttgataaaac tgcggatcag taattgaaga ttgccggggg
     1561 gatatccacc cggcaatgtg tggatgcctg actgagtaat agcctgatgt agcgtgattt
     1621 ttgtaggtcg gataaggcgt ttacgccgca tccgacatca acgcctgatg cgacgcttgc
     1681 cgcgtcttat caggcctacg ccagacagcg caatagcctg atgtagcgtg gtttttgtag
     1741 gtcggataag gcgtttacgc cgcatccgac atcaacgcct gatgcgacgc ttgccgcgtc
     1801 ttatcaggcc tacgccagac agcgtaatag cctgatgtag cgtgattttg taggtcggat
     1861 aaggcgttta cgccgcatcc gacatcaacg cctgatgcga cgcttgccgc gtcttatcag
     1921 gcctacgtca gacagcgcaa tagcctgatg tagcgtgatt ttgtaggtcg gataaggcgt
     1981 ttacgccgca tccgacatca atgtctgatg cgatgctgac gtgtcttatc aggcctacgt
     2041 cagacagcgc aatagcctga tgtagcgtgg tttttgtagg tcggataagg cgtttacgcc
     2101 acatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct acgtcagaca
     2161 gcgcaatagc ctgatgtagc gtgattttgt aggtcggata aggcgtttac gccgcatccg
     2221 acatcaacgc ctgatgcgac gtttgctgcg cctaccttaa ccgttggtta atttttcaaa
     2281 ctcttcacac ccggtatcaa acccttccat acagctcagg ttcaaccagt gcagcgcctt
     2341 ttgtttgttc ttctcgataa aaccctcttc accgtttaag aacatcattc ccgcccagta
     2401 ttcggaataa ccggttcggg aaatcgcgga gctacgtttg aaataccagg ttgctttgtc
     2461 atcgtcggcc ttaatgccca cgccgttggc gtaaatcaaa ccaagcagca tttgggcatc
     2521 gacggcagag tcgttctcca gatcttccga ggcgttttct aacagcgaaa tcgcctttgg
     2581 ataatccggt ttacccgctt gcgtatttac cagaatatgc gccagcgtta cttcaccttc
     2641 tttactgccc gcttgcgctg ctttctcggc aagcactttt gcttgtggat agtccagact
     2701 gaccggattg gtgattttga tctgcgccaa cagcgcgcag gcatcggcat caccgctgtc
     2761 ggcggctttc tgtgcccaat attcggcttt gctcaaatcg ccggagctaa accagctgtc
     2821 ggcaagaaaa tattgtgcgc gtcggtctcc ggcctctgct gcctttaaat actggctgcc
     2881 aggctcggag tcgttcgcgt gggcaaagaa tgtcaaa
//
LOCUS       DAZFRG010000383         2909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFRG010000383 DAZFRG010000000
VERSION     DAZFRG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.385"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2407)
                     /locus_tag="ACHKUC_004851"
     CDS             complement(<1..2407)
                     /locus_tag="ACHKUC_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO6620930.1"
                     /translation="MNIPALNFDHSHHKLKIRGLQSPVDVLTFTGHEQLSSPFRYDIQ
                     FTSRDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARY
                     EVRLEPRMVLLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFFDDQSGYERGLTLPLRHPSGL
                     HDSATEAVWGLNTAYSVVEKSVTTRDYNYREATAEMTTGQHDATGGDKTTYGEAYHYA
                     DNFLQKGDKEVAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADTQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGV
                     AIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAQKSIVITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIESGEFNAKFLFTDDDGLPYANTKYIACFSDGTQ
                     KEGI"
     gene            complement(2664..>2909)
                     /locus_tag="ACHKUC_004852"
     CDS             complement(2664..>2909)
                     /locus_tag="ACHKUC_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620931.1"
                     /translation="RSLFDIMKFRVGICSSRLGGDYLFQKDNGYCASEDEDSINIEYF
                     LWRFGLNGGIEKMYFNIRTNQVVIDIDGSDVHSLYDN"
BASE COUNT          589 a          834 c          740 g          746 t
ORIGIN      
        1 taataccttc tttttgagtg ccatcagaaa aacatgcaat atattttgtg ttggcataag
       61 gtagaccatc atcatcagta aaaaggaact tagcattaaa ttccccgctc tcaatcactg
      121 gaaacgccat caactccggt gtcagcttcg ccttcggctt acgcccataa tatcccgcct
      181 taacgttata ttctcccggc gttcccgctt ctattccgca ggcatccagc cggatgtagc
      241 tgccaccgcc gttaagggtg attttcttct tcgcggtgat cttgatctca tcttccgtac
      301 tggtgatcac tatcgatttc tgtgccaaca gttccatcag gtcgttctgt gcctgtaccg
      361 agatcgcccc cttgttggca aacagtttga tgcccgcttt acgcacaaac acgctcagcg
      421 cctgacccac accgataaac atattcttca caacaccgat atcagcgtga tttcctgcgt
      481 tcgcgatcag gtttttcgat gcggccagtt gcaggtgctc tccgctggcg atcgccacgc
      541 ctttgggtgc gcttagcagg atcgccgcct gcgtcagatc ttttacactc tgctgcaaca
      601 gcgctatttg tgatgacaga tctgccggat cggcattcgc cgtctgcgca tcagtggaca
      661 gcgacttcat ctgtgactgt gcgccatcca gtattgccat cgccggagcc atttcgcgca
      721 cctgaccctg cgccttgtcc tgcgtatcgg cgctgataaa aatccctttc ttcgcccgta
      781 ctgcccccca catatcggta cgcagttcaa agccttcgcc gcgctgctgt ttcccggcat
      841 ccaccagatg accgaggttg agctggctct tgccgccgta ctcggtactg accttgatat
      901 gctctttgcc gcgctcatcg tcgaggcgga ttttgttgtt cgccggggtt cgcagcacgt
      961 tgcgcttgta gttctggatg gtcacatggt cggtgtgcgc cgagtcgtgc tttaccccgg
     1021 cgatatacgg cctgtccggg ttgccttcct caaaggcgat gctgacttcc gttcctgcca
     1081 gcagcggcag gtgcaggccg taggtatccc cggcataggg cctcgactgg cgtacccaca
     1141 ggctctcata gcccggtttc cacgtatcgc ggtcaaagtc gaggttgacc cggtagcgcc
     1201 cgtccttgtc gatatgggcg tagatgtcgt tcttcaccgt gctggtgacc cgcgccggga
     1261 gcgtgccggc catcaccggg cgggggataa gcgccgggcg gtagccgtag cgctctgagt
     1321 acggtatcgc cgtgaacgtc agttcatagc tgcggtcacg cgccgcgctg gccgtcacgc
     1381 cggtaatcag cacgcccttg cggaacaccg caggcgcgtc gtcgccctga accctgattt
     1441 ccagccccgg catcagcagt gaactggtgc tctggccttt aagaatggcc tgttcattca
     1501 ggtaacgttc gtgacggata cgggcgtaaa acgcgccgct ttccgctacc tctttatcgc
     1561 ctttttgcag gaagttgtcg gcatagtgat aggcttcgcc gtaggtggtt ttatcgcccc
     1621 cggtggcgtc atgctggcct gtggtcattt ccgccgtggc ttcacggtag ttgtagtccc
     1681 gcgtggtgac gcttttttcc accacgctgt atgcggtgtt cagcccccat acggcttccg
     1741 tggcgctgtc gtgcagccct gaaggatggc gaagcggcag cgtcagtccg cgctcatagc
     1801 cgctctggtc gtcgaaaaac tcgataacct caattttcag ccgcgcgtca gtggcgaagc
     1861 ggaaccagat acccacttcc gacagcaggc gtgaaataaa tgtcaggtcg tcctcgccat
     1921 actgcatcac ctgttcgcgg gccgggtatt cgcttttgag gttaaacacg aagtcctgac
     1981 cgcgcatctg gtgccgctcg cgcaggattt tttcgacgat ttgcggcacc gtcagattct
     2041 ggtaaatggc gttctgacgg ctgcgggcca gcagcaccat acggggttca aggcgcactt
     2101 cgtaacgggc ttcgtcctgt gacgatgaga gatgtttaaa gccggtaatg acgccgtgca
     2161 gggtgcgcag ggcagtctgt accggcattc cctgtaccgg cggtgccgtc aggctgaatg
     2221 cgccgtcctg catcagcacc gactccggcg ttattgcttt atcgcggctg gtaaactgaa
     2281 tgtcgtagcg gaagggggag ctgagctgtt cgtggcctgt aaaagtcagc acgtccaccg
     2341 gactttgcag gccacggatt ttgagtttgt ggtgtgagtg gtcaaagttt agggcaggaa
     2401 tattcatcgt tattttcctg tatagataga aatactctca tcattcaaaa taaatcaaca
     2461 atgaaactca atgaactctc gccattgtgg tgtcacatta tttctcacta agaaatcgtt
     2521 tctttcatca gacgatagac tgtgccagaa cggagaccag atatcgccgt gaacgtcagt
     2581 tcatagctgc ggtcacgcgc cgcgctggcc gtcacgccgg taatcagtac gcccttgcgg
     2641 aacaccgctg gtgcgttgtt gatttagtta tcgtacaatg agtgaacatc actcccatca
     2701 atatcaatca caacttgatt agttctaatg ttaaaataca ttttctcaat ccctccattc
     2761 aaaccaaatc gccacaaaaa atattcaata ttaattgaat cctcatcttc agacgcacaa
     2821 taaccattat ctttttgaaa aagataatct ccgcctagtc gactactgca tatgccaacc
     2881 cgaaacttca ttatatcaaa caacgatct
//
LOCUS       DAZFRG010000384         2899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFRG010000384 DAZFRG010000000
VERSION     DAZFRG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.386"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..599
                     /locus_tag="ACHKUC_004853"
     CDS             <1..599
                     /locus_tag="ACHKUC_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620932.1"
                     /translation="SVFRKPHLSISMIVFLVCFCLFGANWPINGLLPSYLADNGVNTV
                     VISTLMTIAGLGTLTGTIFFGFVGDKIGVKKAFVVGLITSFIFLCPLFFISVKNSSLI
                     GLCLFGLMFTNLGIAGLVPKFIYDYFPTKLRGLGTGLIYNLGATGGMAAPVLATYISG
                     YYGLGVSLFIVTVAFSALLILLVGFDIPGKIYKLSVAK"
     gene            611..1729
                     /gene="nanY"
                     /locus_tag="ACHKUC_004854"
                     /gene_synonym="yjhC"
     CDS             611..1729
                     /gene="nanY"
                     /locus_tag="ACHKUC_004854"
                     /gene_synonym="yjhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,7-anhydro-N-acetylneuraminate hydratase"
                     /protein_id="HFO6620933.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            1772..1897
                     /gene="ythA"
                     /locus_tag="ACHKUC_004855"
     CDS             1772..1897
                     /gene="ythA"
                     /locus_tag="ACHKUC_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YthA"
                     /protein_id="HFO6620934.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(1950..2207)
                     /locus_tag="ACHKUC_004856"
     CDS             complement(1950..2207)
                     /locus_tag="ACHKUC_004856"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFO6620935.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            2230..2433
                     /locus_tag="ACHKUC_004857"
     CDS             2230..2433
                     /locus_tag="ACHKUC_004857"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6620936.1"
                     /translation="MDITPKYVGIASGLMNAGSAVADIISPIAFGIIIDKTGNWSLPF
                     YGSVALLVIGIFLTFFMRPDKSL"
     gene            2521..2899
                     /locus_tag="ACHKUC_004858"
                     /pseudo
     CDS             2521..2899
                     /locus_tag="ACHKUC_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          860 a          541 c          634 g          864 t
ORIGIN      
        1 tgtctgtctt cagaaaacca catctttcaa tctctatgat cgttttcctc gtctgttttt
       61 gtctatttgg tgcaaactgg ccgataaacg gactacttcc ttcctacctg gcagataatg
      121 gagttaatac agtggtcatt tcaactctga tgacaatagc aggtttagga acactgacag
      181 gtacaatatt ttttggtttt gttggtgata agattggtgt aaaaaaagcc tttgtagtcg
      241 gtctaataac ttcatttatt ttcctttgtc ctcttttttt tatttctgtg aaaaactctt
      301 ctcttatagg attatgtctc tttggattaa tgtttacaaa tttaggtatt gcagggttgg
      361 ttccaaaatt tatatatgat tactttccaa caaaattaag aggattaggg accggtctta
      421 tttataactt aggggcaact ggaggaatgg ccgcacctgt attagctaca tacatttcag
      481 gatattatgg cttaggtgtt tcattattca ttgttacggt tgcattctct gccttattaa
      541 ttttgttagt tggttttgat attccaggta aaatttataa actatccgtg gctaaatgat
      601 tggaggcttt atgattaatt atggcgttgt tggtgttgga tactttggcg ctgaattagc
      661 tcgttttatg aatatgcatg ataatgcaaa aattacatgt gtatacgatc ctgaaaatgg
      721 agaaaatatt gcccgtgaat tgcagtgtat caatatgtca agcttggatg ctttagtctc
      781 aagtaaatta gtcgattgcg tgatcgtagc caccccaaat tatctgcata aagaaccagt
      841 aattaaagca gcaaagaata agaagcatgt tttttgtgaa aaaccaattg cattaagtta
      901 tgaagattgt gtggatatgg tcaaagcgtg taaagaagct ggtgtgacct ttatggccgg
      961 gcatattatg aattttttca atggggttca atatgcacgg aagttaatta aagaaggtgt
     1021 tatcggcgaa atattatcat gtcatactaa gagaaatggc tgggaaaaca aacaagagag
     1081 actttcctgg aaaaagatga aagaacaatc tggtggacat ctatatcatc atatacatga
     1141 gttagattgt gttcagcatt tacttggaga aataccagag acggttacta tgattggtgg
     1201 aaatttggcc cattctggtc caggatttgg caatgaagat gatatgttat ttatgacctt
     1261 ggaattcccg tcaggaaaac tagcaacctt agagtggggg agtgcattta actggccgga
     1321 acattatgtc atcatcaatg gaactaaagg ctctattaaa attgatatgc aagaaacagc
     1381 agggtcactt aggattggcg gtcagacaaa gcattttttg gtccatgaaa cacaagaaga
     1441 agatgatgat cgtcggaaag gcaatatgac ctcagaaatg gatggcgcta tagcatatgg
     1501 tcatccagga aaaaaaacac cattatggct tgccagttta ataagaaagg agacgttatt
     1561 cctccataat atcctctgtg gtgcaaaacc tgaagaagat tatattgacc ttctcaatgg
     1621 tgaggcggcc atgtcggcga ttgctactgc tgatgctgcc actctttcaa gatcgcagga
     1681 caggaaagtg aaaatcagtg agatcattaa acatacatca gtaatgtaaa aactatacgc
     1741 ctcttgattt aattcaggag gccttttatg tatgatcaag aattttatct tcgataatct
     1801 cataatttta gcagtgccat ttatgataaa aacatccctt aaaaccaatc taatcttttt
     1861 cttcctctgt gtatttgttc cccatatggc gtcataattt acgctacgta atacgggata
     1921 gttacgatac gcagcgatag cgctaagttt tagttaaaat cccccatcag cgggatatgg
     1981 ggatctctgg gaggttcagg ttttagcatc gcgactttaa tacgacagac gctggtgacg
     2041 atgatgccaa gtagtgactc ttccgaataa ggttcccact gaccaattgg cgcgcgcttc
     2101 gcaataaaat ctcccttcgc ccagtattgg gaaaagtaga tacattcaaa ctgtgtacgc
     2161 tgtttcgtct cacagttcac gacattgctg gccgatgagc tgaccattgg gcctatccgg
     2221 gctgtcccga tggatattac gccgaagtat gtgggaattg ccagcggatt gatgaacgcc
     2281 ggttccgctg tcgccgacat tatttcacct atcgcctttg gcattattat cgacaaaaca
     2341 ggcaactgga gtttaccatt ctacggttcc gtcgcattgc ttgttattgg tatattcctg
     2401 acgttcttca tgcgtccaga taagtcattg taaaagtcga atcagggctg aagtggcaca
     2461 ctgaatttgg ccacctgaac agaggtgata tgctcacctc agaacaacac aggtgctcca
     2521 atgaaaaaaa gaaatttcag cgcagagttt aaacgcgaat ccgctcaact ggttgttgac
     2581 cagaaataca cggtggcaga tgccgcgaaa gctatggatg ttggcctttc cacaatgaca
     2641 agatgggtca aacaactgcg tgatgagcgt cagggcaaaa caccaaaagc ctctccgata
     2701 acaccagaac aaatcgaaat acgtaagctg aggaaaaagc tacaacgcat tgaaatggag
     2761 aatgaaatat taaaaaggct accgcgctct tgatgtcaga ctccctgaac agttctcgat
     2821 aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt tcggggttca
     2881 tcgcagcagc tacagatac
//
LOCUS       DAZFRG010000385         2859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFRG010000385 DAZFRG010000000
VERSION     DAZFRG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.387"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..1238
                     /gene="mtlD"
                     /locus_tag="ACHKUC_004859"
     CDS             90..1238
                     /gene="mtlD"
                     /locus_tag="ACHKUC_004859"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6620937.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAEISGLDANSEVVSEAVTAYKAMQ"
     gene            1238..1825
                     /gene="mtlR"
                     /locus_tag="ACHKUC_004860"
     CDS             1238..1825
                     /gene="mtlR"
                     /locus_tag="ACHKUC_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO6620938.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1836..2045)
                     /gene="yibT"
                     /locus_tag="ACHKUC_004861"
     CDS             complement(1836..2045)
                     /gene="yibT"
                     /locus_tag="ACHKUC_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO6620939.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            2329..2691
                     /locus_tag="ACHKUC_004862"
     CDS             2329..2691
                     /locus_tag="ACHKUC_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO6620940.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          771 a          702 c          713 g          673 t
ORIGIN      
        1 aaagcccctc tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
       61 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
      121 tcggtcgtgg ctttatcggt aaactgctgg cggacgcggg tatccaactg acgtttgccg
      181 atgtaaatca ggtggtgctt gatgccctga atgcccgtca tagctatcag gttcatgtgg
      241 ttggtgaaac cgagcaggta gataccgttt ctggcgtcga tgccgtcagc agcattggcg
      301 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggccctgtcg
      361 tgctggaacg tattgctccg gccatcgcca aagggctggt gaaacgtaaa gaacaaggta
      421 atgaatcccc actgaacatc atcgcctgtg aaaacatggt ccgcggcacc acgcagctga
      481 aaggccatgt gatgaacgcc ctgccggaag atgccaaagc gtgggtagaa gaacacgttg
      541 gctttgttga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
      601 cgctggaagt gacggtagaa accttcagcg aatggattgt cgataaaacg cagttcaaag
      661 gcacgctgcc aaacatccct ggcatggaat taaccgacaa tctgatggca tttgtcgaac
      721 gtaaactctt caccctgaat acaggtcatg ctataaccgc gtacctcgga aaactggccg
      781 gtcatcagac cattcgtgac gcaattctcg acgagaaaat ccgcgcggtg gtaaaagggg
      841 caatggaaga aagcggcgcg gtactgatca agcgctacgg ctttgatgct gacaaacatg
      901 cggcatacat ccagaaaatc ctcggtcgtt tcgagaaccc gtacctgaaa gatgatgtag
      961 aacgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac
     1021 tgctcggaac cctggaatat ggtctgccac ataaaaacct gattgaaggt attgccgctg
     1081 caatgcactt ccgcagtgaa gatgacccgc aggctcagga actggcagcg ctgattgccg
     1141 acaaaggccc acaagccgcg ctggcagaga tttccggtct tgatgccaac agcgaggttg
     1201 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggatac
     1261 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
     1321 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
     1381 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
     1441 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgatggtc cgctgggcga
     1501 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
     1561 agatgcggaa ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc
     1621 ttttaccgac gacgaaatac ttggaccctt tggtgaactg cattgcgtgg cggcgttacc
     1681 accgccgcca cagtttgaac ctgcagactc cagtttgtat gcaatgcaaa ttcagcgtta
     1741 ccaacaggct gtgcgatcaa caatggtcct ttcactgaca gagctgattt ccaaaatcag
     1801 cttaaaaaaa gcctttcaaa agtaagtact ttcgcttact tctcctctac ctgtttcgga
     1861 cgataaagct gacgataata ctccagacgt tgtagatata acggcacgct ttcatcaggt
     1921 attccggacg gaatcgcgtt acggggaggt agttttttca gatactcccg gaacgcctgg
     1981 ctggatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg
     2041 cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca attttagttt
     2101 agatcccgat ataaatgaca ttaaggaaaa cgtgctcaat cctcaataat cacctgcttc
     2161 tgttgactat cttttttctt taaatttctt aactaatcaa tgtattgggt gattttttaa
     2221 cgcattaatg gttacaatct ggaactatta caatgccatc agttgtgaac aaagcacctg
     2281 gtcgcgcata ctagaggcta taaatttatc tctatcagaa gtcatcacat gaaagaagtc
     2341 gaaaaaaacg aaatcaaacg tctcagcgat cgtctggacg ccatccgtca ccagcaggcc
     2401 gatctatcgc tggttgaagc cgcagacaaa tatgccgaac tggaaaaaga gaaagccacg
     2461 ctggaagcag aaattgctcg cctgcgcgaa gttcacagcc agaaactcag caaagaagcg
     2521 cagaaactga tgaagatgcc gttccagcgt gcaatcacca aaaaagagca ggctgatatg
     2581 ggcaagctga aaaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggc
     2641 cgcgaaatgg gcctgcaaga gatgacaggg ttctcaaaga ctgcgtttta agttcctttc
     2701 tcgtcgctgt aatcacagcc tctcaccatt tactcgctcc tgctggcaca tgcaggagcc
     2761 tgtttcagag atcgaatctt tataacgttc ttaacattat attcaactcc acctacactt
     2821 ctgagcgatc gtagcaggct aaattctgaa aatcaggta
//
LOCUS       DAZFRG010000386         2854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFRG010000386 DAZFRG010000000
VERSION     DAZFRG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.388"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2814)
                     /gene="yfaL"
                     /locus_tag="ACHKUC_004863"
     CDS             complement(<1..2814)
                     /gene="yfaL"
                     /locus_tag="ACHKUC_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO6620941.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NSNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGVAGNTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTP"
BASE COUNT          695 a          789 c          606 g          764 t
ORIGIN      
        1 cggcgtggga tcaggatctg gagtcgggtc tggatcaggc ggcggcgtcg gattgacttc
       61 ttgtgatcgc agataccagt cgttgttatc ttccaccagc gtgtagtcat acgcccccat
      121 attgacgtag ccgcttcctg ccagactgaa ctgcgcattg ttttgaaact gcgcgggatc
      181 agctgcgaaa tcaactactt taatacctgt agatgtcggc tcaccaatac ccgtaatggg
      241 gttaattacc acggccgtgt tgccagcagt attaccgttc aataccagtt ggtcacttgc
      301 tgagtcatcg ccgtttaatt cgctatcgag cagtagcgta ccgccgccgg tataatcacc
      361 atttaccgtc agcgtgttgc cagcgacgcc attttgcaga ctgacagcac cggacgtatt
      421 ggtcagattg ccattaaccg tcacgtcgga aagataagtc aggctgtcag tttcaaggtt
      481 ggtattaagc tcacctgcca gcgtcacgcc atcggtggca ttcaccagcg taccgtcgcc
      541 gctaaacagc gaggcatcca gcgcctggtt cgtatcctgc tgcgtcaatc gcaacacagt
      601 accatcggtt atgtcgatat cgccgccaga ggtggcatcg attgaacgaa tatcctgatt
      661 ttgcccggtt tcaaaaacac cgtcttcccc cacccacagt gaagaaacat aaggaataat
      721 attctccgct tcacctttta gcgttccgtc ctccacacgt accgcagacg tcgcagtgcc
      781 gctggcggtc aactccagcg tcccgccgcc acttttagtc aacgtgctta cgctatccag
      841 ttgttgcccg gtactatccg ccatcaaccc gccccattgc gtatctacgc catcctcaac
      901 gtgtatttcg ccatcagcgc gcatttcaat attcctgccc tgacctgttg tagccgagcg
      961 agtgtcaaca tcagtcgtca catccatttc actgtgttgt tggggatcag tgaagatcac
     1021 tgaacggttg taacttgtgt cccccaattg cgaattatcg ctgacttcaa gcgtaccgga
     1081 ggcgatttgc gtattaccga gataaccgtt gctattcgcc agcgttacgc gctgatcccc
     1141 cgcattaatg acgacataaa cgccattctg gctggcatca ctgccgccaa tatgtaaatc
     1201 atcgccactg ctgatatcaa cgacatcatt actgccaatc actgtccccc cgctgccgct
     1261 gatttgcaga ccgtcattgt atgagctatc gccatcccag gtggtgaaat cagagagatc
     1321 caatactccg ccatttagca cgatcgattg cggatcgctc tgcattgccc gtaaatcggc
     1381 ctgattcccc gctagcgaca atacggcgtt atcttccacc acaatatcgc cggttaatgc
     1441 catcgactgc gcccctgtca ggagatagct gccatcttgc gctaccgtca actgtccttc
     1501 accctgaatc gagccggaaa aaccgccctg gtttactgta acattgccgc ctgcgtcgat
     1561 attcaaaatg ccattagcaa aaccggttaa tgagtgcaca aacgtttgtt gtgtattgcc
     1621 aacattcagt tctgcctgat actcagaatg aacagtgctg ccgaccatca gaccaaagca
     1681 atcctgcgga tcgctctgac agtgcgtgtc gccgacattc attaactcat cgctgcgccc
     1741 caacgtcacc tcgccattct ggatactcat ctcgccggta aacgcattat tgtcggcatt
     1801 gaggactaac tctcccgctc cctctttaac gatgactccc gttccggcaa gagaatcaat
     1861 cgcgccatcg ttgctggtat tgcctattac cagcgtttta tcggcggcaa tgttaaattc
     1921 agccacacta tgaccgatat acataaagcc ccccgccgct gccgtcgccg aatgatcata
     1981 tcccgaggcg ctgttatttt catcaatcat cacaccattg ttttggtcat aattatcatc
     2041 aacagaaata tcaataagat acggggatgt ggtgctgctg gtataaattg ctccgccata
     2101 accttctgca tagttattgg taaaggaggt attattaatt atcgtttttc ctgaaagatg
     2161 tgtgttatcc gtactattat ccgtaacatc aatggcgccg ccatccccat cactataact
     2221 tgttgatgta tatgcttggt tattattaaa agcatcatca cttaaataga cgtcattatt
     2281 gatggtataa attgcaccgc ctttgccgtc attagcgatg ttattgtgaa atacggcgtt
     2341 agtgacacgt aaatcggcgg caccggtatc attggtaccg gcagaataga ttgctcctcc
     2401 atagcctcct gcaacattac tgtcgaaaat aacattcgca agatttatcg ttgagttttc
     2461 tttagcaaag atagccccac cattattata ttcgccacta accgagttat cagcaaacag
     2521 ggtcatccct gtttcatttt gtggtgatat cgtaaattca gcactttgct gcaaaaatac
     2581 tgcgccaccg ctggcattat tggccatacc cgaaaaaatc aaccattgcc catccgcgat
     2641 gctccagtcc tgcatattgc ctgaaaggct ttgcctgctg gcctgacagc tcgctttgat
     2701 atcatatccc tggcatgaat caatgaccgc tgcggcaccg ttaccagaga aaagagatgc
     2761 aatcattgac gggagtaaag ataaatactc cttgcgtaga aagataatcc gcatattcat
     2821 ctaaccatca ttttctataa gatggcgtat ttaa
//
LOCUS       DAZFRG010000387         2799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFRG010000387 DAZFRG010000000
VERSION     DAZFRG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.389"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..120)
                     /locus_tag="ACHKUC_004864"
     tRNA            complement(44..120)
                     /locus_tag="ACHKUC_004864"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Asp,seq:gtc)
     gene            complement(253..984)
                     /gene="dnaQ"
                     /locus_tag="ACHKUC_004865"
     CDS             complement(253..984)
                     /gene="dnaQ"
                     /locus_tag="ACHKUC_004865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO6620942.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1049..1516
                     /gene="rnhA"
                     /locus_tag="ACHKUC_004866"
     CDS             1049..1516
                     /gene="rnhA"
                     /locus_tag="ACHKUC_004866"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO6620943.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1513..2235)
                     /locus_tag="ACHKUC_004867"
     CDS             complement(1513..2235)
                     /locus_tag="ACHKUC_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6620944.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2269..>2799
                     /gene="gloB"
                     /locus_tag="ACHKUC_004868"
     CDS             2269..>2799
                     /gene="gloB"
                     /locus_tag="ACHKUC_004868"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO6620945.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALS"
BASE COUNT          727 a          692 c          672 g          708 t
ORIGIN      
        1 taaaaatctt gcggctctct gaactcattt tcatgagtga atttggcgga acggacggga
       61 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
      121 gcgttgtgtt ccgttgggaa cgaggcgaat attacggatt gcctcaccct tcgtcaacgg
      181 tttttctcat cttttgaatc gtttgctgca aaaatcgccc aagtcgctat ttttagcgcc
      241 ttttacaggt atttatgccc gccagaggca gcttccgcct ttcttctgca ccagatcgag
      301 acgggcttca tgtgctgcaa gctcttcatc tgtcgcaaaa acaacgcgta acttacttgc
      361 ctgacgcaca atgcgctgaa ttgttgtttc accttgttgc tgttgtgtct ctccttccat
      421 cgcaaaagcc atcgacgttt gcccaccagt catcgccaga taaacttccg caaggatctg
      481 ggcatcgagt aatgccccgt gcagcgttcg tttactgtta tctatttcgt agcgggcaca
      541 taacgcatcg aggctgttgc gcttaccggg aaacattttc ctcgccaccg caaggctatc
      601 ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg aaaactcgta
      661 gtccataaag ccgatatcga acgctgcgtt atggatcacc agctccgcgc cgcggatata
      721 gtccatgaac tcatcggcta cctcggcaaa cgtgggctta tcgagcaaaa attcatcggc
      781 aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga gataaacatg
      841 gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct caatgatctt
      901 gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat cgagaacgat
      961 ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttatcg ttttacagtt
     1021 cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt tcaccgatgg
     1081 ttcgtgtctg ggtaatcctg gacctggggg ttacggcgct attttacgct atcgcggacg
     1141 cgagaaaacc tttagcgctg gctacacccg caccaccaac aaccgtatgg agttgatggc
     1201 cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga gtaccgacag
     1261 ccagtatgtc cgccagggta tcacccagtg gatccataac tggaaaaaac gtggttggaa
     1321 aacagcagac aaaaaaccag taaaaaatgt cgatctctgg caacgtcttg atgctgctct
     1381 ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc cggaaaacga
     1441 acgctgtgat gaactggctc gtgctgcggc aatgaatccc acactggaag atacaggcta
     1501 tcaagtcgaa gtttaagcct gtggtttacg acattgccgg gtggcaccaa ccgcctggcg
     1561 aattcgcggt ttgttcttgc tctgtttcat cggatttagc gttaaaggaa tagtccgttt
     1621 ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgcgcattca atagttttcc
     1681 tccgtgtttg ttccacggga gaacgtggaa acggctggcg tgtagcactt caaaattcaa
     1741 caaagagagc cagtccagct gccgcatcag agtgaacatc cggctgttat agggcgatgt
     1801 tttgcgcaat accggcacaa gtttgcgtaa tcccattaaa ctgatgggat tgaagccact
     1861 aatcaccagc cagccatcat caatcagtac ccgatcggct tcacgcaata aacgatgcgg
     1921 atcagtgcac cacggcaatg tatgtgccag tagacaaaca tcaacggatt tatcggcaaa
     1981 aggaagatga agtgggtccg cctgcacctg gacgggcatt ccttgcgcag aaacattcac
     2041 ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc caatcttaag
     2101 cagatgaaaa ccatacattt tagtgaacca cgggttgagc tgacgctcca gcgccttgcg
     2161 ataaagctct ccccagggca aatcgcccca gcaatcagga gccacgacag tttgagggac
     2221 tcttgccggt ttcatcacaa ccttccgttt cacactgaga ggtaatctat gaatcttaac
     2281 agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc aggtcgctgt
     2341 ctgattgtcg atcccgggga cgcagagcca gtattaaacg ccatttccgc caataactgg
     2401 caaccggagg ccatatttct cacccaccat caccacgatc acgttggagg cgtaaaagaa
     2461 ctggtggaaa agtttccgca aattgtggtg tatggaccac aagagacaca agataaggga
     2521 acaacacagg tagtcaaaga tggcgaaact gccttcgttt tggggcatga atttagtgta
     2581 attgccacac cgggtcacac tttaggacat atctgttact tcagtaaacc ttatctattt
     2641 tgcggcgaca cgctgttttc tggtgggtgt ggtcggttgt ttgaagggac accatcacaa
     2701 atgtatcaat cacttaaaaa gttaagtgcg ttacctgacg atacattggt atgttgtgct
     2761 catgaatata ccttatcaaa tatgaagttt gctttgagt
//
LOCUS       DAZFRG010000388         2797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFRG010000388 DAZFRG010000000
VERSION     DAZFRG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.390"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..80
                     /locus_tag="ACHKUC_004869"
     CDS             <1..80
                     /locus_tag="ACHKUC_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO6620946.1"
                     /translation="EMENWPTYYPYQLSSEEVVEEMMSH"
     gene            928..2148
                     /locus_tag="ACHKUC_004870"
     CDS             928..2148
                     /locus_tag="ACHKUC_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6620947.1"
                     /translation="MPKKAKELSGLVVSRLKSEGMYAVGGVDGLYLRIRNQSRAWVLC
                     VAMGTRINNLGRTVPRRLNMGLGPYPEVSLAEARDKARELRKQIRNGINPLQEKHEQK
                     ARQEILARKKKTFAECCEEVLEVKDSEMKNKKHLAQWRSTLETYAYPFIGKKAVSEIT
                     KVDLLAILEPIWLTKNETASRLRGRIETVIDYAKAKEYFEGDNPAAWKGMLKPLLPQP
                     SKVQITKHHAALPYNQLGSFMKELRERSGVSPRALEFAILTAARSGEIRGAEWSEIDL
                     EGKTWTIPASRMKAAKEHRVPLSDAAVALLKALPRFKGINFVFPATRKGQLSDTALLA
                     VLKRMGYTDLTQHGFRSTFRDWAGETTNYPREVIEHALAHQLANKAEAAYQRGTLWPK
                     RVALMDDWAGYCVS"
     gene            2293..2739
                     /locus_tag="ACHKUC_004871"
     CDS             2293..2739
                     /locus_tag="ACHKUC_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6620948.1"
                     /translation="MSKLDWFNNVENRPHPWEGMGLTWLTTALNVPDVKAAINFYTEV
                     MKMVVISELEGDGGDVLFARIRYRGTNFTINKEGWDSDLKSPNTSGAMPPFIFYMYVD
                     DMITLVNEMVEAGSTVLNEPAEMFWGDLKARVKDPFGFIWDIAQKL"
BASE COUNT          835 a          560 c          643 g          759 t
ORIGIN      
        1 atgaaatgga aaactggcca acgtattatc cctatcagtt gagtagcgaa gaagtggtcg
       61 aggagatgat gtctcattga tttttttgcg ctgacttcag ttatttaaca gaacagtgcc
      121 tggtagtaac aacaataatt tgccaagaca aataagtaag acaaactcag acacgctcat
      181 acttgagtcg tgtctttttt attatgtctg tagaaaaaaa ctgcgaaaaa ggcgaacaat
      241 aaatacacaa ctcagccata cagactggca gcgcaaatgt ttccaggagc aagtcatctg
      301 tcatggtaaa gctaaccaga aatataggtt aattttggtg tttaaacctt ttttacttgt
      361 tagtatgata caaaggcttt caaaaaagct gggaaattca acaactcttg gaaattatgg
      421 actttgtccc gcggctttaa tctgacggcc gcgttccttt tttggctcaa ttattgaccc
      481 cttctctacg caacttcagt tcccgccagc aactttgcgg cagtttcata ggatcaatgt
      541 ctaaaagaat aatggtgacc gataagaaaa cgactgaata actgtagatt ttcgctcgaa
      601 accttcctgt cagacccata gcgaatcaag tgctgaatgt cacagtatcg aacagaaaac
      661 agtgacgatc taacccttca agaatattct acgattgttc tgtttaggaa aagcaaggcg
      721 ggaagtcggg agataagtca ttgataaagt ggcggagaga gggtctaaat ttttagtgtt
      781 cgataatgcc caccaaagtg ctatttttcc tttaaaaaca aaatattgtc attatttttt
      841 tgttcggtag tgtattgcta ctgtgcaaac aatcagctat tttatgcagt accgtatgta
      901 tggcgggttg tatgacagga ttttgctatg ccaaagaagg ccaaggaact ctcaggactc
      961 gtcgtttcac gattaaaatc cgaaggcatg tatgccgttg gtggtgtgga tggtttatac
     1021 cttcgcatca gaaatcagtc tcgcgcatgg gttctttgcg tggctatggg aacccgaatc
     1081 aacaatctcg gtagaacagt tccaagacgt ttgaatatgg ggcttggccc ttacccggaa
     1141 gtctccttag ccgaagcgcg tgacaaagcg cgtgagctac gtaaacaaat ccgtaatggt
     1201 attaatcccc ttcaagaaaa acacgagcaa aaagcccggc aggaaatact ggcccggaag
     1261 aaaaagacct ttgccgaatg ttgtgaagaa gtgctggaag tcaaagacag tgagatgaag
     1321 aacaaaaaac atctcgcgca atggcgttcc acactggaaa cctatgccta ccctttcatt
     1381 ggcaaaaagg ccgttagtga aatcaccaaa gtggatcttc tggcaatact ggaacctatc
     1441 tggttaacca agaatgaaac tgccagtcgc ctacgtggac gtattgaaac agtgatcgat
     1501 tacgccaagg ccaaagaata ctttgaaggt gataatcctg cagcatggaa aggtatgctg
     1561 aaacctctgt tgcctcaacc aagtaaggtt cagatcacca aacaccatgc ggcccttccc
     1621 tataaccagc tcggctcctt tatgaaagaa ctacgtgaac gaagcggcgt ttctcctcgt
     1681 gcgcttgagt ttgccatact gaccgcagca cgctcaggtg aaattcgtgg tgccgagtgg
     1741 tcagagatcg atcttgaagg taaaacatgg accatccccg ccagtcgaat gaaagcagcg
     1801 aaggagcatc gagttccgtt gtctgatgct gctgttgccc tgttaaaggc tttaccacgc
     1861 tttaaaggta tcaattttgt attccctgcc actcggaagg gacaactctc ggatactgct
     1921 ttactggcag tcctaaagcg aatgggatat accgacttaa cgcagcatgg gtttagatcg
     1981 acattccgtg attgggctgg tgaaacgacc aactacccgc gtgaggtaat tgaacatgcg
     2041 ttagctcatc agttggcaaa taaggcagaa gctgcgtatc aacgtgggac gttatggcct
     2101 aagcgggtgg cgttaatgga tgattgggcg gggtattgtg tttcttagaa gttctaatta
     2161 gctacaaata gtccccgccc tttatgaact gacaccataa attcttaatg attgtataag
     2221 atttatcaat ttagagtaat atacgacaga aacgtttcac atgaatatgc tgtttttaag
     2281 gagaaaaatg aaatgagtaa actggactgg ttcaataacg tagaaaatcg cccacatcct
     2341 tgggaaggta tggggctgac atggttgaca acagcactaa atgtccctga tgtaaaagca
     2401 gctattaatt tttacactga agtaatgaag atggtggtaa tttctgaact tgagggtgat
     2461 ggtggtgatg tattatttgc tcgtatccgt taccgtggca caaacttcac cataaataag
     2521 gaaggatggg attccgacct gaaaagccca aatacttcgg gagctatgcc tccctttatt
     2581 ttttatatgt atgtagatga tatgatcact ctggtaaatg aaatggttga agctggctcg
     2641 actgttctaa atgaacctgc tgaaatgttc tggggtgatt taaaagcccg tgtgaaagac
     2701 ccatttggtt ttatctggga tatagcacag aaactgtaaa gaaatcgtca gatcatcgtt
     2761 caactcctct tttcggttaa agtaggctgt tgaaatc
//
LOCUS       DAZFRG010000389         2782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFRG010000389 DAZFRG010000000
VERSION     DAZFRG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.391"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..671
                     /gene="comR"
                     /locus_tag="ACHKUC_004872"
     CDS             39..671
                     /gene="comR"
                     /locus_tag="ACHKUC_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO6620949.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVASALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(733..1272)
                     /gene="ycfJ"
                     /locus_tag="ACHKUC_004873"
     CDS             complement(733..1272)
                     /gene="ycfJ"
                     /locus_tag="ACHKUC_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6620950.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
     gene            complement(1499..>2782)
                     /gene="ndh"
                     /locus_tag="ACHKUC_004874"
     CDS             complement(1499..>2782)
                     /gene="ndh"
                     /locus_tag="ACHKUC_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO6620951.1"
                     /translation="IVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLL
                     HEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVP
                     ERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGA
                     NGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALP
                     PRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLK
                     DIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCA
                     MNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYIS
                     LYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          659 a          775 c          688 g          660 t
ORIGIN      
        1 ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat
       61 gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg
      121 ccatgaaatt gttctggcag cacggttatg aagcgacttc tcttgcggac ctcgttgaag
      181 cgaccggagc taaagcgccc acgctatacg cggaatttac caacaaagag gggttattcc
      241 gcgccgtact cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt
      301 gtgaagagaa aagcgtggcg tctgcgctgg ctgactattt tgctgccatc gccaactgct
      361 ttaccagcaa agacaccccg gcgggctgct tcatgatcaa caactgcacc accctctccc
      421 cggattctgg agatatcgcc aatacgctga aatcacgcca tgcgatgcaa gagcgcactt
      481 tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga
      541 cgcatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag
      601 gtgcatcgct ggaaaaactg atgcagattg cccgaacgac tttgcgttta tggccggaac
      661 tgctgaaata agtcgcaaaa aaagcccctc agcttgagcg aatgaggggc taaattgcag
      721 agtacaacct tgttatgctt tgttattcaa aatcagctgc ccgttgctat ctagcgggat
      781 ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt
      841 cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt
      901 agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttacctg catatccacc
      961 ccccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc
     1021 aatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac
     1081 cggtcgacga tgggtcactg taacgttgcg acactcctga cgcggtgttt taaccgtttc
     1141 cttgattggg gttgcagaaa caacctgagc gtattgcggg ccacgttcaa acacgttcag
     1201 actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac ccgccaacat
     1261 tgacttattc acgggaatac ctccttcttt gctgttatgc caatcatgca agcgaaggag
     1321 aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata cttagggatt
     1381 cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct
     1441 cgtaccattt gtccagggcg gataaggcgt ttacgccgca tccgccagta caagccgatt
     1501 aatgcaactt caaacgtgga cggataacac ggttaatact ccccaccagc atcattaatc
     1561 cggttttaaa gtaaccatgc agcgcaatct ggtgcattcg gtacagcgag atgtatacaa
     1621 agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac
     1681 cgacggtgga aaagttcgac agcgatacca gcgaaccgtg atctttatac tgataatttt
     1741 tcagcggctt accgttcatc tgcgccagaa tgttgttcat tgcacaggtt gccatctgat
     1801 gtgcggcctg agcgcgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt
     1861 cgccaatagc gtatatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct
     1921 ggttgatacg gttggtttca agaccaccga tatctttcag gaagtctggc gctttgatcc
     1981 cggctgccca caccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc
     2041 cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgcctagt ttcgttaact
     2101 cactatgggc cgcagcagag atacgtggcg gcaatgcagg caaaatacgt tcgcccgctt
     2161 ctaccagcgt tacgttcagg gcttcgttgg tcagaccttt ataaccgtaa ctgtgcagtt
     2221 gcttgaccgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa
     2281 tcgcaatgtt tactttgcca ttcgcgccca ggttggcgga gtatttcagg aacagattca
     2341 gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt
     2401 tgacacctgg tgtattgaaa tcgttagagg tgctacccag cgccattacc agggtgtcat
     2461 aggcgatttt acgttccgga accagcagtt cacctttctc gtcgcgcagt tctgcaatag
     2521 tgattgtttt cgcttcacga tcaatatcaa ttacggaacc cagctggaac tggaaaccat
     2581 gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg
     2641 ccacttcgtg cagcagcggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt
     2701 tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag
     2761 caccgccgcc gacaatcaca at
//
LOCUS       DAZFRG010000390         2746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFRG010000390 DAZFRG010000000
VERSION     DAZFRG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.392"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1239
                     /locus_tag="ACHKUC_004875"
     CDS             <1..1239
                     /locus_tag="ACHKUC_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO6620952.1"
                     /translation="VLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKEDLI
                     KLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNNGGI
                     VMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLDDST
                     KLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVETGPI
                     GGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKFNGK
                     IMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEITFS
                     GKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKL
                     MDERLFIDAAMGFVLPEAAH"
     gene            1253..2404
                     /gene="ydiO"
                     /locus_tag="ACHKUC_004876"
     CDS             1253..2404
                     /gene="ydiO"
                     /locus_tag="ACHKUC_004876"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO6620953.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2447..>2746)
                     /locus_tag="ACHKUC_004877"
     CDS             complement(2447..>2746)
                     /locus_tag="ACHKUC_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6620954.1"
                     /translation="KFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQ
                     AEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          736 a          633 c          708 g          669 t
ORIGIN      
        1 gtacttacac aaaccttacg cgccgccgca gcccaccagc ctggtattat tagtgatatt
       61 ggcatcggaa catttgtcga tccacgccag caaggcggca aactgaatga agtcactaaa
      121 gaagacctga ttaaactggt cgagtttgat aacaaagaat atctctatta caaagcgata
      181 gcgccagata tcgccttcat tcgcgctacc acctgcgaca gcgaaggcta cgccactttt
      241 gaagatgagg tgatgtatct cgacgcattg gttattgctc aggcggtgca caataacggc
      301 ggtattgtga tgatgcaggt gcagaaaatg gttaagaaag ccacgctgca tcctaaatct
      361 gtccgtattc cgggttatct ggtggatatt gtggtggtcg atccggatca aacccaactg
      421 tatggcggtg cgccggttaa ccgctttatt tctggtgact tcactcttga tgacagcacc
      481 aaacttagcc tgcccctaaa ccaacgtaaa ttagttgcac ggcgtgcatt attcgaaatg
      541 cgcaaaggcg cagtggggaa tgtcggcgtc ggtattgctg acggcattgg cctggtcgcc
      601 cgggaagaag gttgtgctga tgactttatt ctgacggtag aaacaggtcc gattggcggt
      661 attacttcac aggggatcgc ctttggcgcg aacgtgaata cccgcgccat tctggatatg
      721 acgtcccagt ttgattttta tcacggtggt ggtctggatg tttgttattt gagttttgct
      781 gaagtcgacc agcacggtaa cgtcggcgtg cataaattca atggtaaaat catgggcacc
      841 ggtggattta ttgatatcag tgccacttcc aagaaaatcg ttttctgcgg cacattaact
      901 gcgggcagtt taaaaacaga aattaccgac ggcaaattaa atatcgtcca ggaaggacgg
      961 gtgaagaaat ttattcggga actaccggaa ataactttca gcggaaaaat cgctctcgag
     1021 cgagggctgg atgttcgtta tatcactgag cgcgccgtat tcacgttaaa agaagacggc
     1081 ctgcatttaa tcgaaatcgc tcctggcgtc gatttacaaa aagatattct cgacaaaatg
     1141 gatttcaccc cagtgatttc gccagaactc aaactgatgg acgaaagatt atttatcgat
     1201 gcggcgatgg gttttgtcct gcctgaagcg gctcattaac aggagtataa tgatggattt
     1261 ttctttaact gaagaacaag aactgctgct ggccagtatt cgcgaactga ttacgactaa
     1321 ctttccggaa gagtatttcc gcacctgcga tcaaaacggg acatacccgc gtgagtttat
     1381 gcgggcactg gcggataacg gtatttccat gcttggcgtg ccggaagaat ttggtggtat
     1441 ccctgcggat tacgtcaccc aaatgctggc gctgatggaa gtgtcaaaat gcggtgctcc
     1501 ggcatttttg attaccaacg gtcaatgtat tcacagtatg cgccgtttcg gttctgcaga
     1561 gcagctacgt aaaacggcag agagcaccct ggaaacgggt gatcccgcct atgccctggc
     1621 attgacagag ccaggcgcag gctcagacaa caacagtgcc actaccactt acacgcgtaa
     1681 aaacggcaag gtttacatca acggacagaa aacctttatt accggtgcga aagagtaccc
     1741 atatatgctg gtgttggcgc gcgatccgca accgaaagat ccgaaaaagg cctttactct
     1801 gtggtgggtc gactccagta aacccggcat taaaatcaat ccactgcata aaatcggctg
     1861 gcatatgctc agcacctgcg aagtctatct cgacaacgtg gaagttgaag agagcgacat
     1921 ggtgggcgaa gaaggaatgg gtttcctcaa tgtgatgtac aactttgaga tggagcgcct
     1981 gatcaacgcc gcgcgcagca ccggctttgc cgaatgcgca ttcgaagatg ccgcccgcta
     2041 tgccaaccag cgtatcgctt ttggtaagcc cattggtcat aaccagatga tccaggaaaa
     2101 actggcgctg atggcgatta agatcgacaa catgcgcaac atggtgttga aagtggcatg
     2161 gcaagccgat cagcatcagt cactgcgcac cagcgcggcg ctggcaaaac tgtactgcgc
     2221 acgtaccgca atggaagtca ttgatgatgc gattcaaatc atgggcggtc tgggctatac
     2281 cgatgaggcg cgcgtctccc gcttctggcg tgatgtccgt tgtgaacgta tcggcggcgg
     2341 tacagacgaa attatgattt acgtagcagg tcggcagatc ctgaaagatt atcagaacaa
     2401 ataatctgca ggcggcgcag cttcttaaca aactgcgccg ccagatttat ccggcaagac
     2461 ttactggttg aggaaattca gataggattt cttgttccgc aaaacagttt ttaaattgcc
     2521 tgcggtaatc gctgggcgaa cagccgacat ggcgcaaaaa cagtttggca aagtgatcga
     2581 cattttcata acccacacgc caggagatct ctgcctgtga taattcagta ttagtgagtg
     2641 accatttcgc ttccgtcata cgccgttgaa taacatagtt aataggggaa atacgatact
     2701 ctttggtaaa ttcatggcag atataactga cgctggcacg aaattt
//
LOCUS       DAZFRG010000391         2733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFRG010000391 DAZFRG010000000
VERSION     DAZFRG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.393"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..2322
                     /gene="ydeP"
                     /locus_tag="ACHKUC_004878"
     CDS             43..2322
                     /gene="ydeP"
                     /locus_tag="ACHKUC_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO6620955.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLCERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2570..>2733
                     /gene="safA"
                     /locus_tag="ACHKUC_004879"
     CDS             2570..>2733
                     /gene="safA"
                     /locus_tag="ACHKUC_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO6620956.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAINQLG
                     ISEMGRIARDL"
BASE COUNT          737 a          635 c          668 g          693 t
ORIGIN      
        1 tattacaaat cctaataatt catttccaca caggataagc agatgaagaa aaaaattgaa
       61 tcctaccagg gagctgccgg tggttggggt gctgttaaat ccgtagcgaa tgcagtacgt
      121 aagcagatgg atatacgcca ggatgttatt gccatgtttg acatgaataa accagagggc
      181 tttgactgtc cgggatgtgc atggccagat cctaagcaca gtgcgtcatt cgacatttgt
      241 gaaaacggcg caaaagcaat cgcctgggaa gtcacggata agcaggtaaa tgcctctttc
      301 tttgctgaga atacggttca atcattactt acctggggag atcacgagct tgaggctgcg
      361 ggacgtctca ctcagccttt gaaatatgat gacgtcagcg actgttacaa gccattaagc
      421 tggcaacaag ctttcgacga aattggcgct cgccttcaaa gctatagtga tcccaatcag
      481 gttgaattct atacttcggg gcgcacttcc aatgaagctg cctttcttta tcagcttttt
      541 gcccgtgaat acgggagcaa taactttccc gactgctcca acatgtgcca tgaaccgacc
      601 agtgtgggat tggcagcgag tatcggtgtg ggtaaaggga ccgtgttgct ggaagacttt
      661 gagaaatgcg atttagtcat ttgcattggg cataaccctg gtacaaacca ccctcgcatg
      721 ctgacttcgt tgcgcgcttt agtgaaacgg ggagcgaaaa tgatcgccat caatcctcta
      781 caggaacgtg gcctggagcg atttaccgca ccgcaaaacc cgtttgaaat gctgacgaac
      841 tctgagactc agttggccag tgcctactat aacgtgcgca ttggtggcga tatggcattg
      901 ctcaagggga tgatgcgcct gttaattgag cgcgatgatg ctgcaagcgc cgcaggtcgg
      961 ccctcattgc tggatgacga atttattcaa acgcataccg tcggctttga cgagctacgc
     1021 cgtgacgtgc tcaattccga gtggaaagat atcgaacgta tttctggact aagtcagaca
     1081 caaatcgccg aacttgctga tgcatatgcc gctgccgaac gaactattat ctgttacgga
     1141 atggggatca ctcagcacga acatggtacc cagaacgtac agcaactggt caatctgctg
     1201 ttgatgaaag gtaacattgg caagcctggt gcgggtatct gcccactacg aggtcactct
     1261 aatgtacagg gcgaccgaac cgtcggtatc accgagaaac cgtctgcaga gtttctggat
     1321 cgcctgtgtg agcgctatgg cttcacccca cctcatgcac ctggacatgc tgcaatcgcc
     1381 agcatgcaag ctatatgtac ggggcaagct cgagcattga tctgcatggg gggcaacttt
     1441 gcactggcaa tgccagatcg ggaagcgagc gctgtaccgt taacgcaatt agatctggcg
     1501 gtacacgtag ccactaagct taaccgctct catctgctga ccgcacggca tagctatatt
     1561 ctgccggttc tgggacgtag cgagattgac atgcaaaaaa gcggtgcgca ggcggtaacc
     1621 gttgaagatt caatgtcgat gattcatgcc tcgcgtggtg tgttaaaacc cgccggtgta
     1681 atgctgaaat cagagtgtgc agtggtcgcg ggaatcgcgc aagcagcact accccagagc
     1741 gtggtagcct gggagtatct ggtggaagat tatgatcgca ttcgcaatga cattgaagct
     1801 gtgcttccag agtttgccga ctataaccag cgcatccgtc atcctggtgg ttttcacctg
     1861 ataaacgcag cagctgaaag gcgctggatg acgtcgtcag gtaaggctaa tttcattacc
     1921 agtaaagggc tgttagaaga tccctcttca gcgtttaaca gtaagctggt catggcgaca
     1981 gtacgcagtc acgatcagta caacacgacg atttatggta tggatgatcg ctatcgaggg
     2041 gtattcggtc aacgagatgt ggtctttatg agtgctaaac aagctaaaat ttgccgtgta
     2101 aaaaacggcg aaagagttaa tcttattgca ctcacgccag acggtaaacg tagctcacgc
     2161 cgcatggata gattaaaagt ggtcatttac cctatggctg accgctcact ggtgacctat
     2221 tttccagaat cgaatcacat gctaacactt gataaccacg atccattaag tggcattcct
     2281 ggctataaaa gtattccggt tgaattagaa ccatcaaatt aattcgcctc ctcatttctt
     2341 ctgctgtcat ccgcacagca gaagaattcc tcattgacta ttatcgcgca atttgctcac
     2401 atggattaaa ttaaaccaca cactataaga tataaacttc tgcctacagc tgtaagaaac
     2461 tccgctcagt actgaagtac ccgtcctatt tcctctattc tccagcctgt tatattaagc
     2521 atactgatta acgatgctta acgtcattcg tttaataaac acatttgaaa tgcatgcgac
     2581 catagtgaaa aataaaatca cgcaaagaga caactataaa gggatcatgt ctgtaattgt
     2641 ggttgtctta ttactgaccc ttacattgat agccattttt tcggcaatta atcagctggg
     2701 tatttcagaa atgggtcgca ttgcaaggga tct
//
LOCUS       DAZFRG010000392         2731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFRG010000392 DAZFRG010000000
VERSION     DAZFRG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.394"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHKUC_004880"
     CDS             complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHKUC_004880"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO6620957.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIANGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPF"
BASE COUNT          645 a          722 c          640 g          724 t
ORIGIN      
        1 aaacggcgcg aagatttcgc taaatgcgta cttcggagcc tgttctttag tcagaccgtg
       61 gaattccgga gacaggttag tgccgcccgg caggtcgcgc gcgattgacc agataccaac
      121 cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag agagatagaa
      181 cacctcaccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca tggcgtcaat
      241 ttctgcatca ctcttattgc catagaggcc tttcagctgc gcatgcaggt tggcaatcgc
      301 agacgttgca cacttaccgt ggatgttctg gccttcggtg tttgcagact gacaaatagc
      361 gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt tcgctttggc
      421 atcgctcagg acattcatcc cttccggcgt caggcctgtc ggcagaattg gcaaggtata
      481 ccagatagca atatctttgc cttctgactt ccatttatcc tgcactttct tcacagcaag
      541 gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat cgaagtccag
      601 gactctgagg ttcaggttat caacgatgtc ataataatgc tgcattagat cgtcaacgtt
      661 cttacaggaa gctgccagcg gggcattatt agcaccaccg atagacagca tcacatcacc
      721 gccagcctca cgcagagctt tgattttgct gtactgagcg taattctgca taccgtaagc
      781 ggtaccccat gtcggtagac aggtgttcgc atctttactc accacaaacg ccagcgtgaa
      841 gtggttgacg ttatgattct tagccagcgc agccaaatcc gggatcgtat tcagcgtaaa
      901 gtcgacatac ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac ctacgcgctc
      961 tttggttccg gtccagtcaa caaaaacacg ccacgggttg ctgtcagaag gagaaacttt
     1021 ctgcacttta ctctgagaaa tgtagttctc accgttaaag cgaatcagcg tatcactggc
     1081 ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttgc tatagctctg
     1141 agtcttacct aacggcttcc acgggcgact attgctaccg gtggcgtttt ggttcgcaac
     1201 cagagcaggg ttgtcacttt gcgtccagaa cagcgcttca taagcctgcc catcaacaac
     1261 aacgcgatca cctttcacgt acacggtgga agcggaccat gctttcgccg gttcgtattt
     1321 cttccacggg ttatcgccgc cggggacaaa gccccatgcg tcttgatcga cagaggtttt
     1381 ataatgtgca tcgttataat ctacgatgct gttcgcgctg tacgctttgc tggcctggaa
     1441 accattcgca ataatgacgc cgccattatc aatttcacag gtagtcgggt taccgtactg
     1501 actcatttcc gcagcggtag ctgtacgcac gaggcgccat gggttattgg agttttctac
     1561 actggtgcca cgcgggcaat cttcagaacc aacccaccag gcattctgat aaacattacc
     1621 attgaagata acatggtaaa tttcgctgcc ttcctggcca ctccacgcct gcagtgagta
     1681 atcgttactg ctatccgctg gtgttgatgg ctcgttgtta tccggagttg gagtaactgg
     1741 agtttccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat tattatccgg
     1801 cttaacggaa caggagcctg ggttaccata ctgagtgatt tctgttgccg ttgccgcgcg
     1861 aacataacgc catgggttgc tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
     1921 gcccacccac caggcattct tataaatgcc gccgttgtag ataacgtacc atgtagaaga
     1981 accttcctga cctttccagg ctacgacagc atagttgtcg tttgacttat cagctggggc
     2041 tggctgaaca tcaggagccg gtttaacgtc cggtgccggt ttcacatccg gtgcaggctg
     2101 tggtgctgaa gtacaagact gtggattact ggtttctgag atttccgttg ccgtagcggc
     2161 acgaacataa cgccatgggt tgctggcatc attgcctttc gcatcacccg ggcagttaga
     2221 agaggctacc caccaggcat ttttatatac cgcaccatta aagacgacat accaggtttg
     2281 accgccctgc tgtttattcc aggcaactac agaagaatta gacggaacag tgccctgtgc
     2341 tggtgtagcc gaaccgccat tagcaggcgt attagatgct ggcgtatttg aatttgaaga
     2401 agatgagctg ccgctctttt cgcaggaaag tgtattgcca aactgactaa tttcagcagc
     2461 tgttgcggta cgctttaaac gccacgggtt agttgcatca tttgctttcg cttttcccgg
     2521 gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga aaataacctg
     2581 atatttatta ccaccttgtt ggttattcca tgcttccatt gccaatgctg gcagagcgga
     2641 acacaccagc cccataccaa tcatagattt agtaaatata tttaatttca ttataatccc
     2701 ttgtaacgta aaaactgcaa aacaaaagtc c
//
LOCUS       DAZFRG010000393         2727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFRG010000393 DAZFRG010000000
VERSION     DAZFRG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.395"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..193)
                     /locus_tag="ACHKUC_004881"
                     /pseudo
     CDS             complement(1..193)
                     /locus_tag="ACHKUC_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
     gene            290..583
                     /locus_tag="ACHKUC_004882"
     CDS             290..583
                     /locus_tag="ACHKUC_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO6620958.1"
                     /translation="MWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGGLWGISW
                     GWAMWFMYWGPSGMVAGEAIIISITSGFLFGLLMASFHWWRRKVNRLPPWDDV"
     gene            complement(661..962)
                     /locus_tag="ACHKUC_004883"
                     /pseudo
     CDS             complement(661..962)
                     /locus_tag="ACHKUC_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1143..2177
                     /locus_tag="ACHKUC_004884"
     CDS             1143..2177
                     /locus_tag="ACHKUC_004884"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFO6620959.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAREQHIVYMTSLTNS
                     ISTLLTDGNPHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     KRQISVAHQVINNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTE
                     QLLSTLAAQL"
BASE COUNT          773 a          573 c          614 g          767 t
ORIGIN      
        1 gaacagcatc attcgggacc cagagaataa gataatagtt ttcaatgttc tcaggaataa
       61 cctgcagaag gctgtctccg gtattgacca tttgccctac agtgacactc agggagtcaa
      121 ctttcccgtc agacaacgcc cggataatta tttcgccttc cacatcagtg ttaaccagtt
      181 ctttctgcaa ttcgagtagt tgtgtcacgt ttacatcatg gaacggaggt gctcctgtga
      241 cttttgagca aaaaaagccc gggccattgc cctgatggac agcaaaaaga tgtggcggag
      301 taattatgct cctcctctgc tgcgtattct ctggcggctg ggtatccggc ttccgccatt
      361 gccctttatg ccgttctggc aggttacgct gctaatgggg ggcttatggg gtatttcctg
      421 gggatgggca atgtggttta tgtactgggg gccgtcagga atggtagcag gtgaggccat
      481 tattataagt atcacgagtg gttttttgtt cggactgctc atggcctctt tccactggtg
      541 gcgcaggaaa gttaaccggc taccaccatg ggatgatgtg taaaccgtgc cggcatgttt
      601 ttacggggtg atgctttcat taacctgtac acgaacttct attcctgcat cattacaggc
      661 ctacagccac tacgccactt ccagcggatc cccctccctg cgtacctccc cgccttcttt
      721 attctataac tgcagacagg tgctgccgtc gaggcgcacc acaaaatccc cacggcaggc
      781 ctgatatgtc atgccgttca gctccaccgg cagatcagtg gcagaagtgc catctgcggg
      841 cagacctgat gccgggacca gcggcagtac gacgacttct gcagtacgct gttctgccag
      901 cagagaggct gtgccacggc ccggtcagct tttgtgagtt ccacagctag ccgcagctca
      961 gggattaaga ttcaggtaat ttacggaaaa caagtgattt ccgtacggga gagaaggcct
     1021 aacgtatatt atctcagaaa tgactatgaa tccccatata ttgccaatta cacagtaaat
     1081 gattatcatt atcatttact gtgtgcaact gatggtctga aatagagctt ttaggagaca
     1141 agatgcagtc agctagtaaa ttaatctacc gcgggaagtt gcttggcagt ttacccactg
     1201 tgggcgagat tcataaagaa atcgcagtat cggaagaaac cgtaacatgg atctctctgc
     1261 aacgagatat tatagcgaat attttgctgg gaaaagatcc tcgcttactt gtgattgttg
     1321 gtccatgctc aatccatgac gttcaggccg cagttgatta tgcgaagcga ttgttcgtat
     1381 tacagaataa gtacttatcg aaaatgtaca ttgtaatgcg cacttatttt gagaaacctc
     1441 gaacccgtaa aggctggaag ggaataatgc acgatcctga tctaaatgga agttatgatg
     1501 ttgagaaagg gatacgatat gctcgccagt gtttgtcttc aatcacaacg atgcgtgtcg
     1561 caaccgctac agaattcctc gatcctttcc ttactcccta tattgctgat ttaatatgtt
     1621 ggggtgctat tggtgccaga accactgaat cacaaacgca tcgtcaactt gcgtctggtt
     1681 tacattgtcc agttggattt aaaaacagta ctgatggaaa tattaaccta gctattgacg
     1741 ctattattgc agcccgtgaa caacatattg tatatatgac atctttaaca aatagtatat
     1801 ctacactatt aaccgatggc aatccacatg gacatctaat attacgtgga ggtagagaac
     1861 ctaactatgg tttgtctgat ataactaagg ctgtgaaatt aatgcatgat gagggcatta
     1921 accatcgtct gataattgat tgtagccatg gtaatagcgg taaagtggct aaacggcaaa
     1981 tttccgttgc acatcaagtt ataaataatc ggaaaaaaat accaggatat gtagctggca
     2041 ttatggtgga aagcttttta caagatggta aacaatccga tagttttccg ctagagtatg
     2101 gtcagtctgt tactgatgaa tgcatatcat ggcaacagac agaacaatta ttatcaactc
     2161 tggcagcaca gctttaactt caactattgc acatatattg cttttaaagg ggggggatga
     2221 aattacttag tttaagacac tcaattagtg tcatcagaga agcagatatt atatctctac
     2281 agtttatgat tatataaagc gctatcgtag ctgcgtattt aatggtctaa aaatataaaa
     2341 caattggtta ctatctatgg caagctcctg gattatgtgt gtgaaccagg agtttctcag
     2401 gaatttcctc acaatgtggc actatcactg tcaccttttt ctccttggaa acctccatgg
     2461 atgcctcaaa gtttcggtca gtttttagat aggtagcatt cctcattaat aatgaggaca
     2521 ccgagggtag aatcttaact ccgtgttaat agaataagaa taaaaaacaa tcatcagaac
     2581 tgagtaggga aagcgaatca tgatgtatga agtttcacac acaatgtgaa tcaaaaacgc
     2641 tcgtagataa ttcgataatt ctgcctgaaa tgcaggtcag aaacaatgtg aacattatcc
     2701 gtgagaaatt gtgacaaaat tcccgat
//
LOCUS       DAZFRG010000394         2689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFRG010000394 DAZFRG010000000
VERSION     DAZFRG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.396"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..261)
                     /locus_tag="ACHKUC_004885"
     CDS             complement(<1..261)
                     /locus_tag="ACHKUC_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620960.1"
                     /translation="MAVSIAEYLGQRTDVDQQIVPVAKGTQIQCPFMDRTCDKASKVK
                     NPTPPVCSVRKPDGTVWIVCEHRLCSTRQKKTVIVNGRKKQIE"
     gene            complement(963..1256)
                     /locus_tag="ACHKUC_004886"
                     /pseudo
     CDS             complement(963..1256)
                     /locus_tag="ACHKUC_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141174869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..1635)
                     /locus_tag="ACHKUC_004887"
                     /pseudo
     CDS             complement(1450..1635)
                     /locus_tag="ACHKUC_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1858..2604
                     /gene="tia"
                     /locus_tag="ACHKUC_004888"
     CDS             1858..2604
                     /gene="tia"
                     /locus_tag="ACHKUC_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion outer membrane protein Tia"
                     /protein_id="HFO6620961.1"
                     /translation="MKKVIVVSALAMAGVFSAQALADRGKTGFYVTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSHY
                     TLDTWHSPMGDGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDISALHYSGYDNNFAWSIGAGVRYDITPDIALDLSYRYLDAGKSS
                     LSYKDAEGDKYKSEADVKSHDIMFGVTYNF"
BASE COUNT          676 a          605 c          630 g          778 t
ORIGIN      
        1 ttcaatttgt tttttacgac catttacaat gactgtcttt ttctggcgag tagaacatag
       61 ccgatgttcg caaactatcc aaacagttcc atctggtttg cggacactgc atacaggagg
      121 tgtaggattt ttgaccttac ttgctttgtc acaggttcga tccataaaag ggcattgtat
      181 ttgcgtacct tttgcaacgg gaacaatctg ttggtctaca tctgttcttt gacctaaata
      241 ttccgcgata ctcactgcca ttgctcattc ctcagtcaag aatcttatac gatatgatca
      301 tagtgagtgg taatttatgt caattaattc atagaacttc tatatcttag gtgtaagttt
      361 taaatgaaaa tgaaggaagt ccgagtcttg acgctgctca tctctcgatg cactcattat
      421 ttcccaagat agtgaagcca atctgacttg ctccccgttg attaatacac cgtggtgtta
      481 gccatgcctt catcagcaac gtaagggcat tcctatgaag aagtgattta cagttttgtc
      541 cctgtggacc tgccgctgtc agaataatac tccccctgat gagcgtatca gagatggtat
      601 gtcggcattt tgcgggttgc tcccgtcagg atgcgcgacc tgttaccctg aaaagttgag
      661 ctgaccactc agttctggcg agccgcttac gacactggga ctacgcgcgg ataccaaata
      721 gctgctgaat caggcaataa cagtggcgtt ccgcagtagc ttggctggca gcgaatgtat
      781 ggagatcggg aaagaggagt taaattgcgg tttactggca cagatgcaca atgtcaacgc
      841 cacgatgttt gaccgttatg gggatgcgga cgatttcctg cacagcgttg tcctcgcggc
      901 ttttacccgg tcaaaacagc gttacggtgc accacgcctg acggatgaac tgcgtgctca
      961 gtgttaccac tggttgagca cagttccgcg taatgtagtt cccagaagcc atcgcccttc
     1021 agacgccaga acggcatgtc cgggcgctgg tcacggcgct gcggcccata acgttccagg
     1081 agatccaaca ggggctcgta gatttcagaa gcatagttaa acaggcgacc atgaccctgc
     1141 cgatagtgtg agagaacgta cagcagcaat aatggcttat gtggcgcacg ttgttcacct
     1201 ttgcgccaga tggtgatatt tgatatcgct tgttttagga ttttgtcaga ggtcattgcg
     1261 ttgaagaatt catgagcata tgtttgcgat gatgctcgca actatatgta tattcaaggc
     1321 attattgcag acatcctttt tgcctgacgg ctctgtacct ggcagatgaa atacagcgac
     1381 caaccagtcc taagcggtct ggttggtctt ttaaatttaa acatcagcca aggagtaacg
     1441 gcattcaact cactcgccat taccccgatt gatcttcgca tagtcaaacg gcgtaatcgc
     1501 tctttcccga ttggcatcca gaaaatcgac ccaccactgc aacatcagct tacgcttatc
     1561 cagatattcc gctttatgga ttatacacgg cctgcactga gttacgtcca tgtggctcat
     1621 ctgacgttca gccgctttat ctcgtgctgt tctggtctca atacttccat gccgttgcaa
     1681 ttacctctta aaccattcct taccattttc atgtcttcga taagaaatct tcatcatgca
     1741 tgatcgattt tgtcgatcga ttcgatcgaa ttttctcttt ttaccctgtc ttttgcatgg
     1801 ttttaaaata agcaaccttc tttatcgtgc gggtaagaac caacggagat gattgaaatg
     1861 aaaaaggtta ttgttgtttc agcgcttgct atggcgggcg tattttcagc ccaggctctg
     1921 gctgataggg gtaaaacagg cttttatgtg accggtaaag ctggtgcttc ggttgtgact
     1981 cagactgacc agcgcttccg tcaggacttt ggggatgatg tttataagta taagggcggt
     2041 gataaaaacg atactgtatt tggtgcaggc cttgcggtgg gctatgattt ttatcaacat
     2101 tacaatgttc ctgtgcgcac ggaagtggaa ttctatggcc gcggagctgc agactcccat
     2161 tatacactgg atacatggca ttctccgatg ggggatggtg gtcgggaaga cacacagaac
     2221 aggctcagtg tgaataccct gatggtgaat acgtattatg acttcagaaa cagcagtgca
     2281 ttcactccat gggtgtctgt tggactgggt tatgcacgga tacatcataa ggcaacatat
     2341 actgatacct cctggaataa atctggtgag gtgagcgata tttctgcgtt gcattactcg
     2401 ggatatgata acaatttcgc atggagcatt ggggccggtg ttcgctatga cataaccccg
     2461 gatatcgctc ttgacctcag ctatcgctat ctggatgctg gtaaatcctc cctgtcttac
     2521 aaggatgcag aaggggataa atataaatca gaggctgacg ttaaaagtca cgacatcatg
     2581 tttggtgtga cttataactt ctgacgacac tgctcctgag cgataattac acatattcag
     2641 taattaagat atttatatat cttctgcaac taaacagaaa taccctgca
//
LOCUS       DAZFRG010000395         2653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFRG010000395 DAZFRG010000000
VERSION     DAZFRG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.397"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(159..1339)
                     /locus_tag="ACHKUC_004889"
                     /pseudo
     CDS             complement(159..1339)
                     /locus_tag="ACHKUC_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            1926..2155
                     /locus_tag="ACHKUC_004890"
                     /pseudo
     CDS             1926..2155
                     /locus_tag="ACHKUC_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          650 a          655 c          666 g          682 t
ORIGIN      
        1 aacctcataa aaaatcctgt actcacaggg tgatgcggga taactgacgt aaggagtgct
       61 gcagggattt gatggtccct tgtgctgata tgaatacgga tattttatac agaatacttt
      121 tcagtacaag cgactgtatt acagtctgcc atatatgatt acctgtgatc aggaagaata
      181 agtggctgag gtttcaaact gtctgccggt atattcctct ctcccttaaa aaccataaca
      241 ggttagttat cttcgtctgt cagcggattg aatggcggta tattttcagt acaaataccg
      301 gtcatccact gaaaaatacc tgcgaaatga cgggcactga tttttctact gacgggctga
      361 tgagacgtta tgtcactggc ggtaataatc agtggcacac tgtagcctcc ctgcacatga
      421 ccataatgat gaacaggatt ggcactgtcg ctgaccgaca gcccaaggcc agaaaagtaa
      481 agcatggcga aatgacgtga gtaccggcga aggataccat caagctggcc aagaaagtta
      541 tccagtttac tgatgctggc gaggtaacag gcaatttttc ggggatactg ctccaggtaa
      601 ttattcggct aggagttaag gctgtcacac ggatttggat gagaacccat catgtgcagg
      661 aaaattatct tcggagagga tgtatccgcc agcgcacgtt ctgtttcctg taacaacagt
      721 ttgtcatctg ttttacggga agcgaagctg cctttcttga ggaaagtggc atgctccgca
      781 tcagaagcaa taacagagat gcgtgtgtca tgctccccca gttttccttg attggatatc
      841 caccatgtgc tgtatcctgc ttttgctgcc agcgccacca cattgttgcc ggaatcaggg
      901 ttctgctcat agtcataaat cagtgtccgg ctcagggaag acactgtact ggaggctgtc
      961 gaggtataat cgtcaataaa taaaccgggt gccgtattca gccacggtgt ggttggtacg
     1021 ggatagtcat acactgacat ataatccctg cgcacactct caccagtgac aataacaatc
     1081 gtatcatata acggtgctcc cggctggatt ttccagttgt cagccccgtg ctactcagtt
     1141 gttcataacg ctgcatttca cgtaatgtgt cagttgtccc cacaactgtt cctttaacca
     1201 tccgcaatgg ccaacggttt acagagcata atacgaatag ccgtagagcc agccagttac
     1261 ggtggtcgcg atggtgtgtc cgccagaaaa tcaccacgaa taccagaatc gcggcactga
     1321 ccagaaaatg ataaacagga aacacggggc ggttcacttt gctttctcct gtagtgtttt
     1381 ctgtaggttc tgaaccggtt ctagcggggt tgtgaggctg atggcaatcg ccgggctctt
     1441 cctgcatgtt acactggtac tgcaagaggg ggccttcgct cctctgacat tactcagtat
     1501 tttcactact acaccccatc tgccatccat aacaaccgat tcaccccctc gcgaggcgtt
     1561 gttgcccgtc acccggacag acaccgttat ggatttctct tgttgcacga tcgatcaatt
     1621 gttatgcatg ctgacaccac aaccccggtg ggtatgaccg atgcctttct cactctcttc
     1681 ccggtcatcg tcggccttcc ccgttattgt ggcgtgtcag caccctcgac ggcatttcga
     1741 tggctgctca gtgttcactc acgttacggc ccgcataacc cgttgacttc ctgaagaagt
     1801 cgtttcaggg agagattcag cccttttgtt acctcctgga ccgcttcttg tccttccagt
     1861 cggagcgaga gttagccggg gcggactttc accgacggaa agatcgtgca tttcaaggca
     1921 cacacaacaa tatctgcgag cgtgctatcc gtccggtcgt tatgggacga aaggcctggt
     1981 tgttcgctgg ttcactggtg gccgggaacc gcgcggcaca gataatgagc cttctgggaa
     2041 ccgcagtctg gagccacatg cttggctgac ggacgtcctg acgcgtctgc cggagtggcc
     2101 ggaggagagg ttggctgagt tactgcctct tgagggcttt accttcttcg ggtaagtgat
     2161 acctgccgtc aggtgtttgt gcaccgggcc ataacctgca gttgggaatt gaacgcctga
     2221 caacaggaaa tgagctagag cagtaaacac gcgggctgac aatgggtcag aatatgagtt
     2281 agcccggcgt tattcctggg gtcagtactt cagatctagc ctccggtacc agactagcta
     2341 gcccctacgc aaccaaataa atcaaccatc gtcagagtgc aatcgttcat atacactgtc
     2401 ggtgatgcgc taaaaaatgc gttaatgttg agttcctcga acccttacca attaagggac
     2461 atagccagat acgcaactgg ctgatgcaag cagtctaatg tctttagatc cgctggcctg
     2521 cgggcatgac aatgtcaaac agtcaaagat tgtggcgttg acggtcagtc ttggggacaa
     2581 aaaagctcag tattctcgtt ggcaaccaga taatttaaca gttgatgctg aaacgaaata
     2641 ggcatcttgg ttc
//
LOCUS       DAZFRG010000396         2623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFRG010000396 DAZFRG010000000
VERSION     DAZFRG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.398"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..158)
                     /locus_tag="ACHKUC_004891"
     CDS             complement(<1..158)
                     /locus_tag="ACHKUC_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO6620962.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTI"
     gene            402..659
                     /gene="ynaJ"
                     /locus_tag="ACHKUC_004892"
     CDS             402..659
                     /gene="ynaJ"
                     /locus_tag="ACHKUC_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO6620963.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(709..1659)
                     /gene="uspE"
                     /locus_tag="ACHKUC_004893"
     CDS             complement(709..1659)
                     /gene="uspE"
                     /locus_tag="ACHKUC_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO6620964.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1811..2563)
                     /gene="fnr"
                     /locus_tag="ACHKUC_004894"
     CDS             complement(1811..2563)
                     /gene="fnr"
                     /locus_tag="ACHKUC_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO6620965.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          677 a          613 c          621 g          712 t
ORIGIN      
        1 attatggtgt agattaaaaa ttgcaccgca tggaataaaa atccttttct tttacgattt
       61 ccacggcgaa accaaaagct catcagaatc aatgctgcgc agctaccgaa aataatgacc
      121 agattaagcg cattatttgt aaacagttca gcgatcattg ttttatcagg ctcctccaga
      181 taattgtcgt catgccggaa acccctggcg ggactatttt accgcgacaa ttcattcaga
      241 tcatcaatag tcagggaagg aagtagcaac attagctaac taatcaatgc gttgattgta
      301 aatccagcta agaggtgaga ttttcagagc agacaacggt gaaatgtcat ggtattgtta
      361 cgtttaggta acaagaaatt tatctgcaca aggattacat catgattatg gcgaaactga
      421 agtcagcgaa agggaagaaa tttctctttg gtttgttggc ggttttcatt attgcggcgt
      481 cggttgtgac tcgcgcgacc atcggcggcg ttatagaaca gtacaatatt ccgctgtctg
      541 agtggacgac atcaatgtat gtgattcagt catcgatgat ttttgtttat agcctggtct
      601 ttactgtgtt gctggcaatc ccgttgggaa tttatttcct tggcggcgaa gagcagtaag
      661 taaaaaatag gcccgataac tcgggccttg tcagttattg aagagtcgtt aatcgtcttc
      721 ttcgtcatcc agttcaacgg gtgtctgata ctggtcaggt ttaatgacca gcaggtcgca
      781 gcgaagatga tcaatcacct gttccgccgt gttgccaagg aatgctgctg aaataccggt
      841 gcgtccgacc gtgcccagaa ccacaatccc cgcctgtaag tgctccgcca aatcaggaat
      901 cacctcttcg ggcagacctt tttctacgtg cgtcatgttt tcattaatgc cgaatttctg
      961 ccgcagagct ttcattgcca gcaaatgttg cccacgaatg gcatcgttat aaacgctcgg
     1021 gtcaaattcc ggcagttcaa tcgcgatatt aattggcgtt accggataag cgccaaccag
     1081 atgaacttcg gtatggttga cttgttctgc cagttcgatc gtctctttga ccagtttttc
     1141 attgagcgca ttatggtacg gctcttcact ggcgagattc accgccacca gcgccttacc
     1201 cccttccggc cacggctggt ctttcaccat ccacaccggg cttgggcatt tgcgtaacag
     1261 atgccagtcc gttggcgtaa aaatcaccgc ttccagacgg tcatgttggt gcgccatttt
     1321 tagcaccaaa tcgtgcccgc cgctgatcac ttcctgaatg atggcttcga aaggacggtt
     1381 atgccagacc actttaattt caatgggaac gccagcattg agataatatt ttgcctgctc
     1441 gtggatccag gctgtacgct ggctgatgac gccctgacgc atagcggtac gttcgtccgg
     1501 agagagcagg gtggtcattt cgtatgagaa gtcatagatc ggcaaaaagg ctttaatttt
     1561 gccaccaatc cgttgatgta aataaacagc tcgccgcaat gctggttggt cgtcctggtt
     1621 aggatcgata acaacgagca tgttctgata catagccata cagggtctcc ttacaacaac
     1681 tgtcaacgca gtttgtaatt aaaagattaa cccatatctg gtgaatgaaa cagtgatgaa
     1741 ccttctgtca gatcaataaa tcagaaaaat ttaatgatat gacagaagga tagtgagtta
     1801 tgcggaagaa tcaggcaacg ttacgcgtat gaccagcaag ctgggccagc gcatcgttat
     1861 tttcgatagt gatgtattta cctttgactg ccagcatacc gcttttctgg aagcgaccca
     1921 gcagacggct gatggtttca accgtcaggc ccagatagtt accgatatca ccacgagtca
     1981 tcgtcaggcg gaattcacga ggggagaagc cgcgttgggc aaaacgacgg gacaggttgt
     2041 agatgaatgc agccagacgt tcctcggcat ttttcttcga caacagcagg atcatgtcct
     2101 gatcgccttt gatttcaccg ctcatcagac gcatcatctg ctgacgcaga ttcggcattt
     2161 taccagacaa atcgtccagc gtttcgaacg ggatttcaca taccatcgag gtttccagcg
     2221 cctgcgcgaa actcgggtga tgaccgctgc cgatggcatc aaatcccacc agatcgcctg
     2281 ctaaatggaa accagtgatt tgctcgtcgc cttgctcagt gatggtataa cttttaatcg
     2341 taccggagcg gatggcataa agcgatttta gttcatctcc agccttaaac agcgtctggc
     2401 ctttctgaat aggcttcttc cgctcaatga tattatcaag ctgatcaagc tcatgttcgt
     2461 tgagtgtgaa cgggatgcaa agctggctga tgctgcaatc ctggcaatgg atagcacaac
     2521 cgccagactg aatgcgccgt ataattcgct tttccgggat cataggtctg ctcaagccgt
     2581 aattgatatt tgtcaatttt aacatctttt tagggagcaa gta
//
LOCUS       DAZFRG010000397         2587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFRG010000397 DAZFRG010000000
VERSION     DAZFRG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.399"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1632)
                     /locus_tag="ACHKUC_004895"
     CDS             complement(<1..1632)
                     /locus_tag="ACHKUC_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620966.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLSHSRWLAGIFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQSGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTY"
     gene            complement(1753..>2587)
                     /locus_tag="ACHKUC_004896"
     CDS             complement(1753..>2587)
                     /locus_tag="ACHKUC_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6620967.1"
                     /translation="RGTTPESSGSYGFVRLEGDLLRTEVAGMSVTAGIYGAAGHSSVD
                     VKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARG
                     RGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGF
                     RLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTA
                     GSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAESYNSQATLNVT
                     F"
BASE COUNT          596 a          714 c          674 g          603 t
ORIGIN      
        1 ataagtgacc gattcgatgg tcccgaaccg ggtgatatta cgggcctggg tataagtcag
       61 atgtccccga cgaatcagcc gggacgcttc tgcgggatcg gttacaccgg gtactttacc
      121 ttcgcggatc ttattcttca tggtttccac agcgccggca tactgatcac gggggacttc
      181 cagttgcatg ggaccatgat ttcccatgta acgatactgt ccgttttgtc cgtcgaatgc
      241 cgcaccgacg ctacgggctg cagttgaaca atatttcgtc tggatttctg ttccgctgac
      301 cagtctgtct gctccatttc tggcattatt atcaccgagg atccgtgcgt ttttcagacg
      361 gactctgtcg acaatatggt ttccccgttc agcggcaaac ccatgtccct gccctccgga
      421 agcaaacatt ttctcatttg tactccaggt ttccggcagt ttcatgagaa tcagaccatt
      481 cgccgttgca tcggaaataa aaccggtgta agaacgttca taaatgtcct gacacagtgt
      541 ttctgtttcg cgacaaagag aggagcagta ccattccgga tccggcttat gattttccgg
      601 ggcatgatgt acttctgtca gcccgcagga ggcacagata cgaagctcat ccacgtggtg
      661 gaatgataca aaacggcggc cttctgaaaa ggggtaccag tcaccgccgt ccaggcaacg
      721 atcgagaaaa tcgtcattcg cttccggtga taaattcagg cggttccgga agagggtttt
      781 cagttcatca ggggtaaatg acaggctctc tctcagcttt tcgttgaaac ccgccatgtt
      841 tctttcatac caggcattgt ccagtagcca gtttccgtct gccagttcaa attccctcag
      901 ttctccccgg gaaatccgtt gtgaaatgag tgtcagccag atgcgctccg tcagcgtttc
      961 aggaaggctg taatcgtttt gcaaactgct cagccacgat ttactgttga gcaccggttg
     1021 ttcttccagt gtctggcgga tggcactggt cagtcttttc agcgcctgga tctggctggc
     1081 cgcccatagc agcgtcacta atgcagccag ccagtacaac aacccgcccc accacatcag
     1141 tgagggtgaa atatcgaact gcaatgcgat caatggtgcg tatgcagaac acgccataag
     1201 gaaaaaagca gtataaaaac agcagtaata cccgttaatt tccttgctga tatcttcagg
     1261 tgtgagcccg ctctgctgaa tggttcttat ctgcacggga agagccagca gaccagcggc
     1321 gagagcggtg gtcagaataa attttacaga gtcaaaagta tgagcctcca gtgcgatatg
     1381 tttaccatca agtccccaca tgaagctgat gatcattccg gcaatcgtca gaaatatgcc
     1441 tgccagccag cgactatggg ataaccgggt tatgcgttca gtgatatagc cagccagtaa
     1501 agccggaccg aacagacaca gcaatatgaa ccctgccagc gcagacgctg tgctggttaa
     1561 tgctctgaac aaaaggcgaa gaatggcaaa tcctgcgatc agcagaatca gcgcgccgac
     1621 tatctgtaac atagtcactc cgtgatgaat atcatgttat tgtgaatgcc agtgaatgtg
     1681 gcactgaaac gttttccccc gtccggatcc cggtcatgat gaccgggacc acagagaggc
     1741 aatgcagaat tatcagaagg tcacattcag cgtggcctga ctgttataac tttcagcgct
     1801 gctgccgttg atgctgtggg cataaccggc ctgaacgccc agggtgatat tttcccggac
     1861 acgggcttcc agtcctgcct gcagatccag tgaagtgcca ttctgtgagg gtgagaacgt
     1921 catcccgctg cctgcggtgg aggtccccac acgcatatca ccccgggagc tgaaggtgcg
     1981 gataacggaa ggctgtaccc accagttcac cggcagttca cgcacactgt gttttgcact
     2041 gtcacgcagg ggggcacggg atgaggtgcc ttcaccaaag gtcatatcgt tgtggctgcc
     2101 cagacggaaa ccggcacgca catgttgtgc actgccatgc ccgaacttca cataaccggc
     2161 gttgtcctta ccgtcatcca gcgaaagccc ctgccaggtg tactgcagcc gtggctccag
     2221 catcaggttg tcagtgatac tgaagggcag accggtttcc agtgagccca gccagccccg
     2281 gccccgtgcg cggaagtcgt tattgcccga tgacgctttc atactgtggc gggttccctg
     2341 tgccactatg tcagcccaca ggccggagga ggtgtgtgtc aggttcatat atccgcccag
     2401 gctgccggca tcatcccgga ccgtgccggc acgggagccg tcatcatcct taacatcaac
     2461 ggaagaatgg cctgcagcac catatatccc tgcggtcaca gacataccgg caacctctgt
     2521 tctcagcagg tcaccctcca ggcggacgaa gccatagctg ccgctgcttt ccggcgtggt
     2581 cccacgg
//
LOCUS       DAZFRG010000398         2555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFRG010000398 DAZFRG010000000
VERSION     DAZFRG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.400"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..534)
                     /locus_tag="ACHKUC_004897"
     CDS             complement(<1..534)
                     /locus_tag="ACHKUC_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6620968.1"
                     /translation="MSFSHSSLSAQVKSHLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQFGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQ"
     gene            640..1602
                     /locus_tag="ACHKUC_004898"
     CDS             640..1602
                     /locus_tag="ACHKUC_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620969.1"
                     /translation="MTAQQSDALREIANKARVTTILQCKAWKDTQRILKRSGLVCRER
                     SEPFDPEKHFDCYTVRYLYLLNIMALELKSDTRIKVEVGQWYRMTGKRLSLNVPPFML
                     IPRNIRRKVDGFRQSRQSEDEATKNPPQPFTGSLYKVLSRDSDSAELDAWFAEPPLTR
                     QEVWEGRRVTDFDPWALSSFICRSESPTFELFYQEYKRLGLKSLFVSGVMFEQFLTGL
                     SFRKYGDWVESQLLESLGNVMFFMLLYDMENLDKFIKELMDINVQSEDSKEKGKSRKE
                     RMLEYINSYIRNVYGRFLCTSKERYEQHKRKNSSKKKNGSGGTH"
     gene            complement(1599..2453)
                     /locus_tag="ACHKUC_004899"
     CDS             complement(1599..2453)
                     /locus_tag="ACHKUC_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory RepB family protein"
                     /protein_id="HFO6620970.1"
                     /translation="MSVHSPDESDHVFSRMEYEYYRHNDKSPSVNADNAGRDVVPEWV
                     QKDPVAAFWVWMFIYHVPDSIVRLSGEQYADYGTSCLYARLFATEAAPANHQARIRVT
                     RKWLQELIRLTGTENVSDFLYVVHFLWSYAEKVNPMTWLGSRRIHIKWAWAYLQKHCT
                     DEERHCPDISEFKASGESRLFIIAYVRRVFMKIYSVGTEPQKLKELVYQQNDFLRRMK
                     CSYQKMFRYEKTDSRVQISALISPEARNALRKMAKKNNLTQAEMLDKLILSADRLFMQ
                     KTAGDYSR"
BASE COUNT          727 a          547 c          603 g          678 t
ORIGIN      
        1 ctgctgacgg gacgggcatt tttctgtggc attccattct tcagcaggaa acacgcgatc
       61 ggcatggctg agaacaaaca gaaaacgcga gggatctgcg ccttcattca gtaaaaactg
      121 atgcatggca atatcagcag catacgcacg ttcatcagcc cgcaggatcc agataatcag
      181 atccagttcc ggtagcagct gacgataaag tgtccggtat tcctgatcat gctgcggtgt
      241 ttcaccaatg ccgggcagat cgaccagcgt cattctgcgt tcaccgaact gcagggtaag
      301 acgatgagcc tggcgggtgc agccgttcag aggatgtgtg gcgcagatac gggactgaaa
      361 aatggcatta cacagactgc ttttgcctgt tccggattta cccataatgc caatcacggg
      421 ctcgtaatga ataacactgt ggagatgttc aaggattttc tggcgaattt cttcaggaag
      481 aaacgtcaga tgtgatttca cttgtgcgga gagggaagaa tggctgaatg acataccgtt
      541 atcctgttga tttcgctgaa ccggatcaca ctaatccggc ttatcattca ggtaatatgg
      601 acgtgaaaaa atctggagtg acagagtgcg ggaggtgaga tgacggcaca acaaagtgat
      661 gcgctgcgtg agattgctaa taaggccaga gttaccacca ttcttcagtg taaggcctgg
      721 aaagataccc agcgaatact gaagcgcagc ggactcgtgt gcagagaacg gtcagagccg
      781 tttgatccgg agaaacattt tgactgttat acggtgcgtt atctgtacct gcttaacata
      841 atggcacttg agctaaagtc agatacacgg attaaggttg aggtggggca atggtatcgc
      901 atgacgggta aacgtttatc acttaatgtc ccgcctttta tgctgatccc gcgtaatatc
      961 cggagaaaag tggacggatt cagacaatcc agacaatcag aagatgaagc aaccaaaaat
     1021 ccgcctcaac cgttcacggg cagtttgtat aaggttttga gcagggacag cgattcggct
     1081 gagctggacg cctggtttgc tgaaccgccg ctgacacggc aggaagtgtg ggaggggagg
     1141 cgtgttacag attttgatcc atgggcgctg tcttcgttta tttgccgttc agaatccccg
     1201 acgtttgaac ttttttacca ggaatataag cgactgggat taaaatctct ttttgtatca
     1261 ggggtaatgt ttgaacagtt tttaacaggg ctaagtttca gaaaatatgg ggactgggtt
     1321 gaatctcagt tactggaatc actgggaaat gtgatgtttt tcatgctgct ttatgacatg
     1381 gaaaatctgg ataagtttat aaaagaactg atggacatta atgttcaaag tgaagattcg
     1441 aaggaaaaag ggaaatcacg aaaagagaga atgctggagt atatcaactc atatattcgc
     1501 aatgtttatg gaagattcct ttgtacgtca aaagaaaggt acgaacagca taaacgcaaa
     1561 aacagcagca aaaagaaaaa cggttctggt ggaactcatt aacgggaata atcccctgct
     1621 gttttctgca taaacagacg atcagctgat aagatgagtt tatccagcat ttcagcctgc
     1681 gtcagattat tcttttttgc cattttccgg agtgcattcc tggcttccgg agaaatcagg
     1741 gcacttatct gtacccggga atcggttttc tcataccgga acattttctg gtatgagcac
     1801 ttcatgcgcc tgagaaaatc attctgctga taaactaatt cttttaactt ttgaggttca
     1861 gtaccgacag aatagatttt catgaagaca cggcgcacat aagctattat gaacagacgt
     1921 gactcccctg aagctttaaa ttctgaaata tcaggacagt gacgttcttc gtcagtgcaa
     1981 tgtttttgca gatacgccca tgcccatttg atatgtatcc gccgggaacc aagccatgtc
     2041 atggggttca ctttttcagc atatgaccat aaaaaatgaa cgacatacaa gaagtcagat
     2101 acattttctg tcccggttag ccttataagc tcctgtagcc attttctcgt cacacgaatc
     2161 cttgcctgat gattagctgg agctgcttct gtggcaaaca gtctggcata cagacaggaa
     2221 gttccgtaat ctgcatattg ctcacctgag agtcggacaa tactgtcagg aacatgatat
     2281 ataaacatcc atacccagaa tgcggctaca gggtcttttt gcacccactc cggaacaacg
     2341 tctctgcctg cattatcagc attaacagaa ggtgatttat cgttatgtcg gtaatattca
     2401 tattccatcc gactaaaaac atgatcagat tcatctggtg aatgcacaga catgaactcc
     2461 ctgggcataa atgcacaacc aactctatag aactctttaa cccccctctt atcacctctg
     2521 tcagtaaatt tcgcctgaat ccccttttat ccata
//
LOCUS       DAZFRG010000399         2544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFRG010000399 DAZFRG010000000
VERSION     DAZFRG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.401"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(211..342)
                     /locus_tag="ACHKUC_004900"
                     /pseudo
     CDS             complement(211..342)
                     /locus_tag="ACHKUC_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            624..1982
                     /locus_tag="ACHKUC_004901"
     CDS             624..1982
                     /locus_tag="ACHKUC_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO6620971.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2221..>2544)
                     /locus_tag="ACHKUC_004902"
     CDS             complement(2221..>2544)
                     /locus_tag="ACHKUC_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6620972.1"
                     /translation="EQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKM
                     KTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRI
                     ALPAE"
BASE COUNT          638 a          647 c          670 g          589 t
ORIGIN      
        1 ggtaatacac acgctttcag acatttcggc tttcgtcatt ttggtatcat gggcatagct
       61 tctgctacct gaccatcata atcacgcagc ttcagtgaag cagtgaccac ctgtttaacc
      121 cggtgtatcg ttatgatctt atccagcaat aatggacacg cggctaagtg aaccgccccg
      181 ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt ttccgatgga
      241 agcataataa gctttttctg cttctgccgg tgggatatgg ccctgccttt ccagcaatcg
      301 tcgattgtta taccagtcca cccacgtgag tgtggtcagc tcatcatact gacacgtgca
      361 ttacccctac cgcgataatg caaccgtaca atatagggcg tttcctaaac gcagccctca
      421 tcactccaat ccaacaagac acagcatttt tgatcacgaa gaaagacaaa taatttccgt
      481 aaatgtttcc gttgttacaa ataacaacag tgttttacat ttctttggcc tcaggcccgc
      541 gcatgttgcc attcgatgtc attttcctga ctcgtttctt ctttatggtg tagtgtgatc
      601 ttcactttat ggatgctaac aatatgaaaa gaaaaattat tcccgtgcta atcggttgta
      661 cgctctcttt ctctgccctg gcggcacaac ctacggctga gcgctatgtc gtcagttttc
      721 cagaaggcac gcacgtgaac tacgccggcg catttgccag tgcattcccg aacggccttc
      781 cggttgggat aggttctggc ctgctgttca ccggtaaaca gggcgatgcc ctgacattcg
      841 caaccattac cgatcgcggt ccgaacgcgg attcaccgaa agaggggaaa aacgaaacta
      901 aaattttcgt tacgccagat ttcgcgccgc tgctgatgac gattcgtgta caaaacggca
      961 aagcggaggc catcgatccc cgtcctctgc atgacgataa aggcgcgatc aacggtctgc
     1021 cgctggcaag cgacgtgatt ggctccacca atgaagtggc cttcagcgac acgttacata
     1081 gactgaaggg ggataatcgc gggctggata cggaagggat cactccggat ggtaaaggag
     1141 gttactggct gtgcgatgag tatggcccgt tcctgattaa catcgacagt aaagggaaaa
     1201 tcctggcaat ccacggtccg caggcggcgg aaggggaaaa agcgattgcg ggcggtttgc
     1261 caaatatcct caaatggcgt caggcgaacc gcggctttga aggcctcacc cgtatgccgg
     1321 acgggcgtat tattgtcgcc gtgcaaagta cgctggatat cgacgctaag agcaagaaaa
     1381 aggcgctgtt tacgcgtctg gtgagcttcg atccggcaag cggaaaaacc gcgatgtacg
     1441 gttatcctat cgacagcgcg gcctacagca aaaacagcga cgctaaaatc ggagacatcg
     1501 tggcgctcga taaccagcac atcctgctga tcgaacaggg aagagataaa aacaacagaa
     1561 tgcgtaacct gatttacgag gtcgatctca ataaggcgag cgacctgtca ggcttcgaca
     1621 agccgggtga gtatccggag tttgatgatg agaaaaccct gtcacagcgc ggcattaccc
     1681 ttgcgcagaa aacgcaggtt gttgatctgc gctccttggg ctggcagcag gagaaggctg
     1741 aagggttggc gctgatcgac agcaaaacgc tggcggtggc gaacgataac gactttggcg
     1801 tgaaggtggc gatgcaacat ccggtcgagg gcaagacatt caaggattat cgggtgaacg
     1861 cggaaggaaa gctgacgctt gacgacaagc aggttgaaac gaccctcaga gtgaagccac
     1921 tggagaaacc ggagtctgac agtgaactgt ggattgtgac gctgccggaa gcgttgaaat
     1981 aagtttataa ggtacggaca gcagtatcgc ttagtttttc aaattcatgt atgtgaacaa
     2041 tattggggtt ccggcgtaga cccccaatga aagcgtcacc acgtgaaaac atgcctgcac
     2101 ggtttacacg tcatctcatg gtggcagccg gttaactttc ctgctacacc agtggaaaaa
     2161 ggccataagc agtccgaaca aaagtaagcg tacagcgagg gccgtattga cggggatgtg
     2221 ttattcagct ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt tgaccggcca
     2281 gtctgctatg acgccaggca catggcgaag gtagctttct ggatccacgt cattcagttt
     2341 gcacgtcccg atcaggctgt acagtagcgc tccccgctca ccaccatggc cagagccgaa
     2401 gaacagggtc ttcatctttt cacgcaacca gctttccagg gatttcaaca gcggtttcgt
     2461 ttttcgctga cgttcagcaa gccgctgctc tgccggcatt ccccttatat ccgcctctat
     2521 ggcgtacaac tgaccgatct gctc
//
LOCUS       DAZFRG010000400         2512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFRG010000400 DAZFRG010000000
VERSION     DAZFRG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.402"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1675
                     /locus_tag="ACHKUC_004903"
     CDS             <1..1675
                     /locus_tag="ACHKUC_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HFO6620973.1"
                     /translation="TNSLAAVWRNGQSELIPNALGKFLTPSVVCVDEDGMVLTGEAAR
                     DLQLIKPQNCASNFKRMMGTSKTLKLGGREFRAEELSSLILRQLKEDAENYLGEEVTE
                     AVISVPAYFGDMQRKATKAAATMAGLNVERLINEPTAAALAYGLHNKDDEHQFLVFDL
                     GGGTFDVSILGLFDNIMEVRASAGDNFLGGEDIVDILIDAYCSRRDLPENIEWREPTL
                     QRHLRIEAERVKRVLSVRDEATFSVEIEGRRYYWHLTTEKFEFLLQTFFERIHMPLER
                     AIRDAKINISQLDQVVLVGGTTRMPLIRKLVTRLFGRIPAMHLNPDEVIAQGAAIQAA
                     LKARHSELRDVVVTDVCPYTLGVDTSKSLGHTRESGYFAPVIERNRSIPCSRVRAFYT
                     AHDQQTEVNFKIYQGESRMVADNIFLAELSVNVPPKPAGDVRIDVRFTYDINGILDVD
                     ISVPLTGAKNSLVIEQNPGALTAEQIQQSLSKLSLLKIHPRDEQINQAFIARLDNLYQ
                     LTLAETRDWISNCSRHFSYLLEKQDPDQLADFRTKVEPTLDSLERERLQ"
     gene            1672..>2512
                     /locus_tag="ACHKUC_004904"
     CDS             1672..>2512
                     /locus_tag="ACHKUC_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6620974.1"
                     /translation="MNCWEILALQPGAEERDIKRRYAQLVKNCRPEDDPAAWQQLHDA
                     YEQALRYDDEDQWQEDEEEQPINMSRGIVHPRPVMTFRALNTFSGHPLPDVTIKSEGE
                     VTWQAVLDMLCEENEAIPEHAPMQLAKALSFLEKKDLSSRMCFEEALLLHLHRVFRPL
                     LTLAAAQTFRWHLVAGSRQPVIQTEINESCLLYYRIEKQITPFFASNLSPLEEMENGK
                     EMATIYQSLKDNEESLKWFDIAVLNKVAEFELSDNYMALLIECLSYSLRKEKMIRKWK
                     LTER"
BASE COUNT          726 a          518 c          594 g          674 t
ORIGIN      
        1 aactaacagt ttggcagctg tctggcgaaa tggacaaagt gaactgatcc ccaatgcgtt
       61 aggtaagttt ttaacaccca gcgtagtttg tgttgacgaa gatggtatgg ttttgacagg
      121 ggaggcagct cgtgacctgc aactcatcaa accacaaaac tgcgcatcaa attttaaacg
      181 catgatgggc acatcaaaga cgttaaaact gggcggacgg gaatttcgtg cggaagaact
      241 ctcatcgttg atattacgtc agctaaaaga agatgcagaa aactatttgg gcgaagaagt
      301 cactgaagcg gtgattagcg ttcccgcata ttttggtgat atgcaacgta aagcaacaaa
      361 agccgctgcg acaatggcag gcttaaatgt cgaacgttta attaacgaac caaccgccgc
      421 tgcactggcg tatggtttgc ataataagga tgatgaacat caatttctgg tattcgattt
      481 aggtggtggt acgtttgatg tctccatact ggggttgttt gacaatatta tggaagtacg
      541 tgccagtgcc ggggataact ttttaggtgg tgaagatatt gtcgatatcc tgatcgatgc
      601 atattgctca cgtcgggatc ttcctgaaaa tattgaatgg cgtgaaccga cactccagcg
      661 gcatttacgc atcgaggctg agcgtgtcaa acgcgtgctg agtgtgcgag atgaagccac
      721 tttcagtgtg gagattgaag gaaggcgata ttactggcat ctgactacag aaaaatttga
      781 atttttatta cagacctttt tcgaacgaat ccatatgcca ctggagcggg caattcgcga
      841 tgccaaaatt aatattagcc aattagacca ggttgttctt gtgggtggca ctacacgtat
      901 gccactgatt cgcaaacttg tgacacgatt atttggtcgt ataccggcaa tgcatttaaa
      961 cccggatgaa gtcattgccc agggcgcggc tattcaggct gcacttaaag cgcggcacag
     1021 cgaattacga gatgttgtag tgactgatgt ttgcccttat acgcttggcg ttgatacctc
     1081 taaatcgctg gggcataccc gcgaatcagg ctattttgca cccgttattg agcgtaacag
     1141 gagtattcct tgtagtcgcg ttcgtgcctt ttacacggca catgatcaac aaacagaggt
     1201 taattttaaa atctaccagg gtgaaagcag gatggtggct gataatattt tccttgcgga
     1261 gttgtcagtg aatgttccgc caaaaccggc cggtgatgtg cgtattgatg ttcgttttac
     1321 gtacgacatt aacggtattc ttgatgtcga tatcagcgtg ccactgacag gtgctaaaaa
     1381 tagtctggtt attgaacaga atccaggggc attaacggca gaacaaatac agcagtcgtt
     1441 gtccaaatta agcctgttaa aaattcaccc acgcgatgaa cagataaacc aggcctttat
     1501 tgcccgtctt gataatctct atcaactcac cctggcggaa acgcgagact ggataagcaa
     1561 ttgctcccgt catttcagtt atctactcga gaagcaggat cctgatcaac ttgctgattt
     1621 tcggactaaa gttgaaccta cattagactc tctggaaaga gaacgtctgc aatgaactgc
     1681 tgggaaatat tagctttaca accaggtgcg gaagaacgag atattaagcg tcgttatgcg
     1741 caactggtaa aaaattgccg cccggaagat gatcccgcag cctggcaaca attgcacgat
     1801 gcttatgaac aggctttacg ttatgacgac gaagatcagt ggcaagaaga tgaagaagag
     1861 caaccgatca atatgtccag gggtattgtt catcctcgtc ctgtgatgac atttcgcgca
     1921 ctcaacacct tttctggtca tccacttcct gatgtcacta ttaaatcaga gggggaagtc
     1981 acctggcaag ccgtgctgga tatgctctgt gaggagaatg aagctattcc cgaacacgcc
     2041 cccatgcaac ttgctaaggc gttatctttc cttgagaaaa aagatctcag ctcacgaatg
     2101 tgctttgaag aagcgttatt actgcatctt catcgggtat tccggccatt attaactctc
     2161 gctgctgcac aaacttttag atggcattta gtcgcaggca gtcgtcaacc cgtaatccag
     2221 acagaaatta acgaaagttg tcttctttat tatcgcatcg aaaaacaaat tacccctttc
     2281 tttgccagta acttgtctcc actggaagaa atggaaaacg ggaaggaaat ggctacgatt
     2341 tatcaatcat taaaggataa tgaagagagc ctgaaatggt ttgatatcgc agtattaaat
     2401 aaagttgctg aatttgaact atcagacaac tatatggcat tgttaataga gtgtttatca
     2461 tattccttgc gaaaagaaaa aatgattcgc aagtggaaat taacagaacg ct
//
LOCUS       DAZFRG010000401         2470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFRG010000401 DAZFRG010000000
VERSION     DAZFRG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.403"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..1020
                     /gene="yiaK"
                     /locus_tag="ACHKUC_004905"
     CDS             22..1020
                     /gene="yiaK"
                     /locus_tag="ACHKUC_004905"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO6620975.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDNETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1032..1496
                     /locus_tag="ACHKUC_004906"
     CDS             1032..1496
                     /locus_tag="ACHKUC_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6620976.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTIDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKLVVKVALAAL"
     gene            1519..2439
                     /locus_tag="ACHKUC_004907"
     CDS             1519..2439
                     /locus_tag="ACHKUC_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HFO6620977.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT          655 a          617 c          610 g          588 t
ORIGIN      
        1 aaaaaaacaa ggaagcctgg gatgaaagtg acatttgagc agttaaaagc agcctttaat
       61 cgggtcttaa tttcacgcgg cgttgacaac gaaacggctg acgcctgtgc agagatgttc
      121 gcccgcacca ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt
      181 caacaactgg aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc
      241 ggcgcaattg aacagtggga cgcccagcgt tcgatcggta acctgacagc gaaaaagatg
      301 atggatcgcg ccattgaact ggctgccgat cacggtattg gtctggtggc actacgtaat
      361 gccaaccact ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt
      421 ggcatttgct ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc
      481 atcggcacca acccgctgat cgtcgccatt ccttctaccc caatcaccat ggtcgatatg
      541 tcgatgtcga tgttctctta cggcatgtta gaagttaatc gcctggctgg tcgtcagctc
      601 ccggtagatg gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag
      661 aaaaatcgcc gcattttgcc gatgggctac tggaaaggtt cgggcatgtc gattgtgctg
      721 gatatgatcg ctaccctcct ttctgacggc gcatcggttg ccgaagtcac cgaggacaac
      781 agcgacgaat acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac
      841 ggtcccaccc gcgatgccaa actgcaacgc atcatggatt acgttaccag tgccgagcgt
      901 gctgacgaaa atcaggctat tcgtttaccc ggccacgaat ttactaccct gctggcagaa
      961 aaccgccgta acggtattac cgttgatgac agcgtgtggg caaaaatcca ggcgttataa
     1021 ggagataagc catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg
     1081 ccattgaaaa ggcgctcgat tttctgcgca ccatcgactt caacgccctg gaaccgggcg
     1141 ttgtcgaaat cgacggcaag aatatttatg cgcaaattat cgatttaacc acacgcgaag
     1201 cggtagaaaa ccgtccggaa gtgcatcgtc gatatatcga tattcagttt ctggcgtggg
     1261 gtgaagagaa aatcggcatt gctattgata cgggaaataa taaagtcagc gaatcactat
     1321 tagagcagcg cgatattatt ttttatcacg atagtgaaca tgaatcattt atagaaatga
     1381 ttccgggcag ctacgctatt ttcttcccgc aggatgttca tcgcccggca tgtaataaaa
     1441 atgccactac ggcaatacgc aagctcgtgg ttaaagtggc cctcgctgct ttataaaaaa
     1501 tgattactgg agcacataat gaaaacgaat aatgccggtt atattatcgg cgcgtatccc
     1561 tgtgcacctt cttttcacca aagaagtgaa gaggaagaga aggatttctg gcgacaatta
     1621 tctgagactc cagacatttt ggggctggaa caaccttgcc tggaaaattt tcacccgctg
     1681 ggcgatcaat ggttactccg tcatacgcca gagagctgga aatttgtcgt caccgcagtg
     1741 atggaaacca tgcgtcgccg cagcgaaaac agcgggtttg gtctcgcctc cagtgatgaa
     1801 gaacagcgcc aggcctgcgt ggcgtactat cgccacctgt tcaataaaat caactcattg
     1861 caggctaata aagtgctcgc actggagctc caggctgcac cgttagcaac gaatcctaat
     1921 gttatgcaag cgacggacgc ttttgcccgc tcattgaaag aaatcgccag ttgggactgg
     1981 ccgtgcaagc tggtgctgga acactgcgac gccatgacat cttcttcccc ccgaaaaggc
     2041 tttttgcctt tagaaaatgt gctggaagtt attaccgatt atgacatcag catctgtatc
     2101 aactgggcgc gctctgccat tgaagggcgc aatacaacgc tgccgctgac ccacacccaa
     2161 atggcgaaac aagccggaaa actcggcgcg ttaatgttct ctggtacgac gctaaacggg
     2221 gcatacggcg aatggcagga tttacacgca ccgtttgcac cattctgcgc agaaagcctg
     2281 atgaccacag atcatgttcg cgagttattt aatgtagcag aatcgtcaac attgcatttt
     2341 gctggcatca agctactgga aataaatgcg acggctgatg tgcaccatcg catcgaaata
     2401 ttacgtaacg gcattcactc tctgaatgaa tctcgttaaa tagttccgct tatatatcac
     2461 tttgaatatc
//
LOCUS       DAZFRG010000402         2460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFRG010000402 DAZFRG010000000
VERSION     DAZFRG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.404"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..588)
                     /locus_tag="ACHKUC_004908"
                     /pseudo
     CDS             complement(1..588)
                     /locus_tag="ACHKUC_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016515.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(677..1111)
                     /gene="uspF"
                     /locus_tag="ACHKUC_004909"
     CDS             complement(677..1111)
                     /gene="uspF"
                     /locus_tag="ACHKUC_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO6620978.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1252..2385)
                     /gene="ompN"
                     /locus_tag="ACHKUC_004910"
     CDS             complement(1252..2385)
                     /gene="ompN"
                     /locus_tag="ACHKUC_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO6620979.1"
                     /translation="MKSKVLALLIPALLATGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNADFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          712 a          526 c          549 g          673 t
ORIGIN      
        1 aattgattat tataagcgcg aaaagaaatg ctacagcaaa taagaggacg attgtcggcg
       61 ctggggctct gtcgagaaaa tataataacc atacgcctga tattgttatt gatatcgtta
      121 cggcgataag taatgtctta tgaaagcgat gcgtgatgag gacagcaata gaccctggag
      181 ctattaatag tatatagaca gaataattcc gactgctttt aatgtggcaa aaatggtagg
      241 cgataccatg cagagtaaac cgtaatgtaa taatgatatc tgtaatccgc tgaattgtgc
      301 ctgttgatag tcaagacaaa aattcctgaa aaaacgatac ctataatggt atctttcttt
      361 atgatgctat tatctttcag atagccagtt gcaattgcac aagacagtcc tacaataaat
      421 gcaccaattc ccagcggcat acctataatc caggcataga actatgccag gaaacattgc
      481 gtggttcatt gcatctccta ttagcgccca gtcttttaat accagaaaaa ctgataataa
      541 tgcacagggg aacgatatta taatagcgat tatcaatgca taattcataa atttaaattg
      601 taatgattag aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa
      661 tgtgcgggcg ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag
      721 ctgcggcgtt agaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga
      781 tcatatgagc ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttctt
      841 caacatggac atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt
      901 gcgatttggc ttccgctttc agatcatcca ttgccggtaa ttctgcggaa taggctaaac
      961 ccagagaggc atagtagggc agcgaaggta ttaccgtcag gaaatgaacc tctgcatcat
     1021 caatctttgc ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa
     1081 tatcgatagg gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa
     1141 gtgtaacgcc agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca
     1201 aaggaggaag aataagcaga aaaagcccgc caaaacagcg ggcaggggga tttagaactg
     1261 ataaaccaga cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact
     1321 gtcatcttca tccaacaggt tgattttata gtcaacatag gtggacatgt ttttattgaa
     1381 atagtaagtc gcgccaacat cggcgtattt aaccagatct ttatcatcaa cacctgctgg
     1441 gttgtctgca ccaccggcag cgtgcaggtc acggccttta gacatcagga aagagactgc
     1501 cggacgcaga ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt
     1561 tgcaacagca taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt
     1621 tgccaggtaa atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc
     1681 accacctgcc gcagtatggt taacctggtc attggtgcgg tcagaagagg tgtatgccgc
     1741 accagcgcta aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccgtt
     1801 ttcatggcga acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt
     1861 gttaccttga tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagcatt
     1921 acgataagtc gcgacgccat tggcacgacc agtcataaag ttgtctgcat tggtataaga
     1981 gtcaccgcca aattcaggta gcatatcagt ccagccttcg atgtcgtaca ttacgccata
     2041 attacgtccg taatcgaaag aaccgtaatc tgcaaatttc agtccggcaa atgccagacg
     2101 ggtccatgac tggtttgttg aagattcggt gttgtttgcc tgaatattgt attcccattg
     2161 accgtagcca gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgagcata
     2221 gctctggtcg ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc
     2281 atacagatct aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgt
     2341 agcgagcagg gcaggaatta agagtgccag tactttgctt ttcattgaat aaatccttta
     2401 gttattttat ttgcctttca tccccatttt ggggagtgaa aacatcctaa aggagaagtt
//
LOCUS       DAZFRG010000403         2458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFRG010000403 DAZFRG010000000
VERSION     DAZFRG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.405"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..258
                     /locus_tag="ACHKUC_004911"
                     /pseudo
     CDS             157..258
                     /locus_tag="ACHKUC_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            376..1499
                     /locus_tag="ACHKUC_004912"
                     /pseudo
     CDS             376..1499
                     /locus_tag="ACHKUC_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611960.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    657..773
                     /locus_tag="ACHKUC_004912"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(2153..2386)
                     /gene="papI"
                     /locus_tag="ACHKUC_004913"
     CDS             complement(2153..2386)
                     /gene="papI"
                     /locus_tag="ACHKUC_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HFO6620980.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
BASE COUNT          648 a          545 c          638 g          627 t
ORIGIN      
        1 ctatcagttt ttgctgatga tctttttatc tatagaaaac atcagccaag ctggttggtt
       61 cagtaacgct ttcatctttg agcattttat aaaaaattct tgaattgtta aaatagatta
      121 aagtctggta acagcaaaag ttaaacgaga aatcaaaagg aggtcccaaa tctgaaaatg
      181 aagcatctgc gtgatgatgt tgcagcggga cgtcgtcggc tgtatatcag agcaatctgt
      241 ccatccctgc gtgaagcccc gttactttat tcgtacccct tataatgggg tgttagccag
      301 ccagacccgg catgattact gtgaaccgcc ccgggaatcc tggagactaa attccctgag
      361 aaagaggtaa acaggatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca
      421 gttcgtatgg ttctggaaag tcaggacgaa tatgactcac aatgggcggc aatttgttcc
      481 atagctccaa agattggctg tacgccagag actctgcgtg tctgggttcg ccagcatgag
      541 cgggataccg ggggcggtga tggtgggctc accaccgctg aacgtcagcg tctgaaagag
      601 ctggaacgtg aaaatcgtga actgcgccgc agtaacgata tccttcgcca ggcttccgct
      661 tattttgcga aggcggagtt cgaccgcctc tggaaaaaat aatgccactg ctggataagc
      721 tgcgtgagca gtacggggtc ggaccggtat gcagtgaaca gcatattgcc ccgtcaacgt
      781 attaccattg tcagcagcag cggcatcatc ctgataaacg cagtgcccgt tgtcagcgcg
      841 atgactggct gaagagagag atacagcgcg tatacgatga aaatcatcag gtgtacggtg
      901 tgcgtaaagt ctggcgtcag ttgttacggg aaggaatcag ggtggccaga tgtacagtgg
      961 cgcgcctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgcacta
     1021 ccgtcagccg gaaaaccgtt gctgcaggtg accgcgtaaa ccgtcagttc gtggcagaac
     1081 gtcctgacca gctgtgggtg gctgatttta cttacgtcag cacatggcag ggcttcgtct
     1141 atgtggcgtt catcattgat gtgtttgccg gatacatcgt ggggtggtgg gtctcatcgt
     1201 ctatggaaac gacattcgtg ctggatgcgc tggagcaggc gttgtgggcc cgccgtccgt
     1261 ccggcaccat ccatcacagc gataaaggct ctcagtatgt gtcactggcc tatatggagc
     1321 gattaaaaga agcaaaactg ctggcatcaa cagggagtac aggcgactcg tatgacaacg
     1381 cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt aagagctgga
     1441 aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat aacaatcgat
     1501 gattgctggg aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt
     1561 ccatcggaaa caatgatctg gcagcctgag ttcacagata aaacactctc caggaaaccc
     1621 ggggcggttc agtaatatct gatcgtaacg gacggacgct ggaattaacc gatgaccgca
     1681 gcacagagag taggggatgc ggacggtcaa agatcgtgga gttggatttt ataaatctgt
     1741 ctccagtagt agatgaactg aatacaaagt tgccagagag tggtctgtca ggcagggaat
     1801 cagaaaccgg atattattga ctggtacatt gtgtcgggct gtgtacgttc tccccggaca
     1861 gacagtctca gttcgtagaa tctataaatt actgctactg atgctgccgg ggaaaggtgt
     1921 aacgaaaaag cagcctccgt taccggacag caagaaggct gaatgaagtt tatggcgttt
     1981 gtattttgta atgtctggcc atgcagtaaa tccagacagg tttttattat catgtgaagt
     2041 attctgacat aaaatactaa atttctattt tgtgatgaat gctgcaaaat tgaatctgca
     2101 ctctgatgta acttttatct gtttcagtga agcatgccca cagattgagt tattaagttg
     2161 tggaagaaca gttctgcccc gcctgcttct ctcctttcag aagccagtat gttgccatgc
     2221 ctcgccttaa tggagagcgc tgaaccacac cttctttttc cagtaataac aggtaatagc
     2281 gtgcctgata atccgttacc gccagcgcct ccgcaatttc tgcggttttc cctccattat
     2341 gcctgttcag aaattccagt atttcattct tcatatattc actcatctca ctgtaacaaa
     2401 gtttcttcga ataataaaaa tcatgctctc tgttatcaac ggaaaggtat ttttattc
//
LOCUS       DAZFRG010000404         2452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFRG010000404 DAZFRG010000000
VERSION     DAZFRG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.406"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            184..909
                     /locus_tag="ACHKUC_004914"
     CDS             184..909
                     /locus_tag="ACHKUC_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6620981.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            867..1544
                     /gene="bglJ"
                     /locus_tag="ACHKUC_004915"
     CDS             867..1544
                     /gene="bglJ"
                     /locus_tag="ACHKUC_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO6620982.1"
                     /translation="MEHSRIKKRNVALIEKCVMSRIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1582..2370)
                     /gene="fhuF"
                     /locus_tag="ACHKUC_004916"
     CDS             complement(1582..2370)
                     /gene="fhuF"
                     /locus_tag="ACHKUC_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO6620983.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
BASE COUNT          641 a          545 c          606 g          660 t
ORIGIN      
        1 tatcatttga tgctaacctt ctgacacatg cagtggagtt gttgtgcagc aggagtatgc
       61 tgatatgaaa gtgaatgcta aggataattt attcgcttaa tctattaatt tgctggaaat
      121 atttaaggaa tgaatgcctt gaataaacca tcatgatttt tagacagtgg atgtggagga
      181 aatatgttgc caggatgctg caaaaatgga attgttatca gtaaaatacc tgttatgcaa
      241 gcagggttaa aagaggtcat gaggactcac ttccctgaat atgaaataat atccagcgcc
      301 tctgcggagg accttacctt attacaatta cgtcgttccg gattagtcat tgctgatttg
      361 gccggtgaaa gtgaagatcc acgttccgtt tgtgagcatt attactcttt aatctcacaa
      421 tatcgggaaa ttcactgggt tttcatggtg tcacgctcct ggtattccca ggcagtagaa
      481 ttgctcatgt gccctacggc gacgttattg tctgatgttg aacccattga gaatctggtc
      541 aagaccgtac gttccggcaa tacgcactca gagcgtatca gcgccatgct gacctctccg
      601 gcaatgactg aaactcatga ttttagctat cgctccgtca ttctcactct ttcagagcgc
      661 aaggtactgc ggctattagg taaaggatgg ggcatcaacc agatagcttc attgcttaag
      721 aaaagtaata aaactattag cgcccaaaaa aacagtgcga tgcgtcgact ggcaattcac
      781 agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga acttaacttg
      841 ccatctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag agaaatgtcg
      901 cactcataga aaaatgcgtc atgagtcgta tcggtattga gagtttattc agaaagtttg
      961 cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag gatgccatgt
     1021 cgcggatctc gtttgcagcg gtcatttttt ctttttctgc catgagaagt gcgcgcagag
     1081 agggattatc ctgcctgact gaactggcga ttaagtttcc gcgtacccgg cgtttagtta
     1141 ttgcggatga tgatattgaa gctcggctga ttggttcatt atcgccatca ccgctggacg
     1201 gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt ttgtcattaa
     1261 atggtgtacg tcaggcgacc gatcgactga acaatcagtg gtacattaac caaagccgga
     1321 cgttaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac tcaatgacac
     1381 aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa tttaatgtga
     1441 tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat attctgttat
     1501 gtatgcggca ttgcgaaaca agtaatgtgt tgcatcctta ttaatccgca tgatgccggg
     1561 tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct gcacatccgg
     1621 taagcgataa cgctggcagc aagtgcggcg caccagcagg ccgtcgcgta gcaccacggt
     1681 acgccagagt ggattatctt caccggtagt aagcgttttc tcaaaaaaga gggcatagcg
     1741 cagcgattca accgtcgcct cgccgagcag ctgtttcatc tcagtcagat accagttgat
     1801 caaataaccg gtattgctcc agataagttt gccgttaatc tctcccgttg cttctagtgc
     1861 ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct gcggcgaatg
     1921 tagtgttgcg tttttatctt cacacacatc gacccagaaa caggcggcgc gtccggtttc
     1981 gtgaaactca acgtggacat gttccggcga cacatctaat gctttttcct gagtcagcag
     2041 cgccagcatt aatggtggta ccatcaggcc gatataccat tgcgcccaca gtgagatcag
     2101 cggtttgttt tcgcggatca tcaaaggttg gttgcggtag atatgatcgg aatagaccgc
     2161 gagtagagaa cttagcgcat taggtgatga ccattgcgcc agcgtcatgg cgttaagtgg
     2221 ggcgggttca tccaggcgga taaactccag caaatgttca cgatgttcgg cgatcgtcgc
     2281 ccgcacggct tgtgcaagcc cagcatcctg cggctggaga tgcgttcgcc agataacatc
     2341 ttcatagagc ggtgcggaac gataggccat aatcgtgata gtaatctaaa tgataattat
     2401 tgctaatcct atcgataggt ttacccgata gcaaggggtt tctctggctt gc
//
LOCUS       DAZFRG010000405         2445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFRG010000405 DAZFRG010000000
VERSION     DAZFRG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.407"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..560)
                     /locus_tag="ACHKUC_004917"
     CDS             complement(<1..560)
                     /locus_tag="ACHKUC_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006534855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6620984.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDIYSGTVNALIKMLDTRT"
     gene            complement(602..862)
                     /locus_tag="ACHKUC_004918"
     CDS             complement(602..862)
                     /locus_tag="ACHKUC_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFO6620985.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            1411..2160
                     /locus_tag="ACHKUC_004919"
                     /pseudo
     CDS             1411..2160
                     /locus_tag="ACHKUC_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          679 a          528 c          587 g          651 t
ORIGIN      
        1 taagtacggg tatcaagcat tttaattaaa gcgttaacgg ttcctgaata gatatcatgt
       61 ggtggaagtc gatttaacat cagtgccccc tttagtgtag aacagactat aacggcgagc
      121 tcatccggaa gaagaactgt tttgaactct ccactattct gcccggaggt gataatatct
      181 ttaatcatct gtatctcctg atgtgccagt tgacttacag cctcctgtaa ttcaggagaa
      241 aactgatggc tgtcggatag catggcataa ataccgcaca tttcccctct ttcagcacaa
      301 ccagcaaatg catcaagata tgtcctgaga cgttgtacac ctgaagaaac attatggaga
      361 gttatattaa gtctatcaaa tacttctgtt ttgtattggc agtaggctat accaagatca
      421 gttttcctcg ggaaatggtg atgaatactg gcttttctga taccaatttc tttttccaga
      481 tctgcgtaac tgaaccccaa aaatccgcgt tgctggatca gagtatccgt tatgcgaagt
      541 atattctcat aagtagacac ggctttacct gttatgtatt aaagggacta ttatacatat
      601 tttagcgata gataatggca gttccatcaa gtttattttt tcctcccaat gaagtgattc
      661 ggaaatgaga acctcctgcc tgctccgctt tgtctttaag cgcagttgcc acctcttctg
      721 gtgtatatag attaccaaca gaaatatagt ctgacggcat caaaccaaca acgtcctgac
      781 gattaatttc atctgcagca gttgcgtagc ctgtcagaat gagaccaagg gcgacaatgg
      841 tagtgcagat aacataattc atgagcaatt tctctttatg tttgcgatac tacacatgct
      901 accttctgat tggtaggctt gcaagatgga tttatttaaa ataagaggga tgatttgtat
      961 caaattgttt tgtatttttg tgttttgtat cactgaggtg aagttgtaag atctggccct
     1021 ctgcgaggtt ggctctgtcg aaaggaacag gtgacgagag ttaacatcgt tgccttggct
     1081 gcatggggga tatgtagcta ccccctgata tttccgccac ggaagccaat atgaacaaca
     1141 ataacaatac gcctcgtttc cagtatgctt catgtcggat attttgtaag cgctatgtac
     1201 ttcccgggac gcttatcttt tagccgtagc tggtagtgca ataaaagcct ggcagcaatc
     1261 caggcctcta cgttctaaga atcaactgtc agtgcagagg aatgcgcacg acgctctcgt
     1321 tgttattggc ttttgccgtc gcaccgtcgt ttttcacggt gacaaacagc gcgtgcgctt
     1381 tgctatccgt cttatatcac tggtgctgac aatgtggatg aggtcgcctt taaaaaagga
     1441 catcgttata taacggtgat ctccgatcgc gatggtcgtg cgctggcctt aacggacgat
     1501 cgtggcacag agagcctcgc cggctatctt cgcacgctta ctgacgggca gttgctggct
     1561 atcaaaacgc tctcaatgga catgaatgca ggctatataa gagcagcgcg tatccactta
     1621 ccctgtgcgg ttgagaaaat cgccttcgac cgcttccatg tagcaaagca acttggcgaa
     1681 gtggttgata aaacccgtca gaatgaacat ccgcacctcc ctgttgaaag ttggcgtcag
     1741 gcaaagggga cccgcttcct gtggcagtac agcgataaat ggatgaccaa atccaggcag
     1801 gaaaagctga tatggctgcg tgcacagatg aagctgacaa gccagtgctg ggcgctgaaa
     1861 gagctggcaa aggatatctg gaacaggcca tggagtgagg aaagacgtaa tgactgggag
     1921 agatggctgg ctctggcggc caacagtgac gttcctatga tgaaaaacgc cgcgaaaacg
     1981 ataggaaaaa ggctttacgg gatcctgaat gccatgcgac accgtgtctc aaacggaaac
     2041 gcggaggcgc ttaacagtaa gatcaggcta ctgaggataa aagccagggg ataccgaaat
     2101 cgggagcgct ttaagctggg cgtgatgttc cactacggga aactgaatat ggcgttctga
     2161 gccttcccac catgatcagg gaaaacccat gtatcatcag gttattatgt gagctggtaa
     2221 atcagatttt gcatggactg aaatcatgct gttatttagg gataaagaac cggctttttg
     2281 tcccgccccc tcgccattaa tgaaaaaaag aatggaatgc tccctctgag gagaagggca
     2341 gtagaaaaaa gaacccgcca gcaaaaatat tggggatgaa cagctttcgc tactcagatt
     2401 gctggcgggt atagttcctc atgaaataaa aatactacat aatcc
//
LOCUS       DAZFRG010000406         2421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFRG010000406 DAZFRG010000000
VERSION     DAZFRG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.408"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            268..411
                     /locus_tag="ACHKUC_004920"
     CDS             268..411
                     /locus_tag="ACHKUC_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO6620986.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="ACHKUC_004921"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="ACHKUC_004921"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6620987.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="ACHKUC_004922"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="ACHKUC_004922"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO6620988.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPLRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDAKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHKUC_004923"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="ACHKUC_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO6620989.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          639 a          578 c          590 g          614 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgtc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggtcagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttacttt gttttatctt
      721 cgctccagtc gttcggatat ttgcccgccg catgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tcccctgcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcggccaac tgcgggaacg gcttgatgca aaggccgcgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgct gatgcgttgc ccgaaatcac cacgtttggt
     1141 ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
     1201 tgtgttgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaacta gcgcgagtag tgaagcccgg cggttgggtc
     1321 attactgcca cgccgggacc gagacactta atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctgaaaggtt ttacattaca gcagagtgat
     1441 gagttgtgtt atctgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gcctgaagtc tggcaagcac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc aataccgatc agtaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttaa cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcag tggttaccag taaccagaaa
     1861 ccgtgtcgac ggcgcggttc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgagaaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccgactgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa ggcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgcgat ggttttcgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tgttcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatca gctactcccc aaatgagggc
     2401 gcaaccttaa catattttga g
//
LOCUS       DAZFRG010000407         2418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFRG010000407 DAZFRG010000000
VERSION     DAZFRG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.409"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..764
                     /locus_tag="ACHKUC_004924"
     CDS             <1..764
                     /locus_tag="ACHKUC_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6620990.1"
                     /translation="TLIVSTDAYGTNIGKLVLDYNPKTKKIDSYNGELITIFADQFKP
                     DTIVQNTIDKWSAKLNKITQEVVGHSPVVLTREYGSSSSTGNLILDAMMEKTPDAVAG
                     FQNSGGMRADFPKGDITLGDVISTFPFNNDLIEMDLTGRDLKSLMTHATNLTNGVLQV
                     SKSVAVVYDSKKPFNQRLISFTINGKPVEDNQTYRIATHSFCASGGDGFEAFLNGKNV
                     KTIPGTTSAESIIDYFKNHKPVTPDLTKRVMDVAK"
     gene            805..>2418
                     /locus_tag="ACHKUC_004925"
     CDS             805..>2418
                     /locus_tag="ACHKUC_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6620991.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSAPLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVS"
BASE COUNT          743 a          519 c          520 g          636 t
ORIGIN      
        1 acacattaat tgtatctacc gatgcatacg gcaccaacat cggaaaactt gtgcttgatt
       61 acaatccgaa aacgaagaaa attgatagtt ataatggtga gttaatcacc atctttgcag
      121 atcaatttaa acctgacacc atcgttcaaa atactattga taaatggagt gcaaagctta
      181 acaaaataac acaggaagtt gtcggccatt ctcccgtagt tttaacacgt gagtatggta
      241 gttcctcttc taccggcaac cttattcttg atgcaatgat ggaaaaaaca cctgatgccg
      301 ttgccggatt tcaaaatagc ggtgggatgc gagctgattt tcctaaaggc gatatcacac
      361 tgggagatgt tattagcaca ttccccttta ataatgacct catcgagatg gatttgacgg
      421 gccgcgatct caaatcattg atgacgcatg caaccaatct aactaacggt gtgttacaag
      481 tttctaaaag tgttgcggtt gtctatgaca gcaaaaaacc attcaaccaa cggttaatct
      541 ctttcaccat taacggcaaa cccgtggaag ataatcaaac atatcgtatt gccacgcact
      601 ccttttgtgc cagtggtggt gacggttttg aagcattttt gaatggaaaa aatgtgaaga
      661 cgataccggg aacaacctcg gcggaatcta tcatcgatta tttcaaaaat cataaacctg
      721 tcaccccaga cttaactaaa cgagtcatgg acgtcgccaa ataaaatctc atttatcagt
      781 caatttattt aacaaggaat gcgaatgaaa acagcgcagc gtactttatt tgccgcaaca
      841 ttgctgacta ttttttcgac ctcagttatg gcgcaggatg tcacgattat ttataccaat
      901 gatcttcacg ctcatgtgga ttcctataaa gtaccgtatg tcgcagacgg aaaacgcgac
      961 attggtggct ttgccaatat atccacgtta gtaaaacaag agaaagccaa aaataaagcc
     1021 accttttatt ttgatgctgg agactatttc accgggccat atatcagtag cctgacaaag
     1081 ggcgaagcaa taattgatat tatgaatacc atgccttttg acgcagtatc aatcggtaat
     1141 catgagtttg accatggctg ggataatgcc ctccgacaat taagcaaagc gaatttccct
     1201 gttttattag gcaatgttta tcataaagag agtgagaaac ccttctggaa caagccctac
     1261 accatcctgg aaaaagatgg catcaagatt ggtattgttg ggctacacgg cgtatttgca
     1321 tttaatgaca cggtctcgga gctctctctc cagggactcg ataatgacaa taataaccga
     1381 tttgataaat cttccgcgcc cctcaagaat caggggattg aagcacgtga tgaagtgaag
     1441 tatttgcagc attatatcga cgagttacgt gataaggttg acctcaccgt ggcgctggtt
     1501 catgaaggcg taccggcgcg tcagtccagc attggcaata cagatgttag acgtgcgctg
     1561 gataaagata ttcaaaccgc aagcaaggtg aaaggattgg atattctgat taccgggcat
     1621 gcccacgtcg gaacgccaga accaatcaaa gtcggcaaca ccttaatcct ttcgactgac
     1681 agtggcggta tcgatattgg taaattaatc cttgatgtca accctacagc tcgtactcat
     1741 aaagtgaaga gctttgagct gaaaacggtt tatgccgatg aatggatacc tgatccaacc
     1801 acacaaaaag ttatcaatgg ctggaataaa aaactggcgg atcttgtacg ccaaccagtc
     1861 ggtgaatcat cgattgcatt aactcgtgcc tacggtgagt catcgcaact tggcaacctg
     1921 tttaccgatg caatgcttgt cgctgcaccg actgcacaaa ttgcgttgat aaactccggc
     1981 agtttacgtg ctgatataaa tgcaggcacg attacctttg gtgacattac cagtacattc
     2041 cccttcaaga atgaacttac tgaaatggat ctcagcggta aggatctgcg taacctactg
     2101 gaacacgggg catcgcttac caatggcatc ctgcaaatgt caaaaggcgc agaaatgcgc
     2161 tataccctgc agaaaccagt aggtcagcgc attgtatctt ttaaaattaa tggtgaagag
     2221 atcgttgata cgaatattta ccatgttgcg actacgactt tccttgcact tgggggcgat
     2281 ggttttctgg cattcaaaga agggaaaaat gtccaggtcc gcgccggaaa taacatgtcc
     2341 aatgtcgtaa tcgattattt taagaaaggc cacaagatta ctcctgcaca ggtaaatgaa
     2401 atgcgggtag aagtaagc
//
LOCUS       DAZFRG010000408         2400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFRG010000408 DAZFRG010000000
VERSION     DAZFRG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.410"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(17..724)
                     /locus_tag="ACHKUC_004926"
                     /pseudo
     CDS             complement(17..724)
                     /locus_tag="ACHKUC_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            842..1878
                     /locus_tag="ACHKUC_004927"
                     /pseudo
     CDS             842..1878
                     /locus_tag="ACHKUC_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc40 family transposase"
     gene            complement(1954..>2400)
                     /locus_tag="ACHKUC_004928"
     CDS             complement(1954..>2400)
                     /locus_tag="ACHKUC_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HFO6620992.1"
                     /translation="ISHEYPSCDECYASLLIYTGNTHPNEISIHLELEPTSINIKGDE
                     IITLSGKKRYAKNSYWMLSSENLVNSKDIREHLDWLLHKLGPKSSNIAKLQALENISM
                     GIDCTWRSLNWHGGPTLWPEQMKIMANLGLECSFDIYFVGNEDGNL"
BASE COUNT          693 a          528 c          451 g          728 t
ORIGIN      
        1 atgacgcagg gcaagattac gaaagcccgc tccccgcaag gactgacgcc aggtagtttc
       61 tgtccatggc tgcttttcgt atcttacgtc ttaaccctgc cctgaatagc gatatgccgt
      121 atccctaaaa ataattctgc tgagtttcct cttcttattt tgctgtcgtc ttcattcatt
      181 accacgtcca gacgccagta cagcttattc tccacgtacc agtggtttcg gattgctgtg
      241 gcgaacttct ctgcggttaa atcagcagaa ctgatataat atctgaccgt catttctggc
      301 tctttctttt gttctgttat tattgaacga aaggagactg ccatgcataa tttcttcagc
      361 cctttccatt caaacgtgaa atcaataagt tcatcaggga tatcgcagac aatgtgaaga
      421 cgaatttctt ctctgccatg acgcttttca ctcattgcat aactgtcatg ctctggatta
      481 tttaattctt tcagtggaaa tttttcctca aaggttttat taagccgccc ctgatttcat
      541 tttcagcgaa taaataatca cctccctgtt tttgtatctt ctctgcaata tctttctggc
      601 aacccatcgc atcagttgtg atgatttttc ctttaatgtc caacatgtaa agaagttcag
      661 ggatagctgt aatctcatta gatttctcat ccgtcctgat ctgtccgatg accagactgt
      721 gcaataaaat gcgaattatt tagtacaaaa agtgtacttc tgatgatctt tccttgccct
      781 tgtttattag gtatataccc gtgatcattg aagatgcttg atttttcagc aacagaagat
      841 catgctgtcc aacatgaaaa tattcattac tgagcaacaa aaagctgaac ttgaacgcct
      901 tcatgattcc agtcgtgacg ggcgagtcag agatcgtatt aaagccattc ttctggcttc
      961 cgaaggctgg agttctgcca tgattgctca ggctttacgc ctgcatcaga caacgattta
     1021 tcaccatatc agcgaattcc tgaataaagg aaaactgaag cccgaaaacg gcggctcaga
     1081 cagcaaactc agtgctgaac aaacggcctt ttcatcagcc agttatccga taatttattt
     1141 caccataccc gcgatgttat tgcgtttgtc acccggacat ggaacatcat tttcagcatt
     1201 ccggggatga ataagtggct gcatcgtaat ggtttcacct acaaaaaacc gtcaggcgtc
     1261 ccccataaat tcagtgaaga gaaacaaagg caattcattg aatattataa ggaactgaaa
     1321 acaactgtgg gtgatgagcc gattcttttt attgatggag tccatccgac tcaggccact
     1381 aaaatcagct atggctggat acgcaaaggc cagaaaaaag cagtaaaaac aacaggcagc
     1441 cgcacacgcc tgaatatcat gggggcgctg aatctgaaag cactgacgtc accgctaatc
     1501 tgcgaatata aaaccattaa tgaatataat gtatcccgtt ttttcaatga gatacgaaag
     1561 gtttatcctg attataacca gaaaatccac gttattctgg atggagcagg ttaccaccgt
     1621 tcacagttag tgaaagactg ggcggaagtg gtaaatatca ggctgcatta ccttccccct
     1681 tacagcccaa acctgaatcc gatagaaaga atgtggaaat taatgaacga acatgcccgg
     1741 aataatcgct acttcagcag tacgcgagag ttcagggaag caatatctgt cttttttaac
     1801 cagacactgc cggatatagc ggactcactg acatcccgaa ttaacgatca ctttcaggtg
     1861 ctcacacccg catcttgagg tttattgagt atataccata aataccgagt attttattgt
     1921 gctatttgtt tattaaatat ttgcgagcaa taattaaaga ttgccatctt cattgccgac
     1981 gaaataaata tcaaatgaac actccaatcc aagattagcc attattttca tctgctcagg
     2041 ccataaagtt ggcccaccgt gccaattcaa tgatctccag gtacagtcaa tacccattga
     2101 aatattttct aatgcttgca attttgcaat atttgatgat ttaggtccaa gtttgtgaag
     2161 tagccaatcc agatgttctc gaatatcttt cgagttcact agattttctg acgagagcat
     2221 ccaatatgaa ttttttgcat atcttttctt tcccgataga gtaataattt catctccttt
     2281 tatattgatt gatgttggct caagttctaa gtgaatactt atttcattag ggtgagtatt
     2341 acctgtgtaa atcaagaggc tagcatagca ctcatcacaa cttggatatt catgagatat
//
LOCUS       DAZFRG010000409         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFRG010000409 DAZFRG010000000
VERSION     DAZFRG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.411"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            633..1199
                     /locus_tag="ACHKUC_004929"
     CDS             633..1199
                     /locus_tag="ACHKUC_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6620993.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFAERIAECWQAITYNCGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            1459..2034
                     /locus_tag="ACHKUC_004930"
     CDS             1459..2034
                     /locus_tag="ACHKUC_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620994.1"
                     /translation="MNAIPYFDYSMAPFWPSYQNKIVGVLNRALREQSCSRVRRILLR
                     LPWEHDNAFNSRQTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            2103..2339
                     /locus_tag="ACHKUC_004931"
     CDS             2103..2339
                     /locus_tag="ACHKUC_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6620995.1"
                     /translation="MLTSITGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
BASE COUNT          596 a          564 c          558 g          681 t
ORIGIN      
        1 ctggtgaaca tcaattctct gtcatcactg attaactgaa tgcttacgct ttgataaggt
       61 atccatgatt ccgtcatgtt taacagcgca gaataaacaa tgaaattaac agagtgaatt
      121 cctgattatg tttgtttcgg gttgtattat ctaagatgca ggatgaaaat catgtcagtg
      181 gtatttttta ttctgctatc gtgagccgtg aatgttcatg tccttaagaa cagattgaat
      241 gagcgcatat aagtttattg cattggcctt gtacacggtt tttacaacca ctgagagcaa
      301 gtttgtagtt tatgatgtga ttggtcgcaa tatgtttctt aaccttctgg tcgtggtgtt
      361 ttatcgcgta ttttgcagta tttcgtgatg ttttattgag tctgtatttt ctttactcct
      421 cgtttatctc atctctttag caaataccat cagataatcc atttctttct gcataatgct
      481 gcgtatcgtt aataacccgt cgtattcatt ctgctacagc atgcctgata aataccatct
      541 gtaagttatt accgttttag atctgattat gagcgaaagc attaattcgc tcacagagct
      601 taaaacatca ttaactttca ggagtcatca acatgcctaa atcttacaca ccaaactggt
      661 tttttaccgc tttacttgac aatcacatca atcaaatgat ggcacgctat tcctgcctgc
      721 gggccttacg catggatttc ttctacagga aagatacgcc tgatttctta caacctgatc
      781 atcgctggct tgaattgcag ttgcgtatgc tgctggagca ggtggaacaa tttgaaaata
      841 tcgttggttt cttctgggtg attgaatgga cggctgatca tggttttcat gcgcatgtcg
      901 ttttctggat cgatcgtcag agggttaaaa aaatatatcc ctttgcggag cggatagcgg
      961 aatgctggca ggcaattacg tataactgtg gttcggcgca ccgctgtaca tatcagccgc
     1021 attatacata caacatcaac attcctgtgc gccacaacga tcctgaaagc atcgataata
     1081 ttcgcggtgc cctgcattat ctggcgaaag aagagcaaaa agacgggctg tgtgcctacg
     1141 gctgcaatga agttcctgaa cgtcctgcag cagggcgtcc tcgtaagcct cacttctgaa
     1201 agtttgaatc atgagtttcc tggcccggaa acacttcgtc ggtaaaaact tacctcgatg
     1261 attaatgatg tgaactgaag tcaacggaga tcattcaccc tgaacctgca tccggtgttc
     1321 tgtcccttgt cggttacctg atcttcctgt tctgtcccgg ttctttacgt attccatcaa
     1381 ccccatccgc aaaccataac acgtcagctc attcacgggc agggcgcgtt gtgggttgcg
     1441 cataatggag catatcttat gaatgctatc ccttatttcg actatagcat ggcacccttc
     1501 tggccatctt atcagaacaa aatcgttggc gttctcaatc gtgcgctgcg tgaacagtcc
     1561 tgctcccggg tacggcggat cctgcttcgt ctgccgtggg aacatgacaa cgccttcaac
     1621 agcagacaga cctggtttgg tatggatttt atcgaaaccg tcagtgcgct gatgaatgcg
     1681 aaacccggac gcgacctttg ctggctcctg acccgtcatc cggaaaagcc ggaataccac
     1741 gtggtgttat gcgtcagaca agagtatttc gacggccctg aactggatcg gctgattctg
     1801 gatgcctgga gcaacgtact gggcttcgca tcaccgggtg aagcagcacc gtaccagaag
     1861 cagatcaccc gggatgtggt gctggacagt cgttcaccgg actgcgaaga catcctcaaa
     1921 gacttgatct gggcgttcag tgatttcgcc cgcgatcgtc gtggtgtgtg cgatccggaa
     1981 gcccgttgcc tcgccggtaa tcccggctat cccggatctg caggactgtt ctgaaagctc
     2041 agttcagcag attcaccagc atttgcccaa cctccataaa tctcacagag gacatcctga
     2101 ctatgttgac ctcaataaca ggccacgaca gtgtattgct gcgtgccgac gatcccctga
     2161 tcgacatgaa ctacatcacc agtttcaccg gtatgaccga taaatggttt tacaagctga
     2221 tcagtgaagg tcattttcct aaacccataa aactgggtcg cagcagccgc tggtacaaaa
     2281 gtgaagtgga gcagtggatg cagcagcgaa ttgaggaatc acgaggagca gctgcatgaa
     2341 acgtgttgtg atgccggtac gctggcaatg tgcaaaatgc cggcgctggt attgtggaa
//
LOCUS       DAZFRG010000410         2398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFRG010000410 DAZFRG010000000
VERSION     DAZFRG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.412"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHKUC_004932"
     CDS             <1..153
                     /locus_tag="ACHKUC_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6620996.1"
                     /translation="CPGQVRQAVAVMPHAAKVLKQVMVNTGGNKEQVPAKRQNRGSRN
                     STDDGG"
     gene            178..465
                     /locus_tag="ACHKUC_004933"
     CDS             178..465
                     /locus_tag="ACHKUC_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6620997.1"
                     /translation="MSTRNIHVNTASYTILVAGREKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            583..1404
                     /locus_tag="ACHKUC_004934"
     CDS             583..1404
                     /locus_tag="ACHKUC_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6620998.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTCQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1700..2290)
                     /locus_tag="ACHKUC_004935"
     CDS             complement(1700..2290)
                     /locus_tag="ACHKUC_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6620999.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINEIC
                     QATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     DRYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTGIKSSKGIRIPVTKKSLPEINSVQNN"
BASE COUNT          654 a          514 c          575 g          655 t
ORIGIN      
        1 tgcccaggac aggtgcgtca ggccgtggca gtgatgcccc atgcggcgaa ggttctcaaa
       61 caggtgatgg tgaataccgg agggaacaaa gagcaggtcc ccgcaaaacg tcaaaaccgc
      121 ggctcccgaa actcaacaga tgatgggggc tgaaaaccag agagaggaga aagctggatg
      181 agtacccgaa atatccacgt taacaccgcg tcgtacacga ttcttgttgc cgggagggaa
      241 aaaaacaccg gggaagagtg ggacgtcctg gaattcagca gcctgactga gctgaaaaaa
      301 tatcgcaaaa gccacccgga aaagatggcc ttcagctata gctacgcgct cagccggggt
      361 gtggatacgc agttccgcca tatcaacatt gcggaagccg atcacttcaa acagttcctg
      421 cgtcagatta agcgtgccgg cctcgatatc cgggcgatct gctgaccgga tgtgatgaat
      481 atccctccta attgttgcaa tcttgttaat ggaaagcggg ggggatgata ttaagcggaa
      541 aattttttat taaatttatt ctttcataaa ggagtagctg ttatgcgatt agcttcccgt
      601 tttggtcggt ataattccat ccgtcgtgaa cgtcctttaa cggatgatga attaatgcag
      661 ttcgtgcctt cggtattttc cggtgataaa catgagtccc ggagtgaacg ttatacgtat
      721 attccaacaa tcaatatcat caataagtta cgtgatgaag gtttccagcc attctttgcc
      781 tgtcagagtc gggttcgtga tttgggacgc cgcgaataca gtaaacatat gttacgtctt
      841 cgcagggaag ggcatattaa cgggcaggaa gttcctgaaa ttatcctgct taattcacat
      901 gatggttcat ccagttatca gatgatcccc ggaatttttc gttttgtctg cacaaatggc
      961 ctggtgtgcg gaaataattt tggcgaaatc cgtgttccac ataaaggtga tattgtcggg
     1021 caggttatcg agggagcgta tgaagtgctc ggtgtctttg ataaggtcac tgataatatg
     1081 gaggcgatga aagaaattca tcttaacagt gacgagcaac atttatttgg cagagctgca
     1141 ctgatggtca ggtatgaaga tgaaaataaa acgccagtga cgcctgaaca aataattact
     1201 ccccgtcgtc gggaagataa acagaacgat ctctggacaa cctgtcagcg ggttcaggag
     1261 aatatgataa aaggtggatt gtcggggcga agtgcctccg gtaaaaatac caggacaaga
     1321 gccattacag gtattgatgg tgatatccga atcaacaagg cgttatgggt gattgccgaa
     1381 cagttcagaa agtggaagtc atgataatat aagtaaagcc ccgaaaattt ttcggggctt
     1441 tacttatatt atcattcttc ggcatcgctg tgtcgtctga tatacctcaa ttccatcaat
     1501 cggatttccg gttgtctgta aatattgtgc ccgcgaaaaa tggtctggca ggaggctgcg
     1561 cagttaccgg gtatatataa gatgggttcc cgcttcgcgg ggcttcggcc agtcagcatt
     1621 gtcactcata tgatattttt gtgtgtggcc ttccatgccg cttttgcggc ataaccggta
     1681 tctgacaatg tctgtaagat taattgttct gcacgctgtt aatttccgga agtgatttct
     1741 tcgtgaccgg aatccggata cctttactgc tttttatccc ggtataaatc cggtaaacct
     1801 ctgatgcgta ggcaagacgt ctctggttct ggcgttcggt cttcctgaat ccggcattgt
     1861 atgcaccaac ggcctcccag gagacgcccc attttttaaa ggctattgcc agataataag
     1921 caccggtata aatgttcatg cagggatctg ttgtcagatg ttccggttta attccatagc
     1981 ggtccagttc gttaaaatgc tgggaatcta cctgcatcag tccgctgcca tatcccgtta
     2041 ccggattaat accgatggca ttaacccggt aacgggattc tttccatgat attgctctca
     2101 gtaaatcagg atctattttg taatcccggc ctgcaagatc aaagcaatca gtggcctggc
     2161 agatctcatt tataaacatc aggcagatgg ctaacatcca ttttttcatt tttccacctc
     2221 tggtgacttt atccgtaaat aatttaaccc actccacaaa aaggctcaac aggttggtgg
     2281 ttctcaccac caaaagcacc acacactacg caaaaacaag tttttgctga tttacgtttt
     2341 aaatcatgta attaacaaac gctatttata tttaataatt ctgaattatt aaatagag
//
LOCUS       DAZFRG010000411         2395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFRG010000411 DAZFRG010000000
VERSION     DAZFRG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.413"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..528
                     /gene="eco"
                     /locus_tag="ACHKUC_004936"
     CDS             19..528
                     /gene="eco"
                     /locus_tag="ACHKUC_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO6621000.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(668..2314)
                     /gene="mqo"
                     /locus_tag="ACHKUC_004937"
     CDS             complement(668..2314)
                     /gene="mqo"
                     /locus_tag="ACHKUC_004937"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO6621001.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMIHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAVDSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          568 a          656 c          599 g          572 t
ORIGIN      
        1 atcggtgaaa acctataaat gaagaaggaa agcaaaaaaa tgaagaccat tctacctgca
       61 gtattgtttg ccgctttcgc caccacttcc gcctgggcgg cagaaagcgt ccagccactg
      121 gaaaaaatcg caccttatcc acaagctgaa aaaggcatga agcgtcaggt gattcagtta
      181 actccgcaag aagatgaatc taccctgaaa gtagaactgt taatcggtca gacgctggaa
      241 gtcgactgca atttgcatcg tctcggcggg aagctggaaa gcaaaacgct ggaaggctgg
      301 ggctatgatt attatgtctt tgataaagtt agctcgccgg tttccacgat gatggcctgc
      361 ccggatggca agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg
      421 cgttacaaca gcaagctgcc gatcgtggtg tatacgccag acaatgtgga tgtgaagtac
      481 cgtgtctgga aggcggaaga gaaaattgac aacgcggttg ttcgctaaac tgctgtgaag
      541 tgctgcaacc tgtaggccga ataaggcggt tacgctgcat ccgacatcta acgcccgagc
      601 cggttgcctg atccgacgct gaaagcatac gccacatccg gcaaaccatg ccggatgtgg
      661 cacatcatta caacgcaata tccgccactt ctttttgaac gggttgcggt ttcaactgcg
      721 gtttcggcgt actatctact gcctgcggct tgtcgtattt cagccccagc acttcgctgg
      781 tgtactgcaa ctcgcgttct gttgccgcta catcaccgtt cagcttgcgt ccataagacg
      841 gaacgatcgc tttcaacgta gcctgccatt gcgggctgga aacgcgatcg ccaaatactt
      901 tttccagcag attcaacata atcggcgcgg cggttgacgc ccctggcgat gcccccagga
      961 gcgcggcaat ggtcccttgc tggtcactga cgacttcagt acccagacgc agtacgccac
     1021 ctttatcggc atcacgcttg ataatctgca cgcgctgccc cgcttgccac aaacgccagt
     1081 cctctttttt cgcctgcgga tagtactctt tcaacgcttc aaaacgatcc tcttcactca
     1141 acatcacctg actcaccaga tatttcacca gatcgaaatt atccagccca acgtgtatca
     1201 tcggcatcac gttagaggtg gtggtggaac tcatcagatc ccacaatgaa ccgtttttga
     1261 ggaatttggt tgagaaggtg gcaaatggcc caaacagcac tacgcgttta ccgtcaagaa
     1321 cacgggtatc gatatgcgga accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa
     1381 ccttcgccag atggtgatta accacgtccg ggttttccga aacaaggaac tgtccaccca
     1441 ccgggaaacc ggcgtagtct ttcgcctccg gaatccccga ttcctgtaac agtttcagcg
     1501 ccgcaccgcc cgcgccgata aagacaaatt tggcgcggat gttctgtgca gtgccgtttt
     1561 tcagatcggc aacggtgacg gtccagctgt tatcgtcatt acgctttagg gcgcggactt
     1621 cgctgctgag ttgcagcgag aagttagatt tcttctgtaa ggaggcgatc aactggcggg
     1681 tgatctcgcc atagttcaca tcagtgccaa tttctgtgcg tgttgccgct actttctgtt
     1741 gcggatcgcg cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag
     1801 agtaacgcat accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga
     1861 aattaacgtt atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac
     1921 gcggagtacg cagcacaccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa
     1981 atgcttcgtt aatggcgact gctttttcaa tactgatgct gccgtcggca ttttgcgggg
     2041 tgtaattgag ttccatcagt gcagaatgcc cggttccggc gttgttccag ccgttcgaac
     2101 tctcctgcgc gacaccttcc aggcgctcca ccatggtcat cgaccattca ggctccagct
     2161 cgcgtaaata ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag
     2221 tttcctgctc ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg
     2281 ccatcgagaa gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc
     2341 tttattgcag gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatt
//
LOCUS       DAZFRG010000412         2387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFRG010000412 DAZFRG010000000
VERSION     DAZFRG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.414"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..374)
                     /gene="psiF"
                     /locus_tag="ACHKUC_004938"
     CDS             complement(54..374)
                     /gene="psiF"
                     /locus_tag="ACHKUC_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO6621002.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(493..1908)
                     /gene="phoA"
                     /locus_tag="ACHKUC_004939"
     CDS             complement(493..1908)
                     /gene="phoA"
                     /locus_tag="ACHKUC_004939"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO6621003.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSR
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2009..2269)
                     /gene="iraP"
                     /locus_tag="ACHKUC_004940"
     CDS             complement(2009..2269)
                     /gene="iraP"
                     /locus_tag="ACHKUC_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO6621004.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT          531 a          616 c          562 g          678 t
ORIGIN      
        1 atgaaatttc gggtgagcca ttttcgtagc tcacccgtta gcactatcag gtatcaggcg
       61 gctttcttga ggcaggcact cataaactta ttacgatcat cccctttcag agattgttgt
      121 gttgcttgat tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca
      181 ggcgcagatt tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc
      241 aacgcctgcg ccgtcgcctg ctgattacaa gaggtcatac gctgttgttg tggggttaag
      301 gttctctcgg cagcgccgac ggtggttaaa aaaaccagac cgaaaagcaa ggtaaccagt
      361 aatgtaattt tcatcgcacc atccctcttc atgttttaac catgagcgta tgcgcccgtg
      421 atctgtcatt aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg
      481 ccgggcgcgg ttttatttca gccccagggc ggctttcatg gtgtagaaga gatcggtctg
      541 gtcggtcagt ccaacaacat tggcggcatg cgggccatac gccgcaatac gcaactgact
      601 gccggtatgt tcttgtgaat cctcttcgga gttcccgtaa ctcatcacca tcactgcgcc
      661 atctttggta tttagcgcct gggtgaggcc cggagctttg gtatctggcg caactatctg
      721 gctggcatgg gcgtgatcag cggtgactat gaccaacgtg ttaccatcct ttttagcgaa
      781 ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg
      841 attcgcagcg tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaagccttt
      901 ctcatttcta ctcaacaatt caatggcttt gtcggtcatc tgcgccaggg tcggtacact
      961 atcattacgt tgcggattag gcgtacaggt gactgcgggc ttatctatat taccgtggta
     1021 cgttgctttc ggtccttgcc agcgcactgg catattgccg tcagcaaaca gtcctaatag
     1081 gggtttttgc tgattcgctt ccgtcaccga attcagtgag gcagcatcgc tcaccaactg
     1141 ataaccacgc gcctgtgcct gttcacgcag cgtttttccc tgccattcac cggcggttgc
     1201 cgtttcagca aaggtttttg cgccgccgcc aagcgtaaca tcggcacggg cgttaagcag
     1261 ctgttcggta atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact
     1321 ggtcgcactc ggaccgtagc atttgcgcga ggtcacatgc gccaccagcg cagcgggcgt
     1381 ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat
     1441 ttccagaatc gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cattataggt
     1501 tttgacacct gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt
     1561 gccggttttt ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat
     1621 acctttaaaa aagccgcccg caccttcggc ataattgcgt gcggcagtaa tttccgagtc
     1681 ccccatccca tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc
     1741 acgcagagcg gcggtctgat cacccgttaa gcggcgagca ccgccgggtg cagtaatatc
     1801 gccctgagct gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg
     1861 ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc
     1921 atgtacaaat acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt
     1981 tatgacagct gttgaaaaga ttaacttttt actgacgagg atgcctcaat aacttcttta
     2041 cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt
     2101 acagcgcccc ctctacctga tcaatcaacc gctgttgatc attttgcgcc atattgcgaa
     2161 gcattgccgt gacgataatc tccaaagctt ctacctgcgc gcacagttct ttcgactctt
     2221 cttctttttg ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc
     2281 cttatccaaa gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta
     2341 ttatttttat gacaaaggaa atactttgcg caaatatttt ttgtttg
//
LOCUS       DAZFRG010000413         2376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFRG010000413 DAZFRG010000000
VERSION     DAZFRG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.415"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..878
                     /gene="xapA"
                     /locus_tag="ACHKUC_004941"
     CDS             45..878
                     /gene="xapA"
                     /locus_tag="ACHKUC_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO6621005.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGGPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            939..2195
                     /gene="xapB"
                     /locus_tag="ACHKUC_004942"
     CDS             939..2195
                     /gene="xapB"
                     /locus_tag="ACHKUC_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO6621006.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2248..>2376
                     /locus_tag="ACHKUC_004943"
     CDS             2248..>2376
                     /locus_tag="ACHKUC_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO6621007.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVG"
BASE COUNT          500 a          515 c          640 g          721 t
ORIGIN      
        1 ttataaaaag gcgttagatt ccaccctaca aaaaaaggat atatatgtat caggctcagt
       61 tttctcataa cccactgtat tgcgtagata ttatcaagac ttataaacct gatttcacgc
      121 cacgagtggc ctttatttta ggttccgggc tgggcgcgct ggccgatcag attgagaacg
      181 cggtcgcaat ttcctacgaa aagctgcctg ggttcccggt aagtaccgta cacggtcatg
      241 cgggtgagct ggtgctgggg tatctccagg gggggccagt ggcgtgtatg aaaggtcgcg
      301 gacatttcta cgaaggtcgt gggatgacca tcatgacgga tgcaatccgt acctttaagt
      361 tgctgggctg cgagttgctg ttctgcacca atgcggctgg ctcactgcgc cctgaagtgg
      421 gggccggcag tctggtcgca ttgaaagatc acatcaacac catgccggga acgccgatgg
      481 tgggtcttaa tgatgaacgt tttggtgagc gcttcttctc gctggcgaat gcctacgatg
      541 cggaataccg cgcactgtta caaaaagtgg cgaaagaaga ggggttccct ctgacggagg
      601 gcgtgttcgt ctcgtatccg gggccgaatt tcgagactgc ggcggaaatt cgcatgatgc
      661 aaattattgg tggggatgtt gttggtatgt ctgtggtgcc tgaggttatt tcagctcgcc
      721 attgcgaact taaagtcgtt gcggtctctg cgattaccaa catggcggaa ggtctgagtg
      781 acgtgaagct ttctcatgcc caaacgctgg cagcagcgga actctcaaag caaaacttta
      841 ttaatcttat ttgcggcttt ctgcgcaaaa ttgcctgagt aaaacaacaa taaacggcct
      901 tactgagtga ggccatatga tgcgacaagg aaacgattat gagcatcgct ttgcgcttaa
      961 aggtaatgtc ctttttgcaa tattttatct gggggagctg gctggttacc ctcggttctt
     1021 acatgattaa tacccttcat ttcaccggcg ctaatgttgg catggtttac agttccaaag
     1081 ggatcgccgc gattattatg ccgggtataa tggggatcat cgcagacaaa tggctgcgtg
     1141 cggaacgtgc atacatgctg tgccacctgg tgtgtgcggg cgtacttttt tatgcggcat
     1201 ccgtaactga tccggatatg atgttttggg tgatgttagt caatgcgatg gcgtttatgc
     1261 cgactattgc gttatcgaac agcgtctctt attcctgtct tgcccaggca gggcttgacc
     1321 cggtgaccgc tttcccgccc attcgcgttt ttggtacggt ggggttcatt gtcgccatgt
     1381 gggcagtaag cctgctgcat ctggaattga gtagtctgca actgtatatc gcgtccggtg
     1441 cgtcattgct gctgtcggct tatgcgttga ctttgccgaa gattccggtt gcggagaaaa
     1501 aagcgactac atcgcttgcc agcaagctgg gtctggatgc cttcgtgctg tttaaaaatc
     1561 cacgcatggc tatctttttc ctcttcgcca tgatgctggg cgcggtgctg caaattacca
     1621 acgtttttgg caacccattc ctgcatgatt tcgcccgtaa tcctgagttt gctgatagct
     1681 tcgtggtgaa atacccgtcc attttactgt cagtttcaca gatggctgaa gtcggtttca
     1741 ttctgacgat accgttcttt ttgaagcgtt tcggtattaa aaccgtcatg ctgatgagta
     1801 tggtggcgtg gacgctgcgc tttggcttct ttgcctatgg cgatccatca ccaaccgggt
     1861 ttattttgct gttgctgtcg atgattgttt acggttgtgc attcgatttc ttcaatattt
     1921 ctggttcggt ctttgtcgaa caggaagttg attccagcat tcgtgccagc gcccagggcc
     1981 tttttatgac gatggtaaat ggcgtcggcg catgggttgg ctcgattctg agcggtatgg
     2041 cagtggatta cttctcggtg gatggcgtaa aagattggca taccatttgg ctgatgtttg
     2101 cagggtatgc tcttttcctc gcagtgatat ttttctttgg gtttaaatat aaccatgacc
     2161 ctgaaaagat aacgcatcgt tcagttacac attaaaagga ttcgcagctc tgttccttgg
     2221 ggctgctttt atgataaagg ttaattagtg aaagatattt tattttcttc tggcgttggt
     2281 tttggcattg gtgctttgtt tacaatcgtg cgattaccta ttcctgttcc aaatgtattg
     2341 ccgggtatat tatccatcgt gtttatgtat gtcgga
//
LOCUS       DAZFRG010000414         2372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFRG010000414 DAZFRG010000000
VERSION     DAZFRG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.416"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..667
                     /locus_tag="ACHKUC_004944"
     CDS             <1..667
                     /locus_tag="ACHKUC_004944"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6621008.1"
                     /translation="AVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGRSVT
                     KLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVL
                     TDRYPQDQIPGAYDGTVFPPNIEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLE
                     VACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLIDAEKAITDFMTAR
                     GYH"
     gene            complement(716..2215)
                     /gene="pitB"
                     /locus_tag="ACHKUC_004945"
     CDS             complement(716..2215)
                     /gene="pitB"
                     /locus_tag="ACHKUC_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO6621009.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            2152..>2372
                     /locus_tag="ACHKUC_004946"
     CDS             2152..>2372
                     /locus_tag="ACHKUC_004946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621010.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKSVLFLVYQTISIHSKPLEII"
BASE COUNT          648 a          604 c          569 g          551 t
ORIGIN      
        1 tgccgttatt ggtagcgatg gttcaggcaa gtcaacggtg tgtgaacatc tcattaccgt
       61 tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaggccg gaaatgtcgg
      121 tcgttcagtg acaaaattac cgttgatggg aaaatcctta cataaaacaa ttgaacgaaa
      181 tcaggtgaaa acagcaaaaa aattgccagg accagttccg gcgctggtaa ttacagcgtt
      241 tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc gcgggttaat
      301 tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg gtacagtgtt
      361 cccacctaac attgaaggtg gtcgttttgt ctcatggctg gcaagccagg aacgtaaagc
      421 gtttcactgg atggcgagcc ataaacctga tctggtcatc aaactcaatg ttgaccttga
      481 agttgcctgc gcgcgtaaac ccgaccataa acgggaatcg ctggcgagga agattgccat
      541 aacgccacag ttaacctttg gcggtgcaca actggttgat atcgatgcta atcagccact
      601 ggaaaaggtg ctgattgatg cagaaaaagc gattacggat tttatgaccg cgcgtggtta
      661 tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag gaaggttaaa
      721 tcaactgcaa tgctatccag tacaacccac cagaaagaaa aatcgccgcc ggtaaagtaa
      781 acacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg ccgtccacca
      841 ccatcgttcc tgctacggca gatgagagta cgtgtgttgt ggagacgggc atcccaatat
      901 aactggcaag gccgatagac actgccgccg tcatttgtgc cgccatgcct tgcgcatacg
      961 tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc cagccaatca
     1021 tggtgccaat gccgagcgcc agtgctactg ccatgatgat ccataccggt gcgtactcaa
     1081 tggtgcttaa catatcgctg cgcagttttt tcagcaggtt ctggtcttct ttactgacgc
     1141 ctggcagttt cgccagcttc gcggaagtat cagagatgca cagcataatg cggcgcagct
     1201 ggctgcgctg actcacgctt aacggctcgt agctttccat attgcctggc agcatcgttt
     1261 taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactcc gtcacttgcg
     1321 cgccatcagt cgatgccgca ggcaatggag gttccatcgc aatcaacttc tgcggcagtt
     1381 caggatgctg ttgcaggtag tgttcgaagt tggtgacggc atcgcgggta cgggtaattt
     1441 catagccgga cgcgttcata ttgacgacga agccagcagg ggcaatcccc accagtacca
     1501 gcattaccag gccgatccct ttctgcccgt cgttagcgcc gtgcgaaaac gccacgcccg
     1561 cagcggaaac aatcagcgca atacgcgtcc agaatggcgg cttacgtttg ccttttttct
     1621 ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtcccg ctccagaaac
     1681 ggcgcagcag gaaaatcaga ccacccgcaa tgaccaggcc gacgataggg gaaacaatca
     1741 gcgaggagaa aattttggtc acttcacgca ggtttaacgc atccatcacc gatgagccgg
     1801 ttaacagcgc gttggttaaa ccgatgccga taatcgcacc aatcaaggtg tgcgaactgg
     1861 aggccggtaa accaaagaac cacgttccca ggttccagat aatcgccgcc agcagcatgg
     1921 aaaagaccat cgccaggccg tgagttgacc ccatattcag caacaaatcg gttggtaaca
     1981 tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa aaattaaaaa
     2041 atgccgccat caccacagca agttgtggtt gcatggcacg agtataaata acggttgcca
     2101 ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca aatgccagag
     2161 caagtaataa caaaagccct gtgtatatat caaggccaac aaataaattt agcatatatt
     2221 agattacgcc attttgaatt tacgaacgga cgcattatca gtgactttaa cggcatgggc
     2281 aaagtgaaat atcatttttt aacgataaaa agcgtgttat ttcttgtgta tcagacaata
     2341 agcattcatt caaaaccttt agaaataatc aa
//
LOCUS       DAZFRG010000415         2360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFRG010000415 DAZFRG010000000
VERSION     DAZFRG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.417"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2135
                     /locus_tag="ACHKUC_004947"
     CDS             <1..2135
                     /locus_tag="ACHKUC_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6621011.1"
                     /translation="PCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVTTNRSA
                     SNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSR
                     VLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITK
                     KGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPN
                     TNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKV
                     GVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDK
                     QYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYY
                     ESEQSTVSLFAQNDWRFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARES
                     ASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAE
                     HSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDN
                     IDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTL
                     VMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNN
                     LTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT          680 a          526 c          538 g          616 t
ORIGIN      
        1 taccttgtcg aatttatatc ctttccactc tgtcattatg catttctggg atagtttcta
       61 ctgcaaccgc aacttcttca gaaacaaaaa tcagcaacga agagacgctc gtcgtgacca
      121 cgaatcgttc ggcaagcaac ctttgggaaa gcccggcgac tatacaggtt attgaccaac
      181 aaacattgca gaactccacc aatgcctcca tagccgataa tttgcaggac atccccggag
      241 tagagataac agacaactcc ttggcaggcc gtaaacaaat ccgcattcgt ggcgaagcat
      301 cctcccgtgt tttaattctc attgatggtc aggaggtaac ttatcagcgc gccggagata
      361 attatggtgt gggactgttg atagatgagt ctgcgctgga gcgtgttgag gtagtgaaag
      421 gtccatattc cgtactgtac ggttcacagg caattggcgg tattgttaac ttcataacca
      481 aaaagggagg tgacaaactt gcatctggag ttgtgaaagc tgtttataat tccgcaacag
      541 caggctggga agaatcaatc gcggtccagg ggagcatcgg tggatttgat tatcgcatca
      601 acggtagtta ttctgatcag ggcaatcgtg atacgccgga tggacgtctg ccgaatacca
      661 actatcgtaa caatagtcag ggtgtatggt tgggttataa ctccggaaac catcgttttg
      721 gcctctcgct tgatcgctac agactcgcga cgcaaactta ctatgaggat ccagacggaa
      781 gctatgaggc atttagtgtc aaaataccta aacttgaacg agagaaagtt ggggtattct
      841 atgacacaga cgtggacggt gactatctaa aaaaaattca tttcgacgcg tatgagcaga
      901 ccatccagcg ccaatttgcc aacgaagtaa aaacgacaca gcctgttccc agtccgatga
      961 ttcaggctct gaccgttcat aacaagactg acacccatga taagcaatac actcaggcgg
     1021 tcacattgca gagtcacttt tcgctgcctg ctaataatga acttgttacc ggtgcacagt
     1081 acaaacaaga cagggtcagc caaaggtccg gtggcatgac ctcaagcaaa tctctgaccg
     1141 gcttcattaa taaggaaaca cgaactcgct cctattatga gtcagagcaa agtacagtct
     1201 cactattcgc acaaaatgac tggcgattcg ccgatcactg gacatggaca atgggagttc
     1261 gccaatactg gctttcttca aagttgacgc gtggtgacgg agtatcatat accgcaggca
     1321 ttataagcga tacctctctt gccagagagt ctgcgagtga tcacgaaatg gtaacatcta
     1381 caagcctgcg ctattcaggt ttcgataact tggagttacg cgctgcgttc gcgcaaggct
     1441 acgtatttcc cacactctcc cagcttttta tgcagacatc tgcgggcggc agtgtcacat
     1501 acggaaatcc tgatcttaag gctgaacact ccaataactt tgaattaggt gcacgatata
     1561 atggtaatca gtggctgatt gacagcgcag tttactactc agaagctaaa gattatattg
     1621 caagtctgat ctgtgatggc agtatagttt gcaatggtaa caccaactcc tcccgtagta
     1681 gctactatta ttatgacaat attgatcggg caaaaacatg gggactggaa ataagcgcgg
     1741 aatataatgg ctgggttttc tcgccatata tcagtggcaa tttaattcgt cggcaatatg
     1801 aaacttcaac attaaaaaca actaatacag gagaaccagc gataaacgga cgtatagggc
     1861 tgaaacatac tcttgtgatg ggtcaggcca acataatctc tgatgttttt attcgtgctg
     1921 cctctagtgc aaaagatgac agtaacggta ccgaaacaaa tgttccgggc tgggccactc
     1981 tcaactttgc agtaaataca gaattcggta acgaggatca gtaccggatt aacctagcac
     2041 tcaataacct gacagacaaa cgctaccgta cagcacatga aactattcct gcagcaggtt
     2101 ttaatgcagc tataggtttt gtatggaatt tctgatgaca aaaacaatcg ttatggaatt
     2161 ctttgtcctg atctgcccgc tttgcgggca ggctaacgaa cgtattgtgt ttgttgaagg
     2221 tttttcgacg ttgttttcca tctgggggtg aatatttatt cttcccgcca gcatgactct
     2281 atagtcattg ctacgacaat gacttataaa gctggttact gagcctatac ctttgtaatt
     2341 caaaagtgat atcagttcac
//
LOCUS       DAZFRG010000416         2325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFRG010000416 DAZFRG010000000
VERSION     DAZFRG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.418"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..410)
                     /locus_tag="ACHKUC_004948"
     CDS             complement(<1..410)
                     /locus_tag="ACHKUC_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6621012.1"
                     /translation="MQIVSSSLNEIGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLS
                     RVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFFRALQGVAGASMIPLVFTTAFIYY
                     QGKELGLAAAVVSALASLSPTLGPTLGGWITDNL"
     gene            complement(410..1300)
                     /locus_tag="ACHKUC_004949"
                     /pseudo
     CDS             complement(410..1300)
                     /locus_tag="ACHKUC_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            1397..2212
                     /locus_tag="ACHKUC_004950"
     CDS             1397..2212
                     /locus_tag="ACHKUC_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6621013.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT          650 a          573 c          482 g          620 t
ORIGIN      
        1 tcgaggttat cagtgatcca gccccccaga gttggcccta gcgtcggtga cagtgaggcc
       61 agcgcactga ctaccgcagc agccaacccc aactctttac cctggtaata aataaacgcc
      121 gtcgtgaaaa ctaaagggat catggatgcg ccagcaactc cctgtaaagc acgaaaaaaa
      181 atcatgatct gaatattcca tgcaagtcca caagcgatac tcattaaagt aaatattccc
      241 gcggatagag taaaaagcca acgagtggaa aatacccgtg acagccaccc agagagcgga
      301 ataacaataa tctcggcaat taaatagctg gtctgaagcc aggccatttc gtctttacct
      361 gcggacagac caccacctat ttcatttagg gatgacgata caatttgaat atcaagctga
      421 aactggctcc tgtcgctggg gaaaggcttt caatgtgacc gtgaaacact tcccctttga
      481 gaatatcagc acggatctca gccttcattc ctggcttcat tcccgctatt tggttttctt
      541 tataatttgc atcaacccac aactcactga ctggtactag tgaaactaac gatgtgccgc
      601 cttccaccca actgcctgta tgggcgctgc ggtttgcgac aatgccatca atcggcgcac
      661 gaacaagggt atattccaga tccagtagtg cctgagtcaa agcggcctga acttcttcga
      721 tacttgcacg tacgttttct tcctgagcag ataaaactgc cagccgttgc ttctccacca
      781 ggaaatcgtt cgcggctttg cgctcctttg ctgcgacctg ctggtagtca aacctggcgt
      841 tatcaataat ttgttgcgat attgccgctg attgtgcgag tttttcataa cgttcagtat
      901 ctcgcaggcg cttttgctcc tcatgtttta ctgcctgcca tgtttcactg gcactttgaa
      961 ttatcgactg ctgcatcgca atagtggctt gaatgctttc gagattggct ttgctacttt
     1021 ttatttttgc ctcaagcctg gcgacatttg cacgataatc ccgatcatcg agacgcagga
     1081 taatatcgcc tttttttacg ctctgattat cgcgcacctc tatagcagaa atataaccag
     1141 aaactttact tgccaccgtg gtgatattac caccaatgta agcatcatcc gtcgtctgta
     1201 taaatcttcc tttgcttacc caccagataa aaaagacaac gccagccaaa ataataccga
     1261 tcgctaccac cccaataagc tgttttttag aaattatcat cactctttac accgtatata
     1321 tccggaaact tgcatacgtc atatttaaaa tttaacatgg cttatttgat gatttctgac
     1381 aaaataccta ctgtaaatga caatcacact gcaacccaaa gcatcaccag ggcatcacat
     1441 tatcggactg gattcggaac atctgaatgg aggcacagtt ccctggcata aacatctcta
     1501 tgcccagttg ctttacccgg ctgaaggagt cgttcgtata tgggctggag aaagcgtctg
     1561 gctagtgcat gccagcagcg cgctttggct gcccccacaa atgccgcata agtttgttgc
     1621 gacaggcaac gtacttttaa aaaccgtact ggtttcagaa gcggaaagtg aaacgctggg
     1681 aaaagtttgc ttcatgacgg gtatttcacc cctgttaagg gaattactaa ttgcaattaa
     1741 ccaacttcca ccgtcgcaaa gtaccacaga taaacaacag ttacgttttt cagccctcga
     1801 gacactgata cttcaggaaa taaaaatggg ggtgaaaatg tcacttgaac tcccttggcc
     1861 aaatgatgag cggctacagc agctttgtga gaatttgcta aacaaccaag ggtatctgcc
     1921 aacactggat aatctagcgg ataaaattaa cgtcagtagc cgtacactca tgcgcttatt
     1981 tgttaaagag acgggattaa catttcgcca ctgggttcag caaatgcacg ttatttctgc
     2041 agtgacgctt cttgatgatg gatattcgtt gaccaaaatt gcccatcgtc tgggctatgc
     2101 ctcagctgaa tcgttcggca atatgtttaa gcgtcgaacg ggctactcac ccggtaaatt
     2161 tactcgtcga ttaacgatgc atgattatgc aatcacaaga caaatgattt aacttatatt
     2221 ttcgtgaaat ctgtcactga agaaaattgg caacggatgg gtaaaaccgt tataacactg
     2281 tcaccggtca ccaggacccc aggccggatt taagacgagg atgca
//
LOCUS       DAZFRG010000417         2320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFRG010000417 DAZFRG010000000
VERSION     DAZFRG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.419"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..2254
                     /locus_tag="ACHKUC_004951"
     CDS             113..2254
                     /locus_tag="ACHKUC_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO6621014.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPDGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT          646 a          549 c          545 g          580 t
ORIGIN      
        1 tgattctcaa ttacatatcc gatggcgaaa taacaaacgg atagtgctta gtgagcaata
       61 agaaacaatt ttcactcgcg acaatccgcg agtttttggg ataacagact aaatggctat
      121 gttcacacct tcattctcag gactcaaagg tcgggcgctc ttttcactgc tttttgcggc
      181 accgatgatt catgcaacag actctgtaac gaccaaagat ggcgaaacaa tcactgttac
      241 agcagatgca aataccgcaa ctgaggcaac cgatggttat caacctctga gcacctctac
      301 ggcgacatta accgatatgc cgatgctgga tatcccgcag gtggtcaata cggttagcga
      361 tcaggttctg gaaaaccaga atgcgacaac gctggatgag gcgctttata acgtcagtaa
      421 cgtggtacag accaatacat taggcgggac tcaggatgct tttgtacgcc gtggatttgg
      481 cgcaaaccgg gatggctcca tcatgaccaa cggtctgcga accgtacttc ctcgtagttt
      541 caacgccgca acagagcgtg tggaagtgct aaaaggcccg gcctccacgc tgtatggcat
      601 tctcgatcct gacggactga ttaacgtcgt gaccaagcgc ccggaaaaaa cattccatgg
      661 ttcggtttca gccacctcct ccagttttgg tggcggcact gggcaacttg atatcacagg
      721 tcccattgaa ggcactcagc tggcgtatcg tcttaccggg gaagtgcagg atgaagatta
      781 ctggcgaaac ttcggtaaag agcgcagtac atttattgcc ccgtcactca cctggtttgg
      841 tgataatgca acagtaacca tgctctattc ccatcgggac tataaaactc cgttcgatcg
      901 tggaacgatt ttcgacctta cgacgaaaca gcccgtaaac gttgatcgaa aaatacgttt
      961 tgacgaaccg tttaatatta cagatggtca gtccgatctg gcgcaactca acgcagaata
     1021 tcatctcaat agccagtgga cagcgcgctt tgattacagc tacagccaag ataaatacag
     1081 cgataatcag gcgcgtgtta ccgcgtatga tgcaacgaca ggaacactga cacggcgtgt
     1141 tgatgcaact cagggatcta cccagcgtat gcatgctact cgtgcggatc tgcaagggaa
     1201 tgttgatatt gccggattct ataatgagat tctgggtggg gtgtcatatg aatattatga
     1261 tcttctgcgt acagatatga ttcgctgtaa aaaagctaaa gatttcaata tatacaaccc
     1321 tgtttatggt aataccagca aatgtacaac ggtttcggcg tcggacagtg atcagacgat
     1381 caaacaggag agctactcag cgtatgcaca ggacgcactc tatctgaccg ataactggat
     1441 tgccgtcgcc gggatccgct atcagtatta cacgcagtat gcgggtaaag gccgtccttt
     1501 taatgtcaat actgacagcc gcgatgaaca atggactccc aaactggggt tggtctacaa
     1561 actgacgcca tcggtatcct tatttgccaa ttattcgcaa acatttatgc cacagtcgtc
     1621 aattgccagc tacattggcg atcttccacc agaatcatct aatgcttacg aagtaggggc
     1681 aaaattcgag ctattcgatg gtatcaccgc agatattgcg ctgtttgata tccataaacg
     1741 taatgtgttg tataccgaaa gtgttggtga tgaaaccatt gccaaaacgg caggccgtgt
     1801 tcgttcaaga ggggtagaag tcgaccttgc gggagcatta actgaaaaca ttaatatcat
     1861 tgccagctac ggctataccg atgctaaggt tctggaagat cctgattatg caggtaaacc
     1921 attgccgaat gttcctcgtc ataccggttc gctattcctg acctatgaca ttcataacat
     1981 gcctggcaat aacacactga cgtttggcgg tggtggacat ggcgtaagcc gtcgttcggc
     2041 aaccaatggg gctgactatt atctgccagg ctattttgtt gccgatgcct tcgccgcata
     2101 caaaatgaaa ttgcagtatc cggtcacact gcaattaaac gtcaaaaacc tgtttgataa
     2161 aacgtattac acctcttcca tcgccacaaa taatctgggc aaccagattg gcgatccgcg
     2221 tgaagtgcaa ttcacggtga aaatggaatt ttgatcaaaa aagcccggta tgtttaccgg
     2281 gctgagactg atgacaaact ccaaattgtc tgatgcgcta
//
LOCUS       DAZFRG010000418         2311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFRG010000418 DAZFRG010000000
VERSION     DAZFRG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.420"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            325..873
                     /locus_tag="ACHKUC_004952"
     CDS             325..873
                     /locus_tag="ACHKUC_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621015.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFFRQVHERITESNHTASPALPVVLRWLREQHGGATIRCLLLLSQASICH
                     LRVSATVDEECSQVVDLLQQAWRGINAGGQCLVERCFRVARGDISGQYVALKTVALSL
                     GLPVVTAITHRPVQRCTLITAQ"
     gene            complement(892..1406)
                     /locus_tag="ACHKUC_004953"
                     /pseudo
     CDS             complement(892..1406)
                     /locus_tag="ACHKUC_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258200.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            1933..2127
                     /locus_tag="ACHKUC_004954"
     CDS             1933..2127
                     /locus_tag="ACHKUC_004954"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6621016.1"
                     /translation="MMFCHGERSPYPYSMNRAVCFDNPALADKTCTELFHLVDMAVMD
                     GDEIMQYRKQSFSPLCNSLW"
     gene            2229..2311
                     /locus_tag="ACHKUC_004955"
                     /pseudo
     CDS             2229..2311
                     /locus_tag="ACHKUC_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          563 a          511 c          586 g          651 t
ORIGIN      
        1 tcagtaattt tgacttacat cacactttca tggtttaatc aggtgattta tttaatgaaa
       61 agagtgtgtt tgtggatgta ttgtgtatat gtcttaccag gcatctgttc ttacattaat
      121 taatatattg ctatttaagt attggtggta ttgtcagtaa cattcttttg gttaatttag
      181 ttattattcc tcactgatta attgtccttc cgttcttatt gtttgtaact tgtgtttgta
      241 tcgtaccttt caggtgttga tgcgcgcttt actgtgtctg taccgtcttg acaaaatcca
      301 acagggtttg aaaaggaaca tttcgtgcaa ataaccgaag ccttaatttc agagccggga
      361 gacatccggc gttttgttca gcaggcggtg gaccactggc cgcatttgct ggcattttac
      421 ttcacactcc attcggcaga aggaaatatc aacgggcaac agattcatgc attctgtact
      481 tcctttttcc gacaggtgca cgaacgtatt actgagagca atcacactgc cagtccagca
      541 ctgcctgtgg tattacgctg gttgcgggaa caacatggag gagcaacaat acgatgcctg
      601 ttgctgctca gccaggcgag catttgccat ctgcgggtca gtgccacagt tgatgaagaa
      661 tgttcgcaag tggtggattt actgcagcag gcctggcggg ggataaacgc tggcggacaa
      721 tgcctggttg aaaggtgttt tcgggttgcc cggggtgata tatccggtca gtatgttgcg
      781 ttaaaaacag tcgcattgtc tctggggtta ccggttgtga ccgccattac ccatcgtccg
      841 gtacagcgct gtacattgat tacagctcag tgaatcagcg cagttgtgta gtcaacgagg
      901 ttttctggct ttttgtcggt cattcagcac cattagccgg gtctcagcct ctttctgttc
      961 ctgtggagtc acttccccgc acggcagccc cttcaggtcg taacgggcgc cgccagccat
     1021 taacgcacgg taatagcgcg gagactacac ataagacgct actgccgcgc gtaatgcacc
     1081 tgcgccaaac gccagttctc tgatggcgat atcctgtatc aggtcgtcag gtatctcaac
     1141 tttaagcggt tggggttttt cccggttgaa taactcaggc cagtgttcca cgagacgatt
     1201 aatacgctta cggtttttac gctggcgttt tgtcatatgc cgccacggtg ttttttcagc
     1261 agagcagggc tctgtttatc gttcagctgt ggtatccgtt ccctggcctt cacgttgtgt
     1321 gcagtgttcg ttttatcctg cagcggcgtt gccggtgatt tactgaaaat gccctgtggt
     1381 ttacggttaa tggtcagcgt taccatcagt attgttacct catgctgtca atggaagtag
     1441 tactccatcc agagccttgg cgaccccggg gctgaaaaca aaacaataac ccgcgcgcag
     1501 tgcgaggagc ccgggtgtga agcaatcagc aggtaatatt aatgacaaga ccagcaggtt
     1561 ctttagacaa acgaacaata tcagcaaata cgacagttcc aggagaggta aaaagttttt
     1621 tacttgcaca attcttaaaa aaatggggcg acatacctgg aaggtttcac ctcagacaga
     1681 gagcccggaa caaaaacagt gtgccggagc aaagggccgg taacttaccg gtaattcaaa
     1741 acagtcagaa taatctttat gcctcatata ctcaggatgt tctcttatca gcggtcatgg
     1801 ctgaatataa tcccaggtga aaatgccttc tgatcgttaa gcaggttatt gtgaacagga
     1861 aataaagaga agaatacgtc catgccgctc gatgcagcaa cacgttgagt ggcacacatg
     1921 ctggtgatat tgatgatgtt ctgtcatggg gagcgtagtc cgtacccgta cagcatgaac
     1981 agggcggtgt gttttgataa tccggcactt gcggataaaa cctgcacaga gctgtttcat
     2041 ctggtggata tggcggtgat ggacggcgat gaaatcatgc agtaccgcaa acagtcgttc
     2101 agtcctttat gtaactcgtt atggtaacaa tccgttggta atgggagaga tgaactatcg
     2161 caagcgtaaa ctgaccgctc tattcgatca cggccaaccc gtgatcgatt cagatgccat
     2221 tttgtacgtt gtgcaacttc tgtgatgatg gtctggcgga ggcggataat aatgccgcgg
     2281 aaagagcgct tcgtgcagtc tgtctcggaa a
//
LOCUS       DAZFRG010000419         2279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFRG010000419 DAZFRG010000000
VERSION     DAZFRG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.421"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..833
                     /gene="nirC"
                     /locus_tag="ACHKUC_004956"
     CDS             27..833
                     /gene="nirC"
                     /locus_tag="ACHKUC_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO6621017.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            852..2225
                     /gene="cysG"
                     /locus_tag="ACHKUC_004957"
     CDS             852..2225
                     /gene="cysG"
                     /locus_tag="ACHKUC_004957"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO6621018.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          489 a          568 c          653 g          569 t
ORIGIN      
        1 gtttttattt tttaaaggat aatcaaatgt ttacagacac tattactaag tgtgcggcta
       61 acgctgcgcg cattgcacgc ctgtcggcaa ataacccgct cggcttttgg gtcagctccg
      121 cgatggcggg cgcgtatgtg ggtcttggga tcatcctgat tttcacgctc ggtaatttgc
      181 tcgacccgtc cgtacgtcct ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg
      241 tgattatcgc cggttctgaa ttgttcaccg gacacaccat gttcctcacc tttggggtga
      301 aagcgggcac catcagccac gggcaaatgt gggcaatcct gccgcaaacc tggctgggta
      361 acctggtcgg ttccgtcttc gttgccatgc tttatagctg gggcggcggt agcctgctgc
      421 cggtagatac cagcatcgtt cactccgttg cgctggctaa aaccactgca ccggcaatgg
      481 tactgttctt caaaggtgca ttgtgtaact ggctggtttg cctggcaatc tggatggcgc
      541 tgcgcactga aggggcggcg aaatttatcg ctatctggtg gtgtttgctg gcatttatcg
      601 cgtccggcta cgagcactcc atcgctaaca tgacgctgtt cgcactctcc tggttcggca
      661 accacagcga agcctacacg ttggcgggca ttggtcataa cctgctgtgg gtgacgctgg
      721 gtaatacttt atcgggtgcc gtattcatgg gattgggtta ttggtatgct acgccgaaag
      781 cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac ggctgccggt taattactaa
      841 ggggttttta cgtggatcat ttgcctatat tttgccaatt acgcgatcgc gactgtctga
      901 ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt gctgttagac gcaggcgctc
      961 gcttaacggt gaatgcatta gcgtttattc cacagttcac cgcatgggca gatgcaggca
     1021 tgttaaccct cgtcgaaggg ccatttgatg aaagccttct cgacacctgc tggctggcga
     1081 ttgcagcgac agatgatgac gcgcttaacc agcgcgtcag cgaagccgct gaagctcgtc
     1141 gcatcttctg taacgtggtc gatgcgccga aagccgccag ctttattatg ccgtcgatta
     1201 ttgaccgctc accgctcatg gtagcggtat cctctggcgg cacctctccg gttctggcgc
     1261 gcctgttgcg cgaaaaactc gaatcactgc tgccgttaca tctgggccag gtagcgaaat
     1321 acgccgggca attacgcggg cgagtaaaac agcagttcgc cacgatgggt gagcgtcgcc
     1381 gtttctggga gaaattgttc gttaacgacc gcctggcgca gtcgctggcc aacaacgatc
     1441 agaaagccat tactgaaacc accgaacagt taatcaatga accgctcgac catcgcggtg
     1501 aagtggtgct ggttggtgca ggtccgggcg atgccgggct gctgacactg aaaggtctgc
     1561 aacaaattca gcaggcagat gtggtggtct acgaccgtct ggtttctgac gatattatga
     1621 atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa acgcgcggga taccactgcg
     1681 taccgcagga agagattaac cagatcctgc tgcgggaagc gcaaaaaggt aaacgcgtgg
     1741 tgcgtctgaa aggcggcgat ccgtttattt tcgggcgtgg cggagaagag ctggaaacac
     1801 tgtgcaacgc gggtattcca ttctcggtgg ttccgggtat taccgcagct tctggttgct
     1861 ctgcctattc gggtattccg ctcacgcatc gcgattatgc ccagagcgta cgcttaatta
     1921 ctggacactt aaaaaccggg ggcgagctgg actgggaaaa cctggcggca gaaaaacaga
     1981 cgctggtgtt ttatatgggg ctgaatcagg ctgcaactat tcagcaaaag ctgattgaat
     2041 acggtatgcc tggtgaaatg ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc
     2101 gcgtgattga cggtacgctc acgcagctgg gcgaactggc gcagcaaatg aacagtccat
     2161 cgctaattat tattggtcgg gttgttggcc tgcgcgataa attgaactgg ttctctaacc
     2221 attaatttaa ccagggccag agaagtctgg cctttttcgt ttacaactct ctttatata
//
LOCUS       DAZFRG010000420         2252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFRG010000420 DAZFRG010000000
VERSION     DAZFRG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.422"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..480)
                     /locus_tag="ACHKUC_004958"
     CDS             complement(<1..480)
                     /locus_tag="ACHKUC_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6621019.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     "
     gene            complement(532..2214)
                     /gene="ydeN"
                     /locus_tag="ACHKUC_004959"
     CDS             complement(532..2214)
                     /gene="ydeN"
                     /locus_tag="ACHKUC_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6621020.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          588 a          471 c          502 g          691 t
ORIGIN      
        1 aatgacggtt aacgtattaa actctatttg atatgatttc agacgctcga tggctgctat
       61 cacttttgca aaagtaccgt tacctgaatt actgcgtctg tagcggtcat gtagttcttg
      121 gggtccatcg atcgagatac caaccagaaa ctcatgttct ttgagaaagg cacaccattc
      181 attattcaat aaaatgccat tcgtttgtaa tgcattaaaa attcgttttt ggcctgcata
      241 gcgttgttga tagtgaataa ctttacggaa aaaatccagg ccagccagag tgggttcacc
      301 gccttgccag gtaaaataga cctgattgcc agacgctgcg atatattgtt tgatgaactc
      361 tttcagagtg ctgtcatcca tccatttttc atgagtaaac tgcgactctt tttcaaggta
      421 aaaacagtaa tcacatttga gattacattg aaaactggag ggcttggctg taacgtgcat
      481 cgctatctcg ctcaataagg cggcggaaaa atccgccgca tgaaggttta gttatttcgc
      541 ttcgcttaat gctttcttga tattgttaaa cttctcctga tttacctcgc taagcggtgg
      601 ctggctgtta tcgataaact ctcttaccac gccttgcatc tctttaacga cctgcggatt
      661 ggcggcggca aggttatctt tttgctgtag atccgtcagt ttgtatagac ctaactgatt
      721 attttctact gtatagacga gcgaataatc gttatttctc accgtataag agaattggct
      781 taagtcctcg gtgttggggt tatgcgggta atcgtctgac tgatggcgga caaatttgtg
      841 gtaattatcc cagaatggaa tgttttcctc atcaaaccag tgagaataag atgttatcca
      901 ggtcagattt ttatgtggct cgccttgttt cttattttgc aaccagggca gcaaggaaac
      961 gccatccagc ttaaggtctt ttggaatgct gatatcggct gcatcaagag ctgtcgggta
     1021 gaaatccatt gcggaaatca gcttgtcata attaccgggt tgaagttttc ctttccacca
     1081 cataaacatt ggggtgtgag taccgccagg ataggtctga ctcttatagc ctttttgcgc
     1141 cccgttcagc ggcagaggac catcgataac tgcaccatta tcggaggtaa agagaataat
     1201 tgtattgtca tactgtccgt ttttcttcag ttgttcgaga atgcgtttta caccctgatc
     1261 aacagaataa acggaagcgt agtaattatc tgctgtttga ctgccggtat taaattgctt
     1321 ctgatattgc tccggagcag gattatcatt tggcagatgc ggagcattat aagccaggta
     1381 aagcataaaa ggctggtcaa gtgttttggc acgatcaaca acgccaattg cctcatcggt
     1441 taactgatcg ctgatataac cttttgcggg gacgcgttca cggtttttga acagtgaagg
     1501 ggagttgtaa tatgccgttc ccgcagcatg gaatcccata aagtaatcaa agccacggtt
     1561 ttgcggttgc cattcttccg cagaaaaggt agtgaagttg tcatgatagt cacgcgtttg
     1621 tttatcttcc ggcactggca cattactgat ttttgacaag tgccatttac ctactgctgc
     1681 agtgtaataa ccatgattct ggaataattc aggcaagaaa gtttctgtta gcggaatacc
     1741 atcctgagca tcagtattgg aatagacacc aaagcgggcg ggcgctcgac cggtcattat
     1801 tgcggcgcgg gacgggccgg aaacaccgtg cgccacatag ccgttagtaa aacgtacgcc
     1861 ttcatccatt aatgaaagga gcgtcggcgt tgatttttgc gcagcttcaa tggctttatc
     1921 tatccctatt ttgtaggtat cgacaacttc acgattttcc attgtttttg ggtcaaaaga
     1981 tcccttatca aaaggaagtt gtccataacc aagatcatcc atggtcagta cgataatatt
     2041 tggctttcct ttggtactgt attcagtcgg cgtaaagtct gagaaagcaa cgtttgtttt
     2101 ggttgctttc agctttacat catctgccgc atgagcagca aatgcagcca taccagatgc
     2161 cagtatcaaa gatatcgagg tacttacgac acttttcttt aatgcagact tcataaatgt
     2221 tcctcttctt attgtactta attccaaagt aa
//
LOCUS       DAZFRG010000421         2216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFRG010000421 DAZFRG010000000
VERSION     DAZFRG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.423"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2052
                     /locus_tag="ACHKUC_004960"
     CDS             <1..2052
                     /locus_tag="ACHKUC_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6621021.1"
                     /translation="DNGINAFLMNYQANAQINQYRNKKNREVSSYWARIEPGMNIGSW
                     RIRNLTTFTKENGNSEKRESVYTYAERGLTSIKSNLLIGESYTNSDIFDSISFRGIML
                     HSDESMVPYSKYAFAPVIRGIAQSQALIEVRQNGYLIHTVSVAPGAFEISDLPVTGSG
                     GDLQVSVIETNGKNQSFTVPYTTPVIALRENYLKYSLVGGMYRSAYSGVDNTALIQMT
                     AMYGMPWNLTTFVGFQGSEHYNSVATGVGLSMGDMGAVSLDGIYARGQKEKQNKEDGY
                     SWRVRYSKVFDITGTNFIAASHQYSSDGYQTLSDVLDTYGHSNYSYGGYANRSMRNSL
                     TISQSMGEWGTFSFWGVRDEYRGNRSPQNSINALYSNSMEWGTLSLNWSQNKITDSSR
                     RVKDKKENIFSFWVSIPLYRLLGNTSNNINATTQIQKYDNQKMQYEFGMNGRAFNRQL
                     YWDISQRLAPGNENYNDASRLNLEWYGTYGQIRGGYGYSDSLRQMNAGISGTAIVHSN
                     GVTFGQKQGGTIALVEAQGVDGAEVIGWPGVKTDFRGYTALGHLTPYQENTVSLNPAS
                     FPEYAEVLQTDTKVIPTKGAVVSARFKTSIGKKALFKLTRHDGKKVPFGAVVSSATAD
                     DNKRVVGIVNESGEVYMSGLSEKGQLDVKWSSHGSCKAVYKLSDNKSIVNIYNASLTC
                     I"
BASE COUNT          687 a          358 c          523 g          648 t
ORIGIN      
        1 gataatggaa ttaatgcatt tctgatgaat taccaagcta atgcacaaat aaaccaatat
       61 cgtaacaaaa aaaatcgaga agtcagctct tattgggcca ggatcgagcc tggtatgaat
      121 attggttctt ggcgcataag aaacttgaca acttttacta aagaaaatgg taattctgaa
      181 aaaagagaaa gcgtttacac atatgctgaa cgtggtctca catccataaa aagcaatctg
      241 ctgataggtg agtcctatac taactctgat atatttgaca gcatatcttt cagggggata
      301 atgctccatt cagatgagag tatggttcct tatagcaagt atgcttttgc acctgtaatc
      361 agaggtattg cacagtctca ggctttgatt gaggttcggc agaatggtta tctcattcat
      421 actgttagtg tggctccggg ggcttttgaa atctctgacc tacctgtcac aggctcaggt
      481 ggtgatttac aagtttccgt tatagaaaca aatggtaaaa accagtcttt caccgtccct
      541 tacactacac ctgtgattgc tttgcgggaa aactatctga aatatagctt ggttggcggt
      601 atgtatcgtt cggcttattc tggtgtggac aatactgctt tgattcagat gacagcaatg
      661 tatggtatgc catggaatct gacaacattt gtaggattcc aaggctctga acattacaat
      721 tctgtcgcaa caggtgtcgg attgtctatg ggggatatgg gggccgtatc gcttgatgga
      781 atatatgctc gaggacagaa agaaaaacag aataaagaag atggatattc atggagagta
      841 agatacagta aggtatttga tataacagga actaatttta ttgctgctag tcatcagtac
      901 tcttctgatg gttatcagac tctttctgat gtacttgata cttatggcca tagcaattac
      961 tcctacggag gttatgctaa tcgctctatg cggaatagtc tgacaataag tcagtcaatg
     1021 ggggaatggg ggacctttag tttttggggg gttagggatg aatatagagg aaatagatcg
     1081 ccacagaatt ctataaatgc gttatatagc aatagtatgg aatggggaac attgtctctt
     1141 aactggagtc agaataagat aactgactct tcgagaagag tgaaagataa gaaagaaaat
     1201 atcttcagct tttgggtgag tattcctcta tacagattac ttggaaatac gtctaacaat
     1261 ataaatgcaa caacacagat acagaaatat gataaccaaa aaatgcagta tgaatttggt
     1321 atgaatggtc gtgcattcaa caggcagttg tactgggaca ttagtcaacg attggcacca
     1381 ggaaacgaaa attataatga tgccagcaga ttaaaccttg agtggtatgg tacttatggt
     1441 cagattcgag gcggatacgg ttacagcgat tctctcagac agatgaacgc aggaatatca
     1501 ggaaccgcca tcgttcacag taatggagtt acgtttggac agaaacaggg tgggactatt
     1561 gcccttgttg aggcgcaggg ggtagacgga gcggaagtta ttggatggcc tggtgttaaa
     1621 acagacttca gaggttatac agcattgggc catcttacac catatcagga aaacactgtt
     1681 tcattgaacc ccgcttcttt ccctgaatat gcagaagtat tgcagacaga tacaaaagtt
     1741 atacctacaa aaggtgcggt ggtttcggca aggtttaaaa catctattgg taaaaaggcg
     1801 ttgtttaagt taacaagaca cgatgggaaa aaagttcctt ttggtgctgt ggtttcatca
     1861 gcaacggcag atgataataa gcgcgttgta gggattgtaa atgagtcagg tgaagtgtat
     1921 atgtcaggcc tgtctgaaaa aggacaactg gatgtgaaat ggagcagtca tggaagctgt
     1981 aaggcagttt ataagctttc tgataataaa agcattgtta atatatataa tgcatccttg
     2041 acttgtatat aaaacggaaa gtattatatg gtacttaaag gcttactttc tttttttctt
     2101 gttttttctt caacggttat cgctgccgaa attaatcttg agacaagggc attttcaggt
     2161 agttatttat atgatggtga tattatcgct accggaagtg tattttgcga tgatgg
//
LOCUS       DAZFRG010000422         2207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFRG010000422 DAZFRG010000000
VERSION     DAZFRG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.424"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..1527
                     /locus_tag="ACHKUC_004961"
     CDS             10..1527
                     /locus_tag="ACHKUC_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6621022.1"
                     /translation="MDIIIVPRDQRIPASGISTAYLHVDHWNDFSFITMFYMYLFDQK
                     GERHEIGNVKIGFQGQTTQQSTYSTLGDRFKILPEGYFSVGQDVDYYQRISNLPESVK
                     VSLLEALKDIAYTPELIDFVKNEAVFKTSLLRYVSLSVIKGQFARVLEGKSPLTNFEF
                     KFIRPAQDKISDIELSFKVKVGEKPSTNIHAIIGRNGVGKTTILNGMIEAVTSKGTSN
                     AKFYDLEGWREDPIDNDYFSSLVSVSFSAFDPFEPPSEQPDPSKGTCYFYIGLKKKGE
                     SLKGLNDIHEDFLEALTLCFSQHPKRERWLKAIDTLESDENFASMELKNLAEYNDEML
                     VSNARKCIKRMSSGHAVVLLTLTRLVATVEEKTLVLIDEPESHLHPPLLSAFIRALSD
                     LLLDRNGLSIIATHSPVVLQEVPKRCVWKINRSRLSTESRRPDIETFGENVGILTREV
                     FGLEVVKSGFHDLLVQSVKSGGTYEEIVDEYRNQLGVEARALLKALVTHRDRSVK"
     gene            1527..>2207
                     /locus_tag="ACHKUC_004962"
     CDS             1527..>2207
                     /locus_tag="ACHKUC_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6621023.1"
                     /translation="MLKLKQPAYTYADMLTSCREGITGNPGLLLKVNEAITDLQTLAA
                     SYSASASTGELYTIQPLKNKDKEDPIVVGRLKKSDFVKLYGNYVVGKKKPARAIYDSL
                     ITAANEKCPYCGGIGRPRNLDHYLPKAYYPQFSILPVNLVPACRDCNMDGKGEVFARN
                     EAEQIIHPYLDDSRYYDEQWISARYLAGKNDEPGVIEYFVSPPADWDEAHKQRVEKHF
                     NDFDLSLRY"
BASE COUNT          671 a          381 c          494 g          661 t
ORIGIN      
        1 ggaaattata tggatatcat tattgtaccc cgagaccaac gtattcctgc ctcaggcatt
       61 agtacggctt acttgcatgt ggatcattgg aatgactttt cattcattac aatgttttac
      121 atgtatctgt ttgatcagaa aggtgaacgc catgaaattg ggaatgtcaa gattggtttt
      181 caaggacaaa caactcaaca atcaacttac tccacattag gggacaggtt taaaattctc
      241 ccagaaggat atttttccgt tggtcaggat gtcgactatt atcagaggat ttcaaattta
      301 ccagaatccg tcaaagtttc gttacttgag gcgctcaaag atattgctta tacgccagaa
      361 cttatagatt tcgttaagaa tgaagcggtc tttaaaacgt ctttgcttcg gtatgttagc
      421 ctgtctgtta ttaaaggaca gtttgcgcga gtgcttgagg gaaagagtcc actgactaat
      481 tttgagttta agttcattcg ccctgcacaa gacaaaattt ccgatattga attatcattt
      541 aaggtaaaag taggagaaaa accaagtacc aatattcatg cgatcatagg tcgtaatggt
      601 gtggggaaaa cgacgattct aaatggaatg attgaagctg ttaccagtaa aggtacgtcg
      661 aacgcaaaat tttatgatct ggaggggtgg agagaagacc cgattgataa tgattacttt
      721 agtagtttag tctctgtgtc atttagcgca ttcgatccat tcgagccacc aagtgaacaa
      781 cctgatccat caaagggaac ttgctacttc tatataggtt tgaaaaaaaa aggagagtcg
      841 ctgaaagggc taaatgatat tcatgaagac tttctagaag ctttgacgct atgctttagc
      901 cagcatccta agcgtgaaag atggctgaag gccattgata ctttggagtc tgatgaaaat
      961 tttgcaagca tggagctgaa aaatctagcg gaatacaatg atgagatgct cgttagtaat
     1021 gcacggaaat gtataaaaag gatgagttcc ggtcatgcgg ttgtgttatt aacactcaca
     1081 cgtttagtcg caacggtaga agaaaaaacg ttagtgctaa ttgatgaacc agagagccat
     1141 cttcatcctc cgttgctgtc tgcttttatt cgtgcgctgt ctgatttgct acttgacaga
     1201 aatggtcttt ctattattgc tactcactca cctgtggtac ttcaagaggt ccctaaacga
     1261 tgtgtttgga aaattaatcg ttctagatta tctaccgaat ctcgcaggcc agacattgaa
     1321 acatttggtg agaatgtagg aatcttaaca agagaggtgt ttggtcttga agttgtcaaa
     1381 tctggttttc acgatttatt agtccaatca gttaagtcag gtggaacata tgaggagata
     1441 gttgatgagt atcgtaacca gcttggtgtt gaagctcgag ctctattaaa agcgcttgtc
     1501 acacacagag atcggagtgt taagtaatgt tgaaacttaa acaacctgct tacacatatg
     1561 ccgatatgct gactagttgc cgcgaaggca taaccggtaa tccgggcttg ttgctgaaag
     1621 taaatgaagc aataactgat ttgcaaacac tggcagcttc atatagcgca tcggcatcaa
     1681 ctggtgagct ttatacaata caaccattga aaaacaaaga taaggaagac cctattgttg
     1741 tcggtcggtt aaagaaatct gactttgtta agctttatgg caattatgtt gttgggaaga
     1801 aaaagcctgc tcgagctatt tatgattccc tgattactgc tgctaatgaa aaatgtccat
     1861 attgtggtgg aattgggcgc cctcgaaatt tagatcatta tttgcctaaa gcttactatc
     1921 ctcagttttc tatattgcca gttaatctgg tgcctgcttg tcgagactgt aatatggatg
     1981 gcaaagggga agtttttgca agaaatgaag cagaacagat catacatccc tatttagacg
     2041 atagccgtta ttatgatgaa cagtggatat cggctagata cttggctggc aaaaatgatg
     2101 agccaggagt tattgagtat tttgtttctc caccggctga ttgggatgaa gcacacaaac
     2161 aaagagtgga aaaacatttt aacgattttg atttgtcttt aagatat
//
LOCUS       DAZFRG010000423         2204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFRG010000423 DAZFRG010000000
VERSION     DAZFRG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.425"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            688..1356
                     /locus_tag="ACHKUC_004963"
     CDS             688..1356
                     /locus_tag="ACHKUC_004963"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFO6621024.1"
                     /translation="MSHPIINVGRYHGGSDDWRTPYRLFHNLHREFNFSLDGAATEHD
                     ALLPRFTDDIHNQSWVGERVFVNPPFSMAEKFLLKAPEADVCVCLVPLRSKTTYWLRC
                     VYTNPFLHEIRTLHRAVKYLPPAGKRGVVVRSPFPSCVLIYRNTPRKPKVEVTQTIYC
                     GDTMLLLAVVNRGGLRGRPHMHNAQTLDRLIKMYDQGQPIKSIAESLRMPLSTTYRIV
                     QRLG"
     gene            complement(1823..1999)
                     /locus_tag="ACHKUC_004964"
     CDS             complement(1823..1999)
                     /locus_tag="ACHKUC_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621025.1"
                     /translation="MVNLPPELGSPDVWAGGGVGLIAGLLLRYVLVRVLVVFKLFGRT
                     WTLDLKDNDHTEPR"
BASE COUNT          661 a          468 c          476 g          599 t
ORIGIN      
        1 gtcgattttt aacgtggcta cgacaatcca cccataccgc accaaacctt accccaccga
       61 accgcaccct acacgacctt acctcgccgg acactgccat agagagtgtg cttggttgct
      121 ttggaagtaa acctagtaca gtaagccatt atacatatcg agatagtaac gtcaagaaac
      181 ttttaacagg aggggttcct atggtcacat attccctctc ttcccactaa gtaagtctta
      241 gcagcaccta agcaacaatt gactaacagg ttcggtagag tctatctcat aggtcaactt
      301 cataaactca gagttaagtt accttgaaag attctaccaa gaaactacac aggtgttatt
      361 tagtctgact gtgaagggat atttaacggg aggagagata cgatataacc cgaagggaga
      421 gtatcatctt aacaattatc ttaagaataa aactgaaaag ttcttaacag atgactacct
      481 agaaagaagg gaacgataag accagaggta gtgaccttgt taaacaggct gtttcaaagc
      541 tcttaagagt ctgttaagta agggctatgt gaacaatctt accattttgt caatgctttt
      601 gtctttagca aaaagtgcta aacttaacaa aaagcactaa atgctaaaga gacgattaag
      661 aaatatacta agaggggcgc ttcgcttgtg tcacatccaa taatcaatgt tggtcgttat
      721 catggtggtt ctgatgactg gcgtacaccg tatcgtttat ttcataatct gcatcgtgag
      781 tttaacttca gtctagatgg cgctgcaact gagcatgacg cattgttgcc acggttcacc
      841 gatgatattc acaatcaatc atgggttggt gaacgggtct tcgttaaccc gccgtttagc
      901 atggctgaaa agttcctctt aaaagcccct gaagctgatg tgtgtgtatg tctggttccg
      961 ctcaggtcaa agacaactta ctggcttcgg tgtgtatata caaacccctt cctgcatgag
     1021 attcgaacgt tgcaccgagc agtcaaatac cttccaccag caggaaagag aggtgttgta
     1081 gtgcgctcac cgtttccaag ttgcgttttg atatacagga acacaccccg caaaccgaaa
     1141 gttgaagtta cccagacaat ctattgtggt gacacaatgc ttttacttgc cgtggttaac
     1201 aggggtggtt tacgtggaag acctcatatg cacaatgcac agacgcttga caggttgatt
     1261 aaaatgtatg accaagggca acccatcaag agcatcgcag aatcgctaag aatgcctcta
     1321 agcaccactt acaggattgt tcaaaggttg ggctgaagtt ctcatataga gcactgatta
     1381 cgagaatgtt aaagatgcga tttccccttt ctggggttat ttgcttttta ctggaagggt
     1441 tattttacag aggtttttca aaccccctca ataatagaaa cgagcaacaa acgaagagcg
     1501 agaaataaaa atattgagac tggtgaacgg gggtctgtaa agattgttta agaagtcggt
     1561 taagaaggtg gctctccctg tctggggtta tcgccttgtg tcttagtgag tcttgaaagt
     1621 tctctgtgaa gagtcttgtc agtcctttat attggctaat tacaactcat tgaaaaaata
     1681 agcattttta gaaattactg tcagaggggt gaatacttga tagatacagc tttccggttc
     1741 tgtcaagcta tttattaatg gatagcacaa aatgctaaag atacccctat accatcacgt
     1801 acaggggggt tttgaaggga gtttaacggg gttctgtgtg gtcgttatcc ttaaggtcaa
     1861 gcgtccatgt acgaccgaac agtttaaaga caacaagcac tctgacgagg acgtagcgaa
     1921 gtagcagacc tgcaatcagc ccaacacctc caccagccca tacatcaggt gaccccagct
     1981 caggagggag atttaccatt agttttcctt gtaagacgct ctcaatcgca ttctgacaca
     2041 aaaaagtacc tgaccttata tgttgacctg aagaggccat ttaactcgtt acagagcgat
     2101 tgaagaggtc tgtgtgcaat gcaataacca tgcggccatt ctcatagcca cgcctcatat
     2161 tttactctcc cataaaccca ttataccaca gatgttaata aaat
//
LOCUS       DAZFRG010000424         2168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFRG010000424 DAZFRG010000000
VERSION     DAZFRG010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.426"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2047
                     /locus_tag="ACHKUC_004965"
     CDS             <1..2047
                     /locus_tag="ACHKUC_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6621026.1"
                     /translation="KSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
BASE COUNT          672 a          413 c          526 g          557 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacagctg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggtag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctggcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaaag attgttcagt attcagacac ctaaaaagaa atttaatgcc
     2161 aataaatt
//
LOCUS       DAZFRG010000425         2140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFRG010000425 DAZFRG010000000
VERSION     DAZFRG010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.427"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(587..685)
                     /locus_tag="ACHKUC_004966"
                     /pseudo
     CDS             complement(587..685)
                     /locus_tag="ACHKUC_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
     gene            complement(775..1143)
                     /locus_tag="ACHKUC_004967"
     CDS             complement(775..1143)
                     /locus_tag="ACHKUC_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6621027.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(1306..1527)
                     /locus_tag="ACHKUC_004968"
     CDS             complement(1306..1527)
                     /locus_tag="ACHKUC_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6621028.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(1590..2066)
                     /locus_tag="ACHKUC_004969"
     CDS             complement(1590..2066)
                     /locus_tag="ACHKUC_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6621029.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            complement(2082..>2140)
                     /locus_tag="ACHKUC_004970"
     CDS             complement(2082..>2140)
                     /locus_tag="ACHKUC_004970"
                     /inference="COORDINATES: protein motif:HMM:NF015205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6621030.1"
                     /translation="RDYALQHPECSAIMRIID"
BASE COUNT          535 a          513 c          586 g          506 t
ORIGIN      
        1 agcagaggaa aacatctatt attaccatgt aattcatgtt gttatgtcgc aacaatagta
       61 ctgcacgggg aattattacg ggcaaactta ggcgaaagga agtactgaag tgacagaata
      121 aatcacactt tttgtacata tgtacttttc tattgtcttt tgttgtggaa acaaatggtg
      181 tttgtcatgc cgatgctaat agacagatag attcgagggc caacagcccc atcatcatga
      241 tggagcaaca gaggtgagtc taacgtttcc gcacttctta tcgatcggca ctaacgatca
      301 atatcgataa actgatctat caaaacgatt atctttcttg caacctgcct agttatcctt
      361 ctcttacatt gctcgcagaa aagccctttt attacggtta gttatctagc tgaggcttgc
      421 tacgcccatt ttccatgtac cccaaatcac gaggtcacaa aggcgcggaa agatgaaggg
      481 atgtgcacgt tgcaacaaaa aaaagaagcg tccttgaaca gaggctctca ctcgataaag
      541 aattctgcga cgagtgctta ttcgatatcg atgaggaaat cagggaatgc tggtccagta
      601 accgggtgag cagtgtctgc cagatggtga ctggagacgg gcagcacgat acctcccgta
      661 catgcgtgtc gggtaatgtt ttcatactga agattttcct gatatgcaga gataaaaaat
      721 gggacagtgg cgtggtgaaa acaccaggcc ggagcagaag gttattctgg tggtttactt
      781 tttcgtttca ggtgtcggat aaacagccag ataaacgtaa ccacaggagc cgagggtgtc
      841 ggcttcacag gtcagcccct tcgcatacag tgtcacggta tgctgatggc ggggattcag
      901 ttcaccactg gtgagcatta atttcagttg tttcatcagc agcggaaagg cctggtccag
      961 agggtacgca tccgcatctc tgaaccggcc tctgataccg gcacggtcgg caaggtaatg
     1021 caaccggtta ccctcctgca ccagacgggc gccgaaacag ggcgtcactg tgcagggtag
     1081 tccccaccag ggatggtcgt cattgtcatc gggatgcgtt gtcccggaaa gtgtgtctga
     1141 cacgataaaa tcctcacaga aaatcggtga atgatagttt aacgatgacg ggtcagccgg
     1201 cgcgcagacc ggcttcggta aaggtgtatt cgttgatgag caggacctca tccacagcgg
     1261 cgtctgagct ctgccagtcg tactcgtttt ccgggtgacg gaacatcagt tcagggtgac
     1321 gctcatcagc cggacatacg gaccaaaact gtccttacgg cgttctgcaa acacggccaa
     1381 cacaccggga atatcctgca cttcacgacc ggtatacgct tcagcactgc cgtgccagcg
     1441 gtatttgccg gtacagaacg gaaaaagacg ggatgccgga tgctgacggt gaatacgcat
     1501 ggcttcgcca cgggtgatga ttttcatagt gggatacctc tgaagacaga agataaaagt
     1561 gaaaacaggt gtgatgtggt tgtgacgggt taaagcagac catattcggc aaaggagaaa
     1621 acctggctgc caccgactat cagatggtcc ggcacccgga tatccaccag gcccagtgcc
     1681 tgtaccagac gttccgtgat aaggcggtct gccttgctgg gtgtgacttc accggacggg
     1741 tgattgtgtg ccagcaccac agcagcggca ttgtgataca gggcgcgttt aatcacttcc
     1801 cggggatgga cttccgtgcg gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc
     1861 tgattctggt tgttcagata cagcacccgg aactcttcac gctccagtcc cgccatgttc
     1921 agaatcagcc attctcgtgc cgcacgggtg gaggtgaagg ccacgccggg ttcatgaaga
     1981 tggcggtcca gggttttcag ggcccgttga atgaggctgc gctcgccggg cgtcatctct
     2041 ccgggcagaa aggaaagctg ttgcattgta cttctctcca ttcagtcgat gatgcgcata
     2101 atggcgctgc attccggatg ctgcagggcg taatcccgca
//
LOCUS       DAZFRG010000426         2137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFRG010000426 DAZFRG010000000
VERSION     DAZFRG010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.428"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..395
                     /locus_tag="ACHKUC_004971"
     CDS             <1..395
                     /locus_tag="ACHKUC_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621031.1"
                     /translation="GQAPEMVKKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFV
                     TSLAGFSPKQTPAINKDSVLQAARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNP
                     EDRAYDKVTVTVGADGNATGASVEFIGK"
     gene            401..634
                     /locus_tag="ACHKUC_004972"
     CDS             401..634
                     /locus_tag="ACHKUC_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6621032.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIH
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            724..1542
                     /locus_tag="ACHKUC_004973"
     CDS             724..1542
                     /locus_tag="ACHKUC_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6621033.1"
                     /translation="MQLASRFGHVNQIRRDRPLTREELMQVVPSVFGEDKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMRSLVLPPPARQALAQAALTYRYGDEHHPITTADILTPRRREDYGQDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMTETLLENMR"
     gene            1633..2118
                     /locus_tag="ACHKUC_004974"
     CDS             1633..2118
                     /locus_tag="ACHKUC_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6621034.1"
                     /translation="MKTVSQNTCTLSASVVTASENDQPQLVATLVPHEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRII
                     D"
BASE COUNT          522 a          568 c          561 g          486 t
ORIGIN      
        1 tcggacaggc gccggaaatg gtcaaaaaag ccgttcactc catagcagag cgtgatgtgt
       61 ccgttttaca ggcatttggt gtacgaatgg aattcacccg cggtgatatg ctgaaggtct
      121 gtccgaaatt tgtcacatca cttgctggtt tttccccgaa acagacaccc gccattaata
      181 aagattccgt cctgcaggct gcccggcagg catacgcccg ggaatatgac gaggaaacaa
      241 cagaaaccgc tgattttggc tcttacgaag taaaaggcaa taaggttgag tttgaagtgt
      301 tcaatcctga agaccgtgcg tacgacaaag tgaccgtcac ggttggtgct gacggtaatg
      361 ccaccggtgc cagcgttgaa tttatcggaa aatagccggt atgtcggact gtcaccctgt
      421 tttattgccc gaaggccctt tctcacgcga acaggcgatg gctgtcacaa cagcttaccg
      481 caatgtgctt attgaagatg atcagggaac gcatttccgg ctggttatcc acaatgccga
      541 agggcagcta cgctggcggt gctggaattt tgaacctgat gccggaaaac agctaaatcc
      601 atatctcgcc agtgagggaa ttctcaggca ataaacgtct tcatttcatc catcaggccg
      661 cgtcttctcc gggagacgcg gccttttcat ttataccgct aactcattca taaggagcaa
      721 agtatgcaat tagccagtcg ttttggtcat gtaaatcaga tacgccgtga tcgtccctta
      781 actcgtgaag aactgatgca ggtcgtcccc agcgtattcg gggaagataa gcacacatcc
      841 agaagcgaga actatacctg gatccccacc atcacagtgc tggaaagcct gcagcgggaa
      901 ggttttcagc cattcttcgc ctgccagacc cgtgtgcgcg acccgggccg ccggggatac
      961 accaaacaca tgctgcgtct gcgccgtgac ggggagataa acggacaaca tgtccctgaa
     1021 atcatcctgc tgaactccca tgacggtacc tccagctacc agatgctgcc gggttacttc
     1081 aggttcgtct gccagaacgg atgcgtctgt ggtcagtctc tgggggaagt gcgtgttcca
     1141 caccggggaa atgtagtgga gaaagttatc gaaggggctt acgaggtggt gggggttttt
     1201 gaccggattg aagaaaagcg tgacgccatg cggtcgctgg tcttaccgcc accggcacgc
     1261 caggcactgg cgcaggctgc tctgacttac cgttatggtg acgaacatca cccaatcacc
     1321 actgctgaca ttctgacacc acgacgccgg gaggattacg ggcaggacct gtggagtgct
     1381 tatcagacca tccaagagaa tatgctgaaa ggcgggattt ccggtcgcag cgctaaagga
     1441 aaacgtatcc acacccgtgc cattcacagc atcgacacgg acattaagct caatcgcgca
     1501 ttgtgggtaa tgacagaaac gctgctggag aacatgcgct gatgcagcac atccctgaag
     1561 acgtgattca ggctccccta ccccctgctc cccgaaaacc ctgcatttat ttgttttata
     1621 aggatttcac tcatgaaaac tgtttctcag aacacctgta cactttctgc ttccgtagtc
     1681 acagcttcag agaatgacca gcctcaactg gttgccactc tcgttcctca tgaacagcgc
     1741 atcagcttct ggccgcagca ttttggcctc attccacagt gggtgaccct ggaaccccgt
     1801 gtcttcggct ggatggaccg tctgtgcgaa aactactgcg gtggtatctg gaatctgtac
     1861 accctgaaca acggcggggc atttatggca cccgaaccgg atgacgatga tgacgagaca
     1921 tgggtactgt tcaatgccat gaacggtaac cgtgctgaaa tgagcccgga agccgccggc
     1981 attgctgcct gtctgatgac gtacagtcat catgcctgtc gcacggagtg ttatgccatg
     2041 acggttcatt attaccgtct gcgggactac gccctgcagc atccggaatg caacgccatt
     2101 atgcgcatca ttgactgaaa ggggctggaa caatgca
//
LOCUS       DAZFRG010000427         2128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFRG010000427 DAZFRG010000000
VERSION     DAZFRG010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.429"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..1622
                     /gene="lysS"
                     /locus_tag="ACHKUC_004975"
     CDS             105..1622
                     /gene="lysS"
                     /locus_tag="ACHKUC_004975"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6621035.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1741..1914)
                     /gene="ghoT"
                     /locus_tag="ACHKUC_004976"
     CDS             complement(1741..1914)
                     /gene="ghoT"
                     /locus_tag="ACHKUC_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO6621036.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(1942..>2128)
                     /gene="ghoS"
                     /locus_tag="ACHKUC_004977"
     CDS             complement(1942..>2128)
                     /gene="ghoS"
                     /locus_tag="ACHKUC_004977"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6621037.1"
                     /translation="SIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDID
                     FDLSIMTVDDYFRQFYK"
BASE COUNT          596 a          456 c          503 g          573 t
ORIGIN      
        1 tcgataagat aaactgagtt attaatagtc gaggcagata atacagtgta ccgatctgtc
       61 tctttatcta cgctaaattg aaagctggat ttagaggaac caaaatgtct gaacgagaaa
      121 cacggggagc caatgaggct attgatttta acgatgaact gagaaatcgc cgcgaaaaac
      181 tggcggcact acgtcagcaa ggtgtggcgt ttcccaatga ttttcgccgc gaccatacct
      241 ctgaccagtt gcacgaagag tttgatgcga aggataacca ggaactggaa tccttaaaca
      301 ttgaagtctc ggttgctggc cgaatgatga cccgtcgtat tatggggaaa gcctcctttg
      361 taacgttgca ggatgtcggt ggccgtattc aactgtacgt tgcaagagat agcctgccag
      421 aaggtgttta taacgatcag tttaaaaaat gggatctggg tgacattatc ggtgcccgcg
      481 gtacgctgtt taagacgcaa acgggtgagc tttccattca ctgtactgag ctgcgcctgc
      541 tgactaaagc actacgtcct ttaccagata aattccatgg tctgcaggat caggaagtcc
      601 gttatcgtca acgttatctg gacctcatcg ctaacgataa atcccgtcaa acgtttgttg
      661 tccgttcaaa aattctggcc gctatccgtc agttcatggt cgcgcgcggc tttatggaag
      721 tagaaacccc gatgatgcag gtaattccag gtggggcatc tgctcgcccg tttattaccc
      781 atcataacgc tctggattta gatatgtacc tgcgtatcgc gccggagctg tatctgaaac
      841 gtctggttgt aggcggtttt gaacgggtat tcgaaatcaa ccgtaacttc cgtaatgaag
      901 gtatttctgt tcgccataat cctgagttca caatgatgga actctacatg gcatatgcgg
      961 attaccacga tttgattgaa ctgacggagt cactgttccg caccctggca caagaggtac
     1021 tgggtaccac taaagtcact tatggcgagc atgtgtttga tttcggcaaa ccgtttgaaa
     1081 aactcaccat gcgcgaagca atcaaaaaat atcgtccaga aaccgatatg gcggatctgg
     1141 ataattttga tgctgctaaa gcattagctg aatctatcgg tattacggta gagaaaagct
     1201 gggggctggg acgtattgtt acggagatct ttgatgaagt ggcagaagca catctgattc
     1261 agccaacctt tattacggaa tatccggcag aagtgtcccc gctggcgcgc cgtaatgatg
     1321 ttaacccgga aatcaccgac cgttttgaat tcttcatcgg tggtcgtgaa attggtaatg
     1381 gttttagcga attaaacgac gcagaagatc aggctgaacg tttccaggaa caggttaacg
     1441 ctaaagctgc aggtgacgac gaagccatgt tctatgacga agattacgtg actgcgctgg
     1501 aatatggtct gccgccaacc gctggtctgg gtattggtat cgaccgaatg attatgctgt
     1561 ttacaaacag tcatactatt cgcgacgtta ttctcttccc ggcgatgcgc ccacagaaat
     1621 aaatttcact ttaatgaacg aagcagtcag gcgactgctt cgttcgtcat tccgcaaatc
     1681 cagttttgag gagagacgct tcgtttcaag ataaccagcg ggtccactca actacagctc
     1741 ctaaaagaga gaaaaaagta atgccacagg aagactcatt ggccaggtta ttccgaccag
     1801 aaatgcacta agtaaacgaa tatgtgtttt ctcatgtgag ataaaccaga taatgacaaa
     1861 ggatatgttc acaccaatca cataaaaaat taatatttta gagaatagtg ccatttttta
     1921 tgttcttctt atccttcctg gctacttgta aaactgacgg aaatagtcat caactgtcat
     1981 aatgctgagg tcaaaatcaa tatccggtaa gtctaagcat ttgagcctga ttaaatcttc
     2041 gatttcactt gcggctaacg atgtcgttat tgcgaaggag ttttcattca gttgtcgtgg
     2101 tatcccatat tcatcagcca cgatagag
//
LOCUS       DAZFRG010000428         2125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFRG010000428 DAZFRG010000000
VERSION     DAZFRG010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.430"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..779)
                     /locus_tag="ACHKUC_004978"
     CDS             complement(<1..779)
                     /locus_tag="ACHKUC_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFO6621038.1"
                     /translation="MKRSEIRKALEAWFDVERYEAIEKLTLQQFYVEVERRILAYRML
                     LSRNTIPTFNRLMLDDYRNKILSGEIFFSGDTATLGHELARTYAVNPTTRSHAQFYAK
                     TLALTEATPELSGLSQSEFLSEYLKETSLNNLSRITVDIHLEEASTEEIIEHLKVLIP
                     RWKRQLKMKSPAPREYRFGKSTFRKIIEYRLIPMMDLIFWGEDNGIKIPLSLISSLLH
                     EDSDNDRDEGMLKATDYPLAMAFLTDASYLKSLEDYMMENN"
     gene            complement(1058..1147)
                     /locus_tag="ACHKUC_004979"
     tRNA            complement(1058..1147)
                     /locus_tag="ACHKUC_004979"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Ser,seq:cga)
     gene            1241..2038
                     /gene="mtfA"
                     /locus_tag="ACHKUC_004980"
     CDS             1241..2038
                     /gene="mtfA"
                     /locus_tag="ACHKUC_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO6621039.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT          551 a          457 c          487 g          630 t
ORIGIN      
        1 tgattgtttt ccatcatgta atcttcaagc gatttcagat agctcgcatc cgtcagaaaa
       61 gccattgcta acgggtagtc cgttgccttt agcattcctt catcacgatc gttgtcgctg
      121 tcctcgtgaa gcagcgagga aatcagtgaa agcgggattt taatgccgtt atcttctccc
      181 cagaagatca ggtccatcat cgggataaga cgatattcaa taatttttct gaatgtgctt
      241 ttcccaaaac gatactctct tggcgctgga gacttcatct taagctgtct cttccatcgg
      301 gggatcaaca ctttcagatg ttcaatgatc tcttcggttg atgcttcttc cagatgaata
      361 tcgacagtaa tacgtgaaag attattaagg gatgtctctt tcagatactc ggacagaaat
      421 tcagactggc ttaacccaga gagttcaggc gtggcttcgg ttaatgccag cgttttcgca
      481 taaaattgcg cgtggcttcg tgtcgtagga tttactgcgt aggttctggc aagctcatgt
      541 ccaagagtag cggtatcacc actgaaaaag atttcaccgc tgaggatctt gttgcggtaa
      601 tcatccagca ttaatcggtt aaaggtaggt atggtgttcc ggctcaacaa cattctgtaa
      661 gccaagatgc gccgctcaac ctcaacataa aactgctgta aggtaagttt ctctatcgcc
      721 tcatagcgtt ccacatcaaa ccatgcttcc agtgctttcc tgatttcact tcttttcatc
      781 catcctccaa caatccattt cctttgatat atcttcacag gatccgtttg gaacaattga
      841 agttcaactg ttttgtcatc agagacgggg gaatggctaa gttgttgtta agagtgtgaa
      901 ctcgagagca taaaagtgga tgcccgacgc gcggcaaaat ggtaaaaaac agcatcagta
      961 gtaagaaaaa ttgagcgaga gaaaggaatt ttaatgcagg gatttatgca tgacaaaatc
     1021 aaaaatgctt tgtaagtaat tgattttgtt gaatgattgg cggagagagg gggatttgaa
     1081 cccccggtag agttgcccct actccggttt tcgagaccgg tccgttcagc cgctccggca
     1141 tctctccgtt cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtc
     1201 cgtgcaattt tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa
     1261 agtacaagaa tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct
     1321 tttaacgtgt ctgacagaac aggaacaaag caaattggtc gctcttgccg aacgtttttt
     1381 acagcaaaaa cggcttgttc ctttacaggg ctttgagctg aattcattaa gaagctgccg
     1441 gatagcactt ctattttgcc tgcccgttct ggagttagga ctggaatggc tggatggttt
     1501 tcatgaagtc ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat
     1561 cggtctggtg cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat
     1621 cgttttgaac tggttggata tacaagattc ttttgatgct tctggtttta acctgattat
     1681 tcatgaagtc gctcataagc tggacacccg taacggcgat cgcgccagcg gagttccctt
     1741 tatttcgttg cgtgaggttg ctggctggga acacgatctt catgctgcaa tgaacaacat
     1801 tcaggaagaa atcgaattag ttggtgagaa tgcggcgagc attgatgctt atgctgccag
     1861 tgatcctgct gaatgttttg ccgtactttc tgaatatttc tttagcgccc cagaactttt
     1921 tgctcctcgt ttcccttcat tgtggcaacg tttctgtcaa ttttatcaac aagatccttt
     1981 gcagagactg catcacgcta atgatacaga ctcgttttcg gcgacgaatg ttcattaatt
     2041 aacaactttg cagattaatt aaccaattga aatggcttat gaaatttagt gttgacagac
     2101 aaggtaccgc taagtaatat gcgcc
//
LOCUS       DAZFRG010000429         2122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFRG010000429 DAZFRG010000000
VERSION     DAZFRG010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.431"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..450
                     /locus_tag="ACHKUC_004981"
     CDS             67..450
                     /locus_tag="ACHKUC_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFO6621040.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..957)
                     /locus_tag="ACHKUC_004982"
     CDS             complement(514..957)
                     /locus_tag="ACHKUC_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6621041.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="ACHKUC_004983"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="ACHKUC_004983"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO6621042.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          536 a          559 c          472 g          555 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaacccaa ccggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggctgtaca ggccgcgctc
     1561 aatatcctct atggtattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattatac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgcatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccgggctaga cccggatgta ccgtataact atttcccgca caatgatccg
     1921 caaaatacac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa ca
//
LOCUS       DAZFRG010000430         2106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFRG010000430 DAZFRG010000000
VERSION     DAZFRG010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.432"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1041
                     /locus_tag="ACHKUC_004984"
     CDS             <1..1041
                     /locus_tag="ACHKUC_004984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621043.1"
                     /translation="QEAFENAKVHVEKALNTVYQTNDDLYAKYAASFNKPAGNVDGST
                     TLWKPVLNKNNGAYFSSAKNAVGEFKYIYNGLNDTDIISTKYSIGGTGRSMTTRNDMD
                     ILTGEGDDIIAVGQDIGRSYSTGYTDKKYLTDMGDGNDILFVGLSNENVQINLRADGS
                     LRAIKKGDYFTSGEIINVSGDYDSDSGGVISSTTIKMGNGDDTILALGWSDGGNAVQN
                     SIIELGSGNDTMQVNGTIEASKIDGGAGFDTLIISNGTISSESFTGFEKIELMSSGHL
                     VLHSKDFSNHDIGGILKVTGETGSSVNIQDFNWQQGRLGRIEEDGVSYTTYISAEYPD
                     LSLWVQDGIDIR"
     gene            1100..>2106
                     /locus_tag="ACHKUC_004985"
     CDS             1100..>2106
                     /locus_tag="ACHKUC_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621044.1"
                     /translation="MGLFDYKDYHQAKITELVASSYNIAVYSNKGKVLGLGSFLAFRL
                     FSKHPDRIDYSPDVLNEGWRDITVEELGLSSEYVDSRGFYHFPSPVTGNPSSGFGPQL
                     KVVGLFDTQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLKSIRDFAVNNG
                     LSGEDIIVTGYSLGGGLTNIMARLSESLVDGFYVNSDYIAWASPLVFDGDGRVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSATDNIVLYTDLYGSFLWNVVPFS
                     VLALPLGSWNAHFAGSKTDAFARIWGSQFYNYMSPDSTVIVDEQSSWTSWYSWVHDKG
                     D"
BASE COUNT          630 a          342 c          501 g          633 t
ORIGIN      
        1 caggaagcat ttgagaatgc aaaagttcat gtagaaaagg cactaaatac tgtctatcag
       61 acaaatgatg atctttatgc taaatatgct gcgtctttca acaaaccagc agggaatgtg
      121 gatggaagca ctactctttg gaagcctgtc ctgaataaaa ataacggtgc ctatttttca
      181 tccgctaaaa atgcggtagg agaatttaaa tatatatata atggtttaaa tgacactgat
      241 attatttcta ccaaatactc aataggcggt actggtagat caatgacaac ccgtaatgat
      301 atggatattc ttactggtga aggtgatgat attattgctg taggtcagga tataggacgt
      361 tcgtatagta ctggatacac tgataagaag tatctaacag atatggggga tggtaatgat
      421 atcctgttcg tcggtttatc caatgaaaat gttcaaatca atctacgtgc agatggtagt
      481 cttcgcgcta ttaaaaaagg ggattatttc acctcaggag agataatcaa tgtatctggt
      541 gactatgatt ctgatagtgg aggtgtgata tcttcgacta ccattaagat ggggaatgga
      601 gatgatacga tattagcctt aggatggagt gatggtggta atgctgtgca gaactcaatt
      661 atagaactgg gatctggcaa tgacactatg caagttaacg gaaccattga ggcctctaaa
      721 atagatggtg gtgctggttt tgatacatta ataattagca atggcactat atccagtgag
      781 tctttcactg gatttgaaaa aatagaacta atgtcatcag gccacttagt cttgcatagt
      841 aaagatttta gtaatcatga tattgggggg attttaaaag ttactggaga gactggttct
      901 tcagtcaaca ttcaagattt taactggcaa caaggtagac taggacgtat agaggaggat
      961 ggagtaagct atacgacata tataagtgcc gaatatcccg acttgtcttt atgggttcag
     1021 gatggcattg acatccgata atatataacc ttaaagaaca gcctcagcat ggggctgttc
     1081 cttaaataag gaaatagtca tgggactctt tgattataaa gattatcatc aagcaaaaat
     1141 aacagaactg gtcgcttcgt cttataatat tgcagtatat tccaataaag gaaaggttct
     1201 gggattagga agttttcttg ctttccggct attttcaaaa cacccagaca gaattgatta
     1261 ttctcctgat gtccttaatg aaggctggcg tgatattaca gtggaggagc tcgggttatc
     1321 ttcggaatat gtcgattcaa gagggtttta tcattttccc agtccggtta caggaaaccc
     1381 ttcatcagga tttggtcctc agttgaaagt ggttgggttg tttgatacac aacagaatct
     1441 acagaaaatt gcaatatcat tctgtggaac aaatgatatc tgcgatattg ttgattatac
     1501 acagctcaat aatggaaaag cactatctga agcagaaaac ctgcttaaaa gtatcagaga
     1561 cttcgcagtc aataacggtt tgagtggtga agatatcatt gttactggct atagtctggg
     1621 cggcgggttg actaacatta tggccaggct cagtgaatct ctggttgatg gattttatgt
     1681 taattctgat tatattgcat gggcttcccc gctggtattt gacggagatg gaagagtatt
     1741 aaatatcggc tttgaaaacg atccggtatt tcgtgtgctg ggagatgagt ccgggttctg
     1801 ggaggctgtt aaagccatgg ggccaggatt gacaaccccg gatcgcgaat atgattctgc
     1861 tacagataat attgttttat acacagacct ttatggttca tttctctgga atgttgtgcc
     1921 cttttcagtg cttgctctgc cgctgggaag ctggaatgcg cattttgcgg gttcaaagac
     1981 tgacgccttc gcaagaattt ggggatctca gttttacaac tacatgtcac cggacagtac
     2041 agttattgtg gatgagcaaa gtagctggac atcctggtat tcatgggttc atgacaaggg
     2101 ggatga
//
LOCUS       DAZFRG010000431         2088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFRG010000431 DAZFRG010000000
VERSION     DAZFRG010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.433"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1243
                     /locus_tag="ACHKUC_004986"
     CDS             <1..1243
                     /locus_tag="ACHKUC_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621045.1"
                     /translation="GPAYEYYPDGKLMQENFYQQSELIGKDTSYYQSGKINSIHNRNS
                     RGQYDGINERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQ
                     WYKNGNLAKSQNYKHDILDGDSEEWYENGIPESLYPYKNGKTDGVAKSWNKYGKLTYS
                     IEYKNGVENGVYRNWSKNTGKLTKETQYVNGIRQGVEKEFNDRTGKLLTATQYVNNKR
                     NGTEETYDQNGIKYITCYQNDEELSSLYTPTQIKDNATKGNSSAQFTLGKYEFTCANI
                     DEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYITYLQQAAMLGNSNAQAEIG
                     YLYLIGKELPQNLPDAGVWFKKAAAQGNFVAHFYLGRMYQNGDGVEKNMEKARFHLSN
                     AAEGGIKPALKALNELEHQTK"
     gene            1296..>2088
                     /locus_tag="ACHKUC_004987"
     CDS             1296..>2088
                     /locus_tag="ACHKUC_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6621046.1"
                     /translation="MKVKSTLLLIILSVFYSHQVLSSAPTQPDVYSVVEQKLNSALPL
                     KENTTFRSQAEWFNLQYKLLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEGA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPDKAMQLLSLDDNALHYYLPTVLV
                     AYQSDPDKAISIYNNVYGDNVIIPYQQATMLLTVAEGYQTKGDIKNAIHYADNALNMF
                     GSRESHYGDEYLKLMNIYATNGNKEKAVVLSQRLQKAITESQSNYL"
BASE COUNT          768 a          373 c          408 g          539 t
ORIGIN      
        1 tggtccggca tatgaatatt atcctgatgg caaactcatg caggaaaatt tctatcagca
       61 aagtgagttg attggaaaag ataccagtta ctaccaatca ggaaaaataa actctataca
      121 caatagaaat tcccgtggtc agtatgatgg aattaacgaa cgttatagtg aggagggtaa
      181 gttactgtct aaatccgtct ataaagacgg aaaacaaatt tctgttcaaa cctggtatga
      241 aaatgggcag aaagaagagg agaaacattt tgatgagcaa ggccaattaa atggtttggt
      301 taagcaatgg tataaaaatg gaaatcttgc aaaatcacaa aactataaac atgacatact
      361 cgatggtgat agtgaagagt ggtacgaaaa tggcatccct gagagtctgt atccttataa
      421 gaatggaaaa accgacggcg ttgccaaatc atggaataag tatggtaaat taacctattc
      481 aatcgaatac aaaaatggcg tagagaatgg ggtatatcgt aactggagca aaaacacagg
      541 taaactaacc aaagaaaccc agtacgtcaa tggtatcagg cagggcgtgg aaaaagagtt
      601 taacgatcgc acgggtaaac tattaaccgc cacgcaatac gttaataata aacgaaatgg
      661 cacggaggaa acttatgatc aaaatggaat aaaatatatt acgtgctatc aaaatgatga
      721 ggaattatcg tctttataca ctccaacaca aataaaagat aatgcaacaa aaggcaactc
      781 cagcgctcaa tttacattag gaaaatacga attcacctgt gcaaatatag atgaagggat
      841 taaatggtta accaaatcgg cagagcaaaa aaacactgat gcgatatact ttttagctac
      901 agcctacaaa ggcaatggca tacctgcaaa caatgaaaaa tacataacat atctgcaaca
      961 agcagcaatg ctgggcaata gtaatgcaca ggcggaaatt ggctatttat atctcatcgg
     1021 aaaagaacta ccacagaatc tgcctgatgc tggtgtctgg tttaaaaaag cagctgctca
     1081 gggtaatttt gttgcacatt tctacctggg aagaatgtat caaaatggtg atggcgttga
     1141 gaaaaatatg gaaaaagcca ggtttcatct atctaatgca gcagaaggtg gaataaaacc
     1201 tgcgcttaaa gcattaaatg aacttgaaca tcaaacaaaa taaagcgttc agggaccatg
     1261 taaggttcat taaacagaaa aggatatgga ttgcgatgaa agtgaaatct actttgttat
     1321 taataatttt atctgtgttt tattctcatc aggttttatc cagcgccccc actcagccgg
     1381 atgtttattc tgtcgtcgag caaaaactta actctgcttt acctctgaaa gaaaatacta
     1441 ccttcagatc gcaggctgaa tggttcaatt tgcaatataa attattagta tctggctacc
     1501 ctgaacgtgc ttacaattta ctgagcaaac ttgaaacaga atcgaaaaac gctaaatatt
     1561 atctagattt aactgcctcc agagtcgctg aagaaggagc accagagact gccttaactt
     1621 ttttatcaaa aattgggtta aataaaccgg caactcttac atcctttgag agctatatca
     1681 atagctggat aaaaagcaaa caacccgata aggctatgca acttctcagt ctcgatgaca
     1741 atgccctgca ttattattta cctactgtgt tagtcgcata tcagagcgat cctgacaagg
     1801 caataagtat ttataataat gtatatggtg acaatgttat tataccctat cagcaagcca
     1861 ccatgttact tacagttgcg gagggttatc aaacaaaagg cgatataaaa aacgctattc
     1921 attatgccga taatgcgctg aatatgtttg gcagtcgtga atcccattat ggtgatgagt
     1981 atttgaaact catgaatatc tacgctacca atggcaacaa agaaaaagcg gtagttttat
     2041 ctcaacgtct acaaaaagcc atcaccgaat ctcaatcgaa ttacttaa
//
LOCUS       DAZFRG010000432         2087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFRG010000432 DAZFRG010000000
VERSION     DAZFRG010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.434"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..878)
                     /locus_tag="ACHKUC_004988"
     CDS             complement(<1..878)
                     /locus_tag="ACHKUC_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO6621047.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCMDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHY"
     gene            complement(882..2039)
                     /locus_tag="ACHKUC_004989"
     CDS             complement(882..2039)
                     /locus_tag="ACHKUC_004989"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFO6621048.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDVLGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
BASE COUNT          492 a          568 c          547 g          480 t
ORIGIN      
        1 ttataatgac gaataatttg ttgaatctcg ctgatctgtt caggcgtgaa attaaccgcg
       61 tagcggctat caccaacatc atgccccata agctgcaacg cttttttcac cgcagccggg
      121 gagaatgcta ctttataaag atcggtacgc aggcccgtta cactctcctg tgcttcataa
      181 gacccttcaa tatcaccggc atggaagcga gaccagatgg cattgatttc tcgcggcgcg
      241 atgttggcga gcccagagat acaggctgaa catccctcga caaacccttt atggatcagc
      301 gaatccgggc cgttgagcac attaaagccg tcaatgtcgc gcaccgcatc gagaaaaccc
      361 ttcaggcttt cgtcgcttcc ggcgctgtct ttaatcccaa tgatattggg gtgcgacgcc
      421 agagcacgcg cggtttctgg ctggatcgta ttgccagtgc gggccgggat gttatagaga
      481 aacatcggaa tgctcagtgc gtcggcaatt gcgctgtaat gcgcaatcaa ctcttcctgt
      541 ttcagcggta cgaagtaagg ggcaatgacc gacacggcat ctacgccaag tttttcaatc
      601 tgctgaccca gacgaatcgt ttcgcgcgta gagatctcgc caatgtgtgc cacgaccggc
      661 gcacgaccag ccacttcatc catgcaggtt tgcgtaacgc tgagcttctc ctgttcattc
      721 aacacaaaaa actcgccgtt ggtgccgccg caaaaaatcg cgttacccgc ttccagttga
      781 cgattcacct gcacttttaa cgccgggata ttcagactgc cggtcgcggt aaacggggtg
      841 acgatagcgg ttaatacacc ttcaatggtt ttcggcatga tttattcctt aaattgatgt
      901 tgcggaaaca gtgtttggta aatggcctgt acgtcctgta gatcaagctg gcacggcaca
      961 ttgttcataa ggcgcttcac gttcagtgcc gcctcgctaa gagaggcaat atcctctggc
     1021 ggtacgccga gatccgcgag attgttgggt agcttgagtt gattgactaa tgcctgtaac
     1081 caggtcacca gggcatgaga tttttcttcc gcgctcaggg cggtttccgc atcgggaatg
     1141 caatcccaga cccgggcaaa tttctcgacc gcccaggggc gaacaaacgc catgcacggc
     1201 gccagcaaga tggcattcgc gacgccatgc ggcagatgat atttgccacc taacgggtag
     1261 gagagcgcat gaacgagatg cgtgcccgca tgggttatcg ctacgccgcc atagtaagac
     1321 gcccacagca tttccagttt tgcgcgcaga tcctgaggat cgttcacggc gggttgaatg
     1381 tgccggaaaa gtttacttaa cccagtcagc gccgcgttat cgctgaccgg atttgccacg
     1441 gtcgcggtaa aacactccag taagtggcaa agagcatcaa tgcccgtgga cgccgcaata
     1501 tgcgcgggca tgctggtggt cagttccggg aaaagcgcca catagtctgg taacagcacc
     1561 tgggaaataa tacccacctt cgtgctttgc tcagggattg ccagaatcgc attcggcgtg
     1621 gcttctgagc cggttccggc ggttgtagga atcaaccatg attgcacccg ctgagtcggt
     1681 ttttcacccg caagcagcgc atccagcccc ggagattgtg gatggcaaag caccgatagc
     1741 agctttgcca catccaacac gctgccaccg ccgatcccga ccaccacatc aaaagaggca
     1801 tcgccaagga catccattag ctggcgcaca tcatgatgcg tgggctctgc gggcacatta
     1861 tcgataacgt taacctgcgg gcagtgcttt tccagtaagg cgcgaatctg ctgcacaccg
     1921 tcgagctgcg caatattacg gtcagtgacc agcagcattt ttctgatgtc gggtaagagc
     1981 gacgtcaggg aagggatagc gcctgcgccg ctcagtatcg tgctattgat aattaccaca
     2041 aataacctct cttctggtga ttgaattcaa tcattcaaaa tgccgta
//
LOCUS       DAZFRG010000433         2053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFRG010000433 DAZFRG010000000
VERSION     DAZFRG010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.435"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..579
                     /locus_tag="ACHKUC_004990"
     CDS             22..579
                     /locus_tag="ACHKUC_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6621049.1"
                     /translation="MVLLNNKRKSKKGFSLLELLLVLGIIAALVVAAFIVYPKVQASQ
                     RAQAESNNIATIQAGVKALYTSASSFTGLTNTVAVQAKIFPDNMLSGSGTAAKPINAF
                     KGNVTLAATATGPSSAAGSSFTITYDNVPAAECVKIATAAAGNFYITTVGTKVVKAAG
                     GTLDVAATAAACTNATSNTLVFTSI"
     gene            624..2053
                     /gene="pilV"
                     /locus_tag="ACHKUC_004991"
                     /pseudo
     CDS             624..2053
                     /gene="pilV"
                     /locus_tag="ACHKUC_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592083.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
BASE COUNT          622 a          397 c          505 g          529 t
ORIGIN      
        1 aaaacaaaaa aggagataat aatggtttta ttaaataaca aaagaaaaag caagaaaggt
       61 ttctcactac tcgaactatt acttgttttg ggcatcattg ctgcattagt ggtagcggct
      121 tttatcgttt atcccaaggt tcaggcttca caaagagcgc aggcagaatc caataacatt
      181 gccaccatac aggcaggtgt taaagcgtta tacacatcgg cttcaagctt tactggcctg
      241 acaaatactg tggctgttca ggcaaaaatc ttccctgaca atatgttaag tgggtcagga
      301 acggctgcta aacccatcaa tgcctttaaa ggtaatgtga cgctggctgc gacagctaca
      361 gggccatcat cagcggctgg atcttctttc actatcacat acgataatgt gccagcagcg
      421 gaatgtgtga agattgcgac agcggcagcc ggtaattttt atattactac ggtaggaacc
      481 aaggttgtta aagccgctgg aggaacactc gatgtggcag ctacggcagc agcatgtacg
      541 aatgccacaa gcaatacatt agttttcacg tctatttaat gaagagggcg caggcccttt
      601 tctttcttat tgctaaaaca gggatgataa agaaaaaagg atttacctta ctggaagtga
      661 ctattgtttt aggtattgga acgcttatcg cttttatgaa gtttcaggat atgagaaacg
      721 atcaggaagc agtattggct gataatgtcg gaacacagat taaacaactc ggtgaagcag
      781 ttaaccgcta tatcagtatt cgctatgaca agatttccac actgtcatca tccaacaatc
      841 aaagcagtga tccggggcca agaacctgtt cggctgctgg ttgtgaaatc acttaccaga
      901 cgttaattaa tgaaggtctg ttgcctgtgg gctatacagg aacgaatgcg caaaaagcat
      961 cctacaaaat tgtgttaaag cgttcaggaa cagcgccgga ttacgtcatt aatggcctta
     1021 taacaacaac cagtccgtgg acagaaggaa agaacattcg ctatgacctg ttaggaaaag
     1081 cggttcagtc ggcaggtgtt gatggtggga tgagcagaaa caccaagata gcgtccggct
     1141 atggtggtca gtggagtgag aattctaata gctacagcaa tatcacgggt ggtggattgc
     1201 tggcttaccg tgtagggtat aactcttcaa tgtattctgt ttacctgcgt cgtgatggaa
     1261 cgttgccgat gacaggtgat ctgaatcttg gtgggaagag catcaaaaac atcaaagata
     1321 ttacggcatc agggacaaca acaaccggag cacttaagac aacgggtaat gcgtctgttg
     1381 catccgatct tggtgtgggt ggaacatcta cccttaatgg tcctgtaaat atcaacaaca
     1441 acctgaaggt aaaatccgat acctatctga acacgctatc tacaaccgga ctggctaagt
     1501 tcggaggacg tatagcaacc aacggactta atccaaacga tttgcctgct gggtgggctg
     1561 gcggtgttcg cacctatgat ttgtatgcgt cagggactgt tggtgtagga accggtaaaa
     1621 cagttaaagc ttacatgaac aatgcgggta atatttacgc gtcaggtaat ctaactgccg
     1681 gaacgataaa atctaatggc acaattgaat caactggcag aatcaaggct ggtgaatatc
     1741 ttcatttgaa tggtcaggca acgttaaatg cgaaatgtac gcctaatggt ctggtggggc
     1801 gagacagtac aggtagagtc ttatcgtgtg ttaacggtaa atggcagaca gcaagcggtg
     1861 atggcctgaa aggtattttt ataacaataa ccgatcaaac atctggatat aaatgtgtaa
     1921 taccgaatag tgacactggg acatgtgcct gcccggatca atgtatagtc ctcagtttgg
     1981 ttatatatct ggaacactta ttgcggaata tgatgaacgt cgttgtagtg gtagtaagaa
     2041 cgagcactgt tat
//
LOCUS       DAZFRG010000434         2053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFRG010000434 DAZFRG010000000
VERSION     DAZFRG010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.436"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2053)
                     /locus_tag="ACHKUC_004992"
     CDS             complement(<1..>2053)
                     /locus_tag="ACHKUC_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6621050.1"
                     /translation="NRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSS
                     DSNKWQHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHG
                     FAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGS
                     IQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGNNLQSTPKFVQASLMHGLKGNWTPYG
                     GMQIAEDYQAFNLGIGKDLGLFGAFSFDITQANTTLADDTRHSGQSVKSVYSKSFYQT
                     GTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDTSEQTLFIDYFNLFYSKRGQ
                     EQISISQQLGNYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIW
                     QNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPD
                     NNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAYANTNVGYSRSGDSSQIYYGMSGGII
                     AHADGITFGQPLGDTMVLVKATGADNVKIENQTGIHTDWRGYAILPFATEYRENRVAL
                     NANSLADNVELDETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHG
                     ENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPVVSPGTLLNQQTAI
                     CR"
BASE COUNT          551 a          472 c          442 g          588 t
ORIGIN      
        1 gcgacagatt gctgtctgct ggttcagcaa ggttccagga gagactacag gaagcttgta
       61 atcgacaata cagtttgagt ttttatcatt gccccatgaa acctgtaatt tccctgactg
      121 tggaagtcca gtcagataaa cctgaccatt ttccgcgaca atgctgccat ttttattctc
      181 tccgtgagtg acaattgcac cgaatggaac gcttttatta ccgtacttca acgtcattaa
      241 tactttcccg ccgatttgtg cattaaatgt tgctctggca atagcaccgt gagttgggat
      301 gacagtgact acggtttcat ccagttcaac attatctgca agggaattcg cgttaagagc
      361 gacacgattt tctctatatt ctgtcgcaaa tggtaatatg gcatagccac gccagtcggt
      421 atgaattccg gtctggttct ctattttgac attatcagcg ccagtagcct taaccagaac
      481 cattgtgtcg cccagcggct gtccaaaggt gatgccatca gcatgagcaa taatcccacc
      541 actcattccg taataaatct ggctgctgtc accactccga ctgtaaccga cattcgtatt
      601 agcgtaagct ccacgataat taagagaact gtaaccactg gtgccagacg atgtattacc
      661 cccttgggtg ttaccgacct gaacgctata attcaggtta ttatccggca gcagagtgcc
      721 ataaacccct gataggttgg tcatgccgcc tttcaaatcg tttgacatac tgtaactggc
      781 gtttgaatta cgaaatgccg actgactgtc tgtacgcatc caatgactaa agggaacatt
      841 aagcgtaaaa gcgagtaaat gatcccgatc gttttgccat atattattgg aatagctgta
      901 attcagtgaa gtcgtaatat caccaaacgg cacatttaat ccaaatgata tttgctggtc
      961 gctgcgtgac gtgttccagt aactttggcg actggcactg aaaaatgtcg taccgtaatt
     1021 tccaagctgc tgagagatgc ttatttgttc ctgaccacgc ttactgtaga acagattaaa
     1081 ataatcaata aatagtgtct gctcactggt gtctccggta ggaggcttga cggtgtaacc
     1141 actcattcga ctgtatgcac tgtcggataa gttataaaaa ccttgcgtag aatagcgata
     1201 tcctgcgacc tggatattgg ttcccgtctg gtagaaggat ttgctgtaga cggatttaac
     1261 cgattgtccg ctgtgacggg tgtcatctgc aagtgtcgta ttggcctgcg taatatcgaa
     1321 agaaaaggca ccaaaaagtc ctaaatcttt accaatacca aggttgaagg cctgataatc
     1381 ttctgcaatt tgcattccgc cataaggtgt ccagtttcct ttcagaccat gcatcaagct
     1441 tgcttgtacg aacttggggg tgctttgcag gttatttcca ctacgatatt cccccatggc
     1501 aagtgcataa cgcgtatatc cagcacgttg gagaaccgga acagacgaat aaggaacata
     1561 taatgtctga atactgccgt ctgcctcttt tatggttact tgcagatcac caccattggc
     1621 cgcagagttg atatcatcaa tagtaaaagg gccgggtggg acagtagtct gataaacatc
     1681 gtatccattc tgttttacac tcacttgtgc ggtgccacga gcaataccat gaatcaccgg
     1741 agcgaaacca tgttggctat cgggcaacat cgcttctgtt gaatttattt taaggccacg
     1801 aaagttcaca ctgtcaaaaa tatcgccatc ggtataacta tcacccactg ttaaccgtga
     1861 gcgtaacgga ataatgtcac gttcagccga cgtattgata tgctgccatt tattgctgtc
     1921 agatgaattg ctactcccac tgttataact ccacgttgag ttatcgcgca gtcgccaact
     1981 accaatgttg atgccactct gtaaattcaa atatgcatag ttggattttc ctgcattatg
     2041 gttactacgg tta
//
LOCUS       DAZFRG010000435         2046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFRG010000435 DAZFRG010000000
VERSION     DAZFRG010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.437"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(427..615)
                     /locus_tag="ACHKUC_004993"
                     /pseudo
     CDS             complement(427..615)
                     /locus_tag="ACHKUC_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            676..1809
                     /locus_tag="ACHKUC_004994"
     CDS             676..1809
                     /locus_tag="ACHKUC_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO6621051.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLTAITLVAELGDFSRFSSPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRSLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
BASE COUNT          613 a          447 c          443 g          543 t
ORIGIN      
        1 ttcgagctaa gagctaaatg taacaacact aaaatatatt tattcttaca aaacccatag
       61 aagcggcata tttctatggg ttttctttct accagagatt gtgtgactga caaatttaat
      121 gagatgcaaa attttgatgc tcctaatctc aaatatcatt atagccttct attaggatga
      181 atgaaaatac tataattatt aaaagttaga tataaaaaat aagataacta aaaatacaga
      241 aaccacagaa cccttgtggc atctggaaac cagactttta gcgatacgga atagtttatt
      301 aaagataaga aagtagatat tgttatctgt ttatgataac attgaattat tataacataa
      361 atatcttcac acatacaact ccccgccgac ttattggtaa gcagctcgtc agaactgtat
      421 tgatatttac tgagagctca gatcaacttt ccagggcaac agatcgcgta cccgatttgc
      481 cggccagtcc tggatatgtt caatgacgta acgcagccat ttttctggct ccacattgtt
      541 cagacggcag gtgccgatca gcccgtacag cagtgctcca cgctcgccgc cgtgatcgct
      601 gccgaagaac ataaagatgg tatgaccttt atatactcac tatagcattg gtgggatatt
      661 ccagaggagg tcattatgca ttcggaaaat attgcagctt acatcggact ggatgtacat
      721 aaagaaacgt tggcggttgc tattgctgca cctgaacggc ttggcgaagt tcgttattat
      781 ggtacgatta acaacgaagc ccaggctgta cgtcgattat ttcagaaatt gcagggactt
      841 tacggaaaca ttctttcctg ctatgaggct ggcccctgtg gttttgggct atatcatcaa
      901 ctcacagcga tgaatataaa atgccaggtt attgcccctt cccggatacc aaaatcaccg
      961 acagacagaa tcaaaaatga tcatcgggac gcaatttctc ttgccaggtt actgcgggct
     1021 ggtgaactta cccctgtctg gatccctgac ctgactcatg aagctatgcg ggatctaatc
     1081 agagcccgtg cagcagctaa acgagacagc agagttgccc gacagcgaat tctgagcatg
     1141 ctattgcgaa cagacaaacg ttatgcaggg aagcactgga ccggcaaaca tcgaacctgg
     1201 ttagccaatc aatcatttag tcagccatct caacagattg cttttcaaca ctattgtcag
     1261 agtctggagc agattgaaga tcgcatactt caactcgacc aggaaatatc ccgcctgtta
     1321 cctgagtggt ctctgtgcaa tctggtctgc cagttacagg cccttaaagg tgttggtcag
     1381 ctaaccgcaa taaccctggt tgctgaactg ggtgattttt cacgattttc cagtcccaaa
     1441 caactcatgg cttttctggg actggttcca ggggaatatt ccagtggaaa cagcattcgt
     1501 cccaggggaa taacaaaagt tggaaacagc gaactgagac gtctgcttta cgaagccgcc
     1561 tggtcttatc gtacacctgc aaaagttggt gcatggctta tatattaccg accggattct
     1621 gtaacacaat attccaaaga tattgcatgg aaagctcaac aacgattgtg ttctcgttac
     1681 cgaagtctga cagcaaaagg gaaaaaatca caagtagcca ttacggcggt ggctcgtgag
     1741 ttgactggat ttatgtggga tattgcactt gctgcccaat catcattcag tcagcagaag
     1801 caaaattaaa ccctgaagca gtcagacacg gaatgagtga tcctcgtaaa agaattcagg
     1861 aggtcagaat gcgccaatac ctgcatatag aaagaggcca gctcactgcg taaatcgtca
     1921 tggggctaaa atccccgaat aagagtttgt aaaatccaag atacagtctg actgtttcag
     1981 gttctttaat ccgcgatata acgaagctcc cggagctgcg taaggtcttt ttgggctcaa
     2041 tatttg
//
LOCUS       DAZFRG010000436         2001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFRG010000436 DAZFRG010000000
VERSION     DAZFRG010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.438"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1796
                     /gene="sat"
                     /locus_tag="ACHKUC_004995"
     CDS             <1..1796
                     /gene="sat"
                     /locus_tag="ACHKUC_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HFO6621052.1"
                     /translation="TKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVI
                     AQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASV
                     TGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNN
                     AIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQLV
                     VNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTPVI
                     EQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDING
                     ESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGSDA
                     FSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYAGA
                     EVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVG
                     KSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIR
                     DNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT          566 a          455 c          477 g          503 t
ORIGIN      
        1 aaactaaatt tacaggtggc atcactgcac ataacagcca aatagccata ggtgatcaag
       61 ctgtagttac acttaatggt gcaacctttc tgaataatac tcctataagt atagataaag
      121 gagcaaaagt tatagcacaa aattccatgt tcacaacaaa aggtattgat atctccggtg
      181 agctgactat gatgggaatc cctgaacaga atagtaaaac tgtaacgccg ggtctccact
      241 acgctgctga tggattcagg ctgagtggtg gaaatgcaaa tttcattgcc agaaatatgg
      301 catctgtcac cggaaatatt tatgctgatg atgcagcaac cattactctg ggacagcctg
      361 aaactgaaac accgactata tcgtctgctt atcaggcatg ggcagagact cttttgtatg
      421 gctttgatac cgcctatcga ggcgcaataa cagcccccaa agctacagtt agcatgaata
      481 atgcgatctg gcatctaaat agccagtcat caattaatcg tctagaaaca aaagacagta
      541 tggtgcgttt tactggtgat aatgggaagt ttacaaccct tacagtggac aaccttacta
      601 tagatgacag tgcatttgtg ctgcgtgcaa atctggccca agcagatcag cttgttgtca
      661 ataaatcgtt gtctggtaaa aacaaccttc tgttagtcga cttcattgag aaaaatggaa
      721 acagcaacgg actgaatatc gatctggtca gcgcaccaaa aggaactgca gtagatgtct
      781 ttaaagctac gactcggagt attggcttca gtgatgtaac accggttatc gagcaaaaga
      841 acgatacaga caaagcaaca tggactctga tcggctataa atctgtggcc aacgccgatg
      901 cggctaaaaa ggcaacatta ctgatgtcag gcggctataa agccttcctt gctgaggtca
      961 acaaccttaa caaacgtatg ggtgatctgc gtgacattaa cggtgagtcc ggtgcatggg
     1021 cccgaatcat gagcggaacc gggtctgccg gcggtggatt cagtgacaac tacacccacg
     1081 ttcaggtcgg tgcggataac aaacatgaac tcgatggcct tgacctcttc accggggtga
     1141 ccatgaccta taccgacagc catgcaggca gtgatgcctt cagtggtgaa acgaagtctg
     1201 tgggtgccgg tctctatgcc tctgccatgt ttgagtccgg agcatatatc gacctcatcg
     1261 gtaagtacgt tcaccatgac aacgagtata ccgcaacttt cgccggcctt ggcaccagag
     1321 actacagctc ccactcctgg tatgccggtg cggaagtcgg ttaccgttac catgtaactg
     1381 actctgcatg gattgagccg caggcggaac ttgtttacgg tgctgtatcc gggaaacagt
     1441 tctcctggaa ggaccaggga atgaacctca ccatgaagga taaggacttt aatccgctga
     1501 ttgggcgtac cggtgttgat gtgggtaaat ccttctcggg taaggactgg aaagtcacag
     1561 cccgcgccgg ccttggctac cagtttgacc tgtttgccaa cggtgaaacc gtactgcgtg
     1621 atgcgtccgg tgagaaacgt atcaaaggtg aaaaagacgg tcgtatgctc atgaatgttg
     1681 gtctcaacgc tgaaattcgc gataatcttc gcttcggtct tgagtttgag aaatcggcat
     1741 ttggtaaata caacgtggat aacgcgatca acgccaactt ccgttactct ttctgataac
     1801 agcccgggcc gcgtttgcgg cccttcttct accggagaga atatgtatta ccctgtgaca
     1861 gactatatcg ctcttgctct cattattagc tttctttttc tgacattatt tatctgcctg
     1921 ttatgcctta aacatgagcg aataaaaaaa gaaactatca ggcaaaaaaa tgcacatatt
     1981 ctggatcatg gctggaatgc a
//
LOCUS       DAZFRG010000437         1998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFRG010000437 DAZFRG010000000
VERSION     DAZFRG010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.439"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..257
                     /locus_tag="ACHKUC_004996"
     CDS             <1..257
                     /locus_tag="ACHKUC_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621053.1"
                     /translation="IEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILESQFRL
                     SVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(579..1181)
                     /locus_tag="ACHKUC_004997"
     CDS             complement(579..1181)
                     /locus_tag="ACHKUC_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621054.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVFWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
     gene            complement(1276..1482)
                     /locus_tag="ACHKUC_004998"
     CDS             complement(1276..1482)
                     /locus_tag="ACHKUC_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6621055.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1801..1998
                     /locus_tag="ACHKUC_004999"
                     /pseudo
     CDS             1801..1998
                     /locus_tag="ACHKUC_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          476 a          497 c          560 g          465 t
ORIGIN      
        1 tcattgagga gtgccgcgga gaatgcccgc cccgtgtcag gaaaaattat attctccagt
       61 gtggcccggg ccggttcaga aacaggaaaa aactgaacgc attgctgaac attcttgaat
      121 ctcagttcag gctttctgtc gtgccggaag gaaaaaccat gtatgttctg cttccccaga
      181 tagccagttt aaagttgtct gatgtctctg gtatttttac ctcgggttat cactacaata
      241 agctgagagc gaagtaaccg gtgagcagtt tttctccatt atgcttcaga cttagatgat
      301 tgattattat ggggatttca tcaccgatat tcagatgtat gaatatcggt gatgaactaa
      361 agctcaggag ctgcgtggtg gtgatgaatc aacaggcata taactgatac ccgccggtca
      421 gaagttccag cctgcgactt tcctgtctgc gtgcccgcag tccggcgttc aggtgcagcg
      481 tgtgacagaa cgcacggaga gcgaaacggc aggcagacag gccgttcagt cagccctggc
      541 accctgcccg gataccagct tccctgaaac aactggcatc aggtgcgctt atcgtcaacc
      601 gttgtgcggt atgacaggag cacgtactga tccatcagat ccgtcagtgc tgcgctctgc
      661 ttctgacggt tgatgatcca ttcctccagc tctttttctg atttccgttt cagattctcc
      721 gtccagtcgt caaggcagtt tccggtcatt aaatcaagcc agcaaagcca gaccagtttg
      781 aggcgcaact ccgtgccacc aaactgacgg aagaggctga tcagtttatt tgccagagaa
      841 cgctgcctca gacgttcaca ttcagacagg agaatgcctt ccagcatcgg atgatgaacc
      901 agcagatgtt cactggtgca taaattcact atcagatcat catccagggg cggtgtggcc
      961 cgtttgtcag ggccataaat cagccggtgc aggctctggg gtacgatggc aatctccggt
     1021 ttttcaccat tgaatgacac ccaaccgggc gtcagattat ggaagtagta gatattcgga
     1081 tgacacacgc cccagaatac ggcgtgccgg gtgaagccgg tcagccattt ctggtagctt
     1141 tcgtggagcc attcgtagcg ggtctgatac tgttgtttca ttttcatatt cctcacgggg
     1201 atataacagg cagcagaatg ccgccggcag gccggagatt acgtctgctg cgggtgaacg
     1261 gataacggtc agaaattacg gacgggactg tgcaatacgc gcctgcagcc aggcttccac
     1321 ttcacttttc agccagcggg agctgcgccc cattttgatg ggggcaggaa agcccccatc
     1381 tttgatgagt ttgtaaaacc acttatcggt taagccggta agttgagtga taaacgccat
     1441 gtcgaccatc tggtcatcca tcagcgaaac aggggtagcc atgagaatgt tcctccggat
     1501 gttgttaaac cagccggtgt ggtcatgaaa acagcgcgcc accttcactg gcataacaac
     1561 cggggagaaa cgtaaaaaat aatcgggaat gtggctgtcc ggagataaac ggggggattg
     1621 cgatggcaga gccgtagcgg aatcccgggg ggttgttgcg aaaagatgct tccgcacgca
     1681 cgcgcgataa aggagggagg actttcgtga gacaagggga aaactagcgt tctgataatt
     1741 gcagagttgt tttcggaggt ggttacagtg agtcagcccg ttcctggcga ggaggagact
     1801 gataccgccg aacagctgga gttgatgcgt agcgccttcc gggttatccg gacggtacgg
     1861 gaaaaacatg cctgtactca gtgcgatgcc atcgtgcagg cacctgcacc ttcgcggccc
     1921 atcgagcggg gtatcgccgg accggggctg ctggcccgcg tgctgacctc gaagtatgca
     1981 gagcacaccc cgctgtat
//
LOCUS       DAZFRG010000438         1990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFRG010000438 DAZFRG010000000
VERSION     DAZFRG010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.440"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            213..1910
                     /gene="treA"
                     /locus_tag="ACHKUC_005000"
     CDS             213..1910
                     /gene="treA"
                     /locus_tag="ACHKUC_005000"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6621056.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQHQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSIENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT          560 a          510 c          477 g          443 t
ORIGIN      
        1 gctaatcgca aacccaacgt cgctggctga ctaataaaca gatgttattt tatcactcta
       61 tttcgccgtt atttatccct ttaattccct tttctaaaat gtctgtcagt tcgcagaatg
      121 tgatttcgat catgcagcta gtgcgatcct gaactaaggt tttctgatac ttgaataccg
      181 ttttattcgg tttcgccaaa ggagaatgac tgatgaaatc ccccgcacct tctcgcccgc
      241 aaaaaatggc gttaattcca gcctgcatct ttttgtgttt cgctgcgcta tcggtgcagg
      301 cagaagaaac atcggtaaca ccacaaccgc ctgatatttt attagggccg ctgtttaatg
      361 atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt tgccgatgcc gtgccgaaca
      421 gcgatccgct gatgatcctt gctgattatc ggatgcagca acaccagagc ggatttgatc
      481 tacgccattt cgttaacgtc aatttcaccc tgccgaaaga gggcgagaaa tatgttccgc
      541 cagaggggca atcactgcgc gaacatattg acggactttg gccggtatta acgcgttcta
      601 tcgaaaacac cgaaaaatgg gattctctat taccgctgcc aaaaccttat gtcgtgccag
      661 gcggacgttt tcgcgaggta tattactggg acagttactt caccatgtta ggacttgccg
      721 aaagcggtca ctgggataaa gtcgcggata tggtggccaa ttttgctcat gaaatagaca
      781 cttacggtca tattcccaac ggcaaccgca gttactattt aagccgctcg caaccgccct
      841 tctttgccct gatggtagag ttactggcgc agcatgaagg cgacgccgcg ttgaaacaat
      901 acctgccgca aatgcaaaaa gaatatgctt actggatgga cggtgttgaa aacctgcaag
      961 ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg tacccttctc aaccgctact
     1021 gggacgatcg cgatacgcca cgaccagagt catgggtgga agatattgcc actgccaaaa
     1081 gcaatccgaa tcgacctgcc actgaaattt accgcgacct gcgctctgcc gctgcgtctg
     1141 gctgggattt cagctcgcgc tggatggaca atccgcagca gttaaacacc ttacgcacca
     1201 ccagcatcgt accggtcgat ctgaacagct tgatgttcaa aatggaaaaa atcctctccc
     1261 gcgccagcaa agctgccgga gataacgcga tggcaaacca gtacgaaacg ctggcaaatg
     1321 cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca acaaggctgg tatgccgatt
     1381 acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc ggccgccctg ttcccgctgt
     1441 acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc gacggcgacg aaaacacatc
     1501 tgctgcaacc aggcggcctg aacaccacgt cggtgaaaag tgggcaacaa tgggatgcgc
     1561 caaacggctg ggcaccgttg cagtgggtcg cgacagaagg attacaaaac tacgggcaaa
     1621 aagaggtagc gatggacatt agctggcact tcctgaccaa tgttcagcac acctatgacc
     1681 gggagaaaaa gctggtggaa aaatatgatg tcagcaccac cggaacaggg ggcggcggtg
     1741 gcgaatatcc attacaggat ggctttggct ggaccaatgg cgtgacgctg aaaatgctgg
     1801 atttgatctg cccgaaagag caaccgtgtg acaatgttcc ggcgacgcgt ccactcagtg
     1861 aatcaacaac acagccgcta aaacaaaaag aggcggaacc tacgccttaa ctttactgta
     1921 tatcgagcaa tgccattacg tgcataaaat tggcattgct ctttcttcat cacgaaatac
     1981 caaataccac
//
LOCUS       DAZFRG010000439         1984 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFRG010000439 DAZFRG010000000
VERSION     DAZFRG010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.441"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..464
                     /locus_tag="ACHKUC_005001"
     CDS             102..464
                     /locus_tag="ACHKUC_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621057.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(567..1025)
                     /locus_tag="ACHKUC_005002"
     CDS             complement(567..1025)
                     /locus_tag="ACHKUC_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6621058.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1022..1939)
                     /locus_tag="ACHKUC_005003"
     CDS             complement(1022..1939)
                     /locus_tag="ACHKUC_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6621059.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
BASE COUNT          490 a          538 c          481 g          475 t
ORIGIN      
        1 gtttctgtgc taaacgtgaa gcggttcctg atgtgtgcta gaatgcgttt ctttgataaa
       61 tctcaatttt taagaggttt acggcaggca ggaaaattta aatgaatgaa tttgagaaga
      121 tttttaatga aatgaatctg gaccgggcat tgctaccgat cctttttcgt tcgaaccggt
      181 cgacagtgtg gaagtattta agcggcgata gtacggctcc tgcctctgcc atgtcgctga
      241 ttatgctgtt gcagttgatc cagaaacgaa accccgatct gttagccgaa tggttaacgc
      301 tttctgattt caccattccg ccagaagttt acctggacca acctgattac tggaaagggt
      361 gggtgtatac ccaacacaag gtcaataaaa acgtcctgga atatctaaaa aaacactatc
      421 cggatgaaga ccaaaaaagt atgggtaaag gccgggaaga gtaatttttc ctctactcgg
      481 ctatatgccg gatgcggcgc aagcgcctta tctggcctac gattcgggca tattttgtag
      541 gcatgataag acgcgccagc gtcgcatcag gcgatgaccg cacggataat cagcgttatt
      601 ccttctatcg caatcacctc aacatgcgta ccagcgccga gatcctcgct ggcgctgaca
      661 ggccatgaac tgtcaccgac gcgcatatga ccacgcccgt tgaccagcgg agattccaga
      721 acaaaacgtc ggccaatcaa ctgctgcccg cgctggttta aatgactgtc gctgtgcttt
      781 tgttcgcgca cccgccgcga caaccatttc caccacagcc aggcggcgag cagcgtcagg
      841 acggcaaaca ttaccccttg ccactcccaa cccagcggca gcaaccagac caccagaccg
      901 gtaatcactg ctgccacgcc gctccacaac agataaccat tgccgcccaa catctcggct
      961 gccagcagca aaccgccgag acttagccag aaaatatgtg gatgaacgac cattaactcc
     1021 atcatggctg agtccgtttg ttggcgctgt ctttcaccag ctcagcaatc ccggcaatcg
     1081 accccatcag gctgctggcc tctaatggca tcatcaccac tttgctgtta ctggacgaac
     1141 cgatctgctg taacgcttcg gtgtatttct gtgcgacgaa gtagttcacc gcctgaatat
     1201 caccggaggc gatggcttca gacaccattt tggtggcgcg ggcttctgct tcagcggaac
     1261 gttcacgcgc ttcagcctgt aaaaacgccg actgacgttc gccttccgct ttcaggattt
     1321 gtgactgttt ttcaccttcg gctttgagga tttccgcctg acggatccct tccgcttcaa
     1381 ggatgtaagc gcgtttggta cgttccgctt tcatctgcgc gttcattgaa gagataagct
     1441 ctgcgggcgg gcgcacatca cgaatttcga tacgggtgac tttaatcccc cacgggttgg
     1501 tggcctcatc gacaatatgc agcagacgcg agttaatgct gtcgcgctga gagagcattt
     1561 catcaagttc cattgaaccc agcacggtac ggatgttagt catggtcagg ttgatgatcg
     1621 ccagctccag attgctgact tcataagccg cgcgcggcgc gtcaatcacc tgaataaagc
     1681 acacagcgtc gatggtaacg ttggcgttat ctttcgagat aacttcctgg gaagggatgt
     1741 cgagcacttg ctccatcata ttgatcttgc gaccaatgcg atccataaac ggcaccacca
     1801 gactgagccc cggctgtaac gttttggtat agcgaccaaa acgttctacc gtccattgat
     1861 agccctgcgg cacgattttg acgcccgcgc cgacaatgac cagcgcgaca aaaatgagaa
     1921 tagggataaa gataagcatc ggaaaaacct cctgttgtac cgtccataat cagcaaaatt
     1981 gctg
//
LOCUS       DAZFRG010000440         1983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFRG010000440 DAZFRG010000000
VERSION     DAZFRG010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.442"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(110..1099)
                     /locus_tag="ACHKUC_005004"
     CDS             complement(110..1099)
                     /locus_tag="ACHKUC_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO6621060.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYNPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
     gene            complement(1508..>1983)
                     /locus_tag="ACHKUC_005005"
     CDS             complement(1508..>1983)
                     /locus_tag="ACHKUC_005005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621061.1"
                     /translation="ASQTARQTKRSVVCMSAHFVRFPDCQFVDRAEQLWHENGRMICC
                     PVARTARLRGGRRGAKNCRHCASVPVTQTRRNRDRMWECRTAHARSDIPHLVRASPRH
                     PANASRHAKKFRVPLTVLELVKQKIAGPRASGVGSDFVIDDGTGKTLGMVVGVTE"
BASE COUNT          411 a          596 c          539 g          437 t
ORIGIN      
        1 aaaaaaccga gaaacggcgg gaattttcgg gacaggatgt gggaatgtcg gaccgcgcac
       61 gctcgctcag acattcccca cctggtcagg gcttcgcccc gacaccccgc tagcctgatt
      121 cagttttatc tttcgtcggt gagcgacggt actttctgac ctcggttttc cactcgaacg
      181 cgatgcgtga accgtcatcg tcgccctcgg aaatgtcatc cccgatcacc atgtcggcgt
      241 cggtttcctg atcgagtttc agcacgtctt tgagtgcgcc gccggtcgcc acaaagcggc
      301 gcttgtgcgt ctggcgggtc agttcgagga accattcagg atcggccacc atgtcagccg
      361 gcttcgtgga atatttcagg gtttcggcca ctgcgccgcg caccagttca gcggtcgcgc
      421 aggctaagga ctcgccatcc tttggcttac ggggttttac tgcccggaca tcaacattcg
      481 ggttgtaact gacccgcagg cactcccgcc agagttcaac ccagcgagca tgttttacgt
      541 acccgtcacc agatagcatt gagggcggca ccatcatcag ggtgtggaag tggggatgcg
      601 cgctgccgtc gctgccgcgg gtaacttccg ttgtgcggat ccagccctga acaggcagga
      661 actccttgcg aaccttcata cgctgaaaag ctgcattcat ccggttcagc atctcgccca
      721 gttccccaat cgcacagttg cgcacggtca gggtcaggaa catccagcgg gcatcagggt
      781 aatccgccac gatacgcggc agggactggt agaaacgggc ttgccacatc agggagcgcc
      841 tccactggca gacaggacag tgccggacac ggcagaaatg cgcctcacgc agcctcagac
      901 gcgtttctcc ggtttccttg agggtagacc acccgaaacg cagtaaaccg ccacaggagg
      961 ccatacgagc cccgtagcgc tcatattcgg ccgcaagcag gtaaatccct cccacgtcgt
     1021 cagaaacgct tctgtgtgcg tcccagggcg cgtcagaggg ggaataatca gtgaggtact
     1081 ggagtttagg gtttttcatg cgcaccaccc gattgtgggc gatggctcat ctgacgtgtt
     1141 atcattgcca tatcacctgt gttgtcgtgt cactaattcg aatttctaca ggtggtcacg
     1201 cccccgaacg attgccctcg ttgcgggggt cgcttttaac gcgaccactt ccgacagaat
     1261 acttctaaac ccatttcgga gcaaatgtgc cctcaagcct tgcgggacaa gggctggagg
     1321 ccaatttgaa tcctagagtt atcccttaag tataaataca aggatcgcgc gcgagatttg
     1381 cttgaatttt gagctggatt ttcagtaacc caaaacctcc caaccccacc cacttacacc
     1441 catctcatcc acagactttc accccaattt tgtgaacaac aaaaaaccgt cttgcaggtg
     1501 gttggcctca ctccgtgaca ccaaccacca tacccagcgt tttccccgtg ccgtcgtcaa
     1561 tcacaaaatc ggaacccacg ccggaggcgc ggggccccgc gattttctgt ttgacgagtt
     1621 caagaacggt gagcggtacg cggaattttt tcgcgtgccg tgaggcgttg gcggggtgtc
     1681 ggggcgaagc cctgaccagg tggggaatgt ctgagcgagc gtgcgcggtc cgacattccc
     1741 acatcctgtc ccgatttctg cgggtttgtg tcaccggaac actggcgcaa tgtcggcagt
     1801 tttttgcgcc gcgccgccct ccacgtaagc gcgcagttct ggcaaccgga cagcaaatca
     1861 ttctgccgtt ttcgtgccac agctgttccg cgcgatcgac aaactggcag tcggggaatc
     1921 tcacgaagtg cgcactcata caaactacac ttcgtttcgt ttgtcgtgcg gtctgcgacg
     1981 ctc
//
LOCUS       DAZFRG010000441         1974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFRG010000441 DAZFRG010000000
VERSION     DAZFRG010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.443"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..1011)
                     /gene="lpxP"
                     /locus_tag="ACHKUC_005006"
     CDS             complement(91..1011)
                     /gene="lpxP"
                     /locus_tag="ACHKUC_005006"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO6621062.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            1364..1606
                     /gene="yfdY"
                     /locus_tag="ACHKUC_005007"
     CDS             1364..1606
                     /gene="yfdY"
                     /locus_tag="ACHKUC_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO6621063.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(1683..1910)
                     /locus_tag="ACHKUC_005008"
     CDS             complement(1683..1910)
                     /locus_tag="ACHKUC_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621064.1"
                     /translation="MACNVFAFSISGGGSEASYKETEKTSVMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEDITSTYPTKANYLIHPLR"
BASE COUNT          517 a          438 c          477 g          542 t
ORIGIN      
        1 tcagcgaagg aaaaaacctt aagaaaaaag ccataaaaac catgaggtta ttatggctga
       61 tttgaggagg gaaagagtga gcgccgtttg ttaaatgtat aacgacgatt cacccaccgg
      121 gcgcgtttta aagcgacggt ggatccagag gtactgctcc ggtgcgcgca tgatctcttt
      181 ctcgataatc ttgttcatat aggcagcggc ttgattttca tctgtcgggt agccttccat
      241 ctctggggtg atgtacaaac gatatccgct gtaatccgct tttcttacca tcgttacggt
      301 caacatggct gcgccagaga gacgagagag gacataggtg ccattggttg tggcgacatt
      361 ttccaccgca aagaacggcg cgaaggagct gcctttacga ccataatcct gatcgggagc
      421 aaaccatacc gcttcacctt tcttcagtgc actgacaatg ccgcgcagat tatttctgcc
      481 gatcatcgct ttgttagagc gcatgcgacc acgggtctgc acccattcca tcagctgatt
      541 attatgcggg cgataggtgg ccatcattgg ttggcaaagt cccattaccc gaccgcccag
      601 ttccagcgac ataaaatgga cgccgacaac cattacgccg cgattttgca tttgtgcgcg
      661 tttaaggtta tccagccctt ccacatcaaa ccatttacgt acgcgactgt cgggccagaa
      721 ccacgccatg ccggtttcta ccagcgccat gccgagtgaa cgaaagtttt cggcaatcat
      781 cttctcgcgc tctttcgcag aatgctgcgg gaaacaaagt tccaggtttt tacgggcgat
      841 agattcacga cgtttcagga atggtcgcgc cattgtgccg atacgcgtgc cgagaaagca
      901 gagaacagga taaggaagct gtacccaaag ccagagtaca ccaagcccaa accacgtgag
      961 ccagtagcga ggatgcagaa actcgcggga aaatttgcat tttggaaaca tagttacctt
     1021 ctgtagatac gaattgttgt atcgcagaag attccatgtc gacagcggta cactcaattg
     1081 attaggccat catgtacgac aatggttcct gtaaagagtg taaagactag tcaggacata
     1141 accacgagga gcatgaatgc ggtatctgta ctttatcact cgctgactgc tttcagctac
     1201 tgattattaa tcgtgcaaag taaatatttt tccgtacgta acagtcttgc atttaacatg
     1261 ctgttttgat tgatgcttag caaaaattgc cagaggttaa ggattgtctt actattaaag
     1321 tgactgaagt tacaggtttt ttagcgcggc aggaggttga gctatgattt atttatggat
     1381 gtttctcgcc ctgtgtattg tctgcgtaag cggatacatc ggtcaggtac tgaacatggt
     1441 atcggcgatc tcttctttct tcggcatggt gatcctcgct gcactcattt attacttcac
     1501 catgtggtta actggcggta atgaactggt gacggggata tttatgtttc ttgccccggc
     1561 ttgtggcttg atgattcgct ttatggtggg gtatggcagg cgatagtggg tcaggatggc
     1621 tcatttgggc aggtgttcac atcagcatca gacatgattg ttcacctgcc aggtgacaga
     1681 cgctatcgca aaggatgaat gagataattc gcctttgtcg gataagtaga cgtgatgtct
     1741 tctgtgaggt ttaatggtgg tgcatcttct ctcatcttaa acaaaatcgc ctgtgatggc
     1801 tgaagttttg tagaatgcgt tgtcgtcatc actgacgttt tttctgtctc tttatagctc
     1861 gcctcactac caccaccaga aatagaaaat gcaaaaacat tgcatgccat aatagagaca
     1921 aataaaaata agctaaaaag cattaagttt actttcatag aaaatatcct gaaa
//
LOCUS       DAZFRG010000442         1948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFRG010000442 DAZFRG010000000
VERSION     DAZFRG010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.444"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..643
                     /locus_tag="ACHKUC_005009"
     CDS             <1..643
                     /locus_tag="ACHKUC_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFO6621065.1"
                     /translation="HFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPT
                     AYGKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGD
                     IYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHR
                     ITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(609..734)
                     /locus_tag="ACHKUC_005010"
     CDS             complement(609..734)
                     /locus_tag="ACHKUC_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621066.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            754..1911
                     /locus_tag="ACHKUC_005011"
                     /pseudo
     CDS             754..1911
                     /locus_tag="ACHKUC_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
BASE COUNT          574 a          432 c          420 g          522 t
ORIGIN      
        1 tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg
       61 gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca ttgatttttc
      121 tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa
      181 cgatgtgggt gaaattttca tccgtcagtt tgaaagcttt gtcagccgtt ttttggggaa
      241 tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
      301 tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa
      361 taaatcggaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
      421 agcgaaatgt cagcaatgtg tatataaacc tatctgcaat ggtggttgcc ctaagcatcg
      481 tattactaaa gtaaacaatg agactgtttc ctatttttgc gaaggttata aaatcctttt
      541 ttcaaccatg gtaccttata tgaacgccat ggtagagtta gctaagaaca gagtaccgct
      601 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct cccccttagc
      661 tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat tcctgtcggg
      721 tgtttcgtct tcatcatctt cctttgcgaa aatattgccg tatctaataa aggcgattta
      781 agtttccctg cgcagcaaaa atcacttatc gcgctggcta ataaagcagt aagcggaagc
      841 ggcgttggtg aactctcctc ttccagcctg aaacgctgga gtgcggaaaa ctcggtaacc
      901 atgagcagta aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg gactaataac
      961 cctgaacctg gtttccagtt gattaaccag cgaatcaccc acagcacgat taacgacaat
     1021 atttgggcat cgctgcaaaa tgctcaaatt caggcgttga aaacgctcga ccagcgtcca
     1081 gcggagaaat tcgcccagca gatgtatgag acgcgctatg ccgatgaccg cacgaaatta
     1141 ccgcaagaaa atcagattgc acagtttact gccgcagatg cgctggctgc cgatcgccag
     1201 ttgttttcat ctccagcgga tatcacgttt gtcattgtcg gtaatgtcgc agaagacaaa
     1261 ctcgtggcgt taattacgcg ttacttagga tctatcaaac actctgattc gccattagcc
     1321 gcaggtaaac cattaactcg cgcgacggac aacgcatcgg ttactgtaaa agaacaaaat
     1381 gaaccagtgg cacaggtttc acagtggaag cgttataatt cccggacacc tgttaatctg
     1441 gcgacgcgta tggcgctcga tgcttttaac gtcgcactgg caaaagatct acgcattaat
     1501 attcgtgaac aggcatctgg ggcatacagc gtttcttctc gcctctcggt tgatcctcag
     1561 gccaaagata tcagtcattt gctggctttt acttgtcaac cagaacgaca tgatgaactg
     1621 ttaacgttag cgaatgaagt gatggttaag cgtctggcca aagggatcag tgagcaagaa
     1681 ctgaacgaat accagcaaaa cgttcagcgc agcctcgata tccaacagcg tagcgttcaa
     1741 caattagcga acaccattgt aaatagtctt attcaatatg acgatcctgc agcatggatt
     1801 gagcaggagc aattgttgaa acaaatgacg gtagagaatg ttaacactgc tgttaaacaa
     1861 tatctttctc atccggtgaa tacgtatacc ggagtattat tgccaaaata atcacagccc
     1921 cgtcaacaca tcgttggcgg ggatttta
//
LOCUS       DAZFRG010000443         1939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFRG010000443 DAZFRG010000000
VERSION     DAZFRG010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.445"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1207
                     /locus_tag="ACHKUC_005012"
     CDS             2..1207
                     /locus_tag="ACHKUC_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6621067.1"
                     /translation="MRTDVNVLHAAIHRIEWLFETFSSVCLSFSGGKDSTVLLHLTAD
                     VARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVNGVSQFQ
                     PEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRCGVAILT
                     GVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIWIYNART
                     RAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCERVSGAAS
                     GALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVYLRWYQT
                     RGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMK
                     ERRKEWGIL"
     gene            1192..1836
                     /locus_tag="ACHKUC_005013"
     CDS             1192..1836
                     /locus_tag="ACHKUC_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO6621068.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
BASE COUNT          482 a          411 c          549 g          497 t
ORIGIN      
        1 gataagaaca gatgttaatg tattacatgc ggccattcac cgtattgagt ggttgtttga
       61 gacattttca tctgtttgtt tgtctttttc tggaggaaaa gactctactg tgctgctcca
      121 tcttacggcc gatgtggctc gcaggaagaa acgtcgtttc tctgtattat tcattgactg
      181 ggaagctcag taccagtgca caattgcaca cattctgaag atgagggaga tgtatcggga
      241 tgtgacggag actttttact gggtggcact ccccctgact acggtaaacg gtgtctctca
      301 gtttcagccg gaatggatat gctgggaacc aggtgtggag tgggttcgtc agccaccaga
      361 tgacgctatt acagatatgt cgtatttccc attttatcgg tatgccatga cgtttgaaga
      421 atttgttccg gcattttctt cctggtttgc cggtaaccgg tgtggagtgg caatactgac
      481 tggtgttcgt gctgatgaat cgctcaatcg ctttatggga ctggtgtctc agcgcaaact
      541 gagatatgca gatgataaac cctggaccac agcgtcacct gaagggtttt attacacctt
      601 gtatccgttg tatgactgga aagcccgtga tatatggata tataacgcca gaacccgagc
      661 tatctacaat cccctgtatg acctgatgta ccgtgccggc gtgccgttac gcaacatgcg
      721 ggtctgtgag ccttttggcc cggaacagcg taagggactg tggctttacc atgttctgga
      781 gccggaaact tgggccagga tgtgtgagcg ggtgtcgggt gctgccagcg gggcgcttta
      841 tgccaatgag agcggtgcct attttgccct gcgtaagcgt atcacgaagc cgcctcatca
      901 tacctggcgt agctatgcga tgttcctgct ggatgtgatg ccggaaagaa cggcagaaca
      961 ttaccgtaat aaaattgctg tctacctgcg ctggtatcag acgcggggct tcccggatga
     1021 catcccggat gaacaggaga atgacctggg gagccgggat atcccgtctt ggcgacgtat
     1081 ctgtaagaca ctcataaaga atgatttctg gtgccggacc ctctccttca gtccgaacaa
     1141 accccggcac tatgaacgtt atctgcagcg tatgaaagaa aggaggaagg aatgggggat
     1201 tctgtgacac ctgaggtgga agtattgagc aacatgattc gacagtactt cagtcaggaa
     1261 cgatctgaag aagagaccat acgagccctt aatcatctgc gcagggtgtt gcatgaggtc
     1321 agcccttttg cacaggagcc tgtggactgt gtgttgtggg tgaaagcgga tgaggtggtg
     1381 gccaacgatt acaatcccaa tgtcatgtct tccagtgaaa aaaaactgct taaacattca
     1441 ctggagcagg atggattcac gcagcctgtg gtggtttcgg aagagaagga gcattatctg
     1501 gtcgtggatg gttttcatcg tcagttgctg ggcaggaaag cggatacgag aaaacgtctg
     1561 aagggctggc tgccagtgac ctgtattaat ccggaaagaa agggacaggc atcacgtatt
     1621 gccgcaacca tacggcataa ccgtgccagg ggaaaacacc agataacgtc aatgtctgac
     1681 attgtcaggg acctttcgcg tctgggctgg acagatgagc gtattggcac agagttgggc
     1741 atggaccagg atgaagtgct gaggctgaaa cagattagcg ggctgacaga gctgtttcag
     1801 gaagagaatt tcagcccttc atggacggtt cgttgatatt gtaaaatatg atagttggag
     1861 taaggggcaa tagaaggaga atatttatcc caccttcttc cgagtcggga taaatcaaaa
     1921 ggtattatcc attgattca
//
LOCUS       DAZFRG010000444         1928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFRG010000444 DAZFRG010000000
VERSION     DAZFRG010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.446"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(357..950)
                     /gene="tehB"
                     /locus_tag="ACHKUC_005014"
     CDS             complement(357..950)
                     /gene="tehB"
                     /locus_tag="ACHKUC_005014"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO6621069.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNTMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(947..>1928)
                     /gene="tehA"
                     /locus_tag="ACHKUC_005015"
     CDS             complement(947..>1928)
                     /gene="tehA"
                     /locus_tag="ACHKUC_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO6621070.1"
                     /translation="KVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGLVILA
                     MIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAV
                     CLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDA
                     GLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGE
                     GDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSDN
                     GFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
BASE COUNT          551 a          509 c          429 g          439 t
ORIGIN      
        1 cagagccaac aagccgcata aagctgcaac tttcgcaaat gatgtggtac gcatgaatag
       61 tttcctgaga tgattatatt actttctaag ttatagcaaa aagcgccgct tcgtgttgtg
      121 gcaaaaaaag tgttggtgaa aataaatttt ttagcccctt ttcagacgac atagtcaatg
      181 ccgaaaaagg ggctaagtca atcatcagta gttaaatttc agtaagttaa gcggctaaaa
      241 aacttcgact actgtgatta atttcacatc ccttcgataa aaaatatgct taccgaaaag
      301 aagaacagta aaccatctca gcgtggggaa ttttcacgcg ctttgttctt accggatcat
      361 tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac gattaccgtt ggcgtcggtg
      421 cggtgcagct cgccgacgtc ttcattgtat ttcaccctct cccagccttc gtaatatcga
      481 cgtaattccc cctctttgaa ggcaaacggg aagccgacgg tacatggata atcagcggta
      541 tccatcgccg ccacaatcag gttgtaaccg ccaggtttag tgcaacgttg catattggca
      601 atcagccctg ggatggtttt agcctcaagg aacatcagca ccacagtcga aagaataaaa
      661 tcgtactgtc catcaaatgt gaggttattc agatcaacga cccgggtgtg taaattatcc
      721 agattttcaa tggacttaat gcgctcgacg ttggcgatac tcatggtatt tttatcccat
      781 gcgtcaacat cataaccatt ggctgccagg taaagactgt tacgaccatt gccacagccc
      841 agatccagcg ttttacccgg tttaaccact ttcaccgctt ccagcacttc agagtgtgtg
      901 cgggttaatt catatttatc agtaaaatag ttttcatcac gaatgatcat tctttgtcct
      961 ctgctttcat taaaacggcg cgctcggttc tgactaacaa ttttccctgc atcagaagcg
     1021 caaaagtacg gatgagcagt attgcaataa taaaattggt aaagataaac agcggcaccg
     1081 ccagcgtatg gaaaaatcca ttatcgctgc cactccccag atgcaaaccg gtggttgcca
     1141 gtgcagatac accgaacgag aaactccaga atgaagcatt aaatggctgg gagagatacc
     1201 atggcatcag acgtagcata aacagcagtt gcagcagtcc ataaccgaaa agcattttcg
     1261 ccagcgtgtc accctcgccg ccgttgacgc tcagccaggc actacaggcc accagcgcag
     1321 gagcgagctg aatgccgagt gatgtccgca gtgccgtggg taattctccc gaactgcgca
     1381 gacgctgcaa gatcaccggt tccaggctta gccatgagaa aacgcctgcg cctaaaaaca
     1441 ccagaccggc gtcggtgtag cccaacgcac cacaggccat tgcgctgata aagttgttgg
     1501 caactgtcgg cagatacagt ccaggcgtgg tagcttcttc agggtgagat ccgcgccata
     1561 atcccgcagt ttgccaggcg gcataagcca actgaaccac gacaccaaaa ctgaacaggc
     1621 acaccgccag tgggcgaaac cacggaacaa aaccaatcgc caccagcatc gtcgttgccg
     1681 gaaacaaact cacaaaactg ctcagcactg gatggcgcac ttctgccagc acgctatgcg
     1741 gaaagcgtat gagtcgggta ataaatgcgc cagtcaataa tccccagatg atcatcgcca
     1801 gaatcaccag cccatccccc aaccagtggc tgacctgcca aacctggctg gcatagcgcc
     1861 aggcaaatcc catccctatc gtccccaaca caataccaaa gtagcctgcc ggcaaattga
     1921 gcacttta
//
LOCUS       DAZFRG010000445         1870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFRG010000445 DAZFRG010000000
VERSION     DAZFRG010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.447"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            211..405
                     /locus_tag="ACHKUC_005016"
     CDS             211..405
                     /locus_tag="ACHKUC_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621071.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHNHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            413..1825
                     /gene="pykF"
                     /locus_tag="ACHKUC_005017"
     CDS             413..1825
                     /gene="pykF"
                     /locus_tag="ACHKUC_005017"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO6621072.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          505 a          466 c          437 g          462 t
ORIGIN      
        1 tttcacttat cctcacactg acaaccccgg caccagacgt tgcgcaaaca acgaagtttt
       61 tgcgtaacct tttccctgga acgctgaatc tttgataaca atttattgtc ttacaagttg
      121 tatatttttt gaaacgctgt ttttgttttc cttttggatt aatttcagcg tataatgcgc
      181 gccaattgac tcttgaatgg tttcagcact ttggactgta gaactcaacg actcaaaaac
      241 aggcactcac gttgggctga gacacaagca cacattcctc tgcacgcttt ttcgatgtca
      301 cctatcctta gagcgaggca caaccacttt cgtaataccg gattcgcttt ccggcagtgc
      361 gcccagaaag caagtttctc ccatccttct caacttaaag actaagactg tcatgaaaaa
      421 gaccaaaatt gtttgcacca tcggaccgaa aaccgaatct gaagagatgt tagctaaaat
      481 gctggacgct ggcatgaacg ttatgcgtct gaacttctct catggtgact atgcagaaca
      541 cggtcagcgc attcagaatc tgcgcaacgt gatgagcaaa actggtaaaa ccgccgctat
      601 cctgcttgat accaaaggtc cagaaatccg caccatgaaa ctggaaggcg gtaacgacgt
      661 ttctctgaaa gcgggtcaga cctttacttt cactactgat aaatctgtta tcggcaacag
      721 cgaaatggtt gcggtaacgt atgaaggttt cactactgac ctgtctgttg gcaacaccgt
      781 actggttgac gacggtctga tcggtatgga agttaccgcc attgaaggta acaaagttat
      841 ctgtaaagtg ctgaacaacg gcgacctggg cgaaaacaaa ggtgtgaacc tgcctggcgt
      901 ttccattgcc ctgccagcac tggctgaaaa agacaaacag gacctgatct ttggttgcga
      961 acaaggcgta gactttgttg ctgcttcctt tattcgtaag cgttctgacg ttatcgaaat
     1021 ccgtgagcac ctgaaagcgc acggcggcga aaacatccac atcatctcca aaatcgaaaa
     1081 ccaggaaggc ctcaacaact tcgacgaaat cctcgaagcc tctgacggca tcatggttgc
     1141 gcgtggcgac ctgggtgtag aaatcccggt agaagaagtt atcttcgccc agaagatgat
     1201 gatcgaaaaa tgtatccgtg cacgtaaagt cgttatcact gcgacccaga tgctggattc
     1261 catgatcaaa aacccacgcc cgactcgcgc agaagccggt gacgttgcaa acgccatcct
     1321 cgacggtact gacgcagtga tgctgtctgg tgaatccgca aaaggtaaat acccgctgga
     1381 agcggtttct atcatggcga ccatctgcga acgtaccgac cgcgtgatga acagccgtct
     1441 cgagttcaac aatgacaacc gtaaactgcg tattaccgaa gcggtatgcc gtggtgccgt
     1501 tgaaactgct gaaaaactgg atgctccact gatcgtggtt gctactcagg gcggtaaatc
     1561 tgctcgcgca gtacgtaaat acttcccgga tgccaccatc ctggcactga ccaccaacga
     1621 aaaaacggct caccagttag tactgagcaa aggtgttgtg ccgcagctgg ttaaagagat
     1681 cacatctact gatgatttct accgtctggg taaagaactg gctctgcaga gcggtctggc
     1741 acacaaaggt gacgtcgtag ttatggtttc tggtgcactg gtaccgagcg gcactactaa
     1801 caccgcatct gttcacgtcc tgtaatattg cttttgtgaa ttaatttgta tatcgaagcg
     1861 ccctgatggg
//
LOCUS       DAZFRG010000446         1842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFRG010000446 DAZFRG010000000
VERSION     DAZFRG010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.448"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHKUC_005018"
     CDS             complement(<1..266)
                     /locus_tag="ACHKUC_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-cystathionase"
                     /protein_id="HFO6621073.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPE"
     gene            complement(266..>1842)
                     /locus_tag="ACHKUC_005019"
     CDS             complement(266..>1842)
                     /locus_tag="ACHKUC_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO6621074.1"
                     /translation="QKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLI
                     PFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMA
                     MHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKIVN
                     LRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYF
                     FYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLD
                     LAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFL
                     FLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCV
                     EVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQ
                     MVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVIIGT
                     KVIALRRDMDNYMGIY"
BASE COUNT          573 a          445 c          356 g          468 t
ORIGIN      
        1 cattcaggtt taatatctgt ttgatattgt tgataatacc agtttactat cgcattcaga
       61 tattcaccat gttgccaacg actgtaccct aaaatcgggt gttccaggcg tttttttaat
      121 gctctggtga tagcgtctgg tatcggaaga tccatatctg aaatagagaa tggtagtaca
      181 tcttttacac caaatctgtc ctcgcaaaag tcccattggg tacaatatgt tcctttgcgg
      241 gaaacaggag tattaaaaac atccatcagt atatccccat atagttatcc atatcccgac
      301 gcagggcgat gactttagtg ccgataatga cttgtagggt attttgatca agtttaacaa
      361 ccgcaatccc gccagctctt tttattgcag cctcgtcaat caatcccata tctgcgattg
      421 ttaaacgtaa acgagttacg caattatcca gtgacttaat attctcttta ccaccaagtg
      481 ccttaaccat ctgctccgcc gcttgcagct ctgatttatc aggagttggg atatccgcta
      541 tagccgtatc ttcgtgagcg ttcagcgtga caggactgga ctcttcacgg cctacggtca
      601 tcaggttaaa tttaagaatg gtccaacgga acacaaagta ataaatgaag aaataggcca
      661 cttcgacaca taccgcgatc ggccaacctg ttcttgtgcc acgcaatacg ccaaagctaa
      721 taaattgaat cagatcacca gtaaatccca tcttcacgcc catatatggc agaaccatgt
      781 tagcgaatcc attcataaag gcatggaaga caaacagcag tggactgaca aataagaaca
      841 gaaactcaat cggttcgctg acgccgccaa taatcaccgt aagcaccccg gaaataagca
      901 atgctttgat agcctttctg ttttgagcat gagcagtgcg atacatcgcc agcgcggcac
      961 cagccagacc atactgaatc atcagtttgc cctgctccat tttccccgcc aaatccagcg
     1021 gaatgaccgc attctctttg acgtatgcca taaacatgtt cagggtgccg taatattctt
     1081 caccgccaat cacagccgaa ccgccgattg gcgtaaagcg gaacactgac gtcaccagat
     1141 ggtttaagcc aaaaggaatc gtcacacgtt cggcaactgc atagaagaaa taaccaacag
     1201 gaccggaagt tgatatccag tggccaatga gcatgaagag attgaaaaat ggcggccaga
     1261 taaaaggaat gatcagacca aatccactca tcacaatcag tgtaatgata ggcaccagac
     1321 gtgggccgct ataaaaacct aatgattctg gaatgcgtaa attaacgatc tttttataca
     1381 tgctggcgac taataaccca gcaacaattc cccccaacac gctggtattg taggactgga
     1441 tccccagaat gatggtttgc ccatgtgtcg acatttggtc agcaacgacc aataagtcgt
     1501 gctgtttaag ataaaagttc gttcccagat gcatcgccat aaaaccaatt aagccagaaa
     1561 aagcaccata ggctttatcc tctttatctt ttaataatcc taagggaatc gctatcgcaa
     1621 acaatacagg taaattaaca aaggcaaaca aaccaagact aacaatgaaa tcaagtatgg
     1681 ttttaattat tggaatagcc agaaatggaa ttaactttgc catatcatca ctggctaaac
     1741 cacttcccag ccctagcatc atgccacata cacttagcag agcaatggga tacataaatg
     1801 ccttccccag gctctgaaaa aaactccagg ctttcttttg tt
//
LOCUS       DAZFRG010000447         1841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFRG010000447 DAZFRG010000000
VERSION     DAZFRG010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.449"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..717)
                     /locus_tag="ACHKUC_005020"
     CDS             complement(<1..717)
                     /locus_tag="ACHKUC_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjP family GTPase"
                     /protein_id="HFO6621075.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSHICATHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPEVDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQALSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRS"
     gene            902..>1841
                     /locus_tag="ACHKUC_005021"
     CDS             902..>1841
                     /locus_tag="ACHKUC_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621076.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLAELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRGDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYPANSSGVSGYSDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPIFGLLFYEQERLTGGVFRYVLIKF
                     YNAFKENLFFVEIVSCCFLVEFFHDIDSFFINQRLSK"
BASE COUNT          559 a          325 c          455 g          502 t
ORIGIN      
        1 agagcggttt tctccccgga tatgtgaata caccgcgctg gttgcctgtg gtggcagtgc
       61 gtggatcatc agcgacacaa acgccggaag gttccagccg acaggtgcag caacggagag
      121 taccggaaat gatgaaggga acagggttgc cacccgggct gtcaccgtcg ccagtgagag
      181 cgcctgctga cgggacgggc atttttctgt ggcattccat tcttcagcag gaaacacgcg
      241 atcggcatgg ctgagaacaa acagaaaacg cgagggatct gcgccttcat tcagtaaaaa
      301 ctgatgcatg gtaatgtcgg cggcatacgc gcgttcatct gcccgcagga tccagataat
      361 caaatccact tccggcagta actgacggta aagcgcccgg tattcctgat catgttgcgg
      421 cgtctctcca atgccgggca gatcgaccag cgtcattctg cgttcaccga tctgcagggt
      481 aagacgatga gcctggcggg tgcagccgtt caggggatgt gtggcacaga tatgggactg
      541 aaaaatagca ttacacaggc tacttttgcc ggtgccggat ttacccataa tgccaatcac
      601 gggttcgtaa tgaataacgc tgtggagatg ttcaagaatt ttctgacgaa tttcttcagg
      661 aagaaacgtc agatatgatt tcacttgtgc ggagagggaa gaatggctga atgacatacc
      721 ggtatcctgt tgatttcact gaaccggatc acactaatcc ggtttatcat tcaggtaata
      781 tggacgtgaa aaatgatgga atgtctgaat tacggacatt attgttaaag tgtaatggag
      841 tatgaagtga gtttttcaga acgggaacta atgacatact gcggtttcag gagatgagag
      901 gatgaccgga cgtaaaaaca aatgcgtcag aaaaaattgt aacaatcagg ttttctcagt
      961 aaaaatggca gtggagaaaa gactgtgcag aaaacattat gagaggtttt gtgagctcaa
     1021 aaaaagaaga gagcaaaatc ctgttcacag attaaatgaa gcattgccaa gaaagataaa
     1081 aggacccaaa agtaagaaaa aattagtaaa agaaagagaa atcattaaca gcaggaagaa
     1141 acataaacag gagcagggac catcgctggc tgaacttaat aaaaaagttc gtgagatact
     1201 gaaaactccg gcatggagaa cgctggaaac gagaattcga agaaacggta tcacatttaa
     1261 atcaaaagca gagttagatg tactttctga actttatgca atttattcat gggtttcata
     1321 ttgcaaacgg ggcgataatt attctgtgtg gtttttgaat tgtccggatg taaatataaa
     1381 tcttcatgtg tgcgaatgca ggtcttctga aagcgattgt tatctaccat cccgttttat
     1441 tatccttcca gctccattaa ggcaaaaagt aaaaagaaaa taccctgcta attcgtctgg
     1501 agtgtcagga tatagtgatt caaaaagtct ttataagact gttttgtctg tttgtggtgg
     1561 gaaaagagaa accgaattgt tcttttattc actgccagta aagactaatc atcatcctta
     1621 ctggattaaa agtggtgtgg ttaaaagatt tccgcctatt tttggcttgt tgttttatga
     1681 acaggaaagg ttaacaggag gtgtttttcg ttatgtgttg ataaagtttt ataatgcttt
     1741 taaggagaat ttgttttttg ttgagattgt ctcgtgctgt tttcttgttg agttttttca
     1801 tgatattgat tcatttttta taaatcaacg attaagtaag t
//
LOCUS       DAZFRG010000448         1836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFRG010000448 DAZFRG010000000
VERSION     DAZFRG010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.450"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(148..801)
                     /gene="ribB"
                     /locus_tag="ACHKUC_005022"
     CDS             complement(148..801)
                     /gene="ribB"
                     /locus_tag="ACHKUC_005022"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO6621077.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(905..1053)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1175..1474
                     /gene="ubiK"
                     /locus_tag="ACHKUC_005023"
     CDS             1175..1474
                     /gene="ubiK"
                     /locus_tag="ACHKUC_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO6621078.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPIAELQSPPAIPPVDKAE"
     gene            complement(1509..1709)
                     /gene="glgS"
                     /locus_tag="ACHKUC_005024"
     CDS             complement(1509..1709)
                     /gene="glgS"
                     /locus_tag="ACHKUC_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO6621079.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT          513 a          476 c          411 g          436 t
ORIGIN      
        1 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
       61 aacatcttgc atagcaggga aaaataaaaa ccgaagcctg ctacaacttc ggtttcttga
      121 ttaaggcagt aaattaagca gcggttttca gctggcttta cgctcatgtg cctgacggta
      181 tgccaccagg tcttcaatag tcacgagcgc catattgtgt ttattggcaa actcaatgca
      241 ctctggtgca cgcgccatcg tgccatcgtc attagtcagc tcacacagca caccagctgg
      301 tttaaagcct gccagcgtca tcagatcgat agtcgcttca gtatgaccgc cacgcgtcag
      361 tacaccacct gcctgcgcgc gaagtgggaa aacgtggccc ggacgattca gatctgaagg
      421 ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata cggtcagcgg cagaaacacc
      481 ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa ccggtgccat aggcgctggt
      541 gttattttct accatcattg gcagatcgag ttgtttacgg cgatcgtcag taatgcacag
      601 gcaaacaata ccactcccgt ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc
      661 tgccgggaag atcatatcgc cttcgttttc acggtcttca tcatcaagca ccatcacacc
      721 gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga
      781 ggaaagtagc gtctgattca tggtaaaaaa acctcactaa aattatggtt accagaatca
      841 gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc atgcccgaca
      901 gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac accggatctg
      961 ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg atttaccgcc
     1021 ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct aatgattagc
     1081 gccagcaacg catagcttca cataattctg gtttatgact tacccttatc gcactacaat
     1141 ggcactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa aaattgagca
     1201 aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg gggaagatgt
     1261 ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg cgtctcgatc tggtaagccg
     1321 cgaagaattc gacgttcaga cgcaagtcct gttacggact cgtgaaaaac tggcgttact
     1381 ggagcagcgc atcagcgaac tggaagcgcg caataatccg atagcagaac ttcaatctcc
     1441 tccggctatc ccgccagttg ataaagcaga ataaagcacc acaaaaacgg gccagacggc
     1501 ccgtaatatc agtgttctaa ttcaagttct ctggtttgca tcatctgttg gcaataccag
     1561 gcataacgcg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
     1621 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgcgag aaaatcgaaa
     1681 ttatttaaag aattaagact atgatccata agcacatcct ccggttattg catacctttt
     1741 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
     1801 acgtgcgtaa atatattgtc cccgatcaca cttttt
//
LOCUS       DAZFRG010000449         1827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFRG010000449 DAZFRG010000000
VERSION     DAZFRG010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.451"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..643
                     /locus_tag="ACHKUC_005025"
                     /pseudo
     CDS             77..643
                     /locus_tag="ACHKUC_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            890..1462
                     /locus_tag="ACHKUC_005026"
     CDS             890..1462
                     /locus_tag="ACHKUC_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621080.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKIIGILERALREQSGSRIRRILLR
                     LPCEYDRTFSSRKIWFGMDFIETVSALMNVTSGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGSELDRLILDAWSNVLGFMSPGEAAPYQKQITRDVVLDSRSPDCEEIFQALIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQL"
     gene            1531..1767
                     /locus_tag="ACHKUC_005027"
     CDS             1531..1767
                     /locus_tag="ACHKUC_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6621081.1"
                     /translation="MLTSITGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
BASE COUNT          452 a          460 c          440 g          475 t
ORIGIN      
        1 ttagatctga ttattagcga aagtattaat tcgcttacag cacttaaaac gtcattaact
       61 ttcaggagtc atcaacatgc ttaaatctta cacaccaaac tggtttttta ccgctttact
      121 tgacaatcac atcaatcaaa tgatggcacg ctattcctgc ctgcgggcct tacggatgga
      181 tttcttctac aggaaggata cgcctgattt cttacaacct gatcatcgtt ggcttgaatt
      241 acaactgcgt atgctgctgg agcaggttga acagtttgaa aatatggttg gcttcttctg
      301 ggtgattgaa tggacggttg atcatggttt tcatgcgcat gtggtcttct ggctcgatcg
      361 tcagagggtt aaaaaaatat atccctttgc ggagcggatt acggaatgct ggcggtctat
      421 tacgcataac agcggttcgg cacaccgctg tacatatcag ccgcattata catacaacat
      481 caacattcct gtgcaccaca acgatcctga aagcatcgat aatattcgcg gtgccctgca
      541 ttatctggca aaagaagagt aaaaagacgg gctgtgtgcc tacggctgca atgaagttcc
      601 tgaacgtcct gctgcagggc gtcctcgtaa gcctcacttc tgaagcttaa ggtctgagcc
      661 tccgctcctg gaaacactcc gtcggtaaaa acttaccgcc ttgattaatg atgtgaaccg
      721 aagtcaacgg agatcattcc ccctgaacct gcatccggtg ttctttccct tatcttcccg
      781 ttctgcttca gttcttcact tattccatca atctcatccg caagccataa cacgtcagct
      841 cattcacggg caggacgcat tgtgggctgc gcataacgga acatatctta tgaatgctat
      901 tccttatttc gactatagcc tggcaccctt ctggccgtct tatcagaaca aaatcatcgg
      961 tatccttgag cgtgcgctgc gtgagcagtc cggctcccgg atacgacgga tcctgcttcg
     1021 tctgccatgt gaatatgacc gtaccttcag cagcagaaag atctggttcg gtatggactt
     1081 tatcgaaacc gtcagtgcgc tgatgaatgt aacctccgga cgtgatctct gctggctcct
     1141 gactcgtcat cctgaaaagc cggaatacca cgtggtgctg tgcgtcagac aggagtattt
     1201 cgatggctct gaactggatc ggctgattct tgatgcctgg agcaacgtac tgggcttcat
     1261 gtcaccgggt gaagcggcac cgtaccagaa acagataacc cgggatgtgg tgctggacag
     1321 tcgttcaccg gactgcgaag agatatttca agcgctgatc tgggcgttca gtgatttcgc
     1381 ccgcgatcgt cgtggtgtac gcgatccgga agcccgttac cttgccggta atccctggta
     1441 tccggtagca ggccagctct gaaagctcag ttcagcagat tcacccgcat ttactcaacc
     1501 ttcataaatc tcacagagga tatcctgact atgttgacct caataacagg ccacgacagt
     1561 gtattgctgc gtgccgacga tcccctgatc gacatgaact acatcaccag tttcactggc
     1621 atgacagata aatggtttta caagctgatc agtgaaggtc atttccctaa acccatcaaa
     1681 ctggggcgca gcagccgctg gtacaaaagt gaagtggagc agtggatgca gcaacgaatc
     1741 gaggaatccc ggggggcagc agcatgaaac gtgttgtaat gccggtacgc tggcaatgtg
     1801 caaaatgcca gcgctggtat tgtggaa
//
LOCUS       DAZFRG010000450         1817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFRG010000450 DAZFRG010000000
VERSION     DAZFRG010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.452"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..714
                     /locus_tag="ACHKUC_005028"
     CDS             join(17..266,266..714)
                     /locus_tag="ACHKUC_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFO6621082.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMGEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            986..1537
                     /gene="papX"
                     /locus_tag="ACHKUC_005029"
     CDS             986..1537
                     /gene="papX"
                     /locus_tag="ACHKUC_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HFO6621083.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT          518 a          402 c          439 g          458 t
ORIGIN      
        1 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
       61 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
      121 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
      181 aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt
      241 tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
      301 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg gcgaacagtg gggctatgtc
      361 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acaggctccg gaagacggtt
      421 gttgcgcacg tattcggtga acgcacgatg gcgacgctgg ggcgtcttat gagcctgctg
      481 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
      541 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
      601 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
      661 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
      721 gtcattacct acaacgttta tcgacctcac acgaacaacg atggtttacc gccttgctta
      781 catgaagaaa agtggaaaca ggttattccg gtgtcctgat tttgtgaacc actacaataa
      841 tcctttttca cagacttgtc agcagccagc atttatgttc ttttacctgc gggaatttat
      901 ccgtctgcct gtcgggacta ctctgtcata cagaaatcag gtaggaataa attgtagtgg
      961 aaagtcgatg tttaccggat gactgatgcg cgcttgtaca cagacagtgt gtttcagtaa
     1021 tatggagaat aatgaaatga ataacacaga cacattagaa aaaataatca gacaccaaaa
     1081 aaacaaagac cccgcatatc cctttcggga acatttgttg atgcagctct gtattcgcac
     1141 aaataaaaga atgcaggata atatatctga atttctgggg gtgtatggaa taaatcactc
     1201 agcatatatg gttctcacca cattattcgc agcggagaac cattgtctgt caccttcaga
     1261 gataagccag aaacttcagt ttaccagaac taatattacc cgcattacag attttttaga
     1321 aaaagccgga tatgtaaaaa ggacggatag cagggaggat cgccgtgcta aaaaaatcag
     1381 tctgacatct gaaggtatgt tttttattca gaggctcact cttgcacaaa gcatgtatct
     1441 gaaagaaatc tgggattatc tgacccatga tgaacaggaa ctgtttgaag tcattaataa
     1501 aaagttactg gcacattttt ctgatgccag ctcataaagt gcgaaatatc tgaggatgct
     1561 ggatagcttc aggcaagaga ataatgattc ttgcagatgt gtttttccgg atacaaaaac
     1621 aaaagtaaaa aattgcagcg ccaggccacc tttcaaagca gggaggcctg taccgcgtcg
     1681 aaaatttcag ccagttaatg taattgtctg aaccaggcac ttcgcccggg caggagaagg
     1741 agcgtggttg cggtcatcct gtatactgaa gtcgttatga aggaataata caaataattg
     1801 cccgttttct ctgtacg
//
LOCUS       DAZFRG010000451         1800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFRG010000451 DAZFRG010000000
VERSION     DAZFRG010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.453"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..506)
                     /locus_tag="ACHKUC_005030"
     CDS             complement(21..506)
                     /locus_tag="ACHKUC_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6621084.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(561..1379)
                     /locus_tag="ACHKUC_005031"
     CDS             complement(561..1379)
                     /locus_tag="ACHKUC_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6621085.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(1480..1713)
                     /locus_tag="ACHKUC_005032"
     CDS             complement(1480..1713)
                     /locus_tag="ACHKUC_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6621086.1"
                     /translation="MLGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEADAGKQLNAWLASGGLLRQ"
BASE COUNT          407 a          490 c          482 g          421 t
ORIGIN      
        1 tgcattgtcc ctgcctccat tcagtcgatg atgcgcataa tggcgctgca ttccggatgc
       61 tgcagggcgt aatcccgcag ccggtaataa tggaccgtca tggcataaca ctccgtacga
      121 caggcgtgat gactgtacgt catcagacag gcagcaatgc cagcggcttc cgggctcatt
      181 tcagcgcggt tgccgttcat ggcattgaac agtacccatg tttcgtcatc atcgtcatcc
      241 ggttcggggg ccataaatgc cccgccgttg ttcagggtgt acagattcca gataccaccg
      301 cagtagtctt cgcacagacg gtccatccag ccgaaaacac ggggctccag ggccacccac
      361 tgtggaatga ggccaaaatg ctgcggccag aagctgatgc gctgttcatc agggactatg
      421 gtggcaacca actgaggctg gtcattccct gatgcagcgg ttacggaaac agaagatgtg
      481 gtggaattat gtgaaatggt tttcatgaaa tgactcctta aaggacaaat aaatgcgtgg
      541 ttttcagggt aatggctgaa tcagcgcagg ctctccagca gggtttccgc catcacccac
      601 aatgcgcggt tgagcttaat gtcagtgtca atgctgtgaa tggcacgggt atggatacgt
      661 tttcctttag cgctgcgacc tgaaattccg cctttcagca tattctcctg aatggtctga
      721 taagcactcc acaggtcctt accgtaatcc tcccggcgtc gtggcgtcag aatgtcggcg
      781 gtggtgacgg gctgatgttc gtcaccataa cggtaagtca gtgccgcctg tgccagcgcc
      841 tggcgtgccg gtggcggcag aatcagcgac tgcatggcat cacgcttttc ctcaatccgg
      901 tcaaacacgc ccactacctc gtaagcccct tcaatgactc tgtccaccac atttccccgg
      961 tgcggaacac gcacttcccc cagagactga ccacagacgc acccgttctg gcagacgaac
     1021 ctgaagtaac ccggcagcat ctggtagctg gaggtaccgt catgagagtt gagcagaata
     1081 atttcaggga catgttctcc gtttatctct ccggcccgcc gcagacgcag catgtgtttt
     1141 gtgtatcccc ggcggcccgg gtcgcgcaca cgggtctggc aggcgaagaa tggctgaaag
     1201 ccttcccgct gcaggctttc cagtacggtg atggtgggga tgtacgtata gttctggctt
     1261 cgggacgtgt gtttatcttc cccgaaaatc cccggcacat agtgcatcag ttcttcgtgt
     1321 gtcagcggac ggtcacggcg tatctggttc gcataaccaa aacgactggc tagtcgcata
     1381 atttgctcct tatcggtggt taagatttac tggtgtaata aatgaaaaaa gccgcgtctc
     1441 ccggagaaga cgcggcctga tggatgaaat caatgaggtt tattgtctga gaagccctcc
     1501 actggcgagc cacgcattaa gctgttttcc ggcatcagct tcagagttcc ggcagcgcca
     1561 gcgtcgctga ccttccgcgt tgcggataac ccgacggaaa tgcattcccg ggtcatcctg
     1621 gataaacaca ttgcggtaag cggttatgac agcaaccgcc tgcccgtatg agaaagatcc
     1681 ttcagccagg acatactctg tgtaacccag catatctgtt ttctcctgat aaataaaaag
     1741 cctctgccac gcgttgtgac agaggctgaa gcatgaaact gacttcaggg atcagttaac
//
LOCUS       DAZFRG010000452         1766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFRG010000452 DAZFRG010000000
VERSION     DAZFRG010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.454"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..833
                     /gene="rcnA"
                     /locus_tag="ACHKUC_005033"
     CDS             9..833
                     /gene="rcnA"
                     /locus_tag="ACHKUC_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO6621087.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1052..1390
                     /gene="rcnB"
                     /locus_tag="ACHKUC_005034"
     CDS             1052..1390
                     /gene="rcnB"
                     /locus_tag="ACHKUC_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO6621088.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1472..>1766)
                     /locus_tag="ACHKUC_005035"
     CDS             complement(1472..>1766)
                     /locus_tag="ACHKUC_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621089.1"
                     /translation="QVYLSSSDLLTNSNTILKGAEDNGVGFILESNGTPVTLLNITNS
                     SKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT          435 a          425 c          411 g          495 t
ORIGIN      
        1 ttattatcat gaccgaattt acaacacttc ttcagcaagg aaacgcctgg ttcttcatcc
       61 ccagcgccat cttacttggt gcgcttcatg gcctggaacc ggggcactca aaaacgatga
      121 tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca ggcggtgatg ctcggactgg
      181 cagcaaccat ttcgcatacc gcagtggtct ggttaattgc ctttggcggg atggtgatca
      241 gcaaacgctt tactgctcaa tcagcagaac cgtggctcca gttgatttcc gcagtgatca
      301 ttattagcac cgcgttctgg atgttctggc gtacctggcg cggcgaacgc aactggctgg
      361 agaatatgca cgagcatgat catgagcatc atcaccacga tcacgaagat caccacgacc
      421 atggacatca tcaccatcac gaacatggcg agtatcagga tgcccatgca cgagcccatg
      481 ccaatgacat taaacgacgc tttgatggta gagaggtcac caactggcaa attttgctat
      541 ttggcttaac cggtggcctt atcccctgcc cggcagcaat taccgtgctg ttgatttgca
      601 ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt cagtttcagc ctcggcctgg
      661 cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag cgttcagcag gtcgcaaaac
      721 gctggagcgg atttaacact ctcgctaaac gcgctcccta tttttccagt ctgttgattg
      781 gcttagttgg tgtgtatatg ggcgtacatg gcttcatggg cataatgcga taactcaatc
      841 ctttaggctt gcatgttatc ttcagcccag gatgattctt aaatcagcta tttccgctga
      901 ctctctgctc gctaaggatg cttttagggc atcctttcat ttacactttt tacgaaatca
      961 tgggatcact aacaaaatat cgcttgtcag ttatattgta tggcaggaaa gatatgcgac
     1021 tgatattaca gatccccaaa gtggagagtt tatgaccatt aaaaataaga tgttgctggg
     1081 tgcgcttttg ctggttacca gtgccgcctg ggccgcacca gccaccgcgg gttcgaccaa
     1141 tacctcggga atttctaagt atgagttaag cagtttcatt gctgacttta agcatttcaa
     1201 accaggtgac accgtcccag agatgtaccg caccgatgag tacaacatta agcagtggca
     1261 gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg acctatatgg gtggcgcgta
     1321 cgtgttgatc agcgacaccg acggtaaaat cattaaagcc tacgacggtg agatttttta
     1381 tcatcgctaa aaaaaagccc cctcggagag aggggggaat gcagacacct taatattttt
     1441 attattagcc acttgctcgt cttgcttgtt attagtcgta tttcacgttg attaatgcgg
     1501 ttgcctccag tgcgccagat ttaactttgt ttgtatcgta gacgtagtaa ctggccgtta
     1561 tcggaattga aaccgttgta tctgtaagtt ttgactttgc cgcaatttct tttaaatttg
     1621 tatatccttt actgctgtta gtgatattta aaagtgtgac tggcgtacca ttactttcaa
     1681 gaataaatcc tacgccatta tcttccgcac ctttcaggat cgtgttgctg ttggttaaca
     1741 agtcagaact tgataaatac acctgc
//
LOCUS       DAZFRG010000453         1739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFRG010000453 DAZFRG010000000
VERSION     DAZFRG010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.455"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..>1739)
                     /locus_tag="ACHKUC_005036"
     CDS             complement(121..>1739)
                     /locus_tag="ACHKUC_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6621090.1"
                     /translation="VVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQPIFGLHVEFNPEL
                     FLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGYVIHLLLPNVPLI
                     AAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGLVALKFAVAVTMG
                     TMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEPSIQIVLMLLLPF
                     IVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWSMLEYVFNGMVFL
                     ILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGWLWLMRLYSRKRP
                     GKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFPGRYLLVFLATGV
                     ILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSVQKTEQRLLRART
                     ENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNLAALNAKRAAIYQ
                     MRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT          508 a          452 c          379 g          400 t
ORIGIN      
        1 attaatgcag tattaatcca ggcgattgtt ctgcccataa ggaaatacat agggaataaa
       61 cgatgctgga agtattactc tcagccatca tttaattccc tattactggc agcgataaaa
      121 ttatcggcta tccatgatta acgtttccag cacatctaat tcactgacta tttttgccag
      181 tgtttcatca ctgacttctt gccttgcgcg catctggtaa atagccgcgc gtttggcatt
      241 taacgccgcc agattaaatg ttctttccag cttcgcacgc tgcatatgca gggaattatc
      301 attaggcgac atatcctctt cactatgtcg catataaccg ataactctgg cccccgtttc
      361 cataatcaac tcgttatcga ggttttctgt tctggcgcgc agcaaacgtt gctcggtctt
      421 ttgcacactc agtatggcca ttttagccat aaacagatcg gcagcttcct cttctttttg
      481 tcggttatcg ctgaaaatcc ccacattttt ccccagtaat aacggcagaa taataatgcc
      541 gcaaaccagc gaaaagagga tcacacctgt ggcgagaaat accagcaagt aacgcccggg
      601 aaaaggtgtg ccatccggca gcaataacgg gatagaaagc acccccgcaa gcgtgatagc
      661 tccgcgcacc cccgcaaagg tcgccagcag catatgacgc gttttatatc cagcaaacag
      721 catcggatgt ttgccgggtc gtttacggct atacagacgc atcagccaaa gccagcccag
      781 gcgcacaacc attaacgcca ggtagatcca aatgacatca aggagcagcg atccggtgtc
      841 caccagtgga tcgttttctg cacggatcag tgattccgaa ataatgcccg gcagttgtaa
      901 gcctaaaatc agaaacacca tgccgttaaa gacatattca agcatcgacc agctactttt
      961 agctttaatt ctcaggcgaa taggcgcgtg gtggactacg ccaatgcgac tggttgttaa
     1021 ccccgcggcg acagcggcaa gaatgccaga taagccaaaa tgttccgcaa ccagataaac
     1081 aataaacggc aacagaagca tcaatacaat ctgaatggaa ggttcatcgt gagcatatcg
     1141 gctgatcaac agcagccctt taccatataa ccaggtaaca gcgatcccca cggcaatacc
     1201 gcccaacgca acgacaaaaa acgcaatggt tgcgccatga acgctgaatt ccatcgttcc
     1261 catcgtcact gcaacagcaa atttcaacgc caccaggccg gaagcatcat tcatcaacgc
     1321 ttcgccttcg agcacttcct ggatgttttt gctgatacgc cctttcccga caatccccag
     1381 caacgccacg gcatcggtag gcgacaacac ggcggctaat gcgaaagcag cgatgagcgg
     1441 cacattcggt aagagtaaat gaatcacata gccgacacca aaaatagtga ttagtaccaa
     1501 cacaagtgcc aggctgataa tttcccggct gtgcttgatc aattctttag gttgtatttt
     1561 tgaactttca gcaaacaaca gggggggtat aaacaataag agaaaaagtt caggattgaa
     1621 ttccacatgc agaccaaata taggctgggc cagtactgcg ccagcggcaa tctggattaa
     1681 aggaagtggt atctggacag gagacagacg tgtaagcacg ctggttaatg atactacca
//
LOCUS       DAZFRG010000454         1709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFRG010000454 DAZFRG010000000
VERSION     DAZFRG010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.456"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..252
                     /locus_tag="ACHKUC_005037"
     CDS             55..252
                     /locus_tag="ACHKUC_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6621091.1"
                     /translation="MKSLTTETALYILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQLQLLQQNR"
     gene            337..1182
                     /locus_tag="ACHKUC_005038"
     CDS             337..1182
                     /locus_tag="ACHKUC_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6621092.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQHS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            1251..1646
                     /locus_tag="ACHKUC_005039"
     CDS             1251..1646
                     /locus_tag="ACHKUC_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HFO6621093.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKGQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
BASE COUNT          498 a          407 c          407 g          397 t
ORIGIN      
        1 ctgaatgcct gccggattta cccactttcg actggtaacc cggaaattca caacatgaaa
       61 tcattaacca cggaaacagc gctgtatatt ctgattgcgt ggctgcagga caatattgac
      121 tgcgaatcag ggattatctt cgacaacgat gaggataaaa cggattcagc agcactgctg
      181 ccctgcatta aacaggccag ggaagatatc cgtaccctgc gccaactgca gcttctgcaa
      241 cagaaccggt gagtcttact caccagactt cattccactg acgccagcct gaatacggct
      301 ggcgttttcg tttataaacc aaaaggaaaa acgatgatgt cagatatcac aatctcccgc
      361 ccggaactgg tcaacggaca tacggacgtt atctgctcaa ccagtatccg ccacattctg
      421 gctgtacgaa agagtgcgct gctgcaaatc gacacactta tccggcaact ggcagaaatc
      481 tcagtactga cagaaagtat tggaggtaaa accgccctgg actgggcgat gaaacaggat
      541 tttcgctgcg gctgctggct catggagaaa ccggaaaccg caatgaaagc catcacccgc
      601 aatctcgatc gcgaaatctg gcgtgacctg atgcaacatt ccgggatgct gtcattaatg
      661 gatgcgcagg cccatgatac atggtaccgc tcactggagt acgataactt tccggaaatc
      721 agcgaggcga atatcctgag tacgtttgaa caactgcacc agaataagga tgacgtgttt
      781 gagcgaggtg tgatcaacct cttcagaggg ctgagctgga attacaaaac caattgcccc
      841 tgtaaatttg gcaataaaat tatcgtcaac aatctggtga gatgggaccg atggggattg
      901 catcttatca ccgggcaaca ggcagatcga ctcgctgacc tggaaagaat gctgcatctg
      961 ttcagcggca aaccgatccc cgacagccgg gaaaatatca tcattcggct ggatgatcac
     1021 atccgctccg ttcagggcaa agcgtgctat gaagatgaga tgttcagcat cagatacttt
     1081 aagaagggct ccgcacacat cacgttcagg aaaccagaac tggttgacag gctaaatgac
     1141 ataattgcca ggcactatcc aaatgtatta gcagacaggt aatgtggcct tgccccgcta
     1201 ttaaacatgt catatttttg ctatcattgt gaaaattttt cggaggaaac atgactacaa
     1261 aagaccgtat tcagtcccga ctgaaccgtt caaaacgtta tgtgttcacc cgggatgatt
     1321 tcagggatat cgctggctat gatcaggttg gcagagctct tcgtacactg gtaaacgaag
     1381 gtaagttgat gaaagtgggc tatggtgttt acaccaaagc ccgtcgtaac atcatcactg
     1441 ggaaaatcat gcccgcagcg cctgggggct ctgctgcagt aattacagag gccctggagc
     1501 gcctgaatat cagataccgt ctggcaggag caacggaatg ctacaataaa ggtcagtcca
     1561 cacaaattcc tgtatctctg gggataaaaa caccttctcg atttaaaaga atgttaatag
     1621 tagggaaaag taaaatcaat gcctgaatta acatcattat ttcctgacgt tgccgatgca
     1681 ctggatattg aatctgtcgc tatcgtcga
//
LOCUS       DAZFRG010000455         1707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFRG010000455 DAZFRG010000000
VERSION     DAZFRG010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.457"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..838
                     /locus_tag="ACHKUC_005040"
     CDS             167..838
                     /locus_tag="ACHKUC_005040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6621094.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETLSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            848..>1707
                     /locus_tag="ACHKUC_005041"
     CDS             848..>1707
                     /locus_tag="ACHKUC_005041"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6621095.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVG"
BASE COUNT          431 a          385 c          415 g          476 t
ORIGIN      
        1 ctacataaaa gattacacac tcttatcccc gtacctgaca gccccttgtt tttcagctat
       61 cagctatttt taacacttaa aaattagcat ctgtctgcgt cagaggttac gtctctgggg
      121 gcgtcgttgt gcttgtagta taataaataa aatggagtgt cataaagtgg ataaggtttt
      181 agattcagcg atcctttctt cggcaaataa aagaaagggt atccttgcca ttggcgcgca
      241 tcctgacgat atagaattag gctgtggcgc atcgctagct cgccttgcgc aaaaaggaat
      301 ttatattgct accgtggtga tgactaccgg taattctggc gtagatggaa taatcgatcg
      361 ccacgaagaa tcacgcaacg ctctaaagat attagggtgc caccaaacta ttcatcttaa
      421 ttttgctgac acccgcgctc atttacagct caatgatatg atttctgccc tggaaaacat
      481 cataaaaaat caaattccct ctgatgttga aattatacgt gtatatacca tgcatgatgc
      541 tgaccgccat caggatcatc ttgcagtcta tcaagcctca atggttgcct gtcgcgctat
      601 tccgcaaatt ctcggctatg aaacactaag tacctggctt tcatttatgc ctcaggtttt
      661 tgaatccgtg aaagaagaat atttttctct taaactgact gcgttaaaaa aacataaaag
      721 tcagagccag cgagattata tgcgcccgga gcgtctgcgt gcagttgctc aatttcgcgg
      781 gcaacaggtc aatagcgatc tgggtgaagg ttttgttatt cataaaatga ttctttgagc
      841 aaattgcatg aaaacctgga tctttatatg tatggccgta gcaatattgc tatggtttct
      901 gagtacgtta aggcgtaagc ccagccaaaa gaaaggctgt attgacgcca ttatacctgc
      961 ttataacgaa ggcccgtgtc tggcgcagtc actggataat ctgctgcgta acccttattt
     1021 ttgccgggta atttgcgtta acgacggctc cacggacaat accgaagcgg tcatggcgga
     1081 agtcaaacgc aaatggggcg accgctttgt tgccgtcacg caaaaaaata ccggtaaagg
     1141 tggtgcgctg atgaatggcc tcaattacgc cacctgcgat caggtttttt taagtgatgc
     1201 cgacacctat gttccgcccg atcaagacgg aatgggctat atgctggcgg aaattgagcg
     1261 tggcgctgat gccgtaggcg gcattccctc tactgcgttg aaaggcgcgg gtctgttacc
     1321 acacatccgc gcgaccgtaa agttgccgat gattgttatg aagcgcacgc tacagcagct
     1381 tctgggcggc gcaccgttta tcatcagcgg tgcctgcggg atgttccgta ctgatgtatt
     1441 gcgtaagttc ggtttctcgg atcgtactaa agtcgaagac cttgatctca cctggacatt
     1501 ggtggcaaac ggctatcgta ttcggcaggc gaatcgctgc atcgtatacc cacaggaatg
     1561 caacagcccg cgtgaggaat ggcgtcgctg gcggcgttgg attgtgggct acgcggtctg
     1621 tatgcgcctg cataaaagac ttttatttag ccgcttcggt atcttcagta tatttcctat
     1681 gctgttggtt gtgctttatg gcgttgg
//
LOCUS       DAZFRG010000456         1681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFRG010000456 DAZFRG010000000
VERSION     DAZFRG010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.458"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..140
                     /locus_tag="ACHKUC_005042"
     CDS             33..140
                     /locus_tag="ACHKUC_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621096.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            346..1629
                     /gene="gltA"
                     /locus_tag="ACHKUC_005043"
     CDS             346..1629
                     /gene="gltA"
                     /locus_tag="ACHKUC_005043"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO6621097.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          432 a          415 c          401 g          433 t
ORIGIN      
        1 gttgttacaa acattaccag gaaaagcata taatgcgtaa aagttatgaa gtcggtattt
       61 cacctaagat taacttatgt aacagtgtgg aagtattgac caattcattc gggacagtta
      121 ttagtggtag acaagtttaa taattcggat tgctaagtac ttgattcgcc atttattcgt
      181 catcaataga tcctttacct gcaagcgccc agagctctgt acccaggttt tcccctcttt
      241 cacagagcgg cgagccaaat aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt
      301 taagttccgg cagtcttacg caataaggcg ctaaggagac cttaaatggc tgatacaaaa
      361 gcaaaactca ccctcaacgg ggacacagct gttgaactgg atgtgctgaa aggcacgctg
      421 ggtcaagatg ttattgatat ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca
      481 ggcttcactt caaccgcatc ctgcgaatct aaaattactt ttattgatgg tgatgaaggt
      541 attttgctgc accgcggttt cccgatcgat cagctggcga ccgattctaa ctacctggaa
      601 gtttgttaca tcctgctgaa tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa
      661 actacggtga cccgtcatac catgatccac gagcagatta cccgtctgtt ccatgctttc
      721 cgtcgcgact cgcatccaat ggcagtcatg tgtggtatta ccggcgcgct ggcagcgttc
      781 tatcacgact cgctggatgt taacaatcct cgtcatcgtg aaattgccgc gttccgcctg
      841 ctgtcaaaaa tgccgaccat ggccgcgatg tgttacaagt attccattgg tcagccattt
      901 gtttacccgc gcaacgatct ctcctacgcc ggtaacttcc tgaatatgat gttctccacg
      961 ccgtgcgaac cgtatgaagt taatccgatt ctggaacgtg ctatggaccg tattctgatc
     1021 ctgcacgctg accatgaaca gaacgcctcc acctccaccg tgcgtaccgc tggctcttcg
     1081 ggtgcgaacc cgtttgcctg tatcgcagca ggtattgctt cactgtgggg acctgcgcac
     1141 ggtggtgcta acgaagcggc gctgaaaatg ctggaagaaa tcagctccgt taaacacatt
     1201 ccggaatttg ttcgtcgtgc gaaagacaaa aatgattctt tccgcctgat gggcttcggt
     1261 catcgcgtgt acaaaaacta cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa
     1321 gtgctgaaag agctgggcac caaagatgac ctgctggaag tggctatgga gctggaaaac
     1381 atcgcgctga acgacccgta ctttatcgag aaaaaactgt acccgaacgt cgatttctac
     1441 tctggtatca tcctgaaagc gatgggtatt ccgtcttcta tgttcaccgt gatcttcgct
     1501 atggcacgta cggttggctg gatcgcccac tggagcgaaa tgcacagtga cggtatgaag
     1561 attgcccgtc cgcgtcagct gtatacagga tatgaaaaac gcgactttaa aagcgatatc
     1621 aagcgttaat ggttgattgc taagccgttc actttccgga cccgccttta atagagcggg
     1681 t
//
LOCUS       DAZFRG010000457         1677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFRG010000457 DAZFRG010000000
VERSION     DAZFRG010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.459"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..146)
                     /locus_tag="ACHKUC_005044"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="ACHKUC_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060488.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            640..882
                     /locus_tag="ACHKUC_005045"
     CDS             640..882
                     /locus_tag="ACHKUC_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621098.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGINSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            1182..1550
                     /locus_tag="ACHKUC_005046"
     CDS             1182..1550
                     /locus_tag="ACHKUC_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621099.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
BASE COUNT          434 a          461 c          384 g          398 t
ORIGIN      
        1 gtacagagag agtgacgttc cagggcaaca actctttcat gcggttggca ggccaggtgt
       61 tgattacact gatcacgtgg cgtagccacg tttccggctc gattccgtta agttttgcag
      121 ctaccgatca ggctgtacat cactgccgca ctatcgctcg tcatctcaaa gtccggtctc
      181 gtcagcagga aggtatcatt ctctcccgcc atttttccgg gggcccggtc agataagtcc
      241 ctttgtctat cgctgactcc tggctcataa cccggttagc agaatgcagg gtcaccacct
      301 cgccacgacc gaatccggat aagtcaattg caccttctca atcgccattt tgtcacaatt
      361 aagtttgata aatcatgtca cctctgcact gaatctgctc cagtgcctca ttggtctccg
      421 tccacccctt taaactgccg gataagactg ctgcacagcg ccttccccgg aatcaaatca
      481 ctttccctgt ccgccacaat ccgcttcatg ccacctcgta ttttgtttca gagaccggaa
      541 tctgccagaa agtgactacg cgtgcacata tcggttttct gggttacatg catcgtgtta
      601 ttttctgaaa tgcaaaataa taaaaaacaa ggacacataa tgaaggatga aatacacagt
      661 accagcatca acccggatgt tgttgagcag tttctggccc ttatgaatac acattgtaaa
      721 tcctcagaag aggcgctcac catgctgagt acgctttgtt acaacctgat gatgacatgc
      781 ggtatcaatt ccatggacag ccagatgctc aatgggcatg ggatatccat gcaacttcag
      841 gggattgata ttgccgaaag gccaggcaag aatattcact aacctgaaca gggtctgaaa
      901 acgctgattt ttcagcgtct ctgcaggcta aaactgatga tgtaatgaca acaaaatttc
      961 tgcgtttgta ccccaccgat gaaaaagaca aaaactcaca ccgggcttct catcattaaa
     1021 gataaaaccc gacgtgtcag cctctacgaa acgccaacgg catggtgtat cagggggcag
     1081 gagtgctaca gcaaaagtac aggacgccgc tgtggcagcc acgacagtct gagcagactg
     1141 cgcctggaca gcattaagcc tgttgaatga gtggtggcac catgaaagat aacaacacaa
     1201 ccgccacccc agaagcctgg aaaatgcgtt ttctggccaa cgggacatcc ggcacatggt
     1261 tttacactga cgacattgca gtcactgacg cgtggcttcg cgcgcacccg gatgatgtgg
     1321 aggttatccc tctctgccgg atacagcccc gccagcattc ccatgaacaa catattgaag
     1381 aactccgcgc agccctggcc cggatgctcc atatgcatca tatgatgctg gaaaccacac
     1441 attaccggga aaatatcacc gatgcccgtt gtatccgtga aatgcatgaa gcgccattac
     1501 aggcgctcaa tattctgtcc agatacccgc ataaaaagga cgaggtgtga cgcatgtggc
     1561 tactgatttt ctggctttca tcgcccttta atgtctcttc cggtgatgcc cgtcagccca
     1621 cactggcggt gcacagccag gcctttcata ccgaacgggc ctgccgcgat gcattgc
//
LOCUS       DAZFRG010000458         1674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFRG010000458 DAZFRG010000000
VERSION     DAZFRG010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.460"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..1338
                     /locus_tag="ACHKUC_005047"
                     /pseudo
     CDS             131..1338
                     /locus_tag="ACHKUC_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1373..>1674)
                     /locus_tag="ACHKUC_005048"
     CDS             complement(1373..>1674)
                     /locus_tag="ACHKUC_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621100.1"
                     /translation="WRWYCHEKNGYLLSQSDNAYNSQLLCIENAKKQGYSDESVLPLF
                     LHISYIQEKGWKWYQCYGCGYIVKQASVFFPTYQACIIDAEQECIGILVQLSEKT"
BASE COUNT          484 a          354 c          433 g          403 t
ORIGIN      
        1 tccagctgcg gatgcaccaa ggtatgcgaa taaactaact agactggccc cctgaaatac
       61 cagacagtag atgtatctta aaataagagg taggcttgga tatatgtcta acactagttc
      121 taattttgag attgctggcg ttttgttagg aaaagaggtg cgtaaacgta aaacacctca
      181 gaaaaaaaat cgcaattatt caacagacta tggagcctgg catgacggtg tcccacgtgg
      241 cacgtttgca tggcattcag cccagcctgt tgtttaagtg gaaaaaacaa tacctggaag
      301 gaagcctcac tgctgttgct gccggtgaag acgttgttcc tgcttctgaa ctggccgctg
      361 cgataaagca gattaatcag cttcagcgtt tactggacaa gaaaacaatg gaagttgaga
      421 tcctcaaaga agcggtggag tacgcacaat cgcgaaaatg gatagcgcat gtgcccttgt
      481 taccttcgga caaggaatag ctccggtaag ccgtgttctg ggcgtgtcgc gtgcgcagct
      541 aacattacgg atgaaggcat ctgataacaa gccagataaa cggcgtcaga gacgcgatga
      601 ggctgcggat gcagaagtct tatcgcgcat cctggatatc attggcgata tgccagcata
      661 tgggtatcgc cgtgtctggg cgatactcag acggcagtcc cgaaatgaag gcttgccgtt
      721 cgtgaatgca aagcgcgttt accgaataat gagcgaaaac agcctgttgt tgttacatga
      781 caaaccatca cggctgcagc gggaacataa aggccgaata tcggtgaagg aaagcgacca
      841 gcgttggtgt tctgatggct tcgagttcgg ttgtgatgat ggcgaaaaag tccgggttac
      901 gttcgccctg gactgttgtg atcgtgaagc catcgactgg gcagccagta cgggaggtta
      961 cgataaagcg acagtacagg atgtgatgtc aggagcgata gaaaaaggct ttggcgataa
     1021 agtgccggaa gagcccatcc agtggctgac agataacggt tcagcgtaca gagcgcatga
     1081 aacacggcag ttcgccagag agctgaatct ggagccctgc acgacggcag tcagcagtcc
     1141 tcagagtaat ggaatggcag atggttcgtg aaaacgatga aggaagacta catcgcgttc
     1201 atgccgaaac cgaatgtaag aacggcagtg cataaccttg cagtagcgat cgagcattac
     1261 aatgaaaacc atccgcacag tgcgttgggt tatcgctctc cgcgagaata tcgacgtcag
     1321 cgggtaacgt taacttaaga tacatccact gtctggcatt tcaaggggtg gctcaagttt
     1381 tttcagacag ttgaactagt ataccaatac attcttgttc agcatcaatg atacatgctt
     1441 ggtatgtcgg aaagaaaaca gatgcttgtt ttacgatata tccacagcca taacattgat
     1501 accatttcca gcctttttcc tgaatgtagg aaatatgtag aaaaagtggc aagactgatt
     1561 cgtctgagta cccctgtttt ttagcatttt caatgcataa caattgcgaa ttatatgcgt
     1621 tatctgattg agacaaaaga tatccatttt tctcatgaca ataccatctc catt
//
LOCUS       DAZFRG010000459         1673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFRG010000459 DAZFRG010000000
VERSION     DAZFRG010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.461"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..1159
                     /locus_tag="ACHKUC_005049"
     CDS             17..1159
                     /locus_tag="ACHKUC_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO6621101.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          505 a          360 c          361 g          447 t
ORIGIN      
        1 catttgaggt attgttatga caaataaaca cccatcattg ttttctcctt ttatgctgac
       61 agaaaaaata aagctacgta atcgtatcgt tatggctcct atgacaacct ggtcagctaa
      121 tcctgatgga accatctctg agcaagaact cgaattttat aaacgtcgct cacaaaatgt
      181 tggtttagta ataacaggat gtacctatgt cacgccaagc ggcattgggt ttactcatga
      241 atttgccgcg tatgacgacc gtttcattaa cagccttgaa aagttagcag ctgcagccaa
      301 aagtggcggt gctccggcta ttttgcagat tttccacgcc ggtaataaag ccatcccgga
      361 gcttgtgcca aataacgatg ttattagtgc cagcgcatca agcgtaaaat ctggtgactt
      421 tatgaagaga gtggtacaaa gcagggaaat gactgaaaac gaaattcagg aaaccattag
      481 agcattcgga gatgtcacaa aaagggcgat taaagcaggt tttgatggcg ttgaacttca
      541 tggtgcgcac ggattccttc tgcagaattt cttctcccct ctatttaacc agcgtaatga
      601 ccgatgggga ggagatcttg aaggacgtat gcgcttcccg ctggccgtat tacaggaagt
      661 aaaaaatgta gtctatgaat atgcgacaaa gccctttgct atcggttaca gaatatctcc
      721 tgaagaatct gtaacagggg ggctgcggat agaggatact tataaactcc ttgatcgact
      781 catttcatcc ggtatttcgt atatacatac ctcgctggtc agtattaacg atagctaccc
      841 tgttgagtcg ccaaatggcc cccgaactat tgaattgatt ttaaatcata ttgcaggcag
      901 agtgccggta atcgccgcag gtaaaatacg cacaccgtct caggctcaag aagcaatttc
      961 cgctggcctg ccacttgttg cgatcggtaa agggctggtg ataaatccag aatgggtgac
     1021 tttagctgaa tcaggtagaa gtcatgaaat tcaaacagcc cttaatccac aacgcgttcc
     1081 tgaattaacc attccggata aactatggga tcaaattcag gcatcgaaag gaactggctg
     1141 gtttccgtta atggattaaa tattgattac tctcccgaga gtaaaactat caaatcaatg
     1201 agtaaccgat acagcctcgc aaattctttc agataattaa cccgtcactg cggcacgttc
     1261 tctcgcaagg cgtgcctgca gtgtaatcca gtatgtatcc ggcatagttg acggtttaat
     1321 agactcaatt cctctccctg gttacctaca acaatatctt gtataacacc ccaggagata
     1381 cgacgttacg atgagatgcg atatgctcct cagcgccaat caacgccagg actgggccag
     1441 tcttgagatc agactcattc ccgtaactga tttcaggcga catatatcaa tgacgttggg
     1501 gcaataaaag atagccgatg taatacatct attcaaattt gtgatacatg tcaaaaaatt
     1561 ggttgaccaa actcgttatt tatataaggg cacttacgaa gtgcactctt ttttaaagcg
     1621 aggaagtacc aatgaaagag aataaagtac agcaaatcag tcataaactg att
//
LOCUS       DAZFRG010000460         1659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFRG010000460 DAZFRG010000000
VERSION     DAZFRG010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.462"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1659
                     /gene="tssI"
                     /locus_tag="ACHKUC_005050"
     CDS             <1..>1659
                     /gene="tssI"
                     /locus_tag="ACHKUC_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO6621102.1"
                     /translation="GVVAGFGMQENNGWQMRYHLRIEPPLWRCGLRRNFRIFQQQDIR
                     TISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARLWAEEGIFFFERFAAD
                     SPEQKLTLCDDVAGLSQAGELPFNPDTSAGAETECVSMFRYEAHVRPSSVQSQDYTFK
                     VPDWPGMYEQQGENLNGQLEQYEIFDYPGRYKDEQHGKDFTLYQMDSLRSDAEKATGQ
                     SNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGSQPQALHGSQGRGTTLGNQLEVI
                     PADRTWRPRLQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVQFHWDRYNPATEASSC
                     WVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPVVMGRTYHEDNRSPGDLPGTK
                     TQMTIRSKTYKGSGFNELRFEDATSNEQVYIHAQKNMDTEVLNDRTTDVKHDHTETIG
                     NDQKITVGLGQTVNVGSKKEGGHDQKVTVANDQTITVQNDQRLDVTHDRHKDVGNDQI
                     SKIIGKDTEEVVKSQDIKVGEDYSLTVTNSLTIKVGECLLKMNKD"
BASE COUNT          427 a          372 c          563 g          297 t
ORIGIN      
        1 ggcgtggtgg cagggtttgg tatgcaggag aacaacggct ggcagatgcg ttatcacctg
       61 cgtattgagc cgccgttatg gcggtgcggg ctgcggcgga acttccgtat cttccagcag
      121 caggatatcc ggacgatatc ggccacatta ctgaacgaga acggcgtcac ggagtggacg
      181 ccgctgtttt atgaggacca tccggcgcgg gaattctgtg tccagtacgg ggaaagcgac
      241 ctggcgtttc tggcgcggct gtgggcggag gagggtattt tcttctttga gcggtttgcg
      301 gcggacagtc cggagcagaa gctgacgctg tgcgacgatg tggcggggct gtcacaggcg
      361 ggagagcttc cgtttaaccc ggacacatcg gcaggagcgg agacggagtg tgtcagtatg
      421 ttccgttatg aggcgcatgt ccgcccgtcg tcggtgcaga gccaggatta cacgtttaag
      481 gtgccggact ggccggggat gtatgagcag cagggtgaga acctgaacgg acagcttgaa
      541 cagtatgaga tatttgatta tccgggaagg tacaaggatg aacagcacgg caaggatttc
      601 acgctgtacc agatggacag cctgcgcagt gatgcggaaa aggccacagg gcagagtaat
      661 tcaccgaagc tgtggccggg aacgcggttt acgctgacgg gacatccgca gaagatgctg
      721 aaccgtgaat ggcaggtggt acagagcata ctgtcaggaa gccagccgca ggcgctgcac
      781 ggcagccagg ggagaggaac cacgctgggc aatcagttag aggtgatacc ggcggaccgg
      841 acgtggcgtc cacggctgca aagcaaaccg aaggtggacg ggccacagag cgccattgtc
      901 accggtcctg cgggagagga aatcttctgc gacgaacacg gacgtgtgcg agtgcagttt
      961 cactgggaca ggtataaccc ggcaacggag gcgagttcgt gctgggtgag ggtgtcacag
     1021 gcgtgggcgg ggccgggttt cggcaatctg gcgataccgc gggtgggcca ggaggtgatt
     1081 gttgatttcc tgaacggaga cccggaccag ccggtggtca tggggcggac gtaccatgaa
     1141 gataaccgct ccccgggtga cctgccggga acgaagacgc agatgaccat ccggtcaaaa
     1201 acctataaag gaagcggatt taacgagctg cggtttgaag acgccaccag taatgagcag
     1261 gtgtatatcc atgcgcagaa gaacatggat acggaggtgc tcaacgaccg gacaacggat
     1321 gtgaaacatg accatacgga aaccatcggt aacgaccaga agataacggt ggggttgggg
     1381 cagacggtga atgtggggag caaaaaggaa ggtggtcatg accagaaagt gacagtggcg
     1441 aatgaccaga ccattacggt acaaaacgat cagcggctgg atgttaccca cgatcgtcac
     1501 aaggatgtag ggaacgatca aatcagtaag ataataggaa aagataccga agaagtcgtg
     1561 aaatcccagg atattaaagt tggagaagat tacagcctga cagttaccaa cagcctcacc
     1621 attaaagtcg gtgaatgctt actgaaaatg aataaggac
//
LOCUS       DAZFRG010000461         1653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFRG010000461 DAZFRG010000000
VERSION     DAZFRG010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.463"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..76
                     /locus_tag="ACHKUC_005051"
     CDS             <1..76
                     /locus_tag="ACHKUC_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO6621103.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            108..816
                     /gene="wzzB"
                     /locus_tag="ACHKUC_005052"
                     /pseudo
     CDS             108..816
                     /gene="wzzB"
                     /locus_tag="ACHKUC_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
     gene            904..1125
                     /locus_tag="ACHKUC_005053"
                     /pseudo
     CDS             904..1125
                     /locus_tag="ACHKUC_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
BASE COUNT          482 a          363 c          390 g          418 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgatcggctg taccaaaaaa gtcttcgaca
      121 ttcagtcttc atttatcgga cgttacaata ctgctttctc cgcattagtg gatacgctgg
      181 ataacatgga ggaagccgaa aagctgacta tcgagccaac cgttaaaaac cagccattac
      241 cactggctgt gtcttatgtt ggtgaaaaca cctgaaggtg cacagaaaca actggcgaag
      301 tatatccagc aagttgacga ccaggtaaat aaagaactgg aaaaagacct gaaagacgac
      361 atcgcgctgc gtatgaaaaa cttgaatgat tctctgaaga cccaggaagt ggtggcacaa
      421 gagcaaaaaa cgctgcgtat ccgtcagatt caggaagcgt tgcagtatgc gaatcaggcg
      481 caggtctcaa aacctcaaat tcagcaggct caagatgtga cgcaagatac tctgttcttg
      541 ctggggagcg aagcgctgga gtcaatgatt aagcacgaag cgacccgtcc gcttgaattc
      601 tcatcaaact actatcagac ccgtcaaaat ctgttggata ttgaaggctt aaaagttgat
      661 gatcttgata ttcatgccta ccgctatgtt atgaaaccga cgttacctat tcgtcgcgat
      721 agtccaaaga aagaaatcac cttgattctg actgtgctac tgggtggtat agttggtgct
      781 gggattgtgc tggggcgtaa ttacaacgcg aagtaatctt ttcggttttg gaaaggtggg
      841 cacctacgtt accaatactg gcttaatggc tacatacggc ggtcagttta cgcttacaaa
      901 aatgtggcga aactgattaa tcaaattaag agagagcacg taaaattgta ctttatcgaa
      961 aaccagaccg atccccgttt ggtgaaacaa atcgccaatg ccagtggtgc acaagcggga
     1021 ggagagcttt atccggaagc acttaccgac agtagcggcc tcgctgctac ctacacggca
     1081 gcgttcaaac acaatgtcga taccctcgct gctggcatga agtaagagag taggcaaaaa
     1141 agtaagtaga tatcaggttg ccccatatca tcaggtaatc tgtgacactt ctcttgccag
     1201 agcgccatgt tccagtgcct cactcactat ctgcttatca tccgactttc cctcatacct
     1261 gttcgattat aaagataaaa cgatgatgcc cgggttcatt atgcgaaggc atggcatatt
     1321 tgttcccggt atcgtcagac agcatcattc gactctccat ctgctgtctg gccagacaaa
     1381 agatggcctt gtttgccgcg gtggaaatgg agggaggacg agcccacaag catgaaaaac
     1441 ttaacccagg ctgtggaagt gcaacttttg ctccggttgc tgcagtaaaa cacgagtata
     1501 cgtctgccgt acacattcat aagcgattct gatggctcac ttacgtggag aaatggagtg
     1561 ccgtctgttc tttatttatt gtttgtgtta tgttataaca cataaaagag tattgtttgg
     1621 catctgtaac ttattgagaa ggcaaactgc aga
//
LOCUS       DAZFRG010000462         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFRG010000462 DAZFRG010000000
VERSION     DAZFRG010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.464"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..809
                     /locus_tag="ACHKUC_005054"
     CDS             18..809
                     /locus_tag="ACHKUC_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621104.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINGYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRYIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSGETPVKSAVGNAVILLAKDVIYNEYFFDLRKSFFVSVKDLMTSGTKGIPETQKYP
                     GVYCWIPLFSINSGVVITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1191..1397
                     /locus_tag="ACHKUC_005055"
     CDS             1191..1397
                     /locus_tag="ACHKUC_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6621105.1"
                     /translation="MPPPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARITQSRP"
     gene            1483..>1643
                     /locus_tag="ACHKUC_005056"
     CDS             1483..>1643
                     /locus_tag="ACHKUC_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621106.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGT"
BASE COUNT          454 a          394 c          359 g          436 t
ORIGIN      
        1 taacagaaaa cttcatcatg acaacacgta taacgttcag tatgaccagt gatgagacgc
       61 tgcgaattgt ggatgagtac tgtcataccc acaaactttc gcgctctaag gtaatcaacg
      121 cattactcag cgccacagca cctgtcctta atgatattaa cggttattat caacttgcag
      181 gcgaactgca gtcacgctta ttaaatggag tgtatcaacg ggatcttcct cgcaaacgca
      241 atgtcgtcag tgcagagaag tattgtctgg aaatctggga aaacaaactt ttcaccagaa
      301 gaatcctgga gtttgacagt tcaaatggtg ttctgtacgc gctgaaacat aaaagacact
      361 atcgccggga taagatgatt ggaagggttg aaagtcggta tatcaaagac atttgcgaat
      421 accagatgca gctatccggg gagaagacga aatatgcctg ttttatttat attgaaagaa
      481 caatatacaa tcacgataat ccatctggtg aaacacctgt aaaatcggct gttggtaatg
      541 cagtcatcct gctggcaaag gatgtgatat ataacgagta cttttttgat ctgcggaagt
      601 catttttcgt gagcgttaaa gatctgatga cctctggcac aaaaggtatt ccggagacac
      661 agaaatatcc gggcgtttat tgctggatcc cgcttttttc cataaattcc ggcgtggtca
      721 ttaccccggt ctataaaatt gatcccctga aacccgtaac ggtaaaaaaa ccggataaga
      781 tcactgttgt ttgtaattac agagaataat ttccgtcttc tccgttcacc gcaacagtgt
      841 ttaccttgct ttatcttcca ttcagtaaat acaaacctcc gctctggcag gtacagggcg
      901 tggtttatct ccgatactcc ccagcgcagt aataatcatc actgaaaata ctgatcccgt
      961 cacggcaaaa tgccatctca tattccggca acaacaacct cttccgttta taccgacttc
     1021 actgacaaaa cagacaacct gtattgcctg tacacaaccg tttctctcct agcagaaata
     1081 ttcccctact gattttttac gtttctcccc ggttgttatg ccggtgaagg tggtgcgttg
     1141 ttttcatcat caacgccggt tgagattaac aacatccgga ggagattttt atgccccccc
     1201 cagtttcgct gatggatgac cagatggtcg atatggcgtt tatcactcag ctgaccggcc
     1261 tgaccgataa gtggttttac aagctcatca aggatggggc ctttccggct cccatcaaac
     1321 tgggccgcag ctcccgctgg ctgaaaagtg aagtggaagc ctggttgcag gcacgtatta
     1381 cacagtcccg tccgtaattc ctgaccgtta tccgttcaac cgcagcaaag gcatgatccg
     1441 gcccccggag gcattttgct gcgcgctaaa ggagagaata ttatgcaaaa acttgaaaac
     1501 cggtgtgagc tgctgctcat ccagcatcag aaatggatga caagcgtgac gaggcttatc
     1561 gtcgctcatg gaatggggtc tccgcatctt catggttatc accgcctgac cctggcacac
     1621 ttttttctgc cagaaaaagg cac
//
LOCUS       DAZFRG010000463         1638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFRG010000463 DAZFRG010000000
VERSION     DAZFRG010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.465"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..276)
                     /locus_tag="ACHKUC_005057"
     CDS             complement(<1..276)
                     /locus_tag="ACHKUC_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020236720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO6621107.1"
                     /translation="MRDLKDKIYFYTEGEFLEMLEEIVNATSKDKSLKGKKLEKYLDT
                     LVDHFIKITEHPKKGDLIFYPNSQEDGEPENILKIVKEWRRSQGLPLF"
     gene            complement(282..>1638)
                     /locus_tag="ACHKUC_005058"
     CDS             complement(282..>1638)
                     /locus_tag="ACHKUC_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFO6621108.1"
                     /translation="NTEFDWLAFPAGYNSLFIPPKSPPASNLKPEPLPVVNLPLSSPA
                     VNPVYAKSCLKVKGCTDAGTAEEPAENFGQVAIFAQPVVDDCCGYHHPEANVIGEPAE
                     AQTMLLFPSSVLATQIWGKWSLSGVLSATRGSYIGALASALYIPSAGEGSARVPGRDE
                     FWYEEELRQKALAGSTATTRVRFFWGTDIHSKPQVYGVHTGEGTPYENVRLANMLWNE
                     ERQRYEFTPAHDVDGPLITWTPENPEHGNVPGHTGNDRPPLDQPTILVTPIPDGTDTY
                     TTPPFPVPDPKEFNDYILVFPAGSGIKPIYVYLKEDPRKLPGVVTGHGVPLSPGTRWL
                     DMSVSNNGNGAPIPAHIADKLRGREFKTFDEFREALWLEVSKDSVLLAQFIKSNQNNV
                     SQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAIEHGGGVYDIDNFKIVTPRLHDEIHYR
                     R"
BASE COUNT          416 a          411 c          369 g          442 t
ORIGIN      
        1 aaacaagggt aacccctgtg aacgccgcca ttctttgaca attttcagga tgttttcagg
       61 ttcaccatct tcttgggaat taggatagaa aatgagatct ccctttttag gatgttcagt
      121 aattttaatg aaatgatcca ctaaggtatc gagatatttt tctaattttt tcccttttag
      181 ggatttatct ttgctagttg catttactat ctcctcaagc atttcaagaa attcaccttc
      241 tgtgtaaaaa tatattttat cttttaaatc acgcatatac attatctcct gtagtgaatt
      301 tcatcatgta gtcggggcgt aacaatctta aaattatcaa tatcatacac tccaccacca
      361 tgttctatag caactacatg atgtatctgg aatttcttaa ctatctcatt tggtccgtaa
      421 taatacccct cctcagggac atagggggaa tatccttgac taacgttatt ctgattactt
      481 tttataaact gcgccaataa cactgagtct ttactcacct ccagccacag cgcctcgcga
      541 aactcatcaa aggttttaaa ctcccgtccg cgcaatttat cagcaatatg cgccgggatt
      601 ggtgcgccgt tgccgttatt ggataccgac atatccagcc agcgagttcc tggtgaaagc
      661 gggacgccgt gccctgttac aacaccaggc agctttcgcg gatcctcctt caggtaaaca
      721 tagataggct taataccgga tcccgccgga aaaaccagaa tataatcgtt gaattctttc
      781 ggatcaggaa ccgggaatgg cggcgtggta taggtatctg taccgtccgg aatcggcgtc
      841 accagaatgg tgggctgatc cagcggcggc ctgtcgttac cggtatggcc cggaacattc
      901 ccatgttccg gattttccgg cgtccaggta atcagggggc catcgacatc gtgagcgggg
      961 gtaaattcat aacgctgcct ctcctcgttc cacagcatgt tcgccaagcg gacgttttca
     1021 tacggcgtac cttcacccgt atgaacacca tatacctggg gcttgctgtg aatgtcagtt
     1081 ccccagaaaa aacgcacccg ggtagtggcg gtactgcctg caagcgcttt ctggcgcagt
     1141 tcttcctcat accagaactc atcacgtcca ggcacacgag cactgccctc acccgcagag
     1201 ggaatataca gagcagatgc caacgcaccg atgtaagagc cgcgggttgc actgagtacg
     1261 ccactgagcg accattttcc ccatatttga gtcgccaaca cactactcgg gaacagtagc
     1321 atggtttgag cttctgcggg ttccccgata acgttagcct cgggatggtg gtatccacag
     1381 cagtcatcaa ccactggctg agcgaaaatc gctacttgac cgaagttttc agcgggttct
     1441 tctgccgttc cggcatctgt acatcccttc accttcagac aggatttggc atagaccggg
     1501 ttaaccgctg gtgaagagag tggaaggtta acaactggta gcggttcagg cttaagatta
     1561 ctggcaggtg gacttttagg cggaataaat aagctattat agccagcagg aaaggccaac
     1621 cagtcaaatt ctgtattg
//
LOCUS       DAZFRG010000464         1636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFRG010000464 DAZFRG010000000
VERSION     DAZFRG010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.466"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..1625
                     /gene="mobQ"
                     /locus_tag="ACHKUC_005059"
     CDS             159..1625
                     /gene="mobQ"
                     /locus_tag="ACHKUC_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFO6621109.1"
                     /translation="MAIFHLDFKIVKRSEGMTSVAKAAYHARTRITDDRIGETFDFSH
                     RTDLHGHIILAPLSTPAHIIESSSALWNEVERVERQNNGQTARYFDVAIPVELNNDDK
                     KKLVAEYCQKNFVDKGMIADIVFHDLDSKNPHAHVMLTLKTIGPEGFGKKDRSWNDKK
                     IMIQWRESWATMSNSYLEAAGSEERIDHRSLRTQCADALAHAEEAFSAEEKAFWLAKA
                     TETNRPAMQRVHRAKWNDEESQEQRATEQAQRDHQIEEAKKVYTTFSELPLEIVVDVR
                     SFTITHLAEPEEIVLPDYPATVEQQPIVATATTSRRPAAKSYRDPNKVSKVNVSGKKS
                     PVLVAPEPNTSTQLKTPSLQNTRAMNRAPMKSRKQTKPRQNGLFKRFTLLIVGYIREK
                     FIWARRKPDTIDADHDKRIAENYIYDEVLGVNVPRSEFERRAIFNNGDYKLKPDEIKR
                     FPSRPNQEQPEVNRSIDLTPSMPPEHMRQGSAPKLSPP"
BASE COUNT          536 a          375 c          351 g          374 t
ORIGIN      
        1 tcttaaagac aatgcccacc tatacactgc tacgcagtgt gtggggtcta ccgacggttg
       61 ctgccgcagg ctaaaagcaa aaccaaggtc aacgtcaacc ccgaagtttt tctgacgaaa
      121 aactaacagc agcatttata tttgaggtta tatttttaat ggcgattttt catctggatt
      181 ttaaaattgt gaaacgaagc gaaggcatga cttctgttgc gaaagctgcc taccatgccc
      241 gtacacgtat tacggatgat cgcattggag aaacatttga tttcagccac agaactgatc
      301 tacatggtca tattatatta gcccctcttt ccacacctgc tcatattatt gaaagctcct
      361 cagcactctg gaatgaagtt gaaagagtgg aacgccagaa caatggtcaa acagcccgtt
      421 attttgatgt cgctatacca gtagaactca acaacgatga caagaaaaaa ttggttgccg
      481 aatactgcca aaagaacttt gttgataaag gaatgattgc tgatatcgtt ttccatgatt
      541 tagacagtaa gaacccacat gctcacgtca tgttaaccct gaaaaccatt ggtcccgaag
      601 gcttcggtaa aaaagacaga agctggaatg ataagaagat aatgatccaa tggcgtgagt
      661 catgggccac gatgtcaaac agctaccttg aggctgcagg gagtgaagaa cgtatcgatc
      721 accgttctct caggacacaa tgtgctgatg cccttgctca cgcagaagaa gcattcagtg
      781 ccgaagaaaa agcattctgg cttgccaaag caacggaaac aaaccgccca gcaatgcaac
      841 gtgttcatcg tgctaaatgg aatgacgaag agtctcagga acagcgagct actgaacaag
      901 ctcaacgcga tcatcaaatt gaagaggcca agaaggtcta caccacattc agtgagctac
      961 cactggaaat cgttgttgat gtcagaagct ttacgattac acatcttgct gaacccgaag
     1021 agattgttct tccagactac ccggcaacag tagaacagca gccgattgtg gcaacagcaa
     1081 ctacaagcag aagaccagca gcaaaatctt accgtgatcc caataaggtc agtaaagtaa
     1141 atgtttcagg gaagaaatcc cctgttctgg ttgcgcctga accaaataca tccactcaat
     1201 tgaaaacccc atctcttcaa aacaccagag cgatgaaccg tgctcctatg aagagtagga
     1261 aacagaccaa gccccgccag aatggtttat ttaagcgttt cacactactg atagtaggat
     1321 atataagaga gaaatttata tgggccagaa ggaaacccga taccattgat gctgaccatg
     1381 ataagcgtat cgcagagaac tatatctatg atgaagtgct gggtgtcaat gttccacgct
     1441 cggagtttga aagacgcgcc atatttaaca atggtgacta taaattaaaa cctgatgaaa
     1501 tcaagcgttt cccaagccgt cctaatcagg aacagccgga agtcaataga agcatagatc
     1561 tcacaccttc aatgccacca gaacacatga ggcaaggaag cgcgccaaaa ctgagtccac
     1621 cgtaatacat gactcc
//
LOCUS       DAZFRG010000465         1633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFRG010000465 DAZFRG010000000
VERSION     DAZFRG010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.467"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..457)
                     /locus_tag="ACHKUC_005060"
     CDS             complement(<1..457)
                     /locus_tag="ACHKUC_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFO6621110.1"
                     /translation="MEERNNKYELSALYIAVLSSLPFFFCADVAARSYTFEPSMLNVD
                     GNDIDLSIFESGAQLPGTYYVDIMLNGKLVDTKEMEFSRERNKDGEFVLSSCLTQSML
                     NRYGVKVGDYPELFVNSSNGKVCGDLSVIPGAFSYFDFYNQQLNLSIPNV"
     gene            complement(484..1236)
                     /locus_tag="ACHKUC_005061"
     CDS             complement(484..1236)
                     /locus_tag="ACHKUC_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6621111.1"
                     /translation="MFKAIFTVGSLLLPCIVVAKGIGVTASSNDVVDAQLFSVDLNLN
                     RVVYYHGSLGENVAVKNNQAYPMLVQSMVFDEDKKSPAPFITTPPLFRLDGSQTSRIR
                     IVRTGGEFPQDREKLQWLCVKGIPPQSEDKWAEGTITKNSSFTVQFSVNNCIKVFVRP
                     DTIKGHPEDFADKVIWSKKGTTVLEANNPTPFYLNIKELNIGGRSIKSPNYIPPFGKQ
                     SFSIPGSSVGKIQWRIVTDYGGDSKIYQSEGK"
BASE COUNT          549 a          350 c          254 g          480 t
ORIGIN      
        1 ctacattcgg tatacttaaa ttcagttgtt gattgtaaaa gtcaaaatat gagaatgcac
       61 caggaataac agacaaatca ccacatacct taccattaga actattaaca aaaagttctg
      121 ggtagtctcc taccttaacc ccataacgat tcaacatact ctgagtaaga catgaagaaa
      181 gaacaaactc tccgtcttta ttacgctcac gagaaaactc catttccttt gtatctacca
      241 acttaccatt aagcataata tcaacatagt aagtacccgg taattgagcg ccactttcaa
      301 atatagaaag atcaatatca ttcccatcta cattcaacat agatggctcg aatgtgtagc
      361 ttctggccgc aacatctgca caaaaaaaga aaggcaatga tgaaagaaca gcgatataca
      421 gcgcagacag ttcgtattta ttatttcttt cttccatttc aactcactta ccatgcagca
      481 gaatcacttt ccttctgatt ggtagatctt actatctcca ccataatcag ttactattct
      541 ccactgaatc ttaccaactg aagaacctgg aatactaaag ctttgttttc caaaaggagg
      601 tatataatta ggcgatttaa tactccttcc cccaatattt aactccttaa tgttaaggta
      661 gaagggagtt gggttatttg cttccagaac agtagtacct ttttttgacc atatcacttt
      721 atctgcaaag tcttctgggt gacctttaat tgtgtctgga cgaacaaata ccttgataca
      781 attattcaca gaaaattgta cagtaaatga tgaattttta gtgattgtac cttctgccca
      841 tttatcctcg ctctgaggtg gaattccttt aacacataac cactgtaatt tttctctgtc
      901 ttgaggaaac tctccccctg ttcttacaat acgaatccta ctcgtctggc tcccatctaa
      961 tctgaaaaga ggtggagtag taataaatgg cgctggtgac tttttatcct catcaaaaac
     1021 cattgattga acaagcatag gatatgcctg attatttttt accgctacat tttcacctag
     1081 tgaaccatga taataaacca cacgattcaa attcaaatca acactaaaaa gctgcgcatc
     1141 tacaacatca ttagatgaag ccgttacccc aatacctttt gctacaacaa tacagggtaa
     1201 aagaagactg ccaacagtaa aaatagcctt aaacattatt ctccctgcat ccgcaatacg
     1261 aatatttaac ttttctaaca gttctatata tcttcaatac tagagcttca taagatgcaa
     1321 attcaataaa aaaacaaccg tattttattt ttttatctat ttccaacaac aataaaataa
     1381 cacttcaatt attcgataat taattatcaa acaaagaaaa atcatatgat agagacgtaa
     1441 gcgtacagcg agggccgtat tgatgtcacg aacggtgcaa aaaagtggtt cttccgcaac
     1501 ttgggtggaa gaaaatggat gaaactttct ggtgaacagt aagcgtcgtc tcagcaccgt
     1561 ctggcagatc ctgaaattcc tgagagaata gtggacacca aatatggtgg acgcagtcca
     1621 gcgcgaacgt gac
//
LOCUS       DAZFRG010000466         1606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFRG010000466 DAZFRG010000000
VERSION     DAZFRG010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.468"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..193
                     /locus_tag="ACHKUC_005062"
                     /pseudo
     CDS             1..193
                     /locus_tag="ACHKUC_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(180..1604)
                     /locus_tag="ACHKUC_005063"
     CDS             complement(180..1604)
                     /locus_tag="ACHKUC_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6621112.1"
                     /translation="MTTIACGYTEKNATGNVLLLFLLLLLAHRNTLTSITVLLFLFCC
                     ALYAPAGMTYGKINNSFIVALLQTTTDEAAEFTGMIPVYHFLVSAAIQVFMVIFWRTH
                     RRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLREMQRYEQLSQHRADSWKILP
                     GAPLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAPGLFIDGYTSAAASTVPSLSR
                     TLIYDYEQNPDSGNNVVALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKK
                     GSFASRKTDDMLLLQETERALADKSSPKVIFLHMIGSHPNPCDRLHSWPNHYLEQYPR
                     KIACYLASISKLDNFLGQLDGILRRHSRHFVMLYFSDHGLSVSDSANPVHHDGHVQGG
                     YSVPLIITASDITSHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMV
                     FNGERNVPADSLKPQPLILPVRGK"
BASE COUNT          430 a          395 c          389 g          392 t
ORIGIN      
        1 atgctttatt gtcaaacacg ttatggctga catcaaaaaa accggatttc ctctgccagc
       61 gggtaatcac ctccccggtg ttttcggttg gtctggttac tccagtctgg taattagcaa
      121 gataatcgat ataaacagtg gaaaactcat cgtacataat ctggtgatga acattacgct
      181 tattttcccc tgaccggaag aatcagaggc tgcggtttca gactgtctgc cggtacattc
      241 ctctccccgt taaaaaccat aacgggttca ttatcttcgt ctgtcagcgg attgaatggc
      301 ggtatatttt cagtacgaat gccggtcagc cactgaaaaa tacctgcgaa atgacgggca
      361 ctgattttcc tgctgacgga ctgatgagac gtgatgtcac tggcggtaat aatcagggga
      421 acgctgtagc ctccctgcac atgaccatca tgatgaacag gattggcact atcgctgacc
      481 gacagcccat ggtcagaaaa gtaaagcatg acaaaatgac gggaatgccg gcgaaggata
      541 ccatcaagct gaccgagaaa gttatccagt ttactgatgc tggcgaggta acaggcaatc
      601 tttcggggat actgctccag gtaatgattc ggccaggagt gaagccggtc acacgggttc
      661 ggatgagagc caatcatgtg caggaaaatc accttcggag aggatttatc cgccagtgca
      721 cgttctgttt cctgtaacaa caacatgtca tccgttttac gggaagcaaa gctgcctttc
      781 ttgaggaaaa cggtatgctc cgcatcagaa gcaataacag agatgcgtgt gtcatgctct
      841 cccagttttc cctgattgga tatccaccat gtgctgtatc ctgcttttgc tgccagcgcc
      901 accacgttgt tgccggaatc agggttctgc tcatagtcat aaatcagtgt ccggctcagg
      961 gaaggtacgg tactggctgc tgccgatgta tagccgtcaa taaataaacc gggagccgta
     1021 ttcagccacg gtgtggttgg tacgggataa ccatataccg acatataatc cctgcgcaca
     1081 ctctcaccag tgacaatcac aatcgtgtca tacaacggtg ctcccggcag gattttccag
     1141 ctgtcagccc tgtgctgact cagttgttca taacgctgca tttcacgcaa tgtgtcagtt
     1201 gtccccacaa cggttccttt aaccatccgc aacggccagc tgtttacaga gcataatacg
     1261 aacagcagca gtgccagcca gttacggtga ccacggcggt gtgttcgcca gaaaatcacc
     1321 atgaatacct gaatcgcggc actgaccaga aaatgataaa caggaatcat cccggtaaac
     1381 tccgctgcct catcagttgt ggtctgcaac aacgcgacaa taaaactgtt gttgatttta
     1441 ccgtacgtca taccggcagg cgcatacagt gcacaacaga acagaaataa cagcactgta
     1501 atggatgtga gggtatttct gtgtgcaagg agcagaagaa ggaacagcag caacacattc
     1561 ccggtggcat tcttctcagt gtatccgcat gcaattgtgg ttatga
//
LOCUS       DAZFRG010000467         1605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFRG010000467 DAZFRG010000000
VERSION     DAZFRG010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.469"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1603)
                     /locus_tag="ACHKUC_005064"
     CDS             complement(<1..1603)
                     /locus_tag="ACHKUC_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6621113.1"
                     /translation="MNAMATKLTIEQRLELLENELSQNKQELKATQNELGVYKFRLST
                     LQKSITENKYQSASLAEISATSPVADNIKNENGEQNSSAAAHTINGSQQIAVIESKGD
                     KTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNHGSPQTYAAGSLGRFGNEMSGW
                     FDLTLNQRVYNQDGRTANAVVTYDGNVGQQYNDAWFGDSANENIMQFSDIYLTTRGFL
                     PFAREADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIENWALGVGLFDMSLSRDDVD
                     VYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSAPNKTDQQQDNENDDSYFE
                     MKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFSDASNTMAHGRYYYGDHTN
                     GIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAHSDFDSIRTVIRPAWIWNT
                     WNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGESILGSRPEIRFYGTYINI
                     LDNELSNFKFNENSKDEFMAGIQAEV"
BASE COUNT          421 a          381 c          311 g          492 t
ORIGIN      
        1 agacttccgc ctggatgccg gccataaact cgtctttgct gttctcatta aacttaaaat
       61 tcgataattc gttatccaga atattgatat acgtcccgta gaagcgaatt tctggtcgtg
      121 aacctaaaat actttcaccc actttcaatg catgccagag tgtcgtttta taagccgatt
      181 catttagcgt taccccctgc tgagttttgt tctgttgctt aaaccagcct aattcaagcc
      241 ccgtctgatt ccatgtattc cagatccagg ccggtcttat tacggtgcga atactgtcaa
      301 aatcactatg agcgccactt tcataactat aaacatcttc gccatgagaa tagacaagcg
      361 cgttagccat aataatattg tcagtcagat acatctcgcc ctgagagatt aaacgccagg
      421 cgatcccatt ggtatggtca ccatagtaat agcgaccatg cgccatcgta ttactggcat
      481 ctgagaaact ggcaaaactg ctggcatagg aattattggc aacctgtaac gtaaattcat
      541 taaacgtatc gcgttgcaag ttttgccgta aaacagaagt cagcatccag ctatctttca
      601 tttcaaaata actgtcgtca ttttcattat cttgttgttg atccgtttta ttaggtgcgg
      661 aatatttagc cattaatgac aacgtcgcat catcccataa cgggatattg cgataacgaa
      721 catccacaga attggtattc atctgactgg tacgcgtaaa atcacgagag taaacatcga
      781 catcatctcg gcttaaggac atatcaaaca gccctacacc aagcgcccag ttttcaatcc
      841 ccacacccgc ggcaacatcc gtggttaagg ttttccagtc cagcatttgg atctcatatt
      901 gcgggagttt atgtttgccg acccagaagt ctgcctctcg tgcgaagggt aaaaaacctc
      961 gcgttgtcag ataaatatca ctgaactgca tgatattttc attggcactg tcaccaaacc
     1021 aggcatcgtt atactgctga cctacgtttc catcataggt aacgaccgca tttgccgttc
     1081 taccgtcctg attataaaca cgctgattta aggtcaggtc aaaccaacca ctcatctcgt
     1141 taccaaaacg tcccagagaa cccgctgcat aagtttgtgg tgagccgtga ttaccggtgc
     1201 cccagcctga gcggaagtac ccctgatagc tgaatccaat atcatccttt atatatttac
     1261 tgatatcttt cagggtcacg ctttcgatag tggttttatc gcctttactt tcaataacgg
     1321 caatttgctg cgatccattt atagtatgtg ctgcggcaga cgagttctgt tcaccgtttt
     1381 catttttgat gttatcagca acgggagatg tcgctgatat ttcggcaagc gaggccgatt
     1441 gatatttatt ttctgtgatg cttttttgta atgtcgaaag tcggaattta tatactccca
     1501 gttcattctg tgttgctttc agctcttgtt tattttgcga caattcattt tcaagcaatt
     1561 caaggcgctg ctctatggtt aattttgttg ccatcgcgtt caacg
//
LOCUS       DAZFRG010000468         1598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFRG010000468 DAZFRG010000000
VERSION     DAZFRG010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.470"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..581)
                     /locus_tag="ACHKUC_005065"
     CDS             complement(<1..581)
                     /locus_tag="ACHKUC_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6621114.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVA"
     gene            complement(578..1354)
                     /locus_tag="ACHKUC_005066"
     CDS             complement(578..1354)
                     /locus_tag="ACHKUC_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6621115.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGIGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            complement(1401..>1598)
                     /locus_tag="ACHKUC_005067"
     CDS             complement(1401..>1598)
                     /locus_tag="ACHKUC_005067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621116.1"
                     /translation="SEITRRPNPFMPEEWIRRNETEIDLFIPAEIISAEIFLRLLKRS
                     NRLSEFYPVTNKHYLQCIGSC"
BASE COUNT          474 a          370 c          315 g          439 t
ORIGIN      
        1 atcgccacat cacaaaactc tttcaatgaa ttcaaactat gtgaaaccat caaaaagcta
       61 gactctttat gcctttcttt aaacaattga gcgcacttct ctttaaacct ggcatcaccg
      121 acagctgtta cttcatcgac gatataataa tcaaacttaa atgccatgct taaaccaaag
      181 cctaaacgcg atcgcatacc tgaggagtaa gttttgatcg gcatatcaaa gtacttaccg
      241 agttcggcaa attcttcgac aaactcaatt ttctctttca gctcttcttg cttcgcgtat
      301 aaccgcgcaa caaattttac gttttcgcgt ccggttaaac ttccctggaa tccaccggcg
      361 aggccgactg gccatgatat cgttttattg gtgatgatct ttccgctatc ggggcggtca
      421 atgccaccaa tcattctcag taacgttgat ttacccgcac cattacgacc aataaaggca
      481 acgctttttc ctgaagggat ttcgatgttt aaatccttaa acacatagtg tcgccccgtt
      541 ggcgtacgat atgacttcgt caaattctca atcttaatca tgatgtcagc attgcctcct
      601 cacgagttcg gtataacgcc agaccgatga acagggtgac cagagtgaac attgcaaggt
      661 agttcagact cacgccttcg ctgatatagc caggcataac tgcctcgcgg cttaactcta
      721 cgacatgcac taatgggttc cagagtagat atgaccagta ttgcttcgga atcgaatgta
      781 gagggaacat gatgcaggag atgaaataca gcggcttaag cagtatcggc aggacctttt
      841 gcatttcagg gaaggtttta ccaacgacca tgaatattaa gccgatgcca catgaaagaa
      901 tgattaacaa actccaggtg agcactagtt gtaaaaagtt tgttatctcg aaatattcac
      961 ctgccatcca gacgataagc atgagcaata tataaacagc aacgtaaatc agcgtctcaa
     1021 gcagtgcgcg tgcaatgatc gtatcgatgg gttttactgg tcgataatta aacaacccct
     1081 ggttcgcttc aatagcgcct acagaacgat tgctgatact gctaaagata aaaaagggaa
     1141 tcaggccatt aagtaaaaac accgggaatg agatgtctgg catcgtgcgg tgcataatgt
     1201 aaccaaaaat gcccaacagt atcagcaaat gcgcagaggg ttcgagaatc gcccacaaat
     1261 atcccagacg gaacttacca aagcgtgttc gtatttctcg tagaaataat gcctctacgg
     1321 tgactttctg gacttcaaat ccacttcttg ccatttgatg atgtgatcct aatctcttca
     1381 ggtacgctac cgcccttggc ttaacagcta ccaatacact ggaggtaatg cttatttgta
     1441 actggataaa attcactcag acggtttgac ctttttaata gtcgcaaaaa aatttcagca
     1501 gaaattattt ccgcagggat aaatagatct atttccgtct cgtttcttct aatccattct
     1561 tcaggcatga atggattcgg tcttctggta atttcaga
//
LOCUS       DAZFRG010000469         1593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFRG010000469 DAZFRG010000000
VERSION     DAZFRG010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.471"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..336
                     /locus_tag="ACHKUC_005068"
     CDS             <1..336
                     /locus_tag="ACHKUC_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HFO6621117.1"
                     /translation="YKWFDFEVMALKPLISPTWLNILYIMFGVHGGSYFLGIVETITF
                     LALSIGFFRPAAGILGDIIVIVTGLVTLSLLPQLGKVDSFVIKDVLLLGAGSVLLKYD
                     LKNYLNIKN"
     gene            complement(482..692)
                     /locus_tag="ACHKUC_005069"
                     /pseudo
     CDS             complement(482..692)
                     /locus_tag="ACHKUC_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(835..948)
                     /locus_tag="ACHKUC_005070"
                     /pseudo
     CDS             complement(835..948)
                     /locus_tag="ACHKUC_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1045..1593
                     /locus_tag="ACHKUC_005071"
                     /pseudo
     CDS             1045..1593
                     /locus_tag="ACHKUC_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          412 a          382 c          367 g          432 t
ORIGIN      
        1 tataaatggt ttgattttga agtgatggcc ctcaagcccc tgatttctcc cacgtggctg
       61 aatatcctgt acattatgtt tggtgttcat ggggggagct atttcctcgg catagtagaa
      121 actatcactt ttctagcact gagtatcggc ttcttcaggc ctgctgctgg catactggga
      181 gatattatcg ttattgttac aggtttagtc acactaagtc tgttaccaca actgggaaaa
      241 gttgacagtt ttgtcatcaa agatgtgctg ctgttaggtg ctggtagtgt cctgctgaaa
      301 tatgatttga aaaattattt aaatatcaaa aactgatttt tcacttcggt gatgaacgtt
      361 tgcgtatttt catggaaaga gattacctaa tccccctcgt tctccttcat gttaatacgc
      421 aacgttatgg aaaacaaaaa taccctgagt aaaaaggtcc gtaggcaggc gagtactttt
      481 attttccgct agttttactt tcccatcgaa ggccccatac atcactacgc ccacgaaaca
      541 accgacgaaa taacgcaact ttctcatccg tggataatac ggaaacgcac tgcacaggac
      601 tctgcggttt acgtcgccat tcaatcccat aagcttcaag tgatgtcttc cctcagtgct
      661 gctaattcat cactgtcaga tttatgaacc atattcgtta cctgtatccg aaccactgaa
      721 gcaccacagc agaaattcgg gtcccctaca ctgattacag cgccccctct gctgcctatt
      781 aactcaagca cctcccaaca acttacggaa acttaattct aatacagctg taattcagtt
      841 gtgtcgtggt cggccgcggt tacttttttc gggaagtctg ctacgcatca gaataatacc
      901 gtgttctttc tgatcctgtt tagccaggta gctaagggcg taacgtaaac cgttagactg
      961 gccccctgaa tctccagaca accaatatca cttaaataag tgatagtctt aatactagtt
     1021 tttagactag tcattggaga acagatgatt gatgtcttag ggccggagaa acgcagacgg
     1081 cgtactacac aggaaaagat cgctatcgtt cagcagagct ttgaaccggg aatgacggtc
     1141 tcccttgttg cccggcaaca tggtgtggca gccagccagc tatttctctg gcgtaagcaa
     1201 taccaggagg gaagtcttac tgctgtggct gccggagaac aggtcgttcc tgcctctgaa
     1261 cttgctgccg ccatgaagca gattaaagaa ctccagcgtc tgctcggcaa aaaaacgatg
     1321 gaaaatgaac tccttaaaga agccgttgaa tatgggcgag caaaaaagtg gatagcgcac
     1381 gcgcccttat tgcccgggga tggggagtaa gcttcgtcag ccgttgtctc cgggtgtcgc
     1441 gtgcgcagtt gcacgtcatt ctcagacgag ccgatgactg gaaggacggc cgccgcagcc
     1501 gtcacacgga tgatacggat gtgcttcgcc gtatacacca tgttatcgga gagctaccca
     1561 catatggtta tcgtcgggta tgggcgctgc ttc
//
LOCUS       DAZFRG010000470         1590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFRG010000470 DAZFRG010000000
VERSION     DAZFRG010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.472"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..898
                     /locus_tag="ACHKUC_005072"
     CDS             74..898
                     /locus_tag="ACHKUC_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6621118.1"
                     /translation="MSENENLLLRLRQSVDGYSRTQQKLGEFVLSDPAKVVYLTITEL
                     ARESGTSEASVTRLCRTLGCKGYNEFKMALALDLRQVQSVEHGGDEIDNVVNESVQAL
                     QDTAKLLDRTLLEAATLALHQAQSVQIYGVAASAILGEYLHYKLLRLGKPAQLFSDMH
                     RAAMNATTLSKNTFVVAISSSGSTRDLLHVVKLARKQGVRVLALSNTPRSPLASLSDI
                     QLVAAKPEGPLSAGALNAKVGVMLLVELLTTSLIALDHKYSDVSQQTASATLPLLL"
     gene            1132..1590
                     /locus_tag="ACHKUC_005073"
                     /pseudo
     CDS             1132..1590
                     /locus_tag="ACHKUC_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          410 a          396 c          411 g          373 t
ORIGIN      
        1 tttttaaaat gtctggcatc aatcccctcc tttccgccac actatcaaca gataaacgca
       61 ttaaggattt tacatgtcag aaaatgaaaa cctgctgctg agactgcgcc agagcgttga
      121 tggatatagt cgaacgcaac agaaactggg agagtttgta cttagcgatc cggcaaaagt
      181 agtctacctg acgattaccg aactggcgcg tgaaagcggc accagcgagg ctagcgtcac
      241 acgtctgtgc cgaacgctgg ggtgtaaggg ctataacgaa ttcaaaatgg cgcttgcact
      301 cgatctccga caagtacagt ctgtagagca cggcggtgat gagattgaca atgtggtcaa
      361 tgaatccgtg caagcactac aggacacagc aaaattactc gacaggacct tgctggaagc
      421 agccacgctt gcactgcatc aggctcagtc agtgcagatt tatggcgtag cagccagtgc
      481 tattctgggg gagtatttac attacaaact cctgcgtctg gggaaacccg cacaattgtt
      541 cagcgatatg catcgtgctg ccatgaacgc cacaacactt agtaaaaaca cgttcgtggt
      601 ggcgatttcc agttctggat ccacgcgcga tctgctgcat gtcgtaaaac tagcgcgtaa
      661 gcaaggtgtg cgtgtactgg cgctgagtaa tacgccgcgc agtccgttag cttcgctgag
      721 tgatattcaa ctggtcgcgg ccaaaccgga aggccccttg agcgccggcg cgctgaatgc
      781 caaggtggga gttatgctgt tggtagagtt actcaccaca tcgctgattg cgctggatca
      841 taagtacagc gatgtgagcc agcaaactgc cagcgcgacc ctaccgctac tgctttaaag
      901 atataaaaaa ccgcaaaagc actgagggat ccccatgaat cagcgctaat aaaccaacac
      961 catattatgg tcggttctgg cgaagtgatt aagaacggtg atcagcacca ttcgttttaa
     1021 aattagcgag gagtatcata agatattctt cgctaatgtc agacatctgc tttacgggta
     1081 atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg cgtggcttcc
     1141 atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cgacaaaagc
     1201 actgccggac atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc
     1261 acttacaccg cctctcagcc cggtacgcac cagaaaatca ttgatatggc catgaatggc
     1321 gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt tttacgtcac
     1381 ttaaaaaact caggccgcag tcggtaacct cgcgcataca accgggcagt gatgtgattg
     1441 tctgcgctga aatggacgaa cagtggggct acgtcggtgc taaatcacgt cagcgctggc
     1501 tgttttacgc gtatgacagg atacggagga cggttgtggc gcacgtcttc ggtgaacgca
     1561 ctctggccac acaggagcgt cttctgagcc
//
LOCUS       DAZFRG010000471         1575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFRG010000471 DAZFRG010000000
VERSION     DAZFRG010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.474"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /gene="cvi"
                     /locus_tag="ACHKUC_005074"
     CDS             complement(<1..131)
                     /gene="cvi"
                     /locus_tag="ACHKUC_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HFO6621119.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTF"
     gene            513..999
                     /locus_tag="ACHKUC_005075"
                     /pseudo
     CDS             513..999
                     /locus_tag="ACHKUC_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
     gene            complement(1038..1196)
                     /locus_tag="ACHKUC_005076"
                     /pseudo
     CDS             complement(1038..1196)
                     /locus_tag="ACHKUC_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          376 a          352 c          392 g          455 t
ORIGIN      
        1 aaaaatgtat gcatggataa aaagtccttt cctctaaaaa cacaatcata tatagctaat
       61 gcaatatata ttgcggtggc atttattata aatgcaaata acaactctaa ttttgttctt
      121 tttctatcca ttactttcta tcccattacc acacaaacac taacgataat gattatcgtt
      181 catatagtca agagtgaagg gtagggggcc ctcaaccccc tataaggggt ccgcttggaa
      241 aacggatttt cccacgtcaa gagaattgag ttgaacgagt ggttacatcc aagccagcta
      301 actcactgaa cgtaaaggaa gtacgaaatg ttcctaccgt ccgataacca tttggcttac
      361 ttccgtacat tcccttgcgt agatgttcag aattgtacga tttcggacac taaaaccgct
      421 gttgtcccgc cattctggcg attcctaaaa atcccttggc taaaacatag tcttaacgtg
      481 atttattttc cgaattccaa gcggccccct ttatgctggt tatgctggct gcatggggca
      541 ttagctgggg agccagatta gtcatggtgc tgttctggtg tacctgctgt gtgttttcct
      601 ggtataccgc cgttggatag ctccgctacc ggttgttggt cagctgcgca acgctggcat
      661 accgtggctg gctggtgcga tggctgtgct gtatgtcggt gaatttctgc tcggtaaggc
      721 gctggccctg cctgcagaac catttatgac gaaacttttt gccgagaagt ccatactcga
      781 tgtgagcctg acattgctga ccatctttat ccttgccccg ctgaatgagg aaacgctgtt
      841 ccgggggatt atgctgaacg tcttccgttc acggtacgct ggacgatgtg gctgggtgcg
      901 ctgataacgt cgttgttgtt catcgccgcg cacagccagt atcagaacct gctgaccccg
      961 gcagaactgt tcctggtggg ggtgattaca tcagcggcca gttcgctttc gcgctctgac
     1021 gaactcattt gttgctactg tttactgcgc taggtataaa gctgagattc atacaggctg
     1081 agttcacggg ctgcggcggc cacaccgatg cgttcagcga gtttcagggc ttcgttacga
     1141 aattcaggcg tatgttgttt acggggcttc ttgctgattg atactggttt tgtcatgagt
     1201 cacctctggt tgagagttta ctcacttagt cctgtgtcca ctattggtgg gtaagatcag
     1261 gtatcgtttc agtgagcagg ctgcagcaaa ggcaggtaca gctttcagtg acggagacgt
     1321 ttctagaatg taggggttaa ctgggagttc tgagtcgctg agaatttagc agcagggggc
     1381 agtgagacag caaataacgt ctccctgcct gccatataaa gagctgctca gctactataa
     1441 caaaggcaat ataccgccag tgagaaccat tccggaaatt tttcttttat attagggagt
     1501 aacagatatc aatgctggat tatgggcaat atccggaggt tctctgaact attcagatat
     1561 gatgtctgga tgagt
//
LOCUS       DAZFRG010000472         1571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFRG010000472 DAZFRG010000000
VERSION     DAZFRG010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.475"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..1213
                     /locus_tag="ACHKUC_005077"
     CDS             20..1213
                     /locus_tag="ACHKUC_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6621120.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            complement(1326..>1571)
                     /locus_tag="ACHKUC_005078"
     CDS             complement(1326..>1571)
                     /locus_tag="ACHKUC_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO6621121.1"
                     /translation="NTAVRSPESNGIAESFVKTIKRDYISIIPKPDGLTAAKNLAEAF
                     EHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
BASE COUNT          299 a          428 c          438 g          406 t
ORIGIN      
        1 atggagttga aacattataa tgtcaccagg gatcgaagat acgccccaaa aaccactgtc
       61 ctgctggcca cttgcgttca gtgccgggct tctcggtatc ggacagaacg gtctgctggt
      121 tgtgctccct gttctggtca tacagacaaa tctgagtctg tctgtatggg ctgccctgct
      181 gatgcttggc tcaatgctgt ttctgccatc gtccccgtgc tggggaaagc aaatttcccg
      241 tactggcagt aaacctgtgg tgttgtgggc tctgggagga tatggcataa gctttaccct
      301 gctagggctg ggaagcgtgc tgatggctac cagcgccata acaacagcgg tgggactggg
      361 aatattaatc atcgcccgga tcgcctacgg gctgaccgtg tcggcaatgg tgccagcctg
      421 tcaggtctgg gcattgcaga gagctggaga agggaatcgc atggccgctc tggcaaccat
      481 cagctccggc ctgagttgcg gcaggctatt cgggccgctg tgcgcggcag caatgctggc
      541 cattcatcct ctggcgccac tggggctgct gatggcagcc ccagtgctgg cgctgctgat
      601 gctgctgcgg ttgcccggca caccaccaca gcccacaccg gagtgcaaga gcgtcagtct
      661 gaagcgggat tgtctgcctt atctgctttg cgcaatatta ctggctgcgg cggtgagcat
      721 gatgcagctt ggactttcgc ctgcccttac tcgccagttc gccacggata ccaccgccat
      781 tagccaacag gtggcctggc tgttgggtct gtccgcagta gctgcgctta tcgcgcagtt
      841 cggggtactc cgtccgcagc gcctgactcc ggtggccctg ctcctgagtg ccggagtgtt
      901 gatgagtggt ggtctggcta tcatgctctc cgaacagcta tggttgtttt acccgggctg
      961 tgcagtgctg tcatttggag cagctctggc aacacccgct tatcaacttc tactgaatga
     1021 taagctggct gatggcgcag gcgcgggctg gctcgctacc agtcacacac ttggctatgg
     1081 actgtgcgct ttgctggtgc cgctggtatc gaaaacaggt gtcgcaatcg cgctgattat
     1141 ggcagcatta tttgcaacta tattatttac cattgtgtct gtatttatct ggcattaccg
     1201 tactatcaaa taaagatatg atcatcacct tactggagta aatcagagga tatgagagag
     1261 gacctgcgtc tgatgaaaac atgtcaggca gtgttctggc ttgctgcccc cgcctggatt
     1321 tgcccctata tttccagaca tctgttatca cttaacccat tacaggcccg ctgccgcaga
     1381 tattcccgtg gcgagcgata acccaacgca ctatgcggat gccattcgtt ataatgctcg
     1441 aacgcctctg caaggttctt tgctgccgtt aacccgtctg gtttgggtat gatacttatg
     1501 tagtcacgct ttatcgtttt cacgaagctc tctgctatgc cgttactctc cggactccgc
     1561 accgccgtgt t
//
LOCUS       DAZFRG010000473         1566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFRG010000473 DAZFRG010000000
VERSION     DAZFRG010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.476"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(53..1450)
                     /gene="ccp"
                     /locus_tag="ACHKUC_005079"
     CDS             complement(53..1450)
                     /gene="ccp"
                     /locus_tag="ACHKUC_005079"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO6621122.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKLSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          410 a          416 c          323 g          417 t
ORIGIN      
        1 tattatcgta ataaaaatcg cccaaacact cctgttcggg cgatcaaatt aattattgtt
       61 tatcctgcat atacggcgtg taaaccccgt tcagactgtg caggaaagct acgatatcat
      121 ccacatcttc ctgcggcagc tctttgccta cctgatagcg cagcatcagt ttcaccgccc
      181 cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca acgttacgta
      241 aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc atacgaccaa
      301 tatccgccgc cgtaatttca ccaaagttaa agtctttttt cagccccaac ggttcaaagg
      361 aacgtccgcc gagaataata ccaccatgac aagttgcaca tttattatct ttaaataatt
      421 gatagccttt tttctgttgc gccgtcagag cattctcatc tccacgcaac catttatcaa
      481 atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca gtaatatttt
      541 cgccactgaa accttgcgga tagacttcga ggaactgcgc tttaagctgc ggatcttttt
      601 ccagcttagc aataatttcg tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg
      661 gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc tcaacgttaa
      721 atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg
      781 atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg
      841 tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa acccaacgcc actttttgcg
      901 catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc tccggagcag
      961 tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt atttccgccc
     1021 gctcttcatc actcaactta cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag
     1081 tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg ggtttattag
     1141 ccagcagagc cgcacgcacg gcctcgaggt taaaagattt atatccaagc tttatgtcgt
     1201 aatccatcaa ctgtttcgcg ccgggaatat aataataggc aggtaattct gccgaaggcg
     1261 tatggcaata atcgcatcct ttttcgcgga gaaagcctaa aaccttatta ttttcactga
     1321 cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac cacacataac
     1381 cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta atacgtgaga
     1441 ccattttcat tattcttccc tcacggtagt gagttattgt gacccagaaa attttccaga
     1501 atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagggagat atgctttttg
     1561 atagga
//
LOCUS       DAZFRG010000474         1532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFRG010000474 DAZFRG010000000
VERSION     DAZFRG010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.477"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..340)
                     /locus_tag="ACHKUC_005080"
     CDS             complement(29..340)
                     /locus_tag="ACHKUC_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO6621123.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATKLMASS
                     ESACLQAHKIQTALISQDEGCGKIEVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(368..>1532)
                     /locus_tag="ACHKUC_005081"
     CDS             complement(368..>1532)
                     /locus_tag="ACHKUC_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO6621124.1"
                     /translation="AILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMI
                     AFLVARNVAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVI
                     VALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLIS
                     PAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEA
                     NLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDL
                     AKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYA
                     VPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT          474 a          352 c          327 g          379 t
ORIGIN      
        1 tcctccagct atcgcactgg agaaaaaatc aggcatggag ctcttttctt aatgaaataa
       61 tttccctggc taaatcctgg cataatattg cattcatcaa atgatcctgt gcatgtatca
      121 gaattaaatt aacttctatt ttgccacagc cttcatcctg actaattaac gcggtctgaa
      181 tcttatgcgc ctgaagacag gcagattcag agctggccat tagtttagtt gcgccctgcc
      241 agtctttttg cccggcacaa cgtatggctt ccatcgcatc agaacgagcc tgtcccgcgt
      301 tgatcagtaa ctccataaca agttcttcat ctgcaaacat cacaatctcc ttcctgggta
      361 aatggaatta tccgttagtt gtcatgttat tgactttgtt ggcggcgata acaaaaggta
      421 aatagatcat tgtcgccaca cctaaacata aaaaaccaac cagtaatgcc agccagtttc
      481 ccccggttcc gaggaaagca attaaaggtc cgggcgtagt ccagggcacg gcataagcaa
      541 ccggtggaat aatttccatc gaaacaaaga agtaaccaat ggcacagtta acaatgggaa
      601 ccataataaa cggcaccatc atgatggggt taagtacaat aggaaggccg aacattatcg
      661 gttcattgat attgaaaata ccggggataa atgacatttt tgccagatca cggtattccg
      721 cacgcttaga agcgataaaa atagccaaca ataaccccaa cgtcatacct gaaccgccgc
      781 agttggcgaa tgcatcatta atagaagtcc aggtaatcgg atatggcgcg ccccaggtag
      841 tgccgtgctg agcggcccag gagagattct ccagattagc ctcagaaaaa acggtttctc
      901 gaattgccga cgtggtatta ggtccgtgga tccccagcac ccacaggaaa ttacccacag
      961 cccccaggat aattaccgca aagatattcg ttcccatatg tttgagcggc gtctggatta
     1021 atgtgtaaat gagatcattt aatcctgccg gggatatcaa tgtcaggcaa taattaagtg
     1081 cggaaaagaa caccatgaca aaaaatattg gcaataaaac tttaaatgaa cgcgctaccg
     1141 caggaggtac agctgccggc atcgttatgg tgattttggg gtttcgagct aagcgacaaa
     1201 atatttcact ggtgatcaat gcaacgataa cagcaacaaa caacccctgc gcgccgagat
     1261 atttggtcgt caggaatgcc tgcccatcta tcatctggta tggggtataa acaataaaaa
     1321 atgctcctat tgccgttaat ccacataaaa gatcatcttg ctcatagcta atcgccacat
     1381 tcctggcgac caggaaagca atcataattg acatgatatt tacagaacca ttcattaccg
     1441 gagtaaaaat agcttgtgct ttttcaaggt tggggaaaat gctgttcaga tgcagtaatc
     1501 cggcaataaa accgtcgggc gataatatgg ca
//
LOCUS       DAZFRG010000475         1524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFRG010000475 DAZFRG010000000
VERSION     DAZFRG010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.478"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..160
                     /locus_tag="ACHKUC_005082"
     CDS             <1..160
                     /locus_tag="ACHKUC_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO6621125.1"
                     /translation="ASLGRIPELMRDLASMGQPVKLPTRRERAFPRVVKERLWKYPTA
                     PKKSQSVA"
     gene            complement(445..1062)
                     /gene="tdk"
                     /locus_tag="ACHKUC_005083"
     CDS             complement(445..1062)
                     /gene="tdk"
                     /locus_tag="ACHKUC_005083"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO6621126.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
BASE COUNT          439 a          358 c          313 g          414 t
ORIGIN      
        1 cgcttcccta ggacgtatac cggagctgat gcgcgatctt gcaagtatgg gacaaccagt
       61 gaaattaccg acaagaaggg aaagggcctt cccgagagtg gtaaaggaga ggctctggaa
      121 ataccccaca gccccgaaaa agagccagtc agttgcttaa ctgactggca ttaccccttc
      181 tggggccgtt ttttgttttc ccaaaacaac ttttccataa accggcagca ttgtccttca
      241 tcatatttgc gacgatgtat aacgcctaca cacagcgata ttgtacttta caggttacaa
      301 gtcaatgtcg gtgtataagc gctctgtgag gcgtatagcg gcgttaaaaa actgccgaga
      361 agggtgtata gcccgaaaga agtgcgtaaa acgaactgac aggataaaag cgccctgctc
      421 accctgtcag taaagaaatt cttattaatc gtggcgatgc ctttcctgaa tagccgttaa
      481 tgagccgact tctaacgctt ctttatagtg tttacggcat acagaaacgt agcgttcatt
      541 accaccaata accacctgct caccttcgtt ataaggtctg cctgcttgat caagacgcag
      601 caccatgctt gctttacggc cacaaaaaca gatggttttt aattcaacca gtttgtcgga
      661 ccacgccagt aagtattgac tgccaataaa taattcacct cgaaaatcgg tacgtaaacc
      721 ataacaaagt acaggtatat cgagttgatc gacaacctcc gataattcat atacttgttg
      781 tctggttaaa aactggcatt catcaaccag tacgcaatga attgcctgct gttcatgttc
      841 cgcacgaatc tcatcaaata atgatgaatt ttggttaaat aattttgcag gcgatgacaa
      901 acctatacgc gaactgactt tcccggcacc aaaacgatca tcaatttctg ccgtatatac
      961 gacagtgcgc atgccgcgtt cctggtaatt gtatgaagat tgcaacaatg ctgtagactt
     1021 acccgcattc attgcggaat agtagaaata tagctgtgcc atcagccaca ggccctcaat
     1081 gatatgaaat aaagttggct ggagtttatc ataattcgtc agtttttcag ctcaacagta
     1141 tgcacagaag tggtatatgc gttctccctt acgaagcctt gcataatcct tctgagctat
     1201 cattacaact gcctcgcttg ttataagcgg gtaaatgact gccggtaact attcacaatc
     1261 tttaacctgt tgcgcatgta atagccctct gttgacctcc aggagatagt gcaatactaa
     1321 gtccctgctc ttattgcgac tgttctactt ttcatcattc gcttaatagg gaattctcgt
     1381 aaacacggct aatacagaag actgaaaggt cgtcagccta cgattatctc cccataaaat
     1441 gtgacatgaa tcaggaagtt taaacctcac gtgctgcgaa atcatcggtg caaatagggc
     1501 tatatgccgc gtcttttctg gcta
//
LOCUS       DAZFRG010000476         1521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFRG010000476 DAZFRG010000000
VERSION     DAZFRG010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.479"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..668)
                     /locus_tag="ACHKUC_005084"
     CDS             complement(24..668)
                     /locus_tag="ACHKUC_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO6621127.1"
                     /translation="MSRIITTTVYTLNELSATAQEKALDWYRQHHADSNWYENVYEDF
                     RAVCDIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGLWHWQPAAPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPAGQAMT
                     ADAEAAVSEALRDQAFWLYRQLENEYDWLSSDAAVDEALLINEYTFTEGGLRAG"
     gene            complement(687..908)
                     /locus_tag="ACHKUC_005085"
     CDS             complement(687..908)
                     /locus_tag="ACHKUC_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6621128.1"
                     /translation="MKIITRGEAMRIHQQYPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(971..1447)
                     /locus_tag="ACHKUC_005086"
     CDS             complement(971..1447)
                     /locus_tag="ACHKUC_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6621129.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(1463..>1521)
                     /locus_tag="ACHKUC_005087"
     CDS             complement(1463..>1521)
                     /locus_tag="ACHKUC_005087"
                     /inference="COORDINATES: protein motif:HMM:NF015205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6621130.1"
                     /translation="RDYALQHPECSAIMRIID"
BASE COUNT          326 a          421 c          441 g          333 t
ORIGIN      
        1 aagaatggtt taacgatgac aggtcagccg gcgcgcagac cgccttcggt aaaggtgtat
       61 tcgttgatga gcagggcctc atccacagcg gcatctgagc tcagccagtc gtactcgttt
      121 tccagctgac ggtacaacca gaaggcctgg tcgcgcaggg cttcgctgac agcggcttcc
      181 gcatcggctg tcattgcctg accagccgga ctgttccggg taaccgtgat atccatactg
      241 tacggatggc agtaacgtcc ccggtgactg atttcagcct gtaactgcca gaagttacgc
      301 ttctgtaccg cctgcagggc atccgcgata cgatgaagct cacggtcctg cggggcatat
      361 tccctgattt tccggggggc cgctggctgc cagtgccaga gcccttcaaa acaggctccg
      421 tccccctggc tgcagaatcc gctgaaccag atgcacggct cttccataaa cctgccgttg
      481 ctgagccgga aaacgcgctg gcggaggtca atcccgaaaa tgtcacagac cgccctgaag
      541 tcctcataca cgttttcata ccagttgctg tcagcatgat gctgacggta ccagtcgagg
      601 gctttttcct gtgcggtggc ggatagctca ttcagtgtat acacggtggt ggtgataatt
      661 ctgctcatct gaatgcccgt cctgattcag ttcagggtga cgctcatcag ccggacatac
      721 gggccaaaac tgtccttacg gcgttcagca aacacggcca gcacaccggg aatatcctgc
      781 acctcacgac cggtatacgc ttcagtgctg ccgtgccagc ggtacttacc ggtgcagaac
      841 ggaaaaagac gggatgcagg gtactgttgg tgaatacgca tggcttcacc acgggtgata
      901 attttcataa tgggatacct ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg
      961 ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggctg ccaccgacta
     1021 tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga cgttccgtga
     1081 taaggcggtc tgccttgctg ggtgtgactt caccggacgg gtgattgtgc gccagcacca
     1141 cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatgg acttccgtgc
     1201 ggttgatggt gccggtgaag agggtttcac cggcaatcag ttgattctgg ttattcagat
     1261 acagcacccg gaattcttca cgctccagtc ccgccatgtt cagaatcagc cattcccgtg
     1321 ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggttttca
     1381 gggcccgcag aatgagactg cgctcgccgg gcgtcatctc tccgggcaga aaggaaagct
     1441 gctgcattgt ccctgcctcc attcagtcga tgatgcgcat aatggcgctg cattccggat
     1501 gctgcagggc gtaatcccgc a
//
LOCUS       DAZFRG010000477         1514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFRG010000477 DAZFRG010000000
VERSION     DAZFRG010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.480"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..950)
                     /locus_tag="ACHKUC_005088"
     CDS             complement(<1..950)
                     /locus_tag="ACHKUC_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO6621131.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFSHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQVSCWNDVAFIPATNEKQYQVKYQHAKK"
     gene            complement(976..>1514)
                     /gene="rfaY"
                     /locus_tag="ACHKUC_005089"
     CDS             complement(976..>1514)
                     /gene="rfaY"
                     /locus_tag="ACHKUC_005089"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO6621132.1"
                     /translation="KYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAG
                     LTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHAL
                     NMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYS
                     VIYRTKLRKFIKKLKGKA"
BASE COUNT          462 a          320 c          265 g          467 t
ORIGIN      
        1 tttttctttg catgttggta tttcacttga tactgctttt cattcgtggc tggaataaac
       61 gccacatcat tccagcaaga aacctgataa gcctcattaa aatacttctg gcagggataa
      121 ttcgtccagt cgttccaggg cttcgttacg ccaacataat gaatcagctt agtatcatcg
      181 gtaatggtct ttttataatc ttcatcgctt ttatttttta actcatagtc aatgccataa
      241 aagcaatcat aatcgccgct aataaaaata caatgactaa agaacagaat atttaaaata
      301 tcctgatcaa ggcatttata tttcgtgtgc tgcgggtcac ttgccagcat agcaatggct
      361 ttattcataa aatcattttt cgcccatgct tcaagctgca gatatactac accggcgttg
      421 aaataattcc ctggaagtcc ctctatagcc agacgcttcg ctggtttttc ctgcataaaa
      481 ggcacatctt taataacagc cgcgaactta tcgccatcaa aattaatatc aagcaatcca
      541 gctaatgagc ctttacagat gacatcagca tcgatatata acagattttc aatagtttca
      601 ctcagatact ggaacgcaac taaacggaag aatgttgcat aagaccactg tttcaggctg
      661 gggaagatat caataaaggc actggatact ttataaattc ttacttcaac gttagcattt
      721 ttgctggtaa aagcattaac acgttgtaga taatcttcat caatgtagtc agtaaagaga
      781 tgaaacttaa ccgcaaggtc cgggttatgc ataacaactg attgcataga tactgccgca
      841 ccaaataaaa agttgcggtc aataccgtaa gcgatattta acacacgatc ggtgttagca
      901 atttccccag agacagcgac ttcagtaata ctctcagcta ataaatccat gttggttccg
      961 ttttgactgt gtggtttacg ctttgccttt taatttttta ataaatttgc gtagtttggt
     1021 tctgtagatt actgaatagt agccataatc ttttatttcg ttggcaatac ccaggtgacg
     1081 ttccatggcc agacgatcac gggctttccg ctctgccgta cagcttttac cggataggtc
     1141 aattatcctg acaccatctt tactaacgat aaagttaccc cggtgtgggt cgcccgatag
     1201 catatttaag gcatgcaatt tttccattga agccttaatt tctgctttaa catcttctgg
     1261 aataatcggc atatcattga gctctacgcc ctcgacatac tcgataagca tgataaaaac
     1321 actggcatag ttgaaaattt tacgctcagc cagaaaataa aaatcattag gaaaagtaag
     1381 acctgcactt ctgacacgat cggtttcaac gatcaaattc tgatagtagt caccttttac
     1441 aaaagatttt aaaaaacgtt cattcctttt cgttttcggg gcaaagactt taaaaacata
     1501 gcgaccatat ttcg
//
LOCUS       DAZFRG010000478         1511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFRG010000478 DAZFRG010000000
VERSION     DAZFRG010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.481"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..440
                     /locus_tag="ACHKUC_005090"
     CDS             <1..440
                     /locus_tag="ACHKUC_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO6621133.1"
                     /translation="SKKKFSVENEDLSTVRTSLLNSNLVRKLIMEMSDEDFNQVKDNL
                     EKAIEQRNELKDSLAIIKKYMDDAGVTAEDVYREFGKKLQTGRKNDSGESNKYRYEYN
                     GQIMYWSGQGRRPSVIAEAIKAGGSLDDFLISQGTSSSCSDYE"
     gene            709..837
                     /locus_tag="ACHKUC_005091"
                     /pseudo
     CDS             709..837
                     /locus_tag="ACHKUC_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(867..1216)
                     /locus_tag="ACHKUC_005092"
                     /pseudo
     CDS             complement(867..1216)
                     /locus_tag="ACHKUC_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          421 a          297 c          338 g          455 t
ORIGIN      
        1 tgagcaagaa gaagttttct gttgagaatg aagatctctc tactgttcgt acatcacttc
       61 ttaattccaa ccttgtccgg aagttgatca tggaaatgtc tgatgaagac ttcaatcaag
      121 ttaaggataa ccttgaaaag gccattgagc agcgtaatga gttgaaggat tctttggcaa
      181 ttatcaaaaa gtacatggat gacgcaggtg taacggcgga agatgtgtat cgtgaatttg
      241 gcaaaaaatt acagacgggc agaaaaaacg attcaggaga gtctaataag tacaggtatg
      301 agtataacgg tcaaataatg tactggagtg gacaaggtcg tcggccttct gttattgcag
      361 aagcaataaa agccggaggt tctcttgatg attttcttat atctcagggc acatcatcat
      421 cttgttctga ttatgagtga tacatctaat cggttgaatg ttgctgtaat gctcgctttt
      481 gatgcttata tggcagcgtt accgtttatt gttttagtta caaattggtc gtcatggaca
      541 taacctgaaa ttataactat catcgggtct ttcaagttgc ttatgcatat aacgccaggg
      601 aggaaaacga gagttaaaaa attgaactgt tctcatagag actgtaattt aaattgtgta
      661 attgccggtt tttgatatgt tcactccaac aacgtagaca agtagattgt ggacgaaaag
      721 acgcttaaag tccttgcggc tgaactggct aaaggcctca aaaccggggc cgatctcaat
      781 cagttttccc gtatgctgac gaaattaact gtcgaaacgg cgcttaatgc ggaactcggt
      841 aacggctgca gatgttcctg ctctggtgta tgctcagcat cctgttttgg tgcggcgaag
      901 acatggaagc ggcgggagaa ccccagcgta ttaaccgcaa agttaacttt gcaccgttgc
      961 ccggcaacct ccacctcaac ttcgccctag tcgtgctgga gctggtatcc aggctgagtt
     1021 tcgaagcgaa ctgttctttt tgacggacgc attttacgtt tgggctggat gtagtaacgc
     1081 agcatggaac gtccgcccgt ataacccatt gcctgaccgt tcgttcagag caaccaatct
     1141 gagtcgcaat atcgacaatg tacgcgccct gctggtgcat ttgctttatc atgtaaaagt
     1201 cctctctgct cagcatgttg atgtcatttc tggtgtgaga actttaagaa aacaacatgt
     1261 tgggtggagc ggacaatcca aatggtgacc gtcttattat cgctggcgct gacacctgag
     1321 cgatcacctt taatatggca gcaattacac tgaattactt acagattcgt tataacgaac
     1381 ctctgtacat aattatgaaa cagatttgta tttacataaa acatggatat ttttaattta
     1441 ggtttattac tattattata ctcactccct ttttgatata atctctattg ttatttactt
     1501 cctgtcttta c
//
LOCUS       DAZFRG010000479         1507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFRG010000479 DAZFRG010000000
VERSION     DAZFRG010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.482"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..1367)
                     /gene="rclA"
                     /locus_tag="ACHKUC_005093"
     CDS             complement(42..1367)
                     /gene="rclA"
                     /locus_tag="ACHKUC_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO6621134.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGRNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          412 a          367 c          299 g          429 t
ORIGIN      
        1 ctgagacgcc agaatatttg ttctggcgtc tgattttgag tttatttgac taatgaaaat
       61 agatcattga gtgattcgct catcgacgga tgagtaaata tctgatcgcg taatatgcta
      121 taaggcagcc cggcatccat caccattttc actatattga tcatctcgtg ggagtcaaca
      181 cacagcagtg atgcccctaa tatacgttgg gttttattat caacaatcgc ttttaatacc
      241 ccacgagtat cattcatcac tctggcacgc ggaattgcag ctacaggcaa tgtcaccacc
      301 tgaatatcag caccactctc tctggcctgt tcttctgtca taccaaccct ggacagtggc
      361 ggtgtcatga atacggaata aggcacattt ttccgatcat cagtactacg tctgccttct
      421 cccagcaact catcacgcac aatgcggtaa tcatccagtg aaatataagt aaattgcagc
      481 ccgccggtaa catctcccat cgcccaaata ttgtctgcgg tggtatgtaa ttgcttgtca
      541 acgacaattg ccccgcgctc gtttactgcg atacctgcat tttctggatg taacgaagcg
      601 gtagccggtt gacgaccgga agctattaac agtgcatcca ccgccagttg ggcgtgctcg
      661 ctatgcactt gcacttgatt ttcatggtga ctgattcgct ccacatgggc attgaggatt
      721 atatcgacac cctgatcgcg taaaatcgtc gcgatattat cagcaatatc ccgatcttcc
      781 cgaggcaaaa acagcgaagc tgcctctaaa atggttactt tgctgccaaa attagcgaac
      841 atagaggcga actcaacgcc aatatatccg ccgcccaaaa tacctaaatg cccaggcaat
      901 tcttttagat taagtaatcc ggtgctgtca tatacaccag gcgtggtggt aattccagga
      961 attggcggaa ccacggcttg tgcaccggta ttaataaaaa ttttctcgcc atgaatctcc
     1021 agatttctcc caggccgatg aacacgcagg ctatgattat tgataaactc tgcctggccg
     1081 tcgatcacgt cgatattggg catatccgca agattatgaa aattcttatt acgtaaaaaa
     1141 ttaaccactt catttttacg ctgtatggca cggacaaaat ctgtgtgctg ctgtgcgtca
     1201 tgaaccaatg ttttggttgg gatacagccg atattaatac aggtcccgcc atacattgca
     1261 tttgattgtt cgatgagagc cacacgccaa cctgcttttg ccagcgtgac ggctaatgtt
     1321 tttccagcct tgccaaaacc aataataact gcctgatatt tattcatgat gatttcctgt
     1381 cgaaacgcgt tcatttgaca tcatgactat agacgcaaaa gagcaaataa catttcttca
     1441 caacgcttga tattttgtct tatcgtctgg tattttcttt ttcttaatca gaatcacaaa
     1501 acaaaaa
//
LOCUS       DAZFRG010000480         1504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFRG010000480 DAZFRG010000000
VERSION     DAZFRG010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.483"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..1501)
                     /locus_tag="ACHKUC_005094"
     CDS             complement(29..1501)
                     /locus_tag="ACHKUC_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6621135.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
BASE COUNT          519 a          376 c          258 g          351 t
ORIGIN      
        1 tcattttttt attccctgat agcatccgtt attcttctgc aacattcttt tttcttgctc
       61 cataaaaagg ttttccatcg acagtatcat attcaagcgc agcttctgga tggatccatc
      121 tctgtacacg tgatggcata tcaaaaccaa taaggacaag aacaacaaat gttaagataa
      181 aagcaagaga acacattgct gtaccaagac ttgtatgaga cgcaacaact gcaccaagaa
      241 taggcgcaca tgctccacca agtgaaccta cattataaat aaaccccagt cctgccgcac
      301 gctgctccac attaaaataa ccactgatta ttttcggcac cagagcacca atcccctgac
      361 caaaaacctg ctgagtaaag attagaaggc caagtaacca tacatagtcc cggtccacaa
      421 gaaaaacggg gataataaca agctgaccga taagaagtga tataacatat gcttttcggg
      481 ttccaagcca gtcccccata aaaccaccta tacaacttcc agctgcagag ccaaaacctg
      541 ccagcataaa tatgagagca acagtttctg gtgaatactg catatcaacc ttaagccatg
      601 taggaagaaa agcctgcagt ggccatccgt agaggaaaca gaacataacc acaagcatca
      661 acataatgcc tgttggccat cttttcccca taccctgata tataaaactg ataaaaacag
      721 ccgcacagca caatgccata actgtgatta aagcagcgtt tgcagtgacg cctgaaaaac
      781 aaacatatag acatgcaaat gcaatacaag acagaagaat atttatgtat ttattctttt
      841 ctctgtacag aatatccacc attgttctga cgggtttatt ctcttgttgc cttttttgcc
      901 agtcatcaga ctccggaaga ttcttgcgaa gataaaaagc aaataatatt ggaagcatgc
      961 cgacaaagaa aagagaacgc cagccccata gtggtactac aatgctataa acctgtgcag
     1021 ctaaacctcc cccaatagaa aatccagaaa ttaaaaaacc acttgccttg tttctgagat
     1081 gaacaggcca gctttcgata acatatgtta cacttgagcc atattcacct gccataccaa
     1141 gcccaataac aatgcgagca ataaacatca ctgcatatcc aggagccaga ccacaggcaa
     1201 gggttccgag agagaatagc actatactta aaaccattgc catcctgcgc cccatcttgt
     1261 ccgataaagc gccaatagca agccccccaa accatcgaga aataaaagca gcggaaataa
     1321 ggcttgccgc ttctattgtc gtcaagccga attcatgctg aacctctgta agaaccagag
     1381 atatcagaac gaaatcaaaa ccatcaagta aataacctgt ccatgccgca ccgaatatct
     1441 tccactgtct gggtgaaaga tgcttatacc aaacctgctt tttctgaata caggagtcca
     1501 tatt
//
LOCUS       DAZFRG010000481         1490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFRG010000481 DAZFRG010000000
VERSION     DAZFRG010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.484"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1490
                     /locus_tag="ACHKUC_005095"
     CDS             <1..>1490
                     /locus_tag="ACHKUC_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HFO6621136.1"
                     /translation="VNQDGELDVSGGGHGIDITGDSATVDNKGGMTVADADSIGIQID
                     GDKAVVNNDGDSAISNGGTGTQVNGDEATVNNNGNTTVDGKDSTGTEINGDKAIVNND
                     GDSTILDGGTGTRITGDDATANNSGNTTVDGQGSTGTEIAGNNAVVNQDGELDVSGGG
                     HGIDITGDSATVDNKGGMTVADADSIGIQIDGDKAVVNNDGDNAISNGGTGTQVNGDE
                     ATVNNNGNTTVDGKDSTGTEINGDKAIVNNDGDSTILDGGTGTRITGDDATANNSGNT
                     TVDGQGSTGTEIAGNNSVVNQDGELDVSGGGHGIDITGDSATVDNKGGMTVADADSIG
                     IQIDGDKAVVNNDGDNAISNGGTGTQVNGDEATVNNNGNTTVDGKDSTGTEINGDKAI
                     VNNDGDSTILDGGTGTRITGDDATANNSGNTTVDGQGSTGTEIAGNNAVVDQDGELDV
                     SGGGHGIDITGDSATVDNKGGMTVADADSIGIQIDGDKAVVNNDGD"
BASE COUNT          407 a          384 c          484 g          215 t
ORIGIN      
        1 gtgaaccagg acggcgagct ggatgtgagc ggtggcggtc acgggattga tatcaccggc
       61 gacagcgcaa cggtggacaa caaaggcggc atgaccgttg cggatgcgga ctccatcggt
      121 attcagatag acggcgacaa ggcggtcgtg aacaacgacg gtgacagtgc catcagcaac
      181 ggcggcaccg gcacgcaggt taatggcgac gaagccaccg tgaacaataa cggcaacacc
      241 accgttgacg gtaaggattc cacgggcacc gagataaacg gtgacaaagc tatcgtcaac
      301 aacgacggtg acagcacgat ccttgatggc ggcaccggaa cccgcattac gggtgatgat
      361 gcgacggcca acaacagtgg caataccacc gtagacggtc agggttcgac cggaactgaa
      421 atcgcgggta ataacgcggt ggtgaatcag gacggcgagc tggatgtgag cggtggcggt
      481 cacggaattg atatcaccgg cgacagcgca acggtggaca acaaaggcgg catgaccgtt
      541 gcggatgcgg actctatcgg tattcagatc gacggcgaca aggcggttgt caataacgac
      601 ggtgacaacg ccatcagtaa cggcggcacc ggcacgcagg ttaacggtga tgaagccacc
      661 gtaaacaata acggcaacac caccgttgac ggcaaggatt ccaccggcac cgagataaac
      721 ggtgacaaag ctatcgtcaa caacgacggc gacagcacga tccttgatgg cggcaccgga
      781 acccgcatta cgggtgatga tgcgacggcc aacaacagcg gcaataccac cgttgatggt
      841 cagggttcga ccggaactga aatcgcgggt aataactcgg tggtgaatca ggacggcgag
      901 ctggatgtga gcggcggcgg tcacggtatt gatatcaccg gcgacagcgc aacggtggat
      961 aacaaaggcg gtatgaccgt cgcggatgcg gattcgattg gcattcagat cgacggcgac
     1021 aaggcggtcg tgaacaacga cggtgacaat gccatcagca acggcggcac cggcacgcag
     1081 gttaacggcg acgaagccac cgtgaacaat aacggcaaca ccaccgttga cggcaaggat
     1141 tccacgggca ccgagataaa cggtgacaaa gccatcgtca acaacgacgg tgacagcacg
     1201 atccttgatg gcggcaccgg aacccgcatt acgggtgatg atgcgacggc caacaacagc
     1261 ggcaatacca ccgtagacgg tcagggttcg accggaactg aaatcgcggg taacaacgcg
     1321 gtggtggacc aggacggcga actggatgtg agcggcggcg gtcacgggat tgatatcacc
     1381 ggcgacagcg caacggtgga taacaaaggt ggtatgaccg tcgcggatgc ggattcgatt
     1441 ggcattcaga tagacggcga caaggcggtc gtgaacaacg acggtgacaa
//
LOCUS       DAZFRG010000482         1489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFRG010000482 DAZFRG010000000
VERSION     DAZFRG010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.485"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            551..776
                     /gene="ykgL"
                     /locus_tag="ACHKUC_005096"
                     /pseudo
     CDS             551..776
                     /gene="ykgL"
                     /locus_tag="ACHKUC_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(811..951)
                     /gene="ykgO"
                     /locus_tag="ACHKUC_005097"
     CDS             complement(811..951)
                     /gene="ykgO"
                     /locus_tag="ACHKUC_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO6621137.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(951..1214)
                     /gene="ykgM"
                     /locus_tag="ACHKUC_005098"
     CDS             complement(951..1214)
                     /gene="ykgM"
                     /locus_tag="ACHKUC_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6621138.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          442 a          291 c          324 g          432 t
ORIGIN      
        1 gataagaaga aaaagacatt cagtttcata aggttattgg caatataacg ttcaattctt
       61 acttgcattt tatattagcc aggaactttg ccctgaggtg ttaggaatag tcttaatgtg
      121 ttgtgtaatg gactagtaaa aaaacaagtt taaatgataa gaaatagaat ataaaactat
      181 cataagtcat aattaaagat gatttgccaa tcagtaaggt gttttaatgg attaacatgc
      241 cgcacaaaat ggttatgggt gttcatccca ataagtgatg aaaaattaaa caggtgtaag
      301 tacgtcatct gtccggtaag ccccccaaga gccaaattaa gtgaagttaa acgaagactg
      361 ccagttgtca caggaacgat ggcctctttg ccagggcact gcattcagtc atctgtacgc
      421 gttactctaa ctggcactcg aacgtcggcg atggggttac cgatggatct ggccgttcct
      481 gtggcgtaaa cgtttttatg tcagtaccaa cagatttacc acatttatca tcttaacagt
      541 ttgagtgtaa atgcaggctg cgtcgcaaag ggaggaaact gagtggcgtg ttcagtctaa
      601 aatagagttg atgccagcat acagggcgca gctggtcaac aagtcaacat taatataatg
      661 gaatattcgg agagaaacgt caggcagttg accttaaatg aacaggaaga tacatcccca
      721 ggaaaattaa tgctggtgta ataaatacac caacattaat ccggtctgat tattaatcag
      781 acaaatatcg ttaattcgtc gaattttaaa tcaacgtttt ttcttacgac cctgaacggc
      841 cttaaaacgt ggattagatt tacaaatcac atacagccgt ccttttcgct tcacaatctg
      901 acagtctgga tggcgttctt ttgcggtacg cagagagtta aggactttca tcatgccccc
      961 tttttcgtgc taacaaaacg accaaaacgt tgggtgaacc gtgcaacatt tccttctgat
     1021 gccactgttc tcagcttccc tgtatagaac gggtgcgatt tcgaagagac atcaattgtt
     1081 acgtacggat acgttacgcc atccagctca atctcacggt ctgttttgat agtcgagccg
     1141 attttaaagt actcatcaac gctggtgtcg tggaacacca cagtacgata ctcaggatgg
     1201 atattgggtt tcatcatttt tacctgttat gttataacat aaccgtaaag atacatgctc
     1261 atccggatca tttcaacacc caatcactgt gactatttgt gggatgtgga atttccgcaa
     1321 cggggaagtc tggcattgtt agcagccatt ggcgacgctc ccataaacgc ctggatgagt
     1381 gattgatgat ggtttcgctg agagtatgga tttttcgaga atggaggcta tctccacttt
     1441 actggtcgat gatttattgc agcatgattg attacctgac aatgtgtat
//
LOCUS       DAZFRG010000483         1486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFRG010000483 DAZFRG010000000
VERSION     DAZFRG010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.486"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..896
                     /locus_tag="ACHKUC_005099"
     CDS             186..896
                     /locus_tag="ACHKUC_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6621139.1"
                     /translation="MQTTRTPYSVSFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSKFTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPRIKATMQ"
     gene            938..>1486
                     /locus_tag="ACHKUC_005100"
     CDS             938..>1486
                     /locus_tag="ACHKUC_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFO6621140.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDSKEHSAEKHVPDNSACTPLQDRLADASSEFDAGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEG"
BASE COUNT          419 a          350 c          306 g          411 t
ORIGIN      
        1 aaacttgctg ttatttaaaa tatttacttg gtagcaaggt tttcctatca aaagagaacc
       61 aacatttatt acaaacgcat taagtctgga aacccggcga ctcgccaatg tattagcaat
      121 atttaccgat tgtctgatgt aacggtttcc ccgcttatcc tcttaacaca tggagaatgc
      181 atcagatgca aactacgcgt accccctact ctgtttcctt tatggcgaca gtgttacttt
      241 tattgctctt tgcttgccat agcaccattg ccaacgcagc tgtcgcactt ggtgcgacgc
      301 gggtaattta tccggcaaac cagaaacagg ttttattgcc tgtcacgaat aatgatcctg
      361 ccagtgtcta tctcattcag tcatggatag aaaatgcggg tgaccagaaa gatacccagt
      421 ttgtcattac cccaccgctt ttttccatgc aaggtaaaaa agagaatacg cttcggataa
      481 tcaacgccac taatcatcag ttaccaggcg accgtgaaag cctgttctgg gttaatgtta
      541 aagctatccc tgctatggaa aaagaccaga agaatgagaa tacattacag ctggcaatta
      601 ttagccgcat aaaaatgttt tatcgcccca cgcatctggc aatggcacct gaagaagcgc
      661 ctgcaatgct tcggtttcgt cgcagcggta gcaaattcac tcttattaat ccaacgccct
      721 acttcattac cgtaacaaat atgaaagcag gaaatagcaa tctcccaaac accatggtgc
      781 cgccaaaagg tgaagtatcg gtggatatcc cgcatgctgt aaccggggat atcagttttc
      841 agacaatcaa tgattacggt gcattaactc cgcgtattaa ggcaaccatg cagtaagcca
      901 gtgacctaat taataataaa ctcaatcggg attaaaaatg acagcctttc gtgccgcatt
      961 taaggcatat cgtatgcatc aggtgttaat actgcctcgc tttgcacggc ttacctttgc
     1021 ccttggctta gcaactgcgg tttttcccgt tgacgcagag tattatttta atccacggtt
     1081 tttaagcaat gaccttgctg aatctgtcga tttatctgcc tttactaagg gacgcgaggc
     1141 cccacctggt acatatcggg tcgacattta tttgaacgac gaatttatgg ctagtcggga
     1201 tattactttt attgcagatg ataataacgc agatctcatc ccatgcctga gtacagacct
     1261 tcttgtcagt cttggcataa aaaaatcagc attattagac agcaaggaac attcggcaga
     1321 aaaacatgtg ccggacaaca gcgcatgcac accacttcag gatcgtttgg ctgacgcatc
     1381 atctgagttt gatgctggtc agcaacatct gtcactctcc gttccgcaaa tatatgtcgg
     1441 cagaatggct cgcggctatg tttctcctga tctgtgggaa gaagga
//
LOCUS       DAZFRG010000484         1479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFRG010000484 DAZFRG010000000
VERSION     DAZFRG010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.487"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..1457
                     /gene="gndA"
                     /locus_tag="ACHKUC_005101"
     CDS             51..1457
                     /gene="gndA"
                     /locus_tag="ACHKUC_005101"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO6621141.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAQGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          382 a          361 c          374 g          362 t
ORIGIN      
        1 acattcaagc cgcacaaccc cgcggtgacc acccctgaca ggagtaaaca atgtcaaagc
       61 aacagatcgg cgtcgtcggt atggcagtga tgggacgcaa cctcgcgctc aacatcgaaa
      121 gccgtggtta taccgtctct attttcaacc gttcccgtga aaagacggaa gaagttattg
      181 ccgaaaatcc aggcaagaaa ctggttcctt actatacggt gaaagagttc gttgaatctc
      241 ttgaaacgcc tcgtcgcatc ctgttaatgg ttaaagcagg tgcaggcacg gatgctgcta
      301 ttgattccct gaaaccatat ctcgataaag gcgatatcat cattgatggt ggtaatacct
      361 tcttccagga caccattcgt cgtaaccgcg agctttctgc acaaggcttt aacttcatcg
      421 gtacgggtgt ttccggtggt gaagagggcg cgctgaaagg accttctatc atgcctggtg
      481 ggcagaaaga ggcctatgaa ctggttgctc ctatcctgac caaaatcgcc gccgtggctg
      541 aagatggtga accatgcgtt acctatattg gtgccgatgg cgcaggtcac tatgtgaaga
      601 tggttcacaa cggtattgaa tacggtgata tgcaactgat tgctgaagcc tattctctgc
      661 tgaaaggtgg tctgaatctc tctaacgaag aactggcaca aacctttacc gagtggaata
      721 acggtgaact gagcagttac ctgatcgaca tcactaaaga catcttcacc aaaaaagatg
      781 aagacggtaa ctacctggtt gatgtgatcc tggatgaagc agcaaacaaa ggtacgggta
      841 aatggaccag tcagagcgcg ctggatctcg gtgagccact gtcgctgatt actgagtctg
      901 tgtttgcacg ctacatctct tcactaaaag atcagcgcgt ggctgcgtct aaagtactgt
      961 cgggtccaca agcgcagcca gcaggcgaca aagcagagtt cattgaaaaa gttcgccgtg
     1021 cgctttatct gggtaagatt gtttcttacg cgcagggctt ctctcagctg cgtgctgcgt
     1081 ctgaagagta caactgggat ctgaactacg gtgaaatcgc gaagattttc cgtgctggct
     1141 gcatcatccg tgcgcagttc ctgcagaaaa tcaccgatgc ttatgccgaa aatccgcaga
     1201 tcgctaacct gctgctggct ccgtacttca agcaaattgc cgatgactac cagcaggctc
     1261 tgcgtgatgt cgttgcttat gcagtacaga acggtatccc ggttccgacc ttcgccgctg
     1321 cggttgccta ttacgatagc taccgtgccg ctgttctgcc tgcgaacctg atccaggcac
     1381 agcgtgacta tttcggtgca catacttata agcgcattga taaagaaggt gtgttccata
     1441 ctgaatggct ggattgatcc gatttaacca acaataaaa
//
LOCUS       DAZFRG010000485         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFRG010000485 DAZFRG010000000
VERSION     DAZFRG010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.488"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            701..925
                     /locus_tag="ACHKUC_005102"
     CDS             701..925
                     /locus_tag="ACHKUC_005102"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621142.1"
                     /translation="MLTYNPENNQGTRWLLGTELLRAGKHDRALYVLTGHINEFSPCW
                     YELALLHFMNEAYINAATAFRYGFLTSIYC"
     gene            1108..1326
                     /locus_tag="ACHKUC_005103"
     CDS             1108..1326
                     /locus_tag="ACHKUC_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621143.1"
                     /translation="MFKCAEMLMVEEDDKVCELIFKQQDNFILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLSVRTLGANS"
BASE COUNT          439 a          252 c          296 g          487 t
ORIGIN      
        1 attctacagc gtcatcacct tattcagtcg accttcaaaa taaacgttta acagagacaa
       61 tcaccatttg gattgtccgc tgaggaggcg aacgatttcc tagacagctt tatcatgcca
      121 gcccacattg ttggcgctga caatactaag gacaaacgat ggggaagtag ctttttagcg
      181 ttattttcgg gagtaaaaaa cgtggtatct ttccgtatgt cagttgtatc ttgctggtat
      241 tcgtagtcgt gatagggcat acaacgatat ttatcttgac gttagtgaag aataagttat
      301 tttgtgatct atgttaatct gggattacaa tccgggcttg atacatcgca tcacgacttt
      361 tattttcttc agagtagaat atgttactcc ccgttttaca ggctcaggat ttgaattaca
      421 aattatactt ttaagtgaac aataaccagg ggtatttctt atttcctgaa accaggcttg
      481 gctcatttcg ggatgagttt gaaaaaactc ttgacgcata cgatagtgat gaaataaacg
      541 aaacaaatta cgtcaacaaa ctcaggcgtc ttgcctaaca tgctcctgac atcattgata
      601 ttcacgctta tctggcatat gcatttgcgc tctttatgct gcaattctgg cgaatgttca
      661 tttacagcgt catcaggatg ccgttatact cattgataaa atactaacat ataaccctga
      721 gaataatcag gggacacgct ggttactggg gacggaattg ttacgtgctg gcaagcatga
      781 cagggctctg tacgtattga caggacatat caatgaattt tccccctgct ggtacgagct
      841 tgctctttta cattttatga atgaggcata tattaatgct gcaacggcat ttcgatatgg
      901 atttttaaca agcatatatt gttgaaatgc ttttttagaa tcttcatcca ttccctgggg
      961 ctgtatagta tgatttttca ggtatttctg aatactgcca aatattatta caaaatattc
     1021 tctaaattat gaattaaata tccataagca ttattgcctg ggaactggac atataatcac
     1081 tcttctgtgc taaaggaatg ttctgaaatg tttaagtgtg cagagatgtt gatggtggaa
     1141 gaagatgata aagtatgtga acttattttt aaacaacaag ataattttat tttaaaaata
     1201 aatgatagat ttcccagaat aattataaag aaataccgaa cattacaagg taaagtcgta
     1261 tggctatgga tattgccatt tgttattgaa tggttaagtg tgcgcacttt aggcgcaaac
     1321 agttagtgaa gtaaaagtaa acatcatgat tattgtttgt taaggagtga gatgcctatg
     1381 cgtctggtgt ctattatatt cccaacatta ttggcgctat ttttgctggc gtaggcatcg
     1441 ccacactatg aatattgtgt ttgtcgtgat tcaa
//
LOCUS       DAZFRG010000486         1470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFRG010000486 DAZFRG010000000
VERSION     DAZFRG010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.489"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..>1470
                     /locus_tag="ACHKUC_005104"
     CDS             13..>1470
                     /locus_tag="ACHKUC_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO6621144.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGN
                     KDVTQTYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNVHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKI
                     SGNGTSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVNNSGVSTADSSFT
                     LDGDSSPTISADGQSTYPIVLSLKDSNGKALTGLAD"
BASE COUNT          415 a          277 c          349 g          429 t
ORIGIN      
        1 gttgattgaa ctatgaccac tgtgaataaa aagttaaaaa aaactgcatc tggcgcgatt
       61 acatggtcgg taattgttac ccagatatta tctccagttt ctctttcttt gatcccagca
      121 aacagttttg catcatctgg taataaagat gttacgcaaa cttatgccag cgatgagcat
      181 gcaaataaag tggcctcatt tgcagcaagt gcaggtcaga gcctggcgaa taataatgca
      241 agtagttttg ctgtaaatac tttatcaact caggcgacaa aagaggtcgt cgactggttg
      301 caacaatatg gcaatgcgcg catcaagctt aatgtcgatg attctttttc cttgaaggat
      361 tcatcattcg actttttata tccatggctg gatactcagg attatgtgtt attcagtcag
      421 acatcactac atcgtactga tgaccgaaat cagaccaata ttggtttggg gattcgtcat
      481 ttcactcctg ataacgcaat gttgggtgcg aatgtttttt atgattacga tttaagtcgc
      541 agtcactctc gtgcaggttt tggggttgag tactggaggg attatttcag gcttggtgta
      601 aatacctatt ttggtctatc tgactggaag aacagtcggg atattgatga ttatctggaa
      661 agacctgcaa atggctggga tttttctgct gaaggatggc tacctgctta tccgcaatta
      721 ggggcatcta ttcagtttga aaaatattat ggtaaaaatg tcgggttatt tggaagcgat
      781 aaccttcagg aaaaccctta cgcagttact gggggaattt cttatacacc agttcctctg
      841 attaagtttt ctgcacagca taagcaagga cagagcaacg ttcacgatac aaccttcggt
      901 gttgagttta attatcgccc gggcgtttcc ctggctgaac agcttagcag cgataatgtt
      961 gcagttatgc gagaagtcca gaatcggcgt tatgattttg ttgaacgaaa taacaacatt
     1021 gttctggagt acaagaagaa gcatgcactg aaaatcagtt tgccagagtc tgttcagggg
     1081 gagggggaat caatcatccc tgtaacactg acaatcaaca atgctagtgg tggtatcaag
     1141 tctgtacagt ggaatgatag tgcattcact gcggctggcg gcaagatcag tggaaatggc
     1201 acatcatggc agatcacttt accggcttat aaaagcgaag gtgtaaattc gtggaatgtt
     1261 ggagccacag tccaggataa cagaggcaat gtttccaact atgccgttat gaatatcagc
     1321 gttaataata gtggtgtctc gacagcggat tcatctttta cgctggatgg ggattcgtct
     1381 ccgacgatct ctgctgatgg tcaatccact tatccaatag tattaagcct gaaagacagt
     1441 aacggtaagg cattaaccgg actggctgat
//
LOCUS       DAZFRG010000487         1447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFRG010000487 DAZFRG010000000
VERSION     DAZFRG010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.490"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHKUC_005105"
     CDS             complement(<1..260)
                     /locus_tag="ACHKUC_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621145.1"
                     /translation="MVDIVEADKTDIYFIQESVYGKIGLPAFGNTIGPSAQQVVKKVF
                     AVVKERDKTHAKQRLLLEYNGNKLWMNAIDGSEAILPTEFSKR"
     gene            complement(489..1253)
                     /locus_tag="ACHKUC_005106"
                     /pseudo
     CDS             complement(489..1253)
                     /locus_tag="ACHKUC_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          421 a          329 c          241 g          456 t
ORIGIN      
        1 cgcttagaaa attctgttgg taggatagct tcactgccat caatagcatt catccagagt
       61 ttgttaccat tatattccaa caataatctc tgttttgcat gtgtcttatc tcgttcttta
      121 acgacagcga ataccttttt aacaacttgc tgtgcggatg gtcctattgt attaccaaaa
      181 gctggcaacc cgattttgcc ataaacactc tcctgaatga aataaatatc cgttttatcg
      241 gcctcgacaa tatctaccat ttatccctcc aaacaagacc tgacgccagg acaagagcat
      301 agtgtgtagt aaacacttca tgctcttgcc ctgaccattg aaataacaac tttcatttgt
      361 ttactgtacg ctgtctaaat agctgcgtgg aataaagttg cactttttat catcccttgc
      421 agagcaagat tacgaaagcc cgcccccgca aggactgacg cgaggtagtc tctgtccaca
      481 gctgcttttc acatcttaca tcttgcccct gccttgagta tcttatcttt cgttaaaata
      541 ttaatagcga tcttccgtat ccctgaaaat aattatgctg catttcctct tcttattttg
      601 aagtcgtctt tattcattac cacgtccaga tgccagtgca gcttattctc cacgtaccag
      661 tgatttcggt tcgctgtggc gaacttctct gcggttaaat cagcagaaat gctataatat
      721 ctgtccgtca tttctggctc tttcttttgt tctgttatta ttgaacgaaa ggagactgcc
      781 atgcataatt tcttcagctc tttccattca aacgtgaaat caataagttc atcagggaca
      841 tcgcaaataa tatgaagact gattttaatg acataaatcg ctcccttgcg gcgactcttg
      901 tcataagagt gccgaagcgt ttttccatca attgcaatga catctttatc atctgaggaa
      961 tggcagtcac gcatccagtt aataaagcac tcatgaaatt ttgcaggact gatacaggat
     1021 acaactctgg caatgatatc gtgaacagga ataccatttt caaaatcacc atatcacttc
     1081 aaaaaatcga gatgtgtttc cccaaatcct ctatatcttc ccagcattat gcaccagaaa
     1141 taacggcgca aatagttaac agatagaata tctggcaatt tatgttccac tttccaggct
     1201 tgcctgtaat cgggaataat agaaatatgt tccatcaatt ttttaagttc cattttgtcc
     1261 cccttaatta ggcaatgagt atttgatcat gtataagcaa tgaaaaacag tttcaggaaa
     1321 taaggagtat ctcaattttt aaacataaaa tgagaatcgt ccagtacaaa aaagcagtgt
     1381 tacgtaatct ttccctgatt aaatacgcta ccgcccagcc ctgataacat gatctgcact
     1441 gcgctat
//
LOCUS       DAZFRG010000488         1426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFRG010000488 DAZFRG010000000
VERSION     DAZFRG010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.491"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            362..880
                     /locus_tag="ACHKUC_005107"
     CDS             362..880
                     /locus_tag="ACHKUC_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6621146.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPLEA"
     gene            1101..>1426
                     /locus_tag="ACHKUC_005108"
     CDS             1101..>1426
                     /locus_tag="ACHKUC_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO6621147.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGVIPQRYVTGVVAGFGMQENNGWQMRYHLCIEPPLWRC
                     GLRQNFR"
BASE COUNT          352 a          305 c          393 g          376 t
ORIGIN      
        1 cataagaaat tcctttaagg aatatcttaa ttgcgtgccg gaacgattgt gaagtaagtg
       61 ttgtaatgat tgatttttga agaaattgta ggtttttatg ggggtaagga tacgttatgg
      121 ataattctac tgttgatgtt gatatatttt tagcttatgt gatcgtgttc tgaaattgac
      181 taaaaatata tttaaacacc ataaaagagt ttttcggtag gtgctaatta cgtgtgcctg
      241 taactcgaat aaagaaaagg gtgtggttcc tgatgtgagc cgcatcacac tctaacctgt
      301 cggggattaa cgacgggttg ttcgtaaaac agcagttgat aatttcacaa ggagttcata
      361 aatgccaacc ccatgttaca tttcaattac aggtcagacc caggggaaca tcactgccgg
      421 tgctttcacc gcagattctg tcggcaatat ctacgtgcag ggacacgaag atgaaatgct
      481 ggtgcaggag tttctgcata acgttactgt tccaaccgat ccgcagtctg gtcagccttc
      541 cgggcagcgt gctcacaaac cgttcatttt caccgtggcg ctgaacaaag ccgttccgct
      601 gctgtacaac gcgctggcct ccggtgaaat gctgccgaaa gtggagctgc actggtggcg
      661 tacctctgtt gaaggcaagc aggagcacta cttcaccaca cgtcttactg acgcgacgat
      721 tgtggacatg aacctgcata tgccgcactg ccaggaccct gcacagcgtg agtttacgca
      781 attgctcgca gtatcactgg cctaccgtaa ggttgagtgg gagcacatca aatccggtac
      841 ttctggtgcc gatgactggc gtgcaccgct ggaagcataa atctgagtca acaacatcct
      901 gcctccgtgc aggatgttgt ttttgtacgt gtaaaaaatg agtgtcagta aggagttacg
      961 ctttcatgag ggagatggtg gatgccggaa acccgggcgg tagcgtacct gaaaatgtaa
     1021 agatgagtgt aacgctgcac tggtaccctt tgctgcatgg tgggaatcat ttggtccgtg
     1081 aatgaatcag ggaggtcatt atgtccttaa aaggtcttcg ctttacgctg gaggttgacg
     1141 gccaggagcc ggacaccttt gcagtggtga atttcaggtt aatccagaac cagtcttatc
     1201 cgtttgtgat gagtgtggat gtcgccagcg attcttttat gcagacggcg gagatgctgc
     1261 tggagaagaa ggcaacgcta accatctggc agggcgtcat cccgcagcgt tatgtcacag
     1321 gcgtggtggc gggctttggc atgcaggaga acaatggctg gcagatgcgt taccacctgt
     1381 gtattgagcc gccgttatgg cggtgcgggc tgcggcagaa cttccg
//
LOCUS       DAZFRG010000489         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFRG010000489 DAZFRG010000000
VERSION     DAZFRG010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.492"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..1377
                     /gene="bluF"
                     /locus_tag="ACHKUC_005109"
     CDS             166..1377
                     /gene="bluF"
                     /locus_tag="ACHKUC_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO6621148.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          401 a          299 c          300 g          419 t
ORIGIN      
        1 catttaaatg acaacctata caagtttttt cgccaaaccg tcttcaacct tttccacttt
       61 ttatcaatgt aaagaaacgg ttatgttttg gtattaaaag aaagacacaa gaagatatgt
      121 ctgttaccgt cttactctcg cctcgcccat taccctggat tgttaatgct taccaccctt
      181 atttatcgta gccatatacg tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg
      241 atagcaaatc gcaggaacat gcggtctgac gtaacaggga tcttactgtt taatggttct
      301 cattttttcc agcttctgga aggtccggaa gaacaggttc aaatgatata tcgggccata
      361 tgccaggatc cacggcacta taatattgtt gaactgttgt gcgattacgc gcctgctcgc
      421 cgttttggca aagcaggaat ggaattattt gatttacgcc tgcacgagcg agatgacgtt
      481 ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga tgacagagcg
      541 ctacaatttt ttcgtacttt tgtccttgca actgaacaat caacctattt cgagattcct
      601 gctgaagact cctggctttt tatcgctgac ggatctgata aagaacttga ttcttgtacc
      661 ctttcatcaa atataaacga ccactttgcc tttcatccta ttgtcgaccc tttatcgcgg
      721 cggataatcg cttttgaagc cattgtgcaa aaaaatgaag acagcccgtc agctatagcg
      781 gttgggcagc gtaaagacga ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      841 tttgcgatgg cacacgcact taagctcggt gataaaatga tttcaatcaa tctattacct
      901 atgaccctgg ttaacgagcc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      961 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagtcat atctcggttt
     1021 gatgagtttg ccgaagcggt taaatcgcta aaggctgccg gtatcagtgt agcaattgat
     1081 cattttggcg caggttttgc tggtttgtta ctcctgtcac gcttccagcc tgacagaatt
     1141 aaaatcagtc aggaattgat taccaatgtt cataaaagcg gaccacggca ggcaattatt
     1201 caggcgatca tgaaatgctg tacatcactt gaaattcaag tctctgctat gggtgtggca
     1261 acaccagaag agtggatgtg gcttgaatct gcaggaattg agatgtttca gggagatctg
     1321 ttttccaaag ctaaattgaa tggtatccct tcagttgcgt ggccggagaa aaaataattt
     1381 tcagcacatt atttcgcatg atttctggaa atcatcgcg
//
LOCUS       DAZFRG010000490         1382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFRG010000490 DAZFRG010000000
VERSION     DAZFRG010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.493"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..925
                     /locus_tag="ACHKUC_005110"
     CDS             <1..925
                     /locus_tag="ACHKUC_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO6621149.1"
                     /translation="SNTTLAKTTLKLSHNQDKSHAVHKALSYLADKTKEYSNGELVIR
                     IYPNATLGNERESLELMNSGALQMVKVNAASLESFAPDYSLFSLPFLFRDRDHYYRVL
                     QSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITKPEDLAGMKIRVQQSPSAIAM
                     MKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADMRHAEVAKVYSRDEHTMVPDV
                     LIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAAEKEAYDKMKGMNATVVDVDK
                     SAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            967..>1382
                     /locus_tag="ACHKUC_005111"
     CDS             967..>1382
                     /locus_tag="ACHKUC_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6621150.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSG"
BASE COUNT          419 a          272 c          301 g          390 t
ORIGIN      
        1 atctaatacc acactggcaa aaacaacatt aaaactgagt cataatcagg ataaaagcca
       61 tgctgtccat aaagcattaa gctatttagc ggataaaacc aaagagtatt ctaatggtga
      121 attagttatt cgcatttacc caaatgcgac attaggtaat gagcgtgaat cactggaatt
      181 aatgaactcc ggtgcattac agatggttaa ggttaatgcc gcatcactag aatcatttgc
      241 tccggattac agccttttca gtctgccgtt cttattccgc gaccgtgatc actattatcg
      301 tgttctgcaa agtgatttag gtaaaaaaat acttaattca tcagaaagca aaggttttgt
      361 tggtattacg tactatgacg gcggtgcgag aagtttttat tccaataaac cgattacaaa
      421 acctgaagat ttagcgggaa tgaaaatcag ggttcaacaa agccccagcg ccattgcaat
      481 gatgaaagca ctcggtggtg tcgctacccc gatggcgcaa ggcgaactct acaccgcact
      541 tcagcaagga gtggttgatg gcggagagaa caataccgtt gtctatgccg atatgcgtca
      601 cgccgaagtc gcaaaagtct attcacgtga tgaacacacc atggtacctg atgtgttaat
      661 tatcagcacc gacgtgttga ataaacttgg tgataaagag cgcacggcat tattaaaagc
      721 agccgatgag tcaatgatgc agatgaagga cgtcatttgg cctgcggctg aaaaagaagc
      781 ctacgacaaa atgaaaggga tgaacgcaac agttgttgat gttgataaat ccgctttcaa
      841 agaacgcgtc aaaccgttat acgatgaatt caaggcgaaa gatgcacaat cagccaaaaa
      901 ccttgagcta gtagaaagta tgtaacccta atgggcaggt aattcctgcc ttttcatgga
      961 agacttatgc gaacactcac tcatattctt aataagatcc ttgccggatg ttgctgcatt
     1021 attctcgcca ttatggtgtt ctgcgtaacc tggcaagtta ttgcacgttt tatttttaac
     1081 gctcccagca ctgtgcttga tgagttcacc caaattttat ttatgtggat gattttactt
     1141 ggcggtgtat atactgcggg gttaaaaaaa catctggcca ttgatttgtt ggctcaaaaa
     1201 cttcctgctg catctgttct gacgcttgac agttttatcc aaattatcat tacggtcttt
     1261 gcggttattt tcatgatcta tggcgggaat attgttgttg aaaaagcaca acatgttgga
     1321 caaatctctc cagtattaaa atggccaatg gataaagtat attgggttat gcctgtcagt
     1381 gg
//
LOCUS       DAZFRG010000491         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFRG010000491 DAZFRG010000000
VERSION     DAZFRG010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.494"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..348)
                     /locus_tag="ACHKUC_005112"
     CDS             complement(<1..348)
                     /locus_tag="ACHKUC_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO6621151.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHKUC_005113"
     CDS             complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHKUC_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO6621152.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          353 a          317 c          303 g          392 t
ORIGIN      
        1 ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgccagcgcc
       61 tgccagtgtt gaacagtatt cccgaaatgt agattttccg cgaatatgaa gcaatgcggc
      121 ctcttttatt ttagctgggt tcttggtcgt actaactttt aacaggttcc tggttcctct
      181 taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata
      241 aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc
      301 ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac
      361 ctcttgttag gtgtgagact gcgtagtgtg cacgatcagt tttaccactt caatctggtc
      421 tgtcctttgg ctgtgatatg tacaaagtgt gaaaggggga ttacctgaat tctcccggtg
      481 ggcattttgc aacggaccag ctccgctaca aacgctgttg tgggttcaga ttataacatt
      541 ctgtctaagg ggcgggataa aggtgaattt agggggcatg aaagatgact ttataacctt
      601 gctcacccca gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga
      661 caatcaatgt ttgcgttggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa
      721 cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct
      781 cttcaacatt ccacgccatc agaatggcct ctttaccggg cttacggcgc atttcgaaaa
      841 gtgaggccgc tgcgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg
      901 tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat
      961 ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca
     1021 tacgaagaat tgacatcatc tcttcttgca agcggctcca gtcatcttgc gtatcctggc
     1081 tggtagtttc cagtaatgct ttaactttgc ctacaggaac gccattactt atccaacgct
     1141 taatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc
     1201 gctgtggttt taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc
     1261 cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcata
     1321 aatctccgtc gcctctacac gacccaataa tactttgtac aatat
//
LOCUS       DAZFRG010000492         1337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFRG010000492 DAZFRG010000000
VERSION     DAZFRG010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.495"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..574
                     /locus_tag="ACHKUC_005114"
     CDS             20..574
                     /locus_tag="ACHKUC_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621153.1"
                     /translation="MRDAEFRAMLQASRQRNRYNSYSYTDTPTSYELPKLTQKERKGI
                     DEVIRAITPRERYMPTRKSTKNSIKNYLNNFDSYEQLPSKLDDIFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASSVTNRLQRQATRLSYELPTDAYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYILEEF"
     gene            576..857
                     /locus_tag="ACHKUC_005115"
     CDS             576..857
                     /locus_tag="ACHKUC_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621154.1"
                     /translation="MGNKHTLTRVTKTAFEELNRIKNITGLPHSAVMKLAINRDINEQ
                     DLLNSRLEAARHHIRIKQDDLDSLKYLNAFGLDLARLLSIKIHKLAMEV"
     gene            857..1243
                     /locus_tag="ACHKUC_005116"
     CDS             857..1243
                     /locus_tag="ACHKUC_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621155.1"
                     /translation="MYDFYKHRDHLVRFDGKVWKDVTQTNKPKFTHQQLASRFDIWTE
                     AQKDNGDFEFIRGFDYSYEDDLKDAIKLIENFNSIKNGDKHKANGNRYDKDLIIDAIT
                     KGQSQSYISEKYGLSRSQYYKIKASV"
BASE COUNT          478 a          263 c          258 g          338 t
ORIGIN      
        1 tataaggact ggcttatgaa tgagagacgc agaatttaga gcgatgcttc aggcatcgag
       61 acaacgtaac agatacaact cctacagcta cacagacacc cccaccagct acgaattacc
      121 caagctcacc caaaaagaac gcaaaggcat tgacgaagta attcgggcaa ttacaccgag
      181 ggaacgttat atgccaacca gaaagagcac gaagaacagc atcaaaaatt atttgaataa
      241 cttcgacagc tatgagcaac taccaagtaa gcttgatgat attttcattg gattctgtag
      301 aagtgaaggc cacccgaaat acaacaaaaa actattttat cttctaaaga atcttgacga
      361 aattaacgca tccagcgtga caaatcgtct tcagcgtcaa gcaaccagac ttagctacga
      421 attaccaacc gatgcctatt gtgcattgct tgctgtaatg tgtgcaaagc taattggtat
      481 tgttgaacat catattgccg ttggtaatat tgagccaatg gaaaacgagc aaccagactt
      541 tgagtttgac ccgtatattt tggaggaatt ttaaaatggg aaataaacac acattaacca
      601 gagttacaaa gacagccttt gaagagctta accgaatcaa aaacattaca ggtctgccac
      661 actcagcagt gatgaagctt gcaattaacc gtgatattaa cgaacaagat ttgcttaata
      721 gtcgcttaga ggcagcaaga caccatatca ggattaagca agatgacctt gatagtctga
      781 aatatttaaa tgcttttgga ctggatttag caaggttact aagcatcaaa attcacaaat
      841 tagcgatgga ggtttaatgt atgactttta taaacatcgt gaccatttgg taagatttga
      901 cggtaaagtc tggaaagacg taacacaaac caataaaccc aagtttaccc accagcaatt
      961 agcctcaaga tttgatatct ggactgaagc acaaaaagat aatggtgatt tcgagtttat
     1021 cagaggtttt gattattctt acgaagacga ccttaaagac gccattaaat taattgagaa
     1081 cttcaactct attaaaaatg gtgacaagca caaagccaac ggtaacagat acgataaaga
     1141 tttaatcatt gatgcaatca ccaaaggaca atcacaaagt tatatttctg agaaatacgg
     1201 cttatcacgt agtcaatact acaagattaa agcgtctgtg taagtctgtg agaatgagtg
     1261 agactcaaag atgagctacc ctattactaa ggtgtctcac tccctctgta acccgcatgg
     1321 ttactggcct caggatg
//
LOCUS       DAZFRG010000493         1328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFRG010000493 DAZFRG010000000
VERSION     DAZFRG010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.496"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(110..901)
                     /locus_tag="ACHKUC_005117"
                     /pseudo
     CDS             complement(110..901)
                     /locus_tag="ACHKUC_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
     gene            complement(1082..>1328)
                     /gene="ymgC"
                     /locus_tag="ACHKUC_005118"
     CDS             complement(1082..>1328)
                     /gene="ymgC"
                     /locus_tag="ACHKUC_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO6621156.1"
                     /translation="NNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLKD
                     ETSVILSDLYLQILQYIEMHKTTLTDIIINDREFVLS"
BASE COUNT          418 a          295 c          253 g          362 t
ORIGIN      
        1 aaactttcac acaattatag caaacaaaag aatgaacaat agtaacaaga ggcaacgagc
       61 taattagctg tctataatta caccgcacca tggccttgct gatcaatttt cactcaacca
      121 caaccttcac cttcggttta gaaaggataa ttttgaccat gtcgatatat gtaacaggtt
      181 tatagaaata ataaccctgc tgaaatgtga tattattttg gttcagatag tcaaattgtt
      241 ctttcgtttc gacaccttca gcgacgatac tgatcgaaag tttacgcgcc agatccaata
      301 cacaatccag aattcgggtt gatgctgggt ctgcgttgac gcggccaacg aaactatgat
      361 caattttgat ataatcaata tgcaggtcat gcagataaga aagccctgag taaccagtac
      421 cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag aatgttcaac cgctgaacca
      481 gactttcatc aacatttaat ggctcacgct cggtgacttc cagaacaagg tttaaatcgc
      541 ggcgggtaaa actgtcacgg taatttaagc actcgtcgac aaacgtcggc gaaataatat
      601 gcgaggcgct aaaattaatc ccaatatgaa aaccttccgg cagtttactc gcgatagcgt
      661 tcatctgtct ggcaacctga ttcatcaggc tttgcgtaag cggaacgatt aatccgcatt
      721 tttcagcaag tggaataaat gccgcgggtg atatatatcc accgtgaggt tgtttccagc
      781 gggctaacac ctcaactccc cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa
      841 aagggacgat ctccccatta tttatagccc gtcgcaggat ctcttcaggc gttgcgcttt
      901 tataggcaga attaaacaaa ttaggcggga tatcaggcgt caagaatgga aaacgagcgc
      961 tctccattcc tgtttgccct ctgatattgc ggatatatta agaaagcata acgcctgaaa
     1021 tgctcacttt gcatcagcat agtgatacag ctgatgttta ttcaaaaacc ttactcaagt
     1081 tttaagagag cacaaattcc ctgtcattaa taatgatatc cgttagtgta gtcttatgca
     1141 tttcaatgta ctgcaagatt tgcagataga gatcagaaag aataacactg gtctcatctt
     1201 ttaaacgcaa gcgtaaagcc tgagcaataa tatgactatc aacatatcca tgtgtcatga
     1261 acacctccct ctcgagattt ttaaacaaag cacattgaaa aataaatctc tctgggattg
     1321 aattattc
//
LOCUS       DAZFRG010000494         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFRG010000494 DAZFRG010000000
VERSION     DAZFRG010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.497"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..1260
                     /gene="frc"
                     /locus_tag="ACHKUC_005119"
     CDS             10..1260
                     /gene="frc"
                     /locus_tag="ACHKUC_005119"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO6621157.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT          353 a          291 c          330 g          351 t
ORIGIN      
        1 ggtattgcta tgtcaactcc acttcaagga attaaagttc tcgatttcac cggtgtgcaa
       61 tctggcccat cttgtactca aatgctggcc tggtttggcg ctgacgtcat taaaattgaa
      121 cgtcccggcg ttggtgacgt aacgcgccac cagctgcgag atattcctga tatcgatgcg
      181 ctttacttca ccatgcttaa cagtaacaaa cgttctattg aattaaatac caaaacagcg
      241 gaaggcaaag aggtaatgga aaagctgatc cgcgaagctg atatcttagt cgagaacttt
      301 catccagggg ccattgatca tatgggcttc acctgggagc atattcaaga aatcaatcca
      361 cgtctgattt ttggttcgat caaagggttt gatgagtgtt cgccttatgt gaatgtaaaa
      421 gcctatgaaa acgttgctca ggcagcgggt ggcgcggcat ctactacagg tttttgggac
      481 ggtccgccgc tggtaagcgc tgcagcgttg ggtgacagca acacaggaat gcatttgctg
      541 atcggtttac ttgctgcttt gctgcatcgc gaaaaaacgg ggcgtgggca acgagtcacc
      601 atgtcaatgc aggatgccgt gttgaacctt tgccgcgtga aattacgcga ccagcagcgt
      661 ctcgataagt tggggtatct ggaagaatac ccgcagtatc cgaatggcac atttggtgat
      721 gcagttcccc gcggtggtaa tgcgggtggt ggcggtcagc ctggctggat cctgaaatgt
      781 aaaggctggg aaaccgatcc taacgcctat atttatttca ctattcagga gcaaaactgg
      841 gaaaacacct gtaaagccat cggcaaacca gaatggatta ccgatccggc atacagtaca
      901 gcccatgccc gacagccaca tattttcgat atttttgctg aaatcgaaaa atacactgtc
      961 actattgata aacatgaagc ggtggcctat ttgactcagt ttgatattcc ttgtgcaccg
     1021 gttttaagta tgaaagaaat ttcacttgat ccctctttgc gccaaagtgg cagtgttgtc
     1081 gaagtggaac aaccgttgcg tggaaaatat ctgacagttg gttgtccaat gaaattctct
     1141 gcctttacgc cggatattaa agctgcgccg ctattaggtg aacataccgc tgctgtattg
     1201 caggagctgg gttatagcga cgatgaaatt gctgcaatga agcaaaacca cgccatctga
     1261 tattaacggg ggcttctggc tcccaattta taaaaatttc gaggttatta atcatgtcag
     1321 atcaa
//
LOCUS       DAZFRG010000495         1307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFRG010000495 DAZFRG010000000
VERSION     DAZFRG010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.498"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHKUC_005120"
     CDS             complement(<1..296)
                     /locus_tag="ACHKUC_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO6621158.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSV"
     gene            complement(447..644)
                     /locus_tag="ACHKUC_005121"
     CDS             complement(447..644)
                     /locus_tag="ACHKUC_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6621159.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(871..>1307)
                     /locus_tag="ACHKUC_005122"
     CDS             complement(871..>1307)
                     /locus_tag="ACHKUC_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6621160.1"
                     /translation="VFTNKVQYPDGKPPVWAKCTKGVYPSYWEEWKKVREVVKVACPE
                     CGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGCERLPSTEAFNAIC
                     KVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
BASE COUNT          342 a          379 c          264 g          322 t
ORIGIN      
        1 acactgaagc gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac
       61 aggtaaagac ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc
      121 cacttcaggt aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta
      181 aagtacggcg ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat
      241 tccaggcagt cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat
      301 cattaaacct gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt
      361 tgcttgtgat ccagacgcat gagcagatgg ctggtgagtg cccctaacac ccaccagccg
      421 cccatttacc acaaataaaa aagccttcag gactgaaggc gtctgtaaca accaaactga
      481 tagtctgcca gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa
      541 aggtaagtat tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta
      601 aacaccactc tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt ttcccttttc
      661 cggttaacgt gacacaccaa taactcttgt cgaaaaagcc agcaagctga aagacaggta
      721 ttcaccgcca ccagcgcgtt tactgtactg acgcgatttc agtcataaaa aacccgccag
      781 gcggcggggt gtaaaaaatc ttctaacgtc aggcataaaa cgcccatcgt tagagcaaat
      841 ttaccacaga ttcgggaaaa atcaacaaca ctatcgcgtt accctcttta actgccgctc
      901 cgcccatgcc tcttcaatgt caaaccgaac caccaacgta tcgtaaaagc gtttcactga
      961 ttttttccac gtatcaagcg tgatagcact cgtcactttg catatggcat taaatgcctc
     1021 cgttgatggc agcctttcac agccacgacc accacaacgc tggcaatctc tgataacagg
     1081 cataccacgt tttaccgact cttcacgatg aatggcgaca ccacgcccac ggcaatcctt
     1141 acaggcggtg gaaacctcac cctttccgcc acactccgga caggcaactt ttaccacctc
     1201 cctgactttt ttccattctt cccagtaaga cggatacaca cctttcgtac actttgccca
     1261 taccggcggc ttaccatccg gatactggac cttgtttgta aaaacta
//
LOCUS       DAZFRG010000496         1306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFRG010000496 DAZFRG010000000
VERSION     DAZFRG010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.499"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..194
                     /locus_tag="ACHKUC_005123"
     CDS             <1..194
                     /locus_tag="ACHKUC_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621161.1"
                     /translation="PYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAP
                     GLKKSLGDVTKAAFDLCDK"
     gene            200..1201
                     /locus_tag="ACHKUC_005124"
     CDS             200..1201
                     /locus_tag="ACHKUC_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6621162.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
BASE COUNT          377 a          220 c          335 g          374 t
ORIGIN      
        1 atccttatgt gatgaatttt gacgataata agttatatgc acttacaggt ttggctcgaa
       61 taaaaggtaa aacatttggt gcgttaccgt tagataacaa taatccattt tggctagata
      121 atgatgccgc cccagggtta aaaaagagtc tgggggatgt cactaaggct gcatttgatt
      181 tatgtgataa gtaactaaaa tgtcggttcg taagattcca tcaggtaaat ggctttgcga
      241 atgttatccc tacggggcat cgggaaaacg cattcgtaaa cagtttgcga caaaaagtga
      301 ggcgctctct tatgagcgcc gtttaatgaa tagtagagtt ggagacgagt ttcaagatgg
      361 ttctggtcct cgtctttctg agttgattgc tcgttggttt gagatgtacg gtaaaacctt
      421 gtcctctggt gcagagcgca aagtcaaact tgaggcgatt tgttccaggc tgggagatcc
      481 atttgcttct cagtttgaca aaaatatgtt tgctacttat cgggaaagaa ggctatcagg
      541 agaatggaat cccaagggga agaaaaaact tagtgaagca accgttaatc gcgagcagtc
      601 atatctacat gctgtttttg ccgaactgaa gcgccttggg gagtggtctg gtgaaaaccc
      661 cctgactggt attcgcaagt ttcgtgagga agaaaaggaa ctggcgtttc tgtatgtaga
      721 tgagattgaa cgccttctga ttgcgtgtga tgagtcacgg aataaagatt tgggggttgt
      781 tgtccgtatt gggcttgcga cgggtgctcg gtggagtgaa gcagaaggat taaagcaatc
      841 tcaagtactg cccggtcgaa tcacatttgt taaaactaaa ggaaagaaga accgcactgt
      901 accgatttca cctcaattgc aggctatgct tcctaaaaaa cgaggagcgc tattttcacc
      961 atgttatgag gcttttgacg ctgcaattaa gagagcgaag atcgagcttc ctgatgggca
     1021 attaactcat gtgctacgtc acacgtttgc cagtcatttt atgatgcggg gcggaaatat
     1081 tcttgtgttg caaaaaatac tggggcatag cgatataaaa atgactatgc gttatgcgca
     1141 ttttgctcca ggtcatttag aggctgctgt tgaattgaac ccttttgaca atagagggta
     1201 aaaagtggcg ataaaaagtg acaatagatt caaatggcgt tgtatagagc catttccaaa
     1261 gttaataaaa tcataaggtt atgatttata aatggtggtt gatagt
//
LOCUS       DAZFRG010000497         1299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFRG010000497 DAZFRG010000000
VERSION     DAZFRG010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.500"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..243
                     /locus_tag="ACHKUC_005125"
     CDS             55..243
                     /locus_tag="ACHKUC_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6621163.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDSDEDKTDSAALLPC
                     IEQARQDVRTLRHLHQNR"
     gene            328..1175
                     /locus_tag="ACHKUC_005126"
                     /pseudo
     CDS             328..1175
                     /locus_tag="ACHKUC_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          383 a          313 c          298 g          305 t
ORIGIN      
        1 ctgaatgcct gccggattta cccactttcg actggtaacc cggaaattca caacatgaaa
       61 tcattaacca cggaaacagc gctggatatt ctgattgcgt ggctgcagga caatattgac
      121 tgcgaatcag ggattatctt cgacagcgat gaggataaaa cggattcagc agcactgctg
      181 ccctgcattg aacaggcccg gcaggatgtc cgtaccctgc gtcatctgca ccagaaccgg
      241 tgagtctcac tcatcatact tcattccact gacgccagcc tgaacagggc tggcgttttc
      301 atttatctgc aaaaaggaat atcgattatg tctgatatca cagcctccgt ccggaagtgg
      361 tcaacgggca tacggaagtt atctgctcaa ccagtatcca ccacattctg gctgtacgaa
      421 aaagtacact gctgcaaatc gacacactta tccggcaact ggctgaaatc tcagttctga
      481 cggaaagcat tggaggtaaa accacccggg actgggccat gaaacaggat tttcgctgcg
      541 gctgctggct catggagaaa cctgaaacgg caatgaaagc catcacccgc aaccttgacc
      601 gtgaaatctg gcgtgacctg atgcaacgtt ccaggatgct ttccttaatg gatgcgcagg
      661 cccgtgatac atggtaccgg tcactggagt acgataattt tccggaaatc agcgaagcga
      721 acattctgag cacatttaaa caactgcacc agaacaagga tgaggtgttt gagcggggag
      781 tgatcaacgt cttcagggga ctgagctgga attataaaac caactctccc tgtaaatttg
      841 gaagtaaaat cattatcaac aatctggtga aatgggaccg gtggagattt catcttatca
      901 ccggacaaca gactgaccga ctcgccgatc tggaaagaat gctacatctg ttcagcgaca
      961 aaccgattcc cgacaaccgg gaaaatatca tcattcggct ggatgatcac atccgctccg
     1021 ttcagggcaa agtgtgctat gaagatgaga tgttcaacat cagatacttt aagaagggct
     1081 ccgcacacat cacgttcagg aaaccagaac tggttgatcg gctaaatgat attattgcga
     1141 aacattatgc gggaaaagta tcttcttcag tatagactta tatatcacag gtattgtgtc
     1201 tcgtccggaa caagacaggt aggtatgctg cattcactga accattacag agaatatttc
     1261 aacagagttc aatatacctc catgtataac aactacacc
//
LOCUS       DAZFRG010000498         1277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFRG010000498 DAZFRG010000000
VERSION     DAZFRG010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.501"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..1244
                     /gene="ugd"
                     /locus_tag="ACHKUC_005127"
     CDS             78..1244
                     /gene="ugd"
                     /locus_tag="ACHKUC_005127"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6621164.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT          365 a          275 c          272 g          365 t
ORIGIN      
        1 agcgttaaaa cttttagctc ttatcaggat gctaaaaaca tcatgattca cagttaagtt
       61 aattctgaga gcatgaaatg aaaattacca tttccggtac tggctatgta ggcttgtcaa
      121 acgggcttct aatcgcacaa aatcatgagg ttgtggcatt agatatttta ccgtcacgcg
      181 ttgctatgct gaatgatcgg atatctccta ttgttgataa ggaaattcag cagtttttgc
      241 aatcagataa aatacacttt aatgccacat tagataaaaa tgaagcctac cgggatgctg
      301 attatgtcat tatcgccact ccaaccgact atgatcctaa aactaattat ttcaatacat
      361 ccagtgtaga atcagtaatt aaagacgtag ttgagataaa tccttatgcg gttatggtaa
      421 tcaaatcaac ggttcccgtt ggttttaccg cagcgatgca taagaaatat cgcactgaaa
      481 atattatttt ctctccggaa tttctccgtg aaggtaaagc tctttacgat aatctccatc
      541 cttcacgtat tgtcattggc gagcgttcag aacgcgcaga acgttttgct gcgttgttac
      601 aggaaggcgc gattaagcaa aatatcccga ccctgtttac cgactccact gaagcagaag
      661 cgattaaact gtttgctaat acctatctgg cgatgcgcgt agcatacttt aatgaactgg
      721 atagctatgc agaaagttta ggtctgaata ctcgtcagat tatcgaaggt gtttgtctcg
      781 atccgcgtat tggcaaccat tacaacaatc cgtcgtttgg ctatggtggt tattgtctac
      841 cgaaagatac caagcagtta ctggcgaact accagtctgt gccgaataac ctgatctcgg
      901 caattgtcga tgcaaaccgc acgcgtaaag actttattgc cgatgccatt ttgtcacgca
      961 agccgcaagt ggtgggtatt tatcgtctga ttatgaagag cggttcagat aacttccgcg
     1021 cgtcttccat tcaggggatt atgaaacgta ttaaggcgaa aggcgttgaa gtgatcatct
     1081 acgagccggt gatgaaagaa gactcattct tcaactctcg cctggaacgt gatctcgcca
     1141 ccttcaaaca acaagccgac gtcattatct ccaaccgtat ggcagaagag cttaaggatg
     1201 tggcagataa ggtctacacc cgcgatctct ttggcagcga ctaacatcct gttatcaggg
     1261 cgattttcgc cctgatt
//
LOCUS       DAZFRG010000499         1276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFRG010000499 DAZFRG010000000
VERSION     DAZFRG010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.502"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1276)
                     /locus_tag="ACHKUC_005128"
     CDS             complement(<1..>1276)
                     /locus_tag="ACHKUC_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6621165.1"
                     /translation="GFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFL
                     NAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSND
                     VSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSS
                     YNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLC
                     LASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDV
                     LTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDI
                     IQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQM
                     TISIGTVFSTGDTHNISLVMTEADKALREAKSE"
BASE COUNT          428 a          246 c          260 g          342 t
ORIGIN      
        1 cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc gagatattat
       61 gcgtgtcacc cgttgaaaaa accgtgccaa tactgatggt catttgttca ggaacatcat
      121 atctgttact aaagccggtc aggagttcga catttttacg catacgttcc gcaatgattt
      181 ttgcctgggc actattgagt tcggtaaata gcaagccaaa tacctcaccc tccagtcgcg
      241 ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa accagcactt
      301 tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta atatcaacca
      361 gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg ctgttgaaaa
      421 agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg ctggagagtt
      481 gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta ctggaaactt
      541 caatcccgcg actgatatac cagatggtgt aattatactc atccagagtc agtagaagta
      601 agttgcagca tagcgatgcc agacataata atgctattaa cggccatagt tcgctggcta
      661 atcgtgtacg catgataata aagaacagta aaaatgccca caaacaaacc aatatttttg
      721 tatagttgat tccccaggtc gcagtatggt tgtccggaca gtaatcgacg acatacaaag
      781 aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact aaaaaaggaa
      841 tcagcgccag caacagaatg ccagttttct tcttattgtt gtcaaggatg ttgtctttcc
      901 cataacaaaa gagcgccagc gaggttaaac aaatgaaact gagttggcgg aaaatataga
      961 aaatagaaac atcattcgac tttgtctgga tcagtgtact gccgttgatt ggtttatgga
     1021 taattacaat ggtttcaact aaatagataa ggccacttaa aaatgcaagg cttaaaatga
     1081 gtgttggtaa aatttgtttg ttagtgaaat acttcattaa catgaaaagg aaaatgacgg
     1141 cgttaaggaa aaacaaagcc acaacaacca tcaggtaatt catctgtggg atgagtttta
     1201 catttgtatt ttcgttatat aaggtagtaa tgcaaaataa aattaacgtc agcaataagc
     1261 tgaaaataaa aaagcc
//
LOCUS       DAZFRG010000500         1273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFRG010000500 DAZFRG010000000
VERSION     DAZFRG010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.503"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..736
                     /gene="nanR"
                     /locus_tag="ACHKUC_005129"
     CDS             2..736
                     /gene="nanR"
                     /locus_tag="ACHKUC_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6621166.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(772..>1273)
                     /locus_tag="ACHKUC_005130"
     CDS             complement(772..>1273)
                     /locus_tag="ACHKUC_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6621167.1"
                     /translation="KWHLQRHRIGVLGFSAGGHIASTFVNQICDFKRSSDSKEQEIFA
                     IPDFVGLIYPVISMKDDVTHPGSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHC
                     CDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSIQWATDLSVGGWIKLFMDWM
                     YSHGFK"
BASE COUNT          444 a          268 c          230 g          331 t
ORIGIN      
        1 tatgtccgac aacaaaaaaa ccgtgttacc cgggctaaca acgaccagaa agcccattgt
       61 tacaaaaaaa ctttcagaaa tcgttgccga agagcttgaa agaatgatcc gccaggagga
      121 gataagggaa ggtgaatacc tgccttctga acgtgaactt atggcattct ttggcgttgg
      181 aagaccatct gtcagagagg ctttatcatc tctcagacat aaagggctaa tccggtataa
      241 caagggagaa aaagccagtg tctgccgtcc atcaccagaa accatcatta atgggttatc
      301 aggcattgtt aaagattttt tatctacagc tgatggcatc aaggattttg aaagactacg
      361 catctttttt gaaaccaacc ttgtaagata tgcagcaaaa aatgcaacgc ctgaggatat
      421 tcacaggctt gagaaggtgc tcagtatcca tcataactca ctgagaaccc ccgaagtgta
      481 tgctcaatat gatattgaat ttcatagtat acttgctgat atacctgaca ataaagtatt
      541 aaaaacaata cactcagcat tttctgaatg gctgataatg gccaaacctc tggaaaagaa
      601 taaaatgaca tatgaaaata acaaaaaaac ccatcagcag catattgaaa tacttacggc
      661 cataaaagag catgattgtg ataaagctga acagctaatg tataatcacc tttataaacg
      721 aattgaagca tcatagttac tcgacgcttc accaggtagt caccataaat attatttaaa
      781 tccatgagaa tacatccagt ccataaacag ttttatccat cctccaacag aaagatctgt
      841 ggcccactga atactaaaac catgccctcc tttctcatag agatgcattt cagcagaaac
      901 gcctttctct attaggccac ggtacatgag cagactgttt tcaattgaca caagatcatc
      961 atcacaacaa tggagtaaaa acacaggagg aaaacatgat gtgacattaa gatccgcact
     1021 atattgtatt atattttctg aggtactatt gtcccccagt aattgcttcc tggagcctgg
     1081 atgtgtcaca tcatctttca ttgatattac aggataaatt aacccgacaa aatcgggaat
     1141 agcgaaaatc tcttgttctt tactgtcaga agaacgtttg aaatcacaaa tctgattcac
     1201 aaacgttgag gcgatatgcc ccccagcaga aaatcctaat acaccaatac gatgacgctg
     1261 tagatgccat tta
//
LOCUS       DAZFRG010000501         1271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFRG010000501 DAZFRG010000000
VERSION     DAZFRG010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.504"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..886
                     /locus_tag="ACHKUC_005131"
     CDS             128..886
                     /locus_tag="ACHKUC_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO6621168.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(939..1046)
                     /gene="yniD"
                     /locus_tag="ACHKUC_005132"
     CDS             complement(939..1046)
                     /gene="yniD"
                     /locus_tag="ACHKUC_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO6621169.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          318 a          287 c          301 g          365 t
ORIGIN      
        1 aatacattgc taagttattc gcgccttttt ataattcgtt acctttccag ggacgctaat
       61 ctttgatcct taagaaagtt tccgggaccg cgatggtctc tttttttgtt gtacggactc
      121 ttgataaatg aagcttttga agacagttcc tgccatcgtt atgttggcgg ggggcatgtt
      181 tgcgtcactg aatgcagctg ccgatgattc cgtttttact gtcatggacg atcctgccag
      241 cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat cttgcacaat caggcaatac
      301 gaaaagctcc tcacttacgg ctgacaccac tatgacctgg tatggacaaa ccactgcctg
      361 gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat gagcgttctt cagaaaaata
      421 tgcggcgggc gggcgtagcc gttttaattt aactgattat gactatttat ttggtcaggc
      481 aagctggctg acagaccgtt ataacggcta tcgagagcgc gatgtgttga ccgcgggtta
      541 tggtcgccag tttttgaatg gcccggtaca cagtttccgc tttgaattcg gccctggtgt
      601 gcgttacgac aaatataccg acaatgccag tgaaacccag ccgctgggct atgcctccgg
      661 ggcttatgca tggcagttga ctgacaacgc taaatttacg cagggtgttt cggtctttgg
      721 cgcggaagac acaacgctga actccgaaag cgcattaaat gttgctatta atgaacattt
      781 tggactgaag gtggcttaca acgttacatg gaactctgaa cctccagagt ctgcaccgga
      841 acataccgat cgtcggacaa ccttatcgtt gggttacagc atgtaaaatt atctgccgga
      901 ccgtgttatg cggtctggct gttttcgttt gtcactgctc agccccaaat cattgcagcc
      961 caacgtagca acaacatagc gccacagatt gccagtagca ccaccaccat cttccagtgt
     1021 tttttatcaa agcgtttagt cggcatacca gtgtcctgca tatctcttgt gcgtgataac
     1081 ggagtgtaac aaaagggaca ggcatcaagg gattttttgt tattacagtc gtcgggtggc
     1141 gttgtacacc tggtcactgt ttctgcattt tatgaaaaaa attatgtatc gatatacaaa
     1201 tatcataaat agcgggttat aaatacgtct ttagttcatc agcactaaaa ctcaggcttt
     1261 ggctgattac g
//
LOCUS       DAZFRG010000502         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFRG010000502 DAZFRG010000000
VERSION     DAZFRG010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.505"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1269)
                     /locus_tag="ACHKUC_005133"
     CDS             complement(<1..>1269)
                     /locus_tag="ACHKUC_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO6621170.1"
                     /translation="SVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFK
                     NYLQQHPGFEDFFIAVDPQTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDL
                     NYRSPEVLLAMVDILLCYLEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSI
                     IDNVAPGTVIITETNVPHKDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCA
                     WAQNLTLPSSNTTWFNFLASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNP
                     DGTRSPYEINVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRND
                     YAGVEKLGYNRAINRKKYSSKEITTELNNKATLRYSIYYKLSRLITLRCSHNEFHPDN
                     DFTIDTVNSSVMCIQRSNADGNCLTGLFNVS"
BASE COUNT          337 a          306 c          274 g          352 t
ORIGIN      
        1 actgacatta aacaatcctg tcagacaatt accatccgcg ttgcttcttt gaatacacat
       61 tacggatgaa ttaaccgtgt cgatggtaaa atcattatcc ggatgaaact cgttatgact
      121 gcaacgaagt gtaattaaac gcgacaactt ataatatatc gaatacctta atgtcgcttt
      181 attgttcagt tctgtcgtta tctctttact agagtatttt ttacggttaa tcgcacgatt
      241 atatccaagt ttttcgacac ctgcgtaatc attacgcgac cccagaatgc tttgaatata
      301 tatcgctggc acaccgggga aacttaacaa aatcgcatgg gcgagaataa acctggcaca
      361 ccgttcttca tcgctactct cgcggcggct taacgcatcc atataggtga catttatttc
      421 atacgggcta cgcgtaccgt cgggattatt tttccagttt actaatgcac cttcctgctg
      481 taatgcctcg accagcgcta atatttcgct ttctggtaac aagccacgca gtgggttcag
      541 cccgatgcca tcgtgagagg cgaggaagtt aaaccaggtg gtgttgctgg aaggtagtgt
      601 caggttttgc gcccacgcac aaagcgcctc aacgttctgt ttttgcaccg catgcagcac
      661 cagcggcggc agcgagaact ggtacaccat atgtgcttcg tcatcgcctt cgccaaagta
      721 agcaatgttg tctttatgcg gaacattggt ctcggtaatg atcactgtgc ctggcgcgac
      781 gttatcaata atcgaccgta acagtttgat aatcagatgt gttttttcca gatggatgca
      841 gcttgttccc ggctctttcc acataaagcc aacggcatcc agacggacgt attccgcgcc
      901 cttttccagg taacacagca atatatccac catcgccagc aacacttcag ggctacggta
      961 attgaggtca atttgatcgt cgctaaaggt ggtccataaa tggcgcgtgg aattatcatc
     1021 catttgaaac ggtgttaata gcggtaacgc acgcggacgg gtgacggcgc tgagatcggt
     1081 ttgcggatca acggcaataa aaaaatcttc aaaacctgga tgctgttgta aatagttttt
     1141 aaaccattca ctttttgccg acatatggtt gcagacaaaa tcaaacatta aatgactgca
     1201 ttcaccgagt tgctgaatat cctgccactc ccccgcttca ctggcgacct gatgataatc
     1261 aattaccga
//
LOCUS       DAZFRG010000503         1256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFRG010000503 DAZFRG010000000
VERSION     DAZFRG010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.506"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1252)
                     /locus_tag="ACHKUC_005134"
     CDS             complement(<1..1252)
                     /locus_tag="ACHKUC_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6621171.1"
                     /translation="MSGISVVSASVVNNLLPYQIYRDFAENKGDFIPGTESLELYNNS
                     GLLVTTLNKAPMPDFSSVDRLLGVATLVNPQYVVSVKHNGGYSRVRFGTSNYTLVDRN
                     NYSARDFHTPRLNKLVTDVVPVEVTSAGTAQGTYKNVTKFPVFYRIGTGTQIYKNEQG
                     YYTLNGAYSYLTGGIVPTPSISDWSFVTNAGNGALASYGTPGDSGSPLLGWDTTLNKW
                     ALVGVLRAYAGTTGSTNWYVVIPTVQITANINADTDKPVTSEAGSGDILWTFNSVTGT
                     GQLVQGDNSWDMHGQKDLDLNYGKNLTFSGGGTIVLENSVTQGAGALTFNDDYVVKPL
                     DDQTWIGGGVIVNDGHVVDWQVNGVSGDALHKLGTGTLKIDGSGINGGSLNTGDGAVI
                     LAQRPDGEGKVQAFSRVSLVSGRSI"
BASE COUNT          362 a          330 c          219 g          345 t
ORIGIN      
        1 caatagagcg accactaaca agagataccc tactaaaagc ctgaactttc ccctcgccat
       61 cgggtctctg agccagaata actgcgccat caccagtatt caggcttcca ccattaatcc
      121 cggagccatc aattttcaac gtacccgtgc ctaatttatg cagcgcatct cctgacacac
      181 cattgacctg ccagtcaaca acgtgcccat cattgacaat gacacccccc ccaatccagg
      241 tctgatcatc cagaggtttc accacatagt catcattaaa agtcagcgcc ccagccccct
      301 gagtcaccga attttctaaa acaattgtac cgcctccaga aaatgtcaga tttttcccat
      361 aattgagatc taagtccttc tgaccatgca tgtcccagga attatctccc tgaactaact
      421 gaccggttcc tgtcacactg ttgaaagtcc agagaatatc accgctcccg gcctcactgg
      481 taacaggttt atcagtatca gcatttatat ttgcggtgat ttgcactgtt ggtataacaa
      541 cataccagtt ggtactccct gtggttcctg catatgctct gagtaccccc acaagagccc
      601 atttatttag agttgtatcc catccaagta gcggtgaacc actgtcaccg ggagttccat
      661 acgacgcaag agcaccgttg ccagcatttg tcacaaatga ccaatctgaa attgatggcg
      721 taggaacaat ccccccagta agataactgt aagcaccgtt cagagtataa tatccttgtt
      781 catttttata tatttgtgtc cctgtaccta tcctatagaa aacaggaaac ttggttacat
      841 ttttatatgt tccctgcgct gttcctgcac tggtaacctc tacaggaacc acatctgtta
      901 ccagtttatt taacctaggc gtatggaaat ctcttgctga gtagttattt ctgtctacta
      961 aagtataatt actcgtacca aaccgtactc tactatagcc accattatgc ttaacactga
     1021 cgacatattg tgggttaaca agtgtggcaa cgcccaatag cctatccaca gatgaaaaat
     1081 caggcatggg agctttattt aacgtagtca ctaataatcc tgagttatta tacaactcaa
     1141 gactttccgt tccaggaata aagtcgccct tattttcggc aaagtctcga tatatctgat
     1201 atggaagcaa gttattaaca acagatgctg atacgacaga gataccagac aaatta
//
LOCUS       DAZFRG010000504         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFRG010000504 DAZFRG010000000
VERSION     DAZFRG010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.507"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1255
                     /locus_tag="ACHKUC_005135"
     CDS             <1..>1255
                     /locus_tag="ACHKUC_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6621172.1"
                     /translation="TKASEKNAKSSQTAAKTSETNAKDSEANAKVSETAAANSAKASA
                     ASQTAAKASEDAAREYANQTAEPYRYVLQPLPDVWIPFNDSLDMITGYSPGYKKVKIG
                     DNVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQRTNFFPNST
                     DPSKWNKSTSLDVTETGTDSFGFNYGRFVVQDSIVGTSKAHTIIGLYSSTGGVDTSGD
                     EKHVTISCRVKSEVDNIAVRILFEHYDGEVRTSIGAANLNLTTRIISKTGQTSRVTAR
                     SVKDDATGWIFFEATLKADTTENTVGGFVQYSPDTGQMVASGDYLDVTTPQIEAGTGA
                     SSFIVTGTAPVTRASDMVTVPIKNNLYNLPFTVLCEVHKNWYKTPNAAPRVFDTYRHQ
                     ADAGIVMGFGSSGGYDGFPYCDIGGS"
BASE COUNT          379 a          245 c          332 g          299 t
ORIGIN      
        1 cacaaaggcc agcgaaaaaa acgcaaaatc cagccagacg gcagcgaaaa ccagtgagac
       61 gaatgccaaa gacagtgaag ccaacgcaaa ggtgagcgaa acagcggcgg cgaactcggc
      121 gaaagcatcg gcagcaagcc agacggcagc aaaagcaagt gaagatgctg ccagagaata
      181 cgcaaaccag acagcagagc cgtacagata tgttttacag ccgctgccgg atgtgtggat
      241 accctttaat gattcgctgg atatgattac gggctattct ccgggttata aaaaagtgaa
      301 gattggtgat aatgtggttc aggttgccag tgataaacag gttaatttca gtcgcgcatc
      361 aacggcaaca tatatcaaca aatctggcga actgaaaacg gcggaaatta atgagccacg
      421 atttgagtgt gatggcctgc ttattgaggg gcaaagaacg aacttcttcc cgaacagtac
      481 agacccttcg aagtggaata agtcaacttc actggacgtt acagaaacag gcacagatag
      541 tttcgggttt aattatggcc ggtttgtcgt acaggattcg attgttggta caagtaaagc
      601 gcataccatt atcggactgt attcgagtac cggaggggtt gatacttcag gggacgaaaa
      661 gcatgtaact atatcctgtc gggtaaaaag tgaagttgat aatatcgccg ttcgtatttt
      721 atttgaacat tatgatgggg aggtaaggac atcaatagga gcagcaaacc tgaaccttac
      781 cacccgcata attagcaaga caggtcagac aagccgtgtt acagcaaggt ctgttaagga
      841 tgatgcaact ggctggatat tttttgaggc tacattaaaa gcagatacaa cagaaaatac
      901 ggttggtggt tttgtccagt attctccgga tacagggcag atggttgcat caggggatta
      961 tctcgatgta accactccac agattgaggc tggtacaggc gcatcatctt ttattgttac
     1021 ggggacggca ccggtaacgc gggcaagcga tatggttaca gttccgatta agaataatct
     1081 ttataatctt ccttttacgg ttctttgtga ggtacataag aactggtata aaacgccaaa
     1141 tgcagcaccg cgtgtttttg acacataccg gcatcaggca gatgcgggga tcgtaatggg
     1201 gtttggttca tcaggtgggt acgacggttt tccgtattgt gatataggtg gttca
//
LOCUS       DAZFRG010000505         1251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFRG010000505 DAZFRG010000000
VERSION     DAZFRG010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.508"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1224
                     /locus_tag="ACHKUC_005136"
     CDS             <1..1224
                     /locus_tag="ACHKUC_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO6621173.1"
                     /translation="QLKKMTHPVTIRTTDIANFWHYLESATLPVITKSTTTENREVTF
                     LWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLTLVLPASYRGSYSFIEIP
                     TDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVLSLDMAPEQKEWDDTSHK
                     CTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAETWFDRIDITRALDMAIT
                     TGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQLYRDYPDIVWAGRSNTV
                     LAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSPILFTENNTSWVSEQILS
                     APPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTVYTGGHDYAWWRGAIIDE
                     LANYNCRKVSNNNSV"
BASE COUNT          383 a          267 c          256 g          345 t
ORIGIN      
        1 cagttaaaaa aaatgacaca tcctgtcaca ataagaacaa cagatattgc taatttctgg
       61 cactatcttg agtcagcaac acttccagtg atcacaaaaa gcaccactac agaaaatcgg
      121 gaggttacat ttctgtggcg ctcagagaaa gcactgcaag gcgtatatct tcgcctgaat
      181 cgtgttacag ataaaaaaga cgtcaaaaaa ggactaatga ctcatatccc ttcgacagat
      241 atctggatgc tgacactggt gttaccagct tcatatcggg gctcatactc atttatagaa
      301 attcccacag atatgacaca aaaagacata tttcaactag gaagtcgctt ctctccatta
      361 cccggtaaat ctgatccatt taacaaaaca gcagaaataa atatacgagg attcggagaa
      421 tcagtccttt ctcttgatat ggctcctgaa caaaaggaat gggatgatac ttcccataaa
      481 tgtacaggta ttctttcaac attacattcc tttgttgcag gatatcaacg ccggattcgt
      541 ttatatttcc ccctgaatcc aacatcagta cctcttggat tacttgtgtt acctgatgct
      601 gaaacatggt ttgaccggat agatattacc cgggcattag atatggccat caccactggt
      661 cgtatcacgc caatggcaat tatggggata gacaatatta atgaatctga tcgtatgaat
      721 atactgggag gcaataaaga actcatcttt gatatagcgg aaaatctgat accccagtta
      781 tacagagact atccggatat cgtatgggct ggtcgctcta atactgtact ggccgggcag
      841 agcctcggtg gagtgacagc actgatggca gctatatatg cgtcgacaac atttggtaca
      901 atcattagtc actcaccttc aatgtggtgg aaccctgacc agggcagccc gatcttgttt
      961 actgagaata atacctcctg ggtaagtgag cagatacttt cagcgcctcc gaaagatgta
     1021 aatatccgac ttggagtcgg ttctttagaa ggtacaaccg tctcacatgt tcagcgattg
     1081 catcattcgt taatcgcagc tggtttggaa agtaacctca ctgtctatac cggtggtcac
     1141 gattatgctt ggtggcgcgg agcaattatt gatgaattag caaattataa ttgcaggaag
     1201 gtatcaaata ataactctgt gtaaaatatc gttttatcgt ccgataactc t
//
LOCUS       DAZFRG010000506         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFRG010000506 DAZFRG010000000
VERSION     DAZFRG010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.509"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..347
                     /locus_tag="ACHKUC_005137"
     CDS             <1..347
                     /locus_tag="ACHKUC_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6621174.1"
                     /translation="TLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRG
                     RFSDADAYHPDQAFPLLMKQPELMLTSGELNPRHQHTVTLYAKGLTCEADTLGSCDYV
                     YLAVYPTPEMKN"
     gene            436..813
                     /locus_tag="ACHKUC_005138"
     CDS             436..813
                     /locus_tag="ACHKUC_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6621175.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            810..>1238
                     /locus_tag="ACHKUC_005139"
     CDS             810..>1238
                     /locus_tag="ACHKUC_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6621176.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVS"
BASE COUNT          288 a          355 c          319 g          276 t
ORIGIN      
        1 caacgcttcc cgacgacaac aaagacctcc cctggtgggg gctgccctgc accgtgacgc
       61 cctgtttcgg ggcctgtctg gtgcaggagg gtaaccggtt gcattacctt gccgaccgcg
      121 ccggtatcag aggccggttc agcgatgcgg atgcgtacca tccggaccag gcctttccgc
      181 tgctgatgaa acaaccggaa ctcatgctca ccagcggtga actgaatccc cgccatcagc
      241 ataccgtcac actgtatgcg aaaggactga cctgcgaagc cgataccctg ggctcctgtg
      301 attacgttta tctggctgtt tatccgacgc ccgaaatgaa aaattaactc tccagaatag
      361 ccttctgcta cggcctggtg ttttcaccac gccacttttc catttttata tctgcatatc
      421 aggaaaatct tcagtatgaa aacattaccc gacacacacg tacgggcggc atcgcgctgc
      481 ccgtctcccg tcaccatctg gcagacactg ctcacccgac tgctggacca gcactatggc
      541 ctcacgctga atgacacacc gttcgctgat gaacgtgtga ttgagcagca tattgaggca
      601 gggatttcac tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgca ggtacgtacc
      661 gaccagccgg gattcagcgc aggagccccg tcgcagttaa tcaacagcat tgatattctc
      721 cgggctcgca gggcgaccgg cctgatgacc cggaacaatt acagaatggt aaataacatt
      781 acccagggca agcatccgga ggcaaaacga tgaaactggc cctgacgctg gaagctgaca
      841 gcgttaacgt acaggcactg aacatggggc gaattgtcgt tgacgtcgat ggtgttgagc
      901 ttgccgaact gattaacgtg gtctgcgata atggctactc ccttcgtgtt gttgatgaat
      961 ctgaccggac ctcaacagac agcatacccc cctctgctgc cctgagcggt atacgctgta
     1021 gtaccgcgca tatcacggaa acagacaacg cctggctgta ctcgctgtcg catcagacga
     1081 atgacaccgg cgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg
     1141 atgcgtggtc atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgcc
     1201 gtctggttgt cacacttacc cgacgttatg gcgtcagt
//
LOCUS       DAZFRG010000507         1218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFRG010000507 DAZFRG010000000
VERSION     DAZFRG010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.510"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..1165)
                     /locus_tag="ACHKUC_005140"
     CDS             complement(14..1165)
                     /locus_tag="ACHKUC_005140"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO6621177.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          269 a          287 c          270 g          392 t
ORIGIN      
        1 cgcggttgta gattcaatct gtcaatgcaa cacccctttc aattatctct ttcggtgttt
       61 tgaacttcag tgtctttctc ggtctgttgt ttagctgagc agcaaccaga tctagttcat
      121 gttgagtata ttgggcaaga catgtctttt taggaaagta ctgccgaatt agcccatttg
      181 tgttctcatt tgttccccgc tgccaaggac tctgaggatc gcagaagtaa actttaacgc
      241 cggtgctgac agtaaattct agatgtctgg ccagttccat tcctctgtcc catgtcagtg
      301 attttctgag ttctgacggt aaactcagga atttgtcggt aagagcctga tttactgaga
      361 cagaatcttt gcccctgagt ctaaggatga tcgtataacg tgattttcgg tctacaagtg
      421 tggctatatg agagtttttt gtacctgaga ctaaatcgcc ctcccaatgt cccagagagc
      481 gtctgttatc gatatttcgg gaacgttcgt gaattggtgt tccgttcact atgttaatcg
      541 tacctctttc gcctttgcgg gtatgacgcc tgccatggcg aaggctatgc gaccgtcgca
      601 gatgctgtat attcaggtgg tgtagcgctt cacggctacg aaagtacagc gttttataaa
      661 ttgtctcagg tgatattcgc agcgtttttt gacgtggttt tgttcgcctt aaccatcctg
      721 atatttgctc tggagaccat ttcatctcca gcttttccag aacaagcttt cgcaatggta
      781 aattttgatc cagtaagcac ggttttggcc ttttcgccat tctgttggct cggttattag
      841 catcaacagc tttgtaatag cgtctgcccc gattacgctg aacttcacgt gagatcgtcg
      901 aaggactgcg attcagcgca gtagctatcg cacgaatgct cattttggct gacaaaccag
      961 ctcgtatctc ctcgcgctca gacagtgtca ggtgagctac agcccgctta cgctcatggg
     1021 gttttatgcc gccagtatcc cttaacatag tgaagatcgt tccgggtttt gaacccagga
     1081 tattcgctat ttcactgaag cctgttccgt tcttccatag ttcaaaaaca gaggcttttt
     1141 cctctgctgt aaatgttcgt ctcattcaaa aaacctccgc aaccccatgt tttcacataa
     1201 ctgttgcgtt gaccaatt
//
LOCUS       DAZFRG010000508         1214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFRG010000508 DAZFRG010000000
VERSION     DAZFRG010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.511"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..341
                     /locus_tag="ACHKUC_005141"
     CDS             <1..341
                     /locus_tag="ACHKUC_005141"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6621178.1"
                     /translation="QDVEITRVYTMNNTDRHQDHIAVYQASMVACRYIPQILGYETPS
                     TCLSFLPQVFESVDEVHFNKKILALQKHKSQIHRRYIQPEYIIAQAKFRGQQVSHSMC
                     EGFVVHKMVL"
     gene            355..>1214
                     /locus_tag="ACHKUC_005142"
     CDS             355..>1214
                     /locus_tag="ACHKUC_005142"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6621179.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVG"
BASE COUNT          322 a          258 c          297 g          337 t
ORIGIN      
        1 cccaggatgt ggaaattact cgagtttaca ccatgaataa tactgacagg catcaggatc
       61 acattgctgt ttatcaggca tcaatggttg cctgtcgtta tattcctcaa atactaggat
      121 acgaaacacc aagtacatgt ctgtcatttt tacctcaagt ttttgaatct gtagatgagg
      181 tccattttaa taaaaaaata ttagcattac aaaagcataa aagccagatc catcgaagat
      241 acatacagcc agaatacata attgctcaag ccaaattccg aggacaacag gttagtcatt
      301 ctatgtgtga aggttttgtt gtccataaga tggtgttgta acggagtaat agaaatgaaa
      361 actttgttat ttatcttcat gaccatagca atgttgctat ggtttttaag tacgctaaga
      421 cgtaaaccct gtcaaaagaa aggctgtatt gacgccatta taccagctta taacgaaggc
      481 ccgtgtctgg cgcagtcact ggataatctg ctgcgtaacc cttatttttg ccgggtaatt
      541 tgcgttaacg acggatccac ggacaatacc gaagcggtca tggcggaagt caaacgcaaa
      601 tggggcgacc gctttgttgc cgtcacgcaa aaaaacaccg gtaaaggtgg cgcactaatg
      661 catggactaa aatacgccac gtgtgatcaa gtatttttaa gtgatgcaga tactttcgtt
      721 ccacctgata atgatggaat gggctatatg ctggcagaaa ttgagcgcgg cgctgatgcc
      781 gttggcggaa ttccctccac cgcgttaaaa ggggcaggtc tgctgccaca tatccgcgcc
      841 accgtaaagt tgccgatgat tgttatgaag cgcacgctac agcagtttct gggcggtgca
      901 ccgtttatta tcagcggtgc ctgcgggatg ttccgtactg atgtattgcg taagttcggt
      961 ttctctgatc gtactaaagt cgaagacctt gatctcacct ggacgttggt ggcaaacggc
     1021 taccgtattc ggcaggcgaa tcgctgcatc gtatacccac aggaatgtaa cagtccgcgt
     1081 gaggagtggc gccgctggcg gcgttggatt gtgggctacg cggtctgtat gcgcctgcat
     1141 aaaagacttt tatttagccg cttcggtatc ttcagtatat ttcctatgct gttggttgtg
     1201 ctttatggcg ttgg
//
LOCUS       DAZFRG010000509         1211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFRG010000509 DAZFRG010000000
VERSION     DAZFRG010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.512"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1206)
                     /locus_tag="ACHKUC_005143"
     CDS             complement(<1..1206)
                     /locus_tag="ACHKUC_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621180.1"
                     /translation="MKRIAATSILCFSVLYSNTAFAVWPVTEEVLTPYVMGASPQSMG
                     NGMIETLVSMNDKLAQANITANENQKNSVISNANQAIQEQRMQNILKRIPDANACEEA
                     TAAGGRGSAGSNTSSAKAALAENSVKMFTGASTGINEAKNNVMGRVSIEVCSDEDVKY
                     NRGGCSAIGAYADKDKSATSLTTPPLSISDAQGNKVSNQSYDVKQMKVADAAKRNLIG
                     NLPLQQLNDRNLEDTKEGREYLSAFSSFVARSTAGTNAVDSILAIKKSGDVSVNSRGV
                     NIGSGLGSTAGASQAWTDASVKTKYATLFPGAEFPSKPSEWEMLRYEIYSRYADTTGA
                     DAWQVKISSTDEKETAHEQARMQAIDLRLQMLLIERTEDTNILLAALLGQQLQPVDKV
                     MLNNLKINAT"
BASE COUNT          299 a          281 c          225 g          406 t
ORIGIN      
        1 tgtcgcattg attttcaggt tattcaacat caccttatca acaggttgta gttgctgtcc
       61 taatagagct gctaataaaa tatttgtgtc ttctgttctt tctattagaa gcatctgtaa
      121 tcttaaatca atggcctgca ttcttgcctg ctcatgtgcg gtttcttttt catctgtact
      181 gctgatcttg acctgccatg catcagcacc agtagtatcc gcataacgag aatagatctc
      241 atatctcaac atttcccatt ccgaaggttt agacgggaac tctgctccgg ggaataatgt
      301 cgcatacttg gttttcacag acgcatcagt ccacgcctga cttgctcctg ctgtagaacc
      361 aagtccagag cctatgttta ctcctcttga gtttactgat acatcaccag atttctttat
      421 tgccagaata gaatcaacag cattagttcc cgctgtgctt ctggcaacaa aacttgagaa
      481 tgctgacagg tattcgcgtc cttctttcgt atcttcaaga ttcctgtcat ttagctgctg
      541 tagcggtaaa ttaccgatta gatttcgctt agcagcgtca gccactttca tctgtttaac
      601 gtcatatgat tggtttgaaa ctttattccc ctgagcatca gatatggata gcggtggtgt
      661 ggtgagtgag gtagcagatt tatctttatc agcatatgca ccaatagcac tacatccacc
      721 tctgttgtat ttcacatcct cgtcagaaca gacttcaatt gagactcggc ccatcacgtt
      781 atttttagcc tcatttatac cggtgcttgc tcccgtaaac attttcacac tattttcagc
      841 taaagcagct ttagctgagc ttgtgtttga tcctgccgac cctctacctc ctgctgctgt
      901 agcttcctca caagcattag catcaggaat tcgcttaagg atattttgca ttctttgctc
      961 ctgaatagct tggtttgcat tgcttataac agagttcttt tgattctcat tagcagtaat
     1021 attagcctgt gcaagtttgt cattcattga gactaatgtc tcaatcattc catttcccat
     1081 agattgaggg gatgccccca ttacgtaagg tgtcaatact tcttccgtga cgggccagac
     1141 agcaaaagct gtatttgaat acagaacaga aaaacacaaa atcgaagtcg ctgctattct
     1201 ttttattttg a
//
LOCUS       DAZFRG010000510         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFRG010000510 DAZFRG010000000
VERSION     DAZFRG010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.513"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1105)
                     /gene="iha"
                     /locus_tag="ACHKUC_005144"
     CDS             complement(<1..1105)
                     /gene="iha"
                     /locus_tag="ACHKUC_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006684920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO6621181.1"
                     /translation="MIVSASGYEKKLTNAAASVSVISQEELQSSQYHDLAEALRSVEG
                     VDVESGTGKTGGLEISIRGMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAA
                     IERIEVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFN
                     FWSSGPLVDDSVSLQVRGSTQQRQGSSVTSLSDTAATRIPYPTESQNYNLGARLDWKA
                     SAQDVLWFDMDTTRQRYDNRDGQLGSLTGGYDRTLRYERNKISAGYDHTFTFGTWKSY
                     LNWNETENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGESHLVTVGGE
                     FQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHL"
BASE COUNT          284 a          352 c          274 g          295 t
ORIGIN      
        1 tgagatgcca ctcatcctca gcaaataccg accagctttt ctgccggaaa gtttcacctg
       61 tgctggcaag gacaactccg tctttcatgg acgagctctg aaactcgccc cccaccgtaa
      121 ccagatgaga ttctcccaga ggggtaagca gtaatgaatt caggataagg ttcgattcct
      181 taagctcccg cggctgaccg gcaagccccc atttgtcgcg cttcagtaca ctgcgtacga
      241 gctcacgacc tttattttct gtctcgttcc agttcagata cgatttccat gttccgaagg
      301 tgaaagtatg atcatagcca gctgaaattt tgtttcgctc atagcgcagg gtccggtcat
      361 atccccccgt cagactcccc agttgcccat cccggttatc ataacgctgc cgggtggtat
      421 ccatatcaaa ccagagcaca tcctgcgccg acgccttcca gtcaagacgt gcaccaagat
      481 tataattctg tgactccgtg ggataaggaa tacgcgtggc tgctgtatcg ctcagtgatg
      541 tgaccgatga gccctgacgc tgttgtgtgc taccgcgtac ctgcaggctg acagaatcat
      601 ccacaagggg accactgctc cagaaattaa actggctgct gttaccccat ttgttgcttt
      661 cctgcagatt cagccctgca ttgacggaag agagccattt gtctgcattc tttctggtaa
      721 tgatattcac cacaccgccc atcgcatcag agccatacag tgtggacatc ggccccctga
      781 taacctcaat acgctcaatg gcggccagag ggggcatgaa cccggtattc atggcagaaa
      841 aaccgttggg agtcacgtca ctgcttccgc cctgacgaac accatcaatc agtatcagcg
      901 tgtaactggc tggcattcct cggatgctga tttccagccc accggtttta cccgtaccac
      961 tttcaacatc cacaccctct actgatctca gagcctccgc cagatcgtgg tactggctgg
     1021 actgcaattc ctcctggcta atcacagaaa cactggcggc tgcgttagtc agctttttct
     1081 catagccgga tgcagagaca atcatcacat cctctgcagc agctatgctg ctggctgaaa
     1141 atccgagaca gggaatgact acggaagcca gagtggttat tcgcatatta ctaatctccg
     1201 atcat
//
LOCUS       DAZFRG010000511         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFRG010000511 DAZFRG010000000
VERSION     DAZFRG010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.514"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..752
                     /gene="ais"
                     /locus_tag="ACHKUC_005145"
     CDS             150..752
                     /gene="ais"
                     /locus_tag="ACHKUC_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO6621182.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(791..>1205)
                     /gene="nudI"
                     /locus_tag="ACHKUC_005146"
     CDS             complement(791..>1205)
                     /gene="nudI"
                     /locus_tag="ACHKUC_005146"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO6621183.1"
                     /translation="TIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEE
                     ALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANRDVK
                     INEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          324 a          272 c          256 g          353 t
ORIGIN      
        1 ttatttagac tttgcatttg gcaaaatttt gaggcatttt gccggcatca taggattttt
       61 aatattacac caactgctaa ttatcgccag aaaagtcgct caatttcacg gtaattgtct
      121 ggttgcgctt gtctataggt ggagtttacg tgttagcttt ttgccgatct tcgttgaagt
      181 caaaaaaata tttcatcatt ttactggcgc tcgctgcgat tgccggactg ggtactcatg
      241 ccgcctggag tagcaatggt ttgccacgca tcgacaataa aacactggcc agactggcac
      301 agcagcaccc ggttgtcgtt ttgtttcgcc atgctgaacg ttgcgatcgt tcaaccaatc
      361 aatgcttgtc agataaaaca ggtattacgg tcaaaggtac ccaggatgcc cgtgaactgg
      421 gcaacgcttt tagtgctgat atccctgatt ttgatctcta ttccagtaat accgtccgga
      481 ctattcagtc ggcgacctgg ttttcggcgg gtaaaaagct gacggtagat aaacgatttc
      541 ttcaatgcgg taatgagatt tatagtgcaa ttaaagactt acaacgcaaa gcacctgata
      601 aaaatatcgt tatttttacc cataatcatt gcctgacata tattgctaaa gataagcgtg
      661 atgcgacatt taaacctgat tatctggatg gtttagtcat gcatgtggaa aaaggcaaag
      721 tttatctgga tggggaattc gttaaccact aaattttcaa tctgacagcc agtgatggct
      781 gtcatcattg ttaaagaaga cctttcaaac gtaacgtttt tcgggtggca acattcaaat
      841 cataatgcac cagatcttca ggttttaccc acgcgtagtc ctgaaactct tcgtttattt
      901 tcacatctcg gttggcagaa acgcagtcaa aaatcaggta aatcatataa atctcttcct
      961 tgcgaccatc tgcatacgtc ttggtgcgaa tatcatcgct gaaggtccac ggcgtgattt
     1021 ctgtcaaaag cagctgttct cccagttctt cgcgaatttc gcggcgtagt gcctcttcaa
     1081 ttcgttcgcc aggctccacg ccgccacccg aaagcgccca ttgaccgggg aaaacgccgc
     1141 gatcgtcggc cattttacac agcaaataag caccatcatt ttgaatcaaa gggcatacaa
     1201 tagtc
//
LOCUS       DAZFRG010000512         1192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFRG010000512 DAZFRG010000000
VERSION     DAZFRG010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.515"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..1178)
                     /locus_tag="ACHKUC_005147"
     CDS             complement(54..1178)
                     /locus_tag="ACHKUC_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6621184.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAAPSLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAEEPIISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPTPAEIAEVLMRKLKNNGKQL"
BASE COUNT          357 a          290 c          219 g          326 t
ORIGIN      
        1 tgaaggacat tatattactc ataacatttc tcgttgaaaa tttcagcaat acgctacaat
       61 tgtttcccgt tgttttttaa ttttctcatc aaaacctctg cgatctctgc aggagtgggt
      121 tgttcagcca tttcagcggc tacctgatct gaacagaagc gtagtgaatc atcataaagc
      181 aggcgattta ccaaatcact ggtcagtcca tgcttgtccg gaataatccc acacccacgc
      241 ttcgcaacaa tttctgcatt aacagagcga tctgcacctt ctccaaacac aatctgtggt
      301 attccactat acagcgctgt cagggtatta cctgcgccac cgtgatgaat aaatccatca
      361 gctccattaa ggaatacacc cataggtatc cagtcaacca gacgtacatt tgatggtagt
      421 tttcgtaatc cagtacgagc atttattgca agttgcaaaa tgatatcagc atcaacttca
      481 tttgctgaat ccataaccca tgaaatcagc tccagaccat caaccattgg ttttacagtg
      541 ccaaggctga tgagtaaacg tttccgatca gaatccctgt cccaccatgt ttcctttaca
      601 gcacctccgt tataaggaat atatcgcatt gagataatcg gttcttcagc atttttaaga
      661 atgctcatac ttgggggagc aacatcaatc catgctaaat cacatagtgg accgctgact
      721 ccatgccgcc tgtaagcatt gcttaaagaa cggtttaaca tctgaatatg ggcagatgta
      781 tgcgcgaatc caacagccag catcactgaa ggaattctat atttagcagc aaccaatggg
      841 cctgccggac caagcggggg atagactatt aaatctggcc tccattttcc tgcaaaatcg
      901 atgaggttat ctgccatttc atcgctaaaa aaagagaaat gaccaataat attacttttt
      961 ttcctcaact cttcctgatg gcgatatcca gcctctgaat ctaaacttgg agctgcatca
     1021 aaaacaacca gtccagcttc tgctgcttta ttcgcgaatt tgccagcgct actaattact
     1081 acatcatgtc cgattacacg aaaggcctga gccagagaaa tcaatgggta taacaaaccg
     1141 tacagcggag ggccaataaa aagaatacgt tttcgcatta aaaaagtccg tc
//
LOCUS       DAZFRG010000513         1184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFRG010000513 DAZFRG010000000
VERSION     DAZFRG010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.516"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(185..589)
                     /locus_tag="ACHKUC_005148"
     CDS             complement(185..589)
                     /locus_tag="ACHKUC_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO6621185.1"
                     /translation="MSEALKALNNIRTLRAHARETDLTTLEEMLEKLSAVVEDRREEE
                     SAVRKEQEERQAKLEAFRQKLLEDGIDPAELLAAVSSSQPKAKSTRTPRPAKYKYTDE
                     NGNEQTWTGQGRTPKAIAAAIEAGKTLEDFAI"
     gene            complement(747..>1184)
                     /locus_tag="ACHKUC_005149"
     CDS             complement(747..>1184)
                     /locus_tag="ACHKUC_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4756 family protein"
                     /protein_id="HFO6621186.1"
                     /translation="VNTVKELKNHIPIEEYRNEYRKLRSDDIPLVKAQKFKSAHTELR
                     RLEKKRESLIEYFIDELNPISSSKANTSARSTGNLDLFNERVLYRKAISEKSDEEIVA
                     LVIKQRTEAAVEFQRSIEHSLEQLSRISSEFEPASQKRRKMSL"
BASE COUNT          315 a          229 c          248 g          392 t
ORIGIN      
        1 caattggcta gcaacaagag atgagataat tctaatattc gggaatgaat catcattatt
       61 ttgtagccat ttggggagat ggattaattt caacttcctg ttcgtagtcg cctctattgt
      121 tgcaggttaa ttactgatgg agcgaaagta aaaccgccaa attagattga cggttttatt
      181 gtgattagat cgcaaagtct tccagcgttt taccagcttc gatggcagca gctatcgctt
      241 taggagtacg gccctgaccc gtccaagtct gctcattgcc attttcatct gtatatttgt
      301 atttagcagg gcgtggagtg cgtgtcgatt tggctttagg ctgggatgaa ctaacagcag
      361 caaggagttc tgctggatca ataccatctt ctaacagttt ctggcggaag gcttccagtt
      421 tagcctgacg ttcttcttgt tctttacgga cagcactttc ttcctcacga cgatcttcaa
      481 cgacagcaga gagcttttcc agcatctctt ccagagttgt cagatctgtt tcacgagcat
      541 gtgcgcgaag tgtacgaata ttgttcagtg ccttgagagc ttcactcata gtatgtcatc
      601 cggtgtttta aaatgggcga ttaataaaaa taatgcttac ggtgctgttg gaaaaatcaa
      661 aaaaaacagt cagtaaataa tatagtaaac attattgcat aagcaatcta atacatcaca
      721 tttcacacga atagcataga gtcgttttaa agagacattt tcctgcgctt ttggcttgct
      781 ggttcgaact ctgacgagat acgagatagt tgctcaagac tatgctcaat tgaacgctgg
      841 aactcaacag cggcttctgt gcgttgtttt atgaccaagg ctactatttc ttcatcactc
      901 ttttctgaaa tagccttacg gtataacacg cgttcgttaa ataaatcgag attgccagtt
      961 gatcttgctg atgtgttagc ttttgaactg ctgatgggat ttaactcatc aataaagtat
     1021 tcaataagag attctctctt tttttccagc ctgcgaagtt cggtatgggc agacttaaat
     1081 ttctgagctt taaccaatgg aatatcatcg gaacgcagtt tacgatattc atttctgtat
     1141 tcttctattg gaatgtgatt ttttagttct ttgactgtat ttac
//
LOCUS       DAZFRG010000514         1170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFRG010000514 DAZFRG010000000
VERSION     DAZFRG010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.517"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1040
                     /locus_tag="ACHKUC_005150"
     CDS             <1..1040
                     /locus_tag="ACHKUC_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621187.1"
                     /translation="MGRDREFCYPGTPENNTTPATFFYDFGTVIISDVNNNSAGTVLP
                     SQNWKIGRYKAYCNSLDDYEVYFSGISGIDPSGASGNPQGADIFIPVTHEISISTHIK
                     LYNRNGTMTDKTVPFENYSTNYPTDRSKADYWSSGTEGYIKIRLDKKIISDVSLNNVL
                     LVSLYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGTSFTVNMPDVWASELSRAG
                     AGAKPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATRDTNGKQSIIQAQDNPDVG
                     IMIMDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVTFLALPVSTTGKVPAAGRY
                     NALAILRVEYQ"
BASE COUNT          366 a          207 c          255 g          342 t
ORIGIN      
        1 aaatgggccg ggatcgtgaa ttttgctatc ctgggactcc agaaaacaat actaccccag
       61 cgaccttttt ttatgatttt ggtacagtta ttatttcaga tgttaacaac aattcagctg
      121 gtactgtttt accatctcaa aactggaaaa taggtcgata taaagcatac tgtaattctc
      181 ttgatgatta tgaagtttat ttttcaggta tttcaggaat agatccttca ggtgcttcag
      241 gtaatccaca gggagcggat atatttattc ctgttacgca tgaaatatct atttcaacac
      301 atataaagct ctacaataga aatgggacga tgacagataa aacagtacca ttcgagaact
      361 atagtactaa ttatcctact gacagaagta aagcagatta ctggtcttca ggaacagaag
      421 gctatattaa aattaggttg gataaaaaaa tcatctctga tgtttctctg aataatgtac
      481 ttttagtttc attgtatgcc agtcagcggc aaacggaaca tgggccagta ccattattta
      541 atgcctggat cagtaacctg gatattcagg ttccgcaagg ctgcacaatt aatgagggta
      601 cgagttttac tgttaatatg ccggatgtgt gggccagtga attgagccgg gctggtgctg
      661 gagcgaagcc tgctggtgtt actcctgtag caacaactat tccgattaat tgtacgaata
      721 aagatacaga tgcggtaatg acgttggtat tcgacggtaa catttccgcc acacgtgata
      781 ccaatgggaa acaaagcatt attcaggcac aggataatcc tgatgttggt attatgatta
      841 tggatagcca gcaaaactcc gtagatttaa atgctctggc aacatcagta ggcgttccgt
      901 tcagattggt ggaaaaccag gctgcttctg cgcaaactgc tgatgttact ttcctggctt
      961 tacctgttag tacaactgga aaagtgcctg cggcaggtcg ttataatgca ctggctatat
     1021 tgcgagtgga atatcagtaa tttttgagct agtacagaat cggtttagcc atcaccgcaa
     1081 aataaaatgc aataataggg atgaacagaa tgtaaaacaa aaaaatattt ttaattctac
     1141 aggggaaaaa cctggtggaa aatgtaggtt
//
LOCUS       DAZFRG010000515         1166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFRG010000515 DAZFRG010000000
VERSION     DAZFRG010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.518"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1166
                     /locus_tag="ACHKUC_005151"
     CDS             <1..>1166
                     /locus_tag="ACHKUC_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6621188.1"
                     /translation="QTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTA
                     VEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTEL
                     LETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLDREVTLP
                     ETGTATVNLINGSGKPASVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFR
                     CVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAA
                     DGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTL
                     TVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTIQF"
BASE COUNT          242 a          350 c          377 g          197 t
ORIGIN      
        1 cagacgctga cgttcgttca ggaccgcccg tcggatgtgg tgtggccgta caccaacagc
       61 gatgtggtgg tggatgataa cggcgtgggg ttccgctaca gcttcagtgc cctgaaggac
      121 cggcacacgg cggtggaggt gaattacacc gacccgcaga acggctggca gacctccacg
      181 gaactggtgg aagacccgga agccatactg cgctacggac gcaacctgct gaagatggac
      241 gcgttcggct gtaccagccg ggggcaggcg caccgtgccg ggctgtgggt gataaagacc
      301 gaactgctgg aaacgcagac ggtggatttc acgctcgggt ctcaggggct gcggcacaca
      361 cccggtgaca tcattgaaat ctgtgataac gactatgccg ggaccatgac cggcggacgt
      421 gtcctgtcca tcgatgccgc cagccgtacc ctgacgctgg accgggaggt gacactgccg
      481 gagaccggca cggccactgt taatctgatt aacggcagcg gtaagccggc gagcgtggcc
      541 atcaccgcac accccgcgcc ggaccggata caggtcagca ccctgccgga tggtgtggag
      601 acatacggtg tgtggggact ctccctgccg tcactgcgcc gtcgcctgtt ccgctgtgtc
      661 tccgtccggg aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa
      721 aaagaagcca tcgtggataa cggtgcccgc tttgagccgc agtcaggctc cctgaacagc
      781 gtcatcccac cggcagtaca gcacctgacg gtggaggtga gcgcagctga cggccagtat
      841 ctggcgcagg ctaaatggga cacgccgcgg gtggtgaagg gcgtgcgctt cagtctgcgc
      901 ctgaccagtg gtaagggaac ggatgccaga ctggtgacca ccgccatcac cgcagacacg
      961 gagcaccgtt tcagtggcct gccactgggg gaatacaccc tgacggtgcg ggcgataaac
     1021 agttatggcc agcagggtga acctgccacc accaccttcc ggattaacgc acctgcaaaa
     1081 cccgccacca ttgagctgac gccggggtat tttcagataa cggcggtccc gcgtcttgcg
     1141 gtgtatgatc cgacgataca gtttga
//
LOCUS       DAZFRG010000516         1161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFRG010000516 DAZFRG010000000
VERSION     DAZFRG010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.519"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..582)
                     /locus_tag="ACHKUC_005152"
     CDS             complement(<1..582)
                     /locus_tag="ACHKUC_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFO6621189.1"
                     /translation="MSLIDLRDEYEERARDIMELLSLASSLEAQTQQLDQQTHKDEIE
                     SNTLRVNILKSSVHMMLYNQVENTARGCIESIYDHLQDNEVNYASLREKFQVNILHRI
                     ISDNESGKSLYQKIGSDISKRIISASLNIRKEFNGNVCKSVLHKITQAYGITVANSPE
                     CRNGVDLDLLKDIRNELAHGSTSFSKKGQIDPLE"
     gene            complement(585..>1161)
                     /locus_tag="ACHKUC_005153"
     CDS             complement(585..>1161)
                     /locus_tag="ACHKUC_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO6621190.1"
                     /translation="SGVKRLEAMEVRHGSDAATSIFYRDVVTPCSTNPLFASLAPLSD
                     RKRSNGDHRELVLRFFAYLNDLENYKGYVAPFIDNYLNVQAERVTQQDVDKFKHDFES
                     MLAFVDAHFPMGFKKTPTSKTTPRARYEAIAVGTALALKANPHLQAPVIPVGDWLFGE
                     EFETIVTADSANNTSQLKNRIFYVKDKLLGM"
BASE COUNT          333 a          229 c          207 g          392 t
ORIGIN      
        1 ttctaaaggg tctatttgac cttttttaga aaagctggta cttccatgtg ccagctcatt
       61 tctgatatct ttaagtaaat ctaagtcaac accatttcta cactctggtg agtttgcaac
      121 agttattccg taagcttgtg ttattttgtg caatacagac ttgcaaacat taccattgaa
      181 ttctttacgt atgtttaatg aggctgaaat gatcctttta gatatatcgg aaccaatttt
      241 ttgataaaga gattttcccg attcattatc tgaaatgata cggtgcaaga tatttacttg
      301 gaatttctcc ctgagtgatg cgtaatttac ttcattatct tgcaaatggt cataaattga
      361 ctcaatgcat cctctggcag tgttttcaac ctgattgtac agcatcatgt gcacggatga
      421 ttttaaaatg ttaaccctaa gggtatttga ttctatttca tctttatgcg tttgctgatc
      481 taactgctga gtctgagcct ctaaagatga cgcaagagaa agcaattcca taatatctct
      541 tgctctttct tcgtattcat ctcttaaatc aatcaaactc atagttacat tcctaataat
      601 ttatctttga cataaaatat tctgtttttg agttggctgg tgttgttggc actatcagca
      661 gtaactatgg tttcaaattc ctccccaaac agccagtctc ctacaggaat aaccggagcc
      721 tgtagatgtg gattggcttt taaggcaaga gcagttccca ctgctatggc ttcgtatcta
      781 gcacgggggg tggttttgct tgttggtgtc tttttaaacc ccatagggaa atgagcatct
      841 acaaaagcaa gcatgctttc aaaatcatgt ttaaatttgt ctacatcttg ttgtgtgacc
      901 ctttcggcct gaacattaag ataattgtca ataaaaggag cgacataacc cttataattt
      961 tctaaatcat ttaagtatgc aaaaaatctc aaaactaact cacgatgatc gccattcgaa
     1021 cgtttccgat ctgataatgg agctagacta gcgaaaagtg gatttgttga gcaaggagtc
     1081 acaacatccc tatagaaaat tgaagtggcc gcatctgaac catgtctaac ctccatcgct
     1141 tccagtctct taacacctga a
//
LOCUS       DAZFRG010000517         1160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFRG010000517 DAZFRG010000000
VERSION     DAZFRG010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.520"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..193
                     /locus_tag="ACHKUC_005154"
     CDS             <1..193
                     /locus_tag="ACHKUC_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HFO6621191.1"
                     /translation="DIRLLAYLFYRSDKNVELRPLLADTLTIHTDDEQLTLLWRLTLP
                     VDSLPQRIVLTTEQRGPHG"
     gene            186..>1160
                     /locus_tag="ACHKUC_005155"
     CDS             186..>1160
                     /locus_tag="ACHKUC_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HFO6621192.1"
                     /translation="MAENHIGCRESKYVAICIAPDVCKVGDAIVPFDSFQDLTHEKIY
                     ISNVRARGNPILTVDCIIAGTRSNAGKGVSSGTSLGSGDCRILTGVDHIKCKGKPVAR
                     NLSLVAMNNDNTVGKLYTQTNPPNQIIPVKESSLWARYLELQRQQQEFELESKKQEAD
                     AMIGLGKAFINGWYMLGALIAKGQMQNDIAQMESNIALARGLGMDTQIMERANETNRE
                     VMETIDPREPLLKPANETQAMAMEVEPWLELMTGVGALVKGLVKFSAKFAIKSVGRMG
                     RSGAKIVLDANGLPIPVPPSVGAAGRVPRVLQTGGNTLEARTARELNEF"
BASE COUNT          338 a          220 c          319 g          283 t
ORIGIN      
        1 agatattaga ctattggcgt atttgtttta ccgctctgat aaaaacgtag aactgcgccc
       61 attgttggct gacacactga ccatccacac agacgatgag caactaacac tactctggcg
      121 cttaacgctc cccgtggatt cacttccaca acgtattgtt ttgacgacgg agcagagggg
      181 acctcatggc tgaaaatcat attggttgtc gtgaatctaa gtacgttgcg atatgtattg
      241 cgcctgatgt ctgcaaggtt ggtgatgcga tagtgccgtt tgacagcttt caggacctga
      301 cacatgagaa aatttatatc agcaatgtca gggcacgggg caatccaata ctgaccgtgg
      361 actgtattat tgcaggtaca cgaagtaatg ccgggaaagg tgtcagttct ggcaccagtc
      421 tgggcagcgg tgactgccgg atattgacag gcgtggatca tataaagtgc aagggaaagc
      481 cggtagccag gaatctttcg ctggttgcca tgaataatga taatacagtt ggtaaactgt
      541 atacccagac aaacccgcca aatcagataa tacctgttaa agaatcatcg ctctgggcca
      601 ggtatctgga actgcaaaga caacagcaag agtttgagct tgagtccaaa aagcaagaag
      661 cagatgcaat gataggatta ggcaaggcat ttattaatgg ctggtatatg ttgggtgcat
      721 tgattgccaa aggtcagatg caaaatgaca ttgcccaaat ggaaagtaat attgctctgg
      781 cgagagggtt gggaatggat acccaaatta tggagcgggc gaatgagacc aacagggagg
      841 taatggaaac tattgatcct agagagccat tgttgaaacc tgccaatgaa acccaagcaa
      901 tggcgatgga ggttgagcca tggctggaac ttatgaccgg ggttggagca ctggtgaaag
      961 gtttggtcaa gttttcagcg aagtttgcaa taaaatcggt tggcagaatg gggaggagcg
     1021 gtgctaaaat tgtgctggat gcaaatggat tgcctattcc tgttcctcca agcgttggcg
     1081 ctgcgggaag agttccgaga gtattacaaa ctggaggtaa tactcttgag gcaagaactg
     1141 ccagggagtt aaatgaattt
//
LOCUS       DAZFRG010000518         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFRG010000518 DAZFRG010000000
VERSION     DAZFRG010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.521"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..215)
                     /locus_tag="ACHKUC_005156"
     CDS             complement(<1..215)
                     /locus_tag="ACHKUC_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6621193.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVR"
     gene            complement(333..528)
                     /locus_tag="ACHKUC_005157"
                     /pseudo
     CDS             complement(333..528)
                     /locus_tag="ACHKUC_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(599..>1159)
                     /locus_tag="ACHKUC_005158"
     CDS             complement(599..>1159)
                     /locus_tag="ACHKUC_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6621194.1"
                     /translation="SSDNNDFRARGRGWLGSLETGLPFSITDNLMLEPQLQYTWQGLS
                     LDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKHSVRELPV
                     NWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGV
                     QAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          265 a          307 c          327 g          260 t
ORIGIN      
        1 cgcacacggg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag gctttccagt
       61 acggtgatgg tgggaatgta cgcatatcgt ttactgcggg aagtatgccg gtcttcaccg
      121 aaaatactgg gaacatagtg catcagttct tcgtgtgtca gcggacggtc acggcgtatc
      181 tggttcgcat aaccaaaacg actggctagt cgcataattt gctccttatc ggtggttacg
      241 atttactggt gtaataaatg aaaaagccac gtctcccgga gaagacgcgg cctgacagat
      301 gaaatgaatg acgtttattg tctgagaagc ccttaactgg cgagctgagt attaagctgt
      361 gttccggcat caccagcgca actgaccttc agcattacgg ataaccagcc gggaatatgt
      421 tccctggtca tcttcagtaa acacattgcg gtaagctgtt ttgacggcaa cagcctgttc
      481 gcgtgagaaa gggccttcgg gcaataaacg agatgtacaa cccggcatat ctgttgctcc
      541 ctgaaagtaa aaagccccgg tcatgatgac cggggcctga aggagagtga cctgattatc
      601 aaaaagtcac attcagcgtg gcctgaccgt tataaccttc agcgctgctg ccgctgacgc
      661 tgtgggcata accggcctga acgcccaggg tgatattttc ccggacacgg gcttccagtc
      721 cggcctgcag gtccagtgac gtgccattcc gggacggtga gaacgtcatg ttactgccgg
      781 cggcggctgt acccatgctc atgtcacccc gggagctgaa agtgcggata acagaaggct
      841 gtacccacca gttcaccggc aattcacgca cactgtgttt ggcactgtcg cgcagggtgt
      901 cacgggatga ggtgccttta ccaaagttca tatcgttgtg gctgcccaga cggaagccgg
      961 cacgcacatg ttgtgcactg ccatgcccga acttcacata accggcgtta tcctggccgt
     1021 catccaggga gagtccctgc caggtgtact gcagttgtgg ctccagcatc agattgtcag
     1081 tgatactgaa gggcagaccg gtttccagtg agcccagcca gccccggccc cgggcgcgga
     1141 agtcgttatt gtccgatga
//
LOCUS       DAZFRG010000519         1155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFRG010000519 DAZFRG010000000
VERSION     DAZFRG010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.522"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1155
                     /locus_tag="ACHKUC_005159"
     CDS             <1..>1155
                     /locus_tag="ACHKUC_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6621195.1"
                     /translation="ATDIGIISCAGFLSYGISKFISGLLSDRSNSKIFLSLGLFLSGL
                     VNLLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYSSKERITWATLWNL
                     SHNVGGALAPVLVGFSFGFFGDSALEHAKAAFIVPGALCMAMSIFIYFLQVDRPISVG
                     LPPIEKWKGEEYAETPEIKMSMSTIIKNHIIKNNKLIYCCIYGSFVYILRYGIVSWAP
                     KFLSDSLDVGGKGMGKLVSMGGFSVFEVGGVAGMLLAGYLSVKVFRNSKPLTNTLFLS
                     LTILLLIAYWYVPAGSDYLYLNYAILIFLGLAIYGPVMFIGLYSMELVPKEAAGAASG
                     LSGTFSYIFGSIVATLGMGLVVDHLGWGATFIILILSAVFAIMFTLMSRE"
BASE COUNT          306 a          188 c          255 g          406 t
ORIGIN      
        1 cagcgacaga tattggaatc atatcatgtg ctggattctt atcttatggt atatcaaagt
       61 ttatatcagg actgttgtct gacaggagta actcaaaaat attcttatct ttgggactat
      121 ttctttccgg tttagtgaac ttattgattg gatatatacc gggaattatc acttcagtca
      181 cgttattctc aacgatgtat ctgttaaatg gttggatcca gggaatggga tatccacctg
      241 gggcaaaaac actggtcttc tggtactcaa gtaaggagag aatcacttgg gccacattgt
      301 ggaatctatc tcataatgta gggggagcac tggcacctgt attagttggg ttcagttttg
      361 gattttttgg cgatagtgca ctggaacatg caaaagcggc ctttattgtt ccaggggcat
      421 tatgtatggc tatgagtatt tttatatatt ttcttcaggt tgacagacct atttctgtag
      481 gacttcctcc aattgaaaaa tggaaaggtg aagagtacgc agagacacct gaaataaaaa
      541 tgtctatgtc tacaataatt aaaaatcata ttataaaaaa taataaactg atttattgct
      601 gtatttatgg atcatttgtt tatattctcc gatatggtat tgtatcgtgg gctccaaaat
      661 ttctaagcga tagcctggat gttggaggaa aagggatggg gaaactagtc tctatgggag
      721 gattctctgt ttttgaagtt ggaggcgttg ctggtatgtt actggcaggc tacctgtcgg
      781 ttaaggtttt cagaaactcg aaaccattaa caaatacctt atttttatct cttacaattt
      841 tgctactcat tgcatactgg tatgtacctg caggaagtga ttatctttat cttaactatg
      901 caattctcat ttttctgggg ttagctatct atggcccggt tatgtttatc ggtctttatt
      961 caatggagct tgtcccaaaa gaagctgcag gtgctgcgtc aggtctgtct ggtacctttt
     1021 catatatctt tggttctata gtcgctacat taggaatggg acttgttgtt gatcatctgg
     1081 gatggggtgc aacatttatt attctgatac tttccgctgt tttcgctatc atgtttacat
     1141 taatgagtcg agaac
//
LOCUS       DAZFRG010000520         1155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFRG010000520 DAZFRG010000000
VERSION     DAZFRG010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.523"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..>1155)
                     /gene="ompC"
                     /locus_tag="ACHKUC_005160"
     CDS             complement(89..>1155)
                     /gene="ompC"
                     /locus_tag="ACHKUC_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6621196.1"
                     /translation="AISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSND
                     ADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFG
                     DYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLV
                     DGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQVIYGNN
                     SLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQF
                     DFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDF
                     TKASGVATDDIVAVGLVYQF"
BASE COUNT          318 a          264 c          253 g          320 t
ORIGIN      
        1 gatagtgctt ctaaggaaag tggcttgagg gcgccactta tatgttgggg aggcaaagcc
       61 tcccacaaca tatctttgag taatcagatt agaactggta aaccaatcct acagcaacga
      121 tatcatcggt agcaacgcca gatgctttcg tgaaatcgct cttatcaatc aggttgattt
      181 tgtaatcaac aaaagtggac atatttttgt tgaagtaata ggttgcacct acatcaatat
      241 attcaaccag gtcctgatca ccccacgcac ccaagtcttt tcctttagat tgcaggtaag
      301 caacggacgg acgcagaccg aagtcgaact gatattgtgc aactacttcg aagttttgtg
      361 ctttgttggc aatatggtta ttaccaaaaa cagtcatgtt ctgggtttca gaataggtgg
      421 tagccagata gatgttgttc gcatcatatt tcagaccagc tgcccatact tcagcatttt
      481 gaccagatgc attcaggctg ttgttaccgt agataacctg atcattagtg cggtcagatt
      541 tagcataggt tgcacctaca ccgaatcctt catactcata agtagtggag aaaccgaaac
      601 catcaccgtt agcttcagtt acgtcagtgc ggtcattttt accctgatac tgagcagcaa
      661 agttcagacc atcaaccaga ccaaagaagt cgttgttacg ataggttgca acacctgtgg
      721 tgcgaccagt catgaacaca tctgtttggg tccaggtatc gccaccgaat tctggcagaa
      781 cgtcggtcca tgcaccgata tcgtatgcta caccgtagtt acggccataa tcgatggagc
      841 cgtagtcacc gaatttcagg ccagcgaagg caagacgggt tttatctttg gaagaacctt
      901 gagattcagc acggttgcct ttgaattcat attcccactg accgaaacca gtcagttgat
      961 cgttgatttg agtttcgcct ttgaagccca gacgaacata agttgtgtcg ccatcatctg
     1021 catcgttaga agagaagtag tgcttagcat taacttttcc gtacagatcc agcttgttac
     1081 tgtctttatt gtaaatctct gctgcctgag cagacatcgc catcagtact gatgcagcta
     1141 cagcagaaat tgcca
//
LOCUS       DAZFRG010000521         1154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFRG010000521 DAZFRG010000000
VERSION     DAZFRG010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.524"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(201..398)
                     /locus_tag="ACHKUC_005161"
     CDS             complement(201..398)
                     /locus_tag="ACHKUC_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6621197.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            652..>1154
                     /locus_tag="ACHKUC_005162"
     CDS             652..>1154
                     /locus_tag="ACHKUC_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO6621198.1"
                     /translation="MTTLKELKEELAQIQDENAKNRKKAEIAALVTSADNEIRLAQRN
                     IGYNVREWTVELIVQKYGNNLETDKNELFIPDYQRDYKWDIKTASRFIESILLDFPIP
                     YLYISDVFNEDPELDGRVEIIDGSQRIRSIYYFWNNQFELKDLKELKSLEGFKFSDLL
                     ASRQRRF"
BASE COUNT          342 a          257 c          234 g          321 t
ORIGIN      
        1 cagtcggcgt tgattaactc acaactggat atttttacag tattaagcat ggatcattaa
       61 gcctgtctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
      121 tgaacccaac gcatgagcag atggctggtg ggtgccccta acacccacca gccgcccatt
      181 taccacaaat aaaaaagcct tcactgcgga aggcgtctgt aacaaccgaa ctgatagtct
      241 gccagacccg ccataaccag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
      301 gtattctgcg ctatctcccc gactgtcgcc ggttcggtaa cgcttaattc attaaacact
      361 actctggcgg tttctgtcat atcctgctgt tttagcatgt ctttttccct tttccggtta
      421 acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagaca ggtattcgca
      481 accaccagca cgtttaacgt actgcgccgt ttttcggaca caaaaaaccg cctggcggcg
      541 ggtttaagct gtgtggcgta gtaaccactc ttaacacgat ataatatttt ttgcgtacgc
      601 gttatagttt tcttacaatc tatttccaac tccaattaaa ggaacagtga tatgaccaca
      661 ctcaaagaac tcaaagaaga gcttgctcag attcaagatg aaaacgcgaa aaatagaaaa
      721 aaagctgaaa ttgcagcctt ggtcacttct gccgacaacg agattaggct tgctcagaga
      781 aatattggat acaacgtacg tgaatggacc gttgaattaa ttgtacaaaa gtatggcaat
      841 aatcttgaaa ctgacaaaaa cgaacttttt attcctgatt atcaacgcga ttacaaatgg
      901 gatatcaaaa cagcctctcg attcatcgaa agtatattat tagattttcc aatcccgtac
      961 ctctacatct ccgacgtatt taatgaagat cctgagttgg atggtcgtgt agaaattatt
     1021 gacggttcac aaaggattcg ctcaatttac tacttttgga acaatcagtt tgaattgaaa
     1081 gacttaaaag aactcaaaag tttagagggc ttcaaatttt cagatctttt ggctagccgc
     1141 cagaggcgat tttt
//
LOCUS       DAZFRG010000522         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFRG010000522 DAZFRG010000000
VERSION     DAZFRG010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.525"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..755
                     /gene="sdiA"
                     /locus_tag="ACHKUC_005163"
     CDS             33..755
                     /gene="sdiA"
                     /locus_tag="ACHKUC_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO6621199.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(823..1047)
                     /gene="yecF"
                     /locus_tag="ACHKUC_005164"
     CDS             complement(823..1047)
                     /gene="yecF"
                     /locus_tag="ACHKUC_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO6621200.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1034..>1153)
                     /gene="yecU"
                     /locus_tag="ACHKUC_005165"
     CDS             complement(1034..>1153)
                     /gene="yecU"
                     /locus_tag="ACHKUC_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO6621201.1"
                     /translation="TLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          295 a          249 c          281 g          328 t
ORIGIN      
        1 gatactcact ctcaggggcg ttgcggttta ctatgcagga tacggatttt ttcagctggc
       61 ttcgcacgat gttgttgcgt tttcagagga tggaggccgc agaagaggtc taccatgaaa
      121 tagagcttca ggctcagcag ctggagtacg attactattc gttatgtgtt cgccacccgg
      181 taccattcac gcgacctaaa gtggctttct acaccaatta ccctgagtcg tgggttagtt
      241 attatcaggc aaaaaatttt ctcgcaattg atccggtgct gaaccctgaa aactttagtc
      301 agggccattt aatgtggaat gatgacttat tcagcgaagc acagccgtta tgggaagctg
      361 cgcgcgcaca tggtttacgc cgcggtgtca ctcagtattt aatgctgcca aatcgggcgt
      421 tgggcttttt gtccttttcc cgttgcagca cgcgcgaaat acccattctt agtgatgaac
      481 tgcaattaaa aatgcagcta ctggtgcgcg aaagtctgat ggctctgatg cgtttaaatg
      541 atgaaatagt gatgacgcca gagatgaatt tcagcaagcg cgaaaaagaa attctgaaat
      601 ggacggcgga aggtaagaca tcagcagaga tagcgatgat tttgtcaatc tctgagaata
      661 cggtcaattt ccatcagaaa aacatgcaga aaaaaatcaa tgcaccgaat aagacgcagg
      721 ttgcttgtta cgcggccgct actggcttaa tttgatctct tttctgttct gcgtgccaga
      781 tgcaaaaacc ggctgaaagg cacgctctca gccggtttta tattactgac ggtaggcttg
      841 tttaatttgc ttaaccgttt tggaaaatac tgcagcctgg gtttcgtctt cgatcagcgc
      901 aagctgtttt tccatcttta acatcacgcg gcccgcgtca gcttgtccca tcgcctgcag
      961 catcagcgtc agcatcgctt tcaggcagga gacttcattt gccagttctt gattattctc
     1021 ggcagtagaa aaatcaggcg tactcattta ttttcctcgt catgttgcaa tgaaaatttg
     1081 cggtgaaaaa tgttaaggcg gcggagtata ccataagctt tgctaaaaat agcagtggtt
     1141 gttttttgag cgt
//
LOCUS       DAZFRG010000523         1142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFRG010000523 DAZFRG010000000
VERSION     DAZFRG010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.526"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..>1142
                     /locus_tag="ACHKUC_005166"
     CDS             122..>1142
                     /locus_tag="ACHKUC_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6621202.1"
                     /translation="MNDRLRFEVNDNQGWFVFPDAWFGPLLDEFDELLDAYDADEISE
                     TSYINKLRRLSRQEPDFIDVHAHLAYVFLEQNSPRKALNAALKGMVVANRLIPESFSG
                     EIIWMNPQNRPYLRALYATILANVHLQRHRHAVMLIEKILAYNPEDNQGARWLLGSEL
                     LRAGDHERALRVLKSHTGEFSPCWYELGLLHFLNGEHVKAATAFRHGFVTNTYIAEML
                     CGNRHPFPLAVWHNFSCGPDTAEDYYVTYSPLWGEYPEALLFVNWLCNHSAVLHERAE
                     IMKCAEMLMQEDDVEICERILRQQEKLLERIDERSSEEIVQKCRTWRGEEVWPWILPF
                     TAGGIN"
BASE COUNT          306 a          268 c          292 g          276 t
ORIGIN      
        1 ggcaggtatg cactggcttc gtaagaaatg ttcattcggg ggccaggaca gagcctgata
       61 cacagcatct caacctttat gctcccggac catatcacca caggttttac aggctcagaa
      121 aatgaatgac agattacgct ttgaagtgaa cgataaccag gggtggttcg tattcccgga
      181 cgcctggttc ggcccgttgc tggatgagtt tgacgaactg cttgatgcat acgacgcgga
      241 tgaaatcagc gaaacaagtt atatcaacaa actcaggcgt ctttcccgac aggagcctga
      301 ctttattgat gtccacgctc acctggcata cgtatttctg gaacaaaact ctccgcgtaa
      361 ggcactgaac gctgcgctta aaggaatggt ggttgctaat cgccttatac ctgaaagttt
      421 cagcggtgaa atcatctgga tgaatccgca aaaccggcct tatctgcgcg ccctttatgc
      481 aacaattctc gcaaatgtgc acttacagcg tcaccggcat gctgtcatgc tcattgaaaa
      541 aatactggcg tacaaccccg aagataatca gggagcacgc tggttgctgg ggtcggaact
      601 gttacgtgct ggcgaccatg agcgcgcact ccgtgtactg aaatcgcata ccggtgaatt
      661 ctcaccctgc tggtatgaac tcggccttct gcattttctg aacggggaac atgttaaggc
      721 tgccacagct tttcgccatg gctttgtcac aaacacttac atcgcggaaa tgctgtgcgg
      781 aaaccggcat ccatttccac ttgctgtgtg gcataacttt tcatgcggtc cggatactgc
      841 tgaagattat tatgtaacat actccccatt atggggagaa tacccggaag cactgctgtt
      901 tgtaaactgg ctctgtaacc attccgctgt gctgcatgaa cgggcagaaa tcatgaaatg
      961 cgccgaaatg ctgatgcagg aagatgatgt cgaaatatgc gagaggattt taagacagca
     1021 ggaaaagttg ctggaaagaa tcgacgaacg gagttctgaa gagattgtac agaaatgcag
     1081 aacatggcgg ggagaagaag tctggccctg gatattgcca tttacagccg ggggaataaa
     1141 tc
//
LOCUS       DAZFRG010000524         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFRG010000524 DAZFRG010000000
VERSION     DAZFRG010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.527"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1140
                     /locus_tag="ACHKUC_005167"
     CDS             <1..>1140
                     /locus_tag="ACHKUC_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO6621203.1"
                     /translation="LSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQ
                     LQPQWVDIPTLVQNTCHSFAAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLS
                     NALKFTTEGAVKITTSLGHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQ
                     QTGSGLGLMICKELIKNMQGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPIT
                     LPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNM
                     PNMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHL
                     SQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKD"
BASE COUNT          351 a          252 c          244 g          293 t
ORIGIN      
        1 ctgtcgggtt ctggtcttag caaggaacaa cgggtggagg cgatttcact tgcctacgcc
       61 accggacaat cactcctcgg cttaattggt gaaatccttg atgtcgacaa aattgaatcg
      121 ggtaactatc aacttcaacc acaatgggtc gatatcccta ctttagtcca gaacacttgt
      181 cactctttcg ctgcgattgc tgcaagcaaa tcgatcgcat taagttgcag cagtacgctt
      241 cctgaacgct acctggttaa gatcgaccct caggcattta agcaggtctt atctaattta
      301 ctgagtaatg ctctcaaatt tactaccgag ggggcagtaa aaattacgac ctccctgggt
      361 cacattgatg acaaccacgc tgttatcaaa atgacgatta tggattctgg aagtggatta
      421 tcgcaggaag aacaacaaca actgtttaaa cgctatagcc aaacaagtgc aggtcgtcag
      481 caaacaggtt ctggtttagg cttaatgatc tgcaaagaat taattaaaaa catgcagggc
      541 gatttgtcat tagaaagtca tccaggcata ggaacgatat ttacgatcac aatcccggta
      601 gaaattaccc aacaagtggc ggctgtcgag gcaaaagcag aacaacctat aacactacct
      661 gaaaagttga gcatattaat cgcggatgat catccgacca ataggctatt actcaaacgc
      721 cagctaaatc tattaggata tgatgttgat gaagccactg atggtgtgca agcgctacac
      781 aaagtcagta tgcaacatta tgatctgctt attactgacg ttaatatgcc gaatatggat
      841 ggttttgagt tgactcgcaa actccgtgag caaaattctt ccttacccat ctgggggctt
      901 acagccaacg cacaggctaa cgaacgtgaa aaagggttaa attgcggcat gaacttatgt
      961 ttgttcaaac cgttgactct ggatgtactg aaaacacatt taagtcagtt acaccaggtt
     1021 gcgcatattg cacctcagta tcgccacctt gatatcgagg ccctgaagaa taatacggcg
     1081 aatgatctac aactgatgca ggagattctc atgactttcc agcatgaaac gcataaagat
//
LOCUS       DAZFRG010000525         1136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFRG010000525 DAZFRG010000000
VERSION     DAZFRG010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.528"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1062
                     /gene="ompC"
                     /locus_tag="ACHKUC_005168"
     CDS             <1..1062
                     /gene="ompC"
                     /locus_tag="ACHKUC_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6621204.1"
                     /translation="ISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDA
                     DDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGD
                     YGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVD
                     GLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQVAYGKSK
                     FNASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEAVAQYQFD
                     FGLRPSVAYLQSKGKDLGVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINLIDDSKFT
                     KTAGIDTDDIVAVGLVYQF"
BASE COUNT          321 a          244 c          258 g          313 t
ORIGIN      
        1 atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc tgaaatttat
       61 aataaagaca gtaacaagct ggatctatac gggaaagtta atgccaagca ctacttctcc
      121 tctaatgatg cagatgatgg tgatactact tatgcccgtc ttggcttcaa aggtgaaacc
      181 caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa aggcaaccgt
      241 gctgaatctc aaggttcttc caaagacaaa acccgtcttg catttgcagg cctgaaattt
      301 ggtgattacg gctcaatcga ttacggccgt aactacggtg tagcatacga catcggtgcg
      361 tggactgacg tcctgccaga attcggtggt gatacctgga cccaaacaga tgtgttcatg
      421 actggtcgca ctactggtgt tgcaacttat cgtaacaacg acttctttgg tctggtcgat
      481 ggcctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt aactgaagcc
      541 aatggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg cgtgggtgca
      601 acctatgcta aatcagatcg cactgacggt caggtcgcct atggtaagag caaattcaat
      661 gcctccggca aaaatgcgga agtatgggct gcaggcctga aatatgatgc gaacaatatc
      721 tatctggcta ccacatattc tgaaactcag aatatgaccg tttttggtaa taaccatatt
      781 gcaaacaaag cacaaaactt tgaagcagta gcacaatatc agtttgactt cggtctgcga
      841 ccatctgttg cttaccttca gtcaaaaggt aaagaccttg gtgttcatgg tgaccgagac
      901 ttagtcaagt atgtcgatgt cggtgctact tactacttta ataaaaacat gtccactttt
      961 gttgattaca aaatcaactt aattgacgat agtaagttta ccaaaacagc tggtattgat
     1021 accgacgaca tcgtcgctgt aggtctggtt tatcagttct aatctgactt acgaaaaaga
     1081 tatgttgcgg gaggctttgc ctccgcaaca tataagtgga gccctcaagc cacttc
//
LOCUS       DAZFRG010000526         1134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFRG010000526 DAZFRG010000000
VERSION     DAZFRG010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.529"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1134
                     /locus_tag="ACHKUC_005169"
     CDS             <1..>1134
                     /locus_tag="ACHKUC_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6621205.1"
                     /translation="TVSMGNGGVLLADSGAAVSGTRSDGKAFSIGGGQADALMLEKGS
                     SFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGATLTLSGKTVNNDTLTIREGDA
                     LLQGGALTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTA
                     LKLTGSTVLNGAIDPTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFV
                     PTTLQVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLA
                     TTGKGIQVVEAINGATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSENAYRAEVP
                     LYASMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLG"
BASE COUNT          273 a          327 c          333 g          201 t
ORIGIN      
        1 ccaccgtatc catggggaat ggcggtgtac tgctggccga ttccggtgcc gctgtcagtg
       61 gtacccggag cgacggaaag gcattcagta tcggaggcgg tcaggcggat gccctgatgc
      121 tggaaaaagg cagttcattc acgctgaacg ccggtgatac ggccacggat accacggtaa
      181 atggcggact gttcaccgcc aggggcggca cactggcggg caccaccacg ctgaataacg
      241 gcgccacact taccctttcc gggaagacgg tgaacaacga taccctgacc atccgtgaag
      301 gcgatgcact cctgcaggga ggcgctctta ccggtaacgg cagcgtggaa aaatcaggaa
      361 gtggcacact cactgtcagc aacaccacac tcacccagaa agccgtcaac ctgaatgaag
      421 gcacgctgac gctgaacgac agtaccgtca ccacggatgt cattgctcag cgcggtacag
      481 ccctgaagct gaccggcagc acagtgctga acggtgccat tgaccccacg aatgtcaccc
      541 tgacatccgg tgccacctgg aatatccccg ataacgccac ggttcagtcg gtggtggatg
      601 acctcagcca tgccggacag attcatttca cctccgcccg cacagggaag ttcgttccga
      661 caactctgca ggtgaaaaac ctgaacgggc agaatggcac catcagcctg cgtgtacgcc
      721 cggatatggc gcagaacaat gctgacagac tggtcattga cggtggcagg gcaaccggaa
      781 aaaccatcct gaatctggtg aacgccggca acagtggtac ggggctggcg accaccggta
      841 agggtattca ggtggttgag gccattaacg gtgccaccac ggaggaaggg gcttttgtcc
      901 aggggaatat gttgcaggcc ggggccttta actacaccct caaccgggac agtgatgaga
      961 gctggtatct gcgcagtgaa aatgcttatc gtgctgaagt ccccctgtat gcctccatgc
     1021 tgacacaggc aatggactat gaccggattc tggcaggctc ccgcagccat cagaccggtg
     1081 taaacggtga aaataacagc gtccgtctca gcattcaggg cggccatctc ggtc
//
LOCUS       DAZFRG010000527         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFRG010000527 DAZFRG010000000
VERSION     DAZFRG010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.530"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..1095
                     /locus_tag="ACHKUC_005170"
     CDS             43..1095
                     /locus_tag="ACHKUC_005170"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO6621206.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTSNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT          294 a          253 c          283 g          302 t
ORIGIN      
        1 ccatatcgca ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc
       61 gtgatgatgc gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tctacccgaa
      121 gatcaaacgc agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa
      181 attgacggta ttggcggcgg taatccgctg accagtaaag tcgccattat tagccgctcc
      241 agcgatccgc gtgcagatgt cgattatctg tttgctcaag ttatcgtcca tgagcaacgt
      301 gtcgatacca cgcctaactg cggcaatatg ctgtctggcg ttggggcatt cgccattgaa
      361 aatggtttga ttgcagcgac ttcgccagtt acccgcgtac gtatccgcaa cgtcaatacg
      421 ggtacattta tcgaagctga tgtgcaaacg tcaaatggtg ttgtcgagta cgagggtagc
      481 gccagaattg acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc
      541 gccggaacga aaaccggaaa agtcttcccg acggataatc agattgatta ttttgacgat
      601 gtcccggtga cctgtatcga tatggcgatg ccagttgtca ttattcctgc tgaatatctg
      661 ggaaaaacag gctatgaatt accggcagag ctggatgccg acaaagcatt attagcccgc
      721 attgaatcta tccgtctaca agcgggtaaa gcaatgggct taggcgatgt cagtaatatg
      781 gttatcccta aacctgtact tatttctcca gcgcagaaag gcggggcaat taatgtgcgt
      841 tattttatgc cgcattcttg ccatcgcgct ttggcgataa ccggtgctat tgctatttcc
      901 agtagttgtg cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatacggc
      961 aatatcaata ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag
     1021 gatgccacga cgttacgcgc atctgttatt cggacgacaa gaaaaatatt ttccggtgaa
     1081 gtttatcttc cctgaaaaat tttgttgtcg tgataatgac aatcaataaa gg
//
LOCUS       DAZFRG010000528         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFRG010000528 DAZFRG010000000
VERSION     DAZFRG010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.531"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..1058)
                     /gene="wzzB"
                     /locus_tag="ACHKUC_005171"
     CDS             complement(81..1058)
                     /gene="wzzB"
                     /locus_tag="ACHKUC_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO6621207.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSSYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT          282 a          283 c          241 g          323 t
ORIGIN      
        1 cctagctggg ggttttctgt gcacaaaaaa acgggcatgg tgtcaccacc ctgcccgttt
       61 ctcttaaatg cacaataata ttatttcgcg ttgtaattac gcagcgcgtt acgcccaagc
      121 acaatccccg cgccaaccat gccgcccagc agcaccgcca gaatcaaggt aattgccttt
      181 ttcgggctat cgcgacgaat aggtaacgtc ggtttcatta catagcggta agcatgaata
      241 tcaagtttat caacgtcaag attgtcgata tccagcaggt tttgacgagt ctgatagtag
      301 cttgatgaga acaccaacgg acgggtcgcc tcgtgcttaa tcatcgactc cagcgcttcg
      361 ctccccaaaa ggaacatagt atcctgggtt acgtcctgcg tctgttgaat ctgcggcttt
      421 gtcacctgcg cctgattcgc atactgcaac gcttcctgaa tctgacggat acgcagatct
      481 ttctgctctt gagcgaccac ttcctgcgtc ttcagagaat cctgcaagtt tttcatttgc
      541 agcgcgatgt tgtctttcag gtcttgttcc agttcttcat ttacctggtc gtcaacttgc
      601 tggatatact tcgccagctg tttctgcgca ccttcaggtg tttcaccaac ataagaaacc
      661 gctaaaggta atgactgatt tttaacggta ggctcaatgg tcagtttttc aggttcttcc
      721 tgattatcca gcgtttctgc taatgctgaa aatgcagtgc tgtaacgtcc gataagtgaa
      781 gcctgaatgt cggagacttt cggtactgcc ggaccgtaaa tcacattcat cgcgttggta
      841 taaccagcga tttgccccac atcaggttga gtaacgatag ctgttgaagt ccacttttct
      901 ttggcaacgg ccagataccc tatagccagg gcaatagcca caatgacaga aatgatgata
      961 gtcatcttgc cacgccatag ttggaccagt aaatcaatca aatcaatctg ttccgggtca
     1021 tggttttgcc cagaaacatt attattttct actctcatca ttaccctaac ggacaaaata
     1081 attttaagtc gccatagttt agcgataatc gccatgaatg ctataggat
//
LOCUS       DAZFRG010000529         1126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFRG010000529 DAZFRG010000000
VERSION     DAZFRG010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.532"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHKUC_005172"
     CDS             complement(<1..186)
                     /locus_tag="ACHKUC_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621208.1"
                     /translation="MDDNNQTSGQPKPEPEECVKEQKITDHFKIMIDKARKAQKLVLI
                     KRADDLLRWGAQEEYDFS"
     gene            complement(176..>1126)
                     /locus_tag="ACHKUC_005173"
     CDS             complement(176..>1126)
                     /locus_tag="ACHKUC_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6621209.1"
                     /translation="EDATGGEQVYIHAQKNMDTEVLNNRTTDVKADHTETIGNDQKIT
                     VVKGQTVQVGTRKEGGHDQSITVANDRCITVRNDQTLQVTNDRTVSVSNDDGLYVRND
                     RKVTVEGKQEHKTTGNHVSLVEGKHSLVVKGDLARKVSGALGIKVDGDIVLESSSRIS
                     LKVGGSFVVIHSGGVDIVGPKISLNSGGSPGTPVPALQPAVLKTLGDEKSGDGSDSGE
                     ENEDSGGNCVTGSGGDDRGDDEDEPEKYTLQFHFTDDDGIPYSETRYIAFFEDGTQTR
                     GETDEEGYTERFFVSSKHEIKVKLLFANDDFLSMEGHYGR"
BASE COUNT          212 a          374 c          206 g          334 t
ORIGIN      
        1 actgaaatca tattcctcct gtgctcccca tctcaaaaga tcatctgctc tttttatcaa
       61 aaccaacttt tgtgccttac gagccttatc aatcattatt ttaaaatgat ctgttatttt
      121 ttgctctttc acacattctt ctggctctgg ctttggttgt ccagaagttt gattattatc
      181 gtccatagtg tccttccatt gagagaaaat catcattcgc aaatagcaac ttcactttaa
      241 tttcatgttt gctagataca aaaaaacgct ctgtataccc ctcttcatcc gtttctccgc
      301 gggtctgcgt gccatcctca aagaaggcaa tgtagcgggt ttctgaatac ggtatcccgt
      361 catcgtctgt aaaatggaac tggagagtat atttttcagg ttcatcctca tcgtcacctc
      421 gatcatcacc accacttccg gtcacgcagt tccctccaga gtcttcattt tcttctccgg
      481 aatcactgcc atcaccactc ttctcatccc ccagtgtctt cagcaccgcc ggttgcagcg
      541 ccggcaccgg cgtccccgga ctaccgccgc tattgagact gattttcggc ccgacgatgt
      601 ccacgccccc tgaatggatg accacaaacg agccacccac cttcaggctt atccggctgc
      661 tgctctccag cacgatatcc ccgtcgacct ttatccccag cgcgccactc accttcctcg
      721 ccaggtcccc cttcaccacc aggctgtgct ttccctccac caggctgaca tggttccccg
      781 tcgtcttatg ctcctgcttc ccttccaccg tcactttacg gtcatttctg acgtacaggc
      841 cgtcatcatt actcacgctc accgtccggt cattcgtcac ctgcagcgtc tggtcattac
      901 gtaccgtgat gcaccggtcg ttcgccaccg tgatactctg gtcgtggccc ccttcttttc
      961 tggtcccgac ctgaaccgtc tgccccttca ccaccgttat cttctggtcg ttaccaatgg
     1021 tctccgtgtg gtcagccttc acatccgtcg tccgattatt cagcacctcc gtatccatgt
     1081 tcttctgcgc gtggatatag acctgttcac cgcccgtggc gtcttc
//
LOCUS       DAZFRG010000530         1124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFRG010000530 DAZFRG010000000
VERSION     DAZFRG010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.533"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..368)
                     /locus_tag="ACHKUC_005174"
     CDS             complement(<1..368)
                     /locus_tag="ACHKUC_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFO6621210.1"
                     /translation="MYPTDPRQLNTERQIYLDKQFFVDVFSIPACVRNTNGDFIGYNE
                     KFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLPSSMNKIQSVAIGDKLWLVQF
                     IPLIYGKVVNVLWLFFCKNS"
     gene            complement(562..945)
                     /gene="traM"
                     /locus_tag="ACHKUC_005175"
     CDS             complement(562..945)
                     /gene="traM"
                     /locus_tag="ACHKUC_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO6621211.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
BASE COUNT          361 a          237 c          173 g          353 t
ORIGIN      
        1 ttagaatttt tgcagaaaaa aagccataaa acattaacca ccttcccata tattaatggt
       61 ataaattgta ccagccagag tttatcacct atagccacgc tctgtatttt attcatggac
      121 gaaggcaaag acatagcatc cagttcttct cttaagaacg atgtagctac ttgtacagga
      181 agggagtaaa accactcttt gatatcaaga ctacctataa attctttaga gaatttttca
      241 ttgtagccga taaaatctcc attagtattt cgaacacaag cagggattga aaaaacatcg
      301 acaaaaaact gtttatccaa atatatttga cgttcagtat taagttgtct aggatccgtc
      361 ggatacatag gaacctcctc tcaaaggatt ctatggacag tcgatgcggg gaggtcgctc
      421 tccctgcatc ggcgattttt aatatctgtt ttaacgtggc attaaccaca taattaatat
      481 accctttatg tggttaattg tcatcaaatt gaaccagatc aaaatcctgt tatttgaagt
      541 aaatcaactt ttaattcttt tttattcctc atcatttttt ggaaaaaatc gttccatctc
      601 agatgacacc ttctcacgaa tatcttcaac catattggca tattcaaact ttggattccc
      661 ggagacatgg ggacttagag actcaatacc caaaattttc gctactgatg actgtgtttt
      721 tacaacacat tcaagaagaa gtttattaaa ctctgtctga ttaaacgcag actctttacg
      781 ctccatctga gcctcatata cacgaagccc cagttcaaga agcattgaag ctgttcccga
      841 aacacttata tctttatctc ttgccccctc ctgacgacgc tgttcaacta tcgcatttac
      901 tttatcatag acatcattac tgatatacaa aattactctg gccataagat aaaacctttc
      961 attattaagc aatgaacttt tcactataaa tatgcatata gtgtttacaa gtaagaaaga
     1021 cactcctagc agcgcctcta ggatcatcct ataaaaaaat gcgatccggc gctaggggcg
     1081 tccctaatat atatcaatgt ttttcatgaa aattgtcagt actg
//
LOCUS       DAZFRG010000531         1117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFRG010000531 DAZFRG010000000
VERSION     DAZFRG010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.534"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..576)
                     /locus_tag="ACHKUC_005176"
     CDS             complement(<1..576)
                     /locus_tag="ACHKUC_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6621212.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPES"
BASE COUNT          372 a          241 c          166 g          338 t
ORIGIN      
        1 actttctgga gcactttttc tgatccaaag aactagtaaa actggtaaca ggcctataaa
       61 aaaagagttt ctccatccat atacttcagc aaactgaggg attatttgtg ccgcaataat
      121 atttccaaca gaaaaaccac ttaccaaaaa agcactagct ttagattgaa gatttttagg
      181 ccaactttct accgcataag ttgaagcaca tgcatattca ccagacatcc ctaagccaac
      241 aataaaacgg caaactgcga gcatatataa gtttgtagca ataccgctaa ggcctgttcc
      301 gactgagtaa atgaaaattg cccacatcat cattggctta cgaccatatt tatcagccat
      361 ggcaccaaaa aaaccacctc caataggtct ggctatgaag gccactgtcc ctattaaagt
      421 agcctgaata tccgtaatgc caagatctgc ttttataata tgaagaatgt aaaatatcat
      481 cataaaatca aagccatcaa atacatatcc aagccatgcg gaaaaaagag ctttccgttg
      541 tggtggatta acttgtttat accatgctgt tgccatattt gtttcctgtg ttattctaat
      601 atagccctta aagtaaatat tagggagcct caacaatatc cagaaataat tatattgtct
      661 tcaaatacgc cagtactatt gattgttatc aatacctaat cagtacaatc ttttcctcca
      721 tttcttctta acatatttca ctctatgaaa gataaaactg atacccacct acagttttgg
      781 gtcaccaaca aaaaacaatg ttcgccagat attccaccac tggcacagtt caaccctgct
      841 tcaaaaattg acttattcat tgtgctcatt ttcgtagtcc gttcaggttg aataaaactc
      901 ccttcgtcaa atgaggaaag actgagaggt tattacttta gcttacgtta tagctgtttt
      961 cctttgcagt atttctttgc ttttctgtat acctttatac ctgttatacc agatcaaaaa
     1021 acaagcaatc cacataacaa aacgcgtttt gttactgatg tcacaaattg agcatatttt
     1081 tgtagctgat agtttgttac aaacaccaga accaaga
//
LOCUS       DAZFRG010000532         1091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFRG010000532 DAZFRG010000000
VERSION     DAZFRG010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.535"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            163..498
                     /locus_tag="ACHKUC_005177"
     CDS             163..498
                     /locus_tag="ACHKUC_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO6621213.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            658..>1091
                     /gene="yjdN"
                     /locus_tag="ACHKUC_005178"
     CDS             658..>1091
                     /gene="yjdN"
                     /locus_tag="ACHKUC_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO6621214.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQP"
BASE COUNT          295 a          276 c          256 g          264 t
ORIGIN      
        1 acgattattt tctttttgcg tttcctttca aatgaaaacg atcgtcatct aaaatcagca
       61 gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg caatctgccg
      121 ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt accacactgc
      181 ccaaaatgca actccgaata cacctacgaa gataacggca tgtacatctg cccggaatgt
      241 gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat cgttaaagat
      301 gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga tctgaaagtg
      361 aaaggtagct cttcgatgct gaaaattggc accaaagtaa aaaacatccg cctggttgaa
      421 ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact gaagtctgag
      481 tttgtgaaaa agaactgatt gtattgtgat cggtaggccg gataaggcgc tcgcgccgca
      541 tccggcaacg gtggcgactg cctgatgcga cgctgaccgc gtcttatcag gcttccctct
      601 catctgtata aatttcgaac tacacttaac gggcttctct taactgaggt caccatcatg
      661 ccgttaagtc cctacctctc ttttgccggt aactgttccg acgcgattgc ctattatcaa
      721 cgtacgttgg gcgcggaact gctctataaa atcagcttcg gcgaaatgcc aaaatcaacg
      781 caggacagcg ccgagaactg cccttccgga atgcaatttc ccgataccgc catcgcccac
      841 gccaatgtgc gcattgcagg aagcgacatc atgatgagcg atgctatacc gtcaggaaaa
      901 gccagctact ccggctttac gctggtgctc gattctcaac aggtggaaga aggaaaacgc
      961 tggtttgata accttgccgc caacggaaaa atcgaaatgg cctggcagga aacgttctgg
     1021 gcgcatggct ttggcaaagt caccgataaa tttggcgtac cgtggatgat taatgtcatc
     1081 aaacaacagc c
//
LOCUS       DAZFRG010000533         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFRG010000533 DAZFRG010000000
VERSION     DAZFRG010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.536"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..213
                     /locus_tag="ACHKUC_005179"
     CDS             <1..213
                     /locus_tag="ACHKUC_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621215.1"
                     /translation="NYECCNYIIIAVSLLAGSSQYTLVSGPDGPLTRRYAPTSGKPSS
                     GPLRPRTKALSWLKAGMVWQGWGWVR"
     gene            375..512
                     /locus_tag="ACHKUC_005180"
                     /pseudo
     CDS             375..512
                     /locus_tag="ACHKUC_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            532..795
                     /locus_tag="ACHKUC_005181"
     CDS             532..795
                     /locus_tag="ACHKUC_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFO6621216.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSVQLRQQLQGRYQELRNEL"
     gene            785..>1090
                     /locus_tag="ACHKUC_005182"
     CDS             785..>1090
                     /locus_tag="ACHKUC_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6621217.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDKARRVNILAVKQAQDMDRRHQESE
                     "
BASE COUNT          287 a          266 c          293 g          244 t
ORIGIN      
        1 aattatgaat gttgtaacta catcatcatc gctgtcagtc ttctggctgg aagttctcag
       61 tacacgctcg taagcggccc tgacggcccg ctaacgcgga gatacgcccc gacttcgggt
      121 aaaccctcgt cgggaccact ccgaccgcgc acaaaagctc tctcatggct gaaagcgggt
      181 atggtctggc agggctgggg atgggtaagg tgaaatctat caatcagtac cggcttacgc
      241 cgggcttcgg cggttttact ccggtatcac atgtaaccac agcgtgccgc cttacatgcc
      301 gctggcgcgg cataagcata tgttggtggc tgtgtctgaa aggttatata ctctgcataa
      361 aacctgacaa cacgcagatt gccaggcaaa tgagcattat gcatccggtt atcgcctgcc
      421 tgtgaaacaa tatcaccggg gcgaatatcg atactctgac tcgttatacc catgcgtgtg
      481 gcatcaaaaa acctgcaatt gccctttatt gaatgaaagg gggagtcaat tatgaatagc
      541 accacacatt acgaaaacgc caatttcctg cgcgagctgg ccgaaagcct gcctcgtatt
      601 tttccagaag gcagtacaga caagtctgca ctattgcaac gcctcgctaa tgaagaactg
      661 gcacgggcgg aatacgacga acagatccga gcgaaagttg ccgctgctcg cgccgataag
      721 cggccgggaa tgagttccgt gcaactgcgc cagcaactac agggacgtta tcaggaattg
      781 cgcaatgagt tatgaaatag aatggcgacc aggtgcgatt gaggatataa cggcgttgtt
      841 tgaatatctg gccgagaatg catctctttg ggatgcaagg aacgtgacag agcgtctgat
      901 cactgcaacc gatcgtctgg cagattaccc caggctgtat gaaacagatg aacgctatgg
      961 cgaaggggtt cgccgtatta gcttacttgg gcagcatgta ctttatgaag tgaatgataa
     1021 ggcccgtcgt gtcaatatcc tggcggttaa acaggcacag gatatggatc gcaggcacca
     1081 ggaatcagaa
//
LOCUS       DAZFRG010000534         1082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFRG010000534 DAZFRG010000000
VERSION     DAZFRG010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.537"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..835)
                     /locus_tag="ACHKUC_005183"
     CDS             complement(62..835)
                     /locus_tag="ACHKUC_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6621218.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(837..>1082)
                     /locus_tag="ACHKUC_005184"
     CDS             complement(837..>1082)
                     /locus_tag="ACHKUC_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6621219.1"
                     /translation="TSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIIL
                     PGRIISPNTQLGPRVIVERNLPSGTYSLRQELIRTGD"
BASE COUNT          290 a          252 c          255 g          285 t
ORIGIN      
        1 caaaccgagt tatagctttt ctctgaaaac taccgaataa aatatactga tagactgctg
       61 cctagaattt ccagatcagg tgggctgcaa gttgcagacc gttataatca tcggttgggg
      121 tgttgaacca cactttacct gccgtcaacc cgagattaag ttgctcagtc agcggttgaa
      181 ttcgttgcaa actgatgctc ttcatgctgc cgtagcgtgt ttctggcgtc cagtcgtagg
      241 tgtaagcacc tgttccgcgg cttagccata gttgcgtata accagcaccg cgcgggtcct
      301 tcagacgcac ggaaatcata ttgctataac tactacctgc atcgctggag tcataatggg
      361 tatagcggta actggtgatc accgggccag tatagagtga tacgccgcct tgccaggcat
      421 tgacttcgac atcatcgtaa tatttagtgt agcgataacc ggtcgtgaaa agcgtttttg
      481 gcaacagggc taggttaata tcctgtcgaa aatcctggcg ggcaaagacc ggcgtattat
      541 ccgcaaaggc aatacccgtt cgggttgtca gccagttatt ccatttgtac cagactgcac
      601 cctggccgcg agtggcattg aaatgttcag tttcataatc acgacgcccc tgggaaagat
      661 taaaaagcag cgttgcgttt tcaacttttg ctaccagttc agcatacgct aaattacggt
      721 tgccatgttc gccagaataa tcggtaaaat cgtagcccgc ggtaatggaa gttaacccgg
      781 cataagcggg caatgcacta aaaaaaaacg gcactaataa cgtatgcttt atcattttaa
      841 tctcctgtac ggataagttc ttgtcggagt gagtaagttc cactaggtaa attacgttct
      901 acaatcacgc gcgggccaag ttgtgtgttc ggagaaataa ttcgcccagg caaaataata
      961 acttgtacac caaggcgtga acgcttcccg atataacaac ctaatttatc gcatccggta
     1021 gcgataattc cctctggagt tcgaacagac acgggttgtt catccagacg atgattactg
     1081 gt
//
LOCUS       DAZFRG010000535         1075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFRG010000535 DAZFRG010000000
VERSION     DAZFRG010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.538"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..228
                     /locus_tag="ACHKUC_005185"
     CDS             <1..228
                     /locus_tag="ACHKUC_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621220.1"
                     /translation="FNSKDKTTSNMIITHVMDHEFVCKLIDLAMRNAEKHLLKKAWKK
                     NAAEKLSEVDFKGVKQALAKMHYTVLAESIC"
     gene            302..544
                     /locus_tag="ACHKUC_005186"
     CDS             302..544
                     /locus_tag="ACHKUC_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6621221.1"
                     /translation="MIDEFHVMYMYKKIQAEAATTDIKTLEQLLKKFRSVVNERREEY
                     YQEIGEKKARKLKMKRLQRMLEKMACDRVSPEDLID"
     gene            594..1010
                     /locus_tag="ACHKUC_005187"
     CDS             594..1010
                     /locus_tag="ACHKUC_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621222.1"
                     /translation="MGWELHIVRTEHWFDSASAPISSEEWLQLVNDDKELSIDNKNGD
                     FFATWSGHCELDEPWLDWNDGRISTKHPDEALYCKMLQIAEKLHAVVVDEDDHKYSLP
                     SDLMNPSWANSSPTDKKTSLWGRLISKVKNSFLSVV"
BASE COUNT          371 a          159 c          245 g          300 t
ORIGIN      
        1 tttaactcaa aggataaaac gaccagtaat atgattataa cgcatgtaat ggatcatgag
       61 tttgtttgta agctaatcga tctggcgatg agaaatgctg aaaagcatct tctgaaaaaa
      121 gcatggaaaa agaatgctgc tgaaaaactg tctgaggtag attttaaagg ggttaagcag
      181 gcattagcca aaatgcatta tacggttctg gcggaatcaa tatgttgata tgttctgatg
      241 tctatcggcc ccttcttggg gctgataaca aatcttattt gtctgtccaa ggagcaaaaa
      301 aatgattgat gaatttcatg tgatgtatat gtataaaaaa atccaagcgg aagccgcaac
      361 tactgacatc aaaacacttg aacagctttt gaaaaagttc aggagcgtgg ttaacgaacg
      421 tcgtgaagaa tactatcagg agattggtga gaaaaaagcg cgtaagttga aaatgaaaag
      481 attacaaaga atgcttgaga agatggcgtg tgaccgagtc tcgccagaag atctcattga
      541 ctgataagtg ttctgtataa taacgtatct atatcaaaaa aatggaggtg gttatgggct
      601 gggagcttca cattgtaaga acggaacatt ggttcgatag cgcaagtgct ccaatcagta
      661 gtgaagaatg gctccagctt gttaatgatg ataaagaact atctatagat aacaagaacg
      721 gtgatttttt tgctacatgg agtgggcatt gtgaactcga tgaaccttgg cttgattgga
      781 atgatggaag gatttcaaca aaacatcctg acgaagccct ttactgtaaa atgttgcaga
      841 tagctgagaa gctacatgct gtagtcgttg atgaggatga tcataaatat tcgctaccaa
      901 gtgaccttat gaatccttca tgggcgaatt catcaccgac ggataaaaaa acatccttat
      961 ggggaaggtt aatttcaaaa gtaaaaaatt cttttttaag tgttgtttag ccagattcta
     1021 tctgatgcac taataattaa gaatgatgct agggaactaa aatgattata aatga
//
LOCUS       DAZFRG010000536         1069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFRG010000536 DAZFRG010000000
VERSION     DAZFRG010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.539"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..777)
                     /locus_tag="ACHKUC_005188"
                     /pseudo
     CDS             complement(1..777)
                     /locus_tag="ACHKUC_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            835..1020
                     /locus_tag="ACHKUC_005189"
                     /pseudo
     CDS             835..1020
                     /locus_tag="ACHKUC_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          297 a          252 c          243 g          277 t
ORIGIN      
        1 atgctgtttc cgctggaata ttcacctggc accagtccca gaaaagccat gagccgttca
       61 ggattggaaa atcgcgaaaa atcacccagt tcagcaacca gggttattgc gattagctga
      121 ccaacacctt taagagcctg caactggcag accagattgc acagagatca ctcaggtaac
      181 aggcgagata tttcctgatc gagttgaagt atgcgatctt caatctgctc cagattctga
      241 caataatgtt gaaagcaatc tgttgagata gcagaccaaa tgattgatta actaaccagg
      301 ttcgatgttt gccggttcag tgcttcctgg cataacgttt gtctgttcgt gtcacgtttt
      361 gcagcggcat gggctctgat taaatcccgc atagcttcat gagtcagctc agggagccag
      421 actgggggaa cctcaccatc cctcagtaac ctggcgagag aaattacgtc ccgatggtca
      481 tttttgatcc tgtctgcctg tgatttgggt atacgggaag ggggcaataa ccaggcattt
      541 tatattcatc actgtgagtt gatgatatag cccaaaacta cagggatcac cttcatagca
      601 ggaaagaatg tttccgtaag gtccctgcaa tttctgacat aatcgatgta cagccaggac
      661 ttcgttgtta atcgtaccat aataacgaac ttcgccaagc cattcaggtg tagcaatcgc
      721 caaggtttct ttatgaacat ccagcccgac gtaagctgca atattttccg aatccatgat
      781 gacctctgga atatcccacc aatgctatag tgagtatata aaggtcatat catctacgtg
      841 ttcttcggca acgatcacag tggagagcgt ggagcgctac tgtacgggct gattggcacc
      901 tgccgactga acagtatcga cccagaagcg tatttgcgcc atatcctggg cgtactactg
      961 gaatggcctt ccaaccgtgt tgacgaactc tcgccatgga atgtagtata ttaccaataa
     1021 ataaccgtca atacggcact cggttaacgc ttacaaataa gaatagaca
//
LOCUS       DAZFRG010000537         1066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFRG010000537 DAZFRG010000000
VERSION     DAZFRG010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.540"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1066
                     /locus_tag="ACHKUC_005190"
     CDS             <1..>1066
                     /locus_tag="ACHKUC_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6621223.1"
                     /translation="QTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTA
                     VEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTEL
                     LETQTVDFTLGSQGLRHTPGDIIEICDNDYAVTMTGGRVLSIDAASRTLTLDREVTLP
                     ETGTATVNLINGSGKPASVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFR
                     CVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGSLNSVIPPAVQHLTVEVSAA
                     DGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTL
                     TVRAINSYGQQGEPATTTFRI"
BASE COUNT          224 a          318 c          346 g          178 t
ORIGIN      
        1 cagacgctga cgttcgttca ggaccgtccg tcggatgtgg tgtggccgta caccaacagc
       61 gatgtggtgg tggatgataa cggcgtgggt ttccgctaca gcttcagtgc cctgaaggac
      121 cggcacacgg cagtggaggt gaattacacc gacccgcaga acggctggca gacctccacg
      181 gaactggtgg aagacccgga agccatactg cgctacggac gcaacctgct gaagatggac
      241 gcgttcggct gtaccagccg cggtcaggcc caccgtgccg ggctgtgggt gataaagacc
      301 gaactgctgg aaacgcagac ggtggatttc acgctcgggt cacaggggct gcgtcacaca
      361 cccggtgaca tcattgaaat ctgtgataac gactatgccg tgaccatgac cggcggacgt
      421 gtcctgtcca tcgatgccgc cagccgtacc ctgacgctgg accgggaggt gacactgccg
      481 gagaccggca cggccactgt taatctgatt aacggcagcg gtaagccggc gagcgtggcc
      541 atcaccgcac accccgcgcc tgaccggata caggtcagca ccctgccgga tggtgtggag
      601 acatacgggg tgtggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtc
      661 tccatccggg aaaacacgga cggcacgttc gccatcacgg cggtgcagca cgtaccggaa
      721 aaagaagcca ttgtggataa cggggccagc tttgagccgc agtcaggcag cctgaacagc
      781 gttatcccac cggcagtgca gcacctgacg gtggaggtga gtgcagctga cggtcagtat
      841 ctggcacagg cgaaatggga cacgccgcgg gtggtgaagg gtgtgcgctt cagtctgcgt
      901 ctgaccagcg gaagcggaga agacagccgt ctggtgacca ctgccatcac cgcagacacg
      961 gagcaccgtt tcagcggcct gccgctcggg gaatacaccc tgacggtgcg ggcgataaac
     1021 agctacggcc agcagggtga acctgccacc accaccttcc ggatta
//
LOCUS       DAZFRG010000538         1061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFRG010000538 DAZFRG010000000
VERSION     DAZFRG010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.541"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(117..810)
                     /locus_tag="ACHKUC_005191"
                     /pseudo
     CDS             complement(117..810)
                     /locus_tag="ACHKUC_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            854..>1061
                     /locus_tag="ACHKUC_005192"
     CDS             854..>1061
                     /locus_tag="ACHKUC_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621224.1"
                     /translation="MKTLPFLITANVLLITPPSTKSVRTSNDAIRKYIKKTVVINEDG
                     KQCVKTNLNFRKTDDGMVIYISALN"
BASE COUNT          305 a          236 c          235 g          285 t
ORIGIN      
        1 tagttgtaaa gcaagcacac tttgcatttc gagacaatcc aggtaaagac tcttaccacc
       61 cattttgtga tttttatact tatgatgata agttgaacgt tgggtagtgc ttccaatcag
      121 tagaacatgt gcttttcaat gaaggaaccg atgacttttt cgtggatttc aacggaacgc
      181 gagaagcaga ttgttttact taccagccgc ttgatgcggg tgcggagggt caggttgtta
      241 cgttcaatac gttgcgtgaa gattttgccg gtcaggtatt tcaccttcgg cacttctctg
      301 gcgtagctgc cccagtcatc gcggccacca tgccgataca gaacggagtc aacagggcta
      361 acaattcccg acaggcctca tcattccgtg gcccgaaagt gtaagccaac acaccgccag
      421 ttttagtgtt ctacgcatac cagagccagt gttgttgcac cttgttgcca acgaagctcc
      481 acagctcatc aagttcacag ataagggcaa catcagcgtg agctgactga cgcagatgtt
      541 atccgctggg gcgagagctt tttaatgtcc gtatgacggt attgatgctg attttcagtg
      601 ttctggcgat atcacggccc ctgtgccatt atgcgccatt tcaacgatca gctccttaaa
      661 gccgggtttt ctggcttcat aagagtaagt gagctggaaa acacgtttac atgaacgaca
      721 acggaaacgc tcatgctggg aacaactacg accatgtcga taaactttat cggaattaca
      781 gcgcggacaa tgaacagtaa cggtagccat ggaaaacctt aaataccagt agattaccag
      841 aaacttctat caattgaaga cattaccgtt tttgattaca gcaaatgttt tattaattac
      901 cccaccatcg actaaatccg ttagaacgtc gaatgatgcc attcgtaaat acattaaaaa
      961 gactgttgta atcaacgaag atggaaaaca gtgtgttaaa actaatctga acttcagaaa
     1021 aacagatgat ggtatggtga tatatatatc cgctctgaac c
//
LOCUS       DAZFRG010000539         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFRG010000539 DAZFRG010000000
VERSION     DAZFRG010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.542"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..>1059
                     /locus_tag="ACHKUC_005193"
     CDS             31..>1059
                     /locus_tag="ACHKUC_005193"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO6621225.1"
                     /translation="MNIQAVDIFCGAGGLTFGLKKAGIEVSHGIDIDESCRFAIESNN
                     PLTQFINQSVTELQSCDVSAMFKEGNIRLLAGCAPCQPFSKYRNPNSRKDDTKWRLLY
                     EFQRLVSDVMPELVTMENVPQLRNHKVFEGFVSALKTLGYHLWYDVVRCSEYGLPQNR
                     RRLILIGSQLGPISLDQKKVSRKVTVKDAIGRLPKVGAGEKLESDPLHRSPKLMDINL
                     KRIMHSLPGGTWDDWPEEIRADCHKKNSGATYKSVYGRMVWDDTSPTITTQCYGYGNG
                     RFGHPEQNRAITLREAAILQSFPMDYKFLDKETPFSFQKLGTMIGNAVPPIIGQIIGE
                     TFIRHVEGI"
BASE COUNT          310 a          178 c          256 g          315 t
ORIGIN      
        1 taataccagg ataaagtaac ggagaatctt gtgaacattc aggcagtaga cattttttgt
       61 ggtgcggggg gcttaacttt tgggctaaaa aaagccggga ttgaggtttc tcatggtatt
      121 gatattgatg aatcctgccg ttttgctatt gagagcaata atcctttaac gcagttcatt
      181 aatcagtcag ttacagaact gcaatcctgc gatgtgtctg ctatgttcaa ggaaggaaat
      241 attagattgc ttgctggatg tgctccttgc caaccgtttt ccaagtatcg taatccaaat
      301 agccgtaaag acgatacaaa gtggcgtttg ttatatgagt ttcaaaggct tgtaagtgat
      361 gtaatgccag agcttgtgac gatggagaat gttcctcaac ttagaaacca taaggttttt
      421 gaagggtttg ttagtgcatt aaagactctt ggatatcatt tgtggtacga cgttgtaaga
      481 tgttctgagt atggcttacc tcaaaataga cgtagattaa ttctaattgg ttcccaattg
      541 gggccgatca gccttgatca aaaaaaagtt agccgtaaag ttactgttaa ggatgctatt
      601 ggtaggttgc caaaagtagg ggcaggtgag aagctggaaa gcgatccctt gcatcgttcg
      661 cctaaattga tggatatcaa tcttaaacga atcatgcatt ctttacctgg tggtacgtgg
      721 gatgactggc ctgaagaaat tagagcagac tgtcataaaa agaattcagg tgctacctat
      781 aaaagcgttt atggacggat ggtttgggac gatactagcc ctacgataac tacccagtgt
      841 tatggatatg gaaatggacg atttggccat cctgagcaaa atcgcgccat aactttgcgt
      901 gaagctgcga ttttgcaatc ctttccaatg gattataaat tccttgacaa ggagacgcct
      961 ttttcattcc aaaaattggg gacaatgatt ggaaatgctg ttccccctat aattggtcag
     1021 ataatagggg aaacatttat cagacatgtt gaggggata
//
LOCUS       DAZFRG010000540         1044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFRG010000540 DAZFRG010000000
VERSION     DAZFRG010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.543"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..700
                     /locus_tag="ACHKUC_005194"
     CDS             170..700
                     /locus_tag="ACHKUC_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6621226.1"
                     /translation="MKKILLVAGAALALAGCGEKGDFEKAINTKIGQDKYCFSLDNNN
                     TSFPIRLAKPRLDSTGTGTNSVILDGFVEQGLMVFEQGYDSNVLGITEEGKKAKVWST
                     ADGACVGRRAVDEIKEWTEPGNGGQKVVRVSYTWKLNDVPSWIDKKAFANVKGMNEPA
                     DGAINLVKTSNGWKAN"
     gene            711..986
                     /locus_tag="ACHKUC_005195"
     CDS             711..986
                     /locus_tag="ACHKUC_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621227.1"
                     /translation="MENNTIKCPFCFKESQRGVHVCTGCLATVLYGTYPGWYAAVVLL
                     LTFGLSALSGMSTGMAGATISLPLIAIVGFVAGKVIFADNVVFWRRM"
BASE COUNT          282 a          196 c          279 g          287 t
ORIGIN      
        1 gtttttgatc gttttaaacg atatgacgag aagttaaaag aattgaatcc tatcaattgg
       61 ttaagcggtg gttttgccat gtgatatcag ccggatcccg caacaactct agccctgacc
      121 ggattaggtc aaaaatttag gcactttgat tatcaggatg gataagcaga tgaagaagat
      181 tttactcgtt gctggcgcag ctctggcact ggcgggatgt ggtgagaaag gtgattttga
      241 gaaagcgatt aataccaaga ttgggcagga caaatactgc ttctcgcttg ataacaataa
      301 caccagcttc cccatccgtc ttgccaaacc acgacttgat tcgactggca cagggaccaa
      361 ttcagtgatc ctcgatggct ttgttgagca gggcctgatg gtttttgaac agggctatga
      421 ttcgaacgta ttagggataa cagaggaagg caaaaaagcc aaagtctgga gcacagcaga
      481 cggtgcctgt gttggtcgtc gtgctgttga cgagattaag gaatggacgg aaccgggtaa
      541 cggcggtcag aaggttgttc gcgtatcgta tacatggaag cttaatgacg ttccgagctg
      601 gattgacaaa aaggcgttcg ccaacgttaa agggatgaat gaacctgctg atggtgcaat
      661 caatctcgtt aaaacaagta atggttggaa agccaattaa ggacgccatc atggaaaaca
      721 ataccatcaa atgcccgttt tgcttcaaag agagtcagcg tggggttcac gtctgtacag
      781 gctgtttagc gacggtgtta tatggcactt atcccggctg gtatgctgct gttgttttgc
      841 tgttgacttt tggtttatcg gcgttgagcg gtatgtcaac aggtatggca ggagctacta
      901 tcagccttcc gttaatcgct atcgtaggat ttgtagctgg taaggttatt tttgctgaca
      961 atgtagtgtt ttggcgaaga atgtagctag cagctataaa taataaacca ctaccaaccc
     1021 gcattgtggt ggtggtttat tatc
//
LOCUS       DAZFRG010000541         1040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFRG010000541 DAZFRG010000000
VERSION     DAZFRG010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.544"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..383
                     /locus_tag="ACHKUC_005196"
     CDS             <1..383
                     /locus_tag="ACHKUC_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621228.1"
                     /translation="LPVIFTHSVIKLSGISDFKIHSYIIKTSEYPEEFFSNAVWGKKN
                     IKPGEYYSVQAVSMFTTNQFILLCPKDIIRFYRESWKFDLLNVDFDTNTRKKLQEEAA
                     YCVPISAISVKRWDMPLQGSKPSN"
     gene            complement(738..856)
                     /locus_tag="ACHKUC_005197"
                     /pseudo
     CDS             complement(738..856)
                     /locus_tag="ACHKUC_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          341 a          192 c          204 g          303 t
ORIGIN      
        1 tattaccagt aatttttacc cattcagtta ttaagctctc aggtataagt gacttcaaaa
       61 tacatagcta cattattaaa acaagtgaat atccggagga gtttttttcc aatgcagtat
      121 ggggtaaaaa aaatataaaa ccgggggaat attactctgt tcaggctgtt tctatgttta
      181 cgacaaacca gtttattctc ctttgtccca aggatattat aaggttttat cgggaaagct
      241 ggaagttcga cctgcttaat gtagacttcg atacaaatac caggaagaaa ctccaggaag
      301 aagcagcata ttgcgtacca atttctgcaa tatctgtaaa acgatgggat atgcctcttc
      361 agggctcgaa accctcaaat tagaaggccg ctcgggcgac tgtattagat agtaatgcgg
      421 tatggaaaaa tagtgatggc aagatgaact tgacttggct aatcaaatac cggcgtagtg
      481 attaggaaac agggctcgga taacattatt gcgcggttca ttgaacctac tgatgacgcc
      541 ggatatatag tcgtaaaccg tttcgaacca gtacttatat caccaggaga gtccatatat
      601 gaccaccgtt tacagtaaaa acggtatctg ttttctgatc cttccagcgt caaactaccg
      661 ttgtaatggt gaggtctggt gctattgtaa taattcagaa gaaaatcgct gatttatttc
      721 cggacctcac ccttgcctgc tgttttgcag caagttttta tcacccacag agattcaatc
      781 atagatcgct tgttttggaa gtgacggttc ttattccgga gaatgctaca tgtgcagcac
      841 acgctgcatc aatcgatacc acttttatcc ccaataggca gaataacaaa acaagactgc
      901 gaattaaagt aatcagaaaa tgaatgagct agaagatact ggcaataaat caaggactaa
      961 acatataggg aataaaaata ataaaataac atcttatttt ttgtttttaa tgcatgagca
     1021 tatattttat attaataaca
//
LOCUS       DAZFRG010000542         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFRG010000542 DAZFRG010000000
VERSION     DAZFRG010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.545"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            104..>1024
                     /gene="cysB"
                     /locus_tag="ACHKUC_005198"
     CDS             104..>1024
                     /gene="cysB"
                     /locus_tag="ACHKUC_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO6621229.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALR"
BASE COUNT          257 a          247 c          251 g          269 t
ORIGIN      
        1 agtggttata gttagcacct tttttattat taaatcgtat tagtcacccg cctggtgtga
       61 cgaaaaaacg atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact
      121 tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc tcatcaacgg cggaaggact
      181 ttacacatca caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat
      241 tcaaattttt tcccgaagcg gcaagcacct gacgcaggta acgccagcag gacaagaaat
      301 aattcgtatc gctcgtgaag ttctgtcgaa agtcgatgcc ataaaatcgg tcgccggaga
      361 gcacacctgg ccggataaag gctcgctgta tatcgccacc acgcatactc aggcacgcta
      421 tgcgttacca aacgttatca aaggctttat tgagcgttat cctcgcgttt ctttgcatat
      481 gcaccagggc tcgccgacac aaattgctga tgccgtctct aaaggcaatg ccgatttcgc
      541 tatcgccacg gaagcgctgc atctgtatga agatttagtg atgctaccgt gctaccactg
      601 gaatcgggct atagtagtca ctccggatca cccgctggca ggtaaaaaag ccattaccat
      661 tgaagaactg gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga
      721 actggatact gcctttaacc gcgcagggtt aacgccgcgt atcgtcttca cggcaacgga
      781 tgctgacgtc attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat
      841 ggcggtggat ccggtcgccg atcccgacct tgtgcgtgtt gatgctcacg acatcttcag
      901 ccacagtaca accaaaattg gttttcgccg tagtactttt ttgcgtagtt atatgtatga
      961 tttcattcag cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt
     1021 gcgc
//
LOCUS       DAZFRG010000543         1021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFRG010000543 DAZFRG010000000
VERSION     DAZFRG010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.546"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..848)
                     /locus_tag="ACHKUC_005199"
     CDS             complement(144..848)
                     /locus_tag="ACHKUC_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621230.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDATQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTDDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          282 a          230 c          194 g          315 t
ORIGIN      
        1 tgaatccaca aacccatttt ttatttccaa taatacattt catacagcat ccttgttgat
       61 gaaataacat ctgttattca tactctatta aataatttca atctcaggtt acaacttttt
      121 gtaacctgaa atcgtattta tatttactcg tacagcacaa caaaacttgc tgttgactta
      181 actacacctg gtgcaacagt gctaccgttt tgagcataac ctgcagtaaa ggccgcgctg
      241 aaatcatggg tcgtagcatt tcctggaatt gtataagcgg cggtggaggc gctaccctct
      301 gccgcagtgg tattcaggtt aatcagacca ttattcgtcg tacctttgga gtaaattcga
      361 atccctacac cagtcgcccc cgcattgccg ctgttgtcag catccgttga tccttctact
      421 tcgttagcga gcgttttatt agcgctatct gcagtaccgg taaaacggaa ctcaattttc
      481 tgatcatctg tattgctaca ttcggtcaga ttaatattga aatcggtctg gttgctcgcc
      541 ccctgtgctt ctacgtcgat accagtgaaa aggttggaag gcacaaccgg caggacaact
      601 tcaatctgtt ttgaatcggt tgcgacctta caggtagctt tcactacacg gccagtaaag
      661 cggattttcc cccccattga atcaacgttg tcatccatat cagtgagcct gggataatca
      721 acggtgctcc agtcagtatc cgccccacca aattgtgttg catcgccggg tgctgcggtt
      781 accgcagtag acatcagcat acctgatgcc agtaatactg cggataaatt agataaattg
      841 aatttcattt atacctttcc ttttgttaaa aaatccaagc aacattggtg tttttacatt
      901 ctgcgttaat aagttatatc tcacaaaaaa aataagctga cgttggctaa ttccagaagg
      961 ttctttagaa gagtaattga gtgaataata ttatatatgc tgcttagtaa tgtacctaaa
     1021 a
//
LOCUS       DAZFRG010000544         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFRG010000544 DAZFRG010000000
VERSION     DAZFRG010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.547"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..824
                     /gene="gadX"
                     /locus_tag="ACHKUC_005200"
     CDS             <1..824
                     /gene="gadX"
                     /locus_tag="ACHKUC_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO6621231.1"
                     /translation="QPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVD
                     KCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQN
                     KNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTL
                     ARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYH
                     SVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          294 a          188 c          227 g          307 t
ORIGIN      
        1 tgcaaccact acatggtaat tgtctaattg cgtacgcaag acataaatat attctcacca
       61 tggttaatgg tgaatatcgc tattttaatg gcggtgatct ggtttttgcg gatgcaagcc
      121 aaattcaagt ggataagtgt gttgagaatt tcgtattagt ttcaagagac acgctttcat
      181 tatttctccc gatgctcaag gaggaggcat taaaacttca tgtacataaa aaagttcctt
      241 cattactcgt tcatcactgt accagagata ttcctgtttt tcaggaagtt gcgcaactat
      301 cgcagaataa gaatcttcgc tatgcagaaa tgctacgtaa aagggcatta atctttgcgt
      361 tgttatctgt ttttcttgag gatacgcagt ttataccgtt acttctgaac gtgttgcaac
      421 cgaatatgcg aacacgagtt tgtacggtca tcaataataa tatcgcccat gagtggacat
      481 tagcccgaat tgctagcgaa ttgttgatga gtccaagcct gttaaagaaa aaattgcgcg
      541 aagaaggtac gtcatattca cagttgctta ctgagtgtag gatgcagcgt gctttacaac
      601 ttattgttat atatggggtt tcaattaagc gagttgcagt atcctgtgga tatcacagcg
      661 tgtcgtattt catttacgtt tttcgaaatt attatgggat gacgcccaca gagtatcagg
      721 agcgatcggc gcaggaatta ccgaactgtg gctcggcggc aagtatggct gcgcaaggga
      781 ttttttacgg cactgaccgt tctgcggaag gaataagatt atagagtttt actcagacat
      841 aaaaaaaacc cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa
      901 ccgttataac actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac
      961 ttttactgga aataagatca gccatttttt tataaacata agctatacgc tgtgtg
//
LOCUS       DAZFRG010000545          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFRG010000545 DAZFRG010000000
VERSION     DAZFRG010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.548"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..>998
                     /locus_tag="ACHKUC_005201"
     CDS             54..>998
                     /locus_tag="ACHKUC_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6621232.1"
                     /translation="MYHHDFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGV"
BASE COUNT          304 a          171 c          246 g          277 t
ORIGIN      
        1 tttcttttaa taatgaaaat atttaattaa atctattatg taaaggggga atgatgtatc
       61 atcatgattt taatgaaaaa ataggtttct ggtatgtgat agccctcgca ggacagtcta
      121 atggtatggc ctacggtgaa ggtattcccc tgccagatac actcgacaaa cccgagtccc
      181 gtgtaaaaca actggctcgt cgaaaaacaa tcacaccagg aggaaaagag tgtaaattca
      241 atgagattat tccggcagat cactgcctgc atgatgttca ggatatgagt ggatatcacc
      301 atccggctgc tgatttgcat aaaggggaat atggttgtgt cggacaggga ttgcatatag
      361 ctaaaaagtt actgccatac atacctgagc aagcagggat attactggtt ccttgttgca
      421 gaggaggtgc cgcctttact gttggagctg aaggtatgta tgtacctgat acaggggcaa
      481 cagctgatgc aatgcggtgg ggcaccggaa cagcactcta tgaggactta gtagcccggg
      541 ttaaggtagc tctggaatat aacagaaaga ataaactgct atctgtctgc tggatgcagg
      601 gggagtttga tcttatgtca ccagattatg aaaagcatcc tgacttgttt tatcagatgg
      661 taacttcttt caggtctgag ttatctgaat actcatctca gtgtgttggt aattcgtcag
      721 aaagagttcc atggttgtgt ggtgatacaa cctggtactg gaaggaatct tatcaaaaag
      781 aatatgattt tatatatgga cattacagac aacggactga tgatgaaata cattttctca
      841 gctttcagga tagtaaccga catgaattaa ctaatgagcc agaagaagat gccgatgatt
      901 tatctgtcgg gtatctgggg tcatcatgga ggacagaatt aagctggaca acgtcacaga
      961 gaagtacaca ttttaattca atggccagaa ggggagtt
//
LOCUS       DAZFRG010000546          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFRG010000546 DAZFRG010000000
VERSION     DAZFRG010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.549"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..197)
                     /locus_tag="ACHKUC_005202"
     CDS             complement(<1..197)
                     /locus_tag="ACHKUC_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HFO6621233.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHN"
     gene            complement(243..>995)
                     /locus_tag="ACHKUC_005203"
     CDS             complement(243..>995)
                     /locus_tag="ACHKUC_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6621234.1"
                     /translation="DSSLFENTGDGIIYTGFVPLITVVEPVVSNQHSVLIIPGGGYRR
                     IVYEREGLDVGRWIAGQGYTAFILSYRMPSVNGYYSFADAKNALNFISYWAGMSERTF
                     KSVGCMGFSAGGHLAGYITHLHYEEETSVRPDWMALIYPVISLDRSIAHQGCRKRLLD
                     AGESEYSASLENSVTEYMPPVFIVHATDDKDVPVIHSLRYFNSIRDKGHHAEAHFFDK
                     GGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH"
BASE COUNT          315 a          241 c          159 g          280 t
ORIGIN      
        1 ttgttgtgaa ttgtattcca ggtgtttgac tcaaaacttg cccaccaacc attattccac
       61 ccctgactaa cctgaatcaa cgtttcataa gcgtgactgg cgcttctgta tcccccacgt
      121 agattaagag ttaatgcctg agagcacaaa ggtaaaccaa gaagaattac agttaatagt
      181 ggataatatt ttttcataaa tgcctcatca ttaaatatat ttatcacttg taatttgatt
      241 tctcaatgaa acttatgttc cgcaagccat tccagaagca ggctttgcca tttttctatc
      301 ggatatccat tttttccgga tacaccaaat ccatgacctc ccttatcaaa aaaatgagct
      361 tctgcatgat gtcccttatc ccgaatagag ttaaaatatc tcagagaatg aataactgga
      421 acatccttat catctgttgc atgcacaata aagactggtg gcatatactc tgtaacacta
      481 ttttcaagac tggctgaata ttcactttca cctgcatcca gaagacgttt ccggcatccc
      541 tgatgagcaa tactcctgtc aagagatata actggatata tcagagccat ccagtcagga
      601 cgcacagaag tctcttcctc ataatggaga tgagtaatat atccagcaag atgaccacct
      661 gccgaaaacc ccatacatcc aacactctta aatgttctct ccgacattcc ggcccagtaa
      721 gaaataaagt tcagggcatt cttcgcatct gcaaaggaat aataaccatt aacggagggc
      781 attctgtaag aaagaataaa tgccgtatat ccctgacctg ctatccatct gccaacatca
      841 agtccctctc tttcataaac aatcctcctg tatccacctc caggtataat cagcacacta
      901 tgttgattag atacaacagg ctcaacaact gtaataaggg gcacaaaacc tgtatatatt
      961 atgccatcgc cagtattctc aaatagcgaa ctgtc
//
LOCUS       DAZFRG010000547          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFRG010000547 DAZFRG010000000
VERSION     DAZFRG010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.550"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..890
                     /gene="rclR"
                     /locus_tag="ACHKUC_005204"
     CDS             36..890
                     /gene="rclR"
                     /locus_tag="ACHKUC_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO6621235.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          237 a          228 c          260 g          266 t
ORIGIN      
        1 gtgtttgtaa tcaaaaaacc actcaggagt ctgatatgga tgctcttagc cgtttgttga
       61 tgcttaacgc tccacaagga acgatcgata agaattgcgt gttaggaagt gactggcaac
      121 ttccacatgg tgccggggaa ttgtcggtta ttcgttggca tgcgttaacg caaggagcgg
      181 cgaagctgga aatgccgacg ggagagattt ttacattacg cccgggaaat gtggtcctgc
      241 taccacaaaa ttcagctcat cgcctgagtc atgtcgataa tgaatcgacc tgtattgtct
      301 gtggcactct tcggctgcaa cattcggcgc gttatttttt aacgtctttg ccggaaacgc
      361 tgtttgtagc accagtgaac catagcgtgg aatataactg gctgagggag gcgatcccgt
      421 ttttacaaca ggaatccaga tcggcaatgc cgggagtgga tgctctgtgt agccagatct
      481 gcgctacatt ctttaccctc gcggtgcgtg agtggattgc acaggttaat acagagaaaa
      541 acattctcag tttgcttctg catccacgtc ttggtgcggt aatacagcaa atgatggaaa
      601 tgcctggaca cgcctggacc gtcgaatcgc tggccagcat cgcccacatg tcccgggcaa
      661 gttttgccca gcttttccgt gatgtttccg gaaccacgcc gctggctgta ttaacaaagt
      721 tgcgtctaca aatagcggcc cagatgtttt cccgggaaat gctccctgtt gtggtgatcg
      781 ctgagtcagt aggctatgcc agcgaatcat cttttcacaa ggcgtttgtc cgcgagtttg
      841 gttgtactcc gggagaatat cgggaaaggg tcagacagct tgcaccctga ataaaaccgc
      901 cagaaatcag ggcaaagata atccgcattc cgggagttgt gagcctttca actatttcta
      961 tttccaacgg tagttttggg ctttatatat t
//
LOCUS       DAZFRG010000548          985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFRG010000548 DAZFRG010000000
VERSION     DAZFRG010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.551"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..487
                     /locus_tag="ACHKUC_005205"
     CDS             <1..487
                     /locus_tag="ACHKUC_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621236.1"
                     /translation="TTYSALSADSVIKISGRVLDYGCTVSSDSLNFTVDLQKNSARQF
                     PTTGSTSPVVPFQITLSECSKGTTEVRVAFNGIEDAENNTLLKLDEGSNTASGLGIEI
                     LDGNMRPVKLNDLHAGMQWIPLVPDQNNILPYSARLKSTQKSVNPGLVSASATFTLEF
                     Q"
     gene            500..>985
                     /locus_tag="ACHKUC_005206"
     CDS             500..>985
                     /locus_tag="ACHKUC_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621237.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSTVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NV"
BASE COUNT          298 a          213 c          221 g          253 t
ORIGIN      
        1 aaccacctat tctgctttat ccgcagatag tgttattaaa attagcgggc gcgtcctcga
       61 ttatggctgc acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag
      121 tgccagacaa tttccaacga ccggtagcac aagtccagtc gtcccttttc agattacatt
      181 aagtgaatgc agcaaaggga caacggaagt tcgggttgca tttaacggta ttgaggatgc
      241 agaaaataat actctgttga aactagatga aggaagcaat acggcctccg gtttgggtat
      301 agaaatactg gacggaaata tgcgtccggt gaaattgaat gaccttcatg ccgggatgca
      361 gtggatccca ttggtaccag atcagaacaa tattttgcct tactccgctc gtttgaagtc
      421 aactcagaag tccgtcaatc ctggactggt gagcgcttcg gcaaccttta cccttgaatt
      481 tcaataatca gagtcagata tgaaaagact tcacaagagg ttcctgttag ctacgttttg
      541 cacattattc acagcaactc tccaggccgc cgatgtcact atcactgtta atggtcgggt
      601 agtcgctaaa ccatgcacta ttcaaaccaa agaagctaac gttaatctcg gggatcttta
      661 tacacgcaat ctgcaacaac ctggttctgc atctggctgg cacaatatta ctttgtcatt
      721 aaccgattgt ccgattgaaa caagtacagt gacggcaatc gtgacaggtt caactgacaa
      781 tacgggttat tacaaaaatg aaggtactgc cgaaaatatt cagatagagc taagggatga
      841 ccaggatgcg acgttaaaaa atggcgatag caaaacggtt attgttgatg agatcactcg
      901 taatgcacag tttccactta aggcaagagc tatcacggtg aatggaaacg caagccaggg
      961 tacgatcgag gcgctaatca atgtg
//
LOCUS       DAZFRG010000549          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFRG010000549 DAZFRG010000000
VERSION     DAZFRG010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.552"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(177..809)
                     /locus_tag="ACHKUC_005207"
     CDS             complement(177..809)
                     /locus_tag="ACHKUC_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO6621238.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          246 a          219 c          201 g          314 t
ORIGIN      
        1 aaatgatttg tgataatatg ggattattgc ggtttacttt gaggaagata aaatgcgagt
       61 ttatagaacc gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa
      121 aaaattgtcg actggaggga gttaaatcct ttacctcaat atattgcctg tcaggtttag
      181 ttcacaaaca gtgcttcgct atcaacgatg attccatctt ccgcaccttt aagcgccagg
      241 tttgcctcgt agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga
      301 gtctgtttaa gcggcatcaa atattggttt tccattacac caacaccggc caggcgaagc
      361 tcttccattg cacccttttt atcccctttc gccatttttt cgttggcaat ttttatcgcg
      421 gcttcttttt cgggtgtcgc tacataactc tcagaaattc cgacagatgc attaatgaca
      481 atatattggt catcattgac gttggtcttt ttacctggtt tagcgaactt tgcccattcg
      541 gtactatcat ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttccgga
      601 tcgccatgaa acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt
      661 tcagagatac gctctgctgc tgccgttttc tgggtttgct taacttgctg agtttgctgt
      721 tgtgccgcta ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg
      781 accatcgtag ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa
      841 cgccgccatc atattgggtg agaatgaaat gagaaatcag agagcaacgg acattttgaa
      901 agggttatct tacaactgta gttattatcg caaagccgct tttagtctta cagttgtagg
      961 aatctgcttc ctgtcgcaga
//
LOCUS       DAZFRG010000550          979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFRG010000550 DAZFRG010000000
VERSION     DAZFRG010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.553"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(433..978)
                     /gene="hdeD"
                     /locus_tag="ACHKUC_005208"
     CDS             complement(433..978)
                     /gene="hdeD"
                     /locus_tag="ACHKUC_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO6621239.1"
                     /translation="MKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTVV
                     GALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAFI
                     AGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLVG
                     IELIFSAASLFSFASLFVKKQ"
BASE COUNT          335 a          222 c          193 g          229 t
ORIGIN      
        1 tgccagcttt cttttaacgc attgaaatta cctgattaaa taactcattc atgtcttcag
       61 gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa
      121 aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt
      181 gttcactttc ccacagcact tttaaaacat taaacctgac cacggaaaat atcagccatg
      241 ataatgtttg aaatggctaa tttgccatgg aacgaaaaaa atcagattaa attcagtagg
      301 ttgaaataat caacagcagg caattgtttc aatgatagtg cgtaattgaa ctgaaacact
      361 gtatagctaa gagagtatta actggatcat agtcacttaa tgtgactatg atccaggtgg
      421 aaatagggat aattattgct ttttaacgaa caaactggcg aagctgaaca gactggcggc
      481 gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttaccgaca ccatcggcgt
      541 agcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca
      601 actgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc ctgccacaca
      661 gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctctggtg cgcggatgaa
      721 gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa
      781 attatgactg cggttgctga atagcccgac aataagcgca atgcccgagc agattaataa
      841 tgcacccact actgtgctta aaatgtcgcc agagacgaac ggaaaactga tacacagcaa
      901 cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgtt ttttaagcat
      961 ctccagatca aacttcaaa
//
LOCUS       DAZFRG010000551          972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFRG010000551 DAZFRG010000000
VERSION     DAZFRG010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.554"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..>972
                     /gene="tdcA"
                     /locus_tag="ACHKUC_005209"
     CDS             106..>972
                     /gene="tdcA"
                     /locus_tag="ACHKUC_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO6621240.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASV"
BASE COUNT          266 a          189 c          224 g          293 t
ORIGIN      
        1 gccatggaaa gtattgaaag ttaatttgtg agtggtcgca catatcctgt tcatttcatt
       61 ttgatacact tcatgccgtc aatgaggtaa ttaacgtagg tcgttatgag cactattctt
      121 cttccgaaaa cgcagcacct ggtagtcttt caggaagtta ttagaagtgg ttctatcggc
      181 tcggctgcaa aagagttagg gttaactcaa ccggccgtca gtaaaatcat taacgatatt
      241 gaggattatt ttggtgtgga gttagtggtg cggaaaaata ccggtgtaac attaacacct
      301 gccggtcaat tgttactctc ccgttccgaa tccattaccc gtgaaatgaa aaatatggtt
      361 aatgagataa gcggtatgtc ttctgaggcg gtggtggaag tctcatttgg ttttccttca
      421 ttgattggtt ttacttttat gtcagggatg atcaacaagt tcaaagaggt gttcccgaaa
      481 gcgcaggttt ctatgtatga agcgcaactg tcttcgttct taccggcaat ccgcgacggc
      541 cgactggatt ttgcgattgg tacgttaagc gcagaaatga agcttcagga tttacatgtt
      601 gagccgctgt tcgagtccga gtttgtgctg gtagccagta agtcccgaac atgcaccggc
      661 accaccacgc tggagtcgtt gaagaacgaa cagtgggtgt tgccacaaac caatatgggg
      721 tactacagcg aactacttac tacgttacaa agaaatggca tcagtattga aaacatcgtt
      781 aaaaccgact cagtcgtgac aatttataat cttgttctca atgctgattt cttaactgta
      841 attccttgtg atatgacatc accttttggt tctaatcaat ttattactat tccggttgaa
      901 gaaacattac ctgtggcaca atatgccgcg gtatggtcga aaaattatcg tattaaaaaa
      961 gcagcatcgg tt
//
LOCUS       DAZFRG010000552          967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFRG010000552 DAZFRG010000000
VERSION     DAZFRG010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.555"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..>967
                     /locus_tag="ACHKUC_005210"
     CDS             132..>967
                     /locus_tag="ACHKUC_005210"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6621241.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTLHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGL"
BASE COUNT          308 a          133 c          247 g          279 t
ORIGIN      
        1 tttctccggt aaaggaaaag aatctggatg tattatcaaa acttgcagag cgactgttcg
       61 aagaacatga cagaaacaaa aaaatgaaaa taatctgaat aattgcaaga ttgttatata
      121 ggtgaaaatg aatgataaca ctggctgttg atataggagg aacgaaaatt tcagcggcgt
      181 tgatatctga tgacggttca tttttgctga aaaaacagat atcaacactt catgaaagat
      241 gtcctgacga aatgacaggg gctttacgtc tactggtttc tgaaatgaag ggaacagcgg
      301 agcgatttgc agtagcatca acagggatta ttaataatgg agtattgaca gctctgaatc
      361 cagacaatct tggagggctg aaagagtatc ctctgaaaaa tataatggaa gatataactg
      421 gtttaaatgg aagtgtgata aatgatgccc aggcagccgc atgggcagaa tatacagttt
      481 tgccgaaaga aatttgtgat atggttttta taaccgtttc tacgggggtt ggagggggaa
      541 ttgtagttaa cagaaaatta ttaacaggtg tttctggatt agccggacat gttgggcata
      601 tattgtccgg agtaacggat acagaatgtg gatgtggtcg tcggggatgc gttgaagcgg
      661 tttcatctgg tcgggccatc atgggggcag caaaaaataa acttgcagga tatagcacca
      721 aatatatttt tgaattagcg agacagggat ataaggaagc agaatttttg acagagcgtt
      781 ctgccagtac tattgctgaa ttaatagtaa gtttaaaatt actgcttgat tgtcaggttg
      841 ttgttgtcgg aggaagtgtt ggacttgctg atgggtatgt tcagaaagtg tcgaaacatc
      901 tttccatata ttctgaaatt tgtaatgtaa tgctgtttcc ggcatatttc agaagtgact
      961 ctggtct
//
LOCUS       DAZFRG010000553          960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFRG010000553 DAZFRG010000000
VERSION     DAZFRG010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.556"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            465..833
                     /locus_tag="ACHKUC_005211"
     CDS             465..833
                     /locus_tag="ACHKUC_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621242.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
BASE COUNT          258 a          260 c          237 g          205 t
ORIGIN      
        1 acacattgta aatcctcaga agaggcgctc gccatgctga gtacgctttg ttacaacctg
       61 atggtgacat gcggtatcca ttccatggac agccagatgc tcaatgggca tgggatatcc
      121 atgcaacttc aggggattga tattgccgaa aggccaggta agaatattca ctaacctgaa
      181 cagggtctga aaacgctgtc ttttcaggcc tctgcaggca aaaactgatg acaacaaaat
      241 ttctgcgttt gtaccccacc gatgaaaaag acaaaaactc acaccgggct tctagccagt
      301 aaagataaaa cccgacgtgt cagcctctac gaaacgccaa cggcatggtg tatcaggggg
      361 caggagtgct acagcaaaag tacaggacgc cgctgtggca gccacgacag tctgagcaga
      421 ctgcgcctgg acagcattaa gcctgttgaa tgagtggtgg caccatgaaa gataacagca
      481 caaccgccac cccagaagcc tggaaaatgc gttttctggc caacgggaca tccggcacat
      541 ggttttacac tgacgacatt acagtcactg acgcgtggct tcgcgcgcac ccggatgatg
      601 tggaggttat ccctctctgc cggataccac cccgtcagca ttcccatgaa caacatattg
      661 aagaactccg tgcagccctg gcccggatgc tgcatatgca tcatatgatg ctggaaacca
      721 cgcattacag ggaaaacatc accgatgccc gttgtatccg tgaaatgcat gaagctccat
      781 tacaggcgct caatattctg gccagatacc cgcataaaaa ggacgaggtg tgacgcatgt
      841 ggctactgat tttctggctt tcatctccct ttaatgtctc ttccggtgat gcccggcagc
      901 ccacactggc ggtgcacagc caggcatttc ataccgaacg ggcctgtcgc gatgcattgc
//
LOCUS       DAZFRG010000554          958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFRG010000554 DAZFRG010000000
VERSION     DAZFRG010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.557"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..878
                     /locus_tag="ACHKUC_005212"
     CDS             <1..878
                     /locus_tag="ACHKUC_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621243.1"
                     /translation="YLLLIAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPGQNLV
                     VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNV
                     LDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNII
                     SNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSARQ
                     VFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLGLSATYGQTGNKVA
                     AGAVQSVIGVTFIYE"
BASE COUNT          270 a          201 c          218 g          269 t
ORIGIN      
        1 tttatcttct attaattgca ggcaaagttt ttgccttcag ttgtaatgtc gatggcgggt
       61 caagtattgg agcaggtaca acctctgttt atgttaatct cgatcctgta atacaaccgg
      121 gccagaatct ggttgtagac ttgtctcagc atataagttg ctggaatgat tacggcggct
      181 ggtacgacac tgatcatata aacctggtac aaggatcagc tttcgcaggc tccctacagt
      241 catataaagg atcactgtac tggaataatg tgacctaccc tttcccgttg acgacaaata
      301 ccaacgtatt ggatattggt gataagacac cgatgccatt accgttaaaa ctctatatta
      361 ccccagtcgg agcggctggt ggagttgtca taaaagcagg ggaagttatt gcccgtattc
      421 atatgtataa aattgccacg ttaggaagcg gaaatccgcg taattttacg tggaatatta
      481 tttcaaataa cagtgttgtt atgccaacag gcggttgcac cgttgatagt cgtaatgtca
      541 ccgtgaatct cccggacttt cctggaagcg cagaaatacc ccttggtgta tattgctcta
      601 gcgaacaaaa gctctcattt tatctttcgg gtgccaccac tgacagtgcc cgacaagtgt
      661 ttgctaacac agcaccagat gcaacaaaag catcgggcgt gggtgtctct ctgatgcgta
      721 atggaaaaac actggcaaca ggagaaaatg tctccctggg gacagtaaat aaaagcaaag
      781 tacctctggg tttaagcgca acctatggac aaacaggaaa caaggttgca gccggagccg
      841 tccagtcagt cattggtgtc acttttatct atgaataatt gtgattttga cattagtcta
      901 tgcgctccat tcatgaaaat tgcgcggata attattttgt gaaggctatt atcctaca
//
LOCUS       DAZFRG010000555          954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFRG010000555 DAZFRG010000000
VERSION     DAZFRG010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.558"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            514..>954
                     /gene="alaE"
                     /locus_tag="ACHKUC_005213"
     CDS             514..>954
                     /gene="alaE"
                     /locus_tag="ACHKUC_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO6621244.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQV"
BASE COUNT          274 a          204 c          197 g          279 t
ORIGIN      
        1 attatatatt agccattttg atgagtaatt tcggttattg gtgaacagag ctgctctctc
       61 tgctcgataa ttccgcaagc ctgacaaaac caaagtgaat aaaaatgcaa ctcaaccgaa
      121 taaaagaaaa catctatcac tggtgctttc tgctgtcaat gtacattatc tggcataaaa
      181 aatgctgaaa atgacccggt cactacatac accgaattct ctacgcttat agcgttacct
      241 cacccccaaa ccttcatgag tgaaaataaa ggtaaacaca tatccaattg atttaattaa
      301 aaataaatca taccggcatg taaaaatcca caatgtacaa aaaacgcgcc gttacggaat
      361 attttatcta caaaaacaga ttaaataaaa atttttcact aattgattag tcatagccag
      421 cgatatacgc tatgcgaaaa tgcagatggc aatgagatcc actgctttca tctccattaa
      481 catcccatta cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt
      541 catgcagttg cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt
      601 gaagttttcc tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt
      661 ccggtgaaca tcttaattgc atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg
      721 gcacgcaaag ttagcccgtc gggctggata aaaaatctgg cggatatcct ggcttatgtg
      781 acgttccagt caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag
      841 attatggcgg cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc
      901 tacttcctcg attattgccg ccgactgttt aaagtcagcc gttaccagca ggta
//
LOCUS       DAZFRG010000556          951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFRG010000556 DAZFRG010000000
VERSION     DAZFRG010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.559"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..909
                     /locus_tag="ACHKUC_005214"
                     /pseudo
     CDS             75..909
                     /locus_tag="ACHKUC_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          297 a          228 c          203 g          223 t
ORIGIN      
        1 tcaacgaatg tcacagtgta agctcacaga gatattgcaa ttatctccgg acaagcgagg
       61 gaaatgctaa gttgatgcaa cgtatctgcg gcggatattt gaggcattac catgccaaca
      121 tctatgtgat gaaacacttt gttatacctg agattacaaa attaaggatg aaaaaatata
      181 cttctgggag aaacatgaga tcaatacagt tgcggaagtt tttgctgtat ccacacgaac
      241 gctgtattat ggcccagagg cacctatttc gcgtagcaaa gctccgctag tacatcgcac
      301 aaagcaatgg catctcgtaa ttctgtaaga aatacgtcga ctgagaatag agcttcccaa
      361 cctagaagaa cagatttttg tttgcttgaa gccgtggagt gaacagcacc gcctggtctg
      421 tccgagcatt tcaaccatcg gcaggatgtt cacggccgca cacgataaaa tgcgactgat
      481 accgatgtga ctgcctcccc aaggaaaaac actgttcata aaaaatacaa tcctccaacc
      541 cgcagatcaa aacgctatcg cccggtaaag agcagtgagc tcgttggtat agatgctatt
      601 gagctcagaa tgggcgagct gcgctgttat gtcattacca tgatcgacga acacagcaac
      661 tacgctctgg cacaggccgt gccgtcactt aactgtgata tcatcaaact cttcttttgc
      721 cgtgcagacc ggctattccc tatcgatatc aaccagggca tcgcagataa cgggaaagag
      781 ttcccaggaa acttcgacaa agcaccgcag gaatccgcta gcaagcccta tggacctacc
      841 cctttacgcc tagaacgcac ctctgaacgt tttaagcgaa cgccgaaaga acagttcatt
      901 gaatttaaat aaatagactc tttaaagagc taaacatatt taatcagaaa c
//
LOCUS       DAZFRG010000557          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFRG010000557 DAZFRG010000000
VERSION     DAZFRG010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.560"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..266
                     /locus_tag="ACHKUC_005215"
     CDS             111..266
                     /locus_tag="ACHKUC_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6621245.1"
                     /translation="MDTIELGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            268..396
                     /gene="ydfB"
                     /locus_tag="ACHKUC_005216"
     CDS             268..396
                     /gene="ydfB"
                     /locus_tag="ACHKUC_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6621246.1"
                     /translation="MDFDTIMEKAYEEYFDGLAEGEEALSFSEFKQALSSSGKSNG"
     gene            426..644
                     /gene="ydfC"
                     /locus_tag="ACHKUC_005217"
     CDS             426..644
                     /gene="ydfC"
                     /locus_tag="ACHKUC_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6621247.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAVQDLKWANEARTKQAE
                     NLSALEQDITRLEKAAALSIARMVTYPR"
     gene            667..>950
                     /locus_tag="ACHKUC_005218"
     CDS             667..>950
                     /locus_tag="ACHKUC_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621248.1"
                     /translation="MEFKDLPMPFQEMAANIVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENY"
BASE COUNT          303 a          205 c          226 g          216 t
ORIGIN      
        1 cgccatactg ttaacttaaa acatccgcgt tactaatatt tctatcaaga acagcacggc
       61 gctgtaggtt ttagttccgc cacccggcgt taaggggaaa tgaggtcagc atggatacta
      121 tcgagcttgg caacaacgaa tctctggtgt acggcgtgtt tcccaaccag gacggcacat
      181 tcaccgcgat gacgtatacc aaaagcaaaa cgtttaaaac cgaaaatggt gcccgtcgct
      241 ggctggaaag aaactcaggt gagtgatatg gatttcgaca caatcatgga aaaggcttac
      301 gaagaatact tcgatggtct tgccgaaggc gaagaagctc tcagcttcag tgaatttaaa
      361 caggcgcttt ccagttcggg aaaatctaac ggctgataag cgaaacagca ccgcgaggaa
      421 tcagtatgca gaaacgagaa cccgtcatca tcgcgccaga ctataccgat gatgaacttt
      481 atgagtggat gcgccagaaa attaatgcag tgcaggatct gaaatgggcc aatgaagcca
      541 ggactaagca ggctgaaaat ctgtccgctc tggagcagga tatcaccagg ctggaaaaag
      601 cagcggcatt aagcattgcc agaatggtta catacccacg ttaatagcta accaacgagg
      661 ctaataatgg aatttaaaga tttaccaatg ccattccagg aaatggcagc gaatatagtt
      721 cgttcccaac tggcgactct tgacctgagt accgtagaaa aagaaaccat cgatactata
      781 tccggtaacg tgcgtcgtgc ctttatcggt ctgtacgaag agaagcagct ctctgataac
      841 caggatttac atgaaaaata cttcctggat ctaatggaca tcattgataa ggggtttggc
      901 ttgttaatga aaaagaaagg gattcgaata gaaccccttg aaaattactt
//
LOCUS       DAZFRG010000558          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFRG010000558 DAZFRG010000000
VERSION     DAZFRG010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.561"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..715
                     /locus_tag="ACHKUC_005219"
     CDS             <1..715
                     /locus_tag="ACHKUC_005219"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6621249.1"
                     /translation="KIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSD
                     EITIQKEAIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDV
                     DYITPNETEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIH
                     SPAFPAVVKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEH
                     ESVIHRIQSIHYQQTIFTH"
     gene            729..>949
                     /locus_tag="ACHKUC_005220"
     CDS             729..>949
                     /locus_tag="ACHKUC_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO6621250.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLE"
BASE COUNT          314 a          203 c          195 g          237 t
ORIGIN      
        1 taagatttat aaaagtataa tttatcaaac gaaagaaacg caaaccggaa ctgccacgat
       61 ccttgttaat gaaggtactg gcgataatgt tattgcgatc tatcccggtg ccaacatgac
      121 aatgtcgtcc gatgaaataa ctattcagaa agaagcgatt attaattctg atgtcatact
      181 tctgcagctc gaaacaaatt ataccgctct gcaacaagcg atcacattgg cgcaaaaaaa
      241 cagtatcccg gtcattatta acccggcacc ctataatgat atcgtcaacg aacttattca
      301 ggatgttgat tatatcaccc ccaatgaaac agaagctggt ttactgtccg gcatagacgt
      361 acacgatctt gaatcagcta aacgagctgc agaagctatc cacaataaag gggtaaaaaa
      421 cacggttatc acgctcggta gcaagggctc gctcgcattt gatggcaaaa aattcattca
      481 ttcgccagcg tttccagcgg tcgttaaaaa tacggcagga gccggggatg cttttaacgg
      541 cgctctggca tcaggacttg cgaaaggaaa atcactggaa tcagcactgt gttatgccag
      601 cgcctttgca tccctagcgg tggagacaag caatgcttcc gacatgcctg aacatgaatc
      661 tgtgatacat cgaattcaga gtatacatta tcagcaaact atttttacgc attaagaagg
      721 gacagagaat gaataacacc aaaaaatcac taaaagtgct ctttattggg gaatcctggc
      781 atattcatat gattcattca aaaggatatg acagttttac ctcaagtaaa tatgaagaag
      841 gtgcgacctg gttactccaa tgtctgaaaa atagtcaggt agatgtcacc tatatgccag
      901 cacatacggt acaaattgcc ttccctgaag atgtggcaca gttagaaca
//
LOCUS       DAZFRG010000559          945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFRG010000559 DAZFRG010000000
VERSION     DAZFRG010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.562"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..549
                     /locus_tag="ACHKUC_005221"
     CDS             <1..549
                     /locus_tag="ACHKUC_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6621251.1"
                     /translation="LVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHL
                     TGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRHGQNLYLDNLEATGLYQVPL
                     SAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYSKKWQRRTHSVWRH
                     RHWHASAFTGICNDLAAASACM"
     gene            546..>945
                     /locus_tag="ACHKUC_005222"
     CDS             546..>945
                     /locus_tag="ACHKUC_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6621252.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYR"
BASE COUNT          174 a          256 c          311 g          204 t
ORIGIN      
        1 ctggtccaca gtcatcccgg tgggctgccc tggctgagcg aggctgaccg gcggctgcag
       61 ataaaaagcg cactgccctg gtggctggtc tgccggggtg acattcacaa attccgctgt
      121 gtgccacatc tgacgggacg gcgctttgag cacggggtga cggactgtta cacgctgttc
      181 cgggatgctt atcatctggc ggggactgag atgccggatt ttcatcgcga ggatgactgg
      241 tggcgtcacg gtcagaatct ctatctggat aatctggagg ccacagggct gtatcaggtg
      301 ccgttgtcag cggcgcagcc gggcgatgtg ctgctgtgct gttttggttc atcggtgccg
      361 aatcatgccg ccatttactg tggtgacggc gagctgctgc accatattcc tgaacaactg
      421 agtaaacggg agaggtattc caaaaaatgg caacgacgaa cgcattctgt ctggcgtcac
      481 cgccactggc acgcatctgc cttcacgggg atttgcaacg atttggccgc cgcctcagcc
      541 tgtatgtgaa cacggcagcg gaagccatcc gtgccctgtc gttacaggtg ccgggctttc
      601 gccgtcagat gaacgaaggc tggtaccaga tacgtattgc cggtgatgac acggcaccgg
      661 aggcggtgta cgcccgtctt cacgaacagc tgggtgaggg aacggtcatc cacattgtgc
      721 cgcgactggc cggagccgga aagggcggac tgcagattgt gctgggggcg gcagccatcg
      781 tgggctcttt cttcacggcc ggtgcctcga tggcgttatg gggttcagcc ctggcagccg
      841 gtggtttttc tgccaccacg atgctgtttt cactgggggc cagcatgata ctgggcggtg
      901 tggcacagat gctggccccg aaggcaaaaa caccggatta ccgcg
//
LOCUS       DAZFRG010000560          942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFRG010000560 DAZFRG010000000
VERSION     DAZFRG010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.563"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..854
                     /locus_tag="ACHKUC_005223"
     CDS             <1..854
                     /locus_tag="ACHKUC_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFO6621253.1"
                     /translation="GIEGDKSQIREWFGENTLTQMGNGAITTLHGVADLALVTFDALL
                     DTATATVACPIGEDGLCEQANINLNEKEQALFNISNSLINGQAWDALKKMIMDTNNGD
                     QIALEHFASFLWGFMIPAKIPEENISGKVFVEPVVLEGGAGGNWTVFDEVLDSNVIKQ
                     LTLTGCGAACGEMLLRDRYIFVTQNVIGTELTSMTSLANKLNKFDVGWEGNAVSESSL
                     YALSNTGSWGAMMWDSGSKVGHWVLVKGVDDAGNVIIYDPYQGSRYLMTEQEFKEVWN
                     GHSVYKP"
BASE COUNT          300 a          118 c          245 g          279 t
ORIGIN      
        1 atggtattga aggagataaa tctcagatca gagaatggtt tggagagaac acacttactc
       61 aaatggggaa tggagcaatc accactttac atggtgtggc ggatttagcc ttagttacat
      121 tcgatgctct gttagatacg gcaacggcaa cggttgcttg cccgataggc gaagatgggt
      181 tatgtgaaca ggcaaatata aatttaaatg aaaaagaaca ggctttattt aatattagta
      241 attcattgat aaatgggcaa gcatgggatg cacttaaaaa aatgataatg gatacaaata
      301 atggtgatca gattgctctg gagcattttg ccagcttttt atggggcttt atgatacccg
      361 caaaaatacc agaagaaaat atctctggga aagtatttgt agagccagta gttttagaag
      421 gtggtgctgg tggcaattgg acagtatttg atgaggtttt agattcaaat gtaataaagc
      481 aattgacttt aacaggctgt ggagcggcct gtggggaaat gttgcttagg gataggtata
      541 tttttgtcac gcaaaatgta attggaacag aattaacatc tatgacgtcg ctggcaaaca
      601 agcttaataa atttgatgtc ggctgggaag ggaatgcagt gagcgaatct agtttatatg
      661 ccctcagtaa tactggttca tggggagcaa tgatgtggga ttctggaagt aaagtagggc
      721 attgggtact tgttaagggt gttgatgatg ctgggaatgt gataatatat gatccatatc
      781 aagggagtcg atatctgatg actgaacagg aatttaagga ggtatggaat gggcatagtg
      841 tttacaaacc ataatattga tttgttatcg gttgaatttg atgagattac aaaaaattgt
      901 aattacactt ttagtgtaga tggagagacg gctatcttta ct
//
LOCUS       DAZFRG010000561          942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFRG010000561 DAZFRG010000000
VERSION     DAZFRG010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.564"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..>942
                     /locus_tag="ACHKUC_005224"
     CDS             102..>942
                     /locus_tag="ACHKUC_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621254.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPTPPKPISFNNDVILDKTEKTLTIRD
                     SVFTYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGTTGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATV
                     DNKG"
BASE COUNT          285 a          218 c          221 g          218 t
ORIGIN      
        1 taacaatcta ctggcaatat tggacgtcct ctatgtttta aataatcaat tggtcgggtc
       61 agggtatccg accttcttta tattcgccag aaggatttat tatgcaaagg aaaactctat
      121 tgtcggcctg tattgcatta gctctgagtg gtcagggttg ggcggcagat attacagaaa
      181 tagaaaccac cacaggtgaa aagaaaaata ccaatgtgac ttgtccagca gatcctggaa
      241 aactcagtcc agaagagctt aaacgcttac cctctgaatg ctcctctgta gtcgaacaga
      301 acctgatgcc atggcttgtc acaggtgctg ctactgcgtt aatcacaacc ttagccatag
      361 tggaactaaa cgacgatgat gatcatcatc gcaataattc tccacttcca ccaactccac
      421 ctgatgatga ttcagacgac actccagttc ctccaactcc tggcggagat gagataatac
      481 cagacgatgg tccggatgat accccgacgc ctcccaaacc gatttcgttt aataatgacg
      541 ttatcctcga taaaacagaa aaaacgttaa ctattcgcga ttcagttttt acttataccg
      601 agaatgctga cgggactatt tctctgcaag atagcaatgg gcgtaaggcg actattaatc
      661 tttggcagat tgatgaaacg aataacacgg ttgcccttga agggatgagc gcagatggtg
      721 caacgaagtg gcaatataat cacaacggtg agctcgttat tacgggcgac aataccacag
      781 taaacaacac tggaaaaacc atcgtcgacg gcaaaggcac caccggaact gaaatcgcgg
      841 gtaacaacgc ggtggtgaat caggacggcg agctggatgt gagcggcggc ggtcacggta
      901 ttgatatcac cggcgacagc gcaacggtgg acaacaaagg cg
//
LOCUS       DAZFRG010000562          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFRG010000562 DAZFRG010000000
VERSION     DAZFRG010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.565"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..593
                     /locus_tag="ACHKUC_005225"
     CDS             <1..593
                     /locus_tag="ACHKUC_005225"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFO6621255.1"
                     /translation="GTALSKAGISLNRMSEDIDIKLVPTENFMQNGRDKRRKIRKEIV
                     QIITDVIHNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMEST
                     LLEHPESRDIYSLVTELTGKGTPVTAFPCATILSTQAEKLISMMRRTAAHLRNPEQQD
                     DEFLVRHIYDNYCIVREKGVNVPVLKNFVQICIQLW"
BASE COUNT          310 a          195 c          191 g          236 t
ORIGIN      
        1 gaggtaccgc attatcaaaa gcaggtatct cgctaaacag aatgtcagaa gatattgata
       61 taaagctcgt gccaacagag aactttatgc aaaatggcag agacaagaga agaaaaattc
      121 gcaaggaaat cgtgcaaata atcacggatg ttattcataa ttctgacatt ttcagcttgg
      181 ataatgaaaa cgcccgtata acacgtgatg agtatcgtta taacgaaatc tcggtacgtt
      241 atccccagac atttgcacaa gtcccctgtc ttcgtccatt tattaagctg gagttgatgg
      301 aaagcactct gctggaacat ccggaaagca gagatattta ttcccttgtc acggagttaa
      361 cgggtaaagg tacacctgta acagcattcc cctgtgccac cattttatcc actcaggctg
      421 aaaagctcat ctccatgatg cgaagaacag cagcacatct tcgtaatccc gaacaacagg
      481 atgatgagtt tctggtacgt cacatttacg acaactactg cattgtcagg gaaaaaggtg
      541 tgaatgttcc tgtattgaaa aattttgttc agatatgtat tcagctatgg taaccagtac
      601 ccacaatttt gtagctcacc agttgatgaa cttaagaaag ggctggatgc actccgggat
      661 tatcctgtac acaaattgcg ctttcaacga tttgttaaac cgatggtatt tggtaataca
      721 cagattaact gggaagaagc ctattccagt ttcagacaaa cagcgctaag tgttctgacc
      781 agctaacaaa cgcgttaagt acgattccac aattcagcat atcgaaccag accaaatgat
      841 tacagcaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt gcacatgggg
      901 gcctcattgg tggcctcaaa aacaacaaat ac
//
LOCUS       DAZFRG010000563          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFRG010000563 DAZFRG010000000
VERSION     DAZFRG010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.566"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..437
                     /locus_tag="ACHKUC_005226"
     CDS             <1..437
                     /locus_tag="ACHKUC_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6621256.1"
                     /translation="VFTNKVQYPDGKPPVWAKCTKGVYSSYWEEWKKVREVVKVACPE
                     CGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAIC
                     EVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            581..656
                     /locus_tag="ACHKUC_005227"
     tRNA            581..656
                     /locus_tag="ACHKUC_005227"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Ile,seq:cat)
     gene            757..830
                     /locus_tag="ACHKUC_005228"
     tRNA            757..830
                     /locus_tag="ACHKUC_005228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:791..793,aa:Arg,seq:tct)
BASE COUNT          245 a          188 c          258 g          240 t
ORIGIN      
        1 tagtttttac aaacaaggtc cagtatccgg atggtaagcc gccggtatgg gcaaagtgta
       61 cgaaaggtgt gtattcgtct tactgggaag aatggaaaaa agtcagggag gtggtaaaag
      121 ttgcctgtcc ggagtgtggc ggaaagggtg aggtttccac cgcctgtaag gattgccgtg
      181 ggcgtggtgt cgccattcat cgtgaagagt cggtaaaacg tggtatgcct gttatcagag
      241 actgccagcg ttgtggtggt cgtggctatg aaagactacc atcaacggag gcatttaatg
      301 ctatatgcga ggtgacaaac cagataacac gcgcgtcatg ggaaaaaaca gttaagaaat
      361 tctatgatgc gctggtgacc cggtttgata ttgaagaagc atgggctgag cggcagttaa
      421 aaaaggtaac taggtaacaa ggttgatttt tccggaatct gtggtaaatt cgtcataaca
      481 atgggctttt tatgcctgac gttagaagag tttctacaac ccgccgccga gcgggttttt
      541 tattgcggaa ttaattacgg accgttatta ttctgctccc ggccctttag ctcagtggtg
      601 agagcgagcg actcataatc gccaggtcgc tggttcaaat ccagcaaggg ccaccatcac
      661 aaaccgccat tagcttatca ggaagagcag acgacacgat aacagggttg ttggtgcggg
      721 ggcgggtccc cgatggcggt ccattatcgg tattcagcgt tgttagctca gccgggcaga
      781 gcaattgcct tctaagcaat cggtcactgg ttcgaatcca gtacagcgcg ctatattcat
      841 tcttccagat tccttccggc agagccttat actgaaatat acctggctca ggatattgtt
      901 gaaaatattt tatgtttgtc aaaaataaaa g
//
LOCUS       DAZFRG010000564          930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFRG010000564 DAZFRG010000000
VERSION     DAZFRG010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.567"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..235
                     /locus_tag="ACHKUC_005229"
                     /pseudo
     CDS             81..235
                     /locus_tag="ACHKUC_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540156.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
     gene            507..930
                     /locus_tag="ACHKUC_005230"
                     /pseudo
     CDS             507..930
                     /locus_tag="ACHKUC_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          248 a          229 c          225 g          228 t
ORIGIN      
        1 atgactggat gagcggagcc tttcaggtta aggttttcat atatccaggg tgatgacacc
       61 ggctctatga gataaactaa ataaattcat tacttattcc cctgcagaat tcaccagaac
      121 gcggctcctt gttacccatt gcgccggtgg ggattttgca acagcgctct ccctgagaag
      181 aatggccact tccacgggga ccaaaccggc ctacctgctg gccccggaag tgagttagct
      241 ggcctagatg taaccatccg ttcaactcaa ttctcttagc gtgggaaaat ttccgaattc
      301 caagctgacc ccataaagtg cagatattga ttacgactct gaatcattgc atctcccgtt
      361 aatttttaac ttgtcccatt ttcgtcatta tcactaacag atgccgcgtc agcggtagac
      421 tagcccccag aatctccaga caaccaatat caattaaata agtgatagtc ttaatactag
      481 tttttagact agtcattgga gaacagatga ttgatgtctt aggaccggag aaacgcagac
      541 ggcgtactac acaggaaaag atcgcaattg ttcagcagag ctttgaaccg ggaatgacgg
      601 tctcccttgt tgcccggcaa cacggtgtgg cagccagcca gttatttctc tggcgcaagc
      661 aataccagga gggaaatctt actgctgtgg ccgcaggaga acaggtcgtt cctgcctctg
      721 aacttgctgc tgccatgaag cagattaaag aactccagcg cctgctcggc aaaaaaacga
      781 tggaaaatga actccttaaa gaagccgttg aatatgggcg tgcaaaaaag tggatagcgc
      841 acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc tccgggtgtc
      901 gcgtgcgcag ttgcacgtca ttctcagacg
//
LOCUS       DAZFRG010000565          925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFRG010000565 DAZFRG010000000
VERSION     DAZFRG010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.568"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..728)
                     /locus_tag="ACHKUC_005231"
     CDS             complement(<1..728)
                     /locus_tag="ACHKUC_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6621257.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAI"
BASE COUNT          293 a          185 c          143 g          304 t
ORIGIN      
        1 tgaattgcgc gctgtaacag gatgtccttt tcaagaaact gttcatagat atcaccaata
       61 tctttctgtt ttattttttc attgcataac acgcgacttg aaaaatattt aaggtgcatc
      121 ataaatctgg aatagttaaa tgaacttctg tccacctttc tgttataaac aacttcaata
      181 agccccgtga ttatttgcga caactcaata atccgtactg attcataatc aactgaattt
      241 tcataattat ttaaaatatg caatgctata aaaccaatct catcatcagg aagttgaacg
      301 ctgaattgct cattaatgat agttaatgca tcgcgactta tcgcatactc tgctttgtac
      361 aacgctttga tttcatcaag gaaaacatta ttaatttgta tacctgaaac gactcgggat
      421 attgcattcg aaatgtgatc cgcaagaatc aatagtagcg atggattgag atttttttcg
      481 agatttttct ccgcatattg cactattttt tcggcaacaa acacatactc aatatctaca
      541 tgttcaatca atttataaag tttgtttttt tgttcatttc tgacataaaa aatccggtta
      601 gccggatgct cagggaccga cattccatat tttttgttgt aaccgacgcc aggcccagaa
      661 atgataacct cctgtccatt catcgatgcc tgtacacaat tattgttcat gactttttcg
      721 atgatcattc catggctcca aaaaaaaagg caaggcacgt cagaagtaat aactacttca
      781 aacattgcct tgcctgattt aacagttaca agatgattaa ctaatgcgta tacattacac
      841 ggaattgtgc ttaattaaag agatggtcgg cttatttttc aagccgacat tcaaatatcc
      901 gacatcagtc acaaaaccag cgtct
//
LOCUS       DAZFRG010000566          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFRG010000566 DAZFRG010000000
VERSION     DAZFRG010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.569"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            194..346
                     /locus_tag="ACHKUC_005232"
                     /pseudo
     CDS             194..346
                     /locus_tag="ACHKUC_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            357..872
                     /gene="ogt"
                     /locus_tag="ACHKUC_005233"
     CDS             357..872
                     /gene="ogt"
                     /locus_tag="ACHKUC_005233"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO6621258.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKESYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT          227 a          204 c          235 g          250 t
ORIGIN      
        1 atttttttat tcatttttta gctctgtaaa taaccacatt ctcgccggga ttggggaatg
       61 agtgacgaga tcagtggtgt ctggcggcta tcacgccaca gaatcaaata acggatagcc
      121 tacggtaaaa tttcgctgac tggaatttga tggcttgccc tcatttcttc aggcagaaaa
      181 atcaaaaaat cgctttatgc ttaaatactt cgacaaacca gacgctaaac ctggtactta
      241 ctttttgtta gtattaatct tttcggtggt atggctgctg atgttgctgg cgtggtatct
      301 tgttggcttg ccgataggtc cgggtattta cccacgtttg tcttaagaga gaacggatgc
      361 tgagattact tgaagaaaaa attgccacgc cactgggtcc actgtgggtg atttgcgatg
      421 agcaatttcg cctgcgggcg gttgaatggg aagagtacag cgaacgcatg gtgcagctgc
      481 tggacatcca ttatcgcaaa gaaagctatg agcgcatttc tgccaccaac ccaggtggtt
      541 taagcgacaa gcttcgtgaa tattttgccg gtaatcttag cattattgat acgcttccca
      601 ctgccacggg ggggacgcca tttcagcgcg aagtctggaa aacactgcgc actatcccct
      661 gcgggcaggt aatgcattat ggccaactgg ctgaacaatt aggccgccct ggcgcggcgc
      721 gtgccgttgg tgcggcaaac ggatcgaatc ccatcagcat cgtcgtacct tgccatcggg
      781 ttattggccg aaacggcacc atgaccggat atgcaggcgg agttcagcga aaagagtggt
      841 tattgcgcca cgaaggttat cttttgctgt aaacattaaa caatttgtgc cagcttgttc
      901 acacttttat gtaaag
//
LOCUS       DAZFRG010000567          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFRG010000567 DAZFRG010000000
VERSION     DAZFRG010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.570"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..684
                     /gene="ydeO"
                     /locus_tag="ACHKUC_005234"
     CDS             <1..684
                     /gene="ydeO"
                     /locus_tag="ACHKUC_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO6621259.1"
                     /translation="DGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTV
                     TVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLL
                     TNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDAR
                     MQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTGMNT
                     DNTMNALAI"
BASE COUNT          283 a          171 c          181 g          273 t
ORIGIN      
        1 gacggcgaga tcctgatggt agataacgct gttcgtacgc attttgaacc ttatgagcgg
       61 cattttaaag aaatcggatt taatgaaaat accattaaaa aatatctaca atgcactaac
      121 atccagacag tgacggtgcc tgttcctgcg aagtttttac gtgcttcaaa tgtaccgact
      181 ggattgctta atgaaatgat tgcttatctc aactcggaag aacgcaatca tcataatttt
      241 tcagaacttt tgcttttttc ttgcctgtct atttttgccg catgcaaagg tttcattaca
      301 ctattaacta acggtgtgct atccgtttcc gggaaagtga gaaatattgt caacatgaag
      361 ctggcgcacc catggaagct gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg
      421 ttgaagaaaa aactaaagca agagcaaacg acattctcac agattctttt agatgccaga
      481 atgcagcacg caaaaaattt gatacgcgta gaaggttcag tcaataaaat tgccgaacaa
      541 tgtggttatg cgagtacatc ttattttatt tatgcgttcc gcaaacattt cggcaacagt
      601 ccgaagagag tttctaagga gtaccgttgt caaaggcaca cgggtatgaa cacggacaac
      661 acgatgaatg ctttagctat ttgattattt gctaacgagt agtcaaccac acacgctgcg
      721 taagaattaa atggggcagc cattccctgc cccgcgttgt ttttaggcga tatatttctt
      781 gaaataaata agtgacagtc atcacatatt tatgcatttg cataacctgt tgcatgatta
      841 tttatgatct cagttcggca ttttggcagt aaaatacaat aatttattaa atatcaatag
      901 attagttg
//
LOCUS       DAZFRG010000568          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFRG010000568 DAZFRG010000000
VERSION     DAZFRG010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.571"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(40..819)
                     /locus_tag="ACHKUC_005235"
     CDS             complement(40..819)
                     /locus_tag="ACHKUC_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6621260.1"
                     /translation="MRQYRLWLAMIKVTIVLFLLIFPTAQAGLVIGGTRFAYPENKSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWVRKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     SQGVCEITWQTLDIYNNVLPAWKVNMNPLQMDVLGSQVKTD"
     gene            complement(785..>900)
                     /locus_tag="ACHKUC_005236"
     CDS             complement(785..>900)
                     /locus_tag="ACHKUC_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFO6621261.1"
                     /translation="TPSVDNILHFFAAYKALKNNVQPGDANASVSFMVSYD"
BASE COUNT          269 a          183 c          164 g          284 t
ORIGIN      
        1 ctttatatcc ggcataagta atcatatgat ttccttttac taatccgttt taacttgtga
       61 gcctaacaca tccatctgca acggattcat atttactttc catgcaggca atacattatt
      121 ataaatgtcc aatgtctgcc atgttatttc acaaactcct tgcgatttac accatgattg
      181 ttggcggtgg ctaaatggtg cgatcatacc accatccata actttaccat caacatgaag
      241 gttatagagt gtcacatacc tggggcctgg attataaacg gtaacaattt cattttttcg
      301 tacccagcgc aatttttgat atgacatatt ggggtcttca gacaaatttg atggccgata
      361 gaatattttc atacgattgc ggaccgctat ttgcaattga ttatcagttt tggttggatg
      421 actattttcc aacgatggta atgcagcaac attaagtata aataaacttt cccggtcggc
      481 aggtaaacta ccaccgacac gattaattct tatttttgta tttttgtcag gcttcaaaac
      541 aaacagtggc ggagttgcga taaataccgt tccagaaaat gagggcaaag attcaaccgt
      601 tcccgtcaac tgacgagcat catcagggga gactgcaacc ttgactaaca tatctctgtc
      661 tgaagtgttt ttgagttcaa cagatattga ggacttattt tccggatacg caaaacgcgt
      721 tccaccgatt actaatcctg cttgtgcagt aggaaatatc aggaggaata atactatggt
      781 aaccttaatc atagctaacc ataaacgata ctgacgcatt tgcatcccct ggttgtacat
      841 tattttttaa tgctttgtaa gccgcaaaaa aatgcaatat attatcaacc gatggtgtaa
//
LOCUS       DAZFRG010000569          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFRG010000569 DAZFRG010000000
VERSION     DAZFRG010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.572"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHKUC_005237"
     CDS             <1..153
                     /locus_tag="ACHKUC_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO6621262.1"
                     /translation="LNQLVESNEKELKDCNLLGKRDKRRAKILASKNYILWRMVRLVN
                     RKKNKR"
     gene            199..>899
                     /locus_tag="ACHKUC_005238"
     CDS             199..>899
                     /locus_tag="ACHKUC_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFO6621263.1"
                     /translation="MSFCWNEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKR"
BASE COUNT          280 a          150 c          153 g          316 t
ORIGIN      
        1 ttaaatcaat tagttgaaag caatgaaaaa gaattgaaag attgtaatct tcttggcaag
       61 agagataaga ggcgagcaaa gatattagca tcaaaaaact atattctctg gaggatggta
      121 agactggtaa atagaaaaaa gaataaacgt taggtcttgc attaaataac ccatcctcgt
      181 agcataggtt gaaattatat gtcgttttgt tggaatgaaa ttaactctgg tgtcaagtct
      241 ttaattctca tattatgtat gttgtcttta atgactttgt ctttgtggga tgatgttgca
      301 acaaagtttc ttcatgcagc tggaattata tctgcattat attttctagc gacaccaaaa
      361 aaaacaataa ctaataatcc tactttgtta attttcatct cattatgtct tttgggtatc
      421 gttaatatca tctggtattc acattataaa gtttcaggct cggtttatac caatgcatat
      481 cgtggcccaa tggaaactgg aaaaattgcc ttgtgtagtg cttttatttt cttagttctt
      541 tttgctaaag atgagatgag aaccaaaata aaatttggga aactaattct gttcgcatcc
      601 ttggcaacgc agttactttt ttttgcgcat gccatgtggc aacatttcta tttaaacgtc
      661 gaccgtgttg cattatcagc ttcccacgct acaacagcag gctacatcat ccttttccct
      721 tctttactgg catcaattct cattttaaaa tccgacttta gacataaaac aacattatat
      781 acaattaact tcatgcttag cttatgtgct gttatagtaa ctgaaacgcg tgcagccata
      841 ttagtgtttc cattctttgc gttactatta atcgtaatgg atagttatat taataagcg
//
LOCUS       DAZFRG010000570          887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFRG010000570 DAZFRG010000000
VERSION     DAZFRG010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.573"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..287
                     /gene="ycgZ"
                     /locus_tag="ACHKUC_005239"
     CDS             51..287
                     /gene="ycgZ"
                     /locus_tag="ACHKUC_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO6621264.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            330..602
                     /locus_tag="ACHKUC_005240"
     CDS             330..602
                     /locus_tag="ACHKUC_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO6621265.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
     gene            631..>887
                     /gene="ariR"
                     /locus_tag="ACHKUC_005241"
                     /gene_synonym="ymgB"
     CDS             631..>887
                     /gene="ariR"
                     /locus_tag="ACHKUC_005241"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO6621266.1"
                     /translation="MLEDTTIHNAISDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTA"
BASE COUNT          305 a          146 c          184 g          252 t
ORIGIN      
        1 ctattgttac ctcacttaac attgattaac atttttaaca gaggcgtagc atgcatcaaa
       61 attcagtgac ttcagattct gcaggagcaa tcactcgtta ctttgcaaaa gctaacttgc
      121 ctactcagca ggaaactctc ggggaaattg tgactgagat tttgaaagat ggacgtaatc
      181 tgagtcgaaa gtcgctttgt gccaaacttc tttgccgact ggaacaagcg acaggggaag
      241 aggaacagaa acattataat gcactaattg ggttgctttt tgaataatgc aacagcctga
      301 ttatttaaca ggttagctat gaagtcgtta tgaagacatc tgataatgaa agtataaaat
      361 atgaaattac tggccaggcg gtgctccaga tactgcgcat gaagataaat ttttcgttgc
      421 agactcttat taagcaatta ctcgtaatga aatcggctga ggaaaatact ttccgacgag
      481 atttaattga cagcataatt cgtgatttta gcaacagtga tacaggaggg ccaaaccgaa
      541 gaacagcgat agctgacaat aaaagtatgt tcactggtaa gaaaataaac agaatacatt
      601 aaaatttcat aagtaagatg agaggttacc atgcttgaag atactactat tcataatgca
      661 atatctgata aagcgttatc aagttacttt cgtagttcag gtaatttgtt agaagaagag
      721 tctgctgtgt tagggcaggc tgtgaccaat ttaatgcttt caggcgataa tgttaataat
      781 aaaaatatta tcttaagtct gatacactcc ctggaaacaa caagtgatat tctcaaagct
      841 gatgtgatta gaaaaacact ggaaatcgtg ttgcgataca cagctga
//
LOCUS       DAZFRG010000571          881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFRG010000571 DAZFRG010000000
VERSION     DAZFRG010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.574"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..756
                     /locus_tag="ACHKUC_005242"
     CDS             <1..756
                     /locus_tag="ACHKUC_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621267.1"
                     /translation="EPGKGANRVDGGSGYDTLLLSDVMDDWNIYRVSDGKLFFHSERG
                     AGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEGTNGD
                     DSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDWLYGG
                     GGNDYLYGGTGNDVFILDSSCGGNTVIMDFNKNKGEMDKLFLSREIVSSADEFISCLK
                     QVNSNIDVNFGDINISLINTSLDNITHSCVIVV"
BASE COUNT          267 a          119 c          222 g          273 t
ORIGIN      
        1 gaaccaggta agggagcaaa cagggttgat ggtggcagtg ggtatgacac gctgcttctt
       61 agcgatgtaa tggatgactg gaatatatac agggtcagtg atgggaagct gtttttccac
      121 tcagaacggg gagccggtct gactgaggca acaggtattg aaggtatctc tttttctggt
      181 gactggagga gtaaactaag cccttatcag atagaagaag agcagattac agaccacagg
      241 tatcttctga agtggcgtaa taaaaatgtt gattacggaa aacatacaga aggtacaaac
      301 ggagatgata gtctgttcgg aaatattatt tttggtcttg acggaaatga tatattacat
      361 agtgccgggg gagaaggagt aagtctttta cacggaggtc gcgggaatga ttatcttcat
      421 ggggcttctg gtaatgatga gttgtacgga gcagaaggta acgactggtt atatggtggg
      481 ggaggtaatg attatctgta tggtggtacc ggtaatgatg tgtttatttt agactcatct
      541 tgtggcggaa atactgttat tatggatttc aataaaaaca aaggagagat ggacaaatta
      601 tttctttcaa gagaaattgt gtccagcgca gatgaattca tatcatgttt aaaacaagtt
      661 aattcaaata ttgatgttaa ttttggtgat attaatatat cgctaataaa tacatcactt
      721 gacaacatca ctcatagttg tgtcattgtt gtctgatttc cctgacattt caggcaggca
      781 gttgaattaa aatattttga agtggtcatt gaatatactg cctgtttttc tcgaatatga
      841 ggatatataa tggttattta tatcagcaca ctactttgtt g
//
LOCUS       DAZFRG010000572          853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFRG010000572 DAZFRG010000000
VERSION     DAZFRG010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.575"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            223..825
                     /gene="fimB"
                     /locus_tag="ACHKUC_005243"
     CDS             223..825
                     /gene="fimB"
                     /locus_tag="ACHKUC_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO6621268.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          249 a          160 c          192 g          252 t
ORIGIN      
        1 taaatatata aaggtacata gcatgcaaga gcatggcgtt tgtatggcaa cgttattata
       61 attaacagtt gctactccat ttaagttcac tcagaagaac tggtccactt acgttagtta
      121 ttaagcaaac gttcgctttt ataaacataa tcaggataaa aatgttggat tatttctaac
      181 ccagcacagc tagtgcgcgt ctgtaattat aagggaaaaa cgatgaagaa taaggctgat
      241 aacaaaaaaa ggaacttcct gacccatagt gaaatcgaat cactccttaa agcagcaaat
      301 accgggcctc atgcagcacg taattattgt ctgactttgc tttgttttat tcatggtttc
      361 cgggcgagtg aaatttgtcg attgaggatt tcggatattg atcttaaggc aaagtgtata
      421 tatatccatc gattaaaaaa aggcttttca acaacgcacc cgctattgaa taaagaagtt
      481 caggctttaa aaaactggtt gagtatccgt acttcgtacc cgcatgctga gagcgagtgg
      541 gtatttttat cacgtaaggg gaatccgctt tctcggcaac agttttacca tattatctcg
      601 acttccggtg gtaatgccgg gttgtcactg gagattcatc cgcacatgtt acgccattcg
      661 tgtggttttg ctttggcgaa tatgggaata gatacgcgac ttatccagga ttatcttggg
      721 catcgcaata ttcgtcatac tgtctggtat accgccagca atgcagggcg tttttacggc
      781 atctgggata gagccagagg acgacagcgt cacgctgttt tatagagata gttgagatac
      841 cagggatggt gtt
//
LOCUS       DAZFRG010000573          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFRG010000573 DAZFRG010000000
VERSION     DAZFRG010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.576"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..444
                     /locus_tag="ACHKUC_005244"
     CDS             <1..444
                     /locus_tag="ACHKUC_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFO6621269.1"
                     /translation="SFIQNTGIARILPLRNGRLITCLTDNQEPSPCLIWLPAPDITAT
                     FPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYYSGSWYLIALQKGKLQVFPLAD
                     IKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIHKLINTFNL"
     gene            592..>852
                     /locus_tag="ACHKUC_005245"
     CDS             592..>852
                     /locus_tag="ACHKUC_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621270.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTG"
BASE COUNT          213 a          239 c          192 g          208 t
ORIGIN      
        1 tcttttatac agaataccgg gatcgcacgg atacttccgc tccggaacgg acgactgata
       61 acctgtctta ccgacaacca ggagccctct ccctgcctta tctggctacc ggcgccggat
      121 atcactgcaa cgttccccga gtgtttctcg caactcatcc tggcaataag acagtgtatc
      181 cacatctctc tgatgactga gcgatggtat ccgtcactgg agccctgccg gctcatttat
      241 tacagcggta gctggtatct gatcgcgtta cagaagggaa aactgcaggt ctttcctctg
      301 gcagatatca aatcagtcag cctgacatca gaacggtttg aacggagagg ccacatccac
      361 agtctggtcg ctgaagagcg ttttatctcc gccctgccac atttctcttt catccataaa
      421 cttatcaaca cctttaacct gtgatcgccg gcctgccaaa gccgtcccga caggtatgga
      481 gacaatatgt tgaacagaaa actaaatata cggctacgtc attccctgaa cagtcactgc
      541 ataccttcca tcattatcaa taacaccgta cgttcatttc agaggtcagt catgaatacc
      601 agagctcttt ttcccctgct gttcactgtg gcatcattct ccgcctccgc cggcaactgg
      661 gctgtcaaaa acggctggtg tcagaccatg acggaagatg gtcaggcgct ggtaatgctg
      721 aaaaatggca cgattggtat taccggcctg atgcagggat gcccgaatgg tgtacagacg
      781 ctcctgggca gccgtatcag tattaacggt aacctgatcc ccacatcaca aatgtgtaat
      841 cagcagacgg ga
//
LOCUS       DAZFRG010000574          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFRG010000574 DAZFRG010000000
VERSION     DAZFRG010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.577"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>845
                     /locus_tag="ACHKUC_005246"
     CDS             <1..>845
                     /locus_tag="ACHKUC_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6621271.1"
                     /translation="YSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVAGTVN
                     AANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASG
                     AATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVG
                     ATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDL
                     LSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGD"
BASE COUNT          272 a          165 c          174 g          234 t
ORIGIN      
        1 atactccctt aaatatagtg ctgccactgg cggactcatt gctgtttctg aattagcgaa
       61 aagagtttct ggtaaaacaa accgaaaact tgtagcaaca atgttgtctc tggctgttgc
      121 cggtacagta aatgcagcaa atattgatat atcaaatgta tgggcgagag actatcttga
      181 tcttgcacaa aataaaggta ttttccagcc cggagcaaca gacgtaacaa tcactttaaa
      241 aaacggagat aaattctctt tccataatct ctcaattccg gatttttctg gtgcagcagc
      301 gagtggcgca gctaccgcaa taggaggttc ttatagtgtt actgttgcac ataacaaaaa
      361 gaaccctcag gccgcagaaa ctcaggttta cgctcagtct tcttacaagg ttgttgacag
      421 aagaaattcc aatgattttg agattcagag gttaaataaa tttgttgtgg aaacagtagg
      481 tgccaccccg gcagagacca accctacaac atattctgat gcattagaac gctacggtat
      541 agtcacttct gacggttcaa aaaaaatcat aggttttcgt gctggctctg gaggaacatc
      601 atttattaat ggtgaatcca aaatctcaac aaattcagca tatagccatg atctgttaag
      661 tgctagtcta tttgaggtca cccaatggga ctcatacggc atgatgattt ataaaaatga
      721 taaaacattt cgtaatcttg aaatattcgg agacagcggc tctggagcat acttatatga
      781 taacaaacta gaaaaatggg tattagtcgg aacaacccat ggtattgcca gcgttaatgg
      841 tgacc
//
LOCUS       DAZFRG010000575          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFRG010000575 DAZFRG010000000
VERSION     DAZFRG010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.578"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..429
                     /locus_tag="ACHKUC_005247"
     CDS             <1..429
                     /locus_tag="ACHKUC_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6621272.1"
                     /translation="VPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNG
                     QNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSK
                     RERYSEKWQRRTHSVWRHRHWHASAFTGICNDLAAASACM"
     gene            426..>843
                     /locus_tag="ACHKUC_005248"
     CDS             426..>843
                     /locus_tag="ACHKUC_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6621273.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGGSMALWGTALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGR"
BASE COUNT          163 a          234 c          270 g          176 t
ORIGIN      
        1 gtgccgcacc tgacgggacg gcgctttgag cacggggtga cggactgtta caccctgttc
       61 cgggatgcat accatctggc ggggactgaa atgccggatt ttcatcgcga ggatgactgg
      121 tggcgcaacg gtcagaacct ttacctggac aatatggcgg tcaccggctt ttaccgggtg
      181 cccctgtcct ctgcacaggc gggcgatatt ctgctgtgct gctttggtgc ttcggtaccg
      241 aaccatgccg ccatttactg cggcaacggt gagctgcttc accatctgcc tgaacaactg
      301 agtaaacggg agaggtattc cgaaaaatgg caacgacgaa cgcattctgt ctggcgtcac
      361 cgccactggc acgcatctgc cttcacgggg atttgcaacg atttggccgc cgcctcagcc
      421 tgtatgtgaa cacggcagcg gaagccattc gcgccctgtc gatgcagatg ccgggctttc
      481 gccgtcagat gaacgaaggc tggtaccaga tacgtattgc cggtgatgac acggcaccgg
      541 aggcggtgta tgcccgtctt cacgaacagc tgggtgaggg aacggtcatc cacattgtgc
      601 cgcgactggc cggggccgga aagggtggac tgcagattgt gctgggggcg gcagccatcg
      661 tgggctcttt cttcacggcc ggaggctcga tggcgttatg gggtacagcc ctgagtgccg
      721 gtggtttttc tgccaccacg atgctgtttt cactgggtgc cagcatgata ctgggcggag
      781 tggcccagat gctggccccg aaggcaaaaa caccggatta ccgcgcaacg gataacggca
      841 gac
//
LOCUS       DAZFRG010000576          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFRG010000576 DAZFRG010000000
VERSION     DAZFRG010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.579"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..156
                     /locus_tag="ACHKUC_005249"
     CDS             <1..156
                     /locus_tag="ACHKUC_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO6621274.1"
                     /translation="PFCTGKYRWHGSTEAYTGREVQDIPGVLAVFAERRKDSFGPYVR
                     LMSVTLN"
     gene            175..819
                     /locus_tag="ACHKUC_005250"
     CDS             175..819
                     /locus_tag="ACHKUC_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO6621275.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
BASE COUNT          189 a          235 c          242 g          176 t
ORIGIN      
        1 ccgttctgta ccggcaaata ccgctggcac ggcagcactg aagcgtatac cggtcgtgaa
       61 gtgcaggata ttcccggtgt gttggccgtg tttgctgaac gtcgtaagga cagttttggc
      121 ccgtatgtcc ggctgatgag cgtcaccctg aactgaatca ggacgggcat tcagatgagc
      181 agcattatca ccatcacagt gtatacgctg gatgaacttt cctgcccggc acggggaaaa
      241 gcccgcgact ggtaccgtca gcatcatgct gacagcaact ggtatgaaaa cgtgtatgaa
      301 gacttcaggg aggtctgtgg cattttcggg attgacctcc gccagcgcgt tttccggctc
      361 agcaatggca ggtttatgga agagccgtgc atctggttca gcggattctg cagccagggg
      421 gacggagcct gttttgaagg gcgctggcac tggcagccag cgacaccccg gaaaatcagg
      481 gaatatgccc ctcaggaccg tgagcttcat cgtatcgcgg ataccctgca ggcggtgcag
      541 aaacgtaact tctggcagct tcaggctgaa atcagtcacc ggggacgtta ctgccatccg
      601 tacagcatgg atatcaccgt cacccggaac agtccgactg gtcaggcaat gacaaccgat
      661 gcggaagccg ctgtcagcga agccctgcgc gacctggcct tctggctgta ccgccagctg
      721 gaaaacaagt acgactggct gacctcagac accgctgtgg atgaggccct tctcatcaac
      781 gaatatacct tcaccgaagc cggtctgcgc gccggctgac ccgtcatcgt taaactatca
      841 tt
//
LOCUS       DAZFRG010000577          835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFRG010000577 DAZFRG010000000
VERSION     DAZFRG010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.580"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..687
                     /locus_tag="ACHKUC_005251"
                     /pseudo
     CDS             20..687
                     /locus_tag="ACHKUC_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
     gene            complement(726..>835)
                     /locus_tag="ACHKUC_005252"
     CDS             complement(726..>835)
                     /locus_tag="ACHKUC_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6621276.1"
                     /translation="VDPESYLRYVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          215 a          168 c          237 g          215 t
ORIGIN      
        1 aataatggag ttaattgaaa tgaaaaaagt ttttgctgtt tcagcagtta cagctgcctg
       61 tatgcttgca gcaaatgcgg gtgctgagga aggtaaaagc ggattttatc tgaccggtaa
      121 agcaggtact tcagcagtat ctctttctga ccagagtttc ctgtccgaaa ataaagagga
      181 aacatcaaag tatagggggg ggatggccat aacacggtat tcagtggagg tatagcgttt
      241 ggttatgact tgcatccgca gttcagtatt cctgtgcgta cagaactgga gttttacgcc
      301 cgtggaaaag ctgacacaac atacaacctc tataagggcc acgcgggaca tagtgacttg
      361 aagaatgagg tgtcagtcaa cagcctgatg ctgaatgcgt actatgattt cagaaacgac
      421 agtgcattta cgccatgggt gtctgccgga attggttacg ccagaattca tcaggaatca
      481 acaggtagcg gtagttggga ttatggctac ggtgagcgag tgcgttattc tatatcacgc
      541 tcaggccctg ctgacaactt cgcatggagc cttggtgctg gtgtccggta tgatgtaacc
      601 cccgatatta ctctggatct cagttatcgc tatcttgatg cgggtgatgc cagtgtgagt
      661 tataaggacg agtggggcga taaatatagt aagcgtacag cgagggccgt attgacgggg
      721 atgtgttatt cagctggcag tgctatgcgc cacggaagca gttcgctgac ccggttgacc
      781 ggccagtctg ctatgacgcc aggcacatag cgaaggtagc tttctggatc cacgt
//
LOCUS       DAZFRG010000578          828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFRG010000578 DAZFRG010000000
VERSION     DAZFRG010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.581"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(528..>828)
                     /locus_tag="ACHKUC_005253"
     CDS             complement(528..>828)
                     /locus_tag="ACHKUC_005253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621277.1"
                     /translation="RLTYDIISQLHLPQNGYFMERSNFLVAIIMLILRSLAIIPADSS
                     AYAMRLLHSFLSWRYGGTQEMIFSSTFVGKYSFGHYDLNLPQSQPFAGRYGKQTK"
BASE COUNT          247 a          154 c          176 g          251 t
ORIGIN      
        1 ttacccagag tcataaaata gaatgtctgt tgatctcatt ttcatattcc tttcagactg
       61 aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat agattgcgag
      121 atgccctgtc tcattgacta tcttggtgca cctttatacc tgttatacca gatcaaaaat
      181 cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat gtcgatcttt
      241 ttctaagagg aagatgccat gtgaagccag acgaacactt gcggtggtct tcaaaaacta
      301 aagatcttag tttaactatt tgttttataa ataatttgtt tagagtctaa acaaggggag
      361 ctttgcaagc taactcagtg agcttggtga aaatcagtgt ttacccgcca tcaggctgag
      421 cataattctc atcatgaaat atgtttcctg gtttgtggct tgtaactggt cacttctgaa
      481 gtcgatctgg agaggcttgt tgatgttggt gttttcagga tgatgtttca cttagtttgt
      541 ttgccgtatc gcccggcgaa tggctgtgat tgaggaaggt ttaagtcgta gtgaccaaag
      601 ctatatttac caacgaatgt agatgaaaaa atcatctcct gcgttccccc atatctccag
      661 gataaaaagg aatgtaacaa tctcatggcg taagctgacg aatcagcagg aataatcgct
      721 agggacctaa gaattagcat gataatagcc actaagaaat tactgcgctc catgaaatag
      781 ccattttgtg gcaaatggag ttgactaata atgtcatatg tgagacgg
//
LOCUS       DAZFRG010000579          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFRG010000579 DAZFRG010000000
VERSION     DAZFRG010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.582"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..751
                     /locus_tag="ACHKUC_005254"
     CDS             <1..751
                     /locus_tag="ACHKUC_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621278.1"
                     /translation="FSLLAGCASESPIDEKKKKTQTTQISINKNVPQQLTDKDLYGNE
                     TTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMRKYYTVATFSGIP
                     DRKKTVTCNKDKNKDESLDIVDAENMNWMQALRFVAAKGHQKAIIVFQDTLQTGKYDS
                     GLKSIVWTDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYESKVIFPQIGNKN
                     SDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
BASE COUNT          266 a          143 c          181 g          224 t
ORIGIN      
        1 attctcgctg cttgcaggat gtgcctctga atctcctatt gatgaaaaaa agaaaaaaac
       61 tcaaaccaca caaatttcta ttaataaaaa cgttccccag caactaacag acaaagattt
      121 atatggtaat gaaaccacac tggctgtatc cgaggaggat attcaagctg cgcttgaagg
      181 agatgagttt cgtgtgcctc ttaattctcc tgtaatactt gttcagtctg gcagtcgcgc
      241 accggaaacc attatgcagg aggagatgag aaaatattat acagttgcca cattttctgg
      301 cattcctgac aggaaaaaaa ctgtaacctg taataaagac aaaaacaaag atgaaagcct
      361 ggatattgta gatgccgaaa atatgaactg gatgcaggca ttgcgttttg ttgcagcaaa
      421 aggccatcag aaagcgatta ttgttttcca ggatacgttg cagacaggaa aatatgactc
      481 tggattgaaa tcaatagttt ggacagatta taaaaatcaa aaattgactg acacgatgtc
      541 actgcgttat ttggttcgat tcacgttggt ggatgtggct acaggtgagt gggctacctg
      601 gtcgcctgtg aattatgaat cgaaagtgat ttttccgcag atagggaata aaaattctga
      661 cagccttgat gtggcagaac agcagatttc acaacttaag cagaaaacgt atgcagcagt
      721 ggttaaagac ctggtgaatc gatatcagta gtgaggtttt ataccagacg ttttagtggt
      781 acttagcaag aaagagcatt tctcttttct attt
//
LOCUS       DAZFRG010000580          812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFRG010000580 DAZFRG010000000
VERSION     DAZFRG010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.583"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..>812)
                     /locus_tag="ACHKUC_005255"
     CDS             complement(41..>812)
                     /locus_tag="ACHKUC_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6621279.1"
                     /translation="SFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDHNCDGRRTV
                     EIHKLDLSDWQAFNKLATRCNAYDGITMNGDNSFGWNHEATLDNIHAQKYNKAYTGAR
                     LTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSS
                     ELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSSAAIYSGLG
                     FERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT          217 a          162 c          180 g          253 t
ORIGIN      
        1 accattggtc ggcgttttgc tttctatttt tcgtcaggta ttagtttcgc aagtagatcc
       61 attcaccatt ctcttgtgtc cataagtcag gatggtttgc tggcgttaga tgcatacgat
      121 cactccttat gtctcggtcc tgcggcactc gctcgaaacc caaacccgag tagattgctg
      181 ctgaacttga taaggaacgc agaccaattt tcaaatcatg gggtaaatca gaatacgcgt
      241 ttagcaatgc agcaatgatg aatccgaggg cattagaaac gcctgtaaca gtgcgaagtt
      301 ggtcgatata aagatcgatt gttggtagtt cagatgacca gcaattaggc atttgggcag
      361 gaagggttac aacgtatcct atcgtttggc cggtatagtc tggagtacca taaccaggtc
      421 ccaaaaccac ttcgtgtatt gtgtatttac tattgggttc gaaggattcg acatcttgca
      481 ataaatattt cagttctgcg gttaacctcg cacccgtgta agctttattg tatttctggg
      541 cgtgaatatt atctaatgta gcttcatggt tccatccaaa agaattatca ccattcattg
      601 ttattccatc gtaagcatta cacctcgtcg ctaacttatt gaatgcttgc cagtctgaga
      661 gatctagttt gtggatctca acagtacgtc tgccatcgca attatgatct cttccacgat
      721 ttgaaagtaa tgtagcgcgt gcgtattgat gaaattttaa ttttactggt ttgtcggtgg
      781 acgaaaaacg agataaatat gcaggaaatg aa
//
LOCUS       DAZFRG010000581          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFRG010000581 DAZFRG010000000
VERSION     DAZFRG010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.584"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>791
                     /locus_tag="ACHKUC_005256"
     CDS             <1..>791
                     /locus_tag="ACHKUC_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO6621280.1"
                     /translation="SQTEISTANSSTVNASYVEPSAEKIISPSDKLNNLFERNMSQPY
                     ILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRGKYLLQAIRDVTPLPVTALVY
                     SHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQFMNSRLPVATQVLSKKDDQF
                     KFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLLNPDQLPMMGFAVSDTLVYHD
                     SNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLRDTTIKVR"
BASE COUNT          241 a          150 c          166 g          234 t
ORIGIN      
        1 tttctcaaac ggaaatttcg actgcaaatt cgtctacagt taatgcaagt tatgttgaac
       61 cttcagcgga aaaaataata tcgccaagcg ataaactcaa taaccttttt gaaagaaata
      121 tgtctcagcc ttatatttta caaaaaattg gcgagagaac ttattatgtt caacgctatt
      181 tttattccac tacgttttat gtcggagaca aaggggtttt gctgtttgat gctccggagg
      241 gtcgcggtaa atatttatta caggcaattc gtgatgtgac tccattaccc gtaacagcgc
      301 tggtttattc tcattaccat gtggatcaca ttggcgatag tccattttgg aacgatgagg
      361 cgaagaaaga aggcgtgaat ctgcgtatta ttgccagtaa agccactgct gaaaaaatgc
      421 aatttatgaa cagtcgctta cccgtcgcca ctcaggtttt aagtaaaaaa gatgaccagt
      481 ttaaatttga aaagcagacc attgaattac accgatttgt taaagcagga cataccgacg
      541 accattctgt atggttgcta aaacaggaaa aagtagctca cagcccggat ctcctgaatc
      601 ctgaccaact gccgatgatg ggcttcgccg tatcggatac gttggtttac catgacagta
      661 atttgcgtca ggttgaaatg cttgactgga aatattttat tggcgggcat ggtaatattg
      721 gttcacacga tgattttaaa tttcaacgcc agtttcttaa tgatcttcgg gatacgacaa
      781 ttaaagtcag a
//
LOCUS       DAZFRG010000582          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFRG010000582 DAZFRG010000000
VERSION     DAZFRG010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.585"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..>788
                     /locus_tag="ACHKUC_005257"
     CDS             44..>788
                     /locus_tag="ACHKUC_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HFO6621281.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLIT"
BASE COUNT          251 a          144 c          174 g          219 t
ORIGIN      
        1 taaataattc aacaaatgta acacacccta tccaggaggg atgatgaaaa ctgtatttac
       61 aacattatca gtcatattcg gcgtgatatt ttctcacagt gttctagctc aggacgttac
      121 tatttattat acgaatgaca ttcacgcaca tgtcaatccg gcaaaaatca tcgctgttga
      181 taagaacaga cttataggtg gtatggctaa tattgcaggc atcgttaatg aagcgaagaa
      241 aaaaagcaaa gatgtgtttt tctttgatgc aggcgactat tttaccgggc cgtatatcag
      301 cacactgacc aaaggtgaag ctattattga tatcatgaac actatgccct ttgatgcggt
      361 gtctgttggt aatcacgagt tcgatcacgg tgtgcccaat atggtgtctc agttatcaaa
      421 agcaaaattc cccattctgc tgggaaatat ctactacacc gatacgaata aaccagtttg
      481 ggatcatccg tggaccatta tcgaaaaaga tggtttaaag attggtgtga ttggattgca
      541 tggcgcattc gctttctatg atacagtcgc ggcaaaagca cgtgaaggcg tagaagccag
      601 agacgaaatt aaatatttaa ataaagcact tgcagaatta aaaggtaaag tcgacattac
      661 cgttttgcta attcacgaag gtgtcccggc gcgtcaatca agctttggta gtaaagatgt
      721 ggagcggcta ctacaggcag atattgagac agctaaaaaa gtgaatggtg ttgatgtctt
      781 aattaccg
//
LOCUS       DAZFRG010000583          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFRG010000583 DAZFRG010000000
VERSION     DAZFRG010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.586"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..135
                     /gene="ymgI"
                     /locus_tag="ACHKUC_005258"
     CDS             <1..135
                     /gene="ymgI"
                     /locus_tag="ACHKUC_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO6621282.1"
                     /translation="KNIIPIITVTLILNYLNNSERSLVKQILIEDEIIVCATYLIPDN
                     "
     gene            137..481
                     /gene="ymgG"
                     /locus_tag="ACHKUC_005259"
     CDS             137..481
                     /gene="ymgG"
                     /locus_tag="ACHKUC_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO6621283.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            485..>786
                     /locus_tag="ACHKUC_005260"
     CDS             485..>786
                     /locus_tag="ACHKUC_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO6621284.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITA"
BASE COUNT          227 a          150 c          210 g          199 t
ORIGIN      
        1 aaaaacataa ttcctatcat cacagtcacg ctcattttga attatctgaa taatagcgag
       61 cgttctttag ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta
      121 ataccggaca actaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt
      181 ctgctccacg ccagcgatgg cggatatgaa tcgcaccacg aaaggtgcat tgctaggcgc
      241 aggcgttggt ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg
      301 cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc
      361 aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg
      421 ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata
      481 aggaatgatg atgaaaaaat ttgctttact ggcgggtctg tttgttttcg caccaatgac
      541 ctgggcgcag gactacaata ttaagaacgg tttgccatca gaaacctata ttacttgtgc
      601 agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtaat
      661 gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc
      721 caaagtggta gaaaaactga atcaggtttg cgctaaagat ccgcaaatgc tgttgatcac
      781 ggcaat
//
LOCUS       DAZFRG010000584          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFRG010000584 DAZFRG010000000
VERSION     DAZFRG010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.587"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(299..>775)
                     /locus_tag="ACHKUC_005261"
     CDS             complement(299..>775)
                     /locus_tag="ACHKUC_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6621285.1"
                     /translation="CLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHV
                     NCLLLSDSKQSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSI
                     LDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          227 a          164 c          139 g          245 t
ORIGIN      
        1 aacaacgata tccgtaccta accggatatc taatgtcata agcatccctt taattatccc
       61 ctcaacctct tgtaactttc ccactcttga actccacata gtccaaacgc cggatgcaaa
      121 aaaatcacac gtgcatttct gaaattgtga tgaaggaaga tagtatcaat tatatgtgct
      181 tataaacgtc cccgattagc gcagaagtca ccggattgtt caggctccaa taggttgagt
      241 atgccgtatt tattttccaa aataaaaatc gatgctgttg ctccttcata aaatactttt
      301 actctctgga cgcttttctt agctctcttg gtgttatctt aaaacgatta tggaaccttt
      361 cagtaaaacg agaaggacac ttgtaaccat tttccctggc aatctcgctg atgggtttta
      421 ctgtcgtttg tatagcagac aacgcattat ttaacctcac gtcgtccaat atactttgaa
      481 aacttattcc ctcgcttgcc agacggcgat gcaatgtgga aacagaaatg tagagatatc
      541 tcgctacctt gtttgctgtc cattttgttc caggttcgga tagcattagg ttataacaac
      601 gacttattaa tgattgttta ctatctgata aaagtaaaca attaacatga tgtgctccta
      661 atgaaagtag aacgcccatt gctaagtgtt cctgaatttg cgttgagaag cctcggaaaa
      721 tagatgtttt tagttgctcc caacaatata ttaactcggg gttctgaggt aagca
//
LOCUS       DAZFRG010000585          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFRG010000585 DAZFRG010000000
VERSION     DAZFRG010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.588"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..733
                     /gene="fimE"
                     /locus_tag="ACHKUC_005262"
     CDS             137..733
                     /gene="fimE"
                     /locus_tag="ACHKUC_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO6621286.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          212 a          145 c          185 g          232 t
ORIGIN      
        1 tgatatttaa tgtaattgac tgatagccac atcactccgt gtgtggttat ctttttatct
       61 attgggctaa ttttgaccga ttgaggtttc ctataggtat tcattcaaat atatctcagt
      121 taggagtact actattgtga gtaaacgtcg ttatcttacc ggtaaagaag ttcaggccat
      181 gatgcaggcg gtttgttacg gggcaacggg agccagagat tattgtctta ttctgttggc
      241 atatcggcat gggatgcgta ttagtgaact gcttgatctg cattatcagg accttgacct
      301 taatgaaggt agaataaata ttcgccgact gaagaacgga ttttctaccg ttcacccgtt
      361 acgttttgat gagcgtgaag ccgtggaacg ctggacccag gaacgtgcta actggaaagg
      421 cgctgaccgg actgacgcta tatttatttc tcgccgcggg agtcggcttt ctcgccagca
      481 ggcctatcgc attattcgcg atgccggtat tgaagctgga accgtaacgc agactcatcc
      541 tcatatgtta aggcatgctt gcggttatga attggcggag cgtggtgcag atactcgttt
      601 aattcaggat tatctcgggc atcgaaatat tcgccatact gtgcgttata ccgccagtaa
      661 tgctgctcgt tttgccggat tatgggaaag aaataatctc ataaacgaaa aattaaaaag
      721 agaagaggtt tgatttaact tattgataat aaagttaaaa aaacaaataa atac
//
LOCUS       DAZFRG010000586          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFRG010000586 DAZFRG010000000
VERSION     DAZFRG010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.589"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..182
                     /locus_tag="ACHKUC_005263"
     CDS             <1..182
                     /locus_tag="ACHKUC_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621287.1"
                     /translation="YCTQHSVQELSFSLFYSSSGGQFSESVTPGKLFMFIPGRKPVPT
                     IPWMMLFAGTLLCEN"
     gene            377..769
                     /locus_tag="ACHKUC_005264"
                     /pseudo
     CDS             377..769
                     /locus_tag="ACHKUC_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          198 a          163 c          173 g          235 t
ORIGIN      
        1 aatattgtac tcagcattca gtgcaggaac tgtcattttc tttattttat tcttcttcag
       61 gagggcagtt ttctgaaagc gttactccgg ggaaactgtt tatgtttatt cccggcagga
      121 aacccgtacc tacaataccc tggatgatgc tttttgctgg tacgttgttg tgtgaaaatt
      181 aatcaacatg tgtgctccgg agagcgcccg tagacgcaca gattcattct gacattcttt
      241 agcgacaaaa taccgggtgg ttattattta acctgaaaat cgacagaaat ttttatcctg
      301 tattgattct gatgctattt ttgctattag tagcaatagt tgcggtactt tatgttctgt
      361 gtgttgtgag gtcattatgc catactttca gtatcctgat gagtttcccc tgagctcgct
      421 gcctccactc attcgtgatg ccgttattga agctcagcaa atcacgcagg ctcccctggg
      481 gcttgtggca gcatctgccc tgggcgctgt ttcacttgtc tgccagaatc tgattgatgt
      541 ctgcagactg aatacactgc ggggccggtt tctcttttct tactgacgct tgctgagtcc
      601 ggagaacgaa aaaccgcagt ggacaaatta ttaatggagc ccctgtacca gcaggagatg
      661 ctgttatatt ccagacacaa aaatgaactg acgacatgga aaaataagga agagctttta
      721 aaggcgcaga aaaaagcatt gttgtcaaaa ctgaataaag aactccgca
//
LOCUS       DAZFRG010000587          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFRG010000587 DAZFRG010000000
VERSION     DAZFRG010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.590"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            434..679
                     /locus_tag="ACHKUC_005265"
     CDS             434..679
                     /locus_tag="ACHKUC_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621288.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHGYDADYICGCGEIMCPGCGHDL"
BASE COUNT          252 a          138 c          147 g          223 t
ORIGIN      
        1 gggacagttt tttataactc attaatccct acctgttgct ttattttcat tatgaaaagt
       61 taccaaatct tagcacctca gtcaggagag tcaggtcatc gataaagaat attccagtag
      121 agtggcgctc atgatcaaag ataacagcaa agttgatgac agtgctgacc gactgtcatt
      181 tacagaaacc ctgtttgtag caaattccat cttacctgac ctcacccaga caaagacaaa
      241 agagaatgat tgttatttac ttttcaaaaa aagtgtcata ttgtccatat aaagtcaaga
      301 ggtattttac gttatgtcac tgatgcaatt gttagtcagg atctcatggt tgtcgctcat
      361 gtataacctg agacttgaac gcacactaat agcgctgacg gtcatacatc gggaaatgtg
      421 aggaggcata agaatgaatt gtgataataa tcacagaaat gaagaattca ttgttacctt
      481 tgataaaggc aacaagcaag acaattcaag acgaaaacac gataattttc ctatagaggt
      541 agaatcctcc gtagagcagg agacacactg tatcacaaat aataagtcag cttccggtat
      601 agtaacacat ggctatgatg ccgattatat ttgtggttgt ggtgaaatta tgtgtcctgg
      661 ttgcggtcat gacctataac aaacattttt gcaaactgat acctgatcag gtacaagcct
      721 gttgattatt atgctgaata tttcatgtta taaaaccatc
//
LOCUS       DAZFRG010000588          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFRG010000588 DAZFRG010000000
VERSION     DAZFRG010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.591"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHKUC_005266"
     CDS             complement(<1..296)
                     /locus_tag="ACHKUC_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6621289.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEDFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDV"
     gene            complement(448..>757)
                     /locus_tag="ACHKUC_005267"
     CDS             complement(448..>757)
                     /locus_tag="ACHKUC_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6621290.1"
                     /translation="SVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKT
                     HDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDAFIVGIGYRF
                     "
BASE COUNT          187 a          223 c          171 g          176 t
ORIGIN      
        1 atgacatcat cttccgtcat ggcgccgaga aaatcattca gcgtacctgg tctggaacct
       61 tcatagacgg taatggtccc ggcatgtgaa ggcggaaaat cttcaaccag cagggtgacg
      121 ctgtactggc catactcaac atccatgctg taacgcccgg cttcatccgg attttcagag
      181 gccaccgtgt tcaccaccac cgtgctgctg gtccgtctgg ccttcagcac aatggtgcag
      241 ttctgtactg gttttcctgt gccatcttta agcacgccag aaatttttac tgtcatactt
      301 ttccaccaat aaaaaaagcc cgcagcagtg acgccacggg cttcaggaca gtgtaacttt
      361 acgtttcctc aaacgcagtt caccccataa ggcggatgaa cctgcgtatc ataacaatat
      421 ttacagaaga taaatcggcg tctgttgtca gaaacggtat ccgataccaa caataaatgc
      481 atccgttcgc cagtcgccac tgccggagcc ttcataagca atatcaatgg ccacggattc
      541 ggtcgggtta aactgcacgc cagctcccca cgccagagag gtattgctgt ggcgaccgtc
      601 atcacttccg gtcagcacgt cgtgcgtttt ccccttgttg tcagttacgc ggagataatc
      661 cccggagaaa gtcgacacac ggctgtaagc cacacctgcc atcgcataag cactgaacca
      721 ttcattcacg cgcacagacg gccccgccat tacgctg
//
LOCUS       DAZFRG010000589          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFRG010000589 DAZFRG010000000
VERSION     DAZFRG010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.592"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..530
                     /locus_tag="ACHKUC_005268"
                     /pseudo
     CDS             1..530
                     /locus_tag="ACHKUC_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          226 a          149 c          194 g          181 t
ORIGIN      
        1 gagcatgact gttacgcaat tagtgacaag agtcacggca gagaagaaat ccgtcttcat
       61 attgtttgcg atgttcctga tgagcttatt gaattcacgt ttgaatggaa agggctgaaa
      121 aaatatgcct ggcagtctcc tttcggtcaa taatagcaga acaaaagcaa gagtcagaaa
      181 taatggccag atattatatc agttctgctg atttaaccgc agagaagttc gtcacagcga
      241 tccgaaatca ctggcacgtg gagaataagc tgcactggca tctggacgtg gtaatgaatg
      301 aagacgactg caaggtaaga agaggaaacg cagcgggatt attttcaggg atacggcata
      361 tcgctattaa tattttgacg agagataagg tattcaaggc agggttaaga cgtaagatga
      421 gaaaagcagc aatggacaga aactatctgg cgtcagttca tgcggggagc tggctttcgt
      481 tatcttgccc tgacggtccc ggtataaaat acctgacctt cccctattag cggttacccg
      541 cgcctcacca gacccggtga catccctccc ggagcatagt cgacagggag aactaatcat
      601 gccgggtctg gctggccaac atcccattat aaggggtaca aataaggtaa cgggggtatt
      661 tcatcgaggc ggaaataaga ggaatatcgg tggagcagag tcttgccgac acatagtcga
      721 ttttatctgt tcattagaca tgaataattt
//
LOCUS       DAZFRG010000590          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFRG010000590 DAZFRG010000000
VERSION     DAZFRG010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.593"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..509)
                     /gene="clbA"
                     /locus_tag="ACHKUC_005269"
     CDS             complement(<1..509)
                     /gene="clbA"
                     /locus_tag="ACHKUC_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis phosphopantetheinyl
                     transferase ClbA"
                     /protein_id="HFO6621291.1"
                     /translation="MRIDILIGHTSFFHQTSRDNFLHYLNEEEIKRYDQFHFVSDKEL
                     YILSRILLKTALKRYQPDVSLQSWQFSTCKYGKPFIVFPQLAKKIFFNLSHTIDTVAV
                     AISSHCELGVDIEQIRDLDNSYLNISQHFFTPQEATNIVSLPRYEGQLLFWKMWTLKE
                     AYIKYRGKGL"
     gene            complement(510..722)
                     /gene="clbR"
                     /locus_tag="ACHKUC_005270"
     CDS             complement(510..722)
                     /gene="clbR"
                     /locus_tag="ACHKUC_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis LuxR family
                     transcriptional regulator ClbR"
                     /protein_id="HFO6621292.1"
                     /translation="MDKFKEKNPLSLRERQVLRMLAQGDEYSQISHNLNISINTVKFH
                     VKNIKHKIQARNTNHAIHIANRNEII"
BASE COUNT          221 a          124 c          133 g          263 t
ORIGIN      
        1 aggcctttac ctcgatattt gatgtaagct tctttgagcg tccacatttt ccaaaaaagt
       61 aattgacctt cataacgagg aagtgaaact atgttagtag cttcctgtgg agtaaaaaaa
      121 tgctgactga tattcagata agagttgtct aaatctctta tttgttcaat atcgacacca
      181 agctcgcagt gagaactaat agcaacggct actgtatcta tagtatggga aaggttaaaa
      241 aaaatctttt ttgccaactg aggaaaaact ataaatggtt tgccatattt gcacgtacta
      301 aattgccatg attgtaatga gacatcaggt tgatatcttt ttagtgctgt tttgagcagg
      361 atacggctta aaatatagag ttctttatca ctcacaaaat gaaactgatc atagcgtttt
      421 atttcttcct cattgagata gtgaaggaag ttatctctac tggtttgatg aaaaaaacta
      481 gtatgtccaa ttaatatatc aatcctcatt tagataatct cattcctgtt agcaatgtgt
      541 atagcgtgat tcgtattccg agcttgtatt ttatgtttga tgtttttcac atgaaacttt
      601 actgtgttta ttgatatgtt aagattatgt gatatttgag agtactcatc accttgtgcc
      661 agcatgcgca atacttgtct ttcacgcaga gataacgggt ttttttcttt gaacttatcc
      721 atgtttcccc ccatcctgaa t
//
LOCUS       DAZFRG010000591          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFRG010000591 DAZFRG010000000
VERSION     DAZFRG010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.594"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..240)
                     /locus_tag="ACHKUC_005271"
     CDS             complement(<1..240)
                     /locus_tag="ACHKUC_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFO6621293.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVVSELARTRGKRGGVAVALSLAA
                     VTSLPVLSADIVVHPGETVNGGTLVNHDNQFVSGTA"
     gene            complement(573..>738)
                     /locus_tag="ACHKUC_005272"
     CDS             complement(573..>738)
                     /locus_tag="ACHKUC_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6621294.1"
                     /translation="SVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSV
                     ARAVWNWIFF"
BASE COUNT          199 a          183 c          192 g          164 t
ORIGIN      
        1 agctgttccg gatacaaact ggttgtcatg gtttaccagt gttccgccat tcactgtttc
       61 acccgggtgc acaacgatgt cagcagacag caccgggagt gacgtgaccg cggcaagaga
      121 cagtgcaacc gccacaccgc cacgtttacc ccgtgtgcgg gccagttcgg agacaaccac
      181 gaaagcgccc gtaatgtgat tccataccag cctgtagcag gtattcagat gtcgtttcat
      241 cagcatttcc ttaatcagag tgagagtgaa taaaaaagcc ggtatccaca gatgtggata
      301 ccggcaggta aagggttaca gtgccagagg tccggctgac ggaagtttct gacgagtggc
      361 tctgttgttc cctgaatgtc tttctgtacg tatctgtgca tgttactgac aaaggttatt
      421 attagtttat cgatcgatat aaacgatcaa taaaataggt atcgatcgtt ttattctatt
      481 attgagctat tgcacggaca agagagacag taacgaaaac agcgcttttg tgagcagact
      541 cagaggcagg gaggacatca tccacaacag gttcagaaga agatccagtt ccataccgcg
      601 cgggcaacag agaccactgt gtcacggacg gcacgcagaa ccgtgcgtgc aacagaggca
      661 acacagacgc tctccgctgt gtcaaatatc cggtccaccg caccggtaaa ctgctcacgg
      721 gcctgagcgc ggacagat
//
LOCUS       DAZFRG010000592          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFRG010000592 DAZFRG010000000
VERSION     DAZFRG010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.595"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..>738
                     /locus_tag="ACHKUC_005273"
     CDS             41..>738
                     /locus_tag="ACHKUC_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6621295.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVY"
BASE COUNT          246 a          124 c          163 g          205 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa gccaaaggcg gtggagatga aacgttcgta cagggacgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat
      481 ttttgaaatt ttgcttgaag ctgaagttga tgttcgtgat gtaactgaag aagaaggtaa
      541 tattgttatt tatactgaac ctactgacct tcataaagga atcgcggcgc taaaagcagc
      601 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
      721 tgtacaaaaa gtttatca
//
LOCUS       DAZFRG010000593          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFRG010000593 DAZFRG010000000
VERSION     DAZFRG010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.596"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>731
                     /locus_tag="ACHKUC_005274"
     CDS             <1..>731
                     /locus_tag="ACHKUC_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6621296.1"
                     /translation="ADGDYIEYRGISSNNRVTLDPLRLSNKELRWLASKKNLVIAVHK
                     SQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLREYADHQKAMDALEDGEVDIVL
                     SHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLTSSKPVNIARVANYPPDEVIH
                     QSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSSMISRYFTHSLNVVKYYNSPR
                     QYNFLLTRKDSIVLNEVLNRFVDALT"
BASE COUNT          230 a          160 c          126 g          215 t
ORIGIN      
        1 gcagacggag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
       61 ccactacgtc tgagcaacaa ggaattacgt tggctcgcga gcaaaaaaaa tcttgtgatt
      121 gcagtacaca agtcccaaac agctacgttg ttgcataccg attcgcagca acggattcgt
      181 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
      241 cgggaatacg cagatcatca aaaagcaatg gacgcgctgg aagacggtga agttgatata
      301 gtgttatcac atttagttgc ttcgccgcct cttaatgatg acattgctgc aactaatcct
      361 ctgataatta cctttccggc gctggtcact acccttcacg attcaatgcg accgcttacc
      421 tcatcaaaac cagtaaatat tgctcgagta gcaaattatc ccccagacga ggtaattcat
      481 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
      541 gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
      601 tcccgttatt ttactcactc cttaaatgta gtgaagtatt ataactcacc gcgtcaatat
      661 aactttttat taaccagaaa agattcaatc gttcttaatg aagtactcaa tcgatttgtt
      721 gatgctttaa c
//
LOCUS       DAZFRG010000594          721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFRG010000594 DAZFRG010000000
VERSION     DAZFRG010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.597"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..574
                     /gene="ybcL"
                     /locus_tag="ACHKUC_005275"
     CDS             23..574
                     /gene="ybcL"
                     /locus_tag="ACHKUC_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO6621297.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            584..>721
                     /locus_tag="ACHKUC_005276"
     CDS             584..>721
                     /locus_tag="ACHKUC_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6621298.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ET"
BASE COUNT          222 a          133 c          168 g          198 t
ORIGIN      
        1 aggtcacaac aatgaggttt ttatgaaaaa acttatcgtt tcaagtgtat tggcattcat
       61 aacattttct gcgcaggccg cagcatttca ggtcactagt aatgaaataa aaacaggaga
      121 gcaacttaca acgtctcatg tattttctgg atttgggtgt gaagggggta atacatcgcc
      181 atcattaacc tggtctggtg ctcctgaagg caccaaaagc tttgccgtaa ctgtgtatga
      241 tccagatgct cctacaggca gtggatggtg gcactggact gttgctaata ttccagcaac
      301 tgtaacatat ttgcccactg atgcaggaag acgtgatggt acaaaacttc cgactggtgc
      361 tgttcaaggc cgaaatgatt ttggttatgc aggatttggt ggagcatgtc ctcccaaagg
      421 agataaaccc catcattacc agtttaaagt atgggctcta aaaactgata agattcctgt
      481 ggattctaat tccagcggag cgttagttgg ttatatgctt aatgccaata aaattgcaac
      541 cgctgagata acaccagttt atgagataaa gtagggtaag agtatgctgg caaaagataa
      601 gtctaaccta aagattgaag aaatacggat gcataaacat catgagattc acagggttaa
      661 gcctcttatg ccagctttat gtcgtatccg tcagggaaaa aaagttatca attgggagac
      721 g
//
LOCUS       DAZFRG010000595          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFRG010000595 DAZFRG010000000
VERSION     DAZFRG010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.598"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>719)
                     /gene="kfiD"
                     /locus_tag="ACHKUC_005277"
     CDS             complement(<1..>719)
                     /gene="kfiD"
                     /locus_tag="ACHKUC_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis UDP-glucose
                     dehydrogenase KfiD"
                     /protein_id="HFO6621299.1"
                     /translation="LREGRALYDNLHPSRIIIGECSERAERLAVLFQEGAIKQNIPVL
                     FTDSTEAEAIKLFSNTYLAMRVAFFNELDSYAESFGLNTRQIIDGVCLDPRIGNYYNN
                     PSFGYGGYCLPKDTKQLLANYQSVPNKLISAIVDANRTRKDFITNVILKHRPQVVGVY
                     RLIMKSGSDNFRDSSILGIIKRIKKKGVKVIIYEPLISGDTFFNSPLERELAIFKGKA
                     DIIITNRMSEELNDVVDKVYS"
BASE COUNT          240 a          153 c          112 g          214 t
ORIGIN      
        1 actatagact ttgtcgacca catcgttcaa ctcctctgac attcggttag tgataataat
       61 atcagctttc cctttaaaga tcgccagctc ccgttccaaa ggtgagttaa agaatgtatc
      121 tccagaaata agcggctcat aaataattac tttcacgcct tttttcttga tacgctttat
      181 aataccaaga atagaagaat ctctaaaatt atctgaacca cttttcataa tcaaacgata
      241 aacccccaca acttgtggtc tatgtttcaa aataacatta gtgataaagt ccttacgtgt
      301 acggttagca tcaacaattg cagatataag tttattcgga acagactgat agttggctaa
      361 taattgcttg gtatcttttg gcaaacagta gccaccataa ccaaaagaag gattattgta
      421 gtaattacca atgcgcggat ccaaacaaac accgtcaata atctgacgcg tattcagacc
      481 aaaactttct gcgtaactat ccaattcatt aaaaaatgca actcgcatag ccaaataagt
      541 atttgaaaat aacttaatcg cttccgcttc cgtagaatct gtaaataaaa cgggtatatt
      601 ttgtttaatc gctccttcct gaaataacac tgccaaacgt tctgcccgtt cagaacattc
      661 accgataata atgcgagatg gatggagatt atcgtatagg gctcttcctt ctcgtaaaa
//
LOCUS       DAZFRG010000596          711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFRG010000596 DAZFRG010000000
VERSION     DAZFRG010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.599"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>711)
                     /locus_tag="ACHKUC_005278"
     CDS             complement(<1..>711)
                     /locus_tag="ACHKUC_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO6621300.1"
                     /translation="FSLNMENARQMIKTCSEFSRGDMKVYAGASRMDVNESISLANYA
                     WEQGLDGVMIISPYYFPLDDEAIFDFYSTIASQTPAHIFIYNFPERTGYSVSPEICLR
                     LADKYPNIIGMKDTVPDAFHTAQVINTVKKNIPHFEVYAGYDNNFAHNILSGGDGCIG
                     GLSNIVPEFFASWMQAFTNDDLLAVAQHQQIVDQLMAVYSVNSPFIPTFKKALQLKGV
                     IQSERCSSPFNILDDIQSR"
BASE COUNT          220 a          144 c          150 g          197 t
ORIGIN      
        1 cgggattgaa tatcatcaag tatattaaat ggtgaagaac accgctctga ttgaataacc
       61 ccctttaact gaagggcttt tttgaaagtg ggaataaaag gagaattcac gctgtaaacg
      121 gccattagtt gatcgactat ctgctgatgc tgcgcgactg caagtaaatc gtcattcgtg
      181 aatgcttgca tccaggaggc aaaaaattca gggacaatat tggataaacc accaatacag
      241 ccatctccac cagatagtat gttgtgggca aaattattgt catatcctgc atacacttca
      301 aaatgaggga tatttttctt tacggtattg ataacctgcg cagtatggaa agcatcaggg
      361 acggtatctt tcataccaat aatattggga tatttatctg ccagtcgtaa acaaatctct
      421 ggtgatacgc tgtacccggt tctctccggg aagttataaa taaaaatatg agccggggtt
      481 tggcttgcaa tcgtactgta gaaatcaaaa atagcttcat catccagagg gaaataataa
      541 ggactgatga tcatcacacc atccaatcct tgctcccagg cgtaattggc cagtgatatt
      601 gattcattga cgtccatgcg actggctccg gcataaacct tcatatcgcc tcgggaaaac
      661 tcactacagg ttttaatcat ttggcgggcg ttttccatat taagagaaaa a
//
LOCUS       DAZFRG010000597          711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFRG010000597 DAZFRG010000000
VERSION     DAZFRG010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.600"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..711
                     /locus_tag="ACHKUC_005279"
                     /pseudo
     CDS             1..711
                     /locus_tag="ACHKUC_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          217 a          172 c          110 g          212 t
ORIGIN      
        1 gtctcctctt agaatgcaca tcgatagcga catactaacg ccttcatcag agcctattaa
       61 tcaatttgcg cgccagctca tcaccctact tgatacctct tatttaagtt cgatgctgtc
      121 atactgtgtt actcaggaat ttaccgcaag ctgtcgaaaa atttcacaaa attgttattc
      181 cactgccctt tttatcatta actttgccac ttcacccatc catgcagaaa atacactcat
      241 tacattacac tataaaaaag aaatcatttc cttattcctg gaaaccacgc ctattaaagc
      301 taaccatttg cgaagcatac tggattatat tgaacaggaa cagttaactg ccgaaaaacg
      361 taaccattgc atgaaactgt ccaaaaaaat tcatagagaa aaaaccatac agccaacagt
      421 aaatctcaat gatagtgcat tttttcgcaa tctccttctg acactatttt ttgtcgccat
      481 ccgtcattac aatacgccct tgattcattg cgaaatggaa aaggcaaagt caacctgatt
      541 aaacattact cctctgttga atccatacag cagcatgttc ccttagtccg ggacgcggaa
      601 ttcagagcat tacttcgcca tcctcttgca aggagtcgcg ttatcgcgag tcaggatttt
      661 ggctttgctt tagatatttt cttctgtcga atgatggcaa acaatgtcag t
//
LOCUS       DAZFRG010000598          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFRG010000598 DAZFRG010000000
VERSION     DAZFRG010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.601"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..576
                     /gene="fimA"
                     /locus_tag="ACHKUC_005280"
     CDS             13..576
                     /gene="fimA"
                     /locus_tag="ACHKUC_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6621301.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          177 a          142 c          179 g          211 t
ORIGIN      
        1 ttttggagaa ttatgaaact caaacatgtc ggtattattg tcgtttctgt gttggcgatg
       61 tcgtctgctg cggtaagcgc agccgagggt gatgaatcag taacgaccac tgttaatggc
      121 ggtgttattc attttaaagg tgaagtggta aatgccgctt gtgcgattga ttcagaatca
      181 atgaaccaaa cggttgagct gggtcaggtc cgttcttctc gcctggctaa agcgggtgac
      241 ctcagctccg ccgttggctt caatatcaag ctgaatgatt gtgataccaa tgtttccagt
      301 aatgcagctg ttgcattcct gggtactact gtcaccagta atgacgatac attagcgctg
      361 cagagttcag cggcaggctc tgcccaaaat gtcggtatcc aaattttgga ccgtacaggg
      421 gaggtattag tacttgatgg tgctactttt agcgctaaaa ctgacttgat tgatggcacg
      481 aatatactgc cgttccaggc tcgttatatt gctctcgggc agtcagtagc tggtactgca
      541 aacgctgatg cgaccttcaa agttcaatat ctataattca ctcttaaggt gactatgcgt
      601 catctgaaat tgaaacaggg tgtgtgtggc atggctacgc acacccgaga aattcaccaa
      661 tgttcatctc attattaatg aaaaatggtt tgtattttgg agcggtatg
//
LOCUS       DAZFRG010000599          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFRG010000599 DAZFRG010000000
VERSION     DAZFRG010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.602"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..691
                     /gene="ompL"
                     /locus_tag="ACHKUC_005281"
     CDS             8..691
                     /gene="ompL"
                     /locus_tag="ACHKUC_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO6621302.1"
                     /translation="MNAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVGYNF
                     DMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGSGGA
                     VYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKFSYT
                     FEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHREQNQ
                     IRIGTKYFF"
BASE COUNT          220 a          144 c          142 g          202 t
ORIGIN      
        1 gaaaaatatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg tattcgctgg
       61 cgcatacgtc gaaaacaggg aagcatataa tctcgcctcc gatcagggtg aggttatgct
      121 acgtgtgggt tataacttcg atatgggcgc gggtattatg ctaaccaata cctacacctt
      181 tcagcgagaa gatgaactca aacatggcta taatgaaatt gaaggttggt atcctttatt
      241 taaacctacc gataaattaa ccatccagcc cggtggctta attaatgaca agagtatcgg
      301 ttcgggtggt gctgtttatc tggatgttaa ctacaaattt actccgtggt ttaacctgac
      361 ggtgcgtaat cgctataacc ataataatta cagctctaca gatttgaacg gcgaattgga
      421 taataacgac acctatgaag ttggcaccta ctggaacttt aaaataacag aaaaattttc
      481 ctatacattt gaaccacatt acttcatgcg agtgaatgac tttaatagta gcaacgggaa
      541 agatcatcat tgggaaatca ctaacacctt ccgttaccgt attaatgaac actggcttcc
      601 ttatttcgaa ttgcgctggt tagaccgcaa cgtcgagccg taccaccgcg agcaaaacca
      661 gattcgtatc ggaacgaagt atttcttctg atatgaaaat gtaaaaag
//
LOCUS       DAZFRG010000600          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFRG010000600 DAZFRG010000000
VERSION     DAZFRG010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.603"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..407)
                     /locus_tag="ACHKUC_005282"
     CDS             complement(<1..407)
                     /locus_tag="ACHKUC_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO6621303.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQAL"
     gene            complement(603..>706)
                     /locus_tag="ACHKUC_005283"
     CDS             complement(603..>706)
                     /locus_tag="ACHKUC_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6621304.1"
                     /translation="ERSALLYGLIGTCLLNDIDPEAYLRHILSVLPE"
BASE COUNT          213 a          141 c          151 g          201 t
ORIGIN      
        1 agcgcttggt tacagccttt tccccccggt gaaaatataa aattacttgc cagaagcgat
       61 tctccgatag tgggaagcct cggcaaataa ctgatgatat caacattaaa cgaaccaata
      121 acgcatactt gacttttcat agttgagtgg ccttttttta ttccctgggc tgtatcataa
      181 ttttcaagcg cataagcgat ttcatttttc gcaatagtat caagcgagtc taggacgata
      241 aaagcaccgc catgacatcg agtcagatag ccaagcctgg caagttcatt aagatctgat
      301 cgaatagttt ctttcgttac attgaggcta ttggacagat cggatacttt tacagaaccg
      361 aatttactga gtcgtttcaa tatttcatga tggcgttcaa atttcatgct tcacctcatt
      421 tcagttacgt taatatctat cgatgtatat tgatgtgata atcaccataa agtggcgggg
      481 aaaagcaaaa aaaagcaaaa atagcctgag aaaaggtaag cgttaatgga gaatcatatt
      541 gatgcttatt tattggtgag aactacgttc catggcagga gttcatcaat acggttggaa
      601 ggttattccg gcagtacgct caggatatgg cgcagatacg cttccggatc aatgtcgttc
      661 agaaggcaag tgccgataag cccatacaac agtgcactgc gctcgc
//
LOCUS       DAZFRG010000601          705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFRG010000601 DAZFRG010000000
VERSION     DAZFRG010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.604"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..308
                     /locus_tag="ACHKUC_005284"
     CDS             <1..308
                     /locus_tag="ACHKUC_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621305.1"
                     /translation="IQDELQALLDANNRYKWDSAHEMGHKVLDEYGEGSSPDYSWTHK
                     GTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYTRLVAADEDVQGLIWLSRIKFDD"
     gene            301..>705
                     /locus_tag="ACHKUC_005285"
     CDS             301..>705
                     /locus_tag="ACHKUC_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621306.1"
                     /translation="MTRVVIALIFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTANKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDS"
BASE COUNT          231 a           95 c          155 g          224 t
ORIGIN      
        1 atattcaaga tgaattacag gcgcttcttg acgctaataa cagatataaa tgggatagtg
       61 ctcatgaaat ggggcataaa gtactggatg aatatggaga gggttctagc cctgattatt
      121 cgtggactca taaaggaaca tctactctta tgcaaaaaac aataccaggg aatgttatgc
      181 ccgctcaggg agagattgat gtgatgaaat atggaaaata tcgtcctgac atgtatactc
      241 gattggttgc agcagatgaa gatgttcagg gattgatctg gttaagcagg ataaaatttg
      301 atgactagag tagttattgc tttaatattt cttgccggat gtacttcgca aatgaagtat
      361 tccaatgtcg tcaggattag gaatgtatgt tcctcagata ttaatgtgtt tattcctgag
      421 tatgtttttg gaggtaatgt tattaagcaa catcatattt acttacctgt taatgggatt
      481 gatgttttgt cttattttta tactgaaaac atcaatgatg gtttccaata tgaagaagca
      541 ttcaccgcaa ataaagatag ctcgggtaat tttgattatc cgtttgttat cttatcatca
      601 ggacgtgaaa aaaaatttgg gaaaaacgat ctaattgaat tatccaggga catttcaaca
      661 aaagacgata agaagcatgg ggttgtaaag tacatgatag atagt
//
LOCUS       DAZFRG010000602          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFRG010000602 DAZFRG010000000
VERSION     DAZFRG010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.605"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>704)
                     /locus_tag="ACHKUC_005286"
     CDS             complement(<1..>704)
                     /locus_tag="ACHKUC_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HFO6621307.1"
                     /translation="FHITSISLFLIMLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYS
                     TIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKA
                     IASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTPGFFLSTSIKDKGKI
                     VGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMRTLQPLPVQAKQKLQ
                     STRQYSLDNLLPADYY"
BASE COUNT          203 a          126 c          157 g          218 t
ORIGIN      
        1 ataataatcc gccggtaaaa gattgtcgag actatattgg cgggtagatt gtagtttttg
       61 ttttgcctga acaggtaacg gttgcagtgt tcgcattcgc catggcgatt ttgaacttaa
      121 aaatataatc ccatgttcat cattcacgat aatattttca ggaccttcgg cccatgcttt
      181 ttcaatttca ttaagactta tttttactac tacaacaccg acaatttttc ctttatcttt
      241 tatacttgta gagaggaaga atcccggtgt attcgtagtg ctaccaatac cgtaaaagcg
      301 tccatttaag ccagacatgg cgtgtttata ataggggcga tagctataat tttgccctac
      361 atagctgccg gggtcctgcc agttgctaga agcgatggcc ttaccttggg tatccagaat
      421 aaatattgct gcggttttga gttgagtatt gaaatgtgca attcgttgat tgagttcgct
      481 ggacgtcata tcgtcaggcg taattaccgc ctgacggatg atatggtctg ttgagagcat
      541 atagggtaaa acataaaatt gctcgatggt ggaatataat gtggacttat acatttcaag
      601 ccgcatttta tcggtaccgg acaaggtatt aaaacgcaag tgcaaaaaag cgattgaaac
      661 cgcgagcatg ataagaaaaa gtgaaatgct tgtaatgtga aatc
//
LOCUS       DAZFRG010000603          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFRG010000603 DAZFRG010000000
VERSION     DAZFRG010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.606"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..443)
                     /locus_tag="ACHKUC_005287"
     CDS             complement(<1..443)
                     /locus_tag="ACHKUC_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFO6621308.1"
                     /translation="MADSTRNRSAERLIDILVELQNYGVVSRHNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARTDNQNKESLHKSLARLLDTDAIFPERDENFWQKLE
                     NRATEEHIRVQFHNPEHTIRDDLRKYLGVLEKAIRNSNVCQITYAG"
BASE COUNT          195 a          162 c          126 g          221 t
ORIGIN      
        1 ccggcatatg taatctgaca aacattactg ttgcggatcg ctttttcaag aacaccaaga
       61 tatttcctta aatcatccct gatggtgtgc tcagggttat gaaactgaac acgaatatgc
      121 tcttcggttg ccctgttttc taatttctgc cagaaatttt catccctttc aggaaaaatc
      181 gcatcagtat ccagtaacct ggccagagat ttgtgcagtg attctttgtt ctggttatct
      241 gttcttgctg aaataagccg gtattttcca tcaccacagg cttcaataaa gggagaaagc
      301 atgttcagat cacgatatgc tgttcgctca gtgatgttgt attttttcat caaattatgg
      361 cgactgacca cgccataatt ctgtaattca acaaggatat caatcaaacg ctccgctgag
      421 cgatttctcg ttgagtcagc cataaatcac tgcacctgta gtattactct tgtgtaagtg
      481 aattatataa atacccctga catcatttgt cagttccaca aaactacgcc ttccggcaac
      541 agatttttat ttgattatgt tcaaacttta aaccagatgt ccggggcctc cttcgtaata
      601 cggtttatta tttccggaag ggctgatatg gcaaaaaaca acgtatccca acaacagtcc
      661 tttttcattt aacagactga caaccatact gcgcgctacc ctgc
//
LOCUS       DAZFRG010000604          700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFRG010000604 DAZFRG010000000
VERSION     DAZFRG010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.607"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            225..>700
                     /locus_tag="ACHKUC_005288"
     CDS             225..>700
                     /locus_tag="ACHKUC_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6621309.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGS"
BASE COUNT          209 a          147 c          157 g          187 t
ORIGIN      
        1 cctttagcaa aggaaaagtt gattcagata taatttagca tttcaatcat ctgacatcat
       61 tataaaaaat gagattcaca taaagataaa attattgttc tcaaggaaag catgaagggg
      121 cttcgtaagc aatattcatt cagtgccaag acaggacttg atacacagaa tctcaacttt
      181 tatgctctca gactatatca ccacatgttt tacaggctca gaaaatgaac gatagattat
      241 gctttgaagt gcacgataac aagggttatt tcgtatttcc ggatacctgg tttggcccat
      301 tgctggggga gtttgaagaa gtgcttgatg catacgacgc tgatgaaatc agcgagacga
      361 gttacatcaa caaactccga cgccttgctc aacgggagcc ggactttatt gatatacacg
      421 ctcatctggc atatgcattt ctggaacaga atgccccgcg taaggcactg aacgccgctc
      481 ttaaaggact ggcggcaggt aatcgtatca tcccggaaag tttctgtggt gaaataatct
      541 ggatgcatcc cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg
      601 tgcacttaca gcgccatcag gatgcggtta tgctcactga taaaatactg gcatacaatc
      661 ctgaagataa tcagggggct cgctggttgt tgggttcaga
//
LOCUS       DAZFRG010000605          694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFRG010000605 DAZFRG010000000
VERSION     DAZFRG010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.608"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(389..>694)
                     /locus_tag="ACHKUC_005289"
     CDS             complement(389..>694)
                     /locus_tag="ACHKUC_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621310.1"
                     /translation="DLRKAIYSDRMLSRLADSGNIVIHSSLGYPFAKYKNTGISIGIE
                     PLNPMIRQDLTLGYIVVVRNRKASQEVNGLLNRSLPKAISTFNDHINEYESVKIKMR"
BASE COUNT          209 a          122 c          132 g          231 t
ORIGIN      
        1 aaaaccaatg gaaacgactt ctatcgttga tttttcagag caagtcaaag ccattgtaga
       61 agattttcaa actggcagaa aaaaatgggt tttcaatgat ggaaatgtaa gataaaaatt
      121 agtctggcga ttagtaaaat atagccagaa agatacttgc cagcgttgtt ccttcttcaa
      181 tcagaacggt atggtggaag aaacggagaa gaaagaacgg atcggtatcg gctatgacgt
      241 tctggatgga tagcaatgga acggcagcgg tttcatcgta attggattga aaccgtagta
      301 ttgcatcgga acaaaaatta acttaccttc catcgctcat cctcctcctg tagttatcag
      361 actaaatatt ttaccagatc ataggttgtt atcgcatttt gatttttact gattcatatt
      421 cgttgatgtg atcattaaaa gtgcttattg ctttgggcaa tgacctgtta agtaatccat
      481 taacttcttg tgatgctttt ctgtttctga ctaccacgat atatccaaga gtcagatctt
      541 gtcttatcat tggatttaat ggttctattc ctatgctaat ccccgtattt ttatatttag
      601 caaacggata tccaagtgaa ctatggatta caatattacc gctatctgca agccttgata
      661 acattctgtc gctgtatatt gcttttctta aatc
//
LOCUS       DAZFRG010000606          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFRG010000606 DAZFRG010000000
VERSION     DAZFRG010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.609"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..619)
                     /gene="ipbA"
                     /locus_tag="ACHKUC_005290"
                     /gene_synonym="fimX"
     CDS             complement(56..619)
                     /gene="ipbA"
                     /locus_tag="ACHKUC_005290"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO6621311.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          214 a          145 c          143 g          187 t
ORIGIN      
        1 tggaagaatt gaggaaaacg taggaatctg acattttgaa tcagaaggta ctgttttaac
       61 gaggcttttt tttccacacc cctttaaaac gagcagcatt actggctgtg tatctgacag
      121 tatgctgaat atttcgatga cccagataat cctgaagtag tcgggtatcg acaccattgt
      181 cagccagagc aaaaccacag gcatgacgta acatatgagg atgtgctttt acagctaatc
      241 ctgcacgttt tccagcctca cgaatgatag aaaaaaaatg ttgtctgctg atagggcggc
      301 gtttccggga gagaaaaagc cagtctcctt caactccgtt ttcaattaac ttcctctgct
      361 taagccacaa cttgatcaaa ttatattcat caggaagaag aggatgtgtc gtcgagaaac
      421 catttttaat acgccgaata ttaagttgtt ttcctgatgc atcaatatcg gataaacgta
      481 aatcaagaag ttcactggcc ctaaaaccgt gaatgaaagc catcataatt agacagcgat
      541 ttctttcagg aaaggacatg ctctgggctg catccatcag cctgtagact tcatcctgtg
      601 tgagatattt tctcgtcata cgccaccgtg tacagttgta aaaaaaagag aggcacaccc
      661 agataagtat gcctgcaatc agaacgtta
//
LOCUS       DAZFRG010000607          687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFRG010000607 DAZFRG010000000
VERSION     DAZFRG010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.610"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..284
                     /locus_tag="ACHKUC_005291"
     CDS             3..284
                     /locus_tag="ACHKUC_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621312.1"
                     /translation="MLHAICAVAVQGLVGCITGDWAYGAAIACTFFVAREYTQAEYRW
                     IKRFGDGHRHKMPWWGGFDPHAWDVASLMDFVVPVVACAGLYGCMLIFS"
     gene            complement(402..>687)
                     /locus_tag="ACHKUC_005292"
     CDS             complement(402..>687)
                     /locus_tag="ACHKUC_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6621313.1"
                     /translation="FRVSFYWVETDDAGGTIPVTMTEQISPYHQCMQGTNQKNPRCVA
                     LIGQPGVNAYCSIYEHRSSTCREFPMSGEHGIVNEACNRARAKYGLPPLY"
BASE COUNT          149 a          141 c          207 g          190 t
ORIGIN      
        1 cgatacttca tgcaatttgt gctgtggcgg tacaggggct ggttgggtgc attaccggtg
       61 actgggctta tggtgcagcc attgcctgta cgttttttgt tgcccgcgaa tatacccagg
      121 cagaatatcg ctggattaag cgttttggtg atggtcaccg acataagatg ccgtggtggg
      181 gcggttttga tccgcacgcg tgggatgtgg caagcctgat ggattttgtg gtgccggtgg
      241 tggcctgtgc gggattgtat ggctgtatgc tgatttttag ctgaaaaaaa gggagcgctc
      301 aggctcccca atcggaagaa atcgcttgaa acgacaagct tgttgttacg ccctctaaga
      361 atgttatata aaacatgcca ttgccgtgat tatgatcgcg tttaataaag agggggtagc
      421 ccatattttg cacgagcgcg gttgcatgct tcattcacga tgccgtgttc tccggacatc
      481 ggaaattctc tgcaggtgga tgagcgatgt tcataaatgc tgcagtaggc attgactccc
      541 ggttggccga tcagggccac acagcgagga tttttctggt tagtgccctg catgcattga
      601 tggtagggag agatttgttc tgtcatcgtt acggggatgg tgccgccagc atcatctgtt
      661 tcaacccagt aaaaagagac gcggaaa
//
LOCUS       DAZFRG010000608          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFRG010000608 DAZFRG010000000
VERSION     DAZFRG010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.611"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..678
                     /locus_tag="ACHKUC_005293"
     CDS             <1..678
                     /locus_tag="ACHKUC_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO6621314.1"
                     /translation="YSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACW
                     AHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKS
                     LESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLG
                     RKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPW
                     RVALPTE"
BASE COUNT          167 a          190 c          212 g          117 t
ORIGIN      
        1 tacagcccgg acagaaaagg catccatccg cagacccatc ttgcggggtt cagtggtgta
       61 ctgcaggcgg atgcatacgc cgggttcaac gagctgtacc gggatggccg gataacggaa
      121 gccgcctgtt gggctcacgc ccgccgtaaa atccacgatg tgcacgttcg caccccgtca
      181 gccctgacgg aggaagcgct gaaacggatc ggcgaactgt acgccatcga ggcagagata
      241 aggggaatga cggcggagca gcgccttgcc gaacgtcagt tgaaaacgaa accgctgctg
      301 aaatccctgg aaagctggct gcgtgaaaag atgaaaaccc tgtcgcgaca ctcagaactg
      361 gcgaaagcgt tcgcatacgc cctgaaccag tggccggcgc tgacgtacta tgcagatgat
      421 ggctgggctg aggcggacaa taacatcgct gaaaatgcgt tgcggatggt cagtctgggc
      481 cgcaaaaact acctgttctt cggttcggat catggaggag agcggggagc gctgctgtac
      541 agcctgatcg ggacgtgcaa actgaacgga gtggagccag aaagctacct ccgctatgtc
      601 cttgacgtca tagccgactg gccgataaac cgggtcggcg aactgctccc ctggcgcgta
      661 gcactgccga ctgaataaca catccc
//
LOCUS       DAZFRG010000609          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFRG010000609 DAZFRG010000000
VERSION     DAZFRG010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.612"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(36..686)
                     /locus_tag="ACHKUC_005294"
                     /pseudo
     CDS             complement(36..686)
                     /locus_tag="ACHKUC_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          159 a          180 c          159 g          188 t
ORIGIN      
        1 tgaattcgac gcggtttttt agctaacggg cgggatcaga acttatattt caggcccacc
       61 atcattgcgg tgtcattgta gccattatca cccagctgca cgcccacatt accccagacg
      121 ctggccgccg gattcagatt gccatttacg ccatgtttca tttcccccag attacgcgcc
      181 ccgtcttgtt caactttcac accgtttatt gagacagcaa aatctttact gttatggatc
      241 ccgttcattt caacaaaagg ttcaaagccg tcaccgtgcg gaccgctgtt tttctcctga
      301 gtacgaataa aggtgcggaa aaccaagtcg gatctgaatg ttgttatttg cgtcattctc
      361 aatacgtgag ccattggctt ccgtgtgcgc attatgatcg acgcccatcc agatggcctg
      421 tgccgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg
      481 acgtaaccaa agttggcttg aacgctcctg ttcagttaca ggatcgatat agcgcgtttc
      541 acctgcacaa tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac
      601 gtaactgcca gcttcgggtc gcaggacggg ggtaggcgtt tctgttgatt ctgaagtgac
      661 attggaaaaa aataactctc taccat
//
LOCUS       DAZFRG010000610          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFRG010000610 DAZFRG010000000
VERSION     DAZFRG010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.613"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            141..>682
                     /gene="papA"
                     /locus_tag="ACHKUC_005295"
     CDS             141..>682
                     /gene="papA"
                     /locus_tag="ACHKUC_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
                     /protein_id="HFO6621315.1"
                     /translation="MIKSVIAGAVAMAVVSFGVNAALTTPQGQGRVTFNGTVVDAPCS
                     ISQKSADQSIDFGQLSKSFLQAGGVSKPMNLDIELVNCDITSFKGGGGVSAAKKGTVK
                     LAFSGPRVSGHNEELDTSGGTGTAIVVQAAGKNVSFDGTEGDANTLKDGDNVLHYTAI
                     VKKSSANNAQVTEGAFSAVAT"
BASE COUNT          186 a          112 c          177 g          207 t
ORIGIN      
        1 tcagtattct gtcgataaat aacctgccct gaaaatacga gaatattatt tgtattgatc
       61 tggttattaa aggtaatcgg gtcattttaa attgccagat atctctggtg tgttcagtaa
      121 tgaaaaagag gttgttattt atgattaagt cggttattgc cggtgcggta gctatggcag
      181 tggtgtcttt tggtgtaaat gctgctctaa ctactccaca ggggcagggt agagtaacct
      241 ttaacggaac tgttgttgat gctccatgca gcatttctca gaaatcagct gatcagtcta
      301 ttgattttgg acagctttca aaaagcttcc ttcaagcagg aggcgtatcc aaaccaatga
      361 atttagatat tgaattggtt aattgtgata tcacttcatt taaggggggg ggaggagtct
      421 cggcagcaaa aaaagggact gtgaagctgg cttttagtgg tccaagggtt tctggtcata
      481 atgaggagtt agataccagc ggggggacag gtactgcgat tgtagttcag gctgcaggta
      541 aaaacgtttc tttcgatggc acagaaggtg atgctaatac cctgaaagat ggtgataatg
      601 tgctgcatta tactgcgatt gttaagaagt cgtcagccaa taatgcccaa gtcactgagg
      661 gtgctttttc tgcagtcgca ac
//
LOCUS       DAZFRG010000611          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFRG010000611 DAZFRG010000000
VERSION     DAZFRG010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.614"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..299)
                     /locus_tag="ACHKUC_005296"
     CDS             complement(<1..299)
                     /locus_tag="ACHKUC_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6621316.1"
                     /translation="MAAVKISGVLKDGTGKPVQNCTIALKARRTSSTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVR"
     gene            complement(364..>680)
                     /locus_tag="ACHKUC_005297"
     CDS             complement(364..>680)
                     /locus_tag="ACHKUC_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6621317.1"
                     /translation="WFSVMAGPSVRVNEWFSAYAMAGVAYNRVSTFFGDYLRVTDNKG
                     KTHDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGY
                     KF"
BASE COUNT          157 a          219 c          157 g          147 t
ORIGIN      
        1 cgaacatcat cctccgtcat ggcgccgaga aaatcattca gcgtacctgg tctggaacct
       61 tcatagacgg taatggtccc ggcatgtgaa ggcggaaaac cttcaaccag cagggtgacg
      121 ctgtactggc catactcaac atccatgctg taacgcccgg cttcatccgg attttcagag
      181 gccaccgtgt tcaccaccac cgtgctgctg gtccgtctgg ccttcagcgc aatggtgcag
      241 ttctgtactg gttttcctgt gccatctttc agcacacctg agatttttac tgctgccata
      301 tccactccac aaaaaagccc gcctgaaccg gcgggctgtc ataacactgt gttacctggc
      361 taatcagaat ttataaccga cacccacgat gaaaccgtca gtgcgccagt caccactgcc
      421 ggaaccttca taagcaaggt caatggccac ggattcggtc gggttaaact gcacgccagc
      481 cccccacgcc agagacgtgt tgctgtggcg accgtcatca cttccggtca gcacatcgtg
      541 cgttttcccc ttgttgtcag ttacgcggag ataatccccg aagaaagtcg acacacggtt
      601 gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgca cagacggccc
      661 cgccatcacg ctgaaccagc
//
LOCUS       DAZFRG010000612          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFRG010000612 DAZFRG010000000
VERSION     DAZFRG010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.615"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..668
                     /locus_tag="ACHKUC_005298"
     rRNA            <1..668
                     /locus_tag="ACHKUC_005298"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          152 c          223 g          143 t
ORIGIN      
        1 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
       61 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      121 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      181 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      241 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      301 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      361 gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt
      421 acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc
      481 cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga
      541 gatgagttct ccctgagact tagagtctcc tgaaggaacg ttgaagacga cgacgttgat
      601 aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgaggc
      661 ttaaccttac aacgccgaag
//
LOCUS       DAZFRG010000613          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFRG010000613 DAZFRG010000000
VERSION     DAZFRG010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.616"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          199 a          178 c          127 g          172 t
ORIGIN      
        1 cacttaagta aaatacgtgt atagtaccat tcccgacatt ctcaacatct tcagacatcc
       61 atgcagactg agtgttgatt tcagtttttc acggtcagca tggttctttg ctgtttaaaa
      121 aacatacaga tttactctga aattatttac ccaggcctcc ctgaaaagac catccggagc
      181 atcccgttcg tcagcttcca tgttgtcttt taccccggaa agtgatcgca ttttccagcc
      241 agaatgacag ccatactgat tatgacgtct ctccggcccc ttcacatata acattcatgt
      301 cagtcatttc ttactgcctc actcaccgga attctccatg ccggatgaag caattgttct
      361 gaactgagca gaagcataac agcacacaag gaaacgactg tgctatagat cctggaatca
      421 ggatcttaat gtcaggaaaa caatgacgac cggggataac caacaacaac ctgagtacag
      481 aagaaagctt tcccgcgcac gccatcatct tatgtaagag gatatgatgc cacccccacc
      541 tcagaagaac cgtctccggg agaacttcac tgcatccaaa atccttttgc tgagttctca
      601 ttccccatca tggcagctac agacatgccc tgacagggcg tatgcgcatt gagagaaata
      661 accataaatg atgaga
//
LOCUS       DAZFRG010000614          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFRG010000614 DAZFRG010000000
VERSION     DAZFRG010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.617"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..606)
                     /locus_tag="ACHKUC_005299"
     CDS             complement(<1..606)
                     /locus_tag="ACHKUC_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6621318.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAEN"
BASE COUNT          211 a          126 c          158 g          180 t
ORIGIN      
        1 attttccgcc aaatatcgcg ttgcagaaag tcggcaaata attaacactt tgtctccttt
       61 agtgaaagtt tttccttctt caggctccag tagcaaataa tgtatttggc caaattgatc
      121 ggtgagtttt ccttcgcaag ggttacctga agtggcttga tggccggtaa ttaatgccat
      181 gctaccaaca tattcttctt ctgtaatagc tgaactatgg tcgcgtggaa tccagggtgc
      241 aacgacttta ccggtataat gcactgcaat aattgtaaac agtaaactga caggaaccac
      301 gaacaggttg gggagtggtg attgccagag cgtaacgcag gcatgttgca acaggatgcc
      361 aataagacca aaaaaaccag cgagtaagca gaggacgacg agagctggta atcttccaat
      421 attgagataa tgaagtgcct ggctaatatg acctgtggtt atggaatcat aatggtcaag
      481 atgtgcatca agagcaccgg agagcatgtg accgcagata agtgcaaaaa tttctaaaag
      541 gccgatcaag agtacaaagg aaatagcgaa aagataaggg gtgttatagt cggcgaataa
      601 aatcatatgt cactccaatg tccatttagt gacgcaatat ttactttgga atagtttcct
      661 tgcttacatt tgtct
//
LOCUS       DAZFRG010000615          672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFRG010000615 DAZFRG010000000
VERSION     DAZFRG010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.618"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..561
                     /locus_tag="ACHKUC_005300"
     CDS             <1..561
                     /locus_tag="ACHKUC_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6621319.1"
                     /translation="SSDNNDFRARGRGWQGSLETGLPFSITDNLMLEPQLQYTWQGLS
                     LDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPV
                     NWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGV
                     QAGYAHSVSGNSAEGYNGQATLNVTF"
BASE COUNT          149 a          198 c          194 g          131 t
ORIGIN      
        1 tcatcggaca ataacgactt ccgcgcccgg ggccggggct ggcagggctc actggaaacc
       61 ggtctgccat tcagtatcac tgacaacctg atgctggagc cacaactgca gtatacctgg
      121 cagggacttt ccctggatga cggccaggat aacgccggtt atgtgaagtt cgggcatggc
      181 agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat gacctttggt
      241 gaaggcacct catcccgtga caccctgcgc gacagtacaa aacacggtgt gagtgaactg
      301 cctgtgaact ggtgggtaca gccttctgtt atccgcacct tcagctcccg gggagacatg
      361 agcatgggta cagccgcagc cggcagtaac atgacgttct caccgtcccg gaatggcacg
      421 tcactggacc tgcaggccgg actggaagcc cgtgtccggg aaaatatcac cctgggcgtt
      481 caggccggtt atgcccacag tgtcagcggc aacagcgctg aaggctataa cggtcaggcc
      541 acgctgaatg tgaccttctg acagaaccat cgcctctttg tggtcccgat catcatgatc
      601 gggacccgga ccggcgcaac ggatattcaa cgccacattc gctggcatta acagtgcggc
      661 attcgacgga ca
//
LOCUS       DAZFRG010000616          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFRG010000616 DAZFRG010000000
VERSION     DAZFRG010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.619"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..662)
                     /locus_tag="ACHKUC_005301"
     CDS             complement(<1..662)
                     /locus_tag="ACHKUC_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6621320.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AI"
BASE COUNT          213 a          160 c          149 g          146 t
ORIGIN      
        1 aatatcgcga agtagagata ctctttggca atatgaatgt tgagactatc ggcaaccagc
       61 aatacaccga caaacaataa aaaggttaac gccagtattt ttattgacgg cgtggcgtta
      121 acgaaatcac caattgtttt cgccgcaaac atcatgacac cgacggcaat cattacggcg
      181 gcaatcatga taaatatatg ctgagaaagg ccgacggcgg tgatcaccga atccaggcta
      241 aacacgatat cgagcagcat tatctgtaca atggcaccgc acaacgacag ttgccgcgat
      301 ccgctgccat gttcgtcttc ctgcgcgggc gacagctctt cgcgtatctc ctgtatcgct
      361 ttataaatca ggaacagccc gcccccgagc agaatcaagg tgcggaacga aatctccagc
      421 gtcagcacgg taaaaagcgg ctgggtaata tgtgaaagcc acgcaatgga tatcagcaat
      481 aatatccgca taatcatcgc gcagcttaaa cccagtttac gcgccatatt ttgttgtgat
      541 aacggaagtt ttgccaccac cagtgaaaga aaaataatat tatccacccc aagaataatt
      601 tccagaaagg tgagggtaat taaagtgagc cagatatctg gctgcataaa ccacaaaaac
      661 atggggtg
//
LOCUS       DAZFRG010000617          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFRG010000617 DAZFRG010000000
VERSION     DAZFRG010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.620"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>664
                     /locus_tag="ACHKUC_005302"
     rRNA            <1..>664
                     /locus_tag="ACHKUC_005302"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          161 a          151 c          221 g          131 t
ORIGIN      
        1 gggcctcttg ccatcggatg tgcccagatg ggattagcta gtaggtgggg taacggctca
       61 cctaggcgac gatccctagc tggtctgaga ggatgaccag ccacactgga actgagacac
      121 ggtccagact cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat
      181 gcagccatgc cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg
      241 aagggagtaa agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa
      301 ctccgtgcca gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactgggcg
      361 taaagcgcac gcaggcggtt tgttaagtca gatgtgaaat ccccgggctc aacctgggaa
      421 ctgcatctga tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg
      481 gtgaaatgcg tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgaagac
      541 tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc
      601 cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga gctaacgcgt
      661 taag
//
LOCUS       DAZFRG010000618          654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFRG010000618 DAZFRG010000000
VERSION     DAZFRG010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.621"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..654
                     /locus_tag="ACHKUC_005303"
                     /pseudo
     CDS             1..654
                     /locus_tag="ACHKUC_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          219 a           90 c          159 g          186 t
ORIGIN      
        1 aaatcaaaaa taatacttct acaagtaatg gggagtatct aatcacttaa gacgaaagcc
       61 atgccgtgaa agtggatgac ggtgtaactg gatatataca taatgccagt gtgatgacta
      121 gtggtgatgg atcttatggt atttctgttg atagccaaaa caaagtatta tatataagcg
      181 atagcaatat taagacgctc tgaaagcgta tctgacaaag aaaatggagg gataacagcc
      241 agcgcagtag tcagtgaatt tggtggcacc atcgttatga atggtgataa ttcagtcgag
      301 acgagggggc atattcagcg ggacttttaa gtcaggttaa tgattctgag actgtggaaa
      361 ataacaccag tcttgaaacc acagataaaa cgaacattgt tacctatggg aaaaatgcag
      421 taggagttct tgcatgttca agtcctggag agtctcgaac atgtgtcgat gctgtagatg
      481 atgaagtttg tgattctaac agttacgaag ttattagccg tgctgattta aaaatgaatg
      541 gtggttccat aacaactaat ggtattaata gctatggtgc ttatgctaat gggaaaaaag
      601 catatattaa tttagattat gtggtacttg aaactgtggc tgatggaagt tatg
//
LOCUS       DAZFRG010000619          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFRG010000619 DAZFRG010000000
VERSION     DAZFRG010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.622"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..630
                     /gene="iha"
                     /locus_tag="ACHKUC_005304"
     CDS             <1..630
                     /gene="iha"
                     /locus_tag="ACHKUC_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO6621321.1"
                     /translation="AGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHGVEFA
                     GTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLG
                     ARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNAAVNN
                     LLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
BASE COUNT          187 a          142 c          174 g          150 t
ORIGIN      
        1 gccggtctga atgccaatgt cacaggtttt atgactgact tctccaacaa gattgtctct
       61 tattccataa atgataacac caatagctat gtaaacagcg gaaaggcccg gttgcatggt
      121 gtggaatttg ccggcacatt gccgctgtgg tcagaggatg tcacgctgtc actgaattac
      181 acctggaccc gaagtgaaca acgtgatggt gataacaaag gtgcgccgct gagttatacc
      241 cctgaacaca tggtgaatgc gaaactgaac tggcagatca ccgaagaggt ggcatcatgg
      301 ctgggtgccc gttatcgcgg gaaaacacca cgtttcaccc agaattattc gtcactgagc
      361 gctgtacaga agaaagtata tgatgagaaa ggagaatacc tgaaagcctg gacggtggtg
      421 gatgcaggtc tgtcgtggaa gatgacggat gccctgacgc tgaatgctgc ggtgaataac
      481 ctgctcaaca aggattacag tgacgtgagc ctgtacagtg ccggtaagag tacgctgtat
      541 gccggtgatt acttccagac gggatcatca acaacaggat atgtgatacc tgagcgaaat
      601 tactggatgt cgctgaacta tcagttctga taataacaaa acgctatcac tga
//
LOCUS       DAZFRG010000620          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFRG010000620 DAZFRG010000000
VERSION     DAZFRG010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.623"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..218)
                     /locus_tag="ACHKUC_005305"
     CDS             complement(<1..218)
                     /locus_tag="ACHKUC_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6621322.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVR"
     gene            complement(397..528)
                     /locus_tag="ACHKUC_005306"
     CDS             complement(397..528)
                     /locus_tag="ACHKUC_005306"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6621323.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGTHFRLVIRNAEGQRR"
BASE COUNT          150 a          157 c          172 g          158 t
ORIGIN      
        1 cgtacgcggg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag gctttccagt
       61 acggtgatgg tggggatgta cgtatagttc tggcttcggg acgtgtgttt atcttccccg
      121 aaaatccccg gcacatggtg catcagctct tcatgtgtca gtggacggtc acgacgtatc
      181 tggtttgcag cataaccaaa acgactggct agtcgcataa tttgctcctt atcggtggtt
      241 aagatttact ggtgtaataa atgaaaaaag ccgcgtctcc cggagaagac gcggcctgat
      301 ggatgaaatc aatgaggttt attgtctgag aagccctcca ctggcgagcc acgcattaag
      361 ctgttttccg gcatcagctt cagagttccg gctgcgtcag cgtcgctgac cttccgcgtt
      421 gcggatcacc agacgaaaat gcgtacccgg gtcatcctgg ataaacacat tgcggtaagc
      481 ggttatgaca gcaaccgcct gaccgtatga gaaagatcct tcagccagga catactctgt
      541 gtgtaacccg gcatatctgg tttctcctga taaatagcct ctgccatacg ttgtggcaga
      601 ggctgaagca tgaaactgac ttcagggatc agttaac
//
LOCUS       DAZFRG010000621          630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFRG010000621 DAZFRG010000000
VERSION     DAZFRG010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.624"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(4..558)
                     /locus_tag="ACHKUC_005307"
     CDS             complement(4..558)
                     /locus_tag="ACHKUC_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6621324.1"
                     /translation="MKKIAMIVAMLTVSASGFAASGDVIGTGNQNVTGSLNSSTCAVS
                     FPSDVTLPNITTADYASASQYGLIGNASVSAGNISFNGCTGQNVKVVINSPNNKSSNG
                     IMAFPTLNGSNQSVVGIALKEGISNKYVRVDGQESSNLMFTVDQDSFAIPVQVEALRA
                     SGSSMNSATSGTLSAQFTYTATYQ"
BASE COUNT          211 a          134 c          104 g          181 t
ORIGIN      
        1 attttattga tatgtcgcag tgtatgtgaa ctgagccgaa agtgtacctg atgtagcaga
       61 attcatggaa ctaccactgg cacgtaatgc ttctacctgt acaggaatag caaaactatc
      121 ttgatcgacc gtgaacatta agttcgaact ttcctgtcca tcaacacgaa catatttatt
      181 actaatacct tcttttaaag caataccaac tacagattgg ttcgaaccat tcagagttgg
      241 aaaagccatt ataccgttag aagatttatt gtttgggcta ttaataacaa cctttacatt
      301 ttgtccggtg caaccattaa aagaaatatt acccgctgaa acagaagcat ttccaattaa
      361 accatactga cttgctgaag cataatccgc tgtagtaatg tttggcagcg ttacatcact
      421 tggaaaagaa acagcacagg tagagctatt aagagagcca gtgacattct gattcccagt
      481 accaataaca tctcctgatg ctgcaaaacc tgatgcagag accgtcagca ttgcaacaat
      541 cattgcgatc ttcttcatat gatataatcc ttattaacaa aaacaaaaaa caaactttct
      601 ttccaaaggc atttcattgc cttgccaaat
//
LOCUS       DAZFRG010000622          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFRG010000622 DAZFRG010000000
VERSION     DAZFRG010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.625"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>628)
                     /locus_tag="ACHKUC_005308"
     CDS             complement(<1..>628)
                     /locus_tag="ACHKUC_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6621325.1"
                     /translation="GNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTT
                     KEAVLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNAS
                     LTMQGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDHKSNNQQTSF
                     DQPDWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAY"
BASE COUNT          158 a          116 c          119 g          235 t
ORIGIN      
        1 gtaagcattt ttatcgccga ttgtgatagc tgatttattt gctgatatat ttccttcaac
       61 attggcattg cgtgatattg aaaagtcagc attgttcagg tgtaatgtat caaatttaaa
      121 caccccggtt ttccagtctg gctgatcaaa tgaggtctgc tgattattac ttttgtgatc
      181 agaattgaac tttttattat atgaactctc tgtttctttc aaaacggtga cccagtccgt
      241 tccacaaaga aataccaggg agcaatgatt cgctgtgctt ctgaatattg catgctctgt
      301 tgcatggcct tgcatggtaa gactggcatt actgacttca acatcatttt ttgtgttgac
      361 actaccatcc agaattaatt ttgcattagt tttcttatcc tgagaattaa tattgtgcgt
      421 tagttttata ttgccattta tgttcccatg atatatgtag gagtcttcgt tattgattgc
      481 cagaacggct tctttcgttg tatctgagtt agttattgtt gttccatcgt cagtggcggc
      541 aattctagta aaagtctgat tgtgtccatt taaatctagc gtgccaccac gtttagtaaa
      601 aaatatcccc gcatattgat cattgcca
//
LOCUS       DAZFRG010000623          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFRG010000623 DAZFRG010000000
VERSION     DAZFRG010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.626"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(193..354)
                     /locus_tag="ACHKUC_005309"
     CDS             complement(193..354)
                     /locus_tag="ACHKUC_005309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621326.1"
                     /translation="MILRHRTGPAFQAIKDGLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(354..539)
                     /locus_tag="ACHKUC_005310"
                     /pseudo
     CDS             complement(354..539)
                     /locus_tag="ACHKUC_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          161 a          172 c          118 g          170 t
ORIGIN      
        1 tggtgtatga cacacctgtt gtgatttttt ccatctggta cataccccgt ctcccgttat
       61 ccggaagctg acaacaataa aaaagccacc agttaagtac tgatggctct gataactcat
      121 gcaggcgtct cagacgaccc actgacacta ccggtgagtt taacgatacc ttccatttga
      181 ctggctcact ttttatgatg atgccggtgc atttatctcc agcaccagac tttctatctc
      241 aacgccatac gttgcatttt tggtaatatc cgtcagcgtc agtgcattta gtcccactgc
      301 cagaccgtct tttatggcct ggaatgccgg gccagtacga tgacgtagta tcactccggc
      361 tcagttgcac cactgaccac cacatcacct tctgctgcaa tcgcctgcat cagggtataa
      421 ggggttatgg ccaccggact accaaacggc tgccagccct ctttcagttt atgtgtcagc
      481 ttttccgcaa gatctgacgg cgccgccgcc ctgacaacat catagtgttt aaatgccatg
      541 gttctttcca ccatctgaaa aataattctt taaaatacct gacatgtaat acagaaaaaa
      601 cacaaaacca taccttaaat a
//
LOCUS       DAZFRG010000624          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFRG010000624 DAZFRG010000000
VERSION     DAZFRG010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.627"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..539
                     /locus_tag="ACHKUC_005311"
     CDS             <1..539
                     /locus_tag="ACHKUC_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621327.1"
                     /translation="LRFEDATGGEQVYIHAQKNMDTEVLNNRTTDVKADHTETIGNDQ
                     KITVGLGQTVNVGSKKEGGHDQKVTVANDQHLTIKNDRHKVVNNNQTSKVTGTDTEEV
                     VKKQSIKIGDNYELKVEHGTNIISGDSIELICGQGESGTCSIKLEKTGKIIIRGTEFL
                     FEATGPVDIKGKDIHLNG"
BASE COUNT          211 a           97 c          174 g          132 t
ORIGIN      
        1 aactgcggtt tgaagacgcc acgggcggtg aacaggtcta tatccacgcg cagaagaaca
       61 tggatacgga ggtgctgaat aatcggacga cggatgtgaa ggctgaccac acggagacca
      121 ttggtaacga ccagaagata acggtggggt tgggccagac ggtgaatgtg gggagcaaaa
      181 aggaaggtgg tcatgaccag aaagtgacgg tagcgaacga tcagcatctc acgataaaaa
      241 atgaccggca caaggttgta aataacaacc agacaagcaa ggtgacggga actgataccg
      301 aagaagtcgt caaaaaacag agtattaaaa taggtgataa ttacgaactg aaagttgagc
      361 atggtacaaa tatcatatct ggggacagta ttgagctaat ttgtgggcag ggagagagcg
      421 ggacttgttc cataaaactg gagaaaacag gaaagataat aatacggggg acggagtttc
      481 tatttgaggc taccggtcct gtagatatta aaggaaaaga tattcatctg aacggatagt
      541 gcaggatata aaacatggtg attgtatttg ctttatacgc ctgtgaatca ccatttgtga
      601 ttgtttaaat taat
//
LOCUS       DAZFRG010000625          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFRG010000625 DAZFRG010000000
VERSION     DAZFRG010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.628"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..597)
                     /locus_tag="ACHKUC_005312"
     CDS             complement(<1..597)
                     /locus_tag="ACHKUC_005312"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HFO6621328.1"
                     /translation="MTGSAHAPSGDKSQWKACVRVNSFSKELSLSGVRYLQYERNRWQ
                     MSLPDKIINVPLRYELAYGGIWQPDGMEKLVLSANPVGCGYYPDISQLNTSCQYKLPQ
                     ITSSALSENATIFNGESDFFQGVGPVSRWWKSRLQYAGTYNEVWREKRYPYLPDDFDE
                     RFYNSAHPDMIYTGFLSGDENISLEGFFKFEQVVKTKLP"
BASE COUNT          193 a          137 c          113 g          168 t
ORIGIN      
        1 tggcaatttt gtcttaacaa cctgttcaaa tttaaaaaaa ccttcaagag agatgttttc
       61 atcgccagag agaaaccccg tgtatatcat atccggatga gcactattgt agaaccgctc
      121 atcaaaatca tccggcaaat agggatagcg cttttcacgc catacttcat tgtaggttcc
      181 tgcatactgg aggcgacttt tccaccatcg agatacaggg ccaacacctt gaaaaaagtc
      241 agattcccca ttaaaaatgg tagcgttttc tgacaaagca gagctggtta tttgtgggag
      301 tttatattga caacttgtat taagttgaga aatatcagga taatatccac aacctacagg
      361 gtttgctgaa agaaccaatt tctccatacc atctggttgc caaataccac cgtaagcaag
      421 ctcatagcgt agaggaacat taataatttt atctggtaag ctcatctgcc aacgatttcg
      481 ctcatattgt aagtagcgga caccagataa actcagttct ttagaaaaac tattaaccct
      541 cacacaagcc ttccactgag acttatcccc tgaaggggca tgagcactac ctgtcacgtg
      601 aatatctgta t
//
LOCUS       DAZFRG010000626          610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFRG010000626 DAZFRG010000000
VERSION     DAZFRG010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.629"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..66)
                     /locus_tag="ACHKUC_005313"
                     /pseudo
     CDS             complement(1..66)
                     /locus_tag="ACHKUC_005313"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            126..257
                     /locus_tag="ACHKUC_005314"
                     /pseudo
     CDS             126..257
                     /locus_tag="ACHKUC_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            302..406
                     /locus_tag="ACHKUC_005315"
                     /pseudo
     CDS             302..406
                     /locus_tag="ACHKUC_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          201 a          123 c          120 g          166 t
ORIGIN      
        1 cctcccgaac tcaaagctat catacagata attactggtt aaccctatat agggcatttt
       61 aaagtgttgt gatcttaccc agcaatagtg gacacgcggc taagtgagta aactctcagt
      121 cagaggtgac tcacaagaca aaacagtttc aaccagtaaa aaaacgtaaa caccattcgc
      181 atgaatttcg tagttaagat ctgaagcttg cagaatgcat cggtgttact gccggagtcc
      241 gtgaacttag tctgcattaa tcaccacagc tttacatctg tcgcagtaaa cagcaaaatc
      301 agcaaactta cttaattata tcgagtgcga ttacaatcgc tggcgatggt acagttggtg
      361 tggcggtctc agtccggaac aatttgaaaa tcagaatctc acttaaagcg gcatccattt
      421 tacaaaagat ggtcgtctgt gtatttgtaa tgaatacctt ctacatgttt agagtgcata
      481 atccacgaca aggcgatatc ctaaaaatta gctagcaggc aactttgatg gcatagataa
      541 tgtttttagt aaaaaagagt tgactgaata taatacagcc tcttcctaca taagaaggaa
      601 tgtgctcaca
//
LOCUS       DAZFRG010000627          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFRG010000627 DAZFRG010000000
VERSION     DAZFRG010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.630"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>608)
                     /locus_tag="ACHKUC_005316"
     CDS             complement(<1..>608)
                     /locus_tag="ACHKUC_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO6621329.1"
                     /translation="IILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGY
                     FFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRA
                     NDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGK
                     KCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISAND"
BASE COUNT          190 a          129 c          110 g          179 t
ORIGIN      
        1 tcatcattag cgctgatgtg atcaagatac tcgttcagat atcgccagtt taaatcgaca
       61 tgaattttat ctaccgctaa caaatcatga tctttgccgt tgatgtgtag cgtaaaagag
      121 atacattttt tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag
      181 tcatctgaag ccatcgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc
      241 cctgactcgt cgacaagcgg tccatttact tgttcctgat gataaacaac gattttatca
      301 ttcgccctcc gcgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct
      361 aaaaatggca tcatttccat tttcagaatc acggcacgtt gaatctcctc tgtatttttt
      421 aataaataca gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag
      481 ccaacgtttt tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat
      541 accagttgat agttaatgat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat
      601 aaaattat
//
LOCUS       DAZFRG010000628          606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFRG010000628 DAZFRG010000000
VERSION     DAZFRG010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.631"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..257)
                     /locus_tag="ACHKUC_005317"
     CDS             complement(<1..257)
                     /locus_tag="ACHKUC_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6621330.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQA"
     gene            complement(292..>606)
                     /locus_tag="ACHKUC_005318"
     CDS             complement(292..>606)
                     /locus_tag="ACHKUC_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6621331.1"
                     /translation="NEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLLVPLA
                     PLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGTEKLS
                     EA"
BASE COUNT          181 a          152 c          131 g          142 t
ORIGIN      
        1 gcctggtttg caactaccga gtcggcaata aagcattgcg gaccaatcgt tgcttccgct
       61 tcaataaccg catttttaat ttcggtggca aaaccaattt ttacgccatt gctgattatt
      121 gtgccaggac gaataaacgc ataattacct atcaggcagt ttgcgccaat cactaccggc
      181 ccctgaataa cggcaccgtg gcaaatacgg gtattcgcgc cgataacaac ctcaccggca
      241 ctttcatcaa taatcacctg gtctgcaatc tgaacatttt tctctttagc attaagcttc
      301 agatagtttt tcagttcctg tgacagaagg ttgggaataa gcggttgacg cttccaccag
      361 tgcggaatag cgggtgggtt tgtcgcgctg aggttcgcgt ccagtaaaaa aggcaataag
      421 tccataaata atccagatgg cataagctaa taatacatac acaacggaaa gcggtgctaa
      481 aggcaccaac aaccagcaac gatgaaacca ggcgctaaat gcaccaataa cacaaactac
      541 gccgacccag ataagcggga acattgccaa caccacacca tgtggcccgg tggtgatgaa
      601 ttcatt
//
LOCUS       DAZFRG010000629          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFRG010000629 DAZFRG010000000
VERSION     DAZFRG010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.632"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..602
                     /gene="lpxT"
                     /locus_tag="ACHKUC_005319"
     CDS             <1..602
                     /gene="lpxT"
                     /locus_tag="ACHKUC_005319"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO6621332.1"
                     /translation="FYFFNQKLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLK
                     ENAPGRRRIVIIGLVMLLTAVVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKD
                     ASRDSFPGDHGMMLLIFSAFMWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVG
                     SMTVILIGLPWVLLTPLSDRLITFFDKSLPGKNKHFQNK"
BASE COUNT          144 a          134 c          135 g          191 t
ORIGIN      
        1 ttttttattt ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat
       61 tgaccaacaa tcgcgctttc gacggttgtt cactgctggc gatgggaatg ttgatgctga
      121 gtttctggct gaaagaaaac gccccaggca gacgacgtat cgtgattatt ggtctggtca
      181 tgctattaac tgcagtggta ttaaaccagc tgggtcaggc actaattcct gtaaaacggg
      241 ccagcccaac gttgactttt accaatatta accgcgtcag cgaactgctc tctgttccca
      301 cgaaagatgc ctcacgagat agctttcccg gcgatcacgg tatgatgctg cttatttttt
      361 cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg
      421 tggtttttgc gtttcccaga gtaatgattg gcgcacactg gtttactgac atcatcgtcg
      481 gttccatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc
      541 gattaatcac cttttttgac aaatcacttc ctggaaaaaa caaacatttc caaaacaaat
      601 aact
//
LOCUS       DAZFRG010000630          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFRG010000630 DAZFRG010000000
VERSION     DAZFRG010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.633"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>594)
                     /locus_tag="ACHKUC_005320"
     CDS             complement(<1..>594)
                     /locus_tag="ACHKUC_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HFO6621333.1"
                     /translation="YGELFTVVVVNQTFDFSYSGGLCLIADEQRLPLMTDSWFGEPES
                     SSLKTATDLVCRKVRADVLLNGHAWHATGETTRWQASLQVGNLQRQFDICGPCQWQYI
                     SNRWQLSEPELTDRVLLRHELASASEFNPVGIVIPSLITPGDKSPCPAPQLSASPGAI
                     CRSWPSRLRYARGFTEQWQKTVQPFYPDEFDFAFMNYS"
BASE COUNT          167 a          141 c          136 g          150 t
ORIGIN      
        1 agaataattc atgaatgcaa aatcgaattc atctggataa aaaggctgaa cagtcttttg
       61 ccactgttct gtaaaacctc tggcatagcg caatcgtgaa ggccagctac ggcaaatagc
      121 tcctggtgag gcagataact gtggagctgg acagggcgat ttatcgccag gcgttatcag
      181 ggagggaata acaatgccta caggattaaa ttcactggct gacgccagtt catggcgtaa
      241 cagtacccga tcagttaatt caggttcgct aagttgccag cggttagata tatattgcca
      301 ttggcaaggg ccacatatat caaattggcg ctgaagatta cccacctgta aactggcctg
      361 ccatcgagtg gtttccccgg tggcatgcca tgcgtgtcca tttaataaaa catctgccct
      421 gacttttctg caaaccaaat cggttgctgt tttaagactg ctgctttccg gttcgccgaa
      481 ccaggagtcg gtcatcagtg gcagtcgctg ctcatccgca atcagacaaa gaccgccgct
      541 atatgaaaaa tcaaatgtct gattaactac cactactgtg aataactcac cata
//
LOCUS       DAZFRG010000631          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFRG010000631 DAZFRG010000000
VERSION     DAZFRG010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.634"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..426
                     /locus_tag="ACHKUC_005321"
     CDS             <1..426
                     /locus_tag="ACHKUC_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6621334.1"
                     /translation="KNWYEEVSNFQEDTIKIVYDENNIIVGITRDASTFNPEGFSVVE
                     VPDITANRRADDSGKWMFKDGAVIKRIYTADEQQQQAESQKAALLSEAESVIQPLERA
                     VRLNMATDEERSRLEAWERYSVLVSRVDPANPEWPEMPQ"
BASE COUNT          177 a          120 c          147 g          144 t
ORIGIN      
        1 aaaaactggt atgaggaagt aagtaatttt caggaagaca cgataaagat tgtttacgac
       61 gagaataata taattgtcgg catcaccaga gatgcttcaa cgttcaaccc tgaaggtttt
      121 agcgttgtcg aggttcctga tattaccgcc aaccgacgtg ctgatgactc aggtaaatgg
      181 atgtttaagg atggtgccgt gattaagcgg atttatacgg cagacgaaca gcaacaacag
      241 gcagaatcac aaaaggccgc gttactttcc gaagcggaaa gcgttattca gccactggag
      301 cgcgctgtca ggctgaacat ggcaacagat gaggagcgta gccgactgga agcatgggaa
      361 cgctacagtg ttctggtcag ccgtgtggat cctgcaaatc ctgaatggcc ggaaatgccg
      421 caataagttg tatgagctga catatctata gcacagagta aagcctaatc tgacagtccg
      481 ctctgtgcaa ggagcggaca ttacctaaca cattggtaac cttcacagat ttacgcgttc
      541 tacatagtta gcattgtatc tgatgagtta tcatgtttta aaatatcc
//
LOCUS       DAZFRG010000632          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFRG010000632 DAZFRG010000000
VERSION     DAZFRG010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.635"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>585)
                     /locus_tag="ACHKUC_005322"
     CDS             complement(<1..>585)
                     /locus_tag="ACHKUC_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EvgS"
                     /protein_id="HFO6621335.1"
                     /translation="LNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIIN
                     KALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLL
                     RSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKN
                     AMLPLENSESPFKDVFSNTHEVTAETKENRTIYT"
BASE COUNT          179 a          101 c          122 g          183 t
ORIGIN      
        1 gtgtgtatat tgttcggttt tctttcgttt ctgctgtgac ttcatgtgta ttagaaaaaa
       61 catctttaaa gggggattca ctgttttcta atggtaacat tgcattttta tagtaatcag
      121 cagtgaaata atgttcaaat gcactattgt gactaatgac attaccttgc cagtttacaa
      181 cataagttgg attcggtaag gaatccgaga gcgctttccg gaatgatatt tggttttcta
      241 aatcaccctg aataacttta cgacgacgaa ctgagcgtaa caggtagaat ccccataaaa
      301 ggctactgcc aactaataaa acggataatg tcgtaacaat ataaaattgc tcgctataga
      361 ggtcccatgt gtcaatggtc acattgggca ttttaatcca tttttcggtc aggcgcagaa
      421 cttcgcttgg gggaattgca ttcagtgctt tattaataat atccttaagt tccggttctc
      481 cgcgaggaaa agcaaacgaa agtgatgcat tctgaacgcc gggaataaga aaatgataaa
      541 gttcattagg atagtaatgg tcgatcatgt aacgcgaatt taact
//
LOCUS       DAZFRG010000633          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFRG010000633 DAZFRG010000000
VERSION     DAZFRG010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.636"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..516
                     /locus_tag="ACHKUC_005323"
     CDS             31..516
                     /locus_tag="ACHKUC_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621336.1"
                     /translation="MKKKLIAAAVLFMTANMANAATLDNTSSVKADFEVVGGCTVSGT
                     WTGEQVPAAVYQGSAKRVGVLGISLSGCTSQVYFEGTDKNSDGLPQATDPKGNKIIIV
                     PSFNTLNTLVSKDATLNVFYSKNSLNDGDTLSVELVNRDQWNAKAGKHTMTLNVGTYS
                     I"
BASE COUNT          162 a          115 c          128 g          180 t
ORIGIN      
        1 ttgtgttttt aatcctttaa aggtgttaac atgaaaaaga aacttattgc agctgcagtt
       61 ctgtttatga cggctaacat ggcgaatgct gcaactctgg ataatacatc ttccgtaaaa
      121 gctgactttg aggttgtcgg tggttgcact gtctctggaa catggactgg tgagcaagtc
      181 cctgctgctg tatatcaggg ctcagctaag cgtgtaggtg tacttggtat ttcgttgtct
      241 ggttgcacta gccaagttta tttcgaaggg accgataaaa actcagacgg actgcctcaa
      301 gccactgatc ctaagggtaa taaaataatt attgttccat cattcaatac attaaatact
      361 cttgtgtcta aagacgctac acttaatgtg ttctatagca aaaattccct gaatgatggt
      421 gacactcttt ctgttgaatt agttaaccgt gatcagtgga atgctaaagc tggtaaacat
      481 actatgactc ttaatgtagg tacttactct atctaataca gttagtcccc ggcatagcgc
      541 cgggggcatt attgtgcaca gaaaaccccc agctaggctg taatg
//
LOCUS       DAZFRG010000634          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFRG010000634 DAZFRG010000000
VERSION     DAZFRG010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.637"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..261)
                     /locus_tag="ACHKUC_005324"
     CDS             complement(<1..261)
                     /locus_tag="ACHKUC_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621337.1"
                     /translation="MKIFKIGDIHPSVPHLFADLAELATVINYSGRYDLHKNDLITIR
                     TQSNTSVDDIDQEYQEDENEGCDAERNDRLERQVEDVWTQLDF"
     gene            complement(248..>579)
                     /locus_tag="ACHKUC_005325"
     CDS             complement(248..>579)
                     /locus_tag="ACHKUC_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO6621338.1"
                     /translation="YKKDDSGKTKVGESRNLRLLSSVIDNPKALTSFRNGADLKVAYQ
                     LTEDLKQDFMTLLYKAESALIEAAGIVATIDYNPEALEVARRLSQNVKLIGNTIKAKK
                     VSDDEDF"
BASE COUNT          167 a          115 c           98 g          199 t
ORIGIN      
        1 aaaatcgagt tgcgtccaga catcttcaac ttgtctttct aaacggtcat tgcgttcagc
       61 atcgcagcct tcattttcat cttcttgata ttcttgatca atatcatcca cacttgtgtt
      121 actttgagtt cttattgtta tgaggtcatt tttatgtaga tcataacgac cagaataatt
      181 tataaccgta gcaagttctg caaggtctgc aaaaagatga ggaacactag gatgaatgtc
      241 tccgatttta aaaatcttca tcgtcagaaa cctttttggc tttaattgta ttaccaataa
      301 gtttgacatt ttgacttagg cgtcttgcaa cttccaatgc ttcaggattg taatcaatgg
      361 ttgcaacaat tccagccgct tcaattaatg cagattcagc tttataaagt aacgtcatga
      421 aatcttgttt taagtcttca gttaactgat acgcaacctt aaggtcagcc ccgtttctaa
      481 aggacgttaa agccttgggg ttatcaataa cagaagataa taggcgtaaa tttcgggact
      541 ctccaacctt tgttttcccg gaatcatcct ttttataaa
//
LOCUS       DAZFRG010000635          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFRG010000635 DAZFRG010000000
VERSION     DAZFRG010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.638"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>577
                     /locus_tag="ACHKUC_005326"
     rRNA            <1..>577
                     /locus_tag="ACHKUC_005326"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          125 c          177 g          113 t
ORIGIN      
        1 taatgggtca gcgacttata ttctgtagca aggttaaccg aataggggag ccgaagggaa
       61 accgagtctt aactgggcgt taagttgcag ggtatagacc cgaaacccgg tgatctagcc
      121 atgggcaggt tgaaggttgg gtaacactaa ctggaggacc gaaccgacta atgttgaaaa
      181 attagcggat gacttgtggc tgggggtgaa aggccaatca aaccgggaga tagctggttc
      241 tccccgaaag ctatttaggt agcgcctcgt gaattcatct ccgggggtag agcactgttt
      301 cggcaagggg gtcatcccga cttaccaacc cgatgcaaac tgcgaatacc ggagaatgtt
      361 atcacgggag acacacggcg ggtgctaacg tccgtcgtga agagggaaac aacccagacc
      421 gccagctaag gtcccaaagt catggttaag tgggaaacga tgtgggaagg cccagacagc
      481 caggatgttg gcttagaagc agccatcatt taaagaaagc gtaatagctc actggtcgag
      541 tcggcctgcg cggaagatgt aacggggcta aaccatg
//
LOCUS       DAZFRG010000636          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFRG010000636 DAZFRG010000000
VERSION     DAZFRG010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.639"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          178 a          109 c          109 g          180 t
ORIGIN      
        1 ctggaaatat aggggcaaat ccacgaggtg tcaaagcaca cgtttggatt gtggcatcct
       61 tgcttatccg taaggctttt accgggtcac agttcaccat gccggactga tgatctacac
      121 gcttagagtt acataccaaa gtaaaaccat agatgaatgc aaacctgcat cgtcaggagg
      181 ttaaatttca gacctgtcag ttaccgcgta aatggtttga tatatgacta atttatttgc
      241 gcaggatttt tacgacaacc ctctgaaaca gaagttgacg ggatatatag ttgaactaat
      301 tacagataaa gcttttctgt atctctggcg gtggttattg atcagtaatc gttattggtt
      361 gttctatatt attctgtatg acggcacaac gtctttaaca actggcgctg tcacaacata
      421 agtccccaaa aaatattata taagtaacaa cagtctatat aggagtgtaa attacttact
      481 atcaaatact tactccaata gtatttctgt cgattctatt ccaattcttt aatagaaaga
      541 atgaggttgg ataatatatg agcacctttc cctatc
//
LOCUS       DAZFRG010000637          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFRG010000637 DAZFRG010000000
VERSION     DAZFRG010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.640"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..256
                     /locus_tag="ACHKUC_005327"
     CDS             <1..256
                     /locus_tag="ACHKUC_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621339.1"
                     /translation="HFGPDMERFESCFEAALFAIGEWQIKVDAMTRYHEEDEVMEFFS
                     SIPSDEAGNLATSILLFSDVLAMKGVDEVFGYFLGKDNVV"
     gene            253..558
                     /locus_tag="ACHKUC_005328"
     CDS             253..558
                     /locus_tag="ACHKUC_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO6621340.1"
                     /translation="MSDESKKPRTEKTLKQKVAFAQLELNRLKSMEKSERKKVETRLK
                     IILGAEVAKAMHCDIEQVDKELVMGILLSVSDLNDIERIKYIKAGRWFLAQMDGRQK"
BASE COUNT          192 a           78 c          142 g          164 t
ORIGIN      
        1 tcattttggc cctgatatgg aacgatttga gtcctgcttt gaagcagcgc tttttgctat
       61 tggtgaatgg caaataaaag ttgatgcgat gacacgatac catgaggaag atgaagtgat
      121 ggagttcttt agctctatac catcagatga agcgggaaat ctggcgacgt ccattttgct
      181 gttcagtgat gttcttgcca tgaaaggcgt ggatgaggta tttggctatt ttttgggtaa
      241 ggataatgtt gtatgagtga tgaaagtaaa aaaccaagaa cagaaaaaac attaaagcag
      301 aaagtagcat ttgctcaact tgagcttaac cgacttaagt caatggagaa atcagaacga
      361 aagaaagttg aaacccggct aaagattatt cttggtgcgg aagtagctaa ggctatgcat
      421 tgcgatatcg aacaggtgga taaggaactt gtcatgggga ttttgctttc agtttctgac
      481 ctgaatgata ttgaaagaat aaagtatata aaagcaggga gatggtttct tgctcaaatg
      541 gatggaagac aaaaataaaa aaactaaatt tatttt
//
LOCUS       DAZFRG010000638          573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFRG010000638 DAZFRG010000000
VERSION     DAZFRG010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.641"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>573
                     /locus_tag="ACHKUC_005329"
     CDS             <1..>573
                     /locus_tag="ACHKUC_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFO6621341.1"
                     /translation="FINKTRVVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQ
                     ENYKDDAFEKGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKE
                     ADKSGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMAEDSFPRA
                     LCDYNEMVDEIFQLIVIAGRVCDEHGYVTKS"
BASE COUNT          182 a          109 c          145 g          137 t
ORIGIN      
        1 tttataaaca aaacgcgtgt agtggcgtta acgcctgcgc tgcaacctat tgatggcgtg
       61 gcggtgagtt atattgatgc tgccgtagcg ttgggtaata ccattaacga aatggataaa
      121 tattacaccc aggaaaatta taaagacgat gcgtttgaga aagggaaaac attccactac
      181 tactctctgg cagtcatgat ttcagcgaaa caaattaata acctgatatc gcaagataag
      241 tttgatgcag aagcggcaat gaagaaagtg tctgaactgg aaacgctggt ggcgcaggcc
      301 aaagaagcgg ataaaagcgg catgaatttc tcgtttatta attcggcagg ccagtatcaa
      361 ctcgaggcta aaaaatacgt tcgccgcatc agagataaag tcccgtactc tgactgggat
      421 aaagagcaac ttcaggacgc aaactcaagc tggatggccg aagactcatt tccacgagcg
      481 ttatgcgatt acaacgaaat ggtcgatgaa atatttcagt taattgtaat cgcggggcgg
      541 gtttgtgatg agcacggata tgtcacgaag tcg
//
LOCUS       DAZFRG010000639          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFRG010000639 DAZFRG010000000
VERSION     DAZFRG010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.642"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            228..398
                     /locus_tag="ACHKUC_005330"
                     /pseudo
     CDS             228..398
                     /locus_tag="ACHKUC_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          163 a          144 c          124 g          136 t
ORIGIN      
        1 tccgattccg ggcaccacta attcttaaga acccgcccac aaggcgggtt tttgcttttg
       61 gatctttata taaaataaac ttgggtgcat aacgccatca ggtcttaaac actcccgaat
      121 catcaataaa catcccccgc taacaaaaca cagttcagtt cacataacat ccctgttatc
      181 ccacacatat ctgctggccg aacgacgcca gtgcgcgtac ggaggcaaga gcagccctgc
      241 acatggcagc gcttgtggta acctggttta atccggtgaa agaggcgttt tacatgcatc
      301 tgccagcagc cggaaaaacc aaaaaagtgg cgctggttga ctgtatgcgt aaattgctga
      361 ccatcctgaa cgcaatgctc agaaagaacg aagagtgaaa tgaatcgtag catcatgttg
      421 ctccataatt tttacgttca agacagttga ttatcggcgc atatgactca aaaagagcct
      481 gatgattgcc tcttttccat aggctctttc tctgaagtga aggaaggccc ggaagaaagc
      541 actcgctggc atacgtgact ccagctc
//
LOCUS       DAZFRG010000640          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFRG010000640 DAZFRG010000000
VERSION     DAZFRG010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.643"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..232
                     /locus_tag="ACHKUC_005331"
     CDS             14..232
                     /locus_tag="ACHKUC_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6621342.1"
                     /translation="MKENSETSVKRFIRVPDVLNRVGFSRTTLYERIKEGNFPDRVKI
                     GPRCVAFVESEIDEWIEAAIRHSRQNAA"
     gene            314..>560
                     /locus_tag="ACHKUC_005332"
     CDS             314..>560
                     /locus_tag="ACHKUC_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621343.1"
                     /translation="MKIELITTKQFIEQAECYFRSYMDGLRRNAPDDFYYFLNNKYNM
                     NDIMESIIKKTRCHFYDDTEEDQRNRIYGEVSHCKVKQ"
BASE COUNT          211 a           84 c          121 g          144 t
ORIGIN      
        1 tagaggacag acaatgaaag aaaatagtga aaccagcgta aaacgattta tccgcgttcc
       61 tgatgtttta aatcgagtgg gatttagtcg cacgacttta tatgaacgta ttaaggaagg
      121 aaactttcca gacagagtaa aaataggtcc gaggtgtgtc gcttttgttg aaagtgagat
      181 tgatgaatgg attgaagcgg ctattcgaca ctcacgccag aacgcagcat aaaaacagaa
      241 acaagatctc gtgtcgaaca gatatctatt cgctcagggc atcttattat tctaaacaat
      301 aatggagaaa aatatgaaga tagagcttat tacgacaaaa cagtttattg aacaagccga
      361 atgttatttc aggagctata tggacggatt gcggaggaat gcaccagatg atttttatta
      421 ttttctcaac aataaataca atatgaatga cattatggaa agtatcatta aaaaaacgag
      481 atgtcatttt tacgatgaca cagaagaaga ccaaagaaat cggatttatg gagaggtaag
      541 ccactgtaag gtaaaacaac
//
LOCUS       DAZFRG010000641          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFRG010000641 DAZFRG010000000
VERSION     DAZFRG010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.644"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..330
                     /gene="wzy"
                     /locus_tag="ACHKUC_005333"
     CDS             <1..330
                     /gene="wzy"
                     /locus_tag="ACHKUC_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085454979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O18ab/O18ac family O-antigen polymerase"
                     /protein_id="HFO6621344.1"
                     /translation="ELPVNTILDYSYVPVPTNVYTALQPFYQDFGYTGIIFGAVLYGL
                     IYVSLYTAGVRGNNTQALLIYALFSVSSATAFFAETLVTNLAGNVKLVLCTILLWRFT
                     VICKPAQ"
     gene            339..>551
                     /locus_tag="ACHKUC_005334"
     CDS             339..>551
                     /locus_tag="ACHKUC_005334"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6621345.1"
                     /translation="MATYNGEAFIKNQILSLQQQTFSNWRLFIQDDGSTDNTISIIKN
                     FQKSDSRIRLVDDNLKGQGAGKNFLSL"
BASE COUNT          177 a          102 c           99 g          173 t
ORIGIN      
        1 gaattgccag taaatactat tcttgactat tcatacgttc ctgtaccaac aaatgtatat
       61 actgcacttc aaccatttta ccaggatttt ggttatactg gcatcatatt tggagcagta
      121 ttatacggac taatatatgt gagtttatac acggccggtg ttcgtggaaa taatacacag
      181 gcattactga tttacgcatt gttttcagtt agcagtgcaa cggctttctt cgctgaaacg
      241 ctagtaacga atttagctgg aaatgtgaag ttagtattat gtaccatctt actatggcga
      301 tttacagtaa tatgcaaacc agcacagtaa ccattctaat ggccacctac aatggcgagg
      361 ccttcatcaa aaatcagatt ttgtcactac aacaacaaac attttctaac tggcggttat
      421 ttattcagga tgatgggtct acagacaata ctatatctat aataaaaaac ttccaaaaat
      481 ctgactccag aattcggcta gttgatgata atttgaaagg tcaaggtgca ggaaaaaatt
      541 ttttatcgct g
//
LOCUS       DAZFRG010000642          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFRG010000642 DAZFRG010000000
VERSION     DAZFRG010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.645"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..378
                     /locus_tag="ACHKUC_005335"
                     /pseudo
     CDS             112..378
                     /locus_tag="ACHKUC_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          145 a          131 c          112 g          162 t
ORIGIN      
        1 ccagaacata aagcctctcc tctttcatga ttggcctgtg cataccggat ataggctggc
       61 tggtatgcag gtttgccgtt tgccgcatct tccctgcgtt gaagagtcag gctggcaaac
      121 cgcgtgcaaa tgaacacccc ctcttttcat caacacttca gacagcttgc gggaatgagt
      181 ccactccggt atcagaaatg gctacgcctc aatgaagcca ggcgactgat gctgaatgaa
      241 cattacgatg tcaccactgc agcctatgtt gtcggttacg aaagcttatc ccatttcagt
      301 cgggaatatt cgcggatgtt tggagagtca cctaagagag atattaccag gttaaaaaag
      361 tctgtcggtc agctttaata aaccctgcac ttatctgttt agagcaattc tgtgttagtt
      421 tcaccaggca aatattaacc atagctaatg tttatagcca tataaactat tagtacctct
      481 tgacctttcc tgacacttcc cttgttcgca ataatattct gtttagtcgc tgtttataaa
      541 taacatgcat
//
LOCUS       DAZFRG010000643          548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFRG010000643 DAZFRG010000000
VERSION     DAZFRG010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.646"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..509
                     /locus_tag="ACHKUC_005336"
     CDS             <1..509
                     /locus_tag="ACHKUC_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO6621346.1"
                     /translation="DLSPSGPLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIM
                     TSAVVISSWYTDEYKKLFALPDESELQLQDARELLDWLIEECRGECPPRVRKNYILQC
                     GPGRFRNRKKLNVLLNILASQLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIR
                     WHEQSFGL"
BASE COUNT          148 a          122 c          137 g          141 t
ORIGIN      
        1 aggatttgtc tccttccgga cccttaagcc agtttcgggg gcatgtttca aaaaaaacag
       61 aaaacattgc gagaattgct gcgttattgc agtatttcag tcagggtgaa gggaaaatct
      121 ctgccgatat catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa
      181 aattatttgc gttacctgat gaatctgaac tgcagctgca ggacgcccgg gagctgctcg
      241 actggctcat tgaggagtgc cgcggagaat gcccgccccg tgtcaggaaa aattatattc
      301 tccagtgtgg cccgggccgg ttcaggaaca ggaaaaagct gaacgtcctg ctgaacattc
      361 ttgcatctca actcaggctc tctgtcgtgc aggaaggaaa aaccacctat gtcctgcttt
      421 ccgaaataaa caatatcacg ctgcctgaac tgaatgcgtg ctacagccag aaactgataa
      481 gatggcatga gcaatccttt ggcttataat gttccctgtt gctgagacgg attattcagt
      541 ggtggtat
//
LOCUS       DAZFRG010000644          548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFRG010000644 DAZFRG010000000
VERSION     DAZFRG010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.647"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>548
                     /locus_tag="ACHKUC_005337"
     CDS             <1..>548
                     /locus_tag="ACHKUC_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621347.1"
                     /translation="LVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEE"
BASE COUNT          123 a          128 c          166 g          131 t
ORIGIN      
        1 cgctggtgac atctctggca ctggtagctg tcacaaccgg ccctgacatg atcaaaatgt
       61 tgcggggacg gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttccggtg
      121 tggcaggtgg tgctgtcggg tcagtggcgg gcgggatatt attcagtcca ctgggaccat
      181 ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg
      241 tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga
      301 ttcaggagca ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa
      361 atctgaacgc gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg
      421 ccggtataca acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca
      481 tcaggcaacg ccccgtcatg gaatatgatg catcccatct cggcaatatg gttaaccgac
      541 tggaagaa
//
LOCUS       DAZFRG010000645          548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFRG010000645 DAZFRG010000000
VERSION     DAZFRG010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.648"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>548
                     /locus_tag="ACHKUC_005338"
     CDS             <1..>548
                     /locus_tag="ACHKUC_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621348.1"
                     /translation="LVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSCVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QMTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEE"
BASE COUNT          123 a          129 c          165 g          131 t
ORIGIN      
        1 cgctggtgac atctctggca ctggtagctg tcacaaccgg ccctgacatg atcaaaatgt
       61 tgcggggacg gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttcctgtg
      121 tggcaggtgg tgctgtcggg tcagtggcgg gcgggatatt gttcagtcca ctgggaccat
      181 ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg
      241 tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga
      301 ttcaggagca gatgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa
      361 atctgaacgc gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg
      421 ccggtataca acaacgggcc gcgaccaaca tgttaatcaa accactggtg gtcagtatca
      481 tcaggcaacg ccccgtcatg gaatatgatg catcccatct cggcaatatg gttaaccgac
      541 tggaagaa
//
LOCUS       DAZFRG010000646          534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFRG010000646 DAZFRG010000000
VERSION     DAZFRG010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.649"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..185)
                     /locus_tag="ACHKUC_005339"
     CDS             complement(<1..185)
                     /locus_tag="ACHKUC_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFO6621349.1"
                     /translation="MDSCHKIDYGLYALEILAQYHNVSVNPEEIKHRFDTDGTGLGLT
                     SWLLAAKSLELKVKQVK"
     gene            complement(256..>534)
                     /locus_tag="ACHKUC_005340"
     CDS             complement(256..>534)
                     /locus_tag="ACHKUC_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621350.1"
                     /translation="KDGRVITPDSLKKAFEYQQSNNKVSYVYGHDASTYGSQDNLNPL
                     INEISKIISAAGNFDVKEERSAASLLQLSGNASDFSYGRNSITLTASA"
BASE COUNT          157 a          110 c           89 g          178 t
ORIGIN      
        1 ttttttacct gttttacctt tagttctaaa gatttcgcag caagcaacca tgacgttaat
       61 cccagaccag tcccgtctgt gtcaaatcta tgtttaattt cttccgggtt aacagagacg
      121 ttatggtatt gggctaaaat ctccagggcg tataacccat aatcaatttt atgacaagaa
      181 tccattatga ctccaaaaaa atagcaatct tatgtggcac agcccagtaa gattgctatc
      241 atttaaatta atatattatg ctgatgctgt caaagttatt gagttccgtc catatgaaaa
      301 atcactggca ttaccggaca actgcaataa agaagcggca gatctttcct ccttaacatc
      361 gaagttacct gcagctgaaa tgattttgct gatttcatta attaatggat taagattgtc
      421 ctggctccca taagttgatg catcatgccc atacacataa cttaccttgt tattactctg
      481 ctgatattca aatgcttttt taagagaatc tggtgtgatt accctgccgt cttt
//
LOCUS       DAZFRG010000647          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFRG010000647 DAZFRG010000000
VERSION     DAZFRG010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.650"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..460)
                     /locus_tag="ACHKUC_005341"
     CDS             complement(<1..460)
                     /locus_tag="ACHKUC_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO6621351.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKD"
BASE COUNT          155 a          105 c          102 g          170 t
ORIGIN      
        1 gatctttaca ttcgaataag taacgaacgc ctttcttgcc tgatttatag gtgagtattt
       61 tgtatccatc gtaatcatat ttacaggatg ttgtttcatt atttctatgg aattcgacaa
      121 tgccgtcctc aatgccaatc atttcgatat ctgtcgcata acctttgcga taataatctt
      181 taatttcagc aaatgttttg gttttatctg catccgcttt cttctgaaag acggggtcaa
      241 gtttgccact ttgcagtaaa gggtaaacgg attgccagac tccatcccag tcactgagcg
      301 ttcggttttg tacattggta tcatcaaaaa caccattagc agctttttgt tcgacctctg
      361 ttaagggttt gccgtgtgag tgatggccat gcgaaaaggc aggagcgcta acaataaaga
      421 cacctaaagc aacagccagt ttatgaagac gaatcgccaa aacaatttcc tccagagtta
      481 atgtacatga tgtgtaatgt tatattgtaa catatgaaaa aatgcaacaa ct
//
LOCUS       DAZFRG010000648          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZFRG010000648 DAZFRG010000000
VERSION     DAZFRG010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.651"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..296
                     /locus_tag="ACHKUC_005342"
     CDS             <1..296
                     /locus_tag="ACHKUC_005342"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6621352.1"
                     /translation="VCAAGMSTSMLVKRMIDHATAISLEVNISALAITEAKGKIKNNE
                     VDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQE"
     gene            411..>532
                     /locus_tag="ACHKUC_005343"
     CDS             411..>532
                     /locus_tag="ACHKUC_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6621353.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFP"
BASE COUNT          171 a           90 c          116 g          155 t
ORIGIN      
        1 tagtttgcgc tgcgggcatg tcaaccagta tgctggttaa acgtatgatt gatcatgcta
       61 ccgctatttc acttgaagtt aatatttccg cattggcgat tacagaggct aaagggaaaa
      121 ttaaaaataa cgaagttgac gttgttttac ttggtccgca agttcgcttt cagaaaccag
      181 agattgaagc cgttgcacag gggaaaatgc ctgtagccgt tatcgagatg aaagactatg
      241 gcacaatgaa cggacaggca gttcttgaat ttgcgatgaa actactgcaa gagtaagaat
      301 ctaatattat taaactatta cctgaactaa cttttcacac atcataaaat ggatatggca
      361 tacatgcttt attgggatgt ataaatatcc aggacaacag gtataaaaaa atgggattaa
      421 tggcatcatt cgaacgtgga atggaacgtt ttcttgttcc agttgctatc aagttaaact
      481 cacaaaaaca tgttgcagcg gtgagagatg gattcgtttt tacgtttcca at
//
LOCUS       DAZFRG010000649          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFRG010000649 DAZFRG010000000
VERSION     DAZFRG010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.652"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..277
                     /locus_tag="ACHKUC_005344"
     CDS             <1..277
                     /locus_tag="ACHKUC_005344"
                     /inference="COORDINATES: protein motif:HMM:NF044473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621354.1"
                     /translation="HFPAFTDTLWLEVSQYPELIAQFSSGNQTRIKQGVIAKAPLEGW
                     HYGTKEIVKKFHMYHRVAIEYSGGIYDIDNLRRAHLICMMKFITGDS"
     gene            278..>530
                     /locus_tag="ACHKUC_005345"
     CDS             278..>530
                     /locus_tag="ACHKUC_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO6621355.1"
                     /translation="MYNFKDEIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWR"
BASE COUNT          183 a           88 c          118 g          141 t
ORIGIN      
        1 tcattttccc gcttttaccg ataccctttg gctggaggtg agtcagtatc cggagttgat
       61 agcgcagttt tcaagcggta atcaaacacg tataaaacaa ggagtaatcg caaaagcacc
      121 acttgaaggt tggcattatg gcactaaaga aatcgttaaa aaattccaca tgtatcatcg
      181 tgttgcgatt gaatatagtg gcggaattta cgatattgat aatttaagga gggcacacct
      241 catttgcatg atgaaattta ttacaggaga tagttaaatg tataatttta aagatgaaat
      301 agaagattac acagagcggg aattcattga gttgcttggc gagtttacaa atccaaccgg
      361 ggataacgct caattaaaag gggaagagct tgacaaatat tgggatgatc tagaagaaca
      421 tcttaccaga attacacaac atccgttgat gagcgatctt atctactatc ctgcaaagaa
      481 gggagatgat gaacctgaaa acatcctgaa aattgtcaaa gaatggcggc
//
LOCUS       DAZFRG010000650          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFRG010000650 DAZFRG010000000
VERSION     DAZFRG010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.653"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            228..488
                     /locus_tag="ACHKUC_005346"
     CDS             228..488
                     /locus_tag="ACHKUC_005346"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6621356.1"
                     /translation="MVVLFSHIFHSHSTKYIANKLNISNKTVYSHIYHAMEKNLLCRY
                     SVKYLCYLQNNMKKTSTQCDGKLDSGKVRIKQGCRGSCKKLP"
BASE COUNT          174 a           76 c          102 g          176 t
ORIGIN      
        1 gccgtgattt gtttacactc gacaataaaa gagatatatt gaataatgac aatttcatta
       61 ttttctgtga cgagcagttt ataagattga tgagctgtat tttttgtggg cgccattttt
      121 ctataatatc aacaaataaa gtgaaatcca tctctgatat aataacgcac attcgtaggg
      181 gggtttattg ttatggtgat tcgttttgct ccctcagtat gagggaaatg gtggttctgt
      241 ttagtcatat ttttcatagt cacagcacaa agtatattgc taataaatta aatataagta
      301 ataaaacagt ctatagccac atctatcatg caatggagaa aaaccttctt tgcagatata
      361 gtgtaaaata tctttgttac cttcagaata atatgaaaaa aacatccact caatgtgatg
      421 gtaaactgga ctcgggaaaa gtcaggataa aacaaggatg tcgtggttcc tgtaaaaagc
      481 ttccttgagt tgtagggaga gattattatt cgttatgaag tcacctgt
//
LOCUS       DAZFRG010000651          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFRG010000651 DAZFRG010000000
VERSION     DAZFRG010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.654"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..273)
                     /locus_tag="ACHKUC_005347"
     CDS             complement(76..273)
                     /locus_tag="ACHKUC_005347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621357.1"
                     /translation="MAKPKYSLETRLAAVNHYLAGHDGARRIAERFGVEETSLRKRDK
                     FSPRPCHIYAPSIGGNFPVII"
     gene            complement(343..>528)
                     /locus_tag="ACHKUC_005348"
     CDS             complement(343..>528)
                     /locus_tag="ACHKUC_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621358.1"
                     /translation="AKFKDGSPDKLKDISPYQKIEDSNEEYDNLHDEEKVKLFEYLEK
                     EHGVENISPVKLRRPRP"
BASE COUNT          141 a           94 c          111 g          182 t
ORIGIN      
        1 tttgtaaaga gcgtaaataa tgagttattt ttgttctaaa gatatattta aaaaaggcac
       61 ttcgaaagaa atacatcaaa tgattacagg aaagttgcca cctattgatg gagcgtatat
      121 atggcatggt cgtgggctaa acttatctct tttgcgcaat gatgtttctt caacaccaaa
      181 acgttcagcg atacgacgtg cgccatcgtg tccggcaaga taatgattaa cggcagctaa
      241 tctggtttcg agagaatatt ttggctttgc cacaaaaaaa tgcaccttaa tcagttgggt
      301 gtccaacttt tggggcgcag tccattatgc tgaggcttat ttttaaggtc tcggtcttct
      361 tagcttaact ggagagatat tttctacacc atgctctttc tctaaatatt cgaataattt
      421 cactttttcc tcgtcgtgaa ggttgtcgta ttcctcatta gaatcttcaa ttttttggta
      481 tggggatatg tccttgagtt tatcgggtga gccgtctttg aatttggc
//
LOCUS       DAZFRG010000652          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFRG010000652 DAZFRG010000000
VERSION     DAZFRG010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.655"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          179 a          117 c           89 g          142 t
ORIGIN      
        1 aagctaagat tcactagtat aatgagtctg aacctctgca tcagagcaat cgcggcacac
       61 acaagtgagt aaactagcga cacgataaaa tccgtatcga ctatcataac cttccgtaca
      121 ccgctggagt tgaaaattat caggaaattc ttaacgtaca gaccctaccg aaaggactgt
      181 ctgtataaac cagaattcta tgcttataga aaaagctatg acatactttg caaaagtctt
      241 aaataaatta tggcctagca tgaaggtgcc aagggaacca taaaacttat cagtgatacg
      301 taccgatccc ctccgcatag cctcgtccat acagccccag acttttagcc tgactaagaa
      361 aattatccgc atttatctga ataatgacat ttacatagta gtatgtatgt gagccgctgt
      421 tgttacgcga ccatctatgc gcaaatgttt tccatacacc gaatttacta catgaaaaaa
      481 taacattcat cacaactatt tagattgata aagtgcgcct tatgttt
//
LOCUS       DAZFRG010000653          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFRG010000653 DAZFRG010000000
VERSION     DAZFRG010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.656"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>526
                     /locus_tag="ACHKUC_005349"
     CDS             <1..>526
                     /locus_tag="ACHKUC_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6621359.1"
                     /translation="LIESASQTTNIANISLAKLNPLPVCIPPAKEQIHIVKKMNELMS
                     LCDQLEQQSLTSLDAHQQLVETLLGTLTHSQNAEELAENWARISEHFDTLFTTEASVD
                     ALKQTILQLAVMGKLVLQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEE
                     KPFELPEGWEWCRL"
BASE COUNT          181 a          117 c          115 g          113 t
ORIGIN      
        1 agttaattga atcagcaagt caaactacaa atattgctaa tatatcgtta gcaaaactta
       61 acccattacc agtatgtatt cctccagcta aagagcaaat tcatattgtt aaaaagatga
      121 atgaactcat gtccctatgc gaccaactag aacagcaatc cctgaccagt ctggacgcac
      181 atcagcaact ggttgaaaca ctgttgggaa cgcttacaca cagccaaaac gccgaggaac
      241 tggctgaaaa ctgggcgcgt attagcgagc atttcgacac actatttacc accgaagcca
      301 gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggtaaa cttgtgctgc
      361 aggatccgaa tgacgaacca gcctctgaac tgctcaaacg aattgcgcag gaaaaagctc
      421 aattggtgaa agaagggaaa ataaaaaaac aaaaaccgtt gcccccgatt agcgatgagg
      481 aaaaaccgtt tgaacttccg gaagggtggg agtggtgtcg tttaaa
//
LOCUS       DAZFRG010000654          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZFRG010000654 DAZFRG010000000
VERSION     DAZFRG010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.657"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..242
                     /locus_tag="ACHKUC_005350"
     CDS             <1..242
                     /locus_tag="ACHKUC_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6621360.1"
                     /translation="GLEKMLIGMRVKSERSTCVVSNGKLSSETKTKWEYIRSTATIRI
                     GGQTTAGLRHLFGHVRNFRLWHKELTDAQLGEVVE"
     gene            239..517
                     /locus_tag="ACHKUC_005351"
     CDS             239..517
                     /locus_tag="ACHKUC_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621361.1"
                     /translation="MRDFTLRFSDKADFRAFLRKLNWEEDEELQNAVLVDEIGFTFRE
                     TDVSDDGEPEYTRNEGYFVNIRLLDDGFDDSVFREWVVTPERPLREWF"
BASE COUNT          143 a           90 c          150 g          134 t
ORIGIN      
        1 ccgggctgga aaaaatgctt attggtatgc gggtaaagtc cgaacggtcc acatgtgtag
       61 tcagtaacgg taagttaagc agcgaaacta aaaccaaatg ggaatatatc cggagtacag
      121 caaccattcg cattggtgga caaactacag caggattacg ccatttattt gggcatgtga
      181 ggaattttcg tctctggcat aaagagctaa cagatgcgca gcttggggag gttgtggagt
      241 gagagatttc acgttgcgtt tcagtgataa agcagatttc agggcatttc tcaggaaact
      301 taactgggaa gaggacgaag agctgcagaa tgccgttctg gttgatgaga ttggttttac
      361 gttcagggag acagatgttt ctgatgacgg agaaccagaa tacacgcgaa acgaagggta
      421 ctttgttaat atccgtcttc ttgacgatgg atttgatgat tccgtgttcc gtgagtgggt
      481 ggttacacca gagcgcccgc tcagggagtg gttttaa
//
LOCUS       DAZFRG010000655          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZFRG010000655 DAZFRG010000000
VERSION     DAZFRG010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.658"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..126)
                     /locus_tag="ACHKUC_005352"
     CDS             complement(<1..126)
                     /locus_tag="ACHKUC_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO6621362.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLE"
     gene            complement(99..218)
                     /locus_tag="ACHKUC_005353"
                     /pseudo
     CDS             complement(99..218)
                     /locus_tag="ACHKUC_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(237..515)
                     /locus_tag="ACHKUC_005354"
     CDS             complement(237..515)
                     /locus_tag="ACHKUC_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621363.1"
                     /translation="MLHALCAVAVQVLAGLFTGNWAYGAIAGCTFFIAREHTQAEYRW
                     IEMFGHGKRINMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
BASE COUNT          137 a          164 c          104 g          112 t
ORIGIN      
        1 ttcaaggtac tgtagtaatg ttttccgctt cagggaaaaa atagcatcca accgcagcac
       61 gttcttgctt aagacgtgct gcggcataat cccaatgatt actccctgac agggttcgta
      121 ggccactcaa tatcaggtgc agttgatgta tcaacacggt tcagcaacac cctgcatgcc
      181 ttccatgccg ccagcaatga tgtttcctcc ttcgttgcat atactgctca cctttttcac
      241 ccacgattaa ccaacagcca gaccagcaga cacgccacca ccggcacagc aaaatccatc
      301 aggcttgcca catcccatgc acgtggatca aaaccgcccc accacggcat gttaatccgc
      361 ttgccatgcc cgaacatttc aatccagcga tattctgcct gggtgtgttc acgcgcaatg
      421 aagaacgtac aaccggctat cgccccgtaa gcccagtttc cggtaaaaag accagccagt
      481 acctgcaccg ccacggcaca aagcgcatga agtatcg
//
LOCUS       DAZFRG010000656          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZFRG010000656 DAZFRG010000000
VERSION     DAZFRG010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.659"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>514
                     /locus_tag="ACHKUC_005355"
     CDS             <1..>514
                     /locus_tag="ACHKUC_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO6621364.1"
                     /translation="MISGGSTKNTEKIDFTITNKGDEAEALSLEVLDGNEVIYSERQT
                     GKYCDKGEHAWQWDGYSNQGILDTTKLKSKSLLVRLIALCGDMMIKVDYPLHNSPEEQ
                     KWVDVKVDRKQKTVDIIWRLAVSDGGIKGSNPKLSPVPYNDLVNLTKNGVEFYWSRNG
                     SRGGGIGENIV"
BASE COUNT          182 a           70 c          130 g          132 t
ORIGIN      
        1 aatgattagc ggtggtagta cgaagaatac agaaaaaata gattttacca tcacaaataa
       61 aggcgatgag gctgaagcgc tttctctgga agtgcttgat ggtaatgaag tgatttatag
      121 tgaacggcag accggtaaat attgtgataa gggagagcat gcctggcaat gggatggcta
      181 cagtaatcaa ggcatactgg atacaactaa gctaaaaagc aaatcactac ttgtaagact
      241 gattgcttta tgtggtgata tgatgataaa agttgattat ccacttcata attcaccaga
      301 ggaacaaaaa tgggtcgatg taaaggtaga cagaaaacaa aaaacagtag atataatatg
      361 gcgactggca gttagtgatg gagggataaa aggaagtaat cctaagttat ccccagttcc
      421 ttataacgac ttggttaacc tgaccaaaaa tggagttgaa ttttattggt ctcgaaatgg
      481 aagtcgcggc ggggggattg gtgaaaatat tgtt
//
LOCUS       DAZFRG010000657          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZFRG010000657 DAZFRG010000000
VERSION     DAZFRG010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.660"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..>513
                     /locus_tag="ACHKUC_005356"
     CDS             36..>513
                     /locus_tag="ACHKUC_005356"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6621365.1"
                     /translation="MRNEVWFREPSATWDDGITSFLVNYNFSGTSQSYDGNKTGNTYG
                     SFESQINYGAWRLKNFSNYVRSSYQKKFKSVSSYLERGFDFFQGGRLTIGQTTTDGTI
                     LDSIPLEGITISSDDGMLKPMLAQYSPTIRGIANSPARVIVKQNGNIIYEKNVPAGE"
BASE COUNT          181 a           77 c          113 g          142 t
ORIGIN      
        1 ataacgcaat ttatataaaa atccctcagg cgtcgatgag aaatgaagtt tggtttcggg
       61 aaccatcagc aacatgggat gatggaataa cttcttttct tgttaactat aacttttcag
      121 ggacatcaca aagttacgat gggaataaaa caggaaatac ttatggtagc tttgagagcc
      181 aaataaacta tggcgcatgg cggttaaaga attttagtaa ttatgtgaga agtagttatc
      241 aaaaaaaatt taaatctgtt agctcttatt tagaaagagg ttttgatttc tttcagggag
      301 gtcggttgac gataggtcaa acgacaacag acggtaccat actggatagc attcctcttg
      361 aggggataac aatatcctca gatgatggga tgttaaaacc aatgcttgcg cagtacagtc
      421 cgactattcg tggaatagca aattcaccag caagagtaat agtgaaacag aatggcaata
      481 taatatatga aaaaaatgtg cctgcaggtg aat
//
LOCUS       DAZFRG010000658          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZFRG010000658 DAZFRG010000000
VERSION     DAZFRG010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.661"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(268..502)
                     /locus_tag="ACHKUC_005357"
                     /pseudo
     CDS             complement(268..502)
                     /locus_tag="ACHKUC_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          135 a          127 c          124 g          116 t
ORIGIN      
        1 tggcgaattt tctctgcggg gcgtttgtgt ttctgacgga aatacgaagg aggaagctga
       61 gagctcatga cggatattac tgacagacat acacttcgcc gtatgtcctg gtcggaactc
      121 ttcacggcag cacaggaagc ggaatttcag cgtgattacg agcgtgccag aatagtgtgg
      181 tcattcgcct tacatgttgc aacaaccacg attaacaaaa atctggccat agctcatatt
      241 cgacgctgca atactttgtt gcataaatca aaaacagtac ccggaaacaa cacggggggc
      301 cggtcagtgt gcctgagacc ccaacatccc cggagatgac tgacattccg gactgacgca
      361 gttgctgcat atctgtggca atacgttgta tccagccaga caggttatcc cgcgtagtga
      421 caattaaccc gctttcctgc accagcttca gccgggccgt cggggatttt accagccgga
      481 aatatcagta aaacaacata tc
//
LOCUS       DAZFRG010000659          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZFRG010000659 DAZFRG010000000
VERSION     DAZFRG010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.662"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>500
                     /locus_tag="ACHKUC_005358"
     CDS             <1..>500
                     /locus_tag="ACHKUC_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6621366.1"
                     /translation="SEKRITNTAQVEKSARYLGTGSQWTVQGDRIKPGMDFWFYVRSV
                     NLVGKSAFVEASGQASNDAKGYLEFFRGLIDETLLGQALKERIDASALRTEVTQLEED
                     IRQRMDTDIAEVTRKIGKAENSLTQLVAKKNEDQTLAIAQVSQKVDRVSSEISQTVSQ
                     GQSENAR"
BASE COUNT          135 a          112 c          165 g           88 t
ORIGIN      
        1 tcggaaaaac gcatcacgaa cacggcacag gtggaaaaat ctgcccgtta tctggggacc
       61 ggcagtcagt ggactgtcca gggcgatcgg attaagccgg ggatggattt ctggttttat
      121 gtgcgcagtg tcaacctggt gggcaagtct gcatttgtgg aagccagcgg gcaggccagc
      181 aatgatgcaa aagggtatct ggaatttttc cgtggtctga tagatgagac gctgctgggg
      241 caggcactga aagagcgcat tgatgcttca gcgctgcgta cggaggtcac gcaactggaa
      301 gaagatatcc gtcagcggat ggacacggat atcgcagaag tgacccggaa aatcgggaag
      361 gcggaaaaca gcctcacgca gctggttgcg aaaaagaatg aggaccagac actggccatc
      421 gcgcaggtga gccagaaagt ggaccgggtg agcagtgaaa tctcacagac tgtcagccag
      481 gggcagtcag aaaacgcccg
//
LOCUS       DAZFRG010000660          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZFRG010000660 DAZFRG010000000
VERSION     DAZFRG010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.663"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>497
                     /gene="kfiC"
                     /locus_tag="ACHKUC_005359"
     CDS             <1..>497
                     /gene="kfiC"
                     /locus_tag="ACHKUC_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267195202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis
                     glycosyltransferase KfiC"
                     /protein_id="HFO6621367.1"
                     /translation="GNFITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNG
                     KIQFFYDDKATRMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRL
                     LQPLILGLWGDSGLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGL
                     LSRYG"
BASE COUNT          162 a           73 c          117 g          145 t
ORIGIN      
        1 cggtaatttc atcacctttc aagatgcaga tgatctttct catccggaga gaatacaaag
       61 acaagttgag gttcttcgca ataataaggc tgtaatctgt atggctaact ggatccgtgt
      121 tgcgtcaaat ggaaaaattc aattcttcta tgatgataaa gccacaagaa tgtctgttgt
      181 atcgtcaatg ataaaaaaag atatttttgc gacagttggt ggctatagac aatctttaat
      241 tggtgcagat acggagtttt atgaaacagt aataatgcgt tatgggcgag aaagtattgt
      301 aagattactg cagccattga tattggggtt atggggagac tccggactta ccaggaataa
      361 aggaacagaa gctctacctg atggatatat atcacaatct cgaagagaat atagtgatat
      421 cgcggcaaga caacgagtgt tagggaaaag tatcgtaagt gataaagatg tacgtggttt
      481 attatctcgc tatggtt
//
LOCUS       DAZFRG010000661          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZFRG010000661 DAZFRG010000000
VERSION     DAZFRG010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.664"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>496
                     /locus_tag="ACHKUC_005360"
     CDS             <1..>496
                     /locus_tag="ACHKUC_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621368.1"
                     /translation="KEVSLFSHGGPIHGPTTSDSVNTPSVPKYPQQMDIIGGWDSIDF
                     NWSNNAMFVMYGCRTSYASDDSGQGFASKLSLLDNFKDVNVWGQTESTYPSYFPDIRT
                     TSIMRSINIGWSFSPTYMVASSEGQGWDALFPDDKNPLKSLPMQCYNNGKLILTCDQS
                     SFNDH"
BASE COUNT          155 a           91 c           97 g          153 t
ORIGIN      
        1 aaagaagtca gtttattctc acatgggggg ccaattcatg gtcctacaac ctcagactct
       61 gtaaatacac cttccgtgcc taaataccca caacaaatgg acataatcgg aggatgggat
      121 tcaattgatt ttaactggtc aaataacgct atgtttgtta tgtatggttg tcgcactagt
      181 tatgcaagtg atgattcagg acaaggtttt gccagtaaat tatccctatt agataatttt
      241 aaggacgtta atgtctgggg acaaacagaa agtacttatc catcttattt cccagatatt
      301 aggacaactt cgataatgag gtcaataaat ataggttggt ctttttcacc aacatacatg
      361 gttgccagtt ctgaaggtca aggatgggat gccttatttc cggatgataa aaatccacta
      421 aagtctctcc ctatgcagtg ttataataat gggaaattga ttttaacctg cgatcaaagc
      481 agttttaatg atcacc
//
LOCUS       DAZFRG010000662          493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZFRG010000662 DAZFRG010000000
VERSION     DAZFRG010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.665"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>493)
                     /locus_tag="ACHKUC_005361"
     CDS             complement(<1..>493)
                     /locus_tag="ACHKUC_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6621369.1"
                     /translation="LQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAE
                     ILCGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHER
                     AEIIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYIWPWIL
                     PFSA"
BASE COUNT          138 a          118 c           97 g          140 t
ORIGIN      
        1 ccgctgaaaa cggcaatatc cagggccaga tatattcacc attcatattt ctgcatttct
       61 gtacaatttt ctcagaaagc gtctcatcaa ttctttcacg caacttctcc tgctgtctta
      121 aaatactttc gcatatttcg aaatcatctt cctgcatcag catttcggcg catttaatga
      181 tttctgcccg ttcatgcagc acagaggaat gattatagag ccagtttaca aacagcaatg
      241 cttccggata ttgcccccat aacgggtggt atgtggcata ataatcttcg gcagtatccg
      301 gaccgcctga aaagttatgc catacagcaa gtggaaatgg atgcagattt ccgcacagta
      361 tttctgcgat gtaagtattt gccgcaaagc cgcgacgaaa tgcagttgca gcttttacca
      421 gttccccatt aagaaaatgc agaaggccga gttcatacca gtaaggtgaa aattcatctg
      481 catgttcctg cag
//
LOCUS       DAZFRG010000663          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZFRG010000663 DAZFRG010000000
VERSION     DAZFRG010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.666"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          136 a           95 c           97 g          164 t
ORIGIN      
        1 gcgacattta cgttcatctt cttgcagaca ttctccagtc agcctgcagg ttatagctat
       61 tcctgactga ttcgtcattc tattcttatt gattataact ggcattacac cggtgccgga
      121 gtgtttttct gtaagtctgc gccggttcag taaattagaa aagaaacatt tgtcagcgcc
      181 agtgatataa ggcgtaacgg ctaacaatct tggcgttgat acattccgag caaataaccg
      241 aagtgttact tttagtgacg gggttatcca atacttttct agtcagtgtt tagtatttat
      301 gcaagcgatg taagtagtaa ccgcttagac tattgacatt catgatgcct gttaattcaa
      361 atttcaggta tgatctgtca tttattgttg tgatactgat tctgctaggt tggcataact
      421 gtcctgcaaa tcattgttca aaaaaagaaa gctcctacaa actgtatcct gtatctctac
      481 cagaagataa ga
//
LOCUS       DAZFRG010000664          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZFRG010000664 DAZFRG010000000
VERSION     DAZFRG010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.667"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..>491
                     /gene="evgA"
                     /locus_tag="ACHKUC_005362"
     CDS             67..>491
                     /gene="evgA"
                     /locus_tag="ACHKUC_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO6621370.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKHQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKL"
BASE COUNT          152 a           93 c          110 g          136 t
ORIGIN      
        1 gatgatacct tatgtctgta ttactacaag gagaagggaa atgcttcatt gcaaagggaa
       61 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
      121 ttattgatca aaaacgatat tgaaatctta gcggagttga ctgaaggggg aagtgccgtt
      181 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
      241 aacggtatcc aggtgttaga aacgctgagg aagcaccaat atagcggaat tattattatc
      301 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatgga
      361 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
      421 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
      481 caaaaactcg a
//
LOCUS       DAZFRG010000665          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZFRG010000665 DAZFRG010000000
VERSION     DAZFRG010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.668"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..491
                     /locus_tag="ACHKUC_005363"
                     /pseudo
     CDS             1..491
                     /locus_tag="ACHKUC_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          115 a          124 c          142 g          110 t
ORIGIN      
        1 ttctctggcg taagcaatac caggaaggaa gtcttactgc tgtcgccgcc ggagaacagg
       61 ttgttcctgc ctctgaactt gctgccgcca tgaagcagat taaagaactc cagcgcctgc
      121 tcggcaagaa aacgatggaa aatgaactcc tcaaagaagc cgttgaatat ggacgggcaa
      181 aaaagtggat agcgcacgcg cccttattgc ccggggatgg ggagtaagct tagtcagccg
      241 ttgtctccgg gtgtcgcgtg cgcagttgca cgtcattctc agacgaaccg atgactggat
      301 ggatggccgc cgcagtcgtc acactgatga tacggatgtg cttctccgta tacaccatgt
      361 tatcggagag ctgcccacgt atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      421 agaacttgat ggtatgcctg cgatcaatgc caaacgtgtt taccggatca tgcgccagaa
      481 tgcgctgttg c
//
LOCUS       DAZFRG010000666          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZFRG010000666 DAZFRG010000000
VERSION     DAZFRG010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.669"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>484
                     /locus_tag="ACHKUC_005364"
     CDS             <1..>484
                     /locus_tag="ACHKUC_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO6621371.1"
                     /translation="YRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEF
                     RCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDVMLGAVERRFGSELPASPVE
                     WLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPD
                     G"
BASE COUNT          124 a          113 c          155 g           92 t
ORIGIN      
        1 tttaccggat catgcgccag aatgcgctgt tgcttgagcg aaaacccgct gtaccgccat
       61 cgaaacgggc acataccggc agagtggctg tgaaagaaag taatcagcga tggtgctctg
      121 acgggtttga gttccgctgt gataacggag aaaaactgcg ggtcacgttc gcgctggact
      181 gctgtgaccg tgaggcactg cactgggcgg tcacaacggg tggcttcgac agtgaaacag
      241 tacaggacgt catgctggga gcagtggaac gccgctttgg cagcgagctt ccggcgtctc
      301 cagtggagtg gctgacggat aatggttcat gctaccgggc gaatgaaaca cgtcagttcg
      361 ccaggatgtt gggacttgaa ccgaagaaca cggcagtgcg gagtccggag agtaacggaa
      421 tagcagagag cttcgtgaaa acgataaagc gtgactacat aagtatcatg cccaaaccag
      481 acgg
//
LOCUS       DAZFRG010000667          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZFRG010000667 DAZFRG010000000
VERSION     DAZFRG010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.670"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..125
                     /locus_tag="ACHKUC_005365"
     CDS             <1..125
                     /locus_tag="ACHKUC_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO6621372.1"
                     /translation="KIYNKYRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            122..>479
                     /locus_tag="ACHKUC_005366"
     CDS             122..>479
                     /locus_tag="ACHKUC_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO6621373.1"
                     /translation="MKRKLQKIILYLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQDEIKSQNPLPEDSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNY"
BASE COUNT          161 a           83 c           77 g          158 t
ORIGIN      
        1 acaagattta caacaagtat agattttttc gcatatggtt ctttttacaa cggcataaag
       61 actttgcttt caaacctttc ttcaccaatt ttaacactat cgaaagaatc gaaaattatc
      121 tatgaaaaga aaactccaga aaattatatt atatttattt cttctatgct gcatctataa
      181 tttatggaca ttgcgtcctg tacagatttt gtatacatac tctgatgctg gcaattcagt
      241 ctttctggtc gttgatcatc tgccatggac tgatagcgat aaaataaatt ggtatctgaa
      301 gcatcaggat gaaattaaaa gtcaaaatcc cctaccagaa gatagttggc atacctggta
      361 tgttattgat atcggtaatg gcttcactga ctataaaaaa tatatcgaag ggccgtatga
      421 agatctctac tgtttcccga cgattaagag caatgataat tgcatcgtaa aaaactatt
//
LOCUS       DAZFRG010000668          477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZFRG010000668 DAZFRG010000000
VERSION     DAZFRG010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.671"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..>477
                     /gene="gadE"
                     /locus_tag="ACHKUC_005367"
     CDS             155..>477
                     /gene="gadE"
                     /locus_tag="ACHKUC_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6621374.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLP"
BASE COUNT          171 a           76 c           90 g          140 t
ORIGIN      
        1 taaatctgaa gtattgatta gtgttaatag acgtattagt tcacgaaggg caaagttctt
       61 ataggcgttt actatattga acaacgattc ggacaaggat gtaaataatg aaaaggatga
      121 catgttcgaa acgataacgg ctaaggagca agttatgatt tttctcatga cgaaagattc
      181 ttttctttta cagggctttt ggcagttgaa agataatcac gaaatgataa aaatcaattc
      241 cctgtcagag atcaaaaaag taggcaataa acccttcaag gtgatcattg acacctatca
      301 caaccatatc cttgatgaag aagcgattaa attcctggag aaattagatg ccgagagaat
      361 tattgttttg gcaccttatc acatcagtaa actaaaagct aaagcgccta tttattttgt
      421 cagccgtaaa gaaagtatca aaaatcttct tgagattact tatggtaaac acttgcc
//
LOCUS       DAZFRG010000669          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZFRG010000669 DAZFRG010000000
VERSION     DAZFRG010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.672"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..314)
                     /gene="mchB"
                     /locus_tag="ACHKUC_005368"
     CDS             complement(87..314)
                     /gene="mchB"
                     /locus_tag="ACHKUC_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO6621375.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(342..>476)
                     /gene="mchI"
                     /locus_tag="ACHKUC_005369"
     CDS             complement(342..>476)
                     /gene="mchI"
                     /locus_tag="ACHKUC_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO6621376.1"
                     /translation="SLRIVGEGNSYVDVFLSFIIFLGFIELIHGIRRILVWSDWKNGS
                     "
BASE COUNT          160 a          124 c           78 g          114 t
ORIGIN      
        1 aatggatctt ctatctgttt taaacaaaaa ggatatattt tacaggtact accgaaaaag
       61 tgcctgtaaa ataaataaaa atataattag ctaccgccac cagcagaaga actggcacta
      121 ccacttccca cggtcgagcc aattgctgtt gtcaaaccgg cagatacggc tccaactaca
      181 accccaagtg gaccaccagg tattccggcg agagctccaa caatagctgc agcacctgca
      241 gcattagctg aagtcgctgg cgcacctccc gccccggaaa tatatcttaa ctgtgattct
      301 gttatttctc gcatataaac tgcatataaa ctccatcata tttaacttcc gtttttccag
      361 tctgaccaga ccagaatcct tcgaatccca tgaatcagct caataaaacc aagaaatatt
      421 ataaagctta gaaaaacgtc aacataagaa ttcccttcgc caacaatccg aaggga
//
LOCUS       DAZFRG010000670          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZFRG010000670 DAZFRG010000000
VERSION     DAZFRG010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.673"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..401
                     /locus_tag="ACHKUC_005370"
     CDS             <1..401
                     /locus_tag="ACHKUC_005370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621377.1"
                     /translation="SGADTRYSSPVQSGPEQECDVNVTIFGRLNITGADMAECSWLVS
                     CEGSMAEYSALKKAFACRLNVVKNTPVAELGGIRLIWQIIIIINQKELPCLSNGRNRG
                     NIFSVPGNPAEIKNVMKFKERKTGLLWLNA"
BASE COUNT          138 a           89 c          116 g          132 t
ORIGIN      
        1 tttcaggggc agacacccga tattccagtc cagtccagtc cggtcctgaa caggaatgtg
       61 atgttaacgt cacgatcttt ggtcgtctta atatcactgg tgctgatatg gcagaatgca
      121 gttggctggt atcttgtgaa ggaagtatgg cggagtattc tgctctgaag aaagcattcg
      181 catgccggtt aaatgtcgtt aaaaataccc ccgtcgcgga gcttgggggg atcagactta
      241 tatggcagat cattattatt ataaatcaga aggagttgcc ctgtctttca aatggacgta
      301 atagaggtaa catattttct gtcccgggta atcctgcaga aataaaaaat gtgatgaaat
      361 tcaaggaacg gaaaacaggc ttgttatggc tgaatgcata acagaaacgc gggataaccg
      421 ttacgggaaa atatcccttt ttctgtgtat ccacagcaat atgtgactta aatct
//
LOCUS       DAZFRG010000671          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZFRG010000671 DAZFRG010000000
VERSION     DAZFRG010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.674"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..>472
                     /locus_tag="ACHKUC_005371"
     CDS             48..>472
                     /locus_tag="ACHKUC_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFO6621378.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAV"
BASE COUNT          156 a           71 c          106 g          139 t
ORIGIN      
        1 atagttaccg tgtcggtgtt ctgattaatt tttaaaaggt gattacaatg aaagtgaaaa
       61 actttatata tcttcccttc tgcttgttta ttggaacgtc ggtagccgga gctttaccgg
      121 aaattcctga accatttaaa tatggtgtag gagggatcga gaatggtaaa atatatatag
      181 gactaggaag tctgggaaat aactggtata tgatagatac aaaccagagt gaaaaaaaat
      241 ggactaagat agcgcaatgg ccgacagttc ccagagagca ggcaacagct actattattg
      301 atggtaagat ctatgtattt ggaggaattg gaaaagataa aagtggagtc ataaccttac
      361 aaaaagatgt ctattcctat gatattgcta aagataaatg ggaaaaattg atgacccgtc
      421 cccctgtatc tcttgccggt catgtttctt ttatccataa tggtcatgcg gt
//
LOCUS       DAZFRG010000672          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZFRG010000672 DAZFRG010000000
VERSION     DAZFRG010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.675"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..113)
                     /locus_tag="ACHKUC_005372"
     CDS             complement(27..113)
                     /locus_tag="ACHKUC_005372"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6621379.1"
                     /translation="MSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(194..>471)
                     /locus_tag="ACHKUC_005373"
     CDS             complement(194..>471)
                     /locus_tag="ACHKUC_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621380.1"
                     /translation="NIDEASGCFILRQRIDIVNAGRAKAFSRLTVLFCTPDRLSGRDV
                     IILNSDAIQRVCDEFMVANSELFALVQEYNRIASTCGMDELRITHLG"
BASE COUNT          149 a          135 c           77 g          110 t
ORIGIN      
        1 aatttctccc gctaactgat acccggctaa cagtctggcg ttttcttttt cagcaacggg
       61 aaagcaacaa ccaccacacc cgccaccagc acaccgtcag ccagcactga cattatccgg
      121 ctgctgcaat gccactcaca aaaacagtaa gcaatcactt tttaccgtaa caagtgataa
      181 tccagatatg tatctacccc agatgagtaa tccgaagttc atccatacca caggtactgg
      241 ctattctgtt gtactcctga acaagagcaa ataattctga attagcaacc atgaactcat
      301 cgcaaaccct ctgtatagca tcactattca gaataataac gtctcttccc gaaagacgat
      361 caggagtaca gaacaaaact gtcaaacggc tgaaggcctt tgctcgtcct gcattgacta
      421 tatcaatacg ctgcctaagg atgaaacacc ccgacgcctc atcaatattc a
//
LOCUS       DAZFRG010000673          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZFRG010000673 DAZFRG010000000
VERSION     DAZFRG010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.676"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(145..336)
                     /gene="yfgG"
                     /locus_tag="ACHKUC_005374"
     CDS             complement(145..336)
                     /gene="yfgG"
                     /locus_tag="ACHKUC_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO6621381.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            354..>468
                     /locus_tag="ACHKUC_005375"
     CDS             354..>468
                     /locus_tag="ACHKUC_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621382.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMKVN"
BASE COUNT          144 a           88 c          118 g          118 t
ORIGIN      
        1 cgcagccgaa caggatgaac ttcagtcaga acaaagacgt aaaatttaag atgaaaaaat
       61 atctgccgct ttgataatcc caaagagcaa aagcatccga cagcgaggtg ccgttatgtt
      121 ctgtgagagt ggagaaggta accgctaacg ttgtaccggt gattcgacgg agagtgtgga
      181 ttgctgagct tcttttttgc tctgatggtg ctgccaggca ccgacggacg agtagataaa
      241 acggccaaag aagaagataa agctgatgag cagtacgata cgggtcatgc gactgttaaa
      301 tcggtgtcgt tttcgcatac tggttgcctg actcacaaaa ggttccttga agtatgtccc
      361 acgccgtgga cggtacttac attaaggcac aacaggacaa aatggtcaat tcttcgttat
      421 gtaaaaaagc gtcagttgat acatatttta atgttatgaa agttaatt
//
LOCUS       DAZFRG010000674          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZFRG010000674 DAZFRG010000000
VERSION     DAZFRG010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.677"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..135)
                     /locus_tag="ACHKUC_005376"
                     /pseudo
     CDS             complement(43..135)
                     /locus_tag="ACHKUC_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(391..>468)
                     /locus_tag="ACHKUC_005377"
     CDS             complement(391..>468)
                     /locus_tag="ACHKUC_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO6621383.1"
                     /translation="HLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          138 a           95 c           91 g          144 t
ORIGIN      
        1 tattgtccga tcgttgaatc atttgggcgt tataacctaa atttttaccg tccaataatc
       61 aatatttcta ttgtgattgt taaccactgc gtgcattgaa gggtgaaaaa tatgtgcaat
      121 tgagaagcct ttcattgatg cctgccttgt catcatgaca aacaataata gatgcttatt
      181 ttcctcgcag ggttctttcg cactatcaaa aaatgctcat ttacccggca ttacagtcaa
      241 cgaagcaaca ttctatggcc tacgctgatg taaagcacag cgaaaatcct ccttgttgac
      301 ttcgtctgac cttatcgcgg cgaccagcaa ttattcgttt tgtgatcctc aacacaaaat
      361 gctattcgtt gcctatgagg ggagaagaat ttatggaaaa cgattgcatt cgcaaaatga
      421 cgtcctgtca gaagagaaaa agttgacgtt aatcaattgt tcgagatg
//
LOCUS       DAZFRG010000675          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZFRG010000675 DAZFRG010000000
VERSION     DAZFRG010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.678"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..338)
                     /locus_tag="ACHKUC_005378"
     CDS             complement(<1..338)
                     /locus_tag="ACHKUC_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HFO6621384.1"
                     /translation="MGNYHIGDGECRFRVVCSSPDVCEVGGYKVPFDSYQTLDSERQY
                     SSTVWARGCRALNVGSVIAGTQSNAGKGVISGTSQGTGDCVILTGSPTVTIEGKPVAY
                     HGSVVGINNH"
BASE COUNT          141 a          121 c           64 g          142 t
ORIGIN      
        1 ttgtggttat taatcccaac aactgaacca tgataagcaa caggtttccc ttctattgtt
       61 acagtcggac ttcccgttaa aataacacag tcacccgttc cttgagatgt accggatatg
      121 acacctttac cggcattact ttgagttcca gcaatcactg aaccaacatt taaagctctg
      181 catccacgcg cccataccgt tgaactgtac tgtctttcac tatctaaagt ctggtaactg
      241 tcaaaaggaa ccttgtaacc tcctacttca catacatctg gtgaagaaca cacgactcta
      301 aatctacatt caccatcccc gatatgatag ttacccataa tattttttcc ctatacattc
      361 aggaataata caactcagca ctccttcttt cattccaaaa aaatcaggta ttgttaatct
      421 ccaggtaagg tatatttcct gtcgacttaa atcgataacc attgtatc
//
LOCUS       DAZFRG010000676          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZFRG010000676 DAZFRG010000000
VERSION     DAZFRG010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.679"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>459
                     /locus_tag="ACHKUC_005379"
     CDS             <1..>459
                     /locus_tag="ACHKUC_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO6621385.1"
                     /translation="DTLLTRMNDLNRDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIG
                     QSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTRGIPGVALTILAFSAIF
                     IYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKV"
BASE COUNT          143 a           81 c           83 g          152 t
ORIGIN      
        1 agatacatta cttacgagaa tgaatgactt aaatagggat ttagttaatt attcgcatga
       61 taacaccaga acttcagtcg gtgcccgtct ggcaatgtat gaagttggtt taaaaacata
      121 ttctccaata ggacaatcac tggaaaaacg tgcggaaaaa atacatgaac tagaagagaa
      181 agagcctaga ttaagtggtg ctttaccctt tgtagattct catttgcata acgatctcat
      241 agatacgtta tcaacgcgtg gtattcctgg agtagcatta acaattttag ccttctcagc
      301 aatattcata tatgccttaa gaactgctaa agaaccttat attttaatct tgcttttttc
      361 actactggta gtaggcctaa gtgatgtaat actcttttct aaaccggttc cgactgctgt
      421 gtttgtcacc ataatattgc tttgtgctta ttttaaagt
//
LOCUS       DAZFRG010000677          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZFRG010000677 DAZFRG010000000
VERSION     DAZFRG010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.680"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..441)
                     /locus_tag="ACHKUC_005380"
     CDS             complement(<1..441)
                     /locus_tag="ACHKUC_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621386.1"
                     /translation="MITLLYCASSSAKSGSPPASTITFIAQIEHVELNKTALSHDMKL
                     ILADRFYFKTSNPCETNSISARPESMGITDKAYADKLRNLETPFLDDRYFFMTINQCD
                     GNGSPMLTDIKVCTTELCGAEYTKKIPTVWLDETLKPTAKRLAIY"
BASE COUNT          133 a           71 c           99 g          156 t
ORIGIN      
        1 ataaattgct aggcgtttag cagttggttt taatgtttca tcaagccata cagtaggtat
       61 tttctttgta tattcagcac cacataattc tgttgtacat acttttatat ctgtcagcat
      121 ggggctacca ttaccgtcac actgattgat ggtcataaag aaatagcgat catcaagaaa
      181 aggagtttct aaatttctta gtttgtcggc ataggctttg tcagtaatgc ccattgattc
      241 tggtcgagcg gaaattgaat ttgtttcaca aggattactg gtcttgaaat aaaaacgatc
      301 ggcaagaatg agtttcatat catgagataa tgctgtttta tttagctcaa catgctcgat
      361 ttgtgcgatg aaagttatag tgcttgcggg aggggatcca ctttttgctg atgaactggc
      421 gcaatacaat agagtgataa tagtaagtaa tggttttac
//
LOCUS       DAZFRG010000678          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZFRG010000678 DAZFRG010000000
VERSION     DAZFRG010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.681"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            318..398
                     /gene="shoB"
                     /locus_tag="ACHKUC_005381"
     CDS             318..398
                     /gene="shoB"
                     /locus_tag="ACHKUC_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO6621387.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT          132 a           93 c           95 g          134 t
ORIGIN      
        1 gattatggat caatttcgtt gacctgtaca agcaacttgc aatctctgac ataaaaaacg
       61 cgagccttta cgaagaaatc gacattgatg aaaagattaa cgtgaccgct ttttgataag
      121 tacattaaaa tcggtttcgt tattggcaat tttgctgtgc tttacaccat gccactgaat
      181 tcccccattg aaacgagtgg tgtcgtcaaa gctctggtgt ggattgcagc actcgattcc
      241 aataactcgc acgttcagtt ttggggagat gtaagggcta atctgaacgg ctgcattcct
      301 tgtttaagga aaaacgaatg actgattgcc gatacctgat taaacgggtc atcaaaatca
      361 tcattgctgt tttacagctg atccttctgt tcttataaca taaggaaacg tacttaaggt
      421 gcatccggtg aaccaatcgg acgcgccttt aata
//
LOCUS       DAZFRG010000679          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZFRG010000679 DAZFRG010000000
VERSION     DAZFRG010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.682"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>453
                     /locus_tag="ACHKUC_005382"
     CDS             <1..>453
                     /locus_tag="ACHKUC_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6621388.1"
                     /translation="YVDKKGSEITSTTDKKLGDQAVTIQQIQRVQSDTRNELNAMYML
                     KVQKTKNGIPYVAGIGAGIEDVDGQTLSNILLQADRIAMITPENGNTTPLFVAQGNQL
                     FMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTARNADISGAITANTGT"
BASE COUNT          117 a          113 c          134 g           89 t
ORIGIN      
        1 tacgtggata aaaaagggag tgaaattacc tcgaccacgg ataaaaagct gggtgaccag
       61 gccgtgacca tacagcaaat ccagcgggtt cagtcagaca cgcgcaatga gctgaatgcc
      121 atgtatatgc tgaaggtgca gaaaacaaaa aacggtattc cctatgtggc cgggattggc
      181 gcggggattg aggatgttga tggtcagacc ctgagtaaca ttctgctgca ggccgatcgc
      241 attgcgatga ttaccccgga gaacggcaac accacgccgc tgtttgtggc gcaggggaat
      301 cagctgttca tgaacgacgt gttcctgaag cgactgtttg cggtgagcat cacgtcatcc
      361 ggcaatcctc cgacgttttc cctgacgccg gatggcaggc tgacagcccg caatgcggat
      421 atcagtggag ccatcacggc gaataccggc acg
//
LOCUS       DAZFRG010000680          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZFRG010000680 DAZFRG010000000
VERSION     DAZFRG010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.683"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>445
                     /locus_tag="ACHKUC_005383"
     CDS             <1..>445
                     /locus_tag="ACHKUC_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621389.1"
                     /translation="PFWNYDYNAAQRNREIVDSYQQTNEARLNSQQAQFEASMANDRV
                     SRIQVQLNNTINSHKKAIADYEQRLEEQKAISFKLIMKVNIFERTLNRLQEQWPEKKE
                     SILDEIQHQKDYCSVEEYKEKWWKWVNDGGLTPEANCLKFPYPERE"
BASE COUNT          167 a           74 c           96 g          108 t
ORIGIN      
        1 tccattctgg aattatgatt ataatgcagc acaacgcaac agagagatag tagattcata
       61 tcaacagact aatgaggcca gactgaactc tcagcaagct cagtttgaag catctatggc
      121 taatgacagg gttagtagaa tacaggtgca gttgaataat acaatcaaca gtcataaaaa
      181 ggctattgct gattatgagc agcgtttgga agaacaaaaa gcaatttctt ttaaattgat
      241 catgaaggta aatatatttg agagaacgtt aaatcgcctt caagagcaat ggcctgaaaa
      301 aaaagaaagc atcttagacg aaattcagca ccagaaagat tactgttcgg ttgaagaata
      361 caaagagaaa tggtggaaat gggtcaatga tggtggcctt acaccagaag ctaattgctt
      421 aaaattccct tacccagagc gcgag
//
LOCUS       DAZFRG010000681          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZFRG010000681 DAZFRG010000000
VERSION     DAZFRG010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.684"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          180 a          101 c           38 g          125 t
ORIGIN      
        1 ataatcatat caaaataaaa attcacaaca gtgcaacatt aaaaaataca accaacaaac
       61 aatcctatat acaaggcaca tctccagaat ataaaagcac agacaaacaa cctaaaaaaa
      121 aaccatacat ataaattatt ttataggtaa aatagattat atattctcat agctaccaca
      181 aactatacta gcctgaacta tatttattct gctgcaatca atgcatacat aacacaaata
      241 tcactcaggt acactactca aaccacgctg ggatttttcc tcaagttata atcatcaccc
      301 cgaaaatcat tcggcattta ctcattaaat agtcacccca taggcctgta catgtttact
      361 cagaaatata catccttttc tctgtcataa accctctgat taatcataga taaatacttg
      421 tgacaccaat ctttttcctt aaag
//
LOCUS       DAZFRG010000682          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZFRG010000682 DAZFRG010000000
VERSION     DAZFRG010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.685"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>444)
                     /locus_tag="ACHKUC_005384"
     CDS             complement(<1..>444)
                     /locus_tag="ACHKUC_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6621390.1"
                     /translation="KTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIA
                     TIIAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYP
                     FDEFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLT"
BASE COUNT          135 a           98 c           79 g          132 t
ORIGIN      
        1 cgtaagcccc atatataatt tttctcgacc gcaggccagg ttcagacgaa ggtagccctc
       61 cccctccgtt ccatagacat gccccgccat aatgcccact ttgccttcat gaattaatcg
      121 actgtagaat tcatcaaaag gataaccaat agcagaacaa tctatccagg aaaaatagca
      181 accatcagct ggccttacga tcaattctgg aatattttca ttcagatact gctgggtgaa
      241 ttgaatgtta gaggctatat gtgcctttaa tgactttaac catccctcac attcgttgta
      301 ggcaataata gttgcaatta tattcagtac tgatggggag tttacaaaat cgcgatacct
      361 cgatatcgtt ctgaacttat gattaaaatt atcgtcagtt gaaatcatat acgatcctgt
      421 tagtgcagga acattaaatg tttt
//
LOCUS       DAZFRG010000683          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZFRG010000683 DAZFRG010000000
VERSION     DAZFRG010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.686"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>444
                     /locus_tag="ACHKUC_005385"
     CDS             <1..>444
                     /locus_tag="ACHKUC_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621391.1"
                     /translation="SGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEASGQPSNDG
                     EGYLEIFRGLIDETLLGQALKERIDASALRTEVTQLEEDIRQRMDTDIAEVTRKIGEA
                     ENSLTQLVAKKNEDQTLAIAQMSQKVDRVSSEISQTVSQGQSENAR"
BASE COUNT          117 a          100 c          151 g           76 t
ORIGIN      
        1 gtccggcagt cagtggactg tccaggggag ccggattaag ccggggacgg atttctggtt
       61 ttacgtgcga agcgtcaacc tggtgggaaa atctgctttt gtggaagcca gcgggcagcc
      121 cagcaatgat ggtgaagggt atctggaaat tttccggggg ctgatagatg agacgcttct
      181 gggtcaggca ctgaaagagc gcattgatgc ttcagcgctg cgtacggagg tcacgcaact
      241 ggaagaagac atccgtcagc ggatggacac ggatatcgca gaagtgaccc ggaaaatcgg
      301 ggaggcggaa aacagcctca cgcagctggt tgcgaaaaag aatgaggacc agacactggc
      361 catcgcgcag atgagccaga aagtggaccg ggtgagcagt gaaatctcac agactgtcag
      421 ccaggggcag tcagaaaacg cccg
//
LOCUS       DAZFRG010000684          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZFRG010000684 DAZFRG010000000
VERSION     DAZFRG010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.687"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..327
                     /locus_tag="ACHKUC_005386"
                     /pseudo
     CDS             1..327
                     /locus_tag="ACHKUC_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a          102 c           66 g           98 t
ORIGIN      
        1 tataactgca caacaaatac cgataatgtt aaaacgccag acacaccgaa gtggccttgt
       61 gataaccggg aagaacaaca ccccattaat tccccattct ctggagaatg gatacacctc
      121 tgaacaattt gatttagcgc aacaacgcca acaaattaat gcgtgccata cgaacaccac
      181 atatacaaat gcagactact caaagttgag gctcaacttg taagtcttat taataacatt
      241 gaaacgataa gctcgacacc acttccgcaa aagacacaat caatacttaa tcagataaat
      301 aacattagac acgaaaagat taaaaacctc tgaatataga ttatggaaca atggatactc
      361 atgccacgcc caaaaattaa ctgagctcaa ctaaacagaa gagatataaa catcagacag
      421 gtttacgtta ctatcaggca tatc
//
LOCUS       DAZFRG010000685          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZFRG010000685 DAZFRG010000000
VERSION     DAZFRG010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.688"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHKUC_005387"
     CDS             complement(<1..260)
                     /locus_tag="ACHKUC_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6621392.1"
                     /translation="MTQTESAILAHARRCVPAESCGLVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQI"
     gene            complement(266..>438)
                     /locus_tag="ACHKUC_005388"
     CDS             complement(266..>438)
                     /locus_tag="ACHKUC_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6621393.1"
                     /translation="YRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVAN
                     FGGFLSINKLSQ"
BASE COUNT           93 a          151 c          101 g           93 t
ORIGIN      
        1 tttatctgca gccgccggtc agcctcgctc agccagggca gcccaccggg atgactgtgg
       61 accagtgcca caatctcccc ctgcatctct gcccgcagcc agtcttccgg tgcaatacga
      121 aaatacgcct ccggctctgc agagatattc acacaaggga tataccgctc cccctccggc
      181 gttctcacca ctaagccgca cgactccgca ggcacacacc gccgggcatg cgccagaatc
      241 gctgattctg tctgtgtcat tggatttact gcgaaagttt gttaatggaa aggaaaccgc
      301 caaaattagc caccatgccg cgcatctcac acccgcgcat gcacttgctg catctgtcct
      361 tacggatatc cgtggtgggg ttgtcgaact catccgccac cgcaggaccg ttatacccgc
      421 attcatctcc ccggtaat
//
LOCUS       DAZFRG010000686          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZFRG010000686 DAZFRG010000000
VERSION     DAZFRG010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.689"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /locus_tag="ACHKUC_005389"
     CDS             <1..390
                     /locus_tag="ACHKUC_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621394.1"
                     /translation="GLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGVLLS
                     ELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKSHAE
                     LENWAVARKREKRELELMIDEYLLYAC"
BASE COUNT          104 a          117 c          108 g          105 t
ORIGIN      
        1 ggactctatc aggtcgtcaa cccgggaaca ccaccgttca ttcctgccat tcaggaaggg
       61 ttaatgacca gcattcagac ccatgaaatc atgctgctta cacattttaa tctgggcggc
      121 gttttactca gtgagctaca tcgtctgggt gaaaaccggc tggcaaacag gctgaacagc
      181 ctgctccggc gtttcgacga tcgtgatctc taccacacgc tgatatggct gtgctggtat
      241 gatctgatgt gtgcccactc catgcagccc tggactgaag agttaaagca taaatcacat
      301 gcagagcttg aaaactgggc tgtggcgcgc aaacgggaaa agcgcgagct ggaactgatg
      361 attgacgagt acctgttata cgcctgttga ttcataacgc tcccgggcct ccgcacactt
      421 acttttcctc cgtc
//
LOCUS       DAZFRG010000687          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZFRG010000687 DAZFRG010000000
VERSION     DAZFRG010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.690"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHKUC_005390"
     CDS             <1..180
                     /locus_tag="ACHKUC_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621395.1"
                     /translation="DGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDVLFSEGIADRG
                     LKNTGGVLIQKRPQL"
     gene            223..>426
                     /locus_tag="ACHKUC_005391"
     CDS             223..>426
                     /locus_tag="ACHKUC_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621396.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNDRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQI"
BASE COUNT          119 a           67 c           88 g          152 t
ORIGIN      
        1 gatggtcgtt attggttaga ttttcgtaaa tccaaagagg gtataaggtg tatctcatgc
       61 agtattctgt atcacgataa aagtaaaggg cgtatcgatg tcttgtttag tgaaggtata
      121 gctgacagag gtttaaaaaa tacgggtgga gtgttaatcc aaaaacgccc gcaattataa
      181 taattcctgg taatatttgt tttcgtaaaa aggatttact ttatgcaacg tcaatataaa
      241 aaatacattc ttcttttttg gggtgttatg gatgttatcg cgatagccac atatctgttc
      301 tactcgcttg gtaatgatcg tatccctttt tattccgata tcgttcattc actttcttta
      361 cttaaggata tcggtgttga aggcggtttt tacgcttatg cagtcgccac tgttacgcta
      421 caaata
//
LOCUS       DAZFRG010000688          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZFRG010000688 DAZFRG010000000
VERSION     DAZFRG010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.691"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..349
                     /locus_tag="ACHKUC_005392"
     CDS             14..349
                     /locus_tag="ACHKUC_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6621397.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKVKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          152 a           85 c           74 g          114 t
ORIGIN      
        1 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
       61 atctttttca gtcatatgga gtaaagtaaa attaataatc tggtatcaat cggatgcttt
      121 cttacctcct gaaagtatat ttacactgac tcacacaggt atcatgctca ataacaaagt
      181 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
      241 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
      301 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
      361 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
      421 tgcca
//
LOCUS       DAZFRG010000689          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZFRG010000689 DAZFRG010000000
VERSION     DAZFRG010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.692"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>421
                     /locus_tag="ACHKUC_005393"
     CDS             <1..>421
                     /locus_tag="ACHKUC_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6621398.1"
                     /translation="GKATGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSTGQ
                     MVGGTAESTTINNNGRQVIWSSGVSRDTLIYTGGDQTVHGEAHNTRLEGGNQYVHKYG
                     LALNTVINEGGWQVVKAGGTAGNTTINQNGELKVHAGG"
BASE COUNT          118 a          100 c          128 g           75 t
ORIGIN      
        1 agggaaagct accggtacaa ttattaacca ggatggttac cagaccataa aacatggcgg
       61 actggcaacc ggaaccatcg tcaacaccgg tgcagaaggt ggtccggagt ctgaaaatgt
      121 gtccactggt cagatggtcg gggggactgc tgaatccacc accatcaaca acaatggccg
      181 acaggttatc tggtcttcgg gggtatcccg ggacactctc atttacacag gcggtgacca
      241 gacggtacac ggagaggcac ataatacccg gctggaggga ggaaaccagt atgtgcacaa
      301 atacggactt gcgctgaaca cggtaattaa cgaggggggc tggcaggttg ttaaggcagg
      361 tggtactgcc ggtaacacca ccataaatca gaacggcgag ctgaaggttc atgccggcgg
      421 a
//
LOCUS       DAZFRG010000690          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZFRG010000690 DAZFRG010000000
VERSION     DAZFRG010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.693"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>421
                     /locus_tag="ACHKUC_005394"
     CDS             <1..>421
                     /locus_tag="ACHKUC_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6621399.1"
                     /translation="GKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQ
                     MVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGG
                     TATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGG"
BASE COUNT          121 a          101 c          132 g           67 t
ORIGIN      
        1 agggaaagct gccggtacaa ttattaacca ggatggttac cagaccataa aacatggcgg
       61 actggcaacc ggaaccatcg tcaacaccgg tgcagaaggt ggtccggagt ctgaaaatgt
      121 gtccagcggt cagatggtcg gagggacggc tgaatccacc accatcaata aaaatggccg
      181 gcaggttatc tggtcttcgg ggatggcacg ggacaccctc atttacgccg gtggtgacca
      241 gacggtacac ggagaggcac ataacacccg actggagggg ggtaaccagt atgtacacaa
      301 cggtggcacg gcaacagaga cgctgataaa ccgtgatggc tggcaggtga ttaaggaagg
      361 aggaactgcc gcgcatacca ccatcaacca gaaaggaaag ctgcaggtga atgccggcgg
      421 t
//
LOCUS       DAZFRG010000691          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZFRG010000691 DAZFRG010000000
VERSION     DAZFRG010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.694"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..>418
                     /locus_tag="ACHKUC_005395"
     CDS             4..>418
                     /locus_tag="ACHKUC_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6621400.1"
                     /translation="MIENKIQTEVKKSRRGLPRHVKNPFLNDTNIHTKTGIRRITTGK
                     DRLAVVNENTGEQVGHGGFFQSMEVDKTQFVKLYVDGVSAIEGLSSSGKKVFKILYLA
                     IRDNKDTDTILMSFDIVDQEIVKISRTTYFKGMKEL"
BASE COUNT          164 a           63 c           86 g          105 t
ORIGIN      
        1 accatgatag aaaataagat acaaacggaa gtaaagaaat caagaagagg tctacctcgc
       61 catgtaaaaa acccattcct taatgataca aatatacata caaaaacagg tatcagacgt
      121 atcacaacag gaaaagaccg cttagccgta gtaaatgaaa atacaggaga acaggttggg
      181 catggtggtt tctttcagtc aatggaagta gacaagacac agttcgtgaa gctttatgtg
      241 gatggcgtat cagctattga gggtttatcc tcatccggta agaaagtatt taagatactg
      301 tatcttgcta ttagagacaa taaagataca gatacgatat taatgtcatt cgatattgtt
      361 gatcaagaga ttgtaaaaat atcaagaacg acatatttca aagggatgaa agaacttg
//
LOCUS       DAZFRG010000692          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZFRG010000692 DAZFRG010000000
VERSION     DAZFRG010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.695"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>411
                     /locus_tag="ACHKUC_005396"
     CDS             <1..>411
                     /locus_tag="ACHKUC_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6621401.1"
                     /translation="LVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETG
                     LGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVQTTSKGDRNPASVRLLIQLQRNGRW
                     VTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMV"
BASE COUNT           90 a          121 c          128 g           72 t
ORIGIN      
        1 ctggtgaaca aaaccccact gacggacacg gacggcaatc ccgtgataca cggtgtgacg
       61 gcggtctggc gtgccgggga gcaggagcag acaccaccgg aaggctttga gtcctccgga
      121 gctgaaaccg gactgggtgt ggaagtgacg aaggcaaagc cggtgacgcg caccattacg
      181 tccgcgaaca ttgaccgcct gcgggttacc ttcggggtgc agtcactggt gcagaccacg
      241 tcaaagggcg accgtaaccc ggcatccgtc cgcctgctga ttcagttaca gcgtaatggc
      301 cgctgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcgca gttcctggcc
      361 tcggtgattc tggataatct gccgccccgg ccctttaaca tccggatggt c
//
LOCUS       DAZFRG010000693          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZFRG010000693 DAZFRG010000000
VERSION     DAZFRG010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.696"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          129 a           90 c           67 g          124 t
ORIGIN      
        1 tgtaaatgtg agtccttgtt ccactctcgt acagcatcgc tggtcatacg cgaacacgta
       61 caaacagcaa tggtgaggcg gcatcaagag cagggatccg tcatttatgc aagcatttta
      121 ccccaaaaac cttttattta taagggtcat tgcgaattat ctgatgcaaa gttatgttat
      181 gttaggcaaa gtaatcttct attattaata agcacaacaa aacctttttg aatattaaat
      241 aataattaat tagcatcatc cccattcatt aattccgctt aacaatagtt tcacgccttg
      301 cccccattgt gacacctcgg aaatcatcac gctgttaaac tctccgtaat gcggaaatat
      361 catcatcgcg attcagtagt gaattcttaa acgaggcatt ttcacacaat
//
LOCUS       DAZFRG010000694          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZFRG010000694 DAZFRG010000000
VERSION     DAZFRG010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.697"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..>409
                     /locus_tag="ACHKUC_005397"
     CDS             83..>409
                     /locus_tag="ACHKUC_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO6621402.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPGGGEYWRLKYRV
                     AGKEKLLALGVYPEVTLADAPAKLEEAKRGISGGIDLMEVKREEKIARETQLNNTFKD
                     IALEWHS"
BASE COUNT          121 a           80 c          108 g          100 t
ORIGIN      
        1 tcttttgacg tctatttaag taccccaaac tctacaagca attgagggga tttgctgttc
       61 agtttagtgg agataccccc aagtgaaact caatgcccgt caaatagaca ctgccaagcc
      121 aaaagagaag gcttacaagt tggctgatgg tggtggtctg tacctcctgg taaaacctgg
      181 tggaggagaa tattggcgtc tcaagtatcg tgtagctggt aaagagaagc tgttagcact
      241 aggtgtgtat cctgaggtca ccttagctga tgctcctgca aaacttgaag aagctaaaag
      301 aggtatctct gggggaatcg atctgatgga agtgaagcga gaggaaaaga ttgcccggga
      361 aacgcagtta aacaacacct tcaaagatat tgcccttgag tggcacagt
//
LOCUS       DAZFRG010000695          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZFRG010000695 DAZFRG010000000
VERSION     DAZFRG010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.698"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          116 a           92 c           64 g          133 t
ORIGIN      
        1 ctccgtcagg agtagtatgg tttttctaga gcttcgcctc tcctaatccg catattaaag
       61 tttcaaaaca atctcatgat tttcaaattt tatacacgta ataggacaag acgcttctca
      121 tgctatgact tagaattgca atacgtttcc atattgcgac acccttctgc actcccatag
      181 acaacatcat agctataaaa acattttctc gttttgctaa agaaaattac actgctgttt
      241 ttataagcct tagatgtgcg cttcgagttg atgttacgca gactccccgc tttggcatcg
      301 acaataacct tgagaatgaa tcaaacactt tcgctcctac cttctcagac ccgtacaatc
      361 aggtttaacg ttattgcttt tgagaactag attagttttt ggaaa
//
LOCUS       DAZFRG010000696          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZFRG010000696 DAZFRG010000000
VERSION     DAZFRG010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.699"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>404
                     /locus_tag="ACHKUC_005398"
     CDS             <1..>404
                     /locus_tag="ACHKUC_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO6621403.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTG"
BASE COUNT           98 a           96 c          110 g          100 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctgg
//
LOCUS       DAZFRG010000697          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZFRG010000697 DAZFRG010000000
VERSION     DAZFRG010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.700"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..399
                     /locus_tag="ACHKUC_005399"
                     /pseudo
     CDS             188..399
                     /locus_tag="ACHKUC_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          115 a           87 c           99 g           98 t
ORIGIN      
        1 tgatacaagt ttttagagta gtcattagtg aacagataat tgttaactta gaaccagtaa
       61 aatgcaaacg acgcaccacg ctgggaaaat cgctgctatt cagcagagtt ttgtaccgag
      121 aattgtggcc agccacttat tgctctcgcg taagcaggta tccccatcgt ttaccagtcc
      181 agcacatctt gtggtcagac agaagaagag caaactgtta ctggactcac tgcatgcatg
      241 gtggagaaaa atgccacgct atccaaaaat ccagactggg tgaatggttc agctgtgtac
      301 tgaatcagtg gggcgcactc tgttattaca gtgttgacgg tcaggcggag gcagacataa
      361 tgccgcggaa aaagcccttt gtgccatctg tctcggaaa
//
LOCUS       DAZFRG010000698          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZFRG010000698 DAZFRG010000000
VERSION     DAZFRG010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.701"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>396
                     /locus_tag="ACHKUC_005400"
     rRNA            <1..>396
                     /locus_tag="ACHKUC_005400"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           96 a          103 c          118 g           79 t
ORIGIN      
        1 gtgccttcgg gaaccgtgag acaggtgctg catggctgtc gtcagctcgt gttgtgaaat
       61 gttgggttaa gtcccgcaac gagcgcaacc cttatccttt gttgccagcg gtccggccgg
      121 gaactcaaag gagactgcca gtgataaact ggaggaaggt ggggatgacg tcaagtcatc
      181 atggccctta cgaccagggc tacacacgtg ctacaatggc gcatacaaag agaagcgacc
      241 tcgcgagagc aagcggacct cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg
      301 actccatgaa gtcggaatcg ctagtaatcg tggatcagaa tgccacggtg aatacgttcc
      361 cgggccttgt acacaccgcc cgtcacacca tgggag
//
LOCUS       DAZFRG010000699          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZFRG010000699 DAZFRG010000000
VERSION     DAZFRG010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.702"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..110
                     /locus_tag="ACHKUC_005401"
     CDS             <1..110
                     /locus_tag="ACHKUC_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6621404.1"
                     /translation="VDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
     gene            149..214
                     /locus_tag="ACHKUC_005402"
                     /pseudo
     CDS             149..214
                     /locus_tag="ACHKUC_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           97 a          102 c           97 g           99 t
ORIGIN      
        1 acgtggatcc agaaagctac cttcgccatg tgcctggcgt catagcagac tggccggtca
       61 accgggtcag cgaactgctt ccgtggcgca tagcactgcc agctgaataa cacatccccg
      121 tcaatacggc cctcgctgta cgcttactgt tatgccggga gcggcaacgc tcgaccagca
      181 tggggaacaa attcgccagg agttctcatc ataagtgtaa gatgtgctgg taaatagctc
      241 ccacatttga acattgtggg agccgctatt tagtcattct gctgacagaa aacagcaatc
      301 tcctggtcca gctctgcaat gtgttctttt acagagttca ggagctgaag aatttcaggc
      361 ttactgtcat ctgaaacagg tgttatttct aactg
//
LOCUS       DAZFRG010000700          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZFRG010000700 DAZFRG010000000
VERSION     DAZFRG010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.703"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..257
                     /locus_tag="ACHKUC_005403"
     CDS             9..257
                     /locus_tag="ACHKUC_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6621405.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          126 a           83 c           83 g          102 t
ORIGIN      
        1 ggacttttat gcgtattgaa atctgcatag ccaaagaaaa aatgactaaa atgccaaccg
       61 gtgctgtgga tgcgttaaag gaagaattaa cccgacgcat cagtaaacgt tatgacgatg
      121 tagaggtgat cgtaaaagcc accagcaacg atggcctttc tgttacgcgc accgccgata
      181 aagattcagc taaaactttt gttcaggaaa ctctgaaaga tacctgggag tctgctgacg
      241 agtggtttgt tcactaatta gcacgtaaaa tcagtaacag ctggaaatca ttcaatactc
      301 gcactatcga aagttaacca gccagccgca gtatcctgtc atgacaagtt actgcggctt
      361 tttacttttt atatttaacg gatcaacatc caga
//
LOCUS       DAZFRG010000701          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZFRG010000701 DAZFRG010000000
VERSION     DAZFRG010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.704"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(229..>391)
                     /locus_tag="ACHKUC_005404"
     CDS             complement(229..>391)
                     /locus_tag="ACHKUC_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621406.1"
                     /translation="FMLITLLVILIAKPSTGLGEVLVTINLLNALVWLAINLVNRLRE
                     RLVSHRDQH"
BASE COUNT          119 a           87 c           74 g          111 t
ORIGIN      
        1 attttcatga cattgatcac tttggggtaa ctattttgtt taacaaataa ctcttcgaca
       61 tcaaaattat caacagttgc catggaaatt atttcactga tatataaaac aactgtgata
      121 acaccagggt tgacaactat ttgtgcaata attcctttcc ttcaatgatc caggggttca
      181 ctgccagcaa cccattcagc cgcatactgg cagtgagaaa ctgaaagatt agtgctgatc
      241 cctgtggctg acgagtcttt cccttaatcg attaaccaga ttgatcgcca gccaaaccag
      301 ggcattaagc aaatttatcg tcacaagcac ttctcccagc ccggtgctgg gttttgcaat
      361 aaggatgacc aggagagtga tcagcataaa t
//
LOCUS       DAZFRG010000702          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZFRG010000702 DAZFRG010000000
VERSION     DAZFRG010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.705"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..>390
                     /locus_tag="ACHKUC_005405"
     CDS             33..>390
                     /locus_tag="ACHKUC_005405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFO6621407.1"
                     /translation="MIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVAS
                     PEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDW
                     KKVGSLPEGLAYGVSLP"
BASE COUNT          105 a           73 c          107 g          105 t
ORIGIN      
        1 cgactccatt tccgggaact gctggttcat cggtgatttt tgcagaacag caaatttata
       61 tactgggagg ggagagaaaa ccggggcttc gttctgttag atcctggacg ggggagcttt
      121 cgcatgatcg aattaaatgg tctgaacttc caccagtagc ttcacccgag ggggtgtcag
      181 gagcatatgc ctctgttatt gatggaaata tctttctggc cggtggtgca tatttcccgg
      241 gggcggcaga aaagtatagc aatggtgaat actggtctca taaaggactg gataaagcct
      301 attcaaaaga gatatatcag ctaataaaaa atgactggaa aaaagttggt tctttgccgg
      361 aaggacttgc ttatggtgta agcctgccct
//
LOCUS       DAZFRG010000703          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZFRG010000703 DAZFRG010000000
VERSION     DAZFRG010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.706"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..330
                     /locus_tag="ACHKUC_005406"
     CDS             <1..330
                     /locus_tag="ACHKUC_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6621408.1"
                     /translation="ANGFVSKKEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQ
                     KLDSLSKQEISVMRYILDGKDNNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTF
                     AQRNKIG"
BASE COUNT          127 a           77 c           72 g          114 t
ORIGIN      
        1 gctaatggtt tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct
       61 gcaaaaaatg gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg
      121 tccgaccagc aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt
      181 cttgatggca aggataataa tgacattgct gaaaaaatgt tcatcagcaa caaaactgtc
      241 agcacttata aaagtcgctt gatggaaaaa ttagaatgca aatcactgat ggatctttac
      301 acattcgcac aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct
      361 tctctgttgt ggtctttggt cgaccataag
//
LOCUS       DAZFRG010000704          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZFRG010000704 DAZFRG010000000
VERSION     DAZFRG010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.707"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>386)
                     /locus_tag="ACHKUC_005407"
     CDS             complement(<1..>386)
                     /locus_tag="ACHKUC_005407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621409.1"
                     /translation="RIVIIKLIGTIFSVVIGVILIVSILSGDYFCRVGHGTCKAAKSA
                     RHLIESKEKNRSSRVKVFSTHINLSHPAASWLATQTCATNIFNLFQNNNAIYLFENDN
                     KIELRMGHDSFFSIETIDKINKENIK"
BASE COUNT          117 a           71 c           66 g          132 t
ORIGIN      
        1 ctttgatgtt ttctttgtta attttatcaa tcgtttcaat agagaaaaaa gaatcatgcc
       61 ccattcgcag ttcaatttta ttatcgtttt cgaataaata aatggcatta ttgttttgaa
      121 acagattgaa tatattggta gcgcaagttt gagttgctag ccaggatgcc gctggatgac
      181 tcaaattaat atgcgtgcta aagactttga cgcgactact tctgtttttc tctttacttt
      241 caattaaatg cctagcgctt ttggctgctt tacaagttcc atgccccaca cggcaaaaat
      301 aatctcctga aaggatactt acaatcagta ttacaccaat gacgacagaa aaaatagttc
      361 cgatgagttt gatgatgaca atcctt
//
LOCUS       DAZFRG010000705          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZFRG010000705 DAZFRG010000000
VERSION     DAZFRG010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.708"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..312)
                     /locus_tag="ACHKUC_005408"
     CDS             complement(<1..312)
                     /locus_tag="ACHKUC_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621410.1"
                     /translation="MEVIVKETDSIQAMSDSAVLSYVYEQSGFSIDLNKCFVRLFNNA
                     SGINAVYDYNQIREINYTLPNTDFGDAEICILTSGSNNALWKFTVKSEGSERVCERWI
                     NI"
BASE COUNT          119 a           85 c           65 g          117 t
ORIGIN      
        1 tatgttaatc catctctcac aaactctttc gctgccttcc gacttaacag taaatttcca
       61 taaagcattg ttgcttccag atgtgagaat acagatctct gcatcaccga aatcagtgtt
      121 tggtaatgtg taattgattt cccggatttg gttgtagtca tatacggcat taatgccaga
      181 tgcattattg aatagccgca caaaacattt attcagatca atagagaatc cagactgttc
      241 atagacatat gataaaacag cactgtcaga cattgcttga atgctgtcgg tttccttgac
      301 gatgacttcc ataaccacct caccagatcc tactgaacga cctacgcgaa gcatatcatt
      361 ttgtgaacag atctcaataa aatgat
//
LOCUS       DAZFRG010000706          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZFRG010000706 DAZFRG010000000
VERSION     DAZFRG010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.709"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..217)
                     /locus_tag="ACHKUC_005409"
     CDS             complement(<1..217)
                     /locus_tag="ACHKUC_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6621411.1"
                     /translation="MRKLCAAILSAAICLSVSGAPAWASEHQSTLSTGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYA"
     gene            complement(285..>385)
                     /locus_tag="ACHKUC_005410"
     CDS             complement(285..>385)
                     /locus_tag="ACHKUC_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621412.1"
                     /translation="SAWWVNGWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT           99 a          100 c           90 g           96 t
ORIGIN      
        1 tggcataact gaaggacgta atcagcccca gcgtgtccgt aaactcataa cggtatttca
       61 cgttaatccc gttcagatta tcgctgccgg gagcgttcgt acgggcatga agataccctg
      121 tgctcagtgt ggactgatgt tccgacgccc atgcaggcgc accggatacg gacagacaga
      181 tggctgcgga caaaatggct gcacataatt tacgcataat tacctctcgc ttttctgcaa
      241 taaaaaaggc gccatttctg gcgcccgtat tggggttata aaattcagct aatcgtgatg
      301 cctgcagtgg ctttcttcat cacaacaacc agcaaatcgc tgatacttgc tgtgggatac
      361 cagccattta cccaccatgc tgata
//
LOCUS       DAZFRG010000707          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZFRG010000707 DAZFRG010000000
VERSION     DAZFRG010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.710"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          115 a           65 c          118 g           83 t
ORIGIN      
        1 cgtggttatc gggcggcagg ggtaaaaaga ataaaaaaat cccgcagtga tcgcggagct
       61 gcggggacag acaaagatta gagttaagga gttgtttttg ttactcgggc aaaaaacact
      121 aacgcagcga aattatacgc gccacagtca gtttgtgaaa atgtgaagat attcagaatt
      181 tttattcagt catgatacag gcatcctccg gggtgcctgt tgttttttgg gcataaacag
      241 attcagacat cagacaggag agggggagcg agtgggtaaa ggtggcggta aggcgcacac
      301 accgcgcgag gcgaaggata atctcaaatc cacgcagatg atgagtgtga ttgatgcgat
      361 tggtgaggga ccgatagaag g
//
LOCUS       DAZFRG010000708          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZFRG010000708 DAZFRG010000000
VERSION     DAZFRG010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.711"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..288)
                     /locus_tag="ACHKUC_005411"
     CDS             complement(<1..288)
                     /locus_tag="ACHKUC_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621413.1"
                     /translation="MSKYIYILLSFLVLFFIFFYAYISLMSKEHHYTQHELSPFFLYT
                     PESLRNLPNISNVAEYSYYYNVDDMQTRVIVTWRNIDNIFLQKAKLIDFLKR"
BASE COUNT          137 a           56 c           63 g          120 t
ORIGIN      
        1 tcttttcaaa aaatctatta gctttgcttt ctgaagaaaa atgttatcta tatttcgcca
       61 tgtaacaatc actcttgttt gcatatcatc aacattatag taatagctat actcggctac
      121 gtttgatatg tttggcaaat ttcgcaaact ctctggcgta taaaggaaaa atggggaaag
      181 ttcatgctgt gtatagtgat gttcctttga catcaatgag atataagcat agaaaaatat
      241 aaagaataaa actaaaaatg ataataaaat ataaatatat tttgacatct ctactcacga
      301 cctgtgcgga taatgagggt ttaagaaaag aaaaagcggt ctttcaaagg gagattctga
      361 aaactttata gctccc
//
LOCUS       DAZFRG010000709          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZFRG010000709 DAZFRG010000000
VERSION     DAZFRG010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.712"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(174..>375)
                     /locus_tag="ACHKUC_005412"
     CDS             complement(174..>375)
                     /locus_tag="ACHKUC_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621414.1"
                     /translation="KGGMNLNGLPSSTNVIDLRGKDMGTYIDANGTCWAPGTPSIIMY
                     PGGSGPSYSMSSSTSSVNSGTS"
BASE COUNT          109 a           78 c           72 g          116 t
ORIGIN      
        1 ttaataaaat ttttgcttta tactgtagat ttcacgtgtg taagtaatat atgaaaaggc
       61 tctcgttaag atgtctggta caaaatgaca gatcatatgc ctttgttaag agacgacaga
      121 agtagagtct tacaggcaca tcataaatac cctccgacca tgccggaggg tatttatgat
      181 gtgccgctgt ttacgctgga tgtggaacta ctcatactat aagaaggtcc acttcccccc
      241 ggatacatga tgatggatgg agtacccgga gcccagcatg ttccattagc atcaatatat
      301 gttcccatat cttttcctcg tagatctatt acattagtag aactaggtaa tccattaaga
      361 ttcatccctc ctttc
//
LOCUS       DAZFRG010000710          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZFRG010000710 DAZFRG010000000
VERSION     DAZFRG010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.713"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>372
                     /locus_tag="ACHKUC_005413"
     CDS             <1..>372
                     /locus_tag="ACHKUC_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kfiB protein"
                     /protein_id="HFO6621415.1"
                     /translation="IENQEIKKKLPPVLYGAAEQIKQELGYRLGYIIVSYSKSLKGII
                     TMPFALIRECVFEKKRKKSYGVDVPLYLYADADKAERVKKHLSYQLGQAIISSANSIF
                     GFITLPFKLIVVVYKYRRAKI"
BASE COUNT          122 a           55 c           72 g          123 t
ORIGIN      
        1 tatagaaaat caggaaatta aaaagaaact tccacctgtg ctatatggag cagctgagca
       61 gataaaacaa gagttaggtt atcgacttgg ttatattata gtctcgtatt ctaaatccct
      121 caaggggatt attaccatgc catttgcact tatccgtgag tgtgtttttg aaaaaaaacg
      181 taagaagagt tatggcgttg atgtgccact ctatttatat gctgatgctg ataaggctga
      241 aagagttaag aaacatttat cttatcaatt agggcaggct attatctcca gtgctaattc
      301 gatatttgga ttcattaccc ttccatttaa gttaattgtt gttgtttata aatataggag
      361 agctaaaatc aa
//
LOCUS       DAZFRG010000711          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZFRG010000711 DAZFRG010000000
VERSION     DAZFRG010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.714"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..138
                     /locus_tag="ACHKUC_005414"
                     /pseudo
     CDS             1..138
                     /locus_tag="ACHKUC_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154256.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
BASE COUNT          107 a           76 c           83 g          104 t
ORIGIN      
        1 ttaacagcta tccgtatggc gtgttcggtt ttgcgaataa aaaatggtta ctgtagaagc
       61 ggatttccac cacatgtcaa ccatgaatgg tcagtggtgc gagacgactg ggatgatttg
      121 gtcgatgact tcttccggcg aatactgata gaaacattga aatattgtaa gagaaagcgt
      181 tcaaaattct gtggcacgtg aaaaattcta cagcgtcatc accttattca gtcgaccttc
      241 aaaataaacg tttaacagag acaatcacca tttggattgt ccgctgagga ggcgaacgat
      301 ttcctagaca gctttatcat gtcagcccac attgttggcg ctgacaatgg atttgcccct
      361 atatttccag
//
LOCUS       DAZFRG010000712          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZFRG010000712 DAZFRG010000000
VERSION     DAZFRG010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.715"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..234
                     /locus_tag="ACHKUC_005415"
                     /pseudo
     CDS             82..234
                     /locus_tag="ACHKUC_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
BASE COUNT          121 a           69 c           92 g           86 t
ORIGIN      
        1 ccgatataac cttaagactg gagttgcaac tggagctcgt cgaaaatcaa cgcccaaatc
       61 ctcgacaaat ggagaaagag aatgagcaat atcaccagga atagtttagt tgtagcaggc
      121 gttctggctg cgcttatggc agggaatgtt gcgttggcat ctgatgtgcc tactgaagtg
      181 actctaatag aaaaacaaac gctggtacgt aacaacggtg cagaggttca gtcagagcag
      241 gacactgact gtcgtctgag aggaagtgga taaagccgga gcaagagagc caaatcatga
      301 tcgattcgaa tgccaaacta tgatcgattc agatgccatt ttgcaattaa tttaaagttt
      361 tataaaaa
//
LOCUS       DAZFRG010000713          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZFRG010000713 DAZFRG010000000
VERSION     DAZFRG010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.716"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>363
                     /locus_tag="ACHKUC_005416"
     CDS             <1..>363
                     /locus_tag="ACHKUC_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6621416.1"
                     /translation="KYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDE
                     RLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRAT
                     TNSNHNLPVAPNLLNQTFA"
BASE COUNT          110 a           87 c           95 g           71 t
ORIGIN      
        1 aaaatacgcg ttaatcgaac aatggcgaca acaatttccc attgaagcga tgtgtcaggt
       61 atttggtgta tccaggagcg gttattacaa ctgggtacag catgaaccct cagacagaaa
      121 acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca ctcgcgaaac
      181 atatggaacc cggcggctcc agacggagct ggcagagaat ggcatcatcg ttggtcgtga
      241 ccgactggca cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag
      301 agcgactacg aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt
      361 cgc
//
LOCUS       DAZFRG010000714          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZFRG010000714 DAZFRG010000000
VERSION     DAZFRG010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.717"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..350)
                     /gene="tssD"
                     /locus_tag="ACHKUC_005417"
     CDS             complement(<1..350)
                     /gene="tssD"
                     /locus_tag="ACHKUC_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6621417.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHV"
BASE COUNT          107 a           86 c           62 g          108 t
ORIGIN      
        1 tttacatgaa tagcagttaa tgaagcattt cttagctgaa tataataata ccgttcccag
       61 cgcccgcttt tatttatacg atacaaatcg atttcaataa ataaccgctc attctgattg
      121 atggcattag acagtagcgg tgagctttta tcaatgagtt tgcaaaattg tagcccctgt
      181 agattaacgc ctttaccagt actggtaatg gcgttggtaa gtgcaaaggc aaaaatttca
      241 tcttcatggc caacctgcca gcgattacca accgacgcat acgtcccaca gcctgaagaa
      301 atatcgccct gcttctcacc gattattctc aggtaaacaa tatcgcccac tttatttcat
      361 tcc
//
LOCUS       DAZFRG010000715          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZFRG010000715 DAZFRG010000000
VERSION     DAZFRG010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.718"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>362
                     /locus_tag="ACHKUC_005418"
     CDS             <1..>362
                     /locus_tag="ACHKUC_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO6621418.1"
                     /translation="SIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLY
                     LDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYV
                     NLKKWHEANLTPYLLTLL"
BASE COUNT          105 a           70 c           68 g          119 t
ORIGIN      
        1 ctcaattatt gtatatttaa ttgacccaaa atactttgct gatctgccga cgtcacagtt
       61 ttggtcgtac gcgacatact tcagggtatt gtcctttgaa tatcttagtg aaagtatttc
      121 cacactgctg tatctggatg ccgatgtcgt ttgtaaagga agtctgaaac ctctcacaaa
      181 aattatattt aaagatgagt ttgctgcggt tattcctgac aatgatagta ctcaggcggc
      241 atgtgcaaaa cgtcttaaca ttcccgaaat gaatggacgt tatttcaatg caggcgttat
      301 ctatgtcaat cttaaaaaat ggcatgaagc aaatttgaca ccgtatttac tcacgctttt
      361 ac
//
LOCUS       DAZFRG010000716          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZFRG010000716 DAZFRG010000000
VERSION     DAZFRG010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.719"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          101 a           51 c           91 g          119 t
ORIGIN      
        1 ggttctctgg gatgaatcag taaaaaaagt taagtggatt tatgagaaat agcaccattt
       61 aactgaaagc taccataacg tctttttgat cacaggcgtg ataatgcgtc tcatttaaat
      121 ttcgtttttt gggtcatgga aattttccga acatcagtga taatgagggt tttgttgttt
      181 ttgtttctgc aatgtttgag ggctgttcct ggagacgatt cgttgcttgt gagcaagtgg
      241 tggtccgctt tactggacga gttttgcatt tgttgagtgg tgaaatggaa gtaaaaagag
      301 cccctttctg gcaatgactg aaaaagtcag tagcgtgtga cagtaaaaac taacataaag
      361 tg
//
LOCUS       DAZFRG010000717          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZFRG010000717 DAZFRG010000000
VERSION     DAZFRG010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.720"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>357
                     /locus_tag="ACHKUC_005419"
     CDS             34..>357
                     /locus_tag="ACHKUC_005419"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFO6621419.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQ"
BASE COUNT          100 a           82 c           90 g           85 t
ORIGIN      
        1 tagggaaaat cacacctgaa tcaggaatta acaatgaaac ctgtaaaacc acctcgtatt
       61 aatggacgag tgccggtcct gtcggcacag gaagcggtga attatattcc cgacgaagca
      121 acactttgtg tgttaggcgc tggcggcggt attctggaag ccaccacgtt aattactgct
      181 cttgctgata aatataaaca gactcaaaca ccacgtaatt tatcgattat tagtccaaca
      241 gggcttggcg atcgcgccga ccgtggtatt agtccgctgg cgcaagaagg tctggtgaaa
      301 tgggcattat gcggtcactg gggacaatcg ccgcgtattt ctgatctcgc agaacaa
//
LOCUS       DAZFRG010000718          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZFRG010000718 DAZFRG010000000
VERSION     DAZFRG010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.721"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>355
                     /locus_tag="ACHKUC_005420"
     CDS             <1..>355
                     /locus_tag="ACHKUC_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621420.1"
                     /translation="CQHLITNTEFSYLALPENYNRLFLPNSKNKTNNGLKTLNSEAVG
                     RLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVD
                     TYTEINSYLGKLRCQQ"
BASE COUNT          134 a           65 c           67 g           89 t
ORIGIN      
        1 tgccaacatc ttatcactaa caccgagttc agctatttgg cactccctga aaattataac
       61 cgcttgtttt taccaaattc aaaaaacaaa acaaataatg gtttaaaaac gttaaacagc
      121 gaagctgttg gcaggctact tgctgctggt ggcgtataca atgggaacat tgaaggattc
      181 agagatactg cggaaaaact gggtggagat gcaatcaaag gctatgatca aatactaaat
      241 gaaaaaacag cgggcatagc gatagcaaca gcatctattc ttttaacaaa gcgttctaat
      301 gttgatacat atacagaaat aaatagttac ttaggcaaac ttagatgcca acaaa
//
LOCUS       DAZFRG010000719          354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZFRG010000719 DAZFRG010000000
VERSION     DAZFRG010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.722"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..240)
                     /locus_tag="ACHKUC_005421"
     CDS             complement(<1..240)
                     /locus_tag="ACHKUC_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFO6621421.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPCETVNGGTLVNHDNQFVSGTA"
BASE COUNT           86 a           94 c          100 g           74 t
ORIGIN      
        1 agctgttccg gatacaaact ggttgtcatg gtttaccagt gttccgccat tcactgtttc
       61 acacgggtgc acaacgatgt cagcagccag caccgggagt gacgtgaccg cggcaagaga
      121 cagtgccacc gccacaccgc cacgtttacc ccgtgcgcgg gccagttcgg aggcaaccac
      181 gaaagcgccc gtaatgtgat tccataccag cctgtagcag gtattcagat gtcgtttcat
      241 cagcatttcc ttaatcagag tgagggtgaa taaaaaagcc ggtatccaca gatgtggata
      301 ccggcaggta aagggttaca gtgccggagg tccggctgac ggaagtttct gacg
//
LOCUS       DAZFRG010000720          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZFRG010000720 DAZFRG010000000
VERSION     DAZFRG010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.723"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>347
                     /locus_tag="ACHKUC_005422"
     CDS             <1..>347
                     /locus_tag="ACHKUC_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6621422.1"
                     /translation="DKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSIS
                     ASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYY
                     EFLRLGREGYTKV"
BASE COUNT           66 a          102 c           99 g           80 t
ORIGIN      
        1 gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc cgccagcggt
       61 ggcttcctgg caccgttcgt cgccccggat atcgtctggg acttccgcct gccgcgtgtg
      121 aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg cggctgggtt
      181 atctggcgtg atgaagaagc gctgccgcag gaactggtgt tcaacgttga ctacctcggc
      241 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
      301 tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtaca
//
LOCUS       DAZFRG010000721          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZFRG010000721 DAZFRG010000000
VERSION     DAZFRG010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.724"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          103 a           66 c           63 g          105 t
ORIGIN      
        1 agttttgaga catctctatg agtctcagga cagttaaaaa ataaaaatta tttttacccg
       61 gtcactttgt aaaaaagtgg tcgggttttt gatttctgtg tttcgtgcgc ttaatcgagt
      121 ctcacgcttc agtgagcaaa ttgcccctcc ccaacagaca tcacgattca caaggtcaaa
      181 aattctgttc catgaagtac cgtacaaaga taaagagaaa aatgccttat gcgggatgta
      241 gctgtcgcgt ctttatcggg aaactatacg taattgccac tttgtcttcg gtaatcagaa
      301 aaagaagacc tgccatttta ataaatatta tttctct
//
LOCUS       DAZFRG010000722          330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZFRG010000722 DAZFRG010000000
VERSION     DAZFRG010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.725"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>330
                     /locus_tag="ACHKUC_005423"
     CDS             <1..>330
                     /locus_tag="ACHKUC_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6621423.1"
                     /translation="NKGLEGLTWDGNNQNLFAAKEMKDVTITRANMISNDMGIDKIID
                     PVAKSLKDVSGLHYHTPTSSLLILSHESPKIIEVNNEGRITDRLYLESGWSGLTESVP
                     QAEGITL"
BASE COUNT          111 a           51 c           78 g           90 t
ORIGIN      
        1 taataagggg ttggaaggat tgacatggga tggaaataat cagaatctgt ttgcagcaaa
       61 ggagatgaaa gatgtcacaa ttaccagagc taatatgata agtaatgaca tgggaattga
      121 caaaatcatc gatccggtcg ccaagtcttt aaaggatgta tcaggattgc attatcatac
      181 accaacatct tcgcttttaa ttctttctca tgaatcccca aaaattatag aggttaataa
      241 tgagggtaga attacagata gattgtatct ggagtctggt tggtccggtt tgacagaaag
      301 tgtcccgcag gccgaaggga taactctaga
//
LOCUS       DAZFRG010000723          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZFRG010000723 DAZFRG010000000
VERSION     DAZFRG010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.726"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..146)
                     /locus_tag="ACHKUC_005424"
     CDS             complement(<1..146)
                     /locus_tag="ACHKUC_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFO6621424.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFR"
     gene            complement(157..>329)
                     /locus_tag="ACHKUC_005425"
     CDS             complement(157..>329)
                     /locus_tag="ACHKUC_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6621425.1"
                     /translation="YRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNVGN
                     FGGFLSINKLSQ"
BASE COUNT           79 a          104 c           77 g           69 t
ORIGIN      
        1 atacgaaaat acgcctccgg ctctgcggaa atattcacac aagggatata ccgctccccc
       61 tccggcgtgc ttatcacgaa gccgcacgac tccgcaggcg cacaccgccg ggcatgcgcc
      121 agaatcgctg attcagtctg tgtcataaac cgggatttac tgcgaaagtt tattaatgga
      181 aaggaaaccg ccaaaattgc cgacattcct gcgcagttca cacccgcgca tgcacttgct
      241 gcatctgtcc ttacggatat ccgtggtggg tttatcgaac tcatccgcca ctgccccgcc
      301 cgtgtaacca cactcatcag agcggtagg
//
LOCUS       DAZFRG010000724          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZFRG010000724 DAZFRG010000000
VERSION     DAZFRG010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.727"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>320
                     /locus_tag="ACHKUC_005426"
     CDS             <1..>320
                     /locus_tag="ACHKUC_005426"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6621426.1"
                     /translation="IAPYFSACDIFVLSSRWEGFGLVVAEAMSCERIVVGTDSGGVRE
                     VIGDDDFLVPISDSTQLASKIEKLSLSQIRDHIGFRNRERILKNFSIDTIIMQWQELY
                     GTII"
BASE COUNT           88 a           48 c           71 g          113 t
ORIGIN      
        1 atattgctcc ctatttttct gcatgtgata tttttgttct ctcttctcgt tgggaaggat
       61 ttggattagt cgtggcagaa gctatgtcat gtgagcgaat tgttgttggc acggattcag
      121 ggggagtaag agaagttatt ggtgacgatg attttcttgt acccatatct gattcaacac
      181 aacttgcaag caaaattgaa aaattgtctt tgagccagat acgtgatcac attggttttc
      241 ggaatcgtga gcgtatttta aaaaatttct caatagatac tattattatg cagtggcaag
      301 aactctatgg aactataatt
//
LOCUS       DAZFRG010000725          315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZFRG010000725 DAZFRG010000000
VERSION     DAZFRG010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.728"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..251)
                     /locus_tag="ACHKUC_005427"
     CDS             complement(<1..251)
                     /locus_tag="ACHKUC_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFO6621427.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRM"
BASE COUNT           89 a           77 c           71 g           78 t
ORIGIN      
        1 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
       61 ctcactaaat ctgcctgacg aatgaccagc tcagatttat ccaacgccag ctgagcaaaa
      121 gttcggagct gattattcac ttctgatttt gcccaaaaga aggcaatcca tagcgacaga
      181 atgatgggaa agagaactaa aaagataatg cccggaaacg ccagtaattg gtgtcgtgca
      241 cgatgactca ttgctatcaa ttccctgcct gtttataccc ggttattgca aggtgaaacc
      301 ctggctaccg acgtc
//
LOCUS       DAZFRG010000726          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZFRG010000726 DAZFRG010000000
VERSION     DAZFRG010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.729"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>314)
                     /locus_tag="ACHKUC_005428"
     CDS             complement(<1..>314)
                     /locus_tag="ACHKUC_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621428.1"
                     /translation="PELLGGVAWIESGGMPENYKFQIYETKRMIGLLDMPENKTSFGS
                     MGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFQIAATYLRDLVLFDYPSSAT
                     LY"
BASE COUNT           95 a           65 c           56 g           98 t
ORIGIN      
        1 tatacagtgt tgccgaagag ggataatcaa aaagaacaag atctcttaaa taagtagcag
       61 caatttgaaa ggtaaaatca tcctccatta ggcatgtcgc taactctaac tgatttctgg
      121 tagttaattc tgaagggtct agacctagag taatcgctgc ggttcgtatt tgtataccca
      181 ttgagccaaa tgatgtttta ttttcaggca tatctaacaa tccgatcatt ctttttgttt
      241 catatatctg aaacttataa ttttcaggca tacctcctga ctcaatccag gccacgccgc
      301 ccagtaactc tgga
//
LOCUS       DAZFRG010000727          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZFRG010000727 DAZFRG010000000
VERSION     DAZFRG010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.730"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..229
                     /gene="fumD"
                     /locus_tag="ACHKUC_005429"
     CDS             20..229
                     /gene="fumD"
                     /locus_tag="ACHKUC_005429"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO6621429.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          103 a           54 c           82 g           73 t
ORIGIN      
        1 tgataaatag gtataaagaa tgggaaaccg gacaaaagag gatgaactgt accgagaaat
       61 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
      121 tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc agcgcagtga
      181 gctggaaaag caggcgatgg aaacagtcat taacgcactg gtaaaatgac cattcaggat
      241 gaggcggtaa gcttcatcct gatattgtta gttcagttgc ggtccagtta atcattctaa
      301 ataacaactg ct
//
LOCUS       DAZFRG010000728          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZFRG010000728 DAZFRG010000000
VERSION     DAZFRG010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.731"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..218)
                     /locus_tag="ACHKUC_005430"
     rRNA            complement(<1..218)
                     /locus_tag="ACHKUC_005430"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           69 a           85 c           62 g           95 t
ORIGIN      
        1 tttcctcggg gtacttagat gtttcagttc ccccggttcg cctcattaac ctatggattc
       61 agttaatgat agtgtgtcga aacacactgg gtttccccat tcggaaatcg ccggttataa
      121 cggttcatat cacctcaccg acgcttatcg cagattagca cgtccttcat cgcctctgac
      181 tgccagggca tccaccgtgt acgcttagtc gcttaacctc acaacccgaa gatgtttctt
      241 gcgatccatc atcgtgttgc gaaaatttga gagactcacg aacaactctc gttgttcagt
      301 gtttcaattt t
//
LOCUS       DAZFRG010000729          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZFRG010000729 DAZFRG010000000
VERSION     DAZFRG010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.732"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..230)
                     /locus_tag="ACHKUC_005431"
     CDS             complement(<1..230)
                     /locus_tag="ACHKUC_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621430.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVM
                     NIKSILVLVCCATILSLIMIIFIFFITSFFIP"
BASE COUNT          121 a           55 c           40 g           91 t
ORIGIN      
        1 attggtataa aaaaagatgt tataaaaaaa ataaaaatga tcataatcaa tgatagaata
       61 gttgcacaac aaaccaatac caagatgctt ttaatattca tcacatcaaa gaaaatatcc
      121 agtcgatcca gacgactgaa atatccccag agaactatca acgcaatccc tgatataata
      181 cttgatggtg ttaatactga aactattagc gtcttccact tctgattcat tttaacttcc
      241 tggttttgct ataaagagag cataaaaaag actgaataag ttattacact tcattgtttg
      301 actcctc
//
LOCUS       DAZFRG010000730          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZFRG010000730 DAZFRG010000000
VERSION     DAZFRG010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.733"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>306
                     /locus_tag="ACHKUC_005432"
     CDS             <1..>306
                     /locus_tag="ACHKUC_005432"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6621431.1"
                     /translation="NINIEKNKKIKDRSGIEREFDIYWEFEIGGHTYRSVIECKDYSS
                     PVSIEKIDAFIGKTNDIPGLKLIYATRTGYQSGAKIKAEQHNIQLLVIRDQQEQDWVD
                     "
BASE COUNT          107 a           46 c           73 g           80 t
ORIGIN      
        1 aacattaata tcgagaaaaa taagaagata aaagatcgtt cgggaattga acgagaattt
       61 gatatatatt gggagttcga aattggaggt catacctatc gttcggttat cgagtgtaag
      121 gactattcct ccccggtatc gattgagaag atagatgctt tcattggtaa aactaatgat
      181 attccgggac ttaaactaat ctatgcgacg cgaacggggt atcaaagtgg tgcaaagata
      241 aaagcagagc agcataacat tcaactgctt gttatcaggg atcagcaaga acaagactgg
      301 gttgat
//
LOCUS       DAZFRG010000731          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZFRG010000731 DAZFRG010000000
VERSION     DAZFRG010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.734"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..>306
                     /locus_tag="ACHKUC_005433"
     CDS             19..>306
                     /locus_tag="ACHKUC_005433"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO6621432.1"
                     /translation="MSAGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRA
                     RNDQHQFDGYVTYKINDSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQY"
BASE COUNT          101 a           54 c           67 g           84 t
ORIGIN      
        1 gggattcaga cacaatggat gtcagcagga agtcagtata acccttatct gaaaatttcg
       61 tatcaggcaa cgccagatct taatctggcc ttacgctatc gctatgactg gaaagctttc
      121 cgacagacgg atttagatgg caacagggcg cgtaatgatc agcatcagtt tgatggttat
      181 gtgacataca aaattaatga ttcatggttg tttgcatggc agaccacggt atatacgaag
      241 gttaataatt tcaaatatgg gaatcataaa aaaacagcaa ctgaaaatgc atttgttctg
      301 caatat
//
LOCUS       DAZFRG010000732          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZFRG010000732 DAZFRG010000000
VERSION     DAZFRG010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.735"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>303
                     /locus_tag="ACHKUC_005434"
     CDS             <1..>303
                     /locus_tag="ACHKUC_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6621433.1"
                     /translation="YLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEE
                     GARWGWLDDNTILLTSVLALVSFILFAARTLKISNPIMDLHAFKDKNFTLGCFFSFS"
BASE COUNT           73 a           50 c           62 g          118 t
ORIGIN      
        1 atatttagtt ttgagtattc cttttctggt taattttgat aaacctgatc tctctttact
       61 gaaagttgct gattatccga gtattatcct gttggccatg acactgggat gtctggaata
      121 tacccttgaa gaaggagcgc gctggggatg gctggatgat aatactattt tgctgacatc
      181 agttctggca ttggtgagtt ttattttatt tgctgcgcgt acacttaaaa tatccaatcc
      241 gataatggat cttcatgctt ttaaggataa aaatttcaca ctgggatgtt tcttttcttt
      301 ttc
//
LOCUS       DAZFRG010000733          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZFRG010000733 DAZFRG010000000
VERSION     DAZFRG010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.736"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..270
                     /locus_tag="ACHKUC_005435"
     CDS             <1..270
                     /locus_tag="ACHKUC_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO6621434.1"
                     /translation="DRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIARE
                     QSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          106 a           57 c           56 g           83 t
ORIGIN      
        1 gatcgtagtc atcgaaagta tcagcaaacc attaccgata aaacagtatt gattcactat
       61 acagggataa ctaaaccatg gcatagctgg gctggatatc cgtctgcatc atactttaat
      121 atcgcgcgtg aacaatctcc ctggaagaaa tatcctctta aagaggcgcg gactgttgca
      181 gaaatgcaga aacaatataa gcatctgttt gcccatggtg agtatattaa aggtataact
      241 tcattaatta agtacaagct taagaaataa attactctta tgattacaag tatacgctat
      301 cg
//
LOCUS       DAZFRG010000734          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZFRG010000734 DAZFRG010000000
VERSION     DAZFRG010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.737"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>301)
                     /locus_tag="ACHKUC_005436"
     CDS             complement(<1..>301)
                     /locus_tag="ACHKUC_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6621435.1"
                     /translation="SELARRVCKGSTRRGKRLSVLTSLALSALLPTVAGASTVGGNNP
                     YQTYRDFAENKGQFQAGATNIPIFNNKGELVGHLDKAPMVDFSSVNVSSNPGVATL"
BASE COUNT           75 a           68 c           68 g           90 t
ORIGIN      
        1 taatgttgca acgccgggat ttgagcttac attcacactg ctaaaatcaa ccatgggcgc
       61 tttatcaaga tgtcctacta attccccttt attattaaaa ataggaatgt ttgttgcgcc
      121 agcctgaaac tgccctttgt tttctgcaaa gtcgcggtat gtctggtaag gattgttgcc
      181 accaaccgtt gatgcaccag caacggttgg gagtaatgca gatagtgcca gagaggtaag
      241 tactgaaagt ctttttcctc tgcgggtact ccctttacat acccttcggg ctagttcaga
      301 g
//
LOCUS       DAZFRG010000735          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZFRG010000735 DAZFRG010000000
VERSION     DAZFRG010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.738"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>297
                     /locus_tag="ACHKUC_005437"
     CDS             <1..>297
                     /locus_tag="ACHKUC_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621436.1"
                     /translation="NALTEANKDGAINQAEHDDLTGKNQAVTEAKTPAQAAVDALPDG
                     QEKSELQNRLDAVDGITVPAVNDQDGNGKPDDQDAALKQAQDLVAAAEGKQTEA"
BASE COUNT           80 a           77 c          110 g           30 t
ORIGIN      
        1 aaaatgcgct gacggaagcc aacaaagacg gtgcaatcaa ccaggcagag catgacgatc
       61 tgaccggtaa gaaccaggcg gtgacagagg cgaagacccc ggcccaggct gcggtggacg
      121 cgctgccgga tggtcaggag aagagcgagc tgcagaaccg actggatgcg gttgacggca
      181 tcaccgtgcc ggccgtgaat gaccaggacg gcaacggcaa gccggatgac caggatgcgg
      241 cgctgaaaca ggcgcaggat ctggttgcgg cggcggaagg caagcagacg gaagcag
//
LOCUS       DAZFRG010000736          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZFRG010000736 DAZFRG010000000
VERSION     DAZFRG010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.739"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>295
                     /gene="kfiA"
                     /locus_tag="ACHKUC_005438"
     CDS             <1..>295
                     /gene="kfiA"
                     /locus_tag="ACHKUC_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K5 polysaccharide biosynthesis alpha-UDP-GlcNAc
                     glycosyltransferase KfiA"
                     /protein_id="HFO6621437.1"
                     /translation="GLLRPRVVNQLGTGTVFLKADQLPSLKYMDGSQRFVDVRFSRYM
                     LENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKETQAIAGYSKLN"
BASE COUNT          103 a           47 c           61 g           84 t
ORIGIN      
        1 gggctattgc gaccgagagt tgtaaatcaa ttaggtacag ggactgtttt tcttaaggca
       61 gatcaattac catctttaaa atatatggat ggttctcaac gattcgtcga tgttagattt
      121 tctcgctata tgttagagaa tgaaattggt atgatatgtg ttcccagaga aaaaaactgg
      181 ctaagagagg tctcatcagg ttcaatggaa ggactttgga acacatttac aaaaaaatgg
      241 cctttagaca tcataaaaga aacacaagca atcgcaggat attcaaaact taacc
//
LOCUS       DAZFRG010000737          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZFRG010000737 DAZFRG010000000
VERSION     DAZFRG010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.740"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>291
                     /locus_tag="ACHKUC_005439"
     rRNA            <1..>291
                     /locus_tag="ACHKUC_005439"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           73 a           72 c           82 g           64 t
ORIGIN      
        1 gccccggtaa acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg
       61 taagttccga cctgcacgaa tggcgtaatg atggccaggc tgtctccacc cgagactcag
      121 tgaaattgaa ctcgctgtga agatgcagtg tacccgcggc aagacggaaa gaccccgtga
      181 acctttacta tagcttgaca ctgaacattg agccttgatg tgtaggatag gtgggaggct
      241 ttgaagtgtg gacgccagtc tgcatggagc cgaccttgaa ataccaccct t
//
LOCUS       DAZFRG010000738          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZFRG010000738 DAZFRG010000000
VERSION     DAZFRG010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.741"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          109 a           63 c           47 g           70 t
ORIGIN      
        1 gccctgagaa caaaaaacag accgataaat atcaataaga taacagcaaa caaaacatta
       61 acatctgcgc tgtacaaact ataaacccat cgccagagag tctttctctc tgaaaaagcc
      121 gcttatcaca gtgcataaat ttgccgctgc tttaatcagc caatattcac tgtgaggtat
      181 ttgctcaagc cggtaacgac caaacggata tttagtcagg ctctgaaaac agttcataca
      241 aaacagaacg tgactgtgat ctattcagca aaaatttaaa taggattat
//
LOCUS       DAZFRG010000739          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZFRG010000739 DAZFRG010000000
VERSION     DAZFRG010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.742"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..253
                     /gene="yjjZ"
                     /locus_tag="ACHKUC_005440"
     CDS             17..253
                     /gene="yjjZ"
                     /locus_tag="ACHKUC_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO6621438.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT           77 a           46 c           88 g           78 t
ORIGIN      
        1 tgaaagagat gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc
       61 gggattgctg attgcagggc tgatgtatgc ggatttatcg ccggatcaat ggcggattgt
      121 cattctgatg ggattggtat tgacgccggt gatgttgtat cacaaacagt tacggcatta
      181 cgttttgcta ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt
      241 gaatcagggg tgataaaaca gtgaagagac aagaaaagaa gtaaagata
//
LOCUS       DAZFRG010000740          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZFRG010000740 DAZFRG010000000
VERSION     DAZFRG010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.743"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..187
                     /locus_tag="ACHKUC_005441"
     CDS             2..187
                     /locus_tag="ACHKUC_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621439.1"
                     /translation="MSNTLWLTSKKTGFPLPAGNHLPGVFGWSGYSCLVISKIIAINS
                     GKLIVHNLVMNITLIFP"
BASE COUNT           74 a           65 c           58 g           90 t
ORIGIN      
        1 attgtcaaac acgttatggc tgacatcaaa aaaaaccgga tttcctctgc cagcgggtaa
       61 tcaccttccc ggtgttttcg gttggtctgg ttactcctgt ctggttatta gcaagataat
      121 tgctataaac agtggaaaac tcatcgtaca taatctggtg atgaacatta cgcttatttt
      181 cccttgaccg gaagaatcag aggctgtggt ttcagactgt ctgccagtac attcctctct
      241 ccgttaaaaa ccataacggg ttcattatct tcgtctgtca gcagatt
//
LOCUS       DAZFRG010000741          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZFRG010000741 DAZFRG010000000
VERSION     DAZFRG010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.744"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..208
                     /locus_tag="ACHKUC_005442"
     CDS             <1..208
                     /locus_tag="ACHKUC_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFO6621440.1"
                     /translation="EHFSLLIEISPIRSKKTIMALKEYLVDGYSKQESCERNNVSISY
                     FCLCLKKLNYTHSIVSALKYFYRE"
BASE COUNT           95 a           45 c           49 g           94 t
ORIGIN      
        1 agaacacttt tcacttttaa ttgaaatatc accaatcaga agtaaaaaaa ccatcatggc
       61 tctaaaggag tatttggttg atgggtattc aaagcaagaa agttgtgaaa ggaacaatgt
      121 ttctattagt tatttttgcc tgtgcctaaa aaaattaaac tatacacaca gtattgttag
      181 tgcgcttaaa tatttctacc gtgagtgaac tgtaactgta gtcttgattc ttacgcttaa
      241 acactgctaa gccgttatgg cttagctgat aatttacatt gca
//
LOCUS       DAZFRG010000742          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZFRG010000742 DAZFRG010000000
VERSION     DAZFRG010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.745"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..>283
                     /locus_tag="ACHKUC_005443"
     CDS             90..>283
                     /locus_tag="ACHKUC_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFO6621441.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRY"
BASE COUNT           79 a           63 c           60 g           81 t
ORIGIN      
        1 tgattatccg gatgcgacac atcctttcaa catcatgatg cataataaca tcatgaaata
       61 aaagatgttt tcttacggag tgcacatcta tgtctgataa tcgttcccgg catgatcgcc
      121 tggcggttcg cttatcactc attatcagcc gactgatggc cggagaatct ctgtcactaa
      181 aaacactgtc agatgaattt ggcgttacag aacgtacttt acagcgcgat tttcatcagc
      241 gtctggttca cctggattta gagtacagaa atggcaggta cag
//
LOCUS       DAZFRG010000743          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZFRG010000743 DAZFRG010000000
VERSION     DAZFRG010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.746"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>282)
                     /locus_tag="ACHKUC_005444"
     CDS             complement(<1..>282)
                     /locus_tag="ACHKUC_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621442.1"
                     /translation="KCGNFVNSTDNDAYTANVTPDHIIGVSPDKYVDVVTIRIGQRFV
                     CKPMTGKDSKVSSLCHELTHLVRYGPKGMYGGMQSEDMPVDKELQNAKEY"
BASE COUNT           80 a           62 c           47 g           93 t
ORIGIN      
        1 atactctttc gcattctgga gctctttatc aacaggcata tcctctgatt gcataccgcc
       61 atacatacct ttggggccat atctgactaa atgtgttaat tcatggcaaa gtgatgaaac
      121 tttagaatcc tttcctgtca ttggtttaca tacaaaccgc tggcctattc ttattgtcac
      181 tacatctaca tatttgtctg gagaaacacc tataatatga tcaggggtga cgttagcagt
      241 ataagcatca ttgtccgtag aatttacaaa attgccacac tt
//
LOCUS       DAZFRG010000744          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZFRG010000744 DAZFRG010000000
VERSION     DAZFRG010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.747"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>282)
                     /locus_tag="ACHKUC_005445"
     CDS             complement(<1..>282)
                     /locus_tag="ACHKUC_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621443.1"
                     /translation="IFPGSARVNNGITIGLSPDQYKETLRIEILQNFKKKPFSGRESH
                     VSTLCHELSHFCRYFIDGKHCGGMGTDDVPTEEFDPNFRYTGYARDLVKA"
BASE COUNT           92 a           60 c           46 g           84 t
ORIGIN      
        1 cgcctttact aaatctcgag cgtatcctgt atatctgaag tttggatcaa attcttctgt
       61 tggaacatca tcagttccca ttccgccaca atgtttacca tcgataaaat atctacaaaa
      121 atgagataac tcatggcata gagtagacac atgagattca cgaccagaaa aaggcttttt
      181 cttaaaattt tgaagtatct caattcgtag cgtctcttta tattgatcag gagacagccc
      241 tatagttatc ccattattaa cgcgagccga accaggaaaa at
//
LOCUS       DAZFRG010000745          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZFRG010000745 DAZFRG010000000
VERSION     DAZFRG010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.748"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>280
                     /locus_tag="ACHKUC_005446"
     rRNA            <1..>280
                     /locus_tag="ACHKUC_005446"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           62 c           80 g           51 t
ORIGIN      
        1 aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt
       61 agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta
      121 acttcgggag aaggcacgct gatatgtagg tgaagcgact tgctcgtgga gctgaaatca
      181 gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa
      241 agtggacgta tacggtgtga cgcctgcccg gtgccggaag
//
LOCUS       DAZFRG010000746          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZFRG010000746 DAZFRG010000000
VERSION     DAZFRG010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.749"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHKUC_005447"
     tRNA            24..100
                     /locus_tag="ACHKUC_005447"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHKUC_005448"
     tRNA            143..218
                     /locus_tag="ACHKUC_005448"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           63 c           72 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacc gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZFRG010000747          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZFRG010000747 DAZFRG010000000
VERSION     DAZFRG010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.750"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..185
                     /locus_tag="ACHKUC_005449"
     CDS             <1..185
                     /locus_tag="ACHKUC_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HFO6621444.1"
                     /translation="GGKTAEIAEALAVTDYQARYYLLLLEKAGMVQRSPLRRGMATYW
                     LLKGEKQAGQSCSSTT"
BASE COUNT           82 a           58 c           77 g           58 t
ORIGIN      
        1 atggagggaa aacggcagaa attgcggagg cgctggcggt aacggattac caggcccgct
       61 attacctgtt attactggaa aaagcaggta tggttcagcg ctcaccatta agacggggca
      121 tggcaacata ctggcttctg aaaggagaga agcaggcggg gcagagctgt tcttccacaa
      181 cttaataact caatctgtgg gcatgcttca ctgaaacaga taaatgtcag cgccagtgat
      241 ataaggcgta acgactaaca atcttggcgt tgaca
//
LOCUS       DAZFRG010000748          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZFRG010000748 DAZFRG010000000
VERSION     DAZFRG010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.751"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>275
                     /locus_tag="ACHKUC_005450"
     rRNA            <1..>275
                     /locus_tag="ACHKUC_005450"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           69 a           57 c           95 g           54 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaaccaagg ctgaggcgtg atgac
//
LOCUS       DAZFRG010000749          271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZFRG010000749 DAZFRG010000000
VERSION     DAZFRG010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.752"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           89 a           54 c           58 g           70 t
ORIGIN      
        1 tcaaagattg aaaatcaaga gaaattgtta tagcagtgat acgaaacaac aaaacggaat
       61 gattgccagc cgttagaaaa caccggacac tctcgtgtaa aaagctaccc tgtctatgga
      121 tatagcgata tcgactcatc aagcgtggac tgaatgatat tcagctccat atggacggca
      181 acctggagga taaatacgat ttcgccgttg gaatcaaggt tgttctgcaa tatctaccag
      241 actgcttcaa tttatctgtt ttggcagtgc a
//
LOCUS       DAZFRG010000750          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZFRG010000750 DAZFRG010000000
VERSION     DAZFRG010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.753"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           77 a           35 c           66 g           84 t
ORIGIN      
        1 ttggtaacgt tattagttac agtaatattt ttgtattgta tgtttgatgg cgtcaggtat
       61 attttaaaga tttatgaaaa atatgacgat gtaccaacat ctcttacttt agtgggttta
      121 gggatcaaat tgaggcaaaa acagtatgac cactgagggt gaaaagattg acagtatagc
      181 attaccttcg attgcagatc ctaaggctgt tgtgtggcta ggggacaaca cattcggtat
      241 aggtagcgag ggcgatggct ct
//
LOCUS       DAZFRG010000751          255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZFRG010000751 DAZFRG010000000
VERSION     DAZFRG010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.754"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..103
                     /locus_tag="ACHKUC_005451"
     CDS             <1..103
                     /locus_tag="ACHKUC_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFO6621445.1"
                     /translation="KRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT           57 a           76 c           68 g           54 t
ORIGIN      
        1 aaaacgtatc cacacccgtg ccattcacaa catcgacacc gatattaagc tcaaccgcgc
       61 attgtgggta atggcagaaa cgctgctgga gagcctgcgc tgatgcagca tgtccctgaa
      121 agcgtgattc aggcaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc
      181 atttctgatg atgacctgtg cgcctggtgt acccgtctgc tttaccgtcc cggagaaatc
      241 agtctgtgca gactg
//
LOCUS       DAZFRG010000752          254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZFRG010000752 DAZFRG010000000
VERSION     DAZFRG010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.755"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           84 a           59 c           39 g           72 t
ORIGIN      
        1 tcagtaaaaa aaatgcattt cagacataga gccgagcaac agcacgctct cgcgtatatt
       61 cagcaaaaac gcatttcctg accgactgat gacttcatga tgcgccatgt ttcccccttc
      121 tgtcgggccc ctggatatat gcttccggcc cggaaatact cttcacgcaa taagttaaat
      181 ttagtttttt atgttgtaaa tattgattta aacaatcttt taacccacaa aacaaaatta
      241 aaccagaata aaat
//
LOCUS       DAZFRG010000753          252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZFRG010000753 DAZFRG010000000
VERSION     DAZFRG010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.756"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>252
                     /locus_tag="ACHKUC_005452"
     CDS             <1..>252
                     /locus_tag="ACHKUC_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6621446.1"
                     /translation="TTGTQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYN
                     INGQGFTFKGAGIDIGKESIVNWNALYSSDDVLHKIGPGT"
BASE COUNT           90 a           34 c           58 g           70 t
ORIGIN      
        1 accactggta ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga
       61 aatgtcctgt ttaaggataa tttggatttt ggtagcggtg gtattatctt tgacgaaggc
      121 catgaatata acataaacgg tcagggattt acatttaaag gagcaggaat tgatatcgga
      181 aaagaaagca ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata
      241 ggccccggta ct
//
LOCUS       DAZFRG010000754          252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZFRG010000754 DAZFRG010000000
VERSION     DAZFRG010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.757"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>252
                     /locus_tag="ACHKUC_005453"
     CDS             <1..>252
                     /locus_tag="ACHKUC_005453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6621447.1"
                     /translation="GINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESL
                     AEDPETLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT           64 a           72 c           65 g           51 t
ORIGIN      
        1 tggcattaac gccaatctgt tgttcaaatg gcgacaacaa tggcgcgagg gaaagctgct
       61 attaccttct tcagagagcc cccagctact tcctgtgact ctcgatgcag ctgccgaaca
      121 gccagaatcg ctcgcagagg acccggaaac cctcagtatc agctgtgagg taacgttccg
      181 gcacgggacg ctccgcttca atggcaatgt cagcgaaaag ctcctgactc tgctgataca
      241 ggaactgaag cg
//
LOCUS       DAZFRG010000755          252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZFRG010000755 DAZFRG010000000
VERSION     DAZFRG010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.758"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           79 a           41 c           44 g           88 t
ORIGIN      
        1 tttgcacatt atcccgggaa cgttggaggg ctaatttaaa tgaagtgcaa aaataacaac
       61 agaggactaa accgcggctt ttgcaataca attcttacgc ctgtaggatt agtgagaaaa
      121 cttatagtgc tcatttgaaa ctataaatca tcggtatcat ccctgatttt attgttgaca
      181 ttttatttat gccgactatt tatatggtat acttgtcgaa ttatcttaaa ggaagctcaa
      241 attttcttat tt
//
LOCUS       DAZFRG010000756          251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZFRG010000756 DAZFRG010000000
VERSION     DAZFRG010000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.759"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..251)
                     /locus_tag="ACHKUC_005454"
                     /pseudo
     CDS             complement(72..251)
                     /locus_tag="ACHKUC_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           65 a           62 c           50 g           74 t
ORIGIN      
        1 attatataga gtgtgattac aatctccggc aatgacactg ttagtgcaac agtaatcagg
       61 cctccctctt agtattgctt acgctgaaag aataactggc agcctgccac accatgttgt
      121 ctggcaacga ggaagaccgc cattttcggt tcaaggctct gctgaaaaat agcgattttc
      181 ctgtgtggca cgctgtctgc gtttctctgc ttctaagaca ccaattatct attctccaac
      241 tactagtcta a
//
LOCUS       DAZFRG010000757          249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZFRG010000757 DAZFRG010000000
VERSION     DAZFRG010000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.760"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           73 a           36 c           49 g           91 t
ORIGIN      
        1 aattaaaatt tgtttgattg ttaatggttt gtgctggttg atgactatta attccagaag
       61 gcggtagtct gcattaatat tagcattacg gtgaccagct tttattccgg ccccttcttt
      121 catgaaacaa cgtattccga agaataagtt tgagaatggc agacgaactt ttattattct
      181 gctgaataca gaagatatga attgttcttg tttatgtcag atattcataa cacaggtatt
      241 atggttaac
//
LOCUS       DAZFRG010000758          246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZFRG010000758 DAZFRG010000000
VERSION     DAZFRG010000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.761"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>246
                     /locus_tag="ACHKUC_005455"
     CDS             <1..>246
                     /locus_tag="ACHKUC_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305084.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6621448.1"
                     /translation="EDKEIENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGS
                     NYFDTSSVEAVIRDVTEINPNAIMVVKSTVPVGFTKTI"
BASE COUNT           92 a           47 c           38 g           69 t
ORIGIN      
        1 gaggataagg aaattgaaaa ttatctttca actaaaatac ttaattttcg cgcaactact
       61 aacaaatatg aagcctataa aaatgccaat tacgttatta ttgctacacc aacgaattat
      121 gacccaggtt caaattactt tgatacatca agcgttgaag ctgtcattcg tgacgtaacg
      181 gaaatcaacc caaacgcaat tatggtggtt aaatctacgg tcccagtagg tttcacaaaa
      241 acaatt
//
LOCUS       DAZFRG010000759          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZFRG010000759 DAZFRG010000000
VERSION     DAZFRG010000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.762"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..245)
                     /locus_tag="ACHKUC_005456"
     CDS             complement(<1..245)
                     /locus_tag="ACHKUC_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFO6621449.1"
                     /translation="MLPGSMSEMHFFLLIGISSIHSDRVILAMKDYLVGGHSRKEVCE
                     KYQMNNGYFSTTLGRLIRLNALAARLAPYYTDESSAF"
BASE COUNT           75 a           63 c           46 g           61 t
ORIGIN      
        1 tcaaatgccg atgactcatc tgtataataa ggtgcaagcc ttgctgcaag agcattcagc
       61 cgtataagtc tccccagtgt tgtactgaaa tacccattat tcatctggta tttctcgcag
      121 acctccttac gggagtgccc acctaccaga tagtccttca tagccagaat gaccctgtca
      181 ctgtgaatag acgaaatacc tatcagtaaa aaaaaatgca tttcagacat agacccgggc
      241 aacag
//
LOCUS       DAZFRG010000760          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZFRG010000760 DAZFRG010000000
VERSION     DAZFRG010000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.763"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>245
                     /locus_tag="ACHKUC_005457"
     CDS             <1..>245
                     /locus_tag="ACHKUC_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621450.1"
                     /translation="SFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQL
                     HRLSVVQGMLKHIDFSTASPTVRQALQKGTGAGNISA"
BASE COUNT           50 a           75 c           67 g           53 t
ORIGIN      
        1 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
       61 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      121 caacccagca acttcaccgg ctcagtgttg ttcagggtat gctgaagcat attgattttt
      181 cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca
      241 gtgcc
//
LOCUS       DAZFRG010000761          229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZFRG010000761 DAZFRG010000000
VERSION     DAZFRG010000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.764"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           59 a           43 c           69 g           58 t
ORIGIN      
        1 ccattaccgg ttttaaccaa caggattatc ggtgggcatg aaagaaaacc ccggtatctg
       61 ctgataccgg ggtttctctt tagcatggca gaaatgtgtt tcatgctttt cgggcgaagg
      121 atatccgact tctgtacgga atggcaagtg gcggttaatt tattcagggg aaggctgtat
      181 gggaaaaggt ggcggtaagg cacacacgcc tcgtgaggcg aaggataat
//
LOCUS       DAZFRG010000762          229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZFRG010000762 DAZFRG010000000
VERSION     DAZFRG010000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.765"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>229)
                     /locus_tag="ACHKUC_005458"
     CDS             complement(<1..>229)
                     /locus_tag="ACHKUC_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFO6621451.1"
                     /translation="LCGYVLPAIVTLPALGIMGRKLGPELFGVFTLALAVVGYASIFD
                     AGLTRAVIREVAIEKDNEENKLKIISSATVVI"
BASE COUNT           74 a           55 c           35 g           65 t
ORIGIN      
        1 taattacaac tgtcgctgaa gaaataattt tcaacttatt ttcttcatta tctttttcaa
       61 ttgcgacttc tcgtatcact gcgcgagtaa ggcctgcatc aaaaatgctt gcataaccca
      121 caacagctaa tgccaaagtg aatacaccaa ataattctgg gcctaatttt cgccccataa
      181 tacccaaagc tggtagtgtc acaatagctg gaagtacata cccgcaaag
//
LOCUS       DAZFRG010000763          227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZFRG010000763 DAZFRG010000000
VERSION     DAZFRG010000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.766"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           78 a           52 c           35 g           62 t
ORIGIN      
        1 cacttgaaag actcccctgt tgaaaagcat gtagaagacg acaggaataa gaaaaaataa
       61 gccaagtaaa aagggttacg ttcgctccgt gcgaacgtga tcgcattcag cattaacgaa
      121 tctttttcca ttcacctccc ttctattttt tcataaaatt cttttatgaa cgcccctaaa
      181 ttaccataag caaaatcgct atcattgttc acgtcgaaac aacatag
//
LOCUS       DAZFRG010000764          224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZFRG010000764 DAZFRG010000000
VERSION     DAZFRG010000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.767"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>224)
                     /locus_tag="ACHKUC_005459"
     CDS             complement(<1..>224)
                     /locus_tag="ACHKUC_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6621452.1"
                     /translation="VILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPGSDDLNG
                     INVKYRYEFTDTLGLVTSFSYANAKDEQKT"
BASE COUNT           50 a           62 c           55 g           57 t
ORIGIN      
        1 tgcgtttttt gctcatcttt ggcattggca taactgaatg acgtcaccag ccccagcgtg
       61 tccgtaaact cataacggta tttcacgtta atcccgttca gatcatcgct gccaggcata
      121 tcagtatggg tctgaagata cccggcgctt agtgtggact gatgctctgc tgcgctcgct
      181 ggcgtaccag cggcaaccag ccagactact gcggacagaa taac
//
LOCUS       DAZFRG010000765          218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZFRG010000765 DAZFRG010000000
VERSION     DAZFRG010000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.768"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..128)
                     /locus_tag="ACHKUC_005460"
     CDS             complement(<1..128)
                     /locus_tag="ACHKUC_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6621453.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVA"
BASE COUNT           70 a           42 c           46 g           60 t
ORIGIN      
        1 aatgcaacat catcgcgcat ttcgtgcagc ggaaatcgtt ttgactccgc gatagtagaa
       61 atggcctttg cgccaaaacg tgaatcgagt agttctgagc ggaaatccgt taacagcttc
      121 tggtccattt cgaactcctt aaatttattt gaaggcaata aaaaagtagg atttatccgc
      181 aatggaagca aggtattaca ggcttaattt aaataaca
//
LOCUS       DAZFRG010000766          218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZFRG010000766 DAZFRG010000000
VERSION     DAZFRG010000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.denovo.769"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..>218
                     /locus_tag="ACHKUC_005461"
     CDS             36..>218
                     /locus_tag="ACHKUC_005461"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFO6621454.1"
                     /translation="MKVLAEIKDEKGVHIPFGSVVSIDGTEKKSGFIVDDNGVVYLTG
                     MPNKGTLNVQWGSDNSR"
BASE COUNT           77 a           21 c           53 g           67 t
ORIGIN      
        1 cattagttaa aactattttt gaaacgaata taggaatgaa agtattggct gagataaaag
       61 atgaaaaagg agttcatatt ccttttggta gtgttgtttc aatcgatgga acggagaaaa
      121 aatcaggatt tatcgttgac gataatgggg ttgtttacct cactggtatg cctaataaag
      181 ggactttaaa tgtacagtgg ggaagtgata atagtaga
//
LOCUS       DAZFRG010000767         9887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.guided.180, whole genome shotgun sequence.
ACCESSION   DAZFRG010000767 DAZFRG010000000
VERSION     DAZFRG010000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.guided.180"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..490)
                     /locus_tag="ACHKUC_005462"
     CDS             complement(<1..490)
                     /locus_tag="ACHKUC_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6621455.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVG"
     gene            complement(709..1686)
                     /gene="epmA"
                     /locus_tag="ACHKUC_005463"
     CDS             complement(709..1686)
                     /gene="epmA"
                     /locus_tag="ACHKUC_005463"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6621456.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLLEVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2011..3819
                     /gene="frdA"
                     /locus_tag="ACHKUC_005464"
     CDS             2011..3819
                     /gene="frdA"
                     /locus_tag="ACHKUC_005464"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6621457.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3812..4546
                     /gene="frdB"
                     /locus_tag="ACHKUC_005465"
     CDS             3812..4546
                     /gene="frdB"
                     /locus_tag="ACHKUC_005465"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6621458.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4557..4952
                     /gene="frdC"
                     /locus_tag="ACHKUC_005466"
     CDS             4557..4952
                     /gene="frdC"
                     /locus_tag="ACHKUC_005466"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6621459.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4963..5322
                     /gene="frdD"
                     /locus_tag="ACHKUC_005467"
     CDS             4963..5322
                     /gene="frdD"
                     /locus_tag="ACHKUC_005467"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6621460.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5385..6518
                     /locus_tag="ACHKUC_005468"
     CDS             5385..6518
                     /locus_tag="ACHKUC_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin-hydrolyzing class C beta-lactamase
                     EC-5"
                     /protein_id="HFO6621461.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6607..7140
                     /gene="blc"
                     /locus_tag="ACHKUC_005469"
     CDS             6607..7140
                     /gene="blc"
                     /locus_tag="ACHKUC_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6621462.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7137..7454)
                     /gene="sugE"
                     /locus_tag="ACHKUC_005470"
     CDS             complement(7137..7454)
                     /gene="sugE"
                     /locus_tag="ACHKUC_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6621463.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7630..7776)
                     /gene="ecnB"
                     /locus_tag="ACHKUC_005471"
     CDS             complement(7630..7776)
                     /gene="ecnB"
                     /locus_tag="ACHKUC_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6621464.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGIGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7887..8012)
                     /gene="ecnA"
                     /locus_tag="ACHKUC_005472"
     CDS             complement(7887..8012)
                     /gene="ecnA"
                     /locus_tag="ACHKUC_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6621465.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8064..8630)
                     /gene="efp"
                     /locus_tag="ACHKUC_005473"
     CDS             complement(8064..8630)
                     /gene="efp"
                     /locus_tag="ACHKUC_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6621466.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8672..9700
                     /gene="epmB"
                     /locus_tag="ACHKUC_005474"
     CDS             8672..9700
                     /gene="epmB"
                     /locus_tag="ACHKUC_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6621467.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2527 a         2550 c         2500 g         2310 t
ORIGIN      
        1 cgcccaccgc agtaatgcgg gcaattttat taatcccttt tgaagcaacg acggtgacca
       61 gaatcatcca tgccactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatat
      121 gccagtgctg ggtcatgtcg ctaccgtaga ggaatgttga gaacggtacc caaactttcg
      181 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
      241 caaaacgtgg tccgacacta ttattcatcc aggaatagat accgccttct tctttgcgat
      301 aagcagctcc catttcagcc atcattaagg cgaatggaat aaagaataac aatgcagaaa
      361 atatataaaa gggaatcgca ctataaccca ttaagtaata agccgatggg ctattggcaa
      421 atccaaatac agaagtaaag atcatcaata tgagtcctat cagactcatc ttttttatcg
      481 agtgaggcat gaaaccatcc ttcatttcag cgcgtgtata aaacgcgccc actgaactgc
      541 actgcccaac attgagcggg cagcgtaaaa accaatacag aattgatacc tggatgatat
      601 caaatatcca gtcttaaatt gtggccttta gcggaaaaat gaaaacatat gctacagaat
      661 ggcgcttatc acgccattct tcgctgttaa ttcagtaatt tttcagaatt atgcccggtc
      721 aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca tcaccagacg
      781 atcaacacct aatgccacgc cggaacagtc aggcataccg actttcaagg cttcaatcag
      841 attctggtca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt tatcttgttc
      901 aaagcgttgt tgctgctcac gggcatccgt caattcatgg aaaccattcg ccagctcaat
      961 acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac tgatttgcgc
     1021 cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt tgccaatatt
     1081 tggctctacg ccaaaggtaa acagcaattg tagaagcgtg tcgcggtctt cttcagtatc
     1141 ggcaacgttg ctcaaatcca gtttcgctgc cacttccagc agttgcgttt tgtcggcaga
     1201 tagtgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc tttctgctgc
     1261 cggacagtcc agcacctgtt gcaagagatc gtccacctcg ttcatcaacc ggtacatatc
     1321 atagtgcggt cgataccact caagcatagt gaactcaggg ttgtgataac gccccatctc
     1381 ttcattacgg aagctgcggc acagctggaa taccggccca caaccggcaa ccagcaggcg
     1441 cttcatatgg tattccgggc tggtcattaa ccagagattc atcccctgcg aatgcccggg
     1501 gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg cctggctcat
     1561 acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga tctccgccat
     1621 aatcgccgcg cgttttaata agttaggaat ggatgcgctc ggctgccagg atgccgtttc
     1681 gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca acgcgacggc
     1741 gagacaaatt ttacgcagga ataaaacaac ggtgggcagt gactaaaaaa agcacgatct
     1801 gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt gcgcactaaa
     1861 aaaatcgatc tcgtcaaatt tcagacctat ccatcagact atactgttgt acctataaag
     1921 gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga agtacggggc
     1981 tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga tcttgccatt
     2041 gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc aaatccaaat
     2101 gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt tgctgcagaa
     2161 gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt tcacgataca
     2221 gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt ccaccactgc
     2281 ccaaccgaaa tgacccaact ggaactgtgg gggtgcccat ggagccgtcg tccggatggt
     2341 agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gtacctggtt cgccgccgat
     2401 aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt cccgcagatc
     2461 cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca tgttcgcggc
     2521 ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga tccgtgctaa cgcggtcgtt
     2581 atggctaccg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg catcgttacc
     2641 ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat ggaattcgtt
     2701 cagtatcacc caaccggtct accaggttcc ggtatcctga tgaccgaagg ctgccgcggt
     2761 gaaggtggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta cggcatgggc
     2821 ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc acgcgacaaa
     2881 gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatctccac gccacgtggc
     2941 gatgtggttt acctcgacct gcgtcacctc ggcgagaaaa aactgcatga acgtctgccg
     3001 ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga accgattccg
     3061 gtacgtccga ccgcacacta caccatgggc ggtatcgaaa ccgatcagaa ctgtgaaacc
     3121 cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca cggtgcgaac
     3181 cgtctgggct ccaactcgct ggcggaactg gtggtcttcg gtcgtctggc cggtgaacaa
     3241 gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag cggcaattga agcgcaggca
     3301 gctggcgttg aacaacgtct gaaagatctg gttaaccagg atggcggcga aaactgggct
     3361 aagatccgcg acgaaatggg catggccatg gaagaaggct gcggtatcta ccgtacgccg
     3421 gaactgatgc agaaaaccat cgacaagctg gcagagctgc aggaacgctt caagcgcgtg
     3481 cgcatcaccg acacttccag cgtgttcaac accgacctgc tctacaccat tgagctgggc
     3541 cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa agagtcccgc
     3601 ggcgcgcacc agcgtctgga cgaaggttgc accgagcgtg acgacgtcaa cttcctcaaa
     3661 cacaccctcg ccttccgcga tgctgatggc acgactcgcc tggagtacag cgacgtgaag
     3721 attactacgc tgccgccagc taaacgtgtt tacggtggcg aagcggatgc agccgataag
     3781 gcggaagcag ccaataagaa ggagaaggcg aatggctgag atgaaaaacc tgaaaattga
     3841 ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct atgaagtgcc
     3901 ttatgacgca actacctcat tactggatgc gctgggctac atcaaagaca acctggcacc
     3961 ggacctgagc taccgctggt cctgccgtat ggcgatttgt ggctcctgcg gcatgatggt
     4021 taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg acggtatgaa
     4081 ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata tgacccactt
     4141 catcgaaagt ctggaagcga tcaaaccgta catcatcggc aacgcccgca ccgcggatca
     4201 gggtactaac atccagaccc cggcgcagat ggcgaagtat caccagttct ccggttgcat
     4261 caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag agttcatcgg
     4321 tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc acggtaagaa
     4381 ggagcgtatg gcgcagttga acagccagaa cggcgtatgg agctgtactt tcgtgggcta
     4441 ctgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc agggcaaagt
     4501 agaaagttcg aaagactttc ttatcgcgac tctgaaacca cgctaaggag tgcaacatga
     4561 cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa aaattgccgt
     4621 tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg ttcagcattg
     4681 aactgatttt cggcctgttt gccctgaaaa atggcccgga agcctgggcg ggattcgtcg
     4741 actttttaca aaacccggtt atcgtgatca ttaacctgat cactctggcg gcagccctgc
     4801 tgcacaccaa aacctggttt gagctggcac caaaagcagc caatatcatt gtaaaagacg
     4861 aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg gttgccacca
     4921 tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa tccaaatcca
     4981 aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat gtggagcgcc
     5041 atcattgcgc cggtgatgat cctgctggtt ggtattctgc tgccgctagg gctgtttccg
     5101 ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc agagcttcat tggtcgcgta
     5161 ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat gcaccacgcg
     5221 atgcacgatc tgaaaatcca cgtacctgcg ggtaaatggg ttttctacgg tctggctgct
     5281 atcctgacag ttgtcacact gattggtgtc gttacaatct aacgcatcgc caatgtaaat
     5341 ctggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccctatgttc aaaacgacgc
     5401 tctgcacctt attaattacc gcctcttgct ccacatttgc cgcacctcaa caaatcaacg
     5461 atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc ggtatggcgg
     5521 tagcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg gacatcgcca
     5581 aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc aaaacattta
     5641 cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc gatcccacaa
     5701 caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta ttacatctcg
     5761 caacctacac cgctggcggc ctgccattgc aggtgccgga tgaggtgaaa tcctcaagcg
     5821 acttgctgcg cttctatcaa aactggcagc ctgcatgggc tcctggaaca caacgtctgt
     5881 atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct ggtttgagtt
     5941 ttgagcaggc gatgaaaact cgtgtcttcc agccactcaa actcaatcat acgtggatta
     6001 atgtaccgtc cgcagaagaa aagaactacg cctggggata tcgcgaaggt aaggcagtac
     6061 atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc attgaagata
     6121 tggcccgctg ggtgcaaagc aatttaaaac cccttgatat caatgagaaa acacttcaac
     6181 aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat cagggtctgg
     6241 gctgggaaat gctggactgg ccggtaaatc ctgacatcat cattaacggc agtgacaata
     6301 aaattgcact ggcagcacgc cccgtaaaac cgattacgcc cccaactcct gcagtacgcg
     6361 catcatgggt acataaaacg ggggcgaccg gcggatttgg tagctatgtc gcgtttattc
     6421 cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctaccccaat ccagcgagag
     6481 tcgccgccgc ctggcagatt ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt
     6541 cggacctttt ctccgctttt ccttgctgtc atctacactt agaaaaaaac cagtaaggaa
     6601 acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc
     6661 tgcagttccc ctacgccgcc gcgtggcgtg accgtagtga ataatttcga cgctaaacgt
     6721 tatctcggta cctggtatga gattgcccgt tttgatcacc gctttgaacg tggactggaa
     6781 aaagtcaccg caacatacag cctgcgtgat gacggcggcc tgaatgtcat taataaaggc
     6841 tataaccctg acagagaaat gtggcagcag agtgaaggga aagcgtactt taccggcgca
     6901 ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg ttataacgtt
     6961 attgcactcg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg cgactacctg
     7021 tggatactct cccgcacgcc aaccatttct gacgaagtga aacaggagat gctggcagtc
     7081 gcgacccggg aagggtttga tgtcagtaag tttatttggg tacagcagcc cggtagttaa
     7141 tgagtgctga gtttcagacc aataatcccc aatacgatta acgccagact cgccaggcgc
     7201 atcgggttag cggactcacc gagcagcaca atgccggtga tggccgcgcc gactgcgcca
     7261 atacccgtcc acacggcata agccgtccct actggtaacg atttcatcgc ccaggcaagt
     7321 agcgccagac tgacaatcat cgccgtcacg gtaataacac ttggcgtcaa acgactaaag
     7381 ccgtgagtat atttcaggcc aacggcccat accacttcca gcagaccagc aataactaag
     7441 ataatccagg acatatcagg ctccggaaaa ttggggtcgt ccccggtgaa aagacgcgtt
     7501 tgcaggtcgt cctgcaaagc tgatggttga acggatattt tgccaggtgg caggggaatt
     7561 tcaactatta cctatgtaac tatcgcagaa aaaacaaacg gcacgacaca gctgtcgtac
     7621 cgttattgct tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac cgccatcaga
     7681 aatgtcttca ccaatgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag
     7741 aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt
     7801 atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg atggaaatct
     7861 gatgattttt cggaggagaa gaaaaattag ctggcagcgc gggagatgga gttgccgaga
     7921 tgtttgatat cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgcgctggca
     7981 agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaaaaagtg atggtgcagc
     8041 ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta
     8101 tccactttga tgacttcgcc gatctgtacg aacagcggaa ctttaaccac agcgccagta
     8161 gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta
     8221 tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac
     8281 agagttacga tacactctgc ctgatccagc aaccatttag cgttgtcacc aattgctttt
     8341 gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg
     8401 ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat
     8461 ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat
     8521 gcctggcctt tacccggttt tacgaattca ctcgcttcaa ctgcgtaagg ttcgccgtct
     8581 aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct
     8641 gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc
     8701 atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct
     8761 gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct
     8821 gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga
     8881 tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgc ccggattctc
     8941 caccgacccg ctggaagaac agcatagcgt agtgcctggt ttgttgcata aataccacaa
     9001 ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg
     9061 ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg cacttgagta
     9121 tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc
     9181 gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg
     9241 tctgcggatt cacagccgtc tgccgattgt gatcccggca cgcatcacag atgcgctggt
     9301 tgaacgcttt tcacattcta cgctgcaaat cttgctggtg aatcacatca accatgccaa
     9361 tgaggtagat gaaacattcc gtcaggcgat ggctaagttg cgccgggtgg gcgttacttt
     9421 gctgaaccag agcgttctgt tacgtggtgt gaacgataac gcgcaaacgc tggcaaacct
     9481 gagtaatgcg ttgttcgatg cgggcgtgat gccgtattac ctgcatgtgc tcgataaagt
     9541 acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt
     9601 gctgacattg gtgtcgggat atctggtgcc taaactggcg cgagaaattg gcggcgaacc
     9661 cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgattaaac agggaagttt
     9721 aatctttgcc cgttgcataa cctttaatta ttcatataca ttgctgttgt tactctttat
     9781 ataaccaaca ccaataatta acctttcttt tctgtttata tagagtgagc acggctattt
     9841 atgttaattt aacaactatt actaccaagc ccatttatct ggcttat
//
LOCUS       DAZFRG010000768         6490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.guided.250, whole genome shotgun sequence.
ACCESSION   DAZFRG010000768 DAZFRG010000000
VERSION     DAZFRG010000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.guided.250"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..441
                     /locus_tag="ACHKUC_005475"
     CDS             <1..441
                     /locus_tag="ACHKUC_005475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6621468.1"
                     /translation="QIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQIDESR
                     WKEKLAKSQVRVAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIKSESE
                     LTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            631..1770
                     /locus_tag="ACHKUC_005476"
     CDS             631..1770
                     /locus_tag="ACHKUC_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6621469.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1767..2381
                     /gene="prfH"
                     /locus_tag="ACHKUC_005477"
     CDS             1767..2381
                     /gene="prfH"
                     /locus_tag="ACHKUC_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6621470.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLSSGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(2438..3895)
                     /gene="pepD"
                     /locus_tag="ACHKUC_005478"
     CDS             complement(2438..3895)
                     /gene="pepD"
                     /locus_tag="ACHKUC_005478"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6621471.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4156..4614
                     /gene="gpt"
                     /locus_tag="ACHKUC_005479"
     CDS             4156..4614
                     /gene="gpt"
                     /locus_tag="ACHKUC_005479"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6621472.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            4706..5950
                     /gene="frsA"
                     /locus_tag="ACHKUC_005480"
     CDS             4706..5950
                     /gene="frsA"
                     /locus_tag="ACHKUC_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6621473.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            6008..6409
                     /gene="crl"
                     /locus_tag="ACHKUC_005481"
     CDS             6008..6409
                     /gene="crl"
                     /locus_tag="ACHKUC_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6621474.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTV"
BASE COUNT         1584 a         1583 c         1703 g         1620 t
ORIGIN      
        1 caaataagaa actatcagcc tggtgatttt cagcaactat gcgctatttt ccttcgagct
       61 gttacgatga cggcgagtca gcactattct ccacagcaaa ttgccgcctg ggcgcagatt
      121 gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc agttattaat
      181 gcacaaccgg ttggttttat tacctgcatt ggacattata tcgatatgtt atttgttgaa
      241 ccggaataca cccgacgcgg ggttgccagc gctttgttaa aaccattgat taagtctgaa
      301 tccgaactta cggtggacgc cagcataacc gcaaaaccct tttttgaacg ttatggtttt
      361 cagacagtta agcagcagcg cgttgaatgc cggggaacgt ggtttactaa tttttatatg
      421 cgatataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgt ctagtatgtg
      481 cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct ttatcaatga
      541 ctctttgcag acctttccag gattaattct tttttcttgc cctggattcg tctgccattt
      601 cctgattttt attcataaat ggaatggtac atgggcaaat atattcgtcc cttatccgat
      661 gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat ccaacaatta
      721 cacaccacgg caaatttacc caacatgcag cgcgttgtgg ggatgccaga tttacatccc
      781 ggacgcggct acccgattgg cgcagcgttc ttctctgttg gtcgttttta cccggcactg
      841 gtcggcaatg atatcggctg cggcatggcg ctatggcaaa cagatattct cgctcgcaaa
      901 tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc tgaagaaagc
      961 tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcgcaa ctcgcttggc
     1021 tccatcggtg gcggtaacca cttcgcagaa ctgcaacaga ttgatcaaat tatcgacgcg
     1081 gaactgtttg cactggctgg tctggatgcg cagcatctgc aactgctggt tcatagcggc
     1141 tcgaggggat tggggcagtc tattttacag cggcatattg cctcgttttc gcatcatggt
     1201 ttgcctgaag gcagtgacga cgcgctacgg tatattgcgg aacatgacga tgcgctggcg
     1261 tttgcgcgta ttaatcgcca gctgatcgct ttgcgcataa tgcaacaagt taaggccacc
     1321 ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgccaaat cggcgatcaa
     1381 cagggctggt tgcatcgtaa aggcgccacg ccggatgaca acggtctggt gattattccc
     1441 ggttcacgcg gcgattactc ctggctggtt aaacctgttg cgaacgaaaa aacgctccac
     1501 tcgctggcgc atggggcggg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca
     1561 gcgaaataca cggcgacgca actctcgcgg actgaacttg gcagtcgggt aatttgtcgc
     1621 gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga aagcgtggtg
     1681 caatgtctgg tgctggctgg gttaattatt cctgtcgcgc gactgcgccc ggtgctgacg
     1741 ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctct gctcaggggc
     1801 cggaagaatg ttgtctcgca gtgaaaaaag cactggacag gctgattaaa gaagctaccc
     1861 gacaggacgt cgcggtaacg gtgctggaaa cagaaacggg tcgctactct gacacactgc
     1921 gttcggcgct gatttctctg gatggcgata acgcatgggc tttaagcgaa agctggtgcg
     1981 gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc aaaaactggt
     2041 ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg
     2101 agacgctgcg ttcgtcaggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
     2161 gcgccacgca tctgtcatcc ggtattagcg tgaaggttca gtcagagcgc agtcagcatg
     2221 ctaacaaacg gctggcgcga ttactgattg cctggaagct ggaacaacag caacaggaaa
     2281 atagcgcggc gctgaaatcg cagcggcgaa tgttccatca ccagattgaa cgtggcaacc
     2341 cgcgacgaac gttcacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
     2401 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
     2461 gcagttcagt cagcagtgtc cagtaatggc caacgctttc gatatgaact tgctcatccg
     2521 gagagtgtgg accggtgatg gttggcccga tagacaccat gtccatttcc ggatacggtt
     2581 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggc gttttgttga
     2641 acagacgctg ataggtttca cgtaccagat gcattaccgg agagttagcg tccggctgcc
     2701 agccaggata tgcgcctttt gcttctgttt tcgcacctgc cagtttaccc agtgaatcca
     2761 gcatgctcac tacgtagtct ttaccgctgt cgatcagaga acggatcagg cagtgaattt
     2821 ctacgttatt gtcggtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
     2881 ttgccacgtc ggagttgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
     2941 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc aacagagtcc agcagcaatg
     3001 ccagattttt ctctttctct gccagttcgt ttttcaggat ctcctgatag gtatccacca
     3061 gagatttcag ggcgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
     3121 ggatggcgtt acgcagtgtg ccgccgttga aatcaataag acgcaggtcc agttcttctg
     3181 catgacccgc caggaagcgc accaacagtt tgttggcatt acccaggcca acgtggattt
     3241 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
     3301 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgatacct cccgcacaac
     3361 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
     3421 agttgctctg taagccaaaa gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
     3481 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagtgca gaggccatac
     3541 caatgccgtt atccgcaccc agcgtggtgc cgcgtgcttt aacccattcg ccatcaatat
     3601 aaggctggat aggatcttta gtgaagtcat gcacggtgtc gttgtttttt tgcggcacca
     3661 tatcgaggtg ggcctgcaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
     3721 tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
     3781 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
     3841 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
     3901 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
     3961 gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     4021 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     4081 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     4141 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     4201 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     4261 agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     4321 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     4381 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtaga taccggtggt
     4441 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     4501 ccggccggtc gtccgctggt tgatgattat gttgttgata tcccgcaaaa tacctggatc
     4561 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     4621 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
     4681 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     4741 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttagtc acggcgcaca
     4801 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     4861 ccgtctgatg tggatctggc gcggcattga cccccgtgaa atcctcgatg tccaggcgcg
     4921 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
     4981 ccgcggcggc aactggattt atgagtgggc tacccaggcg atggtgtggc aacaaaaagc
     5041 ctgtgcggaa gaagatccgc aactcagcgg tcgacactgg ctgcatgcgg ctacgttgta
     5101 caacattgct gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
     5161 gtcaaaccgc gcctatgaag aggccgctca gcgtctgccg ggcacgatgc gacagatgga
     5221 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
     5281 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
     5341 cctgtatgaa cgttattttg cgccgcgcgg cattgctatg ctgactattg atatgccgtc
     5401 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
     5461 tttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
     5521 ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagt
     5581 ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca
     5641 ggtgccggaa atgtatcttg acgttctggc cagtcgttta gggatgcatg atgcttccga
     5701 tgaagcgttg cgagtggagt tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg
     5761 tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga
     5821 ggactcacgc ttaatcacgt cgtcatctgc tgacggtaaa ttattagaga tcccatttaa
     5881 cccggtgtat cggaattttg acaaaggcct tcaggaaatc acagactgga tcgaaaaacg
     5941 tttgtgttaa aaatttgcta aattttacca atttggtaaa acagttgcat cacaacagga
     6001 gattgcaatg acgttaccga gtggacaccc gaagagcaga ttagtcaaaa aatttaccgc
     6061 actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct
     6121 ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gagttctggg gctggtggat
     6181 ggaactggaa gcgcaggaat cccgttttac ctacagttac cagtttggtc tgttcgataa
     6241 agcaggcgac tggacgagtg ttcagataaa agatgccgaa gtggttgaac gactggagca
     6301 caccctgcgt gaatttcacg agaagctgcg tgagctgctg gcgacgctga atctgaagct
     6361 ggaaccggca gatgattttc gtgatgaacc ggtgaagtta acggtgtgaa tgaaatgtgc
     6421 cggatgcatc acatccggca atattcatta aaactgatac gtcatcccaa ccgcgacaat
     6481 atcatcatta
//
LOCUS       DAZFRG010000769         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303W23568
            SAMN43283687-rid23067033.guided.473, whole genome shotgun sequence.
ACCESSION   DAZFRG010000769 DAZFRG010000000
VERSION     DAZFRG010000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283687
            Sequence Read Archive: SRR30325476
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351738.1
            Genome Coverage       :: 114x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:52:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,483
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,142
            CDSs (with protein)               :: 5,142
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 3, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 111 of 253
            Pseudo Genes (incomplete)         :: 158 of 253
            Pseudo Genes (internal stop)      :: 61 of 253
            Pseudo Genes (multiple problems)  :: 63 of 253
            Pseudo Genes (short protein)      :: 1 of 253
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283687-rid23067033.guided.473"
                     /serotype="O 18:H 5"
                     /isolate="2303W23568"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKUC_005482"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKUC_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO6621475.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKUC_005483"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKUC_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO6621476.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          427 a          323 c          382 g          453 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgagaatttg ttaaaaaaat cctgcatggt gaatccccct
     1501 gagcggaggg gcatatcagc gtcacaggtg tttctgtttt acctctatcc tttctgtgcg
     1561 ggttcaggtg ctgatactga actca
//
